LOCUS       DASSCQ010000001         8112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1298913, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000001 DASSCQ010000000
VERSION     DASSCQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298913"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFH05_00005"
     CDS             complement(<1..350)
                     /locus_tag="VFH05_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET6676627.1"
                     /translation="MADRVAQDEAAILSANERDVDALGKSFGSEVSKDRLKAAVARVR
                     MTADDVKEMADRLRVIADQPDPVGAVTGRWERPDGLQVSRVRGSIGVIGIISELSPLV
                     TVESIALCLKSGNV"
     gene            complement(518..1834)
                     /locus_tag="VFH05_00010"
     CDS             complement(518..1834)
                     /locus_tag="VFH05_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6676628.1"
                     /translation="MASRFPTTIASLTGAESEELVPLCWSFLYFFCLLCGYYMLRPVR
                     DEMAIQGGVQHLPWMMTATFVTLLTATPFFGYLSARLPRERLLLSVYAFFGSHLIAFF
                     LMMSSHVAPQWIARLFFVWLSVFNLFVVSVFWSFMADIFTPDQGSRLFSVIAAGGSTG
                     AVLGPLLTTVLTYAVPIPALMLVSTGFLAVCWFCIRALDGWSRQRPARDIDRGGEAIG
                     GGTWAGMRLALSSPYLLGICGYLFCLTTTATFLYLEQTRLIGEQIVSPQGRTRLFAMM
                     DLAVNVLTFVTQFFVTGPMIRRFGLASALVVLPAVSVAGFALVGLMPLLLVFIFFTVV
                     RRVGEYAIAKPAREVLFTVLTREEKYKAKNFIDTAVSRGGDAATGWMVSMVKTLGVGV
                     TQMALVLVPLAGLWAWLGWSLAKQEEDIRIAKPKGNAVYCQKNGML"
     gene            1929..2963
                     /locus_tag="VFH05_00015"
     CDS             1929..2963
                     /locus_tag="VFH05_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET6676629.1"
                     /translation="MRIIDLRSDTVTKPTEAMREAMARAEVGDDVYGEDPTVNRLQEM
                     VAGLLGKKAALFVPSGTMANQLAIRIQTQPGQEVIAESKAHIVRYEQGAAGALAGVQL
                     HWVTGDRGIMGPEHIEAAIRPKDQHSIQTALICLENTHNAGGGTIYPLSTIERIRAVA
                     SAHGVPMHLDGARLFNAVAATTLPPAAYAQHFETVSVCLSKGLGAPAGSLLLTNDPAL
                     IDKARRFRRMYGGAMRQAGILAAAGIYALEHHIGRLKDDHDNATRLARKLQQIPAVTI
                     NPQHVESNIIIFDIIGHRLCPSELVAALRREHVLINAVGGTSFRAVTHLDVSTRQIDA
                     AADAFARILG"
     gene            3044..3265
                     /locus_tag="VFH05_00020"
     CDS             3044..3265
                     /locus_tag="VFH05_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676630.1"
                     /translation="MDTVTFAQISRILELTDSLGLNREWVEIPLSPARPGLVTRLANG
                     KLEILVDADRPFDEWLGSLPAQIQTAQGT"
     gene            3268..4053
                     /locus_tag="VFH05_00025"
     CDS             3268..4053
                     /locus_tag="VFH05_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676631.1"
                     /translation="MDVEGTNTLPTKEELQSELVPLPELPEPPEPPNEPAYEEIVGLA
                     LRHAKGRRGGDLVSVLLVGSGARRAVTAHSDIDLVVLVKGQADGHEIIRVADRMADIR
                     YWGHKDVEDELAYSPRLPSLLRKARILHDHDAIGAKLIERASHRFRQGPPPVNMNEQI
                     RLKAECFHWLGKAQDFADKPATAQYLLMIFFEDFVSAFFRLRGLWLTAPVDVPRFISS
                     RDGVLGDLADHFLVAPTLPERLNCGRDMANLLFKDVPNPARID"
     gene            4128..5021
                     /gene="gnd"
                     /locus_tag="VFH05_00030"
     CDS             4128..5021
                     /gene="gnd"
                     /locus_tag="VFH05_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET6676632.1"
                     /translation="MEIGFIGLGKMGLNMVTRLQQAGHHVTAYDRSPEALAQATTAGC
                     SAATSLADLVQRLKAPRAVWIMVPSGPPTEETVQAVAMLLESGDVVIDGGNTRFHDDV
                     RRAKELAAKNVQYIDAGTSGGIWGLKNGYCLMIGGERDPVNRLAPIFTSLAPPNGWAH
                     VGAAGAGHYVKMVHNGIEYSLMQAYAEGFELMSKSDYRLDLPRIADLWMQGSVVRSWL
                     LELAASALREDPRLESLKGYVQDSGEGRWMIADAIEKDVPVPTLTLALFTRFRSRQTE
                     SFAEKMLAALRNAFGGHAVKR"
     gene            5030..6565
                     /gene="zwf"
                     /locus_tag="VFH05_00035"
     CDS             5030..6565
                     /gene="zwf"
                     /locus_tag="VFH05_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6676633.1"
                     /translation="MPPVKAIDIKPGQEAIPPVEPCTLVIFGGSGDLARRRLIPALYN
                     LLLDGLLPANFVVLGLGRKQMTDEDFRASLREGVLAHSRQELAEEQWRTFAARLFYLS
                     GGNEDAQTYQALKQRAETLERQLGLPGNRIFYLSIPPSSFTAVCEGLEHAGLAVRTAQ
                     PYARIIVEKPVGRDLASAQQINAVTGRVFDESQIFRIDHYLGKETVQNLMVVRFANSI
                     FEPIWNHKYIDHVQITVSEAEGVGTRASYYEEAGALRDMVQNHLLQLLCLVAMEPPYS
                     LDPDVVRNAKMEVLRCLRPITGKDAEIYTVRAQYAAGKAGEKPVPGYRRENGVKPDST
                     TETYVAVKCFVENWRWSGVPFYLRTGKALSRRASEVSVQFKDIPQILFNANPQQPQPA
                     NVLALRIQPEEGLSLRIASRVPGTRTLTHPVEMEFQYSKVFGRPSPEAYERLLLDVMA
                     GDASRFMRRDAVEASWAWITQILEAWQQQGARWLPEYPAGSHGPVEADRLIQQDGRTW
                     RPL"
     gene            complement(6571..6882)
                     /locus_tag="VFH05_00040"
     CDS             complement(6571..6882)
                     /locus_tag="VFH05_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6676634.1"
                     /translation="MIAEISDREFDTVIAQSAVPVLIEFWKPGCGACRALIRQLEQLH
                     IEVAGKVLILTMNVEDNFQIPSELEISSLPALALYRQGHFERFIGGMGTKDEILKQLV
                     L"
     gene            complement(6879..7511)
                     /locus_tag="VFH05_00045"
     CDS             complement(6879..7511)
                     /locus_tag="VFH05_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET6676635.1"
                     /translation="MILCFGDSLTAGFQSPTSEHPTGQETPYGRFLQEWLGPSVEIRI
                     SGICGELTGEMVMRFRRDVIQHRPSHLVLLGGTNDLGWNAQPADIMRNLVKMYELARA
                     SQITPVPVTVPSVRVDVAGAGPDAAAWIDQHLERRLELNGLIAEYAETKGLVWIDLFT
                     ATAEDGTRQLSVPYSNDGLHLTTAGYRLLARLLYDQLFAKLFVPKPGLNL"
     gene            7686..>8112
                     /locus_tag="VFH05_00050"
     CDS             7686..>8112
                     /locus_tag="VFH05_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676636.1"
                     /translation="MSELKSAQDLMAAAMAAKPTVDPEMASVVQLLKSLDRASKNVRT
                     FGHQNIVAKKFFDQFYTELHAHLEQYSVLAFLIQREGLFFKEGCVYNSQAGESSENFA
                     FKLYSDGIREITFHHGIEQEDVLFFFDALWDTGGASGTED"
BASE COUNT         1777 a         2458 c         2305 g         1572 t
ORIGIN      
        1 ctcacattgc cggatttgag acacaaggcg atcgactcga ccgtgaccag cggtgacaat
       61 tcggaaatga tgccgataac cccgatggaa ccccgcacac gcgacacttg aagcccatca
      121 ggacgctccc accgtccagt cacggcgccc accggatcgg gttggtcggc gatgactcga
      181 agccgatcgg ccatttcttt gacatcatcc gccgtcatac gcacgcgggc gacggccgcc
      241 ttcagtcggt ccttactgac ctcacttcca aacgatttgc cgagcgcgtc cacatctctc
      301 tcgttcgccg agagaatggc tgcctcatcc tgggccactc gatcggccat ggctcgcagg
      361 gcccggtcct tgactggacc ggatattacg gaagtcctgc ggacggcatt tctacacacc
      421 ttcaatatct tgtctaagta aagcttaact ggaacttcag gcattcaggc cctcgtgatg
      481 tgcgatgaat taccatcaga cgctcttgag tgccggacta taacatgccg tttttctgac
      541 agtaaacagc atttccctta ggtttcgcga tgcgtatgtc ttcctcctgc ttggcgagcg
      601 accaaccgag ccacgcccac aaaccggcga gcgggaccag caccaaggcc atctgtgtga
      661 ccccgacgcc cagggtttta accatgctga ccatccaacc ggtcgccgca tctccgcctc
      721 gggagaccgc ggtatcgata aagttttttg ccttgtattt ctcttcccgg gtcagcacgg
      781 tgaacaatac ctcccgtgcc ggtttggcaa tcgcatactc tcctacgcga cggacaaccg
      841 taaagaaaat gaacacaagc aacaacggca tgagcccgac cagtgcgaag ccggcgacac
      901 tgacagcggg caatacgacc agcgctgagg ccaacccgaa cctcctgatc atggggccgg
      961 tgacaaaaaa ttgcgtgaca aatgtcagca cattgaccgc aaggtccatc atggcgaaca
     1021 gtctcgttcg accctggggc gagacaatct gttcgccgat cagtctcgtc tgttccagat
     1081 agagaaacgt agccgtcgtg gtgaggcaaa acagatatcc gcagatgcct aggagatagg
     1141 gcgaggaaag ggctaggcgc atccccgccc atgtgcctcc tccgatggct tcgccgcctc
     1201 ggtcgatgtc ccgtgcaggg cgctgtctcg accagccatc gagggctcga atacagaacc
     1261 agcagacggc caggaatcca gtcgacacca gcatgagcgc aggaatcggc acggcatagg
     1321 tcaacaccgt cgtcagcaag ggccctaaca cggcaccggt acttccgcct gccgcaatga
     1381 cgctgaacag ccggctgccc tggtcgggcg taaaaatgtc ggccatgaaa ctccagaaga
     1441 ccgacacgac aaacaggttg aacaccgaca gccagacgaa gaacaggcgc gcgatccact
     1501 gcggcgcgac atgactgctc atcatgagaa aaaacgcgat caagtggctg ccaaaaaacg
     1561 cgtagacgct cagcagtaat cgttcgcgcg gcaagcgggc tgacaaatag ccgaagaatg
     1621 gtgtcgccgt cagcagcgtg acgaaggtcg ccgtcatcat ccagggtaaa tgttggacgc
     1681 cgccctgaat cgccatttca tcgcgaaccg gccgcagcat gtaatacccg cagagcaggc
     1741 agaaaaaata gagaaacgac caacaaagcg gcacgagttc ttccgactcg gcaccggtaa
     1801 gcgaggcgat ggtcgttggg aagcggctgg ccacggcggc atcctgaccg gctcttcgtg
     1861 cgttcgcaag caaaatgcgc ccgagagttt tggacttttt tgctcgaccc cgttatggta
     1921 gggccggtat gcgaatcatc gacttacgca gcgacacggt gaccaaaccg acggaggcga
     1981 tgcgggaggc catggcgcgc gccgaggtcg gcgatgacgt gtatggcgag gaccccaccg
     2041 tcaaccggct gcaggaaatg gtagcggggc tgctcggcaa aaaggctgca ttgttcgtac
     2101 cttcaggtac gatggccaac caattagcca tccgcattca gacccaacca ggccaggaag
     2161 tcatcgccga gagcaaggcg catatcgtac ggtatgaaca gggcgcggcc ggcgctctcg
     2221 ccggcgtcca actacattgg gtgaccggcg atcgcggcat catggggccc gaacacatcg
     2281 aagccgcaat ccgtcccaaa gaccagcaca gtattcaaac cgcgctcatc tgcctggaga
     2341 acactcacaa tgccggcggt gggacgatct atcccctgtc gacgatcgaa cgtatccgtg
     2401 ctgtcgcgtc cgcacacggt gtcccgatgc atttagacgg cgcgagactg ttcaacgccg
     2461 tcgctgccac gaccttgccg ccggcggctt acgcgcaaca ctttgaaacc gtatccgtct
     2521 gcctctcaaa agggttggga gcacccgccg gctctctgct cctcaccaac gatcccgcat
     2581 tgatcgataa agccagacgg tttcgccgca tgtacggcgg cgccatgcgg caggccggca
     2641 tcctcgccgc ggccggcatc tatgcgctcg agcatcatat cgggcggttg aaggacgacc
     2701 acgataatgc gacgcgcctg gcgagaaaac tccagcagat tccagcagtc acgatcaatc
     2761 cgcagcatgt tgagagcaac atcatcatct tcgatatcat cggacaccgg ctgtgtcctt
     2821 ccgagcttgt cgcggcatta cgaagggaac acgtgctgat caacgccgtg ggaggcacaa
     2881 gcttccgggc cgtcacccat ctcgatgtct caaccaggca gatcgacgca gccgccgacg
     2941 cctttgcgcg gattctcggc tgacggcctc cgtcatacga tgcaccctgg cagatttttc
     3001 aggcatcctg ctagaatggc accagtcttg gagacgccat ccgatggaca cagtcacctt
     3061 tgcacagatc agtcggattt tagaattgac cgactcgctt gggctcaacc gcgaatgggt
     3121 ggaaatcccg ttgtctcctg cccgtccggg actggtcacg cgtctcgcca acgggaaact
     3181 ggaaatcctt gtcgacgcgg atcgcccctt tgatgagtgg ctgggatcgc tgccggcaca
     3241 aatccaaacg gcgcaaggaa cgtaatcatg gatgtcgagg gtacgaatac gcttccaacg
     3301 aaggaagaac tgcagtctga gttggttccc ttgccggaac tgcctgagcc acctgagccg
     3361 ccgaatgaac cggcctatga agagatcgta gggctcgcct tgcgccacgc gaaaggccgc
     3421 cgaggcggcg acctcgtgag cgtcctgttg gtcgggtcag gcgccaggcg ggccgtgacg
     3481 gcgcacagtg acattgattt ggtcgtgctc gtgaaaggtc aggcggacgg ccatgaaatc
     3541 atccgtgtcg ccgaccgcat ggcggacatc cggtactggg gacacaaaga cgtcgaagat
     3601 gagctggcct attcaccccg cttaccctcg ttgttgcgca aggcccgcat tcttcacgat
     3661 catgatgcga tcggagccaa actgatcgaa cgggccagtc accgtttccg gcagggccct
     3721 ccacccgtga acatgaacga acaaatccgg ttgaaggcgg aatgtttcca ctggctgggc
     3781 aaggctcaag acttcgcgga caagcccgcg acggcacagt acctcttgat gatctttttc
     3841 gaggatttcg tctctgcatt cttccgtctg cggggcctct ggctgaccgc accggtcgac
     3901 gttccgcggt tcatctcctc acgcgacggc gtcctcggcg atttggcgga ccactttctc
     3961 gtggccccga cgttgcccga gcgcctgaat tgtggaagag acatggccaa tcttttgttt
     4021 aaggacgtgc ccaatccggc ccgcatcgac tgagagggtt cgcttcgctc cgcttctgat
     4081 ttacaacgtg gcatcgatct caactcgcct cctgtagaag gtgtgtcatg gaaatcggat
     4141 tcatcggtct tggaaagatg ggcttgaaca tggtgacccg cttgcagcag gccgggcatc
     4201 acgtgacggc ctacgaccgc tctcccgaag cgctcgcaca ggcgacgacc gccgggtgca
     4261 gtgctgccac ttcgttggcg gatttggtcc aacgactgaa ggccccccgt gccgtgtgga
     4321 ttatggttcc gtctgggcca ccgacggaag agaccgtcca agccgtcgca atgctgctgg
     4381 agtccggcga tgtcgttatc gacggcggca acacccgctt tcacgacgac gttagacgag
     4441 ccaaggagct cgcggcaaag aacgtccagt atatcgacgc agggacgagc ggcggaatct
     4501 ggggactgaa gaacggttat tgcctgatga tcggcggcga gagagacccg gtcaaccgac
     4561 tggcccccat cttcacctcg ctggccccgc cgaacggttg ggcacatgtg ggggccgcgg
     4621 gcgcaggcca ttatgtcaaa atggttcaca atggcataga gtacagcctg atgcaggcct
     4681 acgccgaagg cttcgagctg atgtcaaaaa gcgactatcg cctcgatctt ccacgcatcg
     4741 cggacctttg gatgcagggc agtgtcgtcc gttcctggct cttagaactc gcggcaagcg
     4801 ccttgcgaga ggacccgcga ctggaaagcc tgaaaggcta cgtacaagac tcgggcgaag
     4861 ggcgctggat gatcgccgat gccatcgaga aggacgttcc cgtgccgact ttgaccctcg
     4921 ccctcttcac ccggtttcgc tcgaggcaaa cagaatcctt cgccgagaaa atgctggccg
     4981 ccttgcgcaa cgcctttggc ggtcacgcgg taaagcggta agatcagcca tgccacccgt
     5041 caaagcgatc gacattaagc cgggacaaga agccattcca ccggttgaac cctgtacgct
     5101 ggtcatcttc gggggatccg gcgacctggc ccggcgccgc ttgattccgg cactctacaa
     5161 tctgctgctc gatggtctgc tgcccgcaaa cttcgtcgtg ctcggtctcg ggcgcaaaca
     5221 gatgacggac gaagatttca gggccagcct tcgtgaaggg gttctcgccc attcccgcca
     5281 ggagctggcg gaagaacaat ggcgcacctt cgcggcccgc ctgttttatt tgtccggtgg
     5341 aaacgaggat gcccagacct accaggcgct caagcagcgc gccgaaacgc tcgagcggca
     5401 gttgggccta cccggcaacc gcatttttta tctcagcatc ccaccgagtt cctttaccgc
     5461 cgtgtgcgag ggactcgagc atgcgggact cgccgttcgc accgctcagc cctatgcgcg
     5521 catcattgtg gaaaagccgg tgggccgcga ccttgcctcc gcccagcaga tcaacgccgt
     5581 gaccggtcgc gtattcgacg aatcgcagat tttccggatc gatcactatc tcggaaaaga
     5641 aaccgtccag aacttgatgg tcgtgcgatt cgccaacagt atttttgagc cgatttggaa
     5701 ccacaagtac atcgaccatg tccaaatcac cgtcagcgaa gccgaaggcg tcggcaccag
     5761 agcttcctat tatgaagagg ccggggcgct ccgcgatatg gttcagaacc atctactcca
     5821 gctactctgt ctcgtcgcca tggaaccgcc ctattcgctt gaccccgatg tcgtcagaaa
     5881 cgccaagatg gaagtcctcc gctgcttgcg gccgatcacg gggaaggacg cagagatcta
     5941 cacggtccgt gcccaatatg ccgccggtaa ggcgggagaa aaaccggtgc ccggctatcg
     6001 gcgggaaaac ggcgtgaagc ccgactcgac gacggaaacc tacgtggccg tcaaatgttt
     6061 cgtagagaac tggcgctggt cgggcgtgcc attttatttg agaaccggca aagcactgtc
     6121 gaggcgtgcc agcgaggtgt cggtgcagtt caaggacatt ccacagattc tgttcaacgc
     6181 caatccacag cagccgcagc ccgccaatgt gttggctctg cggatccaac cggaggaagg
     6241 attatcgctg cggattgctt cacgggtgcc cggcacgaga acgctcactc atccggtcga
     6301 gatggagttc caatacagca aggtgttcgg acgtccttcg cccgaagcgt acgaacggct
     6361 gttactcgat gtcatggcgg gtgatgcgtc gcggttcatg cggcgggacg ccgtcgaggc
     6421 atcgtgggcg tggattacac aaattttgga ggcctggcag cagcagggag ccagatggtt
     6481 gccggaatat ccagccggaa gtcacggacc cgtcgaggcg gatcgtctca tacagcagga
     6541 tggccggacc tggcgccctc tttaaggctc tcacaagacc agctgcttga gaatttcatc
     6601 cttcgttccc atcccgccga taaaccgctc gaaatggcct tggcgataca gcgccaaagc
     6661 cggcagcgag ctgatttcca actcggaagg gatctgaaaa ttgtcctcga cgttcatcgt
     6721 gaggatgagc acctttcccg cgacttctat gtgcagctgt tctagttgac ggatgagcgc
     6781 acggcaagcg ccgcagccgg gcttccagaa ttcgattagc accggcaccg ccgactgagc
     6841 gatgacggtg tcgaactcgc gatccgagat ctccgcgatc acaggttcag ccccggctta
     6901 gggacaaaca gcttcgcaaa tagctgatcg tacagtaacc gggccaatag ccggtagccg
     6961 gccgtcgtca ggtgcagccc atcattcgag tacggaaccg acaactggcg tgtgccgtct
     7021 tccgcagtcg ctgtaaacag atcgatccag accaggcctt tggtttcggc atactccgcg
     7081 atcagcccat tcagttccag tcgccgctcg agatgttgat cgatccatgc agcggcatct
     7141 ggtcccgctc cggccacatc cactcgaacc gagggtaccg tcacaggaac gggcgtgatc
     7201 tgtgaggccc gagccaattc atacatcttc accaaattcc gcataatgtc ggctggctgt
     7261 gcgttccagc cgaggtcatt ggttccaccc aacaggacga ggtggctggg tcggtgctgg
     7321 atgacgtcgc ggcggaaccg catcaccatt tcgccggtca attctccaca aattccactg
     7381 atgcgaatct cgacagaggg tcccagccat tcctgcagga aacgcccata gggagtctct
     7441 tgtccagtcg gatgctcgga agtgggagac tgaaatcccg cagtcaaact atcgccgaag
     7501 cataggatca ctcctcctcc tctgggtttt tttctgccct tcgtatgcga cgaaccctca
     7561 cctcgagcga ttgtactgac gtctcgtctt acaggacaac cacgcgcctt ccggacgaat
     7621 gctgagaatt ccaaaacttt tgaaatcctt gacgaaatgc cggcttaccg gtacgactga
     7681 cagtaatgtc ggaactcaag agcgcacagg acttgatggc cgctgcgatg gctgcgaagc
     7741 ccactgtgga ccctgaaatg gcatccgtgg tccaactgct gaaatcgctc gatcgggctt
     7801 caaaaaatgt ccgcacgttc ggccatcaga acatcgtggc caaaaagttt ttcgaccagt
     7861 tctacacgga gttgcacgcc cacctcgagc agtacagcgt cctggcattc ctcatccagc
     7921 gagaaggcct ctttttcaag gaagggtgcg tctataactc ccaggccggc gagtcgagtg
     7981 agaacttcgc gttcaagctc tacagcgacg gcatccgcga gattaccttt catcacggca
     8041 ttgagcagga agacgtgctc ttcttctttg acgcgctgtg ggacacaggc ggtgcctcgg
     8101 gcacggagga cg
//
LOCUS       DASSCQ010000002         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1642737, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000002 DASSCQ010000000
VERSION     DASSCQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642737"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFH05_00055"
     CDS             <1..371
                     /locus_tag="VFH05_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HET6676637.1"
                     /translation="LAKTGQSLSSLVADLPATAVTPEIRVDTPDSIKFELVKRVQDRL
                     AGYAKSSRPIGPNNDVVRDVVTIDGVRAIFEDGWGLIRASNTQPALVLRFEASSADRL
                     SAIRAVVEGELAEVKRSMLS"
     gene            411..1178
                     /locus_tag="VFH05_00060"
     CDS             411..1178
                     /locus_tag="VFH05_00060"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET6676638.1"
                     /translation="MRAILAQCTLACLLLLVDQPAMATDVATAPTQVFHTGERLTYDI
                     SWLNVLAGTAVMEVQPDGAGSRARLVTTAQSRPAISKFFPVDNRVESEFDLSAQLPQH
                     MVFRRREGKNKEDIEYTFHHPEGTVTAVRHGTTETLPIPAGTQDLISCLYYVRNQLPM
                     KPGASLKLNVYHDKRNRQVQVYVENIETVEGVWGKAETAQVVVIMPFKGLFINKGDIR
                     VWFTTDERHIPVRMKAKVIIGSIVADLVDGFSAKTSP"
     gene            1313..2326
                     /gene="fbp"
                     /locus_tag="VFH05_00065"
     CDS             1313..2326
                     /gene="fbp"
                     /locus_tag="VFH05_00065"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HET6676639.1"
                     /translation="MVHHPLTVEQFLMANPPASLGGAGSLTNLLSAIGLVGKTLARDL
                     RIAGLLNMLGVTGDTNVQGESVKKLDQIANETFVEVLQRDGVAGALASEEMEKPVFVA
                     SPGRPSPYMVLFDPLDGSSNTDVNMPLGSIFSIVAHKNADQLPGDNDLLQKGTDQLAA
                     GYFLFGPSTMLVFTTGKGVYGFTLDPNVGEYQLSHPGIKIPSRGKVYAVNEGNYQKWP
                     DGTKRYIDSLKQSDNAAGRPYSSRYSGCLVADVHRLLLGGGIYLYPGEVNKPEGKLRL
                     LYEANPMAMVVEQAGGRASTGTVRILDIEPKKLHQRVPLIIGSTDDVRDAERYLQGAA
                     VRS"
     gene            2514..4031
                     /locus_tag="VFH05_00070"
     CDS             2514..4031
                     /locus_tag="VFH05_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET6676640.1"
                     /translation="MKLKGSGLVPYLLLAFGAVIGVVVASDLGWMSSGSAGPDSRPDG
                     LVYPVATAPQQPVTGGTAKSFVEIAKTVKPAVVNIAASRSGKAGDGPQSSPFDDPFFR
                     RFFGDEFFKRDQPHRERKERGQGSGVIVDPNGLIITNNHVVNKADDIRVILSDKREFK
                     GKLIGTDTKTDVAIVKIEATGLSTIPLADSDQLEVGEFVLAVGSPFGLTQSVTMGIVS
                     AVGRASMGIAEYEDFIQTDAAINPGNSGGALVNIRGELVGINTAIFSQSGGNMGIGFA
                     VPSNLARSIMDQLVKNGKVVRGWLGVAIQELTPELASQFGLADTKGVLVSDVMDDSPA
                     KKAGFERADVITEYDGKPMDSPTHLRNAVAQTPIGKKVSIKVIRDKKPKTIDVTIVEQ
                     PKSLGQAGSEDNGDSTAPTGVLSDLDVRELNEELATRYGLKPNERGVVIVRVKPGSTA
                     DDMGVREGDIVLEVNRKPINSIKSYERAISGLSKDQAVLLLLKRKGQTIYLTLRP"
     gene            4048..>4696
                     /locus_tag="VFH05_00075"
     CDS             4048..>4696
                     /locus_tag="VFH05_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HET6676641.1"
                     /translation="MVSRAIFVLLSALAGMALFIKADGASTTYWMYGFVIGTITGGLI
                     VAGEYALHNLSFGIIVGGTAGLAAGLVLTGLVEWVGGEIFDVQTFLFHIGGLVFLLGF
                     PYLGLVLGARFGKEKLPSPQQKFFELDGNTVCPKVLDTSVIIDGRVADLCETRFLEGT
                     FLVPHFILDELQHIADSSDSLKRARGRRGLDILNKIQKMGDVDVRIIDEDFPHVKE"
BASE COUNT         1051 a         1271 c         1383 g          991 t
ORIGIN      
        1 tattggcgaa gaccggccaa tcgctttcca gcctagtcgc agatctgcct gccacagccg
       61 tcacaccgga aattcgagtg gatactcctg attcgatcaa attcgaactg gtcaaaaggg
      121 tgcaggaccg gctagccggc tatgcgaagt ccagtcggcc tatcggccca aacaacgacg
      181 tcgtccgtga cgtggtgacg atcgacgggg tgcgggccat ctttgaagac ggatggggct
      241 tgatccgggc ctcgaacacg cagccggcat tggtcctccg attcgaagcc agttcagccg
      301 accggttgtc cgcaatccgg gccgtcgttg agggcgaatt ggccgaagtg aagcgctcca
      361 tgctctccta gttccattcc tgccatctcc ccgagacagt catcgttatc atgcgtgcca
      421 tactcgctca atgtaccttg gcctgcctgc tgttgctcgt cgaccagccg gcgatggcta
      481 cagatgtggc gactgcaccg acgcaagtgt ttcacaccgg tgagcggctc acctacgaca
      541 tctcctggtt gaacgtcctg gcagggaccg ccgtcatgga ggtccagccg gatggagctg
      601 gctcacgcgc cagactcgtc acgaccgctc aatcacggcc ggctatcagt aagttttttc
      661 ctgtcgacaa ccgcgtggaa tccgagtttg acctgagcgc ccagctccca caacacatgg
      721 tcttcagacg cagggaaggc aagaataagg aggatattga atataccttc caccaccctg
      781 aggggaccgt gactgccgtc cggcatggga cgaccgaaac ccttccgatt cccgccggaa
      841 ctcaagatct catttcctgt ttgtactacg tacgcaatca actacccatg aaacctgggg
      901 cctctctgaa gctcaatgtc taccacgaca agagaaatcg gcaagtgcaa gtgtatgtcg
      961 agaacatcga gactgtggag ggggtttggg gcaaggcaga gactgcgcaa gtcgttgtga
     1021 tcatgccgtt taagggcctc ttcatcaata agggcgatat tcgcgtctgg ttcacgactg
     1081 acgagcggca cattcccgtc cggatgaagg ccaaggtcat aattggctcc atcgtcgccg
     1141 atttggtcga tggcttttcc gcaaagactt ctccatagaa gaggggcgga gattcattgc
     1201 ccgtgctccc gagacgggct ataatcgctc tcattcattg tcatactgga ggcccacgct
     1261 tcgggttgct ccgaggaagg gtctctttgg tttcatacgg acctgctccc tcatggtgca
     1321 tcacccactc acggtcgaac aatttctcat ggccaatcca ccagcctcgc tcggaggggc
     1381 tggttccctg accaacctgc tttctgccat cggcttggtt gggaagacgc tcgctcgaga
     1441 ccttcggatc gccggcctcc tcaacatgct gggggtgacg ggagacacga atgtccaagg
     1501 ggaatcggtc aagaaattgg atcagatcgc caatgagacg tttgttgaag tgctccagcg
     1561 tgatggtgtg gccggcgcac tggcttccga agaaatggaa aagccggtct tcgtcgcctc
     1621 tccgggaagg ccatccccct acatggtgct gttcgacccc ttggatgggt cgtccaacac
     1681 ggatgtcaat atgccgctag gatcgatatt ttcgattgtg gcgcataaga atgccgatca
     1741 attgccaggc gacaatgact tgctgcagaa gggaaccgac cagctggcag ctggctattt
     1801 cttgttcgga ccgagcacta tgctcgtgtt cacgacaggc aagggcgtct atgggttcac
     1861 actggatccc aacgtcggcg agtatcagct gtcccatccc ggcatcaaga taccctcgcg
     1921 cgggaaagtg tatgccgtca atgaggggaa ttatcaaaag tggcccgatg gaacgaagcg
     1981 gtatatcgac tcgctcaagc agtcggacaa cgctgccggc cggccatata gtagccggta
     2041 ttcaggctgt cttgtcgcgg atgtgcaccg gttgcttttg ggtggcggca tctacttgta
     2101 tcccggcgag gtcaacaagc ccgaagggaa actacggctg ctttatgagg ccaaccccat
     2161 ggcgatggtc gtagaacagg ccggcggcag ggccagtacc gggactgtgc gcattctcga
     2221 cattgagccg aagaagttgc atcaacgagt gcccctgatc atcggcagca ccgacgatgt
     2281 gcgggacgcc gaacggtacc tgcagggagc agcggtacgt tcctagccag gtttgtgttg
     2341 gaaccagcgg aggaccgaca tggccgatca tgcacaagaa attttgagtc actacaccag
     2401 cgacaatccc gaaatcagga ccaacagtct ttccaacgtc cgaaggcgga ggcagtgaac
     2461 tttttgcaga cgacatgggc atctatgccg ggcaagtcca gtaaggaaca gcgatgaaac
     2521 tgaaaggttc aggattggtt ccctatctgt tattggcgtt cggtgcggtc atcggtgtcg
     2581 tcgtggcttc ggatctgggc tggatgtctt cgggcagcgc gggccccgat tcgcgacctg
     2641 acggcctagt ctatcccgta gccaccgcac cgcagcagcc ggtgaccgga ggcactgcca
     2701 aaagttttgt cgagatcgcc aagacggtaa agcctgcagt cgtcaacatc gccgccagcc
     2761 ggagcggcaa ggccggcgac ggtccccaaa gctctccatt cgacgacccg tttttccgaa
     2821 ggttttttgg tgacgagttt tttaagcgtg atcagccgca tcgcgagcgg aaggagcgcg
     2881 ggcagggatc cggagtgatt gtcgacccga acggcttgat catcaccaat aatcacgtcg
     2941 tcaataaagc cgacgacatc cgggtgattc tctcggacaa acgggagttc aagggtaagt
     3001 tgatcgggac cgataccaag actgatgtgg cgatcgtcaa gatcgaagca accggattgt
     3061 caacgatacc cttggcagac tccgatcaac tcgaggtcgg ggagttcgtg ctggcggtcg
     3121 gcagcccatt cggcctgacg cagagtgtta cgatgggaat cgtcagtgcg gtcggacgcg
     3181 ccagcatggg cattgcggag tatgaagatt tcatccaaac cgatgcggcg atcaacccgg
     3241 gaaattcagg cggtgcgttg gtcaacatcc gaggcgagtt agtcggaatc aatacggcaa
     3301 tcttcagcca aagcggtggc aacatgggaa tcggctttgc cgtacccagc aacctggccc
     3361 gttccatcat ggatcaactg gtcaagaacg gcaaggtcgt ccggggctgg ctgggagtcg
     3421 ccatacagga attgactccc gaattggcat cgcaattcgg tcttgcagac accaagggtg
     3481 ttctcgtgag cgacgtgatg gacgacagtc cggccaagaa ggcgggattt gagcgcgccg
     3541 acgtcattac ggaatacgac ggcaaaccca tggattcgcc gacccatctg cgcaacgccg
     3601 tggcacaaac acccatcggc aagaaagtct caatcaaagt catccgagac aagaaaccca
     3661 agacgatcga tgtgacgatc gttgagcagc ctaagtcgtt gggacaggcc ggatcggaag
     3721 acaatggcga ctccactgcg ccgacgggcg tcctctctga cttggatgtg cgcgagttga
     3781 acgaagaatt ggccacccgc tatggcctca agccgaacga acgaggcgtg gtcatcgtcc
     3841 gagtaaagcc gggcagtacg gccgatgata tgggggtgcg ggaaggggac atcgtgctcg
     3901 aagtgaaccg caagccgatc aattccatca agtcctatga gcgggctatc tcggggctct
     3961 caaaagacca agctgtgctc ttgctcttga agcggaaagg ccagacgatt tatttaacgt
     4021 tgcgtcctta acgagaggag tgaaggtatg gtatcgcgag ccatatttgt ccttctcagt
     4081 gcgctggcgg gaatggcgct gttcatcaaa gccgatggcg ccagcaccac ctactggatg
     4141 tatgggttcg tcattggaac gattaccggt gggttaatcg ttgccggcga atacgcctta
     4201 cacaatctgt cattcggcat catagtcggt ggaacggcgg gattggctgc ggggctggta
     4261 ttgaccggct tagtcgagtg ggtgggaggt gagattttcg acgtccagac cttcttgttt
     4321 cacatcggcg gattggtgtt tctcctggga tttccctacc tggggctggt attaggcgcc
     4381 cggttcggaa aggaaaagct ccccagccca cagcagaaat tcttcgaact cgatggcaac
     4441 acggtttgtc cgaaggttct cgatacgagc gtgatcatcg acggccgagt ggcggacctg
     4501 tgtgaaaccc gttttctcga aggaacgttc ctcgtaccgc attttatcct ggacgaactc
     4561 cagcatattg cggattcgtc tgactcgctg aagcgggcca ggggacggcg cgggctcgac
     4621 attctcaaca aaattcagaa aatgggcgac gtggatgtgc ggatcatcga cgaagatttt
     4681 ccacatgtga aagaag
//
LOCUS       DASSCQ010000003        13819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1088832, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000003 DASSCQ010000000
VERSION     DASSCQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088832"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFH05_00080"
     CDS             <1..474
                     /locus_tag="VFH05_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6676642.1"
                     /translation="GGDASIIPTPNPDQLALFKNKKLDAVWTVEPWVSRLERDAGGKV
                     LVEQSNESITVLVSSVKFLKTKRHLAARFIRAHRELTDWIVSHPEEAKQMVRQELAAE
                     TKADVSPELIAQSWKRIVLKNDASLDEYQHFVANAQRAGFIRSLPDLSRLIERVN"
     gene            476..1282
                     /locus_tag="VFH05_00085"
     CDS             476..1282
                     /locus_tag="VFH05_00085"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6676643.1"
                     /translation="MFTTEHSQARPQASKLVLEHVSKWFQTSSLHVHALDDVSLRIAE
                     GEFVCLVGPSGCGKSTLLNIIAGLEHPDRGTVRADGQAVDEPGPHRLMMFQESALFPW
                     LTVLGNVLFGLKLRNGMSAAERREKAEYFLELVGLKRFMHSNVHELSGGMKQRVALAR
                     ALAPNPSVLLMDEPFGALDALTREQLYGDIQRIWSQHRKTIVFVTHNVREAVCLGDRV
                     ILFSPHPGRIREEFAIPLPRPRDINSVDLARFATEIMGVLKGYVQSEVAG"
     gene            1279..2049
                     /locus_tag="VFH05_00090"
     CDS             1279..2049
                     /locus_tag="VFH05_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6676644.1"
                     /translation="MITRWLAASLFFVGLIVVWHLVVKAQIWSPLLLPSPLSVLDYLR
                     SAAEDGTLWEATLVTVRRLLIGYGLGILIGLPLGLLTASSRWCHDTIGILALGLQTLP
                     SVCWVPLALLWFGQSEAAMLFVVVMGTLWSLIIATDNGVRTIPPIFRRAARTMGSTGL
                     HTWTHVILPAALPFLISGMKQGWAFAWRSLMAAEIYVTILSGFGLGQLLHYGRELHAM
                     DQVIGVIIVIVLIGLAVDKSLFAPTERFMYRRWGTERA"
     gene            2106..3626
                     /locus_tag="VFH05_00095"
     CDS             2106..3626
                     /locus_tag="VFH05_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6676645.1"
                     /translation="MNRSLTGWFRHLSISQKLLISFGLILSLLALSLTALLFYLARIN
                     SYVDRHKRITVPAIVTAANMRREAYDVKLALHSPIHTEIARQLAHHASQLTSALNLYK
                     GTHAARTHPILYGMLEEHGRLDLAKQEDRAIEDIAQSLATLTANLPHLQRSEADGLIT
                     RVTDGLDQLMDAHTQIDVEMKHEGDRLVGRARLIALSLALLLGLVIAITYVLAARHVV
                     RPLMTLAATADRVAHHDFSARFPAWPSRDEVGTLAASLTTMLASLREHSTSLLRKTKE
                     LEAFTYSIAHDLKGPLREIEGFSSLLEKHFAEGGDQQVRHHIRVIRHSALRLTHMIDA
                     LLKYSRLEQQELPKQQFNVHDMITNLVNERVSFTQSPKPKVELDLQLDDLYGEPVSIR
                     QALANLLDNAIKFSRFRADPEIRISGRRTQSEYVLSVQDNGIGFDMAQTEKIFGLFER
                     LHGPEDYEGTGVGLAIVRLVMDKHGGRVAVESAPGKGSTFTLMIPIDADSESHRPG"
     gene            3658..4995
                     /locus_tag="VFH05_00100"
     CDS             3658..4995
                     /locus_tag="VFH05_00100"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6676646.1"
                     /translation="MRALIIDDEEYVRLVLGQTLRAEGCDVVLATHGEAGIQLLQTQA
                     FDCVITDLRMPGVDGLAVLKWVSDHQPGVDVMLLTGHGEVKDAVEAMKQGAWDFLIKD
                     TPFDGTAVKAALAKLRTVRALRQENLAARRGGYTRDVIIEGSSPAWKKLRGQIAQAAS
                     SKVPVLIQGETGSGKEVVARLLHDLSSRARGPFLAINCGAMNRELLESELFGHEKGAF
                     TGAVGAKTGLVTTAEGGTLFLDELGEMPTQMQVSLLRFLDRGEYRPVGSTRTLQADVR
                     LVGATNQDINVLVTERRFREDLLYRINAVTVHVPALRERQDDVIMLADHFLHSVRAAG
                     LSPRPLSPGAKTALTNYPWPGNVRELHNVIERLVMSGQGIDPISADEVLAVLPHSQAG
                     LQLQHLSQCSLEDVERLHIQRVLEASGGNKTHAANTLDIDYKTLLAKLKKYGL"
     gene            5057..5665
                     /locus_tag="VFH05_00105"
     CDS             5057..5665
                     /locus_tag="VFH05_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676647.1"
                     /translation="MTRRSPSRLSILLGCSIMIPALALSADKPPQKGSSVKEVKTMSE
                     KDQTALALSAAPAHISKEASVMIYGADGKLTEVRKGTNGFTCIPTVMNLPVPDPICMD
                     GASNQWMTDIMNNAPKPSNTVPGIAYMARGGSHLEKDGKVVMSGDGAKVVKEPPHWMI
                     MWPFDASASKLPTAPNPSGVYVMFEGSPYAHIMVYQDPNKMK"
     gene            5732..6247
                     /locus_tag="VFH05_00110"
     CDS             5732..6247
                     /locus_tag="VFH05_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HET6676648.1"
                     /translation="MKVRDKEEQADTIARILDTALSVPGLHSRVGFDPLVGMLPIVGD
                     LLATMTGATILVIARQLHVPWDMQLRMAYNLLKNGLIGTIPLLGDVYSFFFKSHQLNA
                     ALLIRSVKRGKDGQCLLQTKSLTMQDAAALALLILPTVVLVFAASLWFWNHDASLLNL
                     IYPTPYQSRMK"
     gene            complement(6281..7942)
                     /locus_tag="VFH05_00115"
     CDS             complement(6281..7942)
                     /locus_tag="VFH05_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676649.1"
                     /translation="MTMLWPILLLVLAGCRLIGPLAVHPISVGPIEFDPSTIESIGIS
                     LPILGGDEDYDAVVRVSYRVVGATSWKAALPLQRVRPDMLSRAVPTTFPIAEQFAGSI
                     FNLRPDSTYEVKLTIEDPDQGETTRMGLVRTRAVPRDLPLSPHVVHVDSAETLVSALA
                     QAAPGDVITLAKGHYHGPFTVDRSGSADDPIIIKGRGREATVIESPGADYGVLITGSH
                     IHLQDLTIGLSAWGIKLVNTDEVVIHRTHIRDVKYGINARSGGNRNAYICDNLLEGRG
                     VVWPDTSNRTWDYEGIVVTGTGHVICHNTLAGFGDALGLSQPTVIPNRAIDFYGNDVV
                     WGGDDGIELDYSERNVRAFRNRFGNVAMGISFQPIWGGPVYAFRNVIYNTATAPYKLN
                     QEPSGFFIVHNTSIRSGWAWLQYGELVSNFTFFNNLTIGTENAVYMTPAIEAAAIDYN
                     GWSPDGEFTFDYTWTGFSSLHRESPYERHGRLLTAPLFDQPVTQPTRFESFMPPPLSL
                     TLHASSNAIDAGVPLPNINDDYRGEAPDLGVLERGHGLPQYGVRW"
     gene            8093..9331
                     /locus_tag="VFH05_00120"
     CDS             8093..9331
                     /locus_tag="VFH05_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676650.1"
                     /translation="MTPDQARAQYNFLLTLCIRKAEAFGPLAFAFIKDHELSAIGLTP
                     EEQFNLLVATAEAFAEEPKRYTHKLDCLQKALEILPTTSFFDPELARQLQHDIQRLKA
                     ELDLYSAAMRPERPSPAAGKLAQRIVVETDMPDYYLSIAQQRAAAYYQEKYRLTKESK
                     IAQHFTSGTRRFEPDTVAVQKEFPGACAPFVAARTGAWHVMLPFDLKISRSPEDPLDA
                     ALRIWYAKIGYSFPLRYDMGRLCSYYDDQVLEIDMTDPHLLFVSAAPLRETNLGQVDR
                     PVANDVPFEIALPRAFLEGTNSLGPYTQVGCNIKVWFDASSVSLLVQGAPDLHEYGLE
                     GAAGLLTRTYASEKTAAYAGAGSQKWQEGLSFNFVNMHLQLLPDVTTAVVPAHTPIFS
                     LYPVMARGQYQVTDARTLGH"
     gene            complement(9388..9867)
                     /gene="trmL"
                     /locus_tag="VFH05_00125"
     CDS             complement(9388..9867)
                     /gene="trmL"
                     /locus_tag="VFH05_00125"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HET6676651.1"
                     /translation="MFDVILYQPEIPPNTGNIIRLCANTGVRLHLVKPLGFSLDDRQM
                     RRAGLDYHEYATVKVHEDWASCAADFSGKRIFAVSTKGSQRYDTNRYRVDDVFLFGPE
                     TRGLPADLLMQFPPERRIRLPMLAESRSLNLSNAAAVVIYEAWRQVDFADKRTRCQP"
     gene            10042..11367
                     /locus_tag="VFH05_00130"
     CDS             10042..11367
                     /locus_tag="VFH05_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676652.1"
                     /translation="MDSVWRQRLAAITRTRLRPLLLTTAYVAACLLFLTSPGWADEEK
                     PTPLAGEAFDTEVLGEPVQVAPRDRKSVTAASFGIQYLPDGPSFYQVLPFGALYVWRN
                     SDDMRQRFRGTFSGPVNDLTMNVGSRSSTGWEFRFTLNTMIIPLGRSEYVEGQIIRDV
                     EVEWSYVFGGFGLAYRRLLDPGHQDNALELSLTYEPGFRWFKGTSKTSPNFIVPEDTY
                     EGRVHARVRRDALERNLMELPHRGYAFGGDFMYGHRSNWSTWGGGVFGLSNERTYLMG
                     SVFALAATGVPFVSSERHRLVASVYAGLGQNLDRFSTFRLPGRPTGYEWEALALPILP
                     SVAFNELFPRRYGIAHLEYRYEPIFFVFPYIRGSWGLVEQPRFQPDGSIRYKMDAMPA
                     LSGGVVTGAPWRSQVEINYSYNFGIYSDHGGGPPLPGRHGFFVFWSKEL"
     gene            11464..13218
                     /locus_tag="VFH05_00135"
     CDS             11464..13218
                     /locus_tag="VFH05_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676653.1"
                     /translation="MPRAYPRHPLFCSGLLIGFSVLCGWMFVAPGILAEELQGASASD
                     PRIIERAKTDWESGAITPALETLDQGIEAHPDAITLHKLRGDILATSRASTEALQAYE
                     TVLGMQPSALDVRWAKWSLLVRSGQGEQSVEELRRIASVDTHNPLIHLRLARELRKLD
                     HLEASLQSYKKAVELAPDMHGWRLAMARARFDVLDYEGAVNDVQYVLDHIPPGSPLEL
                     PANTLATVFHGHSQDRGRRFDPVFGSKDITPDQLKQWALMRADAWRMFVAGRYYEAEP
                     MYRKLLELNPRDPSAAHQFGLTLMQLGKCDEALKVFGKVSDLDPTEDQYNDIVFRMGQ
                     CLVELERWEEAFMHFRTLYDQAVQFEIQNKDNALPPGMRVLDKKKLLRWLERVKPHIP
                     AELANLDNGPASDVTSMPDAGEAPAPTKEELDAALDRLKPERALDTGGVLVGRDADFS
                     WFRFVIPASKVMRDDLPTGQHDFIPLNPGDTFLTTQQDIYLVFRLVSDSYDAVPLATQ
                     CFLETTESTGHSGMVAQDHVLMAMSDQSGYFTLAPPEHGWTPGLYRCGLFAGERTSAY
                     TQADEVRFRVVQSNSSSS"
     gene            13382..>13819
                     /locus_tag="VFH05_00140"
     CDS             13382..>13819
                     /locus_tag="VFH05_00140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET6676654.1"
                     /translation="MTHRLYYAVMLLVVFALQTTGCATNPYTKRWQLLMVPQSYEANL
                     GAQAYQDVLSSPKVKISDNPSEVTPVQRVASRIIEAAKKSKYADSAKDFKWEVAVIKD
                     DGTKNAFALPGGKIAVYTGIFPVADNESGLAAIMGHEVVHALAR"
BASE COUNT         2951 a         4016 c         3904 g         2948 t
ORIGIN      
        1 ggcggcgacg catcgatcat cccgacgccc aacccggatc aactcgcgtt attcaagaat
       61 aagaagctcg acgcggtctg gacggtcgaa ccttgggtct cgcgtttgga gcgggacgcc
      121 ggaggcaagg tgctcgtcga acaatccaac gagagcatca cggtcctggt ttcgagtgta
      181 aaattcttga agacgaaacg tcatctggct gccaggttca ttcgtgcgca ccgtgagtta
      241 acggattgga ttgtctcgca tccggaagaa gccaaacaga tggtgcgaca ggagctcgcc
      301 gccgaaacca aagccgacgt atcaccggag ttgattgcgc agtcatggaa acgtatcgtg
      361 ctcaagaacg acgcatccct ggatgaatat caacatttcg tggctaatgc gcagcgtgcg
      421 ggttttatcc ggtccctccc cgatttatcg cgcctcatcg aaagggtcaa ttgacatgtt
      481 cacgaccgag cattcccagg cacgccccca ggcatcgaag ctcgtccttg aacatgtctc
      541 caagtggttt cagaccagct ccctgcatgt gcatgccctc gatgacgtgt cgctgcggat
      601 tgcagaaggg gaatttgtct gcctcgtcgg ccccagcggg tgtggaaagt cgaccctgct
      661 caacatcatt gccggcttgg aacatcctga tcgtgggact gtccgggccg atggccaggc
      721 cgtcgacgaa cccggacctc atcgtttgat gatgtttcaa gaatcggccc tgtttccatg
      781 gcttacggtg ctgggcaatg tcctgtttgg attgaagctc cgcaacggca tgagcgcagc
      841 cgagcgccgg gagaaagcgg aatatttttt agaactcgtt ggactcaagc gattcatgca
      901 ttcgaatgtt catgagctct cgggcggcat gaagcaacgc gtcgccttgg cgcgggccct
      961 cgcaccgaat ccgagcgtcc tactgatgga cgaaccgttc ggtgcattgg atgccctgac
     1021 acgggaacaa ctctacggcg acattcaacg gatctggagt caacaccgca aaaccatcgt
     1081 cttcgtcacc cacaatgtcc gcgaagcggt ctgtctcgga gaccgcgtca tccttttctc
     1141 tccccacccc gggcgcattc gtgaggaatt tgccatcccc cttcccaggc cgcgcgacat
     1201 caacagtgtg gatctggcgc ggttcgcgac cgagattatg ggagtcttga aaggctacgt
     1261 ccaatcggag gtggccggtt gatcacacga tggttggctg catcgttgtt ttttgtgggc
     1321 ttgatcgtgg tctggcatct ggtggttaag gctcagatct ggtcaccgct gttactgcct
     1381 tctcctctga gcgtcctcga ctatctgcga agtgctgcag aagacggcac tctctgggaa
     1441 gccacgttgg tcaccgtacg acggctgctc atcggctatg gattgggcat tctcatcgga
     1501 ctacccctcg gactcctgac cgcctcatcg cgatggtgcc atgacacgat cggtattctc
     1561 gcgcttgggc tccaaacgct ccccagcgtg tgttgggtgc cgctggctct gctctggttt
     1621 gggcagagcg aggcggccat gctgtttgtc gtggtgatgg ggacgctgtg gtcactgatc
     1681 atcgcgacag acaacggtgt acggaccatt cctccgatct tccgtcgggc ggcccgcacc
     1741 atgggttcga ccggcttgca cacgtggacc catgtgatcc ttccggcagc ccttccattt
     1801 ttgatcagcg ggatgaaaca aggctgggcg tttgcctggc gttcgctgat ggcggcggaa
     1861 atctacgtga ccattctgag cggcttcggt ctcggtcaac ttctccacta tggccgggaa
     1921 ttgcatgcca tggaccaggt catcggcgtc attatcgtca tcgtgctgat cggcctcgcg
     1981 gttgacaagt cactgttcgc ccccaccgaa cgattcatgt accggcgatg ggggaccgaa
     2041 cgcgcgtaac ggacgcgtgc ttcctctcac tggcacccgc atggtgccta tggtaccgta
     2101 gcgccatgaa ccgctctctg acggggtggt ttcgccacct gtcgatcagt caaaaacttc
     2161 tcatctcgtt cggattgatc ctgagcctcc tggcgttaag cctcacggcg ctgctctttt
     2221 atctggcccg tatcaatagt tatgtggacc ggcacaagcg aattacggtt cccgccatcg
     2281 tcaccgccgc caacatgcgg cgggaggcct acgatgtgaa gctggcttta cactccccta
     2341 ttcataccga gatcgccagg cagcttgcac accatgcttc ccagctcacg agcgcactca
     2401 atctgtataa aggaacgcac gccgcccgta cccatcccat tttgtatggc atgctggagg
     2461 agcatggccg cctcgatctg gcgaaacagg aggatcgggc gatcgaagac attgcccaat
     2521 cccttgccac attgactgcg aatctgcccc atcttcagag gtcggaagcc gacggcctga
     2581 tcacgcgcgt cacagatggt ctcgaccaac tgatggacgc tcacacgcag attgatgtgg
     2641 agatgaagca tgaaggcgat cgactggtcg gccgcgcccg cttgattgct ctttcgctgg
     2701 ctctcttact cggacttgtc atcgccatca cctatgtgct cgcggcaaga cacgtcgtcc
     2761 ggcctctgat gacgctggcc gccaccgcgg accgggtggc ccatcacgat ttctccgccc
     2821 gatttcccgc gtggccgagc cgcgacgaag tcgggactct cgcagcctcc ctgacgacga
     2881 tgctggccag tctgcgggag cacagcacat cgctcctgcg caaaacaaaa gaattggaag
     2941 ccttcaccta ttcgatcgcc catgatctaa agggaccatt gcgtgaaatt gaaggttttt
     3001 cctctctgtt ggaaaagcac tttgccgaag gcggcgatca gcaagtgagg caccacatca
     3061 gggtcatcag acactcagcg ctccgcctca cgcacatgat cgatgccctc ctcaagtatt
     3121 cgcgtctaga gcagcaggaa ttgccgaagc aacagtttaa cgttcacgac atgatcacga
     3181 acctggtgaa cgagcgagtc agctttacgc agagcccgaa accgaaagtg gagctcgacc
     3241 tgcagctgga cgatctgtac ggggagccgg tcagcatccg tcaagcgctc gcgaacctgc
     3301 tggataacgc catcaagttc tcacgtttcc gggccgaccc ggagatacga atcagcggca
     3361 ggaggaccca gagcgagtat gtcttatctg tacaggataa cggcatcggt ttcgacatgg
     3421 ctcagaccga gaagatcttc gggctgttcg aacgcttgca cggtccggaa gactatgaag
     3481 gaaccggagt aggcctggcg atcgtccgac tcgtcatgga caaacatgga ggccgggttg
     3541 cggtcgaatc tgcccccggc aagggaagca cctttacact catgattcca attgacgctg
     3601 attccgaaag tcataggccc gggtagcggt tgcatcaggc ggagacacaa ttcgagcatg
     3661 cgcgcgctga ttatcgacga cgaagaatat gtgcggttgg tcttggggca gacgcttcgt
     3721 gcggaaggat gtgacgtcgt cctcgccacg cacggtgagg cagggatcca attgcttcaa
     3781 actcaagcct ttgactgtgt gattaccgat ctacggatgc ctggtgttga cggactggcc
     3841 gtcttgaagt gggtctccga tcaccaaccc ggggtcgacg taatgttgtt gacagggcac
     3901 ggggaggtga aagatgccgt tgaggcaatg aagcaaggcg catgggattt tctcatcaag
     3961 gacacgccgt ttgacggcac tgcggtcaaa gcggcgttag ccaaactgag aaccgtacga
     4021 gccttacggc aggaaaacct cgctgcgcgc cgcggcggat atacacggga cgtcattatt
     4081 gaaggatcaa gcccggcatg gaagaagctg cgaggccaga ttgcacaagc cgcctcatca
     4141 aaagtccccg tgctcatcca gggagaaacc ggctccggca aggaagtggt ggcgcggcta
     4201 cttcatgatc tgagcagcag agcgaggggg ccatttcttg ccatcaattg cggagcgatg
     4261 aaccgcgaac tcctcgagag cgaactgttc ggtcatgaaa agggcgcgtt caccggcgcc
     4321 gtcggtgcaa agaccggctt ggtcacgacg gcggaaggag gcaccctctt tcttgatgaa
     4381 ttgggagaga tgccaaccca gatgcaggtc agtcttctcc ggtttttgga tcgaggcgaa
     4441 tatcgaccgg tcggcagcac ccggaccctg caagcggacg tccgtcttgt gggagcgacc
     4501 aaccaggaca tcaacgtcct cgtcactgaa cgccgctttc gagaggatct gctgtatcgt
     4561 atcaatgcgg tcaccgtgca cgttcctgcg cttcgtgaac gccaggacga tgtgatcatg
     4621 ctggcagatc atttcctgca cagcgtccgc gcggcgggac tgtctcctcg accgctctcc
     4681 ccgggcgcaa aaaccgcact caccaattat ccttggccgg gcaatgtccg ggaattgcac
     4741 aatgtcatcg agcggttggt catgtccggg cagggcatcg acccgatttc ggccgacgaa
     4801 gtcttagccg tcttgccaca ctcacaagca ggattacagc tccaacacct ttcacaatgc
     4861 tcactggaag acgtcgaacg attacacatc cagcgggtgt tggaagcgag cgggggcaac
     4921 aaaacgcatg ccgccaacac cttggacatt gactataaaa cgcttcttgc gaaactcaaa
     4981 aaatacggtc tatgattcgc ggttcgtctc agtcaaatcg attaacggga agttcacttt
     5041 ctcaaggagg atcgccatga cccgtcggag tccgagtcgt ctcagtatcc tcttaggctg
     5101 ttcgatcatg atacctgccc tggcgctctc agccgacaag ccgcctcaaa aaggatcgtc
     5161 ggtcaaagaa gtcaagacga tgtccgaaaa ggaccagact gctctcgctt tgagtgcagc
     5221 accggcgcac atttccaagg aggccagcgt gatgatttat ggcgccgacg gcaaactcac
     5281 agaagtaaga aagggcacga atggctttac atgtatcccg accgtcatga acctgcccgt
     5341 ccctgatccc atttgcatgg acggcgccag caaccaatgg atgactgaca tcatgaacaa
     5401 cgcgcctaaa ccgtccaata ccgtcccggg gatcgcctat atggcacggg gaggatcgca
     5461 cttggaaaaa gacggcaaag tcgtgatgtc cggtgatggc gccaaagtgg tcaaggaacc
     5521 gccgcattgg atgatcatgt ggccgtttga tgcctcagcc tcgaagctgc cgaccgcgcc
     5581 caatccgtcc ggagtgtacg tcatgtttga gggaagtccc tacgcgcaca tcatggtgta
     5641 tcaggatccg aacaaaatga agtgatcact tccggcgacg ttggctgctg gactgacaac
     5701 accctagccg ccaggagcgt atgctgactg tgtgaaggtt cgagacaaag aagaacaagc
     5761 cgacaccatc gcgcgcatac ttgacactgc gctgtccgtc ccgggattgc attctcgagt
     5821 cggcttcgat ccgttggtcg gcatgcttcc gatcgttggc gatctgctcg cgacaatgac
     5881 cggcgcgacg atcctggtta tcgcccggca gttgcatgtc ccgtgggaca tgcaactgcg
     5941 aatggcttac aatctgctga aaaacgggct gattggaacg ataccgttgc tcggcgacgt
     6001 gtattcattt ttcttcaaga gccatcagct caatgccgcg ttactgattc gttccgtgaa
     6061 acgaggtaaa gacgggcaat gcctgttgca aacgaaatcc ctgacaatgc aagacgccgc
     6121 cgccttggca ttgctcatcc ttccaaccgt tgtcctggtg ttcgctgcca gcctgtggtt
     6181 ctggaatcat gatgcctcgc tcttgaatct catttatccg acaccttatc aaagccggat
     6241 gaaatagtcc gccgcagatt ggcgagcagc gtccgtctca ctaccagcgg accccgtact
     6301 gcggcaagcc atggccacgt tcaaggacac ccaagtccgg tgcttcaccg cgataatcgt
     6361 cattgatatt ggggaggggc actccggcat cgatggcatt acttgaggca tgcaacgtca
     6421 gggacaaagg tggtggcatg aaggattcga atcgggtcgg ctgggtgacc ggctgatcga
     6481 agaggggcgc tgtgagcagc cttccatgtc gctcataagg agactcgcga tgcaaggagg
     6541 aaaatcctgt ccatgtgtaa tcgaacgtga attctccgtc aggactccat ccgttgtagt
     6601 cgatggccgc cgcctcaata gcaggcgtca tgtagacggc gttttcggtg ccgatggtca
     6661 ggttgttgaa gaaggtaaaa tttgagacga gttctccata ttgcaaccat gcccaaccgg
     6721 aacggataga cgtgttgtgc acaatgaaga agccgctcgg ctcctgattc aatttgtagg
     6781 gcgccgttgc tgtgttatag atgacgttgc gaaacgcata caccggtcct ccccagatgg
     6841 gttggaagct gatgcccatt gccacgttcc cgaaccgatt cctgaacgcg cgcacgttcc
     6901 gttcgctata gtccaactcg attccgtcgt cgccgcccca gacgacgtcg ttgccataga
     6961 aatcgatcgc gcgattcggt atcaccgtgg gctgtgagag gcccagggca tcaccgaatc
     7021 ccgcaagcgt attgtggcag atcacatgtc ccgtgccggt cacgacaatc ccctcgtaat
     7081 cccacgttcg attactcgta tccggccaca cgactcccct tccttccaag aggttgtcgc
     7141 agatatatgc atttcggttg ccgccgcttc gggcgttgat accgtacttc acatctctga
     7201 tgtgcgtccg atgaatgacc acctcgtcag tgttcacgag tttgatcccc caagcgctga
     7261 gtccgatcgt cagatcctgg agatggatgt gagaaccggt aataaggaca ccatagtccg
     7321 ctcccggcga ttcaatgacg gttgcttcgc gaccgcggcc tttgatgatg atcggatcat
     7381 cggcgctacc actccgatcc accgtgaagg gaccgtggta gtggcccttg gcgagcgtga
     7441 tgacatcacc gggcgcagct tgcgcgaggg cggacaccag cgtctcggct gaatcgacgt
     7501 gcacaacatg aggcgagagt ggaaggtccc gaggcaccgc acgcgtccgc acaaggccca
     7561 ttctggtcgt ctctccttgg tccggatctt cgatcgtcag cttaacctca taggtagaat
     7621 cgggacggag attgaagatg ctgccggcaa actgctccgc gatgggaaag gtagtgggga
     7681 ccgcccggga gagcatatcc ggtcgcacgc gttgtagtgg cagcgccgcc ttccaactgg
     7741 tggcaccaac cacgcgatag gacacccgga ccacggcatc gtagtcttcg tcgcctccga
     7801 gaatcggcag actgattcct atagactcga tcgtcgacgg atcaaattcg atgggaccga
     7861 cagagatggg atgaactgcc aaggggccga tcaacctaca gcctgccagc acgaggagaa
     7921 gaatcggcca cagcattgtc atgggacgga aggatgaggg tgaagccgct gttattctac
     7981 tggcgagatc cctaacgagc cggacagcat gactaggtgc cagtctgaaa agagtttgac
     8041 ggatcacgct gaccttgctc ccttcaaggc gagaccgtta tgatggccga ccatgacgcc
     8101 ggatcaagcg cgcgctcaat acaattttct gttaaccctg tgcatccgta aagctgaggc
     8161 attcggcccg ttggcttttg ccttcatcaa agatcacgag ctgagcgcca tcggtctcac
     8221 gccggaagag caatttaatc tgctcgtcgc gacggccgaa gcgtttgccg aagaaccgaa
     8281 gcgctacacg cacaaattgg attgcctgca aaaagcgctc gagattcttc ccactacgag
     8341 tttttttgac ccggagctcg ctcgacagct ccagcacgat attcagcgct tgaaggccga
     8401 gctcgatctg tattcggctg ccatgagacc ggaacgtccg agccccgccg ccgggaagct
     8461 ggctcagcgc atcgtagtcg aaaccgacat gcccgactat tacttatcca tcgcgcaaca
     8521 gcgggcggcg gcctattatc aggagaaata tcgcctgacg aaagaatcga agatcgctca
     8581 gcacttcacc tctggcacgc gacgattcga accggacacc gtggccgtgc agaaggagtt
     8641 ccctggtgcc tgtgcgccgt tcgttgcggc tcgaacgggc gcctggcatg tcatgttgcc
     8701 gttcgacctt aagatcagcc gatcacccga agatcctttg gacgccgccc tgcgcatttg
     8761 gtacgccaag atcggatatt ctttcccgtt acgctatgac atgggacgac tgtgcagcta
     8821 ctatgatgat caagtccttg aaattgacat gacggatcca catttgctct tcgtctctgc
     8881 ggcgccgctg agagaaacca acctaggcca ggttgatcga ccagtggcaa acgatgtgcc
     8941 gtttgagatc gcgttgcctc gtgcattcct tgaggggacc aactcgctcg gtccgtacac
     9001 gcaagtcggc tgcaacatca aggtctggtt tgatgccagt tcggtcagcc tgctcgtgca
     9061 gggggcaccg gatctgcatg agtacggctt agaaggtgct gccggattac tgactcgaac
     9121 ctatgcatca gaaaaaacgg ctgcctatgc cggcgccggc agtcagaaat ggcaagaggg
     9181 actgagcttc aatttcgtga acatgcatct ccagttgctt ccggatgtga ccacagccgt
     9241 ggtccccgcc cataccccga ttttttccct ctatcctgtg atggctagag gacagtatca
     9301 ggtgacggac gcccgtactc tcggtcactg aaccatgagt ctgatcgggt acccccgctt
     9361 tattgcgagc gagcagggca cttcgattca cggctggcat cgagtacgct tgtccgcaaa
     9421 atccacctga cgccaggcct cgtagatgac caccgctgcg gcgttggaga gattgaggct
     9481 ccgactttcc gcgagcatcg gcagccggat ccgtcgttca ggaggaaatt gcatcaacag
     9541 gtcggccggc aatccccggg tctcaggacc gaacagaaag acatcgtcta cacgataccg
     9601 gttggtgtca tagcgttggg agcccttggt cgagaccgcg aagatccgtt tgccgctgaa
     9661 atccgcggca cagctcgccc agtcctcatg aaccttcacc gtggcatatt cgtgatagtc
     9721 caaccccgcg cgccgcatct ggcggtcgtc caatgaaaat ccgagcggtt tcacgaggtg
     9781 tagccgaaca ccagtgttcg cgcagagccg gatgatgttc cccgtgttgg gcggaatctc
     9841 aggctggtac aaaatgacgt caaacatgac agtagctccc cctgttatct ccgctcgccc
     9901 tagcccgatg gaagattgtt cgttcgactg agcctcactg gaactcgctg gattcttgag
     9961 ttaatttgca gcctcctccg cccttctaca ctagggccct ccctagtgtc tccctgtcgc
    10021 aaagtcgtaa atgctgaaaa catggactcg gtctggagac agcgcctggc ggccatcaca
    10081 cgcaccaggc taaggccttt gctgctcacc acagcctacg tggcagcctg cctgctgttc
    10141 ctcaccagcc ctgggtgggc ggacgaagag aaaccgacac cgttagccgg agaagccttc
    10201 gacactgaag tgctcggtga accggtccaa gtcgcgcctc gcgatcgaaa aagtgtcacg
    10261 gcagccagtt tcggcattca atacctccct gacggcccga gtttttatca agtcctaccg
    10321 ttcggagcct tgtacgtctg gcgcaattcg gacgatatga ggcaacggtt tcgaggcacg
    10381 ttttccggtc ctgtgaacga tttaaccatg aacgtgggat ctcgatcatc caccggttgg
    10441 gaattccgct tcacgttgaa tacgatgatc attccgctcg gccgatccga gtacgtggag
    10501 ggtcaaatca ttcgggatgt ggaggtggaa tggagttatg tcttcggagg attcggcctg
    10561 gcctatcgaa ggctgctcga ccccggtcac caggataacg cgttggagct gtcattgacg
    10621 tacgaaccgg gctttcgttg gttcaagggt acgagcaaga cgtctccaaa cttcattgtc
    10681 ccggaggata cgtacgaagg acgcgtgcat gcacgcgtcc gccgagatgc cctcgagcgc
    10741 aatttaatgg agctcccgca tcggggctat gccttcggcg gcgatttcat gtatgggcac
    10801 cggtcaaact ggagcacgtg gggtgggggc gtattcggtc tttccaacga acggacatac
    10861 ctcatgggaa gcgtgtttgc gcttgccgcc acgggcgtcc cctttgtcag cagcgagcgg
    10921 cacaggctgg tcgcctccgt ctatgccggt cttgggcaaa atctcgaccg gttttccacc
    10981 tttcgcttgc ctggtcggcc cacaggttat gaatgggaag cgctcgccct gcccatcttg
    11041 ccgagtgtcg ccttcaatga actgtttccg cggcgatacg gcatcgcgca tctcgagtac
    11101 cgttatgagc cgatattctt cgtgttcccc tacattcgag gatcatgggg cctcgtcgag
    11161 caaccacggt tccagccgga cggatctatc cgctataaga tggatgcgat gcccgcccta
    11221 agcggcggtg tcgtgaccgg agcgccctgg cgctcacaag tggaaatcaa ttacagctac
    11281 aacttcggca tttacagcga ccacggtggt ggcccaccgt tgccaggccg tcacgggttt
    11341 ttcgtgttct ggtccaagga actctgacca gcatgacggt gctcggctta ggtgtgcgag
    11401 caagggaagc ctgcgatata atgaagccag gccatgatag ccttgcaacc ctggagactc
    11461 tgcatgcctc gtgcatatcc ccgacacccg ctattctgct ccgggcttct catcggcttt
    11521 tccgttttgt gcgggtggat gtttgttgcg ccgggtattc tcgctgagga gcttcagggg
    11581 gcgtcagcgt cagatccccg catcatcgaa cgcgctaaaa cggattggga aagcggcgcc
    11641 atcacgcccg cgctggagac gctggatcaa ggcattgaag cgcacccgga cgccatcaca
    11701 ctccacaaac tgcgcggcga catcctggcg acgtcgcgcg cttcgacgga agcgctccaa
    11761 gcctatgaga cagtcctcgg gatgcaacct agtgccctgg acgtccgatg ggccaagtgg
    11821 agtctgctcg ttcggtcagg acaaggggaa cagtcggtag aggaattacg ccgcattgcc
    11881 tccgtcgaca cgcacaatcc attgatccac ttgcgtctcg cgcgggagct ccgaaagctc
    11941 gatcacctcg aggcctcact ccagtcgtac aagaaagcgg tcgaactcgc gccggacatg
    12001 cacgggtggc ggctcgcgat ggcccgcgca cggttcgacg tattggacta tgaaggcgcc
    12061 gtcaatgacg tccaatacgt cctcgatcat atcccgcccg gttcgcccct ggagttgccg
    12121 gccaatacct tggccacagt gtttcacggc cactcgcaag accgaggacg acgattcgat
    12181 ccggtgtttg gctccaagga catcacaccc gaccagttga agcaatgggc gctcatgcgc
    12241 gcggacgcgt ggagaatgtt tgtggccggc cgttattacg aagcggagcc gatgtatcgg
    12301 aagttattgg aattgaatcc acgcgacccg tccgccgctc accaattcgg cttgactctc
    12361 atgcaactgg gaaaatgtga cgaagcgctc aaggtgttcg gcaaggtgtc ggacctcgat
    12421 ccgaccgaag accagtacaa cgatatcgtc tttcggatgg gtcaatgctt ggtggaactc
    12481 gaacggtggg aagaggcgtt catgcacttc cgcaccctgt atgatcaagc cgtgcagttc
    12541 gagatacaaa acaaagacaa tgcgctgcct cccggcatgc gggtgctgga caagaagaag
    12601 ctcctgcgat ggctcgagcg tgtgaagccg cacatccctg ccgaactggc caacctcgac
    12661 aatggaccgg cgtccgacgt gacttcgatg ccggacgccg gtgaggcccc agccccgacc
    12721 aaggaagagc tcgatgccgc gttggaccgg ctcaaacccg agcgtgcctt ggataccggc
    12781 ggcgtgctgg ttggccgcga cgcggatttc agctggtttc gctttgtgat tcccgcaagc
    12841 aaagtgatgc gcgacgacct tccgactggc cagcatgatt tcatcccgct gaatccgggc
    12901 gatacgtttc tcacgaccca gcaagacatc tatctcgtgt ttcgtctcgt ctccgattca
    12961 tatgatgccg taccgcttgc cacccaatgc tttcttgaaa cgaccgagtc aaccggacat
    13021 tcaggtatgg tcgcccagga tcatgtgctg atggccatga gcgaccagtc ggggtacttt
    13081 acactcgctc ctccggagca cgggtggacg ccgggcctct accgctgtgg gctctttgcc
    13141 ggtgaacgca catctgccta tactcaagcg gacgaagtgc gcttccgcgt cgttcagtcg
    13201 aactcatcat catcctaatc gctcggtcac gggctccctc cgccgcgggt atcccggtgg
    13261 cacagaagga cctagcaggt gaaccgggcc gcgagattgc tttccctagg ggatgtccta
    13321 atcactgccc gtccgccaag ccttttagaa tatctctgta tcctctacca ggagggctga
    13381 catgacgcat cggctatatt atgcggtgat gctcctagtc gtttttgcgc tgcagacgac
    13441 cggctgcgcc accaatccct ataccaaacg ctggcagctt ctcatggttc cccagtccta
    13501 tgaagccaat ctaggcgccc aggcgtatca ggacgtcctg agtagcccca aggtcaaaat
    13561 ctccgacaat ccctccgaag tcacaccggt tcagagagtg gcttcacgta tcatcgaagc
    13621 cgcgaagaaa tccaaatatg ccgactcggc aaaagacttc aaatgggagg tcgcggtcat
    13681 taaagacgac ggcaccaaga atgcctttgc cctgccggga ggtaaaatcg cggtgtatac
    13741 cgggatcttc cccgtcgcgg ataatgagtc cgggctggcc gcgatcatgg gccacgaagt
    13801 cgtccacgcg ctcgcccgt
//
LOCUS       DASSCQ010000004         5691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_573180, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000004 DASSCQ010000000
VERSION     DASSCQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573180"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..349)
                     /locus_tag="VFH05_00145"
     tRNA            complement(274..349)
                     /locus_tag="VFH05_00145"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Met,seq:cat)
     gene            complement(431..1885)
                     /locus_tag="VFH05_00150"
     CDS             complement(431..1885)
                     /locus_tag="VFH05_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6676655.1"
                     /translation="MRARILIVDDDPDIVLALENRVTWMGHEPLTAANGKEALRLIQS
                     EQPDLVLLDIQLPVFSGLEILQQMHDSMQKPPDAAGPSLPPIVMLTAFGTIELAVKAM
                     QLGAVDFITKPFTGDHITVVINKALEAIALRRQVGVLKQELEDRYGPIVGSNAKFTAQ
                     LALAEQAAASNVTVLILGETGTGKEVAARAVHGWSPRKHKPFVAVNCAAIPKDLLENE
                     LFGHEKGAFTGAIKREPGKIEIAEGGTVFLDEIGDMSLSLQSHLLRVLQDQTFYRVGG
                     TQSVHTDVRFIAATNKDLKQAIRQGTFREDLFYRLAVISITLPPLRDRIDDIPALAAY
                     FLKRAVKLGLSKQCNMSDAALHTLAQYRWPGNIRELENVLTRAYILSPGKTIEPEHLC
                     LSESVTVGDPIEEQPVQHRTIAGNGYHEMMEAYSRWVLEEALKKNDWNQTRAAEALGL
                     QRTYFTKLLRQKQIPGRQPKPASPSSSSPADMNH"
     gene            complement(1980..4331)
                     /locus_tag="VFH05_00155"
     CDS             complement(1980..4331)
                     /locus_tag="VFH05_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6676656.1"
                     /translation="MTTTKLTRRDASPGNGARTLSSLRTKFVVFFSLILILTCSTLSW
                     YFIEVRRVSMTENLNQLGTILLTSVVNNGQFRYGGLIAEDRATLREFVESLMAVDDVV
                     YVVIRGADHIILAQQNKVVRESSGSITFSQERRYYPDEGLAEEMYRTPPTTPRITRLV
                     LSGDKILVPDGGTSDSMWVLSALTERLYDFALPVLRKPATDPSLSPLLLEMEERIQSK
                     RSAAPYLVQGVVQIGITDAGVKRALLDMIRNVLILTTLLIGAGILGAHFLTLRVTTPL
                     RRLAAVARQLAEGESPAPIPASTYDEVGQLTQTFNSMSQALQERSAAISSNMETIRKQ
                     ITQLTTVHQTSAAITSTLDLHALMDIVLKLLRSNLGFSRMVLMLRHEDRDVAYVAQVA
                     GLSDEIAEAARHLTIPVKEDGTILADLFIHAQPVLARDIDTVCHRMHPAILELARRVG
                     VQSFVCVPLQSHNQTLGFIAGDRGEQACSEEDLNILLTIASHVATAIDNARTYANLAQ
                     LTQHLEQRIQERTRELSVANDRLQDHDRRRTMFVSVASHELRTPMTAIRSFADNMLDG
                     VAGVLSDRQKTYLERIGHNLNRLTRIINQLLDWSRLDMQKEILRLEPLSINQVAMVVI
                     ESLRTVAAEKQVEIRVEAPERLPMIHADRDKIEQILWNLIGNAVKFTPPTGFVTVRFD
                     ALPDGLVQTCVSDTGCGIPPDHLDRVFHEFSKVPSTMPGSQGAQLGLFITKSFITMHK
                     GEIRVESELGVGSRFFFTVPAVAPGAGGISPEIVSPGSSAKTA"
     gene            complement(4338..5348)
                     /locus_tag="VFH05_00160"
     CDS             complement(4338..5348)
                     /locus_tag="VFH05_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HET6676657.1"
                     /translation="MRALTQARQAPRLAMNILASIGRLVSRTDHIIGRLLVGAACVSV
                     CWAWSSPHAVAAEIAILKSSGISAYDQAITGFKSTAPSGAIYSEYDVQGDLEQGRKLA
                     KKIRASEASLVVAVGLKAALAAKLEIVDIPIVYMMILDPSKHNLTAPNMTGTLLEIPA
                     ERQFKILRAFLPGLHRLGMLSKVGGNQAKVKDTIAQAATQSFQLQEYPVESEKAVPQQ
                     LRSLLAATDALLLVPDSTVLTNESVGFILETSLAKRVPVIGFSPEFTRLGALLSLSVS
                     YGEVGRETGLLAKRILEGDRKLPGKAVPVERLKITVNLKTARFLGIDFPKDVNHLIDE
                     TY"
     gene            complement(5336..>5691)
                     /locus_tag="VFH05_00165"
     CDS             complement(5336..>5691)
                     /locus_tag="VFH05_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6676658.1"
                     /translation="WNWDIETDCMFWSPQVDDFLGMPPVPGHKTHRGLLALVHPDDRE
                     LMAHAAKQVMDPFRTDITFEHRVKRSDGMMVSCIWSGHIVRDHAGKAVHVLGTVRATP
                     APNGGAGPKDVTCAH"
BASE COUNT         1184 a         1611 c         1655 g         1241 t
ORIGIN      
        1 caagcacact tatctctacg gcggatttga aggcaggggt gacgattacc gtttttccgc
       61 ccgatgaagg ttgtttggaa gcaggggcta ttttcgaggc taattcagtc gatggtcctt
      121 tgcggtggta gaggatcaac ctctgccctt actttgtcac catttgattt tcttgccaat
      181 tttctcaggt gctcatggaa ccggaaaaat gagtggagaa gtaatcagcc gatgagcagc
      241 cgattcataa gctacgggat tgtagtaggt atttggtggc ggtgacccgg atcgaacggg
      301 tgacccgcgg cttatgagtc cgctgctctg ccacctgagc tacaccgcca cgtcgaaggt
      361 gctggcttgc gaggaacaca attttacccg aggaaggatc tctctgtcca cgaaggaacg
      421 ccggcttctc tcaatggttc atgtcagctg gtgatgagga cgacggcgat gcaggtttcg
      481 gctgacgccc gggaatctgt ttttgacgaa gcaacttcgt aaagtaggtt cgctgaaggc
      541 ccaacgcttc ggctgccctg gtctgattcc agtcgttctt cttcaacgct tcctcgagca
      601 cccaacgact gtaggcttcc atcatttcat gatacccgtt cccggcaatc gtccggtgtt
      661 gaaccggctg ttcctcgatc gggtcgccga cagtcaccga ttctgaaaga cataagtgct
      721 caggttcaat cgtctttccc ggcgagagaa tataggcgcg ggtcagcaca ttctccaatt
      781 cgcgaatatt gccgggccag cggtattggg ccagtgtgtg caacgcggca tcgctcatgt
      841 tgcactgctt gctgagaccc aatttcacgg ctcgcttgag gaaatacgct gctaaagcgg
      901 gaatatcgtc gatgcggtct cgaagcggcg ggagagtgat ggaaatgacc gcgagccgat
      961 agaagaggtc ttccctgaag gtcccctgtc gaatggcctg cttcagatct ttattcgttg
     1021 cagcgatgaa ccgcacgtcg gtatgaaccg attgcgtgcc gcccactcga taaaacgttt
     1081 gatcctgcag cacgcgcaac agatggcttt gtaaggacag cgacatgtcg ccgatttcat
     1141 ccaaaaagac agtcccgcct tctgcgattt caatttttcc gggctcccgc tttatggctc
     1201 ccgtaaacgc gcccttttca tggccgaaga gctcattttc gagcaggtct ttcggaatgg
     1261 ccgcacagtt tacggcgacg aacggtttgt gcttccgggg actccatcca tggacagcac
     1321 gcgccgcgac ctccttgccg gtgcccgtct ctcccaggat cagcacggtg acgttggaag
     1381 cggcggcttg ttccgccaac gcaagctgag ccgtaaattt cgcattgctg cccacgatgg
     1441 gaccatagcg gtcttccagc tcctgcttga ggacgcccac ttgccggcgc aaggcaatcg
     1501 cctccagggc cttgttgatc acgaccgtga tgtgatcgcc ggtgaacggc ttggtgatga
     1561 aatccaccgc acccaactgc atcgccttga ccgccaactc gatcgtcccg aaggcggtca
     1621 gcatgacgat cggtggcagt gaagggccgg cagcatccgg aggtttttgc atggagtcgt
     1681 gcatttgctg gaggatctca aggccggaga agaccggaag ctgaatgtcc aagagcacaa
     1741 ggtccggctg ttcgctttga ataaggcgca gggcctcctt accgttggcg gccgtcaacg
     1801 gctcgtgccc catccacgtg acgcggttct ccaatgccag gacaatgtcc ggatcgtcgt
     1861 cgacgatcaa tatccgagcg cgcatggttc gttcctttca gcagatgact gaaaagtcct
     1921 ggaggtcggc atctcgtgtg tctccgacaa cgcctagacg agggtttccc tcgacgcgcc
     1981 taggcagttt tcgcgctcga gcctggagag acgatttcgg gcgagatgcc acctgcccct
     2041 ggtgccacag ccggcacggt aaagaaaaag cgagaaccga cccctaattc gctctcgacc
     2101 cggatctcac ccttatgcat cgtgatgaag cttttggtga taaacagacc gagttgggcg
     2161 ccttgcgacc ccggcatcgt agaaggcacc tttgagaact catggaacac cctatccaga
     2221 tgatcgggcg ggatgccaca ccccgtatcc gagacgcagg tttggaccag gccgtccggc
     2281 aacgcgtcga accggaccgt cacgaatccc gttggcggcg tgaacttcac ggcgtttcca
     2341 atgagattcc agaggatttg ctcgattttg tcacgatcag catgaatcat gggcaggcgc
     2401 tcgggtgcct caacgcggat ttccacttgc ttttctgccg ccaccgttcg caagctttca
     2461 atgaccacca tggccacctg gttaatgctt aacggctcga gccgcagaat ttctttctgc
     2521 atgtccaagc gcgaccagtc cagcagttga ttgatgattc tcgtcagccg gttcaggttg
     2581 tgcccgatgc gctcgagata ggtcttctgc cggtccgaga ggactccggc cacgccgtcc
     2641 agcatgttgt cggcaaagct gcggatggcc gtcatcggcg tgcgcaattc atgtgaggcg
     2701 acagagacaa acatggtgcg gcgccggtca tgatcttgca ggcggtcgtt ggccaccgac
     2761 agttcacggg tgcgctcttg aatgcgctgc tccagatgtt gggtcagttg agccagattg
     2821 gcatacgtgc gggcattgtc gatggcggtc gcgacgtggc tggcaatggt gagcaagata
     2881 ttgagatctt cttccgagca tgcctgttcg cctcgatctc ccgcgatgaa ccccaatgtc
     2941 tggttatggc tctgcaacgg cacacagaca aacgactgca cgcccacgcg gcgcgccagc
     3001 tcgaggatag ccggatgcat ccgatggcag acggtgtcga tatcccgcgc taacaccggc
     3061 tgcgcgtgaa tgaagaggtc ggccagaatc gttccatctt ccttgactgg aatggtcaaa
     3121 tgccgggctg cctcagcaat ttcatccgaa agtccagcca cttgcgccac gtaggcgacg
     3181 tctcggtctt cgtgccgaag catcaggacc atccgtgaga aaccgaggtt ggagcgcagc
     3241 agcttgagca cgatatccat cagcgcatgg agatccaggg tgctcgtgat cgcggcgctg
     3301 gtctggtgga cagtcgtgag ttgggtaatc tgcttccgga tggtttccat gttggagctg
     3361 atcgccgcac tgcgttcctg cagggcttgg ctcatactgt taaatgtttg cgtgagctgg
     3421 ccgacttcgt cataggtcga cgccgggatc ggcgcagggg attcaccctc ggccaactgg
     3481 cgggcgaccg cagcgagcct tcttaatgga gtggtcacgc gcagggtcag gaaatgcgcg
     3541 cccaagatac cggcaccaat gagcagcgtc gtcaggatca ggacattacg gatcatgtcg
     3601 aggagcgcgc gcttgacccc ggcatcggta atgccgattt gcacgacgcc ttggaccaga
     3661 taaggggcgg cgctccgttt cgattggatc cgttcctcca tttcaagcag gagcggggag
     3721 agcgagggat cggtcgccgg tttgcgaagg acgggcaagg cgaaatcata gagccgttcg
     3781 gtaagcgccg aaagcaccca catgctatcg gacgtgccac catcgggaac caggatcttg
     3841 tccccggata ggaccagccg cgtgatgcga ggcgtggtag ggggggtgcg atacatttct
     3901 tcagccaagc cttcatcggg ataatagcga cgctcctgcg agaaggtaat gcttccggac
     3961 gattcacgga cgaccttgtt ctgttgagcc agaatgatat ggtcggcccc ccggatcacc
     4021 acatacacca cgtcgtcgac cgccatcagg ctttcaacga actcacgaag cgtggcccgg
     4081 tcttcggcga ttaagcctcc ataccggaac tggccgttgt tcacgacgct ggtcaatagg
     4141 atcgtgccca attggttgag gttctccgtc atcgagaccc gccgcacctc aatgaaatac
     4201 cagctcaatg ttgagcaggt gaggatgagg atcaagctga agaagacgac gaacttcgtg
     4261 cgaaggctgg aaagcgttcg cgcgccattg ccaggcgaag catccctgcg tgtcaacttc
     4321 gtggtcgtca tggtcgatca ataggtttca tcgatcaagt gattcacgtc tttgggaaaa
     4381 tcgatgccaa gaaaccgcgc cgtcttgaga ttgacggtga tcttcagccg ttcgacagga
     4441 acggctttgc ccggaagttt ccggtcaccc tctaggatac gctttgccaa caagcctgtc
     4501 tcccggccca cttcgccgta ggacactgaa agactcagca gcgctcccaa cctggtgaat
     4561 tccggcgaaa atccaatgac cggcacccgc ttcgctaaag atgtttccag gatgaatccg
     4621 acggattcat tggtcagcac tgtcgaatct ggcaccaata acagggcgtc cgtagcagcc
     4681 agaagtgacc ggagctgttg ggggacggct ttctcgcttt cgacaggata ctcttgcagt
     4741 tggaatgatt gggtggcggc ttgcgcgatg gtgtccttga ccttggcctg gttgccaccg
     4801 accttggaca acataccaag acgatgcagg cccggaagga atgcccgcaa gatcttgaac
     4861 tggcgctccg ccggtatttc cagcagcgtt ccggtcatat tgggagcggt cagattatgc
     4921 ttggacggat ctaatatcat catatacacg atgggaatgt cgacaatctc aagcttggcc
     4981 gccagcgcgg ctttcaagcc cacagcgacc accagcgacg cttcagatgc ccgaattttt
     5041 ttggcgagct tgcgtccctg ttcgagatcg ccttgcacgt cgtactcaga ataaatggct
     5101 ccggacggcg ccgtcgattt gaatccggtg atcgcctgat cgtaggcact aatgccggag
     5161 gatttcagga tggcaatttc ggcggccact gcgtgagggc tgctccaagc ccagcagacg
     5221 gacacgcacg ccgctcccac cagcagacga ccaatgatgt ggtccgtcct gctgacgagg
     5281 cgccctatgg aggccaagat attcatcgca agccgggggg cctgtcgagc ctgagtcaat
     5341 gcgcgcatgt gacgtccttg gggccagcgc cgccattcgg ggcgggtgtc gctcggacgg
     5401 tgcccaacac atgcaccgcc ttgccggcgt gatcccgcac aatgtgtccg ctccagatgc
     5461 aggacaccat catgccgtcg gatctcttca cacgatgctc gaaagtaata tccgttcgga
     5521 agggatccat gacctgcttg gccgcatgcg ccatgagctc gcgatcatcc ggatgcacca
     5581 aggcaagcag tccgcggtgc gtcttgtgtc ccgggacagg cggcatgccc aggaagtcgt
     5641 cgacctgggg tgaccagaac atgcaatcgg tttcaatgtc ccaattccag a
//
LOCUS       DASSCQ010000005         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_955287, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000005 DASSCQ010000000
VERSION     DASSCQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955287"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1091
                     /gene="rfbB"
                     /locus_tag="VFH05_00170"
     CDS             51..1091
                     /gene="rfbB"
                     /locus_tag="VFH05_00170"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET6676659.1"
                     /translation="MNILVTGGAGFIGSHLVRRLVVQSGHYVVNLDALKYSGNRANTL
                     DLEGHARYRFVHGDIADQPLVHSLLQDHEIEAVINAAAETHVDRSIVDPGSFAKTDVV
                     GTGILLEEARRARIKRFLQVSTDEVYGSVEAGMSTEEDVLAPRSPYSASKAGGDLLTL
                     SYWTTYRFPVMVSRGSNTYGPNQYPEKFIPLFLTNALDDQPLPLYGDGRQRRDWLAVE
                     DHCAAIEQIVLNGEPGEVYNVGGGNERENIAVAERLLALLGKPKSLIRLVEDRPGHDR
                     RYAVDCRKINRMGWQPTVSFDAGLKATVDWYRQNESWWRPIKSGEFRAYYEAMYAQRL
                     KEGLSASSSCGS"
     gene            1079..1927
                     /gene="rfbD"
                     /locus_tag="VFH05_00175"
     CDS             1079..1927
                     /gene="rfbD"
                     /locus_tag="VFH05_00175"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET6676660.1"
                     /translation="MRILITGANGQLGSALQRTLTGEDLVLKDLPDFDLTTPTCEDQI
                     RDARPAVIVHVAAYTNVDGAEREPERAAAVNVQGTTYVARAAAALHARLLYLSTDYVF
                     DGTKMSPYCEEDSPRPLNQYGRSKYEGERAVLSLCPNSVVVRTAWLYGHDGQNFVKTI
                     MKLAQERPVLEVVSDQRGCPTYADDLARALKSLVSSGAQGIYHVTNGGDASWHEFAQA
                     IVRHVGLSTAVHPISTVQSGRPAKRPAYSVLSGDRFARDFFPLPTWQDALYRFMKGAP
                     QLVSPL"
     gene            2025..2552
                     /locus_tag="VFH05_00180"
     CDS             2025..2552
                     /locus_tag="VFH05_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET6676661.1"
                     /translation="MAARKRQRPRKSLGATLKRIRLFATDVDGVLTDAGMYYSESGEE
                     LKKFNTRDGMGIKLLQRAGLITAIVTQERTKLVAWRGEKLMMPEVHQGVMDKLSLVRE
                     MAARHGLSMDEVAYIGDDINDLATLKAVGFSATPADGMPAVVEAVDYVCVKKGGEGAV
                     REIIEMILKAQQKAP"
     gene            2569..4020
                     /locus_tag="VFH05_00185"
     CDS             2569..4020
                     /locus_tag="VFH05_00185"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HET6676662.1"
                     /translation="MGDKPTLYPVIMAGGSGTRFWPLSRQLFPKQLLKIIGDETLIQQ
                     TMRRVVKGAPPERVLISTNPAQAESIRVQLSEWKDALKDNFVLEPEGRNTAPAIALVA
                     LELVRRDPEAIMLVVPADHIVKGQRDFDAAVSVAASLALEGLLVTFGIKPIRAETGYG
                     YIKPNTKTVLARQGKLKGYRVDRFVEKPNAMKAAQYLKAGDYYWNSGMFVWRAATILE
                     EIRRHQPALADGIDRIGRLINQGAAKAAIDEEYRLLPSISIDSGVMERSNKAAVVPVS
                     FRWSDVGSWGSLDEVARKDRAGNVVGGRVVDIGSQRSVVYGDRRVVATIGLIDMVVVD
                     TPDATLVCPKSRAQDVKKIVDILKQQGAPEHLEHLTVHRPWGSYTVLEEGPGYKVKRV
                     TVHAGGRLSLQLHHKRSEHWVVIAGTARVTRGEEVFELHVGQSTAIPVETRHRLENFG
                     QDTLHIIEVQNGPYLGEDDIVRFKDDYGRTAAS"
     gene            4035..>5298
                     /locus_tag="VFH05_00190"
     CDS             4035..>5298
                     /locus_tag="VFH05_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET6676663.1"
                     /translation="MGLFREYDLRGIVGDDLTEAIAEQVGRAYCTYVKARGVQTISVG
                     RDGRLSSPALFKSLVSGLLAGGVNVIDIGICPSPLLYFSLFQLPVQGGIMITGSHNAA
                     EYNGFKVCVGKEAIHGETIMELRRILETGSFTSGQGRLSEHTIIPDYLEYLRTSFKGV
                     NAGRLHVVIDCGNGAAALVAKEALEILGCRVTGLYCDLDGRFPNHHPDPTVLENLDDL
                     VKAVKQHKADVGIGYDGDADRIGAIDEQGHVLWGDRLMVVYSRDILRTIPGSTIISEV
                     KASQCLYDDIAKQGGRAIMWKTGHSLIKAKMKSEKAVLAGEMSGHMFFADRYFGYDDA
                     IYASCRLVEILAKTGQSLSSLVADLPATSVTPEIRVDTPDSIKFELVKRVQDRLAGYA
                     KSSRPIGPNNDVVRDVVTIDGVRAIFEDG"
BASE COUNT         1164 a         1394 c         1645 g         1095 t
ORIGIN      
        1 gtgaatgaac gaatgatcaa tcgggcggtc agacacatgg gaatgcagtc atgaacatac
       61 tggtcacggg cggcgccggc tttatcggct cacatctggt tcgtcgtttg gttgttcaga
      121 gcggacatta tgtcgtgaat ctcgacgcgc tcaaatattc cggcaaccgt gcgaacacgc
      181 tggatttgga gggtcatgcg cgttatcgat tcgtccatgg agatatcgct gatcagccgc
      241 tggtccatag cctcttgcag gatcacgaga tcgaagccgt gatcaatgcg gcggcggaga
      301 ctcatgtgga ccgatcgatt gtggaccccg gcagcttcgc caagacggat gtggtgggga
      361 ccggcattct gctcgaagaa gcgcgacgcg cccgtatcaa gcgcttcctg caggtgagca
      421 ccgatgaagt ttatggctct gtggaagccg ggatgtccac ggaagaagat gtgctcgcgc
      481 cgcgcagtcc ttactcggca agtaaagcgg ggggcgacct cctgacactg agctattgga
      541 ccacctatcg gttccctgtc atggtctcgc gcggaagcaa cacctacggt ccgaatcagt
      601 atccggagaa attcattccg ctctttctga cgaatgcctt ggatgaccag ccattgccgt
      661 tgtatggcga tggtcgccag cgacgcgact ggctggccgt cgaagaccac tgtgccgcaa
      721 tcgagcagat tgtgttgaac ggagaacctg gagaggtcta caacgtcggc ggagggaacg
      781 aacgggaaaa tattgcggtg gcggaacgac tcctggcctt gcttggcaaa cctaagagct
      841 tgatccggct tgtggaagac cggccagggc atgatcgacg ctatgcggtc gattgccgca
      901 agatcaatcg gatgggatgg cagcccaccg tctcgttcga cgcggggctc aaggccacag
      961 tcgactggta tcgacaaaac gagtcctggt ggcgtcccat taagtccgga gaatttcgtg
     1021 cctattacga agcgatgtat gcccagcgtc tgaaggaggg gctctccgcc tcatcgtcat
     1081 gcggatcctg attaccggcg caaacggaca gctgggaagc gcattacagc ggacgttgac
     1141 aggagaggat ctcgttctca aggacctgcc ggattttgat ctgacgaccc cgacctgtga
     1201 agatcagata cgagacgcac gtcccgcggt aatcgtgcat gtcgcggcct atacgaatgt
     1261 ggatggcgcc gagcgtgaac cggaacgtgc cgccgccgtg aacgtgcagg gcactaccta
     1321 tgtggcccgt gcagcggccg cactgcatgc acggctcctg tatctttcga ccgattacgt
     1381 cttcgacggc acgaagatga gcccctactg tgaagaggac agccctcggc cgttgaacca
     1441 gtatgggcgg tcaaagtatg aaggtgaacg cgctgtgttg tcgctctgcc cgaacagcgt
     1501 ggtcgtgcgg accgcttggc tgtatggcca tgacggccag aatttcgtga agacgattat
     1561 gaagctggcg caggagcggc cagtgcttga agtggtgagc gaccagcggg gctgcccgac
     1621 gtatgcggat gatttggccc gtgcgctcaa gtcgctcgtg tcgagcggcg ctcaggggat
     1681 ctatcatgtg accaatggcg gcgatgctag ctggcatgag tttgctcaag ccatcgtccg
     1741 tcatgtgggc ctgtcgaccg ccgttcatcc tattagtacc gtccagtccg gacggcccgc
     1801 caaacgaccc gcctactcag tgttaagcgg cgaccgcttt gcccgcgatt ttttcccact
     1861 tcccacatgg caagatgccc tctataggtt tatgaagggt gcaccacagc ttgtctcgcc
     1921 gctctgaggt cttcacacac catcactttt tgttttgaac tccatagctg agccagtaag
     1981 atgggcctgg ccagtgaggt gttaatccag cgagaccgat tcttatggcc gctcgaaaac
     2041 gacaacgacc aagaaaatcc ttgggagcaa ctctcaaacg aattcggctc ttcgcgaccg
     2101 acgtcgatgg ggtgctgacc gatgccggca tgtactattc agaatctgga gaagagttga
     2161 aaaaattcaa cactcgagac ggcatgggga tcaagctgtt gcagcgggcc ggactcatca
     2221 ccgccatcgt gacgcaagag cggacaaaat tggtcgcctg gcgaggggaa aagctgatga
     2281 tgcccgaggt gcatcaaggc gtgatggata agctgtcgct cgtgcgtgag atggccgcac
     2341 gtcatggcct ttcgatggac gaggtcgcct atatcgggga tgacatcaat gacctggcca
     2401 cgttgaaggc cgttgggttt tcagccacgc cggccgacgg gatgccggcg gtagttgaag
     2461 cagtggatta tgtctgcgtc aaaaaaggtg gcgaaggcgc ggtgcgcgaa atcatcgaga
     2521 tgattctcaa ggctcagcag aaggccccct aaacaatcga ggcgagcgat gggcgacaag
     2581 cctactcttt acccggtgat catggccggc ggcagcggga ccagattctg gccgctgagc
     2641 cgccaattat ttcccaagca gttgctgaag atcatcggcg acgaaacgct gattcaacaa
     2701 acgatgcggc gggtcgtgaa gggagcgccg cctgaacggg tcctgatctc gacgaaccca
     2761 gcccaagccg aatcgatccg agtgcagctc agtgaatgga aggatgcgct caaagacaat
     2821 ttcgtgctcg agccggaagg ccgcaacact gcgccggcga ttgctctggt tgcgctggaa
     2881 ctggtccgtc gagaccccga ggcgattatg ctggtggtgc cggccgacca cattgtgaag
     2941 ggacagaggg attttgatgc agccgtctcg gtggcggcgt ctcttgcact cgagggtctg
     3001 ctggtcactt tcgggatcaa gccgattcga gcagaaaccg gatacgggta tatcaagccg
     3061 aatacgaaga cagttctggc gcgccaggga aaactgaagg ggtatcgggt cgaccggttt
     3121 gtcgaaaaac ccaatgccat gaaagcggcg cagtacctca aagcaggtga ttactattgg
     3181 aatagcggca tgttcgtgtg gcgggccgcc acgattttgg aagaaatccg ccggcatcag
     3241 cctgccctgg ccgatggaat cgaccggatc ggccggttga tcaaccaggg tgcggcaaaa
     3301 gccgccatcg atgaagaata tcgcctgtta ccgtccatct ccatcgatag cggagtgatg
     3361 gagcgatcca acaaggctgc ggtcgtgccg gtgtcgttca ggtggtcgga cgtcgggagc
     3421 tggggcagcc ttgacgaagt cgccaggaaa gacagggccg gtaacgtagt gggtgggcgc
     3481 gtcgtggaca ttggtagcca acgttccgtg gtgtatggag accgtcgcgt tgtggccacg
     3541 atcgggttaa tcgacatggt tgtcgtggat acccccgatg ccacacttgt ctgtcccaag
     3601 tcccgcgctc aagacgtcaa aaagatcgtc gatattctca agcagcaagg agccccggag
     3661 cacctcgagc acctgacggt ccatcggcct tggggttcct atacggttct cgaagagggc
     3721 cccggataca aggtcaagcg agtcacggtg catgcgggtg gtcggctgtc attgcagctg
     3781 catcataagc ggagcgagca ttgggtcgtg attgcgggga cggcccgtgt gacgagaggt
     3841 gaggaggtgt tcgaactgca cgtggggcaa agcacggcca ttccagtaga aacgagacac
     3901 cgcctcgaaa atttcggtca ggacaccctc cacattattg aagttcagaa cggtccctac
     3961 cttggggaag atgacatcgt gcgtttcaaa gatgattatg ggcggacggc tgccagttaa
     4021 cacagggagc gttgatggga ctgtttcgag aatacgatct tcgaggcatt gtgggggatg
     4081 acctgacaga ggcgattgcg gaacaggtcg gtcgcgccta ctgcacgtat gtcaaggcac
     4141 gtggtgtcca gacgatttcc gtcggtcgcg atggcaggct gagctctccc gccttgttca
     4201 aatccctggt cagcggcttg ctagccggtg gcgtgaacgt catcgatatc ggcatttgtc
     4261 cgtctccatt gctctatttt tcactgttcc agcttccggt tcaaggcggc atcatgatca
     4321 cgggcagcca caatgccgcc gagtacaacg gatttaaggt ctgtgtgggc aaggaagcca
     4381 tccatggcga aaccatcatg gagcttcgac ggatcctgga aacggggagt ttcacgtccg
     4441 gtcaagggcg gctcagcgag catacgatca tacccgacta tctggagtac ctccgaacaa
     4501 gcttcaaggg cgtcaatgcc gggcggctgc atgtggtcat cgactgcggg aacggtgcgg
     4561 cagcattggt cgccaaagaa gcgctcgaaa ttttgggctg ccgcgtgacc ggcctctact
     4621 gtgatctgga cggccggttt ccgaaccatc atcctgatcc gacggtgctg gaaaatctgg
     4681 acgacttggt caaagcagtc aaacagcaca aggcggatgt cgggatcggc tacgacggag
     4741 acgctgatcg gatcggcgcc attgacgagc aaggccacgt cttgtggggt gatcgtctga
     4801 tggtggtcta ttcccgggat atcttgagga cgataccggg cagcacaatt atttccgagg
     4861 tcaaggcgtc gcaatgcctc tatgatgaca tcgcgaagca aggcggccgc gcgatcatgt
     4921 ggaagaccgg tcattccctc attaaagcga aaatgaagag cgagaaggcc gtgctggcgg
     4981 gagaaatgtc cggccatatg ttttttgccg atcgatactt tggctatgat gatgcgattt
     5041 atgcctcctg ccgccttgtc gagatattgg cgaaaaccgg ccaatcgctt tccagcctag
     5101 ttgcagatct gcctgccaca tccgtcacac cggaaattcg agtggatact cctgattcga
     5161 tcaaattcga actggtcaaa agggtgcagg accggctagc cggctatgcg aagtccagtc
     5221 ggcctatcgg cccaaacaac gacgtcgtcc gcgacgtggt gacgatcgac ggggtgcggg
     5281 ccatctttga agacggat
//
LOCUS       DASSCQ010000006        40088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_382255, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000006 DASSCQ010000000
VERSION     DASSCQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 40088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382255"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..437)
                     /locus_tag="VFH05_00195"
     CDS             complement(78..437)
                     /locus_tag="VFH05_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676664.1"
                     /translation="MGPTKAIIKDQALYEAATGKCIKDGFASRRDIEDYVNHHYLALP
                     VVDNAGNAWLLDGKPIYCFRGSQYETLDDQRVHLARCPDCGGMGIRSDEFTVESDCIR
                     CISCGHEFDARLEMMET"
     gene            complement(532..1305)
                     /locus_tag="VFH05_00200"
     CDS             complement(532..1305)
                     /locus_tag="VFH05_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6676665.1"
                     /translation="MASTRIASKKPRPSLDRSIERMRKHLAELAVYVKKGKASRTLEE
                     FDSDSERLIAELLGETSALLDAYQYAQLGEAAGLVNMTEEAPESIGLDSERQSLLQRT
                     RVLESCVSELEARRADEPKKLQSGKHMLTGPQVAEHMSSDLRSVPQTATLREAGRLMQ
                     KWKTGSLLVTDSQTYVGFITDSALARDVVANGLDANTTSVQSCVRKPLVAIHGDRPLI
                     EAVRLMKEQATRHLAVAQDGEIIGVISVSNILRYYSGVV"
     gene            complement(1395..1859)
                     /locus_tag="VFH05_00205"
     CDS             complement(1395..1859)
                     /locus_tag="VFH05_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676666.1"
                     /translation="MRVLVTSLWILCLWSGCGIIMVGEPVQKLDISSQDDVEENLKAI
                     RAILADQETRKAPSVAKPSHPSSRAAPASESGPVSEPLLHPTPAPSSSSIVAGHLDAP
                     ASLPWSPSPAQRPEVPDRPVPAYTVPAPVAPDYSGSIRCAPDGLGGQRCAGR"
     gene            complement(2127..3872)
                     /locus_tag="VFH05_00210"
     CDS             complement(2127..3872)
                     /locus_tag="VFH05_00210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6676667.1"
                     /translation="MKHDHTHAPEATAVHGFSPGFAALGLEARLLATLEALGYEEPTP
                     IQREAIPPLLAGKDVLGQAATGTGKTAAFTLPLLQRIAHSPKTKPSAIILVPTRELAI
                     QVGEAVGRYGKELRTSVLPLYGGQAIGPQMHALKRGVDVIVATPGRALDHIRRHTLKL
                     EAVQIVVLDEADEMLDMGFAEDLDAILEHTPAAKQTALFSATMPPRIQSIAHRHLQQP
                     VEITIAKEAVKAGSAPRVQQAAYIVTRPYRGATLARIIETAAPKSALVFCRTRVEVDE
                     VTLMLSARGHRVEAIHGGLSQVQRDRVMQAFRTGQTELLVATDVAARGLDIPQVSHVI
                     NYDLPSSAEVYVHRIGRTGRAGREGTAITILDPREQRLLRSIEQHTKGKVVVTPVPSV
                     ADVVAKRLERTRSAVQEAIRAGDLDGFRGVVKAMAEATDLATIGAAAMKLLFRAEGGE
                     RPEQEIPALPMRQAEWHPSAQREGLHRDGASRNRHGRQAMPPAKNRTLRGREAGMTRL
                     YVGAGRDAGIRAGDLVGAIANEAHINSNLIGAIQIEERFSLVDVPESLAKGIVEVLGR
                     SRIKGRKVGVRVFRE"
     gene            complement(4045..4725)
                     /locus_tag="VFH05_00215"
     CDS             complement(4045..4725)
                     /locus_tag="VFH05_00215"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HET6676668.1"
                     /translation="MARSLGDPIQRLMGLMFKAHPWHGVSIGEKAPDVVTAYIEIVPT
                     DTVKYEVDKDSGFLKVDRPQRFSNYCPVYYGLVPQTFCGNKVAAIFSKRAKRKGMVGD
                     GDPLDICVLTEKTIPHSDILLTAIPIGGFSLADGGEADDKIIAVMKDDAAYGSFTDIG
                     DCPAPLMERLQHYFLTYKQAPGSLQHKVEITGIYGRDEALKVLRASHADYREKFPELE
                     SLWPKQLG"
     gene            complement(4725..5171)
                     /locus_tag="VFH05_00220"
     CDS             complement(4725..5171)
                     /locus_tag="VFH05_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676669.1"
                     /translation="MKPVIQLERTGCGIASVAALAGVSYQAARQAAGRLGISAEDHRL
                     WSETTHVRRLLKRYRFYASSRERPFRSWPALPKRSLLAIKWRRIKGRACWHWVVCVKD
                     DSMYVLDSSPSLTRHVRTDFRRMKPKWFIAVTRLPSKRSRTLSRRN"
     gene            complement(5171..5641)
                     /locus_tag="VFH05_00225"
     CDS             complement(5171..5641)
                     /locus_tag="VFH05_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6676670.1"
                     /translation="MTAHIDLARPEDAEVIAAMVGELLQEIMSAVSEKAFNFHHRDTE
                     ARARAWLIDGSYLVLLARDGEAAVGFLALYQSYALYTEGAYGTIPEFYVRPSHRSRGI
                     GTALIAESKKVGQARGWRRLEVTTPPLPQFDRTLAFYQRHGFAISGGRKLKVLL"
     gene            complement(5830..6027)
                     /locus_tag="VFH05_00230"
     CDS             complement(5830..6027)
                     /locus_tag="VFH05_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676671.1"
                     /translation="MTVYQWTRTKPLGAGWYWFRGEAHEADPFIVQVDEAGQFQWPDG
                     GYQEVRLAKGEWAGPIEEPAE"
     gene            6135..8909
                     /gene="hrpB"
                     /locus_tag="VFH05_00235"
     CDS             6135..8909
                     /gene="hrpB"
                     /locus_tag="VFH05_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HET6676672.1"
                     /translation="MNRLPIEDVLPVLRDTLTQGSNAVLSAPPGAGKTTRVPLALLDA
                     PWLTGKTILMLEPRRLAARAAAHRMASLLGESVGSTVGYRMRLDSKIGPVTRIEVVTE
                     GILTRLLLDQPELDRYGAILFDEFHERSLQADVGLALCLETQRLVRPDLRLLVMSATL
                     DCGPVSELLGDAPIIISEGRMFAVETRYLTRPHTGPLEAAVTQAIRRSLTQDQGSVLV
                     FLPGMAEIRRVERQLLDSNPGPGVVIAPLHGDLPQSAQDKAIVPAESGTRKVVLATSI
                     AETSLTIDGVRVVIDAGQLRVPRFDPRSGLTRLETVRVTQDSADQRKGRAGRLESGIC
                     YRLWTEREHLSLAPRKTPDILEADLAPMVLDLAQWGETDPSRLRWLTLPPSGAVAQAK
                     DLLIRLGALDISGRLTPHGRQMAGLPLHPRLSHMLLKSLTLGCSNLACELAALLSERD
                     FLRGPSATRQTDLRLRIDVLHDIKKHVASGTIDRGTIERITRTARQWKTQLARVHRLP
                     GGGSSGTDAVGLLLALAYPDRIAQRQPGHEARYVLANGRGALFAYPDPLASEPFLVIA
                     ALDAGTPWARIDLAAPLSAKEIEQLYAGQIEETEAISWDDRTRSVRASRQRRFGALIL
                     SEEALSKADPALITKALLDGLRRAGLATLAWTPELQEWRRRIQFLRGLGNRDVSASRG
                     REGEIGNRDVSTSRGREGEIDNRDVSASRGREGEIDNRDVSASRGREGKIDNRDVPAS
                     RGRESETDSDITSTREGKGANLTWPDLSDERLLNTLEDWLGPYLHGITTLERVQRMDL
                     TQPLHALLTWNQQRDLDRLAPSHLTVPSGSTIRLDYETPDLPVLAVRLQEMFGCRETP
                     RIANNTVPVLLHLLSPAKRPVQVTRDLASFWVNGYPAVRKELRGRYPKHHWPDDPLIA
                     VPTAKTKRRT"
     gene            8965..9609
                     /locus_tag="VFH05_00240"
     CDS             8965..9609
                     /locus_tag="VFH05_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676673.1"
                     /translation="MVQCLPFLLSLAVIVPIAIGPTIALADVPALAPPPPSASFDDVD
                     DITVQAEVTGQALAFLTRLQRHWGESADRVRTLIAGTIERDQEGTLIYLRNFRDHGVL
                     EGYQFRKGSLTCGRYVMVQRPLNGLNEFIGYYASLKGALHSKYGDPEFDRVVWDNDLY
                     RPLPDYWGVAVMIGHLRFHASWNTVDGTIALELTGNRHSRLSLESCTTREEAQT"
     gene            complement(9655..9933)
                     /locus_tag="VFH05_00245"
     CDS             complement(9655..9933)
                     /locus_tag="VFH05_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6676674.1"
                     /translation="MVKVLVFGLTLRDAVGTHEVELDVPQVTTVKKLIDAYPTELGAV
                     APFMQQREVLVAVNKKVGSEDSPVKDGDVVKLTHQSKTSYDGVRDIPT"
     gene            complement(10010..10249)
                     /locus_tag="VFH05_00250"
     CDS             complement(10010..10249)
                     /locus_tag="VFH05_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676675.1"
                     /translation="MRLRRADWLFVVFAVTVVAGVSFLPTPKDRNPMVPRTAEHQAAK
                     SEQQCVGCHKSDGVKPLPARHPKRPDCLRCHARQA"
     gene            complement(10246..13065)
                     /locus_tag="VFH05_00255"
     CDS             complement(10246..13065)
                     /locus_tag="VFH05_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HET6676676.1"
                     /translation="MPDTSRAIGADLRRLLKPDQIKDDAPTKTAYAVDASIYRMEPQA
                     IALVESEDDIQRVVSYAMNRSVPLTARAAGTNLTGSAIGSGIILDVSRLHRILEVNRE
                     ERWARVQPGIILAELNKQLAREGLMFGPDPSSGDMCKLGGMLANNSAGPHTLRYGAVK
                     DNVRSLRVCLASGSWLDARNFDLDDPALAGVLSSHAAIRETLSLIRAHADLLARKRPA
                     VSKNSCGYNLFGLADGLPQGRLHLPHLFVGSEGTLGIFSEATINLVPKPRATLTALIH
                     FKRLEDVGDAVPRLLALIPSALEVMDANTLDLIGRSTHGIPAAAAATLLAELEAESGA
                     VDLREAAERMATICGDYPLASEIVVAYDPERREQLWKARKALYPTLYRFDPRKKPINF
                     VDDVVVRAERISELIRYLEEFFGSQRVPVAIFGHIGNGNAHIVPLLDVNDKRDFDKMV
                     QAYHEIHQTVLNRFEGSICGEHGDGRVRAEYVQAMFGPELYDLFVKVKRSFDPSGMLN
                     PGVKISDRPFTDHIDYTRLSKSCATCAKCNAVCPVYDVFRSEDMSSRGWFEIVTDAQY
                     SYLNSKRVVEACLNCKSCRTACPAGVDVSQLILDKRAEHPNKLAGAIFRFQAKTSQFA
                     AFLKWVARFQPLWDRPLIRRLLDRVSRPILSRLAESARFSPAIAMPKFASRHLRDRYP
                     QLALDPEQRRRSAVAYFHGCAANYFDDGVGDAVIAVLRKHGVEPDLPAQRCSGTPIET
                     YGHRELAKDGARINLRSFTGYETIVTGCASCTLMLKDYPKLFAGEPEQSAAEAMARRV
                     RHISEFVAQSPVRPPMATVGAARTVTYHSSCHLRAAGVTKEPRAILQNLPGVQYVEMP
                     DADRCAGGAGTYLVKDFETSQRIVSRKRQAVAQSGAGVVATSCPACMIQLKTGLPADV
                     AVKHVAQVLRESYEAGERQTGEPHP"
     gene            13158..14120
                     /locus_tag="VFH05_00260"
     CDS             13158..14120
                     /locus_tag="VFH05_00260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET6676677.1"
                     /translation="MASAKPLLYVSRLLPDPVMAAVRAQYDLLHEPSNGPAAPAALRQ
                     GLSQAVAAIVTLTDRIDADTLASARELKVLANYAVGYNNIDLNAAQRRGVAVTNTPDV
                     LTDATADLTWALLLAAARRVVEGDRLVRSGQWTGWTPTQLLGAAVAGSIVGIVGMGRI
                     GQAVARRAAGFRMPVIYHSHRAVDGPPGAHWASRDLPDLLRESDIVTIHVPLTSETRH
                     LIGAPEFALMRRTAILINTARGPIVDESALVTALQEGTIAGAGLDVFEDEPKIHPHLA
                     SLPQVVLLPHLGSATLATRVEMGMICLKNIEAILAGRPAPNRVA"
     gene            complement(14164..16332)
                     /locus_tag="VFH05_00265"
     CDS             complement(14164..16332)
                     /locus_tag="VFH05_00265"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676678.1"
                     /translation="MGIDRSKVLHSAQLFASKGQFDAAISEWKKLSAESPGDGSIHNS
                     IGELHLKRNSTADAVASFIQAANAFRAEGATLKAIATFKKVLKLDPSRYDMYRFLGDL
                     NAERGLLSSAVQDYLTLGKHYLKERKTKEALDIYKKIVSQDPSNLDAQQRVAELCVQE
                     NMQDEATRVYLQLGRERSGQQRYAEAKEAYESVLRIDPSNSEAQQFIEHYKKTGGTAP
                     PRTTKPGSTPAAHTGEPTDLLAEASRRIEEKQYAGAEAILNQMLTKEPGNPQVCQLLA
                     RLHLQQGQLQVALGEYRFLAGAALRAQDYPQAETLIREFLAVEPNSVPLLELQGELYE
                     EKGEIESAIQHYGKAIEVLLKTPEPGMPTLHEEIFEKVQTLAPDSPTAKRLAALMRGE
                     ATVEPAAAAAPATANGGFSIEGATPDDAWNMTSAIKPTQDRHLAGQAVPVGLPPSAPS
                     SPEVFSVENATPDDLPSLSAEAPEPVASGQAALSISEQFQAYTESGQWAQAEQWLGTV
                     VSAQPADPHPRELLGQVLERKGDGAGAALQYARALELMSAGQDSVVNDPIRHLYDKVK
                     DLAPASPIVAKLSATMAPAAPADEVDAETHYTLGVAYKNMGLIEEAKEEFAFAMKGPE
                     VFLDACLMTAVCLKEQERYDSASAQLERLLTDPRCQGAKAQAIRYELGLLYEQQQQWQ
                     LAATMFEAIPAFHDVPQRLAAIRTVPKPASASAQAFRYAT"
     gene            complement(16487..16705)
                     /locus_tag="VFH05_00270"
     CDS             complement(16487..16705)
                     /locus_tag="VFH05_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET6676679.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDSGEDVFVHYSALQGEGFKSLKE
                     GENVEFDIVQGAKGPQAANVMKMSIAAS"
     gene            17013..18101
                     /gene="lptF"
                     /locus_tag="VFH05_00275"
     CDS             17013..18101
                     /gene="lptF"
                     /locus_tag="VFH05_00275"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HET6676680.1"
                     /translation="MTLRTLLDRYIFTELLYPFSVSLGMLCFIMLTRELLRLVELLVT
                     KGVGLWAVLNVFAHLLPSFLVLTLPIAGIIASITAFGRLSLDKELVAMRAAGLSLLRL
                     ARPVMLFALLVCGMTLWLAQWGQPWSSINLKKVALSLLRDQLVLALERGSFTEPIPGM
                     TIYVSDKGEQPSGGIFISDGRIASEPRVIVAQDYRVLIDSASDQVALRLQDGVIHTKP
                     DEIDEYQLASFSSYDLKLSLNQSGYASTEERPSYDQLVARLNASQWRDPWALRRLTEY
                     YKDMAFPTASLILCMLGLPVGIVSKRSGRIGGFAVGVLVIIAYYVLNVACEFLVTATA
                     IPPFAGAWLPNSVFLVITLVSFYRMSKQ"
     gene            18108..19199
                     /gene="lptG"
                     /locus_tag="VFH05_00280"
     CDS             18108..19199
                     /gene="lptG"
                     /locus_tag="VFH05_00280"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HET6676681.1"
                     /translation="MPILFRYLLREYAKIFLMCFAGLMTIYLVIDFFEKVRRFLSFDA
                     TWLDVLSYFALKIPGVSYQIVPLAVLMATLLTLGLLSRNNEITAIRSCGISLPWIASP
                     FLTFAVLMSLVLLLFSSTVIPLATNKAEEIRVIRIEKKPPSAVLTLSQPWTRVDEDSL
                     LHVSSLAPGGATLGGIQLFHFDPAFRLIDMTEAQEAHYADHTWTLHRGFQRRFNRDGS
                     VTLMEFDRKPIQLAMIPSDFSSGLAGDSEMMTFQDIRESRGRLYQNGSQFARLLTDYY
                     GRIAFPLVTIIMVLVGIALSLRRSGTRGGSMAIGIGQALLVGFSYWAAHSIAIAFGRG
                     GALPPVIAGWLTNVLFTSYGLYLMLKLKY"
     gene            19306..20562
                     /gene="fabF"
                     /locus_tag="VFH05_00285"
     CDS             19306..20562
                     /gene="fabF"
                     /locus_tag="VFH05_00285"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET6676682.1"
                     /translation="MPGQVVVTGLGIVSPIGIGVSEFWKAALAGHSGVSMITSFEPFS
                     MEGYRSKVAGQVRGFSPERYLDSVQAERVDRYAQFALVAAKEALADSGLQMAREDPRR
                     VGVIVGAGMGGMVMGEREITQLYQHQRPHRVHPNFIPVITLNSASGIVAMAHGAKGPN
                     YTVSTACSSSAHALGQAMQCIKEGRADVVITVGADASITPLVFAGFCSLRALSTKYND
                     QPERASRPFDRLRDGFVMGEGAGALVLESSVHAKKRRARIYAEVAGYAATSEAYHMVI
                     PREDGEEIATTMRLAMADAGLTPANIDYINAHATSTTIGDAVETKALRQVFRTRADKL
                     AINATKSLVGHTLGAAGAIGGIATVLAIHTGQIHPTANYEEPDPACALRGLSSSVQER
                     KVKAALLNAFGFGSNNAAVVIKRYAA"
     gene            20593..20889
                     /locus_tag="VFH05_00290"
     CDS             20593..20889
                     /locus_tag="VFH05_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6676683.1"
                     /translation="MSDRAVTEKIIQALADYLRRNPESIDPKHHLRDDLGLDSMAVIE
                     LLYKIEEAFDIQIPDEDLVRLSTVGAVCQYVEHRLAPAKRPAARSRAAKSRKKN"
     gene            20895..21989
                     /gene="lpxD"
                     /locus_tag="VFH05_00295"
     CDS             20895..21989
                     /gene="lpxD"
                     /locus_tag="VFH05_00295"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET6676684.1"
                     /translation="MGSLSTPTTLTLSQIHDLVGGTLHGNGASVLSSLASLDDATPQA
                     LAFISNDKAAKVPGGIKAGGLLVHRHLSEIDVPQIVVPNPLLAFAQVAQTFFIRPSAP
                     RGIAADVSKGTGVTIGTDPSIWPFVTLGDRVTIGARVTLYPGVFVGADCTVGDDSVLY
                     PNVVVREGCSIGARAIIHSGTVIGADGFGYVQHEGRHQKIPQLGGVTIEDDVEVGANV
                     TIDRATLGRTIIKQGTKIDNLVQIAHNVTVGEHDIIVAQVGIAGSTTIGHHVMIGGQA
                     GLADHLTIGDQVMIAARAGVNRNIEPNQIVSGAPIMPHETWMKAQAVIPRLPELRHLV
                     RNLEQRMEKIESRIKSKAAPKGKRSKRGRA"
     gene            complement(22004..22525)
                     /locus_tag="VFH05_00300"
     CDS             complement(22004..22525)
                     /locus_tag="VFH05_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676685.1"
                     /translation="MSKLVKGWRNEDIHAQARHYVRTYILLPSGFLGLMCVLGGIGTL
                     GYQLIASATYTMMTFMTSSALLLLGGLCGWLQTRYHRYLLETVPDVFAARMRSAVQRS
                     HRKAKAGSTIPPIVHRGRSLIPVAYVLGCGLLLSASGWAIMQGAMDAVPAILMPWAGF
                     YWSKLFFWRGIVN"
     gene            complement(22774..24858)
                     /locus_tag="VFH05_00305"
     CDS             complement(22774..24858)
                     /locus_tag="VFH05_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6676686.1"
                     /translation="MNQVGGASIHRIEMEERRATDLYRRTEHTLTSVASAIQRQDPLD
                     LKELSALAREIAESIARNDQLVVQALAGPSGSTLITNLINVSILATKVGSGLGYYGEE
                     LQRLTLAGLVHDIGLFAVPQSVVTKSGKLTPEERTLIEQHPELGHQLVKKLGGEWQWL
                     ALVVRQAHERSNGRGYPNRLKARDIHEFAQIIGAVDVFDALVTPRPYRRRFFPHEAVR
                     ELLVVERSAFPREIIKALVEQLSAYPLGTLVQLTTGEVGSVVGINVQFPLRPIVEVVA
                     DSTPGNGADKREIDLSLSPLVSIIETVESPDVGRVSFRSDALADRRSKTLPTVSDQFT
                     SLLENLDALASAIQGVVESRTSSASDTGPTETRADNGSTGRSTEQDVLDASFEKEVIG
                     LFALEAHEWLAQIHSALKRLSEGSSGTTRSKLSGIMLQGLSNLARSAATVHLTGIEDL
                     ALGLLPVLQDVGRHEPRAMAAGFASLKSGLDRIAAAVRQAEGRPPADERPEFSGTEVT
                     KTSSSVAPLRMTTGASLLSALRDLQHVRSRSVQPTRDVLDGVIRRAEQEDGEVTVDGI
                     RRILEELDRMDERFVDEVGRRIPVVTRILAELQKDDSDFVTSSQLTPILDQVEALHDA
                     SETVQAGMITMFLQGLRAFLMVAAYRKTPTLAKRLAAVESRIQALVPMAEQWVSIGRV
                     ERAVIAEILPVG"
     gene            complement(24920..25849)
                     /locus_tag="VFH05_00310"
     CDS             complement(24920..25849)
                     /locus_tag="VFH05_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676687.1"
                     /translation="MSGDTSEEFQNELVELFVQEAHEWLQNIHVALDEIQQGPPPERH
                     AVLINTLTAGVTNLGGSAATINLPNVEQASFAALPFIEAIKDPTKSFSVQDFLSLCKQ
                     LGQIHTALTNATGVSFDAEAESETVEAAPGLSPTEFVETLRKLQSTQETTGPLERNLI
                     KTMIDQMEGQVQAGVQHVGVDVIQGYLDRMSESEEAFVQVVDEQLPTLTAQLSSLADP
                     ASLPGGVLEASIREVTQLRAEAQQVNVTPAMTFFSGLHSLLSVLAQHHMNLAAPRVQA
                     ITDRLGVVRKLIHQWVERGRSERAAIGQALPAR"
     gene            complement(25996..27312)
                     /locus_tag="VFH05_00315"
     CDS             complement(25996..27312)
                     /locus_tag="VFH05_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6676688.1"
                     /translation="MPKILVADDSIAVRKVAERLLTEAGLGVTLAANGEEALALLARE
                     VPDLIVSDVIMPDKSGYEVCAFVRGQAALANTPVLLISGIVNDEVTKQAESCHADGVL
                     KKPFQGTSLKDRVLELLGKKQSAPAPAPAKAAATSAAPTAPANAQVLDEQGPAARQAI
                     EKLKQIEEELQNERRQTSLLTQRISQLETQTGRIKELEAQSGRLKELEVQAARMKELE
                     VQSGRMRELEAMLVAEREQTAQAKKAVTELTANLQRLKELEPLLKHEREQSAQFKKQV
                     ADLQSQAARVSELEGALQKERALAAQQTQRIAEMQKSVGRVQELEAALNAERQAATQL
                     VQQMTTMEKAVNRSQELAQQFAREQQRVEELTRRLADQETGGTKAKEVQQLLETERER
                     NAVLARRVSETEMAAQQATRRFEEMAKKLGEIAGLASQLGSGSKRA"
     gene            complement(27337..27801)
                     /locus_tag="VFH05_00320"
     CDS             complement(27337..27801)
                     /locus_tag="VFH05_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6676689.1"
                     /translation="MLRAVQKHDQVSAKRWWNVVVFSVGGKKLAARTEDVGGIAPWIE
                     PLPVPSRTPFVGAMLKRDKDVMPVYDLGGRLSRHPEGDTLLCLVARHVDGPMAICIDG
                     VIPSLHNVDVAQILPSKRLDLETLGSFSEEGQDIEIVALQRLGRNRQDYVGS"
     gene            complement(27809..28297)
                     /locus_tag="VFH05_00325"
     CDS             complement(27809..28297)
                     /locus_tag="VFH05_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6676690.1"
                     /translation="MSLRGHHIATTAVVQTARFLVVRFDRILCALPSQGVRGVLTPAE
                     AGTGSQVTAVGITYQDIDLASRLGVTLDPANPDMRVVLYSNGQTNGAMRVEAVIGMIE
                     VEREECRPLPSHFQHEERTWISGTTMCKDELVLILTPEWVLGELGEVVAGATGQRALS
                     AS"
     gene            complement(28349..31741)
                     /locus_tag="VFH05_00330"
     CDS             complement(28349..31741)
                     /locus_tag="VFH05_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET6676691.1"
                     /translation="MSSELDRQGLLDIFIMEAAEALAALSAAVNPPGDSVPSASELQD
                     QYVWAHKVRGASGIYGYTGLSLLGELLESTLEQSTSIDPALWLKTVGALRGMVEAFQS
                     QLDVIKQGGQEDPSVSAHWKKEVMTLLPASSDASSEDGSKELGTDYLVPTIDAEVLSY
                     FAPEADEYLQTLEVLTNRLKENPGDAETIQALYRTAHTLKGSAHTIGFKVIGDIAHPI
                     ESCMIAVRDGHIPVSTALLATIGDAAALIRLVMRRDESHLAQLRQGVPGVTQTLQQIQ
                     SGQPVGAPAPAIAHAQERPVAQVATAVLEPSVVTDQPAAAVPAPSLTDEYLMPQLDPE
                     VLSYFAPEAQEYLESLEAQLLRLEKEPQNPDLINQLFRTAHTLKGSAYTVGFQSIGDL
                     THYIEDFMGAVRESRIKVLPGHTDVLLRSVDVVRLLMRRDPTLTGTVRQRFAAAMTAL
                     KQLEQTKTVETVVKAAPVQPMPEPVVEQAAQQPEPEQGKVRDAAVEEREVIRVSRDRL
                     ERLLNLVGELVIGRGRLEQRLSVLEQLSQQVLACKVRLTDSVRSFEEKHTFTLPTSAG
                     GTADQARTGSAAAAFQGLSDFGSLEFDKYDDFNILARRISEVTADISESMTQLSGSIR
                     RSHEDMSQLQQLTLGMRDEIARARMVPIGTPFTRFRRATREMARATGKEVALVTSGEH
                     TEVDTGVVERLVDPLVHLVRNAVYHGIEPAALRVSKGKPAAGTIYLHASHRGNAVLIE
                     VEDDGAGLDIDKIRAKAVERGLVRSDIAKNLPEAEAIKFIFLPGFSTAEQVGDQAGRG
                     VGMDVVKRVIESMNGHIDVESIRGVGTKFTLHLPLTLLIATALMVRSGEERYGIPLPS
                     VREVTMLTAGSLQQMGERSMLHLGDEAVDVQPLQQLMYRNGSARVEVGKPVVIVRTGT
                     GPVGLAVDELLGRQEIVIKPIGALKSLERSCFGGATIDPEGRVVLVLDPARLVSGGEQ
                     AGSQLETLNETTAGTDTETPFQEPMPQPTVEQKILLIDDSLSIRKFVGRMLETAGYEV
                     DTAVDGEEGLRKASAKNYRLILTDLEMPKLNGYEVIQALRSRSQTQQTPIIVMTTRAG
                     DKHRQMALNVGASSYIAKPVEERALIQELERWIGQESPARKA"
     gene            complement(31793..33667)
                     /locus_tag="VFH05_00335"
     CDS             complement(31793..33667)
                     /locus_tag="VFH05_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET6676692.1"
                     /translation="MLRLNFLKWFENLKTQAKLLTVFGLVGVIIAAMAGVGIVTNSRL
                     ATQAETVYIDYTIPLTEFNQLLFNVNRYHEALVDVSRTPRASDFEIELKDIAPYRAEV
                     ERLLASYEKTTLRTSSTGRDEAKDLAELKAAVTQFFNEGDAAITTIKESFENKGLTSS
                     QALAMRELGALAITVSMSPAYEKVTQRHAEQIKTMSEVAKDLSEEAKGMAANATMILV
                     VGGIAAVLLGLALGYWVAHKLSLGIGQVASVAQQAASGNYQARAKIASKDELGQMATS
                     FNTMLDRITALVTSEGERDEMQKRLMNFLVLVSDVGKGDLTKRGEVTADMFGNLADGF
                     NLMIARFGQLLKQVREAAERVNKSAGTLRDSAGQMAGTAKHQADESAKTLSAVEQLAS
                     QMRQVAETAGASSESAKQVLQATERGRVAVQETVQDMQSIRSAVQRMSKQVKGLGDRS
                     LEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESSTQATRE
                     IADLVKVIQSETQDAVVAMEQETQAVEAGSASALRTGEVFNEISGIAQRSAELAQTIA
                     SSAATQTASTDQVGRSIKDFTGGAVATQKATDAARLTVEDMAKLADGLTASVAQFKLA
                     "
     gene            complement(33739..34260)
                     /locus_tag="VFH05_00340"
     CDS             complement(33739..34260)
                     /locus_tag="VFH05_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET6676693.1"
                     /translation="MSAPQGATSVATSHADAIVASQGSAAPTSGTSTIRAAIVSLGGE
                     MFSIDLQNVREVFVVESVTPVPGMPSGLIGVTNLRGSVVPLIDLRQMIGVAAETALKY
                     AVVVKHGNWQVGVLVDTVPEIRTLSKDQFLPAPVGTGDGVNPFVSTVVKLEDRLRGVL
                     ETSAILSHFEGTG"
     gene            complement(34266..34628)
                     /locus_tag="VFH05_00345"
     CDS             complement(34266..34628)
                     /locus_tag="VFH05_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6676694.1"
                     /translation="MSKILVVDDSYAELQVIEGVLKGANHTVVSLPNTEKLEDKVVGE
                     KPDLIVLDVVMPGRNGFQACRDLKNDDRTKNIPIILCTSKGNESDKFWGQQQGANAHV
                     VKPFKGDELLAAVKRVLG"
     gene            complement(34672..35409)
                     /locus_tag="VFH05_00350"
     CDS             complement(34672..35409)
                     /locus_tag="VFH05_00350"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6676695.1"
                     /translation="MKATWFNWRMAGGIAAAALMFNASTVLAAGEAEVAEHLIDLVKI
                     GRGVMSEQMGNINDASKSNKGFTGDYMAAQVIERFKKKTKIDLRIPNAVPQAGLYLAL
                     VEAEKDVVDEAQPVINRAGIGFKGFIPAVFARKTGENFYKKTGVKLKLTGIDYRNVSN
                     RPDDFEAEVLRMFSDPRHPKGQTYVRNTMLDGRPVLRMMDPEYAAASCLSCHGAPKGG
                     KDITGGHKEGWKEGDLAGAISVVLPLK"
     gene            complement(35850..36584)
                     /locus_tag="VFH05_00355"
     CDS             complement(35850..36584)
                     /locus_tag="VFH05_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HET6676696.1"
                     /translation="MRRQHTKREGVKYRVCGNTLVIDLAMRYRIVSSAPRGGGIVRGR
                     SILNHQVPANPVMPGGRSVEEWSDPARYLGRLARSVRAAMPVVGLMTAVPMTQLVVDR
                     EQSGSLLLECFCTVGVTNAVRAGEPVRSRLQRTLDRDQAGTINIILVTNATLTIPALI
                     GAVQVATESKTATLIKHKVPSAYEKAALATGTGTDAVVLASSLAGDHKLQYSGTHTEM
                     GAMIGRLVGRCVERGLRRVAGRNKAL"
     gene            complement(36586..37689)
                     /gene="cobD"
                     /locus_tag="VFH05_00360"
     CDS             complement(36586..37689)
                     /gene="cobD"
                     /locus_tag="VFH05_00360"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HET6676697.1"
                     /translation="MALKNALVHGGNVYAAARELRRPIGSLLDFSASINPLGPSPQVI
                     RALATAAPLIQHYPDPDCVSVKQSIARRWKISPDRLVIGNGSSELIDVIPRALGIRSA
                     LIVGPTYGEYARAVARAGGRYQMVLARRNDEYRPPLERVTHRVQVQRSGRRAIDAVFI
                     CHPNSPTGRPGDLQQLDDLFQAAERGRIWVVLDESFIDYCDALTCVPRLQAYPRLIIL
                     RSFTKFYGMPGLRIGYSLSSRSVAAILKDRQPPWSVNTVAQRAAEAAIADRRHARRCL
                     AYVGRERARMTKQLAALAGVTVIPSSTNFLFAELPPSCGAGLMTAALRRRGMLIRDCS
                     SVEGCGLGSIRIAVRTKGENDRLLAALAQVLEG"
     gene            complement(37680..38633)
                     /gene="cbiB"
                     /locus_tag="VFH05_00365"
     CDS             complement(37680..38633)
                     /gene="cbiB"
                     /locus_tag="VFH05_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HET6676698.1"
                     /translation="MTPLDLLAACIVDAVIGDPPAWPHPVRWMGRLITRVEKWTRRFC
                     VGSLSLRVTGFALALGLPSLAFCAGWVAIATGTAIHDWIGRGIGIWLASTVLAWRDLV
                     DHVGAVSHALQTGTLDGARHSVSLIVGRDTDRLSEPEIVRATVETIAESASDGIIAPV
                     FFLVVGGPPLALAYKAVSTLDSMIGHRDAAYEDFGWGSAKLDDVVNWIPARLTGWLIA
                     LAAGAVCWNTRSVHRSGALLLRDGHKHPSPNSGRPEAAMAGALGVQLGGTNFYAGLRQ
                     DRPLLGDPQEPLLAAHITRAQHIMTVAYLIAIAACLGFLWH"
     gene            complement(38630..39394)
                     /locus_tag="VFH05_00370"
     CDS             complement(38630..39394)
                     /locus_tag="VFH05_00370"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET6676699.1"
                     /translation="MTTSLRPFIFAWQFLTAVPLSRDLHDPAPHELAGSMLWYPCVGI
                     LLGAFMVMTDMLLARWLSREVVAALLILLLVAMTRGLHQDGLADTLDGLAGGRVPTDR
                     LAIMRDPRIGAIGATGLFVSLILRYSALVSLPEPLKFPALLCAPAIGRWAMVVGAYGA
                     SYARAEGGLAAPFLAHLSAWHVLGATVLVASLMVWSFGFAAALTILLIGGIVSRLATL
                     CCRSYFGGITGDTLGATNEVIEISFLLSMPFLTYLP"
     gene            complement(39391..>40088)
                     /locus_tag="VFH05_00375"
     CDS             complement(39391..>40088)
                     /locus_tag="VFH05_00375"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET6676700.1"
                     /translation="SVAGLINRKLLMGTRNMVQGEAMDRRTVEEALEIGIELASEAVK
                     EGVTLIGTGEMGIGNTTASAAITAVITGASVEQVTGLGTGIDELGRTRKVSVIERALA
                     RHQPNADDPIDVLTKIGGLEIAGMAGLMLGAAAARIPVVLDGFIAGAAALIAGGLQPR
                     SVQYMIASHRSVERGHRLVLETLGLEPLFDLDLRLGEGTGACLGMSLVQAAVKVLTEM
                     ATFSEAGVSERNQ"
BASE COUNT         7839 a        12459 c        11529 g         8261 t
ORIGIN      
        1 gcaccgcctt tctgaaaatc tggttggtcc ggtgtgccgc ggtcggcgag caagagggat
       61 agactagacc gcccgtctca ggtttccatc atctccaacc gcgcatcgaa ctcgtgtccg
      121 cacgatatgc aacgaatgca atcggattct actgtaaatt cgtccgaccg gatccccatg
      181 cctccgcagt caggacagcg ggccagatgc acacgttggt cgtcgagtgt ttcgtactga
      241 ctgccgcgga aacaatagat gggtttgccg tccaagagcc acgcattccc cgcgttatcg
      301 acgacgggga gcgccaggta atgatggttg acgtagtctt cgatgtcccg gcggctggca
      361 aagccatcct tgatacattt tcccgtcgcg gcttcatata aagcctgatc tttgataatt
      421 gctttggtag gtcccatgag ctttcctcct gctttagaga gttcttgctc ttctgcgtac
      481 atttctcgcg gtgccgaacg cttcgagcct cgttcccacg agggcacgaa ttcaaacgac
      541 gccggagtaa taccgcagaa tgttggacac ggaaatcacg ccgatgatct ccccgtcctg
      601 ggcgacagcg agatgtctcg tggcctgctc cttcatcaac cgcaccgcct cgatgagcgg
      661 ccggtctcca tgaatcgcta cgagcggctt ccgcacacag gactgtactg acgtggtgtt
      721 ggcgtctagg ccattcgcga cgacgtcacg ggccaacgcg gagtcggtga tgaatccgac
      781 gtaagtttgg ctgtcagtca cgaggagcga gccggttttc catttctgca tcagacggcc
      841 ggcttcccgc aaggtggcgg tttgcgggac actacgcaaa tccgatgaca tgtgctcggc
      901 aacctgaggg ccagtcagca tgtgtttgcc tgattggagt ttcttcggtt cgtcggcgcg
      961 gcgagcttcc agttctgaga cgcagctttc caggacgcga gtgcgctgga gaagactttg
     1021 gcgctcgcta tcgaggccga tgctttccgg cgcttcctcc gtcatattga cgagtcccgc
     1081 ggcctcgcct aactgcgcat actggtaggc atcgagcagc gcagatgttt cgccgagcaa
     1141 ttcggcaatc aagcgttccg aatccgaatc gaattcttca agcgtgcggc tggccttgcc
     1201 cttcttcaca tagacggcca gctccgcaag gtgtttgcgc atgcgctcga tgctgcggtc
     1261 gagggacggt cgcggtttct tggaagcgat gcgtgtcgac gccatggcac ctccagtcga
     1321 ttgagggggc aaggaaacga atcttacctg tacccgctca gcggaggcaa gccgcgaatt
     1381 catcggtcga ttgcctagcg gcccgcacac cgctgacctc cgaggccgtc gggcgcacag
     1441 cggatagagc cggaataatc cggcgccaca ggcgcaggca cggtataggc tggcacagga
     1501 cggtccggaa cttccggcct ctgagcaggc gacggagacc aaggaagact ggctggggca
     1561 tcgagatgcc ctgccacgat ggatgacgag gaaggagcag gcgtcggatg aagcaacggc
     1621 tcgctgaccg gtccggattc agacgcagga gccgcccgag acgaaggatg ggacggcttc
     1681 gccacagagg gagctttgcg ggtttcctga tcggccagga ttgcgcggat cgccttgaga
     1741 ttctcctcaa catcgtcctg agacgagata tccagtttct gaaccggttc gccaaccatg
     1801 ataatcccgc agccggacca caagcagaga atccacagcg atgtgactaa gacacgcatg
     1861 gggaacacct acctcttttc cttccacccc tccaatcgag caaggatgga acggctgcac
     1921 gcgatttgtg ccgagactcg ggcaccgtag tgctgagccg atgcggtcgt gctgcacgac
     1981 ggcgagcact gttcgacggt ccgtttaccg gagcgaacgg accgagttgc acagccgagc
     2041 aaggcgaagc attgtagggt cgaggcgaga cacgcgagcg tggcagcgat cgatccagcg
     2101 ttctctcgtt catctgcatc accttgttac tcgcgaaaca cccgcacccc gaccttgcgc
     2161 cctttgattc tcgatcgccc cagtacttcg acaatgccct tcgcgaggga ttcaggcaca
     2221 tcgacgagcg aaaatcgctc ttcgatctga atcgcaccga tgaggttcga attgatgtgc
     2281 gcttcgttgg cgatggcccc gaccagatca ccggccctga tgccggcgtc gcgccccgct
     2341 cccacgtaga ggcgcgtcat gccggcttca cggccacgaa gtgttcggtt cttcgcggga
     2401 ggcatggcct gtcggccatg tcggttccgt gatgcgccat cccgatggag gccctcacgc
     2461 tgcgctgagg gatgccactc cgcctgccgc atcggtaaag ctggaatctc ctgctcaggc
     2521 cgctctcctc cctcggcgcg gaacagcaat ttcattgcgg ccgccccgat cgtggccaga
     2581 tccgtcgcct cggccatggc cttgacgact ccgcgaaagc cgtcgagatc tcctgcccgg
     2641 atcgcctcct gaacggcgct tcgcgtacgc tccaatcgtt tggccacgac atcggccacc
     2701 gacggcaccg gtgtcacgac cacttttccc ttcgtatgtt gttcgatgct tcgcaacagc
     2761 cgttgttccc gcggatcgag aatcgtaatc gcagtccctt cgcgtcctgc ccgtcccgtc
     2821 cgtccgatgc ggtgcacata gacttccgct gatgagggca gatcatagtt gatcacatgt
     2881 gagacctgcg ggatgtcgag tccgcgggcg gcgacatccg ttgccacgag cagttcggtc
     2941 tgcccagtcc ggaaggcctg catcacccga tcccgctgga cctggctcaa ccccccatgg
     3001 attgcttcca cccggtgtcc acgggcgctc agcatgagcg tgacctcgtc gacttcgaca
     3061 cgggtgcggc aaaacaccag cgccgacttg ggcgcggccg tctcaatgat gcgtgcgagc
     3121 gtcgcccctc gatatggtcg tgtgacgatg taggcggcct gctgcacccg cggtgcggaa
     3181 cccgctttga cggcttcctt cgcgatcgta atttctaccg gctgctgcag gtgccggtgc
     3241 gcgatcgatt ggattcgcgg cggcatcgtg gctgaaaata acgcggtctg tttggccgca
     3301 ggcgtgtgct caaggatcgc gtcgagatct tccgcaaatc ccatgtcgag catctcgtcc
     3361 gcttcatcca gcacaacgat ctgcacggct tcgagcttta acgtgtggcg tcgaatatga
     3421 tcgagcgcgc ggcctggtgt ggcaacgatc acatcgacgc cgcgtttaag cgcatgcatt
     3481 tggggaccaa tcgcttgccc cccgtagagc ggcaggacac tggtccggag ttctttccca
     3541 tagcgcccga cggcctcgcc gacctgaatc gccagttctc gggtgggcac cagaatgatc
     3601 gccgacggct ttgtttttgg actgtgagca atgcgttgca gcaagggcag tgtaaaggcg
     3661 gcggtcttgc ccgtcccggt ggctgcctgt cccaacacgt ctttgcccgc aagtaaaggt
     3721 gggatcgctt cccgttgaat cggcgtcggc tcctcgtagc ctaaggcctc cagtgttgcg
     3781 agcaacctcg cttcgagccc gagcgccgca aagccgggag aaaagccgtg aacggccgtc
     3841 gcctccggcg catgggtgtg gtcatgtttc atcaaaaatc tcttttcacg acaagagggg
     3901 gctttttcac gcttctcatg agggttgccc gatcggtctc aaacgtgcgc gcgtcgacga
     3961 gcacctcccg cagtccaata ttagatcatt ctggtgtgcg cgttgcgcga gcacaaaggg
     4021 accgacgggc aacgcgtact ctcttcatcc cagttgcttg ggccagagcg actccaattc
     4081 ggggaacttt tccctgtagt cggcatggct ggctcgcagc actttaagcg cctcgtcacg
     4141 tccatagatg ccggtgattt ccactttatg ttgcagggac cccggcgcct gcttgtaggt
     4201 aaggaaataa tgttggagcc gctccatcaa tggcgcagga caatcaccga tgtccgtaaa
     4261 acttccatag gccgcatcgt ccttcatgac ggcgatgatc ttatcgtccg cttcgccgcc
     4321 gtctgccaag ctgaatccgc cgatgggaat cgcggtcagc aaaatgtcgc tgtgaggaat
     4381 ggtcttttcc gtcagcacac agatatccag cggatcaccg tcgccgacca ttccttttcg
     4441 cttggctcgt ttggaaaaga tggcggcgac tttgttcccg cagaaggttt ggggaacgag
     4501 accataatag acgggacaat agttggaaaa ccgttgcggg cgatccacct ttaagaatcc
     4561 gctgtcctta tcaacttcat acttcacggt atccgtcggc acgatctcga tgtacgccgt
     4621 cacgacgtcc ggcgccttct ccccgatcga cactccatgc caagggtgtg ccttgaacat
     4681 cagacccatc aaccgttgaa tcggatcccc cagtgaacgc gccatcaatt cctcctactc
     4741 aaggtccgag aacgcttcga aggcaatctc gtgacggcga taaaccattt tggcttcatg
     4801 cgtcggaagt ccgtcctcac atgacgcgta agcgacgggc tcgagtcgag gacatacatc
     4861 gagtcatcct ttacacacac cacccaatgc caacacgctc ttcccttgat gcgacgccac
     4921 ttgatcgcca ggagagagcg cttgggcaac gctggccagg accggaacgg ccgctctctg
     4981 gaggaggcgt aaaaacgata ccgcttcagc aagcggcgca catgagtggt ctcggaccag
     5041 aggcggtgat cctcggcgct gattcccaaa cgacccgctg cttgccgtgc cgcctggtaa
     5101 ctcacaccgg ccagcgcagc cacgctggcg atcccacagc ccgttcgctc cagctggatg
     5161 accggcttca tcacaacagt accttcaact tgcggccgcc cgagatcgcg aatccatgac
     5221 gctgataaaa tgccagcgtc cgatcgaact gcggcaaggg aggcgtggtg acttcaaggc
     5281 gccgccatcc tctggcttgt ccgacttttt ttgattcggc aattaatgcc gtccctattc
     5341 cccgcgaacg atgcgacgga cggacataga attccggaat cgtgccgtac gctccctccg
     5401 tatacaacgc ataactttga tacagggcca ggaatcccac agcagcttca ccgtctctcg
     5461 caagcaagac gaggtaggag ccgtcaatga gccaggcacg agcgcgagct tcggtgtcac
     5521 ggtgatgaaa gttgaaggcc ttctcactca cagccgacat gatttcctgg agaagctctc
     5581 caaccatggc cgcgatgacc tccgcatctt ccggccgagc gagatctata tgagcggtca
     5641 cgggagtttt tctccatttc ctgtttgcct gtccgagcaa tgctccttcc aaccttgctc
     5701 gttcgtcctt tcctccaggg tcgatcgata tccgttgttg aggtgggaga cagattgctc
     5761 ccacgtgcgc gcgtgaacga gcacctcccg caagcgttcc gcgagcacag ggagcacctg
     5821 tctaccgatt cattctgctg gttcctcaat cggccccgcc cactcaccct tggccaatcg
     5881 gacctcttga tagccgccgt caggccactg gaattgtccc gcctcatcga cttgcacgat
     5941 gaagggatcg gcctcatggg cctcaccgcg gaaccaatac catcctgctc ccagtggctt
     6001 ggtcctcgtc cattgataca ccgtcattga gttccatcct tacgcgccgc ttgtccacac
     6061 actcgtcctg cgggaacagt acgttccttc tcaccgcagg catctgttac agtccgaccg
     6121 tgacgaaccc tagcatgaac cgactgccaa tagaagatgt gctgccggtc ttgcgcgata
     6181 cgttgacgca gggcagcaat gccgtcttga gcgcgccacc gggtgccggc aaaaccaccc
     6241 gcgtgccctt agccttgctg gatgcgccgt ggctgaccgg aaaaaccatc ctcatgctgg
     6301 agccgagacg gctcgcggcc agagccgccg cccatcgcat ggcctcctta ttgggagaat
     6361 cggttggctc gaccgtcggt tatcggatga gactggatag caaaattgga ccggtcactc
     6421 gtattgaggt ggtcaccgag ggcattctca cgcggctctt gctcgaccag ccggaacttg
     6481 accggtatgg cgcgattctc ttcgatgagt tccacgaacg gagcctgcag gccgatgttg
     6541 gtctggcgct ctgccttgaa acccagcggc ttgttcgtcc ggatctccgg ctgctggtga
     6601 tgtccgcgac cttggattgc gggcccgtga gcgagctcct tggcgatgca ccgatcatca
     6661 tttcggaggg ccggatgttc gccgtcgaaa cgcgctatct cacccgcccg cacaccggcc
     6721 cgctcgaggc ggcagtgact caggccatcc gtcgatccct cactcaggac cagggcagcg
     6781 tcctcgtctt cctaccgggg atggcggaaa ttcgccgcgt cgaacgacag ctgcttgatt
     6841 ccaatcccgg accaggcgtc gtgatcgccc cgctccacgg cgatttgcca caatctgcgc
     6901 aggacaaggc gattgtgccg gcggagtccg gcacgagaaa agtcgtatta gcgacgtcta
     6961 tcgccgaaac cagcttgacg atcgacggcg tccgagtcgt gatcgatgcc ggtcaattgc
     7021 gagtcccccg gttcgaccct cgttcgggat tgacgcggtt ggagaccgtc cgcgtgacgc
     7081 aggattcggc cgaccagcgc aaaggcaggg ccggccggct ggagtcgggc atctgttatc
     7141 gcctgtggac ggaacgagaa cacctgtcac tcgcaccacg aaaaactccc gacatcttgg
     7201 aggccgatct cgctccgatg gtcctggacc tcgctcaatg gggagagacc gacccttccc
     7261 gcttacgctg gctcaccctg cctccttcgg gcgcggtcgc acaagcgaag gacttgttga
     7321 ttcgtctggg cgcactggat atcagtggcc gactgacccc tcatggcagg caaatggccg
     7381 gcctgccgct gcatccccgg ctctcacaca tgctgctcaa gtccctgacg ctgggctgtt
     7441 ccaacctggc gtgcgagttg gcggctctct tgagcgaacg tgacttctta cgaggaccct
     7501 ccgccacccg gcagacagac cttcggctac ggatcgatgt actccacgac atcaagaagc
     7561 atgtcgccag cggcacgatt gatcgaggaa caatcgagcg gattacacga accgcccgcc
     7621 aatggaaaac gcagctagct cgtgtgcacc ggctgccggg aggcgggtct tcggggacgg
     7681 acgccgtcgg cctgctgctt gctctcgcct atcccgatcg gattgcgcag cggcaaccgg
     7741 gccatgaagc ccgatacgtt ctggctaatg gtcgtggcgc cctttttgcg tatcccgacc
     7801 cgctcgcctc tgaaccgttt ctcgtcatcg cagcattgga cgccggcaca ccttgggccc
     7861 gcatcgatct cgctgcgccg ctgtcggcca aggagatcga gcagctctat gccggtcaaa
     7921 tcgaggaaac cgaagcgatc tcatgggatg accgaacccg gtcggtgcgc gcctcgcgac
     7981 aacgccgttt cggcgccctc atcctgtctg aggaggcgct ctcgaaggcg gatcctgcct
     8041 tgatcaccaa ggccttgctc gacggccttc gccgtgctgg cctggcaacg ctcgcgtgga
     8101 cgccggaact ccaggaatgg cgcaggagaa tccagttctt gcgtggcctg ggcaaccgcg
     8161 acgtctcagc gtcgcgaggg cgggagggtg agattggcaa ccgcgacgtc tcaacgtcgc
     8221 gagggcgaga gggtgagatt gacaaccgcg acgtctcagc gtcgcgaggg cgggagggtg
     8281 agatcgacaa ccgcgacgtc tcagcgtcgc gagggcggga gggtaagatt gacaaccgcg
     8341 acgtcccggc gtcgcgcggg agggagagtg aaacggacag cgacatcacg tcgacaaggg
     8401 aaggaaaggg tgcgaatctg acatggccgg atttgtcgga cgagcgattg ctcaacacac
     8461 tggaagactg gctgggtcct tatcttcacg ggattaccac gttggagcgc gtgcaacgca
     8521 tggatctgac gcaaccgctc catgcgctcc tgacctggaa tcaacagcgc gacttagacc
     8581 ggttagcgcc ttcgcatctc accgttccca gcggatcgac aatcaggctc gactacgaga
     8641 cgccggatct tcctgtcctg gccgtccgcc tccaggaaat gttcggctgt agagagacac
     8701 ctcggatcgc gaacaatacg gttcccgtgc tcctgcatct cttgtcgcct gcaaagaggc
     8761 cggtgcaggt gacacgtgat ctcgccagtt tctgggtcaa cggctatccc gcagtccgca
     8821 aagaactgcg cggccgttat ccgaaacatc attggcccga tgatccgctt atcgcagttc
     8881 cgacagccaa aacaaagcgg cgcacgtagc atcgcccttt cgtaggatag acccaggacg
     8941 ctgtggcatc ttatcgtgcg aggcatggtc caatgcctcc cttttctcct gtccctggcc
     9001 gtgatcgttc ccatcgcgat cggtccaacg atcgccctgg ccgacgtccc ggcgcttgcg
     9061 cctcctccac cctcagcgtc cttcgacgat gtggacgaca tcacggttca ggccgaagtc
     9121 accgggcagg cactcgcctt tctcaccaga ctgcaacggc attgggggga gagtgcagac
     9181 cgggtgagga cgctcatcgc gggcaccatc gaacgcgatc aagagggcac gctcatctac
     9241 ctgcgcaatt ttcgcgatca tggcgtgctc gagggctatc agttccggaa ggggtcgctg
     9301 acgtgtggtc gttatgtgat ggtgcaacgc ccgctcaacg gcttgaacga attcatcggc
     9361 tactatgctt cactcaaggg ggccttgcat tcgaagtatg gagaccctga attcgatcga
     9421 gtcgtgtggg acaatgattt gtaccgaccg cttcccgact attggggcgt tgccgtgatg
     9481 atcggccacc tcagattcca tgcctcctgg aacacagttg atgggacgat tgcactggaa
     9541 ctgacgggga accgccatag cagactctcg ctggaatcct gcaccacgcg tgaagaagcc
     9601 caaacttagc cgtcgtaaca ggtcttcaaa aaggccggct ttttcagcca cctgctaggt
     9661 gggaatgtcg cggacaccgt catacgaagt tttcgattgg tgggtcagct tgacgacgtc
     9721 gccgtccttc acaggagagt cttcgctccc aaccttcttg ttcaccgcca caagcacttc
     9781 acgctgctgc atgaacggag cgacggcgcc taattcagtt ggatacgcat cgatcagctt
     9841 cttgaccgtc gtcacctgag gcacatcaag ctcaacctca tgcgtgccaa cggcgtcacg
     9901 caacgtcaaa ccaaatacga gcacttttac catgcggcgt acactccttg atcgacttct
     9961 cgttgtccgt ggtccctgag tatcatcagt tgaactccag cgtgttcgct cacgcttgcc
    10021 gcgcgtgaca gcgcagacaa tccggccgct tgggatggcg cgccggcaag ggcttcaccc
    10081 cgtcgctctt gtgacacccg acacactgtt gctccgactt ggctgcctga tgctcggcag
    10141 tccgaggaac catcggattg cggtcttttg gcgtcggcag aaaggagacg ccagccacca
    10201 ccgtgacggc gaatacgacg aacagccaat cagccctccg caatctcatg gatgaggctc
    10261 gccggtttgc cgctcccctg cctcataact ctcgcgcagg acttgcgcca catgcttcac
    10321 tgccacgtct gccggcaagc cggtcttgag ctgaatcatg caggccggac aactggtcgc
    10381 cacgacgccg gcgccgctct gagcgaccgc ttggcgtttg cgcgacacaa tccgctgcga
    10441 tgtctcgaaa tccttgacca ggtaggtccc ggctccgccc gcacagcgat ctgcatccgg
    10501 catctcgaca tactgcacgc ctggaagatt ctgcaaaatc gcacgaggtt ctttcgtcac
    10561 ccccgccgct cgaaggtgac acgaggaatg gtacgtcacg gtgcgcgcgg cgccgacggt
    10621 agccatgggc ggccggaccg gtgattgcgc gacgaattca gaaatgtgcc tcactcgacg
    10681 ggccatggcc tcggcagcag actgttcagg ttcgcccgca aaaagtttgg gatagtcttt
    10741 gagcatcaac gtacaagagg cgcagccggt gacgatcgtc tcgtagccgg tgaacgatcg
    10801 caaattaatc cgcgcgccat ctttcgccaa ttcccgatgc ccgtaggttt caatcggcgt
    10861 tcccgagcac cgttgcgcag gcagatccgg ctcgacgcca tgcttgcgta ggaccgcgat
    10921 gacggcatcc ccgacgccgt cgtcgaaata attggccgcg cagccatgga aataggccac
    10981 ggctgagcgg cggcgctgct ccgggtccag tgccaactgg ggatatcggt cgcgcaaatg
    11041 ccgactggca aatttcggca tagcgatggc cggcgagaat ctcgcgctct ccgccaaacg
    11101 gctgagaatc ggccggctca cgcggtccaa aagtcgcctg atcagcggcc gatcccagag
    11161 aggttggaac ctcgccaccc acttgagaaa cgctgcgaac tgacttgtct tggcttggaa
    11221 ccggaagatg gctcccgcaa gtttattcgg gtgctccgcg cgcttgtcca gaatcaactg
    11281 ggagacgtcg acgccggccg ggcaggcggt tcgacaggat ttacagttca aacaggcttc
    11341 caccacccgt ttcgagttta ggtaactgta ttgggcatcc gtcacgatct cgaaccaacc
    11401 ccgcgaactc atatcctcgg agcggaagac atcatacacg ggacagacgg cattgcactt
    11461 cgcacaggtc gcgcaggatt tggacaacct cgtatagtcg atgtgatcgg taaacggcct
    11521 gtcgctgatc ttcacgccgg gattcaacat gccggacggg tcaaaacttc gtttgacctt
    11581 cacgaacaag tcgtacagct ccggcccgaa catcgcctgc acatactcgg cacgcacccg
    11641 accgtcccca tgctctccgc agatggaccc ttcaaaccgg ttcaggacgg tctggtgaat
    11701 ttcatgatag gcctgcacca tcttgtcgaa gtcccgcttg tcgttgacgt cgagcagcgg
    11761 cacgatatgc gcattcccgt tgccgatatg accgaaaatc gcgaccggca cgcgctggct
    11821 gccgaaaaat tcctccagat accggatcaa ctcgctgatc cgttccgcac gcaccaccac
    11881 atcgtcgacg aagttgatcg gcttcttgcg gggatcgaat cgatacaagg tcgggtacag
    11941 ggccttgcga gccttccaca gttgctcgcg ccgctcggga tcgtaggcca cgacaatctc
    12001 ggaggcgagc gggtagtcac cgcagatcgt cgccatccgt tcggcggctt cccgaaggtc
    12061 gaccgccccg ctttccgcct cgagctcggc cagcaacgtg gcggccgccg cagccggtat
    12121 cccatgcgtc gatcggccaa tgagatcgag cgtgttggca tccatcactt ccagagcgct
    12181 tgggataagt gcgagcagcc tcggaacggc atcgccgaca tcctcgaggc gcttgaaatg
    12241 aatcaaggcc gtcagcgtgg cgcgcggctt gggcacgaga ttgatcgtag cctcgctgaa
    12301 tatgcccaat gtcccttcgc tgccgacgaa gaggtgcggc agatgcaggc ggccctgagg
    12361 caagccgtcg gccagtccga agaggttata cccgcagctg tttttactca cggccggccg
    12421 ttttcgcgcg agcaggtccg catgggctcg gattagggac agggtttccc ggatggcggc
    12481 atgagacgac agcacgccgg caagcgccgg atcatcgaga tcgaagtttc tcgcatccag
    12541 ccatgagccg gatgccagac acacgcgcag gctccgcaca ttgtccttca ccgctccgta
    12601 ccgcaaggtg tggggaccgg ccgagttgtt ggccaacata ccgccgagct tgcacatgtc
    12661 gccgctggac ggatcaggac cgaacatcag tccctcgcga gccagctgct tgttgagctc
    12721 ggcgaggata atccctggtt gaacacgggc ccatcgttct tctcggttga cctccaagat
    12781 tcggtgcagg cgggacacat cgagaatgat gcccgatccg atggcggagc cggttaagtt
    12841 ggtcccggcg gcgcgcgccg tgagcggaac actgcggttc atcgcatagc tcacaacacg
    12901 ctgtatatcg tcttcggact ccactaaggc aatggcttgc ggttccatgc gatagatact
    12961 cgcatcgacc gcataggccg tcttggttgg cgcgtcgtcc ttaatttggt ccggcttcag
    13021 aagtcggcgg agatcggccc cgatcgcgcg ggaagtatca ggcatgacca gcatcgcgcg
    13081 gcattctaac agggaggaga aaagttctgc tactcattgg tttcggattc tcgaacaact
    13141 cgttaagata gcgcgccatg gccagcgcaa aacctctcct ctatgtctcc cgactcctgc
    13201 cggatcccgt gatggccgcc gtccgagcgc agtacgacct gttgcacgaa ccgtcgaacg
    13261 gcccggctgc gccagccgcg ttgagacagg gcctgtccca ggccgttgcc gcaatcgtca
    13321 cactcacgga tcgaattgac gccgacacct tggcatcggc gcgcgagtta aaggtcctgg
    13381 ccaactatgc cgtcggctac aataacattg acctgaacgc ggcgcagcgg cggggcgtcg
    13441 cggtcaccaa cacccctgat gtgctcacgg acgcgaccgc cgacctcacc tgggccttgc
    13501 tgttggcggc tgcgcggcgg gttgtggaag gcgaccgact ggtgcgcagc ggacagtgga
    13561 ccggatggac accaacccaa ctactgggcg ccgccgtcgc cgggagcatc gtgggcattg
    13621 tgggaatggg gcggatcggt caggcggtcg cgcggcgggc cgccggcttc cggatgccgg
    13681 tgatctatca ctcgcatcgc gcggttgacg gaccgccagg cgcccactgg gcgtctcgtg
    13741 atcttccgga tctcctccgc gagtctgaca tcgtcacaat ccacgtgccc ttgacgtctg
    13801 agacgcgaca tctgatcggc gctccagaat tcgcactcat gcgccgtacg gccatcttga
    13861 tcaataccgc aagagggccg atcgtcgatg aatcggccct cgtcaccgcc ctgcaagagg
    13921 ggaccattgc cggagccggg ctggatgtct ttgaggacga gccgaagata catccgcatc
    13981 tcgcgtccct gccgcaggtc gtgctattgc cgcatcttgg ttccgcaacc ttggccacaa
    14041 gggtggagat gggcatgatc tgtctcaaga acatcgaggc gattctggct ggacggccgg
    14101 cgcccaaccg cgtggcgtaa gagagcacaa tttgtcgcgg atcaagggaa ggggcgtcgc
    14161 aacctacgtc gcgtaacgaa aggcttgcgc agaagcagag gctggtttcg ggaccgttcg
    14221 gatggccgcc aggcgttgtg gcacgtcgtg aaaggccgga attgcctcga acatcgttgc
    14281 ggcaagctgc cactgctgct gctgctcgta gagcaaccct agctcatagc gtatggcctg
    14341 agccttggca ccctgacaac gtggatcggt caggagccgc tcgagttgtg cgcttgccga
    14401 atcgtatcgt tcctgttcct tcaagcacac cgccgtcatc aggcaggcat cgagaaatac
    14461 ctcgggacct ttcatggcga acgcgaactc ctctttcgcc tcctcgatga gtcccatgtt
    14521 cttataggcc acgccgagcg tgtagtgggt ttcggcatcg acctcatcgg ccggagcagc
    14581 cggcgccatc gttgccgaca gttttgcgac aatcggactg gccggcgcca ggtctttcac
    14641 cttgtcgtag aggtgacgaa tcgggtcgtt cacgacggag tcttgccccg ctgacatgag
    14701 ttcgagggcc cgggcatatt gaagggccgc tccggcccca tcgccttttc gctcgagcac
    14761 ctgaccgagg agttcccgtg ggtgcggatc tgctggctgc gcgctgacga ccgtgccgag
    14821 ccactgttcg gcttgagccc actgccccga ctctgtgtag gcctgaaact gctccgagat
    14881 cgaaagagcc gcttggcctg atgcgaccgg ctcaggagcc tcggccgaaa gactcggcag
    14941 atcatccggc gtggcattct caacggaaaa cacttccggc gagctcggtg ccgatggcgg
    15001 caaccccacg ggtacggcct gccccgccag atgtcggtcc tgggtcggct tgatggccga
    15061 agtcatattc caggcatcgt cgggagtcgc cccttcgatc gaaaagcctc cgttcgccgt
    15121 cgcaggagcg gctgctgcgg cgggctccac ggtcgcctcg ccccgcatca acgctgccag
    15181 ccgcttggcc gtgggactat cgggcgccag cgtctgcacc ttctcgaaaa tttcctcgtg
    15241 caacgtgggc atgcccggtt caggagtctt caagagcact tcgatcgctt tcccatagtg
    15301 ctggatcgcc gactcgatct cgcccttttc ttcgtacagc tcaccttgca actcgagaag
    15361 cggcacggag ttgggctcaa ccgccaaaaa ctctcggatc aacgtctcgg cctgcggata
    15421 gtcctgcgcg cgcaacgccg cccctgcgag aaaccggtat tcaccaaggg cgacctgcaa
    15481 ctgtccttgc tggaggtgca agcgcgctag gagctggcat acctgcggat tgcccggttc
    15541 tttcgtcagc atctgattga ggatggcctc ggcaccggca tattgtttct cttcgatccg
    15601 ccggctggct tccgccaaca aatccgtcgg ctctccggta tgggccgccg gcgtcgatcc
    15661 cggcttcgtc gttcgcggtg gggccgtccc gccggttttt ttgtaatgtt cgatgaactg
    15721 ctgcgcctcg ctgttcgaag gatcgatgcg caacacggat tcgtaggctt ctttggcttc
    15781 ggcataccgt tgttgaccgg agcgctctcg cccaagctgc agatagactc tcgtggcctc
    15841 atcctgcatg ttctcctgca cgcacagttc ggcaacgcgc tgctgggcgt cgagattcga
    15901 aggatcctgg ctcacgattt tcttgtagat atcgagggct tctttggttt tgcgttcctt
    15961 gaggtagtgt ttgcccaacg tcaagtaatc ctggactgcg ctgctcagca gccctctctc
    16021 agcgtttaaa tcgccgagaa accggtacat gtcgtaccgc gacggatcga gtttgaggac
    16081 tttcttaaag gtcgcgatcg ccttgagggt cgctccttcg gcacgaaagg cattggcggc
    16141 ttggataaag gacgcgacgg catcagcggt cgagttgcgt ttcagatgca gttcgccgat
    16201 ggaattatga atactcccgt cgcccggtga ctcggccgag agtttcttcc attcgctgat
    16261 cgcggcgtcg aactgacctt ttgaagcaaa gagctgggcg ctgtgcagga ccttactgcg
    16321 gtcgatgccc aagagaacct ccaatagtga acaaacgcta acagtatcaa tagtttttgt
    16381 caacgaaatg ccctaaacgg tcgcatcaat ccgggtcaaa cacgaaaggc ctgccggagt
    16441 gtttccggca ggcctttctc atgatgcagc ttggagtcgt agacggttag gacgcggcga
    16501 tcgacatttt catgacattg gcagcctgtg gtcccttggc gccttgcacg atatcaaatt
    16561 cgacgttttc tccctccttc aacgacttga atccttcccc ctgaagcgcg gagtaatgca
    16621 cgaacacatc ctctccgcta tccagacgga tgaatccaaa ccctttccga tcgttgaacc
    16681 acttcaccgt accttttgtc ttcacaaaac cctcctttcc tcaacggcca cgcactcctg
    16741 cgtgagccgg gcccgaaccc agttaacgca cgctacatga ataatgaatg aacaatggaa
    16801 tatgagaacc ggggacggat gggcgggctc tgtgcagaag aaaagacgta ctcacgagac
    16861 ccatcttggt tacaaatcgc gcgcgcagca tgtaccatgc gcattctaat cttgtcaagc
    16921 cgctctcagc accgaagggg tcctgcccgc ccgtgtggac aagcccgtac cagtccccta
    16981 tagtgtccga catcgggagc gagccgccgc tcatgacgct tcgtacgctg ctagaccgct
    17041 atattttcac cgagttgctc tatcccttca gcgtcagcct cggcatgctg tgcttcatca
    17101 tgctgacccg cgagctgctt cggctggtcg agcttctcgt caccaagggc gtaggattat
    17161 gggccgtcct caacgtgttt gcccacctgc ttccgtcgtt tctcgtgctg accctcccga
    17221 ttgccgggat catcgcgtcc atcaccgcct ttggcagact gtcgctggac aaggagctgg
    17281 tggccatgcg cgccgccggc ttgagcctgt tgcggctggc acgccctgtg atgctgttcg
    17341 ccctgctggt gtgcggtatg acgctctggc tcgcgcaatg ggggcagccc tggagttcga
    17401 tcaatctgaa aaaggtggcg ctgagtttgc tgcgggatca actcgtgttg gcgctggaac
    17461 gcggatcgtt caccgaaccg attccgggca tgacgattta cgtctcggac aaaggcgaac
    17521 agccttccgg cggcatcttt atttcggacg gacgaattgc ctcagagcct cgcgtgattg
    17581 tcgcccagga ctaccgcgtg ctgatcgaca gcgccagcga ccaagtggcc ttacggctgc
    17641 aggacggcgt gattcatacc aagccggatg aaatcgatga gtaccaactg gcctcctttt
    17701 ccagctatga cctcaagctt tcccttaacc aaagcggata tgcatcgacg gaggaacggc
    17761 cgtcgtacga tcaattggta gcccggttga acgcctcaca gtggcgcgac ccatgggccc
    17821 tccggcgcct caccgagtac tataaggaca tggcctttcc aacggcttcg ctcatcctgt
    17881 gcatgctcgg tcttccggtc ggaatcgtct cgaaacggtc gggccgtatc ggcgggttcg
    17941 ccgtaggggt gctcgtgatc atcgcctact acgtgttgaa tgtggcttgt gagtttctgg
    18001 tcacagcgac ggccatccct ccgttcgccg gcgcctggct cccgaacagc gtcttcttgg
    18061 tcattacact cgtgagcttc tatcgcatga gcaagcaata gccgcgtatg cccattctct
    18121 ttcgatacct gctgcgcgaa tacgcgaaga ttttcctgat gtgttttgcg ggcctgatga
    18181 ccatttatct ggtcatcgat ttctttgaaa aagtccgccg cttcttgagc ttcgatgcga
    18241 cgtggctgga tgtgctgagt tactttgcac tcaaaattcc cggtgtgtcg taccaaatcg
    18301 ttccactcgc cgtcctgatg gccacgctgc tgacgctcgg cttgctctcg cgcaacaatg
    18361 agattaccgc catccgcagc tgcggaatca gcctcccctg gattgcctca cccttcctga
    18421 cgttcgccgt cctcatgtcc ttggtgctgt tgctgttcag ctcgacggtg atcccgctcg
    18481 cgacgaacaa ggccgaagaa attcgtgtga tccgcattga aaagaaaccg ccctctgcgg
    18541 tgctgacgct ctcgcagcca tggacacgag tcgacgagga cagcctgctg cacgtcagca
    18601 gtcttgctcc cggtggagcc acgttaggcg gcattcagct cttccatttt gatccagcgt
    18661 ttcgactcat cgatatgacg gaagcgcagg aagctcatta tgccgaccac acctggacgt
    18721 tacaccgcgg gtttcagcgc cgcttcaatc gcgacggctc cgtgaccctc atggaattcg
    18781 accggaaacc gattcaactt gcgatgattc cctcggactt cagcagcgga cttgccggag
    18841 attctgaaat gatgacgttt caggacatcc gtgaatcccg cggacgcctg tatcagaacg
    18901 gatcgcaatt tgcccggttg ctgaccgact actacgggcg tatcgcgttt ccgttggtca
    18961 cgatcatcat ggtcttggtc ggcatcgcgc tcagtcttcg ccgaagcggc acccgcggtg
    19021 gcagcatggc gatcgggatc ggacaagcct tgctggtcgg cttctcctat tgggctgctc
    19081 actcaattgc cattgccttc ggacgtgggg gcgcgcttcc accggtaatc gccggctggt
    19141 tgacgaacgt cttgtttacc agctatggcc tgtatctcat gttgaaactc aagtattgag
    19201 cgacgcggaa ctcacggttg ccgaggcgtc ggctcaacgc gactcggttg acgcacgtcg
    19261 tcgcttccgg taagtaaaga cacaacgcac aagagggatg acagaatgcc gggacaggtc
    19321 gttgtgaccg gattgggtat cgtcagcccg atcgggatcg gggtgagcga attttggaaa
    19381 gccgcactcg cgggtcattc cggcgtctcc atgatcacga gtttcgagcc cttttctatg
    19441 gaggggtacc gctcaaaggt tgccggccaa gtgcgcggct tttcccccga acggtatctc
    19501 gacagcgtgc aagccgaacg agtggatcgg tacgcacagt ttgcgctggt cgccgcgaag
    19561 gaagccctgg cggactccgg ccttcagatg gctcgcgagg atccgcgtcg ggtcggcgtc
    19621 attgtgggcg ccggcatggg tggcatggtc atgggagaac gggagatcac ccaactctac
    19681 caacaccagc gtcctcaccg cgtccatccc aattttattc cggtgatcac ccttaactcg
    19741 gcgtcgggca tcgtggccat ggcgcatgga gccaaggggc ccaattacac cgtgtcaacc
    19801 gcctgctcat cgagcgccca tgcgctgggg caggccatgc aatgcatcaa ggaaggccgt
    19861 gcagacgtcg tcattaccgt cggcgctgat gccagcatca cgccgctagt cttcgcagga
    19921 ttttgctccc ttcgagccct ttccaccaag tacaacgatc agccggaacg cgcctcgcgt
    19981 ccctttgacc ggcttcgaga cggattcgtg atgggtgaag gcgccggcgc actcgtcttg
    20041 gaatcctccg ttcatgccaa aaaacgccgc gcgagaatct acgccgaggt ggctgggtat
    20101 gcggccacca gcgaggccta tcacatggtc attccccgcg aagatgggga agagatcgcc
    20161 acgaccatgc gcttggccat ggccgacgca gggctcacac ccgcgaacat tgattacatc
    20221 aacgcgcacg ccacgtcgac cacgatcgga gacgcggttg agacaaaggc tcttcggcaa
    20281 gtctttcgca cacgcgcgga caaactcgcc atcaatgcca cgaagtccct ggtcggccac
    20341 accttgggag ccgctggcgc catcggcggc atcgcaaccg ttctggccat tcacacgggg
    20401 cagatccatc caaccgccaa ctacgaggag ccggatcccg cctgcgcatt gcgaggcctt
    20461 agctcatcgg tgcaggaacg gaaggtcaag gcggcgctgc tcaacgcatt cggcttcggc
    20521 agcaataatg ccgccgtcgt catcaagcgt tatgccgcct gagagcacgc ccgccgtaaa
    20581 ggacagagcc ggatgagcga ccgcgcagtc accgagaaaa tcatccaagc cctcgccgac
    20641 tatttgcgac ggaatccgga gtccatcgat cccaaacatc atttgcggga cgacctgggt
    20701 ctcgactcca tggccgtcat cgagttgctc tataagattg aagaggcctt cgacatccaa
    20761 ataccggacg aagacttggt gcgcctgtcg accgtcggag ccgtctgcca gtacgtagag
    20821 caccgtctgg caccggccaa gcggccggcg gctcgcagcc gtgcagccaa gagtaggaaa
    20881 aagaactagt tcccatggga tccttgtcta cgcctacgac gctgacgctg tcccagattc
    20941 atgacctggt cggcggcacc ctccatggaa acggcgcctc tgtgctgtca tcgctggcca
    21001 gtctggatga cgccaccccg caagcgttgg ctttcatctc caacgacaaa gccgccaagg
    21061 tcccgggcgg gattaaggcc ggcggattac tcgtgcaccg ccacttatca gaaatcgatg
    21121 ttccgcaaat cgtcgttccc aatccactcc tcgcctttgc gcaggtggcc caaacgtttt
    21181 ttatccggcc ttccgctccg cgaggcattg cagcagacgt ctcgaaggga accggcgtca
    21241 cgatcgggac cgacccctcc atctggccct tcgtcacgtt gggagaccgc gtgaccatcg
    21301 gcgcgagggt gacgctgtat cccggggtct tcgtcggggc cgattgcacg gtgggcgacg
    21361 acagcgtgct ctatcccaat gtggtcgtcc gggaaggctg ttcgatcggc gccagagcca
    21421 tcattcacag tggaacggtc ataggagcgg atggctttgg atatgttcag catgagggcc
    21481 gacaccagaa gattccacag ctcgggggcg tgaccatcga agatgacgtg gaagtcggcg
    21541 ccaacgtcac gatcgaccgc gcgacgctgg gccgtacgat catcaaacag ggcacgaaga
    21601 tcgacaatct cgtccagatt gcacacaacg tcacggtggg cgagcacgac atcatcgtgg
    21661 ctcaagtcgg gattgcaggc agtacgacaa tcggccatca cgtcatgatc ggagggcagg
    21721 ccggactcgc ggatcatttg acgatcggag accaggtgat gattgccgcc cgggcaggtg
    21781 taaaccgcaa tatcgagccg aatcagatcg tgtcaggggc gcccatcatg ccgcatgaga
    21841 cgtggatgaa agcccaggcg gtcattcccc gccttcctga gctgcggcac ctggtcagga
    21901 acctggaaca acggatggag aagatcgaat cccgcatcaa gagcaaggca gcgccgaaag
    21961 gcaaacggtc caaacgcggc cgggcatgag agggatcttc cccctagttg acgattcccc
    22021 gccaaaaaaa cagtttgctc caatagaatc cggcccaggg catcaggatc gccggcactg
    22081 cgtccatcgc cccctgcatg atggcccagc ctgacgcgga caacagcagt ccgcatccca
    22141 acacataggc caccgggata aggctgcggc cccgatggac gatgggagga atggtggagc
    22201 cggctttggc ctttcggtgt gatcgctgga ctgcgctgcg cattcgagcg gcgaacacat
    22261 cgggaaccgt ctccaagaga tatcgatggt agcgtgtctg caaccacccg cagagtccgc
    22321 cgagcaacag aagcgcggag ctcgtcatga acgtcatcat tgtataagtg gccgatgcaa
    22381 tgagctgata accaagcgtc ccgattcccc ccagcacgca catcagtcca aggaaccctg
    22441 aagggagaag aatataggtc cggacgtaat gtcgggcttg ggcatgaatg tcctcattgc
    22501 gccagccctt caccagtttt gacatcgtga ctcgccgtag agaccgcttg gagttgcatc
    22561 atagcattct tcggagagtg aagcagacat cgccgacgct cacgtagtcc acaaagggat
    22621 ctgcagtgcg gatacgccac aactcctgcg caggccatct cacgtcagtt caatggagat
    22681 gcgtcaggat aaaaaccgtt tggttcggtg aaggatttgg gagcagcaga tgaaagacaa
    22741 gaacgctaac cgctgaacgg gatgaagcgc ggactagccg actggtaaga tctctgcaat
    22801 gaccgctctt tccacgcgcc cgatactcac ccattgctca gccatgggca ccaacgcctg
    22861 gattcttgac tcaaccgctg caagccgttt cgccagcgtc ggagttttcc ggtatgccgc
    22921 caccatcaga aatgctcgaa ggccttgcag gaacatcgtg atcatgccgg cttgtacggt
    22981 ttctgacgca tcatggaggg cttcaacctg gtcgaggatg ggtgtgagct gcgaagacgt
    23041 gacaaaatcg ctgtcatctt tctgcaactc ggccagaatg cgggtcacga caggaatccg
    23101 tcggccgact tcgtccacga atcgttcatc catccgatcc agctcttcca aaatgcgacg
    23161 gatgccgtcg accgtcacct ccccgtcttc ctgttccgcc cgacggatga ccccatccag
    23221 cacatcacga gtcggctgga ccgatcggga tcgaacatgc tgaaggtcac gcaatgcgct
    23281 caataatgat gcgccagtgg tcattcgcag gggggcgacg gaagaggaag tcttggtcac
    23341 ttccgttcca ctgaattcgg gacgctcatc ggccggcggc cttccttcgg cctgacgaac
    23401 cgcagcggcg atccgatcga ggccagactt gagcgaggcg aacccggcag ccatggcccg
    23461 gggttcgtgg cgtcccacat cctgaaggac gggaaggagc cccaaggcca ggtcctcgat
    23521 gccggtcaaa tgcacggtgg ctgcagatcg ggccagatta ctcaatccct gcagcataat
    23581 gccggagagc ttagatctgg tcgttccgct cgaaccttcg ctgagccgct tcaaggccga
    23641 gtgtatctgg gccagccact catgtgcctc gagcgcgaaa agaccgatca cttctttctc
    23701 aaagctggca tccaagacat cctgctcggt tgaacgaccg gtagacccat tgtcggcccg
    23761 cgtctcggtc ggccccgtgt cggatgccga cgaggtgcgt gactcgacta cgccctgaat
    23821 ggcgctggcc agcgcatcca gattctcgag caaggacgta aattgatcgg agaccgtggg
    23881 aagcgtttta gaccgtcggt cagccagcgc gtcggagcga aacgacaccc gtccaacgtc
    23941 gggggactcg accgtttcga tgatggatac cagcggcgac agactgagat cgatctcgcg
    24001 tttatccgct ccgtttccgg gagtggaatc ggccacaacc tcgacgatgg gacgcagggg
    24061 aaactgcaca ttgattccca cgaccgatcc cacttcaccc gtcgtgagct gcaccagcgt
    24121 gccgagcgga taggccgaaa gctgttcgac cagcgccttg atgatttcac gcggaaaggc
    24181 cgaccgctcg acgaccagta actcgcgcac ggcctcatgc ggaaaaaacc ggcgacgata
    24241 cggacggggc gtgacgagag catcgaacac atcgacggcg ccgatgatct gagcaaactc
    24301 atggatgtcg cgggctttta accggttcgg atagccccgt ccattggagc gttcatgagc
    24361 ctgccgaacg accagagcga gccactgcca ttctccgccg agctttttga ccaactgatg
    24421 gcccaattcc gggtgctgct cgatcagcgt gcgttcctcg ggggtcagct tccccgactt
    24481 cgtcaccacg gattgcggga cggcaaacaa cccgatgtca tggaccagtc cggccaacgt
    24541 gaggcgttgc agttcttccc catagtatcc gaggccggat cccaccttcg tcgccaggat
    24601 gctcacgttg atcagattgg tgatcaacgt ggagccgctc ggtcccgcca acgcctgcac
    24661 gaccagttga tcgttgcggg cgatcgactc tgcaatctcc cgagccagtg cagacaactc
    24721 cttgaggtca agcggatctt ggcgttgaat cgccgaggcc acggacgtca gcgtgtgctc
    24781 tgtccggcgg tacagatctg ttgctcgtcg ttcttccatc tcaatgcggt ggattgaggc
    24841 cccgcccacc tggttcatga cggatcaatt caggatgcaa tcggccgtgg ggcgtgtcaa
    24901 gacaagcaga cgacgacggt catctcgctg gcagcgcctg gcctatcgcc gcccgttcgc
    24961 ttctgcctcg ttcgacccac tgatgaatca gcttccggac gacgcccagt ctgtcggtaa
    25021 tggcctgcac ccgcggtgcc gccaggttca tgtgatgttg tgcgaggacc gataagaggc
    25081 tgtgcaaccc actgaaaaag gtcatggccg gagtgacgtt gacctgttgg gcttcggctc
    25141 tcaattgggt gacttctcga atggatgcct caagcacgcc gccgggcagg cttgctggat
    25201 cggcaagtga agacaattga gccgtcaagg tcggcagctg ctcgtccacg acctggacaa
    25261 acgcctcttc cgactccgac atccggtcga gatagccttg aatgacatcg acgccgacgt
    25321 gctgtacacc agcctgtacc tgtccttcca tctgatcgat catggtctta ataagatttc
    25381 gctccaatgg tcccgtcgtc tcctgtgtcg actggagctt ccgcaacgtc tcaacgaact
    25441 cggtcggcga caatcctgga gccgcctcga ccgtttcgct ttcagcctcc gcgtcgaagg
    25501 acacgccggt ggcgttggtc aatgccgtgt ggatctgccc gagctgtttg cacagtgaga
    25561 gaaaatcctg cacggaaaac gatttggttg gatctttgat ggcctcaata aaaggcagcg
    25621 cggcaaaact cgcctgttcg acattcggaa gattgatcgt ggcagcggat ccgccgagat
    25681 tggtcacgcc ggcggtcagc gtattgatca agacggcgtg acgctcaggc gggggcccct
    25741 gctgtatctc gtcgagggcg acgtgaatgt tctgcaacca ttcatgcgcc tcttgaacga
    25801 acaattcgac gagttcattt tgaaattcct ctgatgtatc gcccgacatg gatgcactcc
    25861 ctcacctata cagcgaccca gccggagcag ccggaacatc ggggagtcac gggcgtcacc
    25921 tgcggatgtt cgaggaaaaa tcggacggct tgtcgcattc gtacctcgac ctgctgggta
    25981 aagacggcag acgcctcacg cgcgcttgga tccactcccc agctgggaag ccaagcccgc
    26041 gatctcgccc aacttcttgg ccatttcttc aaagcgtctg gtcgcctgct gagcggccat
    26101 ttccgtctct gagacgcggc gggccaacac tgcattgcgc tctcgctcgg tctccaagag
    26161 ctgctgaact tctttcgctt tggtccctcc cgtttcctga tcggctagcc ggcgggtgag
    26221 ttcttcgacg cgctgctgtt cgcgtgcgaa ttgctgagcg agctcttggg aacgattcac
    26281 ggccttctcc atggtcgtca tctgctggac aagctgcgtc gcggcttgac gctcggcatt
    26341 caacgcggcc tcgagctctt gaacccggcc gacggatttt tgcatttcag cgatgcgctg
    26401 cgtctgctgg gcggccagcg cgcgctcttt ctggagcgcc ccttccagct ccgagactcg
    26461 ggcggcctga ctttgcagat cggcaacttg tttcttgaat tgtgcactct gttcacgttc
    26521 atgcttcaac aagggttcga gctctttcaa acgttgcagg tttgccgtca attccgtaac
    26581 ggctttcttc gcctgggcgg tctgctcacg ctcggcgacg agcatggctt cgagctcccg
    26641 catgcggccc gactgcacct ccagttcctt catgcgtgcc gcttggacct cgagctcttt
    26701 caggcggcca gactgcgctt ccagctcttt gatccgtcct gtctgggtct cgagttgcga
    26761 gatccgctgc gtcagcagcg acgtctgccg gcgctcgttt tgaagctcct cttcaatttg
    26821 cttcagcttc tcaatcgcct ggcgcgccgc aggcccctgc tcgtcaagaa cttgagcgtt
    26881 ggctggtgcg gtgggcgctg cggatgtggc ggcggctttg gccggagccg gcgcaggtgc
    26941 gctctgcttc ttgccgagaa gttcgaggac ccggtctttg agggaggtgc cctggaacgg
    27001 cttcttgagc acgccatcgg cgtgacagga ctccgcctgc ttcgtcactt cgtcgttgac
    27061 gatgccggag atcagcagga ccggtgtatt ggccagggcg gcctgcccgc gaacgaacgc
    27121 acagacctca tacccgcttt tgtcgggcat gatgacgtcg gatacgatga gatcaggcac
    27181 ttcccgcgcg agcaacgcca gggcttcttc accgttggca gccaatgtca cgcctagccc
    27241 ggcctccgtc agcagccgtt cggcgacctt gcgaacggcg atgctatcat ctgcgaccag
    27301 gatcttcggc atgtatcagt cctttcgctg ttcaacttac gaccctacgt agtcctggcg
    27361 attgcggccc agcctttgta gcgccacgat ctcgatatcc tgtccttctt cgctaaaact
    27421 tccgagcgtt tcgagatcca ggcgcttgct gggaaggatc tgagcgacat ccacattgtg
    27481 tagcgatggg atcacgccgt cgatacaaat cgccatcggc ccgtcgacgt ggcgggccac
    27541 cagacacagt agcgtatcgc cttccgggtg ccggctaagc cggcctccaa gatcataaac
    27601 gggcatcacg tctttatccc gtttcagcat ggctcccaca aacggcgtac ggctggggac
    27661 cggcaagggc tcgatccacg gcgcgatacc gccgacatcc tcagttctag ccgccagctt
    27721 tttcccgccg acggaaaaca cgaccacgtt ccaccatcgt ttggcgctaa cctggtcatg
    27781 tttttgcact gctcgtaaca tccgtcaact atgaggcgga gagggctcgt tgtccggtgg
    27841 ctcccgccac aacttccccg agttcaccca gcacccattc cggagtcagg atcaacacga
    27901 gctcgtcctt gcacatcgtc gtccctgata tccaggtgcg ttcttcgtgt tggaagtgcg
    27961 agggcaacgg ccggcactcc tcccgctcga cttcgatcat gccaatgaca gcctcgacac
    28021 gcatcgcccc attcgtctga ccgttggaat agaggacgac gcgcatgtcg ggattggccg
    28081 gatcgagcgt gaccccgagc cggcttgcca gatctatatc ctgataggtg atgccgacag
    28141 ccgtgacctg gcttccggta ccggcctcag ccggcgtgag gacgccccgg acgccttgcg
    28201 acggcagggc gcagagtatc ctgtcgaagc gcacgaccag aaatctggcc gtctgcacca
    28261 cggccgtggt cgcgatatga tggccacgca aactcatgac tgtgtcactg tcctccctcc
    28321 atccttgcgg ccaggccgcc gggaggtcct aggctttccg tgcgggcgac tcttgtccga
    28381 tccagcgttc cagctcctgg atcagcgccc gctcttcaac cggcttggcg atatacgaac
    28441 tcgcgccgac attcagcgcc atctggcggt gtttgtcacc ggcacgcgtc gtcatgacga
    28501 taatcggcgt ctgttgcgtc tgcgatctgc tgcgtagcgc ctgaatcact tcatatccgt
    28561 tcagcttcgg catctcgaga tcggtcagga tcagccggta attcttcgcg gacgccttgc
    28621 gaagtccttc ctcgccgtcc acggcggtat cgacctcata gcctgcggtt tccagcatgc
    28681 gccccacgaa ctttcgaatg cttaacgagt cgtcgatcag gagaatcttt tgctcgacgg
    28741 tcggctgagg catcggttct tggaacggcg tctccgtgtc ggtgcctgcg gtcgtctcat
    28801 tcagtgtttc caactgcgag ccggcttgct cgccacccga gaccagccgt gcaggatcga
    28861 gcacgagcac cacgcgccct tcgggatcga tggtggcccc gccgaaacag gaccgctcca
    28921 atgatttaag cgccccgatc ggcttaatga cgatttcctg ccggccgagc aactcgtcca
    28981 cggccaagcc gacaggcccc gtgccggtgc gcacaatgac gaccggtttt cccacttcca
    29041 cccgggccga gccgttccga tacatcagct gctgcagagg ctgcacgtcc acggcttcgt
    29101 ctccgagatg cagcatcgac cgctcgccca tctgttggag ggaaccggcc gtgagcatcg
    29161 tcacctcacg gacgctcggc agcggaatcc cgtaacgttc ctctccggaa cggaccatca
    29221 gagcagtcgc gatgagcagc gtcagcggca aatggagcgt aaatttggta ccgacaccgc
    29281 ggatggattc gacgtcgata tggccattca tgctctcgat gacccgtttg acgacgtcca
    29341 tgccgacgcc gcggcccgcc tgatcaccca cctgctcggc ggtcgagaag ccgggaagaa
    29401 agatgaactt gatggcctcg gcttcgggaa ggtttttggc gatgtcagaa cgcacaaggc
    29461 cgcgctcgac cgccttcgcg cggattttgt cgatatcgag accggcccca tcgtcttcca
    29521 cttcgatcag aacggcattg cctcgatgag aggcgtggag atagatcgtg ccggccgcgg
    29581 gctttccctt ggagacgcgc aacgccgccg gctcgatccc atggtagacg gcattgcgaa
    29641 ccagatggac caacggatcg acgagacgct cgacgacgcc ggtgtccact tccgtgtgtt
    29701 ctccggaggt gaccagggcg acttccttac ccgttgccct tgccatttcc cgagtcgccc
    29761 ggcggaagcg tgtgaacggc gtgccgatcg gcaccatacg cgcgcgggcg atctcatccc
    29821 gcatgccgag tgtgagttgc tgcagttggc tcatgtcctc atgcgaccgc cggatggatc
    29881 cgctcaactg cgtcatggac tcggagatgt ccgccgtgac ttcgctgatg cgccgggcca
    29941 gaatgttgaa gtcgtcgtac ttgtcgaatt cgaggctgcc gaaatcgctc agtccctgga
    30001 aggccgccgc ggcactaccc gtccttgcct gatccgctgt cccgccggcg gaggtgggaa
    30061 gtgtaaaggt gtgtttctct tcgaacgagc gaacagaatc tgtcaatcgc actttgcaag
    30121 ccaagacttg ctgcgacagc tgctccaaga cactgagacg ctgctcgagc cgcccacgac
    30181 cgatgaccaa ttcgccgacg aggttgagca gccgttccag ccgatcacgg ctgacgcgaa
    30241 tgacttcccg ttcctcaacg gccgcatccc tcaccttgcc ctgctcaggc tcgggttgct
    30301 gagcagcctg ttcgacgacc ggctcgggca tcggctgaac aggagccgct ttgacaacgg
    30361 tctcgaccgt tttcgtctgt tcaagttgtt tgagcgcggt catcgccgcg gcaaaccgct
    30421 gacgaacggt tcccgtcagc gtgggatcac ggcgcatcaa taggcgcacg acatccaccg
    30481 atcgcaggag gacgtcggta tgccccggca ggaccttgat ccggctttcc cgcacggcac
    30541 ccataaaatc ttcgatgtaa tgcgtcaggt cgccgatcga ttggaagccg acggtatagg
    30601 cagacccttt cagcgtatgg gcggtgcgga acaattgatt gatcaaatcc ggattctgcg
    30661 gttctttttc aaggcgaagc agttgcgctt ccaaggactc caaatactcc tgtgcttcgg
    30721 gcgcgaaata ggacaagacc tccgggtcga gctgcggcat gagatactca tcggtcaatg
    30781 agggagcagg caccgcagcg gcgggctgat ccgtgacaac cgacggctct aacacagccg
    30841 tcgcaacctg ggcgacgggt cgttcctgtg catgcgcgat ggccggcgcc ggtgcgccga
    30901 cgggctgacc gctctggatc tgttgcaagg tctgggtgac acccgggacg ccctggcgca
    30961 attgtgcgag atggctttcg tcccggcgca tcaccaggcg aatgagagcc gcagcatctc
    31021 cgatcgtcgc caggagcgct gtcgacacgg ggatgtgtcc gtcgcgcacc gcgatcatgc
    31081 aggattcaat cggatgagca atgtcgccga tgaccttgaa cccgatggtg tgtgcagacc
    31141 ctttgagcgt atgggcggtc cggtacagcg cctggatggt ctctgcgtcc ccgggatttt
    31201 ctttgagacg gttagtcagg acctccaatg tctggagata ttcatcggct tcaggggcga
    31261 aataggacag cacttcggca tcgatggtcg gaaccaggta gtccgtgccg agctctttcg
    31321 aaccatcctc ggaggacgca tctgaggaag ccggcaacag ggtcatgacc tctttcttcc
    31381 aatgggcact caccgatggg tcctcctggc cgccctgctt gatgacatcc agttgcgatt
    31441 gaaacgcttc gaccatgccg cgcaacgcac cgaccgtctt gagccacaga gccgggtcga
    31501 tggacgtaga ctgttcgagt gtcgattcga ggagctcgcc caacagcgag aggcccgtat
    31561 acccgtagat gccggaagcc cctcgaactt tgtgcgccca tacatactga tcctggagct
    31621 ctgaggcgct cggcacgctg tcgccgggag gattcacggc cgccgagagc gcagcgagcg
    31681 cttctgcggc ttccattata aagatgtcga ggaggccctg acggtccagt tcggagctca
    31741 tccctatcct ttctgacccg gagcgccggt acggcgctcc gggtaccaga ccttaggcca
    31801 gcttgaactg cgcgaccgac gccgtcagac cgtcggcgag tttggccatg tcttcgacgg
    31861 tcaatcgtgc cgcatcggtg gctttctgcg tcgccacggc accgccggtg aagtccttga
    31921 tcgagcgacc cacctggtcc gtagacgccg tctgtgtcgc agcggaactg gcgatggtct
    31981 gggccaattc ggcggaccgt tgggcgatgc ctgaaatctc gttgaacact tcaccggtac
    32041 gcagcgcgga agccgatccc gcttccaccg cctgggtttc ttgttccatg gcgacgaccg
    32101 cgtcctgtgt ttccgactga atcactttca ccagatccgc gatttcgcgg gtcgcttgcg
    32161 tggagctttc ggccagtttc cggacctggt cggcgacgac cgcgaaccgt gctccggctt
    32221 ctccggcgcc tgccgcctcg atggcggcgt tgagcgccag caggttggtc tgattcgcaa
    32281 tgtctcgaat cgtcgacacg atctgtgaaa tttccagcga ccggtcgccg agtcccttca
    32341 cctgcttcga catgcgttgc accgctgaac gaatgctctg catgtcctgc acggtttcct
    32401 gcaccgcgac acgcccgcgc tccgtggctt gcaacacttg tttggcggat tccgaagagg
    32461 cgcctgccgt ttcggctacc tgacgcattt gcgaagcgag ctgctcgacc gcgctgagcg
    32521 tcttagccga ctcgtccgct tggtgtttgg cggttccggc catctgaccg gcgctgtcac
    32581 ggagcgtgcc ggctgacttg ttgacacgtt cggccgcctc acggacctgt ttgagcagct
    32641 gtccgaaccg ggcgatcatg aggttgaatc cgtccgccag gttgccgaac atgtcggccg
    32701 tcacttcacc gcgcttcgtg aggtctcctt taccgacatc cgacacgaga acaaggaagt
    32761 tcatcaaacg cttttgcatc tcgtcgcgtt cgccttcgga cgtcaccagc gccgtaatac
    32821 ggtccaacat ggtgttgaac gaggtggcca tctggcccaa ttcgtccttg gacgcgatct
    32881 tcgcgcgggc ttggtaattg ccgctcgccg cttgctgcgc gacgctggcg acctgcccga
    32941 tacccagtga gagcttgtgt gccacccaat atccgagggc caaacccaac agcacggccg
    33001 cgataccgcc gaccaccagg atcatggtgg cgttggcagc catgcctttg gcttcttcgg
    33061 agaggtcttt cgccacttcc gacatggtct tgatctgttc ggcatggcgt tgcgtcacct
    33121 tttcataggc agggctcata ctcaccgtga tggccagggc gcccaattcc cgcatggcca
    33181 gagcctggga tgatgtcagc cccttgttct cgaaactttc cttgatggtt gtgattgcgg
    33241 catcgccctc attgaagaac tgggtgaccg cggccttcag ttcggccagg tctttggctt
    33301 catctcggcc ggtgctggat gttcgtaacg tcgtcttttc atagctcgcg aggagccgtt
    33361 cgacttctgc ccggtacgga gcaatgtcct tcaactcgat ttcgaagtcg gaggcgcgcg
    33421 gcgtgcggga gacgtcgacc aacgcttcgt ggtaccggtt gacgttgaac agcagctggt
    33481 tgaattcggt caacgggatc gtgtaatcga tgtacacggt ttccgcttgc gtcgccaagc
    33541 ggctgttggt cacgataccg acgccggcca ttgcggcgat gatgacgccg accaagccga
    33601 acaccgtcag cagtttggcc tgtgtcttta agttttcaaa ccatttcaga aagttgagcc
    33661 tcaacatggg aacctcctcc ttctcctagc gatccgatcg caccatgagc atcacaccct
    33721 gtcctgatga tcttccgact atcctgtgcc ttcgaaatga gaaagtatgg ccgatgtctc
    33781 caagaccccg cgaagccggt cttccagctt cacgaccgtc gacacgaagg gattgactcc
    33841 gtctcccgtg ccgaccggag ccggtaaaaa ctgatccttc gacaaggtgc gaatttcggg
    33901 aaccgtatcc accaagaccc cgacctgcca gttgccgtgc ttgacgacca cggcatactt
    33961 gagagcggtc tcagcggcca cgccgatcat ctgcctgaga tcgattaagg ggacgacgga
    34021 tcctcgcagg ttcgtgacac cgatcaatcc ggacggcatg cccggaaccg gcgtcaccga
    34081 ttcgaccaca aacacttccc gtacgttttg caggtcgatc gagaacatct cgccccctaa
    34141 cgagacgatg gccgcccgta tggtcgatgt gccggaagtt ggggcagcac tcccttgcga
    34201 ggcaacgatg gcatcggcgt gggaggtcgc gaccgaggtg gcgccctgcg gagcactcat
    34261 ggcggttatc ccaacacccg tttcacggcc gccagtaatt cgtcaccctt gaacggcttg
    34321 acgacgtggg cattggcacc ctgctgctga ccccagaatt tgtcactttc attccctttg
    34381 gaggtgcaga ggatgatggg gatattcttc gtccggtcgt cgttcttgag atcgcggcag
    34441 gcttgaaaac cgttacggcc cggcatgacg acgtccagga cgatcaaatc cggcttctca
    34501 ccaaccacct tgtcttcgag cttctccgtg ttggggaggg acaccacggt atgattcgcc
    34561 cctttcaaca ctccctcaat cacctggagc tcggcgtacg aatcgtcaac gacaagaatc
    34621 ttgctcatcg gttctctcct tgttctggtt atgaatgacg tccgatttcc actacttcag
    34681 aggcagtacg acgctgatcg caccggccaa atcgccctct ttccagccct ctttatgacc
    34741 acccgtaata tctttgccgc ccttgggcgc gccgtggcat gacaaacaag acgccgccgc
    34801 gtactcaggg tccatcatgc gcagcaccgg ccggccgtcc agcatcgtat tgcggacgta
    34861 cgtttggccc ttcggatggc gcggatcact gaacatccga agcacttcgg cctcgaaatc
    34921 gtccggtctg ttgctgacgt tgcggtaatc gattcccgtc agttttaact tcacccctgt
    34981 ctttttataa aagttttctc cggtcttccg ggcgaatacc gcagggatga agcctttgaa
    35041 gccgatgccg gcacggttga tcaccggttg agcttcatcg acgacatctt tttccgcctc
    35101 gaccagcgcg agatacagac ctgcttgggg aacggcgttg ggaattctga ggtcgatttt
    35161 ggtcttcttt ttgaaccgct cgatgacctg agcggccatg tagtcgccgg tgaagccctt
    35221 gttgcttttg gaggcgtcgt tgatattccc catctgctcc gacatgactc cacggccgat
    35281 cttgaccaga tcgatgagat gttccgccac ttcggcctcg ccggccgcca ggaccgtgct
    35341 ggcattgaac atcagcgccg ccgccgcgat tccgcccgcc atccgccaat tgaaccatgt
    35401 cgccttcata cctatcctcc ttcgtgaagt cactcgtccg gatgcttcgt ttcttttctc
    35461 attcttcgcg cgcagcacct agcaattcct gtttttctac aagatgtcgc cgatgcaccc
    35521 gcgcatgacg ccgcggcgaa ccgctcgaag cttcggctca ctgtgcaaag gttgtgctta
    35581 ttcgcctaca aacagcgccg acgtaaatac ggaatacaaa cgcctgacgg atcagcggtt
    35641 tacgtactct acacatgagc atcattcaga ctcgtccgcc gcacgactgt tcaggttgcg
    35701 acaaaatgat gccgtttgtg caaaaattga ccactcgcct ccatccgtgg cgccaggatc
    35761 agcaccggaa agtcgagcga agcgtaagaa tgggattcca cttagggaaa ataaaaccta
    35821 aggaaggaac tgacgacgtc tttactctct tacagagcct tgttccgtcc agcgacgcga
    35881 cggagccctc gttccacaca acggccgacg agccgcccga tcatcgcgcc catctcggta
    35941 tgcgtcccgc tgtactgcaa cttatggtca cctgccaaac tgctcgcaag caccaccgca
    36001 tcggtaccgg tccctgtcgc cagggcggct ttttcatagg cacttgggac cttgtgtttg
    36061 atgagtgttg cggtcttgct ttctgtggcg acttgcacgg ctccgatcaa agcaggaatc
    36121 gtcaaggttg cgttcgtcac caggataata ttgatcgtgc ctgcctgatc gcggtccagc
    36181 gttctttgca gcctggaacg aaccggttcg cctgcccgaa cggcgttggt gaccccgacg
    36241 gtacaaaaac attccagcag tagggagcct gattgttcac ggtcgacgac aagctgtgtc
    36301 atgggcacag cggtcatgag gccgacaacc ggcatggcgg ccctcacgct ccgtgccagt
    36361 cgccccaaat atctcgccgg atccgaccat tcctcgaccg accgaccgcc gggcatgaca
    36421 gggttggccg ggacttgatg attgaggatc gatcgccccc gaacgatccc ccctccgcgc
    36481 ggcgccgaag agacgatccg atacctcatc gcaagatcaa tgaccaaggt attcccacag
    36541 actcgatact tgacgccctc cctcttggtg tgttgtcggc gcatgctatc cctcgagtac
    36601 ctgggccaag gcggccagca gtctatcgtt ctctcccttg gtccgcaccg cgatgcgtat
    36661 gcttcccagg ccacagccct ccactgacga gcagtcccgt atcagcatgc ctcgtcgtcg
    36721 aagagctgcc gtcatcaggc cggcgccgca tgagggcggc agctcggcaa aaagaaaatt
    36781 cgtggaggat ggaatgaccg tcacacctgc cagcgcggcc agctgcttcg tcatgcgcgc
    36841 gcgttctcgg ccgacatacg cgagacaacg tcgcgcatgg cgacggtcag cgatggccgc
    36901 ttccgcagcc cgttgagcca ccgtgttgac ggaccaaggc ggctggcgat ctttcaggat
    36961 ggccgcgacg gatcgcgaag ataggctgta gccgattctt aaccccggca tcccataaaa
    37021 tttggtaaag cttctcaaga taatcaaccg tggatacgcc tgcagcctcg gcacacaggt
    37081 caatgcgtca cagtaatcga taaaagactc atccaacacc acccatatcc gcccacgctc
    37141 agccgcttgg aacagatcat ccaattgttg aagatcacca ggcctgccag tcgggctgtt
    37201 gggatgacaa atgaagacgg cgtcaatcgc gcggcggccg ctccgttgca cctgcacacg
    37261 atgcgtgacc cgctcaagcg ggggtctgta ctcgtcgttc cgtctggcca acaccatctg
    37321 atatcgaccg cccgcacgag caacggctcg cgcatattct ccatacgttg gaccgacgat
    37381 caacgctgaa cggataccca gggcgcgcgg aatgacatca atgagctcac tcgagccgtt
    37441 tccgatgacg aggcggtcag gcgaaatctt ccaacggcgc gcaatgctct gtttcacgct
    37501 gacacaatca ggatcagggt agtgctggat cagcggtgcc gcggtagcca gcgctcgtat
    37561 gacttgcggc gagggtccca aaggattaat gctcgcactg aagtcgagga gggatccgat
    37621 cggtcgtcgc agttcccgag ctgcggcgta gacattgcct ccatggacga gcgcgttctt
    37681 cagtgccata agaatcccag gcacgcggcg atggcgatca gataggcgac ggtcatgatg
    37741 tgctgtgctc gagtaatgtg agcggcgagc aagggttctt gggggtcccc aagcagcggc
    37801 cggtcctgtc gcagtcctgc ataaaaattt gttcctccaa gctgaacgcc gagggcaccg
    37861 gccatggccg cttccggcct tccgctgttc ggactgggat gcttgtgccc gtcccgcagc
    37921 aaaagcgccc cgcttcgatg gaccgatcgt gtgttccagc acacggcccc ggccgcgagg
    37981 gcgatcaacc agccggtaag acgcgcaggg atccaattga cgacatcatc gagcttcgcg
    38041 gagccccacc caaaatcctc ataggctgca tctcgatgac cgatcatcga atccagggtt
    38101 gagacggcct tgtaggccaa ggccaagggc ggccccccta cgacaagaaa aaagacaggt
    38161 gcaatgatgc cgtccgaggc gctctcggca atggtctcca ctgtcgccct cacaatctcc
    38221 ggttcagata gacggtcggt gtcacggccg acgatcaagc taaccgagtg acgtgcgcca
    38281 tccaacgtgc cggtttggag ggcgtgggac acggcgccca catgatcgac gagatcgcgc
    38341 caggccagca cagtggatgc caaccaaatt ccaataccac gcccgatcca gtcatgaatc
    38401 gccgtaccgg tcgcaatggc gacccaaccg gcgcaaaatg cgaggctcgg caggcccaag
    38461 gccagcgcaa aaccggtgac gcgaagcgag agtgatccga cacagaatcg gcgcgtccac
    38521 ttctccaccc gagtgatcag tctgcccatc catcggaccg gatgaggcca cgcaggtgga
    38581 tcaccgatca cagcatcgac gatgcacgct gccaacaagt caagaggagt cacggtaaat
    38641 acgtgaggaa tggcatggac aggaggaagc tgatttcaat gacctcgttt gttgccccga
    38701 gggtgtcgcc ggtgattccg ccaaagtaag atcgacagca cagcgtggcc agccgactca
    38761 ctatgccgcc gatcagcagg attgtcagtg cagccgcgaa gccaaaagac cacaccatca
    38821 gggaggcgac aagcaccgtg gcgccgagca cgtgccacgc tgagagatgt gctaaaaatg
    38881 gggcggccaa gcctccttcg gctcgcgcat atgaagcccc gtaggcgccg acaaccatgg
    38941 cccagcggcc gattgccggc gcacagagca gagctggaaa tttcagcggt tcaggcagtg
    39001 agacaagcgc gctatagcgt aggattaatg agacaaacaa accggtcgcg ccgatggcgc
    39061 cgatgcgagg gtcacgcatg atggccaggc gatcggtcgg cacccgccct ccagccaatc
    39121 cgtccagcgt gtcagccagg ccgtcctgat gcaagcctcg ggtcatcgcg acgagcaaca
    39181 agatgaggag cgccgcaacc acctcacgcg acagccatcg ggccaagagc atatccgtca
    39241 tgaccatgaa cgcgccaagg aggattccga cacagggata ccacaacata gatcccgcca
    39301 gctcgtgggg cgccggatca tgaaggtccc ggctcagagg aaccgccgtc agaaactgcc
    39361 aggcaaaaat gaacggcctg agactggtcg tcactggttg cgctccgaga cacctgcttc
    39421 gctgaacgtc gccatctccg tgagtacttt gacggctgcc tgaacgagac tcattccaag
    39481 acaggctccg gtgccttctc cgagtcgaag atccagatcg aagagaggct ctaaacccag
    39541 tgtctctagc accagccgat gccctctctc gacggaccgg tgagaagcga tcatatattg
    39601 gacgcttcgg ggctggaggc caccagcgat caatgcggct gctccggcaa tgaacccgtc
    39661 caagacgacc ggtatgcgcg cggcggccgc accgagcatg agaccggcca tgcccgcaat
    39721 ttccaagccg ccaatttttg tcaggacatc gatggggtcg tccgcattgg gctgatgacg
    39781 ggccagcgct cgctcaatca cggagacttt tcgtgtccgg ccaagttcgt cgatcccggt
    39841 tcccaggccc gtgacctgct caaccgacgc cccagtgatg accgctgtga tcgccgcgct
    39901 cgccgtcgtg tttccgatgc ccatctcacc ggtaccgatc agcgtcacac cttctttcac
    39961 cgcctcggac gcgagttcaa tcccgatctc cagggcctct tcgaccgttc gccgatccat
    40021 ggcctccccc tgaaccatat ttcgtgtgcc catgagcaat ttccggttga tcaggccggc
    40081 aacggagc
//
LOCUS       DASSCQ010000007        19186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1012645, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000007 DASSCQ010000000
VERSION     DASSCQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 19186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012645"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFH05_00380"
     CDS             <1..724
                     /locus_tag="VFH05_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676701.1"
                     /translation="QWNILGRPGYGEGDLVYSEKPQMAVGGGLAYNPGINTSTDNAFV
                     GIDLANLNFRRQLATFGNGRQLGWGVINYTTYGFDSVFKYRGFSLQGEWYFKNMERTF
                     KGAPCLQFNAAGACTAVTPQLLGNATGWYVQSGYYLIPRKLEVAARYSYWDPDTNASG
                     DLIKQVDASINWFPFGTYDYQLMLTYSNMAMGTGGYAIGRSAPLPTQPPGTVNPSGLV
                     PLDAVGGTLVENAIRVQLQIFF"
     gene            849..1652
                     /gene="modA"
                     /locus_tag="VFH05_00385"
     CDS             849..1652
                     /gene="modA"
                     /locus_tag="VFH05_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6676702.1"
                     /translation="MNGLKSYIRQVFLAVSVTLITCVGGHPALYQEALAEEITIAAAS
                     DLNFAIKDLIAEYEKATGNHVKLSLGSSGNFFAQIQNGAPFDLYFSADIGYPKKLDEA
                     GLIMPGSLYRYAVGRIVLWTNHASHRDVSKGLDLLRDPAIKKIAIANPKHAPYGRAAV
                     AAMQHFKVYDDAKDRLVLGENISQAAQFIESGACDVGIIALSLALAPTMKAAGSYWEI
                     PPNAHPPLEQGAAILKSSKQQMTAQQFVAFMQSTQGQEIMSRYGFTLPK"
     gene            1630..1947
                     /locus_tag="VFH05_00390"
     CDS             1630..1947
                     /locus_tag="VFH05_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676703.1"
                     /translation="MDLRFRNRPAWLLTLLLWSGCSDAVQMTHETEQGGVVTFLFKED
                     RGGPMGSPHRKDALKLIDRKCPAGYVVVRDGELKGYSSMSSVEGQEGDVTNRRWGIQF
                     RCK"
     gene            2092..2295
                     /locus_tag="VFH05_00395"
     CDS             2092..2295
                     /locus_tag="VFH05_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET6676704.1"
                     /translation="MKLSARNQFQGTVTRITEGQAMAEVSVKVGHLEFIAAITEGSVK
                     NMGLKVNDAVTVAVKATEVMIGK"
     gene            2439..3113
                     /gene="modB"
                     /locus_tag="VFH05_00400"
     CDS             2439..3113
                     /gene="modB"
                     /locus_tag="VFH05_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HET6676705.1"
                     /translation="MNWTAIWVTCQLASLTSLALLVIGLPIAYWLTFSKWRWKFLIES
                     IVALPLVLPPTVLGFYILVAIGPHSPLGRLYSDVVGQSLPFSFQGLLVASILYSLPFA
                     VQPFATAFEQVDRKLIEASWTLGLSKLKTFFKLIVPLSTAGLITGAVLSFAHTLGEFG
                     VVLMVGGNIEGVTRTVSIDIYDDVQALNYAAAAKTALFLLAVSYVVLLFIYSMNRKVW
                     AVWPQK"
     gene            3098..4189
                     /locus_tag="VFH05_00405"
     CDS             3098..4189
                     /locus_tag="VFH05_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6676706.1"
                     /translation="MAAEIAIDITKTFPGRPAISAKLTYTLQQSTALVLFGPSGSGKT
                     TILRCLAGLEWPERGTIRFVSRTWLDTGSRIRVSPQARLIGYMAQDYALFPTYTVAGN
                     IAYGLDALSTLERKKRVAEMLELFQLTGLEEAKPDELSGGQQQRVALARAIAPRPQLL
                     LLDEPLSALDVPTRVQLRGELRSLLKHLALPSVIVTHDWAETLTLGDVVAVVGDGHVL
                     QVGPPQEVFSRPVNAAVAQIVGVETVIEGEVIEQANGLATVRVNGTILKGLGTNQIGS
                     PVFVCIRAEDVLLESAGSGMTSARNHLQGKVSEIIPHGVMVHVKVDCGFTVVAAVTKG
                     AQEELGLEQGSPVVAVVKAGAVHLVPRSM"
     gene            4615..5160
                     /gene="lexA"
                     /locus_tag="VFH05_00410"
     CDS             4615..5160
                     /gene="lexA"
                     /locus_tag="VFH05_00410"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET6676707.1"
                     /translation="MKPADLRRIREQLGLTQQQLAEALRTTRVSVARYEAGMRRIPGM
                     VKVAIDRLGRAPEIPMAGMVAAGFPIEPVQQSELIDVPPSMLQGGKTFALRVKGESMK
                     DDGILPGDLIVVRKQETAQSGQTVVALVNGEATVKTYFKKATHIELRPANTTMPPILV
                     QASDEFHIEGVLIGVIRHCAT"
     gene            5394..5576
                     /locus_tag="VFH05_00415"
     CDS             5394..5576
                     /locus_tag="VFH05_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676708.1"
                     /translation="MLVQEPDIDIKRSGPCADCGIRVERRIECVDGLTHSTVELCQAC
                     WIACQQRQAFAGGCCG"
     gene            5588..6328
                     /locus_tag="VFH05_00420"
     CDS             5588..6328
                     /locus_tag="VFH05_00420"
                     /inference="COORDINATES: protein motif:HMM:NF020014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676709.1"
                     /translation="MQAVMQSAEATQLPLVEEEPQQTLLGRRGEPGSEWFLALRHAQI
                     PFHSDDRLILLYGHPLVFRLGLHAATERVLAGEPVVYLDGANTFDPFVVGQLARAHRR
                     QPRALLSMIHVARAYTGHQMERLISDCLMFALERYQSRIAIVSGLFNTHYDQAVPEHD
                     EMRVFGRIMEASQRLTQQGHILFFLCPQLSVLTHTAQRSFDRLRLQADRIIRVGEERG
                     LVKLQEEGEAVGKSWEIAGTALERRPGR"
     gene            complement(6425..6601)
                     /locus_tag="VFH05_00425"
     CDS             complement(6425..6601)
                     /locus_tag="VFH05_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676710.1"
                     /translation="MSDRLRDDFVVDMDERRFENASVKRITEIEALWRINAQRNTLLP
                     REPDAGAGIWKLAA"
     gene            6820..7488
                     /locus_tag="VFH05_00430"
     CDS             6820..7488
                     /locus_tag="VFH05_00430"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HET6676711.1"
                     /translation="MQTASRPFLFKECSELREILGEEAYDEKRLVELLETVPLDSIYF
                     HTHSYFLRHSYVERVYPNDFAQWVAMEVRDHVLGERLAVVDPFEYGELDALRGQLISI
                     IDDHLSKTPIVPRVMFGEPFYFNKSLIIQVPTRIEVRTLQEFRNALSEVEVSAIYFHV
                     FEARHRLKHEESDFSAWIHDGLGMPDLAEKLRAINPYLGSLERVRSALLTVCDEFLNR
                     EHKP"
     gene            7509..9182
                     /gene="treS"
                     /locus_tag="VFH05_00435"
     CDS             7509..9182
                     /gene="treS"
                     /locus_tag="VFH05_00435"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET6676712.1"
                     /translation="MSDPSWFKDAVFYELHVKAFHDGNDDGIGDFRGLLEKLDYIQWL
                     GVDCLWLLPFFPSPLRDDGYDVANYRAISPEYGSLEDFRHFLDAAHQRGIRVIVDLVL
                     NHTSDQHPWFQQARSSPESATRDYYVWSQTDKRYEKARIIFIDTEKSNWTWDPAANAY
                     YWHRFFSHQPDLNYDNPEVQREMLDIMSFWLDEGLDGFRCDAVPYLFEREGTICENLP
                     ETHAYLKEIRKRLDQTYGDRILLAEANQWPADVRPYFGNGDEFHMAFHFPLMPRLFMG
                     VRSEDWHPIVDMFTHTPSIPENCQWCLFLRNHDELTLEMCAGEERDYMYYAYARDPQM
                     RRNIGIARRLAPLLDNDRRKIELLNSLVFTLPGSPIIYYGDEIGMGDNVHLGDRNGVR
                     TPMQWNADRNGGFSKADPLKLYLPPISDSVYGFHVTNVDSQRQSPHSLLHTMKQMIAR
                     RKTQPAFGRGTLTFLHPKNDKVLAYIRRYRGNELLLVHNLAGSAQPVELDLSAFQGQV
                     PIELLGGSRFPRISKGLYSLSLAPYGFYWFSLEQPQHDDPSYGIEGSVV"
     gene            9208..10407
                     /locus_tag="VFH05_00440"
     CDS             9208..10407
                     /locus_tag="VFH05_00440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6676713.1"
                     /translation="MAHELDEYRDHAPKGTVEFLYRLSDQVAGHSFLHISAGRYGGGM
                     AEILRRLIPMLNTLGINARWEVLAGDQEFFQASRRLANSLQGQDDYLTDQMQEVYLKI
                     NARTAQSLNLDADLVMVHDPPPAALIEHRKGGKWFWRCYLDIAKPRQHSWSFLRRFLV
                     GYDAAIFSLPGFAHRLPIPQFLISPSIDPLSEKNRPMPRVEVNQVLSRLGIPRDKPIL
                     LQLARFEWFKDPLGAIRAYRLVRKHHNCRLVLAGSGVMHDPEGEAVLAQVHEAAAGDP
                     DVHILQLPPEADVEINALQRAATIILQKSFKEGFSLAVTEAMWKGKPVIGSTAGGLAA
                     QILDGVTGYTVHSVEGAAFRARHLLNNPGLLQRMGGAGREHVRRNFLVTRHLSDYLTL
                     MRLFVTR"
     gene            10450..11730
                     /locus_tag="VFH05_00445"
     CDS             10450..11730
                     /locus_tag="VFH05_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET6676714.1"
                     /translation="MGEAVWEQVQEQCSDVRTVDLLVALPSFNQSSTVGPVVHAIVAG
                     LKSAFGQASILFLNADVGSQDGTPDLVRQTVGDSLPFASIRPNTDGMSGSTFALNRFS
                     YSGMPAREEAFRSVFAVTRRLEARACIILDPSVRSITAEWVALLAQPILEKGADYVAP
                     VFSRQRYEGSLTNNLIAPLTRALYGKRVACQIGGAYGFSQKLSSFLLENEALWEGDAG
                     RFAIDSWMTTVAVAEGYEVWQACLGSKVQDTKAGAADISTSLAQAVGATFHYMERYQD
                     IWEKQVESQEVPIVGRPVEINMESVPIQVDRMLKGFRQGLRDLLPIWEVILTPDTLAG
                     VLELGLRDDEEFRFPLPLWVQTVYDSAVAYHEKVIHREHLLKSLVPLYLGRTASVVLE
                     TKGGGPGMVQDTVEHGCLTFEVMKPYLIDRWRFQ"
     gene            11727..12425
                     /locus_tag="VFH05_00450"
     CDS             11727..12425
                     /locus_tag="VFH05_00450"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676715.1"
                     /translation="MSEMWQDAMVAAFREMASRGVLFLPKLMALVTFLMLGLAIGWLV
                     QVSLMRILNVVRFEVFCERMGLTPALTKAGMKQQIPHLVGRLAFWIVFLFFAFMGVDA
                     LDLPATANLMSAILGFLPNVIAAILVALLGILLANFTGEAVLIAAVNAQIHGARFIAN
                     MVRWGVVIFTVAMVLTQLGIAKEIVVAAFSIIFGGIVLAMAIALGLGGRSIAKEVLER
                     RFGREKSRDEFTHI"
     gene            12452..14446
                     /locus_tag="VFH05_00455"
     CDS             12452..14446
                     /locus_tag="VFH05_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET6676716.1"
                     /translation="MTHQDGRQRVVIEGVRPEIDGGRFPIKRTVGEEVVVEADAFCDG
                     HDVIAVVLRYRLRDSTEWTDLPMKHLGNDRWRAAFHVSTMGQWHYTIAGWVDHFETWR
                     RDLVKKLDAGQNLQVDLLIGATLIESAVRRASADDGPKLTAWRQALRSNDSPDDDRIR
                     IALSADLRALMARYPDRRLASIYERELQVIVDRQKARYSAWYELFPRSWSRIPGQHGT
                     FKDCEAALPYVASMGFDVLYLPPIHPIGQTHRKGKNNAPTGGASDPGSPWAIGALEGG
                     HKSIHPELGTLRDFRRLREQASTYDIEIAMDVAFQCSPDHPYVKEHREWFKIRPDGTV
                     QYAENPPKKYQDIFPLEFETDQWRELWDELKSVILYWSSEGIRIFRIDNPHTKPFPFW
                     EWVIRETKQIYPDVIFLAEAFARPKVMLRLAKLGFTQSYNYFPWRTTKSELTEYFTEL
                     TQTDIREYFRPNLWPNTPDILTEQLQHGGRPAFMSRFVLAATLGASYGIYGPAFELGE
                     RVPREVGSEEYLHSEKYEIKQWDLRREDSLSEFIGHVNRIRRENPALHSDWNLHFHEV
                     GNDHLLCYSKRTSDGSNVILVVVNLSPFHVHSGWLELDLAYLGVEPDRPFQVQDLLTN
                     AHYLWQGRRNYIEINPHSAPAHIFRVKGKVHSERDFDYYF"
     gene            14476..16098
                     /locus_tag="VFH05_00460"
     CDS             14476..16098
                     /locus_tag="VFH05_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HET6676717.1"
                     /translation="MLTAEQDWARLLEGDAAADLMRVLPDILQTRRWFGGKARRIAEV
                     RIVDSIAVPSDSSSILLLLCVEYQDAETETYSLPVTAVFGEDAERVQRDIPRAVIAPF
                     MLTKNGTTDLGILYDALWNREFTVELLTALRKESRFQGAVGTVKASVTAAFTDLVPSE
                     IRGDPRVMGSEQSNTSVVYDGHVILKLYRRLEQGINPDLEIGRVLTQMQFPFVPALAG
                     AIEYQRPSQEVSTLGVLQRYVENEGDAWTWSLQAVKEFFARVGSWPLDRHAARDSFPL
                     LELTGEPYPEPVSQLMGVYPEAAERLGRRTAELHLALSRVPDDPAFAPEPLTGDYRQA
                     RYEAALGGMASALALVKDRMDSLPTAVQEQAILLFGFRPALERIFGAIRNLNTRGSLI
                     RCHGDYHLGQVLFTGADFVITDFEGEPARPLAERRMKRPPLVDIAGMVRSFHYVPFAL
                     CKGAGLPPSPWCPFWSNWACVAFLKGYLSTATGSELWPQTNGDVGLLLDAYVAEKALY
                     ELRYELNNRPDWVDIPLHGLEAILKRREESER"
     gene            16173..18065
                     /gene="glgB"
                     /locus_tag="VFH05_00465"
     CDS             16173..18065
                     /gene="glgB"
                     /locus_tag="VFH05_00465"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET6676718.1"
                     /translation="MTLLTKDDLYLFNEGTHFHLYEKLGAHPLESEGTRGTHFAVWAP
                     NAEHVSVIGTFNNWQRGAHVLSPVKASGIWQGFLPGVDNGALYKFHIRARGGNYEVDK
                     TDPFAFFNEIPPKTASIVWDLSYTWHDQSWMDRRSSQNSLNAPISIYEMHLGSWKRVP
                     EEGHRSLSYRELANSLPGYLEQTGFTHVEFLPIMDHPFFGSWGYQTTGYFAPTGNYGT
                     PQDFMYLVDELHRHGIGVILDWVPSHFPTDEHGLSYFDGTHLFEHEDPRQGFHPDWNT
                     YIFNYGRNEVRSFLISSALFWLDKYHIDGLRVDAVASMLYLDYSRQEGQWIPNQYGGR
                     ENVEAISFLRRFNEEVYRNYPQVQTIAEESTAWPMVSKPTYLGGLGFGLKWDMGWMHD
                     TLSYMSQDSFFRKYHHNKLTFRMLYAFQENFVLPLSHDEVVYGKGSLLGKMPGDPWQK
                     FANLRLLYGYMYAQPAKKLLFMGDEFGQWSEWAHDSSLEWHLLEQERHRALHTWVTDL
                     NRLYRTERALHERDTDPTGFEWIDCNDAEASVISLLRKGASPDDLLFVVCNFTPVPRT
                     NYRIGVPRGGFWQEVLNSDATLYGGSGWGNLGGIEAVPVPLHGRSHSLTVTLPPLATL
                     FFRLAR"
     gene            complement(18062..>19186)
                     /locus_tag="VFH05_00470"
     CDS             complement(18062..>19186)
                     /locus_tag="VFH05_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6676719.1"
                     /translation="LKEQPDLNWQNAAVRAAMWDVVKFWLDRGIDGFRLDAINWLGKD
                     MRWPDNPFRFGLRGYTRQHHIHDRDRELAHQIMRELREVVSAYRDVVLIGEAAADTPG
                     GPASFYGTGDDELHLVFDFRVMKSTWSAETFRRFLSEHVTALPKGAWPTIVLSNHDQS
                     RHIDRYGHRGDAKARARLAALLLLTMPGTPFLYYGEELGMRNGRLRYRDLQDPYTKRF
                     WPFRTGRDPARTPMQWDGSRHAGFSTERPWLPVSPDYPQLNVAQSTSNPQSLWSLYRQ
                     LLHHRRTSPALTRGDCRLVPGGPGECLLYERVASLENGAHERILVAVNFSERTVAITM
                     PDGSSSGQVLLSTDTQKRGPVWDPRRVQLGPQEGVVVRLF"
BASE COUNT         4117 a         5112 c         5640 g         4317 t
ORIGIN      
        1 ccagtggaac attctcggac gaccgggata tggtgaagga gacttagtct attctgaaaa
       61 accgcaaatg gctgtcggtg ggggattggc ctataacccc ggcatcaata ccagcaccga
      121 caatgcgttc gtcggcatcg atctggcgaa tctgaatttc cggcggcaat tggcaacgtt
      181 cggcaatgga cgtcaattgg ggtggggagt catcaactac acgacgtacg ggttcgatag
      241 cgttttcaag tacaggggct tttcacttca gggggaatgg tacttcaaga acatggagcg
      301 aaccttcaaa ggtgcgccgt gtttgcaatt caatgcggcc ggcgcgtgta ccgccgtgac
      361 gcctcagctt ttgggcaacg cgacgggctg gtatgtccag tcgggatatt atctgattcc
      421 acgcaaactc gaggtggccg cgcgatactc ctattgggat cccgacacca atgcatcagg
      481 cgatttgatc aaacaggtgg atgcgtcgat caattggttt cccttcggga cctacgatta
      541 ccagctcatg ttgacgtaca gcaacatggc gatgggcacg ggcggatatg cgatcggccg
      601 cagtgcgccg ctgccgacgc aacctcccgg taccgtcaac ccctcaggct tggttccgct
      661 ggacgccgtg ggtggaacgt tggtcgagaa tgccatccgc gtgcaactgc aaatcttttt
      721 ctgagccagg gagccattgg aacgtgtata cagtcaaagt gcatggaaga atcgaccatc
      781 gagaccctgg gacctgtccg aggagcgcaa gagtcgcgga ggccggcata cataggaagg
      841 atgcatcgat gaacggctta aaatcataca ttcgtcaagt attcctcgca gtttccgtga
      901 cgctgatcac atgcgtgggc ggtcatcctg ctctctatca ggaagccctg gcggaagaaa
      961 taacgatcgc cgcggcctcg gatttgaatt tcgccatcaa agacctgatt gccgagtatg
     1021 agaaggcgac cggcaaccat gtgaaattat ccttgggatc gtccggcaac tttttcgcgc
     1081 aaattcaaaa tggggcgcct ttcgacctgt atttctcggc ggacataggg tatcccaaga
     1141 aattggacga ggccggcctg atcatgccag gctccttgta tcgctatgcc gttggccgga
     1201 tcgttctctg gaccaaccac gcttcgcacc gggatgtgtc caaggggctg gaccttttac
     1261 gggatcctgc gatcaaaaag atcgccatcg ccaatcccaa gcatgccccc tacggacgcg
     1321 ccgcggtggc ggcgatgcag catttcaaag tatatgacga cgccaaggac cgattggtct
     1381 taggagaaaa tatctcgcaa gcggcccagt tcatcgaatc cggcgcctgc gatgttggca
     1441 tcattgcact ctcgcttgcc ttggcaccca ccatgaaggc cgcaggcagc tattgggaaa
     1501 tcccacctaa tgcccatccc cctttggagc agggagctgc gatcttgaaa tcatcaaaac
     1561 agcagatgac cgcccaacaa tttgtggcgt tcatgcaaag cacgcaggga caggagatta
     1621 tgagccgcta tggatttacg cttccgaaat agaccggcct ggctgttgac gctcttactt
     1681 tggagcggct gctccgatgc ggtgcaaatg acgcatgaaa cggagcaggg tggtgtggtc
     1741 accttcctct tcaaggaaga ccggggtggt ccgatgggat ccccgcatcg aaaagacgca
     1801 ttgaagctca tcgaccggaa atgcccggcc ggatacgtgg tggtgcggga tggagagcta
     1861 aaaggttaca gcagcatgtc gagcgttgag ggacaggaag gagacgttac gaaccgccgg
     1921 tgggggattc agtttcgatg caaatgatcc acctaatccg ctggtctatt tcggtagtca
     1981 cggccaggtg tcctgctaaa gaccgcaggg ccggaaggat tcatgatccg ttcgatcaag
     2041 tgaagtgagt catgtcagga tgcaacaata aaacagcttt aggaggacag catgaagctc
     2101 agcgcacgca atcagttcca gggaaccgtc acccgcatta cagaaggcca agcgatggcg
     2161 gaagtgtcgg tgaaggtcgg acatcttgaa ttcattgcgg cgatcactga aggctcggta
     2221 aaaaacatgg gcctgaaggt gaacgatgcc gtgacggtgg cggtgaaagc gactgaagtg
     2281 atgatcggta aatagcggat gtcaggatgg tcaaaacggt cgtcagcgaa gccacagcag
     2341 gcgcagaggt gagacgtact ctcgctcgga cgttgagctt ctcagcgatg cgaggacaaa
     2401 gctgacggtc tttttcatca tcctggaaag gcggcattgt gaactggacc gctatctggg
     2461 tgacctgcca actggcgagc ctcacctcgc tcgcattgct cgtgatcggg ttaccgatcg
     2521 cctattggtt gacgttttcc aagtggcgat ggaaatttct tatcgaatcg atcgtggcgc
     2581 ttcccttggt gcttccgccg acagttctcg ggttctacat ccttgtggcc attggaccac
     2641 atagtcccct cggccggttg tacagcgacg tcgtgggtca aagcttaccg ttcagttttc
     2701 aaggcttgtt ggtcgcctca attttataca gcctgccttt cgccgtccaa ccgtttgcca
     2761 ctgcctttga gcaggttgac cggaaactca tcgaggcgtc atggaccttg ggcctttcga
     2821 agcttaaaac ctttttcaag ttgatcgtgc cgctttcgac ggcggggctc atcacagggg
     2881 cagtcttgag ctttgcccac accctcggcg agttcggggt tgtcttgatg gtcggaggaa
     2941 atatcgaagg cgtcacccgg acggtttcca tcgacattta cgacgacgtg caggctctca
     3001 actacgccgc cgcggcaaag acggcgctgt ttctcctcgc cgtgtcctat gttgtcttgt
     3061 tgttcattta ttcgatgaat cgaaaagtct gggcagtatg gccgcagaaa tagccatcga
     3121 catcaccaag acgtttcccg gccgtcctgc catcagcgca aagctgacat atacactgca
     3181 acagagcacg gcactggtgc tcttcggtcc ttcgggctct ggcaagacca ccatcctacg
     3241 ttgtctagca ggattagaat ggcctgaacg gggaaccatc cgatttgtgt cgagaacctg
     3301 gcttgacacc ggttccagga tccgggtttc gccacaggcg cggttgatcg ggtatatggc
     3361 gcaggattat gccttgtttc cgacctatac cgtcgctggc aatatcgcgt atgggttgga
     3421 tgcgctgtcg accctcgaac gaaaaaagcg cgtagccgag atgcttgagc tcttccaact
     3481 cactggtctt gaagaggcca agccagacga attgtccggg ggacaacagc aacgcgtcgc
     3541 gctagccaga gcgattgcac ctcggccgca gttgcttctt ttggatgagc cgctttccgc
     3601 cctcgatgtt cccacgcggg tgcaacttag aggagagctg cggagtctgc ttaaacactt
     3661 ggccttgccc tctgtgattg tcactcatga ctgggctgag acgttgacgc tgggggatgt
     3721 ggtagccgtg gtcggggatg gacatgtcct acaggtcggt cctcctcaag aggtgttttc
     3781 gcgaccggtc aatgcagcag tcgcgcaaat tgtgggagtt gaaaccgtca ttgaaggcga
     3841 ggtcatcgag caagccaatg gcctggccac cgtgcgcgtc aacggcacaa tcctgaaagg
     3901 gttgggcacg aatcaaatcg gttcgcctgt gtttgtctgc attcgggcgg aagatgtgct
     3961 cctagaatcg gccggaagtg ggatgaccag cgcgcgcaac catttgcagg ggaaggtgag
     4021 cgaaatcatt ccacatggcg tcatggtcca cgtgaaggtc gactgtggat tcacggtcgt
     4081 cgcggcggtg acgaaaggag ctcaggaaga gttaggactc gaacagggtt cacccgttgt
     4141 cgccgtggtc aaagcgggcg ccgtgcatct cgtgccgcgc tccatgtaac gattattccc
     4201 aaacccaccg gacgtattgc tgacggccac tacccggccc cttccgacct tttgtgattt
     4261 gacctactag gggtctcccc aggttctgtg gataaccctg tgtgtaatgc agtcctttct
     4321 tttcatttca tgcttggaaa gggcacaggc acgctttgcc tatattttga gcataggtgg
     4381 gttagacgca cgcgcaccat atgtggtggt tctatctcaa gaaaacgaac attctgattt
     4441 ccgcatcgaa tgcggatttt ggctgctcga aatgcgaaag gcaggaccta gacgacgctt
     4501 tgtaaaaact tatgcacaga ccccgcattg acagggcgtt ttctcatctt tttttgcaat
     4561 cgcgatcaga gggcttgaca gctaggcagt gggtgtgtaa ccattggtta cagtatgaag
     4621 ccagcagatc tcagacgcat tcgtgagcaa ctcggcctga cccagcagca actcgccgag
     4681 gcgctacgca ccacccgcgt ctccgtcgcc cgttatgaag ccgggatgcg tcgaatcccg
     4741 ggtatggtga aagtggcgat cgatcgtcta gggcgtgcgc ctgaaattcc gatggccggc
     4801 atggtagcgg ccggcttccc gattgaaccg gtgcaacaat cggaactcat cgacgtcccg
     4861 ccaagtatgt tgcagggtgg aaaaaccttt gcactacgcg tcaaaggaga atcgatgaag
     4921 gatgacggca ttctgccggg tgacctcatt gttgtgcgga agcaagagac ggcgcaatca
     4981 ggacaaaccg tcgtggcgct tgtcaacggc gaggccactg tgaagacgta tttcaagaag
     5041 gccacgcata ttgaactgcg ccctgcaaac acaaccatgc cgccgatcct cgtccaggcg
     5101 tccgacgagt ttcacatcga aggcgtactt attggggtta tccgtcattg tgcgacgtaa
     5161 caaggaggca ggattgaaga tgacaatcac gaaagaccga ttcataactc tgcagcggac
     5221 cgtcgataca ttggaaggaa gactccagga catccagtgc gaactacggc atgccaaagg
     5281 cgtctcggtg accgaacgag tgtggaaaca tttactggcg ccctggcagg ggaacgcgtt
     5341 catgtcttcc tgttgtaatc ggcgagtgcg acccaacaca aaggtggtga attatgctgg
     5401 tacaagaacc ggacattgac atcaaacgga gcggcccctg tgccgactgc gggatcaggg
     5461 tggagcggcg tatcgaatgt gtcgatgggt tgacgcattc cactgttgaa ctgtgccagg
     5521 cctgttggat cgcttgccag caacggcaag cgtttgccgg cgggtgttgc ggatagcgag
     5581 gtgcatcatg caagccgtca tgcagtcagc agaagcaacc caactgcccc ttgttgaaga
     5641 agagcctcag cagacactcc ttggcagaag aggggagccc ggcagtgaat ggtttctcgc
     5701 attgcgccat gcgcagattc cctttcattc ggatgatcgg ctgatcctct tgtatgggca
     5761 tccgctcgtg ttccgcttgg gacttcatgc cgcaaccgag cgggtcttgg cgggtgaacc
     5821 ggtcgtctat ctcgacgggg cgaatacatt cgatccgttt gttgttggac aactcgcccg
     5881 cgcgcatcgg cggcagcctc gtgccctctt aagcatgatc catgtggcca gggcgtatac
     5941 cggacatcaa atggagcggc tgatttcaga ctgtctgatg tttgccctcg aacgctatca
     6001 gtctcgcatt gcgatagtgt caggcttgtt caacacgcac tatgatcagg ccgtgccgga
     6061 gcacgatgaa atgcgggttt tcggtcgaat catggaggcg agtcagcgac tgactcagca
     6121 gggccacatc ttatttttct tatgtccaca actttctgtt ctgactcata cggctcagcg
     6181 tagtttcgat cgtttacgct tgcaggctga ccggatcatt cgggtcggtg aggagcgtgg
     6241 actcgtgaag ctgcaggaag agggggaggc ggttgggaaa tcatgggaaa ttgccggcac
     6301 agcattggag aggcgtccag gcaggtaagg gtcttcactc ccgtgccagt tatcagggat
     6361 ctgttttacc ccttcgcttg cgagcttggt tccgaacaat gcaggcctga caaaaagagc
     6421 ctcattacgc agccagtttc cagattcccg cccctgcgtc aggttctcgt ggcagtagag
     6481 tgttcctttg cgcattgatg cgccagagcg cttcgatttc cgttattctt ttgaccgacg
     6541 cattttcaaa ccgccgctcg tccatgtcca cgacaaagtc atccctcaaa cgatcgctca
     6601 ttatggtctc cttccaatct aacagttgag taaggggtac tacgctccac gatacccggc
     6661 aactggtgtt tcccctggga gccacctatt agattcgccg taacaccgac cctccgcatt
     6721 cctcctgaac tggggcaatc cctaggtacc tactagcccc catattggga acatggtcta
     6781 attgtcggaa ggcgcaagac ggcgcaggag gtgagtttcg tgcagacggc gagccgccct
     6841 tttttattca aagaatgtag tgagttgaga gaaattctcg gcgaggaagc gtatgacgaa
     6901 aagcggctgg tggagttgct ggaaaccgtc ccgttggatt cgatctactt ccatacccat
     6961 agttattttt tgaggcatag ctatgtggaa cgggtctatc ccaacgattt cgcgcagtgg
     7021 gtcgcgatgg aagtgaggga tcatgtgctg ggcgagcgtc ttgcggtggt cgatccattc
     7081 gaatatggag agctggatgc cctacggggc cagctgattt ccattattga cgaccacttg
     7141 tcgaagaccc cgattgttcc gcgcgtgatg ttcggcgagc ccttttactt caacaagtca
     7201 ctcatcatcc aagtcccgac acgaatagaa gtcaggacgc tccaagaatt tcggaacgca
     7261 ctgagcgaag tcgaagtgag cgcgatctat ttccacgttt tcgaagcgag gcaccgtctc
     7321 aaacatgaag agagcgattt ttccgcttgg atacacgacg gtctcggcat gccagatctt
     7381 gcagagaaac ttcgtgccat caacccgtat ctgggaagcc tcgagcgggt gcgctcagcg
     7441 ctgctgacgg tgtgcgatga gttcttgaac cgggagcaca aaccctgagc acaaagggaa
     7501 gctctacgat gtcggatccc tcatggttca aggacgcagt cttttacgag cttcacgtca
     7561 aagcgtttca cgacggtaat gacgatggga tcggagactt tcgcgggctc ctggagaaac
     7621 tcgactatat ccaatggctc ggcgtcgatt gtctttggct gcttccgttc tttccgtcgc
     7681 ccttgcggga cgatggctat gacgtcgcca attaccgggc catctcgcct gagtatggct
     7741 cgctcgaaga ctttcgtcat tttctcgacg ccgcgcatca acgcggcata cgtgtcattg
     7801 tggatcttgt gctgaatcat acatccgacc aacatccatg gttccagcaa gccagaagtt
     7861 cacccgagtc ggcgacgcgg gactattatg tctggagcca aaccgacaag cgctacgaga
     7921 aggcgcggat catcttcatt gacacggaaa agtcgaattg gacctgggat cccgcggcga
     7981 atgcgtatta ctggcaccgg tttttcagcc accagccgga tttgaattat gacaaccccg
     8041 aagtccagag agaaatgctc gatatcatgt cattctggct ggatgaaggt ctggatggtt
     8101 ttcgctgtga tgccgtgccc tatttgttcg agcgtgaagg cacgatctgt gagaatttgc
     8161 cggagactca cgcgtacttg aaagaaatac gaaagcgcct cgatcagacc tatggcgacc
     8221 gcatcctgct cgccgaagcg aatcaatggc ctgccgacgt gcggccttat ttcggaaacg
     8281 gcgacgaatt tcacatggcc tttcacttcc cgctcatgcc aagactgttc atgggcgtgc
     8341 ggagtgagga ttggcatccc atcgtggaca tgttcacgca tacaccgtcg attccggaga
     8401 actgtcaatg gtgtttattc ctgcgcaacc atgatgaatt gacgcttgaa atgtgtgccg
     8461 gcgaggaacg tgattacatg tactatgcct atgcacggga tccacagatg cggcggaaca
     8521 tcggcatcgc tcggcggttg gccccgttgc tggacaatga tcggcgaaaa attgaattgc
     8581 tcaacagcct ggtcttcacc ctcccgggca gtcccatcat ttactatgga gatgagatcg
     8641 gtatggggga caatgtgcac ctgggcgatc gcaatggtgt tcggactccc atgcagtgga
     8701 acgcggatcg aaacgggggg ttttcaaaag ctgacccgct caagctgtat ttgccgccta
     8761 tctcggattc cgtctacggc ttccatgtga ccaatgtcga ttctcagcgc caatcgcccc
     8821 attcgctctt gcatacgatg aagcaaatga ttgccaggcg gaaaactcag ccggcgttcg
     8881 ggcgcgggac tctgacgttt cttcatccga aaaatgacaa ggtcctcgcc tacattcgca
     8941 ggtatcgcgg caacgagctc ttgctcgtgc acaatttggc cggatctgcg caacctgttg
     9001 aactggactt gtccgcgttc caaggacaag tgcccataga actgctcggg ggatcccgct
     9061 tcccccgaat tagcaaaggt ctgtactcgt tgagcctggc gccctacggg ttctattggt
     9121 tcagcctcga acagcctcag catgacgatc cgtcatacgg cattgaaggc agcgtcgtct
     9181 agcgcggcgc tgtgattgga accagagatg gcacatgaat tagacgagta tcgagaccac
     9241 gcaccgaaag gcacggtcga gtttctctac cgcttgagcg atcaagtggc aggacatagc
     9301 tttttgcata tcagcgcagg ccgctatggc ggcggtatgg ccgaaatcct tcgccggctg
     9361 attcccatgc tcaacacgtt aggcatcaat gcacgctggg aagtgctggc cggtgaccag
     9421 gaattcttcc aggcgtccag gcggctggcg aattcccttc aaggccagga cgactatctc
     9481 accgaccaga tgcaggaggt ctacctcaag atcaacgcgc gtaccgctca aagcctcaat
     9541 ttggacgccg atctggtgat ggttcatgat cctccgccgg cagcattgat cgagcatcgc
     9601 aagggaggga aatggttttg gcgttgttac ctcgatatcg ccaaaccccg gcagcatagc
     9661 tggagttttc tcagacgttt cctggtcggg tacgacgcgg cgatcttttc attaccgggg
     9721 tttgcccatc gcctgcccat tccgcagttt ttgatttctc catcgatcga tccgttgagt
     9781 gagaagaacc ggcccatgcc ccgtgtcgaa gtgaatcaag tgctctcgcg tcttggaatt
     9841 cctcgcgaca aaccgatctt gctgcagttg gcgcgcttcg agtggtttaa ggatccgcta
     9901 ggagcgattc gggcgtaccg gttggtgaga aagcatcaca attgccgcct cgtgttagcg
     9961 gggtctggtg tcatgcacga tccggagggt gaagcggtct tagcgcaagt acacgaagcg
    10021 gctgccggcg atcccgatgt ccatattctg caattgccac cggaagccga cgttgaaatc
    10081 aacgcgctgc agcgggcggc gaccatcatc ttgcagaaat cgtttaagga agggttcagt
    10141 cttgccgtga cggaggccat gtggaaagga aagcccgtga tcgggagtac cgctggcggc
    10201 ctagccgcac aaatcttgga cggcgtgacc ggctacaccg tgcattccgt cgaaggagcg
    10261 gcgttcaggg cgaggcatct cctcaataat ccgggcctcc tgcaacgcat gggcggcgca
    10321 gggcgagagc acgtgagaag gaattttctg gtgacacggc acttaagcga ttacctgacg
    10381 ctgatgaggc tgtttgtcac gcggtgaaca cacagccatt gaatcgcatg gctcaaagga
    10441 cagtccgtga tgggggaagc agtatgggag caggtccagg aacagtgctc cgacgtgcgc
    10501 acggtcgatt tgttggtggc ccttccgtcg ttcaatcagt cctcaaccgt tgggcccgtc
    10561 gtccacgcga ttgtggcagg gctgaaatcc gcctttggac aggcttccat cctcttcttg
    10621 aacgccgatg tcggctctca ggatggaact cccgatcttg tcagacagac ggtcggcgac
    10681 tccttgccct ttgcgtcgat ccgtccgaac accgacggca tgtccggcag taccttcgcc
    10741 ttgaatcgat tttcctactc cggcatgccg gcacgggagg aggccttccg gtctgtgttt
    10801 gccgtcactc ggcggttgga agcccgagcc tgcatcatcc tggacccaag cgtgcgatcg
    10861 attacggctg aatgggttgc tctgctggcc caaccgatat tggagaaagg ggctgactat
    10921 gtggcgcccg tgttcagtcg gcaacgatat gaaggcagcc tgaccaacaa tctcatcgcc
    10981 cccttgactc gtgcgcttta tggaaagcgg gtcgcttgtc agatcggtgg cgcgtatggc
    11041 ttttcgcaaa aactctcgag cttcttgtta gagaacgaag cgctgtggga aggagatgcc
    11101 ggccgcttcg ccattgacag ctggatgacc actgttgccg tcgccgaggg atacgaggtc
    11161 tggcaggcct gtcttggatc caaggttcag gacaccaagg ccggagcggc tgatatctcg
    11221 acgagtctcg cccaagccgt cggtgcgacc ttccattata tggagcggta tcaggatata
    11281 tgggagaagc aggttgaatc tcaagaggtc ccgatcgtcg gtcgcccggt tgagattaat
    11341 atggaatcgg taccaatcca agtggaccgg atgctgaagg gatttcgtca gggattgagg
    11401 gacctgcttc cgatttggga ggtgattctt acgcccgaca cactggctgg cgtgctggag
    11461 ctgggattga gagatgacga ggaatttcgc ttcccccttc cgctgtgggt tcaaaccgtc
    11521 tatgactctg ctgttgcgta tcatgagaag gtcatccatc gtgagcatct cttgaagtct
    11581 ctggtgccgc tctatctggg tcggaccgcg tcggtggtcc ttgaaacgaa aggcggcggg
    11641 ccggggatgg tccaggatac ggtcgaacac ggttgtctca cctttgaggt gatgaagccg
    11701 tatctgatcg accgatggag gttccaatga gtgagatgtg gcaggatgca atggttgcgg
    11761 cctttcggga aatggcgagc cggggcgtgc tcttcttgcc aaaactgatg gccctggtca
    11821 cattcttgat gctcgggctt gccataggat ggctggtcca agtctcgctg atgcgcattc
    11881 tcaacgtcgt acgctttgag gtgttctgcg aacgtatggg actcacgccg gccttgacga
    11941 aggcgggcat gaagcaacaa atccctcacc tcgtcggccg cctggcgttt tggattgtat
    12001 ttctgttctt tgcgttcatg ggggtcgatg cgttggattt gccggcgacc gccaatctga
    12061 tgagtgcgat cctaggattc ctgcctaatg tgatcgcggc gattctggtg gcactgctcg
    12121 gcatcctcct ggccaatttc accggagagg cggtcttaat cgccgcagtc aacgctcaaa
    12181 ttcatggcgc ccggttcatc gccaacatgg ttcgctgggg agtggtcatt tttactgtcg
    12241 ccatggtcct gacacagctt gggattgcga aggaaatcgt ggtggcagca ttctccatca
    12301 tctttggagg catcgttttg gcgatggcca ttgcgttggg gttgggcggg cgcagcatcg
    12361 cgaaggaggt gctcgagcgt cgcttcggtc gcgaaaagag cagagacgag ttcactcaca
    12421 tttaacttgg cggcagatga atccaccggt catgacacat caagacggaa gacagcgggt
    12481 tgtcatcgag ggagtccgac cagagattga cgggggacga ttccccatca agcggacggt
    12541 cggcgaggag gtcgtggtcg aggccgatgc cttttgcgat ggacatgacg tcatcgccgt
    12601 cgtcctccgt tatcgcctgc gggactccac ggaatggacg gatctgccca tgaagcacct
    12661 tggcaacgac cgttggcgtg cggcgtttca cgtcagcacg atgggacaat ggcactacac
    12721 gatcgccggc tgggtcgatc actttgaaac ctggcgccgg gacctggtca aaaaactcga
    12781 cgccggccag aatctacagg tagacctgct gatcggggcc acactcattg agagtgcggt
    12841 caggcgagcc tcggcggacg atgggccgaa gctcacggcg tggaggcagg cgttgaggtc
    12901 gaatgactct cctgacgacg accgcattcg aatcgcgtta agcgcggacc tccgggccct
    12961 catggcgaga tacccggatc ggcgcctggc ttccatctat gagcgagaac ttcaagtgat
    13021 cgtcgaccgg caaaaagcgc ggtacagcgc ctggtatgaa ttgtttcccc gctcctggag
    13081 tcgcatccct gggcagcacg gcacattcaa agactgcgaa gccgcgcttc cgtatgtggc
    13141 gtcgatggga ttcgatgtgt tgtatttgcc tccgattcat ccgatcggcc aaacgcaccg
    13201 gaaggggaaa aacaatgcac cgacgggggg agcttcagat cccggcagtc catgggcaat
    13261 cggcgcgctc gaaggaggcc ataagtccat tcatccggaa cttgggacgc tgcgcgactt
    13321 tcggcgattg agagagcagg caagcaccta tgatatcgaa atcgcgatgg acgtggcctt
    13381 tcaatgctcc cctgatcatc cgtacgtcaa ggagcatcga gagtggttta agattcggcc
    13441 ggatgggacg gtgcaatacg ctgagaatcc tccgaaaaaa tatcaggaca tctttcctct
    13501 cgaatttgaa accgaccagt ggcgggaatt atgggatgaa ctgaagagcg tcatcttgta
    13561 ttggagcagc gagggaatca gaatatttcg catcgacaat ccacatacga agccatttcc
    13621 tttctgggaa tgggtgatcc gcgagacgaa gcagatctat cccgatgtga tctttctcgc
    13681 tgaagcgttc gcgaggccaa aagtgatgtt gcggctcgcg aagcttggtt ttacgcagtc
    13741 ctacaattat ttcccctggc gcaccacgaa gtcggagctc acggagtact tcactgagct
    13801 gacgcagacg gacattcgcg aatactttcg tccgaacctc tggcccaata cgccggatat
    13861 tctgactgag cagctccagc acggcggccg gcccgcattc atgtctcgct tcgtcttggc
    13921 ggcgacgctc ggtgcgagct acggcatcta cggtcccgca tttgaactgg gagagcgcgt
    13981 gccccgggaa gtgggaagcg aggagtatct gcattcggaa aaatatgaga tcaagcagtg
    14041 ggatcttcgc cgagaggata gtctgagcga gtttattggc cacgtgaatc gcatccggcg
    14101 agagaaccca gcccttcaca gcgactggaa tcttcatttc catgaagtgg gaaacgacca
    14161 cttgctgtgt tacagcaaga ggacctctga cgggtcgaac gtcatcctgg tcgtcgtcaa
    14221 cttgagccct ttccacgtgc attcaggatg gctcgagctg gatttggcgt atctcggcgt
    14281 cgagccggat cgtccattcc aggttcagga tctgctgacg aatgcgcatt atctgtggca
    14341 gggacggcgc aactatattg agatcaaccc gcattccgct cccgcccata tttttcgcgt
    14401 caaggggaag gtacacagcg agcgggattt cgactattac ttctaacaga ggccggtctt
    14461 cacgactgtg aaacgatgtt aactgccgag caagattggg cgcgtctctt agaaggagat
    14521 gctgcagcgg acttgatgcg agtcttgccc gatattctgc agacgcggcg ttggtttggt
    14581 gggaaagctc gccggattgc cgaggtccgt atcgtggatt ccattgcggt cccctccgat
    14641 tcgagctcca tccttctgtt gctttgcgtg gagtatcaag atgccgagac cgagacgtac
    14701 agtcttccgg tcacggcggt attcggagag gatgctgagc gtgttcaaag ggacattccg
    14761 cgagccgtca tcgcgccgtt catgctcaca aagaacggca cgaccgacct cggcatcttg
    14821 tacgatgcgc tctggaaccg ggaattcaca gtcgagctgt tgaccgcgct gcgcaaggag
    14881 tctcgattcc aaggcgctgt gggaactgtc aaggcttcag tgacggctgc cttcacggat
    14941 ctcgtgccct cagaaatccg gggggatcct cgtgtcatgg ggtccgagca gagcaatacg
    15001 tcggtggttt acgatggcca tgtcattttg aaactctatc gacgccttga acaggggatc
    15061 aatcctgacc tcgaaatcgg ccgtgtattg acgcagatgc agtttccgtt cgtgccggct
    15121 ctcgccggtg ccattgagta ccagaggccg tcgcaagaag tctcaacgct tggcgtcttg
    15181 caacgatatg tcgagaacga aggcgatgcc tggacgtgga gcctacaggc ggtgaaggaa
    15241 ttcttcgcgc gcgttgggtc ctggcctctg gaccggcacg ctgcgcgtga tagcttcccg
    15301 ctgctcgagc tgacaggcga gccgtatccg gaaccggtgt ctcaactcat gggggtatat
    15361 cccgaggcgg cggagcgctt aggccggcgg acggcggagc ttcatctggc acttagccgg
    15421 gtgccggacg accccgcctt tgcccctgaa ccgctcacgg gggactaccg acaggcccgg
    15481 tacgaggccg ccctgggagg gatggcaagc gcactggcgc tcgtgaagga ccgcatggac
    15541 tcgcttccca cggctgtgca agagcaggcc attcttcttt ttgggttccg tcccgcgctc
    15601 gagcgtatct ttggggccat ccggaatctg aacacgcggg gttcgctgat ccgttgtcac
    15661 ggcgactatc acctggggca agtcttgttt acgggagccg attttgtgat cactgatttt
    15721 gaaggagaac ctgcgagacc gttagccgaa cgccgcatga agcgtccgcc gctcgtggac
    15781 atcgccggca tggtccgatc attccactat gtgccgttcg ccttgtgcaa aggcgcaggc
    15841 cttccgcctt caccgtggtg tccattttgg tcgaattggg cctgtgttgc attcctcaaa
    15901 ggctatctca gcacggcgac cggctccgaa ttatggccgc aaacgaacgg cgacgtgggt
    15961 ctgttgctgg atgcgtatgt cgcggagaag gctctttatg aattgcggta cgagctcaat
    16021 aataggccgg attgggttga cataccgctc catggtctcg aggcgattct caagaggaga
    16081 gaagaaagcg aaaggtgatc acctgcggcc aggcgaggta gggagtcacc atgacgagaa
    16141 ttcagtttac acccgggagg ccgcgtaagg agatgacctt gctgaccaaa gatgatttat
    16201 acctattcaa tgaaggcacg cactttcact tgtatgagaa attaggagcg catccgttgg
    16261 agtctgaggg gacgagagga acgcattttg ccgtatgggc acccaatgca gagcacgtct
    16321 cagtcattgg gacctttaac aattggcaac gaggcgccca tgtcctttca cccgtgaagg
    16381 cctccggtat ttggcagggc tttctgccgg gggtggacaa tggcgccctc tataagttcc
    16441 acatccgagc acgtggcggg aactacgaag tcgataagac ggacccgttc gcgtttttta
    16501 acgaaattcc tcccaagacc gcctcgattg tgtgggattt gagctatacg tggcacgatc
    16561 agtcctggat ggaccgccgc agctcgcaga acagtctgaa tgcaccgatc tctatttatg
    16621 aaatgcatct cggatcgtgg aaacgagttc ctgaagaagg acatcggtcg ctgagctacc
    16681 gggaactggc gaactccctg ccaggctatt tggagcagac ggggttcacc cacgtggagt
    16741 ttctgcccat catggatcat ccattttttg gttcatgggg atatcaaacg acgggatatt
    16801 tcgcaccaac gggcaactac ggcacgcccc aggatttcat gtatcttgtg gatgaactgc
    16861 acaggcacgg catcggcgtc attctggatt gggtgccgtc gcatttccct accgatgagc
    16921 acgggctgag ctatttcgat ggcacccatc tcttcgaaca cgaagatcct cgccagggct
    16981 tccaccccga ttggaacacc tatatcttca actacggtcg caacgaggtc aggagctttc
    17041 tgatcagtag cgcattattc tggctggaca aatatcacat cgacggactt cgtgtagatg
    17101 cggtcgcgtc catgctgtat ttggactact cacgacagga agggcagtgg atccccaatc
    17161 aatacggcgg acgagagaat gtggaagcga tttctttttt gcgtcggttc aatgaggagg
    17221 tgtatcggaa ttatcctcaa gtgcaaacca tcgccgagga atcgaccgcc tggccgatgg
    17281 tgtccaagcc gacctatctc ggcgggcttg gcttcgggct caagtgggac atgggatgga
    17341 tgcacgatac gctgtcgtat atgagccagg attcgttttt caggaaatat catcacaata
    17401 aactgacctt ccgcatgctg tacgccttcc aggagaattt tgttcttccg ttgtcgcacg
    17461 atgaagtcgt gtatggcaaa ggctccttgc ttgggaaaat gccgggtgat ccatggcaaa
    17521 agttcgcgaa tttgcgcctg ctgtatggct acatgtacgc acagcctgcg aaaaagctgc
    17581 tgttcatggg cgacgagttc ggacagtgga gtgaatgggc gcacgattcc agcctggaat
    17641 ggcatctgtt ggagcaggag aggcatcgcg cactgcacac gtgggtgaca gacctgaacc
    17701 ggctttaccg aacagagcgg gccctccatg agcgggatac agatccaacc ggcttcgaat
    17761 ggatcgattg caacgatgcc gaagcgagcg tgatcagtct gcttcgcaaa ggcgcatcac
    17821 ctgacgacct tctgtttgtg gtgtgtaatt ttactccggt gccgcgaacg aactatcgga
    17881 tcggggtccc tcgcggtggc ttctggcagg aagtgctgaa cagcgatgcc acgttgtatg
    17941 gcggaagcgg gtgggggaat ctcggaggca tcgaggctgt gccggtgccg ttgcatggac
    18001 ggagccactc cttaacggtc accttgccgc cgctcgcgac gttgttcttc agactggccc
    18061 gctagaagag gcgtacaacg actccttctt gaggtccgag ttgcacgcgg cgcggatccc
    18121 agacgggacc tcgcttctgt gtatccgtcg agagcagcac ctgaccgctg gacgagccat
    18181 ccggcatcgt gatggctaca gtccgttctg aaaaattcac ggcgaccagg atccgttcgt
    18241 gcgcgccgtt ttccaggctt gccacccgtt catagagaag acactccccg gggccgccag
    18301 ggacgagacg gcagtctccg cgggtcaagg ccggtgatgt gcgccgatga tggagcagtt
    18361 gccggtacag tgaccagagt gactgcgggt tactggtcga ttgggcgacg ttgagctgcg
    18421 gatagtccgg tgacacggga agccagggcc gctcggttga aaacccggcg tgacgggatc
    18481 cgtcccactg catcggcgtc cgcgcaggat cgcgtcccgt gcggaagggc cagaagcgct
    18541 tcgtataggg gtcctgcaaa tcacggtacc gcagccgtcc attgcgcatc ccaagttcct
    18601 cgccgtaata cagaaaaggt gtgccgggca tcgtcagcag caagagcgcg gcgagtctgg
    18661 ctcgagcctt ggcgtcaccg cggtgaccat agcggtcaat gtgccgcgat tggtcgtggt
    18721 tgctcaggac gatcgtcggc catgcaccct tgggaagagc cgtgacgtgc tctgacagga
    18781 accgacggaa ggtttccgca ctccacgtgg acttcatgac ccgaaaatca aacacgagat
    18841 gcagttcgtc gtctcccgtg ccgtagaacg aggctggtcc tccgggcgtg tcggcagctg
    18901 cttctccgat caagaccacg tctcgatagg ccgagacgac ttcgcgtaac tcgcgcatga
    18961 tctggtgtgc cagttctcgg tcccgatcat gaatgtggtg ctgtctcgtg tagccgcgaa
    19021 ggccgaaccg aaacggatta tcgggccacc gcatgtcctt gcccagccaa ttgatcgcat
    19081 caagccgaaa cccatcgatt ccgcgatcga gccagaattt gacgacgtcc cacatggccg
    19141 cacggacggc ggcattctgc caattgagat ccggttgttc cttcaa
//
LOCUS       DASSCQ010000008        44987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1490149, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000008 DASSCQ010000000
VERSION     DASSCQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 44987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44987
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490149"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFH05_00475"
     CDS             complement(<1..335)
                     /locus_tag="VFH05_00475"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET6676720.1"
                     /translation="MVRVVLRRYALVSIHLRGRCSTHVRVRTIHRIDFRRRNANHVTR
                     FSRCETIPSLRVSRGLSMTDASDSTAVRARISVSGRVQGVGYRAFTVRNAVQRELVGG
                     VKNCEDGRV"
     gene            396..1907
                     /locus_tag="VFH05_00480"
     CDS             396..1907
                     /locus_tag="VFH05_00480"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HET6676721.1"
                     /translation="MRWSSVAAKIGTCGSQRLRIVRGHMAFRATDLKLVLEEIAPVLI
                     EGWIQKIYQPATRVVVFEFRARGATYRLLVSCEPETARLHLVQHRLRNPPSPPPFCQF
                     LRAHLDGARLQMMHQVSEDRVVTMEFTTKSGPRTVVCELTGHAANILVLDERHHVLRD
                     LNHQRDRVGQHYQPPASQPPPLDKKVTARNTAKTEDYRFPVSAAIESDYREQDARAAQ
                     DRVRLEQLRSLRKMLKKTRRRIDAWREDLAKATRYRDYARYGELMKANLSSITPAKDQ
                     VTLVDYYDESLPEVTIPIDRCKSAVNNMDDYFKKHRKYMAAERELVPRIAQAEGELNA
                     LERQIASIEQGTWTPPAEGRPARSNPVVKRDTSAPARGPFRRFTSGDGLPIFVGRNAR
                     ENDQLTFGLAKNDDLWLHVRGASGSHVVVRLSKGSDPPPETLRDAATLALLYSDLKKS
                     GKGEIVYTRRKWVKPAKGRALGAVIVTQEKTIHVRLDPTRLDALKARQERSLS"
     gene            1992..2501
                     /locus_tag="VFH05_00485"
     CDS             1992..2501
                     /locus_tag="VFH05_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6676722.1"
                     /translation="MSPSPPSATAGPTVLVVDDDATVVAYCKKILQEAGCSTLSASGS
                     SEALKICKQHGGTIDLLITDLVLPPPEFSFASSENEFPHVHGHELALRALRMRKDLHV
                     ILMSGNPDTDLAGYGIRKDTLPFLSKPFDPPAFVSLVKQTLHTPPPTPEMLLKQPSPG
                     TKGADEWFD"
     gene            complement(2587..2919)
                     /locus_tag="VFH05_00490"
     CDS             complement(2587..2919)
                     /locus_tag="VFH05_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6676723.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFGAHGAVASARIITDKFTGQSR
                     GFGFVEMSSDSEAQAAISALNGSDMGGRTLTVNEARPQEPRAGGGGGRGGFGGGGGRG
                     AGGKRDRW"
     gene            complement(3175..3966)
                     /locus_tag="VFH05_00495"
     CDS             complement(3175..3966)
                     /locus_tag="VFH05_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6676724.1"
                     /translation="MKRHRVVALVARHLYLYRRSLPRIMEIFYWPFLDLVIWGFITMY
                     LAKYQPQVPGFVTFFLGALILWDMLFRSQQGITISFLEELWARNLMNLFASPLSPAEF
                     LAATIAMSIFKVTCVSIVMGICAWLFYGYNVLIMGLWLLPFVVNLVITGWVIGVLTTS
                     LIMRFGQEAEILAWSLVFLFQPISCVFYPLDVLPYWLQVVAWMNPAAHIFEGMRDVLR
                     EGTVPFAHLAWAGGLNVALLVMTIILFYKTFAYCKVQGSLVRVGE"
     gene            complement(3963..4379)
                     /locus_tag="VFH05_00500"
     CDS             complement(3963..4379)
                     /locus_tag="VFH05_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676725.1"
                     /translation="MPSAVINFAVGVLMGGLALSAAWGLFWLTVGAIGLWRGTCSWRV
                     VTNSLVVGGAPLLLAWALVSVSGAVLPNMAFVGGLLVIPFILAGFAMRPVPGGHRAGA
                     AMLQGIGRLIDELLGKHHACGGCEHEHGPDLTGGSA"
     gene            complement(4397..5125)
                     /locus_tag="VFH05_00505"
     CDS             complement(4397..5125)
                     /locus_tag="VFH05_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6676726.1"
                     /translation="MTTPVLRVSHLRKQFGDFTAVEDVSFSIEPGEILGLLGPNGAGK
                     TTTIHMLLGLITPTAGSIEVFGMELAAHREQILQQVNFSSTYISMPMALTVEENLWVV
                     ARLYGLSEIYRRVDDIVKKLEMEEFRHKVTRKLSSGQMTRLTLAKALLTEPKILFLDE
                     PTASLDPDIAHKIRALLKEERRSAGLSILYTSHNMREMEEMSDRIIFLQRGKIVAEGT
                     AREIIARFGQADLEEVFLKLARER"
     gene            complement(5145..5852)
                     /locus_tag="VFH05_00510"
     CDS             complement(5145..5852)
                     /locus_tag="VFH05_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HET6676727.1"
                     /translation="MSWTSRATIALVIVAGAMALGAVAIPLTNHPAFCASCHTIKPSY
                     DSWAKSSHKDVTCVACHVRPGIEGWLQDKAWAGTKDVAIYLFGTPTDPHNLKAQVDSQ
                     VCLSCHQHILRTSEVAPRDLPVPVKDVGLVMSHRKHMEAFGVRGQKEGCTTCHSGVVH
                     DQPIKGYPIVLPRGHVSTDNKPWHPEYPEGSYLRTRALNDCFRCHDGKAQHEGRILDR
                     KCETCHLPDKIKDFLSF"
     gene            complement(5836..6360)
                     /gene="mobB"
                     /locus_tag="VFH05_00515"
     CDS             complement(5836..6360)
                     /gene="mobB"
                     /locus_tag="VFH05_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HET6676728.1"
                     /translation="MDVPIVCFVGRSNSGKTTFIERVIPELVRAGYKIATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVILSKGSMAMFADVSDQMNVEDVRSRFLDGSYDLIIAE
                     GWKHEGYPKIVIIRDQVGEIPISSEGLLAVVSDKPIDLRVPLFDLNDVPGVAALIMKH
                     FPLRRAPSEHELDV"
     gene            complement(6371..7651)
                     /gene="glp"
                     /locus_tag="VFH05_00520"
     CDS             complement(6371..7651)
                     /gene="glp"
                     /locus_tag="VFH05_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET6676729.1"
                     /translation="MSGLTPLEQAQKIVLDATPVLGVEKIPLLDALGRVLGEDVIVER
                     DNPPWDNSAMDGFAVRWEDIRQEHAIQKPVTLTVIEDVPAGKMPTKTVGPGQAIRIMT
                     GAPIPKGADTVLKVEDTEPGHDQVRVFKPEAKGSNIRPQGEDVKKGERIITKGTRIRP
                     GEAGMLAILAKSFIFAHQRPRVAILSTGDELADLDERFSEEKIINSNSYGIAAAVQDA
                     GGIPFLLGIAKDTPAALKEKISHGLTADILVLSGGVSMGDYDFTKAVFRDLGAEMNFW
                     KLAIKPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKMSGHRSYGRPVVQA
                     LFQEKFSKRPDRRHFLRGVLTREDGAFKVRTTGDQGSGILTSMVKANCLIDVPVEVER
                     LNPGDLVSVQLLSGESWANYAEASQNSAHRLSCC"
     gene            complement(7678..8631)
                     /locus_tag="VFH05_00525"
     CDS             complement(7678..8631)
                     /locus_tag="VFH05_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET6676730.1"
                     /translation="MPRELNVISTPTSGGSDACTYMWACAICDENESCQKDKEGHSRW
                     LVAKRMERIEYKVLVMSNKGGVGKSTCTTNLAVSLALKGWHVGICDMDIHGPNIPKMV
                     GAEGQKLKISTSGGIIPFQAYNLKIASMSFLLQNSDDPIIWRDAYKYEFINQLLGGVE
                     WQDLNFLLIDLPPGTGNESVTTVDLLGQVSGAVIITTPQEVALLDSRKSVTFCKDSEV
                     PIIGIVENMSGLECPHCHQHIDVFRKGGGEASAQDMGVPFLGRIPLDPDVVIQSDAGE
                     PFAMFNSDTPTAEAYHEIANKVETFCKKSGSLVNVAPRQHR"
     gene            complement(8707..9633)
                     /gene="tatC"
                     /locus_tag="VFH05_00530"
     CDS             complement(8707..9633)
                     /gene="tatC"
                     /locus_tag="VFH05_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET6676731.1"
                     /translation="MLNDFNRWLQDSVFKPLEDKKMPVMEHLVELQVRLTRAVIATAV
                     VFVGTFFYADTLVKWLRVPLQNMFVPGSLSWVPTDLPTVPFVFLAPAEALWQNVKVAG
                     LFAIVLATPYILLEVWQFVVPGLHTQERRFVGPFVALSTVAFYAGVGFSFFFVLPFAL
                     NFLVSYGVSAGFIPQLSIAQYVGFALWFLMVFGLIFEVPLAITLMAKLGWVDAPFLKR
                     YRKWALLGAFIVAAILTPTPDPFNQCLMALPMYIFYEVGIISAGFFNKKKAQEKPDNA
                     LSPVGPKAMAPAMSGAGDGEYVGVPTAGGRRR"
     gene            complement(9693..11099)
                     /locus_tag="VFH05_00535"
     CDS             complement(9693..11099)
                     /locus_tag="VFH05_00535"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET6676732.1"
                     /translation="MAQGFSEIVKKVTPAVVNIAVTGGSEGGRGRRPLPPGPFGGPPG
                     GGPPGGGPPDEPGGELPTPPPMPPPGPHGRPDQSAGSGVIMDPNGYIVTNNHVVEGAT
                     QITVTLSDRREFPAKIIGTDPKTDLAVVKIDVKDLPSVKWAEYEKLQVGDLVLAIGSP
                     FGLSSTVTLGIISALGRGNVGIADYEDFIQTDAAINPGNSGGALVNMNGDLIGINTAI
                     FSRTGGSEGIGFAIPSSIAVDIVDSLQKTGKVVRGWMGVAIQEITPALAKSFKLPEQR
                     KGVLISDVNENGPSHAAGVKRGDVVVSFNGKEVQSVSQLRNLVARTVVGKDAQIKILR
                     EGKEQTLSVKVAERPSDEILAKKESAPPKEQGETIKPPDNVLASLRVQSLDNAMMSQL
                     NIPAKTAGVVVSSVEPGGPAEAAGIQRGDVIQEVNHEVVKTLDEYQKAAHKIKKEELA
                     VLLLSRQGNNLFVAVNPK"
     gene            complement(11384..12166)
                     /locus_tag="VFH05_00540"
     CDS             complement(11384..12166)
                     /locus_tag="VFH05_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676733.1"
                     /translation="MRRIVDVSNFLLVAVLLAACASATPPSRLGDYVSSDHRANDEAF
                     SRIEQRPLKAGLLLVSDTAEPGAAPNLPDEALVRLSETLKQDIARTIPVTITQVLPAD
                     QIRPQPNGDWTQFAELGRLHGLEYLAVVVLSSTEQEYPVTLFLGWTTHAQPGFRRDNW
                     SLLEFALLDLKNNVTLMQAEGRGWATLDRPSAPGISQWYPVIYLRPQDPERRIWPPTY
                     EGAPNTLRVVSFDQAEKRLALKLQNSWVGQLEADAAERRTAS"
     gene            12348..13163
                     /locus_tag="VFH05_00545"
     CDS             12348..13163
                     /locus_tag="VFH05_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HET6676734.1"
                     /translation="MFGHHSTDFPELSIVIPAHNEAGRILPYLLQITQYFRQHNRTYE
                     LLVVDDGSTDATASVVEDVSRANPAVRLLRLARCRGKGAAVRHGMQSAVGSLQLFADA
                     DGATPIEEFARLERALADDVDMAIGSRALASRLPEFAVQARFHRTLFGMLFNAAVRRS
                     GIRGIADTQCGFKLFRRVIAQDLFAHASIDGFGFDLEVLYVAQQRGYRIAEVPVNWSD
                     QPGSKVRVLKDGIAMLRELATIKRNDHCGRYSLQPNSTGWGNVSADRLRIPVR"
     gene            complement(13129..14709)
                     /locus_tag="VFH05_00550"
     CDS             complement(13129..14709)
                     /locus_tag="VFH05_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676735.1"
                     /translation="MMNHGYAAHDDPQTAAAVRQSLDTPVLNFLLLMVIVNFALQPLT
                     EPDFGWHLRTGLDLLHNGFLLPHSDPYSHTMPDWAWVEHAWLTDVVLGAVYSFGGGLG
                     IILLFACVTTGAWLIGSATARAGVVFRRLACAISLWVALPYLGARTQLVTLLGLAVLL
                     FLLKRWQDGVSSARWWIPPLFFGWANLHGGFLAGLMVLGLIIGTTAFLRWLTERRIVS
                     ARKYDEPLLSWDDLKRLTLLTILSVGLTLINPYGWRLHAEIVDSLSDRFMLETLQEWQ
                     PLSLSGVAGRRYALYLAGLTSFMLLWYRRTEPVRWVIGGVFLALSLRHMRNIPFFLIY
                     SLSTCADVLAQGFHTLERRWLWRRLTARQGSLAAAVAMGAVLFWLGAEHLQQVVFSGT
                     RPAEYFRRTSYPIEAVEWIRMNRDLIGRRLYNDYAYGGFLLWWLPETRVFIDGRMPAW
                     RCGERRIFPDYMALTGADPDLTVLTKYSIDWAMVRKQTPLDGALAQGHGWTRLYGDEK
                     VAIYRLLPIELESSGDPR"
     gene            complement(14729..16453)
                     /locus_tag="VFH05_00555"
     CDS             complement(14729..16453)
                     /locus_tag="VFH05_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET6676736.1"
                     /translation="MRILGLSNMRDAAAALVENGQIVAAAEEERFVRIKHVTALPVHA
                     VRFCLETAGIRLADVDAVAVPWKYWQVGRRAALAIGSMLRSPQLFMAKGTRSLERLTH
                     EWRELAFLRSSLSRIVDGTPCPAPVFLDHHLCHAASAMLVSPFERAAVLVVDGASESH
                     TTMLGQADRHHIRVLNRIPLPHSLGQFYAAVTAYLGFKPDFDEYIVMGLAAYGEPRVA
                     PVLREKILRVLPDGEFCLNTRLLDFHLARRRVFSSEFLRLFGAARRPDEAITQRHQDF
                     AASAQLVLEEVLLYVARHLKVLTKEKQLCMAGGVAYNCVANSRVWREAGFQDLFIPPA
                     AGDSGAALGAALWLTHRKGREVDRTVMRAASWGPKFSEEACRAALEGAGLMVERLADS
                     SLCEKVAGELAAGRLVFWFQGRMEWGPRALGYRSLLADPRREDMRALINLKVKFREPF
                     RPFAPSVLEERAAEFFDLPGPSPFMLCTAPVRSAMKGVIPAVVHVDGSARVHTVDGHS
                     NPRYRRLLEAFSDKTGVPVLLNTSFNVNEPIVCTPDDAVTCFLRTEVEWLVMENLLAR
                     RPMSRTER"
     gene            16688..18385
                     /locus_tag="VFH05_00560"
     CDS             16688..18385
                     /locus_tag="VFH05_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET6676737.1"
                     /translation="MEFSPSLLESKAEYTDNCGHIQIVDIGSTLQDVLLEAANRTFTS
                     VVRPGSGAKPDVVVRVNLIQSTFKLRMDGVYDRAETEIQLGGLVSYVDQAGTALGEQE
