LOCUS       DASSBW010000001         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_282965, whole genome shotgun sequence.
ACCESSION   DASSBW010000001 DASSBW010000000
VERSION     DASSBW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282965"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFG56_00005"
     CDS             complement(<1..531)
                     /locus_tag="VFG56_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622302.1"
                     /translation="MATKVFVGMSGGVDSSLSAALLKEQGYDVTGIYMKNWTQDLPGM
                     VCPWADDLADAKRVAVQLGIPFKVYDFEEDYKQKVVDYMVEEYQAGRTPNPDIMCNQE
                     VKFKLFLDAALEDGAEMIATGHYARVDDGGWTIDDRQVPEEKNRAVVQHPTSNVQRLL
                     RPKDEVKDQTYFLYRVT"
     gene            complement(521..1135)
                     /locus_tag="VFG56_00010"
     CDS             complement(521..1135)
                     /locus_tag="VFG56_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET6622303.1"
                     /translation="MRLLTMLLIVVVLFVVIVVGLPRYLGPDDLSDCPTTPNQQPVCQ
                     SADAIIAISGGDTAARADEAIELYKNGWAPLVIFSGAAKDKSGPSNAEVMRARAVEQG
                     VPAADILVENLAKNTTENAKNTVEISRNYSLERIILVTSSYHQRRASLEFQAFFGPSV
                     EVVNHPVAHDKQWGRLWWLTPLGWWLALSELAKIIVLSFISYGH"
     gene            complement(1132..2286)
                     /locus_tag="VFG56_00015"
     CDS             complement(1132..2286)
                     /locus_tag="VFG56_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET6622304.1"
                     /translation="MVNQTYLDHAAATPLDERVFQVMQTYLTEQFYNPSAAYSPARQV
                     RSALNDARHRLAMVIGAKETEAILTAGATESINLAIHGVMMGQGGNLIVGATEHASVR
                     AAAEQFDCRVAEADKFGLVSAASIERQVDDHTRLVSLSMADNELGTVQPIKEVAELVR
                     RVKADRLQRGIKTPLYLHSDGSQAAGQLDLNVARMGVDLLTLNAAKCYGPKQTGLLFV
                     KTGLTLEPLIRGGGQERNLRSGTENVANAIGFAESLELAEKRRHQTVRQLADLRHMLA
                     DNLTTAVDGLEIDGHPKRHLPGLLHISLDGLDAERVVFGLDEHGIMVATGAACAANKG
                     TRSVVLDAIGMPPEQADGSLRISLGRSNTNKQLERVAPLIAEVINRERQL"
BASE COUNT          588 a          653 c          578 g          633 t
ORIGIN      
        1 ggttacacgg tatagaaagt aagtctgatc cttaacttcg tcctttggac gcagtagacg
       61 ttggacgttg gatgttggat gttggacgac ggcccggttt ttctcttcag ggacttgtct
      121 atcgtctatc gtccatcctc catcgtctac tctagcgtaa tggccagtgg cgatcatctc
      181 ggccccatct tccagtgcgg catctaagaa tagcttgaat ttgacctctt gattgcacat
      241 gatgtctgga ttaggagtcc gtccggcttg gtattcttcg accatataat caaccacttt
      301 ttgcttgtag tcttcttcaa aatcataaac cttgaatggt atacccagct gaaccgctac
      361 tcgtttagca tcggccaaat catccgccca tgggcaaacc atgccgggta gatcctgagt
      421 ccagttcttc atataaatac cggtaacatc gtaaccctgt tcttttagta gtgctgccga
      481 caatgagcta tcaacgccac ccgacatacc gacgaagact ttagtggcca taagaaataa
      541 acgataagac tattattttt gcgagttccg acagagctaa ccaccaacct agtggtgtca
      601 gccaccacaa tcgaccccat tgcttatcgt gggcaaccgg atgattaaca acctccaccg
      661 acggtccaaa gaaggcttgg aactctaagc tggcccggcg ctgatgataa gatgaggtga
      721 ctaaaattat ccgttccaac gaataattcc ggctgatctc cacggtattt ttagcattct
      781 cagtggtatt ctttgccaga ttttccacca atatatccgc agccggcaca ccttgttcga
      841 cagccctggc ccgcataact tcggcattgg atgggccgga tttatctttg gcggcacctg
      901 aaaagattac cagaggcgcc cagccattct tatacagctc gatagcttcg tcagcacgag
      961 cggctgtgtc gccgccgcta attgcaatga ttgcatcagc cgattggcag accggctgct
     1021 gattgggtgt agtcggacag tcgcttagat catcgggtcc taaataacgc ggtaagccca
     1081 ctacgatgac cacgaacagg actacgacta ttagcaacat tgttaacaac ctcataactg
     1141 tcgctcccgg ttaatcactt cggcgatcag cggggcgacc ctttcgagct gcttgttagt
     1201 attggatcga cccaggctga ttcgcagact accatctgcc tgttcgggtg gcatccctat
     1261 ggcatccaaa actacggagc gcgtcccctt attggcggcg caagctgcgc ctgttgccac
     1321 catgatgccg tgttcatcaa gtccaaacac caccctttca gcgtcaagac cgtctaaact
     1381 aatgtgtagt agacctggta ggtgcctctt aggatgacca tcgatttcta gaccatctac
     1441 cgcggtagtt aaattatcag ctagcatatg gcgtaggtct gctaattgcc taaccgtctg
     1501 atgacgcctt ttttcggcta gctccaggct ttcggcgaat cctatggcgt ttgccacatt
     1561 ttcggtaccg gagcgtaaat tacgctcttg gccaccacca cgaatcagag gctctaacgt
     1621 caagccggtt ttgacaaata acaacccggt ctgttttggc ccgtaacact tagcggcatt
     1681 tagcgttagc aaatcaaccc ccatacgcgc cacattgaga tcaagctggc cggcggcctg
     1741 tgaaccgtca ctatgaaggt aaagcggagt cttgatgccg cgctgcagac gatcagcttt
     1801 gactcgtcgg acaagttcag cgacctcttt gataggctga acggtaccta gctcattatc
     1861 agccatagac agactcacta gtcgagtatg atcgtcgact tgccgttcga ttgaagcggc
     1921 actaactaaa ccgaacttat cagcctcggc cacccggcaa tcaaactgct cagctgctgc
     1981 tcggactgaa gcgtgttcgg tagcgccgac aattaggttg ccgccctggc ccatcataac
     2041 gccatggatg gccaaattga ttgattcggt agcaccggct gttaagatag cttcggtttc
     2101 tttggcgccg atgaccatag ctagacggtg ccgagcatcg tttaaagcgg atcgaacctg
     2161 acgagccgga ctgtaggcgg ccgaagggtt atagaactgc tccgtcaaat aagtctgcat
     2221 cacctgaaaa acccgctcat ctagcggggt ggcggcggca tggtcgaggt atgtttgatt
     2281 gaccatagac tatctatatt gtaacaggcg gatgcttctc cagtggcttt ccagtataag
     2341 ggtcacgttt gtagatttca cgcatcgtct gctcataata aagccggact gctaagtgga
     2401 gattcttaag ctcatcgcct tcaataaatt tataattctg accatcctga gc
//
LOCUS       DASSBW010000002         3887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_53001, whole genome shotgun sequence.
ACCESSION   DASSBW010000002 DASSBW010000000
VERSION     DASSBW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53001"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFG56_00020"
     CDS             <1..545
                     /locus_tag="VFG56_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622305.1"
                     /translation="FYLSIRRLFQDANLVHLKWWQRIIRSPMIETKTTFIVDLLGTSA
                     AIIGLVAIGIFAATGDARFDGLGGMVIGAAMMAGSLALIADVKGLIVGRSLSDHAAKQ
                     LTQRVRRLKGVHDVLDLRSMYIGSTKLLVVIEVHLDDKLGTDEIEALSDRIKLTAKKA
                     VPHAAVVQVEVETPDEELYT"
     gene            613..1593
                     /gene="cyoA"
                     /locus_tag="VFG56_00025"
     CDS             613..1593
                     /gene="cyoA"
                     /locus_tag="VFG56_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HET6622306.1"
                     /translation="MQKLYRNFLIVLLVLATVVVGWYIAGHNLPVLQPSGTVANQEKN
                     LIITIFVLMLIVAIPVFVLTGFIVWRYRENNTEAKYMPEYDSNQLAELTWWGIPIILI
                     TAIATLTWVSTDRLDPYKSLAVGVEPLKIQVVSLDWKWLFIYPESDLASINYAAIPVN
                     KPVEFDITSDSVMNSFWVPALGSQMYTMPGMKTKLNLMATETGNYYGSSANISGSGFA
                     RMNFTVAAMQPTDLTQWIDEVKNQHERPLDLSAYARLAKPSLPKARTAYSSVAPNLYD
                     KIVNQYMMPNMTIEPTVESPTHSSVDEPQQPVGDMPTGHIHMSMPMGDHQ"
     gene            1590..3578
                     /gene="cyoB"
                     /locus_tag="VFG56_00030"
     CDS             1590..3578
                     /gene="cyoB"
                     /locus_tag="VFG56_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002017509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HET6622307.1"
                     /translation="MIGQLAGLLLGRLGWDAIPTEPITLGAAISLPIMGLMVAGIITY
                     FKKWRWLWREWLTSLDPKKIGVMYLIVGGLMLLRGFADVLMMRAQQATSVGNSQGIVS
                     PDTFQQVFSAHGTIMIFFVAMGIMFGLINLLVPLMIGARDVAFPFLNATSFWLFTAGM
                     VLINLSLVIGEFSAAGWLAYPPLSETAFSPGVGVDYWIWSLQIAGVGSLLSGINFFVT
                     IIKMRAPGMTLMKMPIFIWSVLITMVMIMFAFPILTVTLTMLGLDRLMDMHFFTAGHG
                     GNMMMYVNLIWAWGHPEVYILVLPAFGIYSEIVATYSRKRLFGYTSMVVALAAIAFFS
                     FVVWLHHFFTMGAGSDVNAFFGITTMIIAIPTGVKIFNWLFTMFRGRIHLASPMIWFL
                     GFVFLFTTGGMTGVMLAVPAIDYQVHNSLFLVAHFHTMIISGVLFGFFAGITYWFPKI
                     FGFRLNETLGKMAAWSWIIGFVLAFFPLYVLGLMGATRRLDHYEISTGWQPLFIVAGI
                     GALIIALGIFFQVLQLVVSIWKRREYRVDNDPWNGRTLEWSLPSPVPFYNFAVTPTIA
                     RRDAFWEAKQIKKTKKPVYRPISLPKNSSLGLIIGGFSLIAGFAIIWHIWWLVVVGLI
                     GVVVTVIWRAADDETEYTLTAKQVEEYDKLHRTQEVYL"
     gene            3575..>3887
                     /locus_tag="VFG56_00035"
     CDS             3575..>3887
                     /locus_tag="VFG56_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622308.1"
                     /translation="MSRELAFANQEADDKTLFGFWVYLMTDCVLFGSIFATYAVLHNN
                     TYGGPGGGDLFYMPTVLAETIILLTSSFTCGMAVLAARMGRSRQVLAWLVATFLLGLS
                     FL"
BASE COUNT          880 a          910 c         1004 g         1093 t
ORIGIN      
        1 ctttttactt gagtatccga aggctgttcc aagatgctaa cttggttcac ttgaagtggt
       61 ggcaacggat tatccgctca cctatgattg agaccaagac gactttcatc gtcgacttac
      121 tgggcactag cgccgccatc attggtcttg tagctatcgg aatttttgct gccaccggtg
      181 atgctcgttt cgatggtcta ggcggcatgg ttatcggcgc ggctatgatg gccggttcac
      241 tggccttgat cgctgacgtc aaaggcttga ttgtcggtcg atcgttatca gatcatgcgg
      301 ccaaacaatt gacacagcgt gttcggcgtc tgaagggagt tcatgacgta cttgacctaa
      361 gatcgatgta catcggttcg actaagctac tcgttgtcat agaggttcat ctggatgaca
      421 aattgggtac cgatgagatt gaggcgttaa gcgacaggat caagctaaca gcaaaaaaag
      481 ccgtaccaca tgccgcggtc gtccaggtgg aagttgaaac accggacgaa gaactgtata
      541 cctaaaagtt aattgccagg ccttgcctag ctgctatact taaggttaag cggaagcttc
      601 ttaaggtgtg aaatgcaaaa actataccgt aactttttga tagtgttact agtactggcg
      661 accgtggtcg tcggttggta tatagctggc cacaatctgc cggtactgca gcctagcgga
      721 actgtggcta atcaagaaaa gaatctcata ataacgatat tcgtacttat gcttatcgta
      781 gcgataccgg tttttgtatt gactggtttt atcgtgtggc gctatcgaga gaacaacacc
      841 gaggccaagt acatgcctga gtatgacagt aatcaactag ccgaattgac gtggtggggt
      901 ataccgataa tcctaatcac ggccattgct acgctgacct gggtgagtac tgatcgtcta
      961 gacccctata agtcattagc agttggcgtt gagccgctta agatccaagt cgtatcgctt
     1021 gattggaaat ggctgttcat ttacccggaa agcgacttgg ccagcatcaa ttacgccgct
     1081 atcccggtca acaaaccggt tgagtttgat ataacgtcag attcggtgat gaattcgttt
     1141 tgggtgccag cactcggtag ccagatgtat accatgcccg gcatgaaaac caagctcaat
     1201 cttatggcaa ccgaaaccgg taactactat ggctccagcg ccaacatcag cggtagtggt
     1261 tttgctcgta tgaattttac ggtggccgcc atgcagccga ccgatttaac gcagtggata
     1321 gacgaggtca agaatcagca cgaaagaccg ttagatctct cggcgtacgc tcgtttagca
     1381 aaacccagct tgccgaaggc tagaacagct tacagctcgg tagcaccaaa tctatacgat
     1441 aaaatcgtca atcagtatat gatgccaaat atgactatcg aaccaacggt cgagtcgcca
     1501 actcattcgt cagtagacga accgcagcag cccgtcggtg acatgccgac tggtcacatt
     1561 catatgtcga tgccgatggg ggaccaccaa tgatcggaca attagccgga ctactactgg
     1621 gccgtttggg ctgggacgct attcccaccg aaccgatcac tctgggtgca gctatttcat
     1681 tgccgataat gggtctgatg gtggctggta tcatcactta cttcaagaag tggcgctggc
     1741 tttggcgcga gtggctgacc agcctagatc ctaagaagat cggcgtgatg tatttgatag
     1801 tgggcggact gatgctattg cgcggttttg ccgacgtgct catgatgcga gcacagcagg
     1861 caacatcggt aggtaactct caaggtattg ttagcccgga cactttccag caagtcttct
     1921 cggctcacgg tacaatcatg attttctttg tcgccatggg tatcatgttc ggtttgatca
     1981 acctgctggt accacttatg attggtgctc gtgacgtggc ttttcctttc ttaaacgcca
     2041 ctagcttttg gctgtttaca gccggtatgg tgttgatcaa cctatcttta gttatcggcg
     2101 aattttcagc tgctggttgg ctggcgtacc cgccactaag cgagacggct ttcagccctg
     2161 gagtaggcgt tgattattgg atatggtcat tgcaaattgc cggcgtcggt tcactactct
     2221 ccggcatcaa ctttttcgtc accatcatca agatgcgcgc tcctgggatg acgctgatga
     2281 agatgccgat cttcatatgg agtgtcctca taactatggt gatgatcatg ttcgcttttc
     2341 cgatattgac cgtcacactg actatgctgg ggctggatcg tctgatggac atgcacttct
     2401 ttacagctgg tcatggtggc aacatgatga tgtatgtcaa cttgatttgg gcgtggggcc
     2461 acccggaggt ttacatcttg gtactaccag cttttggtat ttattcggag atcgtggcta
     2521 cttattcacg taagcgattg tttggctaca cttcgatggt ggtggcctta gccgccatag
     2581 ccttcttctc ctttgtcgtc tggttgcacc acttcttcac catgggggcg gggtccgacg
     2641 tcaatgcctt cttcggcatc accactatga tcatcgctat tccaacaggt gtaaaaatct
     2701 ttaactggtt gtttactatg ttccgtggac ggatccatct ggctagccct atgatttggt
     2761 ttttaggttt tgttttcttg tttacgacag gtggtatgac cggtgtcatg ctggcggtac
     2821 cggctatcga ttaccaagtg cataacagtc tcttcctagt tgctcacttc cacacgatga
     2881 ttatctccgg cgtactattc ggcttctttg ccggcatcac atactggttc ccgaaaatat
     2941 tcggttttag acttaacgag acccttggca agatggcggc ttggtcttgg atcatcggct
     3001 tcgtcctagc cttcttcccg ctgtatgtac ttggattgat gggggcgacg cgccgactag
     3061 accactatga gatttcgact ggttggcaac cgttgtttat cgtggctggt atcggtgctc
     3121 tgatcattgc tcttggtatc ttcttccaag tactgcagct ggtggttagt atttggaaaa
     3181 ggcgtgagta tcgagtcgac aacgatccat ggaatggtcg gacattggaa tggtctctgc
     3241 caagcccagt tccgttttat aactttgccg ttacgccaac catcgctcga cgcgatgctt
     3301 tctgggaggc taagcagatc aaaaagacca aaaaaccagt atatcgacct atcagcttac
     3361 cgaaaaattc cagtttaggt ctaattatcg gtggattcag tctgattgcc ggctttgcta
     3421 tcatttggca tatctggtgg ttggttgtgg ttggcttgat tggcgtcgtc gtgacggtaa
     3481 tttggcgagc agccgatgac gagacggagt acaccctgac agccaagcaa gtagaggaat
     3541 acgataaact gcaccggacc caggaggttt atctatgagc cgtgaactag cttttgcaaa
     3601 ccaagaggct gacgacaaga cattgtttgg cttctgggta tatctgatga cggactgcgt
     3661 gctgtttggt agtatcttcg cgacttatgc cgttttgcac aacaacactt atggcgggcc
     3721 gggcggcggc gatcttttct atatgccgac cgtgctggct gagaccatca tcctgttaac
     3781 cagcagcttt acctgtggta tggcggtgtt agcggcccga atgggtcgat cacgccaggt
     3841 cctagcttgg ctagtggcta ccttcctgct aggactgagt ttcttaa
//
LOCUS       DASSBW010000003         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1008646, whole genome shotgun sequence.
ACCESSION   DASSBW010000003 DASSBW010000000
VERSION     DASSBW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008646"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1353
                     /locus_tag="VFG56_00040"
     CDS             82..1353
                     /locus_tag="VFG56_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HET6622309.1"
                     /translation="MILDKLKNSLPKKTDKSDNHIVALDIGTEFVKALIAELDGDNLT
                     VVGVGRARQDLSDMHGGAIADIGGVVRNCEAALAAAEEMAGLQAKKAVIGIAGELVKG
                     LTNTIRYKRPQPDRALDVSEMEFIIEKVQERAQAKAQQQIALETGNDEVEVKLVNSAI
                     VSIHIDGYKVSNPIGFQGKDVAIQIYTAFAPMVHIGALERTANELDLDLLAVSAEPFA
                     VSRSVLGTDASSTFTAILADVGGGTTDIAVVNDGGVEGTKMFGIGGRSFTRTIAGDMD
                     LSYQDAEKLKVNIEQPNIKESVKKQVDAAIDKTMDVWLGGVELALSEFDSVDHLPSKI
                     LLCGGGASLTKLVEAMQTSDWYKELAFTKRPAIQRIDPSEVIGIKDTTGEVNDHTYIT
                     AMGLLRVGYDTIVGNSADETVKDKLNRILRA"
     gene            1350..>2026
                     /locus_tag="VFG56_00045"
     CDS             1350..>2026
                     /locus_tag="VFG56_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622310.1"
                     /translation="MNKDVIYVDTEDDITSIIDKVKNSEAKVVALVPPKRVGVLQSVV
                     NLKLLTRAAKSADKQPVIITNNQALTNLAATAKLPVAKNLQSKPEMPEITALSVDDED
                     VINGEDLPVGEHAATAGAATGAVSLNQAVDELEEDEIGKTAAASAAAKAPKKSDKKSR
                     IPDFDSFRKRIFLLGGLGVLLVAFLVWAIFFAAHATVTITAKTIPYDINKVLTLSEDS
                     QLDPAQNV"
BASE COUNT          546 a          507 c          520 g          453 t
ORIGIN      
        1 cttcctcagc cggcatccgt cgtatcaaag ccgttttagt ttaacttaca taaccgcaat
       61 agggtataat taagcgtaaa tatgattctc gacaaattaa agaattcttt gcccaaaaaa
      121 accgataagt cagacaacca catcgtggct ctagacattg gtaccgagtt cgttaaagcc
      181 ctgatcgccg aacttgacgg tgacaatttg actgtggttg gcgttgggcg agctcgtcag
      241 gatttgtccg atatgcacgg tggtgctatc gccgatatcg gcggcgtagt caggaactgt
      301 gaagcggccc tggcggcggc cgaagaaatg gccggcctac aagccaagaa agccgttatc
      361 ggtatcgccg gcgagctggt caaaggtttg accaacacca tacgttacaa acggccacaa
      421 cctgatcgag ccctcgatgt atctgaaatg gaattcatca tagaaaaagt ccaggaacga
      481 gctcaagcca aagcccagca gcaaatcgcc ttggaaacgg gtaacgacga agtcgaagtc
      541 aaactagtca actcggccat cgtcagtatc catatcgacg gctacaaagt gtccaatcct
      601 atcggttttc agggcaaaga cgtcgctata cagatctaca ccgctttcgc cccgatggtc
      661 catatcggtg ccttagaacg gacagccaat gaactggacc ttgatcttct tgcggtctca
      721 gccgagccgt tcgcagtatc tcgctcggtg cttggcactg atgccagctc gaccttcacg
      781 gctattttag ccgatgtcgg cggtggcacg accgacatcg ccgtggtcaa tgatggtggt
      841 gtcgaaggga ccaagatgtt tggcataggt ggccgtagct tcacccgaac aatcgcaggc
      901 gacatggatc tttcctatca agacgcagag aagctgaagg tcaatatcga gcagcctaac
      961 atcaaggaat cggtcaagaa acaggtcgat gcggctatcg acaagaccat ggacgtctgg
     1021 ctggggggag tagagctagc cttgtcagag ttcgattcgg tcgaccactt gccgagtaaa
     1081 atcctactat gtggtggtgg cgcctccctg accaagttag tagaagccat gcagactagc
     1141 gactggtaca aggaactagc ctttactaag cgaccggcga ttcagcggat tgacccaagt
     1201 gaagtcatcg gcatcaagga caccaccggt gaagtcaacg accataccta catcactgcc
     1261 atgggcctgc ttcgagtcgg ctatgatacg atagtaggga attcagcaga tgaaaccgta
     1321 aaagacaaac taaatcgaat cttgcgcgca tgaacaaaga cgttatttat gtcgataccg
     1381 aagatgacat cacttcgata atcgataaag ttaagaactc cgaagccaaa gtcgtggccc
     1441 tggtcccgcc taagcgagtc ggtgttttgc agagtgtagt taacctgaaa ctactaactc
     1501 gagctgctaa atctgcggat aagcagcctg taattatcac caataaccag gctctgacca
     1561 acttggctgc aaccgccaag ctgccggtgg ctaagaattt acaatcaaag ccggagatgc
     1621 cggagatcac cgccttgagt gtagacgatg aagatgtgat caacggcgag gacttgccag
     1681 tcggtgagca cgccgctacc gccggtgccg ccacgggtgc cgttagcctg aatcaagccg
     1741 tcgatgaact agaagaagac gaaattggca agactgccgc cgctagtgct gccgccaaag
     1801 caccgaagaa gagtgacaag aaatcgcgaa taccggattt tgactcattt agaaaaagga
     1861 tttttcttct cggtggatta ggtgtcctgc tggtcgcttt cttggtttgg gctattttct
     1921 ttgctgctca cgctactgtc acgattacag ctaagactat tccgtatgac atcaacaagg
     1981 ttctgacttt gtcggaagat tcccagcttg atccagcgca gaatgt
//
LOCUS       DASSBW010000004         7183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1512850, whole genome shotgun sequence.
ACCESSION   DASSBW010000004 DASSBW010000000
VERSION     DASSBW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512850"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="serS"
                     /locus_tag="VFG56_00050"
     CDS             <1..1230
                     /gene="serS"
                     /locus_tag="VFG56_00050"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6622311.1"
                     /translation="YVRDNADIVQKNARNKGYDVDVRRLIDCDSERRGLQQQADELRE
                     RRNQNAGSMKGGKPTQALIDEGKQIKAELAETEAKLGKVNNAFQTLMDQFPNMVFDDV
                     PVGGEEDSVEIKKFGKQSSGATDHLDFANKRDWLDFERGVKVAGAKFYYLKGDLALLE
                     QALTSYALDFVVRRGFTFMTVPHMVNQRTMTGTGFAPRSSDQSDEYAIDGEDLSLIAT
                     AEIPLTGYHADEIIDEKDLPLRYAGLSPCYRKEAGTYGKHTRGLFRVHQFNKLEMYAF
                     TTPETSQDMHAELLNIEEELWQSLGIPYHVINIATGDLGAPAAKKYDIEYWSPVDEIY
                     RELTSCSNCTDFQARNLNIRVRRKDGSVEVLHTLNGTAVSLARSLVAVIEHYQTPDGK
                     LRVPDVLKSYMSGRDVL"
     gene            1269..1646
                     /gene="raiA"
                     /locus_tag="VFG56_00055"
     CDS             1269..1646
                     /gene="raiA"
                     /locus_tag="VFG56_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6622312.1"
                     /translation="MNKHIDITGIHYTPDDVTKRYVMKKIGGLDRFLNRHARKTMYAD
                     VKLRQVDRKKGNKYECDVILIIPDAKLNAKDSTMNMLAAIDIVEEKLKNQLKRYGEEK
                     SNHRGRHNLLSRVRNRAFRPKAY"
     gene            1731..4334
                     /gene="secA"
                     /locus_tag="VFG56_00060"
     CDS             1731..4334
                     /gene="secA"
                     /locus_tag="VFG56_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET6622313.1"
                     /translation="MKQHLTKVFGDPQARTIKRLEKRVKVVNELADKYRAMKTPELKK
                     QTDVLKKRLAKKGVKLDDILPDAFALVREAADRKLSMRHFDVQLVGGIALHEGNVAEM
                     KTGEGKTLVATAPVYLNALSGKGVHVVTVNDYLAQRDAGWMGQLYDFLGLTTGVIVND
                     QSFLVDPSYDNSDHDDPRMKLLRPVSRKEAYAADITYGTNNEFGFDYLRDNMVNDVDL
                     LRQRELNFAIVDEVDSILIDEARTPLIISAPASETPELYQKFAAVAKSLKPENYILDE
                     KRRSVSLSESGVDRVEKALGVKNFYNPENVRNVYHMDQALKAQALFKRDKDYVVTSDG
                     EVIIVDEFTGRLMHGRRYNEGLHQAIEAKESVTVQQESSTLATISFQNYFRLYDKLSG
                     MTGTAFTEAEEFQQIYGLDVIVVPSNRPLARQDKSDKIFKSEAGKVKALVADVKKFNE
                     EGRPVLIGTASITKNELISKALTKAKIPHELLNAKNNEREATIVAKAGEQGAVTLATN
                     IAGRGTDIVLGEGVKERGGLVVLGSERHEARRIDNQLRGRGGRQGDPGLTQFYVSTED
                     DLMRIFQGERIAALMDRLGVPDDEAIENKAVSKTLESAQKRVEGYHYDTRKNVVQYDN
                     VINRHRKVVYKVRREILEGENIKPEVDKLIEARLKELTVVPAKNNPKFKDELEAVIPL
                     GPKAIAKIGAEKNDKKRLELAVEAVSKLYVAKEKELGEEIMRGVEREVYLQVLDVLWM
                     QHLENMQHLREGIHWRSVGQRDPLVEYRSESQKLFESLQRTLQDEVLRAIMHVHLADI
                     NQQAVTDEKYDSELTQAAGNSVEQGVNEAGAPTTRGDQDFKVKKAQTKTTAKKKTKKK
                     RKAAKAARKRH"
     gene            4359..5639
                     /locus_tag="VFG56_00065"
     CDS             4359..5639
                     /locus_tag="VFG56_00065"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6622314.1"
                     /translation="MKHTVQEVELKNGARGLLIDVPGATVMSFQFHFRAGNRFARSKD
                     VEQTAHIMEHMAFGANAKYKDAHAYEAEFTRNGAYHNAYTTDFSMVYHGDCADFEWER
                     ILGLKQLAITKPRFNEEELEAERGNVKSELNGYLNDHHRLLWPKMMKNLGEDTLTFSE
                     ALKTVPSVSLKDVREHYRRTHTMSNMRFIIAGKMYRRKKRIIEMLESWDLPEGERLLI
                     PIDEQKKADPVLIRRKEASNLTFGWLMSLPRRLSDAELDAMSVVDHILNGTLHSRIYG
                     NARKRGLAYGVFSSTAGLEHNSEWEFGADVDLDTATQLIDIIVEQVSAMRRGNFTEEE
                     IEQAKQYALGRHQMGAQTVSQISRFYASRYFSDGWIEDYEARPQAIAAVTKKRVVDTV
                     SEFLNADCWIMGGVGNTDRAVMDGLQSRLETIFE"
     gene            5654..6964
                     /gene="murD"
                     /locus_tag="VFG56_00070"
     CDS             5654..6964
                     /gene="murD"
                     /locus_tag="VFG56_00070"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET6622315.1"
                     /translation="MKVAIAGYGIEGKASYQYWRSLGHQVTIVDERSLSAHDLPYGAA
                     SLTGPGSFKSLGGFDLVVRSPGVAPDRLKTDGKIWSATNEFLLKCRTPIIGVTGTKGK
                     GTTSSLIDAILTAAGKRTVLVGNIGRPALEAIEEASQADAVVFEMSSFQLWDVERSPH
                     VAVVLPIEPDHLDVHKNFKQYLSAKANIRRFQRADDICIYHSTNNYSADIAKSAGKQA
                     PSAARYGIADDGQVYVEDGNFCVRGQVICSTDNLRLPGVHNLDNACAAISAAWTLSVD
                     DQAVAAGLRSFAGLDHRLKLVGQADSVKYYDDSIATTPGSAIAAIAAFKQPKVLILGG
                     SDKGADYKELAEAVAASASMRAVLVIGQVGDKIEKSLKKAKVSSVVKRSAAKSMPEIV
                     QAAAGLAQPGDVVILSPAAASFDMFKSYADRGQQFVAQVETLSA"
     gene            complement(6903..>7183)
                     /locus_tag="VFG56_00075"
     CDS             complement(6903..>7183)
                     /locus_tag="VFG56_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622316.1"
                     /translation="NHFIEPDTTGWASLIENGKADQIDLTEITTAVRDGADVIVLACT
                     HYLALQDQLCRLAPKATIIEPTEAIAAQIKRLMSQLGRQTAGRDRHNF"
BASE COUNT         1888 a         1716 c         1948 g         1631 t
ORIGIN      
        1 tacgtccgag acaatgccga tatagttcaa aagaacgccc gtaacaaagg ctatgacgtt
       61 gatgtacgtc gtttgattga ttgcgacagt gagcgtcgcg gtttacagca gcaagccgat
      121 gaactacgcg aacggcgcaa ccaaaatgcc ggttcgatga agggtggcaa acccacgcag
      181 gctttgatag atgaaggtaa gcagatcaag gctgagttag ccgagactga ggctaagttg
      241 ggtaaagtaa ataacgcctt tcaaactttg atggaccaat ttccgaacat ggtatttgac
      301 gacgtaccgg tcggcggcga agaggatagt gtagagatca aaaaatttgg caagcaatcg
      361 tccggcgcta cggatcatct agattttgca aataagcgtg attggttgga cttcgaacga
      421 ggcgtcaagg tagctggggc gaagttctac tatctgaagg gcgatctggc cttgcttgag
      481 caggcactga ctagttatgc actcgatttc gtagtgaggc gcggctttac ttttatgacc
      541 gtgccacata tggtcaatca acggacaatg accgggaccg gctttgcgcc gcgcagctca
      601 gatcagtctg acgaatatgc tatcgacgga gaagatttga gcttgatcgc gacggctgaa
      661 ataccgctga ccggttatca tgccgatgag attatcgacg agaaggactt accgcttcgt
      721 tatgccggat taagtccgtg ttaccgcaaa gaagctggca cttacggcaa acacacccgt
      781 ggtttattcc gggttcatca gtttaataag ctagagatgt atgcctttac gactccagag
      841 acgagtcagg acatgcacgc tgaattgcta aacatagagg aagaactatg gcagagtcta
      901 ggtataccgt atcacgtcat caatatcgcc actggtgatc tcggcgctcc ggcggccaaa
      961 aaatacgata tcgagtattg gtcaccggtt gacgagattt atcgtgaact aaccagttgt
     1021 tctaactgca ccgactttca ggctcgtaac ttgaatatcc gagtccgccg caaggacggt
     1081 tcggtcgaag tgttgcatac cctaaatggc accgccgtca gtctggcgcg ttctttagtt
     1141 gctgttatcg agcactatca aacaccggac ggcaaactcc gagtgccgga cgtcttgaag
     1201 tcgtatatgt ccggccgcga cgtactttag atgatataat tagagtactt aaacgaaggg
     1261 ggactggtat gaacaagcat atcgacataa ccggaatcca ttacactcca gacgatgtca
     1321 ccaagcgcta tgtcatgaag aaaataggcg ggttagacag gttcctgaac cgccacgcgc
     1381 gcaaaactat gtatgccgac gtcaaattac gccaagtcga tcgtaaaaaa ggtaacaagt
     1441 atgagtgtga tgtaatcttg atcattcccg acgctaagct taacgccaag gattcgacta
     1501 tgaatatgtt ggctgctatc gatattgtag aagagaagct caaaaaccag ctaaagcgtt
     1561 atggagagga aaaatccaat caccgcggcc gccataacct actgtcgcga gtcagaaatc
     1621 gagcttttcg accgaaagcc tactagagta attgtttttg tatttaacct tagctttagt
     1681 tataatctta ggaagattgt aaaagccgat aggctagggg aagagacaaa atgaaacaac
     1741 acttaaccaa ggtgtttggc gatccgcaag cgcgcacgat caaacggctg gagaagcgcg
     1801 ttaaggtcgt caatgagcta gctgacaaat accgggctat gaagacgcct gagttaaaaa
     1861 aacaaaccga tgttttgaag aagcgcctag ctaaaaaagg cgttaagctt gatgacatcc
     1921 tgccagacgc tttcgcgttg gttcgtgagg cagctgatcg taaactcagc atgcgtcact
     1981 ttgatgttca gctagtcggc ggtatcgccc tgcacgaagg caacgtggct gagatgaaga
     2041 ctggtgaggg taaaactttg gtcgcaaccg cgccggttta cttaaatgcg ctcagtggta
     2101 agggtgttca tgtcgtcacc gttaacgact atctggccca gcgtgacgcc ggctggatgg
     2161 ggcagttgta tgacttctta ggacttacaa ccggtgtcat cgtcaacgat cagtccttct
     2221 tggttgaccc atcttatgac aactcggatc acgacgatcc gcgtatgaag ttgttgcgcc
     2281 cggtgtccag gaaagaagct tacgctgctg atatcacata cggcaccaac aacgagttcg
     2341 gcttcgacta tcttcgcgac aacatggtta acgatgtcga tttactcagg cagcgggagt
     2401 tgaactttgc catcgtcgat gaggtcgact ccatcctgat cgatgaagct cgaacaccgc
     2461 tgattatttc ggctccagct tctgaaacgc cggagcttta ccaaaagttt gccgccgtgg
     2521 ctaaaagtct gaagccggag aactacatcc tggacgagaa gcgacgcagc gtctcactat
     2581 ctgaatccgg tgtcgaccgg gtagagaagg cattgggagt caagaatttt tataatcccg
     2641 aaaacgttcg taacgtctac cacatggatc aggcactgaa ggctcaagcc ttgtttaaac
     2701 gcgataaaga ttatgtcgta actagcgacg gcgaagtgat tatcgtcgac gagtttaccg
     2761 gccgcttgat gcacggccgc cgctataacg aaggtctgca ccaggcgatt gaggccaaag
     2821 aaagtgttac ggttcaacag gaaagctcca ccttggcgac gatctctttc cagaattact
     2881 tccggttgta tgacaagttg tccggtatga cgggtacggc ttttaccgaa gctgaagaat
     2941 ttcagcaaat ttatggctta gatgtcatcg tggtaccgtc gaatcggcca ctagcccgcc
     3001 aagacaagtc ggacaagatc tttaagtccg aagctggcaa ggtcaaggcc ctggttgccg
     3061 atgtaaagaa gtttaacgaa gagggtcgtc cagttctgat cggtacggcc tcgattacta
     3121 agaacgaact tatctccaag gccttgacca aggccaaaat tccacacgaa ttgctcaacg
     3181 ccaagaataa tgagcgtgag gcaacgattg tggccaaagc cggtgaacaa ggtgccgtta
     3241 cgctagctac taacatagct ggtcgtggta ccgacattgt cttaggtgag ggcgtcaaag
     3301 aacgaggtgg tctggtagtg ctgggcagcg agaggcacga ggcccgtcga atcgacaacc
     3361 agctgcgtgg tcgcggtggt cgacaaggtg acccgggcct gactcagttt tacgtctcta
     3421 ccgaggatga cctgatgcgt atcttccagg gtgagcgaat tgctgcgttg atggatcgcc
     3481 taggagtgcc ggacgatgaa gcgatcgaga acaaggccgt gtctaagacg ctggaatcag
     3541 cccaaaaacg agtcgagggc tatcactacg acacccgtaa aaacgtggtt cagtacgata
     3601 acgtcatcaa ccgtcaccga aaagtcgtct ataaagttcg gcgcgagatc ctagaaggag
     3661 agaatatcaa accggaagtc gacaaattga ttgaagctcg acttaaagaa ctgaccgtgg
     3721 ttccggctaa gaacaatccg aaattcaagg atgaactaga agctgtcatc ccactggggc
     3781 ctaaggctat cgccaagatc ggcgccgaaa agaatgacaa gaagcgtctg gaattagccg
     3841 ttgaagccgt gagtaaactc tatgttgcaa aagaaaaaga gttaggcgag gagattatgc
     3901 gtggcgtaga acgcgaggtt tacctacagg tcttggacgt actatggatg caacacctcg
     3961 aaaatatgca gcatttgcgt gaaggtatcc actggaggtc tgttggccag cgcgacccat
     4021 tggtcgaata ccgctctgaa tcgcaaaaat tgttcgagag cttgcagcgg acgttgcagg
     4081 acgaagtcct gcgcgccatc atgcatgtcc atttagccga catcaaccag caggccgtga
     4141 ccgatgagaa atacgattcc gagctgaccc aagccgccgg taactcggtc gagcagggtg
     4201 tcaacgaagc tggcgctccg accactcgcg gtgatcagga ctttaaggtc aagaaggcac
     4261 agactaaaac aaccgctaag aagaagacca aaaagaagcg caaggctgcc aaagccgcca
     4321 ggaagcgcca ttaagcgata gaggatcgga agctagaggt gaaacataca gtacaggaag
     4381 tagagctcaa gaatggcgcg cgcggcctac tgatcgatgt tcccggcgct acggtcatga
     4441 gcttccagtt ccattttcga gccggtaacc gcttcgcgcg gtctaaggat gtcgagcaaa
     4501 ccgcccatat catggagcac atggcctttg gcgccaacgc taagtacaag gatgcccacg
     4561 cctacgaagc tgaatttacc agaaatggtg cttaccacaa tgcttatacc accgattttt
     4621 caatggtcta ccatggtgac tgcgctgatt ttgaatggga gcgtatcctc ggcctcaagc
     4681 agttggctat caccaagccc cgctttaacg aagaggagtt ggaggccgaa cgcggtaacg
     4741 tcaaaagtga acttaacggt tatttgaatg accatcaccg cctactctgg ccgaagatga
     4801 tgaagaatct tggcgaagat accctgactt tctcagaggc cctgaagacc gtacccagcg
     4861 tttccttaaa agacgttcgc gagcactatc gccgcaccca taccatgtcg aatatgcgtt
     4921 tcatcatcgc cggtaagatg tatcggcgca aaaaacggat tattgaaatg ctggagagct
     4981 gggatttgcc ggaaggcgaa cggttgctca tacctatcga cgagcaaaag aaagccgatc
     5041 cggtcttgat tcgccgcaag gaagcctcga acttaacttt cggctggctg atgagtctgc
     5101 cgcggcggtt atccgacgcc gaactcgacg ccatgtcagt cgttgaccat attctaaacg
     5161 gcacactgca ttctcggatt tacggcaacg cccgtaaaag aggcttagcc tacggtgttt
     5221 tttcatcaac tgccggccta gaacacaaca gcgaatggga attcggtgct gatgtcgact
     5281 tagataccgc cacgcagctc attgatatca tcgtcgagca ggtttcggct atgcgccgtg
     5341 gtaacttcac tgaagaagag atcgagcagg ccaaacaata cgccttgggt cgtcatcaga
     5401 tgggcgccca gactgtcagc cagattagcc ggttttatgc cagccgctat ttctcggacg
     5461 gttggattga agactatgaa gcgcgacctc aagccatcgc cgctgtcact aagaagcggg
     5521 ttgtcgatac ggtgagcgaa tttctaaatg ccgattgttg gatcatgggt ggtgtcggca
     5581 acaccgaccg ggctgtgatg gacggactac agtctcgact ggaaaccatc tttgaataaa
     5641 gtagaatagt cttatgaaag ttgctatcgc cggatacggc atagaaggca aggctagtta
     5701 tcagtactgg cgatcgcttg gtcatcaagt cacgattgtt gacgagcgat cattgtcggc
     5761 ccacgacctg ccatacggcg ccgcgtccct gactggaccg ggtagtttta agagcttagg
     5821 gggtttcgat ttggtggttc gatcgcctgg tgtggcaccg gatagactga agacggatgg
     5881 caagatatgg tcggctacca acgagttttt attaaaatgc cgcacaccaa tcattggtgt
     5941 taccggcact aagggtaagg gaactacatc cagcctgatc gacgccattc tgacggctgc
     6001 cggcaaaagg actgttctag tcggtaatat cggcagaccg gcattggagg ctatagaaga
     6061 agctagccaa gccgacgctg tggtatttga gatgagtagc ttccagttgt gggatgtcga
     6121 gcggtcaccg cacgtcgccg tggtattgcc gatagagccc gaccacttgg atgtacataa
     6181 gaattttaag cagtatttga gtgccaaagc caatatccgt cgcttccagc gagctgatga
     6241 tatctgcatc taccattcta ccaataatta ttcggccgat attgccaaat cggccggcaa
     6301 acaagctccg tcggccgctc gttacggtat cgcagacgac ggtcaggtat acgtagagga
     6361 cggcaacttc tgcgtccgtg gtcaagtgat ttgttcaacc gataatcttc gtttgccggg
     6421 cgtacacaat ttagacaatg cctgtgccgc tatcagcgct gcctggacgc tatccgtcga
     6481 cgaccaggcg gtggctgctg gcttgagatc gtttgccgga ctcgaccatc gactcaagct
     6541 ggtaggccag gccgatagcg ttaagtacta tgacgactcg attgccacga ctcctggctc
     6601 tgccatcgcg gctatcgctg cttttaagca acccaaagtc ttgatactgg gtggctccga
     6661 caaaggcgcc gactataagg aattagccga agcggtggcg gctagcgcat cgatgcgcgc
     6721 agtgctggtc atcggccagg tgggagacaa gatcgaaaaa tctctcaaaa aagccaaggt
     6781 cagtagcgta gttaagcggt cggcggctaa gtctatgccg gaaatagtcc aggccgccgc
     6841 cggtttggct caacctggtg acgtggtgat tttgagcccg gcggcagcta gctttgatat
     6901 gttcaaaagt tatgccgatc gcggccagca gtttgtcgcc caagttgaga cattaagcgc
     6961 ttgatttgag ccgcgattgc ttccgtcggt tcgatgattg tggctttcgg tgccaaccgg
     7021 cacagttggt cttggagcgc cagatagtgg gtgcaggcca agacgatgac gtcggcgccg
     7081 tcacggacgg cggttgtaat ctcggttagg tctatttgat cggctttacc gttttcaatc
     7141 agactagccc agccggtagt gtcgggttcg ataaaatgat tat
//
LOCUS       DASSBW010000005         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_308709, whole genome shotgun sequence.
ACCESSION   DASSBW010000005 DASSBW010000000
VERSION     DASSBW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308709"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFG56_00080"
     CDS             complement(<1..569)
                     /locus_tag="VFG56_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622317.1"
                     /translation="MELLKLATRRSGWSELLYVALNLAYAAALLTLVLLFNPPWLAYL
                     VVVLSKWRTFAVRPRYWFANLQANLVDALVVGSFVTLMWRADTNLWLQIVLAVLLAVW
                     LTFIKPQSKRQWVLIQAMVCQAISLTALFSLAYHTVASLVVITAWLIGYTAARHALSS
                     YNEESERTLLSLAWSLLIAELSWLGYHWT"
     gene            complement(556..2172)
                     /locus_tag="VFG56_00085"
     CDS             complement(556..2172)
                     /locus_tag="VFG56_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET6622318.1"
                     /translation="MDKRPRKSIDGMLLKRREDSPLSSGQSSNDTGLPNRFIDQSRTT
                     QPAEQALPQPTRLNRSEIDASLDSIDQAGAVQPRAKQPFRRPHRLALPKITKKRVVLA
                     LVLILLLFAGFFGYKIWNASSKVLGGGNIFDLLGGGEQLQTGQYGRSNILVFGTSRDD
                     PGHSGAYLTDSMMVLSLDQDKKNAIMFSVPRDLWVKYDTACAAGYEGKVNALYQCYSN
                     DGQRHEKGAAALRQKIGQVFGIDVQYSVAVDYTAVVDLVNAVGGIDITIDSPDPRGIL
                     DREFDRKCPNGPYTCYRVKYDNGPAHLNGKQALYLARARNAHTGYGLPRGNFDREANQ
                     RKILQAIRDKAISAGTLANPIKVSDILDALGDNIRTNFTAGETKTLIGLAQDIQDKDI
                     RNISLVEPPHEVVTTGSVGAQSVVKPAAGLYDYSGIQNYIKGQFSFSPLTQENASIEV
                     LNGGASSGAAGREADKLTEAGFVNVTTGDTPTEQTYQALQWYDLSDGSKSQTADKLSQ
                     ALGQAASGSQLPKGVTSEADFVIILSDGAS"
     gene            2240..2845
                     /gene="lepB"
                     /locus_tag="VFG56_00090"
     CDS             2240..2845
                     /gene="lepB"
                     /locus_tag="VFG56_00090"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6622319.1"
                     /translation="MKINFLERHPVIKDLAGIVVFFAAVIIGAWLINSFVFRSFSVSG
                     PSMEPTLYTGDRLIVNRLPITGDRLLGRTYVPERGQVIVFKNPHFEPGMQDEFIVKRV
                     IGLPGERVVVKDGQMTVYNSAQPDGFNPDKLVDGADGPTSPTFGNVNTVVPPSQLFVA
                     GDHRQEGYSLDSRDGLGTIPLDDVIGPVAFRIYPFDRARSF"
     gene            complement(2842..>3049)
                     /locus_tag="VFG56_00095"
     CDS             complement(2842..>3049)
                     /locus_tag="VFG56_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622320.1"
                     /translation="IGHKKGERTVAKRNSGLKHTLTETDFTKTLAGRLDLPVRQKTTA
                     KGGQIIISFKDEQDLDLIKNKLGF"
BASE COUNT          710 a          815 c          783 g          741 t
ORIGIN      
        1 atcgtccagt gatagcctaa ccagctcagt tcggcaatca aaagactcca cgccagactc
       61 agaagcgttc gttcactctc ttcattgtaa gagctgaggg cgtgccgggc ggcggtatag
      121 ccgatcagcc aagccgttat caccactagc gaagctacgg tatggtaagc caaactaaat
      181 aaagcggtca aactgatagc ctgacaaacc atggcttgaa tcaacaccca ttgtcgctta
      241 gactgcggtt tgataaaggt cagccaaact gccagcagca cagccaggac gatctgcagc
      301 cataagttgg tgtcggcccg ccacatcagc gtgacaaagc taccgacaac cagggcatca
      361 accaggttgg cttgcaggtt ggcgaaccaa tagcgcggcc gcaccgcaaa agtccgccat
      421 ttactgagga ctacgaccaa gtaggctaac cacggcggat taaaaagcag aaccagagtg
      481 agtaaagcag cagcgtacgc caggttaagg gcgacgtaga gtaattcact ccagcccgac
      541 cggcgggtgg ctagtttaag aagctccatc gctaagtatt ataacaaagt cagcttctga
      601 ggtgacgccc tttggtaatt gactaccgct cgcggcttga cctagggcct gagagagttt
      661 atcggcagtt tgcgacttgc tgccatctga caagtcatac cattgcagcg cctgataggt
      721 ctgctcggtc ggtgtatcac cagtagtcac gttgacgaag ccggcttcgg tcagcttgtc
      781 ggcttcacgc ccggccgcac cagagctagc gccgccattg agaacttcga tgctggcatt
      841 ttcctgggtt agtgggctga aactaaactg acccttgata tagttttgga tcccgctgta
      901 gtcatataga ccggccgccg gcttgaccac gctttgagca ccgacactac cggtagtaac
      961 tacctcgtgt ggtggctcaa ctaggctgat attgcggata tctttgtctt gaatatcttg
     1021 ggctaaaccg ataagggtct tggtctcacc ggctgtgaag ttagttcgga tattgtcgcc
     1081 tagagcatca aggatgtcac tgaccttgat tgggttcgct agagtaccgg cgctaatcgc
     1141 tttgtcccta atggcctgaa ggatcttgcg ctgattggct tcgcgatcga aattaccgcg
     1201 tggcaagcca tagccggtat gggcgttacg ggctcgcgcc aggtacaagg cctgtttgcc
     1261 gttcaagtgg gccggtccat tgtcgtattt tacgcggtaa caagtgtagg gtccgttagg
     1321 gcatttgcgg tcgaattccc tgtccaagat accgcgagga tcgggactgt cgatcgtaat
     1381 atcgataccg ccaacggcat tgaccaaatc aaccaccgcc gtgtagtcaa ccgccaccga
     1441 atactgcaca tcgataccga agacttgacc gattttttgt ctgagcgccg cagcaccctt
     1501 ttcgtgacgc tggccatcat ttgagtagca ctgatatagc gcattgacct taccctcata
     1561 accagcggca caagcggtgt cgtatttgac ccataagtcg cgcggcacgc tgaacatgat
     1621 ggcattcttc ttgtcttgat ccaggctcaa caccatcatc gaatcggtca ggtaagcgcc
     1681 actatggccc gggtcgtcgc gggacgtacc gaagaccaag atgttagacc gtccgtactg
     1741 gccggtctgc agctgctcgc caccgcccag tagatcaaag atattgccgc cacccaggac
     1801 cttggagcta gcgttccaaa tcttgtagcc aaaaaagcca gcgaaaagta acaatataag
     1861 tactagggct aacaccaccc gttttttggt aatcttcggt aaagcaagac gatgcggacg
     1921 gcgaaacggc tgtttagctc gtggttgcac ggccccggct tgatcgatgg aatcaagcga
     1981 agcatcgatc tcgctgcggt ttaaccgagt tggctgcggc aaggcttgtt cagctggctg
     2041 tgtcgtcctg gattgatcga taaagcggtt tggtaaaccg gtgtcattag acgattggcc
     2101 gctgctgagc ggactatcct cacggcgctt taaaagcatg ccgtcaatac ttttgcgtgg
     2161 tcttttatcc atttaacttg agttcaatta tagcggcttc tgcgcttcgc gtaaacaaaa
     2221 tctgttatgc taatagttga tgaaaattaa ctttcttgag cggcatccgg tgatcaaaga
     2281 cctagccggt atagttgtct tttttgccgc cgtcattatc ggcgcgtggt tgattaactc
     2341 cttcgttttc cgcagtttct cggtatccgg acctagcatg gaaccgaccc tttataccgg
     2401 tgatcggctg atcgtcaacc ggctaccgat aaccggcgac cgcctactcg gccgtactta
     2461 cgtaccggaa cgtggccaag tcatcgtctt taaaaatccg cacttcgaac ccggtatgca
     2521 ggacgagttt attgtcaaac gtgtcatcgg cttacccggc gaacgcgtgg tagtaaagga
     2581 cggccagatg accgtctaca atagcgctca gcccgacggc ttcaatcccg acaaactagt
     2641 agatggcgcc gatggcccga ctagtccgac ttttggtaac gtcaacaccg tcgtaccgcc
     2701 cagtcagtta tttgtcgctg gagaccatcg ccaagaaggc tattcactcg attcgcgcga
     2761 cggcttagga acgatcccgc tcgacgatgt catcggtccg gtagctttta gaatctaccc
     2821 ctttgaccga gcccgcagtt tctaaaaacc cagcttattc ttgattaggt ctaaatcttg
     2881 ttcgtctttg aaactgataa ttatctggcc acccttggcg gtagtcttct gtctgactgg
     2941 caaatcaagt cgaccagcta aggtcttggt gaaatcagtc tcagtcaagg tgtgcttgag
     3001 acccgagtta cgcttggcca ctgtccgctc acccttcttg tggccgata
//
LOCUS       DASSBW010000006         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_792600, whole genome shotgun sequence.
ACCESSION   DASSBW010000006 DASSBW010000000
VERSION     DASSBW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792600"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFG56_00100"
     CDS             complement(<1..345)
                     /locus_tag="VFG56_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622321.1"
                     /translation="MLAIILAALTIVSTVAGGVTAYKNRDRLHRLLGFTAGVIIGVVA
                     FDLLPEIFNHLQNDNDHMTAAMLALVGGFLLFHIVEKTILIHHAQEGYYEDHHHPTVG
                     LVNGLALAGHSLL"
     gene            complement(345..1775)
                     /gene="zwf"
                     /locus_tag="VFG56_00105"
     CDS             complement(345..1775)
                     /gene="zwf"
                     /locus_tag="VFG56_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6622322.1"
                     /translation="MDSTDFNIKLNPAVIVIFGITGDLSTRKLLPALYQLTRNGLLHE
                     KTRVVGVTRRQVELDSLLDRAKVCVRDKDGECDEQALGELTKRLELYQMNAAEADGYS
                     GLQAYLDKLEDEVGMCVSRLFYLSIPPQISTPIIRHLGQQGLNQGCKKHDAKARLLLE
                     KPFGFDMRTAEELVAETTKYFDESQIFRIDHYLAKETVQNIVTFRFRNPIFEGIWNNR
                     HISSIEVVADEKLDIEGRANFYEQTGALRDLIQSHLMHVMSVVMMDKPADITDADAMH
                     SSRLALLNSIEAVPVDRLDRRAIRGQYDGYRQEVDNDDSYVETYAALKLFSRLETWRG
                     VPIYLRTGKALASKQTAVTISFKPEAADHDHINQLTFNIQPNEGIQIQLWVKRPGFER
                     RLQTAPMSFRYDQTFDEHGHPDAYERVLVDAIRGDHMLFATSDEVLSAWRIIEPVITN
                     WAQDSNSLKAYTKASAGPDLSKMEED"
     gene            complement(1775..2677)
                     /locus_tag="VFG56_00110"
     CDS             complement(1775..2677)
                     /locus_tag="VFG56_00110"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET6622323.1"
                     /translation="MKITIVGLGKMGSQIAERLVKSGHSVVAVDPSLAAIKAAEAFGA
                     GSADSRQDVAEKFADEPVAVWLMIPAQFVSAEVEAWLEVLPEGSLLIDGGNTDFRETR
                     RHAELCGAKGVNFVDVGVSGGVLGAQAGFSMMVGGDQAGYERIKPILEALAQPSGGYD
                     YFGSAGAGHYVKMVHNAVEYGMMEALAEGYQILKEGPYDNLDLAKAGAVWQKASVIES
                     TLNQLVAEVMVENPSQDGVSGYVAESGEARWTLEVAKETGIELPVIKTALGVRIRSGQ
                     GEVSYATKVLASLRNKFGGHKINK"
     gene            complement(2674..3345)
                     /locus_tag="VFG56_00115"
     CDS             complement(2674..3345)
                     /locus_tag="VFG56_00115"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET6622324.1"
                     /translation="MKFIKKSKGLAEVIADALTAALKRHDRVVWLVSGGSNVPISVQA
                     MKLIDEDLSAKLVVMQVDERFVEADSPDSNWHQLMKAGFEPKRASVYPVLAHEYIDVE
                     QAARAYEQVAKDQFSQSDFIIAQLGVGDDGHTAGILPGSAAASALELVTGYAAERFER
                     ITLTFPALELVDEAYVFAYGGSKLYALTRLMNNHLPLETLPATILRRLAQAVVYNDQI
                     EGETK"
     gene            3425..>3670
                     /locus_tag="VFG56_00120"
     CDS             3425..>3670
                     /locus_tag="VFG56_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622325.1"
                     /translation="MDDEPYQEVYFIGITLPTDLDRQVAKLKNRLYQADTELLQPVLP
                     HVTLLHPPSLSGILPSELIPKVRQVAERYLPLTVALND"
BASE COUNT          873 a         1019 c          847 g          931 t
ORIGIN      
        1 gagtaagctg tggccagcta gagctaagcc gttgactagg ccgaccgtcg ggtggtgatg
       61 atcttcgtaa taaccttctt gagcatggtg gatcaagata gtcttctcga cgatatgaaa
      121 tagcaaaaaa ccgccaacca aagccagcat ggcagctgtc atatggtcgt tgtcgttttg
      181 caggtggttg aagatctccg gcaataaatc aaacgccacc acaccgatga tgacaccggc
      241 cgtaaagcca agcagtctgt gcaatcggtc acgattcttg taggcagtga caccacccgc
      301 tacggtcgat acaatcgtca aggccgctaa gattatggct agcatcagtc ctcctccatt
      361 tttgataggt caggaccggc cgaggctttg gtgtaagctt ttagactatt gctgtcttgt
      421 gcccagttcg taattaccgg ctcgatgatt cgccaagcgg agagcacctc gtcgctagtg
      481 gcaaacagca tatgatcacc gcgtatggca tcgaccagca ccctttcgta agcatccggg
      541 tgtccgtgct catcgaaagt ctgatcgtaa cggaagctca tcggtgccgt ctgtaaccgc
      601 cgttcaaaac ctggtcgctt gacccataac tgtatctgaa taccctcatt gggctgaata
      661 ttaaacgtca gttggttgat atgatcatga tctgcggctt ctggcttaaa gctgatagtg
      721 acagccgtct gcttggaggc cagggctttg cctgtcctga gatatattgg tacgcctcgc
      781 catgtttcca gtcgcgagaa aagcttgagt gccgcgtaag tctcgacgta cgaatcatca
      841 ttgtcgactt cttggcgata gccgtcatac tgccccctta tcgctcgtct gtccagacga
      901 tctaccggca ctgcttcgat cgaattcaat aatgccagcc ggctactgtg catagcatcg
      961 gcatcagtga tatcagccgg tttgtccatc atgacaactg acatgacatg catcaaatgg
     1021 ctctgaatca aatcccgtag tgcaccggtc tgctcgtaaa aattagcccg gccttctatg
     1081 tccagtttct catcggccac cacctcgatc gaactgatgt ggcggttatt ccatatacct
     1141 tcaaagatag ggttacgaaa gcgaaaagta acgatgttct ggacggtttc tttagccaga
     1201 tagtgatcga ttcgaaagat ctgcgactca tcaaagtatt tggtcgtctc agccaccagc
     1261 tcctcggcgg tacgcatgtc aaagccaaat ggcttttcta gtagcagccg ggctttagca
     1321 tcgtgcttct tgcaaccctg gtttaaacct tgctggccca aatgtctgat tatcggtgtc
     1381 gatatttgag gcggaatcga taagtaaaac agacgactga cacacatgcc gacttcgtct
     1441 tccaacttat caagataggc ctgcaaaccc gagtaaccat cggcctcggc ggcattcatc
     1501 tgataaagct caagtcgctt tgtcagctca cctagggcct gctcatcaca ctcaccatct
     1561 ttgtcacgaa cgcagacctt ggctcggtca agcaaactat ctagttcgac ttgtcggcga
     1621 gttacaccta ctacccgggt cttttcatgt agtaagccgt ttctggtcaa ctggtaaaga
     1681 gccggtaata atttgcgtgt cgataaatca ccggttatgc caaaaataac gataaccgcc
     1741 ggattgagct tgatattaaa gtcggtgctg tccattattt attgatttta tgtccgccaa
     1801 atttatttcg aagtgaggcc aatactttag tagcgtaact gacttcaccc tgccctgatc
     1861 ttatacgtac ccctagagct gttttgatga ctggtaactc tataccggtc tctttggcga
     1921 cctctagcgt ccagcgggcc tcgcctgact cggcgacata accactgacg ccatcttgac
     1981 tggggttttc aaccatcact tcggctacca gttggttgag cgtactttcg ataacgctag
     2041 ccttctgcca aaccgctcca gctttggcca agtccagatt gtcatacgga ccttccttga
     2101 ggatttgata accctcggcc aaagcctcca tcatgccgta ttcgacggca ttgtggacca
     2161 ttttgacata gtgaccagca ccagctgatc caaaataatc atagccgcca ctcggctgag
     2221 ccaatgcttc aagaatcggc ttgatccgct cataacctgc ctggtcgcca ccaaccatca
     2281 tcgagaaacc ggcttgcgca cccaatacac caccactgac accgacatcg acgaaattaa
     2341 caccttttgc accgcatagc tcagcgtgtc gcctagtttc ccgaaaatcg gtattgccgc
     2401 catcgattaa caagctgccc tcgggtaaga cctctagcca ggcttcaact tcggccgaga
     2461 cgaactgggc cggtatcatc agccagaccg cgaccggttc gtctgcaaac ttttcggcta
     2521 catcctgtcg gctgtcggcc gagccagcac caaaagcttc agccgctttg atggcagcta
     2581 gactggggtc taccgccacg accgagtggc cggatttgac cagccgctcg gcaatttgcg
     2641 agcccatctt ccctagtccg acaatcgtta ttttcatttt gtttcgcctt ctatctgatc
     2701 attataaact accgcctgag ccaaacgccg taagatagta gctggcagag tctctagcgg
     2761 taagtgattg ttcatcaatc tggtcaaagc atatagctta gagccaccat aagcgaaaac
     2821 gtaggcctcg tcgactaatt ctagtgcggg aaaggtcagt gtaatccgct cgaagcgctc
     2881 ggcggcataa cccgtcacaa gttccagagc actcgcagca gcactgcctg gtaagatacc
     2941 ggcggtgtgt ccatcgtcac cgacacccag ttgggcgatg atgaaatcag attgactgaa
     3001 ctggtccttg gcgacctgtt cgtatgcccg tgccgcttgc tctacgtcga tgtattcgtg
     3061 cgccaacacc ggataaacgc tggcccgttt aggttcgaaa cctgccttca tcagttggtg
     3121 ccagttcgaa tccgggctat cggcttcaac aaagcgctca tcgacttgca taacgaccag
     3181 cttggcgctt aggtcttcgt cgattagctt catcgcttga acgctaatcg gcacattgct
     3241 gccacccgac accaaccaga cgacgcggtc gtgccgtttc aaagcggccg tcagtgcgtc
     3301 ggctataacc tcggctagtc ccttactttt cttgataaac ttcattccat aaccagtatg
     3361 acatggtgca agctcaacgt cgattatctg acacggtata gccataacag ctaaaatagt
     3421 aggtatggac gatgaaccat accaggaagt ttattttatc ggtatcactc tgccgactga
     3481 tcttgatcga caggtcgcta agctcaagaa ccgtctctat caggctgaca ccgagctgct
     3541 tcagccagtc ttacctcatg tcaccttact ccatccgccc agccttagcg gtatccttcc
     3601 gagcgagttg ataccaaaag ttcgccaagt cgccgagcgt tacttgccgc taaccgtcgc
     3661 tttgaacgac
//
LOCUS       DASSBW010000007         7023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_146939, whole genome shotgun sequence.
ACCESSION   DASSBW010000007 DASSBW010000000
VERSION     DASSBW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146939"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFG56_00125"
     CDS             <1..547
                     /locus_tag="VFG56_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622326.1"
                     /translation="NGTVDWPANLKISGNVDLTNKCKVIVHGNIWITGSLSLSNSSQL
                     IVASGLATPPTIMVDGAGGVVATNGSSFASNSGDIGFLVITYWSKASCSPDCSNVSGS
                     DLFDSSQVTTIQLSNSSTGPNTEFYARWSQVELANAGNIGALVGQEVRLTNSSAVTFG
                     TEVAGAGGIIGWVVDSYKRAY"
     gene            597..1649
                     /gene="pilM"
                     /locus_tag="VFG56_00130"
     CDS             597..1649
                     /gene="pilM"
                     /locus_tag="VFG56_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET6622327.1"
                     /translation="MTKLFHHDKPIIGLDISQTGLRLMALDAKKWKVHGYGSLNLDPL
                     KLQDGLNKGDDYLAKNLKVMLKKNILGHLPSNQVAVSVPTSKTYSRTLNLPLDAENNI
                     NEAVRLEADQYIPVPVDELNIDYEIIERNKEGLSVLISATPKKIVENIVTSLETAGLE
                     VVFAEPGVSSIARLLRHTEDGNLPTIIVDIGAASTDIAIFNKTIRVSGGVAIGGNSLT
                     IAISKKLDISLEEAHKIKVLDGLSTGPKRTKLEEAMEPSLNNICDELKKMTRYYTDRL
                     EMKDKIEQIIMSGAGSNVPGMADYFTDKLETAVRVASPWQKLKFGKLPQPSKQNKPRF
                     ISATGLAFVTPEDVWK"
     gene            1646..2248
                     /locus_tag="VFG56_00135"
     CDS             1646..2248
                     /locus_tag="VFG56_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622328.1"
                     /translation="MINLLSSEHKRNLRAARVNVTLNRYVNLIILSAVLAGVIFGAGY
                     FLTMHERSQAEAGIADNKQQIAKYDKIRRQAEQFETNLRSARVILGGQVTFSQLIVDI
                     AKTLPEGAVLQSLHIETEDIGGKPITLSARTIDAGITPLTLKSALEQSPLFSEVSIIS
                     IKRNLVTGEQRVETSQSDYPVSVNLSVSLDKTAGKPGDKS"
     gene            2245..2832
                     /locus_tag="VFG56_00140"
     CDS             2245..2832
                     /locus_tag="VFG56_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622329.1"
                     /translation="MKKGQLTAVQLMYGLYGLIAVLLIATGVGFYYAKNFLGSAVADT
                     NDLKDQASVSQQNVVKLQQLQNELHSQADVVERTRQIMADSQHYQYQDQIVKDINTYA
                     NRTGVQVIGYDFGDPTKKSANKSSLPTVSGTRIITADLTLDAPVKYTRFLNFLQAIEQ
                     NLTKMQVTGVNITPDEQDADYVSNPGISLQIYVKN"
     gene            2835..3143
                     /locus_tag="VFG56_00145"
     CDS             2835..3143
                     /locus_tag="VFG56_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622330.1"
                     /translation="MSNKTTTTSSKQLTAQLKAAGQPIAKHHVVIVVVLFLGFLTYAV
                     FTVSQILLSNDQSMDTKAQEQAITTNFDQKTIEQIQQLKSRQQSTDLSLPEGHINPFD
                     "
     gene            3314..3889
                     /locus_tag="VFG56_00150"
     CDS             3314..3889
                     /locus_tag="VFG56_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622331.1"
                     /translation="MLLPIERFLGAPLLSLQLEAKIASLAEPIIDPRQLKLVAFYVDS
                     PKSVDEDLVIFSSDIREVNQQLGMLIDNDDVITPLDDLVRLNEIIEFNFSLPGVKVVD
                     ERGRKYGKVTSYNLDPNSFFVEQLVVEPSIWNNLNNANNLVHRNQIVSVTNKRIVIKA
                     PTVKAKRETRQTTDFVNPFKELKQPESSRPA"
     gene            complement(4201..4845)
                     /locus_tag="VFG56_00155"
     CDS             complement(4201..4845)
                     /locus_tag="VFG56_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET6622332.1"
                     /translation="MKITAITAQIQDKNRVNVSLDGKYRFSLDVLQVTELDIRVGRDI
                     GEEELVQLEEESQFGKLYTRALEYCMMRPHSVKEVRDYLRRKTLAKRYKSKKTGELKE
                     RPGVSQSIADRVLERLLDKGYLNDESFTRYWLENRSLSKGASRRKLVAELRSKGVDRA
                     IIDQQLEVTERSDNDEIKKIIAKKRSRYPDEQKFIAYLARQGFSYDDIKNALSD"
     gene            complement(4860..5894)
                     /gene="recA"
                     /locus_tag="VFG56_00160"
     CDS             complement(4860..5894)
                     /gene="recA"
                     /locus_tag="VFG56_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET6622333.1"
                     /translation="MADKKTKTAAKNDAAKDGKSQALGLALEHITKAYGDGSIMKLGD
                     AGKVNVATTPSGALSLDLALGGGYPKGRIIEIYGPESSGKTTLTLHAIAEIQKNGGTA
                     AFIDAEHALDPAYAKRLGVDTDNLLVSQPDNGEQALEIAETLVRSNAVDLVVVDSVAA
                     LVPQAEIEGDMGDSHMGLQARLMSQALRKLTGAINKSKTTVIFINQIRMKIGVMFGNP
                     ETTTGGNALKFYSSVRLDIRRIGQIKVGEDVIGNRAKVKVVKNKIAPPFRVAEFDIMY
                     NQGISKTGDVLDLAVVHGIVGKSGAWFDYKDGKIGQGREAAKEYLRENPKVLAEIDKA
                     VRAKVAEADV"
     gene            5947..6348
                     /locus_tag="VFG56_00165"
     CDS             5947..6348
                     /locus_tag="VFG56_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622334.1"
                     /translation="MRKAQGFTVVEVILVIVVLVAAGLIFWHEKSVVDAKNRDLTRKT
                     AINAIAYSLDNVYHVKHGYYPDSLNDKTFMVIDPAIMRDPRGHAVGDKDSDYRYEPTN
                     CAGGQCQGYSLRAQLEYEADYVKSSDDHASD"
     gene            complement(6345..>7023)
                     /locus_tag="VFG56_00170"
     CDS             complement(6345..>7023)
                     /locus_tag="VFG56_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622335.1"
                     /translation="YDTEGRPLYLHPAETAPAQPQVVHASRPLEPPKPEVSAEIAKKH
                     EASKKAYPQLNLSEGEYVITVIKRHIIGLLSIWATVTILILAVCVLVPAILWNNGIMR
                     EAGLLGIGAVFALGLLFLIGGYISAYVYNSNQLFLTNEGIVQQVRLSLFAQRTQSVSL
                     GSVEDVSFFKSGIVQYIFNYGKIRLSTVGDENTYRLTYVGHPALQVKLLNDAVEAYKN
                     GRPVGNH"
BASE COUNT         1892 a         1828 c         1593 g         1710 t
ORIGIN      
        1 caatggcacg gtcgactggc cagccaactt aaagatcagt ggtaacgttg atctgaccaa
       61 taaatgtaag gttatcgtcc atggaaacat ctggataacc ggtagcttat cgcttagtaa
      121 ctcgtcacaa ctcatcgtag ccagtggttt ggctacacca ccgacgatta tggtcgatgg
      181 agctggcggg gtcgtcgcta ctaacggttc gtcattcgcc tcaaatagtg gtgacatcgg
      241 ctttttagta atcacctact ggtcaaaagc cagttgttca ccggactgca gcaacgtcag
      301 tggtagcgat ttgtttgaca gtagccaagt taccacgatc cagctatcca acagctcgac
      361 cggacctaat accgaattct acgctcgctg gagccaagtt gaactagcca acgccggcaa
      421 tatcggtgct ctcgtcgggc aagaagtcag attaactaac tcatcggccg tcacattcgg
      481 taccgaagtg gcgggtgccg ggggtatcat tggctgggta gtcgattctt ataagcgagc
      541 ctactagagg tggtggtgtg ttataatcag cttaagctta cagggtgcgg catcaaatga
      601 caaaactatt tcatcacgac aaaccgatca tcggtctaga catctctcag accggtctta
      661 ggctaatggc cttggatgcg aaaaagtgga aagtccatgg ttacggatca cttaacctag
      721 acccgcttaa actgcaagat ggcctcaaca agggtgacga ttatctagcc aaaaacttga
      781 aagtcatgct caaaaagaat atcctaggcc acctgccatc aaatcaagtc gccgtcagtg
      841 taccgacatc caaaacctac agtcgaactc ttaacctacc cctggatgcc gaaaacaata
      901 tcaacgaagc cgttaggctc gaagccgacc agtacattcc ggtgccagtc gatgaactca
      961 atatcgatta tgagattatc gagcgcaaca aagagggcct gtcggttcta atctcagcta
     1021 cgcctaagaa gatcgtcgaa aatatcgtta ctagtttgga gacggctggc ctggaagtag
     1081 tgtttgctga gcctggagtc agttcaattg ctcggctact acgtcatacc gaagatggca
     1141 atctgccgac tatcatcgta gatattggcg ccgcctcgac cgacattgct atcttcaata
     1201 agactatccg ggtaagtggt ggcgtagcca taggcggtaa cagcttaact atcgctatct
     1261 ccaaaaagct agatataagc ctggaagagg ctcacaagat caaggtactc gatggtctgt
     1321 cgaccggacc caagcgcacc aaattggaag aagctatgga gccaagtctc aacaatattt
     1381 gtgacgagct aaagaagatg acgcgttact acaccgaccg actggagatg aaagataaga
     1441 ttgagcaaat catcatgtca ggcgccggct ctaacgtacc cggcatggct gactatttca
     1501 ccgacaaact agagactgcg gtacgcgtag ccagtccttg gcaaaagctc aaattcggca
     1561 agttgcccca gcccagcaag caaaataaac cacgcttcat cagcgctaca ggcctagcct
     1621 tcgtaacacc ggaggatgtt tggaaatgat caacctcctg tcatccgagc acaagagaaa
     1681 cttacgcgcc gcccgcgtca atgtgacact aaaccgttat gttaacttga tcatactatc
     1741 cgccgtattg gctggtgtga tatttggcgc cggctacttc ctgaccatgc acgaacgaag
     1801 ccaagctgaa gccggcatag ctgacaataa gcagcagatt gccaaatacg acaaaattcg
     1861 ccgccaagcc gaacaatttg agacaaacct aagaagtgct cgcgtaatcc taggtggtca
     1921 agtaactttt tctcagctta tcgttgacat agctaagacc ctaccggaag gtgctgtctt
     1981 gcaatctctc catatcgaga ctgaggatat cggtggcaag cctatcaccc tgtcagccag
     2041 aacgatcgat gccggcatca ctccccttac tcttaaaagt gccctagaac aatcgccgtt
     2101 gtttagcgag gttagcatca taagtatcaa acgaaaccta gtgacaggcg aacaacgtgt
     2161 tgaaaccagc caatccgact atcccgtgtc agtaaatctg tctgtcagtc ttgataaaac
     2221 agccggtaaa ccaggagata aatcatgaag aaaggccaac tgaccgccgt tcagctgatg
     2281 tacggcttat atggattgat cgcagtcctg ttgattgcga ccggggtggg tttctactac
     2341 gccaagaact ttttgggttc agctgtggcc gacactaacg acctcaaaga tcaagccagc
     2401 gtcagccagc aaaatgtagt caaactgcaa caactccaga acgaactcca cagccaagct
     2461 gatgtagtcg agcgaaccag acaaatcatg gccgacagcc agcattatca gtatcaagac
     2521 cagatagtca aagatatcaa cacctacgcc aaccgcaccg gcgtacaggt catcggttat
     2581 gacttcggcg acccgactaa gaaatccgcc aataaatcta gtctaccaac tgtcagtggt
     2641 actcggatta ttaccgccga tttaaccctc gatgccccag tcaaatacac ccgtttctta
     2701 aactttctcc aggccatcga acaaaacctg accaagatgc aggtcaccgg tgtcaacata
     2761 acacccgatg aacaagacgc tgactatgta tctaaccccg gcatcagcct gcaaatatat
     2821 gtaaaaaatt gattatgagt aacaaaacta caaccactag ttccaaacaa ctgacagccc
     2881 agctaaaagc tgctggacaa ccgatagcca aacaccacgt cgtcatcgta gtagtcctat
     2941 ttcttggctt cttgacctac gccgtcttta cggtcagtca gatcctactg agtaacgacc
     3001 agtccatgga taccaaagcc caagagcaag ctatcactac caatttcgat caaaagacga
     3061 tcgagcaaat ccagcaactg aagagtcgac aacaatcgac cgacctcagc ctacccgaag
     3121 gacacatcaa tccattcgac tagctctcgc ttcgcttaag ctatgactac gtcgaagcat
     3181 tgctcactcg gcagtgtaag caagcttccg ctgctctcgt ctcgcttgag ctatacctct
     3241 ggttagcacc agctccctca ggaacgagaa caagctctca ttcctctcgc ttcgcttaag
     3301 ctataataaa ggtatgcttt tgcctatcga acggtttcta ggtgcaccgc tacttagcct
     3361 acaactagaa gctaagatcg ccagtttagc cgaaccgatc attgatccta ggcaacttaa
     3421 gcttgtggcc ttctacgtcg acagccctaa atcggtcgat gaggacttag tcatctttag
     3481 tagcgacatc cgcgaagtaa accagcagct cggcatgttg atcgataacg acgacgttat
     3541 cacaccactc gacgacttag tccgactaaa cgagattatc gaatttaact tcagcctacc
     3601 gggtgttaaa gttgttgacg agcgaggccg caaatacggc aaggttacca gctacaacct
     3661 cgaccccaat agcttctttg tcgaacagtt agtagtcgaa ccaagtattt ggaataactt
     3721 aaacaatgcc aacaacttgg ttcaccgcaa tcagatagta tcggtcacca ataaacgtat
     3781 tgtcatcaag gcaccgacag ttaaagcaaa gcgagaaact cgtcagacga ccgatttcgt
     3841 caacccgttc aaagaactta aacagcctga aagtagccgc ccggcgtaag ccacggcccc
     3901 tacacgctac ctaccaaccc ggcgcttgcc cgccattcct ccaaagagac acagaaattt
     3961 aaagattcta aatttcttat ggcggaaagc caagcctctt tgtataaaag ccggacatcg
     4021 ccactagcga ccactcggac ggtatctctt cagcgaaggc tctttggcat agccaactgc
     4081 cgagaggaag acggtaccgg cccaagtcgg tgcgagacag cgaagctacg tgacagcgta
     4141 acgctgctga cgacgcggag cggtgagagg taagcagcgg caggtgatcg tcgggttgag
     4201 tcagtcgctt aaggcattct ttatatcgtc gtaggaaaag ccttgtcgag ccagataagc
     4261 gataaacttt tgctcatccg gatagcgtga gcgtttcttg gcgataatct ttttgatctc
     4321 atcattgtca ctacgttcag tcacttccag ctgttgatcg attatggctc ggtcgacacc
     4381 cttactcctc agttcagcta ctaatttacg ccttgacgca cctttgctta aactacgatt
     4441 ctccaaccag tagcgagtaa agctctcgtc atttaagtac cctttatcaa gcagtcgctc
     4501 caataccctg tcggcaatag actgactgac gccgggtcgt tctttcagct cgccggtctt
     4561 tttcgactta tagcgcttag ctagtgtctt gcgccttaga taatcacgaa cttctttgac
     4621 ggaatgcggc cgcatcatac agtactccag agctctagtg tagagtttgc caaattggct
     4681 ctcttcctcg agttggacca gctcttcttc gccaatatcc cgaccgactc tgatgtctag
     4741 ttcggtaacc tgtaggacgt cgagagagaa ccgatatttg ccatcaagtg atacattaac
     4801 ccggttttta tcctgaatct gtgcagtaat ggcggtaatc ttcaccgctt ctaacctttt
     4861 taaacgtcgg cttcagcgac cttagcacga actgctttgt cgatctcagc tagcaccttc
     4921 gggttctcgc gcaagtattc tttagctgct tcacggcctt ggccgatctt gccgtcctta
     4981 taatcaaacc aggcaccaga cttgccgact ataccgtgaa ctactgctaa gtccagtacg
     5041 tcacctgtct tggaaatacc ctggttatac atgatgtcga actcggctac cctgaacggc
     5101 ggggcgatct tgttcttaac gaccttaact ttagctcggt tgccgatgac atcttcaccg
     5161 actttgatct gaccgatgcg gcggatatct aaacgaacgc tggagtagaa ttttaaggca
     5221 ttaccaccgg tggtagtttc tggattacca aacatgacac cgatcttcat acggatctga
     5281 ttgataaata taacagttgt cttggattta ttgatggcac cagttagctt acgtagggcc
     5341 tggctcatca gccgggcttg taggcccatg tgtgagtcac ccatatcacc ttcgatttca
     5401 gcttgtggca ccagagcggc gactgagtcg accacgacta ggtctacggc gttagaccgg
     5461 accaaagttt cagcaatttc cagtgcctgc tcaccgttgt ctggctggct aacaagtaag
     5521 ttgtcggtgt cgacacctaa tcgcttggcg taagccggat caagtgcgtg ctcggcatcg
     5581 ataaaggctg ccgtaccacc attcttctga atctcagcaa tcgcgtgcaa ggtcagtgtc
     5641 gtcttacccg acgattccgg cccatagatt tcaataatcc gtccctttgg atagccgcca
     5701 ccaagggcca aatccaggct cagcgcaccg gacggagtag ttgctacatt tactttaccg
     5761 gcgtcgccca gtttcatgat cgatccatca ccataagctt tggtgatgtg ttccagtgcc
     5821 agtcctaggg cttgagattt tccgtcttta gccgcatcgt ttttagcagc tgttttggtc
     5881 ttcttatcag ccatttactc ctccatcttc tttactctta ttataccctg ttttaaaatt
     5941 aagttaatga gaaaagcaca aggctttacg gtcgtagagg ttatcctcgt tatcgtcgta
     6001 ctggtagcgg caggtttgat tttttggcac gaaaaatcag tagtcgatgc caagaaccgt
     6061 gacttaacac gcaagaccgc tatcaatgcc atagcttatt cacttgataa tgtttaccac
     6121 gttaagcacg gctactatcc ggatagcttg aacgataaga cattcatggt aatcgatccc
     6181 gccatcatgc gagacccgcg tggccatgca gtaggtgata aggattctga ctatcgctat
     6241 gaacccacta actgcgccgg tggtcagtgt cagggatata gcttaagagc gcaactggaa
     6301 tacgaagccg actacgtcaa gtctagcgat gatcacgcct ctgactaatg gttgccgacg
     6361 ggacgaccat tcttgtaagc ttcaactgca tcattgagta acttgacctg gagggccggg
     6421 tgacctacat aggttagtcg gtaggtattt tcatcaccga cggtcgatag acgaatcttg
     6481 ccgtagttaa aaatgtactg gacgatgccc gacttaaaaa agctaacatc ttcgacacta
     6541 ccgaggctaa ctgactgagt acgctgggca aataagctaa ggcgaacttg ctgaacgata
     6601 ccctcgttgg tcaagaaaag ttggttgctg ttatagacat aggcactgat gtaaccacca
     6661 atcaggaaca gaagacctaa agcaaatacc gcaccaatac caagcagtcc agcttccctc
     6721 atgatgccgt tattccacag tattgctggc actagaacac aaacggccag tataaggata
     6781 gtcaccgtag cccaaatcga caataaaccg atgatatgac gcttgattac ggtgatgacg
     6841 tattcacctt ccgataaatt aagttgaggg taggcttttt tggacgcttc gtgcttcttg
     6901 gctatctctg ctgagacttc cggcttgggt ggctccagcg gacggctggc gtgaacgact
     6961 tgtggctgtg ccggtgcggt ctcggcggga tgtaagtaga gcggtcgacc ttcggtgtcg
     7021 tag
//
LOCUS       DASSBW010000008         8424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1759489, whole genome shotgun sequence.
ACCESSION   DASSBW010000008 DASSBW010000000
VERSION     DASSBW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759489"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFG56_00175"
     CDS             <1..510
                     /locus_tag="VFG56_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET6622336.1"
                     /translation="RYTQLKRSWNGNLALNAYLRIFLVQAVLAWVIALPIVIVNQAEP
                     RPLDWLALVGGLVWLIGFALEAIGDQQLRTFVSDPKHRGKLMDSGLWRYSRHPNYFGE
                     LTQWWGIFIIALSTPMGWIGFIGPLTLTILILFISGIPLNEKRQTAKLGWQEYKRRTS
                     SLVPLPPKK"
     gene            554..904
                     /locus_tag="VFG56_00180"
     CDS             554..904
                     /locus_tag="VFG56_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622337.1"
                     /translation="MQKRIYDREIKRQHRQEDRSRAYADKLMADVPDDYPALDHLREM
                     YQLDYYLENGYDCSEQELLDAIEACKIEAIQALWDFTDTVEVTGERPSTANALGRTAM
                     SNSNGLLEPDAIGA"
     gene            1051..1281
                     /locus_tag="VFG56_00185"
     CDS             1051..1281
                     /locus_tag="VFG56_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622338.1"
                     /translation="MSESLGQSPENAEEFSRERALSGLIHEYFPEDEEFFGEMDFEDR
                     LGAVYGQLLESGEDPDLVLGEAGVLEDDENEV"
     gene            1271..4150
                     /gene="ileS"
                     /locus_tag="VFG56_00190"
     CDS             1271..4150
                     /gene="ileS"
                     /locus_tag="VFG56_00190"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET6622339.1"
                     /translation="MKFKSGTRRRAIEYEKDWVKRWKENQTFEKSVEQRPKDDSYVFY
                     DGPPFISGVPHHGTLLSSIVKDAVPRYQTMKGKRVERLWGWDAHGLPAERYTEKKLNI
                     HSREEVLEYGLEKYIKATRANMLETSSAWEDVIDRIGRWVEFKGAYKTMDKDYMESIW
                     WAFKTLYEKGKIFEGEKVLMYCTLDGTPLSKAEVTMDAGAYQDVTDPSVYVKFKLDDD
                     STLLAWTTTPWTLPANTAVAVNKDFDYVEVKVGDEQFILAKDLTDKVLTDEKHQPLEY
                     KVVRELKGKELVGKSYQPLLGLDRGKNAHKVWHAPYVSDEDGTGIVHLAPAYGEEDFE
                     LAKERKVPVVHVIDQNGYYTEGDWRGENVWEANKPIAKALHERGIVWKIDYIRHSYPH
                     CHRCGTRLMYRAHPSWFMDIDGQRTKMLEQNGDINWFPPHVKNGRFAKTIEQAPDWNI
                     SRDRFWATAMPVWKSESGKTKVIGSYAELKELSGKELDDYHRPWIDDVKFKIDGEEYT
                     RIDKVMDSWFEAGSMPFAQFHYPFENKDKFEQNFPGDFIVEYIGQVRAWFYYMHVMAV
                     ALFGENSFKNVIVTGNVAGNDGRKMSKSYGNYTDPNELMDKFSADSLRFLLLSSPLLN
                     GEDFALQDKEVGDVARKLSMIWNMYDFFTMYAEVDGWEFDGQLKDPLTDLTNPLDVWI
                     VSRLHQLAAEVEEHMDDYNIPDALAPILPFLDDASNWYVRRSRRRFWKSGKTADKNDA
                     YKTLHYVLVRLSYVLAPFTPFLAEELYHKLTGDEESIHLKDWLAAGNVNELVMNDMER
                     TRFIVNHALSGRARLNIKTRQPLASIEIRTSEKIDNEAKSIIQDEVNVKNVNVVFDKS
                     KTGYTMEIGLDGKLTPELKREGLMREIVRHVQSARKAAGLNVDDRISLVLSSDAKEVH
                     QAVKEHTDAIKAETLAVDLSDKGQRAFETSVKADGYQVDIALEKS"
     gene            4276..4575
                     /locus_tag="VFG56_00195"
     CDS             4276..4575
                     /locus_tag="VFG56_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622340.1"
                     /translation="MSVTTEETTLGRELSEYLGELECLAQQGAQSVSALDVMSRLTNI
                     CIDTQTAVWKLPERTQEILQQARQVSQVTDHHGLCGNETLLLRLVTRHLRESLAV"
     gene            complement(4641..5444)
                     /locus_tag="VFG56_00200"
     CDS             complement(4641..5444)
                     /locus_tag="VFG56_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6622341.1"
                     /translation="MKMFKTVLSKSLYNLRWAAFGWSLVVFFIAFLTMIVFDAINQQG
                     IEAIVGQTPDSLKSLVGTASDFTTISGYIGQQIFGPNGIMFTIIMSIILFISISASKE
                     DNGQLQTLLTFPISRSRVYFEQWLAVALVIGLVCLWFFPAVWLALAILGHSTDWTRLW
                     LSIFECFLMNLSYGLVAYGTAMAFGKKGLTILIGAGYAAISFIVSSLSPAVDWLAWPD
                     KLSIYHYYNQPQVMSAGLDGSDVGVLFTIIAGLTLVGWLGFTRRDIRAS"
     gene            complement(5431..6336)
                     /locus_tag="VFG56_00205"
     CDS             complement(5431..6336)
                     /locus_tag="VFG56_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6622342.1"
                     /translation="MTKSTLALKLDHVSKTYGRTRGIKDVSLEVKNGEIFGFLGPNGA
                     GKSTTINTILDLLKPEQGRIELFGLDHHKQLKKTHQGIGYLSGDMETDPTLTGRQYLS
                     FAANLRGGVDPERIKQLSTRLKCDLDRKIRHLSRGNRQKIGLVAALMHEPDLLILDEP
                     TSGLDPLIQNEFNQIIKEHQAKGKTTFMSSHVLSEVQEICDRVGFIKDGRLIKVARMD
                     KLMAEAARQVSVVFESTAPDSRLKTLPGVSNYRSSGQRRNFDFNGDYNLLLRVLSYKT
                     ITDIEIAAPDLEELFMSYYQGDEDV"
     gene            6391..6771
                     /locus_tag="VFG56_00210"
     CDS             6391..6771
                     /locus_tag="VFG56_00210"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET6622343.1"
                     /translation="MTIDDQRFEAMISKALDELPEEYVKNLDNVAITYADQPTPEQRI
                     KMKLKGGESLFGLYEGIPLTKRGAGYTFVLPDKITIFKHPMAEMTQTDDQLYGQVKHT
                     LWHEVAHFYGLDHDRIHEIEARWR"
     gene            6860..7297
                     /locus_tag="VFG56_00215"
     CDS             6860..7297
                     /locus_tag="VFG56_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622344.1"
                     /translation="MTEQAPRQRLYEGPPKKINDVPRREEEIRSRSGMPGGRSYSDHL
                     TDQYGQEGRGWEFATDQYDAENKMDSVEAETQHALAREALGNRALTGHQETDDGVRLS
                     NRQIEEYTRTAHPPKGDILVYPDGDVHQADKLNDAASEEAKGE"
     gene            7302..7550
                     /locus_tag="VFG56_00220"
     CDS             7302..7550
                     /locus_tag="VFG56_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622345.1"
                     /translation="MAEAAKHVPVEFKESRREQIAVDHEHFETVELPEQSDVEKAEGR
                     RELAELKLRFGGSVLDQVGNQLLVGNEDYDEFNPHEAA"
     gene            7537..7818
                     /locus_tag="VFG56_00225"
     CDS             7537..7818
                     /locus_tag="VFG56_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622346.1"
                     /translation="MRRPKKELYMLVLKHKHHLDFPHLTFNLPRAMSLSPVMADDDTL
                     VKSIDRDADELDNNWTLNERPDSSDLERFWQRVTSEIKTDPDWVDFTVD"
     gene            7890..8219
                     /gene="rplU"
                     /locus_tag="VFG56_00230"
     CDS             7890..8219
                     /gene="rplU"
                     /locus_tag="VFG56_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET6622347.1"
                     /translation="MKAIVRIGGQQFMVAEKDTLLVDRLPEGTKELTLEPLMIVDGKD
                     SKVGAPVVAGVKIKAKVLDEEVKGDKVLSIRYKSKKRVHKVRGHRQKYSRIEVGKIGA
                     TKPAAKK"
BASE COUNT         2340 a         1953 c         2231 g         1900 t
ORIGIN      
        1 cgctacactc agctcaagcg tagctggaac ggtaacctag ccctcaatgc atatttgaga
       61 attttcttag tccaggcagt tttggcgtgg gtgatcgctt tgccgatagt gatagtaaac
      121 caggctgagc cccggccgct ggattggcta gccttggttg gcggtctggt ctggttaatc
      181 ggctttgccc tggaagctat tggcgaccag cagctcagaa cgtttgttag cgacccgaag
      241 caccgcggta agttgatgga cagtggcctg tggcgttatt cgagacatcc caattacttc
      301 ggcgaattaa ctcagtggtg gggtatcttc atcattgcct tgtcgacgcc catgggatgg
      361 attggtttca tcggtccgct aactctgact atcttgatat tgttcatctc cggcatacct
      421 ttaaacgaaa aaaggcagac cgcaaaacta ggttggcaag agtataagcg aagaactagc
      481 agcttagtac ctctgccgcc taaaaaatag gcttgtgatt gaccgatata gcgcgtattt
      541 taagatagtg agtatgcaaa agaggatcta cgaccgcgaa ataaagcggc agcatcgtca
      601 agaagaccgg tcgcgcgctt acgccgacaa gctgatggca gatgtgcccg atgactaccc
      661 ggcactagat cacttaaggg aaatgtacca actcgattac tacctggaaa acggctatga
      721 ctgcagtgag caagagttgc ttgatgcaat cgaagcttgt aaaatcgaag ctatacaggc
      781 actctgggat ttcaccgata cggtagaggt aacaggtgag cggccatcaa ccgcgaacgc
      841 tttgggaaga acggccatgt ccaactcaaa cggcttatta gaacccgacg caatcggcgc
      901 gtaagaccga acaaactaag cgctaagaag aacggcgccc ctaatctgtt ttacggggta
      961 taagagtctc tttttagaaa ctggatggta ccgtccgcaa aagcggattc cagtgactaa
     1021 aaagggattt ttgtaattta aggagtaaag atgagcgaga gtctaggtca aagtccggaa
     1081 aatgctgaag aatttagtcg cgagcgagcg ctaagcggac ttattcatga atactttccc
     1141 gaagacgaag agtttttcgg cgagatggat ttcgaagacc gactcggtgc ggtctatggc
     1201 caactacttg agagcggaga agatccggat ttggttctag gtgaagctgg ggtattggag
     1261 gacgatgaaa atgaagttta aatcaggcac tcgccgacgg gcgattgagt atgaaaaaga
     1321 ttgggtcaaa cgttggaaag aaaatcagac gtttgaaaag tcagttgagc aacgaccaaa
     1381 agacgattct tatgtctttt acgatggacc accgtttatt tctggcgtgc cacaccatgg
     1441 cacactattg agttccatcg ttaaagatgc cgtgccgcgc tatcaaacca tgaaaggcaa
     1501 gcgtgtcgaa cgactgtggg gctgggacgc ccatggacta ccggccgagc gttacaccga
     1561 gaaaaaactt aacatccact cccgcgaaga agtgctggaa tatggcctgg aaaagtacat
     1621 caaagccact cgcgccaata tgctggaaac tagcagcgcc tgggaagatg tgattgatcg
     1681 cataggccgt tgggtcgagt tcaagggtgc ctacaagacc atggacaaag actacatgga
     1741 aagcatatgg tgggcattca aaaccctgta cgaaaaaggc aagatattcg aaggggaaaa
     1801 ggtactgatg tactgtacgc tcgacggtac gccgctttca aaagccgaag tcaccatgga
     1861 tgccggcgcc tatcaggacg tgaccgatcc gagcgtctat gtgaagttta aacttgacga
     1921 cgactcgacg ctgttggcgt ggacgacgac accctggacg ctgccagcca ataccgccgt
     1981 agccgttaac aaggactttg attacgtcga ggttaaagtt ggcgacgaac aattcatctt
     2041 agcgaaagat ctgaccgaca aggtcctgac tgatgaaaaa catcagccac ttgaatataa
     2101 agttgttcgt gagcttaaag gtaaggagct ggtcggtaaa tcctatcagc cattacttgg
     2161 tctcgaccga ggtaagaacg ctcataaggt ttggcatgct ccatatgtta gcgacgagga
     2221 cggtactggt atcgtccatt tggcaccggc ttacggtgaa gaagacttcg aactggctaa
     2281 agaacgcaag gtaccggtcg ttcatgtcat cgatcagaat ggttactaca ccgaaggtga
     2341 ctggagaggc gagaacgttt gggaggccaa taagccgatc gccaaggcgc tgcacgaacg
     2401 tggtattgtc tggaaaattg actacatccg gcacagctat ccgcactgtc accgctgcgg
     2461 cacgcggctg atgtatcggg cacacccgag ctggtttatg gacatagatg gccagcgcac
     2521 caagatgctc gagcagaatg gtgatatcaa ctggtttccg ccacatgtca aaaacggccg
     2581 ttttgccaag actatcgagc aggcaccaga ctggaacatt agccgtgacc gcttctgggc
     2641 aacggctatg ccggtttgga agtcggagtc cggcaaaacc aaggtcatcg gcagctatgc
     2701 cgaattgaaa gagttgagcg gcaaggagct cgacgactac caccgtccat ggatcgatga
     2761 tgtgaaattc aagattgatg gtgaagagta tacccgtatc gacaaagtca tggatagttg
     2821 gtttgaggct ggctcgatgc cgttcgccca gttccactat ccgtttgaga acaaagataa
     2881 gttcgagcag aatttcccgg gcgatttcat cgtcgagtat atcggccaag tccgcgcctg
     2941 gttttactat atgcacgtta tggcagtagc attgtttggc gaaaattcgt tcaagaatgt
     3001 catcgtcacc ggcaacgtcg ccggcaacga cggccgcaag atgagtaaaa gctacggcaa
     3061 ctacaccgac cccaacgaac tgatggacaa attctcagcc gattcactcc gcttcctgct
     3121 gctgtccagc ccgctactaa acggcgaaga tttcgcgttg caagacaaag aagttggtga
     3181 cgtggcccgt aagctttcga tgatctggaa tatgtacgac tttttcacaa tgtacgctga
     3241 agtcgatggt tgggagtttg acggccagct aaaagatccg cttacagatc tgaccaatcc
     3301 actcgatgtc tggattgtgt cgcgcttgca ccaactagcg gctgaagttg aagaacatat
     3361 ggacgattac aatatccctg acgctctagc gccgatacta ccgtttctcg atgacgcttc
     3421 taattggtac gtacgccgtt cccgacgccg tttctggaag tcgggtaaaa cggcagacaa
     3481 aaatgacgct tataagacgc tgcactacgt cttagtacga cttagctacg tactagcgcc
     3541 attcacgccc ttcttggccg aggagctgta tcacaaactg acgggcgatg aagaatcaat
     3601 ccacttgaag gattggctgg cggccggcaa tgtcaatgag ctggtcatga atgacatgga
     3661 gagaactcgt tttatcgtaa atcatgcact ttcgggtcgc gctagattaa acatcaaaac
     3721 ccgacagcca ctcgccagta tagaaatcag aacgtcagaa aaaattgata atgaagctaa
     3781 gtcaatcatt caagacgagg taaatgttaa gaatgttaat gttgtgtttg ataaatccaa
     3841 aaccggctat acgatggaaa tcggtctaga tggtaagctc actcccgaac tcaagcgtga
     3901 aggcctgatg cgcgagatcg tccggcatgt tcaatcggcg cgtaaggcgg ccggcttgaa
     3961 cgtcgacgac cggattagct tggtgctcag ctccgacgcc aaagaagtcc atcaggcagt
     4021 caaagaacac accgatgcca tcaaggctga gaccctggcc gtagacttga gcgacaaggg
     4081 tcagagggcc tttgagacat ccgtcaaagc cgatggctat caggtcgata tcgctctgga
     4141 gaagagttaa gtcttgccca gctattgaca ggcgtcagtt aagctcgtat aaccaaagta
     4201 ttatctagga gacaaaaagt ttttaggtaa gtagtttgta aggcaatctt tcagtcaatc
     4261 ttggaggtga cgacaatgag tgtcacgact gaagagacca ctcttggccg agagctgtcc
     4321 gagtatttgg gcgagctcga gtgtctagct caacagggtg cacagagtgt gtcggctctc
     4381 gatgtgatga gccggctgac caacatctgt atcgacaccc agacggcggt ctggaaattg
     4441 ccagagcgga cacaggagat tctgcagcag gcgcggcagg tcagccaggt gacagatcac
     4501 cacggtcttt gtggcaatga gacgttgttg ctcagactgg taaccaggca cctacgcgag
     4561 agtctggccg tctagcggcc cttagtaagg ggtggtgtta aaaaatagca ccacccctta
     4621 aaaatgctta caactcttat ctaactggcg cgtatatcac gccgagtgaa gcctagccag
     4681 ccgacgaggg ttagaccggc tatgatggtg aatagtacac ctacgtcgga tccgtccaag
     4741 ccagcactca tgacttgtgg ctggttatag tagtggtaga tcgacaactt atcaggccag
     4801 gccaaccaat cgacggctgg gctcaaactg gagacgatga agcttatcgc ggcgtagccg
     4861 gcgccgatca gtatagtcag gccttttttg ccgaaggcca tggcggtgcc gtatgccact
     4921 aagccgtagc ttagattcat taggaaacat tcaaagatcg ataaccatag ccgtgtccaa
     4981 tcggtcgaat gaccaaggat agctagcgct agccaaactg ccggaaagaa ccatagacag
     5041 actaagccga taaccagagc cacggctagc cactgttcaa agtagactcg actacgactg
     5101 attggaaaag tcagcaaagt ctgcagttgt ccattatctt cttttgaagc actgatagaa
     5161 atgaagagga ttatcgacat gatgatagta aacatgatgc cgttgggccc aaatatctgc
     5221 tgaccgatat agcccgatat agtagtaaag tctgaggccg taccgaccag gctcttgaga
     5281 gaatctggtg tctggccgac gatagcctcg ataccttgct ggttgatagc gtcgaacaca
     5341 atcatagtaa ggaaggcgat aaagaagacg accaaagacc agccgaacgc cgcccagcgc
     5401 aagttgtaga gcgatttgct aagtacggtc ttaaacatct tcatctcctt ggtagtaact
     5461 cataaacaat tcttccaagt cgggcgcagc gatctcaata tcggtgatag ttttgtaact
     5521 cagcactctt agcagtaagt tatagtcgcc gttgaagtca aaattacggc gctgaccact
     5581 tgatcgatag ttgctcactc cggggagagt tttgagccta gaatcaggtg cggttgattc
     5641 gaagacgaca ctgacctggc gggcagcttc ggccatcaat ttatccatgc gagccacctt
     5701 gatgagtcgg ccgtccttga tgaaaccgac ccggtcgcaa atttcctgga cttcagataa
     5761 gacatgcgag ctcatgaagg tcgttttacc tttggcctgg tgttctttaa tgatctgatt
     5821 aaattcgttt tggatgagcg ggtcaagacc tgaggtcggt tcgtctagta ttagtaggtc
     5881 tggttcgtgc atgagagcgg cgaccaggcc tatcttttgg cggttaccac gcgataggtg
     5941 acggatctta cgatccaaat cacacttcag ccgagtcgat agctgtttga ttcgttccgg
     6001 gtcgacgccg ccgcgcaaat tggcagcaaa gctcaaatac tgtcgaccgg ttagagtcgg
     6061 atcggtctcc atatcgccag ataagtagcc gatgccctgg tgggttttct taagctgctt
     6121 gtggtgatcg agaccaaaca attcgatccg gccctgctcg ggcttcagta gatcgaggat
     6181 cgtattgata gtggtcgact taccggcacc attgggtccg aggaaaccga agatttcacc
     6241 gttttttact tccagggaaa cgtcctttat gccacgggtt ctaccatagg tcttgctaac
     6301 gtggtcaagt tttagggcta gggtggattt agtcatttgt aaatagctta tgttctgcgg
     6361 ttaatgactg tcaaatgcta gactagcaat atgacaatcg atgaccagcg gtttgaagcc
     6421 atgatatcta aggctctaga tgagctgccg gaagaatacg tcaaaaatct cgacaacgtg
     6481 gctataactt atgccgatca gccgacacct gagcagcgga taaagatgaa gttgaaaggt
     6541 ggcgagagcc tatttggttt atacgaaggg atacctttaa ccaagcgcgg ggcgggctat
     6601 acgtttgtac tgccggataa gattactata tttaaacacc cgatggccga gatgacacag
     6661 accgatgatc agttatacgg ccaggtcaag cataccttgt ggcacgaggt agctcacttc
     6721 tacggtctag atcatgaccg catccatgag atcgaagcgc gctggcggta aatcagtagg
     6781 agaggttagt tgacaattta tactgtttat gctagaatgt gtaacagtaa actaaacgaa
     6841 acaaaaacga gcccaccaca tgacagagca agcaccgcgt cagcgtctct acgaaggtcc
     6901 acccaagaaa ataaatgatg taccgcgccg cgaagaagaa atccgcagcc gcagtggcat
     6961 gcccggcggt agatcatact ccgaccactt aactgatcaa tacggtcaag agggtcgagg
     7021 ctgggaattt gctactgatc aatacgatgc cgaaaacaag atggacagcg tcgaagccga
     7081 gacccaacac gcattagctc gtgaagcgct tggcaacaga gctttgacgg gtcatcagga
     7141 aaccgatgat ggcgttcggc tatcgaaccg acagattgaa gaatacaccc gaacagccca
     7201 tccgccgaag ggcgatattc tggtctatcc ggacggagac gttcatcagg ccgataagct
     7261 taacgacgct gcgtcggaag aggcaaaggg ggagtagtaa tatggcggaa gcagcaaaac
     7321 acgtgccggt tgaatttaaa gaatcccgac gcgaacagat tgccgttgat catgagcatt
     7381 tcgagacggt tgaactaccg gaacaatcag atgtagaaaa ggccgagggg cgcagggaac
     7441 tggctgaact taagctaagg tttggcggct cggtcttaga ccaagttggc aatcaactgt
     7501 tggtcggaaa cgaagattac gatgaattta acccgcatga ggcggcctaa aaaggaactg
     7561 tatatgctag tactaaaaca caaacaccac ctagactttc cgcacttgac ctttaactta
     7621 ccgcgggcga tgagcctcag tccggtcatg gccgacgatg acacgttagt taagagtatc
     7681 gaccgagatg cagatgaact cgacaacaat tggacactca acgagcgacc ggatagcagt
     7741 gacctggaga ggttctggca gcgggtaacc agtgaaatta aaactgatcc cgattgggtc
     7801 gatttcacgg ttgactagag tgactttaca agctcatttg tctctgttac aatcaatcct
     7861 cgagtaattt aagtttaagg aaagtacaag tgaaagcaat cgttcgtatc ggcggccagc
     7921 aattcatggt agccgaaaaa gacaccctcc tggtggaccg cctccctgag ggaaccaaag
     7981 aactgacctt agagccgcta atgatcgtcg acggcaaaga ttccaaggtt ggcgctccgg
     8041 tcgtagccgg cgttaagatc aaagccaaag tcctcgacga ggaagtcaaa ggcgacaaag
     8101 ttctatctat tcgctacaag tccaagaaac gcgtccacaa agtacgtggt catcggcaga
     8161 aatacagtcg gattgaggtt ggcaagattg gcgcgacaaa accagccgcc aagaagtaag
     8221 cggtccgata aatataagac cacccgaacc acagccgggt ggtttttgtt ttgaaagctc
     8281 ttaagcttgg gccgcaaagt ggtataattg ccttagataa gatgaggcaa ataaagagtt
     8341 gagtaaaccc acgatcatcg cggtgaccaa tcaaaagggc ggcgtcggta agacgacgac
     8401 cagtatcaat ctgtcatact gcct
//
LOCUS       DASSBW010000009         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_980566, whole genome shotgun sequence.
ACCESSION   DASSBW010000009 DASSBW010000000
VERSION     DASSBW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980566"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1187
                     /locus_tag="VFG56_00235"
     CDS             102..1187
                     /locus_tag="VFG56_00235"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET6622348.1"
                     /translation="MLKKIKNLNWRILKPNRWWKWLAYVVVVFLALMYTARWWNPIIF
                     PPVKEPHFGVSFSIKQADIFDSDWKKNYLALLDDMGIKHYRLMSYWDMYEKTPGKYDF
                     SSLDWQIDQASKRGADVTLAIGLRQPRWPECHQPNWATSLKQTNYLKWKQSLEKYISV
                     VVKRYEHNPAVISWQLENEALNNWFGECGPAQRQRIIEEYKLVKSISQKPVWMSLSDQ
                     HGLPLGQPWPDKFGFSVYRTVWNEKIGPVKSYMTYPTPIWYHNLRRAVIKFYTGRDSF
                     IHELQLEPWGPIGTVKLSVQEQNKSMDLDQIHDSLLFARELGMQDIYVWGGEWWYWRK
                     QNGDPSVWNKVKSEIEPYIRKEVSTKY"
     gene            complement(1191..2831)
                     /locus_tag="VFG56_00240"
     CDS             complement(1191..2831)
                     /locus_tag="VFG56_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6622349.1"
                     /translation="MIADINVTEKSFGPNTLMSGVKFSVGSKEKVGVIGRNGIGKTTL
                     FSILTGADKDFSGDIIYRKGISVVSTAQEHHVSDHQTVLQYILTGLPEYESLSHVIET
                     YPASMGDDMKKIAEYTEALQRFDDKGFYQIEEKVTRELDNFQLPDIAHRPFSSLSGGQ
                     KRLVEVVKVMHSEAQLALIDEPTNHMDYVAKAQFIDWLKSASEAMLIITHDRDVLREI
                     DRIVELKDGAAHSYKGNYDDYLRQNAHQTGSAMNEYEVVKRRIDNLKIKVVQFRRLKE
                     RARDPDTIKQFKRRESQAAAELFELQQIDKPTFWIDKDSVEALNYKVASQYDKFKAQN
                     IKMKLKSDQSRSSHVLVKAVSLSLGYDKPLFGGVNIDLREGEAAELRGRNGAGKTTLI
                     KALLGRGQDIKNFAGELTLDPQVRVGVYEQEVSPKYFDLGLHDAIERIYLDLGLSVGE
                     TKIRQLMSDYLFTDGDSKTPVARLSGGQKARLQIIQMLANDPQLLILDEPTNHLDLPS
                     IEELESALSKYSGAVLYVSHDGYFRQAISGQTIEISRK"
     gene            complement(2875..>3871)
                     /locus_tag="VFG56_00245"
     CDS             complement(2875..>3871)
                     /locus_tag="VFG56_00245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6622350.1"
                     /translation="MKVSVVSESEFSVKGHGVHTAYVEHVNSLKALKNVTVQVNKFGS
                     ADITHIHTVGVYSLAHLLLGSSKKVISAHVVPASFVGSLIFAKWWLPLAKLYLRWFYN
                     RADLVLAVSDETKRELLRLGVKQPIEVMYNSIDTNVYKKTVRNRLAARTKLSLKDSDW
                     LVVGAGQVQPRKRIDSFVAAAKALPKMKFIWVGGMPFKRVAADYDKMNRLMKKTSSNT
                     NFTGVIELEAVKDYYHAADVFLLPSSQETFGLVVIEAAASGLPVVVRDIHDYDETFRG
                     YAAMFKNDEQLIEVLKKLKNDKEFYASQIEASVKLAFKYDSKAAALRLINLYKSL"
BASE COUNT          977 a          962 c          913 g         1019 t
ORIGIN      
        1 taggtgtcgg taaacaaacc aatacgcata tttattacag acagtataac aaacttggcg
       61 aaaccggcct ctagcattac acttgaaggt aagaaaaatt tatgcttaag aaaatcaaaa
      121 acctcaattg gcggatacta aagcccaatc gttggtggaa gtggcttgcc tacgttgtcg
      181 tcgtcttcct ggccttgatg tataccgcca gatggtggaa cccgataatt ttcccacccg
      241 tcaaagaacc tcactttggc gtcagctttt cgatcaagca ggccgacatc ttcgactcgg
      301 actggaagaa gaactacttg gcacttttag acgatatggg tatcaagcac taccgcctga
      361 tgagttactg ggatatgtac gaaaagacac cgggtaaata cgacttctcc agtctggatt
      421 ggcaaataga tcaagcgagc aaacgtggtg ccgacgttac gctggctatc ggcctgcgtc
      481 aaccacgctg gcccgagtgt catcaaccaa actgggcaac tagcctcaag caaaccaact
      541 atctgaaatg gaaacagtcg cttgaaaaat acatctcggt tgtcgtcaaa cgctacgaac
      601 ataatccggc cgttatcagt tggcagctgg aaaacgaagc tctcaacaac tggtttggtg
      661 aatgcggccc ggctcaacgc caacgaatca tcgaggagta caaactggtc aaatctattt
      721 ctcaaaaacc agtctggatg agcctatccg atcagcatgg cttgccgcta ggtcaaccat
      781 ggccagacaa attcggcttc tcagtctacc ggacggtttg gaacgaaaag atcggtccag
      841 tcaaaagtta tatgacttat ccgacaccga tctggtacca caatttgcgc cgcgccgtta
      901 taaaatttta caccggacgc gacagcttca tccacgaact tcagctcgaa ccctggggtc
      961 caatcggcac agtcaaattg tccgtccagg agcagaacaa gtcgatggat ttggaccaaa
     1021 ttcacgacag tttgttgttt gcccgcgaac tcggcatgca agatatttat gtctggggag
     1081 gtgaatggtg gtactggcgc aagcaaaacg gcgaccctag cgtctggaat aaagttaaat
     1141 ccgagatcga accctacatc cgaaaagaag tttcgactaa gtattagtcc ttacttgcgt
     1201 gatatttcta tcgtctggcc cgatatagct tgccggaagt atccgtcgtg actaacgtag
     1261 agaacggcac ccgaatattt tgacaaggct gattccaact cctcaatgga cggtaaatcc
     1321 aaatgattgg tcggctcatc caatatcagt agctgtgggt cgttagccag catctgaatt
     1381 atttgtagtc gggctttttg accgccggac aatctagcta ctggtgtctt ggagtcgccg
     1441 tcggtgaaca gatagtcact catcagctga cgaattttgg tctcaccgac ggacagtcca
     1501 aggtcaaggt agatccgctc gattgcgtcg tgtagaccta agtcgaagta tttaggcgac
     1561 acttcttgct catagacacc gacgcgtacc tgtggatcga gtgtcagttc gcccgcgaag
     1621 ttcttgatgt cttgaccacg ccccagcaat gccttgataa gcgtggtctt accggcgccg
     1681 tttcgaccgc gtaattcagc cgcttcgcct tcgcgcaggt cgatattgac gccgccgaac
     1741 aatggcttat cataaccaag gcttaaactg accgctttta cgagtacatg gctagagcgc
     1801 gattgatctg acttgagctt catcttgatg ttctgggctt tgaatttgtc gtactggctg
     1861 gctactttgt agttcagcgc ctcgaccgaa tctttgtcta tccaaaacgt cggcttgtcg
     1921 atctgctgga gttcgaaaag ctcggccgcg gcctggctct cgcgtcgctt aaattgtttg
     1981 atggtgtcag ggtcacgtgc ccgttcttta agtcggcgga attgaacgac cttgattttc
     2041 aagttatcga tacgccgttt gaccacttcg tattcgttca tggccgagcc ggtctggtga
     2101 gcattctgtc ggagataatc gtcataattt cctttgtagc tatgagctgc accgtctttt
     2161 agctctacta tccggtcgat ctcacgcaat acatcacggt catgagtaat gataagcata
     2221 gcctcactgg ccgactttaa ccagtcgata aattgagcct tggcgacgta gtccatgtgg
     2281 ttagtcggct cgtcgatcag cgccaattgc gcctccgaat gcatgacttt gactacttcg
     2341 accagtcgtt tttggccgcc tgacaaagag ctgaatggcc gatgggcgat atccggcagc
     2401 tgaaagttgt caagctctcg tgtaaccttt tcctcgatct gataaaatcc tttgtcatcg
     2461 aatcgctgca gagcttcggt gtattcggct atcttcttca tatcgtcacc catgcttgcc
     2521 ggatatgttt ctatgacatg acttaagctc tcatactcag gcagaccggt caggatgtac
     2581 tgaagcactg tctgatggtc actgacgtgg tgttcctgag cggtcgaaac gacacttata
     2641 cctttgcggt agataatgtc gccactgaaa tccttatcgg cgccggtcag gatactaaag
     2701 agagtagttt tgccgatacc gttacggcca atcacgccca ctttttcttt tgaccccacg
     2761 ctaaacttga cccccgacat caaagtattg gggccaaacg atttttcagt aacattaata
     2821 tcggcaatca tccttgagta ttatataaaa gaagcggccg ctaagcaggc cttcttacaa
     2881 acttttgtac aggttgatca agcgtagggc cgcggctttg gagtcgtact taaaagctag
     2941 tttaacgctg gcttcgatct gagacgcata aaactcctta tcatttttta acttcttaag
     3001 aacttcgatc aactgttcgt cgttcttaaa catggcagcg tagccacgaa aagtctcgtc
     3061 gtaatcgtga atgtcacgga cgacgacagg cagaccactg gcggcagctt cgatgaccac
     3121 taggccaaac gtctcttggc tactcggcag caaaaataca tctgccgcat gataataatc
     3181 tttgactgcc tctaactcga tcacaccggt gaagtttgtg ttagacgagg tcttcttcat
     3241 aagacggttc atcttgtcat agtcggcagc tacccgcttg aaaggcatgc cgccaaccca
     3301 aatgaatttc atcttcggta aagctttggc ggccgccacg aagctatcga ttcgtttcct
     3361 cggctggacc tgtccagcgc caactactag ccaatcggaa tctttaaggc taagtttagt
     3421 tcgtgccgcc agacgatttc ggaccgtctt cttatagaca ttggtgtcga ttgagttgta
     3481 catgacctca atcggctgtt taacgccgag tcttagaagt tctcgcttgg tttcatccga
     3541 tactgcaagc actagatcag ctctgttata aaaccacctg aggtagagct tggccaacgg
     3601 cagccaccat ttggcgaaga tcagcgagcc gacaaacgag gccggcacca catgagctga
     3661 aataactttt ttactcgaac ctagtagtaa atgggctagg gagtagacac cgacagtgtg
     3721 gatatgagtg atgtcggccg aaccgaattt gttgacctgg acggtaacgt tttttagagc
     3781 tttaagcgaa ttgacatgtt cgacgtaagc agtatggacg ccgtgacctt tgaccgaaaa
     3841 ttcgctctct gatacgacac tgactttcat t
//
LOCUS       DASSBW010000010         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_198334, whole genome shotgun sequence.
ACCESSION   DASSBW010000010 DASSBW010000000
VERSION     DASSBW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198334"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..2761
                     /locus_tag="VFG56_00250"
     CDS             41..2761
                     /locus_tag="VFG56_00250"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HET6622351.1"
                     /translation="MLGSSLSFIFGLLTQWFIWLPIAAVLAFLAWRNYRKADLVEDVE
                     HVLLTLEIPRTNDKKELAAEQLFASLHGILRDKHELALSGGLQEHISFEVAAVGKLIR
                     FYVWVPKHLQNFVEGQIYAQYPTVQIAAASEDYTKRQFEHQVIYTSELVLTDNEMLPI
                     KTFQSFEVDPLAGITATLGKLEDASEEIWVQVLARPIADNWHRRASRYAESIKTGQLL
                     SGDILSGVGGWMLGALAALWRPPEAGSGGSTTRELSERDKTRIAEVEKKSTKLGYQVK
                     IRIAYLGDSEVTAKQRMQAIVGTFKQFNSTNLNGFRMSKPSFRRDDIAAYMSRFFIDR
                     GFILNIEELASVFHLPHTNVETPNIVWASSKTAEPPSKLPVLTGDRAVDDEISAFGLT
                     NFRGINQQFGLWRSDRSRHIYIIGQTGAGKSGLLELFALSDVYHNQGYAIVDPHGDFA
                     VNNMQFIPANRIEDVVYFNPADTANPVGFNPMEVHDPGMKNNISSEIIGVLKRMFGMS
                     WGPRLEYILRYTILALLDYPNSTMLDITRMLTDKKFRKEVLSYCTDTVVLQFWNIEFA
                     SWNDKFQAEAVAPVLNKVGAFTANPIIRNIIGQPKSTFDIRKIMDEGKILIVNLSKGL
                     IGEDNASILGSFIVTKIQLAAMSRSDIPKIEDRRPFYLYVDEFQNFATDSFATILSEA
                     RKYGLNLTVANQYVSQMEEVVRDAVFGNVGTMISFRVSADDSPLLAKQFEPQFESGDL
                     LQMHNRHFIINMVIKGEKAPAFSATTLSIPSAPSDYTSYIIESSRAKHSRQREAVENE
                     IKDLLTPKDQTPPQSQSQAKKWPIDRSAQPAKPAHRPVQPNQVASDDNDSEATKPKRS
                     RSRKRSRSRSRKNSTSGAEQQRSTAPNTAETSDPAAPKLTGGDEHEIKLH"
BASE COUNT          930 a          871 c          784 g          869 t
ORIGIN      
        1 atttgtaaag cacgcttttt taagcgacaa tataggtagt atgttgggct ctagtctctc
       61 ctttatattt ggcctgctga cccagtggtt tatttggttg ccgatagccg ccgtcctggc
      121 cttcctggct tggcgcaatt accgtaaagc tgatctagtc gaagatgtag agcatgtgct
      181 actaacctta gaaatcccgc gtaccaacga caagaaggag ttggcggccg aacagttatt
      241 tgccagtctc catggtatct tgcgcgacaa gcatgaattg gcgctgtcag gtggcctaca
      301 agagcacatc agctttgaag ttgccgccgt cggcaaattg atccgcttct acgtgtgggt
      361 acccaagcac ttgcaaaact tcgtcgaagg ccagatctac gcccaatatc caaccgtaca
      421 aattgcagcc gccagtgaag actacaccaa gcgtcagttc gagcaccagg tgatctatac
      481 ttctgaacta gtcttgaccg ataacgaaat gctaccaatc aagacatttc aaagctttga
      541 ggtcgaccct ctagccggca ttacggctac tctgggtaag ctcgaagacg cctctgagga
      601 aatctgggtt caggtattgg cccgtccgat tgccgacaac tggcatcgtc gggcatcgcg
      661 ttacgccgaa tcaattaaaa ccggccagct acttagtggc gatatcttaa gcggggtagg
      721 tggttggatg ctaggagctt tggcagcact ctggcgacca ccggaagccg gcagtggcgg
      781 cagcactaca cgagaactaa gcgaacgcga taaaacccgt attgccgaag tcgaaaaaaa
      841 gtcaaccaag ctgggttatc aagtcaagat acgcatcgct tatcttggtg atagtgaagt
      901 tacagctaag caacgaatgc aagccattgt cggtactttc aagcagttca actcgaccaa
      961 tctaaacggt tttcgtatga gcaaaccaag cttcagacgt gacgacatcg ccgcctacat
     1021 gtcacgcttc ttcatcgata ggggatttat actaaatatc gaggagctgg cctcagtctt
     1081 tcacttacct cacaccaacg tagagacacc caatatcgtc tgggcctcgt caaaaaccgc
     1141 cgaaccaccg tctaaattac ctgttttgac aggcgacagg gcagtcgacg acgagatcag
     1201 tgcttttgga ctgacgaatt ttcggggcat caatcagcaa ttcggcttat ggcgcagtga
     1261 ccggtcacgt cacatctaca tcatcggtca gaccggtgcc ggtaagtcgg gactattgga
     1321 attatttgca ctaagcgatg tctaccataa tcaaggctac gccatcgtcg acccgcacgg
     1381 cgacttcgct gtcaacaata tgcagttcat accggctaac cgcatcgaag acgtagtcta
     1441 cttcaatccg gctgacaccg ccaacccagt tggttttaat ccgatggagg ttcatgaccc
     1501 tggcatgaag aacaatatct cttcggaaat cattggtgtc ctcaagcgaa tgttcggcat
     1561 gtcctggggg cctaggttgg aatacatcct gcgctacacc atcttggcgc tgcttgatta
     1621 tcccaactcg actatgctcg acatcacccg gatgctgacc gataagaaat ttaggaaaga
     1681 ggtgttgagc tactgtacag atacggtggt attacagttt tggaacatcg agttcgcttc
     1741 ctggaacgac aagttccaag ctgaagccgt cgctccggtt ttgaacaaag tcggcgcttt
     1801 cacggccaac ccgattatcc gcaacatcat agggcagcct aaaagcactt tcgatattcg
     1861 caaaattatg gacgaaggca agatactaat cgtcaaccta tcgaaaggtt tgataggtga
     1921 ggacaatgcc tctatcctag gtagcttcat cgtcaccaag attcagcttg ccgccatgag
     1981 ccgctccgac atccccaaga tcgaagatcg ccgacccttc tacctctacg ttgatgagtt
     2041 ccaaaacttc gccaccgatt ctttcgccac tattctttct gaagccagga aatacggcct
     2101 caacttaacc gtggccaatc aatatgtctc acagatggaa gaagtggtac gtgatgccgt
     2161 cttcggtaac gttggtacca tgataagttt tcgggtctca gccgatgatt caccgctgct
     2221 cgccaagcag ttcgaaccgc aatttgagtc gggcgatctc ctacagatgc ataaccgtca
     2281 cttcatcatc aacatggtca taaaaggcga gaaggccccg gcattttcag ctaccaccct
     2341 atccatcccg tctgcgccta gcgactacac tagctacatt atcgaatcat cgcgagccaa
     2401 gcactcacga cagcgtgagg ccgttgaaaa cgagattaag gatttactta cacctaagga
     2461 ccaaactccg ccccaaagcc agtctcaggc caagaaatgg ccaatcgatc ggtcggccca
     2521 gccagctaaa ccggcccata gacctgtcca gccaaaccaa gtcgcttcag acgacaacga
     2581 cagtgaagcc actaagccta agcggtcgcg ttcacggaag cgctcgcgct cacgtagccg
     2641 caaaaactca accagcgggg cagagcagca gcgctctact gcaccaaata cagccgaaac
     2701 tagtgatccg gctgccccca aactaaccgg tggtgacgag cacgaaatca aactgcatta
     2761 agcttttgaa accttgagcc atatcgattg aaatcatata gaacatatgt tctatatgat
     2821 gatttcaatt atcgtcattg taaaaatgtt aaagctgcca cggaaaagcc agaaggctca
     2881 gactttgaag cgtaagcagt aagagcagta ggctaaacaa tcaattaaaa agatttgagc
     2941 tagaaaaagc tgggattaga acgtaaagta ggtcgggttt gttatatatt gtttgtgtcg
     3001 cacaatatat cttttacgac ttttaaagat atatcaaaag atgattaagc atacgggctg
     3061 tgcgggggag tttcccgcta aataaggctt gcgcggttta atcaatcatc tttaaggtta
     3121 tcgtcttctt ttttagactg ttagcctcat ggctttagcg ccgcagccat ttatgtttta
     3181 cgagcttaat gatcgatgtt tgcttttttc cacaatcatt tgctttttat gtattttgtt
     3241 gcccgactct cctctcacct gccttttgac aaaatttgac tttcctcacc tactatattt
     3301 ttatatagtt tttagttgtt ttggtgtatt acgttctata gaacaaaaat agaacatagc
     3361 ccctctagag tagggcttat ttgtaataag cctgtcccgg agttcgaacg tcaatgtaat
     3421 cgggtgtctt atggtgcgac tcaaagtaat ggac
//
LOCUS       DASSBW010000011         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1425175, whole genome shotgun sequence.
ACCESSION   DASSBW010000011 DASSBW010000000
VERSION     DASSBW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425175"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_feature    103..226
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            279..812
                     /gene="rplJ"
                     /locus_tag="VFG56_00255"
     CDS             279..812
                     /gene="rplJ"
                     /locus_tag="VFG56_00255"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET6622352.1"
                     /translation="MAISRDKKTELVKELSDILASAKLTAAAKYAGLSVTDMQELRRT
                     ARESDVTIKVVKNRLLRVAMEQSAKFKSADKSLLEGQLLYAYSETDEVAPAQVLAKFA
                     KTQPELELVAGFDADGKVLDTAAIKALADLPSKDQLRGQVVGVIAAPLTQMLGALNGA
                     QRGFAQVLSQQAEKTSA"
     gene            936..1331
                     /gene="rplL"
                     /locus_tag="VFG56_00260"
     CDS             936..1331
                     /gene="rplL"
                     /locus_tag="VFG56_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET6622353.1"
                     /translation="MPELNAAQKKIVDELNKLSALELSQIVKHLEDEWGVSAAAPAAV
                     AVAGPAAGGEAAAAEDEKTEFTVTLKEAGAQKVAVIKAVKELTGLGLGEAKGLVDNAP
                     SAIKEKVSKDDAEAAKKALEEAGATVELS"
     gene            1559..2065
                     /locus_tag="VFG56_00265"
     CDS             1559..2065
                     /locus_tag="VFG56_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622354.1"
                     /translation="MPELPEKQVKRLRSLISEAETNLAAAKELLISVIGEEAVSHGAP
                     EDVAGKVIEGVFDGQSMVGPDGKSFPVPANYASKSKLIEGDILKLTITEDGSFIYKQI
                     GPVARHQVIGTLAQHDGAYYVEVGGKEYRVLLASVTYFKAKVGDQVSIVIPEDNRDAT
                     WAAVEAAL"
BASE COUNT          607 a          555 c          518 g          481 t
ORIGIN      
        1 gtcatctacc cggcccccta aggtccacga caacctgcgc acgcagcctc actacaggcg
       61 cgtgcgcttt tcctatctgg acaaactacg ctagatttgc taaactcttc gttagcgtta
      121 ccagagacag tggcagggaa accttaatca tgccaccgag gcgacaatac ttacaacaag
      181 tattttctcg tatctggccg tttggccaga ttttctgtaa cgatacatta acaattcggc
      241 tttatttaaa atcaactcta acgacgaagg atccaaatat ggcaatttct cgtgataaga
      301 aaactgagtt ggtcaaagaa ctatccgata tcctagcttc ggctaaactg accgccgccg
      361 ccaagtacgc cggcctgagc gttaccgata tgcaggaatt gcgccgcact gcacgcgagt
      421 ctgacgtcac catcaaggtc gtcaaaaacc gcctgcttcg cgtcgctatg gagcaatccg
      481 ctaagttcaa gagtgctgat aagtcactcc tggaaggcca gttactctac gcctacagtg
      541 agactgatga agtggccccg gctcaggtct tggctaaatt tgccaaaacc cagccggaac
      601 tcgaattggt cgctggtttt gacgccgacg gcaaggtgct cgacaccgcc gcaatcaaag
      661 ccctggccga tctaccgagc aaggatcagc tacgtggcca agtggtcggc gttatcgctg
      721 caccgcttac ccagatgctt ggcgccctaa atggcgctca gcgcggattc gctcaagttc
      781 tgagccaaca ggccgaaaaa acttcggcct aactagacga tagacagtag atgctggata
      841 gtagaaatta cttcttacgt atctccatct ttcaatgtcc aacgtccaac atctaatcta
      901 taaggagata aattatggca gatgaagata agaaaatgcc tgagctaaat gcagctcaga
      961 agaaaatcgt tgacgagctc aacaagctct cagccctcga gctgagccaa atcgttaagc
     1021 acttagaaga cgagtggggc gttagtgctg ctgctccagc tgctgttgcc gtcgccggcc
     1081 cagccgctgg cggtgaagct gccgccgctg aagacgagaa gactgaattc accgtcactt
     1141 tgaaagaagc cggtgctcaa aaagtcgccg tcatcaaggc tgtcaaagaa ttgaccggtc
     1201 taggactagg tgaagctaag ggcttggttg acaacgctcc tagcgccatc aaggaaaaag
     1261 tctctaagga tgacgctgaa gccgctaaga aagccttgga agaggctggc gctaccgtcg
     1321 aacttagcta atttacaagt tctttaaagc cgaattgaac agttacaaaa tgcccgccgg
     1381 actctctggt gggcattttg catggacaaa agtcaaggct taagaccgaa aatacaacaa
     1441 gttctttaaa tactctgttg gctttgtgga taagttgatt gacaaaattt gagccaatca
     1501 gtataattca catcagttca ttaactaaat aaaaatcaga cagaaggaac tgcgagctat
     1561 gcctgaatta ccagagaaac aagtcaaacg acttcgatca ctcatcagcg aggcggagac
     1621 caatctagcg gccgccaaag aattattaat tagtgttatc ggcgaggagg ccgttagtca
     1681 tggtgctccc gaggacgtag ccggtaaggt tatcgagggt gtcttcgacg gccagagtat
     1741 ggtcggaccg gacggtaaat ctttccccgt accagctaac tacgcctcta agtccaagct
     1801 gatcgaaggc gacatcttga agctaaccat aaccgaagac ggcagcttca tctacaagca
     1861 gattggcccg gtcgctcgac accaagtcat cggcacactt gcccagcacg acggcgctta
     1921 ctatgtcgaa gttggtggta aagaataccg tgtcctgtta gctagcgtaa cctacttcaa
     1981 ggccaaggtc ggtgaccaag tctcgatcgt catcccagaa gacaaccgcg acgccacctg
     2041 ggctgccgtc gaagccgcct tgtagcagct ccgaacacct caaaagtaat taattcaata
     2101 caaaataaat ccggatttac tccggtttta ttttgtatgc taagggaaaa gccaagcgaa
     2161 g
//
LOCUS       DASSBW010000012         6097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_499953, whole genome shotgun sequence.
ACCESSION   DASSBW010000012 DASSBW010000000
VERSION     DASSBW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499953"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..783
                     /locus_tag="VFG56_00270"
     CDS             223..783
                     /locus_tag="VFG56_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622355.1"
                     /translation="MTENQERWHDDYRVVRSVEPFEVVVEAGDVEPIRAMVASKFFSV
                     AKSSTRRLDEFGDKTVGCISGGRLVGDDDAPRVFAGASLEHFSNMWRLVILRTITHDS
                     ALSRVAVRYFLESDGQNVFEAKSQARVMRGTKDIIIDEEFIEEEVRYSRLEYEKPLDK
                     VGCLRLQRQLARAVARLEILSGVQTA"
     gene            863..1768
                     /locus_tag="VFG56_00275"
     CDS             863..1768
                     /locus_tag="VFG56_00275"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6622356.1"
                     /translation="MQKIVPHLWFDKEAGEAAEFYTQIFPNSRVTLRQLIPDTPSGDA
                     EQVAFNLNGYEFMAISAGPYFKLNPSISFMVNFDLSNDKDAKEKLDELWNKLIDGGQA
                     LMELDEYPFSQRYGWVQDRFGVSWQLILGSSEGEPRPYIVPSTMFIHDNAGRAEEARD
                     FYLSVFDDAQAGTTFKYEPGTAPDDASKVAYEDFQLYGQWFAAMDAGREMHDFDLNEA
                     VSFMVNCETQAEIDKLWSKLSADPAAEQCGWLKDKFGVSWQIVPVALGEMMSQGSPDQ
                     VQRVTEAFLKMKKFDIAELERAYSG"
     gene            complement(1802..2029)
                     /locus_tag="VFG56_00280"
     CDS             complement(1802..2029)
                     /locus_tag="VFG56_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HET6622357.1"
                     /translation="MSLLDDIKAKLDTNQDGRVSRDDLEALKNGDQDRDTKIEELKSH
                     ADQTDDGKLSFDDIKNFDFGGMIDNLKKKVM"
     gene            2158..2889
                     /locus_tag="VFG56_00285"
     CDS             2158..2889
                     /locus_tag="VFG56_00285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HET6622358.1"
                     /translation="MKRSYLHAQHFLKSPRLVAELIGHSNIRKNDLVLDLGAGTGVIS
                     SVLSRRARRVVAVEIEAGALEQLRHNMKRFGNVEVVEQDLLAIVPPAEPYKIFSNIPF
                     NLSAQVVRKFTTEDRPPKSIYLIVQKQFARKLVPGDTHFTSELSAEIGPLWTVRVRKP
                     LKKSDFTPPPAVDTVLLEIKRREEPLMPLRQMNEYSQWIERCFAEQKYFVTLPLEKAD
                     VSRELRPSQLTLEQWLKLWSVVKSV"
     gene            2893..3399
                     /locus_tag="VFG56_00290"
     CDS             2893..3399
                     /locus_tag="VFG56_00290"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6622359.1"
                     /translation="MDETKLIILRGPSGSGKSTVAKRLQLEARKPLAVIEQDYYKLSL
                     FSYAAAGNLSELRREMLVDDALYILSRGYDVVVEGIMSRGKYSQALLDLVKRHNGSSF
                     VYYFDIPFDETVKRHSMRHKSAEFGADKMKEWFLEADHLGAKEEAVIIPEMTEEEVVD
                     LIKKQADI"
     gene            3861..4088
                     /locus_tag="VFG56_00295"
     CDS             3861..4088
                     /locus_tag="VFG56_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622360.1"
                     /translation="MKRTDRKRINVSLRIRRDFVRKSKKDTTKSTQILNRKRVTRYFM
                     TDNVWIDVSVWAGLASRNTMPYRGTTTLRRT"
     gene            4273..4692
                     /locus_tag="VFG56_00300"
     CDS             4273..4692
                     /locus_tag="VFG56_00300"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6622361.1"
                     /translation="MNKLVPYINFADQGQEAIEFYKSIFGGEAEIQLVKDSPAAGGMP
                     KEWSERIFHMYYKAGDIELMGSDVMSDQAGKVVGNVFSLALNCDSAEELQKYFDGLSD
                     GGKVVWPVRDSEWGDVFAQVVDKFGLQWMMNYHREEA"
     gene            complement(5054..5308)
                     /locus_tag="VFG56_00305"
     CDS             complement(5054..5308)
                     /locus_tag="VFG56_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622362.1"
                     /translation="MLLITHIIIALTSLVFATYLLIRPSQVKLNMNYGLIAATIASGS
                     YLIVTTGQHWLSACLSGLVYSVIAVSLSLAARAKLARQEA"
     gene            5360..5869
                     /locus_tag="VFG56_00310"
     CDS             5360..5869
                     /locus_tag="VFG56_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622363.1"
                     /translation="MAFLTGLECQPDFPREDLTETNADLLELMLANFDLVEAGHQQAE
                     LISWIYRVGHRSQSSASRRVIDSAAQLESFDYGVRTFETASSLVRATAGEFDELIVNH
                     NGVALAHAFSEDQLRDYIDGARDKFDSEMPKTVDVVREASSRLSISLAGYAIFGAALA
                     RQFELDAIA"
BASE COUNT         1576 a         1333 c         1656 g         1532 t
ORIGIN      
        1 ggattcgatg tccgacctga tcgtcgcctc attcggctcg ctagttacgt cttttttcgt
       61 ttatctcggc tataaatacg aaaagaaagc tgcactcagt gtcatgcgtc ggacggtaag
      121 atttatcagg aaccagcggt actgattcgc caagccagcg aataggtgta taatcttata
      181 aaactaatcg ggtggctata cttagccacc ttgttttaat ttatgacaga aaaccaagaa
      241 cgctggcacg atgattacag ggtagttcgc tcggtcgaac cgtttgaggt tgtggttgag
      301 gctggagatg tcgaaccgat ccgggccatg gtggctagca aatttttcag cgtggctaag
      361 tcatcgacca gaaggctgga tgagttcggt gacaaaacgg tcggttgtat cagtggcggt
      421 cggctcgttg gtgacgatga tgcaccgcgg gtctttgccg gtgccagtct agagcatttt
      481 agcaatatgt ggcggttggt catcttgcgt actataaccc atgattcggc cctcagccga
      541 gtggcggtcc gatactttct agaatccgat ggacaaaatg tatttgaagc caagagtcag
      601 gctcgagtaa tgcgtggcac caaagatatt atcatcgacg aagaattcat cgaggaagaa
      661 gtccgctatt cgagactgga gtacgaaaaa ccgctcgaca aagtaggctg cctccggctt
      721 cagcgacagc tagctagggc ggtggccaga ctggagatcc tgtccggcgt acaaactgcc
      781 taaaaaggga aataattcat tgcaaaaagc tctgcctcgg cagacaattg gtaattatac
      841 gttttaagcg agggaggggt ttatgcaaaa gatagttcca cacctttggt tcgataaaga
      901 agcgggtgag gcggccgaat tttatactca aatcttccca aactctcgag taaccctgcg
      961 ccaactgata ccggacacgc cgagtggcga tgccgaacaa gtagccttca atctaaacgg
     1021 ctacgagttc atggctatca gcgccggtcc gtattttaag ctcaatccat cgatatcgtt
     1081 catggtcaat ttcgatctgt ctaacgacaa agatgccaaa gaaaagctgg atgaattgtg
     1141 gaataagttg atcgatggcg ggcaagccct gatggagcta gacgagtatc cgtttagtca
     1201 gcgctatggc tgggttcaag atagatttgg tgtgagctgg caactgatac tgggaagctc
     1261 ggaaggcgaa ccacgaccat acatagtacc gtccactatg ttcatacacg ataatgccgg
     1321 tcgggccgaa gaagctcgcg atttttacct gagtgttttt gatgatgccc aggccgggac
     1381 tacgtttaaa tacgaaccgg gtacggcacc tgatgatgcc agtaaggttg cctatgaaga
     1441 cttccaactg tacggtcaat ggttcgccgc catggatgcc ggtcgtgaga tgcatgattt
     1501 tgacctcaat gaagcagtat ctttcatggt caactgtgag acacaggccg agatcgataa
     1561 gctgtggagt aaattgtcgg ctgatccggc ggctgaacag tgtggctggc tgaaggataa
     1621 atttggtgtg tcatggcaaa tcgtgccggt ggccctaggt gagatgatga gccaaggtag
     1681 tccggatcaa gtccagcggg taaccgaagc tttcctcaag atgaagaaat ttgatatcgc
     1741 cgaactggag cgggcttatt ccggttaagc ggggtagtct gaaggaagaa ggttatgtat
     1801 gttacataac cttctttttt aggttgtcta tcataccgcc aaaatcaaag tttttgatgt
     1861 cgtcgaagct tagcttacca tcgtcggttt gatcagcatg tgactttagc tcttcaatct
     1921 tggtgtcacg atcttggtcg ccatttttta gcgcttccaa gtcgtctcta ctaacccgac
     1981 catcttggtt agtatcaagt ttcgctttga tatcatctag taaactcata aagatcctcc
     2041 ttcttagcga tcgttattat gaaggaccat agtcactcag tctgtgaaaa tttatataaa
     2101 taccgctagt agtggcgact tcatctgtat atcgatgtgg gttggtacaa tgaatcaatg
     2161 aaacgttcgt atctgcatgc ccaacatttt ctgaaaagcc cgaggttagt agccgagctg
     2221 atcggtcata gcaacataag aaagaatgac ttggtcttgg atctgggtgc tggcaccggc
     2281 gttatttcaa gtgtgttgag tagacgcgca cgccgggtgg tcgcggtcga gattgaagca
     2341 ggcgcgctcg agcaactacg ccacaatatg aagcggtttg gtaatgtcga ggtagttgaa
     2401 caagatcttt tggcgattgt gccaccagcc gagccctaca aaattttttc aaatataccg
     2461 ttcaacttga gtgctcaagt ggtccgaaaa ttcactaccg aagacaggcc gcctaagtct
     2521 atctacctga tcgtgcagaa gcaatttgct cgcaagctgg taccgggtga tacgcacttt
     2581 acatccgagc tgtcggccga aatcgggccg ttgtggactg tacgggtacg aaaaccgctt
     2641 aagaaatctg acttcacgcc accgccggcc gtcgacactg ttttgcttga gatcaagcgc
     2701 cgtgaagaac cgcttatgcc gcttcgacag atgaatgagt atagccagtg gatcgaacgc
     2761 tgcttcgcag agcagaaata tttcgttaca ttaccgttag agaaagctga cgtctcccgt
     2821 gagcttcgac cgtcacagtt gacgctcgag cagtggctta aactatggtc ggtggtaaaa
     2881 tcggtataac tcatggacga aactaagcta attatcttgc gtggaccttc cggttcgggc
     2941 aagagtacgg tagccaagcg attacagcta gaagctagaa agccgctggc tgtgattgag
     3001 caggattatt ataagctgtc cttgttttct tatgctgcag ccggaaacct ttcagagctc
     3061 cggcgagaga tgctggtgga tgatgcacta tatatcttga gtagaggtta tgatgtcgta
     3121 gttgaaggta ttatgagtag gggtaagtat agccaggccc tacttgatct ggtaaaacgc
     3181 cacaacgggt cgagctttgt ttattatttc gacattccgt ttgacgagac ggttaagcgt
     3241 cactctatga ggcataaatc ggctgagttt ggtgctgata agatgaaaga atggtttttg
     3301 gaagccgacc acctcggtgc taaagaagag gctgtaatta taccggagat gacagaagag
     3361 gaagttgtag atctgataaa gaaacaggcg gatatataag aggcaagaag aaatcgaagt
     3421 taaattcttg aatatagatt ttggcgatat ccgcactaaa ctggaggcgg ctggcggcaa
     3481 actgatcaat ccgatgcggc tgatgcgacg agtgactatc cataatgatg ctagggtagc
     3541 gggttaattt gtgtcagaat atgatatata aggtttgaca agataactgc agtatgtcag
     3601 agtatggact gtcgtcttcg catcgggtga agacgcatgc agaggaagga agtgcgatgc
     3661 gctttcggaa tgcaatgatc gcagccctcg caagtctgat gatgtcattc ggactggcga
     3721 tggtgagcgc accagccgca caggcggcga gtgcgtgtag cggcgtcgat acgccgtggg
     3781 atcttcggga gacggtccgt gatggcaagt acgtcatcag gaactactcc gcccgctact
     3841 acacctgttc caaggtggtc gtgaagcgga cggacagaaa gcggatcaac gtctcgctgc
     3901 gaattcgacg cgacttcgtc cgcaagagca agaaggacac caccaagtcg acgcagatct
     3961 tgaacaggaa gcgcgtgacg cgttacttca tgaccgataa cgtctggatc gacgtaagcg
     4021 tgtgggctgg tcttgccagc cggaacacca tgccttaccg aggtaccact acgcttagac
     4081 gcacctgacc tcacgcttcc cgtggaatcg cgacttctca tgtccgattc cacgggaagc
     4141 acatcattct cagcgccttc aaaaggcgtt tttattttgt ctacgaatta atagaccggt
     4201 aaactcacct gcgaagtagt taaacttacg gcgacgggtc tataattaga catataaaca
     4261 aggaggtgct ttatgaacaa gttagtaccg tatatcaact ttgccgacca gggccaagag
     4321 gcgatcgagt tttataagtc aattttcggt ggcgaagccg aaatccaact agtcaaagac
     4381 agcccagccg ccggagggat gccgaaagaa tggagcgagc ggatctttca tatgtactat
     4441 aaggctggcg acatagagct gatgggcagc gacgttatga gcgaccaggc cggtaaggtt
     4501 gtcggcaacg tattttcgtt ggcgctcaac tgcgatagtg ccgaagagtt gcaaaaatac
     4561 tttgatggct tgtccgatgg cggcaaagtg gtctggccgg tccgggatag cgaatggggt
     4621 gatgtcttcg ctcaggtagt tgataagttc ggcctgcagt ggatgatgaa ttatcaccgc
     4681 gaagaggcat aggaaaagcc gcgctctgga ttgcaaagcg cggctaatgg tggctcacct
     4741 ccaagtgtcg ggcgagacat tggagtcggg gtcgagccgg tcgaactgta agttttcgta
     4801 ggcgatcata ccaggatcaa cgccttgcgt ttgtcggatg ttggcgcaga atatatgggc
     4861 aatctgaccg cgggaacgtg gatggccact tcttaggcgg cgcatcgttt ggtcatctat
     4921 cggtaggcaa gtcgcccagc cgctatcgga gcggacaagg tatacggccg tctggtcgct
     4981 tgtcaatgct caccctaact tctcggaaat gagatggccg gtacctccgg ctagatacta
     5041 attatagcaa tttttaggct tcttgacgag ctagcttggc cctagcggct agcgacaagg
     5101 aaacggctat aacagagtaa acaaggccgc ttagacaggc tgataaccag tgttgaccgg
     5161 ttgtaacgat caagtagctg ccactagcta tggtggcggc aatcagacca tagttcatgt
     5221 tgagtttgac ttgactaggt cgaatcagga ggtaagttgc aaagactaaa ctagtgagag
     5281 cgatgatgat atgtgtaatt aaaagcataa ggccattata tcacgttgac atatatgcag
     5341 atcgttttac aataccgcta tggcattcct gacaggccta gagtgtcagc cagactttcc
     5401 aagagaagat ttgaccgaga ccaacgccga tttactagaa ctgatgttag ctaacttcga
     5461 tctggtcgaa gccggccacc agcaagctga actaatctca tggatttacc gggttggcca
     5521 tcgctctcag agtagcgcta gtcgacgagt tatcgatagt gcggctcagt tggaatcgtt
     5581 tgattacggt gttaggacat ttgaaactgc ttcatcattg gttagagcta cggcgggtga
     5641 attcgatgag ttgatagtaa accataacgg cgtggcctta gctcatgctt tcagcgaaga
     5701 ccagttgagg gactacatcg atggagcaag ggataaattt gattccgaaa tgcccaagac
     5761 cgtcgatgta gttcgagagg cgtctagtcg tctcagtatc agcctagctg gttacgccat
     5821 cttcggagca gccctagctc gtcaatttga gctcgacgcc atagcctaaa cttcaaaatc
     5881 ccggagtact cggtctaagt cgtggccccg ctgtctgtga attcaggcag cggggcatat
     5941 taatttcata tatcctccgg tcgataacgg cggggtagta tgcgcttgaa gcaggtgaca
     6001 ttttcaacgg taaatgtttg cggtagctca acggcttgca caatttttga cccgatcgcg
     6061 tcaagattga tgcatttggg ttcatatttt tgagcaa
//
LOCUS       DASSBW010000013         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_466279, whole genome shotgun sequence.
ACCESSION   DASSBW010000013 DASSBW010000000
VERSION     DASSBW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466279"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..521
                     /gene="rpsR"
                     /locus_tag="VFG56_00315"
     CDS             321..521
                     /gene="rpsR"
                     /locus_tag="VFG56_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET6622364.1"
                     /translation="MPKRFKKDAPAYFDYRDVKTLQRYINSYGQIEPSTKTGLNPRQQ
                     RSLASAIKRARHLALLPFVALG"
     gene            612..1172
                     /gene="efp"
                     /locus_tag="VFG56_00320"
     CDS             612..1172
                     /gene="efp"
                     /locus_tag="VFG56_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET6622365.1"
                     /translation="MYQPTDLKKGTVIQLDGQPYKVTDYSQKVMGRGGSIVNLKLKNL
                     ITGAAIPKTFKGADKIEPAEVTGRKAQYLYTDGADYHFMDPSSFEQFQLSADTVEDIR
                     GYLKEGDEVNLESFNGQIISVEIPKALYFEVTYTENAVKGDTTSSIQKDAKLETGLTI
                     KVPAFIKVGDIIKVDTRDASYIERKK"
     gene            1218..1745
                     /locus_tag="VFG56_00325"
     CDS             1218..1745
                     /locus_tag="VFG56_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622366.1"
                     /translation="MSRIEAGDRMVVSFVEPVETRTFRADDWPYHVALLPWYRIDDRQ
                     LGNWQTQLRRIADTSEAFETEVGEEDYFGKRHNILGRRLAQSAFRSLHYRLFNAVSCE
                     KNGVGGDFASPNEVGKGYDPYIAQRFSQLLEEGQVIRIASLSEIVKIDAGLGEYDLEV
                     VSQHQLKQGKGETAA"
     gene            1729..2505
                     /locus_tag="VFG56_00330"
     CDS             1729..2505
                     /locus_tag="VFG56_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6622367.1"
                     /translation="MKQRLDLVLVGRGLVETRSQAESWIRLGRVSVADRIVKKPGFFV
                     ADNAVIKLKAEEQYVSRAGLKLASVASVLGINFDDKVVLDVGSSTGGFTDYALKHGAQ
                     KVIAVDVGTDQMHPSLRGDDRVELHEKTNILDVGRLAVSDSNNTVAQAPNSYSLSSIP
                     DIVVMDLSFVSLRHILPHIAGLCGPRTQVVAMVKPQFEAGKHQVNKGVIKNNAVRRQI
                     LKEFEAWVKDYFMICDKADSQVHGASGNQERFYLLTPLTK"
     gene            2649..>3058
                     /locus_tag="VFG56_00335"
     CDS             2649..>3058
                     /locus_tag="VFG56_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622368.1"
                     /translation="MAGMPSLRTKLRPRSWLSAITALIIALAGVGIAQTAAQATSRPI
                     MSITEVELTCSDAGGSAAILEFYVNNSSPDAGVYDYETLINDRVRSGTDYFDANDSHL
                     VREFFGDDGFTGLTGEESSYTIKATGSTEPGGPT"
BASE COUNT          783 a          709 c          815 g          751 t
ORIGIN      
        1 taagcgttct aaagtcgagg ttgtggttga ggacttcaac ttcgtcgata gccaaggtgg
       61 cggtggtaat gctggcaaca acagtagcga cagcaagcct gcgcctaagt cagagtcagc
      121 cgccgaagac gttgatgata agccgattga tttaagcgaa atcccattct aagtatttct
      181 cgatggcacg ctactttgtt gcagcttcca atcctcgctc gcagtattat tcatacagct
      241 cgctcgggtt tcggctgcgc cgcgtagcgc acacatcggc taaatactta gctaactcga
      301 ttaattaagg agaatacttt atgccaaaac gatttaaaaa agacgcccca gcttattttg
      361 actaccgcga tgtcaaaacg ctgcagcgct acatcaacag ctacggtcaa atagaaccta
      421 gcaccaagac tggcctcaat ccgcgccagc aacggagcct tgctagcgct atcaagcggg
      481 ctcgtcatct ggcattgctg cccttcgtag cgctgggtta agtagacgaa tagacagtag
      541 agggtggacg tttgaggtcc tctaccgttg cttaagtcta gcttctatca tctaacgtcc
      601 aacgtctaat catgtatcaa ccaaccgact taaaaaaagg caccgtcatc cagcttgatg
      661 ggcagccgta caaagttacc gattactcac aaaaagtcat ggggcgtggc ggttctatcg
      721 tcaacctgaa gctcaagaac ctgatcactg gagcggccat cccgaagacc ttcaagggag
      781 ccgacaagat tgagccagct gaggttaccg gtcgtaaggc tcagtacctc tacaccgatg
      841 gagccgacta ccatttcatg gatccgtcaa gcttcgagca attccaatta tcggccgaca
      901 cggtcgagga cattagaggt tatctgaaag aaggcgatga agtcaatctt gagagtttca
      961 atggtcagat tatcagcgta gaaattccaa aagcgctcta ctttgaggtg acctatactg
     1021 agaatgccgt caaaggtgac actaccagct ctatccaaaa agacgccaaa ttagaaaccg
     1081 gcctgactat caaggtgcct gcatttataa aagtcggcga tatcatcaag gtcgacactc
     1141 gcgacgccag ctacatcgaa cgcaagaagt agcgcttaag ctagacaagc cgaatcaact
     1201 acagccataa tagcggcatg agtagaattg aagctggtga tcggatggtg gttagtttcg
     1261 tcgaaccggt cgaaactagg actttcaggg ccgatgattg gccttaccat gttgcattat
     1321 tgccgtggta ccgaattgac gaccgtcaat taggtaattg gcagactcaa ctacggcgaa
     1381 tcgccgatac atccgaggcg tttgaaacag aagttggtga ggaagattat ttcggtaaac
     1441 gtcacaatat cctggggcgt cgtttggcgc agtcggcctt tcgttcgctg cattatcgtc
     1501 tgtttaacgc agtttcatgc gagaagaacg gtgtcggtgg tgattttgcc agtccgaatg
     1561 aagttggcaa gggttatgat ccgtatattg cccagcggtt tagtcagcta ctggaagaag
     1621 gtcaggttat tcgaatagct tccttaagtg aaatcgtgaa gatcgatgct ggtttaggtg
     1681 aatacgatct agaggttgtc tcccagcatc agctgaagca gggtaagggt gaaacagcgg
     1741 cttgatttag ttttggtagg gcgcggtttg gttgagacta ggtcccaagc cgaaagctgg
     1801 atccggttag gtcgggtcag cgtggccgat cggatagtca agaagcccgg ctttttcgtg
     1861 gccgataatg cggtgattaa gcttaaggca gaggagcaat atgtctctag ggccggcctg
     1921 aaactagcca gtgtagcaag tgttttgggt attaatttcg atgataaagt cgtgttggat
     1981 gttggttcta gtactggcgg ttttactgat tatgctctca agcatggtgc ccaaaaagtc
     2041 atagctgtcg atgtcggcac cgatcagatg catcccagtc tgcgtggtga tgacagggtg
     2101 gaattacatg agaaaaccaa tatattggac gttggacgct tggcggtgag cgacagtaat
     2161 aacactgtag cccaagctcc caatagctat agtctatcgt ctatacccga tatcgtggtg
     2221 atggatctta gttttgtatc actgcgccac atattgccac atatcgctgg gttatgtggg
     2281 ccgcgaactc aagtcgtagc catggtgaag cctcagtttg aggccggcaa acatcaagtc
     2341 aacaaaggtg ttatcaaaaa taatgccgtt cggcgacaga tccttaagga gtttgaggct
     2401 tgggtaaaag actatttcat gatctgcgat aaggccgata gccaggttca tggcgcctcg
     2461 ggcaatcaag aacggtttta tctgcttact cccttgacta agtagtcact atattttaca
     2521 aaactgatta taggtggtat aattctaaac tagcttctgt gagaatttca ggggtgtacg
     2581 cggttcgccg cggcacgttg gtggcagccg ccatcgacgt ttgacaaatc ggttctacaa
     2641 ggaaggaggt ggctggtatg ccatctctca ggacgaagct taggcctcgg tcttggctca
     2701 gcgctatcac ggcgttgatc atagctctag ccggcgtggg tattgcccag acggcggctc
     2761 aggccacgtc gagacccatc atgtcaatca ccgaggtcga gctgacctgt agcgatgctg
     2821 gtggcagcgc cgccatactc gagttctacg tcaacaacag ctcgcccgat gccggtgtct
     2881 acgactatga aacgctgatc aatgatcggg ttcggtctgg cacggactac ttcgatgcca
     2941 acgacagtca cttggtcagg gagttcttcg gcgacgacgg ctttaccggg ttgaccgggg
     3001 aagagtcgag ctacaccatc aaggcgaccg gttctactga gcctggtggg ccaaccga
//
LOCUS       DASSBW010000014         8214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1704464, whole genome shotgun sequence.
ACCESSION   DASSBW010000014 DASSBW010000000
VERSION     DASSBW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704464"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VFG56_00340"
     CDS             <1..953
                     /locus_tag="VFG56_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622369.1"
                     /translation="QATKKTQSTTFTATGKKNVGDKATGTVNFSNSNPSAVTLSAGTE
                     LTSSSGLVFLTDSTVTIPGATLDFGCSGYLCPGKASVGVTAAERGSKYNGASGSVSGT
                     PDGVSGSFADATTGGTDETATVVSEDDVEKAKQEIKKDDQEAIRQELAKQFEGDYVII
                     NESFTAKTGDPAVSPAVGEKAETAQLAVETTYTVYAIKRSDLAAIFDAYLKTQMSDPS
                     NQKIYASGDQEVSFSEWDKKDDEFTVRANATGQIGPEIDENQLKQDSEGKMSGEVQQE
                     VGSLSGVQDVKVNLSPFWVSHMPEPDRVTIKFILQDESNQ"
     gene            937..1350
                     /gene="ruvX"
                     /locus_tag="VFG56_00345"
     CDS             937..1350
                     /gene="ruvX"
                     /locus_tag="VFG56_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET6622370.1"
                     /translation="MSQTSNHILALDVGEKRIGLAQADTHTRFPFPLTTLTVDDMVIS
                     RLREIINEIAPVCLVVGYPRNQSGQPTQQTASVEAFAEELKAFRIPIVWQDESLTSVL
                     AEDYLKSHKKTYTKADIDAHAAAIILGDYLEANYA"
     gene            1343..2449
                     /gene="mltG"
                     /locus_tag="VFG56_00350"
     CDS             1343..2449
                     /gene="mltG"
                     /locus_tag="VFG56_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET6622371.1"
                     /translation="MPKFSLPWQQKSYENGLQLNTRRYLDWRFWFLVIAATLALTAGI
                     FGLVYFHNLGAKDSTDDAETLLIEVSPGDTAGTIANELDEAKLIRSQWSFSLYARLNH
                     SVFLPGRYMISSAQTVADIIGVMAKGKSSVFNVTFIAGMTLDQLKQRLISQYGYQAAE
                     IDRAFKADYHNPLLVDKPAHASLEGYIFPETFKVNVDDSLQALLERDFNELYSKLTTD
                     HLIAKFKQHDLTIYQALTLASIVQKEASSPRDQRLVAQVFLKRLKEDKPLGSDVTALY
                     GAEQAGVNLPTDPAAAAAIAVSYDSPYNTRLHKGLPPGPISNMEYSALQAVANPAKTD
                     YTYFIGGDDRKMHYARTLAEHNANIERYCQISCR"
     gene            2580..3773
                     /locus_tag="VFG56_00355"
     CDS             2580..3773
                     /locus_tag="VFG56_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002385557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET6622372.1"
                     /translation="MTAATRLVTGVTEKHISTNSSVKAESSNKLRQLPRPLAPAKRRK
                     SGFEWLSRSAIYISVAILMISLVSIGYQSPINAEKLDSGKAAQAVEATTPSVDQVTAA
                     NLAASIAEYSDLAVASKVSNVAISLNTKQQLSQTNNNFLSKPQIIEADQTGIIRTYHT
                     HAGDTVSQLAKRYGVSERSIKWSNDLTSDALDAGDTLLIPGTNGFIYEVESGDTVAKL
                     AKHYQASKQRIITYNSLEKKGLKKGQDIVIPDGILPSSERPKAYNPTASSMSSGGGSV
                     ASTSGYHSAAVVGNRYAYGYCTWYAYNRRAQLGKPVGSFWGNAATWASYARSSGYTVN
                     HRPAPGAVMQSAGGWGGYGHVAVVESVNSDGSITVSEMNYAGWGIVDHRTISAGQASS
                     YNYIH"
     gene            3966..5261
                     /gene="obgE"
                     /locus_tag="VFG56_00360"
     CDS             3966..5261
                     /gene="obgE"
                     /locus_tag="VFG56_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET6622373.1"
                     /translation="MFADTALVLVSAGKGGDGAVSFRHEIYVDKGGPDGGDGGKGGDV
                     IFEADENLNTLSHFRHRQELKADNGSPGAKRQKHGKSGQDLVVRVPVGTMIYRDDRLL
                     VDLNSHGQNQVVARGGDGGFGNAHFKSSTRQTPRVAELGEPGDSLEAKLELKLLADVG
                     LIGFPNAGKSTFLSVVTNARPEIADYAFTTLTPNLGVADIDDTSLLIADIPGLIEGAA
                     EGKGLGDQFLRHVERTAVLLHLIDIYQDDLVASYQTIRKELADYDPKLAELPEIVALT
                     KSEGLDDEMINHRLAELQAVVEAKTPLMAISARAHQGLEVLLRELVEQVRLGRQNQTV
                     EVDETDDTPLISLNAEAHQSSWQVEKTEQGYRISGQKIEKFARRTDFSNFHSVNRLRD
                     IMQKMGIGHELRRQGAVPESKLTFGSQSQSITLQEIADD"
     gene            5286..6698
                     /gene="sbcB"
                     /locus_tag="VFG56_00365"
     CDS             5286..6698
                     /gene="sbcB"
                     /locus_tag="VFG56_00365"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HET6622374.1"
                     /translation="MSQTLFFYDLETSGLNSRSGRIMQFAGQRTDMDLKPLGQPVNLL
                     VKLSDDILPDPGAIMVTGITPQKTVEEGYSEPEFCKFLDQEVFTPGTVTIGYNNVRFD
                     DEFIRHTMWRNFYDPYEWAWSDERSRWDVLDVVRLVRALRPDGINWPVNADGKPVNKL
                     EMIAKANNLTHTRAHDALSDVEVLIDLTRLIKTKQPKMFDYLFNLRNKKAVSKLVGLD
                     EPKAFVYASGRYDDRFEKTTVAYPISLGSKPNSLLVYDLRYDPTPFAKATPRALSEVM
                     FASKEQRADPSFVRLPVKELTINKCPAVAPLGVLDDDAQKRISLKLDTIKKNLDKLVK
                     LPDFGDRVREAYEMREPYPEANDVEAKLYDSFTPDQDKPRLSAIRAASADELADFHPN
                     FADERLGELLLRYKARSFPTSLSQAEQEAWDEYRQNKLAAQVEPYMKSLAVQAEKGGD
                     SFLIDELRLWVESILPVEVD"
     gene            complement(6678..7004)
                     /locus_tag="VFG56_00370"
     CDS             complement(6678..7004)
                     /locus_tag="VFG56_00370"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HET6622375.1"
                     /translation="MVKLKENWRRLPTKPRQTLVLLAGISLVILAGFVGAVPGPGGVF
                     VFALGVAILGSEFEWAERFGKWVFGLIRRFNDFLKAHHMISLVFGTVVVAWMVYVIIW
                     LVDLNR"
     gene            7065..>8214
                     /locus_tag="VFG56_00375"
     CDS             7065..>8214
                     /locus_tag="VFG56_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6622376.1"
                     /translation="MSKAIKVVGAREHNLKDISVDIPRDKFVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGIMDKPDVDQIDGLSPAISIDQKSASRNPRSTVATV
                     TEIYDYLRLLFARVGVPHCPIDGQVVKRQTTETIINSVMELGDDKRLIILAPIVSEKK
                     GEFAHIPEKYQKAGFARVRVDGVIYALDEWPQLDKNYKHTIELVVDRLVLNPSARGRV
                     AQSVEQALEIADSVVAVLDADSDELHIFSQRYACPDHPDLAIPELEPRTFSFNSPFGA
                     CPVCTGLGFRQEVDPELVFNPNLTIMEGAIRPYNRINSDAWYTKKLKALGEAHNFSVH
                     VPVKQLTAEELDKILYGTGAQKYTVELGNGRHFQTTYEGVIPNLERRHR"
BASE COUNT         2159 a         1995 c         2047 g         2013 t
ORIGIN      
        1 ctcaggccac caagaagacc caatcgacga catttacggc cacgggtaag aagaatgttg
       61 gcgataaagc aactggaact gttaactttt ctaatagtaa tccaagtgcc gtaacgttaa
      121 gtgctggtac agaattaaca agcagtagtg gcttagtctt tttgactgac agcaccgtaa
      181 ctatacctgg cgccacgtta gactttggtt gtagtggcta cctctgtcca ggtaaggctt
      241 ctgttggcgt aacggccgct gaacggggct caaagtataa tggagcttct ggtagtgtga
      301 gtggtacgcc ggatggtgta tcaggttctt tcgctgacgc gacgacaggc ggtaccgatg
      361 aaacagctac tgttgtttca gaggatgatg ttgaaaaagc taagcaagag atcaaaaaag
      421 acgatcagga agctatccgc caagaactgg ctaagcaatt tgaaggagat tacgtcataa
      481 tcaacgaaag ttttaccgct aaaactggtg atccggcggt ctctccggcg gttggcgaaa
      541 aagccgagac cgcacaactg gcggtcgaaa caacttacac cgtttatgct ataaagcgat
      601 ccgatttggc ggcgatcttt gacgcttact taaagactca gatgtccgat ccgagtaacc
      661 aaaaaatcta tgccagcggt gaccaagaag tcagcttctc agagtgggac aaaaaagacg
      721 atgagttcac ggtacgcgcc aatgctactg gccagatcgg ccccgaaatc gacgagaatc
      781 aattgaagca agactccgaa ggcaaaatga gcggtgaagt ccaacaagaa gtcggttcac
      841 ttagcggcgt tcaagatgtc aaggttaatc tatcaccatt ttgggtcagt cacatgccgg
      901 agcccgaccg ggtgacgatc aagtttatct tgcaggatga gtcaaaccag taaccatatc
      961 ctggcgcttg acgtcggcga gaagcggatc ggcttggctc aagctgacac tcacacgcga
     1021 ttcccttttc ctctgactac tttaacggtt gacgatatgg tgatcagtcg tttgcgcgaa
     1081 attatcaatg agatagcgcc agtctgttta gtggtaggct acccaaggaa tcagtcgggt
     1141 cagcctactc aacaaacagc tagcgtcgaa gcttttgccg aagaactaaa agcttttcgc
     1201 ataccgatcg tctggcagga tgaatcattg acttctgtat tggctgaaga ttatctcaaa
     1261 tctcataaaa agacctatac caaagccgac atagacgctc acgctgccgc tattatccta
     1321 ggcgattact tggaggcgaa ctatgcctaa gtttagcttg ccgtggcaac aaaaatcata
     1381 cgagaatggt ctccagctga acactaggcg ttatctagat tggcggttct ggttcctagt
     1441 aattgcagca actttggcgc ttaccgccgg tatatttggc ttggtctact tccacaatct
     1501 cggcgccaaa gactctaccg atgacgccga gactctgctg attgaagtct ctcccgggga
     1561 tacggccgga actattgcta acgagttgga cgaagctaaa ttgattcgca gccagtggtc
     1621 attcagtttg tatgcacgtc taaaccactc ggttttcttg cccgggcggt acatgattag
     1681 ctcagcacag acggtagccg atataattgg tgtcatggca aagggcaaat ccagcgtttt
     1741 caacgttaca tttatcgccg gtatgacgct tgatcaactc aagcagcgcc ttatcagtca
     1801 gtatggctat caggctgctg agattgaccg ggcttttaag gctgattacc acaatcctct
     1861 actcgtcgat aagccggccc atgccagtct agaaggatac attttccctg agacgtttaa
     1921 ggtcaatgtt gacgatagtc tgcaggcgct tttggagcga gacttcaatg agctttattc
     1981 gaaattgacc actgaccact tgattgccaa atttaagcaa cacgacttga cgatttacca
     2041 agctttgacg ctagcttcga tcgttcaaaa ggaagcaagt agtccgcggg accagcgtct
     2101 agtagcccag gtctttttga agcgactcaa ggaagataag ccgcttggat cggatgttac
     2161 cgcactgtac ggcgccgagc aggctggcgt taacttgccg actgatccag cggctgcggc
     2221 tgcaatagcg gtgagctatg actcgcctta caatactcgt ttgcataaag gtctgccgcc
     2281 tgggccgatt tccaacatgg aatacagcgc cctgcaagcg gtggcgaacc ctgccaagac
     2341 cgactacact tactttatcg gtggtgatga ccgaaaaatg cactacgccc gtaccttggc
     2401 tgagcacaac gccaatattg agcgatattg tcagattagt tgtcgctaaa atgcatccta
     2461 gacaataaat tgacactatt ttccatagtt gatacaatag ttgtgctaat tagcggtgtt
     2521 tatggtggta atttgcccta aacgcttaag ctaatcgcct accgttgtcg gtttaataga
     2581 tgacggcagc gactcgtctg gtaacaggag tcacggagaa gcatatcagt accaattcat
     2641 cggtaaaagc cgaaagttca aacaaactta gacaattacc gcgaccgcta gctccagcta
     2701 agcggcgtaa atcaggtttc gagtggctat ctcggtcggc tatctatata tcggtagcaa
     2761 tcctgatgat tagcctggta tcgatcggtt accaatcgcc gattaatgcc gaaaagctcg
     2821 atagtggcaa ggcggctcaa gccgtcgagg ctacgacacc atctgtcgac caggtgactg
     2881 cggccaattt ggcagcttct atcgccgaat actctgatct tgccgtggca tccaaggtgt
     2941 ctaacgtggc aatctctttg aataccaagc agcagttgtc tcaaaccaat aacaacttct
     3001 tgtctaagcc tcaaattatc gaggctgatc agacaggtat catccggact taccacactc
     3061 acgccggcga taccgttagt caattagcta aacgctatgg tgtgagcgaa cggagtatta
     3121 agtggtccaa cgatctgacg tctgatgctc ttgatgctgg cgacactctg ttaatccctg
     3181 gcaccaacgg ctttatctat gaagtcgaat ctggcgacac tgttgctaag ctggctaagc
     3241 actaccaggc tagcaagcag cggattatca cctacaacag ccttgagaaa aaaggcctta
     3301 agaaaggtca agatattgtc atacctgacg gtatcctacc aagtagtgag cggccaaaag
     3361 cttacaatcc aactgcctca tctatgagtt ccggtggcgg ctcggtagcc tcgactagtg
     3421 gttaccacag tgctgccgta gtcggcaacc gctacgccta tggctactgt acttggtacg
     3481 cctacaatcg tcgggctcaa ctcggtaaac cggttggtag cttctggggt aacgccgcta
     3541 cctgggccag ctacgcccgc tcaagtggtt acacggtcaa ccataggccg gcaccagggg
     3601 ccgttatgca atcggctggt ggctggggtg gctatggtca cgtcgccgtc gttgaaagcg
     3661 taaactctga cggtagcatc accgtctccg aaatgaacta cgctggctgg ggtatagtcg
     3721 accaccgcac catttcggct gggcaagcct ctagttataa ctacatccac tagagtcagt
     3781 gtagcgagcg atctacagcg tcaaagcctc cgttactcct tcactgtatg ttagatacag
     3841 cccagtccgt tactcggcct ttccttgtat atcatttcaa ctacaccact tcttggcgtc
     3901 atatgattaa aaactcaacc ctcgaggcta tcctaacagg ggtagatttg atataattta
     3961 actatatgtt tgcagatacg gctttagtat tggtttcagc tggtaagggc ggcgatggtg
     4021 ctgtcagttt cagacatgaa atatatgttg ataagggagg gccagatggc ggtgacggtg
     4081 gcaagggtgg cgacgttatc tttgaggccg atgaaaatct caatacccta agtcactttc
     4141 gtcaccggca agagctgaaa gccgacaatg gttctccggg agctaagcgt cagaaacatg
     4201 gcaaaagcgg ccaagatttg gtggttaggg tgccggtagg taccatgatc tatcgcgatg
     4261 atcggttact ggttgaccta aacagccacg gtcagaacca agttgtcgct cgtggtggtg
     4321 acggcggttt tggtaacgct cacttcaaga gctcgacccg ccagacacct cgtgtggccg
     4381 aattaggcga gccgggtgac agcctggaag ctaagttgga actcaaatta ttagctgacg
     4441 tcggtttgat tggttttcct aatgctggta agtcaacctt tctttctgtt gtcaccaatg
     4501 ccaggcccga gattgccgat tacgccttca ccacactgac acctaatctc ggtgtggccg
     4561 atatcgatga cacaagcttg ttgattgctg atatccccgg cttgatcgaa ggtgccgccg
     4621 aaggcaaggg cctgggcgat cagttccttc gtcacgtcga gcggactgcc gtgctgcttc
     4681 acctaatcga tatttatcaa gacgatctag tcgccagcta tcagacgatc cgcaaagaat
     4741 tggccgatta tgatcctaag cttgctgagc ttccggagat tgttgctctg acgaagtccg
     4801 aagggcttga tgatgaaatg attaaccatc gtttagccga actccaagct gtagtggaag
     4861 ccaaaacacc gctgatggca atcagtgctc gagctcacca aggcctagag gtactactgc
     4921 gtgaattggt cgagcaagtc agacttggtc gacaaaatca aacagtcgag gtcgatgaga
     4981 ccgatgacac gccgttgatc agtttgaatg ccgaagctca tcagtccagc tggcaggttg
     5041 agaaaaccga acagggttat cgtataagcg gtcagaagat tgagaaattc gctcgtcgta
     5101 ctgatttttc taattttcac agcgtcaatc gattgcgcga tatcatgcaa aagatgggta
     5161 ttggccatga actccggcga cagggagctg tgcccgaatc aaaactaact tttggcagcc
     5221 aaagtcaatc tatcaccttg caagaaatag ccgacgacta aacggtttgc tatgctagtt
     5281 ataagatgag tcagacctta tttttctatg acctggagac cagcggcctc aattcgcgat
     5341 ctgggcgaat tatgcaattt gccggtcaac ggaccgatat ggacttaaag ccgctcggtc
     5401 aaccggttaa tctattggta aaactatccg acgatatcct accggaccca ggtgctatca
     5461 tggttaccgg tattacgcca cagaagacgg tcgaggaagg ctatagcgaa cctgagttct
     5521 gtaaattctt agatcaagag gtatttacac ccggtaccgt cactatcggc tacaacaacg
     5581 tccggtttga tgatgagttt attcgccata cgatgtggcg gaacttttat gatccttacg
     5641 aatgggcctg gtctgatgaa cggtcacgct gggacgtgct ggatgtagta cgactggtgc
     5701 gagctcttcg acctgacggc attaattggc cggtaaatgc cgatggcaaa ccggttaata
     5761 aactagagat gatcgccaaa gccaacaacc tgacccacac ccgcgcccac gacgcattga
     5821 gtgacgttga agtactgata gatctgacta gactgatcaa aactaaacag cctaaaatgt
     5881 ttgattatct gtttaatttg cgcaataaga aagcggtctc taagctagtt ggcttggatg
     5941 aaccaaaagc tttcgtctat gcttctggcc gctacgacga taggtttgaa aagaccactg
     6001 tagcttatcc gatatcgctt ggatcgaagc ctaattcgtt actagtctac gacttaagat
     6061 atgacccaac gccatttgcc aaggctacac cacgggcatt gagcgaggtt atgttcgcct
     6121 cgaaagagca aagggccgat ccgagcttcg tgcgcttacc ggtcaaagag ttgacgatca
     6181 acaaatgccc ggcggtagca ccactgggag ttctagatga tgacgcccaa aaacgtatca
     6241 gtttgaaact ggacactatc aaaaaaaacc tagacaaatt ggtcaagctg ccggattttg
     6301 gcgatcgtgt gcgtgaggct tatgagatgc gcgaaccgta tcctgaggct aacgatgttg
     6361 aggccaaact gtacgatagc tttacgccag atcaggacaa gccacggcta agtgccatca
     6421 gggcggccag cgctgatgaa ttagccgatt tccatccaaa ctttgctgat gagcggctgg
     6481 gtgagttgtt gctgcgttat aaagctcgat cttttccgac cagcctcagc caagccgaac
     6541 aggaggcttg ggatgaatac cgtcaaaaca agctagccgc acaagtcgaa ccgtatatga
     6601 aatcattggc tgttcaagct gaaaaaggtg gtgatagttt tctgatcgac gaactgaggc
     6661 tgtgggttga atcgatctta ccggttgagg tcgactagcc agataatcac atagaccatc
     6721 caggccacca ctactgtgcc aaatactagg ctaatcatat ggtgtgcttt caagaagtcg
     6781 ttgaagcgcc taatcaaacc gaaaacccat ttgccaaatc gctcagccca ctcaaactct
     6841 gaacccaaga tggcaacacc gagggcaaaa acaaaaacac cgcccggacc cgggacggca
     6901 ccaacaaatc cggctagtat gaccaagctg atgccggcta gaaggactag cgtctgacgc
     6961 ggttttgttg gcaaacgccg ccagttttcc ttgagcttta ccatgtcatt attatagctg
     7021 ctatctcgac ataaactact agtaattgct ataatagttc ggttatgtca aaagccatca
     7081 aagtagttgg tgctcgtgag cacaacttaa aagatatcag tgtcgatata ccccgcgaca
     7141 aattcgtggt aatcaccggt ctgtctggct ccggtaaatc atcactggct tttgatacta
     7201 tctacgccga ggggcaacgc cgctacgtcg aaagtctgtc gagctacgcc cgccaattct
     7261 tgggtatcat ggataagcct gatgtcgatc agatagacgg tttgagtcca gctatctcga
     7321 tagaccagaa gtcggcttcc agaaatccac gttcgaccgt ggctacagta accgagatct
     7381 atgattacct acgtttgcta tttgcccgcg tgggcgtgcc gcactgtccg atcgatggtc
     7441 aagtcgtcaa gcgccagacg accgagacta tcatcaactc tgtcatggag ttgggcgacg
     7501 ataagcggtt gattattttg gcgccgattg tttctgaaaa aaagggtgaa tttgcccata
     7561 ttccagaaaa atatcaaaaa gccggctttg ctcgagtccg ggtagacggt gttatctacg
     7621 ctctggatga gtggccgcag ctcgataaga attataaaca caccatagaa ctagtggttg
     7681 accgtctagt actcaatcca tcggcgcgtg gtcgggtggc ccagtcggtc gaacaagccc
     7741 tggagattgc cgacagcgtg gtcgcagtac tcgatgctga cagcgatgaa ttacatatct
     7801 tttcgcagcg ctatgcctgc ccggaccatc ccgatttggc catacctgag cttgagccgc
     7861 ggactttttc gtttaactcg ccgttcgggg cttgtcctgt ttgtactggt ctcggcttcc
     7921 gtcaagaagt tgatccggaa ctagtgttta atccgaactt aactatcatg gagggtgcca
     7981 tccggcctta taaccggatt aattccgacg cttggtatac caaaaaactg aaggctctag
     8041 gcgaagcgca caatttctcg gtccacgtgc cagtcaaaca actaaccgcc gaagagcttg
     8101 ataagattct ttacggcacc ggtgctcaaa aatataccgt cgaactcggc aacggtcgtc
     8161 attttcagac aacttacgaa ggagttatcc ctaatctgga acggcgtcat cgtg
//
LOCUS       DASSBW010000015         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1296086, whole genome shotgun sequence.
ACCESSION   DASSBW010000015 DASSBW010000000
VERSION     DASSBW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296086"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFG56_00380"
     CDS             <1..605
                     /locus_tag="VFG56_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-specific 2-thiouridylase"
                     /protein_id="HET6622377.1"
                     /translation="TYFLYRVTGKALERTLFPLGDFKKSEVRRMAKERGLITAERRDS
                     QGICFVGQIGIKEFLSQYVETESGDIVDRSSGQMVGRHDGAIFYTLGQRHGLNVGGGL
                     PYYVVGKDMQKNEVYVSRDLNDQAMWRQELHLDHLHWINHPPKATDKLRVRLRHRGEL
                     KTAVLDGDRLKLDEPERAVAAGQSAVIYRGDEVLGGGIVS"
     gene            complement(646..885)
                     /locus_tag="VFG56_00385"
     CDS             complement(646..885)
                     /locus_tag="VFG56_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622378.1"
                     /translation="MYTQEKVAEERPTACLVIAVVFAVAALMLAAAALRAYVSRSETE
                     PDVWLVSFVPMAICGAFSLACGYLYFAKDEPQEMR"
     gene            967..>2554
                     /locus_tag="VFG56_00390"
     CDS             967..>2554
                     /locus_tag="VFG56_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET6622379.1"
                     /translation="MNAQQIRQKYLEFFEKRQHAVVRRAALVLTDDPTTLFTGSGMQP
                     MIPYLLGEPHPDGQRIVDSQTCLRAQDINDIGDNRHTTFFEMLGNWSMGDYFKEQQIE
                     WMFEFLSEVVGLDMSKVYITCFIGDKRYDIPKDTEAAAVWKKLYESNGLSSDEAEIGS
                     EEDGYRRGVREGERIFYYDGSKNWWSRNGDPEHTPVGDPCGPDSEMFYDFGTPHNPEY
                     GEHCHPNCDCGRFMEIGNNVFMAYKKVGDGQFEELEQKNVDHGSGLERIAAAALSDPD
                     VFKISLMWPIIEKLQDLSGKSYDSHTISMRVIADHLRAATFLAVDGCKPSNKEQGYVM
                     RRLIRRAIRYSFDLGIEQNFLEQIVPVIADLYHDDFPEVADNRGDIVAVLAKEEKAFR
                     QTLRKGLKEFEKFAEKGLTGDELFTLYDTYGFPVELSTEEAYRRDTQLPDDWREQFDA
                     KMEEQRNRSRTASKGEFKGGLGGQSDIHKKYHTATHLMYQALRDVLGHHVIQHGSNIT
                     EERLRFDFSHPDKMTPEQITM"
BASE COUNT          656 a          650 c          675 g          573 t
ORIGIN      
        1 aaacttactt cctataccgt gtaaccggta aggcactaga gaggacatta tttccactgg
       61 gtgatttcaa aaagtctgaa gtacgacgaa tggctaagga gcgcggcttg attacggccg
      121 agcgtcgtga ctctcagggt atctgcttcg ttggccaaat cggcataaaa gaattcttaa
      181 gccagtacgt cgagactgag tcgggagaca tcgtcgaccg atccagtggt cagatggtcg
      241 gtcgtcacga cggcgctatc ttctatacgc ttggtcaacg gcatggtttg aacgttggtg
      301 gtggtttgcc gtactacgtc gtcggcaaag atatgcagaa aaatgaagtc tatgtcagtc
      361 gcgacctcaa cgaccaggct atgtggcgac aggaactaca cctggaccat ctgcactgga
      421 ttaatcatcc gcctaaagcc actgataaat tgcgggttcg tttgcgtcat cgcggtgaat
      481 taaaaacagc cgtcttggac ggcgatcgat tgaagctcga tgagccggaa cgcgcagtcg
      541 ccgccggtca atcagccgtt atctaccgtg gcgacgaagt gcttggtggc ggcatagtct
      601 cataaaaatc tgtccctctt gcccgtgagg gcaagaggga cgatgtcacc tcatctcttg
      661 tggctcgtcc ttggcaaagt agaggtagcc gcaagctagg ctgaaagctc cacatatggc
      721 catcggcaca aagctgacca gccagacgtc tggctccgtt tccgagcgcg agacgtatgc
      781 acgtaacgct gccgcagcca gcatcaacgc tgcgacggcg aacactacag ctatcaccaa
      841 gcaggcggtc ggcctttctt cggcaacctt ctcctgtgta tacaccctta tcagctcctc
      901 tcaaagtgtg acaagcttac cataccacta aaattatcag tcgccaaaca tgctacaata
      961 ccagagatga atgcccagca aatccgccag aaatacttag aattttttga aaaacggcaa
     1021 catgccgtcg tccggcgggc tgccttggtt ttgaccgatg acccaaccac gttatttacc
     1081 ggttccggca tgcaaccgat gatcccgtat cttttaggtg aaccgcatcc agacggtcaa
     1141 cgcatcgtcg actcgcagac ttgcctgagg gctcaagata taaacgacat cggcgacaac
     1201 cgtcatacca cattttttga gatgctcggt aactggtcga tgggtgatta tttcaaagag
     1261 cagcagatcg agtggatgtt tgaattcttg tcggaagtcg tcggcttgga tatgtccaag
     1321 gtctacatca cctgttttat cggcgacaag cgctacgata tcccaaaaga caccgaggcg
     1381 gcagctgttt ggaagaaact ttacgaatcc aatggcttga gctcagatga ggctgagatc
     1441 ggctccgagg aggatggtta ccgacgcggt gtgcgggaag gtgagcgaat tttttactac
     1501 gacggttcga agaactggtg gagccgcaat ggcgatccgg agcacacacc tgtcggcgat
     1561 ccttgcggtc cggacagtga gatgttctat gacttcggca caccgcacaa ccctgagtac
     1621 ggggagcact gtcaccctaa ctgcgactgt ggccgcttca tggagatagg caacaacgtc
     1681 ttcatggcct acaagaaagt tggcgatgga cagtttgaag aactggagca gaaaaacgtc
     1741 gatcacggct ctggtttgga gcgcatagcc gccgctgctc taagtgatcc ggacgttttc
     1801 aagatcagcc tgatgtggcc aatcattgag aagctccaag atttaagcgg caagagctac
     1861 gattcacaca ctatcagcat gcgcgtcatc gccgaccact tgcgggctgc tacattcctg
     1921 gccgttgacg gctgcaaacc gtccaataaa gagcagggtt acgttatgcg acggctaatc
     1981 cgccgagcta tccgctatag cttcgacctt ggtatcgaac aaaacttctt ggaacagatc
     2041 gtaccggtta tcgctgatct atatcatgat gattttccgg aagtcgccga taatcgtggg
     2101 gacatcgtcg cagtactagc caaggaagaa aaagcctttc gacaaacgct gcgaaaaggt
     2161 ctgaaagagt ttgaaaagtt tgccgagaag ggcctaaccg gtgacgaatt atttactcta
     2221 tacgacacct atggttttcc ggtggagttg tcgacggaag aagcctatcg gcgtgatact
     2281 caattgcccg acgattggcg agagcagttt gacgccaaga tggaagagca gcgcaaccgt
     2341 tcccggaccg ctagcaaagg cgaatttaaa ggtggccttg gcggccagtc cgacatccac
     2401 aagaagtacc acacggcgac acacttgatg taccaagcac tacgcgatgt ccttggacac
     2461 catgtcattc agcacggcag caatatcaca gaagagcggc tgcggtttga tttcagtcac
     2521 cctgataaaa tgacgccgga gcagattact atgg
//
LOCUS       DASSBW010000016         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1198728, whole genome shotgun sequence.
ACCESSION   DASSBW010000016 DASSBW010000000
VERSION     DASSBW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198728"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..195)
                     /locus_tag="VFG56_00395"
     tRNA            complement(121..195)
                     /locus_tag="VFG56_00395"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Gln,seq:ctg)
     gene            complement(269..847)
                     /locus_tag="VFG56_00400"
     CDS             complement(269..847)
                     /locus_tag="VFG56_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622380.1"
                     /translation="MLKIKRPKPIRQISSGLMIVSVGFISSFLALSYMGYGLDNQTGD
                     NGHVLGSSTSQSSDDNGDSSDVTNADKSSSSDSSTPPKADKPKASSTATPTSWSGQAG
                     RGGGGSGGSSSSTSIDPAGYTVTVSPSPTGGSSTGSLPISPSHSSDGGGTSSSSGGSL
                     TDPVTDTLKTTTDKADKTLEAAKDTTGSLLGG"
     gene            1014..1487
                     /locus_tag="VFG56_00405"
     CDS             1014..1487
                     /locus_tag="VFG56_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622381.1"
                     /translation="MKQKKNTDPSTEHAGYTHLRQKLSSVLLVQMVLNVAGYGYHTMV
                     DHLPMWLLWLLIINTIAMFGLALVIAPRQMIITFGSIIGSLLITAAIGAAYLWVIASF
                     MPGVSTAIENDNSQVLTITALTGLILLSISFGMCLHYTGKALSRFLRPKTDGTAR"
     gene            1471..2760
                     /locus_tag="VFG56_00410"
     CDS             1471..2760
                     /locus_tag="VFG56_00410"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET6622382.1"
                     /translation="MAQHDNQTVKLTSIVGGGQALGVLESGKKVFVWGGLPDETVEVR
                     ITKKKSSYAEAVVERVIKASPQRVQAKDAAFLSTSPWQIMDFEAEQHYKSALIEEAFE
                     LHDIVLPGPIKVFSDNQIYHYRNKVEFSFWWDNDKNILDLAFFRRGTKGKLPVEDTSL
                     ARPEINALATAIRDILRSKQVEARDLKTLMIRSSQSGQVAFQLYVKDKKCQPLEATDF
                     ESLPAQGGEVIYSDPKSPASKITEVLTSFGQTKLSDNLLEHDFSYVVNGFFQVNLPVY
                     EQALQDMKNYLSNRPLIDMYAGVGSIGLSIGPSRTTLVELDPFAVSEMKRNIEANGMQ
                     ATAVLAASEKALDHIASDRDIIVDPPRAGLHKDVINKLLAAKPPRIIYLSCNPVTQAR
                     DVALLAEKYGIKAHRGYNFFPRTPHIEHLVVLDLKGL"
     gene            complement(2747..3235)
                     /locus_tag="VFG56_00415"
     CDS             complement(2747..3235)
                     /locus_tag="VFG56_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622383.1"
                     /translation="MNDAIYDDLAIEGSIREQFSLQLDIKHVIVRGIPTSRTTKASVF
                     LTTKNKLFVYIDGQEPLSLSDVTKMIKRMGLVAKAYLPPRREDDYFDRIARGHFKKVY
                     PGHPNPSDEDLRYYRSLAPYNPALVEVEEITAGVIYQFDSNDSSNWRTAAKFSYRRIK
                     AL"
     gene            3308..3682
                     /locus_tag="VFG56_00420"
     CDS             3308..3682
                     /locus_tag="VFG56_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6622384.1"
                     /translation="MDQTKTILIIDDDKTLLDALASALSEAGYKTLSAGSGQEGLELA
                     KQAQPDLTLLDYQMPEMNGIETLEQLRRDDWGRKAKVVITTNIYDINIVNQALANGVS
                     DYLLKADTPLDEIVRLVDKYVK"
     gene            3695..4984
                     /locus_tag="VFG56_00425"
     CDS             3695..4984
                     /locus_tag="VFG56_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6622385.1"
                     /translation="MGKPKTGVSQLFKPHSDGILVATSLKITLIMVTALTVGFSASFI
                     VNDMYRSYLSSRVASVAAAWPAEVSDQAAQRRLNRIKTTYDGASQVYLVKPTDSGGYN
                     RIDADGQTKQPASIYLISQLYNLTGPAIKGPLSNHWQKHVITTSAVVDPITGQAVALI
                     GIDVLSSTYVLVVALAIIIPLIAGLMISLIVVAADRIRLRRREALRFRSELVSIASHE
                     LRTPLTGLRWSEEGLLKASLAKTYHQTIKTMYDSTLRLQESIEDILQLANLQAGRNLG
                     LNKTSTDLTELLNSIMTIQKLPAEQKNLTLTFADDWPKRLMINCDAQRMKRVFNNLVS
                     NAIKYSQPGTEILLDHEKLGDKHLISLIDQGIGIPEMEQSKVFSGFYRATNAVKHQSN
                     GTGMGLYLSRQVIEQHDGKLWLKSKVNQGTTVYIELP"
     gene            complement(4986..>5227)
                     /locus_tag="VFG56_00430"
     CDS             complement(4986..>5227)
                     /locus_tag="VFG56_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622386.1"
                     /translation="TSMISGRSQLKSHSQLMTIIQMDGQGSQPAKLGTWRSLRADAPK
                     GVYFGWKNFYDEDKPTLSPKRTMALKPQPWYVSYQ"
BASE COUNT         1387 a         1361 c         1267 g         1212 t
ORIGIN      
        1 gatggaggga ttcgctttga ccgatcaatc agccaaagtg ccggggagca aagacttttg
       61 ctcccgttcg aatccgctcc gtagtcacgc aaaaaccgga cataaagtcc ggctctagcg
      121 tggctgggat ggagggattc gaacccccga atgctgggac cagaacccag tgccttacca
      181 cttggcgaca tcccatcggc ttcgcagtct taaattatac cgatggagcg ctcaagcggc
      241 aagttataaa cgactagtaa atgatgttct atccacccag taaggatccg gttgtgtctt
      301 tggctgcctc taatgtcttg tcggctttgt cggtagtcgt cttcagtgta tcagttaccg
      361 gatcggtcaa gctaccgcca ctagaagagg atgtgccacc gccatcacta gagtggctcg
      421 ggctaatcgg caaggagcct gtcgagctac cgccagtcgg cgaaggcgag acggtcaccg
      481 tgtagccagc aggatcgata ctagtggaag agctcgatcc acccgaacca ccgccaccac
      541 gtccagcctg acccgaccag ctggttggcg tagcggttga gctggcctta ggcttgtcag
      601 cttttggcgg agtagacgaa tcggaagagg atgatttatc ggcattggtc acatcactgc
      661 tatcaccatt gtcgtcgcta gactggctag tgctagagcc taagacgtgg ccgttgtcgc
      721 cggtttgatt gtcgagacca tagcccatgt agctaagagc taaaaaactt gagatgaagc
      781 cgacactaac tatcatcaga ccggagctga tttgccggat tggcttggga cgcttaattt
      841 taagcataga aactcctttc gtttcctagt ccaatcctag gcgacagttt gggtctgtgc
      901 agtgacaatt attacaatcg tagacttttt cgcaacaagc gtaagcgtaa tggtcagtag
      961 aatagctcag ccaacatacc aggcggctca tgataagcta taggtaacat ccgatgaagc
     1021 aaaagaagaa tactgatcca tctactgaac atgccggcta cactcatctc cggcaaaagc
     1081 ttagctctgt tctgttagtt caaatggtcc taaacgtcgc tggttacggt taccatacca
     1141 tggtcgatca tctgcccatg tggctgcttt ggttattgat catcaacacc atcgccatgt
     1201 ttggcttagc cttagtgatc gcaccgcgtc agatgatcat aactttcggc tccatcatcg
     1261 gctcgttgtt gatcacagcc gctattggcg cagcttacct atgggtaatc gccagtttta
     1321 tgcctggagt ttcgacagcc atcgagaacg acaactcaca agtgctcacc atcacagcgc
     1381 tcaccggcct gatcttactg agcatatcct tcggcatgtg tctgcactat acaggaaaag
     1441 ccctgagtcg ctttttaaga cccaagactg atggcacagc acgataatca gaccgtaaaa
     1501 ctgaccagca tagtaggcgg aggtcaggcg ctcggcgtac tcgaatcagg caagaaggtg
     1561 tttgtatggg gcgggttacc agacgagaca gtcgaagtcc gcataactaa gaaaaaatca
     1621 tcctacgccg aagctgtggt tgaacgggtg ataaaggctt cgcctcagcg tgttcaggca
     1681 aaagacgccg ccttcttatc gactagtcca tggcagatta tggacttcga ggcagagcaa
     1741 cattataaat cagctcttat cgaagaggca tttgagctac acgacatcgt attgcccggg
     1801 ccgatcaagg ttttttcaga caaccagatt tatcactatc gcaacaaagt cgagttttct
     1861 ttctggtggg acaacgataa aaatatcttg gatctggctt tcttccgccg aggaacaaaa
     1921 ggtaagctgc cggtcgaaga tactagctta gctcgaccgg aaatcaacgc tctagctacg
     1981 gccatacgag atatcttgcg gagcaagcaa gtcgaagcgc gtgatctaaa gaccttgatg
     2041 attcggtcta gccaatccgg ccaggtggct tttcaactct acgtcaaaga caagaagtgc
     2101 caaccactag aagctacaga ttttgagagc ctaccggccc aaggcggaga agttatctac
     2161 tccgatccga aaagtccggc ttctaagatc accgaagtac tgaccagctt cggtcagaca
     2221 aaactatccg acaatctgct ggagcatgat ttcagttacg tcgtcaacgg cttcttccaa
     2281 gtcaacctgc cggtttatga acaagctctt caagatatga aaaactacct atcaaaccgg
     2341 ccattgatag atatgtacgc cggtgttggt agtatcggtc tatcgatcgg accgagccga
     2401 accaccttag tagaactcga cccgttcgcc gtctcagaga tgaagcgtaa tatcgaagcc
     2461 aacggcatgc aggccactgc cgttttggct gccagcgaaa aagctttgga tcatatagcc
     2521 tccgaccgag acatcatagt cgacccgcct cgggcgggac tacacaaaga cgtgatcaat
     2581 aaattactag ccgcgaaacc accgcgaata atttatctga gctgcaatcc agtcacgcaa
     2641 gctcgtgacg tggcgttact ggccgagaaa tacggcatca aagcccaccg cggctacaac
     2701 ttttttccgc gcactccgca catcgaacac ttagtcgtac tcgaccttaa agggctttaa
     2761 ttcgtcggta agagaatttg gctgccgttc gccagttgga cgagtcgtta gaatcaaact
     2821 ggtagatgac accggccgtg atttcttcga cctcaaccaa ggctgggtta tacggagcga
     2881 ggctacggta gtaccgtaga tcttcgtcgc tagggttagg atggccggga tatacctttt
     2941 taaaatgccc acgggcaatc cgatcgaaat aatcgtcctc tcgccgcgga ggcagataag
     3001 ctttggcgac taagcccatc cgcttgatca tcttggtaac atcactaaga ctcagtggtt
     3061 cctgcccgtc aatatagaca aaaagcttgt ttttcgtcgt caagaagacg cttgccttgg
     3121 tggtcctaga ggtcggaata ccccggacta tgacgtgctt gatgtcgagc tgcaagctga
     3181 attgttcacg aatcgaacct tcgatcgcta gatcatcata gatagcgtca ttcataccga
     3241 tattgtagca ccattgcgcc tctgggccgc tttgcggtaa gatgaaataa attaacaaag
     3301 ggtagacatg gatcaaacca aaaccattct aataattgat gatgacaaaa cactgcttga
     3361 cgctctggcg tcagccttgt ccgaagccgg ctacaaaacg ctaagcgccg gtagcggtca
     3421 ggagggcttg gagctagcca aacaggctca gcccgatctg acactacttg attatcaaat
     3481 gcccgagatg aacggtatcg aaacactcga acaactccgg cgagatgatt ggggtaggaa
     3541 agccaaggta gtcatcacta ccaatatcta tgacatcaat atcgtcaatc aagctctagc
     3601 caacggcgtt agtgactatc tattaaaagc cgacacgccg ctcgacgaaa tcgtccgact
     3661 agtcgacaag tatgtaaagt agcccggagg cagggtgggc aaaccaaaga ccggcgtcag
     3721 tcagttgttc aaaccgcata gcgacggcat actggttgct acttctctaa agatcaccct
     3781 aatcatggtg acagccctga cagtgggctt cagcgccagc tttatcgtca acgacatgta
     3841 ccgctcgtat ttaagcagtc gagtagctag cgtggccgca gcttggccgg ccgaagtcag
     3901 cgatcaagcc gcacagcggc ggctcaaccg tatcaagacg acttatgacg gcgctagcca
     3961 agtctacttg gtcaagccga ccgacagcgg tggctataat cgaatcgacg ccgacggtca
     4021 gactaaacaa ccagcttcaa tttatctgat cagccagtta tacaatctaa ccggtccagc
     4081 catcaaaggt ccactgtcca accactggca aaaacacgtt atcacaacct ccgccgtggt
     4141 tgacccgatc accggccaag ccgttgccct tataggtata gatgtcctgt cgtcaaccta
     4201 cgtattagta gtggccctag ctatcatcat ccccttgatc gctggtttga tgattagtct
     4261 aatcgtcgtc gcggccgacc gtatccgcct acggcgtcgg gaagcgctac gttttcgcag
     4321 cgaactagtc tcgattgctt cacacgagct gagaacgccg ctcaccggcc tacgctggag
     4381 cgaggagggg ctactcaaag ccagccttgc caaaacttac catcagacga tcaagacgat
     4441 gtatgactcg accttacgtt tgcaagagag tatcgaagac atactgcaac tggccaatct
     4501 ccaagccgga cgcaatcttg gcctaaataa gacctcgacc gatctaactg aactattgaa
     4561 cagtattatg accatacaaa aattaccggc cgagcaaaag aatttgacac tcacatttgc
     4621 cgatgactgg ccaaagcgcc tgatgatcaa ctgtgacgct caacgaatga agcgtgtctt
     4681 caataacctg gtgtcaaacg ccatcaagta ctctcaacca ggcaccgaaa tactactcga
     4741 ccatgagaag cttggtgaca agcatcttat cagcctgatt gatcagggta ttggtattcc
     4801 ggaaatggaa cagtccaagg tcttcagtgg tttttaccga gcgactaacg ccgtcaaaca
     4861 ccagtccaac ggtaccggta tggggttata tttgtcgcgt caggtgatcg agcaacatga
     4921 cggcaagcta tggctcaagt ccaaggtcaa ccaaggcact actgtctaca tagagttgcc
     4981 gtaagctact ggtaggacac gtaccatggt tgcggtttta aggccatggt acgtttcgga
     5041 ctaagagttg gcttatcctc gtcataaaaa tttttccagc caaaataaac tcctttaggc
     5101 gcatcggcac gcaagctacg ccaagtaccg agcttggccg gctgagagcc ttggccgtcc
     5161 atctggataa tcgtcatcaa ttgactgtga gactttagct gactacgtcc gctaatcatc
     5221 gatgtcc
//
LOCUS       DASSBW010000017         6598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_639906, whole genome shotgun sequence.
ACCESSION   DASSBW010000017 DASSBW010000000
VERSION     DASSBW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639906"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFG56_00435"
     CDS             <1..420
                     /locus_tag="VFG56_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6622387.1"
                     /translation="GFTLKALCGMLINLAVGIFDKAQIGNQAAQEAGGEKLEKVSNNV
                     AGPIGILGIIFPAAREAGLTTLVMLTAIISLTLAVMNVLPIPGLDGGRWFLMALFKVL
                     KKPLTKEREEKIVATGMMVLFGLIILVTIADIGKLGG"
     gene            421..1167
                     /locus_tag="VFG56_00440"
     CDS             421..1167
                     /locus_tag="VFG56_00440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6622388.1"
                     /translation="MNQIKQTAISFVYGLGQSLWVIFGFGVAAYLAVLALHDLIVANP
                     TRLDLIFSANALGYFLAVIIVVGVAAMITRESWSATKRLLALNPAKWTKYLKVLAVFG
                     AYIIVSQIIYFVVDIVLSDIINLNQKQNLGFTADSLVQPYQYVLAFFMLVILPPVFEE
                     ILFRGYLFGRLMQKMGFIASAIVTSFVFGLVHFNWSVSIDVFVLSLFLCYLRRDSDSI
                     VPGMLLHGLKNGLAYFLLFIAPLLGIHLVK"
     gene            1174..2409
                     /locus_tag="VFG56_00445"
     CDS             1174..2409
                     /locus_tag="VFG56_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HET6622389.1"
                     /translation="MRRSQLFTKTLRDAPADEQAKNAQLLIRAGYVYKEMAGAYAYLP
                     LGLRVVEKIKQIVREEMNAIGSNELIMTGLQNKAVWEKTGRWDDEVVDIWFKTSLQDG
                     TELGLGWSHEEPIVEMLKNFVSSYKDLPISLYQFQTKMRNELRAKSGIMRGREFVMKD
                     MYSFHSSVKDLGKYYDQTIEAYKRVYDRLGIGRDTYVTFASGGAFTKFSHEFQTICEA
                     GEDVIYLHRGKNIAVNEEVLDDAVSELGVSKDELEPVKTAEVGNIFNFGSQKADEMGL
                     KFTDHSGKQGSIYMGSYGIGVTRVMGVLAEKLSDDRGLVWPEEVAPFKVYLASIGGAE
                     AEADKLYDKLSKSGVEVLYDDREVRPGQKFADAELLGIPHRLVVSPRLTEQGKIEYQN
                     RQETQSEILTEADIIARLI"
     gene            2497..2952
                     /gene="greA"
                     /locus_tag="VFG56_00450"
     CDS             2497..2952
                     /gene="greA"
                     /locus_tag="VFG56_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET6622390.1"
                     /translation="MNKTYHLTVEGKKELEAELEELKPQRAIIAEKIANARDYGDLSE
                     NAEYDAAREEQGLLESRIAEVEEILKNADIIEAGKSDGTVKVGSTVDLHNGKDVTYQI
                     VGPVEADPLEGKVSNESPIGSALLGKKAGDKVTIKTPKGEMGYTVKKVY"
     gene            3011..4180
                     /locus_tag="VFG56_00455"
     CDS             3011..4180
                     /locus_tag="VFG56_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622391.1"
                     /translation="MVLSDKQLETLARENGTPLFVYDQAYLQDRADLLVGLEAKLPFG
                     FTPRFAAKANMYPGIIELFDEAGLGFDAGSSHEAMELIQLGVDGQKISLSSQQPADNL
                     AELLESKVNYIATSLHQLELFAEAAGRGAKVGLRVNPGLGAGHNNRTTTGGANSSFGL
                     WNAYLSQALQLAKDKGLTINKLHVHIGSGADPLVWRATIETALELVEQMPDVAVLNLG
                     GGYKIHRFGDEREADMDEIIDVFAEELRAFADQTDRQLHLEIEPGTWLTGHSGVLLSQ
                     IQDIVDTGPDGHYFLRLDTGMNDFIRPSMYGAQHDIKVLNGSKETRDYVVVGHNCETG
                     DILTPAPGDPEGIEPRQLNEAAIGDLIAIYDCGAYCASFSAHGYNSYPDAKQILV"
     gene            complement(4213..4389)
                     /locus_tag="VFG56_00460"
     CDS             complement(4213..4389)
                     /locus_tag="VFG56_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622392.1"
                     /translation="MNRLPMYTIVGLGLTLGNLLPTLFGQSPFGLWGILGTLIGGMVG
                     VWVFWKLRQMGYIE"
     gene            complement(4391..6337)
                     /locus_tag="VFG56_00465"
     CDS             complement(4391..6337)
                     /locus_tag="VFG56_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6622393.1"
                     /translation="MKQLIKLSLLAGVLTVLWLPQQTSASVYGGGEFGNCDYQQDCPP
                     PPADDADETPTTDQPVPADPPKTPTSQSKTSVNLNVTEQSVITNTPFTITANVTTIVG
                     EDGQPVRADNVGWVAFYVDDRLVSTDYSPDANGNYSADWYIDGDSDRIVTAVAYDSAG
                     QVLGRQDTPVTVRLIEQPSDTDQDSGTGSQSDGKSGDGGGWIAKLLGENDGIPGNGVG
                     AVGHLIHHSVPPAVAKSFPYWIFVILLLLVLRLVWQTVRETIANNHMLALLNKQRLIA
                     EEKDNFIALSSHYLHTPLTVMSNGADTMVAVHESTPETLSALTAALGNLKQKIDKILD
                     DVENNTALKNIQAPTFSPAQLKGFSSPFFWLPLVAVAGICLLANFLLGVIGQIDLGIN
                     NLWVQVVVFLVVAILFYTFFRTHQLRRRETDKSRQLIDYERTIDTARNSFIQDSTAAL
                     RQGLNEVEEAKKPLADSPTKRFVDDGHQRFAEILAKFELLSQIETTSMEADKQDFTLS
                     ESVGNVLANYQEAIADKNLTVETNFHNIAVHENRRLFEYVLGTIIDNAVKFSAVNGHV
                     LISADKTSGRLHLKVQDNGQGIPVDKLPHLFKPFSRADSAIQFDYEGLGFSLFLDRII
                     LDHLGGDIKADSQPMQGTLITVTA"
BASE COUNT         1645 a         1523 c         1786 g         1644 t
ORIGIN      
        1 ggttttacgt tgaaggcgct ctgcgggatg ctgatcaact tggctgtggg tatatttgac
       61 aaggctcaga ttggcaatca agccgctcaa gaggctggtg gtgagaagct tgagaaagtt
      121 tcaaataacg tagctggccc aattggtatc ctcggtataa tcttcccggc ggcgcgtgaa
      181 gcggggctga cgacactcgt catgctgacg gctattattt ctctgacctt ggcggttatg
      241 aatgtcctgc ctatcccagg cctggatgga ggccgctggt tcctgatggc gctatttaag
      301 gttctcaaaa aaccactaac caaggagaga gaagagaaaa tcgtggccac tggcatgatg
      361 gtgctgttcg ggttgatcat tcttgtaacc attgctgata tcggtaaact aggcggctag
      421 atgaaccaga taaagcaaac agccatttcg tttgtatatg gcttaggcca gagcttgtgg
      481 gtgattttcg gtttcggcgt agccgcctac ttggccgtgc tagcccttca tgatctgatt
      541 gtcgccaacc cgactcgact tgatttaata tttagcgcca atgcgcttgg ttatttcctg
      601 gcggtgatta tcgtagtcgg cgtagcggct atgattaccc gcgagagttg gtcggcgact
      661 aaacgattac tggctttgaa tccagctaag tggacgaagt acctaaaagt tctagcggtt
      721 tttggtgcct atatcattgt ttcacagatc atctatttcg tcgtggatat cgtattgtct
      781 gacatcatca atcttaatca gaagcagaat ttaggcttta cggccgacag cctggtccaa
      841 ccgtatcaat acgttctagc ctttttcatg ctggtaatac tgccaccggt ctttgaggaa
      901 attctttttc gtggctatct gttcggtcgc ctgatgcaaa agatgggctt catcgccagc
      961 gccatagtta caagtttcgt cttcggcttg gtccacttca actggagtgt gtcgatcgac
     1021 gtattcgtcc tcagcttgtt tctttgctat ttgcgccgcg attccgattc tatcgtaccg
     1081 ggtatgctgt tgcatggtct gaaaaacggc ctagcgtatt ttttactgtt tatagcaccg
     1141 ctcctcggta ttcatctggt aaaatagttt ggaatgagac gttctcaact ttttaccaag
     1201 acgcttcgcg acgctccggc tgacgaacag gccaagaacg cccagctgct catccgagcc
     1261 ggctatgttt ataaggaaat ggctggagcc tacgcctact tgccactcgg ccttcgcgta
     1321 gtcgagaaaa tcaagcaaat cgttcgtgaa gaaatgaacg ccataggttc caatgagctg
     1381 atcatgacag gacttcagaa taaggcggta tgggagaaga ctggtcgttg ggatgatgaa
     1441 gtagtcgaca tatggttcaa aaccagtctg caagacggta cggagctagg tctgggctgg
     1501 agtcacgaag agccaatcgt cgagatgctg aagaactttg tctcgagcta taaggatttg
     1561 ccaatcagcc tgtaccagtt ccagacaaag atgcgtaatg agctgcgagc taagtcgggt
     1621 atcatgcgtg gtcgcgagtt cgtcatgaaa gacatgtact cctttcactc tagcgtgaaa
     1681 gaccttggta agtactatga tcaaaccatc gaagcctata agcgggtata cgatcgtttg
     1741 ggtatcggca gagatactta tgtgacgttc gcctctggcg gtgcgttcac taagttcagt
     1801 cacgaatttc agaccatttg cgaggctggt gaagacgtca tctacctaca ccgcggtaag
     1861 aatatcgctg taaacgaaga agtcctagat gacgccgtat ctgagttggg tgtcagcaaa
     1921 gatgaattag aaccagttaa aacggctgaa gtcggtaaca tcttcaactt tggctcgcaa
     1981 aaagccgatg agatgggtct taagttcacc gaccatagcg gcaagcaggg ctctatctat
     2041 atgggctcgt acggcatagg tgttactcga gtcatgggtg tactggccga gaagctgtca
     2101 gacgaccgtg gcttggtttg gccggaagag gtagcgccgt ttaaggttta tctagcttcg
     2161 atcggtgggg ccgaagccga ggccgataag ttgtacgaca aattaagcaa gtctggcgta
     2221 gaggtactct atgacgaccg ggaagtgcga ccgggtcaaa aatttgccga cgccgaattg
     2281 ctcggcatac cgcatcgctt agtcgttagc ccacggctta ccgaacaggg gaaaatagag
     2341 tatcagaatc gtcaagagac gcagtcggaa atattgactg aagccgacat aattgctaga
     2401 ctaatctaat tagaaattgg gggcaggtca acgcaaaaat caaacagtgc gacgacctaa
     2461 tttttaatta aattagcatt agaagaggtt aacaatatga ataaaactta tcatctgacc
     2521 gtggagggta aaaaagagtt ggaagctgag ctagaagagc tcaagcccca gcgggctatc
     2581 atcgctgaga agattgctaa cgctcgcgat tacggtgact taagcgaaaa cgccgagtac
     2641 gacgctgctc gagaagagca gggactactt gagagccgga tcgctgaagt cgaggaaatt
     2701 ctaaaaaatg ccgatattat cgaggccggt aaatcggacg gtacggttaa ggttggctcg
     2761 accgtcgatc tacataacgg taaggatgtc acttaccaga tcgttggtcc agttgaggct
     2821 gatccgctag aaggtaaggt atcgaacgaa tcgccaatcg gctcggccct gcttggcaaa
     2881 aaggccggcg acaaggtcac catcaagaca ccaaaaggcg agatgggcta caccgttaag
     2941 aaagtctact agaactagcc tctacgctat aacaccggga caccgaatcc cggtgttata
     3001 atttagggca atggtattat ccgataaaca gctcgagaca ctggctcgcg agaacggcac
     3061 gccgttgttc gtttacgacc aagcctacct tcaagaccgg gcagatttgc tggttggtct
     3121 ggaggctaag ctgccgtttg gtttcacgcc tcggtttgcc gccaaggcca acatgtatcc
     3181 cggtatcatc gagctgtttg acgaagctgg gctaggtttt gatgccggct ccagtcatga
     3241 ggctatggag ttgatccagc ttggtgtcga cgggcagaag atcagtttgt cgagtcagca
     3301 accggccgat aatctagcgg agctactcga gtccaaagtt aactacatcg ccacatcact
     3361 ccaccagcta gagttgtttg ccgaagcggc cggtcgaggc gccaaggttg gtttaagggt
     3421 caatcccggt ctgggggctg gtcacaataa tcgaacgacg accggtggtg ccaactccag
     3481 ttttggtcta tggaatgcct atctatccca agcattacag ctggcaaaag ataaaggcct
     3541 gaccatcaat aagctgcatg tccacattgg ctcaggcgcc gatccgttag tctggcgggc
     3601 aacgattgag acagcactag agctagtcga gcaaatgccg gatgtcgcag tcttaaacct
     3661 cggcggtggc tacaagatcc accgattcgg tgatgaacga gaggctgata tggatgaaat
     3721 tatcgatgtc ttcgccgagg agttgagggc tttcgccgat caaaccgatc gccaactaca
     3781 tctagagatt gagcctggca cctggctaac cggtcatagt ggtgtattgc tgagtcagat
     3841 ccaggatatt gtcgacaccg gtccggacgg tcattatttc ttgagacttg ataccggtat
     3901 gaatgacttt atcagaccga gtatgtacgg cgcacagcac gatattaaag ttctaaatgg
     3961 cagcaaagag acccgggatt acgtcgtcgt cggtcataac tgtgaaactg gcgatatctt
     4021 aacgccagca ccaggcgatc cggaaggtat agagccacgc cagttaaacg aagcggctat
     4081 aggtgatttg atcgcaatct atgattgcgg tgcttactgt gccagcttct cagctcacgg
     4141 ttacaattct tatcctgatg ctaaacagat attagtctag aaatcttcat ccggcttggt
     4201 cttgatgatt gattactcaa tataacccat ctgacgaagc ttccagaaca cccagacgcc
     4261 aaccatacca ccaatgaggg tgcctaagat accccacaga ccgaatgggc tttggccaaa
     4321 gagagtaggt agtaagttgc ccagagtcag ccccaggcct acgatggtat acatcggtag
     4381 ccggttcata ttaagccgtt acggttatta gagtgccctg cataggctgt gaatcagctt
     4441 tgatatcacc acctaagtga tcgaggatga tacggtctaa gaacaggctg aagcccagtc
     4501 cctcatagtc gaactgtatg gctgagtcgg ctcgtgagaa aggcttaaag aggtgcggca
     4561 atttgtcgac aggtatacct tgtccgttgt cttggacttt gaggtgtaaa cggcccgagg
     4621 tcttgtcggc tgagattaag acgtggccgt tgacggctga aaatttgacg gcattgtcta
     4681 tgatggtgcc gagcacatac tcgaatagcc ggcggttctc gtgaacggca atgttgtgga
     4741 agttcgtctc gaccgtcagg ttcttgtcgg cgatagcctc ctggtaatta gctaagacgt
     4801 tacctaccga ttcgctaagc gtgaagtcct gcttgtcggc ttccatggaa gtagtctcga
     4861 tttgcgacag gagctcgaac ttagctagga tttcagcaaa gcgttggtgg ccgtcgtcga
     4921 cgaagcgttt agtcggtgaa tcagccagag gcttcttggc ctcttcaacc tcgttgaggc
     4981 cctggcgcag tgcggcggta gagtcttgga taaaactatt tcgggcggtg tcgatagttc
     5041 gttcataatc gatcagctga cggctcttat cggtttcacg ccgtcttagc tggtgggtcc
     5101 taaagaaggt atagaacaag attgctacca ccaagaagac aacgacctgg acccacaggt
     5161 tattgatacc aaggtcaatc tgaccgataa cacctagcag gaagttggct agcagacaga
     5221 tgccggctac cgccaccaat ggtaaccaga aaaagggcga actgaagccc tttagctgag
     5281 ccggtgagaa agtgggcgct tggatgtttt taagagcagt attgttctcg acgtcgtcta
     5341 ggatcttgtc gatcttttgt ttcagattac cgagagctgc agtcagagcc gatagtgtct
     5401 ctggtgtaga ttcgtggaca gccaccatgg tgtcggcacc gttgctcata acggttaatg
     5461 gcgtgtgcag atagtgcgaa ctcagggcga tgaagttgtc cttttcctcg gcgatcaggc
     5521 gctgtttatt gaggagggcc agcatgtggt tgttggcgat ggtttcacga acggtctgcc
     5581 aaaccaacct cagtactagc agaagcagga ttacgaagat ccaataggga aaggatttgg
     5641 ctactgccgg tggtactgaa tgatgaatca aatggccgac cgcaccgaca ccgttaccgg
     5701 gaataccgtc gttttcaccg agcaatttag cgatccagcc accgccgtca cctgatttgc
     5761 cgtcactttg gctgccagtg ccggagtctt gatcggtatc actaggttgt tcgattaacc
     5821 gaacggtgac gggcgtatcc tgccgaccga gtacttggcc ggcactgtcg taggctacgg
     5881 ccgtgacgat gcgatcggaa tcgccgtcga tataccagtc agcgctgtag ttaccgttag
     5941 cgtcggggct atagtcagtc gagactaacc ggtcgtcgac atagaaagcc acccaaccaa
     6001 cgttgtcggc ccgtaccggc tgaccgtctt cgcccacgat agtagtgaca ttggcagtaa
     6061 tggtaaacgg tgtgttagtg atgacgcttt gctcggtaac gtttaagtta acgctagtct
     6121 tagattggct agtcggagtc ttgggcggat cggctggtac cggctgatcg gtcgtcggcg
     6181 tctcatctgc gtcgtcagct ggcggtggcg ggcagtcttg ctgataatca caattaccaa
     6241 attcacctcc gccgtagacc gaagctgagg tctgttgcgg cagccacagt acggtcaata
     6301 ctccggcgag aagcgatagt ttgatcagct gtttcatttt agattctacc ttggttaaat
     6361 tttagcgctt atcactagtg accacaagcg ttttattggt ctatactagt agtagtaaac
     6421 aaaggagggc ttatgggcgg agcatacgag ttcaatggcg aactttataa caatccagaa
     6481 gagttcttag acgcagtcgc gcacgaatac aagtcaggtg acaaggacct ggccttacag
     6541 acactcaacg actatggttt tgagctaacc gacatcaacg ttcgaccgga cgagctga
//
LOCUS       DASSBW010000018         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_664085, whole genome shotgun sequence.
ACCESSION   DASSBW010000018 DASSBW010000000
VERSION     DASSBW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664085"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFG56_00470"
     CDS             complement(<1..592)
                     /locus_tag="VFG56_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622394.1"
                     /translation="MYNINLFATGPAILAFIATVFAAGCFQLSVSVLTLISSHTIGAS
                     RSFNKLLSLIASYLFGVLASIFVLAVLATQLVDSSRFFLGESVWLGLGVVSIAVGGLI
                     IIFYYRQSKGTSLWVPRSMAKFLNNRTKRTKSAVETFSLGIITTVAELPFSFVTFLVL
                     AYLAKNANTNTQLSLSLIYALAVSLPLIFVAATITSG"
     gene            751..915
                     /locus_tag="VFG56_00475"
     CDS             751..915
                     /locus_tag="VFG56_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622395.1"
                     /translation="MEAILDKVVGSSTWHRALDSHDLQNFVARHEQVLDGVDDEELSD
                     SAFDLGGLVE"
     gene            1054..1130
                     /locus_tag="VFG56_00480"
     tRNA            1054..1130
                     /locus_tag="VFG56_00480"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1088..1090,aa:Met,seq:cat)
     gene            1367..2212
                     /gene="tilS"
                     /locus_tag="VFG56_00485"
     CDS             1367..2212
                     /gene="tilS"
                     /locus_tag="VFG56_00485"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET6622396.1"
                     /translation="MSRKPMLVVAVSGGVDSMVMLDKLIKQSDHNLVVAHVNHGIRSD
                     SGEDEALVRQVAESHHLPVEVCRLHLGEGASEQVARQGRHEFLNKVKTDYGAEAVATA
                     HHLDDVAETIILNFMRGTGWRGLCSLRETNSLKRPLLDLRKSQVLSYAIQNGLHWHED
                     STNDDLRYTRNLIRHFYLSKLQPDQFDGLIALWRRQCILRPQIDELVSSLVESNFDRC
                     WLELDDAMTVELLRAWLGSNYQAATFKRLVKFGREALPGKKFNLPGDEMILAERDKLV
                     AIGRR"
     gene            2271..>3162
                     /locus_tag="VFG56_00490"
     CDS             2271..>3162
                     /locus_tag="VFG56_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET6622397.1"
                     /translation="MPIKTPRRPKRPSSNGFRTVVFWAIIIFVALGIIGYLTSGPNLE
                     KVPFSQVINEANSGKINKITIESNKLLVTTKAQDDQSKPTQVSYKEDGSSIYEQGLSQ
                     DAPVEVAVTPPSDTSNAVWNLATIIVPVLIIGGLFYFMFRQAQGQSNQAMGFGKSKAK
                     LYGSDKKKVVFEDVAGNNEAKEDLVEVVDFLKHPKKFRSVGAEIPKGVLLVGPPGTGK
                     TLLARAVAGQADVPFFSISGSEFVEMFVGVGASRVRDLFEKAKKNAPCIIFIDEIDAV
                     GRRRGSGMGGGHDEREQTLNQ"
BASE COUNT          854 a          731 c          803 g          774 t
ORIGIN      
        1 gtccgctggt gatggtcgcg gctacaaaga tgagcggcaa gctaacggcc aaggcataga
       61 ttaggctcaa agagagttga gtgttggtgt ttgcattttt cgctagatag gctagaacca
      121 ggaaagttac gaaactaaac ggtaattcgg ccacggtagt gatgatgcct agtgaaaagg
      181 tctcaaccgc tgacttggtt cgttttgtcc ggttgttgag aaattttgcc atgctacgcg
      241 gtacccacag gctagtgccc ttactttggc ggtaatagaa aataatgatc aaaccaccta
      301 ctgcaatact gacaacccct aggcctagcc atactgactc gcccaagaaa aaccggctac
      361 tgtcgactag ctgggtggct agtactgcta agacgaaaat cgaagccaag acaccgaaga
      421 gataactggc gattagactg agtagtttat tgaaggatct ggaggctccg atagtgtgag
      481 agctgatgag ggttaggacg ctgacgctca actggaaaca gccagctgca aataccgtgg
      541 ctataaaagc caggatggcc gggccggtcg cgaataagtt gatattatac attttgattt
      601 aacctttagc gtcttattca aatatagcac tttagtcgac tagaggtcaa aacagagagc
      661 caaaaagttg ttgacaaatc taagcgtttg cgttatagta aaaccataaa ttaatcaaaa
      721 aacaaaaaat ataaaaagga acccaccacc atggaggcaa tactcgataa agttgtcggc
      781 tctagcactt ggcaccgtgc actcgacagc cacgatctac aaaattttgt cgctcgtcat
      841 gagcaggtat tagatggcgt cgacgacgaa gagctgagtg actcagcctt cgacctaggc
      901 ggcctagtcg aataaattat tttctgctac aatcgtctct gttcttttga ctataacccc
      961 taggcagcta agggaaaaga gcagcgctgc ttgcccttta gggtcggctt agccgacagc
     1021 gcgatgagaa aaaccgtgag cttgtctcat tacggcaccg tagctcagct ggttagagcg
     1081 taggactcat aagcctaagg tcactggttc aagcccagtc ggtgctacca aggaaagaac
     1141 cccacctcag tggggctttt tctttggtta gatttactat tgcttgaacc agtgaaaagt
     1201 gagcaaggcg aacggctggt cgtggagcaa gctagcgcgc agaagtttac ttcgagcact
     1261 agtgagcgga aaaggcttta gccgctagcc cagtcggtgc taccaaagaa aaatgatcac
     1321 tacgtgtggt cttttttctt tgatctggtt cataataggg tgcagtatga gtaggaaacc
     1381 tatgttggta gtggccgttt ccggcggcgt tgattcgatg gtcatgctcg ataaactaat
     1441 caagcaatct gaccataacc tagtagtggc tcacgtcaat cacggcattc ggtccgatag
     1501 cggagaagac gaagctctag ttcgacaggt tgccgaaagt caccatctgc cggtagaggt
     1561 ttgtcgactg catttaggcg aaggcgctag tgaacaagtg gctcgacagg ggcgacacga
     1621 gttcttaaac aaagtaaaaa ccgactatgg tgccgaggct gtcgcgaccg cccaccactt
     1681 agacgatgtg gcggaaacca tcatattgaa ctttatgcgg ggtaccggct ggcgtgggtt
     1741 atgctctcta cgcgagacaa acagtcttaa gcggccgctc ttggacttaa gaaaaagcca
     1801 agtcctctcc tatgctatcc aaaacggtct tcactggcac gaagattcga ctaacgacga
     1861 tctgagatac actcgcaatc ttatccgtca cttttatctg tcaaagttac agcccgacca
     1921 gttcgacggc ctgattgcgc tgtggcgtcg tcaatgtatt ttgcgaccgc agatcgatga
     1981 acttgtcagc tctctagtcg agtcgaattt cgaccggtgc tggctggaac tagatgacgc
     2041 catgacggtt gagttactca gggcttggct gggttccaac taccaagccg ccaccttcaa
     2101 gcgacttgta aagtttggcc gagaggcttt gccgggtaaa aagttcaatt tgccgggtga
     2161 tgaaatgatc ttggccgaac gagacaagct cgttgctatc ggacgccgtt aactgctaaa
     2221 atagatgata gcttttttag gtaacaactc ttaaattaaa ggatttattt atgccgatca
     2281 aaactcctcg ccgacccaag cgtccatcat ccaatggttt tcgaacagtt gtcttttggg
     2341 caattattat ttttgtagct ctcggtatca tcggttactt aaccagtggg ccgaaccttg
     2401 agaaagtacc tttctcacag gtaatcaacg aagcgaacag tggtaagatc aacaagatta
     2461 ccatagagag caataagcta ttggtcacca ccaaggccca agatgaccag tccaagccca
     2521 cccaggtatc ctacaaggaa gacggaagca gtatttatga gcaagggtta agccaagacg
     2581 cgcccgtaga ggtcgccgtc acaccgccat ccgacacttc taatgctgtt tggaatttgg
     2641 ctaccatcat cgtgccagtt cttatcatcg gtggcttatt ctatttcatg tttaggcaag
     2701 cccaaggcca gtccaaccaa gctatgggct tcggcaaatc caaagccaaa ctatacggct
     2761 cggataagaa gaaagttgtc ttcgaagatg tcgcaggtaa caatgaagcc aaagaagatt
     2821 tggttgaagt cgtcgacttc ttaaagcatc ctaagaaatt ccgctcggtc ggtgccgaaa
     2881 tacctaaagg tgtactactg gttggccctc cgggtaccgg taaaacctta ctcgcccgag
     2941 ctgtggctgg tcaggccgat gtgccgtttt tctcgatatc cggttcggaa ttcgtcgaaa
     3001 tgtttgttgg tgtcggagct tcccgggtgc gtgacttgtt cgagaaggcc aagaaaaacg
     3061 cgccttgtat aattttcatc gatgaaatcg atgccgttgg tcggcgccgc ggctccggca
     3121 tgggcggtgg tcacgacgag cgtgaacaga cgcttaacca ga
//
LOCUS       DASSBW010000019         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_356981, whole genome shotgun sequence.
ACCESSION   DASSBW010000019 DASSBW010000000
VERSION     DASSBW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356981"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFG56_00495"
     CDS             <1..917
                     /locus_tag="VFG56_00495"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET6622398.1"
                     /translation="RSYRDLPVRYFETTTAYRDEKTGELGGLYRVRSLTQDDSHVYAR
                     ADQIEAVVGQLVADVKTFYETLGLKVRARLSYRDDSDQYLGEAELWQSAQATIKQLAD
                     KNQLDYFEAEGEAAFYGPKIDFMATDALSREHQVATVQLDFVQPERFGLEYTAEDGSK
                     ARPVMIHCAILGSVERFLSVYIEHTAGKFPIWLAPEQVRLIQVNDSEPVQRFVDDFMT
                     RANQHGIRIQVDNSNESVGKKIRLSQVMKVPYSLVIGDKEIETGKLAPRIRGDLAVEG
                     RPESAYGVDQFLNSLANEIRGRVSKSSL"
     gene            920..1225
                     /locus_tag="VFG56_00500"
     CDS             920..1225
                     /locus_tag="VFG56_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET6622399.1"
                     /translation="MSNSSFKDRVYELVVQIPEGRVMTYGDIALLAGHPYAARQVGGL
                     AHFGPTDLPWQRVVNRHGGLASGYYGGKEGHQAALVAEGVEIKDYKIVDFDKRRWRP"
     gene            1246..2130
                     /gene="miaA"
                     /locus_tag="VFG56_00505"
     CDS             1246..2130
                     /gene="miaA"
                     /locus_tag="VFG56_00505"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET6622400.1"
                     /translation="MSNTHPPLIVITGPTASGKTALAVKLAEHYGGEIICADSRTVYK
                     DLDIGTAKPTPVEQARVRHHLLDLVRPDQRFTLKDFQGLAQAAIDDIRSRDKVPFLVG
                     GSGLYIDSVILKYQLDDGAVDLTRRAELESLSIEDLQRLLDENNIDQPSNSLNKRQLI
                     RAYERQGINNQRLATPDDSTVVVQIATDKIELENRIIHRAGQIFSSGVVEEARAAASK
                     YGWDNPAMSGNIYPIIHDLVDGSIDIEEAKRRFIRSDIRLVKKQLTWTRRHKFVHSLS
                     LREAEEFLSREIEHRSRC"
     gene            2173..2754
                     /locus_tag="VFG56_00510"
     CDS             2173..2754
                     /locus_tag="VFG56_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET6622401.1"
                     /translation="MIDALFGSKTRVKLLHLFLSNPNRAFYVREITRKIDEQINSVRR
                     ELANMLSVGIISSESSNNRLYYEVNQDYQHYEPLRAIFVDAELASEAVEASDWSKRLK
                     PLGDVRVAILAGSLVHGSKSDIDVLIGGNVNKTQSKKFFKELEAEEGRPLNYTTMSYE
                     DFYYRLSVKDKFISSVLAGKHTVLVDTENILKV"
     gene            2765..3394
                     /locus_tag="VFG56_00515"
     CDS             2765..3394
                     /locus_tag="VFG56_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6622402.1"
                     /translation="MEIEYKGGNAVVISTKKLDVLIDPKLSQLGLKDVTSKHGCVVAT
                     QAEFVPKDTAGSVVIDGPGEYEVDDISINGVAAERMIDHDGSKQSTIYRVATSDVTVA
                     VIGHSSCPLSEQQLETLSVVDIVVVPVGGNGYTLNANQAVEVVRQLEPKVVIPTHYAD
                     KAAKYEVPQDELELFTRELAADVQELPKLKIKNCQLPEVLTVYKLTRVA"
BASE COUNT          939 a          753 c          899 g          818 t
ORIGIN      
        1 ctcggtctta tcgcgacctg ccggtgcgct acttcgagac gacgacggca tatcgcgatg
       61 aaaaaacggg tgaactgggc ggcctgtatc gagttcgctc tttgacgcag gacgatagtc
      121 atgtctatgc tagggctgat cagatagaag cggtagtcgg ccagctagta gccgatgtaa
      181 agacttttta tgagacattg ggacttaagg tccgggcgag gctaagctac cgtgatgatt
      241 cggatcaata cctgggtgag gctgagctgt ggcagtcggc tcaggctact atcaagcaat
      301 tagccgataa gaaccagctt gattatttcg aagccgaggg tgaagcagcc ttttatggtc
      361 cgaaaatcga ctttatggca accgatgcct taagcagaga gcatcaagtc gctacagttc
      421 agctggattt cgtccagccg gagcggtttg gtctcgaata cactgccgaa gatggctcta
      481 aggctcgacc ggtgatgatc cactgcgcca tactgggttc ggtcgaacgg ttcttgtcgg
      541 tctacattga gcacaccgcc ggtaaattcc cgatatggct agcgccggag caggtcagac
      601 tgatccaggt taacgattct gagccggtcc agagatttgt cgacgacttt atgactagag
      661 ctaaccaaca cggtattcga atccaggttg ataactctaa tgaatcggtt ggtaagaaaa
      721 tccgcttaag tcaggtcatg aaagttcctt atagcttggt tatcggtgac aaggaaatag
      781 aaaccggtaa gctagcccct agaatacgcg gcgacctggc ggttgaagga agaccggaat
      841 cggcttacgg cgtcgaccag tttctcaata gcttggccaa tgaaatacgc ggtcgagtta
      901 gcaagtcatc gctctaaaga tgtccaacag tagcttcaag gatagagttt atgagctggt
      961 agtccagata ccggaaggtc gggtcatgac ttatggcgat atcgctctac tcgctggtca
     1021 tccgtatgcc gcaaggcagg ttggcggctt ggctcatttt ggcccgactg atttgccgtg
     1081 gcagcgggta gtcaatcgtc acggcggctt ggccagcgga tattacggcg gcaaggaggg
     1141 gcatcaggcg gccctcgtcg ccgaaggagt cgagataaaa gactataaaa tcgtggattt
     1201 tgataaacgc cgctggagac cctaatgttt actcttcagc atccaatgtc taatacccat
     1261 cctccactaa tcgtcatcac cggcccgaca gccagcggca agacggcttt ggcggttaag
     1321 ttagccgaac actacggtgg tgaaattatt tgcgccgact ctaggactgt ctataaggac
     1381 ctggacatcg gcacagccaa gcctacacca gtagaacaag ccagagttcg acatcactta
     1441 ctggaccttg tgcggcctga tcagcgcttc accctcaaag attttcaagg tttagcacag
     1501 gccgctatcg acgacattcg gtcgcgcgat aaggttccgt ttttggtcgg cggtagtggg
     1561 ctgtatatcg acagcgtgat attgaagtat cagttagacg atggcgcagt agaccttact
     1621 aggcgagcag agctggagag cttgagtatt gaggatctgc agcggttact ggacgaaaac
     1681 aacatcgatc agccttccaa ctcgttgaac aaacgacaac taattcgagc ttatgagaga
     1741 caaggtataa ataatcagcg acttgccacc cctgacgatt ctactgtcgt agttcaaatt
     1801 gctacagata aaatcgagct agagaatcga attattcacc gggccggaca aatattttca
     1861 agtggcgtag tcgaagaggc tagggctgcg gctagcaaat acggctggga taatccggcc
     1921 atgagtggca atatttaccc gatcatccat gacttagtcg acggcagtat cgacatcgag
     1981 gaagctaagc ggagatttat cagaagtgac atcaggctcg ttaaaaagca gctgacttgg
     2041 actagacgtc acaaatttgt tcactcgctt tctttaagag aggctgaaga atttttaagc
     2101 cgagaaattg aacatcgctc tagatgctaa attgtgctag gatagtactc gaaggaaacg
     2161 aggaaaagac aaatgattga tgcactcttt ggttcgaaaa cgcgcgttaa gctgctgcat
     2221 ttattcttat ctaatcctaa ccgggccttt tatgtccgtg agattactcg aaaaattgac
     2281 gaacagatta attcggttag gcgtgaactg gccaatatgc tgagtgtcgg cattatttcc
     2341 agtgagagtt ccaataaccg gctatactat gaagtcaatc aagactatca gcactatgag
     2401 cctttaagag ccatctttgt cgatgccgag ttagcttctg aggcagtgga agccagtgac
     2461 tggtcgaaac gacttaagcc actaggcgat gtccgggtag caatcttggc cggtagtttg
     2521 gttcacggtt caaagagcga tatcgatgtt ttgattggcg gcaatgtcaa taaaactcag
     2581 tccaagaagt tctttaagga attagaagct gaagaaggcc ggccactcaa ttacacaacg
     2641 atgagctatg aagactttta ttatcgattg agtgtcaaag ataaatttat ttccagcgtc
     2701 ttagccggta agcatacggt gcttgtcgat accgaaaaca tactgaaagt ataaggagag
     2761 ttcaatggag atcgaatata aaggcggtaa tgcggttgtc atcagtacta agaaactaga
     2821 tgtattgatc gacccaaagc tatcacaact aggcctaaaa gatgtcacca gtaagcacgg
     2881 ttgcgtagta gctactcaag ctgagtttgt accgaaggat acagcaggta gcgtggtaat
     2941 tgatgggccg ggtgaatacg aagttgatga tatttcaatc aacggcgtgg cggctgagcg
     3001 aatgattgat cacgacggca gcaagcaatc tactatttac cgcgtagcca ctagcgacgt
     3061 tacggtggcg gtaatcggac acagttcatg tccgctcagc gaacaacaac tagaaaccct
     3121 cagtgtggtc gacatagttg tggtgcctgt tggcggcaat gggtatacgc ttaacgccaa
     3181 tcaggctgtc gaggtagttc gtcagctgga gcccaaagta gtcatcccaa cacactacgc
     3241 cgataaggct gccaaatacg aagtgccgca agatgagcta gagctgttta ctcgtgaact
     3301 ggcagctgat gtccaagaac tgcccaagct aaagatcaag aactgtcagt tgccggaggt
     3361 actaactgtc tacaaattga ccagagtggc ttaaagaaaa agacgatta
//
LOCUS       DASSBW010000020         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1273830, whole genome shotgun sequence.
ACCESSION   DASSBW010000020 DASSBW010000000
VERSION     DASSBW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273830"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFG56_00520"
     CDS             <1..839
                     /locus_tag="VFG56_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6622403.1"
                     /translation="ALGEATTMTTIINTEIDVCDPAEPQKFSITKGRVVFDNVSFAHP
                     DDKKDVLFTKLNLDIKAGQKIGLVGHSGSGKTTLTKLLLRFMDTDTGSITIDGHNIRD
                     IAQTDLRSSIAYVPQEPLLFHRTLAENIAYAKPDASLEEIRSAADKANALEFIERLPH
                     GFDTVVGERGIKLSGGQRQRIAIARAILKDAPVLILDEATSALDSESEKSIQQALSQL
                     MKGRTAIVIAHRLSTIRKLDRIIVMETGMIREDGDHQQLIDQGGIYAHLWHHQSGGLI
                     EQ"
     gene            complement(884..1669)
                     /locus_tag="VFG56_00525"
     CDS             complement(884..1669)
                     /locus_tag="VFG56_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET6622404.1"
                     /translation="MGDKVNLKLTKREVHGKKVKNLRRDGLVPGVVYGPGTEPIAVQA
                     PDNLVAKAYREAGRHHPVYLDIEGKKKIAMIKHADVDPVKNNLRHLSFHAVKQNEKVE
                     AEIPVRLKGEGESEAEKAGLIILQNIEELEVRALPMDLPDFLEADITGLKESGERVTV
                     GDIKLGAGIELVDKVTQQKADADDEDDDEQEHSVTELVVASVWEPSALQAANEAAAGD
                     AEDESEVESENGEDTDQESQAGEDKPGGKDQAEPKQSNVDANK"
     gene            1774..2493
                     /locus_tag="VFG56_00530"
     CDS             1774..2493
                     /locus_tag="VFG56_00530"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6622405.1"
                     /translation="MRKYLTPLIALTVFVLGGLAYLAIDRYGLNALISLIGVAGVAAI
                     IFAESGLLIGFFFPGDSLLFTAGFLVHSGDLQFNIHWLAVIVFIAATLGDNVGYSFGS
                     RVGHRLFRRPDSLIFHKDNLKRAEAFYDKHGAKTILLARFVPVVRTFAPIVAGISKMR
                     YRTFLSFNLIGAFVWAIGLTYLGFFAGSWIDKLGINIELVLILIVFLSILPPVIHVLK
                     EPASRQQLKNFLGQLKNRLLK"
     gene            complement(2482..>4137)
                     /gene="uvrA"
                     /locus_tag="VFG56_00535"
     CDS             complement(2482..>4137)
                     /gene="uvrA"
                     /locus_tag="VFG56_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6622406.1"
                     /translation="SEFMRRDIERFMRERPCHACNGKRLKPEVLAITVDDLSIMDICG
                     LSIDDALELFDKNLKLDEQSTQISTQIFKEICARLGFMHAVGLNYLSLARAANTLSGG
                     EAQRIRLATQIGSGLQGVLYVLDEPSIGLHQRDNDRLISTLKNLRDLGNTVLVVEHDE
                     DTIREADYLVDIGPGAGVHGGQVQAAGTPTEVAKMDVSITGRYLSGKEKIEVPKKRRS
                     GDGRQLVIKGATEHNLKEVEAAIPLGVLTVVSGVSGSGKSTLINDILAKELAARLHRS
                     QEVAGAHKDIEGIDQLDKVIVIDQSPIGRTPRSNPATYTGVFTPIRELFASTPEANVR
                     GYKAGRFSFNVKGGRCENCQGDGVIKIEMHFLPDVFVTCEVCHGKRYNREALEITYKD
                     KTISDVLEMTVEQAADFFANIPVIAKKMETLREVGLGYIRLGQPATTFSGGEAQRIKL
                     ATELSRRATGKTLYILDEPTTGLHSADVKRLLSVLQQLVATGNSMVIIEHNLDVIKSA
                     DHIIDMGPEGGDKGGQVVAEGTPEQVAKNPASYTGKYLGKLLK"
BASE COUNT          967 a         1087 c          977 g         1106 t
ORIGIN      
        1 gggcccttgg ggaagccact accatgacga caattataaa taccgagatc gacgtgtgtg
       61 atccagccga accacaaaag ttcagtatca cgaaaggtcg agtagttttt gataacgtca
      121 gtttcgcgca tcctgatgac aaaaaggatg tcttgtttac caagctgaat cttgacatta
      181 aggccggtca gaaaattggt ctggttggac actccggttc gggtaaaaca actttaacca
      241 agcttctact gcgcttcatg gacaccgata ccggctctat cactatcgat ggtcacaata
      301 tccgagacat cgcccaaacc gaccttcgct cctcgatcgc ttatgtaccc caggagccgc
      361 tactatttca ccgaacttta gccgaaaata tcgcctacgc caaaccagat gctagcctgg
      421 aagaaatccg gtcagccgcc gacaaagcca acgcactcga gtttatagaa agattgccgc
      481 acggttttga tacggtggtc ggcgagcgag gtatcaagtt gtccggcggt caacgccagc
      541 ggatagctat cgctcgggcc atcttaaagg atgcaccggt attgattctg gatgaggcta
      601 ccagcgcctt agacagcgaa agcgaaaaat cgatccagca agccctgtct caactcatga
      661 agggacggac ggccatagtc atcgcccatc gccttagcac tatccgaaaa ctggaccgga
      721 ttatcgtcat ggagaccggt atgatacgtg aagatggcga ccaccagcag ctgattgatc
      781 aaggtggaat ttacgctcac ctatggcatc accaatccgg cggattgatc gaacaataaa
      841 acaccgcccg accgggcggt gttttggaac ctaaatagcc gagttatttg ttggcgtcga
      901 cgttggactg cttcggttcg gcttgatcct taccgcccgg cttgtcttca ccggcctgag
      961 actcttgatc ggtatcctca ccattctcag attcaacttc cgactcatct tcggcatcgc
     1021 cagcagcagc ttcgttagcg gcttgcaagg cacttggctc ccaaacacta gccacaacca
     1081 gttcggttac actgtgctct tgttcgtcat cgtcctcatc atcagcgtca gccttttgtt
     1141 gagtaacctt atcgactaat tcaatcccag ctcccagctt aatgtcaccg accgttaccc
     1201 gctcaccgga ttctttaaga ccggtgatat cggcttccag gaagtccggc agatccatag
     1261 gcaaggcccg tacttctagt tcctcgatat tttgtagaat gatcaaacca gctttttcag
     1321 cctcggattc accttcaccc tttaggcgga cagggatttc agcttccact ttctcgttct
     1381 gcttgacggc gtggaacgac aaatggcgca ggttgttttt aacaggatca acatcggcat
     1441 gcttgatcat ggcgattttc ttcttacctt caatatccaa gtacactggg tggtgtcgac
     1501 cagcttcacg gtaggcctta gcgactaggt tatcgggtgc ttgaacagcg atcggttcgg
     1561 taccaggacc gtagactacg cccggcacta ggccgtctcg acgcaaattt ttaacctttt
     1621 taccatgcac ttcacgctta gttaacttca gatttacctt atctcccatc tctctcctct
     1681 cggatacttt tacggagcat ctcgtaatta gttagtacta cctattataa caaactaggc
     1741 tttttgcaga ccgatagagt aaacttggtt aagatgagaa aatacctaac accgctgatt
     1801 gccttaactg tattcgtcct tggcggtctg gcttatctag cgattgatcg ctatggcctg
     1861 aatgctttga tttcactaat cggcgtggct ggtgtggccg ccatcatctt tgccgaatcc
     1921 ggacttttga tcggcttttt ctttcccggt gatagtttac tctttacggc cggcttcttg
     1981 gttcatagcg gtgacctgca gttcaatatc cactggctag cggttatcgt ctttatcgcc
     2041 gccacactgg gtgacaatgt tggctatagc tttggttctc gagtgggtca ccgcttgttt
     2101 aggcgccctg attcattgat ctttcataag gataacttaa aacgcgccga ggctttctac
     2161 gataagcacg gcgctaaaac tatcttgcta gcacgcttcg taccggtggt ccgaactttt
     2221 gcgcctatcg tcgccggtat cagcaagatg cgttatcgga cttttcttag cttcaacctg
     2281 atcggtgctt ttgtttgggc tatcggccta acctatcttg gctttttcgc cggtagctgg
     2341 atcgataagt tgggtatcaa tatcgagttg gtgctaatct tgatcgtctt tttgtctatc
     2401 cttcccccgg taattcacgt gctcaaagaa ccggctagtc gtcaacagct aaagaacttt
     2461 cttggtcagc tcaagaaccg actacttaag tagcttgcct agatacttgc cggtgtagct
     2521 ggctgggttt ttagcaacct gctcgggcgt accttcggca acgacctgac cacccttatc
     2581 gccaccttcc ggccccatgt cgatgatatg gtcggccgat ttgataacgt ctaaattgtg
     2641 ctcgatgata accatagaat taccggtagc caccaattgc tgaagcacgc taagtagccg
     2701 tttgacatcg gctgaatgaa gaccggtcgt cggttcgtca agtatataga gagtcttgcc
     2761 ggtagcccgg cgagacaatt cggtggctaa tttgatccgc tgagcttcgc cacccgagaa
     2821 ggtggtcgcc ggctggccta gccggatata gccaaggccg acttcgcgca aggtttccat
     2881 ctttttggcg ataaccggga tattagcgaa gaagtcagct gcttgctcga cggtcatttc
     2941 cagtacgtca gagatggtct tatccttgta tgtaatctct agggcttcac gattataacg
     3001 cttgccgtga cagacttcac aggtaacgaa tacgtcgggt aggaagtgca tctcaatctt
     3061 gatgacacca tcaccttgac agttctcgca tctaccacct ttaacattga agctgaaacg
     3121 accagccttg tagccacgaa cgttggcttc gggtgtcgaa gcgaacaact cacggatagg
     3181 tgtgaaaact ccagtatagg tcgccgggtt gcttctgggt gttcgaccaa tcggactttg
     3241 atcgataact atgaccttgt cgagctgatc gataccctcg atatctttat gagcaccagc
     3301 cacttcttga gaccggtgga gacgagcggc caattccttg gctagaatat cgttgatcag
     3361 ggttgatttg ccggaaccac taacgccaga aacgacagtt agaacaccga gtggaatagc
     3421 cgcttcaact tcttttaggt tatgctccgt ggcgccctta atcactagtt gtcggccatc
     3481 accggagcga cgctttttcg gtacttctat tttttcttta ccgcttaaat accggccagt
     3541 gatcgagaca tccattttgg caacttcagt tggagtgccg gcggcttgaa cttgaccgcc
     3601 atggacaccg gcgccgggac ctatatcgac cagatagtcg gcctcacgga tagtatcttc
     3661 gtcgtgctca accacaagaa ctgtgttgcc taggtcgcgc agatttttca aagtggaaat
     3721 cagcctgtcg ttgtcgcgct gatgcaagcc gatcgaaggt tcatcaagta cgtacagaac
     3781 accttgtagt ccggagccga tttgagtggc caggcgtatc cgctgagctt caccgccgga
     3841 tagcgtgtta gcggcccgag ccagcgataa gtaattaaga ccgacggcat gcataaagcc
     3901 gagcctggcg caaatttcct taaagatctg tgttgaaatc tgagtcgatt gttcgtctag
     3961 ctttaaattc ttgtcaaata actccaaggc atcgtcgata ctcagcccac agatgtccat
     4021 gatactcaaa tcatcaacgg tgatagccaa gacctctggc ttcaagcgct taccattgca
     4081 agcatgacac ggacgttcgc gcataaacct ctcgatatca cggcgcatga attcact
//
LOCUS       DASSBW010000021         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_284770, whole genome shotgun sequence.
ACCESSION   DASSBW010000021 DASSBW010000000
VERSION     DASSBW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284770"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..393
                     /gene="rpsJ"
                     /locus_tag="VFG56_00540"
     CDS             70..393
                     /gene="rpsJ"
                     /locus_tag="VFG56_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET6622407.1"
                     /translation="MADAKTEAPRIRIRLKAYDHKVIDQSAKQIIDTALRTGAQIAGP
                     VPLPTRRSTYTVVKSPHVYKQGGETFEMRVHKRLIDIIGATPKTIDSLQNLSLPAGCD
                     AEIRM"
     gene            492..833
                     /locus_tag="VFG56_00545"
     CDS             492..833
                     /locus_tag="VFG56_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET6622408.1"
                     /translation="MSKNKLKLTAVSGAAALAGIVTGFLIAPKSGKQTRQELRDQAEQ
                     LKAQAKHKKHNFRGALGYSSNEIKDSLRRSAREMSDLAEEAKLRASRIADDARKTGHV
                     VTGDLRHRLRD"
     gene            complement(830..1525)
                     /locus_tag="VFG56_00550"
     CDS             complement(830..1525)
                     /locus_tag="VFG56_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6622409.1"
                     /translation="MLPGVDLPLPRSRDPSRLTGGSIGRFTRDWPVKNYTRHQLIIQP
                     DDQLNSLYLIKSGFVREFDISAHGDEHVLWYGRPGDIFATPRLFDESYRCHHFYDAFS
                     DCQLRVIDAKSLSDYVEGNTRALVQLTKYLAADRDSAEMRLQALAEPKASAKVLRILE
                     FFARRFGTVNRRRQVSVRLALTHQDIANFLGLTRETVSHELNKLRELGVVDYHNADIS
                     VDVAKLSKLLSDA"
     gene            1555..1839
                     /locus_tag="VFG56_00555"
     CDS             1555..1839
                     /locus_tag="VFG56_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622410.1"
                     /translation="MPTKNKTKKSTAKTEADGSFLLKVVLYAILGSLWLKFSQPIDLG
                     SFSLRGLPVGLVVGLIFASHDHFQVDRKLEYVILIIMTVLSFFVSSGIVI"
     gene            2004..2618
                     /gene="rplC"
                     /locus_tag="VFG56_00560"
     CDS             2004..2618
                     /gene="rplC"
                     /locus_tag="VFG56_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET6622411.1"
                     /translation="MKTLIGTKIGMTQVVSEDGVALPVTLIQAGPVTVTQVKTVEKDG
                     YSAVQLGYGKGKNLSNAVAGHVKQAKITPKVIREVRLDELSDDLTVGANFDVSVFTVG
                     DVVDVTGTSKGKGFAGTVKRHNFNTSKKTHGGNGNVRKPGSIGSMYPQKIFKGKRMAG
                     RMGHERVTVKNLLVAYVDQELGLIGLKGAVPGPKKGIVRIGGKA"
     gene            2618..3268
                     /gene="rplD"
                     /locus_tag="VFG56_00565"
     CDS             2618..3268
                     /gene="rplD"
                     /locus_tag="VFG56_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET6622412.1"
                     /translation="MAETKTTKPTLKTDIFGLKVENHQLLKTAYEAYLAEARGANATT
                     KRRGEVRGGGKKPWKQKGTGRARVGSSRNPIWRGGGIVFGPLGIENYTKRLSTKSKRT
                     ALRQALSLAKAAGKIELLDFKATGKTKDAAKFLADNKLDRRVLIVVDQKTTEMSRSVN
                     NLPETELISARYLTVYQILNADKIVLTPKAVDVINQWLGTASPRSTASKAIEKEAK"
     gene            3268..3570
                     /gene="rplW"
                     /locus_tag="VFG56_00570"
     CDS             3268..3570
                     /gene="rplW"
                     /locus_tag="VFG56_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET6622413.1"
                     /translation="MLISPRMTEKAYATSADGTYVFNVPMSANKQTVAAELKDQFKVD
                     ATDVRFVVAKGKTRRTYRSSRKNPVVSVGKDKKKAYVRLAEGQSLDLFKSEDEEKK"
BASE COUNT          958 a          864 c          945 g          828 t
ORIGIN      
        1 attcgaatca aggttacgag atcgctaggc cgccagcgtt aagggacggc aaataggaga
       61 aataccacta tggcagacgc caaaactgaa gctccgcgga ttcgtatccg cctgaaagct
      121 tatgaccaca aggttatcga ccaatcagcc aaacaaatta tagacaccgc gcttcgtacc
      181 ggtgctcaga tcgccggtcc ggtaccactg ccgactcgcc gatcgaccta cactgtcgtc
      241 aaaagcccgc acgtttataa gcagggcggt gagactttcg agatgcgcgt tcacaagcgc
      301 ctgatcgaca tcatcggcgc tacaccgaag accatcgact ctttgcagaa cctgtcgcta
      361 ccggctggct gcgatgccga aattcgtatg tagtctaaat cgcataaaaa tactaaccgc
      421 cgtgaaataa ctcacggcgg tttagttttt tgtacctata ctggattgac gaagactact
      481 agggaggtgg tatgagcaaa aataagttga agttgacggc tgttagtggc gcagcggcat
      541 tggccggtat agtgactggt ttcttgattg cgccaaagag tggcaagcag actcgacaag
      601 aattgcgcga tcaagccgag cagctgaagg cccaggctaa gcacaagaag cataactttc
      661 gcggtgcact cggctatagc agcaatgaga tcaaagatag cttacgtcgg tcggctcgag
      721 aaatgagtga cttggccgaa gaagctaagc taagggccag tcgtatcgcc gatgatgccc
      781 gtaaaaccgg gcacgtggtc actggcgacc tgagacatcg cttgcgcgac taagcgtcgc
      841 ttaagagctt ggatagttta gctacgtcaa cgctgatatc agcgttgtgg taatcaacta
      901 caccgagttc cctaagctta ttgagttcat gcgagactgt ctcgcgcgtt aggccaagga
      961 agttggcgat atcttggtgg gttagcgcca accttacaga tacttggcgg cggcggttca
     1021 ccgtaccgaa acggcgggca aagaattcga ggatgcgcag aaccttggcg cttgccttgg
     1081 gctcggctaa agcctgtaga cgcatctcgg cgctgtcgcg atcggcagcc agatacttgg
     1141 ttagttgaac tagcgctctg gtgttaccct cgacatagtc gcttaggctt ttggcatcaa
     1201 tcactctaag ctggcagtca gagaaggcgt cataaaagtg gtggcaacgg tagctttcat
     1261 caaataagcg gggtgtagcg aagatgtcgc cgggtcgacc gtaccacaag acgtgttcgt
     1321 caccgtgggc gctgatatcg aattctcgga cgaaaccgga cttgatcaga tagagagaat
     1381 tcagttgatc gtcgggctgg atgataagtt gatgtcgggt gtaatttttg accggccagt
     1441 cacgggtaaa acggccgata ctaccaccgg taagccgact tggatctcgg ctccgaggaa
     1501 ggggtaaatc tacgcccggt agcataagtc tgattatacg gtatacttga ggtaatgcct
     1561 acgaaaaata aaactaaaaa atcgaccgct aaaactgagg ccgacggcag ctttcttcta
     1621 aaggtagttt tgtatgccat cttaggttcg ttatggctca agttttccca gccgattgac
     1681 cttggcagtt tcagtctccg aggcctgccg gtcggcctgg tcgtcggtct gatctttgcc
     1741 agccacgacc attttcaggt agatcgtaag cttgagtatg tcatcttgat catcatgacg
     1801 gtgcttagtt ttttcgtctc tagcggcatc gtcatatagc caaatagctt atagaggtaa
     1861 aaccccttga ctttttaagg tgtttcgtgg tacatttgtt tggtaactta ttacgtaata
     1921 tcaactcgtc ttggtgaatg agcaggtcct gccaaaagga aaccgctcgc caccaagaat
     1981 tttaagtggg aaaagggaag caaatgaaga ccctcatcgg taccaaaatt ggtatgaccc
     2041 aagtcgttag cgaagacggc gtagctcttc cggttacgtt gatccaagct ggccctgtga
     2101 cagtcactca ggtgaagact gtcgagaaag acggctacag cgccgttcaa ctgggctatg
     2161 gcaagggtaa gaacctgagc aatgccgtgg ctggccacgt aaagcaagcc aaaatcacac
     2221 caaaagtcat cagggaagtc cgtttggatg aattgtctga tgatttaact gtcggcgcta
     2281 actttgacgt tagcgttttt accgttggcg atgtcgtcga cgtaactggc acttcaaaag
     2341 gtaaaggctt tgccggcacc gttaagcgcc acaacttcaa cacttctaag aaaacccacg
     2401 gtggtaacgg caacgtccgt aagcctggtt cgatcggctc aatgtaccca cagaagatct
     2461 tcaagggtaa gcgcatggct ggccgaatgg gtcacgagcg cgttaccgtc aagaatctac
     2521 tggtagctta tgtcgatcaa gaacttggtc ttatcggcct aaaaggcgcc gtacctggac
     2581 cgaaaaaggg catcgtccgt atcggaggta aagcataatg gctgagacta agactactaa
     2641 accaaccttg aagactgaca ttttcggcct taaggtagaa aatcaccaac tactgaagac
     2701 cgcttatgag gcatacctgg ctgaggctcg tggcgctaac gctacgacta agcgacgtgg
     2761 tgaagttcgc ggtggcggta agaagccatg gaagcaaaag ggcaccggcc gagctcgagt
     2821 aggtagttca cgtaacccaa tctggcgcgg tggcggtatt gttttcggtc cacttggtat
     2881 cgagaactac accaaacgac ttagtactaa gtccaagcgt acagccctac gccaagctct
     2941 aagccttgct aaagctgctg gcaagatcga actactagac ttcaaggcta ctggtaagac
     3001 taaagacgct gctaagttcc tggctgacaa caaactggat cgccgggtcc tcatcgtggt
     3061 tgatcaaaag acgaccgaga tgtctcgttc ggtcaacaac ctgccagaga ctgagctaat
     3121 cagcgcccgt tatctaactg tttatcaaat tttaaacgcc gacaagattg tcctgacgcc
     3181 taaggctgtc gatgtaatca accaatggct cggtacggca tccccgcgaa gcaccgcttc
     3241 taaggcgatc gagaaggagg ctaagtaatg ttgatctccc cacgaatgac cgaaaaagcc
     3301 tacgccacta gtgctgatgg tacttacgtc ttcaacgttc caatgagtgc caacaaacag
     3361 actgtcgcgg ctgagcttaa agatcagttc aaggttgatg ctactgacgt tcgttttgtt
     3421 gttgccaagg gtaaaacccg tcgaacttac cgttcgtctc gcaagaaccc agtcgtatcc
     3481 gttggtaaag acaagaaaaa agcctacgtt cggcttgctg aaggccagag cctggatctg
     3541 tttaagtcag aagacgagga gaagaagtaa tggcgatcaa gcaatacaaa gctac
//
LOCUS       DASSBW010000022         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_945177, whole genome shotgun sequence.
ACCESSION   DASSBW010000022 DASSBW010000000
VERSION     DASSBW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945177"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFG56_00575"
     CDS             complement(<1..442)
                     /locus_tag="VFG56_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622414.1"
                     /translation="MDIRSSFNNVVDTIVNWIPNLIEFVLIVIVGYFVALLFDWLVRT
                     LLRLSRLDEYLERHRAGQWVTHVLGSPSNFLGKVVFWVVWFGSFGVAAALADVPLVGD
                     LVSAVYSYIPNVLSALFIFVFAALISGAVVTVVNRTMGDTPTGKV"
     gene            557..1570
                     /locus_tag="VFG56_00580"
     CDS             557..1570
                     /locus_tag="VFG56_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HET6622415.1"
                     /translation="MQLFHAHSPYRIFGFSGFITIAGLILALYLFGWSALITTLILIV
                     IEVTFSFDNAIINAKILERLSKPWQTAFLTIGILIAVIGMRLVFPIVIVSLSAGLGLA
                     EVVNLALNQPESYSAKLDQSHTLIASFGGAFLLMLTLGFFFDRAKPTHWWNRFEKPIS
                     RLGNNWWPTIITLLVVVALSSVGGQIDQRILEAGVIGIGVYLMVHIGSELLNRRSNHR
                     GPATGWAAFMLFIYLQVIDASFSFDGVIGAFAITSDVILIALGLGVGALWVRSLTIFM
                     VRRGTLKNYIYLEHGAHYTIGVLAIAMFVSLFWHLPEAITGLIGIGIIGSSIAASVQA
                     KRA"
     gene            1690..2916
                     /locus_tag="VFG56_00585"
     CDS             1690..2916
                     /locus_tag="VFG56_00585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6622416.1"
                     /translation="MPNNSFRAAKRPHNNNFKRGRRSGGNTRGPKKQSIHPSKFINRA
                     VERGETEVYQPINKFSDFALNTKLQANLAAIKFTSPSAIQDQAIPLALEGHDVIGLAN
                     TGTGKTAAFLLPILEKLNADERRNSVLIMAPTRELAQQIDEEFRRFSTGLKLYSAVCV
                     GGTNIERQIRQLKRQPHVIIGTPGRLKDLLNRRVLRLEHINTFVLDEADRMLDMGFIA
                     DIRFVAGLLPTDRQTLCFSATMTPSIRQLVNDFMRSPETVSVRTGETSDHVDQDVVEA
                     YDKLHKLELLSEMLGKDEFEKVLVFGETKYGVQRLADNLNKADISAEAIHGNKSQSQR
                     QRALKAFKSSKVKVLVATDVAARGLDIPNVSHVINFDQPNTYDDYVHRIGRTGRGGQV
                     GKALTFVEPSHHRFGR"
     gene            3027..>3264
                     /locus_tag="VFG56_00590"
     CDS             3027..>3264
                     /locus_tag="VFG56_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622417.1"
                     /translation="MKLTTKALASLLIMAALTVSSSAGVSAVGPNSPTDRRQDKRFAK
                     LVRRHDRKMELRASVLGMTGDELKEQLRVVPMNQL"
BASE COUNT          849 a          809 c          787 g          819 t
ORIGIN      
        1 cgactttgcc ggtcggtgta tcgcccatgg ttcggttgac gactgttact acggcaccgg
       61 agatcaaagc ggcaaagaca aagatgaaca atgccgatag aacatttggt atgtagctgt
      121 agacagccga tactaaatcg ccaaccagtg gcacgtcagc caatgcggcg gctacaccga
      181 atgagccgaa ccaaaccacc cagaaaacaa ctttaccgag aaaattcgaa gggctgccca
      241 gcacgtgggt tacccactga ccggcacggt gccgttctag gtactcatct aacctggaga
      301 ggcgtaggag cgtacgaacc agccagtcga acagcagggc gacgaaataa ccgactataa
      361 cgatcagtac gaactctata aggttgggaa tccagttgac gatggtatca acaacattgt
      421 taaaacttga cctgatatcc attggagcta cctttcttct aagttaatga ggttctatcg
      481 tcatcataaa ctaaaaaagc actatttatg tgatattttt aacaaactta tgattatctt
      541 ttataatgct aaagcaatgc agttatttca cgcccactca ccttaccgga tatttggctt
      601 ttccgggttc attactatcg ccggtttgat cctggctctg tatttgtttg gctggtcggc
      661 cttgatcact actttgatct tgattgtcat cgaggtgact tttagttttg ataacgccat
      721 tatcaacgcc aagatactag agcggctatc taagccatgg cagacggctt tcttgactat
      781 cgggatccta atcgctgtta tcggtatgcg cctggtcttt cctatagtta tcgtcagctt
      841 atcggcggga ctgggcttag ccgaagtggt taatttggcg cttaatcagc cagaatcata
      901 cagcgccaag cttgaccaat cccatactct gatcgccagt ttcggtggag cttttctgct
      961 gatgctgacg ctcggcttct tttttgaccg ggccaaacct acccactggt ggaaccgctt
     1021 cgaaaaacct atcagccgac tcggtaacaa ctggtggccg actatcataa cgctactggt
     1081 agtggttgct ctatctagcg tcggtggcca aatcgatcag cgcatcctgg aggccggtgt
     1141 tatcggtata ggtgtttatc taatggtgca tatcggctca gagctactta accgacggtc
     1201 caaccaccgt ggccccgcta ccggctgggc ggctttcatg ctattcatat acctacaagt
     1261 catcgacgcc agcttcagct ttgatggcgt tatcggcgcc tttgccatca ccagcgacgt
     1321 catcttgatc gccttaggtt taggtgtcgg cgccttgtgg gtacggtcgc tgactatttt
     1381 catggttcgt cggggtacac taaaaaacta tatctatctg gagcatggcg cgcactacac
     1441 catcggcgta ttggcgatag cgatgtttgt tagtctgttt tggcacctac cagaagctat
     1501 caccgggctt atcggtatcg gcatcattgg ttcatcaata gccgcatcgg tccaagccaa
     1561 acgagcctag acgtactaaa atgtctatgt tataattacg ccagcaatta gtgctggatg
     1621 aatacctgat tttctctaac aagactcttg tcattcatca aataatgaag gcaagaaagg
     1681 gaaatatcca tgccaaataa ctcttttcgt gcggccaaac gaccgcacaa taacaacttt
     1741 aaacgtggcc ggcgaagtgg cggcaataca cgtggtccga aaaaacaatc gatccacccg
     1801 agcaagttta tcaaccgagc cgtggaacgc ggtgagaccg aagtatatca gccgatcaat
     1861 aagtttagtg attttgcgct aaacaccaag ctgcaggcaa atctggcggc aattaaattc
     1921 actagcccta gtgccatcca agatcaggct ataccactag cactggaggg tcatgatgtc
     1981 ataggcctag ccaataccgg tactggcaag acggcggctt tcttgttgcc gatcctagaa
     2041 aagctaaatg ccgatgaacg gcgcaattca gttttgatta tggcgccaac cagggagcta
     2101 gctcagcaga ttgacgagga gtttcgtcgt ttttcaaccg ggcttaagct gtattcggca
     2161 gtttgtgtcg gcggtaccaa tatcgaacgg caaattcgcc agttgaaacg ccagccacat
     2221 gtcatcatcg gtacgccagg ccgactgaaa gatcttctca atcgtcgtgt cctacgactt
     2281 gagcacatca acacttttgt gttggacgaa gccgaccgaa tgctcgacat gggctttata
     2341 gccgatatcc gctttgttgc cggcttactg ccgaccgatc gtcagacgct gtgtttcagt
     2401 gccactatga caccgtcgat caggcagtta gtcaacgact ttatgcgtag tccagaaact
     2461 gtgtcggtac ggactggcga gactagcgat catgtcgatc aggatgtggt ggaagcttat
     2521 gacaagcttc acaagctgga actacttagc gagatgctgg gaaaagatga attcgaaaaa
     2581 gttttggttt tcggcgagac caaatacggc gttcaacggc tagccgacaa tttaaacaaa
     2641 gccgacatct cagccgaagc catccatggc aacaaatcac agtctcagcg ccaacgggcg
     2701 cttaaggcct ttaagtccag taaagtaaag gtcttggtgg ccaccgacgt ggccgcacgg
     2761 ggtcttgata tcccgaatgt gtcgcacgtc atcaattttg atcagccaaa tacctatgat
     2821 gactacgttc accgcatcgg tagaactggc cgtggcggcc aagtcggcaa agccctgact
     2881 ttcgtagaac cgagccatca ccgcttcggt cgttagtatt gttgcctcgc gttgttaaat
     2941 caacgaatca taagcgattt aactcacgta taataaaggc ataaccatcc tgaaactgtt
     3001 ttaaacaaaa gaagggggta agaattatga aactaacaac caaagcgctg gcttcactgc
     3061 ttatcatggc agccttgacc gtcagttcca gcgcaggcgt atcggccgtt ggtccgaact
     3121 ctccaaccga tcgccgtcaa gacaaacgat ttgccaagtt agtccgccgg catgatcgta
     3181 agatggagct aagagcttcg gtgcttggta tgacgggtga cgagctcaaa gagcagctac
     3241 gtgtcgtacc gatgaatcag ctga
//
LOCUS       DASSBW010000023         6785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1173670, whole genome shotgun sequence.
ACCESSION   DASSBW010000023 DASSBW010000000
VERSION     DASSBW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173670"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="opgC"
                     /locus_tag="VFG56_00595"
     CDS             <1..774
                     /gene="opgC"
                     /locus_tag="VFG56_00595"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HET6622418.1"
                     /translation="AHVAKLPTGDQVSSLGAYLLQVFSTQFTYDWIYFLRLYAIIMVA
                     APLFIWAARTRKLAIFLVLSASVYAASFLMEEPEGSLQWQFIFFVAASVGYKLPSIID
                     YLNDHVRLKKWLMYGSIATTIAIFAVSYFFVLAWPDGVLMSRAQYSAIHDVTEVVFSN
                     NPLMPARVALSFAWFAGMISLFHVLKRPIAKYLGWMFAQFGNYSLSVYCLQAVVLIFF
                     NWLVPFTTNFFYNFFINLGLLLIIWLIMRTKLAKNILPR"
     gene            832..908
                     /locus_tag="VFG56_00600"
     tRNA            832..908
                     /locus_tag="VFG56_00600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Pro,seq:tgg)
     gene            995..1071
                     /locus_tag="VFG56_00605"
     tRNA            995..1071
                     /locus_tag="VFG56_00605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1029..1031,aa:Pro,seq:cgg)
     gene            complement(1290..1568)
                     /locus_tag="VFG56_00610"
     CDS             complement(1290..1568)
                     /locus_tag="VFG56_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622419.1"
                     /translation="MAELSPGDIQKAVQAGVGSLHRDISRLSSQIFSLQRDLKKVAYL
                     QQQAAALDKRTFDLVSEIQELKLRISYIERYCAFIYGQYVETEEEQNR"
     gene            complement(1641..2114)
                     /locus_tag="VFG56_00615"
     CDS             complement(1641..2114)
                     /locus_tag="VFG56_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622420.1"
                     /translation="MNMHTLFSNPGKKLILTGVALGISLQAMLWLSHLITERGILGAL
                     VYPLIGIGVIAGISAYLSHRKLTFWTSLFALMFAWPVVKLLYLLLPVSWLAFLLQIAV
                     TGAFFVLAHTALTEWRGKLWPKLSIGVVLFVVWFFTISIAYSYLARRIAAATYFS"
     gene            complement(2121..3629)
                     /gene="rny"
                     /locus_tag="VFG56_00620"
     CDS             complement(2121..3629)
                     /gene="rny"
                     /locus_tag="VFG56_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET6622421.1"
                     /translation="MFEVIFAVVGLLVGAGGVTIVNQARASKKGHKADKLIADAKSKA
                     SDIVLKAKDEAITISKDAQKSDEERRKEIKKSEDRLASRETNLDKKLEDLDKRSEKLR
                     KEEDEIDDLKDEIRDIRTKQQDKLEKIAKLTKKDATEKLMAMTERDIKQDLLGLVSKL
                     QRDTQEDAEERAQTILVSAMERMASDVTAERTVTAIKLEGDEMKGRIIGKEGRNIQAL
                     QRATGVDILVDDTPGMIVLSSFDPVRRQVARLTLEMLMKDGRIHPARIEEVVAKAHKQ
                     IDKDIERAGEDAAREVGVVGVPPELLRLLGELKFRTSYGQNVLKHSTEMAHMAGLIAE
                     EIGANVQVTKMATLLHDIGKAVTHRMEGKHHHIGEELARKYGLSDDIAHAIGAHHDDV
                     EATTPEALIVRVCDALSAARPGARNISAENFVERMKELENVATSFDGIDKAYAISAGR
                     EVRVIVRPKDIDDLSAIKLARDIANKIESTMQYPGTIKVSVIRETRAIEFAK"
     gene            complement(3805..4197)
                     /gene="tsaB"
                     /locus_tag="VFG56_00625"
     CDS             complement(3805..4197)
                     /gene="tsaB"
                     /locus_tag="VFG56_00625"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET6622422.1"
                     /translation="MILAIKTATGVAELRLLSREGQTLAEADWPADRNLADGLLAHIA
                     KMIKREGAVFSDLTGLVVFKGPGSFTGLRIGITVMNTLSYALDIPIVGAASNNWQKKG
                     LHALKDGHNDRLVLPEYGAEARITPPKH"
     gene            complement(4194..4652)
                     /gene="tsaE"
                     /locus_tag="VFG56_00630"
     CDS             complement(4194..4652)
                     /gene="tsaE"
                     /locus_tag="VFG56_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET6622423.1"
                     /translation="MERAITSDVAMQAFGYKLGKLLRGGEVIELVGDLGAGKTTLVRG
                     IADGLEIDEDVQSPSFTLSRHYEGRDGLNLHHYDFYRLDDSGVVAGELAESAQDPKVV
                     TIIEWAETVDFALPKTRMVVNLGHLADENHRQLTLDLPASYQHLNSLVLK"
     gene            4737..6524
                     /locus_tag="VFG56_00635"
     CDS             4737..6524
                     /locus_tag="VFG56_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET6622424.1"
                     /translation="MKLSDKVTEKLLLGGAKLDQDKLETLKKTSVKNKTSLQETALKN
                     EVVSDKQLTEMYAKEIDVPFITIDAKNLKKEVAAALPERIARQYNAVVFQVDKDGTHH
                     IALSDPDDVQAVDFIKKYIGDNFKTYIATSDNILSALDAYRGGVSAELDKVIEVQEEK
                     DPLEETVAEEDVAEDSPIAQTVNLLLEYAIRSDASDIHIEPRDDHISVRYRIDGVLQE
                     ANKLPKNVHAALVSRIKILSNLKIDERRVPQDGRFKITLGSRQYAFRVSTLPITTGEK
                     IVMRILDEGTRALTLEELGYWGKAKASIKEAIVQPHGMILVTGPTGSGKSTSLFSILS
                     MLNDPGVNISTVEDPVEYKVPGANQTQVNPTAGMTFIAGLRALLRQDPNIIMVGEIRD
                     GETANLGVQAALTGHLVFSTLHTNNAATCLPRLLDMGIEPFLIASTVRAVVGQRLVRR
                     LCSKCKESYTPEKTEVDQVARLFGLGDETKLKTVHQLEIQAAEEKIGEDIGKELGSTD
                     KTIVKLWRPKKGGCDNCHGTGFKGRMGIYEVLANTLDIQKLITANATSNAIQDQAIKD
                     DMVTMQMDGLIKALRGLTSIEEILRATRE"
     gene            6532..>6785
                     /locus_tag="VFG56_00640"
     CDS             6532..>6785
                     /locus_tag="VFG56_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6622425.1"
                     /translation="MAQFKYSAQSPKGSVVNGTIEASGRESALEILRNQQLKPISVEE
                     SKKINLLSMQIGGNKVPNKELVIFTRQLSTMVSAGVPIMQA"
BASE COUNT         1750 a         1717 c         1584 g         1734 t
ORIGIN      
        1 gctcatgttg ccaaactacc taccggcgat caagttagca gccttggcgc ttacctatta
       61 caagtctttt cgacgcaatt tacctacgac tggatctact tcctccggct ctacgccatc
      121 atcatggtag ctgccccgct atttatctgg gcagctagaa cacgcaagct ggctatcttt
      181 ctggtcttaa gtgcttcggt ctacgccgcc agcttcctta tggaagagcc tgagggtagc
      241 ttgcagtggc agttcatctt cttcgtggca gctagcgtcg gctacaaatt accgtcgatc
      301 attgattatc taaatgacca cgtcagactt aaaaagtggc tgatgtatgg ctcgatcgct
      361 acaacaatcg caatatttgc agttagttat ttcttcgtac tggcttggcc ggatggcgta
      421 ctgatgagtc gagcccaata cagcgctatc cacgatgtca ccgaagtcgt attctccaat
      481 aaccctttga tgccagctcg ggtagcacta tcattcgcct ggtttgccgg tatgatctca
      541 ttgttccatg tgctgaaacg accgatcgct aagtacttag gctggatgtt tgcccaattc
      601 ggcaattatt cactcagcgt ttactgtctc caagccgtag tcctgatctt cttcaactgg
      661 ctagtgccgt ttacgaccaa cttcttttat aactttttca tcaacctagg gctactgcta
      721 atcatctggt taataatgcg aacgaagttg gccaaaaata ttttgcctcg ttagctatgt
      781 attttacccc ttagacctgt taaaatagct cttgcatttg tcttgtttta tcggggagtg
      841 gcgcagctcg gtagcgcgcg cggtttggga ccgtgaggtc gcaggttcaa atcctgtctc
      901 cccgaccaag aaaatagcct ggcttcagcc gggttatttt cttgggttag atatcgagct
      961 agaagttttc tgctaaaata tctactgcca acgtcggggt gtggcgcagc tcggtagcgc
     1021 gcaccgttcg ggacggtgag gtcgctggtt caaatccagt caccccgacc aaaataagaa
     1081 ttccagcttg tctggagctt ttattttgga tagaggatga tatgaacccg cgacgcatct
     1141 tcagcctcaa gcgaagatgc aagtcgccgg gtcgtaagca aagccgcgcg cagaaattta
     1201 tttcgagccc ggctaccggt tatatcgctt tagcgagtaa tccagtcacc ccgaccaaat
     1261 aaaatcgttc acttatgggg actatttttt tacctattct gctcttcctc ggtttcgacg
     1321 tactggccgt agataaaagc acagtagcgc tctatatagc tgatgcggag cttcaattct
     1381 tggatttctg aaactaagtc aaaggtgcgc ttatctagcg cagcagcttg ctgttgtaga
     1441 taagctacct ttttgaggtc tctttgcaga gaaaagatct gtgaactgag cctagagata
     1501 tcacggtgga gagaaccaac tcccgcctga actgcttttt ggatatcacc cgggctaagc
     1561 tctgccatag cctaattata gcaggcggcc tattctaacc cctccatctg tctaaaatta
     1621 tatgtagata gacaccgcta tcagctgaag tacgtggcag ccgcgatacg tctagccaga
     1681 tacgaatacg caatcgatat ggtaaagaac caaaccacaa acaagaccac acctatacta
     1741 agcttgggcc aaagcttgcc ccgccactcc gtcaatgcag tgtgagctag aacaaagaat
     1801 gcgccggtta cggcaatttg caacaaaaaa gctaaccatg agactggaag caagaggtac
     1861 agtaatttga ctactggcca ggcaaacata agtgcaaaca gactagtcca gaatgtcagt
     1921 ttccggtgac tcagataagc gctaataccg gctatcactc ctatgccaat gagtggataa
     1981 actaaagctc caaggatacc tctttcagta atcagatgcg acagccacag catcgcctgt
     2041 agcgatatac ctagggctac gccagttagt atcagtttct tgcctggatt actaaagaga
     2101 gtgtgcatat tcatagtcag ctacttggcg aattcgatgg ctcgggtttc acgaatgaca
     2161 ctgaccttga tagtacctgg atactgcatg gtcgattcga tcttgttggc gatgtcacgc
     2221 gccagcttga tagccgacag gtcatcaata tctttgggac gaacgataac acgaacttca
     2281 cgacctgcgc tgatagcata agccttgtcg atgccatcga agcttgtagc gacgttctcg
     2341 agctctttca tccgctcgac gaagttctca gccgagatgt tgcgggcacc cggtcgggca
     2401 gcagacagag cgtcgcaaac ccggacgatc agtgcttccg gtgtagtggc ttcgacgtcg
     2461 tcgtggtggg caccgatagc gtgggcgata tcatcagata gaccatattt acgagccagc
     2521 tcttcgccga tatggtggtg cttgccttcc atgcggtggg tgacggcttt accgatatca
     2581 tgcagcaagg tagccatctt tgtgacttga acattggcgc cgatctcttc ggcgattagc
     2641 cccgccatat gagccatttc ggtcgagtgc ttcaatacat tctgaccata cgaagttcta
     2701 aacttaagtt caccaagcag cctcagtaac tccggtggta caccgactac accgacttca
     2761 cgagcggcat cttcaccggc tcgttcgata tccttgtcga tttgcttgtg ggctttggct
     2821 acgacctctt cgatgcgagc cggatggata cgaccgtctt tcatcaacat ctccagggtt
     2881 aacctagcta cttgccgacg aaccgggtcg aagctagaca agacaatcat acccggcgtg
     2941 tcgtcgacca aaatgtcgac accggtggct cgttgcaggg cctggatgtt acggccttct
     3001 ttaccgatga tccgaccctt catctcgtcg ccttctagtt ttatggcggt aactgttcgc
     3061 tcagctgtga cgtcactagc catccgctcc atggcactga ccaggatagt ctgagcccgc
     3121 tcttcggcat cctcttgtgt gtcgcgctgt agcttagaca ccaaaccaag cagatcctgt
     3181 ttgatatcgc gctcggtcat agccatcaat ttttcggtag catctttttt ggtcagcttg
     3241 gcaatctttt ctagcttatc ttgttgcttg gtccggatat cacgaatctc atctttcagg
     3301 tcatcaattt cgtcttcttc cttacggagc ttttcgctac gcttatctag gtcttctaat
     3361 ttcttgtcca aattagtctc acgcgaagct aaacggtcct cggacttttt gatttccttg
     3421 cgacgttctt catcggactt ttgagcatct ttagaaatag ttatggcttc atctttagcc
     3481 ttgaggacga tatcgctagc cttactctta gcatcagcaa ttagcttgtc agccttgtgg
     3541 ccttttttag aggcgcgggc ttgattgaca atggtaactc cgccggcacc aacaagcaag
     3601 ccgacgacgg caaaaatgac ttcgaacata gacgttcctt tctgttactt taaaaggcta
     3661 ggtcaaaaac gatcgctatc acttcactag tttttagata agccttagaa aatcaaataa
     3721 tttaggtaaa ggtaattaaa cggatttagg aaaatcaaca actacctaaa atcaatatta
     3781 cgccaaaaaa aatcgtttgg ccacttaatg ttttggcggt gtgatgcggg cttcagcacc
     3841 gtactcgggt aaaactagcc tgtcgttatg accgtcttta agagcgtgca aacccttttt
     3901 ttgccagtta tttgacgcag cgccgacgat tgggatgtcg agggcataac tcagtgtatt
     3961 catgacggtt atgccgatac gaagcccggt gaaactgccc ggacccttaa agacaactag
     4021 cccggtcaag tcactaaaaa cagcgccctc tcttttgatc atcttagcga tatgggctag
     4081 taagccgtca gctagattgc gatcggccgg ccaatcagct tcagctagcg tttgaccctc
     4141 gcgactaagc aggcgcagct cggccacacc agtagcggtt ttgatagcta ggatcatttg
     4201 aggactaggc tatttaggtg ctggtagctg gctggtagat caagcgtcag ttgacgatgg
     4261 ttctcatcgg ctaagtgacc gaggttgacg accatacggg ttttaggcag ggcgaaatca
     4321 accgtttcgg cccactcgat gatggtaacc acttttggat cttgggcact ttctgccaat
     4381 tcaccggcta ccacacccga atcatcgagt ctatagaagt catagtgatg taaattgaga
     4441 ccgtcgcggc cttcatagtg tcggctcagc gtaaagcttg gactttggac gtcttcgtcg
     4501 atttccaatc cgtcggcaat accgcgcact aaagtagttt tgcctgcgcc tagatcaccg
     4561 actagctcga tgacctcacc gcccctcagg agttttccca acttataacc aaaggcttgc
     4621 atagctacgt cagaggtgat tgctctttcc ataactataa ctatacgaaa aatgtcgaaa
     4681 aaagctaagt caattgtcac taatcgagct taaccgttat actaataagc attaccgtga
     4741 agttatctga caaagtaacc gaaaaacttc tcctgggtgg ggccaagcta gatcaggata
     4801 aattagagac ccttaaaaaa acctctgtca agaacaaaac ttccctgcaa gaaaccgctc
     4861 ttaagaacga ggtagttagc gataagcaac taaccgagat gtatgccaag gaaatcgacg
     4921 taccgtttat caccatagac gctaaaaacc tcaaaaaaga agttgccgcc gccctaccgg
     4981 aacgaatcgc tcgccaatac aacgctgtag tgttccaggt ggataaagac ggcacccacc
     5041 acatcgccct gtctgaccct gacgacgttc aagcggtcga cttcatcaag aagtacatcg
     5101 gtgacaactt caaaacctat atagcgactt cagacaacat cctcagcgcc ctcgacgcct
     5161 atcgcggcgg cgtgtcggca gagctcgata aggttatcga ggttcaggaa gagaaagatc
     5221 cgttggaaga aactgtggct gaagaagacg tggctgaaga ctcgccgatt gctcaaacag
     5281 tcaacctgct actggagtat gccatcagga gtgatgcttc cgatatacac atcgaaccgc
     5341 gagatgatca catatcagtt cgctaccgta tagacggcgt cttacaggag gccaacaagc
     5401 taccaaagaa tgtccatgcc gccctggttt cacgtatcaa gattttgtcg aatctcaaaa
     5461 tcgatgagcg ccgggtgccg caggacggcc gtttcaagat tacactgggc tcacgtcagt
     5521 atgccttccg tgtcagtact ctgccgatta ctactggcga aaagattgtc atgcgtattc
     5581 ttgacgaagg tacgcgggca ttgaccctag aggagttagg ttactggggt aaggccaaag
     5641 ccagcattaa agaggctata gtccagccac acggtatgat tctagttacc ggccctaccg
     5701 gtagtggaaa gtcaaccagt ctattctcaa tcctcagcat gctcaacgat ccaggtgtca
     5761 acatctcgac cgtcgaagac cccgtcgaat acaaagttcc aggcgctaat cagacccagg
     5821 tcaacccgac cgctggtatg acttttatcg caggcttaag ggcattgctt cgccaagatc
     5881 cgaacatcat catggttggt gagattcgtg acggtgagac ggccaattta ggcgtccaag
     5941 cagcgttaac tggtcactta gttttctcta ctctacacac caacaacgcc gccacctgtt
     6001 tgccacgtct actagatatg ggtatcgagc cgtttttgat cgcttcgact gtccgagccg
     6061 tcgtcggtca acgactggtt cgacgattat gctctaagtg caaagaatca tacactccgg
     6121 agaagacaga ggtcgatcag gtagctaggc tatttggtct aggtgacgaa actaagctaa
     6181 aaaccgtcca ccagctagag atccaggctg ctgaagaaaa aatcggtgaa gacatcggca
     6241 aagaactagg ttcgaccgat aagactatcg tcaaactgtg gcggccgaaa aagggtggtt
     6301 gtgacaattg ccatggcacc ggtttcaagg gtcgaatggg tatctatgag gtcttggcca
     6361 acaccctcga cattcaaaaa ttaatcactg ccaacgccac ttccaacgcc atccaggatc
     6421 aagccatcaa agacgatatg gtaaccatgc agatggacgg actaatcaaa gctcttcgcg
     6481 gtctaaccag catcgaagaa attttgaggg ctacccgaga gtaagtaagc tatggcgcag
     6541 tttaaatatt cagctcaatc acccaaaggc agtgtggtaa acggtactat cgaagccagt
     6601 ggccgtgaat cggcgctcga aatcctaaga aaccaacaac tcaagccaat atcagtggag
     6661 gaatccaaaa aaatcaatct attaagcatg cagatcggcg gcaataaggt gcccaacaaa
     6721 gagctggtta tcttcacgcg gcagctgtcg actatggttt cggctggcgt acctatcatg
     6781 caggc
//
LOCUS       DASSBW010000024         6154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1700110, whole genome shotgun sequence.
ACCESSION   DASSBW010000024 DASSBW010000000
VERSION     DASSBW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700110"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFG56_00645"
     CDS             <1..359
                     /locus_tag="VFG56_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622426.1"
                     /translation="TPRGVRTMVVDVFVNHEKIGMNLIEKAVATVPVGTLKVARKPKI
                     SQPEQWGDRLWLISVKAQTPPGREWLIEQYFIDSLVHLDQKRRGPDCLVRQPIARYAD
                     KKAEASFRRAVQAADD"
     gene            complement(377..452)
                     /locus_tag="VFG56_00650"
     tRNA            complement(377..452)
                     /locus_tag="VFG56_00650"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(417..419),aa:Trp,seq:cca)
     gene            563..1201
                     /locus_tag="VFG56_00655"
     CDS             563..1201
                     /locus_tag="VFG56_00655"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6622427.1"
                     /translation="MNALSPQKPLMIVMTGLPGSGKSFFADQFAETFKAPLISADKLR
                     QIVAPSASFSREENQVIHQLTVNQLGQVLKTQKTVIFDGLANGRSERAELSRMAKAAG
                     YRLLIIWVQVDEASALRRSVQPNRRTKHFDSDKTPLTPELFEQFKKRFMPPLKTDDFL
                     VISGKHTYATQAKVVLKKIVEPRSVNPIARPQSGQDGSDVSQNTKTRNIIIR"
     gene            1158..1901
                     /locus_tag="VFG56_00660"
     CDS             1158..1901
                     /locus_tag="VFG56_00660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HET6622428.1"
                     /translation="MFRKTRKPATSSSAKPAGLNDTDFGLITIRRSGLSRSIRLRLLP
                     DGTLTATMPKRTPLYLLKSLIDGSRHELLDQVREVKSKRPNYHDGQSVGRSHTLRIKH
                     IEGAEPRGRLAAQDLLIDLPPTWSEESPEAQSFIRDWYLKALKKEARSYLPRRLQYLA
                     EHFGFNYTKIRFGNPKGRWGSYSSSGTISLNVALMNLPLEVVDYVLVHELCHSRHPHH
                     QIRFWQEVEACLPDYKSLRRQLKEYSPYL"
     gene            1961..3592
                     /locus_tag="VFG56_00665"
     CDS             1961..3592
                     /locus_tag="VFG56_00665"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6622429.1"
                     /translation="MQPRGIDPEKLLFFDKRIVLTIRTTGLIFSLSLAATCLLIYRGA
                     LTIEGAQLWACLPIAASLIGLGLLEAFWNPYTRAKVGFYVLVYMLVGVPLSVLVTGFS
                     TMIFMGWTLLLMVTVIFFGWRRALFGFSLMLTALIIWLVQNSASFSHATVFSYLLSAV
                     AVGILCLLVLSVWQLTTKTIREVENAQKQQNIERNQLLTLINSLNVAVLTVSSNNRIR
                     LYNAALLNLLDTNDSLAGKPLADVLALEDGDGEPFNGSVTDAKLPFERDDLSLRLGDG
                     EVVRLLISGAPIRGASSTKAEGYILLVRDITKAKSLEEERDEFISVISHELRTPVTIA
                     EGTLSNLTLLLDKGATAAKLKPAVKEAHEEIVYLAAMVNDLGTLSRAERGVGDEAEEI
                     ELNNLVNELYHRYQPSAQKKDLKLNLDVDPKLGRLLASRLYLEEVLQNLITNSIKYTL
                     SGSVTICAHKKAGGVEFAIKDTGIGMSKSDQRQIFKKFYRSEDYRTRETSGTGLGLYV
                     THKLASKLHTNIEVSSRLNHGSTFSFHLKSAKKST"
     gene            3673..3915
                     /gene="secE"
                     /locus_tag="VFG56_00670"
     CDS             3673..3915
                     /gene="secE"
                     /locus_tag="VFG56_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET6622430.1"
                     /translation="MAKTKTVKQRKSVVRVIMAPFAGILNYIKGSWYEIRQVRWPDRK
                     ATWSLTLAVILFSGFFAGLIVLLDYIFHWLFKEILL"
     gene            3912..4460
                     /gene="nusG"
                     /locus_tag="VFG56_00675"
     CDS             3912..4460
                     /gene="nusG"
                     /locus_tag="VFG56_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET6622431.1"
                     /translation="MSSNKRYDSSRAWYAVHTYSGYEEKVAESIGQRVESVDMADKIF
                     DVLVPKEKQIEIKNGKRKIVEKKIFQGYVLVEMKMTEDAWYIIRNTPGVTGFVGSGTD
                     PTPVSNAEITKIKKRMGVEEPKHHINFSEGEVVNIVDGPFKGFDGSISEIDTQKGKIK
                     VMVSMFGRETPVELDALQVKKV"
     gene            4510..4878
                     /locus_tag="VFG56_00680"
     CDS             4510..4878
                     /locus_tag="VFG56_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622432.1"
                     /translation="MNQPAPRIYAVIHHGDRIFLPESGMPSCQAELGMTYERQIGSHI
                     LQSYRLGVSNQCSLGYYDDSLGYLMRLSHGALMPEGDDWLSGQTAAEYLMAGKDVAAA
                     YFAKQALGRFSASRRLDIVG"
     gene            4959..5384
                     /gene="rplK"
                     /locus_tag="VFG56_00685"
     CDS             4959..5384
                     /gene="rplK"
                     /locus_tag="VFG56_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET6622433.1"
                     /translation="MAKQVIGNLKLRVPGGKASAGPPVGSTLGQWGLNMMDFINPFND
                     ATKEYNGKDVIVHLKIYEDRTFDWVCLGQPVDDLIREKIGIKKGSGKPHSEKVGKISR
                     AQLEEIAQTKMSVLNANDLDGAVKVVAGTARSMGVEVEQ"
     gene            5451..5969
                     /locus_tag="VFG56_00690"
     CDS             5451..5969
                     /locus_tag="VFG56_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET6622434.1"
                     /translation="MVIYTIGHSTHPIDEFIDMLASFGVKQLVDIRTLPGSTRQPQFN
                     QEDLGPSLKAVDIDYVYLKKLGGLRKKTSDSVNLGWRNLSFRNYADYMQTDDFKSALG
                     RLTELAEDKVTAIMCAEAVPWRCHRSLVADALQVRGIEVSHIMTKNKANPARLTPFAK
                     VSGQNITYPSEA"
BASE COUNT         1647 a         1508 c         1467 g         1532 t
ORIGIN      
        1 tcacgccacg gggtgttcgc accatggtag tcgacgtctt cgttaaccat gagaagatcg
       61 gcatgaatct gattgaaaaa gccgttgcta ccgttcctgt gggtacacta aaggttgcca
      121 gaaaaccaaa aatcagccaa ccagaacagt ggggtgatcg attgtggctg atcagcgtca
      181 aagcccagac tccaccaggc cgggagtggc tgatcgaaca gtactttatc gattcgttag
      241 ttcaccttga ccaaaaacga cgcggcccag actgtttagt tcgccagccg atagcccgtt
      301 atgccgacaa gaaagccgag gcaagcttcc gccgtgccgt tcaagcggct gacgattagg
      361 cttaatttag tacttttggc aggagtgata ggactcgaac ctacaaccaa cggttttgga
      421 gaccgctact ctaccaattg agctacactc ctatcaaaag gactaaatcc attgtcgccg
      481 ctaaattata gcaaatacca gcgttaatct aaaagctaac cacaacacaa tttgcttaaa
      541 tttgttatac taagccgtaa gtatgaatgc gctcagtcct cagaagccgc tgatgattgt
      601 tatgaccggt ctgcctggat cgggcaagag ttttttcgct gatcaatttg ctgagacctt
      661 caaggcacca ctaatcagcg ccgataaact acgccaaatc gtagcaccaa gcgccagctt
      721 cagtcgcgaa gagaaccaag tcattcacca actaaccgtc aatcaacttg gtcaggttct
      781 aaaaacccaa aagaccgtaa tcttcgatgg tctggccaac ggccggtctg agcgagctga
      841 gctaagtcgt atggccaaag ccgctggcta tcgcttattg atcatctggg tacaggtcga
      901 cgaagcttcg gctttgcggc gttccgtcca gcccaatcgc cggaccaaac acttcgactc
      961 cgataaaaca ccgctaactc ctgagctctt cgagcaattc aaaaaacgtt ttatgccacc
     1021 gctaaagact gatgacttct tggttatcag cggtaagcat acttacgcta ctcaggctaa
     1081 agtagtactt aagaagattg tcgagccgcg ttcagtcaac ccaattgccc gacctcagtc
     1141 gggccaggat ggttccgatg tttcgcaaaa cacgaaaacc cgcaacatca tcatccgcta
     1201 aaccggccgg tctcaacgac acggattttg gcttgatcac catccgtcgc agcggactga
     1261 gtcgatctat ccgtctacgc ttactaccag acggtacctt aaccgccacc atgccaaagc
     1321 ggacgccgct ttacctgctt aagtcactga tagatggttc gcggcacgaa ctactcgatc
     1381 aagtccgtga ggttaagtcc aagcgaccaa attatcatga tggtcagtct gttggtcgct
     1441 cgcatacgtt gcggattaag catatcgaag gagcagagcc tcgtggtcga ctggcggccc
     1501 aagacctgtt gatcgacttg ccacccacat ggtcagaaga atcacctgag gcacaaagct
     1561 ttatccgcga ctggtatctc aaggcactca agaaagaggc tagaagctac ctgcctcgtc
     1621 gactacaata tctagccgag cacttcggtt ttaattatac gaaaatcagg tttggtaacc
     1681 cgaaaggccg atggggcagt tactcctcga gcggtacgat cagcctcaat gtagccctta
     1741 tgaatttacc actcgaagtt gtcgattatg tcttggtcca cgaactttgt catagccgtc
     1801 acccgcacca ccaaatacga ttctggcaag aggtggaggc ttgtttgccg gattacaaat
     1861 ctttaaggcg acaactgaag gaatacagcc cttatttgtg aggctaagcc gcctatgttt
     1921 ataatactgg tatgaacaaa cacttgggtg ggctaaaatc atgcagccac gcggcataga
     1981 tcctgaaaag ctgctgtttt ttgataagcg aatcgtccta actatcagaa ctactggttt
     2041 gattttttcg ctgtcgctcg cggccacctg tctgcttatc taccgtggcg ccctaacgat
     2101 tgagggcgct cagctgtggg catgcctgcc gattgcagcc agccttatcg gtctcggtct
     2161 gcttgaagct ttttggaatc catatacacg agccaaggtg gggttttatg tattggttta
     2221 tatgctagtc ggcgtaccgc tgagtgtgct ggtgactgga ttttctacca tgatattcat
     2281 gggctggaca ttactcctga tggtgacggt tatcttcttc ggttggcgac gggctctatt
     2341 tggcttcagc ctaatgctta cagccttaat catctggctg gttcaaaatt cggctagttt
     2401 tagccatgcc actgtatttt cctacctcct gtcggccgta gcggtcggca tcctgtgtct
     2461 gttggtcctg agtgtctggc aattgaccac taagactatt cgcgaagtag agaatgcgca
     2521 gaaacaacaa aatattgagc gcaatcaact acttactctg atcaacagtc tcaatgtcgc
     2581 cgttctaaca gtaagctcca ataaccgtat aaggttgtat aacgccgctc ttttgaactt
     2641 gctcgatacc aatgattcat tggcgggtaa accattggcc gatgtcctag ccttggaaga
     2701 tggtgacggt gagccattta acggttcggt aaccgatgct aagctgccgt ttgagcggga
     2761 cgatttgtcg ctgcgactag gtgacggaga agttgtacgg ttgctaataa gcggcgctcc
     2821 gattcgtggg gctagtagta ctaaagccga gggatacatc ttattagtgc gcgatatcac
     2881 caaagcaaaa agcctagaag aggaaagaga cgagttcatc tcagtgatta gccacgagct
     2941 gcgcacaccg gtgaccatcg ccgagggtac gctatccaac ttaactctgc tcttagataa
     3001 aggtgccacc gccgctaaac ttaagccggc cgttaaagag gctcacgaag aaatcgttta
     3061 tctagccgcc atggtaaatg acctaggcac gctatcacga gccgaacggg gtgttggtga
     3121 tgaggccgaa gaaatcgaac ttaacaattt ggtcaatgaa ctataccacc gctatcagcc
     3181 aagtgctcag aaaaaggact tgaaactgaa ccttgacgtc gaccctaaac ttggccgcct
     3241 tttagctagc cgtctatacc tcgaagaagt cctgcaaaac ttgatcacta actcaatcaa
     3301 gtacacccta tcgggtagtg ttacgatttg cgctcacaaa aaagccggtg gagtggagtt
     3361 cgccataaag gacactggca ttggtatgtc caagtctgat cagcgtcaga tatttaagaa
     3421 gttctatcgc tcggaagact accgcactcg cgaaacctct ggcaccggct taggtctcta
     3481 cgtcacccat aaactagcca gtaagctaca caccaacatt gaagtcagca gtcggttgaa
     3541 tcacggctca accttcagct ttcacctgaa gtcagccaag aaatcaactt gaccaaggtc
     3601 atagtcctca gttataatct tgtttgacaa gtcctaggag aaacctaggc tttttaaata
     3661 ggagactcat ggatggccaa gaccaaaacg gttaagcagc gaaagtcggt tgtgagagtt
     3721 atcatggcgc ctttcgccgg tatcttaaat tacatcaaag ggtcttggta tgaaatccgc
     3781 caagttcgtt ggccggatcg caaggctaca tggtctctta ctctggcggt tatccttttt
     3841 tccggcttct ttgccggact tatcgtgctg ctggactata tttttcactg gttgtttaag
     3901 gagatattac tatgagttca aacaaacgtt acgattcatc gagggcttgg tacgccgtcc
     3961 atacttactc aggttatgaa gagaaggtgg cggaatcgat tggtcagcgc gtcgaatcgg
     4021 ttgatatggc cgacaagatt ttcgacgtat tagtacccaa ggaaaaacaa atcgagatca
     4081 aaaacggcaa acgtaaaatc gttgaaaaga aaatctttca gggatacgtc ttagtagaga
     4141 tgaagatgac cgaagatgcc tggtacataa tccgtaacac ccctggtgtg actggttttg
     4201 ttggttcagg cactgatcca acgccggtat ctaacgctga gatcaccaag atcaagaagc
     4261 ggatgggcgt cgaagagcct aagcaccaca tcaatttttc agaaggtgaa gtggttaaca
     4321 tcgtcgatgg tccgttcaag ggcttcgatg gttcgattag tgaaatagac acccaaaaag
     4381 gtaagatcaa agtcatggtc agtatgtttg ggcgtgaaac gccggtggag ctcgacgcct
     4441 tgcaggttaa gaaagtctaa tcaaacaaat accccggcag accggggtat ttttataagg
     4501 gagttagtta tgaaccagcc agcacctcgc atctacgccg tcatccacca tggtgaccga
     4561 attttcttac cagagagtgg gatgccatca tgccaggctg agctcggtat gacttatgag
     4621 agacaaatcg gcagtcacat cctacaatct tatcgtctag gtgttagcaa tcaatgcagc
     4681 ttaggttatt acgacgatag cctaggctat ttgatgcggc tatcgcacgg cgcacttatg
     4741 cccgaaggcg acgactggtt gtctggtcaa acggcagctg agtacctaat ggccggcaaa
     4801 gacgtagcgg ctgcttattt tgccaagcag gctttaggta gattttcagc cagccgccgc
     4861 ctagacattg tcggttaatt ttgctacaat ttgtcactgt tacgcactgc aaacgcttca
     4921 actaatgcag taaatctaat tcataaggaa attacataat ggccaaacaa gttattggta
     4981 atctaaaact tcgcgtgccg ggcggcaaag ctagtgccgg tcctccggtt ggctcaaccc
     5041 ttggtcagtg gggtcttaac atgatggact tcatcaaccc tttcaacgac gctaccaagg
     5101 aatacaacgg caaagacgtc atcgttcacc tcaagatcta tgaagaccga acttttgact
     5161 gggtctgcct cggtcaaccg gttgacgacc taatccgcga aaaaatcggc atcaaaaagg
     5221 gttccggcaa accgcacagt gaaaaggttg gcaagatttc tcgcgcccag cttgaggaaa
     5281 tcgcccaaac caagatgtcg gtcctaaacg ctaacgatct tgacggtgct gtcaaagtcg
     5341 tagccggtac cgctcgctct atgggtgtcg aagtcgaaca gtaagctcat tttgacggct
     5401 cttaagcacc actcgttttc gggtggtgct ttaggtttta taattgaagt atggttatct
     5461 acactatcgg ccactcgacc catccgatcg atgaatttat cgacatgcta gccagctttg
     5521 gcgtcaagca gcttgtcgat atccggactt taccgggctc gacgcggcaa ccacagttta
     5581 atcaagagga tttaggccct agccttaagg cagtcgacat tgactatgtc tatttaaaaa
     5641 agcttggcgg cctgcgcaag aagacttcgg attcggtcaa tcttggatgg cgcaatctgt
     5701 cgtttcgtaa ttacgctgat tatatgcaaa ctgatgattt caaatctgcc ctaggccggt
     5761 taactgaact agccgaagac aaagtaaccg ctatcatgtg cgccgaagcc gtgccatggc
     5821 gttgccatcg tagcttagta gccgatgcct tacaggttcg tggcatcgaa gtcagtcaca
     5881 tcatgactaa aaataaagct aatccggcta ggctaacgcc attcgccaaa gtctccggcc
     5941 aaaacatcac ttatccgagt gaagcttgac ctataagggg ctcaagccag ctataatggt
     6001 aagtaatgga tcaaactcta gctttagaag tactgattga gggcgagaat acgctactga
     6061 ccgggccagc cggttcaggc aagacctatg tcttaaatca attcattcgt tacgccaaat
     6121 cagtcggtaa gaatatttca gttacagcca ccac
//
LOCUS       DASSBW010000025         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1328672, whole genome shotgun sequence.
ACCESSION   DASSBW010000025 DASSBW010000000
VERSION     DASSBW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328672"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /locus_tag="VFG56_00695"
     CDS             complement(<1..1762)
                     /locus_tag="VFG56_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6622435.1"
                     /translation="MSDNFREFNQHLTDNARNSLGHADMIARSLGSAYIGTEHLLLGV
                     LSQEASVGAKLLADAGITLDRAKLALNLTPRALVVSTGQKGLSETAKLTLRMSWEIAQ
                     EFHQDYCGTEHILFSILSQKNARATVLLKDMNVPIDELLGEIEGYLNRQQSDFEDGTF
                     ARTKVPHRSKQPALNTYGTDLTSKARAGELDPVIGRDKQIARMITILSRRTKNNPILI
                     GEPGVGKTAIVEGLAQRIASEDVPDHLLDKRIIQLDLSAMIAGTKYRGEFEERLKKII
                     DELTEDDKVIAFIDELHLLVGAGAAEGSLDAANMLKPALARGSLHMIGATTLDEFRKH
                     IEKDAALERRFQPIIVPEPTLKDVKAILQGLKTHYEQFHRVKISDEVIDDAIYLSDRY
                     VSERHMPDKAIDVIDEAASLVKVRNNKTPNKARTYQRELNNLNEKMEEAVTSEDYERA
                     ALYKMRISRINEKIDEIRNAHRGPAITMKPDDIAEAVAVMTNIPLSTLKRSEISLLKN
                     LEKHIGKSIIGQHEAVAKVSRSIRRSRSGVASGSRPIGSFIFMGPTGVGKTELARVLA
                     HEVFGSDDALIKIDMSEFSER"
     gene            complement(1860..3011)
                     /gene="nusA"
                     /locus_tag="VFG56_00700"
     CDS             complement(1860..3011)
                     /gene="nusA"
                     /locus_tag="VFG56_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET6622436.1"
                     /translation="MDTINVKQLVTAVGTIAEEKNLPEETVLDVVEQAVAAAWRRDNG
                     ERDQEVRAELNTNDGSAKVFVVHHVVEEVENPASELTLEEAQKLKKDAKVGDVIEDEH
                     QATSFGRVAAQTAKQVVLQKLREAEREVVMVEFADKIGTVLTGTVQRVEPRLVRVELG
                     KAIGILPASEQIPGEFYSIGSRIKVFLKDVERENRGPQLILSRANEAFIEYLFSQEVP
                     EMETGAVEIKAIAREAGRRTKLAVASTVPGVDPVGTFVGGHGTRVTAVMNEIGDQEKI
                     DIISYDEDSKNFIANALSPAEISSIVLDDKNQRADVIVSEDQQSIAIGRSGQNVRLAS
                     RLTGYELDVKTAEAPVTDAKPEATKSAKKKTSKNIEDDLLSALNEQSEK"
     gene            3131..>3846
                     /locus_tag="VFG56_00705"
     CDS             3131..>3846
                     /locus_tag="VFG56_00705"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622437.1"
                     /translation="MRTVERLYETFKPNHYKVSFDLSLPKHQFAGRVKITGKASGGQV
                     KLHAKQLNLKNPLLNGQVAEVESADDDVVLLSAAGFEGGEATVEIDFTGQISDAMHGI
                     YPCYFDLNGQKQELIATQFESHHAREAFPCVDEPEAKASFDVQLTTKPGLQVLGNMPQ
                     AGQSTLDDGNWKTTFETTPVMSSYLLAFVVGDLQSKSAKTKDGVTVTTWATRAQSADS
                     LDFGLDVAVRSIEFFNDYFGV"
BASE COUNT          901 a          994 c          960 g          991 t
ORIGIN      
        1 gtcgttctga gaactcactc atatcaatct tgataagagc atcgtccgaa ccaaagactt
       61 catgggccag tacccgggca agttcggtct taccgacgcc agtcggcccc atgaagataa
      121 acgagccgat aggccttgag ccgctagcca cgccggagcg cgagcgacgg attgaccggg
      181 acaccttcgc aaccgcctcg tgctgaccga tgatagattt accgatatgt ttttcgaggt
      241 tcttaagtag gctgatttcg gagcgcttca gggtgctcaa aggaatgttg gtcatcacgg
      301 ctaccgcctc agcgatatcg tctggcttca tagtgatggc cggaccgcga tgagcgttac
      361 ggatttcatc aatcttttcg ttaatgcgag agatccgcat cttataaagg gccgcgcgtt
      421 cgtaatcctc cgaggtgacg gcctcttcca tcttctcatt gagattattg agctctcgtt
      481 gataagtccg ggctttgttg ggcgtcttat tgttgcgaac cttgaccagt gaagcggctt
      541 cgtcgataac gtcgatagcc ttatctggca tgtgccgctc ggatacgtaa cgatcgctta
      601 ggtagatagc gtcatcgata acctcgtcgc tgatcttgac tcggtgaaat tgctcatagt
      661 gagtttttag accctgcagt atagctttga catctttgag tgtcggttcc ggcacaatga
      721 tcggttggaa gcgacgctcg agggcggcat ctttttcgat atgcttacgg aactcgtcca
      781 gggtagtagc gccaatcata tgtaggctgc cacgagccaa agccggtttg agcatattgg
      841 cagcgtcaag cgaaccttcg gccgcaccag ccccaaccag caagtgaagt tcgtcgataa
      901 aagctatcac cttgtcgtct tcggtaagct cgtcgattat cttcttgagt cgttcttcaa
      961 actcaccgcg gtatttggtg ccggcaatca tggccgacaa atcaagttga atgattcgct
     1021 tgtcgagcaa gtgatcgggc acatcttcac tggcgattcg ctgggctaga ccttcgacta
     1081 tagccgtctt accgacaccc ggttcaccaa tcaaaatcgg attgttctta gtgcgacgag
     1141 acaagatcgt gatcatgcgt gcgatctgct tatctcgtcc gataaccgga tcaagttcgc
     1201 cagcccgggc tttggacgtc agatcggtgc cgtaagtatt gagcgccggt tgcttggacc
     1261 gatgaggtac tttagttcga gcgaatgtac cgtcttcaaa atcagactgc tggcgattga
     1321 gataaccttc gatctcaccg agcaactcat cgatcggtac gttcatgtct tttaatagca
     1381 ctgtggcccg ggcgtttttc tggctaagga tgctaaatag gatatgctct gtaccgcagt
     1441 agtcttgatg gaattcctga gcaatctccc agctcatcct cagcgtcagc ttggcagttt
     1501 cggacaagcc cttctgaccg gtcgagacaa ctaaggcccg gggtgtcaaa ttgagagcta
     1561 gtttggcacg atccagagtg atgccggcat cagccagcaa cttggctcca accgaagctt
     1621 cttgactgag gacgccaagc aataagtgtt cagtgccgat ataagcgctg ccaagacttc
     1681 gcgcaatcat gtcggcgtgg ccgagcgaat tgcgcgcgtt atcagtcaaa tgctggttaa
     1741 attctctaaa attatctgac ataaattccc tctcttattg tgagcaatca ctcccaataa
     1801 ctcaagtata taaaattagc actcttatcg caagagtgcg cattccccag tggttgagac
     1861 tatttttctg actgttcgtt tagagcactc agcaggtcgt cttcgatatt tttgctggtc
     1921 tttttcttgg ccgacttagt ggcttccggc ttggcatctg tgacgggagc ttcggcggtc
     1981 ttgacgtcaa gctcatagcc ggtcaaacgc gaagcaagac ggacgttctg gccactacgg
     2041 ccgatagcga tggattgctg atcttcgctg acgatgacat cggcccgctg gttcttgtca
     2101 tcaagcacga tcgaactgat ttcggctggg ctcagggcgt tagcgataaa gttcttggaa
     2161 tcctcatcgt aggagatgat gtcgatcttc tcttgatcgc ctatctcatt cataacagca
     2221 gttacacgag taccgtgacc gccaacgaag gtgccgaccg gatcgacacc aggaacggtt
     2281 gaagccacgg ctagcttagt ccggcgacca gcttcacgag cgatggcttt gatttcaact
     2341 gctccggttt ccatctccgg aacttcctga gaaaacagat attcaataaa ggcctcgttg
     2401 gcccgagaaa gaatcaattg tggtccgcgg ttctcgcgtt cgacgtcttt taagaaaact
     2461 ttgatacgag aaccgatcga gtaaaactcg cctgggatct gctcgctggc cggcaagatg
     2521 ccgatggcct tgcccagttc aactcgtact aatcgtggct caacccgctg gacggtaccg
     2581 gtgaggacgg tgccgatttt gtcagcaaac tcaaccatga caacttcgcg ctcggcttca
     2641 cgcaactttt gcagcactac ttgcttggcg gtttgggcgg ctacgcgacc aaagctggtc
     2701 gcttggtgct catcttcgat aacatcaccg actttggcgt cttttttgag tttttgagct
     2761 tcctccagtg tcagctccga agccgggttc tcgacttctt cgacgacatg atgaacgaca
     2821 aagaccttgg cgctaccatc attggtattg agctcagccc gaacctcttg atcacgctcg
     2881 ccattgtcac gccgccatgc agcagctaca gcttgctcga ctacgtcaag taccgtctct
     2941 tctggaagat tcttctcttc agcgatagta ccaacggcgg taaccaactg ttttacgtta
     3001 atggtatcca tatgaaatct cctttttcta tggtcggtta aatgaaaaat ccgacctgcc
     3061 ggccggaaat gtacatacat ataatatact gtctcagccc taaaaagcaa gctttgctaa
     3121 actaggatgt atgcgcacag ttgaacgatt atacgaaact ttcaaaccaa atcactataa
     3181 ggtgagcttt gatctcagtc tgccaaaaca tcaatttgct ggacgagtca agataaccgg
     3241 taaagccagt ggtggccagg tcaaactcca cgccaagcag ctgaacctca agaaccccct
     3301 actaaacggt caggttgccg aagtcgaatc ggctgacgac gacgtggtgc tacttagtgc
     3361 cgctgggttt gaaggcggcg aagcgacagt ggaaatcgat ttcactggtc agatcagcga
     3421 cgctatgcat ggcatatacc cttgttattt tgatttgaac ggtcaaaaac aagagttgat
     3481 cgccactcag ttcgaaagcc accatgcccg cgaagctttt ccgtgcgtcg acgaaccgga
     3541 agccaaagcc agctttgatg tccaactcac tactaagcca ggcttacagg ttctcggcaa
     3601 catgcctcaa gccggacaat cgacgctaga cgatggaaat tggaaaacga cattcgagac
     3661 gacaccggtg atgagttctt atctgctagc tttcgtagtc ggtgacctgc aatccaagtc
     3721 ggccaagacc aaggatggtg tcactgtgac cacctgggct accagggccc agtcggccga
     3781 cagcttggac tttggtttag atgtggccgt tcgctccatc gaatttttca atgattactt
     3841 tggcgt
//
LOCUS       DASSBW010000026         5481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1652935, whole genome shotgun sequence.
ACCESSION   DASSBW010000026 DASSBW010000000
VERSION     DASSBW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652935"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFG56_00710"
     CDS             complement(<1..451)
                     /locus_tag="VFG56_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6622438.1"
                     /translation="MQILSELNFVLSVIIGLLILVALVVVHELGHAIAARRNGVVVEE
                     FGIGFPPRAWGKKLKNGILFSLNWLPLGGFVKLQGEHDAAEKPGDYGAASFWVKTKIL
                     LAGVAINWLVAAVLFMALSWFGLPKILDNQFTVPADTRTDHSPVTVAA"
     gene            complement(458..1165)
                     /gene="uppS"
                     /locus_tag="VFG56_00715"
     CDS             complement(458..1165)
                     /gene="uppS"
                     /locus_tag="VFG56_00715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET6622439.1"
                     /translation="MSSPGKVASDVPNHIGFIVDGNRRWAKSHGLPSYEGHLAGYEAL
                     KDVVLAAADAGVKYISLYTFSTENWSRAEEEVAGLMKLMMRLFKTDLHILTDNDLKLE
                     VLGSRQGLPDKMNQAIDQAEATTASGQRSTVAVCFNYSGQNEITAAVQALMEQAIEPE
                     AIDTEAIRANLYKPNVPDCDLIVRTSGEQRLSNFMMWRSAYSELIFLDKFWPDMRPKD
                     VADIIDEYKRRQRRYGK"
     gene            complement(1165..1719)
                     /gene="frr"
                     /locus_tag="VFG56_00720"
     CDS             complement(1165..1719)
                     /gene="frr"
                     /locus_tag="VFG56_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET6622440.1"
                     /translation="MFDTKPYETKMSGAVDHYQNELKKIRTGRAHPDMLDGILVEAYG
                     AKVPLNQVANITVPEPQLLQVNPFDPSNVKAVVTAIREDQGLGFNPSDDGKIVRVPVP
                     PLTEERRHQIVKQLGEKTEDARIALRATRQAAFKEAKRKKDSKELSENDVARVEKEID
                     KLVSDFNAQLDEIAKTKEQEILTV"
     gene            complement(1832..2428)
                     /locus_tag="VFG56_00725"
     CDS             complement(1832..2428)
                     /locus_tag="VFG56_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET6622441.1"
                     /translation="MAVSLEDVKKLKELTGVGLSDAKAALEEAKGDFDKALQAMRKKG
                     LTKAEKKGDREAREGLVEAYVHSGRIGALVEVNCETDFVARTEDFKELVHDVAMHVAA
                     SNPVYITEADVPAEVRDSKAKEFKDKVISEGKPADMADKIVEGQLKKYFSEQTLLQQP
                     FIKNPDQTVGDLVKQSIAKLGENIVVRQISRVELGVSE"
     gene            complement(2428..3135)
                     /gene="rpsB"
                     /locus_tag="VFG56_00730"
     CDS             complement(2428..3135)
                     /gene="rpsB"
                     /locus_tag="VFG56_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET6622442.1"
                     /translation="MPQVDIKALLEAGVHFGHKTSRWHPKMAPYIHSKRQDSHIIDLT
                     KTVEGLDGALPFLTDVVKSGKQVLLVGTKRQAKDMVKVAAEAVNQPYVAERWLGGMLT
                     NVTTVTDRVKHLKLLEKRMDSGELENRYSKLEVQRFQEEIDDLNKKYGGIKNLNGKPG
                     AVFVTDVIEDANAVREAKKLNIPVVAIVDTNADPTMVDHIIPANDDAIKGLQLIVDYV
                     KAAIEEGLAKQDKGEDK"
     gene            3328..3504
                     /locus_tag="VFG56_00735"
     CDS             3328..3504
                     /locus_tag="VFG56_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622443.1"
                     /translation="MNWSERLVLAFHAVLILGLLLAIGSLTADLAVLLSISLIIAIII
                     FRPVLKVSRLFQRT"
     gene            3664..4044
                     /locus_tag="VFG56_00740"
     CDS             3664..4044
                     /locus_tag="VFG56_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET6622444.1"
                     /translation="MKKDLHPTEYRPVVFQDDSAGFAFLTQSTATTTDTIKWEDGNEY
                     PLVKTHISSASHPYFTGEEKIIDTEGRVDRFKAKFAAAEARKDQLANKAKKNAKRAEA
                     KAQSAKTGQNEAATTNKAGDDTKK"
     gene            4124..5194
                     /gene="prfA"
                     /locus_tag="VFG56_00745"
     CDS             4124..5194
                     /gene="prfA"
                     /locus_tag="VFG56_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET6622445.1"
                     /translation="MPKINLDLNALKDEKHQLEGLLARPDAFAHSDFSQKSKRLSELS
                     ELIDLAIGRDKLIAELDETRQLAGGNDELADLAKAEVPELETKIEAVDEKLFIMLTPK
                     DPNDGKDVIVEIRAGAGGDEASLFAAELLRMYLRYCETHGLKTELMSESSSDAGGYKE
                     VVFSVVGDQPYAQLKFESGVHRVQRVPATESQGRIHTSTVTVAVIPEAEEADIDINPN
                     DLRIDIFRASGHGGQSVNTTDSAVRITHLPSGLVVSNQDEKSQIKNKEKAMKVLRSRL
                     LQQKMDEEAAKAAEHRRSLIGSGDRSEKIRTYNFPQDRITDHRIKYSRSNIPAALDGS
                     IDDLIDHLAAYERELKAQGANA"
BASE COUNT         1309 a         1471 c         1340 g         1361 t
ORIGIN      
        1 ctgccgcgac cgtcaccggg ctgtggtcag tccgggtgtc ggccggtacg gtgaattgat
       61 tgtccagtat cttcggcaga ccaaaccaac taagcgccat aaacaacaca gctgctacca
      121 gccagttgat cgctacgccg gctagtagaa tcttggtctt gacccaaaag ctggccgcac
      181 cgtagtcacc tggcttttcg gcagcatcgt gctcgccctg aagcttgaca aagccaccta
      241 gcggcagcca gttcaaacta aacaaaatac cgttttttaa cttcttgccc catgcacgcg
      301 gcggaaaacc gataccgaac tcttcgacca ccactccgtt acggcgagcg gcgatggcgt
      361 gccccagttc gtggactacc actaaggcta ctaagataag caggccgatg atgaccgata
      421 ggacaaaatt taattcagac agtatttgca ttggccttta tttcccataa cgccgttggc
      481 gccttttgta ctcgtcaatt atatcagcca cgtctttggg tcgcatatcc ggccagaatt
      541 tatctaggaa gataagttca gaataagcgc tgcgccacat catgaagttg ctgagacgct
      601 gctctccact ggtacggacg atcagatcac agtccggcac attcggttta tagagattgg
      661 ctcggatggc ttcagtatca atagcttctg gctcgattgc ctgctccata agcgcctgaa
      721 cggctgcggt aatttcattt tgaccggagt agttaaagca gactgccaca gttgagcgct
      781 ggccgctagc cgttgttgct tcggcttgat ctatagcttg gttcatctta tctggcaggc
      841 cttgacgtga accgagcacc tccagcttaa ggtcgttgtc ggtcaagata tgtaagtcgg
      901 tcttaaacag tcgcatcatc aacttcatca ggccggcaac ctcttcttca gcccggctcc
      961 aattctcagt cgagaaagta tagagtgaaa tatatttaac accggcatcc gccgcagcta
     1021 aaaccacgtc cttcagagct tcataaccgg ctaggtggcc ctcgtagctc ggtagaccgt
     1081 gcgacttagc ccagcggcga ttgccatcga caataaaacc gatgtggttc gggacatcgc
     1141 ttgcgacttt cccgggactc gacatcaaac ggttagtatt tcttgctctt tagtcttggc
     1201 gatctcgtca agttgagcat taaaatcact caccagcttg tcgatctctt tttcgactct
     1261 agccacgtca ttttcactaa gttctttgga gtctttttta cgcttagctt ccttgaaagc
     1321 agcctgacga gtggccctga gtgcaatccg agcgtcttcg gtcttttcgc ccagctgctt
     1381 gactatctga tgtcgccgtt cttcggttaa cggtggcact ggcactcgga cgatcttgcc
     1441 gtcatccgaa gggttaaagc ctaggccctg atcttcacgt atggcagtaa cgaccgcttt
     1501 gacgttagac ggatcgaacg ggttaacttg cagtaactgc ggttctggca cggtgatatt
     1561 ggcgacttgg ttaagcggta ctttcgcgcc atatgcttcg actaagatgc catctagcat
     1621 atcagggtgt gcccgaccgg ttcggatttt ctttagttca ttctgatagt ggtcaacggc
     1681 accgctcatc ttggtctcat atggtttggt atcgaacata ttcctcgctt tctacttcaa
     1741 gaataatttt agcatcgatg gtgaactttg gtctgaaaag taaacgggcc gccggctttg
     1801 tggcagcccg tttattgtag gaataactaa attattcgct aacgcctagt tcgactcgcg
     1861 aaatctggcg aacaacaatg ttctcgccaa gcttagcgat cgattgtttg accaagtcgc
     1921 caacggtctg gtcggggttt ttgataaacg gctgctgcaa aagggtctgc tcagaaaagt
     1981 acttcttgag ctgtccctcg acgatcttat cggccatgtc ggcaggctta ccttcgctta
     2041 taaccttgtc tttgaattct ttggccttgc tatcgcgaac ttcggccggc acgtcggcct
     2101 cagtaatgta gaccgggttg gaagccgcca cgtgcatagc tacgtcatgg actaattctt
     2161 tgaagtcttc ggtccgcgcg acaaaatcag tctcacagtt aacttcaacc aacgcaccga
     2221 tccgaccgga gtggacatac gcttcaacca agccttcacg agcttctcga tcaccctttt
     2281 tctcagcctt ggtcagacct tttttgcgca ttgcctggag ggctttgtcg aagtcgccct
     2341 tagcttcttc tagggcagct ttggcgtcag ataatccaac accagtcagc tcttttaatt
     2401 tcttgacgtc ttccagtgat actgccatta tttgtcctcc cctttatcct gtttggccag
     2461 accttcttcg atagcggcct taacataatc aacaatcagt tgtagaccct tgatggcgtc
     2521 gtcgttagct ggaattatat gatcaaccat agtcgggtcg gcgttggtat cgacgatggc
     2581 aacgaccgga atattcaact tcttagcttc ccggacggcg ttggcgtctt cgataacatc
     2641 agttacaaaa accgcacccg gcttaccatt taggttcttg ataccaccgt attttttatt
     2701 gaggtcatca atttcctctt ggaagcgttg gacttccagt ttggagtaac ggttctcgag
     2761 ctcaccagaa tccattcgct tctctaacaa cttgaggtgc ttgacccgat cggtcaccgt
     2821 agtgacattg gtcagcatgc cgcccagcca acgctcggct acgtacggct ggttgaccgc
     2881 ttcggcagct accttaacca tgtctttggc ttggcgcttg gtgccaacca gcaagacctg
     2941 cttgccggat ttgacaacgt cggtcaggaa cggcaaagcc ccgtctaacc cttcgacggt
     3001 cttagtcagg tcgatgatgt gcgagtcctg acgctttgag tgaatatatg gcgccatctt
     3061 tgggtgccaa cgacttgttt tgtgtccaaa atggacaccg gcttctaata aagccttgat
     3121 atctacctga ggcatctatc cttctccctt cacccggcgc tcgcttcttt taggataggc
     3181 gctgggctgc ttcgttaaaa tattgttacc cggtagattg tagcagctgc ccctgttatt
     3241 gtccagacct tttgacaatc gacgcttgta ggcgtatacc gtaatgagtc gtccgttacc
     3301 ccttagttag gagttatgat gagtcaaatg aactggagcg agcgcctggt tctggccttt
     3361 cacgccgtcc tgatccttgg tctactgcta gcgattggta gtctgaccgc cgatctagcc
     3421 gtgctgctgt cgatcagtct gatcatcgcc atcatcatct tccggccggt attgaaggtc
     3481 tctcgcctgt ttcagcgcac ctagaacaac cttcgataga aacgacggcg tcccacgcgg
     3541 gggttttact actcctccgc gtggggcaaa ccgccgagtt tagctaaaag caaactcggc
     3601 cttttttata accaactaac gcttgaccga cagataaaaa acagatacaa tggcgtttga
     3661 cttatgaaaa aagacttaca tccaaccgag taccgaccag tcgtcttcca ggacgattcg
     3721 gctggttttg cgtttttgac tcagtcaacc gcaaccacca cagacactat caaatgggaa
     3781 gacggcaacg agtatccact agttaagacc cacatctcca gtgcctctca cccttacttc
     3841 accggtgaag aaaagattat tgacaccgaa ggtcgggttg accgcttcaa ggctaagttc
     3901 gccgcagccg aagcccgcaa agatcagctg gctaacaagg ccaagaaaaa cgccaagcgc
     3961 gccgaagcca aagcccagag tgctaaaacc ggtcaaaacg aagccgccac tactaacaaa
     4021 gccggcgacg acactaaaaa atagctatct cgctcaaaaa ccggctgcct tatacggccg
     4081 gtttttaaat tggcaaacaa gataatgcta ggataaaggt aatatgccga agataaattt
     4141 agacctcaac gccttgaaag acgagaagca tcagctagaa ggattactgg ctcgtcctga
     4201 tgctttcgca cactctgatt tttcacaaaa atcaaagcgt ttgagcgagc tcagtgagct
     4261 gatcgatctg gctatcggtc gagacaaact aattgccgaa ttggatgaga ccaggcagct
     4321 ggctggcggt aacgatgagc tagccgatct ggcgaaagcc gaggtacctg aactggaaac
     4381 taagatcgaa gccgtcgatg agaaattatt tataatgttg acaccgaagg atcccaacga
     4441 cggcaaagat gtcatcgttg agatccgagc cggtgccggt ggtgatgaag ccagcctatt
     4501 tgccgccgaa ctgctgagaa tgtacctcag atactgcgaa acccacggtc tgaagaccga
     4561 actgatgagc gaaagttcgt ctgacgccgg tggctacaaa gaagtcgtgt tctcggtagt
     4621 tggcgatcag ccttacgctc agctgaagtt cgaatccggt gtccatcgtg tccagcgtgt
     4681 tccagctaca gagagtcaag ggcgtatcca cacctcaacc gtcacggtcg ccgtcatccc
     4741 ggaagccgaa gaggccgata tcgacatcaa cccgaatgat ttacgcatcg acatattccg
     4801 agcctccggc cacggtggcc aaagtgtcaa cactaccgat tcggccgttc gtatcactca
     4861 cttgccgtcg ggtctggttg ttagtaacca agacgaaaag tcacaaatta aaaacaagga
     4921 aaaagctatg aaggtactac ggtctcgcct gctccagcag aagatggacg aagaggccgc
     4981 caaagccgcc gaacaccgtc gttctcttat aggttccggc gaccgtagtg aaaagattcg
     5041 tacctacaac tttccccaag accggataac cgatcatcgc atcaagtact ctcgctccaa
     5101 catcccggcc gccctagacg gctctatcga cgatctaatc gaccacttag ccgcttatga
     5161 acgagaactc aaagcccaag gcgccaacgc ttagggactg gctggggtcg gcgcagagtc
     5221 ggctgaaggc cgtcggcatc agttcggctc ggctggacgc tgaattaatt ttgagttcaa
     5281 tcttaggcaa agacagagct tggctgttgg ctcacgatga tatgatgatg tctacgccga
     5341 acgccagcgc gggtggcgcg gcaggagggc gcccagtcgc gcgcagcacc tctggtgcga
     5401 gcacgatggg aggaagcctg gagcgacagg acccgcagac agcggagcta cgtggcgacg
     5461 gaacgtcgcc gacgacgcga a
//
LOCUS       DASSBW010000027         5940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_101287, whole genome shotgun sequence.
ACCESSION   DASSBW010000027 DASSBW010000000
VERSION     DASSBW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101287"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3177
                     /locus_tag="VFG56_00750"
     CDS             <1..3177
                     /locus_tag="VFG56_00750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET6622446.1"
                     /translation="GTGKTELLSLRVANILKRTDTAPGSILCLTFTDSGALAIRERLA
                     GLLGADAYKVAVHTFHSFGSEIINRHPEYFYNGARFQPADSLSTYKILKNIFDQLPHD
                     NILSSKMNDEYTYLKDVTSAISHIKKGGLFPDELAKIADNNLAFADWLKPELNRIFGA
                     TVSKKVIPAVVELIDMIGTYEEAELSVRTYHPLYRQIKTELELALSEVEASDKATPLS
                     RWKSKRLKKNGAGHYELKDAQNSRKLLALSDIYRQYLSQMEAHGLYDYDDMILRVINQ
                     LELAPDLKFSLQEQYQFIMIDEFQDTNDAQMNLVWQLIDNPVNEDRPDLMIVGDDDQA
                     IYRFQGASNSNIIDFNRRLPQRRLITLTDNYRSDEAILKLARQVIVQGEDRLERVIGG
                     LDKELAAHHHAAKPQLTYDMHPQSADEYSQLANLIAKSQQAKPTASRAIIARRHQTLK
                     DLVPYLQAKELPISYEMQDDALKSPPIEQLLLLCRIIHYLDEGRLSQADQLIPELLAH
                     PAWRIETKSLWQISLGARRLRTGDWLSLMLESDNSLLKDIATWFIELVKSASLDNMEL
                     MLDKLIGNQPVGDFTSPLKSYYFSRQRLDEQPAAYLSYLNNLRAIRQALRDYNSTRPL
                     KLTDFIEFVDLHLELKLPITAKYSVGDESSGINLLSAHQAKGLEFDEVYLVDFIDKRW
                     GETTRSPVDKLAFPANLPLKFQTDDTDEKLRLIYVALTRAKDKLCLSGPAFEMSGKTC
                     LPVGYLSGLLEAAQSEDRVGLEPAIGQARFDWQSSYLDLDRQSQASLLEPVLKRYRLN
                     ASHLNSFLNVTKGGPKQFLLDQLLHFPQAASSSAAFGLAIHEALRLAHDHFGATGEVQ
                     PQEDVTGNFLRELKSYQLADNEFDRLHGRGLKALSAYLDDKHTNFSGDQLAEVDFTSE
                     DIHLGDVRLTGKIDLMHVDRDAKTITVIDYKTGSACSNWQGKTAHDKIKMHHYRYQLL
                     FYKVLIESSSRFAGYTVDRGVIEFVEPTDQGTIERVELDYDQSEVELIKNLMTAVWQR
                     IQTLDFTLDKDYGEKLSDILDFEANLTK"
     gene            3231..3431
                     /locus_tag="VFG56_00755"
     CDS             3231..3431
                     /locus_tag="VFG56_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622447.1"
                     /translation="MSLVEQATRELREVEVEINTARYNLEQTEKSGDSRAVKQAHKQL
                     RKLEQQRMKLFKKVNRARSRGN"
     gene            3440..3649
                     /locus_tag="VFG56_00760"
     CDS             3440..3649
                     /locus_tag="VFG56_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622448.1"
                     /translation="MSLQDQLESAEGHFRAAHAACERAKKRVKRRHGKPADIASAKKE
                     LARAERELADSLKEVRRLRRRALAA"
     gene            3789..3971
                     /locus_tag="VFG56_00765"
     CDS             3789..3971
                     /locus_tag="VFG56_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622449.1"
                     /translation="MSQLGLFDPVGNDELNDAIANGKGGGHLTPRQISALEAAKKQAG
                     PQGRQAAEALRKAGKR"
     gene            4349..5296
                     /locus_tag="VFG56_00770"
     CDS             4349..5296
                     /locus_tag="VFG56_00770"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HET6622450.1"
                     /translation="MNSFWDELPKPFFVLAPMEAVTDVIFRHVIKRAATPDLFFTEFT
                     NATGWAHAGEPAIGGRLVKTEDEKPMIAHLWGADPEAMVKLAAHARDLGYDGLDLNTG
                     CPDKSAVRSGGGAGLIKTPELAAEMIKAMKTAGLPVSVKCRLGYSKLDEFEDWISFLL
                     SQDITALTVHLRTKKEMSKVDAHWEVMPRIVELRNEIAPRTLLIGNGDVRDRQHGLDL
                     IKQTGIDGVMIGRGVFHNPFAFGRQPEQHSKKELLDLLRLQLDLYDQYSPLTRRPFDT
                     LKRFFKIYVRDFDGAAELRDKLMHTKTTDEVRHILVSVN"
     gene            complement(5680..>5940)
                     /locus_tag="VFG56_00775"
     CDS             complement(5680..>5940)
                     /locus_tag="VFG56_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET6622451.1"
                     /translation="DSDREREYFAMTGGVIEVADNSLTVLVDEADHAADINRAEAEAA
                     HQRALDEKAKASTQVELDKAHSLIDRHAVRLRVADLHHRKRR"
BASE COUNT         1552 a         1569 c         1481 g         1338 t
ORIGIN      
        1 ggtaccggta agaccgagct gctcagtcta cgagtcgcca atatcttgaa aagaaccgac
       61 actgcaccag gcagtatact gtgtctgact tttaccgatt ccggcgccct ggctatccgc
      121 gaacgattag ccggcttgct cggtgctgac gcctacaagg tcgctgtaca cacctttcat
      181 agtttcggct ccgagataat caaccgccat cccgaatact tctacaatgg tgcacgtttc
      241 cagccggctg actctttgag tacctacaag attctcaaaa acatttttga tcagctaccg
      301 cacgacaaca ttttgtccag caagatgaac gacgaataca cttacttaaa agatgtcaca
      361 tcggccatct ctcacatcaa gaaaggtggt ttattcccag acgagttggc taaaatcgcc
      421 gacaacaatc tggcttttgc agattggctg aagccagagc taaaccgtat ctttggtgcc
      481 acggtatcaa aaaaggttat tcccgccgta gtcgaactaa tcgacatgat aggcacctac
      541 gaagaagccg agctgagtgt tagaacttat catccacttt accggcaaat caaaactgaa
      601 ctagaactag ccctaagtga agttgaggcc agcgataaag ctacgccgct tagccgttgg
      661 aaatctaagc gactaaagaa gaatggcgct ggtcattacg aattaaaaga tgctcaaaac
      721 agccggaagc tattggcgct gagcgatatc taccgacagt acttaagcca gatggaagct
      781 catggtttat acgactacga cgacatgatc ctgcgtgtca tcaaccaact ggaactggcg
      841 ccagatctca aatttagcct acaagagcag taccaattca tcatgattga cgagttccaa
      901 gataccaatg atgcgcagat gaatctggtc tggcagttga tcgacaaccc ggtcaacgaa
      961 gatcgacccg atctgatgat cgtcggagac gatgatcagg caatctatcg cttccaaggt
     1021 gccagcaact ccaacatcat cgacttcaac cgacgtttgc cacagcgcag attgatcaca
     1081 ctgaccgata actaccgctc agatgaagcc atcttgaaac tggctcgtca ggttatcgtc
     1141 caaggtgaag ataggctaga gagggtgatt ggagggctcg acaaagaact cgcggcccat
     1201 catcatgccg ccaaacctca actgacctat gatatgcatc ctcaatcggc cgacgagtac
     1261 agtcaactgg caaatttgat cgccaaaagt cagcaggcga agccgacagc tagccgggcg
     1321 ataattgccc ggcgccacca gactctaaaa gatctagtgc catacctaca agccaaagag
     1381 ctaccgatta gctacgagat gcaagacgat gccctaaaaa gcccgccgat cgaacaacta
     1441 ctactgcttt gtcggattat tcactatttg gacgaaggcc ggttgtctca agccgatcag
     1501 ctaattcctg agctgctagc tcacccggct tggcggatcg aaaccaagtc gctctggcaa
     1561 atatcgttag gcgcacggcg attacggact ggtgactggt taagcctgat gctggagtcg
     1621 gacaattcgc tactaaagga tatcgccact tggttcatcg aactggtcaa atcagccagc
     1681 ctcgataaca tggaattgat gctcgacaaa ctgatcggca accagccggt cggtgatttc
     1741 acctcgccgc ttaaaagcta ctacttctct agacagcgcc tggacgagca accagccgca
     1801 tatcttagtt atctgaacaa tctacgggct atccgccagg ccttgcgtga ctataactct
     1861 acccggccac tcaaactaac tgacttcatc gaattcgtcg atttacactt ggagcttaaa
     1921 cttccaataa ccgccaagta cagtgtcggt gatgaatcgt cgggcatcaa tctgctgagc
     1981 gcccaccagg ccaaaggctt ggagtttgat gaggtttatc tggtcgactt catcgacaaa
     2041 cgctggggcg aaactacgcg ctcaccggtt gataagctag cttttccggc caatttaccg
     2101 ctcaaattcc agactgatga caccgacgaa aaattgcgcc ttatctacgt agccctaact
     2161 cgcgccaagg ataaattatg cttaagcgga ccggcctttg aaatgtccgg taaaacctgt
     2221 ttgcccgtcg gttatctgtc cggcctgctc gaagctgcgc agtcagaaga cagggtaggg
     2281 cttgagccgg ccatcgggca ggctcggttt gactggcagt caagttatct ggatctcgac
     2341 cgccagagtc aggccagtct actggagccg gtgctcaagc gctaccgttt aaatgcctct
     2401 cacctcaata gctttctcaa cgttaccaaa ggtggaccta aacaattttt gcttgaccaa
     2461 ttgctgcatt ttcctcaagc agcaagctcg tcggctgcgt tcggcctagc tatacacgaa
     2521 gccctacgtt tagcccacga tcacttcggc gccaccggtg aagttcaacc ccaggaagac
     2581 gtcactggca atttcttaag agagcttaag agctaccagc tggccgacaa tgagtttgac
     2641 cgactacacg gccgaggcct caaagccctg tcggcatatc tggatgataa gcatactaac
     2701 tttagtggag atcaactggc tgaagttgat ttcaccagcg aagacatcca cctcggtgat
     2761 gtccgactga ccggcaaaat cgatttgatg catgtcgaca gagacgccaa gactataacc
     2821 gtaatcgatt acaagaccgg ctcggcttgc tcaaactggc aaggtaaaac agcccatgac
     2881 aagattaaga tgcaccatta tcgctatcaa ctgttgttct acaaggtatt gatagaaagc
     2941 tcgagccgtt tcgccggcta taccgtagac aggggcgtaa tcgagtttgt cgagcctacc
     3001 gaccaaggca cgatcgaacg agtggagctg gactacgacc aatcggaagt tgagttaatc
     3061 aagaacctga tgactgcagt ctggcaacgg attcagacgc tcgatttcac attggataag
     3121 gactacggcg aaaaactaag tgatattctc gatttcgaag caaacttgac taagtaggtc
     3181 tttcacttaa taatatccat attgtacctt gacaagaggg agtgatactc atgagtctag
     3241 tcgagcaagc cacccgagaa cttcgagagg ttgaagtcga gatcaacacc gctcgataca
     3301 atcttgaaca gaccgagaaa tccggcgaca gtcgggcggt caagcaagcc cacaagcagc
     3361 tacgcaagct ggagcagcaa cgcatgaagc tgtttaagaa ggtcaatcga gcccgtagtc
     3421 gaggaaactg aggtcagcta tgtctctcca agatcaactg gaatcggctg aaggccactt
     3481 cagggcggct catgcagctt gtgaacgtgc caaaaaacga gtcaagcgac gccatggcaa
     3541 accggccgat atcgcaagcg ccaaaaagga gctggcaaga gccgagcgtg agctggctga
     3601 ctccctcaag gaagttcgtc gactgcgtcg tcgtgctctt gccgcttgac tagcaacgag
     3661 cggtccaagc ggtcgcactt tgacttaaag tgctgccgct tggactatgc ttgattattt
     3721 ctcgcctaac gggtataatt tagaccactt gtgaggtgga tggtccattt cgcttgtacc
     3781 tacaggaggt gagtcagttg ggtcttttcg acccggtcgg aaacgacgaa ctcaacgacg
     3841 ccattgccaa cggcaagggt gggggccacc tcactccgcg ccaaatcagc gccctcgagg
     3901 cagctaagaa gcaggccggt ccacaaggcc gccaagctgc cgaagccttg cgcaaggctg
     3961 gtaaacgctg atgtaaagca gccgccggcc ggttggcctg gctagtcacg tacccgagat
     4021 acgtgatgtt tgatgccagg tcagccgcgc cgcgcggcgt ccacgccggg gcgggttgcc
     4081 aattcccccg tatggcgacc cgctccggtc ggagcaaagt cgggcgtagt ctttgatcca
     4141 aaaaagactg cggctagtag cgcccagcga tgaggtgctc gccgcgatcc ggattcatca
     4201 gggaggggaa gcccaatccg tgatccgccc gacgaacgtc taaccgacgc ttcactctgc
     4261 atttcagagc agaaccggtg acgtaaaccc gctttttaag taaagcgggt tttttaatgc
     4321 ctgaaacttt atgagataat aagtaataat gaattctttc tgggacgagc taccaaagcc
     4381 cttttttgtg ttggcaccga tggaagcggt gactgacgtg atttttcgac atgtcatcaa
     4441 acgtgcggct acaccggatc tgtttttcac cgagtttact aacgccactg gctgggctca
     4501 tgccggtgag ccagctatcg gtggacggtt ggttaaaacc gaagacgaaa agccaatgat
     4561 agcccatcta tggggtgccg accccgaagc tatggtcaaa ctagccgccc atgcccgtga
     4621 tcttggttac gacggtttgg atcttaatac tggttgtcct gacaaatccg ccgttcgctc
     4681 tggtggtggg gcaggactga tcaaaacgcc ggaacttgcc gctgagatga taaaagctat
     4741 gaagactgct ggcctgccag tcagcgtcaa gtgtcgcctc ggttactcca aactagacga
     4801 gtttgaagac tggatcagtt ttttactaag ccaggacatt accgcactga ccgtgcacct
     4861 gcgaaccaag aaagaaatga gcaaagtcga tgcccactgg gaagttatgc cgcgtatcgt
     4921 ggaattacga aacgaaatag cccctcggac actactgatc ggaaatggtg atgtccgcga
     4981 tcgtcagcac ggtctggatc taatcaagca aaccggcatt gatggcgtca tgatcggccg
     5041 cggcgttttt cacaatccgt ttgcttttgg gcgccagcct gaacaacaca gcaagaaaga
     5101 gttgctcgac ctactcaggt tacagcttga tctatacgac cagtattctc cgctgacacg
     5161 tcggccattt gacacgctga agcgcttctt caagatctat gttcgcgact ttgacggtgc
     5221 cgccgaacta cgcgataaac tgatgcatac aaaaacaacc gacgaagttc ggcatatttt
     5281 agtgtcagta aattagcttt tttgctccgc gtagtcaccg atgacataag tcatagacac
     5341 gtagctgcgc tgtctcacac cgaccgtctc tctaacccta tcaagccggc gcttgcccgc
     5401 cattcctcct cagggacacg gaaatttaaa gattctaaat ttcctatgcc gggaaggcaa
     5461 gtccccttgg agaaaagccg gacatcgccg ttagcgcttg aggccacccg tcatctcttc
     5521 ctcatatcct ttgacgaacc caggagtact agtgatctac acccgaatta gcgagtacga
     5581 gcttgttctc cgcacatagg taaatctagt gtagttatca ctagtgcgac atggcgtgaa
     5641 gacaactgga tgtgagaaag atggtggtgg gtgataccgc tagcggcgct tgcggtgatg
     5701 taaatcggct acgcgtaagc gaacggcatg ccgatcgatt aggctgtggg ccttgtctaa
     5761 ttctacctgg gtggaggctt tggctttttc atcgagtgcc cgctggtgag cggcttcagc
     5821 ctcagcccgg ttgatgtcgg ccgcatgatc ggcctcgtcg actagaacag tcaggctgtt
     5881 gtcagctact tcaatcacac caccggtcat ggcaaaatac tcacgctcac gatcagagtc
//
LOCUS       DASSBW010000028         5039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_514980, whole genome shotgun sequence.