                     VQVVQKGRVRIMPVQKNCDYLLDSFIQDATHDFAQKFAVTTRAKLGAPGAPLAAAAAP
                     AAIPVPAPTRPVPSGAAPLSFKATVLDDNSNLIFEGGERIRVRVDVVNTGAQDLQNVT
                     ANLSGSPQVLAQFPTTTLSAGRLQPGQSRSLEFVATLPQTVQQQKAELHVAVFDPAAG
                     SSPSQALSLSIQPTGTNTDDVDQVPAMPVGFKRPHTYLISIGIGSYRDVQGGARKYAA
                     SDAELLTTYFQSLGGLPASNVRLLQDWKALKPDIDEALLDWLPGHMNKDAVVIVYFAG
                     LATVAPNGDVYLVPYDGNTTNASRSYPLKDLDAALGRLKAKQTLLFFDGSLTRLGADS
                     RSKTVVPQWSPSGSSTVHVVAMNGIGKAVEDEGHRHGLFTYYLLRALRGESDVNRDGE
                     VTLGEAIAYVGQKVSWASKTHYGQEQRPAVFPSLKTTDPISGLALAKLVAIKAAEQP"
     gene            complement(18446..18895)
                     /locus_tag="VFH05_00565"
     CDS             complement(18446..18895)
                     /locus_tag="VFH05_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6676738.1"
                     /translation="MGYFSKFLGVAAAVMLLSATVVGAEEKDPLKPRVPPDQIADAKA
                     LKNPVANTPESVAKGKALFEGKGTCFNCHGKEGKGDGPAGAILNPSPRNFTNCKFHKK
                     RKDGELFWVIKNGSAGTGMVSLIPAAITEEEAWTIINYERSFCKGDE"
     gene            complement(19292..20248)
                     /locus_tag="VFH05_00570"
     CDS             complement(19292..20248)
                     /locus_tag="VFH05_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET6676739.1"
                     /translation="MDSIGDFFKQVRETKGLTLEEVASKTRIRTDFVKALEEGNFAKL
                     PDQVFARGFVRSYARSLGLDEEDAIHRFIQSAGAFYEKQDERERLKVRQAEEERKRRA
                     NRKAVAIAIGLAILTLVILLSREQSSLLVRRTSQSEPSAMAGKRAAPPESGRDVAKSD
                     LPARPEVEAQPGAVKGKPSELPTPPPLARADASSSRAAAPAPPPPPVQPHATVAAPVP
                     PPPATEGPLSALTLQGQANTDEPLVLDLEATELSWVVIQIDGGSPQEALLRPGEKAKW
                     KGQDQFTLTLGNAGGVKAELNGKPQKPFGPSGKVARDILLKR"
     gene            complement(20259..20939)
                     /locus_tag="VFH05_00575"
     CDS             complement(20259..20939)
                     /locus_tag="VFH05_00575"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676740.1"
                     /translation="MNRLPMPPQSYAGFVALLMLAVVLVGACGTSDEMKQKSKGYYQE
                     GMASLEHDRQKSYVSFQKAVQLDPRNKEARYGLGHILVYQGKLSQAESEFREAIKIDE
                     NYSEAHTYLGQVLASQDRWDEAIAEFRRALSNPLYSTPDLARYQLGKALAHEGDYQGA
                     MEALEDALVVNPPNVPPAMLQLELGRVYYKLGFERRAKDALTKVTTLDKNGSYAAAAK
                     ELLARPKP"
     gene            complement(20936..21850)
                     /locus_tag="VFH05_00580"
     CDS             complement(20936..21850)
                     /locus_tag="VFH05_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6676741.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEATEVLGGSATYFSTSASYFTAVD
                     LIAVVGEDFPSQHLAFLKSRNIDLAGLERRSGATFRWRGEYTHQLNEAHTLDTKLNVF
                     ETFRPKIPESYRSPDVLFLGNIDPELQLDVLNKLPRPPLVACDTMNFWISGKRDALWR
                     VLEKVDILIINDGEARALGGDSNLVKVAASVLARGPKHLIIKRGEYGVLMFNENQVFG
                     APAFPLEDVRDPTGAGDTFAGGFLGYLAATGNRSVESFKQAIIFGSVMASFTVEAFSL
                     DRLRVLDYKEIQDRFRAFKRLTHFEDLP"
     gene            complement(21854..22753)
                     /gene="mtnP"
                     /locus_tag="VFH05_00585"
     CDS             complement(21854..22753)
                     /gene="mtnP"
                     /locus_tag="VFH05_00585"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET6676742.1"
                     /translation="MTKLTRASRADIGIIGGSGLYDMEGLHQVKRVDVRTPFGSPSSA
                     IVLGELDGVRVAFLSRHGVGHRYNPSEINYRANIYALKSIGVRRVISVSAVGSMKESI
                     KPGDVVLPNQFIDLTKRRLSTFFDGGLVAHVAFGEPVCASLSASLLGAGRSVGATVHQ
                     GGTYVCIEGPQFSTKAESQLYRQWGVSVIGMTNLPEAKLAREAELCYATVALVTDYDC
                     WHETEECVTVEAILATLCQNVALAKRLLAAALKSAASATSCECQRALQHAIVTAPDKI
                     PLALRRRLTLLIERVLSPVKGKR"
     gene            complement(22768..23796)
                     /gene="miaA"
                     /locus_tag="VFH05_00590"
     CDS             complement(22768..23796)
                     /gene="miaA"
                     /locus_tag="VFH05_00590"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET6676743.1"
                     /translation="MTTCSHLTMPSLSERQLRRKPLVVILGPTAVGKSRIAVEVAKYL
                     GSEILTADSRQVYRGMDVGTDKPTLLAREGIVHHLIDLVDPDEPFNAGLFRRHAAAAI
                     DECYRRRMLPLLVGGTGLYVRTLLQGLCEAPPSDPCVRARLRHEAYEQGGEQLYARLA
                     LVDPLTAAKLHPRDTPKILRALEVYLQSGSPLSSFQSKHGFRERPFSALVIGLNRERD
                     HLYRRIEERIDWQLSHGLIDETQTLLRRGYRRDSPAMKGLGYRQVASYLAGECNWNEM
                     VNEFKRDTRRFAKRQMTWFRREPELTWLMIEESEPAEQTVSRVVDLIHPFLEGLDGDI
                     DTLVPHSW"
     gene            complement(23793..25574)
                     /gene="mutL"
                     /locus_tag="VFH05_00595"
     CDS             complement(23793..25574)
                     /gene="mutL"
                     /locus_tag="VFH05_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET6676744.1"
                     /translation="MPAARCSPTIRVLPADVVSRIAAGEVIERPAAVVKELIENSLDA
                     GSRHITVDIRDGGTSLIRVTDDGQGITRSDIPLAFERHATSKLTSDAELTSIGTMGFR
                     GEALPSIASVSKVLVATATNEDLVGAQLSLIGGVPQQLVDAPAVIGTKIEVSDLFYNQ
                     PARKKFLKSIPTEFSHISTVVQQAALAWPFVHFRLTHNGQEVLNHAAVASDRDRILQV
                     YRQPFLERTVEVRGRIAGCTISGVMIDPVHARSSRAPQDLFVNRRPVRNAAVFHAISE
                     GYGSLLAKGHHPTYVLFLDLDPERIDVNVHPAKREVRFADQEGIHRFVRQSVRHAFSG
                     VERKAVLRLTDDPATATAMASFTDGSSSSASSGWRPAEQADQRLASRPDSLFAAERTG
                     AHPASKDVTITPLGQMHQTYLIAQVGLELHVIDQHTAHERVLFQRLWRRWMARDMAIQ
                     PLLIPEPIDLTVAQYTLMQKYAKDLELLGLVLEPFGTNTVVLRAVPVGVGPVDGASVV
                     RNVLEDLAEWASVSSVDARVQAVLATLACKSAVQAGRPMALPEIEQLVRDWVDEGVMS
                     TCPHGRRTAFRLSADELDTLFGRTGWS"
     gene            complement(25602..26369)
                     /gene="ybgF"
                     /locus_tag="VFH05_00600"
     CDS             complement(25602..26369)
                     /gene="ybgF"
                     /locus_tag="VFH05_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET6676745.1"
                     /translation="MHDGMMMLAVGCVTVTALTVSGCAKHADFVELRDHLATVAKSQD
                     QDQKRLDALQRRMESMERVKDVEAPKTRLEDFNARLQKLEARVAKLDEGATASPALKS
                     EAAPTEPARTIKPQRAGSEGSSIVPNVPAITPTSAFNLSYNDYLNGKYDLAVSGFQRF
                     VKDFPGTSLTPNAHYWLGESYYQQKDYARAMQAFEYVSTEFPGNEKVPAALYKLGLAA
                     AETGDLVKSKKNLKRVIEEFPTSDEAKLAKNKLADIR"
     gene            complement(26372..27934)
                     /gene="ybgF"
                     /locus_tag="VFH05_00605"
     CDS             complement(26372..27934)
                     /gene="ybgF"
                     /locus_tag="VFH05_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET6676746.1"
                     /translation="MNIGWNVASVGTVVCVFSAALLHGCVAQQADLKSAERRFKESSD
                     ALAQRGAQQRQELEELKSQELPKLRGELERAHHQAQEIQRAQDDLKQRSAVLEQQTRK
                     LEQLSGKLDADTANRYAQVRESLNAQDLKNKADRDQLRVDVNTRLDDVNHQMEVLRKD
                     IIDAVQKTNTAFAKSLDVKLEEQRKALADNQARTEQLSAKFAQFNQALAGFRESLSSL
                     GDRIAQEEQASKTLAAKVEADTKASTAHINETTRSMSGHLGEVNKSVNAVAQKLAARV
                     DEQMGEINKTVTKLDGRVAEQDRRIDGLTKSLDHVTQEVHTRGTSPKAGQRQQSAGTK
                     SMQRSSAVGPAEGVSHHEAEPVAAARQEPETVNHVADRTVAADEPAEPVALAAPVRPP
                     ERSDRAEYERVLAFFKEGNLEGSREGFSSFLTEYPNSELAPNARYWLGESHYGKRDYK
                     QAIDSYDRVEMDYPQSEKVPAAILKKGFAYLALKDKKRASSAFKQVVTLYPKSPEAGK
                     AYDKLNQLKELR"
     gene            complement(27960..28682)
                     /gene="pal"
                     /locus_tag="VFH05_00610"
     CDS             complement(27960..28682)
                     /gene="pal"
                     /locus_tag="VFH05_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET6676747.1"
                     /translation="MGTTCFKIAMALVASVALMGVSGCSKKSVQSGGDTQSSHQGSAA
                     KSGSTEGSKSGVGNFPDTSMQSGGSGLRGLDKNPSEEHVASGFGASPAGASAGMGGSL
                     LAKADPSDAASRQLDEMRAEQAASASAGLRDVYFGYDSWTISEDQRQALTRDADWMKS
                     NPVAMVKVEGHCDERGTSAYNLVLGEKRAKAVRNFLVELGISANRLSVVSYGKERPSC
                     AEHAESCYQQNRRGHLVVKSGK"
     gene            complement(28749..30092)
                     /gene="tolB"
                     /locus_tag="VFH05_00615"
     CDS             complement(28749..30092)
                     /gene="tolB"
                     /locus_tag="VFH05_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET6676748.1"
                     /translation="MNRRIVLSLVLSGLCLLAAAAGILESDAADVFLEATRPDFQKIP
                     LGVVSIENNGGPDWLGGRLEEVLKKDVKRSLVFELMDLPSLGIKVRDVGNGHGNGSKA
                     VFKQAAENGVSVLVWGKAGLKKGEKEDDVNMEGFVYDSGSDEIVGGKRYAGSPSVVRL
                     MAHRFADELVFRYTGEPGIARTKIAYVSAHGAARELFVMDYDGYDARQLTADGFLNLM
                     PRWSPDRRFLVFTAYRDRNTQDIDMIELATGKRWTLVSYGGLNITPALSPDGNFLAFA
                     SSHEGNSELYRLDTRTKAVQRLTTNPAGDLSPTWSPTGRELAFVSDRGGGPQVFLMSS
                     DGSNVRRLTFEGDYNAAPAWSPRGNWIAYVCRTPRKEYKLCVISPDGQKRMQLTTGGG
                     IDDSPSWSPDGRHLAFSSTADGKSHIYMIDADGKGLERLTFEGSHNSAPSWSPAS"
     gene            complement(30089..31084)
                     /locus_tag="VFH05_00620"
     CDS             complement(30089..31084)
                     /locus_tag="VFH05_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HET6676749.1"
                     /translation="MVARAQVQSAFWFDHDEQDRFLARLGRTLTISLVLHLVALLIAM
                     GIRLAPHGERPLASVQVNLVSLPTPMKPVEQSRPVEPVKRLEPKPVPVSAPPVRMPAP
                     AAPPVSTPARTSVAKDILKDLELPPDAPKFGELTPVKSVAQPRPQVQAKVHVPDLPRI
                     PDIMPDPVTKTPQRTSISEELDRELEEELKKVKEFKPAAKLEIPKEAPVKAVPHQEAA
                     LPHVTAPQTQLKTSGSSGTNPFWGRVEAIIRSQWEPPPIDVTGQTYSAVVRFRFYRNG
                     TVKDVVIQQTSGNSYFDMSGQRAILKPRQFPAFPADMSDAYQDVEMVFRVGEIAG"
     gene            complement(31113..31565)
                     /locus_tag="VFH05_00625"
     CDS             complement(31113..31565)
                     /locus_tag="VFH05_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6676750.1"
                     /translation="MMFESRQRRFMAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDIN
                     LPKSASNTIKPEMRTVLTIEKDQRLYLDKDPVGVAQLEQKLKVLKHEHADVSLYLRAD
                     REVPYGIVVQVMDGVKKAGIEKIGMVTEPVGPEHIGGASIPRGPSRKS"
     gene            complement(31562..32254)
                     /locus_tag="VFH05_00630"
     CDS             complement(31562..32254)
                     /locus_tag="VFH05_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET6676751.1"
                     /translation="MFQIGLTGLLGSLGAVSKVVLLLLFVFSVVCWAIIFLKWRAFSA
                     ADRMDHQFMALLAKAKDLDDLCRQVRRMETSPAAILFEGVMDRVAGLRGQDGAGVADR
                     QLVERTAAHLSHSQLARLESYLPFLATTGNITPFIGLLGTVMGIIDAFREIGAQGTAS
                     IAAVAPGVAEALIATAAGLFAAIPAVIAYNYFLSRIRRTAFRLDTVTIELMTSLSIPT
                     IKAKPIPVGAQR"
     gene            complement(32453..32531)
                     /locus_tag="VFH05_00635"
     tRNA            complement(32453..32531)
                     /locus_tag="VFH05_00635"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32493..32495),aa:Asp,seq:gtc)
     gene            complement(32646..32721)
                     /locus_tag="VFH05_00640"
     tRNA            complement(32646..32721)
                     /locus_tag="VFH05_00640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32686..32688),aa:Val,seq:tac)
     gene            complement(32806..33759)
                     /gene="xerD"
                     /locus_tag="VFH05_00645"
     CDS             complement(32806..33759)
                     /gene="xerD"
                     /locus_tag="VFH05_00645"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET6676752.1"
                     /translation="MAIIAEMETDALVERYLNHLRVEGGLARNTIEAYRRDLAKFQRY
                     LSERKIGLRKRLPPDTVRSFMGSLHAVRLSPVSSARCLSAVRGWLRFLRQEQIIDDYT
                     ALDAAPVRRGIRLPKTLSAREVTALLESPAQSTLEDQRDRVMLELLYATGLRVSELIS
                     VETLHVNLEVGYLRVTGKGSKQRIVPMGEPARRVLLEYLDAIRPRFLRQRASRFLFVS
                     RRGTPLSRQAFWKSLRARAQRAGLTKAVSPHMLRHSFATHLLGGGADLRSVQTMLGHA
                     DIATTQIYTHVERERLKEVHANFFPRQRRGKDGRAVTEARK"
     gene            33848..35845
                     /locus_tag="VFH05_00650"
     CDS             33848..35845
                     /locus_tag="VFH05_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HET6676753.1"
                     /translation="MRTSHDRHFIRRALRLPDLHRIQLPIAACVALTLALVTFAVPGR
                     HPVAAAETAKRAPASHPSLNQAKRLIDAGQAEEAITILRRFLGTNPKPEFLDDTYLLL
                     GAALYRGQQYPEALKYLQQLQTEFPSSEVIDRGKLMLARTHAAMGNPDLALPILTGLR
                     ALSQDDVTKREAVHLSGELYFQKKEPARAIQAWLDEIALSSDEQGEEIRNNITEVITE
                     SFDKKALERIREAYPKQFPGDLAALRLIDLYMGRGEDHQASRQIQQFLAHFPTHPQRG
                     RLSEMLEALQTKLKSSQYVMAAVLPLSGKLSSFANDVLSGVQLAVEAGSERGGGPSIG
                     LLVKDLDASHASFLDDFSHLLFNDRPIAVIGPLLSKNLPVMAELAERSHTPLLTPTAM
                     LPNVRRLGSFTFSTAVTYQLQARKIAAYATVDLGFRRFCILHPDTAYGREMARLFAQE
                     ARQRDGEIIAIESYKEGDSDVGAQLKRMKAEDLKKYGLAVQVDPLKVGGKLTKLDKKV
                     LYTPGFDAVFIPGRAADVGLIAAQLNFHDMKVPFLGSNGWNAPDFARTADQSIDGAVF
                     VDGFFAESPNPSIQDFVDRYKKRFQSAPTLFAMQGYDAAKFVVDAIKKGATSGDALRD
                     YLTSQQDLPALAGPAAFAQDGTLNRPLFLIQVKRGRFTQVD"
     gene            35958..36701
                     /locus_tag="VFH05_00655"
     CDS             35958..36701
                     /locus_tag="VFH05_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6676754.1"
                     /translation="MNDVTQILHAVSYKALPLLFAIVLHEYAHGWVANRCGDSTAKLQ
                     GRLTMNPLAHIDPFGTIIVPLICLMLPGSFLLGWAKPVPIDPRNMRRPRRDMAIVAAA
                     GPGMNLTLAVASAGLLALLLSVDPSLMTSDSRTDTLETAPSSAFRFLLPVAVMALYSV
                     LVNVFLAIFNLMPIPPLDGGRILLSLLPPRPALALARLEPYGMMILVGLLVFDRELRV
                     IHTFTSTFASGLSGTLLSTAIGFSAGASP"
     gene            36698..37690
                     /gene="trpS"
                     /locus_tag="VFH05_00660"
     CDS             36698..37690
                     /gene="trpS"
                     /locus_tag="VFH05_00660"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET6676755.1"
                     /translation="MTSRPKRVLSGMQPSGLMHLGNYLGALQNWKHLQTEYECYFFVA
                     DWHALSTNYADTSRIREFVRELLIDWLAAGIDPNRSIVFIQSRVPEHAILHLLFSMII
                     PMSWLERNPTYKEKQEEIKEKDLTTYGFLGYPVLQAADILLYKPDFVPVGKDQLPHLE
                     LTREVARRFNDLYKHSVFPEPKEHLTKFPKVPGTDGRKMSKSYGNTINLSDTEPVVRQ
                     KLKTMVTDPARVRRMDPGNPELCPVFDFHKMFSSLPIIEQINRECRTAEIGCIDCKKL
                     VADRVVETLAPIWDARAALLQNPSGIEDLVHAGSQRAATIAKATLQEVTEAMKI"
     gene            37847..38158
                     /locus_tag="VFH05_00665"
     CDS             37847..38158
                     /locus_tag="VFH05_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676756.1"
                     /translation="MESHGGKKLLIAVVLWQLSGDHAWAVDPPCDAFPPAKQLRCVEV
                     WKDLYKEDGPAIAQFGLDQLKRREEGKITAQQHLAQNMDFIKQATERRLARLNERMAR
                     E"
     gene            complement(38227..38733)
                     /locus_tag="VFH05_00670"
     CDS             complement(38227..38733)
                     /locus_tag="VFH05_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET6676757.1"
                     /translation="MRTSARWLALFVLSFAWGPPIVHAEAASGPQSDQVTIADVKVRI
                     SDHGPVVLLQAEGKTIPIFVDLTVAVSIQGALNGEQLHRPLSHDLMHTILDTFGAKVT
                     QSVITLKGGTYYGALSVSFRGDVKVFDSRSSDSIALAIHFKAPIIVGRDLLNSAGVVP
                     EEPKPQDL"
     gene            complement(38752..39390)
                     /locus_tag="VFH05_00675"
     CDS             complement(38752..39390)
                     /locus_tag="VFH05_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6676758.1"
                     /translation="MTELIRKTFAVPPLGCNCSILGDPVTKLAVVVDPGGAAERILRE
                     VQDLGLTVTKILHTHAHFDHFLASGEMKAATAATLCLHKDDHALWDMLELQCRAFGVP
                     YMPVPPPDYWLTDEETVHIGQVRLIALHTPGHTPGSMSFHLPAARIVLTGDTLFRGSI
                     GRTDLWGGDFDAIERSILERLYTLEDATAVVTGHGPETEIGWEKDRNQFVRA"
     gene            complement(39497..40144)
                     /locus_tag="VFH05_00680"
     CDS             complement(39497..40144)
                     /locus_tag="VFH05_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676759.1"
                     /translation="MTGFSLLALTCVLSMGSASLAPAETGPDTSAAVPAEDYPLYDLV
                     VDEKFLTPETKLIVLERFTLTRLHPEQQGPLRPETFHQYEIFDGRLPGELIRDFLVKN
                     QSPSKLDGRFGFGVRYRFVSGDGVDQQEASMPSILVSESPRFMQEEPLDEPPAILDRL
                     AFSRVAYMLGREQALIYVEYNRPTGAGAGFAVWLHRSGAHWRIFDSELVWAGRPQ"
     gene            40253..40981
                     /locus_tag="VFH05_00685"
     CDS             40253..40981
                     /locus_tag="VFH05_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676760.1"
                     /translation="MWCCRHRSILLPLVLTTFLPLSGCKPDESPFRAENDLLKKQLAR
                     QESLVTSLQDGNKVMQQQIDLLNQEIRDAKKAAESARAEAKDAAEQLDKQLIQARKLT
                     ADIKKTAVEQAAQSIQLEQKGAQFEDLPRPLNAVTKTVEEALARNGYQVKVSIKADQK
                     AVIVTDRKVSNPASLEVAGFRNQYVIAVQAQPLNVTRLSVKAEFEKLAQGGRILSVSA
                     EETAEIERRLIGEVSKALSAPSKT"
     gene            40996..41877
                     /locus_tag="VFH05_00690"
     CDS             40996..41877
                     /locus_tag="VFH05_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6676761.1"
                     /translation="MWLAGSVLSLISINANQAASAHKPSPQLAGHLASIAGLAQSAPT
                     VQIPAGEFTVGSKRIDDDPYGLWTQFDDTELPQQRVWLDAYAIDRHEVSLAEYLSHLR
                     QTNQHPADELQKLIWHVITVHSVSDHTLARWPALYITWHEAARFCASKGKRLPTEAEW
                     EKAARGPDGQLFPWGDAAPSSERAMFGQHHVHEIPILAAVDSHPEGRSPYGLHHMAGN
                     VAEWVQDWFGLDYYAYMPGKNPSGPSSGRYKGVRGGSWKSKIIMLRTATRSGFSPDQR
                     SATIGFRCAQPSPNPDP"
     gene            41969..42658
                     /locus_tag="VFH05_00695"
     CDS             41969..42658
                     /locus_tag="VFH05_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676762.1"
                     /translation="MNAASISLTRMVLIGFLVAIVTLVGCATSTGDRSSRTLTGQPCC
                     KSTEATSRRAAIVQTAVRLVGASTIHIKGRRIAYDCAGVTRAIFLEHGIDLYDSAVPD
                     PKANGVKLIHRHVNRHGKLHQGPAVRPGDLVFFDNTWDYNGDGMANDPLTHVGIVERQ
                     EDDGTIVFISRVAGAIERYRMNLALPHVHRTTEGRLLNDYLRRKDFDDPARAAYLTGE
                     LFAGFGSPTGL"
     gene            42754..42918
                     /locus_tag="VFH05_00700"
     CDS             42754..42918
                     /locus_tag="VFH05_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676763.1"
                     /translation="MAKTATYCSECYNKVGRVEDAVVKAAEASGKTPMTGQGTCCKCN
                     KATIVVYYEG"
     gene            complement(42960..43289)
                     /locus_tag="VFH05_00705"
     CDS             complement(42960..43289)
                     /locus_tag="VFH05_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6676764.1"
                     /translation="MERRKHRRVPIQIQGLLLGNSHEVEGHTLDLSLGGAKFASDVEV
                     FPGKTIVVRLVIPGLEEPLSIPEATVQWVDEQTFGVEFQEIPPDELDDLEDLINEFDE
                     TENSGHA"
     gene            43477..43842
                     /locus_tag="VFH05_00710"
     CDS             43477..43842
                     /locus_tag="VFH05_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676765.1"
                     /translation="MRESLIGLHGALILAEQQTYERVSGPVQSVEVLIELLQKDPWFT
                     WLHPIADLLVRMDHLLQDDTCDITGDNVAHLLGEIDSLLHPSVEGEGFERAYYEALER
                     APDVVLAHFRIRKLLTKAA"
     gene            43881..44237
                     /locus_tag="VFH05_00715"
     CDS             43881..44237
                     /locus_tag="VFH05_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676766.1"
                     /translation="MGWMEQVRSSLIAGALMAVWVTQPPLVLGEVPSDNRINAGRDGT
                     RGGTDSLSARQSGAAPILHPERNAHQPTMTPLGVPSPPHQLTPAPVLPFHPNGHLMPS
                     PAVPPNAPLGDPYHSR"
     gene            44311..44739
                     /locus_tag="VFH05_00720"
     CDS             44311..44739
                     /locus_tag="VFH05_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676767.1"
                     /translation="MARTLESSTEAGAGLRHLLMEVRRGLLGLHKALIVAEQLTYERI
                     NGRVGSTGQLLQLVLNDPWFTWLHPLSQLVIRVDELLEEGEELTTVEVEHFLADVRAL
                     IRPSEEGDGFERSYYEALQREPDVIFAHVEVKKLVTKAAA"
     gene            complement(44801..>44987)
                     /locus_tag="VFH05_00725"
     CDS             complement(44801..>44987)
                     /locus_tag="VFH05_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676768.1"
                     /translation="TTPPSVGEDEKKPDKPVKGGKAKGKTKGDAKKANKKHGLDRADE
                     AAGHHGKQGREKARANQ"
BASE COUNT         9650 a        13574 c        12465 g         9298 t
ORIGIN      
        1 tcgacccggc cgtcttcaca atttttgaca ccgcccacga gttcccgctg cacggcgttc
       61 cgaacggtga acgctcggta gccgacgcct tgtactctcc ctgacactga gattctggct
      121 cgaacggcag tcgagtcact cgcatccgtc atgctcagcc ctcgggatac gcgtaacgaa
      181 gggatagtct cacacctcga gaaacgcgtc acgtgatttg catttctacg ccggaaatct
      241 atccgatgaa ttgtacgtac gcggacatgc gtggagcaac ggccacgtaa atggatactg
      301 acgagcgcat accgtctcag cacgactctc accatcggtc gctatggtag aatcgccggc
      361 atcgtcgtga cgggccacgg taggccatcg gattcatgag gtggtcttct gtcgcagcga
      421 agatcggcac ctgcggctca cagcgtctgc ggatcgtcag agggcacatg gcattcaggg
      481 cgaccgatct caagctcgtg ttagaagaga tcgctccagt cctcatcgaa gggtggattc
      541 agaaaatcta tcaaccggcg acacgtgtgg tcgtttttga gttccgtgcc agaggagcga
      601 cgtatcggct tctggtatca tgcgaaccag aaaccgcccg gctccacctt gtccaacatc
      661 gcttgagaaa cccgccgagt ccgccgccgt tttgccagtt tctgcgcgcc catctagacg
      721 gggcccgact ccagatgatg catcaggtga gcgaggacag agtggtcacc atggagttca
      781 ccaccaagtc aggacccagg accgtcgtct gtgagctcac aggccatgcg gccaatatcc
      841 tggtgctgga tgaacgtcac catgttcttc gcgatctgaa tcaccagcga gaccgtgtcg
      901 gtcaacacta tcagccacct gcgagccaac cacccccctt ggataagaag gtcactgctc
      961 gaaacaccgc caagacagag gactatcgtt tcccggtttc cgcggccatt gaatcggatt
     1021 accgagagca ggacgctcgc gccgcccaag atcgagtcag actagagcag ctcagatcgc
     1081 ttcgaaaaat gctcaagaaa acgcgacgac ggatagacgc gtggcgggag gaccttgcaa
     1141 aggcgaccag atatcgggac tatgcccgat acggcgagct catgaaggca aatttgagtt
     1201 ccatcacgcc tgcaaaggat caggtcaccc tcgtggatta ctatgacgaa tcgctccccg
     1261 aagtcaccat tcccattgac cggtgcaaat ccgccgtcaa caacatggat gactatttca
     1321 aaaaacaccg caaatatatg gccgctgagc gtgagttagt tccacgcatc gcgcaagcgg
     1381 aaggtgaact caatgcgctg gagcggcaga ttgcgtccat cgagcaaggg acctggacgc
     1441 ctcctgccga aggccgcccg gcccgatcga atccagtggt gaagcgggac acatccgccc
     1501 ccgcacgcgg tcccttccgg cgctttacat ccggcgatgg tcttcccatt ttcgtgggac
     1561 gcaatgcacg ggaaaatgac caactcacat tcggactggc caagaatgac gacttgtggc
     1621 tccatgttcg aggcgcatcc ggatcccatg tggtagtccg gttgagcaaa gggtcggatc
     1681 cgccgccgga aacactgcgg gatgcggcga cgctggcatt actctacagc gacctgaaga
     1741 agagcggaaa aggcgagatc gtctacacaa ggcgcaaatg ggtcaagccc gccaagggtc
     1801 gagccctggg cgcagtcatc gtcacacaag agaaaaccat ccatgtacgg ttggatccga
     1861 cgcgattgga cgccctgaaa gcccgccagg agaggtctct ctcgtaagat ttcatctttc
     1921 gctggccgag catgtcctct tgagctagtc tcagcagaaa cgagtatctc acctaaagag
     1981 ggaggggtcg tatgtcgccc tcgccacctt cagcaaccgc cggcccgacc gtgcttgtcg
     2041 tcgatgatga tgccacggta gtcgcttact gcaagaaaat actccaagaa gccggttgtt
     2101 cgaccctttc tgcgtcggga agctctgaag ccctaaagat ctgtaaacag cacggaggca
     2161 cgattgactt gctgattacc gacctcgtgc tgcctccgcc ggagttctct ttcgcctcat
     2221 cggagaatga atttccccac gttcatgggc acgaactcgc actccgggca ctcaggatga
     2281 ggaaggactt acacgtgatt ctcatgtcgg gaaatccgga tactgatctc gctggatatg
     2341 ggattcggaa agacacactg ccgttcctct cgaagccgtt tgaccctccc gccttcgtct
     2401 cgttggtgaa gcagacatta cacactccac cgcctactcc tgaaatgctg ctcaagcaac
     2461 cctctccagg cacgaagggg gccgacgagt ggtttgactg acgacgaacc gccaccgtcc
     2521 acataaatga aaaggggtgc catgagtcat ggcacccctt cttgtatgcg ttacgagcgg
     2581 tacgacttac cagcggtcgc gcttgccgcc tgcgccacgg cctccaccgc ccccgaatcc
     2641 accacggccg ccgccaccac cagcgcgagg ctcttgcgga cgtgcttcat tcacggttaa
     2701 cgtccggcct cccatatccg agccattgag cgcagaaatc gcggcttgtg cctcggagtc
     2761 cgaggacatc tccacgaatc caaatccccg ggattgtccc gtgaacttgt ccgtaatgat
     2821 ccgcgcagaa gccaccgccc catgcgcgcc aaacagatca ctcaactgct gctcggtcgc
     2881 cgaatagggc aacccaccga cgtaaatctt cgaacccatg tggggttcct cctttgaatg
     2941 tgacattgtc ttgaacacga ggaacgggaa ggagcgggcc gaagacgcga gacatgtggc
     3001 gacttaggtc tgacttccga cgagattccc ggacaaggca acatcgatgt tgcgggccct
     3061 acagctgaac caactccatc gcgaggcccg tacgacggag tatgttaaac cctagcatag
     3121 ggggtggtgg attacaacct gagatgtccc cggaagattc cgaggttgtc tgactcattc
     3181 gcccacccgc accaacgatc cttgtacctt acaataggca aacgtcttat aaaacaaaat
     3241 gattgtcatg accaacaggg cgacattgag cccgccggcc caagccaagt gggcaaaggg
     3301 gaccgtgcct tcacggagca catcacgcat tccttcaaag atatgggcgg ccggattcat
     3361 ccaggccact acctgaagcc aatacggcag cacatcgagc ggatagaaca cgcaggagat
     3421 cggctggaat agaaacacca ggctccaagc caatatttcc gcctcctgcc caaaccgcat
     3481 gataagtgag gtcgtcaaca ccccgatgac ccatcctgta atcaccagat tcacgacaaa
     3541 gggaagcaac cacaaaccca tgatcaacac gttatagcca taaaataacc acgcacagat
     3601 gcccatgacg attgagacgc aggtcacctt aaaaatgctc atggcaatcg tcgcggccag
     3661 gaattcggcc ggcgacaacg gactcgcaaa caggttcatg agattgcgag cccatagttc
     3721 ttcaaggaac gagatcgtaa taccttgctg ggagcggaac agcatgtccc acaggatcaa
     3781 tgcgccgagg aagaaggtaa cgaagcccgg cacttgaggc tgatatttcg ccaggtacat
     3841 ggtgatgaag ccccagatga cgagatccag gaaaggccaa tagaaaatct ccatgatcct
     3901 gggcaggctg cgacgataca ggtagagatg tctcgccaca agtgcaacga cacgatggcg
     3961 tttcacgcgg agcctccggt aaggtccgga ccatgctcat gctcgcaccc accgcatgca
     4021 tggtgcttcc cgagcaactc gtcgatgaga cggcctatgc cttgaagcat cgcagcaccg
     4081 gcacggtgtc cacctggaac gggacgcatc gcaaaccctg caaggatgaa cggtatgacg
     4141 aggagaccac cgacgaacgc catgttggga aggaccgcgc ctgagactga gacgagagcc
     4201 caggcaagca gcaacggtgc gcctccaacg acgagactat tggtgacgac gcgccagctg
     4261 caggtccctc tccacagtcc gatggccccg acggtgagcc agaaaagccc ccatgcggca
     4321 ctcaaggcca gaccacccat cagcacgccg actgcaaaat tgatcacggc actgggcatc
     4381 aagtcttctc tgcgttttaa cgttctctcg ccagcttgag aaacacctct tcaagatcgg
     4441 cttgaccgaa gcgtgcaatg atctctcgag ccgttccctc ggcgacgatc ttgcctcgct
     4501 gaaggaagat gatccggtcc gacatttcct ccatttcacg catgttgtgc gaggtgtaca
     4561 gaatactcag acccgccgac cgacgttctt cttttaagag cgcacggatt ttgtgggcga
     4621 tgtccggatc gaggctggcc gtcggttcat cgagaaaaag aatctttggt tccgtgagta
     4681 gcgccttggc cagcgtcagt cgcgtcatct gaccggaaga aagttttctc gtcactttat
     4741 gacgaaattc ctccatctcc agcttcttga cgatgtcatc cactcgccga tagatttcgg
     4801 agagtccata cagtcgggcc acgacccata agttctcctc cacggtcagt gccatcggca
     4861 tcgagatgta ggtcgacgag aagttgacct gctggagaat ctgttctcga tgggcggcca
     4921 actccatccc gaacacctcg atactccctg ctgttggcgt gatgaggccc agcaacatgt
     4981 ggatcgtggt cgtcttgcct gcaccattcg gccccagcaa tccgagaatc tctccaggct
     5041 caatggaaaa ggagacgtcc tccaccgcgg tgaagtcacc gaattgtttg cgcaagtgac
     5101 tgactcggag cacgggtgtc gtcatggctc tctcggtacc tggttcagaa agacaaaaag
     5161 tcttttattt tgtccggaag gtggcaggtc tcgcattttc ggtccaaaat tctaccctcg
     5221 tgttgggcct tcccgtcatg gcaacggaag caatcgttga gagccctcgt ccggaggtac
     5281 gacccttccg gatactccgg atgccacggc ttgttgtctg tggaaacatg tccacgcggc
     5341 aggacgatgg gatatccttt aatcggctga tcgtgtacga ctcccgaatg acaggtcgtg
     5401 caaccctctt tctggccgcg gacgccgaac gcctccatat gcttgcgatg actcatgacg
     5461 aggcccacat cttttacagg aaccggaaga tcgcggggtg cgacttccga cgtgcgaagg
     5521 atatgttgat gacaactcag gcacacctgt gaatcgacct gagctttcaa attatgtgga
     5581 tcggtcggtg tgccgaacag gtaaatggcg acgtctttcg ttcccgccca cgccttgtcc
     5641 tgcagccagc cctcaatgcc cggcctcaca tggcatgcga cgcacgtgac gtccttgtgt
     5701 gaagacttcg cccagctgtc gtaggacggc ttgatcgtgt ggcaactggc gcaaaatgcc
     5761 ggatggttcg tcaacggaat agcgactgct ccgagcgcca ttgcgccggc gacgattacc
     5821 agcgcgatgg tggctctaga cgtccagctc atgctccgaa ggagcgcgtc gcagcgggaa
     5881 gtgtttcatg atgagtgcgg ctacgccggg cacgtcgttg agatcgaaca acgggacacg
     5941 caagtcgatg ggcttatccg acactacggc cagcaggcct tcggaggaga tgggaatttc
     6001 accgacctga tcacgaatga tgacgatttt aggatatccc tcgtgcttcc atccttcggc
     6061 aataattaaa tcataagacc cgtccaaaaa ccggctccgc acgtcctcca cgttcatctg
     6121 atcagagacg tccgcgaaca tggccatgct gcctttcgac aggatcacga cgctgctcgc
     6181 gccggcttgt ttgtgccgcc agctgtcttt gccctcggta tccaaatcga atccatggcc
     6241 ggcatgcttg acggtcgcga tcttgtatcc cgcgcggacg agctccggaa tcacgcgctc
     6301 gataaaggtc gtcttgccgc tgttcgacct tcccacgaag cagacgatcg ggacgtccat
     6361 cgaaatcctc tcagcagcag gacagccgat gagcgctgtt ttggctcgct tcggcataat
     6421 tcgcccagct ttccccgctg agcagctgaa cggaaaccag atcgcctgga ttaagccgtt
     6481 cgacttcgac gggcacatcg atcaagcagt tggctttgac catcgaggtc aaaatcccgg
     6541 acccttgatc gcccgtcgtt cgtactttga atgcgccgtc ttcacgcgtg agaacgccgc
     6601 gcagaaagtg gcgccgatca ggacgtttgg aaaacttttc ctgaaacagt gcctgcacca
     6661 ccggccggcc gtaactccga tggccgctca ttttgagcat cgcgggccgt accaactgct
     6721 cgaaggtgac catagaggag accggattcc ccggcaaccc gaaggcgagt ttgccttgga
     6781 tttttccaaa ggcgagcggc tgtccgggct taatggccag cttccaaaaa ttcatctctg
     6841 ctcccaggtc gcggaacacc gctttcgtga aatcgtaatc tcccatcgag acgccaccgg
     6901 aaagcacgag gatatcggcg gtcaagccat gcgaaatttt ctcttttagc gcggcgggcg
     6961 tgtcctttgc aattcccagc aggaacggaa ttccacccgc atcctggaca gcggcggcaa
     7021 tgccgtagct gttggagttg atgatctttt cctcgctgaa acgctcgtct aagtcggcca
     7081 gctcatcacc cgtcgaaagg atcgccacgc gtgggcgttg gtgggcgaaa ataaaggatt
     7141 tggcgagaat cgccagcatg ccggcctcac cggggcggat acgagtgccc ttggtaatga
     7201 tccgctcgcc ttttttgaca tcctctccct gggggcggat atttgatcct tttgcttctg
     7261 gtttaaacac gcgcacctga tcgtgcccag gctcggtgtc ctcaaccttc aacaccgtgt
     7321 ccgcgccctt tgggatcggc gccccggtca tgatccggat cgcttggcct ggccctaccg
     7381 ttttggtcgg cattttgcca gcaggaacat cttcaatcac ggtcaaggtc acaggctttt
     7441 gtatggcgtg ttcctggcgg atatcctccc accgcaccgc gaatccatcc atggcgctat
     7501 tatcccaagg cgggttgtcg cgttcgacga tgacgtcctc gcccagcact cgtcccaacg
     7561 catccaacag cgggatcttt tccactccga gtaccggcgt cgcatccaac acgatttttt
     7621 gcgcttgctc gagtggagtt aatcctgaca tgacatcctg gcttttcatc gcttcactta
     7681 gcggtgctgg cgtggggcca cattaaccag cgatccgctc ttcttacaga aggtttccac
     7741 cttgttggcg atctcgtgat aggcctctgc ggtgggcgta tcggaattga acatggcaaa
     7801 cggctctccg gcatccgact gaatgaccac gtcggggtcg agcgggatgc gccccaggaa
     7861 aggcacgccc atgtcttgcg ccgaggcctc gcctccgcct tttcgaaaca cgtcgatgtg
     7921 ctgatggcaa tgaggacatt ccaggccgct catgttctct acgataccga taatcggcac
     7981 ttcgctgtct ttgcaaaatg tcacagattt gcgagagtcc aacagcgcga cttcttgcgg
     8041 ggtggtgatg atgacggccc cgctgacctg gcccagcaaa tctaccgtcg tgaccgactc
     8101 gtttcccgtt ccaggcggca aatcgatcaa caggaaattg agatcctgcc attcgacacc
     8161 ccccaggagt tggttgataa actcatactt gtaggcatcg cgccagataa tgggatcatc
     8221 ggaattctgc aacaggaacg acatcgacgc gatcttcaga ttatacgcct gaaagggaat
     8281 gatccctccc gacgtgctga tcttgagttt ctgaccttcg gcaccgacca tcttggggat
     8341 gttagggcca tgaatgtcca tgtcacagat gccgacatgc caccccttca gcgccaggct
     8401 caccgcaaga ttggtggtgc acgtgctctt gccaaccccg cccttgttgc tcatgacgag
     8461 gaccttgtac tcgatccgct ccatccgctt ggctaccagc cagcggctgt ggccttcctt
     8521 gtctttctga caggattcgt tttcatcgca gatcgcgcac gcccacatat aggtgcaggc
     8581 atcactgccg ccggacgttg gcgttgaaat tacattgagt tcacgaggca tgaggtcaga
     8641 ccctttctcc cggtaaattg ccctgccttg acggcgcgtg caagctcgaa ctgaggtcga
     8701 tccgcactat cgacgccgcc ctcccgccgt gggcacgccg acatactcgc cgtccccggc
     8761 gcccgacatg gcaggcgcca tggctttagg cccgaccggc gacagagcgt tgtccggttt
     8821 ttcctgagcc tttttcttgt tgaagaaccc cgcactgata atgcccacct cataaaaaat
     8881 gtacatgggg agggccatca gacattggtt aaatggatcc ggtgtggggg ttaaaatggc
     8941 ggcgacaatg aaggcgccca gaagcgccca cttgcggtag cgtttgagaa agggcgcatc
     9001 gacccatccc agtttggcca tgagggtaat ggccaacggg acttcgaaga tcaatccgaa
     9061 caccatcaga aaccacaatg cgaatccgac atattgcgcg atcgagagtt gcggaatgaa
     9121 tcccgcgctg accccatagg acacgaggaa gttcaacgca aacggcagca caaagaaaaa
     9181 agaaaacccc acgccggcat aaaacgccac tgtgctcaac gccacgaacg gccctacgaa
     9241 ccggcgctcc tgcgtgtgca atcccggcac gacgaattgc cagacttcca aaaggatgta
     9301 cggagtggcg aggacaatgg caaaaaggcc ggctactttt acgttctgcc aaagagcttc
     9361 cgccggagcg aggaagacga acgggacggt cggcagatcg gtcggcaccc atgaaaggga
     9421 gcctggcacg aacatgttct gaagcggaac acgcagccac ttgacgaggg tatcagcgta
     9481 aaagaacgta ccgacaaaga cgactgccgt ggcgatgacc gcccgcgtga gccggacctg
     9541 gagctcgacc agatgctcca tgaccggcat tttcttgtct tccagcggct tgaagacgga
     9601 atcttgcagc caccggttga agtcgttcag cactgaatcc ccagcagagc cgcaggcgcc
     9661 ggtcgaggga ccggcgcgcc gacgcctccg gattacttcg gattgaccgc cacgaacagg
     9721 ttgttgcctt gccggctgag cagcagaacg gccaactcct cttttttgat cttgtgtgca
     9781 gctttttgat attcgtcgag ggttttgacc acttcgtggt tcacttcctg aatgacatcg
     9841 ccccgctgga ttccagccgc ctcagcagga cctccgggtt cgacagaaga cacgacgacg
     9901 ccggccgtct tggccggaat gttcaactga ctcatcatcg cattgtccaa ggactgcacc
     9961 ctgagggaag ccagcacgtt atctggcggc ttgatcgtct cgccctgttc cttcggaggt
    10021 gccgattcct tcttcgccag tatctcgtcc gatggccgtt cggctacttt gactgagagg
    10081 gtctgctcct tcccttcccg taaaatcttg atctgggcat ccttgccgac aacagtgcga
    10141 gcaaccagat tgcgtaattg actcacgctc tggacttctt tgccattgaa cgagacgacg
    10201 acatcgccac gtttgactcc agcggcgtga gaaggcccgt tctcgttgac gtcgctgatc
    10261 aagacgccct tgcgctgctc cgggagcttg aaagacttcg caagagccgg cgtgatttct
    10321 tggatcgcca cacccatcca cccgcgcacg actttacccg tcttctgcag actgtccaca
    10381 atatccaccg caatgctgct ggggattgcg aatccaatgc cttcggatcc tccggtcctt
    10441 gagaaaatgg ccgtgttgat gccgatcagg tctccgttca tgttgaccaa cgctccgccc
    10501 gaattacccg gattgatcgc cgcatcagtt tggatgaaat cttcgtaatc tgcgatcccg
    10561 acgttccccc gtccaagcgc actgataatg cccaaggtga ccgtcgagct caatccgaac
    10621 ggactgccaa tcgcgagcac gagatcgccg acctggagtt tttcgtattc cgcccatttc
    10681 accgatggaa ggtccttgac gtcgatcttg accactgcca gatccgtctt gggatccgtc
    10741 ccgatgattt tggccggaaa ctctcgacgg tcgctcaatg tcaccgtaat ttgagtggcg
    10801 ccctccacga cgtgattgtt ggtgacgata tacccgttgg gatccatgat gacacccgat
    10861 ccggcgcttt gatccggacg cccatgagga ccggggggcg gcatcggcgg aggagtcggg
    10921 agttcgccac ctggctcatc aggggggcca ccgcccgggg ggccaccgcc cggaggtcca
    10981 ccgaatgggc ctggaggaag cggtcgtcgg cctcgtccgc cttcgcttcc gcctgtcacg
    11041 gcgatgttga cgacggcggg tgtcactttt ttcacaattt ccgaaaaccc ctgcgccatg
    11101 gaaggaggca ctcccgccgc cgtggccggc gagtcgcctg ataaggggct cagtgtacaa
    11161 gcaagagcga agacgcacac acaactggcg aatgctacca cacgaccgtt ctcactccac
    11221 gtagccatga ccgtgtcctc cgacgggtta tacgatttcg ttaatatatg gggatgaaca
    11281 gttcattgta cactatccct tccaaggcct tcaaagacta atcgtcccct tcgggccgga
    11341 cacacccgaa agggacgatc gatgaggagc agcctggcga tacttacgag gcggttctcc
    11401 gctccgccgc atcagcctcc aattgtccaa cccaggagtt ttgcagtttc agcgccagcc
    11461 gtttctctgc ctgatcaaac gacaccaccc gcagggtgtt cggcgcacct tcgtaggtgg
    11521 gcggccaaat ccggcgctca gggtcttggg gacgcaggta gatgaccggg taccattgac
    11581 tgattcccgg ggccgacggc cgatccaagg tagcccagcc gcgtccctcg gcctgcatca
    11641 gcgtcacgtt gttcttgaga tccagcaggg cgaactccag gagagaccaa ttgtcccgac
    11701 ggaagcctgg ctgagcatga gtcgtccaac caagaaacaa tgtcaccgga tattcctgtt
    11761 cggtgctgga cagcaccaca acggccaagt actccagccc gtgcagcctg cctaactccg
    11821 caaattgcgt ccaatccccg ttcggctgtg gacggatctg atcggccggt agaacctggg
    11881 tgatggtgac aggtatcgtg cgagcgatgt cctgcttcaa cgtttcgctc aggcggacca
    11941 gtgcctcgtc gggaagattc ggggccgccc ctggctcggc agtgtcagac accaaaagga
    12001 gcccggcctt gagcggtcgc tgttcgatcc gggaaaaggc ctcgtcgttg gccctatggt
    12061 cggaggacac gtagtcgcca agcctgctcg ggggagtagc cgaagcacag gcggccaaca
    12121 gaacggcaac cagcaggaaa ttggacacgt ccacgattct acgcatgcga ccctcccacg
    12181 ttagatggac gctgcaactg tgtcgcctga tgtgatgata cgttcgctcc cttatgccgg
    12241 atctgtcaag cccacgattc cccttgtatt ttctccccta cctcggctag ggtaccgcga
    12301 aagagatgga ctcgcagcgt ttctccgtcc ggaaccgcag gagtcccatg tttggtcacc
    12361 actccacgga ctttcctgaa ctatcgatcg tcattccagc tcataacgag gctggtcgta
    12421 tcctccctta cctccttcag atcacccaat attttcgaca gcataaccgg acgtatgagc
    12481 tgttggtcgt cgatgacggc agtaccgatg cgaccgcatc ggttgtcgaa gatgtcagtc
    12541 gcgccaatcc ggccgtccgg ttgcttcgac tggcacgctg ccggggcaag ggggcggcgg
    12601 tgcgccacgg tatgcaatca gcagtgggtt cgctgcaatt attcgccgac gctgacgggg
    12661 caacgcctat agaggagttc gctcgcctcg aaagagccct cgccgatgat gtggacatgg
    12721 cgataggctc tcgagcgctg gcctcgcggc tccccgagtt tgcggtccag gctcggttcc
    12781 atcgaacact gtttggcatg ctgttcaatg ccgcggtgcg tcgcagcggc attcgaggga
    12841 tcgcagacac gcagtgtggc ttcaagctgt ttcgccgcgt tatcgcacaa gacttatttg
    12901 cccacgcctc gatcgatggt tttggattcg atctcgaagt gttatacgta gctcaacaac
    12961 ggggttatcg catcgccgag gtgccggtga attggtctga tcagccaggt tcaaaggttc
    13021 gtgtgctcaa ggacgggatc gccatgctgc gcgaactggc cacgatcaaa cgcaatgatc
    13081 attgcgggcg ttacagcctc cagccgaatt cgaccggatg gggaaacgtc agcgcggatc
    13141 gcctgaggat tccagttcga taggcaacag ccgataaatc gcaacctttt cgtctccata
    13201 gagacgcgtc caaccatgcc cctgggctag tgctccatcc aatggcgtct gctttctcac
    13261 catagcccag tcgatcgaat attttgtcag tacggtgaga tccggatccg ctcccgtcaa
    13321 tgccatgtag tccggaaata tacggcgctc tccgcagcgc caggcaggca tccgaccgtc
    13381 aataaagacc cgggtctcag gtaaccacca gagcaaaaat cctccgtacg cgtaatcatt
    13441 gtacagacgc ctgcctatca gatcccggtt catccgtatc cattcaactg cttcgatagg
    13501 atacgaagtc cttcggaaat actcggccgg cctggtaccc gaaaagacga cttgttgcag
    13561 atgctctgcg cctaaccaga agagaacggc gcccattgcc accgccgcgg ccaggctgcc
    13621 ctgccttgcg gtcagacgac gccacagcca ccgacgctcg agtgtgtgga atccttgggc
    13681 caggacgtcg gcgcaagtcg agaggctata aatgagaaaa aaaggaatat ttcgcatgtg
    13741 ccgcagcgag agcgcgagga acacgcctcc aatgacccaa cggaccggtt cagttcgccg
    13801 gtaccaaagc agcatgaacg acgtgagtcc tgcaagatag agcgcatacc tgcggccggc
    13861 gacaccgctc agcgacagag gctgccactc ctgcagcgtc tcgagcatga accgatccga
    13921 caacgaatcc acaatctcag cgtgcaagcg ccagccgtac gggttgatca acgtaaggcc
    13981 gacggagaga atcgtgagca aggtgaggcg cttgagatca tcccacgata gcagcggctc
    14041 atcatacttc cgcgcggaga cgatacgtcg ttcggtcaac catctgagga atgcggtcgt
    14101 gccgataatc agtcccaaga ccatcagccc agccagaaat ccgccgtgga gattcgccca
    14161 gccgaaaaac aacggcggaa tccaccatcg ggcagaagag acgccgtcct gccaacgttt
    14221 gagcaggaac aagagcaccg ccaacccgag cagcgtcacc agttgcgtcc tcgctccgag
    14281 atagggcaat gccacccaga gagagatggc acaggcgagc cgacggaaga caaccccggc
    14341 acgagcggtt gcgctgccta tcagccatgc gccggtcgtg acgcatgcaa acaacaggat
    14401 gatgccaagg ccacccccaa acgaatagac ggccccaagg accacgtcgg ttaaccaggc
    14461 atgttcgacc catgcccaat ccggcatcgt atgagaatag gggtcgctgt gaggcaacaa
    14521 gaacccgttg tgcagaaggt ccaaaccggt ccggagatgc cagccgaaat cgggctccgt
    14581 cagcggttgc agggcaaaat tcacgatcac catgaggagg aggaaattga gcaccggggt
    14641 atcgagggac tgacgaacag cggctgcagt ttgagggtcg tcatgagcag cgtatccgtg
    14701 attcatcatg actggctcat cgagatcctc accgctcagt cctgctcatt ggccgcctcg
    14761 ccaataaatt ctccataacc agccactcga cttctgtgcg gagaaagcag gtcaccgcgt
    14821 catcgggggt acacacgatg ggctcgttca cgttgaacga cgtattcaac aggaccggca
    14881 ctccggtctt gtctgaaaag gcttcgagca gccggcgata ccggggattg gagtggccat
    14941 cgacggtgtg cacccgtgcg gagccatcga catggacgac tgccgggatg actcctttca
    15001 ttgcggaacg gacaggggcg gtgcacaaca taaacgggga aggcccgggc aagtcaaaaa
    15061 attcggcggc ccgttcttcc agcacggaag gcgcaaaggg ccgaaacggt tcgcggaact
    15121 tcactttgag attaatcagc gcccgcatgt cttcgcggcg aggatccgct aagaggcttc
    15181 tgtaccccag cgcacgtggt ccccattcca tccgcccttg aaaccaaaag actaaccgcc
    15241 cggcggccaa ctctccggcc accttttcac acagcgagga atcagcgagc cgttcaacca
    15301 tgagcccggc gccttcaagt gcggcccggc aagcctcttc cgaaaatttc ggtccccacg
    15361 aggctgcacg catgaccgtc cgatcaacct cccgcccctt ccgatgtgtg agccacaagg
    15421 ccgcgccaag cgccgcgccc gaatcgccag ccgccggagg aataaacaag tcctggaatc
    15481 cggcctctcg ccaaactcga ctattggcga cgcagttgta ggccaccccg ccggccatgc
    15541 atagctgctt ttccttcgtc aaaactttca agtgtcgagc cacgtaaagc agcacttcct
    15601 ccaacaccag ctgcgcactg gcagcgaagt cttgatgacg ctgtgtaatc gcttcgtccg
    15661 gccgtcgcgc cgcaccaaag aggcgcaaaa attccgatga aaagacccgc cggcgcgcaa
    15721 gatgaaaatc cagcagacgc gtgttcagac aaaactctcc gtccggcaac acgcgcagga
    15781 tcttttctcg caggaccggg gcgacgcgtg gctccccata ggccgccaat cccatcacga
    15841 tgtattcgtc gaagtccggt ttgaacccga gataggccgt gaccgcagca taaaattggc
    15901 ccagcgagtg aggaagggga atgcgattga ggacccgaat atgatgacgg tccgcttgtc
    15961 cgagcatcgt ggtgtgcgat tcagaggctc catcaacgac gaggactgca gcacgctcaa
    16021 acggcgatac cagcatggcg ctggccgcgt gacagagatg atgatccagg aatacgggag
    16081 ccggacaggg agtgccgtcg acgatgcggg agagggagga ccgtaaaaac gccaactctc
    16141 tccactcatg cgtcagacgc tccaggctcc tggtgccttt cgccatgaag agctgcggag
    16201 accgaagcat ggagccgatg gcaagcgcag cacgtcggcc gacctgccaa tacttccatg
    16261 ggacggcgac ggcatcgacg tccgcaagac gaattccggc ggtttctagg cagaatcgaa
    16321 ctgcatggac tggtaaagcg gtgacatgtt tgatccgaac aaaccgttct tcttccgcag
    16381 ctgcaacgat ttgaccgttt tcgaccaagg cagccgccgc atcgcgcatg ttcgataacc
    16441 ccaggatgcg catggcgcgg tagaataaag cagccggctg ggcgtgacaa gttgctttca
    16501 gacattgatt ccggtacggt gagccacgtc catgctcacg agtctgcgtc tttatctgga
    16561 caggttcact ggtagatttc tctgtacgag tacccaccgc atcgctctca tcttgatcct
    16621 gacggtatcg ggatgcacga agcaggagct tcccgacctg ggacctcgcc ttccacagac
    16681 tgcatccatg gaattctcgc catcgttgct cgagtccaag gccgaatata cggacaattg
    16741 cggccacatt cagatagtgg atatcggcag cacgctccag gacgtcctcc tggaagccgc
    16801 caatcgcacg ttcacgtccg tggtccggcc cggctccggc gccaagcctg acgttgtcgt
    16861 ccgcgtgaat ctcattcaat ccacatttaa actgcggatg gacggtgtgt atgatcgggc
    16921 tgagaccgag attcagcttg gcggtctcgt gtcgtatgtc gatcaggccg gcacggcgct
    16981 tggagaacag gaggtacaag tcgtccagaa ggggcgcgtc cggattatgc ccgtgcaaaa
    17041 aaactgcgac tacctcttgg acagctttat ccaggacgcc acccacgact ttgcccaaaa
    17101 atttgcagtc acgaccaggg cgaaactcgg tgctcccggc gcccccctcg ccgccgccgc
    17161 tgctccagcc gccatacccg ttccagcccc gacacggcct gtcccgtcgg gcgcagcacc
    17221 tctttcgttc aaggccacgg tgttggacga caacagcaat ctgatattcg aaggcggaga
    17281 gcggatacgc gttcgtgtcg atgtggtgaa tacgggcgcg caggatctgc agaacgtcac
    17341 ggcaaacctc agcggatcgc ctcaagtgct cgcccaattt ccgacgacga ccctctcagc
    17401 cggccggctg caacccgggc aatcccggtc cctcgaattt gtcgcaacgc ttcctcagac
    17461 cgtgcaacaa cagaaagccg aactccacgt ggctgtgttc gaccctgctg cggggtcatc
    17521 accgtcgcag gccctttcac tttcgatcca gccaaccggc acgaacactg atgacgtcga
    17581 tcaagtgccc gccatgcccg tggggttcaa gcggcctcac acctacctga tctcgatcgg
    17641 catcggttca taccgcgatg tccagggagg cgccagaaag tatgcggcct ccgatgcgga
    17701 actgctgacg acctacttcc agtcattggg cggattgccg gcgtcgaacg tgcgtctgct
    17761 tcaggattgg aaagccctca agccggacat tgatgaagcg ctattggact ggctgcctgg
    17821 gcacatgaac aaggacgcgg tggttatcgt atattttgcc ggtctggcaa ccgttgcgcc
    17881 caatggagac gtctatctcg ttccctatga cggcaatacc accaacgcgt cgcgctcata
    17941 tccgctcaag gaccttgacg ccgctcttgg ccgtttgaag gccaaacaga cactgctctt
    18001 ctttgacggt tcgctgacca ggctgggagc agacagccga tcaaaaactg tcgtccccca
    18061 atggagtcca tccggaagct cgacggtcca tgttgtcgcc atgaacggca tcggcaaagc
    18121 cgtcgaagat gaagggcatc gccacggtct gtttacctac tacctgttgc gtgcgcttcg
    18181 cggcgaatca gacgtgaacc gggatgggga agtgaccctc ggtgaagcga ttgcctatgt
    18241 gggtcagaag gtcagttggg cctcgaaaac gcactatggg caagaacagc gtccggcggt
    18301 gtttccgtca ctgaagacga ccgatcccat cagcggtcta gcccttgcca aactggtcgc
    18361 catcaaagct gctgaacaac cctgacaaaa caaaacgggg tggaggaccg gtcctccacc
    18421 ccgttttcgt gcttcggcgg ctgtcttatt cgtcgccttt gcagaaactc cgctcgtaat
    18481 tgatgatcgt ccaggcttct tcttcggtaa tggcagctgg gatcagggag accattcccg
    18541 ttccggcact gccgttcttg atgacccaga agagttcgcc gtctttccgc ttcttatgga
    18601 atttgcagtt cgtgaagttg cgggggctag gattgaggat cgcaccggcg ggaccgtccc
    18661 ctttcccttc tttgccatga caattgaagc aggtgccctt tccttcaaac aacgcctttc
    18721 ccttcgcgac gctttccggt gtgttcgcca ccggattttt cagcgccttg gcgtccgcga
    18781 tctgatccgg tgggacgcga ggcttcagcg gatctttctc ttccgctccc accacagtgg
    18841 ccgagagcaa cataactgcc gcagcgactc ccaaaaactt agaaaaatac cccatcagac
    18901 ttcctccttg ttgttaaacc accgcgcatc acagggccga ggcccgctgc ttgggttgca
    18961 tgaaggtaag aaagcgtcgg gactatagca acgccgtttt gacattgtca agcaaacgac
    19021 tcaggcatgc caacggccat cgtccccgac gctgcccaca cctcactcgt cgcagatgat
    19081 caaacgtcat gccacttcga ccaatttgtc tggttcgaaa tttcgcttag ttagcactgg
    19141 ctgagggagt cactcgcgat gacgcgagct gtcgagcata ccaatacgcc acagagggca
    19201 ccgcaccgcc acagggaaga cccctcaggt caaaggcgtt cgacgggaag aggaggcgtt
    19261 aagggagcac acaacgcgtt gcaaggttct gctaacgctt taagagaatg tcacgggcaa
    19321 ctttcccgct gggaccaaag ggcttttgag gctttccatt gagctcggcc ttgacgccgc
    19381 ctgcattgcc aagcgtgagc gtgaactggt cctgtccttt ccacttggcc ttttccccgg
    19441 gacgcagcag agcctcctgg ggacttcctc cgtctatctg aatgaccacc cagctgagtt
    19501 ccgtggcttc caggtccagg accaatggct catccgtatt cgcctgaccc tgcagcgtga
    19561 gtgcactaag gggcccctcc gtagccggag ggggcggaac aggcgcagcc acggtggcat
    19621 gaggctgaac tgggggcgga ggcggagccg gtgcagcggc acgactggag ctcgcatcgg
    19681 cacgagcgag aggcggtgga gtcggaagct cggatggctt ccccttcacc gcgccgggtt
    19741 gagcctcaac ctcaggacga gcgggcaaat cactcttcgc aacgtctctg ccggactcgg
    19801 ggggagccgc gcgctttccc gccatcgcgg atggttcgct ctgagatgtt cgtctgacca
    19861 aaagcgatga ttgctcgcga ctcagtagaa tcacgagcgt aagaatggca agcccgatgg
    19921 cgatggcgac cgcctttcga ttcgcacggc gctttcgttc ctcttccgcc tgccttactt
    19981 taagccgttc gcgttcatcc tgcttttcat agaaggcccc cgccgattgg ataaaccgat
    20041 gaatggcatc ctcttcatcc aaccccagcg aacgtgcata ggagcgaaca aaccctcggg
    20101 caaagacctg gtcgggcaat ttggcaaaat tgccttcttc caatgccttc acgaaatccg
    20161 tgcggattcg tgtcttcgag gctacttcct ctagcgtcag ccctttggtc tctctcacct
    20221 gcttgaaaaa atcgccgatc gaatccatat ggctctcttt acggcttcgg ccgtgccaat
    20281 agttccttgg cggctgctgc atagctgccg ttcttgtcca atgtcgtgac tttggtcagc
    20341 gcgtctttcg ccctgcgctc gaacccgagt ttgtaataca cccgtcccaa ttccagttgc
    20401 agcatcgcgg gcgggacatt cggcgggttg acgaccaagg catcctccag cgcttccatg
    20461 gcgccttgat agtcaccttc atgtgccagc gccttgccga gttgatagcg ggccagatcg
    20521 ggagtgctat agaggggatt gctcaaggcg cgtcgaaact cagcgatcgc ctcatcccaa
    20581 cgatcctggc tcgccagcac ttggccgaga taggtgtggg cttccgaata attctcatca
    20641 atcttaatgg cttcccgaaa ctcgctttca gcctgtgaca gcttgccttg atacaccaga
    20701 atatgaccga ggccgtaccg tgcttctttg ttcctggggt cgagctgcac cgccttttga
    20761 aacgagacgt aggatttttg acggtcgtgt tccaggctcg ccatcccttc ctgataatag
    20821 cccttcgact tttgcttcat ctcatcggac gtcccgcaag ctccgacgag tacaaccgcc
    20881 agcatgagga gtgccacaaa accggcatac gattgcggcg gcataggtaa ccggttcacg
    20941 gtaggtcctc aaaatgggtc agacgcttga atgcccggaa gcgatcctgg atctctttgt
    21001 aatccaggac tcgcaagcgg tcaagactaa aggcttctac ggtaaatgag gccatcacgc
    21061 ttccaaaaat gatggcctgc ttaaacgatt caacggatcg gtttccggtg gcggccagat
    21121 agcccaggaa tccgcccgca aaggtgtccc ctgcgcccgt cggatcacgg acgtcctcta
    21181 acggaaacgc gggagccccg aacacctggt tctcattgaa catcaacacc ccgtattcgc
    21241 ctcgcttgat gatcagatgc ttgggccctc gtgccaggac gctcgcggcc accttgacga
    21301 ggttggagtc cccgcccagg gcccgtgctt ccccgtcgtt gatgataaga atgtcgactt
    21361 tctcgagcac ccgccacagg gcgtcccgct tgccgctgat ccagaagttc atcgtatcgc
    21421 aggccactaa gggcggccgc ggcagtttgt tcagcacatc cagttgcaac tccgggtcga
    21481 tattgccgag aaacaagacg tcgggagagc gataggattc cgggattttt ggacggaagg
    21541 tctcaaacac gttcaacttc gtatcgagcg tgtgggcttc gttgagctgg tgggtgtatt
    21601 ctcccctcca cctaaatgtc gcaccggaac gccgttcgag tccggcaaga tcaatgtttc
    21661 ggcttttgag gaacgcgagg tgctgagagg gaaagtcctc gcctaccacg gcaatgaggt
    21721 caaccgcagt gaaatagctc gcggacgtgg agaaatatgt ggccgatccg ccgagcacct
    21781 ccgtggcctc accgaacggt gttttcacgg tgtccaatgc gaccgacccg acgaccaaaa
    21841 gtttgcccat gattcaccgt tttcctttca caggtgagag gacccgttca atcaagagcg
    21901 tcaatctccg gcggagggcg aggggaatct tgtccggagc ggtgacaatc gcatgctgca
    21961 aggcacgctg acattcgcag ctcgttgctg acgccgccga tttcaaggct gccgccagca
    22021 gccgtttagc cagcgcaaca ttttgacata aggtggcaag aatggcctcc acggtgacgc
    22081 actcctcggt ctcatgccag caatcatagt cagtcaccag tgccaccgtg gcgtaacaga
    22141 gttcggcttc gcgggcgagc ttggcttcgg gcagattcgt catgccgatc acgctcacgc
    22201 cccactggcg atataactgt gattccgctt ttgtcgaaaa ctgcgggcct tctatgcaga
    22261 cgtaggtgcc gccctggtgc accgtggctc cgaccgaccg gccggccccg agaagcgatg
    22321 cgctgagtga ggcgcacacc ggctcgccga aggccacatg cgcaaccagg cctccgtcaa
    22381 aaaatgtcga gagtcgtcgc ttcgtcaaat cgataaactg attcgggagg accacgtcgc
    22441 ccggctttat cgattccttc atgctgccca ctgcgctcac ggaaataact cgtcggacgc
    22501 cgatcgactt caaggcataa atgttggccc gataattgat ttcggaggga ttatatcggt
    22561 ggccgacgcc atgccgcgac aagaacgcca cccgaactcc gtcaagctcg ccgagcacga
    22621 tggcactcga aggcgatccg aatggtgttc gcacgtctac gcgtttgacc tgatgcagcc
    22681 cttccatgtc gtataagccg cttcccccga tgatgccgat gtccgcacga gacgcccgcg
    22741 tgagcttggt cacgatgccg cctttcccta ccaagaatga ggaactagtg tgtcaatatc
    22801 tccgtccagc ccttcgagaa aaggatggat caagtccacc actcggctca ccgtctgttc
    22861 ggctggctcg gattcttcta tcatgagcca ggtcagctcc ggttcccggc ggaaccaggt
    22921 catctgccgc ttggcaaacc gcctggtgtc gcgcttgaac tcgttcacca tttcattcca
    22981 gttacactca ccggcaagat agctggccac ctgacgatat cccagccctt tcatcgccgg
    23041 ggagtcccgc ctgtatcccc tccgaagcaa tgtctgtgtt tcgtctatca aaccgtggct
    23101 gagttgccaa tcaatgcgct cctcgatgcg acgatagagg tgatcgcgct cccggttcaa
    23161 cccgatgacc agagcagaaa acggtcgctc gcgaaatcca tgcttcgatt ggaaggacga
    23221 cagaggcgag ccggattgga gatacacttc cagggcccgc aggatctttg gggtgtcccg
    23281 agggtgcaac ttggccgctg tcagcgggtc gaccagggcc aagcgggcat acagctgctc
    23341 acccccctgt tcatacgcct cgtggcggag ccgcgcccgc acacaggggt cgctcggcgg
    23401 cgcttcacaa agcccttgca agagtgtccg cacatagaga ccggtgccgc cgaccagcaa
    23461 gggaagcatc cgccgtcgat aacactcgtc gattgcggca gcagcatggc gccggaagag
    23521 accggcattg aagggttcgt cgggatcgac aagatcgatg agatgatgga cgatcccttc
    23581 gcgggccagg agcgtgggct tgtcagtgcc cacatccatt ccgcgataga cctggcgtga
    23641 atccgccgtc aggatttcgc ttcccagata ttttgccact tcgacggcaa tccggctctt
    23701 gccgacggcg gtcggtccta gaatgaccac gaggggcttc cggcgcagtt gtcgctcgga
    23761 aagtgagggc atggtgagat gagagcaggt cgtcacgacc atccggtccg gccgaagagc
    23821 gtgtccagtt cgtccgccga gaggcggaag gccgtgcgcc tgccatgcgg gcacgtggac
    23881 atcacccctt cgtctaccca gtcgcgcacc aactgttcga tttcgggaag cgccatgggc
    23941 cgtccggctt ggacggcact cttacaggct agcgtcgcga gaaccgcctg tacacgtgcg
    24001 tccacagagg aaacactcgc ccattcagcc aagtcctcga ggacattgcg tacgactgaa
    24061 gcgccgtcga cgggcccgac gccgacaggc accgctctca ggacaacggt attggtaccg
    24121 aacggctcaa gaaccagacc caggagttca agatcttttg catatttctg catcagcgta
    24181 tattgggcaa cggtcaaatc aatcggctct ggtatcagca gaggctggat ggccatgtcc
    24241 cgcgccatcc atcgccgcca caagcgttga aacagcactc gttcatgagc cgtgtgctgg
    24301 tcaatcacgt gcaattcgag cccgacctgc gcgatcagat aggtctgatg catctgtccc
    24361 aagggcgtaa tcgtcacatc cttcgacgcc gggtgggcgc ctgtacgctc cgccgcaaac
    24421 agtgaatccg gcctagacgc caggcgctgg tccgcttgct ccgcgggtcg ccatccggac
    24481 gatgcgctgg aagagcttcc atccgtaaag gacgccatcg cagtggcagt cgctgggtcg
    24541 tcggtcaaac gaaggaccgc tttgcgctcc actccactga acgcatgtcg cacgctctgg
    24601 cgtacgaacc ggtgtatgcc ttcctgatct gcgaaccgga cttcgcgctt tgcaggatgg
    24661 acgttcacgt cgatgcgctc gggatcgaga tcaaggaaca gcacgtaggt cggatggtgg
    24721 ccttttgcca gcaacgagcc atagccttcg ctgatcgcat ggaacactgc ggcattgcgg
    24781 acgggacgtc ggttcacgaa aagatcttgc ggcgccctcg aagagcgcgc gtgaacagga
    24841 tcgatcatga cgccgctgat cgtgcatccg gcgatgcggc cccgcacctc tactgtccgc
    24901 tcgagaaacg gctgtcgata cacctgcaga atccgatcgc gatcggaagc aacggccgca
    24961 tgattcagga cttcctgccc gttgtgggtc agtcgaaaat ggacgaacgg ccaggcgagg
    25021 gccgcttgct ggacgacggt gctgatatgt gagaattcgg ttggaatgga cttaagaaat
    25081 ttttttctgg ccggctggtt ataaaagagg tccgagactt cgatcttggt tccgatcacc
    25141 gcgggagcat caacgagttg ttgaggcacc ccgccgatca atgacaactg cgccccgacg
    25201 aggtcctcgt tcgtggccgt ggccaccagc actttggaaa cagaggcgat gctgggcagc
    25261 gcttctcccc tgaaccccat cgtgccgatg gacgtgagct ccgcatcgga ggtcagcttg
    25321 ctcgtggcat gacgctcaaa ggcgagggga atgtcggatc gtgtgatgcc ctggccatcg
    25381 tcggtcacac ggatgagtga agtaccccca tccctgatgt cgacggtgat gtgccggctg
    25441 ccggcatcga gactgttttc gatgagttct ttgacgaccg cggcaggccg ctcgattacc
    25501 tctccggctg caatccggct gacaacatcg gccggaagca ctcggatcgt cggagaacag
    25561 cgtgcggcgg gcaccagctt agactcccaa cacccatccc atcatcgaat gtctgccagc
    25621 ttgtttttgg caagcttggc ttcgtccgag gtgggaaatt cctcaatgac ccgcttcaga
    25681 tttttcttcg acttcacgag atcgccggtc tccgccgcgg ccagccctaa cttgtagagg
    25741 gcggccggta ccttttcatt gcccgggaat tcggttgaca catattcgaa cgcctgcatc
    25801 gcacgtgcat aatccttctg ctggtagtag gactctccca gccaatagtg cgcattcggt
    25861 gtcagcgagg tccccgggaa gtctttgacg aaacgctgaa aacccgagac ggccagatcg
    25921 tatttcccgt tcaggtaatc gttatacgac aggttgaacg ctgaagtggg agtaatagcc
    25981 ggaacgttgg ggacgatgga tgacccttct gatccagccc gttgaggctt gatcgttctg
    26041 gcgggttcgg tcggagcggc ctcagattta agtgccgggg aagccgttgc tccctcgtcc
    26101 aacttggcta cacgggcctc cagtttctgc aaccgtgcat tgaaatcctc aagcctggtt
    26161 ttcggcgcct ccacatcttt cactcgttcc atcgattcca tgcggcgctg cagcgcgtct
    26221 aaccgctttt gatcctggtc ttgggacttg gctacagtcg ccaaatggtc gcgcaactcc
    26281 acaaagtccg cgtgcttagc gcatcccgaa accgtcaagg ccgtcacggt tacgcatccc
    26341 accgccaaca tcatcatgcc gtcgtgcacc gttatcgtag ttcctttaat tggttcagtt
    26401 tgtcataggc tttgcccgct tcgggactct tgggatacag tgtcacgacc tgcttgaaag
    26461 ccgaggaggc ccgcttcttg tctttcagcg caagataggc aaagcctttt ttgaggatcg
    26521 ccgccggaac tttctcactc tgcggatagt ccatttcgac ccggtcgtag gaatcgatcg
    26581 cctgcttata atctcgctta ccgtaatgcg attcacctag ccaatagcgg gcattggggg
    26641 ccaactctga gttcggatac tcggtcaaga atgacgagaa cccctcgcga gatccttcca
    26701 agttgccttc cttaaaaaag gccaacacgc gttcgtattc ggccctgtct gatcgttcgg
    26761 gaggacgtac aggagcagca agggcaacgg gttcagccgg ttcatccgcc gcaaccgtcc
    26821 ggtctgcgac atgattcacc gtctccggct cctggcgtgc cgcggcaacc ggttcagctt
    26881 cgtgatgaga gacgccttca gccgggccaa ccgcagagga ccgctgcatg gactttgttc
    26941 ccgctgactg ctgacgctgg ccggctttgg gactcgttcc gcgcgtatga acttcctgag
    27001 tcacatgatc gagcgacttg gtgagtccgt cgatccgccg gtcttgctcg gccacccgcc
    27061 cgtcgagctt cgtgacggtc ttgttgatct cgcccatctg ttcatcgacg cgggcagcaa
    27121 gtttctgagc gaccgcattg acgctcttat tcacctcacc tagatgtccc gacatcgacc
    27181 tcgtcgtctc attgatgtga gcggtggatg ccttggtgtc ggcttcgacc ttggccgcca
    27241 aggtcttgct ggcctgttcc tcctgagcga tccgatcccc caacgaggac agagactctc
    27301 gaaatcccgc taaggcctga ttgaactgcg caaatttcgc cgacagttgc tcggtacggg
    27361 cctgattgtc cgccaacgct ttgcgctgtt cctccaattt cacatccaag ctcttggcga
    27421 acgccgtatt cgttttttgg acggcatcaa tgatgtcttt acgcagcact tccatctggt
    27481 gattgacatc atcgagtcgc gtattgacgt ccaccctgag ctgatcgcgg tccgccttgt
    27541 tcttgaggtc ttgcgcattg aggctttcgc gcacctgcgc ataacgattg gcggtgtcag
    27601 catccagttt tccggacaac tgttcgagct tcctggtctg ttgctcgaga acggcggatc
    27661 gctgcttcag gtcatcctgt gcccgttgta tttcctgagc ctgatggtgt gcccgttcga
    27721 gttcgccccg aagtttcggc agctcctggc tcttcagctc ctcgagttct tgccgctgtt
    27781 gcgcgccccg ctgcgccaag gcgtcgcttg attccttgaa ccggcgttcg gcgcttttca
    27841 aatcggcttg ctgggccacg cagccatgca agagcgcggc ggaaaacaca cagaccacag
    27901 tcccgacact tgcgacattc cacccgatgt tcatgtgtta cctacgtccg cctcgttggt
    27961 tacttgcctg acttaacaac cagatgtccc cgccggttct gctgatagca ggattccgca
    28021 tgctctgcgc aggacgggcg ctcttttcca taggatacga ctgacaaccg gttcgcgctg
    28081 atgcccaact ccaccagaaa atttcgcacg gccttcgcgc gcttctcgcc caacaccaga
    28141 ttgtacgccg aggtcccccg ctcgtcgcaa tggccttcca ctttgaccat ggccactggg
    28201 ttggatttca tccaatccgc atcgcgagtc aacgcttgcc gctggtcttc ggaaatcgtc
    28261 cagctatcat agccaaagta gacgtcccgt aacccagcgc tggctgaggc ggcttgctca
    28321 gcccgcatct cgtccaactg acggcttgcg gcatcggagg gatcagcttt ggccaacaac
    28381 gatccgccca tgcctgcgga ggcaccggcc ggactggcgc caaacccgct ggccacatgt
    28441 tcttccgagg gatttttatc caagccccgg agacccgacc ctcctgactg catgctggtg
    28501 tcggggaaat tcccgactcc gcttttcgag ccctcggtgc tgccgctctt cgcagcactc
    28561 ccttggtggg acgattgggt gtcgccgccc gattgcaccg acttcttcga acaacccgaa
    28621 acgcccatca gggcgacgct ggcaacaagt gccatggcga ttttgaaaca tgttgtgccc
    28681 atgcgcgtgt ctcctctctc tacgttagtc atatatgtca ccggttgcct ttgtcactcg
    28741 gacgtcggtt aggatgccgg agaccaactt ggcgcactgt tgtggcttcc ctcaaatgtc
    28801 aagcgttcaa ggcccttccc gtcagcatcg atcatataga tgtggctctt gccgtcagcg
    28861 gtagaactaa aggccaggtg acgcccgtcc ggagaccagg agggtgagtc atcaatgcca
    28921 cctcctgtag ttaattgcat acgcttttgt ccgtcagggc taatgacaca caacttatac
    28981 tccttacggg gtgtccggca gacgtaggcg atccaattcc ctcttggaga ccaggcagga
    29041 gcggcgttgt agtcgccctc gaacgtcaaa cggcgcacgt ttgagccatc cgagctcatt
    29101 aggaagactt ggggaccacc cccgcggtcg gatacaaaag caagttctct accagtcgga
    29161 ctccaggtgg gagagaggtc gccggccgga ttggttgtga gacgctgtac ggccttcgtc
    29221 ctggtgtcta accgatacaa ctcggaattt ccttcgtggc tggaagcaaa agccaggaaa
    29281 ttcccatccg gcgaaagggc gggagtgatg ttcaaccccc catacgagac cagagtccaa
    29341 cgcttgcccg tggccagttc gatcatatcg atatcttggg tattccgatc gcggtaggcg
    29401 gtaaagacca agaaccgccg atccggagac cagcggggca tcagattgag gaacccatcc
    29461 gcggtcagct gccgcgcatc atacccgtcg taatccatga cgaacagctc gcgagccgcc
    29521 ccgtgggccg acacatacgc aatcttagtt ctggctatgc ccggttcccc cgtgtagcga
    29581 aacaccagtt cgtcggcaaa tcgatgggcc atcagtcgca caaccgacgg cgacccggcg
    29641 taccgctttc ctccgacgat ctcatcgctg ccgctgtcat agacgaagcc ttccatgttg
    29701 acgtcgtctt ctttctcgcc tttcttgaga cctgctttgc cccatacgag aacggacacg
    29761 ccgttttcag ccgcctgctt gaagacggct tttgacccat ttccatgccc atttccgaca
    29821 tcccgcacct tgatacccaa gctgggaagg tccatcagct cgaataccag cgagcgcttg
    29881 acgtcctttt tcagcacctc ctcgaggcgt ccgccgagcc agtcaggccc gccgttgttt
    29941 tcgatgctga ccacgccgag cggaatcttc tgaaagtccg gcctcgtcgc ctcgagaaag
    30001 acatcagccg catcggactc cagaatgccg gcagctgcag ccaacagaca aagcccggac
    30061 aagacaaggc tcaacacgat tcgtcgattc atcccgcgat ctccccgact cgaaacacca
    30121 tttcaacgtc ttgataggcg tcgctcatat ccgcgggaaa cgcaggaaat tgacgcggct
    30181 tcaagatggc tcgctgaccc gacatgtcga aatagctgtt cccagacgtc tgttgaatta
    30241 ccacatcctt gacggtcccg ttccgataga accggaatcg gaccactgca ctgtacgttt
    30301 gtccggtcac atcgatggga ggcggctccc attgactcct gatgatcgct tccacacgcc
    30361 cccagaacgg attcgtgccg gatgatcccg acgttttcaa ttgcgtctgg ggggccgtta
    30421 cgtgtggcag ggcggcttcc tggtgaggaa cggctttgac gggtgcttct ttcgggatct
    30481 ccagtttagc agccggtttg aattccttga ccttcttgag ctcctcttcg agttcgcggt
    30541 cgagctcttc gctgatcgac gttcgctgcg gagtcttcgt caccggatcc ggcatgatat
    30601 ccggaatgcg agggaggtcg ggaacatgga ctttcgcttg cacctgtgga cgaggttgtg
    30661 ccaccgattt gaccggcgtc aattctccaa atttcggcgc atccgggggc aactcaagat
    30721 ccttgagaat atctttggcc acactggtac gcgccggcgt cgaaacgggg ggagccgcgg
    30781 gggcaggcat tctgacgggg ggagcggaaa ccggaaccgg cttgggttcc agtcgtttga
    30841 cgggttcgac cggcctactc tgctccacgg gtttcatcgg ggtgggtaaa ctgaccaggt
    30901 tcacttggac tgaggccaaa ggccgttcac cgtgaggagc cagccgaatt cccattgcga
    30961 tcagcagagc gaccaggtgc agaacaaggg agatggtaag cgttcgaccc aggcgagcaa
    31021 gaaaccggtc ctgctcgtca tgatcgaacc agaaagccga ctgcacctgt gcgcgagcga
    31081 ccatcccaac atccctctca gcagcgacgt gtctagctct tacgagaggg tccgcgcgga
    31141 atcgatgccc ccccgatgtg ctccggtccc accggttcgg tcaccattcc gatcttctcg
    31201 attccggctt tcttcacccc atccatgacc tggacgacga tgccgtaggg cacctcacga
    31261 tcggcccgga ggtagagcga cacgtccgca tgctcgtgtt tcaagacttt tagtttttgc
    31321 tcaagctggg cgacgccgac gggatcctta tccaagtata gccgttgatc cttttcgatg
    31381 gtcagcaccg ttcgcatttc cggcttgatg gtgttgctcg cagatttggg gagattgatg
    31441 tccataccgc ggtacaacat cggcgcggtg accataaaaa taacgagcag cacgagcacg
    31501 acgtcaacca gggggatcac gttaatctct gccatgaagc ggcgctggcg cgactcgaac
    31561 atcatcgttg cgctccgacg ggaatcggct tggccttgat ggttggaatc gagagtgacg
    31621 tcatcagctc gatggtcacg gtgtccaatc gaaacgcggt ccggcgaatt ctagacagga
    31681 aataattata ggcgatgacc gccggaatcg ccgcaaacaa gccggcagcg gtggcaatca
    31741 aggcttcagc gacgccggga gccacagccg caatgctggc cgtgccttga gcaccgattt
    31801 cacggaatgc atcgatgatc cccatcaccg ttcccaacag gccgataaaa ggcgtgatat
    31861 tgcccgtcgt cgccagaaat gggagatagg attccagtct cgccagctgg ctgtgggaca
    31921 aatgggcggc ggttcgttcc acgagctgac gatcggccac acccgccccg tcctgtccac
    31981 gcaaccccgc aacgcgatcc atcacgcctt caaacaagat ggcagcgggg cttgtctcca
    32041 tgcgtcgcac ctgccggcac agatcgtcca aatccttcgc cttcgccagc agagccatga
    32101 actgatgatc catgcggtca gctgccgaaa acgcacgcca cttgaggaag ataatcgccc
    32161 agcacacgac ggagaacaca aagagcagca gcagcaccac tttcgaaact gcgcccagtg
    32221 agcccagaag gccggtcaag ccgatttgaa acatggatcg ctacctttcg tggtctcgcc
    32281 gaagagtcca cctacccttg ggagtgtaac aaatgcgtga ggaagtgcgg cgctggcccc
    32341 cgtgaccact ggcctaacag gggcatcgct caagacggcc tgactccatt gaaaccgctg
    32401 aaaccgcctg cggaggtgta tcccaatggc catatatgcg ggacgaaaaa gatggcgggg
    32461 ccgacgggat ttgaacccgc gacctccaga ttgacaatct ggcgtcctaa accaggctga
    32521 acgacggccc ctcgtgactc tacctattgc ggatgatttg attgatcaga cctatatata
    32581 gcggatggtc cagtctgtct agaatatttg ttcctactgc atgaacgaat ccgtcccgcc
    32641 gtgggtggta ggcggaacag ggatcgaacc tgtgacctct gccttgtaag ggcagcgctc
    32701 ttccaactga gctatccgcc cgatttcttt aataagctgg cggatgctat caagtccgtt
    32761 cattcttgtc aaccgaatct cgcactgttc ttgcacattc ttcagctact tccgggcttc
    32821 tgtgacggct cgaccatcct tcccgcgtct ctgccgcggg aagaaatttg catggacttc
    32881 tttgagccgc tcgcgttcga cgtgcgtata gatctgcgtt gtcgcaatgt cggcatgtcc
    32941 gagcatcgtt tgcaccgatc gaagatcagc acctcccccg agcaaatgcg tagcgaacga
    33001 gtgccgcaac atgtgagggg acacggcttt cgtcaacccg gcccgttgag ctcgtgcgcg
    33061 cagcgatttc caaaatgcct ggcgcgagag aggcgtcccg cgacgcgaga caaacaaaaa
    33121 tcgcgacgcc ctctgtctca agaatcgcgg cctgatcgcg tccagatact ccagcagtac
    33181 ccgccgggcc ggctcgccca tcggcacgat ccgttgtttc gaaccctttc ccgtcacacg
    33241 cagatagcca acctcaaggt tcacgtgcaa cgtctcgaca gaaatcaact cggagacacg
    33301 aagtcccgtc gcgtaaagta attccagcat cacacggtcg cgttgatctt ccagcgtcga
    33361 ttgagcggga ctctcaagca aggccgtgac ctcgcgcgcg gacagcgtct tgggcagacg
    33421 gattccgcgc cggaccggag ccgcatcgag cgccgtatag tcatcgataa tctgctcttg
    33481 acggaggaag cgaagccatc cgcgaacggc ggataaacaa cgggccgacg agacggggga
    33541 caaccgcaca gcatgcagcg aacccataaa gctccgcacg gtgtcaggcg ggagccgctt
    33601 tcgcaagccg atcttccgct cgctgagata gcgctgaaat ttcgcgagat cgcgccggta
    33661 ggcttcgatg gtgttacgag cgaggccgcc ttcgacacga agatgattga gataccgctc
    33721 gaccagcgcg tcggtttcca tttcggcgat gatagccaat cgttctcatg aacacaacgc
    33781 gacggcgggt gcgtgaaacc ttgacaccct ccggggattc cgatactagt cactcttgac
    33841 gatcttcatg cgtaccagcc acgatcgcca ctttatccgt cgcgccctac ggctgcccga
    33901 tctccatcgc atacagcttc caatcgccgc ttgtgtcgcg ctcaccctgg cgcttgtcac
    33961 gtttgccgta ccgggacgcc atcccgtcgc tgccgcggag acggccaagc gtgcgcccgc
    34021 cagccatccc tccctgaatc aggccaagcg gttgatcgat gccggacagg ctgaagaggc
    34081 catcacgatt cttcggcgat ttctcgggac caacccgaag ccggagttcc ttgacgacac
    34141 atacctactg cttggggcag ccttataccg aggacagcaa tatccggaag ccctcaaata
    34201 tctgcagcaa cttcaaacag aattcccgtc atcggaagtg atcgaccgtg gcaagttgat
    34261 gctggcacgc acccatgccg cgatggggaa ccccgatctg gcgttgccga ttctcacggg
    34321 cctccgcgcc ctctcacagg acgatgtgac gaagcgcgaa gcggtgcatc tcagtggaga
    34381 gctgtacttt caaaaaaagg agccggctcg ggccattcag gcctggctag acgagatcgc
    34441 gttgagctcg gacgagcaag gcgaagaaat ccggaacaat attaccgagg tgatcaccga
    34501 atcctttgac aaaaaagcgc tcgagcgaat ccgtgaagcg tatccgaaac agtttcccgg
    34561 ggatctggcc gccctacggc tgatcgacct ctacatggga cgtggcgaag atcaccaagc
    34621 gtctcggcaa atccagcaat ttctcgccca tttccccact catccccaga gaggccggct
    34681 ctcggaaatg ctggaagctc tgcaaaccaa actgaagtcc agccagtacg tgatggcggc
    34741 cgtcctgccg ctgtcaggaa agctctcgtc attcgcgaat gatgtgttga gcggcgtcca
    34801 acttgccgtg gaagcaggca gcgagcgtgg aggggggccc tccatcggct tgctcgtcaa
    34861 agatctcgat gcttcccacg cgtcctttct cgacgatttt tctcatctgc tgtttaatga
    34921 ccgtccgatc gcggtcattg gtccactctt gtccaagaac cttcccgtga tggcggagct
    34981 ggccgaacgc tcgcacacgc cgctcctgac gccgaccgct atgctgccga acgtccgacg
    35041 gctgggcagc tttacgttca gcacggcggt gacgtaccaa ctgcaggcac ggaaaattgc
    35101 cgcgtatgcc actgtcgacc tcggcttccg ccgattttgc attcttcacc ccgacacggc
    35161 gtacggacgc gaaatggccc gcttgtttgc ccaggaagcg cggcagcgcg acggagagat
    35221 catcgcgatc gagtcctata aagaaggcga ctcggatgtg ggcgcacagt tgaaacggat
    35281 gaaagcggag gacctcaaga aatacgggct cgcggtccag gtagatccgc tgaaagtcgg
    35341 cgggaaactc acgaagctcg acaagaaagt gctgtacact cccggcttcg atgcggtgtt
    35401 tatccctggt cgagccgccg acgtcggctt gatagccgca caactaaact ttcacgacat
    35461 gaaggtcccg tttcttggga gcaacggttg gaacgccccg gattttgcca gaacagcgga
    35521 tcaatccatt gacggtgccg tcttcgtcga cgggtttttc gccgaaagcc cgaaccccag
    35581 cattcaagat tttgtcgacc gctataagaa gcgcttccaa agcgcgccaa cactcttcgc
    35641 catgcaggga tatgatgcgg cgaaattcgt ggtcgacgcg atcaagaaag gcgcgacgtc
    35701 cggggacgca ctacgcgatt atttgacgag ccagcaagac ctgcccgcgc tggccggacc
    35761 ggccgccttc gctcaagacg gtacactcaa ccggcccctc ttcttgatac aagtgaaacg
    35821 tggacgtttc actcaagtgg actaagcatg gtgaacggtg ggatggccac atcggtcgcc
    35881 tcgaaagggc gcctccggaa cgagttcctt cttcatgctc agccctgaag tcacggaatc
    35941 gtaggcgatc tacttccatg aacgacgtca cgcagatcct ccatgccgtc tcgtataaag
    36001 cacttccctt gctctttgcg atcgtccttc atgaatacgc ccatggatgg gtcgccaata
    36061 gatgcggcga ttcaacagcc aaacttcagg ggcgtctcac gatgaatcca ttggcgcata
    36121 ttgatccgtt cggcaccatc atcgttccgt tgatttgcct gatgctgccg ggcagctttc
    36181 ttcttgggtg ggccaagccc gttccgatcg accccagaaa catgagacgg ccgcgacgcg
    36241 acatggccat tgtcgccgct gcgggacctg gcatgaatct cacgctagcg gtagccagtg
    36301 cgggactgct ggcgctgctc ttgtcggtcg atccgtcgct gatgacgagc gattcgagga
    36361 ccgacacgct ggaaaccgcc ccctcgtccg cgtttcgatt cttgctgccg gtcgccgtca
    36421 tggcattgta ttcggtcttg gtgaatgtgt tcctcgccat tttcaatctc atgcccattc
    36481 caccactcga tggcggacga atcttattga gtctgctgcc tccgcgcccc gctctggccc
    36541 tcgcacgatt ggagccttat gggatgatga tcctggtcgg tctcctcgtc tttgaccggg
    36601 aactccgcgt catccacacc tttaccagta catttgcgtc ggggctttca ggtacacttc
    36661 tgtcgacggc gatcgggttc agtgcaggag cctccccatg acaagccgac cgaaacgagt
    36721 gctgagcgga atgcagccga gcgggctcat gcatttgggg aattacctcg gcgcacttca
    36781 gaactggaag caccttcaaa ccgaatatga gtgttatttc ttcgtcgcag attggcatgc
    36841 actctcgacg aattacgccg acacctcccg aatcagagaa ttcgtgcggg agctcctgat
    36901 cgactggctg gcagccggaa tcgatcccaa ccggtcaatc gtcttcattc agtcgcgtgt
    36961 gccggaacac gccattctgc acctcttgtt ttcgatgatc attccgatgt catggcttga
    37021 gcgcaatccg acgtacaagg agaagcagga agagatcaag gaaaaggatc tgacgaccta
    37081 cggctttctc ggctatcccg tgctgcaagc agccgatatt ctcctgtaca aacccgattt
    37141 cgtcccggtg gggaaagacc agctccctca cttggagctg acgcgtgaag tggcgagacg
    37201 attcaacgat ctctacaaac attcggtctt tcccgagccg aaagaacacc tgaccaagtt
    37261 cccgaaagtg ccgggcaccg acggcaggaa aatgagcaaa agctacggca acaccatcaa
    37321 cctgtccgac actgaaccgg tcgttcggca gaaattgaag acgatggtga ctgatcccgc
    37381 tcgtgtcagg cggatggacc ctggaaatcc ggagctctgc ccggttttcg attttcacaa
    37441 aatgttctcc tcgctgccga tcatcgagca gatcaatcgg gaatgccgta cggcggaaat
    37501 cggctgcatt gattgcaaga aactggttgc ggatcgagtc gtggaaacct tggcgcctat
    37561 ctgggacgct cgagcggctc tcctccagaa tccgtccggc atcgaagacc tcgtgcatgc
    37621 aggcagccaa agggccgcca ccatcgccaa ggccacgttg caggaggtga ccgaggcgat
    37681 gaagatttga gagatgaccg gccagtgagt cgcccgtgtt cgcgcaacgc gcggtcactg
    37741 ggaagaggcg gcgtcttgcc gccgcgacgg cgggtgggtg agaaaacgac cgtcgttgga
    37801 cgcgcgcagt tggagagccc ttcgggccgg tcatggagag ctgaggatgg aatcacatgg
    37861 agggaagaag ctcctcattg cagttgtcct ttggcagctc agcggagacc atgcctgggc
    37921 agtggatcct ccctgcgacg cctttccgcc tgcaaaacaa ctccgctgcg tggaagtctg
    37981 gaaggacctg tacaaagagg acgggccggc gatcgcacaa ttcgggcttg accagctaaa
    38041 gcgccgggaa gaaggcaaga ttaccgccca gcagcacctc gcccaaaaca tggacttcat
    38101 caaacaggcg acggaaagac gtctcgcgcg attaaacgag cgcatggcga gggagtgagc
    38161 gcagccgggt gactcctcac gtcctgaggc gacgaagctg tcggcgacgg agcctccctc
    38221 cgagtgtcag aggtcctggg gcttcggttc ctcaggaacc acgcccgccg agttcaagag
    38281 atcccgcccc acaataatcg gcgccttgaa gtgaatagcc agcgcgatcg aatccgaaga
    38341 gcggctgtcg aagactttga cgtcgccacg gaaggaaacg ctcagcgctc cataataggt
    38401 gccgcctttt aaggtaatga ccgattgcgt caccttcgca ccgaacgtgt caagaatggt
    38461 atgcatgaga tcgtgagaca agggccggtg caactgctcg ccatttaacg cgccctggat
    38521 agagaccgcg acggtgagat cgacaaagat gggaatagtt ttgccttccg cctgcagaag
    38581 taccaccggc ccatggtcgg agatgcgcac cttcacgtcg gcgatggtga cttgatccga
    38641 ttgtggaccc gaggccgcct ctgcgtgcac gatgggtggc ccccatgcaa aggacaacac
    38701 gaacaacgcc agccatcgag cacttgttct catagccacc tcctcatccg ttcaggcgcg
    38761 aacgaattgg ttcctatctt tctcccatcc gatctccgtt tcaggcccat ggcccgtcac
    38821 gacggccgtc gcgtcctcga gcgtgtacaa ccgttcaaga atcgagcgct cgatcgcgtc
    38881 aaaatcgccg ccccacagat cggtccgtcc gatgctgccg cgaaagaggg tatctcccgt
    38941 cagcacgata cgcgccgccg gaagatgaaa gctcatggat cctggcgtat gccccggcgt
    39001 gtgaagggca ataaggcgaa cctgcccgat gtggactgtt tcctcgtctg tcagccaata
    39061 gtccggcggt ggaaccggca tatacggaac tccgaacgcc cggcattgaa gttcgagcat
    39121 gtcccacaac gcgtgatcgt ctttgtgcaa gcacaaggtc gcggcggtcg ccgccttcat
    39181 ttcgccggac gccaaaaagt ggtcaaaatg cgcatgggtg tgcaggatct tcgtcaccgt
    39241 cagtccgagg tcttgcacct cacgaagaat ccgttcggca gcgccacccg gatccacgac
    39301 aacggccagt ttggtcacgg ggtcgccaag gatcgagcaa ttgcagccga gcggcggtac
    39361 ggcaaacgtc ttccgaatga gttctgtcat cgcaaaaggc tctttcagta tgctaacgcg
    39421 caggccgttc ggctgcaact gaggagaagg aggcccgtga cgaacgatga gccaggcggc
    39481 gaggcaagac cgtccgctac tgaggccgac ctgcccagac taattcgctg tcgaagatgc
    39541 gccagtgggc tccggaccga tggagccata ccgcaaaacc ggctccagct ccagtcggtc
    39601 ggttgtattc gacatagatc agggcctgct cgcggccgag catatatgcg acgcgtgaga
    39661 aggccagtcg gtcgaggata gccggcggtt cgtcaagcgg ctcttcttgc atgaagcgag
    39721 gactctccga cacgaggatg gacggcatag acgcttcctg ttgatcgacc ccatccccag
    39781 aaacgaaccg atatcgcacc ccaaacccga aacgcccatc gagcttcgaa ggcgattggt
    39841 ttttaacgag gaagtcacgg atgagctcgc ccggtagacg accgtcgaaa atctcatact
    39901 gatggaacgt ttccggcctc agagggccct gttgctccgg atgtaatcgt gtcagggtga
    39961 accgttcgag gacgataagt tttgtttccg gcgtaagaaa tttctcgtcg acaacgagat
    40021 cgtacaaagg ataatcctcg gcaggtacgg cggcagacgt gtcaggtcct gtctccgccg
    40081 gtgcgagaga tgcagagccc atggacagga cacaggtgag cgccaggagg ctgaaaccgg
    40141 tcatgcgaga gcgcatggac gagaatagcc tggcatcata taggggtcaa tcaaccagaa
    40201 tgtgaattga ccatggacaa gagaccccga agcatggata atccgtccac tcatgtggtg
    40261 ttgtcgacat cgctcgattc tcctccccct tgtcctgacg actttcttgc ctttgtccgg
    40321 atgtaaaccg gatgaatctc ccttcagggc cgagaacgac ctactcaaga aacaactcgc
    40381 ccggcaggag tccctggtta cctcgttgca agacgggaac aaagtcatgc agcaacagat
    40441 cgaccttctg aatcaggaga tccgtgacgc caagaaggca gcagaaagcg cgagagctga
    40501 agccaaagac gcagccgaac agctcgacaa acagctgatc caggcccgca aactgacggc
    40561 agacatcaag aagacggccg tcgagcaggc cgcccagagt attcagctgg aacaaaaggg
    40621 cgcgcaattt gaagatctcc cgcggccgtt gaacgccgtc accaagacgg tggaggaagc
    40681 gctggcgcgc aacggctatc aggtgaaagt cagtatcaaa gccgatcaga aggccgtaat
    40741 cgttacagac cgcaaggtct ccaatccggc ttcactcgaa gtggccggtt ttcgaaatca
    40801 gtatgtgatc gccgtccaag cgcagccgtt aaacgtcacc cgtctgagcg tcaaggccga
    40861 attcgaaaag ctggcgcagg gcgggcggat cttgagcgtc agtgcggagg aaacggcaga
    40921 aatcgaacga cgacttatcg gcgaagtcag caaagctctt tccgctccca gcaagacctg
    40981 atgtgcagtg ttttcgtgtg gcttgctgga tctgtcctgt cgctcatttc cattaacgca
    41041 aaccaagcgg caagcgcgca caagccttct ccgcaactgg ccggtcatct cgcctcgatt
    41101 gcggggctgg cgcagtcggc gccgacggtc cagatcccgg ccggtgagtt cacggttggc
    41161 agcaagcgaa tcgacgacga cccctatggt ctatggacgc agttcgacga taccgaactt
    41221 ccacaacagc gtgtctggtt ggacgcctat gcgattgacc gtcatgaagt cagccttgcc
    41281 gaatatctct cgcatctccg acagaccaac cagcatcccg ccgacgagtt acaaaaactc
    41341 atttggcatg tcatcaccgt ccattcggtc tcggatcaca cgctggcgcg gtggccggct
    41401 ctctacatca cctggcacga ggcggcgaga ttttgcgcat caaagggaaa gcggttgccg
    41461 accgaagcgg aatgggagaa agccgcccgc ggtccagacg gccaactttt cccctggggt
    41521 gatgccgcgc caagctctga acgggcgatg tttggccagc atcacgtgca tgaaattccc
    41581 attttggctg ctgtggactc gcaccccgag ggacgaagtc cctacggcct gcatcacatg
    41641 gcaggcaatg tggcggagtg ggtgcaggat tggtttggct tggactacta cgcctatatg
    41701 ccgggaaaga atccaagcgg tccgtcaagc ggccgctata agggcgtgcg cggcggctcg
    41761 tggaagagca agattattat gcttcgcacc gccactcgaa gcggctttag tcccgaccag
    41821 cggtcggcca ccattggttt tcgctgtgca caaccttctc cgaaccctga tccctaacga
    41881 caacgacccc cctcgtgctg ccggccagta agatagaatt ctggccgatc gcttggccga
    41941 ttgtggctct catgctaggt tggcaagaat gaacgcggcc tcgatctctt tgacccgaat
    42001 ggtcttgatt ggatttctcg tcgcaatcgt gacgctcgtc ggctgcgcca cgtcgaccgg
    42061 tgaccgctcc tctcgaaccc tcaccggtca gccctgctgt aagtccaccg aggctacgtc
    42121 ccggcgcgct gccatcgtcc aaacggcggt caggcttgtc ggggcgagca cgattcacat
    42181 taagggccga cgcattgcct acgactgcgc cggcgtgacc cgagcaattt ttcttgagca
    42241 cggcatcgac ctatacgaca gcgccgtgcc tgatccaaaa gccaacggcg tgaagctgat
    42301 ccatcgtcat gtcaaccggc acggcaaact tcatcagggt ccggccgtcc ggcccggtga
    42361 cctcgtcttt tttgacaaca cctgggatta caatggggac ggcatggcca acgacccttt
    42421 gactcatgtc ggtattgtgg agcgccagga agacgacggc acaatagtgt ttatcagtcg
    42481 ggttgccggc gccatcgagc gctaccggat gaatttggcc ctcccccacg tccatcgtac
    42541 gacggaagga cgcctcctta acgactatct ccgtcgaaaa gacttcgacg atcctgccag
    42601 ggccgcctat ctgaccgggg agttgtttgc cggcttcggc agtccgaccg ggctgtaacc
    42661 tcccaggcgg cagggcggac caagcacttt tgcgtaattg cgctatactt gctcctatcg
    42721 ggcaccgtgc agttttattc ttgaggagga gctatggcga agacagcgac ctattgcagc
    42781 gagtgttaca acaaggtcgg ccgggtcgaa gacgcagtgg tcaaagcggc agaagcctcc
    42841 gggaaaacgc ccatgaccgg gcaaggcacc tgctgcaagt gcaacaaggc tacgatcgtg
    42901 gtctactacg agggctaaac tttaagcccc tcgatggcct catggcgcgg aaatccgtgc
    42961 taggcatggc cactattttc cgtctcatcg aattcgttga tgaggtcttc taggtcatcg
    43021 agttcatcgg gcgggatttc ttgaaattcg acgccaaacg tctgctcgtc gacccattgc
    43081 accgtcgcct ccggaatcga caacggttcc tccaaacccg gaatcacgag acggaccacg
    43141 atcgtcttcc ctgggaagac ctcgacatca ctcgcaaatt tcgctccgcc gagcgagagg
    43201 tcgagcgtgt gtccttcgac ttcatgagaa ttccccaaca acaatccctg aatctgaatc
    43261 ggaacacgcc ggtgttttcg tcgttccacc ttaggcacct ctcgaagacg gtaggaagaa
    43321 gaatacctcg ctcttggacc gaaatgcatc cgtctttatg tctgcatcaa ggccgctggc
    43381 cgtttcgtac aggcctgcta cacttgacct atggaagcgt catctcacac gacggcgctg
    43441 gttcccgtgg atccacgtcg caggactatc ctgtcgatgc gggagagcct catcgggctt
    43501 cacggtgccc tcattcttgc ggagcaacaa acctatgaac gcgtgagtgg acccgtgcaa
    43561 tccgtggaag tgttgatcga gctgctccaa aaggaccctt ggttcacctg gctgcacccg
    43621 atcgccgatc tgcttgtccg catggatcac ctgctgcaag acgatacgtg tgacatcacc
    43681 ggcgacaatg tcgcgcatct cctgggagaa attgacagct tgcttcatcc atccgtcgaa
    43741 ggagaaggat tcgaacgagc ctactatgaa gcgctcgagc gggctcccga tgtcgtgctc
    43801 gctcactttc gtataagaaa gttgttgacc aaggctgcat agctccggtg gctttgttat
    43861 gatgcgagtg aggagcagcc atggggtgga tggaacaggt ccggagcagc ctgatcgccg
    43921 gcgcgctgat ggcagtctgg gtcacacagc ctcctttggt actcggcgaa gtaccgagcg
    43981 acaatcggat caacgccgga cgtgacggga cacgcggcgg cacggactcc ctttctgccc
    44041 ggcaatctgg cgctgcgccc atcctgcatc ccgagcggaa tgcccatcaa cccacaatga
    44101 cgccgctcgg agtgccgtca ccgcctcacc agctcacccc ggctccggtg cttccctttc
    44161 accctaacgg ccacctcatg ccttccccgg cagtgccccc gaatgcgccg ttgggtgatc
    44221 cataccatag ccgctgacgg cggacgctcg tcgttcactg taatggtgac ggacaaacac
    44281 atttttccta cgacctgata caatggacgt atggcacgaa cactcgaatc ctcaacggaa
    44341 gccggcgcgg gtctgcgaca cttgctgatg gaggttcgcc gcggactgct aggcctgcat
    44401 aaagcgctga tcgtggccga gcagttgacc tatgagcgca tcaacggccg cgtagggtca
    44461 acgggacaac ttttgcagct ggtgttgaac gacccatggt ttacatggct ccatcctctc
    44521 tcgcagctgg tcattcgcgt ggatgaatta ttggaggaag gagaggaact cacgaccgtt
    44581 gaagtggaac attttcttgc cgacgtccgc gcgttaatcc gtccctcaga agagggcgac
    44641 ggcttcgagc gcagctacta tgaggcgctg caacgggaac cagacgtcat ttttgcgcac
    44701 gtggaagtga agaaactcgt caccaaagcg gccgcttaat cagctctacg cgacaggcgg
    44761 cttccagccg cctgtcgccg ctctccccaa actctcccga ctattgattc gctcgagcct
    44821 tctcgcggcc ttgctttcca tggtgaccgg ccgcttcatc cgcgcgatcg agtccatgtt
    44881 tcttgttcgc ctttttcgca tcgcctttgg tctttccttt cgccttcccg cccttcaccg
    44941 gcttgtcggg cttcttctcg tcttcgccca cgctcggggg cgtggtc
//
LOCUS       DASSCQ010000009         6082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1089075, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000009 DASSCQ010000000
VERSION     DASSCQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089075"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFH05_00730"
     CDS             <1..709
                     /locus_tag="VFH05_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET6676769.1"
                     /translation="DERGWAFRNGPGHSQDPINGFRYLSEAYKATDPTYLGRVTVPAL
                     WDTVTKRIVSNSDDDLMRMFNHEFTAFTPSRLDLYPADLRNEIEEWNAFLYANVNDGV
                     YRAGFATTQAAYEKAVRRLFEALDRVEQRLADRRYLFGSQYVETDWRLFVTLIRFDAV
                     YHGHFKCNIRRIADYPNLFGYLKDLYQTEGIADTVNFDHIKRHYYVTHDDINPTRIVP
                     LGPQQDLSAPHGRGNFD"
     gene            837..1670
                     /locus_tag="VFH05_00735"
     CDS             837..1670
                     /locus_tag="VFH05_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676770.1"
                     /translation="MRNAFRFGECCVCILIAAATLSSQSASAASPKEATFYAREHRFD
                     GPETIPAGQTTIRLRNRGKEPHQLQLLKLEAGKTPAELGVALQSGSQSIPPWAKQMGG
                     PNAVGAGQAAEATLYLEPGSYVIICGIPGKNHQTHATLGMQKTLRVIETKSPPPEFRG
                     NFHMAMFEYEFVVVQPLRKGRHTFYVVNRGNQTHQASFIRLDPGVSTEDVLTAIHQDS
                     PTRLPGTLIGGMSGLEPGRDGTFTANLTPGRYAIMCLFSNTAMSESHAAQGMVLNFTI
                     E"
     gene            complement(1758..2492)
                     /locus_tag="VFH05_00740"
     CDS             complement(1758..2492)
                     /locus_tag="VFH05_00740"
                     /EC_number="1.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004128 - cytochrome-b5 reductase
                     activity, acting on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-cytochrome b5 reductase"
                     /protein_id="HET6676771.1"
                     /translation="MDSHIITRIKTKLPSPYQLTATNQDTHDTKTFCFALPEDATLDM
                     LPGDHLYVHATINGKAVKRPYTPSSVPGTTGYFDLTVKRYENGTVSRYLHDREIGDSV
                     LMSGPNSGGHWVDGMAKQVGCVAGGTGITPIISIIRWILAQGLPVELSLVFANKSEAD
                     IIFRDEWEADEREHANFHVFHVLEQPPAGWTQGRGRITEETLRTHLPQPGPQTVVFLC
                     GPPMMVDALEGTLKGMGYPEQSIILP"
     gene            complement(2550..3182)
                     /locus_tag="VFH05_00745"
     CDS             complement(2550..3182)
                     /locus_tag="VFH05_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6676772.1"
                     /translation="MRITASLQSRIGLFLFSCLWLSGCAHLAYQHMNDPSRDAWQKPK
                     DVVERLGITPGSRIADVGAGGGYFTWHLASAAGPQGRVYAVDIDETGLKIITKEMQMR
                     HITNVVPVHAEPTDARLPEPVDLVFSCDTYHHMDDRVAYFKSLTRYLKPDGRVAILDF
                     HPHGFFSGLLGHGSAAEEVRREMESAGYRRTADFDLIDRQHFQMFMRSGF"
     gene            3511..4011
                     /locus_tag="VFH05_00750"
     CDS             3511..4011
                     /locus_tag="VFH05_00750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6676773.1"
                     /translation="MAKLSRYRVAMVALSALIASLGLFALSGHAADSRAISDGSKVTL
                     LYQITVPGDERFEVRDLSQFVQGQHQMLPAVEQAVTGMHRGEKTEVKLSEDQAFGAYD
                     SSKKTMVPKKELPAGAKTGDIIEDRKTGKQATITQMSDTDAVMDYNHPLAGKPLLVSL
                     TILEVE"
     gene            4163..5308
                     /locus_tag="VFH05_00755"
     CDS             4163..5308
                     /locus_tag="VFH05_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET6676774.1"
                     /translation="MSLYSLIVVFSLLTSLLTSAAQSSAGNQIYRYKDDSGTVTFTTE
                     FQSIPEKYRREAVPLATNPPPPPERRHEPSVRIVTSSGEFRMGNYDTRTDATRMAIEA
                     AKRDALEQVATYLESVTEVKDLDVTRDEIRTYTAGVVTVLNQQTSTRLEKGVAIIHVD
                     LTVQADQQEVIQAITALRENESAKVQLASLRTETEQLRLQLDAANQALATALTAEQVQ
                     ALTLERQQLLNQIQADALVARALTDFAYLTPIKVRQVNDLLLQARQLHPANQHLQAVE
                     QALATKTGPSALPLVVPSPGNAPSASPDVVPAQRSPLVAPLIESPLATPLPPSPLATP
                     LPPSPLAAPLPPSPLAAPLPPSPLAKPLGPSPLATPLAPSPLAAPLR"
     gene            complement(5673..>6082)
                     /locus_tag="VFH05_00760"
     CDS             complement(5673..>6082)
                     /locus_tag="VFH05_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676775.1"
                     /translation="LNVGQSSRSLIDYFVDTNALDAMDAMLLESRRSLLAGMGIPIAR
                     QDQEMRGRLPVNDPRQCGCEVRHIALRQLMYAGEESDGLAERVTQIKTKFWIAEEEQN
                     DLFTAAKLLMKMVDDKKLLAEESLRTSCAVERK"
BASE COUNT         1331 a         1843 c         1635 g         1273 t
ORIGIN      
        1 agacgaacgt ggctgggcct ttcgcaatgg accgggtcat tcccaggatc ccatcaatgg
       61 attccgttac ctcagtgagg cctataaggc gactgatcct acctacctcg gacgtgtgac
      121 ggtgccggcg ctctgggata ccgtaacgaa gcgcatcgtc tcgaattccg acgacgatct
      181 catgcggatg ttcaatcacg agttcactgc cttcaccccc agccgtctcg atctgtatcc
      241 ggcagacctg cgcaatgaga tcgaggaatg gaacgcattc ctctacgcga atgtgaacga
      301 cggcgtctac cgtgccggct tcgcgacaac acaggctgcc tatgaaaagg cggtgcggcg
      361 gctctttgag gcgctcgatc gagtcgagca gcggctcgcg gatcgccggt atttgttcgg
      421 ctcgcaatac gtcgaaacgg attggcgcct cttcgtcacg ctcatccggt ttgacgccgt
      481 ctatcatggc cacttcaagt gcaacatccg ccgcattgcc gactacccga atctcttcgg
      541 ctatctcaaa gacctctatc aaactgaagg gattgccgac acggtcaatt tcgatcatat
      601 caagcgtcac tactacgtca cccacgacga catcaatccc acccgcattg tccctctcgg
      661 cccccagcag gacttgtcgg cccctcatgg acggggaaac ttcgactaga ggagcccacc
      721 gatccgaatg gctctattgc taaatcttgt aggtaaagtt attactattg tcgccggtcc
      781 gttggtctag cccgtcgctg aggccgcgag gaacatgtcg ttctcaggga ggaaccatgc
      841 ggaatgcttt ccggttcggc gagtgttgtg tctgcatact gatcgccgct gccacgctct
      901 cttcccaatc agccagcgcc gcgtcaccca aggaggcgac cttttacgcc cgggagcatc
      961 gttttgacgg ccctgagacg attccagccg gccaaacgac catccgcctg cgcaaccgcg
     1021 gcaaggaacc gcatcaactg caattactga agttggaagc cggcaagaca ccggcggagt
     1081 tgggtgtcgc cctccaatca ggctcgcaaa gcatcccgcc gtgggccaaa cagatgggag
     1141 gcccgaatgc ggtgggagca ggccaggcgg ctgaagctac attgtatttg gagccaggct
     1201 cctatgtgat catctgcggc attccaggga aaaaccacca gacgcacgcc acgcttggta
     1261 tgcagaagac attgcgggtg atcgagacga aatcgccgcc acccgaattc cgtggcaact
     1321 tccatatggc gatgtttgaa tacgaatttg tagtcgtcca accgctcaga aagggtcggc
     1381 ataccttcta cgtggtcaat cggggaaacc aaacccatca agccagtttc attcgtcttg
     1441 atccgggcgt gtcaaccgaa gatgtgctga ctgctatcca tcaggacagc ccgacccgtt
     1501 taccgggcac actcatcggt ggaatgagcg gactcgaacc gggacgagac gggaccttta
     1561 ccgcaaacct gaccccaggc cgctacgcca tcatgtgcct attttccaac accgctatga
     1621 gcgaatcgca tgccgctcaa ggcatggtcc tgaacttcac catcgaataa ggcggagccc
     1681 ttcagcggat gcccgatgtg tctgtagtga agcggacgcc gagtaccgct gcgtcaagcg
     1741 tgggcgcaga tgaatcgcta gggaaggatg atggactgct ccggataacc catgcctttg
     1801 agtgtccctt ccagcgcatc gaccatcatc ggtggcccgc agagaaagac cacggtctgg
     1861 ggaccaggtt gaggaaggtg cgtgcgcaag gtctcttccg tgattcgtcc tcggccctgt
     1921 gtccagccgg caggcggttg ctccaagacg tgaaagacgt ggaagtttgc atgctcacgc
     1981 tcgtcggctt cccattcgtc acgaaaaata atgtctgctt cgctcttatt cgcaaaaacg
     2041 agagacagtt ccaccggaag gccttgcgcg agaatccagc gaataatgga aatgatcggt
     2101 gtgatcccgg tgccccccgc cacacatccc acctgcttgg ccatgccgtc cacccaatga
     2161 ccgccgctgt tcggcccgct catgagcacg ctgtcgccga tctcccgatc atgcaggtac
     2221 cgtgaaaccg ttccattctc gtaccgtttg actgtgagat cgaaatagcc ggtggtgccg
     2281 gggacagagg acggtgtgta aggccgcttg accgctttcc cattgatggt cgcatgcacg
     2341 tagagatgat cgccgggcag catgtcgagc gtcgcgtctt caggcagcgc gaaacaaaag
     2401 gttttcgtgt cgtgcgtgtc ctgattggtc gccgtcaact gatagggcga gggcaatttg
     2461 gttttgatgc gtgtgatgat gtgcgaatcc atacaggata ttttacgggt ctccgcccat
     2521 gcccgcaact cttccccggg agcagagggt caaaaccctg atctcatgaa catttggaaa
     2581 tgctgacggt cgatcaggtc gaagtcggcc gtgcgccggt acccggccga ttccatctcg
     2641 cgccggacct cctcagctgc agaaccatga cccaaaagac ccgagaagaa gccatgcggg
     2701 tggaaatcaa gaatcgccac ccggccgtct ggcttgagat atcgggtcaa tgatttgaag
     2761 tatgcgacac ggtcgtccat gtgatggtag gtgtcgcaac tgaacaccag gtccaccggt
     2821 tcaggtaatc ttgcgtccgt cggttcggcg tgaaccggca cgacattcgt gatgtgcctc
     2881 atctgcattt ccttcgtgat gatcttcaag cctgtctcat cgatatccac tgcgtacact
     2941 cgtccttgag gcccggcagc gctcgccaag tgccaggtga aatatccccc gcccgcacct
     3001 acatcagcga tccgggatcc cggagtaatg ccgagtctct cgaccacgtc tttcggtttt
     3061 tgccaagcat cccgagaggg atcgttcatg tgttgatagg cgaggtgggc gcaacccgac
     3121 agccataggc acgagaagag gaacagaccg atgcggctct gtagtgatgc agtgattctc
     3181 atgagatgga atggtgtgtg tgacgaagct aggccctcag tcactcatga gtcaagcgca
     3241 gccaactgta gaatgacaag ggggctcccc agtgttcaag acggtctgtc tttcgtatag
     3301 ggacagactg tctctgactc taagatggct gcgatccagt tttttcgcag gaacctggac
     3361 gaactgttct gtcgtactcg attccactga aaaccgatga tcataactcc tcacaatcat
     3421 tacgtttaga tttctggcaa aaagattgcg gcgcaacact gaatcagacg catcgtggcg
     3481 tctgagctta ttttctttta aggaggtact atggcgaaat tgagccgata tagagtcgca
     3541 atggtagcgc tatcggcgct gatagcatct ctgggacttt ttgcgttatc cggtcacgcc
     3601 gctgactcgc gcgcgatcag cgacggctca aaggtcaccc tcctctacca gattacggtg
     3661 ccgggggatg agcggttcga ggtccgggat cttagccagt tcgtacaggg acaacatcag
     3721 atgctacctg cggtggagca ggcggtcacg gggatgcacc ggggcgagaa gacggaagtc
     3781 aaactcagcg aggaccaagc gtttggcgcc tatgattcga gcaagaaaac catggtgcct
     3841 aagaaagaat tgcccgcagg agccaagacc ggcgatatta tagaagaccg taagaccggt
     3901 aagcaggcca ccattactca gatgtctgac actgacgcgg tcatggacta taaccatccc
     3961 ctcgcgggca agccgctcct cgtcagcttg accattttgg aagtcgagta atctgtcacg
     4021 cgacacccag ctcgcgatag ccggtcattg gccgtcgcga gcggtgtctt gctcaccaaa
     4081 tccgctacct tgaatgcaga ctggccggcg ctcccaagcc gacagtcagt ggagtatact
     4141 gtggtgatcg ggggcacgat tcatgagcct ctattctctg attgtcgtat tctccctcct
     4201 cacttcgctg ctcacttcgg cggctcagag ttcggccggc aatcaaatct accgctataa
     4261 agacgactcg ggcacggtga ccttcaccac agagttccag tcgattcccg agaaataccg
     4321 ccgcgaggcc gttcctctgg cgaccaatcc tccgccgcct cctgaacgaa ggcatgagcc
     4381 ttcagtgcgc atcgtcacat caagcggtga gtttcgcatg gggaattatg acacaaggac
     4441 ggacgccacc cgcatggcca tcgaggctgc caaacgcgat gcgctcgagc aagtggcgac
     4501 ctatttggag agtgtcactg aagtcaaaga tctcgacgtc acgcgcgatg aaattcggac
     4561 atacacagcc ggcgtcgtga cggtcctcaa tcagcagacg agcacacgtc tcgaaaaggg
     4621 cgtcgccatc attcacgtcg atctcaccgt gcaagccgat caacaggaag tcattcaagc
     4681 catcaccgcc ctacgggaaa acgagagcgc caaagtgcaa ctcgcctcgc tccgaacgga
     4741 aacggaacaa cttcgcctgc aactggacgc agcgaatcag gccttggcca cggccctgac
     4801 agcggaacag gtgcaagccc tcacgctgga acgacagcaa ttgctcaatc agatacaagc
     4861 cgacgcactg gtcgcccgag cgctgaccga ttttgcctac ctgacgccga tcaaagtgcg
     4921 gcaagtcaac gacctgctgc tacaggccag gcaactccat cccgcaaacc agcatctgca
     4981 agccgtcgaa caagcccttg ccacgaagac aggcccctcg gctttgccgc ttgtggttcc
     5041 atcgccagga aacgcgccga gcgcatcccc ggacgtggtc cccgctcagc ggtcaccctt
     5101 ggtcgcgccg ctgattgaat ctccccttgc aacgccgctt cctccgtcac cattggccac
     5161 accgttgccg ccctctccac ttgccgcccc tttgccgccg tcaccactgg ccgcacccct
     5221 tccaccctca ccgctggcga agccactcgg cccctcgcct cttgccacac cacttgcgcc
     5281 atcgccgtta gcggcaccac tccggtagcc gacctgtcgt gagatttcca cgtcgccggt
     5341 gtttcctccg gacgcatctc cgcttcgcac ggcggaacgt acacattccc tctggcggat
     5401 gatcgtcgcc aaatcattcc ctagtgcttc ttcaaatacg agtttgttac cacagagatc
     5461 ttcgttgaaa ggaggtcggt gtggcaccgc ggcaattgga gggaatgcgg gtcgcgatag
     5521 tggtcaccga tgatttctaa caatccgaat tgaccacacc gagaaaggcg ctcgaagaag
     5581 ccggcgctca ggcgcttatc gtctcgcccc catctctttt cggattatca gggtagtcga
     5641 accgcaaaag ccgcgtgatg attcctcgcc actcacttgc gttcaaccgc gcacgacgtt
     5701 ctcaatgatt cctcagccag cagcttcttg tcgtcaacca tcttcatgag cagcttggcg
     5761 gcagtaaaga gatcgttctg ctcctcttcg gcgatccaga atttcgtctt gatctgcgtc
     5821 actcgttcgg ccagtccatc cgactcttcc ccggcataca tgagctgtcg caatgcaatg
     5881 tggcggactt cgcagccgca ctgccgtggg tcattcaccg gcagccgccc acgcatctcc
     5941 tgatcctgtc tcgcgatggg aatgcccatg cctgccagca aggaccgcct ggactcgagg
     6001 agcatcgcat ccatagcatc caacgcattg gtatcgacga agtagtcaat caacgagcgg
     6061 ctggactgcc ccacgttgag ct
//
LOCUS       DASSCQ010000010        13649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_707052, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000010 DASSCQ010000000
VERSION     DASSCQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707052"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2331)
                     /gene="metH"
                     /locus_tag="VFH05_00765"
     CDS             complement(<1..2331)
                     /gene="metH"
                     /locus_tag="VFH05_00765"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET6676776.1"
                     /translation="MTPVELLLKERILILDGAMGTMIQQRRLDEAAFRGERFRDWKKD
                     VKGHNDLLNLTQPAIIEEIHRQYLEAGADIVETNTFNSQAISLSDYLMPQLAYELSRA
                     GAECARRAVVQVQSAQPKRRCFVAGAIGPTTKTSSISTDVNNPAARGATFDELVAAYA
                     DQVRGLLDGGADLLLVETIFDTLNAKAAFFAIQQLFATGAPRVPVMASVTFIQAGSDR
                     GVTGQTVEAFWNSISHVPLLSVGMNCALGPKEMRPLIEELSRIAPVYVSAHPNAGLPN
                     PLLPTGFPETPDTLAPQLREWAQNGWLNIVGGCCGTTPAHIKLLAESVRGIQPRPIPT
                     VEPYTRLSGLEALTIRPDANFVNIGERTNVTGSPAFAKLILQGNYETALAVARQQVEG
                     GAQIIDVNMDEGMLDSKAAMERFLRLIASEPDICKVPVMVDSSKWEILETGLKNIQGK
                     GVVNSISLKEGVPKFIEQATLVHRYGAAVVVMAFDEKGQADTLERKIEICTRSYKILT
                     EQVGFKPEDIIFDPNILTVATGIEEHNNYAVNFIEATRWIKQHLPGAKVSGGISNISF
                     SFRGNNVVREAMHAAFLYHAIQAGLDMGIVNAGQLAVYEQIPKDLLELVEDVLFNRRP
                     DATERLVTFAETVKRQDKAVVKDDEWRSGTVEERLSHALVKGITDYIDQDTEEARQKY
                     PKPLSVIEGPLMAGMNIVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFMEEEKRRVG
                     GYRSQGKVLLATVKGDVHDIGKNIVGVVLGCNNYEVI"
     gene            2523..3260
                     /locus_tag="VFH05_00770"
     CDS             2523..3260
                     /locus_tag="VFH05_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676777.1"
                     /translation="MAGLCLLPASLLSAPYFEDGFLGLTQKELHEKMGLPQAVRDRKS
                     ALRVFSYYAITDWDKYFRKLVSPENGEDVYTFKRDGVDVRYSFSYAVDPNEDSENRTL
                     YVRLVDIEFSPAVPISQVPSLVPEFRPSIDPASPSFRSNIWVLLFKGPASPQARFIVK
                     EAGKDSLDWTLAYQLFSLQGLPDPLTTKSAVDRLEISTQSIDLVKRRQKHTHDPIMNP
                     YSSEFSQQQSPPRPAQAKKIPVPKYAD"
     gene            complement(3293..3808)
                     /locus_tag="VFH05_00775"
     CDS             complement(3293..3808)
                     /locus_tag="VFH05_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676778.1"
                     /translation="MSILLPLRPLSFLLLVCFGTSWATDIQAKTLYTYTDQSGTRVIT
                     DNYNKIPPSYRSRATAVEQESEGYTAELERVGEIVVSSKGFVFNVPGMSIQQSRIITY
                     AGVIALCCVVAMNLSRSQAIRLLALWCLVLTGIGAPVLVYVADDGAAAVMKNKAAQIQ
                     QKQQDRLSQAQ"
     gene            3920..4363
                     /locus_tag="VFH05_00780"
     CDS             3920..4363
                     /locus_tag="VFH05_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676779.1"
                     /translation="MTSPESSSATTSDPHEPVSFIRRRSVRLIIYGGLIAFVVIMILV
                     PLIAKLRDPNFEKHIAEHRVMVGMSKEQVLQSWGGPQTINTSFTKDGIRQEEWIFEDW
                     ESAAVVKHRYLYFEEGTLIGGWFQGSGERLPTQSPSNPHNPKQTG"
     gene            complement(4367..4609)
                     /locus_tag="VFH05_00785"
     CDS             complement(4367..4609)
                     /locus_tag="VFH05_00785"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET6676780.1"
                     /translation="MNLVRLAEPYNVGELVLLQSLLEGSGIAYLIRHANVSSLYPGVP
                     GLNSHVMVEERDYPRAEAILQRLRLDVREVTGDIGR"
     gene            complement(4703..4987)
                     /locus_tag="VFH05_00790"
     CDS             complement(4703..4987)
                     /locus_tag="VFH05_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676781.1"
                     /translation="MKQVTVQNPDEILSLLAEVALRGSGFVTECLLDYVLDEGFTEPI
                     YLNASGEDPDAYFKGQSPAWAVYQVREWKRVLTISGGPGRQRRVQITETP"
     gene            5023..5790
                     /locus_tag="VFH05_00795"
     CDS             5023..5790
                     /locus_tag="VFH05_00795"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET6676782.1"
                     /translation="MRKATRMTRPQTNSRFQEELLRIMDRKHHWAWPSFADGTVSVEQ
                     LTRHFQQEYAVYVRDFPVMLARIHGHNPPAAVRRMLAENIYEEDTGGLSLGKSHPELF
                     LQMMAGLGLPAKSFERITLLPAARRYRAWLDRASYHRQWIVGAAALTVFVEGSVKDRK
                     ELDEPSKPKTAEEIESIVQQHPLVRHYGLSPDSMDLIRSHQMVEAGHRHDAYKMVVSY
                     ATTRAQQQAVLSCLKTCLRLWSAYRDGVARACRLRKP"
     gene            5765..6676
                     /locus_tag="VFH05_00800"
     CDS             5765..6676
                     /locus_tag="VFH05_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET6676783.1"
                     /translation="MPVVSESPDVRHSAGWFILCCVTLMMSSEWSFGFSDSTMVFIPA
                     GEFTMGTPAGTDGLADEHPQRFVFLSSFYIDRYELSNAKYFAFVSATHHRIPENANPA
                     STLWINGAPMRGIEQHPVVNVSWTDAEAYCRWAGKRLPTEAEWEKAARGTDGRRYPWG
                     NQWDIALANSASFWAGRTIEFDSGADWDAFWVKGEGAQIAKSKGLNGEVLTLPVGSFP
                     TNASAYGLLDMAGNVAEWVQDWYDPNYYKYAPLDNPVGPDRGAIKSMRGGSWLKPAVS
                     LRTSDRDWGTLDSRPSGTGFRCATDAF"
     gene            complement(6678..7343)
                     /locus_tag="VFH05_00805"
     CDS             complement(6678..7343)
                     /locus_tag="VFH05_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676784.1"
                     /translation="MRIPFHILTLAGLLLMPAEGWNGVTDEAGVPPRLSLQELEGMLP
                     PGTRLMVHPAAIEKFLAELDGAPPDWETLHGRGHQDPGHDDRLFAFNRERDARRGGHE
                     ALGQAVAFLWTGELSRYDPGSDGFPIALGPKLVKTTWGMVRFKPEDVPANLNVTTDVI
                     RREQWRRQIERGHPVEIDVVMSGKLIPDESIVYDFSHDEEGLGLIMPFVRVERVDFVL
                     INP"
     gene            complement(7354..7674)
                     /locus_tag="VFH05_00810"
     CDS             complement(7354..7674)
                     /locus_tag="VFH05_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676785.1"
                     /translation="MSDLDLLLQRTIAAISQSDPLIKLLEQVRLGRMQAGDPGLRAIT
                     ESWLAEYRKAIDSPGLTRQSLLRIDPAPRLTVLMEQGILPPNHEGVRRLLADFTQALS
                     RAST"
     gene            7976..8248
                     /locus_tag="VFH05_00815"
     CDS             7976..8248
                     /locus_tag="VFH05_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676786.1"
                     /translation="MYQEEKTFTMRFSLEASFPDDYDGEDDRHAWIHEWESQIKPHVL
                     KQVFESLRQHPAWSSHVRNRGIAPTDEIEIVVSKDFSKPVPFSIKS"
     gene            8245..9378
                     /gene="hemW"
                     /locus_tag="VFH05_00820"
     CDS             8245..9378
                     /gene="hemW"
                     /locus_tag="VFH05_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET6676787.1"
                     /translation="MTPALGLYLHIPFCRQRCDYCAFYLEVHREPAAEAFCRALETEI
                     ALYAAEPCFKGRQLASLYFGGGTPTVLKTDQLAGLLDSVQRSFPLSPACEITVEAHPA
                     TVTTRDLAALRDRGVTRISFGAESLADDELANLGRPGTQAETIAAVMSARSGGFTDFN
                     LDLMYGLPGQTLESWERTLHRCIHLGPTHVSCYALTVEEGTRLAHEVRRAQKQEPDES
                     LQIEMDRSAQDILGCAGYEQYEISNYARPGFQCRHNLLYWTQGEYLGVGPSAQSLVGN
                     VRFGNVANLTRYKASLTESRFPVQERTLLTPQEQLRDAVIFGLRLRQGVPTSQLRAHV
                     ENYGHVETWRELSADQLIEDVCGRSRLSARGRLFADTIAERLF"
     gene            complement(9433..9792)
                     /locus_tag="VFH05_00825"
     CDS             complement(9433..9792)
                     /locus_tag="VFH05_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676788.1"
                     /translation="MLDAKVWWPLFPLLLLLVIVCLTWALVIAVRGHADTRAKWLQLG
                     AFACYVIAAVSAVASERGAVSANLHRPFSLATQLCLLLALVHHWKSPQRPLRWLNAAA
                     WGGILADTALHVLMVRH"
     gene            9896..10081
                     /locus_tag="VFH05_00830"
     CDS             9896..10081
                     /locus_tag="VFH05_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676789.1"
                     /translation="MDPAKKHRKQARTAQVPSSEHSPPSRKSFGEETEEDRIKALADA
                     EKKNLWDATEVIDMDTF"
     gene            complement(10101..11177)
                     /gene="cax"
                     /locus_tag="VFH05_00835"
     CDS             complement(10101..11177)
                     /gene="cax"
                     /locus_tag="VFH05_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HET6676790.1"
                     /translation="MNSSFSMSWLDLLLVFVPVTVVLAFIQPGHLGVFISAALAIIPL
                     AGLLGRATEHLSAHVGAGIGGLLNASLGNAAELIIALVALRGGLHDVVKASLTGSIIG
                     NILLVLGASMLAGGLKHERQKFNQTAAGMGASLLLLAAVGLIIPALFHLTAADRGIAV
                     ERELSLEISVVLFAIYVMSLVFSLRTHRHVYSGGGQETSHVGERLWSRGRAMTVLATV
                     TVLIAVMSELLVGALDHAAETLGLTQIFVGVILVALVGNAAEHSTAVMVAMKNNMDLA
                     LSIAVGSSIQIALLVAPILVFASYLFGAPLDLIFTPFEVAAVTMSVLIVGFVSLDGES
                     HWMEGVMLVGVYMMLAIAFYFLPA"
     gene            11311..11607
                     /locus_tag="VFH05_00840"
     CDS             11311..11607
                     /locus_tag="VFH05_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET6676791.1"
                     /translation="MAESPTPVATPLTTETPSTGTGDGLEARVVVYNCECHTYQQVIA
                     LFCQCIPGMTPSKAFELAWKIDHQGEAVVFTGEWKQAEEIAEKLAGGGLRVAVQ"
     gene            complement(11615..11947)
                     /locus_tag="VFH05_00845"
     CDS             complement(11615..11947)
                     /locus_tag="VFH05_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676792.1"
                     /translation="MERRAASHTEEEEMNQRRRLLNAAKKGDQKAIAKLFELYQVRVY
                     NNEMVSKLNRTAGTHKLQAEVAQAKAAAKGGGSSRKSMPKKSVPSSSRQHGKARPARS
                     DKKVHRKK"
     gene            12137..12811
                     /locus_tag="VFH05_00850"
     CDS             12137..12811
                     /locus_tag="VFH05_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HET6676793.1"
                     /translation="MDAHKPDQPTVADDPQAREVLRRAFENTARWPKDFKGFTADLSV
                     NLNGRELHGPVIVKGPREVSVQLADGDLQKWAQDQLAMIAVHRGPRSFEESDGKYSLT
                     MEEDGHPLGTRLDIHGSNSFYRVKDGRITQINRTMAHPGMPAFAFTINVEDSAITQDK
                     KYLTTRYTVYYFSPEDGRLKNVESFTDTHVRVGASDLPASRRIIAFENGSVVVKNLTF
                     KNHQLL"
     gene            12817..13245
                     /locus_tag="VFH05_00855"
     CDS             12817..13245
                     /locus_tag="VFH05_00855"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HET6676794.1"
                     /translation="MDLRKDFPRSMRATAGGYAHLARMIDKCRAVLSGTQGEYIYPCP
                     LDRRLLDFVGITSDQFTAAVRSHQSDEGVVAWLREVATPRSTGELEKWNKDLLASGPS
                     SPESAERFKHDRDAVDPSRTDIAAWADLQDLEEGRAVPRR"
     gene            complement(13299..>13649)
                     /locus_tag="VFH05_00860"
     CDS             complement(13299..>13649)
                     /locus_tag="VFH05_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676795.1"
                     /translation="MRHVNFGLPSGQAVARVMGVPVLTQAQLAELQPFGMDRSTPLWF
                     YILKEAQLMESGLRLGPVGGRIVGEVFIGLLQADRDSYLVAEANWKPVLPSATAGDFR
                     VTDLLKFAGVVPPL"
BASE COUNT         3055 a         3961 c         3761 g         2872 t
ORIGIN      
        1 aatcacctca taattgttgc agcccaggac aaccccaacg atattttttc caatgtcgtg
       61 gacgtctccc ttgaccgtcg ccagaaggac cttgccttgc gagcggtatc cacctacccg
      121 ccgcttctct tcctccatga acggcagcag ataagcaacc gcctttttca tcacgcgggc
      181 gcttttgacc acttggggaa gaaacatttt gccggaaccg aagagatcgc ccacgatgtt
      241 catgccggcc atgagcgggc cttcgatcac cgacaagggc ttcggatatt tctgacgggc
      301 ctcctctgtg tcctggtcaa tgtagtcggt gatgcccttc accaaggcat gcgagaggcg
      361 ttcttcgacc gtcccgctgc gccactcgtc atccttcacg acggccttgt cctgacgctt
      421 gaccgtttcc gcaaaggtca cgagacgttc ggtggcatcc ggacggcgat tgaaaaggac
      481 gtcctcgacg agttcgagta aatcttttgg aatttgctca tagacggcca attgaccggc
      541 attcacgatg cccatgtcga ggcctgcctg gatggcatgg tagagaaagg ccgcgtgcat
      601 cgcttcgcgg accacgttat ttccccggaa ggaaaacgag atattgctga tgcccccgct
      661 gacctttgct ccgggaagat gctgcttgat ccacctcgtg gcctcgatga aattgaccgc
      721 gtagttgttg tgctcctcga tgccggtggc gacggtcaga atgttgggat cgaaaataat
      781 gtcttccggt ttgaaaccaa cctgttccgt caggatcttg taggatctgg tgcaaatctc
      841 gattttgcgc tccagggtgt cagcctgtcc tttttcatcg aatgccatca cgacgacggc
      901 ggctccatac cggtggacca acgtcgcctg ttcgatgaat ttcggcacgc cttctttcaa
      961 gctgatgctg ttgacgaccc ccttgccctg tatgtttttc aggccggtct ccaggatttc
     1021 ccacttggag ctgtcaacca tgaccggaac tttgcagata tccggctcgg aggcgatcag
     1081 tctcaaaaac cgttccatgg cggcctttga atccagcatg ccctcatcca tgttgacatc
     1141 gatgatttgg gcgcctccct ctacctgttg tcgtgccacc gccaaggccg tttcgtaatt
     1201 gccctggaga atgagcttgg caaacgccgg tgacccggtg acattcgtcc gctctccgat
     1261 attcacaaaa ttggcatcag gacggattgt gagtgcctcc agtccgctca agcgggtata
     1321 aggctccact gtcggaattg gacgcggctg gatcccgcga accgattcgg ccaggagctt
     1381 gatgtgcgcg ggtgtggtcc cgcagcaacc gccgacaatg ttcagccatc cgttctgtgc
     1441 ccattcccgc aactgcgggg ccagcgtatc gggcgtctcg ggaaagcctg tcggcagcaa
     1501 cggattcggc agaccggcat tcggatgggc gctgacatag acgggcgcaa ttcgtgatag
     1561 ctcctcaatg agcgggcgca tctctttcgg acccagggcg cagttcatcc cgacactcaa
     1621 gagcggcacg tgtgagatag agttccaaaa cgcctccacc gtctgacccg ttacaccacg
     1681 atcgctgccc gcttgaatga atgtcaccga ggccatgacc ggcacgcgtg gggctcctgt
     1741 tgcgaagagt tgctggatgg caaagaaggc ggcttttgca ttcagcgtgt caaagatggt
     1801 ttccaccaac aacaggtcgg ctccgccgtc caacagaccc cgcacctggt cggcataggc
     1861 ggccaccagt tcgtcgaagg tcgctccacg cgccgccgga ttgttgacgt ccgtcgaaat
     1921 ggaagatgtc ttggtggtcg gtccaatcgc accagccaca aaacaccgtc gcttcggttg
     1981 agcactctgg acttggacga cggcccgccg ggcgcattcg gcaccggccc ttgagagttc
     2041 ataggccaac tgtggcatca ggtaatccga aagggagatc gcctgtgaat taaaggtatt
     2101 cgtttcgacg atatcggcgc ccgcttccag atactgacga tggatttctt cgatgatcgc
     2161 cggctgcgtc agattgagca gatcattgtg tccttttacg tccttcttcc agtcacggaa
     2221 ccgctcacct ctaaaggcgg cctcgtccag cctccgctgc tggatcatcg ttcccatggc
     2281 tccatcaagg atcaggatgc gttctttaag tagcagctca acaggcgtca cgtcgcgttc
     2341 cttccctctg taagatcgga atcgtgtcat catactgatt caggttccac cgggcaacca
     2401 atcggacaac tccaccttga cagaggtcga gcctgccgat acgatggcgc atcggccgga
     2461 gacgaggcgc tttgctcctg cagaaccatg tacaacgctt cgccacgtgc gctttcgggg
     2521 tcatggccgg cctctgccta ctccccgctt cgctgctctc ggccccctat ttcgaagacg
     2581 gtttcctggg tctcacgcaa aaggaattgc atgagaagat gggactgcct caggctgtcc
     2641 gcgaccgcaa atccgctctg cgggtcttca gttactacgc gatcaccgac tgggataaat
     2701 attttagaaa gttggtgtca cccgagaacg gcgaggacgt gtacacattc aagcgggatg
     2761 gcgtcgacgt ccgctattcc ttcagttatg cggtggaccc caatgaggat agcgaaaacc
     2821 ggacgttata tgttcgcctg gttgacatcg aattttcgcc agccgtaccc atcagccagg
     2881 ttccgtctct tgttcctgaa ttccggcctt ccattgatcc ggcatcgcct tcatttcgat
     2941 cgaatatctg ggtccttctc tttaaaggcc ccgcctctcc gcaggcacgg tttatcgtca
     3001 aggaagctgg caaggattcg cttgactgga cactcgccta tcagttgttt tcgctgcaag
     3061 gtcttcccga ccctttgacg acaaagtcgg cggttgaccg cctcgaaatc agtacgcaaa
     3121 gcatcgatct tgtgaaacgc cggcaaaaac acacccacga tcccatcatg aatccctatt
     3181 catctgagtt ctcccaacag cagtctcctc cgcggccggc tcaagccaaa aaaatccccg
     3241 tcccgaagta tgcggactga agagtcgcgt gaaggaaggg ctgtgacggc gcttactggg
     3301 cttgcgagag gcggtcctgc tgcttctgtt ggatctgtgc tgctttgttt ttcatgacgg
     3361 cggctgcacc gtcgtccgcc acatagacca ggaccggcgc gccgatccct gtgaggacga
     3421 gacaccacag cgccagcaag cggatggctt ggctgcggct gagattcatc gcaaccacac
     3481 aacacagggc gatgacgccg gcatacgtga tgattcttga ctgttgaatt gacatgccag
     3541 gcacgttaaa aacaaaccct tttgatgaga cgactatttc cccgacccgc tcgagttcgg
     3601 ccgtgtagcc ctccgactct tgttctactg cggtcgccct gctgcgatat gagggaggga
     3661 ttttattgta gttgtcggtg atcacgcgcg ttccgctttg gtcggtatag gtgtagagcg
     3721 tcttcgcctg tatgtcggtc gcccatgaag tgccgaaaca cacaaggaga agaaaagaca
     3781 gagggcgaag gggcagaaga atgctcatgc atgaagtata gctaatccat ctatcacgtt
     3841 gattatattg tgttaatcat tactgaacat tgacgttcgt ccgtctgatt gctaccatgc
     3901 gtccgcctca aacacagcca tgacatcgcc agagtccagc tccgctacca cttcggatcc
     3961 gcacgaaccg gtctcattta tccggcgcag atccgtccgc ctgatcattt atggagggtt
     4021 gatcgcattt gtcgtcatca tgattctcgt accactcata gcaaaattgc gcgatccgaa
     4081 ttttgagaaa cacattgccg aacaccgggt catggtgggc atgtcgaaag agcaagtgct
     4141 gcaatcatgg ggtggacctc aaacgatcaa taccagcttt acaaaggacg ggatccggca
     4201 ggaagaatgg atattcgaag actgggaaag cgcggcggtc gtcaagcacc ggtatctgta
     4261 cttcgaggaa gggacgttga tcggaggatg gtttcagggc tccggcgaac gattgcccac
     4321 acaatcaccg tcgaatcccc acaatccgaa gcagaccggc tagatgttat ctgccaatgt
     4381 cgccggtcac ttcacgcaca tcgagtctca agcgctgcaa aatcgcctcg gctcgcgggt
     4441 aatcccgttc ctccaccatg acatgagaat tcaatcccgg cacgcccgga tagagactac
     4501 tcacgttggc atgacgaatc aaatacgcga tcccgcttcc ttcaagcaga ctttgaagca
     4561 ggaccagttc gccgacatta tagggttcgg ctagacgaac cagattcatg tcatccatcc
     4621 ttggtacatg attcctgtat ccaagagcat agcaccggat cgttgctttt tcgacgcgac
     4681 actcctacac tgagttcaag acttatggtg tctcagtgat ctggacccgc cgctgcctgc
     4741 cgggacctcc cgaaatagtc agcacgcgtt tccattcccg aacctgatac accgcccagg
     4801 ccggtgactg tcccttaaag taggcatccg gatcttctcc gctggcattc aggtaaatcg
     4861 gttcggtaaa tccctcgtct aagacgtagt cgagaaggca ttcggtgacg aaaccagatc
     4921 cgcgcaacgc gacctcggcc agcagcgaca ggatttcatc ggggttttga acggtcacct
     4981 gtttcacatg tcctccatcc ggcgaattgt aacgaggccc tgatgaggaa ggcaacccgc
     5041 atgacccgac cgcagaccaa cagccgcttt caagaagagc tcctgcgaat catggaccgc
     5101 aagcaccact gggcctggcc atcattcgcc gacggaacag tctcagtcga gcaattgacc
     5161 cgccattttc agcaagagta tgcggtctac gtgcgcgact ttccggtcat gctcgcacgg
     5221 attcatggcc acaaccctcc ggccgcggtc cggcggatgc tggctgagaa catttacgaa
     5281 gaagacaccg gaggtctttc actcgggaaa tcccatccgg aactgtttct tcagatgatg
     5341 gctggattgg gcctccccgc caaatccttt gagcggatca cgctccttcc cgccgcacgt
     5401 cgatatcgag cctggctcga ccgtgcttcc tatcatcggc agtggatcgt cggtgccgcc
     5461 gcgctgaccg tctttgtgga aggcagcgtg aaggaccgga aggagttgga cgagccttcc
     5521 aaacccaaga ccgcggaaga aattgagtcg atcgtccaac aacacccgct ggtccgccat
     5581 tatggcctct cgcctgattc catggatctc atccgttctc atcagatggt cgaggccggg
     5641 catcgacatg acgcctacaa gatggtcgtg agctacgcga cgacgcgtgc gcaacaacaa
     5701 gcggtcctga gttgtctgaa gacctgcctg agattgtgga gtgcctaccg cgacggggtc
     5761 gctcgtgcct gtcgtctccg aaagccctga cgtgcggcac agcgccggct ggtttattct
     5821 gtgctgcgtc accctcatga tgagctccga atggagcttc ggcttctcag atagcaccat
     5881 ggtattcatc cccgccggtg aatttacgat gggaacgccg gccggcacag atggactcgc
     5941 cgatgagcac ccacagcgtt ttgtgtttct ctcctccttt tacatagatc gttatgaact
     6001 ctcgaatgcc aaatatttcg cattcgtctc cgcgacacat caccgaatac ccgaaaacgc
     6061 caatccagca tcaaccttat ggattaacgg ggcgccgatg cgcgggatcg aacagcatcc
     6121 ggtggtgaat gtcagctgga ctgatgcgga agcctattgc cgatgggccg gcaagcgcct
     6181 tccgactgaa gcagaatggg aaaaagccgc gagaggaacg gatggccgtc gatatccctg
     6241 gggcaatcag tgggacatcg cgttggcgaa cagcgccagc ttttgggccg gacgcacaat
     6301 cgagtttgac agcggagcgg attgggacgc cttctgggtc aagggtgaag gcgcccagat
     6361 cgcgaagagc aaaggattga acggagaagt gctgaccctg ccggtcggga gttttcccac
     6421 caacgccagc gcatacggtc tcctcgacat ggctggcaat gtcgccgaat gggtgcagga
     6481 ttggtacgat cccaactatt acaaatacgc cccgttggat aaccccgttg gtcctgatcg
     6541 tggcgccatc aaatccatgc gcggaggctc gtggctcaag ccagcggtaa gtctgcgcac
     6601 aagcgatcgc gactggggca ccctggacag ccgcccgagt ggaacgggtt tccgctgcgc
     6661 aacagacgcc ttctgactta aggatttatc aagacaaaat ccacccgctc gactcgcacg
     6721 aagggcatga tcagccccaa gccctcttcg tcgtgagaaa aatcataaac aattgattcg
     6781 tcaggtatga gcttcccgct catcacgaca tcgatctcga ccgggtgccc ccgctcgatt
     6841 tggcgccgcc attgctcacg ccggatcacg tcggtcgtga cgttcagatt cgctggcacg
     6901 tcttcaggct tgaaacggac catcccccag gtagttttga cgagcttcgg gccaagggcg
     6961 atgggaaaac catcgctccc cggatcataa cgggaaagtt cgcccgtcca taggaaggcc
     7021 accgcctgtc ccagcgcctc gtggcctcca cgtcgcgcat cccgctccct gttgaacgca
     7081 aagagccggt catcatgacc tgggtcctgg tgtccgcgac cgtgcagcgt ttcccagtcc
     7141 ggcggtgcgc catccaattc tgccaaaaat ttctcaatgg cagcgggatg gaccatgagc
     7201 cgggtgcctg gaggaagcat gccctctagt tcttggaggg agaggcgcgg aggcacaccg
     7261 gcttcgtcgg tcacaccatt ccatccttca gccggcatga gcaataaccc cgccaaggtc
     7321 aggatgtgaa acgggatcct catcgggtat cgatcaggtc gatgcacgac tcagggcctg
     7381 ggtgaaatcg gcaagcaatc tcctcacccc ctcatggttc ggtggcaaaa tcccttgctc
     7441 catgagcacg gtcagtcgag gcgcaggatc gatgcgaagc aacgactgtc gtgtcaatcc
     7501 tggtgagtcg atggctttgc ggtactctgc aagccatgat tctgtgatgg cacgcaggcc
     7561 gggatcgcca gcctgcatgc ggccaagccg tacttgttca agaagtttga tgaggggatc
     7621 ggattgcgaa atggccgcaa tagtccgctg gagcagcaga tcgaggtcgc tcattgctag
     7681 ttcatcgaac aggctcccgg tcctctcgga tctccgcagc tgccacaggc gcctcctccg
     7741 ccggacgggg gggcccagcc acccgtggcg ccgaccccaa atgaagaaaa ttgtttgtcg
     7801 agcgtggtgg cgcggcactc tggacaggcg gcctcgaccg acccttgaac caggagttca
     7861 aagcgatgat tgcattcacg gcagacatat tcgaagatag gcataatcgt gactctccgg
     7921 tggttgaacc cattatagaa gtgtcgcgtc gcgcttcgca acgcacgagg cttaaatgta
     7981 ccaagaagaa aaaaccttta ctatgcgctt cagtctggaa gcctccttcc ctgacgacta
     8041 cgacggcgaa gacgatcgcc atgcgtggat ccatgagtgg gaatcacaga tcaagccaca
     8101 tgttttaaag caggttttcg aatcgctcag acagcatccg gcctggtcat cccacgttcg
     8161 aaatcggggc attgcgccca cggacgaaat cgagatcgtc gtctcaaaag atttttccaa
     8221 gccggtcccg ttctcgataa agtcatgacg cctgcccttg gcctgtacct ccatattccc
     8281 ttttgccggc agcggtgtga ctactgcgcc ttctacctgg aggtgcaccg tgagccggct
     8341 gcagaagcct tttgccgagc gctcgaaacg gaaattgccc tgtatgcggc tgaaccatgt
     8401 ttcaaaggcc gtcagctcgc gtctctctac ttcggcggtg gaactccaac ggtcctcaag
     8461 accgaccagt tggcaggctt actcgactcc gtgcaacgct cgtttcccct gagtccggct
     8521 tgtgaaatta ccgtagaggc gcatccggca accgtcacga cacgcgacct tgctgcctta
     8581 cgagatcggg gggtgacaag gatcagcttt ggcgcggaat ccttggctga tgacgagctg
     8641 gcgaaccttg gccgtccagg cactcaagcc gaaacgatcg cggcggtgat gagcgcccgg
     8701 tcgggtggat ttaccgactt caatctcgat cttatgtacg gacttcctgg acagacattg
     8761 gagagttggg aacggacgct ccatcgctgc attcatctgg gcccgacgca cgtctcttgt
     8821 tacgcgctga cggtcgaaga ggggaccaga ctcgctcacg aggtgcgccg agcgcagaag
     8881 caggagcctg atgaatcgct gcagattgaa atggatcgtt cggcacaaga catactgggc
     8941 tgtgccggtt atgaacagta cgaaatctcc aactacgcca ggcccggttt ccaatgccgc
     9001 cataacctct tgtactggac acagggcgag tacctcggcg tcgggccaag cgcccaatcg
     9061 ctcgttggaa acgttcgttt cggcaatgtg gccaatctca cgcgctacaa ggcctcattg
     9121 acggaaagcc gcttccccgt gcaggagcgg acgctactga ccccccaaga acagctccgt
     9181 gacgcggtca tctttgggct gcggctgcgc cagggcgtcc cgacttctca attgagagct
     9241 catgtcgaaa actatggcca tgttgaaacg tggcgtgagc ttagtgcaga tcagttgatc
     9301 gaggatgtct gcggtcgcag ccgactatcg gcgagggggc gtctattcgc cgatacgatc
     9361 gctgaacgct tattttaacg gtgcggcggt gtgggcggtg tgccaatagg gatcaagcat
     9421 ccattgcccg aatcaatgtc tgaccatcag gacatgcaac gccgtatcag cgaggatgcc
     9481 tccccaagct gccgcattca accagcgtaa tggtcgttgc ggagacttcc agtggtgaac
     9541 caaggcgagc agcaggcaaa gttgcgtcgc cagtgaaaag gggcgatgaa ggttggctga
     9601 gacagcccca cgttcgctgg ccacagccga cactgcggcg atgacgtagc acgcaaaggc
     9661 gccgagttgc agccactttg cccgagtgtc agcgtgaccc ctgaccgcaa tgaccagcgc
     9721 ccacgtcaaa cagacgatga caagtaacaa caacagcggg aagagcggcc accacacttt
     9781 ggcgtccaac atgggcgcgg actgtaacaa gcctttccca ggaggtcaac gacagctcag
     9841 ccattgactt ttttcacccc taggagagag actaacacca cctatgccag tgaacatgga
     9901 tcccgcaaaa aaacatcgta aacaagcgcg taccgctcag gttccgtcct ctgagcactc
     9961 cccgccttcg cgcaagtcgt ttggcgagga aacggaggag gatcggatta aggcgctcgc
    10021 ggatgcggaa aagaagaacc tgtgggatgc aacagaggtc atcgacatgg atactttcta
    10081 attgtgtgct tctcgtcgtt tcaggcggga agaaagtaaa aggcaatcgc gagcatcata
    10141 tacactccca ccagcatgac accttccatc cagtgcgatt cgccatccaa cgaaacaaat
    10201 ccgacaatca agaccgacat cgtgactgca gcgacctcga atggcgtaaa aatcaaatcc
    10261 aacggcgcgc cgaagagata actcgcaaag accaaaatcg gcgcgacgag cagggcgatt
    10321 tgaatgctcg atccaacggc aatgcttaga gccaggtcca tgttgttttt catggcgacc
    10381 ataacggcgg tcgagtgttc ggcggcgttt ccgacgagcg cgacgaggat gacgcccaca
    10441 aagatttgcg ttaatccgag cgtttctgcg gcatgatcca acgccccgac cagcagttca
    10501 ctcatgacgg caatcagcac ggtgacggtt gccaggaccg tcatggctct tccgcggctc
    10561 cacaacctct cgccgacatg cgaggtctcc tgcccgcctc ccgaatagac gtgacgatga
    10621 gttctcaacg aaaacacgag gctcatcacg taaatcgcga ataagacgac ggatatctca
    10681 aggctgagtt cgcgttcgac agcgatgccg cgatccgctg cagtgagatg gaatagggcg
    10741 ggaatgatca gtcccacagc ggcaagcaag aggaggctcg cacccattcc cgctgccgtt
    10801 tgattgaact tttgccgctc gtgcttcagt ccgcctgcga gcattgatgc acccaggacc
    10861 agaagaatgt tgccgatgat cgatccggtc agcgaagctt tcacaacgtc gtgcaagcct
    10921 ccgcgtaacg ccaccagcgc aatgatcaac tctgctgcat ttccgagcga agcgttcaag
    10981 aggccgccga ttccagctcc aacatgagcc gacaaatgtt ccgtagcccg tcccagaaga
    11041 ccggcgagcg ggatgatggc aagcgccgcg gaaataaata cgccaagatg gcctggttga
    11101 atgaacgcga gcacgacggt gaccggaaca aaaaccaaca ggagatcgag ccaagacatt
    11161 gaaaacgagc tgttcatggc ggcgcacgtt aacatgagcg gccccctttg tcgacgaaca
    11221 ggcgctcctc gggacgtccg acgccacacc ggctgcgcac gcggaaaagg atcttaatcg
    11281 cacaggagaa ctctgttaga atcgccctcc atggccgaat cccccactcc tgttgcgaca
    11341 ccgctgacca cagaaactcc gtcgaccggg actggtgatg ggttagaagc ccgggtggtc
    11401 gtctataact gcgagtgtca cacttaccaa caagtcatcg cactgttttg ccaatgcatc
    11461 ccgggaatga cgccatccaa ggcgttcgag ctggcatgga aaatcgacca ccaaggagag
    11521 gcggtggtct ttacgggcga gtggaaacaa gcggaggaga tagccgagaa gctagccgga
    11581 ggaggcttac gcgtggccgt tcagtagccg gcctctattt tttcctgtgg accttcttgt
    11641 cggagcgggc tggtcttgct ttcccatgct gccggctcga cgaggggacg gattttttgg
    11701 gcatcgactt tcgggatgat cccccgccct ttgctgcagc cttggcctgc gccacctcag
    11761 cctgtagctt gtgcgtgcca gccgtgcggt tgagtttact caccatctcg ttattataga
    11821 cccgcacctg atacaactcg aaaagttttg cgatcgcctt ttgatcgcct tttttggcgg
    11881 cgttcaaaag ccggcgtcgc tgattcatct cttcttcttc ggtatgagaa gcggcccgtc
    11941 gttccatgcc atcctcgttc gaatgtctga tgcaatcgcc tctgatgatc ggcgaaggag
    12001 taaaccggcg aactatatca gaagcaaagg gcttttgaaa atgccggtgc caggacggcc
    12061 cagggctttt cctccattga gcgactcaaa acgaggcagt tataatgtca aagacttttc
    12121 tacatggagg gacattatgg acgcccacaa gccggatcaa ccgactgtag ccgacgaccc
    12181 tcaagcgcgt gaagtgctgc gccgcgcgtt tgaaaatacc gccaggtggc ccaaggactt
    12241 caaaggcttt accgccgatc tgagcgtgaa cctcaacgga agggaactcc acggtccggt
    12301 gatcgtgaaa ggtccccggg aagtgtccgt ccaactggcc gacggggacc ttcagaagtg
    12361 ggcgcaggac cagctcgcga tgatcgccgt ccatcgcggt ccaaggagtt ttgaagaatc
    12421 ggacggcaag tacagtctga ccatggaaga agacgggcat ccgctgggca cgaggctgga
    12481 catccacggc tcgaactcct tttatcgggt taaggacggc cgcattacgc aaatcaatcg
    12541 aacgatggcc caccccggca tgccggcctt tgcgttcacc atcaacgtgg aagacagcgc
    12601 gatcacccag gacaagaaat atctcacgac ccgatacacc gtgtattact tttcaccgga
    12661 agacggacgg ctgaaaaatg tcgagagctt caccgatacg cacgtgaggg tcggagcatc
    12721 cgatctgccg gccagccggc gcatcattgc gttcgaaaac ggaagcgtcg tcgtcaagaa
    12781 cctgaccttc aaaaatcacc agcttctctg acgtcgatgg atttgagaaa agactttcca
    12841 cgcagcatgc gggcgacagc gggcggttat gcgcatttag cgcgcatgat cgacaaatgt
    12901 cgagccgtac tcagtggaac ccagggtgaa tacatctacc cctgtcctct ggacagacgc
    12961 ctgttggatt ttgtcggaat cacgagtgat cagttcaccg cggccgtcag gtcgcatcag
    13021 tccgacgaag gcgttgtggc atggcttcgt gaggttgcca cgcctcgttc aaccggcgaa
    13081 ttggagaaat ggaacaagga tcttttggcc agcggaccaa gttcccctga gagcgctgag
    13141 cgattcaagc atgatcgtga tgccgttgac ccctcgcgaa ccgatattgc tgcatgggca
    13201 gatcttcaag atctggagga aggacgcgca gtgccgcgcc gataggaaat cccatcgtcc
    13261 cgcggcacct gaattgaggt acggagagac ggggtcggtt acagtggggg gaccacgccg
    13321 gcaaatttga gcaaatcagt gacgcggaag tcgccagcgg tcgcggaggg aaggacgggc
    13381 ttccagttgg cttcagcgac cagatacgag tcgcggtccg cctgtaacaa tccgataaat
    13441 acttcaccga caatccgacc gcccacgggt cccaaacgaa ggccggactc catgagttgt
    13501 gcctccttga gaatatagaa ccacagcggc gtactacgat ccataccgaa gggttgcagc
    13561 tcggccagtt gtgcttgtgt cagtaccggt actcccatca cacgagcgac ggcttgtccc
    13621 gaagggagac caaaattgac atgccgcat
//
LOCUS       DASSCQ010000011         4887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1585771, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000011 DASSCQ010000000
VERSION     DASSCQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585771"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFH05_00865"
     CDS             complement(<1..422)
                     /locus_tag="VFH05_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676796.1"
                     /translation="MRDLWTGHIFWIRNVVWDNAAHNAAARDAAEQEVVVNAKKIANT
                     ITPFYGEQASEKLFSLLAGHYGAVKEYSEATVAENRLQQDAALTHLASNADEIAVFLS
                     GANPHLPKDTVRGLIAAHGAHHVLQITQFKERDYVHEG"
     gene            complement(616..747)
                     /locus_tag="VFH05_00870"
     CDS             complement(616..747)
                     /locus_tag="VFH05_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676797.1"
                     /translation="MGDTIILIVSFPLIIAGMILTITWGMSAAGAGGQGGRKQTKEA"
     gene            complement(1017..1826)
                     /locus_tag="VFH05_00875"
     CDS             complement(1017..1826)
                     /locus_tag="VFH05_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676798.1"
                     /translation="MRHNMIWPLSVLVLAMGGTSAVSIGEAASHSEQVHPQLLPGDRI
                     LIGTVVEVRSGQTRIDTGEELPRFVPIEVREAKGLPDLKIGDGVEITVNDQNQLVDVH
                     LIGEASHHRVVEGQLIQSLVTRPDRAVLRTTGGKEESHAIRPTARSKMASVPVGVDAV
                     FLIDESDSIVDVTFGDMAAVSQAAKVWAKTTPLKGNFDRVIGTILTPLQNSRIEIRTE
                     DGEEQPYEVRPLAQLRVVRLSYGDAVILLVDNEGKVTGVAIPPRPSDSDRG"
     gene            complement(1880..2515)
                     /locus_tag="VFH05_00880"
     CDS             complement(1880..2515)
                     /locus_tag="VFH05_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HET6676799.1"
                     /translation="MNLKGLVHRELSEGVTEKELASAVGVSVRTLGDILADKLPEEPS
                     VWDAFARYFRIEADFLQAGGPPLPPGIFRLGESKHGVAVDPMRKVPLLNFHQVDQMLT
                     KHEPPGVVHAEAMLETDVTGNRTFAVTVKDDSMQPLFSEGEVIFVNPDLPSQSGHYVL
                     VDGDLNGALLRQLKNVGGEAVLHPLNRRYDDLPLSPRHRIWGRVVRLRKNL"
     gene            complement(2536..2781)
                     /locus_tag="VFH05_00885"
     CDS             complement(2536..2781)
                     /locus_tag="VFH05_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676800.1"
                     /translation="MDRLAEERSCIRCRGLLVSDWFYDLKNPVEYNVKVLRCVQCGHR
                     VDPTIVRNRIHPPVPDLEEGLGYQHSVSTEHVEDAAF"
     gene            complement(3014..4474)
                     /locus_tag="VFH05_00890"
     CDS             complement(3014..4474)
                     /locus_tag="VFH05_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6676801.1"
                     /translation="MKSLHGLIRQSALILIAGLLLVFSIVIYAGGDTLMRRFVDARLL
                     GLAETLAKIVEHNPDLMKAPMDDRVVSADVSRSEKEQLQVSGVTHSLRVFSPEGVLLW
                     NASDAEPQQPMSEQVFEQARLGHVVVETVESVDGTPVRHLFLPIFREGKVQYVLQAEA
                     SLRLYRETLKELVLLLSMGAATILLVAWLGSGWLAKKVLSPLAVLSTEAETMSEADLG
                     KRLILGSPYQEFRRLTQAFNSVMDRFQRSVESQRRSVDHAAHEMQTPLTVMQGHLEVT
                     LLKARTHEEYREALINNLEQVGRLISLTRSLLTLAKFTGDTPPVHLAPLALGALIPDL
                     LAELNILFDERRITLSFESQPVPNVYGDAQWIKQAVMNLLDNALRYTPSGGTVTVRLQ
                     TVDQWVAVIVEDTGHGIEPEHLPHLFERYYRTDWARAKDSGGTGLGLPIVKGIAAAHG
                     GTISVTSRVNEGSVFTLRLPIPIQETVPSPAALRVS"
     gene            complement(4471..>4887)
                     /locus_tag="VFH05_00895"
     CDS             complement(4471..>4887)
                     /locus_tag="VFH05_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6676802.1"
                     /translation="AFDIGADQFLTKPFAFAELLARVRSLLRRGGAQQPTRLTAADLH
                     LDPASRKVTRSGKDIILTNKEYALLEFLLRNKNRVLTRTAIIEHVWDISYDPMTNIVD
                     AHIRALRSKIDREFSPPLIVTVRGAGYMLEEPKPIE"
BASE COUNT         1077 a         1408 c         1276 g         1126 t
ORIGIN      
        1 tccccttcgt gcacataatc cctctccttg aactgcgtga tctgaaggac atggtgcgct
       61 ccatgtgcag caatcagacc tcgaacggta tcctttggaa ggtggggatt agctccgctg
      121 agaaacacgg cgatttcgtc cgcattcgaa gccaagtggg tcagtgcggc atcttgctgt
      181 agcctgtttt ctgcgacggt cgcttccgaa tactccttca ctgcaccata atggccggcc
      241 agtaaactaa agagtttctc ggacgcctgt tcgccataaa acggagtgat ggtatttgca
      301 atcttcttgg cattgaccac cacctcctgc tcggcagcat cccgggccgc agcattgtgc
      361 gccgcattgt cccacacgac attgcgaatc cagaaaatat gtcccgtcca cagatcgcgc
      421 atcgctgctt tcgtgtcagc gacgtggacc ggattggctt ctccaactgc ccgcgtgtcg
      481 tgtgcacagc ccgccaggcc aacgaccgcg agtgctagtg ctgcgataga gtgatgtatc
      541 aggctcataa accctccctt tatattggac ctcattgata tggggcagcg gggttctcct
      601 agaaggtgtc ggttctcaag cttctttggt ctgcttgcgc ccgccctgcc cgcccgcacc
      661 tgcagcgctc atgccccacg taatcgtcag aatcatccct gcaatgataa gcggaaacga
      721 cacgatcaga attatggtat cgcccatggt gcatctccct tcctcggatt cgaacgccta
      781 tctgcattgt tcactgtcaa cgtagcaacc taagatgaac tggccgtgaa cggacgatga
      841 agataccttc atcttcgaag cgtcttgctc gaaaatcggt ggttcgaggg ctctaagagc
      901 ctatagacga attggtatgc cgtaaggagc gtcggctcgt gatgggatca agcgaggcag
      961 gactctctga ggtcatcttc gctgcgacac atgcgcaaga cgtcacgccg taccctctat
     1021 ccccggtcgc tgtcgcttgg ccttggagga atcgcaacgc cggtgacttt gccttcattg
     1081 tccaccaaca gtatcaccgc atcgccatag gacagtctca ccaccctcag ttgagccagc
     1141 ggacgcacct catacggttg ctcttcacca tcttcggttc ggatctcaat gcgactgttc
     1201 tgcaatggcg tcaggatcgt cccaatcact ctatcgaaat tccccttcag gggtgttgtc
     1261 ttcgcccaga cctttgctgc ctgactcacc gcggccatat cgccgaaggt cacatcgaca
     1321 atgctgtctg attcatcgat gagaaagacg gcgtctacac caacgggtac agaggccatt
     1381 ttgcttcgtg cggtcggacg gatagcgtgc gactcttctt tgcctccggt cgtgcgcaga
     1441 accgctcgat caggcctggt caccagcgat tgaatgagtt ggccttcaac gacgcgatgg
     1501 tggctcgcct caccgatcag atgcacatca accagctgat tctgatcatt gacggtgatt
     1561 tctactccgt ctcctatttt caagtccggc aaccccttgg cctctcgcac ttcaattgga
     1621 acaaagcgcg gcagctcttc accagtatca atccgtgtct gaccgcttcg tacttccact
     1681 acggtgccta tgagaatgcg gtccccggga agtagctgag ggtgaacctg ttcagaatgg
     1741 gaggcagcct cgccgattga gaccgcagag gttcctccca ttgcgagtac taacacactg
     1801 agtggccata tcatattgtg tctcacgctt actccttccc aagtctggtt tgtgactttc
     1861 tccatgtatt cgataggcct caaagattct tccgcaaacg cacgaccctt ccccaaatgc
     1921 gatgccgggg gcttagcggg agatcgtcgt accgccgatt gagcggatga agaactgcct
     1981 ccccgccgac attcttgagt tgccgcaaca gtgccccatt caagtcgccg tccaccagga
     2041 cgtaatgacc ggactgactc gggagatcgg gattcacgaa aatcacttcc ccttcgctaa
     2101 agaggggctg catggagtcg tctttgaccg tcacggcaaa cgtgcgattt cccgtaacat
     2161 ctgtctcgag catcgcttcc gcatggacga caccgggtgg ttcatgtttc gtcaacattt
     2221 gatcgacctg atggaaattc aagagcggta ctttcctcat cggatcgacc gcaacgccat
     2281 gcttgctctc tcctagtctg aatatccctg gaggaagtgg tggaccgccg gcttggagaa
     2341 aatcagcctc gatccgaaaa taccgcgcaa aggcgtccca gactgacggc tcttcgggta
     2401 gcttgtcggc taaaatatcg ccgagcgtac gcacggagac cccaaccgca gaggccaatt
     2461 ccttttcggt gactccctcg ctgagctctc gatgtacaag acccttcaga ttcacaacgt
     2521 cctccccggg cgactttaga atgcggcgtc ctccacatgc tctgtgctta ccgagtgctg
     2581 gtacccaagt ccttcctcca gatctgggac gggcggatgg atacggttcc gcacaatcgt
     2641 tgggtcaacc cgatgcccgc attgcacaca acgcaacact ttcacgttat attcgaccgg
     2701 attctttaag tcatagaacc agtcgctcac gagcaggccc ctgcaacgga tgcaggaccg
     2761 ctcttctgcc aaccggtcca tccgttcgcc tcttttgacc tcattctgta ttccatcatt
     2821 cacctctacg gatacattgg tgactggctt gagaaagcac tctagaaggc tcagtcgtta
     2881 ttgagactct aacttgctca catgactgtt tcatgaacgg atcatgaaga ctttttcatg
     2941 aacatggctt cgatattggc gcgatgcgca gcatacgcgg cagcaccgcg gtacgaagaa
     3001 gcgaaggaag aaatcaggaa actcggaggg ctgctggact aggaaccgtc tcttgaatgg
     3061 ggataggcaa ccgtagggta aagaccgaac cctcattgac acgactggtc accgagatcg
     3121 ttccgccgtg ggctgccgcg attcccttca cgataggaag tcccagcccc gtaccacccg
     3181 agtcttttgc gcgggcccaa tcggtccgat agtaccgctc gaagaggtga ggaaggtgct
     3241 caggttcaat gccatgaccc gtgtcttcga caatgacagc gacccattgg tcgaccgtct
     3301 gcaagcggac ggtcacagtg ccacccgaag gggtatagcg gagagcattg tcgagcaaat
     3361 tcatcactgc ctgtttgatc cactgtgcat caccgtacac atttggcact ggttgggatt
     3421 caaacgataa agtgatccgg cgctcatcaa acaatatgtt caattcagcc aagagatcag
     3481 ggatcaaggc tcccagggcc agtggcgcaa gatgaactgg aggcgtatcg cctgtgaact
     3541 tcgcgagcgt caggagggaa cgggtcagcg agatgagccg tcccacttgt tcgagattgt
     3601 tgatgagggc ctcgcgatac tcttcgtggg tcctggcttt gaggagcgta acctccaagt
     3661 gaccttgcat aacggtaagg ggcgtctgca tttcgtgagc agcgtggtcg acagatcgcc
     3721 gttgactttc cacactccgc tggaaccggt ccatcacgga attgaatgct tgcgtaagcc
     3781 gtcgaaattc ctgatagggc gaaccgagaa tcagccgttt cccgagatcg gcttcggaca
     3841 tcgtttccgc ttcggtgctt agcacagcga gggggctcag aactttctta gccagccaac
     3901 cgctgcctag ccaggcgaca agaaggatgg ttgccgcacc catcgataat agaagcacga
     3961 gctctttgag tgtctctcga tagagtcgca gggaggcctc ggcctgcagc acgtactgca
     4021 cttttccttc cctgaaaatg ggtaagaaca ggtgccgaac aggtgtgcca tcgaccgact
     4081 ctacggtttc aactacgaca tgtccgagac gagcttgttc aaacacctgc tcgctcatcg
     4141 gctgctgggg ctctgcgtcg gacgcgttcc atagcagcac tccttctggc gagaagacgc
     4201 gaagagaatg ggtaactccc gacacttgaa gttgttcttt ctcacttcgg ctcacgtcgg
     4261 cagatacgac acgatcgtcc atcggagcct tcataaggtc gggattgtgc tcaacgatct
     4321 tcgccagcgt ctcggccaat cccagcagcc gcgcatcgac gaagcggcgc atcaaggtgt
     4381 cgccgcctgc atagatcaca atagaaaaga cgaggagaag tcctgctatc aaaatcagcg
     4441 cagattgccg gatcaaacca tgaagagatt tcattcgatg ggtttcggtt cctctagcat
     4501 gtaccccgcc ccacgtacgg tcacgatcag tgggggcgaa aactctcggt caattttcga
     4561 gcgcagggcg cggatatgtg catccacgat attcgtcatg ggatcgtagc taatgtccca
     4621 cacgtgttcg atgatcgccg tgcgcgttaa cacccggttc ttgtttcgta acaggaactc
     4681 cagtaaggca tattctttgt tggtgagtat gatgtctttg ccagagcgag tcaccttgcg
     4741 tgaagccgga tccaggtgga gatctgcggc cgtaagacgc gtcggctgtt gagccccgcc
     4801 tcgcctgagc aaagaccgga cccgggccag caattctgca aaggcaaacg gcttcgtcag
     4861 aaattggtcc gcaccaatat cgaaggc
//
LOCUS       DASSCQ010000012         4653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_630888, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000012 DASSCQ010000000
VERSION     DASSCQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630888"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..926
                     /locus_tag="VFH05_00900"
     CDS             12..926
                     /locus_tag="VFH05_00900"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6676803.1"
                     /translation="MADNRSILVTGAAGRLGAIGRTVAGLLLDRGLPVRAMVRREDER
                     AAALRAAGADVLLGDLLEPADVYRVVNGCRRIYFGMSLSPGYLEATMIMAAVARELEV
                     EALVNMSQMTVSQMSIQQTTASPQQRQHFLSEQALAWSGLPVVTIRPTVFLEGFFLPL
                     TSPSVRDRGRIELPFGQGKTSPVATADVARVIAAVLADPIPHLGRIYELTGPCSQDMH
                     GVAREYSDALNREVVYSDIPPADWERELKQTGLPEHLIRHLLTMADLNRAGRYDRMSD
                     GVERMTGRPAMSIREFVSLHADAFGGRR"
     gene            complement(1041..1418)
                     /locus_tag="VFH05_00905"
     CDS             complement(1041..1418)
                     /locus_tag="VFH05_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6676804.1"
                     /translation="MSRVSVRYIIDDVPAAMRFYTTLLGFTVERDASPAFAAVVRDGV
                     RLLLSGDGSSGKRPLTDGRQQVPGGWNRVHLEVSNIAEEVERLRGSGVSFRMEEIVTG
                     PGGAQIILDDPSGNPVELFQTGH"
     gene            1523..1996
                     /locus_tag="VFH05_00910"
     CDS             1523..1996
                     /locus_tag="VFH05_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676805.1"
                     /translation="MDPFRIVPLIKTMWKLSSSLIGVHRERDIAIFHKLDSILDESRA
                     KSILDYSFFTECLRFEERELLYKFEDALQRVENRYLHPVIQVRATALARQMSELLRTV
                     GATFRSDDGEIYRFHPDPIDPTAYDREWDKLHDQVEKTSKAYRTYRQAVKKRLQV"
     gene            2015..2725
                     /gene="pxpB"
                     /locus_tag="VFH05_00915"
     CDS             2015..2725
                     /gene="pxpB"
                     /locus_tag="VFH05_00915"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HET6676806.1"
                     /translation="MNQPLQATRNGFAVLPLGDAAVTVEFGRTIEPSLNEHALAFAET
                     VRIQRWEGVIDIVPAYASVTIHVDPFRLPVSTLSARLRDLSYDGKRPLSGLTHRIPVL
                     YGDKWGPDLHDVAAFARQSPAETIRLHHATHYRVYMLGFSPGFPYMGVVPASIAMPRL
                     PTPRTIVPPGSVGIAESQTGIYPTATPGGWRIIGRTPVSLYRPQSSSPFLFSPGDHVQ
                     FYPINADEFTRISNAPPT"
     gene            2709..3479
                     /locus_tag="VFH05_00920"
     CDS             2709..3479
                     /locus_tag="VFH05_00920"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HET6676807.1"
                     /translation="MLRRLDVNSDMGERDTAEGLALDAALMPLITSVNIACGGHAGSP
                     DLMRRTASAAQQHGVTIGAHPGLPDRETFGRAERPVRANEIRALVSEQVLACGRVLQT
                     EGFTLRHVKPHGALYTMAARDEEIAEAIVRGVRDVDASLLLYALAGSTLARVGKAAGL
                     KVIQEGFADRAYRADGGLVSRRDIGAIIEDQDRVREQLRLLLNETVMTVERRLIPMSI
                     HSLCLHSDTPQALVLARLIREELQRSGIAVMAASHDDR"
     gene            3466..4425
                     /locus_tag="VFH05_00925"
     CDS             3466..4425
                     /locus_tag="VFH05_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HET6676808.1"
                     /translation="MTTAKSTSIRVVKPGWCTTVQDLGRHGYQHYGVSVSGAMDRLGL
                     VIANRLVNNPDVAAALEITIVGPELVFEDAAVLAITGADLSPAINGQSVPIWTTLAIQ
                     AGSRLTFGRRRTGARSYIAVAGTLDIPSTWGSRSTHISSGTGGFNGRALLADDVLRVN
                     VDQRERPLIGAMLAADSRPLYVDAPTLRVISGPQHANAAALSILTATPYRLTSQSNRM
                     GYRLEGQRLHEDSSPSRLSDGTAMGALQIPPDGSPILLMADRPTTGGYPKIAAVISAD
                     LPQAAQLQPGDTVTFRTTTLAEAESALVNQWRQINDALPARKW"
     gene            complement(4467..>4653)
                     /locus_tag="VFH05_00930"
     CDS             complement(4467..>4653)
                     /locus_tag="VFH05_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HET6676809.1"
                     /translation="GRRSIRGWPSGTARDSEDTLNFCALTGIRPMVETLPLEQAAAGY
                     ERMLSGKARFRVVLTVN"
BASE COUNT          921 a         1421 c         1360 g          951 t
ORIGIN      
        1 aatggtgagc gatggcagat aaccgatcaa tcctcgtgac aggagcagcc ggtcgcctgg
       61 gggcaatcgg ccgaaccgtg gccgggctcc tgcttgaccg cggccttccg gttcgcgcca
      121 tggtccgtcg tgaggacgag cgggcagcgg ccttgcgagc ggccggcgcc gacgtgctgc
      181 tcggagacct cttggaaccg gccgacgtct atcgggtcgt caacggctgt cgtcgaatct
      241 acttcggcat gtcgctgtcg cccggctacc ttgaggccac catgatcatg gcggcggtcg
      301 cacgtgaact cgaggtggaa gccctggtca atatgtccca gatgaccgtc tcccagatga
      361 gtattcaaca gaccaccgcg agccctcagc aacggcaaca ctttctcagt gagcaggcgc
      421 tggcgtggtc cggcctcccg gtcgtcacga ttaggccgac ggtcttcctc gaaggcttct
      481 tcctcccgct caccagtccg tccgtccgcg atcgaggccg catcgaactg ccgttcgggc
      541 aaggcaaaac ctctccggtt gcgacggctg atgtcgcgcg ggtcatcgct gccgtcctcg
      601 ccgacccgat tccacatctg ggccgaatct acgaactcac cggtccgtgc tcgcaggata
      661 tgcatggggt cgcgagggaa tattcggacg cgctcaaccg tgaagtcgtg tactcggaca
      721 ttcccccggc ggattgggaa cgggagttga aacagactgg attgccggag cacctcatcc
      781 ggcatctact gacgatggct gacttaaacc gagcgggtcg gtatgaccgg atgtcggacg
      841 gcgtcgagcg aatgactggc cgaccggcaa tgagcatacg ggaatttgtg tcgcttcacg
      901 cggatgcctt cggcggtcgt cgataatggt tgtcagtcac gccgacgctg gcatcgctcc
      961 aggcggcccg ttcgctccgc cccgtgacat ggtacatgca ggcgaaagtt gagcgaggag
     1021 atggcgcccc gctacgcatc ttaatgtcca gtctggaata actccactgg gttccccgat
     1081 gggtcgtcga gaatgatctg tgcgccaccc ggcccggtca caatctcctc catgcggaag
     1141 gagacccccg acccgcgtag ccgctcaact tcctcagcga tattgctcac ctctagatgc
     1201 actcggttcc agccgcctgg gacctgctgg cggccgtctg tcaatggccg cttccccgag
     1261 ctgccgtccc cgctcagcaa gaggcgcact ccgtcccgta cgactgctgc gaaggcgggg
     1321 gaggcgtccc gctccaccgt gaaaccaagg agcgtcgtat agaaacgcat cgcagctgga
     1381 acatcatcaa tgatgtaacg aacgctgacc ctggacatcg cgacttcctc ctgcaagttg
     1441 cctttagcca acccgcctta cttcatgtac tatgaccctt tcctgcgtat gtagcccaaa
     1501 catggcatcg ccagagacaa cgatggatcc tttcagaatc gttcccctga tcaaaacgat
     1561 gtggaaattg agcagttccc tcattggcgt gcaccgcgag cgagacattg ccatttttca
     1621 caagttggat agcatactcg atgagtcacg cgccaagagc attttggact atagcttctt
     1681 tactgaatgc ctccgttttg aagaaagaga actgctgtat aagttcgaag atgccctcca
     1741 gcgtgtggag aaccggtatc tgcacccggt cattcaggtt cgcgcaacag cacttgctcg
     1801 gcaaatgtct gagttgttgc gcactgtcgg tgcgacgttc cgctcagacg acggagagat
     1861 ctaccgcttt caccctgatc ccatagatcc caccgcctac gatcgggaat gggataaatt
     1921 gcacgaccaa gttgaaaaaa catcgaaagc gtataggacg tatcgccaag cggtcaagaa
     1981 gcgactccag gtgtaattgg acgatagggt tgccatgaat cagcctctcc aggcgacgag
     2041 gaatggcttc gcggtgctgc ccctcggaga tgcggccgtg acggtggagt ttggccgcac
     2101 gatcgagcca agccttaacg agcacgccct cgctttcgca gagacggtgc gtatccagcg
     2161 atgggaaggc gtgattgata ttgtgccggc gtatgcctct gtcaccattc acgtcgatcc
     2221 atttcgactg cctgtttcca ccctctctgc acggctccgt gatctttctt acgatggaaa
     2281 gcggcccctt tccggtctga ctcatcgcat ccctgtattg tatggagata aatggggtcc
     2341 tgatcttcac gatgtcgccg ccttcgccag acaatcacca gccgagacaa tccggttgca
     2401 tcacgcgacc cattacaggg tgtatatgtt gggattcagc ccgggatttc cgtacatggg
     2461 ggtggtcccg gcatctattg ccatgccccg cctgcccacg cctcgtacca ttgtcccccc
     2521 cggttctgta gggattgcag aatcgcaaac gggcatctat ccgaccgcga ctcccggcgg
     2581 gtggcggatc atcggacgca cgcctgtcag tctctatcgg ccacagtcct catcaccctt
     2641 tcttttcagt ccaggcgacc acgtgcagtt ttatcccatc aacgcggacg agttcacccg
     2701 gatttccaat gctccgccga cttgatgtga acagcgacat gggcgagcgc gacaccgccg
     2761 aagggctggc gctcgacgcg gcattgatgc cgctcattac gtcggtcaat atcgcctgcg
     2821 gtgggcatgc cggcagcccg gatcttatgc gcaggacggc ctctgccgcg cagcaacacg
     2881 gtgtaaccat cggagcccat ccgggtttgc cggatcggga aacgtttggg cgcgccgagc
     2941 gccctgtcag ggccaacgaa atccgcgcgc tcgtgagtga gcaggtcctg gcctgtggcc
     3001 gtgtcctgca gacggaaggc ttcacgctac ggcatgtaaa gcctcatggc gccctctata
     3061 ccatggccgc tcgagatgag gagatcgcag aagccattgt caggggcgtg cgggacgttg
     3121 acgcctcgct tttgttgtat gccttggccg gatcgacgtt ggcgcgtgta ggaaaggcgg
     3181 cagggctgaa ggtgatccag gaaggatttg ccgatcgggc ataccgagcc gatggtggat
     3241 tggtatcacg acgcgacata ggagcgatta tcgaagatca agaccgcgtg cgagagcagc
     3301 ttcgcctgtt gctcaatgag accgtcatga cggtcgagcg tcgactgatt ccgatgtcga
     3361 ttcacagtct gtgcctgcat agcgataccc ctcaggccct cgtgctggcg cggctcattc
     3421 gggaagaact tcaacggagt ggaattgcag tgatggcagc cagtcatgac gaccgctaaa
     3481 tcgacctcga tccgagtcgt caagcccgga tggtgcacaa cggtccagga tctcgggcgt
     3541 catggttacc aacactatgg cgtgtcggtc agcggggcga tggaccgact cggcctcgtc
     3601 atcgccaatc gtctggtcaa caacccggat gtggccgctg ccctcgaaat caccatcgtg
     3661 ggccccgagt tggtctttga agacgccgcg gtcctggcga tcacgggcgc agatctgtcg
     3721 ccggccataa acggccaaag cgtcccgatc tggactacgc tggccatcca ggcaggaagc
     3781 cgcctcacat ttggacgacg acgaacgggt gctcgatcct acatcgccgt ggccggaacg
     3841 ctcgatatcc cgtcgacatg gggcagccga tccacgcata tatcaagtgg cacgggcggc
     3901 ttcaatggac gtgcactctt agccgatgac gtgttgcgag tgaatgttga tcagcgcgag
     3961 cggcccctta taggagcgat gctagctgcc gacagtcgcc ccctttatgt cgatgcaccg
     4021 accttacggg tcatctctgg cccgcaacac gcgaacgcgg ctgcgctgtc gattctcacg
     4081 gccacaccgt atcgacttac cagccagtca aatcgcatgg gctatcgctt agaagggcag
     4141 cggcttcacg aggattcctc gccttctcgg ctctcggatg gaacggcgat gggagcactc
     4201 caaattccac cggatggatc tcccattctg ttgatggcgg accggccaac aaccggcggg
     4261 tatcccaaga tcgcggcggt catttctgca gatcttcctc aggccgcaca actccagccc
     4321 ggcgatactg tgacctttcg cacgaccacg ctggcggagg ccgagtcggc attggtgaac
     4381 caatggcgcc agatcaatga cgccctgcca gcccggaagt ggtaaaccca cgctgcctcg
     4441 tgggcgtctc cgcagtggag tatgaattag ttcacggtca gcaccacgcg gaatcgcgct
     4501 ttgccgctta acatgcgctc atagccagcc gcggcttgtt cgagggggag cgtctcaacc
     4561 ataggccgta ttccggtgag cgcacaaaag ttcagcgtgt cttcggaatc gcgggccgtc
     4621 cctgaaggcc atcctcgaat ggatcggcgg ccg
//
LOCUS       DASSCQ010000013         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_516318, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000013 DASSCQ010000000
VERSION     DASSCQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516318"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VFH05_00935"
     CDS             complement(<1..1316)
                     /locus_tag="VFH05_00935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6676810.1"
                     /translation="MAWLNEFYSAPVAVPLSLACLAIVSLLSWRKQGRQVILALTLFL
                     YVRYLVWRGIYTLNTADWSGLCISSLVLLAEAYGLVQLIFFTFQAWKPFERKSPQIKT
                     YRTVDVFVTVVNEPLDILRRTLIGCTSQDYPADKYKVYVLDDGGREDVLALARSLGCE
                     YLRREGRDHAKAGNLNHALGRTSGDLIAVFDTDHVPTSAFLQKTVGFFDEEKVAIVQT
                     PHHFYNPDIFQKNFHLESELKNEQALFFRVLQSGRDAHNSAFFAGSSGLFRRGPLMEV
                     GGFQTQTITEDIHTSMLVHARGYESRYLNQVLSAGLMPETFSGYLKQRSRWAIGCIQM
                     FVKCNPLMTKGLTWAQSVDYFGSVYYFFHGVPRVVCLAAPLSALLLGISPVAATVPER
                     VNLFGTYFLASLVMMRTVSRGTRNAFWADVYETAMCFSLSKAALGT"
     gene            1656..>2240
                     /locus_tag="VFH05_00940"
     CDS             1656..>2240
                     /locus_tag="VFH05_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6676811.1"
                     /translation="MVIGLGGVFLAIWPWRDFLPETTISTDPVVVATVGTRTITLQAL
                     ERSLALPLYTADLKRQQLLQQGLQHNIDEELLKAEATKRDISVDQLLDEASQSESIAR
                     LANLPGPVKRLTPTPPRDRQPVTPTDPELQAKIRQALLVSLRRQTDIRVTLPVLEPPI
                     LPVSADDDPRMGPDNAPVTIIEFSDFQCPFCQRSV"
BASE COUNT          515 a          643 c          611 g          471 t
ORIGIN      
        1 atcgtgccca gtgccgcctt gctcaggctg aaacacatgg cggtttcata gacatcggcc
       61 caaaaggcat tgcgcgtgcc ccgactcacg gtacgcatca tgacgagcga ggcaaggaag
      121 taggtgccga agaggttgac ccgttccgga accgtcgcag caaccgggga aataccaagc
      181 aagagtgcag acagcggagc ggcgagacag acgactctgg gcacgccgtg aaagaaatag
      241 tacacggaac cgaaataatc gacactctga gcccacgtga gccccttcgt cataagggga
      301 ttacacttca caaacatctg aatgcagccg atggcccatc gcgagcgttg cttcaggtat
      361 ccgctgaacg tttcaggcat cagcccggcc gagagaacct gattcagata ccgtgattcg
      421 tatcctctcg catggacgag catgctggtg tgtatgtcct ctgtgatcgt ctgcgtttgg
      481 aatcccccga cttccatgag gggtccacgc cgaaaaagcc ctgaactgcc cgcgaagaat
      541 gcactgttgt gggcgtcacg gccggattgc agcactcgaa agaacagcgc ctgctcgttt
      601 tttaattctg attcgagatg aaaatttttc tgaaagatgt cggggttgta gaaatggtgg
      661 ggagtttgca cgatcgcgac tttctcttcg tcaaagaagc cgacggtttt ctgcaaaaag
      721 gcggacgtcg gcacatgatc ggtgtcaaat accgcgatca aatcgccgga tgtccggccg
      781 agcgcatggt tgagatttcc agccttggcg tgatcgcgcc cctcccgccg caggtactcg
      841 catcccagag accgcgccag ggcgaggacg tcttcccgac cgccatcgtc caacacatac
      901 accttgtact tatcggcggg ataatcctga ctcgtacagc cgatcaaggt ccgccgtaag
      961 atgtcgagcg gctcgttgac cacggtcacg aacacatcca ccgtccgata ggtcttaatc
     1021 tgaggagact tccgctcgaa cggcttccag gcttggaacg tgaagaatat gagctggacc
     1081 agaccatagg cttccgccag cagcacgagg gagctgatac agagtccgga ccagtcagcc
     1141 gtattgaggg tatagatgcc tcgccaaacc aggtatcgca cgtacaaaaa aagcgtcaag
     1201 gccaggatga cctgccgccc ttgtttcctc cagctgagca agctgacgat cgccagacag
     1261 gcgagggaca gtgggacagc cactggggca ctgtaaaact cattcagcca ggccatacta
     1321 ttttcctttg ttaagagtac ctaggacatt tcactgcgct gcggctcttt gtcctctgcg
     1381 gttgccatga ctcagaggat agaatgggaa agccgtttcg cctataccga ggacggaggg
     1441 aacccgctat acagggggag tacttcgttg gaggggcagg aatgcctcag gaagttttac
     1501 cagtctggag aggaggattg agggttcgag gagccaaatt ccaaggaatt cgtggatttt
     1561 tgagtgcctg ctgctatggt ggtactgcgt agtcaaacac catgacatgt ctaggacgcc
     1621 agctgtcgat ctcccattta ttgatgagcc tcgccttggt catcggtctc ggcggggtct
     1681 tccttgcgat ctggccatgg cgagattttc tgccggagac tacgatctcc accgaccccg
     1741 tcgttgtcgc caccgtcggc acacgtacta ttactctaca ggctttggag cgctctttgg
     1801 ctcttcccct ttacacggct gacctgaagc gccagcaact gctccagcag ggccttcagc
     1861 acaacatcga cgaggagttg ctcaaagcgg aagctaccaa aagggatatc agcgtggatc
     1921 agcttctcga tgaggcatcg caatctgaat cgatcgcccg tctcgcaaac ctcccaggtc
     1981 cggttaagcg gctgactccc accccgccac gcgaccggca acctgtcacc ccgacggacc
     2041 ccgagctaca agctaaaatt cggcaagcat tgctggtgtc acttcgccga cagacggata
     2101 ttcgcgtcac cctccccgtc ctagaaccac ccattcttcc ggtcagtgcc gacgacgatc
     2161 cgcgcatggg cccggacaat gcccctgtga ccatcattga attttccgac tttcaatgcc
     2221 cgttttgtca gaggagcgtc
//
LOCUS       DASSCQ010000014        15284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1127559, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000014 DASSCQ010000000
VERSION     DASSCQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127559"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..609)
                     /locus_tag="VFH05_00945"
     CDS             complement(217..609)
                     /locus_tag="VFH05_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676812.1"
                     /translation="MKRHRTIIPVICLLGLLSFSNLGVAGEGPAGKIDPAALKHQVVG
                     QEAALSLESLTASKTFRDIPSISGNYTVKGTRIMPYIGAGFGHGYTSDVDRSLNSGPS
                     TQTDPGLRSMLGQGLTPSEFQMGLRIPF"
     gene            complement(700..879)
                     /locus_tag="VFH05_00950"
     CDS             complement(700..879)
                     /locus_tag="VFH05_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676813.1"
                     /translation="MKNVEMTVEGTLLTIKVDLSKEFGPSSSGKTIIIASTEGNVTIP
                     NREEKVGLNVYRKKS"
     gene            1588..2574
                     /locus_tag="VFH05_00955"
     CDS             1588..2574
                     /locus_tag="VFH05_00955"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET6676814.1"
                     /translation="MDRHVGKTPLIRLRRLSELTRCEILGKAEFMNPGGSVKDRVALS
                     IIEDAEARGLLRPGGTIVEGTGGNTGIGLTVIGHARGYRTLIVIPETQSREKFDLLRT
                     LDAEVLIVPEKPYSHPDNYNHVARRLAEEKGWFWANQFDNTANRLAHYRTTGPEIWEQ
                     TAGQVSAFVSTVGSGGTLAGTTWYLKERNPNIVIGCADPYGSAMWSWFIHGNTETNDG
                     DSFAEGIGQSRVTKNLEGLAVDAAWRIPDQDALTILYQLVREEGLFLGLSSGINLAGA
                     VRLALERGPGQTIATILCDSGAKYQSKLFNPEWQAANGLRTDLSVETLIAKW"
     gene            complement(2613..3992)
                     /locus_tag="VFH05_00960"
     CDS             complement(2613..3992)
                     /locus_tag="VFH05_00960"
                     /inference="COORDINATES: protein motif:HMM:NF004992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET6676815.1"
                     /translation="MARHFDAIIIGTGQAGPPLAARLASAGMIVAIIERHKFGGTCVN
                     TGCIPTKTLVASAYAAHVARRGAEYGFDVTGNVRVDMKRVKARKDEVAGRSNRGVEDW
                     LRGLKNCTVILGHARFQSCRTVMVNDDVLQAEHIFINVGGRASVPAMPGIQDVPFLTN
                     SSMMDVDFLPERLVIVGGSYIGLEFGQMYRRFGSHVTIVEMGPRLIGREDEDVSEAVR
                     QILEAEGIQVRLNAKCISLAKRNNGAAVRMGCDEGPPEVVGTHVLLAVGRIPNTNDLG
                     LDRAGVATDQRGYIVVDEQLQTNVPGIWALGDCNGRGAFTHTSYNDYEIVADNLLNGD
                     RRRVSDRIQAYALYTDPPLGRCGMTDAEIRKSGRAALAAKVPMSRVGRAVEKGETQGF
                     MRVSVDAETKHILGAAILGTGGDEVVHVLLDVMYAKAPYTVVQRAMHIHPTVAEFLPV
                     VFSNLEPVA"
     gene            complement(4032..4727)
                     /locus_tag="VFH05_00965"
     CDS             complement(4032..4727)
                     /locus_tag="VFH05_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6676816.1"
                     /translation="MSTTQVRSENVTGKAVAAKVDMKLEIVVIPVSDVDRAKEFYERL
                     GWRLDADFDNGKDFRVIQFTPPGSGCSVIFGKSVTAAAPGSAQGLYLIVSDIEAAREE
                     LLGRGIEISEVFHDAGGVYAGTDEPYLFGKLRARGPDPEHRSYRSFASFHDPDGNGWL
                     FQEITTRLPGRIDSTMTTFASANDLASAFRRAEAAHGEHEKNVLGGQRDENWPEWYAA
                     YMVAEQAGTELPM"
     gene            5056..6315
                     /locus_tag="VFH05_00970"
     CDS             5056..6315
                     /locus_tag="VFH05_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6676817.1"
                     /translation="MLTHTATTTHLLQMLSHARAGTDTLFGLVRPEAFYERPVPERHR
                     IIFYFGHLEAFDWNLISQPAAVPSFSPDFDQLFAFGIDPKLGHTQQDERSDWPEIAEV
                     REYNRRLRQTLDDVLHQTSEQLLSIALEHRLMHAETFAYLLHSLSINQKHVPFDQKHV
                     PLVASFPPSAAPIHAMVEISGGTVTLGQRRTEPSGRNPFGWDNEFESHEVAVASFAMS
                     KYKVTNGEYLKFVRAGGKPPHFWRWHEGQWYWRGMWEEVPLPLDWPVYVTHEEASAFA
                     AWSGKRLPTEAEFHRAAYGTKHGEEERAYPWGDEQPDGRRGNFGAYRWDPISVTASPL
                     GDSAFGVSQLVGNGWEWTSTAFEPFPGFQTFSCYPGYSARFFGGDHYVLKGASPRTDA
                     RLLRRSFRNWFRPNFPYVYAGFRCVEA"
     gene            6317..7288
                     /gene="egtD"
                     /locus_tag="VFH05_00975"
     CDS             6317..7288
                     /gene="egtD"
                     /locus_tag="VFH05_00975"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET6676818.1"
                     /translation="MMPSQTVHTDKLREFAVAVSDSLGKVGQRELPSMYLYDELGTAL
                     FEAITLLPEYGLTRAELRLLSRHAGAIVEHLPPPVSVIELGSGSGRKTRWILEALADR
                     EPIAYFPIDVSAAALLKCHQELGHLGSVSMVGLERSYLEGIQEAAARRQGDQTLLVLF
                     LGSSIGNFDPPAAEHFLQDLHRHLMPGDALLLGTDLVKSEADMLLAYDDPTGVTAAFN
                     LNLLARINRELEGDFVLRHFEHEVRYQEVPRRIEMHLRSTRNQTISIRAADFTLTLNE
                     GETIWTEACHKFRPEEIPGLARRTGFLCEAQWIDAEWAFAENLLIAN"
     gene            complement(7285..8181)
                     /locus_tag="VFH05_00980"
     CDS             complement(7285..8181)
                     /locus_tag="VFH05_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6676819.1"
                     /translation="MGRTEIVACLIVGLCVSAGIAGCTREHPITVGSKNFTEQVVLGE
                     IVAQHLEHRLARPVARKFNLGGTMLAHHALVRGDIDLYPEYTGTALTVILKLPPVHDQ
                     AAGIALIRREYQARFGLQWMDPLGVNNTFAMVIREADARKHNVVTLSDAARYAPGWTL
                     GVGYEFQQRPDGLAGLLKTYQLPLHGTPKTMDLGLLYKALEQGQVSMVAGNATDGQLS
                     VLNAVVLQDDQHYFPPYDCGFVVRPEIVKDYPALQQALDELVGRLSNQTMRKLNYQVD
                     GEHRPVRDVAEQFLLEAGLLRR"
     gene            complement(8151..8786)
                     /locus_tag="VFH05_00985"
     CDS             complement(8151..8786)
                     /locus_tag="VFH05_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6676820.1"
                     /translation="MEGLDLISSMLARDIVDLTLEHLYLVGVSMGIAASLAIPVGILL
                     TRRMFLQRWVLGFANVMQTVPSLALFGFLIPLPFLGGIGKHTAIVALVLYALLPILRN
                     TLTGILAVDPTVRESAVAMGMTGRQLLWEVELPLATRTILAGVRIATVTTIGTATIAA
                     AIGGGGLGVFIFRGLASVDTTLLLAGAVPAALIAVAADRGLEWVEQKLSPV"
     gene            complement(8768..9517)
                     /locus_tag="VFH05_00990"
     CDS             complement(8768..9517)
                     /locus_tag="VFH05_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6676821.1"
                     /translation="MSPLVSFRQVATRIGQKDILCNLSFDVEAGETLVLLGRSGSGKT
                     TALKMVNALLLPSAGEVLIEDRPTTRWDPIGLKRRTGYVIQEGGLFPHFTVAANVGLV
                     PRLEGWPSPKIEARVESLLNQVGLPPAQFAQRYPRELSGGQRQRVGIARALAADPPLM
                     LLDEPFGALDPVTRVELQHMLIDLRDKLGKTALLVTHDVREALLLGTRIGVLHNGRLA
                     FFGGRSDFLKAQDEEVRAFHTCLGDTWKDSI"
     gene            complement(9514..11160)
                     /locus_tag="VFH05_00995"
     CDS             complement(9514..11160)
                     /locus_tag="VFH05_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6676822.1"
                     /translation="MADVDLHKVAFPTLDDAQVATLARFGTRRLLRDGEHLFKAGDRE
                     YKFFVVERGAVEIVEHSSGETKRVTLHERHAFGGDVSLVTGRPALISGISRGDTEIFE
                     IARDDIRRIMGERPALGDVLLRAFIARRELLVASDFQGLRVLGASSRDTFRIRDFLAK
                     NQVPFTWIDLNHDPQVGALLQSFGLSEADTPVVVFGSEPLLKNPSTRELAELIGVRRP
                     LTQRVYDLVIVGGGPAGLAAAVYGSSEGLATTVIDAVAPGGQAGTSSKIENYLGFPTG
                     ISGAELATRAILQAQKFGAQFSSPSPVARLTFEKDHAVLWLDDGQQVSTRCVLIATGA
                     EYRKLDVPMREQFEGLGVYYAATPTELQLCRGSEVMVVGGGNSAGQAVMFLSEQTPRV
                     FLLLRGGDLRKSMSSYLADRIEGADNVEVLSNAEMTCMLGDQRLEAVEIKDTRSGETR
                     TIRTSAVFTFIGAIPCTNWLPPEIETDSKGFVKTGRLVSSSPYWTAEREPFFLETSRP
                     GIFAAGDVRLGSTKRVASAVGEGAMAVQLAHEYLGLQLTP"
     gene            complement(11197..11481)
                     /locus_tag="VFH05_01000"
     CDS             complement(11197..11481)
                     /locus_tag="VFH05_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET6676823.1"
                     /translation="MSCSHVTTVEAVTARTAGCEECLKSGSSWVHLRLCLTCGHVGCC
                     DSSPNRHATKHFERTTHPVMASFERGERWAWCYVDQMQLDVPGRVAKSLR"
     gene            complement(11636..11941)
                     /locus_tag="VFH05_01005"
     CDS             complement(11636..11941)
                     /locus_tag="VFH05_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676824.1"
                     /translation="MKMLTIVCREQFEEEVLALFSNLKISGYTVVHGVGGSGETGEVS
                     VTHTSYDHNKLILVALDDDRIDLLVKGVKQLLARLVEERSGDPVPLKAFVQPCEPVV"
     gene            12198..12575
                     /locus_tag="VFH05_01010"
     CDS             12198..12575
                     /locus_tag="VFH05_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6676825.1"
                     /translation="MVKILVIDDDPKDRGLIAAVLEERGYEVIVAENGRRGLMFCHRQ
                     QPDAVVLDLCMPEIDGRSVLQQLCTLYPSLPVVVFSGCRTEEAEEAVLNSGATAFLQK
                     AFSLEHLGLALQEVLPPPNKSDN"
     gene            complement(12624..13208)
                     /locus_tag="VFH05_01015"
     CDS             complement(12624..13208)
                     /locus_tag="VFH05_01015"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6676826.1"
                     /translation="MRTATRRGVIVLSLSLLCSAGLMGETARPEGQAGIPPEVVAEYV
                     YAIVQADRTLYTTHVVERMQELGVTAATEQWKKQGTLPLPAQMLLMTGEAIQGQGTSL
                     RIRLTSLWPINKANGPADEFERLGLERVAKEPQKPYAGIVSQGDRRMFKAIYADRAIT
                     KACVECHNGHSSSSRRDLKLNDVMGAIVISFPSR"
     gene            complement(13219..13782)
                     /locus_tag="VFH05_01020"
     CDS             complement(13219..13782)
                     /locus_tag="VFH05_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676827.1"
                     /translation="MKPTDTLFSALRFARVMAVIAGAIILVPPDRTLAHDGASGHHQG
                     AHASAKPATADVEIVVQEGGHPILKGPQTHGQVLTLVAGEPIVLALRNEDHGPREFIS
                     PLFTKTEIQFLGRATGIFRKDAAGFRVNPGDTLTLQFTAPFSGFRRMYDLIWCGHDGK
                     PGTEIQELLIIMTEEKQPHAASETPGR"
     gene            complement(13801..14283)
                     /locus_tag="VFH05_01025"
     CDS             complement(13801..14283)
                     /locus_tag="VFH05_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676828.1"
                     /translation="MHVCLVPLMVLAMILTGGSRGAFAQGDEREYEPDVILVIKEKAF
                     HMVKGNASGQHSEHPAFSLSSGEEITLELRNEDTLPHEFVSPLFAKVEFQFWGRATMV
                     YTYTANGLRIDPGDTVGLRFDLPKDFSGDMFKFWCNVHGKLHGDVLQGEIFVVKPKHG
                     "
     gene            complement(14285..14809)
                     /locus_tag="VFH05_01030"
     CDS             complement(14285..14809)
                     /locus_tag="VFH05_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676829.1"
                     /translation="MRHIGGISASVIALLAVAAPAAAQSSINEATDAGRPQIIRSENM
                     EPNAEVVMTGQSFQIAKGETAKGFMLLAGTRADIVLRNEDTVAHEFVSPMLFNVPFRL
                     DGNGVFVKLPNAAGVRVDPGRVVRLSFDVPQDTKEFQNLYEVFWCNVHGKQYGNKMRG
                     EVLVVDHRGEIGGG"
BASE COUNT         3237 a         4650 c         4286 g         3111 t
ORIGIN      
        1 tcctgtcgca tccgctgatt gccagcggca agaccagtac gaggatgaac caccaccgat
       61 gccttagacg cataagacgc actctttcct tctttcctgc tgttcataac aaatacgtag
      121 actcggcgca atgtctccgg aacgctttcc catgcctctg atggagcagc aggattggga
      181 gaacgcagca gaacgccgtg gaaggtttga ggctgatcag aacgggatgc ggagacccat
      241 ctggaattca cttggtgtga gaccctgccc caacatgctt cgaagaccgg gatcagtttg
      301 agtggaaggg ccgctattca gtgaccggtc tacatctgac gtatagccgt gaccaaaacc
      361 ggcaccgata tagggcatga tcctggtccc cttcaccgtg tagtttccgc tgatggaggg
      421 aatgtcccgg aacgttttcg aggccgtcaa tgattcaaga gataaggcag cttcctggcc
      481 aaccacctgg tgcttcaaag cggccggatc gattttgccg gcaggcccct cacctgccac
      541 tcccagattg gaaaatgaca agaggcccag caaacatatc accggtatga tcgtacgatg
      601 gcgtttcatc ttctgcgctt gatcggcgag agtccctttc cgtgatgttc aagatataaa
      661 ccacaatacc taagtgtcaa gagccgcgac gcctacccat cacgacttct ttcgatagac
      721 gttgagcccg accttttcct cccgattcgg aatcgtcaca tttccctccg tcgaggcgat
      781 gatgatggtc tttccagacg aagaaggtcc gaactctttg gagagatcca ccttgatcgt
      841 cagcagggtt ccctcgacgg tcatctcaac gttcttcatg gtgctatcct ccaccgttac
      901 gacatcggcc cgctcattat ataacgagtc ggtgactctc tataacatct gcatcgagca
      961 agtgccctct cccgatccgt tctgagcgcg tcgccgacca gcaccgcgtc gaaataggcg
     1021 ccggtttccg acggctcctg ccgcaaatcc gccagccacc gatcgcttca ggctgtcaca
     1081 tgaatcgttg atggggggca tccgggtagt cccatctcgt ccagtgagct gaaccagtcc
     1141 accacctttg cccgcaactt tctcgtcgag attggcgaac gtcaggactg cgaccaaggc
     1201 agcttctcag agatcaggga gcacaactcg gtattcgttc gtgagagtga agatgttgta
     1261 atcgcgctcg tacttttccg agaaaccagg ttcggcgtaa gcggggaccg cgaagaggaa
     1321 cccgaggaga gggatcaccc acatggcaca cacaccctta ttgagtctga gcagtctgat
     1381 atgagcagtc tattcctcgg ctaaagcgcg atgcaaggcg gtaggtttga ggaaacgtgc
     1441 acgtattcgc gcgcgtcggt ggaccaagct cagtttgact cagggattct ttgcagatta
     1501 ctttagggag tgtcctcaac acaatgtgtt gtctcgccaa acgcaaaacc ggcaatctaa
     1561 ttgatgaacg cactacgata tcatggggtg gatcgccacg tcggcaagac cccgctcatc
     1621 cgcctgcgtc gcctttcaga gctgacccgc tgcgaaattc tgggcaaggc cgaattcatg
     1681 aaccccggcg ggtcggtgaa ggatcgcgtc gcgctgagta ttatcgagga tgccgaagca
     1741 agaggattgc tgagacccgg cggcaccatc gtcgagggga caggtggaaa taccggcatc
     1801 ggcctaacgg tcatcggcca cgcccgcggg taccgtactc tgatcgtcat tcccgaaact
     1861 cagtcgcgag aaaagttcga ccttctgcgc acgctggacg cggaggttct catcgttccg
     1921 gagaagccgt actcccatcc agacaactac aatcacgttg cccggcgcct ggcggaggaa
     1981 aagggctggt tctgggcgaa ccaattcgat aacaccgcca accgtctcgc gcactaccgg
     2041 accaccgggc cggaaatctg ggagcaaacc gccggacagg tcagtgcatt tgtttccact
     2101 gtcggttccg gcggcacgct ggccggaacc acgtggtatc tcaaggagcg caatccaaac
     2161 atcgtcatcg gctgcgccga cccctacggc tcggcgatgt ggtcgtggtt catccatggc
     2221 aacacggaaa cgaacgacgg tgattccttt gctgaaggca tcggccaatc ccgggtgacc
     2281 aaaaaccttg agggtctggc cgtggacgcc gcctggcgca ttcccgacca ggacgcactg
     2341 acgatcctct accagcttgt acgcgaggaa gggctgtttc tcggcctgtc ttccggcatc
     2401 aacctcgccg gggcggtgcg gttggccctc gagcgtgggc ctggccagac catcgcgacg
     2461 atcctttgcg actccggcgc gaagtatcaa tcaaagctct tcaatccgga atggcaggcg
     2521 gccaacggac tgaggacgga tctgtccgtc gagactctca tcgccaagtg gtgatcccgt
     2581 aatcgctatc ggctcatcac attggccggc tcctatgcga cgggttccag gttggagaag
     2641 acgacgggga gaaattcggc aaccgtcgga tggatatgca tggcgcgctg taccaccgta
     2701 tacggcgctt tcgcatacat gacgtccagc aacacgtgaa ccacttcatc gcctccggtg
     2761 ccaagaattg ccgcacccag aatgtgtttg gtttccgcgt cgaccgagac tctcatgaac
     2821 ccttgagttt ctcccttctc gacagcccgg cccactcggc tcatcggaac tttcgccgcg
     2881 agtgcagcgc gcccggactt ccgtatttcg gcgtccgtca tgccacaacg gcccaacggt
     2941 ggatcggtgt acagtgcgta cgcctggatc cggtctgaca cgcggcggcg gtctccgttc
     3001 agcagattat ccgccacgat ctcataatcg ttgtacgacg tatgcgtaaa ggcgccgcgg
     3061 ccgttgcagt cacccagggc ccagatcccg ggcacgttcg tttgcaactg ttcatcaacg
     3121 acaatgtacc cacgttggtc ggtcgccacc ccggcccgat cgagacccag gtcgttggtg
     3181 ttgggaatac gtccgaccgc gagaagaaca tgcgtgccta ccacttccgg cggcccttcg
     3241 tcgcatccca tgcggacggc ggcaccgttg ttgcgcttcg ccagggatat gcatttcgca
     3301 ttcaatcgaa cctgtatccc ctcagcttcc aaaatttggc gcacagcttc ggaaacatct
     3361 tcgtcttctc gcccaatcag gcgcggtccc atttcgacaa tggtcacgtg gctcccgaag
     3421 cggcgataca tctgaccgaa ttccaacccg atataacttc cgccgacgat gacgagacgc
     3481 tccggcaaga agtcgacatc catcattgac gagtttgtga gaaacgggac gtcctgaatc
     3541 cccggcatcg cgggcacaga ggcgcgtccg cccacattga tgaaaatgtg ttcggcttgc
     3601 agaacgtcat cgttgaccat gacagtgcgg caggactgaa agcgggcatg gcccaggata
     3661 accgtacaat tcttgagccc tcgcaaccag tcctccacgc ctctgttcga ccgtccagca
     3721 acttcatctt tgcgtgcttt cacacgtttc atatcgacgc gcacattgcc agtgacgtcg
     3781 aaaccatatt cggcgccccg acgggccaca tgggccgcgt aggcgctggc gacgagcgtc
     3841 ttggtcggga tgcatccggt attcacacag gtgccgccaa atttgtggcg ttcaataatg
     3901 gcgacgatca tgcccgcgga agccaaacgc gcagccaacg gcgggcctgc ctggccggtc
     3961 ccgatgatga tggcatcgaa atgccgcgcc acgagacacc tccttgaagg aagagtgccg
     4021 gcctgagtcg cttacatcgg cagctccgtc ccagcctgtt ccgccaccat gtatgcggca
     4081 taccattcgg gccagttttc gtcgcgctgt ccgcccagca cgtttttttc gtgctctccg
     4141 tgggcagctt ctgcacgccg aaacgcgctc gccagatcgt tagccgatgc aaaggttgtc
     4201 atcgtggagt cgatgcgacc gggcagccga gttgtaattt cctggaacaa ccagccgttg
     4261 ccgtcgggat catggaacga ggcgaacgac cggtagctgc gatgctcggg atccggaccc
     4321 ctggctcgga gtttcccaaa caggtacggc tcgtccgtgc cagcgtagac acccccggcg
     4381 tcatggaata cctcgctgat ctcgatgccg cgacccagca gctcttcgcg ggcggcctcg
     4441 atgtcagaaa cgatcaagta caggccctgg gcggagccgg gtgctgccgc ggtgacactc
     4501 ttgccgaaga tgaccgaaca cccggagcct ggcggtgtaa actggatcac acggaaatct
     4561 ttaccgttgt cgaagtcagc gtcgagccgc cacccgagcc tctcgtagaa ctcctttgca
     4621 cggtcgacat cggagactgg gatcacgacg atctcgagct tcatatccac tttcgcagcg
     4681 accgccttgc cggtcacgtt ctcactacgg acttgagtgg tgctcatggc gacctcctag
     4741 gttcgggcag gatgccgttc gccgaaacaa ttcgcattcg catttaccgg cggactgggc
     4801 ctcacaatgg aaaaggttgt cggtgacacg atccaaggat ctcaggaaag taatcccaac
     4861 gaatcggaaa tgctgtcgtg tcgagctaca ctcaggccga gaaagtctgg acttttttat
     4921 cacatcgaca actaggtgaa gccctgagcc ctaggaagtt tccccatgta catgacctcg
     4981 cgctcgcctg tacggtgacg gaggacctgg cgcggtgtta tcgtcaggcg gttcgttgga
     5041 aaggtgatgg aaccgatgct cacacataca gccacgacaa cccatctcct acaaatgctg
     5101 agtcacgctc gtgcaggtac cgatacccta ttcggcctcg tgcgacccga ggccttttat
     5161 gaacgaccgg ttcccgagcg ccaccggatc attttttact tcggtcatct cgaggccttc
     5221 gactggaacc tcatcagcca accagcagcg gttccatcat tctcgccgga cttcgaccaa
     5281 ctctttgcct tcgggattga tccgaaacta ggacacaccc aacaggacga gcgctcggat
     5341 tggccggaga tcgcagaggt ccgagaatac aaccgtcgcc ttcggcagac actggacgac
     5401 gtgttgcacc agacatcgga acaactgctc tccatcgcac tggaacatcg gttgatgcac
     5461 gccgagacct ttgcatatct cctccattcc ctatcaatca accaaaagca tgtcccgttc
     5521 gaccaaaagc atgttccatt agtggcgtca ttcccaccga gcgcagctcc cattcacgcg
     5581 atggttgaaa tttccggcgg cacggtcacc ttaggtcaac ggcgcacgga gccctccggc
     5641 aggaatccgt ttggatggga caacgaattt gagagccatg aagtggctgt cgcctcgttt
     5701 gccatgagca agtacaaagt gacgaatgga gagtatctca aattcgttcg cgccggcggc
     5761 aaaccacctc acttctggag gtggcatgag ggccagtggt actggcgagg catgtgggaa
     5821 gaggttccgc tgccattgga ttggccggtc tatgtcaccc acgaagaggc atccgccttt
     5881 gccgcctgga gcggcaagag attacccacc gaagccgagt ttcatcgagc ggcgtacggg
     5941 acgaagcatg gtgaggaaga acgggcctat ccgtggggcg atgagcagcc tgatggccgg
     6001 cgagggaact tcggcgcgta ccgatgggat cctatttccg tgactgcctc cccgctcgga
     6061 gacagcgcct tcggagtttc tcaactggtc ggcaacggat gggagtggac gtccacggcc
     6121 ttcgagccat ttccaggatt tcaaaccttt tcatgctatc ccggctactc ggcccggttc
     6181 tttggtggcg accattatgt gctcaagggg gcatccccac ggacagacgc tcgattgctg
     6241 cggcgatcat ttcgcaactg gttccggccc aactttcctt atgtctacgc cggatttcgg
     6301 tgcgtggagg cttaatatga tgccctcaca gactgtgcac accgacaagc tccgcgagtt
     6361 cgccgtcgca gtgagcgaca gccttgggaa ggtcggccaa cgtgaactgc cgtccatgta
     6421 cttgtacgat gagctgggca cagcattatt tgaagcgatc accctgctgc ccgaatatgg
     6481 gctgacgcgt gccgagctgc ggctcctgag tcgccatgcc ggagccatcg tcgagcatct
     6541 ccccccgcct gtctcagtga tcgagctggg aagcggcagc ggtcgaaaga cccgctggat
     6601 attggaagca cttgcggacc gtgagcccat cgcctatttt cccatcgacg tgtcggcggc
     6661 ggcactcctc aagtgccatc aagagctggg acatctcggc tcggtcagca tggtgggatt
     6721 ggaacgatcg tatctcgaag ggattcagga agccgcggcc cgccgccagg gggatcagac
     6781 gctcctggtc ttgttcctcg gaagcagtat cgggaatttt gacccgccgg cggcagagca
     6841 ttttctacag gaccttcacc ggcatttgat gcctggcgac gccctcctcc tggggactga
     6901 cctcgtgaaa tcggaggcag acatgctgtt ggcctatgat gacccgacgg gagtcactgc
     6961 tgccttcaat ctgaatctgc tcgctcggat caatcgggaa ctagagggag attttgtcct
     7021 acggcacttc gaacatgagg tccggtatca agaggtgccc cgtcggatcg aaatgcatct
     7081 ccgctcgacc aggaaccaaa ccatctcgat tcgcgccgcg gacttcaccc tgacgttgaa
     7141 tgaaggcgaa acgatctgga cggaagcctg ccataagttc cgccccgaag agattcctgg
     7201 tctcgctcgt cggacgggat tcctgtgcga agcgcaatgg atcgatgcgg aatgggcctt
     7261 tgccgaaaat ctcttgatcg ccaattaacg acgaagcaac cctgcttcca gtaagaactg
     7321 ctccgccacg tcccgtacgg ggcgatgttc gccgtccacc tgatagttta atttgcgcat
     7381 ggtctgatta ctgagccgtc cgaccagttc gtcgagtgcc tgctgcaagg cgggatagtc
     7441 tttcactatc tcagggcgca cgacaaagcc gcagtcgtac ggagggaagt aatgctggtc
     7501 atcctgcagg accaccgcgt tcagcaccga caactggccg tcggtggcat tccccgccac
     7561 catgcttact tgtccctgtt cgagcgcctt gtagagtagc ccgagatcca tggtcttcgg
     7621 cgtgccgtgg agaggaagct gataggtctt caagagtccg gcgagcccat ccggccgctg
     7681 ctggaattcg tatcccacac caagggtcca accaggagca taccgcgccg catcgctcaa
     7741 cgtgacaacg ttgtgtttcc gagcatctgc ttcgcggatc accatggcaa acgtattatt
     7801 gacgcccaac ggatccatcc attgcagacc aaatcgcgcc tgatactcgc gccggatgag
     7861 cgcgatgccg gcggcctgat cgtgaaccgg cggtaatttg aggatgacgg tcagcgcggt
     7921 gcccgtgtac tcaggataaa ggtcaatgtc accgcgcacg agcgcgtgat gcgccaacat
     7981 ggtgccaccc aggttgaatt tacgagcaac cggacgggca agacgatgtt ccagatgctg
     8041 cgcgacgatc tcgccgagga cgacttgctc ggtgaaattc ttcgagccga ccgtgatcgg
     8101 gtgttctctg gtgcagccgg cgatgccggc tgagacacag aggccaacga tcagacaggc
     8161 gacaatttct gttcgaccca ttccagccct cgatcggctg cgaccgcaat caaggcggcc
     8221 ggtaccgcac ccgctagcag cagcgtcgta tcgacggacg ccagaccacg aaaaataaac
     8281 acgccgagtc cacccccgcc aatcgccgcg gcaatcgtcg ccgtcccgat cgtcgtgacc
     8341 gtcgcgatgc gcacgccggc cagaatcgtc ctggttgcca gcgggagctc gacttcccac
     8401 agcagctgcc gtcccgtcat gcccatggcg acggccgact ctcgcaccgt cggatccacg
     8461 gccagaatgc cggtgagcgt attgcgcagg attggcaaga gcgcatacag cacgagcgcg
     8521 acgatggctg tgtgcttccc gatcccgccg agaaacggga gcggaattag aaatccgaac
     8581 agcgccagac tcggcacagt ttgcatgacg ttcgcgaagc ccaataccca tcgttgaagg
     8641 aacatgcgcc gggtgaggag aatgccgacg ggaatggcca gcgacgcagc gattcccatg
     8701 gacacgccaa ccagatacag atgttcgagc gtcagatcga cgatgtcgcg cgccagcatg
     8761 gaagatatta gatcgagtcc ttccacgtgt cccccagaca ggtatggaac gcccgcactt
     8821 cctcatcttg ggccttgaga aagtcggagc gtccaccgaa aaacgccaat cgaccattat
     8881 gtaggactcc gatgcgggtg ccgagcagga gcgcttcccg gacatcatgg gtaacgagca
     8941 gcgccgtctt cccaagtttg tcgcgcaagt cgatgagcat gtgctgcaat tccacacgcg
     9001 tcaccggatc aagcgcgcca aacggttcat ccaacagcat caggggcgga tccgccgcga
     9061 gggcgcgggc gatgcccact cgctgacgtt gaccgccaga caactcgcgg ggataccgtt
     9121 gggcgaactg tgccggagga agcccaacct gattcagcaa cgactcgacg cgagcctcga
     9181 tcttgggcga tggccagcct tcgaggcgag ggaccaatcc tacattggca gccaccgtga
     9241 agtgcggaaa gaggccgcct tcctgaatga catacccggt tcgacgtttg agaccgattg
     9301 gatcccagcg ggtcgtcggt ctgtcctcaa tcaagacctc gccggcactg ggcaagagca
     9361 gggcattcac cattttcaag gcagtggtct tgcccgagcc gctacgaccc aggagcacta
     9421 gcgtttcgcc ggcctccaca tcgaaactga ggttgcagag aatatctttt tgcccgatac
     9481 gagtcgcaac ctgccggaag gagacaagag ggctcatggg gtcagctgga ggcccaggta
     9541 ttcgtgagcc agctgcaccg ccattgcacc ctcccccacc gcagaggcga cacgcttggt
     9601 ggagccgagc cggacatccc cggcggcgaa gatgccggga cggctggttt ccaggaagaa
     9661 cggctccctc tcggccgtcc agtacgggga ggatgacacc agtcgccccg tcttcacgaa
     9721 acctttcgag tcggtctcta tttcaggggg caaccaattc gtacagggga tcgcgccgat
     9781 aaacgtaaac acggcagagg tccggatcgt gcgcgtctcg ccgctgcgag tgtccttgat
     9841 ttcgacggcc tccagccgtt ggtcccccag catgcaggtc atctctgcgt tggagaggac
     9901 ctcgacgttg tcggcgccct cgatgcggtc cgccagatag ctggacatgc tcttacgcag
     9961 gtcgccgccg cggagcagca ggaacacgcg cggcgtctgt tccgaaagaa acatgacggc
    10021 ttgtccagcc gaattgccgc cgccgaccac catgacctcc gagcctcgac agagctgcag
    10081 ctcggtcggc gtcgccgcat agtagacgcc caagccctcg aattgctcgc gcatgggcac
    10141 gtccagcttg cgatactcgg ccccggtcgc aatcaacaca catcgagtcg agacctgctg
    10201 gccatcgtcg agccagagga cggcatggtc cttctcgaac gtcaagcgcg caaccggcga
    10261 gggagaagag aactgggcgc cgaatttctg tgcctgcaaa atggcgcggg tcgcaagctc
    10321 ggcgccggag atgccggtgg ggaagccgag gtagttctcg atcttggagg acgtcccggc
    10381 ctgtcctccg ggggcgaccg catcgatcac cgtggtagcc agtccttccg atgatccgta
    10441 cacggccgcc gcgagccccg ccggaccgcc accgacgatg accaagtcat agacccgttg
    10501 cgtgagcggg cgtctgactc caatcagttc ggccaattcc cgagtcgagg gattcttcag
    10561 caacggttcg gatccgaaga cgacgacggg ggtgtcagcc tcgctgaggc caaagctctg
    10621 gagcagtgcg ccgacctgcg ggtcgtgatt cagatcgatc caggtgaatg gcacctgatt
    10681 cttggcgagg aaatctcgaa tgcggaaggt gtcgcgcgag ctggcgccga gcacgcggag
    10741 tccttgaaag tccgacgcca cgagcaactc gcgccgggca ataaaggcgc gcagcagcac
    10801 gtcgcccagg gccggccgtt cgcccatgat ccgcctgatg tcatcgcgtg caatttcgaa
    10861 gatctcggtg tcgccacggg aaatcccgct gatcagcgcc ggccggccgg tcacgaggct
    10921 gacgtcgccg ccaaacgcat gccgttcatg caacgtcacc cgcttggtct cgccgctcga
    10981 atgctcgaca atctccaccg cgccgcgctc gacgacgaag aatttgtatt cacgatcgcc
    11041 cgccttgaaa agatgctcgc catcgcgcaa aagacgtcgt gtgccgaacc tggcaagcgt
    11101 ggcgacttga gcgtcgtcga gcgtcggaaa cgcgaccttg tgcaagtcca catcggccat
    11161 ggtgtctccc ctctcgccgc ccaagagttt ccgtggctac cgcagggact ttgcgaccct
    11221 tcctggcacg tcgagctgca tctgatcgac gtaacaccag gcccaccgct cgccgcgctc
    11281 aaacgaggcc attaccggat gagtagtgcg ttcgaaatgt ttggtggcat ggcgattggg
    11341 cgagctgtcg caacagccga cgtggccgca ggtcaggcag agacgcagat gcacccacga
    11401 cgatccactc ttcaggcatt cctcacatcc cgccgtgcgg gccgtcacgg cctcaacagt
    11461 cgtcacgtgt gaacaagaca tgagtcacct cacaagcatc aagagcgccg agaagctcgc
    11521 gttcattgat cgtaatcctc ggatcaatga gaagcaatgg ttggctgtca atggtgaaga
    11581 gacggcgtca cactctgacg cctaagctga caaccaatag cgcatgcgtt gctcgttaca
    11641 cgactggttc gcaaggctga acgaaagcct tcagcggaac cggatccccg gaacgttctt
    11701 cgaccagccg ggccagcaac tgcttgacgc ctttgacgag gaggtcgatc cgatcatcgt
    11761 cgagcgccac aaggatcagc ttgttgtgat catacgatgt gtgcgtgacc gagacctcgc
    11821 cggtttcgcc gctgccgccc accccatgca ccaccgtgta ccctgaaatc ttcaaattgc
    11881 tgaaaagagc gagcacctct tcttcgaact gctctcggca gacaatggtc agcatcttca
    11941 cgggcattcc tcctgatcat tggagcctta ttgtgcctgc cgcgtacgga tgaagtcacg
    12001 ccgtatcgtc cgcaccattc cgccgttagg ccttgaagcc actgccttcg ccaggtcgtg
    12061 tcccgaccct cccgccaggc gggtgctcag aacatgggga gcgctcgctc aactccaatt
    12121 ttctcaatga aaaagactcg aaggctgcta tcctgtcaag gacgtatggt ctgacaaacg
    12181 aggagatgag agctcatatg gtgaagattc tcgtcataga tgacgatccg aaagaccgtg
    12241 gcctgatcgc agccgtcttg gaggaaagag ggtatgaagt cattgtggca gagaatggtc
    12301 gaagagggtt gatgttttgt caccgccaac agcctgatgc tgtcgtgtta gacctctgca
    12361 tgcctgaaat agacggccgc agcgtcttgc agcagttgtg tacactgtac ccctccctac
    12421 cagtcgtagt atttagtgga tgtcgcacag aagaagctga ggaagcggtg ctgaacagcg
    12481 gcgccacggc ctttctccaa aaggcatttt cgctggaaca cttaggattg gcactacaag
    12541 aagtcctgcc gcctcccaat aaatctgaca actgaacatt ccaacgcaag ctgtctcagc
    12601 gttgtgatca agacaacgtc tgctcaccgg gacggaaatg aaatgacgat cgctcccatg
    12661 acgtcgttga gcttcaggtc tcgcctgctg ctcgacgaat ggccattgtg acactcgacg
    12721 caggctttcg tgatcgcccg atcggcatag atggccttga acatgcgccg gtctccctgg
    12781 ctgacgatgc cggcatacgg tttttgtggc tccttggcca cccgctctaa gcccagccgt
    12841 tcaaattcat cagcagggcc attcgccttg ttgatcggcc agagactcgt taaccggata
    12901 cgcaagctcg tcccttgccc ttgaatggct tcccccgtca tcagcagcat ttgtgccggc
    12961 aggggcaacg tcccctgctt cttccactgc tctgtcgcgg cggtcactcc gagttcctgc
    13021 atgcgttcca ccacgtgggt ggtatacagc gtgcggtccg cttgaacgat cgcatagacg
    13081 tactccgcca ccacctccgg tggaattccc gcttgccctt cagggcgcgc cgtttccccc
    13141 atgagtcctg cagaacacag caggctgagg ctgagcacga taacccctct tcgagtggcg
    13201 gtacgcatgg caactccctt accgaccagg tgtctcgctc gccgcgtgcg gctgcttctc
    13261 ttcagtcata ataatcagga gctcctggat ctccgtgccg ggcttcccat catggccgca
    13321 ccagatcaga tcatacattc tgcgaaatcc actgaagggt gccgtaaact gcaacgtgag
    13381 ggtgtcgccg ggattcacgc gaaagccggc agcatctttt cgaaaaatcc ccgtcgcacg
    13441 ccccaaaaac tggatctccg tcttggtgaa gagcggagag ataaactcac ggggaccgtg
    13501 atcttcgttc cggagcgcca ggacgatcgg ctcgcctgcg actaacgtca gcacttgtcc
    13561 gtgcgtttgt ggtcccttca ggatgggatg gcctccttct tgaacgacga tctcgacgtc
    13621 ggcggtcgcc ggcttggctg aggcatgagc gccttggtgg tgaccggacg cgccatcatg
    13681 cgccaaggtc ctatcagggg gaacgaggat gatggccccg gcgatgacgg ccatcactcg
    13741 agcgaaccgc agagcggaga aaagtgtgtc ggttggtttc attcaggctt cccgtcctcg
    13801 ttatccatgc ttgggcttca cgacaaagat ttccccttgc aggacatccc catggagctt
    13861 cccatggacg ttacaccaga acttgaacat gtccccgctg aagtccttcg gcaggtcaaa
    13921 gcgaagaccg acggtgtccc ccggatcgat gcggagacca ttggccgtat aggtgtagac
    13981 catcgtcgct ctgccccaga actggaattc caccttggcg aacaaggggg acacgaactc
    14041 atgaggcagc gtatcctcat tgcgcaactc cagcgtgatc tcctcccccg aagacaagga
    14101 gaaggccggg tgctccgagt gttgcccgga ggcatttccc ttgaccatat ggaaggcctt
    14161 ctccttgatg acgagaatca cgtcgggctc atactcacgc tcatcgcctt gagcaaaagc
    14221 gccgcgtgat ccacccgtca ggatcatcgc caggaccatg agcggcacaa gacaaacgtg
    14281 cacctcaacc tccgccaatc tcccctctgt gatcgacgac cagcacttcg ccgcgcatct
    14341 tgttgccgta ctgctttcca tggacgttgc accagaagac ctcatagaga ttttggaact
    14401 ctttggtgtc ctgaggaaca tcgaacgata accggaccac tcgtcccgga tccacccgca
    14461 ctccggccgc gttcggcaac ttcacaaaca ccccgttgcc atcgagccga aaggggacat
    14521 tgaacagcat cggggacacg aactcatggg ccacggtatc ttcgttcctg agcacgatat
    14581 cggctcgcgt acccgccaac agcatgaatc cttttgccgt ctctcctttg gcgatctgaa
    14641 acgactgccc cgtcatcacc acttcggcat tcggctccat attttctgac cgaatgattt
    14701 gaggacgacc ggcgtcggtt gcctcgttga ttgacgactg cgctgcagct ggggctgcaa
    14761 cagcaaggag cgcgatcacc gatgcgctta tgccgcctat gtgcctcatg attcctccct
    14821 tcgtgctaga gttgtcgctc attgacgcgt tcacccgctg aaccttccag tctcccacct
    14881 ccttggttgt agggagccaa tgttgctatg aaaggacgtg ctgctcctct ttcataccaa
    14941 tccctgcatt gcacatacct gcgaagcgaa gttgcgaatg gcctgaaaat ggagagaaac
    15001 gcgaaggaag gtcaaatacg tgggttatga ttgaatcgga ttcgatccgc tgtgcatccg
    15061 atccgatccg atcccatcga ttcttttctc gagggagcac tcagagcttt ttctgtctaa
    15121 gaaatttcaa caggacgatg gtgccgagga gtcgcgtctt tgaattcttg ggcttgagac
    15181 ttcccgtctt tactcaatag cgatctgagt gatgcgccat gtttcattca gggcctcgaa
    15241 gagctcacat caggacaaca aggtgagagc gccgacaatt cccc
//
LOCUS       DASSCQ010000015         8210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_993964, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000015 DASSCQ010000000
VERSION     DASSCQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993964"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VFH05_01035"
     CDS             <1..1344
                     /locus_tag="VFH05_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET6676830.1"
                     /translation="GVMVPCEQILAAAREQKADIVGLSGLITPSLDEMVHVAKEMTRE
                     GFDVPLLIGGATTSKAHTAVKIAPSYEPSVIHVLDASRAVGVVGNLMNREQKTPFVQG
                     VRQDYDRVRQAHLTREARPVLPLAEARARRLTSDWRTLHIPEPEFLGIRVIADQPLVE
                     LVPFIDWSPFFHTWELKGRYPGIFDHPTIGARAKELFTDAQDLLARMISEKLLLAKGV
                     YGIFTASSVGDDIELYADASKSTVVTTIHTLRQQSVKPDGQANLALADYVAPKESGRT
                     DHVGAFAVTAGIKLEELCSRFDRDHDDYNSIMAKALADRFAEAFAEFLHKRVRAEWGY
                     GRSEDLSNDDLIHEKYRGIRPAPGYPACPDHTEKRLLFDLLRVESNAGITLTESFAML
                     PAAAVSGWYFAHPDARYFAVGKIGKDQVEDYARRKGMEQETVERWLSPNLNYEPA"
     gene            complement(1355..1864)
                     /locus_tag="VFH05_01040"
     CDS             complement(1355..1864)
                     /locus_tag="VFH05_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676831.1"
                     /translation="MAKKALCVKKAGITRNWIAYLCEALVTSGVMPTWEAATYLTENL
                     FGESPNPRLMGTSNAYEVTAQFLHRLYQPVAEAAAREVSLPHGAMVHVFADISLTGKS
                     AVLVVYFPEGGQPHQLLMLDAAKAWDLVFQDSDTFNRWAEDRFRLLKSALTAAIGKPS
                     RQPLLAEVS"
     gene            complement(2033..3175)
                     /locus_tag="VFH05_01045"
     CDS             complement(2033..3175)
                     /locus_tag="VFH05_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6676832.1"
                     /translation="MTLLLDRPARQALLDLKRVLIIDDEEPIRRLLGYMLQSHGYETV
                     LAADAREARLRLDETPFALMLCDVNMPGESGMDLVRNIFRQHPHTAAIMVTGLDSPLL
                     ANAALEMGAFGYIIKPFESNEVLINVANALRRRRLEMENRLHRENLEDVVRTRTMALQ
                     QALEWLERSEKELRLSREETIQRLAIAAEYRDSATAQHIQRMSHYCELLARRHGLSTE
                     RCDLIRTASPMHDIGKIGTPDHVLLKPGKFTQDEFNVIAQHAEIGYRILAGSDAEILN
                     VAATIALTHHERWDGSGYPRKLKGEAIPIEGRIASIADAFDALTTARVYKPAFAFDHA
                     VDLMSKHRGEHFDPELLDVFLASLGDIKRIHEQYADRLTPGLPRQL"
     gene            3560..4303
                     /gene="rph"
                     /locus_tag="VFH05_01050"
     CDS             3560..4303
                     /gene="rph"
                     /locus_tag="VFH05_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET6676833.1"
                     /translation="MNPAASLVRADGRRREQLRPVKVTRNFIKHAEGSVLIEMGDTKV
                     ICTASIEEKVPPFLKGKGTGWVTAEYSMLPRATHDRSPREAVKGKQGGRTLEIQRLVG
                     RALRAVTDMTQLGERSIWIDCDVIQADGGTRTASITGAFVALADAMAILKTRGVIKKR
                     PLNDYLAAISVGKVGGEILVDLAYTEDCIAEVDLNLVMTGKGQFVEIQGTAEKTPFAK
                     QDMDAFLSQGWGAIQTLTALQRDLIGALE"
     gene            4306..4920
                     /locus_tag="VFH05_01055"
     CDS             4306..4920
                     /locus_tag="VFH05_01055"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HET6676834.1"
                     /translation="MARELVLATRNRHKREELAGLLDGLDIRIHTMDEFPDVPEVIED
                     GETCEANAIKKAHSISKATGLLAVADDTGLEVDALGGRPGVYAARYAGDHASYEDNWR
                     KLLGQLTGVPRHRRTARFVTVAAIVSPGETAQVATGYLDGLIAESPAGTHGFGYDPVF
                     FVPQVGKTLAEMSIVDKNRISHRAKAFAQVRNLLQNRLLRSVGA"
     gene            4911..4987
                     /locus_tag="VFH05_01060"
     tRNA            4911..4987
                     /locus_tag="VFH05_01060"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4945..4947,aa:Pro,seq:tgg)
     gene            5263..5667
                     /locus_tag="VFH05_01065"
     CDS             5263..5667
                     /locus_tag="VFH05_01065"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6676835.1"
                     /translation="MDSMPLILARLTVSGLVIINFLLLWLFVKQGSNGVQRRRMRRHP
                     IFLPVEVDGKKGVKGLINDLSLGGCRIGGTLPVKHGQHLPLRLHLPGQGSPIVIERAA
                     VRWVGEKDFGLQFMSIPSNERERLGELLQWVA"
     gene            6082..6369
                     /locus_tag="VFH05_01070"
     CDS             6082..6369
                     /locus_tag="VFH05_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676836.1"
                     /translation="MVLDERDRIHLNLQTIRDYLVKGFVGFQLTEDASDPTVCHRFTM
                     TDLKTFQQYKLKVSWSRLGELTNSAEKLAHSLVDTDVASKMREAKGHYWHW"
     gene            6528..6893
                     /locus_tag="VFH05_01075"
     CDS             6528..6893
                     /locus_tag="VFH05_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676837.1"
                     /translation="MLLLRKELHDYSRGCEALISAYLSPDTVAFTPKEIEWISHYAAE
                     ITSLVDGLVHRSLPAQHHERDTMREYVRVSDALLGLENLSDDERTSIRRAVNDIGKKI
                     LYASPLGAPPKWKQTLSDK"
     gene            complement(7470..8108)
                     /locus_tag="VFH05_01080"
     CDS             complement(7470..8108)
                     /locus_tag="VFH05_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676838.1"
                     /translation="MYSWGARRDMTTRFPLFFVAMTLCILVACSDQRPRPDNLLVEQG
                     QRAERLLEDSESGTAEDTYNLGMRFERGIVVRQNYPEAARWYRLSAMQAFPDAQYKLC
                     EFSEQGRGLPQDYQEALRWCGLAADQGHGPAMFMLGRLYHTGHGVPRDVVRAHMWYNL
                     ASAYGYEEGKRWRDRLAADDDALQMTRDQVAEAQKLAREWNVKMKATGHAGH"
BASE COUNT         1832 a         2220 c         2288 g         1870 t
ORIGIN      
        1 ggcgtcatgg tgccatgcga acagattctg gcagcggccc gtgagcaaaa agcggacatc
       61 gtcgggctca gcggcctcat tacgccatct ctggacgaaa tggtgcatgt ggccaaggag
      121 atgacgcgcg aagggttcga cgtgccgctt ctcattgggg gcgcgaccac cagcaaagcc
      181 cacacggcgg tcaagatcgc gccgtcatat gagccgtcgg tcatccatgt gctcgatgca
      241 tcccgcgcgg tcggcgtggt cggcaatctc atgaatcgag agcagaagac gccgttcgtg
      301 caaggggtcc ggcaggacta cgatcgtgtg cggcaggctc atctgacccg ggaagccaga
      361 cctgtgctcc cgctcgcaga ggcacgcgcg cgccgcctta cgtcggattg gcggacactt
      421 catattcctg agccggagtt cctcggcatt cgggtcattg ccgatcaacc gctcgtcgaa
      481 ctcgtgccgt tcatcgattg gtctccgttt tttcatacct gggaactcaa gggacgctat
      541 ccgggcatct ttgatcatcc gacgatcgga gcgagagcca aggagctctt taccgacgca
      601 caggatctct tggcccgaat gatctctgag aaactgttgc tcgccaaagg tgtctacggc
      661 atcttcaccg cctcgagcgt aggtgacgat atcgaattgt atgccgatgc ctcgaagagt
      721 accgtcgtga cgaccatcca tacattgagg cagcagtcgg tcaaacctga cgggcaggca
      781 aacctcgcac tggctgatta tgttgctccg aaagaatccg gacgaaccga ccacgtcggg
      841 gcctttgccg tcacggcagg aatcaagctg gaggagctgt gcagccgttt tgaccgggac
      901 catgacgact acaactcgat catggcgaag gcgctggccg accggttcgc cgaagcgttc
      961 gccgagttcc tccataagcg ggtaagagcc gaatggggat atggcagatc ggaggatctg
     1021 tcgaacgatg atctcattca cgaaaaatat cgagggattc gccctgcgcc cgggtatccg
     1081 gcctgtcctg accataccga aaagcggctg ttattcgatc tcctgcgtgt cgaatccaat
     1141 gccggcatca ccttgacaga aagcttcgct atgttaccgg ccgcggcggt cagcggctgg
     1201 tatttcgcac acccggacgc gcgctatttt gcggtaggca agatcggcaa ggaccaggtt
     1261 gaggattacg cgcgtcggaa ggggatggag caagagacgg tggagcgctg gctgtcgccg
     1321 aaccttaact acgaacccgc ctgatgcttt ccggttaaga gacttccgcg agaagcggct
     1381 gccgggacgg tttgccaatg gccgcagtga gtgccgattt gagcagtctg aagcgatcct
     1441 ccgcccaacg gttgaaggtg tcagaatcct gaaagaccag gtcccaggcc tttgcggcat
     1501 ccaacatcag caactggtgt ggctggccgc cttccgggaa atacacgacc aggacggccg
     1561 attttccggt caaactgata tccgcgaaga catggaccat tgcgccgtgt gggagcgaaa
     1621 cttcacgtgc agccgcttcg gccacaggct ggtataagcg gtgaaggaac tgcgcagtga
     1681 cttcataagc gttgctggtt cccatgaggc gcgggttcgg agattctccg aagaggtttt
     1741 cagtcagata ggtagccgcc tcccacgtcg gcatgacccc cgacgtcacg agtgcttcac
     1801 acaggtaggc aatccagttt cttgtgatac ccgctttctt cacgcacaaa gctttcttgg
     1861 ccatgatcgg caatcctttc gatgccagcg tgacggcgtg tcaccggtgc ctctgtcaga
     1921 ggacaaactt ttgaactcat tggcagtata gcgacgaccg gaccacagtc aattaaatgc
     1981 actaaactga cgaagcgatg cggagacttg aacgatgggg agcgatgtcg acttataatt
     2041 gacggggaag gccaggagtc aggcggtcag cgtactgctc gtgaatgcgt ttgatatccc
     2101 ccagtgaggc gagaaagaca tcgagcagct cgggatcgaa atgctcgcca cggtgtttac
     2161 tcattaggtc cacggcgtga tcgaatgcga acgccggttt gtacacgcga gcggtagtca
     2221 gggcgtcgaa ggcgtcagca attgaggcga ttcgcccttc gatcggaatg gcctctcctt
     2281 tcaacttccg gggataccca cttccatccc agcgctcgtg gtgcgtcaac gcaatcgtcg
     2341 ccgcaacgtt caaaatctcg gcatcggatc cggccagaat gcggtatccg atttcggcat
     2401 gttgggcgat cacgttgaac tcgtcctgtg taaacttacc gggtttgagc aacacgtgat
     2461 cgggcgtgcc gattttacca atgtcgtgca ttgggctggc ggtccggatc aagtcacagc
     2521 gttcggtgga caatccgtgg cggcgtgcga gcagttcaca ataatgactc atgcgttgga
     2581 tgtgctgggc ggtggcgcta tctcgatatt ctgccgcgat ggcgagccgc tggatggttt
     2641 cttctcgcga cagccgtaac tccttctcgc tccgttccag ccattccaac gcctgttgca
     2701 gtgccatcgt cctggttctg acgacgtcct cgaggttttc gcgatgcaga cggttctcca
     2761 tttcgaggcg ccgacgccgg agagcattcg cgacattgat aaggacttcg ttggattcaa
     2821 atggtttgat gatgtacccg aatgccccca tctccaatgc ggcgttggca agaagcggac
     2881 tgtccagccc cgtcaccata atcgctgccg tgtgaggatg ctgacgaaag atgttgcgga
     2941 ctaaatccat gccggattct cccggcatgt tgacatcgca tagcatcagc gcgaagggtg
     3001 tttcatccag ccgtaagcgg gcctctctgg catcagcggc caagaccgtc tcatacccgt
     3061 gcgattggag catgtaccca agcaggcgcc ggatcggctc ttcgtcgtcg ataatcaaca
     3121 ctcgcttgag atcgagcagt gcttgtcggg cggggcgatc gaggagcaac gtcatggctc
     3181 gatgatatct cgggtcaatg aggttcgttg tgtcgtcggt ctggatccga tcgtctcgtt
     3241 gagaactcta gaacgaggtg caccttttgt gccactaatt agtgattttg caccgactta
     3301 ggcggcagtt attacggatc gattcaccgg acccacaccc gcgaaaacgt gtgagttcgc
     3361 catccgaact agtgatagca tccgtgcgaa gcctgctgtg caccatccca gtcgctgtac
     3421 aatctcgtgg ctaccagtca aattctgtac ggtaaggcat acggatctat ctctgtcttc
     3481 cggtgcagat cgcagagcgg tttcattgcg atttggaaat cgcttcacta taatgcccca
     3541 atttcttaag ggagatttca tgaacccagc agcgagtttg gtacgcgcgg acggccgccg
     3601 gagggagcag ctccgccccg tcaaagtgac caggaacttc atcaagcatg ccgaaggatc
     3661 ggttctcatc gagatgggcg ataccaaggt catctgcaca gcttcaattg aggaaaaagt
     3721 gcctcctttc ttgaagggaa aagggacggg atgggtcacc gctgaatact ccatgctgcc
     3781 gcgcgccact catgaccgct cacctcgtga agcggtcaag ggcaaacagg gcggacggac
     3841 actggaaatt caacgactcg tagggcgtgc actgcgcgcg gtcacggata tgacacagct
     3901 cggcgagcgt tcgatttgga tcgactgcga cgtcattcaa gccgacggcg gcacgcgtac
     3961 ggcctccatc accggagcct ttgtcgcgtt agcggatgcc atggctattc tcaagacccg
     4021 cggtgtgatt aagaagcgtc cgctcaacga ttatctggcg gctatcagcg tgggaaaggt
     4081 tggcggagaa atcctcgtcg atttggcgta tacggaagat tgcatcgcgg aagtagattt
     4141 gaatctggtg atgaccggaa aaggtcagtt tgtcgagatt cagggcactg cagagaagac
     4201 gccgtttgcc aaacaggata tggatgcatt tctttcacaa gggtggggcg ccatacaaac
     4261 cctgaccgca ttgcagcggg acctcatcgg cgcgttggag tagccatggc ccgtgaactg
     4321 gtcctggcta cccgcaatcg gcacaaaagg gaggagttgg ccggactgtt ggacggtttg
     4381 gacattcgca tccatacgat ggacgaattc cctgacgtgc ctgaggttat cgaagacggg
     4441 gaaacctgtg aggcgaacgc gatcaaaaaa gcacactcga tttcaaaagc gaccggtctc
     4501 ttggctgtcg cggacgacac ggggttggaa gtggatgcat tgggtggtcg acccggtgtg
     4561 tatgcagctc gctatgcagg cgaccatgcc agctacgaag acaactggag gaagctgtta
     4621 gggcagctca cgggtgtccc gcgccatcgg cgaacggccc gcttcgtaac ggttgccgcc
     4681 atcgtgtctc cgggagagac cgctcaggtc gcgaccggtt atcttgacgg tctcatcgct
     4741 gaatcaccag ccggaactca cggttttggc tatgatccgg tctttttcgt gccacaggta
     4801 ggtaagacgc tcgccgaaat gtcgatcgtc gacaaaaaca ggatcagcca tcgcgcgaaa
     4861 gcctttgcac aagtgaggaa cttgttgcag aataggttgt tgcgatcagt cggggcgtag
     4921 cgcagcccgg tagcgcgcct gctttgggag caggatgtcg gaggttcaaa tcctctcgcc
     4981 ccgaccaagt ttcagttacc tgccacttct ctcgtccaca ttagtttctg atcccttctt
     5041 ctaccccggc cacaggcagc tcgcagtgaa aatatgattt attggccgtt cgtgtccccc
     5101 cttccataca gttcctgtac accgcattgc cctatccgaa ccttgcagac aattgtttca
     5161 tgagcttaag gatggcatgc cgattgcgtt gtcgacgtct cctgtgcatt gaacgaaaac
     5221 tgcgcagcac gaagacaaca gtcactaatc tggaggaggg tcatggattc gatgcctttg
     5281 attctagcgc ggctgactgt gtcaggactg gtcattatca attttctcct actgtggctc
     5341 ttcgtgaagc agggttcaaa cggagttcag cggcgccgga tgagacggca ccccattttc
     5401 ctacccgtgg aggttgatgg aaagaagggt gtgaaggggc taatcaacga tctttccctt
     5461 ggaggctgca gaattggcgg tactctgccc gtcaaacatg ggcaacatct cccattgcgg
     5521 ctccatctac ctgggcaggg atcgcctatt gttattgaac gagcggcggt acgatgggtg
     5581 ggcgaaaagg actttggcct ccagttcatg tcgataccct ctaacgagcg agaacgactg
     5641 ggggagttgc ttcagtgggt ggcataagtg gcagtgagaa cgacagaact aatcgacata
     5701 tttaaaccct ccgattgccc ccgagatatg cccagacagt tgattcatca agaattgctg
     5761 ctcaggccag ttcaaggcct cataaactcc aagccaaatt gttcgaccat cccacggcag
     5821 ttagcttgac tgaaacgtca ggtccgacag taatatctga agtcggccta ttgcgttttt
     5881 agggcgctct gtcacctttc cgattagcct acagcgtccg agtttgaggg gaactccttc
     5941 tcatgtacgc cttgtattgc ctcagctaag tggcagacgc tggcaattct cactacggcc
     6001 acaaggccct attgaattca ttaatactgt tgtatggttc tgcatgaaca cgtgcagtga
     6061 aacctcagat aaggtgcgtt tatggtgctc gacgaacgtg atcgtatcca cttaaatctc
     6121 cagaccattc gggactattt ggtcaaggga tttgttggat tccagttgac cgaagatgct
     6181 tccgatccga ctgtctgtca caggtttacg atgacagatc tgaagacttt ccaacagtac
     6241 aagctaaaag tctcatggtc acgccttggt gaattgacaa acagtgcaga gaagctggct
     6301 cattcactgg ttgatacgga cgtagccagc aagatgcgag aggcgaaagg tcattattgg
     6361 cattggtaaa agcatagaac cttttcttca attagatagc cgcttcatcg cgggttgaat
     6421 caaattgacc tggccttcca cagtaccgta gactcgacct ccagttgtcc caactgcgga
     6481 tggccccatg gatcgccacc cccgactcgt gagaaaggcg tactgcgatg ttgcttctgc
     6541 gcaaagaact gcatgattat agccgcggct gcgaggcatt aatttcagcc tatctatctc
     6601 ccgataccgt cgcctttact cccaaagaga tcgaatggat ctcacattat gcggcggaga
     6661 taacgagcct tgtggacggc ttggtgcaca ggtctttgcc tgcgcagcat catgagagag
     6721 acacaatgcg agaatatgtc cgtgtgagtg acgccttgct cgggctagaa aatctttctg
     6781 atgatgagcg aacaagcatc cggcgtgctg tcaacgacat cggtaaaaag atcctgtacg
     6841 catccccctt gggtgccccg ccaaagtgga agcagacttt atcggataag taattcggtg
     6901 attattcgcc gtgttctgtc cgtatcatga aacggggtca cgcgtctttg agcccatcca
     6961 caaaggcaag cctccgattc tcgtccgtaa aaccaaaaag gcggggttgt tttcctagag
     7021 acggagaaaa gatatgctag cccagtggat ggtttcatca tcggtgatat gcccagcagc
     7081 cgtttctatt ggctttgccg tcaccttatt ctctcttccc gttcaggcgc aaatgtaccc
     7141 tcaggacatc catgcccaca gccgcaacat aggacctagc gctccaactc aatctttttt
     7201 tggtcggggt ggcgtctacg ataccgcctc tgagcttctc cagcgccggg aagaagaaga
     7261 acgagaacgg cgagaaatac gggagcgcca gcgccggatg ctagagaatt ttacgaatat
     7321 ccctgatcag cttcggcagt tcgatatggg tcgagttgac cctcccttct ctcgacgtta
     7381 gagagttcct tcgatgtccg ccgaatgagt cagcccgtat cttgcgggga cttgttggct
     7441 tcctgcggtt tgaatggcga tgaactgctc tagtggccgg cgtggccggt cgccttcatc
     7501 ttcacattcc actcacgtgc caacttctgc gcctcggcaa cctggtcgcg agtcatctgc
     7561 aaagcatcat catcagcagc cagacgatca cgccatctct tcccctcttc ataaccatag
     7621 gctgacgcca ggttgtacca catatgcgct cgcacaacat cccgaggcac accatgccca
     7681 gtatgataaa gacgtccaag cataaacatc gcaggcccgt gaccctgatc agcggctaga
     7741 ccacaccaac gtaacgcttc ttgatagtcc tgtggcaagc cgcgtccttg ctcggagaac
     7801 tcgcaaagtt tgtattgagc atccggaaac gcctgcattg cagagagccg ataccaccgg
     7861 gcggcttctg gatagttctg ccgcacgacg attcctcttt cgaatcgcat gccgagattg
     7921 tatgtgtcct ctgcagttcc actctcagag tcttcgagaa gcctctcggc ccgttgacct
     7981 tgctctacaa gcagattatc aggtcgtggc cgctgatccg agcaggctac gaggatgcat
     8041 agggtcattg ccacgaagaa aagtgggaaa cgcgtcgtca tgtccctcct tgctccccaa
     8101 gagtacattc acggactctc ggagtgtcat tcaacattga tagcgtagcg tgtttgattt
     8161 taataccgcc tgggttggca ataaagcttc gggcgaagaa tcaatacctt
//
LOCUS       DASSCQ010000016         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1433387, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000016 DASSCQ010000000
VERSION     DASSCQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433387"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..678)
                     /locus_tag="VFH05_01085"
     CDS             complement(100..678)
                     /locus_tag="VFH05_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6676839.1"
                     /translation="MKPTHHTSTIWRSSGGFTLIELTICLAIVAVLASLALPIHLEAV
                     EGAKAVEATSALTEVVRLERLRFAETGTYTADLQELGFQLVHSLQYTELFVEVRKDAK
                     GWSYRAFAIPRGGQNSHQIRGVTSRADGSFDMSFPGTLKSGGGSACSVWTGWGSMEGG
                     RIEGEETLSASSSTGGSGSPCGGRRVVDHGKQ"
     gene            complement(744..1859)
                     /locus_tag="VFH05_01090"
     CDS             complement(744..1859)
                     /locus_tag="VFH05_01090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6676840.1"
                     /translation="MKPGTGMMDVTGMMKPTSSRVYLVLLHVMFLVLYGAHLTWPLTR
                     LQGGLIIALAVLTLLVLTVPRTQLSTAWFLGLLTLGNASILFATTMIERVSAGPWILG
                     ALMLVLAMASYVTCLFHFALLNGLVIGSYAWLLHYMSLLQTENVLLLPALLCLTLAFL
                     SKISLTQAEIRRLTETEEYTRSKSLCDALTGLPNRAQFLERVGRAVQCQQQNRDMHFA
                     LLFVDLDGFKPINDRLGHKAGDAVLRQTARVFQGCLRKGDIVARYGGDEFTFLLNHVK
                     GPSDAIRVAERILAKVQTPIDVGEPVQVGASIGIALSTNLHERPEDLIRDADGAMYRA
                     KAQGKQCFVLSDQAADVSTNELKARWKRLTHLGWNRV"
     gene            2086..2445
                     /locus_tag="VFH05_01095"
     CDS             2086..2445
                     /locus_tag="VFH05_01095"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6676841.1"
                     /translation="MQNKQGQEGSGQAMTGEAERRGRRLPLNRRLFFFGEEEFEGEAT
                     VLDISTNGCRATSLTEVKSGLVLKLSLFLQDQQWPLRVDQAVVRWVEGQCFGLEFISI
                     RPAQRERLRSILMKAKA"
     gene            3083..3496
                     /locus_tag="VFH05_01100"
     CDS             3083..3496
                     /locus_tag="VFH05_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6676842.1"
                     /translation="MPEFMHPYTSQTYALMRIVTGFLFLWHGTQKLLSIPTPPPPQVP
                     TFVIAVAGPIELIGGTLVMIGLFTGWAAFLCSGLMAFAYWMGHGTQALLPIQNMGELA
                     ALYCFVFLYISARGSGIWSVDAARKHPSSVSLHAK"
     gene            complement(3600..4004)
                     /locus_tag="VFH05_01105"
     CDS             complement(3600..4004)
                     /locus_tag="VFH05_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676843.1"
                     /translation="MAVDVRIIPIKEFLRTDVSGNLDLEKSLIILGDLVEESKKNNVD
                     RILIDTREATSNASMLDVWTLARKLTNSGLRAQIAVVNKPKDDFDRGAFLELCAMNRG
                     YQLKAFRDFEAAFTWLNEDQPSPSSGSTRYGI"
BASE COUNT          925 a         1173 c         1147 g          935 t
ORIGIN      
        1 gtagcaacct ggtagcagac gatctagtat tgaaatagca taactttgag aggcaccaaa
       61 ctatttaacc gcaggtcaac tgaaccgtca ctcactcttt tattgtttcc cgtgatccac
      121 caccctcctt cctccacaag gcgaccccga tccacctgtt gaggaactcg cactgagagt
      181 ctcttcgcct tcaattcgcc ctccctccat ggatccccat ccggtccaaa cgctacaagc
      241 ggatccaccg ccggatttca atgttccagg gaagctcatg tcgaaactcc catcagcccg
      301 gctcgtgacg ccccgaattt gatgagagtt ttggccacct ctgggaatgg caaaggctcg
      361 gtaactccag cctttggcat ccttgcgcac ttcaacaaag agctcagtgt attggaggga
      421 atgaacaagc tggaatccca attcctggag atccgctgtg taggttcctg tctctgcgaa
      481 acgcaagcgc tccagtcgaa ccacctccgt cagggctgac gtggcctcga ccgctttggc
      541 tccttccaca gcctccagat gaatcgggag agccaggctg gccagcaccg caacaatggc
      601 cagacagatg gtcagctcga tcagggtgaa tccgccactg ctccgccata ttgtcgatgt
      661 gtggtgagtc ggtttcattg gtggttccat gctgtttctg ttgcggtctt cgaaaccgca
      721 atgaattctt cgggtcctcc tgcctagacc cggttccatc ccaggtgcgt gagtcttttc
      781 caccgcgctt tgagttcatt ggttgacacg tccgcggctt gatcgctgag gacaaaacac
      841 tgtttgcctt gcgccttcgc ccggtacatg gcgccgtcgg cgtcacggat caaatcttca
      901 ggacgttcgt gcaggttcgt gctcagggca atgccgatgc tcgcgccgac ctgtaccggt
      961 tcaccgacat caatcggcgt ctgcactttg gcgaggatgc gctccgccac acgtatcgca
     1021 tccgagggac ctttgacgtg gttcagtaag aaggtgaatt cgtctccacc atagcgtgca
     1081 acgatgtcgc ccttcctcaa acacccttga aacactcgtg ccgtctgtcg gagcacggcg
     1141 tcgccggctt tatgtcccag ccggtcattg atcggcttga acccatccaa gtcgacaaac
     1201 aagagcgcaa agtgcatatc gcgattctgc tgctgacact gcacggctcg accgacacgc
     1261 tccagaaatt gcgcgcgatt cggcaatccc gtgagcgcat cgcagaggga tttgctgcgc
     1321 gtgtattcct ccgtttcagt cagacgccgt atttcggctt gcgtgaggct gatcttgctc
     1381 aagaacgcaa gggtgaggca caacaaggcg ggcaggagga gcacattctc cgtctgcagc
     1441 aggctcatgt aatgcagcag ccacgcatag ctgccgatca ccaggccatt caataaggca
     1501 aaatgaaaca gacacgtgac ataggaggcc atcgcgagca cgagcatcaa ggcgccgagg
     1561 atccaggggc cggcgcttac cctctcgatc atggtcgtgg caaatagaat gctggcattc
     1621 cccagggtca gaaggccgag aaaccatgcc gtgctgagct gggttctggg gacggtgagg
     1681 accaagaggg tcagaacggc cagcgcgatg atcagtcctc cctggagccg tgtcaggggc
     1741 caggtaagat gagcaccgta gagcacgagg aacatcacgt ggagcaggac gagatagact
     1801 cgtgagctgg ttggcttcat cattcccgtc acatccatca ttcccgtccc aggcttcata
     1861 caccaagcct acccggtgcg ttgagagggg acattcccag gaagggggag gaaaacggct
     1921 acgaacgtag ggaagggggg gtatgagaaa gtacgtagct tcggtaaact gacccgcatg
     1981 tcggccgaca cccgttcgtt gggagtactc ctcttgagga atgcgtgtaa cggagtgatc
     2041 tcataccatt gagtgagaac actgagtttc gcgattgaag gcacgatgca gaacaagcaa
     2101 ggacaagaag gttcgggcca agccatgacg ggcgaagcgg aaaggcgtgg tcgccggctg
     2161 ccgctcaatc gccgtctctt ctttttcggg gaggaggaat tcgagggcga agcgaccgtg
     2221 ttagatatct caaccaacgg ctgccgtgcc acgtcactca ctgaggtcaa gagcggctta
     2281 gtgctcaaac tgtcgctatt tcttcaagac cagcaatggc ccttgcgggt cgatcaggcg
     2341 gtggtgcgat gggtcgaggg ccagtgcttt ggtctggaat ttatcagcat taggcccgcg
     2401 caacgcgagc gattgcggag catcctcatg aaagcaaagg cctgaccgta ctggccatca
     2461 ccccgatcat ttctccttct ggacacgtcg cttccttcgc cagaagcctt aacttcgacg
     2521 atcaattcac cacgacaccg tgtggtcggt tctatgaaga tcagcgctgc aggcgaaaac
     2581 cgtctgggtt tccggacctc cgctcctcga gtcgccgctg tgaatccgcg agcttggcga
     2641 ccgcattggc gtaaaaacag atttgcttga gatcctcgtt cgagaacggc atatggtccg
     2701 acgcttgagc gctgtgtaag agtcgctcgc agtatcggaa aaaaccctga aggtcttcgc
     2761 gggcggaggg aagtggcgag ctggacatag gacgaccgcc tttcaggtgt ctggcggtcg
     2821 ggccttccca ggaggccgca ggcacaggtg cagggtgaga tgatgtaatc taggctctct
     2881 ggaagactgg gtcaagcggt cattcgaact ggatgcatac ggattgttga agcgatggag
     2941 gcgactccaa tcatgttcat cgggcagcct tgacgcacac tgaaagcacc gtgttgacag
     3001 cctggattga agccagtagt cttccctcgc tccgagtgcc ggatgcttta ccggtatccc
     3061 acctgtgtag aaggaggact cgatgcccga gttcatgcat ccctatacct cccagaccta
     3121 tgcgctcatg cgaatcgtga cgggatttct cttcctctgg catgggactc aaaaactgct
     3181 gagcattccc accccgccgc cgcctcaagt ccccaccttc gtgatcgcag ttgcaggtcc
     3241 gatcgaactc atcggcggaa cgttggtcat gatcgggctg tttacaggat gggcggcatt
     3301 tctctgcagc gggctcatgg cattcgccta ttggatgggc catggcacgc aggcgcttct
     3361 ccctattcag aacatgggtg agctggccgc tctgtactgt ttcgtgttct tgtatatttc
     3421 tgcgcgaggc tcaggcatct ggagcgtgga cgctgcacga aagcatcctt ctagtgtctc
     3481 acttcacgcc aaataggatc tggagaaagc gcccggaagc agtacagtag gcgactgccg
     3541 gattcaccat gagaggttca cattattgag ctgaagtcag cgacattgga atgtaagcat
     3601 cagattccat accgtgtcga acccgatgaa ggggatggtt ggtcctcatt gagccatgta
     3661 aaggccgctt caaaatcccg gaaggctttc agctgatacc ctcgattcat ggcgcaaagc
     3721 tcaagaaacg ctcctcgatc aaaatcgtcc ttcggcttgt tcaccaccgc gatctgggcc
     3781 ctgagcccgg agttggttag ctttcttgcc agtgtccaca cgtccagcat gctggcgttc
     3841 gaagtcgctt ctcgagtatc gataagaatc cggtccacat tgttcttttt ggactcttcc
     3901 accaaatcac ccaggataat gaggctcttc tccaaatcca gattacccga cacatcggtg
     3961 cgcaggaatt ctttgatggg gattattctc acgtcgacag ccatagttca ccccgctgca
     4021 ttatatagcc aataaccgcc acagtacgtc atcgatagct tctgtcgccc agcaagatag
     4081 cagacattct gaccactctg ctgtcgtagg cgaatgaatg atgtctctat agcgtattca
     4141 acaggtggac cgcaacttgt gaagcgaatc ctgttgtagt
//
LOCUS       DASSCQ010000017         7338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1853603, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000017 DASSCQ010000000
VERSION     DASSCQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853603"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2948)
                     /locus_tag="VFH05_01110"
     CDS             complement(<1..2948)
                     /locus_tag="VFH05_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6676844.1"
                     /translation="MHDRAAQDLRPGSTTIVLYPKYRTILEREYMEFRPFGVDEQGKK
                     IRDLTGMSIRATVLYLESFITRRHGEAAGLQAVQDLCASLNGRIKNPVYHVTPAFLKN
                     AWNSYSYEFTAYLYEFCEQISGDDQFVYRGGREKASPIMQVLARPFSLSQIYGMFPYF
                     GNKFASGSIECRVVGVTNRSATLAMRFSERTLRQFGPYRRRCTFVMCQAAQGIMAAVP
                     ARVHGLPPATLTECSCIANDDDWCQWDITWHAEPTGGWARRIWSRGGSSPAMPEGGPP
                     ILSSDQMDALLPSAGLQPDAKVAQDHHPLRFHDVAPSLPDSSPQKHALSRLPGSKSKT
                     DIAGYVWALSSAALTSSIVHLVYPAMSLGEVLLAGLIPVLVAGSWINWRLRKESQRRE
                     CLIQEQITFVESRHEELREAYLEQEHTRVELRRTVNHLTALHQAGLLFSSTLDREALL
                     EKVLETLIHDLHFDRAMISFYDPVRRMTTDARVAGVSREVQDLARMHETPVGDPESLE
                     GVVLLQGKPLLIGDVQPYVARMHPHNRRLLQLTGSKSLLAVPLKTKDRILGSMIVDRM
                     QEHGLTQDDLELMVTFAHQVAIALDNASAYQQIEELNVGLEAKVQERTRELEQADRLR
                     SQFLSHVSHELKTPMTSIKGFLQNMLDGLTGSINEKQHRYLSRMLENSERLIRMIEDL
                     LDRTRIQTGKLELAPVELDLGQCVADVMEQLRPLVQAKRQMLEATYPDVPLVVWADRD
                     RLVQIVINLVQNAMKFTPEEGRITVRVGKDNHRHAGVAVLDTGPGIAPEFRDKIFDPF
                     FRITQTRSSAKGLGLGLSIVRTLVELHGGTIEARNSDGKGAELYFTLPLRYPVEGSVP
                     TVSKRAPRILVVDDDKDIRQLLDDRLVAMGYEVETETDGLRALKAIGTGRFSGVILDI
                     GISSVDGMDVLKQIRQDDAHIPIIMVTASGAKESAIRAMSLGAQAYLLKPFDVEELRQ
                     VMESYFRTAE"
     gene            complement(3025..3333)
                     /locus_tag="VFH05_01115"
     CDS             complement(3025..3333)
                     /locus_tag="VFH05_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HET6676845.1"
                     /translation="MVLLEFSMSPLGKGESVSKYVSRSLDIIDKSGVAYRLNPMGTVL
                     EGEWDEVFAVVRKCYERMKKDCNRISCSIKVDYRKGHSGRLVSKVASVEKTLKRSIRQ
                     "
     gene            complement(3529..4173)
                     /locus_tag="VFH05_01120"
     CDS             complement(3529..4173)
                     /locus_tag="VFH05_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HET6676846.1"
                     /translation="MAANPGFPLTLDVKGYVVLVLGGDEEAAEKTQRLLDAGAKVIVI
                     APTLHEQLKQLAASAKIIHRGRHFRETDLESAVLVLNMLRGDRDFARSLFAKARERKF
                     LLWSVDFPEASTVNMPAVVEAGHARVAISTSGMAPALSGFMKEDLERILDAEFVAFVE
                     WLGELREQAKSNEPDVEKRRTMLREALDGFRLHGKVQYPKVWLEERDKTKARTG"
     gene            4309..4764
                     /locus_tag="VFH05_01125"
     CDS             4309..4764
                     /locus_tag="VFH05_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676847.1"
                     /translation="MAAYKFGAVGMAGGIIALAAVAAWLGLAHTCDPQGGEAVIAGQV
                     VISPGLADHVRPTDVLFVIVKRPGGTPRPVAAKRIESPKFPVPFEITNADVMVQGSEL
                     RGMVDVMARLDRDGQAGPAQPGDIEGRYAKNPTLPGGRDLEIILDTVKQ"
     gene            complement(4867..5409)
                     /locus_tag="VFH05_01130"
     CDS             complement(4867..5409)
                     /locus_tag="VFH05_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET6676848.1"
                     /translation="MEMTLLLNSTYEPLRVVHWQKAIALLWQGKVEVLEVYDREVHGV
                     SISFKLPAVMRLLKLVRLKDSHRAVKFSRINIFTRDGYVCQYCRTKFRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNRKSGRTPDEAGMRLMKRPVKPRWSPIVTITIGIRNT
                     PESWRDYLYWNMELDADSGP"
     gene            5575..5895
                     /locus_tag="VFH05_01135"
     CDS             5575..5895
                     /locus_tag="VFH05_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6676849.1"
                     /translation="MSKFITVAKTQDVPPGTGRTVEVQGVWIALFNVDGSFFAVDNTC
                     PHAGGPIGEGSLSGDVVTCPWHGWQFNVRTGERPGNPNFTVACCPVRVEGEQIQIALP
                     PDFK"
     gene            complement(5902..6357)
                     /locus_tag="VFH05_01140"
     CDS             complement(5902..6357)
                     /locus_tag="VFH05_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676850.1"
                     /translation="MVKILHYVVLGVILLGVVYYIWTKPPTINPMIDRRSADALAMVQ
                     THRALGYPTILQAMTEHVRSMKDRGLNARLGEWRVKQVDGDLYEVRVQLRDKGVGGQW
                     FEREFIWHANLSLKRVNAASLPADGITPKPAENEEPAMPAPKPNILGPA"
     gene            6606..7049
                     /locus_tag="VFH05_01145"
     CDS             6606..7049
                     /locus_tag="VFH05_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET6676851.1"
                     /translation="MKFFLYADNLHPTQLKRRAPEHQFQMLATLPDHTIQFCRWSSQW
                     RCGLASVVPSPGEKVWGAIFELTDEDIKLLDQFEDDVPQEAFRHVQVTVLSETGEKIL
                     VTTHAATPIGKFKPKEHYLDWVIKGLKHWKFPDEVVEQWQAYRPH"
     gene            7118..>7338
                     /locus_tag="VFH05_01150"
     CDS             7118..>7338
                     /locus_tag="VFH05_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET6676852.1"
                     /translation="MPKPKYHIVVCTNARPPGHPKPSCGSAGSAQLLMAFNMGLMQRG
                     VMPGEVLVSGSSCLGPCEQGPTVVVYPEGT"