ACCESSION   DASSBW010000028 DASSBW010000000
VERSION     DASSBW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514980"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFG56_00780"
     CDS             <1..243
                     /locus_tag="VFG56_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET6622452.1"
                     /translation="RRVGGANYQIPFPIQGHRQEHFAMMWLVNSARERKGMPYNERLS
                     AEIVDAYNETGAAFKKKEDTHRMAEANRAFAHFARS"
     gene            251..2356
                     /gene="fusA"
                     /locus_tag="VFG56_00785"
     CDS             251..2356
                     /gene="fusA"
                     /locus_tag="VFG56_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6622453.1"
                     /translation="MADKIKVTDLKQYRNIGIIAHIDAGKTTTTEGILYRTGLSHKIG
                     AVHEGETTTDWMEQEKERGITITSAAVTCFWHDDKGKDIKINLIDTPGHIDFTVEVER
                     SLRVLDGAVVVFDGKMGVEAQTETVWRQANKYGVPRICFINKINQTGGDFYMSLKTIH
                     ERLSKRALPIHLPIGFEKDINGVVDLIKMKAYTYTDFTDHELVEGDIPADMVEKAKNA
                     RTLLVEAAVEADDALFEKYLESGEDAISEQELKDAIRKSILTGEFYVVTGGDGRGVIV
                     EKVLDLVADYLPTPIDVGETWGTHPKSGDEISRKPDSSEPFAGLAFKIATDPFIGKLV
                     FVRAYSGKLTSGSYVLNATTGEKERVGRIVRMHADKREEVDEVAAGDIAAVVGLKNTT
                     TGTTLCDLSNPILLESIDFPEPPVAIAIEPKTKADQEKMGIALQRLAEEDPTFRVHTD
                     EETGQTIISGMGELHLDIIVDRMKREFKVEANIGQPQVAFRESIKGTAQVQGKHAKQS
                     GGRGQYGDVWVKFEPNESGKGFEFIDQIKGGVVPQEYRPAVQAGIKETLEGGVIAGYP
                     VVDVKATLYDGSYHDVDSSELAFKLAGTLATKEGVKQANPILLEPVMKVEVTTPEEFM
                     GDVIGDLNSRRGRIESMEDRLGAKLVTGFVPLAAMFGYTTDLRSMSQGRANSTMELHA
                     YEEVPPNVAQEIIEKRTAK"
     gene            2451..3278
                     /locus_tag="VFG56_00790"
     CDS             2451..3278
                     /locus_tag="VFG56_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622454.1"
                     /translation="MSVYRNGSYVGVSGLVNREQQRELTDYAGNTLGNRTDTMLFLGV
                     KAVHKTQWLDIENKYGPSWYPVGEEAFADTLDVGSDAVAQMYLDPEILAANPQYGTEF
                     IRRVKRRGHHWLNIIQFDLMPYRQNPEAFGYLIHEARREAGLREYKVIVQCHGEAMAE
                     GPKPAIEKLKRLSPDEIDWVLFDASHGKGLEMEPDALRPFLETGLEDPDLAHVGFGVA
                     GGLDAAAVERYIPELLRDFPEISWDAEGRLHRTDDGVLDMQAAKDYLRASGDVIEAA"
     gene            3429..4613
                     /gene="tuf"
                     /locus_tag="VFG56_00795"
     CDS             3429..4613
                     /gene="tuf"
                     /locus_tag="VFG56_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET6622455.1"
                     /translation="MADFDRSKPQVNVGTMGHVDHGKTTLTAAITTVLAKKLPSDVNK
                     AVDYDKIDNAPEEKQRGITIATSHQEYESEKRHYAHVDMPGHADYVKNMITGAAQVDG
                     AILVVSAADGPMPQTREHVLLAHQVGVPKIVVFLNKMDMADEELVELVEMDVRELLTK
                     NGYDGDNAPIIKGSALKALEGDAKYEDAIMELVAAMDDYIEEPKRDLDKDFLMPIEDV
                     FSIKGRGTVATGRIEQGIVKLNDEVEIVGIRDTKKTTVTGIEAFKKNLDQGQAGDNAG
                     LLLRGIERNDIERGQVLAKPGTITPHTEFDAEVYVLTKEEGGRHTPFFKGYKPQFYFR
                     TTDVTGEVELPADKEMVMPGDTVTFKVKLLAAIAMDEGLTFAIREGGRTVGAGVVTKI
                     NK"
BASE COUNT         1290 a         1252 c         1353 g         1144 t
ORIGIN      
        1 cggcgagtcg gtggtgctaa ctaccagatt ccattcccga ttcagggcca ccgtcaagaa
       61 cactttgcca tgatgtggct ggttaactcg gcgcgagaac gcaaaggtat gccgtataac
      121 gagcgcctaa gtgcagaaat cgtcgatgct tacaacgaga ccggtgccgc cttcaagaag
      181 aaggaagaca cccaccgtat ggctgaagct aaccgcgcct tcgcgcactt tgcaaggagt
      241 taatttaact atggcagata agattaaagt tactgacctg aaacaatacc gaaacatcgg
      301 tattatcgcc cacatcgacg ccggtaaaac gacgacgacc gagggtatct tgtaccgtac
      361 cggcttgagt cacaagatcg gtgcagtcca tgaaggtgaa actaccaccg actggatgga
      421 gcaggaaaaa gaacggggaa tcactatcac ctcggctgct gtcacctgct tctggcacga
      481 cgacaagggc aaagacatca agattaacct tatcgacacc ccgggccaca tcgactttac
      541 agtcgaggtg gaacgttcgc ttcgcgtact cgacggtgct gtcgtcgtat ttgacggcaa
      601 gatgggtgtt gaagctcaga ctgagactgt atggcgtcaa gccaacaagt atggtgtgcc
      661 acgaatctgt ttcatcaaca agattaacca gaccggtggt gacttctaca tgagcctgaa
      721 gaccatccac gagcgtttgt ctaagcgagc tttgccgatc cacttgccga ttggtttcga
      781 gaaagatatc aatggtgtcg ttgacttgat caagatgaag gcctacacct acaccgactt
      841 taccgatcac gaattggttg aaggcgacat cccggccgac atggttgaaa aagctaagaa
      901 tgcccgcacc ttgctggttg aagccgccgt tgaagccgac gacgctttgt tcgagaagta
      961 cctcgagtcc ggcgaagacg ccatctccga acaagagcta aaagacgcta ttcgcaaaag
     1021 catcttgacc ggtgagttct acgtcgttac cggtggtgac ggtcgtggtg tcatcgttga
     1081 gaaggtactc gatctagtag ccgactattt gccgacacct atcgatgtag gtgagacctg
     1141 gggtacccat ccgaagtctg gcgatgagat tagccgtaaa cccgacagtt cagaaccgtt
     1201 tgccggtctg gcctttaaga ttgctaccga tccattcatc ggtaagctgg tctttgtccg
     1261 ggcttactcg ggtaagctga cttccggtag ctacgtctta aatgctacta ctggtgagaa
     1321 agaacgggtt ggtcgaatcg tgcgtatgca cgccgacaag cgtgaagaag tcgacgaagt
     1381 cgctgccggt gacatcgctg ccgtggtcgg tcttaaaaat accactaccg gtacgactct
     1441 gtgtgacctg tcgaatccta tcttgctaga gtcgattgac ttcccagaac cgccggtggc
     1501 tatcgccatc gagccgaaga ccaaagccga ccaggaaaag atgggtatcg ctctgcagcg
     1561 tctagctgaa gaagacccga ctttccgggt tcacaccgac gaggaaaccg gccagaccat
     1621 catttccggt atgggtgagt tgcacctcga catcatcgtc gaccggatga agcgcgagtt
     1681 taaagtcgag gctaacatcg gtcagccaca agtggctttc cgcgagagca tcaaaggtac
     1741 cgctcaggtc caaggtaagc acgccaagca atctggtggt cgcggtcaat acggtgacgt
     1801 ttgggtcaag tttgaaccga acgagtccgg taagggcttc gagttcatcg atcagatcaa
     1861 gggtggtgtc gtgccacagg aataccgacc agccgttcaa gctggtatca aggaaaccct
     1921 agagggtggt gtcatcgctg gttatccggt tgtagacgtc aaagcaactt tgtatgacgg
     1981 tagctaccac gacgttgact ccagtgagct ggcctttaag ctagccggta ctcttgccac
     2041 caaggaaggt gtcaagcagg ctaacccaat cctgttggag ccagtcatga aagtcgaagt
     2101 taccactccg gaagagttca tgggcgacgt catcggtgac cttaactctc gtcgcggtcg
     2161 gatcgaatcg atggaagacc gcttgggtgc caagctggtt accggctttg tgccactggc
     2221 tgcaatgttt ggttacacta ctgacctgcg tagtatgagc cagggccgag ccaactcgac
     2281 catggagcta catgcatacg aagaagttcc accaaatgta gctcaggaaa ttatcgaaaa
     2341 gcgtaccgcc aagtaaatag ttttcagctg acaatacgaa taccctcggt ttgctgaggg
     2401 tattcgttta tgcttgattg gccaccgccc ttaagcgata ctgggggtta atgagtgtct
     2461 atagaaacgg atcttacgta ggcgtaagtg gtctggtcaa tcgtgaacag cagcgtgaat
     2521 tgacggatta cgccggcaat actctcggca accgtaccga tactatgctg tttcttggcg
     2581 tcaaggcagt acataaaact caatggctcg atattgaaaa taagtatggc cctagctggt
     2641 atccggtcgg tgaagaagct tttgccgata cgttggatgt cggcagcgat gcggtggcgc
     2701 agatgtacct cgatccggag atcttggctg ccaacccgca gtatgggact gagtttattc
     2761 gccgcgtcaa gcgacgtggc catcactggc tcaacatcat ccagttcgat ctgatgccgt
     2821 accggcagaa tccagaagct tttggctacc tgatacatga agctagacgc gaggcaggcc
     2881 ttagggagta caaggtcatc gtacagtgtc acggcgaagc catggctgaa ggtccgaaac
     2941 cggctattga gaagctaaaa cgattatcac cagatgagat agactgggta ctgtttgacg
     3001 cctcgcacgg taaaggcctg gagatggagc ccgacgctct caggcctttc ttggagacgg
     3061 gattagaaga cccggatctg gcccatgtcg gctttggcgt agccggtggg ctcgatgccg
     3121 cagccgttga gcggtacata ccggagttac taagagactt tccggaaatc agctgggacg
     3181 ctgaaggccg tctgcatcgt acagacgacg gcgtcttgga tatgcaggca gctaaagatt
     3241 atttacgtgc ttcgggcgat gttatagaag ccgcctaagc gggcagggtg ggccgcgata
     3301 cgccagagta actaatcaga tgcaaactgt ttacttttag gagtggattc actataatct
     3361 gacgcatacg tgtctagtga gtcctgctcc tatgcaccaa attaattaac taaggagaaa
     3421 taacactaat ggcagacttt gatcgaagca agcctcaagt caacgtcggt accatgggtc
     3481 acgtcgacca cggtaaaacg actttgactg cagctattac cacggttcta gctaaaaaat
     3541 taccaagtga tgtcaataaa gctgttgact acgacaagat cgacaacgct ccagaagaga
     3601 agcagcgtgg tatcaccatc gctacctctc accaagaata cgagagtgag aagcgtcact
     3661 acgctcacgt cgacatgcct ggtcacgccg actacgtcaa aaacatgatt accggtgccg
     3721 cacaggttga cggtgctatc ctggttgttt cagccgctga cggccctatg cctcagactc
     3781 gtgagcacgt acttctagct caccaggttg gtgtgccgaa gattgtcgtg ttcctaaaca
     3841 agatggacat ggccgacgaa gagctagtcg agctagtcga gatggacgtt cgtgagttgc
     3901 tcaccaagaa cggttacgac ggcgacaacg ctccgatcat caagggttct gccttaaagg
     3961 ccctagaagg tgacgctaag tacgaagacg ccatcatgga attggtcgcc gctatggacg
     4021 actacatcga agagccaaag cgcgacctcg acaaagactt cctgatgccg atcgaagatg
     4081 ttttctccat caaaggccgt ggtactgttg ctaccggtcg tatcgagcag ggtattgtta
     4141 agctgaacga cgaagttgaa atcgttggta ttcgcgacac taagaagact accgttaccg
     4201 gtatcgaagc cttcaagaag aacttagacc aaggtcaagc tggtgacaac gccggcctac
     4261 tccttcgcgg tatcgaacgt aacgacatcg aacgtggtca ggtcttggct aagccgggta
     4321 ctatcacccc gcacaccgaa tttgacgccg aagtctatgt tctgaccaag gaagaaggtg
     4381 gtcgtcacac tccattcttc aagggttaca agcctcagtt ctacttccgt actactgacg
     4441 taaccggtga agttgagctg ccagctgaca aagaaatggt catgcctggt gacaccgtaa
     4501 ccttcaaggt taagttgctt gccgctatcg ccatggatga aggtttgacc ttcgctatcc
     4561 gtgagggtgg tcgcaccgtt ggtgctggtg tcgtcacaaa gattaacaaa taatctcaga
     4621 cgaacagaac accaactaag aagtcccggc aaagccgggc tttttagttg gcttgcttgc
     4681 tacttccgcg ttttttctac ggcttcagct agggcagtag ttactacagc cctagccgtc
     4741 gtcctcgaaa aaaacacaaa attagctaag cctctgtccg cctgatagtc ggtgcattgt
     4801 ccaatgcgtt actcgctggc gagtctcgat atacccttat tcaagaaaca caagtgcctc
     4861 cttacttggt tgttctcact acaggcattc ctgaaaatga ccgagggcac tctacggtta
     4921 cgtgataata gttcagtctt caggtagaat gagtgcacta tggatgatgg agtggagcaa
     4981 ggcgggaaac caacgtgccg gttggaagag ctaggccggc tgagtcgtgc tggcgagag
//
LOCUS       DASSBW010000029         4383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_94756, whole genome shotgun sequence.
ACCESSION   DASSBW010000029 DASSBW010000000
VERSION     DASSBW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94756"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFG56_00800"
     CDS             complement(<1..629)
                     /locus_tag="VFG56_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET6622456.1"
                     /translation="MSKLSKRFWVSLVSLALVVLVLFFGRHQLEQAWELLASVNIWIL
                     LLLIPAQLISYHASGEIIFSYLNNKHNLGRLSRIDMTKLALEFNFVNHILPTGGVSGA
                     SYMTWRLKKLGVSSGRATMALFVRFAMGFAGFLTLLIFALFFMAIDDSLNRLILLASS
                     FLGSAIILVSLFVIFVIGSRARLEAVSHSIAKFVNQTMRKLSISWQIKEA"
     gene            673..1944
                     /locus_tag="VFG56_00805"
     CDS             673..1944
                     /locus_tag="VFG56_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6622457.1"
                     /translation="MQIAILGRQAGLSLAELEAIYGDGSIRPVGDYACLVDIDTNLPQ
                     DRLGGTVKSAEVLTTLKRADLGKAFAYLKSKAKSLHAELPDGKLKIGFSIYGLRAKPT
                     DLSKKALEFKKVLKASGRSVRIVEAKGLGLSAAQVLYNKLTAELGREVLILLDGSDVI
                     IARTTGVQDIDDYSARDFARPKRDARVGMLPPKLAQIMINMATGRWALDVGRWKTPDA
                     SVRSTERDSDLRGNSEGISDDGNGHTPRLLDPFCGTGVVLQEAALMGLSVYGSDIEKR
                     MIDYTRDNLVWLEDKYGLKADKFFEVADATDHIWRQPIDLIVCEGYLGQPFSTEPSRE
                     KLEDVRHTTGVIMRKFLSNIAGQLNPGTRLVIGAPVWFMGGRPHHLKMLDDLEDLGYN
                     RLSFVRASQKDLIYHREDQVVGRELLILTRK"
     gene            1946..2218
                     /locus_tag="VFG56_00810"
     CDS             1946..2218
                     /locus_tag="VFG56_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET6622458.1"
                     /translation="MSHVKAGGSSKNVHDSPGQRLGVKRFGGQKVSAGEVLVRQVGET
                     KQAGPGTYMSRNFTIHAGKNGVVGFKQVKTRKFTGKTLRRTQVVVE"
     gene            complement(2269..3297)
                     /locus_tag="VFG56_00815"
     CDS             complement(2269..3297)
                     /locus_tag="VFG56_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622459.1"
                     /translation="MENQNPQNPEQAEAGQDQLERLNRLSDGQFTVKRSNGNLETGWN
                     VVNMAADKDNQLIVELDQPEQRMTKWVRASDLLSWQPQMTDEATIETVPRSQLQARIA
                     GEAVVSPEGQEADQADPVTQEMADEAIEASGVNRPQSRLQERLGLVAKASEQPGPVEA
                     QTKPPEKLGLNNFELDLIGRLVHEINNQAYREVDSLDMNQTAMATYQGVMDGIHRGEG
                     EKQTAATLATLVAAGAVEDLPMSSHLLDTACQEVAKLVNPEEAGNLAYQLRQAIAYKD
                     RDDISRQDLAQEMYHRFSTMAGDSYLNDKPKVRAAVLAFAAGMTARDPQLSHAMMTNL
                     ASLHQAES"
     gene            complement(3386..3763)
                     /locus_tag="VFG56_00820"
     CDS             complement(3386..3763)
                     /locus_tag="VFG56_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6622460.1"
                     /translation="MDKKKILLVEDDRALAEVYQTRLELEGFDVQHVPNGEDALSSAT
                     KYKPDLIVLDAMMPKINGFDVLDILKQTPATANIRVVMLTALSQAKDKERAEQLGADD
                     YLVKSQVVISDVVARIKHHLGLQ"
     gene            complement(3803..>4383)
                     /locus_tag="VFG56_00825"
     CDS             complement(3803..>4383)
                     /locus_tag="VFG56_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6622461.1"
                     /translation="KVLDGNVGYLRISTFAKNTTDDARAAVEELKSKHVKGIILDLRS
                     NGGGYLEAAQGVASIWLGDNQTIVTERTGGEVTKKLTGYGNQLVGNMPTVVLVDGGTA
                     SASEIVAAALRDNGKAQLVGTKTYGKGTVQEPISLDDGGVLKVTVAHWYTPDGQSIDG
                     NGLKPDKKVEPTIKQIEQNNDVQLRAATELLK"
BASE COUNT         1058 a         1141 c         1089 g         1095 t
ORIGIN      
        1 gcctccttga tttgccaact gatgctcagc ttccgcatgg tctgattgac gaacttggct
       61 atagaatgcg aaacggcttc cagacgggca cgcgagccga taacaaagat gacgaacaag
      121 ctgacgagta tgatggccga acccaagaaa ctactggcta gtaagatcaa ccggttcagc
      181 gagtcgtcga tagccatgaa gaacagcgca aagataagta acgttaagaa gccggcaaag
      241 cccatagcaa accgaacaaa caacgccatg gtcgcccgtc cagagctgac gccgagcttc
      301 tttaggcgcc aggtcatata agaggcgccg gatacgccac cggtcggcag gatatggttg
      361 acgaagttga attctagtgc cagcttggtc atatcgatac ggctcagtct acccaaattg
      421 tgcttgttgt tgagataaga aaagataatt tcgccggaag catgataact gatcagttgc
      481 gccgggatga gtagtagcaa gatccaaata ttgaccgagg ccagtagctc ccaggcttgc
      541 tccagttggt gacggccaaa gaacagcact aatacaacca gggctagact gaccagactg
      601 acccagaacc gtttcgacag tttgctcata gcgactatta taaccgagac tttttaacgt
      661 tataattaat ccatgcagat cgccatactg ggccgccaag ccggtctatc actcgccgaa
      721 ctcgaagcca tttatggcga cgggtccatc cgccccgtcg gtgattacgc ttgtctcgtc
      781 gatatcgata ctaatttgcc gcaagatcga ctgggtggca ccgttaaaag tgccgaggtt
      841 ttaaccacgc tgaaacgagc cgacttaggt aaggctttcg cctatctcaa gtcaaaagcc
      901 aaatctctac acgccgaatt acccgacgga aagctaaaga tcggcttcag tatctatggc
      961 ttacgagcca aaccgaccga tctatccaaa aaggccctag agttcaaaaa agtcctcaaa
     1021 gccagcggcc gtagtgttcg catcgttgaa gccaaaggct taggactgtc cgccgctcag
     1081 gtgctctaca acaaattaac cgccgaactc ggtcgtgagg tattgatcct acttgatggc
     1141 agtgatgtca tcatcgcccg tactaccggc gttcaagaca tcgacgacta ctcggcccgc
     1201 gactttgccc gacccaagcg cgacgcccgc gtcggtatgc tgccgcccaa actggcgcag
     1261 atcatgataa atatggcgac tggacgttgg gcgttggatg tggggcgttg gaaaacaccg
     1321 gatgcgtcag tccggtcgac ggagcgggat agcgacctac gtggaaattc tgaaggaatt
     1381 tctgacgacg ggaacggtca tactcctaga ctgctggatc cattctgcgg cacaggcgta
     1441 gtactgcagg aagccgcgct gatgggcctg tctgtctacg gcagtgatat cgaaaagcgg
     1501 atgatcgact atacgcgtga caacctggta tggctggagg ataagtatgg cctcaaggcc
     1561 gacaaattct ttgaggttgc cgacgctacc gaccacatct ggcggcagcc gatcgacctg
     1621 atagtctgcg agggttatct tggacaaccc ttctcaaccg agccatcgag agaaaaactg
     1681 gaagatgtcc gtcatactac cggtgtaatc atgcgaaagt ttctgagtaa catcgccggt
     1741 cagctaaatc caggcactag actggtcatc ggcgcaccgg tttggttcat ggggggacga
     1801 cctcatcatc tgaaaatgct tgacgacctc gaggatttgg gttacaatcg attaagtttt
     1861 gtgcgcgcta gccaaaaaga tttgatctat catcgcgaag atcaagtcgt tggccgcgaa
     1921 ctgttaatac taacgaggaa ataatatgtc acacgtaaaa gctggtggtt catccaaaaa
     1981 cgtccacgac tccccgggtc aacgccttgg tgttaagcgc tttggtggcc aaaaagtctc
     2041 agccggcgaa gtcctagttc gccaagttgg cgagacaaaa caggctggtc ccggcactta
     2101 catgagccgt aatttcacta tccacgccgg aaaaaacggc gtcgtcggtt tcaagcaagt
     2161 caagactcgc aagtttaccg gcaaaacatt acgacgaacc caagtagtcg tcgagtaaac
     2221 gctacagcca taaataaaaa tgccccgtct ggggcatttt tatttagatt acgactccgc
     2281 ctgatgcaag gaggctaagt tggtcatcat ggcgtgtgat agctgcgggt cacgcgccgt
     2341 catacctgcg gcgaatgcca aaacagcagc ccggacttta ggcttatcgt ttaagtagct
     2401 gtcgcccgcc atagtcgaaa aacgatggta catttcttga gccaggtctt gtctcgatat
     2461 atcgtcacga tctttgtagg ctatggcctg tcgcagctgg taagctaagt taccggcttc
     2521 ctcaggatta accaacttgg ctacttcttg gcaggccgtg tcgagcaagt gactactcat
     2581 cggtaaatcc tcgacggcac cggcagccac tagtgtagct agggtcgcgg cagtttgctt
     2641 ttccccttcg ccgcggtgaa taccatccat aacaccctga taggtagcca tggcggtctg
     2701 gttcatatca agtgaatcaa cttctctata ggcttggttg tttatctcat gaaccaaacg
     2761 tccaatcaaa tccagctcga agttatttag gcctaacttc tcgggcggtt tggtttgagc
     2821 ctcaaccgga ccgggctgct ccgaggcctt agcaaccaag ccgagacgct cctgcaggcg
     2881 gctctgcggt cgattaactc cactggcttc aattgcttca tcggccatct cttgggtgac
     2941 cggatcggcc tgatcagctt cttgtccttc aggtgaaaca acggcttcac cagctatccg
     3001 ggcctgtagt tgtgatcgtg gtacggtctc tatagtagct tcgtctgtca tctggggctg
     3061 ccaagatagc agatcgctag ctctgaccca tttggtcatt ctttgctcag gctgatctaa
     3121 ttctacgatt agctgattgt ctttatcggc agccatattg accacattcc agcctgtctc
     3181 gaggttaccg ttactgcgtt tgacagtgaa ctgaccgtcg ctcaatcggt ttaatctctc
     3241 caactgatcc tggccggctt cagcttgttc tgggttttgt ggattttggt tttccatagt
     3301 ggtgatttat agacaatatt aacaagaaat aagcgttttg tcaatacgct cacgctcttg
     3361 ttaccgaaga cggcgggtta ttttactact gtaagccgag gtggtgcttg attcgcgcca
     3421 ctacatcgga gataaccact tgggatttga ctaaatagtc gtcggcaccg agctgctcgg
     3481 ctcgttcctt gtccttagcc tgcgacaacg ccgttagcat aactactcga atgttggcgg
     3541 tagctggagt ctgcttaagg atatcgagca cgtcaaagcc gttaatcttc ggcatcatag
     3601 cgtctaggac gataagatca ggtttgtatt tagtggcaga cgacaaggca tcttcaccgt
     3661 tagggacgtg ttggacatca aagccctcta gctccaaacg ggtttggtaa acttcggcca
     3721 gtgcccgatc gtcttcgact aatagaattt ttttcttatc catttgtgct tatactacct
     3781 catttaatca aatcatagca agttatttga gcaactccgt ggcggctctc agctgaacgt
     3841 cattgttttg ttcgatctgc ttaatcgttg gttcaacttt tttgtccggt tttaagccat
     3901 taccgtcaat cgattgacca tccggtgtgt accagtgcgc caccgtaact ttcagtacgc
     3961 cgccgtcatc aagactaatc ggttcttgaa ccgtgccctt tccgtaagtt ttggtgccga
     4021 ctaattgggc tttgccgtta tcccgaagag cggccgctac gatttcgctg gcgctggcgg
     4081 taccgccgtc gaccaaaaca accgttggca tgtttccaac tagttgatta ccgtagccgg
     4141 tcagtttctt agtcacctcc ccgccagtcc gctcggttac aatagtctgg ttgtcgccca
     4201 accaaatact agctactcct tgggccgcct ccaggtaacc gccgccattg cttctaaggt
     4261 ccagaataat acctttgacg tgcttggact tcagctcctc caccgccgcg cgcgcatcgt
     4321 ctgtagtgtt cttggcaaaa gtcgatatgc gtaggtagcc gacattgcca tccaaaacct
     4381 tgt
//
LOCUS       DASSBW010000030         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1756273, whole genome shotgun sequence.
ACCESSION   DASSBW010000030 DASSBW010000000
VERSION     DASSBW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756273"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFG56_00830"
     CDS             complement(<1..588)
                     /locus_tag="VFG56_00830"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6622462.1"
                     /translation="MKQPLAEQMRPQTLDEIIGQGHIVGDDGLLKKIVAKQQPTSLIL
                     WGPPGTGKTTLARILAHAWEADFVELSAVIAGKKDVLQVVERARQNWNLNQRTVLFVD
                     EIHRFNKAQQDAFLPHVESGLITLIGATTENPSFEIITPLLSRSRVLVLEPLSKDDML
                     AILKRALRLNKASKRVTPKALDYLAELSGGDARLAL"
     gene            642..1073
                     /locus_tag="VFG56_00835"
     CDS             642..1073
                     /locus_tag="VFG56_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HET6622463.1"
                     /translation="MKQQLLLADKVRVMLQAACATNRQLLEVALKDYRLSTAQAEVIE
                     ILSVEGQLNIKQIGHLITCEKSSPSRLLSRMKNQGLVYITKDPTDQRASLVQLTPRGE
                     QLAEVIDRVNFTIDINTSRRLSTNEQLQLEILLSKLSGIEP"
     gene            1111..2121
                     /locus_tag="VFG56_00840"
     CDS             1111..2121
                     /locus_tag="VFG56_00840"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET6622464.1"
                     /translation="MRTRVLLLFGGQSTEHEVSLSGAANVFAALDDQKYDISLGLIDK
                     SGKWYLVPEIDPDHTNCPQLVPVLGTSRFVTVPEGVILQPDVILPILHGQFGEDGTVQ
                     GLAKLLHIPIVGPSLLGAALTMDKQLTKTVLTAAGVPVVDGVLWRTVEEKPDYSKLAE
                     QLGEVMFVKPANAGSSVGVSKVRTAEEFVAALALAAEHDDKVLIERAIDGREVEVAVL
                     GNARPSTSQPGEIKANAEFYDYTAKYDPSSQAEVIVPADLDEATIKKIREFSSKAYQA
                     TAGHGMARVDFLIDEDGEAYVIEVNSIPGFTNISMYPKLWRERGLSYPALLDKLIELA
                     LE"
     gene            2125..2832
                     /locus_tag="VFG56_00845"
     CDS             2125..2832
                     /locus_tag="VFG56_00845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6622465.1"
                     /translation="MNQLYWLYALLTLLVLVIIYVLAGVYRVLVIDTRAYAKFWRELA
                     DQPSKKDELTVVALGDSTFQAIGATQPLLGTVGLSADYIEKQTGRPIHINNFSVTGAK
                     AQDVAESQLPQADLESADIVLVAVGANDALKESDLVVFESAIKQICEQLPANKSVMAD
                     VALVNHRRPYQKLLAKYRDQAGIYPADLSYSFGMVAQSWRLSARDFFHPSDYGYTFWF
                     KAFQPALDKLISRRLAK"
     gene            2974..3049
                     /locus_tag="VFG56_00850"
     tRNA            2974..3049
                     /locus_tag="VFG56_00850"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3007..3009,aa:Phe,seq:gaa)
     gene            complement(3119..>4033)
                     /locus_tag="VFG56_00855"
     CDS             complement(3119..>4033)
                     /locus_tag="VFG56_00855"
                     /inference="COORDINATES: protein motif:HMM:NF037498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide kinase domain-containing protein"
                     /protein_id="HET6622466.1"
                     /translation="YWQFAAKRQAIFEKRLIGAPRPWTDDPILQTYKFCNVYRATDRV
                     SQYMIREVCYGLQNDTPADRLFQIVAFRTFSRPETWEGIQKFLGHSPTLQDLADDSLT
                     KALEYVKQTDGKLYTHAFILCAADSYRQGAKYLNHVELFRHMFLSEDLTDKLLAAKSL
                     KEVYELLHTFPLMGDFMSYQTAIDLNYSDLINFSENDFTKAGPGALRGMKKVFSDFGG
                     LTPEQIIMWMVEHQDVEFKRLGLKFNGLFGRPLHAIDAQGLFCETDKYCREAIPDITS
                     ARSKIKSKFTATPEPIKLFFPPKWHIKN"
BASE COUNT          955 a          948 c         1090 g         1040 t
ORIGIN      
        1 gagtgctagt cgagcatcgc ccccagacaa ctcagctaga tagtcgagcg cttttggcgt
       61 cactcgtttg ctggctttgt ttagccttaa ggctcgtttc aatatagcaa gcatatcgtc
      121 tttgctgagc ggttcgagta ctaagacgcg agagcgagag agcaacgggg tgatgatttc
      181 aaaactgggg ttttcggtgg tggcgccgat tagtgtgatc aatcccgact ctacatgggg
      241 caggaaagcg tcctgctggg ctttgttgaa gcggtggatt tcgtcgacaa acagaacggt
      301 gcgttggttc aaattccagt tttgacgggc gcgttcgacg acttgaagca catctttttt
      361 accggcgatg acggccgata gctcgacaaa gtcagcttcc caggcgtggg ccaggattcg
      421 ggccaaggta gtcttgccgg taccaggagg tccccatagt atcaggctgg ttggctgctg
      481 cttggcgacg atttttttga gaaggccatc atcgccgacg atgtgcccct ggccgataat
      541 ctcatccaat gtctgcggcc gcatctgttc ggccagtggt tgtttcataa ttcaattata
      601 tcgagtttat aagtataagc atgatggttt ataatttaac tatgaagcag caactactct
      661 tagctgataa agtccgagtt atgctgcaag ccgcctgcgc taccaatcgt caactgcttg
      721 aggtggcact taaagattat cgactgagta ctgcccaggc ggaggtgatt gaaatcctga
      781 gcgtcgaagg acagctaaac atcaaacaga tcggtcatct gataacttgt gagaaatcca
      841 gtcctagtcg actgttgtct cggatgaaaa accaaggtct ggtctatatc accaaagacc
      901 caaccgatca gcgagccagc ttggtccagc taacacctcg aggcgaacaa ttggctgaag
      961 tgatcgaccg cgtcaacttc actatcgata tcaatacatc taggcgccta tcgaccaatg
     1021 aacaattgca gctagagatt ttactcagca aactgagcgg tatcgagccg taagagtcgg
     1081 caagttagtc cgtgttatga tgaaggtatt atgcggacac gcgtattact gctgtttggc
     1141 ggccaatcaa ccgaacatga agtctcgctc tccggggcgg ccaatgtctt tgcggcgctc
     1201 gatgatcaga aatacgacat ctcactcggg cttatcgaca aatccggcaa atggtatttg
     1261 gtgcctgaga ttgatccgga ccacaccaat tgccctcagt tagtaccggt gcttggcact
     1321 tcgcgttttg tcactgtgcc ggaaggtgtc atcttgcagc cggacgttat cctgccaatc
     1381 ttgcatggtc aattcggcga agacggcacc gtccagggtt tggccaaatt gctacacatc
     1441 ccgatagtcg gcccgagctt gcttggggcg gctttgacta tggataagca actaaccaaa
     1501 acagtgctga cagccgccgg agtaccagtg gtagacggtg tgctttggcg aacggtcgag
     1561 gaaaaaccgg actactctaa gctagcggaa cagttaggcg aagttatgtt tgtcaaacct
     1621 gctaacgccg gttcatcggt tggcgtcagc aaagttcgaa cggccgagga gtttgtcgcc
     1681 gcactggcct tagcagctga gcacgatgac aaagttctga ttgagcgggc tatcgatggc
     1741 cgagaggtag aggtcgcagt tctcggtaat gctaggccat caaccagtca accgggagag
     1801 atcaaagcca acgccgagtt ttatgactac accgccaaat atgacccaag ctcacaggcc
     1861 gaagtcatcg ttccggctga tctcgatgaa gcgacaatca aaaagatacg cgaatttagt
     1921 tccaaggctt accaggccac cgctggtcac ggtatggcaa gggttgattt cttgatcgac
     1981 gaggacggag aggcttacgt tatcgaggtt aattccattc ctggctttac taacatcagc
     2041 atgtatccaa aactatggcg tgaacgtggc ttgagctatc cggcgctttt ggacaaactg
     2101 atcgaattag ctcttgagta gccgatgaat caattgtatt ggctctacgc tttgctgacc
     2161 cttctggtgc tagtgattat ctatgtgctg gctggtgtct atagggtatt ggttattgac
     2221 accagggctt atgccaagtt ttggcgcgag ttagctgatc agcctagcaa gaaggatgaa
     2281 ttgaccgtgg ttgcactagg tgactcgacc tttcaagcta tcggcgccac acaaccgcta
     2341 cttggcacgg tgggtttaag tgcggactat atcgagaagc aaaccggtcg ccctatccat
     2401 atcaataatt tcagcgtaac cggcgccaaa gctcaggatg tagccgagtc gcagttgcct
     2461 caggcagatc tagagtcggc cgacatcgta ctggtggcag tgggcgccaa cgacgctctg
     2521 aaggagagtg atctagtggt ttttgaatcc gccataaaac agatttgcga acaattgcca
     2581 gcgaacaaga gcgtcatggc tgatgtggct ttggtcaatc atagacgacc ctatcagaag
     2641 ttgttggcca aatacagaga tcaggccggc atatatccag ctgatcttag ctatagcttt
     2701 ggcatggtcg cccaaagctg gcgtttaagt gcgcgtgatt tcttccaccc tagtgactac
     2761 gggtatactt tttggtttaa ggcctttcag ccggcgctcg ataagctgat cagccgtcgt
     2821 cttgccaaat aacctctgtt accgtacaat tatctaagtt cttacccttg ctagttgtag
     2881 ctaagggaaa agagaagcga acttggtttc gctttagcga aactcagctt tgctgatgag
     2941 cgttgagaaa aaccgtaggt ttgtctcaac taaggctctt tagctcagtt ggtagagcag
     3001 aggcctgaag agccttgtgt ccccagttca agtctgggag gagccaccaa agaagatgcc
     3061 cgattttatg tcgggcgttt tctttggacg gactttgtcc gtggcttgaa ctggatcttt
     3121 aattttttat atgccatttc ggcggaaaga atagtttgat cggctcgggc gtggcagtga
     3181 acttactttt gattttggag cgagcgctgg tgatgtcagg tatggcttcg cggcagtatt
     3241 tgtcggtctc acagaacagg ccttgggcgt cgatggcgtg tagtggtcgt ccgaagaggc
     3301 cgttgaactt aagccccaag cgcttgaatt ccacatcctg atgttcgacc atccacatga
     3361 tgatctgctc gggtgtcaga ccgccaaaat cactaaagac ctttttcata ccgcgcagcg
     3421 cccctggccc ggccttagtg aagtcatttt ccgagaagtt gatgaggtcg ctgtagttta
     3481 agtcgatcgc tgtctggtac gacatgaagt cgcccatcag cgggaaggta tgcaatagtt
     3541 cgtagacttc tttgagtgat ttggcggcca gtagtttgtc ggttagatct tcgctcaaaa
     3601 acatgtggcg gaacagctcg acgtgattga gatacttagc tccttggcga taactgtcgg
     3661 ccgcacatag tatgaaggcg tgagtgtaga gcttgccgtc ggtttgcttg acgtactcca
     3721 gcgccttagt cagactgtca tcggctaggt cctgaagagt gggggagtga ccgagaaact
     3781 tctgtatacc ttcccaagtc tccggccggc taaatgtccg aaaggctacg atctggaaca
     3841 atctatcggc cggcgtatca ttctgtaggc cgtagcatac ttcacggatc atatactgac
     3901 taactcggtc ggtcgctctg tagacattac agaacttata ggtttgcagt atagggtcgt
     3961 cggtccaggg tcgaggtgct cctatgaggc gtttctcgaa tatggcttgc cgtttggcgg
     4021 caaactgcca gta
//
LOCUS       DASSBW010000031         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_94611, whole genome shotgun sequence.
ACCESSION   DASSBW010000031 DASSBW010000000
VERSION     DASSBW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94611"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..416
                     /gene="rpsM"
                     /locus_tag="VFG56_00860"
     CDS             30..416
                     /gene="rpsM"
                     /locus_tag="VFG56_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET6622467.1"
                     /translation="MARIANVVIPDAKQVQIALTYIYGIGPKFAGDILSATKIEPTKR
                     VKDLTEAELKKLRDEIDSNYQVEGDLQRQVSGNLKRLKDIQSYRGLRHKNSLPSHGQR
                     TRTNARTRRGKKIAVGGTQKKAASKT"
     gene            427..825
                     /gene="rpsK"
                     /locus_tag="VFG56_00865"
     CDS             427..825
                     /gene="rpsK"
                     /locus_tag="VFG56_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET6622468.1"
                     /translation="MSEAKTTTKSKKKQRRSVPSGQLHIQATFNNTIITFSDPKGNVL
                     SASSAGACGFRGSKKGTAYAAQIAAEKATESAKSQYGLGKVDVFVKGVGLGRDAAIRA
                     LSNFEIQVESIKDVTGVPHGGTRPRKARRA"
     gene            831..1451
                     /gene="rpsD"
                     /locus_tag="VFG56_00870"
     CDS             831..1451
                     /gene="rpsD"
                     /locus_tag="VFG56_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET6622469.1"
                     /translation="MARDRSPIVKQSRREGYALHPKAHKIMAKKSGIPGEHAHGRQGK
                     QSLYAQQLREKQKVRRTYGLLEKQFTRLMKEADKSEGLSGEVLLELLERRLDNAVYRA
                     GFATSRRAARQLVSHGHFMLNGRRVDIPSIRLKAGDEITVRPKSTKSVYFTTGFEDMH
                     KNTNQPVLSWMKADAKKLVIKITGTPKREEAEPDINEQLIVEYYSR"
     gene            1477..2400
                     /locus_tag="VFG56_00875"
     CDS             1477..2400
                     /locus_tag="VFG56_00875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6622470.1"
                     /translation="MANSIHNPTLAAVTDNGSNSATFTVEPLHNGYGMTLGNSLRRVL
                     LSSIEGAAITAFSIDGVSHEFTTVKGVKEDVVDIMLNLKNVVLTCHADEPVELRLEKK
                     GAGVVTAGDIKATGDIEVINKDFVICTIDDPKTQVGFNLVVENGRGYRSIEESSVKRL
                     HGDMIALDATFSPVVRVRYKVENTRVGQITDFDKLLLTVDTDGSISPTDAFEEAAAIL
                     LNQYTALAGSTRVEAAPAANVAAQEDAGELMTPIEDLNLSARTTNALINNDIRTINDL
                     VTLSDTEMRELKGFGSKAMDEVKDKLAEMEL"
     gene            2404..2754
                     /gene="rplQ"
                     /locus_tag="VFG56_00880"
                     /pseudo
     CDS             2404..2754
                     /gene="rplQ"
                     /locus_tag="VFG56_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            2868..3305
                     /gene="rplM"
                     /locus_tag="VFG56_00885"
     CDS             2868..3305
                     /gene="rplM"
                     /locus_tag="VFG56_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET6622471.1"
                     /translation="MTKKTFSLKASEVNHVWTLVDATDAPLGRLSTVIAQRLTGKYKP
                     TFTPHMDDGDYVVVVNADKLIVTGDKEEAKTYYRHTGYPGGIKSRSLKEARAKDSTKL
                     IEAAVKGMLPRNKLLAERMKRLRVFTGEEHGHTAQKPVKLEIK"
     gene            3308..>3516
                     /gene="rpsI"
                     /locus_tag="VFG56_00890"
     CDS             3308..>3516
                     /gene="rpsI"
                     /locus_tag="VFG56_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET6622472.1"
                     /translation="MVKSNYFYGVGRRKSATARARLTGGKGVITINGQAAEAYLDGNQ
                     TLLAELTDPLALIGKQKELDVSLVIS"
BASE COUNT          992 a          902 c          878 g          744 t
ORIGIN      
        1 aaacctaaga ataagcagag gcaaggttaa tggctcgaat tgctaacgtt gttattccgg
       61 acgccaagca ggttcagatc gctttgacgt acatctacgg tatcggtccg aagtttgctg
      121 gtgatatttt gagcgctact aagatagagc caaccaaacg ggtcaaggac ttgaccgaag
      181 ccgaacttaa gaagctacgc gacgagatcg actccaatta ccaggtcgag ggtgatttac
      241 agcgccaggt ttccggtaac ttaaaacgtc tgaaagatat tcaaagttac cgtggactgc
      301 gacacaagaa cagtctgcca tctcacggcc agcgtacccg aaccaacgct cggacaagac
      361 gtggtaagaa aattgctgtc ggcggtactc aaaagaaagc agcaagtaag acttagaagg
      421 taagatatgt cagaagcaaa gactacgact aaatccaaga agaaacaacg ccgatctgtg
      481 ccgagcggtc agctgcacat tcaggcaact ttcaacaaca cgataatcac cttctccgat
      541 cctaaaggta atgttctcag cgctagctca gctggtgcct gtggtttccg cggatccaag
      601 aagggtacgg cctacgccgc tcagatcgca gccgaaaaag ctaccgaatc cgctaagagc
      661 caatacggtc tcggcaaagt tgacgtattc gtcaaaggtg tcggtctggg ccgtgatgcc
      721 gcaattcgcg cgttatccaa ttttgaaatc caagtagaat caatcaaaga cgtaactggc
      781 gtgccgcacg gcggcacaag gccaagaaag gcacggaggg cttaaacttt atggcacgag
      841 atcgttcccc aatagttaaa caatcgcgtc gtgaaggtta cgcccttcat cctaaagcgc
      901 acaagatcat ggcgaagaag tctggcatac cgggtgagca cgcccacggt cgccaaggca
      961 agcagagcct ctacgctcaa cagctgcgcg agaagcagaa agtccgccgg acatacggac
     1021 tactggaaaa gcagtttacc cgtttgatga aagaagctga caagtccgaa ggtctgtctg
     1081 gcgaagtcct actggagctc ctggagcgac gtttggacaa tgctgtatac cgagccggtt
     1141 ttgcgacttc tcgtcgggct gcccgccagt tggtcagcca cggtcacttt atgctaaatg
     1201 gccgtcgcgt cgacatccca tcgatccgcc tgaaagccgg cgacgagatc accgtccgac
     1261 ctaagagtac taagtcggtt tactttacta ccggatttga agacatgcac aagaacacta
     1321 atcaacccgt cttaagctgg atgaaagcag acgctaagaa actagtgatt aagattaccg
     1381 gcacgccgaa acgcgaagaa gccgagcctg acatcaatga acagctaatt gttgagtact
     1441 actcgcgcta aagattaaag gagataagcg aacatcatgg caaactcaat acacaaccca
     1501 actctcgccg ctgtaaccga caacggctct aacagcgcga catttactgt tgagccgttg
     1561 cacaacggct acggtatgac tctcggcaac agcttacgtc gtgttctact atcgagcatc
     1621 gaaggcgctg ctatcaccgc cttttctatc gacggtgtta gtcacgaatt tacaacagtc
     1681 aaaggtgtca aagaagatgt cgtcgacatc atgctgaacc ttaagaatgt cgtgttgacc
     1741 tgccacgccg acgagccggt tgaactacgg cttgaaaaga agggcgctgg cgtcgttaca
     1801 gctggtgaca tcaaggctac tggtgacatt gaagtcatca acaaagactt tgtcatctgc
     1861 actatcgacg atcctaagac tcaggttggc tttaacctgg ttgttgagaa cggtcgcggt
     1921 tatcgttcta tcgaagaatc ttcggttaag cgtctgcacg gcgacatgat cgctctagac
     1981 gccactttta gcccggttgt ccgtgtccgt tacaaagtcg aaaacactcg tgttggtcag
     2041 attaccgact tcgataagct actactgacc gtcgacactg atggttcgat cagccctacc
     2101 gacgctttcg aagaagccgc cgctatcctg cttaaccagt acactgcgct agccggttcg
     2161 actcgggtcg aagctgctcc agccgccaac gtagctgccc aagaagacgc tggtgaattg
     2221 atgactccga ttgaagatct gaacttgagt gcccgaacta ctaacgctct gatcaacaac
     2281 gacatccgta ctatcaatga cctagtcact cttagcgaca ccgaaatgcg tgaactaaag
     2341 ggctttggct ctaaagctat ggatgaggtc aaagataaac tggccgaaat ggagctttaa
     2401 tctatgcata gacacggata ccaaggtcga aaactgggtc gcgaacgcga tcagcgccga
     2461 gcactgatca aaggtctggc caccagctta gtcgagtacc agtcgattga gacgactcta
     2521 ccgaaggcta aagaagtggt gccatacctc gagaagctga tcaccaaggc taagaaaggt
     2581 gatctggcca atcgtcgagc cgttattgct ggtctcagca cccaagatgc tgctttcaag
     2641 ctagttgacg ctatcgcacc tcaactaagc ggccgagtca gtggccacct aaggatcgag
     2701 aagactcgtc tgcgggtagg cgataacgct cagatggctc gtgttagctt cgttgatgag
     2761 ctaaaagatg ctcctaagcc agctacaaaa gctacggcta agccgacccc taagaaacct
     2821 gctgctaaaa ctaccaagaa gccagccgct aaaacggagg ccaaataatg actaagaaaa
     2881 ccttttcact aaaagctagc gaagttaacc acgtttggac gctggttgac gccactgacg
     2941 cgccgctagg gcgactcagt accgttatcg ctcagcgcct aaccggcaag tacaaaccaa
     3001 ccttcacacc acacatggat gacggcgact acgtcgtagt tgtcaacgct gacaagctga
     3061 ttgtcaccgg tgacaaagaa gaggcaaaga cttattaccg gcacaccggt taccctggtg
     3121 gtattaagag ccgaagcttg aaagaggctc gtgctaaaga ctcgaccaag ctgatcgaag
     3181 cggccgtcaa aggcatgcta ccgcgcaata agctactagc cgaacggatg aaacgcctgc
     3241 gtgtgtttac tggcgaagaa cacggtcaca ccgctcagaa acctgttaaa ttggagatca
     3301 aataactatg gtcaaatcta attacttcta cggagtgggt cgacgcaaaa gtgctaccgc
     3361 acgggctcgt ctgaccggtg gtaagggtgt tattaccatt aacggccagg cggctgaagc
     3421 ttaccttgac ggtaaccaga cgctactggc tgagttaact gacccgttgg ctcttatagg
     3481 taagcaaaaa gaacttgatg taagcctggt tatctc
//
LOCUS       DASSBW010000032         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_926027, whole genome shotgun sequence.
ACCESSION   DASSBW010000032 DASSBW010000000
VERSION     DASSBW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926027"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..805)
                     /locus_tag="VFG56_00895"
     CDS             complement(116..805)
                     /locus_tag="VFG56_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET6622473.1"
                     /translation="MLIIGHRGSRGTLPENTVESLLEAIRVGVDILEFDVRITKDKQP
                     ILAHDLHMIKTHRKLDYYRRHSLANLRQQTKHSKYPATTLEQALEVSYGQVVVNIEIK
                     ESAAIPAVLDTVKKYVKKAADWDNVLFSSFSPLVLRKLRKAAPKACLALLHHRNPLNF
                     LVWYRSLDLTAVGFHRLYLSSFGLSVAKELGLFTYVYTVNRPQAAKKLLNRGVDGLVT
                     DYPADMIKSLN"
     gene            complement(838..1818)
                     /locus_tag="VFG56_00900"
     CDS             complement(838..1818)
                     /locus_tag="VFG56_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET6622474.1"
                     /translation="MARAPKLLLPEQYIDEAIKSINQAKSQVHILTMSIIDEPGSDRL
                     LDAARQAAKRGIDVSIAADTFTYTEPQKGSRVGDRLIAAQAMQRRLEKAGAKFHWLGR
                     TSFFAAAGRTHSKWCVVDDTVYSFGGVNLNNPGIVKWTDFMVKIDDANLAFFLRAEQE
                     RVIKADRAGHPYRSHLFGNDKDKVLIDGGFIGDSIIYRRSCYWAKRSKEVLLVSQYCP
                     TGRLSRLIKRVDRAQLYFNHWTNAGLLNRWVIRSGMLLSKHDTAYYRRPYLHAKFIIF
                     TLKDGKKVAITGSHNFVFGGVMLGTREVALETTDKRIIKQLEKFQTDHLA"
     gene            1909..2439
                     /locus_tag="VFG56_00905"
     CDS             1909..2439
                     /locus_tag="VFG56_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET6622475.1"
                     /translation="MADFNKVLEPGDFKNGTVNTVVEIPEGSMLKVEWDRERATFMLD
                     RVEPAIFAKPCNYGFIPQTLDEDGDELDTLIVCDEPLTTGIWLEAKILGVLKFEDDGE
                     VDDKVVVCPADDRNSGDRLNSLDDLGERWKQKIEHHFTHYKDLKKPGSTKVLGWGDAE
                     EAKKIIQESIDRWNDR"
     gene            2439..2867
                     /locus_tag="VFG56_00910"
     CDS             2439..2867
                     /locus_tag="VFG56_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6622476.1"
                     /translation="MLRETVAKALLINRDHQVLVLRIGEYKGKPERSHTPDLPGGLVD
                     PNESEHQAVIREIKEETGIEVNPNNLTLGYTETKAYPQENKSVTKMLYVGYLDGEFSV
                     TISWEHEAYEWCDTAKLLDRYKFRPFYEEAIGYLSDNKLL"
     gene            complement(2883..>3522)
                     /locus_tag="VFG56_00915"
     CDS             complement(2883..>3522)
                     /locus_tag="VFG56_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HET6622477.1"
                     /translation="LGIVHGLYKPLSNRIKDYIAMPKSNGYQSLHTTVRTPEDHIVEL
                     QIRTAQMHDYAERGLAASFHYNEQKLTDAYKKGQIAPMPASLSWIQELQQVAAKLSAG
                     ETASIDHLKLDLFTDRIFVYSPKGDIYDLPRGAYPLDYAYRVHSDVAAHACGFKIDGK
                     MVKFDEPLNDGDTVEIITKRSAKPNSDWLKWISTGHAREKLRAQLRKDGVRT"
BASE COUNT          862 a          780 c          967 g          913 t
ORIGIN      
        1 gaggtgtctt tgccgccggt gatgtaagga gtggcgccac tatgcaaatc gcttcggccg
       61 ccggagaagg tgccacggcg gcactcaata tgcgtgaatt tatcgaaggc cgcgactagt
      121 ttaacgactt gatcatgtca gccggatagt cggtgactaa gccgtccacg ccacgattta
      181 gcaacttttt ggcagcctgt ggtcggttca cggtatagac ataggtgaat aagccgagtt
      241 ctttggctac actcaagcca aaactcgata gatatagccg gtgaaaaccg accgccgtca
      301 aatcaagtga cctgtaccag actaaaaagt tgagcgggtt acgatgatgc aagagggcta
      361 aacaagcttt gggtgcggct ttacgcagtt tgcgtagaac gagtgggcta aacgacgaga
      421 acagaacatt gtcccagtcg gcagcttttt ttacatattt tttgacagta tctaggacgg
      481 ccggtatcgc agccgactct ttgatttcga tattgaccac tacctgtccg tagctgactt
      541 ccagcgcttg ttcgagagtg gtagccggat atttactatg tttggtttgt tgacgaaggt
      601 tagccaagct atggcgccgg tagtagtcga gcttacggtg agttttgatc atatgcaagt
      661 catgggctaa gatcggctgc ttgtctttag tgatgcggac atcaaattcc aagatgtcta
      721 cgccgacccg aatggcttcg agtagtgatt cgacagtatt ttccggtagg gtgccgcgtg
      781 atccgcggtg tccgattatg agcatgggct aattttatta caccgtccgg ttactggcta
      841 ggctagatga tccgtctgga acttctcgag ttgtttgata atgcgtttgt cggtcgtctc
      901 cagagcgact tcacgggtac cgagcatgac accgccgaag acgaaattgt gtgagccggt
      961 gatggcgacc tttttgccgt ctttgagagt aaatatgatg aacttggcgt gcaaataagg
     1021 ccgtcggtag taagcggtat cgtgcttact aagcagcatg ccgctcctga taacccagcg
     1081 gttgagcagg ccggcgttag tccaatgatt gaagtacagc tgggctctgt cgacgcgctt
     1141 aatcagtcga ctgagccgtc cggtcggaca gtattgcgat accagcaaaa cttccttgga
     1201 gcgtttagcc caatagcatg accgcctgta gatgatggaa tcaccgatga aaccgccatc
     1261 aatcaagacc ttatccttgt cgttgccaaa taagtggctg cggtaagggt ggccggctcg
     1321 atcggctttg ataacccgtt cttgctcggc gcggagaaag aaggctaggt tggcatcgtc
     1381 gatcttaacc ataaagtcgg tccatttgac aatccctggg ttatttaagt tgacaccccc
     1441 aaatgaatag acggtgtcat cgacgacaca ccacttgctg tgcgtacgac cggcggcagc
     1501 aaagaagctt gttcggccaa gccagtggaa cttagcgccg gctttctcta gtcgtcgctg
     1561 catagcttgg gcggcgatca aacggtcgcc gacccggctg cctttttgcg gttcagtata
     1621 ggtgaaggta tcggcggcaa tactgacatc gataccgcgc ttagcggcct gtctagcggc
     1681 atccagtaac cggtcagatc ccggttcgtc gataatcgac atggtcaaga tatgtacttg
     1741 cgatttggct tgattgatag atttgatagc ctcgtcgata tattgttcgg gtaacagtag
     1801 ttttggtgct cgagccatac cagacgatta tagcttaaat caccgttaaa gctggtataa
     1861 tttgaagtaa attatctaat atctaaaacg atctaaggag gctaataagt ggctgatttt
     1921 aacaaagtac tggaaccggg tgattttaag aatggtaccg tcaatacggt agttgagatc
     1981 ccggaaggat cgatgctgaa ggtcgaatgg gaccgtgaac gagccacttt catgctggac
     2041 cgggttgagc ctgctatctt tgctaaacct tgtaactacg gtttcatacc gcagacttta
     2101 gatgaggatg gcgacgaact ggatactttg atcgtctgcg atgagccgtt aactactgga
     2161 atatggctgg aggccaagat acttggcgtt ctcaaatttg aagacgatgg tgaagttgac
     2221 gacaaggttg tagtttgtcc ggccgatgat cgcaactccg gcgatcgttt aaacagctta
     2281 gacgacctgg gtgagcgttg gaagcagaaa atcgaacatc acttcactca ctacaaagat
     2341 ctcaagaagc cgggtagcac caaggtatta ggctggggtg atgccgaaga agctaagaaa
     2401 atcatccaag aatcgattga tcgctggaac gatcgctaat gttgcgcgag actgtcgcca
     2461 aagcactttt aatcaaccga gaccatcaag tactagtact gcgaataggc gagtacaaag
     2521 gtaagccgga acgatcgcac accccggact taccaggtgg tttagttgac cctaatgaat
     2581 ccgagcacca agcagtcata cgagagatta aagaagagac cggcattgag gttaatccaa
     2641 ataatttaac acttggctac accgagacca aagcctatcc gcaagagaat aagtcggtca
     2701 caaaaatgct ttacgtcggt tatttagacg gagagtttag tgtaactatt agctgggagc
     2761 atgaagctta tgagtggtgc gatacagcga aactattaga caggtataag ttcaggccat
     2821 tttatgaaga agctatcggc tatctctcag acaataagct gttgtagtaa gtttaatcat
     2881 cgctaagttc tgacgccgtc tttccttagt tgagcgcgta acttttctcg ggcatgaccg
     2941 gttgagatcc acttcagcca gtcgctattc ggcttagctg agcgtttggt gatgatttcg
     3001 acggtatcgc cgtcgttcag cggttcatca aacttgacca tcttgccgtc gattttgaaa
     3061 ccgcaagcgt gggcggcgac atcgctatgg acacggtagg cgtagtcgag cggataagcg
     3121 ccgcggggta ggtcgtagat gtcgcctttg ggcgagtaga caaatatccg atcggtgaac
     3181 aggtcgagct ttaagtggtc gatcgaggct gtttcaccgg cgcttaattt ggcggcgacc
     3241 tgttgcaatt cttgaatcca gctcaacgat gctggcatcg gcgcgatttg gcctttcttg
     3301 taggcgtcgg ttagtttttg ttcgttgtag tggaagctgg cggccagtcc tcgttcggcg
     3361 tagtcgtgca tttgagcggt tcgtatttgt agctcgacga tgtggtcttc cggtgtcctt
     3421 acggtggtat gtagcgattg gtagccattg gacttcggca tggcgatata gtccttgatt
     3481 cggttggaca aaggcttgta cagaccgtgg acgatgccga ga
//
LOCUS       DASSBW010000033         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_355132, whole genome shotgun sequence.
ACCESSION   DASSBW010000033 DASSBW010000000
VERSION     DASSBW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355132"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFG56_00920"
     CDS             complement(<1..298)
                     /locus_tag="VFG56_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622478.1"
                     /translation="MFALGHLTAWLDVFARSGGGGSSSGGGGGDGIIAFIGYLPMHFI
                     GAKLRKHGFNRDYWFVFQVVGWIIGILYALFWLVVWHVLGFLIGVAAIVGTGAGL"
     gene            complement(310..876)
                     /locus_tag="VFG56_00925"
     CDS             complement(310..876)
                     /locus_tag="VFG56_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET6622479.1"
                     /translation="MNTTLIVILVIVAVIAIFLWVMYNSLVTLKVRVNEAWSDITVQL
                     KRRADLIPNLVESVKGYASHEKQVFEDVTNARANVLNATNQGPAETAKAENQFEGALK
                     SLFAVSEAYPDLKASQNFQQLQAELVDTEDKIQASRRFYNGGVRDYNTKLQVFPNNVF
                     AGMFGFTKREFFEVDDMASVEKPVDVKF"
     gene            1011..1421
                     /locus_tag="VFG56_00930"
     CDS             1011..1421
                     /locus_tag="VFG56_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622480.1"
                     /translation="MREIGFGLMKLDLGQLIIALIVAWLGIWLLQGGRSSTVKAIIGA
                     FLAGLGLFAALMSIYVVDYRWVNTLLAIVVLIAIGVALFKAAGIATTAIGLVLILVGL
                     GAIDQLSLFGDIHLNGALKDTFDQAHRMWNRAVS"
     gene            complement(1526..2092)
                     /locus_tag="VFG56_00935"
     CDS             complement(1526..2092)
                     /locus_tag="VFG56_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622481.1"
                     /translation="MKQKPGRYTVIILALLAIIATVFAVVVTERGGGEAASSAAPESS
                     ETHEPRFLFGGATGWRKGPSNETSLALFGPNHDCFASVQYKDGSIAADKAERQKTDKY
                     FSDRGYVATTIDTAPMTMRTTDGQQNYELHQSAVATPAGADKVKGGQEFGYLQLSSDH
                     YLFVEGYCDTAEQLPTTIPALKAIKLNN"
     gene            complement(2259..2344)
                     /locus_tag="VFG56_00940"
     tRNA            complement(2259..2344)
                     /locus_tag="VFG56_00940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2308..2310),aa:Leu,seq:taa)
     gene            complement(2345..2418)
                     /locus_tag="VFG56_00945"
     tRNA            complement(2345..2418)
                     /locus_tag="VFG56_00945"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2384..2386),aa:Cys,seq:gca)
     gene            complement(2765..3499)
                     /locus_tag="VFG56_00950"
     CDS             complement(2765..3499)
                     /locus_tag="VFG56_00950"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6622482.1"
                     /translation="MRYGGLTQEQARFYRDQGYYRLLDCFDQTETAEMRDFISSEFNK
                     TQDRVDPSNPNRKLYGLYDRNPELMNRVLGKKALIGALTSILGPNVVFVKNRHNHATV
                     NNRYGSPAEGMHRDILQPTRGLVTAAVYLQDSTVGNGATRVIPGSHNLPYVGVPQADG
                     GGTWLAEHEEFAGLENQAVPVPMNEGDVLLFNGTLFHGVGYNPSGETRTSMTLGFRSV
                     DELDAQPDHSRQIVVAGDYIYRGNDR"
     gene            complement(3537..>3752)
                     /locus_tag="VFG56_00955"
     CDS             complement(3537..>3752)
                     /locus_tag="VFG56_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622483.1"
                     /translation="LKEQTWQACDGQTSLYKDALLDAWQRDGLYAYDNGTKFFRDIGT
                     VNSWLESQSDDALQRQLRYQQKLTHSA"
BASE COUNT          908 a          986 c          899 g          959 t
ORIGIN      
        1 acagacccgc gcctgtaccg actatggccg ccacgccgat caagaagcct agaacatgcc
       61 agaccaccag ccagaacaga gcatataaaa tcccgattat ccaaccaact acctgaaaga
      121 caaaccagta atcacgattg aaaccatgtt ttcgcagttt ggcaccgata aagtgcatcg
      181 gtaggtaacc gatgaaggcg atgataccat cgccaccacc gccgcccgat gatgagcctc
      241 cgccaccaga ccgggcgaat acatcaagcc aagccgtcag gtgaccgaga gcaaacactt
      301 acgatcaact tagaacttaa cgtctaccgg cttctctaca ctagccatgt cgtcaacttc
      361 gaagaactct ctcttggtga agccgaacat gccagcgaat acattgtttg ggaaaacctg
      421 aagcttggtg ttgtagtctc gaacaccgcc gttgtagaaa cgccgcgaag cttggatctt
      481 gtcttcagtg tcgactaact cagcctgaag ctgctggaag ttttggctag cctttaggtc
      541 cggataagct tcggatacgg caaacaggct ctttaaggca ccttcaaatt gattttcagc
      601 tttagctgtc tcggccggtc cttgattggt agcatttaaa acgttggctc gagcattagt
      661 cacatcttcg aatacttgct tttcgtgaga ggcgtaaccc ttgaccgatt caaccaaatt
      721 cgggatcaga tcggctcgtc gcttaagctg cacagtgatg tcactccagg cttcgttaac
      781 acggacttta agtgttacta agctgttgta catgacccac aagaatatgg ctataacggc
      841 aacgatgact aagattacta tcagggtagt gttcatttta tctccttttt atgatcacct
      901 cttattatag cggttttagc atcagcaaaa caaccgatat tgtaatttac cagaaaagtt
      961 ggttaaacta gttgaagtcg attatctccg gatgaggagg tgaagtacct atgagagaaa
     1021 tcggattcgg tctgatgaag ctggacctgg gtcagctcat cattgcgctg atcgtggctt
     1081 ggctaggcat atggctgctg caaggcggcc ggtctagcac cgtcaaggca atcatcggcg
     1141 ctttcttggc tggcctaggc ttgtttgctg cactgatgtc gatctacgta gtcgattacc
     1201 gctgggtcaa cacactcctg gccattgtcg tactcatcgc catcggtgtc gcactcttca
     1261 aagcagccgg catagcgacg accgccatcg gcctggtgtt gatactggtc ggtctcggtg
     1321 cgatcgacca gctgtcactc ttcggcgaca tccacctgaa tggtgccctg aaagatacat
     1381 tcgatcaggc acatcgcatg tggaaccgag ccgtcagttg accgctcgcg cattggccga
     1441 cacttctcat aacctaagcg gctcagcgta tctgactgtg atccttaacg gatcgctcca
     1501 aagtcagatg cgctgaccgc ttgttttaat tattgagctt gatggccttg agagccggaa
     1561 tggtagttgg cagttgctcg gcagtatcgc agtaaccctc gacaaaaaga taatgatcgc
     1621 ttgaaagctg gagataaccg aattcttgac cacccttcac cttgtcggct ccagcaggcg
     1681 tagccacggc tgactgatgt agttcgtaat tttgctggcc atcggtggtc cgcatggtca
     1741 tcggtgccgt atctatagta gtagccacat agccgcggtc agaaaagtac ttatcggtct
     1801 tctggcgctc agctttgtct gccgcaatgc tgccatcctt gtactgaacg gaagcgaagc
     1861 agtcatggtt gggaccgaag agtgccagcg atgtctcgtt tgacggccct ttcctccaac
     1921 ctgttgcacc gccgaataag aatcgaggct cgtgcgtctc acttgattcc ggtgcagcgg
     1981 atgacgccgc ttcaccaccg ccacgctcag tcacgaccac ggcaaataca gtggctatga
     2041 tagctagtag tgccagtatg atgacggtgt atcggccggg tttttgtttc ataactttag
     2101 taccactata caccagagca aggatgccgg gctgcactcg tagcggactt cgtccttttc
     2161 cgttccgccg tgctcagaat aaactattgt gcgcgtgctc tctctgccaa gtccccctgc
     2221 caccaaaaaa cgccctatcc ttcggacaga acgttttttg gtgggcgagg cgggacttga
     2281 acccgcacgt cttttggaca ttcgatttta agtcgaacgc gtataccaat tccgccactc
     2341 gccctggagg cacggaccgg atttgcaccg gcgtacacgg ttttgcagac cgctgcgtaa
     2401 ctactccgcc accgcgccgt ggctcccgga tattgtacca gatatccgta aagatggacc
     2461 tgctggcaac gacttttgct agttggctac atttttgggt actcaacaag tcttatacca
     2521 acatattgct aatgcttcaa ctttaatgta cgatacgaat acaataactt ttaaggaggg
     2581 actccatgga agtagttgga tggggatcgc cagttggttt gaccatcttc atggttggcc
     2641 taggtgtatc cgtatttcta ctgtgcctag ccgctcgcat ggttaacggc cggttcaaat
     2701 aaagatcgct tgagtgggaa gcgatctttt ttaaatatcc cgggcttcaa gctcgggata
     2761 tttattatct atcattgcct cgatatatat aatcgcccgc cacaactatt tgccgagaat
     2821 gatcaggctg agcatcgagc tcatcaacac ttctaaatcc cagcgtcatg ctcgtcctag
     2881 tctcgccgga tggattataa cccacaccgt ggaagagcgt gccattaaac agcagtacat
     2941 caccctcgtt cataggcacc ggtacagctt ggttttctag accggcgaac tcttcatgct
     3001 ctgccagcca ggtgccgcca ccatcggctt gcggtacacc gacgtaaggc aaattatgcg
     3061 agcctggtat gacgcgcgta gcgccattgc cgacggttga atcttgcaaa taaacagcgg
     3121 ctgtcaccaa gccgcgcgta ggctgaagga tatcacgatg cataccctct gctggcgagc
     3181 catacctatt attgacagtg gcgtggttgt ggcgattttt aacaaacacc acgttaggcc
     3241 caagtataga ggttaacgca ccgatcaacg ccttcttacc caaaactcga ttcatgagct
     3301 caggattgcg gtcatatagc ccatacaact tacggtttgg gttagaagga tctaccctgt
     3361 cctgagtttt gttgaattca gaactaatga agtcgcgcat ttcagcggtc tcagtctgat
     3421 caaaacagtc cagcaggcgg tagtaacctt ggtcacgata aaatcgagcc tgctcctgag
     3481 tcagtccacc gtatctcata aatatatttt actataatta taggtaaaaa tcaatactag
     3541 gcactgtgcg tcagtttctg ctggtatcgc aactgccgct gcaaagcatc atccgactgc
     3601 gactcaagcc agctgttcac cgtcccgatg tctctgaaaa actttgtacc gttatcgtaa
     3661 gcgtatagac cgtcccgttg ccaagcatcc aggagagcgt ctttgtaaag tgaagtttgg
     3721 ccatcgcatg cctgccaggt ttgctcctta ag
//
LOCUS       DASSBW010000034         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1326808, whole genome shotgun sequence.
ACCESSION   DASSBW010000034 DASSBW010000000
VERSION     DASSBW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326808"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFG56_00960"
     CDS             complement(<1..538)
                     /locus_tag="VFG56_00960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6622484.1"
                     /translation="MTDTITIAVLAGTTRAKRESIKAARYVAEEGRKLDGVDIVFVDP
                     KDFDLPGDGNDPEGKDPRYSAIVDRADAFFIVTPEYNHSFPGSLKRMLDSELELYNHK
                     PVAMAGVSDGNWGGVRAVESLVTAIRETGLVVMSWDIYFPYVQDIFDDDGAIKPEHRA
                     RYQTNLAKLYAELLWFARL"
     gene            complement(672..920)
                     /locus_tag="VFG56_00965"
     CDS             complement(672..920)
                     /locus_tag="VFG56_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622485.1"
                     /translation="MTSTINKQVNITALGFKKNLTAYPRQMEFEGTTYDFVDAGLSCV
                     IRHGERLSQILTLSDGYRQFRLRSDNHGGAWTLLSMTV"
     gene            1191..1829
                     /locus_tag="VFG56_00970"
     CDS             1191..1829
                     /locus_tag="VFG56_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6622486.1"
                     /translation="MQIVIDDQLAEAADKHLKASDPVLRPIIERAGPCNIRPHRNYYW
                     ELVDSIISQQLSVKAAATIERRFQELFDSEVPTPEQILTSSVENLRTVGLSRPKAGYI
                     LDLAEHVSSGKLKFDKFDDMSDEAIAKVLIDVKGIGQWTADMFLMFCMARPNVLPVGD
                     LGIRNSIRSLYSFDHLPDATDIEAIAERYDWAPYRSIASWYIWQNLDNTPAL"
     gene            complement(1826..1963)
                     /locus_tag="VFG56_00975"
     CDS             complement(1826..1963)
                     /locus_tag="VFG56_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622487.1"
                     /translation="MSKNTQAIIILVVALLIMAALGVLAWRVLISDPDSVQKLVEDNV
                     R"
     gene            complement(2003..>2323)
                     /locus_tag="VFG56_00980"
     CDS             complement(2003..>2323)
                     /locus_tag="VFG56_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET6622488.1"
                     /translation="RDRLAQKILAENIQSLGVGNQAKLIKSSVSSWSDTTKEEEFDLI
                     FADPPYHDLQLSTVAKLVDYLNPNGLMILSFPGRESAPTVNGVVVVDNRSYGDAALAF
                     YRLD"
BASE COUNT          602 a          583 c          553 g          585 t
ORIGIN      
        1 acaacctagc gaaccaaagc agctcggcgt aaagcttggc tagattggtc tggtaacgcg
       61 cccggtgttc cggtttgatg gcaccgtcat cgtcaaagat atcttggaca tacgggaagt
      121 agatgtccca gctcatcacc accaggccgg tctcacgaat agccgtcacc agagattcga
      181 cagccctaac gccgccccag ttaccgtcac ttacgccagc catagcgacc ggtttgtggt
      241 tgtagagttc cagctcgctg tcgagcatac gcttcaagct accaggaaag ctgtggttat
      301 actccggcgt gactatgaaa aatgcgtcgg cccgatcaac gatggcgctg taacgtgggt
      361 ccttgccctc tggatcattg ccatcacccg gcagatcaaa atctttcgga tcgacaaaca
      421 ctatgtcgac gccgtctagt ttacgacctt cttctgccac gtaacgggcg gctttgattg
      481 actcacgctt agcccgtgtc gtaccggcca atacggcaat cgttatggta tctgtcatat
      541 ttgttccttg gttactggcc caagtatagc aaagcccgcc ctctgtatgg cgacgagcta
      601 tgccctagag gagcaagcca cacaaaaaca gcgccctata ttcagagcgc tgtcttcgtg
      661 tggtgataag tctagacagt catcgaaaga agcgtccagg ccccaccgtg gttatcacta
      721 cgcagcctaa actgcctata tccgtctgac aatgtcagga tctggctgag ccgttcgccg
      781 tggcgaatga cacaactcag gccggcatcg acgaagtcgt aggtagtacc ttcgaactcc
      841 atctgcctag gataagccgt caggttcttc ttgaaaccga gggctgtgat gtttacctgt
      901 ttgttaatag tacttgtcat attcactcct aacttttgga tgagaccaaa aggctaattc
      961 tgttattgat tagcgaacgg gttttggagt gagctaatct cggataggga caatcaaccg
     1021 ttcaaatgtc ctgtttaccg acctagcttt ttaatatgta gcgtcttttt taggagacac
     1081 taagctgatt taagcttgcc ggtggaaagg tatgaaccag taccgacata gctatggtta
     1141 attatacacc gcttacgttt atgtcgacta gacaagctac aatagacctt atgcaaatag
     1201 tcatcgacga tcagttagcc gaagcggccg ataaacacct gaaggctagt gacccggtct
     1261 taagaccgat catagaacga gccggaccgt gtaacattcg gccacatcgt aattactact
     1321 gggaactggt tgattcgatc atctcgcagc aacttagtgt caaagccgct gctaccatcg
     1381 agcgtcgttt tcaagagttg ttcgattctg aagtgccgac accagagcag atcctgacca
     1441 gttcggtcga gaacttaagg actgtaggat tatcacgacc aaaagccggc tacatcctcg
     1501 atctagccga acacgtaagc agcggtaagc taaaatttga taagttcgat gatatgagcg
     1561 acgaagctat cgctaaagtc ctgatcgacg taaaaggtat cggtcagtgg acggccgaca
     1621 tgttcttgat gttttgcatg gccaggccca acgttttacc ggtcggagac ttaggtatca
     1681 gaaacagtat ccggtcactc tatagctttg atcacttacc cgatgctacc gacatagaag
     1741 ctatagccga aagatacgac tgggcaccgt atcgttcgat cgccagttgg tatatctggc
     1801 aaaacttaga caatacaccg gcgctttagc gaacgttatc ttcgacaagc ttttggaccg
     1861 aatccgggtc gcttataaga acccgccagg ccaagacacc cagggcggcc atgatgagga
     1921 gagcgacaac tagtatgata atggcttggg tatttttgct cataaatttc agacttcgta
     1981 ttaaaaaaga cctcttataa ggtcagtcta ggcggtagaa agccagcgcc gcgtctccgt
     2041 aactacgatt gtccaccaca acaactccat taacggtcgg cgcagactcc ctacctggaa
     2101 atgataatat cataagcccg ttgggattca aataatcaac tagcttggcg actgtggata
     2161 actgcagatc gtgatacggc ggatcggcaa atatcagatc gaattcctcc tctttggtgg
     2221 tatcactcca gctgctgacg cttgatttga tcaatttggc ttggttaccc acaccgagtg
     2281 attggatatt ttctgccaag attttttgag ctaacctatc gcg
//
LOCUS       DASSBW010000035         5029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1517754, whole genome shotgun sequence.
ACCESSION   DASSBW010000035 DASSBW010000000
VERSION     DASSBW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517754"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1477
                     /locus_tag="VFG56_00985"
     CDS             230..1477
                     /locus_tag="VFG56_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6622489.1"
                     /translation="MIRHILNSRRRIKKREAKLIALLGLPTFGLALSISAVVTYAPLL
                     AHQYISSAVIIGLIIGTEGLMALFLPVIIGHWSDNLRTRLGGRLPFVLIGGPLSALAL
                     VCIGLSNSLALLIVSIAVFFFAYYVAYEPYRALYPDLISKRATSRAQGVQALWRGAGT
                     GLALIIGGPLIVVALWLPFVMAAFLVLVSIACFLWFLLKQNGVPKQSRHKNEPLVEVF
                     KGLWRLLIEHGQIRNYIIANMLWELSLAALKTFIILYLTLGMGFSTGMAALLIALVAV
                     FIGIGSPISGILADHFGRVKVMTVAVMIFGVSLLLPSITDIKWLLAIALPVGGLSGAI
                     VMTLPYALLIPMMPSRKHGRLTGLYSTSRGVGIMLGPILAGAAISIAPKTYLAHGFAA
                     MWLVCGGAMLLSLLFLRQLDIDS"
     gene            1541..2341
                     /locus_tag="VFG56_00990"
     CDS             1541..2341
                     /locus_tag="VFG56_00990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6622490.1"
                     /translation="MSLKSIKIHRPQGRSQSRAAAKEKATAALYWLIFLSMALMPAHW
                     YIPEQLVPLEDMVSTQQAWQLYLSTMTSPVAIWILPLGVVINLGLAYAVKKINSSRGW
                     LNWSLSKTGSNTAQVAAEARLSGLLYLALFAYCLPFFVNFEEHIFRVYENPLVSWVMG
                     SLVFGLAHLLSKARLKIVYLQILVGLVLVIVYQQAGLWPLIAVHTTANLTALTRMAYH
                     MQLKPRFKHLAMSSAYRRQVHSLAARRSVRRHLPTVLGWLERPLDAEA"
     gene            2448..3620
                     /locus_tag="VFG56_00995"
     CDS             2448..3620
                     /locus_tag="VFG56_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622491.1"
                     /translation="MKPERMGSPSLFSVEQERTMRADMRTIPDDYVPEVLPPSGEKLS
                     VVVNKDHAAQVLDRLMAAYENDLPPYNDHERVRVPHDPRFMPASMPRGGIEHATFLFN
                     TCYYMRGGIKSNEAVDRLARLYDQRPELFIAEIAAKAQPDDIISALKQSGLGFQETVA
                     GQWIENSRRLTERYGGDPRRIFDGIDTYEASLGVVQNDRRGQGFVGFQEKMTSMIIYY
                     LMDDGLIEPFKFPIPIDLHVMRVSIANQMITFPDAPNGTNLFTDETLAALRELYYDYA
                     VERGVDPLRLCDAVWMLSESSCGRHPGNATLEPLGRTRRNGRKTYLIPGVVDPSSPVQ
                     QSAYASACGICPIEETCQFNIPGTHYYVGGSIIIRGKRMRFPISQFMAQSHQEYLF"
     gene            complement(3612..4136)
                     /gene="msrA"
                     /locus_tag="VFG56_01000"
     CDS             complement(3612..4136)
                     /gene="msrA"
                     /locus_tag="VFG56_01000"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6622492.1"
                     /translation="MVSKIVLGGGCFWCLEAAYQMVRGVIEITSGYAGGQTIEPNYYQ
                     VCSGTTGHAEVVAVEFDPKVISLKQVLDVFWTIHDPTTKDAQGADIGNQYRSVILYSE
                     ESQLPVIEKSLAAAQALFDRPIVTEIQPLERFYQAEAEHQDYFKNHPEQAYCQIVINP
                     KLSKLRTTLQGLLK"
     gene            complement(4129..4521)
                     /gene="msrB"
                     /locus_tag="VFG56_01005"
     CDS             complement(4129..4521)
                     /gene="msrB"
                     /locus_tag="VFG56_01005"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET6622493.1"
                     /translation="MKAEDELRDKLGPAAYDVMRGRGTEAPFSGALLNEHRDGSFSCR
                     VCGSVIFKSDSKFDSGSGWPSFSEPANQAAVKLSTDKSHGMLRTEVSCASCGSHLGHV
                     FDDGPVERGGQRYCVNSVCFDFKPNHNG"
     gene            4592..>5029
                     /locus_tag="VFG56_01010"
     CDS             4592..>5029
                     /locus_tag="VFG56_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET6622494.1"
                     /translation="MVAKADKKHDSEVLARRALQVQRGAARAAVLGVNDGLVSVLCIV
                     LGVAGAGASQSAVLLAGFAGLIAGAVSMAAGEWISVRSQVELFGGILKDLKRLMKQDR
                     PLLVEQVADNLSASGHGDKTARTAAKEIARSDKHLAEGYARQVM"
BASE COUNT         1141 a         1225 c         1317 g         1346 t
ORIGIN      
        1 gtgactcggt cttcggggtt ttggtttgag gggctggtgt gttgaccggt tgagtgatgg
       61 attgctggct gatctgccac caaatcacac cgagagtcat aatcaagccg attatgatga
      121 gcccaccgta gatgtagctg cgttttagcg tcatacacct cctttgtaac tattatactg
      181 cggtctgagt ttaagcaaaa tcgctgacta acgagtacaa tgagttggaa tgatccgtca
      241 catcttaaat agccgtcgtc gcatcaaaaa gcgcgaagct aagctaatcg ctctcctagg
      301 tctaccgact ttcggtttgg ctttatcgat tagtgctgtc gttacatacg cacctcttct
      361 agcgcaccag tacataagct cggcagtcat cataggtttg attatcggta cggaaggtct
      421 gatggctctg tttctaccgg tcataatcgg ccactggtcg gataatctac gaaccaggct
      481 gggcggacgg ctaccgttcg ttttgattgg tggccccctg tcggctttag cgctggtttg
      541 tatcggcctg tcgaattctc tagccctact catagttagt atcgccgtat tcttctttgc
      601 ctactatgtc gcatacgagc cgtatcgagc gttatatccg gatctaattt ccaagagagc
      661 tacttcgcgt gcccaaggcg tgcaagcgct atggcgtggt gccgggacgg gcttagctct
      721 catcataggc ggacctttga ttgtggtggc gctatggctt ccctttgtta tggcggcgtt
      781 cttagtcctc gtgtctatcg cttgtttctt gtggttcttg ctcaagcaaa acggcgtacc
      841 gaaacagtcg cgtcacaaaa atgaaccact ggtcgaagtc ttcaagggat tgtggcgact
      901 gttgatcgaa cacgggcaga tccgcaatta catcatcgct aacatgctgt gggaattgtc
      961 gctggcggcc ctcaaaacct ttatcattct ttatttgact ctgggtatgg gcttctcgac
     1021 cggcatggcc gcgctcctta tcgctctggt tgcggtattt ataggcatcg gctcaccgat
     1081 cagcggtatc ttggccgacc acttcggccg agtcaaggtg atgaccgtag ccgtgatgat
     1141 tttcggcgtt agcttactat tgccgtcgat aaccgatata aagtggctgt tagctattgc
     1201 ccttccggtc ggaggtttat cgggcgcaat agtcatgaca ctgccatatg ccttgcttat
     1261 tccgatgatg ccgagccgta agcatggccg gttaactggt ctctattcga catcgcgcgg
     1321 tgtcggtatc atgcttggtc ctatcttggc cggagcagct atatcgatag caccgaagac
     1381 ttatttggcg catggctttg ctgccatgtg gctggtttgt ggtggtgcca tgctactgag
     1441 cctgctcttc cttcggcaac ttgatattga cagctagatg attttttagc atattaagtt
     1501 ttagtccgtt gtccctatag tggccggagg tgaactcaag atgagtctta aaagtataaa
     1561 aattcatcga ccccaaggcc ggtcacaatc acgagctgcc gccaaagaaa aggctacagc
     1621 cgcattgtac tggctgatat ttctgagtat ggcgctgatg cctgctcatt ggtatatacc
     1681 cgagcagttg gtgccgctcg aggatatggt gtctacacag caggcgtggc agctttacct
     1741 gtcaacgatg accagtccgg tagctatctg gatactgcct ttaggcgtgg tgattaatct
     1801 tggcttggcc tacgcggtca agaagatcaa cagtagcagg ggctggctta actggtcgct
     1861 gtctaaaaca ggctccaaca ccgctcaggt ggcagccgag gctaggttga gtggtttact
     1921 ctaccttgcg ctttttgcct actgcctgcc tttttttgta aatttcgagg agcacatctt
     1981 tcgagtttac gaaaatccgc tggtgagctg ggttatgggt agcttagttt ttggtttggc
     2041 tcacttgttg tccaaggcca gactcaagat cgtctatctt cagatattgg tcggtttggt
     2101 tttggttatc gtgtatcagc aagccggttt gtggccactg attgccgttc ataccactgc
     2161 caacctgacc gccctgacgc gcatggctta tcacatgcaa ctcaaacccc ggttcaaaca
     2221 tctggctatg agttccgctt accgccggca ggtacatagc ttggcggcca ggcgaagcgt
     2281 tagacgacac cttccgacag tacttggctg gcttgagcgt ccacttgacg ccgaagcctg
     2341 acaacggact tacccgccga gcaatcggcg ggtttgtcac gtcgcaatga aattatcaat
     2401 catccttatg gttgcttgag agttggcaat cggtattaaa taaatcaatg aaacctgaac
     2461 ggatgggttc accgtcgcta tttagcgttg agcaagaacg gaccatgcgt gccgacatga
     2521 ggaccatacc tgatgattac gtaccagaag tattgccgcc aagtggcgag aaactatcgg
     2581 tcgtggtcaa taaagatcat gccgcacaag tactcgatag gcttatggcg gcttacgaaa
     2641 atgatttgcc gccctataac gatcatgagc gtgttcgagt accacatgac cctagattca
     2701 tgccggctag tatgccgcgc ggtggtatcg aacacgccac ctttttgttt aacacttgct
     2761 actacatgcg cggaggtatc aaatcaaatg aagcggttga tcgcctggcg cgtctatacg
     2821 atcaacgacc ggagttgttt atcgcagaaa tcgccgccaa agcccagcca gacgatatca
     2881 tctcagcctt gaagcaaagt ggtctcggtt ttcaggaaac agttgcgggt caatggatag
     2941 aaaactcacg tcgtttgacc gagcgctatg gtggtgatcc gcggcgaata tttgatggta
     3001 tcgatactta tgaagccagc ctcggtgtcg tccagaatga ccggcgtggc caaggttttg
     3061 ttggctttca ggaaaaaatg accagcatga taatttacta tctgatggat gacggactga
     3121 tcgagccgtt taagtttcct atcccgatcg atctgcatgt catgcgggtc agtatcgcta
     3181 accaaatgat aacttttccc gacgcgccta acggcaccaa tctttttacc gacgagactc
     3241 tagcggcatt gcgtgaactc tactatgact atgcagtaga acggggtgtc gatccgctgc
     3301 gtctatgtga cgccgtttgg atgctgtcgg aatcttcttg tggtcggcat cctggcaacg
     3361 cgacgcttga acctctcggc cgcactagac gcaatggtcg caaaacctac ttgatacccg
     3421 gagtggttga tccgtctagt ccggttcaac aatcagctta tgcttcggct tgcggcatct
     3481 gcccgatcga agaaacttgt cagtttaaca ttcccggcac gcactactat gttggcggca
     3541 gtatcataat caggggcaag cggatgcgat tcccgatcag ccagttcatg gcccagtcgc
     3601 accaggaata tttattttag caagccttgt agagtagttc ttagcttgga caatttaggg
     3661 ttgataacta tttgacaata cgcctgctcg gggtggttct tgaagtagtc ttgatgttct
     3721 gcttcggcct ggtaaaaccg ttcaagtggt tgaatctcgg taacgattgg tcggtcgaat
     3781 aacgcttgtg cggctgccag agatttttca ataacaggta gttgggactc ttcgctataa
     3841 aggatgactg agcggtattg attgccgata tcagcacctt gagcatcttt agtggtcgga
     3901 tcgtggatag tccaaaagac atcaaggact tgtttcaaac tgatcacctt agggtcgaac
     3961 tctactgcaa cgacttcggc gtgtccggtg gtgcctgaac aaacctgata ataattaggt
     4021 tctatggttt gaccaccggc ataaccagaa gttatctcaa tgacacctcg aaccatctga
     4081 tatgccgcct ctaggcacca gaagcaaccg ccgcctagga ctatcttact aaccattgtg
     4141 atttggctta aagtcgaaac agaccgagtt gacgcaataa cgctgaccac ctctttcaac
     4201 cggtccatca tcaaaaacat gaccaagatg tgagccacag ctggcacacg acacttcggt
     4261 tctcagcatg ccgtgggatt tgtcggtaga cagcttgacg gcggcttgat ttgccggctc
     4321 gctgaaactc ggccaaccag agcctgagtc gaatttagaa tcagatttga agattacact
     4381 gccgcagact cggcagctaa aactaccgtc ccggtgctcg tttaggagtg cgccgctaaa
     4441 tggcgcttca gtacccctgc ctcgcatgac gtcgtaagca gctggaccta gtttgtcgcg
     4501 gagttcgtct tcagctttca tacctctatt atatactgcc cgtagctctg cgatgcgtta
     4561 caatggttga ttgaaataag cgaggagcga tatggtagcg aaagccgata agaaacacga
     4621 tagcgaagtt ctagcccgcc gggccctgca agtccagcga ggcgccgctc gcgcggcagt
     4681 actaggagtt aatgatgggc tggttagcgt actatgtata gtcttaggtg tcgccggagc
     4741 tggcgccagt cagagcgccg tattgctggc gggctttgcc gggctgattg ctggtgctgt
     4801 cagtatggca gcgggtgaat ggatctcggt aagaagccaa gtcgaactgt tcggtggtat
     4861 cttaaaggat ctcaagcgtt taatgaaaca agaccgacca ctgttggttg agcaggtcgc
     4921 tgataactta tcggctagtg ggcatggcga taaaaccgct cgcacggcag ccaaggagat
     4981 cgctcgtagt gacaaacact tagccgaagg ctatgcccgt caggttatg
//
LOCUS       DASSBW010000036         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_107979, whole genome shotgun sequence.
ACCESSION   DASSBW010000036 DASSBW010000000
VERSION     DASSBW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107979"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFG56_01015"
     CDS             <1..276
                     /locus_tag="VFG56_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET6622495.1"
                     /translation="LGSPWIAAGSSFALFTLGALAPLIAWFFTGGATAIWWAIGLTGL
                     GSLIVGGYIGRSSGRSLAYGALRQLAIVVLASGVTYGIGHLFGTAVS"
     gene            301..1074
                     /locus_tag="VFG56_01020"
     CDS             301..1074
                     /locus_tag="VFG56_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6622496.1"
                     /translation="MGRIAKQKRFFKTVHHYVWHDLAIALVILLPLVAAVYALISSRT
                     FQLGGDLVRSVETSQKVVALTFDDGPEPPYSDQLLSILKDKGVKATFFLIGQEMQRHP
                     AATQRLIDSGNQIGNHTLTHDGLAFVSPSYIAWQIESTDRIIRNHGYDGTIPFRPPYG
                     MKLIWLPQYLQQHDRPNIMWTVVADDNNAAQATRQIRHRVLSQLKPGMIIDMHAMYAH
                     NKPVRDVLPRLIDDIKAAGYRFVTVDQLLTYSHGHKSND"
     gene            1408..>2183
                     /locus_tag="VFG56_01025"
     CDS             1408..>2183
                     /locus_tag="VFG56_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622497.1"
                     /translation="MPTSTEKLGPAGGPPVRVGPGNHPPLSRAQRREAGRNKSGSRKA
                     AATTPSTVGKAEPSTQSDRPVPKMGFPAFWYGFKKLCKFWGSPPVVPNLSRKERDLLS
                     QAYDEALVEEAQRQEAKRRVEVRERSVDEIRSLTDQLPKLVVAFFNTKGHASTTTTTV
                     HVAATTAEFTRTVVTLVDGNPAEGTCAARLGRNAGETVTTQQLARTISSDEDLRHRDF
                     VTEITRARPSKQGVRLVSADSIVDETSRLTGKDMDRILELL"
BASE COUNT          522 a          555 c          579 g          527 t
ORIGIN      
        1 ttaggctcac cttggattgc ggccggttca tcatttgctt tgttcactct cggtgcttta
       61 gcgccgctaa tagcctggtt ctttactggt ggggcaacag ctatctggtg ggcaattggt
      121 ctaactggtc taggcagctt aattgtcggt ggttacatcg gtcgttctag tggccgaagt
      181 ttggcttatg gcgccttacg tcagttagct atagtagtgt tagcttcggg tgtgacttat
      241 ggcatcggtc acttattcgg taccgccgtc agttagacgc ttgtgctatg atgtcggcag
      301 atgggccgaa tagctaaaca aaaacgattc tttaaaacag ttcatcatta cgtctggcat
      361 gacttggcga tagccttggt tatcctacta ccattagttg cagcagttta tgcactgata
      421 tcctcgagga cattccagct aggcggtgac ttggttcggt cggttgaaac tagccaaaaa
      481 gttgtggcgc tgacttttga tgacggcccc gagcctccct actcggatca gttgttaagt
      541 atcttgaaag acaaaggtgt taaggctacc ttctttttga ttggccaaga gatgcagcgt
      601 catccggcgg ctacccagcg tctaatcgac tccggcaatc aaatcggcaa ccacaccctg
      661 acgcatgacg gactagcctt tgttagtccg agctacatcg cttggcagat tgagtcgact
      721 gatcgaatca ttcgcaatca cggctacgat ggcactatac cgtttcgacc accatacggg
      781 atgaagctta tctggctacc gcagtatttg cagcagcacg accgcccaaa tattatgtgg
      841 acggtagtcg ccgacgataa caacgccgcc caggctactc gacagatcag acatcgggtt
      901 cttagtcagc tcaagccggg tatgattatc gatatgcacg ccatgtacgc tcataacaaa
      961 ccggttcgag acgttctgcc ccggcttatc gatgacatca aagcagccgg ttatcgtttc
     1021 gtgacggtcg accagctact tacttatagc catggccaca agtcaaacga ctaaattaca
     1081 cttaccgcac tataatgttg tagaatagtc cattacctgt taagatttgc caaaatctcg
     1141 tgaaagttgt atataattaa gctaaagtca aaacgtcaaa acagagatca gtgattgttg
     1201 tacgatcaaa gctcttacca gacgtcaggt gggtcgggtt agttttctta agggtttcga
     1261 ccggacaaaa atccgggctt ttagattatc ttctaaaagt acagaccgct agtaggcagt
     1321 gctaatgcag gcctcgcggt ctgaaacaag cactttaaaa agcagatttg tttacatccg
     1381 aactaatcca agagatgagg tggcaaaatg ccaaccagca ctgaaaagct tggtccggcc
     1441 ggcggtccgc ccgtcagggt gggacccggc aatcacccac cgcttagccg agcgcaaagg
     1501 cgcgaagctg ggcgcaacaa gagtggtagc cgcaaggctg ccgctaccac gccttcgact
     1561 gtcggcaagg cagagccatc gactcagtcc gaccgaccgg taccgaagat gggtttcccg
     1621 gctttctggt acggcttcaa gaagctgtgc aagttctggg gatcgccacc ggtagtaccg
     1681 aacttgagtc gcaaggaacg tgatcttctg tcgcaggctt acgatgaagc gctggtcgaa
     1741 gaggcccagc gacaagaagc caagcgtcgc gtggaggttc gcgaacggtc ggtcgatgaa
     1801 attcgcagcc tgaccgatca attgccgaaa ttggttgtag ccttcttcaa caccaagggg
     1861 catgcttcaa ccacgactac gaccgtccac gtggcggcga ctaccgcgga gtttactcgg
     1921 acggtagtaa ctttggtcga cggtaacccg gctgaaggta cttgtgccgc tcggttgggt
     1981 cgtaacgccg gcgagacggt gacgacccag cagttggctc gtaccatcag tagcgacgag
     2041 gacttgagac accgcgattt cgtcactgag atcacgcggg cccgtccctc caagcagggt
     2101 gttcgtctgg ttagtgccga ctctattgtc gacgagacca gccgactgac tggcaaggat
     2161 atggacagga tcctcgagct gct
//
LOCUS       DASSBW010000037         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1411386, whole genome shotgun sequence.
ACCESSION   DASSBW010000037 DASSBW010000000
VERSION     DASSBW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411386"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFG56_01030"
     CDS             complement(<1..956)
                     /locus_tag="VFG56_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET6622498.1"
                     /translation="MMARVTSAASYGFDGKLIEVECDSTKGLPAFQIVGLGDKAITEA
                     RERVKSAITNSHLNYPAKRLTVNLAPANLPKDGAHFDLPIALAILIASGQLKQSAVDG
                     ILSAGELSLSGELRPIRGIINITETAKQSGLSTVIVPAGNAAQAAMVKGVTVLAANNL
                     AEVYLHLVGEKRLPVVEASSSPKAPRSTVDLADIRGQQTAKRALVIAAAGHHNLLLDG
                     PPGAGKTMLARVLISLLPEPTYDERLEITKLHSLSGESLDEIVAERPFRSPHHTSSTI
                     SLIGGGAKPQPGEVSLAHHGVMFMDEIPEYSRSSLEALRQPL"
     gene            1175..1560
                     /gene="ssrA"
                     /locus_tag="VFG56_01035"
     tmRNA           1175..1560
                     /gene="ssrA"
                     /locus_tag="VFG56_01035"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1659..2654
                     /locus_tag="VFG56_01040"
     CDS             1659..2654
                     /locus_tag="VFG56_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET6622499.1"
                     /translation="MEQVKGVNLGGWLVLERWISPSVFSGTKARDEYSLCSELGAKAA
                     GRLKIHRDNFITEDHIKELSRQGLNTLRLPVGYWLFGSESPFVDGADAYVDRVFSWVD
                     KYDFKVILDFHAAPGSQNGNDHSGQAGEINWSEPDNVLKSLEFIDKLTRRYGKEEGLV
                     GVEVLNEPDWGIGLPTLIDYYAKADEIVKSNCHPGVSTIVHDAFRPKEMALALDAAGL
                     DVVLDVHLYQLFTPEDRTLNLKGHIRKTKRDWKKLLKKLGRSRQLLVGEWSVAMHELY
                     RKIDQPDCHYTPGDYRKYFLAQCQVFEKTKAGWTYWTARTEDGGIWSYIDQDFEV"
BASE COUNT          663 a          616 c          728 g          655 t
ORIGIN      
        1 tctagcggtt ggcgtagggc ctctaagctg gagcgagagt actcaggtat ctcgtccatg
       61 aacattacac cgtgatgggc tagtgacact tcgccgggtt gcggtttggc accacctcct
      121 atgagagaaa tggtgctaga ggtgtgatga ggagaacgaa agggacgttc agcaactatc
      181 tcgtctaaac tttcgccgct aaggctatgg agcttagtga tctctagccg ctcatcgtag
      241 gtgggctcag gcagtagaga gattagtacg cgcgctagca tcgtcttacc agcgcccggc
      301 ggtccgtcga gcagtaggtt atgatgaccg gcggcggcga taactaaggc ccgcttagcc
      361 gtctgctgac cccgaatgtc ggccaagtct acggttgatc gaggagcttt aggtgacgat
      421 gatgcctcga caaccggtaa tcgtttttca cctaccagat gtaagtacac ttcagctagg
      481 ttgttggccg caagtacggt gacgcctttg accatggcgg cttgagcggc attacccgcc
      541 ggaactataa cggtactcaa gccgctttgt ttagcggttt cagtgatgtt aataatgcca
      601 cgaataggtc ttaattcacc actgagggag agttcaccgg ccgacaagat gccatcgact
      661 gccgattgct tgagctggcc tgaggcgatc agtatagcca aagcgatagg taggtcgaag
      721 tgagccccgt ctttgggtaa gttagccgga gccagattga cggtcagacg cttagccggg
      781 tagttgagat gcgagttggt gatggcgctt ttgactcgtt cgcgagcttc ggtgatagct
      841 ttgtcgccaa gaccgacgat ctggaaagcc ggtaggcctt tggtggagtc gcactccacc
      901 tcgattagct tgccgtcaaa cccgtagctg gcggccgatg taactctggc catcataatg
      961 atgtcattta aacacttata tcggccataa gcaattccac ccctggtatg tcatggtaaa
     1021 atttgctata ataatgattg tagccaatgt ggctatccgg acgtttaaac caaggcgctt
     1081 cagcgactgt ttaaacggac gaaaggagga atatagtctt aagccaaagt ttttgctgct
     1141 aagcaaaacg aggtagagac gtcaattccg acgtggggct gacatagctt tcgacggatg
     1201 gaccttaaca gatcgcttgg caagtcgaat tctggacgta aaccagttat ttaactgcaa
     1261 actttgttgc aaaagtaaaa gctgctttcg ctatgcctaa gctagctttt gcgccagttg
     1321 tagcttaatt taagctgcga ccatcccgcc gctgacgtct gataagcgat agcggggtgt
     1381 catatttatc agacttggcg ttgacgtagc cgtagggcgt ccgcgcgaca tttacctatg
     1441 gaacgaggtt gttatttttg tcgttaatcg agacaactcc gtaatttaaa agcgactaag
     1501 cttgtagatg ggcggttagt taccattcgg accggggggc agtaccccgc agctccacca
     1561 aactaaaaca gctgaccctc gacggtcggc ttttttagtt tcttgaaata ttcggggtct
     1621 gccctatatg aatacttaag cccacgtata atgaggggat ggagcaagta aagggcgtta
     1681 atttaggcgg gtggttggtc ctagaacgat ggattagccc aagcgtgttc tcgggaacta
     1741 aggctcgcga tgaatattca ctctgctcag aattgggcgc taaggccgcc ggacggctta
     1801 aaatacatcg cgataacttc atcaccgagg atcatatcaa agaattgagc cgccaaggtc
     1861 tcaatacact gcgcctaccg gtcggctact ggttgtttgg ctccgagtcg ccgtttgtcg
     1921 atggggccga cgcttacgtc gacagagtct ttagctgggt ggataagtat gatttcaagg
     1981 ttattctcga ctttcatgcc gctccaggct cgcaaaacgg caacgaccat agcggccaag
     2041 ccggcgagat taactggtcg gagcccgaca atgtccttaa atcgttagaa ttcatcgaca
     2101 agctgacgcg gcgctacggt aaagaggagg gtcttgtcgg agtagaagtt ctcaatgagc
     2161 cagattgggg cattggctta cctaccttga tcgattatta cgctaaagcg gatgagatag
     2221 tcaagtctaa ctgtcatcct ggagtcagta ccatagttca tgacgccttc cgaccaaaag
     2281 agatggcgct agcactagat gctgccggac tcgatgtggt actcgatgtt cacctgtatc
     2341 aacttttcac gccggaggat agaacgctta acctcaaagg gcatatccga aaaactaagc
     2401 gcgactggaa gaagctgctg aaaaagctag gtcggtcacg tcagttactg gtaggggagt
     2461 ggagtgtagc catgcatgaa ctgtatcgaa aaatagacca gccggattgt cactatacac
     2521 caggcgacta tcgaaaatat tttctcgccc aatgccaggt gtttgaaaaa accaaagctg
     2581 gctggactta ctggacggcc cgcacggaag acggaggtat ttggagttat atcgatcagg
     2641 actttgaagt ctagtcaggc ac
//
LOCUS       DASSBW010000038        11004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1421356, whole genome shotgun sequence.
ACCESSION   DASSBW010000038 DASSBW010000000
VERSION     DASSBW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421356"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFG56_01045"
     CDS             complement(<1..447)
                     /locus_tag="VFG56_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622500.1"
                     /translation="MEKQATNTPVTDDQELANVLAGMNGQAAASATDDNKPADDPAAA
                     DLQYEETGDKAQVSDQTPAEEPVTEEVAAVPTLDELEKTVAADAAETPELDSPAPTPD
                     GQATTTSLSPELDSLKKDAVAELRPLVDKLDLPADEKFDTLLLIIRS"
     gene            complement(522..1730)
                     /locus_tag="VFG56_01050"
     CDS             complement(522..1730)
                     /locus_tag="VFG56_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6622501.1"
                     /translation="MLNNIRLAFTSLKRNRVRTALTTLGIVIGIASITLVLALSEGAK
                     QAITYQVDDLDDNIILIKPGHQLEHSLAVYSPYNTAITTTLTEEDLHSVAELPNVKSV
                     APAMFLTGALAYKQTNIPGPILATNDQFVTVLELELVSGQFIDDDTGRDSVVLGHDLA
                     LKLLGTDQAGGQQVKLKGRLHTVIGVMRSIDQPINLTGINLDDTAYISLADGKSFNQG
                     VAQIQQLNVQLEDPAKLESSVAKINQTIKSNHGGQEDFAVLGADAIASAGNQTFRMIA
                     SLTSLVALITLVVGGIGIMNIMLVTVSERTREIGIRKALGATDRNVLSQLLIESLLMS
                     LSGGITGVVIALLLDLFIAAQIAIAPVFSPYVIGTGLGAAVLVGVIFGLYPALKASRQ
                     DPIRALRDYH"
     gene            complement(1723..2508)
                     /locus_tag="VFG56_01055"
     CDS             complement(1723..2508)
                     /locus_tag="VFG56_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6622502.1"
                     /translation="MAKSKKPPLVIEMNAVTKVYGQDDSSHVALDQLDLKIEKGEFVA
                     IMGPSGCGKTTLLNLLGLLDSASEGKYKLNNRVVSKLSHAKRSHIRAHQIGFVFQSFN
                     LLPRLNILENVALPLSYQRTWRHKNLAAASKILKTFELETREYYMPDQLSGGQLQRVA
                     IARALVNNPSIILADEPTGNLDSKSSELIMNELRDIHLKGNTIVMVTHNPDLTTYASR
                     VIIMGDGKIMFDSKHPEAEVEKSTKQPLDSHLREIKEKLLTHA"
     gene            2751..2842
                     /locus_tag="VFG56_01060"
     tRNA            2751..2842
                     /locus_tag="VFG56_01060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2785..2787,aa:Ser,seq:gga)
     gene            2894..4483
                     /locus_tag="VFG56_01065"
     CDS             2894..4483
                     /locus_tag="VFG56_01065"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6622503.1"
                     /translation="MRKTPPKYIFVTGGVLSGVGKGITAASIGATLKARGVHVSMQKC
                     DPYLNVDAGTLNPAEHGECFVTKDGAETDLDLGHYERFLDVELTQNSATLSGRLLKQL
                     IEDERAGKFLGRTIQLVPHLTAAIKNAIRTSSVGSQIHIVELGGTIGDYEGLSFVEAI
                     REFASEVGRDNCVFVHVVYVPYLMASKEFKTKPAQNAIRDLRGFGISPDVIVARTDYE
                     TDDNIAGKLSLYGDVPEDAVALLPNADTVYRVPLTLEESGVAGLIAKRLGVTKPADMK
                     PWRALVKAATDIKKRSIKIAVIAKYLDNEDTYFSVIEALKAAAWAERVNLQFDWLDAE
                     KIDKSGCEVLEDYDGVLVPGGFGTRGVEGMIKAADCALEHGIPYLGLCLGLQVAVIAA
                     ARRGGLNTAASSEQEPNSKTPVIYIMDDQKGLESTGGTMRLGDYPAELVADSLVRKLY
                     GQGEIVERHRHRYEVNREFEDYFEKGGLTVSGQSPSGRLVEFVEAKNHPFFIATQAHP
                     EFRSRPMRPHPLFSGFIRSLK"
     gene            4619..6274
                     /gene="argS"
                     /locus_tag="VFG56_01070"
     CDS             4619..6274
                     /gene="argS"
                     /locus_tag="VFG56_01070"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET6622504.1"
                     /translation="MQSAIKEALEEAVKAQFDVETTVELNRPDTQFGDLATNAALVVA
                     KQAGQKPRDVAHKLADALEQVDGVGEVSVAGPGFINIFLTDEALVESITKTPNLSGSP
                     SRGLVYVVETNNPNPFKDLHIGHAYNCIVADTIANLIDQSGAQVHRVGYHGDVGLHVG
                     KSMWAILKEIDHDPARLDSIKPAERPKFMSDCYQKGALAYEEDGSAKAEIEELSKESF
                     NPEGDFKTVYDICKAWSFDYIDATLERLGSKPAEKHYLESAANDMGEKTVRAHIGDVF
                     SESSGAVVFAGEAYGLHTRVFIASRGTTLYEARDLGLMQLKQTDFHPAKSLIVTGNEQ
                     KEYFKVVFKAAELALPELAGVTENISTGTVKLSTGKMSSRTGQVLNIEWLFDELRNAL
                     VERGGSVDDDATLVGALRYQMLKTSIGNDLVFDIEKALSLDGNSGPYLQYAHARAASI
                     LRKTDQDVIRPSDESFDASERQLVVKLSQFEEALAKACADLTPHTICNYLYELAKEFN
                     RFYEVSRIVGDEREPLRLWLVERYKNTLSAGLKTLGISAPDKL"
     gene            6331..6837
                     /locus_tag="VFG56_01075"
     CDS             6331..6837
                     /locus_tag="VFG56_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HET6622505.1"
                     /translation="MAAQSSKNQTKAEAQAEARAAKKEARAAKAQAAREKAKAAKLKA
                     AESRTGKAVSGFTDFIRTQGVVGLAIGLAIGTQANTTVKSIVEGFINPVVAFILGSDK
                     GLTERKWYIVGQDNDTANYLVTLGDRQLVFAWGQVLSSLITLLAVAAVIYFVVKGLKF
                     DKLDKAKQ"
     gene            complement(6861..7163)
                     /locus_tag="VFG56_01080"
     CDS             complement(6861..7163)
                     /locus_tag="VFG56_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6622506.1"
                     /translation="MSTPKLEKRSGCIAGAMNIIGQKWTALILRDLSYGSQRFADLER
                     SIPEINPRTLSRRLDELEVEDVICRCQDSMAYELTKKGRDLTPVLESMASWGDKYC"
     gene            7249..7821
                     /locus_tag="VFG56_01085"
     CDS             7249..7821
                     /locus_tag="VFG56_01085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET6622507.1"
                     /translation="MKVQVIVGSTRPGRVSDRIAKWVVTRANQLEGVEAELVDLVDYE
                     LPMLDESMSPQYNPERKPNDIAKKWLGKLDEADGYILVTPEYNRSYSAALKNALDYID
                     FQFRRKPVGLVAHGSTGGAQAVAHLRGVIPGLQSFTVPNATFLVGRADLMFDEDGNLD
                     KSVQGPRVSLDNTLDELKWYAETLASARVS"
     gene            complement(7798..8439)
                     /locus_tag="VFG56_01090"
     CDS             complement(7798..8439)
                     /locus_tag="VFG56_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622508.1"
                     /translation="MRAQAYDNPNYHPDRTYNGPGEAIANLYLSENTEHARLWLPQNQ
                     LEWQSIDLLRPDIARQTMAERDIKLDWRSKHRMAHSAFILCDLDTETTERFDFTSLRR
                     NQTVIVGRRPNLRYSALSSDYVAQQHMMIRAGDDGWLNLNTTLAGDDSPIGLEVVDSA
                     RQAAIKFPSGTERYDYAKPIEPDPDLEKVRLRHVVALAGAAQLGLFLRNSGRR"
     gene            8526..9080
                     /gene="orn"
                     /locus_tag="VFG56_01095"
     CDS             8526..9080
                     /gene="orn"
                     /locus_tag="VFG56_01095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET6622509.1"
                     /translation="MVEVLNKHATEHLLWLDLEMTGLDPTRDRILEVAAIVTDWDFNE
                     VARFESGVGQDNAEITQLLDANPFYVKMKDNKRVLLEQSAASPAETAVESMLVDFIRS
                     NCDTHRPVILAGNSIHMDRQFINQYWPRVNQLLHYRMLDVSAWKLVFEAKFGTKVLKK
                     ETHRALGDIEESIAELRKYIDRIN"
     gene            complement(9184..9795)
                     /locus_tag="VFG56_01100"
     CDS             complement(9184..9795)
                     /locus_tag="VFG56_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622510.1"
                     /translation="MSKSWRRTTKHRQASDKELSKIFFLHYELEEIFYEEEIIDFMAA
                     ARQAVAGEFYFACEQNPRWKPNSDKKRFRGTDNYIIGVSDRPSELAQHITKQTHRQLI
                     CTKQLSGELRELSPKLVERIVARARYDIVLRAINAMIRAGEIRYDHGTGIWEVNQSNI
                     DDFVADRHYRKEVKQQMFNDRAWHGRPRRDRGRVPEGVSVSFA"
     gene            9919..10248
                     /locus_tag="VFG56_01105"
     CDS             9919..10248
                     /locus_tag="VFG56_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET6622511.1"
                     /translation="MLEHKQIEQWITALGEVILDYPFGEGVAVYKVDSTKGKMFALIE
                     EGSRPVRLSLKCDPKLAELLRDKYETVLPGYHLNKKHWNTIICSGQLPETELKDLIRH
                     SYELAKA"
     gene            complement(10238..10801)
                     /locus_tag="VFG56_01110"
     CDS             complement(10238..10801)
                     /locus_tag="VFG56_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622512.1"
                     /translation="MNPNDNFISPGSSLPGQSSDKKFFLIISLVVAVVLIIGGALLLL
                     GGGPSTKDQLNQLSIQLADLKNLTTESRDNLRAEATINLNSELQLTLTSDQMALSSEL
                     GIGAPDEEAMQAATDSQAASQLATAKTDGTYDKTYKKIVTQKLSAIAAQAADVYKSSS
                     NDQVKNSMQSVYDHFTALSQRLSEARL"
BASE COUNT         2669 a         2568 c         2958 g         2809 t
ORIGIN      
        1 agaacgaatg atcagcagta gggtgtcgaa tttttcgtca gccggtagat cgagtttatc
       61 gaccaagggc cgtagctcgg caacagcatc ttttttcagt gaatcaagtt caggcgacag
      121 actggtagta gtggcctgac cgtctggtgt cggagctggg gagtcgagtt cgggtgtttc
      181 ggccgcgtca gcagctacgg ttttttctag ctcatccaag gtcggtacgg cagctacctc
      241 ctcagttacc ggctcctcgg ctggtgtctg atcgctgacc tgtgccttat cgccagtttc
      301 ttcatattgc aaatcggctg cggctggatc gtcagccggt ttattgtcgt cggtagctga
      361 agcagccgct tggccattca taccagctag aacattggct agctcttggt cgtcagtaac
      421 cggagtatta gttgcttgtt tttccattcc cacctctgtc ttggttgtta ataacgctta
      481 aacttactat agcatatgcg ttttgggatt gtgaagggta tctagtggta gtcgcgcaag
      541 gctcggatgg ggtcttgacg tgaagctttg agagctgggt agaggccgaa gattactcct
      601 accaagacgg cagcacccaa accggtaccg atgacataag gactaaagac aggggcgata
      661 gctatctggg cggcgataaa caaatcaagc agcaaggcta taaccactcc agtgatgccg
      721 ccactcagac tcatcaatag cgattcgata agtagctgac taagcacgtt gcggtcggtc
      781 gcgcccaagg ctttgcggat accgatttca cgcgtcctct ccgacacggt caccagcatg
      841 atgttcataa ttccaatgcc gcctacgacc agcgtgatca aagcgaccaa gctggttagg
      901 ctggcgatca tccgaaaggt ttgattgccg gctgaagcga tagcatcagc gccaagcacc
      961 gcaaaatcct cctgaccgcc gtggttagac ttgatggtct ggtttatctt agccacgctg
     1021 gactcaagtt tagctggatc ctcgagctgg acattgagtt gttgaatctg agctacaccc
     1081 tgattgaaac tcttgccgtc agccagactg atataagccg tatcatcgag attgattccg
     1141 gtcaaattga ttggctggtc aatcgacctc atgacaccta taaccgtgtg gaggcggcct
     1201 tttagcttga cttgctggcc accagcttgg tcggtaccca gaagtttaag ggctaggtcg
     1261 tggccaagga ctacgctgtc acgaccggtg tcatcatcga tgaattgacc actgactagc
     1321 tcaagctcca aaactgtgac gaactggtcg ttggtggcca atattggacc gggtatattg
     1381 gtctgtttgt aggcgagtgc accggtcagg aacatagccg gtgcgacgga tttgacattg
     1441 ggcagttcgg cgactgaatg caaatcttcc tcggtcaatg tggtggtgat ggctgtgttg
     1501 taggggctat agacagctag actgtgctcc agttgatggc caggtttaat caggatgatg
     1561 ttgtcatcaa gatcatcgac ttgataggtg atagcttgtt tggcgccctc cgacaaggcc
     1621 agtaccaaag ttattgaagc gattcctatg acgatgccaa gcgttgtcag agcggtacga
     1681 acccggttac gtttgagtga cgtaaaggcc aaccggatat tgttaagcat gggttagtag
     1741 cttttccttg atttcacgta agtggctatc aagcggctgc ttggttgatt tttccacttc
     1801 ggcttcagga tgtttgctgt cgaacataat cttgccgtcg cccatgatga taacgcgcga
     1861 agcgtaggtt gtaaggtcag ggttgtgggt caccatgacg atagtgttgc cttttaggtg
     1921 aatgtcacgc agctcattca tgatgagttc cgacgactta gagtctaggt tgccggttgg
     1981 ttcgtcggcc aagattatac tagggttgtt gaccagagcc ctggcgatgg ccaccctttg
     2041 taattggccg ccggatagct gatccggcat gtagtactct cgggtttcaa gctcaaaagt
     2101 ctttagtatc ttggaggcgg cagctaagtt cttgtggcgc caagttcgtt gataactaag
     2161 tggcaaggcg acattttcca agatgttgag tcgaggcaac aaattgaagc tctggaaaac
     2221 gaagcctatc tgatgcgccc ggatgtgtga ccgtttagcg tgggataact tggagaccac
     2281 acgattattt aacttgtatt tgccttctga agcgctgtcg agcagaccaa gaaggtttag
     2341 tagagttgtt ttgccgcagc cggacgggcc catgatggcg acgaattcgc ctttctcgat
     2401 tttgagatct agctggtcaa gggcgacatg actagagtca tcttggccgt ataccttggt
     2461 tacggcgttc atttctatga ctagtggtgg tttctttgac ttggccatag tgacttcctt
     2521 tagtacgagt atagataatc gcccctgttg ttacaacata attagcggca attacaacgt
     2581 tattgttcaa ttagccatgg agattgatat aattggggct gttctacagc ttccgaaacc
     2641 aaggaaccgg tttaatcggc gactgtttag gaaaggtgaa aggaagaaca taggcttaag
     2701 tcgaagcttt tgctgtaagc aaaacgagac agagccgtag gttctgacgc ggagaggtgt
     2761 ccgagtggct gaaggagcac gcttggaaag cgtgtgtgcg gggcaactcg taccgagggt
     2821 tcgaatccct ctctctccgc caaatagaaa tccggtctta tgaccggttt tctatttgct
     2881 ataatttatc tacatgagga agacgccccc gaagtacata tttgttaccg ggggcgttct
     2941 ctctggtgtc ggtaagggca taacagcggc cagtatcggt gccactctga aagcgcgtgg
     3001 cgtgcatgta tcgatgcaaa agtgtgaccc ctatttgaac gttgatgccg gcaccttgaa
     3061 cccggccgaa cacggtgagt gcttcgtgac taaagatggt gccgagaccg acctggacct
     3121 tggtcactac gagcgctttt tagatgttga gctgacacaa aatagcgcca ctttgtccgg
     3181 gcgactcttg aagcaactga tcgaagacga gcgagccggt aagtttttgg gtcggaccat
     3241 ccaactagta ccgcacttga ccgccgccat caaaaacgcc atccgtactt caagcgttgg
     3301 atcccagatc cacattgtcg agctgggtgg caccatcggt gactacgaag gcttgagttt
     3361 cgtggaagct atcagggagt tcgcttcgga agtcggtcgt gataattgcg ttttcgtcca
     3421 cgtcgtttat gtgccatatc ttatggccag taaagaattc aagactaagc cggctcagaa
     3481 tgctatccgt gatctacgtg gttttggtat cagcccggat gttatcgtag cgaggactga
     3541 ttatgagact gatgacaata tcgcaggtaa actcagtcta tatggcgacg tgccggaaga
     3601 cgctgtggca ttgctgccca acgctgatac ggtttatcga gtgccactga ctttagaaga
     3661 atcaggcgtg gccggtctta tcgccaagcg tctaggtgtg accaagccgg ctgatatgaa
     3721 accatggcgt gccttggtca aagctgcgac tgatatcaaa aagcgcagca tcaaaattgc
     3781 cgttatcgct aagtacttag acaacgagga tacttatttt tcggtcatcg aagctttgaa
     3841 agctgctgct tgggctgagc gagtcaatct tcagtttgat tggttggacg ctgagaaaat
     3901 cgataagtcc gggtgcgaag tcttggaaga ttacgacggc gtgctagtgc cgggtggctt
     3961 cggtacacga ggtgtcgaag gtatgatcaa ggcggccgac tgcgctttgg agcacggcat
     4021 accataccta ggactgtgcc ttggtttaca ggtcgctgtt atagcggccg cccgccgtgg
     4081 gggattaaat acggccgcca gctccgaaca agagccgaat tctaagacac ccgtcatcta
     4141 catcatggat gatcaaaaag gcttggagtc taccggtggg accatgcgtc taggtgatta
     4201 cccggctgaa ctggtggctg actcgctggt gcgtaaacta tacggccaag gcgagatcgt
     4261 cgagcgtcac cgtcaccgct atgaggttaa tcgcgaattc gaggattact ttgagaaggg
     4321 cggcctcacc gttagcggcc agagtccaag tggccgcctg gttgaatttg tcgaagccaa
     4381 aaaccacccg ttcttcatcg ccactcaggc tcatccggag tttcgctcac ggccgatgcg
     4441 accgcacccg ttattctccg gctttattcg ctcgcttaag tgaaatgatg ctagctacta
     4501 ctgccaaagg tccagtaaaa taaagacccc cttttcctta aaatggggaa acattagcct
     4561 tcgtcagcag tagctaacat cgctcagact aaatgtccga taggttataa ttgagctaat
     4621 gcaatcagct atcaaagaag ctttagaaga agctgttaaa gctcagtttg acgtagagac
     4681 gactgttgag ttaaaccgac cggacactca gtttggtgat ttggcgacca atgccgcttt
     4741 agttgttgcc aaacaggccg gacagaaacc acgagatgtg gcccacaaat tagctgatgc
     4801 gctcgaacag gttgatggtg tcggtgaagt cagtgtggcc ggaccgggtt ttatcaatat
     4861 cttcttgacc gatgaggcct tagtcgagtc gatcactaag acgcctaact taagcggtag
     4921 cccatcgcgt ggtttagtct atgtcgtcga gaccaacaat cctaatccgt tcaaggatct
     4981 acatatcgga cacgcgtata actgcatagt ggctgacact atcgctaact tgatcgacca
     5041 gagcggtgct caagttcacc gagtcggtta tcacggtgat gtcggtttgc atgtcggtaa
     5101 aagcatgtgg gcgatcttaa aagagatcga tcatgacccg gcgaggctag atagcatcaa
     5161 accggccgag cggccgaaat ttatgagtga ttgttaccaa aaaggcgctt tggcctatga
     5221 ggaagatgga tcggctaagg ccgagattga ggagctgtcc aaggagagct tcaatccgga
     5281 aggtgacttc aagacagtct acgatatatg taaagcttgg agttttgact atatcgacgc
     5341 gacactggaa cggcttggtt ccaaaccggc tgagaagcat tacttggaat cggctgcgaa
     5401 cgacatgggc gagaaaacag ttcgagctca tatcggcgac gtcttctccg aatcgagtgg
     5461 cgctgtggtt tttgccggcg aagcatatgg gttacatacc agggtgttca tcgcttctcg
     5521 tggcacaacc ttgtatgagg ctcgtgacct aggtctgatg caactaaaac aaaccgactt
     5581 ccacccggcc aaaagcctga tagtaaccgg caatgagcaa aaagaatact tcaaagtcgt
     5641 cttcaaagcg gccgaacttg ctcttccgga actagccggg gtaacggaga acatctcaac
     5701 tggcacggtc aagctatcga ccggcaagat gagttcacgt accggtcaag tattaaatat
     5761 cgaatggttg tttgatgagc tcaggaatgc tctggtcgaa cggggcggct cggtcgacga
     5821 tgacgctacc ctcgtcggag ctcttcgcta ccagatgttg aagacttcga ttggtaacga
     5881 cttggtattt gacatcgaaa aagccctcag tcttgatggc aactccggcc cttatctaca
     5941 gtacgctcat gcacgggcgg cttcgatctt aaggaagacc gaccaagacg ttattcggcc
     6001 gagtgatgaa tcgtttgacg cctcggaacg tcagttggtg gttaaactta gtcaattcga
     6061 agaggctctt gcaaaagcct gcgccgatct aacgccccat accatctgta attacttgta
     6121 cgagttggca aaagaattca atcgttttta tgaagtttca cggatcgtcg gcgatgaacg
     6181 ggagcccctg aggttgtggc tggttgaacg ttacaaaaac actttgtcgg cgggcctgaa
     6241 gaccctcggc atctcggctc ccgataagct gtagcttgcg accaaccaac tttttagtta
     6301 atttataggg tagtaaaaga ggaataattg atggctgcac aatccagtaa gaaccaaacc
     6361 aaagccgagg cccaggctga ggccagagcg gctaagaaag aagctcgagc tgctaaggct
     6421 caagcagcca gagaaaaagc taaggcggcc aagctcaaag ctgccgaatc tcgtaccggc
     6481 aaggctgtct ccggcttcac cgatttcatc cgaacccagg gtgtggtcgg gttggctatc
     6541 gggttggcta tcggcactca agccaacaca accgtgaaat cgatcgtcga gggattcatc
     6601 aatccggtgg ttgcctttat ccttggttcg gacaaaggac tgacagaacg caagtggtac
     6661 attgtcggtc aggataacga cacggctaac tacttagtga cactaggtga tcgtcagttg
     6721 gtattcgcct ggggacaagt tttgtcgtcg ttgattactt tactggcggt agccgccgtc
     6781 atctatttcg tcgtcaaagg cttgaagttt gacaagctcg ataaagccaa gcaataagtt
     6841 atcttagttc ccgaagctgg ttagcagtat ttatcgcccc aggaggccat agattcgagg
     6901 acgggagtca ggtcgcgtcc ttttttggtc agctcataag ccatcgaatc ttggcagcgg
     6961 cagatgacat cttcaacttc cagttcatcc agtcgacgcg acagggtgcg cggattgatt
     7021 tcggggatgg aacgctctaa gtcggcaaaa cgctgggaac cgtaactcaa atcgcgcagg
     7081 atcaaagccg tccacttctg accgatgata ttcatggccc cggcgataca gccgctgcgc
     7141 ttctctaact taggtgtgct cataaaatac tttactttgt atagtaaatt gctatactga
     7201 ttatataaat actatcaaaa gtatatcata aatcatcaag gaggatatat gaaagtacaa
     7261 gttattgttg ggtcaactcg tccgggccgt gttagcgatc ggatcgcgaa atgggtagta
     7321 acccgggcta atcaattgga gggtgtcgag gccgagttag tcgatcttgt agactatgaa
     7381 ttgccgatgc tcgatgaatc gatgagcccg cagtacaacc ctgagcgtaa gccaaatgat
     7441 attgccaaga agtggctggg taaactagat gaagccgatg ggtatatctt agtgactcca
     7501 gagtataacc ggtcttattc ggctgcttta aagaacgcgc tcgactatat cgattttcag
     7561 tttaggcgta aacctgtggg tctagttgca cacggctcca ccggtggtgc acaagctgta
     7621 gctcacttgc gcggtgtcat tcctggccta cagtctttta ccgtgccgaa cgctacattc
     7681 ctagttggcc gggccgactt gatgtttgac gaagacggca atctcgacaa atcggttcaa
     7741 ggaccaagag tttcgttgga caatacgctt gatgaattga agtggtacgc tgaaactcta
     7801 gcgtctgcca gagtttctta agaacaaccc caattgggcg gcaccggcca atgccacgac
     7861 gtgtcttaag cggacctttt caagatcagg atcgggttca ataggtttgg cgtagtcata
     7921 gcgctcggtg ccgctaggaa acttgattgc tgcttgtctg gctgagtcaa caacttccag
     7981 gccgatcggt gaatcgtcgc cagcgagagt agtatttaga ttcagccaac catcatcgcc
     8041 agctcttatc atcatatgtt gctgagcaac atagtctgag ctcagtgctg aatagcgcaa
     8101 attgggtcga cgaccgacga tgacagtttg gtttcgtctg agtgacgtga aatcgaaccg
     8161 ctcggttgtc tcggtatcaa gatcacacaa gatgaaggcg gagtgagcca tgcggtgctt
     8221 gctccgccag tctaatttga tgtcacgttc agccattgtt tgacgggcga tgtctggtcg
     8281 aagcaaatca atcgactgcc actctagctg gttttgcggc agccacagcc tggcgtgttc
     8341 ggtattttca gataagtaga gattggctat agcttcaccc gggccattgt aggtacggtc
     8401 aggatgatag ttgggattat cgtaagcttg agcgcgcata tttggttttt aaagcctctt
     8461 taagcttaac tataatccgc ttagctgcag aaataaagca gattttctct gctacaatct
     8521 tagggatggt tgaagtactc aacaaacatg ccaccgaaca ccttttgtgg ctggatctgg
     8581 aaatgactgg cctcgacccg acccgcgacc gtatcttgga ggtggctgcc atagtgaccg
     8641 actgggactt taacgaagtc gcccggtttg aatctggggt cggtcaagac aatgctgaaa
     8701 tcactcaact gcttgatgct aatccgtttt acgtcaagat gaaggataac aagcgagtgc
     8761 tgctcgaaca gtcagcggct agcccggccg aaacggcggt ggaatcgatg ctggttgatt
     8821 ttattcggag taactgtgat acccaccgac cggtcatcct agccggtaac tctatccaca
     8881 tggatcgtca gtttatcaat caatactggc cacgagtcaa ccaattgctg cattatcgca
     8941 tgctcgatgt atctgcttgg aagctagtat ttgaagctaa gtttggcact aaggtcttaa
     9001 agaaagagac tcaccgggct ttgggagaca ttgaggaatc gattgctgag cttcgtaagt
     9061 atatagatcg catcaattaa aatacccctc cttctacttg agaaagaggg gtgtcgcgcc
     9121 gtggttccgt agagtcaccc tgttatggct ttctcatcgt actacacaga cgcggccagc
     9181 ttctcatgcg aagctgacag acacaccttc cggtactcga ccgcgatctc gcctaggccg
     9241 accgtgccaa gcgcgatcgt tgaacatctg ctgcttgacc tccttgcggt agtggcggtc
     9301 ggccacgaag tcgtcgatgt tgctctggtt tacctcccag ataccagtgc cgtggtcgta
     9361 gcggatttcg ccggccctga tcatggcgtt gatggcccgt agaacgatat cgtagcgagc
     9421 tcgtgccacg attcgctcca ccagcttggg cgacaactcc cgaagctcgc ccgatagctg
     9481 cttagtgcag atcagctgcc gatgcgtttg cttggtgatg tgttgcgcca gctcgctcgg
     9541 ccgatcgctc accccgatga tgtagttgtc ggtgccgcgg aagcgcttct tgtctgaatt
     9601 cggcttccac cgcgggttct gttcgcaagc gaagtagaac tctccggcaa ccgcctgacg
     9661 ggcagcagcc atgaagtcga tgatctcttc ctcgtagaag atctcctcga gttcgtagtg
     9721 caagaagaaa atcttggaca actccttgtc actggcctgg cggtgtttcg tggtcctgcg
     9781 ccaactcttg gacacagttc ctccccgatg tgtgtttggt gctaagcagt ctctctgctt
     9841 agttttattt ataaaccatt agcggtatgg tggcaaataa ttactttgat tgtccggcta
     9901 tttggtaaca taaggttgat gttagaacac aagcaaatcg agcaatggat aaccgcctta
     9961 ggcgaagtaa tcttggatta tccgttcggc gagggcgtag cagtttataa ggtcgatagc
    10021 accaagggca agatgttcgc ccttatcgaa gagggtagca ggccggtgcg cttgagtctc
    10081 aagtgcgatc ccaagctagc tgagctgctc cgcgataaat acgagacagt gttgccgggt
    10141 tatcacctga ataagaaaca ctggaatact atcatctgct cggggcaatt acctgagact
    10201 gaacttaagg acctaatacg ccatagctac gagctggcta aagcctagct tcgctcaacc
    10261 gctgactaag tgcggtgaag tggtcataaa ccgattgcat agagtttttc acttggtcgt
    10321 tgctggatga tttataaaca tcggcggcct gagcggctat agctgatagt ttctgtgtaa
    10381 cgatcttctt gtaagtctta tcgtaggtgc catcggtttt ggcggtagct agctggctag
    10441 ctgcttggct gtcggtggcg gcctgcatag cctcctcatc aggtgcaccg atacccagtt
    10501 cacttgaaag agccatctga tcgctggtta gagtcaactg cagctcacta ttcaaattga
    10561 ttgtggcttc agcccgtaaa ttgtctcgag attcggtagt taagttttta agatcggcta
    10621 gttggatcga tagttggttg agttgatctt tggtcgacgg accgccgccg agcagcagta
    10681 gggcgccacc gataatcaat actacagcta cgactagact gatgattaga aaaaactttt
    10741 tgtcagaaga ttgcccgggt aatgaggagc cgggcgagat gaagttatcg ttaggattca
    10801 tgagattagt ttaccattag cgtttacaga ggtatagggt gctacaataa ttgaaagtgg
    10861 agaaaccaat tggacgctaa agaagaagtt cgcgaacggc tcaatatcga agatgtcatc
    10921 ggccagtatg tgcagttgaa gcgggccggc cgtaactgga aaggcttaag cccattctcg
    10981 agcgaaaaaa ccccgagttt tatg
//
LOCUS       DASSBW010000039         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_991604, whole genome shotgun sequence.
ACCESSION   DASSBW010000039 DASSBW010000000
VERSION     DASSBW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991604"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /gene="trmB"
                     /locus_tag="VFG56_01115"
     CDS             <1..528
                     /gene="trmB"
                     /locus_tag="VFG56_01115"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET6622513.1"
                     /translation="IDVVEVGAGTGLFAVELASRHPELNFAAIDVKADRLQKGAYVAM
                     EQGLHNVFFIRARADQLDELFDPASLRQVWLTFPDPHPKKRSAGRRLTHPTFLKKYAD
                     LLNSEGALYLKHDNRDFFDWSMEQLVAEKWRLDELSFDLHESELSDEYKATTTYEARW
                     LSEGLRTNFLKAKIS"
     gene            complement(515..1648)
                     /locus_tag="VFG56_01120"
     CDS             complement(515..1648)
                     /locus_tag="VFG56_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622514.1"
                     /translation="MIETLEPNYEFEPQPNREIEKKFTPLFPEKLDLFRESAQPMEQF
                     YLSHPDEEFCLRLREYSKDGWLVHTATLKSRGEITAQGLDRVEIETPIDPATYDLYRQ
                     DDLPTIRKLRAEPVKNIAIDFFEDGGVLIESENPISLTAFIGRHALDNGLAEVTGDRT
                     ADNEWRAHFKYRQEHGGQSVSESTIDLDVGSIVDQIQQQTTKSKMTTVAICGRSGSGK
                     STLVQEITRQLNLNDTESVVLSTDDYHRGKTWLEHHKGGVWTEWDAPIVYDTAELAED
                     LHRLRAGQAIVKRRFDFESEEPIFDGVISPASVVIIEGIYAGSDDIKPTFRHDLPTPL
                     ATCIGRRLLRDLKERPQFGDPVKSLRYMIEQAEPAYRRQLKKS"
     gene            1742..2413
                     /locus_tag="VFG56_01125"
     CDS             1742..2413
                     /locus_tag="VFG56_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6622515.1"
                     /translation="MLDNTDVSHHIQKYILSVLMHQKSARFRDLRPPRTDTNLFAYHL
                     KLLAKYGLIEKAEMGYRLAPKGSAYVDRVNADKLFIRNQPKIITMLVIQNSNGDVLLL
                     RRDKQPYIDTWTLPYGKLHLDDASIKAAAEREVYEKLGLKGQDLSHAGDCYIRVKSDG
                     QILSSTLAHVFSFNNDDIDISERLQWFRPHKLDNLDLAPAVQEIMSRTFFRDPYFFEE
                     FEEAI"
     gene            complement(2406..>3044)
                     /locus_tag="VFG56_01130"
     CDS             complement(2406..>3044)
                     /locus_tag="VFG56_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET6622516.1"
                     /translation="ELMDLSHIKLAGDHVRHNAYLVERAIERLGGETAFVSEDSAFSD
                     SSTLVSPGPEEANAAPALAIIDAISSFPGTARRMEKLADNLYSDYGHHPVEIAATLQL
                     ARELSDHVVLVYQPHQNVRQHEVKDDYTACMELAEEVYWLPTYLSREDPDLEVLTPEQ
                     LSQNLTNRDKVHLADLNDELWSAIKKARDDGKLVLCMGAGSIDGWLRQQLAR"
BASE COUNT          748 a          699 c          751 g          846 t
ORIGIN      
        1 attgatgttg tcgaagttgg agctggcacc ggcctgttcg ctgtggagct agcttctcga
       61 caccctgaac taaacttcgc ggccatagac gtcaaagccg acaggttgca aaaaggcgct
      121 tatgtggcga tggagcaggg cctccataac gtcttcttta ttcgagctcg ggctgatcaa
      181 ctggatgagc tatttgatcc cgcaagctta aggcaggtct ggctgacttt tcctgatcct
      241 cacccaaaga agcgatcggc tggtcgacga ctgacacatc cgacatttct taaaaagtac
      301 gcggatctac ttaactcgga aggtgcgctt tacttaaagc atgataatcg tgactttttt
      361 gactggagta tggagcaatt ggtggcagag aagtggcgac tggatgaatt aagttttgat
      421 ttgcacgaat ccgagcttag tgatgaatat aaagccacta cgacctatga agctcgttgg
      481 ctgagtgaag gtctcaggac taactttcta aaagctaaga tttcttaagt tgtcttcggt
      541 aggctggttc ggcttgttcg atcatgtagc gcagactttt gaccgggtcg ccaaactgtg
      601 gtctctcttt tagatcacgt aacaatcggc ggccgataca ggtggctagt ggtgttggca
      661 gatcgtgacg aaaggtaggc tttatatcgt cgctgccggc gtatatgcct tctatgataa
      721 ctactgaggc cggcgagatt acaccatcaa aaataggttc ctcactctcg aaatcaaatc
      781 gccgcttaac gattgcctga ccggcgcgaa gtcggtgcaa gtcttcggct agctcggccg
      841 tatcatagac gatgggcgca tcccactctg tccacactcc gcctttatgg tgctccagcc
      901 aggttttgcc gcggtggtaa tcgtcagtcg ataagaccac agactcggta tcgttgaggt
      961 ttagctgcct agtaatttcc tgaacaaggg ttgatttgcc ggaacccgac cggccacaga
     1021 ttgcaaccgt cgtcattttt gacttggtgg tctgttgttg aatttgatct actatcgaac
     1081 cgacatctag atcaatggtc gactccgaga ctgattggcc accgtgctcc tgacggtatt
     1141 tgaagtgagc ccgccactca ttgtcggcgg ttctgtcgcc tgtaacttca gctaagccat
     1201 tgtctagagc atgacgaccg ataaaagccg tcagggaaat tggattctca ctctcgatta
     1261 gaaccccgcc gtcctcaaag aagtcgatag caatgttttt tacgggctcc gctctaagtt
     1321 tgcgaatggt tggtagatcg tcttgcctgt aaagatcata agtggcaggg tcgatcggcg
     1381 tttcgatctc aactcggtcc aatccttgag ccgttatttc accacggctt tttaaggtgg
     1441 cggtgtgaac tagccaaccg tctttgctgt attctcttag tcgcaaacag aattcttcat
     1501 cagggtggct caaataaaat tgttccatcg gctgggctga ctcgcgaaag aggtctaatt
     1561 tttcgggaaa tagaggggta aacttttttt ctatctcacg gttgggctgt ggctcaaatt
     1621 cataattagg ctccaatgtt tcaatcatac ataactacct ttctgtcact atttaacaga
     1681 tgactttttg ataataatcg actatattgg taatatctat aaagtttaat ttgactcaaa
     1741 tatgcttgat aatactgatg taagccacca catccaaaaa tacattttga gcgttttgat
     1801 gcatcaaaaa tccgctcgtt ttcgtgacct gagaccaccg cggactgata ccaatttgtt
     1861 tgcctatcat ctgaagctgc tcgccaaata cggcttgatc gagaaagctg agatgggtta
     1921 tcgtttggcg ccgaaaggat cggcctatgt tgatcgggtc aacgccgaca aattattcat
     1981 acgtaaccag cccaaaatca tcactatgtt agtgattcaa aattctaacg gcgacgtact
     2041 gcttctacgg cgagataaac agccttatat cgacacttgg acattacctt atggcaagct
     2101 ccatttggat gacgcgtcaa tcaaagctgc cgccgagcgg gaggtttatg aaaagctagg
     2161 actcaaaggt caagatctaa gccatgcagg ggattgttat atccgtgtta aaagtgacgg
     2221 tcaaatcctg tcgtcgactc tggctcatgt atttagtttt aataacgacg atatcgatat
     2281 ttctgagagg cttcagtggt ttaggccaca caaattggac aatctcgatc tggcgccggc
     2341 agttcaagaa attatgagcc ggacattttt ccgtgatccg tacttttttg aagaatttga
     2401 agaggctatc tagctaattg ctgacgtaac caaccatcga tagaaccggc acccatgcac
     2461 aggactaact tgccatcatc acgtgctttt ttgatagcgc tccaaagttc atcgttgaga
     2521 tcggctaagt ggactttatc tcgattggtc agattttgac ttagttgctc gggcgtcagt
     2581 acttccaggt ctggatcttc acgcgataga taagttggca gccagtaaac ttcttcggct
     2641 aactccatgc aggcggtata gtcatctttg acttcgtgtt gacggacgtt ttgatggggt
     2701 tggtaaacca agacaacatg gtcggacagt tcgcgtgcca gctgcaaggt ggcggcgatt
     2761 tcaactggat ggtggccgta gtcgctgtac aagttatcgg ctagcttttc catgcgccgg
     2821 gcggtacccg gaaaagagct gatggcgtcg atgatagcta gagccggagc ggcgtttgct
     2881 tcttccggac cgggtgacac taaagtagag ctgtctgaaa aggcagagtc ctcagaaaca
     2941 aaagccgttt cgccccccag tctttcgata gcccgctcga ccaggtaagc attgtgcctg
     3001 acgtggtcgc cggctaattt gatgtgcgat agatccatca attc
//
LOCUS       DASSBW010000040         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_587569, whole genome shotgun sequence.
ACCESSION   DASSBW010000040 DASSBW010000000
VERSION     DASSBW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587569"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..119
                     /locus_tag="VFG56_01135"
     CDS             18..119
                     /locus_tag="VFG56_01135"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622517.1"
                     /translation="MKDMGRVIGAVKQKAGNSADGALVARLVKEQLQ"
     gene            124..675
                     /locus_tag="VFG56_01140"
     CDS             124..675
                     /locus_tag="VFG56_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET6622518.1"
                     /translation="MIIFFGPAGAGKSVQGQILSARHGWRHLGAGQLLRDTHDHELIK
                     EMQTGALVDETRVNQIMAGALKAARDLDHVILDGYPRQLTQAQWLVDNQPEHERSIKM
                     VVVLEVPKSEILRRLKVRGRADDTEAAIEERLRIYRQEIYPILSYLTDEKVYVVHIDG
                     AGSVGQVHDRIEAELEAQGLVQK"
     gene            672..1430
                     /gene="map"
                     /locus_tag="VFG56_01145"
     CDS             672..1430
                     /gene="map"
                     /locus_tag="VFG56_01145"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6622519.1"
                     /translation="MITGQKSQSQIESMRQCGRILAEIFHELKNQVQAGVRERQLDAW
                     VEREIKARGAEATYKTPEVNFPGAICISTNDQIVHSVPSDYVLEQGDVVSFDMVISYQ
                     GMKTDSAFTMVVDEAPSGDKKRLIEFTEKSLYAGIDAINGPVKVGDISSAVGTVLDGA
                     GLGVIRELVGHGIGHLMHCPPDIPNYGPSGMGPLLVPGETIAIEPMATLGSAAINPHH
                     PDGWTITTKDGSLAAHFEHTVLITESGAEILTEL"
     gene            complement(1512..1862)
                     /locus_tag="VFG56_01150"
     CDS             complement(1512..1862)
                     /locus_tag="VFG56_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622520.1"
                     /translation="METLKLSDKELIDELVRFIGPTLTAFTAHSTVRAEFVLDEWRNG
                     TGVLHPGKRERLEVAYKLLNDVAEADGEDTARAWFIGANSGSNRISPAESIRRGEFTD
                     AKASARSLIRDEWL"
     gene            2107..3357
                     /gene="ftsA"
                     /locus_tag="VFG56_01155"
     CDS             2107..3357
                     /gene="ftsA"
                     /locus_tag="VFG56_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET6622521.1"
                     /translation="MQEASRYAVGLDIGTTTVRCVVGHVDGPGQAPTIIGVAAEAGKG
                     LRKGNVVTLNDVAQAADKALETAERLSGHRIEAATVSINGTHIVGLKSTGVIAVGAVD
                     HEINLSDLARVEEAATVVQLPANREILDVTPINYKLDNQSGIKDPLGMTGVRLEVDAY
                     MITALGPHLKNLEKAITLSDTQPRQIVVAGLAAAKTALNDQQKENGVALVDIGGTTTN
                     VVVYEEGDLLHLGVVPYGSVNITNDLAIGLKTDLAVAERVKLEHSVASADFRKDSKKT
                     VTVKLDGHTHEFDTETIDEIVEARLEEIFEEVNKELKKAGRQAKLPGGVVLSGGGAKL
                     TGVADYAKAVLKLNARVAEAPELSGVADKVTTPDFATALGLMMIDLDAAPAVARTNGS
                     ETNAGRLLSGSMKAAGNLFKKLRS"
     gene            3457..>3773
                     /locus_tag="VFG56_01160"
     CDS             3457..>3773
                     /locus_tag="VFG56_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET6622522.1"
                     /translation="MPEVKPSDIQTFASIKVVGVGGAGGSAVNRMKEAGLQNVEFIAI
                     NTDAQALHNSKADVKIHIGKETTRGLGAGADPMAGQAAAEESRDEIKQALNGADMVFV
                     TIGA"
BASE COUNT          977 a          901 c         1030 g          865 t
ORIGIN      
        1 agattcatcc ggaaggcatc aaggacatgg gccgagttat cggcgcagtc aagcaaaaag
       61 ctggtaattc ggccgatggc gccttggttg ctcgcttagt caaagaacag ttacaataaa
      121 cttatgatta tattctttgg tccggctggt gccggcaaat ccgtccaagg ccaaatttta
      181 agcgctcgcc atggctggcg ccatttgggt gctggccagc tacttcgcga cactcatgat
      241 catgaattga tcaaagaaat gcaaaccggt gcgctggtcg acgaaaccag agtcaaccag
      301 atcatggccg gggcactaaa ggccgccagg gacttagacc atgtcatctt ggatggttat
      361 ccgcggcaac tgacacaggc gcagtggtta gtcgataatc agccggaaca tgaacggtct
      421 atcaaaatgg tggtagtgct ggaagtaccg aagtcagaaa tcctaaggcg cctcaaagtg
      481 cgtggacgag ctgatgatac tgaggccgct atagaggagc gcctacggat atatcgccaa
      541 gagatctatc ctattctgag ctacttaact gatgaaaaag tctatgtagt ccatatagac
      601 ggcgccggta gtgttggcca ggtccacgat cgaatcgagg ccgaactgga ggctcaaggg
      661 ttagttcaaa aatgataacc ggtcagaaaa gtcagagtca gatagagtcg atgcgccagt
      721 gtggccgcat tttggcagag attttccacg aactaaaaaa ccaagtgcaa gccggcgtca
      781 gagaacgcca actcgatgcc tgggtggagc gcgagatcaa agctcgtggc gccgaagcta
      841 cctataagac gccagaggtt aacttcccgg gtgctatatg tatctcaacc aatgatcaaa
      901 tcgttcattc agtgccaagt gattatgtcc tggagcaggg cgatgtagtg agtttcgaca
      961 tggtgattag ttaccaaggt atgaaaaccg actcggcttt cactatggtg gttgacgaag
     1021 cgcctagtgg tgataaaaaa cgattgattg aatttaccga gaaaagttta tatgccggta
     1081 tcgatgctat caacggtcct gtcaaagtcg gcgatatcag ctcagctgtc ggtactgttt
     1141 tggatggagc cggccttggt gttatccgag agctagtcgg tcatggtatc ggtcatctga
     1201 tgcattgccc accggatatt ccgaactatg gtccgtcggg catgggaccg ctattggtac
     1261 cgggtgagac catagcgatc gagccgatgg cgacactcgg ttcggccgct atcaacccac
     1321 atcaccccga tggctggacg atcacgacca aagacggtag cttagctgcc cactttgagc
     1381 acacagttct gattaccgaa tccggtgcag aaatcttgac agagctctaa ctagccgcaa
     1441 cctgactagt cgaattattc aactgttcgg gttgcgggag acagaggaat ctgtaacccg
     1501 cggagaggtg gctataacca ctcatccctg atgaggctac gggccgaggc cttggcgtca
     1561 gtgaactcac cacgacggat ggactcggcc ggagagatcc ggttggagcc ggagttggca
     1621 ccgatgaacc aagcccgggc ggtgtcttca ccatcagctt cagcgacatc gttgagtagc
     1681 ttgtaggcca cctccagtcg ttctcgcttg ccggggtgga gtacgccggt gccgttgcgc
     1741 cattcatcca ggacgaactc tgcccggacg gtggaatgag ctgtgaacgc cgtcagtgtc
     1801 ggaccgatga accgaaccag ttcatcgatc agctccttat ccgatagctt gagtgtttcc
     1861 atgttggact tcctctgtct cgtaggtgcg ctccttggtt gttagaaaga gccatatgga
     1921 taatactatt ttgtttgatc atagctcaag cttaagcttc atcgtccaat atctaatgtc
     1981 cagagtccag agtccaacgt ccaacgtcta acgtccaatt aatatactca ctatggtcta
     2041 cataattatt cagcttagct taagcatgat ttgccacagc ccatcgctaa agttatactc
     2101 tagggtatgc aagaagcttc tcgttacgca gtcggtttag atatcggtac gactaccgtt
     2161 cgttgtgtgg ttggtcatgt cgacggacca gggcaagcac cgactattat cggtgtggct
     2221 gccgaagccg gcaagggact gaggaagggt aatgtcgtta ctttgaatga cgtggctcag
     2281 gcggccgaca aagctctgga gacagccgag cgtttaagcg gtcatcgcat agaagcggct
     2341 actgtcagca tcaatggtac ccatatagtt ggcttgaaat ccaccggggt tatagccgta
     2401 ggtgcggtcg atcatgaaat caacctgagt gacctggctc gggtagaaga ggctgccact
     2461 gtagttcaat taccggctaa tcgcgagatc ttagacgtca cgccgattaa ctataagctg
     2521 gacaaccaat caggtatcaa agatccgctt ggtatgaccg gcgttcgcct ggaagtcgat
     2581 gcttatatga ttactgcgct tggaccacat ctgaaaaact tagaaaaagc cattactcta
     2641 agcgacacgc agccgcgaca aatcgtagta gctggtctgg ccgccgccaa gacggcgctt
     2701 aacgaccagc aaaaagaaaa cggcgtagct ctagttgata taggtggcac tactaccaac
     2761 gtcgtcgttt acgaggaagg cgatctgctg catcttggtg tcgtgccata cggtagcgtt
     2821 aacatcacca atgacttagc tattgggttg aagaccgatt tagccgttgc cgaaagggtc
     2881 aagcttgagc actctgtcgc cagtgctgac tttcgcaagg attcaaaaaa gaccgttacg
     2941 gtcaaacttg acggccatac tcatgagttt gacactgaga ctatagatga gatcgtcgaa
     3001 gcccgtctgg aagagatctt tgaagaggtc aacaaggagc tcaaaaaagc cggtcgtcaa
     3061 gccaagcttc cgggaggcgt ggtcctgagc ggcggtggag ctaagctgac cggcgtggct
     3121 gattatgcca aagcagtttt gaagctcaat gcccgggtag ctgaagcgcc ggaactatcg
     3181 ggtgtagccg ataaggttac gacaccggat ttcgctacgg cactgggtct gatgatgata
     3241 gacctcgatg ctgcgccggc agtagcacgg acaaatggca gcgagaccaa cgccggcaga
     3301 ctactttctg gttcaatgaa ggctgctggt aatctgttta agaaattgcg ttcataaatt
     3361 ttgctcgcta aacttgatgc gatagacgct atgaacacat gtttggtacg atgaaaaaag
     3421 ccatgtataa ttacgctaga acaaaagggg aaataaatgc ctgaagtcaa accaagcgac
     3481 atacaaacat ttgccagtat caaagtagtc ggcgtcggtg gcgccggcgg ttcagctgta
     3541 aaccggatga aagaggccgg tctgcagaat gttgagttca ttgctatcaa caccgacgct
     3601 caggctctcc acaactccaa ggccgacgtc aaaattcata tcggcaaaga aactactcgg
     3661 ggacttggcg caggagccga cccgatggcc ggccaggctg ctgctgagga atctcgtgat
     3721 gagatcaagc aggcgctaaa tggtgccgac atggtcttcg ttactatcgg tgc
//
LOCUS       DASSBW010000041         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_928192, whole genome shotgun sequence.
ACCESSION   DASSBW010000041 DASSBW010000000
VERSION     DASSBW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928192"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFG56_01165"
     CDS             complement(<1..794)
                     /locus_tag="VFG56_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6622523.1"
                     /translation="MSVEQERVPELATVEATSTAVELYSPRHSRRKRRMAKSAIEGFT
                     LLEAVESPWAEVRTQLEDRIIGQPAAIDAIIEALEGGDVRMPSDKRPVANFAFLGPTG
                     VGKTETAKSLAEFIDLSRKFVNIDCSNFSHGHEVANLIGAPAGYVGYDQEPLLSKEKV
                     EGDGTVILFDEIEKGSESLYNLMLQIMENGELQMRNGDITSFRGAIIIMTSNLGAKDM
                     ADQLSPTRLGFTSLDQQPDTNHLEEVARKKFSEHFRAEFINRLTAMV"
     gene            960..1205
                     /locus_tag="VFG56_01170"
     CDS             960..1205
                     /locus_tag="VFG56_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622524.1"
                     /translation="MAFNHYAKLKRILAEQPAGWYIRRIDQPTSAKTFSGETVHYSHY
                     YRLYSADDQVIPYGKFQQIERLAQTLGIPTEALPVVD"
     gene            1306..1629
                     /locus_tag="VFG56_01175"
     CDS             1306..1629
                     /locus_tag="VFG56_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622525.1"
                     /translation="MSVNVGQLLPQELVQNGMPVNEAGDHDADGAIRLYRALPRDYRW
                     RKAIVSSDGLWLLDGDRPGSLHFNHASYGNGVSEFEAAEKILTGAGFDKEDYLLDSGH
                     YIYKH"
     gene            complement(1692..2072)
                     /locus_tag="VFG56_01180"
     CDS             complement(1692..2072)
                     /locus_tag="VFG56_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6622526.1"
                     /translation="MQTLHISTEHKRQVIDITDQVAATLPDDEGMLSVFVQHTTAALT
                     IMDLDPGTDDDFLDFLNSLVPNIDWRHPHDPAHAPDHLLASLVGPSAVVPYIKGRLQL
                     GSWQRLVLVEFDGPRELSVIISSK"
     gene            complement(2080..2577)
                     /locus_tag="VFG56_01185"
     CDS             complement(2080..2577)
                     /locus_tag="VFG56_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HET6622527.1"
                     /translation="MTTNELMADGFDRLKPVVHTALKDAAADELAYRPYDKGNSISWL
                     VWHLSRVQDDHIAELADREQVWLSGGWHDEFGLPFEKQATGYGQDSDQVSQVQVDGEL
                     LKGYFDAVQQATSDFLTGLKPEDYDRIIDESWDPPVTLGVRLVSVLVDDWQHAGQAAY
                     VRGLL"
     gene            complement(2605..>3222)
                     /locus_tag="VFG56_01190"
     CDS             complement(2605..>3222)
                     /locus_tag="VFG56_01190"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET6622528.1"
                     /translation="GWLVIYRNEGDTMNVMNYSAPRPSRLAKLSAWVLALVLVVAVAG
                     AAYYFLGQRSERADLERRNQQLQAQVQQLQNDKQSNQSDQLPADRSRPTNIFESSKGV
                     DITVYSPLAQDTVDSPLGVIGKAPGNWSFEASFPVVLKDANGQVIAETTAKMQGDWMT
                     DEDVPFAASLTFDKPATSTGTLVLQNDNPSGEPENDDSVSIPVKF"
BASE COUNT          731 a          914 c          812 g          765 t
ORIGIN      
        1 accatggcgg tcagtcggtt gataaactct gcccggaagt gctcactaaa tttcttacgg
       61 gcgacttctt cgaggtgatt ggtatcaggc tgctgatcta acgaagtgaa gccaagccgg
      121 gttggactga gctgatcggc catatccttg gcgccgaggt tgctggtcat aatgatgatg
      181 gcaccgcgga agctagtgat gtccccgttc ctcatctgta gctcaccgtt ctccatgatc
      241 tgtaacatca ggttatagag tgactctgag cctttttcta tctcgtcgaa caagatgacc
      301 gtaccgtcac cctcgacctt ttccttagaa agtagcggtt cttggtcgta gccgacataa
      361 ccggcgggag cgcctatgag gttggctacc tcgtgaccat gtgagaaatt agaacaatca
      421 atattgacga atttgcggct caagtcgata aactccgcca aggacttagc cgtctcggtc
      481 ttgcctactc cggtcgggcc caggaaagca aagttagcga ccgggcgttt gtcgctcggc
      541 atacggacgt cacccccttc cagagcctcg atgatagcat cgatggccgc cggctgaccg
      601 atgatacgat cctctagctg tgtccggact tcagcccagg gactttccac cgcctcgagc
      661 aaggtgaagc cttcgatggc cgacttggcc atgcgacgct tccggcggct gtgcctcgga
      721 ctgtataatt cgaccgcggt cgaagtcgcc tccactgttg ctaattccgg cactcgttcc
      781 tgctctacgc tcatagtttt ccctttcttt ttgtgtcaga aacgcaaaaa tcgccctatt
      841 taaaggcgat cggttgtctc acactcactt ccatatacaa gttatacccc cattgcgctg
      901 acctgtaaag cctgctcgac tctgtcttgc ctatagcttt gtttgtaaaa tagatctcaa
      961 tggcatttaa ccactacgcc aaactaaaac gcattctggc cgaacagccg gctggctggt
     1021 atatcaggcg tatcgatcag ccaaccagcg ccaagacctt tagcggcgag acagtacact
     1081 actcacacta ctaccgtctc tactcggccg acgaccaagt cataccatac ggcaaattcc
     1141 aacaaatcga acgattagcc caaactttgg gcataccgac agaagccctg cccgtcgtcg
     1201 attaaccctc aatcagtcct ctactatctt ttaaaaccta ctagtaaaaa ccgccgccag
     1261 attgtacgct ggagtcagtc ctgcagtcta cctacgggag gaactatgtc agtaaatgtg
     1321 ggtcagctcc ttccgcagga gctggtacaa aacggtatgc cggtaaatga agccggcgac
     1381 cacgacgccg atggcgccat ccggttgtac agggcgctgc cgcgggacta ccgctggcgt
     1441 aaggctatcg tgagcagcga tggcctatgg ctgctcgatg gtgaccggcc cggatcactc
     1501 cacttcaacc acgccagcta cggcaacggt gtgtcggaat ttgaagcagc agaaaaaatt
     1561 ctgacgggtg ccggtttcga taaggaggac tacctcctag acagcggcca ctacatctac
     1621 aaacactgac gcttaaggga tcgtcccagc tccgaccaca aactatcaag tggtcggagc
     1681 tgggactttt actattttga ggagatgatc accgatagtt cacgcggtcc gtcaaattcg
     1741 accaacacca gtcgctgcca tgatcccagc tgcaggcggc ctttgatata gggcactacc
     1801 gcgctcggcc ccactagact agctagcaaa tgatccggcg catgcgccgg atcatgcgga
     1861 tgccgccagt caatgtttgg taccaggctg ttcaaaaagt ccaagaagtc atcgtctgtc
     1921 ccaggatcca gatccataat ggtcagcgcc gcggtggtgt gttgcacgaa tacactaagc
     1981 atgccttcat catccggcaa ggtcgccgcg acctgatcgg tgatgtcgat gacctgtcgt
     2041 ttgtgctcgg ttgatatgtg taacgtctgc ataaatcgcc tacaatagtc cgcgcacgta
     2101 agccgcttgg ccggcatgtt gccaatcgtc gactagaaca ctgactagcc tgacgccaag
     2161 cgtgaccgga ggatcccagc tttcgtcgat aatccgatcg tagtcctcag gttttaggcc
     2221 ggtaaggaag tcgctggtcg cttgctgtac agcgtcgaag tagcctttta gcagttcacc
     2281 gtcgacctgg acctggctga cttggtcaga atcctgccca taaccggtag cctgcttttc
     2341 aaacggcaag ccgaactcat cgtgccaacc gccgctaagc catacctgct cgcgatcggc
     2401 tagctcggcg atgtggtcat cttgtactcg gctcaagtgc cataccagcc atgatatcga
     2461 gttgccctta tcgtatggtc gataagccag ttcgtcggca gctgcatctt tgagcgcggt
     2521 atggactact ggcttcaagc ggtcaaaacc gtccgccata agctcatttg ttgtcataag
     2581 ccgatccttt cgctatatag ttactcaaaa cttcaccggt atagaaaccg aatcgtcgtt
     2641 ctcgggctca ccggatggat tgtcattctg tagcaccaag gtaccggttg aggtagccgg
     2701 tttgtcgaaa gttagactgg ccgcaaacgg cacatcctcg tcagtcatcc agtcaccctg
     2761 catcttggcg gtcgtctcgg ctatgacttg gccgttagcg tctttcaaga caactgggaa
     2821 cgaagcttcg aacgaccagt tacccggagc tttgccgata actccaagcg gactatcgac
     2881 ggtatcttga gccagcggtg agtagacggt tatatcgacg cccttggaag attcgaagat
     2941 gttcgtcggc cgacttcgat cggccggtag ctggtcggac tgattggatt gtttgtcgtt
     3001 ttgcaactgt tggacttggg cctgcagctg ctggttgcgc cgctccaagt cagcccgctc
     3061 cgatcgctgc cccaagaagt aataagccgc cccagcgacg gcgacgacca ataccagagc
     3121 cagtacccag gccgacagct tggctaaacg actaggcctt ggagctgagt agttcatgac
     3181 gttcatagta tcaccctcgt ttctatatat aactaaccat cc
//
LOCUS       DASSBW010000042         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_296203, whole genome shotgun sequence.
ACCESSION   DASSBW010000042 DASSBW010000000
VERSION     DASSBW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296203"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFG56_01195"
     CDS             <1..767
                     /locus_tag="VFG56_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6622529.1"
                     /translation="GGWISPISLRGKNSAINAYFQQEKGGGVFGKQQAYFAEFASANQ
                     ARQALLDKSCQINSPDRELNQKEVFGIKLPSDQNNHTCSRDKWPYKLGAFGGNAILVG
                     QIKDKLGSWLLLAGAVIAVIASLILMTMLGRIVADSRKETAIFRALGASRLSIMQVYL
                     TYTTFLILWVIGLSFALGLTLAFTFDYYLSPDFSLAAAVAFSVNNLDKKFGFIGLDWL
                     TLLEIFAIIIVTGLLASVMPILRSVRRNPIKDMREE"
     gene            complement(1076..1546)
                     /locus_tag="VFG56_01200"
     CDS             complement(1076..1546)
                     /locus_tag="VFG56_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6622530.1"
                     /translation="MAKEKTPKTNKLQESIDQLTTDLQRTRADFENYRKRTEADKESA
                     RTLGKTQAILKLLPVIDNIERAIGHMPEDLKDHKWAQGVAGLVKNLDKSLEGLDLKRI
                     DAKPGTVFDPALHEAIQMDEDSEGETEVIAEELQPGYLLAGQPIRHAMVRVKRQ"
     gene            complement(1628..1858)
                     /locus_tag="VFG56_01205"
     CDS             complement(1628..1858)
                     /locus_tag="VFG56_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622531.1"
                     /translation="MDSGAGWLNLIFPGWPIVIILAVICFIGVIVVVAAVAIGTGQEA
                     RNQEKYDKSDNKESEDSMLLADWKDSGEDKEK"
     gene            complement(1858..2565)
                     /locus_tag="VFG56_01210"
     CDS             complement(1858..2565)
                     /locus_tag="VFG56_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET6622532.1"
                     /translation="MTERQIKLLGVIIEQYAEVAAPVGSVTLAKLFGVSSATIRSEMA
                     ELERQGFIKQPHTSAGRIPTDKGYRFYVNRLTENAELAPTRIDRTARAIDARVAGYAD
                     KTNFAIRSAVDSLVDLTHNLGIATIGDQLYMSGMGNLFSQPEFSKASQVQAVARLLDN
                     LEPWLKEAAPNEPLNVFIGSENPIGRASEASLIISRFRSPYSDNSYIGVLGPTRQDYA
                     KVMRLVRHTGAMLEEVL"
BASE COUNT          679 a          685 c          662 g          784 t
ORIGIN      
        1 ggggtggctg gatatcgcca attagccttc ggggcaaaaa ctcggccatc aacgcctact
       61 tccaacaaga aaaaggcgga ggtgttttcg gcaagcaaca agcctacttc gccgaattcg
      121 cctcggccaa tcaggccagg caagccttgc ttgataaatc ctgtcagata aatagccctg
      181 accgtgagct taaccaaaaa gaggttttcg gtattaaatt acccagcgat caaaacaatc
      241 atacatgttc tagagataaa tggccctaca aactaggtgc tttcggcggc aatgctattt
      301 tagtaggcca gattaaagac aagcttggta gctggctatt gctggctggg gcggttatcg
      361 ctgtaatagc ctctttgatc ttgatgacca tgctcggtcg tatcgttgct gactcgcgaa
      421 aagaaacggc aatattccgg gccctaggag cttctaggct gtctatcatg caagtgtacc
      481 ttacctacac taccttcctg attttatggg tgatcggtct atcttttgcg ctagggctaa
      541 cgctggcatt tacctttgat tattacttaa gtccagattt tagcttggct gcggcagtcg
      601 ctttctcggt caataatcta gacaaaaagt ttggctttat cggtttagac tggctaacac
      661 tgctcgagat tttcgccatt atcattgtaa ccggtttgct agcttcggtt atgccgatcc
      721 tacgtagtgt ccgacgcaac ccgatcaaag atatgcgcga agaataagtt atttgacggt
      781 attctttgac tgagcttctt gttcgtcgag gttttgctga ccgagttggg catatacatc
      841 catctcaaat tgggctttct gcgactaatc ttttgggcac ggtaataagt ccagactggc
      901 cagctgatac ccacgccgac gaatccaatg actaagactg taattaagaa tggtagccta
      961 tccggaactt ggccggacat gcctgctcct ataattaatt ggaccgatgc aagcacccaa
     1021 ccgcaggata agatcattac cgcaaacaat gtaatatttc gtgcggtagc tcatcttact
     1081 gccgcttgac ccttaccata gcatggcgga tcggttgacc ggctagtagg tagccgggtt
     1141 ggagttcttc ggcgataact tcagtttccc cctccgaatc ttcgtccatc tggatggctt
     1201 cgtgaagagc cgggtcaaac acagtgccgg gcttggcatc gatgcgcttt aggtctaagc
     1261 cttctagcga cttatcgagg ttcttcacta ggccggcgac accttgagcc catttgtggt
     1321 cttttaggtc ttccggcatg tggccaatag cgcgttcgat gttgtcgatg acaggtagta
     1381 gttttaggat agcctgggtc ttacctagtg ttcgagcaga ctctttgtcg gcctccgtgc
     1441 gcttgcgata attttcgaag tcagcccgag ttctctgcaa atcggtagtc agctgatcga
     1501 ttgattcttg taacttattg gtcttcggtg tcttttcctt ggccatggtt tgaactttcc
     1561 tccctttcgt tcttcattct gaaaaatatg attattgcca ctagtaaagc aattagtatt
     1621 aatactttca tttttcttta tcttccccag aatctttcca gtcggctagt aacattgaat
     1681 cttcggattc tttattgtca cttttgtcat acttctcttg gttacgggcc tcctgcccag
     1741 ttccaatagc tacggcagcc accacaacta tgacaccaat aaaacaaatt acagccaaaa
     1801 ttataactat cggccagccg ggaaaaatta ggttcagcca gccggcacca ctatccacta
     1861 cagcacctct tctagcatag caccggtgtg acggactaaa cgcatgactt tggcatagtc
     1921 ctgccgggtc ggaccaagca cgccgatata ggaattgtct gagtacggcg agcggaaacg
     1981 gctgatgatg aggcttgctt ccgaagcccg accgatcgga ttttctgaac cgataaagac
     2041 attgagtggt tcgttcgggg cggcttcttt tagccaaggc tcgagattat ccagaagtct
     2101 ggcaacagcc tgaacttgtg aagctttaga aaattcaggc tgggaaaaca aattacccat
     2161 accactcatg tacaactgat caccgatagt agcgataccc aagttatgag tcagatcaac
     2221 caaggagtcg actgccgaac gaatagcaaa attggtcttg tcggcgtagc cggcgacacg
     2281 agcatcaatc gctctcgcag tgcgatcgat tcgagtcggc gccaactcgg cgttctcggt
     2341 caggcggttg acgtaaaaac gatagccttt atctgtcggg atacggccag cactggtatg
     2401 cggttgtttg ataaaacctt ggcgctccag ctcggccatt tcagaacgaa tcgtagcgct
     2461 agatacaccg aacagcttgg ctaaggtcac cgagcctact ggtgcggcaa cttcagcgta
     2521 ttgctcaatg atgacgccta gaagctttat ttgacgttct gtcatggttg tattttaact
     2581 tttctagcac tctaagtcaa tgagtgctag aactagttgg tttactagcc ctttctgttt
     2641 gtctataatt taacgtaatg caaactggta taactaagga ccagctaaag actattgctg
     2701 actggttggg tgccggatca atcaacctgt tcggtctacc ctttgccggt aaggatactc
     2761 acggtaccat actggccgag accttcgacg ccaccctgct cggcggtggc
//
LOCUS       DASSBW010000043         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1754457, whole genome shotgun sequence.
ACCESSION   DASSBW010000043 DASSBW010000000
VERSION     DASSBW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754457"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VFG56_01215"
     CDS             <1..1231
                     /locus_tag="VFG56_01215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6622533.1"
                     /translation="KQLDKQLADLGKMRILMLGWELPPHNSGGLGVACYHLAKALSRQ
                     GVAIDFILPKRLTGYPEVDFMNVLAVDDDQLPKPEPKTLPSAYEHYALGDVFALQQDY
                     IGYVSKLVAKQKPDVIHAHDWLTLEAGVAAKHLTGAPLIAHIHATEFDRAAGNRGNPI
                     IHDIEQTGLLMADRIVAVSNSTKNLIVERYQIPADKIEVIHNSAAGLTDITTAESVQY
                     SYLKAMQAEGYLVVATIGRLTMQKGLRFFLEAAAKANSRLERFVFVIAGDGEQRDELI
                     ELAADLGIADKVIFLGFIRGSAWRMMYEVADVFVMSSVSEPFGLTALEAAQCGAAISL
                     TKQSGVAEILTNALHYDYWDTDKLTDQLVNLAGSDSLHSELVTNARLEVSQMSWDQTA
                     TRLVGAYQQLRPGVTHV"
     gene            1224..2450
                     /locus_tag="VFG56_01220"
     CDS             1224..2450
                     /locus_tag="VFG56_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HET6622534.1"
                     /translation="MSKRQITLYLHVHQPYRLGRYTAFDTAERHDYFATEAGDIRQDN
                     EAIFKKVAQKSYWPMNRLLEKLLKDNPDFRLSLSITGTFIEQALEWDPELIKSFQRLT
                     STGRVEIVAETYHHSLAFFYSRMEFEKQVFMHREMVQRLFNQTPQVFRNTELAYNDEL
                     AAWADEAGYKAVLAEGWDKILGWRSPNYVYQPIGTSRLKLLLKNYRLSDDIAFRFSNR
                     AWADWPLTADKYLDWLDQLNDDQQLINLFMDYETFGEHQWADTGIFDFLEDFVSRWLN
                     DGNGFLTTTQAADLPVVDRVSASETVTWADNERDLTAWLGNRMQQEAMKFLYELEDDI
                     LRTHDQGLIADWRLMQTSDHSYYMCTKWWSDGDVHSYFSPYDSPYDAFMYFMNALRDL
                     RWRLLEHQKKAVSHGA"
     gene            2440..4383
                     /locus_tag="VFG56_01225"
     CDS             2440..4383
                     /locus_tag="VFG56_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET6622535.1"
                     /translation="MAREIVLSNGELFVGLNNYGEVHDFYFPYVGQENHAAAKELRHH
                     IGVFVDGRLSWLDDGSWEIRCRYHQDSMIGHVTAHQRDLQVSLEFDDCVDVSQAAFLR
                     NIHVINHANTHRNIRLFLHQAFVIGNSLDGDTAQYIPEEQAILHYKGHRAFVCAGRTS
                     DGQPQGQFSIGLHGIEGHDGTFRDAEDGELSGNSVEHGRVDSILRFSLDIDKHNSKRI
                     LYWVAAGTSQREAFKIHQRILKNGLMHYQSRTADHWRNWLQPALNICQKLDKDHRQEF
                     LTSLLVIKAHQDKRGSVIASGDTTMLNYARDAYAYCWPRDGAYALWPLLRLGYRDELI
                     HFFNFIRRSLDSDKGYLMHKYQPDGSLGSSWHSYLHDDQVGAPIQEDETALIVFLFGR
                     FIDKYDDKKLLVEFYPTMIKPMTDFMAGYIDEASNLPRPSYNLWEEIYQVNTYTTALT
                     AAALGEAADLADQLDKAEDALRYRTVSDNIRAAVDKLWNADTNYFNRGLWLKDNQLKA
                     DPLIDVASFYGVYMFGLMEADDHRLKTAFETLKSRLLVDGVAAPRYENDAYNRQNDSG
                     GGNPWPVTTLWLTQYAIETGDNEFTEAAINWVFDRLSYSGMLSEQYLSESMTPTSVGP
                     LVWSHAELVSTLLDLVGKTETEV"
     gene            4380..5033
                     /locus_tag="VFG56_01230"
     CDS             4380..5033
                     /locus_tag="VFG56_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622536.1"
                     /translation="MSLLGRLSPLGDLKTKQLIKSAVETYSLVYFGTVSQTDDEHATV
                     KGVTFSPRHIDHHYSVGTVEGFDVTLLQRTDSLSAPGKPIENYRWLLLRVSLKPSIKL
                     SHVLVDTAQYNQTYYANLFAKFSRLNSAKHCFEGPAYGEFSQAFHCYTTPDNLDHLAG
                     LIGPDTAQTILQHFPKFDVEWFDNQIIISSPEPLVSSRTIEMMLREALWLARYFESV"
BASE COUNT         1314 a         1283 c         1271 g         1308 t
ORIGIN      
        1 aaaacagctt gataaacagc tggctgactt aggtaaaatg cgaatcttga tgctaggctg
       61 ggaattaccg cctcacaaca gcggtggatt aggggtagct tgctaccatc tagccaaagc
      121 tctaagtcgc caaggcgtgg cgatagactt tatcttgccc aagcgattaa ccggctaccc
      181 tgaagtagac ttcatgaatg tcctagccgt agatgacgac caactgccta agcccgagcc
      241 gaaaacccta cccagtgcct acgaacacta tgcgctcggt gacgtgttcg cccttcagca
      301 ggactacatt ggttatgtca gcaaactggt agctaagcag aaacctgatg ttatacacgc
      361 tcatgattgg ttgacactgg aggctggcgt ggcggccaag catctcacgg gtgcgccact
      421 gatcgcccat atccatgcta cggaattcga tcgtgccgct ggcaaccgtg gcaatccgat
      481 catccacgac atagaacaaa ccggtctttt gatggctgat cgtatagttg ccgtatcgaa
      541 ctctaccaag aacctgattg tggagaggta tcaaatacca gccgacaaaa tcgaggtcat
      601 ccacaatagc gctgcggggc tgactgacat aactactgcg gagagtgttc agtatagcta
      661 tctgaaggct atgcaagccg aggggtattt ggtggtggct acgattggac gactaactat
      721 gcagaaagga ttgcggttct ttctagaagc tgccgctaaa gccaattcga ggctagagcg
      781 ctttgtcttt gttatcgctg gcgacggtga gcagcgcgat gaacttatcg aactggcggc
      841 cgacctcggt atcgctgaca aagttatatt cttaggattt atccgtggtt cagcttggcg
      901 gatgatgtac gaagtagccg atgtcttcgt tatgagctcg gtgtcagagc catttggctt
      961 aacggcctta gaggcggccc agtgcggtgc ggccattagc ctgactaaac aatccggtgt
     1021 agccgaaatc ctcaccaacg ctctgcatta tgactactgg gacaccgaca agctaactga
     1081 ccagttggtc aatctagccg gttcggatag cctgcatagc gagctggtca ccaatgctcg
     1141 actggaagtt agccaaatgt cttgggatca aaccgctacg cgtctagtcg gagcttatca
     1201 gcagcttaga cccggagtca ctcatgtcta agcgtcaaat aacgctttat cttcatgttc
     1261 atcagccgta tagactaggt cgttatacgg cctttgatac agccgaacgt cacgactatt
     1321 ttgctaccga agctggagat atccgtcagg acaacgaagc tatttttaaa aaagtcgctc
     1381 aaaagtctta ctggccgatg aaccggttac ttgaaaagtt actgaaagac aacccagact
     1441 ttcgcttaag tctcagtatc accggtactt tcatcgagca agctttagaa tgggaccctg
     1501 aacttatcaa aagcttccag cggctgactt cgactggtcg ggttgagatt gtcgccgaaa
     1561 cctatcacca tagtctagcc ttcttttata gtcggatgga atttgaaaaa caggttttca
     1621 tgcaccgtga gatggtgcag cgattattca atcagacacc acaagttttc cgcaacacgg
     1681 agctggctta caatgatgag ttggccgctt gggctgacga ggctggttac aaagccgttc
     1741 tggctgaagg ctgggataag atcctgggtt ggcgcagccc gaattacgtt taccagccta
     1801 tcggcaccag ccgactgaag ttgttactta agaattatcg attgagtgac gacatcgcct
     1861 ttcgtttttc caatagagct tgggccgact ggccgttaac cgctgacaaa tacctggact
     1921 ggctcgatca gcttaacgat gatcaacagc tgatcaatct cttcatggac tacgagacat
     1981 tcggtgaaca ccagtgggcc gataccggta tatttgattt ccttgaagat ttcgtaagcc
     2041 gctggctaaa tgatggtaat ggtttcttaa ctacaaccca agcagctgat ttaccggttg
     2101 tcgaccgagt cagcgcttca gaaaccgtta cttgggctga taacgaacgc gatctgacgg
     2161 cttggctggg taaccgtatg cagcaagaag ctatgaagtt tttgtacgag ctggaagatg
     2221 atatcctacg gactcacgac caggggctga tcgccgattg gcggctgatg caaacatctg
     2281 accacagcta ctacatgtgt accaaatggt ggagcgacgg cgacgttcat agctacttta
     2341 gcccctatga ctcgccttac gatgctttta tgtactttat gaatgccctg agggatttgc
     2401 gttggcgact tctagagcat cagaaaaagg cggtaagcca tggcgcgtga gatagttcta
     2461 tcaaacggcg aactattcgt cggcctcaat aattacggcg aagtccatga tttctatttt
     2521 ccgtatgtcg gtcaagaaaa ccacgctgca gccaaagaac tcaggcatca cataggcgtg
     2581 ttcgtcgacg gacgactcag ttggttagat gatggtagct gggaaataag atgccgctat
     2641 catcaggatt caatgattgg tcacgtcact gcccaccaac gcgacctaca agtcagcttg
     2701 gagtttgacg actgtgttga tgtcagtcag gccgcatttc tgcgcaatat ccatgtcatc
     2761 aatcacgcca atactcatcg taatatcagg ctattcttac accaagcttt tgttatcggc
     2821 aattcactcg acggtgacac cgctcaatac atacccgagg aacaggctat ccttcactac
     2881 aaaggtcacc gggctttcgt atgtgctggc cgaactagtg acggtcagcc acaaggtcag
     2941 ttttcgattg gcctgcatgg tatcgaaggt cacgatggca cattcaggga tgccgaggac
     3001 ggtgagttgt ctggtaacag tgttgaacat ggccgagttg actctatctt gcgctttagt
     3061 ctcgatatcg acaagcataa ctcaaaacgc atactttact gggttgctgc cggtacgtcg
     3121 caacgtgaag ctttcaagat tcaccaacgc attttaaaaa atggtctgat gcactatcaa
     3181 agtcgaaccg ccgatcactg gcgtaactgg ctgcaaccag ctctaaatat ctgccagaag
     3241 ctagataagg atcatcgaca agaattccta accagcctgc tagtcatcaa agcccaccaa
     3301 gataagcgtg gttcggttat tgctagcggc gatactacca tgctcaatta tgccagagat
     3361 gcctacgcct attgttggcc acgcgacggt gcttacgcac tatggccact actgcgactt
     3421 ggctatcgcg acgagttgat acacttcttt aatttcatcc gtcgcagcct agatagcgat
     3481 aaaggctacc tcatgcataa gtatcaaccc gacggtagtc tcggctctag ttggcactcc
     3541 tacttgcatg acgatcaggt tggagcacca atccaagagg atgaaaccgc cttgatcgtt
     3601 tttctgtttg gccggttcat agataagtat gatgataaga agttgttggt tgaattctat
     3661 ccgaccatga tcaaaccgat gaccgacttc atggccggtt atatcgacga agctagcaac
     3721 ttacctcggc catcgtacaa cttatgggaa gagatatacc aagttaatac ttacaccacc
     3781 gctcttaccg ccgcagctct tggcgaagcc gccgacctgg ccgaccaatt ggataaagcc
     3841 gaagatgctt tgcgttatcg gacggtatca gataacatcc gtgccgccgt cgacaaattg
     3901 tggaatgccg acaccaacta cttcaaccgc ggcctgtggc ttaaagacaa tcaactgaaa
     3961 gctgacccgc tgatagacgt agccagcttt tatggtgttt atatgttcgg gctaatggag
     4021 gccgatgacc accggcttaa gaccgccttt gagactctca agtcgcggct tttggtcgat
     4081 ggtgtagcgg cgcctcgtta cgagaatgac gcctataacc gccagaatga ctccggtggt
     4141 ggtaatccgt ggccggtgac gacattgtgg ctaacccagt acgctataga aactggtgac
     4201 aatgaattta ccgaggcggc catcaactgg gtttttgatc gtttaagcta cagcggcatg
     4261 ctgtctgagc agtacttgag cgaaagcatg acacctacgt cggtcgggcc tttagtctgg
     4321 agtcacgccg agctagtcag tacattactt gacctggttg ggaaaacgga gacagaagta
     4381 tgagcttact tggacgtctg tcgccactcg gcgaccttaa gaccaagcag ctgataaagt
     4441 cggctgttga aacttatagc ctagtctact ttggtaccgt ttcacaaacc gacgatgaac
     4501 acgccaccgt caagggtgtg actttctcac ctcgccacat tgatcatcac tattctgtcg
     4561 gtacggtaga gggattcgat gttacgctac tgcagcgcac cgacagtcta tcggcacccg
     4621 gaaagccaat tgagaattat cgctggttat tgctacgcgt cagtctcaag ccttcgatta
     4681 agctaagtca cgtcttagtg gataccgcgc aatacaacca aacatactac gccaatttgt
     4741 ttgccaaatt ctccaggcta aactcagcta aacactgttt tgaaggaccg gcatatggtg
     4801 agtttagcca agcttttcac tgctacacca caccggataa tcttgatcat ctagccggcc
     4861 tcatcggtcc cgataccgct cagacaattt tgcagcattt tcccaagttc gacgtggagt
     4921 ggtttgataa ccagatcatt atcagctcgc cagagccact ggtatcgtca cgcaccatcg
     4981 agatgatgct acgtgaagct ttgtggttgg cccgctattt cgagtccgta tagcaaaaac
     5041 cgcctttagt aaggcggttt ttgtgagact aaaagctcta acccttgcgt ggcttgactt
     5101 cgttacggcc aaggttcttc ttggtttcaa cgtaagtctc gtgccgacgt gtgatcgggt
     5161 cgtacttttt cagctt
//
LOCUS       DASSBW010000044         5870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_642376, whole genome shotgun sequence.
ACCESSION   DASSBW010000044 DASSBW010000000
VERSION     DASSBW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642376"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFG56_01235"
     CDS             <1..601
                     /locus_tag="VFG56_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET6622537.1"
                     /translation="RLNGLVGQPIKAVILDADKKANKLIFSEKEAVKEGLAERFNQLK
                     VGDSVKGVVTGVVDYGAFVNVDGIEGLIHISEISWERVNNPSDYVKVGDQIEAKIIAI
                     DKDRLSLSLKQLQEDPWLKEVDQFKSGDKVEGTVTRITPFGAFVQLSPAVEALVHVSE
                     LGSDAKDPEKVFKLNERKSFRVLDVDKDNHKISLSLKQK"
     gene            668..2428
                     /gene="infB"
                     /locus_tag="VFG56_01240"
     CDS             668..2428
                     /gene="infB"
                     /locus_tag="VFG56_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET6622538.1"
                     /translation="MPEKVLQVASTITVGELAEKVGLPVTKVVGELFKNGIVATVNQR
                     IDFETAQIIVDELELDVELQQKDETASPAETKRALTDKAQARPPIVAVMGHVDHGKTT
                     LLDAILGSKVAGGEAGGITQHISAYQAVRSDRPITFLDTPGHEAFSALRQHGAALTDV
                     VIIVVAADDGVKPQTVEAIKFAKSANARIIVAINKIDKETADPNRTKAELAENGLQTE
                     EWGGDVTMVEVSAKTGQNIDKLLDMVLLVSDLEELKAEVDIPAEGLVIESHMETGRGP
                     VVSLLVEHGELKLGQFVVAGSTYGKVRTEEDYAGNKLGLAGPATPLTMTGFKQVPAFG
                     DRFVVVKNEREARNQSSTNRQEADQAAAQANVTGSDLLKMMNKQRDTQEVNVIVKADV
                     QGSLTSLLDSLKLLATDEVNIRIVSSGVGNVTESDISRANGSSAVIYGFNVSLPSAVK
                     RSAARENVEVRIFDVIYQLIDDAKALMEDVLEPEVVETQIGQLKLLAVFRTTKSAVIC
                     GGEVLNGKVMPGVMARVKRGEEVLAETEVSHVQRQQQEAKEVFEGEQCGLELKTESKL
                     LLEAGDRLEFFTRELKQRSL"
     gene            2482..2907
                     /locus_tag="VFG56_01245"
     CDS             2482..2907
                     /locus_tag="VFG56_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HET6622539.1"
                     /translation="MFQLDDKFLSDVGLADLPAEQKQEFLNHTYLELERRVGEALSEG
                     LSEGQLSEFEAFIDRDEDKVRGWFTENLPEYNRLEDFQKLKASAPDGVAELDLLSEYG
                     SLKWLEINRPDYKQTVADEMAKLKQEIVDNHDAILGQSA"
     gene            complement(2911..3801)
                     /gene="fmt"
                     /locus_tag="VFG56_01250"
     CDS             complement(2911..3801)
                     /gene="fmt"
                     /locus_tag="VFG56_01250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET6622540.1"
                     /translation="MKFRKVIFFGTEDFSLPSLEALLEHDYQVVAVVTKPDSARGRSK
                     QLVEPAVKRLSKTYGIKVFQPTRLADINNELAELKADIGVLVSYGKILPERTLNLFSS
                     LGIVNVHPSLLPRYRGPSPIEAAIQNDDKTTGVSIMKLTLDMDAGPIYDQAELKLVGS
                     ETKPELYRRLAELGAERLVACLPAIISGELVAKPQLESDVTYTTLLSKDMAIVDPSTD
                     DAYAIERHTRAYLGFPKTKLQLQNNVVIITSAKVVDKKIDNQLVVECANKTYLLIEQL
                     VAPSGKSMTGQDYMRGYSLS"
     gene            complement(3767..4342)
                     /gene="def"
                     /locus_tag="VFG56_01255"
     CDS             complement(3767..4342)
                     /gene="def"
                     /locus_tag="VFG56_01255"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6622541.1"
                     /translation="MTDRTAIINLPNPHLRTPSKRVRVIGDEEKAVIDDMKAAALDWE
                     DSRPHEIAVALAAVQIDRLVRIVIVRGDFDNKEDRSFVVLINPEITKREGEIITDHEG
                     CLSVKDVYGLVPRHSKIRVRALDESGQQIRIKADGFLARILQHEIDHTNGICFVDHIE
                     NDKSAFYRLTEAGELESVSYDEVQKSHILRD"
     gene            complement(4382..>5870)
                     /gene="priA"
                     /locus_tag="VFG56_01260"
     CDS             complement(4382..>5870)
                     /gene="priA"
                     /locus_tag="VFG56_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET6622542.1"
                     /translation="TAIYLELARQTIKTGRSVIVIVPEIALTSQLVAEFTPDFPNLTV
                     THSNITPAKRRALWLKLLHDDSPQVVIGPRSALFSPLKDVGLIVIDECHEPSLSQDKS
                     PRYSALRAASVLAKASGSRLVLGSATPNVADYYLAKTTDPRSVIEIDKPARAGVRAAD
                     LTLVDLTKRANFSRNPVLSNQLIDAIQDSLDSNRQVILFHNRRGNASLTLCDNCGWSA
                     VCPRCFLPLTLHNDNFILRCHVCSHSEKVPLSCPDCSTVNVIHKGIGTKRIEDEVKRL
                     FKSASVARFDGDSQAEQTLDKVYQSIYDGDIDIIIGTQVVAKGLDLPKLGMVGVVQAD
                     AGLNLPDYYAEERTFQLLTQVIGRVGRTESLSQVIVQTYQPDHPAVRFGLKQGYHDFY
                     DYTLAKRFHDNFPPASFLLKLTCVYKTEAGAVRACRELAHKLRSTLPAGCQIFGPAPA
                     FYERQRDTYRWQILIKAKRRADLVQLASQVPAGKWQIDLDPTSLL"
BASE COUNT         1506 a         1359 c         1587 g         1418 t
ORIGIN      
        1 acgacttaac ggactggttg gtcagccgat caaagctgtt atcttggacg ctgacaagaa
       61 ggctaacaag ctaatattta gcgagaagga agctgttaaa gaaggcttag ctgagcgttt
      121 caatcaattg aaagtcggcg acagcgtcaa gggtgtagtt actggcgtag tggactacgg
      181 agccttcgtc aatgtcgacg gcatcgaagg tttgatccac atctcagaaa tttcctggga
      241 gcgggtcaat aacccaagcg attacgtcaa ggtcggcgat cagatcgaag ctaagatcat
      301 cgctatcgac aaagaccgct tgagcctaag tctcaagcag ttacaagaag acccatggct
      361 caaagaagtc gatcagttca agtctggcga caaggttgaa ggtacggtga cgcgaattac
      421 gccgtttggt gctttcgtcc agctcagccc ggccgtggaa gctctagtgc acgtatcgga
      481 gctaggctct gacgccaagg atcctgagaa agtatttaag ctaaacgagc gcaaatcttt
      541 ccgggttctc gacgtcgaca aagacaatca caagatctct ttgagcttga agcaaaaata
      601 gattattttc caagcaaaag taatcaaaag ttaatcccac gctgggaaag gaggtgacca
      661 agacagtatg cctgagaaag ttctacaagt tgccagtact atcacagtag gcgaattggc
      721 agaaaaagtc ggtttgccgg tcaccaaagt tgtcggtgag ttgtttaaaa acggcatcgt
      781 tgccaccgtt aaccagcgga ttgactttga aaccgctcaa atcattgtcg acgaactcga
      841 actcgacgtt gaattgcagc agaaagatga aaccgccagt ccggccgaga ccaagcgggc
      901 attgaccgac aaggcccaag ctcggccacc aatcgtcgct gttatgggtc atgtcgatca
      961 tggtaagact acactgctcg atgccatcct cggcagtaag gtagccggtg gcgaagccgg
     1021 tggtatcact cagcatatct ctgcctatca ggcggtcagg tctgatcgac cgattacttt
     1081 tttggacacg cctggtcacg aagccttttc ggccctgcgc cagcacggtg ccgctctgac
     1141 ggatgttgtc atcattgtcg tggcggccga tgacggtgta aaaccgcaga cggttgaggc
     1201 aatcaaattt gccaagtctg ccaacgccag aatcatcgtc gccatcaaca agattgacaa
     1261 agagactgcc gaccccaacc gaactaaggc agaactagcc gagaacggtt tgcaaaccga
     1321 ggagtggggc ggtgacgtta ctatggtgga agtcagcgcc aagactggcc agaacatcga
     1381 caagctgctc gatatggtac tactagtgtc cgacctggaa gagttaaaag ccgaggtaga
     1441 cataccggcc gagggtctgg ttatcgaatc ccacatggaa accggtcgtg gtccggtagt
     1501 atctttgctg gtcgagcacg gtgagctcaa actcggtcaa tttgtagtgg ccggctcaac
     1561 ttacggcaaa gttcggactg aagaagatta tgccggtaac aagttaggcc tggctggtcc
     1621 agctacgcct ctaactatga caggattcaa gcaagtgccg gctttcggtg accgcttcgt
     1681 tgtcgtcaag aatgagcgcg aagcccgcaa tcagtcgtcg accaaccgcc aagaagcaga
     1741 ccaggcagcc gcccaagcta atgtcaccgg ttcggatcta ctcaagatga tgaacaaaca
     1801 acgtgacacc caagaagtta acgtcatcgt caaggccgac gtccagggct ctctaacctc
     1861 gctactagac agtctgaagt tgttagctac cgacgaggtt aatatccgga ttgttagttc
     1921 tggtgttggt aatgttaccg agagtgatat atcgagagct aacggctcca gcgctgtgat
     1981 ttatggtttc aacgtcagtc taccgtcagc cgtcaagcga tcggcggcac gtgagaacgt
     2041 tgaggttaga atatttgacg ttatctacca attaatcgat gacgccaagg ctctgatgga
     2101 agatgtcttg gagccagaag tcgtcgagac tcaaatcgga caactcaagt tactagcggt
     2161 ctttaggacg actaagtcgg cggtgatttg tggcggtgaa gtactaaacg gcaaagtcat
     2221 gccaggagtt atggctcgag ttaagcgtgg tgaagaagtg ctagccgaga cagaagttag
     2281 ccatgttcag cgtcaacaac aggaagccaa agaagttttt gaaggcgaac agtgtggcct
     2341 ggaacttaaa actgagtcta aactactgct ggaagcaggc gatcggttgg aattcttcac
     2401 tcgcgagctg aagcagcgta gcctttaagc cgaatctata agtgctacaa taaaaccaaa
     2461 cagtaaccaa ggacagttaa catgttccaa ttagacgaca agtttttaag tgacgtcggt
     2521 ttggcggatt taccggctga acaaaaacag gaattcctaa atcatactta cctagagctg
     2581 gagcgtcgag taggcgaggc cctgtcggaa ggtctcagcg agggtcagct tagtgaattc
     2641 gaagcattca ttgatcgtga tgaagataaa gttcgtggtt ggtttaccga aaatttacct
     2701 gaatacaatc gactggagga tttccagaag ctaaaagcta gcgcgcccga tggtgtagct
     2761 gaactagatt tactcagcga gtacggttca ctgaaatggc tggagatcaa caggccggat
     2821 tacaagcaaa cggtggccga cgagatggcc aagttgaagc aagaaattgt cgacaatcac
     2881 gacgccatac ttggtcaatc ggcttaagaa tcagctcaaa ctatagccac gcatataatc
     2941 ttgcccggtc attgatttgc cgcttggcgc cactagctgt tcgatcaaaa ggtaagtttt
     3001 attggcgcat tccacgacta actggttatc gattttttta tcgactactt ttgcagaggt
     3061 tattataaca acgttatttt gaagttggag cttggtttta gggaagccca ggtaagctct
     3121 ggtgtggcgt tcaatagcat aagcatcatc tgttgatgga tcgactattg ccatatcttt
     3181 cgaaagtagg gttgtgtaag taacatcaga ttctagctga ggtttggcta ctagttcacc
     3241 gcttatgatg gcaggtaaac aagcgaccag gcgttcggcg ccgagctcag ccagacgccg
     3301 gtaaagctct ggtttagttt cggaaccaac cagctttagt tcggcttggt cataaatagg
     3361 accggcatcc atgtccaatg taagtttcat gatactgacg ccggtggttt tgtcatcgtt
     3421 ctgtatggcg gcttcgatcg gactcggtcc gcggtagcgt ggcagcagcg aagggtggac
     3481 attgacaatc ccaaggctac tgaataaatt tagtgttcgc tctggcagaa tcttcccgta
     3541 agataccagc acgcctatat cagcctttag ctcagccaac tcgttattta tgtcggctaa
     3601 tctcgttggt tgaaatactt tgataccgta agtcttggac agacgtttga cggctggctc
     3661 gactagctgc ttgcttcgtc cccgtgcgct atcaggcttg gtcaccacgg caacgacctg
     3721 gtaatcgtgc tccaacagag cttctaggct aggtaggctg aagtcttcag tcccgaagaa
     3781 tatgactttt ctgaacttca tcgtaactga cgctttctaa ctcaccagct tcagtgagcc
     3841 tataaaaggc ggacttatcg ttttcgatat ggtcgacgaa acaaattccg ttagtgtgat
     3901 cgatttcatg ctgtaagatg cgagccaaga aaccatcagc tttaatacgg atttgttgac
     3961 cggattcatc cagagcgcgg acgcgaatct tggaatgacg tggtacaaga ccatagacgt
     4021 ctttgacact taggcaacct tcgtggtcgg tgatgatttc accttcgcgt ttggtgatct
     4081 ctgggttgat taggactacg aagctgcgat cttctttatt gtcgaagtca ccccgaacga
     4141 tgacgatgcg gactagccga tcgatttgaa cggcggctag cgccacagca atctcgtgcg
     4201 gtctagaatc ttcccagtcc agggcggctg ccttcatgtc gtcgatgacg gctttttctt
     4261 cgtcaccgat aacacggaca cgcttggacg gcgtgcgcaa atgtggattt ggcagattga
     4321 taatagctgt tcggtcggtc ataaattccg tatcagtata acttaaacag ctgtttttaa
     4381 gctagagcag gctagtcgga tcgagatcga tttgccattt gccggccgga acctgagaag
     4441 ctagttggac tagatcggcg cgtcgtttag ctttgattag gatttgccag cgataagtgt
     4501 cgcgctggcg ctcatagaag gccggtgccg gcccgaagat ttggcaaccg gctggtaagg
     4561 tcgatcttag tttatgagcg agctcacgac aggcccggac ggcaccggct tcagtcttgt
     4621 aaacacaggt tagcttcagt aagaaactgg caggtggaaa gttgtcatgg aagcgcttgg
     4681 ccaaggtgta atcataaaaa tcatgataac cctgttttag accgaagcga accgccggat
     4741 ggtctggttg ataggtctga acaatcactt ggcttaaact ctcagtccga cctacgcgac
     4801 cgattacctg ggtgagcaat tggaaggttc gttcttcggc atagtaatcc ggcaggttca
     4861 ggccggcatc ggcctggacg acaccgacca tacctagctt tggtagatcc agccccttgg
     4921 ctactacctg agtaccgatg atgatatcaa tgtcaccgtc atagatcgac tgataaactt
     4981 tgtcgagtgt ttgctcagcc tggctatcgc cgtcaaagcg agcgacgcta gctgatttga
     5041 acaagcgctt aacttcgtct tcgatacgct tggtgccgat gcctttgtgt atgacattga
     5101 ccgtagagca gtccggacag gacaggggaa ccttttcgct atggctgcag acgtgacagc
     5161 gcagtatgaa gttatcgtta tgtagtgtca acggcaagaa acaccggggg cagacggcgc
     5221 tccagccaca gttatcgcat agcgttaggc tagcgttgcc acgacggttg tggaacaaga
     5281 tgacctggcg gttgctatct aacgagtctt gtatggcgtc gattaactgg ttggacaaaa
     5341 ccgggttgcg gctgaagttg gcccgcttag tcaaatccac tagcgttaaa tcggcagctc
     5401 gtactccggc acgagccggt ttgtctatct cgatcacact gcgaggatcg gtagttttag
     5461 ccagatagta gtcggctaca ttgggcgtgg cgctacctaa gaccaaacgg gagccactgg
     5521 ccttggccag cacggaggcg gcacgaagtg ccgagtagcg gggtgattta tcttgagaca
     5581 agctaggttc gtggcactcg tcgatgacga tcaggccgac atcttttagg ggactaaata
     5641 aggccgagcg tggtccgata accacctggg gagagtcgtc atgcagcagc ttgagccata
     5701 gagcccggcg tttggcggga gttatattgg aatgggtaac ggttaagttc ggaaaatcag
     5761 gtgtgaactc ggcgaccaat tgtgaggtca gggcgatctc cggtacgatg acgataaccg
     5821 atcgaccggt tttgattgtt tgccgggcta gctccaggta aatcgccgtc
//
LOCUS       DASSBW010000045         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_569524, whole genome shotgun sequence.
ACCESSION   DASSBW010000045 DASSBW010000000
VERSION     DASSBW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569524"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFG56_01265"
     CDS             complement(<1..307)
                     /locus_tag="VFG56_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HET6622543.1"
                     /translation="MSVRSSIYHTAESVSPGHPDKLCDQISDAILDAYLAQDKTARVA
                     VETVGGHGTIMVTGEISSSAQVEITPIVKRLSGKVELLERISLQSPEIARGVDQGGAG
                     "
     regulatory      complement(363..459)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(487..1122)
                     /locus_tag="VFG56_01270"
     CDS             complement(487..1122)
                     /locus_tag="VFG56_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6622544.1"
                     /translation="MKITKLVHSCLLVETPDRVGLIDPGSFSVEALSGISLTQLDDIL
                     ITHEHDDHMDIGVIKELLNDFPAVNIIAPDQAAQKLQAAGIETATDLLVDGVEAFDSP
                     HESVAPLFGQSPQEYGYHYLNQLSHPGDSHSFSETKSILALPVTAPWGATIKAINLGL
                     ELKPKYIVPIHDWHWNDQARASMYEKMTKVFADNDIEFIQAENGKAFEIDL"
     gene            complement(1190..1702)
                     /locus_tag="VFG56_01275"
     CDS             complement(1190..1702)
                     /locus_tag="VFG56_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622545.1"
                     /translation="MGFWTVVGRLIAGKPAFEVPNNTRQTDRTDVAPAPVNGRIDHGR
                     RLDSSGRKIIPEAQLVRSEYHVSGEHLQVSAFIRNNSRVQIEIDKVNIFGRLYQVNRW
                     LNPGEEHETVVYRGPHLKSDAYKMGELYYKDCQSGDYFCALHQVDYRREPDGSFLPHG
                     FHLIHPIKDI"
     gene            complement(1759..2187)
                     /locus_tag="VFG56_01280"
     CDS             complement(1759..2187)
                     /locus_tag="VFG56_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET6622546.1"
                     /translation="MDLASNVQLDFVVALLIALLCGTIIGVERELKHKPAGLKTQILV
                     VTASMLFAFLSHEYGDGDPTRIAAQIVSGVGFLGAGLILKSDGHHVDNVTTAASIWFA
                     AAVGLSIGFGFYFAALITAIVGALVLRLPRLSDGPKPKDD"
     gene            2255..3049
                     /locus_tag="VFG56_01285"
     CDS             2255..3049
                     /locus_tag="VFG56_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6622547.1"
                     /translation="MQTIKLSNKLEMPIIGFGTWRITNDQAGVDLVKQALELGYRMID
                     TAAMYDNEAAVGQAVRDSGLSRSEIFVVTKVWVDDFGYAATLKAYESSLKRLGLDYAD
                     LYLIHHPTTDSESYIQSWRALERLYRERRVKSIGVSNFWPNNLEKLRQRDLMTPHVNQ
                     IKISPFLQQLPTREYCLRHNIAIQSYSPLDRGGSLLDDKLIRKLAAKYNRSPAQIVLR
                     WHIDSGLVPLPKSANLDRIAENHDIDFKIKDDDMVAIAKLDQGPRA"
     gene            complement(3046..>3370)
                     /locus_tag="VFG56_01290"
     CDS             complement(3046..>3370)
                     /locus_tag="VFG56_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622548.1"
                     /translation="IAVIAHKFSDGLSTVSLMTVNRNRSSQVKFMLGLNAIAPLVGAA
                     STLLFTASETSLVLYLGFFAGFLLYIGASEILPEAHSKQSAYSTILFTVAGVAFMFGV
                     TQLAG"
BASE COUNT          901 a          872 c          786 g          811 t
ORIGIN      
        1 cgccggcgcc gccctgatcg acgccccgag cgatttccgg gctctgtagg gagatacgct
       61 caagcagttc taccttaccg gacagacgtt tgactattgg agttatttcg acctgagccg
      121 aactgctgat ctcaccggta accattatcg tgccatgccc gccgacggtc tcaacggcga
      181 ccctggctgt tttatcctgc gccaaatagg cgtccaagat agcgtcagag atctggtcac
      241 atagtttatc cggatgaccc ggactaacgc tttcagccgt gtggtaaatt gaactacgta
      301 cagacataaa aatactcctt tcatctgctc cgcaggagtt caaaaggagt acaatattta
      361 ctcgatcatc tgccccgtac gggctggaat tagcaccttg gggttaccag gttgctgacg
      421 gatcatcggg ccagttccct actcgtctct tgataattag ttgttaacgc cactaattat
      481 agcgcctcat aggtcgatct caaaagcttt gccgttttcg gcttggatga actcgatgtc
      541 attgtcggca aaaactttgg tcatcttttc atacatacta gctcgggcct gatcgttcca
      601 gtgccaatca tggatcggga cgatgtactt aggcttcaac tcaagcccca ggtttatagc
      661 cttgatagtg gcgccccatg gtgccgtgac cggtaaagcc agtatcgact tggtttcaga
      721 aaaactgtga ctatcacctg gatgcgacag ctggtttaaa taatggtatc cgtactcttg
      781 cggcgattga ccaaacagcg gcgccactga ttcatgtgga gaatcaaatg cctcgactcc
      841 atcgactaac agatccgtcg cggtctctat accggcggct tgtagttttt gtgcggcttg
      901 atccggtgcg atgatattga cagccggaaa atcatttaac agttccttga tgacacctat
      961 atccatatga tcatcgtgtt catgggtaat caaaatatca tcaagctggg taagactgat
     1021 gccgcttagc gcctcaacac tgaagcttcc tggatcaata aggccgactc gatctggtgt
     1081 ttcgactagc agacaagagt ggactaactt agtgattttc atggtgacct ccttttgacc
     1141 ttaggctcat taaatacaaa cggcggagac caagccgtga aatttatcac tatatatctt
     1201 tgatcggatg aatcaagtga aaaccgtgcg gcaagaaact gccatccggc tctcgtcgat
     1261 agtcgacttg atgcagggcg cagaagtagt cgcctgattg acagtctttg tagtacagct
     1321 cgcccatctt ataggcatcg ctttttagat gcggaccacg atataccacg gtttcatgct
     1381 cttcaccggg gtttagccaa cgattaacct ggtaaagtcg gccgaatata ttgaccttat
     1441 caatttcaat ttgcactcta gagttattgc gaataaacgc cgatacttgc agatgttcac
     1501 cgctcacgtg gtactcactc cgtaccagct gcgcctcagg tattattttt cgaccgctag
     1561 agtcaagtcg acgaccatgg tcgatcctgc cgttaacagg cgcaggggct acgtctgttc
     1621 gatcggtttg cctggtatta tttggcactt caaaggccgg cttacctgca atcaatcttc
     1681 cgacgactgt ccagaatccc atagctagat tgtaacacgg gcaaatctac accatgagag
     1741 cccggcacga caagcaagct agtcgtcttt tggcttgggg ccgtcgctta aacgcggcaa
     1801 ccgaagcacc aaagcaccaa cgatagcggt gataagagca gcaaagtaaa agccaaaacc
     1861 gatgctaaga ccgacggcgg cggcaaacca gatgctagcg gccgtagtca cgttatcgac
     1921 gtgatggccg tcagacttta ggatcaggcc ggctccgaga aagccgacgc ccgaaactat
     1981 ctgagccgca atccgggttg ggtcgccatc gccatactcg tgcgacaaaa aagcaaacag
     2041 catcgacgcc gttactacaa gtatttgggt ctttaggccg gccggcttgt gcttaagttc
     2101 tcgctcgaca ccgataattg tgccgcaaag cagcgcgatc agcagagcga caacaaaatc
     2161 aagctgtacg ttagaagcta aatccatcat tacccttaag gttacaccaa atcgacttga
     2221 ttgaaaacgt tataattgga ggagaagagg gcttatgcag acgattaaac tcagtaacaa
     2281 actggagatg ccgatcatcg gtttcggcac ttggcggatc accaacgatc aagctggtgt
     2341 tgatctggtc aaacaggctc tagagcttgg ctatcgcatg atcgatactg cagccatgta
     2401 cgacaacgaa gcggccgtcg gccaagccgt ccgcgacagc ggcctaagca gaagcgagat
     2461 ctttgtggtc accaaggtgt gggttgatga cttcggctat gccgcaactc ttaaagctta
     2521 cgaatccagc ctgaaacgcc taggacttga ttatgccgac ctttacctta tccaccaccc
     2581 caccaccgac agtgagtcct atattcaaag ctggcgcgca ttagaacgct tgtaccggga
     2641 gcgccgagtc aaatccatcg gcgtatcgaa tttctggcca aacaatctag aaaaattacg
     2701 ccagagagac ctaatgacac ctcatgtaaa ccaaatcaaa atcagtcctt ttttgcagca
     2761 actacctact cgagagtatt gcctgcgtca caatatcgcc atacaaagct acagcccgtt
     2821 agaccgcggc ggttcacttc tagacgacaa attgattcgc aaattggccg ccaaatataa
     2881 ccgttcacca gcccagatcg tactcagatg gcatatagac agcggcctgg ttccactccc
     2941 gaaatcggct aatctagacc gtatagccga gaatcatgac atagacttca agattaaaga
     3001 cgacgatatg gtagctatcg ccaaactaga tcaaggacct agggcttaac cggctaattg
     3061 cgtcacgccg aacataaacg ctactccggc aactgtgaac aaaatcgtcg aataagccga
     3121 ctgcttacta tgggcttctg gtaatatctc gctggcacct atatacagca agaagccggc
     3181 aaaaaatcca agatatagca ccaaactggt ttccgaagct gtaaataaca gagttgaagc
     3241 tgcgccaact aggggcgcga tagcgtttaa tcccagcatg aatttaactt ggcttgagcg
     3301 gttgcgatta accgtcatca gactaaccgt acttaatcca tcactaaatt tgtgggcaat
     3361 aaccgcgatt
//
LOCUS       DASSBW010000046         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1710404, whole genome shotgun sequence.
ACCESSION   DASSBW010000046 DASSBW010000000
VERSION     DASSBW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710404"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFG56_01295"
     CDS             <1..402
                     /locus_tag="VFG56_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622549.1"
                     /translation="RQEDSGWAPKQTTTTMNNYQTRRNRNFEARQERMWQRGQNTVAF
                     KPSAKLGPVTHTIIVALMVAVLGLIYLTQVTRTSSYAYQLNDAQTTHDKLVAEYQDLK
                     VENARLQSLKTVQDSDVAKAMTEPKSTSFAN"
     gene            424..2190
                     /locus_tag="VFG56_01300"
     CDS             424..2190
                     /locus_tag="VFG56_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6622550.1"
                     /translation="MFLSVISYLRRQPRANILALALLVVMSIFVVRLFYLQVVRHDYY
                     LAIANANQMSKFTIPATRGEIYALSRGQPVPLVLNKTVYLMFADPSEVDDPEELKSAV
                     RQVAGGDLIEGSLGSLDDDSLHYVVLARAINGDQAKLLKSKQIAGLGFKETTERVYPE
                     GGLAAQTLGFVDANGQGQYGVEQSLNGQLAGRTGLLKTVTDVRNIPLTIGDNNIRQPA
                     VDGQDIVLSIDRNIQAKVEAVLAKGLKHANAHHGSVVVMDPNTGKVMAMANLPTFDPA
                     NYAKTTDYADFSNDVVSSPYEPGSVMKTLSVGIGIASGAISKNSTYDNTNKIQVDGET
                     INNSAVDPRIPDATVTDILHYSLNTGVVWTLKQMGGGEVNDKARQTLYNYYHDNFRLG
                     RVTGIEQAGEQAGIIYKPNEGFGRNVRYANMTFGQAMSVTMIQFAAAFSAEINGGTYY
                     QPSVVAGWLEDDGTLKPKPAKSLGQSISRKDSLTMRRLIHKATMAGTFGSVAPDGYNI
                     GGKTGTAQKIDPETGKYSMEYNQTTGSYVGFGGTDTPRYVIMVRVMDPNIPGYAGTEA
                     AAPIFNDISNWMINYLRLPPNK"
     gene            2200..3246
                     /gene="mraY"
                     /locus_tag="VFG56_01305"
     CDS             2200..3246
                     /gene="mraY"
                     /locus_tag="VFG56_01305"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET6622551.1"
                     /translation="MHNQTIVLSQVTDQLVKISILALLAFLLAMALTPIYTFFAYRYR
                     FWKKQRQTDTSGKQLKVFTKLHAEKFKRHIPTMAGMIFVVAIAIVTLLFNLDREQTWL
                     PLAALIGGALVGLIDDVINLKSDGRGVAGMRSSLKFTLITFVGLVGAWFFFDKLGYDQ
                     VMVPFIGLVTIGWWIVPLFVLAVVSTGNAVNISDGLDGLSGGLIVSAYSFFAAIALLQ
                     GNFGIAGFCFTVVGAMLSYLWFNIHPARFFMGDVGSFALGTSLGVVAMLTNSILLLPI
                     IGLVFVVETGSSLLQILSKRFLGRRIFISAPLHQHFEAQGWPETKVTMRFWVIGGVVG
                     FLGLLLALAGGSVK"
     gene            3276..>4375
                     /locus_tag="VFG56_01310"
     CDS             3276..>4375
                     /locus_tag="VFG56_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET6622552.1"
                     /translation="MAQTTGQLIRRHQPDYQIFMFAGILILVGLIVLFAISPARVALI
                     NASGQHIDESQFMFKQCLNLVIGVMAFVVAANLPFSFWKKHTNKFLLGGLAACVLLAI
                     LSIGQTAPALCINGACRWYNLGFASFQPAEFLKFGVLLFLTGFLARRAAEGQLDSVKN
                     CLWPLAVVVGLAGFLIVVAQKDMGTGLTLMGMVIVMIFAAGMRMKTIGLILAALFAVF
                     VLTTVTSPHRMERIVTFFHPEAASQEDSYHVEQATISIGSGGLVGRGLDNSRQAFGYL
                     PEAVNDSIFAIFGETFGFVGLLAVLGLFSALLMRLLKVFRHLADPMMRLAVVGVFGLI
                     ISHVIVNVGAMTGIIPLTGVTLPFISFGGTSLL"
BASE COUNT         1025 a         1089 c         1129 g         1132 t
ORIGIN      
        1 cggcaggaag attccggctg ggcaccaaaa caaacaacca ccactatgaa caattatcaa
       61 accagacgaa atcgtaattt tgaggcgcgc caagaacgca tgtggcaacg cggtcaaaac
      121 accgtagcgt ttaaaccatc ggcgaaatta gggccggtta ctcacactat catcgtagcc
      181 ttgatggtcg cagttctcgg tttgatttat ctaacccagg taactcgaac cagcagctac
      241 gcttatcagc tcaatgacgc tcaaaccact cacgataaac tagtggccga atatcaagac
      301 ctgaaggtcg agaatgccag gctacagtca cttaagaccg tacaagattc agacgtagcc
      361 aaggccatga ccgagcccaa atcgacgtca tttgccaact gattcgttat aatttattag
      421 agtatgtttt tatcagttat cagctatttg cgtcgtcaac ctcgagctaa tatcctggct
      481 ttggcgctgc tggtagtcat gtctattttt gtagtccgac tgttttacct tcaggtagtc
      541 cgccatgatt attatctggc catcgccaac gccaaccaga tgagtaaatt taccatacca
      601 gccactcgtg gtgagattta tgccctaagt cgtggtcagc ctgtgccgct agtgctcaac
      661 aagacggttt acctgatgtt tgctgatcct tcagaagtcg atgatcctga agaattgaaa
      721 tccgccgttc gccaagtcgc cggcggcgat ttgattgagg gtagtttagg tagtttagac
      781 gacgatagtt tgcactatgt ggtactggcc agggcaatca atggtgatca ggctaagctg
      841 cttaagtcca aacagatagc cggtctaggc tttaaagaga cgaccgaacg ggtttatccg
      901 gaaggcggcc tagcggctca gactcttggc tttgtcgatg ccaacggcca gggtcaatac
      961 ggtgtcgaac aatcgctaaa cgggcagctg gccggccgaa ccggattgtt aaaaaccgtc
     1021 accgacgtca ggaacattcc gttgaccatt ggggacaaca acatccgcca gccggcggtc
     1081 gacggccaag acatagtttt atcaattgat cgcaatatcc aggctaaagt tgaagcggtc
     1141 ctagccaaag gcttaaaaca tgccaacgct caccacggca gcgtggtcgt gatggacccc
     1201 aataccggta aggtcatggc gatggctaat ttacctacct ttgatccggc taattacgcc
     1261 aagacgactg attatgctga tttttctaac gatgtggtgt ctagccccta tgaaccgggc
     1321 tcggtcatga agacccttag tgtcggtatc ggtatcgcta gcggagctat atccaagaac
     1381 tccacctacg acaataccaa caagattcaa gtcgatggcg agactatcaa taacagcgcc
     1441 gtcgatccgc gcataccaga cgctacagtg accgatatcc tgcattattc acttaacacc
     1501 ggtgtagttt ggacactgaa gcaaatgggt ggtggtgaag ttaacgataa ggctcgacag
     1561 acgttataca actattatca cgacaatttt cgactaggta gagtgacggg catcgagcaa
     1621 gccggcgagc aggccgggat aatctacaaa cccaacgaag gttttggccg aaatgtccgc
     1681 tacgccaaca tgactttcgg tcaggctatg agtgtgacga tgattcagtt cgcggcggct
     1741 tttagcgccg agatcaatgg aggtacatac tatcagccca gtgtagtcgc gggctggctg
     1801 gaagatgacg gcacgctcaa accgaaaccg gctaagtcac tcggtcaatc gatcagccgc
     1861 aaggattcgt taactatgcg gagacttatt cataaggcca ccatggcagg tacttttggg
     1921 tcggtcgcgc cagacggcta caatatcggc ggcaagaccg gcacggctca gaagattgat
     1981 cccgagacgg gtaaatacag tatggagtac aaccaaacta ccggtagcta tgtcggcttt
     2041 ggcgggacag acaccccccg ttatgtcata atggtgaggg ttatggaccc taatataccg
     2101 ggttacgccg gtaccgaagc ggccgcgccg atattcaatg acatctccaa ctggatgatc
     2161 aattacttaa ggttaccgcc aaacaaataa gaggaaaaca tgcacaacca gaccatcgtt
     2221 ttaagccaag taaccgacca gttggtcaaa atatcgatcc tagccctcct agctttcttg
     2281 ctagccatgg ctttgacacc gatctacacc ttcttcgctt accgctaccg cttctggaag
     2341 aagcagcgcc agaccgacac cagcggcaag caattaaaag tctttacgaa attacacgcc
     2401 gagaaattta agcgccatat cccaactatg gccgggatga ttttcgtcgt ggcaatcgct
     2461 atcgtcacgt tactgtttaa cctcgaccgt gaacagacat ggctaccgct ggcggcactt
     2521 atcggcggtg cgcttgtcgg tctaatcgat gacgtgatta acctcaagag tgacggtcgt
     2581 ggggtagccg gcatgagaag tagcctcaag tttaccttga ttacttttgt cggtctagtc
     2641 ggtgcctggt tcttcttcga taagctaggt tacgaccagg tcatggtgcc gtttatcgga
     2701 ctggttacca tcggttggtg gatcgtaccg ctgtttgtat tggcggtagt atcgaccggt
     2761 aacgctgtca acatcagcga tgggctcgat ggcttatcgg gcggcttgat tgtgtcggct
     2821 tatagctttt ttgccgccat agctctactg cagggtaact tcggcatagc cggtttctgc
     2881 ttcacggtag tcggtgctat gctgagctac ttgtggttca atatccatcc agcccgtttc
     2941 tttatgggtg acgtcggtag ttttgcgctc ggtacttctc tcggcgtagt agctatgcta
     3001 actaactcga ttttgctact accgatcatc ggactagtct tcgtagtcga gaccggctct
     3061 agcttgctgc agattttgag caaaaggttt ttgggccgtc gcatttttat ctcggcgccg
     3121 ctgcatcagc atttcgaagc tcagggctgg cctgagacca aagtaaccat gcgtttttgg
     3181 gtaatcggtg gcgtggtcgg tttccttgga ctactactgg cgctagccgg cgggagcgtt
     3241 aagtgaggtc gagcgtgaat cgctacgcca gtcgcttggc acaaaccacc ggccaactga
     3301 ttcgccggca tcaacctgac tatcagattt ttatgtttgc cggtattctg atcctggttg
     3361 gcttgatcgt cctattcgcc atcagtccag ctcgtgtggc actaatcaac gctagtggtc
     3421 aacacatcga cgagagccag tttatgttta agcagtgcct caacctagtt atcggtgtga
     3481 tggcttttgt ggtcgccgct aatttgccgt ttagcttttg gaaaaagcat accaataaat
     3541 tcttacttgg cggcttggcg gcctgtgtct tactagctat cttatcgatc ggccaaacgg
     3601 ctccggcttt atgtatcaac ggtgcctgtc ggtggtataa tcttggcttt gccagctttc
     3661 agccggccga gtttttgaaa ttcggcgtct tacttttctt aaccggtttc ttagctcgac
     3721 gggccgccga aggccagctc gatagtgtaa agaactgctt gtggccacta gcggtcgttg
     3781 tcggtttggc tgggttcttg atagtggtag ctcaaaaaga catgggcacc ggcctgacac
     3841 tgatgggcat ggtgatagtg atgattttcg ctgccggcat gcggatgaag accataggac
     3901 tcattttggc agccttgttt gccgtattcg tgttgactac cgtaacgtcg ccgcaccgga
     3961 tggagcggat agttactttc tttcacccgg aggcagccag ccaagaggac agctaccacg
     4021 tggagcaggc gactatctct atcggcagcg ggggattagt cggtcgcggc ctagacaata
     4081 gccgccaggc tttcggctac ttaccggaag ccgtcaacga ttcgatcttt gcgatcttcg
     4141 gtgaaacgtt tggttttgtc ggcttgctag cggtattggg actgtttagt gcactactga
     4201 tgcgtctact taaggttttt cgacacttgg ccgacccgat gatgcgctta gctgtagtcg
     4261 gagttttcgg actaatcatc tctcatgtca tcgtcaatgt cggtgccatg actggcatca
     4321 ttccgttgac tggcgtgacc ttgccgttta tcagttttgg cggcaccagc ttgct
//
LOCUS       DASSBW010000047         6040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1450024, whole genome shotgun sequence.
ACCESSION   DASSBW010000047 DASSBW010000000
VERSION     DASSBW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450024"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFG56_01315"
     CDS             <1..504
                     /locus_tag="VFG56_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6622553.1"
                     /translation="IIGLHARGAMLPVSWIFGKSSSSLFYYDAVTDAKVLSVSRQQLL
                     DTVMSRPELSKQMFDYLSGEHTASLLRINGLEQSRAIEKVGFMLYYLVFRYGKDEGKG
                     RFSLTIKLSQLMLANLVGLTRESTTKSLLSLKSKGVISYKNSNYRIDKTRLENFLGED
                     SFRDLKL"
     gene            complement(501..896)
                     /locus_tag="VFG56_01320"
     CDS             complement(501..896)
                     /locus_tag="VFG56_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6622554.1"
                     /translation="MKVRYDESMKQANILIVEDDRDLNEAYRLILEKDGHKVQTAFDG
                     QQALERLKDGPDPDIILLDLRMPVMDGIEFLKEYKPDDHNKTTVVVFSNYDAQKEVDE
                     AYELGAHRYVLKARAAPKELQRIVADIIG"
     gene            complement(898..2019)
                     /locus_tag="VFG56_01325"
     CDS             complement(898..2019)
                     /locus_tag="VFG56_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6622555.1"
                     /translation="MDRRTGLSLVKVLLVDDDEDEYVIIKAMLDKIPDMRFELDWCAS
                     FVEAKKVIAQTKHDAYLVDYRLGGHTGLELLEFAKPQRRNQPFILLTGVGDRDIAWKS
                     MKLAAADYLVKGSFNEELLGRILSYAIERKQIEQQRLDHLLELNKSKDEFISIASHQL
                     RTPATGVKQYLGMLLEGYFGELTPKQKQMLEKAYHNNDRQLRIVSDLLKVAQVDAGKI
                     NLHRAEVELDKLVKSVVDDHSGRFASRGQKLIFKPKPEGERAMIDEEAVRMVLDNLLD
                     NASKYSPEKTTTTVSVKRSGGMACIDVKDQGVGISEDSRSRLFQKFSRIDNPRSREES
                     GTGLGLYWAKKIIDEHKGEIKVTSEEGNGSRFTVCLPKA"
     gene            complement(1997..2467)
                     /locus_tag="VFG56_01330"
     CDS             complement(1997..2467)
                     /locus_tag="VFG56_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6622556.1"
                     /translation="MTKATQEPITIVMADDDDDDFLLTQKALKQSKLLNKLVRVENGE
                     ELMDYLLGQGTFKGIECDRPGVILLDLNMPKKDGRETLKEIKAHTELRDIPVVIFTTS
                     KAEEDIYRSYQLGVNSFITKPVTFDNLISTMTALGKYWFEIVELPKANGQKNRS"
     gene            complement(2421..4289)
                     /locus_tag="VFG56_01335"
     CDS             complement(2421..4289)
                     /locus_tag="VFG56_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6622557.1"
                     /translation="MNRPKNQSKINFEELFHASPFAGLLIEADGKEFTVLAQNKAHAN
                     LTKTTPEDIVGQPLLKAFPGISGKRDEERVQSDPTMKAFRSVMDSGKLEVIDEIRYDI
                     VDKDGHVFRRFWKFDISPIKGDDDKVSCIYLTVQDVTGSKIQSDNLRLLSQAGRQFSA
                     SLKYTDTLHNIASLVVPDVADWCTIDLLRDDGQIEQVVLIHKDPKKIAWAKELRAKQG
                     APDPNDPIGVPHVIRTGKPEHMPVITDDMLKEAAENDEQLKLTRSLNFSSVMILPLKI
                     DGRTIGAMSLVSTEGRRHYDEDDLNVGQSLADRAALAVYNAKLYQSAREELTQRNQLQ
                     SRIESINESLEDMVKQRTLELEAVNRGLEKEIDKRELAEAELIRSNDELQNFAYVASH
                     DLQEPLRKIQAFGNLLDSEYRAELGDGKDYLDRMQNAASRMSVLIDDLLSFSRVSTGT
                     AQPARVDLNDVVGDVISDLESLISDTSGKVTVDKLPVVTADDTHMRQLFQNLISNAIK
                     FRRDDVKPQVKIDCKSAGKFYEITVKDNGIGFDEKYLDRIFTVFQRLHGREAYSGTGI
                     GLAVCQKIVQRYGGIITAKSRPQHGSTFIIRLPKQAKRSVNRDQSHARADHHSNGR"
     gene            complement(4482..5408)
                     /locus_tag="VFG56_01340"
     CDS             complement(4482..5408)
                     /locus_tag="VFG56_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622558.1"
                     /translation="MSEFDRSESMADKANLANELAEASAERHKYVLDKIETAPPIDPD
                     SVDLTVKNPDRLRAGLKSELEYSFAVESEVMRNVKELHHLFPEMPDTDRLFVDELWRP
                     HEQMHGLAIGRLLDEIGLEEPPMDLETISPKMRVLAALGRSAAIADVERLIYYLTGAS
                     TEKAATMLYDSMTKTLKGLGETAIENTLIGQIKRQEPIHLAYYRKAAEELTGQLSPWQ
                     LHLTRVLRSKSFNLVGVTNRQRQQTMGQIISDNIGYGDEQLEPYAEKIGIVDHQLLWA
                     ERQGMKVPPYILKALKDSVEVHKEATENSSLA"
     gene            complement(5529..5807)
                     /locus_tag="VFG56_01345"
     CDS             complement(5529..5807)
                     /locus_tag="VFG56_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET6622559.1"
                     /translation="MDMLLDIILWILLGALAGWIASKVMNTDEQMGAFANIVVGIIGA
                     FIGGIIVRGLTGSSVDGLSIWGLVVAVIGAIILLAIVKAFRGQSHSRA"
BASE COUNT         1429 a         1497 c         1431 g         1683 t
ORIGIN      
        1 atcatcggct tgcacgcccg cggtgccatg cttccggtca gttggatctt cggtaaatct
       61 tcaagcagct tgttttatta tgatgccgtg accgacgcca aggttttgag tgtcagccgc
      121 caacaattac tagatacggt catgagtcga cccgagctgt caaagcaaat gtttgactac
      181 ctatccggtg aacacacagc ttcactacta aggattaacg gtctggagca gtctcgagcc
      241 atcgaaaaag tcggtttcat gctctactat ctagtttttc gctacggcaa agacgagggt
      301 aagggtcgct tcagcctgac tatcaaactc agccaactaa tgctggccaa tttggtcggc
      361 ctgacacggg aaagtaccac aaagagccta ctatcgctaa agagtaaggg tgttatcagt
      421 tacaagaact cgaactatcg tatagataaa actcgcctag agaactttct aggcgaggac
      481 agcttccgtg atcttaaact ttagcctatg atatcggcga cgatccgctg cagctcttta
      541 ggtgcggccc gcgctttaag aacatagcga tgagcaccta gttcgtaggc ctcatcaact
      601 tctttttggg cgtcgtagtt gctgaaaacc acgaccgtag tcttgttgtg atcgtccggc
      661 ttatattctt tcaagaattc gatgccatcc atcaccggca tacgcaggtc gagcaggatg
      721 atatcgggat ccggaccatc ttttaggcgc tctagcgcct gctgaccatc aaaagcagtt
      781 tgaactttgt gaccatcctt ttccaggata agccggtaag cctcgtttag gtcacggtca
      841 tcttctacga ttaggatatt ggcttgcttc atagattcat catacctcac tttcatttta
      901 agccttaggt aggcaaaccg tgaaacggct gccattacct tcctccgatg taactttaat
      961 ctcgcctttg tgttcatcga tgatcttctt ggcccaatag agacccaatc cggtgcccga
     1021 ctcttcgcgc gaacgggggt tatcgatgcg tgaaaacttt tgaaacagtc gtgatcgact
     1081 atcctcactt atgccgaccc cttggtcctt gacatcgata caggccatgc cgccggatcg
     1141 cttgacgctg acggtcgtag tggtcttttc tggcgaatac tttgaggcat tgtcgagtag
     1201 attgtcgaga accatccgaa ccgcctcttc atcgatcatg gcccgctcgc cttcaggttt
     1261 gggcttgaag atgagcttct gtccacgact agcaaaacga cccgagtggt cgtcgaccac
     1321 cgacttgact aacttgtcga gttcgacttc ggctcggtgt agattgattt taccggcatc
     1381 gacttgggcg actttcaaga gatcggatac gattctcaac tgacggtcat tgttatgata
     1441 ggctttctcc aacatctgct tttgctttgg tgtaagttcg ccgaaataac cctccagcaa
     1501 cataccgagg tattgtttga caccggtggc cggtgtgcgt agttggtgtg aggcgataga
     1561 gataaactca tctttactct tgttaagctc cagtaggtga tcgagtcgtt gttgctcgat
     1621 ctgtttgcgt tcgatggcgt aggacaagat gcgtccgagc agctcttcgt tgaacgaacc
     1681 tttgacaaga tagtcggcgg cagccagttt catcgatttc catgcgatgt cacggtctcc
     1741 aacaccggtc agcaagatga aaggctggtt gcgacgctgt ggtttggcga actccaatag
     1801 ttcaaggccg gtgtggccgc cgagccgata gtcaactaag taagcgtcgt gcttagtctg
     1861 agcgatgact tttttggcct ccacgaaaga agcgcaccag tccaactcga aacgcatgtc
     1921 cggaatctta tccagcatgg ccttgatgat gacgtattcg tcttcatcat cgtcgaccag
     1981 tagtaccttg actagactaa gaccggttct tctgtccatt ggccttaggt aattcaacga
     2041 tttcgaacca gtatttgccg agggccgtca tggtggagat cagattgtcg aatgtcaccg
     2101 gtttggtgat aaaagagtta actcccagct gatacgagcg gtagatgtct tcttcggcct
     2161 tggatgtagt aaagataacc accgggatgt cacgaagttc ggtgtgggcc ttgatttctt
     2221 tgagtgtctc acggccatct tttttcggca tattgagatc gagtaagatg acaccgggtc
     2281 gatcacattc gatacccttg aaagtacctt gacccagaag ataatccatc agttcctcgc
     2341 cgttctcgac ccggactagc ttattgagga gcttgctttg ttttaatgct ttttgggtca
     2401 gcaaaaaatc gtcgtcgtca tcatcggcca ttactatggt gatcggctct tgcgtggctt
     2461 tggtcacggt tgacgctccg tttcgcctgt ttaggtaacc tgataataaa ggtggagcca
     2521 tgttgaggac ggctcttggc tgtaattata ccaccatagc gctgaacaat cttctggcaa
     2581 acagccagac cgataccagt gccggagtaa gcttcacgac catgcagacg ctggaaaacg
     2641 gtgaagatgc gatccagata tttttcatcg aaaccgatgc cattgtcttt gacggtaatc
     2701 tcatagaatt tgccggcaga tttgcaatcg attttgacct gtggtttgac atcatcacga
     2761 cggaacttga tggcatttga gatcaggttt tgaaaaagtt gacgcatatg ggtgtcgtcg
     2821 gccgtaacaa ccggtagctt gtcgaccgtt accttgccgc tagtgtcgct gatcaatgat
     2881 tcgaggtcac tgataacatc cccgaccacg tcattcagat cgactctcgc cggctgcgca
     2941 gtgccggttg atacacgcga gaaggacagt aagtcgtcga tcagcacgct cattcgagag
     3001 gcggcatttt gcatgcgatc aagataatcc ttgccgtcac ctagctcagc ccggtattcg
     3061 gaatcaagta ggttaccgaa agcttgaatt ttgcgcaagg gttcttgtaa gtcgtgagaa
     3121 gccacgtaag cgaagttttg cagctcatcg ttgctcctga tcagctcagc ttcggccagt
     3181 tcccgcttgt ctatctcttt ttctaggccg cgattaacag cttccagctc cagggtgcgc
     3241 tgcttgacca tatcttccaa gctttcattg atagattcga tccggctctg tagttggttg
     3301 cgctgtgtca gctcttcacg agccgactgg taaagcttag cgttgtagac cgccagagcc
     3361 gcccggtctg ccaagctctg gccgacattc agatcatctt cgtcgtaatg gcgacgtcct
     3421 tcagtagata ccaatgacat ggcgccgata gtccgtccat ctatcttcaa cggcaagatc
     3481 ataaccgagc tgaagttcaa actacgagtc agcttgagct gttcgtcatt ttcggccgct
     3541 tccttcagca tatcatcggt aatgactggc atatgctcag gctttccagt ccgaattaca
     3601 tgtggcacac ctatcggatc gttcgggtca ggcgcgcctt gcttagccct taattctttg
     3661 gcccaagcga ttttcttggg gtccttatgg atcaatacaa cttgttcgat ttgaccatcg
     3721 tcccgcagta aatcgatggt acaccagtcg gctacatccg gtactacaag cgaagcgata
     3781 ttgtgcagag tatcagtgta tttaagcgaa gccgaaaatt gacggccggc ctggctgagc
     3841 agtcgtaagt tgtcggactg aatcttcgat cctgtgacat cttgaaccgt caaatatata
     3901 cagctgacct tatcgtcatc accctttatt ggcgagatat caaacttcca gaaacgtcta
     3961 aacacatggc cgtctttatc aactatgtca tagcggatct catcaattac ttccagctta
     4021 cccgagtcca taacggatct gaacgccttc atcgtcggat ccgattggac tctttcttca
     4081 tcgcgcttcc cggaaatccc cgggaaggct tttagtaacg gttgaccaac gatatcctca
     4141 ggagtagtct tagtcaaatt ggcgtgagcc ttgttttgag ctagtaccgt aaattcctta
     4201 ccgtcagctt cgatgagaag gcccgcaaac ggagaggcat gaaaaagctc ttcaaagttg
     4261 attttagact gatttttagg tcgattcatg cggtgccttc ctccgtaagc cacttacttt
     4321 atttttggtc tctttaattg agtcttcatc tagcatagaa gcctcttccc taaaaacatg
     4381 tgaaaaagat caccaaattg ttacttcaag ggtgattttc gttctaaaaa tgtgttcgaa
     4441 tctttcttcg gtcgttaaat cgtagtcaaa attaataaaa cctaagccag ggaagagttt
     4501 tccgtcgcct ccttgtgtac ttcaacactg tccttaagag cttttaggat ataaggcggc
     4561 accttcatgc cctgtcgttc cgcccacaga agctggtgat caactatgcc gattttctcg
     4621 gcataaggct ctagctgctc gtcgccgtag ccgatgttgt cggaaattat ctggcccata
     4681 gtctgctgcc gctgtcgatt ggttacaccg acaagattga aagatttgga tcgtagcacc
     4741 cgtgttaggt gtagctgcca tggagatagt tgtcccgtca gctcctcagc cgccttgcgg
     4801 taatacgcaa gatgtatagg ttcttgacgc ttgatctgtc cgatcagagt attctcgata
     4861 gctgtttcac caagaccttt gagtgtctta gtcatcgaat catacagcat ggtggcggct
     4921 ttctcagtgc ttgctccagt caagtagtag atcaggcgct cgacatcggc tatagcagca
     4981 ctacggccca aagctgctag gactctcatc ttggggctga tagtttctaa atccataggt
     5041 ggctcttcta ggccaatctc atctaacagc ctgccgatag ccagaccgtg catctgttcg
     5101 tgcggccgcc atagttcgtc gacaaataaa cgatcggtat ccggcatttc aggaaaaagg
     5161 tgatgtagct ctttgacatt gcgcattact tcactctcca cagcaaacga atattctagc
     5221 tcgcttttta agccggcgcg cagccggtct ggattcttga ctgtcagatc cacactatcc
     5281 ggatcgatag gcggtgcggt ctcgattttg tctaagacat acttatggcg ttcagcgcta
     5341 gcttcggcta gttcattagc taggttagcc ttatcagcca tggattcact tctatcaaat
     5401 tcgctcatag ctaaaaacta tatcacaaat agttaagtag gttaagtatt tgtgatatta
     5461 aaaatcttca tacgtaaata gaggccggtt aagacctcta tttacagttc tgtcggtggg
     5521 cctagcgctt acgctcggct gtgcgactgg ccacggaagg ccttgacgat agccaacaag
     5581 ataatcgcgc cgataacagc cacgactagt ccccagatgc tgaggccgtc aactgacgat
     5641 ccggtcagtc cgcggactat gatgccacct atgaaggcgc cgatgatacc gaccacgata
     5701 ttggcaaacg cgcccatttg ttcgtcggta ttcataactt ttgaggcgat ccaaccggct
     5761 aatgcgccta gtaggatcca cagaatgata tctagtagca tatccatctc ctttagttta
     5821 agttgtgtct taacattaac tcttaagact aactaaagat gtaataaata ttacgagttt
     5881 tactaaatgc gcttgaaatc tacgtcagga tagatgtctt tggtggtctc gtcaatttcg
     5941 tatttgagct gtgggaatgg cgtaccttcg cgggccgata cgccttctag gctccagaaa
     6001 acccgttcgc tataacccaa gccgcaagcc ggtggcatgc
//
LOCUS       DASSBW010000048         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1560267, whole genome shotgun sequence.
ACCESSION   DASSBW010000048 DASSBW010000000
VERSION     DASSBW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560267"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1159)
                     /locus_tag="VFG56_01350"
     CDS             complement(113..1159)
                     /locus_tag="VFG56_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET6622560.1"
                     /translation="MNNELTPVVDTTPDLKSVVAGILDVIEKDREKEIVARRFGLNAP
                     KETLEQIGELLGITRERVRQLEKAILIRLKIAAESGQLDLERAEKFIIKELHEMGRAA
                     RLSDLAIKINPEATETDKAHLAFISTLSPKLTVLDENDNYHHGVGISEHFDDKNFKRG
                     VDEVVKTVKTHGQAIKIDNLHDKLSYEHPDHVRAVASLSKHLANLKDDWGLTKWPAVN
                     PKNIRDKIYVILQEKASPMHFSDIARAIKDSAFKRKDVTTQAIHNELIKDKRFVLVGR
                     GIYALKEWGFKQGTVSDVIRSVLKKNGGPMHRDEIVRAVLKERQVKETTILLNLQSKP
                     EFKRVAKATYDLAQ"
     gene            complement(1342..>2979)
                     /locus_tag="VFG56_01355"
     CDS             complement(1342..>2979)
                     /locus_tag="VFG56_01355"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HET6622561.1"
                     /translation="KVKRVLEDYSNSNFSVADISKKPGTRNPSAPFTTSTLQQEASNR
                     LGYSPRQTMQLAQRLYESGHITYMRTDSLNLSKQSLAQAASYIEKNFGKDYHQFRTFK
                     AKTSGAQEAHEAIRPTDIAKLEAGADQQAKKLYGLIWRRTLASQMAPAALERTTVSIA
                     ASDSSEKLVAKGEIVTFDGYLKVYGRSRDDILLPKLSKGDPLQLISAEAVESLSRGPA
                     RYTEASLVKQLEEMGIGRPSTYASTLTTIQSRGYVERGDVEGEDRQVTKLSLIDGRLS
                     ETIETISYGKDSNKLVPTDTGLVVTDFLTKHFAEVMDYDFTRRIEDELDQVAEDHKPG
                     VEVLDDFYKPFHKLVEKSADVSRAEASQARHIGDDPKSGRPIYARFGRYGPMLQMGEK
                     DDEAKPEFAPMPSGAKIETVTLEQALEMFKLPRLVGTTDDGQEITANIGRFGPYVKIG
                     SLFVSIKPEDPHTITLDKAKELYADKLKKEAEKNIADFGSIKVLNGRFGPYVTDGKKN
                     AKIPKDTDPKSLDEAAAKELLDKAPASKKRRVIRRKK"
BASE COUNT          677 a          685 c          755 g          862 t
ORIGIN      
        1 gctatcggca accaaataaa ccactgggtc agcaggccaa atataaagga gagactagag
       61 cccaacatac tacctatatt gtcgcttaaa aaagcgtgct ttacaaataa acttactggg
      121 ccaagtcata ggtcgcttta gcgacgcgct tgaactccgg tttactctga agattcagca
      181 aaatggtggt ttctttgacc tggcgctctt taaggacggc ccggacgatt tcatcacgat
      241 gcattggtcc gccattcttc ttgagaaccg agcggataac gtcggaaacg gtaccttgct
      301 tgaagcccca ctctttcagg gcgtagatac cgcggccaac caggacaaag cgtttgtctt
      361 tgatcaattc attgtgaata gcctgtgtag tgacatcttt gcgcttgaat gcactgtctt
      421 tgatagctcg agcgatgtcg gaaaagtgca ttgggctagc cttctcctgt aggatgacgt
      481 aaatcttgtc gcggatattc ttcgggttaa cggccggcca cttggtcaag ccccagtcgt
      541 ctttcaggtt agctagatgt ttacttaagg aagcgaccgc ccgaacgtgg tcagggtgtt
      601 catagcttag tttgtcatgc aagttatcga tcttgatagc ttggccatga gttttgacgg
      661 tcttgactac ttcatcgaca ccgcgcttaa agttcttgtc atcgaagtgt tcagatatac
      721 cgacaccatg gtgatagttg tcgttctcgt caagaacggt cagtttaggc gataaggttg
      781 aaatgaaagc caagtgggct ttgtcggtct cagtcgcctc aggattaatc ttgatagcca
      841 gatcgcttaa gcgagcggcg cggcccattt cgtgcagttc tttgatgatg aatttttcag
      901 ctcgttccaa gtctaactgg ccgctttcgg cggcaatttt taagcggatt aggatggcct
      961 tttccagttg acgtacccgt tcgcgagtaa tgcctagtaa ttcaccaatt tgctccaagg
     1021 tctcttttgg agcgtttagg ccaaagcgac gggctacgat ttccttctca cggtcctttt
     1081 caatgacatc gagaatacca gccaccacac tttttaagtc aggggtggtg tccacaaccg
     1141 gggttagctc gttgttcatt aaaaaatcgt cctctcttga cgctatgttt tagcgttcag
     1201 gccttttgtt aaattatatt atagaaccta ctttagaaaa agtcaaatac aacttctaat
     1261 gacttaatta tagcacaaaa tagacttaaa atataatgag tggtttaagg cttttattag
     1321 tttaggtaat atgacttaaa cttatttttt acgccggata acccgtcttt tcttactggc
     1381 aggggccttg tcgagtaatt ccttggcggc agcttcgtcg agtgatttcg gatcggtatc
     1441 cttagggatc ttggcgtttt tcttgccgtc ggtgacgtaa ggaccgaacc gaccgtttag
     1501 cactttgata ctaccgaagt cagcgatgtt tttctcagct tctttcttga gcttgtcggc
     1561 gtagagctcc ttggccttat ctagtgtgat ggtgtgtgga tcttctggtt tgatcgacac
     1621 aaataagctg ccgatcttta cgtaaggacc gaaccggccg atgttagcgg taatttcttg
     1681 accatcatcg gtcgtaccga ccaatcgagg tagcttgaac atctcgaggg cctgctctaa
     1741 ggtgacggtc tcaatcttgg ccccgctggg catgggtgcg aattcgggct tagcttcatc
     1801 gtctttctcg cccatttgca gcatcgggcc ataacggcca aagcgggcat aaattggtcg
     1861 accggattta gggtcgtcac cgatgtggcg ggcttgagaa gcttcagccc tggagacatc
     1921 ggcacttttt tcgactagct tgtggaaggg cttatagaaa tcatcaagga cttcgacgcc
     1981 aggcttatga tcttcggcaa cttggtcgag ctcatcctcg atacgtctag tgaaatcgta
     2041 gtccatgact tcggcgaagt gcttagtcaa aaaatctgtc accactagac cggtgtcggt
     2101 tggtactagt ttgttagagt ccttgccgta actgatggtt tctatggtct cagacagccg
     2161 accatcaatc aggcttaact tggtcacttg ccggtcttca ccttctacat cgccacgttc
     2221 gacataacca cgggattgaa tagttgtcag tgtcgaggcg taagtactcg gacgaccgat
     2281 gcccatttct tctagctgtt tgactagact ggcttcagta tagcgagccg gtccacgact
     2341 gagtgactct acggcttcgg cagagatgag ttgtagcgga tcacctttgc ttagtttcgg
     2401 caaaaggatg tcgtcacggg aacgcccata gactttcagg tatccgtcaa aggtgacgat
     2461 ctcacctttg gcaaccagct tttcactgga gtcgctggcg gcaatactga cagtagttcg
     2521 ttcgagggcg gcgggcgcca tctggctggc caaggtacgc cgccaaatca gaccgtagag
     2581 cttcttagcc tgttggtcgg cgccggcttc tagtttggcg atgtcggtcg gacgaatggc
     2641 ttcgtgggct tcttgagcac cggaagtctt ggccttgaaa gttctaaact gatgataatc
     2701 tttaccaaaa tttttctcga tgtagctggc ggcttgcgcg agtgactgct tggataaatt
     2761 gagtgaatcg gttcgcatgt aagtgatatg accggattca tatagacgct gggccagctg
     2821 catggtctga cgcggactat agcccaggcg gttcgaggct tcttgttgca aggtcgacgt
     2881 ggtaaaagga gccgatggat tacgtgtacc tggctttttg gagatatcgg ccacactaaa
     2941 gttgctattt gaataatctt ctaagactcg ttttacttt
//
LOCUS       DASSBW010000049         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_544284, whole genome shotgun sequence.
ACCESSION   DASSBW010000049 DASSBW010000000
VERSION     DASSBW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544284"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /gene="gatC"
                     /locus_tag="VFG56_01360"
     CDS             complement(<1..255)
                     /gene="gatC"
                     /locus_tag="VFG56_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET6622562.1"
                     /translation="MKSLSFDDVKKLADLSQLELSAEEAASLQADLAKILDYVDQLKS
                     VDTDAVEPTYQVNYSGTVTREDQTVDYGVSPEAMLDLAPEA"
     gene            complement(297..1316)
                     /locus_tag="VFG56_01365"
     CDS             complement(297..1316)
                     /locus_tag="VFG56_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622563.1"
                     /translation="MQPEQQNLNSPQTTNQPESSASTAPKTGRSLPHWLNRTGMYGFE
                     QFMALVGLLTTAWVLDYGWFALFNTFSGSAFGSFASQMALFIVVGMLVWLPVTMFFFL
                     RMRGQSVAQPEVNHSHLAKFFNAAYLVIAIMAAIGFAFTAIYSLLNSVVNLDQAVGET
                     ALRVTVPAVLSLLTELILVAAIMRPKGLFSRGKFAVWFSLLSLGVVVTLFILSAGNIR
                     SYAADERVVNDFQEIHSSIQNYYDDNNRLPNRLDGSLTELSQEVSNRLGDYQYVPKTA
                     GKYQLCADFHKASRGYDSVQPDDSYRTYVRSSHPDGHYCYNLKVYGVYSSDSFDLYRP
                     SKSLY"
     gene            complement(1341..>2423)
                     /locus_tag="VFG56_01370"
     CDS             complement(1341..>2423)
                     /locus_tag="VFG56_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET6622564.1"
                     /translation="QTGFVKGIENYSRYLAGRKAGEQPSTLLDYFPDDFLLLIDESHM
                     TIPQVRGMYNGDRARKEVLVEHGFRLPSALDNRPLTFSEFERHINQVIYISATPGPYE
                     LERTPEPVEQVIRPTGLLDPKIEIRPVEGQVDDLIAEIRDRTAQGERVLVTTLTKRMA
                     EDLTTYLQDLDIKTAYLHSDIDTLERSDILRDLRLGVYDVLVGINLLREGLDLPEVSL
                     VAIIDADKEGFLRSSQALIQTIGRAARHERGRVVMYADHITDSMRRAIDETERRRTIQ
                     EEYNRKNGITPVSVAKAVGEGLRAVIPMSEDDKKPKLNLNKIPKDEYPQLIKDLTSQM
                     ELAAANLEFEKAAELRDTIDNIKRRA"
BASE COUNT          591 a          598 c          614 g          620 t
ORIGIN      
        1 ggcttcgggt gccaaatcca acatcgcttc cggtgaaacg ccgtaatcaa ccgtttggtc
       61 ttcccgggta acggtaccag aataattgac ctggtaggtt ggctcgacgg catcggtgtc
      121 aaccgatttt aattgatcga cgtagtcaag gatcttggcc aggtcggcct ggagactagc
      181 cgcttcttca gccgacagtt ccagctgtga caaatcggcc aattttttga cgtcatcaaa
      241 agacagtgat ttcattgagc ttaattataa cagataaaac agcggctcat aaacgcttag
      301 tacaacgatt tactcggccg atataggtca aagctgtcag acgaatagac accgtagact
      361 tttaggttat agcagtagtg accgtcaggg tggctagacc ttacgtaagt acgatagctg
      421 tcgtcaggct gaactgaatc gtaaccgcgc gaagccttgt ggaaatcagc acacagttgg
      481 tatttgccag cggtctttgg cacatactga taatcgccta gtcgatttga aacttcttgg
      541 ctcagttccg ttaaactacc gtccaaacga ttgggtagac ggttgttatc gtcgtaataa
      601 ttctggatcg aggaatggat ctcttggaag tcattgacta cgcgttcatc ggcggcatag
      661 gagcgtatat tgccagccga caagataaac agggtgacga caacacctag gctaagtagg
      721 ctgaaccaaa ctgcaaattt accacgactg aacaaccctt tcggcctcat gatagccgcc
      781 accaggatta gttcggtcaa cagtgacaag accgccggaa ccgtcactct gagggcagtc
      841 tcgccgacag cctgatcaag attcaccacg gaattcaaca ggctatagat agcagtaaag
      901 gcaaagccaa tagctgccat gatggcgata actaaataag cggcgttaaa gaactttgca
      961 agatgactat ggttgacttc cggctgggcc actgactgcc cacgcatcct taggaagaaa
     1021 aacatcgtta ccggtagcca aaccaacatg ccgacgacga taaatagcgc catctggctg
     1081 gcgaagctgc cgaaggctga accactgaag gtgttaaaca aggcaaacca accgtaatca
     1141 agcacccacg ctgtagtcag caaaccgact agcgccatga actgctcaaa gccgtacata
     1201 ccggtgcgat taagccagtg aggcaggctt cgtccggttt taggggccgt cgacgcacta
     1261 gattctggct gatttgtagt ttgtgggctg tttagatttt gctgttcagg ctgcatttga
     1321 acctcgtttt ttatttttac ttatgctcgt cttttgatgt tgtcaattgt atcacgaagc
     1381 tcggcggctt tctcaaactc cagattggca gctgccagtt ccatctgcga agtcagatct
     1441 ttgataagtt gtgggtattc gtctttgggg atcttattta gatttagctt tggcttcttg
     1501 tcgtcttcac tcatcggtat aaccgctctt aggccctcgc cgacggcttt agcgacactg
     1561 accggtgtaa taccattctt gcggttatat tcctcctgga ttgtccgacg acgttcggtt
     1621 tcgtcgatag ctcggcgcat cgagtcggtg atatggtcgg cgtacatgac aacccggcct
     1681 ctttcatgac gggctgcccg accgatagtc tggatcaagg cctgcgagct tcttaagaaa
     1741 ccttccttgt cggcgtcgat gatggcgaca aggctaactt ccggcaggtc aagaccttca
     1801 cgtagcaagt tgatgccgac cagcacgtca taaacaccca acctaagatc acgcaggatg
     1861 tcgctgcgct ctagagtatc gatatcggaa tgcagataag cggttttgat atccaggtct
     1921 tgcaggtagg tagtcagatc ttcagccatt cgcttagtca gggtagttac cagtacccgt
     1981 tcgccttggg cggtccgatc gcgtatttcg gcaatcagat catcgacttg tccttcgacc
     2041 ggtcgaatct ctatctttgg atccagcaga ccggtcggcc taatcacctg ttcgaccggt
     2101 tcgggtgttc gctctaattc ataagggccg ggtgtggccg agatatagat gacttggttg
     2161 atatgacgct caaattccga aaaggttaac ggtcggttat cgagagcact aggcaggcgg
     2221 aagccgtgtt cgaccaaaac ttctttcctg gctcggtcgc cgttatacat acctctcacc
     2281 tgaggtattg tcatatgtga ttcgtcgatc agcagcaaga aatcgtccgg aaaatagtca
     2341 agcaacgtcg acggctgttc gccggctttg cggccggcta ggtagcgcga atagttctcg
     2401 atgcctttga cgaagccggt ctg
//
LOCUS       DASSBW010000050         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_19277, whole genome shotgun sequence.
ACCESSION   DASSBW010000050 DASSBW010000000
VERSION     DASSBW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19277"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /gene="rsmD"
                     /locus_tag="VFG56_01375"
     CDS             complement(<1..448)
                     /gene="rsmD"
                     /locus_tag="VFG56_01375"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET6622565.1"
                     /translation="MKVRLISGVYGGRFLDAPKSTRTHPMSERVRASLFNQLGDVSGK
                     TVLDAFAGTGALGFEALSRGAASATFVERDKLAQKVLTENIQSLGVSDKAKLIKSGVS
                     GWTDTTTGQSFDLIFVDPPYHDLQLSTVAKLVKYLQPNGLMILSFPG"
     gene            complement(445..1266)
                     /locus_tag="VFG56_01380"
     CDS             complement(445..1266)
                     /locus_tag="VFG56_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622566.1"
                     /translation="MYSGTTFRNKSGQVIGAHQKIDRVARRQLKPVLPAGVNFPTVKQ
                     ILHFEGNNGPDGIKRKSPGKDELWHFIDPKNPNKGQLLDIIDDHLHNLVEALVADNLE
                     RAAFEAAWLAHAIVDGLTPAHHYPLEEKLEILRGETMDTRVGVKQKLLIPGKTRRELI
                     KNNWQFWGSKGVMTTHFLFELGVATTIAGLKMSYARPTADQLIRVEDNGLRAVFLDSL
                     VRVHHLKMYEKFHKTGWTRGLAGDTRAKLAPTIVRTVTLAWYLAATQAAKKRSKL"
     gene            complement(1349..>2380)
                     /locus_tag="VFG56_01385"
     CDS             complement(1349..>2380)
                     /locus_tag="VFG56_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET6622567.1"
                     /translation="SVGLLTGNVKGKARTNLYQSIEDGGSAVVVGTHALLTDKFKFHK
                     LGLVVIDEQHRFGVRQRQTLFDKSEKMPHVLSMTATPIPRSLQLTVYGELDISLIRQK
                     PVGRKPILTKITSPNSRAQLYATIDQQITDGAQVYVVCPLIDEGKDGEQKSVEAEFKR
                     LKNSIFKNRRIGLMHGKLPADQKAEVMQDFIDHRIDILVTTTVVEVGVDVPNASVMII
                     EGADRFGLAQLHQLRGRVGRSDTQSYCYLIPSTSAKPSQRLKEMEKSNDGFYLAEKDL
                     ELRGPGEIYGRAQHGQLRLAVANLADTGLLKKAQAAARDLVAQDFDLLQYDRLSREVD
                     RYQRLTTLN"
BASE COUNT          520 a          628 c          622 g          610 t
ORIGIN      
        1 tacctggaaa tgataatatc ataagcccgt tgggttgcaa atatttaact agcttggcaa
       61 ctgtggataa ctgcagatca tggtatggcg ggtcgacaaa tatcagatcg aaactctggc
      121 ccgtggtcgt atcggtccag ccgctaacgc ctgacttgat cagcttggct ttatcgctca
      181 caccgagtga ttggatgttc tcggtcagga ctttttgggc cagtttgtcg cgctcgacaa
      241 aagtagccga agcggcaccg cgactcaagg cctcgaagcc taatgcacct gtaccggcaa
      301 aagcgtcgag gacggtctta cctgatacgt caccaagttg gttgaacaaa ctggcgcgta
      361 cccgttcgct catcgggtgg gtccgggtcg acttgggtgc atccaagaac cggccaccgt
      421 acaccccgga gatcaagcgg actttcatag tttgctccgc tttttggcag cttgggtagc
      481 cgctaggtac caagccagtg tcaccgtccg aacgatggtt ggtgccagct tggcccgagt
      541 gtcgccagcc agccccctag tccagcctgt cttatggaat ttctcataca ttttgaggtg
      601 gtgcactcgg acaagactat caagaaaaac tgctctcaga ccattgtctt cgacccggat
      661 caattggtcg gctgtcggtc tggcgtagct catttttaga ccggcgatag tagtggctac
      721 tccaagctcg aacaagaaat gggtagtcat gacgcccttg ctaccccaaa actgccagtt
      781 gttcttgatc agctcgcgcc gagttttgcc cggtatgagc agcttctgct tgacgccgac
      841 tcgagtatcc atggtttcgc cgcgcaggat ctctagtttc tcttctagcg gatagtgatg
      901 agccggagtc aagccgtcga ctatagcatg ggctaaccaa gccgcttcga aagctgctcg
      961 ctctagattg tcggcgacca gcgcctcgac caagttatgt aggtgatcat cgatgatatc
     1021 gagaagctgg cctttgttgg ggtttttcgg atcgataaag tgccacaact catccttgcc
     1081 ggggctcttt cgtttgatac cgtcaggacc gttattgcct tcaaagtgca ggatttgttt
     1141 gacagtcggg aaattgactc cggccggcaa gaccggcttg agctggcgac gagccacccg
     1201 gtcaatcttc tgatgggcgc cgataacctg gcctgatttg ttgcgaaaag tagttcctga
     1261 atacatagtt atttgacgtt agatgttaga cgctggacgt ttgatgctgg atgtctcttt
     1321 ctatcgtcta acgtctatct tccatcgtct agtttagcgt tgttaatctt tggtaacggt
     1381 cgacctcccg ggacaaccta tcatattgta gcaagtcaaa atcctgagcg accaaatctc
     1441 gagctgcggc ctgggccttt ttgagcaaac cggtgtcagc caagttagcc accgccaggc
     1501 gaagctgacc gtgctgggct cggccgtaga tctcgcccgg accgcgtagc tccaagtcct
     1561 tctcggctaa gtaaaacccg tcgtttgatt tctccatctc ttttaagcgc tggctcggct
     1621 tagctgaagt cgacgggatc aggtagcaat aactctgggt atccgaccgg ccaacccgac
     1681 cgcgtagttg gtgcaactga gccagaccga aacgatcggc accctcgatg atcatgacac
     1741 tggcattcgg cacatcgacc ccgacttcca ctaccgtagt ggtgaccaat atgtcgatcc
     1801 tgtgatcgat gaaatcctgc ataacttcgg ctttttggtc ggccggtaat ttgccgtgca
     1861 tcaagccgat ccgacggttt ttaaaaatag aattcttcag ccgcttgaac tcagcttcga
     1921 ctgatttttg ctcgccgtct ttgccctcat cgataagtgg gcagactaca taaacctggg
     1981 cgccatcagt aatttgctgg tcgatggtgg cgtagagttg cgcccggcta ttgggtgaag
     2041 tgatcttggt caggatcggt ttgcgtccca ctggtttctg ccgaatcaac gagatatcta
     2101 gctcaccata aaccgtcagt tggagtgaac gcggaatagg cgtagccgtc atagacagta
     2161 catgtggcat cttttcactt ttatcaaaca gggtttgacg ttggcgcaca ccgaagcgat
     2221 gctgctcatc gatgacgacc agaccgagct tatgaaactt gaacttatcg gttagcaagg
     2281 cgtgagtacc gacgacgacg gccgaaccac catcctcgat cgattggtac aggttcgtcc
     2341 tggctttgcc cttgacgttg ccggttagca aaccaacgct
//
LOCUS       DASSBW010000051         7992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1525368, whole genome shotgun sequence.
ACCESSION   DASSBW010000051 DASSBW010000000
VERSION     DASSBW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525368"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFG56_01390"
     CDS             complement(<1..800)
                     /locus_tag="VFG56_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6622568.1"
                     /translation="MKKRELIELARPLYDDDQLAELTEAIDYASTKHRGQKRASGEPF
                     LTHPLSVAASLVDWAMDIDTVIAGVLHDTVEDTDATLTEIENFFGRDVAFLVDGVTKV
                     TEARAGMRNLDGYLPATKDNLTKLMIAVGEDVRVIIIKLADRLHNMQTLKHLPREKQR
                     KIALETIEVFAPLADRLNMGRIRVQLEELSFSYLEPKTYKRLKKLMKDRLGKSHRKLA
                     TVRHDIDKELTGHGIDHQIDGRIKSIYSLHKKLQKVGDIDQIYDLIALR"
     gene            902..1915
                     /gene="gap"
                     /locus_tag="VFG56_01395"
     CDS             902..1915
                     /gene="gap"
                     /locus_tag="VFG56_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET6622569.1"
                     /translation="MKKIAINGFGRIGRNAFKIAFERTDLEIVAINDLTDTRTLAHLL
                     KHDSSYGEYEHQVDFSEDSLTVDGKTIKVLAEKDPANLPWKNLGVDVVIESTGFFVKP
                     ELAKTHIDPAGAKKVVISAPAKGDGADTIVLGVNDEKIKDGTTEVVSNASCTTNCITP
                     VMNVLESHFGVESAMMTTVHSYTASQKLQDAPAKDLREARAAAENIVPTTTGASIAAA
                     KALPALENRFGGMSIRVPSPVVSLSDITLITKKSTTVEEVNNVFKEAAKQPFYQGILD
                     VTEEELVSTDFRGNSHSAIVDLPLTAMVGDKMLKVVAWYDNEWGYSNRLVEVTADIAK
                     TLE"
     gene            1922..2371
                     /locus_tag="VFG56_01400"
     CDS             1922..2371
                     /locus_tag="VFG56_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET6622570.1"
                     /translation="MKIYLGSDHQGFHLKEKLLAYLSKRGVEVEDVGDRELKPDDDFP
                     QFAGMAAIKLLGDDDTARAILICGGGQGMAIAANRFRGIRAVVIWNEHEAKLSRNDNN
                     ANVLCLPSHIVDNDDNAWQAIVDTWLDTPFAGAARYVRRNRQLDELA"
     gene            2404..2847
                     /locus_tag="VFG56_01405"
     CDS             2404..2847
                     /locus_tag="VFG56_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622571.1"
                     /translation="MPKTRSKKKVQPLSLHQLITALSFWGSSVRFLTFLMLAAVSLVA
                     VLSQAGLTVDYVQQTINLFIYVLLSFLLFDVGFVMLGRGLPLKPGVDRLVICVVDLLV
                     VCWYVIPRFALVSTDLANAIVWLILGVLVMLSLRAVLGLLFTTES"
     gene            2844..3473
                     /locus_tag="VFG56_01410"
     CDS             2844..3473
                     /locus_tag="VFG56_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622572.1"
                     /translation="MSLICPTVLASDGASYVAQMERVAGLSSRLQIDLMDGDFAEPRS
                     VNLAQVWWPDGVEADIHLMYRQPASELESLVSLKPNCVIIHAEADGDLAGVIEHLKKF
                     DIKTGLALLKPTQPDQVNDLIGLVNHVLIFSGDLGKFGGQADMAMLDKIKQIRAINPN
                     AEIGWDGGANLSNVQQLAAAGVDVINVGGALQQVDNPAKAYANMVSEIG"
     gene            3482..4345
                     /locus_tag="VFG56_01415"
     CDS             3482..4345
                     /locus_tag="VFG56_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6622573.1"
                     /translation="MNGGQLTIKQMQATANNIRADIIKMLLAAGSGHSAGPLGLADIF
                     TALYYGVMKHDPKNPDWSDRDILVLSNGHCVPVRYATMARAGYFDRDELKTLRQLGSR
                     LQGHPERTRLPGLESTSGPLGSGLSQAAGMAYVLGKIDHNHHRFVYTVVGDGELDEGN
                     NWEAIMFAGKNQLSQLVAIVDRNNIQIDGNTEDIMPLEDLKAKWQAFGWHVQEVDGHN
                     IESVIEACSMGRAVESQPSVIIAHTIPGKGVDFMEYDYHWHGTPPNAQQAKKALHELR
                     TLGGRIRGEHE"
     gene            4338..5330
                     /locus_tag="VFG56_01420"
     CDS             4338..5330
                     /locus_tag="VFG56_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET6622574.1"
                     /translation="MSDLHLTSKIGHDDIGQEPIRAGFGRGLKKAGENNDRIVALCAD
                     LTDSTKMSAFKEAFPERFVEIGVAEQNLVTVAAGMAAMGKVPFTSSYAAFSPGRNWEQ
                     IRTTVCLNDQPVKVVGSHAGVSVGPDGATHQMLEDIALMRVLPNMVVIAPGDSVEAEK
                     ATLAIADNGKPSYLRLAREATPIFTTDKTPFEIGPAYVLRHGDAVSLMATGTMTYQAL
                     VAAKLLADKGLDVEVVHVPTIKPLDVATIVESVKRTGRVVTLEEAQAAGGFGSAIAET
                     LAEHLPCPIKRIGMQDRFGESGQPDQLLEHFGLDAKSIAHSVQQFVNNSYQYHR"
     gene            5339..6250
                     /locus_tag="VFG56_01425"
     CDS             5339..6250
                     /locus_tag="VFG56_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6622575.1"
                     /translation="MAKSIKEIRDSCVRARHLMQRSRRQKFAVGAFNIDNQETLIAVS
                     RAAQAKNSPVLVEVSHGEVEAMGLDNIRDMVDNYRAEYGVEMYLNLDHSPTVEACKAA
                     IDAGFEFIHIDISQADHEASGEEIVAKTKEVVDYARFTGALVESEPHYFGGSSNLHEE
                     EIDYEAIKKTFSTPEGAKRFVEATGIDTFAAAIGNLHGKYPVPKQLDLELLKNLRGAI
                     DCQISLHGGSGTPLEQFEEAARIGVSKININSDMRYAFRKTLEKVLHDNPDEYAVVKL
                     MPEVYASVQVVVEDKIEAFGSADKAVV"
     gene            complement(6247..6831)
                     /locus_tag="VFG56_01430"
     CDS             complement(6247..6831)
                     /locus_tag="VFG56_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622576.1"
                     /translation="MSEAIKRPENIEAGLRLDLDDQYQAAAIISLIRKMAAHGAELHP
                     LDAVLQQQYDEGLTIIDNISLAAKMSCVYAYKGFDDLADYLHRNIPKVTARSHNSLTE
                     FWRRVGLVDDRYKAPNNDLELALGMLTRQDDFFNQVYAVSNLDRLFDIEADITQFITE
                     HHALEEDKDGRWNADYFAVALREYDFWYGHYDAV"
     gene            6897..7868
                     /locus_tag="VFG56_01435"
     CDS             6897..7868
                     /locus_tag="VFG56_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET6622577.1"
                     /translation="MGENQTPTIVAIGKATQDVFLKSREFDPKTEGQVAYTHLPLGAK
                     LDVGEATFSTGGNATNVAVTLARQGLDSHFIWALGDDPASLSILKAMDDEHVDTSLVE
                     QRPEYQASYSTILLSPDGERTILNFHGTLMNYKSLDGSLSQIQSADWLYPSSLGDYEL
                     LDLVLRHAKTLGAKVMLNPAGAELAEPVKLKPLLEDVDILCLNKEEMQGLVHGDSLEE
                     LVRHGLNLCPVVIVSDGPNGVCASDGKTVVTAGMYEDVPVVDRTGAGDAFASGFLSQW
                     AIGKSLKESIVFASANSTSVVGQIGAKSGILSAGVELHDMDIEEVNV"
BASE COUNT         1973 a         1925 c         2085 g         2009 t
ORIGIN      
        1 cgcagggcta ttagatcata aatctgatca atatcgccaa ctttctgcaa ctttttatgt
       61 aacgagtaga tgcttttaat tcgaccatca atctgatgat cgatgccatg tcccgtcagc
      121 tctttatcta tgtcgtggcg gacggtggcg agtttacggt gtgatttacc gagacgatcc
      181 ttcatcagtt ttttgagacg cttgtaggtt ttgggctcca ggtaactgaa gcttaattct
      241 tcaagctgga ctcgtatccg acccatgttg agtcggtcgg ctagtggtgc gaatacctcg
      301 atcgtttcca gtgcaatctt acgttgcttt tctcgaggca ggtgcttcaa ggtctgcata
      361 ttgtgcaatc ggtcggctag tttgatgatg atgacgcgaa catcttcgcc gacagctatc
      421 attagcttgg tcagattatc ttttgttgct ggtaaatatc cgtccaagtt gcgcataccg
      481 gccctggctt ccgtaacttt ggtgactcca tcgaccagaa aggctacgtc gcggccgaag
      541 aagttctcga tttccgtcaa ggtagcatcg gtatcttcga cggtatcatg tagtacaccg
      601 gctatgaccg tatcgatatc catggcccag tcgaccagag atgccgcaac acttaagggg
      661 tgtgtcaaaa aaggctcacc tgaagcccgc ttttggccgc ggtgcttggt cgaagcataa
      721 tcaatcgcct cagtcagttc ggctaattga tcatcgtcgt atagtggcct cgctagttcg
      781 atgagctcgc gcttcttcat gtattaccag tatagacgaa aacagccgcc tgattgttca
      841 tgtcggtttg attggttata atcaaattaa gcttacgcta taaaaccaaa aaggagcatc
      901 aatgaaaaaa atcgccatca atggcttcgg acggatcggc cgtaacgcct ttaagattgc
      961 ttttgaaaga accgatctcg agatcgtggc tatcaacgac ctaactgaca cccgtacgct
     1021 agctcatttg ctcaaacatg actccagcta cggcgaatac gagcaccagg ttgactttag
     1081 tgaagatagc ttgacggtag acggtaagac catcaaggtt ctggccgaaa aagaccccgc
     1141 taacttgcca tggaaaaatc ttggcgtcga tgtggttatc gagtcgaccg gttttttcgt
     1201 caagcctgag ctggccaaga ctcatatcga cccggctggt gccaaaaaag tcgttatcag
     1261 cgctccagcc aaaggagatg gcgctgacac gatcgtcttg ggtgtcaacg acgaaaagat
     1321 caaagacggt actaccgaag tcgtgtctaa cgcctcttgt accaccaact gcatcactcc
     1381 agtcatgaat gtcctagaga gccacttcgg tgtcgagtcg gctatgatga caaccgttca
     1441 tagctatact gcttcacaaa aactacagga tgccccagct aaagatctac gggaagctcg
     1501 agcggcagcc gaaaatatag tgccgactac aaccggggct tcgattgcag cagctaaagc
     1561 tctgccagcg ctcgaaaacc gttttggggg tatgagcatc cgggtaccta gcccggttgt
     1621 cagtctgtca gacattacct tgataaccaa gaagtctact actgtcgaag aagtcaataa
     1681 cgtctttaag gaagcggcca aacaaccctt ttaccaagga attttggatg ttactgaaga
     1741 ggagctggtc tcgactgatt ttcgaggtaa ctcgcattcc gctatcgtcg acttgccgct
     1801 gacagctatg gttggcgaca agatgctcaa ggtcgtggcc tggtatgaca acgaatgggg
     1861 ctactccaac cgcctggttg aagttaccgc cgatatcgct aaaacactcg agtaatccga
     1921 catgaagatt tatctaggtt cagatcacca aggcttccac ttaaaggaaa agctgctagc
     1981 ttatttatcg aagcgcggcg ttgaagtcga agatgtcggc gatcgagaat tgaagcccga
     2041 cgacgacttt ccgcagtttg ccggcatggc agctataaag ttgctcggtg atgatgatac
     2101 ggctagggct atcctgatat gcggtggtgg tcaaggcatg gctattgccg ctaatcgttt
     2161 ccgcggcatt cgggccgttg tcatctggaa tgaacacgaa gccaagctat ctaggaacga
     2221 caacaatgct aacgtactct gcttgccgtc tcatatcgtt gacaatgacg acaacgcttg
     2281 gcaggcgatt gtcgatacct ggctagacac gccatttgcc ggggcggcgc gctacgttag
     2341 acgtaaccgc caacttgatg agcttgcata agcttttttg gcgctttatt atgagtatta
     2401 accatgccga aaactagatc caagaaaaaa gtccaaccgc tgtcgctcca tcagctgatt
     2461 acagccctgt ctttctgggg tagctcggtt cgttttctga catttttgat gttagctgcc
     2521 gtcagtctgg tagcggtatt gagccaagct ggcttaaccg tggactacgt ccagcagacg
     2581 atcaacttgt ttatatatgt actgctcagt tttctgctgt ttgatgtcgg ttttgttatg
     2641 ctgggacgcg gtttaccgct taaaccaggc gttgatcgtc tagtcatatg cgttgtcgat
     2701 ctgttggtgg tttgttggta tgttatcccg cgttttgctc tggtgtcgac cgatctagcc
     2761 aacgccatag tctggttgat cttaggtgtc ttagtcatgc tgtccttgcg ggcggtttta
     2821 ggtttattgt tcacgacaga atcatgagtt tgatttgtcc gacagtccta gctagcgatg
     2881 gggcgagcta cgttgctcaa atggagcgag tcgccggcct tagctctaga ttacagatcg
     2941 atttgatgga cggtgacttt gccgaaccgc gtagtgttaa cctggctcaa gtctggtggc
     3001 ccgacggggt agaggcggat atccatctga tgtaccgcca gccggcgtca gagttggaga
     3061 gcctggttag tctcaagccg aactgtgtca tcatccacgc cgaagctgac ggcgacttag
     3121 ccggtgtgat tgaacatctt aaaaagtttg atatcaaaac cgggctagcc ttacttaagc
     3181 caacccagcc tgatcaagtg aatgatttga taggtttagt caaccacgta ctaatcttta
     3241 gcggcgatct cggtaaattc ggtggccaag ccgatatggc tatgttagac aagatcaagc
     3301 agattagagc catcaatccc aatgctgaga ttggctggga tggtggtgct aacctgagca
     3361 acgtccagca actggctgcg gctggtgtcg atgttatcaa tgtcggcggc gcccttcagc
     3421 aggtcgataa tccagctaaa gcttatgcta atatggttag tgaaatagga taaagaaaaa
     3481 gatgaacggt gggcaactaa caattaagca gatgcaagct acggctaaca atatccgggc
     3541 tgacatcatc aagatgctgc tggcggccgg ttcggggcat tcggctggtc cacttggtct
     3601 agctgacatc ttcaccgcgc tttactacgg cgtgatgaag cacgacccca aaaaccctga
     3661 ttggtctgat cgcgatatcc tggtcttatc caacggccac tgcgtaccgg ttcgctatgc
     3721 caccatggct agggccggtt attttgaccg tgacgagttg aaaacccttc gtcagcttgg
     3781 ttctagattg cagggtcacc cagagcggac taggttgccc ggcctggaaa gcaccagcgg
     3841 tccgttaggc agtggcctca gccaagccgc tggtatggct tatgtgctcg gcaaaattga
     3901 ccataaccat catcggtttg tctacactgt tgttggggat ggcgaactag atgaaggtaa
     3961 caactgggag gcgataatgt ttgccggcaa gaatcaactc agccaactag tggccatcgt
     4021 tgaccgtaac aatatccaaa tcgacggcaa tactgaagat atcatgccgc tcgaggacct
     4081 gaaggctaag tggcaggcct ttggttggca cgttcaggaa gttgacggcc acaatatcga
     4141 atcagtcatc gaagcttgtt cgatgggtcg cgccgtcgaa agtcagccta gtgttatcat
     4201 cgcccatact attcccggta agggcgtgga cttcatggag tacgattatc actggcatgg
     4261 tacgccgcca aatgcccagc aggctaaaaa agctctacat gagctccgaa ccctaggcgg
     4321 acggataaga ggtgaacatg agtgatttgc acctaacttc aaaaattggt catgatgaca
     4381 tcggccaaga gccgattcgg gcaggttttg gtcgcggcct aaaaaaggcc ggcgaaaaca
     4441 atgaccggat cgttgctctg tgcgccgatc tgaccgattc gaccaagatg agtgctttta
     4501 aggaagcttt cccggagcgc tttgtcgaga tcggcgtagc cgaacaaaac ctagtcactg
     4561 tggctgctgg tatggcggct atgggcaagg ttccgttcac tagttcttat gctgcctttt
     4621 caccgggtcg taattgggag caaattcgta ccactgtctg tttgaatgat caaccggtca
     4681 aggttgtagg ctcgcatgcc ggtgtttcgg tcgggccgga cggggctact caccagatgc
     4741 tggaagatat cgccctgatg cgagtgctgc cgaacatggt ggtgatagcg ccgggtgact
     4801 cggtcgaagc cgagaaagct acgctagcta tcgccgacaa cggcaagcca agctatctgc
     4861 gattggctcg cgaagctacg ccgattttta ctactgataa aacaccgttt gaaatcggtc
     4921 cggcttatgt cttaaggcac ggcgatgccg tatcgttgat ggccaccggc accatgactt
     4981 accaagcttt ggttgccgcc aaactgctag ctgacaaagg gttagacgtc gaagttgtgc
     5041 acgtgccgac gatcaagccg ctcgatgtgg ctacgatagt tgagtccgtc aagcggactg
     5101 gccgagtcgt cacgcttgaa gaagctcaag cggctggcgg tttcggatca gccatagccg
     5161 aaacactagc cgagcatctg ccgtgcccga tcaagcgtat cggtatgcag gatcgattcg
     5221 gtgaatccgg tcaacccgat cagctgcttg agcactttgg tttagacgcc aaaagcattg
     5281 cccactcagt ccagcagttc gtcaataatt cttaccagta ccacagatag gagacaatat
     5341 ggccaaatca atcaaagaaa ttcgtgacag ttgcgtgcgc gcccgccact tgatgcagcg
     5401 atctcgccga caaaaatttg cagttggtgc attcaacata gacaaccaag aaacactgat
     5461 agccgtgtcg agagcagccc aagccaagaa ctctccggtt ctagttgagg ttagtcacgg
     5521 cgaagtcgaa gccatgggtc tcgataacat acgcgatatg gtagacaact accgggccga
     5581 gtatggtgtt gagatgtatc tcaatctaga tcacagcccg acggttgaag cctgtaaggc
     5641 tgctatcgat gccggctttg aattcattca tatcgatatc tcccaggctg accacgaggc
     5701 ttccggggaa gagattgtgg ctaagaccaa ggaagtggtt gattacgcca gatttactgg
     5761 cgccttggtc gagagtgaac cgcattactt cggtggaagc tctaatttgc acgaggaaga
     5821 gattgactat gaagcgatca aaaaaacttt ttcgacaccg gaaggcgcca agaggttcgt
     5881 cgaggccacc ggcatcgata cctttgcggc cgctatcggc aacttgcacg gtaaataccc
     5941 ggtgccaaaa cagcttgatc ttgaactact aaagaatctg cgcggcgcca tcgactgcca
     6001 gatcagtctg cacggtggtt ccggtactcc gctggagcag ttcgaagagg ccgcacgtat
     6061 cggcgtctcc aaaatcaata tcaactccga catgcgttat gctttccgta agacgttaga
     6121 aaaagtccta cacgataacc cagacgaata cgcagtcgtc aagctgatgc cagaagtcta
     6181 cgcttctgtg caagtcgtgg ttgaagacaa gattgaagct ttcggctcgg ccgacaaagc
     6241 cgtagtttag acggcatcat aatggccata ccaaaaatcg tattcgcgta gcgccacggc
     6301 aaaatagtcg gcattccagc ggccatcttt gtcttcttcg agcgcatggt gctcggttat
     6361 aaattgcgtt atatcggctt cgatatcaaa caagcgatcc aggttggaaa ccgcatagac
     6421 ttgattaaag aaatcatcct gacgggtcag catgccgaga gctaactcca ggtcgttgtt
     6481 gggagctttg tagcggtcgt cgacgagacc gactctgcgc cagaattcag ttaagctatt
     6541 gtggcttcga gccgtcactt tcggtatatt acgatgtaag taatctgcca ggtcatcaaa
     6601 gcctttatag gcgtaaacgc aactcatttt agccgccagc gatatgttat ctatgatagt
     6661 caggccctcg tcgtattgtt gttgcaaaac agcgtcaagc gggtgcaatt cggcgccgtg
     6721 tgcagccatc ttcctgatca ggctgatgat ggcagccgct tgatactggt cgtctaaatc
     6781 aagccgtagg ccagcttcga tattttcagg tcgttttatg gcttcactca tcaatgcttt
     6841 agcataattt ggcgatttaa ctgacgcaag cacaagtgct atactaggag acaaggatgg
     6901 gcgaaaatca aacacctact atagtagcca taggtaaagc tactcaggac gtattcctaa
     6961 agagtcgtga atttgaccct aaaaccgagg gtcaagtagc ttatactcac ttgcccctcg
     7021 gcgctaaatt agatgtcggc gaggctactt tttcaaccgg tggcaacgct accaatgtcg
     7081 ccgtgacctt ggcgcgccaa gggcttgact cccatttcat ctgggcgctg ggtgacgatc
     7141 cggcttcgct tagtatccta aaagccatgg atgatgagca cgttgatact agcctggtcg
     7201 aacaacgacc cgagtaccag gctagttatt caactatttt attatcgcca gatggtgagc
     7261 ggaccattct taatttccac ggcacactta tgaactataa gtctctcgat ggatcgctca
     7321 gccaaatcca atcagccgac tggctttacc cgtcatcact gggcgactac gagctacttg
     7381 atctggtgct tcgtcatgcc aagacactag gtgccaaagt tatgctgaac ccggctggcg
     7441 ctgagctagc cgaacctgtc aaattaaagc ctctgcttga agatgtcgat atactctgcc
     7501 tcaataagga ggaaatgcag ggcttggttc atggcgacag cctggaagag ttggtgcgtc
     7561 acggccttaa tctttgtccg gtagtgatcg tgtcagatgg cccgaatggt gtttgcgctt
     7621 cggatggcaa gactgtcgta actgctggta tgtatgaaga tgtaccggtg gttgaccgga
     7681 ctggagccgg tgatgccttc gcttctggtt ttttgagcca atgggcgatc ggtaaaagct
     7741 tgaaagaatc aatagtcttc gctagcgcta attcgacttc tgtagtcggt caaattggtg
     7801 ccaagagcgg catcttgtca gctggagtcg agttgcatga tatggacatc gaagaagtaa
     7861 atgtataggg agacatatgt caaagatttt aagagattta ctagacgctg aagaaccggt
     7921 tttctcgcta ggactacgtc agttggagca ggctagtcat cagcccggac aagatgccag
     7981 gctgattggt ga
//
LOCUS       DASSBW010000052        11738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1391179, whole genome shotgun sequence.
ACCESSION   DASSBW010000052 DASSBW010000000
VERSION     DASSBW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391179"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFG56_01440"
     CDS             <1..682
                     /locus_tag="VFG56_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622578.1"
                     /translation="VLVSASGGKHAPIIAKAAKDHGKHVTLLTNTINSPTRQALDEAD
                     LTEYVFPKNREPYTYNTSTYLSMILGKTGEDPAAISRFIEEHVVSLSLPDFSNFDKYY
                     LVVPPEFSGIIRMLEVKFIELFGRNIARDVESSEYVRHATTVAPANELFISFGQSNQT
                     WGKPENRFDVPLPESADYGAMMAVGYYVIAQIQKAQPAYFKDNIVAYCRFISGIFGET
                     ISPIVEGN"
     gene            complement(736..1119)
                     /locus_tag="VFG56_01445"
     CDS             complement(736..1119)
                     /locus_tag="VFG56_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HET6622579.1"
                     /translation="MDSTTKNTSQTLSVLIFLFGIWLIISPYILNYVAAQAIWQQTVA
                     GIIIAILAAVRLAMPRQTWASWVNAVLGAWMIIAPFATGYQTSAEHWNEVVFGILVLI
                     GALWNASLSPSSTVIHRRGGPQAAH"
     gene            1222..1899
                     /locus_tag="VFG56_01450"
     CDS             1222..1899
                     /locus_tag="VFG56_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6622580.1"
                     /translation="MLSSKEMKLKFLGKSHQTGDVWTFSFAVPVGKTWLAGQSIRLEL
                     PVGYDIEEKRFTISSAPAEDRLDITTSLSGSKFKNHLDRIRSGQMIEAQSIEGDFTWS
                     DDSRPKVLVASGIGITPFRAIIKQLEFAGEDLNFSLIYADRRNQLVFEDWLSGLAKHH
                     SAFKLSLIRGRRLQIGDIVLQPNSLVYLSGPSTMVHQLSRQLIDNGVAEQDIKRDSFT
                     GSPGWIG"
     gene            1921..2736
                     /locus_tag="VFG56_01455"
     CDS             1921..2736
                     /locus_tag="VFG56_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET6622581.1"
                     /translation="MRSLNGAELADFIKVRQLKQVRNLRQEHKIVPKLAIVQTVDSSV
                     IDSYAGLKRRYGADILVDVDVHKINQAESMALVDQLNQDEAVHGIIIQLPLADPTQTD
                     ELVNLVAPAKDVDGLGEAADFDPATPIAINWLLTGYNVDLNNKSIAIVGAGRLVGAPL
                     ERMWRQSGLEPLVVDEHTDEHQKQAILTKADIIISAAGVPGLITSELIRPGAVVVDAG
                     TATSGGKLVGDVDPAVRQRHDLTITPEKGGVGPLTVAALFDNLIRAARKTITS"
     gene            complement(2726..3094)
                     /locus_tag="VFG56_01460"
     CDS             complement(2726..3094)
                     /locus_tag="VFG56_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6622582.1"
                     /translation="MSRIAIIEDDPVINQMYRMKFETEGFNVRIADNGQDGIVMVEDF
                     KPDLILLDMQMPGMNGDEALKHIRKLKTAKTTPVIVLTNLGEEEAPKDLRQLGILSYI
                     VKADLTPSQVVERVRTALKK"
     gene            3134..4120
                     /locus_tag="VFG56_01465"
     CDS             3134..4120
                     /locus_tag="VFG56_01465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HET6622583.1"
                     /translation="MPAKQSDFQNDRFSLSITSRAKSAGLVKFDVTVKDRYSKRRYGL
                     EKRMFTAFTNQLQHYLASRPHIDFDHPKNFVRRLVYLPPGIRAWSAYMPGGLATYSVH
                     NPEHKRLAGGKRVDYMTRALFRHSTDALGIRSRAYVLSYLVAQRVSRLKAESISWLSL
                     ASGSGQPVYDTIDDLPNVEFSVTITDHDQASLDFARSVYKYERPHVDKIAFKQLDVLA
                     KGQLEQMINLTKPDLVDAMGLFEYLQPAAASQLIEQIYQGIPTGSELIFTNMSHANPH
                     RDLHKRGLGWPGVIVRPIAEVADIIKNAGIPTGSVQVYSPSDRVYNIYRVVK"
     gene            4117..6291
                     /locus_tag="VFG56_01470"
     CDS             4117..6291
                     /locus_tag="VFG56_01470"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6622584.1"
                     /translation="MTNLALILVVVAIVNFTLGGVVFSRKPRDKAAQTFLGLTGGLGL
                     WGLGISFYLLSGDMDRAAFYSNIYYFAAMLMATSLYLFSVSQSHRQLSSWHYLHWLVP
                     VGWLIGVIISPRLLVNFSINDLSHVAISPVGYAVYSAITITYFVAAIVILARGVANTV
                     DHWRRRQLLLVSVSAVLVGVVAITFNLLLPALGIYYLVWVGPLYSIVFALSAAYAVER
                     YKMFDLRATLAKTLAYLLALIVVVLVYTSVLLAVGLRFFGGVSASRSQDLIYIALAIL
                     LAVTFQPLKNFFNRITSRLFFNLNYSPEQVIQEFGDAMLGSVELGGLAEATFSVLNRT
                     LAPADLALIISDDSAATHFIRVSTSTGDGLSRTVVRHLAQSLSLPRYRRRLIIDSQEL
                     FESRDLTLKSLFDDNAVNLAVKLQTKDRQLGYLLVGYRQNGAGYGSSDMYILRTVGDE
                     LALAIENGLQFKQINRFNRTLKERVDSATSKLRQNNRQLLQLDEAKDEFISMASHQLR
                     TPLTTVKGYLSMLMEGDAGKLTRQQQKLTEEAFNSSQRMVFLINDFLNVSRLQTGRFE
                     LEKRPVDLGEVLTQEIGQLQVMAKSRQMKLDCQADKSLPLMELDETKLRQVMMNFVDN
                     AIYYSSSGSTIAISLKLIDHAVEFKVTDHGIGVPKNEQAGLFGKFYRASNARKQRPDG
                     TGVGLYMAKKVIDAHDGQIIFSSQQGKGSTFGFRLPLINIDL"
     gene            complement(6275..6490)
                     /locus_tag="VFG56_01475"
     CDS             complement(6275..6490)
                     /locus_tag="VFG56_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622585.1"
                     /translation="MSKSKKKRNKAYRGADAKAAPPQVHRYQAIQRNALSQWWFDRRR
                     FLKPVIVVGLIAGFITWLIVSFLTNLY"
     gene            complement(6603..7862)
                     /locus_tag="VFG56_01480"
     CDS             complement(6603..7862)
                     /locus_tag="VFG56_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET6622586.1"
                     /translation="MSLVSQLTKRVGLGISSLILAVSALAAPLSAASIAYATDHVSIE
                     NDVKVANVTAGDTSYSNSVNAKVDQVVKVQLWFHNRENPDSGKDAKNLRAKINIPTAE
                     GQHQTITGSVQGDNTNKASDTATVNLSLDNAHLSYVAGSAKWRYNKGAYDGRKECQTG
                     TKAVPTNDPNNCYDTVKISDKVVTNGTGVNLGDMRPCFAYESTVTVLARVKASEVKVN
                     KYVSSYDADHNPSNNDWKLTNSAKPGDKLDYMIRFENKGNTVLNDVVVGDNLPDYMKI
                     VPGTTYIINGNNPKGVAAGTDHVVSGGIDVGDYNPGAAGYVVFSVQIDPINKFAKCGV
                     YTLKNVGVVRPSGMNEFYNTAWTNVRVECAPGETPKTPEQPQSPEKLPETGATSVLSA
                     LFGAGAIGFGLTSYLRSRRNLSDALNR"
     gene            complement(8236..9519)
                     /locus_tag="VFG56_01485"
     CDS             complement(8236..9519)
                     /locus_tag="VFG56_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622587.1"
                     /translation="MNKLQKLTDNQSEKIAKAERKQKQLKQQLEQQRQTTQTVATVAT
                     RRGPPAGVKAQLRQAGLNPKNFTTKIDWNRADAVARGTNAFSNVNPHTQAKMVSSLRG
                     NPKALHTYMQASGLTRAQALDPNAWIPVQATTNVKYSSGMSYNSNGHVVWGDKFVDPA
                     GAVLWVNTHSTKGVVVRALCLNVAYAPVKTHEQPTHPQPPTVTHQPPKCQSDCNQPPT
                     CKEKPNQPKCQETPPCEAHPNSPECNQPPTCKEKPNQPKCQETPPKCTDSESHDDGVC
                     GTPDSGAEQQPVQDNDPKQTAPTPGYHEGDAEQNAEEQGPPCSGDSCDLDQTQSGTEV
                     SDSKDGGKAPGGGTTTPDDQPDQSSQPADSGQDNPEYDGSSHSTNPDEGTSDPVDTPA
                     SNHDDGGSGSSDSGHSDTGSSGDPLGDVSLPGTSG"
     gene            complement(10116..>11738)
                     /locus_tag="VFG56_01490"
     CDS             complement(10116..>11738)
                     /locus_tag="VFG56_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622588.1"
                     /translation="REQARTKNERVSTDTPEDEVIEPEIVEPGAIEDESEPELEIIDV
                     EAIEDSEEVIGSGETAGTKVLSDQEMWAWDYAAEHLKAGYLYDDSEEVSETRQKLNQA
                     RLKLAEVSVKARRGKLFGFKKKRLGEKLTEAEAAYDEAKQAFLEEEVTEFGRAKQAGQ
                     KEAYLAALSAHEDGALMATEANYFMSKENSKTQKSLEWYKSLSKKERLAYGIGASAVV
                     GVLGGITLGFAAAGAMYGSRVGKGYLNREARRADKIEVTDEEIARSLGDRKFVDDEQK
                     KVIYKSTRKKLQKSKLQASKFEKSTLRQSNQDQHDELLGALNDYKKPTKKTLLSAVKR
                     DTSSRRETEAGAYEAALNDAEAEKRAKRKSLYSSVGFAALGGGIGALIEYGPDIADYT
                     SDHTWGGVRESIDEHTEEFSDKVDDLKDEIVDLQHDVDTNNQHIEALEDQNDHLQDKL
                     DDLKDVISDRSEYLYGEFAGQQVDISIPAGGTVWDQLEAQVNDQYPHASYTERERIVG
                     NLLNSLKDKYPTRNFNIVQAGDHFSFTPNPQA"
BASE COUNT         2626 a         2907 c         3063 g         3142 t
ORIGIN      
        1 ggtattagta tcggcatccg gcggtaagca cgcaccgatt atcgccaaag ctgcgaagga
       61 tcatggcaag catgtcacgc ttcttaccaa tactatcaac tcgccgaccc gtcaggcgct
      121 cgatgaagct gatctgaccg agtatgtttt tcccaagaat cgtgaaccgt acacctacaa
      181 cacctcaact tacttaagca tgatcttagg caagaccggt gaagatccgg ctgcgatctc
      241 gcggttcatc gaagagcatg tcgttagcct gtcgctgccc gatttttcaa attttgataa
      301 atactacttg gtcgtaccgc ctgagttctc tggtatcatc cggatgttag aggttaagtt
      361 tatcgaactg tttggtcgta atatcgctcg tgatgtcgaa tctagcgagt atgtccgcca
      421 tgctaccact gtcgcacctg caaacgagct gttcataagc tttggccagt ccaatcaaac
      481 ttggggtaag ccagagaatc gtttcgacgt gccactaccg gagtcggctg actatggcgc
      541 catgatggct gtcggctatt atgtcatcgc tcagattcaa aaagctcaac cagcatactt
      601 caaagacaat atcgttgctt attgccggtt tatctctggg atctttggcg agacaatttc
      661 accaattgtc gaaggcaatt agaacggacg ctttttataa gcgtccgtct ctgtcacaat
      721 caactctcgg attgattaat gagcggcttg tggaccacca cgacggtgta tgaccgtact
      781 gcttgggctc aggctggcgt tccataaagc accgattagc accaggatac caaagaccac
      841 ttcgttccag tgttcggctg aagtttgata gccagttgca aatggagcga ttatcatcca
      901 agcacctaga acggcgttga cccaactagc ccaagtttgt ctaggcatag ccaagcgaac
      961 tgcggctaag atcgcgatga ttataccggc cactgtctgt tgccaaatgg cttgggccgc
     1021 tacgtagttt aggatatatg gcgatatgat cagccatata ccaaatagga atataagaac
     1081 cgagagtgtt tgtgatgtgt ttttggtggt tgaatccata actagcctcc ctgattatta
     1141 agttatgcat ctttatcgta agtcggcagt tgctcccaag cagtgaaatt ttatacacat
     1201 aagcaagttg aaaagcccga tttgttatca tctaaagaga tgaagctaaa gtttttgggt
     1261 aaatcacacc agactggcga cgtgtggacg ttttcgtttg ccgtaccagt cggcaaaact
     1321 tggctggctg gtcagtcgat acgtttagag ttgcccgtag gttatgacat cgaggagaag
     1381 cgttttacta tcagctcagc cccggctgag gataggctgg atatcaccac tagcctaagt
     1441 gggagtaagt tcaaaaacca tctagataga atccggtcag ggcaaatgat cgaggctcag
     1501 tctatcgagg gtgattttac ctggtcggac gatagccggc cgaaagtttt ggtagctagt
     1561 ggcatcggca tcacgccgtt tcgcgccata atcaagcagt tagagttcgc aggtgaggac
     1621 ctgaatttta gtttaatcta cgccgatcgt cgaaaccaat tggtgtttga agactggctt
     1681 tcagggctgg ctaagcacca ctcggctttt aagctgagct tgatacgggg taggcggtta
     1741 cagataggcg atatagtgtt gcagccaaac agcttggttt atttgtccgg gccgtcaacc
     1801 atggttcatc agctcagccg ccagctaata gacaacggtg tagctgaaca ggatataaag
     1861 cgggatagct ttaccggttc accaggctgg atcggttagc tgatttgcta tgatggttgg
     1921 atgagatcac tgaatggcgc tgagttagct gacttcatca aagtgcgtca gttgaagcag
     1981 gtgcggaacc tgagacaaga acacaagatt gtgcctaagc tggccatcgt tcaaacagtc
     2041 gacagttcgg tcatcgacag ttacgctgga ctaaaacgac gctatggcgc ggatatccta
     2101 gtcgatgtag acgtccataa gatcaatcaa gccgagagca tggctctagt cgatcagctc
     2161 aaccaagatg aagcggtgca tggtattatc atccagctgc cgcttgctga ccccactcag
     2221 acggacgagc tggttaacct agttgcgccg gccaaggatg ttgacggatt gggcgaagcg
     2281 gccgatttcg atccggctac gccgatagct atcaattggc tgctaactgg ttataatgtc
     2341 gatttgaata acaaatctat agctatcgtc ggtgccggca ggctggtcgg tgcacctttg
     2401 gagaggatgt ggcggcaatc cggtcttgag ccgctagtcg tcgacgaaca taccgacgaa
     2461 caccagaagc aggctatctt aaccaaagcc gacattatca ttagtgctgc cggtgtacct
     2521 ggtttgataa caagtgaact gatccgtccg ggtgcggtgg tagtcgatgc cggtacggcg
     2581 acatccggcg gcaaactggt tggtgatgtt gatccggcag ttcgtcagcg ccatgacctg
     2641 accataacac ccgaaaaggg tggcgtcgga ccactaacgg tagcagcgct atttgataat
     2701 cttattcgag ctgctaggaa aactattact tcttgagggc agtccgaacc cgctcgacca
     2761 cttgactagg tgttaggtcg gccttgacga tgtagcttaa gatgccgagt tggcgtaggt
     2821 cttttggcgc ttcttcttcg ccgaggttag tcagtacgat aactggggtg gttttggccg
     2881 tctttagctt gcgaatatgc ttcagggctt cgtcgccgtt catgcctggc atctgcatat
     2941 ctagcaggat gagatcaggc ttgaaatcct caaccataac gataccgtcc tggccgttgt
     3001 cggctattcg aacattgaaa ccttcggttt cgaatttcat acgatacatc tgattgataa
     3061 cagggtcgtc ttcgatgatg gcaattctac tcatatcact tataataact cataaacata
     3121 agcgagtcgt taaatgcctg caaaacaatc cgatttccaa aatgatcgtt ttagcctatc
     3181 gattacctcg cgtgctaaat cggccggcct agttaagttt gatgtgactg tcaaagatcg
     3241 gtatagcaag cggcgttatg gcctggaaaa gcgtatgttt acggctttca ccaatcaact
     3301 gcagcattat ctggccagcc ggccacacat agattttgat catccaaaga acttcgtacg
     3361 ccgattagtt tatttgccgc ccggtattcg ggcttggtca gcctatatgc cgggcggtct
     3421 agctacttat tccgttcata atcccgaaca caagcgtctg gctggcggta aacgtgtcga
     3481 ctatatgacc cgggctttgt tccggcattc tacggatgca ctaggtatac ggtcgcgggc
     3541 ctacgtctta agctaccttg tggctcagcg agtcagccga ttgaaagcag agtctattag
     3601 ttggttgagc ctggcgagtg gcagtggtca gcccgtgtat gatacgatcg acgatttacc
     3661 gaatgtagaa tttagtgtga cgatcaccga tcatgatcaa gcctcacttg attttgcccg
     3721 cagtgtttat aagtatgagc ggccccatgt cgataagatc gccttcaagc agctcgatgt
     3781 tctggctaaa ggccaattag aacaaatgat aaacctgaca aaaccagatt tggttgatgc
     3841 catgggctta tttgagtatc tgcaaccggc tgccgctagt cagttaatcg agcagattta
     3901 tcagggaata ccgactgggt ccgaactgat ctttactaac atgagtcacg ccaatcctca
     3961 ccgcgaccta cataagcgag gcctaggttg gccaggcgta atcgtcaggc cgatcgctga
     4021 agtagccgac attatcaaaa acgccgggat accgaccggt tcagtccagg tttatagtcc
     4081 gagcgatcgg gtttacaaca tctatcgggt ggtcaaatga cgaacctggc gcttattcta
     4141 gtcgtagttg ccattgtcaa cttcacgcta ggtggcgtgg tattttcgcg taagccacgc
     4201 gataaagcag ctcagacatt tctgggccta acaggtggct tggggttgtg gggtctgggc
     4261 atcagctttt atctactgtc tggcgacatg gatcgcgccg ctttctatag caatatctac
     4321 tacttcgctg ccatgctgat ggcaacatcg ctgtacttgt ttagcgtatc gcaaagtcat
     4381 cgtcaattgt ccagttggca ttacctccac tggctagtgc cggtaggctg gctgatcgga
     4441 gtgattatat cgccacggct actagtgaac ttctcgatca atgacctaag ccatgttgca
     4501 atctcgccgg tcggctatgc ggtttatagc gccatcacga ttacttattt tgtggcggct
     4561 attgttatcc tggctcgcgg tgtagccaat actgtcgacc actggcgccg ccgtcagctc
     4621 ctgttagtta gtgtcagtgc cgtcctggtg ggtgtcgtgg cgattacttt caatcttctc
     4681 ttaccggcac taggcatcta ttatctggtc tgggttggtc cactttactc catcgttttt
     4741 gctctcagcg ctgcctacgc cgtagaacgc tataagatgt tcgaccttcg ggctactttg
     4801 gctaaaaccc tggcttatct gctggctttg atcgtagtgg ttctcgtcta cacatcggtg
     4861 cttttggcgg tagggttgag gttcttcggt ggagtgtcgg ctagtcggtc ccaagatctg
     4921 atatatatag ccctagcaat tcttctagcc gtgacttttc agccacttaa gaactttttc
     4981 aaccgcatca ctagccggtt gttttttaac ctcaactaca gtcccgagca ggtcatacag
     5041 gagtttggtg atgctatgct tggctcagtt gaacttggcg gcctagccga ggcgaccttc
     5101 tcggtgctca atcgtactct ggctccagct gatctagctt taataattag cgacgacagt
     5161 gcagcgacgc actttattcg tgtttctaca tctacgggcg atggcttaag tcgaacggtc
     5221 gtcaggcatt tagctcaaag cctgagcttg ccacgctata ggcgacgact gattattgat
     5281 tcgcaggaac tatttgaaag tcgcgacctg acacttaaaa gtttatttga tgataacgct
     5341 gtcaacctgg cagtcaaact ccagaccaaa gatagacagc tcggatactt gttggtcggt
     5401 tatcggcaaa acggtgccgg ttacggttca tctgatatgt atatattgag gacggtaggc
     5461 gatgagttgg ctttggcgat agagaatggt ctgcagttca aacaaatcaa tcgatttaac
     5521 cgtacattga aagaaagagt tgattcggct actagtaagc tacgtcaaaa caaccgtcaa
     5581 ttacttcaac ttgatgaagc caaggatgag tttatctcaa tggcttcgca tcaacttagg
     5641 acaccgctaa cgacggtaaa gggttatctg agcatgctga tggaggggga cgccggtaag
     5701 ctgactcgtc agcagcagaa gctgaccgaa gaagccttca atagttctca acggatggtc
     5761 tttttgatca atgatttttt gaatgtatct aggctacaaa ccggccggtt cgagctggaa
     5821 aagcggccgg tagacttggg agaggtcctg acccaagaga ttggtcagtt gcaggttatg
     5881 gccaagtcac gccaaatgaa acttgattgc caggctgata aatcgttgcc gctgatggaa
     5941 ctagatgaga ctaaactacg ccaggtcatg atgaactttg tcgataacgc catttactac
     6001 tcatcgtctg gctcgacgat tgccatcagt ctcaaattga ttgatcatgc cgttgagttc
     6061 aaggtaaccg atcatggtat aggcgtgcca aagaacgaac aggctggtct gttcggtaaa
     6121 ttttatcgag cttctaacgc tcgaaaacaa cggccggatg gcaccggagt cggcctatat
     6181 atggccaaga aggtgatcga tgcccatgat ggacagatta ttttttctag tcagcagggc
     6241 aagggtagta cgtttggatt tcgtttgcca ctaatcaata tagatttgta agaaaagata
     6301 cgatcaacca agttataaaa ccggcaatca ggccgacaac tatgaccggt ttcaaaaaac
     6361 gtcggcgatc aaaccaccac tgacttaggg cgttgcgctg aatggcttga tagcgatgaa
     6421 cctggggcgg tgctgctttg gcatcagcgc cgcgataagc tttgttgcgc ttctttttag
     6481 acttcgacat aagcaaatta taccaaaaac acccctacta agtaggggtg tcgatgggtt
     6541 ggcacaagcc gcctttatct tgaaggaact tttgtttagc ttaagataaa agaacagtca
     6601 gtttagcggt ttagcgcgtc gcttaagttg cgacggctac gcaggtagct agtcaagccg
     6661 aagccgatgg caccagcacc gaacagggcg ctaagcacgc tagtggcacc ggtttccgga
     6721 agtttctccg gagactgcgg ttgctcaggt gtcttaggtg tttcacccgg agcgcattca
     6781 acacggacgt tggtccaagc ggtgttgtag aactcgttca taccgctggg tcgaactaca
     6841 ccgacgttct tgagggtgta tacgccacac ttggcgaatt tgttaatcgg gtcgatttga
     6901 accgagaaaa caacataacc agcggcgcct gggttgtagt caccgacatc gataccacca
     6961 ctaacaacgt ggtcagtacc ggcagcaaca cccttagggt tgttaccgtt gatgatgtag
     7021 gtggtacccg ggacaatctt catgtagtcc ggtaggttgt cgccgacaac gacgtcgttc
     7081 aagacggtgt tgcctttgtt ctcgaaccgg atcatgtagt caagcttgtc gcccggctta
     7141 gccgagttgg tcagcttcca gtcgttgttt gacgggttgt gatcggcgtc gtatgaggaa
     7201 acatacttgt tgaccttgac ttcacttgct ttgactcgtg ctagaacggt aactgttgac
     7261 tcgtaagcga agcatggacg catgtcgcct aggttaacgc cggtaccgtt ggtaacgact
     7321 ttgtcgctaa tcttgacggt gtcgtaacag ttgttagggt cgttggtcgg gacagctttg
     7381 gtgccggtct ggcactcttt acgtccgtcg taagcaccct tgttgtagcg ccacttagcc
     7441 gagccggcga cgtagctcag gtgtgcattg tctaggctca ggttaaccgt agcggtatca
     7501 cttgctttgt tggtgttgtc accttggaca gaaccagtga tagtctggtg ctggccctca
     7561 gcggtaggga tgttgatctt tgcccgtagg ttcttggcgt ctttgccaga atccgggttt
     7621 tcgcggttgt ggaaccacaa ctgcactttg acgacctggt caactttagc gttgaccgag
     7681 ttcgaatagc tagtgtcgcc tgcggtaacg ttggcgactt tgacgtcgtt ttcaatcgat
     7741 acgtgatcag tcgcgtaagc gatagaagcg gcggatagcg gggcggctag ggcagaaaca
     7801 gctaggatta agctggatat gcctagaccg acgcgcttgg ttagttgtga aactaaactc
     7861 ataactcgtc tccttaaaat gatttgataa ttaatgttaa ttggctaact tgtgtcgttt
     7921 aggttgttaa ggcgcatctg gcaagcctgt ttaaaagtaa gccacgtata caaacacaaa
     7981 aagtggttct cgagcacgag aatccttaga atcgggacgc ttgactgccg aaaagctttc
     8041 gctaatcagc caaaacatgt ccgcgcgatt catagattct cagtgctcgc taaatcgtga
     8101 taccaacaat tcgtacgtct ccaccttggc caaccccacc taggacacgg ggctcttcga
     8161 aggagatgaa aacgtataaa cgctggcacc tcctaagagg ccatggtcct cttagggctt
     8221 ggctaactgg atgcgtcagc cggaagtacc gggcagactt acgtcgccca gcgggtcacc
     8281 cgagctaccg gtgtcgctgt gaccggagtc actgctaccg ctaccgccat cgtcgtggtt
     8341 cgacgcgggg gtatcgaccg ggtcagacgt tccttcgtcg gggttcgtcg agtgagatga
     8401 accgtcgtac tcagggttgt cttggccaga atcggccggt tgtgacgact ggtcaggctg
     8461 gtcgtcggga gtggtggtgc caccgcccgg agccttgccg ccgtccttgc tgtcagaaac
     8521 ctcggtgcca gactgggtct ggtcgaggtc acatgaatct ccggaacaag gcggaccctg
     8581 ctcctcggcg ttctgctcgg catcaccttc gtggtaaccg ggcgtgggtg cagtctgctt
     8641 cgggtcgttg tcctggacag gctgctgctc ggcaccggag tccggcgtgc cacagactcc
     8701 atcgtcgtga gactcggagt cggtgcactt cggaggcgtc tcctggcact tcggctggtt
     8761 cggcttctcc ttgcacgtcg gaggctggtt gcactccggc gagttcggat gggcctcaca
     8821 cggaggcgtc tcctggcact tcggctggtt cggcttctcc ttgcacgtcg gcggctggtt
     8881 gcagtcggac tggcacttcg gcggctgatg agtcaccgtc ggcggctgtg ggtgagtcgg
     8941 ctgctcatgc gtctttacag gcgcgtaagc aacgttcaga cacagtgcgc ggacgacaac
     9001 gcccttggtc gagtgggtgt tcacccacag gaccgcacca gccggatcga caaacttgtc
     9061 gccccaaacg acgtgtccat tcgagttgta ggacatgccg ctcgagtact tcacgtttgt
     9121 cgtagcctgt accggaatcc aagcgttcgg atccagagcc tgagccctgg taagtcccga
     9181 cgcctgcatg taggtgtgca gagccttcgg gttaccccta aggctactga ccatcttggc
     9241 ttgggtgtgc gggttgacgt tcgaaaacgc gttcgtgccc cttgccacgg cgtcggcccg
     9301 gttccagtcg atcttcgtag tgaagttctt cgggttcaga ccagcctgcc tcagctgcgc
     9361 cttgacgccg gccggagggc cgcggcgggt tgccacggtt gccaccgtct gggtcgtctg
     9421 gcgctgttgc tcaagctgct gcttgagctg cttctgcttc cgttcagcct tcgcgatctt
     9481 ctcgctctgg ttgtccgtca gcttctgcag cttgttcacc tgctgcttga gctgcttcag
     9541 ctcctgcctc tcctcgcggg tctgctgggc gaccgtccgc tggacgttct cctggttgac
     9601 caatcggagt gggctggcaa cgaggaccag cccggcgatc actgcgacga tcagaccgcc
     9661 gacagccgct gcacccagca tcttgccgct gggcgagtgt gacttgacct tggtcttgac
     9721 ctcctggcgc ttggcgcttc gagtctgctt gttgcggttc ttgagttccg ccttcagctc
     9781 gctcttcggc acctggctga gattgttgtc gctcatcgct tttcacctct tcctctagta
     9841 gacgtgtgat tgcgatgcaa cttccgtttg gtaggaagca gccttactga ctaaccctaa
     9901 tcagcaaaac tgcttcctac ctgaaatgaa tcattgatat cttacgaaca cccagtcatt
     9961 acttgacacg gagttatgtt cgtatatgtg acttacttgt taaggcgcgc ttgaagctgc
    10021 agacccacct ctgtacaacc agatgtaaga ataataccac aaacgcttta aaaagtcaat
    10081 gcttttacaa tattcaaaac agataatcaa atagcttacg cttgagggtt tggcgtaaag
    10141 ctaaagtgat cgccggcttg cacgatattg aagttacgag tagggtactt gtccttcaag
    10201 ctatttaaca ggttaccgac tattcgttca cgctctgtgt aactggcgtg cgggtactga
    10261 tcattaacct gagcctcgag ctgatcccag accgtaccgc ccgcagggat agatatgtca
    10321 acctgttggc cagcgaactc accgtatagg tattcgctcc tgtcgctgat gacatccttt
    10381 aagtcgtcca gcttatcttg taagtgatcg ttttgatcct cgagggcttc gatatgctgg
    10441 ttgttcgtgt cgacatcgtg ctgtaagtca acgatttcat ccttcaaatc gtcgacctta
    10501 tcgctaaatt cttcagtatg ctcatcgatg ctttcacgta ctccacccca agtgtgatcg
    10561 gacgtatagt cggcgatgtc ggggccgtat tcgatcaaag caccgatacc gccacctaga
    10621 gcagcaaagc ccaccgagct atagagtgat ttgcgcttag ctcgtttttc agcctcggca
    10681 tcatttaggg cagcttcgta ggcaccggct tcggtctcgc gtcggctaga agtgtcacgc
    10741 tttacggctg agagcaacgt cttcttagtc ggctttttat agtcatttaa ggcgcctagt
    10801 agttcgtcgt gctggtcttg gttagattgt cgcagagtgg acttttcgaa tttggaagct
    10861 tgtagcttac ttttttgcag ctttttgcga gtagatttgt agataacttt tttctgctca
    10921 tcgtcgacga atttacggtc acctagacta cgggcgatct cttcatcggt aacttcgatt
    10981 ttgtcggcgc gacgggcttc tcggtttagg tatcctttac caacccgact gccatacata
    11041 gcaccggctg cggcgaaacc aagtgtgata ccgccaagta ctccgactac ggcactggca
    11101 ccgataccat aagccaaacg ttctttcttc gatagactct tgtaccactc caaactcttt
    11161 tgtgtcttgg agttttcctt gctcatgaaa taattggctt cggtagccat cagagcacca
    11221 tcttcatggg ccgatagggc ggcaaggtaa gcttcttttt gaccggcctg tttggcgcgt
    11281 ccaaattcgg taacttcttc ttctaagaag gcctgcttag cttcgtcgta ggcagcttcg
    11341 gcctcggtca gtttttcgcc caaacgcttt tttttaaaac caaataattt gccgcgacgg
    11401 gctttgacgc taacctcggc taatttcagt cgagcttggt tcagtttctg gcgagtttca
    11461 gatacctctt cggagtcatc ataaagataa cctgctttaa ggtgttcggc ggcgtaatcc
    11521 caagcccaca tttcttgatc gcttaagacc ttggtaccag cggtctctcc ggaccctatg
    11581 acctcttcgc tgtcttcgat tgcctcgaca tcgatgatct ccaactctgg ctccgattcg
    11641 tcttcaatag cacccggctc aacgatctcc ggctcgatta cttcgtcctc gggtgtatca
    11701 gtactaaccc gctcgttttt agtacgagct tgttcgcg
//
LOCUS       DASSBW010000053        11263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_92161, whole genome shotgun sequence.
ACCESSION   DASSBW010000053 DASSBW010000000
VERSION     DASSBW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92161"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFG56_01495"
     CDS             <1..1109
                     /locus_tag="VFG56_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET6622589.1"
                     /translation="GNDLEITPPAKLKLDQMDVKAARRTRSVIMFLGPLLHGYNEFKL
                     PYAGGCNLGSRTVEPHLKGLENFGLDVAATKGFYDASVKSKSPERPIILAERGDTVTE
                     NVLMAAARTQSTTVIRNASPNYMVQDLCFFLQKLGVKIDGIGTTTLTVTGVKDINQSV
                     EYSPSEDPIEAMSFIAAAVATKSEITIKRAPIEFLEIELETLREMGLDFTLSEEYKAK
                     NGQTRLVDITTKPSDLKAPKDKIHPMPFPGLNIDNLPFFAVIAATARGRTLIHDWTYE
                     NRAIYFTELNKLNANIQLLDPHRVYIEGPTRWKPAEIIAPPALRPAVVILIAMLAAPG
                     TSTLRNVYSINRGYEDLANRLNTLGSQIETLREL"
     gene            complement(1096..1722)
                     /locus_tag="VFG56_01500"
     CDS             complement(1096..1722)
                     /locus_tag="VFG56_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET6622590.1"
                     /translation="MRYTHKDGVKQSKKSTPKPLLWLLGISLVAGIYAVSLLAWPALP
                     ISSAEVQSVTDMVKNNQPDPSRNRLYIPKLGLMVDIGRSNGSLTVVKASGNPIDGGNY
                     ILSGNKLSFGPTPESTAERSPLYHVEELESGDDIFIDYQGKRYNYKIDDKQVVSSSDE
                     SFETPTTDPTLTLYTRSDGVLERTRQVIFAHPVGTIEWVNGKAGLKAL"
     gene            1850..1926
                     /locus_tag="VFG56_01505"
     tRNA            1850..1926
                     /locus_tag="VFG56_01505"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1884..1886,aa:His,seq:gtg)
     gene            complement(2118..2194)
                     /locus_tag="VFG56_01510"
     tRNA            complement(2118..2194)
                     /locus_tag="VFG56_01510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2158..2160),aa:Arg,seq:tct)
     gene            complement(2223..2762)
                     /locus_tag="VFG56_01515"
     CDS             complement(2223..2762)
                     /locus_tag="VFG56_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET6622591.1"
                     /translation="MKYVALLRGINVGGNNKISMFELKACFEKLGFGDVKTYINSGNI
                     IFSTSKSDETRLVRSIENCIKDKFNLDIPVVIRSNQQIAEVVKAIPKEWVNNQAMRTD
                     VLFLWTEVDGPEVLEEIKTNPEVDNLIYTAGAIIWNFDRQNYRQSKLHGFIGTHVYKL
                     MTARNINTVRKLAGLMTGA"
     gene            complement(2923..4107)
                     /locus_tag="VFG56_01520"
     CDS             complement(2923..4107)
                     /locus_tag="VFG56_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6622592.1"
                     /translation="MTKTTSQKHQKYVALIAATGLLLINFWAWSLISPLAMTYAKEFS
                     LSPFMLSLLVAAPVMIGSLGRIPLGLLSDRYGGRKLFTVMCFLSAAVVFWLPLVDSEN
                     QLFLAAVLLGIAGASFAAGVPFVNAWFPRNQRGLALGIYALGNGGTAISGLLTPYAAE
                     VLGRTHFFWLIAVLLTLAAVAIGLFGRESSSWRSAKGSSWHRLKQALTWKLTWRLAIL
                     YVISFGAFVALGLYLPVLLSQSYGLESKDAAMRAAGFIILATLARPLGGWLSDRLSGL
                     VVVRACFFVLGLLAILAAVRPDLAPIGTLVYLSMAAVLGLSCGAIFAVIGHRCPPATV
                     GVTTGVIGAAGGLGGFFPPLIMGLSFELFHSYTLALVLLAVVCLVIAFSLRKLFGYTA
                     SY"
     gene            complement(4181..4393)
                     /locus_tag="VFG56_01525"
     CDS             complement(4181..4393)
                     /locus_tag="VFG56_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622593.1"
                     /translation="MNNGDRRIAKEQDVNYEGRPIHRPPELISEPVKGYWTYIWIVVI
                     VVVILAWAFLFGYFSNHQAPYAAPLR"
     gene            complement(4396..5868)
                     /locus_tag="VFG56_01530"
     CDS             complement(4396..5868)
                     /locus_tag="VFG56_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6622594.1"
                     /translation="MDDSMPLVIVITHFVNLIVLLLLIRSGLHILADHPFLYWTDDTR
                     RDNYWLKFGKKEIPEGHVYQAHDEAEKIGHWALPGGEHHDFGNARNWHFVAAIIWLAT
                     GAVYWGYMFATGAWRRLIPTSWSVFPDAWHDLLNYLSFHRPPVEAFQPYDPLQQLTYA
                     AVAFLLPVLMILTAAAMSPALMNRFPKYLLLFGNRRQAARSLHFIGMVLFSVFIIVHV
                     IMVALVYFYRNVQIITFGHTGVNFAAALTLFMAGLLFVLVFHIVITYFTVNHRVRTRQ
                     MLVGILNPVIHTVFGWMTPRKTYKKEDISSFFRINGLPPDTEEFYNLRDNGFKDWRLK
                     VEGLVENELSLSLDELKQMPKQEQITKHICIQGWSAIGEWGGVHMREILERAKPSSEA
                     KYVVFHAYDLYPGGKPFYEALRLSDMNDKYTILAYEMNWKSLPLEHGAPLRLRVERKL
                     GYKMVKYIRSIEFVKDYKHLYGGRGGYREDVILFDNEASI"
     gene            5919..6257
                     /locus_tag="VFG56_01535"
     CDS             5919..6257
                     /locus_tag="VFG56_01535"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6622595.1"
                     /translation="MRYVVFDDFFAVLGNPQRVRILQYLNRHGSKNVSQLCEKLGLEQ
                     SAVSHSLRRLSRCHFVECETRGKERIYSINKDTVQPLFNLIDEHVRKYCVHGCEHWNI
                     KTKEVIHASR"
     gene            6244..6960
                     /locus_tag="VFG56_01540"
     CDS             6244..6960
                     /locus_tag="VFG56_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6622596.1"
                     /translation="MPVVNVKLLKKEQVANGTMAFHWQKPEGFEFRAGQFCDITLIDP
                     PETDSEGDVRGFSLAYAPYENDIVTATRMRDTAFKRVLKDLQEGTEVKFDAPYGDFTL
                     HKTESTPAVFIIGGIGITPVRSMIAQATHDETSHKITLLFSNKTPADAPFISDLRGFA
                     EANPNFTFVPVYTDAAEAEWDGERGYVDAEILKRHVADLSAPIYYLAGPAGMVKAMRQ
                     ILVEAGANEDNIRTEEFSGY"
     gene            complement(7007..7081)
                     /locus_tag="VFG56_01545"
     tRNA            complement(7007..7081)
                     /locus_tag="VFG56_01545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7046..7048),aa:Gly,seq:tcc)
     gene            complement(7199..9568)
                     /locus_tag="VFG56_01550"
     CDS             complement(7199..9568)
                     /locus_tag="VFG56_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6622597.1"
                     /translation="MFDYHSRRAVEARFGAQLRPSIIVSLRVAAIVLILCGLLLLGFA
                     EPIGWLALTLAAWPYCTLVWQRRHLSRLPVEIGDRLDQQLEASVLAALPRDLSAKALA
                     TATMQSNGGRFMAVRLGLAPNFLTDLSSEASQSVEAIWQKARQLAGKGQVSGAHLVAA
                     LVSVQNGFKNILPHLQLDEADVANGAGWYDHLDRLILDHQKPKRTGGLARDWTFGYTP
                     LLERFAANVSRQVEGGSLLSVDLEAHKLALERLQQTFVGGGRQNIALVGPLGVGKTSL
                     VYAFAEKLMNPSVDLPANLRFHQVVNLDAGSLIGSAKGRGDLEELVNALLIEAYRAKN
                     IIVCLDGAELFFEDGVGSVDLSNVLLPVIEGGNLRMILTMEEQRWLKIAARNPALANA
                     LNRVGVEPTNRDETMLVMQDQLISYEFKHKVTYMYQALQEAYRLSERYMYDQAQPGKS
                     LKLLESAADYAESGLVTAGSVQRAVEQGQGVKIGATSDAGEKQKLLQLEDLIHERMIN
                     QTRAVGVVSDALRRARAGVRNENKPIGTFLFLGPTGVGKTELARSLAAVYFGGEDRLI
                     RLDMNEFVAPDDVKRLIADGAADEHSLTAQVMKQPFSVILLDELEKAHDAVLTTLLQV
                     LDEGVLRDIKGREVSFRDAIFIATSNAGADQIRHHIDSGEKLEDFEDLLTDELISSKQ
                     FRPEFLNRFDEIVLFRPLDKKELLQVIDLIITGINKTMAHQKVRLTVDDEAKTKLVDL
                     GYDPRLGARPLRRVVQRTVESVMAKKLLAGEAQAGDTIVITVDDIKSDK"
     gene            complement(9561..9950)
                     /locus_tag="VFG56_01555"
     CDS             complement(9561..9950)
                     /locus_tag="VFG56_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622598.1"
                     /translation="MLLVGFFSWWYGLGWRDQLNLFGERLARTADQWSIGSLSSSLFS
                     PFRQLDTVKVGGPLGVRLRAWLDRLISRFIGVMVRSIMILVGLAVFVGTAFAGLTWLV
                     IWPILPWVPVIGVILTASGWLVWSPYV"
     gene            10000..>11263
                     /locus_tag="VFG56_01560"
     CDS             10000..>11263
                     /locus_tag="VFG56_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HET6622599.1"
                     /translation="MDLSHKLALTAGRLVKKTARLRGGGSALPGLVIEKLDPGFLKRA
                     LADLPRGVAVVSGTNGKTTTTKMVVQLLRDQGLKVFTNPTGSNFTRGVAAALIDQVNP
                     ETGKLDADIAVLELDEAHAVHFIREVKPSYSLILNILRDQLDRFGEIDHTAKLLARVV
                     ESTQSGVVLNREDPLVLKLADNVPDSVKLQYFGLNPDLKDRFPSDQTMRGSYQTDTPD
                     KLPPLHRKDTILDRLDGRQAVFAFGSKNQSPVELKLDGLYNVLNAAAALGLVRLILGD
                     KADETKLMSELAEVKPAFGRGEAVELSDGKLEIVLVKNPSGFRLGLKSYSDSPAATMI
                     CINDNYADGRDMSWLWDVDFDQFANDNKVGVTTVSGTRAYDMALRLQYDGVLVGHVDT
                     DLGKALKQFLTDTTGQRRIYTTYTAMLEL"
BASE COUNT         3010 a         2961 c         2615 g         2677 t
ORIGIN      
        1 acggcaacga cctggagatc actcctcccg ctaagctgaa gttggatcag atggacgtta
       61 aagccgctag gcgtacccgc tcggtcatca tgttcttagg tccgctactt catggctaca
      121 acgagttcaa attaccttat gccggtggct gcaaccttgg atcgcgcaca gtcgaaccac
      181 acctcaaagg cctggaaaac tttggcctag atgtcgctgc cacaaaaggc ttttacgatg
      241 ccagcgtcaa atctaaatca cccgagcgac ctatcatctt agctgagcgc ggtgacacgg
      301 taactgaaaa tgttctcatg gctgccgcta gaacacagtc aaccacagtg attcgaaacg
      361 ccagtccgaa ttatatggtg caagacctat gcttctttct ccagaagcta ggtgtcaaaa
      421 tcgatggtat cggtaccact accctgaccg taactggagt caaagacatc aaccagagcg
      481 tcgaatatag cccaagcgaa gacccgatcg aggcgatgag cttcatcgct gcagccgtcg
      541 ctaccaagtc ggaaataacc atcaaacgtg caccgataga gttcttggag atcgaattag
      601 aaacccttcg tgaaatgggc ctggatttca cactgtccga ggagtacaaa gccaaaaatg
      661 gtcaaacccg cttggttgac atcaccacta agccatcgga cttaaaggcc cctaaagaca
      721 aaatccaccc catgcctttc ccgggtctca atatagataa cctacccttc tttgccgtca
      781 ttgccgctac ggctcgtggt cgcacattga tccacgactg gacttatgag aaccgggcga
      841 tttattttac tgagctcaat aagctcaacg ccaacataca gctactcgac ccgcaccgcg
      901 tctacatcga aggcccaacc cgctggaaac cggctgagat tatcgctcca ccagccctgc
      961 ggccggccgt agttattctg atcgccatgc tagccgcacc cggcacatca acgcttagga
     1021 atgtatactc gatcaaccgc ggctacgaag acttggctaa ccgcctcaac acccttggct
     1081 cccagatcga aacgcttaga gagctttaag cccagccttg ccgttgaccc attcgatagt
     1141 accgaccgga tgggcaaata tgacttgtcg agttcgttct aaaactccat cggaacgcgt
     1201 atagagagtc agcgtagggt cggtggtcgg ggtttcaaat gattcatcgc tcgatgaaac
     1261 cacctgttta tcatctatct tgtagttata acgtttgccc tggtagtcga taaatatgtc
     1321 gtcgcctgat tctagctcct cgacatgata tagcggggag cgttcagcag tagactcggg
     1381 agtcggacca aacgacaatt tattgccgct taagatatag ttaccaccat cgatcggatt
     1441 accactagcc ttgaccacag tcaagcttcc gttcgaccga cctatgtcga ccatcaagcc
     1501 aagtttcggt atgtacagac gattgcgcga cggatccggt tggttatttt tgaccatatc
     1561 tgtgaccgac tgtacttcgg ctgaactgat tggcaaggcc ggccatgcca gcaagctaac
     1621 tgcatagatt ccagcaacca agctgatacc aagcagccac aataatggtt ttggcgtgct
     1681 ctttttagac tgtttgacac cgtctttgtg tgtatacctc atttgtttca attataacaa
     1741 aagctttatg tcttagccga cttttgctcc aaataccact tacttcccct atctgttaaa
     1801 aactgttaaa atagctctgc ttatcaccac agataaatag caagatacgg cgaatgtagc
     1861 tcagttggtt agagcgtcgg gttgtggttc cgaaggtcgt gggttcaatt cccatcattc
     1921 gccccatacg aaaatagtca gtctttctgg gctgactatt ttcgtatggt acccgggtag
     1981 ggtcggcttc gcctcttccg ttcgtcgatg ctcgaagcaa gcttctgcgc gtcgactcac
     2041 ttcaccgaac cttacggttc gagctcgcta gcgcgagcta accatacaaa aaccagccgc
     2101 aagggctgga ttttgtatgg taccccgggt agggctcgaa cctacggcct gaagcttaga
     2161 agtctcctgc tctatccaac tgagctaccg gggcgcgtcg gagttattat aacatctgtt
     2221 aactaagctc cggtcatgag cccagccagc ttgcgaacgg tgttgatgtt acgggcggtc
     2281 atcaacttgt agacatgtgt accgataaac ccgtgtagtt tgctctggcg gtaattctgc
     2341 cggtcgaagt tccagatgat agcaccagcg gtatagataa ggttatcgac ctccggattg
     2401 gtcttaatct cttcaagcac ttccggtcca tcgacttccg tccacaaaaa cagcacatcg
     2461 gtccgcatag cctgattgtt aacccattcc ttagggatag ctttaacaac ttcggcaatt
     2521 tgttgattag aacgaatcac cactgggata tcgaggttaa atttatcttt gatacagttt
     2581 tcgatgcttc ggaccaaccg cgtctcatcg ctcttggaag tagagaaaat aatgttaccg
     2641 ctattgatat aagttttgac atcaccgaag cctagttttt caaaacaagc tttcagctcg
     2701 aacatgctga ttttattgtt gccgccgaca ttgataccgc gtagcaaggc tacatatttc
     2761 atcttctaaa ccttcctctt ggtaccagtt tatatgtatt gattagctaa acaagctaat
     2821 caataccgtg ccagccacca ttaccaaagc gccgatcaac cgctgaccga gctgcctctc
     2881 acgaaatagc caagcaccaa agattaccgt aaaaatcaac tactaataac tagccgtgta
     2941 gccaaatagc ttacgtagac taaaagcaat caccagacaa acgaccgcaa ggaggactaa
     3001 cgctagcgta tacgagtgaa ataattcaaa gcttagaccc atgattagcg gcgggaagaa
     3061 gccacccaaa ccaccagccg cgccaataac accagtagta acaccgacag tagccggtgg
     3121 acaacggtgg ccaattactg cgaaaatcgc gccgcaacta aggcctagta ctgccgccat
     3181 actgagataa accagcgtac cgattggcgc gagatctggt cgaaccgccg ccagtatagc
     3241 cagtaggccc aagacgaaga agcaagccct gaccacaact aagccactca gtcgatcgct
     3301 taaccaacca ccaagtggcc gagctagcgt agctaagatg atgaagccag ctgctcgcat
     3361 ggcggcatct ttcgattcca ggccgtatga ttgactgaga agcaccggta ggtacaggcc
     3421 aagtgctaca aaagcaccaa atgagatcac atagagaata gctaatcgcc aggttaactt
     3481 ccaggtcaga gcttgcttca gccgatgcca gctactacct ttggctgacc gccaactact
     3541 gctttcacgg ccaaacaatc ctatggctac ggcagccagc gttagcaaaa cggctatcag
     3601 ccaaaagaaa tgagttcgac ccagtacttc cgccgcgtac ggtgtcaaca aacctgatat
     3661 agcggtaccg ccattgccta gcgcatagat tcctagagct aaaccgcgct gatttcttgg
     3721 aaaccaggcg ttgacgaacg gcacgccggc ggcaaacgaa gccccagcta tacctagtaa
     3781 taccgctgct aagaacagct ggttttcact atctactagt ggcaaccaga aaactaccgc
     3841 cgccgacaag aagcacatga ctgtgaataa ttttcgacca ccatatcggt cactcagtag
     3901 tcctagcggt atacgcccca gcgagccgat catcaccggc gccgccacta gcagcgacaa
     3961 catgaacgga gaaagtgaaa attccttggc ataggtcatc gccaacggtg aaatcaatga
     4021 ccaagcccaa aagttaatca acagtaagcc agtggcggca attagagcga cgtacttttg
     4081 atgtttttgg ctggttgttt ttgtcatatt ggtttctgtg attatatgcc gttaatcata
     4141 ccaactaacg ctttagtttg ctacgagttc aaatgaaact ttacctaagc ggtgcggcgt
     4201 atggagcttg gtgattgctg aaatacccaa ataagaatgc ccaagctagg attactacga
     4261 caatcactac tatccagata taagtccaat atcctttgac cggttcggaa atcagctccg
     4321 gaggtcgatg gatcggccgc ccttcatagt tgacatcttg ctctttggca attcttctgt
     4381 cgccattatt cataatcaga tcgacgcctc gttatcaaac aaaatcacat cctcacgata
     4441 gccgccgcgg ccaccataga gatgcttgta atctttgaca aactcaattg agcgaatata
     4501 tttgaccatt ttatagccta gtttacgctc cactcgcagg cgcaagggcg cgccgtgctc
     4561 tagtggcagc gacttccagt tcatttcgta ggccaaaatt gtgtatttat cgttcatatc
     4621 actcaggcgc agggcctcgt aaaacggttt gcctccagga taaagatcgt aggcgtgaaa
     4681 gacgacatat ttggcctccg agcttggctt agcccgttcg agtatttcgc gcatatggac
     4741 gccgccccac tcaccaatgg cactccatcc ctggatacaa atgtgtttgg taatctgttc
     4801 ctgtttgggc atttgtttga gctcgtcgag acttaacgat aactcgttct cgaccagtcc
     4861 ctcgaccttt aagcgccaat ctttaaagcc gttgtcacgc aggttataaa attcttcggt
     4921 gtcgggcggc agaccgttta tccgaaagaa gctggaaata tcttcttttt tgtaggtttt
     4981 gcgtggcgtc atccagccaa aaacggtatg aataaccggg ttcagtatgc caaccagcat
     5041 ctgccgcgtc cggacccgat ggttgacggt gaagtaggta atgacgatat gaaaaaccaa
     5101 gacaaacagt agaccggcca taaacagtgt cagcgcggcg gcaaagttta cgccggtgtg
     5161 gccaaaggta ataatctgga cgttgcggta aaaataaacc aacgccacca ttattacgtg
     5221 gacgatgata aagacactaa acagcaccat accgataaag tgtagcgacc gcgccgcttg
     5281 gcggcgatta ccaaacaata gtaagtattt aggaaaacga ttcatcagcg ccggactcat
     5341 ggccgccgcc gtcaagatca tcaaaactgg tagcaaaaag gcgaccgcgg cataagtcag
     5401 ttgctgcaat ggatcgtatg gctgaaacgc ctcaactggc ggtcggtgaa atgacaagta
     5461 gttcagtaga tcgtgccagg catcagggaa aacgctccag ctggtcggaa tcaaccgtcg
     5521 ccaagctccg gtcgcaaaca tgtagcccca gtagaccgca ccagtcgcca gccaaataat
     5581 tgccgctaca aaatgccagt tgcgggcatt gccaaaatcg tgatgttcgc cgccaggcaa
     5641 ggcccagtga ccaatctttt cggcttcatc gtgcgcttgg taaacgtggc cttccgggat
     5701 ttctttttta ccgaatttta gccaatagtt atcgcgacgg gtgtcatctg tccaataaag
     5761 aaatgggtga tccgccagaa tgtgcaagcc actgcgaatc agcaggagca gcacgattaa
     5821 gtttacgaag tgcgtaataa cgataactag cggcattgag tcgtccatgt ctacaagtat
     5881 actcgattat ggttggtgat atatgataat attatcatat gagatatgtg gtttttgatg
     5941 atttctttgc agtgctgggt aatcctcagc gggttagaat cttgcagtat ctaaaccgac
     6001 acggcagtaa gaacgttagc cagctttgtg aaaaattagg cctcgaacaa tcagccgtct
     6061 cacatagctt gcggcgacta agccgttgtc acttcgtcga gtgtgaaact cgcggcaaag
     6121 aacggattta cagcatcaat aaagatacgg tgcagccgct gtttaatttg attgacgaac
     6181 atgtgcgcaa atactgcgtc catggttgcg aacactggaa cattaaaaca aaggaggtta
     6241 tacatgccag tcgttaatgt taaattactt aaaaaagagc aggtcgccaa cggcactatg
     6301 gcttttcatt ggcaaaaacc cgaaggtttt gagttccgag ctggtcagtt ttgcgacata
     6361 acgctaatcg atccgccgga gactgatagt gagggtgacg tgcgaggatt ctcactggct
     6421 tacgcgccgt acgaaaatga cattgtcacg gctacgcgga tgcgtgatac ggcatttaaa
     6481 cgggtcttaa aagatttaca ggaaggcacg gaggtcaaat ttgacgcgcc atatggtgac
     6541 ttcactcttc acaaaaccga atcgacaccg gctgtcttta tcatcggtgg tatcggcatc
     6601 acgccggtcc gcagcatgat agcccaggcc actcacgacg aaacttctca taagattacc
     6661 ctgttatttt caaataaaac gccggccgat gcgccgttta ttagtgactt aagaggtttt
     6721 gctgaagcta accctaactt cactttcgtg ccggtctata ccgacgcagc cgaggccgaa
     6781 tgggacggtg agcgtggtta cgtcgatgcc gagatactga agcgccacgt ggctgaccta
     6841 agcgcaccaa tctactatct ggcggggcct gccggcatgg tcaaagccat gcgtcaaata
     6901 ctagtcgagg ctggcgctaa tgaagacaat atccggactg aagagttttc tggatactaa
     6961 aggttggcta gaaattaaaa tgtgaccagc tggtcacatt ttaatttggt gcgggtggag
     7021 ggagtctaac cctcgtcgcc tgcttggaag gcaggaataa taaacgttat accacacccg
     7081 ctcagtatga gacaaaacta cgttttttct catcggccag tcggcagagc cgaatttagc
     7141 taagctaaat caagctagcc tgctctttta ccttaatggc atgggtaatt ataccagatt
     7201 acttatcgga tttgatgtca tcgacagtga tgacgatagt atcaccggct tgggcttcac
     7261 ccgccagtag cttcttcgcc atgaccgact ctacagtccg ctggacaacg cggcgtaacg
     7321 gtcgggcgcc gaggcgcggg tcatacccta ggtcgactag cttggtctta gcttcatcat
     7381 cgacggtcag tctgaccttt tggtgagcca tagtcttatt gatgccggtg atgatgagat
     7441 cgataacttg cagtaattct tttttgtcga gcggtcggaa caagacgatc tcatcgaacc
     7501 ggttcaaaaa ctccggccgg aattgtttag agctaatcag ctcatcggtc aacaggtctt
     7561 caaagtcttc cagcttttca ccagagtcga tgtgatgacg gatctgatcg gcgccagcgt
     7621 tggatgtagc tataaatatg gcgtcacgga aagacacttc acgacccttg atatcacgca
     7681 agaccccctc gtccaaaacc tgcaataacg tcgttagtac cgcatcgtgg gccttttcca
     7741 actcatcgag taggattaca ctaaacggtt gcttcatgac ctgtgcagtc agactatgct
     7801 cgtcggctgc gccatcggca atcaatcttt tgacatcatc gggcgccaca aactcattca
     7861 tatcgagtcg aatcagtcga tcttctccgc cgaaatatac cgcggctaaa cttcgcgcca
     7921 attcagtctt gccgacaccg gtcgggccta gaaaaaggaa tgtaccgatc ggtttatttt
     7981 cgttcctaac tccggcccta gcccgtctga gagcatccga caccactcca acggccctgg
     8041 tctgattgat catccgctca tgaatcaagt cttcaagttg gagtagcttt tgcttttctc
     8101 ctgcatcact agtggcgcct atcttaacac cttgtccctg ttcaaccgct ctttgcactg
     8161 aaccagccgt caccaaaccg gactcggcgt agtcagcagc tgattcgagt agttttaggg
     8221 actttcccgg ctgtgcttga tcatacatgt agcgttcgct caggcgataa gcttcttgga
     8281 gtgcctggta catatatgta accttgtgtt tgaattcata gctgatcaat tgatcttgca
     8341 tgaccagcat ggtctcgtca cgattagtcg gttcgacacc aacacgattg agagcattgg
     8401 caagagccgg attacgagcg gctatcttga gccagcgctg ctcttccata gttaggatca
     8461 tacgcaaatt gccaccttcg ataaccggca gtaacacgtt cgacaggtca accgaaccga
     8521 cgccgtcttc gaagaacagc tccgctccgt ctaagcaaac tatgatgttc ttagcgcggt
     8581 aagcttcgat caaaagcgca tttactagct cctctaagtc acctctcccc ttagcgctgc
     8641 cgatcaaact accggcatct agattaacga cttggtggaa ccgcaaatta gccggcagat
     8701 ccactgacgg attcatcaac ttctcggcaa atgcatacac cagacttgtc ttgccaacgc
     8761 cgagaggtcc gactagggca atgttctgcc gaccaccgcc aacaaaagtc tgttgcaatc
     8821 gttcaagtgc tagcttatgt gcctccaagt caacactcaa caaactgcca ccttcgactt
     8881 gccgactaac attggctgcg aaacgttcaa ggagtggtgt atagccaaaa gtccagtcac
     8941 gcgccaaacc gccagtccgc ttcggttttt gatgatctaa aatcagccga tcaagatgat
     9001 cataccaacc agctccgttc gctacatcag cttcgtcgag ttgcaagtgt ggcaagatat
     9061 ttttaaaacc gttctgtacc gacactagcg cggctacaag atgagcaccg ctgacttgac
     9121 ctttacccgc caattgtcgg gccttctgcc aaatagcttc gaccgattga ctagcttccg
     9181 aggacaaatc agtcaaaaag ttaggcgcaa gaccaagtcg taccgccata aatctacctc
     9241 cgttagactg catggtagcc gtggccaagg ccttagctga caagtctcgg ggtaaagccg
     9301 ctaagacact ggcctccagc tgctgatcta gccgatcacc gatctcgacc ggcaacctcg
     9361 ataggtgtcg tcgttgccag accaaggtac agtaaggcca ggccgccagt gtcaaagcga
     9421 gccagccgat cggctcggca aatccaagaa gtagtaagcc gcagagtatc aaaactatag
     9481 ccgcgacccg aagcgacact atgatagaag gtcgtaattg ggcgccaaat cgagcttcga
     9541 ccgcgcgcct agagtgataa tcaaacatag ggactccaga ccagccagcc actagctgtg
     9601 agtatgacac cgattaccgg tacccaaggt aggatcggcc aaataactag ccatgtcagt
     9661 ccggcaaaag cagtgccgac aaagaccgct agccccacca gtatcatgat tgaacgaacc
     9721 ataactccga taaaccgcga gatcagtcga tctaaccaag cgcgtagtcg gacccccaac
     9781 gggccgccca ctttgacggt atcgagctgt cgaaacggtg agaatagact actactcaaa
     9841 ctacctatag accactgatc ggccgttcta gctaagcgtt cgccaaacag atttagttgg
     9901 tcacgccaac caaggccata ccaccaagag aaaaatccca ccaaaagcat aagttaattg
     9961 tatcaaacac tggtcgaagc aaggtataat tatctaagta tggatttgtc tcataaacta
    10021 gctctgactg ccggtcggct agttaaaaaa accgctagac tacgtggcgg tggctcagcc
    10081 ttacctggtt tggtcatcga aaaactggac ccgggcttcc taaagcgagc tctggctgac
    10141 ttgcctcgtg gtgtagcggt ggtctccggt actaatggca agaccaccac taccaaaatg
    10201 gtagtgcaac ttttgagaga tcaaggtctc aaagtcttta ccaacccgac aggttccaat
    10261 ttcacccgag gcgtggcagc agccctgata gaccaggtca atcccgagac cggcaagctc
    10321 gacgccgaca ttgcagtact ggagctagac gaagctcatg ccgtgcactt catccgcgaa
    10381 gttaaaccca gctatagctt gattctaaat atactaaggg atcagctgga tcgcttcggt
    10441 gagattgatc atactgccaa gttactagcc cgcgttgtcg agtcgactca atctggtgtc
    10501 gtcttaaatc gcgaagatcc gttagttttg aagctggccg ataacgtacc cgacagcgta
    10561 aagctacagt actttggcct aaatccagat ctaaaggacc gcttcccatc cgaccaaacc
    10621 atgcgcggta gttaccagac agacacaccg gataaattac cgcctctaca ccgcaaagac
    10681 accattttag acagactcga cggccggcag gcagtatttg cttttggctc gaagaaccaa
    10741 tcaccagtcg agctcaaact agatggtcta tataacgtcc tcaacgccgc agcggcgctc
    10801 ggtttagttc gactcatcct aggcgacaaa gccgacgaaa ctaagcttat gagtgaactg
    10861 gccgaagtta aaccagcctt tggtcgcggc gaagcagttg agctaagtga cggtaaatta
    10921 gaaatagttc tcgtcaaaaa tcccagtggc ttccgcctag gcctgaagtc ctattctgac
    10981 tcaccggccg ctactatgat ctgtataaat gataattatg ccgacggtcg cgacatgagc
    11041 tggctctggg acgtcgattt tgatcaattc gccaacgaca acaaggttgg agtaaccacg
    11101 gtaagcggca caagggccta cgatatggct ttgcgcctgc aatacgacgg tgtcctagtt
    11161 ggccatgtcg ataccgactt aggtaaagcc ttgaagcaat tcttgaccga cactacaggg
    11221 cagcggcgta tttacactac ctataccgcc atgctggagt tac
//
LOCUS       DASSBW010000054         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1019192, whole genome shotgun sequence.