BASE COUNT         1538 a         2193 c         2060 g         1547 t
ORIGIN      
        1 tactctgccg ttcgaaaata cgattccatg acctgccgta gttcctccac gtcgaacggc
       61 ttgagcagat aggcctgtgc accaaggctc atggctcgaa tcgcggattc cttcgcgccc
      121 gaagcagtga ccatgatgat ggggatgtgc gcgtcgtcct gtctgatttg ctttaaaaca
      181 tccattccat cgacagagga gatgccgatg tcgaggatca caccggaaaa ccggccggtg
      241 ccgatcgcct tcaaggccct aaggccgtcg gtttccgttt ccacctcata tcccatcgcc
      301 accagacggt cgtccagcaa ctggcggata tccttgtcgt catcaacaac gaggatacga
      361 ggcgctcgtt tggatacagt ggggacactt ccttcaacgg gatatctcaa cggcaaggtg
      421 aaatacagct ccgctccctt tccgtcagag ttgcgcgctt caatcgtgcc tccatgcagc
      481 tcgacgagcg tgcgcacgat cgacaaaccc agacccagcc ctttggcgct cgacctcgtt
      541 tgcgtaatcc tgaaaaacgg atcaaaaatc ttgtccctaa attccggggc aataccgggc
      601 cccgtatcga ggaccgccac gcctgcgtgc cggtgattgt cctttccaac cctcacggtg
      661 atgcgcccct cttcgggcgt gaatttcatc gcgttctgaa ccagattgat cacgatctgg
      721 acaagccggt cgcggtctgc ccatacgaca aggggaacat ccggataggt ggcctcgagc
      781 atttgccgtt tcgcttggac caacgggcga agctgctcca tcacatcggc tacgcactgg
      841 ccaagatcca gttcgacggg agccagttca agcttgcctg tctgaattct cgtccggtcc
      901 aacaggtctt cgatcatccg gatcaggcgt tcggaattct ccagcatccg ggacaggtat
      961 cggtgctgtt tctcattgat cgatcccgtg aggccgtcca gcatgttctg aaggaagccc
     1021 ttgatcgagg tcatgggcgt tttcagctcg tgcgatacgt gcgagagaaa ttgcgaccgc
     1081 aggcggtcgg cctgctcgag ttccctggtc cgttcctgca ctttggcttc caagcccacg
     1141 ttgagctctt cgatctgctg gtaggccgac gcgttatcga gcgcaatggc gacctggtgc
     1201 gcgaaggtga ccattaattc gagatcgtcc tgcgtgagcc catgctcctg catccggtcc
     1261 acgatcatgc tgcccaagat acgatccttc gttttcaggg gtaccgccag gagcgacttg
     1321 gagccggtca actggagcag acgtcggttg tgcggatgca tgcgggccac atagggctgc
     1381 acgtcgccga tcaagagcgg tttgccctgt aagaggacga cgccctcgag cgactccgga
     1441 tcgccaaccg gcgtctcgtg catcctcgcg aggtcttgaa cctctcgtga gactcccgcc
     1501 acacgcgcat ccgtggtcat tcgacgaacc ggatcgtaaa acgagatcat cgcccggtcg
     1561 aagtgcagat catgaatcaa tgtttccaag accttttcca gaagggcctc gcggtcaagc
     1621 gtcgaactga acagcaagcc ggcctgatgc agcgccgtga gatgattgac cgtgcgccgc
     1681 agctcgaccc tcgtgtgttc ctgttccaga taggcttcgc gcaactcttc gtgacgggac
     1741 tcgacgaagg tgatttgctc ctgaatcaag cactcgcgcc gttggctctc ctttctcaac
     1801 cgccagttaa tccagcttcc ggcaaccagg acagggatca atccagccag caagacttca
     1861 cctagactca tggcggggta gacgagatgt acgatgctgg aggtgagcgc ggcgctcgag
     1921 agggcccaga cataccccgc gatgtctgtc ttggacttgc ttcccggtag tcgggacaag
     1981 gcgtgcttct gaggcgacga gtcaggaagg gacggagcga cgtcgtggaa tcttagagga
     2041 tgatgatctt gcgccacctt ggcatcgggt tgcagtcccg ccgagggcag gagtgcgtcc
     2101 atctggtcag acgacaagat cggaggccct ccctccggca tggccggact ggagccaccc
     2161 ctgctccaga tccgacgtgc ccacccgccg gttggttctg catgccatgt aatgtcccat
     2221 tggcaccagt cgtcatcgtt ggcaatgcag gagcattcgg tcaaggtcgc cggaggcagg
     2281 ccgtgcaccc gtgctggaac ggcggccatg atgccctgcg ccgcctgaca catgacgaag
     2341 gtgcagcgac ggcgataggg tccgaactgg cgcagcgtcc gttcagaaaa ccgcatcgcc
     2401 agcgtggccg agcggttcgt gacgccgacg acacggcatt caatggagcc ggacgcaaac
     2461 ttgtttccga agtagggaaa catcccgtag atctgtgaca acgagaaggg cctggccaac
     2521 acctgcatga tcggcgacgc cttttctctc cccccgcggt agacgaactg gtcatcgccc
     2581 gaaatctgct cgcagaattc gtagagatag gcggtgaatt cgtaggagta gctgttccag
     2641 gcattcttca agaatgccgg tgtcacgtga tacacggggt tcttgatccg cccattcagc
     2701 gaggcacaca ggtcctgaac agcctgcagg cctgccgctt cgccgtggcg gcgtgttatg
     2761 aagctttcca ggtagaggac ggtcgcccgg atgctcatcc ctgtcaaatc acggatcttc
     2821 ttgccctgtt cgtccacccc gaacgggcga aattccatgt attctcgctc gagaatggtg
     2881 cgatatttcg gatacaaaac tattgtggtt gagcccggac gaagatcttg cgccgcacga
     2941 tcgtgcatgc ctgggactcc gtatggaggc gggagaggtg tagatgatgg cgccctgaac
     3001 cgcgccaccg atattcgggc ccgcctattg cctgattgac cgcttcaacg tcttttccac
     3061 gctggcgacc ttcgagacca ggcggcccga atggcccttt cgataatcca ccttgatgct
     3121 gcaggaaatg cgattgcaat ctttcttcat gcgttcgtag cacttccgga cgacggcgaa
     3181 gacctcgtcc cactcgcctt cgaggaccgt ccccatggga ttcagtcgat aggccacgcc
     3241 actcttgtca atgatatcca gcgagcgcga gacatacttg ctgacgcttt cgcctttgcc
     3301 tagcggggac atactgaatt ccagcaagac cattacgtcc tctcttctct tcgtgttatt
     3361 caggtgagcg gttaagacgt tcccttcccg cccgcttggg cagtcgctcg tctaacttct
     3421 tacccaccca ccctgcccgt gccgagacgc gggcttgtcc catgctcacc cacccaccct
     3481 agacgatcat ttgttacccg acccgcccac cgattgcttg gcaatcgttt acccggtacg
     3541 cgccttcgtc ttgtcccgct cttcgagcca gactttcgga tactgcacct tgccgtgaag
     3601 ccggaacccg tccaacgctt cgcgcaacat cgtgcggcgc ttctccacat caggctcatt
     3661 gctcttcgct tgttcgcgca actcgccgag ccactcgaca aaggcgacaa attccgcatc
     3721 cagtatgcgc tcaagatctt ccttcatgaa tcccgacagc gccggtgcca tcccgctggt
     3781 gctgatggcg acacgcgcat ggccggcttc gacaacggca ggcatattga ccgtggacgc
     3841 ctcgggaaaa tcgacggacc acaacaagaa cttccgctca cgcgccttgg caaacaacga
     3901 gcgtgcgaaa tcgcggtcgc cgcgcagcat gttgaggacg agcaccgcgc tttcgagatc
     3961 cgtttcgcga aaatgccgcc cccgatggat gatcttggcc gatgccgcca actgtttgag
     4021 ttgctcgtgc agcgtgggcg caatcacgat caccttggcg ccggcatcca acaagcgttg
     4081 cgttttttct gcggcttctt cgtcgcctcc caacacgagg accacgtagc ccttgacgtc
     4141 gagcgtgaga gggaacccag gattcgcggc catggtcatt cctccaattc caaaaggatg
     4201 gccatcatac aacagagcct tttccagcgt aaaccagtcg ctcccttttt ttattggagg
     4261 ccggtataat gcaccgcttt ttacaaccat tgcctgagag gatcgtgaat ggcagcatat
     4321 aaattcggcg ctgtgggaat ggccggcggc atcatcgcac tcgcggccgt cgccgcctgg
     4381 cttggcctcg cccacacgtg cgatccgcaa ggcggagagg cggtcatcgc gggacaggtg
     4441 gtgatctccc cgggactggc agatcacgtg cgtccgaccg atgtcctgtt cgtgatcgtc
     4501 aagagaccgg gcggcacacc aagaccggtg gccgcgaaac ggatcgagag ccccaaattt
     4561 ccagtaccct ttgaaatcac caatgccgac gtcatggtgc aaggctcgga actgcgaggc
     4621 atggtggacg tcatggcgcg attggatcgc gacgggcaag cagggccggc tcaaccgggc
     4681 gacatcgaag ggcgttacgc caagaatccg acgctccccg gcgggcggga tttggaaatt
     4741 atcttagata cggtgaaaca gtgagaagtg ctggaatcgg agtgcagtaa aacttgaggg
     4801 atgttcaaga agccggccgg aagaagtatg cacaccaggc gtgatggaca gcgcgataga
     4861 aggtgattac gggccagagt cagcgtcgag ctccatattc caatacaaat aatcccgcca
     4921 actctcaggt gtgttccgta ttccaatggt aatcgtgacg atcggactcc agcgcggctt
     4981 caccggccgt ttcatcagcc gcatcccggc ttcatccggt gtcctgccgc tcttgcgatt
     5041 gttgcagcgc cagcaggccg tcacgatatt ctcccacgtc ttccgtccgc ccttggcaat
     5101 cggcaccaca tgatcgaagg tcagttcctc ggtgcggaac ttggtgcggc agtactggca
     5161 gacataccca tcgcgggtga agatgttgat acgagaaaat ttcacggccc ggtggctgtc
     5221 cttcagtcgg accagcttga gcagccgcat gaccgcgggc agcttgaagg aaatggagac
     5281 gccgtgtacc tcgcggtcgt agacttcgag gacttctact ttaccttgcc agagaagagc
     5341 gatggctttt tgccaatgaa cgacacgcag gggttcatac gtagaattga gcaggagggt
     5401 catttccatt caacaacctc attcccaacc gaggctcctg ttcgatgaag ggccttgtat
     5461 cggtgtgggg tacgctcgcg tatgtctatg ccataagatt gggccgaaat caagcgcggg
     5521 ggccattctg cgggatcgaa ggcaaggaga gtacacccga ttgtgtgagg cagcatgtct
     5581 aagtttatca cggtcgcgaa gacgcaggac gttccgccgg gaaccggacg aaccgtcgag
     5641 gttcagggag tgtggattgc gctcttcaat gtggacgggt cgttttttgc ggtcgacaat
     5701 acgtgcccgc acgcgggagg accgatcggt gaaggctcgc tctctggcga cgtcgtgacc
     5761 tgcccatggc acggctggca gttcaatgtc cgcacaggcg agcggcccgg gaatcccaac
     5821 ttcaccgtcg cctgctgtcc ggtccgcgtt gagggcgagc agattcaaat tgcgctgccg
     5881 ccggatttca agtaataccc gtcaagcggg gccgagaata ttcggcttcg gcgccggcat
     5941 ggccggctct tcattctctg cgggcttcgg cgtgatccca tcggccggca acgacgccgc
     6001 attcactctt ttcagcgaca gattcgcatg ccagatgaat tctcgttcga accattgccc
     6061 gcccacaccc ttgtcacgca gctgcacacg cacttcatag agatcgccgt ccacttgctt
     6121 cacgcgccac tcgccgagcc gcgcgtttaa tccccggtct ttcatggaac ggacgtgttc
     6181 ggtcatcgcc tgcaaaatgg tgggatagcc gagcgcccgg tgagtctgga ccatcgccaa
     6241 cgcatccgcg cttcgccggt cgatcatcgg attgatcgta ggcggcttgg tccagatata
     6301 gtacacgacg cccaatagga tcacgccgag taccacataa tggagaattt tcaccatact
     6361 caaacggcct ttcatctctc agaaagcgag ccgcatctta ggaagtggtc tcacgccaag
     6421 tcaatcagac gaaatgacgc cccgtccgcg aacgagatcg atctcaaagc agttgttcaa
     6481 aatgggtcgt cggcaaggct gacgcccctt ttcaacaatt gccagagctt gtgccagtga
     6541 aagtgggtat gctagaagaa taccccctaa cacattcagc aatggtatga ggccatacag
     6601 gagttatgaa gttctttctc tacgccgata atctccatcc gactcaactc aagcgcaggg
     6661 ctccggaaca tcagtttcag atgctggcca ccctccccga ccatacgatc cagttctgcc
     6721 gctggtcgtc ccagtggcgg tgcgggctcg ccagcgtcgt accgtccccc ggcgagaaag
     6781 tatggggcgc aattttcgag ttgacggacg aagacatcaa actgctcgat caattcgaag
     6841 acgacgtgcc ccaggaagcc tttcgtcatg tccaggtgac ggtactcagt gaaaccggag
     6901 aaaagatcct ggtgactact catgcggcga cgccgatcgg aaaatttaag cccaaggaac
     6961 actatcttga ttgggtcatc aagggcttga agcattggaa atttccggac gaggtggtcg
     7021 agcagtggca agcctatcgg ccccattgat cggcgaatcc gatacgattg agttcagact
     7081 ggagaacagc gcggtacaca tttagcctga ggagcatatg cccaaaccaa aatatcacat
     7141 cgtcgtttgc acgaacgcca gaccaccggg acatcccaag ccctcatgcg gcagcgcagg
     7201 ctccgcgcaa ctgctgatgg cgttcaatat ggggctcatg cagcgcggtg tgatgccggg
     7261 cgaagtcctc gtcagcggct cgtcctgcct gggaccctgc gaacaaggac ccacggtcgt
     7321 cgtctatccc gagggcac
//
LOCUS       DASSCQ010000018        11820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_669368, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000018 DASSCQ010000000
VERSION     DASSCQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669368"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFH05_01155"
     CDS             <1..578
                     /locus_tag="VFH05_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6676853.1"
                     /translation="CWIVIHPGATALSRRYPLASFAAVAGQLARRHACQIVWTGSQAE
                     AGMIDEAQQAMQEPSVSLAGRLSVEELASVIAKAPLVISNNTSAVHLASAAGTPVVDL
                     YALTNPQHTPWRVPNRVLFEDVPCRFCYKSVCPEGHHRCLQGVSPDTVVEAALELLND
                     TRATERVSCIVPQRDNLSASGQFASSCRRPH"
     gene            complement(721..969)
                     /locus_tag="VFH05_01160"
     CDS             complement(721..969)
                     /locus_tag="VFH05_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676854.1"
                     /translation="MLLTHARAPIADIGAGSTQQLGRRRKPAHPADGQCAEIGAIPAK
                     TNAELSKLSVLAALHSDHIIGAAIADLSAGRTGINTVL"
     gene            1083..1559
                     /locus_tag="VFH05_01165"
     CDS             1083..1559
                     /locus_tag="VFH05_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6676855.1"
                     /translation="MLRIKDVMTPQAEVISPDATAEDAAAVMKTLDIGVLPVSDEQGL
                     VGILTDRDLVVRVMAATRDPKAMLVGEAMTPSVVYCFEDDDIEQAATVMAGQQIRRLP
                     VLDKNRKLVGIVSMGDIAVHGRDPQLTGKVLEDVSQPATPNRSDNANREGGFGQAA"
     gene            1846..2139
                     /locus_tag="VFH05_01170"
     CDS             1846..2139
                     /locus_tag="VFH05_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676856.1"
                     /translation="MQRLTYWESVSIGWRILWQAIGGFVLALFLSSMVVLGLLPELTR
                     TGPSYWALVVPLVIAFVFSLFVMLPLVVRGLINASFGTFRLVVTRKEPCHSTI"
     gene            2155..2529
                     /locus_tag="VFH05_01175"
     CDS             2155..2529
                     /locus_tag="VFH05_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676857.1"
                     /translation="MTRPTTHTIVTSALMALLTAGTLTHPVSTPAAEPEIKEGTLHLV
                     SGTLLRLDLLNGRGLLRTDLGRPIYFEVPKAYLFENVIVGARIALRLDDEGRAVRVMD
                     TAIADVVITPDQPLAATAPEDP"
     gene            complement(2552..3250)
                     /locus_tag="VFH05_01180"
     CDS             complement(2552..3250)
                     /locus_tag="VFH05_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6676858.1"
                     /translation="MRLTFATYNIHRCVGRDGRYDPDRIRDVIRELDADVIAVQEIDA
                     PDRHGLELAHWLGEESKLQVIAGPTLLERKGHHGNAVFTRCQRGDIRLLDFSVPMYEP
                     RGAIDLDLSCGGVTVQVIATHLGLRPWERRIQVERLLQRVGDNCVLMGDFNEWWLWGP
                     PLRAIKAIFGHSPALPTFPSFRPVLALDRIWIRPTGRVKSLTVHRTRLSAKASDHLPL
                     KAVVEWDAEENADR"
     gene            complement(3253..5436)
                     /locus_tag="VFH05_01185"
     CDS             complement(3253..5436)
                     /locus_tag="VFH05_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6676859.1"
                     /translation="MNTARKLQKPPESQRHHTGSSRLSLFDVGRNCWRIARADRASFL
                     VDGEAYFRAFREAAIQARHHIIILGWDFDSRISMLIDREPDGFSDRLGEFLHDLLIRR
                     RDLHIYVLTWDFHMIYWKEREWWLPSKLVAHRRLHFYKDGAHPVGASHHQKIVVIDDS
                     LAFVGGMDFAQCRWDTNRHAAHHPKRLLLQNGGPCRPFHDVQLMVNGPVAAALGELAR
                     DRWRFATGRFIPPVKTARVEDLWPQGAEPDLSAVPVAIARTAPPFAERPPIGEVEALF
                     LDSLNRAQRYIYIETQYLTSHSIADCLAARLRDPKGPEIVMVLHPNSDGWLEQHTMDV
                     LRGRVLQRLRAADKFHRLNLNYPKIPDLHGQCISMHSKVCIIDDEIVRIGSANLSNRS
                     MGFDTECDLAIEAAGDPTVRRAIARFRHTLLGEHLGISPDEVQRELDKDGSLIGAVER
                     LRGGARSLGHFDGTVSADVNEVVPDEAFIDPSRPYDAQFFPVERRKPAVRQIVAGTAG
                     LFLLVLLAGLWHWTPLRDHIEVSSLVNYVEDFASGPLALPITITGFLIGGLLVMPVLI
                     LIAVTILAFGPWWGFWYALIGMTASAMLTFWIGRLLGRRVVDYLPGSRVHRISRALAA
                     KGILTVVALRILPVAPFSILNAMAGASHIRARDFFMGTVLGEMPGLVGLALFLDQVTE
                     TIRHPGWSSILILVGIACLMVIVAWGLSRWLSSRTTEGDYEKSPA"
     gene            6017..6880
                     /locus_tag="VFH05_01190"
     CDS             6017..6880
                     /locus_tag="VFH05_01190"
                     /inference="COORDINATES: protein motif:HMM:NF005214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6676860.1"
                     /translation="MADTTKQQRPPQHQSRQPGLETAMTPKPESERPEQPGCGKLRDK
                     VALITGGDSGIGRAVAIAYAKEGADIAIVYLNEHVDAEETKRMVEGEGRRCLVIAGDV
                     GDEHFCRQAVQQTVREFGRLDILVNNAAEQHPQDSLEHITAEQLERTFRTNIFSYFYM
                     GKAALPHLKEGSAIINTTSVTAYKGSAQLLDYASTKGAIVAFTRSLSQALAEKKIRVN
                     AVAPGPIWTPLIPSTFPADKVASFGSDVPLKRAGQPEEVAPSYVFLASQDSSYTTGQV
                     LHPNGGTVVNG"
     gene            6925..7542
                     /locus_tag="VFH05_01195"
     CDS             6925..7542
                     /locus_tag="VFH05_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6676861.1"
                     /translation="MAKRTPKSKSSQSLDAVQLLKADHKQVRKLFEEFHGASDDDKGS
                     IARRLFTELEIHSKLEEELLYPAVQAKLESLDYETEGNGLDVSDDGERDQEGEDALDD
                     IDGAELEVLEDETDEEAESEIITAAYDSHGTVRELIQQLQSLDPKSTDYREMFTELED
                     AVIEHVAEEEDAILPLAAAQLDVLALGMTMQQRKDDLTSQSSLAA"
     gene            8024..8431
                     /locus_tag="VFH05_01200"
     CDS             8024..8431
                     /locus_tag="VFH05_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET6676862.1"
                     /translation="MDNQTNERRPEVLRTLLIGRRSTLQREIDHLIAQHRDNHTHFRD
                     NSVLDVEDMSLRDATGAQQIALLEARTQERIQLDEALRRLDEGTYGICEDCEAPISPA
                     RLRALPFARRCVGCQEQYELFQNIVRREDREEI"
     gene            8442..8987
                     /locus_tag="VFH05_01205"
     CDS             8442..8987
                     /locus_tag="VFH05_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6676863.1"
                     /translation="MREAKSRNDDMEDRDMEEEEETSGDLFEMLKTDHRHVQDLFTRF
                     EDADKRSRSTIAEEALVALEVHAALEEELVYPAIAEVVEEEELVNEAKEEHHVAKLLI
                     KELHKMDAGDEEFATKFKVLGEIVGHHIEEEESEMFPQAQEGGFEPETIAQQVVKRRE
                     KLMQKFQGGKKSSASKRKKAA"
     gene            9123..9596
                     /locus_tag="VFH05_01210"
     CDS             9123..9596
                     /locus_tag="VFH05_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676864.1"
                     /translation="MHVGDMLLWGLLATGLLTTVLRAGQALHLTRMDTALVLGTMVTG
                     NRDRAKVIGFVIHFFNGWWIATLYILFFHAFGMATWWLGALMGIAHTAFILVAVLPSL
                     PGVHPRMASDVTGPSPTRMLEPPGFLALNYGFRTPLAVLLAHIAYGTVLGAFYQV"
     gene            complement(9635..11425)
                     /locus_tag="VFH05_01215"
     CDS             complement(9635..11425)
                     /locus_tag="VFH05_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET6676865.1"
                     /translation="MTAYPPIHDYALIGDCRTSALISSSGSIDWMCLPRFDSPSCFNR
                     LLDWEHGGYCAILPTAPFRTRRFYHLNTAIVTTEFRTFDGLAQLTDLMPVTADGELAA
                     VPHPSRQVLRRLEVLEGTIEFSLMVKPRPNDGRTVPCFESRGRAGYCADFGGSVLFVA
                     VDSPMEIRNGDLTGRVVMDRGESLTLWMAYEEEAPGVYPRLSDAKTAIADTEAYWSGW
                     AAHCQYVGPFKDQALRSAITLKLLSYAPSGAIVAAPTTSLPERIGGELNWDYRYCWLR
                     DASFTAHAFFRLGFHAEALAFMQWITHATTLTYPELQVMYTLHGEASIPERTWPFFEG
                     YRGSQPVRFGNAASQQHQLDVYGEVLDALSLSVEAGYPVDRDTRRWIVEVGNLLCASW
                     PYPDHGIWEVRGEPRHYVHSKVMCWTALDRIERLTKKLGLRASTGPWVQARDALARTV
                     LKTGYSKTRRSFVQVLGGTRLDAAALTFGLVGFIDGRDPLMTSTILAIQRWLTRGPLV
                     YRYVAEQGTGREEGAFLPCSFWLVEALALAGRMGEAEDFAYRLHAHGNDLGLFPEEIR
                     HQDGAFLGNFPLALTHVTHLSALLRIAGKE"
     gene            complement(11516..>11820)
                     /locus_tag="VFH05_01220"
     CDS             complement(11516..>11820)
                     /locus_tag="VFH05_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676866.1"
                     /translation="VMLLLGGCAGGGQEFGGAGTASSQQEAASQRTDYRTQANALREM
                     ADRRQLEADLLARELGATDPSVEKKRRLAEELRAAAEDADNKARSLSRNVPHGMVQ"
BASE COUNT         2617 a         3479 c         3435 g         2289 t
ORIGIN      
        1 ggtgctggat cgttattcat cccggtgcga ctgccctgtc tcgtcggtat cctctcgcct
       61 cattcgcggc cgtcgccgga caactggcac ggcgtcacgc atgtcaaatc gtgtggaccg
      121 ggtcacaagc ggaagcaggc atgatcgacg aggcgcaaca agccatgcag gaaccatccg
      181 tgtctctggc cggacggctg agcgtcgaag aactggcatc ggtaattgcc aaagcgccgc
      241 tcgtgatctc caataatacc agcgccgtcc atttggcatc agcagccgga acaccggtgg
      301 tcgacctcta tgccctcacc aacccacagc atacgccgtg gcgggtgccc aatcgcgtcc
      361 tcttcgaaga cgttccctgc aggttttgct ataagagcgt ctgtccggaa ggtcatcacc
      421 gatgcttgca aggcgtctcc cccgacaccg tcgtcgaggc cgcacttgag cttctgaacg
      481 acacccgagc gacagagcga gtctcctgca ttgttccgca aagagacaac ttgtctgcgt
      541 caggacaatt tgcgagctca tgtcggcgac ctcattaggg tattaagaga ccgctcgtat
      601 tcgtttcgag tttttagcca ctcgtagctc ggctggtctg tcttttgcgt cctcagcgca
      661 ttttattctc tgcccgaaag gagcggtatg caccatcgtg atcacgcccc acatccgcac
      721 ctacaacact gtattgatac ctgtgcggcc tgcgctcaaa tctgcgatcg ctgcgccgat
      781 gatatgatcg gaatggaggg cggcgaggac cgagagcttc gagagctctg cattcgtctt
      841 tgccgggatt gcgccgatct ctgcgcattg tccgtccgct ggatgagccg gctttcgcct
      901 tcgaccgagt tgctgtgtcg aacctgcgcc gatatctgcg atcggtgcgc gagcgtgtgt
      961 gagcaacacg cctcgcatca tccactttgc ggcgattgtg cggttgaatg ccgccgctgc
     1021 gcggcagcgt gccgggacat ggcgacagcc gcagcctaac tgcccgatga caaggaggag
     1081 ccatgcttcg aatcaaggac gtgatgacgc ctcaggccga agtgatttct cccgatgcca
     1141 ctgctgagga cgcagcggca gtcatgaaga cactggacat aggggtcctc ccagtatccg
     1201 acgagcaagg gctggtggga atattaacgg atcgagacct cgtcgtgcgc gtgatggccg
     1261 cgacccgcga tccgaaggca atgctcgtcg gcgaagcaat gaccccgtcg gtagtctatt
     1321 gtttcgaaga tgacgatatc gagcaggccg ccacggtgat ggcaggacaa cagattcggc
     1381 gtttgcccgt actcgacaag aaccggaaat tggtcgggat cgtgtcgatg ggcgatattg
     1441 cggttcacgg gcgcgatccg cagctcaccg gaaaggtgtt ggaggacgtg tcgcaaccgg
     1501 caacaccaaa tcggagcgac aacgcgaacc gcgagggcgg attcggtcaa gcggcatgaa
     1561 ctgagatgcc tatgctgccg agttcacggt atgtcctggc tggagcattg ggtttcacct
     1621 tggcaatcgg gtggggtcgc ttggtcctgg ccgttgagcc cccaagtccg actcctggcc
     1681 ccacgtttcc caatccccag cctactccgc caactccggc tcctgtgccc catccgacgc
     1741 cgccaacgcc cggtcccact cagcctacac catcgccggg gccgacccag ccctaagctg
     1801 aggaaccaca ctgcatgtta aacataacct attccacgaa tcattatgca aaggctcacg
     1861 tattgggaat cagtatccat cggatggagg attctctggc aagccattgg tggtttcgtg
     1921 ctggcgctgt ttctgagtag catggtggtg ctcgggctgc tgcccgagct gacacgcacg
     1981 gggccgtcgt actgggcgct tgtcgttccc ctggtgatcg cctttgtgtt ttcgctgttc
     2041 gtaatgttgc cgttggtcgt tcgcggcctg atcaacgctt cctttgggac cttcaggctt
     2101 gttgtcacac gcaaggagcc ctgccactcc accatctagg caggaggagg cactatgacc
     2161 cgtcccacaa ctcacacaat agtcacgtcc gcgctgatgg ccctgttgac cgccgggaca
     2221 ctgacacatc ctgtgtccac accggcagcc gaaccggaga tcaaggaagg cacgctgcat
     2281 cttgtttccg gcaccttgtt gaggctcgat ctgttaaatg ggcgaggact actcaggaca
     2341 gacctgggcc gcccgatcta tttcgaggtg cccaaggcat atttgttcga gaacgtgatc
     2401 gtcggcgctc gcatcgctct gcgactcgac gacgaaggcc gtgcggtcag ggtcatggac
     2461 accgccattg ccgatgtcgt catcacaccc gatcaacccc tggcggcaac cgcgccagaa
     2521 gacccttgac gatcgggaag ccatctcatc gttaccggtc ggcgttttcc tccgcgtccc
     2581 attcgacgac cgccttgagc ggtagatggt ccgaggcctt ggccgatagc cgtgtccggt
     2641 gaacggtaag ggacttcact cgtcccgtag gacggatcca gatccggtca agcgcgagga
     2701 ccggccggaa ggacggaaag gttggcaagg caggtgaatg accgaagatg gctttgatcg
     2761 cccgcaatgg aggcccccat aaccaccatt cgttgaaatc ccccatcagc acgcaattgt
     2821 cccccacccg ctgaagcagc cgctcgacct gaatacggcg ttcccatggc cgcaagccga
     2881 gatgagtggc aatgacttgc accgtcacgc ctccgcacga aaggtccagg tcgatggcac
     2941 cacggggttc gtacatcggc acgctaaagt ccagcaagcg aatgtccccc cgctggcagc
     3001 gcgtgaatac cgcattcccg tggtgcccct tacgttccag gagcgtaggc ccagcgatca
     3061 cttgcagttt ggactcttcg cccaaccaat gggcaagttc aaggccgtgc cggtccgggg
     3121 cgtcgatttc ttgtacggca atgacatctg catcgagttc gcggatgaca tcgcgaatgc
     3181 ggtcagggtc ataccgtcca tcccggccga cacagcgatg aatgttgtag gtggcgaagg
     3241 tgaggcgcat cgttatgccg gggacttttc gtagtctcct tccgtcgtcc ggctcgacaa
     3301 ccagcggctg agcccccacg cgacaatgac cataagacag gcaattccca ccaagatgag
     3361 gatgctgctc caccccgggt gacgaatggt ctccgtcact tgatcaagga agagcgcgag
     3421 gcctaccaga cccggcattt caccaagcac ggttcccata aaaaaatccc ttgccctgat
     3481 atgcgaagcg ccggccatcg cattgagaat ggaaaacggt gcgacaggga gaatgcgcag
     3541 cgcgacaacg gtcaggatac ccttcgccgc cagtgctcgg ctgatgcgat gcacacggga
     3601 ccctgggagg taatccacaa cccgccgccc gagcaaccgg ccgatccaaa acgtcaacat
     3661 ggcgctcgcc gtcatgccga tcaatgcata ccagaatccc caccatggtc cgaacgccag
     3721 aatggtcacc gcaatcagaa tcaacaccgg catgacgaga agtccgccga tcaagaatcc
     3781 cgtgatcgtg atgggcaagg ccaatggccc gctggcaaag tcttccacat aattcacgag
     3841 ggaggagact tcgatgtggt cgcgcaaggg cgtccagtgc cacaagcccg ccagcaagac
     3901 cagcaggaat aatccggccg tgcctgcgac gatctgccga acagccggct tgcgccgttc
     3961 cacgggaaaa aattgcgcat cgtagggccg gctaggatcg ataaacgctt cgtcgggcac
     4021 gacctcattc acgtccgccg acacggtccc gtcaaaatgt ccgagtgacc gcgctccacc
     4081 gcgcaaccgt tcgaccgctc cgatcaacga accatccttg tcgagttctc gttgaacttc
     4141 atccgggcta atgccgagat gctcccccag taaggtatgc cggaatctcg cgatggcacg
     4201 ccgaactgtc ggatccccgg ccgcttcaat cgccagatcg cattcggtat cgaaccccat
     4261 ggagcggtta ctaagattgg ccgagccgat acggacaatc tcatcgtcga tgatacacac
     4321 cttgctgtgc atgctgatgc attgtccgtg gagatcggga attttgggat aattgagatt
     4381 caagcggtga aatttgtccg ccgcccgaag tcgctgtagt acccttcctc gcaggacgtc
     4441 cattgtgtgt tgttcaagcc agccgtcact attcggatgc aacaccatga cgatttccgg
     4501 ccctttcggg tctcgaagac gcgccgcaag gcagtcagct atcgagtgtg acgtcagata
     4561 ctgggtctca atgtagatat accgctgtgc cctgttcaga ctgtctaaaa acagcgcttc
     4621 aacttccccg atcggcggac gctccgcaaa gggcggcgcc gttcgcgcaa tggcgaccgg
     4681 aacggcggac aaatcgggtt ccgcgccctg aggccagaga tcctccacac gcgccgtctt
     4741 caccggcgga ataaagcggc cggtggcgaa gcgccaacga tcacgagcca gctctccgag
     4801 cgcggccgcg acggggccat tgaccatgag ctgaacatcg tgaaacggcc ggcatggccc
     4861 cccgttttgc aagagcagac gcttgggatg atgggcggcg tgacggttgg tatcccaacg
     4921 gcattgcgcg aaatccattc cgcccacaaa ggccaacgaa tcatcgatga cgacgatctt
     4981 ttgatgatgg gacgcgccca ccggatgcgc tccgtcttta taaaaatgca agcgtcggtg
     5041 tgctaccagt ttggaaggaa gccaccactc ccgctccttc cagtaaatca tgtgaaaatc
     5101 ccaggtcagc acatagatat ggagatccct cctccgaatc agcagatcat gcaaaaactc
     5161 tccaagacgg tcagagaaac cgtccggttc acgatcgatc agcatgctga tgcggctgtc
     5221 gaaatcccag cccaggatga tgatgtgatg gcgtgcctgg atggcggctt cgcggaaggc
     5281 ccgaaaatag gcctcgccat cgaccaagaa tgaggcacga tcagcgcggg cgatcctcca
     5341 acagttcctg ccgacatcaa aaagagacaa tcgtgaggaa ccggtgtgat gacgctgaga
     5401 ttccggaggc ttctgcaatt ttctcgcggt attcatatgg cggtcagtgc agaactgcaa
     5461 atcggatgcc tttgctcggg tgaaacgggt aaacggcagc accttcaagc gagaggaccg
     5521 cgccacattg tgaacgacgg gaccagctgt cccatgtcag acactttgtc cccctgagcg
     5581 cagctagcat gctgaagtgc tccagcctcg cgagacaata ccagcccgca tgcacagcgc
     5641 atctgggtct acctcttgca cccgtcttca gtacctctga ggagagaggg aaatgttttt
     5701 gcgccgggag ccgttactcg tggtgtgcag cctggtggtt gtagtggggt gggcctcgtc
     5761 aagcgcgatc gcccaggcgg cttctgcgtc atcacctttg gaacaatcca aaaataaatt
     5821 taggccaggc cgaggcccag gaaagaccgg tcaaggtggg accggcagcg gagcgagccc
     5881 ggggccgggc agtggctctg gaatgggcag tgcaggcagc ggtcattcgg gagccggcgg
     5941 gcagagtcac agcggcggga gagcgacaac caacaacaac ggatcaaccg ggaccatagg
     6001 gagataacca gaggacatgg cggacacaac aaaacagcag cgcccccccc agcaccaatc
     6061 gcgtcagcct gggctcgaaa cagcgatgac accgaagccg gaaagcgaaa gacccgagca
     6121 acccggatgc ggaaaacttc gcgataaagt ggcgctcatc accggcggcg atagcggaat
     6181 tggccgtgca gtggcgattg cctatgcgaa agagggcgcc gatatcgcga ttgtctattt
     6241 gaacgagcac gtggatgccg aggagaccaa gcgcatggtt gaaggtgagg gccgtcgctg
     6301 tctcgtgatt gcgggtgatg tcggagacga acacttctgc cggcaggccg tccaacaaac
     6361 agtccgtgaa tttggcaggc tcgacatact ggtgaacaat gcggcggaac aacaccccca
     6421 ggacagctta gagcacatca ccgcggaaca gttggagcgc acgtttcgca cgaacatctt
     6481 ctcgtacttt tacatgggga aggcggcgct gccacatctc aaagaaggga gtgccatcat
     6541 caatacgacg tcggtcaccg cctataaagg cagcgcgcaa ctgctcgatt atgcttcgac
     6601 caagggtgcg atcgtggcgt ttacccggtc attatcgcaa gccctggctg agaaaaagat
     6661 tcgcgtcaac gccgtggcac cgggccctat ttggactcct ctcattcctt ccaccttccc
     6721 ggctgacaag gtggcatcat tcggttccga tgtccccctc aaacgcgctg gccaaccgga
     6781 ggaagtcgcc ccaagctatg tgtttcttgc atctcaggat tcttcttaca cgaccggtca
     6841 agtgctgcat cccaatggtg ggacggtcgt gaatggctaa cgccccacgt gcaggggctt
     6901 cacgtgaacg gcaaggagga agctatggca aagcggacgc cgaagtccaa aagttctcag
     6961 tcgcttgatg cagtgcaatt gctcaaggcg gatcataaac aggtacgaaa gctctttgag
     7021 gagtttcatg gagcttcgga cgacgacaaa gggtcgattg cccggcggct gttcactgaa
     7081 ctcgagattc attcgaagct cgaagaagaa ctcctgtacc ccgcagtcca ggccaagctc
     7141 gaatcgctcg actatgagac cgaaggcaat ggcctggatg tttcagatga cggcgagagg
     7201 gatcaagaag gggaagacgc actggatgac atcgacggag ccgagctcga ggtgttggag
     7261 gatgaaacag atgaagaagc ggagtcggaa attatcaccg cggcctatga cagtcatgga
     7321 acggtgagag agctgattca gcagctgcaa tctctggatc ctaagagcac tgactaccga
     7381 gagatgttca ctgagttgga agatgctgtg atcgagcacg ttgcggaaga ggaggacgcg
     7441 atcctgccgc tcgccgctgc acagctagac gtgctggcgc ttggcatgac gatgcagcaa
     7501 cggaaggacg acctgacgtc gcaatcctcc ctcgcggctt aaacgacggt cgagtacgac
     7561 tgcatgccgc ggagagatcg ttccctgtga agaaaggcgt tgtcttccgt cgacgacgaa
     7621 accgacggga catgaacgat ccgggcgcgc ccccgtttcg gatcgctcgt cattgagcct
     7681 gagagtcgcc cactctcgat ttcgtgacac acccgcgatt cgtcgaatac ggcgaaagac
     7741 ggcacaggca gaggagatct cttctcctct gcctacctgt cagccagttt ccgacatatg
     7801 cagttcgcac ctcttcgccg cttgcccact caccgacgag ttgtccgctg cggagacaag
     7861 tattccgcct cacacagaca tcatgtgtct gcgccaggcg gagcgcgcaa ggaagtccct
     7921 gctaacccgc ccaccggcag cgccgacgga agcacagagc actggccctg cttttgcaga
     7981 accggatgcg aagctgctta tcattattca cgaggagtct gtcatggata atcagaccaa
     8041 cgagaggcgt ccggaagtgc tgcgcacctt gctgatcgga cggcgttcga cgctccagcg
     8101 agaaatcgat cacctgatcg ctcagcaccg tgacaatcac acgcactttc gcgacaactc
     8161 tgtcctcgac gtcgaagaca tgtccttgcg cgatgccacc ggggctcaac agatcgcctt
     8221 gctggaggca cgcacgcagg aacggattca gttggacgag gccctgcgca ggctcgacga
     8281 agggacgtac gggatctgcg aggattgcga ggcccctatc tctccggcgc gtctacgagc
     8341 gctgcctttt gcgagacggt gcgtcggttg ccaggagcag tacgagctgt tccaaaacat
     8401 cgtacgccgc gaagaccgtg aagaaatcta aaggaggtac catgcgggaa gccaaatcaa
     8461 gaaacgacga tatggaagat cgggacatgg aggaggaaga ggaaacttcc ggtgatctct
     8521 ttgagatgtt gaagacagat catcgtcacg tgcaggacct ttttactcga ttcgaggacg
     8581 cagacaaacg aagccgctcc acgattgccg aggaagctct tgtagcgctc gaagtccacg
     8641 ccgcgttgga agaagagctt gtctatccgg cgattgcgga agtcgtcgag gaagaggaac
     8701 tcgtcaatga ggcgaaggaa gagcatcacg tggcgaaact gctcatcaaa gaattgcata
     8761 agatggacgc gggagacgag gaattcgcga ccaaattcaa agtgcttggc gagattgtcg
     8821 gtcatcatat cgaggaagaa gaaagcgaaa tgttccccca ggcccaggag ggtggtttcg
     8881 agccggagac catcgcacaa caggtggtga agcgaagaga aaagctgatg caaaaatttc
     8941 aagggggaaa gaaatcctcg gcctccaagc gaaaaaaggc cgcatagagc gaaccccggt
     9001 cgtcgggtct gtggtcttcg ggtgcctgag tatgatcggg ttaacccggt ccatgggccc
     9061 gacgcgaccg gctagggttc ggacacatcg acgagccgcg agaccggaag gaacaggcgg
     9121 tcatgcatgt cggtgacatg ttgctgtggg gactgctggc gacgggcctg ctcaccacgg
     9181 tccttcgggc cggtcaagcg ctccacctga cccgtatgga tacggccctg gtactcggga
     9241 ccatggtcac cggtaatcgt gaccgggcta aggtcatcgg cttcgtcatc catttcttca
     9301 acgggtggtg gatcgccaca ctctacatcc tcttttttca tgccttcggt atggctacct
     9361 ggtggcttgg ggcgttgatg ggtatcgcgc acacggcatt catcctggta gccgtcctgc
     9421 cttctctgcc aggagttcat ccgcgcatgg cttcggatgt gacaggcccg agtcctacga
     9481 gaatgctaga gcccccgggc ttcctggccc tgaattacgg cttccgcact cccctcgccg
     9541 tccttcttgc ccatatcgcc tatggcaccg tcctcggcgc gttttaccag gtgtaacaat
     9601 ggcagatgaa gcctccgcct atctgggtca cgcttcactc tttaccggca atgcgcagga
     9661 gtgcactgag atgggtcaca tgggttaagg caagagggaa gtttccgaga aacgcaccgt
     9721 cctgatggcg aatctcttct gggaacagcc ccagatcgtt gccatgcgca tgcagccggt
     9781 aggcgaagtc ttcggcttca cccatccgtc ccgcaagagc gagagcctcc accaaccaga
     9841 aactgcaagg cagaaatgcg ccctcttcgc gaccggttcc ctgctccgcc acataccgat
     9901 acaccaaagg cccccgtgtg agccagcgtt gaatcgctaa gatcgttgac gtcatcagcg
     9961 gatcgcgtcc atcaataaag ccgacaagtc cgaaggtcaa agcggcggca tccaagcgtg
    10021 tcccgcccaa gacctgcaca aaactccgcc gggttttcga gtagcctgtt ttcagcaccg
    10081 tgcgggccag tgcgtcgcgt gcctggaccc aggggcccgt agaggcacgc aggccgagtt
    10141 tcttcgtcag acgctcgatg cgatcgagtg cagtccaaca catgactttc gagtggacat
    10201 aatgccgggg ctctcctcgc acttcccaga ttccatgatc gggatacggc catgaagcac
    10261 agaggagatt gccgacttcc acaatccagc gtctggtatc ccgatccacg ggatagccgg
    10321 cttcaaccga cagggataag gcatcaagca cttcaccgta cacatcgagc tgatgttgtt
    10381 gggaggcggc attcccaaag cggaccggct gtgatcctcg ataaccttca aagaacggcc
    10441 aggtgcgttc cggaatcgag gcttcaccat ggagagtgta catgacttgc agttcgggat
    10501 aggtcagcgt ggttgcatgc gtgatccatt gcatgaacgc taaggcttcg gcgtgaaatc
    10561 ccaggcgaaa aaacgcgtgt gcggtgaatg aagcatcccg caaccagcag taccgatagt
    10621 cccagttcaa ttcgccgccg attcgctcgg gaagcgatgt ggtgggcgca gccacgatag
    10681 ctcccgacgg cgcatagctc aacagcttca gggtgatagc gcttctcaga gcttgatcct
    10741 tgaaaggacc gacgtactga caatgcgctg cccagccaga ccagtacgcc tctgtatccg
    10801 cgatggcggt cttcgcgtcg gagagccggg gatacacccc cggcgcctcc tcttcgtagg
    10861 ccatccacag ggtgagactt tctcctcggt ccatgaccac acgcccggtg aggtctccat
    10921 ttcggatctc catcggactg tccacagcca cgaagaggac cgaccctcca aaatcggcac
    10981 aatacccggc cctccctcga gattcgaagc aaggaactgt ccggccgtcg ttcggtctcg
    11041 gtttcaccat gagggaaaat tcgatcgtgc cttccaacac ctcaagacga cgcaggacct
    11101 ggcgcgatgg gtgcggaaca gctgccagct cgccatcggc agtcactggc atgagatcgg
    11161 ttaactgggc gaggccatcg aatgttcgaa attctgtggt aacaattgcc gtattcagat
    11221 gataaaagcg tctggtccga aaaggcgcgg taggcaggat ggcgcagtac ccgccatgtt
    11281 cccaatccag caggcggttg aagcaggatg gactgtcgaa gcggggaaga cacatccagt
    11341 caatagaccc gctgctggat atgagcgcac tcgtccggca gtcgccaatg agggcgtagt
    11401 cgtggatcgg gggataggcc gtcaaggtga ggcctcaggt atcacgaagt ctccgataag
    11461 gagaggcgcg gttacgtttc gatgggaaac ccgtcggccg gcacagtcgc acggtctact
    11521 gcaccattcc gtgaggcacg ttgcggctga gactgcgggc cttgttgtca gcatcctccg
    11581 cggccgctcg aagctcttcc gctaaccgac gcttcttctc gacactgggg tcggtggcac
    11641 cgagttcccg ggctaacagg tcggcctcga gctgccgccg gtcggccatt tcgcgcaacg
    11701 cgtttgcctg cgttcgataa tcggtgcgct gggacgcggc ttcctgctgg gaagatgccg
    11761 ttcctgcacc tccgaactct tgcccgccgc ctgcgcaacc gcctaacagg agcatcaccg
//
LOCUS       DASSCQ010000019        24518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1261619, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000019 DASSCQ010000000
VERSION     DASSCQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 24518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24518
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261619"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFH05_01225"
     CDS             complement(<1..289)
                     /locus_tag="VFH05_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6676867.1"
                     /translation="MFKVTPADRPEFLAGDHTRLREVFHPAKHPLKLGYSLAHGTLGP
                     GQRSKWHVLSSSEVYYFLTGHGRFMIDEQVTTVEAGTTLYVPPGSKQSLENI"
     gene            complement(351..1898)
                     /locus_tag="VFH05_01230"
     CDS             complement(351..1898)
                     /locus_tag="VFH05_01230"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET6676868.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKVMVAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIAQEVEGPTICSLARARPEDIDRAWEALKGAPKVRIHTFLST
                     SDIHLKHQFRMTREEAKKRAVEMVQRARGYVEDVEFSPMDASRSDPAYLHEVIEAVIE
                     AGAGTVNIPDTVGYAIPQEFGKLIKGIRERVANSGKAVISVHCHNDLGLAVANSLAAV
                     MEGAGQVECTINGIGERAGNTSLEEIAMGLRTRKDLYQADTRIHTEEISKTSRLVSKI
                     TGMVVQPNKAIVGANAFAHTSGIHQDGLLKDKTTYEIMRPESIGLVESKMVMGKLSGR
                     HAFRQRLEELGYKLTEEEVNHAFERFKRLADHKKEIYEEDLEVIVSEELAKMADRITL
                     KSFSVSSGTDQVPTATVELEIDGKSLSQRGTGDGPVDAVYRTIAAITQTKSTLLMYVV
                     KAITGGTDAQGEVSVRVQEDGRTVSGHGADTDIITASARASLNALNKLAYLATKQAQG
                     EQKVNLI"
     gene            complement(2392..3192)
                     /gene="pssA"
                     /locus_tag="VFH05_01235"
     CDS             complement(2392..3192)
                     /gene="pssA"
                     /locus_tag="VFH05_01235"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET6676869.1"
                     /translation="MKNTGLRQSFAKGTKRHKAAMHLIPNLFTTGNLFCGIFAVLSVF
                     NADYMAAAIAILVAMIFDVLDGKSARMTNSTSHFGLEYDSLSDLVSFGVAPGLLLYSW
                     ALSGQGTFGIAVMFAYVAMGAVRLARFNATTTMADGKYFTGLAIPAAAGVVASMVIFD
                     HYIVRVGAEVKPVAVLIVTLILSFLMVSTIKYRSFKDLKFRGRQHITYLVWGILALVL
                     VAAWPQVMLFVMFAGYALLGPVERMFVVLTKFAGKQPPSKREVVEQKW"
     gene            complement(3189..3839)
                     /locus_tag="VFH05_01240"
     CDS             complement(3189..3839)
                     /locus_tag="VFH05_01240"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HET6676870.1"
                     /translation="MTDRAAGVPFAKEGFPFIGAAAGITLIAWWLGWIPVAVVGAMLT
                     LFVSWFFRNPPRRVPQGPGLVVAPGDGVVLAVEEEFEPRYLKDRAVRISIFLNVFDVH
                     INRMPCEGIVENVQYQPGLFLVASKPHATVKNEQNAVMIKTVEGAKVLCVQVAGLIAR
                     RIVCWVNPKDRAVRGERYGLIRFGSRMDTYLPVGTAIRIAVGNRVTGGETILGELQ"
     gene            complement(3855..4871)
                     /gene="ilvC"
                     /locus_tag="VFH05_01245"
     CDS             complement(3855..4871)
                     /gene="ilvC"
                     /locus_tag="VFH05_01245"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET6676871.1"
                     /translation="MMKIYYDRDADLQQITNKKVAVIGFGSQGHAHALNMKESGVSVV
                     VGLREGSSWRKAEQSGLKVMPVADAVKASDVVMILAPDEAQAALYRQEVAPNLKAGSY
                     LAFGHGFNIHFGQIVAPPTINVFMVAPKGPGHLVRSEYTKGSGVPCLLAVHQDPSGTT
                     KQVGLAYASAIGGGRAGVIETNFREETETDLFGEQAVLCGGLTSLIQAGYETLVEAGY
                     SPEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRIVTEQTKQEMKK
                     ILGEIQQGQFAKEWVLENQANRPVYNALLAKGEAHPIEEVGAKLRAMMPWLKKDQLVD
                     KSKN"
     gene            complement(4930..5505)
                     /gene="ilvN"
                     /locus_tag="VFH05_01250"
     CDS             complement(4930..5505)
                     /gene="ilvN"
                     /locus_tag="VFH05_01250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET6676872.1"
                     /translation="MDGCRDPQRHAGTDDKTRTMEHIILVTVENKFGVLSRIAGLFSG
                     RGFNIESLSVAPTLDPSMSQMTIVTSGDERIVEQIVKQLNKLIDVIKVVDLNESDFVS
                     RETALIKVHTRQEDRAEALRIADIFRANVIDSTPATYTIEVTGDPKKIEAIINLLQPL
                     GIKELTRTGRVAVAREPLRSTTTQPKKVARE"
     gene            complement(5512..7287)
                     /gene="ilvB"
                     /locus_tag="VFH05_01255"
     CDS             complement(5512..7287)
                     /gene="ilvB"
                     /locus_tag="VFH05_01255"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET6676873.1"
                     /translation="MKLTGAEIFIECLKREGVKTLFALPGGVVLKIFDMLHQQKDIEV
                     VLTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLVCFSGQV
                     PTNLIGNDAFQEADNVGLSRPCTKYNFLVKDVNELASTIKEAFYIATTGRPGPVLVDI
                     PKDVSMDKAEFNYPNSVSIRGYNPTYEGNKWQVKQAAEAIMKSKKPILYVGGGVIFSG
                     ASKELIELAELTQIPVDMTLMGLGAFPGEHPLSLGMMGMHGTYQANMAVHYSDLVVAI
                     GARFDDRVTGKVSEFCPYAKIIHIDIDPTSIRKNIHVDIPIVGDCKAVLRELNQILRA
                     TVNGEQKELRKAWWDQIREWQSAHPLSYQQEADGPIKPQQVIKRLYELTKDLDPIVST
                     DVGQHQMWAAQYFKMAKPNRWLTSGGLGTMGFGFPAAMGAQAAFPGRLVLCIAGDGSV
                     QMNMQEMATAVVHKLPVKIIVLNNRFHGMVRQWQDLFYEGRYASSYLDTTPDFVKLAE
                     AYGAIGLRPSKVSDLDNVLQEAIAAKGPVIVDVPTHPYENVYPMIPAGGCNHEMILDD
                     PPELKLKQSGGAKVTPQDKDTILTA"
     gene            complement(7525..7948)
                     /gene="rnpB"
                     /locus_tag="VFH05_01260"
     ncRNA           complement(7525..7948)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFH05_01260"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(8023..8895)
                     /locus_tag="VFH05_01265"
     CDS             complement(8023..8895)
                     /locus_tag="VFH05_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6676874.1"
                     /translation="MYDVRMKRHAERIIGTILSLSLAFASGCETNPYTGRSQLLMTSV
                     SQEMQLGAQAYNQVKNDPKMKPSQDSREIEPVKRVAARIVEAAKRSKYAEMAQQFQWE
                     VTVIKDDKTANAFALPGGKMAVYTGIFPMAKTEAGLAAVMGHEVVHALARHGAERMSQ
                     GQLTSAALQVAGAAAGASGMNPLLSQGTMAALGAGAQVGVLLPFSRKHESEADYIGIL
                     LAADAGYDPRESVALWERMAQMSGGGGPAEFMSTHPSNETRIDQLKKWLPEAMTIYQS
                     KQPVPAAELPPVGR"
     gene            9105..9758
                     /gene="rnhC"
                     /locus_tag="VFH05_01270"
     CDS             9105..9758
                     /gene="rnhC"
                     /locus_tag="VFH05_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET6676875.1"
                     /translation="MPSSSIERIGIDESGKGDYFGPLVIAAVFVDATTQEELTLMAVR
                     DSKKISDGRILELAPDIKTICPHSVIAIGPQKYNELYGKIRNLNRLLAWGHAKALETL
                     LERVSCERAISDQFGDERLILNALQEKGRRIVLEQRTKAESDLAVAAASIVARAEFLL
                     RLKRLSDEIGTVLPKGASPAVELAGRMIVKKHGQERLGTVAKLHFKTTKAVLEKTAT"
     gene            complement(9771..10544)
                     /locus_tag="VFH05_01275"
     CDS             complement(9771..10544)
                     /locus_tag="VFH05_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET6676876.1"
                     /translation="MNQNLSPLIELQKIDLRIMEITEQRRRIPERLHAVETPLREAKQ
                     LLQDTSSAVEASVKERRSHEKDVEAQDAHTDKMRARLSELKTNKEYQAHLFELQMANK
                     KKSDIEDKVLACMEKIEQLQVAAKEAQEKVRIAEQAFTKEKQVLDEMDRKLSSELAEL
                     ESQQRERTAKVEPALLARYAKLKASRKDQPLAAIQRGMCAGCRLQIPPQLIAEVKRSQ
                     DLHTCPYCHRMLYWDGEAQADQKPLDHTKPTDLEVGESV"
     gene            complement(10604..10680)
                     /locus_tag="VFH05_01280"
     tRNA            complement(10604..10680)
                     /locus_tag="VFH05_01280"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10644..10646),aa:Ile,seq:cat)
     gene            complement(10827..12641)
                     /gene="rpoD"
                     /locus_tag="VFH05_01285"
     CDS             complement(10827..12641)
                     /gene="rpoD"
                     /locus_tag="VFH05_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET6676877.1"
                     /translation="MAKQELLVEVKKLISIGKEKGFLTYDELNSTLPAEVVSSEQFGS
                     IMTMFGEMDIEIVDAPEGDRAQKSRSAVPDQEEAGEDTEDSEAEDNEKEIDLTPGALS
                     RTDDPVRLYLKEMGSVALLSREGEIEIAKRIEEGKRDIAFVIYGMPMTIEFILALRDQ
                     LKNGKIDVREIVPIQETEEDFEEEEVERDYEELRLKTLEGLNTVRKVSTSLKGLYEKS
                     RQAGKDPAKLKKIKKQIDAIREQVVDKMEAVNLHGVLKDRMVQRVREMAIQIRTAERE
                     IVSCQRRIGVGGEAGAELLRRLCRDRKDFQAVKRKTGVSEDTLQDVKKLYQAAKSRIR
                     QLETEEALVSGEEIKDAVKHLDVAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFL
                     DLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETI
                     NKLIRTSRHLVQKLGREPTPEEIAERMDLPLDKVRKILKIAREPISLETPIGEEEDSH
                     LGDFIEDKKAISPLEAAIRYDLQRQINSALETLTPREEKVLRKRFGIGEATDHTLEEV
                     GQDFEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESL"
     gene            complement(12644..14467)
                     /gene="dnaG"
                     /locus_tag="VFH05_01290"
     CDS             complement(12644..14467)
                     /gene="dnaG"
                     /locus_tag="VFH05_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET6676878.1"
                     /translation="MGRGLISDDVINQIKDRIDIAEIVGQHVSLTRAGQNLKGLCPFH
                     QEKTPSFTVSSSRQIFHCFGCGAGGNVFTFLTRITGASFPETVKDLGRKVGIEIQDTV
                     SASEPQIAQGHRLEQLNQMAAQWFQQNLQNDRSGAEARAYLAGRGIQEATIERFGIGV
                     APVEWDGLIRWLGKQGCTQQDMAAAGLIIARDNGTGYYDRFRARVMITITDLRKRIVG
                     FGGRVLGDGIPKYLNSPDTPLFKKGQTLFALDVAREAVSRTKTVIVVEGYFDAIALHQ
                     AGLTHTVATLGTALTPDHVQMLRRFASKVVLLFDPDAAGVRAALRGLDLFVNSGLGVK
                     VVTLPAGEDPDTYVRKEGIDAFTRLEQQAPSLLDFALEQSVKTAESSTMEGRIQSVDE
                     ILRILQKSEHPIEREERIRLVAERFGINQQRLIDRYPALREKHPSRSAVSKSETPPVA
                     SSKGAPEERDLIYLLLHGHLSAADVRRLRPEAFSMPACRRIVETALVHLEPDGRVGLR
                     RLLDVVVDDPDCGPLATELSMQEQHFDDARAHIQGCLETIDRKRTEAMLRDLITQLKT
                     AEREGRIEDVRVLNGRVNEMRLQKAGRPLGVLSLVKGVSKE"
     gene            complement(14589..14933)
                     /locus_tag="VFH05_01295"
     CDS             complement(14589..14933)
                     /locus_tag="VFH05_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET6676879.1"
                     /translation="MSDCLFCQIVQKRIPAKIVHEDEHTLAFDDVNPQAPVHVLIIPK
                     QHVQAVGDVRETDSGLLAQLLLTCTKVAKHKGLALSGYRIVTNTGPDAGQTVFHLHLH
                     VLGGRHMTWPPG"
     gene            complement(14944..15597)
                     /gene="hisIE"
                     /locus_tag="VFH05_01300"
     CDS             complement(14944..15597)
                     /gene="hisIE"
                     /locus_tag="VFH05_01300"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HET6676880.1"
                     /translation="MTTRDFKFDVTGLIPAIVQDWLDGTVLMLGYMNEAALTKTLSTK
                     SVHFWSRSRQTIWEKGETSGNKLLVKEVFVDCDRDTILVKAQPLGPTCHTGERACFFS
                     KLEGDAEEHPSSEAFGAILESVLRIIVHRRAQPQPDSYTTKLFEGGQDTILKKVAEEA
                     GEVLLAAKGGKREEIIYEVADLFFHTLMVLGYHDVSLQEVYQELGSRYGKSGLKRKQ"
     gene            complement(15698..16465)
                     /gene="hisF"
                     /locus_tag="VFH05_01305"
     CDS             complement(15698..16465)
                     /gene="hisF"
                     /locus_tag="VFH05_01305"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET6676881.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVKLRDAGDPVEVAAVYDREGA
                     DELCFLDITASHENRKTIIDVVERTAARVFMPVTVGGGVRTLDDIRHLLNAGADKVSI
                     NTAAVERPEFVREAAERFGTQCIVVAIDAKRSGSSSWEVFTHGGRKASGLDAIDWAQS
                     MQRFGAGEILLTSMDEDGRQCGYDLDLTASVSARVSIPLIASGGVGSLEHLYDGFVTG
                     KADAVLAASIFHFRTFTIPQAKAYLHQRGVPVRVTGL"
     gene            complement(16495..17226)
                     /gene="hisA"
                     /locus_tag="VFH05_01310"
     CDS             complement(16495..17226)
                     /gene="hisA"
                     /locus_tag="VFH05_01310"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HET6676882.1"
                     /translation="MVVIPAIDVKDGRCVRLRQGDMDAETVYGTDIPSMATRWVQAGA
                     TIIHLVDLNGAVEGEPRNLTQIEAVIRSAPVQVQVGGGIRNMTTVRRYFKSGIARIVL
                     GTGALKDRAFLQQACQEFPKRILLGLDARDGKVAVNGWTSLSETTATHLLKELAGFDL
                     GAVIYTDIARDGMLSGPNIAALREILTFSAFPVIASGGISTIEDLQALQSLGPRVEGA
                     IVGKALYDGKLNYREAVAALRRADG"
     gene            complement(17230..17976)
                     /locus_tag="VFH05_01315"
     CDS             complement(17230..17976)
                     /locus_tag="VFH05_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676883.1"
                     /translation="MHSIGEASRHCLTGTIALLLILGSACSGSKVTAQTSSELPRYHI
                     RSIALLPFTTLTTPQVRDAGDMYLSTPQSVQRSDISLGIPSNVEPQLRQTTRVPAFAA
                     EKVTQLFWSRLKDRQGLVILSPSETSRVAVKSSPETSKATPEAQAAQLAAKLQADAAL
                     MGQVLVYQERSGSRMGANPPATVGFEVKAVAADGQVLWVGNYYERQKPLTEDFLGFIQ
                     HGGGFVTAEELAQYGTEKVLKTFPFGAKQD"
     gene            complement(17969..18580)
                     /gene="hisH"
                     /locus_tag="VFH05_01320"
     CDS             complement(17969..18580)
                     /gene="hisH"
                     /locus_tag="VFH05_01320"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET6676884.1"
                     /translation="MIAIIDYGMGNLRSVWKAFESVGLQALVTRDPAAITSAGHVVLP
                     GVGAFGDCMANLERFDLVEPIRSAVQSGKPFLGICLGLQLLFTESEEFGAHKGLNIIP
                     GQVKRFVPDPALKIPHMGWNQVNVERPCPLFADIVSGSDWYFVHSYFVQPVDPMVTAA
                     TTIYGTPFVSAVWKDNIVACQFHPEKSQSVGLRLMKNFGAWRA"
     gene            complement(18803..19408)
                     /gene="hisB"
                     /locus_tag="VFH05_01325"
     CDS             complement(18803..19408)
                     /gene="hisB"
                     /locus_tag="VFH05_01325"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET6676885.1"
                     /translation="MKKDAAPRQASLHRATKETDIRVEWTLDGSGESKIDTGIRFFDH
                     MLELMAKHGFFDLTVQAKGDLDIDEHHTVEDVGIVMGKALHQALGEKAGIKRFGFASA
                     PLDETLAQVTIDLSGRPFLVYNVNLPDRKIKAFDLGLFEDFFQAFVTQGGLNLHINLL
                     YGRNPHHIMEAIFKATAKALDQATMLEERLAGKVLSTKGTL"
     gene            complement(19405..20688)
                     /gene="hisD"
                     /locus_tag="VFH05_01330"
     CDS             complement(19405..20688)
                     /gene="hisD"
                     /locus_tag="VFH05_01330"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET6676886.1"
                     /translation="MKIVASTDRAFLPTLKKVAGRSRVQGAAAEKPVRAILDAVERGG
                     DKAVLRFTKRFDNVSLATTQLKVSPEEIKESYYKIRKEEGDSLRFAAQRIMAFHERQR
                     TKTWMYQDGAATLGQVVTPLDAVGVYVPGGKAVYPSTVLMSAIPAKVAGVKRIVMCTP
                     PQKGGINPYLLVAADIAGVTEIYRVGGVQAIGALAFGTKTILKVDKIVGPGNIYVATA
                     KRMLYGTVGIDMVAGPSELLIVADNDAKPAHVAADLLCEAEHDEEAQVWLVTTSASLA
                     KETVSLVEKQLKGLQREKIAAKSVRKYSVVFVVTTIDEAIDVANEIAAEHLTLSVDEP
                     FDYLEKIRHAGALFLGRYTPPSVADYIAGPNHVLPTGATARFFSALSIHDYTKISNIV
                     YYTKEELSKVKDHLIRLASIEGFDAHAKSAQSRFT"
     gene            complement(20685..21389)
                     /gene="hisG"
                     /locus_tag="VFH05_01335"
     CDS             complement(20685..21389)
                     /gene="hisG"
                     /locus_tag="VFH05_01335"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET6676887.1"
                     /translation="MLTIALCKGKLIEPTLKLFAKAGYGAAQPAADSRRLLFPCPEIG
                     MTFLIVRPTDVPTYVEHGAADVGVVGKDVLLEQDCDVYEPLDLGFGACRIAVAAFRGQ
                     VPRDRLSSKIRVATKYPKITERYFNQRGVPVEIIKLYGSIELAPIVGLADRIVDLVET
                     GNTLKAHGLIEVECIARSTARLIVNRASLKLKHQAVAELLSKLRHACATPGPARKDGS
                     VKPARSATGKPKRMRT"
     gene            complement(21393..22652)
                     /gene="murA"
                     /locus_tag="VFH05_01340"
     CDS             complement(21393..22652)
                     /gene="murA"
                     /locus_tag="VFH05_01340"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET6676888.1"
                     /translation="MDKIVISGGHVLQGEVQVSGAKNSALPILASTILGGGECVISNI
                     PRVVDVVTMGKLLGILGADVHHEGNRAVIKADVIRSTQAPYELVKTMRASVLVLGPLV
                     ARWGEAVVSLPGGCAIGSRPVNLHLAGLAKLGAEVSIEHGYIRAKAKRLKGAPIYCDV
                     PTVTGTENLMMAASLAEGTTVIENAAKEPEIVDLANFLVKRGARIAGAGTDVITIEGV
                     RELRGSDHEVIPDRIEAGTYVVAGAITKGSISVNRCRPEHLEPLLNKLGEAGVSIRVE
                     KERVHIDASKRGRLKGVDVRTLPFPGFPTDMQAQMVALMSVAEGTSIITETVFESRFM
                     HVEELRRMGADIRVEGNRVVITGHERLAGAPVMASDLRASAGLILAGLAAEGTTDVLR
                     VYHLDRGYERIEEKLRALGAVIDRQKG"
     gene            complement(22670..23569)
                     /gene="prmC"
                     /locus_tag="VFH05_01345"
     CDS             complement(22670..23569)
                     /gene="prmC"
                     /locus_tag="VFH05_01345"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET6676889.1"
                     /translation="MSPSVQHKAVHRPTLGSAVQDMRRRLEAAGIDSAEQEALWLIEH
                     ALALTGLHQIVDRERVLTDPEIAKLHDVATRRVAREPLQYILGTQEFCGLEFEVNPTV
                     LIPRPETELLAHEIIRRLPQGTPPTLMDVGTGSGCLAVTLARSIPRARILATDISRHA
                     LETAMRNARRHGVDSSITWLHGDLLGPLRGLGLEGSMHAILANPPYIRESEWAGLQPE
                     VSRYEPRLALVAGPRGTEVHERLLREAVSYLMPGGWLAMEVGEGQSLELCSKIAAMPD
                     YERVEVVPDEAGINRVVIVRRAR"
     gene            complement(23566..>24518)
                     /gene="prfA"
                     /locus_tag="VFH05_01350"
     CDS             complement(23566..>24518)
                     /gene="prfA"
                     /locus_tag="VFH05_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET6676890.1"
                     /translation="ELEGVAVLFRRYQDVLRQLEEAAHMSSDPAAGAELHSLAAEETA
                     QLEMEREALEAQVLDHLTPKDPRDDKNLFLEIRAGTGGDEAALFAGDLFRMYVKFAER
                     RKLKVDMVEASETGKGGYKTVIAVIEGKGGYGLFKYESGVHRVQRVPVTEAAGRIHTS
                     TVTVAVMPEVDEVDVHIDPKDLRIDTFCSSGAGGQSVNTTYSAVRITHIPTGVVVSCQ
                     DERSQLKNRTKAMRTLRARIVEAERERQEAEIAQTRKAQVGTGDRSEKIRTYNFPQNR
                     VTDHRIGLTLHKLEQVLEGDMDELVEALRAQRDHAAAEAS"
BASE COUNT         4959 a         7505 c         6505 g         5549 t
ORIGIN      
        1 ctatattctc gagcgattgt ttactaccgg gcggtacgta gagggtcgtg ccggcttcca
       61 cggtcgtgac ctgctcgtca atcatgaacc gtccatgccc ggtcagaaaa taatagactt
      121 cggatgaact caggacatgc cacttcgaac gctggccggg ccctaaagtc ccgtgtgcga
      181 gactatatcc aagtttcagc ggatgcttgg ccggatggaa cacttcacga agccgcgtat
      241 ggtcgccggc gaggaactcg ggccgatcag ccggcgtgac cttgaacaca gcagccccct
      301 tcgccactat caggcttggt aaagagggca ctgtaccgtg cgaggtttca tcaaatcaag
      361 tttaccttct gctccccctg cgcctgtttc gtagccaaat aggccagctt gttcaaggca
      421 ttcagggacg cccttgccga ggcggtaatg atatcggtgt ccgcgccatg gcccgacacg
      481 gttcgtccat cctcctgaac tctgacggag acttcgcctt gggcatcggt cccgccggtg
      541 atcgccttga cgacatacat gagcagcgtg cttttcgtct gggtaatggc ggcaattgtc
      601 cggtagacag catcgacggg gccatcgccg gtcccccttt ggctgagaga cttgccgtcg
      661 atttccaatt cgactgtggc ggtcggcacc tgatcggttc cactgctcac gctgaaggat
      721 ttcaaggtga tccgatcggc cattttggcc aattcctcgg agacaatcac ctcgagatct
      781 tcctcgtaga tctccttctt atgatcggcg agcctcttaa agcgctcgaa ggcatggttc
      841 acctcttcct ctgtcaactt gtaccccaat tcttcgaggc gctggcggaa cgcatggcgc
      901 ccggaaagct tgcccatgac cattttgctc tcgaccaagc cgatcgactc gggccgcatg
      961 atctcatacg tcgttttgtc cttgagcaat ccatcttgat ggatgccgga cgtatgggca
     1021 aacgcattgg ctcccacgat cgccttgttc ggctgcacca ccatgccggt aattttgctg
     1081 accagtcgac tcgtcttgga gatttcttcc gtgtgtattc tggtatcggc ctgatagagg
     1141 tctttccgcg tacgcaaccc catcgcaatc tcttccaatg aagtattgcc ggcgcgctcg
     1201 ccgatcccgt tgatcgtgca ctcgacttgg ccggcgccct ccatgactgc cgcgagacta
     1261 ttggccacgg ccagccctag atcgttgtga caatgcactg aaatgaccgc cttgccgctg
     1321 ttggctaccc gttccctgat ccctttgatc agcttcccga attcctgagg gatggcatac
     1381 cccacggtgt cagggatatt gaccgtcccc gcgcccgctt cgatgacggc ttcgatcacc
     1441 tcgtgcagat aggcgggatc agagcggctt gcgtccatcg gtgaaaactc gacgtcttcc
     1501 acgtaacccc tcgcccgctg taccatttcg accgcgcgct tcttggcttc ctcacgcgtc
     1561 atgcgaaatt gatgcttcag atggatgtcc gacgtcgaga ggaacgtgtg tatacgaacc
     1621 ttcggcgcac ctttgagggc ctcccaggca cggtcgatgt cctcggggcg agcccgcgca
     1681 aggctgcaaa tcgtcggccc ttccacctcc tgggcaatgc gccgcaccgc ttcgaaatcc
     1741 ccgggagaac tataggcgaa cccggcctcg ataatgtcca ctccaagcct ggccagctgt
     1801 ttcgccacca tcactttttc ttccacgttc atactcgcgc cgggagattg ctccccgtca
     1861 cgcaatgtgg tgtcgaaaat tctgatcatc cgtgtcatgt tctcacctct tctggtctaa
     1921 gtggtccact cgacgcgagg ccgagcaaaa cggatttctg caaagcggca gcgagcgaaa
     1981 cggtgaggcg tacgtttgac ggtacgtcga gccgttgagc gacggaagca cgatgcagaa
     2041 atccactttc atcggcctct aaaagcaaaa gcctccatcc cttggcccag ggacggaggc
     2101 ttcgaagact ccgtggtacc accctgattc ggctcggacc tcactctcgt tgtccgatgc
     2161 ccttcattcg gcccgtcacg agggcaaggc ggaatccatt actcggattt cgtggatcca
     2221 ggctcggagg cgagttcagc aagatttggg ttggctcgca ccatccgcca actctctatc
     2281 ctgaatgttg gagccagccc ccagctccgt tctcatcgtt caaaggcgct ggctgcccgc
     2341 tttcaacatt ctggtgtcca aaccgttgct tactactcct cgtcgttgcc gctaccactt
     2401 ctgttcgacg acttctcgtt tcgacggggg ttgcttaccc gcgaatttcg tgagcacaac
     2461 gaacatgcgt tcgaccggcc caagcagggc atacccggcg aacatgacaa atagcataac
     2521 ctgaggccac gccgctacaa gaactaacgc aagaatgccc cacaccaggt aagtgatgtg
     2581 ctgtcgtcct cgaaatttca ggtccttgaa gctgcggtac ttaatggtgc tgaccataag
     2641 gaacgataag atcagcgtca cgatcagtac cgcgaccggc ttgacctccg cgcccacccg
     2701 aacaatgtag tggtcgaaaa tcaccatact ggccacgact cccgcggcag cgggaatcgc
     2761 caggcccgta aaatacttgc cgtcggccat cgttgtagtt gcattgaacc gggcgagccg
     2821 aaccgctccc atggccacgt aggcgaacat aacggcgatg ccgaatgtgc cttggccgct
     2881 caaggcccac gaatagagca agagtccggg tgcgacgccg aaggacacca gatcggacag
     2941 ggagtcgtat tccaacccaa agtggctggt gctgttcgtc atccgagccg attttccatc
     3001 cagaacgtcg aagatcatgg cgacgaggat ggcaatcgcc gccgccatat aatcggcatt
     3061 gaagacggac aagacggcaa aaattccgca gaacagattg ccggtcgtaa atagattcgg
     3121 gatcaggtgc atggccgcct tgtgccgttt ggtgcctttg gcaaaggatt gtctaagacc
     3181 ggtgtttttc attgtagttc ccccaggatt gtttctccgc ctgtcacacg gttaccgaca
     3241 gcaatcctga ttgccgtccc gaccggaaga tacgtatcca tccgagagcc gaaccggatg
     3301 agaccatagc gctcgccacg aaccgccctg tccttggggt tcacccagca cacgatccgt
     3361 ctggcaatga gtcccgcaac ctgcacgcag agcactttgg ccccttccac cgtcttaatc
     3421 atcacggcgt tttgctcgtt tttcactgtc gcatggggct tactggccac cagaaacaac
     3481 ccgggctggt actgtacatt ctcgacaatg ccttcacacg gcatccggtt gatgtgcacg
     3541 tcgaacacgt tcaagaaaat ggagatccga acggcgcgat ctttcaggta ccgcggctcg
     3601 aattcttctt cgaccgcaag cacgacccca tcacccgggg caacgacgag tccaggccct
     3661 tggggaaccc ttcgcggcgg gttgcggaaa aaccaggaca cgaagagggt cagcatggcc
     3721 cccacgaccg ccacgggtat ccaaccgagc caccatgcga tcagtgtaat ccccgcggca
     3781 gcgccgatga agggaaatcc ttctttggca aagggaacac cggccgctcg atcagtcaca
     3841 ggcctccacc tccatcaatt ttttgactta tcaaccaact gatccttctt gagccatggc
     3901 atcatcgctc gcagcttggc accgacttct tcgatcggat gcgcctcacc tttggccaac
     3961 agcgcgttgt acacggggcg attcgcctga ttttccaaga cccattcctt ggcaaattgc
     4021 ccctgctgga tttcgccgag gatctttttc atctcctgtt tagtctgttc ggtgacgata
     4081 cggggacctc tcgtcacatc accgtattta gccgtcgtgc tgatcgaata ccgcatattt
     4141 gcgatcccac cctgatagat caagtcaacg atgagcttca cttcgtgcag acattcaaaa
     4201 tacgccatct ctggcgaata gccggcttcc acaagggtct catagccggc ctgtatcagc
     4261 gaagtcagac cgccgcagag gaccgcttgt tcgccaaaca ggtccgtctc ggtttcttca
     4321 cggaaattgg tttcgatgac gccggcgcgc cctccgccga tggcacttgc ataggccaga
     4381 cccacctgct tggtggtgcc gctgggatct tgatgcactg ccaagagaca ggggactccg
     4441 ctgcccttgg tatactcaga tcgtaccaaa tggccggggc ctttgggagc gaccataaac
     4501 acattgatcg tcggcggggc gacgatctgc ccgaaatgga tattgaatcc atgcccgaac
     4561 gcaaggtacg aacccgcctt caagttcggc gcgacctcct gccgatagag ggcagcctgt
     4621 gcttcgtcag gcgccaagat catgacaaca tcggacgctt tcaccgcatc cgctacgggc
     4681 atgaccttta gtccgctctg ctcggctttg cgccaggaac tgccttcacg caagccgacc
     4741 acgaccgaga cgccgctttc tttcatgttg agggcgtggg catggccttg gctgccgaag
     4801 ccgatgaccg ccactttctt gttggtaatc tgttgaagat cggcatccct gtcatagtaa
     4861 atcttcatca tgatggactc ccgaaggcaa aaggttgatg cgtgacggca ggcggggaca
     4921 cccgcctcat tactcgcggg cgactttctt gggttgcgtc gtcgtggaac ggagcggttc
     4981 ccgggcaacg gccacccggc cggtcctggt caattctttg attcccagcg gctgaaggag
     5041 attgatgatc gcttcgatct ttttcggatc acccgtgact tcaatcgtat aagtcgcggg
     5101 agtcgaatcg atcacattcg cgcggaagat atcggcaatc cgtaaggctt ccgcgcggtc
     5161 ttcctgcctg gtgtgcactt tgatcagcgc cgtctcgcgc gagacaaaat cgctttcgtt
     5221 caaatccacg actttgatga cgtcaatcaa cttgttgagc tgcttgacaa tctgctcgac
     5281 gatccgctca tcacccgagg tgacgatcgt catttgagac atggacggat ccaacgtcgg
     5341 tgcgacagat aggctttcga tgttgaaacc gcgaccgctg aacaacccgg cgatccggga
     5401 caggactcca aacttattct cgaccgtcac taaaatgatg tgttccatcg tcctggtctt
     5461 atcgtcggtg ccggcatgac gctgcggatc tcggcaaccg tccaaaatcc tctatgccgt
     5521 caagatcgtg tctttgtcct gtggagtcac cttggcgccg ccgctctgtt tcagtttcag
     5581 ctccggcgga tcgtccaaaa tcatctcgtg attgcacccg cctgccggaa tcatggggta
     5641 gacgttttcg tagggatgtg tcggcacgtc cacgatgacc ggccctttgg ccgcaatggc
     5701 ttcctgcagc acgttgtcca aatcgctcac cttcgacggc cgcaaaccta tggcgccgta
     5761 cgcttcggcc agcttgacga aatccggggt ggtgtccaga taactcgagg catagcgtcc
     5821 ctcgtagaag aggtcctgcc attgccggac cattccgtga aatcgattgt ttaagacgat
     5881 gattttcacc ggaagtttgt gcacgaccgc agtcgccatt tcctgcatgt tcatctgcac
     5941 gctgccgtca cctgcgatgc agaggacgag ccggccggga aaggcggctt gggcacccat
     6001 tgcggcggga aatccgaatc ccatcgtccc gagtccgccg gaggtcaacc aacggttcgg
     6061 cttggccatc ttgaaatatt gggccgccca catctggtgc tgaccgacgt ccgtggacac
     6121 gatgggatcc aggtccttcg tcagctcgta caggcgtttg atcacttgct gcggcttaat
     6181 gggtccatcc gcctcctgct gatagctgag gggatgcgcg ctctgccatt cccgaatctg
     6241 gtcccaccag gctttgcgca gttccttttg ctcgccgttt actgtcgccc ggagaatctg
     6301 attcaattcg cgcagtacgg ccttgcagtc gccgacgatc ggaatgtcga cgtggatgtt
     6361 cttcctgatc gacgtcggat caatgtcaat gtgaatgatc ttggcgtaag gacagaactc
     6421 ggacaccttg ccagtcaccc ggtcatcgaa ccgtgcgccg atggccacga cgagatccga
     6481 gtagtgcacg gccatgttgg cttggtaagt gccgtgcatt cccatcattc caagagacag
     6541 aggatgttca cccgggaagg cgcccaaccc catcaacgtc atgtcgactg gaatctgcgt
     6601 gagctcggcc aattcaatta actctttcga ggcacccgag aagatcacgc cgccgccgac
     6661 gtaaagaatc ggcttcttgg acttcataat ggcttctgcg gcctgtttga cctgccactt
     6721 attgccttca tatgtcgggt tgtatccccg gatcgacacg gaattcggat aattgaactc
     6781 cgccttgtcc atcgagacgt ctttgggaat gtccaccaaa accggcccag gacgcccggt
     6841 ggtggcaatg tagaacgcct ccttgatggt tgaggccagc tcgttcacgt ctttcacgag
     6901 aaagttgtac ttcgtgcagg gacggctcag tcctacgttg tccgcctctt ggaaggcatc
     6961 gtttccgatc aaattggtcg gcacctggcc gctaaagcac acgagcggga cagaatccat
     7021 gtaggcatcg gccaaggcgg taatcacatt ggtcatgccg ggtcccgacg ttacgagaca
     7081 cacccccgcc ttgccggtgg ccttggcgta tccttcggcc atgtggccgg caccttgctc
     7141 atggcgagtc aacaccacct ctatgtcctt ctgttggtgc agcatgtcga aaatcttgag
     7201 gacaaccccc cccggcagcg cgaagagggt tttcactcct tcacgcttga ggcattcgat
     7261 aaaaatttcg gcgcctgtca gtttcatgca gacctccaat ccctcccgtg gcccttgtca
     7321 gcttgacgct tttcgattct ggccatcgga ggtggataca ccattaccac tgatcaccta
     7381 ccgctgatca tcgagaaata tctgaaatat caatgacaaa gatcaaagat cctagcacca
     7441 cgataatgcc aggtcaatag acaggctcct aacacagcgg aggcggtgtt atgaagggtt
     7501 gagagcctgg acggtaagag agcgcggtgg agaggactta taagccggat tctgtcccgt
     7561 caaggatttg ttcctcaacg gtggtcatca tttctctggg atccgagtta cctcgaacct
     7621 caagcgacct acccgaagac ctcggccggg ccagccggtc gccgcttcaa aggaagcgac
     7681 agctgtcttc ctatttggtc ttgcatcgag cgacgcttac cgtgccggtg atgtcgccat
     7741 ccccgcggtg ggctcttact ccaccgtttc acccttacct gatccgcagc gattcgattg
     7801 gggtcccgtc gctttggcca tcggcggttt gttttctgtg gtgccggtgt cggatcgctc
     7861 cgcctgggcg ttacccagcg ctctgcccat gaagtccgga ctttcctctc gcgacgtcac
     7921 cgccacgagc gatcacctgg tcctctccct cgcgcagatt gagtatagga aatgaacgag
     7981 catgatgcaa gaaggagtct catcgatggt ctcaccggta agttaccgtc caaccggtgg
     8041 caactccgca gccggaaccg gttgctttga ctgataaata gtcatggctt cgggaagcca
     8101 tttcttcagc tggtcaatgc gtgtttcgtt actcggatgg gtggacatga actccgctgg
     8161 tccgccacct ccggacattt gggccatccg ttcccacaga gccaccgatt cgcgcgggtc
     8221 gtaaccggcg tcggccgcaa gcagaatgcc gatatagtct gcttccgatt cgtgttttcg
     8281 actgaacgga agcaacacgc cgacttgcgc tcctgctccc aacgccgcca tcgtcccctg
     8341 ggaaaggagc ggattcatgc cgctggcacc cgctgcggcg ccggccactt gaagcgccgc
     8401 actcgtgagt tggccctgac tcatccgttc cgccccatgc cgggccaagg catgcaccac
     8461 ttcgtgtccc atgaccgcag cgaggccggc ttccgtcttg gccatcggaa agatacccgt
     8521 gtacaccgcc atttttcccc cgggcagcgc aaacgcattg gcggtcttgt cgtccttgat
     8581 cacggtgact tcccattgaa actgctgcgc catctctgcg tacttcgatc gtttcgccgc
     8641 ctcgacgatg cgagctgcca cgcgcttcac gggctcgatc tccctggagt cctgtgatgg
     8701 tttcattttg ggatcgtttt ttacctggtt gtatgcctgc gcacccagct gcatttcctg
     8761 cgacactgag gtcatcagca attgtgatcg gcctgtgtag ggattggtct cgcatccgct
     8821 cgcgaatgct agcgacaaag acaggattgt gccgatgatg cgctccgcgt gccgcttcat
     8881 tctcacatcg tacatgactc gcacctccac gactcacaac gtgcttgcgg tcaatgggtt
     8941 tgaccgattt tcgctgctct gctacattag cgtctcgcac cgaggttttc cactgatgta
     9001 gcggatcccg gcaggatgct ggcgcatcca agcaggggcg ctgcctgcaa ctcgtcgccc
     9061 gcacgagcat cgactacctg ctcagcatca cacagatcaa caggatgccg tcctcttcga
     9121 tcgaacgcat aggaatcgac gaatcgggaa agggcgatta cttcggcccg ctcgtcatcg
     9181 ccgccgtgtt cgtcgatgcg acgacacaag aggagctcac gctcatggcg gtgcgagaca
     9241 gcaagaaaat ctcagacgga cgcattctcg agctcgcgcc tgacattaag acgatctgtc
     9301 ctcacagcgt catcgccatt ggcccccaga aatacaacga actctatggg aaaatccgaa
     9361 acctcaatcg tttgctggct tgggggcacg cgaaggcgct tgagacgttg ttggaacgcg
     9421 tctcctgcga gcgtgcgatc tcggatcaat tcggcgacga gcggctgatc ctcaacgcgc
     9481 tccaagaaaa ggggcggcgc atcgtgcttg agcaacgcac gaaggcagag tcggatcttg
     9541 ccgtcgccgc cgcctcgatc gtggctcggg ccgagttctt gttacggctc aagcgtctct
     9601 cagatgagat cggtactgtc ttacccaaag gggcatcgcc tgcagttgag ttggctggca
     9661 ggatgatcgt gaagaagcac ggacaagagc ggttgggaac ggtcgcgaaa ctgcacttca
     9721 agacgacgaa agccgttctc gagaagacag cgacgtagtg taaaagagac ttacaccgat
     9781 tctcccacct cgagatcagt cggctttgta tggtccagcg gcttttggtc tgcttgcgct
     9841 tctccatccc agtacaacat gcggtggcaa taggggcatg tgtgcagatc ctgagagcgt
     9901 ttcacttccg cgatcaattg aggagggatt tgcagccggc atccggcaca catgccgcgc
     9961 tggattgccg caagcggctg gtcctttctc gaggctttta actttgcata gcgggcgagc
    10021 aacgccggtt cgactttcgc cgttcgctcc cgttgctggc tctccagttc ggccagttcc
    10081 gacgacagct tccgatccat ctcgtcgagc acctgctttt ccttagtgaa ggcctgctcg
    10141 gcgattcgaa ccttttcctg tgcttccttc gccgccactt ggagttgttc gattttttcc
    10201 atacaggcca acaccttgtc ctcaatgtcg gacttctttt tgttggccat ctgcaactcg
    10261 aacagatggg cctgatattc cttgttggtc ttcagttcgg acaaccgcgc ccgcattttg
    10321 tcggtgtggg catcctgcgc ctcgacgtct ttttcatgcg agcgccgctc cttgaccgag
    10381 gcttcgacgg cactactcgt gtcctgtagc aactgtttcg cctcgcgcag gggcgtttca
    10441 accgcatgca gtcgttccgg gatcctccgg cgctgttcgg tgatctccat gatgcgaaga
    10501 tcaatctttt gcagttcgat gaggggagac agattctggt tcaatgcact cccaagtctc
    10561 aggttcagga aggaaggata tgcgattccc gccgccggtc cactggtggg cccactagga
    10621 ctcgaaccta ggaccagctg attatgagtc agccgctcta accacctgag ctatgggccc
    10681 catgggaaca gttggctccg gctcagggtg aggtcgagat tcggatcaga ctcgacagcc
    10741 ttccatcagc tcagccttga ccgcagcctc gacgtcgact gattttaggc ccgggcggga
    10801 gacagagtca aatcatccgt cggtcctcac aagctctcca cgaaactcct gagctttttg
    10861 ctgcggctgg gatgccgtag cttacgcaac gccttggcct caatctgacg gatgcgctca
    10921 cgggtcactt caaaatcttg tccgacttct tccagcgtat gatcggtggc ttcgccgatg
    10981 ccaaaccgct tgcgcaagac cttctcttca cgcggcgtca gggtctccag cgcgctgttg
    11041 atttgtcgtt gcaaatcata acgaatggcc gcctccagcg gagaaatggc cttcttgtcc
    11101 tcgataaagt cacccaggtg gctgtcctct tcttcaccga tcggcgtttc caacgaaatc
    11161 ggctcacggg cgatcttcaa gatcttccga actttatcga gaggcagatc catacgctcc
    11221 gcgatctctt caggggtagg ttctcggccg agcttttgca ccaggtgacg agacgttctg
    11281 atcagtttat tgatggtttc gatcatatga acgggaatac ggatcgtacg ggcctggtcg
    11341 gcgatggcgc gcgtgattgc ctggcggatc caccacgtgg cgtacgtact gaatttgtat
    11401 ccgcgcttgt attcgaactt gtcgacggcc ttcatgagtc cgatgtttcc ttcctggatc
    11461 aagtccagga actgcagacc gcgattcgta tacttcttcg cgatactgac gacgagccga
    11521 aggtttgcct caacgagttc cgccttgcct cgcttgacct tttcctccgc cacgtcaaga
    11581 tgcttgaccg cgtccttgat ctcttcgccg gaaaccaggg cctcctcggt ctccaattga
    11641 cggatgcggc tcttggcagc ctggtacagt tttttgacgt cttgaagggt gtcttcggac
    11701 acgccggttt ttcgtttgac tgcctgaaaa tctttccgat ctcggcatag tctcctcagc
    11761 agttcggccc ccgcttcacc gccgacgcca atgcgccgct ggcaactgac gatctcgcgt
    11821 tcggccgtcc gtatctgaat ggccatctca cggacccgct ggaccatccg atccttcaga
    11881 accccgtgca ggttcacggc ttccatctta tcgacaacct gttcccggat cgcgtcgatc
    11941 tgctttttga tcttcttgag cttcgccgga tctttccccg cctgacgtga cttttcgtac
    12001 agccccttca gcgacgtcga caccttccgg acggtattca agccttccag ggtcttgagc
    12061 cgcaattcct cgtagtcccg ctcaacttct tcttcttcga aatcctcttc cgtttcctgt
    12121 atcggcacga tctcacgcac gtcgatcttg ccgttcttga gttgatcgcg gagcgcgagg
    12181 atgaattcga tcgtcatcgg catgccgtag atgacaaatg cgatgtcccg ctttccctcc
    12241 tcgatgcgct tggcgatttc aatctcgcct tcgcggctga gcagtgcaac gctccccatt
    12301 tccttgaggt agagcctcac gggatcgtcg gtccgactca gcgcccccgg ggtgagatca
    12361 atttcctttt cattgtcctc cgcttcgctg tcctccgtgt cttcgccggc ctcctcttga
    12421 tcggggacgg cactccggct tttttgagca cgatcacctt ccggggcatc gacgatttcg
    12481 atgtccattt cgccgaacat tgtcataatg ctgccgaact gctcggatga gaccacttcg
    12541 gcgggcaacg tgctgttcaa ctcgtcgtag gtcaggaacc ccttctcctt accgatcgag
    12601 atcagcttct ttacttccac aagcaattct tgtttcgcca tgcttactcc ttacttacac
    12661 ccttcaccaa agaaagcact ccgagaggtc gcccggcctt ttgcaggcgc atttcattga
    12721 cacggccatt caacaccctg acgtcttcga tccgtccttc acgctctgcg gtcttgagct
    12781 gcgtgatcag gtcacgcagc atggcttcag tccgctttcg atcgatcgtc tcgaggcaac
    12841 cctgtatatg cgcgcgggcg tcgtcaaaat gctgttcttg catagacaat tctgtagcca
    12901 ggggtccgca gtctggatca tcgaccacga catccagcaa tcgacgaagt cccacccgac
    12961 cgtccggttc aagatggacc agcgcggttt caacgatccg gcggcaggct ggcatcgaaa
    13021 acgcttcagg ccgcaatcga cggacgtcgg cggcggacaa gtggccatgg agcaacagat
    13081 agatcaagtc ccgttcctcc ggagccccct tcgacgaggc aacgggcgga gtctcacttt
    13141 tggagacagc cgaacgcgac ggatgttttt cccgcaaggc cggataccgg tcaataagcc
    13201 gctgctgatt gatgccgaag cgctcggcca ccagacgaat ccgttcttcc cgttcaatgg
    13261 gatgctcgct cttttgcaaa atgcgcaaga tttcgtccac gctttggatc cgcccttcca
    13321 tggtactgct ctccgcagtc ttgacgctct gctcaagagc aaaatccagt aaactcgggg
    13381 cctgttgttc caagcgggta aaggcgtcta ttccttcttt tcgaacgtac gtatcgggat
    13441 cttctccggc gggcaatgtc accaccttca cgcccaatcc gctgttgaca aagagatcca
    13501 accctcgcaa cgccgcccgg acaccggccg catcgggatc gaacagcaac acgacttttg
    13561 aggcgaaccg ccggagcatc tgaacatggt cgggagtgag cgcggtccca agggtcgcta
    13621 ccgtatgtgt caacccggcc tggtgcagcg cgattgcatc gaaatagcct tcgacgacaa
    13681 tgaccgtctt cgtccgactg acggcctcgc gggcgacatc aagggcaaaa agcgtttgcc
    13741 ccttcttgaa cagcggcgta tccggcgaat tcaaatattt gggaatcccg tcgccaagaa
    13801 cgcggccacc gaatcccacg atacgcttac ggaggtcggt gatggtgatc atcactcgtg
    13861 cgcgaaatcg gtcgtaatac ccggtcccat tgtcccgcgc gatgatcaag ccggctgccg
    13921 ccatgtcttg ctgggtacac ccctgcttac cgagccacct gattaatcca tcccattcca
    13981 ccggcgcgac accgatgccg aaacgctcga tcgtggcctc ctgaatgcct cgccctgcca
    14041 gataggctcg cgcctcagct ccggaccgat cgttctgcaa attttgctga aaccattgtg
    14101 cagccatctg gttgagctgt tcgagacgat ggccttgggc gatctggggt tctgaagcgg
    14161 agacagtgtc ctgaatctca attcccacct tgcgaccgag atccttgacc gtttctggga
    14221 aacttgctcc ggtgattctg gtcaaaaacg tgaacacatt gcctcccgcg ccacagccaa
    14281 aacagtgaaa gatctgtcgg ctggagctga cggtgaacga tggagttttt tcttggtgga
    14341 aggggcagag acccttcaaa ttctggccag cacgggtcaa gctgacatgc tgcccgacaa
    14401 tctcggcgat gtcgatccga tccttgattt gattgatgac atcgtcagaa atcaggcctc
    14461 gacccatggg agacgcagct cctcactccc ccaaacgcgg tggggctggt atgcgtaagt
    14521 gatcggaaaa attgaccggt caggttaaca gagacacggg aaacctgtca aacacgagac
    14581 gatgacgatc atcccggcgg ccaggtcatg tgtcgtccgc ctaacacatg tagatggagg
    14641 tgaaacaccg tttggcccgc gtcggggcct gtattggtca cgatacggta gcctgacagc
    14701 gcgaggccct tgtgtttcgc gaccttggtg caagtgagca acagttgagc caagagtccg
    14761 gaatcggtct ccctcacgtc tcccaccgcc tggacatgtt gcttcggaat gatcaggaca
    14821 tgaacaggcg cctgcggatt gacgtcatca aaggccaatg tgtgctcgtc ctcatggaca
    14881 attttggcag gaatccgttt ttggacgatc tgacaaaaca ggcagtcact catcgggaat
    14941 gcctcattgt ttccgtttga gacccgactt cccatatcgt gaacccagct cttgatacac
    15001 ctcttgcaga gagacgtcgt ggtaaccaag caccatcagg gtgtggaaga acaaatcggc
    15061 gacttcatag ataatctctt ctcgcttgcc cccttttgca gcaaggagaa cctcgccggc
    15121 ttcctctgcc acttttttga gaatcgtatc ctgcccgccc tcgaagagct tggtggtgta
    15181 ggaatcgggc tgtggctggg cgcggcgatg tactataatc cgcaggacac tttcaagtat
    15241 tgcaccgaac gcttcggatg acgggtgctc ctcagcgtct ccctccagtt tcgagaaaaa
    15301 gcacgctcgc tctcccgtgt ggcaggtggg gccaagcggt tgtgccttga ccaggatcgt
    15361 atcccggtca cagtccacga agacctcttt cacgagcaac ttgttgccgg acgtttcacc
    15421 cttttcccat atggtctgcc gcgagcggct ccaaaagtgc actgatttcg tcgataacgt
    15481 tttcgtcagc gctgcttcgt tcatgtatcc gagcatgagg acggtgccat ccagccagtc
    15541 ctgcacgata gccggaatca ggcccgtcac gtcgaatttg aaatcgcgag tggtcatgtc
    15601 cgccacacat ccttcgcctg tttgagacaa gcgccgtcat aggccgctgc attgcgagag
    15661 atcagctgga ccctctcacg gccgcgagaa cggttattca cagtcctgtc acacgcacag
    15721 gtactccgcg ctggtgcaaa taggccttgg cttgcggaat cgtaaaggtc ctaaaatgaa
    15781 aaatcgaggc cgcgagcacc gcatccgctt taccggtgac gaaaccgtca tagagatgtt
    15841 ccagcgaccc gacgcctccg gatgcgatca gtgggatcga cacgcgggca gagactgaag
    15901 ccgttaaatc cagatcgtag ccacattgcc ggccatcttc atccatactg gtcaataaga
    15961 tctcgccggc cccaaaccgt tgcatgctct gcgcccagtc gatggcatcc aaaccgctcg
    16021 ccttccgtcc gccgtgcgtg aacacctccc aggacgacga tcccgatcgc ttggcgtcaa
    16081 tcgccacgac aatgcactgc gtgccaaacc gttcggctgc ctccctcacg aattccggcc
    16141 gctccacggc agcggtattg atactgacct tgtccgcccc ggcattcaac aagtgacgga
    16201 tgtcgtcgag tgtccgcacc ccgccgccga ccgtgaccgg catgaacacc ctcgcagccg
    16261 tccgctccac cacgtcgatg atcgtctttc gattttcgtg ggacgccgtg atgtccagaa
    16321 agcacagctc gtctgcaccc tctcgatcat agaccgccgc cacctcgacc ggatcgccgg
    16381 catctcgaag tttgacaaag ctcacgcctt tgacgacacg gccgtctttc acgtcgaggc
    16441 agggaatgat gcgcttggtc aacatacctt gtactgcccc tagacacgtg ccggctaccc
    16501 gtcagcacgg cgaagggcgg cgacggcctc ccgatagttc aactttccgt catacagcgc
    16561 ctttcccacg attgcgcctt caactcgagg gccgagcgat tggagagctt gcaggtcctc
    16621 gattgtggaa ataccgccgg aggcaatcac gggaaaggca gaaaatgtca ggatttccct
    16681 caaggcagca atgttcggtc cgctcagcat gccatctcgc gcaatgtcgg tataaatgac
    16741 ggcgccgaga tcgaaccccg ccaactcctt gagtagatgc gttgccgtcg tctccgacag
    16801 cgaggtccaa ccgttgactg ccaccttgcc gtcgcgagca tccaagccga gcaggatccg
    16861 cttgggaaac tcttgacagg cctgttgcaa gaaggcgcga tccttcaatg cccctgtgcc
    16921 cagcacaatg cgggcgatgc cggacttgaa ataccttctg accgtggtca tgttccgaat
    16981 gccgccgccg acttgcacct ggaccggcgc cgaccgaatg acagcctcga tctgcgtaag
    17041 attccgaggt tctccttcta cggccccgtt cagatcgacg aggtgaatga tcgtcgcgcc
    17101 ggcctggacc catcgtgttg ccatcgatgg aatatcggtg ccatacacgg tttcagcgtc
    17161 catatcgccc tgacgcaacc ggacgcatcg gccatccttc acatcaatcg ccggtatcac
    17221 caccatgggt tagtcttgct tcgctccgaa cggaaacgtc ttgagcactt tctctgtccc
    17281 atattgggcg agctcctcgg cagtcacaaa tcctccacca tgctggatga accccagaaa
    17341 gtcctctgtc agcggcttct gccgctcata ataattgccg acccagagca cctgtccgtc
    17401 ggccgccacg gctttcactt caaagcctac tgtcgcggga ggattggctc ccatacggct
    17461 tcccgatcgt tcttggtaga ccaagacctg ccccatcagt gcggcgtcgg cttggagctt
    17521 cgctgccagc tgagctgcct gggcttccgg cgtcgccttc gaggtctcag gggaggactt
    17581 gaccgccacc ctggaggttt cgctgggcga aagtatgacg aggccctgcc gatctttcag
    17641 tcgcgaccaa aacagctgtg tcaccttctc agccgcaaag gccgggaccc tggtagtttg
    17701 tcgcaattgt ggttcaacgt tggacgggat gcccaaggaa atatcagagc gttggacact
    17761 ttgcggcgtt gaaagataca tgtctcccgc atcacggacc tgtggagtcg tcaaagtcgt
    17821 aaagggcaat aacgcaatgc ttcggatgtg ataccgaggc agttcactcg acgtctgcgc
    17881 cgtgaccttg gaaccgctgc aggcggagcc gagaatcagc aacaaggcga tggttcctgt
    17941 caagcaatga cgtgatgcct cgccgatgct atgcacgcca ggccccaaaa tttttcatca
    18001 aacgcaagcc cactgattga cttttttccg gatggaactg acaggccacg atattgtcct
    18061 tccatacggc cgagacgaat ggagtgccgt agatggttgt cgcggctgtg accatcggat
    18121 caaccggctg gacgaagtag gaatgaacaa aataccagtc agaacccgag acgatatccg
    18181 caaacagcgg acaaggccgc tcgacgttga cctggttcca tcccatatgt gggatcttca
    18241 gggcgggatc cgggacaaac cgcttcactt ggccgggaat gatattgagt cccttgtgcg
    18301 caccgaactc ctcactttcc gtaaacaaca gttgaagccc caagcagatg cccagaaagg
    18361 gcttgcccga ttgaaccgct gatcgaatgg gttcaaccag atcaaatcgc tccaagttgg
    18421 ccatacaatc gccgaaagcg cccacaccgg ggaggaccac atggccggca cttgtaatcg
    18481 ccgcgggatc acgcgtcacc aacgcctgga gccccaccga ctcgaaggct ttccagacgc
    18541 tgcgcaaatt tcccatcccg taatcaataa tcgcaatcat gggccaccct gagcctcggc
    18601 tcctaaccat accgcaatcg gctgataaaa cagaaggaca ggtttcccga aggaacctgc
    18661 ccacaccggc gcgcattgtt ctgcgatgat cagatggcac ggacgacgaa gataacgcca
    18721 tgatccgctg cagggcgtga tgccgtgtca ccccgtcgat aggaatgcat ggagatatgc
    18781 acgagacagg acttgcgacg ccttataacg tgccctttgt cgaaagcact tttcccgcca
    18841 atcgctcttc caacatcgtc gcctgatcaa gagctttcgc tgtcgctttg aagatggctt
    18901 ccatgatgtg atgcggattg cgtccataca acagattgat atggagattt aacccgccct
    18961 gggtcacgaa ggcctgaaag aagtcttcga agaggccgag gtcgaatgct ttgattttgc
    19021 ggtccggcaa attgacgttg tacacgagga acggcctgcc gctgagatca atggtgactt
    19081 gtgccaacgt ctcatccagt ggcgccgaag cgaagccgaa tcgcttgatg ccggcctttt
    19141 ctcctagggc ctgatgaagc gccttgccca tgacaatccc gacatcttcc actgtatggt
    19201 gttcatcaat gtcgagatca cctttggcct gaacagtgag atcaaaaaat ccatgcttgg
    19261 ccatcaactc cagcatgtgg tcgaaaaacc gaatgcccgt gtcgatcttg ctctctccgc
    19321 tgccgtccaa cgtccactcg acgcgaatgt cggtttcctt ggtcgctcga tgcagcgagg
    19381 cctgtctggg ggcggcatct tttttcatgt gaacctgctt tgggcggatt tggcgtgcgc
    19441 atcgaaccct tcgatggacg ccagccggat aaggtgatcc ttgactttgc tcaattcttc
    19501 tttggtgtaa tacacaatgt tgctaatctt cgtataatcg tggatcgaca gagccgaaaa
    19561 aaacctcgcc gtggccccgg tcggcaagac gtgattcggc cccgcgatgt aatcggcgac
    19621 ggaaggcggg gtatagcgcc ccaggaacag tgcgccggca tgtcggattt tctccagata
    19681 atcaaacggc tcgtctacgg acagggtgag atgttcggcc gcgatctcgt tcgccacatc
    19741 gatcgcttca tcgatcgtcg tgaccacgaa aacgacggaa tatttcctga cggacttggc
    19801 cgcgattttc tcacgctgta atcccttcag ttgtttttcg acaagagaga ccgtctcttt
    19861 ggcaagcgag gcggacgtcg tcaccaacca gacttgtgcc tcctcatcgt gctcagcctc
    19921 gcagagtaag tcggccgcca catgcgccgg tttggcatcg ttgtccgcca cgatcaacaa
    19981 ctcgctcggt cccgccacca tatcaatgcc gacagtcccg tacaacatgc gtttcgccgt
    20041 ggcaacataa atgttccccg gcccgacgat cttgtccact tttaaaatgg ttttcgtgcc
    20101 gaacgccagc gcgccgatcg cctgcacccc gcccacgcga taaatctcgg tcactccggc
    20161 aatatccgct gcgacaagca aatagggatt gatgccgcct ttctgaggag gggtgcacat
    20221 gacgatccgc tttacccccg ccaccttggc tggaatggcg ctcatcagta cggtggaagg
    20281 atagacggct ttcccgcccg gcacgtatac gccgacggca tcaaggggag tcacgacctg
    20341 tcccaacgtc gccgctccat cctggtacat ccaggttttg gtccgttgcc gctcgtggaa
    20401 ggccatgatc cgttgggcgg caaagcgcaa ggagtcgcct tcctcttttc gtatcttgta
    20461 atagctttct ttgatttctt cgggagagac tttcagctga gtcgtggcga gcgacacgtt
    20521 gtcgaatcgc ttggtgaaac ggagaaccgc tttgtctccg ccccgctcca cggcgtccag
    20581 gatggccctc acgggttttt cggctgccgc cccttgaacc cgcgaacgcc ccgccacctt
    20641 tttcaacgtg gggaggaacg cccgatctgt ggaggcaaca atcttcatgt ccgcatccgc
    20701 ttaggctttc cagtcgcaga ccgtgccggc tttaccgatc cgtcttttcg agccggtcca
    20761 ggagtggcgc acgcatgccg gagtttgctg agcaactcgg cgacggcctg gtgtttcaat
    20821 ttgagactag cccgattgac gatgaggcgt gccgtcgatc gggcgatgca ctcgacttcg
    20881 atgaggccat gggctttgag cgtgttgcct gtttcaacca ggtcgacgat gcgatcggcc
    20941 agtccgacaa tcggcgccag ctcaatcgag ccatagagct tgatgatttc gaccggcacg
    21001 ccgcgctggt tgaaatagcg ctcggtaatc ttcgggtatt ttgtggcgac gcggattttt
    21061 gacgagagcc ggtcgcgtgg tacttgccct cgaaaagcgg cgacggcaat tctacacgct
    21121 ccgaacccta aatccaatgg ctcatagacg tcacagtcct gttcaagcaa cacatctttg
    21181 ccgacgacac ccacgtctgc cgctccgtgt tccacatagg tgggcacatc ggtcggccgc
    21241 acgatgagaa acgtcatccc gatctccgga caggggaaca acagccggcg gctatcagcc
    21301 gcgggctgcg ccgcgccata tcctgccttc gcaaacaact tgagcgttgg ctcgatcaat
    21361 ttccctttgc acaaggcaat cgtcagcatc cgctatcctt tttgtcggtc gatgacagcg
    21421 ccgagagccc taagcttctc ttcgattcgt tcgtagccac ggtcgagatg atagacgcgc
    21481 agcacgtccg tcgtcccttc ggccgccagg cccgcgagga tcaacccagc actggcacgc
    21541 aagtcggagg ccatgactgg agcgcctgca agacgttcgt gccccgtgat aacgacacga
    21601 ttgccctcga cgcgaatatc cgcccccata cggcgcagct cctctacatg catgaaacgg
    21661 ctttcaaaca cggtctccgt aatgatactg gtgccttccg ccaccgacat aagcgccacc
    21721 atctgcgcct gcatatctgt cgggaaaccg ggaaaaggca aggtccggac gtcgacaccc
    21781 ttcaatcgtc cccgctttga cgcgtcgata tggacgcgct ccttttcgac tcgaatggac
    21841 acgccggctt ccccgagctt gttcagcaac ggctccagat gctccggccg gcaccgattc
    21901 actgaaatcg atccttttgt aatggccccc gcaacgacat aggttccggc ctcgatgcgg
    21961 tccggaatga cctcatgatc gctgcctcgc agctcccgga cgccttcgat ggtgatgacg
    22021 tcggtgcctg ctccggctat tctggctccc cgcttgacga gaaagtttgc caaatcgacg
    22081 atttccggtt ccttcgcggc gttctcaatg acggtggtcc cctcagcgag tgaggccgcc
    22141 atcatcaggt tttcagtgcc cgtcaccgtc ggcacatcgc aatagatcgg ggcacctttc
    22201 agccgtttgg ctttggctcg aatgtacccg tgttcgatgg agacttcggc accgagcttg
    22261 gccaacccgg cgagatgcaa attcaccggc cgcgagccga tcgcacaacc gccgggcaag
    22321 gacacaacgg cttctcccca acgggcgacc agtggaccaa gcaccagcac ggacgcacgc
    22381 atggtcttga ccaattcata gggtgcctgt gtcgaccgaa tcacgtcggc cttgatgact
    22441 gcgcggttcc cttcatgatg cacgtccgca cccaagatcc cgagcagctt ccccatcgtg
    22501 accacgtcca cgacgcgcgg gatgttactg atcacgcact cgccgccacc gagaattgtc
    22561 gaggccagga tcggcagcgc agaattcttg gctccgctga cctggacctc tccctgaagt
    22621 acatgcccgc cggagatgac gattttgtcc atactgctgc cccgccctcc taccgtgccc
    22681 gcctgacgat caccacccgg ttgatgcctg cctcgtcggg caccacctcc acccgttcgt
    22741 aatccggcat cgccgcgatt tttgaacaga gctccaggct ctgcccctct cccacttcca
    22801 tggcaagcca tccgcccggc ataaggtaag aaactgcttc gcgtagcagc cgctcgtgca
    22861 cttctgttcc tcgtggacct gcaacaagtg ccagccgagg ctcatagcgg ctcacttccg
    22921 gttggagtcc agcccactcg gattcacgaa tgtagggagg atttgccagg atcgcatgca
    22981 tcgaaccttc caggcccaag ccccgcaacg gacccaacaa atctccatgc aaccaggtaa
    23041 tcgatgagtc gacgccgtga cgtcgggcat tgcgcatggc ggtctcgagc gcgtgcctgg
    23101 agatatcggt cgcgagaatc ctcgcccggg gaatcgaccg agccagtgtc acggccaaac
    23161 aaccggatcc cgtaccgaca tccatgagcg tgggaggcgt gccttgtggc agccgacgga
    23221 tgatctcgtg cgccagcaac tcagtttcag ggcgggggat gaggacggtt ggattgactt
    23281 caaactccag cccgcagaat tcctgggtcc ccagaatgta ttgcaggggc tctcgagcaa
    23341 cccttcgggt cgctacgtca tgtagtttcg ctatctccgg gtcagtaagc acgcgttctc
    23401 ggtcgacaat ttgatgtaat cccgtgaggg ccaatgcatg ctcgatcaac cacaaggctt
    23461 cctgctctgc agagtcgata ccggcagcct cgagccggcg gcgcatgtcc tggactgcgg
    23521 aaccaagcgt aggacggtgc acagctttgt gctgaaccga cggactcatg aggcctccgc
    23581 cgcagcgtga tcgcgttggg cgcgcaaggc ttcgaccaat tcgtccatat caccttccag
    23641 tacttgttcc aacttgtgca atgtcaatcc gatgcgatgg tctgtgacac ggttttgggg
    23701 gaaattatat gtgcggattt tttcgctgcg atcaccggtt ccgacctgag cctttcgggt
    23761 ttgcgcaatt tctgcctcct gtctttcccg ttctgcttcg acgattctgg cgcgaagcgt
    23821 ccgcatggct tttgtacggt tcttgagttg cgagcgctcg tcctggcagg acaccacgac
    23881 gccggtggga atatgcgtga tacgcaccgc ggagtatgtc gtatttacgc tctgaccgcc
    23941 ggcccctgat gaacaaaagg tgtcgatccg caggtccttc ggatcgatgt gcacgtccac
    24001 ttcatcgact tcgggcatga ccgcaaccgt gaccgtcgaa gtatggatcc ggcccgccgc
    24061 ctccgtcaca ggcactcgct gaacacgatg gactccgctt tcatacttga acaggccata
    24121 cccacccttt ccctcgatga cggcaatgac ggtcttgtat cctcccttgc cggtttccga
    24181 ggcctccacc atgtcgactt tcaacttgcg ccgttccgca aatttgacgt acatccggaa
    24241 cagatcaccg gcaaataagg ccgcctcatc accacccgtc ccggctcgaa tctccaaaaa
    24301 cagattttta tcatcccgag gatctttcgg tgtcagatgg tcaagcacct gtgcttcgag
    24361 cgcctcgcgc tccatctcca gctgggcagt ctcttcggct gcaagcgagt ggagttcggc
    24421 accagccgcg ggatccgacg acatatgggc ggcctcctcc aattgcctca gaacgtcctg
    24481 atatcgccgg aacagcaccg caaccccttc cagttccg
//
LOCUS       DASSCQ010000020        23805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_822440, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000020 DASSCQ010000000
VERSION     DASSCQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 23805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23805
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822440"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFH05_01355"
     CDS             complement(<1..377)
                     /locus_tag="VFH05_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676891.1"
                     /translation="MPLIWCAISGHGYGHAAQVVPVLNALGREIPHLSVLLRTTVSAS
                     FFQDRLTIPWTLQSVQQDVGCIQNGPLDIDIPSTWNALEEFHIGWEARVAVEAKAMTL
                     AAPALVLADTPYLAASAAKHAGIP"
     gene            complement(493..693)
                     /locus_tag="VFH05_01360"
     CDS             complement(493..693)
                     /locus_tag="VFH05_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676892.1"
                     /translation="MDLETAKQRIDSAITQYGQHAAPAIDLVMNEVRSDLGAKAFNDL
                     VEAFDLELLYNIAPLESDHSSS"
     gene            complement(705..1022)
                     /locus_tag="VFH05_01365"
     CDS             complement(705..1022)
                     /locus_tag="VFH05_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676893.1"
                     /translation="MDLDSFRQMVTKNPKGFLGRYGLGNKILQEGGSLEEAVEHLRVA
                     VQLDPLHVASHLALGRALVGLHRPNEAKPVLQAGIDAATSGRSNGGRDLVPEMEALLR
                     SLG"
     gene            complement(1101..1862)
                     /locus_tag="VFH05_01370"
     CDS             complement(1101..1862)
                     /locus_tag="VFH05_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676894.1"
                     /translation="MNPNTLRSLTLLCCAALSILTMVTGCSRWIEMAPRLDTQDPGAQ
                     QIARDEQVPLIIDVIRTTRNGSPQNTSAETQQRILSSLAEIGLFSQLSSGAYAVSPSN
                     DKIVRARILLSETIDPHAGGAAWKGIVIGASMYTLTPFLPLEYGYAANVSLELERWDG
                     QVKRYEGESSGTARYQLFGATPIMIEELKGHVTESCLNELARQIVRDTAFYTASSAPI
                     SHSDIRSVSIRRLSPKVPEASSAPIPVTVSAPADR"
     gene            complement(1872..2477)
                     /locus_tag="VFH05_01375"
     CDS             complement(1872..2477)
                     /locus_tag="VFH05_01375"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HET6676895.1"
                     /translation="MTPPSLAARIAFAALMFTMTIPCQAEEVRPHLTVGTQEISLSAG
                     YLLPHRLTTEHTTKQSGPALMPSWAMVLTDPIGKSFYRGQLLLGAEIVYIQFQEPFLT
                     HGVGFTPKIKYMFEGTGRLRPYIEFAGGPFYTDLGGRIPEESSRFNFILTAGGGLSWF
                     FTSQFSFNVGYRFHHISNAGTRMPNLGLNSSLPFGGFSFYF"
     gene            complement(2771..3559)
                     /locus_tag="VFH05_01380"
     CDS             complement(2771..3559)
                     /locus_tag="VFH05_01380"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676896.1"
                     /translation="MAESHKHRARIFLHRGDLVQARAAWEAAVLDDRQAGNPQELSNS
                     LGNLGNACALSQDYDAAARCYREVLDIQRTQHNTHAIAHTLVNLGNLHIGTGHPEQAR
                     PYYLEALDLLRTLNDHRGLGILYNNLALQEAREEQWTQALESFAQALSYHRMVGNEEG
                     LAVTYSQMGRCFLDQGDLVSAERCLNNGSEHYIKLGAEVGEAAVLRVLAEVYNRRGDF
                     VSAVRCLERTVLIATTYALPDLSRDSQELDRLKGLATQLHPGPE"
     gene            complement(3575..3997)
                     /locus_tag="VFH05_01385"
     CDS             complement(3575..3997)
                     /locus_tag="VFH05_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676897.1"
                     /translation="MQFDAALAAQTAFHHAEDELGADWETAVALEEAFSSQAGHAARD
                     AYDALLNLATRHPHAHCFHAFCIYITWQQVTEETLACHFHTGVRLSEHFLASIDGKRP
                     ADVEQIRELRESFRAGLGMEEEDDLQVEYRRDTPKGGD"
     gene            4117..5838
                     /locus_tag="VFH05_01390"
     CDS             4117..5838
                     /locus_tag="VFH05_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET6676898.1"
                     /translation="MEGNRSAEERTDPVDQIIRYHLQTKHHFNRYARALGYLDWANQP
                     DPFRRYDGTQLTPLPLLSPEHVPVSPSYSAIFVGDGVLPHPVTVDSLSRFFEFALALS
                     AWKRAGESEWALRSNPSSGNLHPTEGYVVLPSLEGLGARPGLYHYAPKEHALELRAEM
                     SGDALASLLRPFPARAFVFGLTSVHWREAWKYGERAFRYCHHDVGHAIGTARIAASTL
                     GWQAVLLDGCDQNTVATLLGTHRAEDFAQGEPEHPDCLLAVWPRVPDAHHAPVPLFLD
                     PTAVKEVVQTTWRGNANRLSHDHGVHWDIIDTAAESTWKTQTNEVTIELAPARTSFDG
                     TASDGPVSAGQMIRQRRSAVAFDAATSIAAATFFHMLRRVMPRVELAQSARPMPWDCW
                     PYSPAVHLLLFVHRIDGLAPGLYFLARDPNKVAFLQGAMNAELTWQLAPGCPEDLPLY
                     WLLEGDAKKLAVQVSCHQDIAGDSAFSLGMLAEFEGLLRERGAWWYPRLFWESGLLGQ
                     VLYLEAEAAGVRATGIGCFFDDPVHEIVAVKGLSLQSLYHFTIGGPVEDRRLMTLPPY
                     RHLKRHA"
     gene            5861..6721
                     /locus_tag="VFH05_01395"
     CDS             5861..6721
                     /locus_tag="VFH05_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676899.1"
                     /translation="MFKIKKKTDKPKATVLYNIVEDYSEFDAPGNVTVCAMTLMEDLP
                     THARVLSESYDVPFESMSQLLKEGGTYVYPHQSGLLTRGLFACLLGDADGTPKQTFVL
                     DVMQFAEAEQAARARSVSLAVAAKDVLYRTLPEELKLKLAQKNLGLDYRTLDRAVAKG
                     GDVKYVDFRKDWSPHFKRLCIMPDGRLVETGGLDEFARLHGISPAQAKVLVEQGGTLE
                     VGEEVLACQIVNGQPAVARFTAKNYVKAKELAAAKSLHIMDALSEVAYQDPAMMRSLV
                     RKELTGGRQT"
     gene            6771..7157
                     /locus_tag="VFH05_01400"
     CDS             6771..7157
                     /locus_tag="VFH05_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HET6676900.1"
                     /translation="MGIFLASCAPKPILYPNQHYKDAGRDAADQAIEECSQMAKDAGA
                     TPSRGKTGQVAGSTAGGGAVGSAAGAVGGAVVGHAGRGAMVGAASGATAGFLRGLFRR
                     SPPSQAYKEFVQRCLKERGFDPVGWE"
     gene            complement(7167..7529)
                     /locus_tag="VFH05_01405"
     CDS             complement(7167..7529)
                     /locus_tag="VFH05_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6676901.1"
                     /translation="MATILVIDDEDSIRHLLKDVLEKAKHRVLEARDGREGLNLYQNN
                     QIDLVLMDILMPGTDGLEATLQLTREYLDAKIIAMTGAQGDRNFLDVAKLFGARRVFE
                     KPFDLTKMVEAVNEELAK"
     gene            7892..8377
                     /locus_tag="VFH05_01410"
     CDS             7892..8377
                     /locus_tag="VFH05_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HET6676902.1"
                     /translation="MSPTPIRAEAGALPEGELSFYNVHTDERLRVRYRDESGRYDLSA
                     LDDLNHILRCHHTGEVAAMDPRVIEHLNLVQKTLGGEGEIHVISGYRSPEYNAILVKR
                     SRRAARHSLHVEGQAVDFFIPGVRPREIKQAALKHRYGGVGYYPRAQFIHLDCGPFRS
                     W"
     gene            complement(8403..10610)
                     /gene="glgB"
                     /locus_tag="VFH05_01415"
     CDS             complement(8403..10610)
                     /gene="glgB"
                     /locus_tag="VFH05_01415"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET6676903.1"
                     /translation="MSALSRQHIEQLVTGSHWDPLAILGPHMSQQNGVPALVIRSFLP
                     EAREAVVLAQTSDEVAFPMARIHESGLFEAVLPPAPAPSHYRLRVVDHAGRLSERYDP
                     YAFAPLLSDFDLHLFAEGTFFKAYETLGSHVRRIDSIPGIHFVVWAPNAGRVSVVGDF
                     NQWDGRRHPMTNRGATGLWELFIPGLEDGTIYKYEIRPRGRDGFLLKADPYAVASELR
                     PKTASIARDISAYRWNDQAWMENRRHQNPLEVPVTVYEVHLGSWMRIPEEHNRWLTYR
                     ELAAKLIPYVKDMGYTHVELMPVTEHPFDASWGYQATGYFAVTSRYGTPEDFMAFVDA
                     AHQAGIGVLMDWAPAHFPDDAHGLSQFDGTHLYDHADPRLGYHPDWHSRIFNYGRVEV
                     RNFLLNSALSWLDRYHIDGLRVDAVASMLYRDYGRKSGEWIPNQFGGHENLDAVLFLK
                     DLNVLVHRDFPGAMMLAEESTSWAGVSRPTYTGGLGFTFKWNMGWMHDMLDYFSHDPV
                     HRIYHQNQLTFGLLYAFNENFMLPLSHDEVVHGKGSLINKMPGDEWQQFANLRALYGY
                     MYGHPGKKMLFMGGEFAQRDEWTHDHSLDWHLLQYGYHQGVQNLVRDLNRLYRSERAL
                     HAVDHDWQGFQWVDFHDAANSIVVFLRKSHDPDEQILCVCNFTPVPRYDYRIGVPHER
                     FYRELLNTDASLYGGSNMGNGGGVHAHRDPSHGFPCSLQLTLPPLSVTYLKAG"
     gene            10767..12107
                     /locus_tag="VFH05_01420"
     CDS             10767..12107
                     /locus_tag="VFH05_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET6676904.1"
                     /translation="MHPSELSIALFIFGLAYAVIITERIHKTIVAVSGAALMIASGVV
                     TQDEAFYSHEFGVDYNVVFLLIGMMVIVNIVRETGLFEVLAIWSAQRSGAKPLRLLMM
                     LAIVTAALSAMLDNVTTVLLMAPVTLAITKRLSLNPVPYLIIEAVASNIGGTATLVGD
                     PPNIMIASKADLGYVDFLVVLGPIVTVIMAVFLGCLKLFFGTMQVAPHQANAVMQLDA
                     RDAVQDPVLLRRCVWLLALINVAFCIHGALHLEPATIALVGASAFMLIGHATKKTDGD
                     ELSYLTDVEWKTIFFFIGLFILVGGLVKVGVIRFLAGQLVSFTQGHPAATTFSVLWGS
                     AILSAIVDNIPYVAAMNPLIVDLARSAHPDVTDYTTLVHQPDILPLWWALALGACLGG
                     NGTIIGASANVVIVDLARKAGYPISYWDFFKLGFPVMVGSVLLSTLYVWLVFLR"
     gene            complement(12483..12770)
                     /locus_tag="VFH05_01425"
     CDS             complement(12483..12770)
                     /locus_tag="VFH05_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676905.1"
                     /translation="MLLERRSRFGRIMWCGIGLVCLCLVMYMLGSTMTLWAMEFPLDQ
                     ADSPLLEGFSLPTTLSDLVPLLLASSTGESSTTVLRPVSEHGLLRPPIPTA"
     gene            complement(12840..15116)
                     /locus_tag="VFH05_01430"
     CDS             complement(12840..15116)
                     /locus_tag="VFH05_01430"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET6676906.1"
                     /translation="MCLAVVVMFAVFGVRTHAEVQINTSDQPSQKASEAASKQPPDDA
                     SSLSELRAKRDQAQRELDAVSRPNQLSTGAPPGTPEPELLERRTLLHHIVRSADEQID
                     ATLRLEQARQHRDEVGRSLPDSKDHGDTPATIFDADRLWDTAFALRLAVEGLQSQLAL
                     IELRSTRAKDALQAAEERLRQASEHLESTTSPSGADRNRWLRDLEALRQRAAAALLHA
                     TELSKIRIGDELSDARARLQAAESRLSAADQLVEFTEPDLEKIRTRLSQERQALTHEL
                     EETTTARHGQFEALRDLERQLASRMAKGEGKAGKKPSESLRRIQAGTEVGRIRADNLA
                     LKSDLWQLLLDVVDSERQLWESRFAIMHNPEPGKAREAYDRFTPLFTNLQASRDYVRQ
                     QLSIVSGQIGELETRVTNSRPGQDRNHVRALLEAHRQRETIYNYGLQRLDQTSRFLER
                     WKSAFKGQQKELPMTARVEEWWQHAWDRTNAIWHVEVFNAEDTIEVDGKKITGHRSVT
                     VGKIVTALIILTIGYWLCLYLAAQIGRLAVKRLAMAPEVANLLRQWSQALLLTILIVT
                     SLVWVKIPLTIFAFLGGAFAIGVGFGAQNLLKNVISGILLLIERPLRVGDLIEVENVR
                     GRVTTIGLRSSTVRDAKGVVTLIPNSSFLERHLTNWTYSSRIGRFSLRVGAPYDSSTQ
                     LVMDLLAGLAASHRQVLKTPKPQVLLDEFGNQGRIFTLNYWLEIRLDVDPNEVASELR
                     FGIEQKFAEAGVKILPAA"
     gene            15372..16481
                     /locus_tag="VFH05_01435"
     CDS             15372..16481
                     /locus_tag="VFH05_01435"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6676907.1"
                     /translation="MTVRSHVARQQNWLMTVRLKPIKEQVIVITGASSGIGLVTARMA
                     AKRGARVMLTARNHDALKQLADEIVANESNGGMAAFLAADVADEAALRRVADEAVSRF
                     GRIDTWVNGAGVSVYGRLTDVSLEDQRRVFETNFWGVVIGSRIAIPHLRTGGALINIG
                     SVLSDRAVPLQGVYCAAKHAVKGFTDSLRMELEHDDLPIAVTLIKPYSIDTPYPEHAK
                     NYLPTEPTLPFPVYAPEVVAETILHCAEHPTRDVYAGGGAKIMAAMGGLAPRLTDRLM
                     ENLIDMQLTDRPEDDRTNNSLYGPTTGLKERGGRAAYVAESSLYTQVSLHPLLTGAAL
                     AATGLTLASWLFRRTSAAKYEPTGHHWYEADRVKH"
     gene            16505..17038
                     /locus_tag="VFH05_01440"
     CDS             16505..17038
                     /locus_tag="VFH05_01440"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET6676908.1"
                     /translation="MQTITGWLLLATAAAFLPATGGQEAKRDTLSTAQSFLMKAAEGQ
                     QAEIEMGRLASQRGGAEQVKQFGAQMMEDHQKASKEIQLLASKEGVVLPTELTGKHKD
                     KREEFGQLSGDAFDRAYIRYMLRDHQKDVKEFERHAKAIKHPPVQRWAEGTLPLLKQH
                     LQTAQRIASSIGVEGVR"
     gene            17075..18538
                     /locus_tag="VFH05_01445"
     CDS             17075..18538
                     /locus_tag="VFH05_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676909.1"
                     /translation="MKIKQVGLNRVLIVAGAIVAVSMIVSQAERTGLAQPTPEGKKQQ
                     AQMGTIEETKQKGFEGVDFSYDVFGAPPGQDPQKIADEVMKKDIAQKPDVMKKQHKLL
                     SERYDLNCRTEKGIAMSRGKPQPIGPAVKLASGMTWDQLAQLSPEDIKKKGVFPQGFD
                     RLPHVKHAVGGQVFPQVQIKEFPRLERFDVDFDLPECLLPEFPPPIFLTTHPELGDVS
                     QGEVVHAENFDRLFRGLVTPAQLDGLRMLVTQFAQEEFNATHDRKTAKPSLGVSCLDC
                     HVNFHTTGQFHLNPDTRPQMERLRLDTVSLRGMFNQQIHGSKRSIRSVEDFTEFEQRT
                     AYFNGDPIRAMKKGMNIIDRVPVAHMAQMQNMIDFPPAPKLDPLTGRLDRTKATPLEI
                     KGEDLFFGKAKCAACHRPPAFTDHHMHDLHLERFGAAADGPIKTFVLRGIKDSPPYFH
                     DGRLPTLEDVVEFFNVIGGLKLDQDEKKALVAFMRAL"
     gene            18566..18808
                     /locus_tag="VFH05_01450"
     CDS             18566..18808
                     /locus_tag="VFH05_01450"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HET6676910.1"
                     /translation="MEDQMDGTVLRRIGELVSLEHSFQEQELLSDDDRARLRGVQVEL
                     DQSWDLLRQRRALRAAGQDPNKAQVRPAGIVQKYVQ"
     gene            18845..19294
                     /locus_tag="VFH05_01455"
     CDS             18845..19294
                     /locus_tag="VFH05_01455"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6676911.1"
                     /translation="MSVIEKSIEINVPVRAAYNQWTQFEEFPKFMEGVKQVKQLDDKH
                     LHWKADIGGKEKEWKAEITEQIPEERIAWTSRGGAINAGVVTFHRRSDSKSKVMLQME
                     YDPQGFVENVGDAVGVVTQRVQGDLERFKQYIENRGQETGAWRGTVK"
     gene            19300..19824
                     /locus_tag="VFH05_01460"
     CDS             19300..19824
                     /locus_tag="VFH05_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676912.1"
                     /translation="MARIVFRQLQRSAVPDSNGRFWPRTIAAGCLSGMTVAGAAAWRA
                     QMEGQSGWGPINAISHIVWGPQAAAQQRFTAKYTGSGLLLNALACGFWGWLYGVMGRY
                     RPAPHSYSRSAGRGIVVAALAYITDYYLVPRRFTPGFELSLTRRSFPWLYGALAVGLI
                     LPDCLVPPKSRSRS"
     gene            19821..20330
                     /locus_tag="VFH05_01465"
     CDS             19821..20330
                     /locus_tag="VFH05_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676913.1"
                     /translation="MNGQRLVTYMCACMLLQAVPVFCQDAILPQGDPRLRDDPQEAKQ
                     REEIRDRRMGQQGQGPDYRVEGKPQGGTSGATAGEANGRTRQDTGLSDPSVNPGQASG
                     MRVLRGEVKQANPKSIVIEDRHGKETTMSVDAATAGDRDIRPGDFITGTVTTQGRAVA
                     IHKESAPGR"
     gene            20417..21484
                     /locus_tag="VFH05_01470"
     CDS             20417..21484
                     /locus_tag="VFH05_01470"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6676914.1"
                     /translation="MTTRSLQGTSPKTTRPGKSSSKTQEGSPATGQQLSTVSDRLREN
                     GNGNGSLTQEEQLAYGLGWFGIGLGLTELMMPRRLAHATGLPVRHRGLIRIMGLREIA
                     SGLGIITQRRPAGAVWSRVAGDVIDLACLGVAFTSAGADRKKLAAAVTAVAGATALDL
                     LCAQQLTRDVVTRNGTIAVTVSLIIDRSPGDLYSYWHDFTNFPSFMKHVISVQVTGER
                     RSHWVVTGPAGSTVEWDAEVTEDSADTLIAWRTLEGSDVDHRGQVRFEPAPGKRGTLV
                     TVDLQYRPPAGTLGSAIATWFGQNPEHSMKMDLRRFKQVMETGEVITTEGQPAGRQGS
                     TSWKYDRAVRGSGTPRTRGRT"
     gene            21539..22705
                     /locus_tag="VFH05_01475"
     CDS             21539..22705
                     /locus_tag="VFH05_01475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET6676915.1"
                     /translation="MKANCWYGKRHVQVVDVPDPIILNQRDAIVKITSTAICGSDLHL
                     YNGFVPTMEQGDIMGHEFMGEVVEVGREVKKLRVGDRVVVAFPISCGNCFFCKGEMYS
                     LCENSNPNAWMAEQFFGHSPAGIFGYSHLTGGFAGGQAEYVRVPFADVGTVKIENGLS
                     DEQVLFLSDIFPTGYMAAENCNISPGDTVAIWGCGPVGQFAIRSALMLGAERVVAIDR
                     VPERLKMARDGGAHTIDYEETDVYDMLMQFTGGRGPDSCIDAVGLEAHAPTPLYYYDR
                     LKQAMMLESDRPIALREAIMACRNGGTVSIPGVYGGLIDKMPMGAVMNKALTLKTGQT
                     HTQRYLKPLLKRIKDGDIDPSFVVTHRLKLADAPQGFATFNDKQDGCIKIVMTP"
     gene            22729..23595
                     /locus_tag="VFH05_01480"
     CDS             22729..23595
                     /locus_tag="VFH05_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6676916.1"
                     /translation="MKPVWSTSLLAGMILWCAGDVLALAEVKSDASMEALRWAKQAEA
                     SGVARDQARFVEEIDRALQVALDAGAKTPNHRLAEAVHALMEARALGQQGNLAEGRED
                     LQSAIVWLTRAAGLPAANPSADWSMNQRDSTLLKGQSARPGKEHPMDHFLIDIEEATI
                     RNNNFRQVLFTASHSQLVLMSLKPGEEIGTETHHLDQFIRVEAGEGSARLNGKDYALK
                     DGSALVIPAGTEHNVINDRKDQPLKLYTIYSPPEHKDGVVHRTKEEAEADRDDHFDGK
                     TTASLHESPASR"
BASE COUNT         5051 a         6535 c         7253 g         4966 t
ORIGIN      
        1 ggaatccccg catgcttggc ggcagaagcg gccagatacg gcgtatcggc gagcacgaga
       61 gcgggagctg ccaatgtcat ggccttcgct tcgacggcga cacgcgcctc ccagccgatg
      121 tggaattctt ccaaggcgtt ccaagtggag gggatgtcga tgtctaatgg tccgttctga
      181 atacagccaa cgtcttgctg aacggattgc agagtccagg ggatcgtcag ccgatcctga
      241 aaaaacgagg cggatacggt tgtccggagc agtacggaca ggtggggaat ctctcgtccc
      301 agcgcgttga gaacagggac gacctgggca gcgtgtccgt agccatggcc ggagattgcg
      361 caccaaatca gcggcatcag ggatcgttca gttctttcct gtttggactt ggaatgctct
      421 tggggcgaag cggttcagtc tgtgatggct tgttcgaaaa cgctgtgcag agacggcctg
      481 ctgctcacgc acctagctac tcgagtgatc cgattcgagg ggagcaatgt tatagagaag
      541 ctcgaggtcg aacgcttcaa ccaggtcatt gaaggccttt gctccaagat ccgaccggac
      601 ctcattcatg accaagtcaa tggcaggcgc ggcatgttgt ccgtactgcg tgatagctga
      661 atcgatgcgc tgtttcgcag tttcgagatc catatcatca cccgtcagcc tagggaacgc
      721 agtaaggctt ccatctctgg caccagatcg cggccgccat tggatcgccc ggatgtggcc
      781 gcgtcgatcc ctgcctgcag cactggtttc gcctcgttcg gccgatgtaa gccgaccagc
      841 gctcgtccca gcgctaagtg cgaggcgaca tggagcggat caagctggac ggcgacgcgg
      901 agatgttcca ccgcttcctc caggcttcca ccttcctgca aaattttgtt gccgagaccg
      961 taccgtccca ggaagccctt gggattcttc gtcaccattt ggcgaaatga gtcgaggtcc
     1021 atggcagcct ccggtagggg catcatacca tcggcgcttt ccgatgggcc agacgaaaat
     1081 ccgtcgcgag gactgcggaa ttaccgatcc gcgggtgcag aaaccgtaac gggaatgggg
     1141 gcagaggagg cttcgggaac tttggggctg agccgtctga tcgagaccga gcgaatgtcg
     1201 gagtgcgaaa tgggggcact gcttgcggtg tagaaggctg tatcacgaac gatctgtctg
     1261 gccaattcat tcaggcagct ttccgtcaca tggcccttga gttcctcgat catgatcggg
     1321 gtggcgccga agagctgata gcgggccgtg ccggaggact caccttcgta ccgtttgacc
     1381 tgcccgtccc accgctccaa ttcgagcgag acgttcgccg catatccgta ttccagcgga
     1441 aggaagggag tcaaggtata catggatgcg ccaatgacga tgcctttcca ggctgcgccg
     1501 cccgcatggg gatcgatcgt ttcgctcagg aggattctgg ctctcacgat tttatcgttc
     1561 gagggggata cggcgtacgc gcccgaggac aactgtgaga agaggccgat ttcagccagc
     1621 gaactgagga tccgttgctg cgtctcggcg gacgtattct gaggagaacc gtttctcgtc
     1681 gtgcggatca cgtcaatgat gagcggcacc tgttcgtcgc gagcgatctg ttgagcgcct
     1741 gggtcctgcg tgtccaagcg aggcgccatt tcgatccaac gactgcagcc cgtgaccatt
     1801 gtcagaatgg agagggcggc acagcagagc agcgtcaggg aacgaagtgt attagggttc
     1861 acggtatgct cctagaaata gaatgagaag cctccgaacg gcagactgga gttgagcccc
     1921 aggttgggca ttcgggtgcc ggcgttggaa atgtgatgaa atcggtagcc gacattgaac
     1981 gagaattggg acgtgaagaa ccaggagagg ccgccgcccg cggtgagaat gaagttgaag
     2041 cgcgaagact cttccggaat acgccccccg agatcggtgt aaaacggacc accggcaaat
     2101 tcgatgtagg gtctgaggcg acccgtccct tcgaacatat acttgatttt cggggtaaag
     2161 ccgacgccgt gggtgaggaa gggctcttga aactggatgt agacgatttc ggctccaagc
     2221 aacagttgtc cgcgatagaa gctcttgccg atgggatccg tcaagaccat tgcccacgat
     2281 ggcatcaagg ccggaccgct ttgcttcgtg gtgtgctcgg tcgtcaagcg gtgaggcaat
     2341 aaatagccgg cgctcaagct gatctcctgt gtgcccaccg tcaaatgagg acggacttct
     2401 tctgcctgac acggaatcgt catggtgaac atgagagctg cgaaggcgat acgggctgcc
     2461 aacgaaggag gcgtcattca gcatccggtt gaacctttgt cggctggtct gtacgccaac
     2521 aggaggtgcg caatcgtgac gcttccaaag gtaacagaga atgaaccggt gtccagtaag
     2581 agaggaaatt gtgcagagaa gatgtcaaac aagacgtatg aagagcatct tagggagtac
     2641 ccctattgta actgctttct cacagacaat gtcagattcc ggttaaggtg ggatgatgaa
     2701 cgtgttaatt tcgtgtccgc ggcgacgcag gtccatacga atggcgctgc gcgaagtgtt
     2761 gatcgcttga tcattcgggg ccagggtgga gctgagtcgc aaggcctttc agccgatcga
     2821 gttcctgcga atctcgagac agatccggaa gcgcatacgt tgttgcaatg agtaccgtcc
     2881 gttcgaggca acggacggcg gaaacgaaat cgccgcgccg gttgtagact tccgccaaga
     2941 cacgcaggac cgcagcttct ccgacttccg cgcccagctt aatataatgt tcggacccgt
     3001 tgttcaagca ccgctcggca ctcaccagat ctccctggtc gagaaagcat cgtcccatct
     3061 ggctgtaggt gacggccagg ccttcttcgt tgccgaccat gcggtggtag ctgagtgcct
     3121 gagcaaacga ctcgagagct tgcgtccact gctcttccct ggcctcctgc agcgcgagat
     3181 tgttgtatag gatgccgagg ccccggtggt cgttcaaggt gcgaagcaag tcgagggcct
     3241 ccagatagta cggccgcgcc tgttcgggat gccccgttcc gatgtgcaga ttcccgaggt
     3301 tgaccagcgt gtgcgctatc gcatgagtgt tgtgttgcgt tcgctggata tcgagcactt
     3361 cgcggtaaca tcgcgctgcg gcatcgtagt cttggctgag cgcgcaggcg ttgccgagat
     3421 tgccgagcga attcgagagt tcctgcggat tgcccgcctg gcgatcgtcc agcaccgccg
     3481 cctcccaggc cgctctggcc tgtacgagat cccctcgatg gagaaaaatt ctcgcgcggt
     3541 gcttgtgact ctccgccatg tccgctcggt ggtgctaatc gccgcccttg ggcgtatcgc
     3601 gtctgtattc cacttgcaaa tcgtcctctt cctccattcc caaccccgcc cggaacgact
     3661 cccgcaactc tcggatctgt tcgacgtccg caggtctttt cccgtcgatt gaggcgagaa
     3721 agtgttcaga cagccggaca ccggtgtgaa agtggcacgc gagggtttct tccgtgacct
     3781 gctgccacgt aatatagata caaaacgcgt gaaagcagtg ggcgtgagga tggcgcgttg
     3841 caagattcaa gagggcgtca tacgcgtctc tggcggcgtg gccggcctgg gaggaaaagg
     3901 cctcttccag tgccacggcg gtctcccagt ccgctccgag ttcgtcctct gcatggtgaa
     3961 atgcggtctg ggcggctagc gcggcgtcga attgcatgag acactcctac cgtcggacgg
     4021 caatgagtcg agtgtaacgt tgcgcgagcg ccatccgcaa ctcaacgcgc ttcgtttact
     4081 tttctccagg tggctttgta cactgggcgg tacggtatgg aaggcaatcg gtcagcagaa
     4141 gagaggaccg atcccgtcga tcaaatcatc cggtatcatc tccaaaccaa gcatcatttc
     4201 aatcggtatg cgcgcgcatt gggctacctg gattgggcga accagcctga tcccttccgt
     4261 cggtatgacg gaacgcaatt gacgcccttg ccgctgctca gcccggagca tgttcccgtc
     4321 tcgccgtcct attccgccat cttcgtaggg gacggtgtgt tgcctcatcc ggtgacggtg
     4381 gacagtcttt cgagattctt tgagtttgca ttggccctct ccgcttggaa gcgggcaggg
     4441 gaatcggaat gggcattgcg cagtaaccct tcatccggaa atctccatcc gaccgaagga
     4501 tatgttgtac tgccctctct cgaggggttg ggtgcccggc caggcttata ccactatgcg
     4561 ccgaaggaac atgcgctgga gttgcgggcg gagatgtcgg gcgacgcgtt ggcgtcattg
     4621 cttcgtccgt ttcctgctcg tgcctttgta tttggactga catccgtaca ctggcgagag
     4681 gcttggaagt acggcgaacg ggcattccgg tattgtcacc atgatgtggg acacgccatt
     4741 ggcacggcgc gcattgccgc ttcgaccttg ggatggcagg cagtgctatt ggacgggtgc
     4801 gatcaaaata ccgtggcgac gttgctggga acacaccggg cagaagattt cgcacagggg
     4861 gagcctgagc atcccgattg tctcttggcc gtctggccaa gagttccgga tgcccatcac
     4921 gcccctgtcc ccctctttct tgatccaacc gcggtcaagg aggtggtgca gactacatgg
     4981 cgcgggaacg cgaaccggtt aagccatgat catggcgtgc actgggacat tatcgatacg
     5041 gcggccgagt cgacgtggaa gacgcagacg aacgaggtga cgatcgagct tgcgcctgcc
     5101 cggacgtcat tcgacgggac tgcgtcggat ggaccggtat ccgcaggaca gatgatccgg
     5161 cagcggcgca gcgccgtcgc attcgatgcc gcgacctcca ttgccgctgc aacgtttttc
     5221 cacatgcttc gtcgggtcat gccccgtgtg gaattggcgc aatcggctcg cccgatgccg
     5281 tgggattgct ggccgtacag ccccgcggtt catctcctgc tcttcgtcca tcgcatcgat
     5341 ggcctcgcgc ctggactgta ttttctcgcc cgtgacccca acaaagtggc ctttctccaa
     5401 ggggcgatga atgcagaact cacgtggcaa ttggcgcctg gatgtccgga ggacctcccg
     5461 ctgtattggc tgttggaagg cgatgccaag aaactcgcgg tccaagtaag ttgtcatcag
     5521 gacattgcgg gggacagcgc attttcactg ggaatgctgg ctgagtttga gggactgttg
     5581 cgggaacgag gcgcgtggtg gtatccacga ctgttttggg aatcagggct cctgggacaa
     5641 gtgctgtatc tcgaagcgga agcagcgggg gtccgggcca cagggatcgg gtgttttttt
     5701 gacgatcccg tccatgagat tgtcgctgtg aaggggttga gccttcaatc actctatcac
     5761 ttcaccatcg gaggtcctgt cgaagatcgt cgactgatga ccttgccgcc gtatcgacat
     5821 ctgaaacggc acgcgtgaag cgagcgaacc ggagcgagcc atgttcaaaa tcaagaagaa
     5881 gacggataag ccgaaagcca cggtgctgta caacatcgtg gaagattatt cggagtttga
     5941 tgcgccgggc aatgtgaccg tctgtgccat gaccctgatg gaagacctgc cgacgcatgc
     6001 gagagtgctt tcagagagct acgatgtgcc ttttgagagc atgagtcagt tgttgaaaga
     6061 gggtgggacc tacgtctatc cccatcagag cggcctcctc acccgggggc tattcgcctg
     6121 cctcctgggc gatgctgacg gcacgccgaa gcagacgttc gtcctggatg tgatgcaatt
     6181 cgccgaagcc gaacaggcgg cacgcgcccg gtcggtttcc ctggcggtgg cggccaagga
     6241 cgtgttgtat cggacgttgc ctgaggaatt gaagctgaag ctggcccaaa aaaacttagg
     6301 ccttgattac cgcacgctgg accgagcggt tgccaaggga ggcgatgtga aatacgtcga
     6361 ctttcgaaag gattggtcgc ctcacttcaa gcggctgtgc atcatgccgg atggccgtct
     6421 cgtcgaaacc ggcggattgg atgaattcgc ccgcctgcac ggcatttccc ctgcgcaggc
     6481 gaaagtgctg gtggaacagg gagggacgct ggaggtgggt gaagaagtgc tggcatgtca
     6541 gatcgtcaat ggccagcccg ctgttgcccg gttcactgcg aagaattacg taaaagcgaa
     6601 ggagctggcc gctgcaaaaa gcctccatat aatggatgcg ctgagtgagg tcgcctatca
     6661 ggatccggcc atgatgcgga gccttgtgcg caaggaactg actggcggcc ggcagacgtg
     6721 accggaagcg ttgcgggatg atgagcagga tcgtcagcag gtggctcctt atgggcatct
     6781 tcctcgcgtc ctgcgccccg aaaccgattc tgtatcccaa ccagcactat aaggacgcag
     6841 gtcgagacgc tgcagaccag gcgatcgaag aatgcagcca gatggccaag gacgcgggag
     6901 cgaccccaag ccggggaaaa accgggcaag tcgccgggag cacggcgggg ggtggagcag
     6961 tggggtcagc ggctggtgcg gttggaggag cagtcgtcgg acatgccggc cgcggagcca
     7021 tggtcggagc cgcaagtggc gcgacggcgg gttttcttcg agggctgttc aggcggtcgc
     7081 ctcccagcca ggcgtacaag gagttcgtgc aacgatgttt gaaagaacgt ggtttcgacc
     7141 cggtcgggtg ggagtaaacg aacgggttat ttggccaatt cttcgttgac cgcttcgacc
     7201 atcttggtca gatcgaaagg cttttcgaag acacggcgag ccccgaacag tttggccacg
     7261 tcgaggaaat tgcggtcgcc ctgcgcaccg gtcatcgcga tgattttcgc gtccaagtat
     7321 tcgcgtgtca gttgaagggt cgcttccaac ccgtcggtgc ccggcatcaa aatatccatg
     7381 agcacgagat cgatctggtt attctggtaa aggttcaatc cttcgcgtcc gtcgcgcgct
     7441 tcaaggaccc gatgtttggc cttctccagc acgtccttta ataagtgccg gatcgaatcc
     7501 tcatcatcga tcaccaaaat ggttgccatg cccatggctc cttgtaagtg acgtgatatt
     7561 actcgtcttc ctttgacctg tctagcgaga aatgtctctc gcgaagattg ctcgctcatg
     7621 ttctccgcgt gtgaatgtcc acgagttaga gtcccttagg gaagtctaca ggccttgtgg
     7681 gactccctca ccgacgcggt ccatatgctc gcaagagaaa aacttggttg acctgggtcg
     7741 gcctatttga tacaagcctc cgactacgta cgtatcctcg ctgggctttc tttcctggag
     7801 cgatgctgtg ccagcctcag attggttgtg gacaagacga accgttttga aagcttcagt
     7861 ggccagtgtt tttctctgga gcgcacgggt gatgtcgccc acaccgattc gcgcagaggc
     7921 cggtgcctta cccgaaggcg aactgtcctt ctataacgtg cacaccgatg agcggttgcg
     7981 ggttcggtat cgggatgaaa gcggacgcta cgatctttcg gcgctggacg acctgaacca
     8041 catcctccgc tgtcatcata ccggcgaagt ggccgcgatg gatcctcgtg tcattgagca
     8101 cctcaatctc gtgcagaaaa cgcttggcgg ggaaggcgag attcacgtca tttccggcta
     8161 tcgttctccg gaatacaatg ccatcttggt gaaacgaagc cgtcgggccg cccggcatag
     8221 tttgcatgtc gagggccagg ccgtggactt tttcatcccc ggcgtacggc cgcgtgagat
     8281 caaacaggct gccttaaaac accgatatgg cggggtaggg tattatcctc gcgcacaatt
     8341 tatccatctg gactgcggtc ctttccgttc ctggtaattc tcttcactct tcccccaatc
     8401 cttcagccgg ctttgagata ggtcaccgac aacggtggca atgtgagttg cagtgaacaa
     8461 gggaagccgt ggcttgggtc cctgtgcgcg tggacgccgc ctccattgcc catgttgctt
     8521 ccaccgtaca gggaggcatc ggtgttgagc agttcccgat agaaccgttc atgaggcacg
     8581 ccgattcgat agtcatatcg cggaacagga gtgaagttgc acacgcagag gatctgctca
     8641 tccgggtcgt gggatttcct caaaaacacg acgatggagt tcgccgcgtc atgaaaatcg
     8701 acccactgga atccctgcca atcatggtcg acggcatgga gggcgcgctc ggaccggtac
     8761 aggcggttga ggtcccgtac cagattttgc actccttggt ggtagccgta ctgaagcagg
     8821 tgccagtcga ggctgtggtc gtgagtccat tcatcgcgtt gcgcgaattc gccgcccatg
     8881 aacaacattt ttttccccgg atggccgtac atgtacccgt acagggcgcg caaattggca
     8941 aactgctgcc attcgtcacc gggcatttta ttgatcagcg accctttgcc gtgcacgact
     9001 tcgtcatgcg aaagcgggag catgaagttt tcgttgaagg catacagcaa tccgaacgtt
     9061 aactggtttt gatgatagat ccgatgcact ggatcgtgac tgaaatagtc cagcatgtcg
     9121 tgcatccagc ccatgttcca cttgaaggtg aatccgaggc cgccggtata ggtcggccgc
     9181 gagacgccgg cccaggacgt ggattcctcg gccagcatca tggcacccgg gaagtcacgg
     9241 tggacaagga cattcaaatc tttcaaaaag agcacggcgt cgagattttc atgtccgccg
     9301 aactgattgg gaatccactc gcccgatttg cggccgtaat cccgatagag catggaggcg
     9361 actgcgtcca ctcgaaggcc gtcgatgtga tagcggtcta accaggagag tgcgctgttc
     9421 aacagaaaat tccgcacctc aacacgtcca taattgaaaa tgcggctatg ccagtccgga
     9481 tggtagccga ggcgaggatc ggcatggtcg tacaaatggg tgccatcgaa ctgggagaga
     9541 ccatgggcat cgtctggaaa atgtgcggga gcccaatcca tcaagacacc gattcctgct
     9601 tgatgcgccg catcgacgaa agccatgaaa tcttcaggag tgccgtagcg gctcgtcacc
     9661 gcaaaataac cggtcgcctg gtagccccag gaagcatcga acggatgttc ggtcaccggc
     9721 atgagctcca catgcgtgta tcccatatcc ttcacgtaag ggatgagctt tgcggccagc
     9781 tcccgatacg tcaaccagcg attgtgctct tcaggaatgc gcatccacga gccgagatga
     9841 acttcataaa ccgtgacagg tacctcgagc ggattttggt gacgccggtt ctccatccac
     9901 gcctgatcgt tccaccgata ggccgagatg tcccgtgcta ttgacgcggt tttgggtcgc
     9961 agctccgatg cgacggcata ggggtctgct ttcagcagaa aaccgtcacg gccacgaggg
    10021 cggatctcat acttgtagat tgtgccgtct tcgagcccgg ggatgaagag ttcccagagg
    10081 ccggttgctc cgcgattggt catgggatgc cgccggccat cccattgatt gaaatcgccc
    10141 accacactca ctcggccggc attgggggcc cagacaacga agtggatgcc tgggatgctg
    10201 tcaatcctgc gcacgtgcga cccgagcgtt tcgtaggctt tgaaaaaggt accttcagca
    10261 aaaagatgta aatcgaagtc cgagaggagc ggggcgaagg catacggatc gtagcgctcg
    10321 gagagccggc ccgcatgatc gaccacgcga agacgataat gcgacggcgc cggagcaggc
    10381 ggcaggaccg cttcgaagag gccggactca tggatacggg ccatggggaa tgccacctcg
    10441 tcggaggtct gggccaacac caccgcttca cgggcctcgg gtaaaaagct gcgaatgacc
    10501 aacgcaggga cgccattctg ctggctcata tggggcccaa gaatggccag cgggtcccag
    10561 tgtgaacccg tgactaattg ttcgatgtgc tgtcgtgaca atgcggacat gacacactcc
    10621 tcgtactaat ccagcgattg aacggtcgag cactattatg aaagaagaaa gggcagattg
    10681 ccacaagccg ctcgacgcac gtccattgca agagccgccc gtcctcttga taatgcgacg
    10741 atcctagacg cacaggagac agcgccatgc atccgtcgga actttccatc gcgctcttca
    10801 tctttggact ggcctatgcg gtcatcatca ccgagcggat tcataaaacg atcgtggcgg
    10861 tgtccggcgc ggctctgatg atcgcgtccg gcgtggtgac acaagacgaa gcgttttatt
    10921 cccatgaatt cggcgtggac tataacgtcg tctttctgct gatcgggatg atggtcattg
    10981 tcaatatcgt ccgtgaaacg ggactcttcg aggtgctcgc gatttggtcg gctcaacgtt
    11041 ccggagcgaa gccgttacgc ctgcttatga tgttggccat tgtcaccgct gccctgtccg
    11101 ccatgctgga taatgtgacg acagtgttgt tgatggcccc ggtcacgttg gcaatcacca
    11161 agcgtctgag cctgaatcca gtgccgtatt tgatcataga ggcggtcgcc tcgaatatcg
    11221 gcggaactgc gacgttggtg ggggatcctc caaacatcat gatcgccagc aaggcggatt
    11281 tgggctatgt ggattttctc gtcgtccttg gaccgattgt gacggttatc atggcggtgt
    11341 tcctcgggtg tctcaagctg ttcttcggca cgatgcaggt ggctcctcat caggccaacg
    11401 cggtgatgca attggatgcg agggatgccg tccaggatcc cgtgttgttg cgacggtgtg
    11461 tatggcttct tgcgctgatc aacgtcgcgt tttgcatcca tggtgcgctt cacctcgagc
    11521 cggcgacgat cgcgttggta ggggcgagtg cattcatgct gataggacat gccacgaaaa
    11581 agacggatgg cgatgagttg tcctatctca cggacgtgga atggaagact attttctttt
    11641 ttatcggtct ctttattctc gtcggcggtc ttgtgaaggt cggggtgata cggttccttg
    11701 ccggtcagct ggtatccttt acacagggcc atcccgcagc cacgactttt tcagtgctct
    11761 ggggctccgc cattctttct gcgattgtcg acaacattcc ttatgttgcc gcgatgaatc
    11821 cactgatcgt cgacctggca cgctcagccc atcctgatgt gaccgactac accacgctgg
    11881 tccaccaacc ggacatcctg ccgctctggt gggcgttggc cctcggagcg tgtctgggag
    11941 gcaatggaac aatcatcggc gccagcgcca atgtcgtgat cgtggatctg gccagaaaag
    12001 ccggctatcc gatctcttac tgggactttt tcaagcttgg atttccggtg atggtcgggt
    12061 cggtgctgct gagcacgctg tatgtctggc tggtcttttt aaggtgacac cagccccgca
    12121 agcgcgaggc tggtatctcc acctgcatgc tatgtgaagc acgaggggcc ggttcggctg
    12181 tagtggacga gccggtcata ggacacggtg cagtctgtac aatagaactc tgacaggaag
    12241 acgtcctgct cctgaatgaa gcagcgttgg acaaactccg ccatcgtgca ccaatcttga
    12301 cctgctgatc catcccaatg ctgtttcttg actcgctgac acgctggaca gcggtatgtc
    12361 aggggtagat cactcatgtt cgactcctgt ggcttgctgt tgctcgatgc ctcggaaact
    12421 gtgcgaacgt catgagcgtt gcgcagtggc gtgccgcaat gccatgaaaa gaagatgagg
    12481 gatcaggcag tgggaatggg aggacgcagc aagccgtgct cgctcactgg cctgagcacg
    12541 gtcgtcgaac tctcgccggt tgacgaagcc agaagcaaag ggacgagatc cgaaagagtg
    12601 gtaggcagtg aaaagccttc gaggagcgga ctgtctgcct gatcgagtgg aaactccatc
    12661 gcccagaggg tcatggtgct gccgagcatg tacatgacca ggcagagaca caccagcccg
    12721 ataccacacc acatgatcct gccgaaccgc gatcgtcgtt caagcagcat ggggtctgct
    12781 cccaagaggg cgacagtcta ggatcgcgcg gacaatatgt caatgccacg gcagggagat
    12841 caggcggccg gcagaatctt cacgccggcc tcggcaaact tctgctcgat gccgaagcgc
    12901 agctcactgg ccacttcgtt cggatcaacg tccaaccgaa tctcgagcca ataatttaac
    12961 gtgaagatgc ggccctgatt gccgaattca tcgagcagca cttgcggctt gggcgtcttc
    13021 aggacctggc ggtggctcgc cgcgagaccg gcgagaagat ccatcaccaa ttgagtggat
    13081 gagtcatagg gagcgccgac tcgaagggag aagcgtccga tgcggctcga gtatgtccag
    13141 ttggtcagat ggcgctcgag aaaactgctg ttcggaatca aggtgacgac gcctttcgca
    13201 tctcggacgg tggacgaccg cagaccaatg gtcgtcacgc gcccgcgcac attttccact
    13261 tcaatgaggt cgccgacacg caagggtcgt tcaatcagca ggaggatgcc gctgatgaca
    13321 tttttcaata agttttgagc gccgaatccc accccgatgg caaatgcgcc gcccaaaaag
    13381 gcgaaaatgg tcaagggaat cttgacccag acgaggctgg tcacgatcag gatggtgagg
    13441 agcagggcct ggctccactg gcgcaggaga ttcgccactt cgggggccat tgccaagcgt
    13501 ttgactgcga gccggccgat ctgcgcggcc aagtacaggc acagccagta tccgatcgtg
    13561 aggatgatca gggcggtgac gatcttgccg accgtgacgc tgcgatggcc ggtgattttc
    13621 ttgccgtcca cctcgatcgt gtcttctgcg ttgaatactt ccacgtgcca gatcgcgttg
    13681 gtgcggtccc aggcgtgctg ccaccactcc tccacacggg ccgtcatggg aagttctttt
    13741 tgctgtcctt taaatgccga tttccaacgc tcgagaaatc gagaagtctg gtcgagacgt
    13801 tggaggccat aattgtagat agtttcccgt tgccggtgtg cctccaggag tgcccgcacg
    13861 tggttgcggt cctggccggg tcggctgttt gtcacccgcg tttccaattc gccaatttgg
    13921 cccgagacga tactcagttg ctgccgcaca taatcccgtg acgcctgcaa attcgtgaag
    13981 agcggcgtaa agcgatcgta ggcttctcgg gcttttcccg gttcggggtt gtgcatgatg
    14041 gcgaagcggc tttcccacaa ctgtcgttcg ctgtctacca catcgagcag caactgccac
    14101 agatcgcttt tcagagcgag attgtcggcc cggatgcggc cgacttctgt gccggcttgg
    14161 atccggcgaa ggctctcaga cggttttttt ccggcttttc cctcaccctt tgccatccgg
    14221 gaagccagct gtcgctcgag gtcccgcaat gcctcgaact gcccgtgacg ggcggtggtc
    14281 gtttcttcga gttcgtgcgt caaagcttgc cgctcttgtg acaggcgcgt cctaattttc
    14341 tcgagatcgg gctctgtaaa ttcaaccagc tggtccgcgg ccgacaagcg actttccgcc
    14401 gcttgaaggc gtgcgcgtgc gtcggatagc tcgtctccaa tgcggatttt ggaaagctcc
    14461 gtcgcatgca gcaacgccgc tgcggcgcgt tgccgaagcg cttcaaggtc gcgtagccag
    14521 cgattccggt cggctcctga aggactggtc gtgctttcca aatgttcaga ggcttgccga
    14581 aggcgctcct cggcagcctg gagggcatct tttgcccggg tggagcgcag ttcgataaga
    14641 gccagttgcg actgaaggcc ttcgaccgcc aggcgcaggg caaaggcggt atcccacaat
    14701 cgatcggcat caaaaattgt agccggagta tcgccatgat ccttcgagtc gggcagagac
    14761 cgtccgacct catcccgatg ttggcgagcc tgttcgagac ggagggtagc gtcaatctgc
    14821 tcatcggcgc tgcgcacgat atggtgtagc agcgtccgcc gttctaacag ttccggttcg
    14881 ggcgtgcccg gaggagcgcc ggtcgacagc tggttcggcc ggctgaccgc atccagctct
    14941 ctttgagctt ggtctctctt ggcccgcagt tcgctcagcg acgaggcgtc gtcaggtggt
    15001 tgttttgagg cggcctcgga ggccttctgg ctcggttgat cgctcgtgtt gatctgtact
    15061 tcggcatgcg tgcgcacgcc gaagacggcg aacatgacga ccaccgcaag acacatgcga
    15121 atgttcatag aaagaaagaa atggggttgg atgtatggtt ggtatcacgg ctggcagggt
    15181 ggcaggactg gtgaaacccc ttggggtcgt ctccgcgggt cctcctctca cgtcgtcacc
    15241 agacgcgatc ttggaatcat tcttctgtgg attgtgtccg tcactccgct ctctcaaaca
    15301 atctttcctc gggcatttgc tagtcgatca gcgtctgagc ctccatcata gtgaatgaag
    15361 tggtgcgcgc gttgactgtt cggtcgcacg tcgcacgaca gcaaaactgg ctcatgactg
    15421 tccggctcaa gcccatcaaa gaacaagtga tcgtcatcac cggtgcctcg agcggcatcg
    15481 ggctcgtcac cgctcgcatg gcggcgaagc ggggggcccg cgtcatgctt acggcccgaa
    15541 accacgatgc ccttaaacag ctggcggatg aaatcgtggc gaatgaatcg aacggaggca
    15601 tggcggcttt tctcgctgcc gatgtggcgg atgaagcggc gttgcgccgc gtcgctgacg
    15661 aagccgtatc ccggttcggc cgaatcgaca cctgggtgaa cggcgccggt gtctcggtct
    15721 atggacgcct caccgacgtc tcattggaag accaacgacg tgtgtttgag acgaattttt
    15781 ggggcgtggt catcgggtcc cgcatcgcga tcccccattt gcgaactgga ggggccctca
    15841 tcaatatcgg gagtgtcctg tccgatcgag ccgtgcctct gcagggcgtg tattgcgccg
    15901 ccaaacatgc ggtcaaaggc tttaccgact cgttacgaat ggagttggag catgacgacc
    15961 tgccgattgc cgtcaccttg attaagccgt attccatcga taccccttat cctgaacatg
    16021 ccaagaatta tctaccgacc gagccgacgc tgccttttcc cgtgtacgcg cccgaagtcg
    16081 tggccgagac gatcctccat tgtgccgagc acccaacgcg cgatgtgtat gccggaggcg
    16141 gggcgaaaat catggctgcc atgggcggcc tggcacctcg cctgactgac aggctgatgg
    16201 aaaatttgat cgatatgcaa ctcaccgatc gtcctgaaga cgaccggacc aataacagcc
    16261 tctatggtcc cacaacaggc ctcaaggagc gagggggccg ggcggcgtac gtggctgagt
    16321 ccagcttgta tacacaagtc tctctccatc cgctgctcac gggggccgcc ttagcagcca
    16381 ctgggctgac attggcctcg tggttgttcc gacggacgtc ggcggcgaag tacgagccga
    16441 ccggtcacca ttggtatgag gctgacagag tgaaacacta gcctgaggga gtgcggccgc
    16501 cgtgatgcag acaataactg ggtggctcct gttagcaacc gcagccgcgt ttctcccagc
    16561 cacaggggga caggaggcaa agcgggacac cctctctacc gctcaatcgt ttctcatgaa
    16621 agcggcggaa ggtcagcaag ccgagatcga gatggggcgg ctcgcctcgc agcgtggagg
    16681 agctgagcag gtgaaacagt ttggagcaca gatgatggaa gatcatcaaa aagccagcaa
    16741 ggagattcaa ctcctcgcca gcaaggaagg cgtcgtgctt ccgaccgagc tcacgggcaa
    16801 gcacaaagac aagcgagagg agtttgggca attgtccggg gacgcgttcg atcgggcgta
    16861 catccgctac atgcttcgtg atcatcagaa ggacgtcaag gagtttgagc ggcatgccaa
    16921 agcgatcaag caccccccgg tccaacgatg ggcggaaggg actctgccgc tcctcaaaca
    16981 gcaccttcag accgcgcaaa ggatcgcctc ctccatcggc gtagagggtg tcagataaca
    17041 ggcgcgagaa gaaacgttcc aagcgaggga aacgatgaag atcaaacagg tcgggctgaa
    17101 tagagtgctg atagtggcgg gagcaattgt cgctgtctcg atgattgtca gtcaggctga
    17161 gaggaccggc ctggcacagc ccactcccga agggaagaag cagcaagcgc aaatgggcac
    17221 catcgaggag acgaagcaaa aaggctttga aggggtggat ttttcctatg acgtgttcgg
    17281 ggcgccgccc ggtcaggatc cccaaaaaat tgccgacgag gtgatgaaga aggacatcgc
    17341 gcaaaagccc gacgtcatga agaaacaaca caagttgctg agcgaacgct acgacttgaa
    17401 ttgcaggacc gagaagggca ttgccatgtc acgcggaaag ccgcagccca tcgggccggc
    17461 cgtcaagctg gcgtcaggaa tgacatggga ccaactcgcc caactgtcgc cggaagacat
    17521 caagaagaag ggagtgtttc cacagggatt cgatcggctg ccgcatgtca agcacgcggt
    17581 cggcggtcag gtctttcccc aggtgcagat caaggaattt ccgagactcg aacgatttga
    17641 cgtcgatttt gacctgccgg aatgcctgct ccccgaattt cctccgccga ttttcttgac
    17701 gacccatccg gagctggggg atgtctctca gggggaggtg gtccatgcgg aaaattttga
    17761 ccggcttttt cgaggtcttg tgactcccgc gcagctggac gggctgcgca tgctggtcac
    17821 gcagtttgca caggaagagt tcaatgccac ccacgaccgc aaaaccgcca agccgagttt
    17881 gggcgtgagc tgtctcgatt gccacgtcaa tttccatacg accgggcaat ttcacttgaa
    17941 cccggatacg cgaccgcaaa tggagcgcct gcggttggac acggtcagcc ttcggggcat
    18001 gttcaaccag caaattcacg gctcgaagcg ttcaattcgg tctgtggagg acttcacaga
    18061 atttgagcaa cgcacggcct acttcaacgg ggacccgatc cgtgccatga aaaaagggat
    18121 gaatatcatc gaccgcgtcc cggtcgcgca tatggcgcaa atgcaaaaca tgattgattt
    18181 cccccctgcg cccaaactcg atccgctgac cggccgcctc gaccgcacga aagcgacgcc
    18241 gctcgaaatc aaaggagaag atctgttttt cgggaaggcc aagtgtgcgg cctgtcatcg
    18301 tccgcctgcg ttcaccgatc atcacatgca cgatctccat ctggaacggt ttggtgcagc
    18361 agcggacggg ccgatcaaaa catttgtgct gcggggcatc aaggattcac cgccgtattt
    18421 tcacgacggc cgtcttccca ctctcgaaga cgtggtggag tttttcaatg tgatcggagg
    18481 gctgaaactt gaccaggacg agaaaaaggc cctcgtcgcg tttatgcgcg cgttatgacc
    18541 aatgccacag taagggagag gacacatgga ggaccaaatg gatgggacag tccttcgtcg
    18601 gatcggagaa ctcgtctcac tggaacactc gtttcaggaa caggagttgc tgtccgatga
    18661 cgatcgggca agactgcgcg gcgtccaagt agaactcgat cagagctggg atctcttgcg
    18721 gcagcggagg gccttgcgtg ccgcgggaca agaccccaat aaggcccaag tcagaccggc
    18781 agggatcgtt caaaagtacg tgcaatgatg ttgatgagct taactcacga gaaaggagga
    18841 cgacatgtcc gtcattgaaa agtcgatcga aatcaatgtc cccgtgcggg ccgcttataa
    18901 ccagtggacg caatttgaag agtttccgaa attcatggaa ggcgtgaagc aggtcaagca
    18961 actcgacgac aagcatttgc attggaaagc cgatattgga ggaaaagaaa aggagtggaa
    19021 ggcagagatc accgaacaga ttcccgaaga gcgcatcgcc tggacgagca ggggaggcgc
    19081 catcaatgcc ggtgtggtga cgtttcatcg tcggtcggac tcaaaatcga aagtcatgct
    19141 ccaaatggaa tatgacccgc agggttttgt cgaaaacgtc ggggatgccg taggggtcgt
    19201 cacgcaacgg gtacaaggtg accttgagcg gttcaagcaa tacattgaaa atcgggggca
    19261 ggagaccggc gcctggagag ggacggtgaa gtagccgccg tggctcgtat tgtctttcga
    19321 cagcttcaaa ggtcagccgt gccggactcc aacggccggt tctggcccag gacgatcgcc
    19381 gccggctgcc tgtccggcat gacagtggcc ggagcggcgg cctggcgtgc gcaaatggaa
    19441 gggcaatccg gctggggacc gatcaacgcc atcagtcaca tcgtctgggg acctcaggcc
    19501 gccgcgcaac agcggttcac cgccaagtac acgggctcag ggcttttgtt gaacgctctg
    19561 gcctgcggat tttgggggtg gctgtatggc gtgatgggac gatacaggcc tgcaccgcat
    19621 tcgtattcaa ggtcagccgg ccgagggatt gtcgtcgcag ccttggccta tatcaccgat
    19681 tactatctgg ttcctcgtcg tttcacgccc ggctttgagt tgagcctgac acgccggagc
    19741 ttcccctggc tgtatggagc actcgctgtc ggtttgatct tgcccgattg ccttgtaccc
    19801 ccgaaatcaa ggagtcgatc atgaacggac aacggctcgt gacttatatg tgcgcatgca
    19861 tgctgcttca ggctgttcct gtgttctgcc aggatgccat cttgcctcag ggcgatcctc
    19921 gtctgcgaga cgaccctcaa gaagcgaagc agcgtgagga gattcgcgac cgccggatgg
    19981 gacagcaggg acaggggccg gactatcgtg tggagggcaa gccgcaaggg ggcaccagcg
    20041 gcgccaccgc cggcgaggcg aacggcagga ctcggcagga caccggtctg tcggatccaa
    20101 gtgtgaatcc cggtcaggct tccggtatgc gtgtattgcg tggcgaagtg aagcaggcga
    20161 atccaaaatc cattgtcatc gaggacagac acgggaaaga gacgacaatg tcggtggatg
    20221 ccgcgacggc cggagaccgc gatattcgtc ccggcgactt catcaccgga accgtgacga
    20281 cccaggggcg cgccgttgcg attcataagg agagcgcacc cggccggtag cagatccagc
    20341 aagcacttcc gctccaacca cctctggcta ttgagccgtc gatgtcgacg cttaagattg
    20401 aagacaagga gacgcgatga ccacaagatc tctccaagga acatccccga aaacgacacg
    20461 gccgggcaag agcagctcga agacacagga aggcagtcct gcaaccggcc aacagctaag
    20521 taccgtcagt gaccgcctga gggaaaacgg taacggtaac ggatccctga cccaggaaga
    20581 gcagttggcc tatggcctcg gatggtttgg aatcgggctg ggcctcacgg aactcatgat
    20641 gccacggcga ctggcgcacg cgaccgggct acccgttcga caccgcggtt tgatccggat
    20701 aatggggttg cgcgagatcg cgagcggtct gggaattatc acccaacgca gacctgccgg
    20761 tgccgtctgg tcccgagtgg ccggcgacgt catcgatctg gcttgcctcg gcgtcgcatt
    20821 cacttcggcg ggcgccgatc gcaaaaaact cgcggctgcc gtcactgcgg ttgccggagc
    20881 gacggcactc gatctgctct gcgcgcagca actcacccga gacgtggtga cccgcaacgg
    20941 gacgatagca gtcaccgtca gtctcatcat cgatcgctcg ccgggggatc tgtacagcta
    21001 ttggcacgac ttcaccaatt tcccctcgtt catgaagcat gtcatctcgg tccaagtcac
    21061 tggcgagcgt cgctcacact gggtggtgac tggtcctgcc ggctccacgg tcgaatggga
    21121 tgccgaagtc actgaggatt ctgccgacac gctcattgcg tggcggaccc tcgaggggtc
    21181 ggatgtagac catcgtggtc aggttcgttt tgagccggca ccgggaaaac gcggcacgtt
    21241 ggtcaccgtt gatttgcagt accgtccacc ggcaggcacg cttggctccg ccatcgctac
    21301 ctggtttggt cagaaccccg agcactccat gaagatggat ctgcggcgat tcaagcaggt
    21361 gatggagacc ggcgaggtta ttacgacaga ggggcaaccg gcagggagac aggggagcac
    21421 ctcgtggaag tatgaccgcg cggtgcgcgg atcaggaaca cctcgaacgc gtggacgaac
    21481 atgaggccca attcgcgcgt cttgccctcc aagacgagga attgcaggaa ggagaacgat
    21541 gaaagctaat tgttggtatg gaaaacgaca tgtgcaggtg gttgatgtgc cggatcccat
    21601 aattctcaat cagcgcgatg ccatcgtcaa aattacgtcg acggcgattt gcgggtccga
    21661 tttgcacctc tataacggct tcgtgcccac gatggaacaa ggcgacatca tggggcatga
    21721 attcatgggt gaagtggtgg aagtggggcg ggaagtgaaa aagctccgcg tgggcgatcg
    21781 agtggtggtc gcgtttccca tttcgtgcgg caattgcttc ttttgcaagg gtgaaatgta
    21841 ttcgctatgc gagaattcca atccgaatgc ctggatggcg gagcaatttt ttggccattc
    21901 gccagccggt atcttcggct attcccatct caccgggggg ttcgccggtg gacaggcgga
    21961 atacgtccgc gtccctttcg ctgatgtcgg caccgtcaaa attgaaaatg gcttgtccga
    22021 cgaacaagtc ttgttccttt ccgacatttt cccgacgggg tacatggccg cagaaaactg
    22081 caatatctcc ccgggcgaca cggtggccat ttgggggtgc ggtcccgtgg gccaatttgc
    22141 gatccggagc gcgttgatgc tcggcgctga gcgtgtcgtt gcgatcgacc gcgtgccgga
    22201 acggctcaag atggcccgtg acggaggcgc ccacacgatc gactatgagg agaccgacgt
    22261 ctacgacatg ctgatgcagt tcacaggggg acgagggcct gattcatgta tcgatgcggt
    22321 tggacttgaa gcccatgcac ctaccccgtt gtattactac gatcgtctca aacaagccat
    22381 gatgctggag tccgaccggc cgattgcgct gcgtgaggcc atcatggcct gccgtaacgg
    22441 gggaacggta tcgattccgg gcgtgtatgg aggcctgatc gacaaaatgc cgatgggggc
    22501 ggtgatgaac aaggccttga ccctcaaaac gggacaaacg catacacaac gctatctcaa
    22561 accgctgctc aaacggatta aggacgggga catcgatccg agttttgtcg tgacgcaccg
    22621 gttgaagctg gctgacgctc ctcagggctt cgcgacgttc aatgacaagc aggacggttg
    22681 tatcaagatc gtgatgactc cctgacgcat gccaccgaag gagtacagat gaaacccgta
    22741 tggtccacgt ctctgttggc ggggatgatc ttgtggtgtg caggagacgt gttggccctc
    22801 gcagaagtga agtctgacgc ctccatggaa gccttgcggt gggccaaaca ggcggaagcc
    22861 tcaggcgtgg cacgcgatca ggcacgcttt gtcgaggaaa tcgacagagc gttgcaggtg
    22921 gccctggatg ccggggccaa gacgccgaat caccgcttgg ccgaagcggt gcatgcgctg
    22981 atggaggccc gggcacttgg tcagcaaggc aacctggcgg aggggcgtga ggatttgcaa
    23041 tcggccatcg tctggctcac ccgcgcagcc gggcttccgg cagccaaccc gtcagccgac
    23101 tggtcgatga accaacgaga ctcaacatta ttgaagggcc aatcggcccg accaggaaag
    23161 gaacacccga tggatcattt cttaattgac atcgaagagg cgacgattcg caacaacaac
    23221 tttcgtcagg tgttgttcac cgcctcccac agtcagctgg tcttgatgag tttgaagccg
    23281 ggagaagaga tcggcaccga gacgcaccat ctcgatcagt tcatccgtgt cgaggcaggc
    23341 gaaggcagtg cccgcctcaa cggcaaggat tacgcgctga aagacgggag cgcacttgtc
    23401 atcccggcgg ggactgaaca caacgtcatc aacgaccgca aagatcagcc gttgaagctg
    23461 tataccattt acagtccgcc agaacacaag gacggggtcg ttcaccggac gaaagaagag
    23521 gcggaagcgg accgcgatga ccacttcgat ggaaagacca cggcatcatt gcacgaatcg
    23581 ccggcgtctc ggtaagcggg ctatcatgcg gcgtcgaccc caaccgagtg gcgtcagaaa
    23641 ttacgttgag cagggagtga agggattcgc agcttcgtct caatttctcg ggtacatcct
    23701 gtccagttct tagacagcct gtctgaacga ctcgggtgct gcctagtcgc tcgactattc
    23761 gaaatcccga gaccggccgt tttccggtcc gcaggaatcc gccga
//
LOCUS       DASSCQ010000021         8278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_383327, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000021 DASSCQ010000000
VERSION     DASSCQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383327"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VFH05_01485"
     CDS             <1..1205
                     /locus_tag="VFH05_01485"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET6676917.1"
                     /translation="VSLAPALQQDDLSKRLQLATDQFIVKPGARPEEQAIAQASGDDA
                     RTVIAGYHWFGDWGRDTMISLEGLTLSTGRQQETRAILRTFARYIQDGLIPNLFPEGS
                     RQALYHTADATLWYFHALDRYLSATDDRETLALLYPRLEEVVHHHRKGTRFNIGVDKR
                     DGLLAAGAPGYQLTWMDAKVDDWVVTPRRGKPVEIQALWYNALRLMIQWGSQLGAKTD
                     LWLQMAEQAEASFHQRYWIESGGYLFDVVDGEKGDDRSLRPNQIFSLSLSHPILRPER
                     WRSVVDIVSERLLTPFGLRTLASDHPDYKPTYSGDLRARDAAYHQGTVWPWLIGHYID
                     ARLRVYHDKAESRQLLVKFNDHLHDAGIGTISEIFDAEPPFRPRGCIAQAWSVAEVLR
                     AYLKTKEA"
     gene            1206..1967
                     /locus_tag="VFH05_01490"
     CDS             1206..1967
                     /locus_tag="VFH05_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6676918.1"
                     /translation="MTQGTPARHQSQCSVHIGTSGWHYKHWVGPFYPTDLPPKAMLSW
                     YVRQFDTVELNNSFYHLPKPETFEAWKRATPPSFCFAVKGSRYITHRKKLNDPETAVA
                     RFLEPVQVLGDKLGPILFQLPPRWSCNLPRLSAFLESLPRTHRYTFEFRDPSWHDAAV
                     YKTLGRFNAAFCLYELDGFEAPYQLTADFVYVRLHGPGRKYQGDYSTKQLQDWAERIT
                     RWKRELVAVYVYFDNDQAGYAAKNAADLKRMVAGS"
     gene            2039..2506
                     /locus_tag="VFH05_01495"
     CDS             2039..2506
                     /locus_tag="VFH05_01495"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET6676919.1"
                     /translation="METRASVADHPIHPMLIPFPIALWIFSLASDCIYLFDFGGPVWK
                     DVALYTMIGGIIGALAAAIPGYIDYRSLTDPVTTRVAQMHMFINVGLVLLYTINAGLR
                     VLNGPEAVLPVILSFVGVAALGVSGWLGGELVYVQGVAVQPERESSGPRRRAA"
     gene            2613..4364
                     /locus_tag="VFH05_01500"
     CDS             2613..4364
                     /locus_tag="VFH05_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6676920.1"
                     /translation="MADTVADLLVDRLIDWEVDTVFSLPGDGINGIFESLRRRQGRIK
                     VIQVRHEESAAFAACGYAKFSRRLGVCLATSGPGGLHLLNGLYDAKCDGQPVLAITGH
                     TFHDLIGTHYQQDVDLVKVFADVAAYNERVMGPAHVCNVVDEAIKTAIARRTVAHITI
                     PKDIQDWSADERRSKANVPMHSSDLYSEALPLPPHATLQQAADILNGGSRVAILAGRG
                     CLTARKEIVAVAETLAAPIVKPLLGKAVVPDDHPYTTGGIGLLGTAPSQEVLEECDTL
                     LIAGSSFPYLEFYPKPGQAKAVQIDLDPSRIGLRCPVDVGLVGHCWDVLRALLPLLTR
                     RSDRTFLSKAQDRMRQWNDLMEMRGTRNDVPLKPQVVARALNEFLDRDAIICSDTGTV
                     TTWAARHIMIRGDMEFSVSGTLATMANALPYSIGAAVAFPGRQVVCFTGDGGMTMLMG
                     ELATIAKYRLPVKVIVIKNNVLGMIKWEQLAFEGNPQYGVQLHPIDFVSVARACGVGG
                     YNVDDPALVRAVIGEAFHHPGPALVEASVDPNEPPLPGKITTDQAWQFAKALAKGQKD
                     RWDIIKTIVENKIREVV"
     gene            4481..6628
                     /locus_tag="VFH05_01505"
     CDS             4481..6628
                     /locus_tag="VFH05_01505"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HET6676921.1"
                     /translation="MAHGISKAIVIKNGNLFFLAPPEGEVPMTRDHGFGLYYHDCRYL
                     NGYELLVGGEHPEPLAARSWEGGRAVFQLTTPDFRAPDGRMIDRETVGLKWERVIDAE
                     QCVLHEQLDIRYFGHEAVDFPITMRFRAGFEDVFEIRGLIRKVKGKLFKPYWKDKCLY
                     FSYEGGDRIRRSLAVHFYPLPTQVKGNSVQFDIALKSNETKQIRISLALAESEEQPNR
                     LFAQTSVYRDLHAITQGIKASCEQWMRRETEFHSDSLLLNRVMRRSLMDLHMLKTSIG
                     GDEFFAAGVPWYVTLFGRDCLVTSLQTLAFWSDTAAATLRLLARYQGAEVNDWRDEEP
                     GKILHELRVGELARMNKIPHTPYYGTIDATPLFLILMSRYAHWTGDLTLFEELRTPIE
                     RALHWIDKYGDIAGDGYVSYVGNTNKGLLNQGWKDSGDAIVTEDGRLAEPPIALVEVQ
                     SYVYMAKMEIADLYERVGEREHAVRLRAEAGALAARFNHDFWVEEEGCFALALEAEHR
                     PCRVMSSNAGHALWTGIVDKQKAERVVHRLMRPDMFNGWGIRTLSYKERRYNPMGYHL
                     GTVWPHDNSLIAAGFRRYGYQEEASRIFVGLLEAAMEFEDYRLPELFTGFGREEYGLP
                     VRYPVACHPQAWAAGAIPFIVETFLGLIPDAFNTRLKVVKPFLPEFINQVELRHLRIG
                     KASVDLRFERKPDGSLHAQVGQVTGDLRVEVED"
     gene            6719..8029
                     /locus_tag="VFH05_01510"
     CDS             6719..8029
                     /locus_tag="VFH05_01510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET6676922.1"
                     /translation="MTESPTIRSAMVFFEELLRGCETPNIAVRFWDGSVWRLREDERI
                     EATLVLRHPDSLSRMLRWPVQLALGEAYIYDDIDVLGSLDALLPLADHLMKRRWSLMD
                     WLRCGVATWRHRNLDRLPEETRNLALHGRRHEKPRDKAAMRFHYDVPVEFYRLWLDSR
                     LLYSCGYYQHEEDDLEQAQFQKLEYLCRKLRLNPGDRILDIGCGWGGFALYAAQTHGA
                     LVHGITLSRRQAEVANHRITAAGMSRRCRIEVRDFRDVHGDQLYDKIISIGMVEHIGT
                     DRLAPYYERALGLLSPGGVFLNQGIGTRDGEPALGAFAQRYVFPDAELVPIQDIVRTA
                     ESCGFEVCDVESLRQHYALTLDAWGQRLERHHQEALRAVGEVTYRVWRAYMAMAAYLF
                     RQGRLSLYHTMCVKAVDGHTDLPLTRKDWYESRISEARVRQEAA"
BASE COUNT         1707 a         2346 c         2508 g         1717 t
ORIGIN      
        1 tggtgtccct tgccccggcg ctccagcagg acgacttgag taagcggctc cagttggcca
       61 ccgatcagtt catcgtcaag ccgggagcgc gaccggaaga gcaagccatc gcccaagcct
      121 ccggtgatga tgcgcggacc gtgatcgccg gctatcattg gttcggcgac tggggacgcg
      181 acacgatgat cagcctggag gggctgacgc tgtctaccgg caggcaacag gaaacacgag
      241 ccatcctacg aaccttcgct cgctacatcc aggacggact cattcccaac ctgtttcccg
      301 agggatcacg ccaggcgctc taccacacag ccgacgccac actctggtat ttccatgccc
      361 tcgatcgata tctttcggcg accgatgatc gagaaaccct tgccttgctg tatccccggt
      421 tggaagaagt cgtgcatcac catcgcaagg gcacccggtt caacatcggt gtagataagc
      481 gtgacgggtt gttggctgcc ggagcgccgg gataccagct cacctggatg gacgcgaagg
      541 tcgacgattg ggtggtcacg cctcgccgag gaaagccggt cgaaattcaa gcgctctggt
      601 ataacgcgtt gcgattgatg atccagtggg ggagtcagct tggagcaaag accgatctgt
      661 ggcttcagat ggccgaacag gcggaggcct catttcatca acgctactgg atcgaatcgg
      721 gcgggtacct ctttgacgtg gtggacggtg aaaagggcga tgaccggagc cttcgcccga
      781 atcagatttt ctccctttcg ctgagccacc ccattctccg tcccgagcga tggcggtcgg
      841 tggtcgacat tgtcagtgaa cgcctgttga cgcccttcgg gttgcgcacc ctggcctcgg
      901 atcatccgga ctacaaaccg acctattccg gagatcttcg cgcccgcgac gccgcgtacc
      961 accaggggac cgtatggccg tggctcattg gtcattatat tgatgcccgg ttgcgggtgt
     1021 accacgacaa ggcggaatcc cggcaactgt tggtcaagtt caacgatcat ctgcacgacg
     1081 ccggcatcgg cacgatcagc gaaatatttg atgctgagcc gccttttcgg ccacggggct
     1141 gcatcgccca ggcctggagc gtcgcggagg tactcagagc gtatttaaaa accaaagagg
     1201 catgagtgac acagggaaca ccggccaggc accagtccca atgttccgtg cacatcggga
     1261 cgtccggatg gcactacaag cactgggtag gaccgtttta tccaaccgac ctgcccccca
     1321 aggctatgtt gtcctggtat gtccgacagt tcgacaccgt ggagctgaac aacagcttct
     1381 accatttgcc gaagccagag acgtttgaag cctggaagcg ggcgactcct ccgtcattct
     1441 gttttgcagt caaaggaagc cgctacatta cgcatcgcaa aaaattgaac gatcctgaga
     1501 ccgccgttgc ccgctttctg gagccggtgc aggtactcgg tgacaagctc gggccgattc
     1561 tttttcaatt gccgccacgg tggtcgtgca acctcccgcg tctcagcgcc tttctcgaat
     1621 cgctgccgcg gacccaccgc tataccttcg aattccgcga tcccagctgg catgatgccg
     1681 ccgtgtacaa aacgctgggc cggttcaatg ccgctttctg cttgtacgaa ctcgacggct
     1741 tcgaggcgcc gtaccaactg acggcggact tcgtctatgt gcggcttcat ggtccgggac
     1801 gtaaatatca aggggattac tcgaccaagc aattacagga ttgggcagag cggattacgc
     1861 ggtggaaaag ggagctggta gccgtgtacg tgtactttga caacgaccag gctggatacg
     1921 cggccaaaaa tgccgccgat ctcaaacgga tggtggccgg ctcatgacgg gtatctccgg
     1981 caggcccgtc tgccggctca gacttcacat gctcgagccg attccgagga ggtgacccat
     2041 ggaaacacgt gccagcgttg ccgatcatcc tatccatccc atgctgattc cctttccgat
     2101 tgccctgtgg atcttttcgt tggcgagcga ctgtatttat ctgtttgact tcggcgggcc
     2161 ggtgtggaag gacgtggccc tgtacacgat gatcggcggc atcatcggcg cactcgcggc
     2221 tgcgatacct ggctatatcg actaccggtc actgacagat ccggtcacga cgcgcgtcgc
     2281 gcagatgcac atgttcatca acgtcgggct ggttttgctg tacaccatca atgcgggatt
     2341 gcgtgtcttg aacggcccgg aggctgtgtt gccggtgatt ctctcgttcg taggggttgc
     2401 cgcgctcggc gtgtcggggt ggctgggggg cgagttggtc tatgtgcagg gagtcgccgt
     2461 ccagccggaa cgggaatcct ccggtccaag gcgacgagcg gcataaccaa caacgaactc
     2521 agcctgtcgg atcatccgca tattcgatga gatagatgcc caagccggca ggcagatagc
     2581 ggcagtcacc gctgtttgga atggagattg atatggcaga caccgttgca gatctgttgg
     2641 tggatcgcct catcgactgg gaagtggaca cggtcttcag tcttccaggc gacggcatca
     2701 acggcatttt tgaatccctc aggcgtcggc agggccgcat taaagtcatt caagtgcgcc
     2761 acgaggaatc cgccgccttt gcggcatgcg gctatgcgaa attctcccgg cggctcggcg
     2821 tgtgcctggc gacatccggc cctggtggac tgcacttgct gaacggattg tatgatgcca
     2881 aatgcgacgg ccaacccgta ctcgcgatta cggggcatac gtttcatgat ctgatcggca
     2941 cgcattacca acaggatgtc gatttggtca aagtctttgc tgatgtcgca gcctacaacg
     3001 aacgggtcat ggggcccgcc catgtctgca atgtagtgga tgaggccatc aagaccgcca
     3061 tcgcgagacg gaccgtggct catatcacga ttcccaaaga catccaggat tggtctgctg
     3121 acgagcgtcg atcaaaggcg aacgtcccca tgcatagtag cgatttgtac agcgaggcgc
     3181 tgccgctacc gccgcatgcc acgttgcagc aagccgccga tatcctgaac gggggatcgc
     3241 gtgtagccat tctcgcggga cgcggctgtc tgacggcgcg aaaggaaatc gtggccgttg
     3301 ccgagacgct ggctgcgccg atcgtcaaac cgctgctcgg caaagccgtg gtgccggacg
     3361 atcatcccta cacgacaggg ggaatcggct tgctcgggac cgcgccgtcg caagaagtct
     3421 tggaggaatg tgacacgctg ttgatcgccg gcagcagctt cccctacctc gagttctatc
     3481 ccaagcccgg ccaggcgaag gccgtgcaaa ttgatctcga tccttccagg atcgggctgc
     3541 ggtgtccggt cgatgtcgga ttggtcgggc attgttggga cgtgctcagg gccttgttgc
     3601 cgctcttgac ccgacgatcg gaccgcacat ttctttcgaa ggcgcaggat cgcatgcggc
     3661 agtggaatga cctgatggaa atgcgcggca ccaggaatga tgtcccgctc aagccccagg
     3721 tcgtcgcgcg tgcgctgaat gaatttctgg atcgtgatgc gatcatctgc agcgacacgg
     3781 gtacggtgac gacctgggcg gcgcgtcaca tcatgatccg tggagacatg gaattttcag
     3841 tatcgggcac actggcaacc atggccaatg ccctgccgta cagcatcggg gccgcggttg
     3901 cgtttccggg acggcaggtg gtctgtttta ccggagacgg cggtatgacc atgttgatgg
     3961 gagaattggc gaccatcgcc aaataccgtc tgccggtcaa ggtgatcgtg atcaaaaaca
     4021 atgtcctcgg catgatcaaa tgggaacagc tggcgtttga gggcaatccc caatatggtg
     4081 tccagctaca tccgatcgac tttgtctccg tcgcccgagc ctgtggggtg ggcggctaca
     4141 acgtcgacga cccggcactg gttcgggcgg tgatcgggga agcgtttcat caccccgggc
     4201 ccgcgctggt cgaagccagc gtggatccca acgaaccgcc gctccctgga aaaatcacaa
     4261 ccgatcaggc ctggcaattt gccaaagccc tggccaaggg acagaaggac cgttgggaca
     4321 tcatcaagac gatcgtggag aacaaaatca gggaggtcgt gtgatgcgcc gatgcggctc
     4381 gggcacaaca tggttgagac gcatcattgg cgtttttccg aaagagagtc gaaagagaat
     4441 caaaggcgga tacaaccggt tctgacgagg agcgtcttct gtggcacacg gcatttcaaa
     4501 agcaatcgtc atcaagaatg gaaacctgtt ttttcttgca ccccctgagg gcgaagtgcc
     4561 gatgactcgc gaccatgggt tcggtctgta ttatcacgat tgccgatatc tcaacggcta
     4621 tgagttgctg gtcggcggcg aacatcctga acctcttgcg gcacgctcat gggaaggagg
     4681 gcgcgccgtg tttcaattga ccactccaga ctttcgagcg cccgacggca gaatgatcga
     4741 cagggaaacg gtcggcttga agtgggagcg cgtcattgac gcggaacagt gcgtgctgca
     4801 cgagcagctc gacatccgct acttcggaca tgaagcggtg gattttccca tcaccatgcg
     4861 atttcgcgcc ggatttgaag acgtgttcga aatacgcggg ctcatccgca aggttaaagg
     4921 caaattgttc aagccctact ggaaggacaa gtgtctctac ttttcatatg aaggaggcga
     4981 tcgcatccgc aggagtctcg ccgtgcactt ttatcccctt cccacccagg tcaaaggcaa
     5041 ctccgttcag tttgatatcg cgctcaagtc gaatgagacc aagcagatcc gtatctccct
     5101 tgccctcgca gaatcggagg aacagccaaa ccggctgttc gcacagacca gcgtgtatcg
     5161 agatttgcac gcgatcactc agggcatcaa agcgtcctgc gagcaatgga tgaggcgtga
     5221 aacggaattt cacagcgaca gtctgttgct caaccgcgtc atgcgccgat cgctgatgga
     5281 tctgcacatg ctgaaaacat caattggcgg cgacgaattt tttgccgccg gcgtgccgtg
     5341 gtacgtcacc ttgttcggac gggactgcct cgtcacgtcg ctgcagacac tggccttctg
     5401 gtctgacacg gccgcggcca cactccgctt gctcgcgcgc tatcagggag cagaggtcaa
     5461 tgactggcgg gatgaggaac ccggaaagat cctccacgag ctgcgcgtcg gcgagttggc
     5521 gcggatgaat aagatcccgc acacgccgta ctacggcacc atcgatgcga ctccactctt
     5581 cctgatcctc atgagccgct acgcacattg gaccggtgac ctgaccctgt tcgaagagtt
     5641 gcgcactccc atagaacgcg cgctgcattg gatcgacaag tatggcgaca ttgccggcga
     5701 cgggtatgtc agctatgtgg gcaacacgaa caagggcctg ctcaatcaag ggtggaagga
     5761 ctcaggggat gccatcgtga ccgaggatgg tcgattggcc gagccgccca tcgccttggt
     5821 cgaagtgcaa agctatgtct acatggccaa gatggaaatt gccgatctct atgaacgggt
     5881 cggtgagcgc gaacatgccg tgcggttgcg ggcggaagct ggtgccttgg ctgcccgctt
     5941 caatcatgac ttttgggtgg aggaggaagg ttgttttgcc ttggcgttgg aagcggagca
     6001 ccgtccttgc cgagtgatgt cttccaatgc cggacatgcg ctgtggaccg gcattgtgga
     6061 caagcagaag gccgagcggg tcgtccaccg cctcatgagg cccgatatgt tcaacgggtg
     6121 ggggatccgt acgttgtcct acaaagagcg ccgctataac ccgatggggt atcacctggg
     6181 caccgtctgg ccgcacgata attcgctcat cgcagccggg ttccggcgat atggatatca
     6241 ggaggaggcc agccgtatct tcgtcggact tctcgaagcc gccatggagt tcgaagatta
     6301 tcgtcttccg gagttgttca cggggtttgg gcgcgaggaa tacggcctac ctgttcgata
     6361 ccccgtcgca tgccatccac aggcatgggc ggccggggcg attccgttta tcgtcgagac
     6421 atttttaggc cttatccctg atgcgttcaa tacgcgattg aaggtggtga agcctttctt
     6481 gcctgagttc atcaaccagg tggagctccg ccatttacgg ataggcaagg cctccgtgga
     6541 tctacggttt gaacgcaagc cggatggctc tctccacgcg caggtcggac aagtgacggg
     6601 cgatttacgc gtggaggtcg aggactgagg acaggcgcct cgcaccgccg gcctggaggt
     6661 ggtcactatg ctatctcata taaaggattc tcaaccggat gaggatgagg ctccggacat
     6721 gaccgaatcg cccaccatcc ggtcagcgat ggtgttcttc gaggaactgc ttcgcggttg
     6781 tgagacgccg aacattgccg tccgtttctg ggatgggtct gtctggcggc tgcgggaaga
     6841 cgaacggatc gaggccacgc tggtcttgcg gcatccggat tccctgagcc gcatgctccg
     6901 ctggcccgtg caactggcac tgggcgaagc gtacatctat gacgacatcg acgtgctcgg
     6961 ctcgcttgat gcactcttgc ccctggcaga ccatctcatg aagcgccgct ggtcgctgat
     7021 ggactggcta cgatgcgggg tggcgacctg gcgtcacagg aacctcgatc gcctgcccga
     7081 agaaacgcga aacctagccc tgcatggccg ccgccatgaa aagcctagag acaaggcggc
     7141 catgcggttt cactacgacg tgccggtgga attttatcgg ctctggttgg acagccgtct
     7201 gctgtactca tgcggctatt accaacatga ggaagacgac ctggagcagg cacagttcca
     7261 aaagctggaa tacctgtgcc ggaaattgcg gctcaacccc ggtgatcgga ttctggacat
     7321 cggctgcggc tggggaggct ttgcactcta tgcggctcaa actcacgggg cgctggtgca
     7381 tggaatcacg ttgagccgtc gccaagccga ggtggccaac catcggatca cggctgccgg
     7441 gatgtcccgg cgttgccgca tcgaagtacg cgactttcgg gacgtgcatg gcgatcaatt
     7501 gtacgacaag atcatcagca tcggcatggt ggagcatatc gggacggacc ggctggcgcc
     7561 ctattatgaa cgggctttgg gattactctc acccggtggc gtgttcctca atcaggggat
     7621 tggaaccaga gatggggaac cggccctcgg cgcctttgcc caacggtacg tctttccgga
     7681 cgcggagtta gtccctattc aggacatcgt gcgtacggct gaaagctgcg gcttcgaagt
     7741 gtgcgacgtc gagagtttgc ggcaacacta tgccctgacg ctggacgcct ggggacagcg
     7801 cctggaacgg catcatcaag aagccctgcg ggccgtaggc gaggtgacct atcgcgtctg
     7861 gcgggcttat atggccatgg ccgcctatct gttccgccaa gggcgtctca gcctctacca
     7921 tacgatgtgt gtgaaagccg tggacgggca tacggacctt ccgctcaccc ggaaggattg
     7981 gtacgaaagt cgcatctccg aagctcgagt tcgccaggaa gctgcctaac tgcctgcatc
     8041 acactcggcc gctcaaagct gcagagccaa ccgtccggtc tgcggtcggt gcacggtgtt
     8101 gccaagagcg aatggcgatc acgtttttcc atgcctaaac ctgccaccag aggaacccaa
     8161 tcggccggat ggcgacagct gctccgcgac cgaagcgtgc gatggctcta cgccggacaa
     8221 atcatttctc aagtaggcga cggcgtcagc aaagtggcgt tgctatggtt cgtgtatg
//
LOCUS       DASSCQ010000022         8259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1395794, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000022 DASSCQ010000000
VERSION     DASSCQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395794"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VFH05_01515"
     CDS             <1..916
                     /locus_tag="VFH05_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET6676923.1"
                     /translation="NDKLPSVSKPVKPVNDHRLYIPVSGLVHVVRKGKGFLVINQVDL
                     EEYVKGVVPGEVNSSWHPEMLKAQAVAARTYALYQQMLSAARDYDVVATVQDQVYKGK
                     DGVDTAVLRAVDETRGLVLTYQQAPIYAAFSSTAAGLTEDAVNVWSKEYPYLKGVECP
                     FDLESPFYQWKASFRMDKLEQNLRQQGYVVGTISSIEPLAFSRGGRVAKLRILHSEGE
                     LILRGEDLRKAVGYSIVPSTQFTIDSVGREVVLSGYGAGHAVGMCQWGAKELAELGYS
                     FSTILSYYYPGTELQEMSLTKPPIPPTS"
     gene            950..1987
                     /gene="queA"
                     /locus_tag="VFH05_01520"
     CDS             950..1987
                     /gene="queA"
                     /locus_tag="VFH05_01520"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET6676924.1"
                     /translation="MQLSDFDFPFDPSLVATEPTLPREQAKLLVLHPHQQAIMHRRIA
                     DLVDLLDPGDLIVVNDTRVRPARVRGRKPASGRPVEMLFVKPVADREWEVLMKGTWRA
                     GQAIEIDGESDVTVLSREGGRTVVRINGAWPIPEWFRVHGRMPLPPYIKRPPTDQDRE
                     WYQTVFARDEGAIAAPTAGLHFTPALLSQLTDRHIDLATITLHVGVGTFKPVTAECLE
                     EHRMDAEWVEVGTAAVRAIERAKAGGRKVVAVGTTVVRALETAALQGMLQPYRGETSL
                     FVRPGFQFKVVDALLTNFHLPQTTLLMLVSAFAGTGFLRRAYEEAVQTGYRFYSYGDA
                     MLVTDPWTRSS"
     gene            complement(1984..2682)
                     /locus_tag="VFH05_01525"
     CDS             complement(1984..2682)
                     /locus_tag="VFH05_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET6676925.1"
                     /translation="MIKILRFANEKYPWFLAITMGFIAVSFIVGMGWWGFGEQSGSVV
                     ATVGDLPISLDEFKRAYENTYRFYKEKVPGDFKDETIKQYVVEQLVENRVWLVAAHNM
                     GLTVADDELRESIMQIPEFQNNGKFDPERYQRLLAANHLTPSIFETMQARELLSVKAR
                     VLVSNAVSLTPSELAEAQALMLRQPDPDPGKAAAAKERTIQDVLFQKQQRALMAYAQS
                     LKATLPIAIHRELL"
     gene            2857..3312
                     /locus_tag="VFH05_01530"
     CDS             2857..3312
                     /locus_tag="VFH05_01530"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET6676926.1"
                     /translation="MAEQVAFHPLGAGIYPKATVLNRFIAKLIDVFLAAAAAEVIVPV
                     GLLAGVAYLFVADGFAGGRSIGKRLIGLQTVLPDSREPAGFRESIIRNIPFAAAQVLF
                     VVPYVGWLASTVIVAFEAILIIGNEQGRRLGDELAGTQVLDAGRLALPE"
     gene            3385..4434
                     /locus_tag="VFH05_01535"
     CDS             3385..4434
                     /locus_tag="VFH05_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET6676927.1"
                     /translation="MGFTSDVFGWFSNDLAIDLGTATTLVYVHGKGIVLNEPSVVAVE
                     KKTEKVLAVGADAKKMLGRTPGNIVAVRPMKEGVIADFEMAEQMLKRFIQKAHNRSAF
                     VRPRIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDVGGGTTDIAVISLGGIVYSESVKVAGDRMDDSIMNYIKKKYNLLIGEHMAERIKFE
                     IGSAYPFEERKTMMIKGRDLISGIPRTLVVDDAEIREALQEPIGTIVNAIKVALENTP
                     PELAGDIIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELD
                     LLAKVAVMSQANTFR"
     gene            4457..5332
                     /gene="mreC"
                     /locus_tag="VFH05_01540"
     CDS             4457..5332
                     /gene="mreC"
                     /locus_tag="VFH05_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET6676928.1"
                     /translation="MVNVRSSYGARRLALGLFALLLAALFLFPGQSQGLLDYLDGPVG
                     QLVRVPLEAVSSLDQGISTMWQQYVALQNVADDNRRLRQEMDWLRGENHQLREAAAAT
                     QRLTVLLQFKEQAAPTMVAAQVIGRDSSNRYRSMILNKGESDGIQKDMGVVTPSGVVG
                     RVVKTTGATSVVLLVTDPNNAIAGLIQRTRDEGIVEGTPQGRAKLKYIPMLSSVKEGD
                     QVVTSGLVGGFPRGLAIGRITAIDREEGALFQTAELAPEVDVNHLEEVLVIQTPHGES
                     AEAVRRPDGGTKRQP"
     gene            5329..5832
                     /locus_tag="VFH05_01545"
     CDS             5329..5832
                     /locus_tag="VFH05_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676929.1"
                     /translation="MKLLLYAMLVVFLVPLQATLLPHLAIWDVRPDLGLVVAALIGVL
                     AGELDGLLVGLAIGWVLNLYSAEDLWLNLLTTGGTGLFAGLLARHVAEVTPTVLSVAL
                     LVLSLAGGLVAVFGMKHGTISDTWWMVQSVVVPQACFDAAVGAALLWLLGQRFVMQRF
                     GVVDRYS"
     gene            5852..7720
                     /gene="mrdA"
                     /locus_tag="VFH05_01550"
     CDS             5852..7720
                     /gene="mrdA"
                     /locus_tag="VFH05_01550"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6676930.1"
                     /translation="MATSRLHDSELSELQRRLVVLRVGLLLVVALLALRLWHLQIREG
                     PYYRDLSENNRTRSVILEPARGLIYDRNGVLLANNVPSFSLYVSLEDVKDREVLIEQL
                     SELIGIDAELVRKKLAVRGAKQLPRKIKDRLTLKEATLIEAHRLDLPGVMVQVESRRN
                     YLGGVAASHVLGYVGEVSAEQLDKPEFAELHQGSIVGQYGVEKFFDQNVRGMAGQKNI
                     EVDALGHEKRTVLVDQPHAGDDLYLTIDARLQKVAEDLLGQESGAIVALDPTKGDILA
                     MASRPAFDPNVLSRELTPKQWVEIVQDEGRPLNNRASQGQYPPGSTFKVMMAAAALES
                     KTVTPASTVFCNGGYQFGRRVYHDWKATGHGSVDLRKALIHSCDVYFYTVGQRMGIDT
                     MASFAHQFGLGEETGIELPSERIGIVPSTEWKQKVKHEPWLPGETISAAIGQGYVTVT
                     PLQMASLIGTVANDGVTYRPRLVRAIMDRTTGQLQELPPVPKAKLNVKPETLKLIREA
                     LAGVVTEGTGGRAKSSIVTIGGKTGTAQTAALRTGPEKDIPKKFRDHAWFVAFAPVEN
                     PKIAVAVLAEHMGHGGSAAAPLAKEVIETFVKVTAKSGGAADGPLAQSQAVHAGAR"
     gene            7717..>8259
                     /locus_tag="VFH05_01555"
     CDS             7717..>8259
                     /locus_tag="VFH05_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET6676931.1"
                     /translation="MIDRMMDRRGLDNFDLRFLGLIFAILGLGVLSIYSVTHDQGGPG
                     LPFFAKQMVWILLGAVAFLVMLIWDYHRIARLAYPAYAIILILLAVVLFEGKTSRGAQ
                     RWIPIGPFAFQPSEFAKLVLILVMAHYYSKAPRVGWLQRVVIPGALMLPGLLLILKQP
                     DLGSGLSFMAVYAAMLLMVGM"
BASE COUNT         1674 a         2222 c         2572 g         1791 t
ORIGIN      
        1 gaacgacaag ctgccctcgg tgtcaaagcc ggtcaagcca gtgaacgatc atcgcctgta
       61 cattccggtg agcgggttgg tgcacgtcgt acgtaaaggc aagggctttt tggtcatcaa
      121 tcaggtggat ctcgaggaat atgtcaaagg agtggtcccc ggggaagtca attcttcctg
      181 gcatccggag atgttgaaag cacaggccgt ggctgcgcgt acctatgctc tgtatcagca
      241 gatgttgagc gccgcacggg actatgacgt cgtggcaacg gttcaggacc aagtctataa
      301 aggaaaggac ggcgtcgata cggcggtctt gcgtgcggtt gacgagaccc gtggtctcgt
      361 gttgacctat caacaagccc ccatttacgc cgccttttcc tcgaccgccg ccgggttgac
      421 ggaagatgcg gtgaacgtgt ggtccaagga atatccctat ctcaaaggcg tggaatgccc
      481 gttcgatctg gaatcgccct tctatcaatg gaaggcgtcg ttccggatgg ataaattaga
      541 gcagaatctg cgccagcaag gctatgtggt cgggacgatc tcatcgatcg agccgctggc
      601 cttcagtcgt ggcggccgtg ttgcgaagct gcgaatcctt cattcggaag gagagctcat
      661 tctgcgcggg gaggatctcc gcaaagcagt cggctattcg atcgtcccga gtacacagtt
      721 tacgattgat tccgtcggcc gggaggtcgt gctatcaggc tatggggcgg gccatgccgt
      781 tggcatgtgt cagtggggcg cgaaggagtt ggccgagctc ggctattcgt tctccaccat
      841 tttgtcctac tattaccccg gcaccgaatt gcaggaaatg tcccttacca agccaccgat
      901 cccgccgacg tcgtaagaca gtgcgcgcat gaacgctcac tagagatcga tgcagctgtc
      961 tgactttgac ttccccttcg atccctcgct tgtcgcgacc gaaccgacgc tcccgcgtga
     1021 acaggccaag ctcctcgtgc tgcacccaca ccagcaggcc atcatgcatc gccggattgc
     1081 cgatctcgtc gatctcctgg atcccggaga tctcatcgtg gtgaatgata cgcgagttcg
     1141 accagcacgt gtgaggggaa ggaaaccagc ctcaggacgg ccggtcgaga tgcttttcgt
     1201 gaagccagtg gccgatcgtg agtgggaagt cctgatgaag ggaacttggc gggccgggca
     1261 agccatcgag atcgacggag aatccgatgt caccgttctc tcgcgggagg gcggtcgaac
     1321 ggtcgtccgg atcaacggag cctggcccat ccccgagtgg tttcgggtac acggccgcat
     1381 gccattgcct ccttacatca agaggccgcc aaccgatcag gatcgggagt ggtaccaaac
     1441 cgtgttcgca cgggacgaag gggccatcgc tgcgcctacc gccggcctgc atttcactcc
     1501 ggctctcttg agccagttga cggatcgaca catcgatctg gcgacgatca ccctgcatgt
     1561 tggcgtaggg accttcaagc cagtcaccgc agagtgcctc gaagagcatc gcatggacgc
     1621 ggaatgggtg gaagtcggaa cggccgccgt gcgcgcaatc gagcgcgcga aagcgggtgg
     1681 acgaaaggtt gtggccgtcg gtaccacggt ggttcgtgcc cttgaaacag cggctctgca
     1741 agggatgctg cagccgtatc gaggagaaac gagtctgttc gtgagaccag gatttcagtt
     1801 caaggtggta gacgcgctct tgacgaattt ccatctgcct cagacgaccc tcctgatgtt
     1861 ggtctccgcg ttcgcaggca cggggtttct acgtcgcgcg tatgaggagg ccgtgcagac
     1921 cggctaccgc ttttacagct atggagatgc gatgttggtc acagacccgt ggacaaggtc
     1981 gtcttagagc agttcccggt ggatagcaat cggaagggtc gccttaagcg attgggcata
     2041 ggccatcagc gcgcgctgct gcttctgaaa cagcacgtct tgaatggttc gttcttttgc
     2101 cgccgcggct ttgcccggat cgggatccgg ttgtctgagc atgagggctt gtgcttccgc
     2161 gagctcggac ggcgtcaagg agacggcgtt actgacgagt acgcgggcct tcacgctcaa
     2221 cagctctctt gcctgcattg tctcaaagat tgagggcgtc aaatggttgg ctgccagcaa
     2281 tcgttggtag cgttcaggat caaatttgcc gttgttctgg aactcaggaa tttgcatgat
     2341 cgactcgcgc agctcgtcat cggccaccgt caaacccatg ttgtgcgcgg cgaccagcca
     2401 cacccgattc tccaccaatt gctcgaccac atattgcttg atcgtttcgt ccttaaagtc
     2461 tcccggaacc ttttccttgt aaaagcgata ggtgttttca tatgcgcgtt tgaattcatc
     2521 caatgagatg gggagatcgc ccaccgttgc gacgaccgag ccggattgct cgccgaaacc
     2581 ccaccagccc atgcccacga tgaacgacac ggcgatgaat cccatcgtga tcgccaaaaa
     2641 ccacggatat ttttcgttcg cgaaccgtaa gatcttgatc atggaccggg atccctcccg
     2701 acaagtgaaa tagtcgcgga ttgtacccag cctcctgtgc cgaaggcaag tttctcagtg
     2761 cccatgggcc tcgccgtcgt ggcgtcaaag aatggtgatt cgtttgtgct aggtcctggt
     2821 ctttgttata ctttgcccct aattacgagg agatccttgg cagagcaggt ggcgttccat
     2881 ccattgggtg cagggatcta cccgaaggcg acggttctca atcgattcat cgcgaagctc
     2941 attgacgttt ttctcgcggc agctgccgcc gaagtcattg tgcctgtcgg tttgctcgca
     3001 ggagtcgcct atctcttcgt ggccgatgga tttgcggggg gtcggagcat cggtaagcgg
     3061 ctgatcgggc ttcagacggt tcttcctgat agccgtgaac cggcaggatt tcgagagtcc
     3121 atcattcgca atattccttt cgcggcggcg caggtattgt ttgtcgtccc ttatgtcggc
     3181 tggctagcct cgacggtgat tgtcgcgttc gaagcgatct tgatcattgg aaacgagcag
     3241 ggccgccggc tcggtgatga actggcgggg acccaggtgt tggacgcggg ccgattggca
     3301 ttgcccgagt aggaacaggc gcatgtgagg ctggcgtgag ccgcgtcatc caggattcgg
     3361 tttgcatgtg aagggagagt gaccgtgggt tttacgagtg acgtgttcgg ctggttctcg
     3421 aacgacctgg ccatcgacct gggcacagcc acgacgttgg tgtacgtgca cggaaagggc
     3481 atcgtcttga acgaaccttc cgttgttgcc gtcgagaaga agacggaaaa agttctcgcc
     3541 gttggcgccg atgcgaagaa gatgctcggc cgcaccccgg gaaacattgt ggccgtacgt
     3601 ccgatgaagg aaggcgtcat cgcggatttt gaaatggccg aacagatgtt gaagcggttc
     3661 attcaaaaag cgcacaatcg cagcgccttt gtccggcccc gcatcattat cggcgtcccg
     3721 tcacggatca cccaggtgga gcagcgagcg gtgcgcgatt cggccgaact ggccggcgcg
     3781 agggaagtgt atttgatcga agaaccggtc gcggcggcga tcggcgcggg cctccccatc
     3841 accgaacctt ccggtaacat ggtcgtggac gtgggcggag ggacgacgga catcgcagtc
     3901 atttcgctgg gtggcatcgt ctacagcgag tcagtgaaag tcgccggtga ccggatggac
     3961 gactcgatca tgaactacat caaaaagaag tataacctcc tcatcggcga gcacatggcg
     4021 gaacggatca agttcgaaat cggctccgcc tatccgttcg aggagcgcaa aacgatgatg
     4081 attaaagggc gcgacctgat ttccggcatc ccgcgcacat tggtggtgga tgatgcggaa
     4141 atcagagagg cgctgcagga gccgatcgga acgatcgtca atgcgatcaa ggtggcgctg
     4201 gagaacaccc cgcctgagtt ggcgggcgac atcatcgacc gtggcatcgt gctgaccggc
     4261 ggcgggtctt tgctcaaggg catggatacc cgcttccggg aagagacgaa tctgccgatc
     4321 atcaccgtcg acgatcccct gacttccgtc gtgttgggag tgggcaagat cctcgatgag
     4381 ttggatctat tggccaaggt cgccgtcatg tctcaagcga ataccttccg gtaaggctcc
     4441 atcccggtac atgtcgatgg tcaacgtgcg gtcttcctac ggtgctaggc gtctggcgct
     4501 aggattgttt gccctcctcc tggcagctct cttcctcttt cccggacaaa gtcagggcct
     4561 tctcgattac ctggacgggc cggtcggcca actcgtccgg gtgcctcttg aagccgtgtc
     4621 ttctctcgat caaggcattt cgacgatgtg gcaacagtat gttgcactcc aaaatgtggc
     4681 tgatgacaat cgccgattgc ggcaggagat ggattggttg cggggagaaa accatcaatt
     4741 acgtgaggcg gctgctgcaa cgcagcggct cacggttttg ctgcagttca aggaacaggc
     4801 cgcacccacc atggttgccg cccaggtgat tggtcgtgat tccagcaacc gctatcgatc
     4861 gatgattttg aacaagggag aaagcgatgg catccagaaa gatatgggag tggtgacacc
     4921 ttccggtgtc gtagggcggg tcgtcaagac gacgggtgcc acgtcggtcg tcttgcttgt
     4981 gaccgatccc aacaatgcca tcgccggatt gatccagcgc accagagacg aaggcattgt
     5041 cgaaggaacg ccgcaaggac gggccaagct gaaatacatt cccatgctgt cgtcggtgaa
     5101 ggaaggagat caggtcgtca cctccggcct ggttggaggc tttccacgcg ggttggcgat
     5161 cgggaggatt actgcgatcg atcgtgaaga aggcgcactg tttcagacgg ccgaactcgc
     5221 cccggaagtc gacgtcaacc atctggaaga agtattggtg attcaaacgc cccacggcga
     5281 gtccgcggag gctgtccgga gaccggatgg cggaacgaaa cggcaaccat gaagctcttg
     5341 ctctacgcta tgttggtcgt gttcctcgtg ccgctacagg cgacgttgct tccccacttg
     5401 gccatatggg acgtcaggcc ggatctcggt cttgtggtcg cggccctcat cggagtatta
     5461 gccggcgaat tggatggatt gctcgtgggt ttggccattg ggtgggtgct aaatttgtat
     5521 tctgcggagg atctctggtt gaatctgctc acgacaggag gcaccggatt gtttgcgggg
     5581 ctcttggcaa gacacgtggc tgaggtgacc ccgaccgtgc tttcagtggc tctgttggtc
     5641 ttgtcgttgg ccggaggcct cgtcgccgtc ttcggcatga agcacgggac gatatccgat
     5701 acctggtgga tggtgcagtc ggtcgtcgtt ccccaagcct gctttgatgc ggccgtgggc
     5761 gccgcgcttc tgtggttgtt ggggcagcga ttcgtgatgc aacggtttgg agtcgtggac
     5821 cggtactcat aaagacgctg gaagaccaac catggcgacc tcccggctac acgacagcga
     5881 attgagcgaa ttgcagcggc gtttagtagt gctccgcgtg gggctcctac tcgtcgtggc
     5941 cttattggca ttgcgcttgt ggcatttgca gatccgggaa gggccgtact accgcgacct
     6001 ctctgaaaac aaccgcacgc gttcggtgat tctggagcct gcgagagggc tgatttatga
     6061 ccgcaacggt gtcttgctgg cgaacaacgt gccgagcttc agtctgtatg tgtcgctgga
     6121 agacgtcaaa gaccgagaag tgctgatcga acagctgagc gagttgatcg gcatcgacgc
     6181 ggagctcgtc cgaaaaaaac tggcggtgcg tggcgccaag caactgcctc gcaagatcaa
     6241 ggaccggctg acgctcaagg aagcgacctt gatcgaagcc caccgcctcg atttgcccgg
     6301 tgtgatggtc caggtcgaat cccgccggaa ttatttgggc ggcgtggcgg cgtcacacgt
     6361 attggggtat gtcggggaag tgtcagccga acagttggac aagccggaat ttgccgaact
     6421 gcatcagggc agcatcgtcg gacaatatgg tgtcgaaaaa ttcttcgatc agaacgtgcg
     6481 gggcatggca ggccagaaga atattgaagt cgatgcgttg ggtcacgaga agcgaacggt
     6541 actcgtggat cagcctcatg cgggcgacga tctgtatctg acgatcgacg cgcgtttgca
     6601 aaaagtggcg gaagatctgc tgggacagga atccggagcc attgtcgcat tggacccgac
     6661 gaagggggac atcctggcga tggccagccg gccggccttt gatccgaacg tgctgtcgcg
     6721 cgagttgact ccgaagcaat gggttgaaat cgtccaggac gaggggcggc cgttgaacaa
     6781 cagagcgtca caaggccaat atcctcctgg ttcaaccttc aaggtcatga tggcggccgc
     6841 cgccctcgaa tccaagacgg tgacgcccgc gtcgactgta ttctgtaatg gagggtatca
     6901 gttcggtcgg cgggtctacc acgattggaa ggcgacagga cacggctcgg tcgacctacg
     6961 gaaagccctc atccattcgt gcgacgttta cttttacact gtcgggcagc gcatgggcat
     7021 cgatacgatg gcttcgtttg cccatcagtt cggtctggga gaggagaccg gtatcgaact
     7081 gccgtccgaa cgcattggca tcgtaccctc gacggaatgg aagcaaaagg tgaaacatga
     7141 gccatggctt cccggcgaaa cgatttcggc ggcgatcggc caggggtacg tcaccgtgac
     7201 gccgctgcag atggcgagct tgatcggcac ggtggccaat gacggcgtga cctatcgccc
     7261 ccgcttggtt cgcgcgatca tggatcgaac gacgggacag ttgcaggaac ttccgccggt
     7321 accgaaagcc aaattgaacg tgaagccgga gacgttaaaa ctgatccggg aagccctcgc
     7381 gggagtcgtg accgagggaa ccggcggaag agctaagtct tcgatcgtca ccatcggagg
     7441 aaagaccggc accgcgcaga cggctgctct gcgcacgggc cctgaaaagg acatccctaa
     7501 aaagtttcgc gaccatgcct ggttcgtggc gtttgccccc gtcgagaatc cgaaaattgc
     7561 ggttgctgta ttggccgagc acatgggaca cggcgggtcc gccgctgccc cgctcgccaa
     7621 ggaagtcatc gagaccttcg tcaaggtaac cgcgaaatcc ggcggggcag ccgatggacc
     7681 tctggcccaa tctcaagcgg ttcatgcagg cgcacgatga ttgaccggat gatggaccgg
     7741 cgtggactgg acaacttcga tctgcgcttt ctcggcttga tcttcgccat cctcggactt
     7801 ggggtgcttt ccatctacag cgtcacgcat gatcagggcg gccccggatt gccgtttttt
     7861 gccaaacaga tggtgtggat actgctgggg gccgtcgcat ttttggtgat gctgatctgg
     7921 gactatcacc gcattgcacg attagcgtat cctgcctatg cgatcatttt aatcctgttg
     7981 gcggtcgtcc ttttcgaagg aaagactagc cggggagcac agcgatggat tccgatcggg
     8041 ccttttgcct ttcagccgtc ggagtttgca aagctggtgc tgatcctcgt catggcgcat
     8101 tactattcga aagcgcctcg agtcggctgg ctgcaacggg tggtgattcc cggcgcgttg
     8161 atgctcccgg gactgttgct gattttgaag caacccgatc tcggcagcgg attgagtttc
     8221 atggccgtgt atgccgcgat gctgctcatg gtggggatg
//
LOCUS       DASSCQ010000023        22188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_325923, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000023 DASSCQ010000000
VERSION     DASSCQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 22188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325923"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            546..1076
                     /locus_tag="VFH05_01560"
     CDS             546..1076
                     /locus_tag="VFH05_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET6676932.1"
                     /translation="MQEQQFVLDVKTIRDRARRQIENGAMTPGYKAKAPVVLRLLNDA
                     LATEIVCTLRYKRHYFMAKGLSSESVKSEFLEHAEEEQAHADAIAERIVQLGGEPNLS
                     PEGILDRSHSQYAEGTSLEDMIKEDLVAERIAIDSYRQMIEYIGGDDPTTRRLLEEVT
                     AKEEEHAEDLASLLAK"
     gene            1163..3340
                     /gene="glgX"
                     /locus_tag="VFH05_01565"
     CDS             1163..3340
                     /gene="glgX"
                     /locus_tag="VFH05_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET6676933.1"
                     /translation="MPRVTTRPGHPYPLGATWDGEGVNFALFSENATGVELCLFDRPD
                     QEKESHNIRIEERTNQIWHVFLPEVRPGQLYGYRVNGPYEPQAGHRFNANKLLIDPYA
                     KALHGSLRWSDSMFGYSIGDPDADLSFDERNNASLVPKSVVVEQAFTWGDDKPLRRPW
                     SETVIYEMHVRGFTVKHPLIHEALRGTYAGLATRPVIEYLQRLGVTAIELLPVHAFVA
                     DKHLIDKGLTNYWGYNTIGFFAPELRYAAHPEHAVNQFKTMVKTLHSAGIEVILDVVY
                     NHTAEGNHLGPTLSFRGIDNAAYYRLVPDQPRYYVDYTGTGNTLNVRHQRTLQLIMDS
                     LRYWALEMHVDGFRFDLASALARELHDVDRLGGFLDIIQQDPVLSQLKLIAEPWDIGE
                     GGYQVGNFPAGWAEWNGKYRDTIRRYWKGDGGQASELAYRLSGSSDLYEGSGRRPFAS
                     INFVVAHDGFTLRDLVSYDQKHNEANKEDNKDGSDDNQSWNCGVEGPTDDPAIRELRA
                     RQQRNMLATLLLSQGVPMICGGDEIGRTQNGNNNAYCQDNDVSWFNWDISSEEQALFT
                     FTSGLIMLRAAHPVFRRRQFFQGRSIYGADIKDLSWFRPDGEEMTEEDWKQGYVRCLG
                     VRLAGDRIEEKDHLGNPIFDDTFLLLINAHHEPIQFTLPPHPINLQWQLVLDTVQEVI
                     PAHAPLISEGKYYDLKGRSLALLQIETAVPIRPPLRGSPSAGS"
     gene            3377..3721
                     /locus_tag="VFH05_01570"
     CDS             3377..3721
                     /locus_tag="VFH05_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6676934.1"
                     /translation="MTRSVRASVFAITWGTAVAVVPTNALPQPAAEKPSDVQLQTAIV
                     RAMQTSPLTAGSLIVVHVNQGEVTLEGAAATPLARREATRLAETVLGVKGIRNRLSVV
                     NTQTAEVPAEQK"
     gene            3870..4100
                     /locus_tag="VFH05_01575"
     CDS             3870..4100
                     /locus_tag="VFH05_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET6676935.1"
                     /translation="MNADIFQGKWGQFKGELKRQWGKFTDDDLTQIEGNYDKFLGKVQ
                     ERYGAQKDDISGWADKWFQGNKPEQVEREPVR"
     gene            4123..4404
                     /locus_tag="VFH05_01580"
     CDS             4123..4404
                     /locus_tag="VFH05_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET6676936.1"
                     /translation="MLRVVICSIMVGVLAAGPAWALFESNKELVAGAKIPLEEAVKSA
                     IKAVPGKAAEADLGKDDGRTIWKIEVIDHNNKSQTVYVDAQTGMAKMNK"
     gene            4504..5094
                     /locus_tag="VFH05_01585"
     CDS             4504..5094
                     /locus_tag="VFH05_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6676937.1"
                     /translation="MRPLHRPVRSACLFGFVVWAMMIAVPAAGAEGLFEQVNIGPVSI
                     GGRATYYDPKDASGEWYGGAQVRLYAFRFLAFEGSADYRRNDFGNTRVHSYPVQVSAL
                     IYPFGTMRLAPFILGGGGWYYSTVKGPGNFDDTQNRFGFHAGGGLQFFLNKHWSIDST
                     YRYIWLERIESKDQNVVDKKFNDNGHMITIGLNFHF"
     gene            5161..6258
                     /locus_tag="VFH05_01590"
     CDS             5161..6258
                     /locus_tag="VFH05_01590"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HET6676938.1"
                     /translation="MGATSGVASFLCAVVLCALCGTVPAWASDEFIAGYASAILQHEF
                     NVTDAAVEVHDGSVLVTTKTLTNIDRGKVVAAIKQVPGVTQVEIRPADPSTISPEDQA
                     AHASIPEPGAKWLPRGTLVAPLHADPRWPQFGASYRQFTQGLNLAGVFAANFGETFAI
                     YRNKAFMDGEWEFGVQAGVFSIFDVSAQSIDLVNADYRVGFLSSYRNGPFSGFIRLQH
                     QSSHLGDEFLLNNPGITRVNLSYEELDVKLSYQVFQWLRVYGGGGFIVHRFPEDLGRG
                     TSQWGAELVSTRTFLGGRLRPVAYADFQCNERTRWQVGHSIMAGVQFENARIGDRQIQ
                     LLGEYYSGPSPDGQFYLQRAAWYGIGIHLYF"
     gene            6429..6644
                     /locus_tag="VFH05_01595"
     CDS             6429..6644
                     /locus_tag="VFH05_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676939.1"
                     /translation="MCASCWRVQHLSEEFGAELQEWSDPTTFMASGQVSASKYDIVDG
                     YCDPCLIEIALRDQLALARAEGERLNA"
     gene            6637..7365
                     /locus_tag="VFH05_01600"
     CDS             6637..7365
                     /locus_tag="VFH05_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676940.1"
                     /translation="MPENSGMHWLTGKRVLLAVLFALAFPWPLPSQAQDTLWNTWRQG
                     GLQALHEGRLADAERLLTAALEQAEKYGLDDLRVADAANDLAVVYANSGKPLEAELLF
                     GRALTIGEKGLGADHPGVGATIQNLGILYATQERYREAEPLLKRSLAINLKEFGTNHA
                     RTALSLKTLSSFYAIQGQLGEAERLIHKSLAILEESRVRQGDPQLISTLEVLAAILRN
                     TNREREAQEIEGQLKTAQDTTVYR"
     gene            7450..7617
                     /locus_tag="VFH05_01605"
     CDS             7450..7617
                     /locus_tag="VFH05_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET6676941.1"
                     /translation="MLSWAVTFLVVAIIAGILGLSGVAGTATQIAYVLFVVFLIFAAV
                     SFVMGRRPPVT"
     gene            7693..8076
                     /locus_tag="VFH05_01610"
     CDS             7693..8076
                     /locus_tag="VFH05_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676942.1"
                     /translation="MKLLPSLVLGVWTLLSHSAAESAMQEAKHFVVDIISINGEEFVV
                     KDENGKEANIHVGTETEKFGQLQVGDRVDAWVYPNGQARTLMILRSASVIKQDQSQQN
                     QQEAQQKQQRAEAPQEVHPGEAAIK"
     gene            complement(8208..11492)
                     /locus_tag="VFH05_01615"
     CDS             complement(8208..11492)
                     /locus_tag="VFH05_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6676943.1"
                     /translation="MANYTSYDELLRENAALKERLAEQERLGEQSRQAEEQGRIAEHF
                     RRVAAETALRESETRYRALADTMPQVVFIATASGFVEYVNRPGLEYIGLRLDEAIGFG
                     WLGAIHPDDRQLVSERWLECLRIGKTYEFENRLLRADGHYRWQLARAVPIVGESGAIV
                     RWIGTSTDIHDHRQAQQDLQQSRTEAEYQAEELNRLYQTSPAGLAFLDTELRFVRINQ
                     TLAQINGFPPEAYIGRTLREMLSPAMADRLEPVLRLVVDEAKAAMNVEFEGEPPSDPG
                     NIRYWLANYYPLVNREGSVLGVNVVMQDMTERKRTEEALQRSHAFIRQIIDTDPNFIF
                     VKDRDGRFTLVNQAVADCYGTTVEELLGKTDADFNSNVEEVDQFRRKDREVIDGLKEL
                     FIPEEVITDASGSVRWLQTVKRPILGERGQATHILGVATDITQRRLAEEALRRNEELL
                     RLVTDSSPIAMAYCDEQQRLRFVNRAYVDRFRLEPSQVIDKPIAEVIGSQAYDTFRAH
                     IDAALRGTRTEFEAAVPYERIGTRYMRCAYVPHLDHQGRAIGFTAVMMDITDRRRMEE
                     ALRESEERLRLALAAGQMGAWDVDLGTNVTRWDRKEFALFGLLEGSITPSTPEFYRHV
                     HPDDLPLVEQSVQRALSGSTGALEHEFRVIRPDGAIRWLAAKGQVLNDHEGRPVRMVG
                     VNFDVTERRRTEERLRSFTHELEARVAERTQELTQLHERLRALATELNLTEQRERKRL
                     ATDLHDYLTQLLALVCMKLSQIKRKAPGPTQAEIAQTEGVVNEALVYTRTLVTQLSPP
                     VLHEFGLPVALRWLAEQMIRQELTVEVRQSVPDQVSLPEDRAVLLFQSVRELLINVRK
                     HARTNHALMTIEQTGASLCITVRDEGAGMDLQAAQSSQPSAMSSKFGLFSIRERMLAI
                     GGCLEIESAPGRGMTSRLTLPLTRPESMDAGKLQTPCQSQEDGTLQHDERTSAFKDGR
                     IRVLLVDDHTIVREELRVLLYDHQDLLVVGEAGDGEQAVLLADKLRPDCVIMDVNMPR
                     MDGMEATRRIKAAFADTAVIGISVNNSQEMVAAMRRAGADAFLSKEAPAEDIHHTIVT
                     MTRKCA"
     gene            complement(11543..14467)
                     /locus_tag="VFH05_01620"
     CDS             complement(11543..14467)
                     /locus_tag="VFH05_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6676944.1"
                     /translation="MTEEMTTIVNVDDQEAGRYARRRMLERAGYRVIDATCGADAWRV
                     VEEVRPPLVLMDVNLPDISGFDLCRRIKNRPELADIMVLHVSASRVSGMDRAFGLEVG
                     SDGYLIEPIDEKELLAAVRSLLRLRERQQENEGLLARLRRSEELFRGLFEQAAVGMCV
                     VGSDGRLLRVNDKLCSITGRAEHDLMKQELRDLTHPEDRAEHESQVGTLLLGGISEVS
                     LEQRLMRPDGETIWVTVTLSIQWDAGGRIQSLIGVIEDIDARKRAELELRTHTARQQL
                     LSKTMDQLLRVDDPRDVMQDIFESLRKSVGLDTFLDYDVMPDNSGIRMATWAGIPDPA
                     LEPLMFLQFGQALCGTSAVSRQPVIVDHLQSSSDPKASRMQAVGSTAYACFPLIVGDR
                     LLGTLAFASNTRDSFEPDDLELLEAVSNYVAMAKERRRLLHETRQRAEQVAEQEERLR
                     FATEGAGMGTWDVDLRTGRVVWNRQNALLLGYDFESGQASAKRWWALVHEEDRAIVRQ
                     AMKNASKSGQLFQAEHRIRRADTGELRWLAPYGRIHYNGGGRPTRFVGVTFDITERKQ
                     SDIQRQEWTRELERRVNERTKELVRTHDRLRGLAQDLTLTEQRERQRIAGELHDYLAQ
                     MLVVTRLKMNQATQMISPHAACSVLQEADQLIDQSLTYTRTVVAELMPPTLRQFGLAA
                     GLRWLGERMGQQGLDVSVDVPDVAPPLSEDHTTMLYQCVRELLFNVLKHAGTDRASVT
                     MGDDGQGKLHIVVHDEGVGCDPVTIRESKGDKFGLFSIRERMEAIDGWMELDSQPGAG
                     TTVTLGVTVGASGAEDATADHLPLNAAQPSGASGFSVHHPAHERQAPVRVLLVDDHAM
                     VRQGLRSILDGYANLHIIAEAGDGEEAITLARLLKPDLIVMDVNMPRLDGIEATKRIR
                     EELPGTYVVGLSVNEAEHVVKSMRDAGASAFLTKESAADHLYETIGMVIRKTSVECSM
                     DA"
     gene            complement(14691..15371)
                     /locus_tag="VFH05_01625"
     CDS             complement(14691..15371)
                     /locus_tag="VFH05_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676945.1"
                     /translation="MHYRTLAIIWASIVPLLASCTLPREITKTPRSAIEQLLLTQALN
                     RSLLDLRLPVSPAEPLYMKVSGLQLGYMSPLLPLATGSMGSASTSTATPPTEGYFSPA
                     GDLAFISDAVASHLGVLGYRMSRREDEAVYVVRVVVQSFGTIQATSFFGMPPIQSVLI
                     PFSLPQLTVYQNLSQDGYIKYGVEVVERASGRLVYTTPWHSHRTYHDQYTILFFFSFR
                     MTDLDEAP"
     gene            complement(15383..15742)
                     /locus_tag="VFH05_01630"
     CDS             complement(15383..15742)
                     /locus_tag="VFH05_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676946.1"
                     /translation="MRNIIAYGLTILRGSLYVILLVLVTSPLTGRSEETVPVEQPDAQ
                     LRVTGKLAKLDIEAGKGMLLTDLEKPIFFRLDRPDLLERFSIGDRVTMQLDEEGRVLK
                     VIETLPAEVHEPPPPAK"
     gene            16039..16590
                     /locus_tag="VFH05_01635"
     CDS             16039..16590
                     /locus_tag="VFH05_01635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET6676947.1"
                     /translation="MLDPLLRVSSRLITAARHSEYATARPVFEWEIEVCEDETVVQPF
                     VHPEGSIIVCSGTFRVAETEAGLAALLSHELAHALKHCASPAPELCHRPDRHNETLFT
                     IDQEIEADGIGMNLMADAGYDPSELLLIWKRMKESHRERGDALLVHVTYDRRMDHIKG
                     KLPDALRLYAETHRAPQRPLPVR"
     gene            complement(16667..16915)
                     /locus_tag="VFH05_01640"
     CDS             complement(16667..16915)
                     /locus_tag="VFH05_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676948.1"
                     /translation="MNQLVPTSTFTPEYAGLPSEIAGKIVQMVKHRQACDMEDLLEAC
                     STYTWNQVFLEVDRLSRTGELRLFSNRAGTYTVTLPAA"
     gene            17170..18345
                     /locus_tag="VFH05_01645"
     CDS             17170..18345
                     /locus_tag="VFH05_01645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6676949.1"
                     /translation="MNSAQPQAKSNELDLEDQPRVHVGCGLCGSRRSRLVCSPEDIVV
                     QQRFLEHFYRSRWSQHNAATATDRVSFTQDYATSIVACMDCGLLYRNPRPRAEAVTQA
                     YETEDYDEAYLQAEFTAQQTWARGKADILARHLEKIGRTGRPRVLEIGSFVGGFLLEG
                     QLRGWDMLGVDPGHDVASFCRRHRLPIFEGTLEDAQLPPASFDAIAIWNTFDQLPDPR
                     KLLHQLLPLMRNGGLLVARVPNGACFEWGMSMRARLPKVFRRPLDIALACNNLLTFPY
                     LYGYSAKQLEELTEPFGFRLLACYADQIVSSPPGHLRPWALWEERVVKTLCKAFAVLW
                     PDNRSLRFRSAPWLDCIFERACTDEERPAPQKLGLGVIPVYSPLVFKDTGLDCSVNR"
     gene            18929..20527
                     /locus_tag="VFH05_01650"
     CDS             18929..20527
                     /locus_tag="VFH05_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6676950.1"
                     /translation="MIDEYPGQAPDKRARLLVTIVLLFTVTVATFAADRLLPLGVAVW
                     MLYLVPVVLTTQLLPQYTGLALAISSGLLLFGVLDSKEGISYELAVINRGLGLIVLLF
                     TGLFLLQHRRMAEALRESEGRYRLLLEQHGRARAEAALHEVQERFTSIFRSSHDAIAY
                     ATFDGRLLEVNDAFVRVSGRPREALLSPEPPTSLWPSLGLSPEQFQKLRETGKPLVYE
                     TMSRQDGPGAEWLNVHAFAVRGSDGAISGLAVVIRNISDRKQAEAELRSTSEELRAKN
                     EALARTNELLSAAQHAAARAQRLSAIGQFAATVAHKIGTPLTALSGHVQLLMEDLSLP
                     SKVRGRLQTVEAQIERTSRIIQDLLLYTRRAPPVRTKIDINECLRECASLFRTECERQ
                     HVACITELTPDLPPVEADRQHMLEALNHLIENALQAMPNGGSLCLRTASIEPVTVGRG
                     LHHTGGVAIEIADTGHGIKPDHSAQIFQPFFTTKQAGRGTGLGLAIVQETVRAHDGRI
                     TVESEPGKGTTFTIHLPAVDKGTP"
     gene            20527..21882
                     /locus_tag="VFH05_01655"
     CDS             20527..21882
                     /locus_tag="VFH05_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6676951.1"
                     /translation="MDDPANILVVDDDEETVTLLREILSKEGYNVDTAADGKIALDKI
                     GSRPPDLVISDIHMPDLDGMSLLAELRVRFPDVPVILMTAYGSLKTAVDGLRSGAFDY
                     LSKPFIVDDIRLVVRRALEHKNLSRQNQALKEQLRHRYRFDNLVGSSPGIVSVYKSIA
                     RVANTESTVLLQGESGTGKELIARAIHANSARSAGPFVAVDGGSLAETLLESELFGHE
                     RGSFTGAVTSKKGLLEKAHLGTCFLDEVADLSPVLQGKLLRVIQEREIRRVGSTTPMT
                     IDVRMVAASKKDLEQLVKAGTFREDLYYRLNVVTINIPPLRERAEDIPLLTQYFIQMY
                     GGKKSPPVTGISPEAMALLTRYWWPGNIRELEHAIERALALTPHPVIFPEDLPQSIQS
                     ATVQAAAQARGWVTLAELEKDHILRVLEAHNQDLGRAATILGIHRKTLLRKLRYFGFP
                     C"
BASE COUNT         4871 a         6302 c         6227 g         4788 t
ORIGIN      
        1 aatcaaggag ctggcggata ctctccaaaa cgaggggatg atcatcgact aacagtactt
       61 tggcggtgtg ggtgtgtagt gacattatcc tctgcgtccc gtgtgacgaa aacttcccag
      121 ccccctatca tggtacaaat cagcccctcg gttcaagcaa ataaaccgtt gatttgtttt
      181 accgcaattg gcgaaaagga agaactgggc catcccctag cgctaagcgg gaaaccctag
      241 gggtgctccg agtgtgcgca acgccctact ggctttaggt tattttgagg atgtgcccat
      301 tcggttgagc gcctttttat gacgcttcta ccgcgtatga cgcttctacc gcgtgcctgg
      361 accaagttcg tcgttcagcg gatacattga tgaatcggtg ccgcctggca ctcaccacac
      421 tacggcaccg ccgcagagta aggtaggctg agttgtgcgt cccctcctct cgcgaaatcc
      481 acatctcatt catctaaaaa gagactcaaa gaaacactcg aaaccgacgc cacaaggagg
      541 atgtcatgca agagcaacaa ttcgtgctcg atgtcaaaac cattcgtgac agggcacgcc
      601 gccaaatcga gaacggcgcc atgacgccag gctataaagc caaagcgccg gtcgtgctcc
      661 ggctgcttaa tgacgcgctc gcgacagaaa ttgtctgtac gcttcgatac aagcgacact
      721 attttatggc taaaggtctc agttcagaaa gtgtcaaaag cgaatttctc gaacatgccg
      781 aagaagaaca agcccatgcc gatgccatcg cggagcgcat cgtacagtta ggcggcgagc
      841 cgaatctctc ccccgaaggc attctggatc ggagtcactc gcaatacgcc gaagggacat
      901 cgttagagga catgattaag gaagatctgg ttgcggaacg gattgcgatc gacagttatc
      961 gacagatgat cgaatacatc ggcggtgacg atccaactac ccgcaggctt cttgaagaag
     1021 tgactgccaa ggaagaggaa catgcggagg atctcgccag tctccttgca aaatgataag
     1081 ccgaccaggt tacaacccgg agcgtgaggg tcttttctta tgaatcattt gcctctgtaa
     1141 accgtcaaag aaggagtggg ctatgccgcg tgtcactact cgtcccggtc atccatatcc
     1201 cttaggcgcg acatgggacg gtgaaggtgt caacttcgcc ttgttctcgg aaaacgccac
     1261 cggagtggaa ctttgtctgt ttgaccgccc ggaccaggaa aaagaaagtc ataacatccg
     1321 gatagaggag cgaaccaatc agatatggca tgtattcctc cctgaagtcc ggcccgggca
     1381 actttacggc tatcgggtca acggtcctta tgaaccgcaa gctggccacc gcttcaacgc
     1441 gaacaagttg ttgatcgatc cctatgccaa agccttgcat ggatctctac gatggtctga
     1501 ttcgatgttt ggctacagca tcggagaccc ggacgccgac ctctccttcg acgagcggaa
     1561 caatgcttcg ctggtaccga agtcagtggt ggttgagcag gcgttcacgt ggggagacga
     1621 taagcctctg cgacgtccat ggagcgaaac ggtcatttat gaaatgcacg tgcggggatt
     1681 caccgtcaaa catccgctga tccatgaggc actccgcggc acctatgccg gtttggccac
     1741 gcgtccggtg atcgaatatc ttcaacgtct cggcgtgacc gccatcgaac tgctccctgt
     1801 gcatgccttt gtggcagaca agcatttgat tgacaaaggc ttgaccaact attggggcta
     1861 taacaccatc ggcttttttg cgccggagtt gcgctatgca gcccatcctg aacatgccgt
     1921 caatcaattc aaaaccatgg tcaagacgtt gcacagcgcg ggaatcgagg tgattctcga
     1981 tgtggtgtac aaccataccg ctgaagggaa ccacctgggg cccaccttat cgtttcgagg
     2041 cattgataac gcggcctact atcgtctcgt gccggatcaa ccgcgttact acgtcgacta
     2101 cacgggcaca ggcaacacgc tcaacgttcg tcatcaacgc acactgcagc tcattatgga
     2161 cagcttgcgg tactgggccc tcgagatgca tgtcgacgga tttcgcttcg atctggcttc
     2221 cgctctcgct cgtgagctac acgatgtcga ccgcctcggc ggcttcctgg acatcattca
     2281 acaagaccct gttctctcgc aactcaagct catcgccgaa ccgtgggaca ttggcgaagg
     2341 cggatatcag gtgggaaatt tcccagccgg ctgggcggaa tggaacggca aataccggga
     2401 caccatccgg cggtattgga aaggcgacgg cggtcaagcc agcgagttgg cctatcgcct
     2461 gtcaggcagc agcgacctct atgaaggcag cggacgccgt ccattcgcga gcatcaactt
     2521 tgtcgtcgcc catgatggct tcacgttgcg tgacctcgtc tcgtacgatc agaagcacaa
     2581 tgaagcgaat aaggaagata acaaggacgg atcggacgat aaccagagtt ggaactgcgg
     2641 ggtcgagggt ccgacggacg atccggccat ccgcgaattg cgggcgaggc aacaacggaa
     2701 catgctcgca accttattgc tctcgcaagg cgttcccatg atctgcggcg gcgacgagat
     2761 tggccgaacc caaaatggga ataataacgc ctattgtcag gataacgacg tgagctggtt
     2821 caattgggac atctcgagcg aagagcaggc gctctttacg tttaccagcg ggttgatcat
     2881 gcttcgagcg gcgcatcccg tctttaggcg ccgacaattt ttccaggggc gaagcattta
     2941 cggtgccgac atcaaggacc tgtcctggtt tcggccggat ggcgaggaaa tgaccgagga
     3001 ggattggaaa caaggctacg tgcgttgtct cggagtaagg cttgctggtg accgcatcga
     3061 ggaaaaagac catctgggga atcccatttt cgacgatacg tttcttttat tgatcaatgc
     3121 acatcacgaa cctattcaat tcacattgcc cccccacccg attaacctgc aatggcagct
     3181 cgtcctcgac acggtccagg aggtcatacc cgctcacgcg ccgttgattt ctgaaggcaa
     3241 atattacgat ctcaaaggcc gctccctagc tctgttgcaa atagagacag cggtgcccat
     3301 tcgtccaccg ttgaggggat ctcccagtgc cggaagctga taagcccggc gtagggtgag
     3361 gagtatcgga ggcgagatga cccgatccgt gcgcgcttcg gtctttgcca ttacgtgggg
     3421 aacggcggtt gccgtggtgc cgaccaatgc gctcccgcag ccggcggcgg agaaaccaag
     3481 cgatgttcag ctgcaaacgg ccattgtgcg tgcaatgcaa accagcccac tcacagccgg
     3541 gagtctcatc gtcgtgcacg taaaccaagg tgaagtgaca ctcgaaggag ccgccgccac
     3601 tcctttggca cggcgggagg cgacacggct ggcggaaacg gtccttggtg tgaagggaat
     3661 ccgcaatcgc ttgagcgtcg tgaatacgca aactgctgaa gttccagccg agcagaaata
     3721 gcacacattg tctgatccct attgtgttca ttccttgggc atgttcccag gaagcccagg
     3781 gaatcgacca gcgtcacggg aaatcggctc acgctaaagt actgaaggag ggtaatgaaa
     3841 cgcccacgct ggcaaacaag gaggaaccaa tgaacgcaga tattttccaa ggcaaatggg
     3901 gacaattcaa aggtgagttg aaaaggcaat ggggcaagtt tacggatgat gacttaactc
     3961 agatcgaagg taactacgac aagtttttag gcaaagtcca agaacgttac ggcgcccaga
     4021 aggacgatat ttcaggttgg gcggataagt ggtttcaggg caacaaaccc gaacaggttg
     4081 aacgcgagcc tgtccggtaa ctcgtctgaa gaggaggtca ccatgttaag agtcgtaatt
     4141 tgcagcatca tggtcggagt gcttgcggcc ggaccggcct gggcactttt cgaatccaat
     4201 aaggagctcg tggcgggtgc caagattccc ttggaggaag ccgtcaagag cgccatcaaa
     4261 gctgtcccgg gcaaggccgc ggaggccgac ctgggcaagg atgacggacg aacaatttgg
     4321 aagatcgaag ttattgatca caacaacaag agccaaacgg tctacgtcga tgcacagacc
     4381 ggcatggcca agatgaacaa gtaacgaatt acgacataga agcggaccgg gccaccccgt
     4441 tcggtccgct tttttgtggc tacatgaatg accgcggaca taccgaatga aggtgaaata
     4501 gccatgcggc cacttcacag acctgtccgc tccgcatgcc tgttcggatt tgtcgtgtgg
     4561 gccatgatga tcgcagtccc tgccgcaggc gctgaaggac tattcgaaca agtgaacatc
     4621 ggaccggttt ccatcggagg cagggcgacc tattacgatc cgaaggacgc aagcggtgag
     4681 tggtatggcg gcgcacaggt ccggctgtat gcgtttcgct ttctagcctt cgagggttct
     4741 gccgattaca gacgaaacga tttcggcaat accagagttc atagctatcc cgtacaagtc
     4801 tccgcgttga tctatccgtt cggcacgatg agactggcgc cttttattct cggtggaggc
     4861 gggtggtact attcgacggt caaggggccg gggaactttg acgatacgca aaaccggttc
     4921 ggctttcacg ccgggggcgg tctacaattc tttttgaaca agcattggtc cattgacagc
     4981 acctatcggt acatctggct ggaaagaatc gagtccaaag atcagaacgt cgtcgacaag
     5041 aagttcaatg acaacggtca catgatcacg atcggactga acttccactt ctgaagcgac
     5101 gaagcgtatc ccgtgagaga tcagatacgg gatacaatat ccatcgagct agagcatggc
     5161 atgggcgcaa caagcggggt ggcatcgttt ctttgtgcgg tggtcttgtg cgctctttgt
     5221 ggaaccgtcc ccgcgtgggc ctcagacgag ttcatcgcgg gttatgcgtc ggcaattttg
     5281 caacatgaat tcaacgtcac ggacgctgca gtcgaggtgc acgacggctc cgtgttggtg
     5341 acaacgaaaa ctctgaccaa catcgatcga gggaaagtcg tcgcagccat caaacaggtg
     5401 ccgggagtca cacaggtcga aatccgcccc gccgatccgt ccaccatttc gccggaagat
     5461 caagccgctc atgcgtctat tccagagccg ggagccaaat ggctgccgcg cggcacacta
     5521 gtcgctccac tccatgccga cccgcgttgg ccccagtttg gagcaagcta caggcaattt
     5581 acgcaaggtt tgaaccttgc cggtgtcttc gccgcgaatt ttggtgagac gtttgcgatt
     5641 taccgcaaca aagccttcat ggacggagag tgggaattcg gggtacaggc cggtgtcttt
     5701 agcatctttg acgtcagtgc ccagtcaatc gatctcgtca atgcagatta ccgtgtagga
     5761 ttcttgagca gctaccggaa tggaccattt tccggattca tccggcttca gcatcaaagt
     5821 tctcatcttg gagacgagtt cttgttgaac aacccgggta tcacgcgagt gaatctcagt
     5881 tacgaagagt tggatgtgaa actatcctat caggtgttcc aatggcttcg ggtctatggc
     5941 ggcggaggat tcatcgtcca tcgctttccg gaagatctag ggcgaggtac gagtcaatgg
     6001 ggggcagagt tggtcagtac gaggacgttt ctgggtgggc gccttcgtcc ggtcgcttat
     6061 gccgattttc agtgtaacga gcgaaccagg tggcaggtcg gccattccat catggcaggt
     6121 gtgcagttcg agaacgcccg cattggtgac cgccagatcc agctcctcgg ggaatattat
     6181 tccggacctt caccagacgg gcagttctat ttgcaacgag cggcctggta tgggatcggc
     6241 attcatcttt acttttaaac tggttgcctc cgcctagcat cggcaatttc acttctaaat
     6301 cactatgtta cttatattgg attcatatcc aacaatcgat gtctggggtg gactgcctgg
     6361 tacttctggg agtggtccta gttagggccc accggagacc cagtgtaccg tgaagtatgg
     6421 aaattctcat gtgtgcatcg tgctggcgtg tccagcacct gtcagaagag tttggtgctg
     6481 aactgcagga atggagtgat ccaacgacat ttatggcgtc cggccaggtc agcgcgagta
     6541 aatatgacat tgtggatgga tactgcgatc cttgcctcat cgaaatagcc ctccgcgatc
     6601 aactggctct tgccagggct gaaggagaac gactcaatgc ctgagaactc cggcatgcat
     6661 tggctgacag ggaagcgggt cctgttggcc gtattatttg ccctggcatt tccgtggcct
     6721 ctcccctctc aagcccagga tacgctgtgg aatacctgga ggcagggagg attacaggcc
     6781 ttgcatgagg gacggctagc cgacgccgag cgtctgctga ctgcggcact cgaacaggct
     6841 gaaaaatacg gcctggacga tttgcgtgtg gctgatgcag cgaatgactt ggcggtcgtc
     6901 tatgctaatt caggaaagcc gctcgaagcc gagctgctct tcggcagagc cttgaccatc
     6961 ggagaaaaag gtttgggagc agatcatccc ggagtgggag caaccattca aaacctcggt
     7021 attttatatg ccactcagga acggtaccgt gaggccgagc cgctcttgaa gcggtccctg
     7081 gccatcaatc tcaaggaatt cgggaccaac catgcgcgaa cagccttgtc tctgaagacc
     7141 ctttcgagct tctatgccat tcaaggacag ctcggagaag ccgaacggct gatacacaag
     7201 tcccttgcga tcttggaaga aagtcgggtt cgacagggtg acccgcagtt gatttcgaca
     7261 ttggaagtgc tggcagccat tcttcgtaat acgaatcggg agcgcgaggc tcaagaaatc
     7321 gaagggcagc tcaaaactgc tcaagatacc acggtgtatc ggtagtccaa tgcgtacctg
     7381 ccgttcgtgc tagagggacg aacgcatcca taagggacgc ttcacggtgt ctgacgaaag
     7441 gagttcacta tgctcagctg ggctgtcaca tttctcgtcg tcgcgattat tgcaggaatc
     7501 cttggcctgt cgggtgtggc cggaacagcc acccagattg cctatgtgct tttcgtcgtg
     7561 ttcttgattt ttgccgcggt gagtttcgtc atgggacgtc gtccgccggt cacatgaccg
     7621 acaattcctg cgtggtcccg ttccttttcc ttgagtagca atgctcgaca agggcattgc
     7681 gtagggaggc ctatgaagtt gttacccagc ctagtcctgg gcgtgtggac gctgttgtcg
     7741 cactccgcgg cggaaagtgc catgcaggaa gccaagcact ttgtcgtcga catcatttcc
     7801 atcaacggtg aagagttcgt cgtcaaggat gagaacggca aggaagcaaa tatccatgtc
     7861 ggcactgaaa ccgagaaatt cggccaattg caggtcgggg accgggtcga tgcctgggtt
     7921 tacccgaatg gccaggccag gaccctgatg attcttcgca gtgcctcggt gatcaagcaa
     7981 gaccagtctc agcagaatca acaggaggct cagcagaaac agcaaagggc cgaagcccct
     8041 caggaggtgc acccgggaga agccgcaata aaatgactca gcgcctgtgg tctgcttgat
     8101 gggaaatcat ggcagagcaa aggcgggctt ttagggattg ggcggagagt ttaaaagttg
     8161 aggtagcgga caatgcagct ctgagtacca tgacctcgaa cgtgaggtca tgcacacttt
     8221 cgcgtcatcg tgacgatcgt atgatggata tcttctgcag gcgcctcctt cgaaaggaat
     8281 gcatctgccc ccgcccgtcg catggcagcc accatctcct gtgagttgtt cacagaaatg
     8341 ccgatcacgg ccgtgtccgc aaaggccgct ttgatgcgcc tggttgcctc cattccatcc
     8401 attcgtggca tattcacatc catgatcacg caatcgggcc gtagcttgtc cgcaagcaag
     8461 acggcttgtt cgccgtcacc cgcttctcca acgacaagca gatcttgatg gtcatacagg
     8521 agtaccctca actcttcacg cacgatggta tggtcatcga ccagaagcac cctgattcgg
     8581 ccgtctttaa aggcggatgt ccgctcgtcg tgttgcaggg tcccgtcctc ctggctttga
     8641 caaggcgtct ggagcttacc cgcgtccatg ctctcgggtc gcgtcagcgg tagtgtaagc
     8701 cttgacgtca tcccccggcc cggagcggat tcgatctcca gacaaccgcc gatggccaac
     8761 atgcgctccc ggatgctgaa gaggccaaac ttcgacgaca tggctgaggg ctggctgctc
     8821 tgtgcggcct gaagatccat gccagcccct tcgtcccgga ccgtgatgca caggcttgct
     8881 cccgtttgct cgatcgtcat gagggcgtga ttggtgcggg catgcttacg gacgttgatc
     8941 agcaactcgc gcactgattg gaataacagc acagctcggt cttcgggtag ggacacctga
     9001 tccgggaccg attgacgcac ctcgacggta agttcctgcc ggatcatttg ttcggccaac
     9061 catcggagcg ccacagggag accgaactca tgaagaacag gcggactcaa ttgagtgacc
     9121 agcgtccggg tatacaccag cgcctcgttg acaactcctt cggtctgagc gatttccgcc
     9181 tgagttggtc ccggtgcctt tcgcttaatc tgactgagct tcatgcaaac gagagccaga
     9241 agctgggtca ggtagtcgtg aagatccgtc gccagccgct tgcgctcccg ctgttcggtg
     9301 agattcaact ccgtcgcgag ggcgcgaagc cgttcatgca gttgcgtgag ttcttgtgtt
     9361 cgctccgcca cccgggcttc cagttcatgg gtgaatgaac gcagccgttc ttccgtccgc
     9421 ctgcgttccg tcacatcgaa gttcacgccc accatccgca cgggacgacc ctcgtggtcg
     9481 ttcaacacct ggccctttgc cgcaagccat cggattgcac cgtctggcct gatcacacga
     9541 aactcatgct ccaacgctcc ggtcgatccg cttaacgctc gttggaccga ctgctcgacc
     9601 agcggcaagt catccgggtg gacatgccga tagaattcgg gagtcgaagg tgtgatgctg
     9661 ccctccagga gaccaaacaa ggcaaactcc ttccggtccc atcgcgtcac gttggtaccg
     9721 agatcaacat cccacgcacc catttggccg gccgccaggg cgagtcgcaa acgctcctca
     9781 ctttcgcgca gcgcctcctc catccgccgt cggtcggtaa tatccatcat gacggccgtg
     9841 aaaccgatgg ctcggccctg gtgatcaaga tgtggcacat aggcgcaccg catataccgt
     9901 gttccgatac gctcatacgg cacggccgct tcgaattccg tacgcgtccc gcgcaaggcg
     9961 gcatcgatgt gggcacgaaa tgtatcgtag gcttgagagc cgatgacttc ggcgatcggc
    10021 ttgtcaatga cttggcttgg ctccagtcga aaccggtcaa cataggcgcg attgacgaag
    10081 cgcaaccgct gttgttcatc acaatacgcc atcgcgatcg gcgaactgtc ggtgaccaaa
    10141 cggaggagtt cttcattgcg acgcaaggcc tcctcagcaa ggcgccgctg cgtgatatca
    10201 gtggcgacgc cgaggatatg agtcgcttgt ccacgttcgc cgaggatggg tcgtttgaca
    10261 gtctggagcc atcgcacgct cccggaggcg tccgtgatca cctcctcggg gatgaacagc
    10321 tctttaagac cgtcgatgac ttccctgtcc ttgcgtcgga actggtcgac ttcttcgaca
    10381 ttcgaattga aatccgcatc ggttttcccc aagagctctt cgactgtcgt gccgtagcaa
    10441 tcggcaacgg cttgattcac cagcgtaaaa cgaccgtcac gatctttgac aaaaataaaa
    10501 ttgggatccg tatcgatgat ctggcggatg aaggcatgcg atcgctggag agcttcctca
    10561 gtccgtttgc gttccgtcat gtcctgcatc acgacattga ctccgagcac cgatccctca
    10621 cggttgacaa gcgggtaata gttggcaagc caatagcgga tattgccagg atctgagggc
    10681 ggttcccctt caaactcgac gttcatcgct gcctttgctt cgtcgacgac cagccgaaga
    10741 accggctcga gccgatccgc catggcagga gaaagcatct cccgaagggt gcggccgata
    10801 taggcctcag ggggaaagcc gttgatctgc gccaatgtct ggttgatgcg gacaaagcgc
    10861 agttcggtgt cgaggaacgc caggccggca gggctcgtct ggtagagtcg attcagttct
    10921 tcggcctggt attcggcttc ggtacggctt tgctgcaagt cctgttgagc ttgtcgatgg
    10981 tcgtgtatgt cggtagaggt gccgatccag cggacgatgg ctccgctttc cccgacaatg
    11041 ggaacggcgc gggctaactg ccaacgataa tggccgtctg cgcgcaagag gcgattttcg
    11101 aactcataag tcttccctat tctcaggcat tcgagccatc gctctgaaac cagttgccgg
    11161 tcgtcggggt gaatcgctcc cagccacccg aagccgatcg cttcatcaag cctgaggccg
    11221 atgtactcaa gccccggacg attcacgtat tcgacgaatc cagaggccgt cgcgataaag
    11281 acgacttgcg gcatcgtatc ggctagcgcg cgataccgcg tctcgctttc gcgcaaagct
    11341 gtttccgccg ccacacgccg gaagtgttcg gcgatgcggc cttgttcttc cgcctgccga
    11401 ctctgttccc ctaagcgttc ctgctccgcc agccgctcct tgagcgcggc attttctcgc
    11461 agcaattcgt cgtatgatgt gtagttagcc atcgaaagaa ccatgccaat gtgggatttt
    11521 caatgcatgc gtggactggt tttcatgcat ccattgaaca ttcgacgctg gtctttcgga
    11581 tcaccatccc gatcgtttcg tagaggtgat cggccgcact ttctttggtc agaaacgcgg
    11641 aggcgccggc gtccctcatt gatttcacga cgtgctccgc ctcgttcacc gataaaccga
    11701 ccacatacgt cccgggtaat tcttctcgaa tccgtttcgt cgcttcgatg ccgtccaacc
    11761 ggggcatgtt gacgtccatc acgatcaagt caggcttgag caatctcgcc aacgtgatgg
    11821 cttcttctcc gtcccctgcc tcggcaataa tgtgcagatt ggcgtaccca tcgagaatgc
    11881 tacgcaaacc ctgccgcacc atcgcgtggt cgtcgacaag gagtactcgt actggggctt
    11941 ggcgctcgtg cgccggatga tgcacactga atcctgacgc gccggacggt tgcgctgcgt
    12001 tgagcggcaa gtgatccgcg gtggcgtctt ccgctccgct cgcgccaacc gtcacgccaa
    12061 gagtgaccgt cgtaccggct cccggctgtg aatccaactc catccatccg tcgatcgctt
    12121 ccatccgctc gcgaatgctg aacagcccga acttgtcccc cttggattcg cgtatggtga
    12181 ccggatcgca gcccaccccc tcatcatgca ccacgatgtg caattttccc tggccgtcat
    12241 cgcccatcgt caccgacgcg cgatcggtcc ccgcatgttt taagacgttg aacaataatt
    12301 cgcgaacaca ttgatacaac atggtcgtat ggtcttcgga caacggagga gccacgtccg
    12361 gcacgtccac actgacgtcc aaaccctgct ggcccatgcg ttcgcccagc cagcgcaagc
    12421 cggcggccaa gccgaactgg cgcagggtcg gtggcatcag ttcggccacg accgtgcgtg
    12481 tgtaggtcag gctttgatcg atcaattggt ctgcttcctg aaggacggaa caagcggcat
    12541 gcggcgagat catttgagtc gcctgattca tcttcagtcg tgtgaccacc aacatctgcg
    12601 cgagatagtc atgcagttcg ccggcgatgc gctgacgctc tcgctgctcc gtcaacgtca
    12661 aatcctgagc gagaccgcgg agccgatcat gcgtccgcac gagctctttc gtgcgttcat
    12721 tgacccgtcg ctccaactct ctcgtccatt cctgccgctg gatgtcggac tgtttgcgtt
    12781 cggtgatgtc gaatgtcact ccgacgaagc gggtcggccg cccgccgccg ttgtaatgaa
    12841 tacgcccgta cggagcgagc cacctgagct cacccgtgtc tgcacgacga attcgatgct
    12901 ccgcctggaa gagctggccg ctcttgctgg cgttcttcat cgcctgccgt acgatcgcgc
    12961 ggtcttcttc atgcaccagg gcccaccagc gtttggccga cgcttgaccg gattcgaaat
    13021 cgtacccaag cagcagggca ttctgccgat tccacacaac ccggccagtc cgcaagtcca
    13081 catcccaggt gcccatcccc gccccttcgg tcgcgaatcg aaggcgctcc tcctgttcag
    13141 caacctgctc ggcccgttgc cgtgtttcat ggagaagacg acgtcgctcc ttcgccattg
    13201 ccacgtaatt ggaaacggcc tcgagtaact ccaggtcatc gggttcgaac gagtcgcggg
    13261 tgttgctggc aaatgccagc gtccccagta accggtcacc gacgatcagg gggaaacagg
    13321 cgtaggcggt ggagccaacc gcttgcatgc gagacgcttt aggatcagac gacgattgca
    13381 ggtgatcaac gatgaccggc tgccgtgaaa ctgccgaggt cccacagagc gcttgtccga
    13441 actgcaaaaa catgagcggt tcgagggccg gatcaggaat gcccgcccac gtcgccatgc
    13501 ggatcccgct gttgtcgggc atgacgtcat agtcgagaaa cgtgtccagg cccacgcttt
    13561 tgcgcaacga ttcgaagata tcctgcatga cgtcgcgcgg atcatccacc cgcagcaatt
    13621 gatccattgt cttactcagc aactgctggc gagcggtatg agtgcgcagc tccagttcag
    13681 cccgttttcg ggcatcgatg tcttcaatga ctcctattaa cgattggatt cggccaccgg
    13741 cgtcccactg gatcgacaag gtgaccgtca cccaaatggt ctccccgtca ggccgcatca
    13801 gtcgctgctc aagggacact tcggatatac cacccagaag gagcgtcccc acttggctct
    13861 catgttccgc gcggtcctcc ggatgcgtga ggtcgcgcag ctcctgcttc atgagatcgt
    13921 gttccgcgcg accggtgatg ctgcacagct tgtcattcac ccgcaggagg cgaccgtcgg
    13981 aaccgaccac gcacatcccg acggccgcct gctcaaataa cccccggaaa agctcctcac
    14041 tgcggcgcaa gcgggcaagg agtccttcgt tttcctgctg gcgctctcgc aagcgcagca
    14101 gcgatcgcac ggctgcaaga agttccttct cgtcgatcgg ctcgatcaag tacccatccg
    14161 agccgacctc caatccgaag gcccggtcca tcccgctcac gcgggatgcc gagacatgca
    14221 acaccataat atcggcgagt tccggccgat tcttaattcg gcgacacagg tcgaaaccac
    14281 tgatatcggg caggttgacg tccatgagca cgagcggagg ccgcacctct tctacgacac
    14341 gccaggcatc ggctccgcac gtggcgtcga tcacccgata accggcacgc tccagcatgc
    14401 gccgcctcgc atatcgcccg gcttcctgat cgtccacatt gacgatggtc gtcatctctt
    14461 cggtcataca tgcccctctg tctgatctgt cgttgtttac tgtcgtatca gttattcact
    14521 ggcctggtgg aataccattg tgcggtcgac tcgtggccaa ccggacctct gctagaccat
    14581 gccgcatagg caacccacgg aacgacatgg ggaaacttga tgggattagg cgagcacact
    14641 atgacctgcc accagggtct gcatactagg gagaccccta gtccaacctt ttagggggct
    14701 tcgtcaagat cggtcatccg aaagctgaag aaaaacagga tcgtgtactg gtcatgatag
    14761 gtgcggtggc tgtgccaggg ggtggtatag accaagcgac cggacgcccg ttctacgacc
    14821 tccactccat acttaatgta tccgtcctga gacaaattct ggtagacggt cagttggggc
    14881 aaggagaagg gaatcaggac gctttgaatg ggaggcatgc cgaaaaatga cgtcgcttga
    14941 atcgtgccaa acgattgaac gacaacccgc accacataaa cagcctcgtc ttcgcgtctc
    15001 gacatgcggt agcccagaac cccaaggtgg ctggcgacag cgtcgctgat aaacgccaga
    15061 tcacctgcag gagaaaaata gccctctgtc ggaggggtgg cggttgaagt agaagcggac
    15121 cccatggaac cggtggcgag gggcaggagc ggtgacatat agcccaactg aagaccgctc
    15181 actttcatat aaagcggttc ggcaggcgaa accggtagtc tcaggtcgag caaactgcgg
    15241 ttgagcgcct gtgtcagcag caattgctca atggcgcttc gaggtgtttt ggtgatttcg
    15301 cggggcaagg tacaggatgc gagcagaggc acaatcgacg cccagatgat ggcgagcgtc
    15361 cgataatgca cgattagcac cgctatttgg cgggcggcgg aggttcgtgg acttccgccg
    15421 gcagcgtttc gatgactttg agaacccggc cttcctcatc aagctgcatc gtcacgcgat
    15481 cgccgatcga aaaccgttcg agcaaatcag gccgatcgag tctgaaaaag atcggctttt
    15541 ccagatccgt cagcagcatg ccttttccag cctcaatgtc cagcttcgcg agctttcccg
    15601 tgacccggag ttgagcatcc ggttgctcga cagggacggt ttcctctgat cggcccgtga
    15661 gcggactcgt aaccaagacg aggaggatca cgtagagact tcccctcaag atggtcaatc
    15721 cgtatgcaat gatattgcgc atagaggaac cttttaccga gattggctca atcattttca
    15781 ctacaccgta tgtctagcgg gtcctctact aggcgatccc ccagttctga gcagttgaga
    15841 aatggctcac cgtgaggcat gcatctccgt ttgacgcact atgtcctcgt catgggcctc
    15901 ctttttagtg gtcaggcttg ctcaaaagct tcactcgtac acccgccgct gaagatacac
    15961 atgcccgacg cagatacaac cgcgttcggt cggaaccagg aggcccgagc ctcggcgagg
    16021 cagctctcca gagcgagtat gctcgatcca ttacttcgag tttcttcgcg cctcatcacc
    16081 gctgcccggc actctgagta cgccacagcc cgaccagtat ttgagtggga gatcgaagtc
    16141 tgcgaagacg agactgtggt acaacccttt gtgcacccag agggctcgat catcgtgtgt
    16201 tcaggcacct tccgagttgc agagactgag gcaggccttg cagcgcttct cagtcatgaa
    16261 ctggcccatg cgttgaagca ctgcgcttct ccagctccgg aactctgcca taggccggac
    16321 aggcacaatg agacgctgtt tacaatcgat caggagatcg aagcggacgg aatcgggatg
    16381 aacttaatgg ccgatgcagg gtatgatccg tccgaattat tactgatttg gaagcgtatg
    16441 aaagagagtc atcgagaacg gggagatgcg ctcctcgtgc acgtgaccta cgatcgccga
    16501 atggatcaca tcaaaggcaa gctgccagac gcacttcgac tctacgcaga aacgcaccgt
    16561 gcgcctcagc ggcctttacc cgttcgataa ccaaccacgg tatcgaacag acgtcagact
    16621 tctgaaagga gtgaagagaa tccagacaga tgacccgcga ccgttctcag gcagcaggca
    16681 acgtaaccgt atacgtgccg gcccgattcg agaaaagacg cagctcgccg gttcgactta
    16741 agcgatcgac ttccaaaaag acctgattcc acgtgtaggt ggagcacgct tctagcaggt
    16801 cttccatatc acaggcctgt ctgtgcttga ccatctggac aattttgcca gcgatctcgg
    16861 atggcagacc ggcatattcc ggggtgaagg tagaagtggg caccagttgg ttcatcttct
    16921 cctcctttgt tggaagggac acattagtga aggaccgtac gccgagttct ttgatgaacc
    16981 tactaggtag taccctagtt tttatctgga acaattttcc ctccggatga cccatttttt
    17041 ctccaccgct gggacgttac caccactggg acagtggagt tgatcttggg ttctacccag
    17101 ttggcgcccc cattggctgc ctcatagagt gtgggaacac gtgtgacgct cccgttccgg
    17161 atgcgaccaa tgaacagcgc tcaaccccag gccaagtcca atgagctgga ccttgaggat
    17221 cagccgagag tgcacgtcgg gtgcggactt tgcggatcca gacgtagccg tctggtgtgc
    17281 tccccggaag atattgtcgt gcagcagagg tttctcgaac atttctaccg ctctcgctgg
    17341 tcgcaacaca acgctgccac tgcaaccgac cgcgtgagct tcacccagga ttatgccacc
    17401 tccatcgtcg cgtgcatgga ttgcggcctc ctctaccgaa accctcgtcc ccgcgcagaa
    17461 gcagtcactc aggcttatga aactgaagac tacgacgagg cgtatttgca ggccgagttt
    17521 acagcacagc aaacttgggc gcgtggcaaa gcagacatcc ttgctcggca tcttgaaaaa
    17581 attggtcgca cgggtagacc ccgcgtgttg gaaatcggca gctttgtcgg tggcttcctg
    17641 cttgaaggac agttacgagg gtgggacatg ctcggtgtgg atcccggtca tgacgtcgcc
    17701 tcattttgcc gtcggcatcg gctacccata ttcgaaggaa cgctcgagga tgcgcaactt
    17761 cctccggcct cattcgacgc aattgcgatc tggaacacgt tcgaccagct tccggaccct
    17821 cgcaaactgc ttcaccagtt gctaccgttg atgcgcaacg gcggcttgct cgtcgcgcgg
    17881 gtccctaacg gtgcgtgttt cgagtggggg atgtctatgc gggctcgcct gccgaaagtc
    17941 ttccgccgac cgctggacat tgcactggcc tgtaacaatc tcctgacatt tccctacctg
    18001 tatgggtact ctgcgaagca gctcgaagaa ctgacagagc ccttcggctt ccggctactg
    18061 gcctgctatg ccgatcagat cgtttcaagc ccaccgggtc atctgcgtcc gtgggcctta
    18121 tgggaggagc gggtggtaaa aaccctgtgc aaagcgtttg cagttctctg gccggacaat
    18181 cgctcgcttc gctttcgttc cgcgccgtgg ttggattgca tttttgaacg ggcttgtacg
    18241 gatgaagagc gtccggcacc ccagaagtta ggcctagggg ttatcccagt atactcaccc
    18301 ctagtcttca aggacactgg cctagattgc tcggttaatc ggtaggatcg gaaaggaggc
    18361 ttcacatgaa ggcgacggtt ccgaaggctt ctttcacatc gttactggtt tcaaaactct
    18421 tcagttccgg gaccctacgt cgtgccggcg caaggcagca accggcacgg tcgggtcgcg
    18481 gcgtagattc atggctggtt ttcaatctga cccggatgag cagccccgga ctcgggcgtg
    18541 acaaatgaag gacgacgagc ggtcttcacc cgtctgggag gcacgactgt ccgggtttgc
    18601 gtcgacatgg cgatccgcat atcggtaatt gccccgcaat tgatgcaacg gtaaccgctg
    18661 cagtgaaaag ggccgtcgaa gtagtcaaat accttgaaac ctccgcatcg ctcacaactc
    18721 atgctcgcat ctccttggat agaggttggt tgtcaggcgg cgtcggtttc cgcccttcct
    18781 gtttcagtga gagaagcaca aaggctgcca cgcatgaaat ttccccctac tcgtttgaaa
    18841 atacgggaag aacttctatt gtgccagtgg aggagactgc agaagcagag cgagccacgg
    18901 gacaacttgt ccccagcgag acaagaacat gattgacgag tacccgggac aagctcccga
    18961 taaacgcgcc agactcctgg tcacgattgt gctgcttttc accgtgacag tggcgacgtt
    19021 cgccgccgat cggctcttgc cgctcggagt ggcggtgtgg atgctctatc tagttcccgt
    19081 tgtgctgacg acccaattgc tgccgcaata cacgggtctg gcattggcca tcagcagcgg
    19141 actcctgctt ttcggagtgc tcgattcgaa ggaggggatt tcctacgagt tggcggtgat
    19201 caaccgaggc ttgggcctga tcgtgttgct gttcacgggg ctgtttctcc ttcaacaccg
    19261 tcggatggcg gaagccttac gggagagcga gggtcgctac cgcttgcttc tcgagcagca
    19321 cggacgcgct cgagctgaag cggcgctgca cgaggttcaa gaacgcttca ccagcatctt
    19381 ccgatcgtca cacgatgcca tcgcctatgc gacgttcgac ggcagactgc tggaggtcaa
    19441 cgacgcgttt gtgcgcgtat ccggtcgtcc acgagaggcc ttgttgagcc ccgagccacc
    19501 cacctcgctc tggccgtctc ttggattgtc gccggaacaa ttccaaaagc tccgggaaac
    19561 cggcaagcca ttggtctacg agacaatgtc ccgtcaggat ggaccgggag ctgaatggtt
    19621 gaatgtccat gcattcgccg tcagggggtc cgatggcgcc atttctggct tagcggtcgt
    19681 gattcgcaat atcagcgacc ggaaacaggc tgaggctgaa ctgcgaagta cgagcgaaga
    19741 actgcgcgca aagaatgaag cgctcgcccg caccaatgaa ctcctgtctg ccgcccaaca
    19801 cgccgcggcg agggcacagc gcctttcggc catcggacaa tttgccgcga cggtggctca
    19861 taagatcggc acgccgctga ccgcgttgtc cggacacgta cagctgctca tggaggattt
    19921 atccctcccg tcaaaagtcc gggggcgatt gcagaccgtt gaagcgcaaa ttgagcgcac
    19981 atctcggatc atccaggatc tgctgctcta tacgagacgt gctccaccgg tgcggacgaa
    20041 aatcgacatc aacgaatgcc tgcgggagtg tgcgagtctc tttcggactg aatgcgaacg
    20101 gcagcacgtg gcatgcatta cggaactgac ccccgacctt ccgccggttg aggcagatcg
    20161 tcaacacatg ctggaagctt tgaaccacct cattgaaaat gcacttcagg ccatgcctaa
    20221 cgggggatcg ctgtgtctgc ggacggcgag catcgaaccg gtcaccgtcg gccgcggctt
    20281 gcatcacaca ggcggagtgg ccattgaaat cgccgatacc ggtcacggca tcaaaccgga
    20341 ccactcggca caaatctttc aaccgttctt cacgacgaag caagctggtc gtggaacggg
    20401 tcttggtctc gctattgtcc aggaaacagt gcgagcgcat gatggccgta tcaccgtcga
    20461 gagcgaaccg ggcaaaggta cgacgttcac tatccatcta ccggccgttg ataaaggaac
    20521 accctgatgg acgaccctgc caacattcta gtcgtcgatg acgatgagga aacggtgacc
    20581 ttgcttcggg agatcctctc gaaagaaggc tacaatgtcg acaccgccgc cgacggaaaa
    20641 attgccctgg ataagattgg cagccggccg cctgacctcg tcatttcgga cattcatatg
    20701 cccgaccttg acgggatgag ccttctggcg gaactccgcg tccggtttcc cgacgtgccc
    20761 gtcattttga tgacggctta cggatctttg aaaaccgccg tcgacggcct ccgttcggga
    20821 gcgttcgact atctgagcaa accatttatc gtcgacgaca ttcgcctggt ggttcgccgg
    20881 gccctcgagc acaaaaacct ttcccgccaa aaccaggcgc tgaaggaaca gctcagacac
    20941 cgctaccgct tcgacaatct ggtcggcagc agtccgggca tcgtgtcggt ctataaatca
    21001 atcgcacgcg tcgccaatac ggaaagcaca gttctgctcc agggggaaag cgggacggga
    21061 aaggaattaa tcgcccgcgc gatccatgcc aacagtgcgc gaagcgcagg ccctttcgtt
    21121 gcggtcgacg gcggctcact tgctgaaacg ctcctggaat ccgagctatt cggtcacgag
    21181 cgaggttcgt ttaccggagc cgtcacgtca aaaaaaggct tgcttgagaa ggcgcatttg
    21241 ggcacgtgtt ttctggacga agttgcggat ctctcgcccg ttctgcaggg caagctgttg
    21301 cgcgtgatcc aggaacggga gatccggcgc gtcgggagca cgacgccaat gacgatcgac
    21361 gtgcgcatgg tcgccgcttc aaaaaaggat ttggagcagc tggtaaaggc cggaaccttt
    21421 cgcgaagatc tgtattatcg cttgaatgtc gtcacgatca acattcctcc tctgcgtgaa
    21481 cgggcggaag atatccccct gctgacgcag tacttcattc agatgtacgg cgggaaaaaa
    21541 tctcctcccg tcacgggcat ttccccggag gccatggccc tcctgacccg atattggtgg
    21601 ccgggcaaca tccgggaatt ggaacatgcc attgagcgcg ccctggccct tacgcctcat
    21661 ccggtcatct ttcctgaaga cttgccgcaa tcgattcaaa gtgcgacggt acaagccgct
    21721 gcgcaagccc gcgggtgggt gacgctggct gagttggaaa aggaccacat ccttcgagtg
    21781 ctggaggccc ataaccagga tttaggccgg gccgcaacga ttctcggcat ccatcgcaag