ACCESSION   DASSBW010000054 DASSBW010000000
VERSION     DASSBW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019192"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1613
                     /locus_tag="VFG56_01565"
     CDS             93..1613
                     /locus_tag="VFG56_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622600.1"
                     /translation="MDLDRLMERVFAHGVGISRSYALTTERYRELADRSVKHPYHDSE
                     RTAEVHYLGERLADRAFKNLPKSIRDQAQAWPQMTVEEEQATLDQLMICLRSTHWRCL
                     APTISTVAGAESLPAQTGHWTRGGVMPNCLGVAQMLVGFARATGAEHMLVNVLEEADY
                     TRTKLLYGTIDWLCEILGTVPDCPERDAAIKRYDKVFDGILRDLDVMSKREAHHALLI
                     KTQRGWWLVDPYLQYKYRLLMRQAGPSAKFCRNHPGRVAYMNQPITRQGMRDLRLAEY
                     SSVFALAATREGLKPDQLLDNAAWSVYVSMMREYLPDKTKLAVKFRTVKGRLGKLGRM
                     REAQRRASYFQGSVLFSYDERKVAQDWPLAEYETRFDQRIEWLKTDQVFASKAAVRYF
                     DHALNQLLWWYSDCQSKGYGRHGLVELVEPCHGLASATLNHVAKASRLQVPGLVGLNA
                     SQWLMLDNLAIVERSGDCTARKIMNDRLERLSTLESVLVHPQFRIRKELVNGSAQQ"
     gene            1630..1800
                     /locus_tag="VFG56_01570"
     CDS             1630..1800
                     /locus_tag="VFG56_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622601.1"
                     /translation="MREEYEKVQSFDELREKFPDFSDAIDQLEREQKKAPKRRGSGST
                     RPIASRLKVPPR"
     gene            1983..2573
                     /locus_tag="VFG56_01575"
     CDS             1983..2573
                     /locus_tag="VFG56_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HET6622602.1"
                     /translation="MSEVPEDKPVVITSFEELTNVQSAIIGLNYQIDTANAKRDERIG
                     RAKDIYDFVARVETESAAETTDPLEAERDQLREAIATYLMKGRRRLRHRFGKTIESLF
                     GTVKWRVIAASVETPRDTSEVINHLLNRRGGKRYLRVRYDLDKKALLKAPDSLKRSLR
                     PFGVYIGKQETLTMQLKGESEPTTLRKYRFPRLRHQ"
     gene            2641..2964
                     /locus_tag="VFG56_01580"
     CDS             2641..2964
                     /locus_tag="VFG56_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622603.1"
                     /translation="MKSRLRGRTMYEDISDFTDEERRAVYYYELLRERADMGILYGED
                     RITYDWANQVLARRQAQATPPVAMVGYGYFTLVPGHERFQLKLMAGVLGAILILIAIA
                     NLFFS"
BASE COUNT          686 a          780 c          874 g          652 t
ORIGIN      
        1 atagaaaata gcaagttctt ttagattgca ataataagca atatatttta taatcagcca
       61 atcagcggac tttaacatct aaggagtaag caatggacct tgataggctc atggagcgag
      121 ttttcgcgca tggagtcggt atttcgaggt cgtatgcttt aacgaccgaa agataccgcg
      181 agctggccga ccgatcggtt aagcacccgt accacgacag tgagcgtacg gccgaggttc
      241 actacttggg cgaacgcttg gctgaccggg cctttaagaa cttgcccaaa tccatccgtg
      301 atcaggctca ggcttggcca cagatgacag tggaggagga gcaggctaca ctcgatcagt
      361 tgatgatctg cctgcgctcg actcactggc ggtgtttagc cccgaccatc tcgaccgtcg
      421 caggcgccga atcgttgccg gctcagaccg gtcactggac gcgtggtggc gtcatgccga
      481 actgtctggg cgtagcccag atgctggtcg gctttgcccg agcaaccgga gccgagcata
      541 tgcttgtcaa cgtactggag gaggccgatt atactcggac caagcttctc tatggtacga
      601 ttgactggct ttgtgaaatt ctgggtacgg ttccggattg cccggagcgg gacgcggcca
      661 tcaagcgcta cgacaaggtc tttgacggca tcctgcgtga tctggacgtt atgtcgaagc
      721 gtgaggctca ccacgcgctg cttatcaaaa cgcagcgcgg ctggtggttg gtcgatccgt
      781 acttgcaata caaataccgt ttgttgatgc gccaagccgg gccatccgcc aaattctgcc
      841 gtaaccatcc aggccgggtg gcttatatga atcagccgat taccaggcaa ggcatgcgtg
      901 acttaaggtt ggctgagtac agttcggtat tcgccttggc ggcaaccaga gaaggcctaa
      961 agcctgatca attactggat aacgccgcct ggtcggttta cgtgtcgatg atgagggagt
     1021 acctgccgga caagaccaaa ctggccgtta agttcaggac ggtcaaggga cgcctaggta
     1081 aacttggccg tatgagagag gcccaaagac gggcatcgta tttccagggt tcggttttgt
     1141 tcagctacga cgaaaggaaa gtggcgcaag actggccact ggctgagtac gagaccaggt
     1201 ttgaccaacg gattgagtgg ctgaagaccg accaggtctt tgcttccaag gccgcggttc
     1261 gttatttcga tcatgctctc aaccagttgt tatggtggta tagcgactgc caaagtaaag
     1321 gctacggccg ccacggactg gttgaactgg tcgaaccgtg tcatggtttg gcctcggcca
     1381 cacttaacca cgtagccaag gcgtcccggt tgcaagtacc ggggctagtc ggtctaaatg
     1441 cttcacaatg gctaatgctc gacaaccttg ctatcgtaga gaggtcaggt gattgtacgg
     1501 cccggaaaat catgaatgac aggctcgagc gtctctcgac gctagaatct gtccttgtac
     1561 atccgcaatt tcgaatccga aaggagctag tcaatggctc agctcagcag tagggcccaa
     1621 gtggcggcca tgcgcgagga gtacgagaag gtgcagtcgt tcgacgagct gcgcgagaag
     1681 ttcccggact tctctgacgc gatcgaccag ctggagaggg agcagaagaa ggcgcccaag
     1741 cgacgcggct ccggcagtac gcggccgatc gccagccggc tgaaggttcc gccccgctga
     1801 cacgcggccc caggatggga tggtgcaact cgcaccatcc catccgggcc gctttttaat
     1861 ttgcttttat ataacttgtc ttttgatttg tgcaaacggc ttaagcttgg cttgagggct
     1921 ctcaaggggt atagttttag gcgctagact tttctacgta cctactagac ttaggagtat
     1981 gcatgagtga agtaccggaa gacaagccgg ttgtcatcac cagcttcgag gagctgacca
     2041 acgttcagtc cgccatcatc ggtctgaact atcagatcga cacggccaac gccaagcgcg
     2101 atgagagaat cggtcgagct aaggacatat atgacttcgt cgctcgggta gaaaccgaaa
     2161 gtgcggccga gactaccgat ccgctggaag ccgagcgcga ccagttgcga gaggctatcg
     2221 caacttacct gatgaagggc cgacgcaggt tgcgtcaccg ctttggtaag accatcgaga
     2281 gccttttcgg aaccgtgaag tggcgggtaa ttgccgcttc ggtggagaca ccgagagaca
     2341 ccagtgaggt gatcaaccac ctgctcaacc gccgtggcgg caagcgttac ctgcgagtgc
     2401 gctatgatct ggacaagaag gcgctcctca aggcgccgga cagcctcaag cggtcgctca
     2461 ggcctttcgg tgtctacatc ggcaagcaag aaacgctgac gatgcagctg aagggtgagt
     2521 cggagccgac tacccttagg aagtatcgct tcccgcgtct tcgtcatcaa tgaaggtcta
     2581 gcaaggccct gggttttacc acccagggct ttgttaattt gtgccaaaag tggtataact
     2641 atgaaatccc gactgagagg acgaacgatg tacgaagata tatcggattt cacagatgag
     2701 gagcggcgag cggtctacta ctacgagctg ctgcgcgagc gcgccgacat gggtatcttg
     2761 tacggcgaag accggatcac ttatgactgg gccaatcagg tgttggctcg gcgccaagct
     2821 caggcgactc cgcccgtggc tatggtcggc tacggttact tcacgcttgt accgggtcac
     2881 gaacgattcc agctcaagct gatggccggc gtgctcgggg caatcctgat tctcatcgcc
     2941 atcgccaatc tcttcttttc ctaaaccggg aggcccgcac tgatcaggtg tt
//
LOCUS       DASSBW010000055         9030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1766605, whole genome shotgun sequence.
ACCESSION   DASSBW010000055 DASSBW010000000
VERSION     DASSBW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766605"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="groEL"
                     /locus_tag="VFG56_01585"
     CDS             complement(<1..924)
                     /gene="groEL"
                     /locus_tag="VFG56_01585"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6622604.1"
                     /translation="MSKQVFYDDDARTRVLGGAKALYDAVKVTMGPKGRNVVIQKSYG
                     HPTVTHDGVTVAENVELPVGEDETLGYGVGADLIKQAATKMNKAAGDGTTTVTVLTYH
                     ILNEANRLIAAGHNPMELKKGVEAAGAEVLSALEAVKEPISGKKSRVAEVATISAGDS
                     EIGQLIAGVIDKVGADGVVTVEEGQSLNMESEVVEGFTFDRGYVSPMMVTDTARMEAV
                     YEKPAIVVTDKKVSSVQEFLPLLEKLAAAGKKDVVLIAEDVEGEALGVLVLNKLKGVF
                     NTVAVKAPAFGDRRKEVLADIACLTGATVIGE"
     gene            complement(1028..1294)
                     /locus_tag="VFG56_01590"
     CDS             complement(1028..1294)
                     /locus_tag="VFG56_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET6622605.1"
                     /translation="MSVPISPLADRLVAVRDKAEAKTASGLYLPDSAKEQPVVATVVA
                     VGPDVKAVKKDDKIVYKEYSTTELKVGEAEYLIVKEEDVLATLN"
     gene            1472..1975
                     /locus_tag="VFG56_01595"
     CDS             1472..1975
                     /locus_tag="VFG56_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622606.1"
                     /translation="MQNTDGKGSPKIPASSEEQNGIRRAKKAQQRRQKSSHTTASTYV
                     SDRELTRQEVVEVHIEMLQQAAPMVVPQPPEEQKIFLDAFLVAYRKMSFADLHVGTKF
                     SPLQAIIAEVERKLDQKDLQRHRDNWIHAQARFRQAWQAAPYRDARRNYDEDVQQELA
                     RRLINVA"
     gene            2140..2415
                     /locus_tag="VFG56_01600"
     CDS             2140..2415
                     /locus_tag="VFG56_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622607.1"
                     /translation="MTETTTPLSPEQHKAYTELSKEAQSAEDLVTLREMAEADAGTGE
                     AHHIEVMGEDGEVQTVQVTDIGKGALKHTLDQSAVAVHSESWQNDQR"
     gene            2503..2826
                     /locus_tag="VFG56_01605"
     CDS             2503..2826
                     /locus_tag="VFG56_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622608.1"
                     /translation="MNIGVCGYTRATAQSVASDLNHLAQHPELGFVRLDVTYNRTASG
                     SRVPDEHSASYWVTFNNETTTLVLANNSELVQLKTKEGRRLDIIFPRAAERELQPARI
                     LIDDE"
     gene            complement(2875..4161)
                     /locus_tag="VFG56_01610"
     CDS             complement(2875..4161)
                     /locus_tag="VFG56_01610"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET6622609.1"
                     /translation="MKEELVKAARRVLPKPAIKGLETVYRQNRARAVAAKYGYPARSL
                     RVIAVTGTNGKTTTANYLNEILKANGHTTALFSTAQIELAGKKTLNDLNVTVATVDQM
                     QRFFRDAKAAKADYVILEATSHALDQYKLAGVPIECAIMTNLTQDHLDYHGTMDNYAA
                     AKAKLFKMKPEFVVLNRDDDWFDYFNQFDGESQKITYGTDDQADARITKVKLYKKGSE
                     VEVTVDHQTELKLATALPGKFNVYNMSAAAAAAYLMHIDIEAIEQGVQNLEGVAGRFE
                     SIDVDKPYEVIVDYAHTPDALEKLLETIKSTTKNRMILVFGACGDRDKLKRPIMGRIA
                     ADLADRIVLTDEESYNEDPTEIRQMIMDGIVDGKGKAKTDEIEDRKEAIKKAMNIARK
                     GDVLVITGMGHEQFRVVKGKKIPWNDGEVVRELAQK"
     gene            4192..5271
                     /gene="tsaD"
                     /locus_tag="VFG56_01615"
     CDS             4192..5271
                     /gene="tsaD"
                     /locus_tag="VFG56_01615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET6622610.1"
                     /translation="MMLILGIESSCDETAAAVVRDGQELLSNVVNSQIDIHAAYGGVI
                     PEIAARSHIEVIMPVIEQALLHARVTWDDIDAIAVTHAPGLIGSLLIGSLAARTIALI
                     KQKPLYGIHHVESHTYANFLTSGIKLSIPYPESQPQFPLLSLTVSGGHSQLVLFEDHG
                     KYRLLGQTRDDAVGEAFDKVAKIIGLPYPGGPSIAKAAETGNTNKYDLPKARMENPYD
                     FSFSGLKTAVLRAVQRECGVDHTFPSFKLSERLSQVQQSDFAASFQRVAVETLVDKTV
                     KAYKEFQPKSVVIAGGVAANQELRRQLKDRLPTVIAYAPMELCTDNGAMVATLGYYQA
                     KLRSPDNPLTLSVVPSLSMTKTSWQ"
     gene            5413..7944
                     /locus_tag="VFG56_01620"
     CDS             5413..7944
                     /locus_tag="VFG56_01620"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET6622611.1"
                     /translation="MKLAKVYNPTEYEPTAYALWEASDAFKPSGEGKPYSVVMPPPNA
                     NGNLHMGHALTLDLQDILIRWRRMKGDRTIYIPGADHAGFETWVVFEKELAKQGKSRF
                     DFSREELYSQVWDFVESKRGAMELQSRALGASASWDDLVFTLDDKVIKTVNKTFKRLW
                     DDDLIYRGERLVNYCTFHQTGFADIEVEHDDAKTPLYYMKYGPFEVATTRPETKFGDT
                     AVAVHPDDERYKQYVGQEIEFDGLNGPVKLQVVADKMVEREFGTGVVKITPAHDFNDF
                     EVAERHNLPSLKVIGLDGKLTSVAGQYAGMTVADARAAVVADLKAKGLLVRVDEDYHN
                     RIAKCYKCGTVIEPLLLEQWFLKVEPLAKRAQQAIDEGQINFTPKSKARLITQYLDNL
                     KDWNLSRQIPWGIPIPAFVNINDETDWIFDERVHLPTIEIDGQTYKREEDTFDTWFSS
                     GQWPFVVTDFLDNGELAEFYPTDMMETGHDLIDRWVARMIMLGLYTTDNIPFKNVYLH
                     GMVLDENGQKMSKSKGNVINPMDVIADYGSDALRLGVVANRSAGQNQAFARDKVIAGR
                     NFCNKLWNMARFIEPYLDGQDVTQPAAKTVADQWVVDRLLAARDTIDEHLGVYRFAEA
                     ADTVYHVIWNEVADWYLEAAKTNPDPAMLAWVLETCLKLAHPFAPFVTETIWTTLERS
                     DIPLISTTWPLDLDRRPLEAATFEGIRQLVSEIRYVASDLPGGKQTLLFENDRLVGDN
                     RELIGQLARLKQVKQTTQPQGIRLAVPNREAWLAVDEEVLYEHQTKLEVRLADTRQQI
                     DKLKTRLANANYVAQAPAKIVDETRRQLADNQQLAERLQSELDVL"
     gene            complement(7930..8244)
                     /locus_tag="VFG56_01625"
     CDS             complement(7930..8244)
                     /locus_tag="VFG56_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622612.1"
                     /translation="MTVMLASVHAKDLVSYSKLSLIGGAAVITLTDVGSKALSNLHNH
                     VDNTSFNHFIQVVHRRQLSREREDERDEALKNQQGVLDRLIASWRLATASLFMRVSLK
                     NV"
     gene            complement(8348..8800)
                     /locus_tag="VFG56_01630"
     CDS             complement(8348..8800)
                     /locus_tag="VFG56_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET6622613.1"
                     /translation="MQIRIVAIGPKHDPQLALKLSEFEKRLPYSVDWQLLAYSKAEGD
                     TARRAESAHILDKITERDLVILLDETGSQLTSDALADKVMAWQAGGRRLTFVIGGAYG
                     VDKGLKDRADFVWSLSQLVFPHQLVRLILVEQLYRASAIASGHPYHHR"
     gene            complement(8801..>9030)
                     /locus_tag="VFG56_01635"
     CDS             complement(8801..>9030)
                     /locus_tag="VFG56_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622614.1"
                     /translation="DYQAVTATVKKVAKRLRDVLGRAYVGEMVVGTDVPHAHVHVVAF
                     DKAAEIKHVIVREDGQADSAVLAAMAERLAF"
BASE COUNT         2242 a         2396 c         2184 g         2208 t
ORIGIN      
        1 ttccccgatc accgtcgctc cagttaggca agcgatgtca gctaagactt cttttcttcg
       61 gtcaccaaaa gctggggcct tgacggccac tgtattgaaa acacctttga gtttgttaag
      121 aaccagcaca cccaaggcct caccttcgac gtcttcagcg atgagaacca catccttttt
      181 accggcggcg gctagttttt ctaagagtgg tagaaattcc tgaacggaag agactttttt
      241 gtcggttacc actatggctg gcttttcgta aaccgcctcc atccgagcgg tatcagtcac
      301 catcattggg ctgacataac cacggtcaaa agtgaaacct tcaaccactt ctgattccat
      361 attgagactt tgaccttctt cgacagtgac cacaccgtca gccccgacct tatctataac
      421 accagcaatc aattggccta tttcagaatc acctgccgaa atggtagcga cttcagccac
      481 ccggcttttc ttgccagata tcggttcttt aacggcttcc agagcactca aaacttcggc
      541 accagccgcc tcgacacctt ttttcaattc catcgggttg tgaccggccg cgatcaggcg
      601 gttggcttcg tttaggatat ggtaagtcag aaccgtcacg gtagtcgtgc cgtcaccagc
      661 agctttgttc atcttggtag cagcctgctt tatcagatcg gctccgacac cgtagcccaa
      721 agtctcatcc tctcccactg gcagttcgac attctcagct accgttacac cgtcatgagt
      781 aacggtcgga tggccgtagc tcttctggat aaccacattg cgacctttgg ggcccatggt
      841 tactttgacg gcatcgtata gcgctttggc gccacccaac accctagtgc gggcatcgtc
      901 atcataaaaa acttgtttac tcatctatcc tccttatatc tctagactat ggacgttgga
      961 cgatagacgt tagatgatga gttccattca cccagcatcg caatgcctaa cgtctatctt
     1021 ctaccgtcta atttaaagtt gctaagacgt cttcttcttt gacgatcagg tattcagcct
     1081 cgccgacctt cagctcggta gttgaatact ctttatagac aattttgtca tctttcttaa
     1141 cggctttgac gtctggaccg acggctacga cggtagccac aaccggctgt tctttagcgc
     1201 tgtctggcag atagaggcca ctcgcagtct tagcttcggc cttatcgcga acggcgacca
     1261 gacggtcggc taatggtgaa attggtacgc tcatcccctc tccttcttta aatatctaac
     1321 ttgttatgtg tactaattgt agccgactaa attagcactc gtcaactcag agtgctaagt
     1381 attagagaca tgtagtattg acatagttta tagattatgg tagtatttac aaatcaccaa
     1441 aaggtgtgta cccaagtcct gggaggacat catgcagaac accgacggta agggcagtcc
     1501 gaagattccc gcgtccagcg aggagcagaa cggcatccgc cgggccaaga aggcccagca
     1561 gaggcgccag aagtccagcc acaccacggc cagcacctac gtctccgacc gcgagctgac
     1621 tcgtcaggag gtggtggagg tccacatcga gatgctgcag caggcggcac cgatggtggt
     1681 gccgcagccg ccggaggagc agaagatctt cctcgacgcg tttctggtgg cctaccggaa
     1741 gatgagcttc gccgatctcc acgtcggcac caagttctcg ccccttcagg ccatcatcgc
     1801 cgaggttgag cgtaagctcg accagaagga tcttcagcgc caccgcgaca actggatcca
     1861 tgctcaggct cgttttcgtc aggcgtggca ggccgctccc taccgggatg cccgccgcaa
     1921 ctacgacgaa gacgtccagc aggagctggc ccgacgtctg atcaacgtgg cctaacgggc
     1981 cgacgggtac ggccaggggt gagccaagac aagctcaccc ctggccagaa catattttat
     2041 tgagtcgctt tcaagcggct atttttatat atacttgact ctatgtaaat attgtgctaa
     2101 tgtttaaagc agtacaaagc taaaacaaaa aggttaaaca tgacggaaac caccacccct
     2161 ttaagccctg aacagcacaa agcttacacc gagctctcaa aagaagctca gagcgcggaa
     2221 gacttagtta ctctgcgcga aatggctgag gccgatgctg gcacgggtga agcccatcac
     2281 atcgaagtta tgggtgaaga cggcgaagtt caaaccgtcc aggttacaga cattggtaag
     2341 ggtgccctta agcacaccct cgatcaatcg gccgtagctg ttcactctga aagctggcag
     2401 aacgaccaac ggtaatagta aaaaataaaa aaaatagctc acttgaggtg ggctattttt
     2461 tatgttataa tataaaagcc ctttaccaag gaggtgagaa ggttgaatat cggcgtatgc
     2521 ggctatacgc gggcgacggc tcagtccgtt gctagcgact tgaaccacct agcccaacat
     2581 cccgaactcg ggttcgtccg actggacgtt acctacaatc gcacggcttc gggtagccgc
     2641 gtgcctgatg agcatagcgc ctcatactgg gtgacgttca acaatgaaac aaccacactg
     2701 gtactagcca acaacagtga gctggtgcag ttgaaaacca aggaggggcg acgtctcgac
     2761 atcattttcc cgcgggccgc tgagcgtgag ctccaacccg ccaggatact gatcgacgac
     2821 gaatgaccgg tagccccgcg acggccttac agtcgtcgcg gggcaccggt ccgtttattt
     2881 ttgagctaac tctctgacga cttcaccgtc gttccagggg atttttttgc ctttgacgac
     2941 gcggaattgt tcatgaccca tacctgttat aaccaagaca tcgcccttgc gagctatgtt
     3001 catggctttt ttgattgctt ctttacgatc ctcgatctcg tccgtcttag ccttaccttt
     3061 gccatcgaca ataccgtcca tgatcatctg tctgatctct gtcggatcct cgttataact
     3121 ctcttcgtcg gttagaacga tgcggtcagc caaatctgct gcaatccgtc ccatgatagg
     3181 acgcttgagt ttgtcgcggt caccgcaggc tccgaatacc agaatcatgc ggttcttagt
     3241 agttgacttg atagtctcta gtaatttctc tagagcatca ggggtgtgag cataatcaac
     3301 gataacttca tagggcttat cgacatcgat tgactcaaat cgccctgcta caccttccag
     3361 attctggaca ccttgctcaa tcgcctcaat atcgatatgc atcagatagg cggctgcggc
     3421 tgcggcagac atattgtaga cgttaaattt accaggcagc gccgtggcta acttaagttc
     3481 ggtctggtgg tcgacggtca cttcgacctc actacccttc ttatacaatt tgaccttagt
     3541 tatccgagca tcggcctgat cgtcggtacc gtaggttatc ttctggctct cgccatcgaa
     3601 ctggttgaag tagtcaaacc agtcgtcgtc gcggttgagg acgacaaact ccggcttcat
     3661 cttgaacagc ttggctttag cggcggcgta attgtccatg gtgccgtgat aatccaagtg
     3721 gtcttgtgtc agattagtca tgatggcgca ctctatcggt acaccggcca gtttgtactg
     3781 gtcgagagcg tgtgatgttg cctctaagat gacgtaatcg gccttggccg ccttggcgtc
     3841 acggaaaaaa cgctgcatct gatcgaccgt agctactgtg acattcaggt cattgagcgt
     3901 ctttttaccg gccaattcga tctgggctgt tgaaaacaac gccgtagtgt gaccattggc
     3961 ttttaatatt tcattcagat aattggcggt ggtagttttg ccgttggtgc cagtaacggc
     4021 tatcacccga aggctgcggg caggatagcc atatttggcc gccacagccc tggcccgatt
     4081 ttgacgatat accgtctcaa gccctttgat tgccggcttc ggtaagacac ggcgagcggc
     4141 tttgaccaac tcttctttca taaatccatc ataccacctg ctacactagt aatgatgtta
     4201 attttgggaa tcgaatcctc atgtgatgag actgctgctg cggtagttcg tgatggccaa
     4261 gagttgttgt ccaatgtcgt caattcacag atcgacatcc atgccgctta cggcggtgtc
     4321 ataccggaaa tcgccgcccg ctcacatata gaagttatca tgcccgtcat cgagcaggcc
     4381 ttactccacg cccgagttac ttgggatgat atcgatgcta tcgccgtcac gcatgcaccg
     4441 ggccttatcg gttccctgct tatcggcagc ttagccgcta gaactatagc tctgatcaaa
     4501 caaaaacctc tttacggcat ccatcacgtg gaatctcaca cttacgctaa ctttttgact
     4561 tctggcatca agctttccat tccctacccc gagagtcaac cacaatttcc cctactcagc
     4621 ttgaccgtga gcggtggcca ttcgcaattg gtcttatttg aagaccacgg taaataccgc
     4681 ttacttggtc agacccggga tgacgccgtc ggtgaagcat tcgacaaggt agccaagatc
     4741 atcggtctac cctaccctgg cggcccatca attgccaaag cggccgagac aggcaacacc
     4801 aacaagtatg acttgcccaa ggctaggatg gaaaaccctt atgattttag cttctctggg
     4861 cttaagacag ccgttctaag agctgttcag cgcgaatgcg gtgtggatca cacttttcca
     4921 tcgtttaagc tctctgagcg tctcagccag gttcaacaat ctgactttgc cgctagcttt
     4981 cagcgcgttg ccgtggaaac cctagtcgac aaaaccgtaa aagcctacaa agaattccag
     5041 cccaaatctg tcgttatcgc tggtggcgtc gctgccaatc aagagttgcg tcgtcagcta
     5101 aaggaccgcc tacctaccgt aatcgcctat gcaccgatgg agttgtgtac cgataacggc
     5161 gctatggtgg ccacactcgg ctattatcaa gccaagctcc gctctccaga taaccccctt
     5221 accctttcgg ttgtccctag tctgtcgatg accaagactt cttggcagta agcagctggc
     5281 ctaaccgcta gcgaaggcta atgtttctat tttcctagtg cggggaacgc aatccctcga
     5341 aagtaacaat agcgtcatct aaggtgttac aataacaggg aatcaacctc tttaattttt
     5401 aatgtgaaaa atatgaaatt agccaaagtc tacaatccaa ccgaatacga accaactgcc
     5461 tacgctttat gggaggcgag tgacgccttt aaaccatctg gagaagggaa accttacagc
     5521 gtcgtcatgc caccgcctaa cgccaacggt aacctccaca tgggtcatgc gctgaccctg
     5581 gatttacagg acattctgat ccgttggcgg agaatgaaag gggacaggac tatttacatc
     5641 cccggcgcgg accatgctgg ctttgaaaca tgggtagtat tcgaaaaaga gttggccaaa
     5701 caaggcaaat cacgctttga tttttcacgc gaagagttat attcacaagt ttgggatttc
     5761 gtcgaaagca aacgcggtgc tatggaactg caatcgcgcg ctctcggtgc cagcgccagc
     5821 tgggatgatc tagtgtttac ccttgacgac aaggtcatca agacggtcaa caagacgttt
     5881 aagcgactat gggacgacga cctgatatac agaggtgagc gcttggtcaa ttactgcacc
     5941 ttccaccaga ccggtttcgc cgatattgaa gttgagcacg acgacgctaa aacaccgctt
     6001 tattacatga aatacggccc gttcgaggtt gctacaactc gaccggaaac taaattcggt
     6061 gacacagcgg tagccgtcca tcccgatgat gagcgttata agcaatacgt cgggcaggag
     6121 attgagttcg acggcttgaa tggcccggtc aagctccagg tagtcgccga caagatggtc
     6181 gaacgcgaat tcggtaccgg agtggtcaag attacgccgg ctcatgattt taacgacttc
     6241 gaagtggctg aacgtcacaa cctacctagt ctcaaggtta ttggtttaga cggcaaacta
     6301 accagtgttg ccggtcagta cgccggtatg accgtagctg atgcccgagc cgccgtggta
     6361 gccgacctaa aagccaaggg tttactggtg cgagtcgacg aggactacca caaccggatc
     6421 gccaagtgtt acaagtgtgg caccgttatc gaacctttgc tgcttgagca gtggttcctt
     6481 aaggtagaac cactcgctaa acgggcccaa caagccatcg acgaggggca aatcaacttt
     6541 acaccgaaga gcaaagcccg gttgatcacg cagtacttgg acaacttaaa agactggaat
     6601 ctgtctaggc agataccatg gggtattcct atcccggctt ttgtaaatat caacgatgag
     6661 accgattgga tatttgacga gcgggtgcac ttaccaacta ttgagattga cggccagacc
     6721 tacaaaaggg aagaagacac tttcgacact tggttctctt ctggtcaatg gccgtttgta
     6781 gtcacagact tcttggataa cggagaatta gccgagtttt atccaacaga tatgatggag
     6841 actggtcacg acctgattga ccgttgggta gctcgcatga tcatgctcgg cctctacact
     6901 acggataata tcccgttcaa aaacgtttat ctacacggta tggttcttga cgagaatggg
     6961 cagaagatga gtaagtccaa gggcaatgtc atcaacccga tggacgtcat cgccgactac
     7021 ggttcagacg ccctacgtct cggcgtagtt gccaaccgct cagcgggaca aaatcaggcc
     7081 tttgcccggg acaaagttat tgctggtcgt aatttctgta acaagctctg gaacatggca
     7141 cgtttcatcg aaccttacct tgatggtcag gatgtcacac aaccagcagc taaaaccgtc
     7201 gccgatcaat gggtggtcga ccgtcttcta gcggcgcgcg acaccatcga tgaacaccta
     7261 ggcgtgtacc gcttcgccga agcggctgat accgtctatc atgtgatttg gaatgaggtg
     7321 gccgactggt acttagaagc tgccaagacc aaccccgatc cagccatgct ggcctgggta
     7381 ttagaaacgt gtttgaagct ggctcatcca tttgcgccat tcgtgactga gactatctgg
     7441 acaaccctag agcgctccga tataccattg atatcaacca cctggccgct tgatttggat
     7501 cgacgaccac tagaagccgc tacttttgaa ggtatccggc aattagtcag cgagatccgc
     7561 tacgtcgcta gtgatctgcc tggtggtaaa caaacgctgt tatttgaaaa cgaccgtcta
     7621 gttggcgata accgagaact gatcggccaa ttagcccgcc tcaagcaggt taaacagacc
     7681 acccaaccac agggaattcg cctagccgta cccaatcgcg aagcttggct ggcggtcgac
     7741 gaagaagtgc tctatgagca ccagaccaag ttggaagtcc ggctagccga tacccgtcag
     7801 caaatcgata aactaaagac tcggttggcc aatgccaact acgttgctca agctccagcc
     7861 aaaatcgtcg atgagacccg taggcagctg gctgataacc agcagttggc tgagcggctt
     7921 cagagcgaat tagacgttct ttagactgac gcgcataaac aggctggctg tagctagacg
     7981 ccaagaggca atcaagcgat caagtacccc ttgttgattt ttcagcgcct cgtcacgctc
     8041 atcctctcgc tctcggctta gctgacggcg atggaccact tggatgaaat gattgaacga
     8101 tgtattgtcg acatggttgt gtagattaga cagagcctta gaacctacgt cagtcaaagt
     8161 tataaccgcc gcaccaccga tgagcgacag cttgctgtaa ctgactagat ctttggcgtg
     8221 gacactggct agcattactg tcatacacag acagtaggct agtaagctaa gtgagatttt
     8281 gatagttttt gttttcataa ttgactttat gcaatattag cataagtttt acataatgtc
     8341 aattagctta tctatgatga tatggatgac cactcgctat agcgctagct cggtacaact
     8401 gctcaactag tatcagtcgg actagttgat gcgggaaaac cagctgggac aacgaccaga
     8461 caaaatcagc tcgatctttc agacccttat cgacaccgta agctccgccg ataacaaaag
     8521 ttagccggcg acctccggct tgccaagcca taaccttatc agctagagca tctgaagtaa
     8581 gctgtgagcc ggtttcgtca agcaggatga ccagatctcg ttcagtgatt ttgtcgagga
     8641 tatgagcgct ctcagctcgt ctagccgtat cgccctccgc cttagagtag gctagcaact
     8701 gccagtcgac agaataaggt aggcgtttct caaattcaga cagcttaaga gccagttgtg
     8761 ggtcatgctt aggcccgatg gcaacgatac ggatttgcat ctagaaggct aatctctcag
     8821 ccatagcagc taacacggcc gaatcagcct gaccgtcttc acggacgata acatgcttga
     8881 tttcggctgc cttatcgaat gcaaccacgt ggacgtgagc atgtggcaca tcggtaccga
     8941 ccaccatttc tccgacataa gccctgccca ggacgtccct gagtcgcttc gcgactttct
     9001 taaccgtggc ggtaaccgct tgatagtcat
//
LOCUS       DASSBW010000056         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1223450, whole genome shotgun sequence.
ACCESSION   DASSBW010000056 DASSBW010000000
VERSION     DASSBW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223450"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFG56_01640"
     CDS             complement(<1..520)
                     /locus_tag="VFG56_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6622615.1"
                     /translation="MKPTISAEALAVTKSNKPILNNLDFEIKQGSLNGLIGPSGAGKT
                     TLIRAIVGIQRLSSGRLTVLGAAAGEPKLRSKIGYVTQSPSVYADLTVRQNIEYFAKL
                     LRAPKSDVDKVIDQVHLRPQADQVVSSLSGGQHARVSLAVALLGNPEILVLDEPTVGL
                     DPLLRQDLWQLFK"
     gene            complement(657..733)
                     /locus_tag="VFG56_01645"
     tRNA            complement(657..733)
                     /locus_tag="VFG56_01645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(697..699),aa:Ala,seq:ggc)
     gene            complement(846..1286)
                     /locus_tag="VFG56_01650"
     CDS             complement(846..1286)
                     /locus_tag="VFG56_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HET6622616.1"
                     /translation="MTYINKTSIQATIHCLTGCAVGEVTGMVVATALGWHNVAQIALS
                     VFLAFLFGYSLTMWPLIKKMSLKAALAVALVADTVSITVMEIVDNGFVALVPGALNAV
                     LSDWLFWWSLGLSLVVAFILAVPVNGWLISRGKGHAVVHHHHHH"
     gene            1607..1867
                     /locus_tag="VFG56_01655"
     CDS             1607..1867
                     /locus_tag="VFG56_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6622617.1"
                     /translation="MNYVSKKLFIGSLAWATTDDSLKAHFEQCGDVASAKVITDRESG
                     RSRGFGFVEFDDDKAADTAVEKLNGSDLDGRSINVSEARPRE"
     gene            complement(1942..>2502)
                     /locus_tag="VFG56_01660"
     CDS             complement(1942..>2502)
                     /locus_tag="VFG56_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET6622618.1"
                     /translation="QVTKLDRFAEISAGKLVDAIGSKKQPSLNRFIYGLGIRHIGTQT
                     AIDLANRFRSLDRLTEATLEDLAAVEGIGDVVAEAVAEYFAEPRNQQLLEKFKASGVW
                     PVEVKQIGGKLSGVNFVITGTLTSMSRDEAAEKIRDLGGTFQTSVAKDTNYLVVGGKV
                     GASKLKKAQDYGTEVIDEAKLLELFA"
BASE COUNT          643 a          625 c          580 g          654 t
ORIGIN      
        1 ctttaaataa ctgccataga tcctggcgca acagcggatc aagtccaact gtcggttcat
       61 ccaaaactag tatttcaggg ttaccgagca aagccacggc tagcgacacg cgagcgtgtt
      121 ggccgccaga caaagagcta accacttgat cggcctgagg cctcaagtga acctggtcta
      181 tcaccttgtc cacgtctgat tttggtgctc taagcaactt ggcgaagtac tcgatattct
      241 gcctgaccgt caggtcagcg tagacacttg gactttgggt gacgtagcct atcttagaac
      301 gtagctttgg ttcgccagcc gcagcaccaa gtacggtcaa tcggccagag ctcaggcgct
      361 ggatgcccac gatggctcgg atcaaagtag ttttgccggc tccgctagga ccgattaaac
      421 cgtttaagct gccttgttta atttcaaagt ctagattgtt taagataggt ttgtttgatt
      481 ttgtaaccgc caaagcttcg gctgagatag ttggcttcat atcgcttatg atagcataat
      541 atcccatata acttcgtcac ttgctcgaag cgagcttctg cgcgagttcc ttgttatcgg
      601 atttagacaa atccgacatg caagtaaaac accgtccaga cggacggtgt tttacttggt
      661 ggagcatatc ggatttgaac cgatgacctc ttccatgcca tggaagcgcg ctagccaact
      721 gcgccaatgc ccctcgtcag agtgaggtct tttgcttaca aataaaaact atgagttttt
      781 atttgggtta gccgcaaaaa cgactacaag gacaaatgat attttaacag aggttatttc
      841 aaatctcaat ggtgatggtg gtggtggacg acggcgtgac ctttgccgcg ggatatgagc
      901 catccgttaa ccggtaccgc caagataaat gccaccacca acgacaaccc taagctccac
      961 caaaatagcc agtcgcttaa gacggcgtta agcgctcccg gcactaaggc gacgaagccg
     1021 ttatcgacga tctccatgac tgttatcgac accgtgtcgg ctacaagagc taccgctaat
     1081 gccgctttca ggctcatctt cttgatcagc ggccacatag tcagactgta gccaaacaaa
     1141 aacgctagaa atacactcag agctatctgg gcaacattgt gccagcctaa agctgtagcc
     1201 accaccatgc cggtaacttc acctacggca cagccggtga gacagtgtat ggtagcttga
     1261 atcgaggttt tgtttatgta tgtcatgatc ttaagatagc atatatagcg tcacctctca
     1321 atacaaaagc gctatatata gcgcttttgt ataaattcgc tggtgtgcgc ccacccgggg
     1381 cggtcagttt caaaagtttt tgtttttata actagctgcc ccaatggcac accagtcctc
     1441 gggtctaaat ataccctaag cataaagctt ttggcaaatt gtcgcttttt tgtcacattt
     1501 atgcttaaat aaaagtgttt cagagagcgc aaattgttga acatagagaa ttacacaatc
     1561 cacgattcaa ccttcgctgg tcgaactgaa gttaagacaa cgaaggatga attacgtgtc
     1621 taaaaagtta tttattggta gcctagcttg ggcgactacc gacgacagcc ttaaagccca
     1681 cttcgaacag tgtggcgatg ttgctagcgc taaagtcatc accgatcgtg aatcaggccg
     1741 cagtcgcggt tttggtttcg tcgaatttga cgacgacaag gcggctgaca ctgctgtcga
     1801 gaagctaaac ggctccgacc tagatggtcg atcaatcaac gtttcagaag ctcgccctcg
     1861 cgaatagcca agattgatct ccagtgtaaa aacggccttt actggtcgtt tttatttttg
     1921 cgtcgcaatc cgttgactgg gctaagcgaa caactctagt agcttggcct cgtcgataac
     1981 ttcggtaccg tagtcttgag cttttttgag cttggatgct ccgactttcc cgcccaccac
     2041 aagatagtta gtgtctttgg ctaccgatgt ttgaaaagtg ccaccgagat ctctgatctt
     2101 ctcagccgct tcatctcgac tcatggaggt gagggtgcca gttattacaa aattaacacc
     2161 agataacttg ccaccgattt gtttgacctc caccggccag acaccggaag ctttgaattt
     2221 ctctagtagt tgctggttgc gcggttcagc aaagtactcg gccaccgctt cagccaccac
     2281 atcgccaata ccctctactg cagccaggtc ttcgagagta gcctcggtta accggtcgag
     2341 actacgaaaa cgattagcca gatctatagc ggtttgcgtc ccgatatggc ggatacccaa
     2401 gccgtaaata aaacggttga gcgatggctg ttttttagat ccgatggcgt cgactaattt
     2461 acccgccgag atttcggcaa acctatcaag cttagtcacc tg
//
LOCUS       DASSBW010000057         8203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1479348, whole genome shotgun sequence.
ACCESSION   DASSBW010000057 DASSBW010000000
VERSION     DASSBW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479348"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFG56_01665"
     CDS             complement(<1..143)
                     /locus_tag="VFG56_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET6622619.1"
                     /translation="MPRKATKSLQRRLNPDRRYQSVLVARLINKSMYDGKKHIAEKAV
                     YSA"
     gene            complement(146..559)
                     /gene="rpsL"
                     /locus_tag="VFG56_01670"
     CDS             complement(146..559)
                     /gene="rpsL"
                     /locus_tag="VFG56_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET6622620.1"
                     /translation="MPTINQLVRKPRKSAKKKSKSPALGRIHNTLKTRYYDQGSPLKR
                     GVCVKVTTKTPKKPNSALRKVARVRLTNGQEVWAYIGGEGHNLQEHAVVLVRGGRVPD
                     LPGVRYHIVRGALDLQGVSDRKQGRSKYGSKKEGK"
     gene            complement(829..4665)
                     /gene="rpoC"
                     /locus_tag="VFG56_01675"
     CDS             complement(829..4665)
                     /gene="rpoC"
                     /locus_tag="VFG56_01675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET6622621.1"
                     /translation="MARLVTDTDIADFDAVRLVVASDDDIKKWSYGEVTKPETINYRT
                     QKPERDGLFCERIFGPVKDINPHDAKLKGIRSREAAVDKNGELVTKSVVRRERMGHIG
                     LAVPVAHIWFMRGAPSAMSLLLGVPVRALERVAYFASYIITEVDETKRDQMMTDAEAE
                     HEAAQNAIKLRYEKEAESEGADIKALAEARNRELDDLNEAYELKKSQLDSLRKRALIS
                     ENDFRELPEDYQDLVEVGMGGQALYKLLKEIDLAKLIEELTAEVETAKGQRKKKLMKR
                     LKVLEGMHNAGINPTSMCMSVLPVIPPDLRPMVQLTGGRFATSDLNDLYRRVINRNNR
                     LKKLIDLNAPEVIRRNEMRMLQEAVDALIDNSAARGGRAVSATGGRRRLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVAGPELKINQCGLPKAMALELFKPFVISWLLEHEHA
                     HNIRSATRLIDAGEAVVWDALDEAIKDKYVLLNRAPSLHRLSIQAFQPKLIEGKAIQL
                     HPLVCTGFNADFDGDQMAVHLPLSKQAQKEARELMSATNNLLKPADGSPILNIAQDIV
                     LGCYYLTYPKPSAQTDKVRSYGSTAEAVYAFDKGQIKLQTPIRLFAKGEMRDTTLGRV
                     YFNEVLPADFPYVNDTMSKKALKKVMARIFNRYGSQMTADTADIIKDLAFKYATSAAV
                     STGMTDYFDVPELDGIIAQGDARTAAIADQYEQGLITEEERYNLTTETWRDIDKQVTD
                     VLNEKIKGEDTSIAVMVDSGARGDIGNIKMASAMIGVMVDATGNEIELPIRSSFKKGL
                     SPLEAFVATRGSRKGLIDTALKTADSGYLTRRLVDVSQDIFTVVDEEGDDPGFSIYRS
                     ESEETQVPFGDRLYSRYAAADIAGHVKAGELITRDIADAIENDPKVEEAKIMSALSST
                     NLRGVPQKSYGLDMATGQLVDQAQPVGVIAAQSVGEPGTQLTLRTFHAGGVAGQDITQ
                     GLPRVDELFEARTPKGEAVLSKQAGRVQVWEDGDTYVVQVTPESGKVERIKLDGQIVR
                     VKSGSEISAGDVLASNEDESLPIVAPFEGTVEVGKGELVVTSSAEAPARYEVPGYRQL
                     TVKDGDQVEAGGRLTTGSINLHDLMALKGVEATQRYIVNEILRIYAAQGQNIADKHLE
                     IIVRQMFSRVQIEDAGDSLFVTGDIVSKAAVIETNRQLTGEGKEPAQFTQLLLGITKV
                     SIWSDSFLSAASFQDTTRVLINAATSGRVDHLYGLKENVIIGRKIPVGTGAQIDEPTE
                     LEAEDVETDEIVPEEA"
     gene            complement(4665..8018)
                     /locus_tag="VFG56_01680"
     CDS             complement(4665..8018)
                     /locus_tag="VFG56_01680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET6622622.1"
                     /translation="MAKAKANQDRVYFTQSDAALDMPNLISHQLKSWQEFVETGLGEI
                     FAEVSPIVDYTEQKLSLSFKDYHFEDPKMSESEAKENNVSFEAPLKVNAELTNKVTGE
                     VKTQEIYLGDYPWMTERGTFVINGVERVVVSQLIRSSGIFFTADNIGGRNQYGAKLIP
                     GRGAWLEFETATNGAIYVKIDRRRKIPITTLLKALGHSSEKSIKELFKNVDTGEVKYI
                     DATLEKDPARATNDALIEVYRRLRPGDLATVDNARSMIERMFFDFKRFDLSRVGRYKM
                     NQRLGMDVANTAENRVMRMEDLIAIISEIIRLNNTQEPADDIDALHNRRIKLVGELVA
                     RQFRIGMLRMDRNAKDRMSVSEIETVTPGQLINARPVVAAVREFFASSQLSQFMDQTN
                     PLSELSHKRRFSSMGPGGLSRERAGLEVRDVHPTHYGRICTVETPEGANIGLVLNMAT
                     YARINEYGFLEAPYRKVKNGKVSDEVVYLDASEEAAEIIAEGATELSADGKFKEDRVA
                     ARVNLVPGEVDAAEVTYMDAAHKQVIGSTAALIPFLEKNRIDRSLTGSNMQRQAVPLI
                     KTQSPIVGTGVEGVIARNSSQLVTAEKAGEVVRADADEVHVKYGPKEVKVYKPMHFIK
                     SNDDHSINQKVVVHRGDKVKAGDALIEGASVADGELALGRDLVVAFMPWGGYNMDDSI
                     VISRRVVQDDDLTSINIKDFVVEVRETKLGPEIVTRDIPNVSEESLRHLDEDGIVRVG
                     AEVKGGDVLVGKITPKGEQELSSEERLLRAIFGEKAKDVRDTSQRMQNGKQGKVVGVK
                     VFSRDQGHELKAGVLMQVQIFVAEMRKVRVGDKLAGRHGNKGVVARVLPVEDMPFMED
                     GTAVDVVLNPLGVPSRMNLGQLFETHLGMAARALGYKVATPPFNGVPNDVISSELKKA
                     GLPEDGKVQLFDGRTGEAFQEKTTVGDMYMIKLHHMVDDKIHARSIGPYTMVTQQPLG
                     GKAQNGGQRFGEMEVWALEAYGAATTLQEMLTIKSDDVYGRAKAYESIIKNEPITGPK
                     IPESFNVLVKELQGLGLRIDLVDEAETIDAEPVLENDNEDETIPAVTASETEAVGDEA
                     DEFGDIELGQAVSIQEAEEVTEEETKEAN"
BASE COUNT         1791 a         2189 c         2133 g         2090 t
ORIGIN      
        1 atggcggaat aaacggcctt ttcggcaatg tgtttcttac cgtcgtacat agacttgttg
       61 atcaaacgag ccaccaatac gctttggtaa cgacggtccg gattcaagcg gcgttgtaga
      121 gatttggttg ctttacgcgg catgattatt tgccctcctt cttagagcca tacttagatc
      181 ggccctgttt gcggtcagac acgccttgaa ggtctagcgc accgcgaacg atgtggtaac
      241 gcacacccgg aagatccggc actcggccgc cacggaccaa tactacggcg tgctcttgta
      301 ggttgtgacc ttcaccaccg atgtaggccc agacttcctg gccgttagtt aggcggacac
      361 gagcaacttt acgaagagcc gagttcggct tcttcggggt cttagtagtt accttgacac
      421 agacaccgcg ctttaggggt gaaccctgat cgtagtaacg ggtctttagg gtgttatgaa
      481 tccgacccag cgccgggctc ttactcttct tcttggcgct ttttctaggc ttgcggacca
      541 attggttaat ggttggcact ttaaaaatct cctggattag gattactaaa ttttgaccgc
      601 ttctcttagg tgataagtct aatcgagcga taatctacgg cggttttcag aattaaaccg
      661 acccgtcttt ctcctggcgc tgacatctaa gtgttgtaca cgttcactta gtcagtcaga
      721 agaggaaact catgagacat cttagcattt tgccgagagt ttgtcgagag cttgccaacc
      781 aaaagcccgc cgtagacaag gcgggctttg gcgatttaga ctgtgccctt aagcttcttc
      841 tggtacaatc tcgtcggttt cgacgtcttc tgcctctagc tcggtcggtt catcgatctg
      901 ggcaccagtt ccaactggaa tcttgcgtcc gatgatgacg ttctccttca agccatacag
      961 gtggtcgacg cgaccagagg tggcggcgtt gatcaacacc cgagtggtgt cttggaagga
     1021 agcagcggac aggaagctgt cgctccagat actaaccttg gtgataccaa gtagtagctg
     1081 ggtaaactgt gccggctctt tgccctcccc agtcagctga cggttggttt caatcactgc
     1141 agccttggag acgatgtcac cggttacgaa caagctgtca ccagcgtctt cgatctgaac
     1201 tcggctaaac atctggcgaa cgatgatctc caggtgcttg tcggcgatgt tctgaccttg
     1261 agccgcgtag atacgcagga tctcgttgac gatgtagcgc tgggtggctt cgacaccctt
     1321 cagagccatc aggtcatgca ggttgatcga accggtagtt agccgaccac cggcttctac
     1381 ttggtcgccg tccttgacgg tcaactgacg gtagccaggg acttcgtaac gagctggtgc
     1441 ttcggcgctg gaagtaacga ctaactcacc cttaccaact tcgacagtac cttcaaacgg
     1501 tgccacgatc ggtagtgatt cgtcttcgtt ggaagccagc acgtctccgg ctgagatctc
     1561 gctgccagac ttaacgcgca cgatctgacc gtctagcttg atccgctcaa ctttaccgga
     1621 ctcaggagta acctggacca cgtaagtgtc accatcttcc cagacctgga ctcgaccagc
     1681 ttgcttcgac agaaccgctt cacccttcgg tgtacgggct tcgaacagct catcaacacg
     1741 cggtagaccc tgggtgatgt cttgaccggc cacaccaccg gcgtggaagg tacgtagcgt
     1801 cagctgggta cccggctcac cgactgattg agcggcgata acgccgaccg gctgggcctg
     1861 gtctactagt tgaccggtag ccatgtccag accgtagctc ttctgcggta caccgcgcaa
     1921 gttggtggac gacagagcac tcatgatctt ggcctcttcg acctttggat cattttcgat
     1981 agcatcagcg atatcacggg tgatcaactc tccagctttg acgtgaccgg caatgtcagc
     2041 ggcagcatag cggctgtaca atcggtcacc aaacggcacc tgggtttctt cactttccga
     2101 acggtagatg ctgaaacctg ggtcgtcgcc ttcttcgtct actacagtga agatgtcttg
     2161 agagacgtca accaaacgac gggttaagta accggagtca gctgtcttaa gcgccgtgtc
     2221 gatcagaccc ttacgtgaac cacgggtggc cacgaaggct tccagtgggc tcaggccctt
     2281 cttgaagcta gaacgaatcg gcagctcgat ctcgttaccg gtagcatcga ccatcacacc
     2341 aatcatggcg ctagccatct tgatgttacc gatgtcacca cgagcacctg agtcgaccat
     2401 gacggcgata gaagtatctt cgcccttgat cttttcattt agcacatcgg tgacctgctt
     2461 gtcgatgtcg cgccaagtct cggtagttaa gttgtagcgc tcttcttcag tgatcagacc
     2521 ttgttcgtac tggtcggcga tagcagccgt gcgggcgtcg ccttgggcga tgataccgtc
     2581 gagctcaggt acgtcgaagt agtcggtcat accagttgaa acagctgcgc tggtagcata
     2641 cttgaaggct agatccttga tgatgtcagc cgtatcagcc gtcatctgag aaccgtagcg
     2701 gttaaagatc cgagccatga ctttcttcaa ggcttttttg ctcatagtgt cattgacgta
     2761 cgggaaatcg gccggcagga cttcgttgaa gtaaacccgt ccaagcgtag tgtcacgcat
     2821 ctcgcctttg gcgaacaagc gaattggtgt ctgcaatttg atctgaccct tgtcaaaagc
     2881 gtagacggct tcagcggtcg aaccgtagga acgaacctta tcagtctgcg ccgacggttt
     2941 cggataagtc aggtagtaac agccaagtac gatgtcctga gcgatgttca agatcggcga
     3001 accatcggct ggttttagca ggttgttggt agcactcatc agctcgcgag cttctttctg
     3061 ggcctgcttg gacagcggta agtggaccgc catctggtca ccgtcgaagt cagcgttgaa
     3121 accggtgcag actagcgggt gaagctggat agctttacct tcgatcagct taggctggaa
     3181 ggcctggata gatagacggt gcaacgacgg cgcacggtta agtagtacgt acttgtcctt
     3241 gatagcctca tcaagcgcgt cccaaaccac agcctcgccg gcgtcaatca gacgagtagc
     3301 cgaacggatg ttgtgagcgt gctcgtgttc gagcaaccag ctgatgacga atggcttaaa
     3361 cagttctagt gccatcgcct taggtaggcc acattgattg atcttaagct ctggaccagc
     3421 cacgataacc gaacgacctg agtagtcgac acgcttaccg agaaggttct ggcggaaacg
     3481 accctgctta cctttcagca tgtcggatag cgacttcaat cgacggcggc caccggtagc
     3541 actgacggcg cgaccaccac gggcggccga gttgtcgatc aatgcatcga cggcttcctg
     3601 cagcattcgc atttcgttgc gacggataac ttccggagcg ttaaggtcga tcaacttctt
     3661 cagacggttg tttcggttga taacgcgtcg gtataggtcg tttaagtcag aggttgcgaa
     3721 tcggccaccg gtcaattgaa ccattggacg taagtctggt gggataaccg gtaggacact
     3781 catgcacatg ctagtcgggt tgatgccggc attgtgcata ccctctagaa ccttaagtcg
     3841 cttcatcagc ttcttcttgc gctgaccctt agcggtctcg acttcggcgg tcagttcctc
     3901 gatcagctta gctagatcga tttctttcag tagtttgtaa agcgcctgac cacccatgcc
     3961 aacttctacc aagtcctggt agtcttctgg caactcgcgg aagtcgtttt cagaaatcaa
     4021 agctcgtttg cgtaggctat cgagttgact cttctttagc tcgtaggctt cgttcaagtc
     4081 atccagttcg cggttacggg cttcggctag agccttgatg tcggcaccct cggactcagc
     4141 ttctttttcg tagcgtagtt tgatagcgtt ctgagcagct tcgtgctctg cttcggcgtc
     4201 ggtcatcatc tggtcgcgct tggtctcatc aacttcagtg atgatgtagc tagcgaagta
     4261 agctactcgc tctaaggcac gaaccggtac gccgagtaat aagctcatag cactcggtgc
     4321 tccacgcata aaccagatgt gagccactgg tacggctagg ccgatgtggc ccatgcgctc
     4381 acgtcggacg actgacttgg tgaccaactc accgttcttg tcgacggcag cttcgcgcga
     4441 acgaatacct tttagctttg cgtcgtgcgg gttgatgtcc ttgaccggac cgaagatccg
     4501 ctcacagaac aaaccgtcgc gctctggttt ctgggtgcgg tagttgatgg tttccggttt
     4561 ggtaacctca ccataactcc acttcttgat gtcgtcatca ctggcaacga ccaggcgaac
     4621 tgcatcaaaa tcggcaatat cggtgtctgt tactaatctg gccattagtt tgcctcctta
     4681 gtttcttctt cagttacttc ttcggcctct tggatactga cagcttggcc gagttcgatg
     4741 tcaccaaatt catcggcttc atcgccaacc gcttcggtct ccgaggcggt gacggccgga
     4801 attgtctcat cttcgttatc gttctcaagt acaggttcgg cgtcgatagt ttcagcttca
     4861 tcgaccaggt cgatacgaag gcctagacct tgcagctcct tgactaacac gttaaacgac
     4921 tcagggatct tcggaccggt aatcggctcg ttcttgatga tcgactcgta agccttggct
     4981 cggccataaa cgtcgtccga cttgatagtt agcatttcct gcagagtggt ggcggcaccg
     5041 taagcttcta gggcccaaac ctccatctca ccgaaacgct gaccaccgtt ttgtgcctta
     5101 ccaccaagtg gttgctgggt aaccatggtg tatggaccga tagaacgggc gtgaatcttg
     5161 tcatcaacca tgtggtgtag cttgatcatg tacatgtcac cgacagtggt cttctcctgg
     5221 aaggcttcac cggtgcggcc gtcgaatagc tggaccttac cgtcttccgg caggccggct
     5281 ttcttgagct cgctcgagat gacatcattt gggacaccgt taaatggcgg tgtggcgacc
     5341 ttgtagccaa gagccctggc ggccatacct aggtgggtct caaatagctg gcctaagttc
     5401 atacgactcg gcacacctag tgggtttagg actacgtcta cggcagtacc atcttccatg
     5461 aacggcatat cttcaactgg taggactcgg gcaactacac ccttgttacc gtgacggcct
     5521 gccaacttgt caccgacgcg gactttacgc atttcggcta caaagatctg gacttgcatc
     5581 agtacgccgg ctttcagctc gtgaccttgg tcgcggctaa ataccttgac gccgacaacc
     5641 ttaccttgct taccgttttg catacgctgt gaggtgtcgc gaacatcttt ggctttctca
     5701 ccgaagatag cccgtagcaa gcgctcttcc gagctcagtt cctgctcacc ctttggtgtg
     5761 atcttaccga ctaagacgtc accgccctta acttcagcac cgactcgtac gataccgtct
     5821 tcgtctaggt gacgaagtga ttcttccgag acgttcggga tgtcgcgggt aacgatttcc
     5881 ggaccaagct tggtttcgcg gacttcgacg acgaagtctt tgatattgat gctggtcagg
     5941 tcatcgtctt ggacgacacg acgggagata acgatagagt catccatgtt ataaccaccc
     6001 catggcataa aggcaacgac taggtcgcga ccgagggcca attcaccgtc agccacagat
     6061 gcgccttcga tcagggcgtc accggctttg accttgtcgc cgcggtgaac aacaactttt
     6121 tggttgatag agtgatcgtc gttggacttg ataaagtgca tcggtttgta aacttttact
     6181 tccttcggac cgtacttgac gtgaacttca tcagcgtcgg ctcggacaac ttcaccggct
     6241 ttttcagcgg tgactaactg cgaagagtta cgcgcgataa cgccttcaac gccggtaccg
     6301 acgatcggac tttgcgtctt gatcagcggc acagcttgac gttgcatgtt cgaaccagtc
     6361 agcgacctgt cgatacggtt cttctctagg aacgggatca aggcagcggt tgaaccgata
     6421 acctgcttgt gagcggcatc catgtaggta acttcagcgg catcgacttc acctggcacc
     6481 aagttgacac gggcagcgac acgatcttct ttgaatttac cgtcagcact cagttcagtc
     6541 gcaccttcgg cgatgatctc agcagcttct tcggaagcat ctaagtaaac gacctcgtcc
     6601 gataccttac cgttcttgac tttgcgatat ggcgcttcca ggaaaccgta ctcgttgata
     6661 cgggcataag tcgccatgtt gagcaccaga ccgatgttgg caccttctgg agtctctacc
     6721 gtacaaatac gaccatagtg agtcgggtgt acgtcacgga cttctagacc ggcacgctct
     6781 ctagagagac caccagggcc catcgagcta aagcgacgtt tgtgcgatag ctcagatagt
     6841 gggttggtct gatccataaa ctgcgatagc tggctggacg cgaagaactc gcgtacggca
     6901 gctaccaccg gacgagcgtt gataagctga cctggtgtga cggtttctat ttcactgacc
     6961 gacatacggt ctttagcgtt acgatccatt cgcagcatac cgatacggaa ctgacgagca
     7021 actaactcac cgaccagctt gatgcggcgg ttgtgcaagg catcgatgtc gtcagctggc
     7081 tcctgcgtgt tgttgaggcg gatgatttcc gagatgatgg cgatcaaatc ttccatacgc
     7141 ataactcggt tttcggcagt gttggcgaca tccataccaa ggcgttggtt catcttgtag
     7201 cgaccgacac ggctcaaatc gaaccgtttg aaatcaaaga acatccgctc gatcatcgaa
     7261 cgagcgttgt cgacggtagc caggtcacct ggacgcaagc ggcgataaac ttcgatcagg
     7321 gcatcgttgg tagcccgagc tgggtctttc tctagggtgg cgtcgatgta tttaacttca
     7381 ccggtgtcga cattcttaaa cagttccttg atcgattttt ccgacgaatg accaagagcc
     7441 ttaagtagcg tagtgatcgg gatcttacgt cgacggtcga tcttgacgta gatagcgccg
     7501 ttggtagcgg tttcgaactc cagccatgca ccacggccag gaatcaattt ggcaccgtat
     7561 tgattacggc caccgatgtt atcggccgta aagaagatac cgctagaacg gatcaactga
     7621 gaaacgacga cacgctcaac accattgata acgaaagtgc cgcgttcggt catccacggg
     7681 tagtcaccca agtagatctc ttgggtctta acttcaccgg taaccttgtt ggtaagttca
     7741 gcgttgactt taagtggagc ttcgaagctg acattgtttt ctttggcttc gctttcgctc
     7801 atcttcggat cttcgaagtg ataatccttg aagctgagcg atagtttttg ttcggtgtag
     7861 tcgacgatcg gactgacttc agcgaaaatt tcgcctaatc ccgtttcgac aaattcctgc
     7921 cacgatttca gttggtggct gatcagatta ggcatatcca gcgcagcgtc ggactgcgtg
     7981 aaataaaccc ggtcttggtt agctttagct ttagccatag actcctttga tttagattaa
     8041 gttagtgatc cttagtacag cccgcttggg ttgaaggcta taccggcgcc gaagatctac
     8101 tagctttggg ctctgcacac aaaaacgccc cgccaaatgg cgtgcgtgca taaataccca
     8161 agcttacact gacttcatta cctacaacta tagctgccga cag
//
LOCUS       DASSBW010000058         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1506411, whole genome shotgun sequence.
ACCESSION   DASSBW010000058 DASSBW010000000
VERSION     DASSBW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506411"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..607
                     /gene="atpF"
                     /locus_tag="VFG56_01685"
     CDS             80..607
                     /gene="atpF"
                     /locus_tag="VFG56_01685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET6622623.1"
                     /translation="MVNVLAQLAADPAESGILGLNAQAFVVQLITFILAIVVLERFAV
                     KPILQVLDRRRQTIEKGVKLGEQMQQDKAKLDQKVTEELYKTRQKADQIIADAEAAAK
                     RRAVDIEAKTKDKVDSMLSSAEDRIRQESSAARRKLESEMAELVASAAGAVLREKVDA
                     KKDAKVIAAALESEA"
     gene            609..995
                     /locus_tag="VFG56_01690"
     CDS             609..995
                     /locus_tag="VFG56_01690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET6622624.1"
                     /translation="MAVKRSELAATINRHLTGSDKFDAKQIAAYLMSSGRERELDSLI
                     RDIMSLRADQGIVELTAISAHPLTGEALKRIEAIVKSVRPQAKTVVIDQEIRPGLIGG
                     VRLKMVHHELDLSLRGRLNQLKRKLV"
     gene            1014..2558
                     /gene="atpA"
                     /locus_tag="VFG56_01695"
     CDS             1014..2558
                     /gene="atpA"
                     /locus_tag="VFG56_01695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET6622625.1"
                     /translation="MSELSIKELSSDIRAAIEELKGRPEIEETGVVTRVGDGIAWIYG
                     LRSCGFSEMIDIDGVDGQKVTAFAFNLGEEEIGAVLLGADELVEAGASARSTGKVLQV
                     PVGPELVGRVVDPLGRPLDGGEPIKTQQFAPVEQTAPAVLDRKHVSEPLMTGLAAIDA
                     MVPIGRGQRELIIGDRQTGKTAIALDTMINQARQKTGIINVYVAIGQKSSKVARLVER
                     LKREKVMDQTIVVATSPADPASMLYLAPYAATSMAEYFRDNKGHALVIYDDLSKHAAA
                     YRQMSLLLRRPPGREAFPGDVFYLHSRLLERSAKLSDELGGGSITALPIIETQAGDIS
                     AYIPTNVISITDGQIFMETNLFYQGIRPAISVGLSVSRVGGDAQTKAVKSVAGTLRLE
                     LAQFRELASFSQFSTDLDPETKHQIERGQRLTELLKQPQYSPLSVWQMYASLYAATSG
                     CFDKVPVDKIKTAQAVLLDELEAKHKDLTKQLNTGDKPTEDEQKEITKVAEAVAKGYE
                     VKAEQA"
     gene            2575..>2996
                     /locus_tag="VFG56_01700"
     CDS             2575..>2996
                     /locus_tag="VFG56_01700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET6622626.1"
                     /translation="MANTTTIKRRINSIKNTRQITKAMELVASSKLRKAQNQAEVTRD
                     YRQTAYRLLARLSQVREVEQQPLFKKRPVKTKLYVLISSNSGLAGAYNSNVFKKLTSS
                     LEQDKANNVQAKVITIGSKGVQFVRQLKNIELEAHYPN"
BASE COUNT          767 a          739 c          801 g          689 t
ORIGIN      
        1 atctttacgg ctctggctga gttgttcggt ctaatcgctt ttgcgtcggt ctttatcgtc
       61 aaataaggaa aacttataga tggtaaatgt tttagcacaa ctagcagccg atccggccga
      121 gtcgggcatc ttgggcctca atgcccaggc ctttgtggtc cagttgatca ccttcatttt
      181 ggcgatcgtg gtgctggaaa ggtttgccgt caaaccgatc ttacaagtcc ttgatcgtcg
      241 tcgtcagacg attgaaaaag gtgtcaaact tggtgaacag atgcaacaag ataaggctaa
      301 gcttgaccag aaagtgaccg aagagctgta caagacgcgc caaaaggccg atcagatcat
      361 tgccgacgct gaagcggctg ctaaacgccg ggccgtcgat atcgaagcta agaccaagga
      421 caaggtcgac agtatgctta gttccgccga agaccggatc cgtcaagaat ctagcgcggc
      481 tcgtcgcaag ttagaatcag agatggctga attggtggct tcagctgccg gtgcggtctt
      541 gcgtgagaaa gtcgacgcta agaaagacgc caaggtcatc gccgcggcac tggagagcga
      601 ggcttaggat ggcagtcaaa cgttccgaat tggccgccac tatcaaccgt catctgaccg
      661 gctcggataa atttgacgct aaacagatcg cggcctatct gatgagtagt ggtcgtgagc
      721 gtgaactaga cagtttgatc cgggatatca tgagcctcag agccgaccaa ggtattgtcg
      781 aactaaccgc tatcagtgct catccattga ctggcgaagc gcttaagcgg atcgaagcca
      841 tcgttaaatc ggttcggcct caggccaaaa cggtcgttat cgatcaagaa atccgaccag
      901 gtcttattgg cggcgttcgc cttaagatgg tccatcatga gttagatctg tctttgcgcg
      961 gtcgactcaa tcagttgaaa cggaagttag tctaagggaa agaaaaggaa ttaatgagcg
     1021 aattatcaat caaagaatta tcaagcgaca ttcgcgccgc catcgaagag cttaagggcc
     1081 ggccggaaat cgaagaaacc ggtgtcgtca cccgggttgg tgacggtatc gcttggattt
     1141 acggtcttcg ttcctgtggc ttttccgaga tgatcgacat cgatggcgtc gacggacaga
     1201 aagttacagc ctttgccttt aaccttggtg aagaagaaat cggtgccgtg ctcctgggcg
     1261 ccgacgaatt agtcgaagct ggcgcttcgg ctcgctcgac cggtaaagtc ttgcaggttc
     1321 cggtcggtcc ggaattagtc ggtcgagtag tcgatccgct gggccgccca ctcgatggtg
     1381 gtgagccgat taagacgcag cagtttgctc cagtcgaaca gaccgcaccg gcagtattgg
     1441 accgtaagca cgtctccgaa ccgttgatga ccggtttggc cgctatcgat gccatggtac
     1501 cgatcggccg ggggcagcgg gagttgataa tcggtgatcg tcagaccggt aagactgcta
     1561 tagcactgga cactatgatc aaccaggcac gtcaaaagac cggtatcatc aacgtttatg
     1621 tggcgatcgg tcagaaatca tctaaagtcg ctcgcctagt agaacgcttg aagcgagaga
     1681 aagtcatgga tcagactatc gtggtggcga cttctccggc tgatccggcc tcaatgcttt
     1741 acctggctcc ttacgcagcc acttctatgg ccgaatactt ccgcgacaat aaaggccacg
     1801 ccttggtcat ctacgacgac cttagtaaac acgctgctgc gtaccgtcag atgtcactac
     1861 ttttgcgtcg cccgccgggt cgcgaagcct ttcctggaga tgttttctac ctgcactcac
     1921 ggttactgga gcgttcggct aagctatccg atgaactagg tggtggttcg atcacagccc
     1981 taccaatcat cgaaacccaa gccggggaca tcagtgccta tatcccaact aacgtcatct
     2041 ctatcaccga cggtcagatc ttcatggaaa ccaatctgtt ttaccaaggt atccgtccgg
     2101 ctatctcggt tggtttgtcg gtgtcccgtg tcggtggtga tgcccaaacc aaggccgtca
     2161 agagcgtggc tggtacgctg cgtttggagt tagcccagtt ccgggaactg gcttcctttt
     2221 ctcagttctc aactgaccta gacccagaga ccaagcacca aatcgagcgt ggccagcgct
     2281 tgaccgagct tttgaaacaa ccccagtata gtccgcttag tgtttggcag atgtatgctt
     2341 ctctttatgc cgctactagt ggttgtttcg ataaggtgcc ggtcgacaaa atcaagaccg
     2401 cgcaggctgt tttgttagac gagctggagg cgaagcacaa agatttgacc aaacagttaa
     2461 acaccggtga taaaccgact gaagatgagc aaaaagagat tactaaggtg gccgaagcgg
     2521 tagctaaggg ctacgaagta aaagctgaac aagcttaaag aggtttaagg acgaatggcc
     2581 aatacaacaa ccattaagcg gcggatcaac tcgatcaaga atactcgtca gatcaccaag
     2641 gcgatggagt tggtggcctc aagcaaacta cgcaaggcgc aaaaccaagc cgaggtgacg
     2701 cgcgactatc gacagacggc ctatcgtttg ttggctcgcc tatcgcaggt tcgtgaggtt
     2761 gagcagcagc cgttgttcaa aaagcggccg gttaagacca agctatatgt acttattagc
     2821 tccaatagcg gcctagcagg tgcctacaac tcgaacgtat ttaagaaatt gactagttcg
     2881 cttgagcaag acaaagcaaa caatgttcaa gccaaggtta ttaccatagg ctctaaaggc
     2941 gtacagttcg tccgacagtt aaaaaatata gagcttgagg cccactatcc taactt
//
LOCUS       DASSBW010000059         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_631254, whole genome shotgun sequence.
ACCESSION   DASSBW010000059 DASSBW010000000
VERSION     DASSBW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631254"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFG56_01705"
     CDS             <1..823
                     /locus_tag="VFG56_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET6622627.1"
                     /translation="GEKRRNNAEWAVAHLGKQVDTLITIPNDRLLQTIDRRTPLLETF
                     KIADDVLRQGVQGISELITEHGLINLDFADVKAIMANAGSALMGIGRASGDDRAAKAA
                     QQAIESPLIEVSIDGAKGVLFNVTGGYDMSMSEIQEAAEIITSAVAPDANIIFGATLK
                     PEMEDELIITVIATGFDSAYFHEQAAALAERDRPEEVGQTESTELDKPAEVETADVDS
                     INMDLDNDMADAVKDDFATESESTAIWQNVDEEEADTPAFLRRRKKNKKKDKDDK"
     gene            1039..1503
                     /gene="nrdR"
                     /locus_tag="VFG56_01710"
     CDS             1039..1503
                     /gene="nrdR"
                     /locus_tag="VFG56_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET6622628.1"
                     /translation="MKCPKCQHPDSKVIESREAGDGDSIRRRRECLNCHCRYTSYERV
                     ERPNLAVVKKNGDRELFDRNKLFGAIMVSVGKFLRSDVEVEQIVAKVEDELYALGTPE
                     VTSAQIGDKVLEELGSHNGVAYVRFASVYQNFSSLEGFERAIKQARELSSKE"
     gene            1569..>3160
                     /locus_tag="VFG56_01715"
     CDS             1569..>3160
                     /locus_tag="VFG56_01715"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HET6622629.1"
                     /translation="MAHTVSALNVERYFTSDGEHPYDQLTWAKRDSKIVNPGTGKTVF
                     EQKDVEFPEFWSQNAINIVTQKYFYGTPGTPQREQGLKDLIDRVVDTITKHGYDNGYF
                     ESKPESKTFSDELKYILGTQRAAFNSPVWFNIGTPDRSQQASACFILEVDDTLPSILN
                     WYREEGMIFKGGSGAGLNLSKIRSSKETLSSSGGHASGPVSFMRGADASAGTIQSGGK
                     TRRAAKMVILNIDHPDIEEFIWCKAREERKAFVLEQAGFEMGLNGRDMVSIQYQNANN
                     SVRVNDKFMKAVENNDDWELVGVKDKKVYDTVKARDLFRQVAEAAWESADPGMQFDTT
                     INDWHTTPNAGRINGSNPCSEYMHLDNSACNLASINLLKFLRNDGTYDIEAYKHTVET
                     IFLAQEILVGYSEYPTENIGKNAKAYRELGIGYANLGATLMAKGLPYDSDEGRAFAAT
                     VTAILTGHSYATSARIAKRVGTFDGYADDEKGMIRVLRKHRDAVRDIDASLVPEDLLN
                     AAAGVWDDAVELGEKYGVRNSQA"
BASE COUNT          862 a          804 c          803 g          691 t
ORIGIN      
        1 gggtgaaaag cgccgcaaca acgccgagtg ggccgttgct cacctaggta agcaagtcga
       61 cacgctcatc actatcccaa atgatcgact gcttcagacc atcgatcgtc gtacgcccct
      121 gctcgagacc ttcaagattg ccgatgatgt gcttcgtcaa ggtgtgcagg gtatctctga
      181 acttatcacc gagcatggtc taatcaacct tgacttcgcc gacgtcaaag ctatcatggc
      241 caatgccggt tcggctctca tgggcatcgg ccgggcttcg ggtgacgacc gagccgctaa
      301 agctgctcaa caagctatcg agagcccgct aattgaagtt tcgattgacg gtgccaaggg
      361 agtactgttc aacgtcactg gtggctacga tatgtcgatg agcgagattc aagaagcggc
      421 tgaaatcatc accagcgccg tcgcaccgga tgccaacatc atctttggtg ccaccttaaa
      481 gccagaaatg gaagatgagc ttatcatcac cgtcattgct accggcttcg acagtgctta
      541 tttccacgag caagccgccg ccttggctga acgggatcgg ccggaagagg ttggtcagac
      601 cgaatcgact gagctagata agccagccga agttgaaaca gccgatgtcg actcgatcaa
      661 tatggactta gataacgaca tggccgacgc agtcaaagat gacttcgcca ccgagtctga
      721 gtcgactgct atttggcaga acgttgatga agaagaggct gacacaccag ctttcctacg
      781 tcgccgcaag aagaataaga aaaaagataa agacgacaaa taacaaaagc ccgggctgcc
      841 cccgggtttt taattggccc tagctacttc acaccgttta agctacacta ataattcaag
      901 cgacgactat cagttcaagc ataaacgcta tatgttgcac aaaatacact tgcaaggcgc
      961 tatatatagg gtttatagtc ttaagtagct acacgagggg gtgtagagag tcgtttacaa
     1021 ccaaggagac aatcaccgat gaaatgccct aaatgtcagc atccagattc aaaagttatc
     1081 gaatcccgcg aagccggaga cggtgactcg attcggcgac gccgtgaatg cttgaactgc
     1141 cattgtcgtt acactagcta cgagcgcgtg gagcgaccca acttggcagt cgtcaagaaa
     1201 aacggcgatc gtgaactgtt tgatcgtaat aagctgttcg gggccatcat ggtttcagtc
     1261 ggtaaattct tacgctccga tgtcgaagtc gagcagatcg tggctaaagt agaggatgaa
     1321 ctctatgctt taggtacgcc cgaggtgaca tcggcgcaga ttggcgacaa ggtgctagag
     1381 gaactcggca gccacaacgg cgtggcctat gtccgtttcg ccagcgtcta tcagaacttt
     1441 tcttcactgg aaggttttga gcgagccata aaacaagcgc gggaactaag ctccaaggaa
     1501 taaaacggcc aggtgccaaa ataaaaatca cctaaaaata aattaaaaaa actaaggagg
     1561 gtagctctat ggcacacacc gtgtcagcgc ttaacgtgga gcgctatttt accagcgacg
     1621 gggaacaccc gtacgatcaa ctaacttggg ctaagcgcga ttcgaagatc gtcaacccag
     1681 gtaccggcaa gacggtcttt gaacaaaaag acgttgagtt tccagaattc tggagccaaa
     1741 atgccatcaa catcgtgaca caaaaatact tttacggcac acctggtaca ccccagcgcg
     1801 aacagggcct aaaagattta atcgaccgcg ttgttgatac tataaccaaa cacggctacg
     1861 ataacggtta cttcgaatct aaaccagaga gcaagacttt ctctgacgag ctcaagtata
     1921 ttttgggtac tcagagggcg gcttttaaca gtccggtctg gttcaatatc ggtacaccgg
     1981 atcgctctca gcaggccagc gcttgtttta tcctagaagt cgatgacacc ctgccaagca
     2041 ttcttaactg gtatcgcgaa gagggcatga tctttaaggg tggctctgga gccggtctta
     2101 acctatcgaa gatccgttct tccaaggaga ccttgagttc ctctggcggt catgcttccg
     2161 gtccagtcag ctttatgcgt ggcgccgacg ccagtgccgg cactattcag tccggtggta
     2221 agactcgtcg agcagccaag atggtaatct tgaacatcga ccatccagac atcgaagagt
     2281 ttatctggtg caaagctcgt gaagaacgca aagccttcgt cttagagcaa gccggcttcg
     2341 agatgggcct aaacggtcgt gacatggtca gtatccaata ccaaaatgcc aataactcgg
     2401 tccgggtaaa cgacaagttc atgaaggccg ttgaaaataa tgatgactgg gaactggtcg
     2461 gtgtcaaaga caagaaagtc tatgatactg tcaaagctcg tgacctcttc cgccaggttg
     2521 ctgaagcagc ttgggaatcg gccgacccag gtatgcagtt cgacactact atcaacgatt
     2581 ggcacactac gccgaatgcc ggccggatca acggttctaa tccgtgctcg gagtacatgc
     2641 acctagataa ctcggcttgt aacttggcca gcatcaactt gctcaagttc ttgaggaacg
     2701 atggcacata tgacatagaa gcttacaagc acaccgtcga gacaatcttc ttagctcaag
     2761 aaatcctagt cggctacagc gaatacccaa ccgagaatat tggtaaaaac gccaaagcct
     2821 accgcgaact tggtatcggt tacgctaacc tgggcgccac tctgatggct aaaggcttgc
     2881 cgtacgactc cgacgaaggc cgggcttttg ccgctaccgt gaccgctatc ctgactggtc
     2941 acagctatgc taccagtgcc cgtatcgcta agcgtgtcgg tacctttgat ggttacgccg
     3001 atgacgagaa gggcatgata cgagtgctac gtaagcaccg cgatgccgta cgtgacattg
     3061 acgctagcct agtgcccgaa gaccttctca acgccgccgc aggagtttgg gatgacgcgg
     3121 tcgagctagg cgagaagtac ggcgtacgta actctcaggc
//
LOCUS       DASSBW010000060         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_879166, whole genome shotgun sequence.
ACCESSION   DASSBW010000060 DASSBW010000000
VERSION     DASSBW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879166"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFG56_01720"
     CDS             complement(<1..242)
                     /locus_tag="VFG56_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HET6622630.1"
                     /translation="MIVNSKDNHKLIETLQTGGVAVIKTDTLYGLVASANDRAAVARV
                     YELKRRNPSKSLIVLVADTDQLYDRPEQDLSDKWPGP"
     gene            262..1116
                     /locus_tag="VFG56_01725"
     CDS             262..1116
                     /locus_tag="VFG56_01725"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6622631.1"
                     /translation="MTSLEVVNNWFQTIGLHLLIVLIVAFAVRHVGGALIIYLINHTV
                     YNRKHGRHRLSEDDLVKRRDTLKNMLTVIWKVTVTIITVFVMIRTALPHVDLTPLFAS
                     AGIIGIALGFGAQSIIKDFLAGVFIITENQYRVGDYVEIDGLEGTVVRLTIRLTVLRA
                     FDGDVHYITNGDIQHVTNMTMEFGKVNFALKLDPATDIEKMIKTVNHVGEQITSEEAW
                     QDKITEAPMFYQITNITGSYIEIYVLGKTITAHQWDVEGELRRRLLDAFRKNNVKLAD
                     LPVSVIEK"
     gene            complement(1117..1488)
                     /locus_tag="VFG56_01730"
     CDS             complement(1117..1488)
                     /locus_tag="VFG56_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622632.1"
                     /translation="MRAAFLRNFTSQHKLLAAITGAIFVAMVLTVVSMSLYYSTGAEV
                     LDLSRPGYEAVRQAVNEKSHEDATFSGDGPVNQEVVEQFQGLYADSRKDLKSIDDFSN
                     QKPLSDDSLNLSPKSASSTGE"
     gene            complement(1493..2017)
                     /locus_tag="VFG56_01735"
     CDS             complement(1493..2017)
                     /locus_tag="VFG56_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622633.1"
                     /translation="MLKRISCLAFSLVFGVAASGLVNTTQVQALSDQQIDVIKSNCQS
                     SQVILKQIQLSDVATRISRGRDYQQLIKLMATFNSRLALNKIDGGQLTSITADMENQF
                     NDFQQTYITYSDQLSSLISLDCQDQPTEFYDQLNAARDLRLDLAKEVDTMDKLLDEYQ
                     QGVDDIAQQLSAES"
     gene            2080..2757
                     /locus_tag="VFG56_01740"
     CDS             2080..2757
                     /locus_tag="VFG56_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HET6622634.1"
                     /translation="MSRRWFLKLAIGLILVAATVLIHDWQNQAATPRLDQLSGDAATT
                     LAKLPVKGRAPKTDYSREQFGNGWETVGVCDVREQILRRDLTNIRLDSDRCTVLSGQL
                     LDPYTAQIILFKRGVTTSSDVQIDHIVALSDAWQKGAQGLSFSQRRAFANDPLELLAV
                     DGGANQQKSDGDAATWLPENKTFRCSYVARQIAVKFKYHLWVTAAERDAMTRVLNNCP
                     DQPLPSS"
     gene            2773..>3069
                     /gene="opgC"
                     /locus_tag="VFG56_01745"
     CDS             2773..>3069
                     /gene="opgC"
                     /locus_tag="VFG56_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HET6622635.1"
                     /translation="MAKTKFIPGRIAELDLLRGFFIFAIILDHAQHIPDIHMYLTGQG
                     KLWVSAAEGFFLISGLLTGYIRGFRQQDRPLWEQSKKLIKRGLLLYVWAIIITLL"
BASE COUNT          802 a          758 c          715 g          794 t
ORIGIN      
        1 ggaccaggcc acttatcgga caaatcctgt tccggtcgat cgtatagttg atcagtatcg
       61 gcgactagta ctattagcga cttagacgga ttgcgtcgct ttaactcata gactctggcc
      121 acggcagccc gatcattggc agaagctact aaaccataca gagtatcggt tttgatcacc
      181 gccacgccgc cagtttggag cgtttcgatt agcttatggt tatctttcga gttaactatc
      241 acaactgata tactagcacc aatgaccagc ctcgaagtag tcaacaactg gtttcagacc
      301 atcggccttc atctactgat tgtgctaatc gtcgcctttg ccgtacgtca cgtcggcggc
      361 gcactgatca tctacctcat caatcacacg gtctacaacc gtaaacacgg ccgccatcgc
      421 ctgagtgaag acgaccttgt taaacggcga gatacactca agaacatgtt gaccgtcatc
      481 tggaaagtaa cggtcaccat cataacggta ttcgttatga tccgaaccgc cctgccgcat
      541 gtagatttaa ccccgctgtt tgccagtgcc ggtatcatag gcatagcctt gggatttggc
      601 gctcagtcga ttatcaaaga tttcctagct ggtgttttta tcatcaccga aaaccagtat
      661 cgagtcggtg actacgtcga aatcgatggg ctggaaggca cggtggttcg gctaactata
      721 cgattaactg tcctacgggc gttcgatggt gacgtccact acatcaccaa tggcgatatc
      781 caacacgtga ccaacatgac gatggagttt ggcaaggtta actttgcctt gaaactagat
      841 ccggccaccg atatcgagaa aatgatcaaa acagtcaatc acgtcggcga gcaaatcacc
      901 agtgaagaag cctggcaaga taagattacc gaagcaccaa tgttctatca gattaccaat
      961 attaccggta gctatatcga gatttatgtc ctcggcaaga ctatcaccgc ccaccagtgg
     1021 gacgtagaag gcgaactacg ccggcgtctg ctcgacgctt tcaggaagaa taacgtcaag
     1081 ctggctgatc tgccagtgtc ggttatcgaa aaatagctac tcgccagtgc tactagctga
     1141 cttgggtgat aaattcaagc tatcgtcgct aagaggtttt tgattggaga agtcgtcgat
     1201 cgactttaga tccttacgac tatcggcata caagccttgg aattgttcta ccacttcttg
     1261 attaaccggc ccatcgccag aaaaggtcgc gtcctcgtgc gacttctcat taacggcttg
     1321 gcgaacagcc tcatagcctg gtctcgataa gtcgaggact tcagcacccg tcgaataata
     1381 aagcgacata ctgacgacag ttagcaccat tgctacgaat attgcaccgg tgatggcagc
     1441 tagtagctta tgttgtgaag tgaagttcct taagaatgcc gctctcatgg tactaggact
     1501 ccgcactcag ctgttgagcg atatcatcga cgccttgctg atactcgtcc aacagcttgt
     1561 ccatagtgtc gacctccttg gccaaatcta atctcaagtc acgcgccgcg ttgagctgat
     1621 cataaaattc ggtcggttga tcttggcaat ctaaactaat caagctacta agctgatcgc
     1681 tgtacgtgat gtaagtctgc tgaaaatcat taaactggtt ctccatgtca gcagtaatac
     1741 tggtcaactg gcccccgtca attttgttga gtgctaaacg ggagttaaaa gttgccatca
     1801 acttgataag ttgttgatag tcgcggccgc ggctaatcct ggttgcaaca tcgcttaatt
     1861 gaatctgttt taaaatgacc tgcgaagatt ggcagttgga cttgatgaca tctatttgct
     1921 gatcagataa agcttggact tgcgttgtat tgactaggcc gctagcagcc acaccaaaaa
     1981 ccaggctaaa cgccaagcag gatatgcgtt ttaacatatc tatattatcc cggtggactg
     2041 gctgagcgtc aaacgtcaaa taaactataa tgtataaaga tgtctagacg atggtttttg
     2101 aagctagcta ttggcctaat cttggtggct gctactgtct tgattcatga ctggcaaaac
     2161 caagccgcca cgccgcgatt agatcaactt agcggcgatg cagcaactac cctggcaaaa
     2221 ctaccggtca agggtcgagc gccaaaaacc gactatagtc gtgaacaatt cggtaatggt
     2281 tgggaaacgg ttggcgtctg tgatgtacgt gaacaaattt tacgccgcga tctgaccaat
     2341 atccggctag atagcgaccg atgcaccgta ctaagtggtc agttgctcga cccttacacc
     2401 gctcagataa tcttgtttaa acgcggcgtc actacttcaa gcgatgtcca gattgatcac
     2461 atagtagcgt tgtctgacgc ctggcaaaaa ggcgctcagg gcttgagctt cagccaacgg
     2521 agagcctttg ccaatgaccc tcttgagcta ctcgctgtcg atggtggcgc taaccagcaa
     2581 aaatcagatg gtgatgccgc aacctggttg ccggagaata aaacttttcg ctgcagctac
     2641 gtcgctcgac agattgccgt caagtttaaa taccacctat gggtaactgc cgccgagcga
     2701 gacgctatga cgagagttct aaacaactgc cccgaccagc ccctacctag ctcttagctt
     2761 ataatttata tgatggctaa gactaaattc ataccgggac ggattgctga gcttgatttg
     2821 ttaagaggct tcttcatctt tgccatcatc ttagatcacg ctcagcacat cccggacatc
     2881 catatgtact tgaccggtca aggtaaactg tgggtatcgg cggctgaagg tttcttttta
     2941 atctctgggc tgctcacagg ttatatccgt ggctttcgtc agcaggatcg ccctttgtgg
     3001 gaacaatcta aaaagctgat aaagcgcggc ttactgctat acgtgtgggc gattattatc
     3061 acactgctt
//
LOCUS       DASSBW010000061         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1573398, whole genome shotgun sequence.
ACCESSION   DASSBW010000061 DASSBW010000000
VERSION     DASSBW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573398"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFG56_01750"
     CDS             <1..239
                     /locus_tag="VFG56_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622636.1"
                     /translation="LLRERADMGILYGEDRITYDWAKQVLARRQVQATPPVAMVGYGY
                     FTLVPGQERFQLKLMAGVLGAILVLIAIANLFLS"
     gene            353..901
                     /locus_tag="VFG56_01755"
     CDS             353..901
                     /locus_tag="VFG56_01755"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET6622637.1"
                     /translation="MKIEFTAKLEKVGPFTIVRLPQTASDQLPSRGMAMVEGVINGQL
                     FQAPLEPDGQKSHWLKLTDLADKTQRAIGAKVGDTVTLTIAPSEQWPEPEIPDDLQAA
                     LDNDPEAKAVWDDTTPIARWDWIRSVRSTRNPETRARRIEVACSKLRSGKPRQCCFNR
                     SECTEPAVSKGGVLVDSEVTAG"
     gene            898..1359
                     /locus_tag="VFG56_01760"
     CDS             898..1359
                     /locus_tag="VFG56_01760"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6622638.1"
                     /translation="MKIQNMEQGREGWRPSRQEVDDFLREQTLCTIASLDADGHPQVA
                     TVAFSVTEDHKLIIGTNESSRKAGNIARDPHVAVVVTDEDQRYTVQLEGTAQILSPEE
                     FEPYSDEHYNQLPASRPFKDQPGEVNILVGPTHLRFSDCDVHPWQKTEYDY"
     gene            1402..1989
                     /locus_tag="VFG56_01765"
     CDS             1402..1989
                     /locus_tag="VFG56_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622639.1"
                     /translation="MSVEHLNPGSVEGYDDFETAAFGLQSDLQAELDNLVETGQPLMD
                     EDFEQKSLTLLKLTDSLGRLFFHEFDVDMDEAIATSHCAFYFGLLLGRQLNPGKEWGF
                     DLSELQQFDDPLEFKEFIERRADSYLQYRKTIDGLIGRYMPVISPNPQLEAGAETIVA
                     FALAQVELEHFDNFVTAEIADSASYLDDLETLTEE"
     gene            2061..2291
                     /locus_tag="VFG56_01770"
     CDS             2061..2291
                     /locus_tag="VFG56_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622640.1"
                     /translation="MNEDTQNNQHNAPKIKTTKVVYPQIYSYILPDEAENEGSQKIGY
                     TERKDVDKRIYEQTHTAAKHLRFEKRWSAPAF"
BASE COUNT          591 a          674 c          641 g          496 t
ORIGIN      
        1 agctgctgcg tgagcgcgcc gacatgggta tcttgtacgg cgaagaccgg atcacttacg
       61 actgggccaa gcaggtactg gctcggcgcc aagttcaggc gactccgcct gtggctatgg
      121 tcggctacgg ttacttcacg cttgtaccgg gccaagaacg atttcagctc aagctgatgg
      181 ccggcgtgct cggagcaatc ttggttctca tcgccatcgc caatctcttc ctttcctaaa
      241 ccgggagacc cgcactgacc tgagcgtttt gatgcattag cccgacgctt ggatacataa
      301 ggtgcgggct tttgcatgcc ttggtataat caaaaggtag gaagaattac tcatgaagat
      361 cgaatttaca gctaagcttg agaaagtcgg accgttcacc atcgtccggc tgccgcaaac
      421 tgccagcgac cagctaccct cgcgcggcat ggcgatggta gagggcgtca tcaacggtca
      481 gttattccag gcgccgctgg agccggacgg ccaaaagagt cattggctaa agttaaccga
      541 cctggccgat aagacccagc gggccatcgg cgccaaggtg ggcgatacgg taacgctgac
      601 catcgcgccc agtgaacaat ggcccgagcc ggagatcccg gacgacctgc aagccgcgct
      661 cgataacgac cccgaggcca aagccgtctg ggacgacacc acgccgattg cccgctggga
      721 ctggatccgc tcggtccgct cgactcgcaa ccctgagacc agggctcgcc gcatcgaggt
      781 ggcgtgctct aagctacgtt ccggcaaacc acgccagtgc tgcttcaacc ggtccgaatg
      841 caccgaacca gccgtctcca aaggcggcgt actggtcgat tcagaggtca ctgccgggtg
      901 aagattcaga acatggagca gggccgcgaa ggctggcgac cgagtcggca agaagtcgat
      961 gactttttgc gcgagcagac gctctgcacc atcgccagtc tcgacgccga tggtcaccca
     1021 caagttgcta cggtagcttt ttcggtgacc gaagatcaca agctgatcat cggcaccaac
     1081 gagagttcgc gtaaagccgg caacatagcc cgcgacccgc acgtcgccgt ggtcgtcact
     1141 gacgaagacc agcgctatac cgttcaactt gagggcacag cccagatcct cagcccggaa
     1201 gagttcgagc catactccga cgagcactac aaccaactgc cggcttcgcg accgtttaag
     1261 gaccagccgg gtgaggtcaa tatcttggtc ggcccgacac atctgcgctt tagcgactgc
     1321 gatgtccatc cttggcaaaa gaccgaatac gactactaga ttggcctaat tccggctact
     1381 ggtgtatttt agggataagt tatgagcgtg gaacacctaa accccggcag cgtggaaggt
     1441 tatgacgatt tcgagacagc agctttcggc ttgcagtccg acttgcaggc ggagctcgac
     1501 aatctagtcg agaccggcca gccgctgatg gacgaagact ttgagcagaa gagtctgact
     1561 ctgcttaaat taaccgactc gctcggtcgg ctgttcttcc atgagtttga cgtcgatatg
     1621 gacgaggcta tcgccacctc tcattgcgcc ttctatttcg gcctactgct tggccgtcag
     1681 ctcaaccccg gtaaggagtg gggcttcgac ctgtcggagc tgcagcagtt tgacgatccg
     1741 cttgagttca aggagtttat cgagcgccgc gccgacagtt acttgcaata ccgcaaaacc
     1801 atcgatggtc tgattggccg ctacatgcca gtcatcagtc ccaacccgca gttagaagcc
     1861 ggcgctgaaa ctatcgtcgc cttcgccctg gctcaagtcg aactcgaaca cttcgacaac
     1921 ttcgtcaccg ccgaaatcgc cgacagtgct agttatcttg atgatcttga gactttgacc
     1981 gaagagtaaa ttatatataa gtgttactgt ataaaaacac aaaatagcat tttctcaaga
     2041 acagaggtag tatagaggtt atgaacgagg acactcagaa caatcaacat aacgcaccaa
     2101 aaattaaaac cacaaaagtt gtttatccgc agatttattc ttatattctt cctgatgaag
     2161 cagagaatga aggctcacaa aaaatcggct acaccgaacg caaggatgta gacaagcgaa
     2221 tttatgagca aacccatact gccgcgaagc atcttaggtt tgagaagcga tggagtgcgc
     2281 cagcatttta gccatagtgg ttcagaatga tacgcataac ggtttgacca gcaacgaaag
     2341 agcccggcgc tcgttgtaag cgccgggcaa ccgttctcga ccagcgagtc gaatcctact
     2401 cg
//
LOCUS       DASSBW010000062         9504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1337522, whole genome shotgun sequence.
ACCESSION   DASSBW010000062 DASSBW010000000
VERSION     DASSBW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337522"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /gene="dnaG"
                     /locus_tag="VFG56_01775"
     CDS             <1..1597
                     /gene="dnaG"
                     /locus_tag="VFG56_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET6622641.1"
                     /translation="FMVSPDKQIWHDFSSNRGGDVFSFIMEVEGLDFRQALEMLARQA
                     GVDLSRYDKPGAKDISSKKNRLLKLLDLAATYYQRQLISNPTAIDYVSKKRSLNRQTV
                     LDFRIGYSPSSGLISFLKSKSYNDRDIKDAGLINSRGQEMFRDRMMVPLSDGQGQIVG
                     FTARLIRDVEGAPKYINTPATLLYDKSRQVFGLAQAKQAIRKAGYVVIVEGNLDVVSS
                     HQAGVKQVVATAGTAMTEQHLKSLKRLAGDIRLAFDADSAGLAAAERAIGLAQNVGLS
                     LSMVVLPSGSKDPDELIKADVSAWEKVIDKPQPAIDWLIDRYSLETDITTAAGKQIVS
                     TKALEIVAKLNDPVEREHYLQMLSGQIGISLKSLEERLDQLKTPAGRLKTVKVDHIEP
                     ESYAHEDYLLALLTVEPTAQDIARKLEVEDFHGEERRMLFRYLRDDPKPFKADKMPKT
                     LHEIETYVKLVLFKAENRYDDLSGQDRLVEAVSLVKRLKAQHLKERKQTLIGALKRAE
                     ADGDETTIDALNIELNKLIREES"
     gene            1597..2613
                     /gene="rpoD"
                     /locus_tag="VFG56_01780"
     CDS             1597..2613
                     /gene="rpoD"
                     /locus_tag="VFG56_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET6622642.1"
                     /translation="MPRKKATPKKDDDDLKLNDDVQQSDEREEGMAGLDEVEPDTEEL
                     ADEEEVDTSQPVYFDDVSDDSVRLYLREIGKIPLLSAEEELALAQRVVAGDKKAKDKM
                     AEANMRLVVSIAKRYSGRGLDFLDLIQEGNTGLLRAVEKFDPDKGFKFSTYATWWIRQ
                     AITRAIADQARTIRIPVHMVETINKLLRTQRRMTQELNREPTVEELGKELEMEPEKVE
                     YVMKIKQEIHSLDAGVGREDGDDDSTLSDFIEDEDATSPEDSAAGQLLKEQVQGVLGT
                     LSEREQKILRMRFGLDDGKNHTLEEVGQEFAVTRERIRQIEAKALAKLRKHKDAKKLY
                     EYLQ"
     gene            complement(2896..3462)
                     /locus_tag="VFG56_01785"
     CDS             complement(2896..3462)
                     /locus_tag="VFG56_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6622643.1"
                     /translation="MTKPKIIAIVGMPGSGKGTTVDHLEDSYHLPKVYFGGMVYDEVK
                     RRGLDIVMDERMVREDMRKQEGPAVLAKRAALKADEMLDDGAQAVVLDGLYSWSEDRY
                     LREKYDDSIITIAIVNPKHIRYERVVARKDAHRPYTLEQIQLRDIEEIENIEKGGPIA
                     FADYYLANAKNVDELKVQIDELMQQLGF"
     gene            3532..3807
                     /locus_tag="VFG56_01790"
     CDS             3532..3807
                     /locus_tag="VFG56_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET6622644.1"
                     /translation="MEQPLPPQLWPEEGECFRQADPELFFPVGEKTTSDKIAIEEAKS
                     YCRRCTMADYCLKWALANEDHGIWGGMTANERQELKRQQKRQKSRAA"
     gene            3830..6400
                     /gene="polA"
                     /locus_tag="VFG56_01795"
     CDS             3830..6400
                     /gene="polA"
                     /locus_tag="VFG56_01795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET6622645.1"
                     /translation="MTKRLVIIDGKSVFYRGYYAMPNLATKDGRPTGGVYGFAALSLE
                     VMKRLKPDYVVVAWDKKGTNIRKRKEIYPDYKAGRKPAPPDFYEQIPMLYELLEAFSW
                     PLIELDDYEADDIMGTLAVQAADQQIETCLVTSDLDVLQCIGPLTHVYAMKTGFTNIE
                     MFNPNTFKNKYGIDVHQFLDLKALKGDSSDNIPGVAGVGEKTALDLLQNYQTLDGIYE
                     HLGDIKDATRKKLEAGRDSAYMSKKVAEIWCDAPVKLDLKDSDVKDLDKPKVTSLLKQ
                     LQFTSLLTKLPDFMTATEEIPDDSAELPPTELVDLDEQARVALTMAPTVMAAELDEDR
                     WLVSDKVGTAYVIDVTKQAELISGLNLVAYDLKPLFKRLLEAGHQSLPQPIHDVRQAA
                     FLLDPLSKYRQLSDLAGAKVDTAEQQISALHKVYASQAAQLDDLPKLADLARNIDFKL
                     IPILSRMESIGIKLDTKQLADMDQELSKKLASVDRQMFDMVGYEFNIGSPSQLAEVLY
                     GKLQLSTVGIKKGKTGYSTGQKELDKLRGSHPIVELIEQHRELSKLQNTYVQSLPKLV
                     DDQSRLHTTFNQDVAATGRLSSTDPNLQNIPIRSEAGRKIREAFVPEQGHVFVSADYS
                     QFELRLAAVLAKDKQMVDDFNSDIDIHAKTASDVYGVALGQVTKNQRRDAKVINFGVL
                     YGMGPHALAQNTGMSFAEAKHFIDEYFRLRAPIRKYIDATIEGAKTHGYVETLFGRRR
                     PSPDVSSSNFVVREAAKRAAANMPIQGTEADLMKMAMIEVDKKLNDAFTEGSSEETKL
                     HGLASQILQIHDSILVECAETDATMVGEILKDTMENIYPDLGVKLKVDVSSGDNWGLL
                     "
     gene            6493..7011
                     /locus_tag="VFG56_01800"
     CDS             6493..7011
                     /locus_tag="VFG56_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET6622646.1"
                     /translation="MARVAILVDNYFEQIEMTSPKQALEEAGHETTLVTTREDEVMGL
                     NHIEKADKFEADELLDEVSFDDYDALVIPGGVVNADALRMDQKARDWVKHCFDNNKPL
                     AVICHGPWLLVSSGLVDGHKLTSWPTLEDDIKNAGGDWVDQDLVEDGVLITSRKPDDL
                     PVFNQALLDHLG"
     gene            7052..7486
                     /gene="dut"
                     /locus_tag="VFG56_01805"
     CDS             7052..7486
                     /gene="dut"
                     /locus_tag="VFG56_01805"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET6622647.1"
                     /translation="MNIQIKRLSPRAQLPEYQTTGSAAVDLQACLDKPVTLKPLERAV
                     ISTGLALSLPEGVHAEIRARSGLSLKHGITLANGVGTIDADYRGEIGIILMNLSNQTF
                     VVEDGMRVAQMLLVSYETFDWHEVDVLDVTERGSGGYGSTRH"
     gene            7467..8069
                     /locus_tag="VFG56_01810"
     CDS             7467..8069
                     /locus_tag="VFG56_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6622648.1"
                     /translation="MAQPDTRRSMELKAIIFDCFGVLQVDYTHAFYEEHVKDYERLKP
                     QLLELNRQHDYGFISQTEFVQAVADLARLSTSFVTEHMDSKLVRNQPLVDYMAELKAN
                     YRLGMISNIGPDTMERYFARDELGQIFDAVVLSGEETIAKPHPYIYQLAAERLGVPVG
                     ACVMVDDSEENCAGADAAGMKSIYYQGFKSFKRQLIQLTN"
     gene            8129..9025
                     /locus_tag="VFG56_01815"
     CDS             8129..9025
                     /locus_tag="VFG56_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6622649.1"
                     /translation="MCKVTEHPITHREHKHSVVMTESIHHRRGGHVLMATALPKRPEF
                     DKAPVLICNGYGAGIGAMGRLATVLAEQEGRAVITYDELRSRAAWADPVSFRSETILS
                     VMDRFDKDAGDYFSRPTLVGWSTGGPAAIGVAEYLLKNDEQERLGAVIPTASAGIGQQ
                     ASEIELTVRAGLEIARGFRSPKVICALGAKAAQGFAGYLCRDIILSAREVGALSNLNM
                     SERLADVDRAGVPLRIIAMTEDIIFPINKMTAAVEQIGLGHKLRSINGSHINLAWQPR
                     VIKDVVNVIEEAEAVNPTTSAA"
     gene            9090..9320
                     /gene="tatA"
                     /locus_tag="VFG56_01820"
     CDS             9090..9320
                     /gene="tatA"
                     /locus_tag="VFG56_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6622650.1"
                     /translation="MRTKTMVSSQVAEILGLGTPELIVILVILVLLFGAKKLPELARS
                     IGTSANELKTSMNGGKKDDAKSAGKNVDGANQ"
     gene            9298..>9504
                     /locus_tag="VFG56_01825"
     CDS             9298..>9504
                     /locus_tag="VFG56_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET6622651.1"
                     /translation="MSTEPTNNQTVREELRTFGDHIGELRKRVVWVALVFVVASALAY
                     NFRDILIDIVLKPLGNQKLVYLTPA"
BASE COUNT         2559 a         2187 c         2489 g         2269 t
ORIGIN      
        1 ttttatggtg agtccggaca aacagatctg gcacgatttt tcgtcgaacc gcggcggtga
       61 cgtcttctca tttatcatgg aagtcgaagg tctggacttc cggcaggcac tcgagatgtt
      121 ggctcgccag gctggcgtgg acttaagtcg ctacgacaaa ccgggcgcca aagatatctc
      181 aagcaagaaa aaccgattac taaaattact cgatttggca gcgacctact atcagcgcca
      241 gctgatcagt aacccgacag ccatagatta tgtcagcaaa aaacggagtt tgaatcgcca
      301 gactgtactt gattttcgta ttggttactc tccaagcagc ggactcatca gctttttaaa
      361 gtccaaaagt tataacgacc gggatatcaa ggatgccggt ctgatcaatt cacgcggaca
      421 agaaatgttc agagaccgga tgatggtacc tctatctgac ggccaaggtc aaatagtcgg
      481 ctttaccgct cggttgattc gcgatgtaga aggtgcgcct aagtacatca atacaccagc
      541 tacgctgctt tatgacaaga gtcgccaggt cttcggcttg gcacaggcca aacaagcgat
      601 tagaaaagcc ggttatgtcg ttatcgttga aggtaatctc gatgttgtca gtagccacca
      661 ggccggggtt aagcaagttg tagccaccgc cggtacggct atgaccgaac aacacttgaa
      721 gtcactcaag cggttggctg gtgacataag actagccttt gatgccgata gtgctggtct
      781 tgctgctgcc gaacgggcga ttggcttggc acaaaacgtc ggcttaagcc tcagtatggt
      841 ggtgctgccg agtggctcga aagatccgga tgaactaatc aaggccgatg tcagcgcttg
      901 ggaaaaagtc atcgataaac cgcagccagc tattgactgg ttgattgatc gctattcgct
      961 tgaaaccgat atcaccacgg cagccggcaa gcagatcgta tcgaccaagg cgctagagat
     1021 agtcgctaag cttaatgacc cggtggagcg tgagcactac cttcagatgc tatctggaca
     1081 aatcggcatt tcacttaagt cgctcgaaga acggctggat cagctcaaga ctcctgccgg
     1141 caggctgaaa accgtcaaag tcgatcatat cgagccggaa agttacgccc atgaggacta
     1201 tttgttggcg ctattaacgg ttgaaccgac agctcaggac attgctcgta aactcgaagt
     1261 cgaagacttt cacggcgagg agaggcgaat gttatttcgc tacttgcgag acgatcccaa
     1321 gcccttcaaa gcagataaga tgcccaagac tttgcatgag atagagactt atgtcaaact
     1381 agtattattc aaagccgaga atcgctatga tgacttgtcc ggacaagacc ggttagtcga
     1441 agcggtcagc ctcgtgaagc gactaaaagc ccaacaccta aaagaaagaa aacagacact
     1501 gataggcgcg ctaaagcgtg ctgaagccga tggtgatgaa acgacgatcg atgctttaaa
     1561 tatagagctt aataaactaa taagggagga aagttaatgc ctcgtaaaaa agctacaccc
     1621 aaaaaagacg atgacgattt gaaattgaac gacgacgttc agcagtcgga tgaacgtgaa
     1681 gagggcatgg ccggcctgga tgaagtcgag ccggacaccg aggaactcgc cgatgaagaa
     1741 gaagtcgaca cctctcaacc ggtctatttt gatgacgtat cggacgactc agttcgttta
     1801 tacttacgtg aaatcggcaa aatcccgctt ttaagcgccg aagaagaatt ggcactggct
     1861 caaagagtcg tggctggtga caaaaaagcc aaggacaaga tggctgaagc caacatgcgt
     1921 ttagttgttt cgatagctaa gcgttactct ggtcgtggtt tggacttctt ggacctaatc
     1981 caagagggta ataccggttt gctgcgtgcc gtcgagaaat tcgacccgga caagggcttt
     2041 aaattttcaa cttacgctac atggtggatc cgtcaggcta tcactcgggc tatcgccgat
     2101 caggctagaa ccatccgtat cccagtccac atggtagaga ctatcaacaa gttgcttagg
     2161 actcagcgtc gaatgaccca ggagttaaac cgcgagccga ctgtcgaaga gcttggtaag
     2221 gaacttgaga tggaaccgga aaaggtagag tatgtcatga aaatcaagca ggaaatccac
     2281 tcacttgacg ccggtgtcgg ccgggaagac ggtgacgatg attcgacttt gtcagatttc
     2341 atcgaggatg aagacgctac tagcccagaa gattcagccg ccggccagct actaaaagag
     2401 caggttcagg gtgtgcttgg tacgctaagc gagcgcgaac agaaaatctt gcgcatgcgg
     2461 ttcggtctag atgacgggaa gaaccatact ctagaagagg tcggtcagga attcgccgtc
     2521 acccgtgagc gaatccgtca gatcgaagcc aaagctctag ctaaactgcg caaacacaaa
     2581 gacgccaaga agctatacga atatctgcag taggcaagtt acgctcagcg tcgtcatttt
     2641 ccatcttgcc ggcgcttgcg cttgccgcct caccaccatc tttctcgcat ccagttgtct
     2701 tcacgccatg tcgtactagc aatacgtcgc tcgatttatc tgtgcgcgga gaacaagctc
     2761 gtgctcgata attcgggcgt cgattactag tactcttggg tttgtcaaag gatatgagaa
     2821 agagatggtg ggtggcatcg ccgctagcga ctactcggtc tcgccagcgc gaagcggaaa
     2881 tggtcgggat aataactaga agcctagttg ttgcattagc tcatcgattt ggacttttaa
     2941 ctcgtctacg tttttggcgt tggccaagta gtaatcagcg aaagcgatgg ggccgccttt
     3001 ttcgatgttt tcgatctctt caatgtcacg cagctggatt tgctccaatg tatatggacg
     3061 gtgagcgtct ttacgggcga ctacccgttc atagcgaatg tgttttgggt tgacgatggc
     3121 gatggtgatg atactatcgt cgtatttctc gcgtaggtag cgatcttcgc tccacgaata
     3181 aagcccatct agaactaccg cttgggcgcc atcgtccaac atctcatcag ccttgagagc
     3241 agcccgttta gccagtaccg ctgggccttc ttgttttcgc atatcttcac gaaccatcct
     3301 ctcatccatg acgatatcga ggccacgtct tttaacttcg tcgtagacca taccaccgaa
     3361 gtaaactttg ggcagatggt agctatcctc taagtgatca acagtagtac ccttgccgga
     3421 accaggcata ccgacgatag cgataatttt cggtttagtc atcactaaac agtataacaa
     3481 gactagcgct taagcttgtc gctaaaagca tcggtcttca taattgctta catggaacaa
     3541 cccttgccac cccagctatg gccggaagaa ggcgaatgtt ttcgtcaggc cgatccggaa
     3601 ttgttttttc cggttggtga aaagactaca agtgacaaga ttgctataga agaagccaag
     3661 agctactgtc gccgatgtac gatggccgac tattgcctta agtgggcgtt agccaatgaa
     3721 gatcatggta tctggggtgg catgacggcc aacgagaggc aggaactaaa gcgtcaacaa
     3781 aagcgacaaa agtcccgagc cgcttgagcg actccgttat actagagtta tgacaaagcg
     3841 gttggtgatc atcgatggca agagtgtgtt ttatcgtggt tactatgcca tgcctaattt
     3901 agctaccaaa gatggccgac caacgggagg tgtttatgga tttgctgcgc taagcttgga
     3961 agttatgaag cgcttgaaac cggattatgt agtggtagcc tgggacaaaa aaggcaccaa
     4021 tatacgtaag cgcaaggaga tctatccgga ctataaggct ggacgtaagc cggcaccgcc
     4081 ggatttctac gagcagattc cgatgctata tgagttactc gaagcttttt cttggccgct
     4141 tatcgagctt gatgactacg aagccgacga tatcatgggg acactggccg tacaggcggc
     4201 ggatcagcag attgaaactt gcctagtcac ttcggatctt gatgtactgc agtgtatcgg
     4261 gccgcttact catgtctacg ccatgaagac tggctttacc aacatcgaga tgtttaatcc
     4321 caataccttt aagaataaat acggcatcga cgtacaccag tttttggacc taaaagccct
     4381 caagggcgac tcgtcagaca atataccggg tgtagccgga gtcggcgaaa agacggcgct
     4441 tgatttgctg caaaattatc agacactgga tgggatttat gaacacctgg gcgatatcaa
     4501 agatgctact cgcaagaaat tagaagccgg tcgagactcg gcttatatga gcaaaaaagt
     4561 cgccgaaata tggtgtgatg caccggtcaa gttggatcta aaagattctg atgtcaaaga
     4621 tctcgataag ccaaaagtta cctctctttt aaaacagtta caattcacca gcttgctgac
     4681 taaactgccg gactttatga cagcgaccga agagataccc gatgattcgg ctgaattacc
     4741 accgactgag ctagtggatc tggacgagca agccagagtg gctctgacga tggcgccgac
     4801 cgttatggcg gccgaactag atgaagaccg ttggctggta tctgacaaag ttggcacagc
     4861 ttacgtaatc gacgtgacca aacaggccga gttaatcagc ggtctgaacc tggtggctta
     4921 cgatttgaaa ccattgttta agcgcttgtt ggaggctggt caccagagcc taccgcaacc
     4981 gattcatgat gttcgccaag cagctttctt gcttgatcca ctcagtaaat atcgacaact
     5041 tagcgatcta gctggcgcca aagtggacac agctgagcag caaatcagcg ctctacataa
     5101 ggtctatgcc agtcaagccg ctcaacttga tgatttaccc aagctggcag acttggcgcg
     5161 caacattgac tttaagctga tacccatcct gtcacggatg gaatctatcg gtatcaagct
     5221 cgacactaag cagctggctg acatggatca agaattgagc aaaaaattag ctagtgtcga
     5281 ccggcagatg ttcgatatgg tcggctatga gttcaatatc ggttcaccca gccaactagc
     5341 cgaagtcctt tatggcaagc tgcaactgtc gactgtcggt ataaaaaagg gtaaaaccgg
     5401 atattcaaca gggcaaaaag agctcgataa gttacggggt agccatccta ttgtcgagtt
     5461 gatcgaacag caccgtgaat tgtccaaact gcagaacact tatgtccaaa gcttgcccaa
     5521 actagtcgac gatcaatcac gtctgcacac tacctttaat caggacgtag cggcgacagg
     5581 taggttgtct tcgactgatc cgaatttgca gaacatacca atcaggagtg aagctgggcg
     5641 gaagattcgc gaagctttcg tgccggagca aggccatgtt tttgtcagcg ccgactacag
     5701 ccagtttgag cttcgactag ctgccgtgct tgctaaagat aagcagatgg tagatgactt
     5761 taactctgac atcgacatcc atgctaagac cgccagcgac gtctatggtg tggcgcttgg
     5821 gcaggtcacc aagaaccaac gtcgagatgc caaggtgatc aactttggtg tgctttacgg
     5881 catgggtcca catgctttag cgcaaaacac cggtatgagc tttgctgaag ctaagcattt
     5941 catcgacgag tatttccgat taagagcacc gattcgcaag tatatagacg cgacgatcga
     6001 gggagccaaa actcatggct acgtcgagac actatttggt cggaggcgac cttcaccaga
     6061 tgtaagttcg agtaattttg ttgttcgaga agccgccaag cgagccgccg ccaatatgcc
     6121 gatccaaggt acagaagctg atttgatgaa gatggcaatg attgaagtag acaagaagtt
     6181 gaacgacgct ttcaccgaag gatcttccga agagaccaag cttcacgggc tagccagtca
     6241 gatattacaa attcatgact cgatattggt agagtgtgcc gagaccgatg caaccatggt
     6301 tggtgagatt cttaaagata ccatggaaaa catctatcca gatctcggtg ttaaactgaa
     6361 ggtcgatgtc agcagtgggg ataactgggg cctactataa aaacaataac tggtgcttgt
     6421 ggtatttttc actgctcgcg gtctagctcg gctctaacct aagactgtga cctaaaaagg
     6481 aagggggcgt ttatggcgcg cgtggcgata ctagttgata attactttga gcaaattgag
     6541 atgaccagtc ctaagcaagc tttagaggaa gccggtcacg aaactacact agtgacgacc
     6601 cgagaagacg aggtaatggg tctcaaccat atagagaagg ctgataagtt tgaagccgat
     6661 gagttattgg atgaagtaag ctttgatgat tatgatgccc tagtgatacc gggtggagta
     6721 gtcaatgccg atgcccttcg gatggatcaa aaagctagag attgggtcaa gcactgtttt
     6781 gacaataaca agccattggc tgtcatctgc catggtccat ggctattggt atcaagcggt
     6841 ttggttgatg gtcataaatt gactagctgg ccgacgctgg aagatgacat caaaaacgcc
     6901 ggaggtgact gggttgacca agacttagtc gaagacggtg tgcttataac atctagaaaa
     6961 cccgatgatt tgccggtctt caaccaagcc ctgctcgacc acctaggctg atggttgacc
     7021 ccgactgaat cgactatgtc acaatagagg catgaatatc caaatcaaac ggctcagtcc
     7081 tagggctcag ctaccggagt accagactac tggtagtgcg gcggtggatc ttcaggcttg
     7141 tctggataaa ccagtaactc tgaagccact agagcgagcg gttatatcga ccggcctggc
     7201 gcttagcttg ccggaaggcg ttcatgccga gatccgggct cgcagtggtt tgagcttgaa
     7261 acatggcatc accttggcta acggcgttgg caccatcgac gctgattatc gtggtgaaat
     7321 cggcataatc ctgatgaatc tgtcgaacca gacatttgtg gttgaagatg gtatgcgcgt
     7381 ggcacagatg ctactggtta gctatgaaac ctttgattgg catgaagtcg atgtgctaga
     7441 cgtaaccgag cgtggtagtg gcggttatgg ctcaacccga cactagaagg tctatggagc
     7501 tcaaggctat tatcttcgat tgctttggcg tattgcaggt tgattacacc catgcctttt
     7561 atgaagagca cgtcaaagat tatgagaggc taaagccgca gctcttggag cttaatcgcc
     7621 agcatgatta tggcttcatc agccaaactg agtttgtcca agccgtggct gacttggcca
     7681 ggctgtcgac ttcgtttgtg acagaacata tggacagtaa actggtgcgc aatcagccac
     7741 tggttgatta tatggccgag cttaaggcta actataggct tggtatgatc agtaacattg
     7801 gcccggatac gatggagcgc tactttgccc gcgatgagct aggtcagata tttgacgcag
     7861 tggtgctatc gggtgaggaa actatagcta agccgcaccc ttacatctat cagctggcag
     7921 ccgaacgtct gggtgtaccg gtcggcgctt gtgtcatggt cgacgacagt gaagagaatt
     7981 gtgctggcgc cgatgcggct ggcatgaaat ctatttatta tcagggcttt aagtcgttca
     8041 agcgacaact aattcagctg actaattaag ttaaatcttc ttgtactaga ctgcccgacg
     8101 gcgtacagtg ctttaaaagg cattgtagat gtgtaaggtg acagaacacc cgataaccca
     8161 ccgggaacac aagcactcag tcgttatgac ggagtcaatc catcaccgcc gaggtggaca
     8221 cgttttgatg gcgacagccc tgccgaaacg accggagttc gataaggctc cggttttaat
     8281 ctgtaatggc tatggcgccg gtataggtgc tatggggcgt ttggctaccg tattagccga
     8341 gcaagaaggt cgagcggtaa taacatatga cgagctaaga agcagggcgg cgtgggctga
     8401 tccggtcagc tttcgttccg agactatatt gagcgtcatg gatcgctttg acaaggatgc
     8461 cggagactat ttctcccgcc ccactttagt cggctggtcg actggcgggc cggcagctat
     8521 cggagtagct gagtatctac tcaagaatga tgaacaagag cgcttaggag cggttatccc
     8581 aaccgcttcg gctggcatcg gccagcaagc tagcgaaatc gagttaactg tcagggctgg
     8641 cctggaaatt gctcgaggct ttaggtcgcc caaggtaatc tgtgcgctcg gcgccaaggc
     8701 tgctcaaggc ttcgccggtt atctttgccg tgacatcatc ttgtcggcta gagaggttgg
     8761 agcgttatcc aatcttaata tgagtgagcg tttagccgat gtcgacagag ctggcgtgcc
     8821 gcttagaatt atcgctatga ctgaggatat aatttttcca atcaacaaga tgacggcagc
     8881 ggttgaacaa atcggcctag gtcataagct caggtcaatt aatggtagtc acatcaactt
     8941 agcttggcag ccacgggtta tcaaagatgt ggtcaatgtc atcgaagaag ccgaagcggt
     9001 gaatccgact acttctgccg cctgaaggct agcataaccg ctattgattt tagccgatat
     9061 aaccgctaat ctaatagtta gaaaactaag tgaggacaaa aacaatggta agtagtcaag
     9121 tagctgaaat cctaggccta ggcacaccag aactaatcgt tatcctggtt atcctagtct
     9181 tgcttttcgg tgccaagaag cttccggaac tggctcgttc tatcggtact tcggccaatg
     9241 agcttaagac cagcatgaac ggcggtaaga aggacgacgc aaagtcggca ggtaagaatg
     9301 tcgacggagc caaccaataa tcaaactgtc cgcgaagagc taaggacgtt tggcgaccac
     9361 atcggcgagc ttaggaagcg cgtcgtatgg gtcgctttgg tttttgtagt agcttcggcc
     9421 ctcgcttata acttccgtga catactcatc gatatagtcc taaagccact tggtaaccaa
     9481 aagttggttt acctaacgcc ggcc
//
LOCUS       DASSBW010000063         6833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_134238, whole genome shotgun sequence.
ACCESSION   DASSBW010000063 DASSBW010000000
VERSION     DASSBW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134238"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFG56_01830"
     CDS             complement(<1..220)
                     /locus_tag="VFG56_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622652.1"
                     /translation="MKAKEIDRLPIKPDIPPSSRKIRRFAPKYLESIIASRVMPGVKS
                     IDELRPEHIEALSGGNDAAIYLVNSPEHN"
     gene            321..1901
                     /gene="treF"
                     /locus_tag="VFG56_01835"
     CDS             321..1901
                     /gene="treF"
                     /locus_tag="VFG56_01835"
                     /inference="COORDINATES: protein motif:HMM:NF009773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HET6622653.1"
                     /translation="MPDKNPIRKTFNQLKATVKITQLTVQPVSKSPDRVLGELFQAVQ
                     LSRLHPDGKTFVDQVPSARMRQVVRRYRKEADRPDFDLYEFVAKNFADFTPSSQSVKA
                     EPSTTAEDHIIRLWPHLTKTAYKSIGSLIALPNPYVVPGGRFQEQFYWDAYFIMLGLE
                     VSGRSDLTDCMMSNYSHMFRKLGFIPTGNRTYFTSRSQPPFYSLMIDLMIRRKGKKYL
                     TTRLPYLLQEYHFWNKHSRLPLKPKRRVKLDKNTYLNRYYDNYPSPRPESYSEDVETA
                     KLAKDRTDKQVYLDLKAGAESGWDFSSRWFDDPMKIETIRTTNLIPVDLNCLLYKLEL
                     TIADCYRTLLQFPLAKYYRDRATKRKAAINHYLWDESKGFFFDYDFVNHKKSEDYTLA
                     GVFALYVGLADDNQAAKVAGVIEDKFLKDGGVVTTLQDTGQQWDWPNGWAPLQWTTIV
                     GLRRYGYHELADAIKFRWLATNEALYLNEGKFVEKYNVVDPTERGGGGEYQLQDGFGW
                     TNGVFLALKKDFDVELSA"
     gene            complement(1902..3023)
                     /locus_tag="VFG56_01840"
     CDS             complement(1902..3023)
                     /locus_tag="VFG56_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6622654.1"
                     /translation="MKLPKSLYTVLVFYKINTLRFFRDKLSIFFGIGFPLIFLFVFGG
                     LNSGGGDISFNVALINQSDTTFSKQLVGQLKDSAVLDVQQDVASLDQARQKMSRSEID
                     AAIVLPPHFGAVAKTPNGTHPAGQLKVIYTQNNEQSGQALTSILRTQLSSINSKYVST
                     EVPFSVQGEQLNENSLSAFDYAFAGLLGFAIIGMGIFGPINVFPELKKMGILRRLSTT
                     PLRVWQYFLSIMLGQATTGLLSIAIMFVVAITVFNLQVVGNYFELAAFTIFGIFMILG
                     IGLALGGWAKNERQAAPLSNIIVFPMMFLSGTFFPRFLMPEWLQHVSSFIPLTPVIDG
                     IRMIATEGKHLVDLGPQLGLMAIWVVIIYVVAFKVFRWE"
     gene            complement(3023..3787)
                     /locus_tag="VFG56_01845"
     CDS             complement(3023..3787)
                     /locus_tag="VFG56_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6622655.1"
                     /translation="MKNTKNMVEVKDLVKVYGKKRAVDSISFNVKKGEIFGILGPNGA
                     GKTTTLEMLETLRHIDGGSAMIDGVDVKKNPYDVKRLIGVQPQTPAFQDKTKLIEVVQ
                     MFAAAYGEQVDAMKFLEDVDLSKKAGAYVEQLSGGQKQRLSITTALVHGPKVFFLDEP
                     TTGLDPQARRNLWSLIEKVRDRGISVIMTTHYMDEAEILCDRIAVMDEGKIIALDTPK
                     KLIKDLLGRGFKKKQHVEQANLEDVFIDLTGKALRD"
     gene            complement(3942..4148)
                     /locus_tag="VFG56_01850"
     CDS             complement(3942..4148)
                     /locus_tag="VFG56_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622656.1"
                     /translation="MAAYQHTNSKGVTYFLHKKDVTLRGGKNQTIYFFAKQEGGKGEP
                     TDLPAGYEVTENPRNGFLTIRKIR"
     gene            complement(4227..4874)
                     /locus_tag="VFG56_01855"
     CDS             complement(4227..4874)
                     /locus_tag="VFG56_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622657.1"
                     /translation="MVGKTHDKRHWAIELAKLRKIKTWQLLVILLLVSLLAATCLRLN
                     NLGMVTRRDAVMAADKAGDQQAIKSALVDLQSYVSHHMNTDLGQGVYLTESYERDRAA
                     AIDKAQSAKDAAGQAYRQADQECKQRFTGGVGSFRNDYVQCVINKTAGLEAANDKLKL
                     PQSELYRYDFASPRWSLDLAGLLVAIVALIILIIIVRTISVLILNLLLRRHFTAV"
     gene            complement(4896..6101)
                     /locus_tag="VFG56_01860"
     CDS             complement(4896..6101)
                     /locus_tag="VFG56_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6622658.1"
                     /translation="MIRRFIYNILQRRHFWRRVGFDELSELYVSTMLRSMSLSLIGIF
                     VPIYLYKLGYDLQLVFLFMICMFISRMFSDVLSGFLVARFGPKHVMLLSYIMQILSLA
                     LLLNLGHFKDLLWPSAIVYGLANSLFFIAYHVDFSKIIHADHGGKELGYMTILERIGA
                     AVGPLAGGLVAALFGAEYTIEAAIVLLAIAVIPLFMSAEPVRLRQKLRFRGLPIRALW
                     RDFVSFAAFGNSNTISVAIWPLYIAVAIFTVNTYAVVGLVTSISIVTAIVSARLIGQL
                     IDKRRGRQLLSASVFGGSILHLIRLMTSGFGGVTLVGMADQALTSGYKMPYSKGMYDR
                     ADSLPGYRIAYLVILETIGDCGKVAIWLVAWLASLWLTGTMAMQLCFIVAAFTILLIR
                     LERFKALKP"
     gene            6157..>6833
                     /locus_tag="VFG56_01865"
     CDS             6157..>6833
                     /locus_tag="VFG56_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET6622659.1"
                     /translation="MHIYFSGLGGVAIGPLAEIADGAGFTVSGSDLADNLMTHQLRQR
                     GVDVTIGQDGSQIAAVHAKSPIDWFVYTAALNEDHPELKFARDNDIRTSKRDEFLAEL
                     IKQKDLKLVAVSGTHGKTTTTGLLIWVFKQLGIPVSYSIGTTLSFGPSGQFDPDSQYF
                     VYECDEFDRNMLKFQPYLTLITAIDYDHPDTYPTEAEYRQAFVNFLEQSDYSLLWEKD
                     LRYLKANP"
BASE COUNT         1844 a         1732 c         1567 g         1690 t
ORIGIN      
        1 ggttatgttc gggggaatta actaaataaa tcgcagcgtc attgccgcca cttaaggctt
       61 cgatatgttc gggtcttaac tcatcaatcg acttcacccc aggcatcacg cgactggcaa
      121 taatcgattc tagatatttc ggggcaaacc ggcgaatttt tcgactcgac ggcggaatat
      181 ccggtttaat cggcagacga tctatctctt tggctttcat cggtgacctc ccggtttaaa
      241 aaccttcagc taccatttta ctattgttgg tcgtaaacat ctttacaagc gtcgcccact
      301 gtctttagat agtaggtgta atgccggata aaaatcctat aagaaaaaca tttaaccagc
      361 tgaaagccac ggtcaaaatc acacagttga ccgtccagcc tgtttccaag tcaccggata
      421 gagtattggg tgaactgttt caggccgttc agctaagtcg cctacaccct gacggcaaaa
      481 cttttgtcga tcaagtaccg agtgctcgta tgcgccaggt cgtaaggcgc tatcgtaaag
      541 aagccgacag gccagatttt gatctgtatg agtttgtcgc caaaaacttc gctgatttta
      601 cgcctagtag tcaatcagtt aaagctgagc ctagcactac ggccgaagat catatcatca
      661 gactgtggcc acacctgacc aagacggcct acaaatcgat cggctcactt atcgccctac
      721 ccaaccccta cgtagtaccg ggtggccgtt tccaggaaca attctactgg gatgcttatt
      781 tcatcatgct aggcttagag gtcagcggcc gtagcgacct aaccgactgc atgatgagca
      841 attatagcca tatgtttaga aaacttggtt tcatcccgac gggtaaccga acttacttca
      901 catccaggtc tcaaccgccg ttttatagtt tgatgatcga cctgatgatc cggcgcaaag
      961 gtaaaaagta tctaaccacc aggctgccct accttttaca ggaataccac ttctggaaca
     1021 agcattctcg cctgcccctg aagcctaagc gtcgcgtcaa actggacaag aacacttact
     1081 taaaccgtta ctacgacaat tacccctcgc cacgaccgga atcctactcg gaggacgtcg
     1141 agacagccaa actagccaaa gaccgtaccg ataagcaggt ctacttggac cttaaggcag
     1201 gggccgaatc cggctgggat ttttctagcc gctggttcga tgatcctatg aaaatcgaaa
     1261 ccatcagaac gaccaatctg attcccgttg atcttaattg cctactctat aaattggagc
     1321 tgaccatcgc tgattgttat cgcacattgc tccagttccc gctagccaag tactaccgtg
     1381 accgagcaac taagcgcaag gccgctataa atcactactt atgggatgaa tctaaaggct
     1441 ttttctttga ttatgatttc gtcaatcaca agaagtccga agattacacc ttggcgggtg
     1501 tctttgcact atacgtcggc ttggccgacg ataatcaagc cgccaaagtg gctggtgtta
     1561 tagaagataa gtttttaaaa gatggcggtg tcgtcacgac ccttcaagat accggacagc
     1621 aatgggattg gccgaatggt tgggctccct tgcagtggac gacaatcgtt ggactaaggc
     1681 gctatggtta tcacgaactg gccgacgcaa tcaagttccg ctggctagct accaacgagg
     1741 cgctctacct aaacgaaggt aagttcgtag agaagtacaa tgtcgtcgac cccaccgagc
     1801 gaggtggtgg tggcgagtat caactccaag acggtttcgg ttggaccaac ggcgttttct
     1861 tagcgcttaa aaaagacttc gacgtagagt tgagcgcctg attattccca gcgaaagacc
     1921 ttaaaggcga caacgtagat aatcactacc caaatcgcca tcaatccaag ttgcggacca
     1981 aggtcgacca agtgcttacc ttcggtagca atcattcgga taccatcaat aacgggggtt
     2041 agcgggataa agctcgatac atgttgtaac cattccggca tcaaaaaacg tgggaagaaa
     2101 gtgccggaca agaacatcat ggggaaaacg atgatgttag acaatggtgc agcctggcgt
     2161 tcattcttgg cccagccacc cagggctaag ccgataccga gaatcatgaa gattccaaag
     2221 atcgtaaaag cggccagttc gaaataatta ccgacaacct gcaggttaaa cacggtaatc
     2281 gccacgacaa acatgatggc gatagacaat agtcccgtcg tagcctgacc cagcatgatc
     2341 gacaagaagt actgccaaac acgcagtggt gtggtcgaca aacggcgtaa gatacccatc
     2401 ttcttaagtt cagggaagac gttgatcggg ccaaatatac ccataccgat gatggcaaag
     2461 cccagcagtc cggcaaaggc atagtcgaaa gctgacaggc tattttcgtt aagttgctcg
     2521 ccctgcacgc tgaacggcac ctccgtcgat acatacttgc tgtttatact actgagttgc
     2581 gtccttaaga ttgaggtcag cgcttgaccg gattgttcgt tgttttgggt gtaaataacc
     2641 ttaagctggc cagccgggtg agtcccatta ggcgttttgg ctacggcacc gaaatgaggc
     2701 ggcaaaacga tggcagcgtc aatttcagag cggctcatct tttgcctggc ttgatccagg
     2761 ctagcgacat cttgctggac atcaagcaca gcactgtcct tgagctggcc gacaagttgc
     2821 ttggaaaacg ttgtgtcgga ttggtttatc aatgctacgt tgaacgaaat atcaccgccg
     2881 ccgctgttta ggccaccaaa gacaaataag aatatgagcg gaaaaccgat tccgaaaaag
     2941 atcgacagct tatcgcggaa gaatcgtaat gtgttgattt tgtagaaaac taaaactgta
     3001 tagagcgatt ttggtaattt cattagtccc tcaaagcctt tcctgtcaaa tcaatgaaga
     3061 cgtcttcaag gttggcttgc tcgacgtgtt gcttcttctt aaaaccacgc cccagcaagt
     3121 ctttgatcaa ctttttcggc gtatcaagag cgatgatctt accttcatcc ataactgcga
     3181 tccgatcaca gaggatctcg gcttcatcca tatagtgtgt cgtcatgatg acggatattc
     3241 cacgatcgcg gactttttca atcaagctcc acaagttacg gcgggcctgt ggatccaagc
     3301 cggtagtagg ctcatcgaga aagaaaacct tcggaccgtg aaccagtgct gtcgtgatgc
     3361 tgagacgctg cttctgccca ccagacagtt gctctacgta tgcaccggct tttttactca
     3421 gatcaacgtc ttcgaggaat ttcatggcat cgacctgttc gccgtaagcg gcggcgaaca
     3481 tttgaacgac ttctataagt ttagtcttat cttgaaaagc tggggtctgc ggctgaacac
     3541 cgatcaagcg tttgacgtca taggggtttt tcttgacatc aacgccatca atcatggcgc
     3601 taccaccatc gatatggcga agtgtctcaa gcatctcaag tgtcgtcgtc ttaccagcac
     3661 cattcggccc taagattccg aaaatttcac cttttttgac attaaagctg atgctatcga
     3721 ctgctctctt tttgccatag actttgacca agtctttgac ttcaaccatg tttttagtgt
     3781 ttttcatctc gaatcattgt aacagaaaaa gtgtctctta ccagcggtgg ctataaggtg
     3841 atccaaaaag cccgcttgag attttcaagc gggcgatttt tggcaccttt ttgtttgtac
     3901 ataatagtac tccaaaaggc ttatgctttg caagtaccta tttagcgaat cttgcgaatg
     3961 gtcaagaaac cgttgcgtgg gttctcggta acttcgtagc cagctggcag gtcggttggc
     4021 tcacctttgc cgccttcttg cttagcgaag aaatagatag tttggttctt accaccgcgt
     4081 agggtaacgt cttttttgtg taagaagtaa gttacaccct ttgagttagt atgttgataa
     4141 gctgccataa agacactcct ccttttagtt gcttacgaga ttgatagtac gcttatctgt
     4201 taccccttgt caacagttta gcgcccctag acggccgtaa aatgccgcct cagtaacaag
     4261 ttaaggatta acacggatat ggttcgaacg atgataatca agatgatgag cgctactatg
     4321 gcgacaagca ggccagctaa atcaagtgac caccgcggtg aggcaaaatc ataacggtaa
     4381 agttcacttt ggggcaactt caacttgtcg ttggctgctt ctaggccagc tgttttgttg
     4441 attacacatt ggacatagtc attgcgaaaa gagccaactc caccagtaaa acgttgttta
     4501 cactcttggt ctgcctgacg ataagcctgt ccggccgcgt ccttagccga ctgtgcctta
     4561 tcgatagccg cggctcggtc tcgctcatat gactccgtca ggtatactcc ctggcctagg
     4621 tcagtgttca tgtgatgaga aacgtagctt tgtaaatcga caagtgcgga ctttatcgcc
     4681 tgctgatcgc ccgccttatc ggcggccatg acagcgtcgc ggcgcgtcac cataccgaga
     4741 ttgttaagcc ttaaacatgt tgccgccagc aaactaacta acaacaagat aaccagaagt
     4801 tgccaagtct taatctttct tagcttagct agctcaatcg cccagtggcg tttgtcgtgt
     4861 gtcttcccca ccatttgaat ttaagtataa caactttacg gtttaagagc cttaaaacgc
     4921 tccaaccgaa tcaacaggat agtaaacgca gctactatga agcatagttg catagccatg
     4981 gtacctgtta gccacaggct agcaagccaa gccaccaacc agattgcaac cttgccgcaa
     5041 tcacctatgg tttctagtat gaccaggtaa gcgatacgat agcctggcaa gctgtcggcc
     5101 cgatcgtaca tgcctttgga gtatggcatc ttatagccgg aagttagcgc ctggtcagcc
     5161 ataccaacta aggtgacgcc accaaagcca gacgtcatca atcgaatcag gtgcaagatc
     5221 gagccgccaa ataccgaagc actcaagagc tgtcgaccac ggcgcttatc gatcagctgg
     5281 ccgatcaacc tagcggacac gatagcggtc acgatactga ttgacgtgac tagtccgacc
     5341 acggcataag tattgaccgt aaagatagct acggcgatat atagcggcca aatcgccacc
     5401 gatatggtat tgctgttacc aaaagccgca aacgacacaa agtctcgcca caaggcccgt
     5461 ataggcaaac cgcgaaagcg tagtttttgc ctcaagcgaa ccggctcggc actcataaat
     5521 agagggatca ccgcaatcgc cagcaatacg atagccgctt cgatcgtata ctccgcgccg
     5581 aacaaggctg ccactaaacc gccggctagc ggtccgactg cggcgccgat acgctccagg
     5641 atagtcatgt agccaagctc cttaccgccg tgatcagcgt ggatgatctt agaaaaatca
     5701 acgtgatagg ctatgaagaa taagctgttg gccaaaccgt aaactatagc agacggccac
     5761 agtaaatctt taaaatgccc cagattgagt agtagtgcca gtgaaaggat ttgcatgatg
     5821 taactaagta gcatgacgtg cttagggccg aacctggcta ccaaaaaacc cgacagtacg
     5881 tcgctaaaca tccgagatat gaacatgcag atcataaaca aaaataccag ctgcaaatca
     5941 tagccaagct tatataggta gatcggcacg aatataccga taagactcag gctcatactc
     6001 ctgagcatag tactgacgta cagctcactc agctcgtcaa agccaacacg gcgccaaaaa
     6061 tgtcggcgct gcaatatgtt atagataaac cgtctgatca tgtttgtttt aacctgaatt
     6121 tcacccctat ttaaacactt tggttataat taggccatgc atatttactt ttccggacta
     6181 ggtggtgtag ccatcggacc actcgccgaa atcgctgacg gcgccggctt cactgtcagt
     6241 ggctccgact tagccgataa cttgatgacg catcaacttc gccaacgagg cgtcgatgtg
     6301 acgatcggcc aagatggtag ccagatcgct gccgttcacg ccaagtcacc aatcgactgg
     6361 tttgtctaca cggcggcttt gaacgaagat catccggaat taaagtttgc ccgagataac
     6421 gatatccgca cgtcgaagcg tgatgagttc ctggccgaac tgatcaagca gaaggatctg
     6481 aagctagttg ccgtttctgg aacccatggc aagactacta ccaccggttt attgatctgg
     6541 gtatttaagc aactcggtat tcccgtcagc tactctatag gcaccaccct ctctttcgga
     6601 ccaagtggtc agtttgatcc tgacagccag tacttcgtct atgagtgcga cgagttcgac
     6661 cgcaatatgc tgaagtttca gccctaccta acactcataa ccgcaatcga ttacgaccat
     6721 cccgacacct acccgaccga agccgaatac cgccaggctt tcgtcaactt tctagaacaa
     6781 tcggactatt ccctgctgtg ggagaaagat ctgcgctacc ttaaagccaa tcc
//
LOCUS       DASSBW010000064         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1763200, whole genome shotgun sequence.
ACCESSION   DASSBW010000064 DASSBW010000000
VERSION     DASSBW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763200"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFG56_01870"
     CDS             <1..439
                     /locus_tag="VFG56_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HET6622660.1"
                     /translation="SRGGALQLHINPAGDWSVGGFEADAGLTGRKLVVDNYGPRIPIG
                     GGCFSGKDASKVDRSAAYMARRIAVDYLKRFAAREVYCYLAYAIGVAKPVEATVVIDG
                     RSKSVMDYDLTPAGIRHRLELDRPQFEKTARYGHFGNGFAWDC"
     gene            451..888
                     /locus_tag="VFG56_01875"
     CDS             451..888
                     /locus_tag="VFG56_01875"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET6622661.1"
                     /translation="MNIQADNREDYFTQTGEREKALRRVDTVIQTAAPALTPVLFAGM
                     GGGAVLGYGLQPYQTKSMKQPTEWPLIVLANQKNYMALYICAVDTDGRYFAEKYADQL
                     GNVDCGRSCIRFKRLEDLNLPVVREVIGELARRVADGEKLFGV"
     gene            969..2237
                     /locus_tag="VFG56_01880"
     CDS             969..2237
                     /locus_tag="VFG56_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET6622662.1"
                     /translation="MANDKLKFPKHFFWGAATSAHQVEGGNHNQWSVWELENARALAS
                     AAPHKIGYLDGWDDIKAQATAPSNYVSGRATDHFNRYEEDFDLLEEMSLNAFRFSIEW
                     SRIEPKEGQFDSSAIQHYRDYLKSLKQRGIEPFVTLSHWTVPVWFDQKGGFERSGNIK
                     YFVRFAEKIVDELGPDVSYITTINEPDTVFVHGYITGQHPPQKNSWIVGLWVYINLLR
                     AHKRIYKLIKGRSRRFKVGFTKSYANVQAADASRLSRLSVSVDNFLRDNAVLSFVGKK
                     IDFLGVNYYFSDLHKGVGLTDHSPDTPRNDLGWEMLPDDLEIVLRRLGAKRPKTPIII
                     TESGVADASDKYRKWWLAHSLKAIDNAIRAGVRVDGYLHWSLLDNFEWAYGFWPHFGL
                     VEVDYQTMKRKLRPSAKWYGQVVKQMRGTK"
     gene            2234..3232
                     /locus_tag="VFG56_01885"
     CDS             2234..3232
                     /locus_tag="VFG56_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET6622663.1"
                     /translation="MIEEKLGSKIVVIGGGTGSFTLLNGLKHYARNITALVNMADDGG
                     STGHLRDELGVLPPGDIRQCLVALSDNPRVRELFNYRFDEGSLKGHAFGNLFLTALEK
                     MTGNFGHAVELAGQVLNITGQVEPITLADVTLCAEGSDGDELKGEFKISQASLKPRPR
                     VWLEPSAQANPAALQAIEEADLVIIAPGSLYGSLAPALVVDGIGQALQKTKAKRIYVS
                     NLVTKPGQTEGFDVTDFAAEIERLAGGPFLDYVIYNTLKPPKDLMERYAHEGEYLVEL
                     DQPKAENLNYQLSGRPILAKGGWGEAQAADEISATRSFIRHDSDAVARAIMRLYFD"
     gene            3225..>3560
                     /locus_tag="VFG56_01890"
     CDS             3225..>3560
                     /locus_tag="VFG56_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622664.1"
                     /translation="MTEELLLVDFDRTLFDTSKFSETWWRWAAEHYDIDIASELGRME
                     EYFNYVGQWRSYDFYRHIDELKLDVPTDQLLKQARDDLAGHDFTYADAKTVFKLKDQR
                     AMELVTFGNQ"
BASE COUNT          878 a          820 c         1000 g          862 t
ORIGIN      
        1 atcgcgtggc ggagcgttgc agcttcatat aaacccggcc ggcgattggt cggtaggcgg
       61 cttcgaggct gatgctggct taaccgggcg caaattggtg gtggacaact atggtccacg
      121 tataccgatt ggcggcggct gcttttccgg caaggacgcc agtaaagtcg accgctcggc
      181 tgcctacatg gctcgtcgaa ttgccgttga ctatctaaag cgttttgctg cccgagaagt
      241 ctattgctat ttggcttatg ccatcggcgt ggcgaagccg gttgaggcga cggtcgtgat
      301 tgacggtagg tcgaagtcgg tcatggatta tgacttgact ccggccggca ttcggcaccg
      361 gcttgaacta gaccggcctc agtttgaaaa aaccgctcgt tacggacatt ttggcaatgg
      421 ttttgcttgg gactgttaat ataaaagagc atgaacatac aagccgacaa cagggaagat
      481 tacttcactc agacaggtga gcgcgaaaag gcgctacgtc gggtcgacac tgtcatacag
      541 acggcggcac ccgccctgac gccggtctta tttgccggca tgggcggcgg cgcggtgctc
      601 ggttacggtc tgcagccgta tcaaactaaa agcatgaagc agccgaccga gtggccacta
      661 atcgtgttag ccaaccaaaa gaactacatg gcgctttaca tatgtgcggt tgacaccgat
      721 ggtcgatatt ttgccgaaaa gtacgccgat cagctcggca atgtagactg cggtcgtagt
      781 tgtatccgct ttaagcggct agaagactta aacttgccgg ttgtacggga agtcatcggt
      841 gagctggctc gtcgtgtagc cgatggcgag aagttatttg gtgtctagtt ctagtgggta
      901 tagccgcttt gactgatggt atggaatcgg atcaattcac tagaggcttg ctataatagt
      961 ggcagcttat ggccaatgac aaactaaaat ttcccaaaca cttcttctgg ggtgcggcca
     1021 ccagcgccca ccaagtcgag ggcggtaatc acaaccagtg gtcggtatgg gagctggaaa
     1081 atgcccgtgc cttggccagc gccgctccgc acaagatcgg ttatttggat ggctgggatg
     1141 acattaaggc tcaggcgacc gcaccaagca attatgtctc cgggagggca acggatcact
     1201 ttaatcgcta cgaagaggac tttgatttac ttgaggaaat gagtctaaac gcttttcgtt
     1261 tttcgatcga atggtcgcgg atcgagccga aagaaggtca gtttgattcc agtgcaattc
     1321 aacactaccg tgattatcta aagtcgctca agcaacgcgg tatcgaaccc tttgtgacac
     1381 tgtctcactg gacagtgccg gtttggttcg accagaaggg cggttttgag cggagcggca
     1441 atatcaaata ctttgtccgt tttgctgaaa agatcgttga cgaattaggg ccggatgtaa
     1501 gttacattac taccattaat gagccggata cggttttcgt gcatggctac atcaccggtc
     1561 agcacccacc gcagaaaaac tcatggatcg tagggctatg ggtttacatc aacttactgc
     1621 gagcccataa gcgtatctat aagctgatca aaggtcggtc aagacgtttt aaggtcggct
     1681 tcaccaagag ctacgccaac gtacaggctg ccgacgcctc tcggctgagc cggctatcgg
     1741 taagtgtcga taattttttg cgagacaatg ctgtactgag ctttgtcggt aaaaagatag
     1801 actttctcgg ggttaattat tacttttcag acttgcataa gggcgtaggc ctaaccgatc
     1861 atagcccgga tacaccaagg aacgacctag gctgggagat gttgccggat gacctggaaa
     1921 tagtcttaag gcgtctaggc gccaaacggc caaagacacc tatcatcatc accgaatccg
     1981 gtgtagccga tgctagcgac aagtaccgta aatggtggct ggcgcatagc ctgaaggcta
     2041 tagataacgc aatcagagcc ggtgtgcgag tcgatggata tctacactgg agcctgctcg
     2101 ataatttcga gtgggcttat ggtttttggc cacatttcgg tttggttgaa gtagattatc
     2161 agactatgaa acgaaagctg cgtcctagcg ctaagtggta cggccaagtc gtcaaacaaa
     2221 tgagaggtac caaatgatag aagaaaaact agggtccaaa attgtggtta tcggcggagg
     2281 tactggtagc ttcaccttgc tgaacggttt aaaacactac gctcgcaata tcacggcatt
     2341 ggtcaatatg gccgatgacg gtggttcgac cggccactta agagatgagt tgggtgtctt
     2401 gccgccgggt gatattaggc agtgcctggt tgcattatcg gacaatccac gagtcaggga
     2461 gttatttaac tatcgttttg atgagggtag tctgaagggc catgcttttg ggaacctgtt
     2521 tttgacggca ctggaaaaga tgactggtaa ctttggtcat gcggttgagt tggctggaca
     2581 ggtgttaaat atcaccggcc aggtcgaacc aattactcta gctgacgtga ctctgtgtgc
     2641 agaggggagc gatggtgatg agctaaaggg tgaattcaaa atctcccagg cttcactaaa
     2701 accacgaccg agagtgtggc tagagccatc tgcccaagct aacccggccg cgctgcaagc
     2761 catcgaagag gctgatctgg ttatcattgc tccaggtagt ctgtatggta gcctagcgcc
     2821 ggccttagta gtggacggta tcggccaagc attgcagaag accaaggcta aacgaatcta
     2881 cgtcagtaac ttggtaacca agcccggtca gaccgaaggt tttgacgtga ctgattttgc
     2941 cgctgagatc gagcgtttag ccgggggccc gttcctggat tacgtcatct acaatacgtt
     3001 aaagccgcca aaggatttga tggagcgcta cgctcatgag ggagagtatc tggttgagct
     3061 cgatcagcct aaggccgaaa atctcaacta ccagttgtcc ggccgaccga tattagccaa
     3121 gggtggctgg ggcgaagcgc aagccgccga tgagataagt gccacccgta gctttatccg
     3181 tcatgattcc gatgcggtag cccgagcaat tatgaggtta tattttgact gaggagttgc
     3241 tcttagtcga ctttgatcgg acgctgttcg atacgtcgaa gttttctgag acctggtggc
     3301 gctgggcggc cgaacattat gacatcgata tcgctagtga actagggcga atggaagagt
     3361 acttcaacta cgtcggtcag tggcgcagtt atgattttta tcgccatatc gacgagttaa
     3421 aacttgacgt gccaaccgac caactactca agcaggctcg agatgatttg gccggccacg
     3481 attttactta cgccgacgcc aaaacggtct ttaaactcaa agaccagcgc gcgatggagt
     3541 tagtcacctt tggcaaccag
//
LOCUS       DASSBW010000065         4417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1037113, whole genome shotgun sequence.
ACCESSION   DASSBW010000065 DASSBW010000000
VERSION     DASSBW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037113"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1404)
                     /locus_tag="VFG56_01895"
     CDS             complement(97..1404)
                     /locus_tag="VFG56_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622665.1"
                     /translation="MDQIESQEAQRQADFEAFEAERRLTDELTEASEKLAMPMRNRFD
                     YDLRPDGELYFEGQSLDEVFKTGVEVAEHLTKEQPEFYVEMVRRHLEYDEYQWMKRLA
                     AGRDDEPDLLVVVSPIPDAVVSGEVDLNAYDRERKKTLVRVFERTEQGLSATSLSLDL
                     SDRDGLREIAETLGDTISDDDSSEDILAKRLLGERRSFGDRSVARTLRRIYDQVLEDK
                     YGRSFLGGRPDDKSPEALSFIKRQTDLIEQHMAELDRLREIGASDKAIRRARHNFAAA
                     LDRRLSGEADAASLADAGDVAEARGQSFEGDCPTGVTATSSANSLGFGNKKQSFMSKS
                     CPICGASNVLTTIEGETISGSCGCQKEICSGKVTRINRPSQAPSNTAGSWSPTVEKPR
                     LPGKNQLIKQQFGEYAVIKTQLTIGGANRLVVDKRTGQLIAKL"
     gene            complement(1690..3339)
                     /locus_tag="VFG56_01900"
     CDS             complement(1690..3339)
                     /locus_tag="VFG56_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET6622666.1"
                     /translation="MLAVMCGLFLVALDQTIVSTALSSIVEEFNSFSSLSWVVTAYLL
                     GNTVTVPIAGKLSDLFGRRIVLMVGVAIFTITSFFSGSAADINQLIIARAIQGIGGGI
                     IMANAFTIVGDLFSPRERGRWQGLIGAVFAVSSVIGPLLGGFLTDHHTLFGLVTSWRW
                     NFWLNVPIGILAFAFIAKYCPRIRHDHKPAIDYLGAGTLTVALSSLILAVDNTDKIFA
                     GLIADGVSLGLIKVILYAAAAIFGAAFVWLETKAEEPIIPLDFFKNRTFRTIMAVALF
                     FGAAFLGAIIYLTQFNMQVFGVSATDAGLMLLPMVGGLTLTSALTGLLVTKTGKYKRF
                     FIGGLSLATISVFALTTLTPSSPYWHEAIIMAFVGIGLGTGMPIMNLAVKNEFEQKHL
                     GAATASSQLFRGLGSTVGVAVLGAMLTSGVTSHLGNIGDDAYIQRINSQPEAAKVLGD
                     GTANTVLNLNTKDAKDKIMDGLNQKLDQSNVSSETKQQIKQQFRADQDAFSAKVSSAF
                     SKSLRPIFYVSATLMAVATVVAWGVKEKPLRGRPDDTPGAE"
     gene            complement(3400..3645)
                     /locus_tag="VFG56_01905"
     CDS             complement(3400..3645)
                     /locus_tag="VFG56_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET6622667.1"
                     /translation="MTELSDKHVVLAGTATVGPKGQVVIPADVREKMGIAPGDKLVAL
                     YIPDKKSVGFVPESRLQQIIDKMGSHLESLKSLINEK"
     gene            3714..>4417
                     /gene="cysQ"
                     /locus_tag="VFG56_01910"
     CDS             3714..>4417
                     /gene="cysQ"
                     /locus_tag="VFG56_01910"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET6622668.1"
                     /translation="MDYPAVEQIIAIAAAAGDTIMEVYDSEDIDLTIKDDEHNSPLTR
                     ADLAAHDIINTELTKLTPNIPVISEESSDHDQNRTRQRLIWLVDPLDGTKEFIKRNGQ
                     FTVNIALIDQGAPVLGVVYAPALGLLYWADQDGAHKQVQNQPAEEIMVNLPRPNIKAA
                     VSASHPSPELSKWLDERNIKQTTAAGSSLKLCFVADGQVDVYPRLGPTMEWDTAAGDA
                     IIIAAGGVTLSHPELQP"
BASE COUNT         1144 a         1191 c         1062 g         1020 t
ORIGIN      
        1 cagcggcttc atcaaaaaac acggctacag ccatagtgct tttgaaaaat acaagaaaga
       61 cggcgtctta aagctcggtc gccaagcgct taagtcctag agtttggcaa tcaactgacc
      121 ggttcgcttg tccaccacca aacggttggc ccctcctatg gtcagctgag ttttaatcac
      181 cgcatactca ccaaactgtt gtttgatcaa ctgattcttg cccggtagtc ttggcttctc
      241 aacggttggg ctccagctac cagctgtgtt gctcggtgct tggctgggtc ggttgatccg
      301 ggtgactttg ccggaacaga tctctttttg acaaccgcaa gaacctgaga tcgtttcacc
      361 ttctatagta gtaagtacat ttgatgcacc gcaaatcgga caggactttg acataaagga
      421 ttgctttttg ttgccaaaac caagcgaatt agcggagctg gtggcggtaa caccagtcgg
      481 gcaatcacct tcaaaacttt ggccccgagc ctcagccaca tctccggcgt cagccagact
      541 ggcagcatca gcttcaccac ttagtcgccg gtctagagct gctgcgaaat tatgccgagc
      601 tcgtcgaatc gccttatcac tagcgccaat ctcccttaat cgatccagtt cagccatatg
      661 ctgctcgatc agatcagtct gtcttttgat gaagctaagc gcttccggcg acttgtcatc
      721 cggccgtcca ccgagaaagg accgaccata cttatcctct aaaacctggt cgtagattcg
      781 gcggagtgtc cgggctaccg accgatcacc gaagctccgt cgctcaccga gcaatcgttt
      841 ggccaggata tcctcacttg aatcatcatc actgatagta tctccaagtg tctcggcaat
      901 ttctctcaga ccatcgcggt ctgaaaggtc aagtgacagt gacgtagccg acaagccctg
      961 ttctgtccgc tcaaacactc gaaccaatgt ttttttgcgc tcacggtcat aagcattcaa
     1021 atcaacttca ccgctgacca cggcatcggg aatcggtgag acgacaacca gtaggtcagg
     1081 ttcatcgtcc cgaccagccg ccaagcgctt catccattgg tattcatcgt attccaaatg
     1141 acgacgaacc atctcgacgt aaaactccgg ctgttctttt gttagatgtt cagctacttc
     1201 aacaccggtc ttaaaaacct catccaatga ttgaccttcg aagtatagtt cgccatctgg
     1261 tctaaggtca tagtcgaaac ggttacgcat gggcatagcc agtttttcac tagcttcggt
     1321 cagttcatcg gtcaggcgac gctcggcttc aaaagcctca aaatcagcct gacgctgagc
     1381 ctcctgtgac tcgatctgat ccattacact ccccatcaaa aaacctcttc ctcccgggac
     1441 gggtagaaag aggcttaaac cgctaaccac acttcctgct cgggaaagcc cttttaggtt
     1501 accgacgcgc tcggacttat ccagtgacaa ccctcattct cactcgattc accgttgcgg
     1561 cacagttcgg gacttacacc cgattcagcg ccggtaacat aggctctgtt tgttaatggt
     1621 tccaagtata gcacgctatg actagcgtca tctagcgtta aaaatacacc aggtttagat
     1681 gagaaccgtt tactctgcgc caggcgtatc gtcgggtcgt ccccgcagag gcttctcttt
     1741 gacgccccat gccaccactg tggcgaccgc cattagagta gctgagacat agaaaatcgg
     1801 acgcagggac ttagaaaagg cgcttgagac tttggcactg aaggcatctt gatcagctct
     1861 gaattgttgc ttgatctgtt gttttgtctc ggaagaaacg ttggactgat ccagcttctg
     1921 gttaagcccg tccatgatct tgtctttagc atccttggtg ttgaggttga gtaccgtatt
     1981 ggcggtacca tcgcccaaga ccttagcggc ttcaggttgg ctattgatcc gttgtatata
     2041 agcatcgtca ccgatattgc caaggtgaga agtcacgcct gaagtcagca tagcgccgag
     2101 tacagccaca ccgacagttg aaccaagtcc cctaaataac tgtgacgaag ccgtggcagc
     2161 gcctaaatgt ttttgctcga attcgttttt gacagccagg ttcatgatcg gcataccggt
     2221 acctaagcca ataccgacga acgccatgat gatagcttca tgccagtacg gtgatgacgg
     2281 cgtcagggtg gttagtgcga aaacactgat agtagctagg cttagaccac cgatgaaaaa
     2341 gcgtttgtat ttgccggtct tagtaaccag cagaccagtt agagccgacg tcagagtcaa
     2401 accgcccacc atcggcaata gcatcaggcc agcgtcggtg gcactgacac caaatacctg
     2461 catgttgaac tgggttaggt atatgatggc acccaagaaa gctgcaccaa agaacaaagc
     2521 gaccgccatg attgtcctaa acgtccggtt cttaaagaaa tccagcggga tgataggctc
     2581 ttcggctttg gtctccagcc aaacaaaggc ggcgccgaag atagctgcgg cagcgtagag
     2641 aatgaccttg atcaagccca ggctaacgcc gtcggctatc agaccggcga aaatcttatc
     2701 ggtgttatca acagccagga tcagactcga taaggccacg gtcagggtac cggcaccgag
     2761 gtaatcaatg gccggcttgt gatcatgacg gatgcgtgga cagtacttag cgatgaaagc
     2821 aaaggccaat ataccgatcg gcacatttag ccaaaaattc cagcgccatg acgtaaccaa
     2881 accgaacaat gtgtggtggt cggtcaggaa gccacccagt agcggaccta tgactgaaga
     2941 gactgcaaac acagcaccga tcaagccttg ccagcgacca cgttcacgcg gagaaaataa
     3001 gtcaccgacg atagtaaaag cgttagccat gatgataccg ccaccgatac cttggatggc
     3061 ccgggcaata atcagctggt tgatgtcagc agcgctgcca gagaaaaatg aggtgatagt
     3121 aaagatcgcc acgccgacca ttaggacgat acgacggcca aacaagtcag ataacttacc
     3181 ggcaatcggc acagtcacgg tattgccgag taagtaggcc gtgaccaccc aagacaggct
     3241 agagaagcta ttgaactctt cgacgatact cgatagcgcc gtggaaacga tagtctggtc
     3301 tagtgctacc aggaaaagac cgcacatgac ggccagcatt gtgattattt tttggcgcag
     3361 cggtatatga tgtagcacga tttctccctg ttttttctct tacttttcgt tgatcagcga
     3421 cttgagcgac tcgagatgcg agcccatctt gtcgattatc tgctgtaggc gggattcagg
     3481 cacgaagccg acggattttt tgtcagggat atataaagcc actagcttgt cgccgggtgc
     3541 aatacccatc ttttcgcgta catcagccgg aatcaccact tgacctttgg ggccaacagt
     3601 ggcggtacca gctagcacca catgtttatc tgataattca gtcacggtta tacattttat
     3661 acatgtataa cttggctgtc aaacaatcac gccaagcttg ttatactagc acaatggatt
     3721 acccagccgt agaacaaatc atcgccatcg ccgcagcagc cggtgatacc ataatggaag
     3781 tctatgacag cgaagacatc gacctgacca tcaaagacga tgagcacaat tcgccgctga
     3841 cgcgggctga tctagcagct catgacataa tcaacaccga actaaccaag ctaacaccta
     3901 acatcccggt gatctctgaa gaatcgtccg atcacgacca gaaccgaacc cgacaacgat
     3961 tgatctggtt ggtcgatccg ctagacggta ccaaggagtt tatcaagcgc aacggccagt
     4021 ttacggtcaa tatcgccttg attgatcagg gcgcaccggt gctgggagtg gtttatgctc
     4081 cggcactcgg cctgctttac tgggctgacc aagatggagc ccacaaacaa gtccagaacc
     4141 agccagctga agaaatcatg gttaacttac cgcgacctaa tatcaaagcg gcggtcagcg
     4201 cttcacaccc tagcccagaa ttatctaagt ggctagatga gcgcaacatc aagcagacca
     4261 cggcagccgg ctcatcactc aaactatgct tcgtcgccga cggccaggtt gatgtttacc
     4321 ctaggctggg cccgactatg gaatgggata cggctgcagg cgacgctata attatcgccg
     4381 caggcggtgt aacgctcagt catcccgaac tgcaacc
//
LOCUS       DASSBW010000066         8255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1249940, whole genome shotgun sequence.
ACCESSION   DASSBW010000066 DASSBW010000000
VERSION     DASSBW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249940"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VFG56_01915"
     CDS             complement(<1..460)
                     /locus_tag="VFG56_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622669.1"
                     /translation="MKNQLKKIKLPRRKAAEEPERPERITNDTVSKHRERILAGGRRF
                     KYPIQYAKHKLIINSIIITVVALIVMALLIWHQLYVAQNTSKFIYRVTQLIPLPVASV
                     DGQPVRYSDYLRDLRADIYSSKNLNQIDLSAGDQQINYLKRQELTKAERGA"
     gene            complement(444..953)
                     /locus_tag="VFG56_01920"
     CDS             complement(444..953)
                     /locus_tag="VFG56_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6622670.1"
                     /translation="MAEFDGQRDDETVEFVFRRHIIAMRKGFYGLLIPFVFGSLPFLI
                     FPDNLQLLWGALAGLGLGLLLFFYHWIGWYYSIFIVTNQRVQQTSQSGLFGKTVIDVS
                     LNKVQNISYNVPGMTGEILGFGTIVLQTYVGDMVIEKAQHPEKIYNKLQDLLHLQTNQ
                     SQGLDEEPA"
     gene            complement(999..1073)
                     /locus_tag="VFG56_01925"
     tRNA            complement(999..1073)
                     /locus_tag="VFG56_01925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1038..1040),aa:Arg,seq:cct)
     gene            complement(1194..1691)
                     /locus_tag="VFG56_01930"
     CDS             complement(1194..1691)
                     /locus_tag="VFG56_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET6622671.1"
                     /translation="MIERTLVVFKPDAVQRGVVGEILTRFERVGLKIVGTKMIAPDKD
                     HYFTHYEGIGKMISRRGQEAFDLTLEFMTQGPVIAFVLEGVEAVEEVRKMVGGTAPKD
                     ALPGTIRGDYAHISFGYADDTRKGIPNLLHASGDAQEAKAEIDHWFKSEELFDYQSMH
                     EKYTR"
     gene            1839..2732
                     /gene="xerA"
                     /locus_tag="VFG56_01935"
                     /pseudo
     CDS             1839..2732
                     /gene="xerA"
                     /locus_tag="VFG56_01935"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
     gene            complement(2729..3799)
                     /locus_tag="VFG56_01940"
     CDS             complement(2729..3799)
                     /locus_tag="VFG56_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622672.1"
                     /translation="MVNKNQQTGFVSIMVVIFFVLLASVLTIGFARLVQMGQQQALAD
                     QESKLAYQSAQAGIEDAKRAITYCANNNMADCNQLFDDKCPGFYANNRFSSLGLSADS
                     QDAIHVGDQRVNQRYTCVVINDLIDRYWGELDPSSSGHKSILLPLKSDKSFDRAVISW
                     GMKVAGGSYNFPSSGAASKNPPKSQWVSQNWPAMMRIMLIEVPSGSMYPSDIINQSSF
                     LAPSFGSGDTTVLAQDLPVREIVKCSNGGSTNYACNVTLDLSGKSMITNDYYLELTPL
                     YRKTDFEVKLYNGALNRQIKSSFYTIDAVGAVENTYRRVQVMIGPANVVSPSDALQTG
                     KTICKDFWVTGYYNSYQAGDSC"
     gene            complement(3810..4466)
                     /locus_tag="VFG56_01945"
     CDS             complement(3810..4466)
                     /locus_tag="VFG56_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6622673.1"
                     /translation="MIRSTRRRQSGFTLVELTLAMAFLSTIFLLIALVTMQLMAIYNR
                     GLAIKQINEVGSSIMSDINHVSNSGINTQFSAGPNFICIGDTGYYWNTMQTLNSDGPH
                     YYFTDDSQPIGVIKTNNTTDDAGLNCSGGAMGTNKPVDRNQVTSLVGDTVRVISLDTR
                     SDSDNNHVHQLSLTIGTYTEDGSNQPIFKDGRWQCPPSSIGQFCAVDTFKTVIYLPNS
                     "
     gene            complement(4450..4983)
                     /locus_tag="VFG56_01950"
     CDS             complement(4450..4983)
                     /locus_tag="VFG56_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6622674.1"
                     /translation="MLKQRGDTLIEVLLAMGVLSLAVVGCMSIMNFSFSSSLDTTMRT
                     KTLALISGQANLLKSARDNYVHSVNVDDWKNIRSHSADPGLAESEPCQVGGNGNSRFY
                     LNANAASHWAQLQTVSGGATGSPQPSLGNGIWVEAYRHNPLNGPDFYDFYIKACWQGN
                     RAQQQLVSVVRLYDPID"
     gene            complement(4977..5762)
                     /locus_tag="VFG56_01955"
     CDS             complement(4977..5762)
                     /locus_tag="VFG56_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6622675.1"
                     /translation="MNGQKGFTIIEVLLFVAVSALLLAGVMAGITTNLNSTRFLASTK
                     SLENYLQKQYDDVLAGVAERHTDASCPYSGGGGATQPPGASDGCVVIGKLVRFTDKQL
                     LVFPVVSQMEPTTLCHADGNGDALAIGRYCPITLSSNRGVDVAEYEYQWGVSVAEGNK
                     LQPNGNPADYNALAILRSPASGNMYTFSFYIPGNTLTQPTQTRSLYSGDLDKENLNRP
                     AALCLQSDTAVTRTSYIYLTGGLGADAVRSGDNPSGLPEGLTC"
     gene            complement(5810..6619)
                     /locus_tag="VFG56_01960"
     CDS             complement(5810..6619)
                     /locus_tag="VFG56_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET6622676.1"
                     /translation="MVIDVWLALALGYLGAVFGSFAGATTWRLKHRKDIVKDRSECEQ
                     CGHKLGALDLIPVLSWLVLRGKCRYCHKPIGWTALMFEVGLGLAFALTYVFWPGDLAT
                     GLDWTALGLWLISLVLMAILFAYDLRWYILPDVVMAPLVAAGLGIFLVRASQSGWSVE
                     QAALELVLSLLPVTGLYGVLFAVSRGRWVGDSDPILGIFIGLALGWQLALLSVVVANF
                     IGLLAVLPQLASSRLSAKDQVPFGPFLILATLFCLWFGQSVIDWYFGWLLV"
     gene            complement(6692..7162)
                     /locus_tag="VFG56_01965"
     CDS             complement(6692..7162)
                     /locus_tag="VFG56_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6622677.1"
                     /translation="MSNKTNQPKGFTIIEVVLVLAIAALIFLLVFLALPALQRSRRDT
                     QRRDDVSRLLGQVTTWQANNNGQVPATDDDLDSLSVDYLKSADGEFNDPQTGSAYTLT
                     LSSVGDGTPADLGIMNYFKGAKCVNASTTESGTGNQVAVIVKLEGSPSYCVDNS"
     gene            complement(7276..>8255)
                     /locus_tag="VFG56_01970"
     CDS             complement(7276..>8255)
                     /locus_tag="VFG56_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6622678.1"
                     /translation="LPLAQGLHTVQEQTTNKRLKVVVQDIITSVEGGSSLAKAFAKHP
                     ELFDEIYIALIAAGEVSGTLDKSLERIADQQEKDAAIASKIRGAMVYPMIVVFVMLGV
                     VIFMLTTVVPQIEILYDNLNKELPTLTAIMVGAADFLTRFWWLIIIIIGLGVFYLRRY
                     SKTPAGIRTFDTLKINMPLFGEMFRKLYMARFTRTGQTLLVSGVQMLEMLRISSQAVG
                     NVLVAESITRASEKVKSGKSLATSLKNEEYILPLVYQMISIGEQSGGVDAMMGKAAGF
                     YENELDDAIKAISTSIEPILMVLLAVVAGGMVGAILFPVYKLSSTTSLG"
BASE COUNT         2079 a         2140 c         1992 g         2044 t
ORIGIN      
        1 aagcgccgcg ctcggctttg gtcaactcct ggcgcttcag gtaattaatc tgttgatcac
       61 ctgcgctaag atcaatctga ttaaggttct tagacgaata gatatcagcc cttaagtccc
      121 gtaagtaatc actgtagcgt acgggttggc catcgacgct ggccaccggc agtggtatta
      181 actgtgtcac tcggtagata aacttagacg tgttctgcgc aacgtagagc tgatgccaga
      241 tgagaagcgc cataacgatc aaagccacca cggttatgat gatgctgttg atgatcagtt
      301 tgtgcttggc gtattgaatc gggtacttaa accgtcggcc accggccaaa atacgctccc
      361 ggtgcttaga taccgtatcg ttggttatcc gctccggtcg ttccggctcc tcggccgcct
      421 ttcgcctagg tagtttgatc tttttaagct ggttcttcat ctaatccttg tgattgattg
      481 gtttgcagat gcagcaggtc ctgcagcttg ttatatatct tttctggatg ctgggctttt
      541 tcgatcacca tgtctccgac gtaggtctgc aagacgatgg taccaaagcc caatatttcc
      601 cccgtcatac ctggtacatt atagcttata ttctgaactt tgtttaagga cacgtcgatg
      661 accgtcttgc cgaatagacc ggattgtgat gtctgttgca cgcgttggtt tgtcacaata
      721 aaaatcgaat aataccagcc gatccaatga tagaaaaata gcaatagacc aaggccgagg
      781 ccagctaagg caccccatag taattggaga ttgtccggga agatcagaaa tggcaaagat
      841 ccgaacacga acgggattag cagcccataa aaacctttgc gcatagcgat gatgtggcgg
      901 cgaaagacaa attctaccgt ttcatcgtcg cgctgaccgt caaattctgc cataagctta
      961 ttgtaatcta tttggtgtca actatttgac taatattgtg gtgtcccgac tacgaatcga
     1021 acgtagatct attccttagg agggaactat tctatccatt gaactaccgg gactcgtcca
     1081 agtaagctgt cggcttccgg gagcgaatga aatccgctcc cttcaagctt atcgctactt
     1141 ctcctctcgg tgcaacagac agctagcttg gtttgttgca ccggctagcc gcattagcga
     1201 gtgtattttt cgtgcatcga ctgatagtcg aacagttctt cggacttgaa ccagtggtcg
     1261 atctcggcct tggcctcttg tgcgtcgccc gaagcatgca acaaattcgg tatacctttg
     1321 cgggtatcat cggcgtagcc gaagctgatg tgggcatagt caccgcggat agtacccggc
     1381 agagcatctt ttggtgccgt gccaccaacc atcttgcgga cttcctctac cgcttcgacc
     1441 ccttccaaga caaaggcaat caccgggcct tgagtcatga actccagtgt caggtcaaag
     1501 gcctcttggc cgcgcctcga gatcatctta ccgatgcctt cgtagtgagt aaaataatga
     1561 tccttatcgg gagcgatcat cttggtgcca acgattttta aaccaacccg ctcaaatcga
     1621 gtcagtattt cgccgactac cccgcgctgc acggcgtcag gtttaaacac aactagagtt
     1681 ctttcaatca tggacaatag ttttccttgt tttacacgaa ttatttatca gccttaattg
     1741 taacagagat gggcgcctcg cccaagctgt cagtgtcgca aaatattagc acaagcgtta
     1801 aacttaaggg tatgtatttg tccgagctga tccaagactt tgttgaatac gtagaagtcg
     1861 aacgtggccg aagccaaaag acagccgaga actatcatct ctacctccag cggctagtcg
     1921 aattcgccga cgacatcact gtcgataaga taaatcctga gttagtccgt aaatggcgcc
     1981 tatggctcaa tcgctatcaa aacgagactg gtcaggagtt gagccggttg actcagagct
     2041 atcacttgat cgcccttagg agtttcttga cctacctatc gaaacgcgag atagctagcc
     2101 tgactcctga aaaagtcgag ttgcccaaga ccaaacgaaa acaggtcagt ttcctgacac
     2161 aagaagaaac tatccgcttg atcgaagtta ttgacactac ttcacctcag gggatgcgtg
     2221 atcgtgccat agttgaacta cttttttcgt caggtctgcg cgtctcagag ttagttggcc
     2281 tagatatcgg tcacctcaat ttgaagcgcg gcgaattcat ggttcgcggt aaaggagata
     2341 aggatcgacc ggttttcttg agccctgagg cgaccgagtg gctagcccac tacctggagt
     2401 cccgccacga tgatttcaag ccactattta taagatacgc cggcgccaac acctctgaga
     2461 cgggtgacga ggcggtcagg ctgaccgccc ggtctgttca gcgcatcgtc agccgttatg
     2521 ccaggatggc tggcataact aagcacgttt ctccccatac gctccggcac agtttcgcta
     2581 ccgatttact gatgaatggc gccgacctga gaagcgtcca gtcgatgctt ggtcactcca
     2641 atatcgcaac cactcaggtc tatactcatg tcaccgacca gcacctaaga gaggttcacg
     2701 ccaaatttca ccgcagccaa gctaacgatt agcaactatc gccggcttga tagctgttgt
     2761 aataaccggt aacccagaag tccttgcaga tggtcttgcc cgtctgcaag gcgtcactag
     2821 gcgatacaac attggctgga ccgatcatga cctgaacccg tcgatatgta ttttcgaccg
     2881 cccctaccgc gtcgatggtg tagaacgagc tctttatctg acgattcaag gcgccgttgt
     2941 agagcttgac ctcaaagtca gtctttcgat atagcggagt taactcaaga tagtaatcgt
     3001 tagtgatcat cgatttaccg cttaagtcca gagttacgtt acaagcgtaa ttagtcgaac
     3061 cgccattgct gcacttgact atctctcgaa ccggcaagtc ttgagcgagg acggtagtgt
     3121 cgccgctgcc gaaactaggt gctaaaaagc tggattgatt gatgatatcg gaaggataca
     3181 tcgagccgga tggtacttcg atcagcataa ttctcatcat ggccggccaa ttctgagaca
     3241 cccactggct cttcggtggg ttcttagaag ccgctccgct acttgggaag ttgtagctac
     3301 ctccggctac cttcataccc catgagatga ccgcccgatc gaacgacttg tcgctcttaa
     3361 gcggtagcag gatagactta tgtcctgatg agctaggatc aagctcaccc cagtagcgat
     3421 ctatcaagtc gttgataaca acgcaggtgt aacgctggtt taccctttga tcaccaacat
     3481 ggatcgcgtc ttggctatcc gcactcaagc caagcgaact aaacctatta ttggcgtaaa
     3541 agccaggaca cttgtcgtca aacaactgat tacaatcggc catattattg ttggcacagt
     3601 aagttatggc ccgcttagcg tcctctatgc cggcctgagc cgattgatag gctaacttgc
     3661 tctcttggtc ggccagggct tgttgttggc ccatctggac taacctggca aagccaatcg
     3721 tcagcacgct agctagtagc acgaagaaaa tcactaccat gatagagacg aaaccggtct
     3781 gctgattttt atttaccata agcatattat taactattcg gaagataaat tacagtctta
     3841 aaggtatcga cggcgcagaa ctggccaatc gaactgggcg ggcactgcca cctgccgtct
     3901 ttgaagattg gctggttgct accgtcttcg gtgtaggtgc ctatagtcag gcttagctga
     3961 tgaacgtggt tattgtcact atcacttctg gtatcgaggc tgattaccct gacggtatca
     4021 ccgactaggc tggttacttg gttccggtcg actggtttat tggtacccat cgcaccaccg
     4081 ctacagttta ggcctgcatc atcggtggtg ttattagtct tgatcacacc gattggctgg
     4141 ctgtcatcgg tgaaataata atgcggcccg tcgctgttaa gagtctgcat ggtattccag
     4201 tagtaaccgg tgtccccaat acagataaaa ttcggtccgg cgctaaattg agtattgata
     4261 cctgagttgg atacatggtt tatgtcagac atgatacttg atccgacttc gtttatttgc
     4321 ttgatggcta ggccgcggtt gtatatagcc atcaactgca tagtgaccaa agctatcagt
     4381 aagaagatag tcgacaaaaa cgccatagcg agggtcaact caaccaaagt aaaaccgctt
     4441 tgacggcgcc tagtcgatcg gatcatacaa cctcactacg ctgaccagct gttgctgggc
     4501 tcgattgccc tgccagcagg ccttaatata aaaatcataa aaatccggtc cgttgagcgg
     4561 attgtgacgg taggcctcta cccatatgcc gttgccgaga cttggttggg gcgaacccgt
     4621 ggctcctcca ctgacggtct gcagctgagc ccagtgactg gcggcattgg cattgagata
     4681 aaaccggcta ttaccattgc cgccgacttg gcacggctca gactcggcca gtccaggatc
     4741 ggcactatga ctgcgaatat ttttccaatc atcgacattg acactgtgaa cataattatc
     4801 tcgagccgat ttgagcaaat tagcctgacc gcttatcagg gctaaagtct tagttcgcat
     4861 agtcgtatcc agactgctac tgaagctgaa attcatgatc gacatacagc cgaccaccgc
     4921 tagagacagc actcccatag ctagtagtac ttcgatcagc gtatcaccgc gctgttttaa
     4981 catgtcagtc cctccggtag gccgcttggg ttatcgccgg accggacggc atcggcacct
     5041 agtccaccgg tcagatagat gtaactggtc ctggttactg ccgtatctga ttgcaggcac
     5101 agtgccgctg gtcgattgag gttctcctta tctagatcgc cggagtacag agatctagtt
     5161 tgggtaggtt gagtcaaggt gttgccaggt atatagaagc tgaaggtgta catattgccc
     5221 gaagccggtg agcgcaatat ggctagggcg ttatagtcgg ccggattgcc gttgggctgc
     5281 aatttgttac cctcggcaac tgacacgccc cactgatatt cgtattcggc cacgtcgaca
     5341 ccgcgattgg agctcagggt aatcggacag tacctgccta tagctagagc gtcgccattg
     5401 ccgtcggcat ggcacagtgt cgtcggttcc atctgagaaa ctaccggaaa gaccaatagc
     5461 tgcttgtcgg tgaacctaac cagcttaccg atgactacac agccgtcgga ggcacccggt
     5521 ggctgtgtcg ctccgccacc accagagtaa ggacagctag catcggtatg ccgttcggct
     5581 acaccggcca gtacatcgtc gtattgcttc tgtaggtagt tctctagact cttagtcgat
     5641 gccaaaaaac gcgtactgtt gaggttggta gtgataccgg ccattacgcc ggctagtagc
     5701 aaagcgctca cagctacgaa cagtagaacc tcgataattg tgaagccttt ttgcccattc
     5761 atcttaaagc caattatagc ataagcttta aaatgtctta tctgttagat cagaccagta
     5821 gccagccgaa ataccaatcg ataaccgact gaccgaacca taggcaaaac aatgtcgcta
     5881 agatcaaaaa cggcccgaac ggcacctggt ctttagcact cagacgcgag ctagctaact
     5941 gaggtagcac agccaatagg ccaatgaaat tagccaccac cacactaagc aaggccagtt
     6001 gccagcccaa ggccagtcct atgaatatac ccagtatcgg gtcgctgtca ccgacccagc
     6061 gaccacgact gacggcaaac aggacaccgt agaggccggt caccggcagt aggctgagca
     6121 ccagctctag cgccgcttgt tctactgacc agccgctttg gctagccctt actagaaaaa
     6181 tacccaaccc ggctgccacc agtggcgcca taaccacatc cggcaagata taccacctga
     6241 ggtcataagc aaacaaaata gccataagaa ccaggctgat cagccataac ccaagcgctg
     6301 tccagtccaa accggtagcc agatcacccg gccagaagac ataagtaagg gcgaaagcca
     6361 gacctaaccc tacttcaaac ataagagccg tccaaccgat aggtttgtgg cagtaacgac
     6421 acttccccct caagaccaac cagctaagta ccggtatcaa gtctagcgca cctagtttat
     6481 gcccgcattg ttcgcactca gaccggtcct taacgatatc tttgcggtgt tttaaccgcc
     6541 aagtcgtcgc accggcgaag ctaccgaaga cagctcctag gtaaccaaga gctagcgcta
     6601 accacacatc tataaccata actagtattt taccctaaac tacttaaaaa cgctccagcg
     6661 gagcgttttt aagtctatgt tctatagaga actagctgtt gtcgacgcag taagaaggtg
     6721 aaccttccag tttgactata acagcaactt ggttaccggt acccgactcg gttgtactag
     6781 cgttaacaca cttagcaccc ttgaagtagt tcataatacc taggtcggct ggcgttccat
     6841 caccgacgct cgataaggta agtgtatagg cactaccggt ctgcgggtcg ttaaactcac
     6901 cgtcagctga ttttaggtag tccacactaa gtgagtccaa atcatcatcg gtagctggca
     6961 cttggccgtt gttgttggcc tgccaagtcg taacttggcc caggagacga ctgacgtcat
     7021 cgcgacgctg tgtgtcacgg cgtgagcgct gcaaggccgg caaagccaag aataccaaca
     7081 ggaagatcaa ggccgcgatc gctaagacca agaccacctc gatgatggta aaacctttag
     7141 gttgatttgt tttgttagac ataggttagt ccaccctttc tatgcttgtt attaaataga
     7201 cttgtgtgcc tatcataatg cttatgatta tagccctgtt gttttataat gcaaggcttt
     7261 ttccaccgct gtgatctagc cgagtgacgt agtactggac agcttgtaaa ccgggaacaa
     7321 gatagcgccg accataccac cggctaccac ggctaacagc accatcagga taggttcgat
     7381 cgaggtcgag atagccttga tggcatcatc cagctcattt tcatagaaac cagccgcctt
     7441 acccatcatg gcgtcgacac caccggactg ctcaccgata cttatcatct ggtagaccag
     7501 tggtaggatg tattcttcgt tcttgagcga ggtggccagt gatttaccgc ttttaacctt
     7561 ttcgctagct cgggtgatcg actcggccac taagacgttg ccaaccgcct gagaggagat
     7621 ccgcagcatc tctagcatct gcacaccact gaccagtagg gtctggccgg ttctggtaaa
     7681 cctggccata tacagcttcc tgaacatttc gccaaatagc ggcatgttga tcttcagggt
     7741 atcgaaggtc ctgatgccgg ccggggtctt gctataacga cgaaggtaga aaacaccaag
     7801 accgatgatg atgatgatca gccaccaaaa acgggttaag aagtcagccg ctcccaccat
     7861 gatggcggtc aatgtcggca gttccttgtt taagttatcg tagagaatct caatctgtgg
     7921 caccacagtg gttagcataa atataaccac gcccagcata acgaagacta cgatcatcgg
     7981 ataaaccatg gcgccgcgga ttttggaggc gatggcggcg tctttttcct gttggtcggc
     8041 gatccgctcc agtgacttat cgagcgtacc agagacttca ccggcggcga ttagggcgat
     8101 atatatctca tcgaagagtt ctgggtgctt agcaaaagct ttggccagag atgaaccacc
     8161 ctccaccgaa gtgatgatat cttgtacgac aactttgagc cgcttattgg tagtctgctc
     8221 ctggacggtg tgcaggcctt gagcaagagg caggc
//
LOCUS       DASSBW010000067         7293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_719141, whole genome shotgun sequence.
ACCESSION   DASSBW010000067 DASSBW010000000
VERSION     DASSBW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719141"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1992
                     /gene="pnp"
                     /locus_tag="VFG56_01975"
     CDS             <1..1992
                     /gene="pnp"
                     /locus_tag="VFG56_01975"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET6622679.1"
                     /translation="GDTVVLGTAMVGSKPISGMDYFPLSIDYEEKMYAAGKISGSRFI
                     KREGRPSEEAVLIGRIIDRPIRPLWPKGYRQEVQGVASVLSMDPNFRPDMVAMVAVSA
                     ALSLTGAPFDGPVAGLRVGLDEKGDFKAFMSREDMEDNQLDLVVAGTKAGVMMVEAGA
                     REVSEEKLVEAMDWAQHNMQPAIKLQQELVKKLAPKSLEYELVLPDENIQKEADKWVE
                     GKLGEDLRKPYPERNEVIGIMRGEFHKAFEEKLGEDYPELKAEYDEAFTKALHNDVRD
                     GIVRDGIRPDGRELTEIRPLSSEISILPRAHGSSIFTRGVTQGMNIVTLAPLSFAQMV
                     DTMEVTDGERRYMHHYNAPGYTVGEVRRLGSPGRREIGHGYLAERALIPVLPTAEEFP
                     YAIRSVTEIMSQNGSTSMAATCSSCLALMDAGVPISKPVSGIAMGLMMDGDKPYVMSD
                     IADAEDFAGDMDFKVTGTKDGVTALQMDMKVHGLPVNILKIALEQGKQGRAHILEHML
                     TTLAEPRNTLSPYAPQIEKIKINPEKIGSIIGKGGETINKITSETGAQVDIKDDGLVT
                     VASSDLESIKKAVDWIKSLVEEPEVGKVYEGPVVKVMEFGAFVNIMPGTDGMVHISQM
                     ADHRVEKVTDVVKEGDVVRVKLNSIDERGRLSLSMKDAK"
     gene            1982..2563
                     /locus_tag="VFG56_01980"
     CDS             1982..2563
                     /locus_tag="VFG56_01980"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6622680.1"
                     /translation="MPSKLDLLGQIRADILEKNICPNLAETANNLVMGDGNSDADIVF
                     IGEAPGKKEDEQGLPFVGAAGKFLNEMLSSIELERGDVFITNIVKYRPPNNRDPSPEE
                     KQAFWPYLARQLEIIEPKVIVTLGRHSMEFFLPDKKISQIHGQPKKLPVKLHDGRQSS
                     WIILPLYHPAAALYNGGMRQTLINDFQVVKGLL"
     gene            2710..4845
                     /locus_tag="VFG56_01985"
     CDS             2710..4845
                     /locus_tag="VFG56_01985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET6622681.1"
                     /translation="MGQRRLKGPQGDDMSKRPNNQKKKSNNTVLNNTQTRRGEVFRAQ
                     KRVSENVNARSSQHIIDIPVNKSKYNGYEGRQFSLADQKRQRPNPNRPSLKVIPIGGL
                     GEMGIGKNMMAIEYDQDIIVIDMGFLFPGADYPGINYIVPDINYLEENKHKIRGVVFT
                     HGHLDHIGAFRHLIHRIPAPVYASRFTIGMLKKNMDESTTGYEPEYHELDPESHERVQ
                     LGDSFSIELVRVNHSIPDATAVVVRTPVGVVVDSGDWRFEDNPVDGKKFDLDRLSEIA
                     AKEGILLFMNESTNCESEGTHTHGELDIKVSMGEVMEKYQNSRLVISCFSSQIHRMQV
                     ILEEAKTHGRKVAFAGYSMIQNLEVALRAGVIKVPKDVVVKMDDIIKLPDGKVTIICT
                     GSQGEFNAVLNRMASGAHKHIKIKGSDIIVFSSNPIPGNEKYVVRTVDGLMREGSDVI
                     QNGKTHLTGVGPLHLSGHGYYDDHIKLITALNPTYYLPIHGEFHMLHHNAELAEKECA
                     IPKSNIFVCDAGDVVELYHDGSAKKTGRVPVGGVMYDDSGAEVSEVVLKDRIHMAHEG
                     MFVVILTVARGTGRLLTSPDIISRGFIYMRDSEELMNLIRQYLKQKAARSFSGRKVDL
                     DVLKKEIKDEITHVLYDQTRRTPIVIPVINEVSVNAGKSSKSNGDRNFKDQAKQSKRT
                     TDQTLAHAQPVDSTRPFSHEPANKHIREV"
     gene            complement(4900..5406)
                     /locus_tag="VFG56_01990"
     CDS             complement(4900..5406)
                     /locus_tag="VFG56_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622682.1"
                     /translation="MPLEMFDGRTGQRLIARLPITKDSYAVVKTDLIDSLKVYHPSDL
                     EQNGYPALSPVLSTVMTLVAPMIRKRQELRRTYDQAYQRIVDREVELESADALESALI
                     QIQLDLERDTLDNTRWMVDRPITCSSIMIGRRLTLHLPKTMFDEAAQVSAGQVQTIEI
                     YNAPTDEE"
     gene            5565..>7293
                     /locus_tag="VFG56_01995"
     CDS             5565..>7293
                     /locus_tag="VFG56_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET6622683.1"
                     /translation="MPKKKKRATRAKRGGRKNNRKPTPQHSLPDGFWPQVTALVLVIL
                     ALLLTLGMMSSGGPLPEATWNLGRYLLGWAAFILPILFIFRAVQIFRAEDNLVPTSVS
                     VATIIFAVLAGGFIQLMSSSPVNLALAEAGNGGGVVGWLVAKATLLLVSAGVGALIVF
                     LILIVTSLFVLSISPDKLLNSLVNLFKRDIAEGDAANAAVAAKVKADEPVSKGEFKLN
                     EGVPTTMSTDESKPASKLSTFAGSIKPDKVAEDQAALTSSGDPDWQAPSLDLLEKKQN
                     PADAGDVKHNAQIIKDTLAEFNIGVDMEGANIGPKVTQYTLRPPSGVKLTRITALETN
                     IALNLAAQALRIEAPIPGQRAVGIEVPNRKAADVRLYGILNSKQWQSAREPLSFAIGK
                     DISGDAVVGELNKMPHLMIAGQTGSGKSVMINTLLSSLLYRNSPADMRLILVDPKQVE
                     MAPYEDIPHLLTPIITEPEKCISALKWAVNEMERRYSLLAEEKLRDIASYNRKKKEEA
                     MPYVVIVIDELADLMMVAARDVEALIVRLAQKARAVGIHLVLATQRPSVNVITGLIKA
                     NIPARIAFTV"
BASE COUNT         1893 a         1772 c         1913 g         1715 t
ORIGIN      
        1 ggcgacacgg tagtacttgg caccgctatg gtcggctcca agccgatctc gggcatggat
       61 tacttcccgt tgtctatcga ctatgaagag aagatgtacg ccgcaggtaa gatttctggt
      121 tcacgcttta tcaagcgcga aggccggccg agcgaagagg ccgtcctaat aggtcgcatt
      181 atcgatcgtc cgattcgacc actgtggcca aaaggttacc ggcaagaagt ccaaggtgtg
      241 gcttcggttt tgtcgatgga tccgaatttc cgaccagata tggttgccat ggtagcagtg
      301 tcggcggcat tatcgcttac cggggcgccg tttgatggtc cggttgccgg actacgggtt
      361 ggtttggatg agaaaggtga cttcaaggcc ttcatgagcc gtgaggacat ggaagacaac
      421 cagcttgacc tagttgttgc cggcaccaaa gctggcgtca tgatggttga ggccggggcg
      481 cgtgaagttt cggaggaaaa actagtagag gctatggatt gggcgcagca caatatgcag
      541 ccagctatca aattgcagca ggaactagtc aagaagctag cgcctaaatc gttggaatat
      601 gagttggttc tgccggatga gaatatccaa aaagaagctg acaagtgggt cgaaggcaag
      661 ctcggtgaag acctgcgcaa accttacccg gagcgcaatg aagttatcgg tatcatgcgt
      721 ggtgaattcc ataaggcctt tgaagagaaa cttggtgagg actatccaga acttaaagct
      781 gagtatgacg aggctttcac caaggcgctg cataacgacg tccgtgatgg tattgtccgt
      841 gacggtatcc gtcccgatgg tcgtgagctg actgaaatcc ggcctttgag ctcagagatc
      901 agtatcttac cgcgagctca tggttccagt atcttcactc gtggcgttac ccaaggcatg
      961 aatatagtca cactggcgcc gctaagcttt gctcaaatgg tcgacactat ggaggtaact
     1021 gatggtgagc gccgctacat gcaccactac aacgcacctg gctatactgt cggcgaagtg
     1081 cgccgcctag gttctccggg tcgtcgtgaa atcggccacg gctacttagc cgagagagct
     1141 cttatcccgg ttttgccgac tgccgaagaa ttcccatatg ccattcgctc ggttactgaa
     1201 atcatgagtc aaaacggttc tacctcgatg gcggcaactt gctcatcctg tctggccttg
     1261 atggacgctg gcgtgcctat aagcaaaccg gtttccggta tcgctatggg tctgatgatg
     1321 gacggcgaca agccttatgt aatgtccgat atcgctgatg ccgaagactt cgccggagat
     1381 atggacttca aagttaccgg taccaaggac ggtgttacgg ctcttcaaat ggatatgaaa
     1441 gtccacggtt taccggtaaa catcctcaaa atagccttgg agcagggtaa gcagggacga
     1501 gctcatatct tagaacatat gctgacgacg ctagccgaac cgcgtaacac gttgagtccg
     1561 tacgccccgc agattgagaa gattaagatc aatcctgaga agataggctc tatcatcggc
     1621 aagggtggcg agactatcaa caagatcact agtgaaaccg gtgcccaagt cgatatcaaa
     1681 gatgacggct tagtgacggt tgcctcgagc gacctcgaat caatcaaaaa agccgtcgac
     1741 tggatcaagt cgctagtcga agagccagaa gtcggtaaag tttatgaagg cccagtggtc
     1801 aaggttatgg aattcggggc ttttgtcaac atcatgcctg gtaccgacgg tatggttcac
     1861 atctcacaga tggccgatca ccgggttgag aaagtcaccg acgtcgtcaa agaaggtgat
     1921 gttgttcgcg tcaagttgaa ctctatcgac gagcgtggtc gcctaagctt aagtatgaag
     1981 gatgccaagt aaactagatt tactcgggca aatcagggca gatatcttag agaaaaatat
     2041 ctgcccgaat ttggccgaaa cggctaataa tttagtcatg ggagatggca actccgacgc
     2101 cgatatcgtt tttatcggtg aggcgccagg taaaaaagaa gacgagcagg gtctgccgtt
     2161 cgtcggcgcc gccggtaagt ttttaaacga gatgcttagc agcatcgagc tggagcgcgg
     2221 tgatgtcttc atcactaaca tcgtcaagta ccgaccgccg aataaccgcg accccagtcc
     2281 tgaagaaaaa caggcttttt ggccttatct ggcccggcag ttggagataa ttgagccgaa
     2341 agtcattgtc acgctaggcc gacattcgat ggagttcttt ttgccggata aaaaaattag
     2401 tcagatccac ggccagccga aaaagttacc agttaaattg catgatggca ggcagtccag
     2461 ctggataatc ctgcctttgt atcatccggc agccgctctg tataacggcg gcatgcgcca
     2521 gactttgatc aatgatttcc aagtggtcaa aggattgcta taggggtgaa ggacttgtca
     2581 aaaccaccta aatttgctag tattaaagac acttggtaag ggcaacggcc aaacagatag
     2641 accgcccaaa gtctctgata taagcaaatt atttatttga aatttataaa aataacgaaa
     2701 ggaaaataaa tgggtcaaag acggctcaag ggtccgcaag gtgacgatat gtccaagcgg
     2761 ccaaacaatc aaaagaaaaa atctaacaat acggttttaa ataacaccca gactcgacgc
     2821 ggtgaggttt ttcgggctca gaaacgggta tcggagaacg ttaacgcgcg ctcgagtcag
     2881 cacatcatcg acatcccggt caacaaatcg aagtacaacg gctacgaagg ccgacagttc
     2941 agcctagctg atcagaagcg ccagcggccc aaccccaacc gcccgagcct aaaggtcata
     3001 cctatcggtg gtttaggtga gatgggtatc ggtaagaata tgatggctat cgaatacgac
     3061 caagacatca tcgtcatcga catgggcttc ttgttcccag gtgctgacta cccaggtatc
     3121 aactacatcg tgcctgacat aaactacctt gaggagaaca agcacaagat ccgtggcgta
     3181 gtcttcactc atggccacct tgatcatatc ggcgcttttc gccacttaat ccaccgtatt
     3241 ccagcaccgg tttatgcttc acgcttcact atcggcatgc ttaagaaaaa catggatgag
     3301 tcaactaccg gctacgagcc ggaataccac gagcttgatc ctgaatcaca cgaacgagtc
     3361 caactgggcg atagcttctc gatcgagtta gtaagggtca accactctat tccggacgcc
     3421 acggcggtag tcgtgcggac accggtaggt gtagtggtgg attctggtga ctggcggttt
     3481 gaggataatc ctgtcgacgg taagaaattc gatctagatc gtttgtctga gattgccgcc
     3541 aaagaaggta tcttgctatt tatgaacgaa tcaaccaact gtgaatcaga aggtacccac
     3601 actcacggtg agctagacat caaggtcagc atgggtgagg tgatggagaa ataccagaac
     3661 tcacgcctag tcatcagttg tttttcttca caaattcacc gtatgcaggt gattttggaa
     3721 gaagccaaaa cccacggccg taaagtagcc tttgccggtt actcgatgat tcagaacctg
     3781 gaagtcgcac tacgggctgg tgttatcaaa gttccaaaag atgttgtcgt taagatggac
     3841 gacatcatca agctaccgga cggtaaagtg actattatct gcacgggaag tcagggtgaa
     3901 ttcaacgccg tgctgaatcg tatggcatct ggtgctcaca agcacataaa aatcaagggt
     3961 tcggatatca tcgtattttc atccaacccg attccgggca atgaaaagta cgtcgtccgg
     4021 actgtcgacg gcttgatgcg tgagggttca gacgttatcc agaacggcaa gacacacctt
     4081 actggcgtcg gtccgctaca cttgtctggc catggctatt acgacgatca catcaagctg
     4141 atcacggctc ttaatccgac ttactactta ccgatccatg gcgagttcca tatgctgcac
     4201 cacaacgctg agttagccga gaaagagtgc gccattccga agagtaacat ctttgtttgt
     4261 gatgccggtg acgtagtcga gctctaccat gatggtagtg ccaaaaaaac cggtcgggta
     4321 ccagttggcg gcgtgatgta cgacgattct ggcgctgaag tcagtgaagt cgtcctaaaa
     4381 gatcgtatcc acatggccca cgaaggtatg ttcgtagtga tcttgaccgt ggcgcgaggc
     4441 accggtcgtt tattgaccag tcctgacatc atctcgcgtg gatttatcta catgcgtgac
     4501 tccgaagagt tgatgaacct tatccgtcag tatttgaagc aaaaagcagc ccgcagcttc
     4561 tctggccgca aggttgatct cgatgttctc aagaaagaaa ttaaagacga gattactcat
     4621 gtgctttatg accaaactag acgaacaccg atagttatac cggtgattaa cgaagttagc
     4681 gttaacgcgg gtaagtcgtc taagtccaac ggtgaccgca atttcaaaga tcaagccaag
     4741 cagtccaagc ggacaaccga tcagacgtta gctcacgctc aaccggttga ttcaactcga
     4801 ccgttttcgc acgagccagc caacaagcac atccgcgaag tctagagtct tggctatata
     4861 aataaattga gccccgcacc gacgagggct cgatttatat cactcctcat cggtcggggc
     4921 attgtatatc tcgatagttt gaacttgacc agccgaaacc tgggctgctt cgtcgaacat
     4981 ggtcttcggc agatgtagtg tcagtctgcg gccaatcatg atcgagctac atgtgatggg
     5041 tctgtcgacc atccatctgg tgttatcgag tgtgtctctc tccaggtcaa gttgtatctg
     5101 aatcagggcg gattctaagg cgtcggctga ttccagttct acctctctgt ctacaatccg
     5161 ctggtaggcc tggtcatagg tacggcgcaa ctcctggcgt ttgcggatca tcggcgcaac
     5221 cagcgtcatg acggtgctaa gaactggcga taaagccggg taaccgttct gctccagatc
     5281 tgatggatgg tagaccttaa gactatcgat caaatccgtc tttacgacgg cgtagctatc
     5341 tttggtaatt ggtaagcggg cgatcagccg ctggccggtc ctgccatcaa acatttcgag
     5401 gggcatagct cggtcctttc ggtggtagct atagctaaca gtctaggtcg agggcttttt
     5461 gaatgcaatc gtaagcgact tgccttttgt agcaatttat gctacaattt aaccaagtct
     5521 agccctaaac gatgcaagaa taagggttat aatgaataac gaatatgcca aaaaagaaaa
     5581 agcgagctac tagagctaaa cgcggcggtc gcaagaacaa ccgcaagcct actccgcagc
     5641 acagtctgcc ggatggtttt tggccacagg taacggccct agtcctagtc atattagcct
     5701 tactgctcac actcggtatg atgtcgagcg gtggtccgtt gccagaggcg acttggaacc
     5761 taggacgcta tctactaggc tgggcagcct ttatattgcc aatcttgttt atttttcggg
     5821 cggtgcagat ttttcgtgcc gaagacaacc tggtgcctac gtcggtctca gtagccacta
     5881 ttatatttgc tgttctggct ggtggcttta tccaattgat gtccagttca ccagtcaatc
     5941 tagcactagc cgaggccggt aatggcggcg gagtagtcgg ttggctggta gccaaggcta
     6001 cactgctgct ggtcagtgcc ggtgtaggtg ctttgatcgt ctttttgatc ctaattgtca
     6061 ccagtttgtt cgtccttagt atctcgccgg ataagctatt aaacagcctg gtcaacttat
     6121 ttaagcgcga catcgctgaa ggcgacgctg ctaatgccgc cgttgccgcc aaggtcaagg
     6181 ccgacgagcc ggttagcaaa ggtgaattca aactcaacga aggtgtgccg actaccatga
     6241 gtacggacga atcgaagcca gctagcaagc tgtcgacttt cgcaggcagt atcaagccgg
     6301 acaaggtagc cgaagatcag gctgctctga ccagctctgg cgatccggat tggcaggcgc
     6361 caagtcttga ccttttggaa aagaagcaaa atccggccga tgccggtgac gtcaaacata
     6421 atgcccagat catcaaggat actctggccg agtttaatat cggcgtcgac atggaaggcg
     6481 ccaatatcgg tccaaaagtt actcagtaca ccctgcgtcc acctagtggt gtcaagctga
     6541 ctcggatcac ggcactggag accaacatcg cccttaactt agctgcccag gcgcttagaa
     6601 tcgaagcgcc gattccggga cagcgagcgg tcggtatcga agtgcctaac cgtaaggcag
     6661 ccgacgtccg cctgtacggt atcctgaact ccaagcagtg gcagtcggct cgcgaacctt
     6721 taagcttcgc tatcggtaaa gatatctccg gcgacgcagt ggtgggtgaa ctaaacaaga
     6781 tgccgcactt gatgatagcc ggtcagactg gctcgggtaa gtcggttatg atcaacacgc
     6841 tgctatcgag cttgctttac cgcaacagcc cggctgatat gcggttgatc ctagtcgatc
     6901 ctaagcaggt tgagatggcg ccatacgaag atattccaca tctactcacg cccatcatca
     6961 ccgagccgga gaagtgtatc agcgcactca aatgggccgt taatgagatg gagcggcgct
     7021 acagtttact agccgaagag aaattgcgag atatagcctc ttataaccgc aagaaaaaag
     7081 aagaagccat gccttatgtt gtcatcgtca tcgacgagct agccgatttg atgatggtag
     7141 cagctcgtga tgtcgaggct ctgatcgtac gcctcgctca aaaagcccgc gctgtcggca
     7201 tacatctggt actggctact cagcggccta gtgtcaacgt catcaccggc ttgatcaaag
     7261 ccaacatccc ggctcggata gcttttacgg tgg
//
LOCUS       DASSBW010000068         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_949428, whole genome shotgun sequence.
ACCESSION   DASSBW010000068 DASSBW010000000
VERSION     DASSBW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949428"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VFG56_02000"
     CDS             <1..1068
                     /locus_tag="VFG56_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HET6622684.1"
                     /translation="SAGGAAGGRPVARSTSGASTMGGSLERQDPQTAELRGDGTSPTT
                     RSGDAYRRADEPLAGPLSRHLTNPGVSSSGSRFGEDEIASARNQVEKLTEKRQGVEET
                     AETSGMDYGLTDQADELLERRLKREPLAYILGYKEFYGRDFKVTPDVLIPRPDTETMI
                     ELLSSLLDSGDSSGGGATFRHDRFKLALQGSATDESANLLTLIDIGTGSGAIAITAKL
                     EFPSLQVSASDISPAALRIAMSNAKKLGADIEFLESDLLSNVQRPASIICANLPYVDE
                     AWQTSPETRFEPDMALFAPDGGLKLISELVFQATERQSCGDFMILEADPRQHHSIIEL
                     AHRHDYDWYESRDFMVVLQKN"
     gene            complement(1065..2291)
                     /locus_tag="VFG56_02005"
     CDS             complement(1065..2291)
                     /locus_tag="VFG56_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6622685.1"
                     /translation="MVKERSKTDEATDINQVENQSVDDKATVDSQTKTDRPKHSHPTW
                     FYGGLIFFCFVASFLGAWAFLTSGLIDISKTITSNQQTVVSQEGDLIAKVAKDVSPSV
                     VSIITESVNNSGFFPTEETAAGTGMIISEDGYVLTNRHVIGDRTSSVTIVLADGTVKR
                     NVKVIGRDPLNDLAFLKIDDVDDLKPVSLGDSSTLSVGEKVIAIGNALGQYQTTVTSG
                     IISGLGRPIVAGDRGLGGIEQLSNLLQTDAAINPGNSGGPLLTLSGKVIGINTAVAEG
                     AEGIGFAIPINQAKGLIQSVLKTGQVVRAYLGVRYVSVTPVAKDRYDLGVDDGAYIVA
                     GSRSEPGVLPGSPADKAGLKEKDVITAINGQAIDARHPLSDLISQYTVGDTIDVAYSR
                     SGQTHSTKVTLERYSS"
     gene            complement(2309..>2512)
                     /locus_tag="VFG56_02010"
     CDS             complement(2309..>2512)
                     /locus_tag="VFG56_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622686.1"
                     /translation="YTVYDQVKIPEVAVITSLLGFLAIKIYETARSDTKRWMTELRWP
                     TVFVVFVIMTLLILFNGLDPTQL"
BASE COUNT          632 a          648 c          644 g          588 t
ORIGIN      
        1 agcgcgggtg gcgcggcagg agggcgccca gtcgcgcgca gcacctctgg tgcgagcacg
       61 atgggaggaa gcctggagcg acaggacccg cagacagcgg agctacgtgg cgacggaacg
      121 tcgccgacga cgcgaagcgg agatgcctat agacgagctg acgaaccatt agccggccca
      181 ctttcgaggc atttgacgaa ccctggagtt tcttcgtcag gttcacgatt tggcgaggac
      241 gagatcgcct ccgcccggaa ccaagtcgag aaactgacgg agaaacgaca aggcgttgag
      301 gaaaccgctg agacaagtgg gatggattat ggtttgacag accaagctga cgaattgctc
      361 gagcgacgtc tgaagcgtga gccgctggct tatatactag gttataagga attttatggc
      421 cgggacttca aggttacgcc agacgtcctc atcccccgac ctgataccga aaccatgatc
      481 gagctgttaa gtagcctatt ggactccgga gactcgtctg gcggaggcgc aaccttccgg
      541 cacgaccgct tcaaattggc actacagggg tcagcgacag atgaatccgc caatctcctg
      601 acattaatcg atatcggcac cggctccggc gccatagcca tcacggctaa attagaattt
      661 cctagcctac aggtcagcgc cagcgatatc agtccggcgg ctctaagaat agccatgagc
      721 aatgccaaaa agcttggtgc agatattgaa ttcctggaat ccgatttact gtctaacgtc
      781 cagcgaccag catctataat ctgcgccaat ctgccatatg tcgacgaagc atggcagacg
      841 tcgccggaaa cccggttcga acccgacatg gcactgtttg ctccagacgg tggactaaag
      901 cttatatcag agctggtttt tcaggcaacc gaacgacaat cttgtggcga ctttatgata
      961 ctagaggccg atccaagaca acaccatagc atcatagaac tggcccatcg acatgattat
     1021 gactggtacg agtcaagaga ttttatggtt gttcttcaaa aaaactagct gctataacgc
     1081 tctagagtaa cttttgtgga gtgagtttga ccacttcttg aatacgccac atcgatagtg
     1141 tcgccgactg tgtactggga gatcaaatct gataacgggt ggcgggcgtc aatcgcctgg
     1201 ccgttgatag ccgtgatgac atccttctct ttgagaccgg ctttgtcagc ggggctgccc
     1261 ggcaatacac caggctcaga tcgactgccg gcaacaatgt aagcgccgtc atcaactcct
     1321 aggtcgtagc gatctttagc aaccggtgta accgaaacgt aacgtacacc gagataagca
     1381 cggacaactt gaccggtctt taagaccgac tgaatcagac ccttagcctg attgatcgga
     1441 atagcaaaac caataccttc agcaccttcg gctacagcag tgttgatgcc gataaccttg
     1501 ccgctgagcg tcagtagcgg tccaccggag ttaccgggat taatcgctgc atcggtctgc
     1561 agtaagttgc ttagctgctc aataccccct aagcctcggt cgcctgcgac tatcggccga
     1621 ccgagccctg atatgattcc agaagtcact gtcgtctgat actggcctag cgcgttgcct
     1681 atagcaatga ctttttcacc aacactgaga gtcgatgagt caccgagact aaccggcttt
     1741 aggtcgtcga catcgtctat cttgagaaag gctaggtcat tgagaggatc gcggccgata
     1801 actttgacat tcctcttaac cgtaccgtct gccagtacga tggtaaccga gctagtgcgg
     1861 tcaccaatca catgtcggtt ggtcaggacg tatccgtctt cggagattat catgcccgta
     1921 ccagccgctg tctcttctgt tgggaaaaag ccgctattgt tgacgctctc ggttattata
     1981 gaaaccacac tgggactgac gtctttggct actttagcga taaggtcacc ttcttgactg
     2041 accacggtct gttgattact agtaatggtt ttgctgatat cgatcaagcc gctcgtcaaa
     2101 aaggcccaag caccaagaaa gcttgctaca aaacaaaaga agatcaagcc gccatagaac
     2161 caggtcggat gagaatgttt aggccgatca gtctttgtct gcgaatcgac tgtggctttg
     2221 tcatcaaccg attggttttc aacttgattg atatcggttg cttcgtcggt cttggatcgc
     2281 tctttgacca tgtcgcctcc tttttggttt acagttgagt cggatcgagc ccgttaaaca
     2341 gaattagtag cgtcataatg acgaagacca cgaaaacagt tggccaacgt agttctgtca
     2401 tccagcgctt agtgtcagat cgagctgtct cataaatttt gatggctaag aagccgagca
     2461 gactggttat caccgccact tccggaatct taacctgatc ataaacggta ta
//
LOCUS       DASSBW010000069         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_47360, whole genome shotgun sequence.
ACCESSION   DASSBW010000069 DASSBW010000000
VERSION     DASSBW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47360"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFG56_02015"
     CDS             complement(<1..871)
                     /locus_tag="VFG56_02015"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622687.1"
                     /translation="MQLDTPLEKVKGVGPKSAEQFSLAGLETVEDLIYFLPRDYEDYS
                     NSLSIADIKPGKVTLKGKFSGVTTKRVRRGMHITEATLRDHSDAVAVTWFNQPYRADQ
                     IGKGGEWLVSGEFKLSGQRYQLLNPAVESASGDALKAGHIVPVYRAIRGLKSQLVFKI
                     LSELKPLITMLDETLPPKLVEKQGLISHSEALTKLHFPKSSADVEVARARLGFEEVLS
                     LMLAAELNKQTNARLKSFAIPFDAKLAQKFVASLPFKLTDDQRLSAWEIVQNLEADTP
                     MNRLLQGDVGAGKT"
     gene            complement(876..2090)
                     /gene="tyrS"
                     /locus_tag="VFG56_02020"
     CDS             complement(876..2090)
                     /gene="tyrS"
                     /locus_tag="VFG56_02020"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET6622688.1"
                     /translation="MTLSEELTWRGFKNQTTFKDLASLDEAPLTFYWGVDPSADSMTI
                     GNLAAAMMVRHFINYGHKAVLLVGGATGLIGDPDGKRQERDLKSREEVDRNIEAIAHQ
                     YKTIFAGQSFDIVNNYDWFKGINYLEFLRDVGKHMSMTQLLDREFVQSRIGEGGAGIS
                     YAEFSYSLIQGYDFLHLFRERGVTLQLSGADQWGNSLSGVEMIRKLEAKEAHVYTTPL
                     IVNKTTGAKFGKSEAGAVWLDPNKTSIYQFYQFWLNLGDDGVVDYLKIFTLLSKDAIE
                     DLAKQVESNPGERAAQKALAYEVTKLVHGEKAADMVTSVTEVLFSSADASSMSSEELK
                     LLAAELPSVKSTEVVEALVEAGLSSSRGEARRFVEAGAISVNGQKATLETDLAAPALI
                     RRGKNQFALKTS"
     gene            2218..4860
                     /locus_tag="VFG56_02025"
     CDS             2218..4860
                     /locus_tag="VFG56_02025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET6622689.1"
                     /translation="MSARRKSRAVSLYSNLSSRRKSSKDRKSRRRAEYLATLPKHPVK
                     RAFYRLHPKRFFAFWFSREGAIMALKIFGVGLACMAVLLGSLFFYYRHELNAIGPAQL
                     AKRVQSTVTKYYDRNGVLLWEDKGTGNYTLVVKSDQISPYLKDATVAIEDKDFYHHGG
                     ISIGGIMRAAVVDISGGQTQGGSTLTQQLVKQVFLAEEADERGISGIPRKIKEAILAI
                     EAERMYTKDQILTMYLNESPYGGRRNGAESAAQTYFGKHAKDLNIAESALLASIPQSP
                     SYYDPYYALDHPQAKKDLLARQETTISYMREQGMITKEQAEEAKQVPILDQIKPASDQ
                     FKNVKAPHFVQMVKSELEQKLGKKVVGEGGLTVKTTLDYRVQKVVDEAMDDLFDSSIP
                     EYAGFDNSAITLVDVQTGQILALRGSRDYDYPGYGAVNAATAYIQPGSSIKPEVYAAL
                     FKQRDGQNYGAGSILSDNPLPQSIYTTGNGQSVMNFDNRFEGNITIRRSLAESRNIPA
                     IKAMYIAGRDLTIDTIHDLGDKTYCTQSVDKTVGLAAAIGGCGLKQTEHVNAYATLAR
                     LGKYEPVSSVLQVKNSAGQIIEKWHDEGKQVIDPQIPYIISDILSDDDARSRTFGHNA
                     AGMVVPGVKTATKTGTSNIGSSAKDLWMMSYSPKVALGMWAGNHVPEALNHVYSTILG
                     ETVGDIMEEIHKDIFQPDGTWHPGQWFKKPDGIQKLTVHGHTDLFPSWFNKKQSSNTK
                     TMTFDKVSKKLATDCTPDAARTDVTISYISDPVTKRKTYLSTEGYDPSEEDDVHECSN
                     KGHQPSIGDVKYRRGQLVVTNVSDGLYSIRSVTIKAGGRSVTASSAGGGKYVASWNGD
                     KPDSATVTVYDDLYYSATITDHNL"
BASE COUNT         1226 a         1331 c         1258 g         1171 t
ORIGIN      
        1 ccgtcttacc ggcgccgaca tcaccttgta gcaaccgatt catcggtgta tcggcttcta
       61 ggttttggac aatctcccag gcactcaggc gctgatcgtc ggttagttta aacggcaggc
      121 tagctacgaa cttttgggcc agcttggcgt caaacggaat agcaaaactt ttcagccggg
      181 cgttggtttg cttgttgagt tcggctgcta gcatcaggct taagacctct tcaaagccga
      241 gccgagcccg agccacctca acatcggctg agctttttgg aaagtggagc ttagtcagag
      301 cttcagaatg cgagatcaga ccctgtttct cgacaagctt tggcggcaag gtttcatcga
      361 gcatggtgat gagcggcttt agttcgctga gtatcttgaa gactagttgt gacttcagtc
      421 cgcgtatcgc tcggtaaact ggtacgatat ggccggcttt caaggcatcg ccactagctg
      481 actcgaccgc cgggttgagt aattgatagc gctgacccga caacttgaat tcaccgctga
      541 ctagccattc tcctcccttg cctatttgat cggcccgata cggttgattg aaccaagtta
      601 cggccacggc gtcgctatgg tccctcaggg tcgcttcggt gatgtgcatg ccgcggcgga
      661 cgcgcttggt tgtaacaccg gaaaacttgc ctttaagcgt aactttgccg ggctttatat
      721 cggcgatact aagactgttt gaataatctt cataatcgcg cggcaagaaa tagatcaagt
      781 cctcgaccgt ctcgagaccg gctaggctga actgctcggc tgacttcggt ccaacccctt
      841 tgaccttttc taacggcgta tctagttgca tagacctaac tggttttaag ggcgaattgg
      901 tttttgccgc ggcgaatcag ggcgggggcg gcaagatcgg tctcgagtgt cgccttctgg
      961 ccattgaccg agatagcgcc ggcttcaacg aagcgtcgag cttcacccct ggacgaactt
     1021 agacccgctt caaccaaggc ttcgactacc tcggtggatt taactgacgg caactcagca
     1081 gccaataact taagctcctc ggaactcatc gaactagcat cagctgagct aaacaggact
     1141 tcagtgacac tggtgaccat gtcggccgcc ttttctccat ggactagttt ggtcacttcg
     1201 taggctaagg ctttttgagc agctcgttcg cccggattgg actcgacctg cttggctagg
     1261 tcctcgatag cgtctttgct tagtagcgta aatatcttga ggtaatcaac cacgccgtcg
     1321 tcacctaggt tcaaccaaaa ctgatagaac tgatagatgc tggttttgtt cgggtcaagc
     1381 cagacggcac cagcctctga cttaccaaac ttagctccgg tggttttgtt gacgatcagc
     1441 ggcgtagtgt aaacgtgggc ttccttggcc tcaagttttc gaatcatctc aacgccggat
     1501 agcgagttgc cccactgatc ggcaccggac agctgtaggg tgacgccgcg ctcccggaac
     1561 aaatgcaaga agtcgtagcc ttggataagc gagtagctaa actcagcgta ggatataccg
     1621 gcaccaccct cgccgatacg gctttggacg aattcgcggt ctaacagttg ggtcatcgac
     1681 atgtgcttgc cgacatcgcg caaaaactcc aagtaattaa tacccttgaa ccagtcgtag
     1741 ttgttgacga tgtcgaagct ctggccggca aagatagttt tgtactgatg ggcgatagcc
     1801 tcgatgttgc ggtcgacctc ctcgcggctt tttaggtcac gttcttgcct cttaccatca
     1861 ggatcaccga tcaagccggt tgcaccgccg accagcaaaa cggctttgtg accgtagttt
     1921 ataaaatgac gcaccatcat agcggctgct aggttgccga tagtcataga gtcggccgat
     1981 ggatcgacgc cccagtaaaa agtaagcggc gcttcgtcta agcttgccaa gtctttgaag
     2041 gttgtttgat ttttaaaacc gcgccaggtc agttcttcgg ataatgtcat cgtttaagat
     2101 ctctatttac agattgtacc tctgtcgatt ataacacgcc aaaaacctta agctgattta
     2161 taatcagtgt tacaatattt agtagttgca atggaattta aggggaaaat aaagtagatg
     2221 tccgccaggc gtaaatctcg agctgttagt ttgtattcca atttatcatc ccgtcgtaag
     2281 tccagtaagg atcggaagtc tcgccgtcga gccgaatact tggccacgct gccgaaacat
     2341 cctgtcaaac gggcctttta tcgcttgcac cctaagcgct tttttgcctt ttggttttct
     2401 cgagaaggtg ccatcatggc cctcaaaatc ttcggtgtcg gactggcctg catggcggta
     2461 ctgcttggct cgctgttttt ctactatcgt catgaattaa acgccatcgg cccggcccag
     2521 ctggccaaac gcgtccaatc aaccgttact aagtactatg accgcaatgg cgtgctccta
     2581 tgggaagaca agggtaccgg caactacacc ttggtggtga aatcagatca gatttcaccc
     2641 tacttaaaag acgctaccgt cgccatcgaa gacaaagact tctaccacca cggcggcatc
     2701 agcatcggcg gcatcatgcg agcagccgtc gtcgacatat ccggtggcca aacccaaggt
     2761 ggttcgacat tgactcagca attggtcaaa caagttttcc tggccgaaga ggctgacgaa
     2821 cgcggcatca gtggtatccc gcgaaagatc aaagaggcta tcttggctat cgaagccgag
     2881 cgcatgtata ccaaagatca gatattgact atgtatctga acgaatcccc ttatggtggt
     2941 cgccgtaacg gtgcagaatc agccgctcag acctatttcg gtaagcacgc caaagactta
     3001 aatatcgccg aatcggccct gctggcttcc ataccgcaga gcccgagtta ttatgatccg
     3061 tattatgcgc ttgatcatcc gcaggctaag aaggatctgc tggccaggca agagacgact
     3121 atctcttaca tgcgtgagca aggtatgatc actaaagaac aggccgaaga agccaagcag
     3181 gtacctatct tggatcaaat caaaccggct tccgatcaat tcaagaacgt caaggcaccg
     3241 cacttcgtcc agatggtcaa aagcgaactt gagcaaaagc ttggtaagaa agtcgtcggt
     3301 gaaggtggtc tgacggtcaa gaccacactg gactatcggg tgcaaaaagt tgtcgacgaa
     3361 gccatggatg atctgtttga ctcgtccatc cctgagtacg ccggtttcga caacagcgcc
     3421 ataactctag tcgacgttca gaccggtcag atcttagccc tgcgcggctc gagggattat
     3481 gactacccag gctacggtgc agttaacgcc gccacggctt acatccagcc gggttcatcg
     3541 atcaagcccg aggtctatgc agcgctattt aagcaacgcg acggacagaa ttacggtgct
     3601 ggctccatct tatccgacaa tcctcttcca cagagtatct acactactgg taacggccag
     3661 tcggtgatga acttcgataa tcggttcgaa ggcaatatca ccatccgtcg ttcgttagca
     3721 gaatcaagaa acatccccgc catcaaggct atgtacatag ccggccgtga tttgactatc
     3781 gataccatcc acgacctagg cgataagacc tactgtacgc agagtgtcga caagaccgta
     3841 gggttggcgg cagccatcgg tggctgtggc ctgaagcaaa ccgaacacgt caatgcttac
     3901 gccaccctag ctcgtcttgg taaatacgaa ccagtcagct cggtactgca agtcaaaaac
     3961 agcgccggac aaattatcga aaaatggcac gacgagggca agcaagtcat cgacccgcag
     4021 atcccttata tcatcagcga tatattgagc gatgacgacg cccgttcacg gacatttggt
     4081 cacaacgcgg ccggtatggt cgtgcctggc gtcaagacag ctaccaagac cggtacctcc
     4141 aatatcggct ccagcgccaa agacctttgg atgatgagct acagtccaaa ggtggctctc
     4201 ggtatgtggg ctggtaacca tgtgcccgag gctctaaacc atgtctactc gaccatattg
     4261 ggcgagactg tcggtgacat catggaggag atccacaagg acatcttcca gccggatgga
     4321 acttggcacc ctggccaatg gttcaagaag cccgatggca tccagaaatt gaccgtacat
     4381 ggccacactg acctattccc gtcatggttc aacaagaagc agtccagcaa caccaaaacc
     4441 atgacgttcg acaaggtcag caaaaagcta gccaccgact gtacgccgga tgccgcccgt
     4501 acagatgtga ccatcagcta catcagtgat ccggtaacca aacgaaagac ttacctatcg
     4561 accgagggtt atgacccgtc cgaagaagac gatgtccatg aatgctccaa caaaggccac
     4621 caaccatcga tcggtgacgt taagtaccga cgcggacagc tggtagtcac caacgtgagc
     4681 gacggcctct actctatccg ctcagtcacc atcaaagccg gtggtcgttc ggtgacagcc
     4741 agctcagccg gcggcggcaa atatgtcgct agctggaacg gcgataaacc ggacagtgct
     4801 acagttaccg tctatgacga tctctactat tcggcaacta taaccgacca caatctctag
     4861 cgattagcta gatcgaccaa gctgtcttcg gcttggtcgg ctggtgaacg gccacgccta
     4921 ggtgatcgcg gtgacttagc ttgctttggt gacttggatt tcgtcggagt cttagctcgg
     4981 ggctta
//
LOCUS       DASSBW010000070         4064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1606797, whole genome shotgun sequence.
ACCESSION   DASSBW010000070 DASSBW010000000
VERSION     DASSBW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606797"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFG56_02030"
     CDS             <1..1017
                     /locus_tag="VFG56_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET6622690.1"
                     /translation="AAKLRIKGDKDADVIVQELGTDHPGDIKRFSKYLHPDIAVITAV
                     SDEHMEFFGSLEAVAAEELSIAGFSEFTVVNRDDIDSHFAKLANTHNLTTYGLSQPAE
                     YRLDLAEASPLDGRTGRIICPEWGEVPISLQLIGDHNSKAAAAALAVGAKLGLTSQQA
                     AIGVAKIKPTAGRMQLLRGLEDSILIDDTYNSSPLAARAALETLYKTEAPQRIAILGS
                     MNELGQLSAQAHEALGKHCNASKLDWVVTIGEEAETYLAPAAKNNGCQVRSFDSAIEA
                     GGFVHSVMEPGAVILAKGSQNKVYAEEALKVLLHSTDEEQQLVRQSPAWLSQKQAYFS
                     SLQS"
     gene            1069..2520
                     /locus_tag="VFG56_02035"
     CDS             1069..2520
                     /locus_tag="VFG56_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622691.1"
                     /translation="MAPKQDESTKPTDDGYEPQPVDTLGDSTTPESSDQSIESNEESA
                     SQSDDSPDNQNEAPVTDSDPVDDTDQSPASDTTVTSTSPADETVASDDNQAQSNDSQT
                     TYVNPNNSAAFGGAAAGTGLAKNGVSRRKKLLIGLLAGLVLILAILVGLWFWYNSTSK
                     VMGDAIDQLLSKPITSSQGTFSLADPTGTNGDIKVSGDFSSRQASDKSSEFTMKLRFN
                     GDGTSVEVPLDVIAASNGDLYVKVSQVKQLLADLLKSQGASSADVKQMQTYFSGLISK
                     VDDQWIKVSKDELNGLTSQASSTDQADVSCVQSTLQKFYADSGQQRQIKQAFSDHPFV
                     KVDKKLGNKDISGHSSLGYSLKADENEGKAFAKAIEDSQLVEGLKKCGLSTDDSTSDS
                     ADDSSQQQPTVHMEVWVDRWNHQFTQFVLSVKDKTTDVSFSLQPSFDEPTGKIDIPSD
                     ATTLDQLRTELQQSFSGMMMSQLSSQSTAVTLQ"
     gene            2616..>4064
                     /gene="leuS"
                     /locus_tag="VFG56_02040"
     CDS             2616..>4064
                     /gene="leuS"
                     /locus_tag="VFG56_02040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET6622692.1"
                     /translation="MKRYNPSEIESKWQKIWSDTELYQAAEDSSKAKKYVAGMFPYPS
                     GAGLHVGHVRNYSIVDVIARFYRQQGYNVLNPIGWDTFGLPAENYAIKTGTPPAVSTE
                     ENIKNFKNQFLRLGMSIDWSRELNTTDPEYYRWTQWIFTKLFERDLAYQAERAQWWCP
                     FDKTVLANEQVEAGRCWRCGNQVEKKMMKQWFFKITEYADELLDGIDELDWPEGIKAM
                     QRNWIGKSVGAEIDFGLDFGSEERNQWIDPEGKKSHLTVFTTRADTIFGATYLVLAPE
                     HIWVTLALKHGSVLENTTEVQAYVDQAAKKSDLERQADQKDKTGVRLDGLMAVNPLTG
                     DKIPIFVADYVLTGYGTGAIMAVPAHDERDYDFANKFKLKITDVIEKPADFDDGSYDG
                     EGRLVNSGDYDGTDSAQARELIVADLAKQKLAQEKTNYRMNDWLISRQRYWGAPIPII
                     HCEHDGAVAVPEDDLPVILPSVDKYEPDGSGRG"
BASE COUNT         1101 a         1008 c         1036 g          919 t
ORIGIN      
        1 gcggctaaac tgcgtatcaa aggcgataaa gacgccgacg tcatcgttca ggagctgggc
       61 actgatcacc cgggcgacat caagcggttc tccaaatatc ttcaccccga tattgccgtt
      121 ataaccgccg tcagcgacga acatatggag tttttcggca gcctcgaagc agtcgcggct
      181 gaagaactgt cgatcgccgg atttagcgaa tttacagtag tcaaccgcga tgacatcgat
      241 agccactttg ctaaacttgc caatacccac aatctgacta cctatggatt gagtcagccg
      301 gctgagtacc ggttagattt ggccgaagcc agtccgcttg atggccgtac cggtagaatt
      361 atttgcccgg agtggggtga agtaccgatc agtttgcaat taatcggtga ccataattcc
      421 aaggccgccg ccgccgcgct ggcagtaggc gccaagttgg gtctaactag tcagcaggcc
      481 gccatagggg ttgccaaaat taaaccgact gctggccgga tgcaattact gcgtggtcta
      541 gaagacagta ttttgatcga tgatacctat aactctagcc cgttagcagc tagagcggct
      601 ctcgagactt tatataagac tgaagcaccc cagcgtatcg ctatactggg ttcgatgaac
      661 gaattaggac agttgtcggc gcaagctcac gaagctcttg gcaaacactg taatgccagt
      721 aaattggact gggtggtaac gattggcgaa gaagccgaaa cttacttggc cccagctgcc
      781 aaaaacaatg gctgccaagt ccgttcattt gatagtgcga ttgaggccgg tggtttcgtc
      841 cacagcgtca tggaacctgg cgctgtgatt ttggccaaag gctcacagaa caaagtctac
      901 gccgaagaag ccctcaaagt actcctccat agtaccgacg aggagcagca gctagtccgg
      961 cagtcaccag catggttgag ccaaaaacag gcttattttt cctcacttca gtcgtaatct
     1021 gttaaactta tgtttaagta gtaatttaaa caatctatcg agggtaaaat ggcgccaaaa
     1081 caggatgaat caacaaaacc aactgacgac ggctacgaac cgcagcctgt cgatacccta
     1141 ggtgactcaa ctacaccgga atcatcggat cagtcgattg aatcaaacga agaatcagct
     1201 agtcaatcgg atgactctcc cgataatcaa aatgaagctc cggttaccga tagcgacccg
     1261 gtcgacgata ccgaccagtc tccagctagt gacacgacgg ttacttcgac tagtccggcc
     1321 gacgagactg ttgcttccga cgacaaccag gcgcaatcaa acgactcaca aacaacttac
     1381 gtcaacccca acaatagcgc ggctttcggc ggcgcagctg ccggcactgg tttagcaaaa
     1441 aacggagtta gccgccgtaa gaagctacta atcggtttgc tggctggact ggtcctaata
     1501 ctagctatcc tggtcggact ttggttctgg tacaactcaa ccagcaaggt tatgggtgac
     1561 gctatcgatc aattgctatc caagcctatc actagtagcc aaggcacttt cagccttgcc
     1621 gacccgactg ggaccaacgg tgatatcaag gtcagtggtg acttttctag ccgacaagcc
     1681 agcgataaat catcggaatt tactatgaaa ctaagattca acggcgacgg cactagcgtt
     1741 gaggttccgc ttgacgtgat cgctgccagt aacggcgact tgtacgttaa agtttcccaa
     1801 gtcaagcagc tactggctga tcttttaaaa agccagggcg cttcttcggc cgatgttaag
     1861 caaatgcaga cctacttcag tgggctaatc agtaaggttg acgatcagtg gatcaaggtt
     1921 agcaaggatg agctgaacgg cctgactagc caagctagct caactgatca agctgacgtc
     1981 agctgcgttc agtcgacgct acagaagttt tatgcagact ctggacagca aagacagatc
     2041 aaacaagctt ttagtgacca cccgtttgta aaagtcgaca aaaagcttgg taacaaagat
     2101 atctccggtc atagtagctt gggatactca ctaaaagccg atgagaatga aggcaaggcc
     2161 tttgccaaag ctatcgaaga cagccagctg gtcgaaggtc tcaagaagtg tggtttgtcg
     2221 accgacgact ccacctctga ctcggccgat gactccagcc agcagcaacc aaccgtccac
     2281 atggaagtct gggtcgatcg ctggaaccac caattcactc aattcgttct gagcgttaaa
     2341 gacaagacca ccgatgtatc attcagcctg cagccgtcat ttgacgaacc gactggtaag
     2401 attgatatcc caagtgacgc cactacgctg gatcagctaa ggaccgagct ccagcagtcg
     2461 tttagcggta tgatgatgtc gcaactgtcg agtcaatcga ccgccgttac cttgcagtaa
     2521 aaagcgcgac ccttgttatc tagcagatag cttcgtcgta tgctctgaac taagaggtag
     2581 ttcatgcgcg tactccttgc tatacttaat cagatatgaa acgctacaat ccaagcgaga
     2641 ttgaatcgaa gtggcaaaaa atatggagtg acaccgaact ctaccaagcc gctgaagatt
     2701 cctccaaggc taaaaaatac gttgccggca tgtttccgta cccatctggc gccggtttgc
     2761 acgttggcca tgttcgcaat tattccatcg tcgatgtcat cgcccgtttc tatcgccagc
     2821 agggctacaa tgtcctaaat ccaatcggct gggatacctt tggtttgcca gccgagaact
     2881 atgcaatcaa gaccggtacg ccaccagctg tgtctacaga ggagaacatc aagaacttca
     2941 agaatcagtt cttgcgtctt ggtatgagta tcgattggag tcgggaactc aacactaccg
     3001 atcccgagta ctaccgctgg acacaatgga tatttaccaa gctgtttgag cgcgatttgg
     3061 cttatcaagc cgagcgagct cagtggtggt gtccattcga caagaccgta ctagccaacg
     3121 aacaggttga ggcaggccgg tgttggcgct gcggcaatca agttgagaaa aagatgatga
     3181 agcagtggtt cttcaagatc actgagtatg ccgacgaact gcttgatggt atcgatgaac
     3241 tggattggcc agaaggtatc aaggccatgc agcgcaactg gatcggcaaa agtgtcggtg
     3301 ctgagatcga tttcggcctg gacttcggca gcgaagagcg caatcaatgg atagatccgg
     3361 aaggtaaaaa gtcccacctg acggtattta ctactcgggc cgatactatt ttcggagcta
     3421 cttatctggt gctagcgcca gagcacatct gggtgacgtt agccctcaag catggtagcg
     3481 tgcttgagaa tacgacggaa gttcaagcct acgtcgatca agccgcgaag aagtctgacc
     3541 tggagcgcca agccgatcaa aaagataaga ccggtgttcg actggatggc ctaatggcgg
     3601 tcaatcctct aactggagat aaaattccga tttttgtagc cgactacgta ctgaccggct
     3661 acggtaccgg tgccatcatg gcggtgccag ctcacgacga acgcgattat gacttcgcta
     3721 ataagtttaa gcttaagatt actgacgtga tcgaaaaacc agctgatttt gacgatggtt
     3781 cttatgacgg cgaaggccga ttggtgaatt ccggggacta tgatggcaca gatagcgctc
     3841 aggccaggga gctaatcgtg gctgatttgg cgaagcaaaa actagcccag gaaaagacta
     3901 actaccggat gaacgactgg ctgatttcac gtcagcgcta ctggggggcg ccaattccta
     3961 taattcactg tgagcatgat ggtgccgtag cggtaccaga agatgatttg ccggtcatat
     4021 tgccatcggt tgataagtac gaaccagacg gttcgggccg tggg
//
LOCUS       DASSBW010000071         4788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_812342, whole genome shotgun sequence.
ACCESSION   DASSBW010000071 DASSBW010000000
VERSION     DASSBW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812342"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VFG56_02045"
     CDS             <1..1228
                     /locus_tag="VFG56_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622693.1"
                     /translation="GNPLGGRGNRFKRGVGSGAAFIGGYKSRREFKHNQQDESVKRAH
                     QNAMADQLLDNNSRFARRAAGVAGDEGVERVRATAIETVMRRGNERKENVEKLQGAFA
                     RLSPFAAQAYADSVENGQFSRAKLDQEISRRIADGQINHADAEELERVANFGSAMTPA
                     AMAAATGSLARSGFLQTSHIRNAEKAIRDHKQMSGGEKQIALDQLAGNINDAAKQSGL
                     AQHMRWGVVGGELLQNGQDFDQNEALSQLSAAEQQQYAELTYQAAGNIADMTEEQKLT
                     AGNQAAALIAKGQAEVETRLGFQKLSSAASISRKAYKDGGSEAFAKTMNEDKNFNTDV
                     ILTAAGARGQMSSQDRSALEEQAREVVEARGGVWDEVVNEARQKAGLFSRDSSGPGPT
                     PPTGTQPTNPTPPTGP"
     gene            1232..2158
                     /locus_tag="VFG56_02050"
     CDS             1232..2158
                     /locus_tag="VFG56_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HET6622694.1"
                     /translation="MSEYKVPQDVEAEDKLLGPFSFRQFIYLIVAAIAGFLCWALAQV
                     FIGLLLIPLPIVVFFLILALPLRKDQPMEAYLLAMVQFFLKPHNRYWSPDGDINVVEI
                     TVPHVVEKHLTKDFSGDEASERLAYLANVIDTGGWATRGVNPAMTDDAAMAVAHTEDV
                     LDEDSGVVQSFDDLLSTSSTQRKQAMIDRLRQSIDQSSGKATTAPSQLAEPTTPTSQP
                     SVDSEQPTGPAPKFDPYPNSMRQKVISPTSQTEQAASKPDDESKHKPSPQTVSPDIMR
                     LANNNDLSISAIAHQAHLKDDASDQEVVISLR"
     gene            2189..3061
                     /locus_tag="VFG56_02055"
     CDS             2189..3061
                     /locus_tag="VFG56_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622695.1"
                     /translation="MPPNQQQPVENNQVPVSPTGTQAMPAVPQAPPMQTAGSAATQPT
                     TSAPATQPNPNTTQNSLLLSELRESMAIMKDGTFRAVIACQSINFDLMSNREREGIEY
                     SYQNFLNSLYFPVQILIRSQKVDIGPYLERLSKVRRNQDNMLLGMLMDDYLDFIDALA
                     QEANIMDKDFYIVVPYFPSGDMNAAVANSKSFFTGLFAGNKNQHIKIDKATYDKAKEE
                     LGNRVGAVTSGLFQMGIKSYQLNTKQLAELYYNVYNPDTAVREPLGDFDNFTGTYTQK
                     GQGPAPTPTNRGGA"
     gene            3061..>4788
                     /locus_tag="VFG56_02060"
     CDS             3061..>4788
                     /locus_tag="VFG56_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HET6622696.1"
                     /translation="MAFGKKQPSAVDIAEAQRQREQQEVEQEFLKGVTTLRDLIAPSS
                     LELNSAYFRLGTKYGRTLYIYGYPRQVYTGWMSSIINIDEVIDISMFVYPVESDVVLK
                     NLRKKVGQLEASISLNQERGKVRDPALEAAIQDAEELRDQLQVGAERFFRYGLYLTVY
                     GDSLDELAFVQHKIEAILGQQLVFSKVASNQQEQGLNSTVPQMSDQLQIRRNMNTGAI
                     STSFPFTSADLTQDNGILYGINMHNNGLVIFDRFSLENANMVVFAKSGAGKSFTVKLE
                     ALRSMMMGSDILIIDPENEYQKLAEAVGGSYVRLSLNSSVRINPFDLPKVIDSDDADN
                     ALRANLVTLHGLLRLMLGGSQVAADGTMTAALSPSEEADLDQSLIDTYARAGITSDPL
                     THNSPPPTINDLYETLLHMGGNGPQLAQRLKKYTTGTFAGIFSQQSNIDINNTMVVFN
                     IRDLEDELRPVAMYIVLSHIWNSVRTEQKKRMLIVDEAWQLMKYDDSANFLFSLAKRA
                     RKYYLGLTTITQDVEDFMGSRMGRAIVANSSMQLLLKQSTSAVDVLSDVFKLTDEEKK
                     RLANFTVGQG"
BASE COUNT         1307 a         1298 c         1126 g         1057 t
ORIGIN      
        1 cggcaatcct ctcggtggac gaggcaaccg cttcaagcgc ggtgtaggta gtggcgctgc
       61 ttttattggt ggctataaat ctcgtcgaga atttaaacac aaccagcagg atgaaagtgt
      121 taagcgagcc caccaaaacg ccatggccga ccagctactt gataacaata gtagatttgc
      181 tagaagggcg gctggtgtag ctggagacga aggtgtagaa cgagttcggg ctaccgctat
      241 cgaaactgtt atgagacgag gtaacgaaag gaaagagaat gtcgagaaac ttcaaggcgc
      301 ttttgctcga ctatcaccgt ttgcagccca ggcttatgct gactcagttg aaaacggcca
      361 gttttctcga gccaaacttg atcaagagat tagtcgtcgg atagccgacg gccaaatcaa
      421 ccatgccgat gctgaagaat tagagcgagt tgctaacttt ggctcagcca tgacaccggc
      481 tgctatggct gctgctaccg gctcacttgc taggagtggg ttcctgcaga caagtcatat
      541 tagaaacgcc gaaaaggcta tccgtgatca taagcaaatg tctggaggag aaaagcaaat
      601 tgcgcttgac caactagctg gcaatataaa tgatgccgcc aaacagagtg gcttagccca
      661 acatatgcgc tggggtgtag tgggtggaga gttactacaa aacggtcaag acttcgatca
      721 aaacgaagca ttatcccaac tttcagccgc tgaacaacaa cagtacgctg aacttacata
      781 tcaagctgcc ggcaacattg cagatatgac cgaagagcaa aaactgaccg ctggaaatca
      841 agcagctgct cttatcgcca agggtcaagc tgaagttgaa acacgtcttg gtttccaaaa
      901 actatccagt gctgcatcga tatctcgtaa ggcctacaaa gacggtggct ccgaagcttt
      961 tgctaagact atgaacgaag ataagaactt caacaccgat gtaattctga cggctgccgg
     1021 tgctcgtgga cagatgtcat ctcaggaccg aagtgccctg gaagagcagg ctcgcgaagt
     1081 cgtcgaggct cggggcggtg tttgggatga agttgtcaat gaagcaaggc agaaagccgg
     1141 tttgtttagt cgagattcat caggccctgg acctacccca cctaccggaa cacaaccaac
     1201 taatccaact ccgcccacag ggccttaacc tatgagtgaa tacaaagtac ctcaagacgt
     1261 cgaagccgaa gacaaactac tagggccgtt tagcttccgg cagttcatct atctgatcgt
     1321 cgccgctatc gccggcttct tgtgttgggc gctagcccag gtcttcatcg gtctcttact
     1381 gataccgcta cccatcgtgg tcttcttttt gatcttggca ctgcctctca gaaaagacca
     1441 acctatggaa gcctacttgc ttgccatggt tcagttcttc ctaaaaccac acaaccgcta
     1501 ctggagtccc gatggtgata tcaacgtagt tgagattacc gtcccacacg ttgtcgaaaa
     1561 gcatctgacc aaagatttca gtggtgatga agcttctgaa cgcctggcct accttgctaa
     1621 tgttatcgat actggtggtt gggccactcg tggtgtaaat cccgccatga ccgacgacgc
     1681 cgctatggcc gtggctcaca ccgaagacgt acttgatgaa gattccggtg tagtccaatc
     1741 tttcgacgat ctactaagta ccagctccac ccagcgcaaa caagctatga tcgatagact
     1801 tcggcagagt atcgatcaat cttccggcaa ggctactaca gcccctagcc aattagccga
     1861 accgaccacc ccaactagtc aaccttcagt cgatagcgaa caaccgaccg gtccggcacc
     1921 gaagtttgac ccttatccta actccatgcg ccaaaaggtg atcagcccga ccagccagac
     1981 cgagcaagca gccagtaaac cggacgacga gtctaagcat aagcctagtc ctcagactgt
     2041 ttcacctgat ataatgagac ttgcaaacaa taacgactta tcgattagcg ccatcgctca
     2101 tcaagctcat ctgaaagatg acgcgagcga tcaagaggtg gtcatatcgc ttcgttaggt
     2161 gctaagtagg tggggataaa aagggtagat gccgccaaac caacagcaac cagtcgaaaa
     2221 caaccaagta ccggtctcac cgaccggtac tcaagctatg cctgctgtac cgcaagctcc
     2281 tcccatgcag accgctggct cggccgccac tcagccgact actagcgctc cagctaccca
     2341 gcctaacccc aacaccaccc agaactcact acttctatct gagttacgtg aaagtatggc
     2401 tatcatgaaa gatggtactt tccgggctgt catagcctgt cagtcgatca acttcgacct
     2461 gatgagcaac cgtgagcggg aaggtatcga gtacagctac cagaactttt tgaactcact
     2521 ctacttccca gtccaaatcc tgatccgttc ccaaaaagtc gatatcggcc catacttaga
     2581 gaggctgtca aaagtccgtc gcaaccagga taatatgcta cttggcatgc tgatggacga
     2641 ctacctcgac tttatcgacg ctctggcaca agaggccaac atcatggata aagactttta
     2701 tatcgtagtg ccgtatttcc catcggggga catgaatgcc gctgtagcca acagtaagag
     2761 tttcttcacc ggtctattcg caggtaataa gaatcagcac atcaagatcg acaaagccac
     2821 atacgacaaa gccaaagaag agctgggcaa ccgggtcggc gctgtaacta gtggactctt
     2881 ccagatgggt atcaaatctt atcagctcaa caccaaacag ttagccgaac tatactacaa
     2941 cgtctacaac cccgatactg ccgttcgtga gccactaggt gactttgaca acttcaccgg
     3001 cacctatacc caaaaggggc agggcccagc gccgactcct accaacagag gaggcgcctg
     3061 atggctttcg gcaagaaaca acctagtgct gtagatatcg ccgaggcaca gcgccaacgc
     3121 gaacagcaag aagtcgagca agaattctta aagggtgtta ccacactacg ggatctgatc
     3181 gctccgtcta gcctggaact aaacagtgcc tacttccgcc ttggcaccaa atacggccgg
     3241 actctctaca tctatggtta cccgcgccag gtctataccg gatggatgag ctcgattatt
     3301 aatatcgacg aagttatcga catttcgatg ttcgtctatc cggtggaaag tgacgtagtc
     3361 ctcaagaacc tacgcaaaaa agtaggtcaa ctcgaggcca gtatctcgct caaccaagaa
     3421 cggggtaagg tacgcgaccc agctctggag gctgccatcc aagatgccga ggaactacgt
     3481 gaccaactac aggttggtgc tgaacgattc ttccgttatg gtctttatct gacggtttac
     3541 ggcgactcgc tcgatgaact ggctttcgtc cagcacaaga tcgaggctat cctgggtcag
     3601 caattagtct tctcaaaagt agcctccaac caacaagagc aggggcttaa ctcaaccgtg
     3661 ccacagatgt ccgaccaact acagatccgc cgcaacatga acaccggtgc catatcaact
     3721 tctttcccat tcacttcggc tgacctaacc caggacaatg gcatcctata cggcatcaat
     3781 atgcataaca atggactggt tatctttgat cggtttagtt tagaaaacgc caacatggtg
     3841 gtcttcgcca agtctggtgc gggtaagagc ttcaccgtca aactagaagc tctgcgctcc
     3901 atgatgatgg gttcggatat tctgatcatc gacccggaga acgaatacca gaaactagcc
     3961 gaggccgttg gcggcagcta tgttcgtttg agcctcaact cctcagtccg aatcaacccg
     4021 tttgatctgc ctaaagtcat agactccgat gatgctgaca acgccctccg ggccaatcta
     4081 gtcaccctgc acggactgct ccgattaatg ctgggcggta gtcaggtggc cgcagacggc
     4141 accatgacgg cggctctgtc accaagtgaa gaagctgacc tcgaccagag tctgatcgac
     4201 acctacgccc gagccggtat aacttccgac ccactgacac acaattcacc acccccgacc
     4261 atcaacgacc tttacgagac attacttcat atgggcggca acggacccca gctggcgcag
     4321 cgtcttaaga aatataccac cggaactttc gccggtatct tctctcagca atccaacatc
     4381 gacatcaaca acaccatggt agtattcaat atccgcgacc tggaagacga attgcgaccg
     4441 gtagctatgt atatcgtcct gtctcacatc tggaactcgg tccgaaccga acaaaagaag
     4501 cgtatgttga tcgttgacga agcttggcaa ctgatgaaat acgatgactc ggctaacttc
     4561 ttgttttctc tagctaaacg agctcgtaag tattacctcg gactcaccac catcacacag
     4621 gacgtagaag actttatggg ttcacgcatg ggccgggcga ttgtggctaa ctcctcgatg
     4681 cagcttcttc tcaagcaatc gactagcgcc gttgatgttc tatctgatgt tttcaaactg
     4741 accgatgaag aaaagaagcg tctagccaac ttcacggtcg gtcaaggt
//
LOCUS       DASSBW010000072         7044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_65020, whole genome shotgun sequence.
ACCESSION   DASSBW010000072 DASSBW010000000
VERSION     DASSBW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65020"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..538)
                     /locus_tag="VFG56_02065"
     CDS             complement(56..538)
                     /locus_tag="VFG56_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622697.1"
                     /translation="MAEYYVVAVAALLFVISFVTKRRFGVLGLGLAAGVIIASQFSLW
                     MTHLFNTQAFSFDPFSDHDMAMIILVLAPAVILLAGGPRYQSHKGALLGSAAFVVLAF
                     VLLIEPLSGNTGWLQQNIPDIVKFFSTNQGLIAAFGVILAIGDMFLSHNPHLKLPKKK
                     "
     gene            complement(801..1712)
                     /gene="rimK"
                     /locus_tag="VFG56_02070"
     CDS             complement(801..1712)
                     /gene="rimK"
                     /locus_tag="VFG56_02070"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HET6622698.1"
                     /translation="MKIAILSNGPGNYSTKRLKQVALERGHEVDVIKYRDCYASIERS
                     QPMVWYQGKPLVGYDAIIPRIASSMTRYGSAIVRQMEMQGVYTIASSIAISRSRDKLR
                     SMQLLARAGVGIPKTVVSRGVTDADDLIEHLGGTPVIIKLARGTHGNGVVLADTKKAA
                     KSVLQAFYVMTSDGTNILLQEYIKESAGEDIRAFVVGGKVVASMKRQSLDDDFRSNLH
                     QGGAGQVVKLTDEERKTAIKAAKSMGLTFCGVDMMRSARGPLVLEVNSSPGFGIEQVT
                     KRDVATPIIEYIERNAKRRNRKDKVGA"
     gene            complement(1709..2140)
                     /locus_tag="VFG56_02075"
     CDS             complement(1709..2140)
                     /locus_tag="VFG56_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HET6622699.1"
                     /translation="MSKRLTLGSLESVKLPQFKAEVLAKVDTGAFSGALHCEDIKVER
                     HDEKAVLSFLPLDTDQVFRTQDFREIIVRSAHGHEQVRYLIPVELEVQGVRYQTEVGL
                     TDRTAMKRDMLLGRRFLLENEAIVDVTLTRDIDDEAERIDL"
     gene            complement(2199..3500)
                     /locus_tag="VFG56_02080"
     CDS             complement(2199..3500)
                     /locus_tag="VFG56_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HET6622700.1"
                     /translation="MRRRGTSLILEAIEPSRKSVDAAELRYPNLREALRAFGPMSYLQ
                     RGGRLIKHPLKKMPKPGKLRQELNTAYLLTASYRLQKSLTPVDKIFWSKQFTLASVGL
                     FGRPNPRQVRVLAAAELRYFESLVDELDNHHEFTRAMLHYYRLLAGNAKSTGSLEKRF
                     GKLLVQVRDYFYDRYGSVLAVFDELPANGELEPIELRRYFRRALKQLAAVDPDWVQWQ
                     VVSNQSAGLSVEAPKKQIVIGRYRAPVHAKDVRGLFAHEVLVHAKRAVGGAKLSKALA
                     EGLPDYLEAEEGLGVLAEAALGDGIPSKIKDRYLDIALAMGNYRRRAVNREQLFNVCY
                     VRAVLRSLDRGFDLDLDDLENQVWQHVNRIYRGSLGDKYVAVFTKDIAYYQGFIRMAK
                     YLKVEAKQGRLTSSLDLALSAKFDPTSISDVRQVKSLTKNK"
     gene            complement(3555..4865)
                     /gene="aspS"
                     /locus_tag="VFG56_02085"
     CDS             complement(3555..4865)
                     /gene="aspS"
                     /locus_tag="VFG56_02085"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HET6622701.1"
                     /translation="MRTLNRELKDKIGETVTIAGWLHKKRLLGGLNFINLRDRSGLAQ
                     VLVKDSAEIEKLRSLQVGTVLRVTGTVVADERAPGGAELHNVTLTVDMPVSEESPIEI
                     DKPIPHKSENLDTLFEYRPISLRNLQEQKVFKIRSSLVRYIRDYLYDHEFTEIDTPKL
                     LAGATEGGAEVFKLDYFGKTATLAQSPQFYKQMMVGVFERVFEIGHSYRAEPSATSRH
                     LTELTMLDVEMGFVESHDEVLDLIGDMTMWTLRHVYDQHGDDLKSWQAPELKLPEDGK
                     VPRFTVTEIHDMYSKANKTDTTQEKDLTPDEERWIADYARKNLNSDLVYATNFPAVAG
                     KFYHKFLEDGTVAWADLIFRGLEIATCPLRENNYDKMVEQMKAAGLNPDDDGFRYYLM
                     AFKYGLPVHGGCGFGIDRLVEKTLGLANVKEATLFPRDINRLGP"
     gene            complement(4862..5866)
                     /locus_tag="VFG56_02090"
     CDS             complement(4862..5866)
                     /locus_tag="VFG56_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6622702.1"
                     /translation="MDVLVLIIDIIVWLLLFAVHGVDLSSSSVSLYELGRRTGDGDKL
                     AEAQLTREKLLPRFRTLQRLLELMLLAIASALTIYLMGWVLGVVVTVLIAMQLDWLST
                     RTFLTRLINKYYRPRETALLHILSGQKWLDWFSAKQSSGRQSVGSKDELVHLVEQSKG
                     VFSRDEVIGIKSLAAFADKKVSDIMTPRSVIESIAASDNVGPLVIDQLHQTGHSRFPV
                     YDPDIDHIVGMLYLHDLVDLRSNHKTVRQAMQPRVFYIRENHSLEHALHGFLRTRHHL
                     FIVVNEYRETVGLLSLEDVIEALLGRSIVDEFDEYDNLRKVAEGNPKGNNQPKDEVNL
                     "
     gene            5891..6595
                     /locus_tag="VFG56_02095"
     CDS             5891..6595
                     /locus_tag="VFG56_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET6622703.1"
                     /translation="MTKTRWIIFAVICLLLLGGIAVLANQKRVDVSGIDASAVIKDGD
                     FPDHVVGDADSKVVFIEYGDYQCPICGSVAQPLKDTLQPYIEQGKIAFVFRNYPLTSI
                     HPNTLAAASAAEAAGLQGKYWQMHDKLYESQDAWANAAPDQRQTYFDGYAQDLGLNVD
                     QFRTDMASKQVSRHVARDRALGEKINVTGTPTFVLNGKKLESSINSALQNGDSAELEK
                     LLDQAIKDTGGQPPKQ"
BASE COUNT         1755 a         1845 c         1710 g         1734 t
ORIGIN      
        1 agttcaagtc tcactgggcc ctccaagtaa agagacctgt catccacagg tctttttact
       61 ttttcttggg tagcttcaga tgtggattgt ggctaaggaa catatctcct attgccaaga
      121 taacaccgaa agcggcaatc aaaccttggt tggtactaaa gaacttgact atgtctggaa
      181 tattttgctg taaccagccg gtattacctg acaacggctc gatcaataaa acaaaggcta
      241 gaactacgaa cgcggctgag cccagcagtg cccctttgtg gctctggtaa cgtggaccac
      301 cggccagcaa aatgacggcc ggtgccaata ccagaattat catagccatg tcatgatccg
      361 aaaaaggatc gaagctgaag gcctgggtgt taaacaaatg ggtcatccaa agactgaact
      421 gagacgcgat gataactccg gctgccagtc caagaccaag cacgccaaag cgtcgcttgg
      481 tgacgaagct gattacaaat agcaaagcgg cgacagcgac aacatagtat tcggccatgc
      541 ttaaatcata acaggtatga gacaaagtat gacacgggtc gccacctaag ataactgcga
      601 ccggacgtca gtgcaatttc ctagggcgca aagacacctg gcggttgcag ctgagcgaca
      661 gaagcatttt cttcttcggt gcgtgaacga agtggtacaa gaagtaccgc aaatgagccc
      721 atcggagaaa aaaatgttta tggcgtcagc agcttagcta aagctccggc tttgccgagc
      781 tttgctaagc gatgcaatcg tcaggctccg actttgtcct tgcgattgcg ccgtttggca
      841 ttacgctcga tgtactcaat gatcggcgta gccacgtcac gcttagtcac ctgctcgata
      901 ccgaatcccg gactggagtt gacctccaag accaatgggc cacgagccga acgcatcata
      961 tcaacaccgc agaaagtcag gcccatcgat ttggcggcct tgatggcggt tttgcgctct
     1021 tcatcggtca atttgacgac ctgaccggcg ccaccttgat gtaagttgga tcgaaaatcg
     1081 tcgtctaggc tttgtcgctt catgcttgcg acgaccttgc cgccaaccac aaaggctcgt
     1141 atgtcttcac cagccgattc cttgatgtat tcctgcaaca agatgttagt accatcggac
     1201 gtcatgacgt agaaagcttg gagaaccgac ttagcggctt ttttggtatc agccagaact
     1261 acgccattac cgtgtgtacc acgagccagc ttgatgatga ccggggtgcc gcctaggtgc
     1321 tctatcaaat catcagcatc agtcacgccg cgagacacca cagtcttagg gataccgaca
     1381 ccggctcgag ccagaagctg catggagcgc agcttgtcac gtgagcgcga aatagcgata
     1441 gagctggcga tagtgtagac gccttgcatc tccatttgcc ggacgatagc cgagccatag
     1501 cgagtcatcg aagaagcaat ccgagggatg atggcatcgt aaccgacaag tggtttacct
     1561 tggtaccaaa ccatcggttg actgcgttcg attgaagcgt aacaatcacg gtatttgatg
     1621 acgtcgactt catgaccgcg ctccaaagca acttgtttta gccgcttggt cgaatagttg
     1681 cccgggccgt tagataaaat agcaattttc atagatcgat cctttccgct tcgtcgtcga
     1741 tgtcgcgtgt tagcgtgaca tcgacaatag cttcgttctc caaaagaaaa cgtcgcccta
     1801 gcagcatatc acgcttcatc gccgtgcggt cggtcaggcc gacttcagtt tggtaacgta
     1861 cgccttggac ctccagctcg accggtataa gatagcggac ttgttcatgc ccatgggccg
     1921 aacgaacgat tatctcgcga aaatcttgag ttctaaatac ctggtcggta tctaaaggaa
     1981 gaaacgacag tacggctttt tcatcgtgac gctcgacctt gatatcctcg cagtgcagcg
     2041 caccagaaaa agcaccggta tcgactttgg ccaaaacttc agccttaaac tgcggtaatt
     2101 taacgctctc taagctaccc aatgtcaatc gtttgctcat gcttgatatt atgcctattt
     2161 atctacgtta attctatagc tcggcgtatt atacaatttt acttattttt agtcaatgac
     2221 ttaacctgcc gtacgtcact gatactggtc ggatcaaact tagcactcaa agctaggtcc
     2281 aagctactgg tcagcctgcc ttgttttgct tcaaccttaa ggtacttagc catcctgata
     2341 aagccttgat agtacgcgat gtctttggtg aaaaccgcta cgtatttgtc gcctagacta
     2401 ccgcgataaa tccgattgac gtgctgccaa acctggtttt ccaagtcatc caaatccaga
     2461 tcaaagccac gatccaagct tcttagtact gccctgacgt aacagacatt aaataactgt
     2521 tcgcggttaa cggcgcggcg acggtaatta cccatagcca gagcgatgtc taggtaacgg
     2581 tctttgatct tgcttggtat accatcgccc aaagcggctt ccgctaagac acctaagcct
     2641 tcttcagcct ctagataatc cggtaatcct tcggccaatg ccttggataa cttagcgcca
     2701 ccgaccgctc gtttggcgtg caccaatact tcgtgcgcaa acagaccacg aacgtctttg
     2761 gcgtggactg gcgcccggta tcggcctatg acaatctgct tcttcggcgc ctctaccgat
     2821 aaaccagccg actggttgct taccacctgc cactggaccc agtctgggtc gaccgcggct
     2881 aactgtttca acgcacggcg aaaataacgc ctaagttcga tcggttccag ttcgccgttt
     2941 gccggcaact catcaaatac cgctaggact gagccataac ggtcgtagaa ataatcacgt
     3001 acctgcacga gcaacttgcc aaatcgcttc tccagactgc cggtcgactt agcgttaccg
     3061 gccagcaacc ggtagtaatg tagcatggcc cgtgtaaatt cgtggtgatt atcaagttcg
     3121 tcaactagtg attcgaaata acgtagttca gcagcggcca gtactctgac ctgcctcgga
     3181 ttgggtcgcc caaacaatcc gaccgacgcc agcgtgaact gcttgctcca aaatatcttg
     3241 tcgaccggcg tcagggattt ttgtagacgg taagaagcag tcaacaaata agctgtgttt
     3301 aactcttgac gaagtttgcc gggtttcggc attttcttga gagggtgttt gattagtcgt
     3361 ccgccgcgct gcagatagct catcggaccg aaagctctta gggcttcacg caaattggga
     3421 tagcgcagct cggccgcgtc aactgacttc cgtgacggtt cgatagcttc taagattagg
     3481 cttgtgccgc gacgtcgcat gtacctttat attttaccat caccagccgg tcgaccggcc
     3541 caaatgatga cgggttatgg gcctaggcga ttgatatcgc gggggaaaag tgtagcttct
     3601 ttgacgttgg ctaggcctag ggttttttcg accaatcgat cgataccgaa accgcaacca
     3661 ccgtgcaccg gcagtccgta cttgaaggcc atcaagtagt agcggaagcc gtcgtcgtcc
     3721 ggattcaagc cggcggcctt catctgctcg accatcttgt cgtagttgtt ttcacgcagc
     3781 ggacaagtag caatctccaa tccacggaag atgaggtcgg cccaagctac cgtaccgtct
     3841 tctaggaact tgtggtagaa cttgccagcc accgccggaa agttagtggc atagaccaaa
     3901 tcagagttta agttcttcct agcataatca gcaatccacc gctcctcgtc cggagtcagg
     3961 tccttttctt gggtagtatc ggtcttgttt gctttggagt acatgtcatg tatctcggtt
     4021 accgtaaagc gcggtacttt gccgtcttcc ggtagtttga gttctggagc ctgccaagat
     4081 ttcaagtcgt caccgtgctg atcgtagaca tgcctgagag tccacatagt catgtcacca
     4141 atcaagtcca atacttcatc gtgcgattca acgaagccca tctcgacatc tagcatagtc
     4201 aactcggtta ggtgccgcga cgtagcactc ggttcggctc ggtagctatg gccgatttca
     4261 aatacccgct caaatacgcc gaccatcatc tgcttataaa actgcgggct ttgagctaga
     4321 gtcgccgtct taccaaaata atccagcttg aagacctcgg caccgccctc ggtagcgccg
     4381 gctagcaatt tcggagtatc gatctcggtg aactcatgat cgtacaaata atctcggatg
     4441 tagcgaacta gactggaacg gattttgaat accttttgct cctgcaggtt gcgaagagag
     4501 ataggtcggt attcaaatag ggtatcgagg ttttccgatt tgtgtggaat cggtttgtcg
     4561 atctcgattg gtgattcttc gcttaccggc atgtcgactg tcaatgtcac gttatgcagt
     4621 tcggcgccac ctggagctcg ctcgtcagcc actaccgtac cagtgactct tagaaccgtg
     4681 cctacctgta aactacgcag tttttcaatc tcagcagaat ctttgactag cacctgagct
     4741 agaccgctgc ggtcgcgcag gttgatgaag ttcaaccctc ccagtaggcg cttcttgtgg
     4801 agccagccgg cgatggtgac agtctcgcct atcttatctt ttagttcacg gttgagcgtt
     4861 ctcataaatt gacttcgtct ttcggttggt tattgccttt cgggttaccc tcggcgactt
     4921 ttcttaagtt gtcgtattcg tcgaactcgt cgacaatact gcggccgagc agggcctcga
     4981 tgacatcctc taagctaagt aagccgacgg tctcccggta ttcattgact actataaaca
     5041 aatggtgacg ggtacgcaaa aaaccatgca aggcgtgctc tagactgtga ttctcacgta
     5101 tataaaaaac gcgcggctgc atagcttggc gaactgtctt gtgattggac cttaagtcga
     5161 ccaaatcgtg cagataaagc atgccgacga tgtggtcgat atccggatcg tagactggaa
     5221 aacgactatg cccggtttgg tgcagctggt cgatgactag cggcccgaca ttatccgatg
     5281 cggctataga ttcgataacc gaccgaggcg tcatgatgtc tgacactttc ttgtcggcaa
     5341 aggcggccaa ggatttgata ccgatcacct cgtcgcggct aaacacgcct ttggattgct
     5401 cgaccaggtg gaccagctcg tctttcgagc cgacagattg gcggccgctg gattgcttag
     5461 ctgagaacca gtccagccat ttttgtccgc ttaatatatg taataaagcg gtctctctcg
     5521 gccggtagta cttattgata agacgagtca ggaaagtccg cgtactcagc cagtccagct
     5581 gcatggcgat aagcaccgtt acgacaacgc cgagtaccca gcccatcagg tagatggtca
     5641 aggcgctggc gatagctaat aacatcagtt caagcagcct ctgcaaggtg cgaaagcgtg
     5701 gcagcagctt ctcacgggtt aattgtgctt cggccaattt gtcaccatca cctgtccggc
     5761 ggcctaattc gtataagctg acactcgatg agcttagatc aaccccgtgg acggcaaaca
     5821 aaagtaacca gacgatgata tcgatgataa gcactaggac atccattgaa aaccagtata
     5881 atataaatca atgactaaaa cgcgctggat aatcttcgct gtaatctgcc tgctattgct
     5941 cggtggcatc gccgtattgg ccaaccaaaa acgagtcgac gtttcgggta tcgatgcctc
     6001 agccgttatc aaggacggtg actttcctga ccacgttgtc ggtgatgccg attccaaggt
     6061 agtcttcatc gaatacggcg actaccagtg tccgatctgc ggcagtgtcg cccagccgct
     6121 caaagacacg cttcagccct acatcgaaca aggcaagatt gcttttgtct ttcgtaacta
     6181 tccgctaact tcgatccacc ctaacacctt agctgccgcc tcggctgccg aagccgctgg
     6241 tttgcaaggt aaatactggc agatgcacga caagttgtac gagagccaag acgcctgggc
     6301 caacgccgct ccggatcagc ggcagactta cttcgatggc tatgcccagg atctaggctt
     6361 gaatgtcgac cagttcagga ccgacatggc ttctaagcaa gttagccgac atgtcgcccg
     6421 tgaccgagct ctcggcgaga agatcaatgt cacaggtacg ccgactttcg tcttgaacgg
     6481 caaaaaactt gagtcgtcta tcaatagcgc cctgcaaaac ggcgatagcg cagagcttga
     6541 gaaactactc gatcaggcta tcaaagacac cggtggccaa ccgcctaaac aatagaccgc
     6601 ctttaaggac aaataaaaac gcccccgagg gtaagagggg cgtttttaaa tgtttcttta
     6661 tggcttagta gacaatgtag tcttgccact cagccggtag aacttctacc tcaatacgct
     6721 tgagccgtgt aggctcgacg cgaactgcaa tgggcatgta ctatgctcct gtgcgacctc
     6781 gacttatagc caatttatga agctttcgac caagaggcac cttgttttca agcttctggc
     6841 gcgagacaca ggaaccatca tacacccgct gtcaaatgat gtcaacatta aaacgcttgt
     6901 gtctaaaata cgtagggtcg cgcgcttaga ccgatattaa acgagatcaa tgctaggacc
     6961 agacagaaga aaagaattat caaactgttg attaatcttg catagatacc ccgattaacg
     7021 gctactcctg ctagcaatag gcct
//
LOCUS       DASSBW010000073         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_142487, whole genome shotgun sequence.
ACCESSION   DASSBW010000073 DASSBW010000000
VERSION     DASSBW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142487"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFG56_02100"
     CDS             <1..648
                     /locus_tag="VFG56_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622704.1"
                     /translation="ILASDELCQPCSADSAEVWINDKSCQAVIVKSKTAINLTAGTEA
                     DQAIAKSDDRIEYTIYAENTGLKPAKVELTEDLVDVTEYAQLLDNGGGQYDSNKHQLS
                     WGQITLEPGQKLSRSFTVRMLDQIPSSPQGQSEPGSYDCVMTNSFGNTISTKVDCSAA
                     KAVEQTINQLPQTGAGANIIFGTGLLAVVVYFWSRSKQLGREIRLVRKDVNAGVI"
     gene            649..1212
                     /locus_tag="VFG56_02105"
     CDS             649..1212
                     /locus_tag="VFG56_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622705.1"
                     /translation="MNPEKQPLAERGSAEIQAAAEAAAERLKNRQEKSVEQSPDTKAE
                     RVERARHETKEVFAREAGKEKRSGGEPDARTVRRATKRQKQAHYKTTMKHIQSEMPAP
                     ARTFSKVIHNRTVEKTSEAVGATVARPNAILAGSISAFIVVLTVYLITKHYGYVLSGF
                     ETIGSFVIGWTLGLIYDYARVALIRRN"
     gene            complement(1213..4137)
                     /locus_tag="VFG56_02110"
     CDS             complement(1213..4137)
                     /locus_tag="VFG56_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HET6622706.1"
                     /translation="MLNLIITVVVVAAITSVLVSFFYLQYRKVMREAKNYERGLKMVP
                     LLIHIPPLSDDIEAGGRDERDITEESISQAQVMYNIITSTATKGFRSKIYGQRHISLE
                     IVSTRGLIHYYAVVPVVLIDVVKQAIAAAYPSARLEEVEEHNIFNEVSKLSGTIGGEL
                     TLKKEFSYPIATYQEAKRDPMRSILNALSAAGREDGLGIQVLLRPAPEGWTKSVIASA
                     NKIRKDKGKTSGPSGVAPKELLQALWKAPEEKETKPEDKQLTSLEQATVEAMEEKTRH
                     PGYEVLIRIVASSNTATNSQTLLKNVVAAFSLFDSSGKNGFKFSPAKNMETFITSYIF
                     RFFPQTVNRNILNSVELSTIFHLPSQKNIPTSQVQRQGSKQVDGPHQLLDEGFLLGYN
                     VYRGVKKEIRLSTEDRRRHTYIIGQTGTGKSGLLENLALQDMLDGKGFAFVDPHGDSA
                     DRLLAMVPKERVEDVIYFNPGDMENPVGLNLFEFDTPDQRDFLVQEAIAMLYRLYDPQ
                     HQGIIGPRYENWFRNAALTIMSDPAGSSFIDVPQVFNDNDFAKEKLKHVTNQTVLDFW
                     NKEMAQTSDYHKSEVLGWFVSKFGAFLSNEMMRNIIGQTKSGFDLRDIMDNKKILLVN
                     LSKGRTGELNSQLLGMIFVMKFQAAAMGRADVPEDERVDFSLYVDEFQNFATDSFETI
                     LSEARKYRLNLILANQFMTQLTDKIREAVIGNVGTVISGRIGTTDAELMVKKFAPTFD
                     AEDLTRLPNFETITSVMINNVPSSPFSMSLIPPLGRPNPKLYEALRRLSAVKYGRPRA
                     KVEEEIFKRLRAGDEAREQKKRAMRASLEGSNSPASKSSGSSFLDEWLAKRQQLKQTK
                     PKPQPVPTPAPAKPTPPVAKPAPTPAPTKPLTDPASLKPAGAVEVRSPGQVDRSDSEN
                     PEDKLKMEQQLNAAQKAAPAAKPKDSLKVKPSDADEDDEIDEIFIDLRGNLSHKKPED
                     NQ"
     gene            complement(4139..4567)
                     /locus_tag="VFG56_02115"
     CDS             complement(4139..4567)
                     /locus_tag="VFG56_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622707.1"
                     /translation="MEPNFSQPGQSPERHFPTPEISRVPRPEVVQPTPEKPAETREVN
                     TGAGSGDTPPPVQPAARPLPAIQQPAKADNDAQTKSTLDDNPIAAGDDDLIEKEWVDK
                     AKQIISETKDDPYAQEKAVNQLQADYLKKRYGRTVKLSDN"
BASE COUNT         1154 a         1223 c         1177 g         1221 t
ORIGIN      
        1 atcctcgcca gtgatgagct ctgtcagccc tgctcggccg attcagctga agtctggatc
       61 aacgataagt cttgccaagc tgttatcgtc aaatcaaaaa ccgccattaa cttaaccgct
      121 ggcaccgagg ccgatcaagc catagccaaa tcggatgacc gcatagaata tactatttat
      181 gctgaaaaca ccggccttaa gccggccaag gttgagttga ccgaagatct agtcgacgtt
      241 accgaatacg cccagctact cgacaacggc ggtggccagt acgactccaa caaacatcag
      301 ttgtcatggg ggcagataac ccttgagcca ggccagaaac tatcacgtag cttcaccgtt
      361 aggatgttgg atcagattcc aagcagcccc caaggtcaaa gtgaacccgg ctcttacgac
      421 tgcgttatga ccaattcttt tggtaacact atcagtacca aggtcgactg ttcggctgcc
      481 aaggctgtcg aacaaactat caatcaatta ccgcaaaccg gtgctggtgc aaacattata
      541 ttcggtactg gactactggc tgtggtggtc tacttctggt cgcgctccaa gcagcttggc
      601 cgtgaaattc gcctagttag aaaagatgtt aacgcaggag ttatatagat gaatccggaa
      661 aaacaacctc tagccgaacg tggtagcgcc gagatccagg cagccgccga ggccgcagcc
      721 gagcgactca agaatcgtca ggaaaaatca gtcgagcagt caccagacac caaagcagaa
      781 cgggtcgaac gagcgagaca tgaaaccaaa gaagtcttcg cacgggaagc cggcaaagag
      841 aaacgatctg gtggcgagcc agatgcccgt actgtccggc gcgcaactaa aagacagaag
      901 caggctcact acaagactac aatgaagcac atccagtctg agatgccagc tccagccaga
      961 actttctcta aagtaatcca taaccgcact gtcgagaaaa ccagtgaagc agtcggtgcc
     1021 accgtggctc gacctaacgc catcttagct ggcagtatca gcgcttttat cgtagttctc
     1081 accgtctacc tcattaccaa gcactacggc tatgtcctgt ctgggtttga gactatcggc
     1141 agctttgtga tcggctggac gcttggtcta atctacgact acgctagagt agccctgatc
     1201 aggcgcaact gattattggt tatcttccgg tttcttgtgg ctcagattac cgcgtagatc
     1261 gatgaatatc tcatcgatct catcatcctc atccgcgtca gacggcttaa ccttcaagct
     1321 atccttcggc ttggcggcag gagcggcttt ctgagcagcg tttagctgtt gttccatctt
     1381 aagcttatct tctggattct cggagtctga ccggtcaact tggcctggcg accgaacttc
     1441 gactgctccg gcaggcttga gactcgccgg atcagtcagt ggcttggttg gtgccggtgt
     1501 aggcgcgggc tttgccacag gtggcgtcgg cttggcagga gccggtgtcg gcaccggttg
     1561 aggcttgggc ttagtctgct ttaattgctg gcgcttcgct agccattcgt cgaggaaaga
     1621 agagccggac gacttgctgg ccggtgagtt ggatccctcc aggctagccc gcatagcccg
     1681 cttcttttgc tccctagcct catctccggc tcttaggcgc ttgaaaattt cttcctccac
     1741 tttggcccta ggtcggccgt atttgacagc cgagagacgc ctcagggctt cgtagagctt
     1801 agggttagga cgacctagtg gcggaatcag tgacatagag aacggactgg acggtacgtt
     1861 attgatcata acgctggtaa tggtctcaaa gttaggcaaa cgggtcaggt cttcggcatc
     1921 aaaggttggt gcaaacttct taaccattag ctcggcatcg gtggtaccga tcctaccgga
     1981 tatgactgtt ccgacgttac cgatgactgc ttcacggatt ttatcggtca actgagtcat
     2041 gaactggttg gccaggataa ggttcaagcg atactttcga gcctctgaca aaatagtttc
     2101 gaaactgtcg gtggcgaaat tctggaactc atcgacatag agcgagaagt ctaccctctc
     2161 atcctctggc acgtcagctc gccccatggc ggcggcctgg aatttcatta caaaaatcat
     2221 accgagcagt tgagagttta actcacctgt ccgaccctta gacaagttaa ccaacaggat
     2281 cttcttattg tccatgatgt cgcgtaagtc aaagcccgat ttggtttgac cgatgatatt
     2341 tcgcatcatc tcgttggaga gaaaagcacc aaacttcgac acgaaccagc ccaatacttc
     2401 cgacttgtgg tagtcacttg tttgagccat ttccttgttc caaaaatcaa gcaccgtctg
     2461 gttggtgacg tgctttagct tctctttagc gaagtcgttg tcgttaaaca cctgcggcac
     2521 atcaataaaa ctagaaccag cgggatcact catgatagtc agagcggcgt tcctaaacca
     2581 gttctcatag cgcggcccga taataccttg atgctgcggg tcgtatagac ggtaaagcat
     2641 ggctatagct tcctgtacta aaaagtctcg ctgatctggc gtatcgaact caaacaaatt
     2701 aagccccacc ggattctcca tgtcacctgg attaaagtag atgacatctt ccacccgctc
     2761 tttcggaacc atggcaagca accggtcggc tgaatcgcca tgcggatcga caaaagcgaa
     2821 gcccttgcca tcgagcatat cttgcaaggc taggttttca agtaagccgg acttaccggt
     2881 accagtctga ccgatgatgt aagtatgacg acggcggtct tcggtactga ggcgtatctc
     2941 ttttttaaca ccgcgatata cgttgtagcc aagcaggaaa ccctcgtcca gtagctggtg
     3001 cggtccgtcg acttgctttg agccttgccg ttgaacttgg gaggttggga tatttttctg
     3061 gcttggtaaa tgaaatattg ttgaaagttc aacactattt aaaatgttcc ggttgaccgt
     3121 ctgtgggaag aaacgaaaga tatacgaagt gataaaagtt tccatattct tagccggact
     3181 gaacttaaaa ccattctttc cactggagtc aaataacgaa aaagctgcta ccacattttt
     3241 aagtagcgtc tgtgaattag tcgccgtatt cgacgaagcg actatgcgaa tcaaaacttc
     3301 gtacccaggg tgacgcgtct tctcttccat cgcctcgaca gtagcctgtt ctaggctagt
     3361 cagctgctta tcttccggct tagtctcttt ctcctctggc gccttccaga gagcttgcaa
     3421 taattctttt ggcgccacgc cactcggacc actggtctta cccttgtctt tgcgaatctt
     3481 gttagcactg gcgatgacgg acttcgtcca gccttccggc gctggtcgaa gcagaacctg
     3541 gatgcctaac ccatcttctc gaccggccgc cgatagggcg tttaagatcg atctcatggg
     3601 atcacgttta gcctcttggt aagtggcgat tgggtaactg aactcttttt taagtgttag
     3661 ttcgccaccg atagtgccag acagtttact gacttcgttg aagatgttgt gttcttccac
     3721 ctcttctagc cgtgccgatg gatatgctgc ggctattgcc tgttttacaa catcaatcag
     3781 cactactggt acaacagcat agtaatggat caggccgcgt gtgctaacga tctccaaaga
     3841 gatatgccgc tgaccgtaaa ttttactgcg aaatcctttg gtggccgtcg atgtaataat
     3901 gttgtacata acttgagctt gggagataga ctcttcggta atgtcacgct catctcgccc
     3961 cccggcctcg atatcatcac tgagaggtgg aatatggata agtagcggca ccatcttgag
     4021 acctcgctca taattcttag cttcccgcat gactttgcga tactgcaggt aaaagaaaga
     4081 caccaaaact gatgttattg ctgctaccac caccactgta ataatcaggt tgagcatact
     4141 agttatcgct taatttgacc gtcctgccgt accgcttctt taaatagtcg gcctgcagtt
     4201 ggtttacggc tttctcctgg gcatagggat cgtccttggt ttcgctaatg atctgcttgg
     4261 ccttatcgac ccactctttt tcaattagat catcgtcgcc ggctgcaatc gggttgtcgt
     4321 ctagcgttga tttagtctga gcgtcgttat ctgccttagc cggctgctgt atggccggca
     4381 agggacgagc ggccggctgg actggcggtg gagtatcgcc acttcctgcg ccggtgttaa
     4441 cttcacgggt ttcagctggc ttttccggag tcggctgaac tacttccggc cgaggtacgc
     4501 gacttatttc cggagttgga aagtgtcgtt ctggtgactg gccgggttgt gagaaattag
     4561 gctccatatg aggtaaatta taacacaaac agtttgacca caagcggtat aaattagctg
     4621 cgacgaacta cgtaaaacac cgctagtgcc agtagtacta cgactaaagc gatgtcttgt
     4681 aatttaagct gaccgaccga ctgcagtgcg atcaaaagca ccggcccgaa tgccaaaacg
     4741 ctcgagatgt aataagctcg gtgactagcc atacc
//
LOCUS       DASSBW010000074         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1552265, whole genome shotgun sequence.
ACCESSION   DASSBW010000074 DASSBW010000000
VERSION     DASSBW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552265"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFG56_02120"
     CDS             <1..679
                     /locus_tag="VFG56_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622708.1"
                     /translation="VTDERLNGIKQVTAAHEMLHAAYDRLSETERQHVNQLIEAEAKN
                     NNDQTVEDLVKIYAKTEPGERLNELHSIFGSQVGDLSSELEDYYRSYFTDRGQVVEFY
                     RQYHSVFDNIRAKQDQLVAELNQLAEQIKSLEADYAQDVNQLNQDIAAFNARASSGQM
                     SRSEYESERASLKQRQANLKSEAATINQKVDLYNQKRQQLIAINAQAAALNRSIDSNL
                     PPIKGVD"
     gene            679..2028
                     /gene="radA"
                     /locus_tag="VFG56_02125"
     CDS             679..2028
                     /gene="radA"
                     /locus_tag="VFG56_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET6622709.1"
                     /translation="MPRSTTLFVCQQCAARYAKWSGRCDNCGAWNSLVEEVAAPTKKA
                     VVDRSAGRRLETADLKTVSAEADDKRLSTGLKEFDEVLGGGLVPGSVSLLAGQPGIGK
                     STLLMQVTGNLGRTQPVLYVSGEESSAQVAGRAKRLGVAGSAKQLELSASTSADDIAA
                     TIASGDYRVVVVDSMQTLSLAEVASAPGTVSQITNSANIIIRAAKAAKTAVILVGHVT
                     KEGSVAGPKVLEHLVDVVLGFEGDRYGGLKLLRAAKNRYGSTAEVALFDMDDTGLKSV
                     DNPSASLLKERSDADGSVILATMEGNRPLLVEIQALVNPTSFGYPKRTASGFDLNRLN
                     VLVAVLEKRTKLKLSDKDIFINVVGGLKLQDPAADLAVAMAIASASAGRRLDDGLVVF
                     GEIGLGGEVRSVRSLDARVKEAKKLGFKSAIGPQSGKQATFVSSVVDLRQALIKHLN"
     gene            2039..>2734
                     /locus_tag="VFG56_02130"
     CDS             2039..>2734
                     /locus_tag="VFG56_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622710.1"
                     /translation="MDLFLIIVLLVILAETTFITYKRLDLVRLPKASILIDTSALIDG
                     RLVAIARSGLVTAPLIVPKSVVEELQFMADKADHDKRERARFGLDVIDTLKSIDGADL
                     RVVDDRNQNHLEVDAQLVELSKRYKSKLLTIDYNLNKAARAQDVEVVNINELAHALRV
                     VHLPGETAELKLVQPGQERDQAVGYLDDGTMVVVDAAKALINQTVEVTITRVLQTSAG
                     KMMFAKLVNAPAKP"
BASE COUNT          689 a          711 c          709 g          625 t
ORIGIN      
        1 tgtcacggat gagcggttaa atggtatcaa acaggttact gccgctcatg agatgttgca
       61 cgccgcttat gaccgcttga gcgagaccga acgtcaacac gtcaaccagt tgatcgaagc
      121 cgaggccaag aacaacaatg atcaaacagt cgaagattta gtaaaaattt atgccaaaac
      181 cgaaccgggc gagcgtctaa atgagctaca ttctattttt ggttcgcaag tcggtgactt
      241 aagttctgaa ctggaagatt attatcgaag ctactttact gatcgcggac aggtcgttga
      301 attttaccgg cagtaccact cggtttttga caatatcagg gccaaacaag atcagctggt
      361 agccgagctc aaccagctag ctgaacagat caagtcgcta gaagctgact atgctcaaga
      421 tgttaaccag ctaaatcaag acatcgccgc cttcaatgca cgggcttcaa gtggtcaaat
      481 gagccgttcc gaatatgaga gcgagagagc tagtctcaag cagcgccaag ctaatctgaa
      541 gtccgaggcc gccaccatca atcaaaaagt cgacttatac aatcagaagc gccagcaatt
      601 aatcgccatc aatgcccaag ctgccgctct caatcgcagc atcgacagca atttgccgcc
      661 gatcaaagga gttgattgat gccgcgttct acgacgctgt ttgtttgtca gcaatgcgct
      721 gcccgctacg ccaagtggtc ggggcgttgt gacaattgcg gtgcctggaa cagcttggtc
      781 gaagaagtgg ctgcacctac taagaaggcg gtggttgacc gctcagccgg tcggcggcta
      841 gagacagccg atctaaaaac cgtctcagcc gaagctgacg acaaacgact atcgacaggt
      901 cttaaagagt tcgatgaagt cctcggcggt gggttggttc caggtagcgt cagtctgcta
      961 gccggccagc ccggtatcgg caaaagtacc ttgcttatgc aagtcaccgg taacttaggc
     1021 cgaactcaac cggttttata tgttagcggt gaggaatcat ccgctcaagt agccggtcgg
     1081 gctaagcgtc tcggtgtagc cggctcggct aagcagctag aattatcggc tagcactagt
     1141 gctgacgaca tagctgcgac catcgcaagc ggcgactacc gtgtcgtggt cgtcgactct
     1201 atgcagaccc tcagtctagc tgaagtcgcc tcagcgcctg gtaccgtatc gcagattact
     1261 aactcagcca atatcatcat ccgagccgcc aaggcggcca aaactgccgt aatcctggtc
     1321 ggtcatgtta ccaaagaagg ttcagtggcc ggacctaagg tgctcgagca tctagtcgac
     1381 gttgttctcg gcttcgaagg cgatcgctat ggcggcctta agctactccg agctgccaaa
     1441 aaccgctatg gctcaactgc cgaagtagcg cttttcgaca tggatgacac cggtctcaaa
     1501 tcggttgaca acccgtcggc cagcctgctc aaagagcgct ctgacgctga tggttcggtt
     1561 attttggcta ccatggaagg caatcgtcca ttgctcgtcg agattcaggc gcttgtcaat
     1621 ccgaccagct tcggctatcc taaacgtacc gcttcggggt ttgatctaaa ccgcctcaac
     1681 gttctggtag ctgtccttga aaagcgcacc aaactgaaat tatctgataa ggatatcttt
     1741 ataaatgtcg ttggcggcct gaagctacaa gatccggcag cggatttggc agtggccatg
     1801 gccatagcct cggccagtgc tggacgacgc ctagacgatg gcttggttgt cttcggcgaa
     1861 atcggtcttg gcggcgaagt gcgttccgtc agatcactcg atgcccgggt caaagaagcc
     1921 aaaaaactcg gcttcaagtc ggcgattggt ccacagtctg gcaagcaagc cacctttgtc
     1981 agctcggtgg tagatctgcg ccaagctctt atcaaacatt taaactaaag gtttatcaat
     2041 ggatttattt ttaatcatcg ttctgctggt gatactggca gaaaccactt tcatcaccta
     2101 taaaaggctt gatctcgtca gactacccaa ggctagtatt cttatcgata ccagcgcctt
     2161 gatcgatggc cgtttagttg ctatcgcccg tagcggtcta gtcacggcac cgctgatcgt
     2221 gcccaaaagt gtagtcgaag aactgcagtt tatggctgac aaggccgatc acgacaagcg
     2281 cgaacgagct cgcttcggcc tcgatgttat cgataccctg aagtcgattg atggcgccga
     2341 tctgcgagtc gtcgacgatc gtaatcagaa ccatctcgag gtggacgctc agttggttga
     2401 actgtccaaa cgttataagt ccaagctctt gacgatcgac tataacttga ataaagcggc
     2461 tagggcgcaa gacgtcgaag tcgtcaacat aaacgagctg gcccatgctc tacgcgtcgt
     2521 ccatctaccg ggcgagacag ctgaacttaa actagtccag cccggacagg aacgcgacca
     2581 agccgttggt tatcttgatg atggcaccat ggtggtggta gacgccgcta aagccctgat
     2641 caaccagacg gtcgaagtga caatcaccag ggtcttgcag acctcggcag gtaagatgat
     2701 gtttgccaag ttagtcaatg ctccggctaa gccc
//
LOCUS       DASSBW010000075        10944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_343501, whole genome shotgun sequence.
ACCESSION   DASSBW010000075 DASSBW010000000
VERSION     DASSBW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343501"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFG56_02135"
     CDS             complement(<1..947)
                     /locus_tag="VFG56_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET6622711.1"
                     /translation="MTSDEQADRYREDAEQNAQKRAALLGLDYVDSRRIAQTAPLIAN
                     VMAVSDMYKTKIVPLEVNTDNAHYLFAITSATPQSVINKLREDYQNAGKIADFVLISE
                     LGFKEFMNRYDPPKEVVYDDVKLADEGDSTTLDEVSKTLESVSTDKILDYLINQADKL
                     GASDIHIESQRDNVRLRLRVDGMLHPVATISHDKYRVLLGSIATRANISTASTHPQSG
                     HMQQTSVVSPDKLINMRIETVTTAYGIDCVIRLFDFDESLLSIDSLGLDDKRRQAIEE
                     LVAHPHGMLMVVGPTGSGKSTTLYSILNALNNPSRKILTL"
     gene            1001..1489
                     /locus_tag="VFG56_02140"
     CDS             1001..1489
                     /locus_tag="VFG56_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET6622712.1"
                     /translation="MSISLLLHNIRSAHNVGSLIRTADGFGVSQVVCSGYTPYPEIEG
                     DSRLPHIRRKLTDQIHKTALGAERFLPVSHVDEAETVIASFRDKGCLVVGLEQAADSI
                     RLDNFGAQRDILLLLGEEVDGLNQRLVKACDVILEIPMHGQKESFNVSIAGAIAMYQL
                     SR"
     gene            1554..2399
                     /locus_tag="VFG56_02145"
     CDS             1554..2399
                     /locus_tag="VFG56_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6622713.1"
                     /translation="MSHKRSSSTSILHSLRVAGAGLLVLAFLVAQFNGWPSLAQDRSQ
                     VLSYATDMSRLSLLDDTNQQRVKAGLPKLSENSLLDASAQAKAEAMVKQDYWSHVSPD
                     GIEPWQFFNDAGYSYRKAGENLAYGFTSAEGTVTAWMNSPTHRDNLLGNYRDVGFGIA
                     SSSHYQGGRYTVVVAHYGLPAQTAAATTTNQAISPPPVTVGATATINYLDQLSAGNWS
                     AIILVSLSLCLGAAIMFGLTHRRMLSYAVETGEHFAMRHPLFDAGLVVAVLSLIMISR
                     IGSVS"
     gene            complement(2400..6068)
                     /locus_tag="VFG56_02150"
     CDS             complement(2400..6068)
                     /locus_tag="VFG56_02150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6622714.1"
                     /translation="MAEAKTKTKQLGVSDYVHLHNHTHHSLLDGLTKVPALVDAVKEA
                     GMEAVAITDHGTLSGTVEFYKAAKEIGVKPIIGMETYVAARTIADRDPAKDKARYHLI
                     LLAMDQTGYRNLMKLSTVANLEGFYYKARIDHDLLEKYGEGLIVLSGCASSELGEALR
                     VDDYDKAKQVAEWYKSVFGDRYYIEVQDHGHPEHTSKWDEQVKINKHLIKLAEELSIE
                     MVVTCDAHYLKHADSEAHEILLCVQTGAFLADQGRMSLSDFELHVTDPKEIIERWGKQ
                     YPRAVSNSRQIADRCNVELDLGKILIPTFPTPKGEDEKSYLHKLVWRGLAQRYGGKSA
                     DQIEKLTPEEAKKSLGKDIIERADYELGVMDSMGYNGYFLIVQDFINWGKNRGIIFGP
                     GRGSAAGSIVSYALKITEIDPMGYGLMFERFLNPNRISMPDIDIDIQDTRRNEVIEYC
                     VQKYGKERVANIATFGTMAARAAIRDVARVLQVPYAEADRLSKLVPPPVQGRHIPLGT
                     SVREDPDLKKEYESSPSAKQVIDLAIKLEGTIRSHGIHAAGVVIAPDDIVNFTPLEMA
                     QKGVVATQYPMGPVEELGLLKMDFLGLSNLTILKNALRIISKVYKVEIELSDIKNDDD
                     KAFELFRQGDTTGVFQFESAGMKRYMKELQPTEFSDLVAMNALYRPGPMAEIPRFIKG
                     KNDAASVAYLHQSLEPILRDTYGVMVYQEQIINLLQLVAGYTPGEADLVRKAIGKKKR
                     DIMQAEEPKFIAGCKKQGLTERQAEQLWAQIQPFADYSFNKAHATCYAQIAYWTAYLK
                     AHYPAAFMAALMTSDQGDIDRLAIEISECSHMGIKVLSPDINESFHEFSVVPGESQIR
                     FGLDAVKGVGTGAVEEILIAREDGPFKTVADFAGRVGGGKFNRKAWESLIKAGAFDKI
                     ADRSDLLFNLDSILAYSSKLSKQKLSGQTDLFGNLTDSVVVASLNLENAPTKHDDRER
                     LAWERELLGLYLSAHPLDHYQFFLEEKAAPLSSLTPEDDKRKASVGGLISDIRSINTK
                     SGSKMAFVKVEDKSGELEVIIFPKLYESLGDSLVLDQVVLVSGKISSTDRDGNPLGEA
                     KLIADVITPVSQEEIDNFEPTGQKAKPLKARAKKSAPALTVATDQVVEEVSAPAMVVK
                     PVEPKTVYVHVKDPNDHDRLVRLKQSANSYPGEDEIILVLGTDKKTAMRLPFSVDGNG
                     QLTQQIIDIYGETSVVVK"
     gene            complement(6213..6518)
                     /locus_tag="VFG56_02155"
     CDS             complement(6213..6518)
                     /locus_tag="VFG56_02155"
                     /inference="COORDINATES: protein motif:HMM:NF040137.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HET6622715.1"
                     /translation="MTKSSNDSLSKKLSDRWSRFKDGQMKGAQEALLQDVFQDLYRQR
                     GKIYRMNFIRGIFFGLGSVLGGTVVIAVLIWMISWFVDFPLIGQYFKELLQAVQQSR"
     gene            complement(6534..6851)
                     /locus_tag="VFG56_02160"
     CDS             complement(6534..6851)
                     /locus_tag="VFG56_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET6622716.1"
                     /translation="MAKSKFAIGAVIGMAAGMVAGILAAPKSGKETRADLKKRADELK
                     SDAKTKTDETKVKAKLKADELKREGKSRLDEAKSQSDELKDRTKNAVEGAKQGFKKPT
                     DKA"
     gene            complement(6865..7122)
                     /locus_tag="VFG56_02165"
     CDS             complement(6865..7122)
                     /locus_tag="VFG56_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622717.1"
                     /translation="MDAMQILVIILSAFLALFLILAIALVVMLIKVTRQIKKVTSTAQ
                     TAAERLSSVAGTVNKVASPALLAKLLTNYVSNLKKSKSKRK"
     gene            complement(7197..7472)
                     /locus_tag="VFG56_02170"
     CDS             complement(7197..7472)
                     /locus_tag="VFG56_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET6622718.1"
                     /translation="MVDLGGWLSAIIIGGLAGWVASMIMKTNASMGILSNIVVGIVGA
                     ILANWLLPLVGISGTNGFSLWSFIVALIGAIILLFIVGLFMRRGSRT"
     gene            complement(7510..8958)
                     /gene="gatB"
                     /locus_tag="VFG56_02175"
     CDS             complement(7510..8958)
                     /gene="gatB"
                     /locus_tag="VFG56_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET6622719.1"
                     /translation="MTFDEAVKKYQVTIGIECHVQLKTATKLFSGASNDAREADPNTT
                     VSPICFGFPGVLPVLNRSAVELAVLAGLALNSEIAEVSRFERKHYFYPDLPKGYQITQ
                     LEEPIVLGGYVEAPMPNGEAVRVRLHHAHMEEDAGKLTHPAGKDYSLVDLNRAGTPLI
                     EIVSEADMHSPAEARAYAEELHRLMTFAGVTDGDMYHGNMRFDVNLSLSPVSSERLGT
                     RTETKNLNSFKSVERAAEYEVIRQAELLDKGERVVQETRGWDEARGRTVSQRSKEDAH
                     DYRYMPEPDIPPLVVDSSQIKELKARLPKLPADYRTAWNQVKLNPSVINALLTSQAMA
                     EVVEAVRSKQPTATSKVANWFASGLDESTVVDYAKMDTDELVALAELTNSGQLSSTGA
                     KQIFNQLVSQGGRAEAIAKTNNLIQVSDETALGAIVDEVLADPACKQAIDDLKAGQDK
                     AIGFLVGQSMKRSKGSGNPQIFQGLIRQKLGL"
     gene            complement(8962..9378)
                     /locus_tag="VFG56_02180"
     CDS             complement(8962..9378)
                     /locus_tag="VFG56_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622720.1"
                     /translation="MSLFVSLMVAAIVLIGIGLLVIILLTNKPGRHLNQAKYRERWMT
                     VMGGLDDTPSAMALTIIQADKLLDLALKERGATGNTLGERMKSSKDLFSDRNGIWQAH
                     KLRNHIAHDDKIKLDIRKTRRALNSYKKALADVGAL"
     gene            complement(9379..10773)
                     /gene="gatA"
                     /locus_tag="VFG56_02185"
     CDS             complement(9379..10773)
                     /gene="gatA"
                     /locus_tag="VFG56_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET6622721.1"
                     /translation="MSLEKLAAEVAAGRITARQLVEQSLEKVEATKGKRALLAIYKDK
                     ALKRADQIDADVANGKSAGRLAGVPFIAKDNMLTYGGKTTAAAKILDNFEAPFQATAI
                     ERLEAEGAILIGKANLDAFAHGGSTENSAYSPTKHPLDDNKVPGGSSGGSAAAVALDI
                     APFALGTDTGGSIRQPASFTGVVGFKPTYGTVSRYGVVAMASSTDVVGPLTKTAADAD
                     LIMQIIAGHDPKDSVTLVDFYDQLADKKPGKLGLVKQWFDEADAGVKRQIEQAVDKLK
                     DLGWLVEPVDMPLFDQSLAAYYVIVPAEVASNLARYDGVRYGVRAEADNLNDMYRQTR
                     EAGFMPENKRRIMIGNYVLSSGYYDAYYKQAAKVRTLIIQKFDEAFAKYDFLIGAVSA
                     ETAFEMGRYVADPLKMYLSDLMTVPASLAGVPGVSLPVGLSDGMPVGLQVVGPAKSDR
                     SILKLAEQIEKEIN"
BASE COUNT         2571 a         2922 c         2660 g         2791 t
ORIGIN      
        1 agggttagaa tcttgcgact gggattgttt agggcgttca aaatagaata aagcgtagtc
       61 gacttacccg atccggtcgg accaactacc atcagcatgc cgtgcgggtg agctaccaac
      121 tcttctatgg cctggcgacg cttatcatct agaccaaggc tatcgatact aagcaggctc
      181 tcgtcaaagt caaacagccg gatgacacaa tcgataccgt aagccgtcgt cacagtctcg
      241 atccgcatgt tgatcagctt atctggacta accaccgatg tctgctgcat gtggccggac
      301 tgagggtggg tcgaggcggt cgagatatta gcccgggtcg caatcgagcc cagaagtacg
      361 cggtacttat cgtgactgat agtagccact gggtggagca taccgtcgac acgcaagcgc
      421 aggcgcacgt tatcgcgctg gctttcgatg tggatatcgg aagcaccgag cttatcggct
      481 tgattgatta gataatcaag aattttgtcg gtactgaccg attccagggt tttagacact
      541 tcatccagag tggtgctgtc gccttcgtcg gccaatttta cgtcatcgta aacgacttcc
      601 ttgggcggat cgtaacgatt cataaactct ttgaagccaa gttcggagat caagacaaaa
      661 tcagcgatct taccggcgtt ttgataatct tcccttagct tattgataac tgattgcggc
      721 gtggctgatg tgatagcaaa gagatagtgg gcattgtcgg tattcacttc cagaggcact
      781 atctttgtct tatacatgtc gctaacggcc ataacgttgg cgattaaggg tgcggtctga
      841 gcaatccgtc gactatcgac ataatcaagg cccaaaagtg ccgcccgctt ctgggcgttc
      901 tgctcggcat cctcgcggta acgatcggct tgttcgtcgc ttgtcattaa accaattgta
      961 gcagaaagct taaacgttaa cggctgctaa aataaagtga gtgtcaatca gcctgctact
     1021 ccacaatatc cgttcggccc acaatgtcgg ttcgcttatc cggaccgccg atggtttcgg
     1081 cgtgagtcag gtcgtctgta gcggctacac gccttatcct gaaatcgaag gtgactctcg
     1141 gctaccgcat attcgacgca agctaactga tcagatacat aagaccgccc tgggcgccga
     1201 gcgtttccta ccggtcagcc acgtcgatga agctgagacg gtgattgcta gcttccgcga
     1261 caagggttgt ctcgtcgtcg gcctcgagca ggccgcagac tcaattcgtc tagataactt
     1321 cggcgctcag cgtgacatct tactcctact cggtgaagaa gtcgacggcc taaaccaacg
     1381 tctggtcaaa gcatgtgatg tgatcctaga gataccaatg cacggccaaa aagagtcgtt
     1441 caatgtctct atagccggcg ccatcgccat gtaccagctc agtcgctaac ttttaagaag
     1501 ttgcttatgc aattggtcta ccgctataat agacacaagc ggtttagacc aaaatgtcac
     1561 acaaacgttc gtcatcaacc tccatactac atagtctccg cgttgccggc gccggtcttt
     1621 tagtactagc ctttctagtc gctcaattta acggctggcc cagtctggcc caggaccgtt
     1681 cacaagtcct aagctacgct actgatatgt cacggctaag cttgctcgac gacactaacc
     1741 agcagagagt aaaggctggc ctacccaagc ttagcgagaa cagcttactc gatgcttcag
     1801 cccaggccaa agctgaggct atggttaagc aggattactg gtctcacgtc tcacccgacg
     1861 gtatagagcc gtggcaattt tttaacgacg ccggctacag ttaccgcaaa gcaggtgaaa
     1921 acctagccta cggcttcact agcgccgaag gtacggtaac ggcctggatg aatagtccga
     1981 ctcaccggga caatttacta ggcaactacc gcgatgtcgg cttcggtatc gctagtagtt
     2041 ctcactacca aggtggaaga tatacggtgg tagtcgctca ttatggttta ccggcccaaa
     2101 ccgcagctgc tactacgacc aaccaggcaa tcagtccgcc accggtaacg gtcggagcga
     2161 ccgccaccat caattacctc gatcaactta gtgctggtaa ctggtcggca attatcctgg
     2221 tcagcctatc actctgttta ggggccgcca tcatgttcgg cttgacccat cgccgtatgc
     2281 tgagctacgc cgtcgagacc ggtgaacact tcgctatgcg tcacccttta tttgatgctg
     2341 ggctggtagt agcggtgctt agtttgatta tgattagccg aatcggtagc gtcagctgat
     2401 cacttaacga ccacgctggt ttcaccataa atatcgatga tttgctgagt tagctgaccg
     2461 ttgccatcca cactaaacgg taaacgcatg gcagttttct tatcagtacc aagtactagt
     2521 atgatttcgt cttcaccagg ataactattg gccgactgct taagccggac tagtcggtcg
     2581 tgatcattgg gatctttgac gtggacataa accgtcttcg gctccaccgg ctttactacc
     2641 atggctggcg cactgacctc ttcaaccact tgatcggtcg ccaccgtcaa cgctggagcg
     2701 gattttttag cacgagcctt aagcggctta gccttttgcc cggtgggttc aaagttatcg
     2761 atttcttcct ggcttaccgg cgtgatgaca tcggcaatca gcttagcctc acctaacgga
     2821 ttaccgtcac gatcggtcga cgagattttg cccgagacta agacgacctg gtccaacact
     2881 aagctatcgc ctagcgactc gtacagtttc gggaaaatta taacttccag ctcacccgat
     2941 ttatcttcaa ctttaacgaa agccatctta gagccgctct tggtgttgat agagcgtata
     3001 tcactgatca aaccaccgac cgaggctttg cgtttgtcgt cctccggtgt caaactgctg
     3061 agtggtgccg ccttctcttc caggaaaaac tgataatgat cgagtgggtg ggcgcttaag
     3121 tacaggccga gcaattcccg ctcccacgct aaacgctccc tatcatcgtg cttggtcggc
     3181 gcattctcca agttgagcga ggcgactacc accgagtcgg tcagattacc gaacaagtca
     3241 gtctggccgc ttagtttttg tttacttaac ttagacgagt aggctaggat gctatcgagg
     3301 ttaaatagca ggtcggagcg atcggcgatc ttgtcgaaag cccctgcttt gataagcgat
     3361 tcccaggctt tccggttgaa cttaccgcca ccgacacggc cggcaaagtc ggccaccgtc
     3421 ttgaacggcc cgtcttcacg agcgatgaga atctcttcga cggcaccggt gccgacgcct
     3481 ttgacggcgt ctaggccaaa tctgatctgc gattcacccg gtacgactga gaattcatgg
     3541 aaactctcgt tgatatcggg cgatagtacc ttgataccca tatgcgaaca ctcgctgatt
     3601 tcgatggcca gccgatcaat gtcgccctgg tcgctagtca ttaaggccgc catgaaagcc
     3661 gctggataat gggctttcaa ataagccgtc caataagcga tctgagcata gcaggtggcg
     3721 tgagccttgt taaaagagta gtcggcaaac ggttggatct gggcccacaa ctgctcggcc
     3781 tgtcgctcgg ttaatccctg tttcttacaa ccggcgataa atttaggctc ttcggcctgc
     3841 atgatgtcac gcttcttctt accgatggct ttccgcacca agtcggcctc acccggcgtg
     3901 taaccggcta ccaattgcag cagattgatg atctgctcct gataaaccat gacaccgtag
     3961 gtgtctctta ggataggctc cagtgactga tgtagataag ctactgaagc agcatcattc
     4021 ttgcccttga tgaaacgggg aatctccgcc atcggtcccg gacggtaaag ggcgttcatc
     4081 gctacaaggt cactaaattc ggttggttga agctctttca tgtagcgctt catgccggcc
     4141 gattcaaatt gaaagacccc ggtggtatca ccttggcgaa atagctcgaa ggctttatcg
     4201 tcgtcgtttt taatatcact aagctcgatc tcaactttat agaccttact gatgatacgc
     4261 agagcgttct tcaagatagt caggttggac agacccaaga agtccatctt aagtaggcct
     4321 agttcctcga ccggccccat cgggtattgc gtggcgacaa cacccttttg agccatctcg
     4381 agaggcgtga aattaacgat atcatccggt gcaatcacca caccggcggc gtgaataccg
     4441 tgcgaccgga tggtaccctc caacttgata gctaggtcga taacttgttt agcgcttggg
     4501 ctagattcgt attctttttt gagatccggg tcttcgcgaa cgcttgtacc caaagggata
     4561 tggcgaccct gaactggcgg tggcaccagt ttacttaaac gatcagcctc agcgtagggc
     4621 acttgtaaga cccgtgccac gtcacgaata gccgctctag ccgccatggt accgaaagta
     4681 gcgatgttag ccacccgctc cttgccgtat ttttgaacgc aatactcgat cacctcgttg
     4741 cgcctagtgt cttgaatgtc gatatcgata tccggcatgg aaatacggtt agggtttagg
     4801 aaccgttcaa acatcaagcc ataacccata gggtcgatct cggtaatctt aagggcgtaa
     4861 gatacgatcg agccagcagc cgaaccgcga cctggaccga aaataatacc gcggttctta
     4921 ccccagttga taaagtcctg gacgattaag aagtagccgt tgtagcccat cgagtccata
     4981 acgccgagct cataatcagc tcgctctatg atatctttgc ccaagctttt tttagcttcc
     5041 tcgggcgtaa gcttttctat ctgatcagca gacttgcccc cgtagcgctg agctaagcca
     5101 cgccagacta gcttgtgcaa gtagcttttt tcatcttcgc ctttgggtgt tggaaaagtc
     5161 ggaatgagga ttttgcctag atcaagttcg acattgcagc gatcagcgat ctgacgcgag
     5221 ttgctcaccg ctctagggta ttgcttgccc cagcgctcga taatttcttt ggggtcggtg
     5281 acgtgtaact caaaatcact caggctcatg cggccttggt ctgccaagaa cgcaccggtc
     5341 tggacacaca gcaggatctc gtgggcttcc gaatcggcgt gcttcaaata atgtgcgtca
     5401 caggtgacca ccatctcaat cgatagctcc tcggccagct tgatcaagtg cttgttgatc
     5461 ttgacctgct catcccactt actggtatgc tctgggtgac catggtcctg tacttcgatg
     5521 tagtagcggt caccaaagac cgatttatac cactcagcca cctgctttgc cttgtcataa
     5581 tcatcaaccc ttagagcttc gcctagttcc gagctggcgc aacccgatag tacaatcaag
     5641 ccttctccgt atttttcgag taagtcgtga tcgatccggg ccttatagta aaaaccttcc
     5701 aggttggcca ccgtcgacag cttcatcaga ttgcggtagc cggtctggtc catggccagt
     5761 aagatcaggt gatagcgggc cttatccttg gctggatcac ggtcagcaat agtccgagca
     5821 gccacgtatg tctccatacc gatgatcggc ttaacaccga tctctttggc ggctttataa
     5881 aactccaccg taccagatag agtgccgtga tcggttatgg ctacggcttc catgccagct
     5941 tctttgacgg catcaaccaa cgccggcact ttagtcaggc cgtcgagcag gctgtgatga
     6001 gtgtgattgt gcaggtggac atagtcacta acacccaact gcttggtctt ggtttttgcc
     6061 tccgccatac accttctatt ataactcaat gagatgcgga gtttcgatga ttcagagaat
     6121 catcataacg ccgctaaaga gagaaatgga agcttgctta catttccgag cgaagcagag
     6181 tttcgactgt tataacagtg ccagagcgga tgttagcgac tttgctggac ggcttgaagc
     6241 aactctttga agtactgacc gattagcgga aaatcaacaa accaagatat catccagatc
     6301 aatacagcta ttactacggt gccgccaagc acgctgccca gtccaaaaaa gataccccgg
     6361 ataaaattca tcctatagat cttaccgcgc tgcctataca gatcctgaaa aacgtcttgc
     6421 agcaaagcct cttgggcgcc cttcatctgg ccatctttaa atctagacca tctgtcgctt
     6481 agctttttgc tcaagctgtc attagaactt tttgtcactg agaagtagcc tgtctaagct
     6541 ttgtccgttg gtttcttgaa accttgtttg gcgccttcta cggcgttctt ggttcgatcc
     6601 ttcaactcgt cgctttgcga cttagcctcg tctagccggc tcttgccttc gcgcttcagc
     6661 tcatccgcct ttagcttggc cttgaccttg gtctcgtcgg tcttagtttt ggcgtcgctt
     6721 ttaagttcgt cagccctctt ctttaggtca gcccgagttt ctttaccact tttcggggct
     6781 gccaatatgc cggccaccat accagcggcc atgccgataa ctgctccgat tgcaaacttt
     6841 gatttagcca tgattgatcc tcctttattt acgcttcgat ttagactttt ttaggtttga
     6901 tacatagtta gtcagtagct tggctagcaa tgccggtgaa gctactttat ttaccgtacc
     6961 ggccacgcta ctcaatcgtt cggcagcggt ttgcgcggtt gaagtaacct ttttgatttg
     7021 tcgcgtcacc ttgatcaaca taacaaccaa agcgatagcg agaattagaa acagtgccaa
     7081 aaaagcgctg aggataatta ccagaatctg catagcgtcc ataacaagcc tttcggatta
     7141 agcagctttt gtctaggagc tattatagca atctgacgga ttagtttaaa gccagcttaa
     7201 gtgcgactac cgcggcgcat aaataagccg acgataaaga gcaggattat ggcaccgatc
     7261 agagccacga tgaaactcca caggctaaag ccattggtac cagatattcc aaccagcggc
     7321 agaagccagt tggcgaggat tgcgccaact ataccgacca cgatgttgga caatatcccc
     7381 atcgaggcgt tagttttcat gatcatagaa gctacccaac cggctagacc accgataata
     7441 attgcgctta accaaccacc tagatctacc atactgacct cctcgattaa gttatggcta
     7501 aattgtagcc tagaggccga gcttttgtcg aatcaggccc tgaaagatct gcggattgcc
     7561 actacctttg gaacgcttca tagactggcc gacgagaaaa ccgatagcct tgtcttgacc
     7621 ggctttcaga tcgtcgatgg cctgtttgca cgccgggtcg gccaaaacct catcgactat
     7681 ggcgccgaga gccgtttcat cgctgacttg gatcaagttg ttggttttgg ctatagcctc
     7741 agctcgaccg ccttggctaa caagttggtt aaagatctgt ttggcgccgg tagagctcag
     7801 ctggccacta ttggttagct cagcgagtgc gactaattca tcggtatcca ttttggcata
     7861 gtcgaccacg gttgattcgt cgagtccgct ggcgaaccag ttagccacct tcgaagtagc
     7921 ggttggctgc ttagaccgga cagcttcgac aacctcggcc atggcttggc tagtcagcag
     7981 ggcgttgatc accgacggat taagcttgac ttgattccat gccgttcgat aatcagctgg
     8041 cagtttcggt aagcgtgcct tgagttcttt tatctggctt gaatcaacta ctagcggcgg
     8101 gatgtccggc tctggcatat agcgataatc atgggcgtct tctttggagc gctgcgacac
     8161 tgtcctgcct ctagcttcgt cccaaccacg ggtttcttga acgactctct cacctttatc
     8221 tagcagttca gcctgacgga tcacctcgta ttcggcggct cgttcaacag acttgaagga
     8281 gtttaagttc ttagtctcgg ttcgagtacc gagccgctca gagctaaccg gactcagcga
     8341 caggttaacg tcaaaacgca tattgccatg gtacatgtcg ccatcggtta cgccggcgaa
     8401 cgtcatcaaa cgatgcagct cttcggcata ggctcgagct tcagccggcg agtgcatgtc
     8461 ggcttcactt acaatctcaa tcaatggagt gccggcgcga tttaagtcga ctaagctgta
     8521 gtctttacca gccgggtggg tcaatttacc ggcatcctcc tccatatgag cgtgatgcag
     8581 acgaactctg acagcttcac cgttcggcat tggcgcctcg acatagccgc ctagaacgat
     8641 aggctcttcc agctgagtga tctgatagcc cttcggcaaa tccggataga aatagtgctt
     8701 acgttcaaat cgactgactt cggcaatctc gctatttagg gccaaaccag ccaacacggc
     8761 tagttcgacg gccgaacggt taagtaccgg caagacacct gggaagccga aacaaatcgg
     8821 gctgaccgtg gtattaggat cagcttcacg cgcgtcatta ctggcaccag agaataactt
     8881 ggtagcggtc tttagctgaa catgacactc gataccgatg gtcacctgat attttttgac
     8941 ggcttcatca aatgtcatgg cttagagcgc tcctacgtcg gctagggcct tcttatatga
     9001 attgagggct cgacgagttt tcctgatatc gagtttgatc ttgtcatcat gagctatatg
     9061 gttgcgcagt ttatgagcct gccagatacc gttgcggtct gaaaataaat ctttcgacga
     9121 cttcatccgc tcacctagtg tattgccggt ggcaccacgt tcttttaaag ctagatcaag
     9181 cagcttatcg gcttggatga tagtcagagc catggcgcta ggcgtatcgt cgagaccgcc
     9241 cataacagtc atccagcgtt cacggtattt agcttgattg agatgacgac ccggtttgtt
     9301 ggtcaataaa atgataacca acaggccgat accgattaaa acaatggcgg ccaccatcag
     9361 gctgacaaac aatgacatct agttaatctc cttctctatc tgttcggcta atttcagaat
     9421 tgaccggtct gatttggccg gaccaacgac ctgcagcccc accggcatgc catcacttaa
     9481 tcctaccggc agactgacgc ccggaacacc agctagcgat gccggcacgg tcatcaagtc
     9541 gcttaagtac atcttgagcg gatcggcgac atagcggccc atctcgaagg ccgtctcggc
     9601 cgatacggcg ccgataagaa agtcatactt agcaaaggct tcatcaaact tctggatgat
     9661 caacgtccgc accttggcgg cctgtttgta gtaagcatcg taataaccac tagacaaaac
     9721 gtagttacca atcatgatcc gccgcttgtt ttccggcata aatccggctt cacgtgtttg
     9781 gcggtacatg tcgttcaggt tatcggcttc agcacggacg ccgtaacgga caccatcgta
     9841 gcgcgccaag ttagaggcta cctcggccgg cacgataacg taataggccg ctaggctttg
     9901 gtcaaatagc ggcatatcaa ccggctcaac taaccagcct agatctttta acttatcgac
     9961 cgcttgttca atctgtcgtt tgacacccgc atcggcctcg tcgaaccatt gcttaaccag
    10021 acctagttta cccggcttct tatcggctaa ttgatcataa aaatcaacca gagtcaccga
    10081 atctttagga tcgtgcccgg cgataatctg catgatcaag tcagcatcgg cagccgtttt
    10141 agtcaacgga ccgaccacgt cggtgctgga tgccatggca accacaccgt agcggctaac
    10201 cgtaccgtaa gtcggcttga agccgactac acctgtaaag ctagctggct gacggataga
    10261 acctccagta tcggtgccga gagcaaacgg cgctatgtcg agagctactg cagcagccga
    10321 accgccagaa ctaccgccag gaaccttatt gtcatctagc gggtgtttgg tcggactata
    10381 ggctgagttc tcggtgctgc caccgtgagc aaaggcatca aggttggctt taccgatgag
    10441 gatggcgcct tcggcttcta gtcgctcgat agctgtagcc tgaaaaggag cttcaaaatt
    10501 gtccaaaatt ttggctgcgg cggtcgtctt acctccgtaa gttagcatgt tgtccttggc
    10561 gataaacggt acgccggcca gtcgtccagc cgacttgccg ttagccacat cggcatcaat
    10621 ttgatcggca cgcttaaggg ccttgtcttt atagatagcc agaagggccc gcttaccttt
    10681 ggtggcctca actttctcca gcgactgctc aactaactgt ctagcggtaa tacggcccgc
    10741 agcaacctcg gcggctaatt tctctagact cactggatca ccctcggtac ttttatctgc
    10801 ccggcctgac tgtccggtgc taacccaagc atcgcttgcg gtgacacgcc atagtcaacc
    10861 gtctcgtcat cacgagttac agtaccggag tagttaactt ggtaggtcgg ctcaacaccc
    10921 tcggtatcga ccgattttag ttgg
//
LOCUS       DASSBW010000076         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_65790, whole genome shotgun sequence.
ACCESSION   DASSBW010000076 DASSBW010000000
VERSION     DASSBW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65790"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFG56_02190"
     CDS             complement(<1..701)
                     /locus_tag="VFG56_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622722.1"
                     /translation="MTRLPTPGGDDGTWGDILNQYLLKSHTADGSLKSGSVTSGSIVN
                     GAVTEAKLDTALQTKINSAGGSGAVSSVNGQTGDVTLTKADVGLGNVDNTSDANKPVS
                     TATQTALDLKMDASQAFALSDLSDIDATVDSATAGQVLKFDGSKWGPGADALGGGSGD
                     PAMGGDLSGTASSAQLVAGSVDTPELADGAITDTKVASGAGIAQSKIANLTTDLAAKI
                     DASEKGTASGVASLDA"
     gene            complement(772..2550)
                     /gene="lepA"
                     /locus_tag="VFG56_02195"
     CDS             complement(772..2550)
                     /gene="lepA"
                     /locus_tag="VFG56_02195"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6622723.1"
                     /translation="MGQSHIRNFCIIAHIDHGKSTLADRMLSLTGTIAERDMKSQMLD
                     VMELEQERGITIKLQPVRMDYKDCQLNLIDTPGHVDFSYEVSRSLAACEGAILVVDAS
                     QGIQAQTLANVYLALEADLTIIPVLNKIDLPAADVEASSLELINLLGCDESDIIKVSA
                     KTGQNIDQVLDAIIERVPAPTGKTEAPTRALIFDSYYDDYRGVVLYVRVVDGSIKRAD
                     QIEMLAAEASGLAVEVGSLSPSMHAATELKTGEIGYIVTNLKTTRQARVGDTVTISRG
                     RSDHALPGYKEVHPFVYAGFFPVSNEDYNNLKDAVDKLALSDSALRYEPENSPVLGFG
                     VRIGFLGLLHLDIIRERLEREYDLELVVTNPSTDYHVNLTNGDELDIKSAADLPEVAS
                     IAEIREPWINGEIVVPKDYVGAVLQLIASKRGRQKNLSYIDERALVSFEAPLANLLTD
                     FYDQLKSVTSGYGSFNYELNDYRAEDLVRLDFYVAGEIVDSLSVMVHRSEAEALGREV
                     VKKLKDVIPRQNFKVALQAAIGGRFIAREDLSAYRKDVTTGLYGGDVSRKKKVLAKQK
                     KGKARMKRFGKVDIPSEAFTVMLKRD"
     gene            complement(2551..>3348)
                     /locus_tag="VFG56_02200"
     CDS             complement(2551..>3348)
                     /locus_tag="VFG56_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET6622724.1"
                     /translation="RMAGGAIRAYQQGLSLHMVPVTLIGVAISTAAFPSMTERLAQNR
                     PDLFKKELQNILRVIIWLALPVSAIAFFTRGYLVHFIFNTGAPVVSGILGILVIAILF
                     RSVYHIASRSFYAQQDTRTPLYISIFSIALNITLAIWFTMGLGAGLFGLAWAQSIVAV
                     VEVAILFLVMSIRIDNLLDPPFLAAILRMFVSTVSMSVITYLSVRTLNLMATDRSFFA
                     TFPKFALIVLISFAAYLGVCRLLKLREANLVIRQLERFALWQPKGNR"
BASE COUNT          850 a          850 c          833 g          815 t
ORIGIN      
        1 gcatctagac tggcaacacc gctagcagtg cccttctcgc tagcatcgat tttggcggcc
       61 agatcagtag tcaggttggc aatcttgctt tgggcaatac cggcaccact agcgactttg
      121 gtgtcagtga tagcgccgtc ggctaactct ggtgtatcga ccgatccagc taccaactgg
      181 gcactggaag ccgtaccgga taagtcgccg cccatggcgg ggtcgccgga gccaccacct
      241 agcgcgtcgg caccgggacc ccatttgcta ccatcaaact tcaaaacttg accagcggta
      301 gccgaatcta ctgtagcgtc tatgtccgac aaatcactta gagcaaaagc ttggctagca
      361 tccatcttga gatctagcgc tgtctgagta gccgtgctaa ccggcttatt ggcgtcggag
      421 gtattgtcga cattgccaag accaacatcg gccttggtca gcgtgacatc gccggtctga
      481 ccattgacgc tgctgaccgc accggagcca ccggctgaat ttatctttgt ctgtaaggct
      541 gtatctagct tagcttcagt aacagcacca ttaacaatag atccgctagt aacactaccg
      601 gacttaagcg agccatcagc ggtgtgtgat tttagaaggt actggttgag gatatcgccc
      661 caagtaccgt cgtcaccacc aggagttgga agtcttgtca tttttttatt tccttgaaga
      721 tgcttctgct tatcagtcta gcagctaaca aaacagtggt caacgagtat tttaatcgcg
      781 ctttagcatg acggtgaaag cctctgatgg aatatctact ttgccgaagc gcttcatccg
      841 tgccttgccc tttttctgct tagccagaac cttctttttc cgcgatacat cgccaccata
      901 caggccagta gtcacatcct tacggtaagc actcagatct tcccgggcaa tgaaacgtcc
      961 accgatagcg gcttgcagtg caactttgaa gttttgccgg gggatgacat ctttcagctt
     1021 cttaacgact tcccgcccga gagcttcagc ttcggaacga tgaaccataa ccgatagcga
     1081 gtcgacgatc tctccggcca cataaaaatc aagtcgaacc agatcctctg cccgataatc
     1141 attgagttca tagttgaagc taccatagcc ggaagtaacc gattttaatt ggtcataaaa
     1201 atctgtcagc agattggcta gcggtgcctc gaagctgacc agagctcgct cgtcgatata
     1261 gctgagattc ttctgacggc cacgttttga agcaatcaat tgcaaaacag cgccgacgta
     1321 atcttttggt acgacgattt cgccattgat ccaaggctcg cgtatctcgg caatagaagc
     1381 tacttcgggt aagtcagcgg ccgatttgat atctagctcg tcgccattgg ttagattgac
     1441 gtgatagtca gtgctaggat tggtgacgac cagctcgaga tcgtattcac gctctagccg
     1501 ttcacggatg atatcgaggt gcaacaaccc taggaagcct atgcggacac caaaaccaag
     1561 caccggcgag ttctctggtt catatctgag agctgagtcg gacagggcca atttatcgac
     1621 agcgtctttt aagttgttgt agtcttcgtt gctaaccgga aagaagccgg cgtagacaaa
     1681 tggatgaact tctttgtagc ccggtagtgc gtggtctgaa cgcccacgag atatagtgac
     1741 agtatcacca actctagcct gccgtgtagt ttttaggttg gttacgatat aaccgatttc
     1801 cccggtcttt aactcggtag cagcatgcat cgacggactg aggctaccga cttcgacggc
     1861 taggccgcta gcttcagcgg ctaacatctc gatttgatca gctcttttga tcgaaccatc
     1921 aaccactctg acatagagta cgacgccgcg gtaatcatcg taataggagt cgaagattag
     1981 ggctcgagtc ggcgcctctg tcttgccggt cggtgccgga acccgttcga taatggcatc
     2041 tagaacctga tcgatatttt ggccggtttt ggcgctaact ttgatgatat ctgattcgtc
     2101 acaaccaagc agattgatta actccagtga actagcttcg acatcagccg ccggcaagtc
     2161 gatcttgttg agcaccggga tgatagtcag gtcggcttct agggccagat aaacattggc
     2221 tagcgtttga gcctggatac cttgactggc atcgactacc aagatagcgc cttcgcaagc
     2281 cgccagacta cgggatactt cgtaggaaaa atcaacgtgc cccggtgtgt cgataagatt
     2341 gagctgacag tctttgtaat ccatccgaac cggttgcagt ttgatagtga tgcctctctc
     2401 ctgctccagc tccatcacgt cgagcatctg gctcttcatg tcgcgttcgg caatagtacc
     2461 cgtcagacta agcattcgat cggccaaggt gcttttgccg tggtcgatgt gagcgatgat
     2521 gcagaagttt cggatgtgcg attggcccat ctagcgattg cctttcggct gccacagggc
     2581 gaaacgctcc aattgtctta tgactaagtt ggcttctctt agtttgagta gtcggcaaac
     2641 acccaggtag gccgcgaaac taatcaatac gataagtgca aacttcggga aagtagcgaa
     2701 gaagctacgg tcggtcgcca tcagattgag ggtgcgaacc gacaaatagg tgatgactga
     2761 catcgataca gtcgatacga acatgcgcag tatagcggct aagaacggcg ggtccagtag
     2821 attatcgatt cgtatactca taaccaaaaa taggatcgcc acttctacta cggcaacgat
     2881 cgactgagcc cacgctagac caaacaaacc agcacccaga cccatagtga accagatagc
     2941 cagcgttata ttaagagcga tggaaaaaat cgagatataa agcggcgtcc gggtatcttg
     3001 ttgggcatag aagctacgtg aagctatatg gtagaccgac cggaacaaga tagcgataac
     3061 taagatacct aagatacccg agacaactgg tgcaccggtg ttaaagataa agtggactaa
     3121 atagcctcga gtgaagaagg caatggcgct gaccggcaag gccagccaga tgataaccct
     3181 caagatattt tgtagttctt ttttaaacaa atccggtcgg ttttgagcta gccgttcggt
     3241 catcgacgga aaggctgctg tcgagatagc tacaccaatc aacgtaaccg gcaccatatg
     3301 tagagacaag ccttgctgat aagcacggat cgctccaccg gccatacg
//
LOCUS       DASSBW010000077         4400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_397730, whole genome shotgun sequence.
ACCESSION   DASSBW010000077 DASSBW010000000
VERSION     DASSBW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397730"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..637
                     /gene="rplE"
                     /locus_tag="VFG56_02205"
     CDS             59..637
                     /gene="rplE"
                     /locus_tag="VFG56_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET6622725.1"
                     /translation="MATAKTTTTEPRLKTLYKTTFTKELQDELELKNVNQVPKLEKIV
                     VSVGIGKHKDDKRYHELVVNNLRKITGQQPVDRMARKSIATFKIRAGMNRVGVSVNLR
                     GARMYEFLDRLVNVAMPRIRDFHGVGAKGFDRSGNYNLGIVEQSIFPELSFEDTQVLH
                     GMQITFVVESGSRAGSRLLLEKFGVPFEKGGK"
     gene            637..906
                     /gene="rpsN"
                     /locus_tag="VFG56_02210"
     CDS             637..906
                     /gene="rpsN"
                     /locus_tag="VFG56_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET6622726.1"
                     /translation="MAKKSMIARDKKRQKMIEKYAAKRAELKAAGDLEGLAKLPTNSS
                     PTRWKNRCMESGRPHAYMRKFGLSRIAFREHASKGEIPGVTKGSW"
     gene            906..1307
                     /gene="rpsH"
                     /locus_tag="VFG56_02215"
     CDS             906..1307
                     /gene="rpsH"
                     /locus_tag="VFG56_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET6622727.1"
                     /translation="MSMQSTDPIADMLTRIRNAIMVGQTEVRLPSSKLKVTVAKELKK
                     ANYLHDVKVEKGSPRDTLVITINAAGQNALINHVARVSRPGRRVYSSAADIPRVKNGR
                     GLILVSTSKGVMDGQAAKKARLGGELICEVY"
     gene            1326..1862
                     /gene="rplF"
                     /locus_tag="VFG56_02220"
     CDS             1326..1862
                     /gene="rplF"
                     /locus_tag="VFG56_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET6622728.1"
                     /translation="MSRIGKLPIQIPQGVTITVDSDAVRVQGPKGQLEQFLLPEVKVK
                     VEDGQALVERVDDSKLAKSRHGLVRSLINNMVTGVTEGFSKTLEIKGVGFRLEGGGRE
                     IQMSLGFSHPIKYQAPEGVDLKVDKMNLTVSGIDKQKVGQVAAEIRSFKKPEPYKGKG
                     IRYEGEQIIRKAGKAGAK"
     gene            1862..2215
                     /gene="rplR"
                     /locus_tag="VFG56_02225"
     CDS             1862..2215
                     /gene="rplR"
                     /locus_tag="VFG56_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET6622729.1"
                     /translation="MSQLAKKLLNQSLRKGRVRARVSGSATKPRLTVTMSNLHVSAQL
                     IDDDTGRTLASATTVGTKQTGNMTDKAAYVGSEIAKKAKQAKITKVVFDRNGRAYAKR
                     LKALADAAREKGLEF"
     gene            2217..2762
                     /gene="rpsE"
                     /locus_tag="VFG56_02230"
     CDS             2217..2762
                     /gene="rpsE"
                     /locus_tag="VFG56_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET6622730.1"
                     /translation="MPENTANRQRQRSPRDTRPREDKLFEEQVINIDRVARVVKGGRR
                     FRFKALVVVGDRKSKVGVGVAKGQDVQAAISKATDVAKKSMITVPVKDGTIPHDIEVK
                     FGGARVLLKPAAPGTGIIAGGAIRTIIGVTGINNLLSKRLGSTNKANIAYATIKALDE
                     LVPAEEWLNQPKQSKKAEVKK"
     gene            2762..3229
                     /gene="rplO"
                     /locus_tag="VFG56_02235"
     CDS             2762..3229
                     /gene="rplO"
                     /locus_tag="VFG56_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET6622731.1"
                     /translation="MKYNELTSPSRQGRKRVGRGIAAGGGKTAGRGTKGQNARTGKKL
                     NATFMGGQRALVQAIPKKKGFRSLRAPAQVVYLDQLNDLKATTIDNFALAEAGLISTP
                     HHSVKLIARGDYNGKATVKVQAASASVKQAITKSGGKFEASPVPLKKADKTTK"
     gene            3413..>4400
                     /locus_tag="VFG56_02240"
     CDS             3413..>4400
                     /locus_tag="VFG56_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET6622732.1"
                     /translation="MNWKTIFASLKSADMRKRLLTVLGIVVAYRFIAQIPVPIAEPTQ
                     LRQILQNTFSSSDLGGFLNLLSGGALTSLSVVLVGLSPYITASIITQLLTKAIPKLEE
                     LQRDGGEAGRRKINQWTRIITLPLAIVQSIAFIYLIRQTVLASNTALLGQTTLYEWVL
                     AVTAMTAGAMVMMWLGELITEQGVGNGISLLIFAGIISQLPASMSSIFGSVFTVNDPS
                     QQLHLLGLTLPLNAMAFWVSLGVGVASLIVLYILVKINEAQRVITINYAKRIKGNSTY
                     SGVKSILPVKLISAGVIPVIFAVAFLALPAFIGQVMVASNNPKFTGIGQNLVD"
BASE COUNT         1183 a         1089 c         1121 g         1007 t
ORIGIN      
        1 ttaaagagac cgccaaagcc aagactgcta agagcaccaa gaagggaggc aagaaataat
       61 ggcaacagct aaaactacca caaccgagcc tcgcttgaag actctttaca aaactacgtt
      121 caccaaagaa ctacaagacg agctagagct caagaatgtc aatcaagtgc cgaaacttga
      181 gaagatcgtt gtcagtgttg gcatcggtaa acacaaagac gataagcgct accatgagct
      241 ggtcgttaac aacctgcgta agatcactgg ccaacagcca gttgatcgca tggctcgcaa
      301 atcgattgct accttcaaga ttcgtgccgg tatgaaccga gtcggcgtca gtgtaaatct
      361 acgtggcgct cgtatgtacg aattcctcga ccgcctagtc aacgtggcta tgccacgaat
      421 ccgcgacttc catggcgtcg gcgccaaggg ctttgatcgg tcgggtaatt acaacctagg
      481 catcgtcgag caatcgatct tcccagagct cagctttgaa gacacccagg ttttgcatgg
      541 tatgcaaatc actttcgtag ttgagtctgg atctcgtgct ggcagtcgat tactactaga
      601 aaaatttggt gtaccgtttg agaaaggagg caagtaatgg ccaaaaaatc tatgatcgct
      661 cgtgacaaaa aacgacagaa aatgattgaa aaatacgccg ctaagcgagc tgagctgaaa
      721 gccgctggtg acctagaagg tctggccaag ctgccaacta actctagccc gactcgctgg
      781 aaaaaccgct gtatggagtc cggtcggcca cacgcttaca tgcgtaagtt cggtctaagc
      841 cgtatcgctt ttcgagagca cgcttccaaa ggtgagatcc caggtgtaac gaaaggaagc
      901 tggtaatgag catgcaatct actgacccaa tcgctgatat gctgacccgt atcaggaacg
      961 ctatcatggt tggccagacc gaagttcgtc tgcctagttc caagcttaag gttactgtag
     1021 ctaaagagct gaaaaaggct aactatctgc acgacgttaa ggttgaaaag ggtagcccgc
     1081 gtgacaccct ggtcatcacc atcaatgcag ctggccagaa tgcgctgatc aaccatgtgg
     1141 ctcgtgtatc gcgcccaggt cgccgtgttt atagctcggc tgccgacatc ccacgtgtta
     1201 aaaacggacg tggcttgatc ctagtcagca cctccaaagg tgtcatggac ggccaagcag
     1261 ctaagaaagc ccgccttggc ggtgagctga tctgcgaagt ttattagaag tttaaaggag
     1321 aaagtatgag tcgaatcgga aaactaccca tccaaattcc tcagggcgtg acaatcacgg
     1381 ttgactctga cgctgtacgc gttcagggcc ccaagggaca actcgagcag ttcttgctgc
     1441 ctgaagtcaa agtcaaggtc gaagacggcc aagctctggt tgagcgtgtc gatgacagca
     1501 agctcgctaa aagccgccac ggcttggtgc gatcgcttat caacaacatg gttacaggtg
     1561 tgaccgaagg cttttctaag accttggaga tcaagggcgt tggtttccgc ctagaaggtg
     1621 gcggccgcga aatccaaatg agccttggtt tctcgcaccc gatcaaatac caagcccctg
     1681 aaggcgttga tctgaaagtc gataagatga atctaacagt ttcaggcatt gacaagcaga
     1741 aagtcggcca agttgccgct gagatccggt ccttcaagaa gcctgagcca tacaagggca
     1801 agggtatccg ctacgagggc gagcaaatta tccgtaaggc aggaaaggca ggtgcgaaat
     1861 aatgagccaa ttagctaaga aattactcaa tcaatccttg cgcaaaggcc gtgtccgcgc
     1921 ccgcgttagt ggcagcgcaa cgaagccgcg cctaactgtg actatgtcta acttacatgt
     1981 cagtgcacag ctaattgatg atgacaccgg ccgaacacta gctagtgcca ctactgttgg
     2041 tactaagcag acaggtaaca tgaccgataa agccgcctac gtcggtagcg aaatagccaa
     2101 gaaagccaag caggctaaga tcacaaaagt agtatttgac cgtaatggcc gggcttacgc
     2161 caagagattg aaggcgttag ccgacgcggc ccgagagaaa ggtttggagt tttagtatgc
     2221 cagagaatac agccaataga caacgccagc gctcgccacg tgacactagg ccgcgtgaag
     2281 acaagctctt tgaagagcag gttatcaata tcgaccgtgt tgctcgagta gttaagggtg
     2341 gtcgtcgctt ccgtttcaaa gctctagttg ttgtcggtga caggaagtcc aaagtcggcg
     2401 tcggtgtcgc taagggccaa gacgttcaag cggctatatc aaaagccact gacgtagcca
     2461 agaagagcat gatcaccgta ccggttaagg acggcactat tccgcacgac atcgaagtta
     2521 aattcggtgg cgcacgggtg ctcttgaaac cagctgctcc aggtaccggt attatcgctg
     2581 gtggtgctat ccgaactatt atcggtgtta ccggcatcaa caatctgttg tcgaagcgcc
     2641 taggctcaac caacaaagcc aacatcgcct acgctaccat caaggcattg gacgagctgg
     2701 ttccagctga agagtggttg aatcaaccta agcaatctaa aaaagccgag gttaagaagt
     2761 aatgaagtat aacgaactaa catcgccatc acgtcaaggt cgcaagcgcg tcggtcgcgg
     2821 tatcgctgcc ggcggcggta agacggctgg tcgcggtacc aaaggtcaaa atgctcgtac
     2881 cggcaagaag ctgaacgcta ccttcatggg tggtcagcga gctctagtcc aggctatacc
     2941 gaagaagaag ggttttcgtt ccctaagggc gccagctcaa gtcgtttatc tagatcaact
     3001 caatgacctg aaagccacta ccatcgataa ctttgctttg gccgaagctg gcttgatcag
     3061 cacaccacac cacagtgtca agctgattgc caggggtgac tacaacggca aagctaccgt
     3121 caaggttcag gctgctagcg ccagcgtcaa gcaagctatc accaagagtg gtggtaagtt
     3181 tgaggccagc ccagtaccgc tcaagaaggc cgacaagacc actaaataat cggactgagt
     3241 ctagcttaaa atcctcgtcg aactgacggg gattttaatt tatagtaccg ttcggttgca
     3301 ctagaggccg gtttggcttt aattccgatg ttactcgagc cataaattcg tgtataatta
     3361 actgcgttga cgctgtgcca taatgcacag ctaggaaaga tacaattact ctatgaattg
     3421 gaaaactatt ttcgcttcac tgaagagcgc tgacatgagg aagcgcctct tgaccgtgct
     3481 cggcatcgtc gtagcctacc ggttcattgc tcagattccg gtaccgatag ccgaaccgac
     3541 ccaacttcgt caaatcttac agaacacctt cagctcgagt gacctgggtg gtttcttgaa
     3601 cctattgtcc ggtggagcat tgactagtct atcggtcgtc ttggtcggac ttagccctta
     3661 cattacggct agtattatca ctcagctact gactaaagcc atccctaagc ttgaggaatt
     3721 gcaacgcgat ggcggtgagg ctggccgacg taagatcaat caatggacgc gtattatcac
     3781 gctaccacta gccattgttc agtcgattgc tttcatctac ttgatccgtc agacggtgtt
     3841 agccagcaat accgccttgc ttggtcagac tacgctatac gaatgggttc tcgctgttac
     3901 ggctatgaca gccggcgcca tggtgatgat gtggctaggt gagctcatca ccgaacaagg
     3961 agttggtaat ggtatttcgt tactgatctt tgccggtatt atatctcagc taccagcctc
     4021 gatgagtagc atatttggtt cagtcttcac agttaacgat cctagccagc aacttcatct
     4081 actaggcttg acactgccgc ttaacgctat ggccttctgg gtatcgttgg gcgtgggagt
     4141 agcttctctg atagtcttat acatcttagt taagatcaat gaggcccagc gggtcatcac
     4201 tatcaattac gccaaacgga tcaagggcaa tagcacttac tcgggtgtta agagtatctt
     4261 accggtcaaa ctgatatcag ccggcgtgat cccggtcatc ttcgccgtgg ctttcctggc
     4321 tttgccggca tttatcggtc aagttatggt agcctctaat aaccccaaat ttaccggaat
     4381 cggccagaac ttggtcgatt
//
LOCUS       DASSBW010000078         3368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1039540, whole genome shotgun sequence.
ACCESSION   DASSBW010000078 DASSBW010000000
VERSION     DASSBW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039540"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFG56_02245"
     CDS             <1..366
                     /locus_tag="VFG56_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6622733.1"
                     /translation="GCLDQHEVTPTDWNFLGAVLNAPDGIRLVKLADHLGVKAPLVTA
                     IANRLMSRGLIKRIPHHTDRRAKLLVITPEGKRFVDSVEHDMENALQRLTLGLSVDDL
                     TAYQRVLDTIIANAKRTND"
     gene            470..1144
                     /locus_tag="VFG56_02250"
     CDS             470..1144
                     /locus_tag="VFG56_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622734.1"
                     /translation="MGNSGKTAAIAIVTLVVGLVVGFGAAKAMDDEMNGGHDMASMTK
                     TSDKASDLRANLVTLGVEHMTYTDQAVAAALDGAPNAEQLAELLYDNGDQIGAAVGSF
                     YGDEAEKTFDQVWKLHLDQFVNYAVASSKGDEAAKQAALDKIDSGYTKPLAQFLAKAN
                     PNFDEQTLETALRDHVNMTAKMIDFHVDGNYQKEADELNMANKHIEGIMSVLAAGIVK
                     QFPDKF"
     gene            1242..1688
                     /locus_tag="VFG56_02255"
     CDS             1242..1688
                     /locus_tag="VFG56_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET6622735.1"
                     /translation="MNRKLLGVTITLVIVTIIAVVAAVAVTNQSSDEGAEHEHGSHSH
                     QEGEQATGEDSTADKEAVVTDRVTIEQYAFSPATITIKAGTTVTWTNRDSVNHTVTSD
                     DGSAIKFDSGDMAQGQTYQFTFDQPGTYNYHCTPHPFMRGTVVVTE"
     gene            1690..3042
                     /locus_tag="VFG56_02260"
     CDS             1690..3042
                     /locus_tag="VFG56_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HET6622736.1"
                     /translation="MSRQHLLDERPSGWQVGLIGAWLVNLAVIGIIWSTSSDFRGLAG
                     WLDAAGMLFGLLAVYFALTQFMLMGRLPWLEGAFGLDRLAGYHRLNGYMAISFILIHP
                     LFITWSYAVAAQVSPIRQYVDLIVNYPYVWLALIGEILFLAVVASSIYMVRRRLKFEN
                     WYFVHLAVYLAIIFSAIHPFFVSGSFAGTAHPLAKVYWLSLYGFVGLNLLAWRLVWPL
                     LTSWQLAFRVERVVKETPSTTSVYITGRGLDRLAARPGQFVMVRVLQSGFWLQEHPFS
                     LSLIQPNQLRLTIRRIGDYTSKVGQINPGSRLLISGPFGRFTEAVADTDKRLFIAGGV
                     GITPLRPLAEQALAEGRHSILIYGNQHVDDVPLKNEMDQLAERGLELNYVFSDEPPEP
                     SRLSGYIDLKLIKKLAPDYLDRDIYLCGPPAMMDVLIEQLQADGLAAGRLHFERFRLP
                     "
BASE COUNT          846 a          788 c          852 g          882 t
ORIGIN      
        1 ggctgtcttg atcaacatga ggtcactcca actgactgga acttcctggg agctgtttta
       61 aatgctccgg acggtattcg gttagtcaaa ctagctgatc acctcggtgt taaagcgccc
      121 ttagtcacgg ctatcgccaa ccgcttgatg agtcgaggct tgatcaaacg tataccgcac
      181 catactgatc ggcgagccaa gttattggtt ataacacccg aaggtaagcg cttcgtcgac
      241 agtgtagagc acgatatgga aaatgccttg cagcgactaa cgcttgggtt atcggtagac
      301 gatctgaccg cttatcaacg ggttcttgac actattattg ctaacgccaa gcgaactaac
      361 gactaacttg tttaagcaaa cggcactact tgtcgacgct ttgttgactt aaaaaaagtt
      421 aaggtgttta attaaataca ttaactattt aataaaggag gtttagttaa tgggtaattc
      481 tggtaaaaca gccgctatag caatagtcac cctggtagtc ggtcttgtgg tcggcttcgg
      541 tgccgccaag gcgatggatg atgaaatgaa tggcggtcac gacatggcaa gtatgacaaa
      601 aactagcgat aaggcgtctg atttgcgcgc caatcttgtc acgctcggtg tagagcacat
      661 gacttacacc gaccaggcgg ttgcggcagc actagacggc gcgccaaacg ctgagcaact
      721 agccgagctt ctttatgaca acggtgacca aatcggtgca gcggtcggca gtttctatgg
      781 ggatgaagct gaaaagactt tcgatcaagt ttggaagcta caccttgacc aattcgtcaa
      841 ttacgccgta gcttcgagca agggcgacga ggctgccaag caagccgcgc tagataagat
      901 tgacagtggc tataccaagc ccctggctca gttcttggcc aaggctaacc ccaactttga
      961 cgagcaaacg ttagagacag ctctgcgtga tcacgtcaat atgacggcaa agatgatcga
     1021 ttttcatgtc gatggaaact accaaaaaga agccgacgaa ctcaacatgg ccaataagca
     1081 tatcgaaggc atcatgagtg ttttggcggc tggtatagtc aaacaattcc cagataagtt
     1141 ttaaacaagc tatactaact atagtattag tttaagccgc tttaaaaggg taggtccaat
     1201 cagatgagcg acctaccctt tggcgtctaa aggaggttta tatgaacagg aagctactag
     1261 gtgtaactat aaccttagtc atagtcacca tcattgcggt ggtagctgca gttgcggtaa
     1321 ccaaccaatc atcagatgag ggtgccgagc atgaacatgg cagccatagt caccaggagg
     1381 gtgagcaagc caccggcgag gactcgactg ccgacaagga ggctgtggtt actgataggg
     1441 taacgattga gcagtatgct ttttcaccgg ccaccattac gattaaagcc ggtactacgg
     1501 tgacttggac taaccgcgat agtgtcaacc acaccgttac ctctgacgac ggttcggcta
     1561 taaagtttga tagtggcgat atggcccaag ggcaaaccta tcagttcact tttgatcagc
     1621 caggaaccta caactatcac tgtacgccgc acccgttcat gcgtggcact gttgtagtaa
     1681 ccgagtaata tgtctcggca gcacctcctc gacgaacgtc cgtctggctg gcaagtgggt
     1741 ctgatcggtg cttggctggt caacctagcg gttattggca tcatctggtc aactagttct
     1801 gatttccgcg gcttagctgg ctggctggat gccgctggta tgttgttcgg cctacttgca
     1861 gtttactttg cgctgacaca gttcatgctg atgggccgtc tgccatggct agaaggggct
     1921 tttgggctgg atcgtttggc cggctatcat cgcctgaacg gttacatggc gattagtttc
     1981 atattgattc acccactatt tattacctgg agctatgcag tggcggctca agtctctccc
     2041 atacgccagt atgtcgattt aatcgttaac tatccctatg tctggctggc cctgatcggt
     2101 gagatattgt tcttggcggt ggtagcaagt tctatttata tggtccgacg tcggctaaaa
     2161 ttcgaaaact ggtacttcgt ccatctagcg gtttatctag caattatatt ttcggctatc
     2221 catccttttt ttgtaagcgg cagcttcgcc ggcacggccc acccattagc caaggtctac
     2281 tggttgagcc tttatggctt tgtcggtttg aatctcttag cttggcggct agtttggcca
     2341 cttttgacca gttggcagct agcttttcgc gtcgagcgag tcgttaaaga aacgcccagc
     2401 actacctcgg tctatataac agggcgtggt ttagacaggt tggctgctcg tccaggtcaa
     2461 tttgtcatgg tccgagtact tcagtctggc ttctggcttc aggaacaccc cttctcactg
     2521 tctctcatac agcccaatca gttgcgattg acgattcgtc ggatcggcga ctacacctct
     2581 aaggtcggcc agatcaatcc cggcagtcgc ttattgatca gtggaccgtt cggccgattc
     2641 accgaggcgg tcgccgacac cgataaacgg ctattcatcg ctggcggggt agggatcaca
     2701 ccgctcaggc cactggcaga acaagcctta gcggaaggcc ggcattcgat tctgatttat
     2761 ggaaatcagc atgtcgacga cgtgccgctt aaaaacgaga tggatcagtt agccgaacgt
     2821 gggcttgagt taaattatgt atttagtgac gagccacccg agccgagccg attatctggc
     2881 tacatagacc tgaagctaat caaaaaattg gcgccggatt acctcgaccg cgatatatac
     2941 ctgtgtggcc cgccagccat gatggacgta ttgatcgagc agctgcaggc agatggttta
     3001 gcggccgggc gtctacattt tgagcgcttc agactgcctt aaagggtcta aaataatctg
     3061 gacgaaaaaa cagccgcctg cgaggaacgg ctgttttgaa ttttgtttgg tctttttgat
     3121 ttttggctcg tgagtaagtt tattattcaa gagctacccc tataataagc cgtgaaaaag
     3181 ctaatgtcaa tgacttttta aaagattttt tatggacatt tttacctaca aaaagtagga
     3241 ttatcgatca cccgagcagt attaatggca tgaagcaatc tatcttagaa taatccgact
     3301 cagctcgggt ccatacctga cattcagccg gcagaatttg ggacgatttg tgtataaagg
     3361 gtgagtca
//
LOCUS       DASSBW010000079         4416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_331608, whole genome shotgun sequence.