    21841 actctcctca gaaagcttcg ctatttcggc ttcccttgtt agacggacac ctcgtccgcg
    21901 cctggacatg gcgtccggca cgtcagcact tctcttcctg aaggcgcaag aatatttgcg
    21961 atttcgcttc cggcatgtcc cgatgtcggt ccggaccttg ctaccaacaa tcgcatcatc
    22021 aatacgcctg caagggggtt ctgcaatgat ggatgggtac ggcaagcggg tgctcgttgt
    22081 tgaggacgac gatgatgttc ggacgctcat tggcgtgatt ctgagggaga caggttacaa
    22141 cgtctacgaa gcatgcgacg gcttggaagc catcgacgcc ctcaagaa
//
LOCUS       DASSCQ010000024        31128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1701321, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000024 DASSCQ010000000
VERSION     DASSCQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 31128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701321"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFH05_01660"
     CDS             <1..211
                     /locus_tag="VFH05_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676952.1"
                     /translation="FREVSTANALSDHRRIIVKQAEALRTFCSGCERLLFYIRTNGAL
                     AEDEARMVEHYCKEILAKVGQSNSS"
     gene            complement(503..1729)
                     /locus_tag="VFH05_01665"
     CDS             complement(503..1729)
                     /locus_tag="VFH05_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676953.1"
                     /translation="MRNQWVMSLSLIASIGFGILSTASATAATACDAVLGKWAWFAGG
                     EVTIKGDGTFTQKSGNSGTWECLDSGKGAVALKWKQGAYLNRLILADGGTKLVSTDPS
                     QSFVKATRINPESALADLLAPPQDTKSDPSSSRRPTGQPGGTVPIKPSTPLTGSQKKG
                     PISYESPQGFVSSDPKVEARFKQGYDLFMKKNYAAAFPILMETAQAGHPRAQALLGRT
                     YQEGLGQAKDYKQAAEWYGKAAAQGHRTAQYSLGNLYFEGDGVAKDQVKAIHLYRQSA
                     DQGYDQAEFILGLAYEFGWGGLPRDRRTAIKWLDRAGRHKNGGAGFIATWLEKPDTPQ
                     FKNEVQLGKYIGATIDRRASAAFGSGNSGGGGENPCSIYSGPVAAACNQGDSGAMDRY
                     REHQENQEDKRKYGVQ"
     gene            complement(2017..2092)
                     /locus_tag="VFH05_01670"
     tRNA            complement(2017..2092)
                     /locus_tag="VFH05_01670"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2057..2059),aa:Lys,seq:ctt)
     gene            complement(2183..2259)
                     /locus_tag="VFH05_01675"
     tRNA            complement(2183..2259)
                     /locus_tag="VFH05_01675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2223..2225),aa:Arg,seq:ccg)
     gene            complement(2326..2745)
                     /locus_tag="VFH05_01680"
     CDS             complement(2326..2745)
                     /locus_tag="VFH05_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6676954.1"
                     /translation="MHSILVVDDNEDIRNALQLVLRKAGYRPLLAESGQHAIDLMKEE
                     SLTSSVAAVLCDLEMPGMDGSAVIAHFRAHYPLIPIIVLSGATPNQFLDAVGQQGVGD
                     WIRKPATNEAVLEKVRGAVHLYEVRQADRRAKNFPTP"
     gene            complement(2820..3419)
                     /locus_tag="VFH05_01685"
     CDS             complement(2820..3419)
                     /locus_tag="VFH05_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET6676955.1"
                     /translation="MPAHGRSHLARLARKTDEWFHRASAALLGQVACRIGCSHCCIGL
                     FPVTRLDAELLHEGLAQLPSDKRLLIQQRARAQVSALEAAYPNLLASRSLDGWSDEEI
                     DHAVSRFDQDRCPALSDDGLCSLYAYRPLTCRSMGIPTQVGQMVEGACCVQTFVPIVR
                     VSESLRAEEQELASLESAALTTHGPSAQGEEILLPYAFL"
     gene            complement(3425..4180)
                     /locus_tag="VFH05_01690"
     CDS             complement(3425..4180)
                     /locus_tag="VFH05_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676956.1"
                     /translation="MMDVSSQKPAGVYPSVLGLSTEKHAPGVGKRLHGGLAVSRPSIV
                     EYWHAAAEIWCQTASVIEDLFMGKQLPILPATAQPAETAAEKFIYSRVFCQKENPSPL
                     RLMIDFLKSRGQLPILPPDMNDEALDEWAWVHVALQYSRARKPIQVFCFRDRGTYQEA
                     FEQEKGQFLEALTSYDDVEASLVADTLKKARFILLTRHDPADITEEGYDFNGWILEFY
                     QENCDGIVQIDGQGFYSPKGTLIVDMSAPSPEE"
     gene            4277..4564
                     /locus_tag="VFH05_01695"
     CDS             4277..4564
                     /locus_tag="VFH05_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6676957.1"
                     /translation="MAKSMTKSQIADHVAQKAGLTKKAAVQLLDDFAALAYREAKNVF
                     VVPGIGKLVLANRKARMGRNPQTGEAIKIPAKRVVKFRVSKAAKDAILGKK"
     gene            complement(4700..4957)
                     /locus_tag="VFH05_01700"
     CDS             complement(4700..4957)
                     /locus_tag="VFH05_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6676958.1"
                     /translation="MGQLQTADGERDVACELASPMTVRQVIQRQGVQLRHLLQLIREK
                     KVLVTINKKIASDDSLVHDGDAIRLVGHDGFGGSGLGPTHS"
     gene            5042..5464
                     /locus_tag="VFH05_01705"
     CDS             5042..5464
                     /locus_tag="VFH05_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676959.1"
                     /translation="MPQLDVHNPGMPDLQFVLFVSALCTSNLESINVPQDLRARIFDR
                     CWALINTDAPPVDPKQRVLDLRQGTELTLEACVSTIRSLLAEAGITKLMWDHPVSNPT
                     RDSSAAAQPLIDRLGQLYPADPDIVDPPPSRSDGRSTG"
     gene            5481..6044
                     /gene="rfbC"
                     /locus_tag="VFH05_01710"
     CDS             5481..6044
                     /gene="rfbC"
                     /locus_tag="VFH05_01710"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET6676960.1"
                     /translation="MKVTPLDIPGALLIDPSLFRDRRGLFCETFHAQRYVEAGLNDQF
                     VQDNFSRSVRGTLRGLHYQQPHAQGKLVMAVHGTIYDVVVDIRKGSPTYGTWQGFELS
                     SDNFRQLYIPPGCAHGFCVTSEEAAVLYKCTDFYSPGDERGVIWNDPALRISWPVSTP
                     LLSPKDQAYPSLAATEHELPVYSPSTR"
     gene            6135..6560
                     /locus_tag="VFH05_01715"
     CDS             6135..6560
                     /locus_tag="VFH05_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HET6676961.1"
                     /translation="MSKSLKGTKSHENLKGAFAGESQANRRYLYFARRADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFQELAEWFETLAKAERSHANRFQKGLDSLKG"
     gene            6643..7911
                     /locus_tag="VFH05_01720"
     CDS             6643..7911
                     /locus_tag="VFH05_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6676962.1"
                     /translation="MKGLTLLSSVDTAQLQKETLRIYEICDGCRRCFNLCPSFSTLLN
                     GIDRNEGDVTKLTPDDHRRIVDECYYCKLCFNHCPYTPPHQYEIDFPHLMIAGKRQLA
                     LERGVSWRDRWLNRTDLLGTIGSWTAPLSNWLIRSPIVRLLADVIGGVHRDRQVLHFS
                     RETFPRWFAKRTRPARPSAKKVALFSTCLVNYQVTDVGKATVQVLEKNGVEVVVPEQR
                     CCGMPGFDTGDVNAIQRTIDANVASLHSWVAQGYDVVVPAASCSLMLKREYPELRPNE
                     QTKAVARSTFDICEYMMKMKKDGQLSMDFVKKPGRVAYQIPCHLRDQNIGFKSKDLME
                     CAGAQVELIEKCSGHDGAWSAKSEFFGLSMKIAEKAVRTIQQQPADVIASDCPLAGLQ
                     LDQAGASAHVGGKAALHPIQIVRDAYGLGR"
     gene            7908..8492
                     /locus_tag="VFH05_01725"
     CDS             7908..8492
                     /locus_tag="VFH05_01725"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HET6676963.1"
                     /translation="MKPLTREDLLSLQDYERQRDSFRSRIIALKQRRRLALGPLISVV
                     FENRETLQFQVQEMIRAEHIQDPLKVQDELNVYNGLLPAEHELSATLLIEITDEATMK
                     EWLDRFMGLDRGHTVGINAAGETVYGQFEQGHSHETKISAVHFVRFRPGREMTRAFAD
                     LRQPVRLMVDHNGYRETVAVPGSLREEWLADLAS"
     gene            8519..9271
                     /locus_tag="VFH05_01730"
     CDS             8519..9271
                     /locus_tag="VFH05_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET6676964.1"
                     /translation="MPPVLLTKRIEFAAAHRYYKPEWDEAKNRAVFGRCFNPPAHGHN
                     YMLEVTVSGEVDPNTGMVINLFDLKRVLLAVLEEFDHKNLNLDMPYFASQIPTSENLA
                     RVLWTKLVTQQDIGVLHAIKLHEDEDLYAEVTAAAGVDVASVTRRYSFVAGNGTQEGR
                     EWDCFVTLNGPVDPVTGMVTDLGLLDRLVQDTIVKKFDHQDLRTFFGGRLVSGETLVK
                     EIWHALETSTKSGVLSRVRLVESRDLFYECSG"
     gene            9292..9771
                     /locus_tag="VFH05_01735"
     CDS             9292..9771
                     /locus_tag="VFH05_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6676965.1"
                     /translation="MLRRCWVVIGGVIWVSTAWAGPDEDAVERVLLAEAKSSATFAET
                     RDRQAVLKLYTSDYNGVQDGEVETRESIEKWLSEYGSALDQGSRVHFLGTVSNVKTHM
                     AGSMAWATYDYVFQAIRNGELDAQDRGRCTSVLRKEPSGWLIQHEHCSKTKPQGMER"
     gene            complement(9850..10182)
                     /locus_tag="VFH05_01740"
     CDS             complement(9850..10182)
                     /locus_tag="VFH05_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET6676966.1"
                     /translation="MSGIVEDVNDGNYKEFTDSLGAVVAYGLATCEPCKTYDPILEDT
                     AARFPAIKVGKAKMHVPGRCREIKKTHTFETYPTTHFFANGKLLLTREGVVDQAELAA
                     LISDYLLK"
     gene            complement(10255..10725)
                     /locus_tag="VFH05_01745"
     CDS             complement(10255..10725)
                     /locus_tag="VFH05_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET6676967.1"
                     /translation="MSDVAAEIKVGDTAPDFNLKDQDQQDVKLSDYKGKKNVVLCFYP
                     LDWSPVCQGENKCLTDDFPKFQNANAELFGISCDSFFSHKAWADSLDLKHRLLSDVHR
                     TTAKAYGLYFEPLNCSKRATVIVDKNGKVAYVKVQEIKVAREDKDILSALSKLN"
     gene            11066..12655
                     /locus_tag="VFH05_01750"
     CDS             11066..12655
                     /locus_tag="VFH05_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6676968.1"
                     /translation="MLSRYLYVTRNLGCCLFLALMGTLILLGAADAAEDQREYDFDLS
                     KGIYFYNQARYGEAERHLRDAYNAKPTDPTAGYYLGLNSIKLQRYTEAEERFREVLRR
                     KPDDARARMGLGIALYHQNRYPEASAELSVAERTIKNDPLLYYYAGFAAARQQDDELA
                     SDKFLKAGKLDPELANNAHYLRGAGLYSQGDYQQATAEFRSAIEGAPAAAALPSKPAV
                     APSTQQPSKRWSANYGLSLQYDSNVVLLPGGSTLPGGISHKDDFVTALNLGGEYRFLQ
                     TDTWTIGAGAGLFTNFHARLSDFNVLDLAPTLYVQRQLGSAQLRFQYTLDYVTVGGDA
                     YLLSNALQSTLTIPESQRTYTQAFFRYQNKDFKTFRDDELGVPVNQTRDANNFMLGAL
                     QYWRFAEDRGYLRGGYTFDTDVTGGGDTPTTLGLPSKADWSYTGHRLSLGAAYQPLTA
                     TTLLLGMDYYRQNYNNPNSFSSDGAIRKDNVYQLTGTVVRDLQSWLWLAFQYSLTRDQ
                     SNVEVFDYTRHVISLTLGGAF"
     gene            12664..13608
                     /locus_tag="VFH05_01755"
     CDS             12664..13608
                     /locus_tag="VFH05_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET6676969.1"
                     /translation="MNIRAFVLSVSVAVSLGCGIAAAADVPDTAGIVLAVQGTVTMQP
                     ATMTAPVKLRPFDEIGPLAILDSQAGSRCKVLYADDSLVTVGDNSRLEVTEQTYQSGS
                     DQRAFVAHLIRGSVRALVARSFEGDNSTFEIDAGTSAVTARGTYFVTWLNGPSPRKGK
                     GEEEEGLEMEGATGVANIGRNGNVAFTSGGATVLVLPGQFAIAMPGAPPSMPASIQTH
                     IVPVATAISGTMLPDMPKQESPREALAEAGIIGGGTKGQTLAGPVMAKGGSLGGQAPS
                     GSYMLPDWPFPVTPVTPPAVASGAAFSNTTVNLTIRLP"
     gene            13651..15393
                     /locus_tag="VFH05_01760"
     CDS             13651..15393
                     /locus_tag="VFH05_01760"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676970.1"
                     /translation="MHTRHRTIFHPALGLVLVALLAGCGSENNGQTVAVNLSLIVNSR
                     QAQQGPASRVFAWLERWFPGATPAWAQSASEIARIEVQVSGPGIAVPVTASVPVSNPT
                     SGQEIPVSMQVPVGPNRTFTVAAYNVANEKIFGGTLPNVNLTAGAPINLPPIELKRLF
                     TVTVQKHPEGTGDGTVTSTPVGIDCGGTCLGQFEEGTTVSLNAAAAPGSVFAGWSGGC
                     SGTGPCSVTTTATVTARFNVAVATDRLTVILAGTGPGSVTSNPSGIACPGICAGDFAT
                     GTTVTLTAAPTNGSTFDGFSGAGCSGPGSSCTVVMVGSPTVTATFTAAPPSFTLTVTK
                     SGAGLGIVDSDPPGITACSTTCSATFVTGTIVNLTATPQGGSTFVGWGGACSGAGACS
                     VDMSANRAVTATFDPPVSLSTLTLQKQGTGSGNVTSNPSGINCGGTCPVQSAPFGTGS
                     SVTLTATPDFGSVFTGWAGCSGGSTCTTLMSGDQTIVAIFDAAPPDLVTLTVTKQGSG
                     DGTVTSQPAGIVCGAACQFSYQRGTVVTLTAAPDANSSFNDWHGGGACDNSSSPTCQL
                     VMSDNQTVSAHFDD"
     gene            15417..16925
                     /locus_tag="VFH05_01765"
     CDS             15417..16925
                     /locus_tag="VFH05_01765"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676971.1"
                     /translation="MRPQHKGTGLPSAWGIVLIAVVAACGSDSPTDQTVAVNLSLVVD
                     SRQVQHRPAPSRLFAWLDRWFPGATPAWAQSVSEIASIQVQITGPGIPSPATSTVPVS
                     DPTSGQEIPVSIQAPVGPNRTITVTAFNAQSPPGKIFGGTLVGVNLTAGAPVNLAITL
                     VRLFTVTVQKEGEGSGAVTSTPGGIDCGVTCVNQFPEGATVVLNAAAAPGSAFAGWSG
                     GCSGTGACSVTGNATVTARFTVPVSTDRLTVTLGGNGTGTVTSNPSGISCPGTCAADF
                     ATGITVSLTAAPTNGSTFNGFSGAGCSGSSSSCTIAINGDQAVTATFTAVIPIPTSTL
                     SVSRIGSGVGTVTSTNQAGINCGSVCSASFPTGSVVSLTATPSTGSTFIGWSNGCTGT
                     GSCDVTLNADQTLVAQFDLVPDVVTLSVNTSGPGDGTVTSDQGGISCPNACSASFTRG
                     TTVVTLTATPVGNSVFAGWRGNPCEAFGTGPCMITMDDNRSANARFERGGGR"
     gene            complement(16935..17687)
                     /locus_tag="VFH05_01770"
     CDS             complement(16935..17687)
                     /locus_tag="VFH05_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676972.1"
                     /translation="MRIKKKSPKPSAKRPPLYFIGYRGPAPQKEELIFWYNGQYGGPL
                     TIDHEGGAPESWHIRHGPWTAHVVIPLPSTHVVGLKDQLAWEHDQIGAIAPSVMPPRD
                     MPDTILFAARLARGLVLLTQGTAHDLTTQQYLNPSDWQDRSLIEFVADEHVLVAQDDQ
                     AQSDMFWCYSLGLSKFGLDELETFLPRGTPDRTAKELLGESIREIIRIGQSPKVGASF
                     TLPSLNRTITIANHRTAAPAGRMLGFREVQAS"
     gene            complement(17767..19617)
                     /gene="typA"
                     /locus_tag="VFH05_01775"
     CDS             complement(17767..19617)
                     /gene="typA"
                     /locus_tag="VFH05_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET6676973.1"
                     /translation="MSLRVPNERRNDIRNIAIIAHVDHGKTTLVDALLRQTHVHRKID
                     DMGERIMDTMDQERERGITIRAKNASVIYKGVKINIVDTPGHADFGGEVERTLRMVDG
                     VLILIDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVVNRTFDLFVHLG
                     ATDDQLDFPLVYTSAVNGIATLDINKPGTDISPLLDTVLEKIPGPAINANGPLQILVL
                     ALLYDSYKGKMGVGKIQSGSIARRQQVLVLGKDGAAPGKVSDLAVYSGLERTDVEEAK
                     AGEIVAVAGLDQVSIGDTIADAVQPSPLPRVTIDEPTVQMTFSVNNSPFAGREGKFLT
                     SRHLRERLFKELETNVSLRVQETDSPDRFLVAGRGELHLAVLIEQMRREGYELQVSQP
                     EVILHREGGKVTEPYEELTIQVPETYQGAVIEEIGKRRGEMRHMKLVHSDAGPSEMHL
                     EYHIPTRGIMGLKNILLAKTRGTVIMHHVFKAYETANEQDLLVAPHGSLCAFEDGKST
                     GYALFMTQERGVMFIGPGVEVYRGMVVGENSRDEDLDVNVCKEKHLTNMRASGSDEAL
                     VLTPPQEMSLEFALEYIGQDELVEVTPNNLRIRKRLLHPEDRRKAKKSGK"
     gene            19773..22157
                     /locus_tag="VFH05_01780"
     CDS             19773..22157
                     /locus_tag="VFH05_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET6676974.1"
                     /translation="MTLIDRPVQLSDTTRQPPPRRPWRRWHITLITLAALFLAALLAT
                     VGLLWYYAQDLPELSQLQNYQPSLVTQVYSSDQQLIGQFFIERRILTPLAQIPEHLRR
                     AVIAVEDVRFFEHPGLDYIGILRAAWTNLRRGGKVEGASTITQQLARSLFLSSERTFD
                     RKVRELVLAYKMELVSTKEQILETYLNQIYFGQGAYGVASAAQSYFGKDLAGLTVAEA
                     AFLAGLPKSPNHFSPFKNYARAKKRQEHVLERMEEAGFLTSAQREEALAEPLSFRRPG
                     SEQTAPYFVEYVRQHLVAKYGEAMVYKGGLRIKTTLNLGMQRAAEAAFKTGLRELDKR
                     QGWRGPLRTVDPATLTTGTQPVATTDQPVAIGDEREAVVTKSGKDVFWVQIGNVSAKL
                     LFDDMAWAKRRLTGPDLAKDIAVASTPKQVLKPGDVIEVKIKKIDKEQVYVQLEQTPL
                     VEGGLIALDPDKGAIRAMVGGYDFNRSEYNRAVQAHRQPGSAFKPIIYATAINQGMSP
                     ASVVLDAPVVYEQVEEEKTWKPENYGKRFHGMVSLRDALAHSHNLATVRLLDKVGIKN
                     VIEFARQVGITSPLSADLSLGLGSSSVGLLELTSAYGVFLNQGNRAEPFAIISVTDNA
                     GEVLEQEDVQTQEVLSKETAYLITNMMEDVVQKGTGQAAKSLGRPIAGKTGTTNEFIN
                     AWFIGGTPNLVAGIFVGFDDRRSLGETESGAHAALPIWITFMKEALRTLPVVPFTIPE
                     GITFVKVDPSTGLLNTEQEDQPGNVEIFARGSEPTQAVQRRSDPMDFYKIDQPE"
     gene            complement(22173..22385)
                     /gene="iscX"
                     /locus_tag="VFH05_01785"
     CDS             complement(22173..22385)
                     /gene="iscX"
                     /locus_tag="VFH05_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HET6676975.1"
                     /translation="MDLKWQDTEDIAIRLVEEHPGTDPLTVRFTDLHAWIVALPDFKD
                     DPTKSNEKILETIQMAWHEEYQDSAS"
     gene            complement(22437..24266)
                     /gene="dnaK"
                     /locus_tag="VFH05_01790"
     CDS             complement(22437..24266)
                     /gene="dnaK"
                     /locus_tag="VFH05_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6676976.1"
                     /translation="MARIVGIDLGTTNSLVAYMKDGTPTVIAGRSGRTMVPSVVALTD
                     NGLIVGDSAKEHLTRNPARTVYSVKRFMGKGLADVQSELSYFPYSLTEQGGVIRIQLG
                     EKSYSPPQISAMILKELKQRAESHLGESITKAVITVPAYFNDSQRQATKDAGMIAGLE
                     VLRIINEPTAASLAYGLQEKTQGTIAVYDFGGGTFDISILKLKNGIFEVLATNGDTHL
                     GGDDVDRRVADLFLTEIRKAFEIDLSAYPDHMQIVRLEAERAKIRLSDELKTNIAIDL
                     PDGKGRFTRELTRDQLETVTADIIERTLSPCRLALQDAGLSPADIDEVVLVGGSTRMP
                     LVRQRVESLFGKKPHCHLNPDEVVALGAAVQADILTGGTTDMLLLDVTPLSLGIETMG
                     GVMSSLIRRNTTIPASAKELFTTYVDGQTGVDIHILQGERELVKDNRSLARFRLKVPP
                     LPAGVPRIEVTFLIDANGILNVKASDMRTGQTQSIEVKPSYGLSDTEVERMIEDSFKF
                     AQQDVDARKLIEARLEASALMTTTEKSLADGDKLIGREEAAAIRDRLSLLKAASDGND
                     PKAIRARMAELEQAAKPLSVAMLDDSLKRSLKGKKVSEVSKRD"
     gene            complement(24271..24990)
                     /gene="hscB"
                     /locus_tag="VFH05_01795"
     CDS             complement(24271..24990)
                     /gene="hscB"
                     /locus_tag="VFH05_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HET6676977.1"
                     /translation="MDHQSGSYNSGRRDLQMARSMCWHCQSEVAGEYFCERCVKVQPV
                     SKEADYFTCLGFPRRLTIDLGTLEAKFYELSRAFHPDFYQNKSETEQAISLSNAATLN
                     TAYRTLRDPIQRAEYLLDLEAGSVKEIRSSPPADLFEEILELQDTLEEFRTSDQSTET
                     GKQLRTQLQTAQAALERRKREMEEQLHQLFRQWDDLQEKGEATSGARADRERLLKQMR
                     EILSNRTYVKNIASDLVATIG"
     gene            complement(25068..25424)
                     /locus_tag="VFH05_01800"
     CDS             complement(25068..25424)
                     /locus_tag="VFH05_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET6676978.1"
                     /translation="MDSTKTETQVPVITLTDAAIKEVKRLINVQGITEGGLRLGVKGG
                     GCSGLSYTVNFDEKIGQYDQVFDFEGVKVIVDTKSAIYLQGTQLDYQKDLMGGNFKFV
                     NPNANKTCGCGESFSA"
     gene            complement(25442..25825)
                     /gene="iscU"
                     /locus_tag="VFH05_01805"
     CDS             complement(25442..25825)
                     /gene="iscU"
                     /locus_tag="VFH05_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HET6676979.1"
                     /translation="MAYSDKVVDHFNNPRNMGSFKKEEDGVGTGMVGAPECGDVMKLQ
                     IKVQNDTIVDAKFKTFGCGSAIASSSLATEWLKGKTLEEAQKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKSETK"
     gene            complement(25878..27095)
                     /locus_tag="VFH05_01810"
     CDS             complement(25878..27095)
                     /locus_tag="VFH05_01810"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HET6676980.1"
                     /translation="MKFPIFLDNHSTTPMDPRVLEAMLPYFVEKFGNAASRNHAFGWA
                     AEEAVEAARKQIARLIKADPKEIVFTSGATESDNLALKGVLEMYKEKGNHIITSATEH
                     RAVLDTAKSLEAKGLATVTYLPVDKQGMVNPEDVRKALTEKTVLISVMLANNEIGTIN
                     PIQDIGKIAKERGVLFHCDATQGVGKIPVDVQAMGIDLMSFSAHKIYGPKGVGALYVR
                     KKNPRVRIAAQIDGGGHERGMRSGTLPVPLIVGFGKACELCEQEMPAESARLANMRDR
                     LQAGIMAALEESYLNGHPTNRLPGNLNISFAYVEGESLLMGMKEIALSSGSACTSATL
                     EPSYVLRALGVGTELAHSSIRFGLGRFNTDQEIDYTIKKVIEIVTKLREMSPLYEMAK
                     EGVDLKSVQWAAH"
     gene            complement(27092..27565)
                     /locus_tag="VFH05_01815"
     CDS             complement(27092..27565)
                     /locus_tag="VFH05_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET6676981.1"
                     /translation="MLKISKKTDYALMALQHIASVQFGDVTPGRVVNTKEIAEEYNIP
                     LELLAKVLQSLSKSGMIESHNGPKGGYHLARNARAITIAQILESIEGPLGITDCSHEK
                     EGEFCMQREHCNIRTPLLKVQDSIYQLLNNMTLQDMLGGTPLITIQSPTSQGAER"
     gene            27685..28101
                     /locus_tag="VFH05_01820"
     CDS             27685..28101
                     /locus_tag="VFH05_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6676982.1"
                     /translation="MGGTNPYIEKADVELPQKPYTVTYVAPSGTVTKVQVDPARIPYG
                     QTGLPGSLLDIAMANGVDLEHVCGGVCACSTCHVIVKQGLETCNEGTDDEFDQLDEAP
                     MTTLQSRLGCQCVPNGTKDVVVEIPAVNKNLVKEGH"
     gene            complement(28135..28839)
                     /locus_tag="VFH05_01825"
     CDS             complement(28135..28839)
                     /locus_tag="VFH05_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6676983.1"
                     /translation="MAEFIQPATVLSSTNLTPHVRQLVVLPKEQSIVFKAGQWVSLKL
                     PVGIKPPLNRAYSMAAPGTTSGELILVLDRVPGGLGSNYLFLAKPGDELILSGPYGNF
                     LLPPQIDRELLFVTRYTGLVPIRCMLKHLYAERYRGPVLLIAIAPEEQELLFHQELLT
                     LAVTHPGFRYLPLVAAGGEQQAVDLTLPMLRPLLQASPQVLPMLCGTKAFVKPLRAHF
                     LEAGYDRKEVKTETFD"
     gene            28965..30386
                     /gene="gltX"
                     /locus_tag="VFH05_01830"
     CDS             28965..30386
                     /gene="gltX"
                     /locus_tag="VFH05_01830"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET6676984.1"
                     /translation="MTQVRVRFAPSPTGFLHIGGVRTALFNWLYARQEHGVFILRIED
                     TDQSRSTDESIQAIVEGLKWVGLDWDEGPYRQTERMTLYREHAMALFERGQAYWCICK
                     AEELEARRKEAEGKGLSPRYDGRCRTLGITHPSGDAALRFKAPQEGQTVVDDLIKGKV
                     VFENNVLDDLIILRSNGYPTYNFSVVVDDALMHITHVIRGDDHLTNTPRQIPIFEALG
                     FPVPRFGHLPMILGADKTRLSKRHGATSIMAYKTMGYLPDAMVNYLVRLGWSYGDQEI
                     FTRQELIERFSWKQVQTSAAVFNPDKLLWMNAEYIKTSPPSEVAQALVPYLEQTDLKD
                     QLQEVPKEWLAQLVLLVRERTKTLVEMVDWVKPYFGEAVTLEEEAAKKYLTQAVAPVL
                     KKLTDRIGSLPEFSKPALEGCFKTLVEEEGLKMGQLAQPVRVALTGRTASPGLFEVME
                     ILGPERTRLRLRQGIERAEARQA"
     gene            30423..30496
                     /locus_tag="VFH05_01835"
     tRNA            30423..30496
                     /locus_tag="VFH05_01835"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30455..30457,aa:Gln,seq:ctg)
     gene            30536..30609
                     /locus_tag="VFH05_01840"
     tRNA            30536..30609
                     /locus_tag="VFH05_01840"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30568..30570,aa:Gln,seq:ttg)
     gene            complement(30625..>31128)
                     /locus_tag="VFH05_01845"
     CDS             complement(30625..>31128)
                     /locus_tag="VFH05_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6676985.1"
                     /translation="QAPNFAKEWQDYNIAEVLEARLKKPVRVANDADVQGFGAISGTG
                     VELVVTLGTGVGTSLFVDGRLVPNLEIGRDKLRDAALQKVGKKRWNKRLVKFIKRLDE
                     IFHFTKMYIGGGNSRAVDVGMLPANVTIISNLNGLIGGIALWRDERVSSIEVATQQDI
                     TGRPLAA"
BASE COUNT         6564 a         8644 c         8929 g         6991 t
ORIGIN      
        1 ttttcgagaa gtctcaacag caaatgcgtt aagcgatcac cggaggataa ttgtgaagca
       61 ggccgaagca cttcgcacct tctgtagtgg gtgcgagcgg ttgctgttct acataaggac
      121 gaatggagct ctcgccgagg acgaagcgcg gatggtcgaa cactactgca aggaaatcct
      181 ggccaaggta ggccagtcaa attcgagttg aacgagaact tcaaaaccag acgtgttaga
      241 cgactcaagg tcgcattgag gtgagtccgt ccgtctggga ctcgaaccga tttgaacgtt
      301 ctagtcgatt gcaagttgtc gtaccaaggg aacgctgacc cgcgatttgg ttattcgcgg
      361 gtaagcggag acctgcttaa cctgatattt ccctatgatt atgcatcggg ggggaaacac
      421 aggggaccgg tgacatccac agaaggttaa acagccaggc gcgagtcccg tcgaaaacct
      481 atctcgggac actcaggagc attcactgga caccatactt ccgcttatcc tcctggtttt
      541 cttgatgctc tctgtaacgg tccattgctc cgctatcccc ctgattgcag gcagccgcca
      601 caggccctga atagatgctg caggggtttt caccgccccc accgctattg ccactgccga
      661 aagccgcaga agcccttcga tcgatggtgg cgccaatata ctttcccaat tgaacctcat
      721 tcttgaattg cggcgtgtcg ggcttctcta accaggtcgc aataaaacca gctccgccgt
      781 tcttgtggcg cccagctcga tccagccact taattgcggt gcgacgatct ctgggaagtc
      841 caccccatcc gaattcataa gccaaaccca aaatgaactc cgcctggtcg tagccttgat
      901 ctgcgctctg ccggtagagg tggatagctt tgacttgatc ctttgccacg ccgtcgcctt
      961 cgaaatataa attgcccagc gaatattgcg cggtgcgatg gccctgcgcc gccgccttgc
     1021 cgtaccactc tgcggcctgt ttgtaatcct ttgcctggcc caagccttcc tgataggtcc
     1081 ggcccaacag cgcttgggct cgggggtggc cggcctgcgc agtttccatc aggatcggaa
     1141 aagctgcggc atagttcttc ttcatgaaaa gatcgtagcc ttgcttgaac cgcgcctcga
     1201 cctttggatc ggaactgacg aacccttggg gagattcata tgaaatgggc cctttttttt
     1261 gtgaacctgt aagaggtgtg gacggtttga tcggcactgt cccgccaggt tgtccagtgg
     1321 gcctgcgaga tgacgatggg tctgatttgg tgtcctgagg aggggccaac aagtcggcga
     1381 gcgcactttc cgggttgatc ctggtcgctt tgacgaagga ttgcgagggg tccgtgctga
     1441 ccagtttcgt ccctccatct gcaaggataa gtcggttgag gtatgctccc tgcttccatt
     1501 tgagcgcaac cgcgcccttt cctgaatcta aacattccca cgttcccgaa ttaccggact
     1561 tctgcgtgaa cgttccgtca ccttttatcg tcacttcccc cccggcaaac cacgcccact
     1621 tgccaaggac ggcgtcgcag gcggtggcgg cggtggccga ggctgttgac aaaatgccga
     1681 aaccgattga agcgatgagc gagagggaca ttacccattg attgcgcacg ccattctcct
     1741 ttcagagctt tacaggcgaa tgggtatcag gctatgacac ataccttcgg ccattgcgtt
     1801 cctgcatctt gttcctccct atcacatttt gaattgacct agattttcac taggatagga
     1861 ttttttggag atgcgtgtct atcgttcagg tacagctggc tctacggagt ggcggtaata
     1921 atgatgtcca acaccggcta aaaagggctg gccacacggt cctagacggt tccgtgatag
     1981 cgagagatgt aagggtggtt tctagtccgt ggaaagtggt gggccgtgta ggggtcgaac
     2041 ctacggcccg ctgattaaga gtcagctgct ctaccaactg agctaacggc ccaccggggg
     2101 attcaatttt actgccttta ctggagggat tctccgcggg cacccaacaa ccagtaagcc
     2161 cgcggcacga gccagctcgg tttggtgcgc ctgacaggat ttgaacctgt ggccctcagc
     2221 tccggaggct gatgctctat ccagctgagc tacaggcgct cctgctacaa ggaaatcacc
     2281 atagcacagc attttacgct gtgtccaccg gctcgatcta gagggctaag gagtgggaaa
     2341 gtttttcgct ctcctgtcgg cctgacgtac ctcgtaaaga tgcactgccc cccgtacctt
     2401 ttcgaggacc gcttcattcg tcgccggttt tcggatccag tcacccacac cttgctgtcc
     2461 gactgcatcc aggaactggt tcggggtggc tccgctcagg acgatgatgg ggatcagcgg
     2521 ataatgcgca cgaaagtgcg cgatgacggc cgacccgtcc atgccgggca tttccaggtc
     2581 gcatagaacc gcggcgacgc ttgaggtaag gctttcctcc ttcatgagat cgatggcgtg
     2641 ttgcccgctc tcggccaaca aggggcggta accggctttc ctcagcacca gctgtaacgc
     2701 atttcgaatg tcctcattat catcgacaac gagaatgctg tgcacgaatc ccactccggt
     2761 tgacgccgag ggtatcgcgt tgatgcgcaa aatgctagta gcccagtagc acgcgggtat
     2821 cagaggaacg cataagggag gaggatttcc tcgccttgtg cggatggtcc atgcgtggtc
     2881 agcgctgctg actccaagct cgccagctcc tgctcttctg cgcggagcga ctctgagaca
     2941 cgaacgattg ggacgaatgt ttgcacgcag caagcgccct caaccatctg tcctacctga
     3001 gttggaattc ccatcgaacg acaagtaagc ggacggtacg cataaagcga gcaaaggccg
     3061 tcatcgctta gggcggggca acgatcctga tcaaagcggc tgacggcgtg atcaatttct
     3121 tcatcggacc agccatccag agagcgcgaa gccaacaagt ttggataggc agcctcgaga
     3181 gcggacacct gagcacgtgc cctttgttga atgagcaggc gtttgtcaga tggcaattgg
     3241 gcgagtcctt cgtgaaggag ctcggcgtcc aggcgtgtca caggaaagag gccgatacaa
     3301 caatgagaac agcctatacg acaggccacc tggcccagca aggctgcgct ggcccgatga
     3361 aaccactcat cggtttttct cgcaagtctg gcgaggtggg aacgaccgtg ggccggcatg
     3421 ggccctattc ttctggcgat ggggcggaca tatcaacgat caacgtgccc tttggagagt
     3481 aaaaaccttg tccatcgatc tggacgattc cgtcgcagtt ttcctgataa aactcgagga
     3541 tccacccgtt gaagtcgtag ccttcttccg tgatgtcggc cgggtcgtgc cgggttagga
     3601 ggatgaaacg tgctttttta agcgtgtccg caaccaacga ggcttccaca tcgtcatagg
     3661 aggtgagggc ctccagaaac tggccttttt cctgctcgaa cgcctcctga taggtgcccc
     3721 ggtcccggaa gcaaaacacc tgtatgggtt ttctagcccg agaatattgc agtgccacgt
     3781 ggacccatgc ccattcgtcc agtgcttcat cgttcatatc gggaggcaga atggggagct
     3841 gaccacggga tttcaggaaa tcgatcatca ggcggagtgg agagggattc tccttttggc
     3901 agaacacccg ggaatagata aatttttcgg cagcggtttc agcgggttgt gccgttgccg
     3961 gcagaatagg aagttgctta cccatgaata aatcctcaat aaccgatgcc gtctgacacc
     4021 agatttcggc cgcggcgtgc cagtactcta cgatgcttgg tcttgaaact gcaaggcctc
     4081 catggaggcg tttccctacg cctggagcgt gtttttcggt tgacaggcca agaacgcttg
     4141 gatagactcc cgcaggtttt tggctggaaa catccatcac ggtctctttg acgagtcact
     4201 tcatcgcacg aagtctccac tgagaggcag cagcccgcaa ttcacctcat tctgttttta
     4261 agggagggtt tctcgcatgg ctaaatcaat gacgaagtcg cagatagccg atcatgtggc
     4321 tcaaaaggcc gggctgacca agaaagcggc cgtccaattg ctggatgact ttgcggcctt
     4381 ggcatatcgg gaagcgaaga atgtctttgt ggtgcccggt atcggtaagc tcgtgctcgc
     4441 caatcgcaag gcacgaatgg gacgcaatcc ccagacaggt gaagcgatca aaattcctgc
     4501 taagcgggtg gtcaagttcc gcgtgtcgaa ggccgccaaa gacgccattc tcggtaagaa
     4561 gtaaccaatc atcgggcgcg aagctcacac gctttccgcc ttgcgcctgt ctggacgaag
     4621 ggccggctcc ctctagggcg ccggcccttc ccattcgctg aaacacaaga tcgttttgtg
     4681 aactgattcg ctcaccgagt caggagtggg tcggccccag accgctcccg ccgaacccgt
     4741 catggccgac gagtcggatc gcatcgccgt catgcaccaa cgagtcgtcg ctggcgattt
     4801 ttttgttgat cgtgaccagc accttttttt cgcgaatgag ttggagcaag tgccgtagct
     4861 ggacaccctg gcgttggatc acttgccgca ccgtcattgg cgaagccaat tcacaggcga
     4921 cgtctcgctc gccgtcggcc gtttggagtt gtcccataag ggagatagtc accatatgat
     4981 ttacccactc tccactagac ccggttgact ccatctgaac gccacttgat aatgcccggc
     5041 catgccgcag ttggatgtcc ataatccggg gatgcccgat ctgcagttcg tgctctttgt
     5101 gtcggcgctc tgtacctcga atcttgaatc catcaatgtg ccgcaagatc tccgcgcgag
     5161 aattttcgac cgatgctggg cattgatcaa caccgatgcc ccgcctgtcg atccgaaaca
     5221 acgggttctc gacctccggc aggggaccga actcacgctc gaggcctgtg tctccacgat
     5281 tcgatcactc ttggctgagg ccggcattac gaaactgatg tgggatcatc cggtgagtaa
     5341 tccgacgagg gacagctcag cagcagctca accgttgatc gatcgactcg gtcaattgta
     5401 ccccgccgat ccggatatcg tcgatccccc gccatcccgg tcggatggtc gttcgacggg
     5461 atgactccag gactgacgcc gtgaaggtca ctccactcga tattcccggt gcactactga
     5521 tcgaccccag tctgtttcgc gatcgccggg gactattctg cgagacattt catgcgcaac
     5581 gatacgtgga agccggtctc aacgaccaat tcgttcaaga taacttttct cgctcagtgc
     5641 gggggacgtt acgaggcctt cactatcaac agccgcatgc ccaaggaaaa ttggtgatgg
     5701 cggtccacgg tacgatctat gatgtcgtcg tcgatattcg gaaggggtct cccacgtacg
     5761 gaacctggca ggggttcgag ctgtcctcgg acaactttcg gcagttgtac atacctcccg
     5821 gctgtgcgca tggtttctgc gtgaccagcg aagaagcggc cgttctctac aagtgcaccg
     5881 acttctattc gccgggcgac gaacggggcg tgatctggaa cgatccggcc ctccgcatct
     5941 cgtggccggt gtccacgccg ctgctttccc ccaaagacca agcctatcct agtttggctg
     6001 cgactgagca tgagttgccg gtgtacagtc catcaacccg ttgatcctgt actaatcgct
     6061 gggctatctt agagccccta caatggtgga cgtccctgag agtggttgat gggggattta
     6121 acgaggagga ttcgatgagc aagagcttaa aaggaacgaa gagtcatgaa aacctcaagg
     6181 gcgcctttgc cggtgagtca caggcaaacc gccgctacct ctatttcgct cgacgggcgg
     6241 acatcgaagg gtatccagat gtgggcggtc tctttcgcga tacctccgag gccgaaacgg
     6301 gtcatgcctt cggccacttg gactttttga aggaagtggg cgatccggca accggagtac
     6361 cgattggcaa tacggaagcc aacctcaagt ctgccattga aggtgagacg tatgagtaca
     6421 cccaaatgta tcctggaatg gccaaaacgg cgcgcgacga aggcttccaa gagctggcag
     6481 aatggtttga gacgttggcg aaggcagagc gatcccatgc aaaccgtttt cagaaagggc
     6541 tggacagttt gaaaggctga gagcggcaac gggcgcatcg gaggggtgga ctgaatccga
     6601 tttggtccac ccttcgtgtt tgaggaagcg tttgggggaa agatgaaagg gctgactctt
     6661 ctgagttcgg tggacacggc gcagctgcag aaagagacgc ttcgcattta tgaaatctgc
     6721 gatggatgtc gtcggtgctt caacttgtgc ccttcgttca gcacgttact caacggcatc
     6781 gaccggaatg aaggggacgt gacgaagttg acgccagacg accatcgccg aatcgtcgac
     6841 gaatgctatt attgcaagct gtgctttaat cactgtccct acacgccgcc tcatcagtat
     6901 gaaatcgatt ttccgcactt gatgattgcc ggaaaaagac agcttgcgct tgaacgcggt
     6961 gtgagttggc gggaccggtg gctgaatcgg accgacctct tgggcaccat cggcagctgg
     7021 acagcccccc tcagcaattg gctcatacga agccctatcg tccggctgct ggccgatgtg
     7081 ataggtggcg ttcatcgtga ccgtcaggta ctgcattttt cccgcgaaac gtttccgcgc
     7141 tggttcgcca agcggacgag gcctgcccgc ccgtctgcca agaaagtggc cttgttttct
     7201 acctgtctcg tgaattacca ggtgacagac gtgggcaagg ctactgtgca agtgttggaa
     7261 aaaaacggcg tcgaggtggt cgttccagaa cagcgttgct gcgggatgcc cgggttcgat
     7321 accggagacg tgaatgcgat tcaacggacc atcgatgcga atgtcgcctc gctgcactcg
     7381 tgggtggccc aggggtatga tgtcgtggtg ccggccgcca gttgcagctt gatgctcaaa
     7441 cgggaatatc cggagctgcg gcccaacgag cagacgaagg cagtggcccg aagtaccttc
     7501 gatatctgtg aatacatgat gaagatgaaa aaagacggcc agctctcgat ggatttcgtg
     7561 aagaagccgg gccgtgtcgc gtatcaaatc ccatgccatc ttcgtgatca aaacatcggg
     7621 ttcaagtcca aggacctgat ggaatgtgcc ggagcgcagg tggagctcat cgaaaagtgc
     7681 tccggacatg atggggcatg gtctgccaag tccgagtttt tcggcctgtc catgaagatc
     7741 gccgagaagg ccgtacgcac tatccaacag cagccggccg acgtgatcgc gtccgattgt
     7801 ccgctcgcag gtttacagct ggatcaagcc ggcgcttcgg cccacgtggg cgggaaggcc
     7861 gcgctgcatc ccattcaaat cgtccgtgat gcctatggcc tgggtcgatg aagccgttga
     7921 ctcgagaaga tctgttatct ctgcaggact atgagcgcca gcgcgactcg tttcgttccc
     7981 gcatcatcgc cttgaaacaa cggcgacggc tggctctcgg gccgctcatc agcgtggtgt
     8041 tcgagaatcg ggagaccttg cagttccagg ttcaggaaat gattcgagcc gagcatatcc
     8101 aagaccccct gaaagtgcag gacgagttga acgtctacaa tggtctgcta ccggcggaac
     8161 acgaactcag tgccaccctc ttgattgaga ttaccgacga ggccacgatg aaggagtggc
     8221 tcgatcgatt catgggactg gaccgcggcc atacggttgg catcaacgct gcaggagaga
     8281 ctgtgtacgg gcaatttgaa cagggtcaca gtcacgagac gaaaatcagt gccgtgcatt
     8341 tcgtgcgatt ccggcccggc cgggagatga ccagagcttt tgccgatctt cgtcagcctg
     8401 tgcgcctgat ggtggaccac aatggatacc gggaaacggt ggccgttccc ggcagtcttc
     8461 gtgaggagtg gctggcagac ctggccagct aagccgccgg tcttctccat aggagtgtat
     8521 gccgcccgtt ctgctcacca agcgcattga atttgccgca gcccaccggt actacaagcc
     8581 ggaatgggat gaggccaaaa accgtgccgt gtttggacga tgtttcaatc cgccggccca
     8641 cgggcataac tacatgctcg aggtcacggt ttcaggcgag gtggatccca acacgggaat
     8701 ggtcatcaat ttatttgatc tgaagcgcgt cttactcgca gtgctcgaag aatttgacca
     8761 caagaatttg aacctcgaca tgccgtattt tgcgtcgcag attcccacgt cggaaaacct
     8821 cgcccgggtg ctgtggacga agctcgtcac gcaacaggat atcggagtgc tccacgccat
     8881 taaattgcat gaggacgagg atctgtacgc ggaggtcacg gcggccgcag gtgttgatgt
     8941 cgccagcgtg acgcgacgct attcgttcgt cgcgggcaac ggaacccagg agggtcggga
     9001 gtgggattgc tttgtcaccc tgaacggacc cgtggatcct gtgacaggga tggtcaccga
     9061 tctcggtctg ctcgaccgct tggtgcagga tacgatcgtg aaaaaattcg accatcagga
     9121 tctacgaacg ttctttggtg gcaggctcgt ttccggcgag acactcgtca aagagatttg
     9181 gcatgcgctg gagacgagca caaaatccgg tgttttgtcc cgcgttcgtc ttgtcgagtc
     9241 ccgcgacctc ttttatgagt gctcggggta gacaccgttg gggaggattt catgttacgc
     9301 cgctgttggg tcgtcatcgg gggtgtgata tgggtgtcaa ccgcatgggc gggccctgat
     9361 gaggatgctg tcgagcgcgt gctgcttgcc gaggcaaaat catctgcgac gttcgccgaa
     9421 acgagagata ggcaggctgt gctcaagctc tatacaagcg actacaacgg tgtgcaagac
     9481 ggcgaggtcg aaacccgaga gtccatcgaa aagtggttgt cggagtacgg atccgcccta
     9541 gatcaaggca gcagggtgca ctttcttggc acggtctcca acgtgaagac ccatatggcc
     9601 ggctctatgg cctgggcgac ctatgactac gtgtttcaag ccatccgcaa tggcgagctg
     9661 gatgcgcagg atcgcggcag atgtaccagc gtcctgcgca aagagccatc gggctggctc
     9721 atccaacatg aacattgctc caaaacgaag ccgcagggca tggaacggta atggatgtcg
     9781 cgagctgagt cggctcactc accggggtga aagtgcatga aataccgcgg ggcgtccgac
     9841 cgtgtgatgc tacttgagga gataatcaga aatcagcgcg gccagttctg cctgatcaac
     9901 aacgccttcc cgggtgagaa gcagcttgcc gttggcaaaa aaatgcgtcg tcggataggt
     9961 ctcaaacgtg tgggtctttt tgatttcccg acagcggccg ggcacatgca tcttcgcctt
    10021 tcccaccttg attgccggaa acctggcggc cgtgtcctcg agaatgggat cgtaggtctt
    10081 gcatggctcg caggtcgcca atccataggc cactaccgcg cctaggctgt ccgtaaactc
    10141 tttgtaattc ccgtcgttga cgtcttcgac gatgcctgac atatcagtgg taccgctcga
    10201 tgctgaggac tgcgatcgct gtcaatcggc agccctcagt cgtgaccggt gctcctagtt
    10261 cagtttcgag agtgcagaaa gaatgtcttt gtcttcccgt gccaccttga tctcctgcac
    10321 cttcacatag gcgaccttgc cgttcttgtc cacgataacc gtggcgcgct ttgagcagtt
    10381 gagcggctcg aaatacagcc cgtaggcctt ggcagtcgtc cggtggacgt ctgacagcag
    10441 gcggtgcttg aggtccaagg aatccgccca tgccttgtgg gagaaaaagc tgtcacagct
    10501 aatgccgaac aattccgcgt tcgcgttttg aaacttcggg aaatcatcgg tcaggcactt
    10561 gttctcaccc tgacagaccg gactccaatc cagcggataa aaacacaaca cgacgttctt
    10621 cttgccctta tagtcgctga gcttgacgtc ctgttgatcc tggtccttca ggttgaagtc
    10681 gggagcggta tcgcccacct taatttctgc ggctacgtca ctcatcagat gtcctccttc
    10741 gcatggttgt tctcatgaca tgaaagccgt agttttgtac cgtggtcctg aaaagcttgt
    10801 caacggggcg aaagacctac gattttcggt catctcctgc cgtaagctct agtttggagc
    10861 cctccatcac ccttgtttga aggacaggat gtccccaagt cgatgctcat gggacagatt
    10921 gtcctgagcg tgtaatctca cctgcttcaa gggcttgtaa tcgttccttc ttttgtcgtt
    10981 ccggcatagg tccaattctt gagtaagccc tcgtccgccg actacgacgg gagtgcatcc
    11041 ggggagtgca tccgaagtga atcgtatgtt gtcacgatac ctttacgtca ctcggaatct
    11101 cggatgctgc ctgtttcttg cgttgatggg aacactcatc ctgctcgggg ctgcggacgc
    11161 ggctgaagat cagcgtgagt acgatttcga tctctccaaa ggcatctact tttacaatca
    11221 agcccggtat ggagaggccg agcggcatct ccgcgatgcc tataatgcca agccaacaga
    11281 tcccacggca gggtactacc tcggactgaa ttccataaaa ctccagcggt atacagaggc
    11341 agaagagcgg ttcagggaag tgctgcggcg gaagccggac gatgctcgcg cccgcatggg
    11401 gttgggcata gcactctatc accaaaaccg ctaccccgaa gcttcggccg aactgagtgt
    11461 cgccgagcgc acgatcaaaa atgatccgct tctctattat tacgcagggt ttgccgcagc
    11521 gcgtcagcaa gacgacgaac tcgcctcaga caaattcttg aaggcaggaa agctcgatcc
    11581 ggagttggcc aataatgcgc attacctccg cggagcggga ctctattcac aaggtgacta
    11641 tcagcaggcg acggcggagt tccggtcagc gatcgagggt gcccctgccg cggcggcact
    11701 gccgtcaaag cccgcggttg ccccgtcaac ccagcagcca tcgaagcgat ggagcgccaa
    11761 ttacgggctc agcctccaat atgattcgaa tgttgtcctg cttccaggtg gttccacgct
    11821 gccaggagga atctcccaca aggacgactt tgtgacggcc cttaaccttg gcggtgagta
    11881 ccgattcctg caaaccgata catggacgat cggagccggt gcgggattat ttacaaactt
    11941 tcatgcccgc ctaagcgatt tcaacgtgct cgacttggct ccgaccctct acgtccagcg
    12001 tcaactgggc tcggcccagc tccggtttca gtacacgttg gattatgtga cggtgggcgg
    12061 cgacgcctat ctcctctcga acgcgcttca gtccacgctg actattccgg agagccagcg
    12121 cacctatacg caggcgtttt tccgttacca aaacaaggac tttaaaacgt tccgagatga
    12181 tgaattgggc gtgcccgtga atcagacgcg ggatgcgaac aacttcatgc tcggcgcgtt
    12241 gcaatattgg cgctttgccg aggaccgtgg ttatctccga ggcggctaca catttgatac
    12301 cgacgtcacg ggcggcggtg atacgccgac gactctaggc ctgccgagca aagccgattg
    12361 gtcctatacc ggtcaccggc tgtcgctggg agccgcctac caacctttga cggccacaac
    12421 gctgctgctg ggcatggatt actaccgtca gaattacaat aatccgaact ccttctcttc
    12481 agatggggcc attcgtaaag acaacgtcta tcagttgacc ggaacggtcg ttcgcgacct
    12541 gcaatcctgg ctgtggctgg cttttcaata ttcgctgaca cgggatcaat cgaacgttga
    12601 agtcttcgac tacaccagac atgtcatttc actcacgctc ggaggcgcat tttaggaggc
    12661 atcatgaaca ttcgtgcctt tgtcctgtcc gtgtctgttg ccgtgtcgct gggttgtggg
    12721 atagcggcag ctgccgacgt acccgatacc gccggcattg tgctggccgt ccaggggacc
    12781 gtgaccatgc agccggcgac gatgacggcg ccagtgaagc ttcgtccatt cgacgaaatc
    12841 ggaccgcttg ccattctaga tagccaggcc ggctcacgct gcaaggtgct ctatgccgat
    12901 gacagcctgg tcacggtcgg tgacaacagt cgtttggaag tgacggaaca gacctaccag
    12961 tcgggcagcg atcaacgggc gtttgtcgcc cacctgattc gaggatcggt ccgcgcgctc
    13021 gtcgcacgct cgttcgaagg agacaactcg acgtttgaga ttgatgcggg cacatcggcc
    13081 gtcaccgcga gagggaccta tttcgtgacc tggttaaacg ggccgtctcc acggaagggg
    13141 aagggtgagg aagaagaagg gttggaaatg gaaggggcca ccggagtggc gaacatcggg
    13201 cggaacggca atgtagcctt cacctccggc ggggccaccg tcttggttct tcccggacaa
    13261 ttcgcgattg ccatgccggg ggcgcccccc agtatgccag cctcgatcca gacgcatatc
    13321 gtgccggtcg cgacagcgat ctcagggacc atgctgcccg atatgccgaa gcaggagtcg
    13381 ccgcgtgagg cgttggcgga ggcagggatt atagggggtg gcaccaaagg gcagacactg
    13441 gcgggtcccg tcatggcgaa gggtgggtcg ctgggaggcc aggcgccatc gggaagttat
    13501 atgctcccgg attggccctt tcctgtgacg ccggtgacgc ctcccgccgt cgcctccggc
    13561 gcagcgttca gtaacacgac ggtgaattta acgataagac ttccataggt tcacaaccgg
    13621 agccattttt ggcaatttga ggtgggcgcg atgcatacca gacacagaac catttttcac
    13681 ccggcgctgg gtctcgtact cgttgcgctt ttggctggct gcggatctga gaacaatggt
    13741 cagaccgtcg ccgtcaattt gtcattgatc gtcaattcac gccaggcgca gcagggacct
    13801 gcctcccgag tgttcgcctg gctagaacgc tggttccctg gtgcgacgcc cgcgtgggcg
    13861 caatccgcat cggagatcgc cagaatcgaa gtccaggttt ccggaccagg gatagcagtt
    13921 ccggtcaccg caagtgtccc ggtgtcaaat ccaaccagcg ggcaagagat ccctgtgagc
    13981 atgcaagtgc cggtcggacc caaccggacg ttcacagtcg cggcctataa tgtggcaaac
    14041 gaaaaaatct ttggaggcac gctgcccaat gtaaatctga ctgccggagc cccaatcaat
    14101 cttcctccaa tcgagctgaa acggctgttc accgtcactg tgcaaaagca tccggagggc
    14161 accggagacg gcaccgtgac gtctacgccc gtcggcatag actgcggtgg gacgtgtttg
    14221 ggacagtttg aagaggggac gacagtgtcg ttgaacgccg ccgcggcacc cggttctgtc
    14281 tttgccggct ggagcggcgg atgttccggc accggtccct gttcggtcac cacgaccgcc
    14341 acagtcaccg ctcgcttcaa tgtggccgtt gccaccgatc ggctgacggt tatcctcgcg
    14401 ggcaccggcc ccggcagcgt caccagtaac ccgagcggaa ttgcctgtcc tggaatttgc
    14461 gcgggcgact ttgcgaccgg tacaaccgtg acactcaccg ccgcaccgac gaatgggtcg
    14521 acgttcgatg gatttagcgg cgcaggttgt agcgggccgg gctcgtcctg cacggtcgtc
    14581 atggtcggca gtccgacggt cactgcgacc tttaccgctg ctcccccttc cttcacgctt
    14641 acagtgacga agtcaggtgc aggcctaggt attgtggata gcgacccgcc tgggattacc
    14701 gcctgctcga caacctgcag cgccaccttt gtgacgggca caatagtgaa cctgaccgcc
    14761 acaccgcaag gcggttccac attcgttggt tggggtggag cctgttcagg ggccggggcc
    14821 tgttctgtgg acatgagtgc aaatcgagcg gtaaccgcga cgtttgatcc ccccgtcagc
    14881 ctgtcgactc tgacactaca aaagcaagga acaggatcgg ggaatgtcac aagcaatccg
    14941 agcggaatca attgcggagg gacatgccca gttcagtcgg ctccctttgg gacgggaagt
    15001 tcagtcacgc tcacagccac tccggatttc ggctccgtct ttaccggctg ggccggttgc
    15061 agtggaggct caacctgcac cacgttaatg agcggcgatc agaccatcgt ggcaatattc
    15121 gatgcagctc cccccgatct cgtgacactg actgtaacca aacagggaag cggtgacggg
    15181 acggtcacga gtcaacctgc cggcattgtt tgcggcgctg cgtgtcagtt ttcttatcaa
    15241 cgcgggacgg tcgtgacctt gactgccgca ccagacgcga attcatcctt caatgattgg
    15301 catggaggcg gcgcttgtga caattcctcc tcgccgacct gtcaactggt gatgagtgat
    15361 aatcagacgg tgtcagctca cttcgacgat tgattgggta gcttccgagg ggcgtcatgc
    15421 gtcctcaaca caaaggcaca ggtctccctt ctgcttgggg catcgttctt atcgcggtcg
    15481 tcgcggcatg tggttctgac agccccactg accaaaccgt cgccgtaaat ttgtccttgg
    15541 tcgtcgattc acgtcaggtc caacatcggc cggccccatc ccggctcttc gcctggctag
    15601 atcgctggtt ccccggtgcg acgcccgcgt gggcacaatc cgtctctgag attgcctcaa
    15661 tccaggtcca aatcacggga cctggcattc cttcaccggc cacaagcacg gttcccgttt
    15721 ccgatccgac gagcggtcaa gagatccctg tgagcattca agcaccggtc ggcccgaatc
    15781 gaacgatcac ggtgaccgcc ttcaatgcgc agtcgcctcc cgggaagatt ttcgggggaa
    15841 cgctagtggg ggtgaacctc acagccggcg caccggtcaa tctggcaatc acgctggttc
    15901 ggctcttcac ggttaccgtg caaaaagaag gggagggcag cggggcggtc acttccacac
    15961 ctgggggcat cgattgcggt gtgacctgtg taaatcagtt cccagagggc gccacggtgg
    16021 tcctcaatgc tgccgcggca cccggttctg cgtttgccgg ctggagtggc ggttgttccg
    16081 gcacgggggc ctgcagcgta acggggaatg cgacggtgac ggcacgcttt actgtcccgg
    16141 tttccaccga tcgactgacc gtgaccctgg ggggaaatgg aaccgggacc gtcaccagta
    16201 atcccagcgg gatttcctgc ccgggcacgt gcgccgccga ctttgccacc ggcatcaccg
    16261 tgtccctcac cgcggcgccg actaatggat ccacgtttaa tggattcagc ggcgcgggct
    16321 gcagtggttc cagctcgtcc tgtaccatcg ccataaatgg cgatcaggcc gtcaccgcga
    16381 cgtttacggc agtgatccca attcccacgt ccactctttc cgtgagtcgg attggatcgg
    16441 gagtcggcac tgtgacgagt acgaatcaag cgggcatcaa ctgcggaagt gtctgcagcg
    16501 ccagcttccc aaccggaagc gtggtctcat tgactgcgac gccatcaaca ggctcgacgt
    16561 tcatcggctg gagcaatgga tgcaccggga ccggctcctg cgacgtgacc ctgaacgccg
    16621 atcaaactct cgtcgcgcaa ttcgatctcg tgcctgacgt cgtgacgctg tctgtgaata
    16681 catccggacc gggcgacggg acggtgacga gcgatcaggg cggcatttct tgccctaacg
    16741 cctgttcggc ttctttcacc cgaggaacga cggtcgtgac tttgaccgca acaccggtcg
    16801 gaaactcggt tttcgcggga tggcgtggaa atccctgtga agcattcgga accggaccct
    16861 gcatgattac tatggacgat aatcggtcgg caaatgcccg attcgaacga ggtggcggtc
    16921 gttgagaagc ctgttcaaga agcctgcact tcgcgaaatc ccagcatgcg tcctgcagga
    16981 gcggcggtcc gatggttggc aattgtaatt gtccggttca gagaaggaag ggtaaacgat
    17041 gcgccgacct tgggggattg accgattcgg attatttccc ggatcgactc gccgagcagt
    17101 tctttggccg ttcgatcagg tgttccccga ggaaggaacg tctccaattc gtcaaggccg
    17161 aatttggaga ggccgaggct gtagcaccaa aacatatccg actgggcttg atcgtcctgt
    17221 gcgactagga cgtgctcgtc ggcgacgaat tcgataaggc tgcgatcctg ccaatcggag
    17281 ggattgagat actgctgggt cgtcaggtca tgagcggttc cctgtgtcag gagtaccagg
    17341 cctctggcaa gtcgcgccgc gaacaagatg gtatccggca tgtcgcgtgg gggcatcacg
    17401 gacggagcaa tggcgccgat ctgatcatgt tcccatgcca attggtcttt caggccgacc
    17461 acgtgcgttg agggaagcgg aatgacgaca tgcgcggtcc aaggtccgtg tctgatatgc
    17521 caggactcag gggccccgcc ttcgtggtca atcgtcaagg gcccgccata ttggccgttg
    17581 taccagaaga tgagctcctc tttttgcggt gccggcccgc gatagccgat gaaatacaac
    17641 ggcggtcgct tggcggaggg cttagggctt ttcttcttga tacgcatggg aagggcagcg
    17701 ctggcgcttt tgctgaacgg gtagcgaagc taggaacgca cggctcggat catactggcc
    17761 cgacggttac ttccccgatt ttttagcttt gcgccgatct tccggatgga gcagccgttt
    17821 acgaatacgc agattattcg gcgtgacttc cacgagctca tcctgcccga tgtactcgag
    17881 agcaaactcc aaagacattt cctgcggtgg agtcagcacc aaggcctcgt cagatcctga
    17941 ggcgcgcatg ttcgtgagat gcttttcttt gcagacattg acgtccagat cttcgtcccg
    18001 gctgttttcc ccgacgacca tgccccgata gacttccacc cccggcccga taaacatgac
    18061 gccgcgctcc tgcgtcatga acagcgcata gccggtgctc ttgccgtcct cgaacgcaca
    18121 gagggagcca tgcggcgcga caagtaaatc ctgttcgttg gccgtttcgt aggctttgaa
    18181 gacgtgatgc atgatcacgg tgccacgggt cttggcgaga aggatgttct tcaaccccat
    18241 gatccccctg gtgggaatat ggtactcaag atgcatttcg ctcgggccgg cgtctgaatg
    18301 caccagcttc atatgccgca tttctccgcg gcgtttgccg atttcctcga tgacggcccc
    18361 ttgataggtc tccggcactt gaatcgtaag ttcttcgtac ggctcggtga ccttgccgcc
    18421 ctcgcgatgc agaatgactt ccggttggga gacttgcagc tcgtagcctt cacgccgcat
    18481 ttgctcaatc aagaccgcca ggtgcaattc gccgcgtccc gccacgagga atcgatccgg
    18541 actgtccgtt tcctgtacgc ggagagaaac gttcgtttca agttctttaa agaggcgctc
    18601 gcggaggtgt cgggaggtca agaacttgcc ttcacggccg gcgaacgggc tgttgttcac
    18661 cgaaaaagtc atttgaacgg tgggctcgtc gatggtgacc cttggcagcg gtgagggttg
    18721 tacggcatcc gcgatcgtgt ccccgatgct cacttggtcc aatcccgcga ccgcaacaat
    18781 ctcgccggct ttggcttctt caacatccgt gcgctccagc cctgaataga ccgcgaggtc
    18841 ggataccttc ccgggagcgg cgccgtcctt ccccagaacc agtacttgtt gccgacgggc
    18901 gatcgagccg gactgaatct ttcccacgcc cattttgccc ttgtacgagt cgtagaggag
    18961 tgcgagcacc agtatttgca gcggcccgtt cgcgttgatc gccggaccgg gaattttctc
    19021 cagcaccgta tcgagcagcg gcgagatgtc cgtgccgggc ttgtttatgt cgagcgtggc
    19081 aataccgttg actgccgagg tgtagacgag cgggaagtcc agttggtcat cggtggctcc
    19141 gagatgcacg aacagatcga aggtgcggtt gaccacatcg tcgatgaccg catcaggccg
    19201 gtcgatcttg ttgatgacca caatggcctt gtgcccgagt gccagcgctt tcctgagcac
    19261 gaaggtggtc tgcggcatcg gtccttcttt ggcgtcgatc agaatgagga cgccgtccac
    19321 catgcgcagg gtgcgctcta cttctccgcc gaaatcagca tgaccaggtg tatcgacaat
    19381 gttgatttta acgcccttgt agatgacgct cgcatttttg gctcggatcg tgatgccccg
    19441 ttcccgttcc tggtccatgg tatccatgat gcgttcgccc atgtcatcga tctttcgatg
    19501 gacgtgggtc tgccgcagca gggcatccac gagcgtagtc ttcccgtggt ccacgtgggc
    19561 gatgatcgcg atgttgcgga tgtcgttgcg gcgttcgttt ggtacgcgta gggacatgtc
    19621 ctggtatcct aaggcgaaaa aaaagacgcc ccgactatcg ggcgtcgaca gggcagtata
    19681 ccggaagcac ggagcgccta caaggtggca gttgcttgtg ttttagcaga ctccacggta
    19741 tgttgagccg agagtccttc cgaatcattt ccatgacatt gatagatcgg ccagttcagc
    19801 tttccgatac gaccagacag ccgccccctc gacgtccctg gcgccggtgg catatcacgc
    19861 tgatcacgct ggcggcgctc ttcctcgccg cgctgctggc gactgtcggc ttgttgtggt
    19921 actacgccca ggacctgccg gagctcagcc agcttcagaa ctaccaaccc agtcttgtca
    19981 cacaagtgta ttccagcgac cagcaactga tcgggcaatt tttcatcgaa cggcgcattt
    20041 tgacgcccct ggcgcagatt ccagagcatc tacggcgtgc cgtgatcgct gtggaagatg
    20101 tgcgattctt tgaacatccg ggtttggatt acatcggcat ccttcgtgct gcctggacga
    20161 atctgcgccg cggcggaaaa gtggagggtg ccagtacgat cacacagcag ttggcgcggt
    20221 cgctattttt gtcgtccgaa cggactttcg atcgaaaagt ccgggagctg gtattggcct
    20281 acaagatgga gctcgtctca accaaagaac agattctcga aacgtatctc aaccaaatct
    20341 acttcggcca gggagcgtat ggggtcgcgt cagcggctca gtcctacttt ggcaaggact
    20401 tggctgggtt gaccgtcgcc gaagccgctt tcctggcagg attgccgaaa tctccgaatc
    20461 atttctctcc tttcaaaaat tatgcgcgtg ccaaaaagcg acaagaacat gtgctggagc
    20521 ggatggaaga agcgggtttt ctcacatccg cccagcgcga agaagcgtta gctgaaccat
    20581 tgagtttccg gcgccccggt agtgaacaga ccgctccgta cttcgtggag tacgtgcgac
    20641 aacatctcgt cgcgaagtat ggagaggcca tggtgtataa ggggggactc cggatcaaaa
    20701 cgacactcaa tctcggcatg cagcgtgccg ctgaagcggc cttcaagaca ggcttacggg
    20761 agctcgacaa gcgccagggg tggcgcggcc ccctgcgaac ggtagaccct gccaccctga
    20821 caacgggcac ccagccggtg gcaacgaccg atcagccggt ggccataggt gatgagcggg
    20881 aagcagtagt gaccaaatcc ggcaaagacg tattctgggt gcagatcggc aatgtctccg
    20941 ccaagctcct atttgatgac atggcctggg ctaaaagacg cctgacagga ccggatctgg
    21001 cgaaggatat cgccgtggca tccactccaa agcaggtgct aaagccgggc gatgtcatcg
    21061 aggtcaaaat caaaaagatc gacaaagagc aagtgtatgt tcaactagaa cagactccgc
    21121 tggttgaagg aggactgatc gccctcgatc cggataaagg cgcaatccgc gcgatggttg
    21181 gaggctacga ctttaatcgc agcgaataca accgcgctgt tcaagcccat cggcaaccag
    21241 ggtcggcctt caagccgatt atctatgcga cggccatcaa tcaagggatg agccctgcct
    21301 ctgtcgtgct cgacgcgccc gttgtctatg agcaggtcga ggaggagaag acctggaagc
    21361 ccgagaacta tggtaaacgc ttccacggga tggtgagtct tcgcgacgcc ttggcccatt
    21421 ctcataatct ggccacagtc agattgcttg ataaggtggg cattaagaat gtcatcgagt
    21481 ttgcgcggca ggtcgggatt accagtccgc tgtctgccga tctctctttg ggtttggggt
    21541 cttcttcggt cggcttgctt gaactgacgt cggcctatgg cgtgttcttg aaccaaggca
    21601 atcgagcaga accgtttgcc atcatctcgg taacggacaa tgcgggcgag gtcctggagc
    21661 aggaggacgt gcagacacaa gaggtcctat ccaaagagac ggcctatctc atcacgaaca
    21721 tgatggagga cgtggtgcaa aaggggaccg gccaggcggc taaatcgctc ggcaggccga
    21781 ttgccggcaa gacaggcacg acgaacgaat ttatcaatgc gtggttcatc gggggaactc
    21841 cgaatctcgt tgcgggaatc ttcgtgggct ttgacgatcg gcgttcgctc ggcgaaaccg
    21901 aatcaggggc tcatgcagcc ttgccgattt ggattacatt catgaaggag gcgctgagga
    21961 ctctaccggt cgtcccgttt acgattcccg agggaattac gttcgtcaag gtcgatccct
    22021 caacgggtct cctcaatacg gagcaggagg atcagccggg aaacgtcgaa atcttcgcga
    22081 gaggaagtga accgacccag gcggtgcagc gccgttccga tccgatggac ttctataaaa
    22141 tcgaccagcc agagtagaga gaggagggag tgtcaggatg ccgagtcttg atattcctcg
    22201 tgccaagcca tttgaatcgt ttccagaatc ttttcattgg attttgtcgg gtcgtccttg
    22261 aaatccggca aggcgacgat ccacgcatgt aggtcggtaa atcgtacggt caacggatct
    22321 gttcccgggt gttcttcgac gagcctgatc gcgatatctt ctgtgtcctg ccatttcagg
    22381 tccatgtaca actcctcgcc tgtggcccta tcaaacgtct ttccggtggc cgaatctcaa
    22441 tcacgttttg agacttcgga gacttttttc cctttcaagc tccgtttgag tgaatcatca
    22501 agcatggcga ccgacagcgg tttggccgcc tgctcgagtt cggccatccg tgcccgaatc
    22561 gccttcgggt cgttgccatc ggatgcggct ttgagtagag atagtctgtc ccgaatggct
    22621 gcggcttcct cccgcccgat gagcttgtca ccgtcggcca gtgacttttc ggtagtcgtc
    22681 atgagggcgc tggcctcgag ccgcgcttca atgagcttcc tggcgtcgac gtcttgctgc
    22741 gcgaacttga acgaatcttc gatcatccgt tcgacttcgg tgtccgataa gccataggac
    22801 ggtttcactt cgatcgattg ggtctggccc gtccgcatgt cgcttgcttt gacgttcaaa
    22861 ataccgtttg catcgatcaa aaacgtcacc tcgatccggg ggacgcccgc cggaagcgga
    22921 gggactttga ggcgaaatcg tgcgaggctg cggttatctt tgacgagctc gcgttctccc
    22981 tgaagaatgt gaatatcgac accggtctgg ccgtcaacgt atgtagtaaa caattccttg
    23041 gcactggctg gaatggtcgt attccgtcta atgagactgc tcatcacgcc gcccatcgtc
    23101 tcgatgccga gcgacaacgg cgtcacatca agcagcaaca tgtcggtcgt gccgccggta
    23161 agaatgtcgg cctgaacggc agcaccgagg gcgaccactt catcggggtt caaatgacag
    23221 tgaggctttt tcccgaagag tgactctacg cgctgacgca cgagcggcat acgagtcgat
    23281 cctcccacca acaccacttc gtcgatgtcg gcaggcgaca gccccgcgtc ttgtaaggcc
    23341 aatcggcagg gcgacagcgt ccgttcgatg atgtccgccg tcaccgtttc cagctggtcc
    23401 ctcgtcagtt cacgggtaaa gcggcccttg ccgtcaggaa ggtcgatcgc aatgttggtt
    23461 ttgagttcgt ccgacagccg gatttttgcc cgttccgcct cgagacgtac gatctgcatg
    23521 tggtccgggt atgcactaag atcgatctcg aacgccttgc gaatctcggt caggaagagg
    23581 tcagcgaccc ggcggtccac atcatcgccg cccaagtgcg tgtcgccgtt ggtggcgagg
    23641 acttcgaaaa tgccgttctt cagcttgaga attgaaatat caaaggttcc gccgccaaag
    23701 tcatagacgg cgatcgttcc ctgagttttc tcttggagcc cataggcaag cgacgcggca
    23761 gtcggttcat tgatgatgcg caacacttcg agaccggcga tcatgcccgc atccttcgta
    23821 gcctgccgtt gactatcatt aaaataggcg gggactgtaa tgaccgcttt ggtaatgctt
    23881 tcgcctagat gtgactcagc acgctgcttg agctctttca agatcatggc tgagatttgc
    23941 ggcggggaat aggatttttc tcccagctgg atgcggatca cgccgccctg ttctgtcagc
    24001 gagtagggaa agtaggacag ttcactctgc acatctgcca aacctttccc catgaaccgc
    24061 ttgacggagt agacggttcg cgcggggttg cgcgtcagat gctccttcgc cgagtctccg
    24121 acgatgaggc cattatcggt tagtgcgacg acggagggca ccatcgtccg gccgcttcga
    24181 ccggcgatca cggtcggcgt gccatccttc atgtaggcga cgagggagtt cgtcgttcca
    24241 agatcaatcc caacaatgcg tgccatgtgt ttatccgatg gtcgcgacaa ggtcgctggc
    24301 gatgtttttg acgtaggtgc gattcgagag gatttcccgc atctgcttca ggagacgctc
    24361 ccgatcggcc cgtgccccgc ttgtagcctc ccccttttct tgaaggtcgt cccactggcg
    24421 aaagagctgg tggagctgct cttccatttc acgcttgcga cgctcaagcg cagcttgtgc
    24481 ggtttgcagc tgtgtccgaa gctgctttcc cgtctctgtc gactgatcgg aggttctaaa
    24541 ttcttcgagc gtatcctgga gttcgagaat ctcctcaaac aggtcggccg ggggcgaact
    24601 gcggatttcc ttcacggatc ccgcttctag gtctagcaga tactcagcgc gttgaatcgg
    24661 atctcgaagc gtccggtatg cggtattgag ggtcgccgcg ttactgaggc tgatggcctg
    24721 ttcagtctcg ctcttgttct gatagaagtc aggatggaat gcccggctga gctcgtaaaa
    24781 cttggcttcc aacgtcccga gatcgatcgt caggcggcgc ggaaagccaa ggcatgtgaa
    24841 gtaatctgct tctttcgaga cgggctgtac cttgacacac cgttcgcaga aatattcacc
    24901 ggccacctcg gattggcagt gccagcacat gcttctggcc atctgcaagt cccggcgccc
    24961 cgaattgtaa ctgccagatt gatgatccat gggcacatgc tgatctgaaa aaagaggcat
    25021 ggcggaatgc catgcctgat cgaagaaact cggacttggc ttcaagctta tgccgagaac
    25081 gattctccgc agccgcaggt cttgttggcg ttgggattga cgaacttgaa gtttccgccc
    25141 atcaggtcct tctgataatc gagttgcgtg ccttgaaggt agatggcact tttggtatct
    25201 acgatgacct tcacaccttc aaaatcaaag acctgatcgt attgaccaat cttttcgtcg
    25261 aaattgaccg tgtaactcaa tccagagcac ccgccgcctt tcacgccaag acgaagcccg
    25321 ccctcagtga taccttgaac attaatcaga cgtttcactt ccttgattgc cgcatcggtg
    25381 agcgtgatga cgggtacctg ggtctctgtc ttcgtgctat ccatgaggcg ctccttcctg
    25441 cttacttcgt ttcgctcttt ttctggtagt cggcaagagc cgccttgatc gcatcttcag
    25501 cgagcaccga acaatggatc ttcacaggcg gcaggttcaa ctcctgcacg atgtcggtat
    25561 tcttgatttt ttgggcctct tccagcgtct tccctttgag ccattcagta gccaggcttg
    25621 aactcgcgat agcggatccg cagccgaacg tcttgaattt cgcatcaacg atcgtatcgt
    25681 tctgaacctt gatttgaagt ttcatgacgt cgccgcattc cggcgcgccg accattcccg
    25741 tgccgacgcc gtcctcttcc ttcttaaagc ttcccatgtt ccggggatta ttgaaatgat
    25801 cgacgacctt gtcactgtat gccatagtac cctccgcatt tatgagatct agacacgtaa
    25861 gcttcttcta tcctctatca gtgagcagcc cattgaactg atttcaaatc caccccttct
    25921 ttcgccattt cataaagtgg agacatctct cgcagcttcg tcactatttc aataaccttc
    25981 ttgatcgtat agtcaatttc ctgatccgtg ttgaagcgtc ccagaccgaa gcggatggat
    26041 gaatgggcca gttccgttcc gacgccgagg gcgcggagta cataggaggg ctccaacgtg
    26101 gcggacgtgc aagccgaccc ggaggacaac gcgatttcct tcatccccat caacaaggac
    26161 tccccttcaa cgtaggcaaa ggaaatgttc aaattacccg gcagacgatt ggttgggtgg
    26221 ccgttgagat agctttcctc caaggcggcc atgatcccag cttgcaggcg gtcacgcatg
    26281 ttcgccaagc gggctgattc ggcgggcatt tcctgctcgc agagttcaca ggccttgccg
    26341 aatcccacga tcaacgggac gggcaacgtg ccggagcgca tgccgcgctc gtgcccgcca
    26401 ccgtcgattt gcgcggcgat ccgaacacgg gggttctttt ttctgacgta cagcgcgccg
    26461 acgcctttcg gaccataaat cttatgcgca gagaatgaca tgaggtcgat gcccatggcc
    26521 tgaacgtcga ccgggatctt gccgacgccc tgtgtggcgt cgcagtgaaa aagcacgccc
    26581 cgttccttgg caattttgcc gatgtcttga atgggattga tcgtgccgat ctcattgttg
    26641 gcgagcatca ccgagatgag taccgtcttt tcggtgaggg ccttccgcac atcctctgga
    26701 ttgaccatcc cctgcttgtc gacgggcagg taggtgacag tcgccagacc tttggcctcg
    26761 agtgatttgg ccgtgtccaa gaccgctcga tgctccgtcg cggaggtgat gatgtgattc
    26821 cccttttctt tgtacatttc cagaacaccc ttcagtgcga gattatcgga ttccgtcgca
    26881 ccgctggtga agacgatttc ttttggatcg gctttaatca ggcgggcaat ctgcttgcgc
    26941 gctgcctcaa cagcctcttc tgccgcccaa ccaaacgcat gattacgact agccgcattg
    27001 ccaaacttct cgacgaaata cggaagcatg gcctccaaca ctcgcggatc catgggcgtg
    27061 gtggaatgat tgtctaaaaa aatagggaac ttcatcgttc ggctccttgt gatgtcggcg
    27121 actgaatcgt aatgagtggg gtgccgccca acatgtcctg aagagtcatg ttgttcagca
    27181 actgataaat actgtcttgc accttcaaaa gaggcgtacg gatattgcag tgttcccgtt
    27241 gcatgcagaa ttcaccctct ttttcgtggg aacagtcggt aattccgagg ggcccttcaa
    27301 tactttccag aatttgggca atggttattg ctcgtgcgtt gcgtgcgagg tggtaccccc
    27361 cctttgggcc gttatggctt tcgatcatgc cgctcttgga gagtgattga agtactttgg
    27421 caaggagttc caaggggata ttatattcct cggcgatttc tttcgtattg accacccgac
    27481 ccggtgtcac gtcgccaaat tggaccgacg caatgtgctg aagggccatg agcgcataat
    27541 cggttttttt tgaaatcttg agcatgctat ctattgccga tcggtctagt cggagactat
    27601 aatgatctcc gatcaatctg gtcggactaa tggtagcacg gggaatttag acctgtcaac
    27661 acgggcttga gaaaggaatc aggtatgggt ggaaccaatc cctatataga aaaggcggac
    27721 gttgagctcc cgcaaaaacc gtatacagtg acctacgttg cgccgagtgg cacagtcacc
    27781 aaagtacagg tcgacccggc gaggatccct tacggccaaa caggactccc aggcagcctc
    27841 ctcgacatcg ctatggcgaa cggggtggat cttgagcacg tgtgcggtgg cgtctgtgcg
    27901 tgttcgacct gccatgtcat cgtgaagcag ggattggaaa cctgcaacga gggcacggac
    27961 gatgaattcg atcaactgga tgaggcgccg atgaccaccc ttcagtcacg tctgggttgc
    28021 caatgcgttc ccaacggcac gaaggatgtc gtcgttgaaa ttccggctgt caataagaac
    28081 ctggtgaaag aagggcatta gctgctcgct tccaggctgt ttgactccgg ggcgtcagtc
    28141 gaaggtttcc gttttcacct ctttccggtc atagccggct tcaaggaagt gagcccgcag
    28201 tggtttcaca aaggcttttg ttccacagag catcggaagc acttgtgggc tagcctgcag
    28261 caagggacgc agcatcggca gtgtcaggtc tacagcctgt tgttcacctc ccgccgcgac
    28321 caagggcaag tagcgaaaac cgggatgagt caccgccaat gtgaggagtt cctgatgaaa
    28381 cagcagctct tgttcctcgg gggcaatggc gatgaggagt accggaccgc gatatcgttc
    28441 agcatacagg tgtttcagca tgcaacggat gggaaccaga cctgtgtaac gtgtgacgaa
    28501 caacaactca cggtcgatct gcggcggaag caaaaaattt ccataggggc ccgacaggat
    28561 gagctcgtca ccgggcttcg ccaagaacag gtaattggac cccaatcccc ccggtacccg
    28621 gtcaaggacc aggattaatt cacctgatgt cgtaccggga gccgccatcg aataggctcg
    28681 attgagaggc ggctttatcc cgaccggtag cttgagtgat acccactggc ccgccttgaa
    28741 gacgatagac tgttctttcg gcaacaccac gagttgacga acgtgaggcg tgagattcgt
    28801 cgacgacagg acagttgcgg gttggataaa ttctgccatg gcgaggccag tggtgctcag
    28861 tcttatcaga tgcccgggtg ggaagaaagg gcgttcgatc ctatacagag ctttggacgc
    28921 gtgctataga taccggccct cgaccacgat tagcgagagc aattatgacc caagttcgtg
    28981 tccgttttgc gcctagtcca acaggtttcc tgcatatcgg cggtgtacgg acggcgctct
    29041 ttaattggct gtacgcgagg caagagcacg gtgtcttcat cctgcgcatt gaagataccg
    29101 accagagccg gtcgacggat gaatccatcc aagcgatcgt tgaaggattg aaatgggtgg
    29161 ggcttgattg ggacgaggga ccgtatcggc agaccgagcg gatgaccctc tatcgtgagc
    29221 atgcgatggc cctgttcgag cgaggacaag cctactggtg catctgtaag gccgaagagc
    29281 tggaggcccg gagaaaagag gccgagggga aaggtctgtc gcctcgctat gacgggcggt
    29341 gccggacgtt ggggatcact catccttcgg gagatgccgc gctgaggttc aaagcaccac
    29401 aggaagggca gaccgtggtg gacgacttga tcaaaggcaa agtcgtgttc gagaataacg
    29461 tgctcgacga cctgattatc ctgcgatcaa acggatatcc gacctataac ttctcggttg
    29521 tagtggacga tgcgttaatg cacatcaccc acgtcatccg aggggatgat catctcacga
    29581 atacaccacg gcaaattccc atctttgaag cgctcggatt tcctgtgccg cgcttcggcc
    29641 atctgccgat gattctaggt gccgataaga cgcggctctc caagcgacac ggcgcgacct
    29701 ctatcatggc ctataaaacg atggggtacc tgcccgatgc gatggtcaac tatctagtcc
    29761 gactcggatg gtcgtacggc gatcaggaaa tcttcaccag gcaggagctg atcgagagat
    29821 tcagctggaa gcaggttcag acttctgcgg ccgtcttcaa tcccgacaag ttattgtgga
    29881 tgaatgccga gtacatcaaa acgagtccgc cgagcgaggt ggcccaagca ctggtgccat
    29941 acttggagca gacagacctg aaggatcaac tgcaggaagt ccccaaggag tggcttgcgc
    30001 agctggtgct cttggtcagg gagcgcacaa aaacgctcgt ggaaatggtc gactgggtga
    30061 agccgtattt tggagaagcc gtgacgttag aagaggaagc ggcaaaaaaa tatctgaccc
    30121 aggcggtcgc ccctgttttg aaaaagctaa ccgaccgtat cgggtctctt ccagaatttt
    30181 caaaaccggc attggaaggg tgtttcaaaa ccctcgtgga agaggaggga ttgaaaatgg
    30241 ggcagctcgc tcagcccgtc cgagttgcgt tgacgggtcg tactgcgagt ccaggactct
    30301 tcgaagtgat ggaaatcctc ggccccgaac gaacccggct gagacttcga caagggatcg
    30361 agcgggctga ggcccgccag gcttgacgga ctacgcggac gtgtattacg ttgaacgccg
    30421 gttgggggat cgtctagcgg taggacgtca gtctctggat ctgactacct aggttcgatt
    30481 cctagtcccc cagccaccgg ctcctcacat tcaccgcctt cactctcggc cgcgctgggg
    30541 gatcgtctag cggtaggacg ccagcctttg gagctggcta cctaggttcg attcctagtc
    30601 ccccagccac aatttcttta aagactaggc agccaacgga cggccggtga tgtcttgttg
    30661 ggttgccact tcaatggaag agacccgttc gtcccgccaa agcgcaatgc cgcctatcag
    30721 gccattgaga tttgaaataa tcgtgacatt ggcgggcagc atgccgacat cgacggctcg
    30781 ggaattgccg ccaccgatat acattttcgt aaaatgaaag atttcatcga ggcgtttaat
    30841 aaatttcacg aggcgcttgt tccacctttt cttacccact ttttgtaagg cggcgtcccg
    30901 cagtttatcc cggccgattt cgagattagg cacgaggcgt ccatccacaa agagggaagt
    30961 tcccacgcca gttccgagcg tcacgaccag ttccacgccg gtgccggaga tggcgccaaa
    31021 gccttgcaca tcggcatcat tggccacccg caccggtttt ttcagtcgcg cttccagtac
    31081 ttcagcgata ttgtagtcct gccattcctt agcgaaattc ggcgcttg
//
LOCUS       DASSCQ010000025         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_192433, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000025 DASSCQ010000000
VERSION     DASSCQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192433"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..162)
                     /locus_tag="VFH05_01850"
     tRNA            complement(76..162)
                     /locus_tag="VFH05_01850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Leu,seq:cag)
     gene            complement(334..1776)
                     /locus_tag="VFH05_01855"
     CDS             complement(334..1776)
                     /locus_tag="VFH05_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676986.1"
                     /translation="MTARELIEFWTTRLTAEHHRLGDSETDRPITPVTGRLVQTVGTL
                     YLYEFKLPPDRSVIIDTPVSIVPDDGMEPTEGYVLSHRDGVALVQTFDAVGSAIANAT
                     IVPDRGSFLMAAVERFAQMLSQPDSYRLGPADRLAHVFEASEPWSSSSASVLTTMWSE
                     DPALRQQKIAATAIELIRTNKRILLVSPDHEAADAISGVIAKAMKAGGLVYKTWVSRY
                     EMTLTHHASGLPIHELGFEAQMHQFYAKSRADKAALRRKYERFRELTPILAYKGHKQK
                     DLDEVRLLEWRLLTQFSDLQAKSQEARATLEAYETLPLLRRLTMQAVGKNVESLHQYL
                     ALYEGQMAALRKELEIAKARIDELVPEAAVPKDMRPEFEDLKEEIVRLGGTKKIRELL
                     AAEEDTNRQAFIQNRRLVVTTADRVLGDPLFSRVRFDVLIAENAPWISPHALLGAAGL
                     VRERIVLTGDQRDISTAGVWKSVQTAGSQR"
     gene            1974..2441
                     /gene="rplI"
                     /locus_tag="VFH05_01860"
     CDS             1974..2441
                     /gene="rplI"
                     /locus_tag="VFH05_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET6676987.1"
                     /translation="MKVILQETMEGVGHLGDLIDVKDGFARNFLLPRNKAVVANDRNI
                     KSFEHSKRVAAERAKKEKLEIEAHAKKISTVTLTVEAQVGKDDKMFGSVTVKDIAEGL
                     AGQGYTVDRRKIQLPHPIKELGSFTVPVKLPREVTAAVTVRVVKKQEPETATT"
     gene            2460..3728
                     /gene="glyA"
                     /locus_tag="VFH05_01865"
     CDS             2460..3728
                     /gene="glyA"
                     /locus_tag="VFH05_01865"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET6676988.1"
                     /translation="MSMKDAVGSLDALQTTDPQVYAAIDAEEERQRNKLLLIASENFA
                     SPAVLAAQGSLMTNKYAEGYPGKRYYGGCQHVDTVEELAIQRCKQIFGAEHVNVQPHS
                     GSQANMAAYLSVLKPGDTILGMDLAQGGHLTHGSKVNFSGMIFRAFSYGVDRQTETID
                     YDAVRKLAEDCRPRMIVVGASAYARTLDFPKFAAIAKGVGAYLMVDMAHIAGLIATGL
                     HPNPIPYADFVTTTTHKTLRGPRGGVVMCKAEHAKAVDKFVFPGMQGGPLMHVIAAKA
                     VAFKEALSPAFKRYQQQVIANARVMAQGLLDRGYKIVSGGTDTHLMLVNLTNKGITGK
                     EADAALDTAGIIVNKNAVPYDEKPPAVASGIRLGTPIVSTRGMKEAEMKEIVDLVDRV
                     LQHRHDTAVLEQIRGQAKALCSRFPIFHSY"
     gene            3755..4219
                     /gene="nrdR"
                     /locus_tag="VFH05_01870"
     CDS             3755..4219
                     /gene="nrdR"
                     /locus_tag="VFH05_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET6676989.1"
                     /translation="MKCPFCDELEDKVVDSRMAKEGELIRRRRECLGCKRRYTTYERV
                     EEILPVVVKKDGRRETFDRSKILSGLKKACEKRPISTATIEAVADRIEKHIQEMGETE
                     IESRVVGEELMKELHQLDQVAYVRFASVYREFKDIDQFMDELRTLAQQRRER"
     gene            4306..5415
                     /locus_tag="VFH05_01875"
     CDS             4306..5415
                     /locus_tag="VFH05_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6676990.1"
                     /translation="MPASQTKLSKQTRKHGVAGVTHVAIIGAGRGGTALLEIFANDPL
                     VDIVAVAEIDASAPGLALAKRFKIPVTRDYRMLLNLERVDLIIDVSGSPDVWQSLQDF
                     HRMGVTILGGASAKFMWELIEARIRATAEIEKTLNKYQSLYRLYVKETGAAVTEERTR
                     IACEIHDGLVQSLAGVGFKLELSQQLIRKDPKASLATLRESKEQLKHAIHEARQVIFN
                     LRPLQYDKMELIPGLTNYLKSYETQYHIKTEFTVSGDEQILFPRTKIFLFRIVQEALS
                     NVVQHAKADRVSVRLTIDLDILRVTISDNGVGFDMQHVLHNPAKWDHFGVRGILERAK
                     LVGGEAKIDSKKGRGTKIVVEVPLGKKEHSNDGKN"
     gene            5402..6073
                     /locus_tag="VFH05_01880"
     CDS             5402..6073
                     /locus_tag="VFH05_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6676991.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKDDINVVGEAQDGMEAVEK
                     TKTLMPDVILMDVSMPRMGGVEATRQIKREFPHIGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMAQKKGKKPGWVEHDLTER
                     EITVLRLVADGKTNKEIANSLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LDPRP"
     gene            6186..>6467
                     /locus_tag="VFH05_01885"
     CDS             6186..>6467
                     /locus_tag="VFH05_01885"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET6676992.1"
                     /translation="MQHTAADWWSQLRIQHKVWAVLLLLCLPLIAGLSTHLYLVQQLL
                     TLQEHRHDLILAHEQVNLLRRLAVDIEDGFRGYLLTQQSAFLAPFAEAES"
BASE COUNT         1501 a         1825 c         1804 g         1337 t
ORIGIN      
        1 aaccgcgaat gagatgatga gggtgggcgc ctaatgccaa ctcttctcag taatatagag
       61 agtcacataa agagctggtg ccgaagccgg gatttgaacc cggacaccct tgcgggcgct
      121 agaccctgaa tctagtgcgt ctgccagttc cgccacttcg gcataggcac gctcaaaggt
      181 gcttcaacgc cattcagtcg cctgattcac cgcaacgtcg gtgggtttgc aggcttccgt
      241 ttgttgaacc acttcgcgcg ccaaacatgg atacgacagg gaacgcgatt atcctgaatt
      301 cgactcgctc aggtcaagca agcttccagg gatttaacgc tgactccctg ctgtttgcac
      361 ggatttccac acaccagccg tcgaaatgtc acgctgatct cccgtcagga caatccgttc
      421 gcgtaccagc ccagccgccc ctaacagagc gtgaggggaa atccatggtg cattctcggc
      481 gatgaggaca tcgaatcgga cccggctgaa cagcggatcg cccaatacgc ggtctgcggt
      541 cgtcacgacg agacgtcgat tctgaatgaa cgcttgtcta ttcgtgtctt cttcggcggc
      601 caaaagctcg cgaatttttt tcgtcccgcc cagccggaca atctcctcct tcaggtcttc
      661 gaattcaggc cgcatgtctt tgggcaccgc agcttcggga accagttcgt caatgcgagc
      721 cttggcaatc tcgagctctt ttcggagggc ggccatttgg ccttcgtaga gggcaagata
      781 ttgatggagc gattcgacat tcttgcccac cgcttgcatc gtgagacggc gcagcagcgg
      841 taaggtttcg tacgcctcca gcgtggctcg cgcttcttgg cttttggcct gcagatcgct
      901 gaattgggtg agcagtcgcc actccagtag ccgcacttcg tccaaatcct tttgcttgtg
      961 ccccttgtag gccaagattg gcgtgagttc gcggaagcgc tcatatttcc gacggagcgc
     1021 ggccttatca gcacgcgact tcgcatagaa ctgatgcatt tgggcttcaa atcctaactc
     1081 atgaatcggc aggcccgacg cgtgatgcgt caatgtcatt tcataccggc tgacccacgt
     1141 tttatagacc aagccaccgg ccttcatagc cttcgcgatg acgccgctga ttgcatccgc
     1201 cgcctcatgg tccggactga caagcaaaat ccgcttgttc gtgcggatca gctcgatcgc
     1261 ggtggccgca attttctgtt gccgcaaggc tggatcctcc gaccacatcg tcgtgagaac
     1321 ggaggccgat gaactgctcc acggctctga cgcctcaaag acgtgagcca gccggtctgc
     1381 aggtcccaat cggtatgaat cgggctgcga caacatctgc gcgaaccgtt caactgctgc
     1441 catgagaaag cttccccgat cgggaactat tgtggcgttg gcgatggccg atccaaccgc
     1501 atcgaacgtt tgcaccaagg ccactccatc ccgatggctc aacacgtaac cttcagttgg
     1561 ttccatgccg tcgtccggaa cgatagacac cggcgtatcg atgatcaccg aacgatcagg
     1621 cgggagcttg aactcataga gatacagggt tccgacagtc tgcaccagcc gcccggtgac
     1681 cggcgtaatc ggtcggtccg tctcggagtc gccaagccgg tggtgctcag cggtcaggcg
     1741 tgtggtccag aattcgatga gctctcgggc ggtcatgatc gtgtggtctc aacggatcca
     1801 gcggccatct tacgggcgcc agacgacgag agtccagccg ttgaacgtgg ccgtcaccgc
     1861 gtcgagccct gtcgccaacg aggcaaaact ccgtcagttc agtagatctg caattgactt
     1921 cctttggccc ctccagtatc ctcttttcct ttccaacaga aggagttaga ggcatgaagg
     1981 tcattttgca agagacaatg gaaggggtcg gccatctggg cgatctcatt gatgtcaaag
     2041 atggatttgc gaggaacttc ctgcttccac gcaacaaggc agtcgtcgcc aatgatcgga
     2101 atatcaaatc gttcgagcat agcaagcgtg tggcagctga acgggcaaag aaggagaagc
     2161 tcgagatcga agcgcacgcg aagaagattt ccactgtcac cctgacggtc gaggctcaag
     2221 tgggcaagga cgacaagatg tttggctcgg tcacggtcaa agacattgcc gagggtcttg
     2281 cggggcaagg ctacacggtc gatcgccgca agattcagct gccccatccc atcaaagagc
     2341 tgggcagctt caccgttccg gtgaagttac cgcgtgaggt gaccgctgca gtcaccgtgc
     2401 gcgtggtgaa gaaacaggaa cccgaaaccg ctaccacgta acaccaacga ggcgccttca
     2461 tgtccatgaa agatgcagtg ggctcccttg acgccctaca gaccaccgat ccccaggtct
     2521 acgccgccat cgatgccgaa gaggaacgac agcgcaacaa gctgttgctg atcgcctcgg
     2581 aaaacttcgc cagtccagca gtgttggccg cgcaaggctc actcatgacg aacaaatatg
     2641 ccgaaggcta tcccgggaaa cgctattatg gtggctgcca gcacgtggac accgttgaag
     2701 aattggccat ccaacgctgc aaacagatct tcggtgccga acatgtgaac gttcaaccac
     2761 attccggttc tcaagccaac atggcagcct atctctccgt cctcaaaccg ggggatacga
     2821 ttctcggcat ggacctcgcg cagggcggcc acctgactca tggaagcaaa gtgaatttct
     2881 cggggatgat tttccgtgcc ttttcctacg gtgtcgatcg ccaaacggaa accatcgact
     2941 acgacgcagt ccgcaagctg gccgaagatt gtcgtccacg gatgatcgta gtgggcgcga
     3001 gtgcctatgc gaggacgctc gattttccta agttcgcggc cattgccaaa ggagtcggcg
     3061 cgtatctcat ggtcgatatg gctcacattg ccggactgat cgccacgggt ttacatccca
     3121 atcccattcc atatgcggat ttcgtaacga cgacgacaca caagacactt cgcggcccac
     3181 gaggcggggt ggtcatgtgc aaggccgaac atgcaaaggc cgtcgacaag ttcgtctttc
     3241 ctggcatgca gggtgggccc ttgatgcatg tgatcgcggc caaagccgtg gcgttcaagg
     3301 aggctctttc tccggcgttc aaacgctatc aacagcaagt gattgccaat gctcgcgtaa
     3361 tggcgcaagg cctgctcgat cgaggctaca agattgtttc cggtgggacg gacacgcatc
     3421 tcatgctggt gaatctgacg aacaagggca ttaccggcaa agaggcggat gccgcgctcg
     3481 acacggccgg catcatcgtg aacaagaacg ccgtgcctta tgacgaaaag ccgcctgctg
     3541 tcgccagcgg tatccggctt gggacaccca tcgtgtcgac ccgcggaatg aaggaagccg
     3601 agatgaaaga gatcgtcgac ttggtcgacc gagtcttgca gcatcgacac gatacagcgg
     3661 tgctcgagca gatccgcggc caggcgaaag cgctgtgtag ccgttttccg atttttcact
     3721 cctactaact ctcacgcagg caggatcacc gccggtgaaa tgtcccttct gcgacgagct
     3781 ggaagacaaa gtagtcgact ctcgcatggc gaaggaggga gaactcatcc gacgccggag
     3841 agagtgtctc ggatgcaagc gccgttacac cacctatgag cgcgtcgaag agattcttcc
     3901 cgtcgtggtc aagaaagacg ggcgccggga aacgttcgat cgctcaaaaa tcctttcggg
     3961 gctcaagaag gcttgcgaaa agcgcccaat cagcacggcg accatcgaag cggtggccga
     4021 ccggattgaa aaacacattc aggaaatggg cgagaccgag attgagagtc gcgtggtcgg
     4081 cgaggagttg atgaaggaat tgcaccaact cgatcaagtc gcgtatgtgc gctttgcgtc
     4141 cgtatatcga gaattcaaag acatcgacca gttcatggac gagctgagaa ccctggccca
     4201 gcaacgtagg gagcggtgag gtcgctaccg gtctggtcgc tcggacgtcc ttcctctcct
     4261 cccgctcgtc cagatcatac acgctccaac agtgatagag ggcacatgcc ggcctcgcag
     4321 accaaactgt ccaaacagac caggaaacac ggagtggccg gcgtcacgca tgttgccatc
     4381 atcggcgccg gtcgcggcgg cactgcgctc ctggagatat ttgccaacga tcccctcgtg
     4441 gacatcgtgg ctgtggctga aattgatgcc tctgcgccgg gccttgcatt ggccaagcga
     4501 ttcaagattc ccgtcacgcg cgactatcgc atgcttctca accttgagcg cgtggatctc
     4561 attatcgatg tctcaggcag cccggatgtc tggcaatctc tgcaagactt tcatcgcatg
     4621 ggcgtgacga ttcttggcgg ggcaagcgcc aagttcatgt gggagttgat cgaagcccga
     4681 attagggcca ccgccgagat cgagaaaacg ctgaacaaat atcaatccct gtaccgcttg
     4741 tacgtcaagg aaacgggcgc tgccgtgacg gaagaacgga cgaggatcgc ctgcgagatt
     4801 cacgacggac tggtgcagag cctggccggc gtgggcttca aacttgaact ctcgcaacag
     4861 ttgattcgca aagatcccaa ggcaagtttg gcgacgctgc gtgaaagcaa ggagcagctc
     4921 aagcacgcga tccatgaggc gcgtcaagtc atcttcaatc tccgtccgct tcaatacgac
     4981 aagatggagc ttattccggg actcaccaac tatctgaagt cctatgagac gcaataccac
     5041 atcaagaccg aatttacggt gtcaggagat gagcagattt tgttcccccg cacgaagatc
     5101 ttcttgtttc gtattgtgca ggaggctctg agcaatgtcg tccaacatgc caaggccgat
     5161 cgggtgtccg tgcgactgac gatcgatctg gacatactca gagtcaccat ttcggataac
     5221 ggcgtcggct ttgacatgca acatgtcctc cataaccccg caaagtggga ccatttcggc
     5281 gtacgaggga ttctcgagcg ggcgaagctg gtcggaggcg aagcaaaaat cgattccaag
     5341 aaaggccggg gcacgaagat agtggttgag gtgccgctcg ggaagaagga gcatagcaac
     5401 gatggaaaaa attaaagtgc tcatcgcaga cgaccatcgt gtggtacggg aaggcctcgc
     5461 ggccattctg aagaccaagg acgacatcaa cgtcgtgggc gaagcgcagg acggaatgga
     5521 agcggtcgaa aaaaccaaga cgctcatgcc cgacgtgata ctgatggacg tcagcatgcc
     5581 tcggatgggc ggggtcgaag cgacccgtca gatcaaacgt gagttccctc atatcgggat
     5641 tgtcgcgctg accatgtatg aggaacagca atatattttt gatctggtcc gcgcgggggc
     5701 aaccggctat ctattgaaag attcagaatc atcgcaaatc gtcgccgcca tccgtgcgat
     5761 ctatcggggc gagtcattga tccatccttc cgtcgcgagc aagatcctcg ctgaattctc
     5821 cttgatggcc caaaagaagg gaaagaagcc cggctgggtg gaacacgatc tcactgagcg
     5881 cgagatcacg gtcctgcgcc tggtcgctga cggcaaaacg aataaggaga tcgcgaacag
     5941 tctcgatctc agcgagaaaa cggtcaaaaa tcacgtgcgg aatatctttc acaagcttca
     6001 ggtttatgac aggacacagg ccgctatttt ggcgatccgc aagggattaa tcgaattgga
     6061 tccccgccct tagatcgagc cattaggcac tagaaattag aaataactct ttgctaggtt
     6121 ggagcgtcct ctcccgctga accagggtct tcactcaggg tggccggcac gaaggacatc
     6181 gccacatgca gcacacagcg gcagactggt ggagtcagct ccgcatccaa cacaaagtct
     6241 gggcagttct tcttcttcta tgcctgcccc tcattgctgg actctccaca catctctatc
     6301 tcgtccaaca actgctcacc cttcaggaac accgccatga tctgatcctt gcccatgagc
     6361 aagtcaatct gctccgtcgt ttggcggtcg acatcgaaga tggttttcgt ggatacctcc
     6421 tgacacagca gtccgcgttc ctggctcctt tcgctgaggc agaatcg
//
LOCUS       DASSCQ010000026        13161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_727409, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000026 DASSCQ010000000
VERSION     DASSCQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727409"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFH05_01890"
     CDS             complement(<1..388)
                     /locus_tag="VFH05_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676993.1"
                     /translation="MAIVNQLRRQCALGCLVLTLGSFPPIVRAFDGDWPIEKLMALVA
                     EQRKPSVAFEEATYSSVLTEPLKVRGILRFIPPATLEKLVTDPLHERYVIQGDRVTFE
                     SQRTGVTRSLSLGEYPALRSFVEAFRM"
     gene            complement(461..1357)
                     /locus_tag="VFH05_01895"
     CDS             complement(461..1357)
                     /locus_tag="VFH05_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676994.1"
                     /translation="MAVAWKTRQERGNRRLIRLMAWVAESLGRPVARALLIPVCLYYL
                     CRSGEANQTLRQYYLRAQGRSPGWQGLFHHYYWFGSTILDRVYFLRGRFGLFDIRVNG
                     LDTLTRALSLGRGCLLLGSHLGSFEVVRAMGVSREELDIKVLMDEQNAPLMRAFSHEM
                     NPVVADSVLQVGGIDNMLRVRECLEGGGIVGIMGDRLMSHNQTVRCNFLGSDAPFPTG
                     SIRLAHAVRTPVVMFFGLYRGNNRYDVQLELLSEEIRLSSERREDDIRRWTQQYADRL
                     AHYSRNATDNWFNFYDFWQDTP"
     gene            complement(1357..1680)
                     /locus_tag="VFH05_01900"
     CDS             complement(1357..1680)
                     /locus_tag="VFH05_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6676995.1"
                     /translation="MTYERTLTINSDHPALPGHFPGHPVVPGVIVLEEVIQALREIHG
                     QGVVVTGMPAVKLSSPLRPEERLTITIESQESETAAFTCEVGTRLVASGCIRFRLPGG
                     SQSGS"
     gene            complement(1670..3013)
                     /locus_tag="VFH05_01905"
     CDS             complement(1670..3013)
                     /locus_tag="VFH05_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6676996.1"
                     /translation="MLDAPLIGSYRPDDVLAWESDGPRSAREFLSAVINLADGLPDRP
                     SVLNLLTSRYEFLVGFAAAMLRRQVTLLPQSRAPETLRRIAVEQESCYCLTDRGEPVH
                     DIPAVVLPAQRGSMRPWNIPRIPLHQHVAIAFTSGSTGQPTPNLKTWDALVSVARATA
                     KRLGVNAAGVKGVVATVPHQHMFGLEASVMLPVQQGLTMHAGRPLFPEDIRAALTESP
                     ERSLLVTTPLHLRACLLAGVRLPPIGMILSATSPLTSALAREAEQRFHAPVHEIFGFA
                     EAGSVASRRTVTDGDWHMLDGVALTRNGSHASIQAPYLPCPVPIPDRITVTGRATFRV
                     LGRAEDQVNIAGHRASLGDLTRQLLEVEGVHDGVFILPDAKEGSLSRLMALVVAPGKT
                     QEDIQHALRRRLNPVFLPRPLLLLPRLPRNETGKLPREALRQLMERWKDESSHDV"
     gene            complement(3027..3299)
                     /locus_tag="VFH05_01910"
     CDS             complement(3027..3299)
                     /locus_tag="VFH05_01910"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET6676997.1"
                     /translation="MISQQLTPFELEIAKLIVQALKLKADPSSIEPEAVLFGDGLGLD
                     SIDALELSLAIAQTYGYQLKSSDPEIKATFSSLRALARAIELHRTK"
     gene            complement(3296..4096)
                     /locus_tag="VFH05_01915"
     CDS             complement(3296..4096)
                     /locus_tag="VFH05_01915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6676998.1"
                     /translation="MNPESNHNIGTRSAGQLVPLPPHPLLRRYYDQDEARQRFLNDLF
                     NRTAPQYRNIDKATGLGSGLWYRRKALLRAGLTAGMHVLDVACGPGLVAQCARQIVGH
                     SGSVIGLDPSNGMLREARRGPCHTLVNAIGERLPFPDACFDFLSMGYALRHVSDLRHA
                     FSEYRRVLKPGGIVLLLDICRPRSPLLLSLSRFYVRTVLGIAFSASTGNRDMKTLMEY
                     WWDTTEHCVGADTIVAALKEAGFIECALHELFNGLLRDYRAVKPEAVL"
     gene            complement(4171..4863)
                     /locus_tag="VFH05_01920"
     CDS             complement(4171..4863)
                     /locus_tag="VFH05_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6676999.1"
                     /translation="MRSAIRLASVSLVAALWSAVLVPHALDCWAADDAAPRTGPEIYG
                     GVYVFGSLAQNRNLNVGGEALPSTTIKNSAGGGFKAGVFPAFTGYAVGIQAESFGLGH
                     DVKAPASMGASGMQSGRGTLLAWTTMVSLIIQYPGKLIKPYGGVGAGWSSSYLVDAQF
                     MKGAVTQTGTLRDTSPAFQYFAGVRMLVKENVFVFGEYKYFASRYQWSGNLEPSLDFR
                     THIVAVGVGLAF"
     gene            complement(4877..6817)
                     /locus_tag="VFH05_01925"
     CDS             complement(4877..6817)
                     /locus_tag="VFH05_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HET6677000.1"
                     /translation="MPWGSGLIGWNPRLLAFTASLFFLLQVLGVHWASAEADLSYLPE
                     LIEQATAARLADEREWHVLLHYNRNWFGGFTSMQDDPGFFMAPNGKTDPQAELAATLA
                     RFFSDEVVGRSKQPAQCAFIARYYWLNDKLHFDERRLPAHPCERFTRWFNEFNAETIS
                     VIFPTGFMNNPSSMFGHTFLRIDGRGQTSQTRILDYTINFAAEVPPNSGPEYAVKGVF
                     GGYPGHFSTIPYYLKVQEYRDIENRDIWEYRLALTELQVKRLLMHTWELGNAYFDYFF
                     FDENCSYHILSLIEAAEPSIHLLDRFPFYAIPVDTVRVLGQSGIIGEVVSRPSRSTLV
                     RRKREAMTLDERVWLNRMIKDPDELKSDRFRRLALDRQAFILDTASDYLLMKGSGGPE
                     GEPYRQKNRAILSARSHMRVAPNELTIEPYVKRPDLGHGTSRIGVGGGWRNDRAFEEI
                     NVRAAYQDLLDPDPGYTPNAQIEAMSFAVRHYHNESQARLERFSPLNMISLSPIDSLF
                     QAPSWKLGLGMNTIAHRGCQLCTNGYFNTGIGGAVETHLAGRQVFFAMAEVEANASKA
                     YEESHRIGGGGSFGMLADITDRWKILASSTYLRYALGEKSEDWRWSIGQRVSLTTNLA
                     IRVEYNHRNHDDDVLATLHVYF"
     gene            complement(6822..7343)
                     /locus_tag="VFH05_01930"
     CDS             complement(6822..7343)
                     /locus_tag="VFH05_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HET6677001.1"
                     /translation="MMKRVLVLSVAVLFGSQVGIALAAPTGNPDTGPGCGLGKLAWQN
                     YPHQKTIGVQVLEATTNGTFGSGTFGISSGTSGCTNDGQVWASEKANVFAAINFQNLA
                     QDMAQGHGEHLTSLATLMGIPADQQPAFFAMTQERYASFVKAGESSPGAMIKALNDAV
                     ATHPTLAKVSLHK"
     gene            complement(7656..8174)
                     /locus_tag="VFH05_01935"
     CDS             complement(7656..8174)
                     /locus_tag="VFH05_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET6677002.1"
                     /translation="MTRRTLRLGVIADTHGLYDPAVEPHFASVSEILHAGDIGGKDVI
                     HCLRRIAPVIAVSGNVDGYERSGWARRVVLRRGGMTIVLCHVLYENGRMNSEAQAWLD
                     REQPDVCIFGHSHRPTIDRRGRTILFNPGSAGPKRFSLPRGVGILTITGGTAVPSLIQ
                     LEARAWKRAEPR"
     gene            complement(8171..9319)
                     /locus_tag="VFH05_01940"
     CDS             complement(8171..9319)
                     /locus_tag="VFH05_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6677003.1"
                     /translation="MGSATAPTSPNALCFSDGLPSTGLPCHVEISADGLTVRFDENNV
                     LGDVNLPFRALSVSAGGLNHDQLVVRWGTAPSTQTVYLKDPSLIRTFRSAVPPELMKE
                     LDATAAAVRRARSRNRMAWGISAAAIGALMVGIWFSMDLLVGWAVDRIPLDWERRIGE
                     SAYQDFLIQHPAVKDGPAVAAVQEISERLIAHIPDNPYTFEVAVVKSEVVNAFALPGG
                     YVVVFTALLQQAENAEEVAGVLSHELNHVLERHALQRIVKQLGLVTTVGIIIGDQQGL
                     ARVMKQIGVELLSLKFGRSQETEADVTGLQLLHRSHIDPSGMVTFFQRMTEKERGGVE
                     WLSTHPMSAGRAQRLEAEIARLPKTAAEPFTFKWNTVQDALRPVAVRG"
     gene            complement(9321..10004)
                     /locus_tag="VFH05_01945"
     CDS             complement(9321..10004)
                     /locus_tag="VFH05_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET6677004.1"
                     /translation="MKHEILYPGAFPMVRVELAAGETIKAESGAMVASSPTIDIESKM
                     EGGFLGALSRKLLTGEKFFFQTLRASRGAGEVLLAPTVPGEIVIMELDGLNEYMVQKD
                     GFLAGSESIKIESQMQSLSRGLLGGEGFFILKVSGKGSLILNSFGAIHKVELKPDQEY
                     IVDNSHLVAWSGTTSYHIERAASGWVASFTSGEGFVCRFRGPGVVYIQSRNPGSFGAW
                     VRQFIPVSE"
     gene            complement(10030..11499)
                     /locus_tag="VFH05_01950"
     CDS             complement(10030..11499)
                     /locus_tag="VFH05_01950"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6677005.1"
                     /translation="MKPHRTSRLRQGTRSTGPIPTQIVSNEEFTPWPRTPEEALVEEA
                     VVRTSREAAVRLGMTRRHFLKTSGGMAAALLALNSVFGRFFEVFEAELFEPAAFAEQQ
                     GAPYFIFDVQTHYVSTGYDPSDSEARRRGAVTKEALLGLRRRARDTGLNPRLAADRGT
                     IDDLSWQNFVKEVFLDSETSVGLISTPPGPYPQEAVVPPKEMAHIRDELNRLAGSQRM
                     LAHGLATPQLGATDLEFMAQQAEELHIDAWKCYTGSCPKGFDHGWWMDDEKIAYPMLE
                     QARRLHVPRVCVHKGLPLGPVPDYNHPKDLIKAAKDFPDIDFLVYHSGMKSAQSIDEV
                     FAKTGEIPWTTEFCRMKQAHPGVSNIYMELGSTFGQLVTSHPLVCAHLFGQILQAFGD
                     DHVLWGTDSIWYGTPQWQIEAFRRFQIPDPLIEQHHYPSLTREVKAKVFGLNAARLFA
                     VDPAAMRQGIPKDYVGRLRLSYLEEGPEPSHRLYGWVAT"
     gene            complement(11631..>13161)
                     /locus_tag="VFH05_01955"
     CDS             complement(11631..>13161)
                     /locus_tag="VFH05_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677006.1"
                     /translation="VLFARSGPIDEVRPHIPLWSKPAPRLIARLHRIRISIWSFIWSC
                     FSTTRSLTFLALMTGVAVVVVAAAGLAAIGLTWMAMEDAPSLVYQNLTISPPRTITDA
                     KKNGYLMLLGFDAPAGSDALQAGYERKPGPQDKVAAQSCSGGDEAKEDTGPAGASGQV
                     VRGWIRSGDVLSQAKGQSGSIRSFVSRESLTLGRYQQWLAMPFDDWGFGQSLSPNCSR
                     VLLAHRLFLLEGFNQDLFSGIDRLETDMQSWRAALGQSKTLAMKMLALTAVQDDASIA
                     SGLLSRAELDGAALARLNKMVRPLDQAELSVRWPMHSQFVWATKSVSAELKDDPSRDR
                     LWHATVASAMRLPIQRRANAYAEYYEAANKAVAGGRYTNLPKLSSFVRTPPSGVMDYL
                     INPVEHIVGVEPLPSWDPYVMRIVEVDAELRLAGLQAWIRRGPQEGEVLTRLAKAGQA
                     YYDPFTGLPMLVNQRKGLIYSVGRDGKDQEGDSTRDLAVPIPFVSSSDSRRAPLSPPT
                     R"
BASE COUNT         2813 a         3928 c         3753 g         2667 t
ORIGIN      
        1 tcatacggaa cgcctcgaca aaactcctca gagccggata ctctccgagc gaaagtgagc
       61 gtgtcactcc tgtgcgttga ctctcgaatg tgacgcggtc cccttgaatg acataccgct
      121 catgaagcgg atcggtgacg agcttttcca gcgttgccgg tgggatgaac cgcagaatgc
      181 cgcgaacctt caacggttca gtcagcacgg aggaataggt ggcctcttca aatgcgaccg
      241 atggctttcg ctgctccgcg accagggcca tcaacttctc aattggccag tccccgtcga
      301 aagcacggac aatcggaggg aagctcccaa gcgtgagaac caggcagccg agggcacact
      361 ggcgacgcag ttggttcacg atggccatga cgctcttcgc ccacttcggc acgcgtttat
      421 ggcgatgtgc tccatgcctc taggctgagg ggtcaccgac tcaaggtgtg tcctgccaga
      481 agtcatagaa gttaaaccag ttgtcagtgg catttcggct gtagtgcgcg agacggtccg
      541 catactgttg agtccatcgc cgtatgtcgt cctcacgccg ctctgacgag agtcgaatct
      601 cctcgctcag cagttccaat tgcacgtcgt aacgattgtt tccgcggtat aaaccgaaaa
      661 acatgaccac gggcgtgcgg accgcgtgag cgagtcgtat ggatccggtc ggaaagggcg
      721 cgtcggaacc cagaaagttg cagcggacgg tctggttatg ggacatgagt cggtctccca
      781 tgatgccgac gatccctccc ccctccaggc actcccggac gcgcagcatg ttgtcgatgc
      841 ctccgacctg caagacgctg tcggctacga cagggttcat ctcgtgagag aatgcacgca
      901 tcaacggcgc gttttgctcg tccatcagca ctttaatatc gagctcttcg cgcgaaacgc
      961 ccatcgctct caccacttcg aaactgccga gatgcgagcc caggaggagg catccacgcc
     1021 caagcgacag tgcacgagtg agtgtgtcaa ggccattcac tcggatatcg aacagcccga
     1081 aacgcccgcg gagaaaatac actcggtcaa gaatcgtgga cccaaaccag tagtaatggt
     1141 ggaaaagtcc ttgccagcca ggtgaccggc cttgcgcgcg gaggtaatac tggcgcaatg
     1201 tctgattcgc ctcacccgaa cggcagagat aatagagaca gacgggaatg agcagcgccc
     1261 tggccaccgg tcttcccagc gactccgcca cccaagccat caggcggatc aaccgccggt
     1321 ttccgcgttc ctgtcgcgtt ttccaggcaa cggccatcaa ctgcctgatt ggcttccgcc
     1381 cggcaatcga aaccggatgc acccggacgc cacgaggcgg gttcccacct cgcaggtgaa
     1441 ggctgccgtt tcgctctcct gagattcgat ggtaatggtg agccgttctt caggtctgag
     1501 tggagaagaa agtttgaccg ccggcattcc cgtgacgact accccttgcc catggatttc
     1561 tcttagcgct tgaatcactt cctccaggac gatgacgccc gggacgaccg gatgtcccgg
     1621 gaagtgccca ggcagcgccg gatgatcgct gttgatcgtc agcgtccgtt catacgtcat
     1681 gcgacgattc gtccttccac cgctccatga gctgacgcaa cgcttcacgc ggcagcttgc
     1741 ccgtttcgtt gcgaggaagc cgcggcaaaa gcagcaacgg acgcggcaaa aacaccggat
     1801 tcagacgccg cctcagcgca tgctggatgt cttcttgagt cttgccaggc gccacgacca
     1861 atgccatgag acggctcaag gatccttcct tcgcgtcggg caagataaac accccgtcat
     1921 gcaccccttc aacctctaac aactgtcgag tgagatcgcc cagtgatgca cgatgccccg
     1981 caatgttgac ttgatcctcg gcacggccca acacacgaaa tgtcgcccgt ccggtcaccg
     2041 taatgcgatc aggaatcgga acggggcagg gcagatacgg cgcctgtatg gaggcatgag
     2101 agccgttgcg tgtcagggcg acgccgtcga gcatatgcca gtctccgtct gtcaccgtcc
     2161 tccgtgacgc gacgctgcct gcttccgcga acccaaagat ttcatgcacg ggcgcatgaa
     2221 accgctgttc ggcttcgcga gccagagccg aggtgagcgg cgatgtcgcg gagaggatca
     2281 tcccgatggg aggaagcctc acaccggcga gcaaacaggc gcggaggtgc aagggcgtgg
     2341 tgacgagtaa cgatcgctcc ggtgactcgg tcaaggccgc gcggatgtcc tcggggaaca
     2401 gtggccggcc ggcatgcatg gtcagccctt gctgcaccgg cagcatgacg gacgcctcta
     2461 atccaaacat atgctgatga ggcacggtgg cgacgacgcc tttgacgcca gccgcgttca
     2521 cacccagccg cttcgcagtc gctcgtgcca cggacaccaa ggcgtcccat gttttcaggt
     2581 tcggtgtcgg ttgtccggtg ctaccggacg tgaacgcaat ggccacgtgc tgatggagtg
     2641 ggattcgtgg aatgttccag ggcctcatcg agcctctctg ggctggcagc acgacagcag
     2701 gaatgtcgtg gaccggttcc ccccggtccg tcaagcaata gcagctctcc tgctcgacgg
     2761 caatgcggcg cagggtctcg ggcgctcgac tttgcggcag aagcgtcact tgccggcgca
     2821 acatcgcagc cgcaaagccg acaagaaact cgtagcggct cgtgagcaga ttgagcaccg
     2881 acggccggtc cggaagccca tcggccagat tgatcaccgc tgaaagaaac tcacgcgcgg
     2941 agcgagggcc atcggactcc caggccagca cgtcgtccgg gcggtatgag ccgatcagag
     3001 gcgcatctaa catgacacca caggaatcac ttggtgcgat gtaattcgat cgcgcgtgcg
     3061 agcgctcgca gggaggaaaa cgtcgccttg atttcggggt cgctggattt aagttgatac
     3121 ccgtaagttt gcgcgatggc cagcgacagc tctaacgcat cgatggaatc aagccccagt
     3181 ccgtcaccga acagcaccgc ttccggctcg atggaagagg gatccgcctt caacttgagc
     3241 gcctgcacga tcagtttggc gatctcaagt tcaaaaggtg tgagctgttg actgatcata
     3301 gaacggcttc cggtttcacg gcgcggtaat cgcgcaagag cccgttgaac agctcgtgta
     3361 gtgcgcactc gataaatccg gcttctttca aggcagcgac aatggtgtct gctccgacgc
     3421 agtgctcagt cgtatcccac caatactcca tgagcgtctt catgtcgcgg ttccccgttg
     3481 aggctgagaa cgcgatgccc aggaccgtcc tgacgtagaa tcgggagaga gagagcaaaa
     3541 ggggtgaccg cggtcgacaa atatcgagca ggagcacgat gcccccgggc tttaacaccc
     3601 gtcgatactc ggaaaatgcg tgccggagat cggagacatg ccgcaaggca taccccatgc
     3661 tcaagaagtc aaagcaagcg tcgggaaatg gcagccgctc accgatggca ttcaccagcg
     3721 tatgacatgg acctcgcctc gcctcgcgaa gcattccgtt gctaggatcg aggccgataa
     3781 ccgacccgga atgacctacg atttgacggg cacattgcgc aaccagaccg ggcccacacg
     3841 cgacatcgag cacgtgcatg ccggctgtga gtcccgccct cagcagtgct tttcgtcgat
     3901 accagagtcc ggaacccaga ccggttgcct tgtcgatatt gcgatattgg ggggcggtac
     3961 gattgaacag gtcgttgaga aaacgctgac gggcctcatc ttggtcgtag tagcggcgca
     4021 aaagcggatg gggaggcaga ggcacgagct ggccggccga ccgcgtgccg atattatgat
     4081 tggattcggg attcatgaaa cgatttcgcg tccggtccca tcttgtagca ggactctcgc
     4141 agacgggcaa gagggcggtc ggctccatca ttaaaacgcc agaccgactc caacggccac
     4201 gatgtgcgtc ctgaaatcaa gcgagggctc taggtttccg ctccactggt aacggcttgc
     4261 aaaatattta tactctccaa acacaaaaac attctccttc accagcatgc ggacgccggc
     4321 gaaatattga aaagccggac tggtgtcacg gagggtgccg gtctgtgtca ccgccccttt
     4381 cataaactgc gcgtcaacca ggtaggatga cgaccagccc gcgcccacgc ccccataggg
     4441 cttaatgagc ttgccagggt actgaatgat gaggctcacc atggtcgtcc atgccaaaag
     4501 ggtcccacgt cccgattgca tgcccgaagc gcccatggat gcaggagcct tcacgtcatg
     4561 gcccagtccg aacgactcgg cctgaatccc gaccgcatag cccgtgaacg caggaaacac
     4621 gccggccttg aacccgccac cggcggagtt tttgatcgtg gtcgacggga gtgcttcgcc
     4681 accgacattg agatttcgat tttgagctaa cgagccgaag acatagactc ctccatagat
     4741 ttccggtccc gttcttggag cagcatcgtc tgccgcccag caatctagtg cgtggggcac
     4801 taaaaccgcg gaccataaag ccgcgacgag agaaaccgag gccagacgga tggcagatcg
     4861 cacggcaacg gctcggtcag aaatagacgt gcaaggtggc caacacatca tcatcgtgat
     4921 tccgatggtt gtactccaca cggatggcca gattcgtggt cagggagacg cgctgtccga
     4981 ttgaccatcg ccagtcctcg gatttttcac ccagggcata ccgaagatag gtactggagg
     5041 cgaggatctt ccatcgatcc gtgatgtcgg ccaacatgcc gaagctgccg ccaccaccga
     5101 tccggtggga ctcctcatag gctttgctgg cattcgcttc cacttcagcc atggcaaaaa
     5161 acacctgccg cccggcgaga tgggtttcaa ccgctccgcc gatgccggtg ttgaagtacc
     5221 cgttggtgca gagctggcac ccacgatggg cgatcgtgtt cataccaaga cccagcttcc
     5281 aggaaggcgc ttgaaacagg gaatcgatcg gagaaaggga aatcatgttc agcgggctga
     5341 aacgctccag cctcgcttgg gattcattgt gataatgccg cacagcaaac gacatcgctt
     5401 ctatttgcgc gttcggtgta taccccggat cgggatccag cagatcttgg taggcggcac
     5461 gaacgttgat ttcttcaaac gcccggtcat tccgccaacc gcctccgacg ccaatccggc
     5521 tggtcccatg gccgagatcc ggtcgtttga catagggctc aatcgtgagc tcgtttggag
     5581 ccacccgcat gtggcttcga gccgacaaga tggcccggtt tttttgccgg tagggctctc
     5641 cctctggtcc tccgctccct ttcatcaaga gatagtcaga ggccgtatca aggatgaatg
     5701 cttgtcggtc gagcgcgagg cggcgaaagc gatctgactt cagctcgtcg gggtccttga
     5761 tcattcggtt gagccagacc cgttcatcga gggtcatcgc ctcacgtttg cgccggacca
     5821 acgtactcct tgatggccga gaaacgactt cgccgatgat gccagattgg ccgagaaccc
     5881 ggacggtgtc cacgggaatc gcgtaaaacg gaaatcggtc cagcaaatgg atcgagggct
     5941 cggcagcttc aataagcgac aagatgtgat acgagcagtt ttcatcgaaa aagaaataat
     6001 caaaataggc gttgcccagt tcccatgtat gcattaagag gcgctttacc tggagttcgg
     6061 tcaaagccaa gcggtattcc caaatatcgc ggttttcgat atcgcgatat tcctggacct
     6121 tcaggtaata agggatggtc gagaagtgcc cggggtagcc gccaaatacc ccttttacag
     6181 catactcagg tccggagttg ggcggcactt ctgcggcaaa attgatggtg taatccaaga
     6241 tccgagtctg tgaagtttgg cccctgccat cgatccgtaa gaaggtgtgt ccgaacatcg
     6301 aggaaggatt gttcataaac ccggtaggga agatgacgct gatcgtctcg gcattgaact
     6361 cgttaaacca gcgggtaaac cgttcgcagg ggtgtgccgg taatcggcgt tcgtcaaagt
     6421 gcagtttgtc attgagccaa tagtacctcg cgatgaaggc gcattgtgcc ggctgcttcg
     6481 agcggccgac tacttcatcg gaaaaaaatc tggcgagggt cgcagcgagt tcagcctgcg
     6541 gatctgtctt gccgttcggc gccatgaaga accccggatc atcctgcatg ctggtgaatc
     6601 cgccaaacca gtttcggttg taatgaagca ggacatgcca ttcccgttcg tcggcgagac
     6661 gggccgctgt ggcttgttca atcagttcgg ggagataaga gaggtccgcc tcagctgagg
     6721 cccagtgaac cccgagaact tgcagcagaa aaaacaggga cgcggtgaag gcaagtaatc
     6781 gggggttcca gcctatgaga ccggaacccc acggcacgag cttacttgtg caatgaaact
     6841 ttggccagag taggatgggt cgccactgcg tcattcaacg ccttgatcat cgcaccgggt
     6901 gaagattccc ccgccttgac gaaggaggcg tatcgttcct gcgtcatcgc aaagaatgcc
     6961 ggttgctgat cagccggaat gcccatgagc gttgcaagcg aggtgagatg ttccccatgt
     7021 ccttgcgcca tatcctgagc aagattctga aagttgattg cggcaaaaac gttcgccttc
     7081 tctgaagccc agacctgtcc gtcgttggtg cagccggagg taccgctgct gatcccgaag
     7141 gtgccgctgc cgaaggtgcc gttcgtggtg gcttccagca cctggacgcc gatcgtcttt
     7201 tggtgcggat agttctgcca agccagctta ccaagaccac agccgggtcc ggtatcagga
     7261 ttgccggtcg gcgccgccaa cgcaatgccg acttgcgagc cgaatagcac cgctaccgag
     7321 agaaccagca ctctcttcat cataagtcct ccttagtgaa agagttgtcg taccgccggt
     7381 gcctacgatt aataggcagt acctaggccg agtataccca aattcggata tggatgtctc
     7441 acaaaatttc gccggatcgc tgtctgacac agtgtgaggc tcgatattgg gtcggccatg
     7501 cgacgcgcgc gcatccgcgt cgaatgatgg acgctgcacc gacacgtgtc gatcactcgg
     7561 caatcatcga ccatgtcagg cttgcacttc gatcacgcgt gatcagatgg aatgagggcg
     7621 tgcctcgccg atcggtcgca tcacccgacg cattctcatc ttggctctgc acgcttccag
     7681 gccctggctt ccagttgaat caatgacggc accgccgtcc cgcctgtgat cgtcaggatt
     7741 ccgacccctc ggggcaacga aaatcgcttg gggccggcgg aaccgggatt gaagaggatc
     7801 gtgcggccgc gccgatcgat cgttggacgg tgactgtgac cgaagatgca cacatcgggc
     7861 tgttcacgat ccagccaggc ctgcgcctct gagttcatcc gaccgttttc atacagcaca
     7921 tggcacaaca cgatggtcat acctccgcgt cgaagcacga cacggcgtgc ccacccgctt
     7981 ctttcatagc catcaacgtt tcctgatacc gcaatcaccg gcgcaatccg gcgcaagcag
     8041 tggatgacat ccttcccgcc aatgtcgccc gcgtggagga tttcgctcac ggaggcgaag
     8101 tgcggttcta ctgccggatc gtaaaggcca tgcgtgtcgg cgatcactcc aagccgaagg
     8161 gtccgtcgtg tcacccccgg actgcgaccg gcctgagcgc gtcttgaacg gtgttccatt
     8221 tgaaggtgaa tggctccgcc gctgtttttg gcagcctggc aatttccgcc tcgagccgtt
     8281 gagcccgtcc ggcgctcata ggatgggtcg agagccattc cacccctcct ctttcctttt
     8341 cagtcatccg ctgaaaaaac gtgaccatcc ccgaaggatc aatgtgggac cgatggagca
     8401 gctggagccc tgtcacatcg gcttccgttt cctgactacg gccgaatttc aaggagagca
     8461 gctcgactcc aatctgcttc atgaccctcg ccaacccttg ttgatcccct ataataatac
     8521 cgaccgtcgt cactagcccc aattgcttga cgattcgctg gagagcatgt cgttcgagca
     8581 cgtggttcaa ttcgtggctc agcactcctg cgacctcctc ggcgttctcg gcctgttgca
     8641 gaagcgccgt gaacacgacg acatatccgc cgggcagtgc gaaggcgttg acgacctcgc
     8701 ttttcaccac ggcaacctcg aaggtgtagg gattgtcggg aatgtgagca atgagccgtt
     8761 cactgatttc ctgtacagcg gccacagctg gaccgtcttt cacggccggg tgctggatga
     8821 ggaaatcctg atacgccgat tcgccgatcc gtcgctccca gtcgagcgga atccgatcaa
     8881 cggcccaccc gacgagcagg tccatgctga accatatgcc caccatcagg gctccgatgg
     8941 cagccgcgct aatgccccag gccatcctgt tccggcttct cgctcgccgg acggccgcgg
     9001 ctgtcgcatc aagttctttc atcagttccg gtggcaccgc ggaccggaag gttctgatga
     9061 gagacgggtc tttgagatag acggtctgcg tggagggagc cgtcccccat cgcacaacca
     9121 actgatcgtg gttgagaccg ccggccgaca ctgaaagagc gcgaaacggc agattgacgt
     9181 cgcccaaaac gttgttttcg tcaaagcgga ccgtcagtcc gtccgccgat atttccacat
     9241 ggcagggcag cccggtggag ggaagcccgt cgctgaagca cagcgcgttc ggagaagtcg
     9301 gggcggttgc tgaacccaag ttattcgctc actggaataa attggcgcac ccacgcgcca
     9361 aagctaccgg gattacggct ttgtatgtac acgacccccg gcccacgaaa acggcacacg
     9421 aacccttcgc ccgacgtgaa gctcgccacc caccccgaag ccgcgcgctc tatgtgataa
     9481 gaggtggtcc ccgaccaggc aacgagatga ctattatcga cgatgtattc ctgatccggt
     9541 ttcagttcga ccttatggat ggcgccgaag ctgtttaaaa tcaatgaccc cttgccgctg
     9601 actttcaaga tgaaaaatcc ctccccgccc agcagtcctc ggctgaggct ttgcatctgg
     9661 ctttcgatct tgatcgattc ggagccggcg agaaacccgt ctttttgcac catgtattca
     9721 ttgagaccgt ccagttccat gattactatt tcacccggaa cggtcggcgc gagcagcact
     9781 tcccccgcgc cacgactggc ccgcaacgtc tgaaagaaga atttttcgcc ggtgagcagc
     9841 ttccgggaga gcgcgcccag aaaaccacct tccattttgc tttcgatgtc gatcgtcggg
     9901 gaggaggcaa ccatagcacc cgactcggct ttgatcgtct ctccggccgc cagttccacc
     9961 cgcaccatcg ggaaagcccc cggatacaaa atttcgtgct tcatctcttt ccccttatcc
    10021 cgcccgttcc tatgtcgcca cccatccgta caacctgtga ctgggttccg gcccctcctc
    10081 cagataactc agccgaagcc gcccgacgta gtccttcgga atgccctgcc tcatggccgc
    10141 cggatccacg gcgaacagcc gtgcggcatt caatccgaac acctttgcct ttacctctcg
    10201 tgtgagcgac gggtagtgat gctgttcgat caaggggtcc ggaatctgga agcggcgaaa
    10261 cgcttcgatt tgccactgag gtgttccata ccagatcgag tccgttcccc acagcacgtg
    10321 atcatcaccg aatgcttgta agatctggcc gaagaggtgc gcacagacca gtggatgact
    10381 ggtgaccaac tgaccgaagg tcgagccaag ctccatatag atgttgctca cgcccggatg
    10441 cgcttgcttc attcggcaga attcagtcgt ccagggtatc tcgccggtct tggcgaacac
    10501 ctcgtcaatg gattgagcgc tcttcatgcc ggaatggtag acgagaaaat cgatgtccgg
    10561 aaagtctttg gccgccttga tgagatcctt cgggtggttg taatcaggga ccggaccgag
    10621 cggtaatccc ttatgcacgc agacacgcgg cacatgcagc cttctggctt gttccaacat
    10681 ggggtacgcg atcttctcat cgtccatcca ccagccgtga tcgaatcctt tcggacacga
    10741 gccggtgtag catttccagg catcgatgtg cagttcctcc gcttgctgcg ccatgaactc
    10801 caaatccgtc gcacccaatt gcggtgtggc caacccgtgc gcgagcatcc gttgggaacc
    10861 ggcgaggcgg ttgagctcat ccctgatatg cgccatttcc tttggcggca cgaccgcttc
    10921 ttgtggatag ggtcctggag gagtgctgat cagcccgacc gaggtttcgc tgtcaaggaa
    10981 cacctccttg acgaagtttt gccacgagag atcatcgatc gtgccacggt cagccgcgag
    11041 tcgtggattg agtcccgtgt cacgcgctct ccgcctcaag cccagcaaag cctcttttgt
    11101 taccgccccc cgacggcgcg cttccgaatc agacggatca tagccggtgc tgacataatg
    11161 ggtctggacg tcgaagatga agtagggagc gccctgttgc tcggcgaaag ctgcgggctc
    11221 gaacagttcg gcttcgaaga cttcgaagaa acggccaaac accgaattca gggcgagcaa
    11281 tgccgccgcc atcccgcccg aggtcttgag gaaatgccgg cgagtcatac cgaggcgcac
    11341 ggccgcttcg cggctcgtcc tgaccacggc ctcctcgact aacgcttctt ccggcgtgcg
    11401 tggccatggc gtgaactcct cgttcgatac gatttgggtg ggaatcggtc cggttgaacg
    11461 cgtgccttgc cggaggcgcg aggtccgatg tggtttcatg gtatccctcc ggaggtggaa
    11521 cggctccggt ggattttgtg gaataccccg gaccctgtca agcatacatc cgcctgcccg
    11581 acgcaggaaa cggacgcacc ggcgtgaacc gggctgcaga catgccgtgt ttatcgggtt
    11641 ggaggagaaa gtggagcccg tctggagtct gaagatgaaa cgaagggaat agggacggcc
    11701 aggtcacgag tgctgtcccc ttcttggtcc tttccgtcgc gtccgacgct gtagatgagc
    11761 cctttccgct gattgaccag catgggcagg ccggtaaaag ggtcgtagta ggcttggccg
    11821 gctttggcca accgcgtgag aacctcgcct tcctggggac ctctccggat ccaggcctgg
    11881 agtccagcca aacgcagttc ggcatcgacc tcgacgatcc gcatgacgta cgggtcccat
    11941 gaaggaagtg gctcgactcc gacaatgtgt tcaaccggat tgatgagata atccatcacc
    12001 ccggaaggag gcgtccgcac aaaggacgag agcttcggaa ggttcgtgta acggcctccg
    12061 gccaccgcct tgttcgccgc ttcataatat tcggcgtagg cattcgctcg gcgttgaatg
    12121 ggcaaccgca ttgccgacgc cacggtcgca tgccataggc ggtctcgact cggatcgtct
    12181 ttgagttcgg ctgaaactga tttggtggcc cacacaaatt ggctgtgcat cggccagcgt
    12241 acggagagtt cagcctggtc gagagggcgc accatcttat tcagccgcgc aagggctgct
    12301 ccgtccaact ctgcgcggct cagcaatccc gaggcgatcg aggcatcatc ttgcaccgcc
    12361 gtgagggcaa gcatcttcat ggccaaggtt ttggattgtc ccagcgcggc tcgccacgac
    12421 tgcatgtcgg tttccagtcg atcgattccg ctgaaaagat cttgattgaa tccttccagc
    12481 aggaagaggc ggtgcgccaa caagacccgt gaacaattgg gactcagcga ttgtccaaat
    12541 ccccaatcat caaacggcat ggccagccac tgctggtaac gaccaagcgt gagggattcg
    12601 cgcgagacga acgagcgaat actcccgctc tgtcctttgg cttgagaaag aacgtcacca
    12661 ctgcggatcc atcctctgac cacctgccct gaagcgccgg ctggtcctgt gtcttctttc
    12721 gcctcgtcgc ctcccgagca actctgtgcc gcgaccttgt cctgcgggcc cggtttgcgc
    12781 tcgtacccgg cttgcagagc gtccgatcct gctggggcgt caaatcccaa caacataaga
    12841 tagccgttct ttttcgcatc cgtgatcgtt cgtggtggac taatggtcaa attctgatac
    12901 accaaactcg gcgcatcttc catggccatc catgtcaacc cgatcgcagc aaggcctgcc
    12961 gcggctacca ccacgaccgc cactccagtc atcagggcca gaaaggtgag agaccgggtt
    13021 gtggaaaagc acgaccagat gaacgaccag atgctgatcc ggatccgatg caggcgagcg
    13081 atcaaacggg gagccggttt cgaccacaaa ggaatatgcg gccggacctc atcgatgggt
    13141 ccgctccgtg cgaacaacac a
//
LOCUS       DASSCQ010000027        12377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1262292, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000027 DASSCQ010000000
VERSION     DASSCQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12377
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262292"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VFH05_01960"
     CDS             <1..1347
                     /locus_tag="VFH05_01960"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET6677007.1"
                     /translation="QPSLPDGTAVGDITSHSAVLWLKTAGSKHVQVEWASVEAWKTMS
                     AMASVRSAAARTPVLVTSVDTDFTLSILIDGLNPGTRYRYYIVVVGPESPSKRHVAAE
                     GEFGTLPDPATSAAVTFAWSGDLGGQSQCRRGPEGYPIFDTIRKQRPDFFIFLGDTIY
                     ADHLCASPPNEPGANFVAMTLSEYRARHHYQRGAAALRRMLQTVPVYVMWDDHEVKNN
                     FSGPFEPQMPAGRQALRDYWPIQIDASDPHRLYRRVRYGSDLELFLLDTRQYRSRNAD
                     PDGPAKTMLGGPQLTWLLDGIAGSNATWKVIVTSVPLSILKGGGLTVPGYDGWAGGAE
                     ANGFERERQLIVDAILQKKLKNVVFLAGDIHWVQANVYDPNEDGVADFHEFAVGPLSA
                     RPGWLSAPSATLHPTTLINEAGYANFGLVRVTKEAFALSVIDEAGVTRFSYRIAAR"
     gene            1534..1779
                     /locus_tag="VFH05_01965"
     CDS             1534..1779
                     /locus_tag="VFH05_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677008.1"
                     /translation="MLLQLMSVVGALMVLAAYGLNQGGVWRELHAGYLTLNIVGSLML
                     GVVAIADQRAGFILLEFAWAGLGLVGVARVIRSRRQP"
     gene            1841..2329
                     /locus_tag="VFH05_01970"
     CDS             1841..2329
                     /locus_tag="VFH05_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677009.1"
                     /translation="MAGLMSAEEVFEKTQAAAVAATAPDEKALQIDYPALRDKIRAAL
                     ADRKVALCHVNKFLPEGYEEQGRFNVLLLTAGNVFFDMVIGDSYFRYDVVSVAQLDKV
                     QVIDAMWDNKEKRREEPFLSLRLMHGEEAHLLLALDEEERVSLLAFAKAVAAARNPER
                     AS"
     gene            complement(2326..3450)
                     /locus_tag="VFH05_01975"
     CDS             complement(2326..3450)
                     /locus_tag="VFH05_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HET6677010.1"
                     /translation="MTTNTAMKHSRLHDHLAKLGATFEEVTGWLTPVHYGQWVDEYRA
                     VREGVGIADLSHRGKLRVTGDDRVKWLQSVISNDILPLQPGQGRYSSLLTHKGKMLTY
                     FRCYMQTDAVVLEDAGEIGDQTYTALRKFLLYGTKAKLENCRESWGLLLVSGPKAAMV
                     VKAAFGTDLADLKPVNFISAAIGGQTALMIRAEETGEEDIEILIPVDGLIAAWERLME
                     AGAAHGIRAVGSQAREALRMEAGIAKAGPDLNEEIVPPEANLEEKAFSLSKGCYPGQE
                     VVARMDTYGTVRRHLVGLVVKGSVVPPKGAKLFTGDREVGWISSASLSPELQAPIAFG
                     FPLRDFSAAGTALSVHAEGGRYEATVQTLPFHRPKGPHAG"
     gene            complement(3468..3857)
                     /locus_tag="VFH05_01980"
     CDS             complement(3468..3857)
                     /locus_tag="VFH05_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6677011.1"
                     /translation="MTHIHSKTILVAVTDIFFYTKVRDALLPNGYTLERARSQEDIAA
                     KASTAQPAAVILNMNDETLNAFQVLETLKADPRFHTLPILAFANHEEVETWNRAKSMG
                     VTKIVSRNEFSMRTRDLVEGVISHIAS"
     gene            complement(3892..4407)
                     /locus_tag="VFH05_01985"
     CDS             complement(3892..4407)
                     /locus_tag="VFH05_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677012.1"
                     /translation="MDVQDFLLQGEGSEEDKREAWELFQRAYQRQMKGELEEAVSLYR
                     QSIATHPTAEAHTFLGWTYSFMGRLDDAIEECHRAIARDPEFGNPYNDIGAYLIEKGE
                     LDDAIPWFQKAMHAKRYESPAFPHLNLGRVYEKKGNWTEAISSYKQALALNPDYVLAK
                     KSLGRLVSMLN"
     gene            complement(4553..5353)
                     /locus_tag="VFH05_01990"
     CDS             complement(4553..5353)
                     /locus_tag="VFH05_01990"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HET6677013.1"
                     /translation="MGKPKPAPLRERDITRQIAREYYKEFDQLIESDVIIVGAGPSGL
                     ICAHDLADMGFRTVIIEQSLALGGGFWHGGYLMNKATICAPAHKILEEIGVPCKKVKD
                     CEGMYIVDPPHATASLIAAAYRAGARVLNLTRVVDLILRREGILEGVVVNNTTAEMAG
                     HDIIHIDPIALESKLVVDATGHDAVIVNLLHKRNLYTEVPGNGAMWVSRSEQDVMDHT
                     GEVYPNCFVIGLAVAAVYGTPRMGPAFGSMLLSGRYGAELIKKKLKQE"
     gene            complement(5407..7290)
                     /gene="thiC"
                     /locus_tag="VFH05_01995"
     CDS             complement(5407..7290)
                     /gene="thiC"
                     /locus_tag="VFH05_01995"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET6677014.1"
                     /translation="MTTNGHPTGTPPVTTQPFPASRKVFVTGTRPGVRVPMREIALTP
                     TKSPKGGAPTENEPLVVYDTSGPYTDQSVTIDLRRGLAPLRQDWILGRGDVEQLSDVT
                     SEYGRLRAADPKLTDLRFQHIRKPLRAKRGLNVTQLHYARRGMITPEMEFIAIRENQS
                     REVAREVASRNGVGGGVAQHPGQAWGASIPKVITPEFVRDEVARGRAIIPANINHPET
                     EPMIIGRNFLVKINSNIGNSAVSSTIEEEVEKMIWSTRWGADTVMDLSTGKNIHETRE
                     WIIRNSPVPIGTVPIYQALEKVGGKAEDLTWDIFRDTLIEQAEQGVDYFTIHAGVRLA
                     YVPLTANRMTGIVSRGGSIHAKWCLAHHQENFAYTHFEEICDIMKAYDVSFSLGDGLR
                     PGSIADANDESQFAELETLGELTKIAWSHDVQVMIEGPGHVPMHLVQVNMEKQLKECH
                     EAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHLGLPDREDVKTG
                     VITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLSLDPDTAKDFHDETLP
                     DQAAKVSHFCSMCGPHFCSMKITQDVREYAASKRVTEQQAIQIGMKEKAEEFKQSGSE
                     IYR"
     regulatory      complement(7376..7478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7641..8744
                     /gene="nadA"
                     /locus_tag="VFH05_02000"
     CDS             7641..8744
                     /gene="nadA"
                     /locus_tag="VFH05_02000"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET6677015.1"
                     /translation="MNSVATLPRAVTDYQLLTAEELRQRIAEAKRTLGDQVMVLGHNY
                     QRDDVIEHADFRGDSLLLSKLAAERSERPHVVFCGVHFMAETADILSRTNQTVILPDM
                     AAGCSMADMAAIEQVDQCWETLGRLLPVEETVMPAVYVNSAAVLKAFCGEHGGITCTS
                     SNAKAVIEWSWARREKILFFPDEHLGRNTANKMGIAREHMIVWDPFQPNGGNTRDAIR
                     RAKLILWKGHCSVHQMFQAAHVDNFRKQYPQGKVIVHPECHEDVVNKADLAGSTEFII
                     KTVTAAPAGTVWAVGTELNLVNRLKREMTDKKVFFLSSTVCQCATMFRIDAAHLCWAM
                     ENLADGHIVNHIVVPEDDKRWARIALDRMMSVS"
     gene            8987..11785
                     /gene="ileS"
                     /locus_tag="VFH05_02005"
     CDS             8987..11785
                     /gene="ileS"
                     /locus_tag="VFH05_02005"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET6677016.1"
                     /translation="MDYKSTLNLPKTDFPMKANLPQREPELLAWWEQERLYTRIQEAG
                     KGRPRYLLHDGPPYANGRIHIGHALNKILKDIIVKSKTMAGFHAPYVPGWDCHGLPIE
                     HQVLKDLGEKKHALDAMGIRQLCREYAEKFYKIQREEFQRLGVLGEWQHPYLTMNPAY
                     EATIVREFGKFVERGGVYKGLKPVLWCTQDQTALAEAEVEYEDHVSPSIYVKFPIAGG
                     AAAIAAKLPGVNLPSDVTTVSVVIWTTTPWTLPANQAVCLHQDIDYAFVRVGSEVVIV
                     AEQLVDGMAKTCKLEGYRVLAVRKGGEGFEGLETQRPLTTGLSPVLLGDFVTLEQGTG
                     CVHIAPGHGMEDYILVLEHNAKASPGERLEILAPVDDAGRFTQAVPEFAGQHVFKANP
                     GIVELLRTKGRLIGHGSLAHSYPHCWRCRSPVIFRATEQWFVSMDINALRQEALAEID
                     RVKWIPPWGKDRINGMIANRPDWCLSRQRVWGVPIPAFTCTGCRRVLADPRIIDHIGS
                     IMEEHGADVWFKRPAAELLPAGTICPHCNGNQFEKERDILDVWFESGVSFAAVLKPCR
                     WWPADLYLEGSDQHRGWFHSALLAGIVTDHRAPYEAVLTHGFVLDGQGKKMSKSAGNV
                     VAPQDVITQSGAEILRLWVSAQDYRDDVRISSEILNHLIEAYRKIRNTCRFLLSNLYD
                     FDPATHRVPYDQLPELDRWALMRLEEIKTKVRRAYDEFEFHAIYHALNNYCSVDLSAV
                     YLDILKDRLYTFRAGDPLRRGSQTVLYEIIVAVTKLMAPILSFTAEEIWLSLGVQRGG
                     HSSVHLGQFPEASPGWHDAKLAQRWERLLAYRTQVQGVLEVSRRDKVIGSSLEAAIEI
                     RADADALAFLKTYERDLPTLFIVSHVRLTAISGGPATVVATKSSFAKCERCWNYREAV
                     GKDAAHPTLCDRCVEALR"
     gene            11782..12309
                     /gene="lspA"
                     /locus_tag="VFH05_02010"
     CDS             11782..12309
                     /gene="lspA"
                     /locus_tag="VFH05_02010"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET6677017.1"
                     /translation="MTRSSRYLVLALIGVVVIVLDQITKYWVMQSMRLHESIPIVPNL
                     FSFTYIRNPGAAFGLLAGSSNAFRTVFFGITSLFALALLGTILLRLPEKDWIGQLSIA
                     GILGGAIGNLIDRLRFGEVIDFLDVYVESYHWPAFNVADSAISVGVVCLIIHFAFERK
                     EAPLPAPDTPPPTGC"
BASE COUNT         2441 a         3538 c         3609 g         2789 t
ORIGIN      
        1 cagccgagct tgccagacgg cacggcagtc ggagacatta cgagccactc tgccgtgctc
       61 tggctgaaga cagccggctc aaagcatgtc caagtggaat gggcctccgt cgaggcctgg
      121 aaaacgatgt cggcgatggc gtcggtgcga tcagccgctg cccgaacgcc ggtgttggtg
      181 accagcgtcg acacggattt taccttgtcc atcctcatcg acggcctcaa tccagggact
      241 cggtaccgct attacattgt ggtggtgggt ccggagagtc cgtccaagag gcatgtcgct
      301 gctgagggcg agttcggcac gctgcccgat cctgcaacct cagcagctgt caccttcgcc
      361 tggagcggcg atttgggcgg acaatcccaa tgccgccgag ggcctgaggg ctatccgatt
      421 ttcgacacga tccgcaaaca gaggccggat ttcttcatct ttctcggtga cacgatctat
      481 gcggaccatc tatgtgcctc tccacccaat gagccgggcg caaacttcgt cgccatgacc
      541 ttgtccgagt atcgtgccag acaccattac caacgcggcg cggccgcact ccgccggatg
      601 ctccagacgg tccctgtcta tgtgatgtgg gacgatcatg aggtaaaaaa taatttctca
      661 ggaccgttcg aaccgcaaat gccggctggc cggcaggcac tgagggacta ctggccgatc
      721 cagatcgacg caagcgatcc acaccgcttg tatcgccgcg tccggtacgg gtctgacctc
      781 gagttgtttc ttctggatac caggcagtac cgcagtcgca atgcggatcc agacggtccg
      841 gccaaaacga tgttgggcgg tccacagctc acatggctgc tggatgggat agctggttcg
      901 aacgcgacct ggaaagtcat tgtcacgagt gtgccattgt ccatcctgaa gggaggcggg
      961 ctgaccgtgc cggggtatga cggatgggct ggcggggcgg aagccaacgg attcgagcgg
     1021 gaacgccaac tgattgtcga tgccattcta cagaaaaagc tcaagaatgt ggtgtttctc
     1081 gccggcgaca tccactgggt ccaggccaat gtctatgacc ccaatgaaga cggagtcgca
     1141 gacttccatg aatttgcggt tggtcccttg tctgcacgac caggctggct gagcgcgccg
     1201 agcgcgacgc tgcatccaac gaccctcatc aatgaagcag ggtatgcgaa cttcggcttg
     1261 gtccgagtga cgaaggaggc atttgccttg tcggtgattg atgaggccgg tgtcacacga
     1321 ttttcctacc gcatcgcagc tcgctaacat ccgccttctt atcgccactt gcattcctgc
     1381 tcgttcaccg gttaccgtac cagctttgtt ggatcgcaaa agccccgccc ggcttgctct
     1441 tggatggttc cacgcgcatt gcaccgttgc tctggtaccg ctgtccttcg ggcgttgtgg
     1501 acggagttac tccctgtttg agaggaatgt tgaatgctct tacagctgat gtccgtcgta
     1561 ggggcgttga tggtcttggc ggcctatggg cttaatcagg ggggggtctg gcgcgaactg
     1621 catgccggtt acctgacact caacatcgtc ggttctctga tgctgggtgt ggtggccatt
     1681 gcggaccagc gggccggatt cattttgttg gagttcgcgt gggccggtct cggcctagtc
     1741 ggagtcgcga gggtcatccg ttcaagacgg cagccctagc agcagaagcg gatgagcaat
     1801 gactcggcat tttttccctt agagagaagg agagaacgtc atggctgggt tgatgtcagc
     1861 ggaagaagta ttcgaaaaga cacaggccgc ggcagtcgcc gcgacggctc cggatgaaaa
     1921 agccttgcag atcgattacc cggccttacg cgataagatt cgcgcggccc ttgccgatcg
     1981 caaggtagcg ctctgccacg tcaataaatt tctgccggag ggctacgaag aacaggggcg
     2041 gttcaacgtc ctgctcctta cggcgggcaa cgtgtttttc gacatggtca tcggcgattc
     2101 ctatttccgc tacgatgtgg tttcggttgc tcaattggac aaggtgcagg tgatcgatgc
     2161 catgtgggac aacaaggaga aacggagaga ggaacctttt ctcagcctgc ggctcatgca
     2221 cggcgaagag gcacatttgt tgctcgcgct ggatgaggaa gagcgtgtca gcctcttggc
     2281 ctttgcgaag gccgtggccg ccgcgcggaa tcctgagcga gcctcttagc cggcgtgagg
     2341 acctttgggg cgatgaaagg ggagggtttg aaccgtggcc tcatagcgtc cgccttctgc
     2401 gtggaccgat agcgccgtgc ccgcagcgct gaaatcgcgc aacggaaatc cgaatgcgat
     2461 cggagcctgt agctcaggcg acagactcgc gctgctgatc cagccgactt ctcggtctcc
     2521 ggtgaagagc ttcgcgccct ttggcggcac gacggagccc ttgacgacta aaccgactag
     2581 gtgccgtcgc acggtaccgt aggtatccat ccgcgcgacc acttcctgac ccggatagca
     2641 ccctttactc aagctgaacg ctttctcctc gaggttggct tcaggcggaa cgatttcttc
     2701 gttcaggtcg ggaccggcct tcgcaatgcc cgcttccatg cggagcgcct cgcgcgcttg
     2761 actaccgacg gccctgatcc catgcgccgc tcctgcttcc atgagccgct cccaggcggc
     2821 gatcaaaccg tcgacgggaa tgagaatttc gatatcctct tcgcctgtct cctccgcacg
     2881 gatcatgagc gccgtttgcc cgccgatggc ggcggaaatg aagttcacag gtttcaggtc
     2941 ggcaagatcg gttccaaacg ccgccttcac caccatcgcc gcctttgggc cgctgaccaa
     3001 cagcagaccc cagctctctc ggcagttctc gagtttggct tttgttccgt acagcaggaa
     3061 tttgcgtaat gctgtatagg tctgatcacc gatctccccg gcatcctcca ataccactgc
     3121 gtcggtctgc atgtagcagc ggaagtacgt cagcattttc cctttatggg tgaggaggct
     3181 ggaatagcgg ccttgccccg gctgcagcgg aagtatgtcg ttgctgatca cgctttggag
     3241 ccatttgacc cgatcgtcgc cggtgacgcg aagtttgcct cgatgtgaaa gatcggcgat
     3301 acccacgccc tctcggaccg cccggtactc gtccacccat tggccatagt gaaccggcgt
     3361 caaccacccg gtgacttctt cgaacgtggc gccgagtttg gcaagatggt catgcaagcg
     3421 ggagtgtttc atggcggtat tggtggtcat gtgacgtcct cgtggcgtta cgaggcaatg
     3481 tgggagatga ccccttccac gaggtccctc gtccgcatgg aaaattcatt tcgtgacaca
     3541 atcttcgtca cgcccatgga cttagcccga ttccacgttt ccacctcttc atggttcgca
     3601 aaggccaaga tcggaagcgt gtggaatcgc ggatcggcct tcagcgtctc caagacctga
     3661 aacgcgttca acgtttcgtc attcatgttg agaatcacgg cagctggttg tgccgtcgaa
     3721 gccttggctg caatgtcctc ttgggatcgt gctctctcca gggtgtaccc gttcggcaac
     3781 agggcatccc ggactttcgt gtagaaaaaa atgtccgtga cggcgaccag tatggttttc
     3841 gaatggatgt gagtcatggt ctgcatgcgg ctaaaacggt aagcgcgatc atcaattcaa
     3901 catgctcacc agtcgcccca gcgacttctt cgccagcaca taatcgggat ttaatgccaa
     3961 tgcctgtttg tacgacgaga tggcttccgt ccagttaccc ttcttctcgt acacgcgccc
     4021 cagatttaaa tgggggaacg ccggactctc gtaccgtttc gcatgcatgg ctttttgaaa
     4081 ccatgggatg gcgtcgtcca gttcgccctt ctcgatcaaa taggcgccga tgtcgttgta
     4141 gggattgccg aactcgggat cccgggcaat ggcgcgatga cactcctcga tcgcgtcgtc
     4201 gagccggccc atgaaactat aggtccagcc gaggaacgtg tgagcttcgg ccgtcggatg
     4261 ggttgcgatg gactgtcggt agagcgagac ggcttcttcc aattcgcctt tcatctgccg
     4321 ttgataggcg cgttgaaaga gctcccacgc ttcacgcttg tcttcctcgc tgccttcgcc
     4381 ttgtagtaag aaatcctgca catccatgat acggctcttg aaacccgcca ccagcatcgt
     4441 tctcagtcgc tctcccccct cgacgtacga ttaagagtac gcctcggggg tctctctccc
     4501 tgcggcttgc tgagtgacgg gtttgaagag ccgcgggaat agtggttctc cgctactcct
     4561 gcttcaattt cttcttgatc aattctgcgc cgtaacgacc cgacaataac atcgaaccga
     4621 aggcagggcc catgcgtggt gtcccataca cggccgccac ggccaatcca atgacgaaac
     4681 agtttgggta cacttccccg gtatggtcca tcacatcctg ctctgaacgt gagacccaca
     4741 tggccccgtt gccgggtact tccgtatata gatttcgctt gtgcaaaagg ttcacgatga
     4801 ccgcgtcgtg accggtcgca tcgaccacaa gtttgctctc cagcgcaatg ggatcgatat
     4861 gaatgatatc gtggccggcc atttcggcgg tcgtgttatt gaccacgacg ccttcgagta
     4921 taccctcccg acgaaggatc aagtcgacga ccctcgtgag attgagcacc ctggcaccgg
     4981 cccggtaagc tgcagcgatc aagctcgccg tggcatgcgg cggatcgacg atatacatgc
     5041 cttcgcagtc tttgaccttc ttgcatggca caccgatttc ctcgaggatc ttatgcgccg
     5101 gagcacagat cgtcgccttg ttcatcagat agccgccgtg ccagaacccg ccgcccagcg
     5161 caaggctctg ctcgatgatg acggtgcgga agcccatgtc cgccagatca tgcgcgcaaa
     5221 tgagacccga aggaccggca ccgacgatga tgacatcgct ttcgatcaac tggtcgaatt
     5281 ctttgtagta ctcgcgcgcg atctgcctag tgatgtcgcg ttctctcagc ggcgcgggct
     5341 tgggttttcc catggcagta ctcctcggtg gatgccggta cgagtgggca gccacccgat
     5401 gcagagctaa cggtaaattt ctgaaccgga ttgtttgaac tcctccgcct tttccttcat
     5461 gccgatctgg atcgcttgtt gctcggtcac ccgtttgctg gccgcatatt cacgaacgtc
     5521 ttgcgtgatc ttcatggagc agaagtgtgg cccgcacata ctgcaaaaat gcgagacctt
     5581 ggctgcctgg tcgggcagcg tctcatcatg gaaatccttc gccgtgtcgg gatcgagcga
     5641 caaattgaat tgatcttccc aacggaattc gaagcgggcc ttcgagagag cattgtcccg
     5701 gatctgcgct ccgggatgcc cttttgccag atcggccgca tgcgcggcga tcttgtaggt
     5761 gatgacgccg gtcttcacat cttccctatc cggcaagccg agatgttctt tgggtgtcac
     5821 ataacagagc atcgcgcatc cataccagcc gatcatcgcg gcgccaatcc cggacgtaat
     5881 atggtcgtac ccgggggcaa tgtcagtggt gagcggtccc agggtgtaga acggtgcttc
     5941 gtggcattct ttgagctgtt tttccatatt gacctggacc agatgcatcg gcacgtggcc
     6001 gggcccttcg atcatgacct gcacgtcgtg cgaccatgcg atctttgtca gttcgcccaa
     6061 cgtttccagt tcggcgaact ggctctcatc gttcgcgtct gcgatcgagc ccggccgtaa
     6121 gccgtctccg aggctgaacg acacgtcgta ggccttcatg atgtcgcaga tttcttcaaa
     6181 atgcgtgtag gcgaagtttt cttggtgatg agccagacac cacttggcat ggatcgaacc
     6241 gcctcgggac acgatgccgg tcatacgatt ggcggtcagc ggcacgtagg cgagccggac
     6301 ccccgcgtgg atggtgaagt aatcgacccc ctgctccgcc tgctcgatca acgtgtcacg
     6361 gaaaatgtcc cacgtgaggt cttcggcttt gccgccgact ttctcaagcg cctgatagat
     6421 gggaaccgtc ccgatcggga cgggggagtt gcggataatc cactctctgg tttcatggat
     6481 atttttcccg gtcgagaggt ccatgacggt atccgcgccc caccgcgtcg accagatcat
     6541 cttttcgact tcttcctcaa tggtcgagct gacggcggaa ttgccgatat tcgaattgat
     6601 cttcaccaaa aaattgcgcc cgatgatcat cggctctgtt tccggatgat tgatattggc
     6661 gggaatgatc gcacgccccc tcgcaacctc atctctcacg aattccggcg tgatcacctt
     6721 cgggatagag gcgccccacg cctggccagg atgctgggcg acgcctccgc caacaccgtt
     6781 gcgcgacgcc acttcacggg ccacctcgcg cgactgattt tcgcggattg cgatgaattc
     6841 catctccggc gtgatcattc cacggcgggc gtagtgcagc tgggtgacgt tcagtcctcg
     6901 tttggcgcga agcggtttgc ggatatgctg gaatcggaga tcggtcagct tggggtcggc
     6961 ggcacggagg cggccatact ccgatgtcac gtcagacagc tgttcaacgt cgccacgacc
     7021 gagaatccaa tcttgtcgaa ggggggcaag ccccctgcgc aggtcgatcg tcaccgactg
     7081 atcggtgtag ggcccggagg tatcatagac gacgagcggc tcgttctcag taggtgcccc
     7141 gcccttcggt gatttggtgg gcgtcaaggc gatctctcgc atcggcacac gcacccctgg
     7201 ccgggtgccg gtgacgaata cttttcgcga ggccggaaac ggctgggtgg tgacgggagg
     7261 agtgccggtc ggatggccgt tagtcgtcat ggaagatcct ttcatcggat cgtgaacgat
     7321 cgaggtgggg caataaaaaa gccgcacctt gcaggaggag agtgcggctg agacggtcgt
     7381 cagtgatccg cttccctacg ctggtattac ccaggtcagg ttcgaagggt catccgatcg
     7441 ctcggattct cagccgtgtg gcccccccag ctctgggttg aaatcgtatc gccgcctcct
     7501 tatgaagtca acgggttaat agccctatgc cgctgctccc gaatggtccc cgacaaggtc
     7561 ttcttcccaa ccctgtttca ttgattggcc ggtaatgcat atcgtagaat gacgcgctag
     7621 atgatattgc tgaggacctt gtgaacagcg ttgcgacctt acccagagcg gtgaccgatt
     7681 accagctcct gaccgcagag gaactgcgcc agcgcattgc ggaggcgaag cgcacgctcg
     7741 gagaccaggt gatggtgctc ggccataact atcaacggga cgacgtcatc gaacatgcgg
     7801 atttccgtgg cgattcgctg ctcctttcca agttggcggc tgaacggtcg gagcgacctc
     7861 atgtagtctt ttgcggcgtg cacttcatgg cggaaaccgc cgacatcctg agccggacca
     7921 atcagacggt tattcttccc gacatggcgg ccggttgttc catggccgac atggccgcga
     7981 ttgaacaggt ggatcagtgt tgggagacgc tgggtcgtct cctacctgtc gaggagaccg
     8041 tgatgccggc tgtgtatgtc aattcggccg cagtcctcaa ggcattctgc ggcgagcacg
     8101 gcggcatcac ctgcacctcc tcgaatgcca aggcggtgat tgaatggtcc tgggcgcgcc
     8161 gtgaaaagat tctgtttttc cccgacgaac acttgggacg caatactgcc aacaaaatgg
     8221 gcatcgctcg agagcacatg atcgtctggg atccgtttca accgaatggc ggcaatacca
     8281 gggacgctat ccggcgggcg aaactcattc tatggaaggg tcattgcagc gtgcaccaaa
     8341 tgtttcaagc cgctcacgtg gacaactttc ggaaacagta tcctcagggt aaagtgattg
     8401 tacatcccga atgtcatgaa gatgtcgtca ataaggccga tctcgccggc tccaccgagt
     8461 tcatcatcaa gacggtcacc gccgcgccgg ccgggacagt gtgggcggtc ggaaccgagc
     8521 tgaacttagt gaatcgtttg aagcgtgaga tgaccgataa gaaagtgttt tttctgtcct
     8581 ccactgtgtg tcagtgtgcg acgatgtttc ggattgatgc cgcacacctg tgctgggcga
     8641 tggaaaatct cgccgacggg cacattgtga atcatattgt cgtgcctgag gacgacaagc
     8701 gttgggcgag gatcgccctt gatcgcatga tgtccgtcag ctaggggcat tgtgaggaac
     8761 gccctcttct cagcgcctac ccacggctcg ccgagacgtg cccttcacca cacttcgttc
     8821 tcgcatcgct ccaaccttca cgcacatgaa tcgcacgctt caggcgctca ctcgccgcgg
     8881 ccttgcggca agtcgttttt gacgatccgg caggaagtct aagggctagt acctcgctcg
     8941 acgttccggt atattcactt catttccgcc caaaccgtct gtacctatgg actacaagtc
     9001 gactctcaat ctgccgaaaa ccgattttcc gatgaaggcc aatctgcctc agcgggagcc
     9061 ggaattgctc gcatggtggg agcaggaacg cctgtatacg cgcattcaag aagccggcaa
     9121 gggccgcccc cgctatctcc tccacgatgg acctccttac gcgaacggcc gaatccatat
     9181 cggccatgcg ctcaacaaga tcttgaagga catcatcgtc aaatcgaaaa caatggcagg
     9241 cttccacgcc ccctatgtcc cggggtggga ctgccatggc cttccgatcg agcaccaggt
     9301 gctcaaagat ctgggcgaga agaagcacgc gttggacgcg atggggattc gccaactttg
     9361 ccgggaatac gccgagaaat tttacaagat ccagcgagag gagtttcagc gattgggcgt
     9421 tctcggcgag tggcagcatc cgtacctgac gatgaatcct gcgtacgagg cgacgattgt
     9481 ccgcgagttt ggcaaattcg tggagcgggg cggtgtctat aaaggcttga agcccgttct
     9541 ctggtgtact caggatcaaa ccgcgctggc ggaggccgaa gtcgaatatg aagatcacgt
     9601 gtcgccgtcg atctatgtca aattccccat agcagggggg gccgctgcaa tcgccgcgaa
     9661 gctgcccgga gtcaatctgc cttcagatgt caccaccgtg tcagtggtca tctggaccac
     9721 gactccttgg accttgccgg ccaatcaagc ggtctgcctg catcaggaca tcgactatgc
     9781 cttcgtgcgc gttggatcgg aagtcgtcat cgttgcggaa cagctggtcg acggcatggc
     9841 gaagacctgc aagcttgagg gatatcgtgt gctcgccgtg aggaaaggcg gggagggatt
     9901 cgaagggctt gaaacccagc gaccgctgac caccggcctc tctccagtgc tgctgggcga
     9961 tttcgtgacg ttggagcagg gaactggctg tgtgcacatt gccccaggcc atggcatgga
    10021 agactacatc cttgtgctcg agcacaacgc caaggcctcg ccgggtgaac ggcttgaaat
    10081 tctcgctccg gtcgacgacg ccggtcggtt tactcaggcg gttcccgaat ttgcggggca
    10141 gcatgtcttc aaggcaaatc cgggcatcgt tgagctcctc cggacgaaag gccggctcat
    10201 cggccatggc tcgctggccc attcctatcc tcattgctgg cgctgtagga gccctgtgat
    10261 ctttcgcgct acggaacagt ggtttgtttc gatggacatc aatgctcttc ggcaagaggc
    10321 cttggcagag attgaccgtg tgaaatggat tccgccttgg gggaaggacc ggatcaacgg
    10381 gatgattgcg aaccgacctg attggtgcct ctcacgccag cgggtctggg gcgtgcccat
    10441 tccggcattc acctgtaccg gctgccggcg cgtcctcgcc gatcccagga tcattgacca
    10501 tatcgggtcg atcatggagg agcatggtgc cgacgtgtgg ttcaagcggc ctgctgcaga
    10561 attgttgccg gcggggacga tctgtccaca ctgcaacggg aaccagtttg aaaaggaacg
    10621 cgacattctt gatgtctggt ttgagtccgg tgtgagcttc gcggctgttc taaagccatg
    10681 ccggtggtgg ccggcggact tatatctgga aggctccgat cagcatcggg ggtggttcca
    10741 tagcgcgctc ctggctggca tcgtcacgga tcatcgcgcg ccgtatgagg ccgtgctgac
    10801 gcacgggttt gtccttgacg ggcaggggaa gaagatgtcg aagtcggcgg ggaatgtcgt
    10861 tgctccgcag gacgtcatca cacaatccgg cgcggaaatt ctgcgcctct gggtatccgc
    10921 gcaggactat cgggacgacg tgcgcatctc atcggagata cttaatcacc tcatagaagc
    10981 ctatagaaaa atccgcaaca cctgccggtt tttgctcagc aatttatacg atttcgatcc
    11041 ggcgacgcac cgcgtgccct acgaccaatt acccgaactt gatcgttggg ccctcatgcg
    11101 gttggaggaa atcaagacga aggtccgccg tgcctacgat gagttcgaat ttcatgccat
    11161 ctatcatgcg ctgaacaact attgctcagt cgatttgagc gcagtctatc tcgacatcct
    11221 caaggaccgg ttgtatacgt ttcgcgccgg tgatccgtta cgacggggtt cccaaaccgt
    11281 tctgtatgag atcatcgtcg ccgtcacgaa actcatggcg ccaatattga gtttcacggc
    11341 ggaggaaatc tggctgtcac tcggcgtgca acgcggcggg cactccagcg tgcatcttgg
    11401 tcaatttccc gaggcgtctc ccggatggca tgatgcgaaa ctggcgcaac ggtgggagcg
    11461 gctcctggca tatcggacgc aggtccaggg cgtgttagag gtcagccgac gagacaaggt
    11521 gatcggatcc tcgctggagg ctgcgattga aatacgggcg gatgccgatg cgttggcgtt
    11581 cttgaagacc tatgagcggg atcttccgac cttgttcatt gtgtcgcacg tgcgtctgac
    11641 tgctatatcc ggcggtcctg ccacggtagt ggccacgaag tcgtcatttg caaagtgcga
    11701 gcgctgctgg aactatcgtg aagcggtcgg gaaagatgcg gctcatccca cgctctgcga
    11761 ccgttgtgtg gaggccctcc gatgacgcga agctcccggt atctcgtcct ggcactgatc
    11821 ggagtcgtcg tgatcgtgct ggatcagatc acgaaatact gggtgatgca atccatgcgt
    11881 ctgcatgagt cgattccgat cgttcccaac ctgttcagtt ttacgtacat tcgtaaccct
    11941 ggtgcggcat tcggtttgct ggccggaagt agtaatgcct tccgcaccgt ctttttcgga
    12001 atcacgtcct tatttgccct ggcgctccta ggaactatcc tccttcgatt gccggaaaag
    12061 gattggatcg gccaacttag tatcgccggc attcttgggg gagcgatcgg caatttgatt
    12121 gatcggctcc gtttcggcga agtgatcgat ttcctcgacg tctatgttga gtcttatcac
    12181 tggcccgcgt tcaacgtggc tgactccgca atcagcgtcg gcgtagtttg cctgatcatc
    12241 cacttcgcgt tcgagcggaa agaggcgccg ttgcctgcgc cggatactcc tccaccgacc
    12301 ggctgctgaa aacagagtcg ttcgtccctg ccacagcgcg ccgaaccctg tttgcgaggg
    12361 aaagcacgcc catcaga
//
LOCUS       DASSCQ010000028        20258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_555572, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000028 DASSCQ010000000
VERSION     DASSCQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 20258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20258
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555572"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..896
                     /locus_tag="VFH05_02015"
     CDS             129..896
                     /locus_tag="VFH05_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HET6677018.1"
                     /translation="MATTAQDTREKIIVPGPAGFHPPSAAQLGVSLPDPGQGLFYGLL
                     EPNEEKVIEEMARKMLTSPNATIFPGPLLLWAWNDHAVEKAKATLEIAAQIPEVMIIP
                     MPDYRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCC
                     TMAICAEQGHEDAMLTIRDSDTAKIKRVAQIFKKVREEMGIKLPENGENVRFTGTQSK
                     VHGGKTHTNPVTFAPSPTGTGSAALFGHSAEHMKREG"
     gene            939..2282
                     /locus_tag="VFH05_02020"
     CDS             939..2282
                     /locus_tag="VFH05_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET6677019.1"
                     /translation="MSEALEAQQKTNPQGDPITSVAAPKADGKKDPHFEAKRQKIVTP
                     EYMFHEAPRTKEFITGSEAAKEAIRRSNVDLAVAYPITPQSETMQLVGVLYGEGYVKE
                     YYRGEEEVGVMAAIAGGSRAGVRCFTATAGPGTLRGMEGIASWPGHRLPAVAMFTCRV
                     VNAPLAIQPDNIEVAYLLNCGMIVFHAENQQDMFDFIMAGFTISEKNDVTLPVGVCCD
                     GFFVTHARGYVRMQDRGIKLPPREAWRGAVPVLDAENPPARLSRDAPVQKSNFMAYNI
                     HAVWQQEVWAAVERSRKYISRYMGGMLTAENVEGADAVIIASGSAAAQSREAVRLCKD
                     KGLKVGLIKVRSLRPFPTLELRQLCTQVKLIVVPEFNYVGWLAKEVATAIYGYSKAKI
                     IGGPRVYGGQSMPVELIVDEVESGLTGKKSTNVAMSSIMGGAVNPDEVAHFMRSI"
     gene            complement(2361..2609)
                     /locus_tag="VFH05_02025"
     CDS             complement(2361..2609)
                     /locus_tag="VFH05_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677020.1"
                     /translation="MNDPIVQAYKDVETAVERYTKLLQDHVLMLQNTEAPGSDKVVRM
                     TQGSKAMRDSAMIYLSYAKYVAYGMPASEEMVEDEIQG"
     gene            2647..3837
                     /locus_tag="VFH05_02030"
     CDS             2647..3837
                     /locus_tag="VFH05_02030"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HET6677021.1"
                     /translation="MTAFQAKGAARLSPLLSRILEAIEPEQSDVGTAVAELSRLFTKE
                     REALGTAYFDDPSLARAYLTYFFPVNLSKIQVLLDELPEDWHKAEGNRPLRVLDLGSG
                     PGTGALAVLDWLNQHDPDWLKQLTVVAVDASADALRQARHVWDAYARQGHISDARLIL
                     HEGDLARPTSGWRDHAVQSGPYDLIIVANCLNELFGRARHSLKEKSGLTGELLGLLGP
                     HGTLMLLEPALRETSRALHQVRDLLLVEKRCTVYSPCLHDGNCPALANPKDWCHEERA
                     WETPASIQRIDQQVGFIKDALKFSYLLLRTDGRTIVERSPHTFRMVSELRKLKGDTRA
                     WLCNELGRCEVGRLDRAETDVNLGWDECQRGTIVRIEHITRKDGASLGRIADRSAVHI
                     VRSV"
     gene            3857..4519
                     /locus_tag="VFH05_02035"
     CDS             3857..4519
                     /locus_tag="VFH05_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET6677022.1"
                     /translation="MSFITPDYFIHAANILLLVAYSVRDVLWLRLFAVAASLIAIPFY
                     LFQPTVLWAPLAWSVVFAAINLFQSWLLFVERRPVKLTAEEEEISRLAFQGLPPRKVL
                     QVLSIGSWISADVGEHLIERNKLPDAISLITRGKVQLSKDGHVLGDLIPGDIVGSALI
                     LNGIPSNVDAVTVEPLRAMRWQIGTLQKYLSANPETRAVLQRHLARDLAGKIGRLVTE
                     PS"
     gene            complement(4894..5076)
                     /locus_tag="VFH05_02040"
     CDS             complement(4894..5076)
                     /locus_tag="VFH05_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677023.1"
                     /translation="MSEQPKQPKPSEEPEQPKARKEIPLISVPNDRDMIAEEMEEMFE
                     EDKVNHQHGSSEPEAD"
     gene            complement(5141..5500)
                     /locus_tag="VFH05_02045"
     CDS             complement(5141..5500)
                     /locus_tag="VFH05_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677024.1"
                     /translation="MPAPTVQEVEDRLASVQCAVCKGSSFGIDQRFMQADGEWRGICK
                     KCFYSFPVYTDMEFYLRTQPDVPYRLREISCTVCKHRGVSLDFRATMSVRESIYFVTC
                     GGCKRQFPEKSSLEAFE"
     gene            5612..6028
                     /locus_tag="VFH05_02050"
     CDS             5612..6028
                     /locus_tag="VFH05_02050"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HET6677025.1"
                     /translation="MYQITDTLYVGNILEAERPPAHIEAVLLVAEEFTPNAPAGVIYE
                     RIPLKEFGEAKPASLNQAIDWIARHHPDKHVMVCCRAGMGRSVSVVMTYLCCIQKMEY
                     PDVFQLIMSRRPGACPLPNIQATIKQVHELRQARRG"
     gene            6086..6988
                     /gene="mutM"
                     /locus_tag="VFH05_02055"
     CDS             6086..6988
                     /gene="mutM"
                     /locus_tag="VFH05_02055"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET6677026.1"
                     /translation="MPELPEAEVVARQIRARLVGARLTGSWFGRPDIVREGYRDRSWY
                     AGSVLRAVERFGKSVVLRLEHQEEIRYIVAELGMTGLLLFPAAQTRFPQHVHVRLTFT
                     GGAESELRYWNPRRFGRISLFDQSGLERYLTRRFGCDPLTVTSEKFLTIIKERSGRLK
                     ALMMHQQAIAGIGNIYANEILFRARLHPDVLASRLPVAKVEELFRITREVLLEAISCG
                     GSSVRDFFAPDGSEGRFKGRHLVYGKEGLPCPNGCGRVIRRLQSERSSFICPACQART
                     PRRQRTSSPAPKNNSDLERSLTTA"
     gene            7108..8307
                     /locus_tag="VFH05_02060"
     CDS             7108..8307
                     /locus_tag="VFH05_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HET6677027.1"
                     /translation="MAKVLEGPGMGLMKKWGIHVPHYAVVTSADELAKLGHANDWMKH
                     TKLVAKAHEALGSRFKLGLVKVGLDLSAAIAATKEMIGRQVGSINVSQVIVSEMVAHK
                     EEYYCAVKSTREGSEVLVANCGGIEVESNWDRVKRLCLEVGQQPTAEALEKLVKDAGF
                     TGPLVKKMADFAAKMFTCFDNEDAQYLEVNPVVIRESDGELVALDAVTLLDGDAKFRH
                     PDWNFQFAAEFGRAYSKDEMEVMAVDSKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIKNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKSDGKLKNVRIWVRRGGPREKEGLDAMRALKDEGFDINVFDRNTPLTDIV
                     DKALHKQ"
     gene            8394..10214
                     /locus_tag="VFH05_02065"
     CDS             8394..10214
                     /locus_tag="VFH05_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET6677028.1"
                     /translation="MSILANKDTRVVIQGGQAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDAGKTNEVPYGSGLIAIPVYKTIAEATKNHPSINTSLVYIGADRAMKGGMEALDDP
                     HIKVVSMITEGVPEKDAKLLGAHARKLGKVFNGPSSIGIISAGSCRLGVIGGAFDNLV
                     LSKLYREGSFGVVTKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRIKLIGVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKAGALVPATFGALGPAIKETYQELLKSGQVKEPVEPTVLP
                     KLPKSVEEAMKADEVMVAPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGLIMIGPRF
                     GGAVTDAGRWFKYAVDNKMNVDEFLAYMKKHVGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLNMKTPCLDFALQVEQVTAAKKDNLILNVDGTMAAVLVDLGFPVDSLNGFFILSR
                     TIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            10299..12542
                     /locus_tag="VFH05_02070"
     CDS             10299..12542
                     /locus_tag="VFH05_02070"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET6677029.1"
                     /translation="MSMDLAKKLYAKMPDACAKARAKFGRGLTLAEKILVSHADNLET
                     QTWERGKAMLALRPDRVAMQDATAQMAILQFMQAGKKKVAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENKEVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGCLIIGTDSHT
                     PNGGGLGGLAIGVGGADAGEVMAGLPWEVLHPKLIGVKLTGKLSGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDQKMVAYMKITD
                     RSDLAELALANKTLLVADPEVYQSPEKHYDQIVEVDLSTLEPHVVGPHTPDLARPISK
                     LAAEAQEKGYPVELKAALIGSCTNSSYEDISRSAHVAKQGLKAGLKAKTSFLVSPGSE
                     RIYHTMKRDGFLDTFEQLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGINETLSFLASPEVVTAYALTGDLRFDPVNQKLKSADGKDFKLEPPVGEELPAKGF
                     AKGEEGFVAPAENGDQLTVDIPPTSERLQLLQPFPRWDGKDLEKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANSAFASEPGKGTNVLTGESNLTIAQIARAYKS
                     KGIGSIVVGDENYGEGSSREHAAMSPRFLNVRAVITKSFARIHETNLKKQGILPLTFA
                     DPKDYDKIEMNDRLSVVDLANLAPGKPVTVIIHKASGDVKIQANHSMTAQQITWFKAG
                     SALNALN"
     gene            12569..14797
                     /locus_tag="VFH05_02075"
     CDS             12569..14797
                     /locus_tag="VFH05_02075"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET6677030.1"
                     /translation="MAKADKIIYTKTDEAPMLATYSFLPIINAFSKAAGVTVELRDIS
                     LAGRVLAVFPDYLTPEQKQPDALAELGELAKTPEANIIKLPNISASMPQLVATIKELQ
                     SQGYKLPDYPENPKDDKEKEIKARYDKVKGSAVNPVLREGNSDRRAPLSVKAHTRKHP
                     HKMGPWSSDSKTHVSHMTGGDFRSNEKSVTIPAATTAKIEFVGADGKVTLLKDKVALQ
                     AGEVLDATFMSVKALRKFIEEQIEDAKKKGVLFSLHMKATMMKVSDPKIFGHAVTVFY
                     KDVFEKHGETFKKLGVDPDNGIGDLYAKIKSLPEDQRKAIEADIQEVYKKRPPMAMVN
                     SDKGITNLHVPSDVIIDASMPPVIRDSGKMWNPEGKLQDVKCVIPDSSYAPVYHEVIE
                     FCKKHGAFDPRTMGSVPNVGLMAQAAEEYGSHDKTFKAPGNGTIRIVDASGKTLLEHK
                     VEEGDIWRACQVKDTPIQDWVKLAVTRAKATGAPAVFWLNKDRAHDAELIKKVNAYLP
                     KHDTGGLEIKIMSPADACRFSLERMKDGKDTISCTGNVLRDYLTDLFPILEIGTSAKM
                     LSIVPLLNGGGLFETGAGGSAPKHVQQFQEEGYLRWDSLGEFLALAASLEHLAKVGNN
                     QVAKILADTLDQANAKFLESNKSPARKVGEIDNRGSHFYLALYWAQALAAQTADKKIA
                     ERFTKIAKDLSDNVAKIDQELLAAQGKPQDVGGYYHPNDEKAAKAMRPSATLNRIIDA
                     IA"
     gene            14829..15545
                     /locus_tag="VFH05_02080"
     CDS             14829..15545
                     /locus_tag="VFH05_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6677031.1"
                     /translation="MATESTDTNIIDQPEVLKPRMVTIEIAGKRVEVPEGITVIKAMW
                     YAGQEVVRGVGCLGGFCGACATYYRTKDDPKVRTCLACQTAVQDGMSFTMMPPFPARK
                     ATYDIQKLQDPKQDLFNLYPETPLCRNCNACTEACPQQIDVREGVWKAVFGDFKSVSE
                     MFMDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFTEKPVGLDARIKEITDGRFNDE
                     WNKILKMNDQQLAEHCATVK"
     gene            15631..17214
                     /locus_tag="VFH05_02085"
     CDS             15631..17214
                     /locus_tag="VFH05_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6677032.1"
                     /translation="MDIHALQQIVHSTRDTRRKQTIAKFSPADRDQLIHKYHPDYRAS
                     AYRPIRFGPNKGDRTVVELATLLEGDSPIHDDQDLTPHYTTDVLVIGGGGAGCAAALH
                     AHGAGSQVMLATKLRLGDSNSVMAQGGMQISVAPEDSPVTHFIDTLKGGHMQNDHALL
                     KVMVEDGPAIAKWLIELGVLFDRQADGNLHVKKGGGSSKPRLLTCSDYTGLEIMRVLK
                     DEVLNQKIQLLEFCAAVELLSDEDGRCSGAVFKDLDNQRHVVVAAKSVILATGGIGRL
                     HIQGFPTSNHYGATGDGLCLSYRIGAKLAHVDTFQYHPSGAVYPEQLIGALVTEGIRS
                     EGGHLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRVGVWLDTPLLDAEHG
                     PGTVEKHFPAMMMQFERFGIDISKDPVLIYPTLHYQNGGVKIDTNSESDVKNLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRSGLTAAARTASMKQGKLTLRHLQQFRTEAKKHG
                     NPSGVISPMILPAYTRKVG"
     gene            17311..18489
                     /gene="sucC"
                     /locus_tag="VFH05_02090"
     CDS             17311..18489
                     /gene="sucC"
                     /locus_tag="VFH05_02090"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET6677033.1"
                     /translation="MNVHEFQAKSLFAQFGVPVPRGKEITSPEAATAWADELNTPVFV
                     VKAQIHAGGRGKAGGVKITKDKAAVAGLAKELIGKTLITHQTGPKGRKVHRLLMEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAEKTPDKIIKEAIDPAVGFQGHNG
                     RNVAFALGLQNIEPAVINPFVQMLGNLYRLFMDKHAALVEINPLIITKEKTLVALDGK
                     VSFDDNGLFKHEDVQKMRDLNEEEPLEIEATANNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKETVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKINVPLVVRLQGTNADEGRKLLAESGLKLEVANDLWEAAQKIVRLTGKAA
                     "
     gene            18755..19627
                     /gene="sucD"
                     /locus_tag="VFH05_02095"
     CDS             18755..19627
                     /gene="sucD"
                     /locus_tag="VFH05_02095"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET6677034.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKVVAGVTPGKA
                     GQEVEGIPVFNTVADAVKKTESDTSLIFVPPPFCADAILEAADAGIKLIICITEGIPV
                     NEMVRVKRALRGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKKGIVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDPETQAIVMIGEIGGDAEEKA
                     AEYIKKHVKKPVVSFIAGITAPPGRRMGHAGAIISGGKGTATEKMKALEAAGVRVIKN
                     PAEIGITVKKALGR"
     gene            19796..>20258
                     /locus_tag="VFH05_02100"
     CDS             19796..>20258
                     /locus_tag="VFH05_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET6677035.1"
                     /translation="MRVFLVHVRDPQFYALPAKTRAKNGNIRVMGFPPIGIMSLSAVL
                     KEAGHECVMFDQANPDTPNHVIVDEIKKQKPALIGLSFLSTTSYPYAKILARQIRAAD
                     STVKLAFGGVFASLNAGLVKLQCPEVDFVCRGDGEQLLLDLLTQIDAPETVG"
BASE COUNT         4550 a         5602 c         6033 g         4073 t
ORIGIN      
        1 atgatcatga tcgatcagct gcccaaggcg gcactgaccg acaatgtgac ctacggtgag
       61 aagagcacca ctgcggtgct cgccagcccg gttgtcggat aagcgtcggg ttcagaaagg
      121 ggaacggagt ggccactaca gcgcaagaca cgagggaaaa aatcatcgtc cccggaccgg
      181 ccgggttcca tccgccgtcg gcggcccaac tcggcgtttc gttgccggat ccaggtcagg
      241 ggctgttcta cgggcttctt gagccgaacg aagaaaaagt catcgaagag atggcccgta
      301 agatgttgac cagtcccaat gccacgatct tccccggtcc actgctcctc tgggcgtgga
      361 acgaccacgc tgtcgaaaag gccaaggcga cgctcgagat cgcggcgcag attcctgaag
      421 tcatgatcat tcccatgccg gattacagac ccaagtatcc caagatcgac cccgaagaag
      481 tcatcaatcc gaaccatccc aatctgacta tttggggcaa caagatcgag gcctgcatct
      541 ttattggcgt gcactgccac tatgccaatt taacgctgaa gatgattcgg gccggaacca
      601 actgctgcac gatggccatc tgcgccgagc aaggccatga ggacgccatg ctgacgattc
      661 gcgattctga caccgccaag atcaagcggg tggcgcagat ttttaaaaag gtgcgcgagg
      721 agatgggtat caagcttccc gagaacggag aaaatgtccg ttttacaggg actcagtcaa
      781 aagtgcacgg cgggaaaacg cataccaatc ccgtgacatt tgctccatct ccgaccggaa
      841 ctggaagtgc cgcgctgttc ggtcattctg cagaacacat gaaacgcgaa ggataggccg
      901 agcaaacggc gctcaccact ttttaacgag gtgcgatcat gagtgaagct ctcgaggctc
      961 aacaaaaaac caacccccag ggcgacccga ttaccagcgt tgccgctcca aaagcggatg
     1021 ggaaaaaaga tccgcatttc gaggccaagc ggcaaaagat cgtgaccccg gaatacatgt
     1081 tccatgaagc gccccggacc aaagaattca ttaccggcag cgaggcggcc aaagaggcga
     1141 tccggcgttc gaacgtcgat ctcgctgtgg catatccgat cacgccgcag agcgaaacca
     1201 tgcagttggt cggagtgctc tatggtgaag gctacgtgaa ggaatattac cgcggggaag
     1261 aagaggtggg cgtgatggct gcgattgccg gcggctcgcg tgccggcgtg cgatgcttta
     1321 cggcgactgc tggtcctggg acattgcggg ggatggaagg cattgcctca tggcccggcc
     1381 atcggttgcc tgctgtggcc atgttcacct gtcgtgtcgt caatgcgccc ctggccatcc
     1441 agccggacaa tatcgaggtg gcctatctgt tgaattgcgg catgatcgtc tttcatgcag
     1501 agaatcagca ggacatgttc gatttcatca tggccggctt tacaatcagc gaaaaaaacg
     1561 atgtgacgtt gcctgtcggc gtctgctgtg acggcttctt cgtcacgcat gcgcgcggct
     1621 acgtgcgcat gcaggaccgc ggtatcaagc tccccccacg cgaagcctgg cgaggggccg
     1681 ttcctgtgct cgatgcggag aatccccccg cccgactctc gcgcgacgcc ccggtgcaaa
     1741 aatccaactt catggcctac aacatccatg cggtgtggca acaggaagtg tgggcggccg
     1801 tggagcgttc ccgcaaatac attagtcgat acatgggcgg catgctgacc gcggaaaacg
     1861 tggaaggcgc cgacgccgtc atcatcgcgt ccggcagcgc ggccgctcag tcgcgggaag
     1921 cggttcgcct ttgcaaggac aaaggcctca aggtcggatt gatcaaggtc cgctcattac
     1981 gcccctttcc gactctggag ctccgtcagc tctgtacaca ggtcaaactc atcgtcgtgc
     2041 ccgagttcaa ctacgtcgga tggttggcaa aagaagtggc gaccgcgatc tacggctact
     2101 caaaggccaa gatcattggc ggcccgcgtg tctatggcgg ccagtcgatg ccggtggaat
     2161 tgattgtaga cgaggtcgaa tccggtctca ccgggaagaa atccaccaac gtcgcgatgt
     2221 cctcgatcat gggcggtgcc gtcaatccag atgaagtcgc tcatttcatg cgcagcatct
     2281 gagttctcgg gctgaaaact gaagagctcg tcaggatggc atcctgacga gctcttttca
     2341 tttctcgttc gtaccgcagc ctatccttgg atttcgtctt ccaccatttc ctcgctggca
     2401 ggcatgccat aggcgacata tttggcatag gacagataaa tcatggcgct gtcccgcatg
     2461 gctttcgatc cctgggtcat gcgcacgacc ttgtcggaac cgggggcttc cgtgttttgg
     2521 agcatcagga catggtcctg cagcaacttg gtatagcgct cgacggccgt ttcaacatcc
     2581 ttgtaggcct gcacaatcgg gtcgttcatg gttgtcctcc ctcgcaggct cagcatacac
     2641 tagggcatga cggctttcca agccaagggg gcggcccggc tctcacctct tctgtcacgg
     2701 attcttgagg ccatcgaacc ggagcagtcc gatgttggga cggccgttgc cgaactctcg
     2761 cgtctgttca cgaaagagcg cgaagcgctg ggcacggcct atttcgacga tccttccctg
     2821 gcacgggcgt atctcacata cttttttcct gtcaatcttt ccaaaatcca agtcctgctc
     2881 gacgaactgc cggaggactg gcacaaagct gaagggaatc ggccgcttcg cgtactcgat
     2941 ctcggatccg gaccaggcac gggtgccctg gcggttctcg actggctcaa ccagcatgat
     3001 cctgattggc tcaagcaact gaccgtggtt gccgtcgatg cctcagcgga cgctttgcgc
     3061 caggcccgac acgtgtggga cgcctatgcg cgccaggggc atatcagtga tgcgaggctg
     3121 atcctccatg aaggtgatct cgcgcgtcca acgagcggct ggcgggatca tgctgttcaa
     3181 agtgggccct acgatctcat tatcgtggca aactgcctca acgaattgtt cggccgagca
     3241 cgccattcct tgaaggagaa atccgggcta acaggtgagc ttcttgggct gctcgggccc
     3301 catgggaccc tgatgctcct cgagcctgcg ttacgcgaaa cgtcacgggc gttgcatcaa
     3361 gtccgtgatc tcctacttgt cgaaaagcgg tgcaccgtct acagtccatg cctccatgac
     3421 ggaaattgtc ccgccctggc taaccctaag gattggtgtc atgaggagcg tgcctgggag
     3481 actcccgcgt ctatccagcg gatcgatcag caagtggggt ttatcaaaga cgccctgaaa
     3541 ttctcctatc tgctgttgag aaccgacggg cggacaatcg tcgagcgcag tccacacact
     3601 ttccggatgg tcagtgaatt acgcaagctc aaaggcgata caagggcctg gctgtgtaat
     3661 gaacttggtc gatgtgaggt ggggagactg gaccgcgcag aaaccgacgt gaacttaggt
     3721 tgggacgaat gtcagcgggg gaccatcgtg cggattgaac atatcacgcg caaggatggg
     3781 gcgtcgttgg gaagaattgc cgaccgctcg gcggtccaca tcgtgcgttc cgtatgagca
     3841 ctgtccaagg agaaatatga gcttcatcac ccctgattat ttcatacacg ccgccaacat
     3901 cctcttactg gttgcgtaca gcgttcgcga cgtcctgtgg ttgcgcttgt tcgcggtggc
     3961 agcttccctc attgccattc ctttttatct gtttcagcct accgtgttgt gggcgccgct
     4021 tgcttggagc gtggtctttg cagcgatcaa tctctttcaa tcgtggctgc ttttcgtcga
     4081 gcgtcgacca gtcaagctga cggccgagga ggaggaaatt agccgacttg cctttcaggg
     4141 attacctcct cggaaagtct tgcaggtgct gagcatagga tcctggatct cggcggacgt
     4201 tggcgaacac ctaatcgaac gaaataagct tcccgacgcc atttcgctca tcacgcgtgg
     4261 caaagtgcag ttgtcgaaag atggccacgt gctcggggat ttgattccag gtgacatcgt
     4321 gggctccgct ctcatcctca acggcatccc gtccaatgtg gatgcggtga cagttgagcc
     4381 cctgcgcgcc atgcgttggc agattgggac attgcagaag tatctcagcg ccaaccccga
     4441 gacgcgtgcc gtcttgcagc ggcatctcgc ccgagatctt gccggaaaga tcgggcgcct
     4501 cgtcacggag ccctcctaaa cctcagcctg acatgttgcc ggccgattat catcggttga
     4561 tcgcagcttt ttcggttccg agcagacggg atcgccgccg gatcgcttct atcacatggc
     4621 cgaagcatcg aattgattct acgtagagca gcgctgaccg gttctgtctg cgattcgtga
     4681 aggcgaggga caccgcgttt gccgaaaggc tgcggtccga agcagaagga cggcggcgca
     4741 tgtgtgaagt tcaggtcgag cgcagcgggg cctgaacgag ttgcgacgcc gagcaggctg
     4801 aggcgtagcg cggtcgaggc gaagcgttga gcagagcaga actgtcagtg cgcgttaaaa
     4861 tgttttaatc gaatcagaga tggattgctt gaatcaatcg gcttcgggtt cggaactgcc
     4921 gtgctggtgg ttcaccttgt cctcttcgaa catctcttcc atctcttccg cgatcatgtc
     4981 gcggtcgttc gggaccgaga tgagcgggat ctctttgcgg gctttcggct gctccggctc
     5041 ttcggatggt ttgggttgtt ttggctgctc actcatcagt agagtataca ccaggctcat
     5101 aggaggatgt tgaaaaagta gcccagcacc gtttggactc ttattcgaag gcttcgaggg
     5161 aggatttctc ggggaattgc cgcttgcaac cgccgcaggt cacaaaatag atcgactcgc
     5221 ggaccgacat cgttgcgcga aaatccaggc tcacccctcg gtgcttgcag accgtacagg
     5281 agatctctcg tagacggtag ggcacatcag gctgggtgcg gagatagaac tccatgtcgg
     5341 tgtagacagg gaagctgtag aagcacttct tacaaattcc ccgccattcg ccatcggcct
     5401 gcatgaaccg ctgatcgatg ccgaagctcg accctttgca gacagcacat tgaacggatg
     5461 ccaatcggtc ttcaacctct tgaaccgtcg gtgccggcat tcgattaccc tcttatttgg
     5521 agtatacgtt cgcgaacact cgactgcaac cggtgtcgtc aagctttctg cttgacctct
     5581 atgccggcct ctctaatact ggattgactt catgtaccaa attaccgata cgctttacgt
     5641 gggaaacatt ctggaagcgg agcggcctcc ggctcacatt gaggcggtgc ttctcgttgc
     5701 ggaggaattc accccgaatg cgccggccgg cgtcatctat gagagaattc cgctgaaaga
     5761 gttcggggaa gcgaagccgg cttccctgaa ccaggccatt gactggatcg cgcgccatca
     5821 tccggacaag catgtgatgg tctgctgcag ggccggaatg ggccgttcag tgtccgtggt
     5881 catgacctat ctctgttgca tccagaaaat ggagtacccg gatgtgttcc aactgatcat
     5941 gagtcgccgg ccaggggcct gtccgcttcc caacatccaa gcaaccatta agcaggtcca
     6001 tgaactccga caagcccggc gcggctagat cgccactacg ctctgcccat tgcctttttc
     6061 cccagccaat ggcctaatca gagtcatgcc tgaacttcct gaagccgaag ttgtcgcccg
     6121 gcagattcgc gcccgcttgg tcggggccag gttgacgggc agttggttcg gtcgacccga
     6181 tatcgttcgc gaaggctatc gcgatcgcag ttggtatgcg ggctcagtgt tacgtgctgt
     6241 cgagcggttc ggcaaaagcg tcgtgttgcg gttggaacac caggaggaaa tccgatacat
     6301 cgtggctgaa ctcgggatga cgggcctgct gctttttccg gccgcgcaga cgaggtttcc
     6361 ccagcacgtc catgtgcgac tgacctttac cggaggcgct gagtcggagc tccgttattg
     6421 gaatccgcgc cgattcggcc gaatctcgtt gttcgaccaa tcgggactcg agcgctatct
     6481 cactcggcgg ttcgggtgcg atcccttgac cgtcacatcc gaaaagtttc tcacgatcat
     6541 caaggaacgc tcagggcgct tgaaggcgct catgatgcat cagcaggcca tcgcgggaat
     6601 cgggaacatt tatgcgaacg agattctgtt ccgagcacgg ttgcaccctg acgttctcgc
     6661 gagtcgtctt cccgttgcca aggttgagga actcttccgg atcacgcgcg aggtactgct
     6721 tgaagcgatt tcctgcggtg gctcgagcgt gcgtgatttc tttgcgccgg acggcagtga
     6781 agggcgcttt aaaggccggc atctcgtcta tggaaaggaa gggcttccgt gcccgaacgg
     6841 ctgtggacga gttatcagac gccttcagag cgaacggagc tcatttatct gtccggcttg
     6901 ccaagcacgc acgcctcgtc gacagcgcac ttcatctccg gctcctaaga ataacagcga
     6961 cttagagcga tccctcacca ctgcctgaac ccctaggcct tttgatccct catcgcttag
     7021 ttcctctggt gtattgagtc atctacgccg tttcgctaca atccgtctcc ctttacatcg
     7081 attcttcata ccccaaggag cgacgccatg gccaaggtgc tggaaggtcc cgggatggga
     7141 ctgatgaaga aatggggcat tcatgtccct cactacgcag tcgtcacttc agccgacgaa
     7201 ctcgccaaac tcggtcatgc caacgactgg atgaaacaca cgaaactcgt ggccaaggcg
     7261 catgaagctc ttggctcacg ctttaagctt ggcctcgtga aggtcggact cgatctcagc
     7321 gccgcgattg ccgccacaaa ggagatgatt gggcgccaag tcggcagcat caacgtctca
     7381 caagtcattg tctcagagat ggtcgcgcac aaggaagaat actattgcgc cgtgaagtcc
     7441 acccgcgaag gcagcgaagt tctcgtggcc aattgcggag gtatcgaagt cgaatcgaac
     7501 tgggatcgag tgaagcgcct ctgtctcgag gtgggtcagc aacctacggc ggaagcactg
     7561 gagaagctgg tcaaggatgc cggatttaca ggcccgctcg tcaagaagat ggccgacttc
     7621 gccgccaaga tgttcacctg tttcgataac gaagacgccc aatacctgga agtgaacccc
     7681 gtcgtcatcc gtgaaagcga cggcgaattg gtggccctgg atgccgtgac gcttctggat
     7741 ggggacgcca agttccgcca tcccgattgg aatttccaat ttgcagctga attcggccgg
     7801 gcctacagta aggatgagat ggaagtcatg gccgtcgaca gcaaaatcaa gggatcggta
     7861 aaattcatcg aaataccggg tggcgatacc gcgatgcttc cggccggcgg cggtgccagc
     7921 gtctattatt cggacgcagt ggtggcgcgc ggaggtaaac tagccaatta tgccgagtat
     7981 tccggcgatc ctcccgactg ggccgtcgaa gtgctgaccg agaaagtctg ctcgctgccc
     8041 ggcatcaaga acatcatcgt gggcggtgcc attgccaatt ttacggacgt gaagaagacc
     8101 tttggcggca ttatcaatgg tttccgcaag gcgaaatcgg acggcaaatt aaagaacgta
     8161 cggatttggg tgcgccgcgg cggccctcga gagaaggaag gcctcgatgc catgcgggcg
     8221 ctgaaggatg aaggattcga catcaacgtc tttgaccgca acaccccgct gacggacatc
     8281 gtcgacaagg cgcttcacaa acagtaacga aggctggaac ggcctgaggc caggagcatt
     8341 cgcttggctc ccggtcacgg tattcagttt cgatcgaata aagggagatt ccgatgagca
     8401 tcctggctaa caaagacacc cgcgtcgtca ttcagggcgg gcaggccggt gtgaacgccg
     8461 cacgacgtat ggcggaattc tgctacctca tcaagcggcc gctgaatgtt gaagcgttcg
     8521 tctatccgcc ggatgccggc aaaaccaacg aagttcccta cggcagcggg ctcatcgcca
     8581 tccccgtcta caagaccatc gccgaagcga ccaagaatca tccctcgatc aatacaagcc
     8641 tggtgtacat cggtgcggac cgtgcgatga agggcggcat ggaagcgctt gacgacccgc
     8701 acatcaaggt cgtgtccatg atcaccgaag gcgttccgga gaaggacgcc aagctactgg
     8761 gcgcacatgc acggaaactc ggcaaggtgt ttaacggacc ctcttcgatc gggatcatct
     8821 cggccggctc ctgccggctg ggcgtgatcg gcggtgcgtt tgacaacctg gtgctctcga
     8881 aactgtatcg cgaaggatcc ttcggcgtcg tcacaaagtc aggcggcctt tccaacgaga
     8941 tcatctggat ctgttcacag tttgccgacg gcattacgac cgcaattggc atcggcggcg
     9001 acgcctatcc cggcaccgac tacgtgagct atttggagat gttcgaaaac gatccgcaaa
     9061 cgaaagccgt cgtgatcgtg ggcgagatgg ggggcgatct cgaggagagg gcggcggagt
     9121 ggtacggcgc gaaaaagcgc cgcatcaagc tgattggtgt cgtgtccggc ttctgtcagg
     9181 aaagtttacc gaagggtatg aagttcggcc atgcaggtgc caaggaaggc atgaaaggcg
     9241 agggttcggc acgctccaag tcggatgcac tcaagaaggc gggggccctt gtgccagcca
     9301 ctttcggtgc cttgggtcct gcgattaagg agacgtatca ggagctgctg aagtcaggcc
     9361 aggtcaagga accggtggaa ccgacggtgc tgccgaaact gccgaagtcg gtcgaggaag
     9421 ccatgaaagc ggacgaagtg atggtcgcgc cgctcatccg gaccaccatc agcgacgatc
     9481 gtggcgacga accctgctac gacgggtatc cggcctccga actgatcaac aaaggttacg
     9541 agattcccca tgtcatcggt ttgctctggg acaagcgact tatctcgaaa caggaagcgg
     9601 aaatcatcaa gcgcatcatg atgttgtcgg ccgatcacgg cccgtgcgtg agtggggcct
     9661 atgcgacgat cctggcggca tgcgccggca tcgggctgtc ccaatccgtg gccgcaggtc
     9721 tcatcatgat cggcccgcgt ttcggcgggg cagtgaccga cgccggtcgg tggttcaagt
     9781 atgccgtcga caacaaaatg aacgtggatg aatttctggc ctatatgaag aaacatgtcg
     9841 gtccggttcc ggggatcggc catcgagtga aaagcctgcg taatccggac aagcgcgtca
     9901 aagagctcgt tggctacgtc aagagtctca acatgaagac gccgtgcctc gattttgcgc
     9961 tgcaggtgga gcaggtcacg gccgcgaaga aggacaatct cattttgaat gtggacggca
    10021 ccatggcggc cgtcctggtc gatctgggat tccccgtgga cagtttgaac gggttcttca
    10081 ttctgtcgag gacgatcgga ttaatcggcc attgggtcga tcagaagcgc caggacagcc
    10141 ggttgattcg gctgtttgat tatctcgtca actatgcggc gcccaaacga agagaagtgc
    10201 cgccactgaa ataagtggtg aggcgctcgc gtgcgagcga gtgaatgagt tcaacgttcg
    10261 agatttgaaa atccacattc cacattgagg atgtcatcat gtcgatggac cttgcgaaaa
    10321 aactctatgc caaaatgccc gacgcctgcg ccaaggccag ggcgaagttc ggccgcgggc
    10381 tgacgttggc cgagaagatt ctcgtttctc acgccgacaa cttggagacc caaacctggg
    10441 agcgcggcaa ggccatgctg gctctgcgcc cggatcgcgt ggccatgcag gacgcgaccg
    10501 cgcaaatggc catcctgcag ttcatgcagg caggcaagaa gaaagtcgct gtgcctagca
    10561 ccatccactg cgatcacctg atccgggcag aaatgggttc ggaaaaagat ctcctgcgtg
    10621 cgatggacga gaataaggag gtctacaact tcctggcctc tgcggccaaa aaatacggga
    10681 tcgggttctg gaagcccggc gcggggatca tccaccaagt cgtcctggaa aattatgcgt
    10741 ttcccggctg cttaatcatt gggaccgact cccatacacc caacggcgga ggcctaggag
    10801 ggttggccat tggcgtcggc ggagccgatg ccggtgaagt gatggctggt ctgccctggg
    10861 aagtcctcca tcccaaactc atcggtgtca aattgacggg caaattgagc ggctgggcat
    10921 cgccgaaaga cgtgattctt tatctgtgcg gtctgctcac ggtgaagggc gggacgaata
    10981 agatcgtcga atacttcggt ccgggggcag aaaccatcag cgccactggg aagggcacaa
    11041 tttgcaacat gggcgccgag ctgggagcga ccacctccgt gttcccattc gatcagaaga
    11101 tggtcgccta catgaaaatc acagatcgat ccgatttggc cgagctggct ctggccaaca
    11161 agaccctcct tgttgccgat cccgaggtct atcaatcacc agaaaagcat tacgatcaga
    11221 tcgtggaagt ggacctctcc acgcttgagc cgcatgtcgt cggtccacat acgcctgatt
    11281 tagcccggcc gatctccaag cttgcggccg aggctcagga gaaggggtat ccggtagagt
    11341 tgaaagctgc gttaatcggc agctgtacca attcctcgta tgaggacatc agccggtccg
    11401 cgcatgttgc gaagcagggc ttgaaagccg gcttgaaggc aaaaacctcg ttcctggtat
    11461 cgccgggatc agagcggatt tatcacacga tgaagcgcga cgggtttttg gacaccttcg
    11521 aacagctcgg cggcacggtg ttgtcgaact cctgcgggcc ctgcatcggg caatggaagc
    11581 gggccgatgg ggtgaaaggc aaggccgatt cgatcgtcag ttcgttcaac cgcaatttcc
    11641 ccggccgcaa tgacggcatc aacgagactc tgtcgtttct tgcaagtccg gaagtcgtga
    11701 cggcctatgc cctcacgggg gacctgcgtt tcgaccccgt caaccaaaaa ctcaagagtg
    11761 ctgacggcaa ggatttcaag ctcgagcccc cggtgggtga ggaactgccg gcgaagggat
    11821 ttgccaaggg cgaggagggc ttcgtcgctc cggccgagaa tggcgatcaa ctgaccgtcg
    11881 acattccgcc gaccagcgag cgcttgcagt tgttgcagcc gttcccgcgc tgggacggga
    11941 aagacctcga gaagttgccg ctgttgatta agacgaaggg caagaccacc accgatcaca
    12001 tttcgcccgc cggcccctgg ctgaagttca gagggcattt ggacaagatc agcgacaaca
    12061 tgtttctggg cgctaacagc gcattcgctt cggagccagg caagggcacg aacgtcctga
    12121 ccggcgaatc gaacctcacg attgcgcaga tcgcgcgcgc gtataaatcc aaagggatcg
    12181 ggtcgatcgt cgtcggcgat gagaattacg gcgagggcag cagccgcgag cacgcggcga
    12241 tgtcgccgcg ctttttgaac gtccgcgcgg tcatcacgaa aagtttcgcg cgcattcacg
    12301 agaccaattt gaagaagcag ggcattctcc cgttgacttt cgcagatccc aaggactacg
    12361 acaagatcga gatgaacgac cggctgagcg tcgttgatct ggccaacctg gcgccgggga
    12421 agccggtcac ggtcattatt cacaaggcga gcggtgatgt gaagattcaa gccaaccaca
    12481 gcatgaccgc acagcaaatc acctggttca aggccggttc agcgttgaac gcgctgaact
    12541 gaaaccagcg actaaagagg gacatatcat ggcaaaagca gacaagatca tctatacgaa
    12601 gaccgacgaa gcgccgatgc tggcgaccta ttcctttctc ccgattatca atgccttttc
    12661 gaaggcggcg ggtgtgaccg tggaattgcg tgacatctca ctcgccggcc gcgtcctcgc
    12721 cgtctttcct gactatttaa cgccggagca gaagcagcct gatgcgctgg cagagctcgg
    12781 ggaactggcc aagacgcccg aagccaacat tatcaagctg ccgaacatca gcgcctcgat
    12841 gccgcagctg gtggccacga tcaaggaatt gcagagccag gggtacaagc tgccggacta
    12901 cccggagaat ccaaaggacg ataaggaaaa agagatcaag gcccgctatg acaaggtgaa
    12961 aggcagcgcg gtcaatcccg tgctccgcga aggtaactcc gatcgccgcg ccccgctttc
    13021 cgtgaaggcg catacccgga agcatccgca caagatgggg ccatggagtt ccgattccaa
    13081 gacccatgtt tcgcacatga cgggcggcga tttccggtcg aacgagaagt ccgtgacgat
    13141 cccggcagcg accacggcca agatcgagtt cgtgggagca gatggcaagg tcaccctgtt
    13201 gaaagacaaa gttgccttgc aggccggtga agttctcgac gccacgttca tgagcgtgaa
    13261 ggcgctgcgc aagttcatcg aggagcagat cgaggatgcc aaaaagaagg gcgtcctgtt
    13321 ttctctgcac atgaaggcca cgatgatgaa ggtctccgat ccgaagatct tcggccatgc
    13381 ggtaaccgtc ttctacaagg acgtatttga aaagcatggt gagacgttca agaagctcgg
    13441 cgtggatccg gacaacggga tcggcgacct gtatgcgaag atcaagtcac tgcctgaaga
    13501 tcagcgcaag gcgatcgagg ccgacattca ggaggtgtac aagaagcggc caccgatggc
    13561 catggtcaac tcggacaagg gcattacgaa tctgcacgtg ccgagcgacg tcatcatcga
    13621 cgcatcgatg ccgccggtca tccgcgactc tggcaaaatg tggaatccgg aaggcaagct
    13681 gcaggacgtg aaatgcgtga ttccagactc cagttatgcg cccgtctacc atgaagtgat
    13741 cgagttttgt aagaagcatg gcgccttcga ccctcgaaca atgggcagtg tgcccaacgt
    13801 cggcttgatg gcccaggcag cggaagaata cggctcccac gacaagacct tcaaggcgcc
    13861 cggcaacgga accatccgga tcgtcgatgc ctcaggcaag acgttgctgg agcacaaggt
    13921 ggaagaaggc gatatctggc gcgcctgtca ggtcaaagat actccgattc aggactgggt
    13981 caaactggcg gtcacccgcg ccaaggcgac cggcgctccc gccgtcttct ggctgaacaa
    14041 ggaccgggcg cacgatgccg agttgatcaa gaaagtgaat gcctatctcc cgaagcatga
    14101 tacgggcgga ctcgaaatca agatcatgtc tccggctgat gcctgccgct tttccctgga
    14161 gcggatgaag gacggcaagg acacaatttc ctgcaccggt aacgtgcttc gtgactatct
    14221 gaccgacttg tttccgattt tggaaatcgg cacgagcgcc aagatgctct cgatcgttcc
    14281 gcttctcaac ggcggcggct tgtttgaaac gggcgcgggc ggttcggcgc cgaagcacgt
    14341 tcagcagttc caagaggagg gctatctgcg atgggactcg ctgggcgagt tcctggcgct
    14401 cgcggcctcg ctcgagcacc tggcgaaggt gggaaacaac caggtggcca agatcctcgc
    14461 cgatacgctg gaccaagcca acgcaaagtt cctcgagagc aacaagtcgc cggctcgtaa
    14521 agtaggtgag atcgacaacc ggggcagcca tttctacctg gcgctgtact gggcgcaagc
    14581 cctggctgca cagacggccg ataaaaagat cgcagagagg tttacgaaga tcgccaaaga
    14641 cctgagcgac aatgttgcaa agatcgatca agagttgctg gcggcgcaag gcaagcctca
    14701 ggatgtcggc gggtactatc atccgaacga tgagaaggcg gccaaggcca tgcgtcctag
    14761 tgccacgttg aatcggatca ttgacgcaat tgcataacac tcgccgagcg gcggggctgt
    14821 gagactgtat ggcaacagaa agcaccgata caaacatcat cgaccaaccc gaggtgttga
    14881 agccccgcat ggtgaccatc gagatcgccg ggaagagggt ggaagtgccg gagggcatca
    14941 ccgtcatcaa ggccatgtgg tacgccggtc aggaggtcgt gcggggtgtc ggctgtctcg
    15001 gcggcttctg cggggcctgt gcgacctact atcggacgaa agacgatccg aaggtgcgca
    15061 cctgtctcgc atgtcagacg gcagtacagg acggcatgtc attcaccatg atgccgccct
    15121 tccccgcccg caaagccacg tacgacattc aaaaacttca ggaccccaaa caagatctct
    15181 tcaatctcta tcctgagacg ccgctctgcc ggaattgcaa tgcctgcacg gaagcctgcc
    15241 cgcagcagat cgacgtgcgg gaaggcgtat ggaaagcggt attcggcgac tttaagagcg
    15301 tgtccgaaat gttcatggac tgcgtcatgt gcgggatgtg tacgccggtc tgcatcgccg
    15361 acatcgctcc gaacctggtc gcgctctatg tgagccgcgc tcagggcgct catttcaccg
    15421 aaaagccggt gggcttggac gcccggatca aggaaatcac agacggtcgg ttcaatgacg
    15481 agtggaataa gattctcaag atgaacgacc agcaacttgc cgagcactgt gccacggtga
    15541 agtgacaagc actcggccaa cggtaccgtc ctatgcgcga cgcgcccggt gtgttcatga
    15601 cgaccgctga ctattgacgg tcttgaagag atggatatcc acgcacttca acagattgtt
    15661 cacagcacga gggatacccg tcggaaacag accattgcaa aattttctcc cgccgaccgc
    15721 gatcagctga ttcacaaata tcatcccgac tatcgagcca gcgcctaccg gccgattcga
    15781 ttcggtccga acaaagggga ccggacggtg gtcgagttgg cgacgttgct tgagggtgac
    15841 agcccaatcc acgacgacca ggacctgact ccgcactata cgaccgatgt cctcgtgatc
    15901 ggcggcggcg gagccggatg tgccgcggcg ttgcatgcgc acggagccgg atcgcaggtc
    15961 atgctggcca cgaagcttcg gctgggtgat tcgaacagtg tcatggcgca gggtgggatg
    16021 caaatctccg tggcgccaga agattctcct gtcacccact tcatcgatac gcttaagggc
    16081 ggccatatgc agaacgacca tgccctcctg aaggtgatgg tcgaagacgg gccggccatt
    16141 gccaagtggc tgatcgaact gggcgtgctc ttcgaccgtc aggccgacgg gaatctccat
    16201 gtgaagaagg gtggcggcag ctcgaagcca cggctgttga cctgctccga ctacacgggc
    16261 ctcgagatca tgcgcgtcct caaagacgaa gtgctcaacc aaaaaattca gctgctcgaa
    16321 ttctgcgcgg cggtcgaatt gctgagcgac gaggacggcc gctgttcagg cgcggttttc
    16381 aaggatctcg acaaccagcg gcatgtggtc gtggccgcga agtccgtgat cctggccacc
    16441 ggtggcatcg gccggctgca cattcaaggc ttcccgacca gcaaccatta tggtgcgacg
    16501 ggagacggac tgtgcctttc ctaccgcatc ggcgcgaagc tggcgcatgt cgacacgttc
    16561 caataccatc cgtccggcgc agtctatccg gaacaattga tcggagcatt agtgacggag
    16621 ggtattcgct ctgagggcgg tcatttggtg aatgcgaagg gggagcggtt tgtcaatgaa
    16681 ctcgacacac gcgacgtcgt atcttcctcg atcattcgag aatgcgaaga aggccgaggc
    16741 atcagaacca tgtcgggaag ggtcggcgtg tggctggata cccctctcct ggatgcggaa
    16801 cacgggccgg ggacggtcga gaagcatttt ccggccatga tgatgcagtt cgaacgattt
    16861 gggatcgaca tcagcaagga tccggtgttg atctatccga cattgcacta ccagaacggc
    16921 ggtgtgaaga tcgacaccaa ttcggaaagc gacgtgaaga atctgtttgt cgccggggaa
    16981 gcctccggcg gcctccacgg ccgcaaccgg ttaatgggaa attcactgct cgatctcatg
    17041 gtgttcggca agcggtccgg tctcacggcg gctgcacgaa ccgcgtccat gaagcagggg
    17101 aaacttacgc tcagacattt gcagcagttt cgtacggaag ccaagaagca cggaaatccc
    17161 agtggcgtca tatctccgat gatcctgccg gcgtacacga gaaaagtggg gtaggctacg
    17221 accgcaacgt gctcttgtgc cagcggacgc gagcatggtc gggcagtcaa aaacgtcaca
    17281 ccgtcccgtc ctccaagaat ggggtagaag atgaatgtac atgagtttca ggcgaagtcc
    17341 ttgtttgccc agttcggtgt ccccgtcccc cgtggaaagg agatcacgtc gccggaagcg
    17401 gcaacggcct gggccgatga attgaatacc ccggtgttcg tcgtcaaagc ccagattcat
    17461 gccggcgggc ggggaaaagc aggcggcgtg aaaatcacga aggacaaggc ggctgtcgcc
    17521 ggcctggcga aggaactcat cggcaagact ctcatcacgc atcagaccgg accgaaaggc
    17581 cggaaagtgc accgcctgct gatggaggag ggcgccaaca tcgccaaaga gctgtatctg
    17641 tccatcctcg tcgatcgtga cacgggatgg ccgaccttta tcgccagtac ggaaggcggg
    17701 atggaaatcg aagaagtcgc ggagaagacg ccggacaaga tcatcaagga agccatcgat
    17761 cctgccgtcg gttttcaggg gcataacggc cgcaacgtag cctttgcgct cggtttgcag
    17821 aacatcgagc cggcggtcat caacccgttc gtccagatgc tcggcaatct ctatcgcctt
    17881 ttcatggaca agcacgcggc gttggttgag atcaatcctc tcattatcac gaaagaaaag
    17941 acgctggtgg ccctcgatgg caaagtctcg ttcgacgaca acggcctttt caagcacgaa
    18001 gacgtgcaga aaatgcgtga tctgaatgaa gaagagccgt tggaaatcga agccacggcg
    18061 aacaatctca actatgtgaa gttggatggc aacatcggct gcatggtgaa cggcgcaggc
    18121 ctcgctatgg ccacaatgga cgtcatcaag cttgccggca gcgagccggc caactttttg
    18181 gacgtcggcg gcggcgcaac caaagagacg gtggcggccg gattccgtat tttgctcaag
    18241 gacccgaacg tcaaaggcat tttcatcaac atctttggcg gcatcgttcg atgtgagcgg
    18301 atcgctcacg gcgtcattga ggcggccaag gaagtgaaga tcaacgtgcc cttagtcgta
    18361 cgcctgcagg gcacgaacgc cgacgagggc cggaagttgc tggcggaatc aggcttgaaa
    18421 ctggaggttg ccaatgattt gtgggaagcg gcgcaaaaaa tagtgaggtt gacggggaaa
    18481 gcggcctgag cggcctcgcc gtcctgcgct gctccaacgc tacggctcga ccgcactaag
    18541 cctccggttt ctcggccatg caaacttgat ccgttggtcc tgcggcaacg gaccttcgaa
    18601 catgcacagc cgtcgcttcg cgaccgcaac cgttcggcaa gtgcggttcc cgcgccttcg
    18661 ctcaagtcgc gacgcaggac cggcaaggtc gtttgtgatg gaagccctgc tccgtcaaga
    18721 gagggaaaga ggaaatcaag aagagaggtc aatcgtgagc attctcgtca ataaaaatac
    18781 gcgggtggtg gtgcagggca ttaccggcaa ggaagggtcg tttcatgcga ctcagtgcaa
    18841 ggcgtatgga acgaaggtcg tggctggagt cacccccggg aaggcaggcc aagaggtgga
    18901 aggcatcccc gtgttcaaca cggtagctga tgcggtaaag aagacggaga gcgatacctc
    18961 tttaatcttc gtcccaccgc cgttttgtgc cgacgcgatt ttggaagctg ctgatgctgg
    19021 aatcaagctg atcatttgta tcactgaagg catcccggtc aatgagatgg tcagagtcaa
    19081 gcgtgccctg cgcggacggg atgtgcgact gatcggtccg aattgtcctg gcgtcatcac
    19141 cgtcgatgaa gcgaagatcg gcatcatgcc gggattcatc cataaaaaag gcatcgtcgg
    19201 cgtcgtctca cgcagtggca ccctcaccta tgaggcggtg catcaactgt cgaccctggg
    19261 cttgggggaa actacttgtg tggggattgg cggggatcct gtgaatggga caggctttgt
    19321 ggatgtgttg ccgctctttg agaaggatcc tgagacgcag gccatcgtca tgatcgggga
    19381 aattggaggc gatgcggagg aaaaagcggc cgagtacatc aagaagcacg tcaaaaagcc
    19441 ggtagtgagc tttatcgccg gcatcacggc acctcccggc cgccgcatgg gccatgccgg
    19501 ggcgatcatc tcggggggta aaggcacggc aaccgaaaaa atgaaggccc tggaagccgc
    19561 gggagtccgg gtcatcaaga atcccgccga aatcggtatc acggtcaaga aagcgctggg
    19621 acgttagaga attttaaagt gtaggttaat tcaacttccg aggccgtatt tgctttccct
    19681 tcacctccta tcgtggtagg ctctatcctg cagcctcaca agatatgttg tcccgcaggt
    19741 attcctccgg ccgcccaggc ctcagtcttt gagcgaggcc aaaccaggag tctgtttgcg
    19801 agtatttctc gtccacgttc gcgaccccca gttttacgcg cttcccgcta aaacacgggc
    19861 taaaaacggc aacatccgtg tgatgggttt cccgcccatc gggatcatgt cgctatccgc
    19921 cgtgctgaag gaagccggac acgaatgtgt gatgttcgat caggcgaatc cggatacgcc
    19981 caatcatgtc attgttgatg aaatcaagaa gcagaaacct gcgctcatcg gcctgagctt
    20041 cctgagtacg acgagctatc cttatgcaaa gattctcgcc cgccaaattc gtgccgctga
    20101 ttcaacagtc aagctcgcgt ttggcggggt gttcgccagt ctgaatgccg ggctggtcaa
    20161 gctccaatgt ccagaagtcg atttcgtatg tcgcggtgac ggcgagcagc tgttgctcga
    20221 tctgctcact cagatagacg cgccggaaac cgtcggcg
//
LOCUS       DASSCQ010000029         6376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1453400, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000029 DASSCQ010000000
VERSION     DASSCQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453400"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1423)
                     /locus_tag="VFH05_02105"
     CDS             complement(38..1423)
                     /locus_tag="VFH05_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6677036.1"
                     /translation="MVTNTLTLVNHASVLIKGKKQSILTDPWYDGEAFHRGWRLLYEN
                     KENEIEYVLNNTKFIWISHEHPDHFSIGFFRKYKSLIIDNKIVVMFQHTKDKRVISFL
                     KKGGFQSMELASGEPFALEDKFTVRVVKDEFYDSALLVDVAGISIFNLNDCAMHSQKR
                     ITDFKNQYGCCDVLLTQFSYAAWKGGRDHIEWRRSAAQDKLTSLVRQGQTLQAKTVIP
                     FASFVYFANVLNSYLNDGVNTPRRVREFCRASNADFRCVFLKPMESLDLDDNDRPKQQ
                     ESSLSFWENAYASNKTYIQYEESYSIDQLSKLFKTYCERLKKKNAWWLIRVCRYLRIA
                     FRPITVKLVDTGEVLRVDLSKRKLGPAVADPEIALHSESLAFIFKFPYGFDTLGVNGT
                     FEELKQGGFDKFTKTFAIENLNNIGYSFKLSLAFDLNVILIFIGRLMRASRKLNAGKP
                     VAREVVRARAA"
     gene            complement(1566..2801)
                     /locus_tag="VFH05_02110"
     CDS             complement(1566..2801)
                     /locus_tag="VFH05_02110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6677037.1"
                     /translation="MQTAAISPVEEQESVQPHERIGTGPPEEVPVKELSVDVVRDYES
                     FVALKSVWNRLVTEAGIDHPFIRHEWVRTWWECFEHHGTLHILVVREGSEPIAIAPLM
                     LDHGRLFGCPVRRLRGIANVYTERFDIILTRRAEEASKAIWQCLSADSSTWDLLELRQ
                     IPNGARGVEHLRFWAFESNFLLGQWHSSDGPFIPIDRPWEAYAKTLSKKHLSNLRGRT
                     KGLNRIGEVQHEIVTGGEHLKRALDEAFLLEAAAWKGKAGTAIMNRGDRLAFYRTLLT
                     RASEAGWLQLHFLAVSGKRIAVQITLLVQNKLYILKSGYDPHFAPFAPSLLLCELMLR
                     DAWKRGLAEVDFLGNSERWKLEWAHESRPHSWIFIFPNRPPLRLLHRLKFSVLPRLQK
                     YAAYRFLRSGICGLKVNED"
     gene            complement(2910..4031)
                     /locus_tag="VFH05_02115"
     CDS             complement(2910..4031)
                     /locus_tag="VFH05_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6677038.1"
                     /translation="MSGCHLPFPLNAQNTTYFHTARSAIYYLFRELVKSGRGVVLVPD
                     YHMGNEVRAIRASGAQIAWYPATRRFELDLDGLRRSCQRARAQVLFVIHYAGWPQPVE
                     ALRTFCEEYGLILVEDCAMALLTEREGQPVGTSGDYAIFCLYKTLPLPDGAVLVQNRA
                     KFDQLTALSLRRSGRVFTAGRIAELGLERIRSRWPNIGRHLMDWKLAAGRLLTRLRVE
                     RVPVGDTGFDVANVDIGMASVSRRLLTRLDYEAIKKRRREHFHLLAEQFPVEAVRTDL
                     EPGVCPLFFPLLVADKARAAQALWSRGVMATPLWNEGDASLGLHEGEASRFLRRHVLE
                     LPIHQDLNQKHLTYMARQVRDLGIALRSESIREPEFQPV"
     gene            complement(4099..5913)
                     /locus_tag="VFH05_02120"
     CDS             complement(4099..5913)
                     /locus_tag="VFH05_02120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6677039.1"
                     /translation="MQPNSAVSAMEHTDTRRPSTGPLRVEVVTDYRDFLKLEPEWNRL
                     VEEAGIDFPFVRHEWIRADWDCYHHGATLYIIVVHDRGRPLAIAPLMLERVTTYGLPV
                     QRLRGIAHVYTERFDFILSVRPQECCEAIWTHLADEAHQWDVLELPHLPARSLTLDMF
                     PPMTARTRCALTRWPSAESPYVAVRESWDTYCKGLKKTHRHDMRKRVANLEQKGPLEL
                     EVILSDHCMDRDFDDALRLESSAWKGAEGTAIESRKDSISFYRDVIQIAVRNAWLRLY
                     FLKVGGVRIAVRIALLFQNKLYMLKAGYDPQYASYAPGHVLSMKILEEAWRLRFDEVD
                     FLGDAERWKLDWATDLHRHVWLFVFPGHLKSRLLYHLKVRVMPRIKATRRAVLRHGVR
                     GCLTLAARHAKQNVLTTLYRHERHVWYVAAPGEVHAAPLADGLKLRRSRREHLDLLTQ
                     SNLYGWSAAESYLGEGGDLWMVRDGQRPVFCCWVFRKRMPTVAAPGGWKDLPAKTVCF
                     EGVVTDAKYRGRGIAPAAWALIAQSLHDEGIRMIAIKVEEKNRSMRRAVQKAGFHEMA
                     ITDFRQVAGHSRIQVVPVNGTSGRGADILAEVQKLAKK"
     gene            complement(5903..>6376)
                     /locus_tag="VFH05_02125"
     CDS             complement(5903..>6376)
                     /locus_tag="VFH05_02125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6677040.1"
                     /translation="EIAGAGSRERDLHALARSLKLTGVHFLGPVAPSDVPGLHERADI
                     LMNSALIDNQPLSILEAMASGMPVVSTNVGDVGNMIEHGVSGTIVPQKDPAAMAAAVV
                     FLLKYPDAARAMASRAVERLGRYSWNRVGCQWSDLYSDVMQQRPNERPRGIRHAA"
BASE COUNT         1406 a         1909 c         1705 g         1356 t
ORIGIN      
        1 tgagaaggaa tccgccgatg atgaacacag gctccactca cgccgctctg gctctgacga
       61 cttcacgggc caccggcttc cccgcattca atttgcgcga tgcccgcatg agccttccga
      121 tgaaaatcag tatcacgttc agatcgaacg cgagtgataa tttgaaggaa tacccgatgt
      181 tgttcaaatt ttcgatggca aaggtcttgg taaacttatc aaacccgccc tgtttgagtt
      241 cttcaaatgt gccgttcacc cccagcgtgt cgaacccgta aggaaatttg aaaatgaacg
      301 ccaacgattc cgaatgcaac gcaatttccg gatccgcaac ggccggacca agcttgcgct
      361 tcgacagatc cacacggagg acttctcccg tatccacaag cttgaccgtg attggcctga
      421 acgcgatgcg caaataccgg cacacccgaa ttaaccacca cgcattcttc tttttcagtc
      481 tctcgcaata ggtcttgaac aatttagaca gttgatcaat cgagtaggac tcctcatatt
      541 gaatatacgt tttattcgat gcgtacgcat tctcccaaaa cgacagggac gactcctgtt
      601 gtttcggacg gtcgttgtcg tccaaatcga gagattccat cggcttgaga aagacgcatc
      661 gaaaatcggc gttcgacgcg cggcagaatt cccggacgcg tcgaggcgtg ttcacgccat
      721 cattcaagta gctgttcaag acgttggcaa agtacacgaa gcttgcgaag ggaatgacgg
      781 tcttggcttg taacgtttgc ccttgccgga ccaggctcgt caatttatcc tgcgccgccg
      841 agcgccgcca ctcgatatga tcccgcccgc ctttccacgc ggcataactg aactgggtga
      901 gcaatacatc acaacaacca tactggttct tgaaatccgt aatgcgtttt tgggaatgca
      961 tcgcacaatc gttcagattg aaaatgctga tccctgccac atcgacaagt agcgcggagt
     1021 cgtagaattc gtccttgacc acgcgaacgg tgaacttatc ttccagcgcg aacggctctc
     1081 ccgacgcaag ctccatactt tgaaagccgc ctttcttgag aaaactgatg acgcgcttat
     1141 ctttcgtgtg ctggaacatg acgacgatct tgttgtcaat gatcaagctc ttgtactttc
     1201 taaaaaagcc tatggaaaag tgatctggat gctcatgcga aatccaaatg aacttggtgt
     1261 tattcaggac atattcaatc tcattctctt tgttctcata gagcaaccgc catccccgat
     1321 gaaacgcctc gccgtcgtac cagggatccg tcaagatgga ttgcttttta cctttgatca
     1381 gtaccgatgc gtgattgact aacgtcagcg tgttcgtgac cataatcttc tccaacgatc
     1441 aaagagccca ccggctttga gcggtgtctg ttcgtacacg tcgtaccgta aagctgtcat
     1501 ccgaggggtc ggatacgatg accaaacggc caagccaaga gaccgtggct cacatgcgag
     1561 atggtctagt cctcgttgac cttcagtcca caaatcccgc ttcgcagaaa gcgataggcg
     1621 gcatattttt ggagccgcgg taagaccgaa aatttcagtc gatgcaagag acgcaacggc
     1681 gggcgattcg ggaagataaa gatccaggag tgcggcctgc tctcgtgggc ccactcgagc
     1741 ttccagcgtt cagaattgcc gagaaaatcc acctcggcca agccgcgttt ccaggcatcg
     1801 cgcaacatta attcacacag cagcagggag ggcgcgaagg gcgcaaagtg cggatcatac
     1861 cctgatttga ggatgtagag cttgttctga acgagtagag taatttgcac cgcgattcgt
     1921 ttcccactga ccgccaaaaa atgcagttgc agccatcctg cctccgacgc tcgcgtcaag
     1981 agcgtccggt aaaacgcaag gcgatcgccc cgattcatga tcgcggtacc ggctttcccc
     2041 ttccacgcgg ccgcctccaa gaggaaggct tcgtccaacg cacgcttcaa atgttcaccg
     2101 cctgtcacaa tctcgtgctg gacttctccg attcgattca accctttcgt gcgaccccgc
     2161 aggttggata aatgtttttt ggacagagtt ttcgcatagg cctcccatgg ccgatcgatc
     2221 ggaatgaatg gaccgtctga tgaatgccat tgaccaagca gaaaattgct ttcgaatgcc
     2281 cagaagcgaa gatgttccac cccccgcgcg ccgttcggga tttgccgcag ttccaacaga
     2341 tcccatgtgg aggagtcggc agaaaggcac tgccaaatgg ctttggaggc ttcttcggcc
     2401 cgacgggtca gaatgatgtc aaaccgctct gtatagacat tggcgatccc ccgtaagcgg
     2461 cggacgggac acccaaagag acggccgtgg tcgagcatca gcggcgctat ggcgatcggc
     2521 tcgctccctt cccgcacgac cagtatatgt aaggtgccgt ggtgttcgaa gcactcccac
     2581 caggttcgaa cccactcatg ccggatgaaa ggatgatcga tgcccgcctc ggtgacgagt
     2641 cgattccaaa ccgatttcag ggcgacaaag gattcgtaat cccgaaccac gtcgaccgat
     2701 aactctttga ccggcacctc ctcaggcggg ccggtcccga ttcgttcatg tggctgcacc
     2761 gattcctgct cctccactgg acttatcgct gccgtttgca tgccacttcc ctcctttttg
     2821 ctccgggcac ggcccgaaga cgctggcata gaatgggtca tccgtagttt gagtggatcc
     2881 ataatacgtg tgtaccgtca tcacctggat cacaccggct ggaattccgg ctcgcgaata
     2941 gattcggagc gaagcgcgat gccgagatcc ctcacctgtc gtgccatgta tgtcaggtgt
     3001 ttctgattca ggtcctgatg aatcggcagt tccaacacat gacggcgaag aaagcgtgac
     3061 gcctcccctt catgcaatcc gagggacgca tcaccctcat tccagagtgg cgtcgccatg
     3121 acgccacggc tccaaagcgc ctgtgccgct cttgccttgt ccgccacgag caatggaaag
     3181 aacaacggac agactcccgg ctccagatcc gtgcggaccg cttctacagg gaactgctca
     3241 gccaagagat gaaaatgttc gcgccgccgt ttcttgatgg cttcatagtc cagccgggtc
     3301 agcaacctcc gcgagacaga cgccatcccg atatcgacat tcgcgacatc gaatccggta
     3361 tctccgaccg gaacccgttc aacacggagc cgggtcagca atcggccggc agcgagtttc
     3421 caatccatca agtgccgccc tatgttcggc cagcggctgc gaatccgttc caaaccgagt
     3481 tcagcgatgc ggccggcggt gaagactcgg ccgcttctcc gcaacgacag ggctgtcaac
     3541 tggtcgaatt tcgcccgatt ttggacgagg acggctccat cgggaagtgg aagcgtcttg
     3601 tagagacaga aaatcgcata gtcgccggag gtgccgaccg gctgcccctc tcgctctgtg
     3661 agcagggcca tcgcgcaatc ctccaccaat atcaagccgt actcttcgca gaacgttctc
     3721 agggcttcga ccggttgcgg ccaaccggca tagtgaatga caaacagtac ttgggcacgc
     3781 gcgcgctgac aggaccggcg cagcccgtcc aagtcaagct cgaaccgtct tgtcgccgga
     3841 taccaggcga tctgcgctcc ggacgcacga atcgcccgca cttcgttgcc catgtggtag
     3901 tcgggcacca gcaccacgcc acggccgctc tttacgagtt ccctgaacag gtagtagatg
     3961 gcgctccggg ctgtgtgaaa ataggtcgtg ttttgcgcgt tgagagggaa cggaagatgg
     4021 cagccactca tgccccgcca ctccttcatt cccagtcctg gccatgcggg cagatacatt
     4081 gctctgcgcc tccttccatc acttctttgc aagcttttga acttcggcca ggatgtccgc
     4141 cccgcgaccg gacgtcccgt tgaccggcac cacctggatg cgagagtgtc ccgccacctg
     4201 acggaagtcg gtaatcgcca tttcgtgaaa cccggccttt tggaccgccc gtcgcatgga
     4261 ccggtttttt tcttctactt tgatggcgat catgcgtatc ccttcgtcgt gcagactctg
     4321 agcgatcaag gcccaggccg ccggcgcgat cccgcggccg cggtatttgg catccgtcac
     4381 cacgccttca aaacagacgg tcttagcggg caggtccttc cagccacctg gtgcggcgac
     4441 cgtcggcatg cgtttccgaa agacccagca acaaaacacc ggccgctggc catcgcgtac
     4501 catccacagg tcaccgcctt ctccgagata cgattccgct gcgctccagc catacagatt
     4561 ggattgagtc agcagatcaa gatgttcacg acgggaccgg cgtaacttca gtccgtccgc
     4621 cagaggcgcg gcgtgaactt cgcccggtgc ggctacatac cagacatgcc gctcatgtcg
     4681 atataaggtc gtgagcacat tttgcttcgc atgacgtgcg gccagcgtga gacagccacg
     4741 gacgccgtgt cgcagtactg ccctcctggt cgccttgatg cgcggcatca cacggacctt
     4801 cagatggtat aagagccggg atttgagatg gccggggaac acaaacagcc agacatgccg
     4861 gtgcaggtcg gttgcccaat cgagcttcca tcgttctgcg tcgccgagaa agtccacttc
     4921 gtcgaagcgt aatcgccagg cttcttccag gatcttcatg gacaagacat gtcccggggc
     4981 ataggaagca tattgcggat cgtatccggc tttcagcatg tagagtttgt tttgaaacag
     5041 cagcgcgatc cgcaccgcga tgcgaacacc gcccaccttc aaaaaataca accgaagcca
     5101 agcgttgcgc accgctatct gtatgacatc acgataaaag gagatgctgt ccttccggct
     5161 ctcgatggcc gttccttccg cgcccttcca ggccgaagat tccaatcgca gcgcgtcgtc
     5221 gaaatcgcgg tccatgcagt gatccgacag gatgacttcc agttccaggg gacccttctg
     5281 ttccaggttc gccacccgct ttctcatgtc gtgccggtgc gtttttttaa ggcctttgca
     5341 ataggtatcc catgactctc ggaccgccac gtagggagac tcggccgatg gccaacgagt
     5401 gagagcacac cgcgttcgcg ctgtcatcgg aggaaacatg tcaagcgtaa ggcttctggc
     5461 cggtaagtgc ggcagttcca gcacatccca ctgatgggcc tcgtcggcga gatgcgtcca
     5521 aatagcttca cagcactctt ggggccggac tgaaagaatg aaatcgaacc gctcggtgta
     5581 gacgtgcgcg atccctcgca atcgctgtac cggcaatccg tacgttgtga cgcgttccag
     5641 catcagcgga gcaatcgcca aaggacggcc cctgtcatgc acgacgatga tatagagtgt
     5701 ggccccatgg tggtagcaat cccagtcggc ccggatccac tcatgccgga caaacgggaa
     5761 atcgatgccg gcctcctcca cgagccgatt ccactcaggt tcaagcttca gaaagtcgcg
     5821 ataatccgtc accacctcga cgcgcagcgg accggtcgac ggtcgccgag tgtccgtgtg
     5881 ttccatggcc gaaacggcgg agttaggctg catgccggat tccacgaggg cgctcgttcg
     5941 ggcgctgttg catcacatcc gagtacaggt ccgaccactg acatccgacc cggttccagg
     6001 aatagcgacc gagccgctcg acggcgcggg aagccatcgc gcgcgccgcg tcggggtact
     6061 tgagcaaaaa gaccacggct gccgccatcg cggcgggatc cttctggggc acaatggttc
     6121 cgctgactcc gtgttcgatc atattgccga cgtcgccgac attggtgctg acgacgggca
     6181 tgcctgacgc catggcttcc aagatcgaga gcggctgatt gtcgatgaga gcggagttca
     6241 tcaaaatgtc agcgcgttcg tgcagacccg gtacgtccga gggagcgacc gggccgagga
     6301 agtgcacacc cgtgagcttc agcgagcggg ccagggcgtg aaggtctctt tcgcgacttc
     6361 ctgcaccggc gatctc
//
LOCUS       DASSCQ010000030         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1071269, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000030 DASSCQ010000000
VERSION     DASSCQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071269"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1275
                     /locus_tag="VFH05_02130"
     CDS             151..1275
                     /locus_tag="VFH05_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6677041.1"
                     /translation="MRIAQVSPLWESVPPRLYGGTERVVSYLTEELVRQGHEVTLYAS
                     GDSQTSARLEAACPHALRLSTGLVNRDAPLILMQEHAMGADADQFDIIHSHLDFLSFP
                     MSRRCPTPVLTTLHGRLDLPELGPIFREYADMQLVSISDAQRLPLPHANWQATVYHGL
                     PDLYTFHPNTGSYLAYLGRICPEKRPDHAIEIAKRVGIPLRIAAKVDPVDREYFVTKI
                     APLMDHPLIEYLGEISDAEKGHFLGDAAAVLCTYDWPEPFGIVLIEALACGTPVFAYR
                     RGSIPEIIEDGVTGFICDSLDEMVAKIDRLPLIKRQRCRESFQARFTAERMVKDYLAV
                     YERMATVPARAHAFANGRSLRMVGPRPVAVSELTTAHGTA"
     gene            1417..2061
                     /locus_tag="VFH05_02135"
     CDS             1417..2061
                     /locus_tag="VFH05_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677042.1"
                     /translation="MGVRRDDKRLLSVVLIAILWLPTACRPVPVADHSHTTMLLDDVA
                     VPQHVRRLAVWYPRTWEQELSYGYSRLEQAAFQLKKRRADIKIVERRNLEQLTDEQRF
                     QLSGRVGDDSVVRVGRWLGADSLVLFRIDGPTWRERLLARMHGKMPPFVVSSKIISLE
                     SGEVLYHDVVTAAPVPDSGEWSDYDSDHQLRPAMHAALDQALSTAILHLHQSFR"
     gene            complement(2078..2548)
                     /locus_tag="VFH05_02140"
     CDS             complement(2078..2548)
                     /locus_tag="VFH05_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677043.1"
                     /translation="MSVPAASPLLPSTTERVPQQTDPAINERITQQTEANVEAYSADR
                     RLIDVRLEELDGEWDIERILEANAAAVSLIGLTLARFVSRRWFLLPAAVAAFLLQHAV
                     QGWCPPVPLFRRLGIRTQREIDEERYALKAMRGDFSAANAGSSSARDVLRAVRS"
     gene            complement(2644..3153)
                     /locus_tag="VFH05_02145"
     CDS             complement(2644..3153)
                     /locus_tag="VFH05_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677044.1"
                     /translation="MDILSHGLWGGIAFGRKSRRSFWLAFAIGLAPDLLSFGILWTAV
                     ILGLADPPDFSHGTPPESTIPPYVHHLYDVTHSFVVFLFVFLLIWFATKRPRWEIAAW
                     GLHVLVDVPTHSHAFFATPVLWPLVGWKFDGWQWMTPAILIPNYLLLALLYAWFLSLS
                     VRTVPGRPD"
     gene            3351..4697
                     /locus_tag="VFH05_02150"
     CDS             3351..4697
                     /locus_tag="VFH05_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677045.1"
                     /translation="MARLGSGPRDVVKAVFLIMLLIVLPLTGVILAGKPIDQYLEFPP
                     HTRYVEHPPFSRHTFAILGLVTALSLGPPIVRVVTSRVTAHGNVGRAKAYPWWGVVGG
                     ALTAVSWWLAWTRFDWFTSYQASTFTPLWLGYILFVNAWTYTRTGGCMMLGQPRMFLW
                     LFPVSAVFWWSFEYLNRFVQNWYYVGAQTLTSWDYFMQATVPFSTVLPAVLSTRELLS
                     TFPRLSAGMDRLPAFHVRHLHMAGWGVFILACAGLMSLGIWPNYAYPLVWLAPLALIT
                     SLQMVTGEETIFSATPRGDWRSLWWAALAGVVCGICWELWNYKSLAHWEYAIPFVHEY
                     KIFEMPILGYAGYLPFGLECLAVTKFLFPDHDRERMTPMTDSSSHVNDQRVTDAGSAM
                     SASVSRGGVLFQRMSLSGQLEREFLAFGLKRFLVRFKRDVTGWVVARYLDSALSWH"
     gene            complement(4667..>4903)
                     /locus_tag="VFH05_02155"
     CDS             complement(4667..>4903)
                     /locus_tag="VFH05_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677046.1"
                     /translation="LSAPLGWADEDKTTPRKGSVRVEDLPKPIPELLQKIQSLSQKIE
                     PEITRLGSKLGEELTTTVKKLCDELQCQDKAESK"
BASE COUNT          997 a         1374 c         1442 g         1090 t
ORIGIN      
        1 tagccttcgg aaaacccctt gcttagggcc atcttgcgca ggtcggctaa gcggcatgtg
       61 gcgtgcaatc cttcaccacc agctagcttc cagtgccttg acgatttatg aagagtgaat
      121 taataagaga cacaagaaag gaagatcgac atgagaatcg cacaagtctc gcctctgtgg
      181 gaaagcgtgc caccgaggct ctacggcggc accgagcgcg tggtctcgta tctgacggag
      241 gaactcgttc gccagggcca cgaggtgacg ctgtatgcca gcggggattc ccagacgtca
      301 gcccgccttg aagcggcttg tccacacgcc ctgcgtctga gcaccggtct tgtcaatcgt
      361 gacgcgccgt tgattctgat gcaggagcat gcaatgggcg ccgacgccga tcaatttgac
      421 atcatccatt cgcatctgga ctttctgagt tttcccatga gccgtcgttg tccaaccccc
      481 gtgctcacaa ctttgcatgg acgcttggac ctgccggaac tgggtccgat ctttcgagag
      541 tatgcagaca tgcagttggt gtccatttcg gatgcccagc gtcttccgct tcctcatgcc
      601 aattggcagg cgaccgtgta tcacgggttg cccgatctct atacatttca tcccaacacg
      661 ggatcctatc tggcatacct cgggcgtatt tgtcccgaaa agcgccccga tcatgcgatt
      721 gaaattgcca aacgggtggg gattccgctg cgaatcgcgg cgaaagtgga tccggtcgat
      781 cgggaatact ttgtgacgaa gattgcgccg ttgatggatc atcccttgat cgaatacctc
      841 ggcgaaatca gcgatgcgga gaaagggcat ttcctcggtg atgccgctgc agtgctgtgc
      901 acatacgact ggcccgagcc cttcgggatc gtcctgatcg aagcgctggc ttgtggcacc
      961 ccggtgttcg cctaccgccg cggctctatt ccagaaatca tcgaagacgg tgtgaccgga
     1021 ttcatctgcg acagcttgga cgaaatggtg gcgaagattg accgcctacc gctcattaaa
     1081 cggcagcggt gtcgtgagtc gtttcaagcc cgattcaccg ccgagcgaat ggtcaaagat
     1141 tatctggccg tgtatgaacg gatggcgacg gtgccggcga gagcgcacgc gttcgccaac
     1201 ggacggtccc ttcgcatggt cggtcccagg cctgtggccg tcagtgagct caccaccgcg
     1261 cacggcacag cctaagcacc agaaaaggcc gggtccaacc atgttggacc ccggcctttt
     1321 cattaccctt cctcccactc atctcttgct atcgtaggca ggctccaccc ttccgtagga
     1381 tagcgggctt cctgcccggc ctcctactat ggcgttatgg gtgtccgcag agacgacaag
     1441 cgattacttt ctgtcgttct aatcgcgatc ctttggctgc ccacagcctg tcggccggtt
     1501 ccggtcgctg accactccca cacgacgatg ctcttggacg atgtcgcagt tcctcaacat
     1561 gtccggcgcc ttgccgtctg gtatccacgc acctgggaac aggaactttc ctatgggtat
     1621 agcaggctcg agcaggccgc ctttcagctc aagaagcggc gggcggatat caaaattgtg
     1681 gagcggcgca acctggaaca gctcacggat gaacagcgtt ttcaactatc cgggcgggtc
     1741 ggcgacgaca gcgtcgtgcg agtgggcaga tggttgggcg cagacagcct cgtcctcttc
     1801 cgcatcgacg ggcccacatg gcgggaacga ttgttggctc gcatgcatgg aaagatgccg
     1861 cccttcgtcg tttcgagcaa gattatctca ctggaaagcg gagaagtgct ctaccatgat
     1921 gtggtgacgg ccgctccggt cccggacagc ggcgagtgga gcgattacga cagcgatcat
     1981 caactgcgtc ccgcaatgca tgccgcactc gaccaggccc tgtcgacagc cattctgcat
     2041 cttcaccagt catttcgtta aggctccgcc gctcatctca gctgcgaacg gctcgcagca
     2101 catcccgtgc ggaagacgat cctgcgttgg cggccgagaa gtctcctcgc atcgctttca
     2161 acgcatagcg ttcctcgtcg atctcccgct gggtgcgaat cccgagacga cggaacaagg
     2221 gcacgggcgg acaccatccc tgcacggcgt gctggagcaa aaaggcggca acagcagcgg
     2281 gaagtaagaa ccaccgtcgg ctcacaaagc gtgcaagcgt caatccgatc aaagagacgg
     2341 ccgcggcgtt ggcttcgaga atccgctcga tgtcccactc tccgtccagt tcctccagcc
     2401 tcacatcaat caggcgtcga tcagcggagt atgcttccac atttgcctcg gtttgctgcg
     2461 tgatacgctc gttgatcgcg gggtcggtct gttgaggaac ccgctccgtc gtggaaggga
     2521 gaagtggaga cgcggctggg acggacatgg aggcctccat caaggtgcag ttgaggagat
     2581 agaattgccc attctgaacg agactgcacc tcggagcttc cccagcgtga ggtgacagac
     2641 gcgctaatcg ggccggccgg gaacggtacg aacagacagg gataggaacc acgcgtaaag
     2701 cagcgcgagc aagagatagt tgggtatgag aatggctggc gtcatccatt gccatccgtc
     2761 aaacttccaa cccaccaagg gccagagaac cggggttgcg aagaaggcgt gtgaatgggt
     2821 cggcacgtcg accagcacgt gcagacccca tgcggcgatc tcccagcgcg gccgctttgt
     2881 cgcgaaccag atgagaagaa agacgaagag aaagacgacg aagctatgcg tgacgtcgta
     2941 caggtgatgc acatagggtg ggatcgttga ttccggcggc gtaccgtggc tgaaatcggg
     3001 tggatctgcc aaaccgagga taacggctgt ccacaggatt ccgaaagaca acaaatccgg
     3061 agcgagcccg atcgcgaagg cgagccaaaa gctacggcgg ctctttcggc caaaggcaat
     3121 gccgccccat aatccatgtg aaagtatgtc cataatggct tcgagtagac gaaccgcgat
     3181 accataacaa attttccact gacttcccgc agagtcggac taggaaggct cccagctagt
     3241 tacgcgatca ttcattgcca caattgcggg attgtggagc agagcccacg cctgacgagc
     3301 gaagttcgat gtcaacgtac agcacagttc ggactgaagc cggagaaaat atggctcgat
     3361 tgggcagtgg cccacgtgat gtcgtgaaag ctgtgttcct gatcatgctg ttgattgtat
     3421 tgccgttgac gggcgtgatc ctggccggca aaccgatcga tcagtatctc gagtttcccc
     3481 cgcacacccg gtatgttgaa catccgccgt tctcccggca cacgttcgcc atcctgggac
     3541 tcgtcaccgc gctgtcgctc gggccgccga ttgtccgcgt cgtgacctcg cgagtgactg
     3601 cccatggcaa cgtcgggagg gcaaaagcct atccctggtg gggagtcgtc ggcggcgcgc
     3661 tcacggccgt ctcctggtgg ctagcctgga ctcgcttcga ctggttcaca tcctaccaag
     3721 cctccacctt cacgcctttg tggctggggt acattctgtt cgtgaatgcg tggacgtaca
     3781 cccgcacggg aggctgcatg atgttggggc agccgcgaat gtttttgtgg ctgtttccgg
     3841 tgagtgcggt gttctggtgg tcattcgaat atctgaaccg cttcgtgcag aattggtatt
     3901 acgtcggcgc acagacgctg acgtcatggg actatttcat gcaggccacg gtgccgtttt
     3961 cgaccgtcct tccggcggtg ctgagcacca gagaattatt gagtacgttt ccccgactga
     4021 gcgcagggat ggaccggctt ccggcgtttc acgttcggca cctgcacatg gccgggtggg
     4081 gcgtgtttat tctggcttgt gccggactga tgagcttagg gatttggccc aactacgctt
     4141 atcccctcgt gtggctcgcg ccactcgcgc tgatcacgtc gcttcagatg gtgaccggtg
     4201 aggaaacgat cttttcagcg acgccgcggg gtgattggag atcattgtgg tgggcggcgt
     4261 tggcgggcgt agtctgtgga atctgctggg aactgtggaa ctacaagagc ctggcgcatt
     4321 gggaatacgc cattcccttc gtgcatgagt acaaaatttt tgaaatgccg attcttgggt
     4381 atgctggtta tcttccattc ggcttggaat gtctggctgt gacgaagttt ctgttccccg
     4441 atcatgatcg ggaaaggatg acgccgatga ccgattcatc gtcccacgta aacgatcaac
     4501 gtgtcacaga tgctgggtcg gcgatgtcag cctcagtttc acgaggaggt gtgctatttc
     4561 aacgtatgag tctttcgggc cagctcgagc gcgagtttct tgcctttggc ttgaaacggt
     4621 tcctagtcag gtttaagcgt gatgtgaccg ggtgggtagt tgctcgttac ttggactcag
     4681 ccttatcttg gcactgaagt tcatcgcaga gctttttcac tgtggtggtg agctcttctc
     4741 cgagtttgct gcctagccgg gtaatttctg gctcgatctt ctggctcaac gattgtattt
     4801 tctgcaagag ctcaggaatg ggtttgggca gatcctcgac ccgaacagat ccttttcgag
     4861 gtgttgtttt gtcttcatca gcccacccca atggcgctga aag
//
LOCUS       DASSCQ010000031        10432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_154299, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000031 DASSCQ010000000
VERSION     DASSCQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154299"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1572
                     /gene="ffh"
                     /locus_tag="VFH05_02160"
     CDS             226..1572
                     /gene="ffh"
                     /locus_tag="VFH05_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET6677047.1"
                     /translation="MLDQLSDKFEKILKKLRGTGVLTEENIAEALKEVRLALLEADVN
                     YKIVKDFVERVRQKAIGQEVLHSLTPGHHVVKVVWDELRAIMGQERAGLSLASKPPTV
                     VMMVGLQGSGKTTTCGKLAKLFKQQGKRVLLVAADPRRPAAGDQLASIGRDLEVDLHR
                     LDHPGTAQDDVVRICRAGVERGREQGFDLVVLDTGGRLHVDDELMNELVAVKSAVQPN
                     EVLLVADAMTGQDAVTMATQFDQRVGLSGIILTKVEGDARGGALLSIRAVTGKPVKFL
                     GVGEKLDALELFHPDRMASRILGMGDVLTLIEKAQETFTREQAEEAQQRLTSDTFTLE
                     DFRSQLGQVNKLGSLEQILGMLPGGQKLKDAMAGQVPERELKRVMAMIDSMTLRERRD
                     HTVINGSRKKRIARGSGTNVTEVNRLIKQFLSARKIAKAMTGTGGRRQLAQLLRSM"
     gene            1676..1960
                     /gene="rpsP"
                     /locus_tag="VFH05_02165"
     CDS             1676..1960
                     /gene="rpsP"
                     /locus_tag="VFH05_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET6677048.1"
                     /translation="MAVHLRLTRTGRHKRPIYRVVAADSRKPRDGRFLEILGIFDPLK
                     EAGLPELKQERVLSWLHQGAQPTVTVKTLLRRSGVWKQFETEKAAKKNKA"
     gene            1963..2487
                     /gene="rimM"
                     /locus_tag="VFH05_02170"
     CDS             1963..2487
                     /gene="rimM"
                     /locus_tag="VFH05_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET6677049.1"
                     /translation="MTAFLPDMVTIGKVERPFGVHGAVKVRSLSDRPGRFEQLHEVRM
                     AGERGQTIDRRVRSVRKAGPVYIVEFEGITTPEEARGIQGAMLQVPRGEASASPDEPL
                     YECDLIGMTVVDETGRDLGRLDTIWELPGHQVLVVKQADREVLIPAVKEFVTGVDVAQ
                     GRMTVRTLEGMVEV"
     gene            2488..3270
                     /gene="trmD"
                     /locus_tag="VFH05_02175"
     CDS             2488..3270
                     /gene="trmD"
                     /locus_tag="VFH05_02175"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET6677050.1"
                     /translation="MRCDVITLFPEMVTPVLESSMLKRAQEKGLLEVKVHNLRDYTLD
                     KHKVADDLPYGGGAGMVMKAEPILRAVEQIRQPYEQRGEKVRVLLPSPQGRPFTQAYA
                     QGLAADSRPMLIVCGHYEGIDERVRIALKPEEVSVGDYVLTGGELPALVIIDAAARLI
                     PGVLGAPESVVEESFSEPLLEYPHYTRPGEVRGITVPEILLSGHHEAIRLWRRKEALR
                     TTYERRPDLLKDRRLTAEDQQLLDDIIREGVRGTPVPCGEEG"
     gene            3275..3703
                     /gene="rplS"
                     /locus_tag="VFH05_02180"
     CDS             3275..3703
                     /gene="rplS"
                     /locus_tag="VFH05_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET6677051.1"
                     /translation="MNQLERIQRSLQKKVVPRFEIGDTVRVHVKVVEGEKERIQVYEG
                     AVIARKGSLNTEMFTVRKVSYGVGVERIFPVHSPIVTKIDVVRQGKVRRAKLYYLRGK
                     KGKFSRVEDREFVGESKASAGSPRVTEPAEEPVEAAAAKS"
     gene            3740..4423
                     /locus_tag="VFH05_02185"
     CDS             3740..4423
                     /locus_tag="VFH05_02185"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET6677052.1"
                     /translation="MTARILNFVGPTNEFELEARRCGYRRVAGLDEAGRGPLAGPVVA
                     SAVVLPSRCRLIGCDDSKLLSESEREQLYVVIRKRAVSIGIGCATEQEIDRLNILEAT
                     RLAMHRALAGLMPKPDCLLIDAISLPACPIPVRSIIKGDALCMSVAAASIVAKVTRDR
                     LMAEYHRVYPHYNFLSHKGYGTEEHLLQLATHGPCQIHRRTYAPVADVIARSRDVLPQ
                     HVQQELDGQ"
     gene            4425..4817
                     /locus_tag="VFH05_02190"
     CDS             4425..4817
                     /locus_tag="VFH05_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET6677053.1"
                     /translation="MTILDPRRLFGQEGEETAERYLLQKGYRIVAKNLRTSLGELDLV
                     AEDGDVLVFVEVKARRTTDFGGAVHAVHRQKQTQLVRLAAQFLARHHWSNRPCRFDVV
                     LLQSSQTAGVQVDHIQNAFEVPGDDLRW"
     gene            4825..5493
                     /gene="ftsE"
                     /locus_tag="VFH05_02195"
     CDS             4825..5493
                     /gene="ftsE"
                     /locus_tag="VFH05_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET6677054.1"
                     /translation="MIQLVHVTKQYDRRPALCDVTLEIPKGEFVLLMGPSGAGKSTLL
                     RMLIGAERPDAGQVFVNGKNVTTLKGSEIPYHRRKVGTVFQDFRLLEKKSVFDNVALP
                     LLVQGISRTDIKRKVTEALRAVGVEHKRDQLPMSLSTGEQQRVCIARAIVNGPVVLLA
                     DEPTGNLDPALTGEIIDLFKLINARGTTVVVATHDPSVMTQVNRRVVTLVRGSLMPEQ
                     RVGV"
     gene            5490..6389
                     /gene="ftsX"
                     /locus_tag="VFH05_02200"
     CDS             5490..6389
                     /gene="ftsX"
                     /locus_tag="VFH05_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET6677055.1"
                     /translation="MRTLSYLLREAWANIWTNRTTTVVAIFTTAFTLACVGVFLLLYV
                     NLRAAAGWLQDDIKVMVYLSEGIAGSTVSDIEQQLRADRAVGALHFISKEQALGEFRA
                     QFPSDSHLLEGLGQNPLPASFVVTLAPAFRSPDAVRRWAERVAAVAGVAKVDYNQDWL
                     DALSTVIRSIEVVAIGIGLILSAAAVTIIANTIRLTLFARRDEIAILRLIGATKTFIR
                     IPYLLEGAVLGALGSTCSLLMLKALYELFRQQMRTTGRLRGLEQLTVFFPLSICLVLV
                     AIGIILGCAGSFLSLRRFDEAKV"
     gene            6386..7567
                     /locus_tag="VFH05_02205"
     CDS             6386..7567
                     /locus_tag="VFH05_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET6677056.1"
                     /translation="MRRVGPPATALCLLIAVCLVPPAVWADSINEKIDKERKTLEQLK
                     DQIEEKRRQADEASKKRESILQGIQTLDERHMRYRQSRQDISRKLRKKDQEIETINAQ
                     IARLREGVQVRQQAILARLRVQYMEGRFGHWKPLLASDSYGDFQRRLRYLSAVSERDY
                     SLIETFKSDMATMQEAERQREAARVGMLAYKRSTEKHLEEINAVKKEKKLYLTKLTQE
                     KESYERALQELERSASRIDSLLRELEQRRRAAAAKPPAGHLQRGVKGGLPWPAEGSVL
                     TYFGRQKHPTFNTYVQRKGIEIKTLEGSAIHAVMPGTVVYADWLKGYGLVIILDHANG
                     FFSLYAHASKILTSVGAQVASGQAIGETGDTGMTGENTLYFELREGAEAIDPLQWLAR
                     R"
     gene            7601..8968
                     /locus_tag="VFH05_02210"
     CDS             7601..8968
                     /locus_tag="VFH05_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6677057.1"
                     /translation="MMEHNQRRRLWMLGPMVVVALLSGVLIGKGWERTGHASETYEEL
                     RTFSEVLTQVQKNYVDDTKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEF
                     GGVGIQIGVKENRLAVIAPIDGTPAQRAGIKAGDYITKVNEESTKDLSLMDAVQKMRG
                     PKGTKVNLTIQRDGTSDPLQFTLVRDTIKIESVKSKVMDNIGYVKLTQFQEATGRDLG
                     RVLKQFKDQKVQSTILDLRNNPGGLLTAAVEVSEQFLPNGKLVVYTKGRESKKDEWFA
                     KGRDQMDDSPMIILVNEGSASASEIVAGALQDYGRAVIVGTTSFGKGSVQTILPLGDG
                     SGLRLTTAKYYTPKGRSIQSTGITPDIVIKPQPATTVAKAETKPGDKEPDNKTSKSPI
                     TGGPGKEQSAAPPKDDAVQRNGATPGAAPLDLSGEPSLDQDVQLQKAVELLKTWKIFK
                     ELRPS"
     gene            complement(8969..9442)
                     /locus_tag="VFH05_02215"
     CDS             complement(8969..9442)
                     /locus_tag="VFH05_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HET6677058.1"
                     /translation="MPIPQHNVQLAGLFRSIAELLAAQRANPYRVRAYRRGADALLAL
                     DEDIAEVAQRQALEEIEGIGRDLSEKIQEFLRTGKIALLETLRIPLPEHIREWAGLPG
                     LSESLVAYLHSRLNIGTLADLEQLVRSRMLRTVPGFSGSDEDLLTAIEDLRRRRP"
     gene            9551..9808
                     /gene="thiS"
                     /locus_tag="VFH05_02220"
     CDS             9551..9808
                     /gene="thiS"
                     /locus_tag="VFH05_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET6677059.1"
                     /translation="MAEPLQIQVNGESRDCPSGATVGDLLRELDVKTERVAVELNLEI
                     LDRREFDRRGLKAGDRVEILSFIGGGGPDDGWIRRVPTHAQ"
     gene            9798..>10432
                     /locus_tag="VFH05_02225"
     CDS             9798..>10432
                     /locus_tag="VFH05_02225"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET6677060.1"
                     /translation="MHNDRLVIAGREFKSRLWVGTGKYKDFAETKKAIEVSGADVVTV
                     AVRRVNITDRSKENLLDFIDPKKYTILPNTAGCYTVEDAVRYARLARAAGVSDLVKLE
                     VLGDEKTLFPDTGGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAAPI
                     GSGLGIRNPYNLKIIMETVKVPIVVDAGVGTASDAALAMEYGADAVLMNTA"
BASE COUNT         2301 a         2795 c         3285 g         2051 t
ORIGIN      
        1 gcgattttct cacgtcgacg gcctcttaaa ctattgactg cattcgcgga attctcgaag
       61 tcgaccatag cgaaaggtcc ccgaggtgtc aaccacaatg ccgttctctg cgccttgact
      121 tctccggggg cctccattag actgcccggt gctcttttgc tagatttttc gaggcgatcg
      181 aagaactact cttaaagctt cgattgcctt tttctttggt gtgcgatgct ggatcaactg
      241 agcgacaagt tcgaaaaaat cctgaaaaaa cttcgtggaa cgggtgtcct gaccgaagag
      301 aacattgcgg aagccctcaa agaagtgcgg ctcgccctcc tggaagcgga cgttaattac
      361 aaaatcgtca aagacttcgt cgagcgggtg cgtcaaaagg cgattggcca ggaagtcctg
      421 catagtctga cgcctggtca ccacgtcgtg aaagtcgtgt gggacgaact ccgggccatc
      481 atggggcagg aacgggctgg cctctctctg gcctcaaagc cgcctacagt ggtgatgatg
      541 gtcgggcttc agggatcagg caagaccacg acatgcggca aactcgccaa actctttaag
      601 caacagggca agcgggtgtt gctcgtggcg gccgacccgc gccgtccagc ggccggagac
      661 cagttggcca gtatcggacg ggatttggag gtcgacctcc atcgacttga ccaccctggg
      721 acggcgcagg acgacgtagt ccgcatttgc cgtgccggcg tcgagcgtgg acgggaacag
      781 gggtttgacc tggtggtgct cgataccggc ggccgcctgc atgtggatga cgaactcatg
      841 aatgagctcg ttgccgtgaa atctgcggtg cagcccaatg aagtcttgtt ggttgcggat
      901 gcaatgaccg ggcaggatgc cgtcacgatg gccacccagt tcgatcagcg cgtgggtttg
      961 agcgggatca ttctcacaaa agtggaaggc gatgcgcgcg gcggggcgct gctttcgatt
     1021 cgggcggtga cgggaaagcc cgtcaaattc ctcggcgtcg gcgaaaagct ggatgcgctg
     1081 gaactgttcc accccgaccg gatggcgtcg cgtatcttgg gaatgggcga tgtcctgact
     1141 ctcatcgaaa aggcgcaaga gaccttcacc cgcgaacaag ccgaagaagc acagcagcgg
     1201 ctcaccagcg acacctttac gctagaggat ttccgctcac aactgggaca ggtgaacaaa
     1261 ctgggctcgc ttgagcagat cctggggatg ctgccgggtg gtcagaagct gaaagacgcg
     1321 atggcgggac aggtgcctga acgggagttg aaacgggtca tggccatgat cgattccatg
     1381 acgctgcgcg aacggcgtga ccataccgtc atcaatggga gccgcaaaaa gaggatcgcc
     1441 cgcggaagcg gaaccaatgt gacggaagtg aatcggctga tcaagcagtt tctctccgcg
     1501 cgaaaaattg caaaggcgat gacagggacg ggagggcgcc gccagttggc gcagttgctc
     1561 cgttctatgt gacaggcgta cggaagcgtc ggcaacgcgc cggtggcttc gtggaagagg
     1621 ggaatacggg gcacggctgt cgccggatgc tctctaacgt cgaaggagga agacagtggc
     1681 cgttcatttg cgattgacca gaaccggacg acataaacga ccgatctatc gcgtcgtggc
     1741 ggcggattcc cgcaagccac gcgacggtcg gttcttggaa attttgggca tcttcgatcc
     1801 gttgaaagaa gccggacttc ccgagttgaa acaggagcgg gtgctttcct ggttgcatca
     1861 gggggctcag cccaccgtga ccgtcaagac actgcttcga cgctcaggag tctggaagca
     1921 gttcgaaacg gaaaaggccg ctaagaagaa caaggcctag gtatgactgc cttcttgcct
     1981 gacatggtaa cgatcggaaa agtcgaacga cccttcggtg tccacggggc ggtcaaggtg
     2041 cggtcgctga gcgaccggcc gggccggttc gagcagctgc acgaggtccg gatggccgga
     2101 gaacgagggc agacaatcga tcggcgggtt cgatcggtgc gcaaagctgg tccggtctac
     2161 atcgtcgaat ttgagggcat caccacgccg gaagaagcac gggggatcca gggggcgatg
     2221 ctgcaggtgc ctcgtggcga agcatccgct tccccagacg agcccttgta cgaatgtgac
     2281 ctcatcggga tgacggtggt cgacgagaca ggccgtgatc tcggccgact tgacacaatt
     2341 tgggagttac cgggccacca ggtcttggtc gtcaagcagg cggaccgtga agtgttgatt
     2401 ccggccgtga aagaatttgt cacaggtgtg gatgttgccc aagggcggat gaccgttcgc
     2461 acccttgaag gaatggttga ggtctgaatg cgatgtgacg tcatcacgct gtttcccgag
     2521 atggtgacgc ccgtgttgga gagcagcatg cttaaacggg ctcaagagaa gggcctgctc
     2581 gaggtcaagg tgcacaactt gcgcgactac accttggata agcacaaagt cgccgacgat
     2641 ctgccgtacg gcggcggcgc cggtatggtc atgaaggcgg agccgattct gcgcgcggtg
     2701 gaacagatcc gccagccata tgagcagcgc ggcgagaaag tccgggtgtt gttgccctct
     2761 ccgcaaggcc gtccgttcac ccaagcctac gcgcaagggt tggcggctga ttcacgtccg
     2821 atgctcattg tctgtggaca ttacgagggc atcgacgagc gtgtgcggat tgctctcaaa
     2881 ccggaggaag tctctgtcgg agactacgtg ttgacgggcg gcgagttacc agcgcttgtg
     2941 atcattgatg cggcagcccg actgatacct ggcgtcctcg gcgctcctga atccgtcgtc
     3001 gaggagtcgt tcagcgaacc tcttctggag tatccgcact acaccagacc tggcgaggtg
     3061 cggggaataa cggttcccga gatattgctc tcgggacatc acgaggcgat tcgtctctgg
     3121 cgccgcaagg aagcgctacg gacgacctat gagcggcgcc ccgatctgtt aaaggatcgg
     3181 cggttgaccg cggaagacca acagttattg gacgacatca tacgcgaagg cgtgcgcggc
     3241 acgcccgtgc catgtgggga ggaggggtga agtcatgaat cagctagaac ggatacagcg
     3301 gtcattgcag aaaaaagtgg tcccgcgttt tgagatcggg gatacggtgc gggtacacgt
     3361 caaagtggtt gaaggagaaa aagagcgcat tcaggtctat gaaggggctg tgattgcccg
     3421 taaggggagc ctcaatacgg aaatgttcac ggtgcgcaag gtttcctacg gtgtcggcgt
     3481 cgagcgtatt ttccccgtcc attcaccaat cgtcacgaag atcgatgtcg tccgtcaggg
     3541 aaaagtccgg cgcgccaagc tgtattatct acgcggcaag aagggcaagt tctcccgggt
     3601 tgaggatcgc gaattcgtgg gagagagcaa ggcgagcgcc ggctctccgc gcgtaactga
     3661 gccagcggaa gaaccggttg aagcagccgc tgcgaaatcc tgaccgacat catccattct
     3721 ccggccacga gggtcgcggg tgaccgcacg catactcaac ttcgtgggtc ccacaaatga
     3781 attcgaattg gaagcgcggc ggtgcggcta ccgccgtgtc gccggcctgg atgaagccgg
     3841 ccggggtcca ttggccggtc ctgttgtagc atccgccgtc gtcctgccca gccggtgccg
     3901 tctgatcggc tgcgatgatt ccaagctgct ttccgaatcc gaacgcgaac aactgtacgt
     3961 ggtgattagg aagcgggcgg tcagcatcgg cattggttgt gcgaccgagc aagagatcga
     4021 ccggttgaat attctggaag ctacgcgact ggccatgcat cgtgccctcg cagggctcat
     4081 gccgaagcct gattgcctgc tgattgatgc gatttcgctt ccagcctgtc ccatacccgt
     4141 tcgttcgatc atcaaaggtg acgcgctatg tatgtcggtg gccgcggcgt cgatcgtagc
     4201 gaaggtcacc cgagatcgtc tcatggccga atatcatcga gtttatccgc attacaattt
     4261 tctctctcac aagggctacg gaacggaaga gcatttgctg cagcttgcga cacatgggcc
     4321 gtgccagatc catcgccgga cctatgcccc cgtggctgac gtgattgccc ggtcccgtga
     4381 tgttctgcct cagcacgtcc agcaggagct cgacggacaa tagcgtgaca atccttgacc
     4441 ctagacgact ctttgggcaa gaaggagagg agacggccga acgctatttg ctccagaagg
     4501 gctaccggat tgtcgccaag aacctgcgca cgtcccttgg cgagttggat ttggtggcgg
     4561 aggacggcga cgtgctcgtg ttcgtggaag tcaaggcacg ccgaacgacg gattttggcg
     4621 gggcggtcca tgccgtacac cgacagaagc aaactcaatt ggtgcgcttg gcggcgcagt
     4681 tcctcgctcg gcaccattgg tccaaccgcc cttgtcggtt tgacgtcgtg ctgcttcaga
     4741 gctcgcagac agcaggggtg caggtcgacc atattcagaa tgcatttgag gtcccagggg
     4801 acgatttgcg ctggtgaaaa tgcgatgatc caactcgtcc atgtcacgaa gcagtacgat
     4861 cggcggcccg ccctttgcga cgtcacgctt gaaattccga agggcgaatt cgttctatta
     4921 atgggtccga gcggagccgg gaaatctacg ctgctccgta tgctgatcgg cgctgagcgg
     4981 ccggatgccg gccaagtgtt cgtgaacggg aagaacgtca cgacgctcaa gggctccgaa
     5041 attccctacc atcggcgcaa ggtcggaacg gtcttccagg attttcggtt gctggagaaa
     5101 aaatcggtgt tcgacaacgt ggcactgcca ttgcttgtcc aaggaatctc ccgcaccgat
     5161 atcaagcgca aggtgacgga agcgttgcgg gcagtcggcg tggaacacaa gcgtgaccag
     5221 ctgcccatga gtctgtcgac gggggagcag cagcgcgttt gcattgcgcg ggcgatcgtc
     5281 aacggaccag tcgtgctgtt ggcggacgag ccgacgggca acctcgatcc cgcactaact
     5341 ggcgaaatca tcgacttgtt caaactcatc aatgcccgtg gaaccacggt ggtagtcgcc
     5401 acgcatgatc cgtccgtgat gacccaagtc aatcgccgag ttgtcacgct cgtgcgaggg
     5461 tctttgatgc cggagcagcg ggtcggtgta tgaggacgct gtcttatcta ctgcgggagg
     5521 cgtgggccaa catttggacc aatcgcacga cgaccgtcgt ggcgattttc accacggcat
     5581 tcacgctggc atgtgtcggc gtctttctac tgctgtacgt caatctgcgg gcggccgccg
     5641 ggtggctgca ggacgatatc aaggtcatgg tgtacttgtc cgaagggatt gctggctcca
     5701 cggtgtcgga catcgaacag caattgcgcg ccgatcgggc ggtaggcgcg ttgcatttca
     5761 tttccaagga gcaagcgctg ggagaattca gagctcagtt tccgtcggat tctcatctcc
     5821 tggaagggtt ggggcaaaat cctttgccgg cctctttcgt cgtcacgctg gcaccggctt
     5881 ttcgctctcc ggatgcggtc agaaggtggg cggaacgcgt cgccgctgtc gccggcgtcg
     5941 cgaaagtgga ttacaatcag gactggcttg atgccctctc gacagtcatt cgctcgatcg
     6001 aagtagtcgc catcgggatc gggctcatcc tctcggctgc ggccgtgacg atcatcgcca
     6061 acaccatcag actcaccttg ttcgcacgcc gggatgaaat cgctatccta cggctcatcg
     6121 gcgcgacgaa gaccttcatt cgcattccct atttgctgga gggtgcagtc ctgggcgcgt
     6181 tgggcagcac ctgctctctg ctcatgctca aagcgttgta tgagctgttc agacaacaga
     6241 tgcgaacgac cgggcgtctg cgggggctgg aacaactcac cgtgttcttt cctctctcga
     6301 tctgtcttgt gcttgtcgcc attgggatca tcctgggctg cgccggaagc tttctgtccc
     6361 tccgacggtt cgacgaggcc aaagtatgag gcgggtaggg cctccagcga cagccctctg
     6421 tctgttgatc gccgtatgcc tcgtgccgcc ggcggtctgg gccgattcga ttaatgagaa
     6481 aatcgacaaa gaacgaaaga ctttggagca gctgaaagac cagattgaag aaaaaagacg
     6541 ccaggccgac gaagcgagca agaagcgcga gtccattctt cagggcattc agacgctgga
     6601 cgagcgacac atgcgctatc ggcagtcccg tcaggacatc agccgaaagc tgcgaaaaaa
     6661 agatcaagag atcgagacga tcaacgccca gattgcgcgc ttgcgcgaag gtgtgcaggt
     6721 caggcaacag gcgatcttgg ccaggctccg tgtgcagtat atggaggggc ggttcggaca
     6781 ttggaaaccg ttgctcgcgt ccgattcgta cggggatttc caacggcgcc tgcgctatct
     6841 gtcggcggtt tcggagcggg actacagtct gatcgagacc tttaagagtg acatggccac
     6901 catgcaggaa gccgaacggc agcgggaggc ggcgcgcgtc ggcatgctgg cctacaagcg
     6961 cagcacagaa aagcatctgg aggagatcaa cgccgtcaag aaggaaaaaa agctgtacct
     7021 gaccaagctg acgcaggaaa aggagtccta cgagcgggcc ttgcaggagc tcgagcgatc
     7081 cgcctcacgg atcgacagcc ttctccgcga gctggaacag cggcgtcgag ccgcggccgc
     7141 caagcctccc gcaggacacc tgcagcgcgg cgtgaaagga ggcttgccgt ggcccgccga
     7201 aggcagcgtg ctgacctact tcggccgcca gaagcatccc acattcaaca cctacgtgca
     7261 gcgcaagggc atcgagatca agacattgga agggagcgcc atccatgccg tgatgcccgg
     7321 gacggtcgtc tatgcggact ggttgaaagg ctacggactg gttataatcc tcgatcatgc
     7381 caacggcttc ttttccctct atgcccacgc gtcgaaaatt ttgacctccg taggggcaca
     7441 ggtggcgtca gggcaggcaa tcggggagac cggtgacacg ggaatgaccg gcgaaaatac
     7501 cttatacttc gaattgcggg aaggggccga agcaatcgat cctcttcaat ggcttgcgag
     7561 acggtaagcg aaaggtgatc aggcaggcga aaggacgcaa atgatggaac acaaccaacg
     7621 acggcgactt tggatgttgg gcccgatggt cgtggtggcc ctgctgtccg gcgtgttaat
     7681 cggtaaggga tgggaacgga ccgggcatgc ttcggaaacg tatgaggagc tgagaacgtt
     7741 ctcggaggtg ctgacgcagg tccaaaaaaa ctatgtcgat gacaccaagg tgaaggacct
     7801 tgtgcaagga gctattcggg gcatgctgtc gacgctcgat ccccattcgg cttacatgac
     7861 gccggaaatg tacaaagaga tgcaagtcga aaccaagggc gagttcggag gggtcggcat
     7921 tcagatcggc gtcaaagaga accggttggc cgtgattgcc ccgatcgatg gcaccccggc
     7981 acagcgggcc ggcatcaagg ccggtgacta catcaccaag gtcaatgagg agtccaccaa
     8041 ggatctttcg ctcatggatg ccgtgcaaaa aatgcgtgga cccaaaggaa cgaaagtaaa
     8101 tctgaccatc caacgagacg gcacctcgga tcccctacag ttcactcttg tgcgggatac
     8161 catcaagatt gaaagcgtga aatcgaaggt catggacaac atcggatacg tgaaactcac
     8221 gcagtttcaa gaggcaaccg gccgtgattt gggtcgggtg ctgaagcagt tcaaggacca
     8281 gaaggttcag tccaccatcc tcgacttacg aaacaatccc ggcgggcttt tgacggcggc
     8341 ggttgaggtg tcggagcaat tcctgccgaa cggaaagctg gtggtctata cgaaaggccg
     8401 agagagcaag aaagacgaat ggttcgccaa gggcagagat caaatggatg attcaccgat
     8461 gattatcctc gtcaatgaag gatcggccag cgcgtccgaa attgtggccg gcgcgttaca
     8521 agactatgga cgggctgtca tcgtcgggac aacgtctttc ggcaagggat cggtgcagac
     8581 gattttgccg ctcggggacg gatcaggatt gcggctcaca acagcgaagt attacacgcc
     8641 gaaagggcgg tcgatccaat cgaccggcat cacgcccgac attgtcatca agccgcagcc
     8701 tgcaaccacg gtggcgaagg ccgaaacaaa accgggtgac aaagaaccgg acaacaagac
     8761 atcaaaatcg ccgatcacgg gcgggccggg gaaagagcag tctgccgccc ctccaaaaga
     8821 cgacgccgtg caacgaaacg gggcaacgcc cggggctgct cctcttgatc tgtccggcga
     8881 gccgtcgctg gatcaggacg tgcagttgca gaaagccgtg gaactgctca aaacctggaa
     8941 gatatttaaa gagctccggc cgtcctgatc agggcctccg gcgtcggagg tcttcgatgg
     9001 ccgtgagcag gtcctcatcg gaaccggaaa atcccggcac cgttctgagc atgcgcgatc
     9061 gtacgagctg ttccaagtcg gccagagtgc caatattcag ccggctgtgc aggtaggcga
     9121 ccagcgattc ggagagaccc ggtaagcctg cccactctct gatatgctcg ggcaagggga
     9181 ttctgagagt ttccaggaga gcgatcttcc ctgtcctgag aaattcttga attttctccg
     9241 ataagtccct gccgatgccc tcgatctctt ccaacgcttg gcgttgcgcc acttccgcaa
     9301 tgtcttcatc gagcgccaac aaggcgtcgg ctccgcgccg ataggcccgc actcgatagg
     9361 gattcgcacg ctgcgcggcc aggagctcgg cgatggatcg aaagagaccg gcaagctgca
     9421 cgttgtgctg tgggatgggc atcgggtgtt ccggcgttct attctgcagc gttcatcgcg
     9481 agagtggcaa ctaggaagcg ggtgcgcgtt gattgacaac gggaattagg gttccctagg
     9541 attgtagtca atggctgagc cattgcagat tcaggtcaac ggcgaatccc gcgattgccc
     9601 ctccggagcc acggtcggtg atctgttgcg ggaactggac gtcaagactg agcgggtggc
     9661 tgtggaatta aacctggaaa ttttggatcg ccgggaattc gatcggcgtg gcctcaaagc
     9721 aggggatcga gtggagattt tgagcttcat cggcggcggt ggtccggacg acggctggat
     9781 aaggagggtt ccgacccatg cacaatgacc gactggtgat cgccggccgt gagttcaaat
     9841 ccaggctgtg ggtgggaacc ggtaagtaca aagattttgc cgagaccaag aaggcgatcg
     9901 aagtctccgg agcggacgtc gtgacggtgg cggtgcgccg cgtgaacatc acggatcgct
     9961 cgaaagagaa tctgctcgac ttcatcgatc cgaagaaata tacgatcctt cccaatacgg
    10021 ccggttgtta cacggtggag gatgccgtgc ggtatgcgcg gctcgcacgg gcggccggag
    10081 tgtctgatct tgtcaagctg gaagtgctgg gggatgagaa aacattattt cccgatacgg
    10141 gaggcttgat cgaagccgcc aagattctca tcaaggaagg attcattgtc ctcccttaca
    10201 cgaacgacga tcccatcgtc gccaagaagc tcgtggacat cggctgtcct gccgtcatgc
    10261 cgctggcggc acccatcgga tcgggcctgg ggatacgcaa tccgtataat ttgaaaatca
    10321 tcatggaaac ggtcaaggtc cccatcgttg ttgatgcggg agtcggcacg gcttcagatg
    10381 cggcgctggc gatggagtac ggagccgatg ccgtactcat gaatacggcc at
//
LOCUS       DASSCQ010000032         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_211822, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000032 DASSCQ010000000
VERSION     DASSCQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211822"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFH05_02230"
     CDS             complement(<1..1724)
                     /locus_tag="VFH05_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET6677061.1"
                     /translation="MSVLKLGLWAFIGVLGACALAIVSGLVSAHEKVNGLWLVIAAAC
                     IYVLAYRFYGRWLACRVASLDNHRLTPAVRLNDGVNFHPTNRVVLFGHHFAAIAGAGP
                     LLGPVLAAQFGFLPGFLWLVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHDELGTLT
                     GSATAVAVLFIVVVALAGLGFAVVNALYRNAWGTFTIATTIPIGLFMGFYLQKVRPGA
                     VAEVSIVGVTLLIMTVLFGRVVSQSSYAPWFEYEKATLVWLLAGYGFLASILPAWMLL
                     VPRGYLSTFMKLGVVVLLGLGVVLMAPTIEMPRITVFAAGGGPIIPGSLFPFLFITIA
                     CGAVSGFHALVSSGTTPKMIEQESQAVVGYAAMLLESFVGVMALIAATVLMPGDYLAI
                     NTHLSTDVLTTMGFPPVRIAELSQLVEANVSGRPGGAVSLAIGMASIFSALPGMAGLM
                     AYWYQFALVFEALFILTTIDTGTRVARYLIQEMAGRLYPPFRRFNWLPGVLSSSGFVV
                     GAWAYLIGTGSISTIWPMFGAANQLLGTLALCIGTTVLIKMRKSSYLWITALPMLFIG
                     GVTLTGSY"
     gene            complement(1898..2332)
                     /locus_tag="VFH05_02235"
     CDS             complement(1898..2332)
                     /locus_tag="VFH05_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6677062.1"
                     /translation="MSTYLPAVFSTVRTDGFDRQIDRMLDEALRTFGTAEGAWVPACN
                     AWEDSNGFYVQMALPGWEPKDVTLEVNNQVLSVKGEKTEESSDSRKLLMREIADGRFV
                     RIFKLPTIVDHDKASATYKNGLLTISFPKREEAKCRRIVIEG"
BASE COUNT          703 a          829 c          750 g          545 t
ORIGIN      
        1 tcgtacgacc ccgtcagcgt cactccaccg ataaacaaca tcggcagcgc cgtgatccac
       61 agataggagg acttccgcat cttgatcaac accgtcgtgc cgatgcaaag tgcaagcgtt
      121 cccaggagtt gattcgcagc accgaacatc ggccagatgg tcgaaatgct gcctgtcccg
      181 atgagatagg cccaggctcc gacgacaaag ccactactgg acagcacgcc tggcagccag
      241 ttaaaccgac gaaacggcgg atacaggcgg ccagccattt cctgaatgag ataccgggcc
      301 acgcgagtcc cggtatcaat ggttgtcagg atgaacaacg cttcgaatac caacgcaaac
      361 tgataccaat aggccatgag tccagccatg ccgggcaatg ctgagaaaat cgaagccatt
      421 ccaatagcta gagataccgc tccgccggga cgccctgaga cattcgcctc aaccaattga
      481 gacagctctg caatccttac cggagggaag cccatcgtgg tgaggacatc ggtggaaaga
      541 tgcgtgttga ttgcgagata gtcgcctggc atcaacaccg tggccgcgat caatgccatg
      601 acgccgacga aactttccaa gagcattgcg gcatacccca ccactgcttg tgattcctgc
      661 tcgatcatct tgggcgtcgt ccccgacgac acgagcgcat ggaagccgga gaccgctccg
      721 caggcgatgg taataaagag aaacgggaag aggctgccag gtatgatcgg ccccccacct
      781 gcggcaaata cggttattcg tggcatctca atcgttggtg ccatcagaac cacgcccagt
      841 cccagcaata ccacgacgcc gagtttcatg aaggtcgaga gatacccacg gggcaccaac
      901 aacatccatg cgggcagaat ggaagcgaga aacccatagc cagccagcag ccacacgagt
      961 gtggccttct cgtattcgaa ccacggcgca taactcgact gtgacacgac gcgaccgaac
     1021 aggacggtca tgatgagtaa cgtcactcct acgattgaaa cttcggcgac cgctcccgga
     1081 cggacctttt gaagatagaa tcccatgaat aggccgatgg gaatcgtcgt cgcaatggta
     1141 aatgtccccc aggcgttgcg atacagtgca ttgacaacgg cgaaccccaa ccccgccaag
     1201 gcgacgacca caatgaacaa caccgctacg gccgtggcac tccctgtgag cgtaccaagt
     1261 tcatcatgag cgatctcggg tagcgagcgg ccattgcgtc tcatggacgc caccaaaatg
     1321 atgaagtcct gtaccgctcc agccagcacc gcgccgatca ccagccagag aaatccgggg
     1381 agaaatccga attgcgccgc gagcactggc ccgagcaagg gaccggcccc ggcgatggcc
     1441 gcaaaatggt ggccgaaaag taccacccta ttcgtgggat gaaaattcac tccatcattc
     1501 aatcgcaccg caggagtgag ccgatgattg tctagggaag cgacgcgaca agcgagccag
     1561 cggccataga aacggtaggc cagcacatag atgcaggccg cggcgatcac caaccagaga
     1621 ccattcacct tttcatgcgc agagaccagt ccgctcacta tggcaagcgc acaggccccg
     1681 agcacaccga tgaaggccca tagtcccagt ttcaacacgg acatgggggc aatcctagca
     1741 caaatagcga atgatcagag gctggcgagg cgcgacgact tgagcgaggc agggaattct
     1801 gttagtcgat tgctggctgg taacttaatt gggtcgagaa cgtcgccccg cacggccgga
     1861 tgcatcggcc ggcgaggcgt tcagtcggtc agcggaatta tccctcgatg acaatccgtc
     1921 ggcacttcgc ttcttcgcgc ttggggaatg aaatcgtcaa gagaccattt ttataggtag
     1981 ccgacgcttt gtcatgatcc acgatcgtcg gcagcttgaa gatccgcaca aagcggccat
     2041 ctgcaatttc acgcatcagg agttttcgcg aatcgctgct ctcctccgtc ttctcaccct
     2101 tgacggaaag cacctggttg ttgacttcca aggtgacgtc tttcggctcc cagccgggaa
     2161 gggccatctg aacgtaaaat ccgtttgagt cttcccaggc attacaggct gggacccagg
     2221 ctccttcggc cgtgccaaac gtacgaagag cttcgtctaa catccgatcg atttgccgat
     2281 caaagccatc cgttcgaacg gtcgaaaaca cagcgggtaa ataggtgctc ataacgctcc
     2341 ctcctcctct ctgtgagagg tctggttatg ggttttagat tggtttgaat cggcactgcc
     2401 gacgaatcac acctgctcag gcctctgttc ggatatatgc cgtagtgccg agtcctctgc
     2461 ccgaatcaac cacaaaggta attttgcgat gcgcaaagtc aagagggtag gcaagacgcc
     2521 acacgaagtt catcgaggct gaatatcctg cttgaggagg gaaagcaccg tcacctgatc
     2581 aaccgatccc ctctgaagag ggaggaaatt ctcggcaaaa gcggggaaaa tttccgtctg
     2641 ctttttcatc ccggcgtctc ttttgcctct tccgtcatgg ctgacagtgc aacctatccg
     2701 ccaagggctt gtatgtgcag cggaatctta tgcccttctt tgtaggtatt cttctggcca
     2761 ctgcattgca cattccctgt caatgctcca tgtccgggcg aacgacctct ctgccaagga
     2821 gaagacc
//
LOCUS       DASSCQ010000033         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1166955, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000033 DASSCQ010000000
VERSION     DASSCQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166955"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /gene="waaF"
                     /locus_tag="VFH05_02240"
     CDS             <1..1636
                     /gene="waaF"
                     /locus_tag="VFH05_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET6677063.1"
                     /translation="CEPALRSLKRLFPQAAVTLLVKPSVAGLFERHPSVDRLMLYEDN
                     RRHAGLSGKWRLACELRRSGFDLAVLFQNAFEAALLAAAAGIPRRYGYATDGRTLLLS
                     EPVAKPDRHKVVHQVQYYWDMLKPLGLTGEPVSPELVVSPQEEEAMAGRLKQVGITTE
                     DVLIGVNPGSTYGGAKRWFPERFADVAERLCRRLNGSGRRAAVVLLGARGEEPLGRAI
                     ASHLTAPTAVLSGSTTIRELMAATKRCTLLVTNDTGPMHIAAAFGVPVVAIFGPTDWR
                     TTSPFGVTHALVRHPVECAPCLLRDCPIDHRCMAGVTVQDVYAAANKLLTVPESIAEQ
                     GRGSHVRFGPSPHGILEGYTVFLDRDGTLNEDPGYLRSAADVKLLPGVGPALARLKSA
                     GARLVVVTNQSGVGRGFLTLKDLEAIHARLEGLLEQHEAALDAIYFCPHHPDDRCRCR
                     KPARGMVDRAASELQLDLRRSYLIGDHVSDMQLAKSVGAKPVLVTTGKVDAPAVEKLR
                     AAQALPDKVVSSMPEAVEWILNDAAARAAADPAKES"
     gene            1667..2776
                     /locus_tag="VFH05_02245"
     CDS             1667..2776
                     /locus_tag="VFH05_02245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6677064.1"
                     /translation="MKIFIAESSTAVGGQELAVILHAEGLLKRGHDLRLLLEPQSPIA
                     NMAMDKQLPVILMTMRKSRYPAAILSLRALLAHHRPAILQVNSSRDSWIGAMAARLVR
                     PRPKLLRIRHISAPLNRNLMTRILYRHLFDMVVVTGGDKTRRELVERDGLAAHRVAAF
                     PIGLDVEYFRPAPPDRDLRLEFGLPKGHLLVGLISYLRSYKGHEYFIEAARIISAQRD
                     DVTFIIAGEGPEEQAIRTRIERLGLSSKIRLLGFREDLLNVFRSCDVFAIPSVEGDTI
                     PQVLMQALAVGIPVVSTTVGSIPDVVIEGKTGFVVPPRDAGALAERIAKLLDDATLRT
                     RMGIEGRSLVQRSYSLEKMLDRMEGVYHQLAEAPV"
     gene            2867..>3277
                     /locus_tag="VFH05_02250"
     CDS             2867..>3277
                     /locus_tag="VFH05_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6677065.1"
                     /translation="MAAIRINTVTAGPLPISIYVIAYNDEPNMRACLESVAGWAAELI
                     VVDSHSTDRTTAIAQEFTDKVYQVEFTGFGALRNQAVAFCSHEWIFSLDTDERMTPEL
                     KQEIEQVVGQPNASDAYFVPRKNYFLGRWIKHCGW"
BASE COUNT          646 a          953 c          996 g          682 t
ORIGIN      
        1 gtgtgagccg gcgctgcgta gcctcaaacg actcttccct caggccgcgg tcacattatt
       61 ggtcaaaccg tcggttgccg gcctgtttga gcgtcatccc tccgtcgatc gtctcatgct
      121 atatgaagac aacagacgtc acgcggggct gtccggaaaa tggcggttgg cctgtgaatt
      181 gcgccgcagt gggttcgatc tggcggtgct gtttcagaac gcgttcgaag cagcgttgct
      241 ggcagcagca gccgggattc cccggcggta tggctatgcg acggatgggc gcaccttgtt
      301 gttatcggag ccagtggcga agcctgatcg acacaaggtg gtccaccaag tgcagtacta
      361 ctgggacatg ttaaaaccac tgggcctaac cggtgagccg gtgtccccag aattggtcgt
      421 ctcgccgcaa gaagaagagg ccatggcagg acgcttgaag caggtcggca ttacgacgga
      481 ggatgtgctc atcggcgtca atcccggatc gacatacggc ggcgcgaagc gttggttccc
      541 ggaacggttc gccgatgtgg ctgaacgctt atgccggcgg ctcaatgggt ccggccgacg
      601 agcggcggtc gtcttgctcg gcgccagggg cgaagagccg ttgggacgtg ccatcgcgtc
      661 acacctgacc gcgcccacgg cggtcctttc cggatccacg acgatccgtg agctcatggc
      721 ggcaaccaag cgatgtacgt tgctcgtaac gaatgacacc ggcccgatgc atatcgccgc
      781 agcgtttggc gtccccgtgg tcgccatttt tggtccgacc gattggcgca cgacgtcgcc
      841 attcggcgtg acgcatgcac ttgttcgtca tccggtcgag tgcgctccgt gcctgctgcg
      901 tgactgtccg atcgatcatc ggtgcatggc cggcgtgacg gtacaggacg tctatgcagc
      961 agcaaacaaa ctcctcactg tcccggagtc catcgccgaa cagggacgtg gttcacacgt
     1021 ccgcttcgga ccttcaccgc acggaattct tgaagggtat acagtgttcc ttgatcggga
     1081 tggcaccttg aacgaagatc ccggctatct cagatcagcg gcagatgtga aactgcttcc
     1141 gggagtcggc ccggctctgg cgcgtctgaa atcggcagga gcccggctgg tagtggtcac
     1201 gaaccaatcc ggagttggac ggggattcct gacgctcaaa gacctcgagg cgattcacgc
     1261 acgcctggaa ggtctccttg aacaacacga agccgcgctc gatgccattt acttttgccc
     1321 gcaccatccc gatgaccgct gccgttgtcg caaaccggcc cgggggatgg tcgatcgtgc
     1381 tgcgtccgaa ttgcaactgg accttcgacg gtcctacctc atcggcgacc acgtcagcga
     1441 catgcaattg gccaagtcgg ttggagcgaa accggtcctg gtcacaaccg gtaaagtgga
     1501 cgcgccggcc gtcgagaaat tacgggctgc gcaggcactc cctgacaagg tcgtgtcgtc
     1561 gatgcctgag gcggtggaat ggatactcaa cgacgccgcg gcccgagcgg ctgccgatcc
     1621 tgctaaagag tcatgatgtt gggtctcagg tcctgagcag ccctacgtga aaatcttcat
     1681 cgcagaatcc agcacggcgg ttggtggaca ggagcttgcg gtcatcctgc atgcagaagg
     1741 gctcttgaag cgcggccatg atctgcgcct gcttctggaa ccgcagagcc cgatcgccaa
     1801 catggcaatg gataagcagc tcccggtcat actcatgacc atgcgaaaat cccgctatcc
     1861 cgcggccatc ctctcgttac gagccttatt ggcgcatcat cgccccgcga ttctccaggt
     1921 caatagctca cgggatagct ggatcggcgc gatggccgca cgattggtcc ggccccggcc
     1981 caagctgctt cgaatccggc acatctctgc gcccttgaat cgcaatctca tgacccggat
     2041 tctctaccgc catctgtttg atatggtggt ggtgaccgga ggggacaaaa cgaggcgcga
     2101 gctcgtggaa cgagacggac tcgctgctca tcgtgtcgca gcgttcccca ttggtctgga
     2161 cgtcgagtat tttcggccgg ctccgcctga ccgggacctg cgtctggagt tcggcctgcc
     2221 gaaagggcac ttgctcgtcg gcctgatttc ctatctccgg tcctataagg gacatgaata
     2281 tttcatcgaa gcggcgagaa tcatctcggc ccaacgcgat gacgtcacct tcatcattgc
     2341 tggggaaggt cccgaagagc aagcgatcag gacacggatc gagcggcttg gtctctcttc
     2401 caaaattcgg ctgctgggat ttcgcgagga tctcttgaac gtatttcgtt cgtgcgacgt
     2461 gtttgccatt ccgtctgtgg agggagacac gattccgcag gtcctcatgc aggcgctggc
     2521 cgtcggaatt ccggtcgtct cgacgacggt cgggtcgatc cctgatgtgg tgatcgaggg
     2581 gaaaacaggc tttgtcgttc cgccccggga cgcaggcgcg ctggcggagc ggatcgccaa
     2641 gttgttggac gacgcgacgc tccggacacg gatgggcatc gaagggcgct cgttggtcca
     2701 gcggtcttat tcgctcgaaa aaatgctcga ccggatggaa ggcgtctatc atcagcttgc
     2761 cgaggctccc gtctgataag gccggagccc tgattggagg acgtacagca atgactcgaa
     2821 ggtctcttgc cgccaagatg agactcaatc aacacaaaag ttggtcgtgg cagccattcg
     2881 gatcaacact gtgacggccg gtccactccc aatatcaatc tatgtcatcg cctacaacga
     2941 cgagccgaac atgcgcgcgt gcctcgagtc ggtggccggt tgggccgctg aactgatcgt
     3001 cgtcgattct cacagcaccg atcggacgac ggcgattgcc caggagttta ccgataaggt
     3061 gtatcaagtc gagtttacgg gcttcggcgc tctccgcaac caggccgtgg ccttttgttc
     3121 gcacgaatgg attttcagtc tcgataccga tgagcggatg actcctgaat taaagcagga
     3181 aatagaacag gtggtcggac aaccgaacgc gtccgatgcc tattttgtcc ccagaaagaa
     3241 ttacttcctg ggacgctgga tcaagcactg cggctgg
//
LOCUS       DASSCQ010000034         7953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1377442, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000034 DASSCQ010000000
VERSION     DASSCQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377442"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            684..890
                     /locus_tag="VFH05_02255"
     CDS             684..890
                     /locus_tag="VFH05_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677066.1"
                     /translation="MSEWHLVSVLVALVIGFILGLIYALRLYKQIEADCDREWAKRHG
                     MREMKPKYSEFPMRINPILRRQRL"
     gene            complement(1075..1164)
                     /locus_tag="VFH05_02260"
     tRNA            complement(1075..1164)
                     /locus_tag="VFH05_02260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1128..1130),aa:Ser,seq:tga)
     gene            complement(1211..2497)
                     /gene="serS"
                     /locus_tag="VFH05_02265"
     CDS             complement(1211..2497)
                     /gene="serS"
                     /locus_tag="VFH05_02265"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET6677067.1"
                     /translation="MYDLRDLRDNLDQIKGRLGTRSRDVQWDTLRKLLEERRGLTVQV
                     EHLRADLKKGSDEVASLKRQKLPSEAAVAAMKQLGERIAEVESALRIIEEQLDEIALR
                     IPNLPHQSVPAGTGPEHNVEVRRWGAPPTFSIPPQTHWDLGERLGILDFDRAAKIAGA
                     RFAVLTGDGARLERALINFMLDLHTTEHGYREVLPPLMVNRSAMTGTGQLPKFEEDLF
                     RLRDEDYFLIPTAEVPVTNLHRDEILSESALPIRYTAFTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFTKAEHSYEELERLTGHAETVLQRLGLHHRVVTLCTGDMGFSAAKTY
                     DLEVWLPSQNQFREISSCSNFEAFQARRAAIRWKGAGGKKDSRAEFVHTLNGSGLAVG
                     RTLVAILENYQQADGSVIMPEALRPYLRGMDQIRQE"
     gene            2604..3626
                     /locus_tag="VFH05_02270"
     CDS             2604..3626
                     /locus_tag="VFH05_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET6677068.1"
                     /translation="MRNMRAMSNPPNPYESTHRELLEPAPSARLELYDDETREILSRN
                     DSPDLSFRWSVNPYRGCFHACAYCYARPTHEYWGFGAGTDFESKIIVKRHAPALLRRA
                     FEKPSWKGELIVFSGNTDCYQPIEASLELTRSCLEVCAEYRNPVAIITKGVLVLRDLD
                     ILKRLQQEAWVRVYCSIPFADDEVARRVEPQAPTTRKRFEAMARLSDAGIPTGVSIAP
                     IIPGLNDEDIPDLLHRARQAGARQAMHTLLRLSTSVESIFLERMTEAFPERIGKIIHR
                     IREVRGGAMSDSAFFSRQHGHGPYWDAIERLFEVAKRKAGFEESDHEHVPMTFRRPGR
                     EQARLF"
     gene            3646..4014
                     /locus_tag="VFH05_02275"
     CDS             3646..4014
                     /locus_tag="VFH05_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6677069.1"
                     /translation="MKHNPGFLKLVEAARARVKTCTVAEAKARLDRGEVTHFIDVRED
                     HEFGNEHAKGARHVGKGIIERDIETLVADKRASIVLYCGGGYRSALAADALQQMGYTN
                     VTSMDGGIKAWREAGYPMES"
     gene            complement(4054..4614)
                     /locus_tag="VFH05_02280"
     CDS             complement(4054..4614)
                     /locus_tag="VFH05_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6677070.1"
                     /translation="MRIAKTLRGQTVLVLAAVGLWVLGLTCSAAAAEFKMGVIDPQAV
                     LEKSKAGKKALDGLKEYVSTRQKLLSRDEEELRNTEKTLKEAGAKLSEGEKKEKEAQF
                     RSKIQDYQKRAQEFNQELQGKQKELVDDYMKRIASATQTVAEKGGFSIVVDKGSEQTI
                     RIVIYSKDAIDLTDQVIKEFDRVNSK"
     gene            complement(4754..6262)
                     /locus_tag="VFH05_02285"
     CDS             complement(4754..6262)
                     /locus_tag="VFH05_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6677071.1"
                     /translation="MADPLRASDDGAPNAPAVQPILTHEKSLTKKVAKGSEAGDIYDQ
                     QILMLGTQLVTQISILIKMSRLHGRTNSALETPVESALTVIKTLAQDEPIPVRLQNDF
                     LFLGQRHLKVTAAQMPVFSTVIDTFNQWCIGGVSFSKDVESRDFREFIFQLLAFDAAT
                     SSIATLRTALEQKAVKGVEIEERRVLRGKATAKTRYVNAASAVGTLVQDSREKGTIGF
                     KQAKRAIQSIVDLMMNDEASLLGLTTLRCHDQYTHNHSVNVSLLSMALGNRAGYPKVE
                     LADLGLAALFHDLGKATIPMEVLNKPGEFTDEEWAMMRNHPTEGALSLAKMRGITNLP
                     GRMASASFEHHMNMDFSGYPKLNLSWKISMTGRILMIADCYDAMTSSRVYRREPMSPS
                     KVLGFMFAKAGTSFDPTLMKLFMTCVGIVPIGSLVLLDSNELAVVLKPAADPSDAEYP
                     FVKVITDAAGEAIDNGREIDLREKNENGDYCNYIVRLIDNSEHKFDTSRYFV"
     gene            complement(6292..>7953)
                     /locus_tag="VFH05_02290"
     CDS             complement(6292..>7953)
                     /locus_tag="VFH05_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6677072.1"
                     /translation="LLKSLDRASKNVRTFGHQNIVAKKFFDQFYTELHTHLEQYSVLA
                     FIIQREGLFFKEGCVYNSQAGESSENFAFKLYSDGIREITFHQGIEQEDVLFFFDALW
                     DTGGASGTEDDDIVTRLWSRNLPTLTIVTADEVMKLSEVDDVLTPQRQVPPQGALREI
                     LAEINAKGRSEAKGDQQRKPRLSTNVMGYEVTEVEMAALGRDIEAESQRDSILYLLDI
                     LTAVLSSERSPDLLNKLFDIYEGILKSLFQGGHWTIVEHVLGLLTEAEEVRPDLNEAH
                     KQKLHNMFDQLGSPELVTLIKQYLNTADKPRTEGLETVLLMMKPSAMPALCSLLGDLE
                     HAAHQALIANALVELAKDSPEIVVKHLTDRRVSFVRNLLGIITRWNNPKLAESVEKIL
                     RHPDPLIRREAIRTLAGLKPSGSAAKLVPLLNDQDEGVRLATLKPLLTGHYTATFNMW
                     EPIVNAEHFTERDAAEKRNIFHAMRVTTGDDAVPYWGSLLTDWGWTNRKKREDLALLA
                     VDALGKLGTAAAKAALDTGSKKGTAAVKQACATALTGTNKQLRTA"
BASE COUNT         1657 a         2154 c         2284 g         1858 t
ORIGIN      
        1 ccgtgtttcc tgcgtgacag agcacgaaat ctcagagata catctggcac atcagttgcc
       61 ttaggcaagg ttgaaatctg gccgaacaag cgctgctcgt acttgcatac gccagtgacg
      121 tttcaagcct cggtcttaaa accacaatgc ttggggagaa cccatgatct ttgcgtatag
      181 gtttctctgt tctgtggcgt tcggatgttg tctcatctca ttagctgcat gtggagaagg
      241 gggagaagga agcagtgccg gcgccatagc atctgtagcg tgggaccccg tgaatgatcc
      301 tacagtgatt tcatacaccg tgcattatgg aaagcattca agcgggagtg tcggatcatg
      361 caattacgaa aattcagtgg atgtggccga gccttctact gcaatcggag ggcttgagtt
      421 taatgcacag tactattttg cggtcagtgc ctttaatggt gctcacagca gttgttctgg
      481 tgaaataaca gagatgacac ctgctggaca atcgaacggg aatccgatgt agttgacgga
      541 gaggtggagc acacaatgtc ttcgactcct caggagccgc ttcgacctgc cggcaactcc
      601 gcccttgcga cagtcagact gttcagaaaa actcaagtgt accgcacgag acctcatggc
      661 cggcggatgg aggaggtgcc tagatgagcg aatggcacct tgtgtctgtg ctggtcgcgt
      721 tagtgattgg gttcatattg ggtctgatct atgcgctccg gctctacaaa cagatagaag
      781 cagattgtga ccgagagtgg gcgaagcggc acggaatgcg agagatgaag ccgaaataca
      841 gtgagtttcc gatgcggatc aatccaatcc tgcggcgtca gcgattgtga ttaggaaccg
      901 tgaagggaca agcgaagacg cttcctgaca cgaggaaccc atacgttcaa cctcgttctg
      961 cccgagatct ggggagctgc tacacttttg ggccaatcta gagaagactc gggagctcct
     1021 aaggctacag aatttgatgc catgtgggcc gtaccccata aagccgttgt attatggcgg
     1081 agggggcgag atttgaactc gcggacccct tgcgaggtct ccggttttca agaccggcac
     1141 gttcggccgc tccgtcaccc ctccatgttc taattctaat tcagtagtac tccggcgagc
     1201 acgctctatt ctactcttgt ctgatctggt ccattcctcg taaatagggc cgcaaggcct
     1261 ctggcatgat gaccgatccg tcggcttgct ggtagttttc caggatggcc acgagggtcc
     1321 gtccgaccgc gagtcctgaa ccattcagcg tatggacaaa ttctgcccgc gaatcctttt
     1381 tcccgccggc gcctttccaa cggatcgcag cccgtctcgc ctgaaacgcc tcgaagttgc
     1441 tgcaggatga aatttcacgg aactggttct gtgaaggaag ccacacctcc aggtcgtagg
     1501 ttttggcggc agaaaacccc atgtctccgg tacatagcgt tacgacgcga tgatgaagtc
     1561 ccagtcgctg gaggaccgtc tcggcatgtc ctgtgagccg ctccaactct tcgtaagaat
     1621 gttcggcttt cgtgaaggca accagttcca ccttgttaaa ctgatgcaga cggatcaagc
     1681 ctcgggtgtc cttgccgtac gaccccgcct cacgccgaaa gcagggggta aaggcggtat
     1741 atcgaatcgg aagggccgat tcactgagga tctcgtcccg atgcaggttg gtgacgggaa
     1801 cttcggcggt cggtatcagg aaatagtcct cgtcgcgaag gcgaaataga tcctcttcga
     1861 attttggcag ctggcccgtc cctgtcatag ccgatcgatt caccatgagc ggcggcagga
     1921 cttctcgata gccatgttcg gtcgtgtgaa gatcgagcat gaaattaatc agcgcccgct
     1981 ccagtcttgc tccgtcgcct gtcaggacgg caaatcgggc accggcgatt ttcgccgcgc
     2041 gatcgaaatc gagaatgccg agacgctcac caaggtccca atgcgtttgg ggaggaatcg
     2101 agaaggttgg cggcgccccc caacggcgca cctcgacgtt gtgttccgga ccggttcctg
     2161 ctggaacgga ctgatggggc agattgggaa tccgcagcgc gatctcgtcg agctgttctt
     2221 cgataatacg aagcgccgat tcgacttcgg cgatgcgctc accaagctgc ttcatggcgg
     2281 ccacggcagc ctcgctgggc agtttctgcc gtttcaggct ggcaacctca tcggaaccct
     2341 ttttcagatc tgctctgagg tgttccactt ggacggtcaa accacgccgc tcctcaagca
     2401 atttacgaag agtgtcccat tggacgtctc ggctccgggt tccgagtcga cctttgatct
     2461 ggtccaagtt atcgcggagg tcgcgcaaat cgtacatgag aggcgtgttc caatgggcgg
     2521 ctgaaatcta acatcgaggt ggcgaggagt caacgaacgc cgcgtggcga gccccgttga
     2581 cagtggcgcg gcgaagagag accatgagaa acatgcgcgc gatgtccaat ccccctaatc
     2641 cgtatgagtc cactcatcga gagctactcg agccggctcc atctgcgcgg ctcgagcttt
     2701 acgatgacga gacgagagaa attctaagcc gcaatgacag tcccgaccta tcttttcgct
     2761 ggagtgtcaa tccatatcga gggtgttttc atgcctgcgc ctattgttat gcgaggccga
     2821 cgcatgaata ttggggcttc ggggccggaa ctgatttcga atcgaagatc atcgtcaagc
     2881 gtcatgcgcc ggcattgttg cgccgggcat ttgaaaaacc gtcctggaaa ggggagttga
     2941 tcgttttttc cggcaatacc gactgctacc agccgatcga agcctcgctc gaattgaccc
     3001 gctcgtgtct ggaggtctgc gcagagtatc gaaaccccgt ggccattatc acgaagggtg
     3061 tgttggttct ccgcgacttg gatatcctca aacggttgca gcaggaagcc tgggtgaggg
     3121 tgtattgcag cattcccttt gccgacgatg aagtggctcg gcgagttgag ccgcaggctc
     3181 caacaacccg gaagcgtttt gaggccatgg cgcgtttgtc cgatgccgga atccccaccg
     3241 gcgtttccat tgctccgatc atcccaggtc tcaacgacga agacatccca gatctcttgc
     3301 accgcgccag gcaagccggt gcccgacaag cgatgcacac cctgttacgg ctgtcgacat
     3361 ctgtggaatc catttttctc gagcgcatga cagaagcctt tcctgagcgg atcggtaaaa
     3421 ttatccatcg catccgggaa gttcgtggag gcgccatgtc tgatagtgcg ttcttttcac
     3481 gtcagcacgg acacggacca tattgggatg cgattgaacg cttgtttgaa gtggcgaagc
     3541 ggaaggccgg cttcgaggaa tccgaccacg agcatgtgcc catgacgttt cgccgaccgg
     3601 gacgggagca agcacggttg ttctgacggc gaagggagtg atcgcatgaa gcataacccc
     3661 ggatttttga agttggtgga ggctgcgcgt gcacgggtga agacatgcac ggtagccgag
     3721 gccaaggcgc gcctcgatcg cggggaagtc acgcacttca ttgatgtccg cgaagatcat
     3781 gagtttggaa acgagcacgc caaaggggcg cggcacgtgg gaaaaggcat catcgagcgg
     3841 gacatcgaga cactggtcgc agacaagcga gcttcgattg tcctgtattg tggtggaggg
     3901 tatcgatccg cgctcgctgc ggatgccttg cagcaaatgg gctacacgaa cgttacatcg
     3961 atggatggag ggatcaaggc atggagggaa gcgggatatc cgatggaatc ctgacggaac
     4021 ttctccgcga gaagtccgtc aggctaggcg tttttatttg ctgttcaccc ggtcaaattc
     4081 cttgatgacc tggtcggtca ggtcgatggc atccttgcta tagatcacaa tcctaatggt
     4141 ctgttcgctg cccttgtcga cgacgatcga aaaaccaccc ttctcggcga ctgtctgcgt
     4201 cgccgatgcg attcgcttca tgtaatcgtc tacgagctct ttctgtttac cctgcagttc
     4261 ctgattaaac tcctgtgctc gtttctgata gtcttgaatc ttgctgcgaa actgcgcttc
     4321 cttctctttt ttctccccct cgctgagctt ggcgccggct tctttgagcg tcttttcggt
     4381 gttgcgcagc tcttcctcat ccctcgacag cagtttctgt cgcgtcgaga catactcttt
     4441 gagaccatcg agcgccttct ttcccgcttt tgatttttcc agtacggcct ggggatcaat
     4501 gactcccatc ttgaattccg ccgctgcggc cgagcacgtc agaccgagta cccacaatcc
     4561 gacggccgca aggacaagaa ccgtctgacc ccgaagggtc ttcgcgatac gcataatgtc
     4621 ggctccttaa tggatgagta cctaaactcg ttgagaatag ggaagaatag cggcggtgga
     4681 tagcagtgtc aaggagccct ggagcccctg aggccgctcg cagctggagg cacatgactg
     4741 ggaaggcggg tcgtcagaca aaatagcggc tcgtatcaaa tttgtgctcg gagttgtcga
     4801 tcaggcggac gatgtagtta caatagtcgc cgttctcgtt tttttcacgg agatcgattt
     4861 cccggccgtt gtcgatggcc tcacctgccg catccgtaat gactttcaca aacggatatt
     4921 ccgcgtcgga cggatcagca gccggcttca ggacgacggc cagttcgttg ctatccagga
     4981 ggaccaggct gccgatgggg acaatgccga cgcaggtcat gaatagtttc atcaacgtcg
     5041 ggtcgaagga tgtgccggct ttggcgaaca tgaaaccgag gaccttggag ggagacatcg
     5101 gctcccgccg gtacacgcga gacgaggtca ttgcgtcata acagtctgcg atcatgagga
     5161 tgcggccggt catggaaatc ttccaagaaa ggttcagttt gggatagcca gaaaaatcca
     5221 tgttcatatg gtgttcgaat gaagccgatg ccatccggcc gggaagattt gtgatcccac
     5281 gcattttagc caggctcagc gcgccctcgg tggggtgatt acgcatcatc gcccattctt
     5341 catcggtaaa ctcgccgggc ttattcaaca cttccatggg aatcgtcgcc ttgccgaggt
     5401 cgtgaaagag ggcggccagg cccaaatcgg cgagttcgac cttaggatag ccggcgcggt
     5461 tgccgagagc cattgagagt aatgagacgt tcactgaatg attatgcgtg tattgatcgt
     5521 ggcaacgaag cgtcgtgagg cccagtaagg aggcttcgtc gttcatcatg agatcgacga
     5581 tgctttgaat ggcacgtttg gcttgcttga acccgatcgt tcccttctca cgcgaatcct
     5641 gtaccagcgt gccaactgcc gaggcggcgt tgacatagcg ggttttagcc gtggccttcc
     5701 cgcgcaacac gcgccgctcc tcgatctcga cgcccttgac cgccttctgc tcgagagcgg
     5761 tccggagcgt ggcgattgaa ctcgtagctg catcgaaggc gagaagctga aaaatgaact
     5821 cgcgaaaatc cctggactcg acgtccttgg agaatgacac gcctccgata caccattggt
     5881 tgaaggtatc gatgactgtc gaaaacacgg gcatttgggc ggccgtaact ttcaggtgcc
     5941 gctgtccaag aaagaggaaa tcgttctgca gccgcaccgg gatcggttcg tcctgtgcga
     6001 gcgttttgat gacggtcagg gcgctctcca ccggggtctc cagcgctgag ttcgtgcggc
     6061 cgtgcaggcg cgacatcttg atcaggatgc tgatctgggt caccagctgc gtccccagca
     6121 tcaaaatctg ttgatcatag atgtcgccgg cctccgatcc tttggcgacc ttcttggtca
     6181 gcgacttctc atgagtgagg atcggttgga ccgccggggc attcggcgct ccatcgtcag
     6241 atgcgcgcaa aggatcagcc attggaggac atcctcatgt ctcttattgc ttcacgccgt
     6301 tcgtaactgt ttgttggtgc cggtgagagc ggtggcacag gcttgcttga ctgctgcggt
     6361 cccttttttt gagcccgtat ccagggcggc ctttgcggcg gccgttccca gtttgcccag
     6421 cgcatcgacg gccagtaaag cgaggtcttc acgctttttc cggtttgtcc atccccagtc
     6481 ggtcaacaag ctgccccagt atggaacggc gtcgtcaccg gtggtgaccc gcatggcgtg
     6541 aaatatattt cgcttctcgg cggcgtcccg ttcggtgaag tgttccgcgt tgacaatcgg
     6601 ttcccacatg ttgaaggtgg ccgtgtagtg accggtcagc aacggcttga gtgtggcaag
     6661 gcgcacgccc tcatcctggt cgttcaataa gggcacgagt ttggctgcgc tgccgctggg
     6721 tttcaggccg gccaacgttc tgattgcctc ccgcctgatc agcggatcgg gatggcgcag
     6781 gatcttttct actgattccg ccagcttggg attattccac cgtgtgatga tgccgaggag
     6841 attgcgaaca aagctgacgc ggcgatccgt caaatgtttc acgacaatct ctgggctgtc
     6901 tttcgcgagt tcgaccaggg cgttggcaat cagggcttga tgagccgcat gttccagatc
     6961 gccaaggaga ctgcacagcg ccggcatagc cgatggtttc atcatcagca gcacagtctc
     7021 gagtccttcc gtccgaggct tgtcagcggt gttcagatac tgcttgatga gcgtgaccaa
     7081 ttccgggctg cccaattgat cgaacatgtt gtgcagtttt tgcttatgcg cctcgttgag
     7141 gtcgggccgc acttcctccg cttcggtcaa caagccaagc acatgctcga caatcgtcca
     7201 gtgtccgccc tgaaaaagcg atttcagtat gccttcataa atatcaaaaa gtttattcag
     7261 caggtccggc gaccgctcgg atgagagcac agccgtcagg atatcgagca ggtacaggat
     7321 gctgtcgcgt tgggactctg cttcgatatc acggcccagc gcggccattt ccacttcggt
     7381 gacctcatag cccatcacgt ttgtcgagag cctcggtttc cgctgctgat cgcctttggc
     7441 ctcgctcctg cctttggcgt tgatttcggc caagatttcc cgcagcgctc cttgcggcgg
     7501 cacttgtctc tggggagtca gtacgtcgtc gacctccgag agcttcatca cctcgtcggc
     7561 cgtgacaatc gtgagcgtcg gaagattccg tgaccacagg cgcgtgacga tatcatcgtc
     7621 ctccgtgccc gaggcaccgc ctgtgtccca cagcgcgtca aagaagaaga gcacgtcttc
     7681 ctgttcaatc ccttgatgaa aggtaatctc gcggatgccg tcgctgtaga gcttaaatgc
     7741 gaagttctca ctcgactcgc cggcctggga gttatacacg cacccttcct tgaaaaagag
     7801 gccttctcgc tggatgatga atgccagaac gctgtactgc tcaaggtggg tgtgcaactc
     7861 cgtgtagaac tggtcgaaaa actttttggc cacaatgttc tgatggccga acgtgcggac
     7921 attttttgaa gcccgatcga gcgatttcag cag
//
LOCUS       DASSCQ010000035        11697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1224404, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000035 DASSCQ010000000
VERSION     DASSCQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224404"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..202
                     /gene="acpP"
                     /locus_tag="VFH05_02295"
     CDS             2..202
                     /gene="acpP"
                     /locus_tag="VFH05_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6677073.1"
                     /translation="MAEQLGVEEDEVTSEASFVEDLGADSLDTVELVMALEEEFGIEI
                     PDEDAEKILTVGKALDYIKEKS"
     gene            212..1465
                     /gene="fabF"
                     /locus_tag="VFH05_02300"
     CDS             212..1465
                     /gene="fabF"
                     /locus_tag="VFH05_02300"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET6677074.1"
                     /translation="MKSDGAKRRVVVTGLGVITPLGTGVDKTWKAICSGQSGIGRITK
                     FDPAGYDAQIAGEVKDFDPAQFIEKKDIKKMDAFIHYAVAAAQLAADDAGLKVLPEEA
                     TKVGVYIGSGIGGLGSIEHYHTVLKDKGPGRVSPFFIPMTIINLASGQVAIRLGAKGP
                     NSCAVTACATGNHCIGDAFRLIQRGEADVMVAGGAEAAITTLGVAGFAASKALSFRND
                     EPTKASRPFDKDRDGFVLGEGAGVIVLEELDHARLRGVRIYAEVVGYGMNSDAYHITA
                     PSEEGEGAVRCMELALADANMGKDQVGYINAHGTSTMADVIETKAIKQVFGERALRIP
                     VSSTKSMTGHLLGAAGGIEAVFSILAIHHGILPPTINLDHPDPECDLDYVPHKARPAT
                     VNVALSNSFGFGGVNACLLFKKPEA"
     gene            1499..2200
                     /gene="rnc"
                     /locus_tag="VFH05_02305"
     CDS             1499..2200
                     /gene="rnc"
                     /locus_tag="VFH05_02305"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET6677075.1"
                     /translation="MTTAPPLDSLQAALAYRFTNPVLLQEALTHKSFINERKTTGGSH
                     NERLEFVGDAVLSLVVSEQLAALLPQSSEGALSKHKARLVSESMLAHVARHLKVGDCL
                     RLGRGEELSRGREKDSLLADALEALIAAVYLDGGLESSRRVVIGLLERELRQVVTQQH
                     RPGEDDYKTQVQEWCQRRFDSLPSYTVVRESGPDHDKIFEVEVAVNGKVVGRGTGRSK
                     KEAEQSAAKQALHEA"
     gene            2257..2838
                     /locus_tag="VFH05_02310"
     CDS             2257..2838
                     /locus_tag="VFH05_02310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6677076.1"
                     /translation="MQRLVMRLAMAVSVMTTAWTLVGPAGVAGETGAGKAPKAVIKTK
                     FGDMEIAFFPDKAPNHVQNFIKLAKSGYYNGTIFHRVIPGFMIQGGDPNTKDPKKPET
                     YGMGGPSDKLKAEFNDTPHRRGIVSMARSNDPNSAGSQFFIVIKDSNFLDGQYTAFGE
                     VVKGMEVADKIVNLPRNARDLPNERAEMTVVVE"
     gene            2889..3479
                     /locus_tag="VFH05_02315"
     CDS             2889..3479
                     /locus_tag="VFH05_02315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6677077.1"
                     /translation="MLITAMIMAMNVSAGCSGKSDLKAAVPPPDPRPRAIIKTRFGEM
                     ELKLYPDLAPNHVENFIKLAKSGFYNGTIFHRVIPGFMIQGGDPNTKYSLRKDTFGQG
                     GPKDEKGDPILLKAEFSDTPHKRGIVSMARAGEPDTAGSQFFIVVEDSFFLDRKYTVF
                     GEVARGLGVADKIANLPRNERDLPNDRIEMTVTILE"
     gene            complement(3494..3814)
                     /locus_tag="VFH05_02320"
     CDS             complement(3494..3814)
                     /locus_tag="VFH05_02320"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6677078.1"
                     /translation="MTVEKAKKVRKSRRVDLRCQLGFSSEEIEGDAHVTNISTGGCRA
                     ESEINVAEGLQFVVLLHLPGQTPLKIERASVRWVKGNSFGLSFILFLPSERAKLQHYL
                     EAIK"
     gene            complement(3827..4522)
                     /locus_tag="VFH05_02325"
     CDS             complement(3827..4522)
                     /locus_tag="VFH05_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677079.1"
                     /translation="MSACAYCRQRKGKRPCPALGAICSQCCGEHRLVRVSCPPDCLYL
                     DAGSDYQQKRLSEQFMPVRREFYREISEVGGEQAVALYNLIEVVMFSYFHGRRDGLDA
                     EVVTAIQALRRTLSPLHVPSAPMPVFAEHLKKEYETFKKQNPQQIADSSTAPDILDRA
                     IRFVSGFSGNDFQSRRFLSGLIGYVKMYHPEIAAHLTKQHQAGHIVLPGQSFMPPAAS
                     EPHIHGPGCRHHH"
     gene            complement(4519..5445)
                     /locus_tag="VFH05_02330"
     CDS             complement(4519..5445)
                     /locus_tag="VFH05_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677080.1"
                     /translation="MTPSAFGQITVTNLQTPYSFVTGASGKDYFISSVNGEPDTADNN
                     GFITKLDGSGTLLHLKFIQGGADGVTLHAPKGMALVDETLYVADLDTLRAFDARNGKP
                     VASIHVVDGKALVHLTDVAYDGVGLLYCSDQHANRIYRVELPSQKVTLLVADPALAGP
                     AGLAVHPKSGELIAVSWNKGKIFAISPTGTVSELFSNGFFSSRFSNLRGVDFDRWGNM
                     YVSDFTTGKVWRMTWDKRFQVIAEYLPTPGDLSIDRTNNLILVPYEFAHAAEMNGLEA
                     PADGKPKSEKRTLADYGFIPPPPKPAEGAPRK"
     gene            5768..6628
                     /gene="folD"
                     /locus_tag="VFH05_02335"
     CDS             5768..6628
                     /gene="folD"
                     /locus_tag="VFH05_02335"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET6677081.1"
                     /translation="MAARLIDGKALAQQVRESLVGASAAVLAKTGMKPGLATILVGDD
                     PASHVYVKNKQKACELAGIYVDDHKLSAHTTQAELLALIEKKNADQKIHGILVQLPLP
                     KHIDSRVILEAVSPDKDADGFHPYNFGRLVEGHPVFEACTPKGVIKMIESTGVPIEGK
                     RAVVVGRSNIVGKPLALMLLQRHATVTICHSKTRDLPAVCREAELLLVAIGKAKFVTA
                     DMVREGAVVIDVGTNKLPDGKLCGDVDYESVSQKAAWISPVPGGVGPMTIAMLLDNTV
                     ESAKRMAGMK"
     gene            6830..7717
                     /locus_tag="VFH05_02340"
     CDS             6830..7717
                     /locus_tag="VFH05_02340"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET6677082.1"
                     /translation="MSQEPKRLKDVLNQGQFAVTIEYNPPKGTNINGVLDNAKQLLGR
                     VHGVNVTDNTAAVMRAGSLPVCRLLYELGHDPVMQLTCRDRNRIAMQSDLLGAHMLGI
                     RNILCLTGDYPTVGDHKDAKPVYDLDSVQVMGLVQGLNNGRDLVGNKLDGSTAFTIGA
                     AVTPEAELVGPVLAKFEVKVKAGAQFFQTQAIYNTDQFASFMKAVRPFKVKVLAGILL
                     LRNAKMAEFMNANIPGMSVPQAMIDELRAAGDKSEDVGVEIAVRSIQAVRPHCDGVHI
                     MAIKGTHRLGEIITKAQLI"
     gene            7714..8505
                     /gene="panB"
                     /locus_tag="VFH05_02345"
     CDS             7714..8505
                     /gene="panB"
                     /locus_tag="VFH05_02345"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET6677083.1"
                     /translation="MMTVPDLQRYKRDKKKVIVVTAYDALFTRLLEQAGIEVILVGDS
                     LGVVVQGKPNTLAVTMEDMLYHTKLVAGTAQKSLVIGDMPFMSYQASKEDALRNAGRF
                     LQAGAHAIKLEGGAAMVDRVAAMTEVGIPVIGHLGMTPQSVNQYGGYKVQGKGKDQAQ
                     MLLSDAKALEAAGAVAVVLEAMPASLAQNITEEVAIPTIGIGAGPHCDGQVLVLYDLL
                     GMFDEFIPKFVKPYAHLKADALQALRRYKEEVEEGKFPSDAESYQ"
     gene            complement(8562..8942)
                     /locus_tag="VFH05_02350"
     CDS             complement(8562..8942)
                     /locus_tag="VFH05_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET6677084.1"
                     /translation="MEEVKAVLPHPEQWRGADEYLYGIDLYNYAYWWECHEVLERLWH
                     AAGRKTEQANFFRALIQLAAANLKHFLGDRHATSNLLAAGLARLETIPVWYMGVDVAN
                     LIDHLRQHKQGPAPLIRMNQRTTS"
     gene            complement(9061..9870)
                     /locus_tag="VFH05_02355"
     CDS             complement(9061..9870)
                     /locus_tag="VFH05_02355"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HET6677085.1"
                     /translation="MATYAIGDIQGCYKSLQRLLDRIAFAPRSDTLWLVGDLVNRGPD
                     SLLTLRFLQSLGSSVRPVLGNHDLFLLAAAEGIVTLRPKDTIQDVLNAEDCSDLINWL
                     RRQPLQQREGSFFMVHAGLHPRWTIEQAMSLAKEVEGVLAGPDYKTLLQELFHGPTRQ
                     WDPAATGMERLVSIARVFTRLRTISLTGEISSFSGPPEDAPPGFMPWFRIPDRRSNEG
                     IIITGHWAALGLHIEPNLLAIDSGCVWGRQLTAVRLEDRAVYQVEYADRRD"
     gene            complement(9971..10366)
                     /gene="queF"
                     /locus_tag="VFH05_02360"
     CDS             complement(9971..10366)
                     /gene="queF"
                     /locus_tag="VFH05_02360"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET6677086.1"
                     /translation="MRKKKTSLGYSERHAKSGITASLPDIETFPNQYKGYEITIEIPE
                     YTAICPKTGLPDFGTITVHYMPDRECLELKSLKMYIHAYRNIGIFYENSVNRILQDIA
                     RACRPVWARVTGTFAARGGLRSVIEARYP"
     gene            complement(10411..10659)
                     /locus_tag="VFH05_02365"
     CDS             complement(10411..10659)
                     /locus_tag="VFH05_02365"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET6677087.1"
                     /translation="MPIRVVIAGEDEEALHVGGVHKRPPIPDNSVKAECPKFMAHGPC
                     GGVRKGGFCEVYPEMTCPWITMFIELEKIGHTDWMKQV"
     gene            11045..>11697
                     /locus_tag="VFH05_02370"
     CDS             11045..>11697
                     /locus_tag="VFH05_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6677088.1"
                     /translation="MSLDYVKFSSGFEKFMPKEYRDMVEHGPFGKKVSVSQMGSFKEV
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTNRGAVLKMAPLGKKFEKMDMVQMAKVAGCAYVATVVPNNPRRVESV"
BASE COUNT         2618 a         3148 c         3451 g         2480 t
ORIGIN      
        1 tatcgccgag caactcgggg ttgaggaaga cgaagtgacg tcggaagcca gtttcgtcga
       61 agacctgggt gccgattccc tggataccgt cgaactcgtc atggcgctgg aagaggaatt
      121 tggaattgaa attccggacg aggacgcgga aaagattctg accgtcggca aggccttgga
      181 ctatatcaag gaaaaatcgt aaaggctgaa cgtgaagagc gacggtgcga agagacgcgt
      241 tgttgtcacc ggtctcgggg tgatcacccc tctcggcacc ggcgtggaca aaacgtggaa
      301 agcgatctgt tccggccagt cagggatcgg ccggatcacc aagttcgacc ccgccggcta
      361 tgacgcccaa atcgccggtg aggtcaaaga tttcgatccc gctcaattca ttgaaaaaaa
      421 agacatcaag aaaatggatg ccttcatcca ctatgcggtg gccgcagctc aattggccgc
      481 ggatgatgcc ggactcaagg tcttaccaga agaggcgacg aaagtcgggg tctacatcgg
      541 ctcagggatc ggggggctgg gatccatcga gcactatcat accgtgctca aagacaaagg
      601 gcctggccgg gtctcgcctt tttttatccc gatgaccatc atcaacctcg cttccggaca
      661 ggtggcgata cggttgggcg ccaaaggacc caattcgtgt gcggttacgg cgtgcgcgac
      721 agggaatcat tgcatcggcg atgcgttccg gcttatccag cgcggcgagg cggacgtgat
      781 ggtggccggc ggtgcagagg ccgcgatcac gacactcgga gtcgcgggct ttgccgcgtc
      841 aaaagcgctc tcgttccgaa atgacgagcc gacaaaggcc agtcgtccgt ttgataaaga
      901 ccgtgacgga ttcgtcctcg gtgaaggcgc cggcgtcatc gtgcttgaag agctggacca
      961 tgctcgcctg cggggcgtcc gcatctatgc cgaggtcgtg ggttacggca tgaacagcga
     1021 tgcctatcac atcaccgctc cttccgaaga aggtgagggc gccgtgcggt gtatggaatt
     1081 ggcgctggcc gatgccaata tggggaaaga tcaggtggga tacatcaatg cccacggcac
     1141 atcgacgatg gccgatgtca ttgagaccaa ggcgatcaag caagtctttg gtgaacgggc
     1201 cttgcgcatc ccggtgagtt cgacgaagtc gatgacaggg catttgctgg gcgccgccgg
     1261 cgggatcgaa gcggtgttca gcattctggc cattcaccac ggtattctcc cgccgaccat
     1321 taacctcgac cacccggatc cggaatgcga tctggattat gtcccccaca aggccagacc
     1381 tgcgacagtc aatgtggcgc tctcgaattc gttcggattc ggcggggtga atgcctgcct
     1441 gctcttcaaa aagcccgaag cctgacgcat tcacgcctcc cggcgtctgc ctagttgtat
     1501 gacaacggct cccccgctgg attcgcttca agccgctctt gcctatcggt tcaccaatcc
     1561 cgtgttgctt caagaggcct tgacgcacaa gtcatttatc aacgaacgga agaccacagg
     1621 aggctcgcac aacgagcggc tggaatttgt gggggacgcc gtgttgtcgc ttgtcgtcag
     1681 cgagcaactc gcggcgttgc tgccgcaatc atccgagggc gctctttcga agcataaggc
     1741 tcgactggtc agtgaatcga tgctcgctca tgtcgccaga cacctcaaag tgggagactg
     1801 tctgcggctt ggccgcggcg aggagttgtc tcgagggcgc gaaaaagatt cgctgcttgc
     1861 tgatgcgctg gaagccctca tcgccgcggt gtatctcgat ggcgggctcg aatcatcccg
     1921 ccgcgtcgtg atcggattgt tggagcgcga attgaggcaa gtggttactc aacagcatcg
     1981 tccgggtgag gacgattaca agacgcaggt ccaggaatgg tgtcagcgac gcttcgacag
     2041 tctcccctct tatacagtcg ttcgtgagtc gggaccagat cacgacaaaa tcttcgaagt
     2101 ggaagttgcc gtcaacggga aagtcgtcgg cagaggaacc ggacgcagca agaaggaagc
     2161 cgagcagtct gcagccaagc aagcattgca tgaagcttga cggtcggcgg ggttgttatg
     2221 ataaaggcca catagaatca tgatggaggt acgcacatgc agagactggt gatgagactg
     2281 gcgatggcag tgagcgttat gacaacagcc tggactcttg ttggacctgc cggagtcgcc
     2341 ggcgagacgg gcgcaggcaa agcgccaaaa gccgtcatca agaccaaatt cggagacatg
     2401 gaaatcgcat ttttccccga caaggcccca aaccatgttc agaatttcat caagctggct
     2461 aagtcagggt attacaacgg aaccattttt catcgcgtga ttccaggttt catgattcag
     2521 gggggtgatc cgaacacgaa agatccgaag aagcccgaga cctacggtat gggtggaccg
     2581 agtgacaagc tcaaagcgga attcaatgac acaccgcatc gccgcgggat tgtctcgatg
     2641 gcgcgcagta acgaccccaa tagtgcgggc tcgcagttct tcatcgtcat caaagattcc
     2701 aactttctgg acgggcaata cacggcattc ggcgaagtcg tgaaaggaat ggaagtggcc
     2761 gataagatcg tcaatctccc aagaaatgcg cgcgatctcc ccaacgaacg cgccgagatg
     2821 accgtcgtcg tcgaatagat ggtccgcctg cagaaaggag acggctagtg gggaaaagaa
     2881 gcggtctcat gctcatcacc gcgatgatca tggccatgaa tgtctctgcc ggctgctccg
     2941 gcaaaagcga cctcaaggcg gccgtaccgc ctccggaccc tcgcccgaga gccatcatca
     3001 aaactcggtt cggagaaatg gaactcaagt tatatccaga tctcgcccct aaccacgtcg
     3061 agaatttcat caaactggcg aagtccggtt tctataacgg gacaattttc catcgcgtca
     3121 ttccaggctt tatgattcag ggcggcgacc ccaataccaa gtactcgctc agaaaggaca
     3181 cgttcggcca ggggggaccc aaggacgaaa agggtgatcc gattttgttg aaggctgaat
     3241 tcagcgatac gccgcacaag cgaggcatcg tgtcgatggc gcgagccggg gagccggata
     3301 cggccgggtc tcagtttttt atcgtcgttg aggattcgtt ctttctcgac cgtaagtaca
     3361 cggtctttgg cgaagtggcg aggggtttag gtgtggctga caagatcgcc aatctgccga
     3421 gaaatgaacg ggatctcccg aacgatcgga ttgaaatgac cgtgacgatt ctagagtgaa
     3481 tggatgatcg gggttacttg atcgcttcca gataatgttg gagtttcgcc cgttcagatg
     3541 ggaggaagag gataaagctc aatccgaagc tgttgccttt gacccagcgt acggaggcac
     3601 gctcaatctt aagaggagtt tgtccaggaa gatgaagcaa gacgacaaat tgtaatccct
     3661 ccgcgacgtt gatttcagac tccgcccgac acccgccagt ggagatattc gtcacatgcg
     3721 cgtctccttc gatttcttcg gaggagaatc caagttggca acgcaggtcc acgcgacggg
     3781 attttcgaac tttcttcgct ttttcgacgg tcatcgcggt ctacctctag tgatggtgcc
     3841 tgcatcccgg tccatgaata tgcggttcac tcgctgccgg cggcatgaac gactgaccgg
     3901 gaaggacgat atggccggcc tgatgctgct tggttaagtg tgccgcgatc tccgggtggt
     3961 acattttcac gtagccgatt agaccactta aaaatcgacg cgattggaag tcatttccgg
     4021 agaacccgga cacaaatcga atggcccgat caaggatatc gggagcggtc gagctgtcgg
     4081 cgatttgctg agggttctgt ttcttaaagg tctcgtactc tttcttgaga tgttccgcga
     4141 agacgggcat cggcgccgag ggtacatgca gtggactcag cgtgcgtcgc agcgcttgga
     4201 tggcggtcac gacttcggcg tccaggccgt ctctacggcc gtgaaagtaa ctgaacatga
     4261 cgacttctat cagattgtag agggccaccg cctgttcgcc tcccacttcg ctgatctccc
     4321 tgtaaaactc ccgtcggacc ggcatgaact gttcgctcag tcgtttctgt tgatagtcgc
     4381 tgcccgcgtc aagatacaga cagtctggcg ggcatgagac ccgcacgaga cggtgctccc
     4441 cgcagcactg actgcagatg gcccctaagg cgggacaagg ccgcttgcct ttccgctgtc
     4501 ggcaataggc gcaagcgctc atttccttgg cgctccttcc gccggctttg gcggcggagg
     4561 aatgaaaccg taatccgcca gcgtcctctt ttcagattta ggcttgccgt cagcgggggc
     4621 ttccagacca ttcatctcgg cggcatgggc gaactcgtaa gggaccagaa tcagattgtt
     4681 cgtgcgatca atgctgaggt cgccgggcgt aggcagatac tcggcaatga cctgaaaccg
     4741 tttgtcccag gtcatccgcc agactttgcc ggtcgtgaag tcggacacgt acatattgcc
     4801 ccaccgatcg aagtccaccc cacgcaggtt cgagaagcgg ctggagaaaa agccgttgga
     4861 aaagagctcg gagactgtcc cggtcgggct gatcgcaaat attttgcctt tgttccaact
     4921 gacggcaatg agttcgccgg attttggatg taccgccaac ccagcgggcc cggccaatgc
     4981 aggatcggcc accaacaggg tgaccttctg cgagggcaac tcgacgcgat agattcgatt
     5041 ggcgtgctga tccgagcagt acaacagccc cacgccgtcg taagccacgt cggtcaaatg
     5101 aaccagtgcc ttcccatcaa ccacatggat ggaggcaacc ggtttcccat tgcgtgcgtc
     5161 gaatgcacgc aacgtgtcga gatcggccac ataaagcgtc tcgtccacca gcgccattcc
     5221 ctttggcgcg tgtaaggtga cgccatccgc cccgccctgg ataaatttga gatgtaagag
     5281 agtcccgctg ccatcgagtt tggtaataaa gccgttgttg tcggcggtgt ccggttcgcc
     5341 attcacgctc gaaataaaat agtcttttcc cgaagcgccg gtcacaaagc tatagggggt
     5401 ttgcaagttc gtgacggtga tctgcccgaa tgcagaaggg gtcatggccg ccaggcacag
     5461 gcctgcacat ccgatgacaa catagaacag gtaacggatc atatgggtta ggtgggggga
     5521 cgtttatggt gccgttgcga tggacatcgg aatcatcgta gccgcgacct ggagaacctg
     5581 tcaagaaatc ggcggaaaag ccaactgcca tgctgtgcag aggcgcgttc cgcccatacg
     5641 ttgacttgcc taaaattgcc ctgttaaggt gccgtgctct taagcaagtc actggtggac
     5701 caggctcaag cggggctccg atccaccgcg caccttctca ctccggattg tgcaatggga
     5761 ggacgttgtg gcagctcgat tgattgacgg gaaagcattg gcccagcagg tgcgcgaaag
     5821 cctcgtcggg gcatctgcgg cggtgttggc aaagacgggg atgaagccgg gtctcgcgac
     5881 gattctcgtc ggagacgacc ccgcgtcaca cgtctacgtg aagaacaaac agaaggcctg
     5941 cgaactggca ggcatctacg tcgacgacca caaattgagc gctcatacca ctcaggcaga
     6001 actcctggcg ctcatcgaga agaaaaatgc cgatcagaag attcacggca ttctcgttca
     6061 gctgccgttg cccaaacaca tcgacagccg cgtgattttg gaagccgtct ctcctgataa
     6121 ggatgcagat gggtttcatc cctataattt cggccgactc gtggaaggcc atccggtgtt
     6181 tgaggcctgt acgccgaagg gcgtgatcaa aatgattgaa tccaccggcg tcccgatcga
     6241 aggcaagcgt gcggtggtcg tcgggcgcag caacatcgtg ggcaagccgc tcgcgctcat
     6301 gctgttacag cgtcacgcga ccgtcacgat ctgccattcc aaaaccaggg acctccctgc
     6361 ggtatgccgg gaagccgaac tcctgcttgt cgccatcggc aaggcgaaat ttgtcaccgc
     6421 cgatatggtc cgcgaaggcg cagtcgtgat cgacgtcgga acgaataaat tgccggatgg
     6481 caaactctgt ggcgatgtcg attacgagtc ggtcagccaa aaggccgctt ggattagtcc
     6541 cgttccgggc ggggtcggtc ccatgacgat tgcaatgttg ctggacaata cggtcgagtc
     6601 tgctaagaga atggctggga tgaagtaggc tggctcctta agacaacgtt gcgtgaagtg
     6661 gcgccgtgtt gcttccttgc tcaccgagtg cgcacgcagc aatagtcgga tttttagatc
     6721 gagggcggtg ctccctcgac gtgcgcagtg gaaccaatca cggcgccagt cctgagagag
     6781 aagtggagca ttgcagccag cctccgcgct ggtggcgaag gggaatagga tgagtcagga
     6841 gcctaagaga ctgaaagacg tcctgaacca gggacagttc gctgtcacga ttgaatacaa
     6901 ccccccgaag ggaacgaata tcaacggcgt gctagacaac gccaagcaat tgttgggtcg
     6961 cgtacacggc gtcaacgtga ccgacaacac cgccgcagtc atgcgggcgg gatctttacc
     7021 ggtgtgccga ttgctgtatg agttgggaca tgatcccgtc atgcagctga cctgccgaga
     7081 tcgaaaccgg atcgccatgc aatcggatct tttaggcgca cacatgctcg gcatccgcaa
     7141 cattctctgt ctcaccggcg actatccgac tgtcggcgac cacaaggacg cgaagcccgt
     7201 gtatgatctt gattcggtcc aggtgatggg gcttgtccaa gggttgaaca acgggcgcga
     7261 tctggtagga aacaagcttg acggctccac agccttcacc atcggggctg cggtgacgcc
     7321 ggaagccgag ctggtcggtc ctgtgttggc gaagttcgag gtcaaagtga aggcgggcgc
     7381 gcaatttttc caaacgcagg ccatatacaa caccgaccag ttcgcgagct tcatgaaagc
     7441 cgttcgtccg ttcaaggtca aagtgctggc cggaattctt ttgttgcgca acgccaaaat
     7501 ggcagaattc atgaatgcga acattccggg catgtcagtg ccgcaggcga tgatcgacga
     7561 attgcgtgcg gcgggagaca agtccgaaga cgtcggtgtg gaaatcgcag ttcgcagcat
     7621 tcaggccgtc cgtcctcatt gcgacggcgt gcatatcatg gcgatcaagg gcacgcatcg
     7681 gctcggagaa atcattacca aggctcaact catatgatga ccgttcccga tcttcagcgc
     7741 tataagcgcg acaagaaaaa agtgatcgtg gtgaccgcgt acgacgcgct ttttacccgg
     7801 ttgctcgagc aggcaggcat tgaggtaatt ctggtcggtg attccttggg cgtcgtcgtg
     7861 caaggcaaac ccaataccct tgccgtgacg atggaggaca tgctgtacca cactaaactt
     7921 gtcgccggga ccgcacagaa gtcgttggtg atcggagaca tgcccttcat gtcgtatcag
     7981 gccagcaagg aggatgccct ccgcaacgcg ggccgcttcc ttcaggccgg cgcccatgca
     8041 atcaaactgg aaggcggcgc tgcgatggtc gatcgggttg ccgcgatgac ggaggtcggg
     8101 attcctgtca ttggacatct cggcatgacc cctcaatccg tcaaccaata cggcggctac
     8161 aaagtgcagg gtaagggcaa ggatcaggca caaatgctct tatccgacgc caaagcgctt
     8221 gaagcagcgg gtgccgtcgc cgtcgtgctg gaggcgatgc ccgcatccct ggcgcagaac
     8281 atcaccgagg aggtggccat cccgaccatc ggtattggcg caggccctca ttgtgatggt
     8341 caagtgctgg tcctgtatga cctgctgggc atgtttgacg agttcattcc caagttcgtc
     8401 aagccttatg cccacctgaa agccgatgcg ttgcaagcac tccgccggta taaggaagag
     8461 gtggaagagg ggaaatttcc ctccgatgcc gagtcctatc agtaaacagg cgctgagggc
     8521 tgtcgacgct ccattcgctg tccctccttg tacgcagctt gtcacgacgt cgttctctga
     8581 ttcatgcgta tgagcggagc aggcccttgc ttgtgctgcc ggagatggtc gatgaggttc
     8641 gcgacgtcaa cacccatata ccagacagga atcgtctcca atcgcgcaag acctgctgcc
     8701 aacaaattgc ttgtcgcgtg acggtcgccg agaaagtgtt tgagattggc tgcagcgagt
     8761 tgaatcagtg cccggaaaaa attcgcctgt tcagtctttc ttccagcggc atgccatagg
     8821 cgctcgagga cttcgtgaca ttcccaccag taggcgtaat tgtagagatc gattccgtag
     8881 agatactcgt cggcccccct ccattgctcg gggtgcggca ggactgcctt gacctcttcc
     8941 accccatatg aatgtcctcg cgggtctcga cgaggatggg gtaaccggcc ggggataaat
     9001 cgatagaatg gaaatgggcg atgagagtac cggggccagg acggatccgg agctcggggc
     9061 tcaatcgcgt cgatctgcat actcgacctg atacacggcg cggtcctcca agcgcactgc
     9121 cgtaagttgt cttccccaca cgcacccgct gtcgatcgca agcaggttcg gttcgatatg
     9181 cagaccgaga gccgcccaat ggcctgtgat gataatccct tcgttgctcc gacgatcggg
     9241 tatgcggaac cagggcatga aacccggcgg ggcgtcctcc ggagggccgg aaaaactcga
     9301 aatttcaccg gtcaaggaaa tggttctgag ccgcgtgaac acacgcgcaa tactgaccag
     9361 gcgttccatt cccgtggctg ccggatccca ctgccgggtc ggaccatgga aaagttcctg
     9421 caacaacgtt ttgtagtccg ggccggccaa gacgccttcg acctccttgg ccaagctcat
     9481 cgcctgctct atcgtccatc gcgggtgcag tcccgcatgg accatgaaga aggatccttc
     9541 gcgctgctgc aagggctgcc gtctgagcca attgatgagg tcagagcagt cttcggcgtt
     9601 caagacatct tgaatggtgt ctttcgggcg gagtgtcaca atgccttctg cggcggccaa
     9661 taaaaataga tcgtggtttc ccagcacggg acgcaccgat gagccgagag attgaaggaa
     9721 gcgaagagtc agtagggagt ccggtcctcg attgaccaag tcccccacga gccaaagcgt
     9781 gtctgaccgt ggagcaaacg cgatccggtc gagcagacgc tggagagatt tgtagcagcc
     9841 ttgaatgtcg ccgattgcat aggtggccat gagtaccgaa gcgtcagtgt gcgatgggtc
     9901 agaaactggc aggacgttga acgctcatgt cggccctgcg gctacctgac gatctcgtgt
     9961 ggtttgtggt tcatggatag cgggcctcta tcacgctacg gagtccacct cgggccgcga
    10021 acgtgccggt gactcgcgcc caaacagggc ggcaggctct ggcaatatct tggagaatcc
    10081 gattgaccga gttctcataa aaaatgccga tgttccggta ggcatgaata tacattttta
    10141 gtgattttag ttcgaggcat tcacgatccg gcatgtaatg cacggttatc gtgccgaagt
    10201 ccgggagccc tgtttttgga caaatcgccg tgtactcagg gatctcgatc gtgatctcat
    10261 agcccttgta ctggttaggg aacgtttcga tatcagggag cgaagccgtg attccgcttt
    10321 tcgcatggcg ctcgctatag ccgaggctgg tcttcttctt tctcattctc atacggtaag
    10381 atgagcgatg cgaaggttaa gcccgaggtc tcatacctgc ttcatccagt ccgtgtgacc
    10441 gattttttcc agttcaataa acatggtgat ccatgggcag gtcatttccg gatagacttc
    10501 gcaaaagcca cctttccgga cacctccgca agggccgtga gccatgaatt tcgggcattc
    10561 ggccttcacc gagttatcgg ggatcggcgg tcgcttatgg acgcccccga cgtgcaaggc
    10621 ctcctcgtct tcgccggcta ttacgacgcg tatcggcata acttggctag tgtagacaga
    10681 ttttttgcca agggcaacgt acatctgcct tgtgattcgt atcggttgca ttttaggagc
    10741 cgtcaaggtt agggcgaatg gcctatcgca ttaaggaagg gtaggccttc tttcggacag
    10801 ggtgggcctt ctgcaaagtt gcattgaata tcaggaagtt ataaaatttt cgaggtgagc
    10861 cgataatccc cgttgcgaga acatataaat tgacagtgtt ttttgagctt tgctaggatg
    10921 gccgcgacgc gaacatgagc cggtgaacgt gtcgcttgtc cgttctcttt cccgctgatc
    10981 gtcttacttt acactgaatg aatggcgacc tagtccgtcg cggtgacata aagggaggtg
    11041 tccgatgagt ctcgattatg taaagttttc aagcggcttt gaaaagttca tgccgaaaga
    11101 atatcgggac atggtcgaac atggaccctt cggaaagaag gtgtccgtct cgcaaatggg
    11161 cagcttcaaa gaagtcctcg aagaacatcc gatgtgcgct ggctgcgcca tgacgctgtt
    11221 tattcgcctc gccatgattg cgtttcccaa tcctgaggac accattaccg tcggtaccgc
    11281 cggatgtggc cgcttggcga tttctcaagc cgccatcccc ttcgtgtacg gcaactacgg
    11341 cgaccaaaac ggtgtagcca gcggactttc tcgcggtctt cgccttcgct tcggcgacaa
    11401 gccgaaagac gtggtcgtca tggccggtga tggcggcacc gccgacatcg gctttcagca
    11461 agtcctccat tcctggttcc ggaaggagcg cttcacgacg atcatgctgg acaacgaagt
    11521 gtacggtaac accggcgggc aggaaagcgg catgacgaac cgcggggcag tgttgaagat
    11581 ggcgccgctc ggcaaaaagt tcgagaagat ggacatggtg caaatggcaa aggttgccgg
    11641 atgcgcgtat gtcgcgacgg tcgtgccgaa caacccgcgc cgcgtcgaga gcgtcat
//
LOCUS       DASSCQ010000036         5539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_709020, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000036 DASSCQ010000000
VERSION     DASSCQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709020"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VFH05_02375"
     CDS             <1..1707
                     /locus_tag="VFH05_02375"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HET6677089.1"
                     /translation="EAKLVLRGRYLTKNDHGTVTETVPELFWRIATDIAQAEQLYPGP
                     TRLVHAARRFYESMARLEFLPNSPTLMNAGRPLQQLAACFVLPVEDSLSSILEAVKQQ
                     ALIHQSGGGTGFSFSRLRPHDDLVSSTQGTASGPVSFMRIFNMATDVIKQGGTRRGAN
                     MGILRVDHPDILEFIGLKQDQSAMNNFNLSVGITSEFMRALARRRRYPLINPRTGKPT
                     KRLPATMVFDRLVQAAWATGEPGVIFLDHINADNPTPQLGDIEATNPCGEQPLLAYES
                     CTLGSINVSKFVRPGRPDPVIDYERLATAITLAVRFLDNVIDRNHYPLKEIEIATLRT
                     RKIGLGIMGFADLLIALNIPYDSEGAIQHATSLMSFVRAQAHEASHRLGAQRGWFPAH
                     RSSRLQSRHMQRRNATVTTIAPTGTISLIAGCSAGIEPLYGVRVLRRAMDGLELTMVH
                     PAFARMIRARGLDLDQIQTDLGASPSIQHVTRIPKDIRRLFVTAHDVAPRQHVRMQAA
                     FQRSSDSGVSKTINLPSTATESDVAEAFLLAHELKCKGLTVFRSGSREAQVLSCSPSL
                     PC"
     gene            1821..2549
                     /locus_tag="VFH05_02380"
     CDS             1821..2549
                     /locus_tag="VFH05_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677090.1"
                     /translation="MAELARSLTVVGNPLPWSLMFARQPEPDLEFTEEELDQTTATRP
                     MAPMKPGKKSGGGRPMLWIFLLLLVGGIAYLSMEPEQVADWLAPFTGDSTPEPAPIAS
                     RPKPAPPAPAATAPVTPPATSEGMPAPTAPPAPSSADSPGQPTPPSSAPPALTPPAGA
                     VVSSPMFAEGQKVTAIGNPTIPAETIPLSLDPAGAKPGPVVRPGVTLSILDGDLQPSG
                     WVYSVRTDDGVRGWIAEKRLRLKF"
     gene            2664..3353
                     /locus_tag="VFH05_02385"
     CDS             2664..3353
                     /locus_tag="VFH05_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET6677091.1"
                     /translation="MSTLRALIFDFDGVIANTEPLHFAGLHQTLKEIGIDLTEADYYA
                     NYLGYDDHGCFVAALTAHHRPVDSGTIAQLMKRKASAYLESVKRHQVIFAGVPEFIRE
                     AARLYPLAIASGALRHEIEYILEAAGLRKEFFHITSAEDVLRGKPDPQPFMLAMKGLQ
                     QREPGLTPGSCLVIEDSLPGVRGAKAAGMKVLAVANTHTVQDLHEADAITPSVQETNL
                     TDLRRRLWQEP"
     gene            3367..4305
                     /locus_tag="VFH05_02390"
     CDS             3367..4305
                     /locus_tag="VFH05_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HET6677092.1"
                     /translation="MRICSLVPGATEVVALLGLSHDLVGISHECDYPETVRHAPVVVQ
                     PTFDSAVMDSAAIDRQVRAQASSGQKLYEVNGQALIDARPDVILTQDVCHVCAVTAHQ
                     LEQTIATLPSRPQVLTLDSRSLKAVIDDVERIGQAVGEPARGRVLAESLSDRLRTVEG
                     KHRSLPRLRVLCLEWLSPLYVGGHWVPEMVEIAGGQDVLGCAGQPSRQVSWQEVHAAA
                     PQVVVLMPCGFSIERTVAELRHLCRTDHDWSQQLSLWPRTYIVDASSYFSRPGPRLVD
                     GVELLGGIISGAVAAGCNSPMVRDVSGSLALLGSSR"
     gene            4308..5168
                     /gene="hpnD"
                     /locus_tag="VFH05_02395"
     CDS             4308..5168
                     /gene="hpnD"
                     /locus_tag="VFH05_02395"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HET6677093.1"
                     /translation="MTTAEAQAFCTDLTKKSGSNFYYSFLFLPKARRNAMYTVYAFCK
                     AVDSAVDEPPAGSNPKEELRRWRSELELVYSGRPTWPLMISLAHHVKQLSIPKAYFDE
                     LIKGVEMDLSRTRYRTFEELSLYCYRVASIVGLICLHIFGPTSPHAQDYAVDLGMAFQ
                     LTNILRDLSADADQGRIYLPQEDLEKFGYSETDLLKHIESPALHDLIRFEATRARTYY
                     TKAQEALSCLPKKDRRALTVAEIMRAVYSRILDRIEQADHTVFGPRVRLSTSNRLALA
                     SGVWLRSKLS"
     gene            5165..>5539
                     /locus_tag="VFH05_02400"
     CDS             5165..>5539
                     /locus_tag="VFH05_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6677094.1"
                     /translation="MTPHGRPSVIIIGGGPAGMTAAYRLTAQGCRVTLLTSSSILAER
                     FGQAGSQPTAILGCHHATWALLQSLGIHRTDPLFTESALEFVLSDGRVARYPKTFFPT
                     PLKQLLTIGRFAGLSPAERWKLL"
BASE COUNT         1095 a         1751 c         1539 g         1154 t
ORIGIN      
        1 gaagcgaagc tggtgcttcg tggacgttat ttgacgaaga acgaccacgg cacggtcact
       61 gagaccgttc ctgagttgtt ctggcgcatc gccacagaca tcgcgcaggc tgagcagctc
      121 tatcccggcc ctaccagact cgtgcatgcg gcgcgccggt tttacgaatc catggcccgg
      181 ctggaatttc ttcccaactc gcccacgctc atgaatgccg gacgaccact gcagcaattg
      241 gccgcctgtt tcgttctgcc ggtcgaggac tccttgagtt caattttgga ggcggtcaaa
      301 caacaagcgt tgatccacca atcaggcggt ggcaccggtt tctccttcag ccgccttcga
      361 ccgcacgacg atctggtttc ctcgacccag gggactgcct cggggcccgt gtcattcatg
      421 cgcatcttta atatggccac agacgtcatc aaacagggcg gcacgcgtcg cggcgcgaac
      481 atgggcatcc ttcgggtcga tcacccggat attttggaat tcatcgggtt gaagcaagat
      541 cagtccgcaa tgaacaactt caatttgtcc gtgggcatca cgtcagagtt catgcgtgcg
      601 ctggcacgtc gtcgacgcta tccgctcatc aatccgcgga ccggaaaacc aaccaagcga
      661 ttgcccgcca cgatggtgtt cgaccggctg gttcaggctg cctgggcgac gggagagccg
      721 ggcgtcattt tcttggacca catcaatgcc gacaatccaa caccccagtt gggcgacatc
      781 gaagccacca atccctgcgg agagcagccg ctcctggcgt atgaatcctg caccctcggt
      841 tcgatcaacg tgtccaagtt cgtccggcct ggccgcccgg accctgtcat cgactatgag
      901 aggttggcga cggccattac cctcgccgtc cgtttcctgg acaatgtcat cgaccggaac
      961 cattacccgc tgaaggagat tgaaattgcg acgctccgga cgagaaaaat cgggctcggg
     1021 atcatgggtt tcgccgacct cctcatcgcc ttgaacattc cctatgacag cgagggcgcg
     1081 atccaacacg ccacgtcgct gatgtcattc gtccgcgcac aggcccacga ggcttctcat
     1141 agactcggcg ctcaacgggg ctggtttccg gcgcatcgca gcagtcgcct gcagagccgc
     1201 cacatgcaac ggcgcaacgc gactgtcacg acgattgcgc caaccggcac gatcagtttg
     1261 atagccggat gttccgccgg tattgaaccg ctctacggcg tgcgcgtgct tcgccgggcg
     1321 atggatggcc tcgaactgac gatggtgcac cccgcctttg cgcggatgat cagggcccgt
     1381 ggcctcgacc tcgatcagat acagaccgat ctcggcgcaa gcccgtccat tcaacatgtc
     1441 acacgtattc ccaaagacat ccgccggctg ttcgtgacgg ctcatgatgt ggcccctcgg
     1501 cagcacgtgc gcatgcaggc agcctttcaa cgctcgagcg atagcggcgt ctcgaagacg
     1561 atcaaccttc cctctacggc aactgagtcc gatgtcgccg aggcgtttct gttggcccat
     1621 gagctcaagt gcaaaggctt gaccgtcttt cgcagcggta gccgagaagc ccaagtcctt
     1681 tcttgctcgc cctctctgcc ctgctgatca gaccgggcag caatcgatcg taaaattgac
     1741 tctccggagt gatggtgata agtatttctg caaattttcc gcatgagtcc gcctcgttcg
     1801 tcgcagcttg gaggtgtgtg atggcagagc tcgcaaggag cctgacagtg gtcggcaatc
     1861 cgctcccatg gtctctcatg ttcgcccgtc aaccggagcc ggacttggag tttacggagg
     1921 aagaactgga tcagaccacc gccaccaggc caatggctcc tatgaagccg gggaagaagt
     1981 caggcggcgg caggccgatg ctgtggatat ttctgcttct gttggtcggt gggatcgcct
     2041 acctctcaat ggaacccgaa caagtagccg actggctcgc tccatttacc ggagacagca
     2101 caccagagcc tgctcccatt gccagtcgtc caaaacctgc cccgccggct cccgccgcca
     2161 cggcaccggt gactccgccg gcaacatcgg aagggatgcc tgcaccaaca gccccacccg
     2221 ctccgtcgag cgccgattcc cccggtcaac cgacgcctcc ttcatcagcc ccaccggctc
     2281 tgaccccacc cgctggcgcc gtcgtctctt ctcctatgtt tgcggaggga caaaaagtca
     2341 ccgccatcgg caacccgacc attcctgctg aaacgattcc gctgtcattg gatcccgctg
     2401 gcgcaaaacc gggccctgtt gttcgccccg gtgtcacgtt gagcatcctt gatggagacc
     2461 tgcagccgag cgggtgggtc tactccgttc gcacggacga cggcgtcaga ggatggattg
     2521 ccgaaaagcg cttacgtttg aagttctagc ctcctttttc tgtaccaggt cacgaactag
     2581 gacgagagcg attcttcggc cttccgttcg ttgccctgtg atataattcg cgccggacac
     2641 ctccagcacg attctcccat cctatgtcga cactccgggc actcatcttc gatttcgacg
     2701 gcgtcattgc caataccgag ccgttgcact ttgccgggct gcaccaaact ctgaaggaaa
     2761 tcggcatcga cctgacggaa gccgactact acgccaacta tctcgggtac gatgaccatg
     2821 ggtgctttgt cgccgccctg actgcacatc accgccccgt cgattccggc accatcgcgc
     2881 aactcatgaa gcggaaagcc tcggcctatc tcgagtccgt caagcgtcat caggtcatct
     2941 ttgcgggcgt tccggagttc atacgggagg cggcacggct ctatcctttg gcgattgcgt
     3001 ccggcgcctt gcggcatgaa attgaatata ttcttgaggc cgcaggactc cgaaaagaat
     3061 ttttccatat caccagcgca gaagatgtgc tgcggggtaa gccagatccc cagccgttca
     3121 tgctggcgat gaaggggctg caacagcggg aaccgggcct gactcccggc tcttgcctcg
     3181 tcatcgagga ttcgctcccc ggtgtgcgcg gcgcaaaggc tgccggcatg aaggtgctgg
     3241 ccgtggccaa tacgcacacc gtccaagact tgcatgaggc cgatgccatc acccccagcg
     3301 tgcaagagac gaatttgacc gacctgcgca ggcgtctgtg gcaggaaccc tgacggtaga
     3361 acagacatga gaatatgctc gctggtccct ggtgcgacgg aggtcgtggc gttgctgggc
     3421 ttgtcgcatg atctcgtcgg gattagccat gaatgcgact atccggaaac ggttcggcac
     3481 gcgcctgttg ttgtgcaacc gaccttcgat tccgccgtca tggacagcgc cgccattgac
     3541 cgtcaggtcc gcgcacaggc gtcgtcgggt cagaagttgt atgaggtgaa cgggcaagcc
     3601 ttgatcgatg cacggcccga cgtcatcctg acgcaagacg tctgtcatgt ctgtgccgtg
     3661 accgcgcatc aactcgaaca gaccattgcc acgcttccat cacggccgca ggtccttacc
     3721 cttgattcgc ggtcactgaa ggcggtcatc gatgatgtcg aacggatcgg ccaggccgtg
     3781 ggtgaaccgg ccagaggacg tgttctcgcc gaatccctgt ccgataggct gaggacggtt
     3841 gaaggcaagc accgcagcct gccccgcctg cgagtcctgt gtctcgagtg gctgagcccc
     3901 ttgtatgtcg gcggtcattg ggtaccggag atggtggaga ttgccggagg ccaggacgtc
     3961 ctaggctgtg ctggtcagcc ctcccgccag gtttcctggc aagaagttca cgccgccgct
     4021 ccgcaggttg tcgtgctcat gccttgcgga ttctcgatcg agcgcacggt cgcggagctc
     4081 cgccacctgt gccggactga tcatgactgg tcgcaacaat tgagcctatg gccaagaacc
     4141 tacattgtgg atgcgagctc gtatttcagc cgacccggtc ctcgcctcgt cgacggcgtc
     4201 gagttactcg gcggcattat ttcgggagcc gtagccgccg gctgcaattc ccccatggta
     4261 cgtgacgtga gcggatcgct cgctctccta gggtcatcgc gatgacgatg acaacagcag
     4321 aagcacaggc gttttgcacc gatctcacga agaagagtgg aagcaacttc tattactctt
     4381 ttctctttct cccaaaagct cggcggaacg ccatgtatac ggtgtacgcc ttttgcaaag
     4441 cagtggacag cgccgtcgat gaaccgccag ccggcagcaa tcccaaggaa gaacttcgtc
     4501 gctggagaag cgagctcgag ctcgtctata gcggacgacc gacgtggccg ctcatgatca
     4561 gtctggcgca tcacgtcaaa caactttcca ttcccaaagc ctacttcgac gaactcatca
     4621 aaggcgtcga aatggatctc tctagaacgc gctaccggac cttcgaggag ctgtcgctgt
     4681 attgttaccg agtcgcctcg atcgtcggtt tgatatgcct gcacatcttc ggtccaacct
     4741 ctccgcatgc tcaggattat gccgttgacc tcggcatggc ctttcagctc accaatattc
     4801 tccgagactt gtcggcagac gcggaccagg gacgaatcta ccttccccag gaggatctgg
     4861 aaaaattcgg ctacagcgaa accgatctgc ttaagcacat tgaaagtccc gccctgcacg
     4921 atctcatccg gttcgaagcc acgcgagcaa ggacttacta cacgaaggcg caggaggcac
     4981 tgtcttgcct gcccaaaaag gaccgtcgtg cactgacagt ggccgaaatc atgagggccg
     5041 tatactcccg catcctcgat cgtattgaac aagcagacca caccgtcttc gggcctcggg
     5101 tacgcttgtc tacgtccaat cggctcgcgc tcgcgtccgg tgtgtggctc cgctcgaaac
     5161 tctcgtgacg ccgcacggcc gcccatctgt catcatcatc ggcggtggac cagccggcat
     5221 gacggccgcc tatcggctga cggcacaggg gtgccgcgtc accctcctca cttcatcatc
     5281 gatactggct gaacgattcg ggcaggcagg gagtcagccc actgccatcc tcggctgtca
     5341 tcacgctacc tgggcgctgt tgcagtcatt gggcatccac cgaaccgacc ctctgtttac
     5401 cgaatccgcc ttagagttcg ttctgtccga tggccgggtg gctcgctatc ccaagacctt
     5461 tttccccaca ccgctgaagc aactcctcac gatcggacgt tttgcaggac tatcgccggc
     5521 cgagcgttgg aaactcctg
//
LOCUS       DASSCQ010000037        16919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_479768, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000037 DASSCQ010000000
VERSION     DASSCQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 16919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16919
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479768"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1750
                     /gene="ilvD"
                     /locus_tag="VFH05_02405"
     CDS             77..1750
                     /gene="ilvD"
                     /locus_tag="VFH05_02405"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET6677095.1"
                     /translation="MHHAPRLNSRDLLVGPGRAPARAMLKAVGFTDEDLSRPLIGVAN
                     TWIEVMPCNFHLRRLSDRVKAGIRAAGGTPIEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDGVVALSGCDKTIPGTVMALARLNVPSLMLYGGSIMPGQFQGHD
                     VTIQDVFEAVGKHASGKMTNAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGMSPM
                     GRNGVPAMDQRKDDVAFECGKMVMELLKRDLRPRQIITRKSLENAIAAVATTGGSTNA
                     VLHLLAIAYEAGIKLNIDDFDRINRKVPLLADLKPGGKFAAADLYAAGGTTLVAKRLV
                     DAGLLHANQPTVSGRTIGEEAKDATETAGQRVLRPLSDPIKATGGLVILKGNLAPEGC
                     VVKVAGHSMMQFRGPARVYEREEDAFVAVKAGQIKAGDVVVIRYEGPSGGPGMREMLG
                     VTAAIVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAVRGGPIAAVKTGDIIVFDIP
                     KRQLNLELPAKEIKARLKKVKPPVPRYTHGVMAKYARHVSSASEGAITT"
     gene            complement(1796..2803)
                     /locus_tag="VFH05_02410"
     CDS             complement(1796..2803)
                     /locus_tag="VFH05_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6677096.1"
                     /translation="MRVMWMIMCACCVAWANADLEAKELSPRDIYEQASPAVVMVMGH
                     SDGGKTGSGGTGSIIQADGLVLTNAHVVVEERTGKPYPRLSVFLKPPRVTGDSRMDLA
                     RMLRAKVVAYSTPLDLAILKLEETPAPLPVVAFSESTHGRIGDRVVAIGHPEQGGLWT
                     LTTGVISAEMDNFNGVKGKHVFQTETGLNRGNSGGPLLDSEGRMIAVNTAIARVATDG
                     LPITSISFSLKSSVAAQWLRDQGLTGSITASTRAPSSQANAASDLKVKPTPQPEPKTA
                     QAPSTAAPVAPPQATKPRGSVEPIPARPYNLDVLISDRAKAEADLEGMMSEMRERMKT
                     R"
     gene            complement(2888..4006)
                     /locus_tag="VFH05_02415"
     CDS             complement(2888..4006)
                     /locus_tag="VFH05_02415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6677097.1"
                     /translation="MNVPLLDLKAQYRAIKAEVMAVVEAVCDEQGFVLGPRVAEFEAA
                     IARYVGCRHGIGCASGSDALLLALMALGVKSGDEVITVPFTFFATAGAISRLGAKPVF
                     VDIDSDSFNIDPHQIERAITPRTKAVIPVHLFGQCADMAAINRIAKPRGIRVIEDACQ
                     AIGAAQDGRRAGLLSDVGCFSFFPTKNLGGFGDGGLMTTDDEGLAGTLSMLRVHGSRV
                     RYLHEAVGINSRLDALQAAVLHVKLRHLDQWTEGRRRNADRYQHLFAQAQLLDRVLLP
                     VTAHGNFHVYNQYTVRVRQRDELRNFLKEKGIGSEVYYPLPMHLQNCYHDLGHHKGSF
                     PVSERAAEEVLSLPIYAELTEEQQTYVVQMISEFYRRG"
     gene            complement(4022..5212)
                     /locus_tag="VFH05_02420"
     CDS             complement(4022..5212)
                     /locus_tag="VFH05_02420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6677098.1"
                     /translation="MDRICHIITKLELGGAQEVALYAVSHLDRTRFRPVLMAGPGGLL
                     TEEARRLPGVDLIIVPTLGRRIHAISDLVAFLHLIRLLRRVKPAIVHTHSSKAGILGR
                     WAAWCAGVPLILHTVHGFGITPAQPNWLQQLLIMVERITGWITTHWVVVAAIDEGRGR
                     RWGLFNANVSVIRPGIDPRPFQQLPDPAARQRLRKEFGAGPDERLVGMVACLKPQKAP
                     EDLVAVAELVCSRMPNAKFVLIGDGALRPRIQALIDERGLHERVRLAGWRRDVDRAMG
                     SFDALLLTSHWEGLPRVMLEAASAGLPIVATKVGGVEEAVVHRDRTRLYEPGDIAGLA
                     EGVEILLQQCRTEGTPPATIRHHLPKEFHIEEMVKQYQSLYDRLLSQRQAKLGSRSLS
                     PASS"
     gene            complement(5219..6547)
                     /locus_tag="VFH05_02425"
     CDS             complement(5219..6547)
                     /locus_tag="VFH05_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6677099.1"
                     /translation="MILIVGAGLAGLSTAYHLSETSYRLYEREMEVGGLCRSYRKDGF
                     TFDYTGHLLHFRQPQIKALVERLLAGKLQKHTRKSFIYSHRTYTEYPFQVNTHGLPPE
                     VVRDCLMGFIATLTTPAAAVPSNDRSFKQWIVDNLGEGMARHFMVPFNEKLWQVPLDE
                     LTSDWVSWLVPKPELKDVISGALGIKDKAFGYNPSFQYPATDGIRVLPESFLPGITGL
                     HQGWELMEVETKRRRAVFKHRASGDTRTEHYESLVSTIPVPELMRRCPDMPTHLKEGA
                     EGLRWVSVYNLNMAVGREQVSDKHWLYFPEPEYPFYRIGFPMNFSPALGRSGCSSMYV
                     EMSHRPSEQHSHEELLSRARSGMERAGILRSEDEIVVADVKDLRYAYVYFDRHRARAL
                     PTMLAELERRGIYSIGRYGAWEHTSMEDAIGQGKQLAERLQARGAEAVSA"
     gene            complement(6544..7587)
                     /locus_tag="VFH05_02430"
     CDS             complement(6544..7587)
                     /locus_tag="VFH05_02430"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HET6677100.1"
                     /translation="MLLLLTFVLACALAIYGVPLARQAALKYGIVDAPDGRLKHQQEP
                     VPYFGGLAIYLAFLMSLAFTFEFRHDVLGIILGGTIVVMLGLIDDFGVLTPATKLAGQ
                     LLAVFVLIKSGIRIEIAAFPEWVDLLLTLIWMIGLINAFNLLDIMDGLSAGIGAVSAI
                     GLFIAAWLQGDQTTAFLLAALIGSLVGFLKYNWKPARIYMGDTGAMFIGLLLGAMAMI
                     GKYPSDHPLSLLTPVFILGIPIFDTLFVMWIRYQRGLPIFWGSPDHIAIRLRHWGMPV
                     PHIVLASYVATAVVAGIGLLLMTVGEDLAWTLCVGTVGVLLLSTYLLKKIDVRRPSSS
                     VASAHAQEGSQAA"
     gene            complement(7735..8865)
                     /locus_tag="VFH05_02435"
     CDS             complement(7735..8865)
                     /locus_tag="VFH05_02435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6677101.1"
                     /translation="MISGERATIHYFSKTTSIGPSSRYRIYQYLPYLQRHGIQTHVYP
                     LFGALYFSMLTWRPAWLRAFGKMVYTVLCFLHRLLHLLVVKAGDLVVIEGQLFPYCAP
                     IVERALSRRNRIILELDDAIYLTPGHARKIPALLRLSAGAIVGNSTLAKYASLHTSNV
                     YVVPTVVDTDRFRPADGKRSSPATAFDRAVTIVWIGLAYNFPYVEMLIPALRELQKHS
                     PIVLRIVSSSPPVLPGLNVEFRPWSYENEVQDLQTADIGVMPLFDDEWARSKCGLKLL
                     QYMAVGLPALASPVGVNRDIVRHGENGFLAASEEDWYRQLAALCRDRELRWRIGDAAR
                     TTVEAQYSLKHWGPALAERYAAIIDDTNRPYSRPAVAQPVVR"
     gene            complement(8862..10676)
                     /locus_tag="VFH05_02440"
     CDS             complement(8862..10676)
                     /locus_tag="VFH05_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6677102.1"
                     /translation="MTPVVELHLRWMIDRAKSVVQKEGVMALLRRMKHRLRSMLRGEA
                     TLTPYAIRDVRDQYRRWRHQHAMTAERLDAIRRDVASLPHSPVLSFAMPLTGGQPQWI
                     ASAIGSFLDQVYPHWELWICAVGDLTEAARQIASDAALKDQRIKLLQPAEGNSIGSMV
                     KVLSSLSNASFVGLMRPHDQLAPEALFEIARCIARYPDTDVIFSDHDVIALDGEYVDP
                     FFKPGWNPDLLLSINYLSPLCLVRRTMLLERSEREGFLGEDDVHAVLLQITDGARRIE
                     HIPQILCHARPDPAAKTAVPGDGLWNRERAEVVAQALQRRGESANVARLERDRYHVSF
                     SRHGMPLVSIIIPTRDRCDLLRRCIDSIERHTPEGRYEIIVMDNGSTEDSSLQFLNSI
                     AGRHRVVQYPQEFNFSAINNEGVAHASGEYLLFLNNDTQVLASNWLPALLAQAQRPGV
                     GAVGAKLLYPDGRIQHAGVVLGVCGVAGHAFRFLRPEDKPYHGLSELVRSCSAVTAAC
                     LLMSKQLFEQIGGFNTRLAVEYNDVDLCLRIRQRGLRIVFDPHAVLIHHENATRKSGR
                     SREDRLVMEELWGDLIEQGDPYYNPNLTSLREDWSLRL"
     gene            complement(10750..11424)
                     /locus_tag="VFH05_02445"
     CDS             complement(10750..11424)
                     /locus_tag="VFH05_02445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HET6677103.1"
                     /translation="MDALRKDSTTVIEEIRRARLTYCGPPKLENLSHAIERIVREGIR
                     GDFIEAGVALGGSAILMGSLKPSNTELHLYDVFGMIPPPGDQDGEDAHRRYDVIKSGA
                     SPGLGSDRYYGYVDSLQDKVLLNMGRWGLSCQKDLIELHPGLFEETLFPSKPIAFAHI
                     DCDWYESVNVCITRILPHLAQGGIMVFDDYHSYSGCKKAVDQLLSSRPELQIIFDARS
                     IGIRFP"
     gene            complement(11429..12553)
                     /locus_tag="VFH05_02450"
     CDS             complement(11429..12553)
                     /locus_tag="VFH05_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HET6677104.1"
                     /translation="MSTDIHAAAHPSQLAVLTPYLIPPALPKRKVLNLGDGFILRAIE
                     RWVGPFSSSRMFSPRLALGDEAVAALTHSSAVIIAGANQLNDRYTIWPGLTAERIRKG
                     RLRFVPFGIGIHGDSEQTIRLSEETRSILEAVHEQIEYSSWRCPNTVAFLRREMPGIA
                     HQLLMTGCPVIYDRPLLESDQFLSAETTVAVTASERGEFWERETQVIDAVTKRFPRAR
                     RYFVVHQNFSPPRMGERLQNLLSSVNAGTCMNRIAALRAYARQRGFKVVIPSSADECI
                     QFYDHVDLHVGSRLHAHLLFLSRNKRSYLVPVDHRSVGIAEHLGFPLLTPEALDRSWD
                     CNFEIVRAKARATYTVMQRFVNSLGRTPCHLNPIANQISV"
     gene            complement(12550..13101)
                     /locus_tag="VFH05_02455"
     CDS             complement(12550..13101)
                     /locus_tag="VFH05_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6677105.1"
                     /translation="MSPKVAAAFADILRKRVYPSRSCSVLEVGASTSTLLSIPLLRDA
                     RKVALNLQFGNPAALSRTAELIIGNGNALGFPDETFDCVMSCSAMEHDAYFWKTAAET
                     RRVLKKGGLFVVGVPIYRTLPTDYRYTTLTYARHGLSYNADFYRFSEQAVREVFFEGY
                     EALTDEVLVRRYPNPYFVVAGRK"
     gene            complement(13134..13727)
                     /locus_tag="VFH05_02460"
     CDS             complement(13134..13727)
                     /locus_tag="VFH05_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6677106.1"
                     /translation="MSIHDSASARMPFNCNDQSNHTWHERAELCAELLASLDDRPGQG
                     FSLADIGCGDQKLREALRRKGLMCRYQGYDMLPGSQDVVRLDVQSECLPCKYDVVVLL
                     GVVEYLEHVEDVFASLALQAPWLLLSHVVQEGEYYTEARRAELGWRTHLTKDQITSML
                     ERSGLAVVRSDMTSDDRTLLMVCRSLRFKEGPRVAGG"
     gene            complement(13724..15025)
                     /locus_tag="VFH05_02465"
     CDS             complement(13724..15025)
                     /locus_tag="VFH05_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HET6677107.1"
                     /translation="MPDICLSVKGISKKFSRSPKRAMVYGAYDLARALLGTTDQTPLR
                     TGEFWALKDVTCTLRRGQSLGIVGPNGSGKTTLMRLIAGVLAPTRGVIQVHGRMAPLL
                     ALGAGFKPVLSGRQNIVLNLSLLGVSSREIHDRMEAIIDFAEIGQAIDAPLGTYSSGM
                     TARLGFACAVHTHPQILIVDEVLSVGDAKFRIKCRNRINQMRRSGTAMLLVSHSAISI
                     QTLCDECLYLKQGAIEAQGTPDKVLAVYESDIVTTLAADNAARLTDVSRPVPGTSPLK
                     ILAVRFTSRNLVDEGAWVAGESGDLVLTMQSVAALENVSVNVIITDLTHRVGEIVQFM
                     TSHRDIGWLHLEPGHPEVRLAFPAVGLRPGTYRVQLSVSQGSMHDIVDAIEDIRLVVR
                     DTGFQPNCLYFQPRDWNLIGGAVSELTVPSRGSELETAEKL"
     gene            complement(15034..15819)
                     /locus_tag="VFH05_02470"
     CDS             complement(15034..15819)
                     /locus_tag="VFH05_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677108.1"
                     /translation="MNTLVASLPLSWRLFRCTLDAKYRRSFLGYVWMVFPAMAITGGV
                     SLASHSGVIRTGETALPYPLFLFVGILIWQVFVDAVDVSHKAFAGARSYLTKVNCPRE
                     AIVLAQLYETLIVAGVRLALLVALVTLFGDGGWRASGILTICFGGALVLGLGVGAHLM
                     PFTILFGDLQQAVKVALTYGVFLTPAFYAPQGTGLFARLLQYSPLAPLMTSARDAAAG
                     MAISQPGFLVAVLGFAVVVLLAGMFMVRLSAPILVERMLVDGP"
     gene            complement(15816..16688)
                     /locus_tag="VFH05_02475"
     CDS             complement(15816..16688)
                     /locus_tag="VFH05_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677109.1"
                     /translation="MEFVTFHAWLRPEESGHPNQSLESAEQVQLIRMMTKSVRLWHPQ
                     SSMTLLTNARTASWRLPSHCKQVINEVMPKDLMLQRAIAQERYVLASDMGAPMVLLDS
                     DILLNGGLDAVFERTFDVALTWRPNHEMPINGGLIILNHERPEVAKGFFSRYVSVYRR
                     KYAEQARWFGDQLALQDCVGLRAEDMSEYQVVERDGCRLLLLPCSSYNFSPHNRYRAI
                     CSRLPDKTVLHFKGQRKRLMTPFWRAWLHPAHSYSPWVQYVGWRERRWIARQAEEERA
                     GLSTALHNHIGRPA"
     gene            complement(16737..>16919)
                     /locus_tag="VFH05_02480"
     CDS             complement(16737..>16919)
                     /locus_tag="VFH05_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677110.1"
                     /translation="TVYFILSFLLAVISFKYFERPIRSLRVHFHENARLRVGLFAGVG
                     FMILVNVAYLVIKSKA"
BASE COUNT         3780 a         5017 c         4730 g         3392 t
ORIGIN      
        1 gccgcgacgg gaaccgtcat tgacaccgaa gtgaaaaagg gcgagaagaa aaaggacaag
       61 aagggaaaat aggttcatgc accacgcgcc acgattgaac agtcgtgatc ttctcgtcgg
      121 ccctggccgg gcaccagccc gcgccatgct gaaagccgtc ggtttcaccg acgaggacct
      181 ttcacgtcct ttgatcggcg tcgccaacac ctggatcgaa gtcatgccct gcaactttca
      241 cctccgtcgc ctgtcggacc gggtgaaagc ggggatccgt gctgccggcg gcacgccgat
      301 cgagtacaac acgatcgccg tctcggacgg tatttcgatg gggacggaag gcatgaaggc
      361 gtcactgatc agccgtgaag tgatcgccga ctcgatcgag ctggtggcac gaggtcatct
      421 gtttgacggc gttgtcgcgc tctcgggctg tgacaaaacc attccgggaa cggtgatggc
      481 gctcgcacgc ctcaatgtgc cgtcgctgat gctgtacggc ggctcgatca tgcccgggca
      541 gtttcaaggc catgacgtga cgatccagga cgtgtttgaa gccgtgggga aacatgcatc
      601 aggcaagatg acgaacgccg agttgaaaga tctcgaagac cacgcctgcc cgggcccagg
      661 cgcctgcggc ggccagttca ccgccaacac catggcgatc gcgttcgaat tcctcggcat
      721 gtccccgatg ggacgcaacg gcgtcccggc catggaccaa cggaaggacg acgtggcctt
      781 cgagtgcggc aagatggtga tggagctctt gaaacgggac ctgcggcctc gccagatcat
      841 cacgagaaaa tcattggaga atgccattgc agccgtggcc acgaccggtg ggtcgacgaa
      901 tgccgtgctg catcttctgg ccatcgccta tgaagccggc atcaaactga acatcgacga
      961 tttcgacagg atcaatcgca aggtcccgct cctggccgat ttgaagccgg gcggaaaatt
     1021 tgccgcagca gacctctacg cggccggcgg gacaacgctg gtggcgaagc gcttggtcga
     1081 cgccggcctg ctccatgcga accaaccgac ggtgagcgga cggaccatcg gagaagaggc
     1141 caaggacgca actgagacgg cggggcaacg agtattgagg ccgctctcag acccgattaa
     1201 ggccacgggc ggattggtga ttctcaaagg gaatcttgcg ccagaaggct gcgtcgtcaa
     1261 agtcgccggc cattcgatga tgcagttccg cggaccggct agagtgtacg agcgtgaaga
     1321 agatgcgttc gtcgccgtga aagccggtca gattaaggca ggcgatgtcg tggtcatccg
     1381 ttatgaagga ccgtcaggcg ggccgggcat gcgggaaatg ttgggcgtca ccgccgcgat
     1441 cgttggagcc ggcttggggg attccgtcgc acttttgacc gacgggcgat tttcaggtgc
     1501 gacacacggt ctgatggccg gccatgtcgc tcccgaagcg gtcagagggg ggccaattgc
     1561 cgccgtcaag accggcgaca taattgtctt cgatattccc aagcgtcagc tgaacctcga
     1621 actcccggcc aaagagatca aggctcgtct caagaaagtg aagccgccgg ttccacgtta
     1681 cacccacggg gtcatggcca agtatgcccg gcatgtctcg tcagcctcgg agggagccat
     1741 cactacatga ctcgcgggcc acaaatggca cgcgtgtgat gcctcgttga cgctcctatc
     1801 tggtcttcat tcgctcacgc atttccgaca tcatcccttc cagatccgct tccgccttgg
     1861 ctcgatcgct gatgagcaca tctaggttat atggacgtgc aggtatcggc tcgaccgacc
     1921 ctcttggctt cgtggcctgg ggtggagcga caggcgccgc agtgctgggg gcctgagcgg
     1981 tttttggctc tggctgcgga gtcggcttca ctttcagatc cgacgccgca ttggcttggg
     2041 aggaaggagc tcgtgtactg gctgttattg agccggtcaa gccttggtcc cggagccatt
     2101 gggccgccac actggacttg agagaaaaac tgatgctcgt gatgggaagg ccgtctgtgg
     2161 ccacacgagc aattgccgtg ttcaccgcaa tcatgcgccc ttcactatcc aacaaaggcc
     2221 cgcctgaatt tccgcgattt aacccggtct cggtctgaaa gacgtgtttg cctttgactc
     2281 cgttgaaatt gtccatctct gcgctgatca cgccggtcgt taaggtccac aggccaccct
     2341 gctccgggtg cccgatagcg acgactcgat caccgattcg gccatgcgtg gactcgctga
     2401 acgcgacaac cggcaacggc gcaggagtct cctcgagctt cagtatcgcc agatccaagg
     2461 gggtcgagta ggccacgacc ttggcgcgta gcatccgcgc gagatccatt ctggagtctc
     2521 ccgtgacacg cggcggcttg aggaagaccg ataaccgggg atagggcttc cctgttcgtt
     2581 cctcgacgac gacgtgcgca ttcgtgagaa ccaacccgtc ggcctggatg atcgaaccgg
     2641 ttcccccgct accggtctta ccgccgtcgg aatggcccat gaccatgacc accgcaggcg
     2701 acgcttgctc gtaaatgtcc cgcggtgaca gctctttcgc ctcgagatcg gcattcgccc
     2761 acgcgacaca gcacgcgcac atgatcatcc acatgactcg catgcacacc tccgtccgac
     2821 gtatggttgg ggcgatgact tgcgctcgtc tgctagctat ggcgcgggtc gacacagcct
     2881 gtgcgcgcta gcctcgtcga tagaattcac tgatcatctg cacgacgtag gtttgctgct
     2941 cttcggtcaa ctcagcataa atggggagcg acagcacttc ttcggccgcg cgttcagaaa
     3001 cgggaaacga tcctttgtgg tggccgagat cgtggtagca attctgcagg tgcatgggaa
     3061 gaggataata cacttcggac ccgataccct tctccttcaa gaagttcctg agctcatcac
     3121 gttgtcggac tctcactgtg tattggttgt acacgtggaa gtttccgtga gcggtgaccg
     3181 gcagcagcac gcgatcaagc agctgagcct gtgcgaagag atgctggtag cggtccgcat
     3241 tccggcggcg gccttcggtc cactgatcaa ggtgccgcag tttgacatgg agcaccgcag
     3301 cctgcagagc atccaaccgg ctgttgatgc caactgcttc atgtaaatac cgcactctgc
     3361 tgccatgcac gcgaagcatc gagagcgttc cggccagtcc ctcatcgtcc gtcgtcatca
     3421 atccgccgtc gccgaagccg cccaaatttt tggtcgggaa gaagctgaaa cagcccacat
     3481 cggacagcag gccggcacga cggccatcct gcgctgctcc gatggcctga caggcgtctt
     3541 caatgacacg aataccccgt ggcttggcga ttctgttgat cgcggccata tcggcgcatt
     3601 gtccgaacag atgcaccgga atcaccgctt tggtacgtgg ggtgattgcg cgttcgattt
     3661 gatggggatc gatattaaat gaatccgagt caatgtcgac gaacaccggc ttggcgccga
     3721 gtctcgaaat ggctcccgcg gtcgcaaaga aggtaaacgg cacggtgatc acttcatcgc
     3781 cggacttgac gcccagcgcc atcaacgcca acaacaatgc atcgcttccc gatgcacagc
     3841 cgattccatg gcggcacccc acgtatcgcg caatggctgc ttcaaattcg gccaccctcg
     3901 ggcccaggac gaagccctgc tcgtcgcaca ccgcttccac caccgccatc acctccgctt
     3961 tgatggcgcg gtactgagct ttcagatcca gcaatgggac gttcacggtg gctccaacga
     4021 actatgatga cgccggactc aacgaacgag acccgagctt tgcttgtcgc tgcgacagca
     4081 gcctatcata cagcgattga tattgcttca ccatttcttc gatgtgaaat tccttcggga
     4141 ggtggtgtct tatcgtagcg ggcggcgtgc cttctgtgcg gcactgttgc agtaagattt
     4201 ctacgccctc ggccagaccg gcgatgtcgc ccggctcata cagccgggtc cggtcgcgat
     4261 gcaccaccgc ttcctcaacc ccgccgacct tggtggccac gatcggtagc cctgccgatg
     4321 ccgcctcaag catgaccctc ggaaggcctt cccagtgcga cgtcagcagc agcgcgtcga
     4381 agctccccat cgcccgatca acatcgcgtc tccagcccgc caagcgcaca cgttcatgca
     4441 agccccgctc atcaatcagc gcttgtatgc ggggccgcaa cgcgccgtct ccgatcaaga
     4501 cgaatttcgc gttcggcatg cgagagcaga ccagctctgc gacggcaacc aggtcttcag
     4561 gagccttttg aggttttaga caggcgacca tgccgaccag cctctcatcc ggaccagcac
     4621 cgaactcttt gcgtaaccgc tgtcgggccg ccgggtcagg cagctgttga aacggtcgtg
     4681 ggtcgatgcc aggcctgatg accgatacat ttgcattgaa caggccccac cgccgtcctc
     4741 gcccttcgtc gattgccgcg acgacgaccc aatgggtggt aatccagccg gtgatacgtt
     4801 caaccatgat aaggagctgc tgcagccagt tgggttgagc cggcgtgatg ccgaaaccgt
     4861 ggaccgtatg gaggatgaga gggactcccg cacaccaagc agcccaccgg ccgaggatac
     4921 cggccttgga actatgagta tgcacgatgg ccggcttcac gcggcgcaac aaccggatca
     4981 ggtgcaggaa cgccaccagg tcgctgatcg cgtggatacg acggccgaga gtgggcacaa
     5041 tgatgaggtc cacgccaggc agtcggcgcg cttcctccgt gaggagtccg ccggggccgg
     5101 ccatcaacac aggacgaaat ctggtgcgat cgagatggga gaccgcatac agcgcaactt
     5161 cctgcgcgcc acctaattcg agcttcgtaa taatgtggca gatcctgtcc acgatcgttc
     5221 aggctgaaac agcctcggct cctctggctt ggagtcgttc ggccaactgt ttgccctgcc
     5281 cgattgcgtc ctccatcgac gtatgctccc acgctccata ccggccgatc gagtagatcc
     5341 cccgccgctc gagctccgcc agcatcgtcg gcagcgcacg tgcccggtgc cggtcgaagt
     5401 agacgtatgc gtagcgcagg tctttgacat cggccacgac gatctcatcc tcgctccgca
     5461 agatgccggc tcgctccatc ccgcttcgcg cacgcgacaa gagctcctcg tgcgagtgct
     5521 gctccgaggg gcggtgcgac atttccacgt acatcgaact gcaaccggac cgtcccaggg
     5581 cgggtgagaa gttcatagga aacccgatgc ggtaaaacgg atactccggc tccgggaagt
     5641 acagccaatg cttatccgag acctgttcac gcccgacggc catgttgagg ttgtacacgg
     5701 acacccatcg gagcccctcc gcaccttctt tcaaatgcgt aggcatgtct gggcagcgac
     5761 gcatgagttc gggaacggga atcgtggaga cgagggattc atagtgctcg gtccgggtgt
     5821 cgccgctcgc tcgatgcttg aagaccgcgc ggcgccgctt cgtctccacc tccatcaatt
     5881 cccacccctg atgcagacca gtgatgccgg gcagaaagga ttcagggagc acgcgaatgc
     5941 cgtccgtcgc cgggtattga aatgacggat tatatccgaa ggccttgtct ttgattccca
     6001 atgccccgct gatcacatct ttgagttccg gtttcggcac gagccaggag acccagtcgg
     6061 aagtgagctc atccaagggc acctgccaaa gtttttcgtt gaacggcacc ataaaatgcc
     6121 tggccatgcc ttcgccgaga ttgtctacaa tccattgttt gaaggagcgg tcattggaag
     6181 gcacggccgc ggccggcgtc gtcaacgtgg caataaatcc catcaaacag tcccgcacga
     6241 cttcgggtgg aaggccgtgg gtgttcacct ggaacggata ctccgtatag gtccgatgcg
     6301 aatagataaa tgattttcgg gtgtgtttct ggagtttgcc cgctaacaaa cgctcgacca
     6361 gcgccttgat ctgcggctga cggaagtgca ggagatggcc cgtataatca aaagtgaagc
     6421 cgtccttgcg gtaggaacga cacagccctc ccacttccat ctcccgctcg tagagccggt
     6481 acgacgtttc agagaggtga taggcggtgc tcagaccggc caggccggcg cccacgatca
     6541 gaatcatgcg gcttgcgagc cttcctgcgc atgggccgat gccaccgagg acgaaggcct
     6601 cctgacgtcg atttttttca gcaaataggt cgagagcagc agcaccccca ccgtcccgac
     6661 gcagagggtc caggcgaggt cttctccgac cgtcatcaag agcagaccga tgccggccac
     6721 gacagcggtt gccacatagc tcgcgagcac gatgtgcgga accggcatgc cccaatgacg
     6781 caagcgaatg gcaatgtgat ccggactgcc ccaaaaaatc ggcaacccac gctgataacg
     6841 aatccacatc acaaacagcg tgtcgaaaat cggaatcccg agaatgaata ccggggtgag
     6901 aagcgaaagc gggtgatcac tcgggtattt tccaatcatg gccatggcgc cgaggagcag
     6961 accgatgaac atcgcgcccg tatcccccat atagatccgc gccggtttcc agttgtattt
     7021 caagaatccc acaagactgc cgatcagcgc tgcgagcagg aaagcagtcg tttgatctcc
     7081 ctgtagccac gcagcgataa acagtccgat cgcactgacg gcgcctatcc cggccgagag
     7141 gccgtccatg atgtccagca ggttgaaggc attgatcagt ccgatcatcc agatcagggt
     7201 gagcaagaga tccacccatt cgggaaatgc ggcgatttca attctgatgc cgctcttgat
     7261 caaaacgaac acggcgagaa gttgaccggc caacttagtc gcgggcgtca gcaccccgaa
     7321 atcgtcgatg agtcccaaca tcaccacgat cgtcccgccc aggatgatgc ccagcacgtc
     7381 atggcgaaac tcaaaagtga acgccagact catgaggaag gccagataga tcgccaaccc
     7441 gccaaagtac ggtaccggtt cctgctgatg tttgagccgc ccgtccggtg cgtccacgat
     7501 gccgtatttg agggcggctt gcctggctaa agggacgcca taaatagcca gcgcgcaggc
     7561 caggacgaag gtcaataata agagcatgtc ttcactacta cttcttaatc aaaagccctc
     7621 ttacgctcct agagcttgct catttcagat taaccgagag ggggcaggat tcctataatt
     7681 ttcagtattt atgatcgagc ggcggggcct actgcgaggt ctgagagaga ccggctaacg
     7741 aacaaccggc tgcgcaaccg ccggtcggga ataaggcctg ttggtgtcgt cgatgatcgc
     7801 tgcatatcgc tcggccaacg ccggccccca gtgtttcagg gagtactggg cttcgacggt
     7861 tgttctcgcg gcgtccccga tgcgccagcg aagctcccgg tcgcgacaga gggcggctaa
     7921 ctggcggtac cagtcctctt cgctcgccgc caggaatcca ttttctccgt gccggacgat
     7981 atcgcgattc acgccgacag gagaggccaa tgccggtaag ccgaccgcca tgtattgcag
     8041 caacttcagc ccacatttgc tccgtgccca ctcgtcatcg aagaggggca tcacaccgat
     8101 gtcggcagtc tgaagatcct ggacttcgtt ctcatagctc cagggccgaa actccacgtt
     8161 gagtccgggg agaacaggtg gagaagagct gacgatgcgg aggacgattg gagagtgttt
     8221 ttgcagttcc cgaagcgcgg gtatgagcat ctcgacgtac gggaaattgt aggccagacc
     8281 aatccatacg atggtcacgg ctctgtcgaa agccgttgca ggagacgagc gcttgccgtc
     8341 agctggcctg aaccggtctg tgtccacgac ggtcggcaca acatagacgt tcgaggtgtg
     8401 caggcttgca tacttggcca gtgtggagtt tccaacgatg gctcccgccg acaagcgtaa
     8461 cagagcggga atctttcggg cgtgtcccgg cgtcagataa atggcatcgt ccagttcgag
     8521 aatgatccgg ttgcggcggc tcagagctct ttcaacgatc ggggcacagt acgggaagag
     8581 ctgcccttca atgacgacaa gatcgccggc tttgacaacc agtagatgaa ggaggcggtg
     8641 caggaagcaa agcaccgtat agaccatttt gccgaacgcc cgcagccatg cgggcctcca
     8701 ggtaagcatg ctgaaataca acgcaccgaa caagggatag acatgcgtct gtatcccgtg
     8761 cctttggaga tacggcagat actggtaaat ccggtagcgg ctactcggac cgatcgaggt
     8821 ggtttttgaa aaatagtgaa tggtcgcgcg ttcgccactt atcacagccg caagctccaa
     8881 tcttcgcgaa gcgaggtcag attcggatta taataaggat ccccctgctc gatcagatcg
     8941 ccccacaact cttccatgac caggcgatct tcccgtgacc ggccgctttt tcgtgtggcg
     9001 ttctcgtggt gtatcagcac ggcgtgagga tcgaacacga tccgcaaacc tcgctgtcgg
     9061 atgcgtagac atagatcgac gtcgttgtat tcaaccgcca gacgcgtatt aaatcctccg
     9121 atctgctcaa ataattgctt cgacatgagc aggcaagcgg cggttacagc gctacagctc
     9181 cggaccaatt cggataagcc gtggtacggc ttgtcttcag gccgcaagaa tctgaaggca
     9241 tggcccgcga ctccgcagac tcccaatacg acgcctgcgt gttggatccg tccatccggg
     9301 tagagaagtt tggcgcccac ggcccctacg ccaggacgtt gcgcctgagc cagcaaggca
     9361 ggcagccagt tcgacgcaag cacctgcgta tcattgttca gaaacagcag atactcgccg
     9421 gacgcgtgag cgactccttc attgttgatc gccgaaaaat tgaactcttg cggatactga
     9481 accacgcgat gccggccggc gatcgaattc aagaactgta acgaactgtc ttctgtgctc
     9541 ccattatcca tgacaatgat ttcgtatcgg ccctccggcg tatgtctttc aatgctgtcg
     9601 atacagcgac gaagcaagtc gcagcgatcg cgggtgggga tgatgatcga gaccaacggc
     9661 atgccatgcc tcgagaagga cacgtgatac cggtcgcgtt cgagtcgggc gacattcgca
     9721 gattcccccc ggcgttgcag cgcttgtgcg acaacctcag cgcgttcccg gttccacagg
     9781 ccatcgccgg gcactgccgt tttggcggcg ggatctggac gtgcgtgaca taatatctgc
     9841 ggaatgtgtt cgatcctcct tgcaccgtca gtgatttgaa ggagaaccgc atgcacgtca
     9901 tcttctccaa ggaacccttc cctctctgaa cgttccagca gcatcgtgcg gcgaacgagg
     9961 cagagtgggg aaagataatt gatggaaagc agcaggtcag gattccaacc gggcttgaaa
    10021 aacggatcga catactcacc gtccaatgcg atcacatcat ggtcggaaaa gatgacgtct
    10081 gtatccgggt accgggcgat gcaccgggca atttcgaaca gcgcctcagg agcaagctga
    10141 tcatgcggtc gcatgaggcc aacaaacgac gcattcgaca gtgaggacaa gaccttcacc
    10201 atggaaccga tcgaattccc ttcagcaggc tgaagcaatt tgattctttg atctttcaga
    10261 gccgcatccg aggcaatctg tcgtgcggct tcagtcaagt ctcccaccgc gcatatccat
    10321 aactcccaat gcggatatac ctggtcaaga aatgaaccaa tcgcggaggc gatccattga
    10381 ggctgaccgc cggtcaacgg catggcgaaa gataggaccg gagagtgggg aagagaagcc
    10441 acgtctctcc tgatggcgtc gagcctttcg gccgtcatcg catgctgatg ccgccaacgg
    10501 cggtattggt cgcgcacatc gcggatggca tatggggtca gggtcgcttc tcctcggagc
    10561 atgcttctca gcctatgctt catgcgacgc aacaatgcca tcactccttc tttttgaacg
    10621 acggacttcg cgcggtcgat catccaccgc agatgaagct caacgaccgg tgtcaaatca
    10681 gcgttcctcc atgtctatcg tccctcgcac ttctcatagc acgaacctgc aatttggctg
    10741 acggttcctt cacggaaatc ggattccaat cgaccgagca tcaaagatga tttgcaactc
    10801 cggacgagag ctgagcaatt gatcgacggc ttttttgcag cccgaatatg aatggtagtc
    10861 gtcaaatacc atgatccctc cctgtgcaag atgaggaagg attctggtga tgcacacgtt
    10921 aaccgattcg taccagtcgc aatcgatgtg cgcaaaagca atgggtttgg acgggaaaag
    10981 cgtttcttcg aacaggccag ggtgaagctc gatgaggtcc ttctgacacg atagacccca
    11041 ccgtcccata ttcaaaagca ccttgtcctg aagcgaatca acatagccat agtagcgatc
    11101 ggatccaaga cctggcgatg ccccggattt gatgacatca taacgacgat gggcgtcttc
    11161 accatcttga tcccccggcg gaggaatcat cccgaacacg tcgtagagat ggagctccgt
    11221 gttcgacggc ttgagcgatc ccatcaagat ggccgagccg ccaagggcca ctccggcctc
    11281 aataaagtcc cctctgatcc cttcccgaac gattcgttct attgcatggc tgagattctc
    11341 caatttggga ggaccacagt atgtcaaacg agcgcgtcgt atctcttcga tgactgtggt
    11401 tgaatctttt ctcaatgcat ccattggttc acactgatat ctgattcgcg atcgggttca
    11461 gatgacatgg tgtgcgacca agactgttca caaatcgctg catcaccgtg taggttgccc
    11521 tggctttcgc tcgcacgatc tcaaaattac agtcccagct ccggtccagt gcctccggcg
    11581 ttaacagtgg aaagccgaga tgttccgcta ttcccaccga tcggtgatcc acaggaacga
    11641 gatagctacg tttgtttcta ctcaggaaca acagatgcgc atgcaggcga ctcccgacat
    11701 gaagatcgac atgatcgtag aattggatgc actcgtctgc cgacgatggg atcactacct
    11761 tgaaccctcg ttgccgtgcg tatgctcgta gcgctgcgat ccgattcatg cacgtgcctg
    11821 cgttcaccga cgaaagcaag ttctgaagcc gttcacccat tcttggaggg gaaaaatttt
    11881 gatgtacgac gaagtaccga cgagcccttg gaaaccgttt tgtaaccgca tcgatcacct
    11941 gggtctcacg ttcccaaaac tctccccgct ccgatgccgt gaccgccacc gtagtctctg
    12001 ccgacaagaa ctgatcggat tcgagcagcg gacggtcata gatcacaggg cagcccgtca
    12061 taagcaattg gtgggcgatc ccgggcatct cgcgacgcag gaatgcgacg gtgttcggac
    12121 agcgccatga ggaatactct atctgctcat gcaccgcctc tagaattgat cgcgtttcct
    12181 cgcttaaccg tattgtctgt tccgaatctc catggatgcc aatgccaaac ggcacaaatc
    12241 gcaacctgcc ctttcgaatc cgttcggccg tcagacccgg ccatatggtg tagcggtcgt
    12301 tgagttgatt agctcccgcg ataataacgg cggagctgtg cgtcagcgcc gcgacagctt
    12361 catcgccaag agcgagccga ggcgaaaaca tcctggatga ggagaacggt cccacccatc
    12421 gctcaatcgc tcgaagaatg aatccgtctc caaggttcag gaccttcctc ttcggtaagg
    12481 cgggaggtat gagatagggc gtcagcaccg caagctggct cggatgcgct gccgcgtgga
    12541 tgtccgtgct cattttcgac ctgcaaccac aaagtaagga tttgggtaac ggcgcacgag
    12601 cacctcatcc gtgagggctt catacccttc aaagaacact tcgcggactg cctgttcgct
    12661 gaaacgataa aaatcggcgt tgtacgacag gccgtgcctt gcataggtca atgtcgtata
    12721 tcgataatcg gttggtaatg tgcgataaat cggaacaccg acgacaaaca gccccccttt
    12781 cttcagaacc cgcctcgttt cggctgccgt cttccagaag tacgcgtcat gctccatcgc
    12841 ggaacagctc atgacgcaat cgaacgtctc gtccggaaat cccaaggcat tgccgttgcc
    12901 aatgatcaac tcagccgttc tcgataaggc cgccggattt ccaaattgga gattgagcgc
    12961 gactttcctg gcatccctga gaagcggaat cgacaataga gtgctggtac tagcaccgac
    13021 ttccaagacc gagcaagagc gagaggggta aacccgtttt ctcaagatgt cggcaaacgc
    13081 cgcagcgact tttggactca tggcctttcc agcatccgtc cgacggcgac ctctcagccg
    13141 ccggccaccc gaggcccttc cttgaatcgc agggagcggc ataccatgag cagcgtccgg
    13201 tcatcagagg tcatgtcgct tcggacaaca gccagcccgc ttctctccag catgcttgtg
    13261 atctggtctt ttgtgagatg agtgcgccat ccgagttcag ccctgcgcgc ttctgtgtaa
    13321 tattctcctt cctggaccac atggctcagg agtaaccatg gcgcctgaag cgccagcgaa
    13381 gcgaacacgt cttccacatg ttccagatat tcgacgaccc caagcagtac cacgacgtca
    13441 tacttacaag gcagacactc tgactgcacg tccaaacgga cgacgtcctg gctccccggc
    13501 aacatgtcat atccctggta ccgacacatg aggccttttc gccgaagagc ttctcgaagt
    13561 ttttgatcgc cgcagccgat atcggcaagc gaaaacccct gacccggacg gtcatcaagc
    13621 gaggcaagca gttcagcgca caactcagca cgctcgtgcc acgtgtgatt gctctgatca
    13681 ttgcagttga acggcatccg cgccgatgct gaatcgtgaa tgctcacagc ttctccgcgg
    13741 tctccaactc agaccctcgt gaagggactg tcagctccga tacggcgccg ccgatcaagt
    13801 tccaatcacg cggctggaaa tacaggcagt ttggctggaa gcccgtatcg cgtacgacga
    13861 gtcggatatc ttcaattgca tcgactatat cgtgcatcga accttgggaa acactgagct
    13921 gtacccgata tgtccccggt cggaggccaa cagcggggaa agccagacgg acctctgggt
    13981 gccccggctc aagatgcagc cagccgatat cgcggtggga ggtcatgaac tggactattt
    14041 cgcccacccg atgggtgaga tcggtgatga tgacattgac gctgacgttc tccaaggcag
    14101 cgacactctg catcgtcagc acaagatctc ctgattcccc cgcaacccag gctccttcat
    14161 caacgagatt ccgcgaggtg aagcggactg ccagaatctt gaggggtgac gtacccggta
    14221 ccggccggga cacgtccgtg agccgggcgg cattgtcggc agcgagcgtc gtcacgatat
    14281 ccgactcgta caccgccagc accttgtcag gagtaccctg cgcctcgatc gccccctgct
    14341 tcaggtacag acattcatca cacaacgtct ggatcgatat cgcactgtgc gaaacgagca
    14401 gcatcgccgt cccgctcctt cgcatctgat taatacggtt tcggcatttg attctaaact
    14461 tcgcatcgcc gaccgacagg acttcgtcca caatgagtat ttgcggatgt gtatgcactg
    14521 cacaggcaaa acccagtctc gccgtcattc ccgaactgta cgtcccgagc ggtgcatcga
    14581 tggcctgtcc gatctccgca aagtcgatga tggcctccat gcgatcgtgg atctccctgg
    14641 aagaaactcc cagaagagac agattcaata cgatgttctg gcgaccggac aggaccggct
    14701 tgaatcccgc accgagcgcg agcagcggcg ccattcgtcc gtgaacctga atgactccac
    14761 gcgtcggtgc cagtacccct gcgatcaaac gcatgagcgt cgttttcccg ctgccgtttg
    14821 gtcccacgat gcccaacgat tgccctcgtc tcaacgtgca ggtcacgtct ttgagagccc
    14881 agaattcacc ggttctgagc ggcgtctgat cggttgttcc cagcagcgca cgggcaagat
    14941 cataggcgcc atagaccatc gcccgtttgg gcgagcggga gaatttcttg gaaattccct
    15001 tcacggacag acagatgtca ggcatgacgt ccattagggc ccgtccacca acatgcgctc
    15061 gaccaggatc ggagccgaga ggcgaaccat gaacatgccg gcaaggagaa caacgaccgc
    15121 aaatccgaga accgccacga ggaagccggg ttgagagatt gccattccag ccgccgcgtc
    15181 gcgtgccgat gtcatgagcg gggccagcgg cgagtactga agaaggcgtg caaagaggcc
    15241 ggtgccttgc ggcgcataaa aggccggtgt caggaacacg ccataggtga gagcaacttt
    15301 taccgcctgt tgcagatccc cgaagagaat cgtaaagggc atcagatgtg ccccgactcc
    15361 cagacccagc acaagagctc ccccgaaaca gatcgtgagg attcccgaag cacgccatcc
    15421 tccgtcacca aacagcgtga ccagggcaac aagcagtgcc agcctgactc ccgcgacgat
    15481 caacgtctca tacaattggg cgagaacgat cgcctcacgg ggacaattca cttttgtcag
    15541 gtaggagcga gcgccggcga acgccttgtg agagacatcc accgcatcca cgaatacctg
    15601 ccagatcagg atcccgacaa acaggaacaa cggatacggc agagcggttt ctcccgtccg
    15661 gatcaccccg gaatgtgacg cgagcgaaac cccgcctgtg atggccatgg ccggaaacac
    15721 catccacaca taaccgagaa atgatctcct gtacttcgca tcgagcgtgc aacgaaagag
    15781 ccgccacgac aacggaagcg aggccacaag cgtattcatg ccggcctccc aatgtgattg
    15841 tgcagtgccg tggacaagcc ggcgcgttct tcctctgcct gacgggcgat ccaacgccgt
    15901 tctcgccacc ctacatactg gacccagggt gaatacgaat gcgccgggtg cagccaggcg
    15961 cgccagaacg gagtcatcag acgcttccgc tgtcccttga aatgcagcac ggtcttgtcc
    16021 ggcaaccgcg agcaaatggc tcggtaccgg ttgtggggag aaaagttata gctgctgcag
    16081 gggaggagca acagccggca accgtcgcgc tcgacgacct ggtattcgct catgtcttcg
    16141 gcacgcagcc cgacacagtc ctgtagggcc aactggtctc caaaccatct cgcctgctcg
    16201 gcatacttgc gccggtagac cgagacatag cgtgaaaaaa agcctttcgc gacttctgga
    16261 cgctcatgat taaggatgat cagcccgccg ttgatcggca tctcatgatt cgggcgccaa
    16321 gtcagggcca cgtcgaacgt gcgctcaaac actgcgtcaa gacctccgtt aagcagaatg
    16381 tcagaatcca acaacaccat cggcgcaccc atgtcgctgg caagcacata ccgctcctgg
    16441 gcaatggccc tttgaagcat taagtctttc ggcatcactt cgttgatgac ttgcttgcaa
    16501 tgcgagggca gccgccaact ggctgttcga gcgttggtta atagagtcat ggatgactgc
    16561 ggatgccaca gtctgaccga tttggtcatc atgcgaatga gttgaacctg ctcggcggat
    16621 tcgagcgatt gattcggatg ccccgactct tcgggacgga gccatgcgtg aaaggtgaca
    16681 aattccatat acactcttca gagccctcgg cggacgtcgt tcaacgtccc cggacgttag
    16741 gcttttgact tgatcaccag gtaggcgacg tttaccagaa tcatgaagcc cacccctgca
    16801 aataaaccga ctcggagtcg tgcgttttca tgaaaatgca cgcgcaagct ccggatcggc
    16861 cgttcgaaat atttgaatga gatgacagcg agaagaaacg acaggatgaa atagacagt
//
LOCUS       DASSCQ010000038        31157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1339534, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000038 DASSCQ010000000
VERSION     DASSCQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 31157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31157
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339534"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="VFH05_02485"
     CDS             complement(<1..1703)
                     /locus_tag="VFH05_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6677111.1"
                     /translation="MATPSLSINPAFLILQARSVVVSPYSRVAYILAALTLFAPLIEG
                     GTTHFPVSVMRTVLFLSCLTWILSSRKDGSMSLQWNTVSPWILCFLTLAAVSVLWTPY
                     KHAAVQWVVTLAMYAIFFQLAYQGVKSFRDVRILVIIILATGALQAALGILQYGIFDE
                     YRAHGTFFNPNFFASYQAGIVVLTGSLLCFSGDLDISNGKRAALWALLIVSVVAFVLA
                     RSRGALLALVPVLFFLALMRFRRRAIPFLLCGLLAVIIIPNPIRARSLEVASQDHFAY
                     TRLDIWKNAMARIADQPWGSGLGMYKYVSFKSRFPIEGELARYGRRAESAHNEYLQIA
                     SELGLAGLFIVLTGAVLWVKEVKYVCVDQARSATRAIMIGLAGIVSAILIHALVDSVF
                     HEPALVLLLILAAAMVLAMGRMRCSEDPAQPQLVIGYTRCRALIGGLILSILLVLTVQ
                     PAAAWYAFEQGNSALSSGDLRTSVRWYAVAIKIEPWNASYHNALASSEIALFQQSKDP
                     AWIVDAVEEMKMCSTLNALDGRYPYRLGTLYQLLAETAETSQEKSARRLAEATDSFRQ
                     AV"
     gene            complement(1905..2420)
                     /locus_tag="VFH05_02490"
     CDS             complement(1905..2420)
                     /locus_tag="VFH05_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6677112.1"
                     /translation="MCKSLKGQKGFTLIELMIVVAIIGILAAIAIPNFIAYQSKSKQA
                     EAKVSLGAIFTSATAYNAETVPQTYLAAQANLSGIGWAPSGTPRYTFWYAWDAAAITG
                     PAVGPGTPAMFPMPSVPATCNVAPTTGFTVAASPSGFTAGANGNIDGDPNCDQWYIND
                     ERRLNNTQNDV"
     gene            complement(2894..4723)
                     /locus_tag="VFH05_02495"
     CDS             complement(2894..4723)
                     /locus_tag="VFH05_02495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677113.1"
                     /translation="MELYSTSAAWSLRCLCALMPLSFLAAAACASPSKSPEGAVQTAQ
                     PAKPASSARPADATAAYHFMLGYQAELSQNSEQALKEYEAALKADPNALSVKARLASL
                     HFSLGDMPNALRYAEEVADGRAEDGRMLTHMAGILAGGGKGDKALSVLDRAVELDPES
                     GDAYFSKGLLLLNLKRPAEAEQAMRAGIARSPDNAVGHYQLGRVLLEAGKVEEATTSF
                     ERAITANYSFEPAYLALAAIYESRHEKERAVGVLKRYLQQVNSRNRDIRHQLVRLYID
                     AKDYSGARKELEAMIAEDPSDLDAQLRMALIHGEQKEYPQAIERLTEILKVRPAELKV
                     RDYLGYVYEESKDTQKALETYTFNVQLEPTFFEGHLHLGVLYYRLKKFPEATEHLGRA
                     IALNPKQPESHIVQGLAYLQQDQYDKSAEVFQEGIRHNPKSADLHFNLGTAYDKLDRF
                     DDVIREMETAIQLDPHHADALNYLGYSYADRGIKIDQAISLTKQAVALKPENGYYVDS
                     LGWAFFKSGLLTEALAELKRAAALVGDDPVIYEHLGDIYAKQQRLSDAREAWLHALEL
                     DPSNHKLMDRFREQGMGDPAQEERIQQAKRRVAGQKPSLPIAQ"
     gene            complement(4803..6158)
                     /gene="dnaB"
                     /locus_tag="VFH05_02500"
     CDS             complement(4803..6158)
                     /gene="dnaB"
                     /locus_tag="VFH05_02500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET6677114.1"
                     /translation="MKSMPGIDLSQPKLPPQNVEAEQSVLGAILLDNQAMAKAMELVV
                     EENFYRTAHRKIYRAMLELSETGEVIDHITLTERLKAQGELETIGGAAYLAELVQMVP
                     SSANIRYHSKIVRDKALLRELLHTSTEVLTRGYEGTAPIDELLDFAERSVFSIAQGKL
                     DRSFSPINAIIKESLDLVDKLSKRKEQVTGVPTGFYDLDDLTAGLQPSDLVVIAGRPS
                     MGKTSLVLGMAAHAALHAGTSVGIFSLEMSKPQIVLRMLSSEARVDSHALRTGKLQKE
                     DWWRLAEAAGRLEQAPIYIDDTGGITVQQMRGKARRLKAERGLDLLIVDYLQLMQGRS
                     DAESRQQEISDISRSLKALAKELNVPVVALSQLSRAVEARKPPIPMLADLRESGAIEQ
                     DADVVMFIYREDVYDQNSERKGIADILVSKHRNGPIGKKELFFHDRFAKFESLDNREV
                     V"
     gene            complement(6155..7477)
                     /gene="hisZ"
                     /locus_tag="VFH05_02505"
     CDS             complement(6155..7477)
                     /gene="hisZ"
                     /locus_tag="VFH05_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET6677115.1"
                     /translation="MATILPHAAKQVRRIEAQLLSQISRWGYDEIILPTFEYLDVLAP
                     GLEAELLETCYQFIDRTTGRTLLLRPDATAQVARTIGMGLTGSMLPQRLSYRTSVFRY
                     EPEHAGRGREIFQVGAELVGVDDVTGDYEIISLLIECLQAIGLRSFTIAVGHVGFIKG
                     LLMRSGLSAQGQKRAEQAAAKKDLPRLEELLASERIAKAAAKAIREAPELTGHEAVLE
                     RGRVLAAGEPALTAPLDRLAQVYHLLCAAGHRDSILLDLGEFRGFDYYNGVVFDVFAP
                     GVGAELGGGGRYDHLIGRFGRDLPSTGFALDVDHLFRAIEMPQPGVDGPRLDYVVAGL
                     RRSMRAVIEIADTLRRTQRRVVQVLIKGSEHTLLSNALEAAQAQGAHALIVIGAPGMD
                     QDHVAYFEKLGGADGRRNGRPHRPAMKKLTRAALLAGGRSRRRRKESK"
     gene            complement(7615..9198)
                     /gene="serA"
                     /locus_tag="VFH05_02510"
     CDS             complement(7615..9198)
                     /gene="serA"
                     /locus_tag="VFH05_02510"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET6677116.1"
                     /translation="MKILVSDSLSKQGVELLERAGFTVVVKSKMPKDELFREIKDADG
                     LIVRSGTKVTEELIAAAEKLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNTVTTAE
                     HTMSMICAMSRRIPQATTSVKAGKWEKDKFMGVELFNKVLGIVGVGQIGSHLTKLAQG
                     VGMRVIAYDPYLATDRAEKMGVEMLSIAELFRRADIISVHTPLTPETKNLINAQAIET
                     MKPGVMIVNCARGGIIHEGDLYEALKSKRVAAAAFDVFEEEPVKADHPLLSLDNFICT
                     PHIGAQTTEAQENVAVGIAEQIVDYFTKGIARGAVNIPSVSPELLPRLQPYLSLAEQL
                     GRLETQLCEGGLERVTVEYSGDVTTLSIAPLTIAVLKGLLTPIMESPVNYVNAPIVAK
                     ERGIEVKEVKSTDAGDFTSLIRVRVEAGKQSRLVAGTLYHKKEPRVVEIDQFKVEVVP
                     EGHMLFIHNIDRPGVIGMVGKVLGDNNINIVRMQCALEKRGGNALLIIGSDQAFPAHV
                     LNTIQSSKDIVSVKVAALS"
     gene            complement(9208..10350)
                     /locus_tag="VFH05_02515"
     CDS             complement(9208..10350)
                     /locus_tag="VFH05_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET6677117.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDPIFAEAREG
                     LKWLFQTRNDVLMLAASGTGGMEGSVSNFLSPGDKALCINGGKFGERWGKICKTFGVQ
                     AIELKVEWGHAINPQLVKDALKKDPAIKAVYVQASETSTGVSHDVKALGEIVKAHDQV
                     ILVVDAITALGVFDIKTDAWGLDVVITGSQKALMLPPGLAFVSVSDKAWALADKAKNA
                     AFYFNFKKERENQVKNQTAFTPTVSLIIGLQEVIKMLKAEGLDKIFARQGQLAQAMRE
                     GMKAAGLALFPKESPSDALTAVSAPDGVDGQAVYKNLRVQYGITAAGGQDHLKGKIFR
                     LSHMGYADRFDVITAIAATEMVLKGLGHPVKLGSGVAKAQEILMAK"
     gene            10596..11981
                     /locus_tag="VFH05_02520"
     CDS             10596..11981
                     /locus_tag="VFH05_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6677118.1"
                     /translation="MLHIRFHNGVLGRLLKSQAGFLGLLLCLAPSPGLTETLTDSDIA
                     AQLAEAPPVINSTDEPAIDPADEPDANLVPVETELPPDSTASATSKPDSRVPRAEDLL
                     VITPPQQSSTARFTDAFDAPAETAETVPSLSGLTEPSELYNVPIVMDSSVQSHIRYFN
                     TSIRNRFEQWLLRLSRYRPLVENIFAEFHLPSDLVFLSLVESGFNPYAYSRAKATGPW
                     QFMKGTAKVYGLRVDAYVDERRDPIKSTVAAARYLRDLYDLFGAWPLAMAAYNAGEGK
                     VMRALQKAQAESFTDISKTRLIRRETKEYVPRFMAATIIAKNPNRYGFPQEASAPHQF
                     EEVVVTRPLHFHAIANATGVPFSELRLLNPELRRDATPPGDDAYLLKVPVGTKAKVEQ
                     LLDRVPTYKFPREAKPQFASAGSRWYKVRVGDTLEKVSRRFRIPLKTLKTRNNLTGPT
                     IRPGDFLVIGR"
     gene            complement(12031..12798)
                     /locus_tag="VFH05_02525"
     CDS             complement(12031..12798)
                     /locus_tag="VFH05_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677119.1"
                     /translation="MSYRIKVPAKTLQVDEAHLLSGLEHQLHRLQEYRLPLLVGFAVL
                     LLAVAAVGGVLWMDRQASQKAHELEREAARLLTARSTADPKNADTLLTQALAKYREVI
                     EQYPRTSTAPLAMYHLGNTQAQANDLPAAIETYERFMLLYGSHSALAGLVQQRLAYSY
                     LLKGERDQAAKAFAAIVERPGALLKDQALFELARLEESQSRPEGALARYQELIKTYPT
                     SPFASEATIRTKVLDVKPKEASPQSPPAVPASPEKKP"
     gene            12940..14103
                     /gene="bioF"
                     /locus_tag="VFH05_02530"
     CDS             12940..14103
                     /gene="bioF"
                     /locus_tag="VFH05_02530"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET6677120.1"
                     /translation="MMFEDYLAQLASKHLTRRLLPLESGTGPVVRIAGRDVLLFASND
                     YLGLAGHADVVDAAVTATQTYGAGAGAARLVSGSLPPHQHLEEALARFKGTESALTLG
                     SGYLANIGAIPALISSGGLILADRLCHASLIDGCRLSGADVRIYRHNDMEHLARLLSK
                     RRVGRRTLIVTDGVFSMDGDLAPLGDLVALAEAYQADLYVDDAHGTGVMGTHGRGTIE
                     HFGLEKRIALHMGTMGKALGSSGAFIAGTRHAIQYLLHRCRSFMFATASPPASAAAAL
                     AALRIIEREPERRRRLWDNRQRLFEGLRRLGFTMAPSVSPIMPIVIGRADTALRFAEH
                     LLANDVFAPAIRPPSVPADTSRIRVTVTSEHTPAQIDQALAAFERTGQDTRVL"
     gene            14218..15279
                     /locus_tag="VFH05_02535"
     CDS             14218..15279
                     /locus_tag="VFH05_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET6677121.1"
                     /translation="MDISKLLTFSAKEGASDCHISAGEPPMIRLHGDLKKLDHPPLTA
                     EETHALVYDMMSDAQRKNFEEKRECDFSFELGDIARFRVNVFVQNRGLGAVFRNIPTE
                     IIPMEKLGLPPIVRQLCDKEKGLILVTGPTGSGKSTTLAAMVDFLNNTFEGHIITIED
                     PIEFVHKTKKCLVNQRELGVHTLSFANALKSALREDPDIILVGEMRDLETIQLALTAA
                     ETGHLVFGTLHTSSAPKTIDRIIDAFPPAQQAQIRTQLSEALEAVITQTLLKKKAGGR
                     VAALEVMVATTAVRNLIREGKLHQIPGIMQASQKDGMQTMDMALVELATRGVVTKAEA
                     QSRSMSPNLFGGSALSGAA"
     gene            15307..16482
                     /locus_tag="VFH05_02540"
     CDS             15307..16482
                     /locus_tag="VFH05_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET6677122.1"
                     /translation="MDIRTLLKVMVDQEASDMYLTVDSPPSYRVHGSTQPANTPPFTN
                     EQLEALALALMRGQQRSEFEEKMEMNLALYYKELGRFRVNIFRQRGNVGLVFRHIKAE
                     IQTVEQLRLPQIMKDIAMTKRGLVLVVGATGSGKSTTLAAMIDHRNSVHPGHIVTVED
                     PIEFVHNHKKSLITQREVGFDTLSFQNALKNTLRQAPDVILIGEIRDTETMEAAITFA
                     ETGHLCIGTLHSNNANQAIERIMNFFPVERHAQIYLQLSLNLRAIVSQRLVPSTDGKR
                     IPALEIMMDTPRIKDLIKKAEVDTLKEAMEQGIEEGCQTFDYVLLQLYKENKITLEQA
                     LINADSANNLRLKIKLEGLKGDDAINALLDKGPHATGESPFKIQGGGPGNVRPIRKL"
     gene            complement(16509..16991)
                     /locus_tag="VFH05_02545"
     CDS             complement(16509..16991)
                     /locus_tag="VFH05_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677123.1"
                     /translation="MSDRTVNVLLMSVLLIGLVWAYFSARDPAVQTAPPVPLLAREMV
                     PLVTGEEPLAAIFTRTGCPVCHQIPGIPGADGRVGPPLLLGTVGPQRLRDPAYGGKAK
                     TVHEYVVESVLEPGVFVVPGYPPETMPTWYGAKLSALALEKIAAYLEQQGTAEDSRKQ
                     "
     gene            complement(17073..17924)
                     /locus_tag="VFH05_02550"
     CDS             complement(17073..17924)
                     /locus_tag="VFH05_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6677124.1"
                     /translation="MGNWGLITGVIALLAAGALTVAAGPPPKDAPPGIETVKGKDGAP
                     MILIPAGPFTMGSNEGLPNERPEHIVELDAYYIDQYEVTLALYRKFLDAGKHDSPPTW
                     DDEAATTVGDRPAIGMKWADADAYCKWAGKRLPTEAEWEKAARGTDARRYPWGSMQPF
                     VDIANYNRGLWVSEAVTLAAVTSGLEGMSVRHGLKEGGKSPYGLNHMAGNAAEWVSDW
                     YDRDYYQNGPKKNPTGPAQGEKRVLRGGSWADLPLALRVTARISAEPEFEDRTIGFRC
                     AMPVSGQ"
     gene            complement(18223..18654)
                     /locus_tag="VFH05_02555"
     CDS             complement(18223..18654)
                     /locus_tag="VFH05_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677125.1"
                     /translation="MSQVSRRVNRKSTRTDVIRVLLVDDVESVRQTLRSILATYPNFE
                     IVGEACNGEEALESVQLLKPSVVVMDINMPRLDGIKATTLIKRVYPHIVVVGLSVYAN
                     DDTRKAMTAAGATTVISKEAAVEQLRDEIIESVNRRSAAIH"
     gene            19040..19273
                     /locus_tag="VFH05_02560"
     CDS             19040..19273
                     /locus_tag="VFH05_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677126.1"
                     /translation="MSTHRYKGHTIALIPSMEGLMWACQYVIMKADQTEIAGFPDGNT
                     YDDRQQAELAALAKAKSLIDESALNKDPLGSGR"
     gene            20105..20791
                     /locus_tag="VFH05_02565"
     CDS             20105..20791
                     /locus_tag="VFH05_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HET6677127.1"
                     /translation="MWYFRRRMWWVFLLIGCSMPNTLVQAEMYVAGQVGVTLPQSLSN
                     VEVSDPSIGLPSGSTFSNLDLQNSVMYGAKLGYYFESLKWLGVETEVFNTTPHVKQQP
                     ITVTVPGSPSFTINPAGSYLRVLTWAPINLVVRYQMGKFEPYAGVGMGIFFSRLKDAG
                     SNDSTSSTSVGLNTQVGLRYRVTNNVALFGEWKFNHARLGFDATPNAFTNADATYNAH
                     HLVFGVGYHF"
     gene            21625..22188
                     /locus_tag="VFH05_02570"
     CDS             21625..22188
                     /locus_tag="VFH05_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HET6677128.1"
                     /translation="MRHATANARWKEPMRGLLIVVACCALVSACSPSVFPYKALDGVN
                     QDFDFAGWQLLQEPHPQQKVQLGGRIVQAEMKDDMVTITATQLSIPKNPTHEPEEDKS
                     SGEFLIQYSAVIDPLVLRSGNKLIVVGETGSPGQIEVEKKVRLLPKITALCIHFWNTD
                     GNGIVLRGSSSAGTKFLAELTYCKTAL"
     gene            22451..22690
                     /locus_tag="VFH05_02575"
     CDS             22451..22690
                     /locus_tag="VFH05_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677129.1"
                     /translation="MSQRVKTNYSYKGWNITLVMTLEGYMWTCQYIIRRSGRTVMDGF
                     PGSTYLSREEAEETALSTAKGLIDQCQLDKNPLGI"
     gene            complement(23353..23619)
                     /locus_tag="VFH05_02580"
     CDS             complement(23353..23619)
                     /locus_tag="VFH05_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677130.1"
                     /translation="MARRKRVRTGTVEWNGNDWRRAWKIVLTRIHGAPAVVMEQMAFN
                     HAVDMLDCGFARGDAFQFQLGMLMMIDCCNEAILRGDCYQWWIE"
     gene            24043..25830
                     /locus_tag="VFH05_02585"
     CDS             24043..25830
                     /locus_tag="VFH05_02585"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6677131.1"
                     /translation="MYSRFYGFTTEPFQNTPDLDFLFLSTNHKEALACIEYGIRQRKG
                     FIAITGEAGVGKTIILRSYLTKVDAQKQKTIYILNPRVSFDSVLTTILKELSAEPVAG
                     PATQKLNQLHEVLIAEYRNDRTVVLIIDEAQNMPIETLESIRLLLNLETAKEKLIQMV
                     FVGQPELDTLLNRYELRQVRERIALRARIAPLTKTESEDYIDHRLSLVRGEPRAVFSK
                     QALDRIVKSANGIPRRLNILCDAVLIAGFEYRQAIISDRIAKEAIAAFIGLSQPPRRL
                     QPRYAVGMLSVVLMVVFYLVYLSTMPGITVSGPSEDTVQRKEPPKPVLGTIGADQLFA
                     RDRDPDSVPPARLVSEFEPKEGNDLSPEERMELSETARLLAVLLDSGRVVLGRAQPTI
                     NNPRIGDKGFSSSVFEGQLRAEFQTRTRHDLHNLAVAPMPESAKHLLLRLAFFMQKAV
                     HDVQADINKKGIGFKGFIPATFGTQVAYSFSKDTGLKLRQIGPPGTEPRNPDNKPNEQ
                     EEQLLYAVQKNHPRVGDHIVEQQLADIRGVRVMLPLFYSKQCLACHGNPKGQVDISGY
                     PKEGFKEGDLGGAISIVLSAQKKLTKSSE"
     gene            25827..27608
                     /locus_tag="VFH05_02590"
     CDS             25827..27608
                     /locus_tag="VFH05_02590"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET6677132.1"
                     /translation="MSLSYQQSTQWYQSAVDQLTRIAAAMQRQEHFDLKRLSSLASEM
                     VESLNRGDQLVVEALAGSSGSPLITNLINVGILATKVGVGLGYSGMELERLALAGLVH
                     DIGLFAFPQSLVTKAGHLTPEERMLVEQHPEVGYQALGKLGTTHDWLAEVVRQAHERW
                     NGQGYPNKLKGRQIQEFAQIIGAVDVFEALISPRPYRRRFLPHEAVRELVAVERTAFR
                     REVIKALVEQLSAYPIGTSVRLSTGELGTVIHVNTHFPLRPVVLIGEGRTEGLAARQV
                     DLSTSPRVTIIETVEPPAIARISFPIRTLALPVTGHPFPAVLSPRNRAALDAIANVIQ
                     EVVESVAPVRRGVDRLSEGETTWPPRRTAVQDSFIEAPSSHSLSNRQQAATPQAVIQS
                     SEASEPPLLKALRALQQARARSAQPTRDVLETVILRAGHEAGEIGVSVIEHILNELDR
                     LDEQFIEDVRRRVPVIVQALHTLRSQETTGFVTASHIDPIVCEVDALYDLANRVEASM
                     MTTFLHGLRSLLRYDLANRVEASMMTMIRHGLRSLFLARADRKASTLPQWLETVEARV
                     QTLIPMAEQWVNIGRTERADISEILRA"
     gene            complement(27772..29187)
                     /locus_tag="VFH05_02595"
     CDS             complement(27772..29187)
                     /locus_tag="VFH05_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6677133.1"
                     /translation="MYESYYHLRTKPFSLLPDSDFLYAGSTHRAAYSTLEYGIIHEAA
                     FMVLTGEPGMGKTSLLRKLIAEHGTKHSIGLVTNARYNIEHLLPWVLLSLRLSKRQLD
                     PVEAYYVFSEYLAHESQQHRRVILIIDEAQSLGTDLLEELRLLSNLNDGKTLKLQIVL
                     SGQPDLHKLLQRIDMTQFAQRVAVDCHLELLSEVETGQFIHHRLRVAGGHPSLFTNKA
                     CALVHRLTKGNPRLINQVCDLTMTHGFGLQAQVITSKIVAQAALERSKGKILPLSSRE
                     ELMVLSNLPDDTREIDAPTPQPTPAQWLENPHPERPATPAISPEPFFYAKGVAMRKEG
                     RPKEAIEVLELAGKSPAYRLKAQVQIGLCHRAAGEHRTAIKIFRAALDDKSASRTELL
                     DIQYFLARTLETAGQIAEAVEIYRRIADTNRRYKDAASRVHSLSSKVRYLTNGEQPAV
                     NQSWIGNAIKSFPGWPLLVTR"
     gene            29648..29779
                     /locus_tag="VFH05_02600"
     CDS             29648..29779
                     /locus_tag="VFH05_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677134.1"
                     /translation="MKHVISSSIERLVSFALFTLGIAAVAVAAAALLLIIGDTLGHA"
     gene            29797..29955
                     /locus_tag="VFH05_02605"
     CDS             29797..29955
                     /locus_tag="VFH05_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677135.1"
                     /translation="MRHAIFDTLFFALLTAATAAVLFSIGISLGHAQSSWKDGKPTLC
                     IATKLGDV"
     gene            30547..30690
                     /locus_tag="VFH05_02610"
     CDS             30547..30690
                     /locus_tag="VFH05_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677136.1"
                     /translation="MAISSKGRNYWSRGGNPIGILPAASTFYVHGLGNDIKVGWGCFH
                     RGR"
     gene            30783..>31157
                     /locus_tag="VFH05_02615"
     CDS             30783..>31157
                     /locus_tag="VFH05_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET6677137.1"
                     /translation="MKDTKPFAGLIVRPLAAFATLMVLMSSSEAGSLDRTVLPIAEPQ
                     RPTYTELDVRKVTPPPHFEVKAPEGAPNILVVLVDDLGFAGTSAFGGPVGTPTFDRIA
                     HEGLKYNNFLTTAVCSPTRAALK"
BASE COUNT         6812 a         8941 c         8601 g         6803 t
ORIGIN      
        1 actgcttgcc ggaaggagtc ggtcgcctct gccaatcgac gcgcgctctt ctcctgagat
       61 gtctcggcgg tttcagcgag caactgatag agcgtgccca ggcgataggg atatctgccg
      121 tccagggcgt tgagtgtgga acacatcttc atttcttcta cggcatcaac aatccaagcc
      181 gggtcttttg actgctggaa caacgcgatc tcggacgagg ccagtgcatt gtgatacgac
      241 gcgttccatg gttcgatctt gatcgcgacg gcataccacc gcaccgatgt tcgcaagtca
      301 cccgaactga gggcgctatt gccttgctcg aatgcgtacc aggcggccgc cggctgaacc
      361 gtcagcacga gaaggatcga taagatgagc ccgccaatca gcgcccggca tcgggtgtag
      421 ccgatcacca actgcggctg tgctggatcc tcgctacacc gcatccgtcc catggccagc
      481 accatcgccg ccgccaaaat gagcaacagc acaagggccg gttcgtggaa cacggagtcg
      541 accaaggcat gaatcagaat ggccgagacg atgccggcca ggccgatcat gattgcacgc
      601 gtcgcagatc gcgcttggtc gacgcatacg tatttgactt cctttaccca gagcacagcg
      661 ccggtcagaa caatgaacag ccccgctaat ccaagttctg aggcaatctg gaggtattca
      721 ttgtgggctg attctgctcg tctcccatac cgcgcaagtt cgccttcgat agggaaacgc
      781 gatttgaacg acacgtactt atacatgccc agtccgcttc cccatggttg gtcggctatc
      841 cgggccatag catttttcca aatatccaat cgggtatagg caaagtgatc ctgcgatgcc
      901 acttcaaggc tccgtgccct gattggattc ggaatgatga tgactgcgag caggccacac
      961 agcaggaaag gaatggctcg tcttctgaac cgcatgagtg cgaggaagaa gagtacaggc
     1021 acgagggcga gaagcgctcc ccgggagcga gcaagcacaa aggcgacaac ggagacaatc
     1081 aagagagccc acagagcggc tcgtttgccg ttgctgatat ccaggtcacc ggaaaagcag
     1141 aggagggacc ctgtcagcac cacgataccg gcttgatagg aggcaaagaa gttggggttg
     1201 aaaaatgtcc catgagcccg atactcatca aaaatcccgt attgaaggat gccaagcgcc
     1261 gcctgaagcg ctcccgtggc aagaataatg atgaccagga tccgcacgtc tcggaacgat
     1321 ttgactcctt gataggccag ttgaaagaag atcgcgtaca tggccaatgt gacgacccat
     1381 tgcacggcgg cgtgtttgta tggcgtccac aggaccgaga cggcggccaa cgtaaggaaa
     1441 cacagaatcc agggcgatac cgtgttccac tgcagagaca tggagccgtc ctttctcgac
     1501 gagaggatcc atgtcagaca ggaaaggaat agaaccgtcc gcatgacgga cacggggaaa
     1561 tgagtggtcc ccccttcgat caaaggggcg aagagtgtca gcgccgcaag aatatacgcg
     1621 acccgtgagt acggactcac cacgacagat cgggcctgca agatcaggaa cgcgggattg
     1681 atggatagag agggagttgc catgaatcag aatgccgcgc accaggtagt gcgggactaa
     1741 ctgctaaagc catctaaagt cagaacaact ttcgtaggat cacagtagtt ttgtttccct
     1801 aaagcgtcaa gaattttcac ttcttctacc cttgcagaaa cagcgaaggg gaggccttgt
     1861 cggcctcccc ttctttcctc aggagatcat ctcctgctga gtgactacac gtcgttctgc
     1921 gtattgttca aacgacgctc gtcattgatg taccactgat cacagttcgg atcgccatcg
     1981 atgtttccgt ttgcacccgc cgtgaacccg gacgggctag cggccacggt gaatccggtt
     2041 gtaggcgcaa cgttacaggt agccggcaca gatggcatcg gaaacattgc aggagttcca
     2101 ggaccaacgg ccggaccagt gatcgcagcc gcgtcccagg cataccaaaa tgtgtatcgg
     2161 ggcgtgccgg atggagccca gccaatgcca gaaaggttgg cctgagcggc gagataggtc
     2221 tgcggaacag tttctgcgtt gtacgctgtg gcggaggtaa agatcgcgcc cagactcacc
     2281 ttcgcctcag cctgcttcga cttcgactga taggcgatga agttcgggat ggcgatagcc
     2341 gccaagatac cgatgatcgc cacgacgatc atcaactcga tcagggtaaa acccttttgc
     2401 cctttgagcg acttacacat tgctactcct ccttgttgat ggttcatacg cacccgccct
     2461 attgccaaat ccgtgtaaaa ctcggcccgc tttgccgtca cggggtgcag tagcaggttc
     2521 ggtgccgaag gcggccacaa acgatcagcc agcggactgg cactttttca aggacttacg
     2581 gaactctata gactgaggcg ccgcaagcgt cgctaccgag ttctgctcag attcgccaat
     2641 tattgtcacc gcgtgctgaa gcaggaggac atcgcaggca cttcttagca agtggttcta
     2701 ctcagtggag gcaggacaaa ttccgcagac agcgcggacc ggccttcacc ggcgactgct
     2761 ggtctggaaa ggtgggagcg atttcgatac aaggccgacc gatttggata cgccttcctt
     2821 cttcaagaag gtcacgcttg gtctgcacca caaggcagga atctagcggg aagaaatgat
     2881 cgtttttcgc tcgttattgc gcgatcggca gagagggttt ctggccagcg actctgcgct
     2941 tggcctgctg gattcgttct tcttgtgcag gatcacccat gccttgctca cggaaccggt
     3001 ccatcaactt gtgattgctt ggatccagtt ccagggcgtg gagccaggct tcgcgggcat
     3061 cggaaaggcg ttgctgtttg gcgtaaatgt cgccgagatg ctcatagatc accgggtcgt
     3121 ctcccaccag tgcggccgct cgcttgagtt ctgcaagggc ttcggtcaat aaccccgatt
     3181 tgaaaaatgc ccatccgagg ctgtccacat agtagccgtt ctcaggtttg agcgcgaccg
     3241 cctgtttcgt cagggaaatc gcctggtcga ttttgattcc tcgatctgcg tagctgtatc
     3301 cgagatagtt gagcgcatcg gcatgatgcg gatcaagctg aatggcggtt tccatctctc
     3361 gtatgacatc atcgaagcgg tctagtttgt cataggccgt tccaagatta aaatgcaaat
     3421 cggcactctt gggattatga cggatgcctt cttgaaacac ttcggcagat ttgtcgtatt
     3481 gatcttgctg caggtaagct aatccttgca cgatgtggga ttccggctgt ttggggttca
     3541 gcgcgatcgc ccggcccaaa tgttccgtcg cttcagggaa cttcttgagc cggtagtaca
     3601 agacccctaa atgcaaatgc ccttcgaaaa acgtgggctc gagctgcaca ttgaatgtat
     3661 aggtctcgag cgctttttgc gtgtcttttg attcctcgta gacatagccg agataatctc
     3721 tcactttcag ctctgccgga cggaccttga gaatttctgt caaccgctcg atcgcttgag
     3781 gatattcctt ctgttcgccg tggatgaggg ccattcgaag ctgcgcatcc agatcggacg
     3841 gatcttcggc aatcatcgct tcaagttctt ttctggcacc cgaatagtcc ttggcgtcga
     3901 tatagagccg caccagctgg tggcggatat cccggttgcg ggagttgact tgttggaggt
     3961 atctcttgag cactccaacc gcgcgctctt tttcatggcg cgactcgtag atggcagcca
     4021 gtgccagata tgccggctcg aacgagtaat tggcggtgat ggcccgctcg aagctggtgg
     4081 tcgcctcttc caccttcccc gcctccagca ggacccgtcc cagctgatag tgcccgacgg
     4141 cgttatcggg cgatctggcg atcccggccc gcatcgcctg ctccgcttcc gccgggcgct
     4201 taagattgag gagcaagagg cctttcgaga aatacgcgtc tcccgattcg ggatcgagtt
     4261 ccactgcgcg gtccaatacg ctcaacgcct tgtcaccttt accgcctccg gcaagaatgc
     4321 ccgccatgtg cgtcaacatg cgcccgtctt cggcccggcc gtcggccacc tcctccgcat
     4381 aacgcaaggc atttggcatg tcgcccagcg aaaaatgcag gctggccagc cgtgctttca
     4441 cggacagagc attggggtcg gctttcaatg ccgcctcata ctccttgagg gcttgttcgg
     4501 aattttgcga cagctcggct tgatagccga gcatgaagtg gtaggccgca gtcgcgtcag
     4561 ctggacgcgc tgacgatgcc ggcttggccg gttgggcggt ttggaccgcc ccttcgggtg
     4621 atttcgacgg cgaagcgcag gctgcagccg ccaggaagga gagcggcatc aacgcgcaaa
     4681 gacaccgaag cgaccaggct gccgaggtgc tatacaattc catccgtctc atcggacgat
     4741 caacagcaat cctgagagga ctcgcaaaca ctcatcgatt atacgaagcg gtcatggcag
     4801 ggtcaaacga cttcacgatt gtcgaggctt tcgaactttg cgaatcggtc atggaaaaag
     4861 agttcctttt tccctatcgg accattgcga tgcttgctga cgagaatgtc ggctatacct
     4921 ttgcgctctg agttctgatc atagacgtct tcacgataaa taaacatcac cacatcggca
     4981 tcctgttcga tcgcaccgct ctcgcgcaga tccgcgagca tgggaatcgg gggtttcctg
     5041 gcctcgacgg cccggctgag ctgtgagagg gcaacgaccg gcacgttcaa ctccttggcc
     5101 aaggccttca gcgacctgga gatgtccgaa atctcctgct gacgcgactc ggcatcgctt
     5161 cgtccctgca tcaactgcag gtaatcgacg atcaagagat ccaatccccg ctcggccttt
     5221 agccgccggg cctttcccct catctgctgt acggttatgc ctccggtatc gtcgatatag
     5281 atcggggctt gctcaagacg gccagcagct tcagcgaggc gccaccagtc ctctttttgt
     5341 agtttaccgg tgcggagcgc atgagaatcg acgcgcgctt cggaactcag catgcggagc
     5401 acgatctggg gcttggacat ttccaggctg aagatgccaa ctgatgttcc ggcatggagg
     5461 gccgcatgcg cggccatgcc caagacgaga ctggtcttgc ccatgctcgg tcggccggcg
     5521 atcacgacca gatccgaggg ttgtagtcct gcggtcagat cgtcgagatc atagaacccc
     5581 gtcgggacgc ccgtgacctg ctccttcctc ttggagagct tatcgaccag atcaaggctt
     5641 tccttgataa tcgcgttaat gggactgaat gaccgatcaa gttttccctg cgcgatgcta
     5701 aagaccgagc gttccgcaaa atccagcagt tcatcgatgg gcgctgtgcc ttcgtaccct
     5761 ctggtcagga cttctgtcga cgtatgcaga agttcacgga gtaacgcctt gtcgcggacg
     5821 atcttgctgt ggtagcggat gttggccgaa cttggaacca tctgcaccag ctcggccaag
     5881 tacgccgcgc cgccgattgt ttcaagctcc ccctgggcct tcagccgttc ggttagtgtg
     5941 atgtgatcga tgacctctcc ggtctcagag agctcgagca tggcacgata aattttgcgg
     6001 tgagccgtgc gatagaaatt ttcttcgacc accaattcca tggccttggc catggcctgg
     6061 ttgtccaaca ggatggcgcc aaggacggat tgctccgcct cgacgttttg cggcggtaat
     6121 ttcggttgag agaggtcaat gcccggcatg gatttcattt cgattctttc cgccgtcgcc
     6181 gcgaccgtcc acctgccaat agagcggccc gcgtaagctt cttcatggcc ggacggtgcg
     6241 gacgtccgtt gcgtcgtcca tccgccccac cgagtttttc gaagtatgcc acatgatcct
     6301 gatccatccc tggcgcaccg atgacaatca atgcatgggc gccctgggcc tgggcggcct
     6361 caagcgcatt cgacaggagg gtgtgctcgc tgcctttgat caatacttgc accacccggc
     6421 gctgcgtgcg gcgtaaggta tcggcaattt cgatcaccgc gcgcatggat cgacgaagac
     6481 cggccacgac ataatcaagg cgtgggccat cgactcccgg ttgcggcatc tcgatggcgc
     6541 ggaagagatg atccacgtcc aaggcgaatc cggtcgaggg gagatcgcgc ccgaacctgc
     6601 cgatcaaatg atcgtagcgc ccgcctcccc ccagttcggc gcctacccca ggagcaaaca
     6661 cgtcgaacac gacgccgttg tagtagtcga acccccggaa ttcgccaaga tcgagcaata
     6721 tagaatcgcg gtggcctgca gcgcacaaga ggtgatacac ctgtgccagt cgatcgagcg
     6781 gagccgtaag ggccggctcg ccggcagcaa gaacgcgtcc ccgctccaac accgcctcgt
     6841 gcccggtcag ttcaggcgct tcccgaatgg ccttggctgc cgccttggca attcgttcgc
     6901 tggctaaaag ttcctcgagt cggggcaagt ctttcttcgc cgccgcctgt tccgcacgtt
     6961 tttgtccctg agcagagagt cctgaccgca tgagcaggcc cttgatgaat cccacgtgac
     7021 cgacggcgat ggtaaatgaa cgcaggccga tcgcttgcag acattcaatg aggagactga
     7081 tgatctcgta atcaccggtg acatcgtcga ctccgaccag ctccgctccc acttgaaaaa
     7141 tttcccgacc cctccccgca tgttccggtt catagcgaaa gaccgaggtc cggtaggaga
     7201 gccgctgcgg gagcatgctt cccgtcaggc ccatgccgat cgttcgagcc acttgtgccg
     7261 tcgcgtcagg tcgcaacaac agcgtccggc cggttgtgcg gtcaataaac tgataacagg
     7321 tttccaggag ctcggcttcg agaccaggag ccagcacatc caggtactcg aatgtcggca
     7381 gaataatctc gtcgtacccc cagcgcgaaa tctgcgagag cagttgagcc tcgatgcgac
     7441 gaacctgctt ggcggcatgc ggcagaatag tggccattcc cacagggaca agcgaacgtt
     7501 cacgggagga tggcgaatgc cctgaagaga ggcccgactt cggcggagca gtcaccatgg
     7561 tcaaccgatg gagcgcgtgt aggtggcggc ttggaacatt caggcttgga acgtctagga
     7621 cagcgctgca actttaacgg acacgatatc tttgctcgac tgaatggtgt tcagtacatg
     7681 cgccgggaag gcttgatccg agccgataat caacaaggcg ttgccgccac gcttctcaag
     7741 cgcgcattgc atacggacga tgttaatgtt gttgtcaccg agcaccttcc cgaccatccc
     7801 aatgactcca gggcggtcta tattatggat aaacaacatg tgcccttccg gcacgacttc
     7861 gactttgaac tggtcaattt cgacgacccg cggctccttc ttatggtaga gagtgccggc
     7921 gacgaggcgg gattgcttgc cggcttccac acgaacgcgg atcaggctcg tgaaatcgcc
     7981 cgcatcagta cttttcactt ctttgacttc gataccccgc tcttttgcga cgatgggcgc
     8041 attgacgtaa ttcaccgggg actccatgat cggcgtcaac aaccccttca agacggcaat
     8101 ggtcagaggc gcaatagaga gagtcgtgac atccccactg tactcgaccg tgacgcgctc
     8161 aagcccgcct tcacagagtt gcgtttccaa ccggccgagc tgctctgcca gcgagagata
     8221 gggctgcagc ctgggcagca actcgggcga taccgacgga atgttgaccg ccccgcgggc
     8281 aatccccttc gtaaaatagt cgacgatctg ttcggcgatt cccaccgcga cgttctcctg
     8341 cgcttcagtc gtctgtgcgc cgatgtgagg cgtgcaaatg aagttgtcga gggacaacag
     8401 cggatggtct gccttgaccg gttcttcctc gaacacatcg aatgccgccg ccgccacacg
     8461 cttgctcttg agcgcctcgt acaaatcgcc ttcgtggatg atcccgcctc gcgcgcagtt
     8521 gacgatcatg acccccggct tcatcgtctc gatcgcctgc gcgttaatga gattctttgt
     8581 ttcaggcgtt aacggcgtat ggacggagat gatgtcggcc cgtcgaaaca gttcggcgat
     8641 ggacagcatc tcgaccccca tcttctccgc gcggtcggtc gcgaggtagg gatcataggc
     8701 gataacgcgc atcccgacgc cctgggctaa tttcgtcagg tggctgccga tttgcccgac
     8761 accgacgatg ccgagcactt tgttgaagag ctcgacaccc atgaatttat ctttttccca
     8821 cttgcccgcc ttcacggacg tcgtcgcttg cgggatacgg cggctcatcg cgcagatcat
     8881 ggacatcgtg tgttcagccg tcgtcacggt gttcccgccc ggtgtgttca tgacgacgat
     8941 tccgcggcgg gtcgccgccg gtgtatccac gttgtccagg ccggagccgg cgcggccgac
     9001 gactttcagc ttttcagcgg cggcgatcag ttcctcggtc actttcgtgc ccgagcgcac
     9061 gatcagtcca tcggcatcct tgatctccct gaacagttcg tctttcggca tcttggactt
     9121 caccacgacg gtaaaaccgg cccgttcgag aagttcgacg ccttgcttcg agagactgtc
     9181 gctcaccaga attttcatcg tgtctgccta tttggccatc aatatttctt gcgccttcgc
     9241 cacgccactc ccgagtttca ccgggtggcc gagtcctttg agcaccattt ccgtcgccgc
     9301 aatggccgta atgacgtcga accggtcggc gtatcccatg tgggacaacc ggaaaatctt
     9361 gcccttaaga tggtcctgtc cgccggcggc ggtaatgcca tactgcaccc ggaggttctt
     9421 atagaccgct tgtccatcca cgccgtcagg cgctgacacg gccgtcaagg catcgctggg
     9481 cgattccttt ggaaacaagg caaggcctgc cgctttcatg ccttcccgca tggcttgagc
     9541 caactgtccc tggcgggcga aaatcttgtc gaggccttca gccttcaaca tcttgatcac
     9601 ttcctgaagc ccgatgatca atgagaccgt cggggtgaag gccgtctgat tcttgacttg
     9661 attctctcgt tccttcttga aattgaaata gaaggccgca ttcttggcct tgtccgccag
     9721 agcccatgct ttgtcgctca cactcacgaa ggcaaggccg ggcggcagca tcaatgcctt
     9781 ctgtgacccg gtaatgacaa catcgagtcc ccacgcatcg gtcttgatat cgaacacgcc
     9841 caatgccgtg atcgcatcga caaccagaat gacctgatca tgggccttga cgatctcgcc
     9901 cagcgccttc acgtcgtgcg agacgcccgt cgaggtctca ctcgcctgca catacacagc
     9961 cttgatggcg ggatcctttt tgagggcgtc cttcaccagt tgcggattga tcgcatgccc
    10021 ccactcaact ttcagttcga tggcctgaac gccgaacgtc ttgcaaatct tcccccagcg
    10081 ctctccaaat tttccgccgt tgatgcaaag agccttgtcg cctggcgaca ggaaattcga
    10141 gacggagccc tccattccgc ctgtgccgga ggcggccagc atgagcacgt cgtttctcgt
    10201 ttggaacagc catttcagcc cctcccgcgc ctcggcgaaa atcgggtcga attcgggcgc
    10261 ccggtggtgg atcatgggcc gggccatggc cagcaacact tccggcggga caggggttgg
    10321 accgggggct agcaggtacc gcttgagcat tgacgactcc tcctggaatg gaaatgcggg
    10381 gaatgcggct ctgagaaggg ccgacgataa caccgccctc cgaagactgt caaggcaacg
    10441 agtgacgctg acgatcgtga ttttcgatgt gttgagaaat cgacggcgat gtgacgatgc
    10501 aacggctgag ggaggcctct cctacgaaag gatggcctcg ctcatgctgc aattccttga
    10561 caactttccc gcctatggat agtctaactg cacacatgtt gcatattcga ttccacaatg
    10621 gtgtcttggg caggctgctc aagtctcaag caggttttct cggtctcctc ctgtgtcttg
    10681 caccgtctcc tggactcacc gagacgctga cggactccga catagcagct caactcgccg
    10741 aagcgccgcc tgtcatcaat tcaaccgacg agccggccat tgatcctgcc gacgaaccgg
    10801 acgccaatct tgtcccggtc gaaaccgagc tgccccctga cagtacagca tcggccacat
    10861 cgaagccgga cagccgcgtg cctcgggctg aggacctcct ggtcatcaca ccgccgcagc
    10921 agtcatccac cgcgcgtttc accgacgcat tcgatgcccc agctgaaacg gcggaaacgg
    10981 tgccgtccct cagcggtctg acggagccga gcgaactcta caatgtgcca atcgtgatgg
    11041 attcctcggt gcaaagtcac attcgatact tcaatacctc catccgtaac cgtttcgagc
    11101 aatggctgct ccggttgagc cggtatcgac ccctggtcga gaatattttc gccgagttcc
    11161 acctcccgag cgacctcgtc ttcttgtcgc tcgtggaaag cggatttaat ccatacgcct
    11221 actcgcgcgc gaaagccaca ggaccatggc aattcatgaa aggcacggcg aaagtctacg
    11281 gcctgcgcgt ggatgcgtac gttgacgaac ggcgtgatcc gattaagtcg accgtcgcgg
    11341 cggcccgcta tcttcgcgac ttgtacgatt tgttcggcgc ctggccgttg gccatggcgg
    11401 cctataatgc cggcgagggg aaggtcatgc gggcgctgca gaaggcccaa gccgagagct
    11461 ttaccgacat ctcaaaaacc cggttgatcc gtcgtgagac taaggagtat gtgccccggt
    11521 tcatggccgc aacgatcatt gctaaaaatc cgaaccggta cggctttccg caagaggcga
    11581 gtgcgcccca tcaattcgag gaagtcgtgg tgaccagacc gctgcacttc catgccatcg
    11641 cgaacgccac aggcgttccg ttctccgaac tccgactact caatccggag cttcgtcgtg
    11701 atgcgactcc gcccggcgac gatgcgtatt tgctcaaagt tccagtcgga acgaaggcca
    11761 aggtggaaca gctcctcgat cgcgtcccca cctataagtt cccccgggag gcgaaaccgc
    11821 agtttgcttc ggccggctct cgctggtaca aagttcgcgt gggggacacc ttggagaaag
    11881 tctcgcgccg ctttcgtatt ccgctgaaga ccctcaaaac cagaaacaat ctcaccggcc
    11941 cgacgatcag accgggcgac tttctcgtca tcggccgcta aaaaggcgcg cgccctcttg
    12001 aacggcattg ggtgtgtctc cgacgcaccg ctacggcttt ttttcgggag aggcaggaac
    12061 tgcagggggt gactggggag aggcctcctt gggcttcacg tcgagcactt tggttctgat
    12121 cgtcgcttcg ctggcgaacg gtgaggtggg ataagtcttg atcagctcct gataccgggc
    12181 cagcgctcct tcgggacgcg actgcgattc ttctaaccgt gccaattcaa acagcgcctg
    12241 atctttgagc aaggctcccg ggcgctccac aatggcagca aacgctttgg ccgcttggtc
    12301 gcgctctccc ttcaagagat aactataggc caggcgttgc tggacaagtc ccgcaagggc
    12361 agaatgactg ccatacagga gcatgaagcg ttcatacgtt tcaattgccg ccgggagatc
    12421 gttggcctgc gcctgtgtat tgccgagatg atacatggcc aacggcgccg ttgaggtacg
    12481 aggatactgc tcgatgactt cccgatattt cgccagggcc tgcgttaaca gggtatcggc
    12541 atttttggga tccgccgtgg aacgggccgt cagcagccgc gcggcttctc gctcgagttc
    12601 atgggctttc tgggatgcct gccgatccat ccagagtaca ccgcccaccg ccgcaacggc
    12661 gagaagcaga acggcaaagc cgaccagcaa cggaagccgg tattcctgca ggcggtggag
    12721 ctgatgctcc aatccactaa gcaggtgtgc ttcgtcgact tgcagcgtct tggcgggaac
    12781 cttgatgcgg taactcatcg gggcatcaca ccacgcacga acccatttcc tcgacctatc
    12841 ctcgcgcctt atactaagga agttacgggc ttgtcaatgt aatgagggcc ggcaaaacct
    12901 gcctgcagcc atgactcggc cgtcgcggaa ccgtcattca tgatgtttga agactacctc
    12961 gcgcagctgg cttcgaagca tctgacgaga cggttgctgc cgctggaatc aggcacggga
    13021 cctgtcgtgc gcattgccgg acgcgacgtc ttactgttcg cctcgaatga ctacctcgga
    13081 cttgcaggtc atgccgatgt tgtcgatgcc gcggtcacgg ctactcaaac ctacggagcg
    13141 ggagccggcg cggcccgatt agtatccgga tcgctgcccc cgcatcaaca tctcgaggaa
    13201 gcccttgccc ggttcaaggg cacagaatcc gcactcacgt tggggtcggg ctatcttgcg
    13261 aacatcggcg ccatccccgc tctgatcagc tccggcgggt tgatcctcgc agaccgcctc
    13321 tgtcatgcca gcttgatcga tggctgtcgt ttgagcggcg ccgacgtgcg aatttaccgg
    13381 cacaacgaca tggagcacct ggccagactg ctctcgaaaa gacgcgtcgg ccgccgaacg
    13441 ttgatcgtga cggacggggt cttcagcatg gacggcgatc ttgctcccct cggtgacctg
    13501 gttgctcttg ccgaggcata tcaagccgac ctctacgtgg acgatgccca tggcaccggc
    13561 gtcatgggga cacatggacg cggaacgatc gagcacttcg gcctcgaaaa gcgcattgcc
    13621 ctgcacatgg ggacgatggg aaaagccttg ggatcaagcg gcgcgtttat agccggcacc
    13681 cgccatgcga ttcagtatct tttgcatcgc tgtcggtcat tcatgtttgc cactgcgtcg
    13741 ccgcccgcct ctgcggcggc cgcgctcgcg gccctgcgca tcatcgagcg agagcctgaa
    13801 cgacgccgtc gcctgtggga caatcgtcaa cgtttgtttg aaggactgcg gcgattgggc
    13861 ttcaccatgg cccccagtgt gtccccgatc atgccgattg tgatcgggcg agcagatacc
    13921 gccctccggt ttgccgaaca cctgctcgcc aacgatgtct tcgctcccgc cattcgcccc
    13981 ccctccgttc ctgccgatac cagccgtatt cgtgtcacgg tgacgtctga acacacgccg
    14041 gcgcagattg atcaggcgct cgccgcgttc gaacgaaccg gacaagacac tcgcgtactc
    14101 tgacctcgtc tgaatcaaga gatttgattg ctttcccatc aacttgtggc atcatcacgt
    14161 cctgtattga ccgccgccct cgagtaacgc ggatcgtccg actaaggagc tgtcttcatg
    14221 gatatatcaa agctgctgac cttttcagcc aaagaagggg catcggattg tcacatcagc
    14281 gccggtgaac caccaatgat ccgcctccat ggagacctca aaaaactcga ccatcccccg
    14341 ttgaccgctg aggaaacgca tgcgctcgtg tacgacatga tgagcgacgc gcaaagaaag
    14401 aatttcgaag aaaagcggga gtgcgatttc tcatttgagc tgggcgacat tgctcgattt
    14461 cgcgtgaacg tcttcgtgca aaaccgtgga ctcggcgccg tgttccggaa catccctacg
    14521 gaaatcattc cgatggaaaa gctcggcttg cccccgatcg tccgtcagct ctgcgataag
    14581 gaaaagggac ttattctcgt cacaggtccg accggttccg gtaagtcgac gaccctggcc
    14641 gcgatggtgg atttcctcaa caataccttt gagggacaca tcatcaccat cgaggacccg
    14701 atcgagttcg tccataagac gaagaagtgc ctcgtaaacc aacgcgaact cggtgtgcat
    14761 actctatcct tcgccaatgc gctcaagtcc gcgctccgtg aagatcccga catcatcctc
    14821 gtgggtgaaa tgcgcgatct ggagacaatc cagctggccc tgacggctgc cgaaacgggc
    14881 cacttggtct tcggcacgct ccacacctct agcgcaccca aaacgatcga ccgcatcatt
    14941 gacgcgtttc cccccgcgca gcaagcacag atccgcaccc agctctcaga agcgttggaa
    15001 gccgtcatca cacagacgtt gctgaaaaag aaagccggtg gtcgcgttgc cgccctcgaa
    15061 gtgatggtcg ccaccacagc ggttcggaac ttgattcggg aaggaaagct ccaccagatc
    15121 ccgggcatca tgcaggccag tcagaaagac ggcatgcaga cgatggatat ggcgctggtc
    15181 gagctggcca cgcggggcgt cgtgaccaag gcggaagctc agtcacgcag catgagcccg
    15241 aatcttttcg gcggctcggc cttaagcgga gccgcataag tcgcgtacga gaccaaggga
    15301 gttacgatgg atatccgcac gctgctcaag gtcatggtcg atcaggaagc ctcggacatg
    15361 tatctgacag tggattcacc gccgtcttac cgtgtccatg gttcaaccca gccggccaac
    15421 acaccgccgt tcaccaatga acaattggaa gcgttggcat tggcactgat gcgtgggcag
    15481 caacgcagtg agttcgaaga gaagatggag atgaatctgg ctctctacta taaggagttg
    15541 ggccggttcc gggtgaatat attccggcaa cggggcaacg tcgggctggt gttccggcat
    15601 atcaaagcgg agattcagac ggtggagcag ctccgcctgc cacagatcat gaaagacatt
    15661 gccatgacga agcgcggtct cgtgctcgtc gtcggcgcca cggggtcggg aaaatcgacg
    15721 actctcgccg cgatgatcga tcatcggaac tccgtccatc ctggccacat tgtcaccgtc
    15781 gaagacccga tcgagttcgt tcacaatcac aaaaagtcgc tgatcactca gcgggaagtc
    15841 ggcttcgata cgctgtcatt tcagaacgcg ctcaagaaca cgctgcgcca ggcgcccgac
    15901 gtgatcctca tcggtgaaat ccgcgatacg gaaaccatgg aagccgccat cacgtttgcc
    15961 gaaaccggcc atctctgtat cggcactttg cactccaaca acgccaacca ggcaatcgag
    16021 cgtatcatga attttttccc tgttgagcgt catgcgcaaa tctatctcca actctccctc
    16081 aatctccgcg cgatcgtgtc ccagcggctg gtcccgtcga cagacggcaa gcgcatccct
    16141 gcgctggaaa tcatgatgga tacgccgcgc atcaaggatt tgatcaagaa ggccgaggtg
    16201 gacacactca aggaagcgat ggaacagggc atcgaggagg gctgccagac attcgactat
    16261 gtcctgttgc aactctacaa ggaaaacaag attaccttgg agcaggccct catcaacgcc
    16321 gacagcgcca ataacctgcg tttgaagatt aagctcgaag ggctcaaagg agatgacgcc
    16381 atcaatgccc ttctggacaa gggccctcat gccacgggcg aaagtccgtt caaaatccag
    16441 ggcgggggac cgggcaacgt caggccgatt cgcaaactgt aacaaatacc cggcctgcgg
    16501 tcttctcgct actgctttcg cgagtcttcc gccgttcctt gttgttcgag ataagccgca
    16561 atcttttcca gagccaacgc gctgagcttt gctccatacc aggtcggcat cgtctcaggc
    16621 ggataaccag gcacgacaaa cacgcccggc tccaacaccg actcgaccac atattcgtgt
    16681 acggtcttcg cctttccgcc atacgcggga tcccgtaacc gttgcggccc gacagttcca
    16741 agtagtagcg gcggcccgac ccgtccgtcc gctcccggaa tacctggaat ctgatggcat
    16801 actgggcagc ctgtccgagt aaagattgcg gcaagcggct cttcgccggt cacaagggga
    16861 accatctccc gtgcgagcag aggtaccggg ggagccgtct gcacggctgg atcgcgcgca
    16921 gaaaagtagg cccagaccaa accgatgagc agaacactca tcagcaggac attgaccgtt
    16981 cgatcagaca tgtgatggtg gacgatgaaa caggcaagac ccgagctggc agagctcccg
    17041 cttcagcgcc agcgacggtg gaacggaccg ggtcactggc cgctgaccgg catggcacat
    17101 cgaaagccga tcgttcggtc ctcgaactct ggttcagccg aaattctcgc cgtcacacgc
    17161 agggccagcg gtaaatccgc ccaagagccg ccacgcagta cccgtttttc accttgtgcc
    17221 gggccggttg gattcttctt tgggccgttt tgatagtagt cccggtcata ccaatccgat
    17281 acccactcgg cggcgttgcc ggccatgtga ttcaatccgt atgggctctt gcctccctct
    17341 ttcaggccat gacgcacgct catgccttcg agaccactgg tcaccgcggc taatgtgacc
    17401 gcttcactca cccagaggcc gcggttgtaa ttggcgatgt ccacgaaagg ttgcatcgat
    17461 ccccagggat aacgcctggc gtcggtgcct cgcgcggcct tttcccattc ggcttcggtc
    17521 ggcaagcgct tgccggccca tttacagtac gcatccgcgt ccgcccattt catgccgatc
    17581 gccggccggt cccccaccgt cgtcgcggct tcgtcgtccc aagtcggggg cgagtcgtgc
    17641 tttcccgcgt ccaggaactt ccgatacagc gccaaggtca cttcatactg atcgatgtaa
    17701 tacgcatcga gttcgacgat gtgttctggg cgctcgttcg gtaggccctc gttgctcccc
    17761 atcgtgaacg ggcctgcagg gatcaggatc atcggcgccc cgtcttttcc tttgaccgtc
    17821 tcgatgcccg gcggcgcgtc tttgggaggc ggtccggcag ctactgtcag cgcgcccgca
    17881 gcaagaagag caatgacccc agtgatcaag ccccaatttc ccatactccc tccatgactg
    17941 cctccgtcat tgcaaaacga cggtaggtaa gtctaccgag cttaggagac aaactcaagg
    18001 aaacgggccc accgccgact cctcctctcc catgctacgc cggagggaac tgagatcact
    18061 tgtcctggag agagaagcca aacagatttt tgcagcaccg aagagagtgg tcatgccatg
    18121 cgacgaaacc agacgtatgt tggcgagtag actttggcga tataacgcag aggctgaaga
    18181 cgtatccagg ttgtcgctgg ggggagggaa aggaagcgcc gcttaatgaa tcgccgcgga
    18241 acgccggttg accgattcga tgatttcgtc ccgcagttgc tcgacggccg cctccttcga
    18301 tatgaccgtg gtggcgccgg ccgccgtcat ggctttccgc gtgtcgtcgt tggcgtagac
    18361 cgacagtcct actacgacaa tgtgtggata gaccctctta atcaaggtgg tcgccttgat
    18421 gccgtccagt cgaggcatgt taatgtccat gacgaccact gatggcttca acagctgcac
    18481 ggattctaac gcttcctctc cattgcaggc ttcccccaca atttcaaagt tgggataggt
    18541 ggcgaggatg cttcggagcg tttgacgaac actctcaaca tcgtcgacga gcagcacccg
    18601 gataacgtcc gttcgcgtgg atttgcgatt gacacgacgc gacacttgag acatttgact
    18661 gcccccgttt atgacggtga atctctgaaa cgccggcttt cgagtggaac cattagtgtt
    18721 atcgaaactt gtacggtccg ccacctagga caatgcctag ccaaatgaac ctgccgtttc
    18781 ggtttaccaa aaggacggat gctcaatagc ctttctcctt attctaaggc agccttggac
    18841 caatctgatt gtgccatcga atccgtcaga caaaatcgga atcgagcagc ggcggaaact
    18901 tatctttctc cttctcaccc ttgtactggg gataccccta gtataagcct gcctggtccg
    18961 cttcttgcgc tctccgggca acattattgc ttcctgcctc tatgaaactc gcatcagaga
    19021 gatggatgta ccgctattca tgagcaccca tcgttataag gggcatacta tcgccctcat
    19081 tcccagtatg gaaggcctga tgtgggcctg ccaatacgtc atcatgaagg ccgatcaaac
    19141 cgagatagcc ggtttcccag atgggaacac ctacgacgat cgacaacaag cggaattggc
    19201 cgctcttgcc aaagcaaaat ccttgattga cgaatccgct ctcaacaaag atccattggg
    19261 gagcggacgt tagagaagcc ttgtccacgc atgtgacaat ccttccactg tgtcacagaa
    19321 gcaaaccaag taggcagagc agatctatcc tcgcgttgcc ttgctctcat ctccgacact
    19381 gactccatcg atgcaaggcg cttccccagt ggtgtgttgg acaagaaggc gtatcgtgtg
    19441 tgcatggatg acgctgacac acctctcgta cacacggaag gagtgcgcga ttgaagagct
    19501 gatgcaagag tgttcctcgt atgcgtggaa tcaggttttt ttggagatcg atcgattgag
    19561 tcgctccggt cagctatgct cgctatataa aaaagacggt gactatgccg tgaggctccc
    19621 tcgcgccgca taatcgttgt gcaccaacct gatcgacact cgcaacagtg gtaggaattt
    19681 atggcgattg gacccgacgt actcgtcttt atctcggcat cgagagcact tcttcaatcc
    19741 atccgacggc agccgctcaa tgaggaagaa caggcggaat tggcctcctg cctccaagag
    19801 ctgctcgccc ttctaccccg ggactacggg cgacacgcag cataagcgcc accgccaaaa
    19861 gacggacgcg caaaagcttt agacaaagcg gcagtcgtgc caattccagt ccgtccaggc
    19921 ctgctatatc gccctctatg ttagttatgc catccctgcc ctcagatttc ttcccctgaa
    19981 tagggcttga agttgcgctc acgtttgcga aaaaggcgtt atagattgtc caagcacgac
    20041 acccgtgcta gaacacgatt ggtaaatttt tctctcaagt gcacacaacc tacggaggac
    20101 atgtatgtgg tattttcggc gacggatgtg gtgggtgttc ttgctgatcg gatgctcaat
    20161 gcccaataca ttggttcagg cagaaatgta tgtcgccgga caggtgggtg tgacacttcc
    20221 gcaaagtctc agcaacgtcg aggtcagcga cccctccatc ggcctcccgt ccggaagcac
    20281 gttctcaaat ctcgatctcc aaaattcggt gatgtatgga gcaaagctgg gctattattt
    20341 tgagtcactg aagtggttag gggtggaaac tgaggtgttc aatacgactc cgcacgtcaa
    20401 gcaacaaccg attaccgtca ccgtcccagg atcgccttcg ttcacgatca accccgccgg
    20461 ttcgtaccta cgcgtattga cctgggctcc gatcaacctc gtcgtgcggt atcaaatggg
    20521 aaagtttgag ccctatgcag gagtgggcat gggcatcttc ttttcgcggc tcaaagacgc
    20581 cggttcaaac gattcgacgt cgagcaccag cgttggactg aacacgcaag tcggcttgcg
    20641 ctatcgcgtc acgaacaacg ttgcactatt tggcgagtgg aagttcaatc acgcacgact
    20701 cggctttgac gccacgccga atgcgtttac gaatgcagat gcgacctata atgcgcatca
    20761 cctggtcttc ggcgtggggt atcacttcta agatcatggg catgttcaat aacatagact
    20821 ttactcagga tgacataacc ggctttaagt aaagccgatc gaagaaaaac ccccactccg
    20881 ttgtcactga gcggtcttcg gtgcaacgga gtgagacctc accgcactga tatttgtctc
    20941 tccgaacttc ctacccctgc ctgatccctc ggcaacccag gccatcccct agtatgactc
    21001 ggaagcgacc tatgctgtag tgaaagcttc accagcagcg ctcagcgtcc ctttcccatg
    21061 gaggtggaag catgaaaaat tgcgcggttc ctctatgcac ctgccagatc ccagaaggaa
    21121 aggagttttg tagtgacgaa tgccgcaatg gattgccgtc gggcttgtgc cattgcccac
    21181 acaaagaatg ccgggggcat gtacagccga acccctccga ccgcggccgg tagcccatcc
    21241 cgttcttgcg attgctttga gattccgtcc atgggctata gcccttccac ttgacccgct
    21301 tggaggtgtt atggggcgcc cacagtaaag actggtcact tccttcctac ctgcctttca
    21361 tagttgcttt ccaaccttca cacctctgta cgatgctcga gtcttgccag gccgctagct
    21421 ccccgcttct gccaacataa tagcttcagt gcccgtgtga gacagattca tgcggacatt
    21481 cttgaggcac ggcctttgtg gtagttttgc aatacaaagg agttctgcgc tattcattgg
    21541 gcgggaactt gggacacatg acttgtcatc cgaggtgatt ctggtagtga gggcgggtcc
    21601 gaccgggctc cgcggagctt ttgcatgagg cacgcgactg caaacgcccg gtggaaagaa
    21661 ccgatgcgcg ggctcctcat cgttgttgca tgttgtgccc tggtcagcgc ctgttcaccc
    21721 tctgtcttcc cttacaaggc gttggacggt gtgaatcaag actttgactt tgcgggctgg
    21781 caattgctgc aggaaccaca tccacagcag aaggtgcaac tgggagggcg tatcgttcag
    21841 gctgaaatga aggatgacat ggtgaccatc acagcaacgc agctctcgat acccaagaat
    21901 cccacacatg agccggagga ggacaaatca agtggtgagt tcctcatcca gtatagcgct
    21961 gtcattgatc ccctcgttct tcgttcggga aacaagctca ttgttgtagg cgaaaccggc
    22021 agcccaggac agatcgaggt cgaaaagaaa gttcggctct taccaaagat caccgccttg
    22081 tgcatccatt tttggaatac cgatggcaac gggattgtgt tacgaggatc ttcaagtgct
    22141 ggcacgaagt ttctcgctga actgacatac tgcaagacgg cactgtgagt gtgcgcgcca
    22201 tatcgaaagg cttcaggccg tttccccata gttctcctct cgaataagcc gcttttaagg
    22261 tcgctttaat ctctacttaa tttcgctgat ttatttgtaa ccgttggagg agccaatgcg
    22321 ttggcgagcc tattggtcgg cactgggaat ggcggtcgta tcgctgctgg cgagaagcgg
    22381 agctttacac cggcccctta agaggaagta gccgctttca atggatacga ttggatagtt
    22441 cggaggaatc atgtcgcagc gggttaagac taactatagc tacaaaggct ggaatatcac
    22501 tctggtgatg accctcgaag gatacatgtg gacctgtcag tacatcatca ggaggtcggg
    22561 caggacggtg atggatggat ttccaggaag cacatatctc tcgcgcgaag aagcggaaga
    22621 aacggctttg agtaccgcca aaggtctgat cgatcagtgt cagctggaca agaatccgct
    22681 aggtatttaa ctgaggtcat gagtcactgg aattgaaacc ggctcctctc gtaactcggc
    22741 gttgacccag acacaacgag cgtcacgcgt gattctcaga cgaatgtcca tctcgtttct
    22801 tgtgagtgct gagaagtttc gccatttgtt ccgtatagcg ctcaagtaac cggtgttctt
    22861 ccttggaaaa cgggggactg ttgggcactg aagcaacgga gatgagatat tcacacgagc
    22921 gcacgtagtt ctcgacgtct ttgcgggggg ctttctgggg tgggcgcagg gacatcggtc
    22981 tttcacttgg tcacaagtgc tacagatcca ttggcacctg caaatcactc tagaggccgt
    23041 ctactatcgt gtcaacagga atacagcgag tcgccttaat ccgtcagatg ttttcctaca
    23101 ctggcggcgc tgtccgacag catcgcatga gtatgcggac atcgtgcaat agatttccct
    23161 cggcagtggg agtatggtga acgaatgtgg caaagactcc tgaaagcgac aaccgtgact
    23221 accttggcat ttcttctcaa gcgcctgttc attcggagca aatcagcgtt accctaccgc
    23281 caccctgtct ctcgttggcc tcgtcgttct tatcgcaaac catagaaatc gtgggaggaa
    23341 ggccagccga ggttactcga tccaccactg ataacagtcg ccccgaagaa tcgcttcgtt
    23401 acaacagtcg atcatcatga gcatgccaag ctgaaattga aacgcatccc ctctggcaaa
    23461 accacagtcc aacatgtcca ccgcatgatt gaacgccatt tgctccatga ctacggctgg
    23521 agcgccgtgg atacgcgtga ggacgatttt ccatgctcgt cgccaatcat ttccgttcca
    23581 ttcgacagtg cccgtgcgta cccgtttccg acgcgccatc tcccctccga gtcacaccat
    23641 taacatactc atcggcacac cttgttagtg gctgctctaa gacaggtaaa cttgtgcggt
    23701 tgatcacgta aagaatcagt gatccacgtt cgtcttctcc tcaattaagt gtatatactt
    23761 agtatccaga gcgggctatc attttttcgt ggagcgagac atacttttcg cgctcggctt
    23821 tgtgatgtac cggatctgag agttcaatcc tgtgcaaacc agggatccac gatcactgct
    23881 ggacggcggt ctgagcgtct atgggatcgc aatggatgcg agctgcttca tccctcagac
    23941 attcctcccg attatcctaa caagtctcct tactgggctc tttgcgttag tccgtgtgcg
    24001 aaccgtggaa atgagaacaa tggccatatc gcgaggtgat ctatgtactc ccgtttttac
    24061 ggttttacca cggagccatt tcaaaacacc ccggacctcg attttctctt cctcagtaca
    24121 aatcataaag aagcgttagc ttgcattgaa tacggcatta gacaacgtaa agggttcatc
    24181 gcgatcaccg gcgaggcggg agttggcaaa accataatcc ttcgctccta tctcaccaaa
    24241 gtcgatgcac agaaacagaa aacgatttat atcctcaatc ccagggtcag tttcgatagt
    24301 gttctcacga ccattctcaa agaattaagc gcggagcctg tcgctggccc tgcaacacag
    24361 aagctcaatc aactgcatga ggtgttgata gctgaatacc gcaatgaccg cacggtggta
    24421 ctcattatcg acgaagccca gaacatgcct atcgaaacgc tggaaagtat tcgcctgctg
    24481 cttaacctag aaaccgcaaa ggagaaatta attcagatgg tctttgtggg ccagccagag
    24541 ctggatacct tactcaaccg ctatgagttg cgccaagttc gtgagcgaat cgcccttcgg
    24601 gcaagaattg caccgctcac caaaactgag agtgaagact acattgatca ccggctcagt
    24661 ctggtaagag gagagccacg ggcggtcttt tcaaagcaag cgctcgatcg cattgtgaaa
    24721 tccgcaaatg ggattccaag aagactgaac attctctgtg atgccgtgct gatcgcaggg
    24781 tttgaatatc ggcaagcaat catttcagac aggattgcga aggaggcaat tgctgctttt
    24841 attggcttgt ctcagccccc tcgaagactt cagccgcggt atgccgtggg catgctgagt
    24901 gtggtgctaa tggtcgtctt ctatcttgtc tatctctcta ccatgccagg cattacggtc
    24961 agcgggccct cagaggatac ggtccaaaga aaagaacccc caaagcctgt tctgggaacc
    25021 atcggagcag atcaactatt cgcacgcgac cgcgatccag actccgtgcc gccagcacgc
    25081 ttggtcagtg agttcgaacc aaaagagggc aacgatctct ccccagagga gaggatggag
    25141 ttgagtgaaa cagccaggct tttggccgtt cttttggatt ctggaagggt tgtcctggga
    25201 cgagcacagc ccacgatcaa caatcccaga attggggaca aagggttttc ctcgagtgtt
    25261 tttgaagggc agctgcgggc agaatttcag acaagaactc gacatgatct acacaatctt
    25321 gccgttgccc cgatgccgga atccgccaaa cacttgctcc tccggctggc cttcttcatg
    25381 caaaaagccg tacacgatgt gcaagccgac atcaacaaaa agggaatcgg gtttaaaggc
    25441 ttcatccccg cgacatttgg aacgcaggtg gcctatagct tctcgaagga caccggctta
    25501 aagttgcggc agattggccc gcccggaacc gagccgcgaa accccgataa caaaccgaat
    25561 gaacaagagg agcaactcct ttacgccgtc cagaaaaatc atccccgggt tggagatcac
    25621 attgtagagc aacagctggc tgatattcgt ggtgttcgcg tcatgctccc cctcttttac
    25681 agcaaacaat gtctcgcctg tcatggcaac cccaagggac aggtcgatat ctccgggtat
    25741 ccaaaggaag gcttcaagga aggagattta ggaggagcca tttctattgt gctgtctgcg
    25801 cagaagaagc tcacaaagag cagcgaatga gtctctcgta ccagcaatca acacaatggt
    25861 atcagagtgc agtcgatcaa ctgacgcgaa tcgcggcggc gatgcagcgg caggaacact
    25921 tcgatttgaa gaggctgtcg tcgcttgcgt cggagatggt ggagtcgctt aaccggggtg
    25981 accagctggt agtcgaagct ctcgcggggt ctagcgggtc accattgatc acgaatctga
    26041 tcaatgtcgg tatcttggcg acgaaggtgg gggtagggct cgggtattcc gggatggagt
    26101 tggaacggct ggccctggct gggctggtgc atgatatcgg cctctttgcc ttcccgcagt
    26161 cactggttac taaagccggt cacctgactc ctgaagagcg catgctcgtc gagcaacatc
    26221 ccgaagtggg ttatcaagcg ctggggaaac tcggaacgac gcatgactgg ctggcggagg
    26281 tagtccgcca ggcccacgaa cggtggaatg ggcaaggcta ccccaataaa ctcaagggtc
    26341 gccagatcca agaattcgct caaatcatcg gtgccgtgga tgtctttgag gcactgatca
    26401 gtccccgtcc ttatcgtcgc cgattccttc ctcacgaggc cgtgcgcgag ctcgtggcgg
    26461 tcgagagaac ggcgtttcgg cgggaagtca tcaaggcgtt ggtcgaacag ctctcggcct
    26521 atccgattgg aacctcggtt cgtctctcca ccggtgagct gggcacagtg atccacgtca
    26581 atactcactt tccactccgt ccggtggttt tgatcggtga aggtcggaca gagggcctcg
    26641 cagcacggca agtcgatctg agtacgtcac cacgggtaac gatcattgag accgtcgagc
    26701 ctcctgcgat cgcgcgtata tcatttccaa tacgaactct ggcgctccct gtcacgggac
    26761 atccctttcc tgccgttctg tcgccaagga atcgggccgc tcttgatgca attgcgaatg
    26821 tgattcaaga ggtggtggag tcagtcgcgc ctgtccgtcg aggtgtcgac cgcctttcag
    26881 aaggggagac gacgtggccg cctcgacgca cggctgttca ggactcgttc attgaggctc
    26941 catcctccca ctctttgtcg aaccgtcaac aggcagccac gccgcaggca gtcattcaat
    27001 catcagaggc gagcgagcca ccgcttctga aagcgttacg tgcacttcag caagctcgag
    27061 cgaggtctgc gcagcccact cgagatgtct tggaaacagt catcctgagg gctggacacg
    27121 aggcgggaga gataggcgta tcggtcatcg aacatatcct caatgagttg gataggcttg
    27181 acgaacaatt tattgaagac gtaagacggc gggttccggt gatcgtacaa gcacttcaca
    27241 ccttacgaag tcaggagacg accggttttg tgaccgcttc tcacatcgat ccaatcgtgt
    27301 gcgaagttga tgcactgtac gatctggcga atcgtgtcga agcttccatg atgaccacgt
    27361 ttctccacgg tcttcgatcg ctcctacgat atgatctggc gaatcgtgtc gaagcctcca
    27421 tgatgaccat gatccgccac ggtcttcgct cgcttttttt ggcgcgcgcc gacagaaagg
    27481 cctcgacgct gcctcaatgg cttgagacgg tggaagcgcg agtgcagacc ctcattccaa
    27541 tggccgaaca gtgggtcaat attgggcgga cagaacgggc agacatcagt gagattctac
    27601 gagcctagcc cgacggacgc tccttcttcc tacccccaat accagtacgc cgccgatcca
    27661 acctcagaca accgaaccac acaagaaacg tggctagttt gccttgtctt ggtagcagcg
    27721 aggtgacaga ggaagatggg caggacggag aacaatgccc cttatttgat cttatcgggt
    27781 gacgagaagg ggccagccag gaaaactctt gatagcattg ccgatccacg actggttgac
    27841 ggcaggttgt tcgccattag tcagatatct caccttcgac gacaagctgt gcactcggga
    27901 ggcagcgtcc ttgtaacgcc gattcgtgtc ggcgatacga cggtagatct cgaccgcttc
    27961 ggcgatctgt ccggccgttt cgagggttcg cgctagaaag tactggatat cgagtaactc
    28021 ggtgcgagag gccgacttgt cgtccaaggc agcgcggaag atcttgatcg ccgtccgatg
    28081 ctctcccgcc gccctgtggc agagtccgat ctgtacctgt gctttgagtc gataggccgg
    28141 actttttccg gcgagttcca gcacttcgat cgcctctttg ggccggcctt ccttcctcat
    28201 tgctactccc ttggcataga aaaagggttc gggagagatg gcgggcgttg ctgggcgctc
    28261 cgggtgtgga ttctcgagcc actgcgccgg ggtgggttgc ggcgtcgggg cgtcgatttc
    28321 cctggtatca tccgggaggt tggacaacac catcagttcc tctctcgacg ataacggaag
    28381 aatcttgccc ttacttcgct caagcgcggc ctgcgcgacg atcttggatg taatgacctg
    28441 agcctgcagg ccaaagccat gggtcatggt cagatcgcac acctgattga tgagacgcgg
    28501 gttgcccttc gtcaaccgat gcaccaaggc acaggccttg ttggtgaaga gcgagggatg
    28561 tccgccggcg acgcgcaatc gatgatggat gaattgcccg gtttcgacct ccgaaagcag
    28621 ttccaagtga cagtcaacgg ccacgcgttg ggcgaactgc gtcatgtcga tccgctgcag
    28681 caacttgtgg aggtccggct ggccggaaag gacgatctgc agcttcagcg ttttcccgtc
    28741 gttcaaattg gagagcaggc gcagttcttc aaggaggtcg gttccgagac tctgggcttc
    28801 gtcgatgatc agaatgaccc gccggtgctg ctgggattcg tgagccagat attccgagaa
    28861 gacatagtag gcttcaaccg ggtccagttg tcgcttgctc aatcggaggg acaacagcac
    28921 ccaggggagg agatgttcga tgttgtaccg cgcgttcgtg acgaggccga tcgagtgctt
    28981 ggttccgtgc tctgcgatca gcttccggag gagcgacgtc tttcccatcc ccggttcgcc
    29041 ggtgagcacc atgaacgccg cctcgtggat gataccgtat tccagcgtgc tataggccgc
    29101 gcgatgcgtg gatccggcat acagaaagtc gctgtccggc aacagcgaga agggcttggt
    29161 gcgaagatgg taatacgatt cgtacattga gcgttgtctc cagtgagcga tagggccaga
    29221 atatgaggga aacgcttcgc tggccattcc ctttcaggcg ggggagtacc cactatgggt
    29281 gggaagggat tagaaggtag ctgggccgcc tcggctcaac gaggggtaaa actagaacgg
    29341 cttccattcg atacctctag cgacacctcg gtccattcgc ccatccacaa tgactttagt
    29401 aggccgctac gcttgaagat ttgtcgcaac aaaaaaaagg caagagatcc ctgggacctg
    29461 aagcggacgg aaggcttgag aggcccgata tggcgctgag atctggtgtc cccatcggga
    29521 gggtcaaacc ggacgaccgg aagccagcac tggtagtcat tagttcctat ccttccaacc
    29581 ctaaagagtg attgtctagg ctccacagag tggagcataa aaggaaaaag gaaaggaaac
    29641 cagcaccatg aaacacgtta tctcgagcag catcgagcga ttggtaagct tcgccttatt
    29701 caccttgggt attgccgccg tagccgtcgc tgccgctgcc ctcctgctta tcatcggcga
    29761 cacgttaggt cacgcatgag agggaaacct cacaccatgc gacacgcaat atttgacacc
    29821 ctgttcttcg cgttattgac cgccgcgact gccgccgttc tcttcagcat cggcatttct
    29881 ctcggacatg cccagtcatc gtggaaggat gggaagccta cgctctgcat cgcaaccaaa
    29941 ttaggcgacg tgtaatgctc ttgagagacc ctgcgcccca aatggagcaa gatctgaaag
    30001 cctttcgggg gaagagatga gtctacgtag tttacctggt gtccccaacg ggattagagg
    30061 gcttttgtag gcggtcaaag gatggctgga tagtgtcctt caggggtttt atacaagcag
    30121 cctgagataa ctctcactgt cacgccgctt tcccatcatt cgggccaatt ctccacagaa
    30181 cactgacgca tgctgtgtaa tttggcgcca gcatggtagc gcacctgatt atggctcatt
    30241 taacctcttg gatttattga taacgcacat caggaatgct attggtgctt ggcatccaaa
    30301 tggaataagg cccaatggca aaacgccgct cattcaggag acgtccgaac atgaagtcaa
    30361 ccgtaacatg gctggggctg gttggttgca gcctcgtcac attgtttata tcaacggtcg
    30421 aagccggcgg cctttcacta tataaaaccg ccacctcagg cgtagggcta gccagcgccg
    30481 gctgggcggc tcgcgcccag gatccagcaa cgttgctcaa gaatccggct ggcaggagcc
    30541 gcctcgatgg caatcagttc caaggggcgc aattactgga gtcgcggtgg caatccgatc
    30601 ggcatcctgc ctgccgcgag cacgttttac gtgcacggcc tgggcaatga catcaaagtc
    30661 ggttggggct gttttcatcg gggacgctag cgggtgctct ggttggaaac gagcttcatt
    30721 tcgggaagcg gtaaacacaa gccgatgaac ggaatgctag acacgggagc gggaggacga
    30781 aaatgaagga taccaagcca tttgcgggtc tcatcgtccg gccgttagca gcgtttgcaa
    30841 ccctgatggt cctgatgtcc tcaagtgagg caggatctct ggatcgcaca gtgctgccga
    30901 tcgcggagcc acaacggcca acctatacgg aactcgatgt tcgaaaagtg acgccgcctc
    30961 ctcacttcga ggtcaaagcg ccggagggag cgccgaacat cctggtcgtt cttgtcgatg
    31021 acctcggctt cgccgggacc agcgccttcg gaggaccagt cggcacgccg acgtttgacc
    31081 gcatcgcgca cgaggggctg aagtacaata atttcctcac caccgccgtc tgctcgccga
    31141 cgcgtgccgc gctcaag
//
LOCUS       DASSCQ010000039         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1282270, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000039 DASSCQ010000000
VERSION     DASSCQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282270"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1824
                     /locus_tag="VFH05_02620"
     CDS             142..1824
                     /locus_tag="VFH05_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677138.1"
                     /translation="MKLTAREIFMVVLVVCVGLFFAIPATTEAAKKSKGMVPGAATAL
                     QYAEAISKGDIVKTATLDFACQYRLLAPTTPGLTSSPGTSDASTERCWEDIKTAHNPA
                     LERVDTGMDVLWPTNGALPFFRDDLTHYPASAFVMDLVGLSPPGSGLHLEVVSTTPLP
                     NASFHLRPQAPLISVPATLVTLTVTYQDPLTAPITKAPGSYKWTNTVKPARRAVKKLN
                     TQWVVLSGLKRHGFPGDAAVVNLLVQDGGPAEGIQQEIIPFVTEKSKAIPGSVVWWEP
                     EDAPGLLVAAAARAAAFPNLRDRVALLNRILIVDPNQADALTVLTRDLYAVILREAMV
                     AHKLTVKDPSLAMAVNEQYWNVYAQGERLDLSLDMEMGGLDKPTTADYLYRLLPALRT
                     LAQVRPEQLDNRFRWGAALRWNLDQDLSIQVHEQLVKDVPIERKTAKAEALLQLAWSR
                     INKVAWNRNLDDRDVQAAYQDAEQAAALAEIPLDKFIAEYTKAYSLLFSPSRDNRAIL
                     GHLTEAKRWFDEIPGNNQQVWDFFIGADSLKSVLDADPIFQPLLASGASHKG"
     gene            1841..2536
                     /gene="aat"
                     /locus_tag="VFH05_02625"
     CDS             1841..2536
                     /gene="aat"
                     /locus_tag="VFH05_02625"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET6677139.1"
                     /translation="MVRLSRNPDDLTFPSVEQASPEGLLAVGGDLRFERLLEAYRRGI
                     FPWYSEDQPILWWSPDPRTVLYPEKLHISRSLKPSLRPGHFTVTLDTCFGEIVEHCAG
                     PRPQYPQGGTWITPAMMEAYVALHREGYAHSAETWLEGRLVGGLYGVAIGGAFFAESM
                     FTRVDNASKVALVFLIRQLRTWGFRLVDCQQSSPHVLRLGAEEIPRRLYIEQLASAVR
                     QRDRRGRWQFDEP"
     gene            complement(2569..3936)
                     /locus_tag="VFH05_02630"
     CDS             complement(2569..3936)
                     /locus_tag="VFH05_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6677140.1"
                     /translation="MIMPQQLDAYVNESRAQFEDFLAQMVEIPSISMDPSRAKDMGHM
                     ADLAEQVLTQAGAQAEIVETGGYPIVSAGWTTGGEHPTVTVYNHLDVQPAQEPEWRQQ
                     PFAFRNDGGIYRGRGATDDKGPALAALFGARLAIEQRVPINIRFLWELEEEIGSPHFS
                     AGLKNRAAIPRPDSVIVSDTIWIAKGKPAMPYGLRGLLGARLTLRTGSKDAHSGVTGG
                     AARNPLAELMEVAQACVNAKTGEVKIPGFYTDAVKPTREEIKSFLKSGFQVKRFKEAY
                     GFHSLRTDDPAEVTRRIWAAPTFEIHGLTGGYHGPGVKTVVPGHGELKVSMRLVPDQT
                     PEKVFALLKQYVAKLNPSVKVEREGMLHPFKGSFEGPYVECVRRAAKAGFGHEPAFIR
                     EGGSIGAVVTMQKTWKVPILFLGLSLPEHGYHAPNEYFDWGQASGGMKAFAHYFMELA
                     KMGKR"
     gene            4043..>4225
                     /locus_tag="VFH05_02635"
     CDS             4043..>4225
                     /locus_tag="VFH05_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677141.1"
                     /translation="MFKGLLRLLPLLMWAIGLGFAVVPPASAADPTAIRDILDEPSVY
                     HLRQVVLQGRVRNVQPL"
BASE COUNT          829 a         1288 c         1199 g          909 t
ORIGIN      
        1 gggtcggcca cgcccgctcc gccgtcaaac ccataaccgt tgcatgatgg tgctgagcgg
       61 ccgacccctt tcagatggga aaatccgcat ggtacagtat cggccttcgt ctctttgctt
      121 tcgtaaggag taaaacctcg catgaagctg actgctagag aaatatttat ggtcgtgctt
      181 gtcgtgtgtg tcggactgtt tttcgccatc cccgctacga ccgaggccgc aaaaaaatcc
      241 aaaggcatgg tacctggtgc ggcgacggcc cttcagtacg ccgaggcgat ctcgaagggc
      301 gacatcgtga agacggcgac cctcgacttt gcctgccaat atcggctcct ggccccgacg
      361 acgcccggtc tcacctcttc tccgggtacg tctgatgcgt caactgaacg ctgctgggag
      421 gacatcaaaa ctgctcacaa tcctgccctc gaacgggtag acaccggcat ggacgtcctc
      481 tggccaacca acggcgcgct accgtttttt cgcgatgatc tgacgcatta cccggcttcc
      541 gcctttgtga tggatctcgt tggcctgtcc cctccaggca gcggtctcca tcttgaggtg
      601 gtttcgacga cacctttgcc caatgcgtcg tttcatttac gtccccaggc accgcttatc
      661 tcggtgccgg cgacactcgt cacgctgacg gtcacctatc aggatcccct gaccgctccc
      721 atcacgaagg ccccggggag ctataagtgg acaaataccg tcaagcccgc gcgccgggcc
      781 gtcaagaaac tcaacactca atgggtcgtg ctctcaggat tgaagagaca cgggtttccc
      841 ggtgatgcgg cggtcgtcaa tctcctggtg caggatgggg gccctgccga gggcatccag
      901 caagagatca ttccatttgt gacggagaag agcaaagcga ttccggggtc agtggtctgg
      961 tgggaacctg aggatgcgcc gggcctcctg gtcgccgctg cggcacgcgc cgccgcgttt
     1021 ccgaatttgc gcgaccgcgt cgcgctctta aaccggattc tcatcgtcga tccgaaccaa
     1081 gccgacgcgc tgacggtcct cacccgtgat ctgtatgccg tcattcttcg agaagcgatg
     1141 gttgcacaca aactcacggt gaaagatccg tcgttggcca tggcggtcaa tgaacaatat
     1201 tggaatgtct atgctcaggg cgagcggctc gatctctccc tggatatgga aatggggggg
     1261 ctggataagc ccacgaccgc ggactatctc tatcgcttac ttcctgcgct gaggaccctg
     1321 gcccaggtcc ggccggagca gctggacaat cggttcagat ggggcgcagc cctccggtgg
     1381 aacctcgatc aggatctgtc catccaggtt cacgagcaat tggtgaagga cgtgccgatc
     1441 gagcgaaaga ccgcgaaagc ggaggcgctg ctgcaactcg cctggtcacg cattaataag
     1501 gtagcctgga accgcaacct tgatgacagg gatgtccagg cggcctatca ggatgcggag
     1561 caggccgctg cattggccga gattccgctg gataagttca tcgccgaata cacgaaggcc
     1621 tatagcctgc tgttttcgcc gagccgggac aatcgcgcca tcctcggaca cctcacggaa
     1681 gcgaagcgat ggttcgatga gatcccgggc aacaaccaac aggtctggga tttttttatc
     1741 ggagcggact cgttgaaatc cgttctcgac gctgacccga tcttccaacc actcctggcc
     1801 tcaggcgcat ctcacaaagg atgaccggcg ctgctcagcc atggtgaggt tgagccgaaa
     1861 tcctgacgac cttacctttc cttccgttga acaggcctcg ccggagggct tgctcgccgt
     1921 cggaggcgat ctccgctttg aacggcttct cgaagcctat cgtcgaggga tctttccctg
     1981 gtacagcgaa gaccaaccga ttttgtggtg gtcccccgac ccaagaacgg tcctctaccc
     2041 ggagaagctt cacatttcac gcagcctgaa gcccagcctt cgccccggac acttcacagt
     2101 gacacttgat acctgctttg gagaaatcgt cgagcattgt gccggcccgc gaccacagta
     2161 tcctcagggc ggaacgtgga tcactccggc gatgatggag gcttacgtgg cgctccatcg
     2221 cgaaggctat gcccattccg ccgagacctg gctggaaggc agactcgtcg gaggcctgta
     2281 cggagtggca atcggagggg cgttctttgc ggaatcgatg ttcacccggg tggacaatgc
     2341 atcgaaagtg gccttggtat tcctgatccg tcagctgcgg acctggggct ttcgcctcgt
     2401 cgattgccaa caatcctccc cgcatgtgct gcgtctaggg gccgaagaaa ttcctcgccg
     2461 gctgtacatt gagcagcttg ccagcgccgt gcggcaacgc gaccggcgcg gccgttggca
     2521 gttcgatgag ccataatgaa accatttgcg cagccgagcg cccttccctt acctctttcc
     2581 catcttggca agctccataa aatagtgtgc aaaggctttc ataccgcctg aagcctggcc
     2641 ccagtcaaaa tattcgttcg gtgcatgata gccgtgttcc ggcaggctga gtccgaggaa
     2701 gagaataggc actttccagg tcttctgcat tgtgacgacg gcgccgatgg aacccccttc
     2761 acggatgaac gccggttcgt ggccgaaccc cgccttggcg gcacgcctca cacattcgac
     2821 gtacggtccc tcgaagctgc ccttgaaggg atgcagcatg ccttcccgtt cgactttcac
     2881 gctgggattg agcttcgcca catactgttt gagcagagca aagacctttt caggcgtctg
     2941 atcgggtacg agacgcatgc tgaccttgag ctcaccatgt ccggggacga ctgtttttac
     3001 gccagggcca tgatagccgc cggtcaggcc gtggatttca aatgtcggcg ccgcccaaat
     3061 gcggcgcgtc acttcggcgg ggtcgtctgt ccggagcgag tgaaatccat aggcctcctt
     3121 gaagcgcttg acctgaaacc ccgacttgag aaaactcttg atctcctccc tcgtcggctt
     3181 gacggcatcg gtgtagaacc ccgggatttt cacttcgccg gttttggcgt tcacacaggc
     3241 ttgtgcgacc tccatcagct ccgccagcgg atttctcgca gcgcctcccg tgacaccgga
     3301 atgggcgtcc ttgctgccgg tgcgcaaggt cagcctggcg ccgagcaaac cacgcagtcc
     3361 atagggcatg gccggctttc ctttggcgat ccagatcgta tccgagacga tgaccgaatc
     3421 gggtcgagga atcgcagcac ggtttttgag accggcggaa aaatgcgggc tgccaatctc
     3481 ctcttcgagc tcccaaagaa accggatatt aatcggcacg cgctgttcga ttgccaagcg
     3541 ggcaccgaac aatgcagcga gtgccgggcc tttgtcgtcc gtcgctccgc gtccgcggta
     3601 aatcccgccg tcattgcgaa aggcaaacgg ttgttgccgc cattccggct cttgagcagg
     3661 ttgcacgtcc aaatgattgt acaccgttac ggttggatgc tcgccgccgg ttgtccaccc
     3721 cgccgacacg atggggtatc cgccggtctc gacgatctcg gcttgcgctc cggcttgcgt
     3781 cagcacctgc tcagcgaggt cggccatgtg ccccatgtcc ttcgctcgtg atggatccat
     3841 actgatggag ggtatctcga ccatttgagc caggaagtct tcgaactgtg cgcggctctc
     3901 gttgacgtaa gcgtccagtt gctgaggcat gatcatgtgt cattctccaa tgtggaaccg
     3961 cggattatac cggctgatcc ggcgctgtca aagtcactgt cacacgtggg aaagttgtca
     4021 agaaatgcta gaatgcccat cgatgttcaa aggcctgctc cgcctactcc cgctcttgat
     4081 gtgggcaatt ggtctcggtt tcgccgtcgt cccgcccgct tcagcagctg accccactgc
     4141 gatccgcgac attctcgatg aaccgagcgt ctaccacctc agacaagtgg tgctgcaggg
     4201 aagagtccgc aacgttcaac ccctt
//
LOCUS       DASSCQ010000040        53515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_441656, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000040 DASSCQ010000000
VERSION     DASSCQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 53515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53515
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441656"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFH05_02640"
     CDS             complement(<1..986)
                     /locus_tag="VFH05_02640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET6677142.1"
                     /translation="MTFTLTMSPSDLLLLLPEIFLTCWLCLVLIVDFSFPRLPKEHLA
                     YLSIAGLLGTLACLAWFDFSGISGTLFANMFVVDRMALFFKIFIVAATILVILASIEF
                     IHRFTFFRGEYYFLVVMSALGMMFMASANDLLSVFVTLEFSTFGFYVLVSYLREDVAS
                     NEAGLKFFILGVFAAGLLAYGISLVYGETGKLVFSDMKGVQATPGLIIGFLLIFGALG
                     FKIGAVPFHSWIPDTYHGSPTPVTAFLSIAPKGAAFAILLRMFFVALGTFKPTWVLLL
                     VAASILSMTYGNIVAIAQRNMKRLLAYSGIAQIGNVLIGLAAGTKMGNDAIL"
     gene            complement(983..2689)
                     /locus_tag="VFH05_02645"
     CDS             complement(983..2689)
                     /locus_tag="VFH05_02645"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET6677143.1"
                     /translation="MSDYLLLYILFAPFAGAIALIFASNRQPVLVRSIAASSAGISLL
                     ASLYLFYAYDPVRGGFQFIQKFEWSRQLGISLHLGVDGIGTPLVLASSILLFAGIFVS
                     WHIKDRTKEFYIWLLILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     LEMTDQEGLKQRDSVGFIFNFGANSKEYAAMKLVLFLSAWAVVALMGILLIYRYSGLN
                     TFDILQLREHAKLMNIPVLGTTLDKIIWILIFFGFASIAPLWPLHSWSPVGHAAAPAA
                     TSMLHAGVLMKLGHFSIIRVAFEILPDTTRELMPIAAVLCIFSIIYGGFVAFYAKDTK
                     YVIGYSSSSHMGYVFLGMAALNYIGLSGAVIYMFAHAMATGMLFAMAGWVYDQTHTRD
                     IPSLGGLSNRMPFIAGCFVIACMASVGMPGTVNFIAEVMIIVGSWDKYPFQVIVAVIG
                     IVITLAYLFKMMRGLFYGPMDQKYSHSHDAVAVVDRLPLLIMIVVSIGFGLFPMHMYN
                     VVRSGVDPLVAKITLVVPVAEASDEQAVPRQRTSRVTISPSPLVATKPGETPPQTVTG
                     LP"
     gene            complement(2694..4259)
                     /locus_tag="VFH05_02650"
     CDS             complement(2694..4259)
                     /locus_tag="VFH05_02650"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET6677144.1"
                     /translation="MLEQLDSTFPIITCLLFFPLVGAAVLWLFDDEDLIRTSALTITL
                     VELALAIFVLMRFVPDSPAMQFVEHVPWIPALGISYHVAVDGISVLFVGLTAFLTVLV
                     VIYSWDTIRYQTKLYMMALLALETTTMGVFVSLDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKYVLYTLLGSVFMLVGIALLDINYHNYATLHHMDQLYSFDLLELLAVPV
                     PLNQQLLIFWLMFLGFAFKAPVFPFHTWLPDALLEGPIGMAVVLAGLKLGTFGFMRFS
                     IPLLPDASKHPTVVSVMMALGLAAIVYGAIVALIQPDFRRLLAYSSISHLGFIVVGLF
                     ALNYQGLQGSLLTMINLGFTTAGLFFIAGFLYTRQQTTQLLAFGGMAKHTPVLATFFL
                     LIGLASIGLPGTNGFVGEFLILLGTFQAHWLYGGIAVTGVIFGAAYLLWYYERAVLGP
                     VGKAVRESIGDLHPRETVIALSLSAMILWIGLYPAPFLRMINGSVQALVDRLNHGTVA
                     AAHVAVPLSEKVH"
     gene            complement(4272..6239)
                     /gene="nuoL"
                     /locus_tag="VFH05_02655"
     CDS             complement(4272..6239)
                     /gene="nuoL"
                     /locus_tag="VFH05_02655"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET6677145.1"
                     /translation="MTDLLIKLIPVFPLLAVIANGVFGRRFWSHEWAHRLAWGSVGLS
                     FLSTILVFADIVRSETPHEVVAYQWIFGGNLSINMAFLVDPLTCVMLLVVTGVGFLIH
                     VYSVGYMHGEDGFTRFFTYMNLFMVSMLLLVMGNNYAVLFIGWEGVGLCSYLLIGYYY
                     DKVSAAKAASKAFVVNRIGDAGFLLAIFLVFINFKTLDYTKVFAQVGHLSPEMATAIS
                     LCLLVGAVGKSAQIPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHVIFDLSP
                     TAMGVVGLVGGATALFAATIGLVQNDIKRVLAYSTVSQLGYMFLGCGIGAYTAAIFHL
                     MTHAFFKALLFLSAGSVIHALSGEQDIRKMGGLSQKIPWTHRLFFIGTIAIAGIPPFA
                     GFWSKDEILAHAFTHHHYVLYGMAAIGALLTSFYMFRLTYLTFYGHSRMDHHTEEHVH
                     ESPMVMIGPLMVLGLLSLAGGFLGFPPDHGWLHQFLAPAAGASGEHEVSTGLVLSLMM
                     IASGIAVVGWGLAHYLYSMNPGAPERWAAKAGAVYTTLLNKYYVDEFYDLVFVEPTKK
                     FGLMLDWFDRVVVDGVVRAVARLAELGAEGSTWVEKHVIYGGINVVGYGNHLMAKQWR
                     QLQSGMVHHYAAIIVAGLFLLAVVIQLIVQR"
     gene            complement(6388..6690)
                     /gene="nuoK"
                     /locus_tag="VFH05_02660"
     CDS             complement(6388..6690)
                     /gene="nuoK"
                     /locus_tag="VFH05_02660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET6677146.1"
                     /translation="MVPLSAYVAVSAVLFITGLLGVLIRRNFIIVLMAVEIMLNAANI
                     NLVAFSHYLESMAGQLVALFVIAIAAGEAAVGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            complement(6748..7275)
                     /locus_tag="VFH05_02665"
     CDS             complement(6748..7275)
                     /locus_tag="VFH05_02665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET6677147.1"
                     /translation="MIPVFFAYFALMSMAAAVLTVALRNPVHCGLALLALLLHVSGLF
                     VLLNAEFLWAVQVVVYAGAILVLYLFVLMLLNLKTEERYFHSTYLYFVVPTLLGSLYV
                     LALLFKSPYGGAKGDAPARAVLQEGDTYAIGMKMFSEYLLQFEIVGIFLLGAIIGAIV
                     LAKTPKPLEDMRGKN"
     gene            complement(7289..7876)
                     /gene="nuoI"
                     /locus_tag="VFH05_02670"
     CDS             complement(7289..7876)
                     /gene="nuoI"
                     /locus_tag="VFH05_02670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET6677148.1"
                     /translation="MTVRNATKKFLQAALFYEIWDAMKVTFKHMFHKPITFQYPREQR
                     AIPDTHRGALGLLRYDDGQERCVGCDLCEAACPSRCIKVISAEDPERPLQRYATEFYI
                     DITKCVFCGYCVEACPVNALAMTKMYEFSTHDKRTLLFDKKRLYDIGERHLDDAKKYL
                     YAHNQEKNIEESREYRYYFPQSVIKSTQPAPKHLS"
     gene            complement(7884..10571)
                     /locus_tag="VFH05_02675"
     CDS             complement(7884..10571)
                     /locus_tag="VFH05_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6677149.1"
                     /translation="MGLKPATNPDVEAATIELSIDGKTVTAKDGVSLYDVISSTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKATAGMIIRTEGDDLFQARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSPVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVAKGDLPLACEFCGDCLAVCPVGAITNKF
                     SKYLYKPWQMKKTTTTCNYCGDGCQMYLETKDTEVIRVTSPLSWKNKWGDRADTAKGH
                     GGLCVRGRFGFEYIDSAARLKRPLLRKGNQLVEVPWLEAMHTIVDRFADIRKKHGPDA
                     IAGLITARCTNEELYLFQKLMRVAFRTNNLDSSARYGHMNFVRASKHALGVGRTPNDW
                     EDLTKAKAVVVIGSNLTETNPLTSVRIKEAIRVYKAQVVVFDSAITNLGKLASHPVLI
                     KAGTEGLVIDGLVKATVELDLVDEESVKKHPQAYAALKSAVASLSLEQLAAKTGVSVA
                     QFKEAATIFAESPRSIVLCAEGIVRQPDGYHNVLKLIDLAWITGKLGRPGCGVNTVTE
                     EANEQGAADMGVAPEFLPGLASFDDQAMRDKLAKAWDATLPAIGTGAHFMEILSRCRS
                     GEIRALYVVGENPLATLPASVEVRAALDRLELLVVQDPFLTETGNMAHAVLPATTSAE
                     KDGTFTNLEGRVLRVRQAMDAIGESVPDWQIMTSLANGLGCQWEYDSANDVQAEIMKL
                     LPGYYNLGQPKKITPTVDQYLSNGYAAGAAARYKRALSDGKRPFTLQMGQLLYHSGKL
                     STAAPGLIKIGPNTGRLRMNPADMERLGLQDGASVKLTSDRGTLQMGVQFDQSVAPHT
                     CFFPEHFNEPPVKDLMSVHVDPVTGVPSFKRTGVTIEKV"
     gene            complement(10695..11939)
                     /gene="nuoD"
                     /locus_tag="VFH05_02680"
     CDS             complement(10695..11939)
                     /gene="nuoD"
                     /locus_tag="VFH05_02680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HET6677150.1"
                     /translation="MAFEDQRTTVYKVDPAHPESETLPTLRTEELLLNMGPQHPSTHG
                     VLKVILELEGERLVKSTPVMGFLHRGVEKLAEEGTYHQFIPHTDRLDYVCAMYNNFAY
                     CRAVEKLMNITVPDRAEYLRTIVAEVQRIIGHQFWLGTQALDIGAMTVFFYCFRDREI
                     LLDWFDELCGARLTTSWYRIGGVERDFTPSLFAKLKQFLEYFPSKIDEYVVFLEKNRI
                     WLARTKGVAVISAEDAVNFGLSGPTLRGSGVDYDLRKAEPYSAYPKCEFSVPLGKNGD
                     TYDRYWIRVMELYESVKIVKQCLEQIQDGPIMADVPSVTLPPKERVFTNLESMIQQFK
                     LFSQGFDAPPGEIYCGTEAHKGELGFYIVSTGGGKPYRLKIRAPSFIHMGAFDHMSRG
                     YMIADAITLFGTYDIVMGECDR"
     gene            complement(11950..12453)
                     /locus_tag="VFH05_02685"
     CDS             complement(11950..12453)
                     /locus_tag="VFH05_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET6677151.1"
                     /translation="MSLQQLSERVREHFAAGFVKAVEWRGDLAITVTRDTLHEVAQFL
                     RDDPAMDFDYIVHVSSVDWPDDEERFEVVYEFYSIRKRHRIRLKTRVPESDCVVDSLT
                     DLWKGADFMEREVYDMMGIRFRRHPDLRRILMPDDYTEGYPLRKDFPLRGKGWRDTFE
                     FLDETAR"
     gene            complement(12459..12986)
                     /locus_tag="VFH05_02690"
     CDS             complement(12459..12986)
                     /locus_tag="VFH05_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET6677152.1"
                     /translation="MGLIQLGRHDKDGMPDVITTSIEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQERIMQKR
                     VFVKQPEQVKASLKV"
     gene            complement(12977..13369)
                     /gene="ndhC"
                     /locus_tag="VFH05_02695"
     CDS             complement(12977..13369)
                     /gene="ndhC"
                     /locus_tag="VFH05_02695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET6677153.1"
                     /translation="MSGLELLLEYLTKYFPILLFIFIALAFGVVTLLLSYLVQPKYPE
                     PEKLSAYECGSEPFSDARMPFPVRYYIFAMLFVIFDIEVIFLYPWAVVFNDINKVSVI
                     GLVEMLIFIALFLVAYVYAWRKGALEWD"
     gene            13820..15316
                     /locus_tag="VFH05_02700"
     CDS             13820..15316
                     /locus_tag="VFH05_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6677154.1"
                     /translation="MTTLPSRTFLSCSLLAVVSLTGLPHAGADDLDTIRYGEAARTYA
                     AGSIQQSTPQDGVVNVITGDNQTVGNRMRLGQNDVLYLKLKHPGEAAVGDLYTVYKRT
                     RKVFHPATGQYLGYLITRLAIVQVVQVDKELTTVRAVRAFGAAAPGDPVMRFALPHTD
                     EAAVIPASSDVTGMVVELQADLQMTLVAQRNIVYLDRGSDDGLKPGDRMDLIRYGGAL
                     PPRQVGEVKVLSTEQHSSTALVTKSVSRILKGDQFQLKYQSDLIPPATPTKRSMDAPP
                     AGHSGDSIPVQNAAGETRYSFTDLMKQLRYESGEARITPEGYVTLSRLVELVKAAPAD
                     QLIRIEGHADNMEIGPSLKSVYPTNWDLSKARATGVLRYLVEKGGIDSARVSSVGYGD
                     SKPIVSNATEAGRSKNRRVDIVLYSPDSTSHTRSEPTNATDDGYRVSGLGADEQKPAV
                     SAVDHASSTEAAASEQPVSVPAQATPDLPGGEGHAVPAPIDSPVPVQP"
     gene            15417..17738
                     /gene="priA"
                     /locus_tag="VFH05_02705"
     CDS             15417..17738
                     /gene="priA"
                     /locus_tag="VFH05_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET6677155.1"
                     /translation="MTGDARLSTPIETETLYADVIVPRHLTRPFTYMVPAHLQSVLRI
                     GHVVSVPFGRSLIHGAVIAVTGNPPAGLDRQRLKSICALITAGRASEISPSLLELARW
                     VAETYVAPYGQCLRLVLPPRAKTPDRSRILLTRKGEEALATGGLPTQTELELLKRLKR
                     RPLGIPLSRLRVGKTRCPEDVLAAILTREWVHKVPGRPASHAVPPQPLMTSGSPDLWH
                     DQHKEASQAMEPSLPEWHARVDEALGRREAARLLVEAAPGVRLALLRHAVDRTVALGR
                     RVLVITGETTRAESLALALHDPTRVTTCLHSTIPDEKKADIWNLIREDKVSVVVGTRS
                     AVFLPLPSIGLIWIDREEDPALKEPMEPRYHARDVAWMRAREEQALLVFSSAHLSLEA
                     TVQAPEHVLRASVPREATPYIEVVDLRRHDRTVLLSPTLIQTMREAMVRQAGVLLFLN
                     RKAYAGALVCRDCGQVPRCRSCTVALAYSRQKRSVFCHYCGAADAIPDLCAACGGPRL
                     QPIGEGTERVEEEVKRQFPLARILRVDGETMRRAKDAAAMWNRIHRREWDVLIGTQLL
                     LRDDVVPRVGLLGVVQADAGLSVPDFRAAERTYHFLLDASRLVEPRGTGGRLIIQSYL
                     PTHHVIEAVVQQEEGRFRTEELAHREALGFPPLLRLIALHISGPAERSVAQAAQAWAT
                     RLRSLAAVARPGSQDGAAACAIGQTNGLTVLGPVLSPVARVRGRYRRQILVKSADAAS
                     AVEVVRSTLADLETTYPARQVKFDVDVDPIEMW"
     gene            complement(17743..18159)
                     /locus_tag="VFH05_02710"
     CDS             complement(17743..18159)
                     /locus_tag="VFH05_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677156.1"
                     /translation="MASSWTLRINAALGSLVVAMGAWLAWEGLAGVGGLMILVIAGCF
                     LVWRGRTLRLIWAWTTLFLGIECLAWPIITMLRIRSATTQPSDEQMGTILSAVLMGLF
                     SAVFWIAFSYGLFKGTDPNRLPQSPETQSPRRQRKR"
     gene            complement(18229..18924)
                     /locus_tag="VFH05_02715"
     CDS             complement(18229..18924)
                     /locus_tag="VFH05_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677157.1"
                     /translation="MTVASVTSPRRRGVPSPKASSIRLLLVDDHEVVRVGLRTVLHNN
                     QGITIVGEADSKAHAAIEAKRLRPDVILMDVRLPDGSGVEACREILSEQPNSRVIFLT
                     SYTDDDSVLAAVLAGAYGYVLKEIDSAALIRAIRTVSAGRSILDPNVTKRALEWIKTV
                     PAAEGPVRPSSLSPQEERVLALVADGLTNKEIATSLKLSDKTVKNYLANMFQKLHISR
                     RAQAAAFFVKRTV"
     gene            complement(18945..19028)
                     /locus_tag="VFH05_02720"
     tRNA            complement(18945..19028)
                     /locus_tag="VFH05_02720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18993..18995),aa:Leu,seq:gag)
     gene            19274..19984
                     /locus_tag="VFH05_02725"
     CDS             19274..19984
                     /locus_tag="VFH05_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET6677158.1"
                     /translation="MQGYHDLHPSLVRAATTASPASLAALFDVDNTLVPGEACEVRFF
                     RYLWRRGLVGWRELRRSVSWLVTQGRSVSIHPLRERKIYLEGKNSALIESCARDFFQR
                     EMVRNISPKGRDRLEAHRQSGHQLILVSGAPDFLITPLAETLNVSTLFAARIECQGAC
                     YTGAVIPPLPYGIGKRQLIVSHAQEKGLDLTSSFAYGDSPGDTEILDLVGYPVVVNPI
                     RGMLRIARDRGWPVVDWT"
     gene            19987..20622
                     /gene="queE"
                     /locus_tag="VFH05_02730"
     CDS             19987..20622
                     /gene="queE"
                     /locus_tag="VFH05_02730"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HET6677159.1"
                     /translation="MRVTEIFHSIQGESSYVGQPCVFVRLTGCPLRCTWCDTEYAFYG
                     GKEQSLEEILSQVEAYGCELVEVTGGEPLAQPDAIPLISTLCERGHRVLVETSGAIDI
                     APVDSRAHVILDVKCPGSGMTGRMHWPNLDRLTTKDEAKFVLADRTDYEWARDMLVQH
                     ALPGRCTVLLSPVFGALDPRQLAEWVLADRLPVRLQIQLHKLIWTPDMRGV"
     gene            20689..22200
                     /locus_tag="VFH05_02735"
     CDS             20689..22200
                     /locus_tag="VFH05_02735"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET6677160.1"
                     /translation="MNIMQIDAKVGRAETEAAEMLVLAHCEGERLNKQDTSALDKALG
                     GALSELLQSKEFEGRAGEILVYHTQGKVPSKRLLFVGLGKKNNLTLDAIRQAMGHAVK
                     RVRQTKAGAFAALVPAWTPRGHSALEVAQAMTEGAILGHYQFTAYRSDNGTPTEVGRM
                     TLLAPQKAQLRQLGEGIRRGVATAEATVLVRDLCNHPSNVMTPSRIANEAKKVAKEEA
                     LSLKILEQRDMEQLGMGALLGVARGSHEPPKFIVLEYNGSKKKNERPVVFIGKTITFD
                     TGGISLKPAENMEHMKADMTGGAEVLAAVRAAARLRLPLRLVSILPVAENMPGGRAMK
                     PGDIVKTLSGKTVEVQNTDAEGRLILADALAYAQRYNPAAMVDIATLTGACVVALGQF
                     AIGLFGTDEKLKAQVKKSGDKAGERVWEMPLWDEYFEQLRSDVADMRNIGGRGGGMIT
                     AALFLSKFAGDGPWAHIDIASTDWSERERAYIPKGPTGIGTRLLIQYLIDRSL"
     gene            22203..23330
                     /gene="nagZ"
                     /locus_tag="VFH05_02740"
     CDS             22203..23330
                     /gene="nagZ"
                     /locus_tag="VFH05_02740"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET6677161.1"
                     /translation="MARLELTSREKIGQLFMIGFMGTTVTPELSAFIKDYKPGGVILF
                     SRNLESVEQIVQLTNDLQQCSPKSPLLISIDQEGGRVSRLPQGFTIFPPCELIGRCNS
                     SELAYAAAATIAKELKAVGINMNMAPVLDVNSNPDNPVIGDRAFSSSAHAVGEMGLIT
                     AAGLQDQDVVACGKHFPGHGDTNADSHKELPVVEASRERLEAIEFPPFRRAAAAEIAT
                     MMTAHVLYKALDEALPATLSPAIISGLLREQMGYDGVVLTDDLEMHAIVDHYGPGDAA
                     VRALLAGCDVLLICKERDREIAAFEAVEKAVASGTVTMARLDQSVARIQRVKQRYLTP
                     YRPAMISEAKLIAGCRTHQALLQSLRQASHRMEKRSIASSL"
     gene            23356..23742
                     /locus_tag="VFH05_02745"
     CDS             23356..23742
                     /locus_tag="VFH05_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677162.1"
                     /translation="MGVKKGDLIDERKRHPDSCGLVVKVMGGGEGFEKIVCCDHELTE
                     LDVVPAMASAKGRKRGQLATGVVLDEKKLYPDSCGLRVMIMDGGAGFKEIRCCGHSLT
                     VSSKRELRYDLIRGSDPPQNGPEGNA"
     gene            23785..24240
                     /locus_tag="VFH05_02750"
     CDS             23785..24240
                     /locus_tag="VFH05_02750"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HET6677163.1"
                     /translation="MKRWCGVMEWLDRLGYLTAGFSLLVLGMLIFAHAWYVFMTKAGS
                     SGLLPSGLKLLNDLLLVIILLELFRTVIRFLQTEVLDLEPYLAVGIIACTRRVLTASA
                     ELSHLPNMTETQFYQYLMDVGLNVAVIMVLVVGVFLVRKRPAQALPVSP"
     gene            24301..24753
                     /locus_tag="VFH05_02755"
     CDS             24301..24753
                     /locus_tag="VFH05_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677164.1"
                     /translation="MPPISAYGIMPLPGMANLLEYVGSVHIYLGPYRGNPIALYLKRS
                     DAGCQIGPKVYPWNDVVGMGDSPNKAAADFEEKWKAKGLSADMYSGPSWEGGIKPERP
                     APPKPPATPKPAAQANSAPASQASAAPAASTPARVPSTPSGQSSGESS"
     gene            complement(24823..26322)
                     /locus_tag="VFH05_02760"
     CDS             complement(24823..26322)
                     /locus_tag="VFH05_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6677165.1"
                     /translation="MEREDVLSRPSLARPAAGRRPVISQLFRTKSIDQILADADHPTH
                     RLKKSLTAWDLTCLGIGAIIGTGIFVLIGTAIVGDAHRPGAGPGIVLSFVLSGLTCAL
                     AALCYAEMSAMIPVAGSAYTFSYATLGEMLAWLTGWNLILEYGVACVAVAIGWSGYFN
                     NLLQLAGLDLPQWATRPPGGTDGGIANLPAAIIVLLVTIILVVGIKESARATGIVVLI
                     KLAVILFFLGIGSTSVNPQNWTPFMPQGMAGVGAAAAIVFFAYIGFDAVSTAAEEARN
                     PARDVPIGIIGSLSLCTFLYIAVAAVLTGLIPSSQIDIHAPVAEALRLAGFKWGAAVI
                     AIGAVAGITSVLVVMMLGQIRVFFAMSRDRLLGPWLAAVHPRFGTPHRATILTGVAVA
                     ILAALIPIGEAADMTNIGTFFAFVLVCISVIVLRYTKPDQPRPFRLPFMPLVPILGTM
                     ACLGLMWQLPQLTWIRFGVWTVIGIVVYLSYGLNHSRLSAKALKQTSAS"
     gene            complement(26378..27118)
                     /locus_tag="VFH05_02765"
     CDS             complement(26378..27118)
                     /locus_tag="VFH05_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET6677166.1"
                     /translation="MKPLIGVTPDFNAGDRKEWGGREPTYFLRARYVRAIEDLGGIPV
                     VLPLVADLGARRRLLTHIDGLLLTGSGPDLAPELYGERQRYKFRVMSQRRTTFELEMA
                     HLARAANMPVLAICGGMQALNVAFGGSLYQDIAAQRSKPLQHRQPTPATRLSHDVTIT
                     SGSLLRRIVKTGTMRVNSSHHQSVKDVAPSLMASAVAPDGIIEAIESPSQRFFLGIQW
                     HPEFLFDQHKLHRRLFEAFLHAARKGTS"
     gene            complement(27122..27553)
                     /locus_tag="VFH05_02770"
     CDS             complement(27122..27553)
                     /locus_tag="VFH05_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677167.1"
                     /translation="MKLYVCAYTAATFRLVAVVLVLALGMVSVAPSAWSQESLPPSSE
                     TTEGGSASSAGMGAAAGVSTLLYLPLKAAFAIGGGIVGGLAYAFSGGNENAAKSIWTT
                     SLYGTYIITPDHLRGDRPIRFLGVPETEASAQAAAPMEPIR"
     gene            complement(27697..28149)
                     /locus_tag="VFH05_02775"
     CDS             complement(27697..28149)
                     /locus_tag="VFH05_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677168.1"
                     /translation="MSIDSEASQFRITLFIGPQPVKGKPFTYSTVYNVKKRSWKGGVQ
                     VAVELEESQINSLSSSIGFPEWLDQALVQVPVEERPAYQERAQDLFVQALAWCKLDLL
                     LQSGITQNNQCITADTFVTELNMVAINKRHFVASHVATELDLLPHHPA"
     gene            28165..29427
                     /locus_tag="VFH05_02780"
     CDS             28165..29427
                     /locus_tag="VFH05_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET6677169.1"
                     /translation="MADGHPSSLTLQSHNDKMPTMPDLAGEQRILEIQRVLREHDSTD
                     GWLFYDFRGSDLLAYRVLLLDPCMHVTRRWYYWVPAHGTPQKLVHGIEPHVLGSLPGD
                     TRAYVSWDQQRQELGRILTGNRRIAMQYSPMNAVPYVSRVDAGTMELVRSFGVDVVSS
                     ADFIQLFEARWTDRQLESHQYAAAALRRIVDDAFGHVSQCLTHGTRLTEYGLQQFILS
                     RIKDAGMVTSSPPIAAVNEHSADPHYGPTAIRSSTIQRDNLVLIDLWAKQADAGSVYA
                     DITWTAYAGSTVPEKHRAVFDVVKRGRDTALEFVRENLRAGYRPFGHEVDAACRRVIQ
                     AAGYGDQFIHRTGHSIGEEVHGNGANIDGLETHDTRRLMPRTCFSIEPGVYLAGEFGI
                     RSELDVYLTEHDALVFGLPLQTALSPLL"
     gene            29528..29665
                     /gene="tatA"
                     /locus_tag="VFH05_02785"
                     /pseudo
     CDS             29528..29665
                     /gene="tatA"
                     /locus_tag="VFH05_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            30359..31138
                     /locus_tag="VFH05_02790"
     CDS             30359..31138
                     /locus_tag="VFH05_02790"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6677170.1"
                     /translation="MDEWSSSRRSPLSLFLLFCLTFSGCLQEAPWPSPDQSKALLTVL
                     LKDHNPEVRRTAVESLGKIGDPTAVPSVVPLLTDPIPLVRGAAAQALGRLATPADEAA
                     VAALTQALADPADNVRQAAAMAIGDIEPSPRQLAVITTLAQAADVRVRRTVVHALQSL
                     DTSHVASALLPLVGDSDAEVRQGAVACFGNSDDVRAGRVLKQRLAEDPSAAVRAQAAY
                     HLGEVRGIDARSVLQAAAATEQDSGVRRWIEAELRSLRGSD"
     gene            complement(31113..31631)
                     /locus_tag="VFH05_02795"
     CDS             complement(31113..31631)
                     /locus_tag="VFH05_02795"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6677171.1"
                     /translation="MKFPVTVTPGHQGESLDIDPSREIISLHGANGALLGAVSWHDVI
                     ERISAGDEDIRFAHARAHARAPLAVKVKYTTPEGKQFDSLTGGVGGGGLFIESSQPLA
                     PGTELSIEFALPDRPWEKHKAKAKVAWTRNKPERFLLFPGMGVQFTDIEEKSRHDLVE
                     LVAALNRSRATT"
     gene            complement(31683..32204)
                     /locus_tag="VFH05_02800"
     CDS             complement(31683..32204)
                     /locus_tag="VFH05_02800"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET6677172.1"
                     /translation="MKYPVVSSLRHKGKTIELDSAREIITLYGINGDALGNLSWDFVI
                     DQILAYRKPPASREARTEPRVTLAFRVKFKTPEGQQFESRAGGIGGGGLFIESQSPLP
                     VGTKLSLEFSLPERGNEWLAAKGVVAWVCPKADQYTFSPGMGVRFTDIAADIRNRVLE
                     LVKSLQEVGRPAA"
     gene            complement(32430..33686)
                     /gene="clpX"
                     /locus_tag="VFH05_02805"
     CDS             complement(32430..33686)
                     /gene="clpX"
                     /locus_tag="VFH05_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET6677173.1"
                     /translation="MAKQEKMDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPSEIKHHLDQYVVGQERAKKILSVAVHNHYKRISAKEKD
                     VDDVELQKGNILMVGPTGTGKTLLAQTLAKYLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQASDYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTSNILFVCGGAFVGLEQIIEQRLNRKAMGFGAEIRGRAE
                     VRLGELFERVQPEDFLKYGLIPEFIGRLPVVATLDELDERALIKILTEPRNALTKQYE
                     KLLSFEKVKLKFTEGALGAVARKAFTQKTGARGLRAILEEVMLDVMYDAPSQKQIKEV
                     FITEDAILGKHPPIRIFEQDKDVRTA"
     gene            complement(33700..34329)
                     /gene="clpP"
                     /locus_tag="VFH05_02810"
     CDS             complement(33700..34329)
                     /gene="clpP"
                     /locus_tag="VFH05_02810"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET6677174.1"
                     /translation="MLVPIVIEQTNRGERAYDIYSRLLKDRIIFLGAPIDDIFANLII
                     AQLLFLEAEDPEKDINLYVNSPGGSVTAGLGIYDTMQYVKPPINTICLGQAASMGALL
                     LTAGTKGKRFALPNARVMIHQPLGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     IEKIALDTERDYFMSGEEAKRYGLIDEVITRAPKALKAVASEKDGAKDK"
     gene            complement(34373..35686)
                     /gene="tig"
                     /locus_tag="VFH05_02815"
     CDS             complement(34373..35686)
                     /gene="tig"
                     /locus_tag="VFH05_02815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET6677175.1"
                     /translation="MKMEVTELGPMKRALKIEVPVEEVAQRFVLAYKELNRQVRVPGF
                     RPGKAPQALLEQRYAKAVEEDVVRSLVPDFYDRAVRQAGISPVVVEIPPLERVKIKRD
                     APFTFTATVEIKPKIELRDYKAPNPISLKPDTRTVTDEQLAKGMNVLREQHARLEAPP
                     AGHRVVEGDFVILTLEGFLDNEPLEGSKKAGHLHKVGSNATVLGLEIDRHVLGKAEGD
                     TVEISQPYPASHPDSRVAGKTVTFTVNINAVKIKTLPDLDDEFAKDCGAYASLAELQE
                     RLRTQMEKALKREVEDTYKDTIVKRLVETHHFDLPETLVERELEAIIRQHMQRHRRAG
                     ADAPSALETEDLTKLRQENRDEAARRVKVGLILEAIAEKEQLTVTQEDLNNEVQRLAS
                     ELKIAHSELVKMIQAGGQDSIEELRARILAEKSLDFVYRNAVIQG"
     gene            complement(35733..35815)
                     /locus_tag="VFH05_02820"
     tRNA            complement(35733..35815)
                     /locus_tag="VFH05_02820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35779..35781),aa:Leu,seq:tag)
     gene            36094..37176
                     /locus_tag="VFH05_02825"
     CDS             36094..37176
                     /locus_tag="VFH05_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6677176.1"
                     /translation="MRIGLLLISLTLLALTIDSSPVLAYRDYLTAEQKAQLEKIHTVL
                     VEAIALTDKGTVDAGPITEVAARRLGEMGYAVVRDAGKPHDATFKVKCEQRKTWEGTT
                     AAGGDADLPDAPSRLWKGPACQLSYLLGETKIKWQKEVRTEFEDAAAAAQSANAGDPG
                     AFAMSQLRAKLEQYDFPVLLAAEWGHPDRLLKLLDASDTPQVRKIKIISLLGEMQADE
                     ALPQLKAALKDKNLSKEAAVALGSMGKEGIPILVDILKHSKQPELQAAAAKGLGDLGN
                     VHNDSSVVPPLLEMLDAPGVDITVQTEVAWALGRVPDRRSVKPLFDLDKKLQKIRKDP
                     PDPQIKKLKEAVFWSIKQVYTEDQYS"
     gene            37356..38600
                     /locus_tag="VFH05_02830"
     CDS             37356..38600
                     /locus_tag="VFH05_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6677177.1"
                     /translation="MNDMSRRRFLQWTAATGGTLALGGLMNRGALLEFGVRIAHADEP
                     IKIGILDPLSSPYKTSSIHDVHGANVAVDVFNKKGGVLGRPVAILEADDASNPDQAVK
                     VATKFIKDDRVDVLMGTFNGDCALAVSALAAKENKLFMVTAAHIPELTGAACNSHTFV
                     FMPHARMLAHAVAPHLAKAYGTRWFMITASTIDGKSCAQALVDASQAHKVEIVGETVT
                     PFGTTDFGPAFTAAKEKQPTAIILNLYGWDLVNALKSYAKLELAKEKIGIGGLISGEQ
                     IGRPLGYAGNAGIWGLIWDPKINTESSKRFIQGVIDKYNHTPTSRCYLGYAAMTQILE
                     AIQRAGSTDTQALIKTLEGHEFDGLKEGKSVFRASDHQHVQDVLVGEAYGKELGLGHY
                     KILSTISGPANPGAADQQLCKH"
     gene            complement(38654..39010)
                     /locus_tag="VFH05_02835"
     CDS             complement(38654..39010)
                     /locus_tag="VFH05_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6677178.1"
                     /translation="MDAPVFLLSLFGSGEHQLKSAGSDALKHVGAILKDFPEYKVSVD
                     GHTDNRPIRNELRKKFPSNKELSEARATNAAMALGEGGLTAVTTHGYADTRPIVPNTT
                     EPGRAKNRRVEVRVTR"
     gene            complement(39015..39443)
                     /gene="mscL"
                     /locus_tag="VFH05_02840"
     CDS             complement(39015..39443)
                     /gene="mscL"
                     /locus_tag="VFH05_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET6677179.1"
                     /translation="MLKEFKEFAMKGNVLDMAIGVIIGAAFGKIVTSLVSDVLMPPIG
                     LLMGKVDFSSLFLNLSSTPQPSLAAAKAAGTPTINYGVFLQTVFDFIIIAFVIFMLVK
                     QINRLKKPTAPAAATTKDCPLCLSTIPLKATKCAHCTSSV"
     gene            complement(39482..40582)
                     /locus_tag="VFH05_02845"
     CDS             complement(39482..40582)
                     /locus_tag="VFH05_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HET6677180.1"
                     /translation="MGSSVLVGIVLLLILIGGPTTQAAAEEPAPPAPAPVDVVTLKDG
                     SVIYGEVLEMAGGILFLKNPSVSDNLIKLKWEDVSKLNITHPIPFHLKEGTILNGTAT
                     PGPDGVLVIHAEPLKGTMDIPMSDVSSLNPLIQPPVIYIGNLTGGYSQTTGNSHLRNA
                     SLLGELVARSELLRLTMNVRYVYGDDSGAIITRNARATIKLDFFITKRFFWFASAYFE
                     NDQFQDLKLRTALASGPGYQWIDREDFSGIGKDMTLYTEAGLAYFNEDFNVAGDKSSV
                     RARISVKWNWPFFEDRFTLYHLSELFPSLQNTSDVFLTMDNGIRLKIWQGFISSFQIT
                     TRYNSRPAPGTGDTDNLYLITLGYALDTTRKR"
     gene            complement(40867..42744)
                     /locus_tag="VFH05_02850"
     CDS             complement(40867..42744)
                     /locus_tag="VFH05_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET6677181.1"
                     /translation="MKILSVIVIVLALLIVALLSLPFLVDLAAYQDQYKPLIEEALNR
                     KIHVQGIRLTIWPRIGARITGFTVMDDPSFTANPFASLSSLDVGVKLWPLLTKRIEVE
                     EITLRDPVITVIKNKAGVTNVSTIGPKTPVPADRRQPESPQQQGDPLEALALLAVDRL
                     SIDGGTVVYRDLSTAPETEYRIQDFVLALKSVHLGETPTIHFSATTLPHNLPVTLDGS
                     FGPLVQALEIKQYQFTLAVGRMVVALHGALLGGQLNAVVTAPSISTADVPVALPLTRP
                     VEIKDLRLVAKAPYPLKSGASPLALADVTDLTLAVVMGKSSLAVRGNAIGGHARVMVS
                     SPSVDTADLPVESGLKKPVELRNVELNADLKGQEVRLSNFSLEVFNGQVKAQGGTSLG
                     TSTPPFNGKLTLQGVQIGQALAAVDPQSPLQVSGTAAADVAVAGRGFSWDDLMRALEG
                     PGHVEIKDGKIEGVNLMAEAVALLKVAGISLEQAKATAFSTIESDFTIKHGSVNAQKI
                     LMDSHDFQATGHGTVGFDQTLNLAMNLNLSQTLTQKIAGSALVKAASQGGRLRLPLLI
                     TGTLQKPSFALDARGLTGKVQEQVQEKVKETVEGLMQGTTKPADLKKEGKELLKGLFG
                     R"
     gene            42855..42944
                     /locus_tag="VFH05_02855"
     tRNA            42855..42944
                     /locus_tag="VFH05_02855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42889..42891,aa:Ser,seq:gga)
     gene            43376..43475
                     /gene="ffs"
                     /locus_tag="VFH05_02860"
     ncRNA           43376..43475
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFH05_02860"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            43615..45429
                     /gene="dnaX"
                     /locus_tag="VFH05_02865"
     CDS             43615..45429
                     /gene="dnaX"
                     /locus_tag="VFH05_02865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET6677182.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQPHVVQTLVNAVTSKRIAQAYLFS
                     GTRGVGKTTVARILAKALNCEQGPTGTPCTTCVNCTEIAQGHSVDVIEIDGASNTSVD
                     DVREIRENVKFAPFHGTYRVYIIDEVHMLSNSAFNALLKTLEEPPPHVVFIFATTEIH
                     KIPATILSRCQHYNFRRIAKTEIIERLRHVAGQDGIALEDRSLAALARASEGSMRDGL
                     SLLDQAVAFGGKSIVHTDLEALLGAIPQELVRSLIRAITSQDSAGALRTLSQLVEGGH
                     ELRAYCADVVEYVRHLLVVSVTPPQEWPALIEVSSDDLAQLATDKASLTPEQIQQIFT
                     LFLEAEDALRFSAHPRFVLETAAIKATRLLGPQTTISDQPGRAVSASGAPHPSAPRSD
                     KGSSAPIGGSSQPARAPQPQRAVTGTSSSSSSPGSGVPAATSMPRADKTPATQPMKQV
                     GQSSSSAAPARPPQAIMLNWEQFQEEVGHAFPNIAPFLEMGRLVGIEGNVVTIGFAKQ
                     ATVARGMLEKADNIQALASLCERMGGQPVRLRVIELTEADAPGPTMAEIRAAKEQEQK
                     VVLFERAKAHPLVKQALEMFGAELADVRTIANQQEVQE"
     gene            45426..45749
                     /locus_tag="VFH05_02870"
     CDS             45426..45749
                     /locus_tag="VFH05_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET6677183.1"
                     /translation="MKNPLGNMANLMKQAQQMQAQMAKLQEEAATKTVTGSAGGGMVT
                     VTANGALEVVSVRIDPEALKDADAEMLQDVVLAATNEALKNARQLMAEQMKSVTGGMN
                     IPGLF"
     gene            45760..46362
                     /gene="recR"
                     /locus_tag="VFH05_02875"
     CDS             45760..46362
                     /gene="recR"
                     /locus_tag="VFH05_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET6677184.1"
                     /translation="MAVDQQGLLSRLVRELVRLPGIGQKTAQRLAFHILKADRDDALR
                     LADAIRAVKDGLSFCRQCRNIAEGELCEYCVDPKRDQTRILVVEEPSTLYAVERAGAY