ACCESSION   DASSBW010000079 DASSBW010000000
VERSION     DASSBW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331608"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFG56_02265"
     CDS             complement(<1..586)
                     /locus_tag="VFG56_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6622737.1"
                     /translation="MANKTESKPAKPPVNWVARIAGYVLVAVLAFVAGNNTTDLVALI
                     KGKSGPTNLDLSSVQDLYDQLRVNFDGQIDAQKLLDGAKHGLVSATGDPHTVYFTAEE
                     ARQFMDDVNNTFSGIGAEIGKQGDKLIVVSPLDGSPAKQAGLKSGDIIAKVNGESTDG
                     WSIDKAVSKILGPALTTVKISVIRGVNLKEISITR"
     gene            643..1671
                     /locus_tag="VFG56_02270"
     CDS             643..1671
                     /locus_tag="VFG56_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HET6622738.1"
                     /translation="MSDLDKLRFESVDDKSYNKIIQSHPSRSWPIEQSPAWGDFQSRL
                     GGRRHIGNFVLYSGEEVVATASLLAMSMRGYSYVWINNGPIILNDSVKTDVLIEAVKT
                     LVRRHTTDLKPLFIRLSLPVEQSPTKALKPAYSHSLLEKTTAIDLNLSEEGILAGMKQ
                     GARRSIRKAEKAGVSIKHFSGDVALEQFSSLYDIMEETAGRDGFSAHPAKAYEAMLKE
                     LGDLARLYVGYNSDNHPVTWAIVTQYQHKAIYYYGASNAEARAIMAPYLMHWQIMKDM
                     KASGCKSYDFLGIGSDKYPGLEGVTQFKLKFGGPVLDYPSVYDLPLNSAKYKLWRTAQ
                     AARHKLKH"
     gene            complement(1678..2793)
                     /locus_tag="VFG56_02275"
     CDS             complement(1678..2793)
                     /locus_tag="VFG56_02275"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET6622739.1"
                     /translation="MKQRSTTPAFKRFATVAGLTVAVLAVVLSVPLQTYADIYDRKIK
                     ALQNQINGYQSEASDLSAKADTLKNAVAKFNNQIAAIETKISLSEAKRAELVKQIDAN
                     KKKLDLNKQAFADTLGDMYVDGTPSPIEILASSSSITDYVDKQSYRDAMNKRLEQTIK
                     EIDALQKKLAEQKKDVENTLRDQKSQQSQLAAKRSERNQLLAETQGKEASYQRLIKSS
                     RAKIADLRAQQIAANARFFGAAGHGPACGGGYPGKWCNIPKDYVVDDWGMFNRECVSY
                     TAFRVAASGRHMPYWGGSGNANQWDDNARASGIPVSGTPRAGDVAISNAGYYGHAMYV
                     ESVNGDGTINISQYNVDFTGTYSTARIGTGGLVFIHF"
     gene            complement(2877..3872)
                     /locus_tag="VFG56_02280"
     CDS             complement(2877..3872)
                     /locus_tag="VFG56_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET6622740.1"
                     /translation="MIKQKAKDVRVKLAQNKKLHRRFLTWLRMARYGANNFTRNAWLS
                     TAATAVMTITLIIIFTTVAATSILSSTVNDIKQKVDISIYMKNSVSQAQVDQMSDKLR
                     QDENVRSVDYIDLSEARDIYIRENDPSEAQLKLISQYSPEKSPFPPSLSVGLKDPTDF
                     SSIQHLVETDEDFKSAVNPDRPPSYTGKNRKIIDTIGGWVNNAQKGGLALSIVFVAIS
                     MLIIFNTIRMAIFNRKDEIQMMKLIGADRKFIRGPFVVEAVMYGFVAALAATVLGFLG
                     LVQLQPVLAERGVAIDDTKNLFTTWWPLILLAMIIIGAIIGMISSRLAVRRYLRV"
     gene            complement(4036..>4416)
                     /locus_tag="VFG56_02285"
     CDS             complement(4036..>4416)
                     /locus_tag="VFG56_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6622741.1"
                     /translation="AFALEIAGMTNREINATVPKVIELVGLTGKEKKFPHQLSGGERQ
                     RVAIARAVVRQPKILIADEPTGNLDPKHGWDIVRLLEKINRYGTTVLLTTHNAEIVNK
                     LKRRVITIDHGKITSDQAQGSYKQ"
BASE COUNT         1054 a         1103 c         1086 g         1173 t
ORIGIN      
        1 ggcgtgtgat tgagatttct tttaggttga cgccgcgtat gacgctaatc ttaaccgtcg
       61 taagggccgg acctagaatt ttagataccg ctttatcgat cgaccagccg tcggtcgatt
      121 caccgttaac cttggcgatg atgtcacccg acttcaaacc ggcttgtttg gccgggctac
      181 cgtcgagcgg gcttaccact atcagtttgt cgccctgttt gccaatctcc gcacctatgc
      241 cggaaaaggt attgttgacg tcatccatga actgcctagc ttcttcggca gtaaagtaaa
      301 cggtatgagg atcgccggtg gccgaaacca agccgtgctt ggcaccgtcc aataactttt
      361 gcgcgtcaat ctgcccgtcg aagttaactc gtaactgatc gtacaagtcc tggactgaac
      421 ttaagtctag attagttggt ccggactttc cctttatcaa tgccaccagg tctgtggtat
      481 tgtttccggc cacaaaagct agcaccgcca ccaggacgta gccggcgata cgagccaccc
      541 aattgaccgg tggtttagct ggtttggact ccgttttatt tgccataaaa gcttaccctt
      601 atactagcac gtaccccggc tctttggtaa tataaaaata tcttgagtga cttagataaa
      661 ctgcggttcg aatcagttga cgacaaatcg tacaataaaa ttattcaatc tcaccccagc
      721 aggagctggc cgattgaaca aagtccagcc tggggcgact ttcaaagccg gttaggcggc
      781 cgccgacaca tcggcaactt cgtcctttac tccggcgaag aggtcgtggc aacggcatca
      841 ctactggcta tgtcgatgcg gggttatagc tatgtctgga tcaacaatgg accgatcatc
      901 ttaaacgatt ctgtgaagac cgatgtcctg atcgaagccg taaagaccct ggtacgacgc
      961 cacacgaccg atcttaaacc actgtttatc cgcctgagcc tgcccgtgga gcaatcaccc
     1021 actaaggcac tgaagccggc ctattcacat agcttgcttg aaaagaccac tgccatcgat
     1081 ttgaacttat cggaagaagg tatcttggcc ggcatgaaac aaggtgctag gcgttctatc
     1141 cgtaaagccg aaaaggctgg ggttagtatt aagcacttta gtggtgatgt agcacttgag
     1201 cagttctctt ctctttacga catcatggaa gagacagccg gacgcgacgg tttctcggct
     1261 catccagcca aagcctacga ggctatgctt aaagaactgg gcgatctagc tcgcctttat
     1321 gttggctata actccgacaa ccacccggta acctgggcta ttgtcaccca gtatcagcac
     1381 aaagccatct actattatgg tgcttccaac gctgaagcca gggccatcat ggcaccctac
     1441 cttatgcatt ggcagatcat gaaagacatg aaagcctctg gatgtaagtc ttatgacttc
     1501 ctcggtatcg gctcggataa ataccccggt ctagagggcg taacacagtt caagctcaaa
     1561 tttggcggtc cagttttgga ttatccatcg gtctatgatt taccgctaaa ctcggccaaa
     1621 tacaaactgt ggcggacagc tcaagctgcc cgccacaagt taaagcacta gttttcttta
     1681 gaagtggatg aacactaggc cgccggtacc gatccgggct gtactgtagg taccggtaaa
     1741 gtcgacattg tactggctaa tattgatcgt accgtcaccg ttaaccgatt caacgtacat
     1801 ggcatgaccg tagtaaccgg cattagagat ggcgacatca ccagcccgtg gcgtaccgct
     1861 taccggtatg cccgaagccc tagcgttgtc gtcccattgg ttggcgtttc cgctaccgcc
     1921 ccagtaaggc atgtgtcgtc cagaggcagc cacacgaaaa gcggtatagc tgacgcactc
     1981 acggttgaac ataccccagt catcgacgac atagtctttg gggatattgc accacttgcc
     2041 agggtagcca ccaccacaag ctgggccgtg accggctgca ccgaagaagc gggcgttggc
     2101 tgcgatctgt tgagccctca ggtcagcgat ttttgcccgg ctactcttga ttaagcgctg
     2161 ataggaagct tctttacctt gtgtctcggc caatagttgg ttgcgctcag atcgcttagc
     2221 agccagctgg gattgttgtg acttctggtc tcgcaaggtg ttttcaacat cttttttctg
     2281 ctcggccagc ttcttttgta gtgcgtcgat ttctttgatg gtctgctcta ggcgcttatt
     2341 catagcgtcc ctataagact gtttgtcgac atagtcggtg atagagctcg acgatgccag
     2401 aatctcaatc ggactcggcg tgccatcgac atacatatca cctagcgtat cggcaaaggc
     2461 ctgtttgttt agatcaagct ttttcttatt ggcatcaatc tgtttgacta gctcggctcg
     2521 cttggcctca gataagctaa tcttggtttc gatagcggca atctgattgt tgaacttggc
     2581 tacggcgttc tttagagtat cagccttagc cgatagatca gaggcctcgg attgatagcc
     2641 gttaatttga ttttgtaagg ctttgatttt tcgatcatag atgtcggcgt aagtctgtaa
     2701 tggcaccgac aacaccactg caagaacggc tacggtcaga cctgcgaccg tcgcaaaacg
     2761 tttgaaagct ggtgtggtgg acctttgttt cataatgctt ctcctttaaa ataacacaac
     2821 tttagcttaa gcgcaatgat ttttacgcct attctgggtt caaaatatag agcaatttac
     2881 acgcgcaaat accgtcgaac tgccaatcgc gaactgatca taccgatgat ggctccgatt
     2941 attatcatgg caagcaggat gagcggccac caggtggtaa acaagttttt ggtgtcatcg
     3001 atggcgacgc ccctttcggc caaaactggt tgcagctgaa ctaatccaag gaaaccgagt
     3061 accgtagcag ccagtgccgc cacgaagcca tacataacag cttcgacgac aaacggacca
     3121 cgtatgaact tgcggtcggc accgattaat ttcatcatct gaatctcatc tttgcggttg
     3181 aagatagcca tgcgaatggt gttgaaaata atcagcatag agatggcgac gaagacgatc
     3241 gaaagcgcta aaccaccttt ctgggcgttg ttgacccaac cgccgatagt gtcgataatc
     3301 ttacggttct tgccggtgta gcttgggggt cggtcgggat taacagccga cttaaagtcc
     3361 tcatcagtct ctactagatg ctgaattgaa ctaaagtctg tcggatcctt caaccccacc
     3421 gataaacttg gcggaaacgg cgacttctct ggcgaatatt gagaaatcag tttaagctga
     3481 gcttcgctag gatcgttctc tcggatatag atatccctgg cttcgctcag gtcgatgtaa
     3541 tcaacgctgc gtacgttctc gtcttggcgt aacttatcag acatttgatc aacttgtgcc
     3601 tggctgacac tgtttttcat ataaattgag atgtcgactt tctgcttgat gtcgttgacc
     3661 gtggaactca ggatactggt agctgctaca gtcgtaaaaa tgatgatgag tgtgatcgtc
     3721 atcaccgcgg tagccgcagt tgacagccaa gcgttacgag tgaagttgtt ggcgccgtag
     3781 cgagccatcc tgagccaagt caagaagcgc cgatgcaact ttttattctg cgccagtttg
     3841 acgcgaacgt cttttgcctt ttgctttatc atctggctta ctttaatatc cttcttgtta
     3901 gcgaaaatgg caggttacgg tgcgaaatac gtgaagcgtg acgagacata gccaaagcta
     3961 cgtcgaggag cgctgagcgt gttgcagcct gtaaactgac gcttgtaggt agatataggg
     4021 tattcacgta agccattatt gtttataact tccttgtgct tgatcagatg taatcttacc
     4081 gtggtcgatg gtaatcaccc ggcgttttag cttgttgacg atctcggcgt tatgggtagt
     4141 caaaagcacc gtggtaccgt aacggttaat cttctcgagt aaccgcacga tatcccagcc
     4201 atgcttgggg tccaggttac cggtcggctc atcggctatt aggattttcg gttggcggac
     4261 taccgcccga gcgatagcta ctcgctgacg ctcaccgccg gacaattgat gtggaaattt
     4321 cttttccttg ccggtcagac cgaccaattc aatgactttc ggtaccgtcg cgttgatctc
     4381 acggttagtc atgccggcga tttccagggc gaaagc
//
LOCUS       DASSBW010000080         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_562242, whole genome shotgun sequence.
ACCESSION   DASSBW010000080 DASSBW010000000
VERSION     DASSBW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562242"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1565)
                     /gene="atpD"
                     /locus_tag="VFG56_02290"
     CDS             complement(150..1565)
                     /gene="atpD"
                     /locus_tag="VFG56_02290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6622742.1"
                     /translation="MPEATKTKTIEGKITQIVGVVVDVEFAAKDLQELNTALSVSHND
                     QEIVLEVALQLSETVVRTIAMSSTDGLRRGQAVTSTGAPISVPVGEDTLGRMFNVIGQ
                     PIDGQKGSFKKKSSIHRSAPPLTEQSGEVEILETGIKVIDLIAPVTKGGKVGLFGGAG
                     VGKTVLIQELINNIAKFHSGYSVFAGVGERTREGNDLYGEMSESGVIKNTALVFGQMN
                     EPPGARMRVALSGLTIAESFRDQGKDTLLFIDNIFRFTQAGSEVSALLGRLPSAVGYQ
                     PTLQQEMGGLQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDSTITLSRALT
                     EIGIYPAVDPLDSTSTILDPEIVGEDHYNVAREVQRVLQRYKDLQDIIAMLGMEELSE
                     EDKQTVSRARKIQRFLSQPFFVAEQFTGTPGQYVKLEDTIAGFKEILDGQLDDYDEQD
                     FYLKGSIEQVKKDKAAKSKKA"
     gene            complement(1579..>2058)
                     /locus_tag="VFG56_02295"
     CDS             complement(1579..>2058)
                     /locus_tag="VFG56_02295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HET6622743.1"
                     /translation="GDEPTENDIRPILDFIVDQYAEARIDSVRLIYTVFKSSIVQEAT
                     NLALLPAKLEPRKSDQVKPAGDIDKIDEVDMNFEPDVETVVHHVTARLIESQIWQALL
                     ESLASEYAMRMMAMKNATDNANDLIDDYTLEYNTARQAAITQELAEITGGAEALSET"
BASE COUNT          475 a          534 c          519 g          530 t
ORIGIN      
        1 ttagccgccg aaaccagcgg catatggccg ggcaagacgc cgatttggcc atcgattgtc
       61 ggcaggacta cctcgtagac gtcaccttca aatttggtac cggtcagtgt gactaatttt
      121 agatgtagac tgctcaattg aagctccctt taggctttct tagatttagc ggctttatct
      181 tttttgactt gctcgatcga gcccttcaga tagaagtctt gttcatcgta atcatccaat
      241 tggccatcta aaatctcttt gaagccagca atagtgtcct ccagcttgac atactgaccg
      301 ggagtacctg taaactgctc ggctacgaag aagggctgag acaagaaacg ctggatctta
      361 cgtgctcggg agacggtctg tttatcttcc tctgataatt cttccatgcc gagcatggcg
      421 atgatatctt gcaagtcctt gtagcgttgg aggactcgct gaacttcacg cgctacattg
      481 tagtgatctt caccaacgat ttccggatca aggatggtcg aagtcgaatc aagcgggtca
      541 acggctggat agataccaat ctcggtcaaa gcccgtgaca gggtaatcgt cgagtcaagg
      601 tgagagaagg tggtagctgg agccgggtcg gtcaagtcgt cggccggcac gtagaccgcc
      661 tgaaccgagg tgatcgaacc cttcttagtc gaagtaatcc gctcctggag accgcccatt
      721 tcttgctgaa gagtcggctg gtaaccgacg gccgaaggta accgaccaag cagggcggaa
      781 acctcagagc cagcttgagt aaagcggaag atgttatcga taaataacag cgtgtctttg
      841 ccttgatctc ggaagctctc agctatagtc agacccgata gggcgacgcg catacgagct
      901 cctggtggct cgttcatttg tccaaacact agagcggtat ttttgataac accggattca
      961 ctcatctcac cgtatagatc gttaccttcg cgggtccgct caccgacgcc ggcaaagacg
     1021 gagtagccgg agtgaaactt agcgatattg ttgatcagtt cctggataag cacagtctta
     1081 ccaacaccgg caccgccaaa caggccgacc ttgccgccct tggtgaccgg cgcaatcaaa
     1141 tcgataactt tgataccggt ttccagtatt tcaacttcgc cagattgctc ggttaacggt
     1201 ggagccgacc ggtggataga cgacttcttc ttgaaagagc ctttttggcc gtcgatgggt
     1261 tgacctatga cattgaacat ccggcccaga gtatcttcgc caaccggtac tgatatcggc
     1321 gcaccggtcg aagtaacggc ctgcccacgc ctcaaaccat cggtcgagct catagctatg
     1381 gttcggacta cggtctcaga cagctgcaag gcgacttcca gtactatttc ctggtcgtta
     1441 tgacttacgc tcagggcagt gtttagttcc tgtaaatcct tggccgcaaa ctcgacatcg
     1501 acgaccacgc cgacgatttg agtaatctta ccttcaattg ttttagtctt ggttgcttca
     1561 ggcataaaat ctccttatct aagtttcact taaagcttcg gcgccaccgg taatttcagc
     1621 caattcctgg gtaatggcag cttggcgggc agtgttgtat tccaaggtgt aatcatcgat
     1681 caagtcattg gcgttgtcgg ttgcattctt catagccatc atccgcatgg catactcaga
     1741 cgccaggctc tcaagtagtg cttgccaaat ctgcgactca atcaatcgcg cggtgacgtg
     1801 gtgaactacc gtctcgacgt ccggctcgaa gttcatatca acttcgtcaa tcttatcgat
     1861 gtcgccggct ggcttaactt gatcgctctt acgcggctct agcttggctg gcaacagggc
     1921 taagttggtc gcttcttgca cgatcgacga tttaaacacc gtatatataa gccgtacgga
     1981 atcgatccgc gcctcagcgt attgatcgac gatgaagtcc aggattggtc ggatgtcgtt
     2041 ctcggtcggt tcatcgcc
//
LOCUS       DASSBW010000081         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1411684, whole genome shotgun sequence.
ACCESSION   DASSBW010000081 DASSBW010000000
VERSION     DASSBW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411684"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFG56_02300"
     CDS             <1..602
                     /locus_tag="VFG56_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6622744.1"
                     /translation="LQKAHENAQHLGRLFQDLLDVSKVEDGRMKNNPTIIDAVSFSKK
                     VYDNFTESARAKGLELKFMSKSIGTVSPLYYVKADADKLEEVLSNLVENAIKYTPQGS
                     VSVDVSGDNELVKFSVSDTGLGIPAEDIPHLFQKFYRVDSSDTREIGGTGLGLYLSRR
                     LIESMDGHLRLDSQYGKGSVFTIELRRAQGSDMPEISGL"
     gene            653..1642
                     /locus_tag="VFG56_02305"
     CDS             653..1642
                     /locus_tag="VFG56_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6622745.1"
                     /translation="MTKIMLVEDDKSLREIYGVRLLAEGYDIVSAGDGEEALAVAIKE
                     RPELVVSDVMMPKISGFDMLDILRSTTETKNIKVIMMTALSSEEQRQRGESLGADKYL
                     VKSQVGIEDVVRTVHEVLGDKPTAAPAAPAAPVPPANPAPAPQAAPQPAPAPAPAPPP
                     VSPAPAPQPAPAQPQPVQPVQPTPPPPASPPTAPPSPAAAPIPQTPQPNTDTPKPASL
                     KNRIIQPINDPSAEPVNMGELLDQELAKEAGLSEDDRPQVASTEQEKQSVEQQLDQIM
                     TDPNNNSDSDDQTNSDGSIVAGPNVEPPFQNDDQSNSSTTPPPPPPPPGAPTA"
     gene            1662..2354
                     /locus_tag="VFG56_02310"
     CDS             1662..2354
                     /locus_tag="VFG56_02310"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6622746.1"
                     /translation="MIDNDNFRLNKFLAHKLGLSRREADDMIAAGRITLDGEVAQLGA
                     RLRGGQSVKLDDKAIDLDKPAYTYLAFNKPVGYVCSRRSQGETPTIYKLLPDKYRNLK
                     TVGRLDRDSSGLLLLTDDGDFTFSMTHPKFHKTKTYEIELDKSLEPLHRQMISDHGIQ
                     LEDGLSRLHLDRLKEGDDNGWRVLMHEGRNRQIRRTFAAVGYQVVKLHRVQFGHYSLA
                     GLQSGEIDTITP"
     gene            2378..3142
                     /locus_tag="VFG56_02315"
     CDS             2378..3142
                     /locus_tag="VFG56_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622747.1"
                     /translation="MSESKEGQGGEIRLADIIQLEPHSGRMQELENCLRLAVEQWDDG
                     CIGFNNKPGTIYCYEEAADAVITQVRQAYADKGLLRHYDFSTISNHNQPITGQGVAHG
                     LKVMARYIYHCADHVSQVAPDTGLEELSQLLQSAVSRDSLRQLAREARYTNQHLERNL
                     GLLHTSSHQLETKVFEIGRRGMIACDYRQKRADVMDQLRAEGQPAEYIYSQPESFCKA
                     LPGGHWNRVYDAMVQLCVKDPRLMSSTMKRHRPSSN"
     gene            3320..4678
                     /gene="der"
                     /locus_tag="VFG56_02320"
     CDS             3320..4678
                     /gene="der"
                     /locus_tag="VFG56_02320"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET6622748.1"
                     /translation="MSQKLPYVAIVGQTNVGKSSLFNRLYGTQEAVVAREAGTTRDNV
                     VRQLDLHGKSFWLVDTAGLKPAEDEFEASIQEQIADATELADLIIVMVDSTKYPSNED
                     RRIAKTALKSKKPVLLVVNKTDLKGALASDEFKRLGIKDIFRTSAEHNQGVKRLAETI
                     AEQLPDIQSEMVSDKLKIALIGRPNVGKSNLFNRLAGKQQAIVANVAGTTRDVNRVDV
                     RYHGRDIELLDTAGVRRSGKQEVGIEKFSVLRTLAAIDEADICLLLMDVNELNTQLDQ
                     KLAGMIKDAGKGLVLVVSKWDAVEGKDAFTRDALAPQISYNFKFVPWASLIFTSAVTG
                     QNVTKIFDLALSIDERRNQQLKTRQLNDILQTCLRKHPPAGLKNTYPKLRYMVQTDTS
                     PPWFVIFGSNLKFLHWSYKRYLERTVRDTTDFAGTPIMFSFRDEKQIKANRDKQKESS
                     KD"
     gene            4703..5035
                     /locus_tag="VFG56_02325"
     CDS             4703..5035
                     /locus_tag="VFG56_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622749.1"
                     /translation="MTHRLLDTSPAHMTEALDDSVIPRIPIACPEPVLISETNSLITV
                     ATVTLVLSSLPEAVDKRGGGQKTEAYRNPSQKSRHPDVVTAERLVAAHQDNRPKDENH
                     QEKATILN"
BASE COUNT         1460 a         1402 c         1244 g         1148 t
ORIGIN      
        1 acctgcaaaa ggcccatgag aacgcccagc acctcggacg actattccaa gacctcctgg
       61 acgtctccaa ggtagaagat ggccgtatga agaataaccc gaccatcatc gatgccgtca
      121 gtttttctaa gaaggtctac gacaacttca ccgaatcggc cagggccaaa ggcttggagc
      181 tgaaatttat gtccaaaagc attggtacgg tcagtccgct ttattatgtc aaagccgatg
      241 ccgacaagtt ggaagaggta ctatcgaacc tagttgagaa cgccatcaag tatacgcctc
      301 aaggttcagt gtcggtcgac gtgtccggcg acaacgagct ggttaaattc agcgttagtg
      361 acacgggcct aggtatcccg gccgaagaca tcccgcacct cttccagaag ttctaccgcg
      421 tggacagcag tgacactcgt gaaatcggcg gcaccggatt gggcctttat ttatcgcgtc
      481 gactaatcga atccatggat ggacacctcc gactcgatag ccaatacggc aaaggtagtg
      541 tttttacgat tgagttaaga cgggcgcagg gcagcgacat gccggaaata tctggattat
      601 agcgtaagaa ctatggcatt ttattacaaa tctaattaca ataggactta atatgacaaa
      661 gattatgctt gtcgaagacg acaaatcctt acgtgaaatt tatggcgtta ggctgttagc
      721 cgaaggttac gacatcgtct cagccggtga tggcgaagaa gcactagctg tggccatcaa
      781 agagcgtcca gaactggttg tatcagatgt gatgatgccc aagattagtg gttttgacat
      841 gctcgacatc ctacgctcta caactgaaac taagaacatc aaagtcatca tgatgacggc
      901 gctcagctcc gaagagcaac gacagcgcgg tgaaagcctt ggtgccgaca aatatctggt
      961 caagtcacag gtcggtatcg aagatgtcgt acgaaccgta catgaggtgt taggcgacaa
     1021 gccaacagct gcaccggccg ctccggctgc gccagtacct ccagccaacc cggcgcctgc
     1081 gccacaggca gcacctcagc cagcaccagc tccggcaccc gcaccaccac cggtcagccc
     1141 tgcgccagca cctcagccag caccggcaca accgcaacca gtccagccgg ttcaacctac
     1201 gccgccacca ccagctagtc cgccgaccgc accaccttcg ccagctgcag cgcccatccc
     1261 tcagactcct cagccgaaca ctgatacgcc aaaaccagct tctctaaaga atcgtatcat
     1321 ccaaccgatc aatgatccgt ctgccgaacc cgtcaatatg ggcgaattac ttgaccaaga
     1381 acttgccaaa gaagccggcc ttagtgaaga cgacagacca caagtagcta gtactgaaca
     1441 agagaaacaa tcagtcgagc aacaactaga ccagattatg actgacccga ataacaacag
     1501 tgacagtgac gatcagacaa actctgacgg cagtatagta gctggaccaa atgttgagcc
     1561 gccgttccag aacgatgatc aatcaaactc ctcaaccacg ccgccacctc cgcctccgcc
     1621 accaggtgca ccgactgctt aaggcagtat aattcgtaag agtgatagac aacgataatt
     1681 ttcgcctgaa taagttcttg gcccataagc ttggtcttag tcgccgtgaa gccgacgaca
     1741 tgatagcggc tggccgcata acccttgatg gagaagttgc ccagttggga gcacgccttc
     1801 gtggtggcca atcggttaaa ttagacgata aagcgatcga tctcgataaa ccggcctata
     1861 cctacctagc ttttaacaaa ccggtaggct atgtttgttc acgccgaagc cagggcgaaa
     1921 ctccgactat ctacaagttg ctacccgata agtatcgcaa cctaaaaacg gttggtcgac
     1981 tagatcgcga tagttccggc ttactcctgc tgaccgatga tggtgatttt actttttcca
     2041 tgactcaccc taaattccac aagaccaaga cttacgaaat tgaacttgat aaatctttag
     2101 agcccttgca tcgacagatg attagcgacc atggcatcca actcgaagat ggacttagtc
     2161 ggcttcattt agaccgtcta aaagaaggtg acgataatgg ctggcgggta ctcatgcacg
     2221 aaggtcgcaa tcgccagatc cgccgaacct tcgccgccgt cggctatcaa gtcgtcaaat
     2281 tacatcgagt acagttcggt cattattctc tagcaggact tcagtcaggt gagattgaca
     2341 ctatcacgcc atagtggcgc agtttatact ctaagttatg tccgagagca aagaaggtca
     2401 gggcggagaa atccgactag ccgatattat ccagcttgag ccacatagcg gccgtatgca
     2461 ggaactagaa aactgtctac gcctggcagt cgagcagtgg gatgatggct gtatcggatt
     2521 caataacaag cctggcacta tctattgtta cgaagaggct gcagatgctg tcatcacgca
     2581 ggtgcgtcaa gcctatgccg acaaaggact gttacgtcac tatgatttta gtactatcag
     2641 taaccacaac cagccgatta ccggacaggg tgtggctcac ggcctaaaag tcatggcccg
     2701 ctatatctat cattgtgccg atcacgttag ccaggtggca ccggataccg gcctagagga
     2761 gttaagccaa ctactacagt cggccgttag tcgcgatagc ttgcgccaat tggcccgaga
     2821 agcccgctat accaatcaac atttagagcg aaacctgggc ttacttcaca cttcgagtca
     2881 tcaactggaa actaaagtct tcgagattgg tcgacgcggc atgatagcct gcgactaccg
     2941 tcagaagcga gcggacgtta tggatcaact cagagccgag gggcagccag ccgaatatat
     3001 ctactcgcaa cccgaaagct tttgcaaagc tctaccgggc ggccactgga accgtgtcta
     3061 tgacgccatg gtgcaattat gcgtcaaaga tccccgactg atgtcgtcta ccatgaaaag
     3121 acatcgtcca tcatcgaact aaactctgtt atgacatttt ttcaaattgt cataacttcg
     3181 ccttactcgg atatgaaagc gagctttcat atctcgcgtt tcgttgagtt ataatagagg
     3241 aacgcaggaa gataaaagcc tgcttatatt ttgcgattac cagatattat ctggttccgc
     3301 aggaagcaga taatattcga tgagtcaaaa actaccctat gtggccattg ttggccagac
     3361 caacgttggt aaatccagcc tgtttaaccg cctatacggt actcaggaag ctgtggtggc
     3421 acgtgaagcc ggtaccaccc gtgacaatgt cgtccggcag ctagatctgc acggcaaaag
     3481 cttctggctg gttgatacag cgggactcaa accagccgag gacgaattcg aagcttccat
     3541 tcaggaacag attgccgacg ccaccgagtt agccgatctt atcatcgtca tggtcgattc
     3601 aaccaagtat ccatccaatg aagaccgccg catcgccaag acggccctga agagcaaaaa
     3661 gccagttctg ctagtcgtaa ataagaccga tctgaagggc gctctggcga gtgacgagtt
     3721 caagcgcttg ggtataaaag atatcttccg aacttcagcc gaacacaatc aaggcgtcaa
     3781 acgccttgcc gagaccatag ccgagcaact accagacatc cagagtgaaa tggtaagcga
     3841 caagctaaaa attgccttga ttggacgacc aaatgtcggc aagagcaatc tatttaatcg
     3901 cctagccggt aaacagcagg caatcgtcgc caatgtcgcc ggcaccactc gtgatgtcaa
     3961 ccgtgtcgac gttcgctatc acggtcgcga catcgaacta ctagacaccg ccggtgtccg
     4021 tcgctccggc aaacaagagg ttggcataga gaaattctcg gttctacgca ccttagccgc
     4081 catcgacgaa gccgacatct gcttgctctt gatggatgta aacgaactca acactcaact
     4141 cgaccaaaag ctggccggca tgatcaaaga tgccggtaag ggcttggttc tagtagtctc
     4201 caaatgggat gctgtagaag gtaaggatgc cttcactcgt gatgccttag cgcctcagat
     4261 cagctataac tttaagttcg ttccgtgggc cagtctgatt ttcaccagtg ccgtcaccgg
     4321 ccagaatgtg accaagatat ttgacctggc attatccatc gatgaacggc gcaaccagca
     4381 gttaaaaacc cgccagctaa atgatatctt gcaaacttgc cttcgaaagc accctccggc
     4441 cggactaaaa aatacttatc ccaagctccg ctatatggtc cagaccgata cgagtccgcc
     4501 gtggtttgtc attttcggct ccaatctaaa gttcctgcac tggagctaca aacgctatct
     4561 ggagcggacc gtccgtgata ctactgactt tgccggcacg ccaatcatgt tttctttccg
     4621 ggacgagaag caaattaagg ccaatcgtga caagcaaaag gaaagtagca aagactaaag
     4681 gtttacaata aaaatagggc gggtgacgca tcgattactc gacacatcac ccgcccatat
     4741 gaccgaagcc ttagacgact cggtcattcc ccggatcccg atcgcttgcc cagaaccagt
     4801 tctgatttcc gagacaaact cgcttatcac cgttgcgacg gtgacgcttg tactcagcag
     4861 cctgccagag gccgtcgata agcgcggcgg cggccaaaag accgaagcct accgtaatcc
     4921 aagtcagaag tcgcgtcatc cagatgtcgt aaccgccgaa cgtctggtag ctgcccacca
     4981 ggacaaccga ccaaaagacg aaaatcacca ggaaaaagcg acgatcctga actgaaaagt
     5041 gcttcatgct cgactcatac tcgggcatta ctactccttt ggggaagaag aggcggagtt
     5101 gcctcatagt tttatatatt aacataagtg atatattttg tcaatataat ttactctgtc
     5161 gtatactgtt aaatatgaaa gtaatcttgg gtcaaggcaa tccaggcgct aactatgacg
     5221 gctcgcgtca caatatcggt ttcgccgtgg taga
//
LOCUS       DASSBW010000082         5926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1305815, whole genome shotgun sequence.
ACCESSION   DASSBW010000082 DASSBW010000000
VERSION     DASSBW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305815"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFG56_02330"
     CDS             complement(<1..887)
                     /locus_tag="VFG56_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6622750.1"
                     /translation="MNKVASYLQQHLSGEVLSSESAREFFSTDASILQQKPSLVVYPR
                     STNDIRKVARFTWQLAEKGHVMPITARGSGTDLSGAAIGRGVIMVMPAHLNRILEIDT
                     KQRLVRLQPGVNFKSLQETLTTHGLFLPAYPTSYEYSTVGGAIANNTAGEKSLKYGVM
                     RDYVERLEVILANGEVIETSPLNKKQLEHKKGLATMEGELYRAVDGLLSDDPTLIDKL
                     ILNVSKNSMGYALDQVTAKKGMFDLTPLFVGSQGSLGVISEAILRLEPYSPKADLIMA
                     TFSDFENALDAIDKLTSLAP"
     gene            complement(1258..1346)
                     /locus_tag="VFG56_02335"
     tRNA            complement(1258..1346)
                     /locus_tag="VFG56_02335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1310..1312),aa:Leu,seq:gag)
     gene            complement(1679..3493)
                     /locus_tag="VFG56_02340"
     CDS             complement(1679..3493)
                     /locus_tag="VFG56_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6622751.1"
                     /translation="MAEENKPEASQWRRFAKLKPTSKNLSKRARKIEKTTLRHAHRFI
                     VKRWDNAQDVRRHIVGWLALVVLLIAAALLQGGWWYPSAYSIKAPATGTTYAEGVIGQ
                     LDSINPLFAQTPSELSASQLVFSGLLDYDSAGQLRGDLAASWQAVEDGEAYVVKLKPD
                     LKWQDGVTLNADDVVFTVNLMQDQRVKSNYYKSWSNIDISKIDDLTVKFDLPGSYSPF
                     PSALTFGILPKHLLENVSPDSLRESDFALNPVGSGPFAFQSKQVIDANNDRLVVQLRA
                     NPYYYRGQPRLQSFQLHVYGDVEQLKQGYLSGQVNAISAAQLSSEELDKLSQLRGDSV
                     QSHADLNDGVFALFNLDNPPLDNHDIRAGLLAATNRPAIIDALHGNASRLSGPLLPNQ
                     FEAKNPSHQAPYSQKAALASFAKAGWKLNSNHVLKKGQAKLSLSLVAPNSGDYPVVAN
                     RIADDWRAVGVDVAVDLVDPKTIVQNVLSPRGYDVLVYELSLGSDPDQFAYWDSSQAN
                     PDGLNLSNYQSDVTDEALETARAKFDPIVRSDKYNVFFNQWIKDIPAIALYRPTLHYL
                     STKNTTTISPNGTVINQPDRYRNVIYWSVGKRAVFTTP"
     gene            complement(3504..3722)
                     /gene="secG"
                     /locus_tag="VFG56_02345"
     CDS             complement(3504..3722)
                     /gene="secG"
                     /locus_tag="VFG56_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET6622752.1"
                     /translation="MNQILDIVMIASAVLMILCILLQSRGASLGAGFGASGELFTARR
                     GIDKNLHETTIVLVVVFVLSIILNLVFA"
     gene            complement(3719..4108)
                     /locus_tag="VFG56_02350"
     CDS             complement(3719..4108)
                     /locus_tag="VFG56_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6622753.1"
                     /translation="MRKQLAVFLLRLVMNAFGLWVAAHLLTGHITDTGATLVSFLLAG
                     LILSVANTLLRPIVIILSLPAILLTLGLFMLVVNGFMVYLALNLLPSISISFPSAVLA
                     AIIIGLINYVISGIIEISERPKGANIA"
     gene            complement(4148..5350)
                     /locus_tag="VFG56_02355"
     CDS             complement(4148..5350)
                     /locus_tag="VFG56_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET6622754.1"
                     /translation="MTRHINSDFFTNNRRRLLESLPDKALVVMAGHREMQRAADASFK
                     FEQDASFYYLTGVVTPDWLLVIDKDSGSDYLVAPDLDPVKLLFDGGLSAEAASRVSGV
                     NSVIEASELVTRLDKYKQRGRTIYCPLTPAHLKTTGFVINPAADKLARNLRRQFGEVH
                     DLTQALSRLRAIKQPAELSAIEEAIAATARAFNHIKDHLDDYDYEYQVEAEMTRLIRA
                     SGARGHAYDPIVASGKNACTLHYIDNDQLLAPDQLLLLDVGARSASGYAADISRTYAV
                     QPNSRQRQVYQAVAKAQAECIDLLKPGLKLVDYQRSVDKFMRSAIKSLGLSTFRYRDY
                     FPHAIGHGLGLDVHDPLGGYDSLQPGMVMTVEPGVYIAEESIGVRIEDDILITESGHD
                     NLSAAIKY"
     gene            complement(5347..>5926)
                     /locus_tag="VFG56_02360"
     CDS             complement(5347..>5926)
                     /locus_tag="VFG56_02360"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET6622755.1"
                     /translation="ETIKRRLSEKNLNQWGKPNLVLIDGGKGQLDAALKARATSGQTM
                     PFIGLAKREEQIVIKKSTDDSPGSSVQSPASNVYLNYDVLKKLGGYTTESEDFILVNL
                     PHSTNLIKLLQRIRDESHRFAVSYHSSLKTKRQTSSRLEDVPGIGPVTRRQLIKKFGS
                     LRGVTAASQAELAEVVGRAKAVIIKQSLDIDK"
BASE COUNT         1494 a         1520 c         1431 g         1481 t
ORIGIN      
        1 ggtgctaatg aagtgagctt atcgatagcg tccagggcat tttcaaagtc ggagaaagtc
       61 gccatgatca gatcggcctt cggactgtac ggctccagac gaagtatggc ctcactaatg
      121 acacctagac tcccctgtga tccaacaaac agcggcgtca ggtcaaacat acctttctta
      181 gccgtgactt ggtcgagtgc atatcccatg ctatttttgc tcacatttag gattagcttg
      241 tcgatcagtg tcggatcatc ggacagcaaa ccgtcaaccg cccgataaag ctccccttcc
      301 atggtggcta gacctttttt gtgctccaat tgcttcttgt tgagcgggct agtttcgatc
      361 acttctccgt tagctaggat gacttccagc cgctctacgt aatcccgcat cacgccgtat
      421 ttgagcgact tctccccggc ggtattattg gctatagcgc caccgacagt agaatactca
      481 tacgaagttg gataagccgg caggaaaaga ccgtgagtag tgagtgtctc ctgcagagac
      541 ttgaagttta cgcctggttg taggcgcacc aagcgctgtt tagtgtcaat ctctaagata
      601 cggttcagat gtgccggcat gaccatgatg acaccccggc cgatagccgc acccgatagg
      661 tccgtaccgc taccgcgtgc ggtaataggc ataacatggc ccttctcagc cagctgccaa
      721 gtaaaacggg ctactttcct gatgtcattg gttgagcgag gatatactac taaactaggc
      781 ttttgctgca agatactggc atcggtcgag aaaaactcac gggctgattc gctagagagg
      841 acttcgccac tcaaatgctg ctggagatag gaagcaactt tgttcatttt atctatatat
      901 tccctttcat acgataatac cataagcggc gtatcagacc gtaaactaaa aacagatgca
      961 aaagcatctg tttttaactt ctcgttcgct ttgctcactc aaacggaagt tctgcgtctt
     1021 tccgacggac caaaccaaat aaaaagcgga tgcaggagca tccgttctct atttggtgcg
     1081 ccggagaaga ctatcggcct aaaggcctcc tccgctcaag ctttctcgga atcaattcct
     1141 gcgagagctt tcgcttcgcg acttctcgtt cgcttcgctc actcaaacgg aagttctgcg
     1201 tctttccggc ggaccaaacc aaataaaaag cggatgcagg agcatccgtt ctctatttgg
     1261 tgcgccggag aagacttgaa cttccacgac ctaaagcggt caccagcccc tcaagctggc
     1321 gcgtctacca attccgccac cggcgcataa aaccctacct ttaaatgtac ttgcacctag
     1381 cttaccgaga cttgaggcct caagctgggt cacccgtcga ttgctaaagc aatcgcgctc
     1441 gaccccgcct cagtcggtga gctaaagctc agccggcttc cggccttgcg agctgtcacc
     1501 ggcagctctc acgtctacca attccgccac cggcgcataa tttagatttg aggtacctcg
     1561 tagcctatcg ctttttacag atttcgcgcc gcgcgtctac gattcactga cgtttcacgc
     1621 agccgccacc ggcgcatacg agacagacta tattttacca gacaacatca ggtaagtact
     1681 aaggggttgt aaaaaccgct cgcttaccaa ccgaccaata gatcacatta cggtagcgat
     1741 caggctgatt gataacagta ccattgggac taatggtggt ggtattttta gtcgacagat
     1801 aatgcagggt tggccggtac agggcaatag ccggaatatc cttgatccac tgattaaaga
     1861 agacgttgta cttatccgag cgcacgattg gatcgaattt agctcgagct gtctccaagg
     1921 cttcatcagt cacgtcagat tggtaattgg acaggttgag tccatccgga ttagcctgtg
     1981 aagagtccca gtaggcaaat tggtctggat cggatcctaa tgacagttca tagaccagta
     2041 cgtcgtaacc gcgagggctg agtacgtttt ggacgatagt cttggggtct actaaatcca
     2101 ccgccacatc aaccccaacc gcccgccagt catcggctat acggttagcc actaccgggt
     2161 agtctccgct attaggtgcc accaaactta agctgagctt ggcttgaccc ttcttaagga
     2221 cgtgattgga gtttagcttc cagccagcct tggcaaatga agctagcgcg gctttttggc
     2281 tatacggtgc ctggtgagat ggattcttgg cttcaaactg attgggcagc aagggaccgc
     2341 tcaagcggct agcgttacca tgaagggcgt cgataattgc cggtcgatta gtcgccgcta
     2401 acaatccagc tcggatatca tgattgtcca aaggaggatt atcgagattg aacaaagcaa
     2461 agaccccgtc attgaggtca gcatgcgact ggacgctatc accgcgcaat tggcttaatt
     2521 tatctagttc ttcactcgac aactgagccg cgctgatggc attgacctgg ccactcaaat
     2581 agccctgctt gagctgctcc acatcgccgt agacatgtag ctggaatgac tgaagcctcg
     2641 gttggccgcg gtaatagtag gggttggccc ttaactgaac caccagacgg tcattattgg
     2701 catcgataac ctgtttggac tggaaggcaa acggtccgct gccgaccggg tttagagcaa
     2761 agtcactttc acgcaggcta tcaggactga cgttctcaag caggtgtttc ggcaatatac
     2821 cgaaagttaa agcgctcgga aacggtgaat agctgcccgg tagatcaaac ttaacggtta
     2881 gatcatcaat cttactaatg tctatattgg accagctttt gtagtaattg gattttaccc
     2941 gctggtcttg catcagatta acagtgaaga cgacatcgtc cgcgttcagt gtcacaccgt
     3001 cttgccactt aagatcgggc tttagtttaa ctacgtaagc ctcgccgtct tcgaccgcct
     3061 gccagctagc agctaagtcg ccgcgcaatt ggccagccga atcatagtcg agtaagccag
     3121 agaaaaccag ctggctagcc gatagttccg atggtgtctg agcaaacaac ggattgatgg
     3181 aatctagctg gccgataaca ccttcggcat aagtcgtgcc ggtagctggt gccttgatgc
     3241 tataggccga cggataccac cagccaccct gtagtaaagc agcggcaatc aacagtacga
     3301 ctaacgccag ccaaccgacg atgtgccggc gaacatcttg ggcattatcc caacgtttga
     3361 cgatgaagcg atgagcgtgg cgcaaggtgg ttttttcaat cttgcgggct cgcttagaca
     3421 agttctttga ggttggcttg agcttagcga accgccgcca ctgagaagct tctggcttgt
     3481 tctcttccgc cactttaatc cccttaggca aaaactaaat tcaagattat cgacaaaacg
     3541 aagactacta ccagaacgat ggttgtctcg tgcaggttct tgtcgatgcc tcgacgggct
     3601 gtaaacaatt cacctgaagc accaaaaccg gcgcctaaag acgcgccacg tgactgcagc
     3661 aatatgcaca aaatcatcaa aacagccgag gcgatcatga cgatgtctag gatctgattc
     3721 atgctatgtt agctcctttt ggtctttcac tgatctctat tataccgctt atcacataat
     3781 tgataagacc gatgataatg gctgctagga cagcgcttgg gaaacttata ctgatgctag
     3841 gtaggagatt gagcgctaga taaaccatga agccattgac cactagcatg aacaagccta
     3901 gggtcaacaa aatggccggt aagctgagga tgataacgat cggtcgaagc agcgtattgg
     3961 ctactgacaa gatcaaaccg gccaacaaga aactgaccaa agtggctcca gtgtcggtga
     4021 tatggccagt cagcaagtga gctgctaccc acaagccaaa ggcgttcata accaaacgta
     4081 ataaaaatac ggctaactgt ttgcgcataa ctacttagtg ataattatac cttattgtca
     4141 gtccaagtca gtacttaatt gcagccgata agttatcgtg gccactttcg gtaatcagta
     4201 tgtcgtcctc gattcgtacg cctatggatt cctccgctat ataaacacct ggttctactg
     4261 tcataaccat gccgggttgc agactgtcgt agccgccgag cggatcatga acatccagac
     4321 ccaagccatg accgatagcg tgagggaaat aatcgcggta tctgaacgta gacaagccga
     4381 gggattttat agccgatcgc ataaacttat cgactgaacg ttgataatca accaacttta
     4441 agccaggttt aagcaaatca atgcactcgg cttgagcctt ggccaccgcc tggtagacct
     4501 gacgctggcg actgtttggt tggactgcgt aggttcgact gatatcggca gcgtagccac
     4561 ttgcagatcg agcaccaaca tcaagcagca gcagctgatc aggcgctaga agttggtcgt
     4621 tgtctatgta atgaagtgta caggcatttt taccactagc cacaatcgga tcgtaagcat
     4681 gaccacgagc accactagcc cgaattagcc gtgtcatctc ggcttcaacc tggtactcgt
     4741 aatcataatc atctaggtga tcttttatat gattgaacgc tctggccgta gctgcgatag
     4801 cttcttcgat agccgatagc tcggccggct gcttgatcgc ccttagccgt gacagcgctt
     4861 gggttaagtc gtgaacttcg ccgaattgtc gtctgagatt tcgggctaac ttgtcggcgg
     4921 cagggtttat cacaaaaccg gttgtcttta gatgggccgg agtcaacggg cagtagatag
     4981 ttcgaccacg ctgcttgtat ttatccagtc gggtgactaa ttcactcgct tcaatcacgc
     5041 tattgactcc gctgacccgg ctagccgctt cagccgacaa gccgccgtcg aatagtagtt
     5101 tgacggggtc caaatcagga gctacaagat agtcgctacc ggagtccttg tcgataacca
     5161 gcagccaatc tggtgtcacc actcctgtga ggtaataaaa cgaggcatct tgttcaaact
     5221 taaagctggc atcggcggcg cgttgcatct cacgatgtcc cgccataacc accagggctt
     5281 tatccggcaa actttctagc aaccggcgac gattgttggt aaaaaaatca gaattaatat
     5341 gacgcgtcat ttatctatgt ccaggctttg ctttattata accgccttag cccgaccgac
     5401 gacttcagct aattcagctt ggctagccgc cgtaacgcca cgcaagctac caaacttctt
     5461 gattaactgg cggcgggtga ctgggccgat gccaggaaca tcctccaggc gagaactggt
     5521 ttgtcgcttg gttttcaagc tggaatgata gctgacggca aatctatgtg actcgtcacg
     5581 tattcgctgt agtagcttaa tcaaattggt agaatgcggc aaattaacca gtataaaatc
     5641 ctctgattcg gtagtataac cgccaagttt ttttagaacg tcgtagttta aatagacatt
     5701 ggacgctgga gattggacgc tggaacctgg actatcgtct gtagatttct ttatgacaat
     5761 ttgctcttct cgcttagcca ggcctataaa cggcatagtc tgtccgcttg tcgcacgagc
     5821 ctttaaggca gcgtcaagct ggcctttacc gccatcgatc agcacaaggt ttggtttacc
     5881 ccactgattc aagttctttt cgctcagacg tcgtttgatc gtctcg
//
LOCUS       DASSBW010000083         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_917431, whole genome shotgun sequence.
ACCESSION   DASSBW010000083 DASSBW010000000
VERSION     DASSBW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917431"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VFG56_02365"
     CDS             complement(<1..1539)
                     /locus_tag="VFG56_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622756.1"
                     /translation="MNRRILGPAVVALAIMAVILGGLIWLLAASHSQKDDDSSSISKL
                     SGADNLQFGELDLTPSKEIVGGDLAVTGQLTVSDGLSSSSLVITPSDPTSPAVGQLYV
                     TSDNNLHYFDGSDDIVLSQIGQLDDLATQVGQLNGLANQVGQLNGLSGQLGTLQQLLD
                     AQSDRIADNEARVTSLQGQTGDLQINSGPGIDVSGTTISNTGVLSCQGRIGDVTLTAG
                     SGIAVNGTTVSNTGLTGLNGLTGGVVIQGTAGQLAVVKSGQNLRFQLDPSVSLLGQTI
                     SNSELENSSLAVFAGNGLIGSGVASLGGGVTVSVGAGDGISVSNDTIAVDSTVCRTSG
                     NCVGTGGAGSAIGGSGTTGQIAVFDSPGTIASSIISQNLAGDTITVTGTLTATALQGD
                     GSGLTNVDALTLQGEGKSYFTDAGNLASGTLSDARLSSNVAFYNAGTANFTGTLQQNG
                     DPVCTTAGNCGGITGSGGLNYLTKFDSSGNLVTSLIYDDGTSLGVGTTTPGYLLDVQG
                     GTGVV"
     gene            complement(1539..2327)
                     /locus_tag="VFG56_02370"
     CDS             complement(1539..2327)
                     /locus_tag="VFG56_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622757.1"
                     /translation="MMWLKQSGSVWGIGYLMLCFFATVLLVPSASAYRATEYQIDQDW
                     QVGSLVALDDEGQPILASLGNQNYIGVVVSNSDGSVQIADEASSIPMLVSRQTGDIKA
                     GQRLGLSSVAGVAVKWQPGSPLIAVAKQSAEDWQTSQVVSDDGRSEEVSLSLVNVQLI
                     KASGANQAGDSSYMNAIQSAANQIAGHSVAIWQISVALVLGLGGLILSLGLLFISSRE
                     SFFSIGRNPLAGNTIMGGLWKMSAVSVVIMLISLSSAYLILRIG"
     gene            complement(2796..4136)
                     /locus_tag="VFG56_02375"
     CDS             complement(2796..4136)
                     /locus_tag="VFG56_02375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6622758.1"
                     /translation="MSQGIVRSVSSLSAEIEFYDSPPQPGEVLKVDNEAGSQLLVENS
                     SAGNLVLGLKLEFDNSIQKGMAVISTGRGLEIPVGEDMIGRIFDAFGRPMDGGQPAEA
                     KTSRNILAPKPRPINVGGKKLQVLETGIKVIDFFAPFVKGRKTGIVGGAGVGKTVLIM
                     ELIHNIAKNDDGLSIFTGIGERIREGHELHDTLSERDLLKNTCLFFGQMNETPIQRSL
                     VGPAAATVAEYFRDEHSKDILFFADNMYRYVQAKNELSTVLKQIPSEGGYEPTIFSDV
                     KTLQNRLSSSDKGSITAVQTIYVPADDLSDPAVQMIQNELDSTIVLSRKIAEQGIRPA
                     VDLIRTSSSMLTPDVVGERHYSLSLKVQATLQKYESLRSIVAIVGETELSQEDRADYG
                     RALRLIQYFSQDMSVTEDLNGRKGEYFSLEETLSGVESILSMGAPSEQVTNTAN"
     gene            complement(4133..>4981)
                     /locus_tag="VFG56_02380"
     CDS             complement(4133..>4981)
                     /locus_tag="VFG56_02380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET6622759.1"
                     /translation="DAMSQLQDILKDKEAMDSVALLTSAFEGIASMHVSRIKTRVERS
                     EAFFAELWELYSQLIVDKDFHVEHHQPKYDRQLILIVTSEGSLSGDIDHRLIDSVLNN
                     YNSEKQSLSVIGHHGAGLLQQRGVAFESVYRMPDRDDEFKAEDLLIEVQQYQSCTVYY
                     QAYESLMVQGIRQVELNKAVQERGNKQERGDNYISQSNYIFEPSVSSVVEHMERSMKG
                     ITINEVILSSQLAQQASRFRAMSAARSRARRAAGELGAKLSYTKRTVKDERAREIVSG
                     LRGASL"
BASE COUNT         1237 a         1280 c         1164 g         1300 t
ORIGIN      
        1 tacaacgccg gtaccaccct gaacatcaag taggtaacca ggagtggtgg tgcctacacc
       61 taaactagta ccgtcatcgt atataagtga tgtaaccaag ttgccgcttg aatcaaactt
      121 agttagataa ttaaggccgc cagagcccgt aatacctcca cagttgccgg ctgtcgtaca
      181 aacagggtcg ccgttttgct gaagtgtccc ggtgaagttg gctgtaccgg cgttataaaa
      241 agctacattg gaactcaacc tggcatcgct caaggtgccg gaagctaggt tgcccgcatc
      301 ggtaaagtag ctcttaccct caccctgaag agttagtgcg tcgacgttgg tcagtccaga
      361 accgtcaccc tgcaaagcgg tggcagtcaa ggtaccagtg acggtaatgg tatcaccggc
      421 aaggttttgc gaaatgatag acgatgcgat agtacccggt gagtcaaaca cagctatctg
      481 tccagtcgta cctgatccgc caatagccga accagcaccg ccggtaccga cacagttgcc
      541 gctagtccga caaactgtcg agtcgacagc tatagtatcg ttcgatacag atataccatc
      601 gccggcaccg acacttacgg taactccgcc gcccaagctg gcgacaccgc tgccgataag
      661 accattaccg gcaaatacag ccagactgga gttctccaat tcgctattgc taatggtctg
      721 accgagcaag ctaacactag gatcaagttg gaagcgcaag ttctggcccg acttaaccac
      781 tgccagttga ccagctgtgc cctgtataac caccccaccg gttaaaccat ttaagccggt
      841 cagaccggtg ttagataccg ttgttccatt tacagctata cctgaacctg ctgtcaaagt
      901 aacatcgccg attctaccct gacaacttag tacgccggtg ttgctaatcg ttgtgccaga
      961 tacatcgata cctggaccgg agtttatttg caaatcgcca gtctgacctt gcaggctagt
     1021 tacccgagct tcattgtcgg ctatgcggtc ggactgggcg tccagcagtt gttgtaaggt
     1081 acctaactgg ccggaaagac cattgagctg gcctacctga ttggccaggc catttagttg
     1141 accgacctgg gttgctaagt cgtctagctg accgatctgg cttaagacga tatcatccga
     1201 tccgtcgaag taatgaaggt tgttgtcgct agtcacataa agttggccta ccgctgggct
     1261 ggtcggatcg gacggcgtaa ttaccaacga cgagctggat aagccgtcgc taacagtcaa
     1321 ttgaccggta acagctaggt cgccaccgac aatttctttg gagggagtta ggtcaagttc
     1381 accgaactgc agattgtcgg cgcctgagag cttagagatg gaactcgagt catcgtcctt
     1441 ttgtgaatgc gaagccgcca acagccaaat cagtccgccg agtatcaccg ccatgatggc
     1501 gagggcgaca accgctgggc caagaattcg ccgattcatc accctatcct caagattaga
     1561 taagcactag ataagctgat tagcatgatc acaaccgaca cggccgacat cttccatagg
     1621 ccgcccatga tggtgttacc ggcgagtgga ttgcggccga tagagaagaa gctttcgcga
     1681 ctcgatatga acaacaggcc cagtgataag atcaagccac cgaggccgag aaccaaggcc
     1741 accgaaatct gccatatggc cactgaatga ccagctattt gattggctgc actctggatg
     1801 gcgttcatgt acgacgaatc accggcctga ttagcgccgc tggcttttat gagctggacg
     1861 ttaaccagtg ataggctgac ctcttcactc cgtccatcgt cgcttacgac ctggctggtc
     1921 tgccagtctt cggctgattg cttggctact gcaattagtg gactacccgg ctgccacttc
     1981 acagcaacac cggcgacgct ggaaaggcct agtcgctgac cagccttgat atctccagtt
     2041 tgacggctaa ccagcattgg aatcgaactg gcttcgtcag caatctgaac tgatccatca
     2101 ctgttcgaca ccactacgcc gatgtagttt tggttgccta gactggccaa gatcggttgc
     2161 ccctcgtcat caagtgccac tagcgaaccc acctgccaat cttgatctat ttggtattcg
     2221 gtagctcgat aagccgatgc agatggaacc agcaagacgg tagcaaagaa acacagcatc
     2281 aaatagccta tcccccaaac gctaccgctt tgtttcaacc acatcatttt gttttataag
     2341 tttctcttta cttatttaca gccgattata ggtagctaac agataaatag caaggctttt
     2401 gaacaaattt tttgtctcaa aattggattt tagctagcct atgaatcttg atataaaatt
     2461 tgatgtactg aagtctacaa tacatcaatg ttttgtgctt atgcttatct caagaaatat
     2521 gtatattgtt gttagtatta cactgaattt agatgtcaag aaagactgtg acactatcgg
     2581 attttattct tagtaaacca ccgcttattg taattttttt tgtctgtccg tcgggcaatt
     2641 taagttctat gtcgccggtt gtcagaagcg taatgaagtt atggtgctcc ggtaaaacat
     2701 caaatttgcc gacattattg gtagccgata aactatcagc ttccccctca aaataaaccc
     2761 ggttgggtga taggacctta accttcaggg ttttatcagt tggctgtatt ggtgacttgc
     2821 tcacttgggg cacccatgct taggatcgat tctacgccgg ataaagtctc ttccaggctg
     2881 aagtattcac ccttgcggcc atttaagtct tctgtaaccg acatgtcttg cgaaaagtac
     2941 tggatcaatc tcagtgcacg tccgtaatca gctcgatctt cttgactaag ctcggtctcg
     3001 ccgacgatag ccacaatact cctcaaggac tcatactttt gcagggtggc ctgaacctta
     3061 agactcaggc tatagtgtcg ttcgccaacg acatccgggg ttaacatcga agaagatgtc
     3121 cttatcagat cgactgccgg tctgatacct tgctcggcta tcttacgact aagaactata
     3181 gtcgaatcaa gttcattttg aatcatctga acggccggat ctgataggtc atcggccggc
     3241 acgtagatag tctggactgc agttatagag cctttatcgc ttgagcttag gcggttctgt
     3301 agagttttaa catctgagaa gatggtcggc tcatagccgc cttctgacgg tatctgcttg
     3361 agtactgtcg atagctcgtt cttagcttgg acatatcgat acatattgtc ggcaaagaag
     3421 aggatatcct tagagtgttc gtctcggaaa tactcggcta cagtggcggc agccgggccg
     3481 accaaagatc tttggattgg ggtttcgttc atctgaccga agaacagaca agtgttcttg
     3541 agcaagtcgc gttcactcaa ggtatcgtgc agctcgtggc cttccctgat ccgctcacca
     3601 atacctgtga aaatactgag gccatcatcg ttcttggcga tattgtgaat caactccatg
     3661 atgagtaccg tcttaccaac accagcgcca ccgacaatac cggttttgcg gccttttacg
     3721 aacggtgcaa agaaatcaat caccttgatg ccggtttcaa ggacctgtag cttcttgccg
     3781 ccgacgttta ttggacgtgg ttttggtgcc aggatgttgc gactagtctt agcctctgcc
     3841 ggctgaccgc catccatagg acggccgaag gcgtcaaaga ttcgaccaat catatcttca
     3901 ccgactggga tctccaaacc tctgccagta gagatcaccg ccataccctt ttggatagag
     3961 ttatcgaact ctagtttaag gccaagtact aggttgccgg cgctactgtt ttcgaccaat
     4021 agctgactac cagcctcatt gtcgaccttt aagacttcgc cgggctgagg cggactgtcg
     4081 tagaactcga tttcagccga caaagagctg accgagcgaa caataccttg gctcatagac
     4141 tagcacccct taagccgctg acgatttccc gggcccgctc gtctttgacg gttcgcttag
     4201 tgtagctcag cttagcccct agttcaccgg cggctcgacg cgcccgactt cgggctgccg
     4261 acatagccct aaaccggcta gcctgttgag ccagttgtga gctcagaata acttcattga
     4321 tcgtgatgcc cttcatcgat ctttccatgt gttcgacaac actcgagaca cttggctcaa
     4381 aaatgtagtt agattgactg atgtaattat ctccacgctc ttgtttgtta cctctttctt
     4441 gtaccgcctt attgagctcg acttggcgaa taccctgaac cataagagat tcataggcct
     4501 gatagtagac tgtacaactt tggtactgtt gtacttcaat caacaagtct tctgccttaa
     4561 actcatcgtc tctgtcaggc atacggtaaa cactttcgaa agctacgcca cgctgttgta
     4621 ataagccggc accgtggtgg ccgatcaccg aaaggctttg tttttcagag ttatagttat
     4681 ttagaacgct atcgatcagc ctgtggtcga tatcaccgct taaagaccct tcagaagtaa
     4741 cgatgagtat tagctggcga tcatatttgg gttgatgatg ttcgacatgg aagtctttgt
     4801 cgacgatcaa ctgcgagtac agctcccata actccgcaaa gaaagcctcc gaacgttcca
     4861 cgcgggtctt gatcctagat acgtgcatac tggctatacc ctcaaaggca ctagtcaata
     4921 gtgcaactga atccatagct tctttgtctt tcaatatgtc ttgaagctgg ctcatagcgt
     4981 c
//
LOCUS       DASSBW010000084         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1147250, whole genome shotgun sequence.
ACCESSION   DASSBW010000084 DASSBW010000000
VERSION     DASSBW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147250"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VFG56_02385"
     CDS             complement(<1..1251)
                     /locus_tag="VFG56_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622760.1"
                     /translation="MFRKLVENVPFSPALVGQLGFYAHRLKKEEATRRLGLVFTALAL
                     VVQSLAVFAPPESANAANPSDFIYGGVTSVHQVLEAYDRSARGNGDLKDIMDYVGITR
                     AELARMKSGSINSKGHGTGDRAWQTWGREHRFSAAKGEVKHIVPLDHGGNSTVYSKPL
                     WLYDHLPYTIKHGSTYPAFVGHSKKLGDFAIMKGCGNLVSTKTPKPAPKAHFLNATCS
                     VITGYAYDARNRDRNVKVYLYFGGVPGKGQRSPAIKASLKGNRFHYQVPAKYKKANHA
                     TKVWGVMVPLKGWGDSSVQFNNTTTIPGNCTKPQPTPTAQCLSLNIDQISRTEFKLIG
                     QADTGGGATIKSYRFAVTDSSGKSAYSKTINSSRLNAKTAKFALSQAGDYRAKLTIAT
                     SVGNQAGGDCIQSLTVVQPEICSLN"
     gene            1406..>2994
                     /locus_tag="VFG56_02390"
     CDS             1406..>2994
                     /locus_tag="VFG56_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HET6622761.1"
                     /translation="MAKQKTVSDYDASQIQVLEGLEPVRKRPGMYIGSTGYDGVHHLI
                     KEIADNCVDEAIAGHATFVDIKLLADGGVTVTDDGRGIPVDKHPKTGKSTLETVLTVL
                     HAGGKFGGGGYKVSSGLHGVGSSVVNALSTKFIAEVVKGGQLYRLEFETGVPQGALKK
                     VGPTDRPQGTSITFYPDATIFKETIEFDYKWVLDYLRHQAYLTKGMKARVEDERSGER
                     YAFYFDGGIKSYVKHLNIGKDILSDNIFYVERQVEDCMVEVAIQYNDSYVETVKAFAN
                     NVFNPEGGTHMVGFRSAMTRVINDYARKNSLLKEKEDNLTGDDIREGLTAVILVKLPD
                     PQFEGQTKNKLGNPEMRRYVEQVMNEYFAYYLEEHPASAKRIIGKAILAARARKAARA
                     ARDNVIRKGALDGLSLPGKLADCSSKDPKNSELYIVEGDSAGGSAKSGRDSKTQAILP
                     LRGKVLNVERARLDRMLNNNEIVSLIKALGVGIDEQFDIGGLRYDRIIIMTDADVDGS
                     HIATLLMTFFFRYMKEIVDGG"
BASE COUNT          739 a          700 c          814 g          741 t
ORIGIN      
        1 gttgaggctg cagatctcgg gttgaactac ggtaaggctc tgtatgcaat caccaccggc
       61 ttggttgcct accgaagtag cgatggttag tttagcgcgg tagtcaccgg cttggctcaa
      121 ggcaaactta gcagtcttgg catttaagcg ggagctgtta atcgtcttag aataagcgga
      181 tttgccactg gaatcagtta cggcgaagcg atagctcttt atcgtggctc cgccacccgt
      241 gtcagcttgg ccgattaact taaactcagt ccgactgatc tggtcgatat ttaaggatag
      301 gcattgggcg gtcggcgtag gctgcggttt agtacagtta ccgggtatgg tggtagtgtt
      361 attgaactgg actgaagagt caccccagcc ttttaacggc accatgacac cccatacctt
      421 ggtggcgtgg ttagctttct tgtatttggc tggcacctgg tagtggaagc ggttaccctt
      481 gaggctagct ttgatggctg gagagcgttg gcctttaccg ggcacaccac caaagtagag
      541 gtagaccttg acgttacggt cgcggttgcg agcgtcgtag gcgtaaccgg tgatgacgga
      601 acaggtggcg tttaggaaat gagcttttgg tgctggtttc ggtgtcttgg tgctgaccaa
      661 gttgccacaa cctttcatga tggcgaaatc acccagcttc ttggagtggc cgacgaaggc
      721 agggtaggtt gagccatgct tgatggtgta cggtaggtga tcatacagcc agagcggctt
      781 ggagtagacg gtggagttac cgccatggtc gagcggtacg atgtgcttga cttcaccctt
      841 ggccgcactg aagcgatgtt cacgtcccca ggtttgccaa gctcggtcgc cggtgccatg
      901 acccttggaa ttgatcgaac cggatttcat ccgagccagt tcagcccgag tgataccgac
      961 atagtccatg atatctttta agtcgccatt acctcgagcg ctgcggtcgt aggcctccag
     1021 gacctggtga acgcttgtca cgccgccata aataaagtcc gatggattgg cggcattggc
     1081 cgattctggc ggggcgaaga cggctaagga ctggaccaca agagctagag cagtaaaaac
     1141 cagacctagc cgtctggtgg cttcctcctt ttttagtcgg tgggcgtaga agcctagttg
     1201 gccgaccaaa gccggtgaaa atggtacatt ttcgaccagt ttcctaaaca tttttatttt
     1261 aaatctctcc tctattaaaa actttagcat aataaatatg ttcaatacaa tagaacttct
     1321 ttcccatctg ttacagcttg acctgagtac aaattagcag gtataataga agagatttca
     1381 agttagagta atttagtgag ggataatggc taaacaaaaa acagtgtctg actacgacgc
     1441 tagtcagatc caggtcttag agggtctgga accggtccgt aagcgaccag gtatgtatat
     1501 tggttcgacc ggttacgatg gtgtccacca tctgatcaaa gaaatcgccg acaactgtgt
     1561 cgacgaagct attgccggcc atgctacatt tgttgatatt aaactgctgg ctgacggtgg
     1621 tgtaaccgta actgatgacg gtcgcggtat tccggtcgac aaacacccta aaaccggcaa
     1681 gagtacccta gagaccgttc tgacggtcct gcatgccggt ggtaaattcg gtggtggcgg
     1741 ctacaaggtc agctccggtt tgcatggtgt tggctcgagc gtggttaacg cgcttagtac
     1801 taagtttatc gccgaagtag ttaagggtgg tcagctgtat cgactggaat ttgaaaccgg
     1861 tgtaccgcaa ggcgcactga agaaagttgg tccgaccgat cgtccacaag gaacttcgat
     1921 taccttttat ccagatgcga ctatctttaa agagacgatt gagttcgact acaaatgggt
     1981 actagactat ttgcgtcacc aagcttatct gaccaagggc atgaaggccc gagtcgagga
     2041 tgagcgcagc ggtgagcgct acgcctttta ctttgatggt ggtatcaaat cttatgttaa
     2101 gcaccttaac atcggcaaag acattctcag cgacaatatc ttttacgtag agcgccaggt
     2161 cgaagactgc atggttgaag ttgctatcca gtacaacgat agctacgtcg aaaccgttaa
     2221 agcctttgcc aacaacgtct ttaacccgga gggcggtacc cacatggttg gtttccgctc
     2281 cgctatgacc cgcgtgatca atgactacgc tcgcaaaaac tcactgctca aagaaaaaga
     2341 agacaatctg actggtgatg atatccgtga agggctaaca gcagtaattc tcgttaaatt
     2401 accagatccg cagttcgaag gtcagaccaa gaacaaactt ggtaacccgg aaatgcgtcg
     2461 ctacgtcgag caggtgatga acgaatactt cgcttattac ctagaggagc acccagcctc
     2521 ggccaagcgg attatcggca aggccatcct ggccgctagg gctaggaaag ccgcccgcgc
     2581 cgcccgtgat aacgttatcc gtaaaggtgc tctagacgga ctgagcctgc caggtaaatt
     2641 ggctgattgt tccagcaagg atccgaagaa ctctgagctc tatatcgtag agggtgactc
     2701 ggctggcggt tcagccaaat caggccgtga ctccaagact caagctatct tgccgctccg
     2761 tggtaaggtg cttaacgtcg aacgggcccg gttggacagg atgcttaaca acaatgagat
     2821 cgtgtctctc atcaaagccc taggtgtcgg catagatgag caattcgata tcggcggctt
     2881 aaggtacgat cgtatcatca tcatgaccga cgccgacgtc gacggtagtc acatcgcgac
     2941 attgttgatg acattcttct ttaggtatat gaaagaaatc gtcgacggtg gaca
//
LOCUS       DASSBW010000085         7620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_358148, whole genome shotgun sequence.
ACCESSION   DASSBW010000085 DASSBW010000000
VERSION     DASSBW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358148"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFG56_02395"
     CDS             complement(<1..372)
                     /locus_tag="VFG56_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622762.1"
                     /translation="MRGQNTIVINGKVYDAVTGLAVGAPDPVTPSVKSAVKKPSTGQS
                     LSGIQKPARGRQLVRKAQKSQTLKRQHLKKPATHKTPIHQARVPRAHQAVSRSHMISK
                     FAQPVEPTKPVEAATENQSTTI"
     gene            542..1906
                     /locus_tag="VFG56_02400"
     CDS             542..1906
                     /locus_tag="VFG56_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET6622763.1"
                     /translation="MVVSTLLISALCILLVLGYRFNLTKHTVTQGGLLQFDSQPASAN
                     IEVDGQAMSFKTPGKRNVAAGQHAVSMTLDGYRPWSKTVGVKAGQLVWLNYTRFVPTN
                     INTSSIKNFKNLSQLLASPDQEWLALWNDKKTAELVLVDISNSNQQEFTDIKLPSKVV
                     TGSKQQQFRLQEWDFGSRYLLIDHSFKGGHDFILLDRENPDQAVNLNNRFGLDIDSIH
                     FADTSGSSFYVMADGDLRRLDLANKTISAPLVKQAVSFTPYGDANLSYVAKTDSGYDV
                     GVLREGEVKQVAHYETKQTVVSSLTKYFGHNYLAVGVGDEVVIYKDPFSESVQPPLRR
                     IKLSFSISWLDYASNGRFLLAGNGDEFMTFDGDTKTIYQGSLDGIRQDNRPFLWLDDY
                     HFVSTGGERLRMIEFDGANPEFITTAEPGFDAALSSDGELLFSVVKHSDSYSLQASRL
                     VIEP"
     gene            complement(1903..3120)
                     /locus_tag="VFG56_02405"
     CDS             complement(1903..3120)
                     /locus_tag="VFG56_02405"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET6622764.1"
                     /translation="MQPEPPNDDRQLQVPSRSDSSARPDSRTAAADYMRREIDRLYEI
                     ENEEVSEPEPQATKSKPSVAPADTYKRQHSEAAAHTRKSENQAAYWKRYHSAWQSYYQ
                     QYYERYYLTQLRSKQSQPTSQVSTKPADPTEPAIVTGGDGLSRDQAVGELRQQLLGKV
                     STQAKKVRRSRHFVPLLSAVVIALTVLFLQYNNLIVAQVKAYVSPGSANPTSLIIDPT
                     TDFKVGPEPTVTIPKIGVEAPVVYDVTSLKESYIQNKLKDGVVHYPIPGASSVPGQNG
                     NTVIIGHSSNDVFNSGDYKFIFVQLDRLRKGDIFYLNYKGTRYTYSVTKSEVITPTQI
                     NKLVLPQTKPMATLITCTPPGTAWKRLVVYGEQISPDPAKAKPAQEPAGSDTKDISLP
                     TNSPTLLERLFGA"
     gene            3233..3319
                     /locus_tag="VFG56_02410"
     tRNA            3233..3319
                     /locus_tag="VFG56_02410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3267..3269,aa:Leu,seq:caa)
     gene            complement(3409..4278)
                     /locus_tag="VFG56_02415"
     CDS             complement(3409..4278)
                     /locus_tag="VFG56_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622765.1"
                     /translation="MSPEIISSYQRRLDRANRTGSFTEYCYTKANLEVEHAVQAAHQG
                     LIDDKQLEEVAVALRCSQEAAMDDGDVVTLLKSQLMEAFMPLIVYSEALNSHSLKPAE
                     ARHVRKNLDMDSAYDYLADIAQDGLTSYDNAKLITNDLAEADPSEIEVREHLKGFLQE
                     ITYLMLMTRHHTAKQYAVPALMYKDRLDPVDGRHVDAIYYDNRPTRRLKNCDVQIKSH
                     RELNWYYDYSVVVIDSVDLGNDAYSRLWLDYSGEFTTLRILLNEERQQASRADRHVLD
                     RIHQKVTRKVATV"
     gene            complement(4312..5403)
                     /locus_tag="VFG56_02420"
     CDS             complement(4312..5403)
                     /locus_tag="VFG56_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622766.1"
                     /translation="MGEPLNQNLEPCLDNAEERANRLMKMSAGFYFGGDQAIQSQQRI
                     IDGQLIEVAPHDGYRQYMNQAKIGSRVLEALDRPVSGANFPAVRAAAGWAHVEQAISR
                     KSLGFDQRLDAINQAQADFVKARDALGYIYDNYPEHWLVWPGTYWRYQLAIDVLPASL
                     AIADQRDSHTGQFDHQLIAESQANIRHTLGRIVRKYYQADAEQSRSKLQDGLDYNPNR
                     HEGRKNVLIGVGLEGAALYLTQLAADDRSISLPPSVRLDNAGRQGADLSLHQLKGTPY
                     TAQVKKRVRKPHTSRYTGTHLICGFHQMCLPGQPLIDTIAAIDADEHTGELTKMGRRM
                     IAIMRGPNQHGCSDVRQRLARPPERQIAV"
     gene            complement(5431..6225)
                     /locus_tag="VFG56_02425"
     CDS             complement(5431..6225)
                     /locus_tag="VFG56_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622767.1"
                     /translation="MTSLYWQKQSSSKPLFTDIEWNRPEQRARAGKLAIIGGSKLGFA
                     GVSEAYRSAREAGVGQIRVILPDTLKKTVPIDMLEVVFTATNKSGGFSRDSLDDIKAS
                     LSWADHGLLIGDAGRNSETAIIYEDLLSMTTPLTITRDAADLLRNSAASLVERDHTTL
                     VLSFAQAQRLFKDVYYPKMLAFSMQLTNLVETLHKFTITYPVTLVTFHQDQLIVAHDG
                     KVISQAFDEPMMIWRGITASTSSAYQIWSPNQPLEAIATSWLKLVK"
     gene            complement(6188..6931)
                     /locus_tag="VFG56_02430"
     CDS             complement(6188..6931)
                     /locus_tag="VFG56_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET6622768.1"
                     /translation="MRDRPSTKRRFAVFDIDGTLYRWQLFHELVEELVKQQALADPDG
                     LLGQTWNEWRGGKLSFHDYEHKLMHDVLYKNLVDVSVKAFDAACQAVVESSGHKVHKY
                     TADLLKDLQDQGYFTIAISGSQQELLDRFCVRYGFDIAVGALYERQQGRFTGRLERST
                     FGRKAEILKELVGQYNLTFTDSLAIGDSDSDIEILELVEQPIAFNPSEGLFDRAQTAG
                     WPIVIERKNMAYRLERNHDKLVLAKTIVL"
     gene            complement(6933..>7620)
                     /locus_tag="VFG56_02435"
     CDS             complement(6933..>7620)
                     /locus_tag="VFG56_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET6622769.1"
                     /translation="AKQGQAEVDLSGYEEYWNSAERPGYSGTAIFTKIKPISVSFGFS
                     DDIAERYDLAGDSYGDPTKEGRIITAEFDDFYVVNVYTPNSKGDLSRLDLRYKQWDPA
                     FLEHLVELQRSKKVLVCGDMNVAHQEIDLANPKPNIGKHGFTDQERQRFSEILAAGFI
                     DSFRHKHPDQHDAYTWWTHWANARTRNVGWRIDYWLASELLVLKLKAADIHPDVMGSD
                     HCPVSLTLEV"
BASE COUNT         1750 a         1797 c         2002 g         2071 t
ORIGIN      
        1 aatggtggtt gattgatttt cagtggcggc ttcaactggt ttggttggct cgaccggttg
       61 agcaaactta ctaatcatgt gagaacgact aacagcttga tgtgctcgcg ggactctggc
      121 ctggtggatc ggtgttttat gcgtggcggg ttttttcaag tgttgtcgct tcaatgtctg
      181 actcttttga gcttttctga ccagttgacg acctcgtgcc ggcttttgta tgccggacaa
      241 gctttgaccg gtcgaaggtt tcttaaccgc agatttaacg cttggagtaa cgggatccgg
      301 ggcaccgaca gctaggccgg ttacggcgtc gtaaactttt ccgttgatga caattgtgtt
      361 ttggcccctc attttgtccc gtctattttg catatagttt acggccgtct acagtaaaaa
      421 gcaacagtgc ataagcattt ttaagtgata taattgatga taactaaagt ttcctatgca
      481 caaacatcct cgtagtaaaa aacaccgcgc tcttatcatt cgctttttgg tatacggcgt
      541 tatggttgtg tctaccctgt tgatatcggc cctatgtata ttgttggtgc tcggttatcg
      601 gttcaatttg accaaacaca cggtaacgca aggtggcttg ttgcagttcg atagccaacc
      661 ggctagtgcg aacattgaag ttgacggtca agctatgtcg tttaagacgc ctggtaagcg
      721 caacgtagcg gccggtcagc acgcagtatc tatgacattg gatggttatc ggccatggtc
      781 aaagacagtc ggcgttaagg cgggacaact ggtctggcta aactataccc gttttgtacc
      841 gaccaatata aatacaagct cgatcaagaa cttcaagaat ctaagccaat tactagcttc
      901 acctgatcag gagtggcttg cgctgtggaa cgataaaaag acggcagagc tagttttagt
      961 cgatatctca aatagcaatc aacaggaatt taccgacatc aaattgccga gcaaagtcgt
     1021 gacggggtcc aagcagcagc aattccggct ccaagaatgg gatttcggat cgcgttatct
     1081 gttgatcgac catagcttta aaggtggtca tgacttcatc ttgctcgatc gggagaaccc
     1141 cgaccaagcc gttaacttga acaatcgctt tggtcttgat atcgacagca ttcatttcgc
     1201 cgacacttca ggcagcagct tctatgtcat ggctgacggt gatttacggc ggctagactt
     1261 ggcgaataag acgatttcag cgccactagt caaacaagct gtcagcttta ccccctacgg
     1321 tgatgccaat ttatcatatg ttgccaagac cgatagcggt tatgatgtcg gtgtactacg
     1381 cgagggtgag gtcaagcaag tcgcccacta cgagaccaag caaaccgtgg ttagcagttt
     1441 gactaagtac ttcggtcaca actacttggc ggttggcgtg ggtgatgaag tggtgatcta
     1501 caaggatccg tttagcgaat cggtgcagcc gccattgcgt cggatcaagc tgagctttag
     1561 tatcagttgg ctggactacg cttccaatgg acgctttctg ctggccggca atggcgacga
     1621 attcatgact ttcgacggcg acaccaagac tatctatcag ggtagcctag atggtattcg
     1681 acaagacaac cggccgttct tatggctaga tgactaccac ttcgttagta ctggcggtga
     1741 acgtttgaga atgattgaat tcgacggtgc taatcctgaa tttatcacta ccgcagagcc
     1801 tggtttcgac gcggcgctta gttctgacgg tgaactgcta tttagcgtgg tcaagcactc
     1861 tgacagctat agcctccagg cctcacgctt agttatagag ccttaggcgc cgaataaccg
     1921 ttctagtaac gtaggtgaat tagtcggcag gctgatatct ttggtatcgc tccctgccgg
     1981 ctcttgggcc ggtttagcct tggctggatc cggactgatc tgctcgccat agaccactag
     2041 gcgcttccag gcggtacctg gtggcgtaca agtgattaag gtagccatag gtttggtttg
     2101 aggcaagacc agcttgttga tttgggttgg cgtgataact tcgcttttag tcacactata
     2161 ggtgtaacgg gtacccttat agtttaggta aaagatgtca cccttcctca atcgatctag
     2221 ttggacaaaa atgaacttat aatcaccact attgaagacg tcatttgagc tgtgaccgat
     2281 tatgacggta ttgccatttt gacccggcac actcgacgct cccgggatcg ggtaatggac
     2341 cactccatct tttaatttgt tttggatgta actctctttc aaagaagtga catcgtagac
     2401 taccggtgcc tctacaccaa tcttcggaat ggtgacggtg ggctcgggac cgactttgaa
     2461 atcggtagtt gggtcgatga tcaggctagt cggattagcc gagccaggac taacataggc
     2521 ttttacttgg gcgacaataa gattgttgta ttgcaggaat aaaaccgtca aagcgataac
     2581 tacggccgag agtagcggca cgaagtgacg cgacctgcgg actttcttag cttgggtcga
     2641 taccttgcct agcagttgct gccttaattc gccgacagct tggtcgcggc ttaaaccatc
     2701 tccaccggtc actattgccg gctccgtcgg atctgccggt ttggtggaca cttggctagt
     2761 cggctgtgac tgtttgctgc gaagctgcgt caggtagtaa cgctcgtagt actgctgata
     2821 atagctttgc caagcgctgt gatagcgctt ccagtaagcc gcctggtttt cggatttacg
     2881 ggtatgagcg gctgcttcgg aatgctgacg tttgtaggta tcggctggcg cgacacttgg
     2941 tttagacttg gtggcttgag gttctggctc gctgacttct tcattctcaa tctcgtagag
     3001 acggtcgatt tcacggcgca tataatctgc cgcggcggtc cggctatccg gtcgagctga
     3061 gctatcgcta cggctgggta cttgtagttg gcgatcgtcg tttggcggtt cgggctgcat
     3121 ggttttaata atttctcact tttctaggta tattgtacaa ttgttgaggt cacgttacca
     3181 acgctagcta aggatgagtg gataagttgg tatacatttt ttaacatctc gtgccgcggt
     3241 ggcggaattg gtagacgcgc tagactcaaa atctggtgag cattagctcg tgccggttca
     3301 agtccggctc gcggtaccag atgttaaaaa atttgagttc gcgagcgaac aaaatttgcc
     3361 cggtctattt aagcttccac gttcgtagta ccggctagcg cggaatattt agacagtggc
     3421 aactttacgg gtaacttttt gatggatacg gtccaaaacg tgtcggtcag cacgagaggc
     3481 ttgctgtcgt tcctcgttta gtaggatccg cagagtggtg aattcaccgg aataatcaag
     3541 ccatagccgg ctataggcat cgttaccgag atcgaccgag tcaatcacta caactgagta
     3601 gtcataatac caatttagtt cacggtggct tttgatttga acatcgcaat ttttcagtcg
     3661 acgggtgggt cggttgtcgt aatagatggc atcgacatgg cgaccgtcaa ccgggtctaa
     3721 cctatcctta tacataaggg ccggcacggc atactgtttg gccgtatgat gtcttgtcat
     3781 cagcatcaaa taagtaattt cttgcaaaaa cccctttagg tgttcgcgga cttcgatttc
     3841 acttgggtca gcttctgcta agtcgttggt tatcagcttg gcattatcgt atgaggtcag
     3901 accgtcctgt gcaatgtcgg ccaggtaatc ataggcgcta tccatatcta gatttttgcg
     3961 gacgtgacga gcttcagccg gcttcagtga atgagagttc aaagcttcgg aatagacgat
     4021 taatggcatg aaagcttcca ttagctggct tttgagcagt gtgacgacat cgccgtcatc
     4081 catagctgcc tcctggctac agcgcagagc aacagccact tcttcgagct gcttgtcgtc
     4141 gatcaaacct tgatgagccg cttggacggc atgttcaacc tccaagttag ccttggtgta
     4201 gcaatattca gtaaagctac ctgtgcgatt ggcgcggtcg agccgtcgtt gatagctgga
     4261 aattatctcc ggggacattt tgcagattat aacataaaat gtagtgttta gctacacggc
     4321 gatttgccgt tcgggcggtc gggctaggcg ctgacgaaca tctgagcaac cgtgttgatt
     4381 aggtcctcgc atgatagcga tcattcttcg gcccatctta gtcagctctc cggtgtgctc
     4441 gtccgcatca atcgcggcaa tcgtatcgat caatggctgg cccggtaaac acatctggtg
     4501 aaagccacaa atcaaatgag taccggtgta acggctggtg tgaggttttc ttactcgctt
     4561 tttgacctgg gcggtgtatg gagtgccttt tagctgatgc aggctcaggt ccgcaccttg
     4621 cctgccggca ttgtcgaggc gtaccgatgg cggcagacta atggacctgt cgtctgccgc
     4681 aagctgcgtc agataaaggg ctgcaccctc taggccgaca ccgattagaa cattcttgcg
     4741 cccttcgtgg cggttgggat tgtaatcaag tccgtcttga agttttgatc tggactgttc
     4801 ggcatcggct tgatagtatt tgcgtacgat ccgaccaagc gtgtggcgga tgttggcctg
     4861 cgactcggct attagttgat ggtcgaattg gccggtgtga ctgtcacgct gatcggcgat
     4921 agccagactg gccggtaaca cgtcgatggc taactggtag cgccagtaag tgccgggcca
     4981 aactaaccag tgttccggat agttatcata gatgtatccg agagcgtcgc gagctttgac
     5041 gaaatcagct tgagcttgat tgatggcatc tagtcgctga tcaaacccaa gcgatttacg
     5101 gctgatagcc tgctcgacat gcgcccagcc ggcagctgct cggacagctg gaaagttggc
     5161 gccgctaact ggacggtcga gagcctctaa gacacgggag ccgatcttgg cttgattcat
     5221 gtattgccgg taaccatcgt gtggtgcgac ttctatcagt tgtccgtcga taatacgctg
     5281 ctgtgactgg atggcttgat cgccaccaaa ataaaaaccg gctgacattt tcatcagccg
     5341 gttagcgcgt tcttcagcat tgtctaggca tggctccaag ttttggttta gtggctctcc
     5401 catttactgc ttatggtaaa cctaaactgg ttatttgaca agcttaagcc agctagtggc
     5461 tatagcttcc aatggctgat ttggcgacca tatctggtaa gcgctactgg ttgaagccgt
     5521 aataccacgc cagatcatca taggttcatc aaaggcttgg ctgatgactt tgccatcatg
     5581 cgctacgatc aactgatctt gatggaatgt caccaatgtc accgggtatg tgatagtgaa
     5641 cttgtgtaag gtctcgacca agttggtcag ttgcatagaa aaagctagca tcttgggata
     5701 ataaacatct ttaaataaac gttgcgcctg cgcgaagctc agaaccagtg tggtgtggtc
     5761 tcgctcgact aagctagcgg cggagttgcg tagtaagtcg gcggcgtctc gagtaatagt
     5821 caatggcgtc gtcattgaca acagatcctc gtagataata gcagtctctg aattgcgacc
     5881 ggcgtcaccg atcagcaatc cgtgatcagc ccagctcaag ctggctttga tgtcgtccaa
     5941 gctgtcacgg ctgaagcccc cggatttatt ggtagcagta aaaactactt ccaacatatc
     6001 gataggcacg gtttttttga gagtatcagg taagatcacc cggatttggc cgacgcccgc
     6061 ttccctagct gatcgatagg cttctgatac gccggcaaaa cctaacttgc taccacctat
     6121 gatggctaat ttaccggcac gggctcgttg ttcaggtcta ttccactcga tgtcggtgaa
     6181 gagtggctta gaggacgatt gtttttgcca gtacaagctt gtcatggttt ctttctaggc
     6241 ggtaagccat gtttttgcgt tcgattacta tcggccagcc ggcggtttgg gcgcggtcaa
     6301 ataacccctc actagggtta aaggctatcg gctgttcgac tagctctagt atttcgatat
     6361 cgctgtcgct atcgccgata gctaaactgt cagtgaacgt gaggttgtat tggccgacca
     6421 gctcttttaa gatctcagcc ttacggccaa aggtagagcg ctctaatcgc ccggtgaagc
     6481 gtccctgctg gcgttcgtag agtgcgccta ctgctatgtc aaaaccatag cgaacacaaa
     6541 aacgatcgag taattcttgt tgcgagccgg agatggcaat ggtgaaataa ccttgatctt
     6601 gcaggtcttt cagtaaatcg gcagtgtatt tgtggacttt gtggccacta gattctacta
     6661 ccgcctgaca agctgcgtcg aaggccttaa ccgagacgtc gactaagttt ttgtagagca
     6721 cgtcgtgcat gagcttgtgt tcgtaatcat gaaagctcag cttgccaccg cgccactcat
     6781 tccaggtttg gccgagtaag ccgtcggggt cagctaaggc ttgttgcttt accagctctt
     6841 cgactagctc atggaacagc tgccagcggt aaagagtgcc atcgatgtcg aaaacggcaa
     6901 agcgacgttt agtggaaggc ctatctctca cgttaaacct ctaaggtgag actaaccgga
     6961 cagtgatcag accccatcac atcgggatgg atatcagcgg ccttaagctt caataccaac
     7021 agctcactag ccagccaata gtcgatccgc caaccgacat tgcgagttcg ggcgttggcc
     7081 cagtgagtcc accatgtgta ggcgtcgtgc tggtccgggt gcttgtgacg gaagctgtct
     7141 ataaaacccg ccgctagaat ttcgctgaaa cgctgtcgct cttggtcggt gaaaccatgc
     7201 ttaccgatat tgggtttcgg attggcgaga tcaatttcct ggtgagccac attcatgtca
     7261 ccgcaaacca agaccttttt tgagcgctgc agttcaacca aatgttcgag aaaggctgga
     7321 tcccattgct tatagcgaag gtctagtcgc gacaggtcgc ccttggagtt gggtgtataa
     7381 acgttgacga catagaaatc atcaaattca gctgtgatta tccgaccttc ttttgtcggg
     7441 tcaccatagc tatcaccggc cagatcatag cgctcggcaa tatcgtcgct aaagccgaag
     7501 ctgacactga tgggcttaat ttttgtaaaa attgccgtac cggaatagcc cggtcgctcg
     7561 gctgagttcc aatactcttc gtagcctgac aaatccactt cagcctggcc ctgtttggct
//
LOCUS       DASSBW010000086         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1585668, whole genome shotgun sequence.
ACCESSION   DASSBW010000086 DASSBW010000000
VERSION     DASSBW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585668"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFG56_02440"
     CDS             <1..271
                     /locus_tag="VFG56_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622770.1"
                     /translation="TSRDLTLEVADKVSYADVSQVINEALNNSQLLSSLELTHIYRAD
                     GGTTKNLTFRVQVIDPVKTITSDEANLLIEQVADQVKHKLGAKVV"
     gene            complement(313..924)
                     /locus_tag="VFG56_02445"
     CDS             complement(313..924)
                     /locus_tag="VFG56_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622771.1"
                     /translation="MNSATDDLGPPTEEMVPVNDELDATDEAAGLLEEQAGLFYGGKH
                     VKYLLKSIARSAGIAIATIAVFIVILIFTPSGVAYLQLTASLVLILVLIGCWWSLQRS
                     YIYWCDWRLRITAAIITIEFPTSRLFWLNYKSPMIPTRKIDTAELEKRTIAELFIFRN
                     SRTVTIDTAANEDEVFHNMSDMKDPELLKQVVEDLIIRIHGNY"
     gene            1083..2867
                     /locus_tag="VFG56_02450"
     CDS             1083..2867
                     /locus_tag="VFG56_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6622772.1"
                     /translation="MRILFKVLKSAGNLWPYYLAIAAFSVVLSLASLAIPFLIKAATD
                     LTVMSLQTGRAEVSGIVWIAVIILGVEVATTILSNFAGYFGDMMAAKLRQQLSERYYA
                     HLLKLPQSYFDRELTGTIINRLNRTIMEVTQFLNAFANNFFQMILMIILVLIIVGFYS
                     WQLAILLFVVYPIFLYLTMLTSRKWQVYQKQKNLETDVASGRFAEVVSSIRVVKSFIR
                     ESLEKREFSHHFSTTVDITKRQSSYWHKMDVSRRLVLNLIFFMVYAYIFVATVQSRFT
                     IGEMVLLIQLVNMVRMPIFSMSFIVDNAQRAIAGSVDYFEVMAIEPDLADKPAAQALK
                     VTRAAVTYDRVNFAYQSGQSVLKNLDFSIKPGEKVALVGESGEGKTTITNLLLRLYDP
                     NKGRIVIDDQDIANVTQASLRDNIAVVFQEPALFSGTIRENIAYGQPGATDKAIRAAA
                     KAANALEFIDKFELGFDTTIGERGLKLSGGQKQRIAIARAALKDAPILVLDEATSSLD
                     SRSEHLVQQALERLMKRRTTLIIAHRLSTIAHVDRIITLKGGRVDEIGTPNKLAKSGG
                     IYQQLLELQSTGLPKAKEELKHYGIADA"
     gene            2848..3327
                     /locus_tag="VFG56_02455"
     CDS             2848..3327
                     /locus_tag="VFG56_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6622773.1"
                     /translation="MELPMRKPRVTARVIVIDQGRLVLIKRRRYDFTRFKWREYYSIP
                     GGGVEPGESLEQAAKREFKEELGVDIAVDAPIAVGRSANYEHHLFTGRLLGGQPEWQT
                     DSEEMRFANQFNSYRLAWVAIEDLSQISLAYYAVFLPVILEVAAGRSPAEPVSYRLP"
     gene            3375..3932
                     /locus_tag="VFG56_02460"
     CDS             3375..3932
                     /locus_tag="VFG56_02460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6622774.1"
                     /translation="MATKTSQRVFIWVIAIVLTIGTIGTYAALVLGTSNQSSQAAEQQ
                     QLIEQYKQQIYDNNLPLDGYQAESFNAKSVDQLKVEDLKAGDGPVAKASDKLTVSYFG
                     WTPDGKIFDSSNKKDGTGQPYSFTPSEGGVIAGWVKGLPGMKAGGVRLLSIPADQAYG
                     STGSPPLIPANTPLKFIVRLDKIGS"
     gene            4019..4270
                     /locus_tag="VFG56_02465"
     CDS             4019..4270
                     /locus_tag="VFG56_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET6622775.1"
                     /translation="MSKDITIYTRTTCAYCVNVKKYLDMKQQAYTVINLDEQPQLADE
                     VIAKSGARTVPVVVVRDLATDTEDITVGWNPGKLAAALN"
     gene            4279..>4759
                     /locus_tag="VFG56_02470"
     CDS             4279..>4759
                     /locus_tag="VFG56_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6622776.1"
                     /translation="MADDKIHDVVMIGAGPSALTAAIYTTRENVDTVLIEKGVIGGLA
                     AITDWVDNYPGFSKGIEGLKLAEELEAQANRFGAKIEFGEVLGLEDRRDYKLVKTDSG
                     EVKAKAVLIATGSDYKKLGVPGEAEFYGRGVHYCATCDGAFYQDKTLVVVGGGNSAVQ
                     "
BASE COUNT         1230 a         1146 c         1210 g         1173 t
ORIGIN      
        1 caccagccgc gatcttacac tcgaagttgc cgataaggtt agttacgccg acgtcagcca
       61 agttataaac gaagccttaa ataacagcca gctgctgagt agtcttgagc tgacgcatat
      121 ctaccgagcc gatggtggaa ctactaagaa cctgactttc cgcgtccagg tgatcgatcc
      181 ggtcaagact atcacatccg atgaggctaa cctactaatc gagcaagtag cagaccaggt
      241 caagcacaag ctcggagcga aagtcgtcta aaaggaaagc ccccgatttc tgatcggggg
      301 cgtatgtcct ggttagtagt tgccgtggat tctgatgatc agatcttcga cgacttgttt
      361 aagcaactcg ggatctttca tgtcggacat gttatgaaag acctcatctt cgttagccgc
      421 cgtgtcgata gttaccgtcc gactattgcg gaagatgaac aattcggcta tggttcgctt
      481 ctccagctcg gccgtatcga ttttcctagt cggaatcatc ggcgacttat agttgagcca
      541 gaacagccgg ctggtcggga actcgatggt gataatggcg gctgtaatcc ttagtcgcca
      601 gtcacaccag tagatatagg atcgctgtag cgaccaccaa caaccgatca gtaccaaaat
      661 cagaaccagg ctagccgtca gctgtagata agccacgccg ctcggtgtaa aaatcagtat
      721 cacgatgaag acggcgatgg tagcgatggc gataccggct gaacgagcga tgctcttgag
      781 caggtatttg acgtgcttac caccgtagaa caaaccggct tgttcttcca gtaacccggc
      841 ggcctcatcg gttgcgtcaa gctcatcgtt aaccggcacc atttcttcgg tgggtggccc
      901 caagtcgtct gtagcactat tcatgatatc ctcccagagc ggttgaaaga cgaaggcttc
      961 gaaggtatat gtgcaaaccg tccccataag cgacaattca ttacttttat agactttttt
     1021 ggacctaaga gcaacgttaa gcgctatggg gagggccggg cttagtctat aatcggtaac
     1081 cgatgcgaat acttttcaag gtgttaaaat ctgctggcaa cctatggccg tattatttgg
     1141 ctattgcagc tttttcggtg gtcttgagtc tggcatcgct agcaataccc tttttgatta
     1201 aggctgccac tgacctgact gtgatgtcac tacagaccgg tcgggctgag gttagcggta
     1261 tcgtttggat tgccgtgatc atactgggcg tcgaagtagc tacgacgata ctgtccaact
     1321 ttgccggcta tttcggagac atgatggcgg ccaaactacg tcagcaattg tcggagcgtt
     1381 attatgccca cctccttaaa ctcccgcaga gttatttcga tcgggaactg acgggtacca
     1441 ttatcaatcg gctaaatcga accatcatgg aagtgacgca gtttttaaac gcttttgcca
     1501 acaacttctt ccagatgatt ctgatgataa ttctggtatt gatcatcgtc ggcttctata
     1561 gctggcaact ggctatttta ctattcgtgg tctacccgat attcctatac ctgaccatgt
     1621 tgaccagcag gaaatggcaa gtttatcaaa aacaaaagaa tctcgagacc gatgtcgctt
     1681 ccggtcgttt cgccgaagtc gtctctagta tacgagtggt caaaagcttc atccgtgaaa
     1741 gcttagaaaa gcgcgaattt tcgcatcact tttcgaccac ggtcgatatc actaaacggc
     1801 agtctagcta ctggcataag atggacgtct cgcgtcgttt ggtgctaaat ctaatcttct
     1861 ttatggtcta cgcttatatt tttgtggcta cggttcagag tcgtttcacc atcggcgaga
     1921 tggttctctt aatccaacta gttaatatgg tgcggatgcc gattttctcg atgagtttca
     1981 tcgtcgacaa tgctcaacga gcaattgccg gtagtgtcga ctacttcgag gtgatggcaa
     2041 ttgaaccgga tttggccgac aagccggctg cccaggcact caaggttact cgagcagcgg
     2101 ttacttacga tcgggttaac ttcgcctatc aatccggcca atcagttttg aagaatttag
     2161 acttctcgat taagcctggt gaaaaagttg ccctagtcgg cgaatcgggc gaggggaaga
     2221 cgacaatcac taatctgctg cttcgcctgt acgaccccaa caagggaagg atcgttatcg
     2281 atgatcagga tatagccaat gtcacacaag cttccttacg cgacaatata gcggtagtct
     2341 tccaggagcc agcattgttt tcaggcacca tcagagaaaa tatcgcctat ggtcagccag
     2401 gagctaccga taaggctatc cgagcggcgg ctaaggcggc taacgcgcta gagttcatcg
     2461 ataagtttga gcttggcttc gatacgacta taggcgagcg tggattgaag ctatctggcg
     2521 ggcaaaaaca acggattgcc atagcccgcg ccgccttaaa agacgcaccg attttggtac
     2581 tcgatgaagc cacctctagc ttggactcgc ggtctgagca tctagttcag caagcgttag
     2641 agcgattgat gaaacgacga actacattga ttattgctca cagattaagc acaattgctc
     2701 atgtcgaccg catcatcacg ctgaaaggcg ggcgggttga tgaaattggt acaccgaata
     2761 aactggccaa atccggcggc atctaccagc agctacttga gctacagtca actggtttgc
     2821 ctaaagccaa agaagagctc aaacactatg gaattgccga tgcgtaagcc gcgagttacg
     2881 gctcgagtta ttgtaatcga tcaagggcgg ttagtgctga tcaagcgtcg ccgttatgac
     2941 tttacccgct tcaaatggcg tgagtattat tccatacctg gtgggggtgt tgagccgggt
     3001 gagtcgctag aacaggcggc taaacgcgag ttcaaagagg agctaggggt agacatagcg
     3061 gttgatgcgc cgattgccgt aggacgatcg gctaattacg aacatcatct gtttacaggg
     3121 cgtctattag gcggtcaacc cgagtggcag actgattccg aagagatgcg cttcgccaac
     3181 cagttcaata gctatcggtt agcatgggtg gctatcgaag atctatccca aatcagcttg
     3241 gcctactacg cggtcttttt gccggtgatc ttagaagtgg cggccggacg atcaccggct
     3301 gagccagtca gctatcgact accgtaatgt agtacaatag atactaagtt ttaagaaaca
     3361 aagggaggtc agttttggct actaaaacca gccagcgagt atttatttgg gtcatcgcca
     3421 tcgtcttaac cattggtacc atcggtactt atgctgcctt ggttttaggt accagcaatc
     3481 agtccagcca agccgccgag cagcagcagt tgatcgaaca gtacaagcag caaatctacg
     3541 acaataacct gccactcgac ggataccaag ctgagtcgtt taacgctaag tcggttgatc
     3601 agcttaaagt cgaggatttg aaggccggtg acggtcctgt cgctaaagcc agcgacaagc
     3661 tgaccgtcag ctatttcggt tggacgccgg atggcaagat ttttgattcc agcaataaaa
     3721 aagacggcac cggtcaacct tatagcttca cgccgagtga gggtggcgtc atcgcaggct
     3781 gggtcaaagg cctgccaggc atgaaagccg gtggtgtgcg cctattgtct atcccggccg
     3841 atcaggctta tggctcaacc ggtagtccgc cgctaatacc tgccaatacg ccgcttaagt
     3901 tcatcgtccg tttagacaaa atcggttcat aatacaacat atagcgcatt gacggttatg
     3961 ttaaacgcta tatataatgt aatcaaaata atcaactaac agaaaaagaa gggttaagat
     4021 gagcaaagac atcaccatct acacgcgaac cacttgcgct tactgtgtca acgttaagaa
     4081 atacttagac atgaaacaac aggcttacac ggtcatcaat ctggatgaac aaccgcagtt
     4141 ggccgatgaa gttatcgcca aatccggtgc ccgtaccgta ccggtcgtag tggtgcgaga
     4201 tcttgccacc gacaccgaag atatcaccgt cggttggaac ccgggtaagc tagccgccgc
     4261 actgaattag gtcttataat ggcagacgat aaaatccacg atgtagttat gattggcgcc
     4321 ggtcctagcg ctctgacggc cgctatctac actacccgag aaaatgttga caccgttctt
     4381 atcgaaaagg gtgtcatcgg tggtctagcc gccatcactg actgggtcga taactatcct
     4441 ggtttttcca aaggtatcga aggcctgaaa ctagccgaag agcttgaagc ccaggcgaac
     4501 cgctttggtg ccaagataga gtttggcgag gtcctcggcc tggaagaccg tcgtgattat
     4561 aagctagtca aaaccgacag cggtgaagtc aaggccaaag ccgtactaat agccaccggc
     4621 tcggattaca agaagcttgg cgtaccgggc gaagctgaat tttacggtcg cggtgttcac
     4681 tattgtgcta cttgcgacgg tgcattttac caagataaaa cactggtggt ggtcggcggt
     4741 ggtaattcgg ctgtccagg
//
LOCUS       DASSBW010000087         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_547502, whole genome shotgun sequence.
ACCESSION   DASSBW010000087 DASSBW010000000
VERSION     DASSBW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547502"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFG56_02475"
     CDS             complement(<1..648)
                     /locus_tag="VFG56_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6622777.1"
                     /translation="MEGEGRSADYQGMSELPSVIAAAHELKSPLILMRQLSLELGETH
                     DPARAEVINQRLRFSLDQSLRLVEQLSLAPRLEDGIFGSEPVETQSVCTQLVNHLQPL
                     ANHLSVELDFNPKRQPLVALANRDLLPALLVNLCDNALSYTPAGGRVQITASRYQSGI
                     RLSVRDQGPSISRAAYRQLKSHLGKSAQPLGARPRSSGLGLWIAGRYAEAMSAKLS"
     gene            complement(675..1322)
                     /locus_tag="VFG56_02480"
     CDS             complement(675..1322)
                     /locus_tag="VFG56_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622778.1"
                     /translation="MNQRGSANPLLFSTIMFAVLTVALAGLFVWAFMNYQDYKHNTDQ
                     KVAAAVGAAKAEQKKQDDKQFEEDYKKPYTTYRGPADLGSVSFDYPKTWSAYEASNTA
                     DNNGLQAYFHPKVVPPIDTDGQVFALKVEVKDLTYQQSLDELSGNLEEGTLKAAPYKT
                     NGYTGMRFTGQLDESHQGVILMFKIRNQTLVITVNSNSFVKELDHTILKTLKFNP"
     gene            complement(1363..1857)
                     /locus_tag="VFG56_02485"
     CDS             complement(1363..1857)
                     /locus_tag="VFG56_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622779.1"
                     /translation="MLGGLFIALFILTFGVVFFGAPYVPTRQKDVEKLFDLVDLSDGR
                     LVDLGAGDGRLMLAAAKRQLNSTGWELSPVIWAIAWFRLRSHRRYADIRLGSFWRAKL
                     PDDTTAAFTFLASRHMKQLDGFLSREAKRLGHPIHLVSYGFQVPGRTIEKQDGALIVY
                     RFEP"
     gene            complement(1858..2061)
                     /gene="xseB"
                     /locus_tag="VFG56_02490"
     CDS             complement(1858..2061)
                     /gene="xseB"
                     /locus_tag="VFG56_02490"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6622780.1"
                     /translation="MPEKNNLKTDLKDLQAIVDWFEADDLDLDQAIAKFDEGVKKADD
                     IKKQLEQLENKITVLKQRFDEAA"
     gene            complement(2045..3163)
                     /gene="xseA"
                     /locus_tag="VFG56_02495"
     CDS             complement(2045..3163)
                     /gene="xseA"
                     /locus_tag="VFG56_02495"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6622781.1"
                     /translation="MENIPLQVSDAIALINQTLDYAYPTIVVEGEVSGFKINKDKYVF
                     FDIKDAEGSLNCFMMVFQLGIALEDGMKVRLVAKPNLTKWGKFSLTVRSVQPVGEGSL
                     KRAFELLKAKLDKEGLFAPERKRLLPSMPERVGVISSTGAAGYADFMKILNNRWQGVE
                     LLVANTQVQGEAAPTQMVRAIEHFNEMSQPVEALVIIRGGGSQDDLAAFNDERLVRAI
                     AASRTPTLVGVGHEVDVSLADLAADVRAATPSNAAEILVPDRRQLLEALAGSTSRMIS
                     SMHQTVRAYRRQTDEQLDRVLSRVDDTYLGAVKDYQNLTAVLRQLDPKLALKRGYALV
                     RDASGRLIGDKPPRSGQVLSVETSKFIIETGVKNAREK"
     gene            complement(3135..>3498)
                     /locus_tag="VFG56_02500"
     CDS             complement(3135..>3498)
                     /locus_tag="VFG56_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HET6622782.1"
                     /translation="RREPKILLEPYVWGIVFNIFEIAMFMITFWALGTWVNPAPLFIA
                     YGLAVTVGAVVATPGGTGGYEAIMILFLAAAGIPEGVVVAGVVLTRAILIVGTILSGY
                     VFYQLAIAKYGKHPTSSI"
BASE COUNT          856 a          960 c          841 g          841 t
ORIGIN      
        1 cgacaattta gcgctcatcg cttcagcgta gcgaccggct atccatagcc ctagcccact
       61 ggagcgtggc ctagcaccga gcggctgagc acttttgccc aagtggcttt tgagctgcct
      121 gtaagccgct cggctgattg acgggccttg gtctcgcaca ctcaggcgaa taccactctg
      181 atagcgagac gctgttatct ggacacgccc accagccggc gtataactaa gggcgttatc
      241 gcaaagattg accagcaagg ctggtagaag gtcccgattg gctagcgcca ctagcggctg
      301 gcgcttaggg ttaaagtcca gctcgaccga taagtgattg gctagcggct gcagatggtt
      361 gaccagctgc gtgcagacag actgcgtctc aaccggctcg ctaccaaaga taccgtcttc
      421 cagcctaggc gctaaactga gttgttctac caagcgcaga ctctgatcaa gactgaagcg
      481 caggcgctga ttgataacct cggctcgcgc cggatcatgt gtctcaccca gctccaagct
      541 aagctgacgc atcaaaataa gcggactttt caattcgtgg gcagccgcga tgacgctagg
      601 cagctcactc atgccctgat aatcagctga tctaccctca ccttccattg gctacaattg
      661 tagcactttt ggacttaagg attgaacttc aacgtcttca agatagtgtg gtctagctct
      721 ttgacgaaag aattggagtt aacggtgatt actagcgtct gattacgaat cttgaacatc
      781 aggatgacac cctggtgact ttcatcgagt tgaccagtga aacgcatacc ggtgtaacca
      841 ttggtcttat acggcgcagc cttcaaggta ccttcttcta gattgcccga taactcatcg
      901 agtgattgct gataggtcag gtccttgact tcaaccttca gagcgaacac ctgaccgtca
      961 gtatcgatcg gtggtacaac cttagggtgg aaatatgcct gtagaccgtt gttatcagca
     1021 gtattgctag cctcataggc actccaggtc tttgggtaat caaaactgac cgaaccgagg
     1081 tcggccgggc ctcggtaagt ggtgtacggt tttttgtaat cttcctcgaa ctgcttgtcg
     1141 tcttgcttct tctgctcagc cttagccgcc ccaacggcag cggcgacttt ttggtcggta
     1201 ttgtgcttgt agtcttggta gttcataaaa gcccagacaa acagaccggc cagagccacg
     1261 gtcaaaaccg cgaacattat agtcgaaaac aggagtgggt ttgcagagcc gcgttgattc
     1321 attaactata atcataacca gtcggattta tactgtaaag gcttatggtt caaagcggta
     1381 aacgatgagg gcgccgtctt gtttctctat agtccggccc ggcacctgaa agccatagct
     1441 aactaggtgt atcggatgac ctaaacgctt ggcttcccga cttaaaaaac cgtcgagctg
     1501 cttcatgtgt cggctggcga gaaaggtaaa tgctgccgta gtatcgtctg gtaacttagc
     1561 acgccagaag ctaccaagac gaatatcagc gtagcgcctg tggcttctca ggcggaacca
     1621 agcgattgcc catatcaccg gactaagctc ccatccggtt gagttcagtt gacgcttggc
     1681 tgcagccagc atcagtcgac cgtctccagc gccaaggtca accaggcgtc cgtcggataa
     1741 atcgaccaga tcaaacaatt tctcgacgtc tttttgacgg gtcggcacgt aaggtgctcc
     1801 aaagaagact acaccgaacg ttaggataaa taaggcgata aataagccgc cgagcactta
     1861 agccgcctcg tcgaagcgct gcttgagtac ggtgatctta ttttccagct gctcgagctg
     1921 cttcttgatg tcatcagctt ttttgacacc ttcgtcaaac ttagcgatgg cttgatcaag
     1981 atcaaggtca tcggcctcaa accaatcaac aatggcctgc aaatccttaa ggtcagtctt
     2041 caaattattt ttctcgggca tttttaactc ctgtttcaat tataaacttc gacgtttcaa
     2101 cgcttaatac ttggccggac cgaggcggct tgtcgccgat cagtcgacca ctagcatcgc
     2161 gcaccaaggc gtagccacgc ttcaaggcta acttggggtc aagctgcctc agaacggcgg
     2221 tgaggttttg gtagtcctta acggcgccaa gataggtatc gtccacccgg ctgagtactc
     2281 tgtctagttg ctcatccgtc tgccgacgat aagccctgac cgtctgatgc atagagctga
     2341 tcatgcgact agtactgcca gccaaagcct cgagtaactg ccgtcgatcg ggtactaaga
     2401 tctcggcggc attacttggc gtagcagctc tgacgtcggc cgctagatcg gctaacgata
     2461 cgtcaacctc atgaccgaca ccaaccagcg tcggcgttct ggaagcggca attgccctta
     2521 ccaatcgctc atcgttgaaa gcggctagat cgtcttggct cccaccgcca cgaatgataa
     2581 ccaacgcttc gaccggttgg ctcatctcat tgaagtgctc gatagcccga accatctgag
     2641 tcggtgccgc ctcaccctgg acctgcgtat tggcaaccaa tagctctacg ccctgccacc
     2701 gattgtttag tatcttcata aaatcagcgt aaccggccgc accggtacta ctaatcaccc
     2761 ctacccgctc cggcatggac ggcaaaagcc gcttgcgctc cggcgcaaac aacccctctt
     2821 tgtcgagctt agctttcagc aactcaaaag cccgcttcag actaccctca cccaccggct
     2881 gaaccgaccg aacagtcaag gaaaatttgc cccatttggt cagattgggc ttagccacta
     2941 gcctgacttt catgccgtct tctaaggcga tgcctaactg gaagaccatc atgaaacagt
     3001 tcagagagcc ttcagcgtct ttgatgtcga agaagacata cttatccttg ttaattttga
     3061 atcccgacac ttcgccttcc actacgatag tcggataagc gtagtcaagc gtctgattga
     3121 tcagcgctat agcgtcagat acttgaagtg ggatgttttc catatttggc tatcgctaat
     3181 tgataaaata cataaccgga taaaatagta ccgacgatca agatggctcg agtcaggact
     3241 acaccagcta ccactacacc ttccggtatg ccggcagcag ccaggaacag tatcatgatc
     3301 gcttcataac caccggtgcc gccaggagtt gccactactg ctccgacagt cacggccaag
     3361 ccgtaggcga taaacagcgg tgccggattg acccaggtgc caagcgccca aaaagtaatc
     3421 ataaacatgg ctatttcaaa gatattgaaa acgatgcccc agacatatgg ctctagtaat
     3481 atcttcggtt ctcgcctt
//
LOCUS       DASSBW010000088         5840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_724371, whole genome shotgun sequence.
ACCESSION   DASSBW010000088 DASSBW010000000
VERSION     DASSBW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724371"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..295
                     /locus_tag="VFG56_02505"
     tRNA            221..295
                     /locus_tag="VFG56_02505"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:254..256,aa:Glu,seq:ctc)
     gene            complement(436..828)
                     /locus_tag="VFG56_02510"
     CDS             complement(436..828)
                     /locus_tag="VFG56_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6622783.1"
                     /translation="MDKINSILIIEDDRFIGEMYVRSLKNAGYQVDWMVTGPDGLKAA
                     TDKVYDLILLDIMLPGAQGSDILSSLRGKEGDKVPNSRIIVLTNFDQDDETREAMLKK
                     VDAYLIKADITPRKLIDIIGTLKPARSA"
     gene            complement(835..2352)
                     /locus_tag="VFG56_02515"
     CDS             complement(835..2352)
                     /locus_tag="VFG56_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6622784.1"
                     /translation="MVVVRLADYYRNHRRSVIVATVGMQFLVTMSLVILLIGVGLSPL
                     IIAVYVVLPVVILQVFAFVVILNFTLAPLKVLSTVVTHVSKEPSDVKPPNLNGTYHER
                     TGLKRMVQTIYDLAVSGPTEVPTTNNPQFELTDSLMKDLPVGVIALDANHHIVYHNQA
                     APVHTDDQGNKSIELLFETSDPFERWLDEAENKSLMTQRAWTRISNALPDQPNRRVFD
                     VIANYQKERSSGLDTVIICVDRSEHYAVDEEDMDFIALAAHELRGPITVIRGYLDVLA
                     QETDLSTHPDHQVLLDRLDVSAARLSGYVGNILNVAKYDRRHLKLHLKEDRLSNIYQT
                     VADDLALRARAQGRSLSVNLPTDLPTIAADRNSLSEVMANLIDNAIKYSNEGGQVIVS
                     AAIDGDFVKFVVQDSGIGIPASVISNLFTKFYRSHRSATNVTGTGLGLYISKAIIESH
                     GGKIGVKSVEGEGSVFSFSVPIYATVKDKLLGGNNESAITMSAHGWIKNHSMYES"
     gene            complement(2346..3446)
                     /locus_tag="VFG56_02520"
     CDS             complement(2346..3446)
                     /locus_tag="VFG56_02520"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HET6622785.1"
                     /translation="MDDQDNYHLELRPNESKWHRQLRLGRGKKYFFLSLALIIAAGVG
                     LCGWMIWHPVNFAAKPVGYIVPPKPDPVYYSPLSGRKVSLADTKRPVTAVMIENSPWA
                     RPQSGLKQADVVFEAIAEGGITRFMALYQESQPTLLGPVRSLRPYYAEWGYAFDDSMA
                     HVGGSNAAKQMIRSGNYGVDLEQFFNGSYYWRSKDRYAPHNVYTSFKKLNALNKKKGH
                     TTSKFDGFPRKKDEPVEKPNAKTINIDISSSLYNVKYTYDAKDNSYWRYMGGEKHVDR
                     EKGQIKPKVVIVIKVREYTIPGGTHGRQDIQTTGSGKAYIFQDGTVTVGTWKKASAKS
                     QIHFVDKKGEDIALNRGQTWITAEPTDRSVKW"
     gene            complement(3484..4905)
                     /gene="gltX"
                     /locus_tag="VFG56_02525"
     CDS             complement(3484..4905)
                     /gene="gltX"
                     /locus_tag="VFG56_02525"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6622786.1"
                     /translation="MKVRTRFAPSPTGYLHVGGIRTALFAWLVARQTDGQFVLRLEDT
                     DKNREVTGSAEHLIKSLQTLGLDYDEGPDIGGTHGPYRQSDRLDIYKNWAQKLIDADR
                     AYADPYSSEEVQAFREQARRDKKAFLYRDHRPENPPAWDGTTPLRFKSEPKPYNWNDA
                     VMGDLKTGPEVVDDFILIKSDGFPTYNFAHIVDDAEMQITHVIRGQEFISSVPNYLNL
                     YEALDLKPPILATMPHIMNETGNKKLGKRDGAKDVLDYVRDGYLEEALVSFIATLGWN
                     DGSEQEVFTRDELIEKFSLERVQRSGARFDEKRLIWTNGQFIRRLSLDELYDRAEGFW
                     PTSAQSANATYKKQVLELVQDRIKTLSDLTTLIDYFFTEPQPDWALIDGNKQLKKLDR
                     QEIKQLLETTKDKLARIEWTSQNIQVALNQLLEETEQKPGILFSLIRIATTWAPFSPQ
                     LNDTLALLGQTKTLDRLERTIGS"
     gene            complement(4927..>5840)
                     /locus_tag="VFG56_02530"
     CDS             complement(4927..>5840)
                     /locus_tag="VFG56_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HET6622787.1"
                     /translation="DPVTPNVKPAVKKPSTGQSLSGIQKSVRGRQLVRKAQKSQTLKR
                     QHLKKPATHKTPVHQAKAPRAHQAVSRSHMISKFAPSAEPSKPVETVAENQPVTIVRP
                     HHVARATRPPITKPATKLSSSELKRQLIAKRLAEVDTKTRPSEVKVKQHRKRFSVMPI
                     VSGAFALVLLAGYLTYLNMPSLSVRVAAAQSNVAATYPGYQPDGYHLDGPVAYETGEV
                     ELNFKANQGTAAFTVAQRNSTWNSQAVYDNYVAKQGQSYQINTRQGITIYTYGNNAAW
                     VNGGIFYTISGSAPLSNDQLLHIATSM"
BASE COUNT         1292 a         1361 c         1595 g         1592 t
ORIGIN      
        1 gtagattcat aagcgttatt ataccactta tcaagcatag acaaactggc tgtaatttgc
       61 taaagttctt gaggtgcaac tccttatatg gttcgcatat agagccgtgt gctcggaata
      121 tgagaaaata tgagaactgg cctatattat gaatataggc ctcaaattat cggatcatag
      181 gaagttacgt aaacacaatt tccatacgtt cctcaaattt ggccctatcg tctagcggct
      241 aggacaccgc cctctcacgg cggcaacccg ggttcgattc ccggtagggt caccactaaa
      301 acagctcacc cgtaaaggtg ggctgtttta gtggtaatcc tgatagcaga accccacctc
      361 ctgggttgac taatcaaggc tacgtaccaa aggctgtacg aagtactttc ctttaggccg
      421 tggaagaacc gagccttaag ctgatcgagc cggcttaagt gtaccgatga tatcgataag
      481 cttgcgaggg gtgatatcgg ctttgatcag ataggcatcg actttcttga gcatagcttc
      541 cctggtctcg tcgtcttggt cgaagttggt taggacgatg atgcggctat ttggcacctt
      601 gtcaccttcc ttgccgcgca aactacttaa gatatcgctg ccttgggctc caggtagcat
      661 gatatcgagt aggattaagt cgtagacttt gtcggtggcg gcttttagac catcaggacc
      721 ggttaccatc cagtcgactt ggtagccagc gtttttgagt gatcttacgt acatctcgcc
      781 gatgaagcga tcgtcttcga taataaggat tgagtttatt ttgtccatat ggctctaact
      841 ttcgtacatt gaatggtttt ttatccagcc gtgagcactc atggtgatag ccgactcgtt
      901 attgccaccg agtaatttat ctttgacggt ggcgtaaatc ggtaccgaga agctgaagac
      961 actaccttca ccttcgaccg actttacgcc tattttacca ccgtgcgact cgataatggc
     1021 tttagaaatg taaagaccga ggccggtacc ggtgacattg gtggctgagc ggtgtgatcg
     1081 atagaacttg gtaaacagat tactgatgac tgaagctggg atgccgatac cgctgtcttg
     1141 gacgacgaat ttgacgaagt cgccgtcgat ggcggcgctg acgatgactt gaccgccttc
     1201 gttggagtac ttgatggcat tgtcgatcag attagccatg acttcgctca aagaattgcg
     1261 gtcagcagcg attgttggca aatcagtcgg tagattgacg cttaaagacc gcccctgggc
     1321 tcgggctctt agggctaagt cgtcggccac tgtctggtag atattggata gccggtcttc
     1381 tttaaggtgc agcttcaggt ggcgtcggtc gtacttggcg acattcaaga tattgccgac
     1441 gtagccggac aaacgggccg ccgaaacgtc gagccggtca agcaagactt ggtggtcggg
     1501 atgggtgctt aagtcggttt cctgagccag tacgtccaag taaccacgaa tgacagtgat
     1561 cggtccgcgc aactcgtgtg cggccaaggc gatgaagtcc atgtcttctt cgtcgacggc
     1621 gtagtgttcg gatcggtcga cgcagatgat tacggtatcg agaccagatg aacgctcttt
     1681 ttgataattg gcgataacat caaaaacccg gcgattaggc tgatccggta aggcgttgga
     1741 tattctggtc caggcacgct gcgtcatcag cgacttgttt tcggcctcat ccagccatct
     1801 ttcaaaggga tcgcttgttt caaacaataa ctcgattgat ttattgcctt ggtcgtcagt
     1861 atgaaccggt gccgcctggt tgtgataaac gatgtggtgg ttggcgtcga gtgcgatgac
     1921 gcctaccggc aaatccttca tgagactgtc ggttaattca aactgcgggt tattagttgt
     1981 cggtacttcg gttggtccgc ttactgccaa atcgtagatg gtctgaacca tccgtttcaa
     2041 gccggtgcgc tcatgatatg tgccgttcaa attgggcggc ttaacgtcag acggctcttt
     2101 cgagacatgg gtgactaccg tggacaggac tttgagtggt gcgagggtga agttcaaaat
     2161 gacgacgaag gcgaagactt gcaggataac taccggcaag acgacataaa cggcgatgat
     2221 gagcgggctt agtccgacgc caatgagaag gatgactaat gacatagtca ccaaaaactg
     2281 cataccgaca gtggcgacga tgacactgcg tcgatgattc cggtagtaat cggctagcct
     2341 aaccactacc atttgacaga cctatccgtc ggttcggcgg ttatccaggt ctgaccgcga
     2401 ttgagagcga tatcttcgcc ctttttgtct acgaagtgaa tctggctctt ggcgctggct
     2461 ttcttccagg tgccgaccgt gacagtaccg tcctggaaga tgtaggcctt gccgctgccg
     2521 gtagtttgga tgtcctggcg accatgggta ccgcccggta tggtgtattc gcgaactttg
     2581 atgactatga cgactttggg ttttatctgg cctttttcgc ggtccacatg cttctcgccg
     2641 cccatgtagc gccagtagct gttatccttg gcatcataag tgtatttgac gttgtatagg
     2701 ctggagctga tgtcgatatt gatagtcttg gcgttcggtt tttcaactgg ctcatctttt
     2761 ttgcgcggaa aaccgtcgaa cttcgatgtg gtgtgcccct tctttttatt gagggcgttt
     2821 aatttcttga agctggtgta gacattgtgt ggggcgtagc ggtccttgct tcgccagtag
     2881 taactgccat taaagaactg ctcaaggtcg acaccgtagt tgccggagcg aatcatttgt
     2941 ttggcggcgt tgcttccgcc gacatgagcc atcgagtcat cgaaagcgta accccactcg
     3001 gcgtagtatg gccttaagga cctgaccgga ccgagcaagg tcggttggct ctcctgatac
     3061 aaagccataa atctagtgat accgccctcg gctatggctt caaaaaccac atcggcttgt
     3121 ttgaggcccg attgggggcg agcccacggg ctgttttcga tcatcactgc cgtgaccggc
     3181 cgcttagtat cggccaggct gactttccga ccgctgagcg gtgaataata taccggatca
     3241 ggtttaggcg gcactatgta gccgaccggt ttggctgcga aattaaccgg atgccaaatc
     3301 atccaaccgc agagaccgac gccagcggcg atgatgagag cgagacttaa gaaaaaatac
     3361 tttttgcccc gccctagtct aagctgccga tgccattttg actcgttcgg tctcagctca
     3421 agatgatagt tatcttggtc gtccatttag ttaaattata gcaaaccgct tacgctaacg
     3481 ctgctaagaa cctatggttc gttctaaacg gtccaaggtt ttggtttggc caagtaaggc
     3541 caaagtgtcg tttaattgcg gagagaaagg cgcccacgtg gtggcaattc ggatgagact
     3601 aaataaaata ccgggttttt gttcggtttc ttccagtagt tggtttaagg ccacctggat
     3661 attttgactg gtccactcga tgcgggctaa tttgtcttta gtagtttcaa ggagctgctt
     3721 gatttcctgg cgatctaatt ttttaagctg tttattaccg tctattagcg cccagtccgg
     3781 ttggggttcg gtgaagaagt aatctatcaa agtagttaga tcgctaagcg tcttgattcg
     3841 gtcctggact agttctaaca cctgtttttt ataagtcgca ttggccgatt gggcgctggt
     3901 aggccaaaag ccctctgccc tatcgtatag ctcgtctagc gacagtcggc ggataaactg
     3961 gccattggtc cagattagac gcttctcatc gaaacgcgcc ccggagcgct ggacacgttc
     4021 tagtgagaat ttttcgatta actcgtcgcg cgtaaaaact tcctgctcgc tgccatcgtt
     4081 ccagccaagg gtggcgatga agctaaccag cgcctcctcc agatagccgt cgcggacata
     4141 gtctagtaca tccttggcgc cgtctctctt gccaagtttt ttgttaccgg tttcgttcat
     4201 gatgtgcggc atggtggcca agattggtgg ctttagatca agcgcctcat agagattgag
     4261 ataattcggc acactggata tgaattcctg gccgcggatg acgtgggtta tctgcatctc
     4321 tgcgtcgtca actatatggg cgaagttata ggtcggaaaa ccgtccgatt tgatcaagat
     4381 gaagtcgtcg acgacttcag ggccggtttt taagtcaccc atgacggcgt cgttccagtt
     4441 gtaaggctta ggttctgatt tgaatctaag cggcgtggta ccatcccagg ccggtggatt
     4501 ttccggtcgg tgatcgcgat acaagaaagc ctttttgtca cgccttgcct gttcacgaaa
     4561 agcctggact tcttctgagc tgtaggggtc ggcgtaggcc cggtcggcat cgatcagctt
     4621 ttgagcccag tttttgtaga tgtctaagcg atcggattga cgatatgggc catgtgtccc
     4681 gccgatatca gggccttcgt cgtagtccag ccctagtgtt tgcagagatt tgatcaggtg
     4741 ttcggccgaa ccggttacct cacggttctt gtcggtgtct tccaggcgca ggacgaattg
     4801 gccgtcggtt tgtcgagcca ctaaccaagc gaataaagcc gtccgtatgc cgccgacatg
     4861 taaatagcct gttggactag gcgcaaaccg agttcgtact ttcatgctag ataattatgc
     4921 cacagattac atgcttgtgg cgatgtgtag taactgatcg ttagatagag gggccgaacc
     4981 ggagatagtg tagaagatgc cgccatttac ccacgcggcg ttattgccgt aagtatatat
     5041 agtgatgcct tgacgggtat tgatctggta agactgaccc tgtttagcta cgtagttgtc
     5101 ataaacagct tgtgagttcc aagtactgtt gcgttgagcc accgtaaaag cggcggtgcc
     5161 ttggttggct ttgaagttaa gctcgacttc accggtctcg taggcaactg gcccgtccag
     5221 gtgataccca tcaggctggt agcccggata agtggcggcc acattggact gggcagcagc
     5281 tacacgtacc gacaaggatg gcatattgag gtaagttagg tagccggcca ataagactaa
     5341 ggcgaaggct ccgctaacaa tcggcataac tgagaatcgc ttccggtgtt gcttgacttt
     5401 tacttcagag gggcgagtct tggtatcgac ctcagccaag cgcttggcga taagttgtcg
     5461 tttgagctca ctcgacgata acttagtggc cggtttggtg attggtggac gggtggctcg
     5521 agcgacgtgg tgcggccgta caatggtgac tggctgattt tcggcgacgg tctcaactgg
     5581 cttagatggt tcagctgatg gagcaaattt actaatcata tgagaacgac taaccgcttg
     5641 atgagctcgg ggagctttgg cttggtgaac cggtgtttta tgggtcgctg gtttctttaa
     5701 gtgttgtcgt ttcagcgtct gactcttttg ggccttccta accagttgac gacctcggac
     5761 cgacttttgt ataccggaca ggctttgacc ggtcgaaggt ttcttgaccg ctggtttaac
     5821 gtttggagta accgggtctg
//
LOCUS       DASSBW010000089         3517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_459988, whole genome shotgun sequence.
ACCESSION   DASSBW010000089 DASSBW010000000
VERSION     DASSBW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459988"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /gene="rplX"
                     /locus_tag="VFG56_02535"
     CDS             complement(<1..230)
                     /gene="rplX"
                     /locus_tag="VFG56_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET6622788.1"
                     /translation="MVNKLQIKTGDNVMVTAGKHKGTAAKVVKTSPAKGLVFLENIGL
                     VKRHVKPTQLNPRGGSKEVHRGIDASNVKLVK"
     gene            complement(233..601)
                     /gene="rplN"
                     /locus_tag="VFG56_02540"
     CDS             complement(233..601)
                     /gene="rplN"
                     /locus_tag="VFG56_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET6622789.1"
                     /translation="MIQQESRLKVTDNSGAKEILCIRVLGGTKRRYARVGDLIVATVK
                     DANPGGTVKKKSVVKAVVVRTRDQIRRADGSTIRFDDNAAVIVGDDKQPRGTRVFGPV
                     PRELRDLGYIKIISAAPEVL"
     gene            complement(598..882)
                     /gene="rpsQ"
                     /locus_tag="VFG56_02545"
     CDS             complement(598..882)
                     /gene="rpsQ"
                     /locus_tag="VFG56_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET6622790.1"
                     /translation="MARKIIGTVVSDVQDKTIVVSLVSRRTHPLYGKQYTESRKFTAH
                     DEKNEAKVGDKVEIAESRPISKRKTWVLDHIVESGHAEVELKDETEEANL"
     gene            complement(882..1088)
                     /gene="rpmC"
                     /locus_tag="VFG56_02550"
     CDS             complement(882..1088)
                     /gene="rpmC"
                     /locus_tag="VFG56_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET6622791.1"
                     /translation="MAKQTTKSEQTPKQLHDQLAQLKSELLEAKQSLAAGELVNPRVI
                     TKTRKDIARIMTKLNQPTVKEEKD"
     gene            complement(1092..1505)
                     /gene="rplP"
                     /locus_tag="VFG56_02555"
     CDS             complement(1092..1505)
                     /gene="rplP"
                     /locus_tag="VFG56_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET6622792.1"
                     /translation="MLLPKKTKYRKVRIGTNKGVASRTNYLAFGDYGLQSLSNERITS
                     RQIESARQAMTRYIKRGGKIWIRIFPHTPVTRKPQDVKMGSGKGNPEFFVAKVKAGTV
                     MFEMRGVDEATAREAMRLAAHKLPVKTRFITRQEA"
     gene            complement(1505..2134)
                     /gene="rpsC"
                     /locus_tag="VFG56_02560"
     CDS             complement(1505..2134)
                     /gene="rpsC"
                     /locus_tag="VFG56_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET6622793.1"
                     /translation="MGQKVNPISMRLQVNKNWRSKWFATKKEYVNWLAEDLKIRSLVE
                     KRFTNRAVIDDILIERSANLLTVTIYTAKAGVVIGRGGAGVQELKADLEKVAGMPVRV
                     NIEEVKRPELAAKIVAENIARQLERRINFRRAVKMTAQNVMNAGAKGVRIEVAGRLNG
                     AEMSRREKLIRGSVPLHTLRADIDYHLAKAQTAAGIIGIKVWIYKGEAK"
     gene            complement(2134..2526)
                     /gene="rplV"
                     /locus_tag="VFG56_02565"
     CDS             complement(2134..2526)
                     /gene="rplV"
                     /locus_tag="VFG56_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET6622794.1"
                     /translation="MAETKTIKASLKGLRQTPRKVSLVASLVRGRSVADALVILEHTP
                     KRAAKPLAKLIASAEANAKTAHNLDGKSLVIDLLQVTAGPRLKRFRPGAMGRAKPYQK
                     KSSHVLVAVTGEVKLKKKPAAKATKEAK"
     gene            complement(2528..2791)
                     /gene="rpsS"
                     /locus_tag="VFG56_02570"
     CDS             complement(2528..2791)
                     /gene="rpsS"
                     /locus_tag="VFG56_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET6622795.1"
                     /translation="MSRSLKKGPFVDFKLAKKIAGLSNDDRTVIKTWARASVISPDMV
                     GRTIAVHNGRMHVPVYISENMVGHKLGEFAPTRKFRRHGGKEK"
     gene            complement(2792..>3517)
                     /gene="rplB"
                     /locus_tag="VFG56_02575"
     CDS             complement(2792..>3517)
                     /gene="rplB"
                     /locus_tag="VFG56_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET6622796.1"
                     /translation="SRAGRNNTGRITTRHRGGGVKQHYRLVNFRLAPGTTATVEHIEY
                     DPNRSARIARIKDQEGKYHYVLADTSMKPGKKITSGEQATVETGNRMPLEKVPAGTFV
                     YAVELQPGKGAQVIRSAGTKAQLMAKEGGYAMLKMPSGEVRRFRLDCMASIGVVGNVQ
                     HQNVKIGSAGRKRRMGIRPTVRGVVMNATDHPHGGGDGGRHGPGKAPRSPWGQLTLGY
                     RTRRRKDTAKAIVRSRHLAKRRK"
BASE COUNT          744 a          940 c          905 g          928 t
ORIGIN      
        1 tctttaacta acttaacatt agaggcatcg ataccacggt ggacttcttt gctaccgcca
       61 cgtgggttca gctgggtcgg cttaacatgt cgtttaacca aacctatgtt ctcaaggaat
      121 accaatccct tagctgggct ggttttgaca accttagcgg cagtgccttt atgtttgcca
      181 gcagttacca tgacattgtc accggtcttt atctgaagct tattgaccat agttagagta
      241 cctccggtgc ggcgctaata atcttgatat aaccgaggtc acgcagctcg cgtggcactg
      301 gaccgaagac acgagtaccg cgtggctgct tgtcgtcacc aacgataaca gcggcgttgt
      361 catcaaagcg gatggttgaa ccatcggccc gacggatctg gtcacgagtc cggacgacga
      421 cagccttgac gaccgacttc ttcttgactg taccaccagg gttagcgtct ttgaccgtag
      481 ctactatcag gtcaccgacc cgagcgtaac ggcgcttcgt gccaccaagt acccggatgc
      541 aaaggatttc cttggcgccg gagttgtcgg taacctttag cctagactct tgctgaatca
      601 tagattggcc tcctcggtct catctttcag ctcaacttcg gcgtggcctg attcgacgat
      661 atgatcgagt acccaggtct tgcgcttact gattggtcgc gattcagcga tctcgacctt
      721 gtcgccgact ttggcttcat tcttctcgtc gtgagcggta aacttgcgag actcagtgta
      781 ttgcttgccg taaagtgggt gagttcgacg gctaaccaaa cttacgacaa tggttttgtc
      841 ttggacgtca cttacgacgg tgccgataat ttttcttgcc atcagtcttt ctcctcttta
      901 acggttggtt gatttagctt ggtcataatt cgggcgatgt ctttgcgagt cttggtgata
      961 acacgcggat tgactaattc gccggcagcc aaggattgct tggcttcaag cagctcactt
     1021 tttagctggg ctagttgatc gtgtaactgc ttcggtgttt gttcggattt agtagtttgc
     1081 ttagccatga cttaagcctc ctgcctcgta atgaaacgag tcttaaccgg tagcttatga
     1141 gcagccaggc gcatggcttc gcgggcggta gcctcgtcga caccacgcat ctcaaacata
     1201 accgtgccgg ccttgacctt ggcgacgaag aactctgggt tacccttacc ggaacccatc
     1261 ttaacgtcct gaggcttacg ggttactgga gtgtgtggga aaatccgaat ccagatctta
     1321 ccaccacgtt tgatgtaacg agtcatcgct tgacgagccg attcgatctg cctggaagtg
     1381 atccgttcgt tagacaggct ctgcaaacca tagtcaccga aagctaagta gttagttcgg
     1441 ctagccacac ccttgttagt gccgattcgg actttgcggt atttggtctt ctttggtaac
     1501 agcattactt ggcctcccct ttgtagatcc aaaccttgat accgatgata ccggcagcag
     1561 tctgagcttt agctaggtgg tagtcaatat cggcccggag ggtgtgtaac ggcacgctgc
     1621 cacggattag cttctcacga cgcgacattt cagcgccgtt tagacgaccg gccacctcga
     1681 tgcgaacacc cttagcaccg gcattcataa cgttctgggc agtcatcttg acagcccgac
     1741 ggaaattgat ccgacgctcc aattggcggg cgatgttctc agctacgatc ttggcggcca
     1801 gttctggacg cttgacttct tcgatattga cacgaaccgg catgccggcg actttttcca
     1861 agtcggcttt aagctcttga actccggcac caccgcgtcc aatgaccaca ccggctttgg
     1921 cggtgtagat agtaacggtt agtaggttag ccgagcgttc gatcaagatg tcgtcgataa
     1981 cagcacggtt agtaaaccgc ttctcaacga gcgagcggat tttcaaatct tcggctagcc
     2041 agttaacgta ttctttcttg gtagcgaacc acttggaccg ccagttcttg ttaacctgaa
     2101 gccgcatgct aataggattt actttttgtc ccattattta gcctccttag tagcctttgc
     2161 ggctggtttc ttcttaagct taacttcacc ggttaccgct accaaaacat gcgatgactt
     2221 cttctggtat ggcttggctc ggcccatggc tccggggcgg aagcgcttga gtcgtggacc
     2281 agcagtaact tgcagtagat cgataaccaa acttttgccg tccaggttgt gggcagtttt
     2341 agcattggct tcagccgaag caatcagctt agccagcggt tttgcagctc gcttcggcgt
     2401 gtgttctagg ataactagag catcggcgac agagcgaccg cggactaggc tagccactag
     2461 gctaactttg cgaggagtct ggcgtaggcc tttgaggctt gccttgatag tcttagtctc
     2521 agccatacta cttctccttg cctccgtgac gacggaactt gcgagttggt gcaaattcac
     2581 ccaacttgtg gccgaccatg ttttcggaaa tgtataccgg tacgtgcatt cggccgttgt
     2641 gaacggcgat agtccgacca accatatccg ggctgatcac actggcgcgg gcccaagttt
     2701 tgattacagt ccgatcgtca ttcgacagcc cagcaatctt cttagccagc ttgaagtcga
     2761 cgaaaggacc tttctttaat gatcgactca tttattttct cctcttcgcc aagtggcgac
     2821 ttcttacgat tgctttagcg gtgtccttac gtcggcgggt gcgataacca agtgtcaact
     2881 gaccccaagg actacgcggt gccttaccag ggccgtggcg accaccgtcg ccaccaccat
     2941 gcgggtgatc ggtggcgttc atgacgacac cacggacggt cgggcggata cccattcggc
     3001 gcttacgacc ggctgaaccg atcttgacgt tctggtgctg gacattaccg actacaccga
     3061 tggaagccat gcagtccaaa cggaagcggc ggacttcgcc ggatggcatt ttcagcatgg
     3121 cgtaaccgcc ttctttagcc atcagctgag ctttagtacc agctgaacgg ataacctgag
     3181 cgcccttgcc aggttgcaat tcgacggcat aaacaaatgt accagccgga accttctcca
     3241 gcggcatacg attaccggtc tcaacagtag cttgctcgcc agaggtaatc ttcttgcctg
     3301 gcttcatcga ggtatcggct agaacatagt gatacttgcc ttcttggtct ttgatacgag
     3361 caatccgagc tgagcgattc gggtcgtact cgatgtgctc aaccgtagct gttgtgcccg
     3421 gagcgagccg gaagttgacc aaacgatagt gctgcttgac gccaccgccg cggtgacggg
     3481 tagtaatcct accggtattg ttgcggccgg cacgaga
//
LOCUS       DASSBW010000090         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1253938, whole genome shotgun sequence.
ACCESSION   DASSBW010000090 DASSBW010000000
VERSION     DASSBW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253938"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFG56_02580"
     CDS             complement(<1..335)
                     /locus_tag="VFG56_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6622797.1"
                     /translation="MKLYSWNVNGIRAVVKKGDFQKFIAEHQPDVLCLQETKAKQGQA
                     EVDLSGYEEYWNSADKAGYSGTAIFTKIKPLSVSFGFSDDIAERYNLAGDSYGDPAKE
                     GRMITAEFD"
     gene            433..852
                     /locus_tag="VFG56_02585"
     CDS             433..852
                     /locus_tag="VFG56_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622798.1"
                     /translation="MSTSVFDGKTTTTQSGLKFRLLDRPAWIEPPADELRSRILTHEQ
                     HLVLQGYVTEKTVSYTKGKLRHRMADVKKTTQRTIRLPAGSEVFFFSRDKKAPTTDQA
                     ICFVSNVWDEDCDREAQLTFYPKGLVRVRHYNPASFS"
     gene            complement(899..1363)
                     /gene="smpB"
                     /locus_tag="VFG56_02590"
     CDS             complement(899..1363)
                     /gene="smpB"
                     /locus_tag="VFG56_02590"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET6622799.1"
                     /translation="MAKAKPKKPAIVNRRAGYDYQLGDELVVGLELTGPEVRAARDGH
                     LQLKGSYVNISREELWLIGASFSLKLNEKGGANRTVDDRPRRLLARRREIENLAAQRK
                     QGLTIVPLRLLNRGRYIKLVIALGKGKKRYDKRQALKKRDVKRDINRVLKSR"
     gene            1480..2037
                     /locus_tag="VFG56_02595"
     CDS             1480..2037
                     /locus_tag="VFG56_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622800.1"
                     /translation="MLVANFRLAVNGWRDDPIKMTARLTASALIGQTIFWKFGYDQLG
                     VGMRPQKEFGQGRRVAAWAATKLTLDLQTAVALKVVEVLTDFGKGPLEAACYRRCGVV
                     PDALAFVVTQPLWSTFEAFEKSVSAVSDPRRLATPEQIELSLACIYGSLFRYGQAAIL
                     LLVGKRVNRLIVRPIEAAIRELLGK"
     gene            2095..>3629
                     /locus_tag="VFG56_02600"
     CDS             2095..>3629
                     /locus_tag="VFG56_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622801.1"
                     /translation="MNLTEDKDNLNAIAPGVVEAYAKLKSTDGMGIYTSAGRFYNHTL
                     FGRDASMSAKFVADFDHQVAVDTIMALAALQGTKTDSVTQEEPGRVHHELRDFTTWHG
                     AWPERLMLRFFKSQWGAQNDQLLTYFSVDSTASFVRLINKYAYHIDKSILSRKVTNHH
                     DEVVTVEQSLAEAADWLTRQIDTLGHFAPKRTNRRSLPFQIYEDSPTSYIRQDGSLLN
                     YKRPIAYASAQIFACDALEDASKLLPKHPRRHVWQDAAHQLRDSFIRDFWQEKQAYFA
                     SAIDSYGQSNLDSVSIGWTLNNGLWDELDKGLRAEKIGAIVRRLFSNGFLTKVGLRTR
                     AIDLPQPLNGTLEYHDADAVWPMFTFMVIEGLRRHKLYRLAEQLENRLLNGINAMGNF
                     PEFIDVDKDGRILRPVNRGEKHDFSVKAQMRPEECIAFTVVPSLTLARRIANGNQPKP
                     LTKGWQKELEDEILSGIDSVDLAEPTKAEDAIGTVVPIKLNRLAGNLRTVWFFLKESR
                     NLK"
BASE COUNT          869 a          946 c          911 g          903 t
ORIGIN      
        1 tcatcaaact ctgccgtaat catccgacct tctttggccg gatcgccata actgtctcca
       61 gccaagttgt agcgctcagc gatgtcatca gaaaagccaa aactgacact taaaggtttg
      121 atcttagtga aaatcgcagt accggaatag ccggctttgt cggcggagtt ccaatactct
      181 tcgtagcctg ataaatccac ttcagcttgg ccttgtttag ctttggtttc ttgcaggcaa
      241 aggacgtccg gctgatgttc ggctatgaat ttttggaagt cacctttttt gacgaccgct
      301 cgaatgccgt tgacgttcca agagtataat ttcatccctc tagtatacaa gataaataag
      361 ctgttgactg ccgcggcttc attcgctata aatagaccgt tccatcgttc tatcgccaag
      421 agaaaggcag tcatgtcaac gtcagttttt gacggcaaga cgaccaccac gcagagcgga
      481 ctcaagtttc gcctgctcga tcgaccggct tggattgagc ccccggccga tgaattgcgt
      541 tcgcgcatcc tgacgcatga gcaacacctg gtactacagg gctacgtgac cgagaagacg
      601 gtcagttata ccaagggaaa gctgcgccac cggatggctg atgtcaagaa gactacccag
      661 cgcacgatcc ggctaccagc cggaagcgaa gtcttcttct tcagtcgcga caagaaagcg
      721 ccgacgaccg accaggccat ctgcttcgtc tccaatgtct gggacgaaga ttgtgataga
      781 gaggctcagc tgaccttcta tccgaagggt ctggtgaggg ttcgccacta taacccagcc
      841 agcttcagct agtaagatcg atggaactcc cccgccagtt atggcggggg ttttcctttt
      901 agcgagattt caaaacgcga ttgatatcgc gtttgacgtc acgctttttc aaagcctgac
      961 gtttgtcgta gcgtttttta cccttaccaa gggcgatgac aagttttatg tatcggcctc
     1021 ggttcaaaag tcggagaggt acgatagtca gtccttgttt tctctgggca gctaaatttt
     1081 cgatctcccg tcgtctagct agtaacctgc gcggtcgatc gtcaaccgtc cgattagctc
     1141 cgcccttctc gttgagcttc aggctgaagc tggcaccaat tagccacagt tcctctcgcg
     1201 atatattgac ataactgccc tttagctgca ggtgaccatc gcgagcagcc cggacttccg
     1261 gtccggttag ttccagcccc acgactaact cgtcacctag ctgataatca tagccggcgc
     1321 gtcgattgac gatagctggt tttttgggtt tggctttggc cataatctat ctattgtaac
     1381 agaggccgct tggttgacaa aaactgattt aaatgcgtaa atattttaat caatctgggg
     1441 aaactcaggt tcaaagtacc tactatccaa ggagtcgcca tgctagtggc taattttcgc
     1501 ttagccgtta acggctggcg tgatgatccg atcaagatga cagctcggtt aactgcttcg
     1561 gcgcttatcg gccaaaccat tttttggaag ttcggctacg accagctcgg tgtcggcatg
     1621 cgtcctcaga aagaattcgg acaaggccga agagtggcgg cgtgggcggc gaccaaattg
     1681 acgctagact tgcagacggc agtggcactt aaggttgtcg aggttttaac tgactttggt
     1741 aaagggccgc tcgaagccgc ctgttatcgt cgctgcggtg ttgtgccgga tgcattagct
     1801 tttgtagtga cgcaaccgct ctggtcgact ttcgaagcct tcgaaaagtc ggttagtgcc
     1861 gtaagcgacc ctcgacgctt ggccaccccg gagcagattg agctctcgct tgcctgtata
     1921 tacggcagtc tattccgcta cggccaggcc gctatactgc ttctggtcgg caagcgggtc
     1981 aatcgactca tcgttcggcc gatcgaagcc gccatacgag aacttcttgg aaagtgaggt
     2041 aacaggccgc cctggtgaca gggcggctta tcaatttctg ctacacttat gtcaatgaat
     2101 ctgaccgaag ataaagataa tctaaacgcc atagcacctg gcgtagttga ggcttacgcc
     2161 aagctcaagt caaccgacgg catggggata tacacgagcg ccggccggtt ctataaccat
     2221 accttgtttg gtcgtgacgc cagtatgagt gctaagttcg tggccgactt cgatcatcag
     2281 gtagcagtcg atactatcat ggccctagcg gctctgcaag gtactaagac cgattcggtg
     2341 acccaagaag aacctggtcg agtccatcac gaattgcgtg attttacgac ttggcacggc
     2401 gcttggccgg agcgattgat gctacgcttc tttaaatccc aatggggtgc gcagaacgat
     2461 caattattaa cctacttttc ggtagactca accgccagct tcgtccgact gattaacaaa
     2521 tacgcctatc acatcgacaa gtctatcctg agccgtaaag tcaccaatca ccatgatgaa
     2581 gttgttactg tcgaacaatc actagccgaa gccgccgact ggttgactcg tcagattgat
     2641 acactcggcc actttgcacc taaacggact aatcgccgct cattaccctt tcagatctat
     2701 gaagattcgc ctaccagcta tatccgacaa gacggctctc ttttgaacta caagcggccg
     2761 atcgcttatg cttcggctca gattttcgcc tgcgacgccc tggaggacgc ctccaagttg
     2821 ttgcctaagc atccgcgtcg tcatgtctgg caagatgctg cccatcaact gcgagatagc
     2881 tttatccgtg acttctggca ggaaaaacaa gcttattttg ccagtgctat agactcgtac
     2941 ggacaaagca atctagatag cgtctcaatt ggttggacac tcaataatgg cctgtgggat
     3001 gaactggata aaggtttgag ggctgagaag attggtgcta tcgtccgacg gctgttctct
     3061 aacggtttct tgaccaaggt cgggctacgt acccgggcaa ttgacctgcc tcaaccgctc
     3121 aatggcactc tagagtatca cgacgccgat gctgtctggc cgatgttcac cttcatggtc
     3181 atagagggct taagacgcca caaactatat cgtctggcgg agcagctgga gaatcgcttg
     3241 ctcaacggca tcaacgccat gggtaatttc ccagagttta tcgatgtcga taaagatggc
     3301 cgaattttac gaccggttaa ccgcggcgag aagcacgact tctccgtcaa ggctcagatg
     3361 cgtccggaag aatgcatcgc cttcacggtg gtaccgagtt tgaccttggc tcgccggata
     3421 gctaatggca atcagcccaa gcccttaacc aagggttggc aaaaagaact agaagacgag
     3481 atattgtcag gtatcgactc agtcgattta gccgaaccga ctaaggccga agacgcgatc
     3541 ggtacagtgg taccgatcaa gctaaaccgc ttagccggca acctcagaac ggtttggttc
     3601 ttccttaaag agagtcgcaa tctcaaatg
//
LOCUS       DASSBW010000091         6334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_370438, whole genome shotgun sequence.
ACCESSION   DASSBW010000091 DASSBW010000000
VERSION     DASSBW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370438"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFG56_02605"
     CDS             complement(<1..375)
                     /locus_tag="VFG56_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET6622802.1"
                     /translation="MSKQLRDTEVVAVSFSVSFIDVIINLAVALLTGSAVMLAQMFQG
                     LSDLITAAFLYHGVRVSKRGRDERHDFGYGREVYFWVMVSAFFMFFGTGLLSFYFGYR
                     QFSQPEHVSNGWLALVVLGIVLV"
     gene            complement(692..766)
                     /locus_tag="VFG56_02610"
     tRNA            complement(692..766)
                     /locus_tag="VFG56_02610"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:Gln,seq:ttg)
     gene            complement(1013..1741)
                     /locus_tag="VFG56_02615"
     CDS             complement(1013..1741)
                     /locus_tag="VFG56_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HET6622803.1"
                     /translation="MFRRVVGESMSPVLQPGDIVVSLPLKVRAGDVVLATQAGREVVK
                     RVAKIDDRGYFLVGDNMTKSTDSRKHGSVKKQDILGRVIVRLPKAVAPVKPKTKLATY
                     LSLVLATMMVLLSVINLSRIDQLVPTLNGVLPGDQTTAAWAVIVIAMSQLLAVPYLLG
                     LKISPLAHVVSGFLSVLTPLIWCLIAIWSLGQVGSVGLFSSYIDLPASWPLTVLLLLW
                     LTASYVNLYYRNFEAQAQRLAKRA"
     gene            complement(1762..2145)
                     /gene="sodN"
                     /locus_tag="VFG56_02620"
     CDS             complement(1762..2145)
                     /gene="sodN"
                     /locus_tag="VFG56_02620"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HET6622804.1"
                     /translation="MKIFSIKPVYAHCDLPCGVYDPEQAMIEAKSVYAIQTKMKDFEG
                     DDRSRALIIKEQRAELVKHHLMVLWADYFKPEHLEKYPDLHDKFWKTIKQASECKHHA
                     EPEEGKKLLDMIKEISDIFAATRQG"
     gene            complement(2472..3149)
                     /gene="trmD"
                     /locus_tag="VFG56_02625"
     CDS             complement(2472..3149)
                     /gene="trmD"
                     /locus_tag="VFG56_02625"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET6622805.1"
                     /translation="MRRIQVVTLFPEMFDGVLNSSMMWKAQKDDHIEFRLINLRDFGL
                     GPRKQVDDTPYGGGDGMLLKPEPLFAAVEKAKETSPNAKVLIMTPRGQRWQQNLAQIY
                     ADSGDDFIFICGRYEGYDERITALVDQSISVGDYVLTGGELPAMTIIDSIVRLIPGVL
                     GGEASAEIESFSDGETLEFPQYTRPADFRGMKVPEVLLNGHHGEIAKWRQHNSHKAPT
                     TEDAEAK"
     gene            complement(3289..3729)
                     /locus_tag="VFG56_02630"
     CDS             complement(3289..3729)
                     /locus_tag="VFG56_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET6622806.1"
                     /translation="MLQLKILKQRRLTVSSIDQQFIEFIVKSLVEKPDAVKVERKIDE
                     RGVLLELTVDPEDLGRVIGKRGSTAQSLRSLLRALGTKNDARYNLKIVDVDRPNGEAS
                     TAKTSDGPSDNDVDNSQDEPVEKADSDRVKNTRKELAELDDLDI"
     gene            complement(3798..4178)
                     /gene="rpsP"
                     /locus_tag="VFG56_02635"
     CDS             complement(3798..4178)
                     /gene="rpsP"
                     /locus_tag="VFG56_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET6622807.1"
                     /translation="MLAIRLQRIGRKGHPVYRIAVQEAQRHPSSGRVVAYVGSYDPHS
                     KATKLNHDDLDKYLSNGAQPSPRVVRLLQSEKVKLPKWVQVPEQKSKSIKNVEKLRKN
                     RPAEEPAAAEASEETAPAEATSEA"
     gene            complement(4255..4986)
                     /gene="rnc"
                     /locus_tag="VFG56_02640"
     CDS             complement(4255..4986)
                     /gene="rnc"
                     /locus_tag="VFG56_02640"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET6622808.1"
                     /translation="MDLRSIDIKPYEDFTRDVLGFSFNNLELLVVAMTHRSYVNEHKK
                     STKEHNERLEFLGDAVLELVVTDFLYSNYQEPEGILTSWRSALVRTESISAAGDEIGY
                     EPLLRLSRGERRGSDRARAQILANAFEALTGAIYLDQGYETARDFIEKNILKKLPEIL
                     KEGSWRDPKSHLQEVAQSVDGQTPQYKVIEEVGPDHDKEFTLGVYVGNDLKGTGMGPS
                     KQVAQQAAAKAALKSYAERGLKSTD"
     gene            complement(4986..5477)
                     /locus_tag="VFG56_02645"
     CDS             complement(4986..5477)
                     /locus_tag="VFG56_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6622809.1"
                     /translation="MQKPKFDKFKKYFSKKQAIQEVVREPTAGGVIFRRNKKNKVEIL
                     LVQDAKNRWTIPKGHIEEGETAQQTAVREINEEVGLKEMEVICWLGKIHFRYRRVDTL
                     VLMTTQIYLVKALGDTNDIQKEDWMNGIKWMEFHDALEAIEYEDIGKLMLLAMKKIRQ
                     YKL"
     gene            complement(5464..5916)
                     /gene="nusB"
                     /locus_tag="VFG56_02650"
     CDS             complement(5464..5916)
                     /gene="nusB"
                     /locus_tag="VFG56_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6622810.1"
                     /translation="MASNRHLGRIIVLQSLYEYEFRLSCHDETVDAHAVLERNLERYK
                     DTVGDKEFIDKLYSGTIKHLESLDAIIQPLAPEWPIDQIAWVDRTILRMAVYELKHLA
                     KLVPPKVVINEAVELAKAFGADNSSKFVNGVLGSAYRSLYQDKQDAKA"
     gene            complement(5950..6105)
                     /locus_tag="VFG56_02655"
     CDS             complement(5950..6105)
                     /locus_tag="VFG56_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622811.1"
                     /translation="MAQPKKQSSPRKTGTRRSHLVLKLARKVNGRSPVKVRTTKRESG
                     RKKPATK"
BASE COUNT         1532 a         1624 c         1443 g         1735 t
ORIGIN      
        1 cactaggact atacccagaa ctactagagc tagccagcca ttgctgacgt gttcgggctg
       61 actgaactgg cggtagccga aataaaacga cagcaacccg gtaccaaaga acataaagaa
      121 agctgagacc atgacccaga agtagacttc acgaccgtag ccgaagtcat gccgctcatc
      181 gcgacccctc ttggaaaccc ggacaccgtg atataaaaaa gccgccgtga tgagatcgga
      241 caaaccctgg aacatttgag ccaacataac ggcactaccc gttagtaggg ctacggccaa
      301 gttgatgatc acatctatga agctgacgga aaatgaaacg gcgaccactt cggtgtcgcg
      361 aagctgttta ctcatatagt tagtccaatt atacagaaag agatcaggtt attcgatatc
      421 tttcttctag ttgagccaaa gggattcgct tcgctgggtc gaggacgacc gcgaagtgcc
      481 agggcttgcc gtaggggcaa gcccgttcga atcccatcca aggacaaatt aaaaaccgac
      541 caaacggtcg gattttaatc tggctggggt ggagggattc gcttcgctcg acaggagtct
      601 cgcgaagtgc cggggattgc cgtaggggca agcccgttcg aatcccgtcc aaggacaaat
      661 taaaaaccga ccaaacggtc ggattttaat ctggctgggg tggagggatt cgaacccccg
      721 aatgcatgga ccaaaaccat gtgccttacc acttggccac accccaataa ttgaagttga
      781 gcttatttgt aacgtacttt ggtggaacca gtttaggtcc atggactatt gcctatttac
      841 tcgcgcttcg cgctcagaca accatgtgcc ttaccacttg gccacacccc atcgtctaaa
      901 ttgcccctct tagctcgttt ttgctcttga gccaaactcg gctttgccga ctcaagcgat
      961 gagaagaacc ttcggtttgt ctcatttcgg caacaggggt aattataact atctaagccc
     1021 gctttgccaa gcgctgagct tgagcttcga aattgcggta atacaaatta acatagctag
     1081 ccgttagcca caatagcagc aatactgtca atggccaact agccggcaag tctatgtagc
     1141 ttgagaataa accaaccgat cctacctgac ccaaactcca tatcgcaatc aagcaccaga
     1201 taagtggcgt cagcaccgac aagaagccgc tgacaacatg ggctagcggg cttatcttca
     1261 gcccaagcaa gtacggtaca gctagaagtt gactcatggc gataactata accgcccacg
     1321 ccgccgtggt ctggtcgccg ggtagaacgc cgttgagtgt cggaactagt tgatcgatac
     1381 gcgataagtt gattaccgat aagagcacca tcatagtcgc caagactaga ctgaggtaag
     1441 ttgccagctt ggtcttaggc ttaaccggcg ctacagcctt gggtagccgg acgatgacgc
     1501 gccctaggat atcctgcttt ttgacactac cgtgttttct agaatctgtg cttttcgtca
     1561 tattgtctcc aactaaaaag taacctctat catctatttt tgcaactcgt ttgacgactt
     1621 cccgtccggc ttgagtcgcc agcaccacgt cgccagccct aaccttgagt ggtaagctca
     1681 cgacgatgtc accgggctgt aaaaccggtg acatactctc gccgactacc cttctaaaca
     1741 tctatcttcc gtaagttacg tctaaccttg cctggtcgca gcaaatatat cactgatctc
     1801 tttgatcatg tcgagcaact tcttgccttc ttctggctcg gcgtggtgct tacactcact
     1861 ggcttgcttg atagtcttcc agaacttatc gtgcaaatcc gggtactttt caaggtgttc
     1921 gggtttgaag taatcagccc acagtaccat caggtggtgt ttgaccagtt cggctcgctg
     1981 ttctttgata attagcgccc ggctacggtc atcgccttca aaatccttca tctttgtctg
     2041 gatagcatag acacttttgg cctcgatcat agcttgctcc gggtcataga ctccacacgg
     2101 caaatcgcag tgagcataaa ctggcttgat agaaaatatt ttcataaacc ctccttgttt
     2161 cacctacatt ataagccagc ttgcgcgact atcataaata tagcgttgta tttttacata
     2221 ttacgcttgt gtttctcctt tagtatgagg taaagtgtgt ttaagcgatt caccaaaaca
     2281 aaaacaccaa aacaccgtgg ggtttcgcct attctccacc acaacacctt aagcccagcg
     2341 acagaaagtc ctgggcttta acggtttcaa aatccaggca cctgcaccac ttacccttaa
     2401 tcctccagat acattatttt taacccgacc aggttaaaaa taatgtatct agatttattt
     2461 gttcagtttg attatttggc ttcagcgtct tctgtggtcg gtgctttgtg tgaattgtgc
     2521 tggcgccact tggcgatctc tccatgatga ccgttcagta gtacttcggg taccttcatg
     2581 cctcgaaaat cggctggtcg ggtgtactgt ggaaactcca gcgtttcacc gtcagaaaaa
     2641 ctttcaatct cggccgacgc ttcgccgcca agtactcccg gaatcagccg gactatcgaa
     2701 tcgatgatgg tcatagccgg cagctcgcca ccggtcagga cgtagtcacc cactgatata
     2761 gattgatcca ctagcgcagt aatccgctca tcgtagccct cgtagcggcc acatatgaag
     2821 ataaagtcat cgcctgaatc ggcatagatt tgggctagat tctgttgcca gcgctgacca
     2881 cgcggtgtca ttattaggac tttggcattt ggcgaagttt ctttagcctt ctcaaccgcc
     2941 gcaaataacg gttcaggttt gagcagcatg ccgtcgcccc cgccatacgg tgtgtcgtcg
     3001 acttgtttac gtgggcccaa gccaaaatca cgcaagttta tcagcctaaa ctcgatatga
     3061 tcatctttct gtgccttcca catcatgcta gagtttaaga ctccgtcaaa catctccgga
     3121 aacagcgtga caacttggat tcttctcatc tgttatattt taccaccaaa aaaccgcctt
     3181 gattttgagg cggtttttta gcacatataa gactcacaat cagctgatac agccgatgct
     3241 cgcatagagc ttatagttct cgcagtaatt gtttgaactt atgtttaatt atatatcgag
     3301 gtcatcgagc tcggcaagct ctttgcgagt atttttgact cggtcagaat cggccttttc
     3361 cacaggctca tcttgagaat tatccacatc attgtcagat ggcccgtctg atgttttggc
     3421 agtgctggct tcaccgttcg gtcgatcgac atcaacaatc ttaagattgt agcgagcgtc
     3481 gttcttggtc cctagggcac gtaagagtga gcgcaagctc tgggcggttg aaccacgctt
     3541 accgataacc cgtccaaggt cttctgggtc aacggtgagc tccagtagga cgccccgttc
     3601 gtcgatcttg cgttcgacct tgacggcgtc gggcttctcc actagggatt tgacgataaa
     3661 ttcgatgaat tgctggtcga tactagacac tgtcagcctc ctttgcttaa gtatcttcaa
     3721 ttgtagcatt tatgggactt tttgggcagc caaaaccccg ccggatcagg cggggttttt
     3781 ggatttaaca gtcttattta ggcttcgcta gtcgcttcag caggcgcagt ctcttcactg
     3841 gcttcagctg ctgcaggctc ttcagctggt cggttcttgc gtagcttctc tacgttcttg
     3901 atcgacttgc tcttttgctc tggcacctgg acccacttag gtagtttaac cttttctgat
     3961 tgcaataacc gaactacacg cgggcttggc tgagcgccgt tagacaggta tttgtccaag
     4021 tcatcgtgat ttagtttagt tgctttgctg tgtggatcat agctaccgac ataagcaacg
     4081 acgcgaccgc tagatgggtg gcgttgagct tcttgcacgg cgatgcgata aaccgggtga
     4141 cctttacggc cgattctttg cagacgaatt gccagcatta atttctaact tcccttttct
     4201 ttaatacttg atataaacaa tcttggacga ttatagcgac tcaagagcct cttgtcaatc
     4261 tgttgacttg agtccacgct cggcgtagct tttgagtgcc gccttggcgg ccgcttgttg
     4321 ggcgacctgc ttacttggac ccataccggt gcctttgaga tcgttgccaa cataaactcc
     4381 aagagtaaac tctttgtcat gatccggacc cacctcttcg atgactttgt actggggagt
     4441 ttgaccgtca acgctctgtg ccacttcttg caggtgtgac tttgggtctc gccaagagcc
     4501 ttcctttagg atttcaggta gtttcttgag tatattcttc tcgataaagt ctctagcagt
     4561 ttcgtagccc tgatccaagt agatggcgcc ggtgagggct tcaaaggcat tggccaatat
     4621 ctgggcccta gctcgatcag aaccccgtcg ctcgcctcgc gacaagcgta gcaatggttc
     4681 gtagcctatc tcgtcaccag ccgccgagat actctctgtc ctaaccaggg ctgagcgcca
     4741 cgacgtcaag ataccttctg gttcttgata attgctatac aaaaaatcag ttacgactag
     4801 ttccagtacg gcatcaccta agaattccag tcgttcattg tgctctttag tcgacttctt
     4861 gtgctcgttg acgtagctac gatgggtcat ggcgacaacc agcagctcta gattattaaa
     4921 actaaacccc aaaacgtctc gggtgaaatc ctcatagggt tttatgtcga tgcttcttaa
     4981 gtccattaca gcttgtattg ccttatcttt ttcatcgcca gaagcatcag cttaccgata
     5041 tcttcatact cgatggcttc caatgcgtcg tggaactcca tccacttgat accgttcatc
     5101 cagtcttctt tctgtatatc attggtgtca ccgagagcct tgaccagata gatctgggtg
     5161 gtcataagga ctagagtgtc aacacggcgg tagcggaagt ggatcttacc aagccagcag
     5221 atgacttcca tctctttaag gccgacctct tcattgatct cacgaacggc agtctgctgg
     5281 gcggtctcac cctcttcgat atgacccttc gggatagtcc agcgattctt ggcgtcttgc
     5341 actaacagaa tctctacttt gtttttctta ttgcgacgaa aaataacacc accagcggtt
     5401 ggctcgcgga caacctcttg gatagcttgc ttcttggaaa agtacttttt aaacttatcg
     5461 aacttaggct tttgcatctt gtttatcctg atacagagaa cgataagcgc tgcctaaaac
     5521 gccgttaaca aacttggacg aattatcggc cccgaaagcc ttagccaatt caacggcttc
     5581 gttgatgacg accttgggtg gtacgagttt agctagatgc ttaagttcgt agaccgccat
     5641 acgcaagatg gttcgatcga cccatgcgat ctgatcgatc ggccactccg gggctaatgg
     5701 ctgaatgata gcgtcaagcg actcaaggtg cttgatagta ccgctataaa gtttatcgat
     5761 aaattcctta tcgccgaccg tatctttgta gcgttcaaga ttgcgctcta gcaccgcatg
     5821 agcgtcgacc gtctcgtcat gacagcttaa gcgaaactca tactcgtaca gactctgcag
     5881 aacgatgata cgaccgaggt gacgattgga ggccataagg gataatccgt ttaatttgtt
     5941 aagttaaagt tacttggtag ctggcttttt gcggccagac tcgcgtttag tagtccgaac
     6001 tttgaccggt gagcggccgt taactttccg ggctaactta agtaccaaat gacttcgcct
     6061 agtgccagtc ttacgtggac tggattgctt cttgggctgc gccaattcat tactccttat
     6121 ttaatatttg tagacttgtc ttaaatatta gcaaaaacag ggataaaact caaggtcaaa
     6181 ccagttgtat taggcaaaat cccaagcctt tatttgacaa ctagattgac gatgcgacca
     6241 ggcacgtaga tcaacctaga cggttgctta gcgccaagcg cacccttcac cttctcgtca
     6301 gccaaagcag ccgcttcaac ggaagcttgg tcgc
//
LOCUS       DASSBW010000092         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1696651, whole genome shotgun sequence.