                     RGLYHVLLGALSPLDGVGPSDIRAEELVDRVKLGGIQEVILATNPTIEGEATAIYLTN
                     QLKPLGTRVSRIAYGIPVGMDIEYADEVTLLKSIEGRRDL"
     gene            46494..46913
                     /locus_tag="VFH05_02880"
     CDS             46494..46913
                     /locus_tag="VFH05_02880"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET6677185.1"
                     /translation="MKTKASTRRNPVAVKRGSKAASAGGKYGAIRRELQKQRQAILDE
                     TGEGLTKREGPEAFPDVSDQASAEVDQNFSMRIKEREQKLLKKIDEALDRLSKNIYGI
                     CERCEEEIPYQRLKARPVTTLCIACKTLQEQEEKIRR"
     gene            complement(46938..48062)
                     /locus_tag="VFH05_02885"
     CDS             complement(46938..48062)
                     /locus_tag="VFH05_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677186.1"
                     /translation="MGLVVCLTVSLAAACETPNPRPNLPPSESDLVLLKPGTLCQRKT
                     DFLQQHPQSSLTIKPWGTGQELMLPASRSASHGDESFFFDDDGTLVGALFTFSSGLDL
                     DPYSVLRHTLTLLKPTLEFYLSVANFAGKSAMDSSSLYETGDEKTTTQYLVISARDRP
                     TLLQASVTIDPYVRLFSPYRREFLNRLRQPSGVQPGQQVDSQGAEDKEPFASLQQFAR
                     GQTAQLSYCGTQSYDIAADAYRKSIASGFTNKVWLAEAHHKFGQSLFGLGQYEKAKAE
                     MLQSLAIRPNTPETLNNLGSTQLKLGDKAGALASFEKAVTLRPNYAIARFNLAEAVEP
                     TNPKRALAEYETYLALVEGVPDEADRIALVQQRVKKLKPQ"
     gene            complement(48221..48568)
                     /locus_tag="VFH05_02890"
     CDS             complement(48221..48568)
                     /locus_tag="VFH05_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677187.1"
                     /translation="MKAKLIAVSLLLIAGLIGCAGHQQAADGDIGYVAPASIYSVMDS
                     TALVYTDVAAGSPLNDNPWRWLGFLLHPIGQGLDYGVNRPIYSLTSGMPYLFGYTSED
                     AMLDSQRRRASQY"
     gene            complement(48721..48795)
                     /locus_tag="VFH05_02895"
     tRNA            complement(48721..48795)
                     /locus_tag="VFH05_02895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48761..48763),aa:Val,seq:cac)
     gene            48815..50245
                     /gene="trmFO"
                     /locus_tag="VFH05_02900"
     CDS             48815..50245
                     /gene="trmFO"
                     /locus_tag="VFH05_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET6677188.1"
                     /translation="MPFGWFNPSAGRFALTSPKYDSYNALLLDLSLAERSTQMREDIV
                     IIGGGLAGSEAAWQAAHRGAKVTLYEMRPKESTPAHKTGGLAELVCSNSLGSADPQNA
                     AGILKEEMRRHQSLIIKVADQVRVPAGSALAVDRDQFSFLITQALDSHPNVRILREEM
                     TEIPTDCVCIVATGPLTSDKLSQAIARLTHSNHLYFYDAISPIVDADSINMDIAFRAS
                     RYGKGGDDYLNCPMDEATYQAFYDALLAAEKVQPKAFEQTPYFEACLPIEVMAERGRQ
                     TMQFGPLKPVGLEDPKAGRRPYAVVQLRTENAHRSCYNMVGFQTRLTYGEQKRVFRMI
                     PGLAQAEFLRYGSLHRNTFINSPQLLHNTLQFKERPTLFFAGQLVGVEGYTDSAAMGG
                     LAGINAVRELDGLPPLTPPPTTAHGCLLSYITASDPRHFQPMNMNFGLFPPLTVPVKD
                     KERKRQLISQRALEDHAAWMSRSGLS"
     gene            50218..51156
                     /locus_tag="VFH05_02905"
     CDS             50218..51156
                     /locus_tag="VFH05_02905"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HET6677189.1"
                     /translation="MDESIRAFITFLESEQRASPETVRNYASDLRQLDRFLVSSRLVS
                     SPVDPASLSSDAVRAYLEWLDRKGEKHASLARKLAAIRSFYRYLSRRGLTNRNPVDAL
                     RTPKQARSLPRVLTKDDADALMAFPAGETAGSLRDRALLETLYSTGARVSELVSLNVG
                     DVHGSEGLLRLRGKGRKERIVPIGELALEAITRYRASLPTSLQRAGHTCPLFYNLRGG
                     RLTSRSVARIVARYSSRLSGGPVSPHTLRHSFATHLLDEGADLRAIQEMLGHASLSTT
                     QRYTHVATDQLLAVYDRAHPRSGRTSNGRTSKNGSS"
     gene            51247..52143
                     /gene="argB"
                     /locus_tag="VFH05_02910"
     CDS             51247..52143
                     /gene="argB"
                     /locus_tag="VFH05_02910"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET6677190.1"
                     /translation="MNKLIRKANVLIEALPYIRTFRGKTIVIKYGGNAMTESSLKERF
                     AQDIVLLKYVGINPVIIHGGGPQIDEMLDRLGIESKFLHGVRVTNEATMEIVEMVLAG
                     KINMEITDLINRHGGSAVGLSGKDGGLILSKPLTARAWAESLERDLEEEDAESDFGFV
                     GDIEKVDPSLLFKLQGDHYIPVIAPIGTDREGNTYNINADLVAGAVAGALRAEKLVMM
                     TDIKGIRDAKSRHLSTVSRKDVQRMVKKGTITEGMLPKVHACLDALEQGVGKAHIIDG
                     RMPHAILLEVFTRKGIGTEITA"
     gene            52194..53039
                     /locus_tag="VFH05_02915"
     CDS             52194..53039
                     /locus_tag="VFH05_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6677191.1"
                     /translation="MKALVGERHPHSAPEALARAAAYVAAQFAGFQWTTTRQSFRALG
                     KSYSNILAVKAPAGRPAKERQAPLLIGAHYDTVMASPGADDNASALAVLLEVADQLRY
                     TGVTRPVWLAAFCLEEQGLLGSRAFALHLKQAGQPLYGAIVLECVGYVSHEAGSQGTP
                     PGVPISVPTVGNFLGIIGNEASRHLIAAVERSARSAAPAMPTLALAVPGRGEALPDVR
                     RSDHAAFWDEGYPAVMLTDTANFRNPHYHLPSDTLDTLDLGFIEAVSRTVAASVVELA
                     GKATR"
     gene            53051..>53515
                     /locus_tag="VFH05_02920"
     CDS             53051..>53515
                     /locus_tag="VFH05_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677192.1"
                     /translation="MSAVSRRAIARVFDHLDYAALGKVYCYEGGREFWRAKREPCRRL
                     GSQIGTALLARLKPGGRSLYVGAGVAELPAMVIESLDLYRSVLPYNLRRREVALLNRA
                     CRALPLRFYAVDAARASGPFDHLWIVSVLNDPERFPQLAALSYGRAVPVTFDA"
BASE COUNT        11620 a        15258 c        15655 g        10982 t
ORIGIN      
        1 aacagaatcg catcattgcc catcttggtc ccggcggcca ggccgatgag gacattgccg
       61 atttgcgcga tgcctgagta ggccaacaat cgcttcatgt tcctttgcgc gatcgccacg
      121 atattgccgt aggtcatgga gaggatggat gccgcaacca acaagagaac ccacgtcggt
      181 ttgaatgtcc caagggcgac aaagaacatg cgcaacaaga tggcgaacgc cgcgcctttc
      241 ggggcgatgg acaagaaggc cgtgaccggt gtcggcgatc cgtgataggt gtcgggaatc
      301 caggaatgaa acgggacggc gccgatcttg aatcccagcg cgccgaaaat gagcaggaag
      361 ccgatgatga gtcccggcgt ggcctggacg cctttcatgt ccgagaagac cagcttgccg
      421 gtctctccat agaccaagct gatgccgtaa gccagcagac ctgccgcgaa tactcccaga
      481 atgaagaatt tcagtccggc ctcattgctg gccacatcct cgcgcaagta ggaaaccagg
      541 acataaaagc cgaacgtcga gaattcgagc gtgacaaaca cggagagcag atcattggca
      601 gaggccatga acatcatgcc caacgccgac atgaccacga ggaagtagta ttcaccgcgg
      661 aaaaaggtga accggtggat aaattcgatt gaagccaaga tgacgaggat cgtggcggcg
      721 acaatgaaga tcttgaaaaa cagcgccatt cgatcgacca cgaacatgtt ggcgaacagc
      781 gtgccggaaa tgccggagaa gtcgaaccat gccaggcagg cgagcgtccc caggagaccc
      841 gctatgctca gatacgccaa gtgctctttg gggagccgtg gaaaggaaaa gtcgacaatc
      901 aggaccagac acaaccagca agtcaggaat atctccggca gcaacaggag gaggtcggag
      961 ggggacatgg tcagcgtgaa ggtcatggca gtcctgtcac agtctgaggc ggggtctccc
     1021 caggtttggt ggcgacgagg ggacttggcg atatcgtcac ccggctcgtc ctctgtcgcg
     1081 ggacggcttg ctcgtcgctc gcctcagcga ccggcacgac caaggtgatc ttggcgacca
     1141 gcgggtcaac gccggaccgc acgacgttat acatgtgcat gggaaagaga ccgaagccga
     1201 tgctcaccac aatcatgatt aataacggga gccgatcgac cactgccacc gcatcgtgcg
     1261 agtggctgta tttctgatcc atcggcccgt agaagagccc gcgcatcatt ttgaacaaat
     1321 acgccaacgt aatgacgatg ccgatcaccg ccacgatgac ctggaacgga tacttatccc
     1381 aactgcccac gatgatcatg acttcggcga tgaaattgac ggtgcccggc atgccgactg
     1441 aagccataca ggcaatgacg aagcagccgg caatgaacgg catccggttc gagagaccgc
     1501 cgagcgatgg aatatcccgt gtatgggtct gatcatagac ccagcccgcc atggcgaaga
     1561 gcatccccgt ggccatcgcg tgtgcaaaca tgtaaatcac cgccccactc aagccgatgt
     1621 aattgagcgc cgccatgcca agaaagacat agcccatgtg acttgagctg gagtacccga
     1681 tgacgtactt cgtgtccttg gcgtaaaacg cgacgaaccc gccgtagata atgctgaaga
     1741 tgcacagcac ggcggcgatg ggcatcaatt cgcgtgtggt atcgggcaga atttcgaagg
     1801 ccacgcggat gatcgaaaaa tgccctagct tcatcaatac ccctgcatgc agcatactcg
     1861 tggcggccgg agcggccgcg tgtcccaccg gggaccacga gtgtagaggc cacagcggtg
     1921 cgatggaggc gaatccgaag aaaatgagga tccagatgat cttatccagc gtcgttccga
     1981 gcactgggat attcatcagc ttggcatgtt cccgcaactg caagatgtcg aaggtgttga
     2041 gacccgaata gcggtagatc agcaagatgc ccatcaaggc aaccacggcc caggcagaca
     2101 ggaaaagcac caacttcatg gccgcatatt ctttactgtt cgcgccgaaa ttaaagataa
     2161 agccgactga gtctcgctgt ttgagtccct cctgatcggt catctccaag tactttttgg
     2221 tatggctccc ccacatgccg agcaaaaggt acatcgggat gacggacatc tcgtagaaga
     2281 aatacagaaa gaagagatcc aacgacataa acacgccgat tgtcgcggcg gcgaggatca
     2341 gcagccagat atagaattcc ttcgtccggt ctttgatgtg ccacgacacg aaaatgccgg
     2401 cgaataatag aatggatgag gccagcacca ggggggtgcc gatgccgtcc acgcccaagt
     2461 gtagcgaaat gccgagctgc ctcgaccatt cgaatttttg gatgaactga aatccgcctc
     2521 tgaccggatc ataggcataa aagaggtaca gggaggcgag cagcgaaatt ccggccgaac
     2581 tcgcggcgat gcttctgacg agtacgggct ggcgattgga tgcgaagatg agcgcgatcg
     2641 cgccggcgaa tggagcgaac agaatgtaga gcagcaggta gtcgctcatg acctcaatgc
     2701 accttctctg aaaggggcac ggcgacatgg gctgcggcga ccgttccatg attcaaccgg
     2761 tccaccagtg cctgaacaga tccattgatc atgcgcagga acggcgctgg atagagcccg
     2821 atccaaagaa tcatggcgga cagggacagc gcgatgaccg tttcgcgggg atgcaggtcg
     2881 ccaatggatt ccctgacggc cttcccgacc ggcccgagca cggcccgttc gtagtaccag
     2941 aggagatagg ccgcgccaaa gatgacgcca gtgaccgcga tgccgccgta caaccaatgg
     3001 gcttgaaagg ttcccaacag gatcagaaat tcgccgacga acccgttggt gcctggcagg
     3061 ccgattgagg ccaacccgat caagaggaag aacgtggcga gtacgggcgt atgtttggcc
     3121 atgccgccga aggccaggag ctgtgtggtc tgctgtctgg tgtaaagaaa gccggcgatg
     3181 aaaaacagcc cggcggtggt gaagccgagg ttgatcatgg tcaacaagct gccttgaaga
     3241 ccctgatagt tcaacgcaaa caggccgacg acgataaatc cgaggtggct gatgctgctg
     3301 taagccagga gtcgccgaaa gtcgggctga atcaaggcga caattgcccc atatacaatg
     3361 gctgcaagcc cgagcgccat catcacggaa acgacggtcg ggtgcttcga ggcgtcaggc
     3421 aacagcggga tgctgaagcg catgaatccg aaggttccga gtttgagacc ggccagcacc
     3481 accgccatgc cgatgggccc ttcgagcaag gcgtcgggca gccaggtgtg gaacggaaac
     3541 accggcgctt tgaaggcgaa gcccaggaac atcagccaaa aaatcagaag ttgttggttc
     3601 agcggaacgg gaaccgccag cagttccaac agatcgaagg agtacaactg gtccatgtga
     3661 tgcagggtgg cgtagttgtg ataattgata tccaggagcg cgatgcccac cagcatgaac
     3721 acgctgccaa gcagcgtgta gagcacatat ttcaatgccg cgtaatgccg ttctgcgccg
     3781 cctccccaga gcttgatcag gaagtagctc gggatcagca tgagttccca gaacacgaag
     3841 aaaagaatca gatccagcga gacgaatacg cccatcgtcg tcgtctcgag agccaggagc
     3901 gccatcatat acagcttggt ttgatagcgg atcgtgtccc aggaatagat gaccacgagt
     3961 acggtcagga aggccgtcag cccgacgaac agcacgctaa tgccgtccac ggcgacgtgg
     4021 taactgattc ccagcgcggg aatccagggg acgtgctcca caaactgcat ggcgggagag
     4081 tccggcacga accgcatgag cacgaagatt gccaacgcca gttcaaccag cgtgatcgtc
     4141 aacgccgagg tgcgtataag atcttcatcg tcgaagagcc aaaggaccgc agcgccaacc
     4201 agagggaaaa agagcaggca cgtgataatc ggaaatgtcg aatccaattg ttccagcatg
     4261 atgtcgttcg tctagcgttg gacgatcagt tgaatgacga ccgcgagtaa aaacaacccg
     4321 gccacgatga tggccgcata atggtgcacc attccgctct gcaattgccg ccactgtttg
     4381 gccatgaggt ggttgccgta cccgacgacg ttgatcccgc cgtagatcac gtgcttctcc
     4441 acccaggtcg agccctcggc gcccagttcc gcaagccggg cgactgctcg gacgacgccg
     4501 tccaccacga cccgatcaaa ccagtcgagc atcagcccga acttcttggt cggctctaca
     4561 aatacgagat cgtaaaattc gtcgacgtag tacttattca acaacgtggt gtacaccgct
     4621 cccgccttgg ccgcccaccg ctcgggagcg ccaggattca tactgtacaa gtaatgggct
     4681 aagccccacc cgactacggc gataccgctg gcgatcatca tcagggacag gaccagccca
     4741 gtgctgactt catgttcgcc gctggcacct gcggctggcg ccaggaattg atgtaaccag
     4801 ccatgatcgg ggggaaaccc gaggaacccg ccagccaaag agaggagacc caacaccatc
     4861 aaggggccga tcatcaccat cggcgattcg tggacgtgtt cttcagtgtg atggtccatg
     4921 cgggagtgac cgtaaaacgt caagtaggtc agacggaaca tatagaagga ggtcaggagc
     4981 gcaccgatgg cggccatgcc atagagcaca tagtgatgat gcgtaaaggc atgggccagg
     5041 atttcgtctt tgctccaaaa accagcgaac gggggaatgc ccgcaatggc aatggtgccg
     5101 ataaaaaaca atctatgggt ccaggggatt ttctggctga gtccgcccat ctttcgaatg
     5161 tcctgctcgc cggataacgc atggatcacc gagccggcgg agaggaacaa cagcgccttg
     5221 aagaaggcat gcgtcatgag atggaaaatc gcggcagtgt aggcaccgat tccgcatcca
     5281 aggaacatat atcccagctg gctgacggtc gagtacgcca gcacccgttt gatgtcgttt
     5341 tgcacgaggc cgatcgtcgc ggcgaacagc gccgtcgcgc cgcccacgag tcccaccaca
     5401 cccatggcgg tcggggaaag atcgaagatg acgtggttgc gcacgatcat gtagacgccg
     5461 gccgtcacca ttgtcgcggc gtggatgagg gcgctcacgg gggtgggacc ctccatggca
     5521 tcgggcaacc aggtgtagag cgggatctgc gcggacttgc cgacggcccc aacaaggaga
     5581 cagagtgaaa tcgccgtggc catttccggg gacaagtggc cgacttgcgc aaagactttc
     5641 gtgtaatcga gcgtcttgaa attgatgaag accaggaaaa tcgcgagcag gaacccagca
     5701 tctccgatcc ggttgacgac gaacgccttc gaagcggcct tggccgccga gaccttgtca
     5761 tagtagtagc cgatcagcag atacgagcac aggccgacgc cttcccatcc gatgaagagc
     5821 acggcgtagt tgttgcccat gaccaggagc agcatcgaaa ccatgaacag gttcatgtac
     5881 gtaaagaagc gcgtgaatcc atcttctccg tgcatgtacc cgaccgaata cacatggatg
     5941 aggaatccca ctcccgtcac cacaagcagc atcacgcagg tcagcggatc gacaagaaag
     6001 gccatgttaa tggagagatt gcccccgaaa atccactggt aggccacgac ttcatgcggc
     6061 gtttcgctgc gcacgatgtc tgcaaacacc aggatcgtgc ttaggaatga caggccgact
     6121 gaaccccagg cgagacggtg agcccactca tgcgaccaga aacgacgacc gaacacgccg
     6181 ttggcgatga cggcgagcaa tggaaacact ggaatgagct tgatcagaag atcggtcatg
     6241 atggtccgat ctcgaaggcg cggacaatag cgccgatctc atcgcgtgtc ccgggaaggc
     6301 gattgcgaag caaccggtgg ttcgggtggg tagaagagca tcgctggggt gaggcggtcg
     6361 gatagatgag cagcatcgtg agtttgttta ccacttgagg agattcattt cgtcgacatt
     6421 tgtggagatt ttccctcgga agaccacaat gatgattgcc agtccgaccg cagcctcgcc
     6481 ggcggcgatg gcaatcacga acagcgccac caattggcct gccatagact ccaggtagtg
     6541 ggaaaatgcc acgagattaa tgttggccgc gttcagcatg atctccaccg ccatcaggac
     6601 gatgatgaaa tttcgtctga tgagcacgcc gagtaacccc gtaatgaaca ggacagcgct
     6661 gaccgcgaca taggcgctta atggcaccat agtgttctca accagaggcg cgtggaatca
     6721 gacgtgaggc atgcacggcc tatcgcgtca attcttgcct ctcatgtctt ccagaggttt
     6781 gggtgttttg gctagcacga ttgccccgat gatggcgccc aacaggaaaa tgccgacaat
     6841 ctcgaactgc agtaagtact cactgaacat cttcatgccg atcgcatagg tgtcgccctc
     6901 ttgcaggacc gcgcgggcgg gcgcatcgcc ttttgccccg ccatatggag acttgaacaa
     6961 taaggccagc acatacaatg aacccaggag tgtcggcacg acgaaataca ggtaggtgct
     7021 gtgaaaataa cgctcttcgg tcttgagatt gagcagcatc agcacgaaca ggtacaggac
     7081 gagaatggcg cccgcataca ccacgacttg gacggcccag aggaattcgg cattgaggag
     7141 gacgaatagg ccggatacgt gcagaaggag cgccagcaaa gccaatccgc agtgcactgg
     7201 gtttctcaag gcgaccgtga gcacggcggc agccatgctc atcaaggcaa aatacgcgaa
     7261 gaaaacggga atcatgatcc gcgtccgctc aactcaaatg tttcggcgcc ggttgcgtcg
     7321 acttaatgac cgactgcggg aagtagtaac ggtattcccg actttcctcg atgttctttt
     7381 cctgattgtg ggcatacagg tatttcttgg catcgtcgag gtgccgctcg ccgatgtcat
     7441 acaaccgctt cttgtcaaag agcaacgtcc gcttatcgtg cgtggaaaac tcgtacatct
     7501 tggtcatggc cagcgcgttc acgggacagg cttccacgca gtacccgcaa aacacgcact
     7561 tggtgatgtc gatgtaaaac tcggtggcat agcgctgaag cggccgttcg ggatcttcgg
     7621 cactgatgac cttgatgcag cgggatgggc aggcggcttc acacaggtca cagccgacgc
     7681 aacgttcctg cccgtcgtca tagcgcaata atcccagcgc gccgcggtgc gtgtccggaa
     7741 tagcacgctg ttctcgcggg tactggaacg tgatgggctt gtggaacata tgcttgaacg
     7801 tcaccttcat ggcgtcccag atctcgtaga agagggcagc ctgaaggaac ttctttgtcg
     7861 catttctgac ggtcatgggc gcgctacacc ttttcgatgg tcacaccggt ccgcttgaat
     7921 gacggcacgc cggtgaccgg atccacgtgg accgacatca gatctttgac gggcggttcg
     7981 ttgaagtgtt cgggaaagaa acaggtgtga ggcgccacgc tctggtcgaa ctgcactccc
     8041 atctggaggg taccgcgatc ggaggtgagc ttgaccgatg cgccatcttg cagcccgagg
     8101 cgttccatgt ccgcagggtt catgcgtagg cggcccgtgt tgggtccgat tttgatcagg
     8161 ccgggcgcgg ccgtggacag tttgccggag tgatacagca gttgtcccat ctgtaaggta
     8221 aaaggacgct ttccgtctga aagcgctctc ttataacgcg cagctgcccc ggctgcatag
     8281 ccgtttgaca aatattgatc gaccgtcggg gtaatctttt tcggctggcc caaattgtaa
     8341 taaccgggaa gcagtttcat gatctcagct tgcacgtcgt tcgccgagtc gtattcccac
     8401 tggcaaccta atccatttgc cagcgaggtc atgatctgcc agtcagggac gctttctcca
     8461 atggcatcca tcgcctgacg tacgcgtaag acccggcctt cgaggttcgt gaatgtgccg
     8521 tccttttctg ccgaagtggt cgccggcaat acggcatggg ccatgtttcc ggtttcagtg
     8581 aggaagggat cttggacgac cagcagctcc agacggtcca aggcggctcg aacttccacg
     8641 gaggccggga gcgtggccaa tgggttctct ccgaccacat agagcgctcg gatctctcca
     8701 ctgcggcagc gtgagaggat ctccatgaaa tgcgcgccgg ttccgattgc gggaagcgtc
     8761 gcatcccagg ccttcgccag cttatcacgc atggcctgat catcgaagga ggccaggccg
     8821 ggaaggaact ccggtgcaac acccatgtcg gcggcgcctt gttcgttcgc ttcttcggtc
     8881 acggtattga cgccgcaacc gggacgtccg agcttgcccg tgatccatgc caaatcgatg
     8941 agcttcaaca cattgtgata gccgtccggt tggcgcacga tgccttcggc gcaaagaacg
     9001 atcgatcgcg gcgattcagc aaagatggtc gccgcctctt tgaattgtgc gacggaaact
     9061 cccgttttcg ccgcgagttg ctcgagcgaa agagacgcca ccgcgctctt gagcgctgca
     9121 tacgcctgcg gatgtttttt cacgctctct tcgtcaacaa gatcaagctc caccgtcgcc
     9181 ttgacgaggc cgtcaatgac gagcccctcc gtgcccgcct tgatgagcac cggatgcgag
     9241 gcgagtttgc cgaggttggt gatggccgaa tcgaagacca cgacctgtgc cttgtagacg
     9301 cggatggctt ccttgatccg aacggaagtc aatggattgg tctcagtgag attggagccg
     9361 atgaccacca cggctttcgc cttggtcaag tcttcccagt cgttgggcgt gcggccgaca
     9421 cccagggcat gcttggaggc ccggacgaaa ttcatgtgac catagcgggc gctgctgtcc
     9481 agattattgg ttcggaatgc cactcgcatg agcttttgga acaaatagag ttcttcattg
     9541 gtacagcgag ccgtgatcag cccggcaatg gcgtccgggc cgtgcttctt ccggatgtca
     9601 gcgaaccggt cgacgatagt atgcatcgct tccagccacg ggacctccac gagctggttg
     9661 cccttgcgca agagcggccg tttcaatcga gccgctgaat cgatgtactc aaacccaaag
     9721 cggccccgca cgcagaggcc gccatgaccc ttcgcggtat cggcccgatc gccccacttg
     9781 tttttccagg acaaggggga cgtcacccgg atcacttccg tatctttggt ctccaggtac
     9841 atctggcagc cgtccccgca gtagttgcag gtggtggtcg ttttcttcat ctgccagggc
     9901 ttgtagaggt acttggagaa cttattggtg atggcgccga ccggacagac cgcaaggcag
     9961 tctccacaga actcgcaagc cagcgggagg tcgcctttgg ccaccacttg attgaagccg
    10021 ccctttttca tgaattgcag ggcgtcgatc atcagcacat ccttgcagac gttgatgcac
    10081 tcggcacacg caatacagcg gttcatgttg aagtccagca cagggcttcg cgtatcttcg
    10141 gggatgaact tctgcttcgc attggcgaga ttcgtcacgc catgctggaa ggccatgtcc
    10201 tgcagctcgc aatggccgtc ggcgtcgcac accgggcagt ccaagggatg gacggataaa
    10261 tgtttctcga cggccttctt tctcgcctgg aacagatcat cgccttccgt tcggatgatc
    10321 attccggccg tagccttggc cgtgcacgaa cggaccggcg ctttcttccc ctcctgcatc
    10381 acgagacaca tgccgcagga gccgaaggga tcgaacgtgt agtggtagca catggccgga
    10441 atgattttgc ccgtgcttga aatcacgtca tacagtgaga cgccgtcttt cgccgtcacg
    10501 gtcttgccat caatgctcaa ctcaatcgtt gcagcttcga catccggatt tgttgctggt
    10561 ttcaatccca tggtcttcct tttacttcac tccttttaca tgaggcgagg gctgggtatt
    10621 caacgtgcgc gcgtcaacga gggccttctg aggccgcgcg ttgcgcgagc acaggaatac
    10681 cttgcccgct agcctcatct gtcgcactct cccatgacaa tgtcataggt tccgaacagg
    10741 gtgatcgcgt ccgcaatcat atagcccctc gacatgtggt caaacgcgcc catatgaata
    10801 aacgacggag cgcggatctt gagccgatac ggttttccgc cacccgtgct gacgatgtaa
    10861 aagcccaatt cccctttatg cgcttccgtt ccacaataga tctctcccgg cggggcatcg
    10921 aagccttgcg aaaagagctt gaactgctgg atcatcgact ccaggttcgt aaatacgcgt
    10981 tctttgggcg gaagcgtgac gctgggaaca tccgccatga ttgggccgtc ctgaatctgt
    11041 tcaagacact gcttgacgat cttcacactt tcgtacaact ccatcaccct gatccagtat
    11101 cgatcgtagg tgtcgccgtt tttcccaagc ggaacgctga actcgcattt ggggtaggcg
    11161 gagtaaggtt ccgccttgcg caaatcataa tccaccccgg aaccgcgaag cgttgggccg
    11221 cttaagccga aattgacggc gtcctccgcg gaaatcaccg cgacgccctt ggtacgggcc
    11281 agccagatgc ggttcttctc aagaaagacc acgtactcgt caatcttaga gggaaaatac
    11341 tccaagaatt gtttgagctt ggcgaagaga gagggagtga agtcccgctc gactccgccg
    11401 atgcggtacc agctggtggt caggcgtgcg ccgcagagtt cgtcgaacca gtccagcagg
    11461 atctcacgat cacgaaaaca atagaagaag accgtcatag ctccgatgtc gagggcttga
    11521 gtgcccaacc agaattgatg gccaatgatc cgctgaactt cggccacgat cgttcggaga
    11581 tactcggcgc ggtcggggac cgtgatgttc atgagctttt cgactgcccg gcagtaggcg
    11641 aagttgttgt acatggcaca gacatagtcc agtcgatccg tgtgaggaat gaactgatga
    11701 tacgtcccct cttccgccag tttttcgacg ccgcgatgca agaagcccat cacgggcgtg
    11761 gatttgacca aacgctcacc ttccaattcg aggatcacct tcaagacccc atgggtgctc
    11821 gggtgctgcg gacccatatt cagcagcagc tcctcagtcc gtaaggtcgg aagcgtctcg
    11881 ctctcgggat gagcgggatc gactttatag acggtggttc tttggtcttc gaacgccatc
    11941 ggcaacgtac taccttgcgg tttcgtccag gaattcgaac gtgtcgcgcc agcccttccc
    12001 cctgagtgga aagtctttcc gcaacggata gccttcggtg tagtcgtccg gcatcaggat
    12061 acggcgaagg tcgggatggc gccggaagcg aatccccatc atatcgtaga cttcgcgctc
    12121 catgaagtcc gcgcccttcc acagatccgt gagtgaatcc accacgcaat cagactctgg
    12181 cacgcgcgtt ttgagacgga tacggtgccg tttcctgatg gaataaaatt catagacgac
    12241 ctcgaagcgc tcttcgtcgt ccggccaatc gacggagctg acgtggacga tgtagtcaaa
    12301 gtccatcgcc gggtcgtcgc gcaagaactg ggcgacctcg tgtagcgtgt ccctggtgac
    12361 ggtgatggcc agatcgccac gccattcgac ggctttgaca aagccggcgg caaagtgttc
    12421 ccgcacacgc tcggacaact gttgaagcga catgggattt agactttcaa gctggctttc
    12481 acttgctctg gttgcttgac gaacacacgc ttttgcatga tgcgttcttg cagcttgagg
    12541 atgccgtcaa acaaggcttc cggggtcggg ggacaaccgg gaacgtagat atcgaccggc
    12601 acgaaacggt cgacgccctg cacgacacta taactgtcgt aaatattgcc ggatgtcgcg
    12661 cacgatccca tcgcaatcac gtacttcggt tcgggcattt ggtcgtatat ctttcgaatg
    12721 accggcgcca ttcgccggca gacggttcct gccacgatca tcaaatcgga ttgcctcggc
    12781 gacgcccgga acacaccggc gccatagcgg tccatgtcat agcgcgagga gacggcggcg
    12841 atcatttcaa tggcacagca agccaacccg aacgtcatcg gccagagcga ccctttgcgc
    12901 gcccagttca ctgccttttc aatcgacgtc gtgatgacgt caggcatgcc gtctttgtca
    12961 tgccgtccca actggattag tcccattcca acgctccctt acgccaggcg tagacgtaag
    13021 ccaccaggaa caacgcgatg aaaatcagca tctcgaccag gccaatgaca ctgactttgt
    13081 tgatgtcgtt aaagaccacc gcccagggat ataaaaagat gacttcaata tcgaaaatca
    13141 cgaacaacat agcgaagata taatagcgaa ccgggaacgg catccgggca tcagaaaacg
    13201 gttcggaccc acactcgtac gcagacaact tttcaggttc cgggtacttg ggttgaacca
    13261 aatagctcaa caacagggtg acgacgccga acgccagcgc aataaaaata aacaacagaa
    13321 tcggaaaata cttggtcaga tactcaagga gtaactcaag accgctcatg gagttgacct
    13381 ttcaacacct tgagttgcaa ggagaatggg atattaaacg gggggttgcc gcgatagcaa
    13441 gcgcacaaag gacctaccgg ctcaagaccc tgaagtccta tcactcgtgc cgagtccgag
    13501 caggcgccga acgcatgagt gccgaatccg ttcggaggtg gattgggggg gtgccgtctt
    13561 gcttttccca ttctgggagg agaggggaaa aagccttcgc ctttttccgg cgatgaattt
    13621 gagtctccaa gcgggggaac gtcacctgtt tctgtttacg cggcatcgga tcctcgtcac
    13681 ggcctcagga gtaatacgtg aaaaccatag caccggcttc tattgagggt caatgggggc
    13741 aagatggcaa tatccttgac tgtacaggac tatttgctat ggtcatgtgt gacgcttcgt
    13801 cttaacagaa ggaccaccca tgacgactct accatcacgc accttcctga gctgctcgct
    13861 gctcgcggta gtcagcctca cggggctccc ccacgcgggg gccgatgact tagacactat
    13921 acgttacggc gaagcggcac gcacctatgc tgcgggatcc atccaacagt cgactcccca
    13981 agacggtgtg gtcaacgtca tcaccggtga caatcaaacc gtcggtaatc gcatgcgact
    14041 cggacagaac gatgtcctct atctgaagtt gaagcacccg ggtgaggctg cagtcggcga
    14101 tctctacacc gtctataagc ggacccggaa agtgtttcat cctgcgacag gccagtacct
    14161 gggctacttg atcacccggc ttgcaatcgt gcaggtcgtc caagtggaca aggaattaac
    14221 cacggtccgg gccgtgcgtg cgttcggcgc agccgccccc ggtgatccgg tcatgagatt
    14281 cgccctgcca cataccgatg aggctgccgt catcccggcc tcaagcgatg tgacgggcat
    14341 ggtcgtcgaa ctgcaagccg atttacagat gacactggtc gcccagcgca acattgtgta
    14401 tctcgatcgc ggatctgacg acgggttgaa accgggagac cgcatggatc tgattcgata
    14461 tggcggcgcg ctgccgcctc ggcaggtcgg agaagtcaaa gtcttgtcga ccgaacagca
    14521 cagctcaact gcactggtca caaaatcagt gtcccgcatt ctcaaaggcg accagttcca
    14581 gctgaaatac cagtcggacc tcatccctcc agcaacgcca acgaagcggt caatggatgc
    14641 ccctccggcc ggacactcgg gagactccat acccgttcaa aacgctgcgg gcgaaacccg
    14701 gtacagcttc accgatctca tgaaacaatt gcgctatgaa tcaggagaag cgaggatcac
    14761 gcccgaaggc tatgtcacgc tcagtcggct ggttgagctc gtgaaagccg ctccggccga
    14821 tcagttgatc cggatcgaag ggcatgcgga taacatggaa atcggccctt cgctaaagtc
    14881 cgtgtatccg acgaattggg acctctccaa ggcgcgtgcc accggggtcc tgcggtattt
    14941 agtggaaaaa ggcgggattg attccgcccg agtgtcttca gtcggctatg gcgattccaa
    15001 accgatcgtg agcaatgcca ccgaggcggg ccgttcgaaa aatcgacgcg tcgacattgt
    15061 cctgtattct cccgactcca ccagtcacac tcgttcggag ccgacgaacg ccacggacga
    15121 cggataccgt gtgtccggcc ttggtgccga cgaacagaag ccggcagtat cagccgttga
    15181 ccacgcgtcc tctacagaag ccgcggcatc ggaacaaccg gtttcggtcc ccgctcaggc
    15241 cacaccggat cttcccggcg gcgaaggcca cgcggtgcct gcgccgatcg actcaccagt
    15301 gccagtacag ccgtaacgtc gtttatccag gctggagcat gcgacccccc gcatgttcca
    15361 gcaagatccc gccggctcat tgttccacct tcccgcggtc gctgctacaa tgccgcatga
    15421 ccggtgacgc gcggctctct acgccgattg agaccgagac gctctatgcg gatgtgattg
    15481 tcccgcgtca cctcacccgt ccattcacat acatggtgcc cgctcatttg cagtcggttc
    15541 tgcggatcgg gcacgtcgtc tcagtcccct tcggtcgatc cctgattcac ggggcggtca
    15601 ttgcggttac aggcaaccct ccggcaggat tggaccgcca gcgcctgaag tccatatgcg
    15661 ctctcatcac ggccggccgt gcctcagaga tttccccttc cctgcttgaa ctggcccgat
    15721 gggtggctga gacctatgtc gctccctacg gacagtgtct gaggttagta cttcctccgc
    15781 gtgcgaaaac gcctgatcga agccgcatcc ttctgaccag gaagggggaa gaggctcttg
    15841 cgacaggcgg attgccgact caaaccgagc tcgagctgct caaacgcctc aagcgacggc
    15901 cattgggcat tccgctgagc cgccttcgag taggcaaaac ccgatgcccg gaggacgtgt
    15961 tggccgcgat ccttacccga gaatgggtcc acaaggtccc aggccgccca gccagtcatg
    16021 ctgtcccccc acagccactg atgacgtcgg gcagtccgga tctctggcat gaccaacaca
    16081 aggaggcctc ccaagccatg gagccttcgc ttccggaatg gcacgcgcgc gtggacgagg
    16141 ctctcggcag acgagaggcc gcccgtctgc tggttgaagc cgctcctggc gtacgcctgg
    16201 ccttgcttcg ccatgccgtg gacagaaccg tcgctcttgg gcgcagggtg ctcgtcatca
    16261 ccggtgaaac gacgcgcgca gaatccctcg cgctcgcgct tcatgatcca acgcgtgtga
    16321 caacctgtct gcacagcacg atcccagacg agaagaaggc ggacatttgg aatttgatcc
    16381 gcgaagacaa ggtctcggtt gtcgtcggca ccagatctgc tgtgtttctc ccactcccct
    16441 cgatcggatt aatctggatc gatcgagaag aggatcccgc tctcaaagaa ccgatggagc
    16501 ctcgatatca tgcccgtgat gtggcctgga tgagagcccg ggaggaacag gcgctcctgg
    16561 tgttctcttc cgcacatctc tcattggagg ccacggtaca ggcacccgag catgtgttgc
    16621 gcgcatccgt tccacgggag gctaccccat acattgaagt cgtcgacctc cgccgccacg
    16681 atcgcacggt tcttttgagc cccaccctga ttcaaaccat gcgggaagcg atggtgcggc
    16741 aggcgggcgt cctgttgttt ctcaatcgaa aggcgtacgc cggcgcgctg gtctgccgag
    16801 attgcggtca ggttccccgc tgtcgatctt gcaccgtcgc actggcgtat tcacggcaaa
    16861 agcgctcggt gttttgtcat tactgcggag ccgccgatgc gattcccgat ctctgcgcag
    16921 cttgcggcgg gccacgactg caacctattg gggaaggcac agaacgggtt gaagaagaag
    16981 tgaagcgaca gttccccttg gcccgcatcc tgcgggtcga cggcgagacg atgcgaagag
    17041 cgaaggacgc tgctgcgatg tggaaccgga ttcaccggcg cgagtgggat gtcctcatcg
    17101 ggacacagct cctcttgcgt gacgatgtgg tgccgcgggt tggcttgctg ggcgtcgtgc
    17161 aagccgatgc cgggctcagc gttccggatt ttcgagcggc tgaacgcacg tatcattttt
    17221 tactggacgc gtcccggctc gtagagcctc gtggaaccgg cggacgtctc attattcaat
    17281 cctatcttcc cacacaccac gtcattgagg ctgtcgtcca gcaagaagag ggacgcttcc
    17341 gaaccgaaga gctggcacac cgggaagcgc ttggctttcc tcccttgctt cgtttgatcg
    17401 ccctccacat ctccggtcca gcggagcgca gcgtcgcgca agcggcgcaa gcctgggcta
    17461 cccgactgag aagtcttgca gcagtcgcac gtcccggctc acaggacgga gcagccgctt
    17521 gtgctatcgg ccagacgaat ggcctgacgg tattgggacc ggtcctgtcc cccgtcgcgc
    17581 gcgtgcgggg gcgctatcgg cgacaaatcc ttgtgaagtc cgctgacgcg gccagcgccg
    17641 tcgaggtcgt tcgatcgacc ctcgctgacc tggagaccac ctatccggcg agacaagtga
    17701 aattcgacgt cgatgtcgat ccaatcgaga tgtggtaagg cgttaccgct tcctctgccg
    17761 gcgaggggat tgcgtctccg gggattgcgg cagccggtta ggatcagtgc ctttgaataa
    17821 gccgtaagag aaagcgatcc agaagaccgc agaaaacaag cccatgagca cagccgagag
    17881 gatcgtgccc atctgctcat ccgatggttg agtcgtcgcc gatcgaatgc gcagcatggt
    17941 gatgattggc cacgccaggc attcgatgcc gaggaaaagt gtggtccacg cccagatcag
    18001 acggagcgtg cggccccgcc acacgagaaa gcacccggcg atcaccagga tcatcagccc
    18061 gcccacgccg gctaatccct cccaagcaag ccaggcgccc atcgcgacga cgaggcttcc
    18121 gagtgccgca ttgatgcgaa gtgtccacga tgatgccatg aggtcgctca gtgtgcttgt
    18181 ccgtggtggg tgcaggcgcg tcggtgacag ggtcggagag gggcctgctt acacggtacg
    18241 cttgacaaaa aatgcggccg cctgggcccg acgggagata tggagttttt gaaacatgtt
    18301 ggccaggtag tttttgacgg tcttgtcgct cagtttgagc gatgtcgcga tttctttatt
    18361 cgttaacccg tcggcgacta aggccaacac ccgttcctcc tgaggggaga gggaggatgg
    18421 acggaccgga ccttcggccg ccgggaccgt cttgatccat tcgagggcgc gcttcgtgac
    18481 gttaggatcc aggatcgaac ggcctgcaga gaccgtccga atggcgcgaa tcagcgcagc
    18541 ggaatcaatc tccttcaaga catacccgta tgcgcccgcc aagacagcgg ccagaactga
    18601 atcgtcatcc gtgtaagacg tcaggaaaat cacgcgcgag ttgggctgtt cggagaggat
    18661 ctcgcgacag gcttcgacgc cggacccgtc gggaaggcgg acatccatca gaatgacgtc
    18721 cggcctcagt cgcttggctt caattgccgc gtgagccttg ctgtccgctt ctccgacaat
    18781 ggtgatgccc tggttgttgt gcaagaccgt ccgtaatccc acccggacga cttcatggtc
    18841 atcgacgagg agcagcctga ttgaagaagc tttcggggag ggaacacctc gtcgccgtgg
    18901 cgacgtcaca cttgcaacag tcatcgcagg ttcccgtaga gaattggtgc cgaaggcggg
    18961 acttgaaccc gcacggcttg cgccacacgc ccctcaaacg tgcgtgtctg ccattccacc
    19021 acttcggctt agggaatgtt gaaaatgccc atcagcttcg ttcttggtcg cctgccgacc
    19081 ggtggcatgc cgaccacctc gcctcatttg acggcttcca gggcctcgtt cacgggcatt
    19141 tgaacgttgc caatgcgatc caatcaacgg tcttgtgagg gaggggcatt atagaagtag
    19201 gccaaaatcc ttgtcaatca atggcctgtc cggtaatatt ggccgtctgt cgctcgccag
    19261 ttgatccaat accatgcaag gctatcacga tctccatccg tcactcgtcc gggctgcaac
    19321 gaccgcatcc cctgcgtcgc ttgccgccct cttcgacgtc gataatacgc tcgtgccggg
    19381 agaagcctgc gaagttcggt ttttccgcta cctgtggcgc cgcggtctcg tcggatggcg
    19441 cgagttgagg cgcagtgtgt cctggttggt cacacagggt cggtccgtct cgatccatcc
    19501 gctacgggag cgaaagatct atctcgaagg aaagaattcg gcgctgatcg agtcctgcgc
    19561 gcgggatttt tttcaacgcg aaatggtcag aaacatctcg ccgaaggggc gcgaccggct
    19621 cgaagcgcat cgccagtcgg gccatcagct gattttagtc tcgggtgcgc cggactttct
    19681 catcacgccg cttgctgaga cgctgaacgt gtccacgctc tttgcggcac ggattgagtg
    19741 tcaaggagca tgctataccg gcgccgtcat tccaccgctt ccttacggca ttggaaaacg
    19801 gcagctgatt gtgtctcatg cccaggaaaa gggtctcgac ctgaccagtt cgttcgcgta
    19861 cggtgatagc cctggagata cggaaatcct tgacctcgtg gggtatcccg tcgtcgtcaa
    19921 cccgattcgc ggcatgcttc gcatcgcgcg cgaccgcggg tggccggttg tggactggac
    19981 ctgaccatgc gtgtcaccga aatctttcac agcattcaag gcgaatcgag ttacgtcggg
    20041 cagccttgtg tcttcgtacg gttgaccggt tgtccgctcc gctgcacctg gtgcgacacg
    20101 gaatatgctt tctatggagg aaaggagcag tcgttagagg agatactgtc tcaggttgaa
    20161 gcctatggat gcgagctggt ggaagtgacc ggcggtgagc ctctcgcaca gcctgatgcc
    20221 atccccttga tttcgacact ctgcgagcgg ggccacaggg tcctggtgga gacgagtggc
    20281 gccatcgaca ttgcaccggt cgatagccgg gcgcatgtca ttctggacgt gaagtgcccg
    20341 ggcagcggga tgaccggacg gatgcattgg ccaaatctcg accggctgac gacgaaggat
    20401 gaagcgaaat ttgtgctcgc tgatcggacc gactatgagt gggctcgtga catgcttgtc
    20461 cagcatgcgc tgccgggccg ctgcaccgtc ctcctgagtc cggtgttcgg tgcccttgat
    20521 ccgcgtcagt tggcggaatg ggttttggcg gaccggttgc ccgtccgatt gcagattcag
    20581 ttacacaaat tgatttggac gccggacatg cgcggcgtat gagccatcct tcctgctcgc
    20641 caggatagga gcgcattgac cggtgatgac tgacaaggag tagacggaat gaacatcatg
    20701 cagattgacg ccaaggtcgg gcgtgcggaa acggaagcgg cagaaatgct cgtgctcgcg
    20761 cattgcgaag gagagcgtct caacaagcaa gacacttccg cactcgacaa agccctgggc
    20821 ggcgcattaa gcgagctgct gcagtcaaaa gagttcgaag ggagagctgg ggaaatactg
    20881 gtctatcaca cgcagggtaa ggtgccgtcg aaacgtctcc tcttcgtcgg cctgggcaag
    20941 aagaacaact tgacgctcga tgccattcgt caggcaatgg gtcatgccgt gaaacgggtg
    21001 cggcagacga aggccggtgc ctttgctgcg cttgtgccgg cgtggactcc gcgaggacat
    21061 tctgctctcg aggtcgcgca agccatgacg gagggggcga ttctcggtca ttatcagttc
    21121 acggcctatc gtagcgacaa tggcacgccg acagaggtcg gccggatgac gctgctggcc
    21181 ccgcagaagg ctcagttgcg gcaactcggc gaaggcatcc gccgcggcgt ggcaaccgcg
    21241 gaggccaccg tgctggtccg ggacctctgt aatcatcctt caaacgtcat gaccccgtcc
    21301 cgcatcgcca atgaagccaa gaaagtggcg aaagaggagg cgctgagcct caagatcctc
    21361 gaacaacgcg atatggaaca gctggggatg ggagcgttat tgggggtcgc acgaggcagc
    21421 catgaaccgc cgaagttcat cgtgctcgaa tacaacggct ctaagaaaaa gaacgagcgt
    21481 cccgtcgtct ttattggcaa gacgattacg ttcgacacgg gcggaatttc tctcaagccc
    21541 gccgagaaca tggagcacat gaaggccgac atgaccggag gggccgaagt gctggctgcc
    21601 gtcagggccg cagcacgtct tcgattgcct ctgcggctcg tcagcatcct ccccgttgcg
    21661 gagaatatgc ctggcggccg agccatgaag cccggtgaca tcgtgaagac gttatcgggt
    21721 aaaacagttg aagttcaaaa tacggacgcc gaaggacgat taattctcgc cgatgcgctc
    21781 gcctatgccc aacgctacaa cccggccgca atggtcgata tcgcgacgct caccggggcc
    21841 tgtgttgtgg ccctgggcca attcgccatc gggctattcg gcacagatga gaaactgaag
    21901 gcgcaggtca aaaaatcggg cgacaaggct ggtgaacgcg tgtgggagat gccgctgtgg
    21961 gatgaatatt tcgaacaact tcgcagtgac gtcgccgaca tgcgcaacat cggcggacgc
    22021 ggcggcggca tgattaccgc ggccctcttt ctgagtaagt tcgccggcga tggcccctgg
    22081 gcgcacatcg atattgccag taccgattgg agcgagcggg agcgggcgta tattcccaag
    22141 gggccgacgg gcattggaac aaggctcttg attcaatatc tcattgaccg gagcctgtga
    22201 cagtggcccg tctcgagctg acgtcgcggg aaaaaatcgg gcagctcttc atgatcgggt
    22261 tcatgggaac caccgtcaca ccagagctgt ctgcattcat caaagactat aaaccgggtg
    22321 gagtgatcct gttctcacgg aatctcgaat ccgttgaaca gattgttcag ctgaccaacg
    22381 atctccaaca gtgcagtccg aagtctcccc tcttgatctc catcgatcag gaaggggggc
    22441 gcgtgtcccg tctgccccaa ggctttacca tctttcctcc ctgtgagctc atcggacgat
    22501 gcaattccag cgaactggcc tatgcggctg cggcgacgat agccaaggaa ctcaaagccg
    22561 ttggcatcaa catgaacatg gctcctgtgc tcgacgtcaa tagcaatccc gacaatccgg
    22621 tgatcggcga ccgggcgttc agctcctcgg cgcatgccgt cggtgaaatg ggactgatca
    22681 cggcggccgg attgcaagat caggacgtgg tcgcctgcgg caagcatttt cccggccatg
    22741 gggacacgaa tgcagactct cacaaggaac tgcctgtggt tgaggcttca cgggagcgat
    22801 tagaggccat tgagtttccg ccgtttcgcc gggccgcggc ggctgagatc gcgacgatga
    22861 tgaccgcgca tgtgttatac aaggcgcttg atgaagcgct tccggcaacg ctgtctccgg
    22921 ccatcatcag cggtcttctg agagaacaga tgggctatga cggggtcgtg ctgacggacg
    22981 acctcgaaat gcatgcgatc gtggaccact acggcccggg agatgcggcc gttcgagcgc
    23041 tgctggcagg gtgcgacgtg ttgttgatct gtaaggaacg tgaccgcgag atcgcggcct
    23101 ttgaagcggt ggagaaggcc gtggcatcgg gaactgtgac gatggcgagg ctggatcaat
    23161 ccgtcgcaag gattcaacgg gtcaagcaac gctatcttac accgtaccgg ccggcgatga
    23221 tctccgaggc aaagttgatt gccggctgcc ggacgcatca ggcgcttctt cagagcctgc
    23281 gtcaggcgag tcaccgcatg gagaaacgct cgatagcgag tagcctctag ttgatcaaag
    23341 gaacgcgggc cgatcatggg ggtcaaaaag ggagatctga tcgatgaacg taaacggcat
    23401 cccgactctt gcggcctcgt cgtgaaggtc atgggcggag gggaagggtt tgagaagatc
    23461 gtctgttgcg accatgaatt gaccgagctc gatgtggtgc cagcgatggc gagtgccaaa
    23521 gggcgcaagc gtggtcagct ggccacgggc gtcgtccttg atgaaaagaa actgtatccg
    23581 gactcctgcg ggctgcgggt catgatcatg gatggcggag cgggcttcaa agaaatccgg
    23641 tgctgcggcc attcgttgac ggtcagttcc aagcgggagt tgcgctatga cctgatcaga
    23701 gggtcagatc cgccccagaa cgggccggaa gggaatgcat agtccatcgt caggagggct
    23761 gccatgcagg accgtaagct agtcatgaaa cgctggtgtg gagtcatgga atggctcgac
    23821 cggcttggct atctgacagc aggcttcagt ctgctcgtcc ttggtatgct catttttgcc
    23881 catgcctggt acgtcttcat gacgaaagcg ggcagcagcg ggctgttgcc ttccggcttg
    23941 aaactcttga acgatctgct actcgtcatc attctcctcg agctgtttcg cacggtcatc
    24001 cgcttcctgc agactgaggt cctggatctg gaaccgtatc tggcggtcgg gattatcgcc
    24061 tgcaccaggc gtgtccttac ggccagcgct gaattgagcc acctgccgaa catgaccgaa
    24121 actcagttct atcaatatct catggacgtc gggttgaacg tggcggtgat catggttctc
    24181 gttgtgggcg tgttccttgt ccgcaaacgt ccggctcaag ctcttcccgt ctcgccctag
    24241 tctccaattt ctccctgcgt gtgatcgccg cgaagaaccg acgtccgttg agcccgctgc
    24301 ttgcctccta tttcggctta tggcatcatg cccctccctg gcatggcgaa cttactggaa
    24361 tacgtcggtt ctgtgcacat ttatctcggc ccttataggg gaaatccgat cgcgctctac
    24421 ctcaaacgct ctgacgcggg ctgccagatc ggcccgaaag tctatccgtg gaacgacgtg
    24481 gtggggatgg gggattcgcc gaataaggcg gcggcggatt tcgaggagaa atggaaagcc
    24541 aagggtcttt cagccgacat gtattcaggg ccgtcatggg aaggcggcat taaacccgaa
    24601 agaccggctc ctcccaagcc gccagcgact ccgaaaccgg ctgctcaagc gaactctgct
    24661 cctgcttcgc aggcttccgc tgcgcctgcg gcttcaactc ctgcccgggt cccgtcgacg
    24721 ccttccggtc agtcgtccgg cgaatcaagc tagaattcag cgcgacctgg cgttcagcca
    24781 ggccgaccac agtgctgcgc ctgtcttcga gaccgctgcc cctcaagacg ccgaagtctg
    24841 cttcaacgcc tttgccgaca atcggctgtg gttgagacca taacttaaat agacgacgat
    24901 gccgatgacg gtccagacgc cgaagcggat ccacgtcagt tgggggagtt gccacatcag
    24961 tcccagacag gccatcgtgc caagaattgg aaccaacggc atgaacggca ggcgaaacgg
    25021 tctcggctga tccggtttgg tatatcgcag cacgatcaca ctgatgcaga cgagcacgaa
    25081 cgcgaagaac gtcccgatgt tggtcatgtc ggctgcctcg ccgatgggaa tgagcgccgc
    25141 caagatcgcc accgcaacac cggtgagaat ggtcgcgcgg tggggagttc cgaaccgagg
    25201 atgaaccgcc gcaagccagg ggcctaacag ccggtccctc gacatcgcaa agaacacgcg
    25261 aatctgaccc agcatcatga cgaccagtac gctggtaatc cccgcgaccg cgccgatcgc
    25321 gatgactgcg gccccccact tgaaacccgc caatctgagt gcctcggcca ccggagcatg
    25381 gatatcaatt tgcgatgagg gaatcaggcc ggtaagtacg gcggcgacag cgatatacag
    25441 gaaggtgcac aggctcaacg atccgataat cccgatcggg acgtcacgag ccggattcct
    25501 ggcctcttct gcggctgtcg agaccgcatc gaacccaatg taggcaaaga acacgatcgc
    25561 tgcggctgct ccaacccccg ccatgccctg cggcataaac ggcgtccagt tctgcggatt
    25621 gacggaagtg ctgccaatcc cgaggaagaa caggatgacg gcaagtttaa tgagcacgac
    25681 tatgcccgtc gcgcgcgcgc tttccttgat tccgacgaca aggatgatcg tgacgagcag
    25741 cacgataatg gcagccggaa ggttggcgat tcctccatct gtgccaccgg gcggacgggt
    25801 cgcccactgg ggtaaatcga gaccggccag ttgcagaaga ttgttaaagt acccggacca
    25861 gccgatcgcg actgcgacac aggcgacgcc gtattccaaa ataagattcc agccggtgag
    25921 ccaggcaagc atctccccga gcgtcgcata ggaaaacgtg taggcgctgc cggcaaccgg
    25981 tatcatcgcg gacatttccg cataacagag cgcggcgagc gcacaagtga ggccggacag
    26041 gacaaaggac aggacgatgc cggggccggc tccgggccga tgggcatcac cgacaatggc
    26101 tgtgccgatc aggacgaaaa tgccggttcc gatgatcgcc ccgatcccca ggcaggtgag
    26161 atcccaggcc gtcagggact ttttgagtcg atgcgtcgga tggtcggcgt ccgcgaggat
    26221 ttgatcgatg gatttcgtgc gaaacagttg agagatcacc gggcgtctgc ccgcagcagg
    26281 tcgtgccaat gacggtcggc tgaggacgtc ttcccgctcc atgttccatc aagtatagca
    26341 ggccgtctca caaggaaatg gaagattacc ggcatagcta gctcgtgcct ttccgtgcag
    26401 cgtggagaaa cgcctcgaag agtcgccgat ggagcttgtg ttggtcgaac agaaattctg
    26461 gatgccactg aattccgaga aagaaccgct gggacggcga ttcgatggct tcgataatgc
    26521 cgtccggtgc cacagcgctg gccatcaagg atggcgccac gtccttgacg gattgatggt
    26581 gagaactgtt gactcgcatc gtcccagtct tgacgatgcg ccgcaacagg cttccggagg
    26641 tgattgtcac gtcatgactg agcctggttg cgggagtcgg ttgtctgtgc tgcagcggct
    26701 tggatcgctg ggcggcaata tcctgataca ggctgcctcc aaaggcgaca ttgagcgcct
    26761 gcatgcctcc gcatatagcc aacaccggca tattggcggc gcgcgccaag tgagccattt
    26821 ccagctcgaa tgttgtgcgt cgttggctca tcaccctgaa cttgtaccgc tgacgttcgc
    26881 cgtagagttc cggagcgaga tcgggtccgc taccggtcag caagaggccg tcaatgtgag
    26941 tgagcaaacg ccgccgagct ccaaggtcgg cgaccagtgg aaggacgacc gggattcctc
    27001 cgaggtcttc aatggcgcga acgtaacgag cgcgcaagaa ataggtgggt tcgcgtccgc
    27061 cccattcttt tcggtcaccg gcgttaaaat ctggtgtcac gccgatgagc ggtttcattg
    27121 ctcaacgaat gggctccatg ggggccgcgg cctgagccga tgcctccgtc tccgggacgc
    27181 cgaggaaacg gatgggtctg tctccgcgca gatgatcggg cgtgatgata tacgtgccat
    27241 agaggctcgt ggtccagata ctctttgccg cgttctcgtt tcctccggaa aacgcatagg
    27301 ccagcccgcc tacgatgcct cctccgatgg caaaggccgc cttcaacgga agatacagca
    27361 aggttgaaac cccggcagca gcgcccatac cggcgctgga ggcgctccca ccttcggtcg
    27421 tctcgctgga tggcggaagg gactcttggc tccacgccga gggcgccacg ctgaccatgc
    27481 caagagcgag gacgagaaca acggcgacga gcctgaacgt tgccgcagtg taagcacaca
    27541 catataattt cacgagaggg tcctccttcc tcaacaaagt gaatcgagct gcatccgtgg
    27601 aaattccttg ggcgctcagt cgaagtgcag cgtgctgtgg gttataacaa attacactgt
    27661 gcttgcaaac cccctaccag gtcggaaaac tcgccatcaa gcgggatggt gcggaaggag
    27721 atcaagttct gtcgccacat gtgatgcgac gaagtgccgt ttgttgatag caaccatgtt
    27781 caattcggtg acaaatgtgt cggcggtgat acattgattg ttctgagtga tgccggattg
    27841 caacaataag tctaatttgc accaggcgag cgcttggaca aacaggtctt gcgcccgctc
    27901 ctggtaagcg ggacgctctt caaccggaac ctgcacaagc gcctggtcca gccattcagg
    27961 aaagccgatg ctcgaactca gagaattgat ctgagattcc tcgagttcga cggccacctg
    28021 gacgccacct ttccaactcc gctttttgac gttgtacacg gtcgagtaag tgaagggttt
    28081 ccccttcacc ggctgcgggc caatgaacaa tgtgatgcga aattgagatg cctcggaatc
    28141 tatcgacatg ctgcgcattc taccatggcc gatggccatc cctcttcatt gacgctccaa
    28201 agccacaacg ataagatgcc gaccatgccg gacctcgcgg gtgaacagcg aatactcgag
    28261 atccaacggg tgcttcgcga acatgattcc acggacgggt ggctcttcta tgatttccgt
    28321 ggaagcgatc tcctcgccta ccgtgtgctt ctgctcgatc cgtgtatgca tgtgaccagg
    28381 cgctggtact actgggttcc ggctcatggc acaccgcaaa aattggtcca tggaatcgag
    28441 ccgcacgtat tgggttccct acccggcgat acgagggcct atgtgtcgtg ggaccagcag
    28501 cggcaagagc tcggtcgtat tctcaccggc aaccgccgga ttgcgatgca atactcgccc
    28561 atgaatgcgg tcccttatgt gtcaagggtg gatgccggca ccatggaatt agtgagaagc
    28621 ttcggagtcg acgtcgtcag ctcggcggat ttcatccaac tattcgaagc acgctggacc
    28681 gaccgccagt tggagtcgca tcagtacgca gccgccgcat tgaggcgcat tgtggacgac
    28741 gcgtttggac atgtcagtca gtgcctgact catggaacgc ggctcacaga atacggctta
    28801 cagcagttca ttctctcccg gatcaaggac gcaggcatgg tgacgtccag cccacccatc
    28861 gcggcggtga acgaacacag cgcggatccc cattacggtc ctacagcgat ccgatcctcg
    28921 accattcaac gagacaattt ggtgctcatt gacctgtggg caaaacaggc tgacgccggc
    28981 tccgtgtatg ccgacattac ctggacggcc tatgccggtt cgacggttcc cgagaagcat
    29041 cgcgcagtct tcgatgtggt gaaaagagga cgggacaccg cgcttgaatt cgtccgggag
    29101 aacctacggg ctggctaccg accgttcggc catgaagtcg atgctgcgtg ccgccgcgtg
    29161 attcaagcgg cgggctacgg agatcaattc attcatcgta ccggccactc catcggcgaa
    29221 gaagtgcatg ggaacggggc gaacatcgac ggcctggaaa cccacgacac gagacgcctg
    29281 atgccgcgca cctgtttttc gattgagccc ggcgtctacc tggctggcga atttggaatc
    29341 agaagcgagt tggacgtgta tctgacggaa cacgatgcgc tcgtgttcgg gcttccgcta
    29401 caaaccgcgc tttccccatt gctgtaggcc cctccagccg catccttcct tgacagtgct
    29461 gtcaaacggc ctataaagtg actgttggct tttaggccga tactcctgaa acagaaggaa
    29521 taagagcatg ttcggcacca tgggcatttc agaattgatc attattctgg tcatcgtgct
    29581 gatcattttt ggtgcaggca agcttccgca gatcggtgaa ggcgtcggca aggcgctgaa
    29641 aggatttaag aaagaagttc acgatatccc tgctccggaa gcgccccagg tagacgccgt
    29701 gtccacggca gcttcaacga cgccacacgc tccagtcatg gcccaggctc ctgtgcaaca
    29761 agcagccgcg gcgcccccgg caacggctac caccgctcaa gccgctccta agccgacttc
    29821 gccgtacacc cctggtcccg aattgacacc cggcacgacg gccgcctcgc tctataatat
    29881 ggggccacag gcgccccaac cggcccggcc gacgaccgct ccagctggga cacaaaccgc
    29941 tcgagtcgct cagccgccga cgatggaaga gcgtgcagcg gccccgacgc ccatgatgcg
    30001 tggtcaatat cctccactcc cgccctctgc ccagcagaaa ccgcaggcta aacgaccgtc
    30061 ggcgattgtg aacaaggatg ccgtggctcg cgtgcaggcc cagcaggcgg ctctgaaggc
    30121 gaaggcaaca caaacgggcg gcgtgtcgcc tgccgacatg caacatcttg gtgaagggct
    30181 gggcgacgcc ttgcgcacgt tcaagcaggc agtggcagac gtgcggaatt cagttgatcc
    30241 cgagatgcgg acaattcaag ccgaggtgga cgcggctcag aaggagattc agcaatccat
    30301 cgaggctgct cgagaaatgc ctgccgtcca ggacgaacct ccaaaatcgg cctgacgcat
    30361 ggatgaatgg agctcgtcac gacgctcccc tctttccctc ttcctacttt tttgtctgac
    30421 gttttcgggg tgtcttcaag aagcaccctg gccatccccc gaccaaagca aggcactgct
    30481 gaccgtattg ctcaaggacc acaatcccga agtgcgtcgg acggcggtcg agtcgctggg
    30541 taaaattggc gatccaaccg cagtaccctc tgtggtgccc ctgctgaccg acccgattcc
    30601 gctggtacgt ggcgcggccg cgcaagcctt gggtcgcctg gcgaccccgg cggacgaggc
    30661 tgcagttgcc gcgttgaccc aagcgctcgc agatccggcg gacaatgttc ggcaagccgc
    30721 cgcgatggcc atcggcgaca tcgaaccctc gcctcgccag cttgcggtca tcaccacgct
    30781 ggcgcaggct gcagacgtac gagtgaggcg caccgtcgtg cacgccttgc agtccctgga
    30841 tacgagtcat gtggcgtcag ccctgctgcc actcgtgggc gattcggacg cagaggtgcg
    30901 gcagggagcc gtcgcgtgct tcggcaattc tgacgatgtt cgggccggcc gagtattgaa
    30961 acagcggctc gcggaggatc catccgccgc cgtccgtgct caagcggcct atcacttggg
    31021 agaagtgcgc gggatcgacg ctcgctccgt cttgcaggct gccgcagcaa cagaacagga
    31081 tagtggtgtg cggaggtgga ttgaagcgga actcaggtcg ttgcgcggga gcgattgagc
    31141 gcggccacca attcgaccaa atcgtgacgt gatttttcct cgatatcggt gaactgaacg
    31201 cccatgccgg ggaacaacag aaatcgttcc ggcttgttgc gtgtccaggc cactttcgct
    31261 ttcgctttgt gcttttccca gggacgatct ggaagggcaa attcgatgga cagttcagtg
    31321 ccgggtgcta aaggctgact gctttcaatg aacaggcctc cgccgccgac gccgccggtc
    31381 aagctgtcga attgcttacc ttcgggcgtg gtgtatttta ctttgaccgc cagaggggcg
    31441 cgggcatgcg cgcgcgcatg cgcaaagcgg atatcttcat cgcccgccga aatgcgttcg
    31501 atgacatcat gccaggacac tgctccaagc aaggcgccat ttgccccatg aagagaaatg
    31561 atttcgcggc ttgggtcaat gtctaacgac tctccctgat ggcccggagt cacagtgacc
    31621 ggaaatttca tgttggtacg ctctcttgac tgacagtgcg atgcaatgcc tgcatgatgc
    31681 tatcaggcgg caggcctacc gacttcctgc agggacttca cgagttcgag gacacgattg
    31741 cggatgtcgg cggcgatgtc ggtaaatcgg acgcccattc ccggactgaa cgtgtattga
    31801 tccgccttag ggcagaccca ggcgaccacc cctttcgccg cgagccattc gttcccacgc
    31861 tccggcaggg aaaactccag cgataacttg gtgccaacgg ggagtggaga ctggctctca
    31921 atgaacagcc cgccgccccc gatgccgccg gcccggctct cgaattgctg accttccggc
    31981 gttttgaact tcacacgaaa tgcgagcgtt actcgtggct cagtccgcgc ttcacgggat
    32041 gcgggtggtt tgcgataggc gaggatctga tcaatgacaa agtcccacga gagattcccc
    32101 agggcgtctc cgttgatccc gtagagcgtg atgatctccc tggcagagtc caattcgatc
    32161 gtcttgccct tatggcgcag gctggaaacc accggatatt tcacggatcc tcccctcgat
    32221 tgcccatcct cggcaagtat agcgcagccg ttcatcttgt cgaggaaaag ccgagagttt
    32281 cgcaggcatg attctgcgta ggctggtgtg atgccaccga attcagatga tccgaatgga
    32341 aacgctcgac tcggtgaccg gcgtcgcaga aaaaccgggc ccggtcagag gccgggccgg
    32401 tgagagtgaa gcaattgaaa tataagggat caggcggtcc tgacgtcctt gtcttgttca
    32461 aaaatgcgga tgggcgggtg tttgccgagg atggcatctt cggtgatgaa gacctccttg
    32521 atctgctttt gtgacggcgc atcatacatc acatccaaca tgacttcttc caaaatggcg
    32581 cgaagtcccc gtgcccccgt tttctgagta aaggccttcc gagcgacagc acccagcgcc
    32641 ccttcagtaa acttgagctt gaccttctca aacgaaagca atttttcgta ctgcttggtg
    32701 agggcgttgc gcggctccgt aagaatctta atcagcgcgc gttcgtcgag ttcgtcgagc
    32761 gtggcgacaa cgggcaaccg tccgataaat tcaggaatga gcccgtactt cagaaaatct
    32821 tcgggttgca cgcgctcgaa caattctccc aaccgcactt cggcgcgccc ccggatttct
    32881 gcgccaaatc ccatcgcttt gcgattcaag cgctgctcga tgatttgttc caatccaaca
    32941 aaggcgccgc cgcacacgaa cagtatattg ctcgtattga cttgaataaa ctcttgatga
    33001 ggatgtttgc gtccgccttg cggcggcacg ttcgcgacgg tgccctcgat cagtttcagc
    33061 aaggcctgtt ggactccttc gccggatacg tcgcgcgtaa tcgaaggact gtcgctcttc
    33121 ctgctgatct tgtcgatttc atcgatgtac acgatccccc gctccgcacg ttcgacgtcg
    33181 tagtcggagg cctggaggag cttcaggatg atattttcaa cgtcctcccc gacgtatccc
    33241 gcttcggtga gcgtcgtcgc atcggcaagc gtgaagggca catccaagta cttcgccaag
    33301 gtttgagcga gcaacgtctt gccggttccc gtcgggccca ccatcaggat gttgcccttt
    33361 tggagttcga catcgtcgac gtctttttcc ttggccgaaa tgcgcttgta atgattatgg
    33421 accgcaaccg agaggatttt cttggctcgc tcctgcccta cgacgtactg atcaaggtga
    33481 tgcttgattt cagacggctt cttcagtttc gacgaaattt cttccttcgc ctcttcccaa
    33541 tcctccgcaa tgatgtcatt gcacaggttg acacattcgt cgcagatgta gacggtaggt
    33601 ccagcaatca gtttacggac ttcgtcccgg cttttgccgc agaacgaaca gcgaaggtgt
    33661 cgatccatct tttcctgctt ggccatgcgt cctcccgtgt tacttgtctt tggccccgtc
    33721 cttctccgac gccaccgctt tcaatgcttt gggtgctcgc gtaatcactt catcgatcaa
    33781 gccgtaccgc ttggcctctt cgcccgacat aaagtagtcc cgttcggtgt ccagcgcaat
    33841 tttttcgatg ggctgcccgg tatgcttggc cataatttcg ttcagccgct cgcggatctt
    33901 cagtatctcg cgtgcatgaa tgtcgatctc cgtcgcttgg ccctgaaacc cgcccaaggg
    33961 ctgatggatc atcacccggg cattcggaag ggcgaagcgt ttgcctttgg tgcccgccgt
    34021 cagcagcaac gcccccatgc tggcagcctg gcccaagcag atcgtgttga tgggcggctt
    34081 cacatactgc atcgtgtcgt agatgcccag accggccgtg acgctgccac ccggcgagtt
    34141 gacatacaga ttgatgtctt tttcaggatc ttccgcttcg agaaacagca gctgagcgat
    34201 gatgaggttg gcgaagatat cgtcgatcgg agcgcccagg aaaatgatgc ggtctttcag
    34261 cagtcgagaa tagatatcgt aggcccgttc tccccgattg gtttgctcaa tgacgatggg
    34321 aactaacatc cagattcttc ctttccgcga aaatgacagt gagacaggac agctatccct
    34381 gaatcaccgc gtttcgatac acgaaatcca aagacttttc tgccaggatg cgtgcccgca
    34441 attcttcgat agaatcctgg ccgcccgcct ggatcatctt gacgagttcc gagtgggcga
    34501 ttttcaattc cgacgctaga cgctggactt cgttattgag gtcttcctgc gtcactgtga
    34561 gctgttcctt ttcagcgatg gcctcgagaa tcaatccgac cttcacacgg cgggccgcct
    34621 catcgcgatt ctcctgtcgc agcttcgtca agtcttctgt ttccagcgcc gacggggcat
    34681 ccgctcccgc ccgacggtgg cgttgcatgt gttgacgaat gatcgcttcc agttcccgct
    34741 ccacgagagt ctccggaagg tcgaagtgat gcgtctcgac gaggcgcttc acgatggtgt
    34801 ccttgtaggt atcttcgact tcccgcttga gcgccttttc catctgcgtt cgcaagcgct
    34861 cttgaagttc ggccagcgag gcgtaggcgc cgcaatcttt ggcaaactcg tcgtccaagt
    34921 ctggaagcgt cttgatcttg acggcgttaa tgttcactgt gaaggtgaca gtcttgcccg
    34981 ccacccgaga atccggatgg cttgcaggat agggctgcga aatttctacc gtatcgccct
    35041 ccgcttttcc gagaacatgc cggtcgattt ccaaccctaa cacggtcgcg tttgaaccga
    35101 ccttgtgaag atggcccgct ttcttactgc cctcgagcgg ctcattgtcc aggaaacctt
    35161 ccagcgtgag gatgacgaaa tccccttcca cgaccctgtg ccccgcggga ggtgcttcca
    35221 gcctcgcatg ctgctcgcga aggacgttca tgcctttggc cagctgttcg tcggtgacgg
    35281 ttctggtgtc aggcttaagc gagatgggat tcggcgcctt atagtcccgc aattcgattt
    35341 ttggcttgat ttcgaccgtc gcagtaaaag tgaatggtgc atctcttttg attttgacac
    35401 gctcgagcgg aggaatctcg acgacaaccg gagagatacc ggcctgtcga acggcacggt
    35461 cgtaaaaatc cggcaccagg ctccgaacga cgtcctcctc cactgctttg gcatagcgct
    35521 gctctagcaa cgcttgcggc gctttgcccg gtcgaaaacc gggcacgcga acctgccggt
    35581 tcaactcctt gtatgccaac acaaagcgct gcgcgacctc ttcaaccggc acctcaattt
    35641 tgagggcccg tttcatggga ccgagttccg tgacttccat tttcatctgt cgagcgattt
    35701 cctttgcagt ttatggcatg cccgctgccg gatggtgcga gaggggggac ttgaaccccc
    35761 acggttgccc acgagatcct aagtctcgcg cgtctgccag ttccgccact ctcgcacggt
    35821 ggggaccgcc ggatggggtg tccagcctgt agcaaactag cgcaactaca cggcgatgct
    35881 gctattttgt accgttgcat gtgtccaact gtcaacccat actccagcgg ggatcgaaga
    35941 gaatttgccg atttttgtct cctgaggggt aacaggtggc acggccagtg cacacaccaa
    36001 gattctgaaa ggtggctcag ggtgtggtgg accagtataa tggaccatca tgtgtggtct
    36061 catctagtgt cagtacaagc agggaggtgc ctcatgcgca tcggcttgct gctcatcagt
    36121 ctcactctcc tcgcgctgac cattgattct tctcccgtcc tggcatatcg ggattacctc
    36181 accgccgaac aaaaggcgca actcgagaag attcataccg tgcttgtcga ggcgattgcc
    36241 ctcacagaca aggggactgt tgatgccggt ccgatcaccg aagttgccgc ccgtcggttg
    36301 ggggaaatgg gctacgccgt ggtgcgagat gcgggcaaac cacacgatgc gaccttcaag
    36361 gtgaaatgcg agcagcgcaa aacctgggaa ggtacgaccg ctgcgggcgg cgatgcagat
    36421 ctcccggatg ccccctcgag gctgtggaaa gggcctgcct gccagttgag ttatctcctc
    36481 ggagagacga agattaagtg gcaaaaggaa gtgcggactg agttcgaaga cgccgcagcg
    36541 gcggctcaat ccgcgaatgc cggcgatccc ggagcctttg cgatgagtca attgcgcgcc
    36601 aagctcgaac agtacgattt ccccgtcctc ttggctgccg agtggggtca tcccgatcga
    36661 ctcctgaagc tactcgacgc ctctgacacg ccgcaggtgc gcaagatcaa aattatttca
    36721 ctgctgggcg aaatgcaggc ggacgaggcg ttaccccaac tcaaggcagc actcaaagac
    36781 aagaatcttt ccaaggaggc ggcggtcgct ttgggcagca tgggcaaaga aggcattccc
    36841 atactcgtcg acattctcaa gcactccaaa cagccggaac tccaggccgc ggccgcaaaa
    36901 ggtctggggg acttggggaa cgtccataac gactcgtcgg tcgtgcctcc gctcttggaa
    36961 atgctggatg caccgggtgt tgatatcact gttcaaaccg aggtcgcctg ggcgttagga
    37021 agagttcccg accgccgctc cgtgaagccg ttgtttgatc tggacaagaa actgcaaaaa
    37081 atccggaagg acccgccgga cccccagatc aagaaactca aggaagcggt gttctggtcc
    37141 atcaaacagg tctatacgga agaccaatac agctagacgc gaaaaccatt caactccggc
    37201 cctttgtttt ctctccgcgc tgtgcctgct cttgctggtt cgctgatcca aaggaatccg
    37261 tgtcggttcc agcggcgacg cttgagacaa gggtggaaac tatgaaatca tgggtccgat
    37321 agtcttacga acatcacccg agggaggccg gcatcatgaa tgatatgtca cgcaggcgct
    37381 tcttacagtg gacggcggca accgggggga cactcgcgct cggaggcctg atgaaccgcg
    37441 gcgcgctact ggagttcggc gtgcggatag cgcatgccga tgagccgatc aagatcggta
    37501 ttctggatcc cttgtcgagt ccttataaaa cctcctccat ccacgacgtt catggggcca
    37561 acgtggcggt cgatgtgttc aacaagaaag gcggtgtgct gggacgcccg gtggccattc
    37621 tcgaggcaga cgatgcgtcc aatcccgatc aggccgtcaa agtggcgacg aagtttatca
    37681 aggacgaccg cgtcgatgtg ttgatgggca cgtttaacgg cgactgcgcg cttgcggtca
    37741 gtgcgttggc ggccaaggaa aacaagctct tcatggtgac ggctgctcac atccccgaat
    37801 tgaccggagc ggcctgcaat tcccatacgt tcgtctttat gccccatgct cgcatgctgg
    37861 cccacgcggt tgcgcctcat ttagcaaagg cgtacgggac ccgctggttc atgattaccg
    37921 cgtcgaccat cgatggaaaa tcctgtgctc aggcgctcgt cgatgcctcg caggcgcaca
    37981 aggtcgaaat tgtcggagag acggttacgc ctttcgggac gaccgatttc gggccggcat
    38041 tcaccgccgc aaaagagaag cagccgactg caataatttt gaatctctat ggctgggatc
    38101 tcgtcaatgc gctcaagagc tatgcgaaac tggagttggc caaggagaag atcggtatcg
    38161 gaggactcat cagcggcgaa cagatcggac gcccgctggg ctatgccggc aatgcaggga
    38221 tctgggggct gatctgggac ccgaagatca atacggaaag ttccaagcgt ttcatccagg
    38281 gcgtgatcga caagtacaat cacacgccga cgtcccgctg ctatctgggc tatgccgcga
    38341 tgacgcaaat cctggaagcc atccagcgag cgggcagcac ggatacgcag gccttgatca
    38401 agactctcga aggccatgag ttcgacgggt taaaagaagg caagtcggta tttcgtgcct
    38461 cagatcatca acacgtccag gatgtgctcg tcggggaagc gtatgggaag gagctggggt
    38521 tgggtcatta taagattctc tcgacgattt cggggccggc caaccccggc gccgccgatc
    38581 aacagctgtg caaacactaa tgctgtccgg cgccgggagc gcaacgatgc gcccccggcg
    38641 accagccagc cgcttacctg gtcacgcgaa cttcgactcg tcggtttttt gcccgtccgg
    38701 gttcggtcgt gtttggcacg attggtcgag tatcggcata gccgtgcgtc gtcacagcag
    38761 tcaaaccgcc ttctcccaac gccatagcag cattggtggc ccgggcctct gaaagctcct
    38821 tgtttgaggg gaatttcttc ctgagctcgt ttctaatcgg ccgattgtcg gtgtgaccgt
    38881 caacgctgac tttgtattct ggaaaatcct tcagaatcgc tcccacgtgt ttgagggcgt
    38941 cggagccggc ggatttcagc tgatgttcac cggatccgaa cagagataat aaaaaaacgg
    39001 gcgcatccat tacttcatac cgaagacgtg caatgcgcac acttggtggc tttgagtgga
    39061 atcgtcgaca ggcacaatgg gcaatctttc gtggtcgcag ctgcgggtgc cgtcggcttc
    39121 ttcagccggt tgatttgttt gaccagcatg aagatgacga atgcgatgat gatgaaatcg
    39181 aacaccgttt gcaggaacac cccgtaattg atcgtcggag tccccgctgc cttggccgcg
    39241 gcgagcgagg gctgaggcgt gctcgacaga ttgagaaaga ggctggagaa gtcgacttta
    39301 cccatcagga ggccgattgg cggcatcagg acatcgctga cgagtgaggt gacgattttc
    39361 ccgaaggcgg cgccgatgat cacaccgatc gccatatcga ggacgttgcc cttcatggcg
    39421 aactccttaa actctttcag catagctccc tccagtgagt aagaaggtga acggcaattg
    39481 cctagcgttt tctggtcgtg tccagcgcgt aaccgagtgt gatgagatag aggttgtcgg
    39541 tgtcgccggt gcccggcgcc gggcgactat tgtagcgagt ggtgatctgg aagctgctga
    39601 tgaacccttg ccagatcttc aagcggatgc cgttatccat cgtcaggaac acatcggacg
    39661 tattttggag gctgggaaac aattcgctga ggtgatagag cgtgaagcgg tcttcgaaga
    39721 acggccaatt ccacttgacc gagatacgcg cgcggacgct cgacttgtct cctgcgacgt
    39781 tgaaatcttc gttgaaatac gccaaaccgg cttccgtgta gagcgtcatg tccttgccaa
    39841 tccctgagaa gtcttcgcgg tcaatccact gatagccggg accgctggcg agggcggttc
    39901 tcaatttaag gtcctggaac tgatcgtttt caaagtaggc cgacgcgaac cagaagaatc
    39961 gctttgtgat gaaaaagtcc agcttgatgg tggcccgggc atttcgcgtg atgatcgcgc
    40021 ctgagtcatc gccgtacaca taacgaacgt tcatggtgag tctgagcagt tcgcttcgcg
    40081 cgaccaattc gccgagcaga ctggcgttgc gcaaatggct gttccccgtc gtttgcgaat
    40141 agccgcctgt gaggtttcca atataaatga cagggggttg aatcagggga ttgagagacg
    40201 agacgtcgct catggggatg tccatcgtgc ctttgagcgg ctctgcgtga atgacgagca
    40261 ctccatcggg tcccggcgta gcagtaccgt tgaggatcgt gccttccttt aaatgaaatg
    40321 gaattgggtg cgtgatgttc agctttgaca cgtcttccca tttgagcttt atcaggttgt
    40381 cggagactga aggatttttt agaaagagga tcccaccggc catttcgagt acttcaccgt
    40441 agatgacgct gccgtccttc agcgtgacca cgtcgaccgg tgcgggagcc ggcggcgcgg
    40501 gttcctcagc agcggcctgt gtcgtcggcc caccaatgag tatgagcaag agaactatac
    40561 cgaccagaac actgctcccc atcatccctc ctcataatca aagcggttgt ggcacagatc
    40621 tcagagatcg cgcccgtgac cgacgcgctt agggggaacc gtataaacga atagtcagag
    40681 gttggcaacg aaataagata agccgtgcgc gaacggaaga cgctgcagcg gcggcaacgg
    40741 gaatagccga catgaaagac ttagtgaatt accggagcga ccagcagggt gatatcgttg
    40801 caaggacaga tggtctacag tgcagatgct gacgaataaa ggtgagcgga ccgatggaag
    40861 gcagcgctat cgtccgaaca ggccttttaa aagctctttc ccttctttct tgaggtcggc
    40921 tggtttggtc gtgccttgca tgagaccttc gacggtctct ttgacctttt cctgaacctg
    40981 ttcctggact tttcccgtca gtcctcgggc atccagtgca aatgaaggtt tttgcagagt
    41041 cccggtgatg agcaagggca atcgcagccg gccgccctgc gaggccgcct tcacgagtgc
    41101 ggagccggcg atcttttggg tcaatgtttg agaaagattc aaattcattg cgagattgag
    41161 cgtttgatca aagccgactg tcccatgacc tgtcgcctga aagtcgtgac tgtccataag
    41221 gattttttgc gcattcacac taccgtgttt gatcgtgaag tcgctttcga tcgtcgagaa
    41281 cgccgtcgcc ttggcttgct caagcgaaat accggcgacc ttcagcaatg caacggcttc
    41341 cgccatgagg ttcacgccct cgatttttcc gtctttgatt tccacatgcc cgggtccctc
    41401 cagcgctctc atcagatcat cccaggaaaa cccgcgtccc gcgacggcca cgtccgccgc
    41461 agctgttccg ctaacctgga gcgggctctg cggatcgacc gcggccagtg cctgtccgat
    41521 ttgcacacct tgcaaggtca gcttgccgtt gaagggcggc gtggaagtcc cgagcgacgt
    41581 gcctccctgt gccttcacct gcccgttgaa gacctcaagt gagaaattgc tgagccggac
    41641 ctcctggccc ttcagatcag cgttcagctc cacatttctt agttcgacgg gtttcttgag
    41701 gccgctctcc accggaagat cggccgtatc cacggacggt gacgagacca tcaccctggc
    41761 gtggccccca atggcgttgc ctctgacagc gagcgaagat ttgcccatga ccaccgcgag
    41821 agtcaggtct gtgacgtcgg cgagtgccag tggcgaggcg cctgacttga gcgggtacgg
    41881 cgctttggca acaagacgaa ggtctttgat ttcgaccggc ctggttaaag ggagcgcgac
    41941 ggggacatca gccgtgctga tagagggagc ggttacgacc gcattcaatt gccctccaag
    42001 caatgcgcca tgcaatgcga caaccattct tccgactgcg agagtaaact ggtactgctt
    42061 gatctcaagc gcctgaacca gcgggccaaa gctgccgtcc agtgtgacag gaagattgtg
    42121 cgggagagtg gtcgcgctga agtgaatggt cggcgtctca cctagatgaa ccgacttcag
    42181 ggccagcacg aaatcttgaa ttcgatattc cgtttccggc gctgttgaga ggtcgcgata
    42241 gaccaccgtg cctccgtcga tcgacagacg gtcgacagcc agaagtgcga gggcttccag
    42301 cggatcgccc tgctgttgcg gagactcagg ctgcctgcgg tcggcaggaa caggtgtttt
    42361 gggtccaatc gtcgagacgt tcgtgacgcc ggccttgttc ttgatcacgg tgatcacggg
    42421 atcgcgcaag gtgatttctt cgacttcaat tctctttgtg aggagcggcc acaacttcac
    42481 gccgacatct agcgagctca acgaagcgaa cgggttcgca gtgaaagacg gatcgtccat
    42541 gacggtgaag ccggtaatcc gagccccgat gcggggccaa atcgtcagtc tgatgccctg
    42601 cacgtggatc ttgcggttca acgcttcttc gatgagaggc ttgtactggt cttgataggc
    42661 tgcgagatcg acaagaaacg gaagggacag taaggcaacg atcagtaggg ccaggacgat
    42721 gactatcact gaaagaatct tcatgccagg tctcctcgca ggtcggcgca gtatagaaaa
    42781 gctgggtcac ccggtcaaac gcgccttgcc gctcattaga gtgcatgcta aacttccttt
    42841 ttcgattcgt tgacggagag gtgcgagagt ggacgaatcg acatgcttgg aaagcatgcg
    42901 tcccggcaac gggaccgtgg gttcgaatcc caccctctcc gccataaaga attcgtgaga
    42961 gctgggaatc atgttccgag cggaaaggcc ttccgctata taagtcaaaa agagagggtg
    43021 ggattcgaac aggggttctg aaggggatat gtccccttca tggggagcgt acgagggggc
    43081 cggccccctc agtaggagtg tgagcggtta gggaacgcga cgtcgaatgc ccaccctctc
    43141 cgccattctt ggcgcgacga aagctgccgg attgtcgacc gaagggcagt gacgcgccag
    43201 ccttaccaga gggcaatgaa gttttctgcc catgaaaagg tatcgctccg cgataatgtt
    43261 ttaaaattag gaataggcct caaccttgtt cgggccacaa cggcgtggcc ccgtggggtg
    43321 tgtggcgagt atgggacgtc ggaatgccgg cgcaccaaag acgagccaga ttaggggctg
    43381 ggccctgtgc aacagaaccc tgtgaacccc gccaggtccg gaaggaagca acggtaagca
    43441 gaatgttttg ggtgccgcag gatcacctgg ccccgctttt ttaattatga tgttgaaata
    43501 ggcattccgc acggtctcgg gccttcacca tcctcgacgt gcaggacact gtatgctcat
    43561 atgtgttaga tagcctcttt ccacatcctg ccatgtgaaa gaagaacacc agttatggac
    43621 taccaagttt ccgccagaaa ataccggcca ggtacctttg acgatgtgat cggccagccg
    43681 catgttgtgc agacgcttgt caacgccgtc accagcaagc ggatcgctca agcctatttg
    43741 ttttcgggga cgcgcggcgt gggcaagacc accgtcgcac gcatccttgc caaggcgctg
    43801 aattgtgagc aaggacccac cggaactccc tgcacgacct gcgtcaattg caccgagatc
    43861 gcgcagggtc attccgtgga cgtgatcgag atcgacggtg cgtcgaatac gagcgtcgat
    43921 gatgtccgtg agattcgcga aaacgtcaaa ttcgcaccgt ttcacggcac ctatcgtgtc
    43981 tatatcattg acgaagtcca catgctgtca aactcggcct ttaacgccct gctcaagacg
    44041 ctggaagagc cgcctcctca cgtcgtgttc attttcgcca ccacggaaat ccacaagatc
    44101 cccgcgacga ttctctcccg ctgccagcat tacaattttc gccgcattgc caagacggag
    44161 atcatcgaac ggctacgcca tgtagccggc caggacggca ttgcgctgga ggaccgaagt
    44221 ttggctgcgc tggcccgagc gagcgaaggg agtatgaggg atgggttgag cctgctggat
    44281 caagccgtgg cctttggcgg caagtcaatt gtgcacaccg atctcgaagc tttgctgggc
    44341 gccattccac aggaattggt tcgatccttg attcgggcga tcacttccca ggacagcgcg
    44401 ggggccctgc ggactttgtc tcaactggta gaaggggggc acgagctgcg tgcatactgc
    44461 gctgatgtgg tggagtatgt gcgtcatctg ctggtcgtgt ccgtcacgcc cccgcaagaa
    44521 tggcccgcat tgatagaggt ctcctcagac gatcttgcgc agctcgcgac ggacaaggcg
    44581 tcgctgaccc cggaacagat tcagcagatc tttacgttgt ttctcgaggc ggaggacgcc
    44641 ctgcggttca gcgctcatcc tagatttgtg ctggaaacgg ccgccataaa ggcgacccgg
    44701 ttgctaggcc ctcaaaccac aatcagtgac cagcccgggc gtgcagtctc tgcttctggt
    44761 gctccgcatc catctgctcc ccggtctgat aagggatcga gcgcaccaat tggcggctcc
    44821 tcccaacctg cacgtgcacc tcaaccccag cgcgctgtga ctggaacctc ctcctcatct
    44881 tcttctccag gctccggagt tccggcagcg acatccatgc cgcgcgccga taaaacgccg
    44941 gctactcaac cgatgaaaca ggtggggcag tcctcctcct cggctgcgcc ggcgcgtcct
    45001 ccgcaagcga tcatgctgaa ctgggagcag tttcaagaag aggtcggcca tgcgttccca
    45061 aatattgcgc cctttctcga aatggggcgc ctggttggga ttgagggcaa tgtcgtgaca
    45121 atcggattcg cgaagcaggc gacagtggca cgcgggatgt tggagaaggc ggacaatatt
    45181 caagcgttgg cctcgctatg cgagcgtatg gggggccagc cggtccggct tcgtgtcatt
    45241 gagctgacgg aagcggacgc tccgggtcct accatggccg agatcagagc cgcgaaagag
    45301 caggaacaaa aagtcgtact ctttgaacgg gcgaaggccc atccgctcgt caagcaggcc
    45361 ttggaaatgt tcggggccga acttgcggat gtcaggacga tcgccaacca acaagaggtg
    45421 caggaatgaa aaatcctctc ggcaacatgg cgaacctcat gaaacaggcg cagcagatgc
    45481 aggcgcaaat ggctaagctc caggaggaag ctgcgaccaa aacggtgacc ggctcggcag
    45541 gaggcggcat ggttacagtg accgcgaacg gcgcgctgga ggttgtcagc gtccgtatcg
    45601 atcctgaagc cctgaaagac gcagatgcgg agatgctgca ggatgtcgtg ctggcggcca
    45661 cgaatgaggc cctaaagaac gcgcgtcagc tgatggccga gcagatgaaa tcggtaacgg
    45721 gcggcatgaa tattcccggc ttgttctaac gagtcgccga tggctgttga tcaacaagga
    45781 ttgctgtcac gcctggttcg agagctggtt cgtcttcccg gcatcggcca aaagaccgcc
    45841 cagcgcttgg ccttccatat cctcaaggcc gaccgagacg atgcgctgcg attggcggac
    45901 gccattcgcg ccgtcaaaga cggcctctcg ttctgccgtc aatgccggaa cattgccgaa
    45961 ggcgagctgt gtgagtattg cgtggatccc aagcgcgacc aaaccaggat cctcgtggtc
    46021 gaggaaccga gtacgctgta tgccgtcgag cgagccggcg cctaccgagg gctctaccac
    46081 gtcttactcg gcgcgctttc tcctctcgat ggagtcggac cctcggacat ccgagccgaa
    46141 gaactggtcg accgggtcaa actcggaggc attcaggaag tcatcttggc aacgaatccc
    46201 acgatcgagg gggaagccac ggccatttac ctgacgaatc agttgaagcc gctcggcacg
    46261 cgtgtgtcgc gtatcgccta tgggatcccg gtcggcatgg acatcgaata tgcggatgaa
    46321 gtgacactgc tgaaatcaat cgaaggccgg cgcgatctct agcagcattg tcgccgattc
    46381 atgctttccg gctccatgca gaatgaaacc cgcctttccc attgaccgcc tttcgaagcc
    46441 cctgttatag tttcaccctc atttttcgtc atgctattct tcattggtga accatgaaaa
    46501 cgaaggcgag cacccgcaga aatcctgtcg ccgtgaagcg aggcagcaaa gcagcgtcag
    46561 ccggcggaaa atacggcgca atcaggcgtg agctgcagaa acagcggcaa gccatcctgg
    46621 atgaaacggg tgaagggctg acgaaacgcg aagggccgga ggcatttccc gacgtcagtg
    46681 atcaagcgtc cgccgaagtg gatcagaatt tctctatgcg catcaaagaa cgggagcaga
    46741 agctcctcaa gaagatcgat gaagcgcttg accggttgag caagaacatc tatgggattt
    46801 gtgaacgctg tgaagaggag atcccctatc agcgtctcaa ggccaggccc gtgaccacgc
    46861 tctgtatcgc gtgcaagact cttcaggaac aggaagagaa aatccgccgg tagtctctcg
    46921 cccttcttct gcccgtccta ttgaggtttg agcttcttca ctcgttgctg gaccaacgca
    46981 atcctgtcgg cctcatcagg aacaccttcc accaacgcga gatacgtctc gtattcggca
    47041 agcgcgcgtt tcggattggt cggttcgacg gcttccgcca aattaaaacg agcaatcgca
    47101 taattcgggc gaagggtgac cgccttttca aacgaagcga gggcgcccgc cttatcgcct
    47161 aatttgagct gcgttgaccc gagattattc agcgtctccg gcgtattggg cctgatggcc
    47221 aacgactgga gcatttccgc cttggctttc tcgtattggc ctagtccgaa gagagattgg
    47281 ccgaacttgt ggtgtgcttc ggccagccaa actttgttgg tgaatccgct tgcgatggat
    47341 ttccggtacg catcggccgc aatgtcatag ctctgcgttc cgcaataaga cagctgtgcc
    47401 gtctgacccc gtgcgaactg ttgaagcgag gcgaatggct ccttgtcttc agcgccttgg
    47461 ctatccactt gctggccggg ttgaacgccg ctcggctgcc ggagacggtt caaaaactcg
    47521 cgccgatacg gcgagaagag tctgacatag ggatcaatgg tgacggaggc ctggagcagg
    47581 gtgggacgat cgcgtgcgct gatcaccaga tactgggtgg tggttttctc atcacccgtt
    47641 tcatacagcg agctggagtc catggcggac ttgcctgcga agttggcgac gctgagataa
    47701 aattcaagcg ttggtttcaa cagcgtcaag gtatggcgga gcaccgaata aggatcgagg
    47761 tcgagtccag acgaaaatgt gaacagagcg ccgaccagcg tgccatcatc gtcaaaaaaa
    47821 aacgattcgt ctccgtgaga cgcactgcgg ctcgctggta gcatcaattc ctgaccggtg
    47881 ccccagggtt tgatcgtcaa gctggactgc ggatgctgtt gaaggaaatc cgtcttgcgc
    47941 tgacacaatg ttcccggctt gaggaggact aagtcgcttt cagaaggcgg aaggttcggg
    48001 cgtggattag gcgtttcaca ggccgcagcc aggctcacag tgagacagac aacgagcccc
    48061 atcaatgctc gtcctgaagc agagcgtcgc tgtcgaaggt cggtgggcat gatgagactg
    48121 agaataccga aaggcccagc ggtttgccag ggccgaaaat gacaacgccc gctttctcgg
    48181 tcgagaaagc gggcgtgtca gatttttggt gagcagtgag ttagtactga gaggcgcggc
    48241 ggcgctggct atccaacatc gcatcctcgc tggtatagcc gaacaggtac ggcataccgc
    48301 tggtcagcga gtaaatggga cggtttacgc catagtcgag cccttgtccg atgggatgca
    48361 gcaggaatcc caaccagcgc caaggattgt cgttgagcgg cgacccagct gccacgtccg
    48421 tatacacgag cgctgtgctg tccatgacac tgtagatgct ggccggggcg acgtacccga
    48481 tgtctccgtc agccgcttgc tggtggcctg cgcaaccaat caggccggcg atcaaaagca
    48541 gactcactgc aatcaatttg gctttcatcc tctacctcat gtctcgaaat cggttccaat
    48601 gaccaatcta attaactgaa ggaagtgtag ccgaggttca gcaatctgtc cagaaaacga
    48661 caggtttaca ccgacgcagg gtcttgggtg agatgcgagg aattccgatg ggttgagagg
    48721 tggtgggcga tacaggtatc gaacctgtgg cctcttccgt gtgaaggaag cgctctacca
    48781 ctgagctaat cgcccatcgg gcggagtcta gcatgtgccc ttcggctggt tcaatccatc
    48841 cgccggccgc tttgccttga catcgccgaa atacgattcc tacaatgctc ttctgctcga
    48901 tctttctctc gcggagcgtt ccactcagat gcgcgaagac atcgtgatta tcggcggcgg
    48961 tttggccggg tccgaagcgg catggcaggc ggctcatcga ggcgccaaag tcacgctcta
    49021 cgaaatgcgt ccgaaggagt cgacgccggc ccataagacc ggagggctgg ctgaactcgt
    49081 ctgttcaaat tctctcggat ccgccgaccc tcagaatgcc gcgggcatcc tcaaggaaga
    49141 aatgcgccgc caccagtcat tgatcatcaa ggtggcggat caggtgcgtg ttcctgccgg
    49201 gtcagccctg gctgtcgatc gggaccaatt ctccttcttg atcacgcagg cgctcgacag
    49261 tcatccgaac gtccgcatac ttcgggaaga aatgaccgaa atcccgaccg actgtgtctg
    49321 catcgtggca acagggccgc tgacttccga caaactgtca caagccattg cacggctcac
    49381 gcactccaac catctctatt tctatgatgc catttcgccc atcgtggatg cggactcgat
    49441 caatatggat attgccttcc gtgcatcgcg ctatggaaaa ggcggtgacg actatctcaa
    49501 ttgtcccatg gacgaggcga cgtatcaggc cttttatgac gccttgctgg cagcggaaaa
    49561 agtccaaccc aaggcattcg agcagacgcc gtatttcgag gcctgtttgc ccatcgaggt
    49621 gatggctgag cgtggaaggc aaaccatgca gttcggtcct ttgaagcccg tcggtcttga
    49681 ggatccaaaa gccgggcgcc gaccctatgc cgttgtgcag ctcagaacgg aaaacgccca
    49741 ccggtcgtgt tacaacatgg tcggattcca gacgagattg acctatggcg agcagaagcg
    49801 ggtttttcgc atgatccctg gcctcgcaca agcggaattc ctgcggtacg gcagcctgca
    49861 ccgcaacacg ttcataaact cgccgcagct gttgcacaac acgctgcagt tcaaagagcg
    49921 gccgacgctc tttttcgccg gccaactggt cggtgtggaa ggatacaccg actcagccgc
    49981 gatgggaggt ctggcgggga tcaacgctgt ccgtgagctc gacgggttgc cgccgctcac
    50041 cccgcctccg acgacagcac atggctgcct gctttcgtac atcaccgcga gcgaccctcg
    50101 gcatttccag cccatgaaca tgaatttcgg cctgtttccg cctctcacgg tgcccgtcaa
    50161 agataaagag cgcaagagac agctcatttc gcagcgcgcg ctcgaggatc atgcggcatg
    50221 gatgagtcga tcagggcttt cataacgttt ctcgagagtg agcagcgggc ctcgccggaa
    50281 acggttcgga attacgcctc ggatcttcgg caattagaca ggtttctcgt ctccagccgg
    50341 ctggtgtcat ccccggtcga tccggcttcg ttgtcctcag atgctgtccg tgcgtatctc
    50401 gaatggcttg acagaaaagg agaaaagcac gcgtcattgg cgagaaaact ggccgcgatc
    50461 cgaagcttct atcgttatct cagccgtcgg ggtctcacga atcgcaatcc cgtcgacgcg
    50521 ttgcggacac ccaaacaagc acggtctctt ccacgtgtct tgaccaaaga tgatgccgac
    50581 gccctcatgg cctttcccgc cggcgaaacg gcgggatcgt tgcgtgaccg tgcgttgctc
    50641 gaaacgctct attcgacggg agcccgcgtg agcgagctgg tctcgctcaa tgtgggcgat
    50701 gttcatggct ccgaaggcct tctccgactt cgggggaagg gaaggaaaga gcggattgtg
    50761 ccgatcggtg aactggcgtt ggaggccatc acacggtatc gagccagtct tcccacatcg
    50821 ctgcagagag caggccatac ttgtccgctc ttctacaatc ttcgtggagg ccggttgacc
    50881 agccggagcg tcgcgcgaat cgtcgctcgt tattcaagcc gcttgtcagg cgggccggtg
    50941 agtccacaca ccctgcgcca ttcctttgcg acgcacctgc ttgacgaagg tgcagatttg
    51001 cgcgcgatcc aggaaatgct ggggcacgct tcgctcagta cgactcaacg ctatacacat
    51061 gtcgcgacgg atcagttgct ggccgtttac gaccgcgctc atccacgatc tggtcggacg
    51121 tccaacggcc gaacctcaaa aaacggctcg tcttgagatc ggcgggggaa tttgtctggg
    51181 cctgagcgcc tgaacgtgat atggtcgcgc ctgaccgcag attgatcatc gtgtgaggaa
    51241 ccgacgatga acaaactcat ccggaaagcc aacgtcctta tcgaagcgtt gccctacatc
    51301 cgtacatttc gcggtaagac catcgtgatc aagtacggcg gcaatgccat gacggaatct
    51361 tctttgaagg agcgcttcgc ccaggacatc gtcctcctga aatacgtcgg gatcaatccg
    51421 gtcatcatcc acgggggagg gccacagatc gacgagatgc tcgaccgcct cgggatcgag
    51481 tcaaaatttc ttcacggcgt gcgggtcacg aacgaagcca ccatggaaat tgtcgaaatg
    51541 gtgttggccg ggaagatcaa catggagatc acggacctca ttaatcgtca tggcggcagc
    51601 gcggtcggtc tcagcgggaa ggatggagga ttgatactga gcaagccgtt gaccgcgaga
    51661 gcctgggcgg aaagtcttga gcgtgatctc gaggaagagg acgcggaaag tgatttcggg
    51721 tttgtcggcg acatcgaaaa agtcgatccc agtctcctct tcaagctgca aggcgaccac
    51781 tatatcccgg tcattgctcc aatcgggacg gaccgcgaag ggaataccta taacatcaat
    51841 gcggacctgg tcgccggagc ggtggccggc gcgttacgcg cggaaaaact cgtcatgatg
    51901 acggacatca agggtatccg cgatgccaaa agccgccatt tgtccaccgt ctcgcgcaag
    51961 gacgttcaac ggatggtcaa aaaaggcacc atcaccgaag gcatgctgcc taaagtgcac
    52021 gcctgtctgg atgcgctcga gcaaggcgtc gggaaagcgc acatcatcga tggccgcatg
    52081 ccgcatgcca tccttctgga agtcttcacg cggaagggca tcggcactga gatcactgcc
    52141 taactcgtcc cccgtcgaac gtgtcctcac ggcagaccag tatcctccgc cacatgaaag
    52201 cgctggtcgg agaacgacac ccgcacagcg cgcctgaagc gttggcccga gccgcagcat
    52261 acgtggcggc acagtttgcc gggtttcaat ggacgacaac ccggcaatcc tttcgcgcgt
    52321 tggggaaatc ttacagcaat attctcgctg tcaaagctcc ggctggacga ccggcgaagg
    52381 agcgacaggc tccgctgctc atcggtgcgc attacgatac ggtcatggcg tccccgggcg
    52441 ccgatgacaa tgccagtgcg ttggccgtcc tcctcgaagt ggccgatcaa ctgagataca
    52501 cgggcgtcac gcgtcccgtg tggcttgccg ctttttgcct cgaggaacag gggttgctgg
    52561 gtagccgagc cttcgcgctg cacctgaagc aagccgggca gccactttac ggcgcaatcg
    52621 tgctggaatg cgtgggctat gtcagtcatg aggcgggctc acaaggcacg cctcccggtg
    52681 taccgatctc cgtgccgacg gtgggaaatt ttctcggcat cattgggaat gaggcctcga
    52741 ggcatttgat tgcggccgtg gaacggagtg cccgcagcgc cgctccggcc atgccaaccc
    52801 tggccttggc tgttcccggg cgtggcgaag cccttccgga tgtgcgccgg agcgatcacg
    52861 cggcgttctg ggatgagggg tatccagccg tcatgctcac cgacacggcg aacttccgca
    52921 atcctcacta ccatctgccc agcgacacgc tcgacacgct ggatctcggc ttcatcgaag
    52981 cggtgagccg cacggttgcc gcatccgtcg ttgagctggc cggaaaggcg actcggtgac
    53041 aggggcccgt gtgagtgcag tctcgcgccg cgccatagcc cgagtcttcg atcacctcga
    53101 ctacgccgca ctcggtaagg tgtactgtta tgaaggagga agggaatttt ggagggccaa
    53161 acgagagcct tgccgccgtc taggcagcca gatcggcacc gccctgctcg cccggttgaa
    53221 gcccggaggc cggagcctgt atgtcggggc cggagtggca gagttgccgg ccatggtgat
    53281 tgaatcactc gatttgtacc ggtcggtgtt gccctataac cttcgtcggc gggaggtcgc
    53341 gttgctgaac cgcgcctgtc gggccctacc tctgcggttt tatgcggtgg atgcggctcg
    53401 agcgtccgga cccttcgacc atctgtggat cgtgagcgtc ttgaacgacc cggagcggtt
    53461 cccgcagcta gcggcgttgt cgtatggccg ggcggtgccc gtgacctttg atgcc
//
LOCUS       DASSCQ010000041        11626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_40416, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000041 DASSCQ010000000
VERSION     DASSCQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40416"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1103
                     /locus_tag="VFH05_02925"
     CDS             420..1103
                     /locus_tag="VFH05_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HET6677193.1"
                     /translation="MPVHWTSILISFLIITSGVLGSQTLIRVDQDLRVIYAEYTLAAT
                     DLGHVNGELIRYRTSVLRAIEAGTKEEFQRIEASLQHKQERLDRAMKRFIDATNEASS
                     GKRMDARELAEVKAVRERMDAYIASSDQAIKMMKQRWSVDANAEAQRLQNTAKQYWAN
                     DSTIKFNSVTVELDRLLEVVAEIAGEVKKEADASLRIATVALIAVSLTLALVTLVFPL
                     RPMRIRNRA"
     gene            1300..2238
                     /gene="lpxC"
                     /locus_tag="VFH05_02930"
     CDS             1300..2238
                     /gene="lpxC"
                     /locus_tag="VFH05_02930"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET6677194.1"
                     /translation="MRLQQTVASSASCKGVGLHTGKPVMLTIRPAPADTGVVFVNRNG
                     HAGASLAASVDHLVATELCTAISGNGFQVKTIEHVLAALAGLNVDNAYVDIDASEAPV
                     MDGSSAPFVRLIESAGVVSQNRLRSFLKITRPLEIVDGSRRIRIEPASTPRITYTIHY
                     DHPLIQTQTYAYEHSSRAFASDIAGARTFGFLQEVEALWARGLGRGGNLENTVVLSQD
                     GILNESGLRFTDEFVRHKVLDLIGDFSLLGLPFIGHLIAVRSGHALHTRLVKQILDHP
                     ECWVLLNADHSAIQAEASLPLSTSAPRSSAVALHAS"
     gene            complement(2443..2946)
                     /locus_tag="VFH05_02935"
     CDS             complement(2443..2946)
                     /locus_tag="VFH05_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6677195.1"
                     /translation="MEELTDILVGLEQRISAYRNRLQDLEKKRRRLDDEIATIKKYLE
                     LADTLHRVEADKAKLSSVATVLIADEKGSRILPVEVTDQSREILLGRSKYAGKSVPEA
                     AYQVLREANRAMHAKELVQRLLEGGLHIKGKTPLTSVATSLKRDKRFRKVGPNTFEAV
                     EDVLSHA"
     gene            3080..3892
                     /locus_tag="VFH05_02940"
     CDS             3080..3892
                     /locus_tag="VFH05_02940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET6677196.1"
                     /translation="MQPLDEAVTTARLLSVAVDACKSAGGLLLDYARSGFQIEHKNAI
                     DLVTDADHAAEQCIVDHIRAHFPTHSTLAEERGHDAPPTSRYQWIIDPLDGTTNFAHR
                     FPVYAVSIGVEWNGQGVVGVVYDPTRDELFTAEAGHGAHLNGQPIKVSSTTQLDGALM
                     VTGFAYDIRDTANNNLNHFARFALKSQGVRRTGSAALDLCYVAAGRFDGFWEVKLNPW
                     DMAAGVVILRAAGGRVTDLRGHEHSTFQPELVASNGRIHDEMLSVISESLSS"
     gene            3984..4265
                     /locus_tag="VFH05_02945"
     CDS             3984..4265
                     /locus_tag="VFH05_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677197.1"
                     /translation="MASTIPPSGSSKGNPQSAGAESTSWDVELLRVLVSRNGSQVEAQ
                     WSIHPQLKQDLLPHEWQEITDLMGKVSGIVGTRFSQVLAQADPEPPTNA"
     gene            4311..4643
                     /locus_tag="VFH05_02950"
     CDS             4311..4643
                     /locus_tag="VFH05_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HET6677198.1"
                     /translation="MDTYYHPHDLGKFSDMEKGNKALWDKFMSYYSAVFAEGALTERE
                     KALIALAVAHTVQCPYCIDAYTQSSLEKGSNVEEMTEAIHVACAIRGGASLVHGVQMR
                     NVTEKLSM"
     gene            complement(4671..6536)
                     /locus_tag="VFH05_02955"
     CDS             complement(4671..6536)
                     /locus_tag="VFH05_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HET6677199.1"
                     /translation="MNHTVDSTGKAQVALVNMPFSFSKYPSIQLGTLSSLLKAQGLAV
                     DCHYLNVHFAHKIGVPLYELICEKRALFGEWLFSYLLFRENPKRAEYPRVFKPVFEQM
                     AQQSGRPIGYFEEMATRIAPQFLTWALTAFDWSQYKLIGFTSTFDQNVASLTMAKLIK
                     DLYPDVTIVFGGANYDGEMGLEYFRAFPFIDYVVVGEGEVAFPALVRQVLSTRSDGVP
                     PAVAFRQDGEIRLAPNTALFTDFARTGPPDYDDYYRLLAELGATAQGLDRILLYEGSR
                     GCWWGEKHHCTFCGLNAQSMKFRAKSPEQVASEMTYLSHRYDTARFRLVDNIIDMKYI
                     DQLFGKFAADHCDLDVFIETKSNLHKQQIRTLAAGGVKCMQPGLESLSANQLKAMDKG
                     VTPMQNIVCLKWSFYYRVTVSWNILLGFPGETNEDYLRQIDMIPSLLHLQPPEATGKF
                     WLERFSPYYARPSEYGVRMTGPGSAYEYVYDARQVDLKKIAYDFEYELDNWRVDPHVY
                     QELVSTIERWQRLHATSDRPFLYYSKALGYVTVYDGRDPKAPTRQRYDGVAALVIEMC
                     NEAPKTAEQLLSALAYLDTSQAQLEPILTDLASRRILYQERGRYFTLAIPEHPYL"
     gene            complement(6544..6897)
                     /locus_tag="VFH05_02960"
     CDS             complement(6544..6897)
                     /locus_tag="VFH05_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET6677200.1"
                     /translation="MGLADNKCIPCRGGVPPLPADRIEALLKELGRGWTLNAQGRLER
                     LYTFKDFAQALSFVNRVGAVAEAEGHHPDLYLSWGKCKVEIWTHKINGLTESDFYLAA
                     KADREFEPFRAMAGG"
     gene            complement(6968..7933)
                     /locus_tag="VFH05_02965"
     CDS             complement(6968..7933)
                     /locus_tag="VFH05_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET6677201.1"
                     /translation="MAGTRRRRKPAGQSTALTATELQAAAPHAEVADLHRAIEQDGGK
                     VLSVYREPYGGHWVVLAALPIELVEPTPYQRNLSDTHVRKLEAVISKIARFLDPIICV
                     RVAKENQTTKYWTPNGNHRLSAMRTLGARSIVAIVVPEAAAAYHILALNTEKAHNLRE
                     KALEVIRMYKELMQLPETEEETYALEFEEPALITLGLCYEERPRFSGGAYHPVLKRVD
                     EFLKKPMRTAFESREERARKLLELDDAIVRQVNALKAKGLTSPYLKSFVVARVNPLRF
                     RPKDAPPVSFHEAMERMIQAAAKFNPEKVKVDDLAKSGGMPDESD"
     gene            8026..9369
                     /locus_tag="VFH05_02970"
     CDS             8026..9369
                     /locus_tag="VFH05_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6677202.1"
                     /translation="MRIETIGWFTASCLLVSNIIGGGVFTTTGLMARELGNPMFILLL
                     WAVGGLFAVGGAMVYGELGAQLPHAGGDYVYLREAYGPLVAFLSGWTSFTIGFGAAVA
                     ASAISFSAYAFRIFPVMDESGWWAKGLSLALLWLVTFVHCRGLAGGGRLQRIITSTKI
                     VAIGALIVGGLFASAAQWDWLSVGHPAAIPKAGSIAVAFVIVIYCYLGWNVAGFIAPE
                     ITDPRRTLPRVMIGGTAFVATIYVLLNCVYLSALSVDGLTQEPIVPVAEKSATALWGP
                     SSGQLVAALLCLSIAGAVSAMTWAGPRVYWAMAQDGVLGSWLAQRHPETGVPARAIML
                     QTLWASVLILSGTFEQLIVYSGLVLAFFMCLTLSAIFPLRSRPDRPAGVYRIPGYPIL
                     PAVLVMSSLLVVCSGLVQRPMEAMYGMVTVMAGVPFYVLRKRKSQDDLREAAENK"
     gene            complement(9956..10774)
                     /locus_tag="VFH05_02975"
     CDS             complement(9956..10774)
                     /locus_tag="VFH05_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HET6677203.1"
                     /translation="MKRYRVKPASRLSLKDHDPDDTGDYKKTDEGKAAAKAETEKHIA
                     RVSRLQERLYGNNSRALLIVLQGMDTSGKDSTIKQVMSGVNPQGCKVVAFKTPSTEEL
                     ARDFLWRVHREVPPRGFIGIFNRSHYEDVLITRVHGWVSKTVVKRRYDQIREFEDMLT
                     ENGTAILKFFLHISKDEQKKRLEERIHDPEKQWKWNSGDLEERERWADYMQAFEDVLS
                     ATSTEEAPWYIVPSNRKWYRNLVVADRIAQALEAMNLKTPPPPSGVDFAKLKIV"
     gene            complement(10829..>11626)
                     /locus_tag="VFH05_02980"
     CDS             complement(10829..>11626)
                     /locus_tag="VFH05_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6677204.1"
                     /translation="MPAKPPAKSASASNGVSPQKAPVIMQPLPEIVGKDGAPMVLIPV
                     GEFTMGSDKGDDDEEPVHKVFLDSFYLDKFEVTNGRFAKFVEAIQSEPPWGFADKETP
                     VLRQDQPVRWVNWMEAMGYCLWAGKRLPTEAEWEKAARGSDARVYPWGNDPPTLAHAV
                     FGLKEGSDTVSAIGNRDKGKSPFGVHDLAGNLYEWTTDWYDEQFYSKNPAINPRGPAE
                     GSAKVQRGGSYINGAYRLRSSFRTKGDPTEHDPNVGFRCAQDVPKQP"
BASE COUNT         2490 a         3275 c         3213 g         2648 t
ORIGIN      
        1 aagcggttcg agatgtacaa cgacaccgtg ctgggcttca acaagtcggg caaggaagtg
       61 gcgcgcatcc aggtcgaaga gccgatctat gtcagacccg ccgagcgggt gaactggctc
      121 taagatcggc gccagaggca ccacgttggg agaggctccg gcctctccca acacctggca
      181 gggcttctcc cgatcgcgcg aatgatacgc gttccgggtc gagcaagctc gggttggccg
      241 gacgcatgaa ctgtccgtac aggcgtcaca tgaagtcggg agagcgcctt gtctctccgg
      301 acccatttgg cagtcctgac tccgtgactc ccgcagcact ttatgaagga tagatgaaag
      361 ggcggagttc cagaaaggga ctccgccctt ttccatatca atccagagtg aacgatctca
      421 tgcctgttca ttggacctcc atcctgatca gcttcttgat catcaccagc ggggtcctcg
      481 gatcccaaac attgatccga gtcgaccagg atctacgtgt catctatgcg gaatacacct
      541 tagccgcgac cgacttaggg catgtgaacg gagaacttat ccgctatcgg acatcggtgc
      601 ttcgggcgat tgaggcaggt acgaaagaag aatttcaacg gatcgaggcc tcacttcagc
      661 acaaacagga acggctcgac agggccatga agcgctttat cgatgcgacg aacgaggcct
      721 cttccggcaa gagaatggat gcacgcgagc tggctgaggt gaaagcggtc cgagaacgca
      781 tggatgccta cattgcgtcg tccgatcagg ctatcaagat gatgaaacag cgatggagtg
      841 tcgacgcgaa tgccgaagcc caacgacttc agaatacagc aaaacagtat tgggcaaatg
      901 actcaaccat taaattcaac tctgtcacag ttgaactgga tcggcttctg gaagtggtcg
      961 ctgaaattgc cggcgaagtc aagaaagagg ccgatgcgtc actccgtatt gcaaccgtgg
     1021 ccctcatcgc ggtaagcttg acactcgcgt tggtcacgct cgtctttcct ctgagaccca
     1081 tgcggatccg aaatcgagcg taagacacca ccccattcgc cggcttcttc aagcaaatgt
     1141 gcttatgaaa gtcacacctg tcacatttaa accacaatgc tatcggacaa cggtaaagta
     1201 taaattgtat ataaattcaa ttacttatga ctctcaacgc ctgacaattt tgttggcatt
     1261 agagttgctg aacctccacg gtgaagcgga ggttggtcag tgagactgca acagaccgtc
     1321 gcaagttcag caagctgtaa aggggtaggg cttcataccg gcaagcccgt catgttgacc
     1381 attcgaccag cccctgctga tacgggtgtc gtgttcgtga atcgtaatgg acacgctgga
     1441 gcctcgctgg ccgcgtccgt ggaccatttg gtggctaccg aactttgtac ggctatcagc
     1501 gggaacggat tccaggtcaa aaccattgag cacgtcttag cggcgctcgc aggcttgaac
     1561 gtcgataatg cctatgtcga tatagacgca agcgaagcgc cggtcatgga cggtagttca
     1621 gccccctttg tccgattgat tgaatcggca ggtgtggtgt ctcagaaccg actccgatcc
     1681 tttttgaaga ttacaagacc cctcgagatc gtcgacggat cgcgacgcat ccgaattgaa
     1741 ccagcttcca ccccgcgaat tacctatacg attcattacg accatccctt gattcaaacg
     1801 caaacctacg cgtatgaaca ctcctcccgt gcgttcgcgt ctgatatcgc cggagccagg
     1861 acgtttggat ttttgcaaga agtagaagcg ctgtgggccc gggggttagg tcgcgggggg
     1921 aatctcgaga ataccgtcgt cctttcgcag gacgggattc tcaatgaatc cggccttcgt
     1981 ttcaccgacg aattcgtccg tcacaaggtg ttagatttga ttggcgactt ctcacttcta
     2041 gggctgccct tcattggtca tttgattgcc gtccgatcag gacatgccct gcatacgcgt
     2101 ctcgtgaaac agattctcga ccacccggaa tgttgggtgc ttttgaatgc cgaccattca
     2161 gccattcaag cagaagcgag tctgcccctc tccacatctg ccccgagatc ttcagctgtc
     2221 gcgctgcacg cctcctaaat cgctattccc ctggtgtgtt ctaggtgatt tctacgcagt
     2281 ctgttccggc agatgaaatt gatcacgtcg ggagcgagtg gcctcgcccc cgacgcagaa
     2341 tcgaggcggg agttacttct ttttcttctt cgctgctttc ttcgtcgcca agacgctcac
     2401 ctcccttctc aacactcagg agcctttaca aggccgtcgt ttttacgcat ggctcaacac
     2461 atcttcaacg gcctcgaagg tgttggggcc aactttgcga aagcgcttat cacgctttag
     2521 cgatgtcgcc acagacgtga gcggcgtttt gcccttgatg tgcaaaccgc cctcaagcag
     2581 ccgctgaacg agttccttgg catgcatggc ccggtttgct tcacgcaaca cctgataggc
     2641 cgcttccggt acgctcttcc cggcatattt actccgtcct aaaaggattt cccgtgattg
     2701 atccgtcact tcgacgggaa ggatacggct gcccttctcg tctgcgatga gcactgttgc
     2761 cacgctggac aacttcgctt tgtccgcctc cacccgatgc agggtgtccg ccagttccag
     2821 atattttttg atggtcgcga tctcgtcatc gaggcgacga cgtttctttt cgaggtcttg
     2881 aagtcggttc ctgtaggcgc taatacgctg ttcaaggccg accaaaatat cggtgagttc
     2941 ttccaaggaa ggccccctcg gcaagcatgc ttgctttata tgcttgcttg cagtgaaagt
     3001 caatgccgag tcagaaagaa aaggcttgaa cctaagcaac ccccaatcat ggaaccgaac
     3061 gcatgctaag ataacggcca tgcaacctct cgacgaggct gtcacgaccg cacggcttct
     3121 ttctgttgcc gtagacgcgt gcaagtcggc gggtggcctg ctgctggact atgcccggtc
     3181 aggatttcag atcgaacaca aaaacgcgat tgacctagtg accgatgccg accacgcggc
     3241 cgaacagtgc atcgtggatc atattcgcgc gcatttcccc actcacagca cgctcgccga
     3301 agagcgaggg catgacgcac ctcccacatc gcgctatcag tggattatcg atccgttgga
     3361 cggtacgacc aacttcgccc atcgattccc ggtctatgcg gtctcaattg gggtggaatg
     3421 gaacggtcaa ggggtcgtag gtgtggtgta cgatcccact cgcgacgaac tattcaccgc
     3481 ggaagccgga cacggcgcgc acctcaacgg tcaaccgatc aaggtttcga gcacaaccca
     3541 actggacggt gcccttatgg tcactggatt tgcgtatgac attcgggaca cagccaacaa
     3601 taacctgaac cactttgcac ggtttgcctt gaagtcacag ggtgtgcggc gaacagggag
     3661 cgcagcgctg gatctgtgtt atgtagccgc cggacgattc gatggttttt gggaggtgaa
     3721 gttgaacccg tgggacatgg cggcgggcgt cgtcatcctt cgagcagccg gcgggcgcgt
     3781 gaccgacctt cgaggtcacg agcactcgac cttccagccc gagctcgtcg caagtaacgg
     3841 ccggattcac gatgaaatgc tatccgtgat cagcgaaagc ctttcttcct aaccgctttc
     3901 accgcctctg gtcgcagagt tttggcatgt acagcgcaat ttcccctgcg ttatgatggg
     3961 cccataaccg gaaggagtgg tcgatggcca gcactatccc tccatcagga tcctcaaagg
     4021 gcaatccgca atcggcaggc gcggagtcga cctcgtggga cgtcgagcta ttgcgcgtct
     4081 tagtcagccg gaacggctcg caagtcgaag ctcaatggtc gatccatcct caactgaagc
     4141 aggatctctt gccgcatgag tggcaggaaa tcaccgatct gatgggaaag gtgtccggga
     4201 tcgtcggaac ccgcttctca caggtgctgg ctcaggccga tcctgaaccg ccgaccaacg
     4261 cctgacgcgc aatcaatccc agtcaatcac ctctatcatt ggagcgccgc atggacacct
     4321 attaccaccc gcacgatctc ggcaagttta gcgacatgga aaagggcaac aaggcactgt
     4381 gggacaagtt catgagctac tacagcgcgg tgtttgcgga aggcgccctg accgagcgcg
     4441 aaaaggcgct gatcgcactg gccgtcgctc acaccgtaca atgcccttat tgcatagatg
     4501 catacacgca gtcgtcactg gagaaggggt caaacgtaga agaaatgact gaagcgatcc
     4561 acgtagcctg cgccatacga ggtggagcat cgctcgttca cggcgtacaa atgcgcaacg
     4621 tgaccgagaa gctgtcgatg tgagaaggat tcggaatgtc tgccctatct ctagagatag
     4681 ggatgttccg gaatggccaa ggtgaagtat ctcccccgtt cttgatacaa gatccgtctc
     4741 gaggcgagat cagtcaaaat cggttctaac tgagcttggc ttgtgtccag gtaggcaagc
     4801 gccgacagca gttgctccgc ggtcttcggt gcctcattgc acatctcaat gaccagtgcg
     4861 gccacgccat cgtagcgctg ccgcgtgggc gcttttggat cgcgcccatc gtagaccgtc
     4921 acatacccca gcgccttcga ataatacagg aacggccgat cgctggtggc atgcagccgc
     4981 tgccagcgtt cgatggtgga aaccagctcc tgatacacat gggggtcaac gcgccaattg
     5041 tcgagctcgt attcaaagtc ataggcaatt ttcttcaggt cgacttgccg cgcatcgtac
     5101 acatactcat aggcagagcc gggaccggtc atacgcacgc cgtactcgga gggtcgtgca
     5161 tagtaggggc tgaaccgctc caaccagaat ttgccggtgg cttcaggcgg ctgcaggtgg
     5221 agcaaggacg ggatcatgtc aatctgtcgc aggtagtctt cattggtttc gcctgggaag
     5281 cccaacagaa tattccagga gactgtgaca cgatagtaaa aactccactt caggcacacg
     5341 atgttctgca tgggggtgac gcccttgtcc atcgccttca actggttcgc actgagactt
     5401 tccagtccgg gctgcataca cttcaccccg ccagcggcca acgttcgaat ctgctgtttg
     5461 tgaaggttgc tcttggtctc gatgaagacg tcaagatcgc aatggtcggc ggcaaacttt
     5521 ccaaaaagtt gatcgatata cttcatatcg ataatgttat cgaccagcct gaaccgcgct
     5581 gtgtcatacc ggtgagagag gtaggtcatt tcactcgcga cctgttccgg agatttcgca
     5641 cgaaacttca tgctttgagc attgagtccg caaaaggtgc aatgatgttt ctctccccac
     5701 caacagccgc gcgacccctc gtacaagaga atgcgatcca ggccttgcgc cgtagccccg
     5761 agttcagcta acaaacgata gtaatcgtca taatccggcg ggcccgtccg tgcgaagtcc
     5821 gtaaagagag cggtattggg cgctaaccga atctctccat cctgccgaaa ggcgactgcc
     5881 ggcggaacac cgtctgaacg cgttgacaac acctgtcgaa ccagagcggg aaaggctact
     5941 tctccttcgc cgacgaccac atagtcgata aacgggaacg cccgaaaata ttccagaccc
     6001 atttctccat cgtagttcgc tccgccaaac acgatggtga cgtcagggta gaggtccttg
     6061 atcaacttgg ccatcgtcag acttgccacg ttctgatcaa acgtggaggt gaagccgatc
     6121 agcttgtatt ggctccaatc aaacgccgtc aaagcccacg tcagaaactg cggggcgatc
     6181 ctcgtggcca tctcctcaaa atagccgatc ggtcggccac tctgctgcgc catctgttca
     6241 aagacaggct tgaagacacg cgggtattcg gcacgcttgg gattctcacg aaagaggaga
     6301 taggaaaaca gccactcacc gaagagagcg cgcttttcac aaatcaattc gtacagcggc
     6361 acgccgatct tgtgggcaaa atgaacgttc aaataatggc aatcgacggc aagcccttgt
     6421 gctttcagca atgaggagag cgtgccaagc tggatagagg gatacttcga gaagctgaac
     6481 ggcatgttga cgagcgcgac ttgcgccttg cccgtagaat ctactgtatg attcacgctg
     6541 tgatcacccg cctgccatgg ctcgaaacgg ttcaaattcc ctgtccgcct tcgccgccaa
     6601 gtaaaaatcg ctttcggtca atccattgat cttgtgtgtc caaatttcga ccttgcactt
     6661 accccatgac aaatacagat caggatggtg accttcggcc tcggccacgg caccgactct
     6721 gtttacaaaa gacagggcct gcgcaaagtc cttgaaggta tacaagcgct cgaggcggcc
     6781 ctgcgcgttc aaggtccagc ccctcccgag ttctttaagc aacgcttcta ttcggtcagc
     6841 gggaagcggc ggcacgccgc cgcggcaagg gatgcatttg ttgtcagcaa gacccatctt
     6901 ggcctccctg ttaaatggca cttattattg tacgaggggc ctttggatag aaacaaggcg
     6961 gggacagcta gtcgctctcg tccggcatcc cgcctgattt ggcaagatcg tccaccttca
     7021 ctttttccgg attgaatttg gcggccgcct ggatcatgcg ttccatggct tcgtgaaagg
     7081 agacaggggg agcatccttc ggccgaaagc ggagtggatt cacgcgggcg acaacaaagc
     7141 ttttcaagta gggactggtc agtcctttcg ccttcagcgc attgacctgt ctcacgatgg
     7201 catcgtcaag ttcgaggagc ttgcgcgcgc gttcctcccg cgactcgaat gccgttcgca
     7261 tcggcttttt aagaaactcg tccacacgtt tgaggacagg atggtaggcg cccccactga
     7321 atcgcggccg ttcttcataa cagagaccga gcgtaatgag cgccggttct tcgaattcca
     7381 acgcgtaagt ctcttcttcc gtttccggga gttgcatgag ttccttgtac atgcgaataa
     7441 cctcgagcgc cttttcccgg agattatggg ctttctcggt attgagagcg agaatgtggt
     7501 aggcggctgc ggcttcgggc acgacgatcg ccacgatgct cctcgcgccc agtgtccgca
     7561 tggctgagag ccgatgattg ccgttcggtg tccaatactt cgtcgtctga ttttccttgg
     7621 cgacgcggac gcaaatgatc ggatcgagaa accgggcgat cttgctgatc accgcttcga
     7681 gttttcttac atgggtatca gacaggttgc gctgatacgg agtcggttca accagctcaa
     7741 ttggcaacgc cgccaatacg acccagtgac ctccgtaagg ctcgcgatag accgaaagga
     7801 ccttgccgcc atcttgctcg atggctcgat gcaggtcggc cacctcggca tgtggggcag
     7861 cggcttgcag ttccgttgcc gtcaaggcgg tcgactgtcc tgccggcttt cgtctgcgcc
     7921 gagttccggc cacgcgtggt ctctttgttt gcttctcggc catgggcgga tatggtagga
     7981 gagtcaaccg acaaaagaaa ggcctcaact atgtcgcgcg gcggcatgcg tattgaaaca
     8041 atcggctggt tcaccgccag ttgtctgctc gtcagcaata tcatcggggg cggcgtcttc
     8101 acgaccacgg ggttgatggc acgagaactc gggaatccga tgttcatcct cttgctgtgg
     8161 gcagtcggcg gactattcgc tgtgggcggg gcgatggtgt atggggaact tggcgcgcag
     8221 ctcccccatg ccggaggcga ttacgtgtat cttcgtgagg cctatggacc attagtagct
     8281 tttctcagcg gctggacctc cttcacaatc ggcttcggag ccgccgtcgc cgcatccgcg
     8341 atcagctttt cagcctacgc gttcagaatc ttccctgtaa tggacgaaag cgggtggtgg
     8401 gccaaaggac tgtcgctcgc gctgttgtgg ctcgtgacgt tcgttcattg ccgcggattg
     8461 gcgggtggag ggcgattgca gcgcatcatc acgagcacga aaatcgtggc gattggcgcc
     8521 cttattgtgg gtggtttgtt cgcaagtgcc gctcaatggg actggctgtc ggtcgggcat
     8581 cctgcagcaa ttcccaaagc cggatccatc gccgtcgcgt tcgtcatcgt gatctattgt
     8641 tacctcggat ggaacgtcgc gggcttcatt gctcctgaaa ttaccgaccc acgacgcacg
     8701 ctgccgagag tcatgatcgg cggcaccgcc ttcgttgcga cgatctatgt gcttctcaac
     8761 tgtgtgtatc tgtccgccct atccgtcgac gggttgactc aggaacccat cgtgccggtc
     8821 gcggagaaat cggcgacggc attgtgggga ccaagcagcg gacagttggt cgccgcgctg
     8881 ctctgtcttt ccattgccgg agccgtgagc gccatgacgt gggccggtcc tcgcgtctat
     8941 tgggcgatgg cacaggatgg ggtgctcggt tcatggctcg cccagcgtca tccggaaacc
     9001 ggcgtgcctg cgcgtgcaat tatgcttcag acactctggg cttcagtcct tatcctcagc
     9061 ggtaccttcg aacaactcat cgtgtatagc ggcctggttc tggcattctt catgtgcctg
     9121 acgctctccg ccatcttccc cctccgatcg agacctgacc ggcctgccgg tgtctaccgc
     9181 atcccgggat atccgatcct gcctgcagta ctggtcatgt cctcgttact agtcgtgtgc
     9241 tcgggtctgg tgcagcgacc catggaggct atgtatggaa tggtaacggt gatggcgggc
     9301 gtcccttttt acgtattgcg gaagagaaaa agccaagacg acttgcgcga ggcggcagag
     9361 aacaaatgat ggggttatcg agaacggaga agaagcgcgc atgagtggag cgcacggatt
     9421 ttcatgacgg aatatggcac gggcgacctt gcaacggtgg gaggtcgccc gtggtttggc
     9481 gcttaggacg ccggactgaa attgggttgt tgcgagtcga aagacccgcg cgatgtcagg
     9541 ttatgcgagt gtctcgtcgt gggagacgtc gtcaatccca ggcgcttgtg ttcctcgcgc
     9601 cgcgacggat gctccgtaaa tcaggctcat atccggttgg tctatagtgc catcagggca
     9661 cagccaggag gatccggcta gcacgatcga cgagaaaccc gccctcgcat cggcgggcct
     9721 gacggaccag gtggagtcgc gtcgtccata tccaccctcc tctcttggca agtcaccgag
     9781 agacctggat gacagccgcg cggtctgccc gatctcccga atcttcggtg acacagttaa
     9841 gctgcagatc cgtcctacgc tcattagaca attgtgtcaa tgggtccaga cctgaccggt
     9901 gaccagcata acacgtgcca ccgtcgcaga gctgaaacag cacctcatag tttttctaaa
     9961 cgatcttgag cttggcaaaa tctacaccag aaggtggtgg tggggtcttg aggttcatgg
    10021 cttccaaggc ctgggcgatt cgatcggcca cgacaaggtt tcgataccat ttacgattgg
    10081 agggcacaat gtaccaaggc gcttcttcgg tgctcgttgc agacaagaca tcttcaaatg
    10141 cttgcatgta atcagcccag cgttcacgtt cctcgaggtc gccagagttc catttccatt
    10201 gcttttcggg gtcatggatt cgctcctcca accgtttctt ctgctcgtct ttcgaaatat
    10261 ggaggaaaaa cttcaggatc gcagtgccgt tctctgtcag catgtcctca aattctctga
    10321 tctgatcgta tcgacgcttc accaccgtct tggacaccca cccatggacc cgagtaatga
    10381 gcacatcttc gtaatgggac cggttgaaga tgccaatgaa cccccttgga ggcacctcgc
    10441 gatggacacg ccagaggaag tcccgggcca attcttccgt tgagggagtt ttaaaggcga
    10501 cgaccttaca accctgcgga ttgacgcccg acatcacctg cttgatggtg ctgtctttcc
    10561 cgcttgtgtc cataccctgg aggacgatca agagcgctcg actgttgttg ccatatagtc
    10621 gctcttggag tcgactcact cgagcgatat gtttctccgt ttcagcttta gcggccgctt
    10681 tgccttcgtc agttttctta tagtcgccgg tgtcatcggg gtcatggtct ttcaacgaca
    10741 atcggctggc cggcttcaca cgataccgtt tcatgatctg ccgtcaggaa gtggaatgcg
    10801 ccgcggggcc aatcgagcct cgcgttgtct agggctgctt tggcacatct tgtgcgcagc
    10861 gaaagccgac gtttgggtcg tgttcggtgg gatctccctt ggtacggaag gacgagcgca
    10921 ggcggtatgc gccgttaata tatgacccac cacgttgcac tttggctgat ccttctgcag
    10981 gtcctcgggg attgatggct ggattcttgc tgtaaaattg ctcgtcgtac caatcggtcg
    11041 tccattcata caggttgccg gccagatcgt gcacaccgaa agggctcttt cctttgtcgc
    11101 gattgccgat ggccgagacc gtatcggagc cttccttcaa accaaagacc gcatgggcga
    11161 gggtaggagg gtcatttccc cagggataga ctcgcgcgtc cgagccacga gccgctttct
    11221 cccattcggc ttcagtgggg aggcgcttgc ccgcccagag acaatacccc atagcttcca
    11281 tccaattcac ccagcggaca ggctgatcct gacgcagcac aggagtctcc ttgtcggcaa
    11341 agccccaggg aggctcactt tgtatggctt cgacaaactt ggcgaatcgc ccattggtca
    11401 cttcaaactt atccagataa aaactgtcga ggaacacttt atggaccggc tcctcatcgt
    11461 cgtccccttt gtcgcttccc atggtgaact caccgactgg aatgagcacc atgggcgccc
    11521 cgtcctttcc tacgatctcc gggagcggct gcatgatgac aggggctttc tggggagaga
    11581 ctccattgga cgcagaagca gatttggccg gcggcttcgc aggcat
//
LOCUS       DASSCQ010000042        30780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_288682, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000042 DASSCQ010000000
VERSION     DASSCQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 30780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30780
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288682"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1400..1493
                     /locus_tag="VFH05_02985"
     tRNA            1400..1493
                     /locus_tag="VFH05_02985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1434..1436,aa:Ser,seq:gct)
     gene            1665..1740
                     /locus_tag="VFH05_02990"
     tRNA            1665..1740
                     /locus_tag="VFH05_02990"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1698..1700,aa:Phe,seq:gaa)
     gene            complement(2029..3102)
                     /locus_tag="VFH05_02995"
     CDS             complement(2029..3102)
                     /locus_tag="VFH05_02995"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6677205.1"
                     /translation="MLQKLGFSHSIPGLAGVVLLVVIASCQSYNTPLVTETDGSYLRL
                     PDNSTKALVWGTDPDAVKSLKTWLLKQRIPLIDDVKLNQLASEMGLHQPVSNPDVLKL
                     SKTLGAKQVIFVDADVYKSQGDDIAVVFRQAPVYKVSLFIRALNAETGEIDWSGKAQS
                     IESFTNLSDGIHQLTCHALATAWGLREAGRAGAPSVCLPGQNVMVRAEPPTARYILTI
                     AKAFRTVYAKQIVAQAARSNVKPDEDWTKDDHAIMLPAQFLKAAGRELEDFELSLIGL
                     TPVYKENLPKTKAEEDALKRLMANPATGMVTFEDGNQFKGLAADLAIADSCVACHNTH
                     PTSPKRDFKKGDVMGAIVVRFNK"
     gene            complement(3190..3651)
                     /locus_tag="VFH05_03000"
     CDS             complement(3190..3651)
                     /locus_tag="VFH05_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677206.1"
                     /translation="MATRKVFAKKSGKIFPREMVRIRTSGNLNPTASRSGQTITTVPA
                     RKVLVIETISANIRIPKVTPTPEVARFVVVTGSAFPSGTGAPLLDIPTTRMATDPAGN
                     FVNYVALASIRAYATGGIVQYSIDLSIPSSGSFDVSLFGYLLPADSQNWAP"
     gene            complement(3944..4726)
                     /locus_tag="VFH05_03005"
     CDS             complement(3944..4726)
                     /locus_tag="VFH05_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6677207.1"
                     /translation="MTRAQAARIRELFRDKRVRKAEGAFIIEGAKPVHDVLINYPTFV
                     QTLVVTEEYLQREGHQERLVRESVNVPTFRCLNRAFSALSDLETPQGILAVLHKPNWD
                     EDHVFAQARLLGLYGEKLQDPANVGAIIRTAAAFDANALWLTPESADVYNPKVVRATA
                     GALLSLPVFISRDVTRFSQRGCAVFAAEARGAETVKMETIREVPMRLVIALGNESRGL
                     SAHTINEATRRFTIPLNPRIESLNVAATAAIALHFFGGLSRS"
     gene            complement(4774..5115)
                     /locus_tag="VFH05_03010"
     CDS             complement(4774..5115)
                     /locus_tag="VFH05_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677208.1"
                     /translation="MLGTDIRGIMAEEEEVLRRRQALKSLLSMRTKQLRESLEQRIKR
                     ANNGGDWMQLSREECATLHKQEKAHLKSQLEQLQHEDDRTRGKLTLLKRAKARAQRIR
                     AAEAASGRKRR"
     gene            5268..5726
                     /locus_tag="VFH05_03015"
     CDS             5268..5726
                     /locus_tag="VFH05_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6677209.1"
                     /translation="MAIGLQIQAWRLSRGFSREYLAAEAGISLQALDDIEAESFDPST
                     KTLEQLATAIKIPVAWLFAHPDAFQLLFRDQDEVEETPDGPDPITERILTGSRMDRSL
                     YVMLTALIQSGEPKLLRAAEMSLKSLVKQSKQSSVPWQNRPSGHFEPPSD"
     gene            complement(5880..7073)
                     /locus_tag="VFH05_03020"
     CDS             complement(5880..7073)
                     /locus_tag="VFH05_03020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET6677210.1"
                     /translation="MTGDHELSTGTVTLARAKEVVGSGHLWIYAGFVHAVGGQPVAGD
                     LVDVLMPNGRFYARGFYNPMSKIRIRILAFLDERIGPAFWHERIAQAVRLRKRIVSSA
                     TAYRVIYGEADRLPGLIVDRYGDVLVMQTLSSGMDRRKEMLADLLCKETGLSKVYLRN
                     DAKSRLLEGLRPVTGFLCGNGATTIEVQEGPARFLVDVAHGQKTGWYCDQRENRLAAA
                     RLASGQAILEVFCHTGAFGIQAALAGAGSVEGLDVSEEALVVARDHARLNDVQDRCSY
                     RSADAFDEMRRLVHARKQYDVVILDPPAFARSKQAVPRALAGYKDINLLGFRLTRPEG
                     IIVTSSCSHHVSEMDLWKVLTQAAQDANRPVRLLEQRGQASDHPVLLTMPETRYLKCF
                     ILQVL"
     gene            7211..8866
                     /locus_tag="VFH05_03025"
     CDS             7211..8866
                     /locus_tag="VFH05_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET6677211.1"
                     /translation="MGTEATIGSAVIDRLHQLGVRHIFGIPGDYVLSLYELIERSPIR
                     HIGTTREDCAGFAADAYARINGIGAACVTYCVGGLNCVNAIACAYAERSPVVLLTGSP
                     GLSERIRTPYMHHMVRDFSTQREVFERMTVAAISLDDPSTAEREMDRAFAALLRYRRP
                     IYIEIPRDLVHTPLLHPLRPLCLNEDPSDPAALEEAIGEVRAMLASAKRPAILAGAEI
                     GRFGLQDDLTRLVERLKIPIASTLLGKSVIREDHPLYVGVYGGLIARDEVQQFINDSD
                     CLLILGSILSDLEDLNADSPLRSEGRTIHATADHVSIRHHRYDTIRFQDFVQALGTHG
                     LSAQSSRPLPPPLSITGPPPAADSQVTLKGLFGHLDLKLDQTTLVIADVGESLFAAAD
                     LRVHRGFEFLSPAYYTSMGFAVPASIGASFADPTLRPIVLVGDGAFQMTGSELSTAVR
                     YRQAPIVIVLNNRGYSTEREILEGPFNDIHDWRYERICDLIGGGIGVVVRTQQEFEQT
                     LEKALADSGQLYVLNVLLDPGDRSPGMIRLAHRLAKRLSTDRP"
     gene            complement(8979..9185)
                     /locus_tag="VFH05_03030"
     CDS             complement(8979..9185)
                     /locus_tag="VFH05_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677212.1"
                     /translation="MQYWVKIVFVDNQELLVKDAVRHTISDDMEVLEVDSAKEVIIVP
                     MKQIKYLACDATVFASKGPPQPKG"
     gene            complement(9201..10055)
                     /gene="proC"
                     /locus_tag="VFH05_03035"
     CDS             complement(9201..10055)
                     /gene="proC"
                     /locus_tag="VFH05_03035"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET6677213.1"
                     /translation="MKRFKARQYNCAMLSGRTIGFIGAGNMAEALISGLLHGKRITPA
                     QLLASDINASKVARLRENYKIEAASTNRDVAKQADVLVLAVEPQVLDEALADLGVVIR
                     EETLLISVAAGYPIARIAHYVPGTTQIVRSMPNTPSLIREGVTAIAYDDRLSDAHASI
                     VRALFESIGQVVKVEEGALDAVTGLSGGGPAYVYVMIEALADGGVKAGLPRETAQLLA
                     AQTVSGAARMVLELREHPGMLKDRVASPGGTTISGLHELERGGLRATLISAVEAATRR
                     SQELGTTI"
     gene            10160..11233
                     /locus_tag="VFH05_03040"
     CDS             10160..11233
                     /locus_tag="VFH05_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6677214.1"
                     /translation="MRQLALSSLVLLVLLVSNSWARQESLTAEEKQKLEKIDRVLVEV
                     IAISDKGSTDAGPLTDVVVKRMKEFGYTVMTDPSQPHDVTLNVKCEQRKIWEGTTKMG
                     SDADLPDSPSRLWKGPACQLSYVLEGKKMPWRKEVRTDFTDAEQAAVVANAGDPSAFA
                     MNKLKERLEDYDFPALITAEWGQEERLFKLYDDPSTSSARKVRLIGLFGYLFETKAVP
                     RLLAGLKDKDIEIAKASALALGNIGQKDSVPALVQAMTSGPAELRPSAAKALGVLGAL
                     HGDFSIVEPLLETLKTDDVALKTEVAWALGKLPDRKAYEPLYALQKSLHQVRDHDADP
                     KVVKLKEAVNWSIKQIDTWEYLQ"
     gene            11276..11776
                     /locus_tag="VFH05_03045"
     CDS             11276..11776
                     /locus_tag="VFH05_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677215.1"
                     /translation="MKLSTVLIGIVSLACITESAWTQTMGDEAELERLRVKAEEAIAS
                     DDPEGAAMNMGRAALMAKRLGLLSRDNHASVRLYQGAESLFRSQEHGYRAMALFRRAG
                     GQLPASSGVCGSLALAQGSVQRALLDLSSESLPVSLVERATRWRETADDWVIVVAAMI
                     TDYQCP"
     gene            complement(11802..12245)
                     /locus_tag="VFH05_03050"
     CDS             complement(11802..12245)
                     /locus_tag="VFH05_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET6677216.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRIVNKLMVKRGKVAILPTWTVTE
                     LYSAFSNRAQQGELTRDDCYSVIHKFERESAQGLFQFIVPTMHTYLSTKELMMEYPGL
                     RTQQVLHLALALELKPLRLTVVSADTQLLSASKTAGLHIINPEED"
     gene            complement(12249..12512)
                     /locus_tag="VFH05_03055"
     CDS             complement(12249..12512)
                     /locus_tag="VFH05_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677217.1"
                     /translation="MSRSKPGGRAPRSRGRGLTATQQRVPSRLAKRRLNERLKSDTAA
                     FNEGNLADKLRREGYEEVPLEELQDRLSKMPGPLAAVILKGRV"
     gene            complement(12538..13653)
                     /locus_tag="VFH05_03060"
     CDS             complement(12538..13653)
                     /locus_tag="VFH05_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HET6677218.1"
                     /translation="MFTALLVGCSGWGGPSKPSWMDGANSEYPSAQYLTGVGQGDNRT
                     VADDQAYAALARIFKAEISAQSQDWESYLVVEQHGTSHDERRLTLDHLTRVSTDKVLE
                     NAKILDRWYDAKKGLHYALGGMHRAQAETALIERMTELDRAVLSDVDEAGRTSDRLAK
                     VRALRRATRNVALRETYNADLRVVRTSGQGMPPRVRLQELSRELEEYLAAHLVLTVAI
                     TGDHVEPVQRALTEGLIKEGLRVAAVPSGTETASGAAPPLTPELLIRGMVRVWPVDIR
                     DPQFTYVRWCSDFEIVDLAGQQIIGAISKGGKEGHLSEREATAKVIRVMQQDLSADVA
                     RAIAAHVYGESELPGAANRPAGCPREALRARPANATH"
     gene            complement(13692..15128)
                     /locus_tag="VFH05_03065"
     CDS             complement(13692..15128)
                     /locus_tag="VFH05_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677219.1"
                     /translation="MSAHVLTFLCTAALGGCTFSQSHYLLVDKRLLTSDYQEADAIIR
                     QAEKDYGAKSRVLYGMDRGMTLQLAGEYEQSNRVLEEAEEEVDRLYTRTVRTETAAFM
                     TNDNTLPYEGEPYEQVMINVLKALNYASLNRWQDALVEARRMDHRLNVLADRTKDKAA
                     YHDDGFARYLSGILYESTGDVNNAFIAYRKAYEMFDSSRGWSRTGVPAQLQADLLRTA
                     DALHLSQEFSDYQRRFPDTAWTSLETQQQLAQVIVISYNGRAPRKEDQFLDVPISLDA
                     LQLVLLNRGLFQGNRQQHRAADTALYGLNGRVVRVALPMMVPQKTQVRTDTIRLLPAD
                     GPPLMVRTELVQNVTALASKALAERLPGITVKAVARAALKFSMAEAATRGAQQAAGRD
                     AAPWVGLAVGLLTKGFAVASEESDKRSWRTLPDEIHLARAWVSPGEYRVQGAVADVGF
                     RLMTPASSEKTLSLQAGETVILIQRVMH"
     gene            complement(15203..15826)
                     /locus_tag="VFH05_03070"
     CDS             complement(15203..15826)
                     /locus_tag="VFH05_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HET6677220.1"
                     /translation="MSIMTQRIQWTKPAVLMCLLVGLSACGGETRVTRIDAGVVTDLS
                     GRWNDTDSRIVAESMVKEALEYPWLNTFSQSKRRQPVVVVGTVLNSSHEHINVHTFVT
                     DLERELTNSQKVTFVAGKNERDEVRSERKEQAMYAREDTQKAPGREIGADYIMKGTIA
                     TILDEADGTKAMFYQVDLQMVDLESNAKVWFGQKKIKKVIEKKRTIF"
     gene            complement(15851..16414)
                     /locus_tag="VFH05_03075"
     CDS             complement(15851..16414)
                     /locus_tag="VFH05_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677221.1"
                     /translation="MKPRGCFSNVTCGGMALILLLALTACGGPPKWVKQGSGAFNEKD
                     TKAFYGVGSVTGVKNEPLAWDTAENRARAEIAKTFETYTGYLMRDYAASTTAGDFTRN
                     TEEQNVERAIKTVTTTTLSGVRPIERYKDEKTSTYYVLTKLNLEEMKNNLEQAKELNA
                     QVRDFVRKNADKLFERLEKEEDKRANH"
     gene            16579..16749
                     /locus_tag="VFH05_03080"
     CDS             16579..16749
                     /locus_tag="VFH05_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677222.1"
                     /translation="MADVQIDDGIIKILNLDVQDPKAAAVLAEYPQVRWAEITRRALK
                     IGLGYLKGGARD"
     gene            complement(16746..18140)
                     /locus_tag="VFH05_03085"
     CDS             complement(16746..18140)
                     /locus_tag="VFH05_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET6677223.1"
                     /translation="MMLPTSPPSLQSRFLAILVVLAMTGLSPVAALAQMGKPEGLYYK
                     SWAVVIGIENYLLAPKIPGVIEDAKAVAEAFRKLGFEEVVELYDKDASSRRLQQTLVD
                     FLPRKVGRQDRLVIYFSGHAGITQSGTGKDLGYLVPWDAQPGNVSKAVTFEQLKEFSH
                     RSASKHTLFVFDTAVRGWEASAAQALSLEGRSAPEDDTEKRAVQVLTAADKGEAAVRT
                     QDRSVFVLALIKALTGEGDIDKNGWLMASEVGRFISQQVTDDTKGAQHPTFAQLEGDG
                     DTIIIEGRKAAFRLGGEPQSPAERQQAARMQYEQAFALLQNGKAQDEALERLNKSLEY
                     DPTFGDAYVLKSYVRLEVIPDLEEALKAGKLAVQYAPNNPDSHYTLGLIYEKRGQYAE
                     AEEAMRRALAANPSYVDVYFTLGSLYADHLKDQKKAVEAFERYLELGGTHNRAREVVA
                     QSRATDTQPSSKTP"
     gene            18317..20206
                     /gene="acs"
                     /locus_tag="VFH05_03090"
     CDS             18317..20206
                     /gene="acs"
                     /locus_tag="VFH05_03090"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET6677224.1"
                     /translation="MTENIETLLKESRVYRPTAKTIAAAHIKDYEKEYQKSIADPEAF
                     WSGMAKELDWFSPWSTVLEWNYPWAKWFVGATCNITYNCLDRHVKTWRKNKVALIWVG
                     EDDRERIFTYGELYRQVNRCANALKKLGLNTGDRVTIYLPKIPEQLIAMLACARIGVI
                     HSVVYSGFSAPALASRINDAESKLVITADVGFDRTKVIPLKPIVDDALKQCSSVERVV
                     VVRRQPSSPLPTAPKELDWTDWVKGEPAVCEAKPLDSETPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYTTTKYVFDMKDDDVYFCVADPGWVTGHSYIVYGPLLNGATVLTAEGKP
                     DFPNPGRWWDLIERYGVSIFYTTPTAIRLLMRHGEDWPKKYDLSSLRILGSVGEPINP
                     EAWEWFHRAAGGDKPIMDTWWQTETGSILITPLPTVALKPGSATRPFLGIEADVVDRQ
                     GNPLPANQGGFAVIKKPWPSMMRTIYKDPERYKTYWNIIPNCYTAGDVCHKDADGYMW
                     FMGRADDVIKVAGNRLGTAEVESALVSHHAVAEAAVIGIPHKTVGETIKAFIILKQGE
                     TANPALIESIKEQVRHELGKIAVPSEVDIVTSLPKTRSGKIMRRVLKAKELGQDPGDI
                     STIEE"
     gene            20258..20734
                     /locus_tag="VFH05_03095"
     CDS             20258..20734
                     /locus_tag="VFH05_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET6677225.1"
                     /translation="MVKRMGRTMMLIVAVGFLLVTGDPGLAPAADKEKPATKPKAAPE
                     AKAPKPKAPKTDKPKFQTAEKAGAAKACFGEAPRITKLTPDEGKAGDKVTITGTNFGS
                     ADCLRGVSFGPGHAATFKVKDETTITATVPTARRKGLAIVTVTTASGEDSKAFLMK"
     gene            20995..21219
                     /locus_tag="VFH05_03100"
     CDS             20995..21219
                     /locus_tag="VFH05_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677226.1"
                     /translation="MQGGRRKPGEPIYLRRHVIALALAVGLPIIALQIYKIYVGPLHF
                     IVQIGLAAFASVVAGLWLYVSYRSSAQNEP"
     gene            complement(21271..21834)
                     /locus_tag="VFH05_03105"
     CDS             complement(21271..21834)
                     /locus_tag="VFH05_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET6677227.1"
                     /translation="MRCSLFSSTPPPGFHYLPDWITVEEENALLSQIERLSFSEVRMH
                     GVTAKRRVVHFGWDYGYDSWTIEPTEPIPDWLMPLRLRAGALMGVPAEAVEEVLVTRY
                     EPGAGIGWHRDAPMFGPMVVGVSLAGTCRMRFQRKVAGERETAEAILAPRSAYLLAEE
                     ARFLWQHSIPPAKELRYSITFRTVKAR"
     gene            complement(21900..22553)
                     /locus_tag="VFH05_03110"
     CDS             complement(21900..22553)
                     /locus_tag="VFH05_03110"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET6677228.1"
                     /translation="MFTGIVEEMGAVTAIEKTLAGTRLTILASAVLGDLKVGDSVSIN
                     GTCLTVVAKGETTFVVDVSPETLSVTTLGELVGGTPVNLERAMKLNERIGGHLVAGHV
                     DAVGTIRSRHQEGNAIVLSIEAPPDVLRYCVSKGSVTVDGISLTINHVTDSSFSVAVI
                     PHTATATTLGLKQVNDTVNLESDLIGKYVERLLQQRSQLPKMTPAIDKDYLKKRGLL"
     gene            complement(22756..23922)
                     /locus_tag="VFH05_03115"
     CDS             complement(22756..23922)
                     /locus_tag="VFH05_03115"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6677229.1"
                     /translation="MTTARSFTLLCTIGVFCFISYNMVRMPVLALFAESLGASPERIG
                     VIVSVSTLTGVLLKLPSGALSDIYGRRILLRVGVIAFGLPPFLYPFISDLNLLTLLRL
                     FHGLATAIFAPSALATVAELYRERRGAALGTYTACTQSGALLGPFIGGYLAFASGFSS
                     AFLTAGFFGCIAIVLFYSLRLTPPPLRNQEKGLAPVVTEMWKGFSIVARNRKVLITSS
                     TDAAKMIANGALMAFLPLYGVGVGLNAGEVGLLFTVQSLTSFISKPIMGRVSDRVGRQ
                     PLIMLGLVICAVTFVCIPHVASFAVLVLLTAGFGFGEAVVSSSSSAFVADSSEFKTLG
                     AGMGMQGTIGDIGHASGPLLAGVLIAHTSYASAFTTIAGLQVAAAGFFWLTMRK"
     gene            complement(23919..24734)
                     /locus_tag="VFH05_03120"
     CDS             complement(23919..24734)
                     /locus_tag="VFH05_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677230.1"
                     /translation="MNIGDRLLHGNRSRFFWLILALLGSISLGRARAESCFVASEQSG
                     IAFPVGKIAGDWTCRLQFVIDHHLSANTVGPVKTVMAEPLYLYLLDRPPVAAALINRL
                     DLADYKSEERGPGRWWGSDGEGTEGIVQLVYQDRATRVYYLEGTHHSRLLPNVSGKAV
                     IFLRMRPVKDERGSDAMESTMVAYTMLDNRVLSGFVSLLRPLVDATVMRKLSKGVEVV
                     NRLGLEMGQRPDRVLFEATDPPPLPKQDVAFLTQALSRRPTSSATPNSSRPAP"
     gene            complement(24731..26017)
                     /locus_tag="VFH05_03125"
     CDS             complement(24731..26017)
                     /locus_tag="VFH05_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6677231.1"
                     /translation="MRPWLAAVGVLALAVGLETSLTMARVDPPQSLSTLVFHYLDSAD
                     SEEAERFLKVLLNHPDISVERVTRIIETERTYDAQPIGTVANEGVRVEGQTYAVALSI
                     PLTYRSANGYALVVCLHGAGFTGDVYLERWQARLGEEYLLACPTYPSGAWFTRRAEEV
                     VLATIEHVRRRYHVDPDRIFLTGMSNGGIGAWLIGMHHAPLFAGVAPMAGGLDDVLMP
                     FLANLRNTPVYMIHGEKDQVMPVSLSRSISRELTALGYSHVYREHQGEHPVAGGHYFP
                     REELPALRTWLDRQRRDPAPTKLALVREASRFQPFSWVRVDATDVIAAFSDDLIDKRD
                     QRIKQREYAKLDAAIVGPNRIEVSTKHIQRYSLFLNDRLIDTAKPVSIWTNGQLSYDG
                     PVTVSIDILLRQARLRHDPRQLFPIHLSVLVPNQAS"
     gene            complement(26014..27135)
                     /gene="ribD"
                     /locus_tag="VFH05_03130"
     CDS             complement(26014..27135)
                     /gene="ribD"
                     /locus_tag="VFH05_03130"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET6677232.1"
                     /translation="MRAGHAQDLHYMRLALALAANGRGTASPNPMVGAVVVSHGRIVG
                     KGYHLRPGLPHAEILALKAAGPQARGGTLFVTLEPCCHLNKRTPPCVPAVIWAGVTRV
                     VIAQRDPNPLVNGKALQALRRAGISVTVGVGKSEAIELNRAYNHYMRTRRPYVLLKAG
                     MTLDGQIATASGEAKWITSVPSRSDVHRLRSEVDAVLVGIGTVVNDDPSLTARVGPRF
                     KKLTSEQPLRVVADSRLRIPFKAHILKQQHVAKTLIATTRSAPAARRHALTKLGVEVV
                     VLPSLRGRVSLPALMRELGRRGVTSLLVEGGSEINAAMLRAKLVHHLRLYMAPALLGG
                     SNAKGVIGGESPATLAETLKLKHVRTRWLGGDIVVEGDL"
     gene            complement(27251..30028)
                     /gene="ppdK"
                     /locus_tag="VFH05_03135"
     CDS             complement(27251..30028)
                     /gene="ppdK"
                     /locus_tag="VFH05_03135"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET6677233.1"
                     /translation="MAKKYVYYFGDGKAEGTSNMKELLGGKGAGLAEMTNLGISVPPG
                     FTISTEACVEYYKHGKKYPPGMWEASLQALKRVERSMGMGFGDPERPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVEGLAAKTRNDRFAQDSYRRFVSMFGSIVMGVPREHFEAI
                     LKHKKAEVGVTHETHLDARHLRELVASFKALVKEETKKDFPDEPLEQLRMAINAVFSS
                     WFGARAITYRRLYGIPDSWGTAVNVVAMVFGNMGETSGTGVAFTRSPSSGDKAFFGEC
                     LMNAQGEDVVAGIRTPLPVNALAKNVPEAYRELEQTYKKLEKHYRDMLDLEFTIQEGK
                     LYMLQTRVGKRTGIAAVKIAVDMVKEGAISREEAVQRVGPDQLAQYMYPIFDTKEESK
                     ANPLGKGLPAGPGAAAGKIALTPDRAVDMKAAGSRVVLVRQETSPDDIHGMNAAGGFL
                     TARGGMTSHAAVVARQMGKVCVAGCDSVEVLDSQTVKIGAKTFREGDYLSINGSTGNV
                     YEGDIPVVDSEIIQVIQGKLDPKKSEKYQRFSTILSWADSLRRLRVRANADVPEQAKI
                     ARGFGAEGIGLCRTEHMFFAEDRIPLMQKMILARTTEEREKYLDQLLPLQKQDFIGLY
                     REMEGFPVTIRLLDPPLHEFLPKREDLMVEIAQLELTGRDAAKLEENRRLLARVEELH
                     EFNPMLGLRGCRLGITMPEITRMQARAVIEAACELAKEGKKIVPEIMIPLVGMVSEMK
                     SQKDLVREVAQETMKRYNVKLSYLVGTMIELPRAAVTADRIAEEAEFFSFGTNDLTQT
                     TFGFSRDDAAKFIDHYKSVNLMESDPFAVLDREGVGCLMSQAIAGGRKTRAGIKLGIC
                     GEHGGDPSSVEFCHQLGLDYVSCSPYRVAIARLAAAHAALAEADARKAAKATLRKATG
                     RKQSTARRKKR"
     gene            complement(30053..30550)
                     /locus_tag="VFH05_03140"
     CDS             complement(30053..30550)
                     /locus_tag="VFH05_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677234.1"
                     /translation="MIASSESDLEEEREAKIRRFASVVTAVLIVFCNVLLLFGVWVSG
                     VNLDELVKTPDLFDAKEDVCLRLSWERVKGAADPVRLCSEWIKLADPSGKPHQLTKDI
                     AVKRGPDGQYYVDRGVRPDFRLLGLALFVGVVLLFGMWARRYLVSRYRLQIGPADSRS
                     SSGLH"
BASE COUNT         6661 a         9044 c         8305 g         6770 t
ORIGIN      
        1 ggtcatgaat cgtattgctg cagcctaacg atcccatttc ctcgcagtga tttcaacgct
       61 atggctggct gctctaaagt tccaattatt ttggacgctt tttattagcc agagcggctt
      121 cagtctcgcc tctgacaaag caaacttagg acattaccca gggaggccat cgggtaaaca
      181 atggtcctct gaagaaggtg atccagtcgg acatgtaact agtcgtagca taagaaagta
      241 atcaaaaact cctctttcct catggccggt tttcccagcc tttgcgagct ggctctaggg
      301 acaaccttta ataacttgaa aaatgagtgt ttgtgactag agctttaccg ccttgaatcg
      361 cagcacttta tctgatttag tgatcattgg gcggaggtgt cgtttccggc atgcccaccc
      421 tggagcgaca agaggtctct gctccctcct gggagacctg cctccgcctc cctttgagcg
      481 caagactgcc tttttcccag attcaacact ccccctttta gtacaccccc tcatacctct
      541 taggtatttc caaatgtctc attcctctct caagatagag aacacatgaa gaatcatcga
      601 ttaactcggg agaaagtgat gttcaagcaa caaggaaaag tgggagccat tcgtagaaac
      661 gtacgcggca gagcttgcgt ctcgtgcgga agccggaatt atcagcttgt actccgcagt
      721 ctgaagcagc cgcaactggg aaaactgttt gctcgctgta gtcagtgcca ccagtcccgc
      781 gaaatcgatg aagaccttgg ccgcattttg tggatgtagg aatgtaatga gaattttgac
      841 atcaccgctt cattacgttc agagcaaacg ggcccgcaga aggcaaatac agaccgtgct
      901 gcacatgcta tccgtaagcg gagtcattag accgggacat gtcgagcccc gctattttga
      961 accttcgatt cacatcgata ctcttcatcg ccaagtctct ggacagaacg gcattctcaa
     1021 ggcgccaaca gccattaccg cctccagtgt atccgcaaat atcagcttca actttttcaa
     1081 gctacgccaa tttgttagag ccatcaaacc ttctagtctt tcattagagc aatagctttg
     1141 agctcttgag tgccggaagt ctggtgcttt ccaaagcttc ccatgaaacc cttgtacctc
     1201 ccacaaccgg tcaggtatga tcacgcctca ccgcgacaag gcaagggccg ctttgccatc
     1261 agaaggacat gttctctagc ctttggtgtt acgaccgaca gaggaggaga tttgtattag
     1321 cctgctagcc ggttcttgat cgttaatcgt caagttgtag agacaaggaa gctcacatac
     1381 acagcagtta ccatcttgtg gagaggtggc cgagtggccg aaggcggcgg tttgctaaac
     1441 cgttgtaggg gctaaaacct ctaccgaggg ttcaaatccc tccctctccg ccacaagagt
     1501 ggactactaa taaaattggc aaattgcctg attcctaccc aacccagaga aagcggcctt
     1561 tgcgccaagt acctattggg atgatgggag tgactgctcc gcgaacacgc ctaacttgat
     1621 tttcgacgat taatcctgta tcagtgccac tctttccact ccgtgccgag gtagctcagt
     1681 tggcagagca cagccctgaa aaggctggtg tcgacagttc gattctgtcc ctcggcacca
     1741 ccaagtcccc tattagccta tctatgacgg ccagtttgaa tttgtttttt tggtccatct
     1801 cctaatttga atgggccatc ccggtccgtg atgaagtgaa cgtgtcacca taccaccgtc
     1861 cagacaaggc gatcgttaac ttccatctct tggcctcgtg agtgcgccca ccgtctttta
     1921 cggtagcttt gcggccttgt tgtcactttc ctggattgtg cccaagccat tttaccggtt
     1981 cgtacactcg cgacaaggtc agatgagatg atgtgatgta ttgggaactt atttgttgaa
     2041 acgcacaaca atggctccca tgacatcacc cttcttgaag tccctctttg gactggtagg
     2101 atgagtatta tggcaggcca cgcaggaatc cgcgatggcg aggtccgccg caagtccttt
     2161 aaactggttc ccatcctcaa acgtcaccat gccggtggct ggattggcca tgagtctttt
     2221 cagagcgtct tcctctgctt tcgtctttgg aaggttctct ttgtagaccg gtgttagccc
     2281 gatcaggctg agttcaaaat cttcgagttc ccttccggcc gcttttagaa attgggccgg
     2341 aagcatgatg gcatgatcat ccttcgtcca gtcctcgtct ggtttaacat tggatctagc
     2401 tgcctgcgcc acaatttgtt tggcataaac cgtccggaac gctttagcga tggtgaggat
     2461 atatcgtgca gtaggcggtt ccgccctcac cataacattc tgtccgggca gacagacgga
     2521 tggcgctccc gcccttccag cttctcgtaa accccacgca gtcgcaagag catggcaggt
     2581 taattggtgt atgccatccg ataagttcgt gaagctttca atcgactgtg ccttcccgct
     2641 ccaatcaatt tcaccagtct ctgcatttaa tgcgcggatg aatagcgaca ctttgtagac
     2701 gggcgcctgc ctaaacacaa ctgcaatatc atctccctgc gatttgtata catcggcatc
     2761 aacaaaaata acttgcttgg ctccgagcgt ttttgagagc ttaaggacat ccgggtttga
     2821 aacgggttga tgcaagccca tttcacttgc aagttggttc aacttcacat cgtcgatcaa
     2881 agggatacgt tgctttagca gccatgtttt aaggctttta accgcgtcgg gatcagtccc
     2941 ccacacgagt gctttggtcg agttgtctgg gagtcgaagg taggagccgt cagtttctgt
     3001 aaccaatggg gtgttgtagc tttggcagct ggctatgact acaagcaaca cgacaccagc
     3061 gagcccaggg atggaatgac tgaatcccaa tttctgcaac aaataacgcc tccggtcgga
     3121 gcttggcagt taaaagaaag gtcttcacct ggggtggtcc ctagcaaact gggcgtctga
     3181 cgatttcact caaggagccc aattctgact atccgcgggt aacaggtagc caaagagact
     3241 cacatcaaag gatccactgc tgggaatgct taaatcgatt gagtactgta ctattccgcc
     3301 tgttgcatag gctctgatgc ttgccagggc gacataattg acaaaattcc ctgcaggatc
     3361 cgtcgccatc cgggtcgtgg gaatatctag tagcggggcg ccagttccag aaggaaatgc
     3421 agaaccagtg actaccacaa acctcgcgac ttcgggcgtt ggtgtcacct tcggtatacg
     3481 aatattggcg ctgatagttt caatgacaag tactttgcgc gctggcaccg tcgtaatggt
     3541 ctgaccggac ctcgatgctg tcggatttaa attgccgctc gttctaatcc tcaccatttc
     3601 tcttggaaat attttgccgc tttttttcgc aaataccttt ctcgttgcca taattctttt
     3661 ccttattggt gaaagatctc gtgcaagtgc attgggggcg aggcacaaaa tcgataagca
     3721 ggcatggcgc atgtcaccgt accgtttgct aatagtatcg acactaacaa ggccgagtcc
     3781 ccatccctta gactgactga ttgattaagc gtcctgtttt tggctcccaa tcaccaatca
     3841 cttgcgctgc cgctcgcaga ggaacgaaac tttccaaccg ttttttctga tggtcaggct
     3901 gcagaggttt cgacatcttg gagcctccct gcgattcccg gctttaggac cttgataatc
     3961 cgccgaagaa gtgaagagca atcgctgcgg tggccgcgac gttcagagat tcaatgcggg
     4021 gattcagtgg aattgtgaag cgccgggtgg cctcgttgat ggtgtgagcc gatagaccac
     4081 gactctcgtt tccaagagcg ataacaagcc gcatgggaac ttcgcgaatc gtttccatct
     4141 tgacagtttc tgccccccga gcttctgcgg cgaagacggc gcaacccctc tgactaaacc
     4201 tggtcacatc gcgggagatg aagaccggaa gggaaagcag cgcccctgcc gttgcacgca
     4261 caactttcgg attgtacaca tccgctgatt ccggcgtgag ccacagtgca ttggcatcaa
     4321 atgccgccgc ggtacggatg atggcaccga cgtttgctgg atcctgcaac ttctctccgt
     4381 acagtcccaa aagccttgcc tgcgcaaaca catgatcttc gtcccaattc ggtttgtgta
     4441 ggacggctag aatgccttgc ggagtttcga ggtcagacaa ggccgagaaa gcccgattga
     4501 ggcagcgaaa ggtgggcacg tttaccgatt cccggaccag gcgctcctga tgtccttctc
     4561 gctggaggta ctcctcggtg acgaccagtg tctgaacgaa ggtagggtag tttatgagca
     4621 catcatggac gggcttggcg ccttcgatga tgaaggcgcc ttcagccttt cgaacccgct
     4681 tgtcacggaa gagttcgcga atgcgcgcgg cttgcgcgcg cgtcaaaggg ctagcaaggg
     4741 aggacacgcg aggcgtggca cagtcctata catttaccga cgtttccgcc cagacgctgc
     4801 ttcggcagct cgaatccttt gtgctcgtgc cttggcgcgt ttgagaagcg tcagcttgcc
     4861 ccgcgtacgg tcgtcctcat gctgcaactg ctcgagttga gacttgaggt gggccttctc
     4921 ctgcttgtgc aaggttgcac attcttcacg tgagagctgc atccagtcgc cgccgttatt
     4981 ggcccgcttg atccgctgct ccagggattc acgcaactgt ttggtccgca tggaaaggag
     5041 cgattttaac gcctgtcgtc ggcgcaggac ctcttcctct tccgccatga tcccgcgaat
     5101 atctgtaccc aacatgatcc gcctccttga gaatgaattg tccgtattgt acccgatttc
     5161 attgctcatc ttccactgag acaatttcct cataagagat tgacaattca gcgaaaacat
     5221 aagttgtcca cagttgccaa tactttgggc ttcagatatc atcgcgcatg gcgatcggct
     5281 tacagatcca ggcctggcgc ctgtcacgag ggttttcgag ggagtatctc gctgccgagg
     5341 cagggatatc gcttcaagcc cttgacgata ttgaagctga atcgtttgac ccgtcaacga
     5401 agaccttgga acagcttgcc acagcaataa aaattcctgt ggcatggctc ttcgcacatc
     5461 ctgacgcctt tcaattgctc tttcgcgacc aggacgaagt ggaggaaacg cctgatggtc
     5521 cggaccccat caccgaacga atcctgactg gttcgagaat ggatcgatcg ctctatgtca
     5581 tgctgacggc actgattcag agcggggaac ccaaactact ccgagctgcg gaaatgagtt
     5641 tgaaaagtct ggtgaaacag tcaaaacaat cctccgtccc gtggcaaaat cgtccatcag
     5701 gccacttcga gccaccgagt gattagtcgg ctacataatg ccgcctgatc cttggcttct
     5761 tttgctcaaa aagctcacgg ctccgcatct catgcgccct ttacccatcc cttggctgtc
     5821 gcttgttcaa ctaagatcgc cacgatttgt aaacaccgca gcgaaacagc gtggcgatct
     5881 taaaggactt ggaggatgaa acacttgaga taacgggttt ctggcatggt caaaagcacg
     5941 ggatgatcgg aggcttgacc tcgttgctca agcagacgca ccggccgatt ggcgtcctgc
     6001 gccgcctgtg tgagtacctt ccacaaatcc atctcagaaa catggtggga gcaagaactc
     6061 gtgacgatga tgccttctgg tcttgtcagt cgaaatccca acagattgat atccttataa
     6121 cctgcgaggg cccgtggaac cgcctgtttg ctgcgggcaa atgcaggagg gtcgagaatc
     6181 accacatcat attgcttgcg agcgtgcacg aggcggcgca tctcgtcgaa ggcatcagcc
     6241 gatcggtagg agcagcggtc ctgcacgtcg ttcagtctgg catgatcccg tgccacgacg
     6301 agcgcttctt cactgacgtc caacccttcg actgagccgg ccccagcgag ggccgcctgt
     6361 atcccgaagg cgcccgtgtg acagaagact tcgaggatgg cctgtccgga tgccaatcgg
     6421 gcggccgcaa gccggttttc gcgttggtcg cagtaccagc cggttttctg cccatgcgca
     6481 acgtcgacaa gaaaacgcgc cggtccttcc tgaacttcaa tcgtcgtggc tccattcccg
     6541 cataaaaagc ccgtgacagg ccgtaagccc tctaacaggc ggctcttggc gtcattccga
     6601 agatatacct tgctcagacc ggtttccttg cacaacaagt ccgccagcat ctccttccgg
     6661 cgatccattc cactcgacag cgtttgcata accaacacat cgccgtaccg atcaacgatc
     6721 agaccgggca gccgatcagc ctcgccgtag atcacccgat aggccgtcgc actggaaacg
     6781 atccgcttgc gaagccgcac ggcttgggcg atacgttcgt gccagaatgc tggtccgatc
     6841 cgttcatcca ggaaggccaa gattctaata cggatttttg acatgggatt ataaaatccg
     6901 cgagcgtaga accggccgtt cggcatcagg acatcgacca ggtcgccagc caccggctgc
     6961 ccgccgaccg catgcacgaa cccggcatag atccagagat gcccagaccc gactacttcc
     7021 ttggcacggg ccaaggtgac tgttccggtc gacagctcat ggtcccctgt catgggacag
     7081 ttatcggatg gggcgagccc gacgtcaaga ccttggcctc cctcgtatct gcttgacggg
     7141 ttgcctcctc tatggtttgc tggaacgaga gcctcgtctc gcacaccata acgaaccgca
     7201 gacggaatca atggggacag aagcgacaat cggctcagcc gtcatcgatc gcctgcatca
     7261 gctcggtgtc cgccatatat tcgggatacc cggtgactac gtcctcagcc tgtatgaact
     7321 catcgagcgt tccccgatcc gccacatcgg cacaacccga gaagactgcg ccgggtttgc
     7381 tgcggacgct tacgcgcgca ttaacggcat cggtgcggcc tgcgtcacct attgcgtcgg
     7441 cggcctgaat tgcgtcaatg ccattgcctg cgcttatgct gaacgctctc ctgtcgtgct
     7501 gttgacggga tcacccggcc tgtcggaacg gattcgaacg ccctacatgc accatatggt
     7561 gcgcgatttt tcgacgcaac gagaagtctt tgaacgaatg acggttgccg ccatttcact
     7621 ggacgacccc tcgacggcag agcgtgaaat ggaccgcgct tttgcggctc ttctgcggta
     7681 ccgccgcccg atctacatcg aaattccacg cgatctcgtc cacactcctc tgctgcatcc
     7741 actgcggccg ctctgtttaa atgaggatcc aagcgatccg gccgcgctgg aagaagccat
     7801 aggagaggtc cgtgccatgc tggcatcggc caagcgtccg gccatacttg ccggcgcaga
     7861 aatcgggcga tttggccttc aagatgatct gacgcgtctt gttgagcgtc tcaaaattcc
     7921 gatcgcctcg acactgttgg gaaaatcggt cattcgagag gaccatccgc tgtatgtggg
     7981 cgtctatggg ggattgatcg cacgcgatga agttcaacag ttcatcaatg attccgattg
     8041 cctgttgatt ttaggatcga tcttgtccga tctcgaggat ttgaatgccg actccccgtt
     8101 gcggtccgag ggccgcacga tccatgcaac cgccgatcac gttagcattc ggcatcatcg
     8161 ctacgatacg attcgcttcc aggacttcgt ccaggccctg gggacgcatg gtctgtcggc
     8221 tcagtcttct cgtccccttc caccacccct ctcgatcact gggccgccgc ctgcagcgga
     8281 ctcgcaagtg acgttgaaag ggctgttcgg gcatctggat ctaaaattgg accaaaccac
     8341 gcttgtcatt gccgacgtcg gggaatcact gtttgcggca gctgacctgc gtgtgcatcg
     8401 ggggtttgaa tttctctcac ccgcctacta tacctcgatg ggattcgcag ttccggcttc
     8461 gatcggagcg tcgtttgccg atcctacgct ccgtccgatt gtcctggtgg gtgacggcgc
     8521 ctttcagatg acagggtcgg agctgtcgac cgccgtgcgg tatcggcaag cgcccattgt
     8581 cattgttttg aacaatcgag ggtattccac tgaacgcgaa attctggaag gtcccttcaa
     8641 cgacattcat gactggcgct atgaacggat ctgcgatctc atcggcggcg gcatcggcgt
     8701 cgtcgtgcga acgcagcaag aatttgaaca gactttggaa aaggccctgg ccgattccgg
     8761 ccagttatac gtgctaaacg tcctgctcga tccgggcgac cgatcgcccg gcatgatacg
     8821 cctggctcac cgtctggcga aacggctctc taccgatcgc ccataacttc catacgctcc
     8881 gattgggtaa tcgaggtggc acaccgagga ctcacggaga taaaccctct ggcctacggg
     8941 ggaagaaaaa aagcagggct ctcagcatgc gggttctgct agcctttcgg ttgaggtggt
     9001 cctttcgacg caaagaccgt cgcgtcacag gccagatact tgatctgctt catgggcacg
     9061 atgatcactt ccttcgccga atccacttcc aacacttcca tgtcgtcact aatcgtatgc
     9121 ctgacggcat ctttgacgag cagttcctgg ttatccacga agacaatctt cacccaatac
     9181 tgcacgatac ggttctcctt ctaaatagtc gtacctaatt cttgcgaccg ccgcgttgcg
     9241 gcttctactg cggagatcag cgtcgcccgc agaccgcctc gctcgagttc atgcagtcca
     9301 gaaatagtcg tacctccagg cgaagcaacc cggtccttca gcatcccggg atgctcccgt
     9361 aactcgagca ccatccgcgc cgcgccactg acggtttgcg ctgccagaag ctgcgccgtt
     9421 tcacgaggca atcccgcctt gacgcctcca tccgccaacg cttcgatcat gacatacaca
     9481 taagccggtc cgcccccact caggccggtg acggcatcaa gcgccccttc ctcgactttc
     9541 acgacctgtc cgatggattc gaacaacgca cgcacgatac tggcgtgtgc atcggagagg
     9601 cgatcatcgt acgcaatagc ggtgacgcct tctctaatga gggacggcgt attcggcatg
     9661 gaacggacaa tttgggttgt gcccggcacg tagtgcgcaa tgcgggcgat cggatatcct
     9721 gctgcgacag aaatgaggag cgtctcttct cgaatgacca ccccaagatc tgcaagcgcc
     9781 tcatccagca cttgcggctc cactgcgaga accagaacgt ccgcctgttt cgcaacatca
     9841 cgatttgtcg acgccgcttc gatcttgtaa ttttcgcgaa gtcgggcgac cttcgacgca
     9901 ttgatatctg acgcgaggag ctgagccggt gtgattctct ttccatgtaa gaggccggag
     9961 atcagcgctt cggccatatt ccccgcgccg atgaatccta tcgtccggcc agacagcatc
    10021 gcgcaattat actgtcgcgc tttgaagcgc ttcaacttgg aacttgctga ttccttcggc
    10081 tcttcggtac agtaatgtcg gtcgtttttg cgtatttttc tttatccctc acttatcatc
    10141 atcgatgctg gaggcacaca tgaggcaact ggcgctttcg agtctggtgc tcctcgtact
    10201 gcttgtctcg aacagttggg cccgccagga atcgctcacc gcggaagaaa agcagaaact
    10261 cgaaaagatc gatcgagtgc tggttgaggt catcgcgatc tcagacaaag gatcgaccga
    10321 tgccggccct ttaacggacg tcgtggtgaa gcggatgaag gaattcggat ataccgtgat
    10381 gaccgatccg tcccaaccgc atgatgtgac actgaacgtc aaatgcgagc agcgcaagat
    10441 ttgggaaggc acgacgaaaa tgggcagcga tgccgacctg ccggattcac catcgcgctt
    10501 gtggaaaggt ccggcctgtc aactctccta cgtcttagaa ggcaaaaaaa tgccctggcg
    10561 gaaagaggtg agaacggatt ttacggacgc cgaacaagca gctgtagtcg cgaacgccgg
    10621 agacccatct gcgttcgcca tgaacaaatt gaaggaacgt ctggaggatt atgactttcc
    10681 agcgctcatc acggcggaat ggggacagga agaacgtttg ttcaagctct acgatgatcc
    10741 ttcgacttct tccgcccgga aagtgcggct catcggcctg tttggctatc tcttcgagac
    10801 gaaagccgtg ccccgcctcc tcgccggatt gaaagacaag gacatcgaaa tagccaaggc
    10861 ttcggcgttg gcgcttggaa atatcgggca gaaggattca gttccggcct tggttcaagc
    10921 catgacgagc ggtccggccg aactgcggcc gtcagcggcg aaagcgttgg gtgtcttggg
    10981 cgctctgcat ggggatttct cgatcgtcga acccttgctc gaaacgttga agaccgatga
    11041 tgtcgcgttg aaaacggaag tcgcgtgggc tctgggcaaa ctgcctgatc gaaaggccta
    11101 tgaaccgctt tatgcgttac aaaagtccct gcatcaggtt cgcgaccatg acgccgatcc
    11161 caaggtagtc aagttgaaag aggccgtcaa ctggagcatt aagcaaattg acacctggga
    11221 gtaccttcaa taaggcgccg tgaggccttc tttccatgat ccgccgggag gattcgtgaa
    11281 gctgtcgact gtgctcattg gaatcgtctc cttggcatgt atcacagagt cggcgtggac
    11341 gcagacgatg ggagacgagg cggagctgga acgactgcga gtcaaggcgg aagaagcgat
    11401 cgcgagcgac gatcccgaag gtgctgccat gaatatgggg agggccgcgt tgatggccaa
    11461 acggttgggt ctgctcagcc gagacaacca tgcgtccgtc cgtctgtatc agggagcgga
    11521 atcgctgttc cgctcacaag aacacgggta ccgtgctatg gcattgttcc gtcgagctgg
    11581 tggccagttg ccagcctcgt ccggcgtatg tggcagtctc gccctggctc agggcagcgt
    11641 acagcgcgcc ctgttggatc tcagcagcga atcccttcct gtttcactcg tcgaacgtgc
    11701 aacgcgttgg cgggaaactg ccgatgattg ggtcattgtc gtggcggcaa tgattactga
    11761 ctaccaatgt ccctagagcc agctaacccg ttcgacatcg gctaatcctc ctcgggattg
    11821 atgatatgca gaccggcggt tttactcgca gacaagagct gcgtatcggc gctcacaacc
    11881 gtgagtcgga ggggctttag ctccaatgcc aacgctaaat gcaacacctg ctgcgtcctt
    11941 aaaccagggt attccatcat cagttctttc gtagacagat acgtgtgcat ggtaggcacg
    12001 atgaattgga aaagcccttg ggccgattcc cgctcgaatt tatggatgac cgagtagcaa
    12061 tcatcccgtg tcaattcgcc ttgctgagca cggttcgaaa aggccgaata caactccgtg
    12121 acagtccacg tagggaggat agccaccttg cctcgcttga ccatcagttt attaacgatt
    12181 ctcgttcccc gttccatgct gtagcgtttc acgagcgccg tcgaatcgaa ataataatac
    12241 ggcatggatc agaccctccc tttgagaatg accgcagcca atggcccggg catttttgag
    12301 agccggtcct ggagttcctc caagggtact tcttcgtatc cttcacggcg cagtttatcg
    12361 gccagattgc cttcattaaa agctgccgta tcactcttta agcgctcgtt cagccgccgc
    12421 ttcgccagcc ggctgggaac acgttgttgc gtcgccgtca atcccctgcc gcgactgcgg
    12481 ggcgcccgac ctcctggttt cgatctgctc atgccggtct tctccctccc tttcgagtta
    12541 gtgagttgca tttgccggtc tggctcttaa cgcctcccgt ggacacccgg ctgggcgatt
    12601 cgccgcaccc ggcaattcgg actctccata cacatgtgcg gcgatggccc tggcgacatc
    12661 cgccgacagg tcctgctgca tgactctgat cacttttgcc gtggcttccc gctcggataa
    12721 atgcccttcc tttccacctt tcgaaatcgc cccgatgatt tgctgtcccg ccaggtcaac
    12781 gatttcaaaa tcgctgcacc agcgaacata ggtgaactgt gggtcacgaa tgtccacagg
    12841 ccagacacgc accatcccgc gaatcagtaa ttccggtgtt aaaggaggtg ctgctccgct
    12901 cgctgtttcc gttcctgatg ggacggcggc aacgcggagt ccttctttga tcaacccttc
    12961 tgtcaatgcc cgctgcactg gttcgacatg atcgcctgtg atggccacgg tgaggacgag
    13021 atgtgcggcg aggtactctt ccaactcgcg gcttaattcc tgaagacgaa cgcgcggtgg
    13081 catgccctgt ccgctggtcc gaacgacacg aagatccgca ttgtaagttt cgcgcagggc
    13141 cacattacgg gtcgcccgtc tcaaggcgcg cactttcgcc agccggtctg acgtgcggcc
    13201 ggcctcatcg acgtcggaca ggacggcacg atctaactcg gtcatgcgct cgatcaacgc
    13261 cgtttccgcc tgggcccgat gcatgccgcc caatgcgtag tgcagccctt ttttcgcatc
    13321 gtaccagcgg tcgagaattt tcgcattttc caacaccttg tcggtggaga cacgcgtcaa
    13381 atggtcgagc gtgagacgtc gctcgtcatg actggtccca tgctgctcga caaccaagta
    13441 cgattcccag tcttgcgact gggcggaaat ttccgctttg aagatacgag ccaatgcggc
    13501 gtaggcctga tcgtcggcca cggtccgatt gtcgccctgt cctacgcctg tgagatattg
    13561 cgccgaggga tactccgaat tggcaccgtc catccaggaa ggctttgagg gtcctcccca
    13621 cccggaacat cccacgagaa gcgccgtgaa cacgatcgct gtatgtctag ccctcaatgg
    13681 ccaggctgga ttcaatgcat cacccgttgg atgaggatga ccgtttcccc tgcctgaagc
    13741 gacaaggttt tctccgatga cgctggcgtc atcaatcgaa aacctacatc ggcaaccgcc
    13801 ccttgtacgc ggtattcgcc gggagatacc caggcccggg cgaggtgaat ttcatccggc
    13861 agggttcgcc agctgcgctt gtccgattct tcggaggcca ccgcaaatcc cttggtcaga
    13921 agcccgacgg ccagtccgac ccagggtgcc gcatctcgtc ccgctgcctg ctgcgcacct
    13981 ctcgtggcag cttcggccat gctgaacttc agcgcggcac gggccacggc cttcaccgtg
    14041 atgccaggga gacgctcagc cagcgcctta ctggctaagg cggtgacgtt ctgcaccaac
    14101 tcggttcgca ccatcagggg agggccatca gccggcagca gtcggatggt gtctgtacgc
    14161 acttgcgttt tttgcgggac catcatcggt aacgccacac gaacgacccg gccgttgagc
    14221 ccgtataacg cggtatcagc cgctcgatgt tgctggcggt tcccttggaa gagccctcga
    14281 ttgagcagca ccagttggag cgcgtcaaga ctgatcggta cgtcaagaaa ctggtcctcc
    14341 ttacgcgggg cgcggccatt atagctgatc acaatcactt gagcgagctg ttgctgggtc
    14401 tcaaggctcg tccaagccgt atcgggaaag cgccgctgat agtctgagaa ttcctggctc
    14461 aaatgcaggg cgtccgccgt tcgcaagagg tcagcctgca attgggccgg cacccctgtg
    14521 cgcgaccatc cgcgagagga gtcaaacatc tcatacgcct tgcggtacgc gatgaacgca
    14581 ttattgacgt ctccggtgct ctcgtagagg ataccgctga ggtaccgtgc aaacccgtcg
    14641 tcatgatagg cggccttgtc cttggttcgg tcggcgagga cattcagccg atggtccata
    14701 cggcgggcct caaccagcgc atcctgccac cggttcagcg acgcatagtt caaggccttg
    14761 aggacattga tcatcacctg ttcatagggc tccccctcat acggcaaggt attatcgttc
    14821 gtcatgaacg cagcggtttc cgtacgaacc gttctcgtat acagacgatc cacttcctct
    14881 tcagcttcct ccaggacgcg gttgctctgt tcgtattcgc cggccagctg aagggtcatt
    14941 ccccggtcca tgccatacaa cacacggctc tttgcgccgt agtccttctc cgcctgccgg
    15001 atgatagcgt ctgcttcctg gtaatcgctg gtgagaagcc ttttatcgac gagaagataa
    15061 tggctctggg aaaaggtgca gccgccaagc gcggcagtgc agaggaaggt cagtacgtga
    15121 gcagacacac aaacgcccca ccacgtagag gcgtgagcgg tggggcgtga gacaagaggg
    15181 ctcaacggag tttcgcggag ccttagaaaa ttgttcgctt cttctcgatg actttcttga
    15241 tcttcttttg tccgaaccac accttggcgt tgctttccaa atccaccatc tggagatcga
    15301 cctgatagaa catcgccttg gtgccatctg cttcatcaag aatcgtggcg atcgtgccct
    15361 tcatgatgta atcggcaccg atttcccggc cgggtgcctt ctgcgtatcc tccctggcat
    15421 acatcgcttg ttctttgcgc tccgaacgga cctcgtcccg ctcattctta cccgccacga
    15481 acgtgacttt ttgagaattg gtcagctcac gctccaaatc ggtgacaaac gtgtgcacgt
    15541 tgatgtgttc atgactactg ttcaacacgg tgccgacgac gaccaccggc tgccggcgtt
    15601 tggactggct aaaggtattc agccacggat actcgagcgc ttccttgacc atggattcgg
    15661 ccactatacg agagtccgta tcgttccacc gcccactgag gtcggtcacc accccggcat
    15721 cgatacgggt gaccctggtt tcacccccac acgccgacaa acccaccagc aggcacatga
    15781 gcaccgccgg cttggtccat tggatgcgtt gcgtcatgat gctcatcacg ggctccttca
    15841 tgcgtccggc ttaatggttg gcccgcttgt cttcttcctt ctccagacgc tcgaacagct
    15901 tgtccgcatt tttacggacg aaatctcgca cctgggcgtt taactcctta gcctgctcga
    15961 gattgttctt catttcctcg agattcagtt tagtcaacac gtagtaggtg ctggtcttct
    16021 cgtctttgta tcgctcaatc ggacgcaccc cgctcaaggt ggtggtggtc acggtcttga
    16081 tggctcgttc gacgttctgt tcttccgtgt tgcgcgtaaa atcgccggcg gttgtcgatg
    16141 ccgcataatc gcgcatcaga tagccggtgt acgtttcaaa ggtcttggct atttcggcgc
    16201 gggcgcggtt ttcagcagtg tcccaggcca atggctcatt cttgacgccg gtgacggatc
    16261 cgacgccata gaaggctttc gtatctttct cattgaaggc accggaccct tgcttcaccc
    16321 atttcggagg gccaccgcat gcggtgagcg ccagcaacag gatgagggcc attccaccac
    16381 aggtcacgtt tgagaaacag cctcgtggct tcatgcgatg tcctccttaa aagtgatcat
    16441 gtctacgcac ctccggagcg ggattggctt cgccaactgg tcgagaaatc gagcgaaatc
    16501 atgctaacat gcacccctag tccagtcaac gcgatcgaca ctttcatgcg tacgttcatc
    16561 tttgaaggag gactttttgt ggctgacgtg cagatcgacg acggcatcat caagatactg
    16621 aatctcgacg tacaagatcc gaaagcggcc gccgtgctcg ccgaatatcc gcaggtccgg
    16681 tgggcggaaa tcaccagacg agccttgaaa atcggcttgg gctatctcaa gggaggcgca
    16741 cgagattagg gggttttgga ggaaggttgc gtgtctgttg cccgcgactg cgcgacgact
    16801 tcacgtgccc gattatgagt gccgcccaat tcgagatacc gctcaaaggc ttcgaccgct
    16861 ttcttttgat ccttcaagtg atcggcatac agggacccaa gcgtgaagta gacgtcgaca
    16921 taactcggat tggcggccaa cgctctccgc atggcctctt cggcctccgc atactgtccg
    16981 cgtttctcgt agatcagtcc gagcgtatag tgtgaatccg ggttgtttgg tgcatactga
    17041 acggctaact ttccggcctt cagcgcttcc tccagatcgg gaatcacctc cagccgcacg
    17101 tagcttttga gcacataggc gtcgccaaat gtcggatcgt attccagcga cttgttgagg
    17161 cgctccaagg cttcgtcctg cgcttttcca ttttgcagga gggcaaaagc ctgctcgtat
    17221 tgcatcctgg ctgcttgctg acgttcagcg ggtgactggg gctccccacc cagccgaaac
    17281 gccgcctttc gtccttcgat gatgatggta tcgccatcgc cttccagctg ggcaaacgtc
    17341 ggatgctggg cacccttcgt gtcatccgtc acctgttgac tgatgaaccg gccgacctcg
    17401 gacgccatca gccaaccatt tttgtctatg tccccctccc cggtgagcgc tttgatcagc
    17461 gccagtacga acacactgcg atcctgtgtt ctcaccgcgg cctctccttt gtcggccgct
    17521 gtcaacacct ggacggcacg tttttccgta tcgtcctctg gggcacttcg cccttctagc
    17581 gaaagcgcct gagccgcact ggcctcccac cctcggacgg cggtatcgaa tacgaatagc
    17641 gtgtgcttcg aggcgctgcg gtgactgaac tctttgagct gttcaaaggt cacggccttc
    17701 gagacattcc cgggctgcgc gtcccagggt acgagataac ccagatcctt ccccgtgccg
    17761 ctctgagtaa tcccagcatg gccggagaaa taaatcacga gacgatcctg acggcccacc
    17821 ttccgaggca gaaaatccac cagtgtctgc tgcaatcgcc gcgaacttgc gtccttgtcg
    17881 tagagttcga cgacctcttc gaatcctaat ttgcgaaagg cttcggcaac ggctttggcg
    17941 tcttcaatga caccgggaat tttcggcgca agcaggtagt tttcaatacc aatgacgacg
    18001 gcccacgatt tgtaatacag tccctccggc tttcccatct gagcgagcgc cgcaacgggg
    18061 ctgaggcctg tcatagcgag aacgaccaga atcgcgagaa accgtgactg gagcgatggg
    18121 ggtgaggtgg gaagcatcat agagggcacg tctcaagccc tgtgtgaaat cagcgtactc
    18181 tggccctgtg cggctgtcaa ccacgggccg gaggcaattg catcggcgac cgtcccgttg
    18241 tatggttgcg ccgccccaac cgcttgtcga taatggcggt atccggtacg ggccatccca
    18301 aaggagctgt tcagccatga cggagaacat tgaaaccctc ctcaaagaga gccgtgtcta
    18361 tcggccgacg gccaagacca tcgccgcggc gcacatcaag gactacgaga aagagtacca
    18421 gaaatcaatt gccgatcctg aagccttttg gagcgggatg gcgaaggagt tggactggtt
    18481 ttcgccctgg tccaccgttc tggaatggaa ttacccctgg gcaaaatggt tcgtgggagc
    18541 cacgtgtaat atcacataca attgcctcga ccgacacgtc aaaacctggc gcaagaataa
    18601 ggttgcactt atctgggtcg gcgaagacga ccgtgaacgg atctttacct acggcgaact
    18661 ctatcgccag gtcaatcgat gcgccaacgc gctgaagaag ctggggctga acacaggcga
    18721 ccgcgtcacc atttatctcc cgaaaattcc ggaacagctc attgccatgc tggcatgcgc
    18781 ccgcatcggg gtgatccaca gcgtggtgta ttcgggattc agcgcgcccg ctctggccag
    18841 tcgaatcaac gacgctgaat ccaaattggt catcaccgcg gacgtcggtt tcgaccggac
    18901 caaggtcatt cccctgaaac cgatcgttga cgacgcgttg aagcagtgct cttccgttga
    18961 gcgggtcgtc gtggtcagac gacaaccctc aagccccctg ccaaccgctc caaaagaact
    19021 cgactggacg gattgggtca aaggtgaacc ggctgtctgc gaagcgaagc cacttgactc
    19081 agaaactccc ttgtacattc tgtacacgtc cggcacgaca ggaaaaccca agggggtcgt
    19141 acacgtccac ggcgggtaca tggtcggcac ctacacgacg accaagtacg tgttcgacat
    19201 gaaagacgac gacgtgtact tctgcgtcgc tgatccggga tgggttaccg gccacagtta
    19261 tattgtctat ggcccgcttt taaatggcgc taccgtgctc accgccgaag ggaaaccgga
    19321 ttttcccaat ccaggccggt ggtgggatct gatcgaacgg tatggcgtct ctatttttta
    19381 cacgaccccc actgccattc ggctgctgat gcgccatggg gaggactggc caaaaaaata
    19441 cgatctatcc agcttgcgca ttcttggcag cgtcggtgaa ccgattaatc ccgaggcgtg
    19501 ggaatggttt catcgtgccg caggcggcga taagccgatt atggatacct ggtggcagac
    19561 ggaaacagga tccattctga ttacgccgtt gcctaccgtt gccttgaagc ctgggtccgc
    19621 cacgagacca ttcttaggga tcgaagccga cgtggtggac cgccaaggca atcccttgcc
    19681 cgcaaaccag ggcgggttcg ccgtgatcaa gaaaccgtgg ccatcaatga tgcgtaccat
    19741 ctataaagat cccgaacggt acaaaaccta ctggaacatc atcccaaact gctatacggc
    19801 gggcgacgtc tgtcacaaag acgctgacgg gtacatgtgg ttcatgggcc gtgccgacga
    19861 tgtgatcaag gtcgcgggca atcgcctggg aaccgctgag gtcgaaagcg cgctcgtcag
    19921 ccaccacgcg gtggctgagg cagccgtcat cgggataccc cacaagacag tcggtgagac
    19981 gatcaaggcg ttcatcatcc tcaagcaggg cgaaactgca aaccctgcgc ttatcgagtc
    20041 cataaaagag caggtccggc atgagctggg gaaaatcgcc gttccctcgg aagtcgacat
    20101 tgtcacgtcg ctgccgaaga cgcgttccgg caagatcatg cgtcgagtgc tgaaagcgaa
    20161 ggaactcggt caagatccag gagacatctc aactattgag gaataatgca ccgcaagttc
    20221 atgcagcgcc ggtggcgctg cggcgaagga ggcaggcatg gtgaagcgaa tgggccggac
    20281 gatgatgttg attgtggcag tcggtttcct gctcgtaacg ggggaccctg ggctggcacc
    20341 agcggcggac aaggaaaaac cggccacgaa gcccaaagct gcccccgaag ccaaggcacc
    20401 caaacccaaa gcccccaaga ccgacaaacc gaaatttcaa acggctgaga aagcaggagc
    20461 agcgaaagcc tgttttggcg aagcaccaag aatcaccaaa ctgacccccg acgaagggaa
    20521 agccggagac aaagtcacga ttaccgggac gaattttggt tcggccgatt gtctgcgcgg
    20581 tgtctctttt ggtcccgggc atgcggcgac cttcaaagtg aaggacgaga ccacgatcac
    20641 cgccactgtt ccgactgcaa ggcgaaaagg gctggctatc gtcacggtga cgacggcatc
    20701 aggagaggat tcaaaagcgt ttctgatgaa atgaaattcg gtctgatcgc gctgcttcgc
    20761 ttgaagccag atccttgttg gtcctccgta agcctcgcaa tcgcttggcg tcgcaacttg
    20821 ttccatcctg agcttcgctc gatggaacgg cgaacagcgc caccacccgt ctgagacgga
    20881 acgctccctc tcggtaacgg aagacacaac gcggctctga tgctcacttc agcggcgcgg
    20941 ccagacctct gatggatgga caataaaccc ttaaatctga aagaaagtag cacgatgcaa
    21001 gggggacgga gaaagccggg cgaacccatt tatctgagac ggcacgtgat cgctctggcc
    21061 ttagcggttg gtttgccgat cattgctcta cagatctaca agatctatgt aggccccctt
    21121 cacttcatcg tccaaattgg cctggcagcc ttcgcttcag tggtggctgg gctctggctg
    21181 tatgtgagct accggtcctc ggcacagaat gaaccatgaa tgagtgcgaa cacgctgcct
    21241 caatggatcg cgtacggaca ccacccaggc ctagcgagcc ttcacggtac gaaacgtaat
    21301 ggagtagcgt aactcctttg cgggcggtat ggaatgctgc cacaggaaac gggcttcctc
    21361 ggctaaaaga tatgccgacc tcggcgcgag gattgcctcg gccgtttctc gttcgccggc
    21421 gactttgcgc tgaaaacgca tacggcaagt ccctgccaga gagacaccga cgaccatcgg
    21481 accgaacatc ggggcatctc gatgccatcc gatgccggca cccggctcat agcgagtgac
    21541 taacacttct tccaccgctt ctgccgggac gcccatcaga gcaccggccc gcaggcgcaa
    21601 aggcatcagc caatccggga tcggctccgt cggctcgatt gtccacgaat cgtagccgta
    21661 atcccacccg aaatggacaa cccgtcgctt cgcggtgacg ccatgcatgc ggacctccga
    21721 gaacgacagc ctttcgatct gcgacaaaag agcattctcc tcttccactg tgatccaatc
    21781 aggcaggtag tgaaaccctg gcggtggtgt agaggagaat aaggagcagc gcatggacca
    21841 ttgtatcgag cctccagggt ggagtggtat gggcgggaaa cgccagagaa ctcaggctgc
    21901 tacaaaagtc cgcgcttttt caggtagtcc ttgtcaatag cgggggtcat ctttggtagc
    21961 tgacttcttt gctgaagcag ccgctcgacg tatttgccga tgaggtctga ttcgaggttg
    22021 acggtgtcat tgacctgctt caatcccagc gtcgtggctg tcgccgtatg gggaatgacg
    22081 gcgacgctga acgaagagtc ggtcacatgg ttaatcgtaa ggctgatccc atcgactgtg
    22141 accgaaccct tagagacgca atagcgcaac acatcaggcg gcgcttcgat ggacaagacg
    22201 attgcattcc cttcctgatg ccggctgcgg atagtcccca ccgcgtccac atgacctgcc
    22261 acaaggtgac cgccgatccg ttcattcaac ttcatggccc gctcgagatt gaccggcgtc
    22321 cctccgacca gttcaccgag ggtcgtcacg gacaaggtct cgggggaaac gtccaccaca
    22381 aacgttgttt cccctttagc caccactgtc aaacaggtcc cgttgatgct gacactgtcc
    22441 ccgaccttca gatctcccag gacggccgag gccaggatcg tcagtcgtgt gccggcgaga
    22501 gttttttcaa tcgcagtcac ggcgcccatc tcttcgacga ttcctgtaaa catacgagtt
    22561 attgaaagtt ctcgtctctc gtctttgcca tttgtgagaa gagaaccagt gagcctattc
    22621 gcctttctga gtaaacattt gcttcaccac atagacaaac accacgatca ggaccatgac
    22681 gccgatcgac gcgagcacac cgatcacaat gttttcagtc gcgatttccc gcatggcact
    22741 ccattatagc ggtatttatt ttctcatggt gagccaaaag aatccggcgg cagcaacctg
    22801 gaggccggca atggtcgtga acgccgaggc ataactcgtg tgggcaatga gtacaccggc
    22861 cagcaatgga ccactggcat ggccgatatc tccgatggtg ccttgcattc ccatgcctgc
    22921 gcccagcgtc ttgaactccg agctgtccgc cacgaacgcc gaggaagagg acgagaccac
    22981 cgcctccccg aaaccgaacc cggccgtcag aagcaccaac acggcaaaac tcgccacatg
    23041 cggaatacag acaaacgtga cggcacaaat taccaagccc aacatgatca atggctgcct
    23101 gccgactcga tctgagactc ggcccatgat gggttttgag atgaacgacg tcagggattg
    23161 caccgtgaac agaagtccga cttcgccagc gttgaggccg actccaactc cgtagagcgg
    23221 caaaaaggcc atcaacgctc cattggcgat cattttggcc gcatccgtgg agctcgtaat
    23281 caggactttc cggttgcggg cgacgatact gaaccccttc cacatttccg tcaccaccgg
    23341 cgccaggccc ttttcctgat tgcgtagcgg aggcggggtc agccgtaaac tatagaatag
    23401 gacgatagcg atgcacccga aaaacccggc agtgagaaac gcagaggaga agccggacgc
    23461 aaaggcgagg tatccgccga tgaacggacc caacaaggct ccggattggg tacaggccgt
    23521 ataggtgccg agcgctgcgc cgcgccgctc gcgatacagt tcggcgacgg tggcgagcgc
    23581 gctcggggcg aaaatcgccg tcgccaagcc gtgaaaaagc cgtagcaatg tgagcagatt
    23641 caaatccgaa atgaagggat agagaaatgg aggcagaccg aacgcgatga ccccgacgcg
    23701 cagcagtatg cgacggccgt aaatgtctga aagagcaccg gaaggaagct tgagcaacac
    23761 acctgtcaga gtggacactg acacgatcac gccgattcgc tcggggctgg caccaagcga
    23821 ctcagcaaac aacgccagga ccggcatccg taccatgtta tagctgataa aacaaaacac
    23881 gccgatcgtg cagaggagcg taaaactgcg agcggtcgtc atggcgccgg acgcgaggag
    23941 tttggagtgg ccgatgaagt gggccggcgg cttagggcct gcgttaagaa cgcgacgtcc
    24001 tgcttgggca agggaggcgg atccgtagcc tcgaacagca cgcggtcagg ccgttgcccc
    24061 atttcgagac ccaaccggtt caccacttcg acgcctttag acaatttacg cattaccgtg
    24121 gcatcgacga gcgggcgcag aagcgaaacg aagccggaaa gaacacggtt atccaacatc
    24181 gtgtacgcca ccatagtgct ttccattgca tccgagccgc gctcgtcctt cactggcctc
    24241 atccgaagaa agatcacggc cttgccgctt acattgggaa gcaagcggct atggtgcgtg
    24301 ccttcaaggt aatatacccg cgtcgcgcga tcctggtata ccagctgcac gatgccctcc
    24361 gtaccttccc catcgctgcc ccaccaccgc ccaggtcccc gctcctcgga cttgtagtct
    24421 gccagatcca gccgattgat gagtgccgcc gctaccggtg gccggtcgag caggtacaga
    24481 tacagtggct cagccatcac ggttttcacg ggtccaacgg tgttggcact gaggtggtgg
    24541 tcaatgacga attgaaggcg gcaagtccag tcaccggcaa tcttcccaac gggaaatgca
    24601 attcccgact gttcactggc cacaaaacag gactccgccc gcgcgcgacc gagagaaatc
    24661 gatccgagca gcgcaagtat gagccagaaa aatcttgacc ggtttccgtg caacagccgg
    24721 tccccgatgt tcatgaggcc tgatttggaa ccaacacgga gagatgaatg ggaaacagct
    24781 gccggggatc gtgacggagc cgtgcttgac gtaacagtat gtcaattgac accgtcacgg
    24841 gaccatcata ggagagttgt ccgttcgtcc agatgctgac cggttttgcc gtgtcgataa
    24901 gacgatcgtt caaaaagaga ctatagcgct gtatgtgttt cgtactcacc tcgatacgat
    24961 tcggcccgac gatggccgca tcgagcttcg cgtattcgcg ttgtttgatt cgctggtcgc
    25021 gcttgtcgat cagatcatcc gaaaacgccg cgatcacgtc cgtggcatca acgcgcaccc
    25081 agctgaaagg ttggaagcgg ctggcttcgc gcacgagggc caatttcgtc ggcgcagggt
    25141 cacggcgctg acggtccaac catgtcctca atgccggcaa ctcttctctg ggaaaatagt
    25201 ggccgcccgc gaccggatgc tctccctggt gctcgcggta gacatgtgag taccccaatg
    25261 ccgtgagttc gcgggagatc gaccggctca atgacaccgg catgacttga tccttctccc
    25321 catgaatcat gtagacgggg gtattgcgaa gattggcgag gaacggcatg agcacgtcat
    25381 caagcccgcc ggccatcggc gcaacaccgg caaagagcgg cgcatggtgc atgccaatga
    25441 gccaggcccc gatcccgcca ttcgacatgc ctgtaaggaa aatgcgatcg gggtcaacat
    25501 gataccgccg acgaacgtgc tcgatcgtcg ccagcacgac ttcctctgca cgccgggtaa
    25561 accaggctcc acttggatac gtcggacaag ccagaagata ctcttcgcca agccgtgcct
    25621 gccaacgttc cagatagacg tcgccggtaa accctgcgcc gtgcaggcac acgacgaggg
    25681 cataaccatt cgcggatcga tatgtcaacg gaatcgagag cgcaaccgcg taggtttgac
    25741 cctcgactcg caccccttcg ttggctaccg tgccgatcgg ctgggcgtca taggtgcgtt
    25801 cggtttcaat gatccgggtg acgcgctcga cagagatgtc aggatgattg agcaacacct
    25861 tcaagaaccg ttcagcctct tcgctgtctg ctgaatcgag atagtgaaag accagtgtag
    25921 agaggctttg cggcggatcc acacgagcca tcgtcaagct cgtttccaag ccgacagcca
    25981 gcgcaagaac gcctacggcg gccagccatg gcctcataag tcaccttcga ccacaatgtc
    26041 gccgccgagc catctcgtac gcacatgctt gagcttcaag gtttcggcga gagtggctgg
    26101 actttccccc ccgatcaccc cctttgcatt gctgccgcca agcagtgcag gcgccatgta
    26161 taggcgaagg tgatggacca gcttggcccg caacatggcc gcattgattt cactccctcc
    26221 ctccacgagc aacgacgtca ctccccgcct ccccagttcc ctcatcaagg cgggtaagga
    26281 gacacgtcct cgaagactcg gcagaacgac gacctccacc ccgagcttcg tgagggcgtg
    26341 ccttcttgcc gcaggagcgc tgcgagtggt ggcaatgagt gtcttggcga catgctgctg
    26401 tttgagaatg tgcgctttga agggaatccg aagtcgactg tcggcgacga cgcgcagcgg
    26461 ttgctccgac gtcaatttct tgaaacgagg gcccacacgg gcggtcaatg atgggtcgtc
    26521 gttcacaaca gtcccgattc cgaccaacac cgcatcgact tccgagcgaa gccggtggac
    26581 gtcgcttcgc gacggcacac tcgtaatcca cttcgcctcg ccggacgccg tagcaatctg
    26641 cccgtcgagt gtcattcccg ctttgaggag cacataggga cgcctcgtgc gcatgtaatg
    26701 attatacgca cgattcaact caatcgcctc ggactttccc acgccgaccg tgacggatat
    26761 gccggcacgc cgaagcgcct gcagagcttt cccgttgacc agaggattgg gatcgcgctg
    26821 ggcaatcacc acgcgtgtca cgcccgccca tatgacggcc ggcacacagg gaggtgtccg
    26881 tttattgaga tgacagcagg gttcgagcgt cacgaacagg gtgccgccac gggcctgagg
    26941 ccccgccgct ttcagcgcca gaatttctgc gtggggaaga ccgggccgga gatgatatcc
    27001 cttgccgacg atccgtccat ggctcacgac gacggctccg accatcggat ttgggctcgc
    27061 cgtgcctcgg ccattggccg ccagggcgag cgcgagtctc atataatgaa gatcctgtgc
    27121 gtggccagcc cgcattatcc acgaggcctc gtcacggtat gcgagatcgg gagagcaata
    27181 gcccaatcat gaagccgatc gttgagcagc ataggagccg cggtgggaaa caccttcatt
    27241 gagccatcga ctaccgcttc ttgcgcctcg cggtcgactg ttttcgcccc gtcgccttcc
    27301 ggagcgtcgc ctttgcagct ttgcgggcat ccgcctcagc caatgcggca tgtgctgcgg
    27361 caagccgcgc gatggcgaca cgatatggcg aacaactgac ataatccaac ccgagctggt
    27421 ggcagaactc gaccgaactg ggatccccgc catgttcccc acagatgcca agcttgatgc
    27481 cggcgcgcgt cttgcggcca cccgcaatgg cttgactcat gaggcagccg acaccttcac
    27541 ggtccagcac cgcaaaagga tcggattcca tgaggttcac gctcttataa tgatcgataa
    27601 acttggccgc gtcgtcgcgt gagaatccga atgtcgtttg agtcaggtcg ttcgtaccga
    27661 acgagaagaa ctcagcttct tcggcgatcc ggtcagcggt cacggcggca cgtggcaatt
    27721 cgatcatcgt tccgaccagg taggacagtt tgacgttgta tcgcttcatc gtctcctggg
    27781 cgacctcacg aacgagatcc ttttgagatt tcatttcgga aaccattccc accaagggaa
    27841 tcatgatctc gggcacaatt ttctttccct ccttggccaa ttcgcaggcc gcttcgatga
    27901 cggcccgcgc ctgcatgcgc gtaatctccg gcatcgtaat gccaaggcga cagcctcgca
    27961 aacccaacat gggattgaac tcgtgcaact cctccacacg cgcgaggagt cggcggtttt
    28021 cctccagctt cgcagcgtcc ctgccggtca gctctagctg ggcaatttcc accatcaggt
    28081 cctcgcgctt ggggagaaac tcatggagcg gtggatcgag caaccgaatc gtcaccggaa
    28141 aaccctccat ctcccggtac aggccgatga aatcctgttt ttgaagcggc agcagttggt
    28201 ccagatactt ttcccgctcc tccgtggtcc gcgccagaat cattttctgc ataaggggaa
    28261 tccggtcttc ggcaaagaac atgtgctcag tccggcataa tcctattcct tccgctccga
    28321 agccgcgggc tatcttcgcc tgctccggca catccgcatt ggcccgcacg cgcaagcgac
    28381 gaaggctgtc cgcccaggaa aggatcgtgg agaagcgctg atatttttcc gatttcttgg
    28441 gatccaactt gccttggatc acttgaatga tttcggaatc caccaccgga atatcccctt
    28501 catagacgtt ccccgtggag ccgttgatgg agagataatc gccttctcgg aacgttttcg
    28561 cgccgatctt cacagtttgg ctgtcgagca cttccaccga gtcacacccg gcgacgcaaa
    28621 ccttgcccat ctggcgcgcc acgaccgcgg cgtgggacgt catgccgccg cgggcggtca
    28681 agaagccgcc ggccgcattc atgccatgaa tgtcgtccgg acttgtttcc tggcggacca
    28741 acacgacccg actgccggcg gccttcatat caacggcccg atcgggcgtc agggcgattt
    28801 tccccgccgc cgcgccaggg cctgctggca agcccttccc aagcggattc gccttcgact
    28861 cttccttcgt gtcgaagatg ggatacatat actgcgccag ctgatccggc cccacgcgct
    28921 gaacggcttc ttccctggaa atggcgcctt ccttcaccat gtcgaccgca atcttgacgg
    28981 cggcgatgcc ggtgcgcttt ccaacgcggg tctgtagcat gtagagcttg ccctcctgga
    29041 ttgtgaactc caggtcgagc atgtcgcggt agtgcttctc gagcttcttg taggtctgct
    29101 cgagttcccg atacgcctcc gggacgtttt tggccaatgc gttgacgggc agcggtgttc
    29161 tgattccggc cacgacatct tccccttggg cgttcatgag gcattcgccg aaaaaggcct
    29221 tgtccccaga gcttgggctg cgcgtaaacg ctaccccggt tccactggtt tctcccatgt
    29281 tgccgaacac catcgcgacc acattgaccg ccgttcccca ggaatccgga ataccataga
    29341 gccgtcgata ggtgatcgct cgggcgccga accaggacga gaagacggca ttgatggcca
    29401 tgcgcagctg ctcaagcggt tcatcaggaa aatctttctt ggtctcttcc ttgaccagcg
    29461 ccttgaagct ggcgactaac tcgcgcagat ggcgggcgtc gagatgggtt tcatgcgtca
    29521 ctcccacttc ggctttcttg tgtttcaaaa tggcttcgaa gtgttcacgg ggcacgccca
    29581 tcacgatgct gccgaacatg gacacgaacc ggcggtagct atcctgcgcg aaccgatcgt
    29641 tcctggtttt ggcagccaag ccttcgaccg tcttcgtggt cagtccgacg ttcaacactg
    29701 tatccatcat gcctggcatc gacgcacggg ctcccgaccg cactgagacc agcagcggcc
    29761 gttcgggatc gccaaaaccc atgcccattg accgttccac acgcttgagc gcttgtaggg
    29821 acgcttccca catgccgggc ggatatttct tgccgtgctt gtaatactcg acacaggctt
    29881 ccgttgagat ggtgaagccg ggaggaaccg agatgccgag gttggtcatc tcagccaacc
    29941 cggcgccttt gcctcccagc aactccttca tattcgaggt gccttcggcc tttccgtctc
    30001 caaagtagta tacgtatttc ttagccacga tcccctctcc ttccttcacc cgtcagtgaa
    30061 ggcccgacga actgcgagag tccgccggtc caatctggag ccgatagcgg ctcacgagat
    30121 atcgtctcgc ccacatgccg aataacaaca ccacaccgac gaacagggcc aaccccagca
    30181 aacgaaagtc cggcctgacg cctcgatcca cgtagtattg gccgtcgggc cctcgcttta
    30241 cagcgatgtc tttggtcaac tgatgcggtt taccggatgg atcagccagt ttgatccatt
    30301 cggaacacag ccgcaccgga tctgccgcgc ccttgacacg ctcccaactc aatcgaaggc
    30361 aaacatcctc tttcgcatcg aacagatcgg gcgtcttcac taactcgtcg aggttgacgc
    30421 cgctcaccca gactccgaac aagagcagca cattacagaa gacgatcaac acggccgtga
    30481 ccacgctggc aaaccgtcgg attttcgcct cacgctcctc ttcgaggtcg gactcagatg
    30541 aggcgatcat ccttgacctt catcttccag ggggaggagc cggctggtgt ccacctgcac
    30601 cagcgaaggg ccgcggctgc gttcttctag cctccttgta ccacaatccg cgaaaagtcc
    30661 gcgaacgacg taaaacagtc gctgacctct ttcaaaagcg ataaccggtt acttctcagg
    30721 gccgcatcat ctacattcac catgacgccc tcaaagaacg catcgatcgc cggtttgaga
//
LOCUS       DASSCQ010000043        51062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1511366, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000043 DASSCQ010000000
VERSION     DASSCQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 51062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51062
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511366"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFH05_03145"
     CDS             complement(<1..329)
                     /locus_tag="VFH05_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET6677235.1"
                     /translation="MRPYLLMTIAVLTAACALGPDYERPGADSEDRFRMAEAGADAPS
                     MANLPWWELLKDEQLQHLVRTALAENRNLQKAAATVEEFQARAWVAKSDFLPNMTASG
                     NAPSFGR"
     gene            complement(331..3495)
                     /locus_tag="VFH05_03150"
     CDS             complement(331..3495)
                     /locus_tag="VFH05_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HET6677236.1"
                     /translation="MTSHVFIDRPIMASVVSLVIVVMGLLSVQFLPVAQFPEITPPVV
                     QIDADYPGASAEVVADSVARPIEVQLPGIDNLVYFDSTSSNDGHVTIKLTFEIGTDPD
                     IAQVQTQNREKLAEPQLPTEVIRQGVSVKKMSPDLVAVIALKSTDKNHDAVFLSNYAI
                     LRLVDNLRRVKGVGDAMVFGQQNYSMRIILNPLMMARLSLTPTDIAAIIREQNRDYPA
                     GTIGREPALKGTELTIPVITKGRLTEIKDFEELIVRALPDGSNVRLKDVAQIELGAQS
                     YALEGRWNSSPTTFLLTFLAPGANALDTVRRTRAEMDEMAKNFPAGVSYDIPYDTTKF
                     IEVSINEVVKTLVEAMILVVFVVYVFLQSWRATIIPTVAVPVSLIGTFAGLYALGFSI
                     NTITLFGMVLAIGIVVDDAIVVVENVERHMREDRVSPKEAAKRAMNEVTAPVIAIVLV
                     LVSVFVPVGFIGGITGALYKQFAATIAISVTVSGFVALTLSPALCAMVLVPRPERRGG
                     FWALFDRIFGRTQHGYTSMVAKMVARPVRVMLVFGVVLVLAYGMFATLPRSFLPEEDQ
                     GYFIVVVQLPDGASKQRTDAVLDRIERFFQGNPAVHSTDALSGQNFVFGTRGPNAATM
                     FVPLHLWDERKQPQSHVQALIGMAYGEFAKIPEALLLAFNAPSLRGVGVVGGFSAQLQ
                     DPSGGDFKKFAAVAQEFVERAKKEPAIGQVGTNFRVSAPRLYANVNRERAKALGIPIS
                     DIFDTLQAYFGTFYINDFLKFGRIYHVQTEADPHFRSTPQDISKIYVRAQTAQGANMI
                     PLDTVVTSEYQSGPDPVNHFNGYNTALVLGAAKPGYSSGEALEALERVAKEVLDPKGY
                     AIDWSNISFQERRVGGQSSQVFLIGLLMVFLVLAAQFESWAVPFAVILAVPFAIFGAL
                     AAVWVRGFTNDIYFQIGMVTLIGLSAKNAILIVEFANTRYEAGKPLIEAAIEAARLRF
                     RPIIMTSMAFIFGMFPLVIATGAGAASRQSIGTGVLGGMLAATFLAIFFVPLFYVLIR
                     KLTGRMAVPSHQVHKASLLPEGEA"
     gene            complement(3499..4716)
                     /locus_tag="VFH05_03155"
     CDS             complement(3499..4716)
                     /locus_tag="VFH05_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6677237.1"
                     /translation="MLRPIDLTAAQTANRIHPLLTSAFLVFLATASLAGCKQDAGSAP
                     APQMPQVEVVTAATTTVPDEPEFIGQAEASRPVEIRSQVTGLIKAVLYQEGRDVKKGD
                     RLYQIDPVPFQAAAASAKAKIAQAEAKVVQAKQDLARVKPLLSEQAVSQKDVDDAVAE
                     DLAAKAALQGAKADLIKAQFDLDNTLIRAPIDGLIERTRYYEGRLVSAQTDLLTMVHR
                     VDPMYVVVSIPESFILKRRRDIEAKRIEHPGVYQLRGEVMLLDGSTYPHEGVLDLMEP
                     GLRTETGARVVRITIPNPKRELLPGQFVKVKFKGDVKPNVVLIPQRAVLQGPQGPFVY
                     VVGPDDHIELRPVQASVWQGDQWLIDQGIKAGDRVVVNGLMKIGPGAPVKAVPLGEAP
                     PPAASPSEPPKQG"
     gene            complement(4805..5086)
                     /locus_tag="VFH05_03160"
     CDS             complement(4805..5086)
                     /locus_tag="VFH05_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677238.1"
                     /translation="MLKITTHVSDDASRITLEGRLAGPWIGELERCWRESEQSAAGRQ
                     LIVDLTGVTFVEQQGKALLSRMCQSGAELLATGCCMRSIVEDAKNQIGK"
     gene            complement(5123..8224)
                     /locus_tag="VFH05_03165"
     CDS             complement(5123..8224)
                     /locus_tag="VFH05_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6677239.1"
                     /translation="MNMRSFTDVFIKHPVLAIVVNLVIVLAGWRALTTLPVQQYPKIE
                     SSSVVITTIYYGASAETVRGFLSTPVERVVSAIGGVDYVESTSRAGVSTVTVHLKLNH
                     SSTAALAEVTARLQQVRAELPAEAEPPVVEVQRADRPYASFYLSFSSGERTVPAVTDW
                     LLRTLQPQLSTLSGVQRVTIEGGRQIAMRIWIDPDRLAAFNLSPGDVQGALRRNNYLA
                     AVGRTKGNLVQVNLLANTDLRSTQEFEDLIVADRGGAIVRLKDVAQVEREAEEAEMVA
                     KYNRTEGVYLGVWPVPGANEIEVAGRLRDEMERIRPTLPADIDMRLVWDGTMFMRNAL
                     TEITKTLSETILIVALVVFLFMGSVRTALVPLVAMPVSLIGAALFMFAFGFSLNLLTL
                     LAIVLSVGLVVDDAIVVVENVERHVRQGKSRIEAALAGARELIGPIIAMTITLATVYT
                     PIGFQGGLTGSLFLEFAMTLAVAVVLSGVVAVTLSPVMSSRFVHPQGKEGRMSALVNR
                     TFELVRRQYARLLDRALDMRWGIVAGAVLVMIAAWPLYLFSRQELAPVEDQSHISLFF
                     EASPDSTVAATNRQHLQIVDAVTAFPETQFTWSLTTAWGGFGGLVAKDWHERARSTEQ
                     MYGQVFGAVSQVPGLRVFPRLDPPLPTPGQYDVELVLQSDVPAQQMLETVGAVLGAGW
                     QSGKFLYVDTDLKIDLPEARVVLDRERLADLGLDLAGVGRELGTLLGGAYVNRFNYFD
                     RSYKVIPQIGDKDRATVEPLLDLKIKTPAGQLVPVSTFTHIETSTGPRTLNRFQQRNA
                     VRIFGGVKPGVTKAEGLHILEAAAASSQGPRVVLDYAGESRQIRQEGGALTVTLGFAV
                     LLIYLVLAAQFQSFRDPLIVLLGSVPLAISGALVFSFLDLTTINIYSQVGLITLVGLI
                     AKNGILIVEFANQLQGSGLPRAAAVREAALTRLRPVLMTSAATVFGHLPLVLASGPGA
                     AARNSIGMVLVTGMTVGTLFTLFVVPVFYSLIAAEHQPDALGDAEVEMTEDDEALLAG
                     AKA"
     gene            complement(8250..9377)
                     /locus_tag="VFH05_03170"
     CDS             complement(8250..9377)
                     /locus_tag="VFH05_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6677240.1"
                     /translation="MKRRRHWVGSVVLPVIVLLMGVGLAAWKYDAIQAGQAASASQPE
                     PMETVTVALAKEIEHRQTTTSIGTVLALKSITLRNELAGTVREVRLSPGQIVEPGMVL
                     VALDVSVEEAELKAQEAQAALAKTVLDRRQNLNHEMATTQEEVDRARADLDIARAQIA
                     RTKAIIARKTIRAPFRSRVGIADVHPGQYLNEGTLLTTLQGIDDAAHVDFTVAQQVAA
                     GLREGEVVEVFAGTPAPITAKIVALDARIDPATRNAVVRARIEGSGNAPSPGSSVRVR
                     IPVGAPRKAVAVPVSALRKGPEGDQVFVIAQGPDGRARAHVQQVESGAMFGDEVVIHA
                     GLIAGEQVAASGSFKLRESALVAVAGAQTPQSEQRQTVSQH"
     gene            complement(9592..10578)
                     /locus_tag="VFH05_03175"
     CDS             complement(9592..10578)
                     /locus_tag="VFH05_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677241.1"
                     /translation="MELKMPTVEQAYWGLRAMKTVALADGALDDTERHMLASVQTILG
                     TNYVVEDLAPVTPEELASALIDPQIRHQLTQGLIVISLIDGKANVRETEAVEQFARAL
                     QVDAPEVRDLRYLINGEMLRLRLDLARRFWLREKVKDIWQHERLKGLYKFVRGMLGRY
                     EDAQLASRYQALAQYPPGSLGRSYWEYCRANGFPLPGEKGGAAEQVLFHDCAHVLSGY
                     GTTPDEEVQVACFSAGFQRRDPWTFVFFVLLQFHVGIRLTPITAARTGFFDPVKAMIA
                     IRRGAAMTVDLNNGWDYWPVMGEQVEELRRRYNILPAEAFLPGVRPLAASPA"
     gene            complement(10629..11582)
                     /locus_tag="VFH05_03180"
     CDS             complement(10629..11582)
                     /locus_tag="VFH05_03180"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6677242.1"
                     /translation="MNETLDFLTQHGSLVLAAAVFAEQIGLPLPALPFLIAAGALAGT
                     GQMAAGVALVSAVVAAMAGDQVWFELGRRRGRGVLNWLCRISLEPTSCVRRTEDFFSR
                     HGVRALIVAKFVPGFSTIAPPLAGIVGLSVPQYLLFNGLGTLLWAGTGIGLGWIFSDQ
                     VEQALTITAQIGPTIGLTMVAAAMSYVGYKAAHRYRMGDGVPRMTVRQLTDKLAAGEQ
                     PVIVDLRSLAARQEEPGIPGAVSLALEEMVTHQHGLPRDRDVILYCACPRDASSVEGV
                     LRLRKLGFTRVWPLAGGLGAWNAAANPPSAVQLSDRQIVPA"
     gene            complement(11595..12059)
                     /locus_tag="VFH05_03185"
     CDS             complement(11595..12059)
                     /locus_tag="VFH05_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677243.1"
                     /translation="MNRTSPTIDLFTLIGMMATIAGIFLCLFFLFTPTTFGAPAIDVL
                     KDRSADLYISMRWLQPTLGQAIVEDALIRQRDSSASGAVKLQPGVGSVQWVIGRVVVE
                     LHRSRLVSALPGGRQEDDQRIVRIARHAAVQLQEHLTGEERPRVTGQADVIF"
     gene            complement(12271..14850)
                     /locus_tag="VFH05_03190"
     CDS             complement(12271..14850)
                     /locus_tag="VFH05_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6677244.1"
                     /translation="MDHPASPCETLGERYQALLEVAEAIAAHRDVHELFRDLARRLPR
                     VVQVNFVALSLHDPTGNRMRLQTIQANVPADLVGGHEEPLEETPAGTVWQSQQPILVP
                     DLGEELRWPKVIERMQQDGVNSFCVVPLTTAVRQLGAMGFASLRKHAYGEGDIEFLRE
                     VGKQVAVAVDNVLHHQDLLHDRDRLRLLLEVTESIASHRDLAELLRDLAQRLPSIVPF
                     DYINIVLHEPARQVMRLWLLVASVPSTISPGLETPVEESPGGWVWTHQEPLTVNDVSQ
                     EHRFPKLMSILRENGVQSFCAVPLTTAQRRLGAMGFGSLQQRTYRDADLKFMQQVAKQ
                     VAVAVDNAFNSETTRAYQQQLARERDLQRLLLEVNNAVISHLELRDLLKAVSVSLSRV
                     VPHDLAGFALFDQDTQRLLAHALDFPSNQSFVETGHAIPLEGTPEGRAFTSRQTVLLR
                     NIDTAEFPADIMKRAKAEGLRSGCAVPLVSHGRVLGTLSVVSMREEAFSEQDAELLTQ
                     IGAQVAIAVENALAYKQIAQLKDKLTEEKLYLEEEIQTEYNFQEIVGESQTLRRVLKD
                     VETVAKTSSTVLILGETGSGKELIARALHNLSDRRERTFVKLNCAAIPTGLLESELFG
                     HERGAFTGAIATKIGRFETADRGTLFLDEVGEIPLELQVKLLRVLQEQEFERLGSTRT
                     QRVDVRLVAATNRDLGHMVEEHTFRSDLYYRLKVFPVTVPPLRERAEDIPALVRHFAQ
                     KFALRMNKRIETIPAEAMKALQAYPWPGNVRELENFIERAVILSAGTDLFVPLSELKV
                     SIPPSNGSPKTLEDAEREHILKALRDAKWTIGGSAGAAAKLGMKRTTLQSKMQKLGIV
                     RPG"
     gene            complement(14893..15339)
                     /locus_tag="VFH05_03195"
     CDS             complement(14893..15339)
                     /locus_tag="VFH05_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6677245.1"
                     /translation="MKARYLGHVVFYVKDLEQSLAFYRDLVGFKEVGRIFGGAAAALT
                     SGRTHHELLLLQVGDAPPPPSGRRRGLYHIGIKVGDSLDELRSAKRELEQAGITIDGM
                     SDHTVSQSLYLRDPDGNEVELYVDADEAVWKADPAAVLSPIKSLRL"
     gene            complement(15336..15752)
                     /locus_tag="VFH05_03200"
     CDS             complement(15336..15752)
                     /locus_tag="VFH05_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677246.1"
                     /translation="MTQRGHSSEVLQLSKALLHLHKSLLDGERAAYERLHGPICSNGA
                     FLQLVLGHPSFAWLRDLSRSMARLDELAEGKDASCAEALHAQTASLRTLLVPRKDGEE
                     FNRRYHDALERDPDAALAHAAVVELLAEPRRQGGES"
     gene            complement(15755..16189)
                     /locus_tag="VFH05_03205"
     CDS             complement(15755..16189)
                     /locus_tag="VFH05_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6677247.1"
                     /translation="MPVQVYGCNHVVIEVTDAKKAVQFYSDVFGLKMLRGGEGAAWCK
                     LGEHQFMAIFEVERLQPDRVKHFGLMVRDPKQINEVRRKLTNKYKLKLHPGFRCDFRD
                     PWGNRIQVGDLSDESLVWLLPYQEVQKVGLSFTSASKRRRRD"
     gene            complement(16193..16858)
                     /locus_tag="VFH05_03210"
     CDS             complement(16193..16858)
                     /locus_tag="VFH05_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET6677248.1"
                     /translation="MDDQNASITVEVYSDVVCPWCYVGKRRWERAQSQFPTAATQVIW
                     RPFQLNPTMPEEGMDRRIYLEAKFGSQDSYQRLEEQVAAAGASEGISFAFERMRKTPN
                     TFLAHRLIWYAGQHDRQNCVVEALFKAYFERGADIGSQAVLADVAASAGLKAARFLQT
                     EEGAANVKAEEVAGRRLGIRAVPYFLLNKSYGISGAQPVDVFLGAIKSIRVRQAEDHL
                     TRK"
     gene            complement(16965..17861)
                     /locus_tag="VFH05_03215"
     CDS             complement(16965..17861)
                     /locus_tag="VFH05_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET6677249.1"
                     /translation="MTMDVMTKKEVLGVYQPGSAHMVGDGFPVRNLFPSNELDQEVDP
                     FLMLDYAGPRHFPPTEHPRGVGEHPHRGFETVTIMYEGAVAHRDSAGNAGVIGPGDVQ
                     WMTAASGVVHEEMHEAQWAKQGGTFRAIQLWVNLPQAHKMSAPAYQTLVNDAIPAIDL
                     EGGSGRLRVIAGTFRGRKGPARTCTPVELYDLQLQAGARFELTLPEGHNTSVFVLEGR
                     VSVNSAVAGEAELVVSKGQGSLVSLEANDHSRLLIMSGKPIKEPIARYGPFVMNTKAE
                     LVQAVEDYQRGRMGHLSSPDAE"
     gene            complement(18181..19308)
                     /locus_tag="VFH05_03220"
     CDS             complement(18181..19308)
                     /locus_tag="VFH05_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6677250.1"
                     /translation="MFSVPRLWAIVVKEFIQMRRDRVTFGMMIGVPLIQLVLFGYAIN
                     ADPKRLPTAVLMADYGPQGRTVLHAIRNSGYFEFTRELTSEQEGRDVLARGDVQFVVN
                     IPQGFTRELLRGERPAILIEADATDPAATSNAIGSLRMVLTKALEHDLKGPLVFLAGR
                     EDPIELRVHAQYNPEAITQYNIVPGLMGVVITMTMVMITGLAITRERERGTMENLLSM
                     PTRPFEVMVGKIIPYILVGYVQLGLILLAARVLFDVPMHGNLGLLLLVAFLFITANLA
                     MGITFSTLAENQLQAVQLSFFFFLPSLLLSGFMFPFRGMPQWAQAVGELLPLTHFLRV
                     VRGILLKGNGLHEIALQLWQIALFAVVALAVGIKRYRQTLD"
     gene            complement(19320..20249)
                     /locus_tag="VFH05_03225"
     CDS             complement(19320..20249)
                     /locus_tag="VFH05_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6677251.1"
                     /translation="MTGQALAIDVHGMTKRFGERTVVDHIGLQVRRGEIFGFLGPNGS
                     GKTTFIRMLCGLLRADEGSGTCLGYDVVRDSESIKAEVGYMTQRFSFYEDLSIAENLD
                     FVARMFAVKNRREAVARSLERLGLAGRQRQLAGQLSGGWKQRLALAACLIHQPQLLLL
                     DEPTAGVDPKARREFWEQIHELAAEGLTFLITTHYMDEAERCHRLAYIAYGRLLTHGT
                     VTEVIAQARLTTWSVSGPNLHHLAEELRKHSGVRQAVAFGNRLHVSSDDEKALEAAIL
                     SFRKPPYEWRPISSGLEEVFIHLMNRTPDNFTS"
     gene            complement(20246..21253)
                     /locus_tag="VFH05_03230"
     CDS             complement(20246..21253)
                     /locus_tag="VFH05_03230"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6677252.1"
                     /translation="MRIGRVGTTWRQAFILPIVMMTAGFLFACERTPSDRVQGYVEGE
                     FVYVASPFAGALESLSIQRGRQVRSGDALFALERGAEKAARDEAERRLGQALANVEDA
                     KKGKRPSEIDSLKAQLKHAQAALRLSTREVVRQEGLSQVPGAAVELEVDRARAARDQD
                     RQRVAQLQADLDTALLGSRTDQVIAAEAEVHAREAALARADWEFAQKRQQAPKSGLVF
                     DTLYREGEWVPAGRPVVVLLPPENIKVRAFVSETRVGTIRPGDHVQVAVDSVPQPFMG
                     TVSYISPRAEYTPPVIYSQESRDKLVFMIEVVFDPQSAVNLNPGQPVDVRFESSSGGG
                     R"
     gene            complement(21314..21787)
                     /gene="smpB"
                     /locus_tag="VFH05_03235"
     CDS             complement(21314..21787)
                     /gene="smpB"
                     /locus_tag="VFH05_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET6677253.1"
                     /translation="MTKENAAEGKVVATNRKAYHDYFIEEKYEAGIMLQGTEVKSLRE
                     GRVNLQDSYASVRGTEVYLHQCHISPYSHGNIMNHDPTRVRRLLLHKVEINKLLGKTQ
                     QKGLTLIPLRIYFSKRGYAKVELGLAKGKKLYDRRESIKAREAGREVQRAIKERK"
     gene            21843..22310
                     /locus_tag="VFH05_03240"
     CDS             21843..22310
                     /locus_tag="VFH05_03240"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HET6677254.1"
                     /translation="MRPSGSLDSFSIILSGLAKRLGLQTHLFEVRLQQQWREIIGEPV
                     GSHTWPVQVRFKKLYLIVRNSVWLQQLMFLKPTLLAKVNEQAGSELITDIAFRVGEIP
                     DARTAAPASQRQQQPVNEAAVVEANAHAAAVQDPELRNRLASLMADAFSRPRS"
     gene            complement(22307..22879)
                     /locus_tag="VFH05_03245"
     CDS             complement(22307..22879)
                     /locus_tag="VFH05_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677255.1"
                     /translation="MNNATSQSPPLSPPKKSLDTVVKLALMVFFGSFALIWGGMYLSR
                     PDRSIPPYSIGSQEDTAVSVHVPAWTSDMEIETLIERFRKVGRETRNFGPMKIRPTTP
                     DDPSGHYRRITIYIFTHDAWAEAPILHKYLTGEDQEVRDGFKRALRGLYRLTETDEEG
                     RIGPIVDGPDSAATAAYSRQLFKRSLLEQP"
     gene            complement(22906..25386)
                     /gene="gyrA"
                     /locus_tag="VFH05_03250"
     CDS             complement(22906..25386)
                     /gene="gyrA"
                     /locus_tag="VFH05_03250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET6677256.1"
                     /translation="MPPDERLGQIAIEDEMRSSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRILYGMNEMGLGHNRSYRKSAKIVGEIMGNYHPHGDSAIYDTLVRMAQDFNMRYPLV
                     DGQGNYGSMDGDSPAAMRYTEARMTKLAEEMLADIDKETVDFGPNYDESRQEPLVLPT
                     KVPNLLINGAGGIAVGYATNIPTHNIGEVIDGLLLLLESPDVTVAQLMKKIPGPDFPT
                     AGFIYGMSGIKEAYETGRGLLALRAKVVVETDERTERERLIVTEIPYQVNKAKLIEKI
                     AELIQEDRIKGISDLRDESSDREGVRVVIELKRNEIPLVVLNNLYKHTQLETTFGVIM
                     LALVNNRPEVLNLKQILHHFLEHRREVVVRRTTYELRKAEERAHILEGLKIALDNLDA
                     VIALIRRSQSPDEARTGLMRQFTLSEIQANAILDMRLQRLTQLERAKLVEEYQEVLKQ
                     IEYLKSVLASEGLVRSIIKDELTQLREAYRDDRRTQIVKEEAEISLEDLIAEEEVVVT
                     ISHAGYIKRNAASLYRAQRRGGKGKIGMGIKDEDFVETLLTASTHDTLLFFTDAGKVF
                     WLKVHEIPEASRAAKGKALVNLLALAGSEKVTATLAVKDYPADRFVVMATKKGVIKKT
                     ELSAYSNPRQGGIIALGLEDGDRLIGVQVTDGQREILLGTKQGITIRFSESDVRPMGR
                     TAYGVKGITLEEGNEVIGMETITPDSTTAILTVTEGGYGKRTPVGEYRVQGRGGKGII
                     SVKTTERNGLAVGFLQVRDGDEIMLMAAQGKVLRCKVGDIREIGRNTQGVRILDLDGD
                     DDRVVAVARLADAVEREEAGPEEGPAGQ"
     gene            complement(25547..28015)
                     /gene="gyrB"
                     /locus_tag="VFH05_03255"
     CDS             complement(25547..28015)
                     /gene="gyrB"
                     /locus_tag="VFH05_03255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET6677257.1"
                     /translation="MATDETTKPKSDSYSADQIKVLEGLDAVRKRPAMYIGSTGVDGL
                     HHLVYEVVDNSVDEHMAGFGETIEVTIHIDGSVTVVDNGRGIPTGMHPTQKKSAAEVA
                     LTVLHAGGKFEQGAYTVSGGLHGVGISVVNALSEWLELEIWQDGQVFEQRYERGKPGA
                     PLKDTGITKRRGTKVRFRPDGQVFETLEFSFDVLAQRLRELAFLNKGLSITLKDERKE
                     PAKEQVFLYKGGIVSFVEHLNEAKTPLHKPIYVRVEKPEMILEVALQYNDSYAENLFS
                     FANNINTREGGTHLVGFKAALTRTINTYANANELLKKETESLTGDDVREGLTAVVSVK
                     VRNPQFEGQTKAKLGNSEVKGVVETAVNEKLGAYFEENPTVARKIIGKAIDAARAREA
                     ARKAKDLIRRKSALDGGSLPGKLADCSEKDPALSELYIVEGDSAGGSAKQGRDRKFQA
                     ILPLKGKILNVEKARFDKMLTSDEIRTLIMALGTGIGRKREESDKADKDTFDIAKARY
                     HKIILMTDADVDGSHIRTLLLTFFFRQMPELLERGYVYIAQPPLFKVKKGKVERYLKD
                     EGALNEHLADLAVEDVHLYLEGAQDFVTGRRLLPILKKMISFETLLARFNKKPHEASM
                     IRAFVDEPGLDRDLLKDGAALTNAVANVKKTLRVMYPKLDSTLEILSDEEHHSNKVSC
                     RMQANGSSFGLDVTHDLVGSADFRELQKLAPSAIGLGRAPYKLKAKGEEQELRSTSDL
                     VRTILDLGKQGLSIQRYKGLGEMNPGQLWETTMNPEARTLLRVTLEDVTGVDEIFTIL
                     MGDEVEPRRNFIQTHALEVRNLDV"
     gene            complement(28283..29410)
                     /gene="dnaN"
                     /locus_tag="VFH05_03260"
     CDS             complement(28283..29410)
                     /gene="dnaN"
                     /locus_tag="VFH05_03260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET6677258.1"
                     /translation="MKVRIGRDELLTGLQRVQGVVEKRNTMPILSNILIEAKQEGAEI
                     VATDLEIGMRGLYKATVHKAGGITVSARKLYEIIKELPSGDIDLISSENNWTTIQAGK
                     SQFKVVGLPSTDYPALPAIDREGLTPLAGAGLLELIRKTLFAAGDNDARYILNGLLVT
                     LISGEKKTTLRLVGTDGHRLAVAEQDLGKAGAKGAPQEIKAIIPKKAAQEMRRLLEEG
                     GDGEPLIGFTKNLMIFRKSGLLLTSRLMEGNYPNYQQVIPKESNKKIMVVRAELESAL
                     RRVSVLSKDKANAVKLSFAPGRLTLFSSNPDYGEATEEMSAQYEGEALNTGFNARYLL
                     DVFDVMDGEKISLQMDSALSPCLIQEAETPGFKCVVMPIKI"
     gene            complement(29432..30775)
                     /gene="dnaA"
                     /locus_tag="VFH05_03265"
     CDS             complement(29432..30775)
                     /gene="dnaA"
                     /locus_tag="VFH05_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET6677259.1"
                     /translation="MTVSTVWQEALAYIQGRVPKQVYDTWFMPVRLERIEDRSAQIGV
                     PNKFFGDWLSQHYGPLLEEAVSTARGGGETAVSFVVFSRPPISQSDVAAVAQAVRTAT
                     TPRSKRGIQLNPKYSFKNFVVGAGNQFAHAACMAVAEQPAKAYNPLFIYGGVGLGKTH
                     LLNAIANHVAERTDLRIAYLTTEQFTNEVINSIRYDKMMDLRKRYRHIDMLMIDDIQF
                     LAGKERTQEEFFHTFNALYEAHKQIVLSSDRFPKDMPDIEERLRSRFEWGLIADLQPP
                     DVETRIAILRKKSEDEGVTLPEDVIQFLSTTMKSNIRELEGSLVRLGAYASLTGQSIT
                     LEMARNVLRDLIGDKKKIVAMEDIQEAVAACFHIKIADLKSRRRSKTLVHPRQIAMYL
                     CRELTDASFPEIGRLFGGKDHTTIMHACRQIMKAKENDSGLNQTLDGLKEKILRG"
     gene            complement(31107..31337)
                     /locus_tag="VFH05_03270"
     CDS             complement(31107..31337)
                     /locus_tag="VFH05_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677260.1"
                     /translation="MKNTLYLLRKPLDRVDPALFIASHSIGDVVLLDRALVPFPHSGG
                     TVFSLGTEDGDRTISYDVLVKKIFASDAAIVI"
     gene            complement(31334..31678)
                     /locus_tag="VFH05_03275"
     CDS             complement(31334..31678)
                     /locus_tag="VFH05_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677261.1"
                     /translation="MSKRVAVVIAEDPTETHRPVEALRIALGLCAGDHETTIILLGKA
                     PLLLTDDTDDVIDVDILEKYRPSFQQLQVPFVVASGLPMQKFLDGFSVTSKPTDEIRT
                     LVHSLDRTLVFS"
     gene            complement(31692..32012)
                     /locus_tag="VFH05_03280"
     CDS             complement(31692..32012)
                     /locus_tag="VFH05_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET6677262.1"
                     /translation="MAQQKLGLLLSTAPSHPSVETVVQLAEAALRQGVEVYLYLIDEG
                     VRSYMDTRFRGLLDAGVKMSVCAYGCQQHGVSTDQVDPRVSLSGLVVLSGIIDGCDRF
                     LAFT"
     gene            complement(32125..33381)
                     /gene="hisS"
                     /locus_tag="VFH05_03285"
     CDS             complement(32125..33381)
                     /gene="hisS"
                     /locus_tag="VFH05_03285"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET6677263.1"
                     /translation="MIKGIKGVKDILPEETPRWRFIEETARQWADRYGFHEIRIPIFE
                     VTTLFARSIGATTDIVEKEMYTFPDRDGTSLTLRPEGTAGTVRAFIEHHRGADPLPQK
                     YFYIGPMFRHERPQAGRLRQFHQFGVEYLGTQDPKADVDVIALLWRFLSDLRLPDLTL
                     EINSLGTSTDRAAYLPVLVAFLQTRVGQLCENCRRRIDTNPLRVLDCKVPACRAATED
                     APHLTSYLSPEAKDHFDQVLSTLSALGISFRLNPRLVRGLDYYCLTAFEITSSHLGAQ
                     NAVGAGGRYDGLVELLGGQPTPAVGFAAGLERIAMMLPSERVPTLRPSVYVASFGAEG
                     SPAGVRLLDALRQVGLSALSDYRATTLKAHLRQADRAGCRFAVLLGDDEARAGSAVVR
                     DMESKVQETIPLTDLPRYFVALSKTF"
     gene            33537..35024
                     /locus_tag="VFH05_03290"
     CDS             33537..35024
                     /locus_tag="VFH05_03290"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET6677264.1"
                     /translation="MSSDRPTRRIIAVAPMPPEKSAYALARYSRSPDSIEQSIKWVHG
                     HSSEKFWEQFYFDYGHASIADLGHVIICFEEITELAAIRLEDEPVWDGQAKSSRYQNF
                     ASGGWYVPDAIHGSETEGTYHGILRSLGEIYRLLHEPLVTYLSEREPRPPSMKPDTYQ
                     RTIAARAFDVTRYLLPLACKTNVGQVVSIRTLEKQITRLLSSQLPELRMIGEDLKDAC
                     QRPPMNVWGELCGQTAGLNEPMAPTLSRYARANDYQATVYTDLARYAKEALRGTGLDH
                     ADTWGTCDPVELIDPHDPIDETVATLLYRVSHAPYRRILGVVRDWTEKQKQDTIAVAL
                     SRRGSYDELPKEFRSGYAFTFDVLMDIGGWRDMHRHRRCQQIQQNFTTAHGYDVPAPL
                     IEARLDGEYRQAMDAVRADIEQLKKVNGEAALYAIPFGFKVRCLFKMDFAEAEYIAKL
                     RSGVKGHWSYRNIAWLMKEKLTQRYPMLGVHVQATSPDIEDTLTR"
     gene            35039..36079
                     /locus_tag="VFH05_03295"
     CDS             35039..36079
                     /locus_tag="VFH05_03295"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677265.1"
                     /translation="MTEHQISCETALLDGDWEQAAHEALAWARHPKSTDRRDPRPHFA
                     LNVVHLIKGQFAEAWSSHALSLQEPDDIAQVKSWLDGLVARHADQPNAHLAMGLFLSQ
                     SGQSEQSMQSYRTAAKLAPQSAYPHYFLAQIHERANHLEMAIKEYREAVRLNPDYVPA
                     RTNLGVAYQEQGRLEMAIPQYREAIKLNPSDAVAHANLACALAEQGKMQAALLEYQEA
                     LRLNPRDAEVHFALGGLYENKNRPDLAMKEYRAAIESNRDLAPAHSALGWMLFDRGHR
                     EEAMECFNQALRADPQNARAYLGVAKCYASRGKVQLAIENYTKAIKYEQDPQKKNEIM
                     NNLYKEGGTWDT"
     gene            complement(36119..36388)
                     /locus_tag="VFH05_03300"
     CDS             complement(36119..36388)
                     /locus_tag="VFH05_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677266.1"
                     /translation="MKGLRFERIGQGRYYDVVFHLGSTYVPVSDETIEELKAQSLLPA
                     ERFLDLLIDRVGYSSYLKDQIRKELKSSGDPVTQITVLQGAIREL"
     gene            complement(36484..36819)
                     /locus_tag="VFH05_03305"
     CDS             complement(36484..36819)
                     /locus_tag="VFH05_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET6677267.1"
                     /translation="MGLILHPMKEIRVIVAGEQRAFVTDLLDRVNATGYTIIGNVSGK
                     GHHGVREAHFMFSEQESLEMIMTVVPQEKVEPILAGLRPLFERHSGVMFVADVAVSRQ
                     EYFAKSKPA"
     gene            complement(36882..40082)
                     /locus_tag="VFH05_03310"
     CDS             complement(36882..40082)
                     /locus_tag="VFH05_03310"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HET6677268.1"
                     /translation="MEQVDRTIDIQTRRMELRGIVKLAGEVIAQYWPMRTFVHHNPLH
                     SLEYLPFEETVRRGKQFMGGNGYLPGRMYRQYLRSGRIQLRHLDEALSSRVQGKEVVV
                     GSRIVTHGEVLRGCLTEGLCAAVVEPLDNQLPDDSLGVVERLAGRLESVLTVPDLAER
                     IRGIVDSDEAALGRWLTLSHWCDDTLGTHIVGQINDQMIKWCEAFLDEGHATWAMPER
                     EKGFYKAWKDIASHEWSPCGITDSKRKIAQLPEYPEDALLEHLDALGIPARLRQDYLS
                     LQLTALPGWAGFIKWREEERDYPWQQAYPVGLVKYLAIRLWYARELVHKACSSHLGIE
                     GRYDAVVAYMRSHAEEYYLRRQRVAGRLPALYAEEVDRLAHQKGAGWKTVLDRYKAEV
                     VPRQEAATRRGAARKLLALGRCLEIDPEQLVETDPETLQQLVEWLEAFPESEHGPVWL
                     NAFEAGYQERLLGALRARTREAEHPNATRPYSQSVYCIDVRSEPFRRHLESTGPNETY
                     GFAGFFAAFIRYRAWGKEHDTEQFPVIMRAKNEVREIPRSYLDHRVSKHKARAKWVRA
                     GHTLLHDLKENVVTPYVMVESLGWFYTLPIFGKTLVPSLYRRWTGWLRRLFVPSIATT
                     LTVDKLAPQDTAEMLVADQQALVRKALQERLGLRASRITPGLVEGLRQRALTDSHEHD
                     EAVIEAANRAGLSADALQGFVEVLRREYDLNARSASRQKERITRTGFTLEEQALTVDT
                     ALRMMGLTKTFARLVLFCSHGSTSDNNPYESALDCGACGGNEGKPNARVLAMMANNPK
                     VRERLSKGGIDIPNDTHFLAGQMDTTTDEVQLFDLEDVPPTHRADLARLQEDLREASA
                     LTSLERCARFPDVPQSLEERQAQTHVRRRSADWSQVRPEWGLSGNTAFLIGRRELTKG
                     LNLDGRVFLHSYDYREDPTNRLLEVLLTAPQVVGQWINMEHYFSAVDNDVYGSGSKIY
                     HNVVGRLGIMSGPWSDLRLGLARQTVMNGSMPYHEPMRLLTLIEAPRERIEKLISRHE
                     VLQHYYHNEWVHLVALDPEDKIWYRYHPKGVWHVIP"
     gene            complement(40070..41380)
                     /locus_tag="VFH05_03315"
     CDS             complement(40070..41380)
                     /locus_tag="VFH05_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6677269.1"
                     /translation="MIDGALLSWLLTGIPLLGAVGSLLFWPNADRVKFFSILWSAVSC
                     ASIIGFTGYLAMPPEGFLPLYLLPMAAIISLLAQPAHDAYRQSCIMTLVCLGLGLTVL
                     TSVPLVGNLALALLLVLVAILLYRHHSALWPMSWWGIGTYGLGALCALLAVVAGPPVS
                     SIASLVTCAVLLPLVPFHDGHLTALTRLPGNLPAFIVVLLPIAGLHELPHVLPTVPDV
                     VAWTVSFFALLGALYGAVKALAQSRVRLLLAYGSLSFFSMLWWFVAATRTATPRAALM
                     AGTVGLATSGLLIAWQVVRTRYGDDIDPQAVSGLASGMPQYAVLLSLLALAAMGLPPF
                     GVFAGFMGLLLNAPFPSTVGLFITLLAWLAASWYIMQMVQRLLYGTRRQELRYTDVLK
                     TELASLVMAVLVLLALGLAPATLFAPGHTGSETVSVKESSTWNR"
     gene            complement(41377..43077)
                     /locus_tag="VFH05_03320"
     CDS             complement(41377..43077)
                     /locus_tag="VFH05_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6677270.1"
                     /translation="MTLTLLVPLLLLVATGLVLIGSDSSRHARAKMAAYPIGVAFLAA
                     FATLLQVTTGGPIALQFYDLSSVGTFAIPIGFYIDRLSAVMMTLITGVSVIIYTYSIG
                     YMYQDAHARRYLAMICLTDFVLICMVASSNLMMLFLFWQVLSYLLYVLAHNHAHAGTL
                     TGAFKTFTVLRVADTAFLAGIALAYQLYGTLEFQDLFLRAAETPIGLPLGFGMEVSGA
                     TAVTFLFFVGAMGKSAQFPIHFWLPGSLFAPTPVHALLHAGIINAGGFLINRLAPLFA
                     LSSTTLHVAFVIGTLTAVLGATMMLAQNDIKKTLGFSTIGQMGYMIMECGLGAFSLAV
                     FHLIAHGLFKATVFLNCGNVIHKARQEPHFPHVDHQREEEGLSRLTWVTGFVTTLLIP
                     LFILLLTHGVLRMPLLESQGTVIFLFFIWITSSQAILTLTRLRQVASWKVSAAMLVTL
                     LFIVFVYLFAVESFTAFLYPNPDEVASYFKAAALPNWLFDFIILAATILMIASWSYLY
                     MTAHGRTIRMPGWIDNLRIRLYLPFMNRLYADELHQLLGDSLMRWVHQFDKREGGWSR
                     "
     gene            complement(43206..44903)
                     /locus_tag="VFH05_03325"
     CDS             complement(43206..44903)
                     /locus_tag="VFH05_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET6677271.1"
                     /translation="MTAALLVPLCPLLTVLLVIAGNESTRRLRVKIGAWPLAVALLGA
                     LCTLYVVATSGPISIRFYEPAEGSALPVPIGFHIDRLSAVMMVLISAIGTIIYTYSID
                     YMYQEPHERRYFAMLGVAICVLLCMVSSGNLMMLFVFWQILGYLLYVLIHNHAHAGTL
                     DGAFRTFTILRVGDVAFLAAIALAYSLYGTLEFPDIFAAAAQSTATVSLLPGIELNGA
                     TAVSLLLFIGGMSKSAQFPFHIWLPRYLYAPTPVTALLHAGIINAGGFLINRLAPLFG
                     LSSTTLHIAFLIGTLTAVLGASMMLAQNDIKNMLGFSTIGQMGYMVMECGLGAFSLAV
                     FHLIAHGLFKATVFLNSGNVIGRARQEPHFPHANHDTDEHGYSPLTWFTGFFTTLVIP
                     LLILLLTHGVLHIPLLEAQGTVIFLFFIWVTSSQAILTLTRLHAVASWKVSAAMLLTL
                     LFVVFVYLFAVESFTAFLYPDPQEVALYFKAAELPGRLFDMMIGAATLVTVIGWCYVY
                     MKAHGRSLPLPTWIESIRIKLYVLFLNRLYIDEGAERLEQARRSLLQRLEKHAEERV"
     gene            45191..45607
                     /locus_tag="VFH05_03330"
     CDS             45191..45607
                     /locus_tag="VFH05_03330"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HET6677272.1"
                     /translation="MVRQRAQDLLVHDSVQLYNSAGSTVETAIRNAIIKFEQEFMGRG
                     PDDVRASIVRDLVVVRLKGVLTPAERQLAKTAEGVDMVKRLRQNLIAQGRDKLCEQVS
                     EITGAKVLGVFTDIDVQLGERVFVFTMDRELQNGGR"
     gene            45657..46304
                     /locus_tag="VFH05_03335"
     CDS             45657..46304
                     /locus_tag="VFH05_03335"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET6677273.1"
                     /translation="MEKLVKGFLKFHGEVFGRKKDLFIRLSQKQEPRALFITCSDSRV
                     DPTLMTQAEPGELFILRNAGNMVPPYGSMQGGSTATIEYAMAVLKVPHIIVCGHTDCA
                     VMKALLHPEEVQELPAVREWVGQAETTRRLMREHYTDLEGNARLIKTTQENVRAQLDH
                     LRTHPSVALQLRRGKVDLHGWVYSILTGDLWVYDFESEQFTPLVDEAPPRLKAAR"
     gene            46400..47875
                     /locus_tag="VFH05_03340"
     CDS             46400..47875
                     /locus_tag="VFH05_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HET6677274.1"
                     /translation="MGDRQLETLTFHNTYARLPEAFYAKLNPTPFSSSPYLVSFNAAA
                     ADLIDLDRREAERPEFTALFGGSMLTPAMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     ATNARGERWDLHLKGAGLTPFSRDGDGRAVLRSTIREYLCSEAMHALGIPTTRALSII
                     GSDEKVYREQVESGAMLLRMAPSHVRFGTFEIFYYRRQHEHLRVLADYVIDYHYPHLR
                     ETPDKYAALFAQVVERTARLIARWQAVGWAHGVMNTDNMSILGLTLDYGPFGFMDDYD
                     AGFICNHSDHNGRYAFNQQPFIGLWNLSCLAQALLPLAEKDALKEGLDGYMPLFEREY
                     RSLMRAKLGLADARDEDEELIREFLGLLQGSHADYTIVWRELGGFLSGRGMVNDRLRE
                     HFLNRERFDAWAVQYAERLRAERSRDDDRQQAMNRVNPKYVLRNYLAQAAIEKAQQKD
                     FAEIDRLLALLQHPYTDQPGMEGYAAPPPNWGKHLSVSCSS"
     gene            47999..>51062
                     /locus_tag="VFH05_03345"
     CDS             47999..>51062
                     /locus_tag="VFH05_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677275.1"
                     /translation="MAKPRGDSKLAVAGALTLVLAIAGVLLVKEPLRSSRPVGSGLEM
                     KQSTAEQLVRARLWEDPVAAVDRAIREQGATKTTSRGSSLAKRLSPLRHAIAQRVHRG
                     EQLTVLLITTTGGGYVESTESRLRDRYAVGTALGAACYAPEDEGHLSFVHWEPGSTVQ
                     GLPYEWYRLRKTRVCGAAGSRADSILIVWLPDEAVTAGFFATLTSLSQGLVCREDSNN
                     ECLLNGAKPSLVRLDPTLQQAASFKIVGPRSSSAYRALLDEAGALTGNAQQGVGIWPN
                     ADGWIELYSPWASAMKGLLAYGLKSESGSSCTSYEECEHRFYRRLSDAKVRVLYDVGS
                     DEIRFQSLIAELERRQVRLGWDAVILIGEWDSFYGRTLPIEFRAAACAKIATFPETDL
                     KAIQVPVQVKQWCQTIAQAIDLQIQRPADYESLLLNVFRYSYLSGLDGEIPGDDAARA
                     ARAAKAGADAKGKDATRDRPEGTGQTDYVRALVARIHDEGEGARAIGILGTDPYDALL
                     IIKALRPAFPHAIFFTVDLDARHLHPSEYKSTRNMVIAAPFGLQLEGGLQRDVPPFRS
                     SYQTSAYFAVLQAVGHVRCEQQGHQQQTGPCTTSYHVAQTPDDRLYDAASHPRLFEVG
                     REGAVDLSMVEKEGVRTIHPLRADLDYTDEHGQLKQGIGFDDSAVAALLAVGVVLGTI
                     IVWSNQRLWLWVSRNPVWIGLSAVVLLAAFWAFVFTGGGSMLLAGHDEGEPFSWTAGV
                     SIWPSELIRLFVVVLSLALLIKGSRDLMRNSDLISRDFGLARHTAHGTFSLKTFWMNL
                     KRVSHPTETMTPTTLDQAWQWYSEASRPAQWLARTVFLFLLYLGIMWSLSAYVVDEEY
                     IHPCRGRLSCTIDSFMTLSSAALVVFLNLAVFDAVMLCRRWIGWVTASTGGWSEQVQE
                     KYLREYGLRPDQKVEFEKLKYLAAVDLIGRRTEVVNRLIRYPFIALLIMMAARNDYFD
                     IWNYPLLLLFSWAVNVVLALMAAFLLYQAASQAKAAMLAGLSRQMVQAL"
BASE COUNT        10730 a        15226 c        14641 g        10465 t
ORIGIN      
        1 ttacggccga acgatggagc attgccggac gccgtcatat tgggaagaaa atcagatttg
       61 gccacccagg ccctggcctg aaactcctcg acggtcgccg cggctttttg cagatttcta
      121 ttttcagcca gcgcagtgcg gaccaagtgt tgaagctgct cgtcctttaa gagttcccac
      181 cacggcaggt tcgccattga aggcgcgtcg gcgcccgctt cggccatccg gaaacgatct
      241 tcgctatcgg cacccggccg ctcatagtct ggtcctaacg cacaagccgc cgtaaggact
      301 gcgatcgtca tcaagagata gggacgcata tcaggcttct ccttccggaa gcagcgaggc
      361 cttgtgcacc tggtgtgagg ggaccgccat ccggcctgtc agcttcctga tcaacacata
      421 aaacaacgga acgaaaaaaa tcgcgagaaa ggtggccgcc agcattcctc ccaacacgcc
      481 tgtgccaatc gactggcggc tcgcggcgcc cgcgcctgtc gcaatgacca atgggaacat
      541 gccgaagatg aaggccatcg acgtcatgat gattggacgg aaccgcagcc tggcggcttc
      601 gatggccgct tcgatcagtg gcttaccggc ttcatagcgg gtgttcgcaa actcgacgat
      661 cagaatggcg ttcttcgcag acagtccgat gagcgtcacc atgccgatct ggaaatagat
      721 gtcgttcgtg aagccgcgca cccacacggc cgccaatgca ccgaaaatcg caaacggtac
      781 ggccagaatc accgcgaagg gaacggccca actctcgaac tgtgccgcga ggacgaggaa
      841 gaccatgaga agcccgatca ggaacacctg actggactgg ccgccgaccc gacgctcctg
      901 gaatgagatg ttgctccaat cgatcgcata gcctttcggg tcgaggacct ccttcgccac
      961 ccgctccaag gcctcgagcg cttccccgga actgtagccc ggtttagcgg cgcccaggac
     1021 aagcgccgtg ttgtatccat tgaagtgatt gaccggatca ggcccgcttt gatactcgga
     1081 cgtcacaacc gtatccagcg gaatcatgtt cgcaccttga gcggtctgcg cgcgaacgta
     1141 aattttcgaa atgtcctgtg gcgttgaccg gaagtgagga tccgcctcgg tctggacgtg
     1201 atagatgcgc ccgaacttga gaaaatcatt gatgtaaaac gtcccgaaat aggcttgcag
     1261 cgtatcgaag atatctgaga tcggaatccc gagtgctttg gcccgttcgc ggttgacgtt
     1321 ggcatacaat cgcggagccg acacgcggaa gttcgtcccc acctgtccga tcgccggttc
     1381 cttcttggcg cgctccacga attcctgagc cacggcagcg aacttcttga agtctccgcc
     1441 gctggggtcc tgtagctgcg cggaaaatcc gccgaccacg cccacgccac gcagggatgg
     1501 tgcgttgaat gccagcaaga gcgcttcggg aattttggcg aactcgccat aggccatgcc
     1561 gatgagcgcc tggacgtgcg actgcggttg cttgcgttca tcccacagat gcagggggac
     1621 gaacatcgtt gcggcattgg gacctcgtgt gccgaagacg aagttttgcc ccgagagggc
     1681 gtcagtagag tgcaccgctg gattcccttg aaagaatcgt tcgatccggt caagcacggc
     1741 atcagtccgt tgtttcgacg ccccatcggg gagctggacg accacgataa aatagccttg
     1801 gtcctcttcc ggcaagaaac tccgtggcag cgtggcaaac atgccgtacg ccaagacgag
     1861 gacgaccccg aagaccagca tgacgcgtac cggccgtgcg accatctttg ccaccatgct
     1921 cgtatagcca tgttgggtgc ggccaaatat gcgatcgaac aaagcccaaa acccgccgcg
     1981 tcgttcaggc cgcggcacca gcaccatcgc acacagcgct gggctcagcg tgagcgcgac
     2041 aaagccggac accgtgactg aaatcgcaat cgtcgccgcg aactgtttgt agagggcacc
     2101 ggtgatgccg ccgatgaatc caaccggcac aaagacggag acgaggacca acacaattgc
     2161 gatgacgggc gccgtcacct cgttcatggc ccgttttgcc gcctccttcg gcgagacgcg
     2221 gtcttcccgc atatgccgct cgacgttttc cacgacgacg atggcgtcgt cgaccacgat
     2281 accgatcgcc aataccattc caaataaggt aatcgtgttg atcgagaaac cgagcgcata
     2341 gagaccggca aaggtgccga tcagggagac gggcacggcc actgtcggta taatggtggc
     2401 tcgccagctc tgcaagaata cgtagacgac gaacaccacg aggatcatgg cttccacgag
     2461 cgttttgacc acttcattga tcgacacttc aatgaacttg gtggtgtcat aaggaatgtc
     2521 atacgacacg ccggcgggga agttcttggc catctcatcc atttcggcac gagtccgcct
     2581 caccgtatcc agcgcattgg cgcccggcgc caggaaggtc agaaggaagg tcgtcgggct
     2641 gctgttccag cgtccttcca acgcgtagga ttgggcgcca agctcgatct gtgcgacgtc
     2701 cttcaacctg acgttggacc catccggcag cgcccggacg atgagttctt cgaaatcctt
     2761 gatctccgtc aatcgtcctt tggtgatgac gggaatcgtc aactccgtgc ctttgagggc
     2821 gggctcgcgt ccgatcgtgc cggcgggata atcgcggttt tgctcccgga tgattgccgc
     2881 aatgtccgtc ggagtcagac tcaaccgcgc catcatcagc ggattgagga tgatgcgcat
     2941 gctgtagttc tgctggccga acaccatggc atcgccgaca cccttgaccc ggcggagatt
     3001 atccaccaat cgcagaatgg catagttcga taaaaacacg gcgtcatggt ttttgtccgt
     3061 cgacttaagc gcgataaccg cgaccagatc aggggacatt ttcttgacgg acacgccttg
     3121 acggatgacc tccgtcggca attgcggctc cgccagcttt tcccggttct gcgtttggac
     3181 ttgggcgatg tcgggatcgg tcccgatttc aaaggtcagt ttgatcgtga cgtgcccatc
     3241 gttgctgctt gtggaatcga aatacacgag gttgtcgatg ccgggcaact gcacttcaat
     3301 cgggcgggcg accgagtcgg cgacgacctc ggcgctggca ccgggatagt cggcgtcgat
     3361 ttgcacgacg ggaggagtaa tttctggaaa ctgcgcgaca ggcagaaact gaacggacag
     3421 cagccccatg accacgatca ctagagacac gaccgaggcc atgatcggcc gatcaatgaa
     3481 gacgtgggag gtcacgcgtc acccttgctt cggcggctcg gaaggagacg ctgccggtgg
     3541 cggggcttcg cctagaggga cagccttgac cggtgcaccc ggaccgattt tcatcaagcc
     3601 gttcaccacc acgcggtcgc cggctttaat gccttgatcg atgagccatt gatcaccctg
     3661 ccagaccgag gcctggacgg gtcgtaattc aatgtggtcg tcgggcccca ccacatagac
     3721 gaacggtccc tgcggccctt gaagcaccgc gcgttggggg atcaaaacga cattcggttt
     3781 gacgtcaccc ttgaacttca ctttgacgaa ttgccctggc aagagttccc gcttgggatt
     3841 gggaatcgtg attcggacga cgcgtgcccc cgtttccgta cggagacccg gttccatcag
     3901 atccagcacg ccttcatgcg gataggtaga gccgtccaag agcatgactt cgcctcgaag
     3961 ctgatagaca cccgggtgct cgattctctt ggcttcgatg tcgcgccggc gcttcaatat
     4021 gaagctttcg ggaatgctca ccacgacata catgggatcg acccggtgca ccatggtcag
     4081 taaatcggtt tgtgccgata cgagccggcc ctcataatag cgggtacgtt cgatcaaccc
     4141 gtcgatgggc gcccgaatca atgtattatc caagtcgaac tgcgctttaa tgagatccgc
     4201 cttcgctccc tgcaaggcgg ccttggcggc aaggtcttcc gccaccgcat catccacgtc
     4261 cttctggctg accgcctgtt cgctcaacag cggtttgacg cgcgccagat cctgcttggc
     4321 ctggacgact ttggcttcgg cctgagcgat ctttgccttg gcactggccg ccgcagcttg
     4381 aaacggaacg ggatcgatct ggtacagacg atcgcccttt ttgacgtccc ggccttcctg
     4441 atacagcacc gctttgatca agcccgtcac ttgcgatcga atctcgaccg gacgagaagc
     4501 ttccgcctgg ccgatgaatt ccggctcgtc gggaaccgtc gtggtcgccg ccgtgaccac
     4561 ttcaacttga ggcatctggg gagcaggagc agagcctgcg tcctgcttgc acccggcgag
     4621 gctcgccgtc gccaggaaga cgaggaacgc cgatgtcaga agcgggtgta ttcgattggc
     4681 cgtttgcgcc gctgtcaagt cgatgggtcg caacataggg tgtttcctcg gctgcctgtg
     4741 gctatgttgg catcttagag ggctgcagac tttcttcgca agtgcggcct tcgttgctct
     4801 tttttcactt accgatctgg tttttcgcat cctcgacgat tgatctcatg caacagcctg
     4861 tggcgagcaa ctcggctccc gactgacaca ttcgactcaa gagcgccttc ccttgttgtt
     4921 caacaaacgt gacgccggtc aaatcgacga tcagttgacg gccggcagcg gattgctccg
     4981 actcacgcca gcaacgttcg agttccccga tccatgggcc tgccaggcgc ccttcgagcg
     5041 tgattctgct tgcatcgtca gacacgtggg tggtgatctt caacatgata cgagatcttc
     5101 tccttcattc ctgcatagag actcaggcct tcgctccggc gagcaatgcc tcatcatctt
     5161 ccgtcatctc cacctctgcg tcgcctaacg catccggttg gtgctcggcg gcgatcaatg
     5221 agtagaacac cggaaccacg aagagcgtga acagcgttcc gaccgtcatg ccggtcacca
     5281 gcaccatgcc gatgctgttg cgcgccgccg cgcccggtcc ggacgccagc acaaggggaa
     5341 gatgaccgaa gacggtggcc gccgacgtca tcaacaccgg tcgcaagcgt gtcaacgccg
     5401 cctcgcgcac cgccgcggcg cgtgggaggc cgctgccttg aagctggttg gcaaattcga
     5461 cgatcaagat cccattcttc gcaataagtc cgacgagggt gatcagcccg acctgtgagt
     5521 agatattgat ggtcgtcaga tccagaaaac taaagaccag cgcgccggat atcgcaaggg
     5581 gaacagagcc gagcagaacg atcagcggat cacggaagct ttgaaactgg gctgccagca
     5641 cgagataaat caacagcacg gcaaacccga gagtgaccgt gagggcaccg ccttcctggc
     5701 ggatctggcg ggactcgccc gcgtagtcaa gaacgacgcg gggtccctgg gacgacgctg
     5761 cggcggcttc aaggatgtgg agcccttcag ctttggtcac tccgggcttc acgccgccga
     5821 atatccgcac cgcgttgcgc tgctggaacc ggttcagggt gcgcggcccg gtgctcgttt
     5881 cgatgtgcgt gaaggtcgac accggcacga gttgaccggc gggcgttttg atcttgagat
     5941 cgagaagcgg ttccaccgtc gcgcgatctt tgtcaccgat ctgcggaata actttgtagc
     6001 tgcggtcaaa atagttgaag cggttgacgt aggccccgcc caagagcgtg cccagttccc
     6061 ggccgacacc cgcgaggtcg agccctaaat cagccagacg ctcgcggtcc aggaccacgc
     6121 gcgcttccgg aagatcgatt ttgaggtccg tatcaacgta caggaatttc ccgctctgcc
     6181 accccgcgcc cagcaccgca cctaccgtct ccagcatctg ctgagccggc acatcgctct
     6241 gcaacaccag ttcgacatcg tactggcccg gcgtcggaag cggcggatcc agacggggga
     6301 acacgcgtaa ccccggcacc tgcgacactg cgccgaagac ctgaccgtac atctgctcgg
     6361 tcgagcgcgc ccgttcatgc cagtccttgg caaccagacc cccgaatcca ccccaagccg
     6421 tcgtcaatga ccacgtgaat tgtgtttcgg gaaaagccgt gacggcatcc acaatctgca
     6481 gatgctggcg attggtggcc gccaccgtgg aatcgggcga tgcttcgaaa aacaagctga
     6541 tatggctttg atcctccacc ggcgcgagct cctgtcgtga gaacaggtag agaggccagg
     6601 cggcgatcat gaccagcact gccccggcaa caatacccca gcgcatgtcc agcgcccgat
     6661 cgagcaagcg agcatactgg cggcgcacca actcgaacgt ccggttgacg agcgctgaca
     6721 ttcggccttc cttcccttgc gggtgcacga atcgtgagct catgacgggc gaaagggtca
     6781 cagcgacgac gcccgacaat acgaccgcca ccgccagggt catggcgaat tcgagaaaca
     6841 gcgaaccggt taagccgccc tgaaagccga tcggcgtata caccgtagcc agcgtgatcg
     6901 tcatcgcaat gatgggaccg attagttctc gggcgcctgc cagtgccgct tcgatccggc
     6961 tcttgccctg acgcacatgt cgctccacgt tctccaccac cacgatcgcg tcgtcgacga
     7021 cgaggccgac cgacaggaca atggcgagca gtgtcaaaag attgagactg aagccaaagg
     7081 caaacataaa gagcgccgct ccgatgagcg acaccggcat ggcgacgagt ggcacgagcg
     7141 ctgtacgcac ggaacccata aagagaaaca cgaccagtgc aacgatgaga atggtttccg
     7201 acaatgtctt ggtaatttcg gtcaacgcat tccgcatgaa catcgtgccg tcccagacca
     7261 gccgcatgtc gatatcggcg ggtagtgtgg gtcttatccg ctccatttca tctcgcagcc
     7321 gtccggcgac ttcgatttcg ttcgcgccgg gaaccggcca gacaccgaga tatacgcctt
     7381 ccgtccggtt gtacttcgca accatttcag cttcttcggc ttcacgctcg acctgcgcga
     7441 catccttcag gcgcacgatg gctcccccac ggtcagccac aatgagatcc tcgaactcct
     7501 gggtcgaacg gaggtcggta ttggcgagca gattgacctg gacgagattg cccttggttc
     7561 gaccgacggc ggccaaatag ttgttccggc gaagcgctcc ctgcacgtca ccgggcgaaa
     7621 ggttgaatgc tgccaaccgg tccggatcga tccaaatgcg catggcaatc tggcgtccgc
     7681 cttcgatcgt cacgcgttgc acgccggaca gtgtggagag ttgcggctgc agcgtgcgca
     7741 gcagccaatc cgtgacggcg ggcactgtcc gctcgccgga ggaaaagctg agataaaagg
     7801 aggcgtaggg acgatccgcc cgctgcactt cgacgaccgg cggctcggct tccgccggca
     7861 attcagcgcg tacttgttga agtcgtgccg tgacctcggc caaggccgcc gtgctactgt
     7921 ggttcaattt tagatgcacc gtcaccgtgc tcacaccggc acggctggtg gactcgacat
     7981 aatccacgcc gccgatcgcg gagaccactc gctcgaccgg cgtgctgagg aacccacgga
     8041 ccgtctcggc gctcgcaccg taatagatcg tcgtaatgac caccgacgag ctctcgatct
     8101 tcggatactg ctgcaccgga agcgtcgtca gcgcacgcca accggccagg acgatgacca
     8161 ggttgacgac gatcgccaac acaggatgct tgataaacac gtcggtgaat gaacgcatat
     8221 tcatcaatga ctctcgtttc ttcatattct cagtgttgac tcaccgtttg ccgctgttcc
     8281 gactgaggag tctgggcgcc ggcaaccgcg acaagagccg actcacgaag cttgaatgat
     8341 ccggaagccg cgacctgctc accggcgatg agtcccgcat gaatgaccac ctcgtcgccg
     8401 aacatcgcgc cgctctcgac ctgctggaca tgggcgcgag cacgaccatc cgggccctgt
     8461 gcaatcacga acacctgatc cccttcgggg cccttgcgca atgcactgac cggaacggca
     8521 acggccttgc gcggggcccc caccggaata cggacgcgaa ccgaggaccc gggggacggc
     8581 gcgttgccgg aaccttcgat gcgcgctcgg accacggcat tgcgtgtcgc cgggtcaatg
     8641 cgcgcgtcca gcgcgacgat cttcgcggtg atgggtgcgg gggtacccgc aaacacctca
     8701 acgacttcac cctcccgtaa gccagcggca acctgctgtg ccaccgtaaa gtccacatgc
     8761 gcggcgtcat cgataccctg aagtgtcgtc aagagggtcc cttcgttgag atactggcca
     8821 ggatgaacgt ccgcgatacc gacccgtgac cgaaacggcg cgcgaatcgt cttgcgggca
     8881 atgatcgctt tggtgcgggc gatttgcgcc cgggcgatgt ccaggtccgc gcgcgcccgg
     8941 tccacttctt cctgcgtcgt ggccatttca tgattcagat tctgtctacg atcgagcaca
     9001 gtcttggcca gcgcagcctg cgcttcctgg gccttcaact cagcttcttc caccgacaca
     9061 tcgagcgcca cgagcaccat gcccggctca acgatctgac ccggtgacaa acgcacttcg
     9121 cgcaccgtgc cagcaagctc gtttcgcagc gtgattgact tcaacgccaa gaccgtgccg
     9181 atcgaggttg tggtttgtcg gtgctcaatc tctttggcga gggcgacggt caccgtctcc
     9241 attggttcgg gttgactcgc tgaagcagcc tgaccggctt ggatggcatc atatttccag
     9301 gctgccaggc cgacgcccat gagcagaacg attacaggca gtacgactga tccgacccag
     9361 tgacgccgac gtttcatgaa acacctccac gcttgcctga caaactgacc gcctatccac
     9421 tactgactga gaggagacag caaccttcgt tccacctctc gagcaatcat cgtccatcta
     9481 agtaaaggaa gttgcagagg acttttggca tctgctccaa agagcgctcg atgtgccgac
     9541 caccattcgc cgcctaatcg gcaggtgccg acgcatcggc atcccttcgt ttcacgccgg
     9601 acttgcagcc agcggacgga cacccggcag gaaggcctct gcgggcagaa tgttgtagcg
     9661 cctcctgagc tcctcgactt gctcgcccat gacaggccaa tagtcccagc cattattaag
     9721 atcgacggtc attgccgcac ctcgccggat ggcgatcatc gccttcaccg gatcgaaaaa
     9781 gccggttctc gccgcggtga tgggggttaa tcggatgccc acatggaatt gcagtagcac
     9841 aaaaaagacg aacgtccagg gatcccgcct ctggaatccg gcgctgaagc aggccacttg
     9901 cacctcttca tcgggtgtcg tcccataacc ggacaagacg tgcgcgcaat catggaacaa
     9961 tacctgctct gccgctccgc ccttttcacc gggcaacgga aaaccgttcg cccggcaata
    10021 ctcccaatag gaccgtccaa gagatcccgg aggatactgc gccaaggctt gatagcggga
    10081 cgcgagttga gcgtcttcat accgtcccag catgccgcgg acaaatttgt aaagtccctt
    10141 gagacgctcg tgttgccaaa tgtccttcac cttctccctc agccagaatc ggcgtgccag
    10201 atcgagacga aggcgaagca tctcgccatt gatgaggtat cggaggtcac gaacttccgg
    10261 agcatccact tgcaacgccc gggcaaattg ctcaaccgcc tccgtctcgc gcacgttggc
    10321 cttcccgtcg atcaacgaga tcacgatcaa cccttgtgtg agttgatgcc tgatctgcgg
    10381 gtcgatcaat gcggaggcca gctcctccgg cgtgacgggc gcaagatcct cgacgacata
    10441 gttggttccg agaatggtct gcaccgatgc cagcatatgc cgctccgtgt catcgagtgc
    10501 gccatccgcc aaggccacgg ttttcatcgc acgcagcccc caatacgctt gttctaccgt
    10561 gggcattttc agttccatgt gctttctcct ctgaatgacg cctagtgaag tcggttccgg
    10621 ctcatgtttt aggccggaac aatctgacga tcggatagtt gcacggccga gggcggattg
    10681 gcggccgcat tccacgctcc gaggccccca gccagaggcc atacgcgcgt gaaccccaat
    10741 ttacgaagcc tcaaaacacc ctcgacgctc gacgcgtctc tcgggcaggc gcaatacaaa
    10801 atgacatcgc ggtctcgggg gagcccatgc tggtgggtca ccatctcttc gagcgccaaa
    10861 gatacggcgc ccggaatccc cggctcctcc tggcgtgcgg caagcgaacg aagatcgaca
    10921 atcaccggtt gttctccggc ggcgagcttg tccgtcaatt gccggacggt cattcgtggc
    10981 acgccatctc ccatgcgata acgatgagcg gccttgtagc cgacatagct catggccgcc
    11041 gcgaccatgg tcagaccgat ggtcggtccg atctgggcgg tgatcgtcaa cgcctgttcc
    11101 acctgatcac tgaaaatcca tccgagacca attcctgtcc cagcccagag caaggttccc
    11161 aacccattga agaggaggta ctgtggcacc gagagaccca cgattccggc caggggcggc
    11221 gcaatcgtgc tgaagcccgg cacgaacttc gcgacgatca atgcacggac accatggcgg
    11281 gaaaagaaat cttccgtccg gcggacgcag ctggtcggtt ccaatgatat gcggcataac
    11341 cagttcaaca ccccccgacc gcgtcgacga ccgagctcga accacacctg atcaccggcc
    11401 atcgccgcga cgactgccga gacaagcgcg acgccagctg ccatctgccc ggtcccagcc
    11461 agcgcacctg cggcaatcaa gaaaggcaag gccggcaggg gaagtccgat ctgttcggcg
    11521 aagaccgcgg cagccaggac tagcgagcca tgttgagtca gaaaatctag cgtttcattc
    11581 atgatggact cccattaaaa gatcacgtcc gcctggcccg ttacacgcgg tcgttcttcg
    11641 ccagtgagat gttcctgcag ctgcacggca gcgtgccgcg caatcctgac aatgcgctga
    11701 tcatcttctt gacggccgcc gggcagtgcc gagaccagcc gcgatcggtg taactcaacc
    11761 acgacgcggc cgatgaccca ctggacactt ccaacccccg gttggagctt cacggctcca
    11821 gaggcgctgc tatcgcgttg gcgaatcagc gcatcttcca caatggcctg accgagagtc
    11881 ggctgcagcc agcgcatcga gatgtaaaga tccgccgagc ggtccttcaa gacgtctatt
    11941 gccggggcac caaaggtagt cggtgtaaaa aggaaaaata gacacaggaa gatgccggcg
    12001 atggtggcca tcatgccgat caacgtgaac agatctatgg ttggggacgt tcggttcatg
    12061 ggtcctccga tcagttcctg tgcaccgctc atgcggcgct ggtcagcaag gaccgttccg
    12121 tctcgcactg aacgcaccac gaacatcgag cagaggcaac tctgtgatct ctcagaggaa
    12181 ttgtgccgtt gtaaggtgtg cgtgcgaagc agagaagagg agagccggaa aaaggtgccg
    12241 atatgtcggc gcgatgccga ggcgtcggcg ttatccagga cggacaatac cgagtttctg
    12301 cattttcgac tgcagggtcg tgcgcttcat gcccaacttg gccgctgcac ccgcagagcc
    12361 gccaatcgtc catttcgcgt cgcgcagcgc cttcagaata tgttctcgct cggcatcctc
    12421 gagggtctta ggcgaaccgt tcgagggggg gatcgatact tttaactctg acagtgggac
    12481 gaagagatcg gtccccgccg ataagatcac tgcccgctca atgaagtttt ccagctcgcg
    12541 cacattgccc ggccagggat aggcctgtag cgccttcatg gcctcggcag gaatcgtttc
    12601 gatccgctta ttcatccgaa gcgcaaactt ctgggcgaag tggcggacca acgccgggat
    12661 atcttcagcc cgctcgcgca gcggcgggac cgtgaccgga aagaccttga gccggtaata
    12721 gagatcgctg cgaaacgtgt gctcctccac catgtgacca agatcgcgat tcgtggcagc
    12781 gacgagccgg acatcgacgc gctgagtcct ggtgctgcca agccgctcga attcctgttc
    12841 ttgcaagacg cgaagcagct tgacttggag ctccagcgga atctctccca cctcgtccag
    12901 aaacagcgtc cctcggtctg cggtttcgaa ccggccgatc ttggtcgcga ttgcgcccgt
    12961 gaacgctcct cgttcatgtc caaagagttc actttcgagc agacccgttg gaatggccgc
    13021 acaattgagc ttgacgaagg tgcgttcgcg gcgatcgctg agattatgca gcgccctggc
    13081 gatcaattcc tttccgcttc cggtttcccc cagaatcagg acagtcgagg aggtcttcgc
    13141 gaccgtttcc acgtccttca aaacgcggcg aagagtttgg ctttcaccga cgatttcctg
    13201 gaagttatat tcggtctgaa tttcctcttc gaggtacagc ttctcttcgg tcaacttgtc
    13261 tttgagctga gcgatttgtt tgtaggccaa ggcgttttcc acggcaatcg ccacctgcgc
    13321 gccgatttgg gtaaggagtt ccgcgtcttg ctcgctaaag gcttcttcgc gcatgctgac
    13381 gacgctcagt gtgccgagaa ctctcccatg agataccagt ggcacggcac atccggacct
    13441 caacccttcg gctttcgccc gcttcatgat gtcggctggg aactcggcgg tgtcgatatt
    13501 tcttaggagg acggtctgtc gagacgtgaa cgcgcgacct tccggcgtgc cctccagcgg
    13561 aatcgcatga cccgtttcga caaagctctg gttgctcgga aagtccaggg cgtgcgcgag
    13621 cagccgctgc gtgtcctgat cgaacagcgc gaatcctgcg agatcgtggg gaaccacgcg
    13681 gctgaggctg acagaaacgg ccttgaggag atcgcgtagt tcaaggtgcg aaatcacggc
    13741 attgttcact tcgaggagga gacgctgcaa gtctcgttcg cgcgccagct gctgttgata
    13801 cgctctagtc gtctcggaat tgaacgcgtt gtccacggcg accgccactt gctttgcaac
    13861 ctgttgcatg aacttgagat cagcgtctcg ataggtcctc tgctgtaaac ttccaaagcc
    13921 catggccccg agccggcgct gcgccgtcgt cagcgggacc gcgcagaagg actggacgcc
    13981 gttctcccgc aatatcgaca tcaatttagg aaaccggtgt tcctgcgaca cgtcgttgac
    14041 tgtcagcggt tcttgatgag tccagaccca tccgcccggt gactcttcca ccggcgtttc
    14101 caggcccggg ctgatcgtgc tcggcacaga agcgaccaga agccagagac gcatcacctg
    14161 acgggccggc tcgtgcagga cgatattgat atagtcgaat ggaacgatcg aaggcaggcg
    14221 ttgggctaag tcccgcaaca actcggccag atcgcggtgc gaagcaatgg actccgtgac
    14281 ctccagaagc aggcgcaatc ggtcacgatc gtgaaggagg tcctgatgat ggagcacgtt
    14341 gtccactgcc acggccacct gcttccccac ctctcgaaga aactcgatgt ccccttcccc
    14401 gtaggcatgc ttgcgtagac ttgcaaaccc catcgccccc aattgacgga cggcggtggt
    14461 gagcggcacc acgcaaaacg aattgacacc atcctgctgc atccgttcga tgaccttcgg
    14521 ccagcgaagc tcttcgccca gatcaggcac gaggatcggc tgttgcgact gccagaccgt
    14581 tccggccgga gtttcctcga gcggctcttc atggccgccg accagatccg ccggaacgtt
    14641 cgcttgaatg gtctgaaggc gcatccggtt gcccgtcgga tcatgcaagg agagcgcgac
    14701 aaaatttacc tgcaccacac ggggcagacg tcgggccaga tcccgaaaca gttcgtgcac
    14761 gtcgcggtgt gcggcaatgg cttcggcaac ctcgaggagc gcttgatacc gctctcccag
    14821 agtctcacag ggtgaggcag ggtgatccat agccgctcag tatcgcctaa ggatcacgca
    14881 tgactcaagg ggctagagcc gcagcgactt gatcggcgac aggactgcgg cagggtccgc
    14941 tttccacacc gcctcatcgg catccacgta gagctctact tcgttgccgt cggggtcccg
    15001 aaggtagagg ctctggctaa cggtgtggtc gctcattccg tcgatagtaa taccggcttg
    15061 ctcgagttcg cgcttagccg agcgcagctc atcgaggctg tcgccgacct tgatcccgat
    15121 atgatagaga ccacgacggc ggcccgaagg gggagggggc gcatccccca cctggagcaa
    15181 aagtaactcg tgatgcgtac ggccggaggt caatgcggcg gcggctccgc cgaaaatccg
    15241 cccgacttct ttgaaaccca ctaagtcccg ataaaacgcg agcgattgtt cgagatcttt
    15301 gacgtaaaac accacatggc cgaggtaccg ggctttcatg actctcctcc ttgccggcgg
    15361 ggttcagcga gcaattccac gaccgccgca tgcgcaagcg cggcatccgg gtcccgttcg
    15421 agggcgtcgt gataccgccg attgaattcc tctccatcct tcctgggcac caggagcgtc
    15481 cggagcgacg ccgtttgagc gtgaagcgct tcggcgcaag acgcgtcctt cccttcggcc
    15541 agttcgtcca agcgtgccat cgaccgcgac aggtccctca accaggcaaa tgacggatgg
    15601 ccgagcacga gctgaagaaa tgctccattg gaacatatgg ggccgtgcag gcgttcatac
    15661 gccgctcgtt caccatcgag caacgacttg tgcaaatgca gcagagcttt cgacaactga
    15721 agcacttccg agctatggcc gcgttgggtc atggtcaatc ccggcgccgc cttttcgagg
    15781 cgcttgtgaa actcagcccg accttttgaa cctcttgata agggaggagc caaaccaggg
    15841 attcatcgga caggtctcca acttgaatgc ggtttcccca cggatcccga aagtcgcaac
    15901 ggaagcctgg gtgcaacttg agtttgtact tgttcgtgag cttgcgccgg acttcgttga
    15961 tttgcttggg atcccgcacc atcagaccga agtgcttgac ccggtcaggt tgcagcctct
    16021 ccacctcgaa gatggccatg aactggtgtt cacccaactt gcaccaggcc gctccttcgc
    16081 cacctcgcag cattttgagt ccgaagacgt cggaatagaa ctgaaccgcc ttttttgcat
    16141 cggtcacttc gatcacaaca tggttgcagc cgtaaacctg tacaggcatg aatcactttc
    16201 tggtcaggtg atcttcggct tgccgcacac ggatgctttt gatcgcgccg agaaagacgt
    16261 cgaccggctg cgcccctgag atgccgtagc tcttgttcaa gagaaagtag gggactgccc
    16321 ggatgccgag gcgtcgccct gccacctcct cggctttgac gttcgccgcg ccttcctctg
    16381 tctgaagaaa ccgggcggcc ttcaggccgg cggatgctgc gacgtcggcc aggactgcct
    16441 gtgatccgat gtcagccccc cgttcaaaat aggctttgaa cagcgcctcc acgacacaat
    16501 tctgtcgatc gtgctgcccg gcataccaaa tgaggcgatg cgccaaaaag gtatttggcg
    16561 tcttacgcat tctctcaaag gcgaaagata ttccctccga agctccagcg gcggcgacct
    16621 gctcttcgag tcgctggtag gagtcttgac tgccgaactt cgcttccagg tagattcgcc
    16681 gatccatgcc ctcttcgggc atggttgggt ttaattggaa cggccgccag atgacctgcg
    16741 ttgcagccgt cgggaattgg gactgagcgc gctcccaccg tcgcttcccg acgtaacacc
    16801 atgggcagac cacatccgaa tacacctcga ccgttatact cgcattctga tcatccatct
    16861 catattccaa tcacgctcat acgatggcac aggttctcac ataccgagcc cggcgatgct
    16921 ttttgcaccc accgggcccc ggattaaatt ccggctccat ggaattactc agcatccggc
    16981 gaggacaaat gtcccatcct cccgcgctgg taatcctcga ccgcctgaac caactcagcc
    17041 ttagtgttca tcacaaaagg gccatagcgg gcgatgggct ccttgatcgg cttgccgctc
    17101 atgatcaaga gccggctgtg gtcattggcc tcgaggctga ccagcgatcc ctgtccctta
    17161 ctcaccacca actcggcttc accggccacc gcagagttaa ccgacacccg gccttcaagc
    17221 acaaagaccg aggtgttgtg tccttccggg agtgtcaact cgaaccgcgc gccagcctgt
    17281 aattggagat cgtacaattc gaccggcgta catgtgcgcg ccggtccttt cctgcctcgg
    17341 aacgtccctg caatgacacg gagacggcca gacccaccct caagatcgat agcgggaatc
    17401 gcatcattca caagcgtttg gtaggcaggc gccgacattt tgtgagcttg cggaagattc
    17461 acccacaact gaatcgctcg aaaggtgccg ccttgctttg cccactgcgc ttcatgcatt
    17521 tcctcatgga cgacccccga cgcggccgtc atccattgca catcgcctgg accgatcacg
    17581 ccggcgttgc ctgccgagtc gcgatgcgcg accgcgcctt cgtacatgat cgtcacggtt
    17641 tcaaatcctc tatgcggatg ttcgccgacg ccacgcgggt gctcggtcgg gggaaaatgt
    17701 cggggaccgg catagtccag catgagaaag ggatcgacct cctgatccag ctcattgctg
    17761 ggaaagaggt tgcgcacggg aaacccgtcg ccgaccatat gcgcggaccc tggttgatag
    17821 acgcctagca cttccttttt cgtcatcaca tccatcgtca tgatgtcctc cttctcgtca
    17881 atgcgcgatc ctttctgcaa ggatgcgatc gacagaccag gcctcctcct gtcaccagca
    17941 aggtcactgc gatgccaacg accagaaggt aatacgcgag ctcttcccgc tgtgctgttc
    18001 gaaccaaatc atgaaaaccc cctgctgcag tccaccatga caatggctgc tcccagcatg
    18061 ataacggcgg aaccagccgc cgtgaaccta gtgagaaacc cggcaagccg ggcgcctctt
    18121 cgtcgtggtt ctggtgctat cgagcaagat agttctagtt agaaccaaag tcaagtgggg
    18181 ctaatcgagc gtctggcggt agcgctttat tcccactgct aatgcgacca ccgcaaacaa
    18241 ggcgatttgc cacagctgga gggcgatctc atgaagcccg ttccctttta acaggatgcc
    18301 ccgaacgacc cggagaaaat gagtcaacgg cagcaactca ccgacggcct gcgcccattg
    18361 gggcatgccc cggaagggga acatgaaccc tgacaagagc agggacggca agaagaagaa
    18421 aaatgataac tgtacggcct gaagctggtt ttcggccagc gtggaaaacg taatgcccat
    18481 ggcgagattc gccgtgatga acaggaaggc gaccagcagc aggaggccaa gattgccatg
    18541 catcggcacg tcgaacagga cgcgcgctgc cagcaggatg agcccgagtt ggacgtagcc
    18601 gacaagaata tatggaatga ttttgccaac cataacttca aacggtcggg tcggcatcga
    18661 cagcaagttt tccatcgtcc ctcgctcgcg ttctcgagtg atcgccaagc cggtgatcat
    18721 caccatcgtc atcgtgatga ccacccccat gaggccggga acgatattgt attgcgtgat
    18781 cgcttccggg ttgtactgcg catgcacgcg cagctcgatg ggatcctccc gtcccgccag
    18841 gaagacaagc ggacctttga gatcgtgctc gagcgccttg gtcaacacca tccgtaggga
    18901 accgatggca ttactcgtgg ctgccgggtc agtcgcgtcg gcttcgatca gaatcgccgg
    18961 ccgttcgccg cgcaacagct cgcgcgtgaa gccctgtgga atgttcacga cgaactgcac
    19021 gtctccgcgg gcgaggacgt cacgaccttc ctgttcgctc gtcaattcgc gcgtgaactc
    19081 gaaatagccg ctgttccgaa tcgcatggag cacagtccgg ccttgtgggc catagtcggc
    19141 catcaggacg gctgtaggga ggcgcttggg atcggcattg atggcatacc cgaacagcac
    19201 cagttgaatc agcgggacgc cgatcatcat gccgaacgtc acacggtcac ggcgcatctg
    19261 gatgaattct ttcacgacga ttgcccagag gcggggaacc gagaacatcg gcgacatcct
    19321 tacgacgtaa agttgtcagg cgtccgattc atcagatgga taaacacttc ctcgagtccg
    19381 gagctgatag gacgccattc gtatggcggc ttgcgaaacg agaggatggc cgcctcgagc
    19441 gccttctcat catcgctgct gacgtgcaac cggttgccga aagccaccgc ctgtcgcacg
    19501 ccagaatgtt tccgcagttc ctcggcgagg tggtggaggt ttggtccgct gaccgaccag
    19561 gtcgtgaggc gcgcttgggc aatgacctcc gtcaccgttc catgtgtcag caaccgcccg
    19621 taggcgatgt aggccaggcg gtgacatcgt tctgcctcgt ccatatagtg ggtcgtaatc
    19681 aggaatgtca ggccctcggc ggccagttcg tgaatctgct cccagaattc acgccgcgct
    19741 ttcggatcca ctccggcagt cggttcatcg agcaacagca gctgcggttg atggatgagg
    19801 caggctgcca atgcaagccg ctgcttccag ccgccggata actggccggc aagctgccgc
    19861 tggcgtccag cgagcccgag ccgttcgagg cttctggcga cggcctcccg acggttcttc
    19921 acggcgaaca tccgggccac gaaatcaagg ttctcggcga tgctcaagtc ttcgtagaaa
    19981 ctgaaccgct gagtcatata accaacttcg gcctttattg attcgctgtc gcgtacgacg
    20041 tcgtacccca agcaggtgcc gctgccctcg tccgcccgca ggagaccgca gagcatccga
    20101 atgaatgtgg tcttgccgct gccgttcggt ccgagaaaac cgaaaatctc cccgcgcctc
    20161 acttgaagcc ctatgtgatc gaccactgtg cgctcgccga accgtttggt catgccatgc
    20221 acgtcgattg cgagtgcctg tcccgtcatc tccctcctcc tgaggaagac tcaaaccgga
    20281 catccaccgg ctggcccgga ttgagattga ccgcgctctg aggatcaaac accacttcaa
    20341 tcatgaatac caatttgtcc cgactttcct gactataaat gaccggcggc gtatactctg
    20401 cgcgcggcga gatgtagctg accgtgccca tgaacggttg gggcacacta tccacggcga
    20461 cctggacatg gtcaccggga cgaatcgttc cgactcgagt ctccgagacg aacgcccgta
    20521 ctttgatatt ttcgggcggc agcaggacga cgacgggtcg cccggccggc acccattcgc
    20581 cctcccggta caaagtgtcg aacacgagtc ccgatttcgg agcctgctga cgcttctgcg
    20641 cgaactccca atccgctcga gccagcgccg cttcgcgggc gtgaacctcc gcttcagcgg
    20701 caatgacctg atcggtgcgc gaaccgagta aggccgtgtc caaatctgcc tggagctgtg
    20761 ccacccgttg tcgatcctgg tcccgagccg cacgcgctcg atccacctcg agctcgaccg
    20821 ccgcgccggg cacctggctc aacccttcct gccgcacgac ctcccgcgtg gagagccgca
    20881 aggcagcctg cgcgtgcttg agctgcgcct tgagggaatc gatctcagac gggcgcttcc
    20941 ctttttttgc atcttcgaca ttcgcgaggg cctggccaag ccgtcgctcc gcttcatccc
    21001 tcgcggcttt ttccgcgcca cgctccaagg caaacaaggc gtctcccgac cgcacttgcc
    21061 tacctcgctg gatcgacagc gattccagcg cgccggcaaa cggcgaggca acgtagacga
    21121 attcgccttc gacatagcct tggactcgat cggacggcgt gcgttcgcaa gcaaacagga
    21181 acccggccgt catcatgacg atcggcagga tgaacgcctg acgccatgtc gttccgacac
    21241 gaccgatcct catcccctcg gcgggtccgc ctgcctctag aacacgatgc gtttccagcg
    21301 tcaggcggag ctcctatttt ctttctttga tggctcgttg cacttcccgg ccggcttcac
    21361 gagctttgat cgattcgcgg cggtcataga gctttttgcc tttggccagg ccaagctcca
    21421 ccttggcata tccacgcttg gagaaataaa tgcgcagcgg gatcagcgtg agacccttct
    21481 gctgcgtctt gccaagcagc ttgttgatct ccaccttatg gagaagcaat ctgcgcaccc
    21541 tcgtcggatc gtgattcatg atgttgccat ggctgtatgg actgatgtga cactgatgca
    21601 gatagacttc cgtgccgcgg accgaggcgt aactatcttg caggttcact cggccctccc
    21661 gtaaggactt cacctcagtg ccctgcaaca taatgccggc ttcgtatttt tcctcgatga
    21721 aatagtcgtg atacgctttg cggttggtcg ccacgacttt tccctctgct gcgttctcct
    21781 tggtcatgat tcagtcggca ttgctccctt gacccgttcg aagccgcagc ctatcattcg
    21841 gcatgcgccc ttccggttcg ctcgattcgt tcagcatcat tctctcgggt ttggcaaaac
    21901 gcttgggtct ccaaacccat ctctttgaag tgcgccttca gcaacaatgg cgtgagatta
    21961 tcggcgaacc agtcggctcc catacctggc ccgtacaggt tcggtttaaa aaactttact
    22021 tgatcgtccg aaactcggtc tggttgcagc aactcatgtt cttgaagccg actctgctcg
    22081 ccaaggtgaa cgagcaggca ggatcggaac tgatcacgga tatcgccttc cgcgtgggtg
    22141 aaatccccga tgcccggacg gccgcgccgg cttcgcagcg ccagcaacag ccagtgaacg
    22201 aagccgccgt ggtcgaagcc aacgcccatg cagccgccgt acaagatccc gagctgagga
    22261 accggttggc ctcgttaatg gctgacgcct tttcccgccc ccgctcttaa ggctgctcga
    22321 ggaggctgcg cttgaacaat tgccgagaat aagcggccgt cgctgcagaa tccggtccgt
    22381 ccacgatcgg accgattcga ccctcttcgt cggtttccgt caatcgatac aaacctcgta
    22441 gcgcacgctt gaatccgtcg cgcacttcct ggtcctcacc tgtcagatac ttgtggagga
    22501 tcggtgcctc ggcccacgcg tcgtgcgtga agatgtagat cgtaatgcgc cggtagtgtc
    22561 ccgagggatc gtccggcgtc gtcggcctga tcttcatcgg tccgaagttg cgtgtttccc
    22621 gcccgacttt tcgaaagcgt tcaatcaacg tttcgatctc catatcgctg gtccaggccg
    22681 gaacatggac cgagacggca gtatcttcct gggagccgat actataggga ggaatggatc
    22741 gatccggcct gctgaggtac atcccgcccc aaatcagcgc aaaggatccg aaaaagacca
    22801 tcagggcgag cttcacgacc gtatcgagcg atttcttcgg cggggaaagc ggcggagact
    22861 gtgacgtggc gttgttcatc ggagcggacg atcaccgacc gacgattatt ggccagccgg
    22921 gccttcctcg gggccggctt cttcccgctc gacggcgtcc gcgagacgag ccaccgccac
    22981 gacacgatcg tcgtctccgt ccaggtcgag gatccgcacg ccttgcgtgt tgcgaccgat
    23041 ctctcgaatg tcgccgactt tgcagcggag caccttgcct tgcgccgcca tcaacatgat
    23101 ctcatctccg tcccggactt gcaaaaatcc gacggcgagg ccgttgcgct cggtagtttt
    23161 tacgctgatg atgccctttc ctccgcggcc ctgaacgcgg tattcgccaa ccggcgtccg
    23221 cttgccatat cccccctcgg tcaccgtcag aatggccgtc gtggaatcgg gcgttatcgt
    23281 ttccatgccg atcacttcat ttccctcttc cagcgtgatc cctttcacgc cgtaagcggt
    23341 gcggcccatc ggacgtacat cgctctcgga aaacctgatc gtgatgcctt gcttggtgcc
    23401 taaaagaatt tcccgctgcc cgtcggtgac ttgtaccccg atcagcctgt cgccgtcttc
    23461 caatccgaga gcgataatgc cgccctgccg gggattgcta taggcagaca attcggtttt
    23521 cttgatcaca cctttcttgg tagccatgac cacaaaccgg tcagcagggt agtcctttac
    23581 cgccaaggtc gctgtgacct tttcgcttcc ggccaaagcc agaaggttga ccagcgcctt
    23641 ccccttcgcg gctcggctgg cttcaggaat ctcatggacc ttcagccaga acactttgcc
    23701 cgcgtcggtg aagaacaaca gggtgtcatg cgtggaggcg gtgaggagcg tctccacgaa
    23761 atcttcgtcc ttaatcccca tgccgatctt ccctttgcca ccgcgccgtt gagcacgata
    23821 gagagacgcc gcgttccgct tgatgtagcc ggcatgggaa attgtgacga cgacttcttc
    23881 ctcggcgatc aaatcctcaa ggctgatttc ggcttcttct ttgacgattt gagtccggcg
    23941 gtcgtcgcga taggcttcgc ggagctgggt cagctcgtct ttgatgatcg accgcacgag
    24001 cccttcgctg gccaacaccg acttcagata ttcgatttgc ttcagcactt cctggtattc
    24061 ctcgaccagt tttgctcgct ccagttgggt cagccgctgc aagcgcatat ccaggatggc
    24121 gttcgcctgg atctcgctca gcgtaaattg ccgcatcaag ccggtgcgcg cttcatcagg
    24181 cgattgggaa cgccgaatca aggcgatcac ggcgtcgagg ttatcgaggg cgatcttcag
    24241 accttcgagg atgtgcgccc gttcctccgc cttgcgcagc tcgtacgtgg tgcgacggac
    24301 gacgacttcc cgtctatgct cgaggaaatg atgcaggatc tgcttgaggt tgagcacctc
    24361 tgggcgattg ttgaccaacg ccagcatgat cacgccgaag gtcgtttcga gttgcgtatg
    24421 cttgtacaag ttgttcagca cgacgagcgg aatctcgttg cgtttgagct cgatgacgac
    24481 ccggacgccc tcgcgatcag acgattcatc gcgaagatcg gaaatgccct tgatacgatc
    24541 ttcttgaatc aattccgcga ttttctcgat cagctttgcc ttgttgacct gatagggaat
    24601 ctcagtgacg atcagacgtt cgcgctcggt tcgttcatcg gtttcgacga cgactttcgc
    24661 ccgcagggcc agcagcccgc gacccgtttc ataggcctcc ttgatgccgc tcatgccgta
    24721 aatgaagccg gccgtcggaa aatccggtcc cggaatcttt ttcattaact gcgcgaccgt
    24781 cacgtccggg ctctccagca gcagcaggag gccatcgatt acttcgccga tattatgggt
    24841 ggggatattc gtggcgtagc ccacggcaat cccgccggcc ccgttgatca aaaggttcgg
    24901 caccttagtc ggcaggacca agggttcctg ccgggactcg tcgtagttgg ggccgaagtc
    24961 gacggtctct ttgtcgatgt cggccaacat ctcttcggcc agcttggtca tgcgcgcctc
    25021 ggtataacgc atggccgcgg gcgaatcgcc gtccattgat ccatagttgc cctggccgtc
    25081 caccaagggg taccgcatgt tgaaatcctg cgccatgcga acgagggtgt cgtagatcgc
    25141 cgaatcaccg tgcggatgat aattgcccat gatttcgccg acgatcttgg cggacttgcg
    25201 gtaggaccgg ttgtggccga ggcccatctc gttcatgccg tagagaatgc gacgatgcac
    25261 cggcttcagg ccgtcgcgca cgtcggggag tgcgcgtccc acgatcacgc tcatggcgta
    25321 atcgagatag gaggagcgca tctcgtcttc gatcgcgatt tggcctaggc gttcatcagg
    25381 cggcatgtcc ttctccaggg atgctgaaaa tgatcacggg cttcgtccct cgcctcgcct
    25441 cagaggttca acgtacgaga cgatgtctgc tgcggccttt ccaaatgacc gctttgagca
    25501 ttattacaga ccaatcggtc ggcttgatta ttcgctttcg gcctcactac acatcgagat
    25561 ttcgaacttc gagggcatgt gtttggatga agttcctgcg cggctccact tcgtcgccca
    25621 tcaaaatcgt aaagatctcg tccacgccgg tcacgtcttc gagtgtgacc cgcaataacg
    25681 tccgtgcctc cgggttcatg gtcgtttccc agagttgccc gggattcatc tcgcccaacc
    25741 ccttatagcg ctgaatcgaa agtccctgct tgcccaagtc caagatcgtc ctcactagat
    25801 ccgaggtcga tctcagctcc tgctcctcgc ccttggcctt cagtttatag ggggctcgcc
    25861 cgaggccgat cgccgaaggc gcgagtttct gcagctcgcg gaaatccgca gagccgacca
    25921 gatcatgggt gacatccaac ccaaaagacg agccgttggc ctgcatgcgg caggacactt
    25981 tgttggaatg atgttcttcg tcagacaaga tctcaagggt ggagtcgagc ttggggtaca
    26041 tgacccgaag ggtcttcttc acgttggcga ctgcattggt aagggcagct ccatctttga
    26101 ggagatcccg gtcgagacca ggttcatcca cgaaggcgcg gatcatcgac gcttcgtgcg
    26161 gcttcttatt aaaacgcgcg aggagggttt caaacgaaat cattttcttc aggatgggga
    26221 ggagccggcg gccggtcacg aaatcctgag cgccttcgag gtacaggtgc acatcttcga
    26281 cggccaggtc ggccaaatgc tcattcaatg ctccttcgtc cttcaggtag cgctcgacct
    26341 tgcccttctt gactttgaac aggggcggtt gagcaatgta cacatacccg cgctcgagca
    26401 gttcgggcat ctgccggaag aagaacgtga ggagcagggt gcgaatgtgg ctgccgtcca
    26461 catcggcatc ggtcataaga ataattttat ggtagcgggc cttggcaatg tcgaacgtat
    26521 ctttgtccgc cttatcgctt tcttcgcgct ttctcccgat cccagtcccg agcgccatga
    26581 tcagcgtccg gatctcgtcg ctcgtcaaca tcttgtcgaa acgggccttc tcgacgttga
    26641 gaattttacc ttttagaggc aagatggcct ggaacttcct gtcgcgtccc tgtttggctg
    26701 aacccccggc cgaatcacct tcgacaatat agagctcgct gagcgccgga tccttctcgg
    26761 agcaatcggc gagcttgccc ggcagggagc cgccatccaa tgcgctcttg cgccggatca
    26821 gatctttggc tttccgagcg gcttcgcgag cgcgggccgc atcgatggcc ttcccgatga
    26881 tcttgcgagc gaccgtcgga ttttcctcga aataggcgcc cagcttctcg tttaccgccg
    26941 tctcgacgac gcctttgact tcgctgttcc caagcttcgc cttggtctgc ccttcgaatt
    27001 gcggattgcg gaccttcacg ctcaccacgg cggtgagtcc ttccctgaca tcgtcaccgg
    27061 tcagcgactc ggtctctttt ttgagcaact cgttggcgtt ggcgtaggtg ttgatcgtac
    27121 gggtgagcgc agccttgaat cccaccagat gggtcccgcc ttctctcgta ttgatgttgt
    27181 tggcgaagga gaaaagattc tcggcataac tgtcgttgta ttggagagcg acttccagaa
    27241 tcatctccgg tttctcgacc ctgacataga tcggcttgtg taacggcgtt ttggcttcgt
    27301 tcagatgttc gacaaaagag acgatgccgc ccttgtagag aaacacctgt tccttggcgg
    27361 gttcctttcg ctcgtccttc agcgtaattg acaagccttt gttgaggaac gccagctcgc
    27421 gcaagcgctg ggcgaggacg tcgaagctga actcgagggt ttcaaagacc tgcccatcgg
    27481 gcctgaagcg gaccttcgtg ccgcgacgct tggtaatgcc ggtgtccttc aagggggcgc
    27541 cgggcttgcc acgctcgtac cgctgctcga acacctgtcc atcctgccag atctccagtt
    27601 caagccattc ggatagggca ttcacaaccg aaatgcccac accatggaga ccgccggaaa
    27661 ccgtgtatgc gccctgctcg aacttgccgc cggcgtgcaa gacggttaat gccacttcgg
    27721 cggccgactt cttttgcgtt ggatgcatcc cggtggggat gccccgccca ttgtcgacga
    27781 ccgtgacgct cccatcgata tggatggtga cttcaatcgt ctccccgaag cccgccatgt
    27841 gttcgtctac gctgttgtcg accacctcat agacgagatg atgcaggccg tcgacgcccg
    27901 tgctgccgat atacatcgcc ggccgcttgc gcaccgcatc gagcccttcg aggactttga
    27961 tctggtccgc actgtagctg tcggatttgg gcttggtcgt ctcgtccgtg gccattacgc
    28021 tcctctactg caaacatccg ggtatctgac aggatgttca aaacaatcac gtgctcgcga
    28081 aaccaacttc ccggggccgc gcacgtcgtc ccgcggcgag aacgcagcga ggtccgcgag
    28141 gcgaagcatc atgagcgtca gtttgcggac gggcgcgagt tggtgaacgt ccggtgtctc
    28201 agaggcacgt cgtccatagt ctaaaaagtt gaacctctga gcggcgcgag aacaatgctg
    28261 ggagtctttt tctaacatcc tgctaaattt tgatgggcat cacgacacac ttgaacccgg
    28321 gcgtttcggc ctcttggatc aagcaggggc tgagtgcact gtccatctgc aacgaaattt
    28381 tctccccgtc catcacgtcg aagacgtcga ggagataacg ggcattaaag ccggtgttca
    28441 atgcttcgcc ttcgtattgg gcggacattt cctcagtggc ttcaccgtaa tccgggttgc
    28501 tggaaaacaa cgtgagccgc ccgggagcaa acgacagttt cactgcatta gccttgtcct
    28561 tcgaaagcac cgagaccctg cgcagcgcgc tttcaagttc cgcgcgcacc accataattt
    28621 tcttattgct ctctttcgga atgacctgtt ggtagttggg atagttgcct tccatcaacc
    28681 gcgacgtcag gagcaggcca ctcttgcgga agatcatgag attcttggta aagccgatca
    28741 ggggctcgcc gtcacctcct tcttccaaaa gacgacgcat ttcttgtgcg gctttcttcg
    28801 gaatgatcgc cttgatctcc tgtggcgcgc ctttggcgcc ggctttcccg agatcctgct
    28861 cagccaccgc caaccgatgc ccatcggtcc cgacgagtct cagcgtcgtt ttcttttccc
    28921 cggagatcag cgtgaccagc aagccgttca ggatatagcg ggcatcgtta tctcccgctg
    28981 cgaacagtgt tttgcggatc aactccaaca ggcccgcccc ggcgaggggc gtcaaccctt
    29041 cacggtcgat ggcgggaagc gctggataat ccgtgctggg aaggccgacg accttgaact
    29101 ggctcttccc tgcttggatg gtcgtccagt tattttcgct ggaaatcaga tcaatgtcac
    29161 cgctcggcag ttccttgatg atttcgtaca gctttcgcgc cgagaccgtg atcccgccgg
    29221 ctttatggac ggtggccttg tagagtcctc tcatgccgat ttcgaggtcg gtggccacga
    29281 tctcggctcc ttcctgtttg gcttcgatca ggatattgga cagaatcggc atcgtattgc
    29341 gcttttcgac gaccccttga acgcgttgaa gccctgtgag caactcatct cttccaatcc
    29401 gaaccttcat aatcgcccct ctctcacccg gtcaccctct gaggatcttc tccttgagtc
    29461 cgtccaacgt ttgattcaac ccgctgtcgt tctcttttgc tttcatgatt tgccggcacg
    29521 catgcatgat cgtcgtatgg tctttcccgc cgaacaaccg cccgatttcc ggaaaagacg
    29581 cgtccgtcaa ttcgcgacac agatacatgg caatctgcct cggatggacc agcgttttgc
    29641 tcctgcgccg tgacttgaga tcggcaatct taatatggaa gcaggcggcg accgcttcct
    29701 gtatgtcctc catggccaca attttcttct tgtcgccgat caaatcgcgc agcacgttcc
    29761 tcgccatctc gagggtaatg gactgcccgg tcaatgaggc atatgcgccg aggcgcacca
    29821 gcgagccttc cagttctcga atgttgctct tcatggtcgt ggagaggaac tggatcacat
    29881 cttccggcag ggtcactcct tcatcctcgg acttctttct gagaatcgcg attctggttt
    29941 ccacatcggg gggctgcagg tcggcgatga gtccccactc aaaccgcgac cgtaagcgtt
    30001 cttcgatgtc aggcatgtcc ttcggaaacc ggtcgcttga aaggacgatc tgcttgtgcg
    30061 cctcgtacaa ggcattgaac gtatggaaaa actcttcctg cgtgcgctcc ttccccgcca
    30121 aaaattgaat gtcgtcgatc atcaacatgt cgatatgccg gtatcgcttc cgcaggtcca
    30181 tcatcttgtc gtagcggatg gagttgatga cctcattcgt gaactgctcg gtcgtcagat
    30241 aggcaatgcg cagatcggtg cgctcggcga catggttggc gatcgcgttc aacaggtggg
    30301 tcttcccgag tccgactccg ccatagataa agagggggtt gtaggcttta gctggctgtt
    30361 ctgcgactgc catacacgca gcgtgcgcga attgattacc ggcaccgacg acgaaattct
    30421 taaacgaata cttcgggtta agctgtatcc ctcgcttgga tctaggggtc gtagcggtcc
    30481 ggactgcttg cgcaactgct gcaacgtcgc tctggctgat tggaggtcgg ctaaagacga
    30541 caaaggacac ggccgtctct cctccgcccc gagcagtcga cacggcctcc tcgaggagtg
    30601 gtccgtaatg ctggctgagc caatcgccaa aaaacttatt aggaacacct atttgcgcac
    30661 ttctgtcttc gattcgctct aggcggacgg gcataaacca ggtgtcgtag acctgttttg
    30721 gaacccgtcc ctggatgtag gccaaggctt cctgccagac tgtagaaact gtcataattc
    30781 ctgaactctc acccatacac aggcttattc acacctgtgg ataagtatgc ttgatgctct
    30841 cgcttcttcg aagctcagcg atgccatcag ccagtggtgg tttgtaatgt gtctcttcat
    30901 cgacggatct caagaagtta tgcacagtgc accgccctct tactccgagt caatccccaa
    30961 ctccgtcccc aagacgccct ctagaagagt acgtccctaa tcaaccattt agcaccaatc
    31021 cccaccatcc accggtccta ctccgaatcc tacgacggcg actatctctt tctttgccac
    31081 actatcttag gagaagagcc tcgtttctaa atgacgatcg ctgcatcact cgcgaaaatt
    31141 tttttcacca gaacatcata ggagatggtg cgatccccat cctcagtgcc cagtgagaag
    31201 actgttccgc cggaatgagg aaatggaacc agggcacgat caaggagcac cacatctcct
    31261 atcgagtgtg acgcgataaa caatgcagga tccacacgat ccaggggttt tctgagtaga
    31321 tacagcgtgt tcttcatgaa aagaccaggg tgcggtcgag tgagtgcacc agcgtgcgaa
    31381 tttcgtccgt cggtttactc gttacggaaa atccgtcgag aaacttctgc attggtaagc
    31441 ctgatgccac gacgaacggt acttggagtt gctggaatga gggacgatac ttttcaagaa
    31501 tatcgacgtc gatgacgtca tctgtatcat cggtcaacag gagaggagct tttccaagaa
    31561 gaatgatggt cgtctcatgg tccccggcac aaaggccaag cgcaatgcgc agggcctcga
    31621 cgggtcgatg ggtttcggta gggtcttcag cgatgacgac ggcaactcgt ttggacatgt
    31681 cgctcgttct gctaggtgaa tgcgagaaac cgatcacatc cgtcgattat tcctgagagc
    31741 acgacaaggc cagatagcga gacacgcggg tctacctggt cggttgagac gccatgttgt
    31801 tggcacccat aggcacagac ggacattttg acacccgcgt ctaggaggcc tctgaaacgg
    31861 gtatccatgt agcttcttac gccctcatca atgagataca aatagacctc aacgccttgc
    31921 cggagtgcag cttccgccag ttgcaccacg gtctcgacgc tcggatgaga gggggctgtg
    31981 gataacagga gaccgagttt ctgttgtgcc atggaaatgt ctgtgaattt aacacgaaga
    32041 tctattaaga atcaattggt tacataagtt aatcgtgccc gagcctacgg ggttttcgaa
    32101 aggaagtcaa ttggaaaaac ggggtcaaaa agtcttgctg agggccacga aatagcgagg
    32161 aagatcggtc aatggaatgg tctcctgcac cttggattcc atatcacgta cgacagcgga
    32221 tccagctctg gcttcatcat cacctaacag gacggcaaac cggcagcctg cacggtctgc
    32281 ctgacggaga tgggctttca atgtggtggc ccggtagtcc gacaacgctg agagaccaac
    32341 ttgccggagg gcatccagta agcggacgcc agctggactg ccttcggccc cgaacgaagc
    32401 cacatagacc gagggacgaa gggtcggaac acgttcggaa ggaagcatca ttgcgatgcg
    32461 ttcgaggccg gccgcgaatc ccacagcggg tgtaggctgc ccgcctaaga gttccaccaa
    32521 cccatcatac cgccctccgg ctcccacggc attctgcgcc cccaggtggg atgacgtaat
    32581 ctcgaaggcc gtcaggcaat agtaatcgag tccacgcacg agccgtggat tcagacggaa
    32641 cgagatgccg agtgcggaaa gtgtcgagag gacctgatcg aaatggtcct ttgcctcggg
    32701 agacaaatat gaagtcaggt gaggggcatc ctcggtggct gcccggcagg caggcacctt
    32761 acagtccagc acccgaagcg ggttcgtgtc gattcgtcga cggcaattct cgcacaattg
    32821 acccactcgg gtttgcaaaa acgcgacgag gaccggaaga taagccgctc gatcggtact
    32881 ggttcccagg ctgttgatct caagagtgag atcaggcaaa cgcagatcag agagaaagcg
    32941 ccataacagg gcgatgacgt ccacgtccgc cttaggatcc tgggtcccca ggtattcgac
    33001 accaaactga tgaaattgac ggagtcgtcc ggcttgcggc cgttcatggc gaaacatggg
    33061 gccgatgtaa aaatattttt gcgggagcgg atcagcccct cggtgatgtt cgatgaaggc
    33121 acggacggtt cctgcggtgc cttccggtct gagcgtgaga gacgttccgt cacgatcggg
    33181 aaacgtgtac atttcctttt ccacgatgtc ggtggtggcg ccgatgctgc gggcaaacag
    33241 cgtcgtgact tcaaaaatcg ggatgcggat ttcgtgaaag ccgtaccgat ccgcccactg
    33301 acgggcggtt tcctcgataa accgccagcg cggcgtctct tctgggagaa tatctttaac
    33361 gcccttgatg cctttgatca ccggtgcatc cagctgtatt gatcgcgtcc tgaaggtgcg
    33421 cgcgactata gcgacgagtt tgcgggcaag tcaacgcgac gaaggcttct tgcgccgagc
    33481 ttccggcggc gctatagttc gcaacctccc gacttccgta caacaggagt ctgtgtatga
    33541 gcagtgatcg gccaacccgc aggatcatcg ccgtggcgcc catgccgccc gagaagtccg
    33601 cgtatgcgct ggcgcgctac agtcgttctc ctgactctat agaacagagc atcaaatggg
    33661 tacacgggca ttcctcggaa aagttctggg agcagtttta cttcgactat gggcatgcct
    33721 cgatcgccga tctcggccac gtcattatct gttttgaaga gatcaccgag cttgccgcca
    33781 tccggttgga agatgaaccg gtgtgggacg ggcaggccaa atcgagccgc tatcagaact
    33841 ttgcctcggg tggctggtac gtgcccgacg ccattcatgg aagcgagacc gaagggacct
    33901 atcacggcat ccttcgcagc ctcggggaaa tctatcgcct ccttcacgaa ccccttgtga
    33961 cgtatctgtc cgagcgagaa ccgcggccgc cctcgatgaa gccggatacg tatcagcgga
    34021 cgattgccgc tcgcgcattc gacgtcacac gctacctctt gccgctcgcg tgcaaaacaa
    34081 atgtcggtca ggtggtaagt attcgaacgc tcgaaaagca gatcacgaga cttctgtcct
    34141 cccagctccc ggaactccgg atgatcggcg aagacctgaa ggatgcgtgt cagcgtcctc
    34201 cgatgaacgt ctggggcgag ctgtgcggcc aaaccgccgg cctgaatgaa ccaatggctc
    34261 caacgctctc tcgttatgcc cgggcgaacg actatcaggc caccgtctac acggatctgg
    34321 cccgctatgc gaaggaagca ctccgcggca cgggactcga ccatgcagat acctggggaa
    34381 cttgcgaccc cgtcgagctt atcgatccgc acgatcccat tgatgaaacg gtcgcgacgc
    34441 tcctgtatcg tgtgagccac gcgccctacc gcaggatcct cggtgtggta cgtgattgga
    34501 cggaaaagca aaaacaggac acgatcgccg tcgcactgag ccgtcgcggg tcgtacgatg
    34561 aacttccgaa ggagttccgt agcgggtatg cattcacgtt cgatgtcctc atggatatcg
    34621 gcggctggcg ggatatgcac cggcaccggc gctgccaaca gatccaacag aatttcacca
    34681 ccgcgcatgg gtatgatgtc cccgcgcccc tgatcgaggc acgtcttgac ggtgaatatc
    34741 ggcaggccat ggatgccgtg agggcggata ttgagcagct gaagaaagtg aacggagagg
    34801 cggccctcta tgccatcccc ttcggattca aggtccgctg tctgttcaag atggattttg
    34861 cggaagcgga atatatcgct aagcttcgtt ctggggtcaa agggcactgg tcgtaccgga
    34921 acatcgcctg gctgatgaaa gagaaactca cccagcgcta tcccatgctc ggcgtgcatg
    34981 tgcaggcgac ttcgccggat atcgaagata cgctgaccag atagcgcgtt ccccttccat
    35041 gaccgagcac caaatcagct gcgaaacggc cctgttggat ggagactggg aacaggcggc
    35101 gcacgaggcg ctcgcttggg cgcgtcatcc gaaatcaacg gatcggcggg atcctcgacc
    35161 acatttcgcg cttaacgtgg tgcacttgat caaagggcaa tttgctgaag cctggagcag
    35221 ccacgcgctc tcgctccaag agcccgacga cattgctcaa gtaaaaagct ggctcgacgg
    35281 actcgtggcc cgccatgccg atcaacccaa tgcccatctg gcgatgggat tgttcctgtc
    35341 ccaatcgggg caatctgaac aatcgatgca atcctaccga acggcagcga agctggcacc
    35401 gcaatcggcc tatccgcatt attttcttgc gcagattcat gaacgtgcaa accacctcga
    35461 gatggcgatc aaagaatatc gggaggcggt gaggctcaac ccggactatg ttcctgccag
    35521 aaccaacctc ggggtggcct accaggagca aggacgattg gagatggcca ttccgcaata
    35581 tcgggaagcg atcaagctta acccgagcga tgccgtggcc catgcgaatc tggcctgcgc
    35641 gcttgccgaa caggggaaga tgcaggcggc gctcctcgaa tatcaggagg cgctccgatt
    35701 gaacccgcgg gatgcggaag tgcactttgc gctgggaggg ttgtatgaaa acaagaatcg
    35761 tcccgacctc gcgatgaaag aatatcgggc cgcgatcgaa tcgaatcgcg acctggcgcc
    35821 ggctcattcg gccctcggct ggatgctgtt cgatcgcggt caccgggaag aggccatgga
    35881 atgtttcaat caggccctgc gtgctgatcc gcagaatgcg cgggcctatc tgggggtcgc
    35941 gaagtgctat gcaagtcgag gcaaggtgca gctggcgatt gagaactata cgaaggcgat
    36001 caagtacgag caggacccgc agaaaaaaaa cgagatcatg aataatctct acaaagaagg
    36061 cgggacttgg gacacgtgag aggatggctg gggcgggcga cggcgatggg ttagaccgct
    36121 acagttcgcg gatggcgcct tggaggacgg tgatctgcgt caccgggtcg cccgaactct
    36181 tcaactcttt gcggatttga tctttcaggt aggacgaata gccgacacgg tcgataagga
    36241 gatcgagaaa ccgctcggca ggaagtaaac tctgtgcttt aagctcttca atggtttcat
    36301 cgcttacggg cacatacgtg ctgccgagat ggaacaccac atcataatag cggccctgac
    36361 cgatgcgttc gaaccgaagc cccttcatga acatatcctc attgagccag cagcaaggtg
    36421 ccattgtaga caacttgctg ccgtgaagac aagggcagtg gcgcagagga aagcagacga
    36481 tggtcaggcc ggtttgcttt tcgcaaagta ttcctgacgg ctcacggcga catcggcaac
    36541 gaacatcacg cctgaatgcc gttcaaacag cggccgtagt cccgccaaaa taggctcgac
    36601 tttctcctga ggtaccacgg tcatgatcat ttcgaggctt tcctgttcgc tgaacatgaa
    36661 atgcgcctcg cgcacgccgt gatgaccctt gccggacaca tttcctataa tcgtatatcc
    36721 agtggcattg acgcggtcca gcaaatcagt cacaaaggca cgctgctcac cggccacgat
    36781 cacccgaatt tccttcattg gatgcaatat gaggcccatt cacggactcc tttcccacgc
    36841 ccactcaaac agtgtatgcc cactctcgca ctacaggcat cttacggaat aacgtgccat
    36901 actcccttcg ggtgatatcg ataccatatt ttatcctcag ggtctaaggc gacgagatgc
    36961 acccactcat tatggtaata gtgctggagt acttcatgcc gagagatcaa cttctcgatc
    37021 cgctcacgag gcgcttcaat cagggtgaga agccgcatgg gctcatggta tggcattgag
    37081 ccattcatga ccgtttgtct tgccagacct agtcgaaggt cgctccaggg gccggacata
    37141 atgccgagcc ggcccaccac attgtgataa attttgctcc cgctgccgta cacgtcattg
    37201 tcgaccgcag aaaaataatg ctccatgttg atccattggc cgacgacctg tggagcggtc
    37261 aatagcactt cgagcagtcg gttcgtcggg tcttcacggt agtcgtacga atggagaaag
    37321 acccggccat ccaagttcaa acctttggtc agttcccggc gcccgatgag aaatgcggtg
    37381 ttgccggaaa ggccccactc cggacgaact tggctccagt cggcactgcg acggcggacg
    37441 tgggtttggg cttgtctttc ctcgagggac tgcggcacgt cgggaaagcg cgcgcagcgt
    37501 tccaggcttg tcaacgccga ggcctccctg agatcctctt gcagccgggc caggtcggcg
    37561 cgatgcgtcg ggggcacgtc ctcgaggtcg aacagctgta cctcgtcggt cgtcgtgtcc
    37621 atttgcccgg ccaggaagtg cgtgtcattg gggatatcga tgccgccctt gctcaggcgt
    37681 tcccgcactt tgggattatt ggccatcatc gcgaggacgc gggcgttcgg cttgccttcg
    37741 ttccctccgc aggcgccgca gtcgagcgcc gattcgtatg gattgttatc cgaagtgctg
    37801 ccatgcgagc agaagagtac gagacgtgca aacgttttgg tcagacccat catgcggagg
    37861 gcggtatcaa cggtcagcgc ctgctcctca agggtgaagc cggttcgcgt gatccgttct
    37921 ttctggcggg atgccgatcg agcgtttaag tcgtattcgc ggcgcaggac ctcgacaaag
    37981 ccctgcaggg catccgccga aaggccggcc cgattcgccg cctcgatcac tgcctcgtcg
    38041 tgctcgtgtg agtcggtcag cgcccgctgg cgcaagccct ctaccagacc cggggtgatg
    38101 cgggatgccc gcagaccaag ccgttcttgg agcgcttttc ggacgagggc ctgctgatct
    38161 gcgaccagca tctcggcggt gtcttgcggg gccagcttgt cgacggtgag cgtggtggcg
    38221 atcgatggca caaacagccg ccttaaccac ccggtccaac gccggtacag cgaggggacc
    38281 aacgttttcc cgaagatggg aagagtgtag aaccatccca acgattccac catgacgtag
    38341 ggtgttacca cgttttcttt caaatcgtgc agcagcgtgt ggccggcgcg gacccacttg
    38401 gcgcgagcct tgtgtttcga cacccgatga tcgagatagc tccggggaat ttcgcgtact
    38461 tcattcttcg cccgcatgat gaccggaaat tgttcggtgt catgctcttt gccccaggcc
    38521 cgatagcgaa tgaacgcggc gaagaagccg gcgaagccgt aggtctcgtt gggccccgtc
    38581 gattcaagat gacggcgaaa cggttccgat cgcacgtcga tacagtagac tgattgcgaa
    38641 tacggacgag tcgcgttcgg gtgctcggct tcgcgagtcc tcgcccgcag cgctccgagc
    38701 agacgttcct gatagccggc ctcgaacgcg ttcagccaaa ccggaccgtg ctccgattcc
    38761 gggaaggcct cgagccattc gacgagctgt tgtagagttt ccggatccgt ctcaacgagc
    38821 tgttccggat cgatctccag gcaacggccc aacgccaaaa gttttcgcgc cgcccctcga
    38881 cgagtcgcgg cctcctggcg cggtaccact tcagccttgt accgatcgag taccgtcttc
    38941 cagccggcgc ctttctgatg agccagccgg tccacttcct ccgcataaag ggccggcaaa
    39001 cgaccggcga ctcgctggcg gcgcaaataa tattcctcgg cgtggctgcg catgtaggcc
    39061 accaccgcat cgtaccgtcc ttcgatgccg aggtggctgc tacaggcctt gtgcacgagt
    39121 tcccgggcgt accacagacg aatggcgagg tatttgacga gcccaacggg ataggcctgc
    39181 tgccaggggt aatcgcgttc ctcttcacgc catttaatga acccggccca gcccggcagt
    39241 gccgtcagtt gcagcgaaag gtagtcctgt ctgagccttg caggaattcc aagtgcgtct
    39301 aaatgttcga gcagcgcgtc ctctgggtac tctgggagct gcgcgatctt tcgtttgctg
    39361 tccgtgatgc cgcagggcga ccattcgtga gaggcaatgt ccttccaggc tttatagaag
    39421 cccttttcgc gttccggcat ggcccatgtg gcatgaccct cgtcgaggaa tgcctcgcac
    39481 catttgatca tctggtcgtt gatctgcccg acaatgtggg tgccgagcgt gtcatcgcac
    39541 cagtgcgaga gcgtgagcca ccggcccaag gcggcttcgt cgctgtccac gatgccccgg
    39601 atccgctctg ccaaatccgg aacggtcaag acggactcga gccgtcccgc cagtcgctcg
    39661 accacgccga gtgagtcatc gggcagctga ttgtccagcg gttcgacaac ggctgcacac
    39721 aggccctccg tcaggcagcc gcgcagcacc tctccgtgcg tcacaatccg ggaacccacg
    39781 acgacctctt tgccctgcac gcgcgaactc aatgcttcat cgaggtgccg gagctggatc
    39841 cgacctgaac gaagatactg tcggtacatg cggcccggga ggtagccatt cccacccatg
    39901 aattgcttgc cgcgacgaac cgtttcctcg aacggaagat attcgagact gtggagcgga
    39961 ttgtggtgca cgaacgtccg catcggccaa tattgggcga tgacttcgcc ggcgagcttc
    40021 acgatgccgc gcagctccat ccgtctggtt tggatgtcga tcgtacggtc tacctgttcc
    40081 acgtcgacga ttccttcaca gaaactgtct cagagccggt atgaccaggt gcaaagagcg
    40141 ttgccggggc caggcccagc gccaggagga ccaggacggc catcacgagt gatgccagct
    40201 ccgtcttgag cacatccgta tagcgcagct cctgccgtcg agtgccgtac aagagacgct
    40261 gaaccatctg catgatgtac caggatgcgg caagccaggc gagcagtgtg atgaacaggc
    40321 cgacggtaga gggaaagggc gcgttcaaca gcagccccat aaatccagca aagacgccga
    40381 agggcggcag gcccatggcc gccaacgcca gcaaggagag cagcaccgcg tattgcggca
    40441 taccggatgc caggccgctg acggcctgtg gatcgatgtc atctccatac ctggtccgga
    40501 cgacctgcca tgcgatcaag aggccgctcg tcgcgaggcc gaccgtgccc gccatgaggg
    40561 ccgctcgggg agttgccgtt cgcgtcgccg ccacgaacca ccacagcatc gaaaagaacg
    40621 aaaggctgcc ataggcgagg agcagccgta cgcgggactg cgccagcgcc ttgacggcgc
    40681 cgtagagggc ccccagtaag gcgaagaaac ttacggtcca ggcgaccacg tccggaacgg
    40741 tcggtagaac gtgtggaagc tcgtgcagac ccgcaattgg aagcagcaca acaatgaacg
    40801 ccggcaggtt gcccggcaac cgtgtcaagg ccgtgaggtg gccgtcgtga aatggcacca
    40861 gaggcaacag aaccgcgcac gtcaccagag aggcaatgga agaaaccggt ggaccagcga
    40921 cgaccgccaa cagagcgcac aaggcgccga ggccgtaggt cccgatgccc caccaagaca
    40981 tcggccatag ggcgctgtgg tggcggtaaa gaagaatagc aaccaggacc agcagcaacg
    41041 ccaacgcgag gttgccgacc agcggaacgc tcgtgagcac ggtcagtccc aatccaagac
    41101 agactaaggt catgatgcag gattggcgat acgcatcatg agccggctgt gcgagcaggg
    41161 aaatgatagc cgccatcggc aacaggtaaa gcggcaggaa cccttccggc ggcatcgcga
    41221 ggtatccggt gaagccgatg attgatgcac agctcacggc ggaccacaga atagaaaaaa
    41281 attttacgcg gtcggcattc ggccagaaga gaaggctgcc gactgctccc aataagggaa
    41341 tcccagtcaa tagccacgag aggagagcgc cgtcgatcat cgtgaccaac ccccttctcg
    41401 cttatcgaat tggtggaccc accgcatgag actatccccc agcagctggt ggagttcgtc
    41461 ggcatacagg cgattcatga agggcaagta caatcgtatt ctcaggttgt cgatccaccc
    41521 gggcatccgg atggtgcggc cgtgggcggt catgtacagg taggaccaac tggcgatcat
    41581 gagaatcgtg gcggcgagga tgatgaagtc gaacagccag ttgggaagag cggcggcctt
    41641 gaagtacgat gccacctcgt ccggattggg atacagaaac gccgtgaagg actcgacggc
    41701 gaatagatag acgaagacaa taaacaacag ggtcaccaac attgcagccg agaccttcca
    41761 ggaggcgacc tgccgcagtc tcgtcaacgt caagatggcc tgggacgagg taatccagat
    41821 gaagaacagg aagatgaccg tgccttgcga ttcgagcaac ggcatgcgca ggaccccgtg
    41881 cgtgagcaac agaatgaaga gtgggatcag cagggtggtg acgaatccgg tgacccaggt
    41941 cagacgggac aagccttctt cctctctttg atggtcgaca tgaggaaaat gcggctcctg
    42001 tctggccttg tgaatgacat tcccacaatt caagaacacg gtggccttga acaacccatg
    42061 cgcgatcaga tgaaacacgg ccagagagaa cgccccgagg ccacactcca tgatcatata
    42121 gcccatctga ccgatcgtgg agaatcccag cgttttttta atatcgttct gggccagcat
    42181 catcgtcgcg ccaagcacgg cggtcagggt cccgatcacg aaggcgacat gcagcgtcgt
    42241 cgaactcagg gcgaagagcg gagccagccg gttgatcaag aaccctccgg cgttgatgat
    42301 gccggcgtgc agcagcgcgt gtacgggcgt cggggcgaag agcgaccccg gcagccagaa
    42361 atggatcggg aactgcgctg actttcccat ggcccccacg aagaacagaa atgtcacggc
    42421 tgtggcccca ctcacctcca ttccgaaccc gagcggcaag ccgattggtg tttcggctgc
    42481 tcgaaggaac aggtcctgga attcgagcgt gccgtagagc tgataggcga gcgcgattcc
    42541 ggcgagaaat gccgtgtccg ccacgcgaag cacggtaaag gtcttgaacg ctccggtcag
    42601 ggtcccagca tgagcatgat tgtgcgcgag cacatagagg aggtagctga gaacctgcca
    42661 gaacagaaac agcatcatga gattggagct ggcgaccatg caaatgagaa caaaatctgt
    42721 cagacagatc atcgccagat agcggcgggc atgggcgtcc tgatacatgt acccgatcga
    42781 gtaggtataa atgatgacac tgacgccggt gatcagggtc atcatgaccg cgctcaagcg
    42841 atcaatataa aagccgatgg gaatggcaaa ggtcccgact gaagagagat cgtaaaactg
    42901 cagggcgatg gggcccccgg tcgtgacctg aagcagcgtc gcgaacgcgg ctaaaaaggc
    42961 gacgccaatg ggataggcgg ccatcttggc gcgtgcatgt cgggaactgt ccgagccgat
    43021 cagcaccagg ccggtggcaa ccagcaggag aagggggacc aacagcgtca acgtcatgaa
    43081 gacctcattc cctcgtgcaa cggtccatgc gtcgctcccc cgggaacgta cgaacgtctg
    43141 agggcccccc aaggcggcgg gctaccatta tacggtgtct tgacgttcgt caagcagggc
    43201 atgcatcaca cccgttcctc cgcgtgcttc tcaagccgtt gaaggagaga ccggcgggcc
    43261 tgctccaacc gctctgcccc ttcatctata tagaggcggt tcaagaacag cacatagagc
    43321 ttgatccgga tactctcgat ccaggtcggc aatggcagcg accggccgtg agccttcata
    43381 tagacatagc accagccgat gacggtgacg agcgttgccg ccccgatcat catgtcgaac
    43441 agccggccag gcagttccgc cgccttaaag taaagggcga cctcctgggg atccggatat
    43501 aagaaggcgg tgaacgactc gaccgcgaag aggtacacga agacaacgaa caagagcgtc
    43561 agcagcatcg cggccgatac tttccacgaa gccacggcgt gcaagcgggt cagcgtgagg
    43621 atggcctgag acgaggtgac ccaaatgaaa aagaggaaaa tcacagtgcc ttgcgcttcc
    43681 agcagaggaa tgtgcaggac gccgtgggtc agcaagagaa tcagcagcgg aatcaccaga
    43741 gtcgtaaaaa aaccggtgaa ccatgtcagg ggcgagtaac catgttcgtc cgtgtcatgg
    43801 ttcgcgtgag gaaaatgtgg ttcctgccgc gctctgccga tgacattgcc gctgttcaag
    43861 aacacggtgg ccttgaataa tccgtgcgca atcaaatgga agacggccag agagaacgcc
    43921 ccgaggccgc actccatcac catgtagccc atctggccga tcgtcgaaaa cccgagcatg
    43981 ttcttgatgt cgttctgcgc cagcatcatg gaggctccga gtaccgccgt cagcgtgccg
    44041 atgagaaatg cgatgtgcag ggttgtggaa ctcaaaccga acaagggcgc aagccggttg
    44101 atgaggaagc ctccggcgtt aatgatgccc gcgtgcaaca gcgccgtcac gggcgttggt
    44161 gcatacagat accggggaag ccagatgtgg aagggaaatt gagcggactt gctcatgccg
    44221 ccgatgaaaa gaagcaagga gacggccgtg gcgccgttca gttcaatgcc cggcagaagc
    44281 gagacggttg ccgtggattg agccgcagcg gcaaagatgt ccggaaattc caacgtgcca
    44341 tacagggagt aggccaaggc gatagccgcg agaaacgcga cgtcgccaac ccgcaagatg
    44401 gtgaacgtgc gaaaggcgcc gtccagcgtc ccggcatggg catggttgtg gatcaggaca
    44461 tagagcaaat atccgaggat ctgccagaag acgaacagca tcatgagatt tccgcttgag
    44521 accatgcaaa ggagcacgca gatcgcaaca ccgagcatgg cgaaataccg gcgctcgtga
    44581 ggctcctgat acatgtagtc gatggagtag gtgtaaatga tggtcccgat ggccgagatc
    44641 aacaccatca tgacggcact cagacgatcg atatggaaac cgatcgggac gggaagcgcg
    44701 ctgccttcag ccggttcgta gaatcggatt gaaatcggcc cgctggtcgc gacgacgtag
    44761 agagtgcaga gggctcccaa taaggcgacg gcgagcggcc aggcgccgat tttcactcgc
    44821 aggcgcctgg tcgactcgtt gccggcgatg acgaggagca ctgtcaacag gggacacagc
    44881 gggacaagca gagcggcggt catcggcgct tcatgactcc gtgaaacaaa agaaaagcca
    44941 accgatccct cgacaaaacg tcgatgccat tcggttggct tgtactgcga ccggcctttc
    45001 tgcctgacag ccggccaccc tacggcctag cggagtaatc ggcgtcctgt ccgtccatca
    45061 cgaactttga acgagcagca gcgccgtctc accataaata atatgacggg gcgaacgccg
    45121 tcaacagagc agtcggactc agggctcaat accaccgccc gatagcacta aaggcccaca
    45181 gcagacgggg atggtgagac aaagggctca ggatctactt gttcatgatt ccgtgcaact
    45241 ctataattcc gcaggctcaa ccgtggaaac cgcgatccgc aacgccatca tcaagttcga
    45301 gcaggagttc atgggccggg gcccggacga cgtccgggcc agtatcgtcc gtgatttggt
    45361 ggtcgtacgc ctcaaggggg tcctgacgcc ggcggaacgg cagttggcaa aaactgcgga
    45421 aggtgtcgac atggtgaagc gtctgcggca gaatttaatt gcgcaaggac gcgacaagct
    45481 ctgcgagcag gtgagcgaga tcaccggtgc caaggtgttg ggggtcttca cggacatcga
    45541 tgtgcaactc ggtgaacggg tctttgtctt cacgatggac cgggagctac agaacggcgg
    45601 acgttgacag cggcatgctt gagcgtgcgg tgatgggaga gcggagcaga tagacgatgg
    45661 aaaaactcgt taaaggcttt ttgaaattcc acggcgaggt cttcggaagg aagaaggacc
    45721 tctttatccg cctttcgcaa aaacaggaac cccgcgccct gtttatcacc tgttcggatt
    45781 cccgtgtcga tcctaccctg atgactcagg ccgagccggg ggaactcttc atattacgga
    45841 acgccggcaa catggttccg ccctacggat cgatgcaagg aggaagcacc gcgacgatcg
    45901 agtatgcgat ggccgtactc aaagtgccac acatcatcgt gtgcggtcat accgattgcg
    45961 ccgtcatgaa agcgttgttg cacccggaag aggtccagga gttgcccgcc gtgagggaat
    46021 gggtcgggca ggcggagacc acgcgacgct tgatgcggga gcactatacc gacctcgaag
    46081 gcaatgcccg tttgatcaaa acgacgcagg aaaacgtccg cgcccagtta gatcatctca
    46141 gaacgcatcc ctccgtcgcc ttgcagctcc gcagagggaa ggtcgatctc cacggttggg
    46201 tgtactccat tctgaccggt gacctttggg tctacgactt cgagagcgag caatttacgc
    46261 cgctcgtgga cgaggcgccc ccgcgcttga aagccgcccg gtagaaccgt ccgacagcat
    46321 cgttctcgca tcgcgccttc cgaccatgcc caaggctctc cagttgtaaa ctgcccctca
    46381 gccctggcat gctgggtcga tgggcgatcg tcaactcgaa accctcacgt ttcacaacac
    46441 gtatgcccgt cttccagagg cgttttacgc caaactgaat cccaccccct tctcgtcgtc
    46501 accttatctg gtcagtttca acgctgccgc cgccgatctc atcgatctcg atcgtcgaga
    46561 agcggagcgg ccggagttca ccgcgctctt cggtggcagc atgttgacgc cggcgatgga
    46621 gccgcttgcc atgctgtatt cgggtcatca attcggcgtc tacgtgccgc agcttggcga
    46681 cggtcgcgcc atccttctgg gtgaagccac caatgcacgg ggcgagcgat gggatctgca
    46741 tctcaaaggg gcaggcctga cgcccttctc ccgggacggc gacggccgtg cagtgctgcg
    46801 ttcgacgata cgggagtacc tatgcagcga ggcgatgcat gcgctcggca ttcccacgac
    46861 gagagcgctc tccattatcg gcagcgacga gaaggtgtac cgggaacagg tggagagcgg
    46921 cgcgatgctc ttgcgtatgg caccctcaca tgtccgattc ggcaccttcg agatcttcta
    46981 ttatcggcgc caacacgaac atctcagagt cttggccgac tacgtcatcg actatcatta
    47041 ccctcacctc cgagagaccc cggacaaata tgcggcgttg tttgcccaag tcgtggagcg
    47101 cacggccagg ttgattgctc ggtggcaagc agttggctgg gcgcatggcg tgatgaacac
    47161 cgacaacatg tctatccttg gcctgaccct cgattatggt cccttcggct tcatggatga
    47221 ctatgatgcg ggattcatct gcaatcactc cgatcacaac ggccgctatg ccttcaacca
    47281 gcagccgttt atcggattgt ggaacctgag ctgtcttgct caagccttgt tgccgctcgc
    47341 ggagaaggac gccttgaagg agggactcga tggctatatg ccgctcttcg agcgggagta
    47401 ccgatcactc atgcgggcca agcttgggct ggcggatgca cgagacgaag acgaggaact
    47461 catccgtgaa ttcctgggtc tgctccaagg cagccatgcg gactatacca tcgtgtggcg
    47521 agagttgggc gggtttctct ccggacgagg aatggtcaat gacaggttgc gcgaacattt
    47581 tctcaacaga gaacgcttcg acgcatgggc cgttcagtat gccgagcggt tgcgagccga
    47641 gcgcagtcgg gatgacgaca ggcaacaagc catgaaccgt gtgaatccga aatatgtgct
    47701 ccgcaattac ctggcccaag cagccattga aaaggctcag caaaaggact tcgctgagat
    47761 cgaccgtctc ctcgccttgc tgcaacaccc ctatactgac cagcccggaa tggagggata
    47821 tgccgcccct ccgcccaatt ggggcaaaca cctcagcgtg agctgctctt cctaaatacg
    47881 gcccttcctc cgttctgtca gttgtgcata cattccacgg tcgagtgcct tgttgcagtc
    47941 cccctcattg tctggtgtac agtggtccga tttttgcgcg aatgaggaga agccatccgt
    48001 ggccaagcct cgaggagatt caaaattagc ggtcgcaggc gcactcacgc tggtactggc
    48061 cattgccggc gtgttgttgg tcaaagagcc cctgcgcagc tcacgacccg tgggtagcgg
    48121 actggagatg aagcagtcca ccgccgagca gttagtcagg gcgcgcttgt gggaagaccc
    48181 tgtggctgcc gttgaccggg cgattcgtga gcaaggagcc accaagacca catccagagg
    48241 atcgtctttg gccaaacgcc tgagcccact tcgtcatgcg attgcccagc gggtccatcg
    48301 tggagagcaa ctcacggttc tgctcatcac gactacgggg ggcggctatg tcgagagtac
    48361 ggaatcgcgc cttcgcgatc ggtatgccgt cggcacggct cttggagccg cctgctatgc
    48421 gccggaagac gagggtcatc tgtccttcgt gcattgggaa ccgggcagca cagttcaggg
    48481 gctgccctat gagtggtatc gtcttcgaaa gacgcgcgtc tgcggggccg caggctcacg
    48541 cgccgacagc atccttatcg tatggttacc cgacgaagcg gtcacggccg gctttttcgc
    48601 caccttaaca tcgttgtcgc aagggctcgt atgtcgtgaa gacagcaaca acgagtgtct
    48661 gctcaacggt gcgaaacctt ctctcgtacg cctggatccg accctacagc aagccgcctc
    48721 tttcaagatc gtcggtccgc gcagctcatc ggcctatcgg gcgcttctcg atgaagccgg
    48781 tgcattgacc ggcaacgcgc agcaaggcgt tgggatctgg ccgaatgcgg acggttggat
    48841 cgagctgtac tcgccttggg ccagtgcgat gaaaggtctt ttggcctatg gtctcaagtc
    48901 ggagtcaggc tcgtcctgta caagctacga agaatgtgag caccggttct atcgccggct
    48961 atcggacgcc aaggtccgag tcctctacga cgtcgggtca gacgagatac gctttcaatc
    49021 gttgatcgcc gaattggaac gacggcaggt tcgcctgggc tgggacgccg tgattcttat
    49081 cggtgaatgg gactcgtttt atgggcgcac ccttccgatt gaatttcgcg ctgcggcctg
    49141 cgcgaaaatt gcgacgttcc cggaaacgga tctcaaggct attcaggtgc cggttcaggt
    49201 caaacaatgg tgccagacga ttgcccaggc gatcgatctg cagatccaac gtcctgccga
    49261 ctacgagtcg ctgctgctca acgtcttccg atacagttac ctgagtggat tggacggcga
    49321 aatcccaggt gacgatgcag ccagagcggc acgcgccgcc aaggccggcg ccgacgcaaa
    49381 aggaaaagac gcgacgcggg accggccgga aggcacgggg caaaccgact atgtgcgcgc
    49441 gctggtggcg cgcattcacg atgaagggga aggggcccgt gcgatcggca ttctgggcac
    49501 agatccctat gacgcgctgc tgatcatcaa ggcgctgcgc cccgcgtttc ctcatgcgat
    49561 cttttttacc gtagatctcg acgcgcgcca cttgcatccc agcgaataca agtccacgcg
    49621 caacatggtc atcgctgccc ccttcggatt gcagctggag ggcggtcttc agcgggacgt
    49681 tccgcccttc cgcagcagtt accaaacttc agcgtacttc gccgtgttgc aagccgtcgg
    49741 tcacgtgcgc tgtgagcaac agggccatca acagcaaaca gggccctgca cgaccagcta
    49801 ccatgtcgcg cagactccgg atgatcgcct ctatgacgct gcctcccatc cacgactctt
    49861 tgaagtcggg agagagggcg ccgttgactt gagcatggtt gaaaaggaag gcgtgcgcac
    49921 catccatccg ttgcgggcgg atctcgacta tacggacgaa catggacaac tgaagcaggg
    49981 cattgggttc gatgacagcg ccgttgccgc actcctcgcc gtcggggtgg tgctcggcac
    50041 catcatcgtg tggagcaatc agcggctgtg gctgtgggtg tcgcgaaatc ccgtatggat
    50101 tggactgtcg gccgtcgtgc tcctggcggc gttctgggcg tttgtattca ccggcggtgg
    50161 ctccatgctg cttgccgggc atgacgaggg agaaccgttt tcctggacgg ccggcgtcag
    50221 tatctggccc agtgaactga ttcggctttt cgtcgtcgtg ctctcgctcg cgctgctcat
    50281 caaaggctcc agggatctga tgaggaacag tgacctcatc agccgcgatt tcggactcgc
    50341 gcggcacacc gcgcacggaa cattttcgct caagaccttc tggatgaatc tgaagcgggt
    50401 ctcccatccg actgagacga tgaccccgac aaccttggac caagcctggc agtggtactc
    50461 cgaagcgagc cggccggccc agtggctggc tcgaacggtt tttctctttc ttctctatct
    50521 cggcatcatg tggtcattga gcgcttatgt ggtggacgag gagtacatcc atccctgtcg
    50581 cggccggctc agttgcacga tcgattcgtt catgacgctg agtagcgccg cgctcgtggt
    50641 cttcctcaac ctggcggtct tcgatgccgt gatgttgtgc cgccggtgga tcgggtgggt
    50701 gacggcgtcg accggtggct ggtccgagca ggtccaggag aagtatctca gggaatacgg
    50761 gttgcgtccg gatcagaaag tggaattcga aaagttgaaa tacctggccg cagtggattt
    50821 gatcgggcgg aggacggaag tggtaaaccg gctgatccgc tatcccttca ttgctctcct
    50881 gatcatgatg gcagcccgta acgactactt cgacatctgg aactatccgc tgctactcct
    50941 gttttcctgg gcggtgaatg tcgtgctggc gctgatggcg gcgtttctgc tgtatcaggc
    51001 ggcaagtcag gccaaagcgg cgatgctcgc cggactgagc cgacagatgg tgcaggcatt
    51061 gg
//
LOCUS       DASSCQ010000044        13644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1740678, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000044 DASSCQ010000000
VERSION     DASSCQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13644
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740678"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2018
                     /gene="asnB"
                     /locus_tag="VFH05_03350"
     CDS             <1..2018
                     /gene="asnB"
                     /locus_tag="VFH05_03350"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6677276.1"
                     /translation="RLVHRGAGLGRDVSSSRNKILRTGGRLTMCAIAGILKFDHRERA
                     ERSRLERMSTVQQHRGPDGSGTWLDGPVGLAHRRLAIIDVDGGHQPMTNEDDSLWIVF
                     NGEIYNHAVLRREMERAGHRYRTRSDTETILHLYETYGPRCVDRLQGMFAFAVWDRRE
                     RRLLLARDRLGIKPLYYASNDHELLFASEIKGIMASGVPDASFNQAILPEFLANRYVA
                     GAETFYRGVLKLLPGHLLEWSAEQGTTIRRYWHPPQLSVDDRTPLAQQADQLRAGLQT
                     AVQSHLMSDVPLGLFLSGGLDSSGLAALMAPMMPQGLKTFSVGFAEGEANELQYARLV
                     ARAVGSDHHEVLVSPEQFMQALPKLVWHEDEPIAFASSIPLYFVSRLARQHVKVVLTG
                     EGADELFLGYDYRYRVTALNDRIGRFYWSSVPRALRRRISGSLGRLPLRIQRYALRTA
                     LAYEPGPRGLFFDNFSVFPESLQRLLLKERSATGPDPYAEGLRYWHEHGGTLLERMSY
                     ADVGTHLVELLMKQDQMSMAASLESRVPYLDHLLVEQVMRIPGDQRMRRWQTKVILRE
                     ALRKMVPPAVMNRSKMGFPVPFGSWVRGPFRTILDEYVLSTRALDRQLFDPGTVRRIV
                     DEHVSGIRPHTDRLWLLVNLEMWQRIAIDGEMPQPLEEQVEAETVPA"
     gene            2083..3240
                     /locus_tag="VFH05_03355"
     CDS             2083..3240
                     /locus_tag="VFH05_03355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6677277.1"
                     /translation="MIEGQSIICISQDWEGDPTSKKHIMRILSARNRVLWVNSIGMRR
                     PTTAASDLRRLVAKLRRIVSGVRLVEPNIYVMDPPVLPLPGIRAAEQVNRIVLAARLR
                     QLCRRLQMQRPIIWTFLPNVNWLLGRLHERMVIYHCVDEYSQFSGVPKDVIIRMEREL
                     IQRADIVFTSAEKLCAERREINPRTYFIPHGVDVAHFSRALDPDTAVASEIRTLPKPV
                     IGFFGLLADWVDLDMIGALARARPKWSFALVGKAQTDLQAVQGLPNVHLIGQRPYSAL
                     PGYCRGFDVGLIPFRMNELTLRVNPLKLREYLAAGLPVVSTPLPEVVRYQGVVHIATN
                     REGFLEEIEAALKERTSERDRARVAMMQSEGWEARVGEMSRLVEERLTEAA"
     gene            3237..4922
                     /locus_tag="VFH05_03360"
     CDS             3237..4922
                     /locus_tag="VFH05_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677278.1"
                     /translation="MSYRSSWMCPLMVAIVMLAIPTGEGCAATASGVRPALPHEYVDT
                     TVKSSPGRTLTVPAGGDFQAMLDEAQPGDEIVLTAGARYKGPFTLPYKKAGSGWITIR
                     SGGIGKDFPAPGTRVQPSQAGRMAALVSNKHGVILAEPRAHHYRFIGIEIKPEDNTLL
                     RDLVLLGDNTARSLDDLPHHIILDRCYVHGDPKKGSRRGVVMNGRHLAVVDSHVSGFR
                     EEGQDSQAVVGWGGSGPFKVENNFLEGAGENLLFGGGDPSIRDLVPSDIEIRHNHLVK
                     PLSWKPGEPEHDGGNWQVKILFELKNARRVLVDGNIFEYNWQRPGYGFAITFTVRNEE
                     GRSPWAVIEDVTFTNNIVRHSPSGVNIMGSDDIHPPSGQGQRILIKNNIFDDINGPRW
                     GGKGRLFQLLRGTKHVVIEHNVGFHTGHTVMAAGDPHEGFVYRYNITPHNEYGMEGEG
                     TGVGRPTLERHFPGAIVTHNILIGGESYAERYPEENTFPASVEAVPFMNAAAGDYRMA
                     ASSAVSGSPSHEVPGIDAKSLCAALGDTGRMEAVCSKPLSKDRGHNPHAAKNE"
     gene            4937..5731
                     /locus_tag="VFH05_03365"
     CDS             4937..5731
                     /locus_tag="VFH05_03365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6677279.1"
                     /translation="MEHQTALQEWEQAEIRRSAAEAESADASTLIASEDNVARYVNPP
                     ADTLFHLEYAFHLLGDVTGQQVLEYGSGWGENTIILARLGAEVHALDISPELIAINRR
                     RMQVNGVTSGVRFSVASAYEVPCPDGSVDVVFGMAILHHLDLDRAAREVYRVLRPGGR
                     AIFSEPMRNSPALRALRKMVPCPLPANVSPYERPLTLGELAKFSTGFVAGRSRSFELP
                     WSRAAERIPALRTSLMPLRRWDRAMLARFPGLAYYAAIRVFEIRKP"
     gene            5794..7026
                     /locus_tag="VFH05_03370"
     CDS             5794..7026
                     /locus_tag="VFH05_03370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6677280.1"
                     /translation="MTTIVLTWSAMVLLVYTYAGYPVLLWLIGRLKDPLTTERGTTNS
                     WPSLTVVISAYNEEAVIARRIQNLLDQDYPSEQVEILIGSDGSTDATCQVVESYRSAG
                     VQLAAFRTRRGKASVLNDLVRLAGGEMVIFTDAATVFYPDAFKQLVTGFWRYPSASVV
                     VGELEMRSSRGSRNLDGWYWRYELFLKGRESEIGAGLGASGTIYAVRRQDYCPLPPDT
                     IADDLLEPLLVRLRTHGDVVQYGAARAWQLIPERVADEFHRRVRSGAGIAHVLRHAWP
                     LLLPQRGLVSLALWSHKALRLVAPWMLLTVLAGTLFLRHSPFSQWLLISQALFYGAAL
                     CAGWLRAVPLIGKAAIGARYFVVLNAALALGSVKFFLGLSRPTWNRTRRPEEEAMRAD
                     DWSEVMPDDAVEKQRPAV"
     gene            complement(7046..8446)
                     /locus_tag="VFH05_03375"
     CDS             complement(7046..8446)
                     /locus_tag="VFH05_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677281.1"
                     /translation="MLFVTKSAAILRAAVCCSLVLLVATAVVGQTTTSFPGGWIEKQG
                     TYTRARYTASQIQSFVPPARGTFTFPAPYRTKGIRITDASDCGGADCVNYIGYSYWRN
                     TNAHESSADMWIFVGLSTARGGQGPTLFKLNKATDVITKVGPLFPAGSPFRSQSGETW
                     YFSASRPNKLYINDGPRLLRYDVVSKVSDTVFDITAQFGSGRYVWQTHSSNNDMVHSG
                     ALRVSATGEDLGCFVFNETTRKFTFFPKVGTYDECHLDKSGKYLMIQENRDGQYGLEN
                     VFIDLETGKQSVVMDQNGGLGHADMGFGYALGMDNWNSLPGAAITFNLAGIVKGPAVF
                     YSNNWNMNPVNHVSHLNAKSTIPMSQQFACGSNADYGSVQNEVVCFRLDASHDQLIVA
                     PVMTDMNAPGGGSEYAKYPKGNLDISGRYFIWTSNMGGNRLDAFLVKVPSHLLTGSGD
                     YAAPAAPVNLRVTTSR"
     gene            8666..9541
                     /locus_tag="VFH05_03380"
     CDS             8666..9541
                     /locus_tag="VFH05_03380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6677282.1"
                     /translation="MEIAERSRQDFAEEHVEEVRQGRRFEFGDNWYRFLQNLKEEQIV
                     SAETSLREMLEISDLTGKKFLDAGSGSGLFSLAARRLGAQVHSFDYDPQSVACTKELR
                     RRYSQNDPWWRIEHQSVLDQDYLSSLGRFDVVYSWGVLHHTGAMWKALDNVHRSVASG
                     GLLFIAIYNDTGTQSARWRRIKQLYNALPRPLRWPYTAVVAAPLEFKAVLSALASGHP
                     SSYIRRWTDKNERGMTHWRDAVDWIGGYPYEVAKPEQIFEFYKARGFSLRRLKSTVGL
                     GCNEFVFRKDREVKA"
     gene            complement(9557..10249)
                     /locus_tag="VFH05_03385"
     CDS             complement(9557..10249)
                     /locus_tag="VFH05_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677283.1"
                     /translation="MAKILVVEDMDSVVDLLRSLLHREGFEVAVAQDGPEALEAVRRE
                     KPDLILLDLILPGLDGLEVLRRIRQDPITAHLPVIVLSGKDEERDKVIGLEIGADDYI
                     TKPFQANELIARVKGRLRRSAPTDPARVLKVGSLEMELDRYQVTLRGKPIHLTSKEFS
                     LLRALLMTNGRVLSRDALFESVWGASKDADLQSRTIDVHIRSLRNKLGAEGRRIFTVR
                     NVGYRLDPAPTE"
     gene            complement(10268..10960)
                     /locus_tag="VFH05_03390"
     CDS             complement(10268..10960)
                     /locus_tag="VFH05_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677284.1"
                     /translation="MPSPRTSRLENRNAEPAPSLVSCRLLLADDHTLLRQGVKALIET
                     YGVTVVGEAGDGREAVRMAEEMQPDVAVIDVMMPLLNGLDVVREIRRVSPGTKSLLLT
                     MHTDDAFVLEALKVGVKGYLLKSQPVTDVVHAIRDVSKGSFYLSPGISQTVIQAFLAK
                     SDIPSDPLTTREREVLQLVAQGMSTKKVAGMLGVSVKTAESHRHRIMDKLDLHDTASL
                     VRYSIRRRLIEP"
     gene            complement(11508..13007)
                     /locus_tag="VFH05_03395"
     CDS             complement(11508..13007)
                     /locus_tag="VFH05_03395"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HET6677285.1"
                     /translation="MPISPAPLPSTFWVRLSLSAIAAICLITQLQCGGSGDSNGGGSS
                     PPPGVTGDVKAIARENTLPGTTAWRIQNPENGGEIAAYPDRESYPGGAEVRISVSANP
                     PGNFRWQVFRMGSYGGMGGRLYAEGGPHAAPRQADLTFDPVTGLMVAGWPATFSFPTR
                     RSDGTPWLTGVYLVMLTKDDGRQTAALFIVRDDTRDAEVGVQLPTATYQAYNNYGGES
                     LYESSHGLSGGKARKVSLDRPIARQYGNGTGTFLYQEHEAVIWLEDHGYDVEYQASSD
                     TGGANGRIGAHRLFTIIGHEEYATMAEFDRLQSAVANGTSLAFLTGNTLYWQIRYENN
                     DRVIVGYKDHAAEDPMRLTNPSLVTSTFRNPPVNRPENQLVGVMSDGSFITQPSDWVV
                     TNANHWVYAGTGLSNGSRIPNLVFTEWDALVNNGLTPSGLTVLASSVVPNNIPAIPSR
                     HESTIYERGSAFVFAAGSIFFSQHIRSQPAVGQMVANLLARAGAGAHEP"
     gene            complement(13237..>13644)
                     /locus_tag="VFH05_03400"
     CDS             complement(13237..>13644)
                     /locus_tag="VFH05_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6677286.1"
                     /translation="LSVPASVGFINLFGVAVLNGIVLVSYINQLRAEGLPDHEAIVRG
                     CLLRLRPVLMTALVAMLGLIPLAFSHGIGSEVQRPLAVVVIGGLVSSTLLTLIVLPTL
                     YGVFAGSPKPVTADSRARQQFSLPGDRPAPSPH"
BASE COUNT         2775 a         3829 c         4132 g         2908 t
ORIGIN      
        1 tacgattggt ccaccgtggg gccggcctgg gccgagacgt atcgtcgtct cgcaacaaga
       61 ttctccgaac gggaggtcgc ctgacaatgt gcgccatagc aggaattctg aaattcgatc
      121 accgggagcg ggcagagcga tcccgtctgg aacgcatgtc gacggtgcag cagcaccgtg
      181 gtccggacgg atcggggacg tggctggacg gaccggtagg tttggcccat cgtcgcctgg
      241 ccatcatcga tgtcgacggc ggacatcagc cgatgaccaa cgaagacgac agtctgtgga
      301 tcgtcttcaa cggtgaaatc tacaaccacg ccgtgctccg tcgtgaaatg gaacgagcgg
      361 gccatcgtta caggacccgg agcgataccg agacgattct tcatctgtac gaaacctacg
      421 gaccccgttg cgtcgaccgg ttgcaaggta tgttcgcgtt tgctgtctgg gatcgccgcg
      481 agcgccggtt gctgctcgct cgcgatcggc tcgggatcaa gccgttgtat tacgcctcca
      541 acgatcacga gttgctcttt gcgtcggaga tcaagggcat catggcttcc ggcgtccctg
      601 acgcgtcgtt caaccaggcc atcctgccgg aatttctggc gaatcgttac gtggccggag
      661 ccgaaacatt ttatcgcggc gtcttgaaat tattgcccgg gcatctcctg gagtggtcgg
      721 cggagcaggg gacgacgata cgccgctatt ggcatccgcc gcagctcagc gtggacgaca
      781 gaacaccgct cgcccagcag gccgatcagt tgcgggctgg actgcagacg gctgtgcaga
      841 gccatctcat gagtgatgtg ccactgggtc tttttctttc cggtggtctg gattccagcg
      901 gccttgcggc gctgatggct ccgatgatgc cgcaaggatt gaaaacgttt tcggtcggtt
      961 ttgccgaggg agaggccaac gaacttcagt atgccaggct cgtcgccagg gccgttggtt
     1021 ctgatcatca cgaagttctc gtttctcccg aacagttcat gcaggcgttg cccaagcttg
     1081 tctggcacga ggacgagccc atcgcgtttg catccagcat cccgctgtat ttcgtatccc
     1141 gcctggcccg acaacacgtg aaggtggtgt tgaccggtga gggcgccgac gaactgtttc
     1201 tcgggtacga ctaccgctat cgtgtcaccg ccctgaacga tcgcatcggc cgattctatt
     1261 ggagcagcgt cccccgcgcg ctcaggcgac ggattagcgg ctcgttgggc cgtctcccct
     1321 tgcggataca gcgctacgcc ttgcgaaccg cgttggccta tgaacccggc ccgcgagggt
     1381 tgttcttcga taatttttcc gtgtttccag agtcgctgca gcggctgctg ctgaaggaga
     1441 ggtccgccac gggtcctgat ccctacgccg aaggtctgcg ctactggcac gaacatggcg
     1501 gcacgttgct tgagcggatg agttacgcgg acgttgggac gcacctcgtg gagctgctta
     1561 tgaagcagga ccaaatgagc atggcggcgt cgttggaaag ccgcgtgccg tatttggacc
     1621 acctgcttgt cgagcaggtc atgcgaatcc ccggcgacca gcggatgaga cggtggcaga
     1681 cgaaggtcat tcttcgtgag gcgctacgca agatggtgcc tccggcagtc atgaacagat
     1741 cgaaaatggg gtttccggtt cccttcggga gttgggtgcg cggccccttt cgaacgatcc
     1801 tcgacgagta cgtcctcagc acacgcgcct tagaccgtca gctctttgat cccgggacgg
     1861 ttcgccggat cgtcgacgag cacgtgtcag gcattcggcc tcatacggat cgtctctggc
     1921 tcttggtgaa cctggaaatg tggcagcgga tcgccatcga cggtgagatg cctcagccat
     1981 tggaagagca ggtggaagcc gaaacggtgc cggcatgacg tggaggaaaa ttctgaacct
     2041 ccgaaggtat ttctgcaggc gtgttgaaag gagtgaaccg tcatgattga agggcaatcg
     2101 attatttgca tctcccaaga ttgggaaggc gatcccacca gcaagaagca catcatgcgg
     2161 attctctcag cacggaaccg cgtgctgtgg gtgaactcga tcggcatgcg ccgccccacg
     2221 acggccgcaa gcgacctccg gcgattggtc gcgaagctgc ggcgcatcgt ctcgggcgtc
     2281 cgcttggtcg agccgaacat ctatgtcatg gaccctcccg tgttgccgct gccaggtata
     2341 agggcggcgg agcaggtgaa tcgcattgtg ctcgcggccc ggcttcgaca gctttgccgg
     2401 cgattacaga tgcaacggcc catcatttgg acatttctgc cgaacgtcaa ctggctcctg
     2461 ggtcgcctgc acgaacggat ggtgatctat cattgcgtcg atgagtactc tcaattttcc
     2521 ggcgtcccga aagatgtcat cattcgcatg gaacgggagc tcattcaacg cgccgacatt
     2581 gtgtttactt cggccgaaaa gttgtgtgct gagcgccggg agatcaatcc gaggacctat
     2641 tttattccgc atggggtcga tgtggcgcat tttagcaggg ccttggaccc cgacacggcc
     2701 gtggcatccg aaatcagaac tctcccgaag ccggtgatcg ggtttttcgg cttgttggcg
     2761 gattgggtgg atctggacat gatcggcgcg ctggccaggg cccgcccgaa atggtccttc
     2821 gcgctagtgg ggaaggcgca aaccgatctg caggcggtgc aagggttgcc caacgtgcat
     2881 ctcatcgggc aacgacccta ttcggcgctc ccaggctatt gccggggctt cgatgtcggc
     2941 ctgatcccgt ttcgcatgaa cgaactgacc ttgcgggtca atcctctcaa attgcgcgaa
     3001 tacctggcgg ccggccttcc ggtagtgtcc acgcccttgc ccgaagtcgt gcgttaccag
     3061 ggcgtcgtgc acattgccac caatcgagaa gggtttttgg aagaaatcga agccgcgctg
     3121 aaggaacgaa ccagcgagcg ggaccgcgcc cgagtggcga tgatgcagag cgaagggtgg
     3181 gaggcgcggg tcggcgagat gtcgcggctg gtcgaagagc ggctcacgga ggcggcatga
     3241 gttaccgatc ttcctggatg tgtcctttga tggtcgcgat cgtgatgctg gccataccca
     3301 ccggtgaggg atgcgccgcg acagcctccg gcgttcgccc ggcgttgccg cacgagtacg
     3361 tcgatacgac cgtgaaatct tcaccggggc gcacgctcac cgtcccagcc ggcggtgact
     3421 tccaggccat gctggatgag gcgcagcctg gtgacgaaat cgtcctcacg gctggcgccc
     3481 ggtacaaagg gccttttacc ttgccgtaca agaaagcggg ttctggctgg atcaccatcc
     3541 ggtctggggg gattggaaaa gactttccag cgccgggaac acgcgttcag ccctcgcaag
     3601 ccgggaggat ggctgcactg gtatccaata agcatggcgt catactcgcg gaaccacggg
     3661 cgcatcacta tcgcttcatc ggcattgaga tcaagcccga agacaacacg ctgcttcgcg
     3721 atctcgtgtt gctcggggac aataccgccc ggtctctcga cgacctgcct caccacatca
     3781 ttctcgatcg ctgctacgtg catggcgatc cgaagaaagg atcgcgccgc ggcgtcgtga
     3841 tgaacggcag gcacctggcc gtcgttgact cacatgtgtc gggctttaga gaagagggac
     3901 aggattctca agccgtcgtc gggtggggcg ggagcggtcc gttcaaggtt gagaacaact
     3961 tcctcgaagg agcgggtgaa aaccttcttt ttgggggcgg agatccctcg attcgtgatc
     4021 tcgtgccatc ggatatcgaa atccgtcata accacctggt caagccgcta tcatggaagc
     4081 cgggagagcc ggaacacgac ggcggcaatt ggcaggtcaa aatactattc gagttgaaga
     4141 acgcgcgccg cgtcctggtg gacggcaata tctttgaata taactggcag cggcccggct
     4201 atggattcgc gatcacgttt acggtccgta acgaggaagg tcgttctcct tgggcggtca
     4261 tcgaagacgt cacctttacc aacaacatcg ttcgtcatag tccgagtggc gttaacatta
     4321 tgggttcgga tgacattcat cctccgagtg gacaaggcca acgcatcctg atcaagaaca
     4381 atatttttga cgatatcaac ggtccgcgtt ggggcgggaa gggccgattg tttcaattat
     4441 tgagaggcac gaagcacgtc gtcattgaac acaatgtcgg attccacact ggtcataccg
     4501 tgatggccgc cggcgatccg catgaagggt tcgtttatcg gtacaacatc acgccgcaca
     4561 acgaatatgg catggaaggg gaggggacag gagtgggcag accgactctg gagcgccatt
     4621 ttcccggagc gatcgtgacc cacaatatac tgatcggcgg tgagtcctat gctgagaggt
     4681 atccggaaga gaacacattt cccgcctcag tggaggcggt gccgttcatg aatgccgcgg
     4741 ctggagatta ccgcatggca gccagcagcg ccgtgagcgg ctctcccagc cacgaagtcc
     4801 ctggaataga cgcgaaatcc ctgtgcgccg ccctcggcga taccgggcgg atggaggctg
     4861 tctgctcgaa acctctctcg aaggaccggg gccacaaccc tcacgctgct aagaacgaat
     4921 gaaccgaaag gatgttatgg aacatcagac ggcattacag gaatgggaac aggcggaaat
     4981 caggcgcagc gcggcagagg ccgaatcggc tgatgcctcc accttgatcg catccgaaga
     5041 caatgtggcc aggtatgtca atccgccagc cgacacgctc tttcacctgg aatatgcttt
     5101 tcatctgctc ggcgatgtca cgggtcagca ggtgttggag tacggctccg gctggggtga
     5161 gaacaccatt atattggctc gattaggcgc ggaggtccat gctctcgaca tttcgccgga
     5221 attgatagcg atcaaccgtc ggcgaatgca ggtcaatggt gtcacgagtg gcgtacgatt
     5281 cagtgtggca tcggcttatg aggttccatg tccggacggt tcagtggatg tcgttttcgg
     5341 catggcgatc ctccaccatt tggatctcga tcgggcggcg cgcgaggtgt accgggtgct
     5401 gcgaccgggg ggccgggcca tcttcagcga gccgatgcgg aactcgccgg cactgcgcgc
     5461 tttacggaag atggttccct gcccgctgcc cgccaacgtg tcgccgtatg agcgcccgtt
     5521 gacgcttggc gagctcgcaa agttctcgac gggattcgtg gcgggcagat cgagaagttt
     5581 cgagctgcca tggtcacggg cggctgaacg gattcccgcg cttcggacga gcctgatgcc
     5641 gctgcgccgg tgggaccgcg ccatgctggc ccgctttccg gggctggcct attacgcggc
     5701 gatccgggtg tttgaaatac gaaagcccta gtctctgtcc attccagagc tgtctggcat
     5761 acgaatcctt ccatttttac taaaggtata tcaatgacga cgatcgttct cacgtggtcg
     5821 gcaatggtct tgctggtcta cacctacgcg ggataccccg tcttgctctg gctgatcggt
     5881 cgactcaaag acccgctgac gacggagcgc ggcacgacga actcttggcc atcactcacg
     5941 gttgtgattt ccgcctacaa cgaggaggcg gtgatcgcac ggcgaatcca aaatctcctg
     6001 gaccaggact atccgagtga gcaggtagaa attctcatcg gctcggacgg gtcgacggat
     6061 gcgacctgtc aggttgtcga gagctaccgg tctgccggcg tgcaactggc agcttttcga
     6121 acccgccggg ggaaagccag tgtattgaac gatctagtca ggctggcggg tggcgaaatg
     6181 gtgattttca cggatgcagc cacggtgttt tatcccgacg cgttcaagca attggtcacc
     6241 ggcttttggc gctatccatc cgcatccgtc gtggtaggag aacttgaaat gcggtcgtct
     6301 cgcggctccc gcaacctgga cggatggtat tggcggtacg aactcttctt gaaagggcgt
     6361 gagtcggaaa tcggcgccgg tctcggggcg agcggcacga tctacgccgt tcggcgacag
     6421 gactattgtc cgctccctcc ggacaccata gcagacgatc tgttggaacc gttgctcgtc
     6481 cggcttcgca cgcacggcga tgtcgtacag tatggggccg ctcgcgcctg gcagctcatt
     6541 ccagagcgag ttgcggacga atttcatcgg cgcgtgcgat ccggcgctgg aattgcccac
     6601 gtgctccgtc acgcgtggcc attgctgttg ccccagcggg ggctggtgtc actggctctg
     6661 tggtcgcaca aagccctgcg gctcgtggcc ccctggatgc tgttgacggt cctggccgga
     6721 accctctttc tgcggcattc tccgttctct caatggctgt tgatctccca ggcgcttttc
     6781 tatggggcag ccctgtgcgc cggatggtta cgtgcggtgc cgctcatcgg caaagcggcc
     6841 attggagcgc gctatttcgt tgtccttaac gcggccttgg ccctaggctc agtgaaattc
     6901 ttcttggggt tgtcccggcc gacatggaat cgcacgaggc ggccggagga agaggcgatg
     6961 cgagcagatg actggtctga ggtgatgccg gacgatgcgg ttgaaaaaca gcgtccggcg
     7021 gtttagggca tgtcggctct ggatctcagc gactcgtcgt cactcggaga ttcacagggg
     7081 ccgccggcgc ggcgtagtca cccgatccgg tcagcaagtg agacggcacc ttcactaaga
     7141 aggcatccag ccgattcccg cccatgttgc tggtccagat gaagtacctg ccgctgatat
     7201 ccagatttcc tttgggatat ttcgcgtatt cgcttcctcc tcccggagca ttcatatctg
     7261 tcatcacggg cgcgacgatg agttgatcgt gggaggcatc cagtcgaaag catacaactt
     7321 cattctgtac tgatccataa tctgcgttgc tgccgcacgc gaactgttgg gacatgggaa
     7381 tggtggattt ggcattcaaa tgggagacat ggttgacagg attcatgttc cagttattgc
     7441 tgtaaaatac cgcggggcct ttcacgatgc cggcaagatt gaaggtgatg gctgcgcccg
     7501 gcaaggaatt ccagttatcc attccgagag catagccgaa ccccatatcg gcatgtccta
     7561 agccaccgtt ctgatccatc acgacgcttt gcttccctgt ttctagatcg atgaacacgt
     7621 tctcgagacc gtattgccca tccctatttt cctggatcat cagatatttt ccgctcttat
     7681 cgaggtggca ttcgtcgtag gtgcccacct tagggaagaa cgtgaatttg cgagtcgttt
     7741 cattgaagac gaaacagcct aaatcctcac cggtggcgga gacgcgtaac gccccgctgt
     7801 gaaccatatc gttgttgctg gaatgcgtct gccacacgta acggccgctt ccgaattgcg
     7861 ctgtaatatc gaacaccgta tcggagactt tcgagacgac gtcgtaccgc aacagtcgtg
     7921 ggccgtcgtt gatgtagagt ttgttgggtc ggctggcgct gaaataccat gtttcgccgg
     7981 attggcttct gaatggactt cccgccggaa agagcggacc aacctttgta atgacatcgg
     8041 tcgccttatt cagcttgaac agcgtcggac cttgtccgcc cctggccgtg ctcaatccta
     8101 caaagatcca catgtccgcg ctgctctcgt gggcattggt gttccgccaa tacgagtagc
     8161 cgatataatt cacgcagtct gcgccaccgc agtcggacgc gtccgtaatc cgaatgcctt
     8221 tggtcctgta gggggcaggg aaggtgaagg tgccgcgtgc cggaggaacg aaggattgaa
     8281 tctgggaggc cgtgtaccga gcacgcgtat acgtaccttg tttttcgatc cagccgccgg
     8341 ggaatgacgt ggtggtttgc cccacgaccg cggtggcaac gagcagcacg agcgaacaac
     8401 acacggcggc acgtaggatt gcggcacttt ttgtgacgaa caacatggcc ttcctcctgg
     8461 tgggtggttc tccagaggga cggtaccatc gtcagacaaa ccctcagtga agatgcgtaa
     8521 atacaatgtg cttagatgga aagaactaca tgaagagccg gtaaaatcgt gatggcagcc
     8581 gtggaacgaa ttccggcata gaagttattg cacgatgccg aacaacccga cgacagctga
     8641 gcatgggagc gagggaggga tgactatgga aatcgcagag cggagccgac aggattttgc
     8701 ggaggaacat gtcgaagagg tccggcaggg aaggcgattt gagtttgggg ataattggta
     8761 caggttcctg cagaatttga aggaagaaca gattgtgtcg gcggagacct cccttcgtga
     8821 aatgctggag atctcggatt tgaccgggaa gaaatttctg gatgcgggct ccggcagcgg
     8881 gctattcagt ctggcggccc gtcggcttgg cgctcaagtg cattcattcg attatgaccc
     8941 gcaatcggtc gcctgtacga aggaactccg ccgacgatac tcacagaatg atccgtggtg
     9001 gcggatcgag caccaatcag tcctggatca agattatctc tcctcccttg gtcggttcga
     9061 tgtggtgtat tcgtggggcg tgcttcacca tacgggtgcg atgtggaagg cattggacaa
     9121 cgtgcaccgc tcggtcgcga gcggagggct gctgttcatc gcgatctata atgatacagg
     9181 aacgcagagt gcgcgctggc gccgcatcaa acagctctac aatgccttac cccgccctct
     9241 tcggtggccc tatacggcgg tcgttgccgc accattggaa ttcaaagccg ttctcagcgc
     9301 gttggcttcc gggcatccca gcagctatat caggcgctgg acggacaaga acgaaagagg
     9361 gatgactcac tggcgcgatg cggtcgactg gataggcgga tatccgtacg aagtggcaaa
     9421 accggagcag atattcgaat tttacaaggc ccgaggcttc agcctccgtc gattgaaatc
     9481 caccgtagga ttgggatgca acgaattcgt cttccggaaa gacagggagg tgaaggcgtg
     9541 ataggccccc aaaagtttat tccgtgggag caggatcgag acggtacccg acgttacgca
     9601 ccgtgaagat acggcgacct tcggcgccca gcttattgcg caggctacga atgtggacgt
     9661 cgatcgtgcg ggactgcaga tccgcgtcct tgctggctcc ccataccgat tcgaagagcg
     9721 catcccggct caggacgcga ccgttcgtca tgagtagcgc acgaagtaag ctaaattcct
     9781 tcgacgtcaa gtgaatgggc ttcccgcgaa gcgtcacctg gtaccgatcg agttccattt
     9841 ccaacgagcc gaccttcagc acccgagccg gatcggtggg tgcggatcgg cgtaaacgcc
     9901 ccttgactct ggcgatcagc tcgttggctt gaaagggctt tgtgatgtag tcatcagcac
     9961 cgatctccag gccgatgacc ttgtccctct cctcatcctt tccgctcagg acgatcaccg
    10021 gcagatgagc ggtaataggg tcctggcgta tgcggcgaag tacctccaac ccgtcgagac
    10081 ccggtaggat caggtcgagc aagatcaggt cgggtttttc acgacgaact gcttccagcg
    10141 cctccgggcc gtcctgagcg acggccacct cgaatccttc gcggtgcaag agactgcgaa
    10201 ggaggtcgac gacgctgtcc atgtcttcga cgacgagtat ctttgccatg agcggctgtg
    10261 aaaaaatcta gggttcgatc aagcggcggc ggatggagta tcgcacgaga ctggcggtat
    10321 cgtgaagatc caacttgtcc atgatgcgat gccggtgcga ttccgccgtt ttcacgctga
    10381 cacctaacat gccggcgact tttttcgtcg acatgccttg agcgaccagt tgcaggactt
    10441 cccgttctct cgtggttaac ggatctgagg gaatgtcgct cttggcaagg aatgcttgaa
    10501 tgaccgtctg ggatatgccc gggctcaggt aaaatgatcc tttggacacg tcgcggatcg
    10561 catgcacgac atcggtcacc ggttgcgatt tgagcaagta gcctttgacg cccactttta
    10621 gcgcttcaag tacgaaggca tcgtctgtgt gcatcgtgag taagagcgat tttgtccccg
    10681 gcgacacccg gcgtatctcg cgcacaacgt ccagaccatt cagtaacggc atcatgacat
    10741 cgatcaccgc cacatcgggt tgcatctcct ccgccatgcg gacggcttcg cgtccatcac
    10801 cggcctcgcc cacaacggta accccatagg tctcgatcaa ggccttcaca ccctgtctca
    10861 gcagcgtgtg atcatcggcg agcagcagcc gacaggatac gagcgatggg gcaggctccg
    10921 cgtttcgatt ctcgagccga ctcgttctag gcgacggcat gagcggtgct gaactgcgag
    10981 agatcaaaga gagatccttt cgttgcggtc cttcacccac gcgtaataac ccctggacat
    11041 ccttgcgaat tatccgccgc tgactgcacc gtgtgcgatc ctcgtccagc tgatgccatg
    11101 cgtcactctg tcacatgacc agcccagttc gcacctaggg aaccgcctag ttggaccggc
    11161 gcgggagttt gcttacagtg accacattca gggaggacct gtggcaacca tgacagcaac
    11221 ggttcctacg ttgagcgcgt gctgtgtttg caaacacgtt gccgagccgg tctcgtcttc
    11281 tattcattgg acgccgatgt cggcgtatct tgagcggcat catctgcgtc ttagcgatgt
    11341 ccggttgagt cacacgtact gcccggtatg ctacgagcgc caggcccgag cctggtccct
    11401 tccagtcatc aagccttccg tgagaatgac acgccgagct gcttgaactc cgctccttct
    11461 gcttcacgac ccgccctaca cattgccact gagagtagac ctgtctgcta gggttcatgc
    11521 gcgcctgcgc cggcgcgcgc caagaggttc gcgaccattt gtcccacagc cggttgactt
    11581 cggatatgct ggctaaaaaa gattgagccg gcggcaaaca cgaacgcgct acctcgttca
    11641 taaatggtcg attcgtggcg actgggtatg gcggggatgt tgttcggcac aacggatgac
    11701 gccaggacag taaggcctga cggggtcagc ccattgttga cgagtgcatc ccattcggta
    11761 aagacgagat tcggaatccg gctcccattg ctgaggccgg tcccggcata cacccaatga
    11821 ttcgcattgg tcacaaccca gtcggacggc tgagtgatga aactgccgtc agacatgaca
    11881 ccgaccaact gattttcagg gcgattcacg ggggggttgc gaaaagtgga cgtgaccagg
    11941 cttggattgg tcaatcgcat ggggtcttca gctgcgtggt ctttgtagcc gacgatgacg
    12001 cggtcgttat tttcatagcg gatttgccaa tacaatgtgt tccctgtgag aaacgccagg
    12061 ctcgtgccgt ttgcgaccgc gctttgtagc cggtcgaact ccgccatcgt tgcgtactcc
    12121 tcgtgcccga tgatggtaaa cagccggtgt gctccgattc gaccgttggc gcctccggta
    12181 tcgctgctgg cttgatactc gacatcgtag ccgtgatcct cgagccatat gaccgcttcg
    12241 tgttcctgat agagaaaggt gccagtgccg ttcccatatt gcctcgcaat gggcctgtcc
    12301 aacgaaactt tacgcgcttt ccctcccgat agcccgtgag aggactcgta gaggctttcg
    12361 ccgccgtagt tgttataggc ctggtaggta gccgtgggga gctgcacgcc gacctcggca
    12421 tcacgggtgt catcgcgaac gatgaagagc gcggcggttt gccggccatc gtctttggtc
    12481 agcatgacca gatagacgcc agtgagccat ggggtaccgt cgctgcgcct ggttggaaag
    12541 gagaaggtag caggccatcc ggccaccatg agaccggtca cggggtcaaa cgtgagatct
    12601 gcctggcgcg gggcagcatg gggtccgcct tccgcataga gccgacctcc catccctccg
    12661 taacttccca tcctaaacac ctgccatcga aaatttccgg gcggatttgc agagacagaa
    12721 atacgcacct cagcaccacc tgggtagctt tcacggtccg gataggcagc aatttcaccg
    12781 ccgttctctg ggttttgaat acgccaggcc gtcgtgcccg gtagagtgtt ttcccgtgcg
    12841 atggctttga catcaccagt cacgccggga ggtggactcg aacctcctcc gttactgtct
    12901 ccgcttccgc cgcactgaag ctgcgtgatc aagcagatgg cggcaatagc cgataagctg
    12961 agacggaccc aaaaggtcga agggagcggt gctggggata tcggcatgac gaaaaacctc
    13021 ctcaaaatat ccacacgaat tgatggcgcg cggagagcgg ttcgcccgac ccaagatcgc
    13081 gagccatctc tgcactcgtt cgcacgccaa gccgtcagca agaagcgcac cacccctgag
    13141 ggaaagccat ggccttccct agggcctgtg tagccgatat tgcctagcgg aagtcaggac
    13201 aagctgtccg gactaggaaa ctttcgggac aagttgtcag tgtggggacg gcgccggccg
    13261 gtcgcctgga aggctaaact gttgacgagc gcgagagtct gccgtcacag gtttagggga
    13321 gcctgcaaat actccataga gcgtgggcag cacgatgagc gtcagcaacg tcgagctgac
    13381 gagcccgccg atgacgacga cggccagggg gcgctgaact tcggagccga tcccgtgtga
    13441 aaaggcgagc gggatcaggc ccaacatcgc cacgagagcc gtcatcagca caggccggag
    13501 tcgaagcagg catccgcgca cgatcgcctc gtggtccggc aatccttcgg ctcgcagctg
    13561 gttgatgtag gacacgagta cgatgccatt gagcacagcg actccgaaga gattgatgaa
    13621 tccgacagat gccggcacgc tgag
//
LOCUS       DASSCQ010000045         4181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_155224, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000045 DASSCQ010000000
VERSION     DASSCQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155224"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1740
                     /locus_tag="VFH05_03405"
     CDS             220..1740
                     /locus_tag="VFH05_03405"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET6677287.1"
                     /translation="MKKSTVSSPIDARCHTGVEGLDDVVAEGLPCNCFYLIQGDPGSG
                     KTTLALQFLLEGVRRGEKTLYITLSETKEELLKVAHSHGWSLDSIGLFELSAIEALVR
                     PDAHTTVFHPSEIELTKVTDQLLNEIRNTRPSRIVFDSLSEFRLWAETALRYRRQLLN
                     LKQELAKYQSTVLLLDDKMDNRNIAGDPHVLSLTHGVIEMEQLSPDYGRSRRRLRVLK
                     LRGVQFREGFHDYTIEKGGLRVFPRIVAAEHRSEFRREPVASGYKDLDDLLGGGLDRG
                     TTTLIMGQAGTGKSTLALQYAVQMASCGERSVLFAFDEVRGLVLARAASLGLGLAPHV
                     ETGVISIQQVDPAELSPGEFAMQIRRAVEEGCKLVVIDSLNGYMNAMPGERYLTNQLH
                     ELSAYLNQQGVVTIFILALHGLVSQAEAPIDLSYLADTVVNVRFFEAAGAVRQALSVI
                     KKRSGRHEKTIREFQLESGSGLRVGKPLTEFQGVLSGAPVFHGGSEQMMRQPNGGR"
     gene            1727..3775
                     /locus_tag="VFH05_03410"
     CDS             1727..3775
                     /locus_tag="VFH05_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6677288.1"
                     /translation="MADGNPEERILILAPIGQDATAMARLLHTHGLVGTVCESAADAC
                     RYLTEGAGALLVTEEALELPQMPEVLHDLNAQPPWSELPMIVLTRGGESRLAKLLHLV
                     AQAAGSLTLLERPLGEATLLRSVEVALRARRRQYQVRDLLEQQRIVHKELRESEEKYR
                     SLFESIDQGFCTIEVLFDEHDEPVDYRFLSINPCFERQTGIANAVGRRIREVVPRHED
                     YWFQTYGKIAKTGETMRFEREAAQLHRHYDVFAWRVGEPAERKVAVLFNDITERKTSQ
                     ARLEQFAVELERQVSERTQELMSSQGQLRALATELNLAEQRERKRIAAEMHDHLAQML
                     VLVRLKLGQAKQGPVHRSLEMIKQAEDVVNDALTYTRNLVAELSPPVLHEFGLFAALR
                     WLGEQMQRFQLQVTVQIESTGALRLPEDQAVLMFQSVRELLMNAVKHAVAKQAFLLVE
                     EREGYLQIVVEDQGVGFDLLAMATQQPSPLSSKFGLFSIRERMNAMGGRLELHSSPGE
                     GTKATLILPCSSSQQTSLEPASKVSFRGTPASTNPSRLHGPCRVLIADDHTMVREGLR
                     SVLEGFPDIDVIGEASNGQEAIDLTERFKPAAIVMDVNMPLVNGIEATARIKTRHPEI
                     VVIGMSVNASADNHDAMRTAGASMLLTKEAAVDQLHGAIQQAVREIGQPGRDALGRSR
                     "
     gene            complement(3852..4031)
                     /locus_tag="VFH05_03415"
     CDS             complement(3852..4031)
                     /locus_tag="VFH05_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677289.1"
                     /translation="MFEGLMGSLVSLVIVGAVILGFTTFVAKLLIWIFRTQDDVVEAS
                     AAYDPSIETRLRRAA"
BASE COUNT          881 a         1161 c         1287 g          852 t
ORIGIN      
        1 tttgcttcaa ccttgcttca acgcctggct agcggccccc ttccaggaag cctatctacg
       61 cagattacaa caacgtccgc gtttgagagc aaggacctag gggttcccct agcacaaggt
      121 tcctacagag tcgcggtatg aataggtccc atcagcatct acttaggaca cacacccctg
      181 ttgatccgac ccaaaagcta gaaaggctgg tgcgcgagta tgaaaaaatc gactgtctcc
      241 tctcccatcg acgcacgttg ccacaccggc gtggagggcc ttgatgacgt cgtggctgag
      301 ggattgccct gcaattgctt ctatctcatt cagggcgacc ccggctcggg aaagaccacc
      361 ctggctttac aattccttct ggagggagta cgccgcggag aaaagaccct ctacatcacg
      421 ctctcggaga cgaaagagga gctgcttaaa gttgcgcact cgcacgggtg gtcgctggac
      481 agcatcggtt tgtttgaatt atccgcgatc gaagcactcg ttcgtcctga cgcgcacacg
      541 acggtgttcc atccctccga aatcgagttg accaaggtga ccgatcaatt gctgaacgaa
      601 atccgcaaca cccgcccttc gcggatcgtg ttcgattcgc tttccgagtt caggctgtgg
      661 gctgaaacgg cgttgcgcta tcggcgacag cttttgaatc ttaaacagga gcttgccaag
      721 taccagagta cggtcctgct gttggacgac aagatggaca ataggaatat cgcgggcgat
      781 ccccatgtgt tgagcctgac gcatggcgtc attgaaatgg aacaactgtc tccggattac
      841 ggccggtcgc gccggcggtt gcgtgtgttg aagctccgcg gcgtccaatt ccgggaaggg
      901 tttcacgact acactattga gaaaggcggc ttgcgcgtct ttccccgcat cgtcgcggcg
      961 gagcatcgct cggagtttcg cagggaaccg gttgcgagcg ggtacaagga tttggacgac
     1021 ttgctcggcg gcggcctgga ccgcggaacg acgacgttga tcatgggcca ggccggcacg
     1081 gggaaatcga cgctggcgct gcaatatgcg gttcagatgg cctcgtgcgg ggagcgaagc
     1141 gttctgttcg cgttcgatga agtgcggggc ctggtgctgg cccgagccgc ttcactgggt
     1201 ctggggcttg cgccgcatgt cgaaaccggc gtcatcagta tccagcaagt ggatcccgcc
     1261 gaactttctc cgggagagtt tgccatgcag atcaggcggg cagtggaaga gggttgcaag
     1321 ttggtcgtga tcgatagtct caatgggtat atgaacgcga tgccagggga gcggtacctg
     1381 accaaccagt tacacgagtt gtcggcgtat ctgaaccagc agggcgtcgt gacgattttc
     1441 atcctggcgc ttcacgggct ggtgagtcag gctgaggccc cgatagacct cagttatctc
     1501 gccgatactg tggtgaacgt gcgctttttc gaagctgcgg gcgcggtcag gcaggcgctt
     1561 tcggtgatca agaagcggag cggtcgtcat gagaagacga ttcgtgaatt ccaattggaa
     1621 tcgggcagcg gcttgcgcgt cggcaagcca ctgacagaat ttcagggtgt cctcagtggt
     1681 gcgccggtgt ttcacggcgg ctctgaacag atgatgaggc agcccaatgg cggacggtaa
     1741 tccggaagag cgcattctta ttctcgcgcc gatcgggcag gacgcgaccg ccatggcgag
     1801 gctgctgcac acgcatggat tggtcggaac ggtctgtgag agcgctgctg acgcctgccg
     1861 atatctgacc gaaggcgcgg gggccttgct ggtcactgag gaagcgctgg agttgccgca
     1921 gatgcccgag gtgctccatg atctgaatgc gcagccgcct tggtcggaac tgccgatgat
     1981 cgtgctgacg cgcggcggag aatcgcggtt ggccaaactg ctacatctcg tggcccaagc
     2041 cgccggcagc ctgaccttgc tcgagcggcc actcggagag gccaccttgc tgcgttcagt
     2101 ggaagtggcc ttgcgagcga gacgccgcca gtatcaagtg cgggatttgc tggagcagca
     2161 gcgaatcgtt cacaaggagt tgcgcgaatc ggaagaaaaa taccgatcgt tgttcgaatc
     2221 catcgatcag gggttctgta cgattgaagt cttgttcgat gagcacgatg agccggtgga
     2281 ttaccgcttc ctttctatca acccctgctt cgagcggcag acggggattg cgaatgccgt
     2341 gggcaggcgc atccgcgagg tggtaccgcg acatgaagac tactggtttc agacctacgg
     2401 caaaatcgcg aagacgggcg agaccatgcg attcgagcga gaggcggccc agctccatcg
     2461 ccattacgac gtgtttgcgt ggcgcgtcgg agagccggcc gaaaggaaag tggccgtgct
     2521 cttcaacgac attaccgagc gcaaaacctc acaggctcgc ttggagcaat ttgccgtaga
     2581 actggagcga caggtatctg agcgcacgca ggagctgatg tcctcccagg gccaacttcg
     2641 ggccctggcg acggagttga acctggcgga gcagcgggag cgaaaacgga tcgctgccga
     2701 aatgcacgac cacttggcac agatgctggt gctcgtgcgg ttgaagctgg gtcaggcgaa
     2761 acaagggcct gtccatcgct cgctggagat gatcaagcag gcggaagatg tcgtcaacga
     2821 cgcgctcacc tacacgagaa atctcgttgc ggaactcagt ccccccgtgt tgcatgagtt
     2881 cggtttattc gccgcgctca ggtggctcgg tgagcagatg caacgctttc agttgcaagt
     2941 caccgtgcag attgagtcga cgggagccct ccggctaccg gaagaccagg ccgtgctcat
     3001 gttccagtcg gtgcgcgagt tgctcatgaa cgcggtcaag cacgcggtcg ccaaacaggc
     3061 gtttctcctc gtcgaggagc gggagggata tttgcagata gtcgtcgaag accagggagt
     3121 gggattcgat ttgctggcca tggcgacgca acagccgtcg cccctctcgt cgaagttcgg
     3181 cttgttcagc atccgcgaac gcatgaacgc gatgggcggc cgcttggagc tccattcctc
     3241 gccgggagaa ggcacgaaag ccacgctcat cctcccgtgc tctagcagtc agcagaccag
     3301 tctggaaccc gcgtcgaagg tgtcgtttcg cgggactcca gcctctacaa atccgagtcg
     3361 actccatggc ccctgtcgcg ttctgatcgc cgacgatcac acgatggtgc gggaaggatt
     3421 acgttccgtg ctcgagggct ttcccgatat cgacgtgatc ggcgaagcgt ccaatggaca
     3481 agaagccatt gacctcaccg agcgattcaa accggcggcg attgtcatgg atgtcaacat
     3541 gccgctggta aacggcatcg aagccaccgc cagaataaag accaggcatc cggagatcgt
     3601 ggtcatcggt atgtcggtca acgcctcggc cgataatcac gatgccatgc gaacagccgg
     3661 cgcctcgatg ctcttgacca aggaagcggc tgtcgaccag ctccatggcg cgatccaaca
     3721 ggccgttcgg gaaattgggc agcctggacg agacgcattg ggcaggtccc gttagggggc
     3781 tccgtccctg tttgcgacga cgaccttcat tccacaggtc tagaaattgg caccgccctc
     3841 acgtcttttg gttatgccgc cctgcgcagc cgcgtttcga tcgacggatc atacgcggca
     3901 gacgcttcaa ccacatcgtc ctgtgtccgg aaaatccata tgagcagctt cgccacaaag
     3961 gtggtgaagc cgagaataac cgcgccgacg atgacgaggc ttacaaggct tcccatcaac
     4021 ccttcaaaca taaggcacct cttgagaagg ttgtgttgag gttctgcact attctacaaa
     4081 caaaggccaa aagtgggagc aagagcttcc ctaggcgttt cctagggcgc caagcattca
     4141 gcgaggctca atagttcagg acgtcgtttc attgttgttc t
//
LOCUS       DASSCQ010000046        28568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_613540, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000046 DASSCQ010000000
VERSION     DASSCQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 28568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28568
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613540"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFH05_03420"
     CDS             complement(<1..593)
                     /locus_tag="VFH05_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6677290.1"
                     /translation="MRMSALVQRNSGRTFCAGLMLAALGLASVIPGTARAGAATEAMK
                     GTIEEVLRILADKDLKQPAKANERRQLLEKVVGERFDYQEMSRRSLGAPWGNMSDKEK
                     QEFVSLFQTLLVNTYADKVESYSGEGVQYVNERTEKEYAEVRTKVLTGKTEIPLDYRL
                     LNKGSAWRVYDVVVDGVSLVNNYRGQFSKILRNGSYA"
     gene            complement(645..1088)
                     /gene="mlaD"
                     /locus_tag="VFH05_03425"
     CDS             complement(645..1088)
                     /gene="mlaD"
                     /locus_tag="VFH05_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HET6677291.1"
                     /translation="MEKTKLEFIVGVFVLVGIICLGYLSIKLGKLELIGGDVYEVIAQ
                     FNSASGLKAGSAIEIAGVEVGRVRAIRLNEDRAAVVLAVDNTVKLYTDTIASIKTRGI
                     IGEKFLALSPGGGGDPLKPGDTIRDTESGLDLEELVSQYVHGNVK"
     gene            complement(1108..1863)
                     /locus_tag="VFH05_03430"
     CDS             complement(1108..1863)
                     /locus_tag="VFH05_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6677292.1"
                     /translation="MIRLVGVEKTLGGQPVLQGLDFSAPAGKLTTVIGKSGEGKSVLL
                     KHMIGLLQPDRGQVWVGDAEISRLRGKALNEVRKRFAMLFQGAALFDSLSVFENVAFP
                     LREKLGLKGTEVTRRVDEKLEQVGLAGMGHKFPAELSGGMKKRAGLARALVMEPEIML
                     FDEPTTGLDPLMAKSIHDLIVTMQRRFKFTAVMVSHEIPEIFAISDWVAMLRKGKIAL
                     MAPAADFVKTTDPEIQEFISVSGPIRLSGVAAG"
     gene            complement(1869..2633)
                     /locus_tag="VFH05_03435"
     CDS             complement(1869..2633)
                     /locus_tag="VFH05_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HET6677293.1"
                     /translation="MMVKRLGAWALDSIQEMGRMLLFVLSSFAWLVRPPLRFVQIVKQ
                     LHFVGYKSTFVVVLTAGFTGMVLALQGYYTLRKFGSEGLLGSAVALSMIRELGPVLAA
                     LMVTARAGSAMTAEIGIMRITDQIDALDTMAINPLQYLIAPKLVAGLIGVPLLVAIFD
                     VVGIYGGYLVGVELLGGNGGAYWNSIESAVEWKDVYGGILKSISFGLIVSWVCCYKGF
                     FTKHSAEGLGTATTEAVVLSSVLILVWDYFLTSVLL"
     gene            complement(2630..3397)
                     /locus_tag="VFH05_03440"
     CDS             complement(2630..3397)
                     /locus_tag="VFH05_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677294.1"
                     /translation="MATRWELAAVRRALPVNHRIDIGGVGCFIGRQAARSYWLIHTGI
                     GPEAAEAAASAVLNRERMALAVSLGFTGALASGAAISDVIVGTRVVSGTFDGLWHESG
                     QPVLCHEGAADAARAAAADIGLEAHTGAVLSVSTVICGAAEKQRLARLTGAIAVDMES
                     AALGTAALAQGVPFAVCRTVSDIVTEDLPLNFNLFLMRSGWMRGIAAIVMRPSSLIGL
                     NRLRRQSRLAADRLTALSSAYAARGFGVVPLINTERA"
     gene            complement(3472..5643)
                     /gene="shc"
                     /locus_tag="VFH05_03445"
     CDS             complement(3472..5643)
                     /gene="shc"
                     /locus_tag="VFH05_03445"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET6677295.1"
                     /translation="MPMNLIRTILDRLSDSFFISVPDRFTADPLAGPSLRLVSDKSAV
                     VADAPSRRLSSQAGSQLDAIDDAIRRSQAWFLSRQHPTEGYWVAELEADTTLTAEYLM
                     LRRLLDQVDPERERKAVSYLRATQLPDGGWPIYYGGPSEISASVKAYFALKLCGISGD
                     EPFMLRARNCILGKGGVVQANVFTKITLALFDQYDWEGIPHMPVELMFLPKWFYFNIY
                     AISYWSRAVLIPLLIVFAHRPVCRISKDQGIEELYASPRTEIRYWKFPPFNKDQKWLT
                     PHNVFVALDALLKIYDHMPLLWLREKALHQAATWMLEHIKGSGGLGAIYPAMANSIMA
                     LQCLGYQVDDPLVRKALREIEDLEVYDTVSIGDKRVEAMHLQPCHSPIWDTSLLMNAF
                     IEAGMPQDHPALQRGAAYLMSRQTKTVGDWKVSSPKAEPGGWYFQHENELYPDVDDSA
                     VVLMALAKVKVGDEAELRESIRRGAAWVVAMQGSDGGWGAYDKDNNRIVFNYIPFADH
                     HALLDPSTADLTGRCLEMLAALGYDRSHPAVAPALAFLKHEQEPDGSWYGRWGVNYIY
                     GTWSVLAGLRAIGEDLSLPYIRRAVSWLESKQNPDGGWGESCLSYADPACAGRGPSAP
                     SQTAWALLGLMSAGMCDSFSVARGIQFLLRHQMKDGSWEEVRHTGTGFPRVFYLRYHW
                     YCQYFPLWALAMYRNLRTRNTMRADEVRNQILASGCYRFDC"
     gene            complement(5831..9493)
                     /gene="smc"
                     /locus_tag="VFH05_03450"
     CDS             complement(5831..9493)
                     /gene="smc"
                     /locus_tag="VFH05_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET6677296.1"
                     /translation="MYLKSLEMVGFKSFAEARLEFPNGVTAIVGPNGSGKSNVVDAIL
                     WVLGEQSTKTLRSEKMEDVIFNGTELRKPLGMAEVSLVISGLDQVKVDSQNGLPGQLS
                     EYQDLMITRRLYRNGDSEYLINKTQCRLKDIRSILLDTRAGTKGHTVIAQGQIDQILN
                     ASPQDRRELIEETAGIVRYKKQKAEALRKLDATQQNLLRVRDIIAEVKKQLNSLERQA
                     RQARSYQVLQQEARTLEVQLLTNELRRLRQTIGEIERELATLSGQESEQAALQARLSA
                     ELEGLKLAIADTGLVIVQRREELSHIEQRQAQALTAAEVERHKGELYEQQRQQGAEDR
                     ARLLSEQTAASEAEAALCAELTRLEEVCREREHALAELDHEMKGLVHQRVASLQEEDR
                     GRADVLNLTVLVANTEQNLEQLTARLREISERDGRLTREREEVQSQHDTALEKQSRLT
                     EACRGSDGLVVDLRRQHHELEQQSVATGVKLVEVEQQVSAGSEELAAVDSHLRALQGV
                     LREDMGYGRSGNEEATALKTCQGVHEAVAEWLIVPAGLDRAVEAVLGERVRGWFVDGP
                     QSASQAIAFLMEKDLGRGTFIPQAPRWMAPERSHDWWTGMDGRAGVIGRAVDLVGISG
                     SRNAARDYLFDRVVFVETLEDAMALWHEQERTGADGATFVTRAGELVDAAGVVTGGQV
                     SSSGGLLQRRREVMELEVRRTTLVAEVEQARQRRESLQAESHFLREEGRRVADALREE
                     EMNALSLQKDASGIQQLLGDLTQRIETLLGDAQRGSLEVRRLEGEATSAEAQLAQWIA
                     EKNGQEGALQAVRERVVHLDQAIHGLQQRLTEAQLSVQGARNTCEHRQADLQRVRQEQ
                     QDTAVRIGSLDQQLELLMASAEQSRAERDRQEAMCREIGESAEQLKARIVEAQEGQAR
                     DMAQAHSIETRLDEVRKLVSGLQESRLTFEVRRAEVRTQLATVESTLSGTYQMDPGSV
                     CESGAPALLEPEAQAQVSPSEDEIRQQLLKIRERLDRMGPINLAAINEHQELEERHRF
                     LSTQEQDLSNSIASLKEIIQRINRTTKDMFATTFAELQEKFSEVFAKFFRGGRAELQL
                     VDVEADAQAEHPGPQEPGVEIVAQPPGKRLKSITMLSGGEKTLTAMALLFASFLIRPT
                     PFCILDEIDAPLDEENIGRFTSVLCELAEGAQFMVITHNKRTMGIADSLFGVTMEEPG
                     ISKLVSVRLNELQTVS"
     gene            complement(9655..10614)
                     /gene="ispH"
                     /locus_tag="VFH05_03455"
                     /gene_synonym="lytB"
     CDS             complement(9655..10614)
                     /gene="ispH"
                     /locus_tag="VFH05_03455"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET6677297.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRHKGAVFVEELNEVPEGSVVIFSAHGVAKSVWDEANRRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYDLILIGHAGHPEVIGTLGQVPDKFHLVSSVEDVERLQVHNTTNLSY
                     VTQTTLSVDECRDIVGALHKRFPHIKGPHQEDICYATQNRQNAVKELSKLVDVILVIG
                     SPNSSNSNRLRELGEHCGISSYLIDSAADINPEWVKNAKAIGITAGASAPEVLVEEVV
                     AYLKTYDSAEVEDLTVIEEDVEFLLPRELITIAPASKSVAASR"
     gene            10849..11550
                     /locus_tag="VFH05_03460"
     CDS             10849..11550
                     /locus_tag="VFH05_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677298.1"
                     /translation="MASVPAKKILIIEDERDILQLVKLYLEKEGFRTVTASTGAEGLN
                     SAKQSKPDLIVLDLMLPEIDGLEVCKRLRSAPETAMLPIIMLTAKAEESDQVIGLELG
                     ADDYVTKPFSPKALVARVKALFRRTDRSRQESQTAYQYGKLAMDLDRHEVTVQGKDVP
                     LTAKEFGLLEHLLRHPGRVLTREVLLNQVWGYDYFGTTRTVDVHVRRLKQKIPLLDEA
                     ILSVKSLGYKLKVLD"
     gene            11579..13381
                     /locus_tag="VFH05_03465"
     CDS             11579..13381
                     /locus_tag="VFH05_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677299.1"
                     /translation="MSLSIRWKVTLAILFALLCGFVIAGVLAARSLAEQELAQSGEAL
                     QVSTHLIALDLRPLVTASSLPPTPQLQTIVGELSQRARARVTLIDASGRVLADSAVRD
                     GDLAGVENHRTRPEIEQAVATGSGTDLRASHTTGERTLYRAVRLGDPGAASAPLYLRL
                     GLPMTALEHELTILQRNLTLAFGSAFLIAVAMSVWLARSLTRPLSDITAAARQLAGGN
                     LSVAVRTSSRDEVGLLADTLNRMTRELRSKIEELSEDRSQLLAMLTSMVEGVMVLDSK
                     GRILQVNPAWERMFGVTRNEARGRQSSEVFGHPKLNALVSAVLAARTGQEGEILLPSG
                     RCLNVEASVAGGEQDNEACAVLVFHDITELRRLEHIRKDFVANVSHELRTPLTSIKGY
                     VEALLDGGKDDPETSVRFLDIILKQSDRLNLILEDLLQLSKIESGQVQFKQEPLHIGS
                     VIERTIAMIKPLADKKQHRLIAQVDADLPLITGDQERLVQVLANLLDNSIKYTPEGGQ
                     ITLSARRLPATRTGPGDGVELMVSDTGIGIPEADRPRVFERFYRVDKARSRELGGTGL
                     GLAIVKHIVEGHGGQIHVEANAPSGSRFVVRLPA"
     gene            complement(13398..14390)
                     /locus_tag="VFH05_03470"
     CDS             complement(13398..14390)
                     /locus_tag="VFH05_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET6677300.1"
                     /translation="MPELTGMLLLVVVLALLFDFSNGWHDSANAIATVVSTRVISPFV
                     AVIGAGVLNIAGAFMSTAVAKMVGSGIVDPAGVTQHMVAAALAGAIFWNLFTLLLGLP
                     TSSSHALIGGLVGAALTHGGWDTVKVAGLRSVLEAMVLSPFFGFAMGLTLMVLISWLF
                     FRVTRAVATRLFKRLQLISACFMAFSHGANDAQKSMGIITLALLSAGQIPSAEVPTWV
                     IGACAVAMGLGTAVGGWRIVRTLGMKIVKLEPVHGFAAETAAATVLMFTAHIGLPVST
                     THTITSSVMGVGAIKRLSAVRWGVTRRILYAWLFTLPGAAIISTAIYLIMGLFL"
     gene            complement(14515..15132)
                     /locus_tag="VFH05_03475"
     CDS             complement(14515..15132)
                     /locus_tag="VFH05_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET6677301.1"
                     /translation="MFGLIPKEERFFELFKNAAHNMIEGSRLLKDMMEHFESPVDQAK
                     RIKDVEHVGDGITHDIALRLNQTFITPIDREDIHDLASALDDILDVAEAIADRFVLYK
                     VTTPTDKAVKLADVLYQASVAVGAGVDRLGLSHPDIKECSVQVNSLENEADRISRDAI
                     SELFEKETDPVAVIKWKEIYEGFEAGTDRCEDVANILERIALKHH"
     gene            complement(15230..16222)
                     /locus_tag="VFH05_03480"
     CDS             complement(15230..16222)
                     /locus_tag="VFH05_03480"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET6677302.1"
                     /translation="MIRSRLIGTGSYLPERVVSNREAGAALGIDPEKVMQLTGIRERR
                     WAEASQASSDLAVSAARDALQAAGLPLGAVEVIIVSTTSPDMAFPSTACHLQRKLGCA
                     SVPAFDVAASCSGFLYGLSMADAMVRSGQAKTCLVVAAEVKSRTLDPSDSDTALLFGD
                     GAGAAVVHGEPDADQPSRGLLGIRLHSDGARHDLIRIRAGGSRRPASHETLDGQEHSL
                     RMQGAPLFRLAVRHLERGLLDILKEFGVDKGDVSQFIMHQANGRILAQLASRLAIAPD
                     SLCSVIERYGNTSSASLPIALDHYVRSGRVRPHDLIALGSFGGGLTWASGLIRW"
     gene            16346..17287
                     /gene="bamD"
                     /locus_tag="VFH05_03485"
     CDS             16346..17287
                     /gene="bamD"
                     /locus_tag="VFH05_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET6677303.1"
                     /translation="MKINLVQTDLLPAARSLSVALGMAALCLLMACSSSPKNGDATSK
                     KALSGTDESIFLGDTIEKNYDPNVIMKRGEAFFEKEEFAEAIVEYNHFLDLHRSHMLA
                     PYAGFRIGESHMKRAKGIDRDPEPIQKAIDSFERLRRDYPGSRYDSEAVDRIQECHDL
                     LAQMHLFVGQFYYRRQSYLAAAHRFEQIMKIYPDKSVAPDALYFLALSYHDLGADDWA
                     SEKLTLLAEKYPNSTHSNDGAKLLAKIIKSQPSVLVAKKPEAAAPIPEPALAAQESSR
                     SVPAGGVTPQVANGLMRLPSATAMGQGFVNCRLGAWC"
     gene            complement(17545..18291)
                     /locus_tag="VFH05_03490"
     CDS             complement(17545..18291)
                     /locus_tag="VFH05_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET6677304.1"
                     /translation="MSESLTQISMIAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDANARSQFKRAIVVNALLFIAGFSVVFIAFGASASFVGQLLMTYQEHIRRIGGLLI
                     VIFGLYLLGILNINFLQMEHRFQFRNRPVGYLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTDSLLDGVFLLTSYSLGLGLPLFLTALGVDRFLAYFKQARAYLWGVSTVSGVLL
                     VIVGVMIYANSLTMITSFLERHGIGWYLGQ"
     gene            complement(18301..18837)
                     /locus_tag="VFH05_03495"
     CDS             complement(18301..18837)
                     /locus_tag="VFH05_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6677305.1"
                     /translation="MKPLVTAALLSVFLTMALSASSQAGSAASVAQRGMVKPGEPAPN
                     FQLRDMNGRIVSLSELRGKVVLLNFWATWCGPCRVEMPAMERLYRTYDRKDFEILAVS
                     TDAQGVAVTRPFQEENKLTFPILHDADFRVGLSYGARTLPMTFMVDRQGVVRQHIFGA
                     RDWEAPEAHQLIEMLMKS"
     gene            complement(18834..19469)
                     /locus_tag="VFH05_03500"
     CDS             complement(18834..19469)
                     /locus_tag="VFH05_03500"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6677306.1"
                     /translation="MTEQTIPSNVTSLPSRGRSRVLILAVGTAILIAVFTLVWLQSAK
                     YEPLAVGKPAPDFSLTDLNDKQYRLSDFRGKVVFLNFWATWCKPCREEMPSMEILNKN
                     FEKDGLVMLAVSIDRVTTTKDIPPFVKGLNLTFPVLVDSWGRTDKPYKRMGVPETFII
                     DQQGIIQEIVIGPRDWTRLDSLQILTKLLNVTPKTAGTRPPLAEASMVDQG"
     gene            complement(19500..20414)
                     /gene="ccsB"
                     /locus_tag="VFH05_03505"
     CDS             complement(19500..20414)
                     /gene="ccsB"
                     /locus_tag="VFH05_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HET6677307.1"
                     /translation="MARSLFLFDMTFWLYLASLTLYVAYLFARRPAMAFAPAGHPAED
                     FDERDGAWAAQLGQVATLVTVFGWMVNTAALLTRAFERLEHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLELRYKIKAIGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGVMYLFKERAEQRGSRSRFYAAFPDLETIDELGYKAIMI
                     GFPMLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGRRAA
                     IYAVFGFLMVIFCFWGVNFLLSGLHAYA"
     gene            complement(20584..22020)
                     /locus_tag="VFH05_03510"
     CDS             complement(20584..22020)
                     /locus_tag="VFH05_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HET6677308.1"
                     /translation="MNSRPETLSEQDTPQTPAPGVGWEEFSRELVEFFASIKLAMFLF
                     LFIAITATIGTVIQQGERAETYIQEYGEDAYRWFLRLGFTDVYHTWWFTGLLGLLCVN
                     SLTCFYKRFPAVWRSMKQDKVSVSLAFIKGMKQQAEVVVQGTKESIAQQLAQHYVGKG
                     YRVLAKNEAGEVTLYATKGVMGRVGAHMAHLSATVIVLGGLLGSYYGFQEFGVCLEGQ
                     TYHIPRGNFDLKVDKFWIDYHENGAVKSYNSTLTVLESGETKITKTITVNDPLVYKGI
                     WFYQSSYGDAWDQIEVARVNIKDKQTEKVVKTIDLEWQKEQTLSDLNLKLAMTDFVAD
                     FAFNSTEKKVYSKTIEHANPAIKLTVNERSTVQATPWIFYQFPDLFEIKDSKYQFELI
                     GYKPKKYTGLQIAKNPGINIVWIGCTMIVVGITLSSFIYHRRLWTKIIPSGASGVTVH
                     VGGTTHKSQIDFQKEFRKLTEKIRTLEA"
     gene            22389..23537
                     /gene="metK"
                     /locus_tag="VFH05_03515"
     CDS             22389..23537
                     /gene="metK"
                     /locus_tag="VFH05_03515"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET6677309.1"
                     /translation="MRNNYLFTSESVTEGHPDKIADQISDGILDAMIAQDKYSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYCDASWGFDCNTCSVLTSIHQQSG
                     DIAMGVDSGGAGDQGLMFGYATNETAELMPMPIVLAHRLTRRLAEVRKKGILPWVRPD
                     GKSQVTVEYRNGKPIRIDTIVVSSQHSPDITSKQIERSIMEKVIKPVMPKGLYDPTSV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPTKVDRSASYMA
                     RYIAKNIVAAGLADKCEVQLAYAIGVADPVSVLVDTKNTEKVSVDNLDKLVRKHFPMT
                     PRGIIDHLKLRRPIFKKTAAYGHFGRNEPEFTWEKTDKAKVLRKDAGL"
     gene            23651..24910
                     /gene="ahcY"
                     /locus_tag="VFH05_03520"
     CDS             23651..24910
                     /gene="ahcY"
                     /locus_tag="VFH05_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET6677310.1"
                     /translation="MDYDVRDIKLADQGRLKIEWAEATMPVLRLIRKRFKRQQPLKGV
                     RVTACLHVTTETANLAITLKAGGADVRLCASNPLSTQDDVAAALVQHEGIPTFAIKGE
                     DNATYYRHIESAIAHRPHVSMDDGADVVSHLHSKRKDLLKNVIGGTEETTTGVIRLRS
                     MADKKVLRFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSVVVVVGYGWC
                     GRGIAMRAKGMGADVIVTEIDPLKGLEAVMDGFRVMPMELAAPIGDFFVTVTGNIHVI
                     RSEHFAVMKDGAIVCNSGHFNVELDIPSLEKQAKKRRVVRPGVEEFTLRKNGHRVSLL
                     GEGRLVNLATAEGHPSSVMDMSFANQALGAEYIVKNYKHLEKKVYPVPPAIDKEIARL
                     KLAGMGVAIDTLTPEQKKYLASWEMGT"
     gene            25770..27275
                     /locus_tag="VFH05_03525"
     CDS             25770..27275
                     /locus_tag="VFH05_03525"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6677311.1"
                     /translation="MLLLVLFMPLLSAYATEPAPVDPRKMTFDAVGYLPSEPDRLVFR
                     NGLVVYLLEDHELPLITIGALLRTGGWLDPPDKVGLAALTGTVMRTGGSGGWSAVEVE
                     DELAQFAGRMNIAIGRHSGSATLDVLKKDLKRGLQLFAGALRTPAFDPAHVELAKLQA
                     IERIRRREDEPESIASREFVKLLYGPSHPSARESSIDSVQRISREDLIAFHANTIHPN
                     GIILGVTGDFRKDEIVASLLEVFGDWEAGAVPEVRIADVPESEAQRAGIHFINKDTSQ
                     THLRVGDLSIKENDTDYIPLAIANDILGGDAGVGRLFREVRTKRGLAYSVGSELSTGV
                     YDQGMWLLWAETKLPSTKEVLGQLVANIERISSELVSDEELDESKEAYVNSWVFDFSS
                     ASKIVSRLMRLEYDGLPKDFFQQVREKVLKVSKDDVLAAAKKHLRLDRVKIIAVGSGG
                     TLSEVLSTFGHVKEIKRSSEGRIHRGRVLLRADDAVREPSYEIDLPPSVAR"
     gene            27241..28437
                     /locus_tag="VFH05_03530"
     CDS             27241..28437
                     /locus_tag="VFH05_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677312.1"
                     /translation="MRSTFHPLSLGDFGISSDQGFLPHDPRESVPDCQPLNDLGHELP
                     KLLSTRTVRPFIEKQHSLLPSIPRSWEEQDYRAAMRILSFAGHAYVWESPDHPAASLP
                     PQLAQPWYEVAQRLGRPPVLSYASYALDNWRRLDCTRPIELDNIVLLQNFLGGLDEEW
                     FVVVHIQIERQAGAGLAGLLQAINGAASDREPEVLMGLRALASSQTAMRDTLLRMQER
                     CDPYVYYTRVRPYIHGWKNSPSLPDGLSYTGLSTYAGPQQFRGETGAQSSIVPSLDAG
                     LGIRHSPDPLTVYLQEMRDYMPPLHRAFLQRLEQATDDLDRPLLAGYVQDRKSSRSEL
                     WAAYCDCVDLLAQFREIHIGYADSYIYRQHQSHVSNPTAVGTGGTPFMAYLQKHLDET
                     KQATTD"
BASE COUNT         6235 a         8610 c         7880 g         5843 t
ORIGIN      
        1 tcggcatagc tgccgttacg aaggattttg ctgaactgtc cgcgataatt gttcacgagg
       61 ctgacgccgt ccacgaccac gtcgtagacc cgccaggccg aacccttgtt gaggagacga
      121 taatccagcg ggatctcggt cttccccgtg agcaccttcg tccggacctc ggcgtattcc
      181 ttttccgtgc gctcgttcac atattgcacg ccctcgccgg aataggattc gaccttgtcg
      241 gcgtaggtgt tgaccagcag ggtctggaaa agcgagacga actcctgctt ttccttgtcg
      301 gacatattcc cccagggcgc tcccagtgac cgacgggaca tttcctgata atcaaagcgc
      361 tctcccacca ccttttccaa taattgtcgg cgctcgttcg ctttggccgg ctgcttgagg
      421 tctttatcgg ccaggattct cagcacttct tcgatggtgc ccttcatggc ctcggtcgcc
      481 gcgccggccc gggcggtgcc cgggatcacg cttgccagcc ccagagctgc cagcatcagc
      541 cctgcacaga acgttcggcc ggaattcctc tgaacgagtg cggacattct catagctcga
      601 ctccctcgtg ctcctaagtc ccgattcagc gcgacggcgc cagatcattt cacattacca
      661 tggacgtact gactgaccaa ctcttctaaa tccaagccgg actccgtgtc ccgaatggta
      721 tcgccgggtt ttaacggatc cccccctcca cccggtgaaa gggcaagaaa cttctccccg
      781 ataatgccac gtgttttgat ggatgcgatg gtgtccgtat agagcttgac ggtgttgtcc
      841 accgccagaa ccacggcggc tcgatcctcg ttgagtcgaa tcgctcgaac acggccgact
      901 tccacgccgg cgatttcgat cgccgagccg gccttcaggc ccgaggcgct attgaactgg
      961 gcgatgactt catagacgtc gccaccgatc agctcgagct ttccgagttt gatcgagaga
     1021 tacccgagac agataatgcc cacgagcacg aacacgccaa cgatgaattc cagcttggtc
     1081 ttttccatcc gtgcactcct tgtcgtctca gcctgcggcc accccgctca gcctgatcgg
     1141 accgctcaca gaaataaact cctggatttc aggatcggtc gtcttcacga agtccgcggc
     1201 cggcgccatg agggcgatct tgcccttgcg caacatcgcc acccaatcac tgatcgcgaa
     1261 aatttcgggg atctcatggc tcaccatcac agcggtaaac ttgaaccgcc gctgcatcgt
     1321 cacgatcaaa tcgtgaatcg acttcgccat caacggatcc agaccggtag tgggctcgtc
     1381 aaagagcatg atttccggct ccatgaccag cgcacgggca aggcccgccc gtttcttcat
     1441 gccgccgctc aattcggccg gaaatttatg tcccatcccg gccagtccaa cctgctcgag
     1501 cttctcgtcc acccgcctcg tgacctcggt ccccttcagt cccaatttct cccgcaacgg
     1561 gaacgcgacg ttttcgaaga cactcagcga atcgaacaat gccgctcctt gaaacagcat
     1621 ggcaaatcgc ttgcgaacct cattgagggc cttgccgcgc aaccggctga tctcggcgtc
     1681 gccgacccac acttgtcctc ggtccggctg cagcaagccg atcatatgct tcaacaacac
     1741 gctctttccc tcaccgctct tgccgatgac ggtcgtcagt tttccggccg gggccgaaaa
     1801 atccaggcct tgcaataccg gctgtccgcc caacgttttt tccactccga cgagtctaat
     1861 cattgccgtt acagcaagac ggaggtgaga aagtaatccc agaccaggat caataccgag
     1921 gacaacacca ccgcttcggt tgtggctgtg ccaagtcctt cggcgctgtg cttggtaaaa
     1981 aatcctttgt agcagcagac ccagctcaca atgagcccga aactgatcga cttgagaata
     2041 ccgccataca catccttcca ttccacggcc gattcgatcg agttccaata ggccccgccg
     2101 ttgccgccca acaactcgac tcccacgaga tagccgccgt agataccgac gacatcgaaa
     2161 atcgcgacga gcagcggcac cccgatcaga cccgccacga gtttgggagc aatcaagtac
     2221 tgcaacggat tgatcgccat cgtatccagc gcatcgatct gatccgtgat gcgcatgatt
     2281 ccaatttccg ccgtcatagc cgaaccggcc cgggccgtca ccatcaatgc tgccagcacc
     2341 ggtcctaact cccggatcat gctcaaggcg actgcagagc caaggagacc ttcggaaccg
     2401 aattttctga gggtgtaata gccttgcagg gcgaggacca taccggtaaa gccggccgtg
     2461 agcacgacaa cgaaggtgga tttgtacccg acgaaatgca gttgtttgac gatctgcaca
     2521 aaccggagag gcggacggac cagccaggca aacgatgaga gcacaaacaa gagcatgcgc
     2581 cccatttcct gaatcgagtc caaggcccat gcgcctagcc gtttgaccat catgctcttt
     2641 cagtattgat gaggggcacc acgccgaagc ctcgtgccgc ataggcggaa ctcaaggccg
     2701 tcaaccggtc ggcagccaga cggctctggc gccgaaggcg gttcagtcca atcaggcttg
     2761 aggggcgcat gacgattgcg gcaattcctc gcatccaccc cgatcgcatg agaaacagat
     2821 tgaaattcag gggcaaatct tccgtgacga tgtctgacac ggtccggcaa acggcaaagg
     2881 gaacaccctg ggccagggcg gctgttccta gcgccgcgct ttccatatcc accgcgatgg
     2941 cacccgtgag gcgcgcgagg cgctgcttct ctgcagcccc gcagatgact gtcgacacag
     3001 acaagacggc gccggtatgc gcttccaagc caatgtcggc agccgccgct cgtgccgcgt
     3061 ccgctgcccc ctcatggcag agcacaggct gtcccgactc gtgccagagg ccatcgaacg
     3121 tccctgagac cacgcgcgtg ccgacgatca cgtccgagat ggcagcacct gacgcgagcg
     3181 cgccggtaaa gccgagggaa actgccagcg ccatgcgttc gcgattcagc acggcacttg
     3241 cagcggcctc agccgcttcc gggccgattc ccgtgtgaat cagccaatac gaccgagcgg
     3301 cctgccggcc gatgaaacag cccacaccgc cgatgtcaat ccggtggttg accggtaacg
     3361 cgcgccgcac ggctgccaac tcccatcgtg tcgccacgaa cagcgcgacg ggactggcgg
     3421 cagatccgcc gagggattgt ccgtccgtga atgagctcgg ccggccttgg gtcaacaatc
     3481 aaaccgatag catcccgacg ccagaatctg gtttcgcact tcgtcggctc gcatggtgtt
     3541 gcgcgtcctc agattccgat acatcgcgag tgcccacagt gggaaatact ggcagtacca
     3601 gtgataacgg agatagaaca cgcgtggaaa ccctgtgccg gtgtgccgaa cctcttccca
     3661 cgaaccatcc ttcatttggt gacgcagaag aaattgaatg cctcgcgcca cactgaacga
     3721 atcgcacatg ccggccgaca tcagcccaag aagcgcccag gcggtttgcg atggcgcgct
     3781 tggacccctg ccggcacaag caggatcggc ataggaaaga cacgattctc cccagccgcc
     3841 gtccggattt tgtttcgatt ccagccaaga caccgcacga cgaatgtacg gcagtgacaa
     3901 atcttctcca atggcgcgaa gccccgccag aacggaccac gtgccgtaaa tataattcac
     3961 gccccatcgg ccgtaccaac tgccgtccgg ttcttgttcg tgcttgagaa aggctagcgc
     4021 gggcgccacc gccgggtgcg accgatcata tcccagagcg gcaagcattt cgagacagcg
     4081 tccggtcagg tccgcggtgc tcggatcgag caaggcatga tggtcggcga atgggatgta
     4141 attgaagaca atgcgattgt tgtctttgtc gtacgcaccc cagccgccgt cagacccctg
     4201 catggccacg acccaggcgg cgcctcgccg aatggattct cgaagttccg cctcgtctcc
     4261 taccttcacc tttgcgagcg ccatcagcac gacggccgaa tcatccacgt cgggatacag
     4321 ctcattctcg tgctggaaat accatccgcc aggttcagct ttgggagagg acaccttcca
     4381 atcgccgacc gtcttcgttt gccgggacat cagataggcc gcgccccgct gcagcgccgg
     4441 gtggtcctgt ggcatccccg cctcgatgaa cgcattcatc aacagcgacg tatcccaaat
     4501 gggcgagtga caaggctgta agtgcatggc ctcgacccgt ttatcaccga tcgacaccgt
     4561 gtcgtagact tcaagatcct cgatttctct gagcgctttt cggaccaagg gatcgtcgac
     4621 ctgatatccc aagcactgga gcgccataat tgagttggcc atcgcaggat aaatcgcccc
     4681 aaggccgccg ctgcctttga tgtgttccag catccaggtg gcggcctgat gcagcgcctt
     4741 ttcccgcaac catagaagcg gcatgtggtc ataaatcttg agtaaggcat ccaacgcgac
     4801 gaacacatta tgaggcgtga gccacttttg gtctttgtta aacggcggaa atttccagta
     4861 gcgaatttcg gtacggggac tcgcgtagag ttcctcgatg ccctggtcct tggaaatacg
     4921 gcaaaccgga cgatgcgcga agacgatcag gaggggaatc aacacggcgc gagaccaata
     4981 tgaaatcgcg taaatattga aatagaacca cttgggaagg aacatgagtt ccacgggcat
     5041 gtgggggatg ccttcccagt cgtactgatc gaagagggcc agcgtgatct tcgtaaagac
     5101 attggcctgc acgacgccgc ctttgccgag aatgcaattg cgcgcccgca gcatgaacgg
     5161 ctcatccccg gaaataccgc acagcttcag cgcgaaatac gctttcaccg acgcgctgat
     5221 ttccgatggc ccgccgtaat agatgggcca ccctccgtcg ggcaattggg tggcgcgcaa
     5281 gtagctgacc gccttgcgtt cccgttccgg atcgacttga tccagaagcc gtctcagcat
     5341 caggtactca gccgtgagcg tggtatcggc ttccagttcg gcaacccaat aaccttcagt
     5401 cggatgttgt cgcgacagaa accaggcttg gcttcgccgt atggcatcat cgatggcgtc
     5461 gagttggctg cctgcctgag atgaaaggcg gcgtgacgga gcatcggcaa cgacagctga
     5521 cttgtctgac accagccgga gggagggccc ggcaaggggg tcggcggtaa agcggtctgg
     5581 aaccgaaatg aaaaagctat cggaaaggcg gtcgaggata gttcgaatca gattcatcgg
     5641 cattgaacgg acacaccggc gctgtgaatg caggtgatct gcggtgccac tgacctcgcg
     5701 acaagagaag tcctggcgcc atcgctgtgg gactagtccg tggggatata acagacggtg
     5761 tggtcgaagt caagcaaggg catgcggaag tcctctgaac tacgggggtt tttcgaccat
     5821 acgcccgatg tcatgagacg gtttgaagtt cgtttaagcg cacggaaacc aatttcgaga
     5881 tacccggctc ctccatcgtc acgccaaaga gcgaatccgc aatacccatc gtccgcttat
     5941 tgtgagtgat caccatgaac tgggcgcctt cagccagctc acacaggacg ctggtaaacc
     6001 ggccaatgtt ctcctcatcg agtggagcat caatttcgtc caggatacag aagggggtcg
     6061 gacggatcaa aaagctcgca aacagcagcg ccatagccgt cagcgtcttt tctccgccgg
     6121 acagcatggt gatgctcttc aagcgtttgc cgggaggctg tgcgacaatc tccaccccgg
     6181 gttcctgcgg acctgggtgt tccgcctgtg catccgcttc aacatccact aactgcagct
     6241 ccgccctgcc gcccctgaag aacttcgcga agacctcgct aaatttttcc tgcaactcgg
     6301 caaacgtcgt cgcgaacatg tccttcgtcg tgcgattgat gcgctgaata atctccttca
     6361 gcgatgcgat cgaattcgac agatcctgct cctgcgtgga aagaaatcga tggcgctcct
     6421 ccaactcctg atgttcattg attgccgcga gattgatcgg ccccatccgg tcaagccgtt
     6481 cgcggatctt cagcaattgc tgacggatct catcttcact tggcgagacc tgtgcttggg
     6541 cctccggctc gagaagagca ggcgcgccgg attcacacac cgatcccgga tccatctgat
     6601 aggtgccgga aagcgtggac tccacagtcg ccaactgggt ccgcacttcg gcccgcctga
     6661 cttcaaacgt gagacgggat tcctgcaacc cggatacgag cttgcggacc tcatccaacc
     6721 tcgtttcaat ggaatgggcc tgggccatgt ctcgcgcctg cccttcttgc gcttccacga
     6781 ttcgtgcctt caattgctcc gctgactcgc cgatctcccg gcacatggct tcctgccggt
     6841 cccgttctgc acggctctgc tcagccgacg ccatcagcaa ttcaagttgc tggtccaagc
     6901 tgccgatacg gacagcagtg tcctgttgct cctggcgaac gcgctgcaaa tcagcttgac
     6961 gatgctcgca ggtattgcga gccccttgca cgctcaattg agcctcggtc agtcgctgct
     7021 ggagaccgtg gatggcctga tcgaggtgca ccacccgctc acggacggct tgcaaggccc
     7081 cctcctgccc gttcttctcg gcgatccact gcgccagctg ggcttcagcc gaggtggctt
     7141 cgccttccaa gcgtcgtacc tccaatgagc cgcgttgcgc atcgcccaac aaggtctcga
     7201 tacgctgagt gagatctccc aacagctgtt gaattcctga cgcatccttt tggagagaga
     7261 gggcattcat ttcttcttca cgtaacgcat cggccacgcg ccgcccctcc tcccgaagga
     7321 agtgactttc cgcctgcaac gactcccgac gctgccgggc ctgctcgacc tctgccacca
     7381 gcgtggttcg cctcacttca agctccatca cttcccgccg gcgttgcaac agaccgccgc
     7441 ttgagctcac ctgaccgccc gtgacgacac cggctgcgtc cacgagttcc ccggcgcgcg
     7501 tgacgaatgt cgcgccatcg gcgcctgtcc tctcctgctc gtgccacaag gccatcgcat
     7561 cctcgagcgt ctcgacgaac accacgcgat cgaacagata atccctggcg gcgttgcgag
     7621 atccgctgat gccgaccaag tcgaccgctc gtccgatgac ccccgctcgg ccgtccatgc
     7681 cggtccacca atcgtgggac cgctcggggg ccatccaccg aggggcctga gggataaagg
     7741 tgccgcggcc gaggtccttt tccatcagaa aggcaatcgc ctgcgatgcc gactgcggac
     7801 catcgacgaa ccagcctctc acgcgctcac cgagcactgc ttccacggct cgatctaatc
     7861 cggccgggac aatcaaccat tccgcgaccg cctcatggac cccctgacag gtcttgagtg
     7921 cagtggcttc ctcattcccc gaccggccat agcccatatc ttcccgcaag acaccttgca
     7981 gagcccgcag atgcgaatcg acggccgcta actcttccga tcctgccgat acctgctgtt
     8041 caacttcaac caacttcact cccgtcgcca cggattgctg ttccagctcg tgatgctgtc
     8101 gccgaagatc caccaccagc ccatcggacc cgcgacacgc ctcagtgagt ctggactgtt
     8161 tctccagggc cgtgtcgtgc tgagattgaa cctcttcacg ctcccgcgtc aggcgtccgt
     8221 cgcgctcgga tatctccctg agacgcgctg tcaactgctc aaggttctgt tcggtattgg
     8281 ctaccagcac cgtcaggttc aacacgtccg cccgtccacg gtcctcctcc tgcagggacg
     8341 ccactcgctg atggaccagg cctttcatct catgatcgag ttcggcgagc gcgtgctccc
     8401 gctcgcggca gacctcttcg agtcgagtca attcggcgca caacgccgct tccgcttcgc
     8461 tcgccgctgt ctgctcgctc aagagacgtg cacgatcctc ggcgccctgt tggcgttgct
     8521 gttcatagag ctcgcccttg tgccgttcga cttccgcagc cgtcaacgcc tgggcctgtc
     8581 gctgttcgat gtgggagagc tcttcccgtc gttggacgat gacgagtccg gtatcggcga
     8641 ttgcgagctt gagaccttcc agttccgcgg acaaacgggc ctgaagagcc gcctgttcag
     8701 actcctgtcc gctgagcgtg gcgagttcgc gctcgatctc tccgatggtt tgccgcaagc
     8761 ggcgcaattc attggtcagc aattgcacct cgagcgtccg ggcttcctgc tgaagcacct
     8821 ggtaggagcg tgcttgccgg gcctggcgtt caagcgagtt caattgcttt ttgacttcgg
     8881 caatgatgtc gcgaacacgc agcaggttct gctgagtagc gtccaatttt cgtagggcct
     8941 ccgccttctg tttcttgtaa cgaacgatcc cggcggtttc ttcaatcagt tcccgccggt
     9001 cctgcggcga ggcattgaga atctggtcga tctgcccttg ggcgatgacg gtgtgtcctt
     9061 tcgtccctgc ccgggtatcc aacaggatac tgcgaatgtc cttcaagcgg cactgcgtct
     9121 tgttgatcaa gtattcgctg tctccgttcc gatagagccg gcgggtgatc atgaggtcct
     9181 ggtattccga caactggccc ggcagcccgt tctgactgtc cactttgact tgatccaggc
     9241 cgctgatgac cagagatacc tcggccatgc ctagcggctt gcgcaattcc gtcccgttga
     9301 agatgacgtc ttccatcttt tcgctgcgca acgtcttggt gctttgttct ccgagtaccc
     9361 agaggatggc gtccacgacg ttgctcttgc cgctgccatt tgggccgacg atggccgtca
     9421 cgccgttggg aaattcgaga cgtgcttcgg cgaacgactt aaaccccacc atctcaagcg
     9481 acttgaggta catgccctgc tcctttggta gttggtcgga ggataaccgg acgagggaaa
     9541 accacgggtg ggttttgtag cacaggactc gaaggaaatc aaagaaaaat accgcaggta
     9601 gtggctggag gcccgagtgc ctaccatact tgggacgagg gaagagtcgc gaggttatct
     9661 ggacgcggcc acggacttgc tggcgggagc gatcgtgatc aattccctag gaaggagaaa
     9721 ctcgacatcc tcttcgatga cagtcaaatc ctcgacttcg gcgctgtcat aggttttgag
     9781 gtaggcgacc acttcttcca cgagcacttc cggcgccgag gcgccggcag tgatgccgat
     9841 cgccttggca ttcttcaccc actcgggatt gatgtcggcg gctgaatcga tcaagtacga
     9901 actaatcccg caatgctcac cgagctcgcg caagcggttc gaattcgagc tgttgggtga
     9961 accgatgacg agaatcacat cgacgagctt cgacaactcc ttcacggcgt tctggcgatt
    10021 ctgcgtcgcg tagcaaatat cttcctggtg cggtcctttg atatgaggga accgtttgtg
    10081 taaggctccc acgatatccc ggcactcatc cacactcagc gtcgtctggg tgacatagga
    10141 caagttggtc gtgttgtgca cttgcagcct ctcgacgtct tcgaccgacg acacgagatg
    10201 aaacttatcc ggcacttgtc ccaacgtacc gatcacctcg ggatggccgg catgaccgat
    10261 cagaatgaga tcgtaccctt gggtataatc ccggttcact tcgttatgaa ctttgatgac
    10321 cagcggacag gtggcgtcaa tgacatgtaa ccggcggcga ttggcttcgt cccacacaga
    10381 cttcgcgacg ccgtgcgcgc taaagatcac gacggacccc tccggaactt cgttcagctc
    10441 ttcgacgaag acggcgccct tgtggcgcaa cgaattgacg acgtgacgac tgtggacaat
    10501 ttcatggcgg acgtagatcg gcgctccgta ctttttcagc gagagatcga cgatatcgat
    10561 cgcccggtcg acccccgcgc aaaacccgcg tggattggcg agatagatct tcatgaccgc
    10621 cctttccttg cccgggacat ccactcacct tacgtgagtt catgagggtc cgtcaacaga
    10681 atagccgccc aaccgctgga gatcaggggg cacgatggct ccggcgttag agggcaaggc
    10741 aattcgtcac cggcggtcat gcacgcgcgc tgaagacaaa gagagggtct gtcaaccccc
    10801 gggcgcttga cagcgactcc cctgccccgt acgctggtcg caccttccat ggccagtgtc
    10861 cccgccaaga aaatcctgat cattgaagat gagcgcgata ttctccagct ggtgaagctg
    10921 tatttggaga aagagggctt ccgaacggtg acagccagca ccggggcgga aggtctcaat
    10981 agcgcaaagc agagcaaacc ggatttgatc gtcctcgatt tgatgctgcc agaaatcgat
    11041 ggtctcgagg tctgtaaacg cctacgttcc gctccggaga cggcgatgct gccgatcatc
    11101 atgttgacgg caaaggccga ggaatctgat caggtcatcg gactcgaact cggcgccgac
    11161 gattatgtca ccaagccgtt cagtcctaaa gcactcgtgg cgcgcgtcaa agcgctgttt
    11221 cgccgcacgg atcgcagccg gcaagaaagc cagaccgcct accaatacgg caagctggcc
    11281 atggaccttg atcgccacga ggtcacggta cagggaaagg atgtccccct tacggccaag
    11341 gaattcggac ttcttgaaca tctgctgcgt catcccgggc gagtcctgac gagagaagtg
    11401 ctgctcaatc aggtctgggg ctacgattat ttcggcacga ccagaacggt cgacgttcat
    11461 gttcgccggc tgaagcaaaa gatcccttta ctcgacgagg cgatcctgtc ggtgaaatca
    11521 ctggggtaca aactcaaggt cctggactga gaatcgtcga cagcgctgca cggcctgtat
    11581 gagtctttcc atccgctgga aggtcaccct tgccattctg ttcgccttac tctgcgggtt
    11641 tgtcatcgcc ggagtcctcg cagctcgatc gctggcagaa caagaactgg ctcaatccgg
    11701 cgaggccctt caagtgtcca cccacctcat tgcgctcgat ctgcgtccgc tcgtcacggc
    11761 atcctccctt ccgccaaccc ctcagcttca gacgatcgtt ggggagttga gccagcgcgc
    11821 gcgtgcacgc gtcacgctca tcgatgcttc cggacgagtg ctcgcggaca gtgccgtccg
    11881 ggacggcgat ttggcgggcg ttgaaaatca ccgcacgagg cccgaaatcg aacaagctgt
    11941 cgcgaccggt tcgggcaccg atcttcgggc aagccacacc accggcgaac gcacactgta
    12001 tcgggccgtt cgcctcggcg atcccggtgc agcatctgct ccgctctatt tgcggctggg
    12061 gttgccgatg accgccttgg aacatgaact gacgattctt caacggaatc tgacgttggc
    12121 cttcggcagc gcctttttga ttgcagttgc aatgagcgtc tggttagcaa gaagcctgac
    12181 caggccgctg tccgatatca cggccgctgc acgccagctc gcagggggca atctgtcagt
    12241 cgccgtgcga acatcttcgc gggacgaagt cgggctgtta gcagacacgc tcaaccgcat
    12301 gacccgcgaa ttgcggtcca aaatcgagga actgtccgaa gaccgttccc agctgctggc
    12361 catgctcacg tcgatggtgg aaggcgtcat ggtcctggac tccaagggcc gtatccttca
    12421 agtgaatccg gcgtgggagc gcatgttcgg ggtgacgcgt aatgaggcgc gcggacgcca
    12481 atcttcggag gtgttcggcc atcccaaatt gaacgccctc gtttcggcgg tactcgcggc
    12541 tcgcaccggc caggaaggcg aaatccttct cccgagtggg cgctgcctca atgtggaagc
    12601 gtcggtcgca gggggagaac aggacaatga agcctgcgcc gtcctcgtct tccacgacat
    12661 caccgaactc cgccgtctgg aacatatcag aaaagacttc gtcgccaatg tgtcgcatga
    12721 actgcgcacg ccgctcacgt cgatcaaggg ctatgttgag gcgctcttgg acggtgggaa
    12781 ggatgacccc gaaacgagcg tacgtttcct cgacatcatt ttgaagcaaa gcgaccggct
    12841 caatttgatt ctcgaagact tgcttcagct gtccaagatc gaatctgggc aggtgcaatt
    12901 caagcaggaa ccgctgcata tcgggtctgt gatcgagcgt accatcgcga tgatcaagcc
    12961 gttagccgac aagaaacagc accggctcat cgcacaagtc gacgccgatt tacccctcat
    13021 caccggcgac caggaacggt tggttcaggt gctggccaat ctgctcgaca attcgatcaa
    13081 atacacgccg gagggcggcc aaatcacgct gtccgcgcga cgccttcccg cgacgcggac
    13141 cggacctgga gacggcgtcg agctcatggt gagtgatacc ggtatcggca ttcccgaagc
    13201 ggatcgcccg cgcgtgttcg aacgatttta ccgcgtggac aaggcgcggt cacgggaact
    13261 gggaggaacc ggcctgggac tggccatcgt caagcacatc gtcgagggtc acggagggca
    13321 gattcacgtg gaagcgaatg ccccatccgg cagccgcttt gtcgtccgcc taccggctta
    13381 acgccctgca tcgatctcta caggaatagg cccatgatca gatagatcgc ggtcgagatg
    13441 atggcggcgc caggaagggt aaacagccag gcatacagga ttcgcctcgt cactccccac
    13501 cgcactgctg acagccgttt gatcgcgccg acccccatga cggatgacgt gatcgtatgg
    13561 gtcgtgctca ccggcaaacc gatatgggcg gtgaacatca agactgtggc cgcggcggtc
    13621 tcggcggcaa agccgtgcac aggctcgagc ttcacgatct tcattcccaa ggtgcgcacg
    13681 atgcgccatc cgccgacagc ggttcccaat cccatggcca ccgcacaagc cccgatcacc
    13741 cacgtcggca cttctgctga tggaatttga ccggcggaga gtaatgccaa tgtaatgatc
    13801 cccatggact tttgcgcgtc attcgcccca tggctgaagg ccatgaaaca cgcggaaatc
    13861 agctgcagtc gcttaaaaag gcgggtggcg accgcccggg tgacgcgaaa gaacagccag
    13921 ctgatcagca ccattaaggt gaggcccatc gcgaagccga aaaacggcga gagcaccatg
    13981 gcttcgagca ccgatcgcag gccggcaact ttgaccgtat cccacccgcc gtgcgtcagg
    14041 gccgctccca ccagtccacc gatcagcgca tgcgacgaac tggtcggcaa acccaacaac
    14101 agcgtaaaca ggttccagaa aattgcgccc gctagggccg cggccaccat atgctgtgtc
    14161 acgccggcag gatcgacgat tcccgagccc accattttcg ctaccgccgt ggacatgaag
    14221 gccccggcaa tgttgagcac acccgccccg atcaccgcga cgaacggact gatcacgcgc
    14281 gtagagacga ccgtagcaat cgcgttggcg ctgtcgtgcc atccgttcga aaaatcgaac
    14341 agcagagcga gcaccacgac caggagcagc ataccggtca gctcaggcat cgagagcctg
    14401 tgaggggtgt ggagcgggaa aggagaaaca cggcatcgag gggaatccag ccatggcctt
    14461 gtgacttctt cgcattccta tcacggcgcg agacgtgaga atggctccat cctgctaatg
    14521 gtgcttcagg gcgatgcgtt cgagaatgtt ggcgacgtcc tcgcaccggt cggtacccgc
    14581 ttcaaaccct tcgtagattt ccttccactt gatgaccgcc acaggatcgg tctctttctc
    14641 aaacaattcc gaaatcgcat cgcgggagat ccggtcggcc tcgttttcga gactgttcac
    14701 ctggacgctg cactctttga tatccggatg cgacaggccg agcctgtcca cgcccgcgcc
    14761 aacggccact gatgcctgat acaagacgtc agccagtttg acagccttat ccgtgggtgt
    14821 cgtcactttg tacaacacga agcgatcggc aatcgcttct gccacgtcca agatatcgtc
    14881 caatgcgctg gccaaatcat gaatgtcttc tcgatcgatc ggcgtgatga atgtttgatt
    14941 gagccggagc gcgatgtcat gcgtgatgcc gtctcccacg tgctcgacat ccttgatacg
    15001 cttggcctga tcgacaggag attcaaaatg ttccatcatg tctttcaaca gccggctgcc
    15061 ttctatcatg ttgtgggcgg cgttcttgaa taactcaaaa aaccgttcct ctttcggaat
    15121 caagccaaac atgatcatcc ctttcttgcg aatgtcctct cgctcggcaa cactctcgca
    15181 gcggtgttac gtcacgatga cagaggcgtt acgggactgt aaccatttct caccaccgga
    15241 tgagacccga cgcccacgtc aacccgcccc cgaagctgcc aagtgcaatc aggtcatgcg
    15301 gtcggaccct tccgctgcgc acataatggt caagcgcgat cggcagggaa gctgatgagg
    15361 tgttcccgta tcgctcgatg acggagcaca gggaatccgg cgcaatcgcc aagcggctgg
    15421 caagttgggc aaggattctt ccattggcct ggtgcatgat gaattgcgac acatccccct
    15481 tgtcgacgcc gaactctttc aagatgtcga gcaagccccg ttcgagatgc ctcaccgcca
    15541 atcggaacaa cggcgccccc tgcatccgca aggagtgctc ctggccatcg agagtttcgt
    15601 gacttgccgg tctccgcgat cccccggcac gaatgcgaat caaatcatgt cgtgcgccat
    15661 cggaatggag ccgaatcccc agaagtccac ggctcggctg atcggcgtcc ggttcaccat
    15721 gcaccaccgc tgcaccggcg ccgtccccaa agagcagcgc ggtgtcggag tcgcttgggt
    15781 cgagcgtgcg cgacttcacc tccgctgcca cgacgaggca ggtcttcgcc tggccgcttc
    15841 gaaccatcgc atcggccatg gaaagaccat acaaaaaccc ggaacacgat gcggcgacgt
    15901 caaatgccgg cacgctggca caacctagct tccgttgcag gtggcaggcc gtagaaggga
    15961 aggccatatc cggagaggtg gtcgaaacga taatgacctc gactgccccc aacggaagcc
    16021 ctgctgcctg cagggcgtcg cgggcggcgg acacagccaa atcggaggaa gcctgggacg
    16081 cctccgccca tcggcgctct cgaatcccgg tcagttgcat gaccttctca ggatcaattc
    16141 ctaacgccgc acccgcctcg cgattggaga cgacgcgttc gggaagatag gagcctgttc
    16201 cgatgagcct ggatcgaatc attgctgaat tgccgagggg tggcaccaac cgcgcgggat
    16261 tataggtctc gcgcccgcag gggtcaaccc gagcgggaaa gattgctttt gaaaggggct
    16321 cctgctagtt ttgtaacagg acctcatgaa gatcaatctg gtacagacag accttctccc
    16381 ggccgcccgt agcctgagcg ttgccctagg tatggcggcc ctctgcctgc tcatggcctg
    16441 ctcgagctcg ccaaagaacg gcgatgccac gtccaagaaa gctctgagtg gaacggacga
    16501 atcgatcttc ctcggcgata cgattgaaaa gaactacgat cccaacgtca ttatgaagcg
    16561 cggagaagcg ttcttcgaaa aggaagaatt cgccgaggcc atcgtcgaat ataaccactt
    16621 cctcgatctg catcgcagtc acatgctcgc tccctacgcc ggtttccgga tcggcgaaag
    16681 ccacatgaag cgtgcgaagg gcattgatcg cgacccggaa cccattcaaa aagccattga
    16741 ttccttcgaa cggttgcgaa gagactatcc gggcagccgg tacgacagcg aggcggtcga
    16801 caggattcag gaatgccatg atctgttggc tcagatgcac ctctttgtcg ggcagtttta
    16861 ttaccggcgg cagtcctatc tggccgcggc acaccgcttc gagcagatca tgaagattta
    16921 tccggacaag tctgtggcgc ctgatgcgct gtacttcctc gctctcagct accacgacct
    16981 tggagcggac gattgggcga gcgagaaatt gaccctgctt gctgaaaagt atcccaacag
    17041 tacgcattcc aatgacggcg caaaattgct tgccaaaatc atcaaatccc agccgtcggt
    17101 gctcgtggcg aaaaagccgg aggcagctgc cccgatcccg gaaccggccc ttgcggctca
    17161 agagagttcg cggtccgttc cggcaggggg cgtgactccg caggtggcca atggactgat
    17221 gcggcttcct tctgccacgg ccatggggca ggggtttgtg aactgccgcc tcggcgcctg
    17281 gtgctagcag gctgatcaaa cagaccacgt ttctccccca cccgtcccgg tgcgccgaga
    17341 gacgcatcat tcaccgtagc gttctcgctt cgctcaatcc ctcgacagac caaacaacgt
    17401 atgcgtcgct ctttcgcttg ttgcctttca gggtgacggg cgcgtctccg tgcgcctcgg
    17461 aactggtgaa catgcctgtt tgaccagcct gtcgaagaag aagaagaaga tcaaatgaag
    17521 aaatggggga agagtctgtg aaagctactg gcccagatac cagccgatgc catgccgctc
    17581 caaaaaactc gtaatcatcg tgagcgaatt ggcatagatc atcacgccga cgatgacaag
    17641 caataccccg ctgacagtgg acactcccca caggtaggcg cgggcttgct tgaaatacgc
    17701 cagaaaacga tcgacaccca aggccgtcaa gaacagcggc aagcccaaac caagcgaata
    17761 actggtcaac aagaacacgc cgtcgagcaa ggaatcggtg gtgctggcat acagtaagat
    17821 tgtgccgagt acgggcccga cacagggcgt ccagccggct gcaaacgcga ttccaattaa
    17881 aaacgaaccg agatatccca ccggccggtt ccggaattgg aagcggtgtt ccatctgcaa
    17941 aaaattaatg ttgagaatgc cgagcagata gaggccgaaa atgacgatca gcagaccccc
    18001 aatacgccgg atgtgctctt gataggtcat gaggagctga ccgacgaaac tggcggaggc
    18061 cccgaaggca atgaagacga cggaaaagcc ggcaatgaac aacagagcat tcaccacaat
    18121 cgctctcttg aactgtgacc gcgcattggc atcggtcagc tgctcgattg ataaacctgt
    18181 gatgtaggag atataggagg gaacaagcgg cagcacgcac ggtgagacaa acgacagtaa
    18241 tcccgccgaa aatgccgcaa tcatcgatat ctgagtcagc gattcggaca cgatcaatct
    18301 ctatgatttc atcaacatct cgatcaattg gtgcgcttcg ggagcttccc agtcacgggc
    18361 gccgaagatg tgctgccgca ccacgccctg acggtcgacc atgaatgtca tcggcagcgt
    18421 ccgtgcccca tacgacaacc cgacgcgaaa atcggcgtcg tgaagaatcg gaaaagtgag
    18481 tttgttctct tcctggaacg gcctcgtgac cgccactccc tgtgcatcgg tcgacacggc
    18541 gagaatctcg aaatcttttc gatcgtacgt ccggtacaga cgttccatgg ccggcatctc
    18601 tacccggcat ggcccgcacc acgtggccca gaaattgaga agaaccactt tcccgcgcaa
    18661 ttcagacaga gagacaattc gcccgttcat gtcgcgaagc tgaaagttgg gggccggctc
    18721 gccgggcttg accattccgc gttgcgccac tgatgccgcg ctgccggcct ggctcgaggc
    18781 cgacaaggcc atcgtcagga atacggagag gagtgcggca gtcacaagcg gtttcatcct
    18841 tgatccacca tcgaagcttc tgcaagtggt ggtcgagttc cagccgtctt tggcgtgacg
    18901 tttagcagct tcgtcaatat ctggaggctg tcgagcctcg tccaatcacg cggtccgatg
    18961 acgatttctt gaatgatgcc ttgctgatcg atgatgaacg tctcggggac gcccatccgc
    19021 ttatagggtt tgtcggtccg cccccaggaa tcgaccaaga ccgggaacgt cagatttaag
    19081 cccttgacga acggaggaat atcctttgtc gttgtgaccc ggtcgatact gacggctagc
    19141 atcaccaacc cgtctttttc gaaattcttg ttcaggatct ccatcgaagg catttcttcg
    19201 cggcagggct tgcaccaggt cgcccagaag ttcaagaaga caaccttccc tcggaaatcg
    19261 gacagacgat actgtttgtc gttcagatcg gtgagcgaaa aatcaggggc cggcttcccg
    19321 acggcgagcg gctcatactt cgcgctttgc agccaaacca gggtgaagac ggcgatgaga
    19381 atagccgtgc caacggccaa aatcagcacg cgtgagcggc ctcgagatgg aagtgaagtc
    19441 acattcgaag ggatggtttg ctcggtcatg gtgacctgaa taacgagagg tcacatgcat
    19501 caggcatagg catgcagacc ggacaacaga aaattcactc cccaaaagca aaaaatgacc
    19561 atcaagaagc cgaacacggc gtagatcgcg gcacgccggc cttcccagcc tctcgtcatc
    19621 cgagcatgga tataggcgcc gtagatgagc cagacaatca gcgaccaggt ttctttcggg
    19681 tcccagctcc aatagccgcc ccaggcgtaa ttcgcccaca tagcgcctaa aataatcccg
    19741 aaggctagca tgggaaatcc gatcatgatg gccttatagc ccaactcatc tatggtttcg
    19801 aggtccggga aggccgcata aaaacgcgac cggcttccgc gctgctcggc ccgttccttg
    19861 aacagataca tgactccaag accaccggcc atcgcaaagg ccgcatagct ggtcagagtc
    19921 acggagacgt gaatgtagat ccaataacta ttgagcgccg gcaccagagg ttcagccgtc
    19981 tggtagcgat agggaaggag ggacgctgct cccatcgcga tgaagccgat gccgacgacg
    20041 aacgcgccga tcgccttgat cttatatcga agttccaaca gcacgtaccc gagaatgatc
    20101 gcccaggaca cgtaggccat cgcttcaaac tggttggacc agggggcgaa cgtgcctgaa
    20161 tgctctaacc gctcgaacgc gcgggtcagc aacgccgcag tgttcaccat ccacccgaac
    20221 accgtcacca gcgtggccac ctgccctagt tgtgcggccc acgctccgtc ccgttcatcg
    20281 aagtcctcgg ccggatggcc ggccggtgcg aaagccattg cgggccggcg cgcaaataag
    20341 taggcgacat acagggtcag ggaagccaga tacagccaga aggtcatgtc gaacagaaac
    20401 agtgaacggg ccatcgtatc ctccctcaat ccgcgggttc agtcgaacct gtctatcata
    20461 cccaagagca gtggtctttg accactggta ggccgctttc ctcttccgtg atcgcctgcc
    20521 cttctgtcag ggaactccag tgggcgcact tcaaagaagc cccagccgga aggcgccgcc
    20581 cggttaggct tcaagtgtac gtattttttc cgtgagcttc cgaaactctt tttgaaaatc
    20641 gatctgactc ttgtgcgtcg tgccgccgac gtgcacggtc acaccgctgg cccctgaggg
    20701 gatgatcttc gtccacaacc ggcgatggta gatgaaggag gaaagggtga ttccgacgac
    20761 gatcatcgtg cacccgatcc acacaatgtt gatgccgggg ttcttggcaa tttgcagacc
    20821 tgtgtacttt ttcggcttgt agccgatcaa ttcaaattgg tatttcgagt ctttgatctc
    20881 gaacaaatcg ggaaattggt agaagatcca gggagtggcc tgaacggtgc tgcgctcatt
    20941 gaccgtcaac ttgatcgccg ggttggcatg ctcaatggtt ttggaataga cctttttttc
    21001 ggttgaattg aaggcaaagt ctgcaacaaa gtctgtcatg gccagcttca agttcaaatc
    21061 cgagagcgtc tgctccttct gccattccaa gtcgatggtc ttgacgactt tctcggtctg
    21121 tttgtctttg atgttcaccc gcgccacttc aatttggtcc caggcatctc cgtagctgga
    21181 ttgataaaac cagataccct tatacacaag agggtcgttg acggtaatcg tcttggtgat
    21241 tttcgtttcg ccgctctcga gcacggtgag cgtactgtta taggacttga ccgcgccgtt
    21301 ctcgtggtaa tcaatccaga acttgtccac tttcaaatcg aaattgccac gaggaatatg
    21361 ataggtctgg ccttccagac agacgccaaa ttcctggaac ccataatagc ttccgagtag
    21421 tccgccgagc acgatcaccg tggcgctcaa atgcgccatg tgcgcgccaa cgcgccccat
    21481 gactcctttc gtcgcgtaca gcgtcacttc ccccgcctca ttcttggcca ggacccgata
    21541 gcccttcccg acgtagtgct gggccaactg ctgtgcgatt gattctttgg tgccctgcac
    21601 cacgacttcg gcctgctgtt tcatcccttt aatgaaagcg agtgagacgc tgaccttgtc
    21661 ttgtttcatg gaccgccaga cggccgggaa acgcttgtag aaacaggtga gcgagttgac
    21721 acacagcagc ccgagcagtc ccgtaaacca ccacgtgtgg tacacgtccg tgaagccaag
    21781 acgaagaaac cagcgatagg cgtcttctcc atattcctgg atgtaggtct cagcccgctc
    21841 gccctgctga atgaccgtcc cgatggtcgc cgtgatggcg atgaacagaa acagaaacat
    21901 cgccaactta atggaggcga agaactcaac tagctcgcgc gaaaattctt cccatcccac
    21961 accgggggcg ggagtttggg gggtatcctg ttcagacaac gtttccggcc ggctgttcat
    22021 actggtgttg gtctctcttc catagccaga cacacgatct ctacggcccg agaaacacgg
    22081 cagatggggc gaggggcaat cctgcgctgg taggcgatcc ttatcccgac aacgcgtgta
    22141 ccttgccgta gatcaggaag gaaatcctcg aaagcacagc atcttacaag cgccggaaaa
    22201 gcactgtcaa gcaagcgcgt cgatcgccgt gtggtcatac cccactatcg aactaataga
    22261 caaatcaagc gattatcgct atgatctccc ccggcttcca catcaaggct tccgtctcac
    22321 acagggcccg ttgtaccccc gactgagatc gagcaaccat cgcccgctga accatttgag
    22381 gagtggcaat gagaaacaat tacctcttta cgtccgaatc ggtgaccgaa gggcatccgg
    22441 ataaaatcgc cgaccagatt tccgacggaa ttctggacgc catgatcgca caagacaaat
    22501 acagccgcgt agcctgcgag accatattga ccaccggcat cgcattcgtc gccggtgaga
    22561 tttcgaccaa agcgtacgtc gaaatcccgg acattatcag agatgtcatc aaggatgtcg
    22621 gctattgcga cgcctcctgg gggttcgact gcaacacttg ctccgttctc acctccatcc
    22681 atcaacagtc cggcgatatc gccatgggag tagattccgg cggggcaggt gaccaggggc
    22741 tgatgttcgg ctacgccacc aacgaaacag cagagctcat gccgatgccg atcgtgctcg
    22801 cgcaccgact cacccgccgg ctagctgaag tgcggaagaa gggaattctt ccctgggtcc
    22861 gtcccgacgg gaaatctcag gtcaccgtcg aataccgcaa cgggaagccg attcgcatcg
    22921 ataccatcgt cgtctcgagc cagcacagcc ccgacatcac gagtaagcaa attgagcgta
    22981 gcatcatgga aaaggtcatc aagccggtca tgcccaaagg gctgtacgat ccgacgagcg
    23041 tgaagcacca catcaatccg acgggtcgtt ttgtcgttgg cggtccgatg ggtgatacgg
    23101 gcctcacggg tcgcaagatc atcgtcgata cctatggtgg ccatggcagc catggcggcg
    23161 gcgccttctc cggcaaggat cccacaaagg tggaccgttc tgcgtcgtac atggcccgtt
    23221 acattgccaa aaacatcgtg gccgccggcc tggcggacaa gtgtgaagta caactggcgt
    23281 acgcgattgg ggtggccgat ccagtctccg tcctcgtgga caccaagaat accgagaaag
    23341 tctcggtcga caacctggat aaactggtgc gcaagcactt cccgatgacg ccgcgtggca
    23401 tcatcgatca tctcaaactc cgccggccga tcttcaaaaa gactgccgcg tacggccatt
    23461 tcggccgcaa tgaaccggaa ttcacctggg agaagaccga caaagcgaag gtcttgcgaa
    23521 aggacgcggg actctgatcg cattcggcaa cggttgatcc gaagatcaag gggacggatc
    23581 gagcccgtcc ccttttttct gacaacttga ccatccatta cccgctcatc actgcaactg
    23641 gaggcttttt gtggattacg atgtgagaga catcaagctg gccgaccaag gaagactcaa
    23701 gatcgaatgg gccgaagcga ccatgccggt gctgcggctc attcgaaaac ggttcaaacg
    23761 gcaacagccc ctcaagggcg tccgggtcac ggcctgcttg cacgtgacga ccgaaaccgc
    23821 caatctggcc atcacgttga aagccggcgg ggctgatgtc cggctttgcg cctcaaatcc
    23881 gctcagtacc caggatgacg tggctgccgc cctcgtccaa catgaaggca tcccgacatt
    23941 tgccatcaag ggggaggaca atgccaccta ctatcgtcac atcgagtcgg caattgccca
    24001 tcgcccgcat gtgtccatgg acgacggcgc agacgtcgtt tcgcacctcc attccaagcg
    24061 aaaagatctg ctgaagaacg tcatcggggg cacggaggaa acaaccaccg gtgtgatccg
    24121 gcttcggagc atggcggaca agaaagtgct cagattcccc gtcatctccg tcaatgatgc
    24181 cgacaccaag cacatgttcg acaaccggta cggcactggc cagtcgacga tggacgggat
    24241 tgttcgggcc accaatcgcc tcgtgtgcgg ttcggtcgtt gtcgtcgtag gctacggctg
    24301 gtgcggacgc ggcattgcca tgcgcgcgaa aggcatgggg gctgatgtca tcgtcacgga
    24361 aatcgacccg ttgaaaggcc ttgaggccgt catggacggc ttccgggtca tgcccatgga
    24421 gttggccgct ccgatcggcg atttcttcgt gactgttaca ggcaacattc atgtcatcag
    24481 aagcgaacat ttcgccgtca tgaaagacgg agccattgtc tgcaacagcg gacatttcaa
    24541 cgtggaactc gacattccga gccttgagaa gcaggcgaaa aagcgccggg tggtcaggcc
    24601 tggcgtggaa gaatttaccc ttcggaaaaa tggccatcga gtcagcctgc tgggcgaagg
    24661 ccggctggtt aatttggcta cagccgaagg ccatccctcc agcgtgatgg acatgagctt
    24721 tgccaatcag gcgctcggcg ccgaatacat cgtcaagaac tacaagcacc tcgagaaaaa
    24781 ggtctatccg gttcctccgg cgatcgacaa agaaattgcg cggctgaagt tggccggcat
    24841 gggggtagcg atcgatacgt tgacgcctga gcaaaagaaa tatttggcga gttgggagat
    24901 gggaacgtag gatcgcctag ctgaagcgct ctgctcgacc gtgctaagcc tcaggtgctc
    24961 tgcgcgcaaa ctccttcaag ttacgctgag ctcgacctga acttcaaaca tgcgccgcag
    25021 ttctcaacga ttcggaccgc agcctttcgg caagcacggt gacccgttcc tcacgcaaat
    25081 cgccagatag accagatcca tcccaccaaa gcagcggtga ctcatcgccg ctctgaggct
    25141 cgcttcaaga atctccacct cgctccataa cagcgtggcc tccccgcatc ctgagcctca
    25201 tgtcttaccg gtcagcgtga actggaatga tcaccgctga tcctcacaca cgcgtgatgc
    25261 aagaccgcaa ctcttggcgg cacatgcaat caaaatttgt cgtttcgcaa catttttgtt
    25321 catgagccag cagcccatgt tctcgaatgg gctgattaag gcggggtttt ctgcatatgg
    25381 agctcagctg ggttcaggcc taaagtttgc tcgtgacacc caatagacgt gaagtgttcg
    25441 cgaagtctgc gtcgtcgtct cgagaagcgc gcatggataa actcttactg acctgttggc
    25501 gctgctccgg tcttattcat ttctcaatgc atcgtatggc gtcaatgtca tagctggcct
    25561 ctcccacctg atggggcacc atggcggtac gcattaggaa atcgccgatc ggatcccacg
    25621 agactgaaca ccgagtattc gcatcccctc tctccctgac atcggatcgc accacgacac
    25681 gaggccccct ccagccacgc tccgcaatcc ttacgcgacg gtatggtcaa acaacggcgg
    25741 cggctcaagg aaacctgata cgagctggtg tgctgctgct tgtcctgttc atgcctctcc
    25801 tgagcgccta cgccacagag ccagcaccgg ttgatccacg gaagatgacg ttcgatgcgg
    25861 ttggatatct tccctcggaa cctgaccgcc tggtcttccg aaacgggctg gtggtctacc
    25921 ttctcgagga ccatgaacta ccgctgatca cgattggtgc attgttgcgg accggcggtt
    25981 ggcttgatcc cccggacaag gtcggcctcg ccgcattgac cggaaccgtc atgcggactg
    26041 gagggagcgg tggttggtct gcggtagaag tcgaagacga gttggcacag ttcgcaggtc
    26101 gtatgaacat cgctatcggt cggcactccg ggtcggcaac gcttgatgtg ctcaagaaag
    26161 acctgaagcg cggcttacaa ctttttgctg gtgcacttcg gacaccggcc ttcgatcctg
    26221 cgcatgtgga actcgccaaa ctacaagcga tcgagcggat acgcagacgt gaggacgagc
    26281 ctgagtccat cgcgagtcgt gaattcgtca aactgctcta tggtccgagc catcccagtg
    26341 ccagagagag ctcgattgac tccgtgcagc ggatcagcag ggaggatctg atcgccttcc
    26401 acgccaatac gattcatcca aacggcatca ttctaggtgt caccggcgat ttcagaaaag
    26461 acgaaatagt ggcctctctt ctggaggtat ttggagattg ggaagctgga gcggtgccgg
    26521 aagtgaggat tgccgatgtc cctgaaagcg aggcacaaag agcgggtatt cactttatca
    26581 acaaagacac ctcgcagact catcttcgcg tgggggatct gtcgattaag gaaaatgata
    26641 cggattatat ccctctcgcg attgccaacg atattctggg cggtgacgcg ggagtgggcc
    26701 ggctattcag agaagtgcgg acgaagcggg gcctcgccta ctcggttggc agcgagctca
    26761 gtacgggtgt ctacgatcaa gggatgtggc tgctgtgggc agagaccaaa ctgccctcga
    26821 ccaaagaggt acttggacag ttggtggcaa acatcgaacg aatatcttcc gaacttgtca
    26881 gcgatgagga gctcgatgaa tccaaagaag cctacgtgaa ctcttgggtc tttgattttt
    26941 cgagcgcttc gaagatcgtg agccggctta tgaggttgga atatgatggt ctgccaaaag
    27001 atttctttca gcaggttcgg gaaaaggtac tcaaggtctc gaaggatgac gtcttggccg
    27061 ctgcgaagaa acatctccgt ttggatcgcg tgaaaatcat cgcagtggga tcaggcggaa
    27121 cactgtccga agtgctgtcg acattcggtc acgtgaagga aatcaaacgg agttcagaag
    27181 gacggataca tcgtggtaga gtactgctac gcgccgatga cgcggtccgc gagccctctt
    27241 atgagatcga ccttccaccc tctgtcgctc ggtgatttcg ggatctcctc cgaccagggc
    27301 tttcttccgc atgaccctcg agagagtgta cccgactgcc agccgctgaa tgatcttggg
    27361 cacgagttgc caaagttatt gagcactcgc acggtgagac cgttcatcga gaagcagcac
    27421 tcacttcttc cttccattcc tcgttcctgg gaagagcaag actatcgagc agccatgcga
    27481 attctctcgt tcgccggcca tgcgtatgtc tgggaatcac cggatcatcc ggctgcctcc
    27541 ctgccgccgc agctggcaca accctggtat gaggttgcgc agcggctcgg ccgaccaccg
    27601 gtgctctcgt acgcctccta cgcgctggat aattggcgac ggcttgactg cacacgaccg
    27661 atcgagctcg ataacatcgt cctcttgcag aattttctcg gaggcctgga tgaggaatgg
    27721 tttgtcgtgg ttcacattca gatcgagcgg caggcaggtg ccggcctggc cggactgctg
    27781 caggccatca acggtgcagc gtccgatcgg gagcctgagg tgctcatggg cctccgcgcg
    27841 ctcgcctcgt cccaaaccgc catgcgagac acgttgcttc gcatgcaaga acgttgcgat
    27901 ccgtatgtgt actatacgcg cgtgcgacct tacatccacg ggtggaagaa cagtccgtcg
    27961 ttgccggatg gtttgtcgta taccgggctg tcaacctatg ccgggcccca gcaattccga
    28021 ggtgaaaccg gcgcccaaag ttccattgtg ccgtctctcg acgctggtct tggcatccgc
    28081 cacagcccgg atcccttgac tgtgtatctg caggagatgc gcgattatat gcctcccctc
    28141 catcgggcct ttctgcagcg ccttgaacaa gcaaccgacg atctcgaccg gccgctcctt
    28201 gccggctatg tgcaagaccg gaagagcagt cgctccgagt tatgggccgc ctactgtgat
    28261 tgcgtcgatc tgcttgcgca gtttcgcgag attcacattg gttatgctga tagctatatt
    28321 taccgacagc atcaatcaca cgtcagcaac ccgacagccg tcgggaccgg cggcactccg
    28381 tttatggcct accttcagaa gcacttggac gaaacgaagc aagcgacgac agactaacgt
    28441 ctggatttag gttcaagcgg gaacctgcac ggcacctgtt cagctagggt tcttgctgct
    28501 ttccagactt gagagcgagg tcttcgtcca aggcaggaac cgccaaaaca gaatctgcgg
    28561 ccttctga
//
LOCUS       DASSCQ010000047         9527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_709334, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000047 DASSCQ010000000
VERSION     DASSCQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709334"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFH05_03535"
     CDS             complement(<1..363)
                     /locus_tag="VFH05_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET6677313.1"
                     /translation="MTGRPQFPNEQSAKGEFTRQEDRFRGWVSADGASGYPAASGRYH
                     LYVSWACPWAHRTIIVRKLKQLEHVVGMTVVDPIRDERGWAFRNGPGHSQDPINGFRY
                     LSEAYKATDPTYLGRVTVP"
     gene            644..1897
                     /locus_tag="VFH05_03540"
     CDS             644..1897
                     /locus_tag="VFH05_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677314.1"
                     /translation="MAEFNPANSKRLREQADQLSRTTSHQLPKLTPHSVEALVHELRV
                     HQVELELQCQELKDAQAELEESRDRYRELYESIPIGYATIGGSGRIHDLNPAGMSLLG
                     LPSTLSKLPNFMMFFVSEGDSDVVARLCRSALREQQPGSAELRMRKSDGSLFTAALHA
                     EPVLTGAGKGERVRITFKDVTRTREVEESLRQQQLELEANRVELQDLMAKLFTAQEEE
                     RRRIACDLHDDHCQRITAMILEASSIEKLVRVAMPSLLPRMGALKEKLNGILEDFRHL
                     THELHPRHLDTVSLACSMRMHIKEFIDYTDLRVEFKEHDVPAHLPLPITICLYRLLQE
                     SLGNTRKHANAKEIVVELTGRKDEVELSVSDDGAGFNFADYKKGLGLRSMQERVRPLH
                     GRVRIDSQQGRGTTVLINIPLPSGA"
     gene            complement(2072..2434)
                     /locus_tag="VFH05_03545"
     CDS             complement(2072..2434)
                     /locus_tag="VFH05_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6677315.1"
                     /translation="MTGKISRSGFFLLISLALPVPLMAQEYPPDLSRGKAIYERHCLS
                     CHGVDGHGDGPNAVLLTIPPANFHRPGSFLKSDESLLRMIEHGGVYSPMHAWRGRLTD
                     GEMQDVLAYIRILSQDGR"
     gene            2625..3884
                     /gene="pfp"
                     /locus_tag="VFH05_03550"
     CDS             2625..3884
                     /gene="pfp"
                     /locus_tag="VFH05_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET6677316.1"
                     /translation="MSASRPIVGMLVGGGPAPGINSVIGAVTIRSLLGGSEVLGFVDG
                     FKWLMEGSTGHVHPLSIEEVSRIHFRGGSHLGTSRANPTKKPEHLDRVLSTLTSLGIT
                     RLVTIGGDDTAFSALKLEARSAGRMQVVHVPKTIDNDLDLPYGIPTFGFQTARHYGVE
                     ILKNLMVDARTTSRWYLTVTMGRKAGHLALGIGKAAGATLTIIPEEFRERPVKLQRLV
                     DVVIGTMVKRIASGRTDGVIVLAEGLIEIIDQNDLGGLDHVERDEHGHLRLSEVDIGD
                     LLRREVTKGMKALGLSVTVVSKNVGYELRCADPIPYDMEYTRDLGYCAAQYLLDGGTA
                     AMVTIQDGRFAPMPFKEMLDPVTGRARVRMVDTESQSYQIARQYMIRLNDEDFTDQER
                     LSRHAAVVGLSPDAFKQRFQLNRGKRE"
     gene            3902..4759
                     /locus_tag="VFH05_03555"
     CDS             3902..4759
                     /locus_tag="VFH05_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6677317.1"
                     /translation="MQVLVTTDGSKYGKWALEWVGQLPLAEEPTVKVLHVVDIGRLRA
                     PFMLQPAVIGTERYLKAEITKLQTQAERTKHEAARWLKSAGLKGRVVIERGPVAATIV
                     KAAKRGVTLMAVGSRGLDALDRFMLGSVSTHVIHHVSCSVLVVREAPRPIRRIVLAVD
                     GSTASKRALQFLLRAMRPSTGRPSQGPIRVTVTHAMPFLKYPEVQQAGKAVVEACSTK
                     LVRAGYVVEQMPIIGNPADKILKTAETHEADLIVTGAKGLGAVGRFLLGSVSTRVVQH
                     AACSVLVVR"
     gene            4764..5060
                     /locus_tag="VFH05_03560"
     CDS             4764..5060
                     /locus_tag="VFH05_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677318.1"
                     /translation="MERNQVSSPHTSQAGRLGLGLVVVVCLSIVLQMLGVPAPLLNAG
                     ESLDAGEASVSEGFSLLTISSDCILSSTRVLVTYRDSSFHVPILAASLFHPPLI"
     gene            5144..5557
                     /locus_tag="VFH05_03565"
     CDS             5144..5557
                     /locus_tag="VFH05_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677319.1"
                     /translation="MTRIALSVCAVALCAFAACSHGTATSSGRTVRTVEISDHVKPGV
                     MYASPGDEVRWMNARENPVRVGFLNRNLLEDHQCQNGIVNLLGEVNDLITIAPGESIS
                     LCPVRAGDLRYNVWFDADNPRGSMSPTMTVRIRGG"
     gene            complement(5561..7348)
                     /locus_tag="VFH05_03570"
     CDS             complement(5561..7348)
                     /locus_tag="VFH05_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET6677320.1"
                     /translation="MYPYALIGNCHTSALINATGTIEWMCLPRPDSPPVFGRLLDPDG
                     GHFSITSSLSSSPEPTTSQSYLSNTNILTTTVTLSNGDSFRITDFCPRFEQYGRMYRP
                     AALFRLVEPLKGTPAIQVSCRPVCGWEKTAALPVRGSNHLRYDIRGESLRLLTNMPLT
                     YLCEETPVALTRKFYFGLTWGLGIEDDLIKVTHDFLEQTTRYWRLWVKHCSVPLLHQQ
                     EVIRSALALKLHCYEDTGAILAAMTTSLPEQVGGQRNWDYRYCWLRDAYFTLTAFNNL
                     GHFEEMESFLNFLLNIALTHEQSRDRLRPVYTLSQGLPLPEIEHANWSGYQGSAPVRS
                     HNLAADQVQNDAYGEMILTFTPIFFDERFLDLRTRDLDGLLTHLAQLCARSIGRQDAG
                     LWEIRGGWQEHSFTNLLCWAGLERLERIKRAGFLKSITLDLTAERERASRALLRAVRD
                     GAVRNGPDDATHDAALSQLAILGYPDRALCDSTLGQIVRELSLTFDASETGFFYRYVR
                     EDDFGKPEGAFVVCSLWIAQALARLGNLNHARAILDRVVTAGNHVGLLSEHFIPRTKD
                     QCGNFPQAYSHVGLINAAFAVSPPWSDVL"
     gene            7397..>9527
                     /locus_tag="VFH05_03575"
     CDS             7397..>9527
                     /locus_tag="VFH05_03575"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET6677321.1"
                     /translation="MRLSLRFLLPLAVVLAGLAYAVIPLVDSLTLKWFVRDVEIRAEL
                     IGRMVEGPLGDLLASESRTKLPAYFNRIIQDERLFAIGFCDLRNRLLYKTHSYPDAIA
                     CEGNETIRAGETALLQLPRGAVHVSAVGIEGNGGPVGRLMLVHDMSWVQRRSTDTKWY
                     LFYLFAIIGAVISSVTVLVAHLSWRGWVTGVRSMLRGEGLIALIKDKHHGPELQPVAK
                     DLRALIHHLEADKRMKDESQVSWKPATLKSTLHDHLAGDEVLIVSNRQPYIHNRKGEA
                     IEVQVPASGVVTALEPIMRACSGVWIAHGNGSADRDVVDARAHVRVPPEAPSFDIRRV
                     WITPEEEAGYYYGFANEGIWPLCHLAHVRPVFRSSDWQQYQAINERFALAVVEEAKTD
                     NPVVLIQDFHLALVPKLVRDHLPHATIITFWHIPWPNPERYAICPWYVEILEGLLGSS
                     ILGFHTRFHCSNFINTVDRSLETRIDWDSSTISYGGKLTAVNPYPISIEWPGRMLASQ
                     PSVFECRKRIREIYALPLDHVVGLGIERLDYTKGILERFLAVERLLELEPQWIGRFSF
                     VQIAAPSRSKIEEYAQFRQQVENLAERINARFGREGYQPIQLLIRHHDVRDVMMHYRG
                     ADLCVVSSLHDGMNLVAKEFVAARDDEQGVLILSQFTGAATELPDALVVNPYNIDQCA
                     AAMHLALTMPPTEQRARMRSMRGIVQEF"
BASE COUNT         2017 a         2684 c         2910 g         1916 t
ORIGIN      
        1 cggcaccgtc acacgtccga ggtaggtagg atcagtcgcc ttataggcct cactgaggta
       61 acggaatcca ttgatgggat cctgggaatg acccggtcca ttgcgaaagg cccagccacg
      121 ttcgtctcga atcggatcga caacggtcat tccaacgacg tgttccagct gtttcaactt
      181 gcgtacgatg atcgtgcggt gtgcccaggg gcatgcccac gaaacgtaga gatgataccg
      241 gcctgaggcc gcggggtagc cggaggctcc atcagccgac acccatcctc gaaacctatc
      301 ctcctgcctt gtgaattcac ccttcgctga ttgttcgttt gggaattgcg gtcgaccagt
      361 catgagtggc tctgcaggcg agtatggcac agagcatggt acagacaaca gactaccggc
      421 ctctttccgc aaaagaaatg ccgggttgcg acaactgtgc tagggactca ccgcgttcaa
      481 gaacggtccg taatttgcaa aagtggcatg ttacaggact cgtccattcc aaagcctcga
      541 attgggttgt aaataagtag aagcaagcaa aagggacggc tttccgtgac ctcatgccac
      601 cagtcgtgcg cgtgaggcga agcgtgtcaa tgttgcgcac aacatggcag aattcaatcc
      661 cgcgaatagc aaacggttga gggaacaagc ggatcaattg agccgcacaa cttcacatca
      721 gctgcctaaa ctcaccccgc acagcgttga ggcgcttgtc catgagctcc gcgtacacca
      781 ggttgaattg gagctgcaat gccaggagct caaagatgcg caagctgaat tggaggaaag
      841 tagggaccgg tatcgtgagt tatatgaatc catccccatc gggtatgcga cgatcggcgg
      901 ctccggccgg atccacgact tgaatcccgc cggaatgtcc ttgctggggt tgccgtccac
      961 tctcagcaag ctccccaact tcatgatgtt tttcgtgtcg gagggcgatt cagacgtcgt
     1021 ggcgcggctg tgtcgcagcg cgctgaggga gcaacagcct ggcagtgctg aattacggat
     1081 gcgaaaatct gacgggagtc tcttcacggc ggctttgcac gccgagcccg tgctgacagg
     1141 agcgggaaag ggcgagcgcg tccgcatcac tttcaaagac gtgaccagga cccgggaggt
     1201 cgaggagtcc ttgcgacagc agcaactaga actggaagcg aaccgggtgg agctccagga
     1261 tctcatggcg aaactcttta ccgctcagga agaggaacgc aggcggattg cctgcgatct
     1321 gcatgacgat cactgccaac gcatcacggc catgattctg gaggccagtt caatcgaaaa
     1381 acttgtgaga gtggccatgc cgtcgcttct gcccaggatg ggtgcgctca aggaaaaact
     1441 caacggcatc ctggaggatt tccgccattt gacccatgag ctgcatccac gacacctcga
     1501 tactgtgtcc ctggcgtgct caatgcgcat gcacatcaag gaattcatcg actacacgga
     1561 tctgcgagtt gaattcaagg agcacgatgt tccggcgcac ctgccacttc ccatcacgat
     1621 ttgtctctat cgcctgctgc aagaaagtct tggcaacact cgcaaacacg cgaatgccaa
     1681 ggagatcgtc gtagagctga ccggcagaaa ggacgaagtc gagctgtccg tgtccgacga
     1741 cggagccgga tttaattttg cggattataa gaaaggtctg gggctcagaa gcatgcagga
     1801 gcgggtacgg ccgctccacg gccgcgtccg catcgacagc caacaaggcc gagggacgac
     1861 ggtcctgatc aatatcccgc tgccttccgg ggcctaacct gagagagaaa cggcggcagg
     1921 tcttctggcc ggtgtaacct cgttcacgac tacaacagcg tctttctgta aacccatgat
     1981 cgtcatcgac gaaaggagcg atcggactga cctctgcgtc cgtaagcgac cgcttctcat
     2041 cccacaatac agagacgtgc tcattgaatg gtcaccgacc gtcttgtgac aagatccgga
     2101 tatacgcaag cacgtcctgc atctccccgt cggtgagccg gccacgccag gcgtgcatgg
     2161 ggctgtagac gccgccgtgc tctatcatgc gcaagagcga ttcgtccgat ttcaagaacg
     2221 atccgggtcg atggaagttg gccggcggga tggtgagcaa gacggcattc ggtccgtcgc
     2281 catgcccatc tacgccatgg caactgaggc agtgtcgttc atagatggct ttgccacgcg
     2341 acagatcagg cggatactcc tgcgccatga gagggaccgg cagcgccaga ctaatcaaaa
     2401 ggaagaaacc agagcgtgaa atcttgccgg tcatacatca acggatcggc tgccgtttcg
     2461 ggtgttcagg gaattgtgag caaatgtcga acgcttttca agcgtctctg cgccctgatg
     2521 caggtgcccc ttgccgcaag gcgtgaatcc tgataattct caccgcagcc gcgatgccac
     2581 gcggcatacc cataccgctt gaagacacat ggagttctaa gaccatgagt gcctcacgtc
     2641 ccatcgtcgg catgctggta ggaggaggtc ccgcgccagg tatcaacagt gtgatcggcg
     2701 ccgtgaccat tcgcagccta ctcgggggct cggaggtact gggctttgtc gacgggttca
     2761 aatggctgat ggaaggaagc acagggcatg tgcatccgct ctctattgaa gaggtcagcc
     2821 gcattcattt tcgcggcggc tcacatttag gcacctcgcg agcgaacccg acgaaaaaac
     2881 cggagcacct ggatcgggtg ctctccacgc tcacgagttt gggcattacc cggctggtga
     2941 cgatcggtgg tgacgatacg gctttttccg cactcaagtt ggaggcgcgc tcagccgggc
     3001 gcatgcaggt cgtgcacgtg ccaaaaacca tcgacaatga tctggacctg ccctacggca
     3061 ttccgacctt cggctttcag acagcccgcc attatggagt cgagatattg aagaatctga
     3121 tggtggacgc ccgtacgacc tcgcgctggt acctcacggt caccatgggg cgaaaggccg
     3181 ggcatttagc gctcgggatc ggtaaagctg ctggcgccac gctgaccatc attcctgagg
     3241 aattcaggga gcgtccggtc aaattgcagc ggctcgtgga tgtggtcatc ggtaccatgg
     3301 tcaagcgcat tgccagcggg cggactgatg gcgtgatcgt cctggccgag ggattgatcg
     3361 aaatcatcga ccagaacgac ctgggcggtc tggaccacgt ggaacgcgac gagcacggcc
     3421 acttgcgtct gtcggaagtg gacatcggcg acctgctcag gcgcgaagtg acgaaaggaa
     3481 tgaaggcctt gggactgtcc gtcacggtcg tgtcaaagaa tgtcggctac gagcttcgat
     3541 gcgcggaccc gattccctat gacatggagt acacccgcga tctcggctat tgtgccgcgc
     3601 aatatctgct ggatggcggg acggcggcga tggtcacgat ccaggatggc cgctttgcgc
     3661 caatgccttt caaggagatg ctggaccccg tcaccggccg ggcaagggtc aggatggtgg
     3721 ataccgagtc gcaatcgtac cagatcgccc gtcagtacat gatccggctc aacgacgagg
     3781 atttcacgga tcaggaacgg ttgagccgcc acgcggctgt ggttggcttg tcgcccgatg
     3841 ccttcaagca gcggtttcag ttgaacagag gcaagcgcga ataaagagag ggtcagactc
     3901 catgcaggtg ttagtcacga ccgacggatc caaatacggc aagtgggcgc tcgaatgggt
     3961 gggacagttg ccgctcgccg aggagcccac cgtcaaggtg ctgcacgtcg tggatatcgg
     4021 gagacttcgc gcgcccttta tgttgcagcc ggcggtgatc ggtacggagc ggtacctgaa
     4081 agccgagatc acgaagctgc agacgcaggc cgagcgcaca aaacacgagg ccgctcgctg
     4141 gttgaagtct gcgggcttga aggggcgcgt ggtgatcgaa cgagggcccg tggcggccac
     4201 catcgtcaaa gcggccaagc gcggcgtcac tctcatggcc gtcggctctc gcggcttgga
     4261 tgcccttgat cgattcatgc tcgggagcgt gtccacgcac gtcatccacc atgtgtcctg
     4321 ctctgtatta gtcgtgaggg aagccccgcg accgatccgg cgtatcgtcc ttgccgtcga
     4381 tggctcaacg gcctccaaac gggccttgca gtttttgctg cgggctatgc gaccctcgac
     4441 tggccggcct tcgcaaggac cgatcagagt caccgtcacg cacgccatgc cattcttgaa
     4501 gtacccggag gtgcagcagg caggcaaggc cgtcgtggaa gcgtgttcga ccaaattggt
     4561 gcgagccggc tatgtggtcg agcagatgcc gataatagga aatcccgccg acaaaatttt
     4621 gaaaacagcc gaaacgcacg aagccgacct cattgtgacc ggcgccaagg gcctcggcgc
     4681 ggtcgggcga ttcctgctcg gcagcgtgtc gactagagtg gtacaacacg cggcctgttc
     4741 agtgctcgtg gtacgatgag ccaatggaac ggaatcaggt gagttcaccg cacacaagtc
     4801 aggcgggtcg gctgggcctt ggattggttg tcgtcgtctg tctcagcatt gttctgcaga
     4861 tgctgggagt cccggccccc ctgctcaatg ccggcgaaag cctcgatgcc ggcgaagcct
     4921 cagtctcgga agggttctcg ctgctcacga tctcctcgga ctgcatactt tcctcaacac
     4981 gtgtgctcgt tacataccgt gattcgtcgt ttcacgtgcc gattctggcc gcctcccttt
     5041 ttcatcctcc tctgatctag tcgtcctttc caccgcagca gtctatccac tcacagcact
     5101 tctgcacgac ggtcaagatc gggcaggaga ggaaggaacg agaatgacac ggatagctct
     5161 aagcgtgtgt gcggtagccc tgtgtgcctt cgcggcgtgc agtcatggga cggcgacgag
     5221 cagcggccga accgttcgaa cggtcgagat ttccgatcac gtcaagcccg gggtgatgta
     5281 tgcctcgccg ggagacgaag tgcggtggat gaatgcccgg gagaatcccg ttcgcgtcgg
     5341 ttttttgaac cgaaatctgc tggaagatca tcagtgccag aacggcatcg tcaacctgct
     5401 cggcgaagtg aacgatctga ttaccattgc tccgggagag tcgatcagtc tgtgtcccgt
     5461 tcgtgcaggc gacctcagat acaacgtatg gttcgatgcc gacaatccac gcggctcgat
     5521 gagcccgacc atgacggtca gaatccgtgg gggatgacag ttagaggacg tcgctccatg
     5581 gagggctgac agcaaacgcc gcgttgatga gaccgacgtg ggaataggct tgaggaaaat
     5641 tgccgcattg atccttcgtg cgcggaatga agtgctccga taggaggccg acgtgattgc
     5701 ccgccgtcac cacccgatcg agaatggccc gcgcgtggtt gagattgccc aggcgtgcca
     5761 gcgcttgggc gatccacagc gagcatacga cgaacgcccc ttccggcttg ccaaagtcgt
     5821 cctctcggac ataccggtag aagaatcccg tctccgaggc atcgaaagtg agcgagagct
     5881 ctcgcacgat ctggccaagc gtggaatcac acagcgcccg gtcgggatat ccgaggatcg
     5941 ccagttgcga cagcgccgca tcgtgcgtcg catcgtcggg tccgttgcgg acggcgccgt
     6001 cccgtacggc gcggagcagg gctcgggagg cacgttcgcg ctcagcggtc aaatcgagcg
     6061 tgatcgattt gaggaacccc gcacgtttga tgcgttccaa ccgttccaat ccggcccagc
     6121 acaagagatt cgtgaaggaa tgctcttgcc atccgccccg gatctcccag agacccgcgt
     6181 cctgacgccc gatgctcctg gcacagagct gcgccagatg agtcagaagg ccgtcgagat
     6241 cccgtgtgcg gagatcgagg aaccgttcgt cgaagaaaat cggcgtgaac gtgaggatca
     6301 tttcgccgta ggcatcattt tggacttgat cggccgccag gttgtggctg cggaccggcg
     6361 cgctgccttg atagccgctc caattcgcat gctcgatttc cggaagcggg agcccttgac
     6421 tgagcgtgta gacgggccgg aggcggtcac gggactgctc atgggtgagg gcgatgttca
     6481 ggaggaagtt caggaacgat tccatttcct cgaaatggcc cagattgttg aaggccgtga
     6541 gcgtgaagta ggcgtcacgc agccagcagt agcgataatc ccaattccgc tgaccgccta
     6601 cttgttcggg caggctggtc gtcatagccg ccaaaatcgc cccggtatct tcgtagcagt
     6661 ggagcttcag tgccagggcg gagcggatca cctcctgctg gtggagtaag gggacggaac
     6721 aatgcttgac ccagagccgc cagtagcggg tcgtttgttc gagaaagtcg tgtgtcactt
     6781 tgatgaggtc gtcctcgata cccagtcccc aggtcaggcc aaaataaaat tttcgcgtca
     6841 aggcgacggg cgtttcttcg cagagatagg tcaagggcat attcgtcagg aggcgcagcg
     6901 actcaccgcg gatgtcgtac cgtagatggt tgctgccacg gacgggcagc gcggcggttt
     6961 tttcccatcc gcacacggga cggcagctga cctgaatggc cggggtcccc ttcaggggct
     7021 cgacaagccg aaacagcgca gcagggcggt acatgcggcc gtactgctcg aatcgaggac
     7081 agaagtccgt tatccggaac gaatcgccgt tcgaaagcgt gacagtcgtc gtcaggatgt
     7141 ttgtattgga gagataggat tggctggtgg tcggctccgg ggaggatgaa agcgagctcg
     7201 taatggagaa atggccgcca tccgggtcca aaagcctccc gaagacggga ggactgtccg
     7261 gtcgggggag acacatccat tcgatagttc cggttgcatt gatgagggcc gaggtatgac
     7321 aattaccgat gagtgcgtag ggatacatgc tcccacaata ggggatttcg ctgctgaagt
     7381 aaacgagagg tgatgtatgc gtttgtccct gcgattcttg ctgcctctgg ccgtggtgct
     7441 ggccggcctc gcctatgccg tcattccctt ggttgacagc cttacgctga aatggttcgt
     7501 gagggatgtc gaaatccgcg ccgagctgat cggccgcatg gtcgaagggc cgctcggcga
     7561 cttgctcgca tcagaatcca ggacgaagct gcccgcctat ttcaatcgga tcattcagga
     7621 cgaacgacta tttgccatcg gattctgcga cctccgcaac cgcctgctct acaagacgca
     7681 ttcgtacccg gacgcgatcg cctgcgaagg taatgagacg atccgcgctg gcgaaacggc
     7741 cctcttgcag ttaccgcggg gagccgtgca tgtgtcggca gtcggcatcg aaggcaacgg
     7801 cggtcccgtc ggccgtttga tgctggttca tgacatgagc tgggtgcagc gtcgaagcac
     7861 ggatacgaag tggtatctct tctacttgtt tgccatcatc ggcgcggtga tctcatcggt
     7921 caccgtgctc gtggcacatc tgagttggcg tggctgggtc accggcgtcc gttcgatgct
     7981 gcgtggcgaa gggctgatcg cactgatcaa agacaagcac catggtcccg agttgcaacc
     8041 ggtcgccaaa gacctccgtg cgctcatcca ccatcttgag gcagacaagc ggatgaaaga
     8101 cgaaagccag gtgagctgga agcccgccac gctgaagtcc acgcttcacg accacttggc
     8161 cggcgatgaa gtgctcattg tctcgaaccg ccagccatac attcacaatc ggaagggcga
     8221 ggcgatcgaa gtccaggttc cggccagtgg agtcgtcacg gccctggagc cgatcatgcg
     8281 ggcctgttca ggcgtgtgga tcgcccatgg aaacggcagc gccgatcgtg acgtcgtcga
     8341 tgcacgcgct catgtccgcg tgccgcccga ggcgccctcg ttcgacatcc ggagggtctg
     8401 gattacgccg gaggaagaag cggggtatta ctatggattc gcgaacgaag gcatctggcc
     8461 tctttgtcat ctcgcccacg tccgtcccgt gtttcgatcg tctgattggc agcagtatca
     8521 agcgatcaat gaacgattcg ccttggcagt cgtggaggag gccaagacgg ataatccggt
     8581 cgtactgatc caggattttc acctcgctct ggtgccgaaa ctcgtccgcg accacttgcc
     8641 gcatgcgacg attatcacgt tttggcacat tccctggccg aaccccgaac ggtatgcgat
     8701 ctgcccctgg tatgtcgaaa tcctcgaagg tctgctgggc agcagcatct tgggattcca
     8761 cacccgcttc cattgcagta acttcatcaa cacggtggat cggtctctgg aaacgaggat
     8821 cgactgggac agctcgacga tttcctatgg gggtaaactc acggccgtca atccgtatcc
     8881 gatctcgatc gaatggcctg gccgcatgct cgcttcccaa ccgtcggtct tcgagtgccg
     8941 caagcggatc agggagatct atgcactgcc cctcgatcat gtcgtgggat tgggcatcga
     9001 acgtctcgat tatacgaagg gcattctgga acgctttttg gcggtcgaac ggctgctgga
     9061 actggagccc cagtggatcg ggcggttctc cttcgttcag atcgccgccc ccagccgttc
     9121 gaagatcgag gaatatgcgc agttccggca gcaggtcgag aatctcgccg agcgtatcaa
     9181 cgcgcgattc ggccgggagg ggtaccaacc aatccagttg ctgattcggc atcatgacgt
     9241 ccgagatgtc atgatgcatt atcgtggggc cgacttgtgc gtcgtcagca gccttcatga
     9301 cgggatgaat ctggtggcga aagaattcgt ggccgctcgc gacgatgagc agggcgtcct
     9361 cattctcagc caattcaccg gtgcggcgac agaattgccg gacgcactcg tggtcaatcc
     9421 gtacaacatc gaccagtgcg cggcggccat gcatcttgca ctgacgatgc ctccgacgga
     9481 acaacgggcg cggatgcgaa gcatgagggg catcgtgcaa gagttca
//
LOCUS       DASSCQ010000048         5928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1072181, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000048 DASSCQ010000000
VERSION     DASSCQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072181"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFH05_03580"
     CDS             complement(<1..680)
                     /locus_tag="VFH05_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677322.1"
                     /translation="MRIRKIRGRIVLAIVLVGCIPLVIGLGLAYVSGMQSLRDVIGGN
                     LQAVAVQAADRVTMLVQGEVRNARLLASAPLRVRLPVQVANSDYPPDQEGARLLIGHR
                     TLLWEQGGDHAASLLNGELSRFLLETKIRDGDKVIGLMILDRHGALVAASSEPDKYFF
                     GNESWWTLLGTGGDQLYLSGRIPAEEGAFRASDETLDIAVPILDDRQQQMIGAVIASY
                     RFDSLFAM"
     gene            complement(787..2022)
                     /gene="amrB"
                     /locus_tag="VFH05_03585"
     CDS             complement(787..2022)
                     /gene="amrB"
                     /locus_tag="VFH05_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HET6677323.1"
                     /translation="MTTAKDPQQYPVLRNLQFSPIKEGEEQYVVLWDPSGLSKEKLVL
                     PLNYFFIIQHFDGEHSLQEIGALYLKRFGEFLMPNKVEQLIGDLDAKLFLEGDRTEGA
                     KEQARLAYRHAPVRPAAFAGKVYEADGAKLRKQIDGFFASKEGPDFKPSDNKGKLIKG
                     LLAPTYDLKQAGPIYAWAYKEFQQAQQPDVVVIIGTAHAGLEHVFAVTDKDFETPLGL
                     MTVDRAVTDRLRAAVPDYFSEEVAHLSEHAIEFQLPFLQTNAGSATACRIVPILSSFS
                     AMSLTDPSVRAAVDRFLAALKEATADSGRTVCVIAAGDLAHLGMRYGDSAPPTDFSFH
                     RSMQYDLEMLKFVEELKAEDFAGYIQKEKDQRRISGFSPLYSLLRLIQAEQGQVLRYD
                     RGITDQYNSTVTYASMSFF"
     gene            complement(2092..2838)
                     /locus_tag="VFH05_03590"
     CDS             complement(2092..2838)
                     /locus_tag="VFH05_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677324.1"
                     /translation="MDATILGAWMATGLTLFIFSFLYQDNPLFKLAEHLYVGVSVGYT
                     IVKAYDTVIVHLIVKPIVEEGQIALLIPVAIGLLMLTRYVPRAAWMSRYAFAFIVGLG
                     SGLAIPRTISSFVLKQVEDTIRPLLFQAGDGLTFSFNLLNPASSLNAVIILIGVSTVL
                     FYFFFSIEHSGPGKLVARTGIVFLMIAFGAAFGYTVMARMSLLIGRLTDLIEFSDVSY
                     GRPTWWLLIVTIAVLVLLARRVPNRPPDLH"
     gene            complement(2870..3700)
                     /locus_tag="VFH05_03595"
     CDS             complement(2870..3700)
                     /locus_tag="VFH05_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677325.1"
                     /translation="MTFSERMLKIDRRIIFMVIGLCTLLPLLYPVGLPIKISPEVRSV
                     YEYIESLPERSVFLLSIDFDPASKPELHPQAIALLRHAFRKNLRVVAMTLWVSGTGMA
                     DQLLKQVSSEMGREPGRDYVFLGWSPGGSAVIINMGQDLYTAFPSDYEGKPTRGIPVL
                     EGVHSLKDVTYAISLGAGNPGVEAWYVYGKDKYKFELGGGCTGVIAPGLYPLLRSGQI
                     NGLIGGLRGAAEYESLIEEKGRAVAGMDAQSATHLAIIVLVAMCNLFYFSLRQQKGRG
                     "
     gene            complement(3735..4385)
                     /locus_tag="VFH05_03600"
     CDS             complement(3735..4385)
                     /locus_tag="VFH05_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677326.1"
                     /translation="MIFLRRRLPLLITMITGLLFAGQYYVPHPASEWLLTSATKWLQI
                     IGGFALVLGVTSLFHQHAVKIRRQEAGWGYSLVLYAGMLVTIAVGLWAGGKESLDGSM
                     TAFGWIYSYMMVPLQGTMFAILAFFIASAAYRSFRARSREAAVLLIAAVIVMMGRVPL
                     GEYLIPLSGDVSQWILNVLNASVRRAILIGVSLGAIALSLKIIFGVERSYLGGGKE"
     gene            complement(4382..5641)
                     /locus_tag="VFH05_03605"
     CDS             complement(4382..5641)
                     /locus_tag="VFH05_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HET6677327.1"
                     /translation="MRALLNFLTVIGFHAITAIAGALLSCSSAQAAPSAPASLHVIDT
                     PNDGGESVTVVWAPAPSDGPNAQYQVLIGEGGATDPAALKVVAEFPANTRYVKEIKAA
                     WWTRPAERAWHHYVVRSGKGVELKDGTAYTVTVAMREGDERALGPLLQATPEANWFNW
                     NQLNNLMLALGFGGLVFYSIRHAKRHDIFLRRIPGLDAVDEAIGRSTELGKPILYLTG
                     AHDMSDPSTIAAAVILGRVAKRTAAYETELLVPHRDPITMAVCQEITKQAYLEAGKPD
                     LFKEDANFFITADQFSYTAAVDGIMLRKKPAANFFMGSYFAESLLLTETGASTGAIQI
                     AGTDSDHQLPFFVTTCDYTLIGEELYAASAYLSREPIQIGTLRGQDIGKAIVLTVIMI
                     GTLLATIGAVTGSSWPQLFLDLFKDLK"
     gene            complement(5668..>5928)
                     /locus_tag="VFH05_03610"
     CDS             complement(5668..>5928)
                     /locus_tag="VFH05_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677328.1"
                     /translation="RPEIIIPRSSSVGQAPSEMQRAGIRLGDTVRIIRDPLFWKIGEV
                     SGLPSDLTRIPTESEVRVLEVTFAEGTRAVIPRTNIEVIEGA"
BASE COUNT         1325 a         1757 c         1671 g         1175 t
ORIGIN      
        1 atcatggcaa atagactgtc aaaacggtac gacgcgatga ctgccccgat catctgctgc
       61 tgccgatcgt caagaatggg aaccgcaata tcgagcgttt catcggaggc tctgaatgcc
      121 ccctcttccg cgggaattcg accgctgaga tacagctggt ccccccctgt gcccaaaagg
      181 gtccaccagg actcgttccc gaaaaaatat ttgtccggct cggaactggc cgccaccagc
      241 gctccatgtc ggtccagaat catgagtccg atcactttgt cgccgtcgcg gatcttggtt
      301 tcgaggagaa accgggagag ttcgccattc aacagggagg cggcatggtc cccgccttgt
      361 tcccagagca gggtccggtg gccgatcaag agcctcgcgc cttcctgatc gggaggatag
      421 tccgagttgg ccacttgaac aggcaagcgc acacgtaatg gcgcagaggc caacagcctg
      481 gcattacgta cctccccttg caccaacatc gtcacacgat cggccgcttg aacggccacg
      541 gcctgcaggt tgccgccgat cacatcccgc agcgattgca tgccggagac ataggccagc
      601 cccaagccga tcaccagggg aatacatccc accaggacaa tggccaagac aatgcgtcca
      661 cgaatcttgc gtattctcac cggctctctc ccctcactct tgggtattgt acgaaacgct
      721 gacgtcgaaa ctccacccat cggcatacgg tggctcggct ggagggaggt caaaggagcg
      781 gatggattag aagaacgaca ttgacgcgta cgtcaccgtc gagttgtact ggtccgtaat
      841 gcctcggtcg tatcgaagca cctgcccttg ttcggcctga atcaggcgaa gcaggctata
      901 gaggggtgag aagccggaga tccggcgttg gtccttttct ttctgaatgt atccggcaaa
      961 atcctctgcc ttcagttcct caacgaactt cagcatttcg agatcatact gcatggacct
     1021 gtggaaggaa aaatcggtcg gcggggcact gtcgccgtaa cgcattccca gatgggcgag
     1081 gtctccggcg gcaatgacgc aaaccgtccg cccggagtcc gcagtcgctt ccttcaacgc
     1141 cgcaaggaat cggtcgaccg ctgcgcgcac cgagggatcg gtcaggctca tcgccgagaa
     1201 ggatgagaga atgggcacga tgcggcacgc tgtcgccgag ccggcgttcg tttgcagaaa
     1261 cggcaattga aactcgatgg catgttccga gagatgggcg acctcttcgg aaaaatagtc
     1321 cggcaccgca gccctgagcc ggtccgtgac cgcacggtcc acggtcataa gtccaagcgg
     1381 cgtttcgaag tctttgtcgg tcacggcaaa cacatgctcg aggccggcgt gggctgtccc
     1441 gatgatcacg acgacgtccg gttgttgagc ttgttgaaat tctttgtagg cccaggcata
     1501 gataggccct gcctgtttga gatcatacgt cggcgcaagc agacctttga tgagcttgcc
     1561 cttattgtcc gatggcttga aatccgggcc ttctttggat gcaaagaagc cgtcgatctg
     1621 cttcctcagt ttggccccgt cagcttcgta gactttcccg gcaaacgccg ccggacgaac
     1681 cggagcgtgg cgataggcca accgggcctg ctccttcgcg ccttcggtgc ggtcaccttc
     1741 gagaaacagt ttggcgtcga gatcgccgat caattgctcc accttgttgg gcatcagaaa
     1801 ctcaccgaag cgcttcaaat acaacgcccc gatctcctgc agggaatgct cgccgtcgaa
     1861 gtgttgaatg ataaaaaagt aattcagcgg caggacgagc ttttccttac tcaatccact
     1921 ggggtcccag agcacgacgt attgctcttc tccctctttg atcggggaga actggagatt
     1981 ccgcaagaca ggatattgct gagggtcttt ggcagtcgtc atatctcctc gcaaggcaag
     2041 tcggcattct aacgaaccgc tttccagacc tgcaactgct ggatcgatgg tctagtgaag
     2101 atcgggaggc cggttgggga cgcgtctcgc caacaggacc agcacggcga tggtcacaat
     2161 cagcagccac caggtgggtc gaccgtagga gacatcggag aattcgatga ggtcggtcag
     2221 tcggccgatc aagagcgaca tgcgtgccat caccgtgtac ccgaatgccg cgccgaacgc
     2281 gatcatcaga aagacaatcc cggtcctcgc cacaagcttg cccgggccgc tatgttcaat
     2341 ggaaaagaaa aaatagaaga ggaccgtgct gacgccgatc aaaatgatca ccgcattcag
     2401 gctgctggcc ggattgagga gattgaagga aaaggtcaga ccgtccccgg cctggaacaa
     2461 caatggccgt atcgtatcct ccacctgctt gaggacaaat gaagaaatgg tgcgggggat
     2521 cgcaagcccg ctgccgagtc ccacgataaa ggcgaatgca tagcgcgaca tccaggccgc
     2581 cctggggaca tagcgcgtca gcatgagcaa gccgatcgcc accggaatga ggagcgcgat
     2641 ctggccctcc tcgacaatcg gtttgacgat caaatgcacg atgacggtgt cataggcctt
     2701 gacgatcgtg tacccgaccg agacgccgac gtacagatgc tcggcgagct tgaagagcgg
     2761 attatcctga tacaaaaaag aaaagatgaa taacgtcagc cccgtggcca tccatgcacc
     2821 caaaatggtg gcatccatat cgatcaccga taccggttga ggcgacgcct catcctcggc
     2881 ctttctgctg ccggagtgag aaatagaaga gattgcacat tgcgaccagc acaatgatcg
     2941 ccaggtgggt cgccgactgc gcgtccattc cggcgaccgc tcgccctttt tcctcgatca
     3001 agctctcata ttcggccgcc ccgcgcagtc ccccgatcaa accgttgatc tgcccgctgc
     3061 ggagcaacgg atagaggccg ggggcaatga cgccggtgca tcctccgccc aactcgaact
     3121 tgtatttgtc ctttccgtac acataccagg cctcgacccc cggattgccg gcgccgagac
     3181 tgatggcata ggtgacgtct ttcaagctat gcaccccttc cagtaccgga atacctctgg
     3241 tgggcttccc ttcgtagtcg ctggggaacg ccgtatacag atcctggccc atgttgatga
     3301 tcacggcgga gcccccgggg ctccatccga ggaacacata atcccgccct ggttcccgcc
     3361 ccatttcgct tgaaacctgt ttcaacaact gatccgccat tcccgtcccc gagacccaaa
     3421 gcgtcatggc aacgacgcgt aagtttttgc gaaacgcatg acgcagcaaa gcaatggcct
     3481 gggggtgcag ctctggcttg gaggccggat caaaatcgat ggagagcagg aacacggatc
     3541 gttcgggtaa cgactcgatg tattcgtaga cgctgcgcac ttcgggagaa atcttgatcg
     3601 gcaaccctac cgggtagagc aacggcaaca gcgtacagag tccgatcacc ataaagatga
     3661 ttcgtcggtc gatttttagc attcgttcgg agaaggtcat gccatgcctc ctctcgctgc
     3721 ttggtgtcca tgcctcactc tttccctccc cccaagtatg aacgttcgac tccgaagatg
     3781 atcttcaagg agagggcgat ggcgcccagg ctgacgccga tcaaaatggc acggcggacg
     3841 gaggcattca gtacattcag gatccactgc gagacgtcgc cgctcaaggg gatcaaatac
     3901 tcgccgagag ggacccggcc catcatgacg atcaccgccg caatcaacag aacggccgcc
     3961 tcccggctcc tggccctgaa cgatcggtag gctgcagagg cgataaaaaa ggccaggatg
     4021 gcgaacatcg tgccctgcaa cggcaccatc atatagctat agatccagcc gaacgccgtc
     4081 atgctgccgt ccagactctc ttttccgccg gcccagagcc ccaccgcaat cgtgacgagc
     4141 atgccggcat agagcacgag gctgtacccc cagcccgcct cctgacgcct gatcttcacg
     4201 gcatgctgat gaaacaggct ggtcaccccc aagaccaatg cgaacccgcc gatgatctgc
     4261 aaccacttgg tggccgatgt cagaagccac tcggacgccg gatgcggcac atagtactgg
     4321 ccggcaaaga ggagaccggt gatcatcgtt atcagcaagg gcaggcggcg gcggagaaaa
     4381 atcatttgag atccttgaag agatcgagga acaactgggg ccatgacgag ccggtcaccg
     4441 ccccgatcgt ggccaggagg gtgccgatca tgatgacggt caagacgatc gcctttccga
     4501 tatcctgtcc gcgcagcgtg ccgatttgaa tcggctccct ggagaggtag gcgctggcgg
     4561 catacagctc ttccccgatg agcgtgtagt cgcaggtggt gacgaaaaac ggcagttggt
     4621 ggtccgagtc cgtccccgca atttgaatcg ctccggtact ggctccggtt tcagtcagga
     4681 gcagcgattc ggcgaaatat gaccccatga aaaaatttgc ggcgggtttt tttcgcagca
     4741 tgatgccgtc cacggcggcg gtatagctga actggtcggc cgtgatgaaa aagttggcgt
     4801 cctccttgaa gagatcgggc ttcccggctt cgagataggc ttgtttggtg atttcctgac
     4861 agacggccat cgtgatcgga tcgcgatgcg gcaccagtaa ctccgtttca taggcggcgg
     4921 tgcgcttggc cacacgtccg aggatcaccg ccgccgcgat agtcgagggg tcgctcatgt
     4981 catgggcacc cgtcagatac agaatcggct tgcccaattc ggtggaccgg ccgatcgcct
     5041 catcaacggc atccagaccc ggaatgcgac gcaaaaaaat atcatgtcgc ttcgcatgcc
     5101 tgatgctata gaacaccagg ccgccgaagc ccaaggccaa catcaagttg ttgagttggt
     5161 tccaattgaa ccaattcgcc tccggcgttg cctgtaacag aggaccaagc gctcgctcgt
     5221 cgccttcgcg catcgcgacc gtcaccgtat acgctgttcc atccttcagt tcgacaccct
     5281 ttccgctcct caccacatag tggtgccagg cgcgctcggc gggtctggtc caccaagccg
     5341 ctttgatctc tttcacgtat cgggtattcg cgggaaattc tgccacgact ttgagagcgg
     5401 ccggatcggt ggcgccgcct tcgccgatca agacctgata ctgtgcattc gggccgtccg
     5461 acggagcggg agcccacacg acggtcacgc tttcaccacc gtcattcggg gtatcgatca
     5521 cgtgaagact ggcgggagcc gacggagcag cctgagccga tgagcaagaa agaagtgctc
     5581 cggcaatggc agtgatcgcg tggaacccaa tcaccgtgag gaagtttagc aaagccctca
     5641 ttccagctac accaactctg gcgactctta ggcgccttcg attacctcaa tattggtcct
     5701 gggaatgacc gctcgtgtcc cctcagcaaa ggtcacttcg agtacgcgaa cctcgctctc
     5761 ggtgggaatc ctggtgagat cggacggcag ccctgatact tcgccgattt tccagaacag
     5821 agggtcgcga atgatccgaa cagtatctcc tagccgaatg ccggctcgtt gcatctcaga
     5881 cggtgcctgt ccaactgatg acgagcgggg aataatgatt tcggggcg
//
LOCUS       DASSCQ010000049        15238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1836229, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000049 DASSCQ010000000
VERSION     DASSCQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836229"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFH05_03615"
     CDS             complement(<1..140)
                     /locus_tag="VFH05_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET6677329.1"
                     /translation="MAKAKYERKKPHVNIGTIGHVDHGKTTLTAALTKISADKGMAKF
                     IS"
     gene            complement(184..2265)
                     /gene="fusA"
                     /locus_tag="VFH05_03620"
     CDS             complement(184..2265)
                     /gene="fusA"
                     /locus_tag="VFH05_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6677330.1"
                     /translation="MARQAPLERTRNIGIMAHIDAGKTTTTERILYYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWKDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFDSVQGVEPQSETVWRQADKYQVPRIAFMNKMDRVGADFYFSVQTIIDRLGANPV
                     PIQIPIGRESEYRGSIDLITMKAYVYDDETLGAKYKIEDIPADLIDKAKEYREKMLEA
                     VAEFDDHVMEKYLNGQSLTEDEVRRVVRAGAIAMKVTPVLCGSAFKNKGVQQLLDGVV
                     DFLPSPVDVPPVKGIDPNTDKEVERKPSDSEPFSALAFKIMSDPFAGQLTYFRVYSGM
                     LKTGTPVLNVTKGTKDRIGRLLKMHANKREEIDAAHAGDIVAAVGLKGATTGDTLADE
                     KQPVLLEVMKFPEPVIAMAIEPKTKQDQEKMGFALQKLAQEDPSFRVRTDEETAQTII
                     AGMGELHLEIIVDRLLREFKVEANVGKPEVAFRETIRRKAEAESKYIKQTGGRGQYGH
                     VVMTVEPSESGKGLEFVNKVVGGAIPREYIPAIEKGVKERMENGVIAGYPLRDIKVTV
                     IDGSYHEVDSNEMAFKIAASMGFSDACKKADAVLLEPIMKVEVLVPQEFMGDVIGNLN
                     GRRGKVQGMKVRAGAQAIEASVPLMEMFGYATDLRSRTQGRATYSMEFDRYEQVPKQI
                     SEAIIAKYRGE"
     gene            complement(2376..2846)
                     /gene="rpsG"
                     /locus_tag="VFH05_03625"
     CDS             complement(2376..2846)
                     /gene="rpsG"
                     /locus_tag="VFH05_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET6677331.1"
                     /translation="MPRSRFLGQREPLPDVRYRDKIVGKFLNILMEGGKKSTAERICY
                     GAFDVIQEKTGGDPLKVFRSAIDNVKPIVEVKSRRVGGASYQVPVEIRPARRMSLALR
                     WLSEYARTRGGKSMREKLAAELLDASNNTGAAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2884..3255)
                     /gene="rpsL"
                     /locus_tag="VFH05_03630"
     CDS             complement(2884..3255)
                     /gene="rpsL"
                     /locus_tag="VFH05_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET6677332.1"
                     /translation="MPTINQLVRKGRQLAHAKTKSPALKSCPQKRGVCLRVYTSTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DAVGVADRKQGRSKYGAKRPK"
     gene            complement(3369..7493)
                     /gene="rpoC"
                     /locus_tag="VFH05_03635"
     CDS             complement(3369..7493)
                     /gene="rpoC"
                     /locus_tag="VFH05_03635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET6677333.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVCVDAGQTDLTEKDLVTEEKLRQLQSEYAANAYKV
                     GIGADAIRELLRKIDINAKWDEIKAKAKVSTSAAMKKKFAKQLKVIEAFRKSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERVGAKGEGKV
                     FGSTEEVRIAFDAREMEEHARIKVRIDGTLVQTTVGRVILSEVLPAGMPFAYANKLMT
                     KKEVTKLIDSVYRQSGHRETVMFLDKIKDAGFTYATRAGVSICIDNMHIPSKKEELIG
                     KAQREVNEIERQYAEGLITNGERYNKVIDIWAHVTEQVAGEMMKELGAGGDPNKDEAF
                     NPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTH
                     GARKGLADTALKTANSGYLTRRLVDIAQDVIISEIDCGTTDGIFVSALVEGGEIIQPI
                     EERILGRLAAEDIRDPVTGEVIVSFNEEITEERTKDVVEAAVDRVKIRSVLTCQSRRG
                     VCRACYGRDLARGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLE
                     AKHAGRMKFMSFDAKKNADVHNGGIAVRNKENEWVVMNRNAKIAIVDENGREREKYPV
                     VYGAKIKIKDGDRVELAQKLVEWDPYSLTILTEVGGKVAYGDIVEGVTMKEEFDEVTG
                     LSRKVIIEHSGATLRPRVSIKDEGGKTAKVSGGSNTVARYLLPVGAHIFVEKGAAVHP
                     GDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEQAVISEIDGEVSYGGFVKGQRKV
                     LVDNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLMDGSANPHDILDVLGPNELQKYLV
                     DEVQDVYRLQGVSINDKHIEIIVRQMLRKVRIEDPGDTEFLPGSQVSKMLFEEENERV
                     LKKDGKPALGKPVLLGITKAALTTDSFISAASFQETTRVLTEAAINGREDNLLGLKEN
                     VIVGRLIPAGSGFEEYRDTFVISPKPEPVVVGSTEPAALPREGAAAVTGEGAGG"
     gene            complement(7591..11547)
                     /gene="rpoB"
                     /locus_tag="VFH05_03640"
     CDS             complement(7591..11547)
                     /gene="rpoB"
                     /locus_tag="VFH05_03640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET6677334.1"
                     /translation="MPETTLQEFVERKDFSRIRTSIDIPDLIEIQKRSYEEFLQFEVE
                     PERRKDHGLQAALASVFPIPDYNNTAILEFTSYTLGTPKYDERECLEQGMTFAVPLKL
                     RVRLVVFDKEDKGPKKKVLDVREQEVYVGELPLMTERGTFIVNGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGFSSDDLLKMYYPVEEIRVNKGKLMRKLDPEIHQGMRCSTEVVDKGGKEPLVREGGK
                     LTKGIIAKLKAAGVKEIPVAPSELVGRAVLSEVVDTKKNKLVEKNQRLTAEIIEKILE
                     SEIEEFKVIFFDAATSTPVILDTLEMERTVSKEEAMVEIYRRLRPGETPSVETARALF
                     DNLFLNSKRYDLSPVGRLKLNKKLLLDLPLEQRTLTSQDIVEVIRYLVNLKMGKGEID
                     DIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVAA
                     VKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRIC
                     PIETPEGPNIGLITSLATYARINEFGFIEAPYRRVEKGRVTDQIEFLSAIEGDKFLIA
                     QANSKVDGSGRLVSETVSARSGGDFVTITPDRVEYMDVSPKQVVSVATALVPFLEHDD
                     ANRALMGSNMQRQAVPLLKSEAPLVGTGMEAVVARDSGYVVQARRSGVVESVDATRIV
                     VRSDSKEGRKRNDTGLDVYDLIKFQRSNQNTCITQTPVVRIGQPVKKGQVLADGPAID
                     QGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGKE
                     DITRDIPNVGEEALRNLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAIF
                     GEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIESEDTMRLQRDHHEEL
                     RIIDEEKTKKVRKLLLGKVVGRDLMDPESGDVILKKKGKLTAEILKRLSDESVRYIIL
                     SDPDEQKELEDVERRAKEQVEILQTLYDEKVGRLKRGDELPPGVIKLVKVYISMKRKI
                     QVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHLG
                     WAAKALGIKVASPVFDGASEKEIKDLLKKGKLPASGQTLLMDGRTGEAFGSPVTVGYM
                     YVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASTLQ
                     EFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKTQD"
     gene            complement(11625..12026)
                     /gene="rplL"
                     /locus_tag="VFH05_03645"
     CDS             complement(11625..12026)
                     /gene="rplL"
                     /locus_tag="VFH05_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET6677335.1"
                     /translation="MSATATKVSQEELIKAIESMSVLDLAELVKGLESRFGVTAAAPV
                     AIAAAPAAGGAAAAPAEEKTAFDVILASAPADKKIQVIKVVRELTNLGLKEAKDLVEG
                     APKPVKTGATKEESDAMKKKLEESGAKVEIK"
     gene            complement(12054..12560)
                     /gene="rplJ"
                     /locus_tag="VFH05_03650"
                     /pseudo
     CDS             complement(12054..12560)
                     /gene="rplJ"
                     /locus_tag="VFH05_03650"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            complement(12615..13307)
                     /gene="rplA"
                     /locus_tag="VFH05_03655"
     CDS             complement(12615..13307)
                     /gene="rplA"
                     /locus_tag="VFH05_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET6677336.1"
                     /translation="MGKKMKAALAKMEARPYALRDAVALVKGTAYAKFDESVDLAIRL
                     GVDPKRSDQMVRGTTSLPHGTGKKVRVLVFAKGEKEQEARQAGADFVGSDDLMERIKG
                     GWMDFDYAIATPDLMASVGKLGKQLGPRGLMPNPKTGTVTFEVGKAVGEIRKGRVEYK
                     VEKAGIVQVPVGKVSFQPDQLFDNASAVLEAVVKAKPASCKGRYLKSVTISSTMGIGV
                     KLDAIALAKQWS"
     gene            complement(13331..13756)
                     /gene="rplK"
                     /locus_tag="VFH05_03660"
     CDS             complement(13331..13756)
                     /gene="rplK"
                     /locus_tag="VFH05_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET6677337.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKEGDSIIPVIITVYKDRSFTFIMKTPPASDLLKKAAGIIKGSGVPQKDKVGKITR
                     KQLTEIAQKKMADLNAADLEGAVKIIEGTARSMGVVVQG"
     gene            complement(13769..14344)
                     /gene="nusG"
                     /locus_tag="VFH05_03665"
     CDS             complement(13769..14344)
                     /gene="nusG"
                     /locus_tag="VFH05_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET6677338.1"
                     /translation="MSEAAPTNDPKVGMGKNWYVIHTYAGFEGRVKTSLLERANQMAL
                     TEKMGQVLVPTEDVIEIKDGKRRTSRRKFFPGYVLVELETPVTDETLQLIKETPKVTG
                     FVGGGAQPTPLTNEEVAELLKQVDAGSAGPREQVKFIKTDNVRIVDGPFLGFNGVVDE
                     VDQDHNRLKVMVSIFGRSTPVELGFLQVERI"
     gene            complement(14341..14535)
                     /gene="secE"
                     /locus_tag="VFH05_03670"
     CDS             complement(14341..14535)
                     /gene="secE"
                     /locus_tag="VFH05_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET6677339.1"
                     /translation="MFKRMIDNVKEFLGDVRTELKKVSFPTKAETIGSTTVVIVFCVL
                     MSIYLSVIDSFLSWLVGKII"
     gene            complement(14715..14789)
                     /locus_tag="VFH05_03675"
     tRNA            complement(14715..14789)
                     /locus_tag="VFH05_03675"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14754..14756),aa:Trp,seq:cca)
     gene            complement(14857..15006)
                     /gene="rpmG"
                     /locus_tag="VFH05_03680"
     CDS             complement(14857..15006)
                     /gene="rpmG"
                     /locus_tag="VFH05_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET6677340.1"
                     /translation="MREIIDLACTACKQRNYSTTKNKKNDPDRLERNKFCKFCRKHTP
                     HKEVK"
     gene            complement(15031..>15238)
                     /gene="tuf"
                     /locus_tag="VFH05_03685"
     CDS             complement(15031..>15238)
                     /gene="tuf"
                     /locus_tag="VFH05_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET6677341.1"
                     /translation="YRPQFYFRTTDVTGVVSLNPGVEMVMPGDNVSVTAELISPIAMD
                     QGLRFAVREGGKTVGSGVVTEILA"
BASE COUNT         2919 a         4745 c         3860 g         3714 t
ORIGIN      
        1 tagctgatga acttcgccat ccccttatcc gcgctgatct tcgtcaaggc cgccgtcaac
       61 gtggtcttcc catggtccac gtgcccgatc gtcccgatat tcacatgcgg cttcttgcgc
      121 tcatatttcg ccttcgccat acctcaactc cttctcagca agtgtcgtca gtcggatcag
      181 ccgttactcg ccacggtact tggcaataat ggcttctgaa atctgtttag gtacctgttc
      241 gtatcgatca aattccatac tgtatgtggc gcggccctgt gtgcgggagc gaaggtccgt
      301 cgcatagccg aacatttcca taagcggtac cgacgcttcg atcgcctgag caccggcacg
      361 caccttcatg ccttgaacct tgccgcgtct gccgttgagg ttaccgataa catcgcccat
      421 gaattcctga ggcaccagca cctcgacttt catgatgggc tcgagaagca ccgcatcagc
      481 cttcttgcag gcatcggaaa atcccatcga cgcggcaatc ttgaaagcca tttcgttcga
      541 atcgacctcg tggtacgacc catcgatgac ggtcaccttg atatcgcgca acggatagcc
      601 ggcaatcacg ccgttttcca tacgctcttt cacgcctttt tcgatcgccg ggatatattc
      661 cctggggatg gcccctccga cgaccttgtt gacaaattcc aaccctttac cggactccga
      721 tggctcgacc gtcatcacca cgtgaccata ctggccgcga ccgccggtct gcttgatgta
      781 cttcgattct gcttcggctt ttcgccggat ggtttcgcgg aaggcgactt cgggcttgcc
      841 gacgttcgct tcgactttga attctcgcag cagccggtcc acgatgattt ccaggtgcag
      901 ttcgcccatc cccgcaatga tagtttgcgc cgtttcttca tcagtgcgca cccggaagga
      961 cgggtcttcc tgtgccagct tttgcagggc aaagcccatc ttttcctgat cctgcttggt
     1021 cttcggttca atggccatgg caatgaccgg ctccggaaac ttcatcactt cgagcaggac
     1081 aggctgcttt tcatcggcca acgtgtcacc ggtggtggcg cccttcagcc caaccgcggc
     1141 cacaatgtca cccgcatggg ccgcatcgat ttcttcgcgc ttattcgcat gcattttcag
     1201 cagacgtcca atgcgatctt tcgtgccctt cgtgacgttc agcaccggcg ttccggtttt
     1261 cagcattccg gagtacaccc ggaaataggt gagctgcccg gcaaacggat cggacatgat
     1321 tttgaatgcc aaggcggaaa acggctcgct atcggaaggc ttccgttcca cttccttgtc
     1381 cgtattcgga tcgatgcctt tgacgggggg aacatctaca ggggaaggca ggaagtcgac
     1441 gacaccgtcc agcagctgct gaacgccctt gtttttgaag gccgagccgc acaacacggg
     1501 cgtcaccttc atcgcaatgg cgccggcgcg cacgactcgc cggacctcgt cttccgtcaa
     1561 ggactggccg ttaagatatt tttccatgac gtgatcgtca aactctgcaa ccgcttcaag
     1621 catcttttca cgatactctt tggccttgtc gatcagatcg gccgggatgt cttcaatctt
     1681 gtatttggcg cccagcgttt catcgtcgta gacgtaggct ttcatcgtga tcagatcaat
     1741 cgacccgcga tactcggatt cccgtccgat gggaatttgg atcggcaccg gattggcccc
     1801 caatcgatcg atgatggtct gcacactgaa gtaaaagtcg gcaccgaccc ggtccatctt
     1861 gttcatgaag gcgatgcggg gcacttgata cttatccgcc tgtcgccaca cggtttccga
     1921 ctgcggttcg acaccctgca cggaatcaaa tacggccacc gcgccgtcga ggacgcgcaa
     1981 cgaccgctcc acttcgatgg tgaaatccac gtgaccgggc gtatcaatga tattgatgcg
     2041 atggtccttc cagaaacagg tcgtcgccgc cgccgtgatg gtaatgccgc gttcgcgctc
     2101 ctgctccatc cagtccatcg tggccgcacc ctcatggacc tcgcccatct tgtgcgtcat
     2161 accggtgtaa tagaggatgc gctccgtcgt cgtcgttttg ccggcatcga tgtgcgccat
     2221 gatgccgatg tttctcgttc gttccagtgg tgcttgcctt gccatggtat ccctttaaaa
     2281 acacacgtgc tgcaggatga gccgaacacc gctgcgattc aggggcaccc gcgtctgcgg
     2341 atcgcagcac ggctcaactg cagcacgaaa cgacgttacc agcgatagtg cgcgaaggcc
     2401 ttgttcgcct ccgccatccg atgcacgtct tcccgtttct tgaccgcggc ccctgtatta
     2461 ttcgacgcgt ccaacagctc ggccgcgagc ttttcccgca tgcttttgcc gccccgcgtc
     2521 ctcgcatact ccgataacca ccgaagggcg agagacatcc ggcgcgccgg acgaatttcg
     2581 accggcactt ggtacgaagc cccgccgact cgtcgggatt tcacttcgac gatcggtttg
     2641 acattgtcga tcgccgatcg aaatactttt agtggatccc cacccgtctt ttcctgaatg
     2701 acgtcgaagg caccgtaaca aatgcgctca gccgtactct ttttgccgcc ttccatgagg
     2761 atgttgagaa atttccccac gatcttgtcg cggtaccgaa catctggcag cggctctctc
     2821 tgtcctaaaa atctgcttcg tggcatggtt gattactttc gtgagccctg atgaagtagg
     2881 tccctatttc gggcgcttgg cgccatattt ggagcggccc tgcttccgat cggcaacgcc
     2941 gacggcatcg agcgcgcctc gcacgatgtg atagcgcacg cccggcaagt ccttgacgcg
     3001 gccgccgcga accagcacga ttgaatgttc ttgtaagtta tgcccgacgc cgggaatgta
     3061 ggtcgtaacc tccattccgt tggtcaatcg cacgcgtgcc actttgcgaa gcgcggaatt
     3121 cggcttcttc ggtgttgatg tgtatacgcg caggcagacg cctcgttttt gcggacaaga
     3181 cttgagcgcc ggacttttcg tcttggcatg ggccagttga cggccctttc tcactaactg
     3241 attgatggtt ggcatcgttc ctgtctttcg gcttcaacgc aataggttcc taccggcccc
     3301 ttcaagggca aagcccgcgg attatagtga tgcccgcggg cgctgtcaag cgacgaaccc
     3361 cctgaccgtt acccgcctgc accttcgccg gttactgcag ccgccccttc acgcgggagc
     3421 gcagcaggct ctgtcgaacc gaccacaacg ggctccggtt tcgggctgat gacgaaggtg
     3481 tcgcgatatt cctcaaaccc gctgcccgca ggaatgaggc gaccgacgat cacattctcc
     3541 ttcaatccaa gaagattgtc ctctctgcca ttgatcgccg cttcggtcag cacacgagtg
     3601 gtctcctgga acgacgctgc agagatgaag ctgtcggtcg tcagtgccgc cttcgtgatg
     3661 ccaaggagaa cgggtttgcc gagcgccggc tttccgtcct tcttcaacac gcgttcattt
     3721 tcttcttcaa agagcatctt gctcacctga ctacctggca agaactccgt gtcgccggga
     3781 tcttcgatcc gcactttgcg caacatctgg cgcacaatga tctcaatgtg cttgtcgttg
     3841 attgacacac cctgcagacg gtaaacgtcc tgcacttcgt cgaccagata cttttgcagt
     3901 tcgttgggtc cgagcacgtc gagaatatcg tgtggattcg cggaaccgtc catcaacggc
     3961 tctccggccc tcacccaatc gccctcgtgg acgttgacgt gcttcccctt gggaatgaaa
     4021 tactctttga cgtcgcccat cttgttgtcg accaggacct tgcgttggcc tttgacgaac
     4081 ccaccgtacg acacttcgcc gtcgatttcg ctgatgacgg cctgttcctt cggctttctc
     4141 gcctcgaaca actcagccac acgcggcaaa cctccagtga tgtcttttgt cttggtcgtt
     4201 tcccgaggaa tcttcgccag cacatcaccc ggatggacag ccgcgccctt ctcgacaaag
     4261 atgtgtgcgc cgacgggcaa cagatagcgg gccacggtgt tgctgccgcc cgataccttc
     4321 gccgtttttc cgccttcgtc cttgatcgac acacgaggcc gcagcgtcgc tccggaatgc
     4381 tcgatgatca ccttgcgtga gagacccgtc acctcgtcga actcttcctt catggtgacg
     4441 ccttcgacga tgtcgccgta agccactttc ccgccgactt cggtcaaaat cgtcaacgag
     4501 tatgggtccc attcgacgag cttctgcgcc agttcaacgc gatctccatc cttgatcttg
     4561 atcttggcgc cgtagacgac cgggtacttc tcgcgctcgc ggccgttctc gtccacgatg
     4621 gcgattttcg cgttgcggtt catcacgacc cattcatttt ccttgttccg cacggcaatg
     4681 ccgccgttgt gcacgtccgc attcttcttc gcatcaaagc tcatgaactt catgcgaccg
     4741 gcatgcttgg cttccaggac ggtctgctcg acgaccttgc tcgccgtacc gccgatatgg
     4801 aatgttcgca tcgtcagctg ggtacccggc tcgccgatag attgcgcggc aatgacaccg
     4861 accggctcgc ccttctcgac gagacgcccg cgcgccagat cgcgcccata gcaagcccgg
     4921 cagacgccac gtcgggactg gcacgtcagc acggaccgga tcttgacccg atccaccgcg
     4981 gcttcgacca catccttcgt gcgctcctcg gtgatctctt cattgaagga gacaatcacc
     5041 tcacccgtga ccgggtcacg gatgtcctcg gcggccaacc gtccgaggat acgctcttcg
     5101 atgggctgga tgatttcccc accttcgacc aaggcgctga caaaaatccc gtccgtggtc
     5161 ccgcaatcga tttcactgat gatgacatcc tgcgcaatgt cgaccagtcg gcgagtgaga
     5221 tagcccgaat tcgccgtctt cagtgcggtg tcggccaacc ctttacgcgc gccgtgcgta
     5281 gagatgaagt attgcagcac ggtcaggcct tcacggaaat tcgccgtaat cggcgtctca
     5341 atgatttcgc ctgacggttt cgccatcagt ccgcgcatgc cacccagctg acgaatctgc
     5401 tgggaactgc ctcgggctcc tgagtcggcc atcatgaaga tggggttgaa ggcttcgtcc
     5461 ttgttcggat ctccgccagc gcccaactcc ttcatcatct cacccgccac ctgttcggtg
     5521 acatgggccc agatgtcgat gaccttgttg tagcgttctc cgttggtgat caatccttcg
     5581 gcatattgcc gttcaatctc gttgacttcc cgctgcgcct tgccgatcag ttcttccttc
     5641 ttgctgggaa tatgcatatt gtcgatgcag atcgacacgc cggcgcgagt ggcataggtg
     5701 aacccggcgt ccttaatttt gtccaagaac atgacggttt cccgatgccc tgactgacga
     5761 tagacggaat cgatgagctt ggtcacttcc ttcttcgtca tcaatttatt ggcatacgcg
     5821 aacggcatcc ccgcaggcaa cacctcggaa agtatgaccc ggccgaccgt tgtctggacc
     5881 aaggtcccat cgatgcgaac cttgatgcgg gcgtgctcct ccatctctct cgcgtcgaag
     5941 gcaatccgca cttcctcggt ggacccgaag accttgccct cgcccttggc cccgacccgc
     6001 tctttggtca accaatagca acccagcacc atatcctgcg acggtacggc gataggcttg
     6061 ccgttggccg gcgacagaat attgttgatc gacatcatca atacccgggc ttctacctgc
     6121 gcttccacgg acaacggcac gtgcacggcc atctgatcgc catcgaagtc ggcgttgaac
     6181 gccgcgcaga ccagggggtg caaccggata gccttgcctt ctaccaatac gggatcgaag
     6241 gcttggatgc ccaatcgatg gagcgtcggc gcacgattga gcagcacggg atgctcacga
     6301 atcacttcgt cgagcacatc ccagacttcc ggccgctctt tctcaaccaa gcgcttggcg
     6361 ctcttgatcg tcgtcgcggc gccgcgctct tccaatttgt ggaagatgaa cggcttgaac
     6421 agttccagcg ccatcttctt cgggagaccg cattgatgca gccgcagctc tggaccgacc
     6481 acgatgaccg tgcggccgga atagtccacg cgtttaccga gaagattttg acggaaccgc
     6541 ccttgcttcc ctttgagcat gtcgctcaag gacttgagcg gacgcttgtt cggtccacga
     6601 atcgcccgac cacgccgacc gttgtcgaac aacgcatcga ccgcttcctg taacatgcgc
     6661 atttcatttc ggatgatcac gcccggcgcc ttcaactcca tcagacgctt caaccggttg
     6721 ttccggttga tgacgcgccg atacagatcg ttcaagtccg aggtggcgaa gcgaccgccg
     6781 tccagcggca cgagcggacg taattccggc ggcagaaccg ggatcacatc catgatcatc
     6841 cactcgggct tgttgccgga cttgcggaag gcttcgatga ctttgagctg cttcgcaaat
     6901 tttttcttca tcgccgcgga ggtggagact ttggccttcg ccttgatctc atcccacttc
     6961 gcgttgatgt cgatctttcg cagcagctcc cggatggcat cagcgccgat gcccactttg
     7021 taggcgttgg cggcgtattc ggactggagc tggcgcagct tctcttcggt cacgaggtcc
     7081 ttctccgtca aatccgtctg cccggcatca acacacacgt agctttcgaa atacagtatc
     7141 ttttcgagct gcttcaggct catgtcgagt aacgttccga ttcggcttgg tacccctttc
     7201 aaaaaccaga tatgcgccac cggcgcagcc aattcgatat ggcccatccg ttcccggcgg
     7261 actttggact gaatcacttc aaccccgcac ttgtcgcaga cgatcccacg atgtttcatc
     7321 cgcttatact taccgcaatt gcattcccag tccttaatgg gaccgaagat ctttgcgcag
     7381 aacagaccgt ctttttccgg cttaaaggat cgataattga tcgtttccgg cttcttcact
     7441 tcgccgtagg accaggatcg gattttttcc ggcgacgcaa tgcggatgcg catcgaatcg
     7501 aaggagaccg aatcccgcgg cttttcgaat aatgtgtata cgccttccaa agtaatgacc
     7561 tcctctgacc ggcagtgccg gctcaagtgg ttagtcttgc gtcttgacca actcgacgtc
     7621 caatcccaaa ctctgcagct ccttgaccaa gacgttgaac gactcgggca atccgggctc
     7681 gaggaaaggt tcgcctttca cgatcgcttc atacattcgc gatcgcccgg gcacgtcgtc
     7741 ggatttcacc gtcaaaaact cctgcagggt cgaggcggct ccgtatgcct gcagcgccca
     7801 cacttccatt tcccccaacc gctggccgcc gaactgagcc ttgccgccta acggctgctg
     7861 cgtgacgagt gaatacggcc cgatcgaacg cgcgtgaatc ttgtcgtcga ccaggtggtg
     7921 gagcttcaac acatacatgt agcccaccgt caccgggctg ccgaaggctt cacccgtccg
     7981 cccatccatg agcagcgttt gtccgctggc tggtaatttg cccttcttta gcagatcctt
     8041 gatttccttt tcagaggcac cgtcgaagac gggactcgcc actttgatgc caagcgcttt
     8101 cgccgcccac cccaagtgcg tctccaagat ctgtccgacg ttcatacgtg acggaacgcc
     8161 cagcgggttc aagacgatct ccaccggcgt cccatcgggc agatatggca tgtcctcttc
     8221 gggcaacacg cgggacacga cgcccttgtt gccgtgacgg ccggccattt tgtcgccgac
     8281 ctggatcttc cgcttcatcg agatgtagac cttcacgagc ttgatgacgc ccggcggcaa
     8341 ttcgtcgccc cgttttaatc gtccgacttt ttcgtcgtag agcgtctgaa gaatttcaac
     8401 ctgttccttg gccctgcgct ccacatcctc cagttccttt tgttcgtccg gatcgctgag
     8461 aatgatatag cgcacggatt catcggagag gcgtttcaag atctcggccg tgagcttgcc
     8521 tttcttcttc agaatgacgt caccgctctc aggatccatc aaatcccgcc cgacgacctt
     8581 gcccagcaac agcttccgaa ccttcttggt tttttcttcg tcgatgatcc gcagctcctc
     8641 gtgatggtcg cgttgaagcc gcatggtatc ttcgctctcg atgctcttcg accgttcgtc
     8701 cttatcaagg cccttgcgcg aaaagatttt cacgtcaacg acaatacctt cgacccctgg
     8761 ggggaccgtc aaggatgtgt ccttgacgtc accggctttc tcaccgaata tggcgcgcaa
     8821 aagtttttct tccggcgtga gctgggtttc gcccttgggc gtgacctttc cgaccaggat
     8881 gtcgcctggc tttacctcgg caccgatgcg aatgatgccg ctttcgtcga gattccgcag
     8941 ggcctcttcc ccgacgttcg gaatatcacg cgtaatgtct tcctttccaa gcttcgtatc
     9001 gcgcgcttcg acttcgaact cttcaatatg gatggatgtg aacgcatcct cccgaacgag
     9061 cttctcactg aggagaatgg cgtcttcgaa gttgtagccg ccccacggca tgaacgcgac
     9121 aaggacgttt ttccccaagg ctagttcgcc ttgatcaatc gccggcccgt cggccaggac
     9181 ctgtcctttc ttgaccggtt gaccgatccg cacgaccgga gtttgcgtga tgcaggtatt
     9241 ctggttggat cgctgaaact tgatcagatc gtagacatcc aaacccgtat cgttccgttt
     9301 ccgcccctcc ttactatcgg agcgcacgac gatccgcgtc gcatccacac tttcgacgac
     9361 gcctgaccgc ctggcttgga cgacatagcc cgagtcgcgc gccacaaccg cttccatacc
     9421 ggtgccaact aacggcgctt ccgatttgag cagcggcacc gcttgccgtt gcatattgga
     9481 gcccatcaat gcgcggttgg cgtcgtcgtg ctccaggaac ggcacgagag ccgtcgctac
     9541 gctgaccacc tgcttcggcg acacgtccat gtattccaca cggtccgggg tgatcgtcac
     9601 aaaatcgccc ccggatctcg ccgaaaccgt ttctgatacc aaccggccgg agccgtccac
     9661 cttggagttg gcttgagcga tcaggaactt gtctccctcg attgcggaga gaaattcgat
     9721 ctgatcggtg acacggccct tctccacgcg ccggtaaggc gcttcgatga agccgaactc
     9781 gttgatcctc gcgtaggtcg ccagcgaggt gatcaggccg atattcggac cttccggcgt
     9841 ttcgatcggg caaatccggc tgtagtgaga ggggtgcacg tctcgcacct cgaatcccgc
     9901 gcgctccctg gtcaagccgc cggggcccag tgccgagagg cgtcgcttgt gcgtgatttc
     9961 agccaacgga ttcgtttggt ccatgaactg cgataactgg ctgctgctga aaaactcttt
    10021 aaccgcagcc accaccggct tggcattgat caagtcgtgt ggcaataccg tctccatgtc
    10081 gaggagattc atgcgctctt tgatgctgcg ctccatcctg accagaccga gacggaattg
    10141 attttccagc aattcgccga ccgaccggac ccgccgattt cccaagtggt cgatgtcgtc
    10201 gatttctcct ttccccattt tgaggttcac gaggtagcgg atgacttcga cgatatcctg
    10261 cgaagtgagc gtccgttgtt cgagcggcag gtcgagcaac aacttcttgt tgagtttcaa
    10321 tcgtccgacc ggggaaagat cgtaccgctt ggagttcaga aacaaattgt cgaatagcgc
    10381 tcgcgcggtt tcgaccgagg gcgtttctcc cggtcgcaaa cggcgataga tctcgaccat
    10441 ggcctcttcc ttcgagaccg ttcgttccat ttcaagtgtg tcgagaatca ccggcgttga
    10501 ggtcgcagca tcaaagaaga tgactttaaa ttcctcgatt tcactttcaa ggatcttctc
    10561 gatgatctcg gcggtgagac gctggttttt ttccaccagc ttgttcttct tggtatcgac
    10621 cacttcgctc aacaccgccc gaccgaccaa ctcgcttggg gcgacgggga tttctttgac
    10681 gccggccgcc ttgagcttgg cgatgatacc ttttgtcagt ttgccgcctt cccgtaccag
    10741 cggttctttc ccccccttgt cgacgacctc cgtcgagcag cgcatgcctt gatggatttc
    10801 ggggtcaagc ttacgcatga gcttaccttt attgactcgg atttcttcga ctgggtaata
    10861 cattttcagc agatcgtcgc tcgaaaagcc gaatgccttc agcaatatcg ttgctggcat
    10921 ttttcgtcgg cggtcgatgc ggacgtacag aatgtcgcgt gcatcgaatt cgaaatccag
    10981 ccatgaaccg cgatagggaa tgatgcgggc gctgtagagg actttgccgc tggcgtgcgt
    11041 ccggcccttg tcatgcgtga atgacgcacc cggcgaccgg tgcaactggc tcacgacgac
    11101 gcgttctgtt ccattgacga taaacgtgcc gcgttcagtc atcagcggca attcgccgac
    11161 atagacctcc tgttcgcgga cgtcgaggac cttcttcttc gggcctttgt cttccttatc
    11221 gaacaccacg agccgtactc gcagtttgag cggcacggca aaggtcatgc cctgctcgag
    11281 gcattcccgt tcgtcatact ttggcgtccc aagtgtgtaa cttgtgaact ccaggatggc
    11341 tgtattgttg tagtccggaa tcggaaacac gctggccaag gccgcttgca gtccgtgatc
    11401 cttccgccgt tcgggctcga cttcgaattg aagaaactcc tcgtacgaac gcttctgaat
    11461 ctcgatcaaa tccggaatgt cgatgctggt tcgaatacgg gaaaaatcct tccgttcgac
    11521 gaactcttgt agagtcgttt cgggcattcc gcaatcctcc actacatatg gttgggcttg
    11581 ggcacccgtt tgagcggatg cccacagaac tgaacagacc gagcttactt gatctcgact
    11641 tttgcgccgc tctcctcgag cttcttcttc atggcatcgg actcttcctt ggttgccccg
    11701 gtcttgacag gcttaggagc gccttccacg agatccttgg cctccttgag tccgaggttg
    11761 gtgagttcgc gcacgacttt gatgacttga atcttcttgt cggcaggtgc cgacgcgagg
    11821 atcacgtcaa atgccgtttt ttcttctgcc ggagcggcag cggctccccc ggcggcaggc
    11881 gccgcggcaa tggcaaccgg agcagcagct gtgaccccga accgagactc caaacctttt
    11941 accaactcgg ccaaatccag cacgctcatg ctttcaatgg ctttgattaa ttcctcttgc
    12001 gataccttcg ttgcagtggc tgacatatcc ccttctcctt tccgtttatc ctgaatggct
    12061 gcaatgactc tcacaaactt ggttaagacg gcattcaacg tatagaccac gccgcgaatc
    12121 ggaccttgca tggccgacaa cagcatggca atcaacactt cttttttggg cagcttggca
    12181 acagcagcca actcggcggg ctgcaggatc ttgccctcca agacccccaa catgatacgg
    12241 atcttttctt cgcgcttgtc cgccccgata aaatccttga ggatcttcgt gggcaacact
    12301 ggatcgtcat agccgatgac gacgcccgtt ggtcctttta gatgggtctt caacccggcc
    12361 agtgttgttc cctcggatgc ccggatcgcc agggtgttct tcatgacctt gtattcggct
    12421 ttggctccgc gcaattgttt acggagctcg gtcacctggt tgaccgaaag cccggccgat
    12481 tcagtcacaa ttgccagtcg agcacgtccg aatttttccg tcaattccgc aaccgctgtg
    12541 gccttctctt cccttttcat ctcatcctct ttctctgtgg ctaccgcccg acttccaagg
    12601 gatatcggat cggatcaact ccactgcttc gccagggcaa tggcgtccaa cttcaccccg
    12661 atgcccattg tgctcgaaat cgtcacgctc ttgagatagc gtcccttgca ggaagcaggc
    12721 ttcgccttga cgaccgcctc gaggaccgcc gacgcattgt cgaaaagttg atcgggctga
    12781 aatgacactt tcccgactgg gacctgcaca attccggcct tttcgacctt atactcaaca
    12841 cgacccttcc taatctctcc gacggccttg ccgacttcga aggtcacagt tccggttttc
    12901 ggattgggca tgagcccacg gggccccaac tgtttcccaa gcttaccgac tgatgccatc
    12961 aaatccggcg tcgcaatggc ataatcaaaa tccatccagc cgcctttgat cctttccatc
    13021 agatcgtctg atccgacgaa atcggcaccg gcctggcgtg cttcctgttc cttttcccct
    13081 ttggcaaata cgagcaccct gactttcttc cccgtgccat gagggagaga cgtggtcccg
    13141 cgtaccattt gatcggaacg cttaggatca acaccgagcc ggatcgccag atcgactgat
    13201 tcgtcgaatt tcgcgtaggc ggtgcctttg accaatgcaa ccgcgtctcg gagcgcatag
    13261 ggacgcgcct ccattttggc gagtgcggcc ttcattttct tacccatcgc agactctcct
    13321 tgcgttgtcg ttaaccctgg accaccaccc ccatactgcg ggcagttccc tcaatgatct
    13381 tgaccgctcc ttcgagatcg gcggcattca aatcggccat ctttttttgc gcaatctcgg
    13441 tgagctgctt gcgcgtgatt ttcccgacct tgtccttttg gggcacgccc gatcctttga
    13501 tgatgccggc cgcttttttc agcaggtcgg acgcgggcgg agttttcata ataaacgtga
    13561 atgaccggtc cttgtacacg gtgataatca ccgggatgat gctgtcgcct tccttttgcg
    13621 tcttggcatt gaactgcttg caaaactcca tgatgttcac gccatgttga cccaatgaag
    13681 ggccgaccgg aggagccggg tttgccttgc ctgccggaat ctgcaacttg atttgggctg
    13741 atacttcctt tgccatgcac gactctcctt atatacgttc aacctgcagg aaccccaatt
    13801 cgaccggcgt tgagcgccca aaaatgctga ccatcacctt caggcggttg tgatcctggt
    13861 ccacctcgtc cacgacgccg ttgaaaccga ggaatggccc gtccacaatg cgcacattgt
    13921 cggtcttgat gaacttaacc tgttcgcggg gaccagcact cccggcatca acctgtttca
    13981 acaactccgc cacttcctcg ttagtcaacg gcgtcggctg cgcgcctcca ccgacaaacc
    14041 cggtgacctt gggtgtttcc ttgatcagtt gcagcgtttc gtccgtcaca ggcgtttcca
    14101 attcgacgag gacatacccg ggaaagaatt tccgacggga ggtgcggcgc ttgccgtcct
    14161 tgatctcaat cacgtcttca gtcggaacca gaacctgacc catcttttcg gtgagcgcca
    14221 tttgattggc ccgctcaagc aagctcgttt tcacccgccc ctcaaaacct gcataggtat
    14281 gaatgacgta ccagtttttt cccataccca ccttcggatc gtttgttggc gcggcttcgc
    14341 tcatatgatt tttcccacca gccaggataa aaacgagtcg atcacagaga gataaattga
    14401 catcagaacg caaaacacga tgacgacggt cgtcgatccg atcgtctcgg ctttcgttgg
    14461 aaacgagact ttcttgagct ccgttcggac atcgccgagg aactccttca cgttatcgat
    14521 catccgtttg aacatggctc acctaggctg attactgcga tctcatcaca ccacagacac
    14581 catcgaatcc atccctcgcg cccatcgtga gcgctccggg tcgagcaagc ttcttattca
    14641 gacccaaccc ctaaaggtgc tccatccgtc tgccgagaac catctgatgg cagacggtac
    14701 gagcgagctt ggtttggcag gggcactagg atttgaaccc agactctcgg ttttggagac
    14761 cgatgtgctg ccattaacac catgcccctc cggcacagac atccgagcgt ggagtctaca
    14821 gagcttccgg ctcggctcag cgctcagaca aaacacttat tttacttcct tatgaggcgt
    14881 gtgcttgcgg cagaacttgc aaaacttatt tcgctccaat cgatcaggat catttttttt
    14941 gttcttggtg gttgaatagt tcctctgctt acaggccgtg caggccaaat cgatgatctc
    15001 tcgcatctct atctcctgtc ctctgctgcc ctaggccaga atttccgtca cgacgccgga
    15061 gccgaccgtc ttgccgccct cgcgcacggc aaagcgcaac ccctggtcca tcgcgatcgg
    15121 gctgatcaat tcagccgtca cgctcacgtt gtcgcccggc atcaccatct ccacccctgg
    15181 attcaacgac accacgcccg tcacgtcggt ggtccggaaa tagaactgcg gccgatac
//
LOCUS       DASSCQ010000050        10241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_919411, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000050 DASSCQ010000000
VERSION     DASSCQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919411"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            518..2122
                     /locus_tag="VFH05_03690"
     CDS             518..2122
                     /locus_tag="VFH05_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET6677342.1"
                     /translation="MHTSVTDPAQSRLRGWQFALFNVVMGLAHMLVLFNAGSYVALLP
                     HAAGDLGGVLPSFGTWAQTDFMIALALAFPIGRWLSGRYGESRVFAGAFLVYAAASAL
                     CAIDHTISAFVPARIVLGLAGGLTLPLGQTLLLEEYPDRLKSLGLAIWGLFTLMPFTA
                     GLALGGWLADEWNWRMLFYVNVPLALAIAGFTGSLLHGRPIRTRDERFDFIGFLLLAV
                     IFMGLQTILNEGNDFDWFDDPWLRALLLLVIATVPIWIVWELGERRPAVDLRLFRHRN
                     FVIGLICLALGFMSVQGLLALFVVQLQVLMGYSSSLAGMVFVPMVVCGVPLIAVMHEV
                     AKRLDARLLASLNAVGFAATFYWIGLFDDPQSYDQIFWPMVVEGIFLGSFFTPLTVLT
                     LHGLSGDQMLRAAEVANVFRLAAGALGITWQGVIVFRRLPFHHMQLSDHFGGRISASY
                     DAFQQLTLKLQEIGFDPSAIQRKLHLLIKQQAGILALNDAFLLSSYLCLGLALLVWCA
                     HSSRVPVMKPAEELRERRAEELMEQP"
     gene            2119..3627
                     /locus_tag="VFH05_03695"
     CDS             2119..3627
                     /locus_tag="VFH05_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET6677343.1"
                     /translation="MTSIVRFTRQSGGALLVSTLALLSGCAWIPKGDPPATYLEPPEM
                     KETLAEVTSRLEQWPDDHWWEQFNNPELNQLMEAALKDNPGLKMASARLREAQGFVRV
                     EGARLLPFLEADASLTYERISQHGVFAALNPEVAGARILLGVINPLSLRYEFDFWGKN
                     RAMLEAALGQAAAEEAELAQVRLHLTTGIARAYVRGHALYRQLELVRAIVDLRRKLRQ
                     LAETRFQLGLDNDLAVKQAIADYETAVKRQAGVRDQLDVQRHLLARLTGKGPDEGLRL
                     FNRPGSALPDSLPLPEHLPMGLLVHRPDLAATLYKADSAARLVKVAKTQFYPTIDLTA
                     FVGFNALTLTKGADQLANFLFNGQSFSYGIAPGIRLPLFEGGRLRAELSVQRAEYDAA
                     VELYNETLLSALREVADSLSAWQATREMLESHRRLVASLTDDWRLAKVRLVTGLDDDR
                     EVLRHRYPVLEQEYSLRALESDQLVAAVDLIEALGGGYHNPDIAKRPATQPS"
     gene            3632..5149
                     /locus_tag="VFH05_03700"
     CDS             3632..5149
                     /locus_tag="VFH05_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET6677344.1"
                     /translation="MSTTHSLPPPAPHMYEESLHVRRNRRLLLVAGALMLAAVAYSLH
                     WWTSARFWVQTDNAYVTGNLVPVTAQATGIITHVLFEETQFVNRGDVMIRLDEHEAYA
                     ALGRARGRLGETVRRINALFLTQRQLGEKLAARRARLGIIRHDVDRYRQASPRGAVSK
                     QILQNATDQLEALEADVRETQAEYDALDAQIGGTTVMEHPAVELAKHEFINAHLEYAR
                     QLIRAPVSGYIAKRKSQVGDRVKPGTNLMTIVPLDHLWVEANLRETEVGEIRPGQPAE
                     VRIDLYGGKHTFHGTVEGLVPGTGSPFALLPPDNSTGNFIHITERVPVRIALAADEIR
                     DYPVRPGLSTVTRIRISEPGNSVWSSLAKADTEEYQTDVYGDELTHAESMAQGVIAGN
                     VRANEHEGHLGRHASQTGQLAGSGGQDHQRLSTPSETRQSKDGVFGEPRPFGRPLEPE
                     SYVPPRSPDLGSTYFPLSPAIGPGSNGLGPEAGPPPPRREPFDLRERSGARHRDR"
     gene            5219..7153
                     /locus_tag="VFH05_03705"
     CDS             5219..7153
                     /locus_tag="VFH05_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677345.1"
                     /translation="MKGRLVRLFNNLPIARKLLLTALIPLAAFLVLGIQTYLSLQTLT