ACCESSION   DASSBW010000092 DASSBW010000000
VERSION     DASSBW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696651"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFG56_02660"
     CDS             complement(<1..405)
                     /locus_tag="VFG56_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622812.1"
                     /translation="MAKPNFQKRLVHNAPYILLGVVVLFVLGALVAGEILGAYDKFWW
                     WDDGLHTLAGAILGFIGFVLVYFFNARYQMHINPLFVAVFAFSFAVTMGVVWEIFEFT
                     GDALFRTNMQRWADPATAKMIGQTYQGSGLRDS"
     gene            complement(407..724)
                     /locus_tag="VFG56_02665"
     CDS             complement(407..724)
                     /locus_tag="VFG56_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HET6622813.1"
                     /translation="MSNTDLTLKDLQKHLEATCRERGWDQRSITECFLLFSEEIGELA
                     KAIRKETGIKGEARPEDSRDNLRGEFADVLNYLLELANRFDVDLTEAYFEKNAINQKR
                     TWN"
     gene            complement(735..1145)
                     /locus_tag="VFG56_02670"
     CDS             complement(735..1145)
                     /locus_tag="VFG56_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6622814.1"
                     /translation="MSKRIFVNLPVDDLERSTAFYEALGFGKNAVFSDERASCMVWSD
                     EIYVMLLTKDFYKHFTDGKDIIDAKKTSGTLLAISLDSKDEVQRFADTAKEAGGRVYQ
                     VDMGGGDMMFGYEVEDLDGHIWEPHWMDEAAMQS"
     gene            complement(1260..1754)
                     /locus_tag="VFG56_02675"
     CDS             complement(1260..1754)
                     /locus_tag="VFG56_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622815.1"
                     /translation="MSEQLEPPSGEINRQQKQAFIDRVTSLAPYTGQRIDSFGCYCTN
                     FETDDGYISIYIPVIAELASEDEPIDDAVRVVVRSEEALDTGQTIVTNRSYTIHVTDY
                     ETSYAKRISVFDSETGEPVRHEQEVTVEESLEIHDLNKSLNSSTFTAKRLKEVESKLE
                     RLEL"
     gene            complement(1764..2108)
                     /locus_tag="VFG56_02680"
     CDS             complement(1764..2108)
                     /locus_tag="VFG56_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622816.1"
                     /translation="MNERKPSHLTPEQAADVIRYYDRDANGEARARGWRDKVALGEVA
                     ARTTLKRFSILPNQTKPMLTPEEMGAGLDYIQNPPEPDKSEAYYQKLDELADKLGNYD
                     LARRQLDADGWQ"
     gene            complement(2184..>3113)
                     /locus_tag="VFG56_02685"
     CDS             complement(2184..>3113)
                     /locus_tag="VFG56_02685"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET6622817.1"
                     /translation="EPRAFNMMFKTNVGPVADDKSVSYLRPETAQGIFTNYKNVVDSF
                     YPDLPFGIAQQGKAFRNEISPRDFVFRSREFEQMEIEYFVEPTKWEEAFAMWQKLGHD
                     WCDALGLPKDMVHELEVPEDDRAHYSKRTIDFEFDFPIGREELFGLAYRTDFDLGNIQ
                     RASGKSMEYTIKGTNDKFIPHVIEPSFGLERAIMAVMTTAYTEDEVNGEKRIFMKFPE
                     HLAPVKYAVSPLLKNKPELVGKAKQVYDALKKKHGAVMWDDNGNIGKRYRRQDEIGTP
                     HCVVIDFDTLEDDTVTIRDRDTTEQRRVKIGEL"
BASE COUNT          699 a          830 c          744 g          840 t
ORIGIN      
        1 cgaatccctt aggccgctac cctgataagt ctgtccgatc atcttggccg tagctggatc
       61 ggcccagcgc tgcatgttag tacgaaatag cgcatcgcca gtaaactcaa aaatctccca
      121 gactaccccc atggttacgg cgaagctaaa agcaaatacc gccacaaaca aagggttgat
      181 atgcatctga tagcgagcat tgaagaaata gactaggaca aaaccgataa atcccagtat
      241 cgcgccggcc aaagtgtgca ggccgtcatc ccaccaccag aacttgtcat aagcgccaag
      301 tatctcaccg gccaccaaag caccgagaac gaaaaggact acgacaccga gcagaatgta
      361 cggtgcgtta tgaaccagtc tcttttggaa attcggctta gccacgtcag ttccaggttc
      421 gtttttggtt gatggcgttt ttttcgaaat aggcttcggt cagatcgacg tcgaagcggt
      481 tggctagctc caatagatag ttgagcacgt cggcaaactc accacgtaaa ttgtcccgac
      541 tatcttccgg tcgagcctcg cccttgatgc ctgtctcttt gcggatggct ttggccagct
      601 cgccgatctc ttccgaaaac aacaaaaagc attcggtgat actgcgctgg tcccagccac
      661 gctcacgaca agttgcctct aggtgttttt gtagatcttt cagtgttaag tcagtgttgc
      721 tcatctagta cactttaact ctgcatggcc gcttcgtcca tccaatgcgg ctcccagatg
      781 tggccgtcaa ggtcctcgac ttcgtagcca aacatcatat ctccgccacc catatcgact
      841 tggtagaccc gaccgccggc ttctttggcg gtgtcggcaa agcgctgtac ttcgtcttta
      901 ctgtccagac tgatcgccag caaggtaccg ctggtcttct tggcgtcgat gatgtctttg
      961 ccatcggtga aatgtttata aaaatctttg gttagcagca tgacgtagat ctcgtccgac
     1021 cagaccatac atgaggcccg ttcgtctgaa aaaaccgcgt ttttgccaaa tcctagcgcc
     1081 tcataaaacg ccgttgatcg ctccaaatca tccactggta agttcacaaa aattcgctta
     1141 ctcatagcat cctccttgaa gatttaaaaa gacttacaca actaattata taagtctttt
     1201 tgcctaggag tttcagtgaa gtatttctac tctctgccaa gttattggca ctggactagc
     1261 tataactcaa gccgctctag tttactctcg acttctttta accgcttagc cgtgaaagtc
     1321 gagctattca gcgacttatt gagatcgtgg atctcgagcg actcttctac tgtgacttcc
     1381 tgctcatgcc gaacaggttc gccggtctcg ctgtcaaaca cgctaatcct cttggcataa
     1441 ctcgtctcgt aatccgtcac atgtatcgta tagcttcggt ttgtaacaat agtctggccg
     1501 gtatcaagcg cctcctcgct acggacgact acgcgaaccg catcatcgat gggctcgtct
     1561 tcgcttgcga gttcggctat aacagggata tagatgctga tatagccatc gtcagtctca
     1621 aaattagtac agtagcagcc aaagctatcg attcgttgtc cagtatatgg cgctagacta
     1681 gtcacacgat ctataaaagc ttgtttttgt tgtcggttta tttctccgct tggcggctct
     1741 aactgttcac tcatagcgat agcctactgc cagccatcgg cgtcgagttg tcgtcgggct
     1801 aagtcatagt tacctagttt gtcggctagt tcgtccagtt tttggtagta ggcctctgac
     1861 ttgtctggct ctggcggatt ttggatgtaa tcaagcccag cgcccatctc ttccggtgtc
     1921 agcatcggct tggtctggtt cggcaatata ctgaagcgct tgagagtcgt ccgtgcggca
     1981 acttcaccta atgcaacctt gtcgcgccac cccctagccc gagcttcgcc attggcgtct
     2041 cgatcataat aacgaatcac gtcagccgct tgctccggag ttagatgact aggttttcgt
     2101 tcattcatag tactattata gcataaacac tataaattgt ctatgcatgc cagatcaggt
     2161 gaagattatt ggtactacta ttgttagagt tcgccaattt ttacccgtct ttgctccgtc
     2221 gtgtcgcggt cgcggatagt gacggtgtcg tcttccaggg tgtcgaagtc gatgacgacg
     2281 cagtgtggcg tgccgatttc gtcctgacga cggtagcgct taccgatgtt gccgttgtcg
     2341 tcccacatga cggcgccgtg cttcttcttc agcgcgtcgt agacttgctt ggcctttcct
     2401 accagctcgg gcttgttctt aagtagcggc gagacggcgt atttgaccgg ggctaggtgt
     2461 tctgggaact tcataaagat ccgcttctca ccgttgacct catcctcggt ataggcggtg
     2521 gtcatgaccg ccatgatagc ccgctccaaa ccaaaagaag gttcgatgac gtgtgggatg
     2581 aatttgtcat tggtaccttt gatggtgtat tccatactct taccactggc ccgctggatg
     2641 ttaccaaggt cgaagtctgt ccgataagcc aggccgaaca gctcttcacg gccgatcgga
     2701 aaatcaaact caaagtcgat ggtgcgcttg gagtagtgtg cccggtcgtc ttccggcact
     2761 tccagctcgt ggaccatgtc tttcggcaga cccagagcgt cacaccagtc gtgaccaagt
     2821 ttttgccaca tggcgaaggc ttcttcccac ttagtcggct cgacaaagta ctcgatctcc
     2881 atctgttcga attcgcgcga gcggaagacg aagtcgcgtg ggctgatctc gttacgaaaa
     2941 gctttgccct gctgggcgat accgaacggc aaatccggat agaaactgtc gactacgttc
     3001 ttgtagttgg tgaaaatgcc ctgggcggtt tccggtcgca agtagctgac cgacttgtca
     3061 tcggctaccg ggccgacgtt ggtcttaaac atcatattga aagccctagg ctc
//
LOCUS       DASSBW010000093         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_546579, whole genome shotgun sequence.
ACCESSION   DASSBW010000093 DASSBW010000000
VERSION     DASSBW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546579"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFG56_02690"
     CDS             <1..204
                     /locus_tag="VFG56_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622818.1"
                     /translation="HDGAVDAEKLYQDMLLVLGNISRYEMLCGVHNPTSDTWEARAAR
                     VLHYRGHEFVQHLPTRFRHPDND"
     gene            254..823
                     /locus_tag="VFG56_02695"
     CDS             254..823
                     /locus_tag="VFG56_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET6622819.1"
                     /translation="MAFEDYQTKDSVVIVYTGNGKGKTSASVGLMARALGRGFTVAFV
                     QFIKTWPTGEHQFIEQVMPSFKNQLTFYKGGKGFYNAGELSEAKVSETEHREAARSTF
                     DFALDCAKSDDYDIVICDEISNAVHDGLLSAKDLNKLLESRAKKTSLCLTGRNFPEEL
                     LGKADIVTSMTKVKHHFDDKFLANVGIDY"
     gene            complement(862..>2087)
                     /locus_tag="VFG56_02700"
     CDS             complement(862..>2087)
                     /locus_tag="VFG56_02700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6622820.1"
                     /translation="VKRELEAAGHEVYVFCPARSIRRTKHARVFKEDDRIIRFPSVKG
                     AFYEDYDTSLFFPPRVLRQIRELDLDVIHFFDPGQVGLMAVYAAYKTDTPLIAQHSTD
                     LREYVEHYRDTLLLPGLLTLIAFLPVVVKLNGRDLKEIMKLYRPRRGSVAWNIDIVER
                     MVTLIYSKCDAVIALSKKSQKQLESWQYADHYTYQVSLLPNGVNAIKKPTPKQVEDFR
                     SAWQIEPGDEVIGFVGRLGSEKNLVLLLKAFEKVIKNRPKAKLLFVGDFEFREVLEEL
                     ANKSKASDRIIFTGALPRETLGRVYSSMNLFVFPSLTDTQGWAIHEAALSGLPLILVD
                     KDLSEVMQDGVNGYYAKNQVSDLADKITDILKSPKKQADFGEASQKLARRFTEKRQVA
                     KLIKLYETAIDQHYQ"
BASE COUNT          533 a          508 c          520 g          526 t
ORIGIN      
        1 cacgacggcg ccgtcgatgc cgagaagtta taccaagaca tgctgttagt acttggcaat
       61 atatcccgct acgaaatgct gtgcggtgtc cacaatccaa ctagcgacac atgggaagct
      121 cgagctgctc gcgtcttaca ttaccgcggt cacgagtttg ttcagcacct accgactcgc
      181 ttccgacatc ccgataacga ttaggggagc tgcagagact caagttgtct agaaattagc
      241 gtatagtaaa gctatggctt ttgaggatta tcagactaaa gattcggttg tcatcgtcta
      301 cacaggcaac ggcaaaggta agactagtgc tagtgttgga ttgatggcga gagccttagg
      361 ccgtggcttc acggtggctt ttgtccaatt tatcaagaca tggccgaccg gcgaacacca
      421 gtttatcgag caggttatgc caagctttaa aaaccaattg actttttata aaggtggcaa
      481 aggcttttat aacgccggtg aactgtcgga agccaaagtt agcgaaaccg agcaccgaga
      541 ggccgctcga tccaccttcg acttcgcctt agactgcgcc aaatccgacg actacgacat
      601 agttatctgc gacgaaatca gtaatgctgt tcatgatggt ttactcagcg ctaaggactt
      661 aaacaagtta ttggagtcac gcgctaagaa aaccagcctc tgcttaaccg gacgtaattt
      721 tccggaggaa ctactaggta aagccgatat agttaccagt atgaccaaag tcaaacatca
      781 cttcgatgat aaattcttag ccaatgttgg tatcgactac tagcccacgg ctgtgttgtt
      841 cggggaaaac agccggcgat actactggta gtgttgatcg atagctgttt cgtagagctt
      901 gatcagctta gctacttggc gtttttcggt gaaacgacgg gctaatttct ggctagcctc
      961 cccgaagtcg gcctgcttct tgggactctt caagatatcg gtgattttgt cggccagatc
     1021 gcttacttga ttcttggcgt agtagccatt tacaccatcc tgcataactt ctgacagatc
     1081 tttatcaacc agtatgagcg gcagaccaga taaggccgcc tcatgaattg cccagccttg
     1141 cgtgtcggtc aaggaaggga agacgaatag attcatgctt gagtaaaccc ggcccagtgt
     1201 ttcccgtggc agggcgccag tgaaaatgat gcgatccgaa gctttcgatt tgttagctag
     1261 ctcttctagg acctcgcgaa actcaaagtc tccgacaaac aacagtttgg ccttaggccg
     1321 gttcttgatg actttttcaa aagctttcaa aagcaatacc aagttcttct cagagcctag
     1381 tcgaccgaca aagccgataa cttcgtcacc gggctcaatc tgccaggctg accgaaaatc
     1441 ctccacttgc ttcggtgtcg gctttttaat cgcattgaca ccattgggaa gtaagcttac
     1501 ttggtaggtg tagtgatcgg cgtactgcca agattccaat tgcttctggc tctttttgga
     1561 cagagcgata acggcatcgc atttggagta gattagcgtc accatccgct caacaatgtc
     1621 gatattccaa gctaccgagc ctcggcgtgg ccggtatagt ttcatgattt ctttaagatc
     1681 acgcccgttt agcttgacta ctaccggcag gaaagcgatc agggtcaata ggccgggcaa
     1741 aagtagagtg tcacggtagt gttcgacgta ttcccttaga tcggtcgaat gctgagcaat
     1801 cagcggcgta tctgtcttgt aagctgcata gaccgccatt aggccaactt ggccaggatc
     1861 gaagaagtgg atgacgtcta gatcaagctc acgaatttga cgcaacacgc gcggcgggaa
     1921 gaacagggac gtatcgtagt cttcataaaa tgctccctta actgaaggga agcggatgat
     1981 ccggtcatct tccttaaaga cccgggcatg cttagtccgc cgaatcgatc gggctggaca
     2041 aaagacgtag acctcgtgac cggcggcctc tagctctcgt ttgaccg
//
LOCUS       DASSBW010000094         5854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_875491, whole genome shotgun sequence.
ACCESSION   DASSBW010000094 DASSBW010000000
VERSION     DASSBW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875491"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..317
                     /locus_tag="VFG56_02705"
                     /pseudo
     CDS             33..317
                     /locus_tag="VFG56_02705"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
     gene            complement(314..1621)
                     /locus_tag="VFG56_02710"
     CDS             complement(314..1621)
                     /locus_tag="VFG56_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622821.1"
                     /translation="MEVAPYFVRPSVGAFERGFSTEKTEEVIEELEDPSGTEQPIATV
                     EQLDGDIDWFVESLDEVEVIETANEAEPSQDETELPRSLGTEALTWFEAELINEEEPR
                     FERRTFSFSDEELIPTRPTEEVVSSQPALEGVAVNEAIAIDEESQYKLETKNGSLSPP
                     KEIEATLAQEAIVTIEQIPKEEIRQHAEAKIPEEPKREASRPNHENRPNSLTSRHQSE
                     KAVNNAETETPPGSATGVDHQTEAEVSPDDSRPDTQTAPKQVNPVAGQLETANRVPEV
                     EAPTESPYATSVESVEEIITYVEHRIEQIVTQRTVSEIATKPERPTASPRRNTITPPR
                     PIIVYQSAPTKMDGGRINSAPATAALPETGNDTEETDSVTKIVQEFMQKLDQSSITEQ
                     DAYLTLRRLRQRIAALQGLAEVLRQQLGRQAIRLSKVPELSFA"
     gene            complement(1972..2048)
                     /locus_tag="VFG56_02715"
     tRNA            complement(1972..2048)
                     /locus_tag="VFG56_02715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2012..2014),aa:Arg,seq:tcg)
     gene            2298..3131
                     /locus_tag="VFG56_02720"
     CDS             2298..3131
                     /locus_tag="VFG56_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622822.1"
                     /translation="MEPNKQPSFDPSGNQNLPDQNSNSGPPANDKNQTIAGPSETVGQ
                     TGEAATPPDAPATDQPETTNNPAAASEPLAMASFQPMTDAPAVTTAASADSDQAFSSS
                     PSLSSASNSTADSKDNEHSYLSAFLLTTTFLAIFGVRSFYSGDKVGGWIRLALGGGGI
                     VLEFIGMLTAPVLAIIGGLMFIVAAVWAIIDFFYTFFALRSGKQGVSVSQSDRHWATF
                     IFSLTIAAVVAYAVVSAFVFQSLSNSLDLNSGVDSSFDASFDSSLDSRPSSNSFKFQT
                     D"
     gene            3172..4191
                     /gene="murB"
                     /locus_tag="VFG56_02725"
     CDS             3172..4191
                     /gene="murB"
                     /locus_tag="VFG56_02725"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET6622823.1"
                     /translation="MHSRHNVPLSELTTMRLGGLARLVVTVESTDELKENIEACAASE
                     QPFFVLGGGSNVIARDKEFAGTVILNRIHGFEILDDNETSTTVKIGAGEMWDEVVART
                     VGMGLSGIEALSAIPGTTGATPVQNVGAYGQEIADTLIELEAIDTETMELVTLANKDC
                     VFSYRNSIFKDPTTRHHIITSITLSLSKSKMKPPFYASLQNYFEQNDITDYSPASVRQ
                     AVIDIRSVKLPDPTKVANTGSFFKNPIVGSQLAEKLAEDYPEMPQFPVAGVGVKLAAG
                     WLIEQVGLKGYANHGFTTSPDNALVIINQSADKYSELELFKEEIIGKVQTKFGVKLEQ
                     EPETL"
     gene            complement(4188..5465)
                     /gene="glyA"
                     /locus_tag="VFG56_02730"
     CDS             complement(4188..5465)
                     /gene="glyA"
                     /locus_tag="VFG56_02730"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET6622824.1"
                     /translation="MNDDTVSNLIEQEAERQRDGLELIPSENYVSREVLQAMGSVLTN
                     KYSEGYPGRRYYGGQEFVDQVEQLAIDRAKQLFAADHANVQPHSGAPANEAVYSAWLE
                     PGDTVLAMDLSHGGHLTHGAPVTRSASLYNFVRYKMKDLETGEIDYDDLRELALTHRP
                     KIILAGFSGYPRNLDYAKFAAIGQEVGALLMADMAHIAGLIAGGVADNPFDYGFHVIT
                     TTTHKTLRGPRGGLILSKGVVSNPLKAPEKTLENIPTLIDRAVFPGMQGGPHEHIIAA
                     KAVCFYEALQPEFKVYTEQILKNAERLADELKSRGFQLITGGTSNHLILADVKSSFGI
                     DGKVAEEALDKIGLTVNKNAIPDDELPPFRPSGIRLGTPAMTTRGLKEDDMARIAEWM
                     KLAIENRDNEAKLAEFKSEVTTFARKFPLPSDN"
     gene            5533..>5854
                     /locus_tag="VFG56_02735"
     CDS             5533..>5854
                     /locus_tag="VFG56_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6622825.1"
                     /translation="MTNFKQRLGVIIQEARISRNMTQSQLADRLSTSQSAINRIEKGG
                     QNVSLEMLARISEVLDSEIVSLNQSGAINFRVEGGKKLTGAIEVKTSKNAAVALLCAS
                     LLNRG"
BASE COUNT         1422 a         1507 c         1361 g         1564 t
ORIGIN      
        1 atatgcacga ttgctaggtt cgaacgggca acgactggtt agccaccgaa gctccgaagt
       61 tgctgaattc cactgctttt aaaaacagtg actcgctgtt gatcgtgact tttgacgaag
      121 ccgagagtgg tgatgaagtc aaccgtatcc ccactatcct agtcggaagg tcagttaaac
      181 aaaactttac ttccgaccaa ccctatagtc attactcact cctgcacacc acagagtcgg
      241 cctggggcct caagccacta accaaaaacg acgctaacgc cccgctgatg gacgagttct
      301 ttacgacctc gcactaggcg aagctaagtt cgggtacttt tgataaccga atagcttgac
      361 gtccaagctg ctggcgcaat acttcggcca aaccctgcaa ggcagcgatc cgctgtcgta
      421 accgccgcaa cgtgaggtaa gcgtcctgct cggttataga tgattgatca agcttttgca
      481 taaactcttg tacaattttc gtcactgagt cggtctcttc ggtgtcatta ccggtttccg
      541 gcaaagcagc tgtggctgga gctgagttaa ttctaccgcc atccatcttc gttggagcag
      601 actgataaac aatgatcggc ctaggtgggg ttatggtgtt gcgtctaggt gaggctgtcg
      661 gacgctcggg cttagttgca atctcagaaa ccgttctctg agtaactatc tgttctattc
      721 ggtgctcgac gtaagtaatg atttcttcga ccgactcgac gcttgtggcg tacgggctct
      781 cggttggcgc ttcaacctca ggtacccgat ttgcggtctc taactgtccg gctaccggat
      841 taacctgttt aggcgcagtt tgggtatctg gtcgagaatc gtctggcgag acttcagctt
      901 ctgtctggtg atcgactccg gttgctgatc ctgggggtgt ttccgtttca gcgttgttca
      961 ctgccttttc tgattgatgc ctagaagtaa gcgaatttgg cctattttca tggttaggcc
     1021 gagaagcttc tcgcttgggc tcctctggta tcttagcttc cgcatgctgt ctgatctctt
     1081 ctttaggtat ttgctcgatt gtaactatcg cctcttgcgc taacgttgct tcaatttctt
     1141 ttgggggtga tagagatccg tttttagtct cgagcttgta ttgcgattct tcgtcaatcg
     1201 caatcgcttc atttaccgct actccctcga gagctggttg agacgaaact acctcctccg
     1261 tcggtcgtgt cggtatcagc tcttcgtcag aaaacgaaaa tgttcttcgt tcgaaacgcg
     1321 gttcttcttc gttgattagc tctgcctcaa accaagttag tgcttcagta cctaagcttc
     1381 ttggtagctc tgtctcgtcc tgacttggct cggcttcgtt tgctgtttct atcacttcta
     1441 cttcgtctag agattcgacg aaccaatcga tatcaccgtc cagctgttct actgttgcta
     1501 ttggttgttc ggtgccagaa gggtcttcca gctcctctat gacctcttcg gtcttttcag
     1561 tcgaaaaacc tctttcaaat gcgcctacac taggccgcac aaaataaggt gctacttcca
     1621 tcgttccttc ctctaatcaa tccttgatga cttttagtta gctgatttga atctacttta
     1681 agaagatagc tttcatcgtt tgttgctatc tatttgatag atattatggg aagcggttca
     1741 agctattaga agatttttaa tgttgtaaat tttatataat tcataacttc aaatcttggc
     1801 ttatttctcc tggtacaccc ggcaagatta ttaggatata acctgcagcc atgctcggtg
     1861 taagcacctg cgcgtggctt tgcttatcga acttgggctt cgcctaccaa gttctcatct
     1921 tgccgtgaat tataaccacg aaaaaaccca acattaagtt gggttatttc gtggtacacc
     1981 cggcaagatt cgaacttgcg acacctggtt ccgaagacca gtgctctaat ccactgagct
     2041 acgggtgcgt aacatgagac aaaccgaggt ttttctcatc gcctagtcgg cgcagccgag
     2101 tttagctaaa actaaaccga gctaggctgc ccttttcctt atgcgactcg actattataa
     2161 cagatgtgat gcaaccaaat taaaattaaa ctgctctttt ctttagcgga cgaagctatt
     2221 atagcaacgg gtttggtatt cataaatcac tgtgctaacc tgatagtcaa ataattaaga
     2281 caaaaaagga caacaatatg gagccaaata aacagccgag ctttgacccg tcgggtaacc
     2341 agaacttacc agatcaaaac tcaaactccg ggccaccggc aaacgacaaa aaccagacta
     2401 ttgccggacc tagtgagaca gtcggccaaa ccggtgaagc ggctacgccg cctgatgcac
     2461 cggccactga tcagccggaa actacgaaca atccggcagc tgctagtgag ccgctggcaa
     2521 tggctagctt ccagccgatg accgacgctc cagctgtgac gactgcggct tcggccgaca
     2581 gcgaccaagc atttagtagt agtccttctt tgagcagcgc cagtaactca actgccgact
     2641 ccaaagataa cgaacacagt tacttatcgg cctttttgct gaccaccacc ttcctagcta
     2701 tttttggcgt gcgatcattt tacagtggcg acaaagtcgg tggctggatt cggctagcgc
     2761 ttggcggtgg tgggattgtg ctagaattca tcggcatgct gactgcccca gttctagcga
     2821 ttatcggtgg gttgatgttt attgtagccg ccgtctgggc cattatcgac tttttctaca
     2881 ctttcttcgc gctacgtagc ggcaaacagg gtgtcagcgt cagccaatcg gaccgtcact
     2941 gggcgacctt tatctttagt ttgaccatcg ctgccgtagt cgcctacgcc gtggtatccg
     3001 ccttcgtttt tcaaagcttg tcgaacagtc tagatcttaa ctccggtgtc gactctagtt
     3061 ttgacgctag cttcgattct agcttggact ctcgccctag ctccaatagt ttcaagttcc
     3121 aaaccgacta gcgtaccgtc tagagctagt cgctatcgta taatggtaat aatgcacagc
     3181 cgtcacaacg tacccttatc tgaactaact actatgcgcc tgggcggtct cgctcgtcta
     3241 gtcgtgactg tcgaatcaac cgacgaactt aaagaaaaca tagaagcctg cgctgctagt
     3301 gaacaaccgt tctttgttct cggtggcggc tccaacgtta ttgctcgcga caaggaattt
     3361 gccggtaccg tcatcctaaa tcgcattcac ggcttcgaga tcttggacga taacgaaacc
     3421 tcaactactg tcaaaatcgg tgccggtgag atgtgggatg aagtcgtggc caggactgtt
     3481 ggtatgggac tttccggcat cgaagctctt tcagccatac cgggtaccac cggagctaca
     3541 ccagtccaaa atgttggtgc ttacggccaa gagatcgccg acacgttgat tgagctcgaa
     3601 gctatcgata ctgaaacgat ggagctggtg acgctagcaa ataaagactg cgttttctct
     3661 taccgaaaca gtatcttcaa agacccgacg acacgtcacc acatcatcac ttcgatcact
     3721 ctgagcctca gtaagagcaa gatgaagcca cctttttatg catccctgca aaattatttt
     3781 gagcaaaatg acataactga ctattcacca gctagtgtac gccaagccgt catcgatatc
     3841 cgatccgtca aactacccga ccccaccaag gtggccaata ccggatcatt tttcaagaac
     3901 ccgatcgtcg gatcgcaact tgctgaaaaa ctagccgaag attaccctga gatgcctcaa
     3961 tttcctgtag ccggcgtcgg tgtcaaatta gccgccggct ggctgatcga acaggtcggc
     4021 ctcaaaggct acgccaatca tggcttcacc acttcaccgg ataacgccct agtcatcatc
     4081 aatcagtcag ccgataaata ctccgaacta gaacttttta aagaagaaat catcggaaaa
     4141 gttcagacta aattcggcgt caaactagaa caagagccag aaaccctcta gttgtcgctc
     4201 ggcagcggaa acttacgagc aaaagttgtg acttcacttt taaattcagc tagtttagct
     4261 tcattgtcac ggttttcgat agccaatttc atccattcgg caatccgtgc catatcatct
     4321 tccttaagtc cgcgcgtagt catggccggt gtaccgagcc ggataccact tgggcggaat
     4381 ggcggcagct cgtcgtccgg tatggcgttt ttgttgacag tcagaccaat cttgtcgagc
     4441 gcctcttcgg ctactttgcc gtcgatgcca aagcttgatt ttacgtcggc tagtatcaag
     4501 tgattcgacg taccgcccgt aatcagctgg aagccacgag actttagctc gtcggctaag
     4561 cgctcggcgt ttttcaaaat ctgttcggtg taaaccttga actccggctg caaggcttca
     4621 taaaaacaaa cggctttggc ggcaatgata tgctcatgtg gtccaccttg catgccaggg
     4681 aaaaccgccc gatcgatcaa tgtggggata ttttccagtg ttttttccgg agctttcaaa
     4741 ggatttgaaa cgacgccttt gcttaagatt aatccgccgc gaggtccgcg taaggtcttg
     4801 tgagtggtcg tcgtgatgac atggaaacca tagtcgaacg gattgtcagc cacgccgccg
     4861 gcaatcagtc cggcgatatg ggccatatcg gccatcaata acgcaccaac ttcctgcccg
     4921 atagcggcaa atttagcgta atctaaattc cgtgggtagc cggaaaagcc agccaggatg
     4981 atctttggac ggtgtgtaag tgccagttcg cgtaaatcgt cgtagtcgat ctctcctgtc
     5041 tcgagatctt tcatcttgta gcgaacgaag ttataaagag aagccgagcg ggttaccgga
     5101 gcgccatgcg tcaggtgacc accatgagat aagtccatgg ccagtactgt gtcacccggc
     5161 tctagccatg cagagtaaac cgcctcgttg gccggagcgc cagagtgcgg ctgaacattg
     5221 gcgtgatcgg cggcaaacaa ctgtttagct cgatcgatgg cgagttgctc gacctgatcg
     5281 acaaattctt gaccaccgta gtatcggcgg ccaggatagc cttctgagta cttgttggtc
     5341 aagacgctcc ccatagcctg aaggacttcc ctagagacat agttttccga cggtatcaac
     5401 tccagaccat cacgctgacg ctcggcttct tgctcgatta ggttagaaac agtatcgtca
     5461 ttcatctacc ccagtataca taatgtgtaa aatactatag ccattatatc acttgtgata
     5521 taatcaattg gtatgacaaa tttcaaacag cgcctcggcg ttatcatcca agaagcccgt
     5581 atatcaagaa atatgactca atctcagcta gccgatcgct taagcacctc acaaagcgcc
     5641 ataaatcgca tcgaaaaagg tggtcaaaac gttagccttg agatgttagc taggattagc
     5701 gaagtactcg attccgagat cgtttcgctc aaccagtcag gcgccatcaa cttccgcgtc
     5761 gaaggtggca aaaaactgac cggtgcgatc gaagtcaaaa ctagtaagaa tgccgctgta
     5821 gccttgcttt gcgcctcgct actgaaccgg ggca
//
LOCUS       DASSBW010000095         7518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_157007, whole genome shotgun sequence.
ACCESSION   DASSBW010000095 DASSBW010000000
VERSION     DASSBW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157007"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFG56_02740"
     CDS             <1..497
                     /locus_tag="VFG56_02740"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622826.1"
                     /translation="LVDKKQPILLAAPDSDAGDYKVLSQLPKLLDEFLPGNHTMTQPN
                     ELHELTWPIVEQVVCQPKAAAAAEKFQQLVGPDKSSAKFSDIEAAAEAGRVDSLLIGM
                     FDITADSVSDSKRRDLLKLTFPSVYERLSQLVQQVIAQGGRIIGMQTDTMPASTTVAA
                     LYRY"
     gene            570..893
                     /locus_tag="VFG56_02745"
     CDS             570..893
                     /locus_tag="VFG56_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET6622827.1"
                     /translation="MIGRFLAGLINFFVAVVEIFLGLRVVLRLFGANPDNGFVQWIYD
                     SSGVLMEPFRGIFPTETIAPDHVVDFSALFAMLVYALIGMAFLALATWLTPRDNVVVT
                     KKARK"
     gene            complement(953..1354)
                     /locus_tag="VFG56_02750"
     CDS             complement(953..1354)
                     /locus_tag="VFG56_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622828.1"
                     /translation="MIRGRVVNGVQPGNDQSSAEAQSQAEQLWASRQRDTWYMAIVDR
                     MDGLVLLCSDPKPLEAGADDIRGYHFQNAVAGYSGAGPITTVKILTLFGFGRFKDLFR
                     KVARGDQEAFYGFVKVDGEVVFTKSPQDLLP"
     gene            1493..1888
                     /locus_tag="VFG56_02755"
     CDS             1493..1888
                     /locus_tag="VFG56_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622829.1"
                     /translation="MGQRYGVSHLHLGLPLDLAMTGSFLDRDGVETLLRAGGWIRLRL
                     MASQAKKFGGSVLYLPAFHLDIESALNRMQIVLRIWMSPRLAASTMPGVKYNSERKPS
                     RWSNRKLGRLAKAASSELKRALLFVVMYL"
     gene            complement(1907..2887)
                     /locus_tag="VFG56_02760"
     CDS             complement(1907..2887)
                     /locus_tag="VFG56_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622830.1"
                     /translation="MRHYITRQRRGKGFSYQLDGQTLRDNEQLDRIKSLAIPPAWTDV
                     QIAVSPRAKVQATGRDQADRLQTIYNPSFRARQERAKFERMLDFAASLPAARAKVDKD
                     LSRRALDKRRVLAAVFRLLDTAYFRVGNPIYAKHHRSFGLTTLTRRHAEVKGDKIMFD
                     FVGKSGQQQRKTLTDRRLARLMRQLDELPGDQIFSYFDKSGEVRQLTSGDVNDYIRRV
                     TGGDFTAKDFRTWGGTLLATANLIKKPRPSDAKRRQKRVTACLKSVAKRLGNTPAVVR
                     SAYVHPELLERYLTSDDLSQAKLRVDSQPVERFCRSEESCLLELFRPSSS"
     gene            complement(2933..3017)
                     /locus_tag="VFG56_02765"
     tRNA            complement(2933..3017)
                     /locus_tag="VFG56_02765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2981..2983),aa:Leu,seq:tag)
     gene            complement(3177..3545)
                     /locus_tag="VFG56_02770"
     CDS             complement(3177..3545)
                     /locus_tag="VFG56_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622831.1"
                     /translation="MLIVLELRHLTWLCFYANIKIRYLDKQILGVVMAWYYWTLAWTL
                     FCAMAAAIQMMNDKRSLKISQHVWLYGSRFMTWLVVVPIITAVALICAPFFFIVYLMM
                     YGGMTRQEIREHKELARSYG"
     gene            3578..4876
                     /gene="hisS"
                     /locus_tag="VFG56_02775"
     CDS             3578..4876
                     /gene="hisS"
                     /locus_tag="VFG56_02775"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET6622832.1"
                     /translation="MANLSNQPYKGTRDYYPSDKRLQNYIFGVWQRVAERFGYEEYAA
                     PILEPLELYAAKTGQEIVNEQTYQFVDRGGRNVAIRPEMTPSIARMVAARSQELGYPA
                     RLYSIANFMRYERPQKGREREFWQLNLDLFGVDGVEADAEIIIVSDQIMREFGAKDDM
                     YTIRINNRRLINFMMADYLELDGADALAMIKLFDRKDKMNGEQFMAQAAGIFDGQNSE
                     EGLKKIQRLVSANSMADLPEDIRDSEAVEEVRELFSRLREAGVTNAKFDITLMRGFDY
                     YTGMVFEVFDNSPDNNRAMFGGGRYDGLVGLFGGDNVPTVGVAPGATTTEEFLRSHQL
                     LPDLKSTTDAVVIVIGDNLKSAQKLAASLRESGVKVAVDLTSRKTDKQIKSVVKMNVP
                     YMIFVGDHEVAESKYRLKDVADQSESSLGLDELVQAIKRA"
     gene            4916..6193
                     /gene="tig"
                     /locus_tag="VFG56_02780"
     CDS             4916..6193
                     /gene="tig"
                     /locus_tag="VFG56_02780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET6622833.1"
                     /translation="MKHTLKKTNDSHVSLSVELDKADLAIAKAQAVKNLAPSVKAAGF
                     RPGKVPADVAEKNLDPNLLNQETAELAINTAINDAAVAEELRILDRPHIEMKEFVPEQ
                     SLNFTAEVDVLPDIKLGDYKKLKIKAEKAEVSDKDIDEVIDRMRSGMAEKQAVEHEAK
                     NGDEATIDFVGKKDGEAFDGGSAQDYQLALGSNSFIPGFEEAIVGHKAGDKFDIPLEF
                     PKDYHAENLKGQKVVFEINLKAVNEIKLPEVNDDFAKKCGPFKTVEELKSDVKRELTA
                     QKDRQAEEKLKDDLIGELVKVSDVPVPDVLVDDQMKSIEQDARQNLMYRGQTLEQYIE
                     AQGFKDEADWKDKEVKPNAVRRVQAGLALSELSKLEKIDVSQNELEERLAQMKQQYPN
                     MTDQLDRPEARRDLANRVITEKTLDRLIELNQK"
     gene            complement(6230..6484)
                     /locus_tag="VFG56_02785"
     CDS             complement(6230..6484)
                     /locus_tag="VFG56_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622834.1"
                     /translation="MLSAEDLRELCPADCPILQGLADKRAETIRHLVLEQDNMSKPQR
                     DEIWQKADWEDADARELARTCLKHTFGFGYPQHCTSFPDK"
     gene            6593..7189
                     /locus_tag="VFG56_02790"
     CDS             6593..7189
                     /locus_tag="VFG56_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET6622835.1"
                     /translation="MTKPRSFLVPTVVEKSYDGERAFDIYSRLLKERIIFLGEDVNEH
                     TANIVVAQLLHLAYEDPDKDISLYINSPGGSVYDGMAIYDTMQFVKPDVATYGIGLQA
                     SMGAFLLSSGAKGKRSVLPHSRVMIHQPSSGTQGKVSDQEITLRESIRIKRMLNEILA
                     KNTGQKMSKIEKDVDRDFWMSADEAVTYGLADKIVKKI"
BASE COUNT         1899 a         1900 c         1989 g         1730 t
ORIGIN      
        1 accttgtcga caaaaagcag ccgatccttc tggcggcacc cgatagtgac gctggcgact
       61 ataaggtgct gagtcaacta cctaagctac tggatgaatt cttgccgggt aaccatacga
      121 tgacgcagcc aaatgagctg catgagctga catggcctat tgtggagcag gtagtttgtc
      181 aaccgaaggc cgcggcggcg gctgagaagt ttcagcagtt ggtaggtcct gataagtcgt
      241 cggccaagtt cagtgacatc gaggcggccg cggaagccgg tcgcgtcgat tctctgctta
      301 tcggcatgtt cgatatcacc gccgacagcg tcagcgacag taagcggcgt gatttgctca
      361 aactgacctt tccaagtgtt tatgaacgac tcagtcagct agtccagcaa gtcatagccc
      421 agggcggtcg aatcataggt atgcagactg ataccatgcc ggcttcgacc accgtagcag
      481 cgctgtaccg ttactaggag aagtttacgt ccgtagttgg tttgtgttat aaccggtaac
      541 ataaatctaa caccgaggag gaattaaaaa tgatcggtag atttctagcc ggacttatca
      601 actttttcgt agccgtggtc gagattttct tgggcctacg tgtcgtactg cgtctgtttg
      661 gcgctaaccc ggacaacgga ttcgtgcagt ggatctacga ctctagtggc gtgctgatgg
      721 agccgtttcg tggtattttc ccgaccgaga caatcgcacc tgaccatgta gtcgactttt
      781 cggctctgtt cgcgatgctg gtctacgccc tcatcggcat ggccttcttg gcgctggcta
      841 cttggttgac accacgagac aacgttgtcg tcaccaaaaa agctcgtaaa taatcaaaac
      901 atttgaataa aagaaaaccc ctcgtaccgg tatacggacg aggggcgttc ttttacggca
      961 gtagatcttg cggtgacttg gtgaagacaa cttcgccgtc cactttgacg aaaccataga
     1021 aggcctcctg atcaccgcga gctaccttgc gaaacagatc cttgaagcgg ccgaaaccga
     1081 acagggtcag aatcttgacc gtagtaatcg ggccggcgcc cgagtatccg gcgacggcgt
     1141 tttggaagtg gtagccacgg atgtcgtcgg caccggcttc gagcggcttg gggtcactgc
     1201 atagcaacac cagaccatcc atgcggtcga cgatggccat ataccaagtg tctcgctgcc
     1261 gactagccca aagttgctcg gcctggctct gggcttcggc gctagattgg tcgttgcccg
     1321 gctgcacgcc gttgacgacg cggccgcgta tcataacagc cactgtagcc tcctaggttg
     1381 atgtgtaaaa gaacgtaggg atacattacc ataaaaacta taaaaaatca atgaaacagc
     1441 ccacaccaat cagggtgtgg gctcttgttt cagctctttt taagcatcgt ggatggggca
     1501 gcggtacggc gtatcgcact tgcacttagg attaccgttg gacttggcca tgacaggctc
     1561 cttcctcgat cgggacggtg tcgaaacact gctacgagct ggcggctgga tacgcctgag
     1621 acttatggcg tcccaagcga aaaagtttgg tggctcggtt ttgtacttac cggccttcca
     1681 tctggatatc gagtcggctt tgaatcgcat gcagatcgtt ttacgcatct ggatgtcgcc
     1741 tcgtttggcc gcgtccacca tgccgggcgt taagtacaac tcggagcgaa agccgagtcg
     1801 gtggtccaac cgaaaattgg gccgtctggc caaagctgcc tcctcagagc tgaaacgtgc
     1861 actactcttt gtagttatgt atttataaca gaaataagca taatcttcaa gatgaggaag
     1921 ggcgaaacag ttctagtaaa caactttctt cacttctgca aaatcgttcg accggctggc
     1981 tatcgacgcg cagcttagct tggctcaggt cgtcgctagt cagatagcgc tctaatagct
     2041 cggggtggac ataggcgctt cttactaccg ccggagtatt gccaaggcgc ttggctacgg
     2101 atttgagaca agctgtgacg cgcttttgcc ggcgtttagc gtctgagggc cgtggcttct
     2161 tgatgagatt ggcggttgcc agtagggtgc cgccccaggt tcggaagtcc ttagctgtga
     2221 aatcaccgcc ggtaactcgt ctgatgtagt cgttgacatc gccactggtc aactgacgca
     2281 cttcacccga cttgtcgaag taactaaata tctggtcacc cggtagctcg tcgagctgac
     2341 gcatcaacct ggccagacgc cggtcggtca gggtcttgcg ctgttgttga ccgctcttgc
     2401 cgacgaaatc aaacatgatt ttgtcacctt tgacttcagc gtggcgacgg gtcagggtgg
     2461 tgagaccaaa cgaccgatga tgcttggcat agatggggtt gccgacgcga aagtaagctg
     2521 tatcgagcag gcggaagaca gcggctagta cccggcgttt atcgagcgcc cgccgcgaca
     2581 agtctttatc aaccttggcg cgagccgctg gcagactagc ggcaaagtcg agcatccgct
     2641 caaacttagc tcgttcctgt cgggctcgaa aagacgggtt gtagatagtt tgcagccggt
     2701 cggcttggtc gcggccggta gcttggactt tggctctagg gctgacggca atctggacat
     2761 cggtccaagc cggcgggata gctaaagact tgatgcggtc taattgctcg ttgtcacgca
     2821 aagtctggcc atccagctgg taactgaagc ctttgccgcg acgttgcctg gtgatgtagt
     2881 gtctcatact atcattttac aaaaagccga ccacaaggtc ggcatattgt tttggtgcgc
     2941 gagagaggac ttgaacctcc acgcccgaag gcacatgctc ctaaggcatg cgtgtctacc
     3001 aattccacca ctcgcgcatg aaattagcag agtttattgt agcagattga ctagttctaa
     3061 gtgaaaaaag ccccgacttt ttgaaaagtt cggggcgctg agctcggaaa agctcgcgat
     3121 acctagtcta gtcgaccggc gctccgggtc gttcccggag cgccatagcc acagcatcag
     3181 ccgtagcttc gtgcgagctc tttgtgctcg cgtatctcct ggcgcgtcat ccctccgtac
     3241 atcatcaagt agacgatgaa gaagaaaggg gcgcagatga gcgcgacagc tgtgatgatc
     3301 ggtacgacca ccagccatgt catgaaccta ctgccgtaca gccagacgtg ctgactgatc
     3361 ttgagtgacc gcttgtcatt catcatctgg atggcggctg ccatggcgca gaacagtgtc
     3421 caggccagtg tccagtagta ccaagccata actactccta ggatttgctt gtcaaggtat
     3481 cggatcttta tattagcata aaagcataac caagtcaagt gacgtaactc gagcactatt
     3541 aacatttacc cctgtttgtg acatacttaa ggcaattatg gccaatctat caaaccaacc
     3601 ctacaaaggt actcgtgatt actatccgtc agacaagcgc ttgcagaact atatcttcgg
     3661 cgtttggcag cgtgtggctg agcgttttgg ctacgaggag tacgcagcac cgatcctaga
     3721 gccactcgag ctatatgccg ctaagaccgg tcaggagatc gtcaacgaac agacctatca
     3781 atttgtcgat cgtggtggcc gcaacgtagc catccgcccg gaaatgacac cgtcgatcgc
     3841 ccgcatggta gcggctcgga gccaagaact tggttacccg gcccggttgt attctatcgc
     3901 caacttcatg cgctacgagc gtccgcaaaa aggtcgcgaa cgcgagtttt ggcagctaaa
     3961 cctagacttg ttcggcgtcg acggtgtcga agccgatgcc gagatcatca tcgtatctga
     4021 tcaaatcatg cgcgagtttg gcgcgaaaga tgacatgtat accatccgta tcaacaaccg
     4081 ccgcttgatc aacttcatga tggccgacta tctggagcta gacggtgccg acgctctcgc
     4141 tatgatcaag ctatttgacc gcaaggacaa aatgaacggt gagcagttca tggctcaggc
     4201 ggccggcatc tttgatggcc agaactcaga agagggcttg aagaaaatcc agcggttagt
     4261 cagcgccaac tcgatggctg acttgccgga agatatccgt gattcggaag cggtcgagga
     4321 agtccgcgaa ttgtttagtc ggctacgaga agccggtgtg accaacgcca agtttgacat
     4381 cacactgatg cgaggcttcg attattacac cggcatggtc ttcgaagtat ttgataattc
     4441 gcccgacaac aaccgagcca tgttcggtgg cggccgttac gacggcttgg ttggcttatt
     4501 tggcggcgac aatgtaccga cagtcggagt ggcaccgggc gccacaacca ccgaagagtt
     4561 cttgcggagc caccagctat tgcccgacct caaatcgact actgatgctg tcgtcatcgt
     4621 catcggcgat aatctaaaat ccgctcaaaa actagctgct tcgctcagag agagcggcgt
     4681 caaagtggcg gtcgacttaa ctagccgtaa gacggacaag caaatcaaat cggttgtcaa
     4741 aatgaacgta ccgtacatga tctttgtcgg tgaccacgaa gtagccgaat ccaagtaccg
     4801 gctaaaagac gtggctgatc aatccgagag cagcctaggc ctagatgagc tggtccaagc
     4861 tatcaaacga gcttaagttt gagagcttcc tctgattttg ctacactagg cgcttatgaa
     4921 gcacactctt aaaaaaacca atgattccca tgtctcgctt agcgttgagc tggacaaagc
     4981 cgacttggcc atagccaaag cccaagccgt caaaaatcta gcccctagcg tcaaagccgc
     5041 cggctttcga cccggcaaag taccggctga cgtagccgag aaaaatctcg acccaaatct
     5101 acttaatcaa gagacggccg agctagccat caataccgct atcaatgatg ctgccgtagc
     5161 cgaagaactg cgtattttgg atcgaccaca catcgagatg aaggagttcg tgccggaaca
     5221 atcgcttaat tttacggctg aggtcgatgt tttaccggat atcaaactcg gtgactacaa
     5281 gaaactgaag ataaaagccg aaaaggcaga ggtcagcgat aaggatatcg acgaggttat
     5341 cgaccggatg cgctccggta tggctgagaa acaggcagta gagcatgaag ccaaaaacgg
     5401 cgatgaggcg accatcgatt ttgtcggcaa aaaagacggc gaagcttttg acggtggtag
     5461 tgcccaggat tatcaattgg cacttggatc aaactctttc atcccaggct tcgaagaagc
     5521 tatagtcggc cacaaggctg gtgataagtt tgatataccg ctggaatttc ccaaagacta
     5581 ccacgccgaa aacctcaaag gccagaaggt ggtgtttgag atcaatctaa aagcggtcaa
     5641 cgagatcaaa ctaccggaag taaatgacga ctttgccaag aagtgtggcc cgttcaagac
     5701 cgtcgaagaa cttaagagtg acgtcaagcg tgaactaact gctcaaaaag accgacaagc
     5761 cgaagagaaa ctaaaagacg acttgatcgg tgagttggta aaagtctcgg acgtcccagt
     5821 gccggatgta ttagtcgacg accaaatgaa gtcaatcgag caagatgctc gtcagaactt
     5881 gatgtaccgc ggtcaaaccc tggaacaata catcgaggct caaggcttta aggacgaggc
     5941 tgactggaaa gacaaggaag ttaagccaaa tgccgtccgc cgggtacagg ctggcctagc
     6001 gctgtctgag ctatccaaac tggagaagat tgacgttagc caaaacgaac ttgaagaaag
     6061 gctagctcaa atgaagcagc agtacccgaa tatgaccgat cagttggatc gtcccgaagc
     6121 tcggcgcgat ctagccaacc gagtaatcac cgagaagacg ctcgaccgat tgatcgaact
     6181 caatcaaaaa taagtccttt cgatagagag cgccccgtcg gggcgttttt tatttgtcgg
     6241 ggaagctggt gcagtgctgg ggatagccga aaccgaaagt gtgcttgagg caggtcctag
     6301 ccagttcgcg cgcgtctgca tcttcccagt cagctttttg ccagatctca tcacgctgcg
     6361 gtttagacat gttgtcttgt tctagtacca aatggcgaat agtctctgct cgcttgtcgg
     6421 ctagaccctg gaggatgggg caatccgcag gacacaactc gcgcaagtct tcggccgaca
     6481 acatattagc ttaattaaac gggggaatag tcagtatgtc aaatagatgg aatgagcctt
     6541 gtttttggca ctctaacttg acgagtgcta atgcgcagcg tagactagaa ctatgacaaa
     6601 accacgtagc tttttagtac caaccgtcgt cgaaaagagt tacgacggcg agcgagcttt
     6661 cgacatatat tcgcgcctct taaaggagcg catcatcttc cttggcgaag acgtcaacga
     6721 acacaccgcc aatatagtcg ttgcccaatt gctgcatctc gcctacgaag atccggacaa
     6781 ggatatatcg ctctacatca actcacctgg cggcagtgtc tatgacggca tggccatcta
     6841 cgatactatg cagttcgtca aaccagatgt cgctacctac ggtatcggct tgcaagccag
     6901 tatgggagct tttctgctca gttccggtgc caaaggtaag cgctcagtct tgccgcactc
     6961 ccgcgtcatg attcaccagc cgagctctgg cacccaaggt aaagtatccg atcaagagat
     7021 cacactgcgt gaatcaatcc gcatcaaaag gatgttgaac gagattctgg ccaagaacac
     7081 cggtcagaag atgtctaaga tcgaaaaaga tgtcgatcgt gatttctgga tgagtgccga
     7141 tgaagcggta acctatggtc tagccgacaa aatcgtcaaa aagatctaaa ctttatctac
     7201 tttttatcta cttgagacca taattaccaa tttcagccac cgttcacgcg cggagggtaa
     7261 gctgcctgag ctgcaaaaga gatctttgca tcttcagcag cttaccgtac cggctccact
     7321 taggaatggt gagtgcctaa aattggaaat tacggtcgct ttttgttgac attgtccctg
     7381 tcggcagcta tagttgtagg taatgaagtc agtgtaagct tgggtattta tgcacgcacg
     7441 ccatttggcg gggcgttttt gtgtgcagag cccaaagcta gtagatcttc ggcgccggta
     7501 tagccttcaa cccaagcg
//
LOCUS       DASSBW010000096         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1220098, whole genome shotgun sequence.
ACCESSION   DASSBW010000096 DASSBW010000000
VERSION     DASSBW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220098"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1221)
                     /gene="ruvB"
                     /locus_tag="VFG56_02795"
     CDS             complement(211..1221)
                     /gene="ruvB"
                     /locus_tag="VFG56_02795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET6622836.1"
                     /translation="MIERIVEPDATPDEAEEIFENALRPKDFVNYIGQERLKNNLKLA
                     IKAAKKRSEPLDHVLLYGPPGLGKTTLASVIANEMGAQIRVTSGPAIERAGDLASLLT
                     NLTDGDILFIDEIHRLHRTVEEVLYSAMEDFKIDIMLGKGPSARSLRLDVPKFTLIGA
                     TTRTGALAAPLRDRFGHIHRLEFYKPEEISDIIARTAGILGTNIKPDEALTLAHRSRL
                     TPRIANRLFKRVRDYADVNGDGIIDSVNITQALELLEIDELGLDPADRLMLGAIIDNY
                     GGGPVGVETLSALISEERSTIEDFLEPYLMQVGLLERTPRGRKATPKAYKHLGKTRSS
                     DR"
     gene            complement(1218..1793)
                     /gene="ruvA"
                     /locus_tag="VFG56_02800"
     CDS             complement(1218..1793)
                     /gene="ruvA"
                     /locus_tag="VFG56_02800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6622837.1"
                     /translation="MIATLTGKVTETGSGWLVLDISGVGYEVFTTNDVLSKLGLGSEA
                     KLYVHDHIREQSRDLFGFLEVSTKQLFEQLLGVKNVGPKAALAILDIGPATGVRQAIA
                     GGDVKALTAAKGVGKRAAEQVIVELRDKVGALTGEGADAIVGRPGIDENDEAIQALIS
                     LGYSPQDASQALAKIDKTLSTEEKVKQALKS"
     gene            complement(1815..2294)
                     /gene="ruvC"
                     /locus_tag="VFG56_02805"
     CDS             complement(1815..2294)
                     /gene="ruvC"
                     /locus_tag="VFG56_02805"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET6622838.1"
                     /translation="MRIIGIDPGTGILGFGVIETLSSGKSKLIDAGVIRTPAHQADEL
                     RLETIYDELSGVIKQTKPEAMSVEKLFFARNVTTAMSVAQARGVVLLTGRQAGLDIAE
                     YTPLQIKQAVTGYGQADKKQIQQMVKTILGLKTVPKPDDCADAIAAALTHAQTMSSR"
     gene            complement(2312..>2905)
                     /locus_tag="VFG56_02810"
     CDS             complement(2312..>2905)
                     /locus_tag="VFG56_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622839.1"
                     /translation="LEQETGQWRWTMTPRPNQANHFELPEPSVSPVQTSDLKPCADNQ
                     FRNPATNRCKLKVSVTSQLKPCAANEFRNPATNRCKDKVSSSSSYVPCDENEKRNPKT
                     HRCRSVLGSKTSYVPCDPGEERNPATHRCRKVAGNPATQVGAVNPEQIINQSKQKDYG
                     NLILASTGALVVGYGLFEWRKELFDLGRKAFATFGHK"
BASE COUNT          673 a          741 c          721 g          770 t
ORIGIN      
        1 gactggttta ttggggtcgg tcgactttgg accagattga tttggttgca tgctggtatt
       61 atagctcaac gaagcgagag gaacgaaagc ttgctttcat tcccgagcga ggcagagctt
      121 cgacgtagtc atagcttagg cgaagcgaga ggaacgaaag cttgctttca ttcccgagcg
      181 aggcagagct tcgacgtagt cgtagcaatt ctatcgatca gatgacctag tcttgccaag
      241 atgcttatag gccttcggcg tcgctttgcg accgcgtggt gttcgctcga gcaagcctac
      301 ctgcatcaaa tacggctcaa gaaagtcctc gatcgtagat cgttcctcgc tgatgagcgc
      361 gcttaaagtc tctacgccaa ctggaccacc gccataattg tcgatgatag cgcccagcat
      421 caatcgatcg gccgggtcga gaccaagctc gtcgatttcc aaaagctcca aggcttgagt
      481 gatattaacg ctatcgataa ttccatctcc gttaacgtca gcatagtcgc gtactcgttt
      541 gaacagtcgg ttggcgatac gcggtgtcaa acgagaacgg tgagccaagg tcaaagcctc
      601 gtctggcttg atattggtac ccaagatgcc agcggtgcga gcgatgatat cactgatttc
      661 ttctggctta taaaactcta ggcgatggat gtgaccgaag cgatcgcgca atggcgccgc
      721 caaggcgcca gttcgagtag tggcaccgat caatgtaaac ttcggcacat ctaggcgaag
      781 tgaccgtgca ctcggcccct tgccgagcat gatatcgatc ttgaagtcct ccatggccga
      841 atagagcact tcttcaacgg tacggtgtaa acgatggatc tcatcgataa ataagatatc
      901 gccatctgtc aggttagtca gcaagctagc caaatcgccg gccctttcga tagctggacc
      961 actcgtcact ctgatttgtg cccccatctc attagcgatg acgctagcta gggtagtctt
     1021 accgagcccc ggaggaccat aaagcaggac gtggtcgagc ggttcactac gtttcttggc
     1081 agctttgata gccagtttca aattattttt taagcgttct tggccaatat agttgacgaa
     1141 gtctttgggc cgaagggcgt tttcaaatat ttcttcagct tcatccggcg tagcgtccgg
     1201 ttcgactatt cgttcgatca tgatttaagc gcttgtttaa ctttttcttc tgtcgacaaa
     1261 gtcttgtcta tcttagctaa ggcctggctt gcatcctgcg gactgtaccc aaggcttatc
     1321 agggcctgta tagcttcgtc gttctcatcg atacccggcc gaccaacgat agcatctgca
     1381 ccctcaccgg ttagggcgcc tactttatca cgcagctcaa cgatcacttg ttcggcagct
     1441 cgcttaccta cccccttggc agcagtcaaa gctttgacat caccaccggc aattgcctga
     1501 cgaacaccag ttgccggacc gatatctaag atagctaggg ctgctttcgg accaacgttt
     1561 ttaactccca gtaactgttc aaataactgc ttagtcgaga cttccaagaa gccaaataga
     1621 tcgcgcgact gttcacggat atggtcatgg acgtatagct tggcctcgct gcccagccct
     1681 aatttggata acacgtcatt ggtcgtgaaa acctcgtaac cgacaccact gatatccaag
     1741 accagccaac cggaacctgt ttcagtaact ttacctgtta gtgttgcaat catttgcctt
     1801 tagtatatct cgtgttagcg agatgacata gtttgggcat gggtcagagc agcagcaata
     1861 gcgtcagcgc aatcatcggg tttaggcacg gtctttagac ctaagatagt tttgaccatt
     1921 tgttggatct gttttttgtc ggcttgacca taaccggtga cggcttgttt gatctggagt
     1981 ggtgtgtact cggctatatc gagtccagct tgtcgaccag tcaacaatac tacgccgcgc
     2041 gcctgggcca cgctcatagc tgtagtgaca ttgcgggcaa aaaatagttt ctcgacagac
     2101 atagcctcgg gtttagtttg cttgatgacc ccactcagtt catcgtagat cgtctccagc
     2161 cgcagctcat cagcctgatg agctggagta cggatgactc cggcatcaat cagcttagac
     2221 ttgccgctgg atagcgtctc aattacaccg aaacctaata tgccagtccc gggatcaata
     2281 ccgatgattc tcatgagtct agtatagccg attacttgtg gccgaaggtg gcgaaggcct
     2341 tgcgccctaa atcaaacagt tctttgcgcc actcaaataa gccgtatcca actaccagcg
     2401 ccccggtact agctaagatt aagttgccat aatctttttg cttggactga ttgatgattt
     2461 gctcaggatt aactgcgccg acttgggtag ccggattgcc agccactttg cggcaacggt
     2521 gagtcgcagg attacgctcc tcccctggat cacaaggtac gtacgatgtc ttggaaccta
     2581 aaacagaacg gcaacggtgg gtcttaggat ttcgtttttc gttttcatcg cagggtacat
     2641 acgatgaact gctggaaacc ttgtctttac agcggttggt cgctggattg cggaattcat
     2701 tcgccgcaca gggctttagc tgactagtca ccgaaacttt caatttgcaa cgattggtag
     2761 ctggattgcg aaactggtta tcggcacatg gcttcaagtc gctagtttgc actggactaa
     2821 ccgacggttc cggtaattcg aagtggttgg cttggttcgg tcgtggtgtc atcgtccatc
     2881 gccactgacc cgtctcttgc tcaag
//
LOCUS       DASSBW010000097        12857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_909399, whole genome shotgun sequence.
ACCESSION   DASSBW010000097 DASSBW010000000
VERSION     DASSBW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909399"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VFG56_02815"
     CDS             <1..1635
                     /locus_tag="VFG56_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622840.1"
                     /translation="PAPADDDNDGVPNTQDECDGTAAGADVDANGCSADQRDSDGDGV
                     SDADDNCADTPSGTEVDDHGCPVDGGGDDGGNGGHNPPGNNGTIKIDDEAFDDHPNNE
                     PHVGCTFQVDFYGYDKGDYYADVNFELQSPTKNGRTLEVTSGDLDPFIGEDDASGGGS
                     EAGLDASETYTLKFTGAPHAKQGYHVKLTINAPGSQGKDVKHKVFWVEGCEQPQPPTE
                     VTPAAPTSHDVCGTANDTYTVPSTEGVDYLVDGKVVKAGTYPGSGSVTVTAKAQDGYK
                     LKGDSSWTFQFTDEPCQQPPTEVTPAAPSSTTPTCDNPNETVTPGDMPGVVWSPSGPT
                     TLKPGESVTYTASPAEGYEFPQGAQTEWTFTNNFDTSKCETPPPPPTSSHDFTVSPNQ
                     GSCTVMVPQAKVVFDFPASLGYQVRGSDETHWVYTEVQAGILPVTLPAVQPGQSVVYR
                     VVLSPLDDSVGLKHSDWFTFSVPTDCGNQPHHPNTPDTPDQPDQPSTPPTLTTAPHAG
                     MGGPTNDGGYFWLYGLVALMAAGGFVLTLRRRGGVR"
     gene            1682..2302
                     /locus_tag="VFG56_02820"
     CDS             1682..2302
                     /locus_tag="VFG56_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET6622841.1"
                     /translation="MALALNAWVSSRDEAQAISESGGGLGLPSLTPTPSGSATAVPSP
                     STNTPTVKATPKPSEPLAKPARPIRLLIPAIDVKAKVQRLGTLTDGSQQLPDNFTDVS
                     WWKDGQMPGQAGNAVFAGHTYSKGAGVFDNLPRLAEGNRIIVVTTKGRQTYRVVSVGS
                     VPRSDYASVADQITRRTGKSGVVLQTCGDWDGSEYHATSVVYATLT"
     gene            complement(2422..3501)
                     /gene="ychF"
                     /locus_tag="VFG56_02825"
     CDS             complement(2422..3501)
                     /gene="ychF"
                     /locus_tag="VFG56_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET6622842.1"
                     /translation="MSLSIGIVGLPNVGKSTLFNALTDAGALAANYPFATIEPNTGIV
                     PVPDARLAVLADMYNSAKVIPASVSFVDIAGLVAGASKGEGLGNQFLANIRECDAVVH
                     VVRAFSDTNIVHVDESHTPVSDIEVINTELALADLQTLENRRSTLEKQAKSNPKLRAD
                     LEQIDEIKHILSHGQAPSSDLIKRLTSNVQSLNLLTTKPVIYAFNVDEATLADDAKKT
                     ELKQLVAPHDVVFICAKLEEELAGLTPEDAKELLGSFGIEQAGLDQLITAAYHTLGLQ
                     SYLTAGPKEVRAWTITQGSTAPQAAGVIHGDFEKGFIAAQVVDYNDLVTAGSEAAAKA
                     AGKLRTEGKDYRMRPGDIVEFRFNV"
     gene            3598..4215
                     /locus_tag="VFG56_02830"
     CDS             3598..4215
                     /locus_tag="VFG56_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622843.1"
                     /translation="MPDKQPDPETTNSAVITGGEQRTPSHRRRTILIVLAIVVLAAVA
                     GYTAYRFYDNYRDQQAAETELKQKAEALGDSYDYTGFTPEQVAIKLQAVGLDWEAARH
                     GNLTDLSFEQAYAVARALRTDSNYERSLEAYKVAAAELSKEADYTFYRGYSEVALFAD
                     KIDLWKQVMQEQKQAILADDSLDKDTKQIVTAEIDEAIRLKELGW"
     gene            4209..6953
                     /locus_tag="VFG56_02835"
     CDS             4209..6953
                     /locus_tag="VFG56_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622844.1"
                     /translation="MVATLHGLRKNPLLAVLVAILIISWAVILSAPRADALSSKYFSD
                     DAGGDNCDAWITPGRIGCGRIEIRLIDGQTRYVRRTSTNLAAYFTKSSGSLRIYNANF
                     CNGVSADDVEGEDYFGNHTMRNGTAVTKFTFRTAGGTDVRYGRKYSGGGNCNDSINMT
                     FRNLRKDKRTGLYYVKIEVDTVDNRRGSYDGIQNSYSIAETGGGTYMIAQKEGYGSGY
                     GVTVQQRGTNPRNMTYKVRFGASCSVKNPGTYRLYYYDMDNDGGSGAQLGGKITMRLI
                     EVGTGRVTSWTPRGKANAPDFKRVTIRPGKRYVWEINNVYYNNTLQFSTPFDGIYYEK
                     PCDPKPDVCPNIPGNQASVPRGYIKQGGKCLKDHCPDIPGLQTNDSLCKVDVIPLAYI
                     SPAYVEVGDSATVTVSYKDTANYDKEQAKNDKKEEKSYVDIPSPYCKSSCHIQTKFYE
                     YSGIKYEGQIWFDNGDKKYRAADDDLVYDTDEFKGERFTGSGGTLGTHTTGETELSPL
                     DDPNFLVCSQSWLVSGYYTYRDRGDWEKVETPVYKYRYVYVGSGGNYRRDVSYRNGKR
                     IVRYVYNDSGPYSSSGNYIGYGGSYNSNGTYNPNDGAYDRDRYIDHYDYSYRFPDDDT
                     DKVRISPSSDPSTPAACSVIQRWPDIQVTGGDVRAGGSFADALDNCSASPARIIGHLH
                     SVESDLRGSFGEYGVLATDDISHYGSNSVVSTNGSLASELYFANTGAPGNFSGGWCFN
                     EVFDSFPNPTWTGNSWSGGNIELGPNDRMVIRVNGPARITGDILTSSIKINSINQLPE
                     FVLIADGDITIDSDVTEIDGIFVTKGNFITCNGHTQVNSSISLDEGSACREALVVNGA
                     VIAGGTIKAYRTNAASDDYLAPAELFNLDGATIISDYSAKLRTGEYTIIGERELPPRF
                     "
     gene            7006..8052
                     /gene="pilM"
                     /locus_tag="VFG56_02840"
     CDS             7006..8052
                     /gene="pilM"
                     /locus_tag="VFG56_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET6622845.1"
                     /translation="MKIFPAVGDYFALDIGTTAIRAIQLSGSHNAWTLERYGIAPVDL
                     RVSTSDAAEDQRKLAEIITTVIGQSGIRSRNVILGIPSNKMFATVIDMPDMPKEELAN
                     TIKYQAEQYIPMSIDEAKIDWTVIGKSINDPTKNEILLASVANSFSESRLDLVEGLGL
                     NVLAIEPDSLGLVRSLLPAGVADGRLIVEIGDFATDIVMTCGDNPRLIRSIPTGFQTL
                     VKAATQNLNVQPNQAQQFIMKFGLQPDKLEGQVLRAMEATLEQFVSEIDKSVKFFKTR
                     YPSVNVSGMIVSSYGVTIPGLSHYLGEKVGLTAEIGNPWQRVRVSTADQTKLQPMSAQ
                     FAVAIGLAQRGASS"
     gene            8049..8774
                     /locus_tag="VFG56_02845"
     CDS             8049..8774
                     /locus_tag="VFG56_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622846.1"
                     /translation="MITLNLLPDVKKEFLKAQRAKATWTSVSILVTIAAIGLTVLVAG
                     WVYGAQNVHKSVLTNSIKENEQKLKDKKIDKYLTIQNQLATISDLHNQKNDFSRLFGF
                     LPNLNPAVPDSIGLSSLTIDTEDETIDFQGDAPNYEALTTFKDTLLNAKVTYREPGSD
                     KPIEAVPLFSNVVVINSALIDNSKGSGTVVSFEIKVNYETAAFVYANKDVQVEVPNKD
                     TTNSTINAPVFSGEQAKPDEGTE"
     gene            8774..9499
                     /locus_tag="VFG56_02850"
     CDS             8774..9499
                     /locus_tag="VFG56_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622847.1"
                     /translation="MDKTKESAPSTDDNKQQLTGLKKRQQIAQANKMIFLWTAAAAVA
                     VSFCAVALQFLIREGLFNQRIISAKAETNQTLERNLTKADELKNNVQALLANTDLAAV
                     STENTDNNLEVVLDALPTDGDVTSFATSLQNIILVHSRADVTALSTKSQFDDDGSSSV
                     ESSSTANDDTDALPLPFTAEISGNYQQIHDTLVDVSRTIRPINITSLTVKGRDVNLDV
                     IISGDTYYLPAATVSLGSKEIEP"
     gene            9496..9738
                     /locus_tag="VFG56_02855"
     CDS             9496..9738
                     /locus_tag="VFG56_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622848.1"
                     /translation="MKKTDIAILILIVGVSLLVAFLLGKALFGGRVADPVQVETAEAI
                     SAEVTQPDKSIFNEHAINPSVEIQIGQSSNEQPFGN"
     gene            9751..11541
                     /locus_tag="VFG56_02860"
     CDS             9751..11541
                     /locus_tag="VFG56_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET6622849.1"
                     /translation="MAVLTEENQKVLEGWLVERGIIDQAKLDDYRTKAKADGQPVISR
                     LVDDKLVSNEKLTELTAKISGVPYVNLLKATVDPKVLTLLPKEVAERYMAVPLGELDK
                     RLAVAMLDANNIQAVDYLSNKIERPLKVYMASEEGIQQVLNQYRTDLARGVSEAIASS
                     QQTEAAPKGKKKAKEEDDDALVISQDSPISRALNTILEYAVRSSASDIHIEPSETELK
                     IRCRIDGVLREVMSLPKSIEPALISRIKILSNLKIDEHRIPQDGQFTVRTAGKDVDLR
                     IAISPVVWGEQVVIRLLDKTGSSFNLEEMGYAGRALRTIRKGIHKPNGMILTSGPTGS
                     GKSTSLYALIQEIKDDTVNIVTLEDPVEYKMGGVNQIQVNSEVGLTFASGLRSILRQD
                     PDVVMVGEIRDKETANLAVQAALTGHLVFSTLHTNSAAGVLPRLLDMGIEPFLIASTV
                     NTVIGQRLVRRNAENKEVYKSSDLETKSIQDTVGYLLPKTKQEATKMAEDMGYKDLPL
                     GGQSSYTLAKGKDSPKSPGGYKGRVGLYEVMDISEEIQNLIVKEATSAQIQRQAVAEG
                     MVPMRQDGYLKALQGLTTLDEVNRVAANMA"
     gene            11563..12633
                     /locus_tag="VFG56_02865"
     CDS             11563..12633
                     /locus_tag="VFG56_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET6622850.1"
                     /translation="MATSNLRIETLLEDVIKKKASDLHLQVGLPPMLRIDGALNPIAG
                     LEVLNEEEVERLIFAILDQDQQQILLKDKEFDFSFAFGDLGRFRVNAFHERGNLAAAL
                     RLIPNEIKTITELGMPTIVSDFASYPRGLVLVTGPTGSGKSTTLAALVDKINSEKSAH
                     IITIEDPIEFTHKSKKSVVVQREVHYDTYSFSAALRSSLRQDPDVVLIGEMRDLETIS
                     AAITIAETGHLVFATLHTNSAAQSIDRMIDVFPPHQQPQIRAQLANILMAICSQRLVP
                     SIGGGRAVAAEILTATSAVRNIIREGKSHQLDAVIQTGGDKGMQSMDKTLAGLVQAGT
                     VTYEEARNFAVDITEFDRMMRG"
     gene            12641..>12857
                     /locus_tag="VFG56_02870"
     CDS             12641..>12857
                     /locus_tag="VFG56_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6622851.1"
                     /translation="MLTFSYSARDQSTNKIVKSTVQAESERAAAKLLLGRNLTPLKID
                     VEGQNKNFLDALSNHVSTKDRVIFTRQL"
BASE COUNT         3236 a         3309 c         3450 g         2862 t
ORIGIN      
        1 ccggccccgg ctgatgacga caacgacggc gtgccgaaca ctcaggacga gtgtgatggt
       61 actgccgccg gtgccgatgt cgacgctaac gggtgttccg ctgaccagcg ggattccgat
      121 ggcgacggtg tctccgatgc cgatgacaac tgcgccgaca ctccgtctgg taccgaggtc
      181 gatgaccacg gttgtccagt cgatggtggg ggtgacgacg gtggcaatgg cgggcataac
      241 ccgcccggca acaacggcac catcaagatc gatgacgaag ccttcgatga ccatccgaac
      301 aacgagccgc acgtcggctg cacgttccag gtagacttct acggctacga caagggtgac
      361 tactacgctg atgtgaactt cgagctgcaa tctccgacca agaacggtcg aacgcttgag
      421 gtgaccagtg gcgatctgga ccccttcatc ggtgaagacg atgccagtgg aggtggcagc
      481 gaggccggtc tggacgcgag tgagacctac acgctcaagt tcaccggcgc gccgcacgcc
      541 aagcagggat accacgtcaa gctgacgatc aacgctcctg gttcccaggg caaggacgtc
      601 aagcacaagg tgttctgggt tgaaggctgt gagcagcctc agccgccgac cgaagtcacg
      661 ccggccgcgc cgacgtctca cgacgtctgc ggtaccgcca acgacaccta caccgtgccc
      721 agtacggagg gtgtggatta cttggtggac ggcaaggtcg tcaaggctgg tacctatccg
      781 ggctccggct cggtgacggt taccgccaag gcgcaggatg gctacaagct gaagggtgac
      841 tcctcctgga cgttccagtt cacggatgag ccctgccagc agccgccgac cgaagtcacg
      901 ccggccgcgc ccagcagcac gacgccgacc tgcgacaacc cgaacgagac ggtcacgccc
      961 ggcgacatgc ctggcgtggt ctggagcccg agcggtccta cgactctcaa gcctggtgaa
     1021 tcggtgacct acaccgccag cccggccgag gggtacgagt tcccgcaagg tgcacagacg
     1081 gagtggactt tcacgaacaa cttcgacacg tcgaagtgtg agactccgcc tccgccgcca
     1141 acttcgagcc atgacttcac ggtgagcccg aatcagggca gctgcacggt catggtcccg
     1201 caggccaagg tggtgttcga tttcccggct tcgctgggtt atcaggtgcg gggcagcgat
     1261 gagacccatt gggtctacac cgaggtccag gccggtatcc tgccagtgac actgccggcg
     1321 gtccagccgg gtcaatcggt ggtctatcgg gtggtgctgt caccgctcga tgactcggtg
     1381 gggctgaagc actccgactg gttcacgttc agtgtgccga cggactgtgg caaccagcca
     1441 catcatccga acacgcctga cacaccagat cagcccgatc agccgagcac gccgccgacg
     1501 ctgactaccg cgcctcatgc aggcatgggt ggtccgacca acgacggtgg ctacttctgg
     1561 ctgtacggcc tggtggcgct gatggcggcc ggtggcttcg ttctgacgct ccggcgtcgg
     1621 ggcggtgtcc gttagccgga agttcgttct actgatattc gcgatcgttt tcgcggtgtc
     1681 agtggcgctc gctctgaacg cctgggtatc gtcgcgagat gaggcccagg cgatcagcga
     1741 gtcgggcgga gggctcggac taccaagtct gactccgacg ccatcgggat cagcaacggc
     1801 agttccgtcg ccctcgacga acacccctac ggtcaaagca acgcctaagc catctgagcc
     1861 attagctaaa ccagcccggc caattcgctt gttgatcccc gcgatcgacg tcaaggcgaa
     1921 ggtgcaaaga ctggggacgc taacggatgg ctctcagcag ctacctgaca atttcactga
     1981 cgttagttgg tggaaagacg ggcagatgcc ggggcaggca ggcaacgcgg tattcgccgg
     2041 tcacacctat tccaagggtg ctggcgtctt cgataacttg cccaggctgg cagaaggaaa
     2101 ccgcatcatc gtagtcacga ccaagggccg gcagacctac cgggtagtgt cggttggcag
     2161 cgtgccacgc tctgattacg cgagcgtagc cgatcagatc acccgtcgaa cggggaagtc
     2221 tggtgtagta ctgcaaactt gcggtgactg ggatggctcc gagtaccacg ctacgagtgt
     2281 ggtatacgcc acgctcacct gagtagaacc gaatcgggtt cgggcacgcg aacagggaca
     2341 acctgtctca cgcgtgcccg gcccgaatca acttttagca atttagttgt tagcaaatag
     2401 caattactaa aaactaagcg gctaaacgtt gaacctaaat tcgactatgt cacccggacg
     2461 catacggtag tctttaccct ctgttcgtaa cttaccagcg gccttagctg ccgcttccga
     2521 accggcagtc accagatcgt tgtagtcgac gacttgagcg gcgatgaagc ctttttcgaa
     2581 gtcgccatgg atgacaccag cagcctgggg cgcggtcgaa ccttgggtta tagtccaggc
     2641 tcggacttct ttcggcccag ctgtcagata gctctggagt ccgagagtgt gataggcggc
     2701 ggtaatcagt tgatcgagtc cggcctgctc gataccaaag ctacccaata actccttggc
     2761 atcttccggt gtcagaccgg ccagttcttc ttccagtttg gcgcagatga aaacgacgtc
     2821 gtgtggtgcg accaattgtt tgagttcggt tttcttggcg tcgtcagcta aggtggcctc
     2881 atcgacattg aaggcataga tgaccggctt ggtggtcagc agatttagag actggacatt
     2941 ggaagttagg cgtttgatga ggtcggatga tggggcctga ccgtggctta agatgtgttt
     3001 gatctcatcg atttgttcta ggtcggctct taacttcgga ttagacttgg cttgtttttc
     3061 cagtgtcgag cgtctatttt ctagcgtctg caaatcggcc agagccagct cggtattgat
     3121 aacctcgata tcactaacag gggtgtgact ttcatctaca tgaacaatat ttgtgtccga
     3181 gaaggctcgc acgacatgaa ctacagcgtc acactcacga atattggcca agaactgatt
     3241 gccgagtcct tcgcccttag atgcgccggc gaccagtccg gctatatcga cgaagctaac
     3301 actggccggg atgaccttgg cggagttgta catgtcggct agaacagcca gccgagcgtc
     3361 agggaccggc actataccgg tgttgggctc aatggtggca aaaggatagt tagcagctag
     3421 cgcgccagcg tcagtcaggg cgttgaacag agtagatttg ccgacgttag gtaggccgac
     3481 tataccgatt gataaactca ttgagattat tttaacagag gtaatggtct gataccaaat
     3541 aagctttaac cgattgaact aatctgctat gcttatggtt aaaagcgagt ataagccatg
     3601 ccagataaac aaccagatcc tgaaacaact aattcggcag tcatcactgg cggcgaacaa
     3661 agaacgccca gccacaggcg tcggacaatt ctaatcgtgc tagccatagt ggtattggcg
     3721 gcagttgccg gctacaccgc ttataggttt tatgataatt accgtgatca acaggcggca
     3781 gagacagagc ttaagcaaaa ggccgaggct ttgggtgata gctatgacta tactggtttt
     3841 actccagagc aagttgcaat taagcttcaa gcagtaggct tggactggga ggcggcacgc
     3901 catggcaact tgaccgattt gagttttgag caggcctatg ctgtcgccag agccttgagg
     3961 accgacagta attatgagcg ctcactggag gcctacaagg tagccgcagc tgaactgtcc
     4021 aaggaggctg actacacgtt ttatagaggc tacagtgagg tggctctgtt tgccgataag
     4081 atagaccttt ggaagcaagt tatgcaagaa caaaagcagg caattctagc tgacgacagc
     4141 ctggataagg atactaaaca gatcgtaacg gccgaaatcg acgaggctat tcgcctgaag
     4201 gagctggggt ggtagctacg ctacatggtc tacgtaaaaa cccgctacta gctgtattag
     4261 tggcgatttt gattatcagc tgggcggtta tcttatcggc tccgcgagct gacgccctta
     4321 gctccaagta tttttctgat gatgccggtg gcgataactg tgacgcttgg ataacgccag
     4381 gccgaatcgg ctgtggccgg atcgagatcc gcttaatcga cggtcagacc cgctacgtaa
     4441 ggaggacatc taccaatttg gcggcttact tcaccaagag tagtggaagc ttgaggattt
     4501 acaacgctaa cttttgtaac ggtgtcagtg cggatgatgt agagggtgaa gattactttg
     4561 gtaatcatac gatgagaaac ggtaccgcgg taactaaatt tactttccgt acagcaggcg
     4621 gtaccgatgt cagatatggt cgcaaatata gtggtggtgg aaattgtaac gattctatca
     4681 atatgacttt tagaaactta agaaaagaca agcgaacagg tctttactat gtcaaaatcg
     4741 aagtagatac tgtagacaat cgtcgaggca gctatgatgg tatccagaac tcttattcca
     4801 ttgctgagac cggcggaggt acctatatga tcgctcagaa agaaggctat ggtagcggtt
     4861 atggcgtaac cgtacaacag cgtggtacca atcctaggaa catgacctac aaagtccgtt
     4921 tcggcgccag ttgcagcgtt aaaaacccgg gcacttatcg tttgtactac tacgacatgg
     4981 ataacgatgg tggttccggt gcccagctag gtggcaagat tacgatgcgc ctgatcgaag
     5041 ttgggaccgg tcgtgtaact agttggacac ctcgcggaaa ggctaatgcg cccgacttca
     5101 agagagtgac tatacgtcca ggtaagcgtt atgtctggga gatcaataac gtttactata
     5161 acaacaccct gcaattctca acaccgtttg acggcattta ttacgagaaa ccgtgtgacc
     5221 ctaagcctga cgtctgcccc aacatacccg gtaatcaggc tagcgtgcca agaggttata
     5281 tcaaacaagg cggcaagtgt ctgaaagacc actgccctga tatacccggt ctacagacca
     5341 atgattcgct atgtaaagtc gatgtgatac cgttggccta catatcaccg gcctatgtag
     5401 aggttggtga ctcggccacg gttaccgtga gctacaaaga taccgccaat tacgacaaag
     5461 aacaggccaa aaacgacaaa aaagaagaga agagttacgt tgatatacct tcgccttact
     5521 gtaagtcaag ctgccacatc caaaccaagt tctacgagta cagcggtatc aagtacgaag
     5581 gccagatctg gtttgacaac ggtgataaaa aataccgggc agccgacgat gatttggtct
     5641 atgatacgga cgagtttaag ggagagaggt ttaccggatc cggtggaact ttaggtaccc
     5701 acaccactgg tgagactgaa ttgtcaccgc ttgatgaccc gaactttttg gtttgctccc
     5761 agtcatggtt ggttagcggt tactatacgt accgtgacag aggtgactgg gagaaggtgg
     5821 agacgccagt gtataaatat cgctatgtat atgtcggttc gggcggtaac taccgccgcg
     5881 acgtttccta caggaacgga aaacgcatag tccgttatgt ctataatgac tctggtcctt
     5941 acagctcaag cggtaactac ataggttacg gcggtagcta caatagcaac ggtacttaca
     6001 atcctaatga cggcgcgtat gaccgggatc gctacatcga tcactacgac tatagctaca
     6061 gattcccgga tgatgacacc gataaggtac ggatatcacc gagcagtgat ccttccacgc
     6121 ctgcggcctg ttcggttatc cagcgttggc ctgatattca agtgactggc ggtgacgtca
     6181 gggccggcgg tagcttcgcc gatgctctag acaactgtag tgcatccccg gccagaatca
     6241 tcggtcactt gcattcggtg gaatccgatt tgcgtggttc attcggcgag tatggtgtgc
     6301 tggcaaccga tgacatcagc cactatggct ccaacagcgt agttagtacc aatggcagcc
     6361 tagccagcga gctatacttt gctaacaccg gagcgcctgg taatttcagt ggcggctggt
     6421 gtttcaacga ggtcttcgat agtttcccga atcctacctg gactggcaac tcctggtcag
     6481 gtggcaatat cgagttgggg ccgaatgaca gaatggtcat ccgggttaat gggccggctc
     6541 gtatcaccgg agacatccta acctccagta tcaagataaa ttcgatcaat caactgccgg
     6601 aattcgtatt gatcgccgac ggcgacataa ccatcgacag tgatgtaacg gagatcgacg
     6661 gtatatttgt tacaaagggt aacttcataa catgtaacgg ccacactcaa gtcaacagct
     6721 ctattagctt agacgagggt tcggcttgtc gggaggcttt ggtagtcaac ggtgcggtta
     6781 tcgctggcgg taccatcaag gcttatcgaa ctaacgccgc aagtgatgac tacttggcgc
     6841 cagcggaact attcaatctg gacggcgcta ctatcatctc cgattactcc gccaagctcc
     6901 gaaccggtga atacacgatt atcggtgagc gtgaactacc accgcgattt tagcttaagc
     6961 aagttgttta tttatcatag tgtttatgta taataagcag tagttatgaa aatatttcca
     7021 gcggtgggcg attacttcgc acttgatatt ggtacaacgg cgattcgggc tattcaactg
     7081 tccggtagcc acaacgcttg gactctagag cgttacggta tagcaccggt tgatcttagg
     7141 gtgtcgacat ctgatgcagc cgaagaccag cgcaaactag ctgaaatcat caccacggtt
     7201 atcggtcaga gcggcatccg ttcacgtaat gtcatactcg gcatcccgag taacaaaatg
     7261 ttcgctacgg tgattgacat gccggatatg cccaaagaag aattagccaa caccatcaag
     7321 taccaggctg agcagtatat cccgatgagt atcgacgaag ctaagatcga ctggactgtt
     7381 atcggtaaat ccatcaacga cccgacaaag aacgagattt tactggcgtc ggtagctaac
     7441 agcttctccg aatcgcgcct agacttggtc gagggacttg gcctgaacgt cctggctatc
     7501 gagccggatt cgctcgggct ggtccgatcg cttttgccgg ctggcgtggc tgatggtcgc
     7561 ctgatcgtcg aaataggtga cttcgccacc gacatcgtca tgacctgcgg tgacaaccca
     7621 cgattaatcc gctcgattcc gactggtttc caaaccctag ttaaggcagc tactcaaaat
     7681 ctcaacgttc agcccaacca ggctcagcaa tttatcatga agtttggctt gcagccagac
     7741 aagttagaag gccaggtcct aagggctatg gaagctacgc tagaacaatt tgtcagtgag
     7801 atcgacaaat cggttaagtt ctttaaaacc cgctatccga gcgtcaatgt cagcggcatg
     7861 atcgtatcta gttacggcgt aaccataccc ggcttgtcgc actatctagg tgaaaaggtc
     7921 ggtctgacag ccgagatcgg caatccttgg caacgcgttc gtgtgtcgac agccgatcag
     7981 actaagctac aaccgatgtc ggcccagttc gccgtagcta tcggactagc tcagcgaggc
     8041 gcttcgtcat gatcacgttg aacttactgc cagatgtcaa aaaagaattc ttgaaggctc
     8101 agcgcgccaa agcgacttgg actagtgtat ctatcctagt gacgattgcg gcgatcggtt
     8161 tgaccgtatt ggtggcaggc tgggtctatg gagctcagaa cgtccacaag tctgtcttga
     8221 ctaattcgat caaggaaaat gagcaaaaac taaaagacaa aaagatcgat aaatacctga
     8281 ctatccaaaa ccaacttgct accatatcag acttgcataa ccagaagaat gacttctctc
     8341 gactgttcgg tttcttgcca aacctcaacc cggctgtacc tgacagtatc ggcctgtcga
     8401 gcctgacaat cgataccgaa gatgagacta tcgacttcca aggcgatgcg ccaaactatg
     8461 aagcccttac tacatttaag gacaccctgc ttaacgccaa ggtgacttac cgtgagcctg
     8521 gctctgataa gccgatcgaa gccgtcccgt tgttttccaa cgtggtagtt atcaatagcg
     8581 cccttataga caacagcaag ggtagtggca cagtggtgtc gtttgagata aaagtcaact
     8641 acgagacggc cgcctttgtt tatgccaata aggacgttca agttgaggtg cctaacaagg
     8701 acacgacaaa ctccactatc aacgcaccgg tattttcagg agagcaggct aaaccggatg
     8761 aagggaccga ataatggata agaccaaaga atcagcgcca tcaaccgacg acaataagca
     8821 gcaattgacc gggcttaaaa agcgccaaca gatcgctcag gccaataaga tgatctttct
     8881 ctggacagcg gccgcagccg tggccgtgtc attctgtgca gtagccttgc agttcttgat
     8941 ccgggaaggc ttgtttaacc aacggatcat cagcgctaaa gctgagacca accagaccct
     9001 ggaacgaaac ctaaccaagg ccgatgagct caagaacaac gtacaggcgt tgctggcaaa
     9061 taccgatctt gcggctgtta gtactgaaaa caccgacaat aacctcgagg tagtcctcga
     9121 cgctttgcct acagatggcg acgtgaccag cttcgccact tcacttcaga acattatcct
     9181 ggttcactca cgcgccgatg tgaccgctct atcgaccaag agtcagttcg atgacgatgg
     9241 cagctctagc gtcgagagct cgagtactgc caatgatgac accgacgctt tgccgctgcc
     9301 gtttacggcc gagatcagtg gtaattacca gcagattcac gacaccttag tagatgtatc
     9361 caggactatt cgtccgatta acattacttc cttaaccgta aaaggacgcg atgtcaatct
     9421 ggatgtgatt attagcggcg acacctacta cttgccggcc gccaccgtaa gtttaggttc
     9481 caaggagatt gaaccgtgaa aaagaccgat atagctatcc taatcttgat cgtcggcgtc
     9541 agcttgctgg tagccttctt gctgggcaag gctttgtttg gcgggcgggt ggccgacccg
     9601 gtacaagtcg agacggctga agcgatttcg gctgaagtaa cccaaccgga taaatctatt
     9661 tttaacgagc atgcgattaa tccatcggtt gaaattcaga tcggtcaatc gagtaacgaa
     9721 caaccgttcg gtaattagga gtacgactca gtggcagttc taactgagga gaaccaaaaa
     9781 gttctagagg gctggctggt tgagcgcggt atcatcgatc aggcaaaact tgatgattat
     9841 cgaaccaagg ccaaagctga tggccaacca gtcatcagtc gcctagtcga cgacaagctg
     9901 gtcagcaatg agaaactaac cgagctaact gccaagatca gtggcgtacc ttatgtcaat
     9961 ctcttaaagg ctaccgtcga tcccaaggtc ttgacgctct tgcctaaaga ggtggctgag
    10021 cgctatatgg cagtgccatt aggcgagctt gataaacgcc tagccgtagc tatgctggac
    10081 gctaacaata tccaggccgt tgactacctg agtaacaaaa tcgaacgacc actcaaggtc
    10141 tacatggcct cagaagaggg cattcagcaa gtactgaatc aataccgaac cgatttggcc
    10201 cgaggcgtca gcgaagctat tgcctcctct caacagactg aagcggcgcc aaagggaaag
    10261 aagaaggcta aagaagagga tgatgatgct ctagtcatca gccaagattc gccgatttcc
    10321 cgagcactta ataccatttt ggaatacgcc gttcgttcta gcgcttccga catccacatc
    10381 gaaccatctg agaccgagct gaagattcgc tgtcgaatcg acggcgtact gagagaggtc
    10441 atgagcttac ctaaatcgat cgagccggcc ttgatctcac gtatcaagat cctgtccaat
    10501 ctcaagatcg acgagcaccg catcccgcag gacggccagt ttaccgtgag gactgccggt
    10561 aaggacgtgg acctgcgtat cgccatcagc ccggtagtat ggggcgaaca ggtagttatt
    10621 cgtttgctcg ataagaccgg ttctagcttc aatcttgagg agatgggtta tgctggccgg
    10681 gcgcttcgga ctatccgaaa gggtatccat aagccaaacg gtatgatctt aacctccggt
    10741 cctaccggtt cgggtaagtc aaccagtctc tatgccctta tccaagagat caaagacgac
    10801 accgtcaata tcgttactct ggaggacccg gtcgaatata agatgggtgg cgttaaccag
    10861 attcaagtta actccgaagt cggcctgacg tttgcctcgg gtcttcgctc tatcctgcga
    10921 caagaccccg acgtggtgat ggtaggtgag attcgtgaca aagagacggc taacttagcc
    10981 gtccaagccg ccttgaccgg tcacttggtt ttctcgacac ttcacaccaa ttcggctgct
    11041 ggtgtgctgc cacggttgct cgacatgggt atcgagccat tcttgatcgc ttcaacggtc
    11101 aataccgtca tcggtcagcg gctggttcgg cgcaacgccg agaacaaaga agtgtataag
    11161 tcgtctgacc tggagactaa gtctatccaa gacaccgtag gctatctgct gccaaaaacc
    11221 aagcaggaag ctaccaagat ggccgaggac atgggctaca aagaccttcc cttgggcggc
    11281 caaagctctt atactttagc taaaggcaag gatagcccta agagcccggg tggttacaag
    11341 ggacgagtcg gcttgtacga agtcatggac ataagcgagg aaatccaaaa cctgatcgtc
    11401 aaagaagcta ccagtgctca gattcagcga caagctgtgg ccgagggtat ggtgccgatg
    11461 cggcaggatg gctacctcaa ggcgctacag ggcctgacaa cattagatga agttaaccgc
    11521 gtagcggcca acatggcata aaacgggagg gaagaaagaa aaatggcaac cagtaactta
    11581 agaatcgaaa cgctgctaga agacgttatc aaaaagaaag cgtcggacct ccacttacag
    11641 gtaggtttgc cgccgatgct caggatcgat ggtgcactga atccgatcgc cggattggaa
    11701 gttcttaacg aagaagaagt cgagcggctg atttttgcta tcttggatca agatcagcag
    11761 cagattttgc tcaaggacaa agaattcgac ttttcctttg ctttcggcga cctcggtcgt
    11821 tttcgggtca atgcctttca tgaacgcggc aacctggcag cggctctccg gttgattccc
    11881 aatgagatca agactatcac cgagcttggt atgccgacca tcgtcagtga cttcgcctct
    11941 tatccgcgcg gcctggtttt ggtaactggt ccgactggct ctggtaagtc gactacacta
    12001 gccgctctgg tcgacaagat caactccgaa aagtcagccc acatcatcac catcgaagat
    12061 ccgatcgagt ttactcacaa gtcgaaaaag tcggtcgtgg tccagcgaga ggtccactac
    12121 gatacctaca gtttctcggc cgcgcttagg tcgtctttac gtcaagatcc tgacgtagtc
    12181 ctgatcggtg agatgcgcga cttggagacc atatcggccg ccataaccat cgccgagacc
    12241 ggccacttgg tctttgcgac actgcacact aactcggctg cccagagtat cgaccgtatg
    12301 atagacgtat tcccaccgca ccagcagccg caaatccgag cccagcttgc caacatcctg
    12361 atggctattt gctcacagcg cttagtccca tcgatcggtg gtggtcgagc ggttgccgcc
    12421 gaaatcctaa ccgcaacttc agccgtccgc aatatcatcc gtgaaggtaa gtcccaccag
    12481 cttgatgctg tcatccaaac cggtggagac aagggcatgc agtcgatgga caagacgctg
    12541 gcgggattgg ttcaggctgg tacggtaact tatgaagagg cccgtaactt tgccgtcgat
    12601 ataaccgaat tcgacaggat gatgaggggg tagattttag atgctgactt tttcttacag
    12661 cgcgcgcgat cagtcgacta acaagatcgt taagtcgacg gttcaggcgg aaagcgaacg
    12721 agctgccgcc aaactattac ttggccggaa cttaacgccg cttaagattg atgtcgaagg
    12781 tcagaacaag aacttcctcg atgccctgtc taaccacgtc agcactaaag accgagttat
    12841 attcacgcgc cagctgg
//
LOCUS       DASSBW010000098        13664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1407091, whole genome shotgun sequence.
ACCESSION   DASSBW010000098 DASSBW010000000
VERSION     DASSBW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407091"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFG56_02875"
     CDS             <1..695
                     /locus_tag="VFG56_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622852.1"
                     /translation="SGNLFSVAAPSFGLRPEPLFGLPLLILIVLGILQIFRDNFSARA
                     YVLIGWLAVFFVILSINADHVLDLFVPAVLLLAVGVETLLHEWYKLFPRNPYARIGAV
                     IPLTILILGISISEVARYFDGYRYGVDYSNTNYSLALKPLRNQLNQSSNQSTYLIAPT
                     DQLDFYKLLANRFDNLTVSDDYVPASSSQLVIVLHGTQLEAAYPVDQIKHIETTPLRH
                     QPLLFSVYQTNQ"
     gene            734..1021
                     /locus_tag="VFG56_02880"
     CDS             734..1021
                     /locus_tag="VFG56_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET6622853.1"
                     /translation="MLDQAKAVMQMKKMQKELAREIVEVEAGDGAVVVQVTGEMKLKK
                     VNIDPESVDLDDIEQLEKWVEIAMRDAYAKAQELAAEKMKPLMGSLGNFGL"
     gene            1046..1663
                     /gene="recR"
                     /locus_tag="VFG56_02885"
     CDS             1046..1663
                     /gene="recR"
                     /locus_tag="VFG56_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET6622854.1"
                     /translation="MATYQLPKALTDLIDALGVLPGVGPRTAERFASYLLKADDNLSQ
                     KLIGALGSLKSGVKTCPMTFALMDASETISPLYEDKARNKRLVAVVEDPLDIVALERT
                     DQFDGTYHVLGGVISPIDNVGPEKLHIPELVKRLEQDKVEEVILATNASVEGESTALY
                     IQRQIDEAGLKPVISRLARGIPAGIDLEYADQITLSHALKGRQVL"
     gene            1660..2634
                     /locus_tag="VFG56_02890"
     CDS             1660..2634
                     /locus_tag="VFG56_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET6622855.1"
                     /translation="MSKLVDFIDRHDSFVVIQAENPDGDSLGSALGLEAIIAETGKSV
                     QLYCPVEIPKYLRYVNGWDRVEADFPTQVAAAIIVDTSSSTLLSKTLDDPVAKHFLTD
                     QPVLVVDHHAVVGGELTFDHDQLIDTEVVATGELIAKEVLKSKLKLPEDAATALTIAI
                     LSDSLGLTTEATTAASYRTIADLVQHGAKPAEIESARREFMKKSADILAYKGRLIGRI
                     EYHLDGRLALVHIPWEEIEAYSDQYNPSVLVLDEMRLVEGVDVAVAIKTYPDGKLTGK
                     LRSNRPVSSQVAGFFGGGGHPYAAGFRIYESYDNMLPELVEATRKALS"
     gene            2631..4061
                     /gene="cysS"
                     /locus_tag="VFG56_02895"
     CDS             2631..4061
                     /gene="cysS"
                     /locus_tag="VFG56_02895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET6622856.1"
                     /translation="MSLQLYDTASRIKREFKPQVDNLVKLYTCGPTVYNELQIGNWLA
                     YLRWDLLVRVMTLSGYRIDRVMNITDVGHLVSDADDGEDKMEKGARREGVSAWEISKR
                     YTDSFVNGLAELNLIKPDHLAKATDFIAEQIDFIRGLEAKGHTYSIDDGVYFDTATFP
                     DYAKFARLDLDDQKAGARVVYNPDKRNPSDFALWKFSPGDKQRDMEWDSPWGKGFPGW
                     HIECSAIARHYLGDTLDIHTGGIDHIPVHHTNEIAQSVALTGQPLANYWLHADFLTSD
                     GTKISKSLGNGWTLQDLTDKGFRLVDLRLFALQSHYRSETNFTWDNLQAAANRLNNWL
                     SLAELRWQIHDTVVDDEDKAVSESSLNLRRAKHQTLQALQDDLNSPQALSLIDQQIDQ
                     IKAEIGNLQQDVLDDFIDFIENFSGLRLKQATPDIDDATKQLILERLRAREARDFQKA
                     DELRDQLVKTGIRMLDTTSSQLWTRY"
     gene            complement(4062..5180)
                     /locus_tag="VFG56_02900"
     CDS             complement(4062..5180)
                     /locus_tag="VFG56_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6622857.1"
                     /translation="MKVKIDIDTQTFVRLGFVTLAFVLGIYLFFHLTTPITIIVLALI
                     LAIALNPPVTRLARRLPGKSRVGATAIAYLIVVAILAAIIFLVVPPVIQQSSKFAATV
                     PGLIDQVTQNSRGFHQFLDEYGLQQTFNNSVDQLKGQVSALAANLGGLLVASVKSILA
                     GLVALLFILVIAFLMLIEGPTWMERFWALYKNKALLKRHKNVVYKMYKVVSGYVNGQL
                     IVAAIAAVCSLATILILSAVFDMDANIALPLATIVFISGLIPMFGATVGAILVALVLL
                     LNSVLAAIVFIIYFIIYQQIENNIISPSVQSKTVELSALIVLLAVLIGLQLFGLLGGI
                     ISIPIAGMIRVLVIDYLEHDHGRSEPKENSGWLKKIKL"
     gene            complement(5220..5372)
                     /locus_tag="VFG56_02905"
     CDS             complement(5220..5372)
                     /locus_tag="VFG56_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622858.1"
                     /translation="MTKKAKTQSQKHKKTPATINNFEPTKVALAVTCLGVSVVVLLAI
                     IAVTTL"
     gene            5435..7087
                     /locus_tag="VFG56_02910"
     CDS             5435..7087
                     /locus_tag="VFG56_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6622859.1"
                     /translation="MDLQKELKQIFRGEVSTDDALLTKYSHDASLFELRPQVVAAPLD
                     SADIKAVVKYVLEHKKSQPNLSITARSAGTDMSGAAINDSIVLSMTDHFNRIEELKAN
                     SARVQPGVYFRDFDKKCADLKVMLPSYPASRDLCTVGGMANNNSGGERSLEFGKTANF
                     VEELRVVLADGNEYTMRPLNKHELMKKIAQKDFEGALYGQVFDLLEKNYDLIKNAKPA
                     VSKDSTGYHLWDAWDRDTGIFDMTKIIVGAQGTLGLVTDIRFRLVPRKEFSGLLVGYL
                     KNQDQLGEIINTVLKHKPATFESFDDNTLWLSFKFFPAFMKKLGFWPWVKLAIQLIPD
                     GLALIKGVPKLILMVEFNGHSQEEVDAAIDNMKEDLDNYNFTYLEKDDTLAKSNKFWI
                     MRRESFNLLRSKVKDKHTAPFIDDLIVPPPELPVFLPKLRQIIKKYKLLATVAGHMGD
                     GNFHIIPLMKIEDPHERAKLQPAMKEVNALVLKHHGSISGEHNDGLVRGPWLETMYGA
                     EYFQVLKRTKRIFDPQNIFNPHKKTDANWDYSFSHIRQNFDQ"
     gene            complement(7178..9073)
                     /locus_tag="VFG56_02915"
     CDS             complement(7178..9073)
                     /locus_tag="VFG56_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET6622860.1"
                     /translation="MSRTIKIAIALSLAGIMAFTVILASLFGLGGVVSARQQMCQQID
                     SSGVGITVQAANVSLNSANLTVKGGPMQPAQAKNAAEIIGIGVNRRLPSRAWKVLLVA
                     STVESTLLNLPYGDSDSLGLFQMRPSVGPYYWGTPEQILDTNYAINRMYKELEKTVGW
                     QQMSIGDAAQAIEGSAFPERYALWVHEAEATVAAAVTNSQQSTGVSTVAADWTCQGPN
                     LRIATLNVRGSSHEAHGPERVRQLAGMVQLNNLDVVGLQELQSNQRQALVDELSGQYG
                     IYPSSPHNVHHSVENSIIWNQARLTKLDAGISTGYRYFNGSPLHIPWVKLKDNYSQTV
                     FIVQNTHDPADGRHGQSARWREANAHQHLADAKVWLKQGLPVFLTGDFNSTFDIRFGG
                     PTSDSYLIGHDKSRLPYCIMTASGDMLNAYDAMEGKTGCKTTDAQANADRQVKGRIID
                     HVYVSKGVDVVNWRWLETGSASDHDVLYIDVSFVSGGDGKWVLPLEPGSYTLTDPYRP
                     YPNGHNGLDFAGACGSPVQADSGGRIVRAGYDSGWGNFIVIQHAPRLWSLFGHMTGFA
                     PGIGVGQTVSTGQLIGYEGSTGNSTGCHVHVTIATDLDWAMQGRAVGGKGSVDPAAFY
                     RSKGLRP"
     gene            complement(9090..9674)
                     /locus_tag="VFG56_02920"
     CDS             complement(9090..9674)
                     /locus_tag="VFG56_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622861.1"
                     /translation="MKRFSKRTWLPQPSYVLFGLVIVGLVLAIVYGLARPGRTDADKP
                     PQQAVASASTSADPVATVEDKHESLSQLEARTRAFVQAFYDYNRSLTALSQRRAVRSL
                     ATPRFMRKTFFGLTDSQADQAIRSGKFQVHGSVQSIWPSEVYGQPPTVELPVVVTMTK
                     TDETGSTIDRYDQLVRMTWIKRPTKGWLLDSLTQ"
     gene            complement(9658..11121)
                     /locus_tag="VFG56_02925"
     CDS             complement(9658..11121)
                     /locus_tag="VFG56_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622862.1"
                     /translation="MAMGHPFFAQPHPLKRGIPLMVDMKSKELLLFDPWLLKLNGVIN
                     STFGLILGHKGDGKSSTMKIMASRLMLTSAGYQMMRIAINDYKPEGDAGEYEKLSRAY
                     RSKVFRINEMRVNVFDNRLFIQHDGSTNALALLEIAHILCETFLPNGANRLIGNDGVA
                     LRAAVYAMMSMDKNLWSPHSLLYKLLTLSDEDINGYYRSLDDKIKLQLEGKHDRLSVE
                     LQKTTRAQLDDLYRQDNNACLEDIKKSAVYVASLLDGVINGPYGYMFGDEHSLYDVMT
                     QRAVTKDWRNVPPDGETLMRTIDARIKLAAIEGNRLDLLPHLELDDEKHKSMGNLVYA
                     TQHSHLSEIGRGVHTCNLSASHYLHSIRRGAVGSELYNLGETIIDNLGFVMIGSQPNK
                     KQVLGELQDRYELSDADTRQLTRMPKYHFGVKLGQETPMIFGRVFATPSELPLLPTDA
                     AANRMQNKPSILDVDDMKRFAKENGIRYLGLDHETVQ"
     gene            complement(11336..12985)
                     /locus_tag="VFG56_02930"
     CDS             complement(11336..12985)
                     /locus_tag="VFG56_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622863.1"
                     /translation="MSNLEALPKFSIPQNKLQRRSWAGWTGVDIAFGVTSLLAAIALF
                     VYINDFLAMMAILFVLVPLNIRTEYGRLYWELATGLYGFFYIHLALRDVLWIDEDDSG
                     WLRRILREKRYRKNREKRQVIPVKLEMVQALGESFGVLHQTDRNLDHIFIVADGGRMS
                     SLDVMQQHNIVNELAAATNQVAAQSRIKIGVSYLRYGRPDDFNDLNDYLAESVNPVVA
                     SPEMFDLDDDQRSVVDWQRQTLSQLVATAQNFGAAKNRMVVVMSIKPTKEWDKARRGT
                     ISDQQVYELPLVQLGRAMVEALENLHLMRLKNVHCLSLTELSEVIRVAWDLDAGDAYN
                     ERRLSGELPRTDADIVTVTVDDIMDKRLAYGPDQINQVLTPLYAWPEKIVEVGDDYIR
                     LDDTWISVLRMIKLPREIRVDQFNTLHHLPKPGRWTSMAMVGEAISGNMETTQLVIQQ
                     SAIINWMSAFQRNRVVKDPRMMRRQRNLATQAEQVSSDSLGQRFNFLYSCSASSLEEL
                     YSVRRELKASLMARGFPCRVVKGRSRQLDAAISAALGVNRL"
     gene            complement(12978..>13664)
                     /locus_tag="VFG56_02935"
     CDS             complement(12978..>13664)
                     /locus_tag="VFG56_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622864.1"
                     /translation="MISAIFGGFTMLIVVVEVFFLNLWLLFLLLGYPLVWALRPIGRV
                     TNIIFHTFNCGLFTVLVAPPIMVFWLVLSIGAVRVIPFADNPLGAAVFTQLGGLAAAI
                     TPIALAVMSYRRSSHVFGRVDSEMDGAFDINRLPPVTTKQMRDDVDETYSATVIPLHA
                     RVSDNLVSNGDTAVQDAVDTVKRHAASAAASKAATAIHPIAGVAVAAGYSAYEKHQAN
                     KSEEEGAVNE"
BASE COUNT         3513 a         3629 c         3407 g         3115 t
ORIGIN      
        1 tctctggcaa cttattcagt gtagctgccc ctagttttgg ccttcgacct gagccgctgt
       61 tcggtctacc gctgctgatt ttgattgtgc tcggtatctt acagatattt cgtgacaatt
      121 tctcggctcg ggcttatgtc ctcatcggct ggctagctgt cttcttcgta atcttaagta
      181 tcaatgccga ccatgtactt gaccttttcg taccggccgt acttcttttg gctgtaggtg
      241 tagaaaccct gttgcacgag tggtacaaac tattcccacg aaatccatat gcgagaatcg
      301 gcgccgttat accgctgact atcttgattc taggtatatc tatcagcgaa gttgcccgct
      361 attttgacgg ctaccgatac ggcgtcgatt attccaatac caactactca ttggcgctca
      421 aacccctcag gaaccagcta aaccagtcga gcaatcaatc gacttatttg atcgctccga
      481 ccgaccaatt ggatttttac aaactactgg caaatcgctt tgacaacctg accgtcagtg
      541 atgattatgt accggctagt agtagtcaat tagtgatcgt tttgcatggg actcagctag
      601 aggcggctta tccggtagat caaatcaaac acattgagac gacaccactc agacaccagc
      661 ctctattgtt tagcgtctat caaaccaatc agtaaaactg ttacaatata gctagtaaaa
      721 cgaaggagta ttcatgctgg atcaagccaa agctgtgatg caaatgaaaa agatgcaaaa
      781 agaactggcg cgcgaaattg tcgaagttga agctggtgac ggtgccgtgg ttgtccaagt
      841 taccggtgag atgaagctaa agaaggtcaa catcgacccg gagtcggttg atttggacga
      901 tatcgagcaa ctcgaaaagt gggttgagat tgcgatgcga gacgcttacg ccaaagccca
      961 agagctagcc gccgagaaaa tgaaaccgct aatgggtagt ctcggtaact tcggcctgta
     1021 gctaaccgat caaggtaaca atacagtggc cacctaccaa ctgcctaagg ctttgactga
     1081 tctgatcgac gcactcggtg tattgcctgg cgttggcccg cgtacggctg aacgattcgc
     1141 ttcctatcta ctaaaggccg acgacaacct cagccaaaag ctgatcggag ccctaggtag
     1201 cctaaaaagt ggtgtcaaaa cctgcccgat gacgtttgct ctaatggacg ctagcgaaac
     1261 catctcgcca ctctacgaag acaaagcccg caataagcgg ttggtggccg tagttgaaga
     1321 tcccttggat atcgtcgcac tggagcgtac cgatcagttt gacggcacct atcacgtact
     1381 cggcggtgtg atcagcccta tcgataatgt cggtcccgag aagctgcata ttcctgagct
     1441 agtcaagcgc ctggagcaag acaaggtcga agaggtcatc ctggcgacta atgccagtgt
     1501 cgaaggtgaa tcaaccgcac tctatatcca acgacaaatc gacgaagccg gattgaagcc
     1561 ggtaatcagt cgactggccc gcggtatacc ggccgggatt gacctggagt acgccgacca
     1621 aatcaccctc agtcatgccc taaaaggtcg gcaggtgtta tgagtaaatt agtggatttt
     1681 atcgaccgcc acgacagctt tgtcgtcatc caggccgaaa atccggacgg cgactcactc
     1741 ggtagcgccc tcggattaga ggctatcatc gcggaaactg gcaagtcagt ccagctatac
     1801 tgcccagtcg aaattccgaa gtaccttcgc tacgtaaacg gctgggaccg agtagaggca
     1861 gactttccaa cccaagtagc cgccgctatc atcgtcgaca ccagctccag taccttgctg
     1921 tcaaaaactc tagacgatcc agtcgccaaa catttcctta ccgaccagcc tgtattggtg
     1981 gttgaccacc acgcggtggt aggcggggaa ttaacctttg atcacgacca gctgattgat
     2041 actgaagtcg ttgcgaccgg tgagctaatc gctaaagagg tgcttaagtc caaactgaaa
     2101 ttgccggaag atgccgccac ggctttaact atcgcgatct tatcggacag cttaggtttg
     2161 actaccgaag ctactacagc agctagctac cgcaccatcg ccgatctagt ccagcacggc
     2221 gccaaaccgg ctgaaattga atcagcccga cgcgaattta tgaaaaagtc agccgatatc
     2281 ctggcttaca agggtcgttt gattggaagg atagaatacc atttggacgg acggttggca
     2341 cttgtccata taccttggga ggaaatcgag gcttattccg atcaatacaa ccctagtgtc
     2401 ttggtactcg atgaaatgcg tctggttgag ggtgtagatg tggccgtagc catcaagact
     2461 tatcccgatg gtaaactaac cggtaaatta cgctccaacc ggccggtatc cagtcaggtg
     2521 gccgggttct ttggcggtgg tggccacccc tatgccgccg gctttcgtat ttatgagagt
     2581 tacgacaaca tgttgccaga actagtcgaa gctacgagaa aggcgctatc atgagcctgc
     2641 agctatacga taccgcctct agaattaagc gggagttcaa gccgcaggta gacaacctgg
     2701 tcaagctata cacttgtggt ccgactgtct acaacgagtt gcagattggt aattggctgg
     2761 cttatctgcg ctgggatcta ttggtaaggg tgatgaccct aagcggctac cgtatcgatc
     2821 gagtcatgaa tatcaccgac gtcggccact tagtgtcaga cgccgatgac ggcgaggata
     2881 agatggagaa gggagctcga cgtgaaggtg tcagtgcctg ggagatatcc aagcgctata
     2941 ccgacagctt tgtaaacggt ctggcggagc tcaatctgat caagcccgac cacttagcta
     3001 aagcaaccga tttcatcgcc gagcagatcg atttcatccg aggcctggag gccaaggggc
     3061 acacttactc gatcgacgat ggtgtatatt ttgatactgc caccttcccg gattatgcca
     3121 aatttgctcg acttgatctt gatgaccaga aagccggcgc cagggtagtc tacaatccag
     3181 acaaacgcaa tccgtcagat tttgccctat ggaagttcag ccctggcgat aaacaacgcg
     3241 acatggagtg ggatagccct tggggtaaag gcttcccggg ttggcatatc gagtgctcag
     3301 ccattgctcg acattacctg ggtgacaccc ttgatataca caccggtgga atcgaccaca
     3361 taccggttca ccacaccaat gaaatcgccc aaagcgtcgc tttgaccggc caaccgctgg
     3421 caaactactg gctacacgct gatttcttga ccagtgatgg caccaagata tcaaaaagct
     3481 taggtaacgg ctggacgcta caggacttaa ccgataaggg cttccgacta gttgatctga
     3541 ggctattcgc cctgcagagc cactaccgta gcgaaaccaa cttcacttgg gataatttac
     3601 aagccgccgc taaccggctc aataactggc tgtctttagc cgagctacgt tggcagattc
     3661 atgataccgt ggtggacgac gaggataagg ccgtcagcga aagtagccta aacttacgac
     3721 gagccaaaca ccagacgctt caagcattgc aggacgacct aaattcgccg caagcactca
     3781 gcctgataga tcaacagatc gaccaaatca aggccgagat tggcaatctc cagcaagacg
     3841 tactagatga tttcatagac tttatcgaaa actttagcgg cctacgccta aagcaagcta
     3901 caccggatat cgatgacgcc actaagcaac tgatcctgga gcgcctaagg gctcgtgagg
     3961 caagagattt tcaaaaggcc gatgaactac gagaccaatt agttaagact ggcatacgaa
     4021 tgcttgatac tacaagcagt caactgtgga cacgctacta gctagagttt gatctttttg
     4081 agccagccgg agttttcttt cggctcactt cgaccatggt cgtgttcaag ataatcaata
     4141 actaacactc gaatcatacc ggcgatagga atcgaaatga taccacccag caagccaaat
     4201 agctgcaacc caatcaacac cgccaacaac actattagag cggataactc cactgtcttg
     4261 gactggacgc taggagaaat gatgttgttc tctatctgct gatagatgat gaagtagatg
     4321 atgaagacga tggcggctaa gacagagttg agtaacaaga ctaaggcgac gagaatagcc
     4381 ccgacggtag cgccgaacat aggaatcaag ccggagataa aaacaatcgt tgctagcggt
     4441 agggcgatat tagcatccat gtcaaaaacc gcgctcaaga taagaatagt cgccaagcta
     4501 cagaccgccg ctatggcagc aacgatcaac tggccgttga cgtaaccgga aactacctta
     4561 tacatcttgt agacgacatt cttgtgtcgt ttgagcaatg ccttgttctt atatagtgcc
     4621 cagaaccgct ccatccatgt cgggccttct atcagcatca aaaaagcaat aaccaggata
     4681 aacaataaag caactaaacc ggccaggatt gacttcacgc tggcaacgag caaaccacca
     4741 aggttagccg ccaatgcact tacttggccc ttgagttgat cgacgctgtt attaaaggtt
     4801 tgctgcaggc catactcgtc taggaattga tggaagccgc gactgttctg ggttacttga
     4861 tcgatgagtc cgggcacggt agcggcaaac tttgatgatt gctgaatgac cggtggtacg
     4921 accaagaaaa tgatggccgc taatatagct acgactatca ggtaagcgat agcagtagca
     4981 ccgacacggc ttttgccggg caggcgacga gctagacggg taaccggcgg gttaagcgcg
     5041 atggccagga tcaaggctag gacgattatg gtgatcggcg tggtgaggtg gaagaataaa
     5101 taaataccga gaacaaaggc cagggttaca aaaccgagcc ggacaaaggt ttgggtatcg
     5161 atatctatct tgactttcat tggtcttaat tatgtcctac atctagcgct taaacaagtc
     5221 tacagcgtag tgacggcgat aatagccaat agcaccacaa cggacacgcc caaacaagtt
     5281 acggccaacg cgaccttggt cggctcgaaa ttattgatgg tagccggagt ttttttgtgt
     5341 ttttgggatt gggttttagc tttcttagtc atattggttg gattatacaa taacgcttgt
     5401 gattaaacca gttttattac atactgaatg aataatggat ctgcaaaaag aactaaaaca
     5461 aatttttcgt ggtgaagtca gtactgacga cgcactactc accaaatact ctcatgacgc
     5521 cagtttgttt gagctacgac cgcaggtagt ggccgcaccg ctcgacagtg ccgacatcaa
     5581 ggctgtcgtc aaatatgtac tggagcacaa aaaaagccag ccgaatctat cgatcacggc
     5641 tcgatcggcc ggcaccgata tgtcgggtgc agccatcaac gactcgatag tactgtcgat
     5701 gaccgaccac ttcaatcgta tcgaagaact taaggccaac tcagcccgtg tccaacccgg
     5761 tgtctatttc cgtgacttcg acaagaagtg tgcggatctg aaagtcatgc ttccaagcta
     5821 tccggcgtcg cgagacctat gtacggttgg tggcatggcc aacaacaatt ccggtggtga
     5881 gcgtagctta gaatttggca aaacagctaa ttttgtcgag gaactccgcg tcgtcttagc
     5941 cgacggtaac gaatacacca tgcgaccgct caataaacat gagttgatga aaaagatcgc
     6001 ccaaaaagac ttcgagggtg ccctgtacgg acaagtattt gatctactgg agaagaacta
     6061 tgatctgatc aaaaacgcta agccggccgt atcaaaggac tcaaccggct atcacttatg
     6121 ggatgcttgg gatcgtgaca ctggtatttt tgacatgact aaaatcatcg tcggcgctca
     6181 aggaacccta ggcctggtga ccgacattag gtttcggtta gtacctagga aagagttctc
     6241 cggtttgctg gttggctatt tgaaaaatca agatcagctc ggcgaaatca tcaacaccgt
     6301 cctcaagcat aaaccggcga cctttgagag ctttgatgac aatacgctat ggctgagctt
     6361 caaattcttc ccagctttta tgaaaaaatt aggattctgg ccttgggtta aacttgcaat
     6421 acagcttatc ccggacggcc tagccctgat aaaaggtgtg ccgaaattga tcctaatggt
     6481 tgagtttaat ggtcactctc aagaagaagt tgatgccgcc atcgataaca tgaaagagga
     6541 cctagataac tacaatttca cctatctaga gaaggatgac actctagcaa agagcaataa
     6601 attctggatc atgcgacggg aaagcttcaa cctcttgagg agcaaagtca aggacaagca
     6661 cacggctccg ttcatcgatg acctcatcgt gccaccacca gagctgccgg ttttcttgcc
     6721 gaaattacgt caaatcatca aaaagtataa gctcttagcc accgtcgcag gtcatatggg
     6781 ggatggtaac tttcatatca tcccactcat gaaaatcgaa gatccgcacg agcgagccaa
     6841 actccagccg gccatgaaag aagtcaatgc cctggtacta aaacatcacg ggtcaatctc
     6901 cggcgagcac aacgacggtt tagtgcgtgg cccatggctg gagactatgt acggagctga
     6961 gtacttccag gtgctaaaac gaactaagcg catctttgac ccacaaaata tcttcaaccc
     7021 gcataagaaa accgacgcca attgggacta cagcttctcg cacatccgac aaaacttcga
     7081 tcaatagttt ttgggcatga aaaaggccgg gatacgttcc cggccaagac gcccgagttt
     7141 tgatagaaaa ctcgagaaac tattttggct caaaacctta cggccttaga cctttcgagc
     7201 ggtagaaggc ggctgggtca actgaaccct taccgccaac tgcccgacct tgcatagccc
     7261 aatccagatc ggtggctatt gtcacgtgga catgacaacc ggtagaattg ccagtcgatc
     7321 cctcgtagcc gatcaattga ccggtcgaga ccgtctgacc cacgccgata cccggggcaa
     7381 aacccgtcat gtgaccaaac aggctccaca gtcgcggcgc atgctggatg acgataaaat
     7441 taccccaacc actatcgtaa ccggctctga caatgcggcc gccagagtcg gcttgaaccg
     7501 gtgatccaca cgctccagcg aaatccaaac cgttatgccc gttcgggtag ggacggtatg
     7561 ggtcggtgag tgtgtaacta ccgggctcaa gcggtaacac ccatttgcca tcaccgccag
     7621 agacaaacga cacgtcgata tacaatacgt cgtgatccga cgccgagccg gtctccagcc
     7681 atctccagtt gaccacgtcg acccccttgg agacgtagac atggtcgatt atccgacctt
     7741 tgacctgacg gtcggcgttg gcctgtgcat cggtagtctt gcagccagtc tttccctcca
     7801 tcgcatcgta agcgttcagc atgtcaccgg atgccgtcat gatgcagtag ggcaagcggc
     7861 tcttatcgtg accgataagg tagctgtccg atgtcggccc gccgaaacga atgtcgaaag
     7921 ttgaattgaa gtcaccggtc aagaaaaccg gcaacccttg cttcaaccag accttggcgt
     7981 cggctaagtg ctgatgagcg ttagcttccc gccagcgagc actttggccg tgccggccat
     8041 cggccggatc gtgggtgttt tggacgatga acaccgtctg cgagtaattg tccttcaact
     8101 taacccaggg aatatgtaga gggctgccgt tgaagtaacg atagccggtt gatatgccgg
     8161 catctagttt ggtcaaccgt gcctggttcc agatgatcga gttttcgaca ctgtggtgaa
     8221 cattgtgcgg gctagacgga tagataccgt actgtccact caattcatcc accaaagcct
     8281 ggcgctggtt agactgcagt tcctgcaagc caaccacgtc gagattgttc agctgaacca
     8341 ttcccgccag ttgccggacg cgttcagggc catgagcctc atggctggaa ccacgaacat
     8401 tgagtgtggc tatgcgcagg ttcggacctt ggcaggtcca gtccgccgct acagtcgaca
     8461 ctccggtcga ctgctgactg ttggtaacag ccgccgccac agtggcttca gcctcgtgta
     8521 cccaaagcgc ataacgctca ggaaaggccg atccctcaat ggcctgagca gcatccccga
     8581 tcgacatctg ttgccaaccg acggtcttct caagctcttt gtacatccgg ttgatggcgt
     8641 agttggtatc caagatctgt tcaggtgtac cccagtagta tggccctacg ctgggtcgca
     8701 tctgaaacag tccaaggcta tcactatcac cataaggcag attgagcagg gtggattcca
     8761 cagtcgacgc aacaagcaaa accttccaag ctcggcttgg caggcgcctg ttaacaccga
     8821 taccgatgat ctcggcggca ttcttagcct gagctggctg catcgggcca cccttgaccg
     8881 tcaagttagc cgagttgagg ctaacattgg cggcctggac cgtgatgcct acaccactag
     8941 agtcaatctg ctgacacatc tgttgtcgag cactaaccac gccccctaaa ccaaataggc
     9001 tggctagtat cacagtaaac gccataatgc cggcgagact aagcgctata gcaattttta
     9061 tggtgcggct cacgacccac acctcctctc tactgagtca gggaatcaag tagccaaccc
     9121 ttggttggtc gcttgatcca cgtcattcta actaactgat cataccggtc aatcgtactg
     9181 ccggtctcat cggttttagt catggtcacc accaccggca attcaaccgt tggtggctga
     9241 ccataaactt cactaggcca aatcgattgc accgaaccgt gtacttgaaa cttaccacta
     9301 cgaatcgctt gatcagcctg gctatcggtt aggccgaaaa aggtctttcg cataaatcgt
     9361 ggcgttgcca agctacgcac cgcccgtctt tgactgagcg ccgtcaacga acgattgtag
     9421 tcatagaagg cctggacaaa agccctggtt cgagcttcaa gctggctcaa cgactcatgc
     9481 ttgtcttcaa ccgtcgccac cggatcggcc gatgtcgaag ccgaggctac cgcctgctga
     9541 ggcggtttat cggcatcggt ccgacccggc ctggcaaggc cataaacaat ggctaacacc
     9601 agcccgacga ttactaggcc gaataaaacg taacttggct gaggcagcca agtacgctta
     9661 ctgaaccgtt tcatgatcca gtccaagata gcgaatgccg ttttccttgg caaaacgctt
     9721 catgtcatcg acatccagga tgctcggctt gttttgcatg cggttagccg ctgcatcagt
     9781 cggcagcaag ggcagctcac tgggagttgc aaagacccgt ccaaaaatca tcggggtctc
     9841 ttgacctagc ttcacaccaa agtgatactt cggcatccga gtcaactgcc gggtgtcggc
     9901 atctgacaac tcgtaacgat cctggagttc acctaggact tgcttcttgt tcggctgtga
     9961 gccgatcatg acgaaaccta agttgtcgat aatcgtctcg ccgaggttgt atagctcgct
    10021 acccaccgca ccacgtcgga tactgtgcaa gtagtgagaa gctgaaaggt tacaggtatg
    10081 cacaccacga ccaatctcac tgaggtgaga gtgctgagtg gcgtagacca gattacccat
    10141 cgatttgtgc ttctcatcat ccaactccag gtggggaagc agatcgagcc ggttgccttc
    10201 gatggccgcc agcttgattc gagcgtcgat ggtccgcatc aacgtctcgc cgtcgggcgg
    10261 gacgttgcgc caatccttgg ttacagcccg ctgggtcatc acgtcgtaca gcgagtgctc
    10321 gtcgccgaac atgtagccgt acggaccgtt gatgacaccg tccagaaggg aggccacata
    10381 gaccgcactc ttcttgatgt cctccagaca ggcgttgttg tcctgcctgt ataggtcgtc
    10441 aagttgagcc cgagtagtct tttgcagttc cacgctcaga cgatcatgct tgccctccag
    10501 ctggagttta atcttgtcgt cgaggctgcg gtagtagccg ttgatgtctt cgtcagacag
    10561 agtcaagagc ttgtacagca agctgtgagg tgaccacaag ttcttgtcca tcgacatcat
    10621 ggcgtagacg gccgcccgta aggccacgcc gtcattgcca atcagacgat tagcgccgtt
    10681 tggcaagaag gtctcacata gaatatgcgc gatctcgagt agcgccaagg cgttagtcga
    10741 gccgtcgtgt tggatgaaca gccgattatc aaacacgttg acacgcattt cattgatacg
    10801 gaacaccttg ctacgatagg ctcgactgag cttctcgtat tcaccagcgt caccttcagg
    10861 cttgtagtca ttgatggcaa tgcgcatcat ctgataaccg gctgatgtca gcattagccg
    10921 ggatgccatt atcttcatgg ttgaagattt gccatcacct ttgtggccaa ggatcaagcc
    10981 gaaagtcgag ttgatgacac cgttgagctt aagcagccac ggatcgaaca acagcagttc
    11041 tttcgacttc atgtccacca tcaaaggtat accccgtttg agcggatggg gttgcgcaaa
    11101 gaacggatgg cccatagcca tccactccgt cggccccgcg aaaacactcg atcgaatagc
    11161 catggatctc tccctcctcg ggtagaaaag gtttgtcttt ttaaggttcg aagcgtggtt
    11221 tttacacgac acttcggcta agtctaacta agggtaaaat tagcccaaat gtagtgtttg
    11281 ccccagggcg ccggaaaaac cggcaccctg gggctggcac ttttgtgcag atttgctaca
    11341 aacggttgac tcccagcgcg gcactaatcg cggcatcaag ctgacgcgat cgacctttga
    11401 ctacccgaca agggaagcct ctggccatca ggctagcttt tagctcgcgg cggaccgagt
    11461 aaagctcctc caagctcgaa gccgaacagg agtagagaaa gttgaaccgc tgaccaaggc
    11521 tgtccgacga aacttgctcc gcctgagtgg caaggttgcg ttgtcgccgc atcattcgcg
    11581 gatctttgac cacgcggttg cgctgaaagg cgctcatcca gttaatgata gctgactgct
    11641 ggatcaccag ctgagtcgtc tccatgttcc cggatattgc ctcgccgacc atcgccatgc
    11701 tcgtccaccg cccaggcttg ggtaggtggt gtagagtgtt aaactgatca accctgatct
    11761 cgcgcggtag tttgatcatc cgcaacactg agatccaggt gtcgtctaac cggatatagt
    11821 catcaccgac ttcgacgatc ttttccggcc aagcatacag cggagtgagt acctgattga
    11881 tctgatccgg cccgtaagcc agtctcttat ccatgatatc gtccaccgtc acggtgacga
    11941 tatcggcatc agtacgcggc agctcaccac tcagcctacg ctcattgtag gcgtctccag
    12001 catccaaatc ccatgctacc ctaatcactt cactgagctc agtcaggctg aggcagtgga
    12061 cgttcttaag gcgcatcaga tgaaggttct ccagggcttc aaccatggcc cgacccagct
    12121 gaacgagcgg taactcgtaa acctgctggt ccgatatagt tcctcgcctg gccttgtccc
    12181 actccttagt cggtttgatc gacatgacaa caaccatacg attcttagct gcaccgaaat
    12241 tctgagccgt ggctactagc tggctcaaag tctgacgctg ccaatcaact actgatctct
    12301 ggtcgtcgtc tagatcaaac atctcagggc ttgccacgac tgggttgacg ctttcagcta
    12361 ggtagtcgtt cagatcgtta aaatcatcgg gacgaccata cctgagatac gagacaccaa
    12421 tcttgatccg gctttgggcc gccacctggt tagtggcggc cgccagctcg ttgacgatgt
    12481 tgtgctgctg catgacgtcg agactcgaca tccgaccgcc gtccgccacg ataaagatgt
    12541 ggtcgagatt tcggtcggtc tgatgcaaga caccgaagct ctcgccaagg gcctgcacca
    12601 tttccaattt aaccgggata acttgacgct tctcccggtt ttttcggtat cgcttctcac
    12661 gtagtattcg tcgcaaccag ccactgtcgt cttcatcgat ccacagcaca tctctcagcg
    12721 ccaaatggat gtagaagaag ccgtataggc cggttgccag ctcccagtaa agccgtccgt
    12781 actcagttct gatgtttagg ggcaccagga cgaacaggat agccatcatc gccagaaaat
    12841 cgttgatgta aacgaacagc gctatagccg cgagtagcga cgttacgccg aaagcaatat
    12901 caacgccagt ccagcccgcc caggatcgcc tctgaagctt attctgaggg atactgaact
    12961 tgggcaacgc ttcgaggtta ctcattgaca gcaccttctt cctcagactt gttggcttgg
    13021 tgtttctcgt aggcactata accagctgct acagctacgc cggcaatcgg atgaatagcc
    13081 gtagccgcct tggaggccgc cgctgaagcg gcgtgtcgtt tgacggtatc gaccgcatcc
    13141 tggacagccg tatcaccatt actcaccagg ttatcgctaa ccctagcgtg cagcggtatt
    13201 accgtagcgc tgtaagtctc atcgacatcg tcacgcatct gtttggtggt taccggaggc
    13261 aggcgattga tatcgaaagc accatccatc tcgctgtcga cccggccaaa tacatgacta
    13321 gaccgacgat aagacatgac agccagcgca atcggcgtga tagcagccgc taagccaccc
    13381 agttgggtaa aaaccgccgc accgagcgga ttatcggcaa acgggattac tctaaccgca
    13441 ccaatcgaca acaccagcca gaacaccata atcggtggcg ccaccaagac ggtgaacaaa
    13501 ccacaattga aggtgtggaa gatgatgttt gtcacgcgtc cgatcggacg cagtgcccag
    13561 accaacggat aacccaacag caggaacaga agccatagat taaggaagaa cacctcaact
    13621 acgacgatca gcatagtgaa tccgccgaat atcgcagaga tcat
//
LOCUS       DASSBW010000099        14463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U905 k141_1202408, whole genome shotgun sequence.
ACCESSION   DASSBW010000099 DASSBW010000000
VERSION     DASSBW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963078
            Sequence Read Archive: SRR25158421
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 14463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:04:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 603
            CDSs (total)                      :: 581
            Genes (coding)                    :: 578
            CDSs (with protein)               :: 578
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14463
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202408"
                     /isolate="SMAG_U905"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.575 N 129.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFG56_02940"
     CDS             <1..485
                     /locus_tag="VFG56_02940"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HET6622865.1"
                     /translation="QLESAVASFTAAAAQRLRNQESICSGIITSLTSRVERRQNRISR
                     LTSLSEPTADTGRLISSALAGLAVAYDEDKAYKKASITLVGITERTAWQLSLTDPDQQ
                     RQRRQRLMEEVDRLNRRFGGGTVSYAVEDKLSANWRSKHEHRSPRYTTRWTDLPLLKA
                     "
     gene            648..998
                     /locus_tag="VFG56_02945"
     CDS             648..998
                     /locus_tag="VFG56_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622866.1"
                     /translation="MAQTCFQKIGHILKDMVDECGGAYISTREDYIEFIRREAPTASE
                     LAVQHFTCSLRRHGLTSRGYHERSNDVVVIFDTTKFDFEQAVAHVEMMGLPEAVQIRL
                     FELDVARPAEAVTA"
     gene            998..1177
                     /locus_tag="VFG56_02950"
     CDS             998..1177
                     /locus_tag="VFG56_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622867.1"
                     /translation="MADKGRDGRFHGKPTGSRKALPSTSGGQTPVGFSHQSPPPKTGN
                     AGRQPKIRDPFGRRK"
     gene            1513..1587
                     /locus_tag="VFG56_02955"
     tRNA            1513..1587
                     /locus_tag="VFG56_02955"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Glu,seq:ttc)
     gene            1786..3195
                     /gene="pyk"
                     /locus_tag="VFG56_02960"
     CDS             1786..3195
                     /gene="pyk"
                     /locus_tag="VFG56_02960"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET6622868.1"
                     /translation="MPMIYKRTKILATLGPATNNYDSIEELILSGVNGFRLNFSHGTH
                     EERDQQITWIRQASDKVGKPVAILQDLQGPKIRLGLLNDEIQVKTGDELILEYGADHH
                     DNVLPVQYDLSEKVKPGQPLYLFDGKVRSQVTKVTGKAVTIKIQNDGTLMSRKGLNLP
                     DTNFGGDIITKKDLADIEYGAKADIDYVALSFVQTADDIEDLKAKLKKLGSTAKVIAK
                     IETKSAIEADNLEAIIRVSHGVMVARGDLAVEAGAEVVPIVQRDIIGLCQKYGKLSVV
                     ATQMMASMVDNPDPTRAEVSDVAHAVITGADCVMLSDETANGKYPLETVQAMKKVILY
                     SQEKAPLRPIYFKEGCALDKSDAISSAAVTLADQLPAEAIVAETKTGDTAFSIASHRP
                     SLPIISVTSSPRAAQQLALLYANKSFLRPDGEKAGLTQAQELRQQDFFSQPTTVVLVS
                     GRQPGVSGATDTIRVRYLE"
     gene            3208..4425
                     /locus_tag="VFG56_02965"
     CDS             3208..4425
                     /locus_tag="VFG56_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6622869.1"
                     /translation="MSRVGLNKKTIVQVPLDGQTVLVRADYNVPLDSKGQIADDYRMQ
                     MSLPTLKNLLSRGCRVVICAHLGRPDGKPNSTYSLEPVAHHLVELLGQPVKFVSDCVG
                     DKAMQASRRLKKGEVLLLENLRFHPEEEANDRQFAKSLAKASRASYFVQDGFGVVHRA
                     HASTSAITEFLPSVAGKLLEKEVTTITRAMEKPERPLVAVLGGAKISDKIGLVERFVK
                     IADRVVIGGAMANTFLTDKGVDIGASLSETDLQDVIRLIYKAAGDQTDKLILPEDVAV
                     GKTTDQAEPRREVELDQIAADDKILDIGPKTIEKVAAAVDTAKTVVWNGTLGYAELDQ
                     FAHGSARTALELARRRGQVESIIGGGDTADFVINWDANHGASFSHVSTGGGASLELMA
                     GQKLPGVEALLDD"
     gene            4473..5261
                     /gene="tpiA"
                     /locus_tag="VFG56_02970"
     CDS             4473..5261
                     /gene="tpiA"
                     /locus_tag="VFG56_02970"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET6622870.1"
                     /translation="MQQRKSLIVANWKMHLGMHQASLFAHKLADKVKVRQDVEVVLAP
                     TSLVLQSLSLQVNRRQFKLAAQNCYWRDEGAFTGEISADMLRGIADYVIIGHSERRHV
                     FAERDRDITHKVQAVIRNKMKPILCIGETAHERADGETSHVLHDQLVGGLLNVVSDEI
                     EDVIIAYEPVWAIGTGDNAAAQEVQEAVKLIRRQIKALYGAKAADKVRVLYGGSSNAH
                     NAADYLKLDGVDGLLVGGASLKADEFATIVDKAHQAQAVKRDEG"
     gene            5261..6493
                     /locus_tag="VFG56_02975"
     CDS             5261..6493
                     /locus_tag="VFG56_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622871.1"
                     /translation="MKNDIDFDELDKAVGAVLNTGAAAKPSDDAKPQPVVPTTPAKPV
                     SNSVEEAIPVAKATVSPEKPSTSKKPAKPASPALKRSSGRFMDMVHPSADMKKSNTPS
                     VKRDGNKLQPISKDIKAEDSAPEPTSPAPAVMSKSDYEPGPPAADLAATGFVSMPDDD
                     KAANAKSEWPDPLEVDKPTDGSPVEEPTMLASSESAVPEASQSADGPFLSDQKVEKRP
                     LGAFALDGKSDEATAEPIQTETNPVVQPGETSSSPVPQELQPEAVKVEASDVPEPAED
                     DAPLSDKLGDTSLKSEASTTTTEQTKEHGKLDDASSKTDDNQAEPSSDTKSPVDTVNL
                     SGQSIAPQYKPQATDNDSAEAASSVFDTKEYHQPLAPSAAKHGLKKVTSVIGLIILLV
                     LIGAAIGYAYYVYRTGGF"
     gene            6537..8654
                     /locus_tag="VFG56_02980"
     CDS             6537..8654
                     /locus_tag="VFG56_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6622872.1"
                     /translation="MFDGLNEQQLEAAKHTDGPLLILAGAGSGKTKTLTHRIANLIHS
                     QSVDPVNILAVTFTNKAAKEMRERLGILLGVDGDQRSFMPWMGTFHSICVRLLRIDGP
                     QIGIPSNFVILDESDRLSLIKQAMKSLSISEKNYTPRSMASLISSAKNEGLSPDEYAA
                     TARMPLNKTVAAIYPAYESLRRDAKALDFDDLLVETVRLLKTVADIRRKWQEQFNYVL
                     VDEYQDTNTVQYRLIKLLLNGKHNLCVVGDDWQSIYSWRGADFTNILNFERDFKNVKV
                     IKLEKNYRSTKPILEAAHNVITKNQQRTAKKLVSQLGDGQPVQILHVQSEIHEAEAVV
                     TRIKTAIDLRLRQYNDFAVLYRTNAQSRALEEVFMRYGLPYKIIGGTRFYDRAEVKDV
                     MAYLRLVYQPADRASFLRIVNLPARGIGKVSLGRFLDWHDSIQLDIVGCLNQVELCPG
                     LPTKALNGLKALGRILTDLLERHDDSSLFELINAVIKRTGYLDYLDDGTPRGDDRVAN
                     VKELLSDAEQRPDTGLAEYLEEVALVSSQDSEASEGAVTLMTLHAAKGLEFPVVFMVG
                     MEEGLFPSGQSVYEADKLEEERRLCYVGMTRAKEELFLTTASSRLTFGARSYNPPSRF
                     LADMEVAETSAAQVSLPTPSLASNEPRLVLDEVDLQIGDKVKHSLFGVGTVTDLDGSN
                     VAVAFAGKGVKKLNTAFAPLEKL"
     gene            8731..9993
                     /locus_tag="VFG56_02985"
     CDS             8731..9993
                     /locus_tag="VFG56_02985"
                     /inference="COORDINATES: protein motif:HMM:NF037025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HET6622873.1"
                     /translation="MTISELRYLFKKGWIIIAATLAIALTWTVQGALADTASTPSLGE
                     HLVTIYDSGQEKSLVTKAPTVGEALEEAGVELSDKDQVEPAVDTPLLSKNYHINIYRS
                     RLILIVDGSKRLSVVTAAQSPESIMKAAGLDLYDEDRAELKPIDNILSGGAGLELTID
                     RATVFKFNLYGKTFVDRTQAETVGQLLADKGVTLGPKDGVTPSSSTPIETNMLVHVWR
                     NGKQTVTQEQTIKAPVKKIYDYDRPLGYKQVKVAGQDGIKQVTYEIVMKGGRQVSKKV
                     IATVVTKKPVTEQVVVGVKSGNPSENAAIIWAYLTSHGFSPEQTAGIMGNLQQEHGFK
                     TSGDGLAQWTGGRKANLMSRDDPYSLYTQLDFMLDELDGSYAEGAIKSTNSIEQATVI
                     FQDHYERCGLCAESKRISYAYSIYNRFN"
     gene            9995..10756
                     /gene="rsmA"
                     /locus_tag="VFG56_02990"
     CDS             9995..10756
                     /gene="rsmA"
                     /locus_tag="VFG56_02990"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET6622874.1"
                     /translation="MPDKSLGQHWLKDREVLRSIVDLAEIKPTDTVLEIGPGLGTLTS
                     TLLAKAGSVIAVEFDKHLATKLPDQFPGKNLTVINQDVLQFDLGSLPPGYIVVANLPY
                     YITSKIIQYLSEADNSPVSAVLLVQKEVAERLAAQPGDLSLLAVATQIYFEVSLGPVV
                     GAELFTPPPKVDSQVVVLLRRQRPLVEQAERGRLFQLVKAGFSNRRKKLRSSLAGGLR
                     MPKTEVERLLGDAGIDANLRAQDLAIDDWLRLARL"
     gene            10760..11467
                     /locus_tag="VFG56_02995"
     CDS             10760..11467
                     /locus_tag="VFG56_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HET6622875.1"
                     /translation="MIHSHQPTSLPADLIVGLSSIEDGDMKHSPYSDPAAVLMNRQEF
                     LHRIGIDIRQTNLVRIDYKSDDFCRYRIAEDSDNGVSMQASHDTAPADALATRQAGQA
                     LFLPIADCCSLVLADSESPAFMLSHLGRHSVSQLGGRKSVEFLVDNLGATLKDIKAWL
                     GPTVGAATYPLFDFGGRSLQNVIVEQLMTAGLKREQIEICPVDVAEDERYFSHSQFKK
                     GKRPYDGRFAVVAMRQS"
     gene            11497..12987
                     /gene="metG"
                     /locus_tag="VFG56_03000"
     CDS             11497..12987
                     /gene="metG"
                     /locus_tag="VFG56_03000"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET6622876.1"
                     /translation="MTKKSLYITTAIPYANAKPHIGNALDYLLADIWARYQRQQGKEV
                     RFQVGTDEHGNKIAQKATEAGKDPKAYVDEMYKNFEQLMQAVGADYTDFVRTTDIHHR
                     AAVQYIWQQLEPYIYKGKYEGWYCTGHEAFFTDKEAAEMEGVCPDHQQPLERISEDNY
                     YFKMSAFSDQIREAIDKHKLEIVPSFRKHEFLNLLDTGLNDVSVSRPKKNLTWGVPVP
                     GDDNQVMYVWVDALSNYLTVLGYPDDDDWQNYWPADIQVIGKDILRFHAAIWPAMLLA
                     LGLPLPKKLLVHGFVNVGGAKMSKTVGNVVDPMEIIDNYGVDGFRYYFSRHIPTLDDG
                     DFTWEKFENAYNNELANELGNLVSRVGKMINNYQAGVIGDAIKSEHDAAIYHDAMKNL
                     EFNKAMDEVWLMVRGLNQYLEEVKPWQVAKQRETDAEAESHLGEILAHAAGTLLQLAA
                     LLDPFLPTTAKAINDIFSTGVVKLPDSVMFEKKYLHTPDPKASNNA"
     gene            12980..13786
                     /locus_tag="VFG56_03005"
     CDS             12980..13786
                     /locus_tag="VFG56_03005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6622877.1"
                     /translation="MPSQASSKESQPAELFTDTHCHIHEANYPEAEAALMRAHSVGIE
                     SLICVGTDEKTSAEAVQFAEHHDNVWASVGLHPHDAKSGISAIKYLEKLIVTSPANKL
                     VAIGECGLDYFYNNSPREKQIDMLHAQLELAEKYGLAVIFHVREAFDDFWPIWREHGI
                     KRAVLHSFTDNQANLDKAVAEGLFIGVNGISTFARDKQAMYAAIPLENLVLETDSPFL
                     TPIPHRGMVNEPAFVTHVAKHLANLQSIDLSELSRATQRNATKLFFKTNS"
     gene            13834..14223
                     /locus_tag="VFG56_03010"
     CDS             13834..14223
                     /locus_tag="VFG56_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622878.1"
                     /translation="MTEQTQLYTLPGGAEQSRRLFDDQSNPELVEARNIAQASEEFRG
                     DLREVRSTNPEDTFRLDEEEEPTSGPLWDQQAREVRLLEQRQHHARTTHQERLTGAIQ
                     EMSPAQNIADIADTTIALYNFRTMKAA"
     gene            14238..>14463
                     /locus_tag="VFG56_03015"
     CDS             14238..>14463
                     /locus_tag="VFG56_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6622879.1"
                     /translation="MSDLLTKTIQLIAGDSFSKRPLKKLTERQLIELESEIGRELFGP
                     IPKGHRREFFCLDAHTWIWYEEWREEGQIKS"
BASE COUNT         3812 a         3541 c         3745 g         3365 t
ORIGIN      
        1 atcaactgga gtcggctgtg gctagtttca cggccgccgc cgcacaaaga cttaggaacc
       61 aagaatcgat ttgttctggc ataatcacta gtttaaccag tcgagttgag cggcgacaga
      121 accgtattag tcggctgact agcctaagtg aaccaacggc tgacactggt cgcttaatca
      181 gttcggccct agccggtctg gcggtggctt acgatgagga caaagcctat aagaaagcta
      241 gtatcaccct ggttggtatc accgaaagga cggcatggca gctatcgctg accgatcccg
      301 atcaacaacg ccaacgtcgt cagcgtctca tggaagaagt tgaccggctc aatcgtcgct
      361 ttggtggcgg caccgtaagt tatgccgtcg aagacaagct gtcagccaac tggcggagca
      421 agcatgaaca tcgtagtccg cgctatacca cccgctggac agacctacct ctgctaaaag
      481 cgtaagtttg ttgataacac aacaaaaata tgtggacaat tcttgacgta ttttgtcaag
      541 gggggtatac caggtataac gtttagcgaa agtcaccaat ggctttcgac tgatctcatc
      601 gagggagatg ggttagttta cgttttgaca atcagggaag gtacacagtg gctcagactt
      661 gttttcagaa aatcgggcac atcctcaagg acatggtcga cgagtgcggc ggcgcctaca
      721 tctccaccag ggaggactac atcgagttca tccgtcgaga ggcgccgacg gcttccgagc
      781 tggcggttca gcacttcacc tgcagccttc gccggcacgg cctgacatct cgcggctacc
      841 acgagcggtc caacgacgtg gtcgtcatct tcgacacgac caagttcgat ttcgagcagg
      901 cggtagcgca tgtcgagatg atgggtttgc ccgaagcggt gcagatccgt ctgttcgagc
      961 ttgatgtcgc gcgtcctgct gaggcggtga ctgcctgatg gctgacaagg ggagggacgg
     1021 ccgtttccac ggcaagccaa ccggtagccg caaggctcta ccgagcacgt caggtggtca
     1081 gactcccgtg gggttctccc accagtcgcc accgcccaag accggcaatg ccggtcgtca
     1141 gccgaagatt cgcgacccct tcggtagacg taagtaggta cagctctcgg gccgggggag
     1201 agatcaacac tcccccggcc tacctacctt ccctgatcgt ctcttcctta aggcaggttt
     1261 ttctgttaca ataactcggc gtaaaaacgc cgttttttat gccaaaattt ggtcttatac
     1321 tatgagtgtg agacacattt attcggatca cacatcattt aaattggtgg ccggatagcc
     1381 tcatactatg agtatgaggc tcaaatttct cggaaatccg taaccgagaa gcctcatact
     1441 cgaagtatga gccacattta ttcggatcat ggaaaaatat gtaaacacat ttttccgcga
     1501 tacctcataa atggcctcat cgtctaacgg ttaggacacc aggttttcat cctggcaata
     1561 ggggttcgat tccccttgag gtcaccaaag aaagcgcccc acttcggtgg ggtgttttct
     1621 ttggtgcttt cactagagtg aaagtcgaac cccggttcgc ggttttgcca agcggcaaga
     1681 ccgcgagccg ggcccgacag cgaaaagcag agcttttcga ctggtgggtc gaatttcaca
     1741 tgctcttgtt tgaccagagc gcaatctgtt ctacactata aaacaatgcc aatgatatac
     1801 aaacggacca aaatccttgc caccctgggt ccggccacca acaactatga ttcgatcgag
     1861 gagctgatct tgtccggcgt gaacggcttc cgcttgaact tttcgcatgg cacgcacgag
     1921 gagcgtgacc agcaaatcac ctggattcgt caggcctcag acaaagtcgg taaaccagtc
     1981 gccatattgc aggacttgca gggcccaaaa attagactag gactactcaa cgacgagata
     2041 caggtcaaaa ccggagatga gctgatctta gagtacggcg ccgatcacca cgataacgtc
     2101 ctgccggtac agtatgattt atcggaaaaa gttaagcctg gacagccgtt atatctgttt
     2161 gacggtaagg tacgcagtca ggttaccaaa gtaaccggca aagcggtaac tatcaaaatt
     2221 caaaatgatg gcaccttgat gagtcgcaaa gggcttaact tgccggacac caattttggc
     2281 ggcgatatca tcaccaaaaa agacttagct gatatcgaat acggggccaa ggctgatatt
     2341 gattacgtgg cgcttagctt cgttcagacc gctgacgaca ttgaagacct caaggccaag
     2401 ttgaaaaagt tggggtcgac cgctaaggtt atcgccaaga tcgaaaccaa atcagctatc
     2461 gaggccgata acctagaagc tatcatcagg gtctctcatg gcgtcatggt agctcgtggt
     2521 gatttggcgg ttgaagccgg tgccgaagtg gtaccgatag tccagcgtga catcattggc
     2581 ttatgccaga agtacggtaa gttatcggtt gtcgctactc agatgatggc aagcatggtc
     2641 gacaatcctg atccgacccg agccgaggtt agcgatgtgg ctcacgctgt tatcactggt
     2701 gctgactgcg ttatgctaag tgacgagacg gctaatggca aatatcctct cgagaccgtt
     2761 caagccatga aaaaagtcat actctacagc caagaaaaag caccgcttag gccgatttac
     2821 ttcaaagaag gctgcgctct tgataagtcc gacgccatca gctcggctgc cgttacgtta
     2881 gctgatcagc taccagccga agctatcgtg gctgagacca agactggcga cacagctttc
     2941 tcgattgcct cccatcgacc aagcctaccg atcattagtg ttaccagtag tccgcgagcc
     3001 gctcaacagc tagccttgct ctacgcaaat aagagcttcc tacggcccga tggtgaaaaa
     3061 gctggtttga ctcaggcaca agaattgagg caacaggact ttttcagtca gccaacaacg
     3121 gtggtattgg tcagtggccg tcagccgggt gtttccggag ctactgacac catacgggtt
     3181 agatacttgg agtgataaaa taacactatg tctagggtgg gattaaacaa aaaaacaatc
     3241 gtgcaagtgc cgctggacgg ccaaactgta ctggtgcgag ctgattacaa cgtaccgttg
     3301 gattctaagg gtcagattgc cgacgactac cgtatgcaga tgagtttgcc gaccctaaag
     3361 aacctgctct caaggggttg tcgcgtggta atatgtgctc acttaggccg gcccgacggt
     3421 aagccaaact ctacctatag cttggagccg gtagcccacc acttagtcga attacttggt
     3481 caaccagtca agtttgttag tgattgtgtc ggcgataaag ccatgcaagc tagtcgacgg
     3541 ctaaagaagg gcgaagtgct attgctcgag aatttgcgtt ttcatcctga agaagaggcc
     3601 aatgatcgac agtttgccaa gtctctcgct aaagcctcac gagccagcta ctttgtccaa
     3661 gacggcttcg gggtagtcca ccgggcacat gcttccactt cagctatcac tgaattccta
     3721 ccgagtgtag ccgggaaatt gctcgaaaaa gaagtaacca cgatcactag ggctatggag
     3781 aagccggagc ggccgctggt agcggtgttg ggcggagcta agattagtga caaaatcggc
     3841 ttagtcgagc gcttcgtcaa gattgccgat cgtgtcgtta tcggcggagc tatggccaac
     3901 accttcttga ccgacaaagg tgtcgatatt ggtgccagcc tatcggaaac cgatctgcag
     3961 gacgtcatcc ggctgattta taaggctgct ggcgatcaga ctgacaagtt gatcttgcca
     4021 gaggatgtcg cggtcggcaa aacaaccgac caggccgagc cgcgtcgaga agttgaactt
     4081 gatcaaattg ccgccgatga caagatactc gacatcggtc ctaagacgat cgagaaggta
     4141 gcggcggctg tcgataccgc caagacggtg gtctggaatg gcacgcttgg ttacgctgag
     4201 ctcgatcaat ttgcgcacgg ttcggctcgc acagcgcttg aattagctag gcgtcgtgga
     4261 caggttgaat ctatcatagg cgggggcgat accgccgact tcgtcatcaa ctgggatgcc
     4321 aatcatggtg caagtttttc acatgtttcc accggcggcg gcgccagctt ggagttgatg
     4381 gccggccaaa aactacctgg cgtggaggcg cttctggacg attgaagtaa agtcgtctac
     4441 actaataaat aatcataagt actaaagtgg gcatgcaaca aagaaaatca ctcatcgtcg
     4501 ctaactggaa aatgcatctt ggtatgcacc aggctagtct gtttgcacat aagctagccg
     4561 ataaggtcaa agtccgccaa gatgtcgaag tagtcttggc gccaaccagt ctggttttac
     4621 agagccttag cctgcaggtt aatcgccgac aattcaaact agctgctcaa aattgctatt
     4681 ggcgagacga aggagccttt accggcgaga tttcagccga tatgctacgc ggtatcgccg
     4741 actatgtcat catcggacat tccgaacgtc gccatgtatt tgctgagcgt gatcgagaca
     4801 ttacgcataa agtccaggcg gttatcagga ataagatgaa gccgatcttg tgtattggcg
     4861 agacagctca tgagcgggcc gacggtgaaa ccagtcatgt tctacacgat caactggtcg
     4921 gtggtttact gaatgtcgtg tctgatgaga tcgaagatgt gataatcgct tacgagccgg
     4981 tctgggctat tggcaccggt gataacgctg cagctcaaga agtccaagaa gccgtcaagc
     5041 tgattcgtcg tcagatcaag gctctttacg gagccaaggc ggccgataag gtgagagtcc
     5101 tgtatggcgg tagttccaat gctcacaatg ccgctgacta cctgaagcta gacggtgtcg
     5161 atggtttgct ggttggcggc gctagcctca aggctgatga gtttgcgaca atcgttgata
     5221 aggctcatca ggctcaagct gtaaagaggg atgaaggtta atgaaaaacg atatagactt
     5281 tgatgaatta gacaaggctg taggtgcggt cctaaataca ggtgctgcag ctaagccatc
     5341 cgacgatgca aagcctcagc cagtagtgcc tacgacccca gccaagccag taagtaactc
     5401 ggtcgaagag gccatccctg tagccaaagc aacagtaagc cccgaaaagc catcgaccag
     5461 caagaagccg gccaaacccg ctagtccagc ccttaagcga tcgagtggtc gctttatgga
     5521 catggtccac ccctcggccg atatgaagaa gtcgaacaca ccgtccgtca agcgtgatgg
     5581 taacaagcta cagccaatct ccaaggatat taaggccgaa gattcagcac cagaaccaac
     5641 cagtcctgct cccgctgtta tgtctaagtc tgactatgag cctggtccac cggcagcaga
     5701 cttagcggct accggcttcg tatcgatgcc agatgatgat aaggcggcta acgccaagag
     5761 tgaatggcca gatccacttg aagtagacaa gccgactgat gggtcaccag ttgaagagcc
     5821 gaccatgctt gcatctagtg aatcagctgt gcctgaggca tcgcagtcag ccgatggacc
     5881 gtttttgagt gatcaaaagg ttgagaagcg tccgcttgga gcgtttgctc ttgacggtaa
     5941 gtcggacgaa gcaactgctg agccgattca gaccgaaacc aacccagtgg tacagccagg
     6001 tgagactagt tcttcgcctg tgccgcaaga attacaacca gaagctgtca aggttgaagc
     6061 cagtgatgtg ccggagccag ccgaagacga tgcaccgctc tccgataaat tgggtgacac
     6121 atcactcaag tctgaggcct cgactacaac tacagaacaa accaaggaac acggtaaatt
     6181 agatgacgcg tcatcaaaaa ccgatgacaa ccaagccgaa ccatcatcag acaccaagtc
     6241 gcccgtggac acagttaacc tatctggtca gtcgatagcg ccgcagtata agccgcaggc
     6301 gactgataat gattccgcgg aagcggcaag ctctgtattt gataccaaag aatatcatca
     6361 gcctttggcg ccatccgctg ctaaacatgg actaaaaaaa gtgactagtg tcatcggctt
     6421 gatcatactg ctagttttga tcggtgccgc tatcggttac gcctattacg tttaccgaac
     6481 cggtggattt taaacacaac agctttaccg ccagccttaa gctataattt aaggtaatgt
     6541 ttgatggact taacgagcag caattagagg cggctaagca tactgatggc ccacttttaa
     6601 tcctagccgg tgccggttca ggcaaaacca aaactttgac tcatcgtatc gccaacctga
     6661 tccatagcca aagtgtcgat ccggtcaata ttttagctgt aacttttacc aataaagccg
     6721 ctaaagagat gcgcgagcga ctcggtattc tactgggtgt cgatggtgat cagcgtagct
     6781 ttatgccatg gatgggtact tttcactcta tctgcgttcg tctgctcagg atcgacggtc
     6841 cgcagatcgg tataccatcc aattttgtca tactcgatga atctgatcgc ctaagcttga
     6901 tcaagcaagc gatgaagagc ctgtctatat cggaaaagaa ctatacgccg cgatcaatgg
     6961 ctagtttgat ttcgagtgcc aagaatgaag gactcagccc tgatgagtat gccgctactg
     7021 ctcggatgcc actcaataag accgtagcgg cgatttatcc agcttatgaa agcttaagac
     7081 gcgatgccaa ggcgctggac ttcgacgacc tgctagttga gaccgttcga ctactaaaaa
     7141 cggtggccga cattcgccgt aaatggcagg aacagtttaa ttacgtttta gtcgatgagt
     7201 atcaagatac caataccgtt cagtaccgct tgataaagtt gcttttaaac ggcaagcaca
     7261 acctgtgtgt cgtcggtgac gactggcagt ctatatactc ttggcgcgga gccgacttta
     7321 ctaacatctt gaactttgaa cgggacttca aaaacgtcaa agtcatcaag ctagagaaga
     7381 actaccgctc gaccaagccg attctggaag ccgctcacaa tgttatcacc aaaaaccagc
     7441 aacgaactgc caagaaattg gtcagccagt taggcgatgg tcagccggtc caaatcctac
     7501 acgtgcagag cgaaatacac gaggctgagg cggtagtgac caggataaaa accgcaatcg
     7561 atctccgtct cagacaatac aatgatttcg cagtcttata ccggactaat gctcagagcc
     7621 gggccttaga agaagtgttc atgcgctatg gtctgcccta taagatcatc ggtggcacca
     7681 ggttttatga ccgcgccgaa gtcaaagacg tcatggctta cttaaggctg gtttaccaac
     7741 cggcggatag agccagcttc ttgcgcatag ttaacttacc tgctcgaggt atcggtaaag
     7801 tctcgctggg gcgctttctg gattggcatg actcaatcca gttagatatc gtcgggtgtc
     7861 taaatcaagt cgaactatgt cctggactgc cgactaaagc acttaacgga cttaaagctc
     7921 tcggtcgcat actaacggat ctgttggagc gacacgatga cagtagcttg tttgagttga
     7981 tcaatgccgt gatcaagcga acgggctact tggattatct ggatgacggt acgccgcgtg
     8041 gtgatgatcg agtcgccaac gtaaaggagc tgctgtctga cgccgaacag cggccggata
     8101 ccggcttagc cgagtatctc gaagaagttg ccttggtctc gtcgcaggac agcgaagcta
     8161 gcgaaggcgc ggtgactttg atgacactac atgccgccaa gggacttgaa ttcccggtcg
     8221 tctttatggt cggtatggaa gaagggctat ttccgagcgg ccaatcagtt tacgaagccg
     8281 ataaactcga agaagagagg cggttatgct atgttggcat gacacgcgcc aaagaagagc
     8341 ttttcttgac cacggcaagt tctcgtctaa ccttcggtgc tcgtagctac aatccaccgt
     8401 cacgtttctt agccgacatg gaagtggccg agacctcggc cgcccaagta agcttgccga
     8461 cgccgagtct ggcttcgaac gaaccgagac tggtgcttga tgaagttgat ctgcagattg
     8521 gcgacaaggt caaacactca ttattcgggg tcggtacggt caccgatctg gatggcagca
     8581 acgttgccgt agcatttgcc ggtaaagggg tgaaaaagct aaatacggct tttgcacctc
     8641 tagagaagct ctagcgcaag cgtttgcttt ttgccttagc ccgggctaaa atggatgcag
     8701 attgtgagat ggaatcaaaa cactttttag atgacaatta gcgaacttag ataccttttt
     8761 aagaaagggt ggattattat cgcggctacg ctggccatcg ccttgacctg gacggtccaa
     8821 ggagccttag ctgataccgc ttcgacaccg tcactcggtg agcacctcgt cacgatttat
     8881 gacagtggtc aagaaaaatc actcgtcacc aaggcgccga ctgtcggtga ggccttggaa
     8941 gaagccggtg ttgagctgtc cgacaaagac caggttgaac cagctgtcga tactcccctg
     9001 ctgagcaaga actaccacat caatatctat cgctctaggc tgatacttat agttgacggg
     9061 agtaaacgat tgtcggtagt gacggccgcc cagagtccgg agtctatcat gaaagccgct
     9121 ggcctcgatc tgtatgacga ggatcgggct gaactcaagc cgattgacaa tattctaagc
     9181 ggcggagccg ggttggagtt gactatcgac cgtgccacgg tctttaagtt caacttatac
     9241 ggcaagactt ttgttgatcg gactcaagct gaaacagtag ggcagctatt ggctgataag
     9301 ggcgtgactc ttgggccaaa agacggtgtc actcctagtt cgtccacccc tattgaaacc
     9361 aatatgttag tccatgtttg gcgtaacggc aaacagactg tcacgcaaga acaaaccatc
     9421 aaagcaccag tgaaaaagat ctacgattat gaccggccgc tcggctacaa acaagtcaaa
     9481 gttgctggcc aggatggtat caagcaggtg acctacgaaa tcgttatgaa gggtggccgt
     9541 caagtctcca agaaagtcat agctacggtg gtaactaaga aaccggtaac tgagcaagta
     9601 gtcgtaggtg tcaaatccgg taatcctagt gaaaacgccg ctatcatctg ggcgtactta
     9661 acctcccacg gttttagtcc tgaacagacc gccggtatca tgggtaactt acagcaagaa
     9721 cacggcttca aaacttccgg tgacggtttg gcgcagtgga caggtggtcg aaaggccaat
     9781 ctgatgtcgc gcgacgatcc atattcgctt tatacgcagc ttgacttcat gctggatgag
     9841 ctcgatggca gctacgctga gggggcaata aaatcgacta actctatcga acaagctacg
     9901 gtcatcttcc aggatcatta cgaacgctgt ggtctgtgcg ccgaatcaaa gcgtattagt
     9961 tacgcttaca gcatctataa tcgattcaat taatatgccc gataagtcac tggggcaaca
    10021 ttggctaaaa gacagagaag tccttcggtc catcgtcgac ctagctgaaa tcaagcctac
    10081 cgatacggtc ttggagattg gccctggtct aggtactcta accagcacct tgctggcaaa
    10141 agccggtagt gtgatagcgg ttgagttcga caagcatcta gcgacaaaac tgccggatca
    10201 atttcctggt aagaacctca cggttatcaa ccaagatgtt ttgcaattcg accttggtag
    10261 cctgccgcct ggttatatcg ttgtggctaa tttgccgtat tacataacat caaaaataat
    10321 tcagtatctg agcgaagccg ataattcacc agtaagcgcc gtactgcttg tccaaaaaga
    10381 agtcgccgaa aggttagcgg cgcagccggg tgacctaagt ttgttggctg tcgccacaca
    10441 aatatacttt gaagttagcc tcggtccggt agttggagct gaattgttta cgccaccgcc
    10501 gaaagttgat tcacaggtcg tggtgttgct tcgtcgccaa aggccattgg tcgagcaggc
    10561 tgaacgcggt cgactatttc aattggttaa agccggattc tctaatcggc gtaagaaact
    10621 gcggtctagt ttggcgggcg gtttacgtat gccgaagact gaggtagagc ggttgctggg
    10681 tgatgccggt atcgacgcta accttcgggc tcaggacttg gctattgatg actggctaag
    10741 actggccagg ctttaatcta tgatacatag ccatcagcca actagtttgc cggctgatct
    10801 aatcgttggc ttaagctcga ttgaggatgg agatatgaaa cactctccct atagtgatcc
    10861 agccgcggta ttgatgaatc gtcaagagtt cttgcacagg attggtatcg acatacgaca
    10921 gaccaatcta gtgagaattg attataaatc cgatgacttt tgtcgttatc ggatcgctga
    10981 ggatagcgat aacggtgtca gtatgcaggc gtcgcacgac actgcaccgg ctgacgcctt
    11041 agccacacgt caagctggac aagctctgtt tttgccgatt gccgattgtt gttcacttgt
    11101 actagctgac agtgaaagcc cggcttttat gctcagtcat ttggggcgtc acagcgttag
    11161 ccaattgggc ggacgcaaat cggtggaatt cttggttgat aatcttggcg cgaccttgaa
    11221 ggacatcaag gcctggctcg gccctacggt aggggcggca acttatccgc tatttgattt
    11281 tggcgggcgt tcgctacaaa acgttatcgt cgagcagctt atgacggctg gcttgaagcg
    11341 tgaacaaata gagatttgtc cagtcgacgt agcggaagac gagcgctatt tttcgcacag
    11401 ccagtttaaa aagggcaaaa gaccatacga tggtcggttt gcggtcgtgg ccatgcgtca
    11461 aagctaaacc cttatctgtt acaatactaa ttgactatga caaaaaagag cctatacatc
    11521 accactgcga ttccttatgc taacgctaaa ccgcatatcg gcaatgcgct tgattattta
    11581 ctggccgata tctgggctcg ctatcagcga caacagggca aagaggtccg ctttcaggtg
    11641 ggtaccgatg agcacggcaa taagatagcc caaaaggcta ctgaggccgg caaagaccct
    11701 aaggcctacg tcgacgagat gtataagaat ttcgagcaat tgatgcaagc cgttggcgct
    11761 gattacaccg attttgtccg taccaccgac attcatcatc gagctgccgt ccagtatatc
    11821 tggcagcaat tggagcctta catctataag ggtaaatacg aaggatggta ctgtactggt
    11881 cacgaagctt tctttacaga caaagaggct gccgaaatgg aaggcgtctg cccagatcac
    11941 cagcaaccgc tggagcgaat cagcgaagac aattactact tcaagatgtc ggccttctcg
    12001 gatcagattc gtgaagctat agataagcac aaattggaga tagtcccgag tttcagaaaa
    12061 catgaatttc ttaatctgct agacactggt ctgaatgatg tcagcgtatc gcgaccgaaa
    12121 aagaatctaa catggggcgt tccggtaccg ggcgatgaca accaggtcat gtacgtctgg
    12181 gttgacgccc tgtctaatta tctgacagtg ctaggttatc ctgatgacga tgattggcaa
    12241 aactactggc cggcggacat tcaagtcatc ggtaaagata tcctaaggtt tcacgctgcc
    12301 atctggccgg ctatgttgtt ggctcttggc ctgcccctac ctaagaaatt gttggttcac
    12361 ggctttgtca atgttggcgg cgccaagatg agcaagactg ttggcaatgt ggttgatccg
    12421 atggaaatca tcgataacta tggcgtcgat ggctttcgct attatttctc gcgccacatt
    12481 ccaacactcg atgacggtga ttttacttgg gagaagtttg aaaacgccta caacaatgaa
    12541 ctggccaatg agcttggtaa cttggtctcg cgcgtcggca agatgatcaa caattaccaa
    12601 gccggtgtca taggcgatgc tatcaaatcc gagcacgatg cggcgatcta ccacgacgct
    12661 atgaagaatc tggagtttaa caaagctatg gacgaagtct ggttgatggt gcgcggcctc
    12721 aatcagtatc tggaagaggt taagccttgg caggtagcta agcaacgtga aactgatgcc
    12781 gaagcggagt cacacctcgg tgagatactg gctcatgccg ccggcacctt gctgcaacta
    12841 gcagcgctac ttgatccatt cttgccgact accgccaaag ccatcaatga tattttctca
    12901 accggtgtag tcaaactgcc agatagtgtg atgtttgaaa agaaatatct gcacacgccg
    12961 gatcctaaag ctagtaacaa tgcctagcca agcctcctca aaggaaagcc agccggctga
    13021 actcttcact gatactcatt gccatatcca cgaagctaat tatcccgagg ctgaggcagc
    13081 gcttatgcgg gctcattctg ttggaattga gagcctaatc tgtgtcggca ccgatgagaa
    13141 aacatcggcc gaagctgtgc agtttgcaga gcaccatgac aatgtctggg ctagcgtcgg
    13201 cctgcatcca cacgacgcca aaagcgggat ttcggccatc aaatatctag aaaaacttat
    13261 tgtgactagc ccggcgaaca agctagtggc catcggcgag tgcgggttag attatttcta
    13321 caataactcg cctcgagaaa agcagatcga catgctacac gcccagcttg agttggccga
    13381 aaagtatggt ttggcagtga ttttccatgt cagagaagcg tttgatgatt tctggccgat
    13441 atggcgagag catggcataa agcgcgccgt cctgcatagc tttaccgata accaggctaa
    13501 cttagacaag gccgtagccg aaggattgtt catcggcgtc aacggtatct cgacctttgc
    13561 ccgggataag caagctatgt atgccgccat acctctggaa aatctagttt tggagaccga
    13621 ttctcccttc ttgacgccta taccgcaccg tggtatggta aatgagccag cgttcgtaac
    13681 gcatgtcgcc aaacatttag cgaatctcca gtctatcgat ctctcggagc tctcgcgcgc
    13741 gacacagcgg aacgcgacca aactcttttt taaaacaaac agctaaacct ttaaaagatt
    13801 ccacaattat aactaacggg tggagaggtt ttaatgacag aacagaccca actctatact
    13861 ttgcctggcg gcgctgaaca aagccgcagg ttatttgatg accaatcgaa ccctgagctc
    13921 gtcgaagctc gtaatatagc tcaagcttcg gaagaattca ggggcgactt acgcgaggtt
    13981 cggtcaacca accctgagga tacctttcgg ctggacgaag aggaagagcc gacatctggc
    14041 ccgctatggg accaacaggc tagggaggta aggctgcttg aacaacgcca acaccacgct
    14101 cgaacgacgc atcaagagcg tttaactggt gctatccaag aaatgtcacc tgctcagaac
    14161 atagctgata tcgccgatac gaccattgcg ttatataact ttcgcaccat gaaggcggct
    14221 tgaaagaggt tctagtcatg tcagacctct taaccaaaac catacaattg attgccggtg
    14281 acagtttttc taagcgacca cttaagaaac tgaccgaacg acaattgatt gagctagagt
    14341 cggagatcgg ccgggaactg tttggaccca taccaaaagg ccatcgtcga gagttctttt
    14401 gtctcgacgc tcacacttgg atctggtatg aggaatggcg agaagaagga cagatcaaaa
    14461 gct
//