                     EHEEQLDRVYVAQRAAAEYMRLIVDYETGFRGYVMTRQDAYLIPSRNAHQHLPSVEQV
                     LLGLVRPHRAQYETVQAVQRLVTRLMEEKGSLIQSVKKGETAEALRYIEQGRGQALMQ
                     IVREHMAHFDRLEQEALNDALANMGRSRNSMVARMLWGIALVFVLMVLALQLIARSIT
                     GPLIGLVKSVRSSDGLVPTDVPVLSRQDELGELTRVMGAMSEQVRKHLASVQANEAEL
                     RALNEDLAASEQKYRSIVDHAPFGIFTTSGMTVNFSNRYNRMLAGLNPDDTSEPEAFR
                     EWIHPDDRDRVLHEYAQAVAEDRPYETVFRFLHRDGSLRKVLSRRIPIRNKEGQTTMY
                     QGFNVDITALDAMQQQLSRAERLATLGQVAAGIAHEIRNPLVGIGSTAALLRDEFDSS
                     DEKRADLDIILNETRRLDRIVNQIIDYARPRDLMPTTWPVEGFVDEVLKLLDTRLEGQ
                     RIKIVRQCEPDLYACADRDQIKQVLLNLCHNSLDAMPTGGELRMSAGQAARVGTPGVY
                     IEVTDTGSGISQKDLAQVFQPFFTSGKQHGTGLGLAICRNIAEAHGGDMVLTSEPGRG
                     TTARLWLPLQPARDYAEFSA"
     gene            7150..8541
                     /locus_tag="VFH05_03710"
     CDS             7150..8541
                     /locus_tag="VFH05_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6677346.1"
                     /translation="MNATIFVTDDEPAIRAALVKRLSRRQHRVVAFESGEVLLAALDH
                     EVPDLMLLDVKMPGLSGLETLKVCRAKAPFALVIMLTAYGTVQDAVEAMKLGAYDFVI
                     KTVDLDGMEQVLDRALELFALRRRLSYQSEQERDQYAIDHLIADSAAMKSLVEQIREV
                     AHNPKSTVLLLGETGTGKEFVARVLHHNGSRASGPFIGVNCTAIPRDLFESELFGYER
                     GAFTGAHQRKLGLLDRAEGGTLFLDEIGDLDLTMQAKLLRVLQERTFRRLGGTTDIAA
                     DFRLITATNRDLKKAITTGTFREDLFFRLNVVALELPPLRQRIEDIFPLSMKALLHYG
                     TEFAKDVAEIDAEARGLLEAYHYPGNIRELQNIIERATIFCTGRTLTANYLPRELRDQ
                     AKHTATAVPHGDGQVIRVEMQVGTHTLSEIEDSIIEETLRLADYNKSLAAKQLGLTRF
                     ALDRRLKKIKKDD"
     gene            complement(8564..8881)
                     /locus_tag="VFH05_03715"
     CDS             complement(8564..8881)
                     /locus_tag="VFH05_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6677347.1"
                     /translation="MKVINLSTFQVFSAEKMKKNNIFQTPRFFCDVYCFEPGQEQIGH
                     VHGEQDKVYLVLEGNGTFQVGTDKQVLGPGQGTMAPAGEEHGVKNDSGQRLKVLVFVA
                     PNP"
     gene            8979..>10241
                     /locus_tag="VFH05_03720"
     CDS             8979..>10241
                     /locus_tag="VFH05_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677348.1"
                     /translation="MNRADRRRQGASRKTGDQSVRAWLDQGRAHQQAGRLTEAEDSYR
                     HALELAPHDAESCHLLGLVTYRLNRLAESLRYLHAAVERQPSLPVYWFNLGVVSQKAG
                     RSPDAVSAYEKAIALNPRYPDAYGNLGNVLREIGDLPGAVNAYERALKLNPSHADTHN
                     NLGVAKKEQGHTRDAIVCYRRALELRPTHAEALNNLGLALMEIGTLHDAIGCFQQALS
                     VLPGYQLARYNLGMAWSWAGDDSKACECLEMVARARHDHRSLPADTAIYRSRIKHDAE
                     QAQFLFEQGKLDEQYRPYAQSLRRLQERLESDSRPGNRLMIEPSELIAIAPSFNRILN
                     CEPPPRIGHGALNPLLDVLAVEAQYHATRPEVTYIDNLLTQPALDALRRFCWEATIWK
                     KDYENGYSGAFLGDGFASPLLFQIADELR"
BASE COUNT         2103 a         3079 c         2923 g         2136 t
ORIGIN      
        1 gacgaacaaa tccagccgtt cgggacgttc caatacgtca atctcgataa gccggccccc
       61 atcaagggca tcgatgtcaa cgcgccgaac cactcggtta ataataatct gacgaaccct
      121 ttccttccct gagcgaagag cgctccaaac caaagttttc actttcccac tcgattgcct
      181 ggcagaagtt ctcgagccac ggcgtctgat cacatcgttg agtgaagtgt tttcggctga
      241 gcgaatccgc acctaccgct caggtcgaat tcaaacatgc acgctgaagc catgcagtcg
      301 tcgtatccct attttgcaca tgtcaacaca ggttccgcgg ctaagcgggc ggatgggaat
      361 acggcgtcct ggtccagaag ttgctgtcgt cacctacgct ggcctcgaag cgccgcgaag
      421 catcgacttc aacgagaggt tgctaggccc cgaccggaac cgtctctgac ccagcggttg
      481 gcgaccatcc cccttttggc atgtatttga gcatcgtatg catacctcag ttactgatcc
      541 cgcacagagc cgcctgagag gatggcagtt cgccctgttc aatgtcgtga tgggcctcgc
      601 ccatatgctg gtgctcttca acgcgggctc ctatgtcgcg ctcctgccgc atgcggcggg
      661 ggacctgggc ggggtcctgc ccagcttcgg aacatgggcg caaacggatt tcatgattgc
      721 cctggccttg gcgtttccta tcggacgatg gctctccggt cgctacggcg aatcccgggt
      781 cttcgcgggc gccttcctcg tgtatgcagc ggcctcggct ctttgcgcca tcgaccatac
      841 tattagcgcc ttcgttcccg ctcgcatcgt gctgggcctg gctggcggtc tcacattgcc
      901 gctgggtcaa acactgctgt tggaagaata tcccgatcga ctcaagtcgc tggggctggc
      961 tatctgggga ctctttacgc tgatgccgtt cacagcggga ttagcattgg gtggctggct
     1021 ggctgacgaa tggaactggc gaatgttgtt ttatgtcaac gttccactgg ccttggccat
     1081 cgccggcttc accggctctc ttctgcacgg gcgtccgatc cggacgcgag acgagcgctt
     1141 cgatttcatc ggatttctct tattggcggt catcttcatg ggactccaga ccatcttgaa
     1201 cgaaggtaac gactttgatt ggttcgatga tccctggttg cgtgcgctct tgttgctcgt
     1261 catcgcgact gtgcccattt ggatcgtgtg ggagctggga gagcgccggc cggcagtgga
     1321 cctgcgcctg tttcggcatc gaaattttgt gattggcctc atctgccttg cgctcggctt
     1381 catgtcggtc cagggactgc tcgcgctctt tgtcgtccag cttcaagtct tgatgggata
     1441 ttcttcgtcg ctggccggca tggtcttcgt gcccatggtc gtctgtgggg tgccgctgat
     1501 tgcggtcatg cacgaagtcg ccaaacggct cgatgcccgt ttgctcgcca gcctcaatgc
     1561 ggtcggtttt gcagccacct tttactggat cggactcttc gatgacccgc agtcctatga
     1621 tcagattttt tggccaatgg tcgtcgaagg catcttcctc gggtctttct tcacgcccct
     1681 cacggtcttg acgctgcatg gtctatccgg tgatcagatg ctgcgtgcgg ctgaagtggc
     1741 gaatgtcttc cggctggcgg ccggcgcact cggcatcacg tggcagggcg ttatcgtgtt
     1801 tcgccggcta cccttccatc acatgcaact cagcgaccat tttggcggac gaatatccgc
     1861 ctcctacgac gcgttccaac agctgacctt gaagttgcag gagatcggct tcgatccgtc
     1921 cgcgatccag cgtaaattgc acctgctgat caaacaacag gccggaattt tagccctgaa
     1981 tgacgccttt ctcttgtcga gttatctctg tctcggactc gcccttctcg tctggtgtgc
     2041 ccattccagc cgtgtgccgg tcatgaaacc ggccgaagaa ctgcgagaac ggcgagcgga
     2101 agaactcatg gagcaaccgt gacgagcatc gttcgattca cacgccagag cggaggagca
     2161 ctgctggtct cgacactggc actgctgtcc gggtgtgcct ggattcccaa gggagatccc
     2221 cccgcgacct acctcgagcc gcctgagatg aaagagacgc tcgccgaggt caccagtcgg
     2281 ctggaacaat ggcccgatga tcactggtgg gaacagttca acaatccgga gctgaaccaa
     2341 ttgatggaag cggcgctcaa ggacaatccc ggcctcaaaa tggcatcggc ccggttgcgc
     2401 gaggctcaag ggttcgtgcg cgtcgaaggc gcgcggcttc tgccattctt ggaagccgat
     2461 gcctcgctca cgtacgaacg gatttctcag cacggcgtgt tcgcggcgct caatccggaa
     2521 gtggcaggtg cgcgcatcct cctgggcgtg atcaaccccc tgagcttgcg ctacgagttc
     2581 gatttttggg ggaagaatcg ggccatgcta gaagcggcgc tcggacaagc ggccgccgaa
     2641 gaagcagagc tggcacaggt ccgcctgcac ctgacaaccg gcatcgcgcg tgcgtatgtc
     2701 cgcggccatg cgctgtaccg gcagctggag ctggtccgcg cgatcgtcga tctgcggcgc
     2761 aagcttcggc agctcgccga gacacgcttt cagctgggct tggacaatga cctggcggtc
     2821 aaacaagcca tcgcggatta tgaaaccgcg gtgaaacggc aggccggcgt gcgcgatcaa
     2881 ctggatgtcc agcgccactt gctggccaga ttaaccggca aaggcccgga cgaaggtctc
     2941 cgcctcttca accggcccgg gtctgctcta cctgactcac tgccactacc tgaacacctc
     3001 cccatgggat tgctcgtgca ccgtcctgat ctggccgcga ctctctataa agcggattcg
     3061 gcggcgcggc tcgtcaaagt ggccaagaca caattctatc ctacgatcga tctgaccgct
     3121 tttgtcggct tcaatgcgct gaccttgacg aaaggcgccg atcaactggc gaatttcctc
     3181 ttcaacggtc aaagtttttc ctatggcatc gcgccaggca tccgtctgcc gttgtttgaa
     3241 ggtggacgct tacgcgcgga actttctgtg caacgggcgg aatacgacgc cgccgttgaa
     3301 ctctacaacg agaccttgtt gagcgcgtta cgtgaggtcg cggatagtct cagcgcctgg
     3361 caagccacca gagaaatgct cgagtcccac cgccgactcg tcgcatcgct gaccgacgac
     3421 tggcgtttgg ccaaggtccg actcgtgacc ggactcgacg acgatcgtga agttctccga
     3481 catcgctacc cggtgcttga gcaggaatac tcactccgag cgttggaaag cgatcaattg
     3541 gtggccgctg tcgatctcat cgaagcactc gggggcggct atcacaatcc cgacattgca
     3601 aagagaccgg ccacacaacc gagctgagac aatgagcacg acgcattccc tccctcctcc
     3661 ggcacctcac atgtatgaag agtcgctgca tgtgagacgc aatcggcgac tactgctcgt
     3721 cgccggcgct ttgatgcttg ccgcagtggc ctatagcctc cactggtgga catccgcgcg
     3781 gttctgggtg caaaccgaca acgcctatgt gacaggcaac ctcgtccccg tgacggctca
     3841 ggcaaccggc atcatcactc acgtcctgtt cgaggagacc cagttcgtca atcggggaga
     3901 cgtgatgatc cggctcgatg agcatgaggc ctacgccgcg ctcgggcggg cgcgcgggcg
     3961 gcttggggaa accgttcgcc gcatcaacgc gctcttcttg acacagcgac agctcggaga
     4021 aaaactcgcg gctcggcggg cgagattggg catcattcgt cacgacgtgg atcgctaccg
     4081 tcaggcctct ccaagggggg ccgtctcaaa gcagattttg cagaacgcga cggatcagct
     4141 cgaggcactg gaggccgacg tccgggaaac tcaggccgag tacgatgcgc tcgacgccca
     4201 aatcggaggc acgacggtca tggaacaccc ggccgtcgaa ctggccaagc atgaattcat
     4261 caatgcgcat ctcgaatacg cacggcaact gattcgtgcg ccggtctccg gctatattgc
     4321 caagcgcaaa tcgcaagtgg gggatcgggt caagcccggc acgaatctca tgaccattgt
     4381 cccgctggat cacctgtggg tcgaagccaa tttacgagaa actgaagtcg gcgagatccg
     4441 ccccggccag ccggctgaag tgcgcatcga tctctacggc ggcaagcaca catttcatgg
     4501 caccgtggaa ggtctcgtgc ctggaaccgg cagcccgttc gcgctgctgc ctccggataa
     4561 ctcgacgggc aacttcattc acatcaccga acgggttccg gtccgcattg ccttggcggc
     4621 ggatgaaatc cgcgactatc ccgttcggcc cggactgtcg actgtcacca ggatccggat
     4681 aagcgagcca ggaaattcgg tctggtcatc gctcgcgaaa gccgatacgg aggaatacca
     4741 gaccgatgtg tacggcgacg aactcaccca cgccgaatcg atggcacagg gcgtcatcgc
     4801 ggggaacgtg cgagccaatg agcatgaagg gcatctgggg cgacacgctt cgcagacggg
     4861 tcaacttgca ggaagcggcg gacaagacca tcagcgtctt tccacgcctt cagagacccg
     4921 ccagtcgaaa gacggggtgt ttggcgaacc gcgtcccttt gggcgccctc tcgaacccga
     4981 atcctacgtg ccgccgcgga gtcctgatct tgggtctacc tattttcccc tgtcccccgc
     5041 aatcggaccc ggttcgaatg ggttggggcc tgaagccgga ccgccgcctc ctcggcgcga
     5101 gcccttcgat ctgagggaac ggagtggggc acgccaccgc gatcgataag catgcgcctc
     5161 ctgattgaca tggcttctct gctcttctta cgatgaacgc aaggagcaag acggcctcat
     5221 gaagggacga ttggttcggt tgttcaacaa cctgcccatt gcgcgcaagt tattgctcac
     5281 ggcgctgatt cccttggcgg cgttcttggt tcttggcatc caaacctacc tcagcctgca
     5341 aactctcacc gagcatgaag agcaattgga tcgcgtgtac gtcgcgcaac gtgcggcggc
     5401 agaatatatg cgcctgatcg tcgactatga aaccggcttc cgaggctatg tcatgactcg
     5461 acaagacgcc tatctcattc cttcgcgaaa tgcccatcaa caccttcctt ccgtcgagca
     5521 ggtgctcctg ggcctcgttc gcccgcatcg cgcgcagtat gaaacggttc aggccgtgca
     5581 acgcctggtc acgcgattga tggaggaaaa gggaagcctt attcagtccg taaagaaggg
     5641 agagacagcg gaagccctgc ggtatatcga gcaaggccgc ggccaagccc tcatgcaaat
     5701 cgtgcgcgag cacatggcgc atttcgaccg actggagcag gaagccctca acgatgcgct
     5761 tgccaatatg ggccgctccc ggaattcgat ggtcgctcgc atgctctggg gcatcgcgct
     5821 ggtgttcgtc ctcatggtgc tcgcgctaca gctcattgcc cgctcgatta ccggaccgtt
     5881 gatcgggctg gtcaaatctg tccgttcgtc ggacggactc gtgcccacgg acgttccagt
     5941 cctctcgcgg caggacgaac tgggagaact cacgcgggtc atgggcgcga tgagtgagca
     6001 agtgcggaaa cacctcgcca gcgtgcaagc taatgaggcg gagttgcgtg ctttgaatga
     6061 agacctcgcc gcctccgaac aaaaataccg cagtatcgtc gaccacgcgc ccttcggaat
     6121 ttttaccact tcgggcatga cggtcaactt cagcaatcgc tataaccgga tgctcgccgg
     6181 gttaaatcct gatgacacca gcgaaccgga ggccttcagg gagtggatcc atccagacga
     6241 tcgggatcgt gtcctgcacg aatacgcgca agccgttgct gaagaccgcc cctacgagac
     6301 ggtgttccgc tttcttcacc gcgacggatc gctcagaaaa gtcctgagcc gccgtatccc
     6361 aatccgcaac aaagagggcc agaccaccat gtatcagggg ttcaatgtcg acattacggc
     6421 cctcgacgcc atgcagcagc aattgagccg tgccgagcgc ctggcgacgc tcggtcaggt
     6481 cgccgcgggc attgcgcatg aaatccggaa cccgctggtg ggcatcgggt caacggccgc
     6541 cctgctgcgg gacgagttcg actcttccga tgaaaagcgc gcagatctcg acatcatctt
     6601 gaacgaaacg cgcaggctcg accgcatcgt gaaccagatc atcgactacg cccgtccgcg
     6661 tgatctcatg ccgacaacct ggccggtgga aggattcgtg gatgaggtat taaagttatt
     6721 ggatacacgt ctggaaggtc aacggatcaa gatcgttcga cagtgtgagc cagacctgta
     6781 tgcctgtgcg gatcgggacc agatcaaaca ggtattgcta aatctctgtc acaattcatt
     6841 ggatgccatg ccgaccggcg gtgaactgcg catgagcgcc ggacaagcgg cgcgcgtcgg
     6901 aactcccggc gtctacatcg aggtgaccga cacgggctca ggcatcagtc agaaagacct
     6961 cgctcaggtg tttcagccct tttttacatc cggcaaacag catgggaccg gcctgggcct
     7021 ggccatctgc cggaacattg ccgaagcgca cggaggggac atggtcttga cgagcgagcc
     7081 cgggcgcgga accactgcgc ggctctggct gccgctgcag ccggcgcgcg actacgcgga
     7141 gttctccgca tgaacgccac cattttcgtc accgacgatg agcccgcgat ccgcgcagcc
     7201 ctcgtgaaac gtctgtcacg gcgacaacat cgcgttgtgg cgttcgaatc cggcgaagtt
     7261 ttgctcgcag ccttggatca cgaagtgccg gacctgatgc tcttggatgt caagatgccg
     7321 ggtctctccg gtcttgaaac gctcaaagtc tgccgcgcca aagcgccgtt tgcactcgtc
     7381 atcatgctca cggcctatgg gacggtgcag gacgccgtgg aagccatgaa gctcggcgcc
     7441 tacgatttcg tcatcaaaac cgtggatctc gacgggatgg aacaggtcct ggaccgggcg
     7501 ctggagctgt tcgcgctccg acgccgtctg tcctatcaaa gcgaacaaga gagggatcaa
     7561 tatgcgatag accatctcat cgccgacagt gcggcgatga aaagtcttgt tgagcaaatt
     7621 cgagaagtgg cgcacaatcc caagagtact gtcctcttgt tgggagagac cggcacgggc
     7681 aaggagtttg tcgcccgtgt actgcaccat aacggcagcc gcgcctccgg gccgttcatc
     7741 ggcgtgaatt gtaccgccat cccccgcgat ctcttcgaga gcgagttgtt cggctatgag
     7801 cgcggcgcct tcacgggagc gcaccagcgc aagcttggcc tgctcgaccg ggcggaaggc
     7861 gggaccctct tcctcgatga aatcggcgat ttagatctga ccatgcaggc caaattgctg
     7921 cgagtgctcc aggagcgcac cttccggcgg ctcggcggca ccaccgacat cgccgcagat
     7981 ttccgattga tcaccgcgac gaaccgcgac cttaaaaaag ccatcacgac aggcaccttt
     8041 cgggaagacc tgtttttccg gctgaacgtc gtcgcactcg aattgccgcc gctgagacag
     8101 cggatcgagg acattttccc cctgagcatg aaggccttgt tgcattatgg gacggagttt
     8161 gccaaggacg tggccgaaat cgatgcggag gcgcgggggt tgttagaagc ctatcactat
     8221 ccaggcaaca ttcgcgagct gcagaatatc atcgagcggg cgacgatttt ttgtacggga
     8281 cgaacgctca ctgccaacta tcttccccgt gaattgcgag atcaggccaa acatacagcg
     8341 acagccgtcc cacatgggga tgggcaagtg atcagggtcg agatgcaggt cggcacgcat
     8401 accctgagcg agatcgaaga cagcatcatt gaagagaccc tgagacttgc cgattacaat
     8461 aagagccttg cggcgaaaca gctggggctg accagattcg cgcttgaccg ccgcttaaaa
     8521 aagatcaaaa aagatgatta gcgccagact ggttacgaca acatcaggga ttcggtgcca
     8581 caaagaccaa gacttttaat cgctgtccgc tgtcgttttt caccccgtgt tcttcgccgg
     8641 cgggagccat cgtgccttga cctggcccca gcacttgctt atcggtacca acctgaaacg
     8701 tcccgttccc ttccaagacg aggtagactt tgtcctgctc gccatgcacg tgcccgatct
     8761 gttcctgccc cggttcgaaa caatagacat cgcagaagaa ccgcggcgtt tgaaagatat
     8821 tgtttttctt cattttctct gcactaaaca cttgaaaagt tgaaagatta atcaccttca
     8881 tgctcacgct gcctcccctt gttagacaga gagttcgcac cctcctttga cgctcgcggg
     8941 ctttttcggc tactgtcgat accccggttg aatgcatcat gaatcgagca gatcgacgcc
     9001 gtcaaggcgc atcacggaag acaggagatc aatcggtccg ggcctggctc gatcaaggcc
     9061 gggctcatca acaggccggt cgacttaccg aggcggaaga ctcctaccga cacgcgctcg
     9121 agctggctcc gcatgatgcc gagtcctgcc atctcctggg cctggtcacc taccgtttga
     9181 atcgacttgc tgagtccctc cgctacctcc atgcggcagt cgaacgacaa ccttctttgc
     9241 ctgtctactg gttcaatctc ggtgtggtat cgcaaaaagc cgggcgttcg ccggatgcgg
     9301 tatccgctta tgaaaaagcc atcgccctca atccccgtta tcccgacgcc tatgggaact
     9361 tgggaaatgt tcttcgcgag atcggagatc taccgggggc agtgaacgcg tatgagcgtg
     9421 ccttgaagct caatccgtcc catgccgaca cgcataacaa cctgggggtt gccaaaaaag
     9481 agcaaggcca caccagggat gccattgtct gctaccgccg cgccctcgaa ttgaggccga
     9541 cccatgctga agcgttgaac aatctcggct tggcgctcat ggaaatcggc acgcttcacg
     9601 atgcgatcgg gtgctttcag caagccctct ccgtcctccc cggctatcaa ttagcccgat
     9661 acaatttggg catggcctgg tcatgggcgg gagacgactc gaaagcctgc gaatgcctcg
     9721 agatggtcgc gcgggccagg catgatcacc gttcattacc ggctgacaca gccatctatc
     9781 ggtcgcggat caagcatgat gccgagcagg cgcagttctt gttcgaacaa ggcaagctgg
     9841 atgagcagta tcgtccctat gcacagtcct tgcgccggct gcaagaacgg ttggaaagcg
     9901 attcacgccc tggaaaccgt ctcatgattg agccgagtga actcatcgcc atcgcgccat
     9961 catttaatcg catcttgaat tgtgaaccgc ctcccagaat tggccatggc gcattaaatc
    10021 cattgctcga tgtccttgcg gtggaagcgc aataccacgc tacgcgtcct gaggtgacct
    10081 atatcgacaa cctcttgacc cagcccgccc tcgacgcatt gcgccggttt tgttgggaag
    10141 caacgatctg gaagaaagat tatgagaatg gctacagcgg cgcgttcctt ggcgacggat
    10201 tcgcctcacc cttgcttttt cagattgcgg acgaactgcg t
//
LOCUS       DASSCQ010000051         7848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_480290, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000051 DASSCQ010000000
VERSION     DASSCQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480290"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VFH05_03725"
     CDS             complement(<1..111)
                     /locus_tag="VFH05_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET6677349.1"
                     /translation="MQKTRIICTIGPATESYEMLQKMYEAGMSIARLNMSH"
     gene            441..911
                     /locus_tag="VFH05_03730"
     CDS             441..911
                     /locus_tag="VFH05_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677350.1"
                     /translation="MIGLVAGMIWLAVGLTSCTGDSSDSPPPAAPETVAGIDANGDGV
                     RDDIEVYIDATYPVSTQGDTNRALRQFARAAQSTMIDASEPAPSVAHAAERFRALECL
                     MARRPGDFHLPFVELRARLLNTAARSFAYLQADNHVRAGNVPLLPADQWSGACL"
     gene            908..1780
                     /locus_tag="VFH05_03735"
     CDS             908..1780
                     /locus_tag="VFH05_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677351.1"
                     /translation="MTRTGMALALTVAWIVAASSMDRAHAQIAAPPACQNVMAPRTTV
                     FFVNGVTTTLDDARLNAGKLELEFLNRLPAMSAAVQANCHVFALNYNPTGGEVNDFFE
                     AAQQQLGITPTRFWLELEGLNLFTRELIRDALEGPLTDLNRIDASTIERHAVAYREQI
                     TSPSCRRVLIVPHSQGNLYTNAAYDLLFNQPPSPPPGTIKIVGVATPAQTVAGNGLYR
                     TSTTDVLINAIRLIRPGTLPPNTNWGITPLLVSPSYSGGHSFIGYLSADPSRTDLLSD
                     IESSLTALAAVNPC"
     gene            1903..2448
                     /locus_tag="VFH05_03740"
     CDS             1903..2448
                     /locus_tag="VFH05_03740"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HET6677352.1"
                     /translation="MRKTLSVLGSCFVFAIAAAISQAQEVPPASPAAPEAAAKATPAD
                     GHVIHVTAPHLTDGKIRGPFHHYCKIVSPEPFIECLLYETEDNNAKLVGVEYIVAKTV
                     TRDEKVLPKKVWKKVWHDHSVEGVIGNIKILDMSADKAKEFADMVAQTDGVIFSLWPH
                     GAKLPTGKVSIGTMVGHAVHQ"
     gene            complement(2549..3226)
                     /locus_tag="VFH05_03745"
     CDS             complement(2549..3226)
                     /locus_tag="VFH05_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET6677353.1"
                     /translation="MDQIVSKNQRSIGPGIANLIYQRLLTIWSVMISSSKTLKRMMSL
                     GGAFVQRRIDLFEKLPKTTTGLRAFFLSCAAFLFVVDGGATEFSTLVVSVIDGDTIEV
                     LQYQKPERIRLNGIDCPEKGQTFGQRAKQATSDLVFGKDVMLQTHGKDRHGHTIADVF
                     LPDGRKVNQVLVKNGWCWWNRKYAPRDVILEELERRARADRSGLWTDPHPLPPWLYRE
                     LTASANP"
     gene            4257..4646
                     /locus_tag="VFH05_03750"
     CDS             4257..4646
                     /locus_tag="VFH05_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677354.1"
                     /translation="MNILLGLFGNRAKARRAKGALIRAHIASPNIVLYDRSAADSSRE
                     AEQSLWESLKDSSAFAEDRSFYEEGMRRGGTVISVRVNDEQMDQAAEILSREGAIEAA
                     IPEAQEKLQGGQRQVVRGTVRVYKRAA"
     gene            complement(4773..5558)
                     /locus_tag="VFH05_03755"
     CDS             complement(4773..5558)
                     /locus_tag="VFH05_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677355.1"
                     /translation="MLVHIIADYGMGDLAFAEVVQRIKVHLPDTEPVLTPVPAFSTIA
                     AGFCIAQIGLHPSPPGTLIYHNVAPREDDPAARAQNAGERLAYARLPTGVRVIGVNAG
                     YALSFIKDVAEKLRWAASSAEGSQFRSRDVFPQAAAAIALGLPAALGHDLDPESIPSP
                     PLSAVAYVDGYGNLKTTIAYDPNRVRPGKRITVRINERQHEATVSDGAFAVPHGELAF
                     APGSSGWTLRDGSTVRWIELFLRGGSAWNLFDRPAPGSFVAIL"
     gene            5959..6354
                     /locus_tag="VFH05_03760"
     CDS             5959..6354
                     /locus_tag="VFH05_03760"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HET6677356.1"
                     /translation="MHFESGVLKVDARGEFSLEEAKRAFLEMLEAVAQYQAAKVLFDG
                     RNLKGEPASIERFYYGYFAATETIHLIGMKHMRQAPQFAYVMNEPLRDPQRYGETVAV
                     NRGMNVKTFETPEEAFEWLERPPANNPDG"
     gene            complement(6509..7471)
                     /locus_tag="VFH05_03765"
     CDS             complement(6509..7471)
                     /locus_tag="VFH05_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET6677357.1"
                     /translation="MITARQGLILAGSVAMLAMAAISDAKTVKVDMTAVEAEVVIDGE
                     GTTYQAWTFNNQYPGPVIRVQQGDRVDFSFTNTHTSSRPHSMDFHAAQTDFLTHYREL
                     QPGESLQYTWEAKEPGIFVYHCGASPMIQHVARGMIGAIIVDPKNRKAMPKADREYVL
                     VQSELFKDPDDVQAMFDRKYEHVVFNGGVFRYDPVHGKSGGDFLEAKPGERVRIYFVN
                     AGPNNFSAVHPIAELWEDVWESGNPANRLRGVQTVMVPPAGAATMDIVLDKGEGVIPI
                     VTHSLTDALRGAIAILKVHKEAKTLPLMPFMVTGKVKTASLEGK"
     gene            7594..>7848
                     /locus_tag="VFH05_03770"
     CDS             7594..>7848
                     /locus_tag="VFH05_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6677358.1"
                     /translation="MTIKALHHVSVVVDDLAAAIAFFTALGMTIEGERPVEGPWVDRV
                     NGLEGVRVDIVLMRTPDGHGRLELTRFRNPKLVEMEPAIAP"
BASE COUNT         1767 a         2128 c         2224 g         1729 t
ORIGIN      
        1 gtgggacata ttcagtcgag cgatgctcat cccggcctca tacatcttct gcaacatctc
       61 gtaggattcc gtggccgggc cgatcgtaca gataatgcgg gtcttttgca tgcgtgctcc
      121 ctggaatagt ggcgtatcct cagaggtgta gccctcagac ggatgacgac ttgatacggg
      181 aggatacaaa ccgaggccat tctactttta atcgtcggcc gggtctatcg gtatgtgaga
      241 ggtgtttaag atggagagaa aggcgagtga agcgcggtgt gaggtgcgat gagccggccg
      301 catttggaac gtctccggga agatatgact aatggcaact ccgtcagcga gccaccgaca
      361 ggtcgccggt ggaaccatgc ggtataatca acgacgaaaa gtttatgaca tggaacagta
      421 acggcaggaa tgaaggtgca atgatcggtt tggtggccgg catgatatgg cttgccgtcg
      481 ggctcacctc ctgcactggc gactcgtccg acagtcctcc gccggctgcg ccggagaccg
      541 tcgccggtat tgatgctaat ggagatggtg tccgtgacga catcgaagtg tatatcgatg
      601 cgacgtatcc cgtttcgact caaggcgaca caaaccgggc gctccgacaa tttgcccggg
      661 cagcgcaatc gaccatgatt gatgcgagtg agcctgcgcc ctcggtggcg catgcggccg
      721 agcgttttcg cgcattggag tgcttgatgg cacgccggcc tggcgatttt catctcccct
      781 tcgttgaact gcgcgcccgc ctgctgaata ccgcggcccg ttcgttcgcg tatctccagg
      841 cggataatca tgtcagggct gggaatgttc cgctactgcc ggccgatcaa tggagcggcg
      901 cttgtctatg actcggacag ggatggcgct cgctttaacc gtagcatgga tagtcgcggc
      961 gtcgtcgatg gatcgggcgc atgcccagat cgcagctcca ccggcctgcc aaaatgtgat
     1021 ggcaccgaga acgacggtgt tcttcgtcaa tggcgtcacg acgacgctcg acgacgcccg
     1081 gttgaacgca ggcaagctcg agttggaatt cttgaaccgg ctgccggcca tgtcggcggc
     1141 cgtccaggcg aactgccacg tatttgcgtt gaactacaat ccgaccggcg gcgaggtgaa
     1201 cgatttcttt gaggcggcgc agcagcaact gggcatcacg ccaacgagat tttggctgga
     1261 actggaagga ctgaaccttt tcacgcggga actcatccgc gacgccttgg agggtccgtt
     1321 gactgatctc aaccggatcg atgcctcgac catcgagcgc catgccgtgg cgtatcgcga
     1381 gcagattacg tctccgtcct gccgccgcgt gctcatcgtg ccccactctc agggcaacct
     1441 ttataccaat gccgcctacg acctgctgtt caatcagccg ccgagtccgc cccctggcac
     1501 aataaaaatc gtcggtgtgg cgacgccggc ccagacggtc gcaggcaatg ggctctaccg
     1561 cacttcgacc accgacgtgc tgatcaatgc cattcgcctc atccggccgg gaacgctgcc
     1621 gcccaatacg aattggggca tcactccgct cctcgtgtct ccatcctatt cgggtggcca
     1681 cagctttatc ggctatctct ccgccgaccc cagccggacg gacctcttgt ccgatatcga
     1741 gtcttcgctg accgcactgg ccgcggttaa tccctgttag ctgggcacat ccattacatt
     1801 caggtgtgag tggcgaaggt gccgccaagt gtccatgcca aacctattgc ggcggctata
     1861 acaaaggaca taagctcttg tgtcttaaga aaggagggcc ccatgagaaa gacactgagc
     1921 gttctaggct cttgtttcgt ctttgcgatc gcggctgcga tttcacaagc ccaagaggta
     1981 cctcctgcct cgccggctgc accggaagcc gcggcgaaag caactcccgc ggacggccat
     2041 gtcatccacg tcactgcacc gcacttgacc gatggaaaga tacgggggcc atttcatcat
     2101 tattgcaaga tcgtctctcc tgagccgttt atcgagtgcc ttctctacga gacggaagac
     2161 aacaacgcga aactcgtcgg tgttgagtac attgtcgcca agactgtgac gcgtgacgag
     2221 aaggttcttc ccaagaaggt gtggaaaaag gtttggcatg atcattccgt ggaaggtgtt
     2281 atagggaaca tcaagatact ggatatgtct gccgacaagg ccaaggagtt cgcagacatg
     2341 gttgcccaga ccgacggggt catcttttca ttatggcctc acggtgccaa attgccgacc
     2401 ggtaaagtca gcattggtac gatggtcggg cacgctgtgc atcaatgaca tcggatcaac
     2461 cagataagac cagatccgcg ccacgcgcgg caaattgatt cctcgcaccg ggtattggag
     2521 cttcaagcaa caactgaagc gcctcgctct acgggtttgc gcttgcggta agctcacggt
     2581 ataaccacgg cgggagtgga tgaggatcag tccaaaggcc ggatcgatct gcgcgtgctc
     2641 ttcgttccaa ctcttctaat atcacatctc tcggagcata cttccgattc caccagcacc
     2701 agccattttt gacgagaact tggttcactt ttcttccatc cggtaggaac acgtctgcga
     2761 tcgtgtgacc atgcctgtcc ttgccgtgcg tctggagcat gacatctttg ccaaaaacga
     2821 ggtcagatgt tgcctgcttc gctcgttggc cgaacgtctg gcctttctcc gggcaatcga
     2881 tgccattcag gcggatgcgc tcaggctttt ggtactgcaa gacttcgatc gtgtcgccgt
     2941 cgatgacaga gacgacgagg gtggagaact cggtcgcccc gccgtcaact acaaacagga
     3001 aggctgcgca tgacaagaag aacgcgcgaa gtccagttgt ggtttttggc aacttctcaa
     3061 aaagatcgat gcgcctctgg acgaacgcgc caccgagact catcatccgc ttcagagttt
     3121 ttgaggaaga aatcattacg ctccagattg tgagaagcct ctgataaata aggtttgcaa
     3181 tgcctgggcc aatcgatcgt tggttcttgg agacgatttg atccattgaa tatgcaaggc
     3241 tggcgtaggg ggttcatcac gccatgcgga attcaaccag ggacatttgt aagatgggca
     3301 ctgactcgga aacgattctg gctgaatgtg aaatgatcca gaagcagtcg gcgaccacag
     3361 cctaataaaa gaaggccttg aacgtggaga ccgcgattgt ggaagccgtc aagtgactca
     3421 agacttcagc cacgcaaggt gcagcaccct ggcgactcaa tgcctcgaac aggttgactg
     3481 cggtttgtca gctcgctgtc cctatacaat caggatgaag gacaaggggc taatacaaac
     3541 aactaccaga gggctaccgt tctctttgaa ggctaacagg atccgccacg cgcagagtaa
     3601 tggtttctga ctcactttca ctgttagatg tttgaccctg gtcgaggaat tgccgacgcg
     3661 gtactgttct taatctgcag aactaaaatg aacggtgtca gctgtagcta gttgtcccgc
     3721 ggcagtaaat gttaggacga gccggtctga ggttcctcta gtagacactc gccaagagca
     3781 ggctttgcct ccatgatcta aattgacaca gctttcagga gcgcccaagt tctgcttagc
     3841 ctcgtcgagt gaaaaatgac cgattctctt ttgccaatca gttatggcca tctgagcgca
     3901 agctgccatg acaaggcagc atacagatat agtgatcatt cggaacatgg atcagagtct
     3961 aatgcaaaat cgtacagtat ccaaccctat caggaggcgc ggctggatgt tgaagaccgg
     4021 cgatggagta aggaacggaa aagcagtcac gtgtcgtgaa ataattattg ataccgtctc
     4081 tcaagcacat agcaaagtag cctgtcacct tttcatatcc cctggttcat ctatagcggt
     4141 ttccctgata gccagccagt aatttcaagg gtaacctcca agatagctct cgctaagaac
     4201 gctagcacac gggcacttcg tgccataaac actgaaagga ttgaaggagg aaaccaatga
     4261 acatacttct tggtctgttt ggaaatcgcg cgaaggcacg acgggcaaag ggcgcactca
     4321 ttcgcgccca tatcgccagt ccgaacattg ttctttatga tcgttcggca gcggactctt
     4381 caagggaagc agaacaaagc ctgtgggagt ctcttaagga ttcttcggcg ttcgcagagg
     4441 accgcagctt ctatgaagaa ggcatgcgcc gcggaggcac tgtgatcagc gttcgcgtta
     4501 atgacgaaca gatggatcaa gctgcggaga tcctgtcgag agagggagct attgaggcgg
     4561 cgataccaga ggcccaagaa aaacttcaag gcggtcagcg gcaagtcgtg aggggaacag
     4621 tccgcgtgta taagcgagcg gcatgacgcg agtcccgaca ccacacgcgg gtatcgcaat
     4681 gcaaatggcc aaaaaagcgc ggattctggc cacgaatccg tggacgcgaa agaggataca
     4741 agcggcggca gttatttctc acgataggcg ccttagagga tcgcgacgaa cgatcctggc
     4801 gccggacggt cgaatagatt ccaggcgctc ccgccccgga ggaagagctc tatccagcgc
     4861 accgtgcttc catcacggag cgtccaaccg ctgctgcccg gtgcgaatgc cagttcgccg
     4921 tgaggcacgg cgaacgcccc atcgctgacc gtggcctcat gctgacgctc gttgattcgc
     4981 acggtgatcc gcttgcccgg tcgaacgcga ttcgggtcgt aggcaatggt cgtcttgagg
     5041 ttgccgtaac cgtctacata tgccaccgct gagaggggag gcgaaggaat gctttccgga
     5101 tcgaggtcgt gtcccaaagc ggcgggcagg cccagcgcaa tggctgccgc cgcttgagga
     5161 aatacatctc gtgatcggaa ctggcttcct tcggccgaag acgctgccca gcggagtttc
     5221 tcggccacat ctttgataaa ggaaagggcg taacctgcgt tgaccccgat cacgcggacg
     5281 ccggttggca ggcgtgcgta agcgagacgc tcgccggcgt tctgcgcacg ggcagcggga
     5341 tcgtcttccc gcggggccac gttgtgatag atgagcgtac cgggaggcga aggatgcagg
     5401 ccgatctgtg cgatgcaaaa gccggccgcg atggtggaga aggccggcac aggtgtcaat
     5461 actggttccg tatcgggcaa atggaccttg atccgttgca cgacttcggc gaaggcgagg
     5521 tctcccattc cgtaatcggc gatgatgtgc accaacacag cggccttctc tcaggtaatg
     5581 tccgtcgttg cggggatcaa cttatgtcgc ggttgtctca atctgatgcc acaggaccga
     5641 gcatgagaaa gtaactagga cattctacgt tttcacccgg gcggcagtct atcgcggtgg
     5701 caggtgcgat gaggggtggt gagggggcga ggggggacaa cccggccgcc tcgcgcgcga
     5761 aatggttggc gacacccaca gctgtaggcg gtagcttata gacaacgtct cggtcgagag
     5821 atcataatgc gtgtaattat ccttcagtca tttccatcta atcatttcgg cctcatttcc
     5881 agctcagtaa gtcactaatc gtgtgaagac aggttcggct cgttccgaac ggtcgttgac
     5941 atgagcatac ggcaggagat gcattttgaa tcaggagttc tcaaagtaga tgcaaggggg
     6001 gagttctcgc tggaggaggc caagcgcgca tttttagaga tgctggaggc tgtggcccaa
     6061 tatcaggccg cgaaagtact cttcgatggt cggaacctga agggagagcc tgcgagcatc
     6121 gagcgcttct actatggcta tttcgcggcc acagaaacga ttcacctcat cggcatgaaa
     6181 cacatgcgcc aggctccgca gtttgcgtat gtgatgaatg agcctcttcg tgacccgcaa
     6241 aggtatggag aaaccgtcgc tgtgaatcga gggatgaatg tcaagacctt tgaaactcca
     6301 gaagaggctt tcgagtggct cgaacgccct ccagccaaca acccggatgg ttgacacgcc
     6361 tgcccttttt cttaccgcct gattcgctgt acctgccgtt agacggcttt gctagtgacg
     6421 ctagccacgg tggctcacca ggttcacacc gcaggcgaag gtcagacgaa attccagctc
     6481 atatcgacgc agttgcggcg ttgggttttt acttgccctc gagcgaggcg gttttcactt
     6541 ttcctgtgac cataaagggc atcaacggaa gggtcttcgc ctctttatgc accttcagga
     6601 tcgcaatggc gccacgcaag gcatctgtga gggaatgcgt tacgatggga atgacgcctt
     6661 ctcctttatc caacacgata tccatcgtgg ccgctcccgc aggaggcacc ataacggttt
     6721 gcacgcctcg gagacgattg gcgggattgc cgctctccca tacatcctcc caaagctcag
     6781 cgatgggatg gactgctgaa aagttgttag ggcctgcatt cacgaagtag atacgcaccc
     6841 gctcccctgg cttggcttcc aagaagtctc ctccactctt gccgtggacg ggatcgtatc
     6901 gaaagacccc tccgttgaag accacatgct catatttcct atcgaacata gcctgcacat
     6961 catccggatc tttaaacagc tcgctttgga ccaagacata ttcacgatcc gctttcggca
     7021 tggctttccg gttctttgga tccacgatga tggcgcctat cattccacgc gccacgtgct
     7081 gaatcatggg agaggcgccg caatgataca cgaagatacc cggctctttg gcttcccacg
     7141 tgtactgcag tgactctccc ggttggagtt ctcgataatg agtgagaaag tcagtttggg
     7201 cggcgtggaa atccatcgaa tggggcctgc tgcttgtgtg tgtattggtg aagctgaaat
     7261 caactcgatc accctgttgg acgcgaatga ctggaccggg atactgatta ttgaaagtcc
     7321 acgcctgata ggtcgtgccc tcgccgtcga ttaccacctc agcttcaacc gctgtcatat
     7381 cgaccttgac ggtctttgcg tccgaaatcg ccgccatggc aagcatcgcc accgaccctg
     7441 cgagaatcaa cccttgccgt gctgttatca ttatgacccc tccttatgac ttctccttgt
     7501 ttatgagcct tacagacgat tcttggatac tgcatataca taaaagaaga tactggtgat
     7561 gttcttagtt tcggcctctg ctataccagg gacatgacga tcaaggcatt gcaccacgtc
     7621 agcgtcgtcg tcgacgacct tgcagccgct atcgcttttt tcaccgcgct cggaatgacg
     7681 atcgaaggcg agaggccggt cgagggcccg tgggtggacc gcgtcaatgg gctcgaaggt
     7741 gtccgagtcg acatcgtgtt aatgcggacc ccggatgggc acggacggct tgaactgacg
     7801 aggtttcgca atccaaaact ggtcgagatg gaaccggcga tcgcaccg
//
LOCUS       DASSCQ010000052        10792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1893739, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000052 DASSCQ010000000
VERSION     DASSCQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893739"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(580..1728)
                     /locus_tag="VFH05_03775"
     CDS             complement(580..1728)
                     /locus_tag="VFH05_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6677359.1"
                     /translation="MKVYEHQRPVGLALVLGNGLVTSSGEAWKRHRRIMQPVFHRSRV
                     ASMADRMAQVGEQRLEGWADYVGRSVDIAAEMMQLALEVISQTMFTTSVTDHMDQISH
                     ALRVSLKYAFDSFHNPLRPPLWIPTARNREFRSVMQFMDRLVYGLLAERRRSGANHDD
                     LLDLLIRARDEETGAGLSDQELRDEALTIFAAGHETTANALAWTWYLLATHPEATARF
                     HEEIDRVLQGRMPKADDLQHLPYTRAVFDESLRLYPPVPAIQRKATTGATVGKLSLPA
                     GAVVLIGTYNLHRHPAFWRDPDCFIPERWLDGERPASRCAYLPFGAGPRACLGMYFAT
                     VEGPLLLALIGRRYDLKLAQERVKPEVMITLRPKGGIRMTLHGRNNLS"
     gene            complement(1736..1951)
                     /locus_tag="VFH05_03780"
     CDS             complement(1736..1951)
                     /locus_tag="VFH05_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677360.1"
                     /translation="MQEPVQLFTLNDVPGAFPLLGHLRLFKKDPLNTMSTWWREHGDA
                     LRFRLGPKMFYLFSHPDLAEEILVQAI"
     gene            complement(2384..2887)
                     /locus_tag="VFH05_03785"
     CDS             complement(2384..2887)
                     /locus_tag="VFH05_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6677361.1"
                     /translation="MRRWVRIKQNDHDKWSRFRTQFQQLLDSAIAADNTAKGNVQLWN
                     PNLNALEIVAQRGFDNIFLRKFEVVHVNEPSACGRAFRLGRRVMIHDVTLDRFYEPYL
                     SIAQASGYRAVQSTPILRADGAVIGVLSTHFPDRHEWEEAAQRALDQYASQIAALVTE
                     LVESAAI"
     gene            complement(3105..3494)
                     /locus_tag="VFH05_03790"
     CDS             complement(3105..3494)
                     /locus_tag="VFH05_03790"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HET6677362.1"
                     /translation="MLTGMYSSLALAEPMMIVPVGPRIDTTSDLSFRHEQAGFIITLV
                     PWAISTIGKAVSIDGGTGPAFFSNYKFAGRDFGGPVQIVSTVGAGFSVIPGCFVEYRL
                     QHFSDAGAYGPYKVGVDMHLLEINFRF"
     gene            4733..5104
                     /locus_tag="VFH05_03795"
     CDS             4733..5104
                     /locus_tag="VFH05_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6677363.1"
                     /translation="MTKDRLIAIVDDDDALRGSLDNLIRSVGYRARGFSSAEAFLKSN
                     HLHDTVCLILDVRMAGMNGLHLQREIVAAKWPIPIIFMTSYADEAVRVEALAAGAMDF
                     LYKPFMEDSLLKALDKAIQHS"
     gene            5273..5482
                     /locus_tag="VFH05_03800"
     CDS             5273..5482
                     /locus_tag="VFH05_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677364.1"
                     /translation="MDTIRRDVLNLITANEKIQSLLLRGEEFTKDEAAVVRMCATELL
                     SRVPDRDAVDEPDGGVNAQSLAIRH"
     gene            5660..8869
                     /locus_tag="VFH05_03805"
     CDS             5660..8869
                     /locus_tag="VFH05_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6677365.1"
                     /translation="MRSLFLRYGLAIGLSSVVLLVTVLAPHLAREGVYILALAAIVFT
                     ARYGGRGPGWLATLVSALTIDYFLIHPIGTFWITSWEAVFVLTVFVVVAIKLVEFSET
                     RGRDHSALQASEERWKAVFENTPTMYFMVDQSGIILSVNPFGAEQLGYRVEELVGTPV
                     LDVFHEADREAVQRHVSTCLTHVGQPMMWEFRKVRKDGRIIWVRETAKTVRFGTSERV
                     VLVACEDITERKRAEEAAQAAKVRFEGILEIAQDAIISVDSNQQIILFNQGAEKVFGY
                     TQAEIIGRPLDLLLPQRVEDVHRKHIDDFARSPDVPRTMGQPREVSGRRKDGGVFPAE
                     ASISKLDLGSQPVFTVILRDITERKRTEAMLAGEKRVLEMVAKGDSLSQILDSLCRLV
                     EEQAPGILASILLVEHNLLRHGGAPSLPKAYIDAIEGVAIGPCVGSCGTAAYRGEQVI
                     VSDIATDPLWADYRAAALSHSLQACWSSPIFSSEGKVNATFAMYYRESRSPGPHEQEM
                     IGQITHLAGVAIQRARAAEKLRESEARYRILVEHAPEAIVVLDVDAGRFVDANRNAVR
                     LFGLSREALMRVGPIDVSPSTQADGCSSSESILEKIRDAVDGGEPVFQWTHLNAAGEQ
                     LSCEVRLVRMPAAGHVLVRGSVTDITERKQAEQRLLAQHTVTQILAAAASLQEVTPRI
                     LRAICENLIWDMGALWTVDNEAGVLRCVEVWHEKSIEIPEFEAETRATTFRPGIGLPG
                     AVWRSRKPIYLSDLSTKERAMFPRAPIAAREGLHAAFAFPILLSGEVLGVIDFFSREI
                     REPDQDLLDMMITVGSQIGQFIERKRAEDTVRKIQLELAHVVRVSTLGELTATIAHEI
                     NQPLGAIVNNAGASLRWLAAQNVDEVRQSIERVIKDGHRASEIISRIRALVKKSAPQK
                     DPLRLNDTISEIMSLVGPELQTHEVAMHTHLSPDVPTVLGDRIQVQQVLLNLMMNAIE
                     AMTITKEGPRELWVGAEKHDAQYVEVRVQDTGPGIAPQQQDRLFEAFYSTKPGGLGMG
                     LAICRSIVEAHGGRLWATANEDRGASFHFTLPIREERSA"
     gene            8866..9492
                     /locus_tag="VFH05_03810"
     CDS             8866..9492
                     /locus_tag="VFH05_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677366.1"
                     /translation="MTETDGVVFVVDDDASMRESLKNLTRSVGLRVEAFASAQEFLRV
                     KRSDEPGCLVLDVRMPGLSGLELQKRMAETGTDLPIIFLTGHGDIPMSVRAMKAGAME
                     FLAKPVREQDLLDAIQQALERDRAMRHQRTNTEHLRERFDLLTAKEREVMGKVAAGLL
                     NKQIAGELGMSESTVKIHRHQVMEKMRAGSLAELVRMADRLGLPAPKP"
     gene            9729..10082
                     /locus_tag="VFH05_03815"
     CDS             9729..10082
                     /locus_tag="VFH05_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6677367.1"
                     /translation="MATILVLDDDANVRRLLRAVLESSGYEVLDAAEGRAGLTLYRQR
                     AADLVITDIRMPTLDGLGLMIELTREFLNVKVIAMSGTRELLGRAKLLGARHTFHKPF
                     DMKELLSAVKYELAH"
     gene            complement(10457..>10792)
                     /locus_tag="VFH05_03820"
     CDS             complement(10457..>10792)
                     /locus_tag="VFH05_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677368.1"
                     /translation="LRAACGLNAPLREQPGMTTQEEEESSIRQEYSNKRYLYDGFCRE
                     ALRQVDEVISREGIALAFLIEHRVKSLESILEKRTRMPHKHRVPCRHARCRWAANYHL
                     VPPGSRSSN"
BASE COUNT         2421 a         2931 c         3116 g         2324 t
ORIGIN      
        1 aggttgcggg tcaaaggcga acccggatgc gagatacgca ccggatgcat ttcggacgat
       61 gaagacctgt ttgccatcga tggacagctc tggcttttca ggcgttccgc taacgacgat
      121 atgccgtccc ttgtatgtct cccggtgttc agccattggc ctatgatccc aagggttcag
      181 acgcctacca taacttttgt taagattgac gccttctccc agtttcggaa attagattca
      241 tgcaactatc tctcggctga tcgctatatc cgtcgcacca ccggtttgca tgggatccag
      301 tgcgcgtgaa acggcatcgc tcatagtgca cggcgaccaa gatagcgcct ttttcgaggg
      361 tggccttcat agcgcgtcgc ctcatactag aaggatcaca cgctgacacc tactaggatt
      421 gctgccagtt ctcaatagga cacgctcggt gggaatttac gcatataggc gactgagaag
      481 aactggatgg aggcatgtat gagagtcttg atcaccttag ccgtaggagg cctaatgctt
      541 tcttgcctga ctggtgtgga agctgccgga aaagagtatt taacttaggt tgttgcgccc
      601 gtggagggtc atgcgtatac cgcctttcgg acgcaaggtg atcatgactt caggcttgac
      661 ccgctcctgc gccagtttca ggtcgtatcg gcgcccaata agcgcgagga gcaatggccc
      721 ttccactgtc gcaaagtaca tccccaagca ggcccgcggt ccagcgccaa aagggagata
      781 ggcgcaccgc gaggctggcc gttcgccgtc caaccatcgc tcaggaataa agcagtcagg
      841 gtcgcgccag aacgcggggt gacggtgcag gttgtacgtg ccgatgagaa cgactgcgcc
      901 agccggcagc gagagtttcc caacggtagc gcctgtcgtc gccttccgct ggatggccgg
      961 aactggtgga tacaggcgga gcgattcatc gaacacggca cgtgtgtatg gcaggtgctg
     1021 aagatcgtct gccttgggca ttcgcccctg cagcacccgg tcgatttctt cgtgaaagcg
     1081 tgccgtggct tcggggtggg tcgcgagaag gtaccacgtc caggcaagcg cgttcgcagt
     1141 cgtttcgtgt ccggcagcga agatcgtcag cgcttcgtcc cgcagttctt gatcgctgag
     1201 cccggcgccg gtctcctcat cgcgagccct gatcagcagg tcgaggagat cgtcatggtt
     1261 cgccccactt cgacgccgct cggcgagtag tccatagacg agcctatcca tgaactgcat
     1321 cacggaacga aattcacggt tacgtgcggt cggtatccac aggggcggcc gcagtggatt
     1381 gtgaaaagaa tcgaacgcgt atttaaggct cacgcgcagg gcatggctga tctgatccat
     1441 atggtccgtt acgctcgtcg tgaacatcgt ttgcgagatc acttccagcg ccagttgcat
     1501 catttccgca gcgatgtcga cggaccgtcc cacgtaatcc gcccagcctt cgagccgttg
     1561 ctcccccacc tgcgccatcc gatcagccat tgaagccacg cgggatcggt gaaagaccgg
     1621 ctgcatgatg cgccggtgcc gcttccacgc ttcaccggaa ctcgtcacta atccgttgcc
     1681 caagaccagc gcgaggccaa ccggccgctg atgttcgtag actttcacga accgatcaga
     1741 ttgcctggac gagaatctct tcggccagat cggggtgact gaacagatag aacatctttg
     1801 ggccgagcct aaagcgcagc gcatcgccgt gctcacgcca ccacgtcgac atcgtgttca
     1861 acgggtcctt cttgaagaga cgcagatggc ccaacaaggg gaaggcgccg ggcacatcgt
     1921 tgagggtgaa gagctgaacc ggctcctgca tggtaggacg aaccgtatta cattaaagta
     1981 ggatcggtct accggccgct ccacttagat tggaggtagc gagagccggt ggtttgggac
     2041 gttctatgtt gatagcgagt cagcaagccc acgcagcagt attgccccca tcaccatgcc
     2101 atgcactgcc gtccatgcac gcgagtgtag cggcaaaacg ctttgccacg cttcacgcac
     2161 cagtggaaac attggttaaa ggatacgccc ctttgcccga tcgattacaa ccggcgtatt
     2221 aatgcgataa attccatcag taaccttatg cgcattcgag tagtttctga gtacgctgga
     2281 ccaaagggcc aagtgctttc gtaaaggcct gaacggctcg tggggtatac gttttaaagg
     2341 attcgtgaac gctgcctgac cggtttaccg aagtagcaca gaatcagatt gcagcacttt
     2401 cgaccaactc tgtcaccaat gctgctattt gggaggcgta ttgatctagt gcacgttgtg
     2461 cagcctcttc ccactcgtgc ctgtcgggga agtgagttga aaggaccccg atcacagcac
     2521 cgtcggctcg tagtatgggg gtggattgaa cagcacgata gccgctggcc tgggcaattg
     2581 agaggtatgg ctcgtaaaag cggtcgagag tcacatcatg aatcatcact cttctgccga
     2641 gtcgaaaagc tcgaccgcat gcagaaggct cattgacatg cacaacctcg aatttccgca
     2701 aaaaaatgtt gtcgaaccca cgttgtgcca cgatttctaa tgcattgagg ttcgggttcc
     2761 agagttggac gttccccttg gcggtattgt ctgccgcaat tgcggaatcc aaaagctgtt
     2821 gaaattgagt acgaaaacga gaccatttgt cgtggtcgtt ttgttttatg cggacccatc
     2881 tgcgcatcgt gggcatcata tcacagctac tgcgctcagg acacattccc ccaaagtacc
     2941 ttcacagagt tcccacgacc ttcctccaaa ctacgacaaa ggacatgagt ggctggtgcc
     3001 cctaatgcga cccgtcacaa aggagagtgt ctactgcagc cctctttctt caaaagcctt
     3061 ttgcgggatg ccgcatgagc cgtcgggctc cctgcagacg cctctcagaa tcggaagttg
     3121 atttcgagca gatgcatgtc cacaccgact ttgtagggac cgtacgcgcc agcatccgag
     3181 aaatgctgaa ggcggtactc tacaaaacat ccgggaatga cactaaaacc ggcgccgact
     3241 gtgctgacga tttggaccgg gcctccaaag tctcggccag cgaatttgta attgctgaaa
     3301 aaggcaggtc ccgtcccgcc gtcaattgag acggcttttc caatggtact gatggcccac
     3361 gggactaaag tgataatgaa accggcttgc tcatggcgga acgacaaatc actggtggtg
     3421 tcaatgcggg gtccaaccgg gacgatcatc atgggctccg ccaacgccag agatgagtac
     3481 atgcctgtga gcatgatagt caccatcatg gaagttaggt acagagtgcc gagattctct
     3541 agcaggcaat ccctcaagag agggggcaac caggtttgga cggatgaggg gcacgcaacc
     3601 ataaaccacc caaattgtgc ttctaatttg ggcgcaatcg gctgtctgtc gccggctgca
     3661 accgggacgc ataaaaacac tcttgctcaa tttcatgcct ttctatgcta gtgaggcccc
     3721 cagaatgtcc taatggtttt atctacgtac actctatgtt tttattgatc cttcctagcc
     3781 gcacctttgc ggcttcagcc aggagggaca aatatgtggg gataagggag caccttcagt
     3841 ttggaatctt tacgcaaaag ggagatcgaa atgacgcagg gtcaggttat ggcgcaaaag
     3901 gtcgtaatcg ggaagtcgct ccctgatctg aagccgatcg agtactgtgg taacgacgag
     3961 aagccggtcc agcgtcacat gatcgtggaa tttgtccagg caaaacgctc agtagtgcga
     4021 cagtgacttc ctgtgaatcg acgcctgggt cggatatgcc cggatcgtcc ggttgagttc
     4081 tcgatgtggg ctagattctt catcttgatc caatagggcg cctgtgtagt gagcacaaaa
     4141 aaagagggga tgaagagatg cccagccgat accttcgagc tggtcatctc acagcttagt
     4201 tcagctgcct aaagcagccc ctccacaggg tcctagacgg ttccgtgata gcgagagatg
     4261 taagggtggt ttctagtccg tggaaagtgg tgggccgtgt aggggtcgaa cctacggccc
     4321 gctgattaag agggagaaag agccattttt acaaaagctc gttattgcaa tggtttcccc
     4381 aaatcggttg taatttcatt agcttgaagc gttccattag ttctatcctt tcccgtcctt
     4441 tcctttccct tcccgcctag tctggtccca aaaatggtca cagtgcgata ggcgatttac
     4501 gaatttcaga aagtccagcc tccgacaacc taaacccttc ctaccagagc cgtagcggtc
     4561 gccaaacctc agtctccgat accggacaac cagctacgca taccggtcat gggcggggct
     4621 cgcaagctcg tcgacctgac taaaggtcgt acctgcctgt cctaacgtcg tagagacgtt
     4681 tgtctctcaa aaccggtaca tctctctacc gatgcaggga aggatttgaa tcatgacgaa
     4741 agaccgcttg atcgcgattg tggatgacga cgatgcacta cgcgggtcgc tcgacaatct
     4801 catccgatcc gtcggatatc gggcaagggg cttttcgtcg gctgaggcgt tcctgaagtc
     4861 caatcacctg catgacacgg tctgcctcat tctcgacgta cgaatggctg gtatgaacgg
     4921 actccatttg caacgggaga tcgtggcggc caaatggccg atcccgatca tcttcatgac
     4981 atcctacgcc gacgaggccg tgcgcgtgga agcgctggcg gccggtgcca tggacttcct
     5041 atacaaaccg tttatggaag atagtctgct caaggcgttg gacaaagcca ttcagcattc
     5101 atgagccaac aatgaggcca agcgaagttg agactcgagc ggagcaccct ccaattcgag
     5161 attcagaaac acggcatcgc tcggcagtgc tagcagagga ccgtaacatc aaagccggct
     5221 tgctgattat cagtggcgag agcttcgtat gacggcagcc catggaggtt acatggacac
     5281 aattcgacga gatgtactca acttgatcac cgccaatgaa aagattcaaa gcttgctgct
     5341 tcgtggagag gagttcacaa aggacgaagc cgctgtcgtc cgcatgtgcg cgacagaact
     5401 actttcgcgc gtgccggacc gtgatgcagt agatgagcct gacggcggag tcaatgccca
     5461 atcgctcgct attcgacact gatccatgat gatcccggcg gtgagccgct cgatgtgttc
     5521 attctgcgcc cgtacccagg gacggtgctg tttcagcagc cggtgtatcc ctgattatac
     5581 gattgtctaa gcgttcggag tttcgctatc ctagtccttc cgcagtgcca tgcccgctga
     5641 ggagcgatct gccgtgcaga tgcggtctct tttccttcgt tatggtctcg caataggctt
     5701 gtcctctgtt gtcttacttg tcacggttct cgcccctcac ttggctaggg aaggcgtata
     5761 catccttgcg ctcgctgcca ttgtcttcac ggcgaggtat ggcggacgag gaccgggttg
     5821 gctggccacg ctggtctcag ccctgaccat cgactacttc ttgattcatc cgatcggaac
     5881 tttctggatt acatcatggg aagcggtgtt cgtattgact gtctttgtgg tcgttgcgat
     5941 caagctggtg gaattcagcg agactcgtgg acgtgaccat agtgcgctac aggcgagcga
     6001 ggagcggtgg aaggccgtat tcgagaacac tccgaccatg tacttcatgg tcgaccagtc
     6061 tggcatcatc ctgtcggtga atccgttcgg cgccgaacaa ctcggctacc gagtcgagga
     6121 actggtgggc acccccgtgc tggatgtgtt tcatgaagcc gaccgcgagg ctgtacagcg
     6181 acatgtgagc acctgcctga cgcacgtcgg tcagccgatg atgtgggaat tccggaaagt
     6241 ccgcaaagat ggccggatca tctgggtgcg cgagacggca aaaacggtcc gcttcggtac
     6301 gagtgaacgt gtggtcttgg tggcgtgtga ggacattacg gagcggaagc gggcagaaga
     6361 agcggcgcag gccgcaaagg tccggtttga agggattctg gaaatcgccc aggacgcgat
     6421 tatatccgtt gattccaatc agcaaatcat actgtttaac caaggagccg agaaggtttt
     6481 tggatatacg caagccgaga tcatcggccg cccactcgat ctgctcctgc cccagcgagt
     6541 tgaagacgtc catcggaaac atatcgacga ctttgctcgg tcgccggacg tgccgcgaac
     6601 gatgggacag cctcgcgagg tgtccggccg tcgaaaagat ggaggcgtgt ttccggccga
     6661 ggcctccatt tccaaactcg accttggcag ccagcccgtt ttcaccgtta ttctgcgcga
     6721 catcactgag cgaaagcgaa cggaggccat gctggccgga gagaagcgcg tcctggaaat
     6781 ggtggccaag ggcgactcgc tgagtcagat cctggacagc ctctgtcgcc ttgtagagga
     6841 gcaagcaccg ggtatcctcg catccatcct attggtcgaa cacaatctgc tccgacatgg
     6901 tggggctccc agcctgccca aggcttacat cgacgccatc gagggagtgg cgattgggcc
     6961 ctgcgtcggt tcatgcggca cggctgcgta ccgaggcgag caggtgatcg tcagcgacat
     7021 cgccaccgat ccattgtggg ccgactaccg tgcggcggct ctttcccact cgctccaagc
     7081 ctgttggtcc tcgcccatct tctcctcgga gggcaaggtt aacgcgacgt tcgccatgta
     7141 ctaccgggag tctcgtagtc ccggcccgca tgagcaagaa atgatcggac agatcactca
     7201 cctggccggc gtcgcaattc aacgcgcgcg agcggcggaa aagctgcgtg aaagtgaggc
     7261 acgatatcga atccttgtcg agcacgcacc cgaagcaatc gtcgtgctgg acgtagacgc
     7321 gggccgattt gtagacgcca accggaacgc cgttcggctt ttcgggcttt ccagagaagc
     7381 tctgatgcga gtggggccca ttgacgtaag tccctccacg caggccgatg gttgttcatc
     7441 cagtgagtca atactggaga agatacggga cgccgtggac gggggggaac cggtctttca
     7501 atggacccat ctcaacgcag cgggagaaca gctctcctgt gaggtacgcc tggtgcggat
     7561 gcctgccgcg ggccacgttc tcgttcgcgg aagcgtcact gatatcaccg aacgcaaaca
     7621 ggcggagcaa cgcctcctgg cacaacatac cgtcacgcag atactggcgg cagcggcgag
     7681 cctccaagaa gtcacaccac gaatactccg ggctatctgt gaaaacctga tatgggacat
     7741 gggagcgctt tggacggtgg acaatgaggc gggcgtactc cgttgcgtcg aggtgtggca
     7801 tgaaaaatcc attgagattc cggaatttga agccgaaacg cgcgcgacca cgttcaggcc
     7861 cggaatcggg ttgccgggtg cagtgtggcg gagccggaag ccaatctacc tttccgacct
     7921 cagtacgaag gagagggcga tgttcccgcg tgcgccgatc gcggcgcgtg aaggactgca
     7981 tgcggcgttc gcctttccca ttttgctcag cggcgaagta ttgggcgtca tcgacttctt
     8041 cagccgcgag atcagagagc ccgaccagga tctcctcgac atgatgataa cggtcgggag
     8101 tcagattgga cagttcatcg agcggaagcg ggccgaagac actgtccgaa agatccaact
     8161 ggagctcgcg cacgtcgtcc gggtctcgac gctgggcgag ttgacggcga ccatcgccca
     8221 cgaaatcaac cagccgctcg gcgccattgt caacaacgcg ggcgcgagcc ttcgctggct
     8281 ggctgcccag aacgtcgacg aggtgaggca gtcgatcgag cgagtaatca aggacggcca
     8341 tcgtgcgagc gagatcatca gccggattcg cgccctcgtt aaaaagtcgg caccacagaa
     8401 ggacccgcta cgtcttaatg acaccattag tgaaatcatg tcgctggtag gtccggagtt
     8461 gcaaacccat gaagtcgcga tgcatacgca tctgtcaccc gatgtgccga ccgtcttggg
     8521 cgatcgcatc caggtgcaac aggtgctgct caatctgatg atgaatgcca ttgaagcaat
     8581 gaccatcacc aaggaaggtc cgcgcgagct gtgggtgggg gccgagaagc atgacgcgca
     8641 atacgtggag gtgagggtac aggacaccgg gcccgggatc gcgccgcagc agcaggaccg
     8701 cctgttcgaa gcgttctatt ccacgaagcc tggcggactc ggcatgggac tcgcgatttg
     8761 tcgttcgatt gtcgaagcgc acggaggacg gctgtgggct accgcgaacg aggaccgggg
     8821 ggcctccttt cactttacat tgccgatcag ggaggagagg tcggcatgac ggaaacggac
     8881 ggcgtggtat tcgtcgtcga cgacgatgcg tcgatgcggg agtcgctcaa gaacctcacg
     8941 cggtctgtcg ggctccgggt cgaggccttt gcctcggccc aagagttcct gcgagtgaaa
     9001 cggtcggatg aaccaggctg cctggtgctc gacgtgcgaa tgccgggatt gagcggactg
     9061 gaactgcaga aacgaatggc tgagacgggc acagatcttc cgattatctt tcttaccggt
     9121 catggtgata ttccgatgag cgtgcgcgcg atgaaggccg gagccatgga gttcttggcc
     9181 aagccggtcc gtgagcaaga cctgctagac gccatccagc aggcattgga acgcgaccgt
     9241 gccatgcgtc accagcggac aaataccgaa cacctacggg agcggttcga tttgcttacc
     9301 gcgaaagagc gcgaggtgat gggaaaggtc gctgccgggc tgctcaacaa acaaattgcg
     9361 ggagagttgg gcatgagtga gagcacagtc aaaattcacc gccaccaggt gatggagaag
     9421 atgcgagccg gttcccttgc cgagttagtc agaatggctg atcggttagg acttcctgcc
     9481 ccaaagccgt agtatggtgc tgccgtcctg ccagtcccgt gtcatcgcat ttccgtacaa
     9541 tgcctcatga tgccgaaaga attcttcgcg accagccatt tggcacgctc cgacggtcgg
     9601 gctcgccaac tcgtcgacct gaccaaaggt cgtacctgcc tgtcctaacg tcgtagagac
     9661 gtctctctct cgaacccggt agcatctcgc tacggatgaa agatttgaat catgacaaaa
     9721 gacccccgat ggccacaatt ttagttctgg acgatgatgc taacgttcgg aggctcttac
     9781 gcgcagtgct ggaatcatct ggctatgaag tcctagacgc agccgagggg cgggcggggc
     9841 tgacgctgta tcggcaacgt gcggctgatc tggtgatcac cgatattcgc atgcccactc
     9901 tcgatgggct aggcctgatg atcgagttga cgcgtgagtt cctcaatgtg aaggtgattg
     9961 cgatgtcagg tacaagggag ttgctagggc gagcgaaact cttgggcgcc cgccacacgt
    10021 ttcacaagcc atttgatatg aaagaattgt tgagtgcagt gaaatatgag ttggcccact
    10081 aagcgacgat tcggtctcgt tttcgagaag tctcaacagc aaatgcgtta agcgatcacc
    10141 ggaggataat tgtgaagcag gccgaagcac ttcgcacctt ctgtagtggg tgcgagcggt
    10201 tgctgttcta cataaggacg aatggatctc tcgccgagga cgaagcgcgg atggtcgaac
    10261 actactgcaa ggaaatcctg gccaaggtag gcgcctatgg caaaggccag tcaaattcga
    10321 gttgaacgcg agcttcaaaa ccagacgtgt ctggacagag tctttctcaa ctagaactgc
    10381 acattctaaa gcgatctgcc tctagccgag actgaacatc ctccatgtgc atgacatgga
    10441 agttattctt gatcagtcag ttactcgatc gagatcccgg cggaacaaga tgataattcg
    10501 cagcccagcg acatcgcgca tgtcggcaag gcacccgatg tttgtgcggc atcctcgttc
    10561 tcttttcgag aatcgattct aatgacttga cccggtgttc gatgagaaag gcaagggcga
    10621 ttccttcacg cgagataacc tcatctactt gccgaagagc ctcgcggcag aatccgtcgt
    10681 agaggtaacg cttattggaa tactcctgtc gaatcgagga ttcttcctct tcctgagtag
    10741 tcatgcccgg ctgctccctt aaaggcgcat tcaatccaca tgcggctcgt aa
//
LOCUS       DASSCQ010000053         7896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1569076, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000053 DASSCQ010000000
VERSION     DASSCQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569076"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFH05_03825"
     CDS             <1..283
                     /locus_tag="VFH05_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HET6677369.1"
                     /translation="AIFEKRSDAEAKGNHVGDGQGEGDAIYVITHDRCTECVGHFDEP
                     QCAAVCPVDNCCISDPAYPETTEVLLEKAKKLNPDKGIDPAKVWSGVRN"
     gene            412..714
                     /locus_tag="VFH05_03830"
     CDS             412..714
                     /locus_tag="VFH05_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677370.1"
                     /translation="MPEKIPKGISSATVIKALAKVFAFQAIEISLLRSHAGISDAHWK
                     ALKTEAFKKTYAKQRSFYEDRLTGAFALERISQSERETRLLELWLKQSEDSSKDEP"
     gene            954..1376
                     /locus_tag="VFH05_03835"
     CDS             954..1376
                     /locus_tag="VFH05_03835"
                     /inference="COORDINATES: protein motif:HMM:NF039665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6130 family protein"
                     /protein_id="HET6677371.1"
                     /translation="MKHIPILLRAVAMGSGVVIAQTQTSTPTQEPPAKLIVDAPLPDL
                     LAKGIVWITWRVENVNIGPESGKDALNASPRVGHLHIHVDDLPWLWAHMSTAPIDVAL
                     LPPGPHKIRIELVNAIHQNVPEQSKTVTFTIPDGARPH"
     gene            1599..2003
                     /locus_tag="VFH05_03840"
     CDS             1599..2003
                     /locus_tag="VFH05_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6677372.1"
                     /translation="MGLGRILVVDDEAHIRTTVRMALTKAGYDVVEAEDGAKGIQSIK
                     SGDNPLLVDMILCDMQMPNIDGIGAISFFLQQFPSVPVVVMTGHPDINTATKLMKQGV
                     KDYLVKPIEKEKLLAVVENAVKHNGRKDRFHV"
     gene            1960..3372
                     /locus_tag="VFH05_03845"
     CDS             1960..3372
                     /locus_tag="VFH05_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET6677373.1"
                     /translation="MRSSTTGAKTDSTYNSPALDQGGRDGKVRPKEKLPSEHGALHNQ
                     RRAPRRTVDLRVNNGSRELETALLISQALSRHCTLDDLIVQTLDTALEAIKAEGGSIL
                     IADASAQQLVLYRAVGEKPVLPGSAVPWNHGIAGAVFQSAQHAIIVDADKDPSLPERI
                     DKSTGLVIKNLVIVPLKRWQGEPIGVIEILNKRDRPIDEDDVAVLTMISSLATPAIEE
                     ARLFEAKKLAEVAQMAGEMSHDIKNLLMPVLCGAELLKDELEELFDKWSIRKTPESEK
                     SRTMCHEVLAMVEEDVRRISDRVRELADCVKGMTSQPRFASCRVVGVVEAVFKTLDLM
                     AKKKGVALRSTGLDDLPSLEADEQRLYNAFYNLVNNAISEVPSGGSVTVLGAVAKTAN
                     VLDICVTDTGRGMPPEIRDSLFTAHAISRKSGGTGLGTKIVKDVVDAHKGHINVESTV
                     GAGTTFRLILPLRQVKTGHS"
     gene            3638..4186
                     /locus_tag="VFH05_03850"
     CDS             3638..4186
                     /locus_tag="VFH05_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677374.1"
                     /translation="MPRRSAVLTSFPLFPVATHKFIVLSVVSFTAYTFYWFYQNWKRI
                     KTASQENLSPFWRTAFAPLWGFSLFRHIRTLASAQGIAADWSPNLLGTVFVVLNVLWN
                     LPAPWWWISLAVFVPAIPVQQTAQRVNELYATAVGEGRNDSYSPGNVAMIVIGGLILA
                     LAIMGSFMPDSVPLPIFTVVMR"
     gene            complement(4403..4729)
                     /locus_tag="VFH05_03855"
     CDS             complement(4403..4729)
                     /locus_tag="VFH05_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6677375.1"
                     /translation="MGSKLYVGGLPYSATEQQLSDLFAAHGTVASARIIADKFTGQSR
                     GFGFVEMSSDSEAKAAITALNGSDMGGRSLTVNEAKPMEQRSGGGGGGFGGGGGRGPG
                     GKRDRW"
     gene            5061..5618
                     /locus_tag="VFH05_03860"
     CDS             5061..5618
                     /locus_tag="VFH05_03860"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HET6677376.1"
                     /translation="MKTFQSLWIASIFLVAAACGGVGQRNPLTTVPFVDLSRYSGTWY
                     EVARLPMWFQRHCVDSKAIYTSRPDGTVGVHNECKTDSGGLDQVDGVATVVDQKTNAR
                     LSVVFDNFFARLFGSSRQGNYWILDLDPDYRAAMVGTPDRRYLWILSRTPRIDEPTYE
                     LLVNKALELGFPVGDLIRTRRSSPP"
     gene            5982..6152
                     /locus_tag="VFH05_03865"
     CDS             5982..6152
                     /locus_tag="VFH05_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677377.1"
                     /translation="MRLSDDVHKFTSACEHLLADMAILRPLTPDEVLLVKHYCNEVLA
                     KIDSPPTNPRRP"
     gene            complement(6435..6629)
                     /locus_tag="VFH05_03870"
     CDS             complement(6435..6629)
                     /locus_tag="VFH05_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677378.1"
                     /translation="MTKYLLSPIVCVLVSLLSGCTTERHYPAPPQTVIYEEKSSADTR
                     SDAREAAREGAREGARDTYR"
     gene            complement(7294..7509)
                     /locus_tag="VFH05_03875"
     CDS             complement(7294..7509)
                     /locus_tag="VFH05_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677379.1"
                     /translation="MSRILWTLPVVLMAGLVVAGCADDPNPGRQRPAYSYENVDMGSP
                     QPGQYRFDERPPASNTDPYSRSQAGRY"
BASE COUNT         1817 a         2214 c         2091 g         1774 t
ORIGIN      
        1 ggccatcttc gaaaagcgca gtgacgccga agcgaaagga aaccatgtgg gcgacggcca
       61 gggcgaggga gatgccattt acgtcattac gcatgaccgc tgcaccgagt gtgtcggcca
      121 ttttgacgaa cctcaatgcg ccgcggtctg cccggtcgac aattgctgca tttctgatcc
      181 tgcctatccc gaaacgacgg aggtcttgct ggagaaggcg aagaagctca atcctgacaa
      241 gggtatcgat ccggcaaagg tgtggagcgg cgtgcggaac tgatcgtgtt tgatccgagt
      301 tccgcaccac gtgcagaatg ctgagaactc tgtccgtaca gcgctggctt gaagttcgac
      361 gagaacaccg tatggctatg ttttgccttg gcataaccga aacgatcagt catgcctgag
      421 aaaattccca aaggaatttc tagtgcaact gtcattaagg ctttagcgaa agtctttgct
      481 ttccaagcca tcgagatcag cctgctgcgg tcacatgctg gaatatccga tgcccattgg
      541 aaagccctca agaccgaagc ctttaagaaa acctacgcga agcaacgttc tttttatgaa
      601 gatcgcttga cgggtgcgtt cgctttggag cgcatttcgc agtctgaacg cgaaacgaga
      661 cttcttgaac tctggctgaa acagagtgag gattcttcta aagacgaacc gtaaaccagt
      721 gctctcggca tcggcgcatg cccttcctgc tgagtgatgc caggcgctga aatgttcggc
      781 attgccaata gcttttctag ggctggcagt gcatgatgca gtacagcttc agcgcttccc
      841 cggatacctg gaccgggaat cagccagcac gaagacttcc cttcctattg actctctcag
      901 atcggaagcc caggctttcc ctctagatga acccgcagga aaggagaccc ttcatgaaac
      961 acattcctat tttgctgcgt gccgtggcga tgggatccgg cgttgtcatc gcccagaccc
     1021 aaacatctac accgactcag gagccaccag ccaaactgat cgtggatgct ccacttcctg
     1081 acctgctggc caaaggcatc gtctggatca cgtggcgcgt ggagaacgtg aacatcggtc
     1141 cggagtccgg caaggatgcc ctcaacgcat ctccacgggt tggacatctc cacatacacg
     1201 tagacgacct gccttggttg tgggcgcaca tgagcactgc cccgatcgac gtggccctgt
     1261 tgccgcccgg cccacacaag atccggatcg aattggtgaa tgccatccat cagaatgtgc
     1321 ccgagcagtc caagacagtg acgttcacca ttcctgatgg cgcccgtcct cactgacgcc
     1381 agcaactttc tcggcatctg gcgaggtatg caccttacgc aggcaagccg aatgcgattc
     1441 atgccgccat tgctccctta ggtcagcatc atccatccga gtaggtctcg cccagtttcc
     1501 aatcatattt cgactattcc ttgccaaaca caaagccttg ttgcaggatg tcggagatta
     1561 cttttgggtc acttaccgca acagcaaagg agggcactat gggactgggc agaatccttg
     1621 tggtcgacga tgaggcgcat atcagaacga ctgtcagaat ggcattgaca aaagccggat
     1681 atgacgtcgt cgaggcggag gacggtgcaa aaggaattca gtcgatcaag tcgggtgaca
     1741 atccgctcct ggtggatatg attctctgtg acatgcaaat gccgaacata gacggaatcg
     1801 gggcaatctc attttttctg cagcaatttc cctctgtccc tgtcgtcgtc atgacaggcc
     1861 atccggatat caataccgcc acgaagctta tgaagcaggg cgttaaggat tatcttgtga
     1921 aaccgattga gaaggaaaag ctgctcgctg tcgtcgagaa tgcggtcaag cacaacgggc
     1981 gcaaagacag attccacgta taattctccc gcacttgacc aaggcggaag ggatggcaag
     2041 gtgcgcccta aagagaaact gccttcggag cacggcgctc ttcacaatca gcgccgcgcc
     2101 ccgcgacgaa cagtcgatct tcgtgttaac aacggcagta gagaattaga aaccgccctc
     2161 cttatctctc aagcactttc acgtcactgc acgctcgatg atttgatcgt gcagacgttg
     2221 gacaccgctt tggaggcgat caaggcagaa ggtgggtcta ttctgatcgc cgatgcctca
     2281 gctcaacagc tcgttcttta tcgcgccgtt ggtgagaaac cagtcctccc tggatcagca
     2341 gtcccgtgga atcacgggat tgcgggtgct gtttttcaat ccgcccaaca cgccatcatc
     2401 gtggatgccg acaaagatcc ttccctccct gaaagaatcg acaagtccac agggctggtg
     2461 atcaaaaatt tggttatcgt gccgctgaag cggtggcagg gcgaaccgat cggggtgatt
     2521 gaaatcctca ataagcgcga tcgccccatc gatgaagatg atgtcgccgt tctgaccatg
     2581 atttcttcgc ttgcgactcc tgccatcgag gaagcaagac tattcgaagc caaaaaactt
     2641 gctgaggtcg ctcagatggc cggggagatg agccatgaca tcaagaactt gctcatgccg
     2701 gttctatgcg gagctgagtt gctcaaagat gaactcgagg agttgttcga caagtggtcc
     2761 attcggaaaa cccctgagtc ggaaaaaagc cgtacaatgt gccacgaagt gctcgcgatg
     2821 gtggaagagg atgtccgaag aatctcggat cgggttcgag agttagcgga ctgcgtgaaa
     2881 ggcatgactt cccagccaag atttgcatcg tgccgggttg tcggcgtggt cgaggcggtt
     2941 ttcaaaacgc tggatttaat ggcgaagaaa aaaggagttg cactcagaag cactggcctg
     3001 gatgatctgc cctccttaga agcagacgag caacggttgt ataacgcctt ctacaacctg
     3061 gtcaacaatg ccatatcaga ggtcccatcc ggcggctccg tgacggtgct cggagcggta
     3121 gccaagacgg ccaacgtgct cgatatttgt gtgactgata cgggccgcgg tatgcctccg
     3181 gaaatccgtg atagcctctt caccgctcat gctatcagta gaaaaagcgg cggaacagga
     3241 ctcgggacca agatcgttaa agatgtggtc gatgcgcata aaggccacat caacgtcgag
     3301 agtactgtag gtgcagggac cactttccgt ctgattttgc cgcttagaca ggtgaagact
     3361 ggacactcgt agccagctcg tcctcccacc ctttcgtttt ctcctatagt ggcgagacaa
     3421 ggaaatggat aggctacatt tcaagtctgt cgcctgaaac ccttacgcgg cggtgccggg
     3481 agagatagaa acagtgtcgc ttaccacctg tccagattgc ggtcactcga tctcagacct
     3541 agcccctgcc tgcattcatt gtggacgtcc acgtgacctg accgtctcat ccgagaccgc
     3601 ctcgcttcaa gcgggcagca catcagggtt gccgtctatg ccacgacggt ctgccgtgtt
     3661 aaccagtttt cccctctttc ccgtcgcgac gcataagttc attgtcctct cggtggtcag
     3721 ttttaccgcc tacacttttt actggttcta tcagaactgg aagcgcatca agaccgcctc
     3781 gcaagaaaac ctgagccctt tttggcgtac ggcctttgcg ccgttgtggg gattttctct
     3841 gttccgccac attcggaccc tggcgtccgc gcaggggatc gcagccgatt ggagccccaa
     3901 cctgctcggc acggtcttcg tggtcctcaa cgtcttatgg aatcttccag ccccatggtg
     3961 gtggatcagc cttgctgtgt tcgttccggc gatccctgtt caacaaacgg ctcaacgcgt
     4021 aaatgaactc tacgccacgg ccgtcggcga aggtcggaat gatagctata gtcctgggaa
     4081 tgtcgccatg atcgtcatcg gaggattgat ccttgcgctg gccatcatgg gatcgttcat
     4141 gccggactca gtgcccctgc ccatattcac cgtcgtgatg cgttagcgtg ccggcgacca
     4201 gaacacagtg ccaggaatca cttctcggcg tgtagcaggt cgttcgttcc tcgcctcccc
     4261 gctccgtcac cagtcgtcgc acctgaatcc gcatagacac cgcccatcgc atgacacatc
     4321 gcggatggga gcctctgcat caaaaaaaaa gggtgccatg ctttcatggc accctttcgc
     4381 ctgttcgatc agcgtgaacc gcttaccagc gatcgcgctt tccaccgggg ccacggccac
     4441 cgccgccacc gaatcccccg ccgccgccac cggaacgctg ctccatcggt ttcgcttcat
     4501 tgaccgttaa cgagcggcca cccatgtccg agccattgag cgccgtgatc gcggccttcg
     4561 cctcagaatc cgaggacatc tccacgaagc caaatccgcg ggactgtccc gtgaacttat
     4621 ctgcgatgat tctcgcagaa gccaccgtac catgcgccgc aaacaaatcg cttaactgct
     4681 gctcggtcgc cgaatagggc aacccaccga cgtaaagctt cgaacccatg tggggttcct
     4741 ccttataaat gtgacattgt cttgaactcg aggaacgggg gaaggagcgg gccgaagacg
     4801 cgggcaatgc ggcgacttag gtctgacttc cgacaagatt ctcggacaag gcaacatcga
     4861 tgttgcggac cttcctctta aaccagctcg tcgcgaggcc cgtacgacga cgagccaacc
     4921 gtaacacagg gggtgatggt ttacaacctt acacatgatg acaagcaaaa gggggcattt
     4981 acttccattt aaggaaccat tgtgcgggtt gacgccgtac ctcctatgtc atagcctgtg
     5041 aaaaaccccc gagatccttc atgaagactt tccaatcact ctggatagcg agcatctttc
     5101 ttgtggccgc tgcttgtggc ggtgtggggc aacgaaaccc gctcacaacg gttcccttcg
     5161 tcgatctctc ccgttattcc ggcacctggt atgaggttgc gcggttgccg atgtggtttc
     5221 agcggcactg tgtggattcc aaagcgattt acacgagccg tcctgatggc acggtcggcg
     5281 tgcacaacga gtgtaagacc gatagcggag gacttgacca agtggacggc gttgcgactg
     5341 tagtcgatca gaagacgaat gccaggctca gcgtggtctt cgacaatttc ttcgcacggc
     5401 tcttcggttc ctcacgtcaa ggaaattact ggattctcga tcttgatccg gactatcgcg
     5461 ctgcgatggt gggcactccc gatcgccgct atctctggat tctgtctcgt acgccccgca
     5521 ttgacgagcc tacatacgag cttctggtca acaaggctct agaacttggc tttcccgtag
     5581 gagacctcat ccgtacgagg cgctcttcgc ctccgtaaac cgtttcgcac atgcttcgac
     5641 ccaagatcca cgctccaatg ttccacctcg agcgccttgg ttgtctgggc tcatcggggt
     5701 cctcccctcg gagcggtgtc ggttttggtc attgcggaag gctagtgaag aaaaggggaa
     5761 acacgcctgg ctgtcgcggt ccccttctca aaagcgtact gacttgtagg ctgccgctga
     5821 tagacaaata tgtccatagg cgatattggt taggaaacac gcctaaactg aagacgcatt
     5881 ttgaccggca gtacgtaacc aagccgcgaa tcagcggacg gaaacgcgtg cctcgctgcc
     5941 aggatcctac cactgcatcg ctcaaatgaa aggatgcatt catgcggctc tcggatgacg
     6001 tgcataaatt taccagcgca tgcgagcacc ttctcgcaga catggcgata cttcgcccgc
     6061 ttactccgga tgaagtgttg ctcgtcaagc actactgcaa cgaagtgtta gccaaaatcg
     6121 actcccctcc aacgaatcct cgacggccct aggaacagtg gccagaacca ttccgcgcgc
     6181 gtgggccggc tgctctcggt ccaactcctt cgcctcgcac caaactccac caacattctg
     6241 cttttctaga acatgcacgc cgatattctg cttctccgtt gaaagggcgt tgcgtgatta
     6301 tgggggcgtg atgcgattaa accaggaaga gatgaaacga aagggagcgg cgcgtggcgg
     6361 gccggcgtca ttccggctcg ccgccagacg tcgctccatt attcgtagcg agaaccactc
     6421 gtgcgtagcg caaactaacg gtatgtatcg cgggcgcctt cccgtgcccc ttcacgagcg
     6481 gcctctcgcg catccgagcg cgtatcggca gatgacttct cctcataaat tacggtctga
     6541 ggtggtgcgg gataatgccg ttcggtcgta cagccggaaa gcaggctcac cagcacacat
     6601 actataggcg acagcaaata tttagtcatg gggtcctcct cggtttgacg atggtgcaag
     6661 cgtcaggccc gtgtctgaag gccgtgagga gattgaaaaa cgtattcaga aatcaaggac
     6721 atgcaaccga atcacgtccc gtccctactt tcgattccac acactttgtc attggtgggg
     6781 acaagatgtc ctgcctctca tctggacaag aggcgatggg ttctagatat caccccgatg
     6841 tttcatctta gggggttcgc attgatgaag tcatcctgcg tgatacaaag gaaacggtgc
     6901 cggaccgctt cgcggctggg tgacgggaac ctggatcatc tggtagcttg gtcgttcaga
     6961 gcggtcttgg aatgaatgaa cgtcttcgcc ttgaaactct gatatcaacg gacgggctcg
     7021 agcaggccag gcaatgggct aggaagacgg cagttgttta tggcgattcc ctcaatgagc
     7081 cgtcccacta tgcctcacaa ccagactgga agtccctctt taagaagagc atgcaagagc
     7141 taacacattt tgcggaaact ggcgagatac catgaagtca ttgacatgaa aacattctgc
     7201 gtttcacggc atatggcgag ccccgttggc tcatcgcact tcagacggca atcgacaggg
     7261 gggaacccgt caaatttccc ttcaagtgtt cccctaatag cgcccagcct ggctgcggct
     7321 ataaggatca gtgtttgaag ctggcggacg ttcgtcgaaa cggtattgac caggttgtgg
     7381 tgatcccatg tcgacgttct cgtaactata cgcgggtcgt tggcgacctg gattagggtc
     7441 atcggcacaa ccagcgacca cgaggccggc cataaggaca acgggcaggg tccataaaat
     7501 acgactcatg ctgaacctcc ttggtcaccc tcagttattc cgccttacac tctagaaagg
     7561 ttgttcttcc cttccttttc atacaatcag tctatgtcct taccagacaa tgcataccta
     7621 gcaagccccc ttgaaacacg aaaaggggga cattcacgtc acgaaatata cccagtttaa
     7681 taccatttcg gcgtcgcggg caccccataa ttgcatccat agctagtgat gactgcaacc
     7741 ggccattgac tctctccttc aatgtcctca aaatttccct atcgttcgcg cattcattaa
     7801 caaggttttg acagcggtcg gatacgagca ggtcctggct gaacaaggag gtgcgatcat
     7861 ggggaaaatc gttgagtgcg ccaaggttga tccatc
//
LOCUS       DASSCQ010000054         9929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1397520, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000054 DASSCQ010000000
VERSION     DASSCQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397520"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFH05_03880"
     CDS             <1..375
                     /locus_tag="VFH05_03880"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HET6677380.1"
                     /translation="FEPIKESSDELGVKQGKSGGPTKAMASQTAPKSPAGDRRDLTKT
                     QARLEKQVARAEAEIAEHEAKIKERDQMLADPALYQEFSKWNGLHQEQDTWKQELERL
                     TARWESLSSDLADVKQKLAAIA"
     gene            complement(401..790)
                     /locus_tag="VFH05_03885"
     CDS             complement(401..790)
                     /locus_tag="VFH05_03885"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6677381.1"
                     /translation="MKRAHSKSHTSSAAANERRKLVRATLVGSALVSPKAGAKAITAV
                     LDNVNRVGAGLHAKDKLVNGEKVIVSLAFLDSDRVEQQERLDGTVAWVKTWEKGYLIG
                     VVWDEIVTKEKNKWLYYYLEETIKSSA"
     gene            complement(842..2134)
                     /locus_tag="VFH05_03890"
     CDS             complement(842..2134)
                     /locus_tag="VFH05_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET6677382.1"
                     /translation="MKRPYEPAADLFDVPKREGAANAPLAERMRPQSFDDLAGQEEIV
                     GKGKSLRSAIEADRLSSVIIWGPPGCGKTTLAHLISRSTKAHFVAFSAVTSGIPELRE
                     ILKGAEHRLRTSGQKTILFVDEIHRFNKAQQDAFLPHVEKGTIILVGATTENPSFEVV
                     APLLSRSLVAVLRPLNDEALERILDRALTDADRGLGGRRIQIDQDARRRLIGFANGDG
                     RSLLMALEFVAEQAVPQPDGSRRLDDATVEAALLKKTLRYDKSGEEHYNLISAYIKSL
                     RDSDPDGAVYWLARMLEAGEDPKFIARRLVIFASEDIGNADPLGIVVAAAVAQAVQFV
                     GLPEAQINLAHGTTFLATRPKDNASYTALLEALSDARAEGNLGVPLHLRNAVTSMMRD
                     LGYGKGYRYVHDDPQAQREQEHLPEALKGRKYYKPKGK"
     gene            2247..3050
                     /locus_tag="VFH05_03895"
     CDS             2247..3050
                     /locus_tag="VFH05_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6677383.1"
                     /translation="MKTQVQGVTIAYDDQGAGLPIVFLHAFPLNRSMWSEQVAALSRG
                     FRAIAIDLRGHGESDAPFWRYSLEQYALDVKEVLAGLGIRKALFVGLSMGGYLEFTLY
                     RLYPDLMLGVVLADTRAEADKPEQIQWRYDLAQRTAAIGPAAVIAEMLPKLLAPKTYE
                     RNPDLVARVKSMQAAAPVPGIIGDLIAIAERPDSTDLLSSMTIPALVIVGQEDVLTTP
                     ADAERIANGIHGAQLIVIPNAGHLSNLEQPERFTSAIEAFATGLQKTNA"
     gene            complement(3047..3688)
                     /gene="folE"
                     /locus_tag="VFH05_03900"
     CDS             complement(3047..3688)
                     /gene="folE"
                     /locus_tag="VFH05_03900"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET6677384.1"
                     /translation="MPRRRRRASITHVEDLPAVSAPQDLTTRTGLVSQLLRALGESPR
                     RNGLLKTPERVAKALLFMTRGYHEDIERLLNGALFPIEYDEMVIVKDIDFFSMCEHHM
                     LPFFGKCHVGYLPNKKVVGLSKIPRVVDAFARRLQVQERLTTQIAETLKRKLDAHGVA
                     VVMEARHLCMMMRGVEKQNTLAVTSSMLGAFRTQEQTRNEFLKLIRRNAVGEP"
     gene            complement(3708..4121)
                     /locus_tag="VFH05_03905"
     CDS             complement(3708..4121)
                     /locus_tag="VFH05_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET6677385.1"
                     /translation="MPHVSLTRRYRFCAAHRLHTDFLTPQQNWAAFGKCNNPNGHGHN
                     YVVLVTVKSETGQEVYQFEHLDQMVEKTIVSRFDHQDLNQDPAFINTTTTGENLVKLI
                     WDLLIAKMPEGSLEKVGLIETRDNYFEYAAPAAVS"
     gene            complement(4250..4570)
                     /gene="erpA"
                     /locus_tag="VFH05_03910"
     CDS             complement(4250..4570)
                     /gene="erpA"
                     /locus_tag="VFH05_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET6677386.1"
                     /translation="MVTITPVAEQKIHELMKEEKDVVGLRIYVRGGGCHGYQYGMAFE
                     SKMAEDDTVIEKGDVKVIMDSQSAPLLQGAEVDYVDSVQGSGFSIKNPQAKTTCGCGS
                     SFSA"
     gene            complement(4627..4971)
                     /locus_tag="VFH05_03915"
     CDS             complement(4627..4971)
                     /locus_tag="VFH05_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6677387.1"
                     /translation="MPLVTFLHPKGKSGTVPVNTTVLDAAKELGFPLNHDCGGNASCT
                     TCRVEVQSGSDHLSEIDFEEQDLLDREALSEPWHRLGCQAKILGDVVVKVPETKWAAP
                     TTQSEAQGVDIR"
     gene            complement(4982..6280)
                     /gene="nuoF"
                     /locus_tag="VFH05_03920"
     CDS             complement(4982..6280)
                     /gene="nuoF"
                     /locus_tag="VFH05_03920"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET6677388.1"
                     /translation="MTLTEARLIEKTDGPPWDIDAYVKVGGYEAWKKCVKGLTPDQVV
                     QELKTSGLRGRGGAGFPTGVKWDKVLNHRVKERYFVCNAGEHEPGTFKDRYLLKTIPH
                     QLIEGCLIASRTAQAKASFIYVNHEYHEERENLKKALAQAKERGLVGKNILGSGIDID
                     LQVFEGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDYGLYGKPTLVNNVETLCNI
                     PRILHKGAAWFTQVGTEKCPGTMMFSLSGAVNRPGVYEMPMGVTVRELIEQCGGGVPG
                     GRTIKAVFPGGPSFSMLTADQLDLPMDFDSLKKAGTGLGSAGVIVVDDATCMVRQTLK
                     FSNFFKGESCGQCPPCRMGTINLATLMTKIEAGEGTQKDLDSLLQLCGFVKGTGYCTL
                     VTGASVLVQSSVKLFRHEYEEHIRLQRCPYPSVPVEAVAH"
     gene            complement(6498..7085)
                     /locus_tag="VFH05_03925"
     CDS             complement(6498..7085)
                     /locus_tag="VFH05_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET6677389.1"
                     /translation="MKFLEDPMQTMGAGFALALVLIVLYLGMTGIGAGEVDWTGIVIR
                     WIHFLAGITWIGLLYFFNLINAAFLKSLEGPTKNIVIPKLMPSALNWFRHGATVTVLA
                     GIILYIKLYMNGGTGAYALGIGGLLGIIMMVNVHAIIWPNQKKIIAAVTAAAQGTPAP
                     ADMAQWGRTALLASRVNFLLSIPMLFFMGAGSHLK"
     gene            complement(7178..8119)
                     /gene="mdh"
                     /locus_tag="VFH05_03930"
     CDS             complement(7178..8119)
                     /gene="mdh"
                     /locus_tag="VFH05_03930"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET6677390.1"
                     /translation="MGRPKITVVGAGNVGGTTAQRLAEKDAYDVVLVDIVEGVPQGKA
                     LDISQAGPVCGYSTKVVGSNGYEESAGSSIAVVTSGMPRKPGMSRDELLATNAKIVSS
                     VVTELVSRSPDIILILVTNPLDAMVHVARNVSGLPKSRIIGMAGVLDSARMRTFIAQE
                     LNVPAPDVEAMVLGGHGDTMVPLPRYTTVKGKPISDLMPKHRLEAIVQRTRDGGAEIV
                     GLLKTGSAFYAPSASAVDMVEAIHKDQKRVMPCAVFCDGEYGLRNVVVGVPVKLGKGG
                     AEQIMVYDLTPAEQKALEASANAVRELCATVDRVMTN"
     gene            complement(8195..8638)
                     /locus_tag="VFH05_03935"
     CDS             complement(8195..8638)
                     /locus_tag="VFH05_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677391.1"
                     /translation="MLDVDDKNHRNGSMTDQPEHRSVPPWLYKLFTGHQYPYVRRLAK
                     FAQRFTPGEERLEPTKAMIEAKFWEVYPQCSVKILQEVKTGMIVVFHDLGEYPPGGYQ
                     ELIDNPEDFLAKTYGKKKIKVNFYHGENFVCTINFKVAGWTEHEH"
     gene            complement(8667..>9929)
                     /locus_tag="VFH05_03940"
     CDS             complement(8667..>9929)
                     /locus_tag="VFH05_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6677392.1"
                     /translation="EELLASRADLLESEQPADDSTETASGQSAPEQADNEYRPVSNAF
                     YRGEMHPEMIGGDVEKSDSEVQPPGGGSIERIPAERGRFGEEHSLGGDLLGGGHSLPA
                     VVEELLTQDVQPQLSHESAVPGGQTIRYPEWDYMLQDYRVNWCRVLERPADSGSDETV
                     SATLTAHRSDIRSLRRFFESLRPPAFRRLAGQADGEDLDLDAVVRRAAEQAAGFEGSD
                     RVYVRREKKDRDVAVALLVDVSGSTGRQLEGGRRVIDVERDSLVLLCEALEAVGDQYA
                     LYAYSGQGRGSVDVLMIKGFDERLGPATAQRLGGLSPRQQNRDGAAIRHAVAALMRRE
                     AKTRLLLLLSDGRPLDGDYKEEYALEDTKAALREANRHGIHPFCVTIDREADGYMRRM
                     YGDVHYTVIDRVESLPARLPRIYRRFAT"
BASE COUNT         2062 a         3014 c         2644 g         2209 t
ORIGIN      
        1 ttcgaaccaa tcaaggaatc cagcgacgag ttgggtgtga agcagggaaa gtcgggcggc
       61 ccgacaaaag ccatggcctc gcaaacagca ccgaagtctc cggccggaga ccgccgtgac
      121 ctgaccaaga cgcaggcaag gcttgaaaaa caagtggcca gggcggaagc cgaaatcgcc
      181 gagcacgagg caaaaatcaa ggaacgcgat cagatgctcg cggacccggc gttgtatcag
      241 gaattttcaa agtggaacgg actgcatcaa gaacaggaca cctggaagca ggagctcgaa
      301 cggctcacgg ccagatggga atcgctgtcc tcggacttgg ctgatgtgaa acagaaactg
      361 gcggcgatcg cttagaaagg caggcggctc acgtgatctg ctatgcgctg gatttaatcg
      421 tctcctcgag atagtagtac aaccatttat ttttttcttt cgtcacgatt tcatcccata
      481 ccacaccgat aagatagccc ttttcccacg tcttcaccca ggccacggtc ccatccagcc
      541 tttcctgttg ctcaacccga tccgagtcga gaaatgcgag tgagacgatg actttttcgc
      601 catttaccag cttgtctttg gcatgcagcc cggcacccac ccgattcacg ttatccagca
      661 ccgccgtgat agccttcgca ccggccttgg gcgacaccaa tgcggaaccg actaacgtgg
      721 cccgcacgag cttccgccgc tcgttggcgg ccgctgacga cgtatgactt ttcgagtggg
      781 cacgcttcat ggggctaagt ataaccgaat agaaatcttt gtccaacgcc ggcagtccag
      841 tctatttccc ctttggcttg tagtacttgc gtcctttcag cgcctcgggc aaatgctcct
      901 gttcccgctg tgcctgcgga tcgtcatgga cataccggta gcctttgccg taacccaaat
      961 cgcgcatcat ggacgtgacg gcatttcgga gatgcagggg aacgcccaga tttccctcgg
     1021 ccctcgcatc tgacagcgcc tcgaggagtg cggtgtacga cgcattgtcc tttggtcgag
     1081 tcgccagaaa ggttgtgcca tgcgccaggt tgatctgcgc ctcgggcaac ccgacgaact
     1141 gaacagcctg tgccacagcc gccgcgacga caatgcccag cggatcggca ttgccaatgt
     1201 cttccgacgc aaaaatgacc agacgtctcg cgataaactt cggatcctcg cctgcttcca
     1261 acattctcgc cagccaatag acggccccgt cgggatcgga atcgcgcaga ctcttgatat
     1321 aggccgagat gaggttgtag tgctcttctc cggatttatc atagcgaagc gtttttttaa
     1381 gcagggccgc ttctaccgtg gcatcgtcaa gccgtctgct gccgtccggt tggggaacgg
     1441 cttgctctgc cacaaattcc aaggccatga gtaatgaacg tccatcgccg ttcgcaaagc
     1501 cgatgaggcg tcgacgtgcg tcttggtcta tctgaattcg ccggccgccc aatccgcggt
     1561 cagcgtccgt gagggcccgg tcgagtatgc gctccaaggc ttcgtcgttc aaaggccgca
     1621 gcactgccac caacgagcgc gatagcaagg gtgccacgac ttcgaaggag ggattttctg
     1681 tggtggcgcc cacgagaata atcgtgcctt tttctacatg agggaggaag gcatcctgct
     1741 gagctttgtt gaaacgatgg atctcgtcga cgaacagaat cgttttctgt ccgctggtcc
     1801 tgagccgatg ttcggctcct ttcaggattt cccgcaattc aggaatcccg cttgtaaccg
     1861 cggagaaggc gacaaaatgg gcctttgttg atcgtgagat cagatgggcc aacgtggttt
     1921 tgccgcagcc tggcggtccc cagatgatca cagaggacaa acgatccgct tcaatcgcgc
     1981 tgcggagcga tttccccttt ccaacgatct cctcctgccc cgcgagatca tcaaacgatt
     2041 ggggacgcat ccgttcagcc agaggtgcgt tcgccgcgcc ttcgcgcttc ggaacgtcga
     2101 acaaatcagc ggccggttca tatggacgtt tcatgccaac cctcgacgac caaaattcta
     2161 tacgcccctt cggattcagc gcaaattcaa cttcggcagt cttgacccgt ccgggacggc
     2221 ggtgttacga cacgccaagg acttcgatga agactcaggt gcagggtgta acgatcgcct
     2281 acgacgatca gggagcaggc ctcccgatcg tctttctcca tgcctttcca ctcaaccgca
     2341 gcatgtggtc tgagcaagtg gctgcgctca gccgggggtt tcgagcaatc gcgatcgact
     2401 tacgcgggca cggtgaatca gacgctccgt tctggcggta cagtctcgaa caatatgcct
     2461 tggatgtgaa ggaggtactt gcgggcctgg gtatccgtaa ggccctcttc gtggggctct
     2521 cgatgggtgg atatctcgaa ttcaccctct accgtctgta tcccgacctc atgctcggag
     2581 tcgttctggc ggacacccgc gccgaggccg acaaaccgga acagattcaa tggcgctacg
     2641 accttgctca acggaccgcc gccatagggc ctgcggcagt cattgccgaa atgctgccaa
     2701 agctactggc cccgaagacc tacgagcgga accctgatct agtggctcgc gtgaagtcta
     2761 tgcaggcagc ggcgcccgtc cccggaatta tcggcgacct gatcgcaata gcggagcggc
     2821 ctgattccac agatctctta tcgtccatga cgattcccgc gctggtgatt gtcggacaag
     2881 aggatgtctt gaccacgccg gctgacgccg aacggatcgc taacggcatc catggcgccc
     2941 aactcatcgt aattccgaat gctggacacc tcagtaacct cgagcaaccc gagcgtttca
     3001 cctcggccat cgaagcgttc gcgacaggcc tgcagaagac caacgcttaa ggttcaccga
     3061 cggcgtttct tctaatcagt ttcagaaatt cgtttcgggt ttgttcctgc gtccgaaacg
     3121 cccccagcat ggagctggtg accgcgagag tattctgttt ttccacccct ctcatcatca
     3181 tgcacaggtg gcgggcttcc atgacaacgg cgacaccgtg ggcatccaac tttcgcttga
     3241 gcgtctcggc gatctgcgtc gtgagccgct cttgtacctg cagtcgtcga gcgaaggcat
     3301 cgacgacacg cggaatcttg ctgaggccga cgaccttttt gttcggcaaa tacccgacgt
     3361 gacattttcc gaagaatggc agcatgtggt gctcgcacat actgaaaaaa tcgatatctt
     3421 tgacgatgac catctcgtcg tactcgatgg gaaacaaggc gccattgagc agtcgttcga
     3481 tgtcctcgtg ataccctcgg gtcataaaca gcagcgcctt ggcgacccgc tccggcgtct
     3541 tgaggagccc gttccggcgt ggagactcgc ccagcgcacg caagagttgg ctgaccagac
     3601 cagtcctcgt cgtaagatct tgtggagcgg aaacggcggg aagatcttcc acatgtgtga
     3661 ttgaggcgcg gcggcggcgt cttggcatag gctctcttat gctccggtta actgacagcg
     3721 gcaggcgccg catattcgaa ataattatct cgggtctcga tcaggcccac tttttcaagg
     3781 gagccttcgg gcatcttcgc gatcaggagg tcccaaatca atttcaccag attttccccc
     3841 gtcgtggtcg tgttgataaa ggcagggtct tgattcaagt cctgatgatc aaagcgcgag
     3901 acaatcgttt tctcaaccat ttgatcgagg tgctcgaatt gatagacctc ttgtcccgtc
     3961 tcactcttga ccgtgaccaa cacgacgtag ttgtggccgt gcccgttggg attgttgcac
     4021 ttcccgaacg ccgcccagtt ctgttgagga gtcaaaaaat cagtgtgtag tctatgggcg
     4081 gcacagaagc gatagcgtct ggtgaggctc acgtgtggca tgtggttcgt cctggatcgg
     4141 gtgccggctg gggctcaatc gtcgaaaaca caaaagccag ggctactaat cgagcaaccc
     4201 tggctttgtc atctttcact tgtccccggc gagccgaggt cacgccgctt tatgcgctga
     4261 atgagctacc gcatccgcag gtggtcttgg cctgaggatt cttgatcgag aatcccgatc
     4321 cctgcacgct gtcgacatag tcgacctcgg ctccctgaag gagcggtgcg ctctgagagt
     4381 ccatgatgac tttcacgtcg cctttttcaa tgaccgtatc gtcctcggcc atcttggatt
     4441 cgaatgccat cccgtattga tagccgtgac agccgccgcc gcggacataa atccgcaaac
     4501 cgacaacgtc tttttcttct ttcatcaatt cgtgaatctt ctgttcggca accggagtaa
     4561 tcgtgaccat cgtgctcctc cttctcatca ggcgaaaacc gcccgttggc ttagtgtaac
     4621 acctctttac cgaatatcaa caccctgggc ttccgattgg gtcgtcggcg ctgcccactt
     4681 ggtttctggg acctttacca ccacgtcacc gaggattttt gcctggcacc cgagccggtg
     4741 ccaaggctcg ctaagagctt cccgatccag gagatcctgt tcttcaaagt cgatctcaga
     4801 gaggtgatca ctaccgcttt ggacctccac ccggcacgtc gtgcaggagg cgttgccccc
     4861 gcaatcatga ttgaggggaa agcccagctc ttttgccgca tcgagcaccg tcgtgttgac
     4921 cggaactgtc ccgctttttc cttttgggtg cagaaatgta accagcggca tccaccctcc
     4981 ttcagtgggc aacagcctca acaggaaccg acggatacgg acagcgttgc aatcgaatat
     5041 gttcctcata ctcgtgacgg aacagcttca cgctgctctg cacgagcacc gatgcccccg
     5101 tcaccagcgt acagtatccc gttcctttga cgaagccgca gagctgtaac aagctgtcca
     5161 gatccttttg agtgccctcg cctgcttcga ttttggtcat cagagtggcc agattaatcg
     5221 tgcccattcg gcacggtgga cattgcccgc agctctcgcc tttgaaaaag ttcgaaaact
     5281 tcaacgtctg tctgaccatg caggtggcat cgtcgacgac aatcaccccc gccgaaccta
     5341 atcccgtccc ggccttcttt aacgaatcaa aatccatcgg cagatcgagt tgatcggccg
     5401 tgagcatgga gaacgacggt ccgcccggaa acacggcctt gatcgttcgt ccgcccggta
     5461 cgccaccgcc gcactgctca atcaattcac gcacggtaac gcccatcggc atttcataga
     5521 cgccaggacg gttcacggcg ccgctcaatg agaacatcat cgtgcccgga cacttctccg
     5581 tccccacttg cgtaaaccac gccgcgccct tgtggagaat gcgggggata ttgcacaaag
     5641 tctccacgtt gttgaccagc gtcggtttgc cgtacaaccc gtaatcggtg ggataaaacg
     5701 gcggtttttg cctcggcatg gcagggcgtc cttgcatgga ctccaacatg gccgtttctt
     5761 cccctgccac gtagcttccg tgtccctcaa agacttggag gtcaatgtcg atgccgctgc
     5821 cgagtatatt cttgccgact aagccacgtt cctttgcctg agccaatgct tttttcagat
     5881 tttcacgctc ttcgtgatat tcgtgattga cgtaaatgaa agaggccttg gcctgtgccg
     5941 ttcgagaggc aatgagacag ccttcgatca gttgatgagg aattgttttc agcaggtacc
     6001 tgtccttaaa ggtgccgggt tcgtgctcac cggcattgca gacgaaatat cgttctttga
     6061 cccggtggtt cagaacctta tcccacttga cgccggtcgg gaaaccggca ccacctcggc
     6121 cacgcagtcc cgaagttttg agttcttgaa ccacttgatc tggcgtcagt cccttgacgc
     6181 acttcttcca ggcctcatag cctccgactt tgacataggc gtcaatgtcc cacggaggcc
     6241 catcggtctt ctcaatgagg cgtgcttcgg tcaaagtcat caagttcctt ccggccccag
     6301 agccaagaac ggtgaatggg tactataagg gccgcctcgg ccatccgtca aggtgaagtg
     6361 gccagaatag gcctcaatct caacaggtta ggacgagagc cctacccggc agtgggtata
     6421 atggccgata ggagaggcct tgactgatcg agaggacata tccggctggt ccttatacca
     6481 gccggagcaa aagaggctta tttcaagtgg ctgcccgcac ccatgaaaaa cagcatgggg
     6541 atcgaaagca gaaaattgac ccgagacgcc aataaggcgg tccggcccca ttgcgccata
     6601 tctgccgggg cgggcgtacc ttgcgccgct gcggtgaccg cagcaatgat cttcttttga
     6661 ttcggccaga taatcgcatg gacgttcacc atcataatga tgccgagcag cccaccgatt
     6721 cccagcgcat aggcaccggt tcccccgttc atgtacaact tgatgtagag aatgatcccc
     6781 gccagcacgg tcaccgtcgc tccatgccgg aaccagttca acgcggacgg catcaatttg
     6841 gggatgacga tattcttcgt gggaccttct aagcttttca gaaatgcggc attgatcagg
     6901 ttgaagaaat acagcaagcc gatccaggtg ataccggcca ggaaatggat ccagcgaatg
     6961 acgattccgg tccaatccac ctcgccggct ccaatgcctg tcatgccgag gtataagaca
     7021 atcaagacga gcgccagcgc gaaacctgcg cccatggtct gcatcggatc ttcaagaaat
     7081 ttcatagcat ctcctcattc ggtgcacagc taagccgctt ctgtacgtga gcccttcctg
     7141 cttgtcaagc gagtcagggc ctaggcattc gttgcattca gttcgtcatc acgcggtcaa
     7201 ccgtcgcgca cagttcgcgc acggcattcg ccgacgcttc gagcgccttc tgctcagccg
     7261 gagtcagatc atagaccatg atctgctctg cgcccccttt tccgagcttc accggaacac
     7321 cgacgacgac gttccgcagc ccgtattcgc cgtcacagaa gacggcacaa ggcatgacgc
     7381 gtttctggtc cttgtgaatc gcctcgacca tgtccacggc cgatgcggaa ggcgcataaa
     7441 acgcactgcc ggtcttgagc agtccgacga tctccgctcc gccgtcgcgc gtccgttgca
     7501 caatggcctc gagccggtgt ttcggcatga ggtctgaaat cggcttaccc ttgacggtcg
     7561 tataacgcgg cagaggcacc atggtatcgc cgtgcccacc caggaccatg gcttcaacgt
     7621 ccggagcggg aacgttgagt tcttgagcaa taaaggtgcg catgcgagca gagtcgagga
     7681 cgccggccat gccgatgatg cgcgacttgg gcaacccgct gacatttcga gcaacatgga
     7741 ccatggcatc caacgggttg gtcacgagga tcaggatgat gtcaggagaa cgcgagacca
     7801 gctccgtcac aaccgagctc acgatcttgg cgttggtcgc tagcagttca tcccggctca
     7861 tccccggctt gcggggcatt cccgacgtga ccacagcaat agaggacccg gccgattcct
     7921 cgtacccgtt gcttccgacg actttcgtgc tatagccgca gacgggaccc gcctgagaga
     7981 tatcgagcgc tttgccttga ggcacgcctt ccacgatatc caccagcacg acgtcgtagg
     8041 catctttttc cgccagccgt tgcgccgttg ttccaccgac attgcctgct cccaccacgg
     8101 tgattttcgg tcgccccata cgccctccgc ccacctctct acagcccctg gatgctcaca
     8161 gccgccgcca gaagccggcc acgaatgtcc gctcctagtg ttcgtgttcc gtccaccctg
     8221 ccactttgaa gttgatcgtg catacgaaat tttcgccgtg ataaaagttc accttgattt
     8281 tctttttgcc atacgttttt gccagaaagt cttcagggtt gtctatgagt tcttgatacc
     8341 cccccggcgg gtactcgccg agatcgtgaa acaccacaat cattcccgtt ttcacttcct
     8401 gcaggatctt gacggagcac tgagggtaca cctcccaaaa cttggcttcg atcatggcct
     8461 ttgtcggttc cagccgctct tcgcccggtg tgaagcgctg ggcaaacttg gccaaccggc
     8521 gcacataggg atactgatgg ccggtaaaca gcttatacaa ccagggaggc acagacctat
     8581 gttcaggttg atcggtcatg cttccgttac ggtgattctt gtcatcaaca tccaacattc
     8641 gacactcgtt ccattcactt ttgccgttag gtagcgaacc gccggtaaat gcgcggaagc
     8701 ctggccggaa gagactctac tcggtcgatg accgtgtagt gcacgtcacc atacatccgc
     8761 cgcatatacc cgtcggcttc gcgatcgatg gtgacgcaga acggatggat gccatgtcga
     8821 ttggcctcgc gtaatgccgc cttcgtatcc tcgagggcgt actcctcctt ataatcgccg
     8881 tcgagcggcc gaccatcgct caagagcagg agaagacggg tctttgcctc tcgcctcatc
     8941 agggcggcca ctgcatggcg aatggctgcg ccgtcgcggt tctgttgtcg gggactcagg
     9001 ccgcccaacc gttgtgcggt ggcgggaccc aatcgctcgt caaaaccctt gatcatcagc
     9061 acatccaccg accctcgtcc ctggccggaa taggcataga gcgcgtattg atcgcccact
     9121 gcctccaacg cctcacagag caggacgaga ctgtcccttt ctacgtcaat cacgcggcgg
     9181 ccgccttcga gctggcgtcc ggtcgatcca ctcacgtcga ccagcaatgc gaccgcgaca
     9241 tcgcgatctt ttttttctcg cctgacgtag acccgatcgc tcccttcgaa gcccgcggct
     9301 tgctcggccg ctcttctcac gaccgcatca agatccaggt cttcaccgtc ggcctgtccg
     9361 gccagacggc gaaaggccgg cggccgcaag ctttcaaaga atcgtcggag cgacctgatg
     9421 tcactccgat gtgccgtcaa ggtggccgac accgtttcat ctgagccgct gtccgccgga
     9481 cgctccagca cgcgacacca gttcacacga tagtcctgaa gcatgtagtc ccactcggga
     9541 tagcgaatcg tctgcccgcc tggcacggcc gattcgtggc tcaattgagg ttggacatct
     9601 tgggtcagga gttcctcaac gacggccggc aaggagtgcc cgccgccaag caggtcacca
     9661 ccgagcgaat gttcttctcc gaatcgacct ctctccgcgg gtatccgttc tattgagccg
     9721 cctccgggcg gctggacctc cgagtccgac ttctcgacat ctcctccgat catttccggg
     9781 tgcatctccc cccgataaaa cgcattgctc acaggacgat actcattgtc tgcctgttct
     9841 ggggcgcttt ggccgctggc agtttccgtc gaatcgtcgg ccggctgttc cgactcaagc
     9901 aggtccgcac gggaggccaa tagttcttc
//
LOCUS       DASSCQ010000055         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1129847, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000055 DASSCQ010000000
VERSION     DASSCQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129847"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /gene="ybgF"
                     /locus_tag="VFH05_03945"
     CDS             <1..482
                     /gene="ybgF"
                     /locus_tag="VFH05_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET6677393.1"
                     /translation="GILACLVLIAGQPTGTLAAPAQVQDTSRHLYDRVMEEFKHRDYE
                     AALAGFRLFLELHGQTSLAANAQYWVGECQFRMGRYKEALNAFYNVVSYYPLSQKLAA
                     STLKIGQTYTRLKDHEKARMMFERVVEQYPDSPEAEVARKAIEVEAAKIEPPLPPSE"
     gene            complement(499..1026)
                     /locus_tag="VFH05_03950"
     CDS             complement(499..1026)
                     /locus_tag="VFH05_03950"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HET6677394.1"
                     /translation="MRQAAIDTPFFALIAAVLVFLFWRIVSGWSDKAHPPGPFTLPLG
                     VVLRPQPLLAEKELLLYNLIRLAVEDRYLVFAQVPLWKFLMVEAEGDARIKVLRHLAL
                     KRADFVLVHPGSRVVEQVVQLDEESPTDPDRSANGREVQRILHSAGIRVTTLKAQPSY
                     TVQELEQILGVSDLE"
     gene            complement(1023..2198)
                     /locus_tag="VFH05_03955"
     CDS             complement(1023..2198)
                     /locus_tag="VFH05_03955"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET6677395.1"
                     /translation="MKIVGLMSGTSGDGVDAALVEVSGRGLTLRARALAAQTLSYPRS
                     LQQRILAASVSGTVAEICHLNALLGEWFADAALNVIRQAKLSPADIAVIGSHGQTVHH
                     LPQGIHAAGAGSIRSTLQIAEPAVIAERTGITTVANFRPRDMAAGGQGAPLTPAVHAL
                     LLRHPRRSRLIVNLGGISNVTYVPRGGEWSGVLAFDTGPANMVLDGLVNRATNGRVAM
                     DRDGKLALKGKADPRLLGKLLAHPFLSRRPPKSTGREAFGPSLIDELVSIQKQQGLST
                     EDLLATCSAWTAKAVGTARRWIKGTIDEVVVGGGGVRNRAVMDQLTAVFGVVPVTTFD
                     SLGWDSKAFEAVAFAVLAYRTVTSQPGNIPSVTGAKHPVLLGAIVPAAPGWMKHFRS"
     gene            2338..3264
                     /locus_tag="VFH05_03960"
     CDS             2338..3264
                     /locus_tag="VFH05_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET6677396.1"
                     /translation="MFFGPFWTWSCAILLTSSLTCAVSVAVAASSEPVNVVVTIPVLK
                     DLAEQVGGPYIRVTSLLKGYENEHTYSPKPSDLIAVRKAKLLFEVGLGLEVWVATLVK
                     NAGSASLMTITTSKGIGVIRDDQEPVAAHHSLGHEQGNPHVWMDPENAATMLRHITDA
                     LIEVDPDHAHQFRQNQAAYLVRLDRIRAELSERVRRLPDRRFVAHHPAWPYFARRFGF
                     EIAGTIQAQSGSEPSAIHIQQLIGTIKKQRIRAVVSEIQLSQKLPELLAKETGVRIVV
                     LTTLPGGLPQTETYLDMLRYNVLQLANALEAA"
     gene            3318..4112
                     /locus_tag="VFH05_03965"
     CDS             3318..4112
                     /locus_tag="VFH05_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET6677397.1"
                     /translation="MPASSDPIIQFDHATFGFPGVIALKDLTLAIQAGEFIGVIGPNG
                     SGKTTLCKAVLGLMAPVEGHLRIFDCACDELRCHHRAKIGYLPQKGVVDRNFPVTVLE
                     TVMMGRYGAMGLLKRPGTTDRLIAMEALAHVGMERHKNTALGHLSGGQQQRVFIARAL
                     AQRPQVLLLDEPTTGLDMTTQHNVIELIEHLHQELRLTVLLITHDINMIRSRVDRLIL
                     LKTRLYAAGPPGEVLKADILREVYGKDLVITDKDLIIVEDYHHHHH"
     gene            4129..>4311
                     /locus_tag="VFH05_03970"
     CDS             4129..>4311
                     /locus_tag="VFH05_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET6677398.1"
                     /translation="MLELLSYDFMQRSFLAAAMVGGLCSVIGVFVVLRGLAFVGAGTA
                     HAAFAGVALAYLIGWPP"
BASE COUNT          934 a         1262 c         1232 g          883 t
ORIGIN      
        1 tgggaattct cgcatgtctg gtcctgatcg cgggacagcc cacggggacc ctggccgctc
       61 cagcacaagt tcaggacact tctcgtcatc tttacgaccg cgtgatggaa gagttcaaac
      121 accgggatta tgaagccgca ctggccgggt ttcgtctgtt tctcgaactg cacggacaaa
      181 cctcgttggc cgcgaatgcc cagtattggg tcggtgagtg tcagttccgt atgggacggt
      241 acaaggaagc gttgaacgcg ttctacaacg ttgtgtccta ctatccgttg agtcaaaaac
      301 tggcagcctc gacgttgaag atcgggcaaa cctacacaag actcaaggac cacgaaaagg
      361 cccggatgat gtttgagcgg gtggttgagc agtatcccga cagtcccgaa gccgaggtag
      421 ccagaaaagc cattgaggtg gaggcggcca aaatcgagcc cccattgcct cccagtgaat
      481 gagtgccggt ccattcagtc actcgagatc actcacaccc aaaatttgct cgagttcctg
      541 aaccgtgtag gagggttgcg ctttcaacgt cgtcacccgg atgccggcgg agtgaaggat
      601 ccgctgaact tcgcgaccgt tcgcggatct atccggatcg gttggagact cctcgtccaa
      661 ctgcacgacc tgttcaacca cacgcgagcc cggatgcacg aggacaaaat cagcacgttt
      721 gagggccagg tgtcgcagca ccttgatccg cgcgtcgcct tccgcttcta ccatgagaaa
      781 tttccagagg ggaacttgag cgaacacgag gtaacggtcc tcgacggcga gtcgaatgag
      841 attgtagaga agtaactcct tctcggccag cagcggctgg ggccgcaaca caacccccag
      901 tgggagagta aatggtcctg ggggatgtgc cttgtctgac catccggaaa cgatccgcca
      961 aaacaggaat accagcacgg cagcgatgag tgcaaaaaag ggtgtatcaa tcgcagcttg
     1021 cctcatgagc gaaaatgctt catccatccc ggggcggccg gcacgatagc acctaacagc
     1081 acaggatgct tcgctccggt cactgaagga atgttgccgg gctgactcgt caccgtccga
     1141 tacgcaagca cggcaaaggc cacggcttcg aacgctttgc tatcccatcc caatgagtca
     1201 aaagtggtga ccggaacgac cccgaacacg gcggtcagct gatccatgac cgcccgattg
     1261 cgtacaccgc cgccgccgac caccacttca tcgatagtgc ctttgatcca tcgccgggct
     1321 gtgccaacgg ctttcgcagt ccatgcactg caggtcgcca ggagatcttc agtcgacaga
     1381 ccttgttgtt tttggattga gacgagttca tcgatgagtg aagggccgaa ggcttctcgg
     1441 cctgttgatt ttggcgggcg gcgagaaaga aagggatgcg caagcagttt gcccagcagt
     1501 cgcgggtccg ctttcccttt cagggccagt ttaccgtcac ggtccatcgc gacacgtcca
     1561 ttggtcgctc ggttgacgag gccatccaac accatattgg ccgggccggt atcgaacgcg
     1621 agaacgccgc tccattcccc tccacgagga acataggtga cattgctgat cccgcctaga
     1681 ttaacgatca gtcgggagcg ccgtggatgt ctcaacagca aggcatgaac tgccggcgtc
     1741 agcggcgcgc cttgcccacc agccgccata tcacgcggac gaaagttcgc cacggtggtg
     1801 attcccgtcc tttcggcaat gacggccggt tccgctatct gaagcgttga acggatcgat
     1861 ccggcgccgg ccgcgtgaat tccttgtgga agatgatgca cggtttgacc atgtgagccg
     1921 atcaccgcga tgtccgccgg gctcaacttc gcttgacgaa tgacgtttaa tgctgcatcg
     1981 gcaaaccatt cgcccagcag ggcattgaga tggcaaatct ccgcaacggt acctgagacg
     2041 gatgcagcga gaatccgttg ctgcagcgat cgagggtagg aaagggtctg cgccgcaagg
     2101 gcccgggcac ggagcgtgag ccctcgaccg gaaacttcca caagcgctgc atcaacgcca
     2161 tcaccggacg tccctgacat caggcctaca atcttcattc ggctcctcgt ctgatcgata
     2221 ccaccgtggg actgctacgc taatggaact ccggcgtctg gtcaagccgc acacagacca
     2281 attggaccat gcggacgcgt tgacatccaa gaaacctcga tgctacggtc cccgcccatg
     2341 ttcttcggtc ctttctggac gtggtcctgc gcaatcctgc tgacttcctc gctgacgtgc
     2401 gctgtttccg tcgcggttgc ggcatcgtcc gagcccgtga acgtggtcgt cacgattccg
     2461 gtcctcaaag atctggccga gcaagtagga ggcccctata tccgcgtcac ctcactgctg
     2521 aaaggctacg agaatgaaca cacctattca ccgaagccga gcgatctcat cgccgtacga
     2581 aaagccaaac tgttgtttga agtcggttta gggctcgaag tgtgggtggc cactctcgtg
     2641 aaaaatgccg gcagcgcctc tctgatgact atcacaacct ccaaaggaat cggcgtcata
     2701 cgggacgacc aggagcctgt cgcagcacac cacagcctgg gacatgaaca gggcaatccc
     2761 catgtctgga tggaccccga gaacgctgcg acgatgctcc ggcacatcac tgatgcactg
     2821 atcgaagtgg atccggacca tgcgcaccag tttcgtcaga accaggcagc ctacctggtg
     2881 cgactggatc gcatccgggc tgaactgagc gagcgcgtcc ggcgactgcc ggacaggcgt
     2941 ttcgtggcgc atcatcccgc ctggccgtac tttgccaggc gtttcggatt tgagattgcg
     3001 ggaaccattc aagcgcaatc aggtagcgaa ccttcggcca ttcacattca acagcttatt
     3061 gggacgatca agaaacagcg gattagggcg gtggtatcgg aaatccaact gagccagaag
     3121 cttcccgagc tcttggcaaa ggaaacgggc gtccggattg ttgtgctcac cacgcttccc
     3181 ggcggtcttc cccaaacgga gacgtatctc gacatgcttc gctataatgt gctccaattg
     3241 gccaacgcgc tcgaggccgc ttgatctggg ccgcccggtg cgatcgtttc ccggagcgcc
     3301 ggagggacac accgctcgtg ccagcatcct cagaccctat cattcaattt gaccacgcga
     3361 catttggatt tcctggcgtc atcgccttga aggatcttac tctggcaatt caagcggggg
     3421 agttcatcgg ggtgattgga ccgaatgggt caggcaagac cacgctctgc aaagccgtgc
     3481 tcggcctgat ggcgcccgtt gaggggcatc ttcgcatctt cgattgtgcc tgtgatgaat
     3541 tgcgttgtca ccatcgtgcg aagattggat atttgccgca gaagggcgta gtggatcgga
     3601 atttcccggt gacggtgctc gagacggtca tgatgggacg ttatggagcc atggggttac
     3661 tcaaacggcc cggaacgacg gatcgtctga tcgccatgga agcgcttgcc catgtcggaa
     3721 tggaacggca caaaaatacg gcgttaggcc atttgtccgg aggccaacaa caacgggtct
     3781 ttattgcccg tgccctggcg cagcggccac aggtgttgct gctcgacgag ccgacgacag
     3841 gtctcgacat gacgactcaa cacaatgtca tcgaattgat tgagcacctt caccaggaat
     3901 tgcgcctcac ggtgctgctt atcactcacg acatcaacat gattcgctca cgagtggatc
     3961 gcttgatcct gctgaagacc cgcctctatg ccgccggtcc accgggcgag gtgctcaaag
     4021 ccgacatcct ccgtgaagtg tacggtaaag acctcgtgat caccgacaag gatttgatca
     4081 ttgtcgaaga ctaccatcac catcaccact agaaatcaat aggaagccgt gctggagctc
     4141 ctctcctacg acttcatgca gcgctcgttc cttgccgcag cgatggtcgg agggctctgc
     4201 tcggtgatcg gcgtctttgt cgtcttgcgc ggactcgcct tcgtcggggc agggacggcg
     4261 cacgcggcat tcgcgggcgt ggccttggcc tatttgattg gctggccgcc g
//
LOCUS       DASSCQ010000056         7943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_595210, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000056 DASSCQ010000000
VERSION     DASSCQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595210"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VFH05_03975"
     CDS             <1..1102
                     /locus_tag="VFH05_03975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET6677399.1"
                     /translation="TFRLLFESIQDYAIFVMNPDGIVKTWNRGAEMMKGYRADEIVGQ
                     SFSRFYLSSDIEAGKPHMLLSHAARAGRVTDEGWRVRKDGSHFWASIVITALYDQQGA
                     LTGYAKITQDMTKRKQMEDELQDTLFLLEQGNKKLEELDQLKSLSISVATHELRSPLT
                     AVKAYIDNLFEGVAGELPEKIVHYLTRIGYNTDRVIRLVNMLLDISRIEAGHMHLELE
                     RVSVSEVICDVVKDFECMARTREIAIRASAVMDAPVRADRDRVEQVLHNLLHNALKFT
                     PPGGEVVVQSHLTDNEKVTITVTDTGCGIAPGHEEKVFHKFHRAPSPVREGAGLGLAV
                     TKSLVELHGGNIWVESEPGRGSKFSFTLPLVA"
     gene            complement(1936..2307)
                     /locus_tag="VFH05_03980"
     CDS             complement(1936..2307)
                     /locus_tag="VFH05_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677400.1"
                     /translation="MRFRGGLSAGLFFLSLSFLAPLPSLADTTDKTAKETEKQKMEME
                     REAQKQRHRMERQAQKDKHALEREEHKQKGELLGEEEKLQREQRKHKEEMAREEERHQ
                     TEMAREEKKHRQEMEKGGQTN"
     gene            complement(2424..2903)
                     /locus_tag="VFH05_03985"
     CDS             complement(2424..2903)
                     /locus_tag="VFH05_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6677401.1"
                     /translation="MAPQVQPIPPGYHTVSPHLAVRGGLEAMEFYKKAFSAEERGRFM
                     TPDNTRLMHGEIKIGDSIVMLGEENPQKGCPGPQSLGATTVSLFLYVQDADLVFNQAL
                     AAGAKSVMPVADMFWGDRAGTVSDPFGHQWTIATHKEDVPPEEMKRRGQAFFEQMAG"
     gene            complement(3118..3327)
                     /locus_tag="VFH05_03990"
     CDS             complement(3118..3327)
                     /locus_tag="VFH05_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677402.1"
                     /translation="MSQQKPITERIFEFLRGAPQGDFEGLVARYPEFTWNELYLEVSR
                     LNRQGLIKITRGVGIFTIQETPAVP"
     gene            4191..4454
                     /locus_tag="VFH05_03995"
     CDS             4191..4454
                     /locus_tag="VFH05_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677403.1"
                     /translation="MNQEQFGQFWEQLRTPLKAKWEKVTIEDLDEIQGDLVKFGSVLQ
                     RRYGESHTDEVTTWANRRYSHWTGNYVGYLDAEEFLSLHKEVV"
     gene            5257..5538
                     /locus_tag="VFH05_04000"
     CDS             5257..5538
                     /locus_tag="VFH05_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677404.1"
                     /translation="MSRLNMDPLLTFHDGSHLVMSTQCSKEGSFSCALYTVLISADDG
                     AAYRIISSHLEAQTCMRAQAIAYEHALHLYPQRSDLKQPPYLIWHGPRS"
     gene            5789..6097
                     /locus_tag="VFH05_04005"
     CDS             5789..6097
                     /locus_tag="VFH05_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6677405.1"
                     /translation="MGSKLYVGGLPYAATETEVTTLFAAHGTVESARVIADKFTGQSR
                     GFGFVEMSTPQEAQAAITALNGSQMDGRSLTVNEAKPMEARSGGGGRFSEGGRGRNRF
                     "
     gene            complement(6569..7099)
                     /locus_tag="VFH05_04010"
     CDS             complement(6569..7099)
                     /locus_tag="VFH05_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HET6677406.1"
                     /translation="MIMLLTHQEELRRQLTQALHVRGHKVAIPQHRDDMLTVLEDAKP
                     ELVILDLYLSDRSGAENLRLIHDHRYAGAVLVLSGPSMMPIVNVVYASGLRVTRVVQA
                     PVNVNGQYDFGDLEFNIQTLIDEVGNRKKSHASIARRAYELYEIGGRREGQNVQDWLR
                     AEQELSGSLGMRIGAR"
     gene            complement(7285..7740)
                     /locus_tag="VFH05_04015"
     CDS             complement(7285..7740)
                     /locus_tag="VFH05_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677407.1"
                     /translation="MQTLDIHRPEMPDLQFVLLVTALCTSRLTALNIPYTLRTAIFDR
                     CWVLIHDSPPPRRLEARVLDLRPWTEIAVEAMAETIRAGLTDAGIHTLAWDHPPSEPT
                     QPGTPDAQNLIDRLTRYASHARDPASGETVDEIGNSPDEEGIGGTASAR"
BASE COUNT         1702 a         2198 c         2139 g         1904 t
ORIGIN      
        1 gacgtttcgg ttgctgtttg aaagcatcca ggactatgcc attttcgtga tgaatcctga
       61 tggaatcgtc aaaacgtgga atcgcggcgc cgagatgatg aaaggctatc gagcggatga
      121 aatcgtcggt cagtcgttct ctcgttttta tttgtcgtct gacatcgaag cgggaaagcc
      181 acacatgctg ctctcccacg ccgcacgtgc cggccgagtc accgatgaag gctggcgtgt
      241 ccgaaaagat ggctctcact tctgggcttc tatagtcatt acggcgctgt acgatcaaca
      301 gggagcatta acgggatatg cgaaaatcac acaggacatg accaagcgaa aacaaatgga
      361 ggatgagtta caagacacgc tgtttctcct ggaacagggc aataaaaaac tggaagaact
      421 ggatcaactc aaatccctct caatttcggt cgcgacacac gaattgcgct ctccgcttac
      481 cgccgtcaag gcctacattg ataacttgtt cgaaggcgta gcgggcgaat tgccagagaa
      541 gattgttcac tacctcactc gcatcggtta caacacggac cgtgtgatcc ggttggtgaa
      601 tatgttgttg gacatctccc gcatagaggc agggcatatg cacttggagc tcgaacgcgt
      661 gtcagtgtct gaggtcatct gcgacgtggt aaaagatttt gaatgcatgg cgaggaccag
      721 ggagatcgcc attcgcgcct cagcggtcat ggacgcgccc gtgagagctg atcgcgacag
      781 agttgaacag gtattgcata acctccttca caatgcgctc aaattcactc ctccgggcgg
      841 cgaggttgtc gtgcagtcgc atctgaccga taacgaaaaa gtcaccatca cggtcacaga
      901 taccggctgc ggcatcgcgc caggccatga agagaaagtg tttcacaagt tccatcgtgc
      961 tccgtctccg gtgcgggaag gcgccggcct gggcctggcg gtcacaaaga gtctcgtgga
     1021 gttacacggc ggcaacatct gggtggagag cgagccgggc cgcggcagta aattctcatt
     1081 tactctgcca ctcgtcgcct gacccttaac agaactttaa cctgatccga acgtggcgaa
     1141 tctagcgttc actcgaccgt tatggtaaga gcagcctatg cagaaatgcg ggcgctgcgg
     1201 tggggtcctg cttcaggatg tgctcgagga ggtgggagtg ccattgcatg ccctgcattg
     1261 catggtgtgc ggcgagtggg tcgatccgct catcctccat catcgaaagc ttgccgtcct
     1321 tcctgaacca agccgagcac gcacaaccat cttcgatgcg gccacaagtg aaagatgctt
     1381 ggaacggcgc tgagcgccgt catcgttata ttgacccaaa gttatcctcg cctgtcccgg
     1441 tcgccgtcac tgccacctct cagcgtttca tgcccaacgc aattcgtgaa tacctctccc
     1501 gactcttctc ttcgggttcg gcagcgtact gtccaacatc aatggcgttc tgaaaggagc
     1561 cacatgagcc tcgagcgatt ctgcaacgga tgtggaaggc agcatggcag cgcagagtgg
     1621 tattaccgaa accgcgttaa ctcaaccgct cacgagtggt tatgcagtct tcaatatctc
     1681 cgccttccac cacaaaccat gtggtcatgg cgcacattct tgtattggaa caagtaaatc
     1741 aggttaagaa ggcgaggtcc gtccagccgc caaaaggtat ccattttgac acgaggtcag
     1801 ccttcgagaa ggtacctcgc gcctccgttc caaggcgctc ttcgcgtggc cgttttgtgg
     1861 cattcgtgag gcggtctgtg gttgggactt gctgaatatg aaatggtctt gagattaccc
     1921 ggctaaagtt cacgttcaat tagtctgtcc gcctttttcc atttcctgtc tatgtttctt
     1981 ttcctcgcgt gccatttccg tttggtgtct ctcttcttcc cgcgccatct cttctttgtg
     2041 tttgcgctgc tcgcgctgga gtttctcctc ctcccccagc agttctcctt tttgtttgtg
     2101 ctcctcacgc tccagggcat gtttgtcctt ctgtgcctgc cgttccattc ggtgccgctg
     2161 tttttgagcc tcccgctcca tctccatttt ctgtttttcg gtttccttgg cggttttatc
     2221 cgttgtgtct gccagggaag gcagtggtgc gaggaatgac agggaaagga agaaaagacc
     2281 cgctgacaaa ccccctctga atctcataag cgcctcctca aaaatgtccc tactcacaga
     2341 ttcgaccata cctccaggtt ccttggcgtg aagctagcga gaaccctagg aagaatgtgt
     2401 cgcgccgaat cgccctatgc cttttatcca gccatctgtt cgaagaatgc ctgacctcgc
     2461 ctcttcatct cctcgggagg cacatcctct ttgtgtgtgg caatcgtcca ctgatgtccg
     2521 aaggggtcag agaccgtgcc ggcgcgatcg ccccaaaaca tgtccgccac gggcattacc
     2581 gattttgccc ccgctgccag cgcttgatta aagactaggt cggcgtcttg cacgtagaga
     2641 aaaaggctga ccgtcgtggc gccaaggctc tgcggccctg ggcagccctt ttggggattt
     2701 tcctcgccaa gcatgacgat tgaatcgcca atcttgatct cgccatgcat cagccgtgtg
     2761 ttgtcaggtg tcatgaagcg gccacgttcc tcggcgctga acgctttttt gtagaactcc
     2821 attgcctcca ggccgccgcg aaccgccagg tgcggactga ctgtgtgata gccaggtgga
     2881 atcggttgta cttggggtgc catggtatcc tccgtcatgg tggatggctt ttgaggcgac
     2941 gaaataccgc attgtatctg agcggcgcaa atgaaaggat ctgtcaaaaa ctacagatag
     3001 ggatttcacc aggcagtctg tgagcagaca cggcacctta tagggtgcca gttgacgaac
     3061 cctgtgcagg ggaattgtcc gatcgaattt gaacgccagg gggtggggag caggtggcta
     3121 tggtaccgcg ggggtttctt gaatcgtaaa gatcccaaca cccctcgtaa tttttatcag
     3181 cccctgccgg ttcaagcggg acacttctag atagagctca ttccaggtga actccgggta
     3241 tcgcgcgacc aacccctcga aatcgccttg tggggcacct cgcaaaaatt cgaagatgcg
     3301 ttcggtgatt ggcttctgct gggacatcta gcctccttca ttccgctaat cgacccggga
     3361 atgccatgac acgccgagat ggattataca ctaacgttgc tgaataacgc gtccctggtt
     3421 caacggcgag gaataggatc accggagcgc atctggccgt cggcctgccg agtatctact
     3481 atgcagcttg acgcgactga cacccgtcga cgtgactcga gcttttcctc agcgactatc
     3541 gcgctttgcc tgacgccgtt tggtggttgc acggatgtga ccagcgatcg gtttagcacg
     3601 actctgcgaa agccgacggt tcgctcgcat gtccggggcg gattctgttc tctttgccgt
     3661 cttggggacc ttgaagccgc gaacagggga cggtggactt cccgcctttt ttgaccgctt
     3721 taatcgagcc atgaaccctc cttcatgggg gatgaattga ttccatagta tgacatatga
     3781 atgccatcat cggagcgggt atcggatttt tcatgtctcg tctcgaagcg ctgagcgctg
     3841 ctttccgctc ctcttcgact caccagtctc tccggcagcg catgggtgca ccgagaaatg
     3901 tatacttcat ataggataaa gatctgtcag cgcatgataa taaatatacc tgttggaatt
     3961 catagcttta gggtatcgcc cgtgtcctcg tctcattgac tcagcgcgct tcggggttat
     4021 gaagtatctg tcgagcacaa ctgactgttg cactccctcc gaaatcctca gttcgcgcgt
     4081 gtgatgctca ccaggtacac gcttctctag tggttttgcg gatccaggtc agaacaccgg
     4141 tccatggcaa caggaaccgg caaagatctt ttctaaccaa ggagcacatc atgaatcagg
     4201 aacaattcgg gcagttttgg gaacagctca gaactccgct aaaagcgaag tgggagaagg
     4261 tgacaataga ggatctcgac gagatccagg gcgatcttgt gaagttcggc tctgtgttgc
     4321 agaggcgata cggcgaatcc cacacggatg aagtgacgac ctgggccaac cgacggtatt
     4381 ctcattggac gggaaattat gtcgggtacc tagatgctga ggaattccta agcctgcaca
     4441 aggaggtagt atgagacgag attccgctat atctgttgca caagaaaagg gtcaggtctt
     4501 atcacgtcgc cggcgtggtg gcggactgac gtgtagtgaa gggcaggtag atgatgtgct
     4561 ctcaaaaggc gaatcgactg aagttgaagt gaagccgtct gtcacgaccg aaactctgtc
     4621 taccgcaact cctcaatcga cgtcggccgt tattcccgag gcgctcatgt cagcggaaat
     4681 tgatgagcag agcagactca tgaatattcg gaaaaagtga gccgacgtta gctgccgtcc
     4741 ggcgacattt tttggtgtat ccgccagtca ggatgtgtct ctgccacatt acgcattgac
     4801 attgtcctgt tatgcgccta aggtacattt ctcattcagc acaacctgcc gacggcctcc
     4861 tggaaggccc gaccgccgac attgtgagga aggcggtcgc aattatgtca ccctctcaga
     4921 ttctttccct gtgcgaattc cggaccggtc cattggacgt aggatctctt aaccgctgct
     4981 cttgatggtg catcatcaca gtccagtctc caactgcttt aggtcatacc tttaggtcat
     5041 acgatgcgaa agtctcgaaa aaccgttgca caagcaaagc gcaatggagc gccacctcaa
     5101 ccggccggcg aactgacgtg tagtgaagcg caggtggagg atgtgttctc ggctggggaa
     5161 tcgactgagg aggaggagcg catcgatcca cccaatcaac ggaagccgcc tcgttctcgt
     5221 taaaggcttt ctagcctgtc acaagaagga aaccctatga gtcgcctgaa catggacccg
     5281 ctgctgacct ttcacgatgg gtcgcacctc gtcatgagca cgcaatgttc aaaagagggt
     5341 agcttttcct gcgccctcta tacggttctg atctccgctg atgacggcgc cgcctatcgg
     5401 attatctcct cacatttgga agcgcagacg tgcatgaggg cgcaagcgat tgcctatgag
     5461 cacgcccttc acctgtatcc gcagagaagc gatctgaagc agcccccgta tctcatatgg
     5521 cacggacccc gttcataacc ttttgtctct cgcccaacga tgcgtgttgc taatatggga
     5581 aagaactgat acaagtaagc tactttcatt gcggcgggcc tcgcaagaaa gatgaaactg
     5641 agacggccca tcaccgatgt tgttgtcagc gagagtgtga tcggaagcct gacctaagcc
     5701 gcctctctat cgcgtcttcg gccctctcct tccctcgtaa ggtcgttctc gagtttccga
     5761 caatgtcatt tcagaaggag gaacccagat gggttcaaaa ttatacgtcg gcggtttgcc
     5821 ttatgcagcc accgaaacgg aagtcaccac cctatttgct gcgcatggca ccgtcgaatc
     5881 cgcgcgcgtg attgccgaca agtttaccgg ccaatcacgt ggcttcggct ttgtggagat
     5941 gtccacaccg caagaggccc aggcggccat tacggcgttg aacggctcgc aaatggacgg
     6001 acggtccctc actgtgaacg aggccaaacc aatggaagca cgttccggtg gaggcggtcg
     6061 gttcagcgaa ggtgggcgag gtcgtaatcg cttctaaagc gagatgactc gaggggcgtc
     6121 tgtcagacgc ccctcggcta cccaacgtct tctcaattct ctcctgacat cgtcacaaaa
     6181 tatctctcgc gttggacagt cgtacgatcg atatacaccg tccccggtct atcgtccgat
     6241 acacgacgac ttagatgtcg ttttgatctg ccgctgcctg agacgcttga gtctcgggcc
     6301 gacgatgcaa taatggctgg tccaatcgct ttgtgggatg ttcccgacac tctgcgtggg
     6361 cgaacttggc aagctgaccg ggtggcaatt ttaggactcg ttcaagttga tcatcggtgt
     6421 gtgttcggtt ggatggcgtt tggtgttgtg caacatagga ttctgccatg ttggtcgcac
     6481 gcgcgcgatc tcgtggactc aaccggagct gtttcagtcg gcggctcgtg aatagtgcga
     6541 gattcatgtg gctcctttgt gccggcatct accgagcacc tattctcata ccgagacttc
     6601 ccgaaagctc ttgctccgct cgcagccagt cctgcacgtt ttggccttcc cgtctccccc
     6661 caatttcata cagttcgtag gcacgtcgag caatcgaggc gtgggacttc tttcggtttc
     6721 ccacttcatc aatcagtgtc tggatgttga attctagatc cccaaaatca tattgtccgt
     6781 ttacgttcac tggcgcctgg acaacgcggg tgactcgaag gccgctggca tacaccacat
     6841 tcacaatagg catcatcgag ggccctgaca ataccagcac ggctccggca taccgatggt
     6901 catgtataag tctcaaattt tccgcaccac tgcgatccga taaatacagg tccaagatga
     6961 ccagctcggg ctttgcatcc tccaggaccg tcagcatatc gtcccgatgt tgcgggatgg
     7021 ccactttatg gccgcgcaca tgcaaggcct gggtaagttg acgtcgaagt tcttcctgat
     7081 gcgtaagtag catgatcatg taaaaagacc tccggagcag gctaccgccc gctcacttca
     7141 gctgacatcc aatggtcggc tgctcaatcc acaatgacac ggcgctttcg gattatgccg
     7201 ggaggctcgt ccctttgccc atgttctgcg ctcacagcaa tctacaatga agccggaccc
     7261 cgcttggcat tgaagtctgc catctcatcg agcgctggcc gtacctccga tgccttcctc
     7321 gtccggcgaa ttccctattt catcgaccgt ctctccgctc gctgggtctc tggcgtgtga
     7381 cgcataacgc gtaagtcgat cgatgagatt ctgcgcgtct ggtgtgccgg gttgtgtcgg
     7441 ctcactcggc ggatgatccc aggccagggt gtggatccct gcgtcggtca gccccgcacg
     7501 gatggtctct gccattgcct cgacggcgat ttccgtccag ggccgcaagt ccaacacccg
     7561 cgcctccagc cgtcggggag gcgggctgtc gtggatgagg acccaacacc ggtcaaatat
     7621 tgcggtgcgg agtgtgtacg gtatgttgag cgcggtcagc cgggaagtgc acagtgccgt
     7681 caccaacagc acgaactgaa gatcaggcat ctcgggacga tggatgtcga gcgtctgcat
     7741 ggttacacag cctgctttcc atggttcgcc aagaccattt gcatctccga ctcagccgag
     7801 accgagcaat gcttgatgac agcgccgcac acggacgctc cggcgattcg aaatgactca
     7861 cggctgagct gagcgcacga gccttacgtc aggttcacgt cgttccaatc gctgccgctg
     7921 gcttccggtt aaactccttg tat
//
LOCUS       DASSCQ010000057        27409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1760623, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000057 DASSCQ010000000
VERSION     DASSCQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 27409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27409
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760623"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFH05_04020"
     CDS             <1..220
                     /locus_tag="VFH05_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677408.1"
                     /translation="LLGPEMVGLMLPVLTWDGLFCMTKASSVLKVGTFKVDKNCHADK
                     LMHVYEEAAPPPFTSLRAVIPCDDRVCT"
     gene            435..1046
                     /locus_tag="VFH05_04025"
     CDS             435..1046
                     /locus_tag="VFH05_04025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6677409.1"
                     /translation="MIRSRLPRIENLKPSDQSKGFPLSLTFPIRANLKDGLPVQLVLA
                     GDRDVEPLRDLYRIIVEEGTSYPHERFPDHHDFMDYWFRGKSTVAAYAQDRVDTAPMA
                     GAFYLKPNWPGRARQVANAGFMVAPEWRKKGLGWLLGATMLEYAKQLGYRSVIFNLVF
                     SENVPARHLWEKLGFRQLGVIPGAVRKDSGAYQDAVIMFRSLL"
     gene            complement(1024..3684)
                     /locus_tag="VFH05_04030"
     CDS             complement(1024..3684)
                     /locus_tag="VFH05_04030"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET6677410.1"
                     /translation="MQIGWSRFWRLFITTPLLQAFVIGLLASVLATSLWSLAPVTSTT
                     LDWTLYDTWLRHRVPIATSPNLAIVVSDPQSEARLGTVLDRSILAQVVTSIHEAGAAA
                     IGIDHRLDRASPASLGGAASDALLLEAIETAGPLVFIHDPERALPSKGAITAHAQLLT
                     QPDHVTRLIPLLTVVQAESVPAFGSVLYDLYRQHDTPAGEPLMTRRASALINPVGNGA
                     LSALPTTALSSVWDALQHQDKERLEHQFRDKVVVILPHQSVQDSWLLPTGQSVPGIVA
                     HLHLLNSLLTDQQVTVTGPWAGTGLTLLLAACIAFGLLRFDGRVSLILAGSVLLLYAI
                     FSAALLWAIPLVLPLSLPLTAGCCVIVGTIVWGHLSADQRRLMLEQDMLRLQQDVSAV
                     REALILRQNRAEALEEDLETAKAAVMQSTAQQEEMAKSTASMRAQLVEAHMHEQAAQR
                     RLDELEQRLRDLRIADRASRTMDDAELDRLRRECREVGIVTQQPELLRMFHDLKRGAA
                     TPLTVLLLGEPGTGKELFARAVHRLSPRAQNAFIPVNMAAISPELFESELFGHTKGSF
                     TGAAVDRRGFFELAHRGTIFLDEIGDLRLDHQSKLLRVLQEKSFYRVGATNPTTVDVR
                     IVAATNRDLQRGVSEGWFREDLYFRLKGFVFRLPPLRDRRSDIALIAEATLTEIAQRM
                     GRPHPELSNDALGLLNSYRWPGNVRELRNVLERAVALSDGSILSRYAFSLEDVTIEDE
                     ERRDLAVALPDEAGDNAVLDRLRHHGFDMQATAKALSWDRSTVTQRLKGLCFQALVES
                     GGDRAKAVSAIAGDPARFRTVELKLNGYYDHLLSVIQPCKSVEEALAECKRRFKNIPD
                     RHFASVDRLVRTVFLQKPSNTSPSTAMTET"
     gene            complement(3688..4920)
                     /locus_tag="VFH05_04035"
     CDS             complement(3688..4920)
                     /locus_tag="VFH05_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677411.1"
                     /translation="MRRRLSALAIFSACAIASSFAQGPDDPTLLAPLESPPPPPEVVG
                     DEQARSPHTSVEPGHAAKRLAELRGLTRLSPRHAGERMALAEALYDTGDLDAAIAECR
                     LAVKLNPNSATAHSQLGILFMAKQDWRAAASVLTEAVRLDPTLTDAHYSLGSVYYSLG
                     ERKTAIQSYRQALALQPHFPDARYRLALLLMLSSQTQEAAQLMEEAAIGGVSQAQYFL
                     GNAYKSGQGVEKDLAKTIFWWMKGVEYGYQPAADSLSKLRRQALSADQPERKRKEALD
                     AFQRYRDKLWEEFHDYGSMEDGRTLGTTLLADGRIDVAVPVLLKEGYALSEVAQGALG
                     TLYESGSGTALKPYDTEILTCFETTAAEGFVPAKRLLARVYGRGIGVHQDLSKAKGIL
                     KGLPKTEVNAILTEIGGR"
     gene            complement(5010..5426)
                     /locus_tag="VFH05_04040"
     CDS             complement(5010..5426)
                     /locus_tag="VFH05_04040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET6677412.1"
                     /translation="MSDPPCDACAGVWPLADHFIADLGLSLAYLHDDQFFPGWTVLLF
                     KRHATELFHLAPTERIQLMEEVSRLTKVLAETFQARKMNYELLGNQRPHIHWHLIPRL
                     AHDPAPLEPVWRVPHDRIALSPEQVGTTISLIKKQL"
     gene            complement(5423..6049)
                     /locus_tag="VFH05_04045"
     CDS             complement(5423..6049)
                     /locus_tag="VFH05_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677413.1"
                     /translation="MRISHLATTLLIGMLLIVWAFNIGPADASPSEQLADLTGKASVS
                     VTLTGRDNFNSEYRYDLTVRNRSSDPLIADSLIIVLEKITNLAGEDREALKSEPLLNR
                     FEIVGQDGETEDGKPFFRVPVGASPDLAPLTDSLPLSIRIRNRDYVSVFTPSFRVYGK
                     KRPPAEPKPSVSQAQPAPSPQDRQTDRLIQLLLKKGLITEEERKTIQP"
     gene            6195..7055
                     /locus_tag="VFH05_04050"
     CDS             6195..7055
                     /locus_tag="VFH05_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677414.1"
                     /translation="MDTQRAPLLVSRSLVSRLMGLYDVSHPLHKRRVIRGYDRAHAVR
                     TARMCAAVASALGHGTERVRQYQIACLLHDLGRAGLDRRLFGKIWSWAKAHGIPTRPR
                     EWRAIHPETPYGRETEAFVARYREALEADGIPVTAWVKEQIEMRLGYARRLAKRLRAV
                     RSSLTQMGVRFEPWMQRVMLYYYYPEKLSRAQPWVKQLAEVLVACEQFEAYSNQRRGR
                     DYYVRSKESIAEAFAYLDKLEQERILSVEVMKVMKRLAAAGEFNGVIAAARGRRLTPQ
                     ERRFLRRMEG"
     gene            7058..7480
                     /locus_tag="VFH05_04055"
     CDS             7058..7480
                     /locus_tag="VFH05_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6677415.1"
                     /translation="MAVQTLPLRIDMRGGTQIENVTKFVQEALAHAKMSAGIVTVFVQ
                     HTTASVMVIEDEPGIRSDTKTFWDRIAPADQSWQHNEQNPGEDNGHSHLRGQLQGPSV
                     TIPFSNGSLLLGTWQQLVVVDFDTRARTRDLIIQILGE"
     gene            7498..8613
                     /locus_tag="VFH05_04060"
     CDS             7498..8613
                     /locus_tag="VFH05_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6677416.1"
                     /translation="MAGLLAVMLQLAGEGRLCDVAAADWGKPLGALYDGVEVRPRPVT
                     PAPTELGPDERATMAVFERATKSVVFISNTAIQRDFWSFDVLEVPQGSGSGFVWNKQG
                     HIVTNFHVIYGANTIKVTLADRSEHQAKLVGADPDHDLAVLQIQAAESLLEPLAIGSS
                     HDLRVGQKVLAIGNPFGLDHSLTTGVVSALGRTIKSLSNRTIEGVVQTDAAINPGNSG
                     GPLLDSAGRLIGVNTQIISPSGAYAGVGFAVPVDTVNRIVPELIKHGKLIRPGLGVSL
                     VPDAIVKRWGIKGLVIGRVTQGGAADRAGLRGARETPTGRIELGDIVVAVAGKPVTTI
                     DELMAVLEDHKVGEQVTVEILRGNRREKITVTLQAVN"
     gene            8673..10412
                     /locus_tag="VFH05_04065"
     CDS             8673..10412
                     /locus_tag="VFH05_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6677417.1"
                     /translation="MSDRRAPDSPQEPTSTREDPGTKTGSDPLELIEQCLALFPESDP
                     RQKLLYKLRHAVMLSQAGNQQREAEFRKVSEVVAKLTAPANRIGMLLGLPGEDLAHIL
                     VGGAEYYANVDPRVASGDLKIGTQILVNEAYAVIKTLGYDRNGPILKLTEAMADGRLR
                     FEQEMGRQSLILQRSSDLAGVELKAGDEVRIDSSLRIAIEKLADRKAAKHVLDEAPNV
                     TWDQIGGQQEAIAAIRKAIEYPLLHASTFEQFKFSQPKGFLLYGPPGCGKTLIGQAAA
                     ASLSKLVSESAPDIHGTGDARAPITGGAFLHVKGPEILNMWLGESERMVRDLFVQARA
                     KRLEGALPFIFIDEAESILGTRRAMRSFNISNTLVPMFCSEMDGIESLRDVVIILASN
                     RPDLIDPAVLRPGRIDRKIKVARPNREASAQILKVYLHHDLPLDPALLAERGGEQGRA
                     MASLAGDVLERIFKRSDENRLLSVRLRNGQQKVLYRGDLVSGAILASIVQRAKEKAID
                     RTIQSGQPAGLLAQDLFDAVTEEFREGEVLPPDDAAEEWLKLLDHHPEQVVGVSSFRR
                     GRQTEEQLVNQII"
     gene            10440..11978
                     /locus_tag="VFH05_04070"
     CDS             10440..11978
                     /locus_tag="VFH05_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HET6677418.1"
                     /translation="MWGSVIRLFGIETEYGIARDDLDTMDPVVESMELVRAHLTGPFE
                     RRWDYASEDPHEDARGFRVSGLAQDKEEAQFSSIDAHRPFSFHEMKSDLVLPNGARFY
                     NDHTHPEYSSPECRTLKDLVAHDRAGERIVQRAADRRNQRLGGTPVQLYKNNTDFHGH
                     SYGCHDNYLVSRSIPFPALVAGLLPFLVSRQVVAGAGKMGIEAESGFVPGTYQLSQRA
                     DFIEAELSVDTMHNRPILNTRDEPHADAKKYRRLHLIIGDANMCEYATALKVGTTQLV
                     LELIARGRAPRLELEEPVTAVKQIARDPDLKAAVRLKDRRNLSGLELQECYWETAQRE
                     LAGADPETDWILCEWGETLRLLIQHREQLVGRLDWVTKQWLLETFIREEQIGWDDPWL
                     ASLDLEYHNVNPERGLYRGLEAEGKVWRMTSDADVEEALARGPLDTRGGLRGICVRRF
                     ADQITDMQWERIRFSGGWRPKILDMGDLFDPLEVRRCVALFEEAASPADALEAWKTRK
                     DRTL"
     gene            11975..12169
                     /locus_tag="VFH05_04075"
     CDS             11975..12169
                     /locus_tag="VFH05_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HET6677419.1"
                     /translation="MTYDMLPERREGPINPMPKSPDPSEEGGGPRRPESGSPDKDNLM
                     KRMKKVDPKQAERYRQRTGQ"
     gene            12240..13013
                     /locus_tag="VFH05_04080"
     CDS             12240..13013
                     /locus_tag="VFH05_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HET6677420.1"
                     /translation="MGMQGDFFQLLKAQGYQFGAAGQAGNGQEFTTATTILAFKYRDG
                     VLVAGDRRATAGNMVMYDRTDKVLEIDRHSVMAIAGVPATAYEMVRILEHSFKYYRRT
                     QLQELSFEGKLRAVSKLLKDNVAAALAGTGAVAPIFAGYDFEQESAKIFFYDILGAEF
                     EGVEYAVSGSGSPTIRGILHYLNTWGDQPLHAMSEDQSTIQALRLLTSAAEFDSATGG
                     VNREANLYPVIKLITRDGVRTLPEAQLKQSFEASVSRFV"
     gene            13060..13866
                     /locus_tag="VFH05_04085"
     CDS             13060..13866
                     /locus_tag="VFH05_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="20S proteasome subunit A/B"
                     /protein_id="HET6677421.1"
                     /translation="MYEEPYKWVEAVGNRRHYLDEQFTQGSPVVALAYDAGILMLTVS
                     KGTPKLYEIYDRLALGGMGHPADLEKLRFSLLEMAHVEGFNRSPSDVTGSRMVKYGIA
                     PVIKQAFEEVFKAPFIVKILLAELGQKPGKDIFLVINYDGTFEEGPHCAVLAASKSVE
                     QDMLAYLRKSGTMQLPLDQAVGAALQAWALGDRSRRNALAAENVEGAAAGSPPPDAAG
                     LYAYVREAVAEKTLEGAVLDRTQPGSSKYRALTAEELGRLLPQDIKKSSL"
     gene            13871..15355
                     /locus_tag="VFH05_04090"
     CDS             13871..15355
                     /locus_tag="VFH05_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HET6677422.1"
                     /translation="MLKRIFGLETEYGLLVHQDRPDHSPTWFAHQIRDHVFHVQRQGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYLDMGHLEYASPECHSLTDLVASDRAGDTILQRA
                     VHDLGLGDQVSLIRNNVDHETDATFGSHENYLVTRRFPFSRRGLAPLVTFLVTRQIFT
                     GSGRVGAASPQDAWIQMDRLIVPRAALGHRSQSAQLPFQISQRADHIVNDFFEWVQQN
                     RAIVNTRDEPLADPNQYRRIHLLLGDSNMAEYATALKLGTTGLVLQLIEEGKGPRDLD
                     LDEPVEALQEISQDQDREWILRLQSGKRISAIDIQEQFLAAAKMHCTGQDDETDWVLE
                     QWEAVLQDLRGDYGCLVGRVDWASKLWLLENFREAEKLEWSDPMLKSLDLEYHNLNSE
                     KGLYYGLLQEGRVPRLTTDKAVELAMEHPPRNTRAFGRGELVKHLLTCGPPAEPEAAR
                     KEERLFPSYVINWSIFQLRGLEPFPMPDPFKSYAQEVRSHLVRP"
     gene            15474..15932
                     /locus_tag="VFH05_04095"
     CDS             15474..15932
                     /locus_tag="VFH05_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677423.1"
                     /translation="MKAKRWMMEYGVAMILAILLAVVLGHLPLFRDTAVGKLHASDVV
                     QFMGYGTGIILAWFGARQLAADPPEEWKWLVAYRSLILPVTTLILVGLAYHVLLYVSQ
                     PFLGKSGKETYNWTFIIGLIVCCGWLILTWLQKCAPQIASAEPRRLRKAA"
     gene            complement(15976..17007)
                     /locus_tag="VFH05_04100"
     CDS             complement(15976..17007)
                     /locus_tag="VFH05_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6677424.1"
                     /translation="MTEGKCQRTVNHARDAAAMELPAFVPARPLRNHHLMTVVPRYWS
                     RGPLLAGIPVERRLFRTEPDTQIVGFCHWQDNRWSAQTMLLLHGLEGCCDSHYMHGIA
                     AKAYKAGLNVVRLNQRTCGGTEHLSPTLYNSGLSADYRSVLQELAAEQMHRIWLVGYS
                     MGGNLLLKATGELGRSQPALAGTVVVCPNIDPTLCVRELERPANWLYHRHFLTRLKAR
                     VQRKASLSPGRWDLKGLRAITRISEFDDRYTAPDGGYRNAADYYDRSGARHVLHSIAV
                     PTTIITAQDDPFIPYSMFLTPSVQQNPSIRLLAPTHGGHCGFFQTRRSEEDRYWVENR
                     IVEIVRGRG"
     gene            complement(17017..18222)
                     /locus_tag="VFH05_04105"
     CDS             complement(17017..18222)
                     /locus_tag="VFH05_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6677425.1"
                     /translation="MNPPPMHLPDILLAITLLLTISAVMIPLARRVGIGPELGLLLSG
                     VVLGLPHLLAPAHVARLRELSELGVVFFLFVIGLELDLRQAWSLRRYALGMGVLQVAV
                     TGILLMLYWRWFSQSWSLGLLLGLVLANSSTALVIQILERKNELDQEHGQASFAVLLF
                     QDLTVVPIIALVPLLAGTGSTDVHSLWQVLPAVGAVALLFFVGRKICPWLFSLASEKH
                     MSETFTAVLFIAVLGSAWVASRMGLSMALGAFLMGVALSGTEQRHQLRNEVIPFKNLL
                     LGLFFVAVGLTVDVSVLTEHPVRILMHVLVIVVVKVAVLYLAARIIRMEHGPAARVAF
                     LLAQAGEFGFVILGTLLASNVITPAQFGNGIMVIALTTIMTPLLDSMGIAWSRLYRPV
                     MPGAKASTS"
     gene            complement(18478..18553)
                     /locus_tag="VFH05_04110"
     tRNA            complement(18478..18553)
                     /locus_tag="VFH05_04110"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18518..18520),aa:Asn,seq:gtt)
     gene            complement(18617..18886)
                     /locus_tag="VFH05_04115"
     CDS             complement(18617..18886)
                     /locus_tag="VFH05_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677426.1"
                     /translation="MGDEDNIIDELLKEISRLILEYPKAIERRAAVIQATGKDPELVE
                     KLVKAADTMRDSGNLYLTWAKHYAALAQGNTDASSDEDETEDFDV"
     gene            complement(18961..19239)
                     /locus_tag="VFH05_04120"
     CDS             complement(18961..19239)
                     /locus_tag="VFH05_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HET6677427.1"
                     /translation="MYNVAQVIDEKCTAKKGCRLCIMYCPEANCLDLNSAKMVAEVNI
                     DRCKGCELCVVVCNAAKHEAILMQAVSATGQLMSKKGESAALGQAYQG"
     gene            complement(19258..19965)
                     /locus_tag="VFH05_04125"
     CDS             complement(19258..19965)
                     /locus_tag="VFH05_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET6677428.1"
                     /translation="MIKKRLNIRMSGLGGQGAVTAAHVLAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPDVIQVFHPQVITMGKSYTMPFYSGVKEGGVV
                     VINSAQPLLSEEDVQRLKDLNVAVFYIAGTELAIEVAGTELSTNMAMIGSVSGITKCV
                     TMDALDGALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKRAYEIASEW
                     AEKNKVELQVGNPAVAA"
     gene            complement(19983..20885)
                     /locus_tag="VFH05_04130"
     CDS             complement(19983..20885)
                     /locus_tag="VFH05_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6677429.1"
                     /translation="MSKERIKISEDLYDIMPSDYQDLVKSATYGKEDRGWKDIGSSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPGRVKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVNCTVSKPSLVEKV
                     IRNAVHIAREIGPTYLQLYTPCILEIGKNSMEGLQEMRDSEKPAERFAYKEYVSEPAK
                     QLLAELAAKDKERKAAAKQLAGQA"
     gene            complement(20937..22148)
                     /locus_tag="VFH05_04135"
     CDS             complement(20937..22148)
                     /locus_tag="VFH05_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET6677430.1"
                     /translation="MADTHSVIGTQNKKGQTYTDTWKMMHEAPRTPSFFTGSEVIKEA
                     LRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTLEIAYLLNTGML
                     VWHAETAQDFFDWILKGYMVSEEPDVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFVMKSNYISYATHASWQQEIWAAIERSRKHSIHWL
                     NGLIDTEDIDQEIMIVASGTAVSQGREAIRLLEDEGVRCGLIKIKTLRPWPEEEIREA
                     TKNAKHIFVPEFNVTGWLAKEIRATIPNPHRVHAGPHVCGGMTMPPEIIVSEIKTTLG
                     MKSFSLAGRGS"
     gene            complement(22189..22824)
                     /locus_tag="VFH05_04140"
     CDS             complement(22189..22824)
                     /locus_tag="VFH05_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HET6677431.1"
                     /translation="MSQYRVLPGPEHFLPPAAASMGIFLPNPGEAHINGVIVSEEKAY
                     EEAARQFLMAKVPTIFPGPLVLWAWNEKAAKKATAIRHLYNTLKECVQPGQTAMLIPM
                     PDYRPKYPKINPEVEINPNHPNLTIWHNKIDCCMFVGVHCHQANLSLKIIRGGTSCYT
                     IAMCAQAGHEDAMLSFRDASVEKIMKLADTVKRLKGTVQPRQTSAKSGASN"
     gene            complement(23164..24426)
                     /locus_tag="VFH05_04145"
     CDS             complement(23164..24426)
                     /locus_tag="VFH05_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677432.1"
                     /translation="MRLWGVLALAISAFALFSGHPALALTRTVIAEAQYVMADGDTLA
                     TAEEKVLQRAQRRAVEEAGVYLESTFYDVEQESYGRTTRNTSLEIRTIAAAITETEIL
                     DSRRSFENERPVFSIRIRASVDLHNLAEAIRRLQSEAQLARHFRELQLENRRLRAQLE
                     DLRQQPSGVRVLSIDASGKPEPVQRAKQQLELAFHTPDLEKKIELASDAARLDPQSVE
                     PLIVRGQAYLRLISIAYSEHQKSGEYAGHIEKARSDFDQAVELDVKNPWAWIGKGDVQ
                     TWLKRTDEASLAYERALALDPFFDLARQRLIGLYTTQARRQAKAKQWQQSLLTLHKLL
                     DAPVPDSWRPYQKEAFLLRSDVLLKLNRSEQALDDLSTVLLIDPTHVGALLTRAHLYQ
                     ARLQGRLAKDDFERACVLGSLKACNQLP"
     gene            complement(24503..24793)
                     /locus_tag="VFH05_04150"
     CDS             complement(24503..24793)
                     /locus_tag="VFH05_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6677433.1"
                     /translation="MKFVIIGFDGPEGEAKRKIYRPAHLANMEPLDQAGRVVLAGPLT
                     DKTGSLIVIEADSLEEAERFAREDPYTVHGVFERVEVHPFTQIFPKSTLPTQ"
     gene            complement(24813..25223)
                     /locus_tag="VFH05_04155"
     CDS             complement(24813..25223)
                     /locus_tag="VFH05_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET6677434.1"
                     /translation="MAGDEDQEHDRLFTLSEANHLIPQLQSRLASVRQAKAVLARTKD
                     DIRRASARAEYGGGSTVGPLYLSSLHQISSNVQAIQEMGVLVKDLDLGLCDFPHLREG
                     RVVFLCWKLGEQEIKWWHETTSGYKDRCPLEDSV"
     gene            25679..26926
                     /gene="rho"
                     /locus_tag="VFH05_04160"
     CDS             25679..26926
                     /gene="rho"
                     /locus_tag="VFH05_04160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET6677435.1"
                     /translation="MHLADLKQKTIADLNDVARDLKIEGAANLRKQELIFAILQAQTE
                     KNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNFEDPEVARDKILFDNLTPLYPEERIQLEFDREEYCTRVMD
                     LTTPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALVDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRIFPAIDISQSGTRKEELLVDKDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKVHGTKTNQEFLQSMNR"
     gene            27014..27220
                     /gene="rpmE"
                     /locus_tag="VFH05_04165"
     CDS             27014..27220
                     /gene="rpmE"
                     /locus_tag="VFH05_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET6677436.1"
                     /translation="MKKGIHPLYREATVHCACGNSYKTRSTVGDINVDICSSCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKGK"
BASE COUNT         5893 a         7588 c         8104 g         5824 t
ORIGIN      
        1 tttgttgggt cctgaaatgg ttgggctgat gttgccggtt ctgacctggg atggactgtt
       61 ctgcatgact aaagcctcct ccgttttgaa agtaggaacg ttcaaagttg acaaaaattg
      121 ccatgctgac aagctaatgc atgtatatga agaagcggct ccacccccat tcacctccct
      181 tcgagccgtc attccatgcg acgatcgggt ctgtacctga gcaatcggag tgccgatcag
      241 aaaagggcaa ataacttctg ggacggcagc aaaattcagc acttcagagg ggacacggtg
      301 tgcggggaaa cggctccctt catgcgctgc ggggctggcc ccgcatgcga gcatatccag
      361 agctacgctg ccttcccgca tcgctgagag cttcaaggca ggtcagaatc gtcaaggacc
      421 ctagcctctc gtctgtgatc cgaagtagac ttcctcgcat cgagaatctc aagccgagcg
      481 accagtcaaa agggtttccg ttgagcctca cgtttcccat tcgcgccaac ctcaaggacg
      541 gcttgccggt acagttggtc ctggccggtg accgcgatgt cgaacccctt cgagacctct
      601 atcgaatcat cgttgaggag gggacctcat acccgcatga acgattcccc gatcatcatg
      661 actttatgga ctattggttt cggggcaaga gcacggtggc agcctatgca caggatcgag
      721 tcgacacggc acccatggcc ggggcctttt acctcaagcc aaactggccg ggacgggcaa
      781 ggcaggtcgc gaacgccggg tttatggtgg cgccagagtg gcgtaagaag ggtctgggct
      841 ggctattggg agcgaccatg ttggagtacg ccaaacagtt gggatatcgg agcgttattt
      901 tcaacctggt tttctctgag aatgtgccgg cgcggcatct ctgggaaaag ctaggcttca
      961 ggcaattggg tgtgattccg ggcgcggtac gcaaagactc cggagcctat caggatgccg
     1021 tgatcatgtt tcggtcattg ctgtagaggg tgaagtgttt gaaggcttct gcaggaacac
     1081 tgtccgcact aggcggtcaa ccgacgcgaa atggcggtct ggaatgttct tgaaccggcg
     1141 cttgcactcg gccagggctt cttcaaccga cttgcatggt tgaatgaccg acagcaaatg
     1201 gtcgtaatac ccattcagct tgagctcaac ggttctgaaa cgggcagggt ctcctgcgat
     1261 cgccgatacg gctttggctc ggtcgccccc tgattcgaca agagcctgga aacacaggcc
     1321 cttgaggcgc tgtgtcacgg tgctgcgatc ccaggacagg gcctttgccg tcgcctgcat
     1381 atcgaatccg tggtgcctca atcgatctaa cacggcattg tcccctgctt cgtcaggcaa
     1441 ggcgacagcc agatcgcgcc gctcttcatc ctcaattgtg acgtcttcca gtgagaatgc
     1501 gtagcgagac aggatgctgc catcgctcag tgccaccgcc cgctcgagca cattgcgaag
     1561 ctcacgcaca ttacccggcc accgatagct gttcaggaga cccagcgcat cattggacag
     1621 ctcaggatga ggacgtccca ttcgttgagc aatctcggtg agcgtcgctt cggcaatgag
     1681 ggcgatgtcg ctccggcgat cacgcagcgg gggcagacgg aagacgaatc ccttcagtct
     1741 aaaatagaga tcctcgcgga accagccttc ggatactcct cgttgtaagt cacgattcgt
     1801 cgctgccaca atccgcacgt cgaccgtcgt gggattggtc gctccgacac gatagaaaga
     1861 cttttcctgg aggactcgta agagtttgct ttgatgatcc aggcgaagat cgccgatctc
     1921 gtcgagaaag atcgtgcccc ggtgtgccag ctcaaaaaat cctcgccggt cgaccgcggc
     1981 tccggtgaag cttcccttgg tgtgaccgaa cagctcgctc tcgaaaagtt ccggtgaaat
     2041 tgccgccatg ttgaccggga taaatgcgtt ctgcgcacgg ggactcagcc gatgcacggc
     2101 tcgtgcgaac agctcctttc cggtgcccgg ttccccgagg agcaggacgg tcaatggcgt
     2161 cgcggctcca cgcttcaggt catgaaacat tcgaagcagt tccggctgtt gtgtgacaat
     2221 tccgacttca cggcattccc gtctaaggcg gtccaattcg gcatcgtcca tcgttcggga
     2281 tgctcggtct gcgatgcgaa ggtcgcgtag tcgctgttcc agttcgtcga gccggcgttg
     2341 ggcggcctgc tcgtgcatgt gcgcctcgac caattgcgcg cgcatcgacg cagtgctttt
     2401 ggccatttct tcctgttgag cggtcgattg catcacagcg gctttggcgg tttccaaatc
     2461 ctcttccagg gcctcagcgc ggttttggcg caagatcagc gcctcgcgta ccgcgctgac
     2521 atcttgttgg agacggagca tgtcctgttc gagcatgagc ctgcgttggt cggcgctgag
     2581 atgaccccag actatcgtgc cgacgatgac acaacaccca gcggtcagcg gcagggagag
     2641 aggcaggacc agagggatag cccagagcaa ggctgcggag aagatcgcat agagcaacaa
     2701 cacgctgccg gccaggatca ggctcactct tccgtcaaag cgaaggaggc cgaacgcgat
     2761 gcaggcggcc agcaggagag tgaggccggt gcccgcccag gggcccgtga ccgtgacctg
     2821 ttgatcggtg agcaagctat tgaggaggtg aagatgggcc acaatgcccg gcacggactg
     2881 tcccgtggga agcagccagg aatcttgaac gctctgatga ggaaggatga cgacaacctt
     2941 atccctgaac tggtgttcca gccgctcctt gtcctgatgc tgtagagcgt cccagaccga
     3001 ggagagtgct gtcgttggga gcgcagacaa ggccccgttg ccgacgggat tgatgagtgc
     3061 cgatgcgcgt ctcgtcatca gcggttcccc cgcgggtgta tcgtgctgtc ggtagagatc
     3121 atagagcacg gaaccgaagg ctggcactga ttcggcttgc acaacagtca gcaaaggaat
     3181 cagccgtgtg acatgatctg gttgggtcaa gagttgtgca tgggccgtga tggctccttt
     3241 cgatggcaac gcccgctccg ggtcgtggat aaacacgagc ggtccggccg tttcaattgc
     3301 ttcgagcagg agggcatcgc ttgccgctcc acccagggat gccgggctgg cacgatcgag
     3361 acgatgatca atgccgatcg cggcggcgcc ggcctcgtgt atgctggtca caacctgggc
     3421 caggatagat cgatccaata cggttcccaa tcgggcttcg ctctgcggat cggagacgac
     3481 aatggccaag ttcggactgg tcgcgatcgg gactcgatgc cggagccagg tatcatagag
     3541 agtccaatcc agggtggtag aggtgacggg agccaggctc caaagactag tggccagaac
     3601 cgacgccaac agaccgatga cgaacgcctg taggagggga gtggtgatga agagccgcca
     3661 gaatctggac catccgatct gcatgcgcta ccggccgcct atctccgtaa ggatggcatt
     3721 cacttcagtc ttgggcagtc ctttcaagat gcccttggcc tttgaaaggt cctgatgaac
     3781 gccgatgccc ctgccgtaca cacgggccaa taatcgcttg gcggggacaa agccttccgc
     3841 cgcggtggtt tcgaaacagg tcaaaatctc cgtatcgtag ggcttcaagg ctgtgcctga
     3901 gcccgattca tacagtgttc ctaatgcgcc ttgtgccacc tcgctcaagg cgtagccctc
     3961 tttgagcaac accgggacag caacatcgat acggccatcc gcgaggagtg tggtccccag
     4021 cgtgcgaccg tcttccatcg acccatagtc gtgaaattcc tcccacagct tgtcgcgata
     4081 ccgttggaag gcatccagtg cttccttgcg ctttctctcc ggttggtctg ctgagagcgc
     4141 ttgtcgtcga agcttggaga ggctatcggc agcgggttgg tagccgtatt cgacgccctt
     4201 catccaccag aaaatggttt tcgccagatc tttctccacc ccttggccgc tcttgtacgc
     4261 gttgccgaga aaatactggg cttgcgatac gcctccgatc gccgcctcct ccatcagttg
     4321 agctgcttcc tgtgtttggc tcgacagcat caggagcagc gccagacggt agcgcgcatc
     4381 tgggaaatgc ggttgcaagg cgagcgcctg tcgataggat tggattgctg tcttccgctc
     4441 gcccagtgag tagtacacgc ttcccagact gtaatgggca tccgtcaatg tgggatcgag
     4501 ccgcacagcc tcggtgagaa ccgaagcggc tgcccgccaa tcttgcttgg ccatgaacag
     4561 aatgcccaat tgtgagtgcg ctgtcgcgct gttgggattc aatttgacgg caagccggca
     4621 ttctgcaatt gcggcatcca gatcccccgt atcgtagagc gcttccgcca gggccatgcg
     4681 ctcgcctgca tgccttggtg acagtcgggt gagaccccgc aattcagcca gacgtttagc
     4741 ggcatgacct ggttcgaccg acgtgtgagg agatcgagcc tgttcatcac cgacgacttc
     4801 cggaggcggg ggtggacttt cgagaggagc aagcagagtt ggatcgtcag gcccttgagc
     4861 aaacgacgaa gcaatggcgc atgcgctgaa aatggccagg gccgataatc gacggcgcat
     4921 cgaccgagac tcctccgttc atcgaatggg caacattata ccaccgaggg ggaagcggtt
     4981 ggccgataag gcccggggtg aggagtcgat cacagctgct tcttgatcag cgaaatggtc
     5041 gtgcctacct gttccgggga gagggcgatg cggtcatgtg gaacgcgcca gaccggttcg
     5101 agaggagccg gatcgtgggc taggcgcggg ataaggtgcc aatgaatgtg aggccgttga
     5161 ttgcccagca actcgtagtt catttttctt gcctggaacg tttcggcaag caccttggtc
     5221 aagcggctga cttcttccat cagttggatg cgctcggtcg gtgcaagatg gaagagttcc
     5281 gtcgcatgac gcttgaatag gagcacggtc cagccgggaa agaactgatc gtcgtgcaga
     5341 taggccaagg acagacccag gtcggcaatg aagtgatcgg caagaggcca cacccctgca
     5401 caggcatcac acggcggatc gctcatggct ggatcgtttt ccgctcttct tcggtgatga
     5461 ggcctttttt gagcagcagt tggatcagtc tgtcagtctg ccgatcctgt ggcgaagggg
     5521 ctggctgagc ctgactgacc gacggcttgg gttccgcggg tggtcgtttt tttccgtaca
     5581 cgcggaatga cggggtgaag accgacacgt aatccctgtt gcgaatgcga atggaaagcg
     5641 gaaggctgtc ggtcagcgga gccagatcag ggctggcgcc caccggcacg cggaaaaatg
     5701 gtttgccgtc ttccgtttcg ccgtcctgcc ccacaatttc aaaccgattg aggagcggtt
     5761 cacttttcag cgcttcccgg tcttcgccgg cgagattggt gattttctcc agaacgatga
     5821 tgagtgaatc ggcgatcaac ggatcgctgg accggttccg gacggtcaga tcgtaccggt
     5881 actcgctgtt gaagttgtcg cgaccggtca aggtcacgct gacggacgcc ttgccggtga
     5941 gatcagccag ttgttcgcta ggcgaggcgt ctgccggacc tatgttgaat gcccagacga
     6001 tcagcagcat gccgatcagc agagtcgtgg ccaggtgaga tatccgcatg aatggtcgct
     6061 cgattcaaga tgacgattgc acgcgaagca tagcaaacat ctcaccattg atcgagtgtt
     6121 ctcgcgatct tgccttgaca ctcagtttct tcgacggata ttctgcgcgc gacgaaacgc
     6181 gagtcgaaga gccgatggac acccaacgcg ccccgctcct ggtttcccgc tctctcgttt
     6241 cccggctcat gggcctgtac gacgtgtctc atccccttca caagcgacgt gtcattcggg
     6301 gctatgaccg ggcccatgct gtccgcactg cgcgcatgtg cgctgccgtg gcctccgcat
     6361 tgggacatgg caccgagcgt gtgcgtcagt atcaaattgc ctgtctttta catgacttgg
     6421 gacgggccgg cctggatcgc cgcctgttcg gaaaaatctg gtcctgggcg aaggctcacg
     6481 ggattccaac gagaccccgc gagtggcgag ccatccatcc cgagacgccc tatggccgcg
     6541 agaccgaagc gttcgtcgcc cggtatagag aagcgctcga agctgacggc attcccgtca
     6601 ccgcctgggt aaaggaacag atagaaatgc ggctcgggta tgcccgccgt ttggcaaaga
     6661 gattacgggc ggtgagatcg tcactcacgc aaatgggggt gcgatttgaa ccctggatgc
     6721 agcgggtcat gctgtattac tactatccgg aaaagttgag ccgggcgcag ccctgggtga
     6781 aacagctggc ggaggtgctg gtcgcctgcg agcagttcga agcgtacagc aaccagcgtc
     6841 gcggccgcga ctattacgtg cgtagcaagg aatccatcgc ggaggccttc gcgtacctgg
     6901 ataaattgga gcaggagcgg attctcagcg tagaggtgat gaaggtgatg aaacggctgg
     6961 ccgccgccgg ggagttcaac ggggtgattg cggcagcacg aggccgccgg ttgacgcccc
     7021 aagagcgacg atttcttcgt cgaatggagg ggtgagtatg gcggtccaga cgctgcctct
     7081 ccggatcgac atgcgcggcg gaacccagat cgagaatgtg acgaagttcg tccaggaagc
     7141 gctggctcat gccaagatgt ctgccggcat cgtgaccgtg ttcgttcagc atacaaccgc
     7201 gtcggtcatg gtcatcgagg acgagccagg gatcagatcg gacacgaaaa cgttttggga
     7261 ccggatcgcc ccggctgatc agtcctggca gcataacgag caaaatccgg gagaagacaa
     7321 cggccatagt catctgagag gccagttgca aggtccttcc gtcaccattc ccttttcgaa
     7381 cgggtcgctg ctattgggga catggcaaca gctcgtggtg gtagatttcg acacccgcgc
     7441 gcgcactcgg gatctcatca ttcaaatcct cggagagtag ccttgatggt ttctgtcatg
     7501 gcgggactgc tggccgtgat gctccaactg gctggcgaag ggcgtttgtg tgatgttgcg
     7561 gctgccgatt gggggaaacc gctcggagcg ctctatgatg gtgtcgaggt gcggccacgg
     7621 ccggttaccc cggcccccac agagctgggc cctgatgaac gggctacgat ggcggtcttc
     7681 gagcgagcga ccaagtcggt cgtgtttatc tcgaataccg ccatccagcg ggatttttgg
     7741 tccttcgacg ttctggaggt cccccaagga tccggctcag gcttcgtgtg gaataagcag
     7801 gggcatattg tcacaaattt tcatgtgatt tatggagcga atacgatcaa agtcaccttg
     7861 gcagaccgca gcgagcatca ggccaaactg gtcggcgccg atcctgatca cgatttagcc
     7921 gtgctccaga ttcaggccgc cgagagcctg ctggaaccat tagccatcgg cagctcacac
     7981 gatctccgcg tcggtcaaaa agttctggcc attggcaatc cgttcggtct tgatcacagc
     8041 ctgaccacag gagtcgtgag cgcgctgggt cggaccatca aatcgctgtc caaccggacc
     8101 atcgaagggg tggtgcagac tgatgcggcg attaatcccg gcaattcagg tggaccgctc
     8161 ctcgatagcg ccgggcgctt gatcggcgtg aatacgcaaa tcatcagccc cagcggagcc
     8221 tacgcgggag tgggttttgc cgtcccggtt gatactgtca atcgtatcgt tcccgagctg
     8281 atcaaacatg gcaagctcat tcgtccgggg ctcggcgttt cccttgtgcc agatgctatt
     8341 gtgaagcgat ggggcatcaa ggggttggtc attggcaggg tcacgcaggg cggtgcagcg
     8401 gatcgggcag gacttagggg tgcccgagaa acccccacag gacgaataga actcggcgac
     8461 atcgtggtcg ccgtggccgg caagccggtc acgaccatcg acgagctgat ggctgtgttg
     8521 gaagaccaca aggtcggcga gcaggtgacc gtggaaatct tgcgaggaaa ccgccgcgaa
     8581 aagatcaccg tgacgcttca ggccgtcaat tagcaggcgg caatctccga tctctctatg
     8641 gtaggatctc tctcttatgt ggaggaccat ccatgagtga tcgccgcgca cccgactctc
     8701 ctcaagagcc gaccagcacg cgggaggatc cgggcacaaa gaccggttct gatcccctcg
     8761 agctcattga acagtgtctc gcgctgttcc ccgagtcaga tccccggcag aagctactgt
     8821 ataagttgcg tcatgccgtg atgctctccc aagcaggcaa ccagcagcgg gaagcggagt
     8881 ttagaaaagt cagcgaagta gtcgccaagc ttacggcacc cgccaaccgg ataggcatgc
     8941 tgctgggttt gcccggcgaa gacctcgccc atatcctggt gggaggcgcc gaatattatg
     9001 cgaatgtaga tccgcgggtc gccagcggcg atctcaaaat cgggacacag attcttgtga
     9061 acgaagccta tgcggtgatc aaaacactcg gctacgaccg gaacggcccg atcttgaaac
     9121 tgacggaggc gatggccgac ggccggctgc gctttgaaca agagatggga cggcaatcgc
     9181 tgatcttgca gcgatcgagc gatttggccg gtgtggagct gaaggcgggc gacgaagtcc
     9241 ggatcgacag cagtcttcgc atcgccatcg agaagctggc ggaccgcaag gccgccaaac
     9301 atgtgctcga cgaggcgccg aacgtcacgt gggaccaaat cggcggacag caggaagcga
     9361 tcgcggcgat tcgaaaggcg atcgagtatc cgttgctgca tgcctccacc ttcgagcagt
     9421 tcaagttctc ccaacccaaa ggatttctcc tttacggccc gccagggtgc ggcaaaacac
     9481 tcatcggcca ggcggctgct gcgagcctgt ccaagctggt cagcgagtct gctccggaca
     9541 ttcacgggac gggcgacgcg cgcgctccga tcaccggcgg ggcgttcctt catgtaaagg
     9601 gccccgagat tctcaacatg tggcttggcg aatcggagcg catggtgcgc gatctgttcg
     9661 tgcaggcgag agccaagcgc ctcgaggggg cactcccatt catctttatc gacgaagccg
     9721 agtccatcct tgggacgagg cgggccatgc ggtcgttcaa catctccaat accttggtgc
     9781 cgatgttttg cagcgaaatg gacggcatcg agtccctccg tgatgtcgtc attattctgg
     9841 cctcgaaccg gccggatttg attgacccag ccgtgctgcg gccgggccgg atcgatcgca
     9901 agatcaaagt cgcgcggccc aatcgagaag cctccgctca gatcttgaag gtgtacctgc
     9961 accacgatct gccactcgat cctgcgttgc ttgcggagcg cggcggcgaa cagggaagag
    10021 cgatggcttc gctcgccggc gatgtgcttg aacggatctt caaaagaagt gatgagaatc
    10081 gattgctgtc cgtccggctc cgaaacggcc agcagaaagt gctctaccga ggggacctgg
    10141 tcagcggcgc cattttggcg tcaatcgttc agcgggccaa ggaaaaggcc atcgaccgca
    10201 cgattcaatc cgggcagcca gccgggctgc tggctcagga tctgtttgat gcagtcacgg
    10261 aggaatttcg agaaggggaa gtgttaccgc ctgatgatgc agcggaagaa tggttgaaac
    10321 tgctcgatca tcatccggaa caagtcgtcg gcgtctcgtc ttttcggcga ggccggcaga
    10381 cggaagaaca gctcgtcaac cagatcatct aggcgcagtc tgccgctcca tgtgcatcta
    10441 tgtggggaag tgtgattcgg ctatttggca ttgaaacgga gtatgggatt gcccgggacg
    10501 acctggacac catggatccc gtggtcgagt ccatggagtt ggtacgggcg catctcacgg
    10561 gcccattcga gcggcggtgg gattatgcca gcgaagatcc acacgaagat gcgcgggggt
    10621 tccgggtatc gggtttggcg caggacaagg aagaggccca gttctcgagc atcgatgcgc
    10681 atcgcccatt ttcgtttcac gagatgaaaa gcgacctcgt gctgccgaat ggcgcgcggt
    10741 tttataacga ccatacccac cccgaatact cgtcaccgga gtgccggacg ctgaaagacc
    10801 tggtggctca tgatcgggcc ggggagcgca tcgtgcagcg cgcggccgat cgccgcaatc
    10861 agcggttggg aggcacgccg gtacagctct ataaaaacaa cacggatttc cacggacata
    10921 gctacgggtg tcatgacaac tatctggtat ctcgttccat tccgtttccg gcgctggtgg
    10981 cagggctgct gcccttcctc gttagccggc aggtcgtcgc gggcgccgga aagatgggta
    11041 ttgaagcaga atccggcttt gttcctggaa cctaccagct ttctcagcgc gctgatttca
    11101 tcgaagccga attgagcgtc gacacgatgc ataatcgccc gattctgaac acgcgggatg
    11161 agccgcatgc cgatgcgaag aagtatcgcc gcctgcacct cattatcggc gacgccaaca
    11221 tgtgcgaata tgccacggcg ctgaaagtcg gcaccacgca gctggtattg gagttgattg
    11281 caagaggccg cgcacctcgt ctggagttgg aagaaccggt cacggccgtg aagcagattg
    11341 ctcgggaccc cgatctcaag gccgctgtcc gattgaaaga tcgccggaac ctttccggat
    11401 tggagcttca agagtgttac tgggagacgg ctcaacggga gttggcgggc gccgatccgg
    11461 agaccgattg gattctctgt gaatggggcg agaccttgcg cttgctgatt cagcatcgcg
    11521 agcaactcgt gggaaggctc gattgggtca cgaaacagtg gctgctcgag acattcatcc
    11581 gcgaagagca gatcggctgg gacgacccct ggctggccag cctggatctg gaataccata
    11641 atgttaatcc cgagagaggg ctctatcgcg gtcttgaggc agagggaaag gtctggcgca
    11701 tgacgagcga cgcggacgtg gaggaggcct tggcgcgggg tcctctcgat acgcgcggcg
    11761 gtcttcgggg aatctgtgtc cggcgttttg cagatcagat caccgacatg caatgggagc
    11821 gaatccgctt ttccggcggc tggcggccga agatactcga catgggggac ctgttcgatc
    11881 ccctggaagt ccgtcgatgc gtcgcgttgt tcgaggaagc ggcttccccg gccgacgctc
    11941 ttgaggcatg gaagacacga aaggatcgca cattatgacc tacgacatgt taccggaacg
    12001 acgggaaggg ccgatcaatc ccatgccgaa atcgcctgat ccatcggagg agggtggagg
    12061 gccgcggcgg ccggaaagcg ggtctcccga caaagacaac ttgatgaaac gaatgaaaaa
    12121 agtcgatccc aaacaggccg aacgctaccg gcaacgaacg ggccagtaat attcgcgcag
    12181 ccggaatttt tccgggatcc ggacgccaca gccgacattt aactcagcgt aggtgacgaa
    12241 tgggtatgca gggagatttt tttcagctgt tgaaagcgca aggctatcaa ttcggtgcgg
    12301 caggccaggc cggcaacggc caggagttca cgactgcgac gaccattctg gcgttcaaat
    12361 atcgcgacgg tgtccttgtc gccggggatc ggcgtgcaac cgccgggaac atggtgatgt
    12421 acgatcgcac cgacaaagtc cttgaaatcg atcgtcacag cgtgatggcc atcgccggag
    12481 tgccggccac ggcctatgaa atggtacgaa tcctagaaca ttctttcaaa tactaccggc
    12541 gcacgcaatt gcaggaattg agctttgaag gcaagctccg tgcggtgtcg aagctcttaa
    12601 aggacaatgt ggctgcggcg ctggctggga cgggtgcggt cgctccgata ttcgccggct
    12661 atgactttga gcaggaaagc gcaaagattt tcttctacga cattctcggg gcggaattcg
    12721 agggtgtcga gtatgccgtc tcagggtcgg gctcgccgac catcagaggc attctgcatt
    12781 atctgaatac gtggggcgac cagccgcttc acgccatgag tgaggatcaa tcgaccattc
    12841 aagccctgcg gctgctgacg agtgcggcgg agttcgacag tgcgacaggt ggtgtgaatc
    12901 gcgaagcgaa tctttacccc gtcatcaagc tgatcactcg agatggtgtg cgcactctgc
    12961 cggaagccca gctgaagcaa agcttcgaag ccagcgtatc ccgattcgtc taggagtctg
    13021 tgactcggca gttatctttg tcgatcggag agcggaccga tgtatgaaga gccctataag
    13081 tgggtagaag cggttggcaa tcggcgccac tacctcgatg aacaatttac ccaaggcagt
    13141 cccgtcgtgg cgctggcgta tgacgcgggc attctcatgt tgacggtgag caagggcacg
    13201 ccaaaactgt atgaaatcta tgaccgactg gcattgggag gcatgggaca tccggcagat
    13261 ttggaaaagc tcagatttag cctcttggaa atggcgcatg tcgagggatt caaccgctca
    13321 ccgtcggatg tcacgggcag ccgcatggtg aagtatggca tcgcgccggt aatcaaacag
    13381 gccttcgagg aggtcttcaa ggctccgttc atcgtgaaga tcctgttggc ggaactcggg
    13441 cagaaaccgg gtaaagacat ctttctggtc atcaactacg atggcacgtt tgaggaaggt
    13501 ccacactgcg cggttctggc tgcgtcgaaa tcggttgagc aagacatgct ggcgtatctc
    13561 cggaagtccg ggacgatgca actcccgctg gaccaggcgg tcggagctgc acttcaagct
    13621 tgggccctcg gtgatcgatc gcggcgcaat gccctggcag cggagaacgt ggagggggcc
    13681 gcagccgggt ctccgccccc cgatgcggcc ggactctatg cctatgtccg tgaagcggtg
    13741 gccgagaaaa cacttgaagg cgccgtgcta gatcgtactc agccaggatc ttcgaagtat
    13801 cgggcactga cggcagaaga actcggccgg ctgctgccgc aggacatcaa aaagagctcg
    13861 ctgtaacgcc atgctcaaac gcatttttgg gttggagacg gagtatggac ttctggtcca
    13921 tcaagaccgg cccgaccact caccgacctg gttcgcgcac caaatccgtg atcatgtctt
    13981 ccacgttcag cggcagggcg tgctggatct gcatcatcgc gggcacgatg agccgccggg
    14041 gaacggcggt ttcttgacga atgccggacg gatttatctc gacatggggc atttggagta
    14101 tgcctcgccg gaatgccatt cgctgaccga cctcgtggcc agcgatcgag ccggagatac
    14161 cattttgcag cgggccgtcc acgatctcgg gctgggtgat caggtttcgt tgatcagaaa
    14221 caacgtcgac catgagacgg acgccacatt tggctctcac gaaaactact tggtcactcg
    14281 ccgattcccg ttcagccggc gtggtctcgc gccgctcgta acgtttctgg tgaccaggca
    14341 gattttcacc ggttcaggcc gtgtcggggc ggcaagcccc caggatgcct ggatacagat
    14401 ggatcggttg atcgtgccgc gggcggcgct aggccatcgg tcccaatccg cccagctccc
    14461 ttttcaaatt tcacaacggg cggaccacat cgtcaacgat ttctttgaat gggtgcagca
    14521 gaaccgcgcg atcgtaaaca cccgggacga gcccttggcc gacccgaacc agtaccgccg
    14581 catccacctc ttgctgggcg attcgaacat ggccgagtat gcgacagcct tgaaactcgg
    14641 aacgaccgga ttggtgttgc aactgatcga ggaaggcaag ggcccccgcg atcttgatct
    14701 cgacgaaccc gttgaggcgt tgcaggaaat ctctcaggac caggatcgcg aatggatcct
    14761 acggctgcag tccggcaagc gcatttccgc catcgacatt caggagcagt tcctggctgc
    14821 cgcaaagatg cattgcacag ggcaggacga tgaaacagac tgggtactcg aacaatggga
    14881 agccgtcttg caagatctcc ggggcgatta cggctgcctt gtgggacgtg tcgactgggc
    14941 gtcgaaattg tggttgttgg agaatttccg ggaggcggaa aagctggagt ggagcgatcc
    15001 aatgctgaag agcttggatc tggagtacca taatctcaac tctgagaaag ggctgtacta
    15061 tggattgctg caagaagggc gagtgccgcg cctgacgacg gacaaggccg tggaactggc
    15121 gatggagcat cctccccgca acacccgcgc gttcggtcgg ggcgaactgg tcaagcactt
    15181 gttgacctgt gggcccccgg cagaaccgga agctgcacgg aaagaagagc ggttgttccc
    15241 gtcttatgtc atcaattggt cgatcttcca gttacgcggg ctggagccct ttcccatgcc
    15301 ggatcctttc aagagttacg cgcaggaagt ccgctctcat ctcgtcaggc cgtaagcccg
    15361 ccagttaact gccctgattc aagcttaaag tcttgattca ccgtgggaaa atcgtccggt
    15421 gctatgctta tagctggttt aaccgcgtgg gaactacgtg gaggaggttc agcatgaaag
    15481 caaagcgatg gatgatggaa tatggagtgg ccatgatctt ggcgatacta ttggctgtcg
    15541 tgttagggca tttacctctc tttcgggaca ccgcggtggg aaagctgcac gcgtctgatg
    15601 tggttcaatt catgggttac ggcaccggca tcatattggc ctggtttgga gcacggcaac
    15661 ttgcggctga tccgcccgaa gagtggaagt ggctggtcgc ctatcgatca ctgattttgc
    15721 cggtgacgac tctcatcctc gtcgggctcg cctatcatgt cctgttgtat gtctctcagc
    15781 cctttctcgg taagtcgggc aaggagacgt ataactggac atttatcatc gggctcatcg
    15841 tctgctgcgg ctggttgatc ttgacctggc tccaaaagtg cgcgccacag atcgcatcgg
    15901 ccgaaccgcg gcggctgaga aaagctgcat agccattgcg tatccggctg cttcatacgg
    15961 aagcagccgg ttgtcctatc cccgtcctct tacaatctcg acgattcggt tttccaccca
    16021 gtaccggtct tcttcacttc gtctcgtctg aaaaaatccg caatggccgc catgagtagg
    16081 agcgagcaaa cggatggatg gattttgctg tacggaggga gtcaggaaca tggagtaggg
    16141 aataaaggga tcatcttgcg cggtgatgat cgtcgtgggg accgcaatgc tgtggaggac
    16201 gtgacgagca cctgaacgat cgtagtagtc tgccgcattg cgatacccgc cgtctggtgc
    16261 cgtgtaacgg tcgtcgaatt cactgatgcg cgtaatggcc cgcaagccct tcagatccca
    16321 cctgcccggc gacagcgacg ccttccgctg tacgcgcgcc ttaagccgtg tcaggaagtg
    16381 acgatgatag agccagttcg ccggccgttc caattcgcgg acacacaggg ttggatcgat
    16441 attcggacac accacgacgg tgcccgcaag ggccggttgg ctacggccca gttcaccggt
    16501 ggccttgagg agcaaattac cgcccatgga atatccgacg agccagatgc ggtgcatctg
    16561 ctcagcggct aattcctgca gcacgctccg gtaatctgca ctgaggccgc tgttgtacaa
    16621 agtgggagac agatgttccg tgcctccaca ggtacgttga tttaagcgca ccacattcag
    16681 ccctgccttg taggctttcg ccgcaatgcc atgcatataa tgtgaatcgc aacaaccttc
    16741 aagcccatgc aagaggagca tcgtttgtgc tgaccaccga ttgtcctgcc agtggcagaa
    16801 accgacgatc tgcgtatccg gctcggtccg aaagagccgc cgctcaaccg ggatgccggc
    16861 caacagcggt cctctcgacc agtagcgagg cacaacggtc atgagatgat gattccgaag
    16921 cgggcgagcg ggaacgaacg ccggcaattc catcgctgcc gcatcgcgag cgtgattaac
    16981 cgttctttgg cacttccctt cggtcacagt cctccttcaa gacgttgagg ccttcgctcc
    17041 cggcatgacc ggtcgataga gccgactcca ggctattccc atggaatcga gcaacggcgt
    17101 catgatggta gtcagcgcga tcaccatgat cccgttgcca aactgggcgg gagtgatgac
    17161 gttggaagcg agtagggttc ccaggatgac gaagccgaat tctccggcct gtgcaagaag
    17221 aaaggctaca cgcgcagcgg gtccatgttc catccggatg atccgggccg cgaggtacag
    17281 gacggcaacc ttcacgacta cgatcaccag aacatgcatg aggattctca cgggatgttc
    17341 ggttaagacg ctcacatcga ctgtaaggcc cacggcgacg aagaaaagtc ccagcaacaa
    17401 attcttgaaa gggatgactt catttctgag ttgatgccgc tgttctgtgc cggacaaggc
    17461 gactcccatc aggaacgcgc ctagcgccat cgacagaccc atgcgagagg cgacccaggc
    17521 gctgcccaat acggcgatga acaggaccgc cgtgaaggtt tcactcatgt gtttctcgct
    17581 tgccaggctg aacagccacg gacagatttt ccgcccaacg aagaaaagca gggccactgc
    17641 gccgacggcc ggcaatacct gccacaatga atggacgtcc gtcgatcccg ttcctgccag
    17701 cagaggcaca agggcgatga tgggcacaac ggtcaaatcc tgaaacaaaa gaacggcaaa
    17761 cgaggcttgg ccatgctcct ggtcgagctc gtttttcctt tccagaattt ggatgaccag
    17821 cgccgttgac gagttggcca acacgagtcc gagcaacagc cctaaggacc agctctggct
    17881 gaaccatcgc caataaagca tcaacaggat gcctgtcacc gccacttgca gcacgcccat
    17941 gcccagagcg taccgccgta acgaccaggc ttgtctgagg tccagctcaa ggccgatgac
    18001 aaacaaaaaa aacaccacgc ccaattctga gagctcccgc aagcgtgcca cgtgggcggg
    18061 ggcgagaaga tgcgggagtc cgagcaccac tcccgacagc aacaagccga gctcaggccc
    18121 aatacccacg cgccgtgcaa gaggaatcat gacggcgctg atcgtcaaca ggagggtgat
    18181 ggccaacagg atgtccggga ggtgcatggg aggcgggttc aacgtgtctc ctcctcgcgt
    18241 tctgctgcat tcatcactcc ttttcgagat gaggagagca tattcgtatt cccgtacgga
    18301 ccagcggacg aaaagtgtct gacttcaccc gatgtcacac cgtctggcga tgagatgcga
    18361 cggggtctat ggcaaagttt cgatgatgaa cgaaggtctg actgatccag gaaaagacac
    18421 acacaagaca tgaccatttt ctcgcaggag caagtttgga gcgatcatgt actagactgg
    18481 ctccccgggc aggactcgaa cctgcgacca gccggttaac agccggctgc tctaccaact
    18541 gagctaccgg ggaacgaggt tcaatttgaa gagggagtca ttctaacaaa ctactcgaag
    18601 aagggggaga ccttttttag acgtcgaaat cttctgtttc gtcctcgtca gaagaggcat
    18661 ctgtgttgcc ctgcgccagg gcagcataat gcttagccca cgtgagatag agattgccgc
    18721 tgtcccgcat tgtgtccgct gccttgacca gtttctcaac gagctctgga tccttgccgg
    18781 tcgcttggat aacggcggca cggcgctcga tggccttggg gtactccaag ataagccggg
    18841 agatctcctt cagcaattca tcaataatat tatcttcatc gcccatactg cacctcatcc
    18901 accaacaaaa aaccccatag cgcgaacgct atggggttgt gcaataccgt gtgagatcgg
    18961 ttacccctgg taggcttgtc cgagggctgc cgattctccc tttttcgaca tcaactgccc
    19021 ggtcgcactc acggcctgca tcaaaatagc ttcgtgcttg gcagcgttac agactaccac
    19081 gcacaactca caccccttgc agcgatcgat gttgacttcc gccaccatct tggccgagtt
    19141 gagatcgagg cagttcgcct cgggacaata catgatgcag aggcgacagc ctttcttggc
    19201 tgtacatttc tcgtcgatga cttgcgcgac gttatacatt acagttcgtt ccctttctta
    19261 cgcggcaacg gccggattgc cgacctgcaa ctcaaccttg ttcttttcgg cccattcgct
    19321 tgcgatctcg taggcacgct tcacggtcgc aagattctta gcgagcagca tttctttctt
    19381 cgcgaatttc ttcttgatcg cttcgtcgag agaggcagtg ccgcctgacg cgacaaactt
    19441 cttcccgaac cgctcctgaa gggcgccatc gagggcgtcc atcgtgacgc atttggtgat
    19501 gcctgatacc gatccgatca tggccatgtt ggtcgataac tcagtgccgg caacttcaat
    19561 ggcaagctcg gtgcccgcaa tgtaaaacac ggcaacattc aaatccttca gccgttggac
    19621 gtcctcttct gagagcagag gttgagcgga attaatgacg actacaccgc cctccttgac
    19681 gccggaataa aacggcatcg tatagctctt ccccatggta atgacctggg ggtggaacac
    19741 ctggatcacg tccggaaaca ccagctcgcc gcggtcgtaa atccgttcaa tgccaatgcg
    19801 gcagtagctc tcggccggcg ccatccgttt ttccgccccg aagaacggat tggaaataga
    19861 aaattttccg tcccggttgg cggccatagc caatacatgc gccgcggtga cggcgccttg
    19921 cccgcccaag cctgacatgc ggatattgag tcgcttcttg atcatgatgg cctccggttc
    19981 cgttaggctt ggccagccaa ctgcttggcg gccgctttcc gctccttgtc cttcgcggca
    20041 agttcggcca acaattgttt cgctggctcg ctgacgtatt ccttgtacgc gaaccgctcg
    20101 gctggcttct ccgaatcccg catttcctgc agcccttcca tgctgttctt gccgatttcg
    20161 agaatgcacg gcgtatagag ctgaagatag gtggggccga tttcccgcgc aatatggacg
    20221 gcgttgcgga tgaccttctc gaccagtgac ggcttgctga ccgtgcaatt gacgacataa
    20281 tgacaaccgg actcgcgggc gatctccggc agccggactt tatcgaacag cttgccgacg
    20341 ggcgccattt tggcgacgaa gcccttctgc atgagtccgc tctcctggcc gcctgtgttg
    20401 gcatagagtt cgttgtcgaa gcagatcgtc gtgaacttct cctggcggaa ccaggcttga
    20461 agggtcatgt ctagaccaat atcgaccgtg gcgccgtcac cggccaggac gaccacgtcc
    20521 ttgacgcggc cggggaaccg gacgctcagg gctcgtttca agcccgaagc gatggcgttc
    20581 tgattgccga acagtgagtg gatgttgtga acggcgacca ttgggaacac caggctggtg
    20641 cagccggttg aaccgaccat cacggtgtct tcgggattcg gcagtgaggc caggatatac
    20701 cgaaaggcca tggactccgg acagccggca cacaacgaat gctgttcgat cagttctttc
    20761 gacgaaccga tatccttcca gccacggtct tccttaccgt aggtcgcgct tttcacaaga
    20821 tcttgataat ccgacggcat gatgtcgtac aggtcttcag aaatcttgat ccgttccttg
    20881 ctcatgaggc ctcctcagat tcgctcgcgg gagcgaacag ttcaaatttg tgctgctcag
    20941 cttccacgac cggccaggga gaagctcttc atcccgaggg tcgtcttgat ttccgacacg
    21001 atgatttccg gcggcatggt catgcctccg cacacatggg gcccagcgtg tacacgatgc
    21061 gggtttggaa tcgtcgcccg gatttctttc gccaaccaac cggtaacgtt gaattcgggc
    21121 acgaaaatgt gcttggcgtt cttggtggct tcacggatct cttcttccgg ccagggccgt
    21181 agcgttttga tcttgatcag accacaacgc acgccctcgt cctccagaag tcggatggct
    21241 tctcgtcctt gcgacaccgc cgtgccggag gccacgatca taatttcttg gtcgatgtcc
    21301 tcggtatcga tcaggccgtt gagccagtgg atcgaatgtt tgcgcgagcg ttcaatcgcc
    21361 gcccaaattt cctgctgcca ggaggcatgg gtcgcatagc tgatgtaatt gcttttcatg
    21421 acgaacggat cacgcatcat gcgaaccggc ggacattcca tgtccatgca gggcaccggc
    21481 gagcggtagg gatcataagg cggcaggcac atgtcggccg gcgtgagatt caccacgtcc
    21541 ttcgtatgcg tgacaaagaa gccatcgcaa cagagggcca gcggcagatg cacatccggt
    21601 tcttccgaca ccatgtagcc cttcagaatc caatcgaaga agtcctgagc tgtttctgcg
    21661 tgccagacca acatcccggt attgagtaga taggcgattt ccagcgtgtc aggttgaatc
    21721 gacagcggcg agttgatgcc gcggcatgtg acaatcattt gaattggcaa gcgggcaccg
    21781 gcccacatgg ggaagttctc catggcgcgc atcgtgcccg gacctgccgt ggtcgtgaac
    21841 acgcgagccc ctccgaacgc cgcaccggca cactgcgaca tgacggcaaa ttcgctctct
    21901 ccgcggaagt aatctccgat atagccttcg gcaaacaatt ctccgatgag tgcggcagct
    21961 tcgctttgcg gcgtgattgg atacgcaatc atgacgtcac agctggcccg ccgaagcgct
    22021 tccttgatga cctcgctgcc tgtaaagaac gacggcgtgc gcggagcttc gtgcatcatt
    22081 ttccaggtat cagtatatgt ctgtcctttt ttattttggg tcccgataac tgaatgggtg
    22141 tctgccatgg accagcctcc tttcactgcg cacggtgctt caaacggctt agttcgaagc
    22201 cccgcttttg gccgatgtct gccgtggttg aacagtacct tttaagcgtt tgactgtgtc
    22261 cgccagtttc atgatttttt cgacagatgc atcacggaat gagagcatcg catcttcgtg
    22321 cccggcttgt gcgcacatcg cgatggtgta acaggaagtg ccgccgcgga tgatcttgag
    22381 tgacagattg gcttggtgac aatgcacacc gacgaacatg cagcaatcga tcttgttgtg
    22441 ccaaatagtc aggttggggt ggttcggatt gatttcgacc tcaggattga tcttgggata
    22501 tttcggtcga tagtcaggca tcggaatcag catggctgtc tgacccggct gcacacactc
    22561 cttcagtgtg ttgtagagat ggcggatcgc cgtggctttt ttagcggcct tttcgttcca
    22621 agcccagagg acaagcggtc cggggaaaat cgtcggcacc ttggccatta agaattgacg
    22681 ggcggcttcc tcatacgctt tttcttccga gacaatgacg ccattgatgt gggcttcacc
    22741 cggattcggc aggaagattc ccatggaagc cgctgcaggc ggaagaaaat gttctggccc
    22801 agggagcacg cgatattgac tcattacggc ctacactccg cgtatggggt taacgtctaa
    22861 agatgccaat tttcgaggaa cctacccgtg acaatctaag cttttttaga ggatctctgc
    22921 aacctcgcag aaggcccaac gtggggaaat aatgggctac tggacaacaa tggcttaggc
    22981 cttttgacca gtccccgcgg gatcttgccc gcaggttacg tcctcgatat caacggcgga
    23041 tttcgcatta taggagcggc actcgcgcga agtcaaacag acttccgtag gggaatatcg
    23101 tgctaagtaa gccgttaagc taagaaaaca gttggaataa cggtgtctta tagctgggcc
    23161 gtctcatgga agctgattgc aggctttgag ggatccgagc acgcatgcac gctcaaaatc
    23221 gtctttggcg agccgccctt gtagccgcgc ttgatagagg tgtgcccgtg tcaggagagc
    23281 gcccacatgc gtaggatcga tcaatagcac ggtcgacagg tcgtcgagcg cttgctctga
    23341 acggttgagt tttagcagga cgtcgctgcg aagaagaaag gcttccttct ggtaggggcg
    23401 ccagctgtcc ggaaccggtg cgtcgagcag tttgtggagc gtcagaagcg actgctgcca
    23461 ttgtttggct ttggcctgtc ggcgagcctg agtggtatag aggccgatga ggcgctgcct
    23521 cgccagatca aaaaaagggt ctaacgccag ggcacgctca taggccagcg aagcctcatc
    23581 ggtgcgcttc agccaggttt ggacgtcacc ctttccgatc cacgcccaag gattcttgac
    23641 gtccagctcc accgcttgat cgaaatcaga tctcgctttc tcgatatgcc ccgcgtactc
    23701 gccagacttc tggtgttctg aataggcgat cgagatcagc cgcaggtagg cttggcctct
    23761 gacgatcaag ggctcgacag actggggatc cagtcgggcg gcatcggagg ccaactctat
    23821 cttcttttcc aaatcgggag tatgaaacgc gagctcgagt tgctgcttgg cccgttgaac
    23881 cggttcaggc ttcccgctcg catcaatgct cagtacgcgc acgcccgacg gttgttgccg
    23941 caagtcttcg agttgcgcgc gcagacgccg gttctccaac tgcagttcac gaaaatgcct
    24001 tgccaactgt gcttctgact ggaggcgacg aatggcttcc gctaaattgt gtaaatccac
    24061 gctggcacga atgcgtatgg aaaagacagg acgctcgttc tcgaatgagc ggcgcgaatc
    24121 gagaatctcc gtttcggtga tggctgctgc gatcgtgcgg atctccaatg aggtgtttcg
    24181 agtggtgcgc ccgtatgatt cctgctcgac gtcgtagaac gtggattcca gatagacgcc
    24241 ggcctcttct accgctctcc gctgagcgcg ttgtagaacc ttttcttctg cggtcgccag
    24301 tgtgtcgcca tcagccatca cgtattgggc ttccgcgatg actgttcttg tgagggcaag
    24361 agcaggatga ccgctgaaga gtgcgaacgc cgaaatggca agagccagca caccccataa
    24421 tctcatagat ctaactatac cgctggaccc gtggctcggc aacgcgacgg tcttgggcgc
    24481 gggaatatcg taactcgctg aattactggg tcggtaacgt ggattttggg aagatctgtg
    24541 tgaaaggatg gacttcgacc cgttcgaata cgccgtggac cgtgtacgga tcttcgcgtg
    24601 caaaccgctc tgcctcttcc aaagaatcgg cctcaatgac gataaggctg ccggtcttat
    24661 ccgtaagagg gccggcgagc acgactcgtc ctgcttggtc gagcggttcc atgttggcca
    24721 ggtgagccgg acggtagatc tttcgtttgg cttctccctc gggaccatca aaaccaataa
    24781 tgacgaattt cattgctgac aggccatcac gatcaaaccg aatcttccaa tgggcatcgg
    24841 tctttgtatc cgctggtcgt ttcatgccac cattttattt cttgctcacc gagcttccag
    24901 cagagaaaca cgacccggcc ttccctgagg tgggggaagt cgcagagtcc gagatcgagg
    24961 tccttgacca gcactcccat ctcctggatg gcttgaacat tcgagctgat ttgatgcagg
    25021 ctggacaggt agagtggacc gaccgtgctt ccaccaccat attctgcccg tgcgctggca
    25081 cgacggatgt cgtccttggt gcgtgcgagg accgctttgg cctgacggac ggatgcaagg
    25141 cgggactgta actgcgggat taggtggttg gcttcggaca gcgtgaagag ccggtcgtgt
    25201 tcttgatcct cgtctccagc catcgtcaac tcctcctttg tgggtgtaac ataccattca
    25261 aaagtcctgc aaccgctgcc cgaagcagag ggtaaaggaa tcccgtaggc ctatcatcac
    25321 cgggccgctc gaggaaaaag aagtcgatgt tgacaacgat cgtcttgcag ggtatgctcc
    25381 acgatagtgt ttcatatcga gtccgcgtct tctccacaca cacaagactt ccgaggcatc
    25441 tctcgaaccg gatgattagc ttagggatga gttcggcttt gcaggcgcat ccacgatgat
    25501 catagcggac acgtttttca cggcacatcc cctctagcaa acacacaatt agccaactca
    25561 cgatcaaacc gtcattgcgc atcgttttcc atggttgatc atattccgcg gcgacacttg
    25621 tctttgacca ttacccccga gcttgaggat atgtatggca caaactaagg gagaagttat
    25681 gcatcttgcc gacttgaagc agaaaaccat cgccgatctg aacgacgtcg cccgcgacct
    25741 gaaaatcgaa ggggccgcga atcttcgaaa gcaggaattg atcttcgcca tcctccaggc
    25801 gcagacggaa aagaacgggg tggtgttcgg agagggcgtc ctcgagactc tgccggacgg
    25861 gtttggtttc ttgcgcgcac ccgattccaa ttacctgccc ggtcctgacg acatttatat
    25921 ctctccttcg cagatccgcc ggtttaatct cagaaccggc gatattgtct ccgggcagat
    25981 cagacctccc aaggaaagcg aacgctattt tgcgctgctc aaggtcgaga aggtgaactt
    26041 cgaagaccct gaggtggctc gagacaaaat cctcttcgat aacctgaccc ccctctatcc
    26101 cgaagagcgc attcaactgg agttcgatag agaagaatat tgcacgcgtg tcatggatct
    26161 gacgaccccg attggaaagg gccagcgcgg actgatcgtc gcggcgcctc gcacggggaa
    26221 aacgatgttg ctccaagcca tcgcgcgggc catcctgaag aatcacaaag aagtgacctt
    26281 gatcgttctc ctgatcgatg aacgtcccga agaggtcacc gactggcaac ggcaggtcaa
    26341 agccgaagtc atcagttcta cgttcgacga gccggcccaa cgacatgctc aagtggccga
    26401 aatggttctc gagaaggcta aacgattggt cgaacacaag aaggacgtcg tcatcctgtt
    26461 ggacagcatc acccggctgg cgcgtgccta caacacgatt gctcctccaa gcgggaaggt
    26521 gctctcaggc ggtctcgact caaacgcctt gcaacgtccg aagcggttct ttggggctgc
    26581 gcgtaatatt gaaaatggcg gcagtctcac gatcatggcg acggcgctgg tggataccgg
    26641 cagtcgcatg gacgatgtga tctttgaaga gtttaaaggg accggcaata tggaggtcca
    26701 tctcgatcgc cggttggccg acaagcgtat tttcccggct atcgatatca gtcaatccgg
    26761 cacccggaaa gaagagttgc tggtggacaa ggaccgtctc aataagatgt ggattttacg
    26821 caaggtcctg agccctctgg ggacgatgga agccatggaa ttcctcatgg ataaggtgca
    26881 cggcacgaag accaaccagg aattcttgca atccatgaac cgctgaacgc ttgtatctct
    26941 cggtcggcac aggttaaagt gacgcgcctt cgaataagcg gaggcgagag gccctgacgc
    27001 caggagtgga attatgaaga agggtattca tccattatat agagaggcga cagttcattg
    27061 cgcctgcggt aacagttata agaccagatc gaccgtcgga gacatcaacg tcgacatctg
    27121 ctcgagctgc catccatttt tcaccggcac gcaaaagatc gtcgacaccg aagggcgggt
    27181 cgagcggttc aaaaagaagt acgcgaagaa gggcaagtag tcgggttcac gaacatacga
    27241 gagaccctgc ttcgcagagg cagggtctct ttttttgtgt ccagatcgcg tgcaatcgag
    27301 gatggtgtat ggaagcgggg ctattgaaaa aatgggaatc ggccgtcaac cgacatgacg
    27361 agttgaccac tcagctcatg gatccgtccg tgatcagcca gccggcctt
//
LOCUS       DASSCQ010000058         7248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_366416, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000058 DASSCQ010000000
VERSION     DASSCQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366416"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFH05_04170"
     CDS             <1..880
                     /locus_tag="VFH05_04170"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET6677437.1"
                     /translation="AEIGDLRRAGCVAISDDGKPVMNSLVMRRAMEYALAFDIPVIDH
                     CEDLHLAEGGCMNEGLISTELGLPGIPAAAEDVMVARNVSLAELTGARLHLAHISTVG
                     SVRMVREAKSRGLRVTAEACPHHFMLTEEIVRGFNTLAKMNPPLRTWQDVQAIKDGLR
                     DGTIDVIATDHAPHATQEKQQGFTEAPFGIVGLETALPLTLALVEEGILSLESAVEKL
                     SAAPARAFSLAKGTLQPGADADVAIIDPQAQWEVDPGKFRSKSRNTPFAGWKVKGLVM
                     TTIVSGRVVFDAAGSA"
     gene            883..1398
                     /locus_tag="VFH05_04175"
     CDS             883..1398
                     /locus_tag="VFH05_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HET6677438.1"
                     /translation="MHCGWGLACALSVEYLALGIWVGGLIVLVGAVIPAVFNTLGGQE
                     SGGFFLTKAFEGYNRLVVVALIILVCAMGYRRWQGTARATVSRGEWVLLSVMGAIAGV
                     IILVLHPHAAALQAQAFALKDEQARKAAFEAFFRFHMPIRSLYVVNVVLGVWLLALKA
                     KRSIEPERIPA"
     gene            1395..2567
                     /gene="carA"
                     /locus_tag="VFH05_04180"
     CDS             1395..2567
                     /gene="carA"
                     /locus_tag="VFH05_04180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET6677439.1"
                     /translation="MKRALLALADGKVFEGRALGFEGETVGEVVFNTAMTGYQEVLTD
                     PSYKGQIITMTCPHIGNYGVTPEDAESSRIWAEGFVVKEASRGASNWRSRQGLHDYLK
                     AARIVGIEGLDTRALTRHLREHGSQQGLITHVEGNAERAVEKARQAPDIVGRDLAAEV
                     TCQNKYRWTDGSGEWVPASHGSATASLRRWKVVAYDFGVKHNILRRLVDVGCDVHVVP
                     ASTTAEQVEALAPDGVFLSNGPGDPEGVGYAIKTMRQLIGRRPIFGICLGHQILGLAL
                     GFKTYKLKFGHHGANHPVINLRTKQVEITSQNHNFAVQSPELPEGIPDRPPLLDSDFG
                     RVAVTHMSLNDRSIEGLACLDQPVFSVQYHPEASPGPHDSAYLFAEFVQLMEKSHG"
     gene            2560..3528
                     /gene="sppA"
                     /locus_tag="VFH05_04185"
     CDS             2560..3528
                     /gene="sppA"
                     /locus_tag="VFH05_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET6677440.1"
                     /translation="MDKIQRTVVGLFVLCSVFMQQGCVTVNLLEPPGPVQEVQLSGSG
                     DGKVLLLDLSGVISSQDKEGWLPQPNLLATFKEELTKASKDEKVKAVVVRINSPGGTV
                     NASDILYHELKTFKENKKVPVIASMMDVAASGGYYLAMATDAILVHPSTVTGSIGVIM
                     LTVNAKGLLEKVGVEANAITSGPRKDMGSPFRSMTADEKEIFQGVIDSFYQRFLTVVQ
                     AGRPQLSAEQIKKLADGRIYSGDQAKAAGLVDDIGYLDEAIDLAKKNAGLKEARVVTY
                     RRHGEYQNNIYSRVFGSGNGLSSLASMDLLSMVRGGSPQFMYLWMP"
     gene            3536..4519
                     /locus_tag="VFH05_04190"
     CDS             3536..4519
                     /locus_tag="VFH05_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6677441.1"
                     /translation="MALSAKDTYRSDSDSLPQVLATPVGRRAMLAWGARGALGLWTAL
                     GTGSALAVLSALSGCYRAPGTARDQLIFFSEEKELQFGLSAYREVLRQARLSENAEIN
                     EQIQRVGRKIADVANKPEYQWEFAVIEDDKMVNAFCLPGGKVAVFTGILKHTKTDAGL
                     ATVMGHEIAHALQRHGVERMSRSIIDQIAQLGALGAAAAGHSSGGAIQGLLGAYGVNV
                     SLPFNRKQESEADYIGLRLMSQAGYDPREAVPFWERMSGCPRQMIDKLCFRSQHAIPE
                     FLSTHPSDVTRINQLEAWLPEAMRYYHGAGGGPMPAPAPYKPAIGPLPQTS"
     gene            4526..>7248
                     /gene="carB"
                     /locus_tag="VFH05_04195"
     CDS             4526..>7248
                     /gene="carB"
                     /locus_tag="VFH05_04195"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET6677442.1"
                     /translation="MPKRTDIQSILLIGSGPIVIGQACEFDYSGTQACKALKDEGYRV
                     VLINSNPATIMTDPEFADRTYVEPITLDVIEKVIERERPDALLPTMGGQTALNATMGL
                     VKRGTLDKYGVALIGASAEAIHKAEDRDAFKQAMQRIGLRVPESGTAHSREEAIRVLD
                     RVGFPAIIRPSFTMGGTGGNIAYNREEFERLIEWALAMSPVSQVLIEQSLIGWKEYEL
                     EVMRDLKDNVVIVCPIENLDPMGVHTGDSITVAPAMTLTDKEYQRMRDAALRIIREIG
                     VDTGGSNIQFGVNPDTGELVVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     ITNDITGVTKASFEPTIDYVVVKIPRFAFQKFKGADPTLTTQMKSVGEAMAIGRTFKE
                     SLQKAIRSLELELSGLASRVGLDRGIPEGFDRQGAMERIRQQLRSPIAERIWYVADAI
                     RLGMSNDELFALTKIDAWFLDQLRDLMRVEQRLVEQASGQASLDDALLWEAKEQGFSD
                     ERIAQLTGLAAASIRAQRSAPGSTRSVTYKRVDTCAAEFEAHTPYLYSTYGTECEARP
                     TAREKVIILGGGPNRIGQGIEFDYCCVHAAMALREEGLETIMVNCNPETVSTDYDTSD
                     RLYFEPLTEEDVLNIIQRENPLGVVLQFGGQTPLKLAIPLSKAGVPILGTSPDAIDRA
                     EDRERFRDLLNRLGLQQAESGIARSVDEAVSIAANISYPVMVRPSYVLGGRAMQIVYD
                     QAGLLEYMGSAVKASHKHPVLIDKYLSDAIEVDADAICDGRRVVVAGIMEHIEEAGVH
                     SGDSACSLPPYSLDETVVEEIRRQMTALALELGVVGLMNAQFAVKDKTVYVLEVNPRG
                     SRTVPFVSKAIGVPLAKLAMKTMIGRSLDDLGFVTAPKPVHLSVKEAVFPF"
BASE COUNT         1555 a         1933 c         2305 g         1455 t
ORIGIN      
        1 ggccgagatc ggcgatctcc gccgggccgg ctgcgtggcc atctcggacg acgggaagcc
       61 ggtgatgaat agcctggtga tgcgaagggc gatggaatat gccctggcat ttgacatccc
      121 ggtgatcgat cattgcgaag atctgcatct ggccgaaggc ggatgcatga atgaagggct
      181 gatctctacc gaactgggcc ttcccggcat tccggcagcg gctgaggacg tcatggtggc
      241 acgcaacgtg tcgttggccg agttgaccgg cgctcgcttg catctggcgc atatcagtac
      301 cgtcggatcc gttcgcatgg tccgcgaagc aaaatcccgg ggacttcggg tgacggcgga
      361 ggcctgtccg caccatttca tgttgaccga agaaatcgtt cgcggcttca acacgctggc
      421 aaaaatgaat ccgccgctcc gaacatggca agacgtgcag gccatcaagg acggattgcg
      481 ggacggcacc atcgatgtga ttgcaacgga tcatgcgccc cacgcgacgc aggagaagca
      541 gcagggtttc accgaagcgc ctttcggcat cgtgggattg gaaacggccc tgccgctgac
      601 gctcgcactc gtcgaggaag gcattctctc gctcgagtcg gccgtcgaaa agttaagcgc
      661 ggcgccggcc agagcattta gcctggcgaa agggacgttg caaccgggag cggatgccga
      721 tgtcgccatc atcgaccccc aggcgcaatg ggaagtggac ccgggcaaat tccgctcgaa
      781 aagtcgtaat acgccgttcg cgggttggaa agtcaaagga ctcgtcatga cgaccattgt
      841 gagcggccgc gtcgtgtttg acgcagcggg gtctgcgtga ggatgcactg tggatggggt
      901 ctggcctgtg cgctaagcgt ggagtacctt gcgctaggta tctgggtggg aggattgatc
      961 gtgttggtcg gtgcggtgat tccggcggtc ttcaatacgc ttggcgggca ggagtcgggg
     1021 ggattctttc tcacgaaagc gttcgaaggc tacaaccgac tggtcgtcgt cgcgctaatt
     1081 attctggtct gcgcgatggg atatcggcgg tggcaaggca cggcacgcgc aacagtcagc
     1141 cggggagaat gggtgctctt gagtgtgatg ggcgccatag cgggggtgat tattctcgtg
     1201 ctgcatcctc acgcagcggc cttgcaagct caggcgtttg ccctcaagga tgaacaggca
     1261 agaaaggccg cgttcgaggc gttctttcgg tttcatatgc cgatccgctc gctgtatgtc
     1321 gtcaatgtcg tgttgggtgt gtggttgctg gcgctcaaag cgaaacggtc tatcgagcca
     1381 gaaaggatac ctgcgtgaaa cgggcgctgt tagcattggc ggacgggaag gtcttcgaag
     1441 gccgcgcgct cggatttgaa ggcgagacgg tcggcgaagt ggtgtttaac acagccatga
     1501 ccgggtacca ggaagttctg accgatccct cttataaagg acagatcatc accatgacct
     1561 gtccacacat cggcaattac ggggtgacgc cggaggatgc cgagtcgtca cgcatttggg
     1621 ccgaaggctt tgtcgtgaaa gaggcgagca ggggagcgag taattggcgc agccggcagg
     1681 ggcttcacga ctacttgaaa gcggcgagga ttgtggggat tgaggggctc gacacccgag
     1741 ccctgacgcg gcatttacgt gagcacggct cgcagcaggg actcatcact catgttgaag
     1801 gcaacgccga gcgggcggtg gaaaaagctc ggcaggcgcc cgatatcgtc ggaagggatt
     1861 tagccgccga ggtcacctgt cagaacaagt accggtggac cgatggttcc ggcgaatggg
     1921 ttccggcatc ccatggcagc gcgacggcct ctctacgccg gtggaaagtc gtcgcctatg
     1981 atttcggggt caagcacaac atcctgcgcc ggctggttga tgtcggctgc gacgtgcacg
     2041 tggtgcccgc ttcaacgacg gcggagcagg tagaggctct cgcgccggac ggtgtctttc
     2101 tgtcaaacgg tcccggcgat cccgaaggcg taggctatgc cattaaaacg atgcggcagt
     2161 tgatcggtcg tcggcctatt ttcggtattt gtctgggaca ccagatcctg gggttggcgt
     2221 tggggttcaa gacctacaag ttaaaattcg ggcatcatgg agccaatcat cctgtgatca
     2281 atctcagaac caaacaagtc gagatcacgt cgcagaacca caattttgcg gtgcagtctc
     2341 cggaactgcc ggaaggcata cccgatcgtc ctccgctcct cgatagtgac tttggtcggg
     2401 tggcggtgac acatatgagt ttaaacgacc ggtcaattga ggggttggcc tgcttggatc
     2461 agcccgtctt ttcggtacaa tatcatccgg aggcttctcc ggggccgcat gattcagcct
     2521 atctgtttgc cgaattcgtt cagttaatgg agaaatcgca tggataagat acagcgcacg
     2581 gtggttgggc ttttcgtcct gtgctccgtc ttcatgcaac aggggtgcgt cacggtcaat
     2641 ctgttggagc cacccggccc ggtgcaagag gtgcagctca gcgggtctgg tgacggcaaa
     2701 gtgttgctcc tggatttgtc aggcgtcatc agctcccaag ataaggaggg atggctgccg
     2761 cagccgaatc tcctggcgac gttcaaggaa gagctgacca aggcatcaaa ggacgagaag
     2821 gtcaaggcgg tcgtggtgag gatcaacagt cccggcggga ccgtcaatgc ctccgacatt
     2881 ctgtaccacg agctcaagac gtttaaggaa aacaaaaaag ttccggtcat tgcttccatg
     2941 atggatgtcg cggcgtccgg cggctactac ctggcgatgg caaccgacgc catcttggtg
     3001 cacccctcaa cggtcaccgg tagcattggc gtcatcatgt tgacagtgaa cgccaagggc
     3061 ctcttggaaa aggtcggcgt cgaggccaat gcgattacgt cggggcctcg caaggacatg
     3121 ggttctccgt tccgatcgat gaccgcagac gaaaaagaga ttttccaagg ggtgatcgat
     3181 tcgttctatc aacggttctt gacagtggtg caagcaggac gtccgcaact ctcggcggag
     3241 cagatcaaaa agctcgcgga tgggcgcatt tactccggtg atcaagcgaa agcggcaggg
     3301 ttggtcgatg acatcgggta cctcgatgaa gcgattgact tggcgaagaa aaatgccggt
     3361 ctgaaagagg cgcgtgtcgt cacgtaccgc cggcatggcg aatatcaaaa caacatttat
     3421 tcacgggtgt ttggaagcgg aaacggcctc agcagtctcg caagcatgga cctattgtcc
     3481 atggttcgcg gtggcagccc acagttcatg tacctgtgga tgccgtagga gtgacatggc
     3541 gttgtcggca aaggatacgt accgatcgga ttccgactcg ctgccacaag tcctggcgac
     3601 gccggttgga cgacgagcca tgctggcctg gggcgcacgc ggcgccctcg ggttgtggac
     3661 cgcgctggga acaggaagcg cgcttgcggt cctcagtgcg ctttccgggt gttatcgcgc
     3721 cccgggcacg gcccgcgatc agctgatttt cttttcagaa gaaaaggaac tgcagtttgg
     3781 gcttagcgcg taccgggaag tgttgcggca ggcacgcctg agtgaaaacg cggagatcaa
     3841 cgaacagatt cagcgggtcg gtcgaaaaat tgccgatgtg gcgaacaagc ccgagtatca
     3901 gtgggagttc gccgtcatcg aagacgacaa gatggtaaat gcgttctgtt taccaggggg
     3961 aaaagtcgcc gtgttcaccg gcattctgaa acacacgaag accgacgccg gattggcgac
     4021 cgtgatgggg catgaaatcg ctcatgccct gcagcgacac ggcgtggaac gaatgagccg
     4081 aagcatcatc gatcagatcg cccaactcgg cgccctcggt gcggcggccg caggtcattc
     4141 gagcggaggc gccattcaag gattgcttgg tgcctatggc gtgaatgtct cgctgccgtt
     4201 caaccgaaaa caagagtcgg aagccgatta catcggcctg cgattgatgt cgcaagccgg
     4261 atatgatcca cgcgaggcgg tccccttctg ggaacgcatg agcggttgcc cgcggcaaat
     4321 gatcgacaag ttgtgttttc gctcgcaaca tgcgatccct gagtttctct cgacccaccc
     4381 atccgatgtg acccgcatca atcaattgga ggcgtggtta cctgaagcga tgcggtatta
     4441 tcacggagcg ggaggagggc ctatgccggc tcctgcccct tacaaaccgg ccatcgggcc
     4501 gttgcctcag accagttaag tctgcatgcc gaaacgcact gatatccaaa gcatcctctt
     4561 aatcggatcg gggcccatcg tcatcgggca ggcctgcgaa ttcgattatt ccgggaccca
     4621 ggcctgcaag gcgcttaagg atgaaggcta ccgggtggtc ctcatcaaca gcaatccggc
     4681 gaccattatg acggatccgg aattcgcgga ccggacgtac gtcgaaccga ttacgctgga
     4741 tgtgatcgaa aaagtcattg aacgagaacg cccagatgcg ctgcttccga ccatgggagg
     4801 gcaaacggcg ctcaatgcga cgatggggtt ggtgaagcga ggaacgctgg ataagtatgg
     4861 cgtggccctg attggagctt cggcggaggc cattcacaag gccgaggatc gagatgcctt
     4921 caagcaggcg atgcaacgga tcgggctgcg cgtgccggag agcgggaccg cccatagccg
     4981 cgaggaagcc attcgtgtgc tcgaccgcgt gggatttccc gccatcatcc ggccgtcatt
     5041 tacgatgggc ggcaccggcg gcaacatcgc ctacaatcgg gaagaattcg aacgattaat
     5101 cgaatgggcc ttggccatga gtccggtgag ccaagtcttg atcgaacagt cgttgatcgg
     5161 ctggaaagaa tacgaactcg aggtcatgcg ggatctcaag gacaacgtcg tcattgtgtg
     5221 ccccatagaa aatttggatc cgatgggtgt gcataccggc gacagcatca cggtggcacc
     5281 ggccatgacg ctgacggaca aagaatacca gcgcatgcgt gatgccgcgc tccgcatcat
     5341 ccgggagatc ggggtcgata ccggcggttc gaacattcaa ttcggcgtca atcccgacac
     5401 cggggagctg gtcgtgattg aaatgaaccc gcgggtgtcc cggagttcgg ccttggcgtc
     5461 gaaagccacc ggctttccca ttgcgaagat tgcggcaaag ctcgcgatcg ggtacacact
     5521 cgacgagatc accaacgaca tcaccggcgt caccaaagcc tcgttcgagc cgacgatcga
     5581 ctacgtcgtc gtaaaaattc ctcgttttgc ttttcagaaa ttcaaaggcg ccgatccgac
     5641 attaaccacg cagatgaaat cggtcggcga ggcgatggcg atcggtcgga cgtttaaaga
     5701 gtcgctgcag aaagcgatcc gttcgcttga actggagctg agcggccttg cctccagggt
     5761 cggcttggac cgcggcattc ccgaagggtt cgatcgacaa ggcgccatgg aacggatccg
     5821 tcagcaactg cgaagtccca ttgccgaacg gatctggtat gtcgcggatg ccatccggtt
     5881 ggggatgtcg aacgatgagc tctttgcgct gactaaaatt gacgcgtggt tcctggatca
     5941 gttgcgggac ctgatgagag tcgagcagcg gctggttgaa caggccagcg ggcaggcatc
     6001 cttggacgac gcgctgctct gggaagccaa agaacaaggg ttttcggacg agcggatcgc
     6061 acagctgacg ggtctggcgg ccgcttcaat cagggcgcag cgatcggcac caggaagcac
     6121 gcgatcggtc acctacaagc gcgttgatac ctgtgctgcg gagttcgaag cgcatacccc
     6181 gtatctgtac tccacctatg gaacggagtg cgaggcccgt ccgacggctc gggagaaagt
     6241 gatcatcctc ggcggcggac ccaatcggat cggccagggg attgaattcg attactgttg
     6301 cgtgcacgcc gccatggcgc ttcgagagga aggactcgaa acgatcatgg tgaactgcaa
     6361 ccccgaaacg gtgagcaccg attatgatac gtccgatcgc ctgtatttcg agccgctgac
     6421 cgaagaagat gtgttgaaca tcattcagcg tgaaaacccg ctcggcgtcg tgctgcagtt
     6481 cggcgggcaa acgccgctca agctggccat cccgttgtcg aaagccggcg tcccgattct
     6541 cggcacgagt ccggacgcga ttgatcgtgc tgaagatcgt gaacgctttc gggacctgct
     6601 caatcggcta gggctgcaac aggccgaaag cggcatcgcc cggtccgtgg atgaggcggt
     6661 gtcgattgcc gcgaacatca gttacccggt aatggtcagg ccctcctacg tgctgggcgg
     6721 acgggccatg cagatcgtgt acgatcaagc cggcctgctc gagtacatgg gatcggccgt
     6781 caaagcctcg cacaaacatc ccgtgttgat cgacaaatac ctttctgatg ccatcgaggt
     6841 tgatgccgat gccatctgtg acggccggcg ggtggtcgta gcgggcatta tggaacatat
     6901 cgaggaggcc ggcgtgcatt ccggcgactc ggcctgttcg ctgccgccct attcactcga
     6961 tgaaaccgtg gtggaggaaa tacgacgcca gatgacggca ttggcattgg agctgggggt
     7021 ggtcggcctc atgaatgctc agtttgccgt gaaggataaa accgtctacg tgttggaagt
     7081 caacccgcgc ggctccagaa ccgtgccgtt tgtcagtaag gcgatcggtg tgccgcttgc
     7141 gaagctggcc atgaagacga tgatcggccg atcgttggac gatcttggat tcgtgacggc
     7201 gcccaagcct gtccacctct cggtgaaaga agccgtcttt cccttcaa
//
LOCUS       DASSCQ010000059         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1684661, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000059 DASSCQ010000000
VERSION     DASSCQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684661"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFH05_04200"
     CDS             complement(<1..265)
                     /locus_tag="VFH05_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6677443.1"
                     /translation="MIASLLEFSLRQRILILGVACVVSIAGVLAFQSLPIDAYPDVTN
                     IQVQVLTEAPGLSPVEVERFITYPLELQMTGLPGLAEIRSLSKF"
     gene            complement(262..1470)
                     /locus_tag="VFH05_04205"
     CDS             complement(262..1470)
                     /locus_tag="VFH05_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6677444.1"
                     /translation="MATNPLKGRTVKSIGGQTGFLLVAIVACATGCENTQQDSSSAMP
                     MTSSRPAGLVRLSQEELSRTTIEIIPVERGRFRLSREFPATIHPNENELAEVTTLIRG
                     RVVKVQVDVGADVKKGDLLALLHSTDLGLAEGAYLKASARLYEADLSYKRAKDLYEHR
                     AVSLAEFQRREAAMRTARAETREMENQLELLGVPRREIDRLDREHTIKADVVLRAPFD
                     GRVIMRNITRGEVLETNHTCFTVADLSDVWVIAAVPEKDVEYIQRGHTVDIVAAAYPH
                     GIFSGTITHVGDVLDPATRTLPVRVTARNPDKRLKPEMFALVRIRATPDPNTLTVPLS
                     SIQNGPTGHIVFVQRAPSEFEVRTVRLGEEYGDVAAVLDGVRAGEPVVTKGSFVLKSE
                     MERHKIEPAP"
     gene            1747..2232
                     /locus_tag="VFH05_04210"
     CDS             1747..2232
                     /locus_tag="VFH05_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET6677445.1"
                     /translation="MVILQRLQNYLKIHQIPYEEIPHSTAYTAREVAENLHVPAKMYA
                     KVVVVKADSRFVMAVLPSSWLVDLKRLEEVLGYSRVRLATEDELATLFPDCEVGTMPP
                     FGNLYGLPVYVDELLAKNEDMYFDAGTHKGAIKLRYQDFADRVHPQVAEFHREPTKLE
                     Y"
BASE COUNT          542 a          703 c          689 g          487 t
ORIGIN      
        1 cgaatttcga gagcgaccgg atctcggcga gaccaggcaa tccggtcatc tgaagctcga
       61 gcggataggt gatgaaccgt tccacctcga ccggtgacag cccaggcgct tccgtcagga
      121 cttgaacctg aatatttgtc acgtccggat acgcatcgat gggaagagat tgaaaggcaa
      181 ggacgccggc aatggacacg acgcacgcga caccgagaat gaggatccgc tgacggagag
      241 aaaattccag aagggaggcg atcatggcgc aggttcgatc ttgtgccgtt ccatttcgga
      301 cttgagcaca aacgagccct tggtgaccac cggctcacca gcccgaacgc catcgagtac
      361 agcggcgaca tcgccatatt cctcgccaag acgcacggtc ctcacctcga attcggaagg
      421 ggcgcgttgt acaaagacga tgtgacccgt cggtccattt tgaatggacg agagcggcac
      481 ggtgagtgtg ttcggatccg gcgtcgcccg aatgcgaacg agcgcaaaca tttcaggctt
      541 caagcgtttg tcgggattgc gagccgtgac cctgaccggc aaggtgcggg tggcaggatc
      601 caacacgtca ccgacgtggg taatggtgcc gctaaagatg ccatgaggat aggcggcggc
      661 cacgatgtcg acggtgtgac ctctttgaat gtactccaca tccttttcag gcacggctgc
      721 gatcacccac acgtcggaaa ggtcggcaac cgtaaaacac gtgtggttgg tctcgaggac
      781 ctctccgcgc gtgatgttgc gcatgatcac ccggccatca aacggtgcgc ggagcaccac
      841 gtcggccttg atcgtatgct cacgatccag ccggtcgatt tcgcggcggg gcacgccaag
      901 aagttcgagc tggttctcca tttcacgtgt ctccgcacgg gccgtcctca ttgccgcttc
      961 ccgtcgttgg aactcggcca aactcaccgc acggtgctca tacaaatcct tcgcccgctt
     1021 gtacgacaag tctgcttcat agagcctggc actggccttg aggtaggcac cttccgccaa
     1081 tcccaggtcg gtgctgtgca ggagggcaag gagatcgcct tttttcacgt cggccccgac
     1141 atcgacctgc actttcacga cacggccacg aatcaatgtc gtgacttccg cgagttcgtt
     1201 ttcattcggg tgaatggttg cgggaaactc gcgggacagc cgaaaccgtc cacgttcgac
     1261 gggtatgatc tcaatcgtgg tccgagaaag ctcttcttgg ctgaggcgca ccagtccggc
     1321 cggcctgctg gaggtcatcg gcatggccga cgaactgtct tgctgagtgt tctcacagcc
     1381 cgtcgcgcag gcaacgatgg ccacgagcag gaaacccgtc tgaccgccaa tcgacttgac
     1441 ggttcgcccc ttcaatggat tagtggccac aaagcactcc tagacacctg cgagccatat
     1501 acacatcaca cttgaaatac cgcccgcaag accagcagga ggcatgcctt gcgaattgtc
     1561 tgaacttcct catgaaaagc tgatatcccg tggctatttg ccacggctct ttcatttgca
     1621 gcgtggtgaa tcgcgacagt gggcgagcgc catccgatag ggtctccaaa gattggggat
     1681 ggcactgccc ttgcgtatga aatggaataa gaagtccgct tcgtcagaca cagaacggag
     1741 gtcaccgtgg ttattctcca gagacttcaa aactatctca aaattcatca gattccttac
     1801 gaggagatac ctcactccac cgcctacacg gctcgagagg tagccgaaaa tctccacgtg
     1861 ccggcaaaga tgtacgccaa ggtcgtcgtc gtcaaagccg acagccggtt tgtgatggcc
     1921 gtgcttcctt cttcctggct ggtcgatctg aaacgcctgg aagaggtgtt gggctattcg
     1981 cgggtaagac tggccacgga agacgagctc gcaacccttt tccccgattg tgaagtcggc
     2041 accatgccgc ccttcggtaa cctctatgga ttaccggttt acgtcgatga attgctagcc
     2101 aagaacgagg acatgtactt cgacgccggg acccacaagg gagccatcaa gctgcggtat
     2161 caggacttcg cggatcgcgt tcatccgcag gtcgcagagt ttcaccgcga accgacgaag
     2221 ttagaatatt aaggggctct tacgagcgca agcatcgagc tgtgccacag cggatacagg
     2281 aaagcccaac tgcgctaccc cgatgcgcgt cacttcgtaa cggcaggatt atacgctggg
     2341 cccgcacgac caggaccaga tcgtctgacc cgagggacca caggacctga cgccgcggtg
     2401 tgatcggcga actttgtgca g
//
LOCUS       DASSCQ010000060        23210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_41835, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000060 DASSCQ010000000
VERSION     DASSCQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 23210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23210
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41835"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            542..871
                     /locus_tag="VFH05_04215"
     CDS             542..871
                     /locus_tag="VFH05_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677446.1"
                     /translation="MTSGQDQSIPFAAQAIPFDEFLAAGKLPEGFLTSEYVAQQFVER
                     LVHYILSVPSGSYTMAQLSHLLEQLDPRAQVFFFKRLKETSPDSLKDFAPLYYGFMNE
                     FHSLLFT"
     gene            958..1329
                     /locus_tag="VFH05_04220"
     CDS             958..1329
                     /locus_tag="VFH05_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677447.1"
                     /translation="MNSTLQRAVTSFYNLVYEAQTFATTMARIDTAEQEHYAGRIEGL
                     NWVLDRCQELEDMDTNLTPSSLQRVLTEVKSDLDHELSVQRREKGRRADGREEALNFV
                     ADYLSSLITATEIESAKTPAA"
     gene            complement(1978..2268)
                     /locus_tag="VFH05_04225"
     CDS             complement(1978..2268)
                     /locus_tag="VFH05_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET6677448.1"
                     /translation="MADQSSTRRPVQIDKDLLAILCCPETKLDVSLADDALIGKVNDA
                     VSRGVLKNKAQKPVTEMLDGGLIRADKKILYPIREDIPVMLIEEGIPLDQVL"
     gene            2410..3024
                     /locus_tag="VFH05_04230"
     CDS             2410..3024
                     /locus_tag="VFH05_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET6677449.1"
                     /translation="MRILSRYFFTGLLILVPTWATFLILSALVSAVDRLIATLLGQVG
                     VIATPVIGLLLFCLLVITAGALATHVLGSQILASTERWIAQIPLVNSIYLTIKGMTDL
                     FQFRSRFGRSTVIAFPFPRDGVWALGFVMGVAPAAVQVEPSVNLLMVFVPTAIHPFTG
                     YLAFVPQPQIRPLNLLAEDAMKMEFSAGFYKPADGWLGAPKITT"
     gene            3030..3518
                     /locus_tag="VFH05_04235"
     CDS             3030..3518
                     /locus_tag="VFH05_04235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6677450.1"
                     /translation="MSLPNQLIIRAANLDDVESIITFSAAMALETENRQLDLARLREG
                     TLSLLNTPSYGFFMVAEIRDGEQRRLIGQLMITYEWSDWRNGVFWWIQSVYVDPVWRR
                     RGVFRRIHEAVASQAKADVKVCGLRLYVEQDNHTAQAVYQRVGLKRSVYKVYEEDFVL
                     SR"
     gene            3551..3925
                     /locus_tag="VFH05_04240"
     CDS             3551..3925
                     /locus_tag="VFH05_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677451.1"
                     /translation="MKCVRPLLLLLAVAILTAGCAFVRGTYGEEVQPLDLSSIKKGTT
                     TRAEVASLLGAPDRIVEVNGHEIFHYYHFDMKSGLIIIFSRTNIMSEDVFVFLDGKGI
                     VEDIVVGKKKPSPDMQFWPFGD"
     gene            4014..4376
                     /locus_tag="VFH05_04245"
     CDS             4014..4376
                     /locus_tag="VFH05_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677452.1"
                     /translation="MWQRLTLNQPIVEEQVDFIKTGETPLSEVARELGAPDEILNLKN
                     RLVARYHFIDTRYLRVDFGWGLRFVIPYYSPDLVLGGGGAATNVFEVACDSQFVVQEI
                     SFAFHQNSSRFKLWPYGD"
     gene            4502..5698
                     /locus_tag="VFH05_04250"
     CDS             4502..5698
                     /locus_tag="VFH05_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET6677453.1"
                     /translation="MIDRRLVGQQALLLVLCCLLMACMKAHASDQEEPRLRARQLGIV
                     VGTYPTGPLNAITDVPGVKVGHTTLIAGEGALKAGIGPIRTGVTVVIPRDDVWHQKVP
                     AGSFVLNGTGEMTGLAWVAESGFLEYPIALTNTLNVPRVADGVMSWMISQYPEIGITD
                     DTLTPVVAECDDGRLNDIQGRHVAEADVVNALNSAVGGPVKEGTVGAGTGMVSYGFKG
                     GIGTASRTLPESEGGYTVGVLVNANHGRKPELVVSGVPVGRLYDGSAAGPPPQPGQSE
                     GSIIIVVATDAPLDGRQLNRLAKRAALGLARTGSTARHGSGDFIIAFSTANVIPHYPQ
                     KRTYALTHLADTHLNPVITVTVEATEEAVINALTAATTVVGRDNYRVEAISLSRLRAI
                     LGTSSK"
     gene            5780..6007
                     /locus_tag="VFH05_04255"
     CDS             5780..6007
                     /locus_tag="VFH05_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677454.1"
                     /translation="MNEYQIGGGLRLLTAVEKTDAFGEFLKTRMVRALETEDPTELHY
                     LLAQLDDYHSYMWRYYKKLAKDRSERMNPGV"
     gene            6023..7246
                     /locus_tag="VFH05_04260"
     CDS             6023..7246
                     /locus_tag="VFH05_04260"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET6677455.1"
                     /translation="MMTQTIQSNTSTALLFASALSHKTDTEAAVRDLADAVRAQLGRA
                     TLELAFVFFSPHHAKKADLVATMIQEELSIEVCVGCSGEGVIADHEEFETAPALTLWV
                     ASLPRVRVTPLRMAFSQTQDQIQMAGWPEPSAEPSTFLLLADPFTTPVHDVLSVMAER
                     YPNGLALGGLCGGGHGPGENRLVRNAEVFDGGVVGVQLSGPIAVRPVISQGCCPVGER
                     YVVTRAEQNMIYELGGQPALSRLQEVFESLEGTHRRDAHRALHIGIAIDEHRSHFERG
                     DFLVRNLVGADQQSGALAIGDLVQEGQTIQFQLRDGKSASDDLHVLLAADRAKHPNPA
                     LGALLFSCCGRGEGLFGRAHHDSSVVQERMGPIPVAGFFAQGEIGPVGGKNFLHGYSA
                     SVAVFSRPDHAHDPQ"
     gene            7279..7647
                     /locus_tag="VFH05_04265"
     CDS             7279..7647
                     /locus_tag="VFH05_04265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6677456.1"
                     /translation="MPFEQTGPEGKEVTTASGLQYVDLRMGTGATAQAGQTVSVHYSG
                     WLDNGTPFDSSLERGQPFSFPLGAGRVIKGWDEGVQGMQVGGKRKLIIPSHLGYGARG
                     AGNAIPPHATLIFEVELLAV"
     gene            7672..8781
                     /gene="gcvT"
                     /locus_tag="VFH05_04270"
     CDS             7672..8781
                     /gene="gcvT"
                     /locus_tag="VFH05_04270"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET6677457.1"
                     /translation="MNSTPLIDQHRAHQAKLVDFAGWQMPIQYASVIDEYQAVRSKAG
                     LFDVSHMGRIKVSGGDSLAFLQRMTTNDVAALALCQAQYSMVCNDAGGIKDDIFVYRT
                     GETEYLLCVNASNREKILAWLSQHRATHAQCVIEDQTSALAQIAIQGPASRDILFTAG
                     AEVISDLKLHHCREITLNGISCFVARTGYSGELGYELNIPAAQAIHLWTFLLKKGECM
                     GLKPAGLGARDLLRLEMAYLLYGNDISEETTPIEAGAEWTVNFRKGYFLGKKALDDQT
                     RIGPPRRLIAFEVLEKSVPRHGFTILDAMSSQPIGIVTSGNLSPLLQKGIGLGYVPPS
                     LAEAGTAISIDIRGKKLPAVVVKPPFYRKTKTKNG"
     gene            8800..9312
                     /locus_tag="VFH05_04275"
     CDS             8800..9312
                     /locus_tag="VFH05_04275"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6677458.1"
                     /translation="MTKNLYTGKVVTLNIETVALPNGLTIELEVVRHPGAAAVVPLTA
                     DGSVVLIRQFRHAAGGFIYEIPAGKLHSGEDPRSCATRELEEEIGYKPGQLTLLSSIF
                     TAPGFTDEVIHVYKATGLTKGRQHLDRDEVLEVVEVTLEEAIGMIRAGTIRDAKTIVG
                     LQLVFTESRK"
     gene            complement(9385..9612)
                     /locus_tag="VFH05_04280"
     CDS             complement(9385..9612)
                     /locus_tag="VFH05_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677459.1"
                     /translation="MTRMLFAIMAVAVAISFSAPSFAGEEKKDKKDEKKGGHVLVYGD
                     EKKDKKDEKKGGHMDTFGDEKKDEKKGMGGK"
     gene            9832..10362
                     /locus_tag="VFH05_04285"
     CDS             9832..10362
                     /locus_tag="VFH05_04285"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HET6677460.1"
                     /translation="MPNQRPRLRDHLRPDLHVIFVGINPGLRSAAMGHHFAGHSNRFW
                     KLLFDSGMVDEPLSFREDWRLPEWRLGLTNIIDRPSAGIDDLDPAEYRAKASTLERKI
                     RRYQPRIIALLGVTIFRILFPEITRSSRLALGPTPARLAGIPVFLLPNPSGRNAHYPY
                     RVMLDAFALLRRLAGE"
     gene            10519..11298
                     /locus_tag="VFH05_04290"
     CDS             10519..11298
                     /locus_tag="VFH05_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET6677461.1"
                     /translation="MLLSGKKGLIIGVANKHSIAWAIAQSASSQGAQLFFNYQNDRLK
                     ENVEELVGTLPGSKAFPCDAADDGQIETLMADVGKEAGALDFLVHSIAYAPREELTGQ
                     FVNTTRKGFATALDVSAYSLVAVTRAAIPLMTQGGSVVTLTYLGAERVVPHYNVMGVA
                     KAALEATVRYLAHDLGPRNIRVNAISAGPIKTLAARGVSGISKMVDHHKEFAPLRHAT
                     EQGEVGDTALFLVSSLGRGITGEVIYVDGGYHILGSLASVE"
     gene            complement(11421..11831)
                     /gene="arfB"
                     /locus_tag="VFH05_04295"
     CDS             complement(11421..11831)
                     /gene="arfB"
                     /locus_tag="VFH05_04295"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET6677462.1"
                     /translation="MLHISAHVTIPDSEIEIHAMRAQGAGGQHVNKVSSAVHLRFDVG
                     ASSLPPFVKEELSKLRDSRISGEGVITIKAQQFRSQEQNREDALERLRALIQSVAIPR
                     KKRRPTKPTRSSQHKRLESKKRQSRLKTLRQSLE"
     gene            complement(11973..12197)
                     /locus_tag="VFH05_04300"
     CDS             complement(11973..12197)
                     /locus_tag="VFH05_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677463.1"
                     /translation="MSQILNMSEHTTDEELHRLTTLLLFHLVERCGGQVQFKLEDAQR
                     VRTSLTTKMVQMQVGDEVRLRIIDRLPELK"
     gene            complement(12318..13637)
                     /locus_tag="VFH05_04305"
     CDS             complement(12318..13637)
                     /locus_tag="VFH05_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6677464.1"
                     /translation="MDVINRRNFLVRSGLALGTALIASEVPSLSRSGALAATPSRSFD
                     WQAVRDQFPLSRDLIHLAGFFLASHPTPVREAIERHRRGLDADPIGYWLAHEKEQEAS
                     VLRAAADYLGVSPTEIALTDSTTMGLGLLYGGLALQQDQEILTTTHDHYSTDVSLRLR
                     AERTGATVRRISLYEELSTISRESLVKKLVGQVRDHTRIVAVTWVHSSTGLKLPIAEM
                     AKALENINASRAEKDRIIFCVDGVHALGVEDFRLSDLGCDFFVAGTHKWMFGPRGTGF
                     VWGHERAWPFANAIIPTFHSDAYDMWMRVIPPRPLPMAIYMTPGGFHSFEHRWALDQA
                     FVFHQAIGKPRVTQRIHDLNDHLKQGLAKMQHVTLRTPLSRDLSAGIVCFEVAGLKPD
                     QVVRRLRERGIVASVTPYATQYARVAPGLLNSPEEIETVLKAIDELK"
     gene            complement(13705..16593)
                     /gene="gcvP"
                     /locus_tag="VFH05_04310"
     CDS             complement(13705..16593)
                     /gene="gcvP"
                     /locus_tag="VFH05_04310"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HET6677465.1"
                     /translation="MTTPQFLSPTDIFVHRHLGPTEADVREMLALLGLPSLQALSDAT
                     VPRDIQLGRPLDIPPNRGEQAVLQELRDLAQENRIYRSYLGMGYYDCITPPVIQRNIL
                     ENPGWYTQYTPYQAEIAQGRLEALVNFQTMVADLTGLPLANASLLDEATAAAEAMAMC
                     LAITRGTQQEKKEFFVSQDCHPQTIAVLQTRAEPLDVTLHVGRTTTINFDNPQLSGML
                     LQYPTTDGYVGDYQALVQQAHNAGMLVVVATDLLALTLLRPPGDFGADIAVGSSQRFG
                     VPMGFGGPHAAFLAAKEAYKRQMPGRIAGVSKDRSGQVAYRLSLQTREQHIRREKATS
                     NICTAQVLLAIIAGMYAVYHGPDGLRRIGERVHGLAAVFAAGLQQLGFDIGSQEFFDT
                     VHVRLTKTQCEQILVRADDQGLNLRRHEDDSLSVSLDETTEEGDVRRLWEVFVGHQRL
                     PLTIQELSHTVRTSYPANLARTTPYLTHEVFNRYHAEHELLRYLHRLQSRDLSLTHSM
                     IPLGSCTMKLNATAEMLPVTWPEFARLHPFAPAEQSRGYQELFRQLEARLAEITGFAA
                     VSLQPNAGSQGEYAGLMVIRAYHRSQGEFQRDVCLIPVSAHGTNPASAAMVGMTVVPV
                     ACDKQGNVDLTDLEAKAAEHRMRLAALMLTYPSTHGVFESDVRRICQIVHTHGGQVYM
                     DGANMNAQVGLCRPGDIGADVCHLNLHKTFCIPHGGGGPGMGPIAVARHLATFLPGHP
                     LTGLGSTESIGPVSAAPYGSPSILTIPWVYIALMGRDGLMKATQVAILNANYMAKRLE
                     KHYPILYTGVGGFVAHEFILDLRQFKDIAGIEAMDVAKRLMDYGFHAPTVSFPVAGTL
                     MIEPTESESKAELDRFCHAMILIRAEIQDIIDGRQPRTNNVLKNAPHTAQAVTASQWD
                     RSYSREQAAYPAPWVKDHKFWPHVGRIDEAYGDRNLICTCPPMEPYST"
     gene            16783..17454
                     /locus_tag="VFH05_04315"
     CDS             16783..17454
                     /locus_tag="VFH05_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677466.1"
                     /translation="MRILVIEDETKVGCFIKRALEEESYAVDLCEDGAKGLEMALATN
                     YDSIIVDLMLPSLSGLDIVKGVRRERIQTPILILTAQSQVDQRVKGLDAGADDYLTKP
                     FAIDELLARVRALLRRGATDSPGILQIDDLVLNPATREVTRGGQRIELTLKEYALLEY
                     LMRHTGRVLTRPMISEHVWNQDFDTFTNVIDVYVNYLRNKVDRGRTKKLIHTIRGSGY
                     MLKAD"
     gene            17629..19032
                     /locus_tag="VFH05_04320"
     CDS             17629..19032
                     /locus_tag="VFH05_04320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HET6677467.1"
                     /translation="MPLRLRLSLWYGSALALILVAFSTVLYFVTARSLRDALDQSLEE
                     TAAAAVRSLEERGFLPLIDEDELMSQFPELARIDKFFQIFSPSGTITIRSPNVKQHEL
                     PLSRRALEVAFSGKTIFESAKYPKEPPLRLISVPIIYQGNLLYIVQVGTSMESVDQTL
                     NRLLLVLLVTMPLALAVSLAGGWFLAGRALRPVDAITLAAQRIAAGDLTQRLNVPASA
                     DEIGRLAGTFNNMIARLETSFRQIRQFSSDASHELRTPLTVMKGETELALRRPREAAD
                     YTVVLESNLEEIDRMTRIVDELLFLSRADMGEVKMERLPVKLESLLEDIHRQASLLGQ
                     ESNVQVILGLVMPAIVLGDELRLRELFLNLVDNAVKYSRPGGTVEISLTTHLNQAKLV
                     ITDQGIGISPEDQNRIFDRFYRTDDARAHTKKGTGLGLAICAWIVESHHGRIEVQSGP
                     NKGSTFTVTLPLATSPS"
     gene            19160..20680
                     /locus_tag="VFH05_04325"
     CDS             19160..20680
                     /locus_tag="VFH05_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET6677468.1"
                     /translation="MSELVRKALGGLVGMALLGTMFFVGSQSFVPFPASGSPAATPTV
                     TGPVGTLPANGFTEVAKAVTPAVVNITTVIGEKISGRRGQDEMRERMEEFFGGPNGPF
                     GPKGPSGPKFRGPEGPGEPRGFHGGGQGSGVIVSPDGYVLTNNHVIDGAREVTVTLPD
                     KREFPGKIVGTDPKTDLAVVKIEGKNLPTVAWGDASKLQVGEYVLAVGNPFGLNSTVT
                     LGIVSALGRGRMGITQYEDFIQTDAAINPGNSGGALVNTKGELVGINTAIFSQSGGYQ
                     GVGFAVPTSMGQPIYESLVKHGKVVRGFLGIGIQDLNQDLAKSFDLTDRKGALVTEVK
                     EESPAEQGGLKQGDVITSYQNSPVDDAVALQRLVTKTPVGSKVPLKVMRNGHEKDLTV
                     TVGEQPGEQKVARADEEAKDFAFAGVAVQDLDRDTAKELGLKGKTQGVVVTSVEPDSG
                     ADKAGLMPGDVIREINRQPVKSVKEFEKVSSAVKKGENVLILINRRGSSLFLSAKV"
     gene            complement(20707..22206)
                     /gene="rimO"
                     /locus_tag="VFH05_04330"
     CDS             complement(20707..22206)
                     /gene="rimO"
                     /locus_tag="VFH05_04330"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET6677469.1"
                     /translation="MNQALIQPERAKSPAPSSGSGKKTTVGFVNLGCTKNQVDSEIML
                     GTLAADGFELTGNPQKAEVVIINTCGFIEEAKQESINTILEHAKLKESGACRVLIAAG
                     CLAQRYQGDLLKELPELDGVVGTGEFGKIAGICRDLLTPKRRHQRLWISPPPYLYDEM
                     VPRLRLGKSHSAYVKIAEGCNRNCAFCAIPLMRGKQRSRPVASIMAEVQQLAEAGVKE
                     INLISQDTVNYGVDLGLRQGLVCLLRELVKVKALRWIRPFYLYPQQVTDELLDLYAGE
                     ERITKYIDMPLQHINSAMLKRMHRLGDRRVIEALVERIRTRIPGVTFRTAFIVGFPGE
                     TDHAFEELKQYVEQAEFDRVAAFLYSDEEKTPAASLDQKIDRAVMEERRNELLAVQET
                     IAAAKSMERIGSTLDVLVDGSAEEEGHWLEGRHEGLASEIDGVVYIDGNGTDSGRSGR
                     QGSGPNAGEFVTVTITGATTYDVVGHLAGESSSALERASEPRQPRGDYR"
     gene            22291..22830
                     /locus_tag="VFH05_04335"
     CDS             22291..22830
                     /locus_tag="VFH05_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET6677470.1"
                     /translation="MIRTFQGIRPTIPKSCFIEDTGIVIGDVVMGEHCSIWFHAVVRG
                     DVHFIRLGNRTNVQDLCMLHVTHDTHPLIIGNDVTIGHSVILHGCTIKDRVLIGMGAI
                     IMDGVVIGEDSVVGAGALVTGGTVVPPKSLVLGSPAKIARPVTPKELSWIKESSDNYV
                     KYAEQYMNGTPNSKTGFQP"
     gene            complement(22821..>23210)
                     /locus_tag="VFH05_04340"
     CDS             complement(22821..>23210)
                     /locus_tag="VFH05_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677471.1"
                     /translation="RFYAVDAARASGPFDHLWIVSVLNDPERFPQLAALSYGRAVPVT
                     FDAGVFRQERNIVRVIVNRCLAKLTVPGLVTTSTEEVVWIADWCHRHSIPYLVERKYY
                     PTALVGDPVCFMRIGSTRGRRQHRTQG"
BASE COUNT         4900 a         6676 c         6546 g         5088 t
ORIGIN      
        1 aagcggttcg agatgtacaa cgacaccgtg ctgggcttca acaagtcggg caaggaagtg
       61 gcgcgcatcc aggtcgaaga gccgatctat gtcagacccg ccgagcgggt gaactggctc
      121 taagatcggc gccagaggca catgttggga gaggcttccg cctctcccaa cactggcggc
      181 tgtactctac agctagggac gcttcacgaa agaggcaggg ttccacagag ccctgccttt
      241 ttctgtttct gacgactccc acatacggat cagacaaccc agctgctgca tgtaggcgga
      301 tgcaacgagc gaagctgggt ttcctccggg tgacaccagc tcgctttgct gtacgtgtaa
      361 tcagctgtca tcgacagctg ggactgtttc acaaggacac ggttccccaa gggaccctgc
      421 cacttctgat atatcggtct tttgcctcac ggcgtatgct gcttctgccc gcttgacggg
      481 ctaggggggg tccagtagag ttgtcgtaat atttctagga cttccgtgag gtgcgggcag
      541 cgtgacctcg ggtcaagacc agtcaatccc atttgcggct caggcgatac cgttcgacga
      601 gttcctggct gcagggaagc ttcctgaagg attcttgacg agcgaatatg tggcgcagca
      661 gttcgtcgag cgtctggttc attacatttt aagcgtaccc tccggcagct atacgatggc
      721 ccaactgagc cacttactgg agcaactcga cccgcgagca caggtttttt tcttcaagcg
      781 attaaaggaa accagtccgg acagcctcaa agactttgcc ccgctgtatt atggtttcat
      841 gaatgagttc cactccctcc tttttacgta agccctgcaa aatttctgct tgtcggcaag
      901 gtattctcat gtataattgt ctgatccctc catcggcctg gaatataaag gagttctatg
      961 aattcaaccc ttcagcgagc cgtcaccagt ttttataacc tcgtgtatga agctcagaca
     1021 ttcgccacca ctatggcgcg gatcgatact gccgaacagg agcactacgc cggccggatt
     1081 gaaggcctca attgggtgct ggatcgttgt caggaactgg aagatatgga taccaatttg
     1141 actccatcgt cactccagcg ggtcctcacg gaggttaaat cggacctcga ccatgaactt
     1201 tctgttcagc gacgggaaaa aggccgtcgc gccgacggcc gtgaagaagc gttgaatttt
     1261 gtggcggatt atctgtcgag cctcatcacg gcaacggaaa tcgaatcggc caagactcca
     1321 gctgcctagt gctccgccat tggggacttc gtcatcccct cttcccgtcc ggttgcatgc
     1381 cgggttgact cacgtgatgc gcgagtggat cattttcgct ctgtgcttgg ggataggcgg
     1441 ccatgtcgct ctcggcgtga tgctccatgc tcccgaattg tggccgtgga gcacggccgg
     1501 gttctatgga ctgctgagcg ggataggtat ttatgccgtt gtgcagggag gaagaggtgc
     1561 ctggagtctg ttgcggtctc ggcggaagag tggcaccggg tcaagcgacg cctggtagac
     1621 gacgtccgct gccttattga ccgagggaaa tcgctcccct acaatccatc gtatgccact
     1681 gcctccgcgt aaaccgccag gcccgggatg gcctccaggc cctccaagag ccggaaggcc
     1741 gcgtacctct gaacaagagg aacagtttag aaacctgaat gcaagcctgc tgtactgtcc
     1801 tcgctgccga gtggccactc cgacgcgcga acgactgctt ctcgtcttac cgaccggcaa
     1861 cctctatgaa tacctttgcc agcaatgcgg atcctcaacc ggatcgaaga cggagcaaga
     1921 acagggaggc atgttccggc cgtaagcctg ttgggtccga actatcagcg tgagacgcta
     1981 gaggacttga tcgagcggga taccttcttc gatgagcatc acagggatat cttcacgaat
     2041 gggatacaag atctttttgt cggccctgat caagccgccg tcgagcattt cggtcaccgg
     2101 cttttgcgcc ttattcttca acacgcctcg gctcacggca tcgttcacct tgccgattag
     2161 agcatcgtcg gccaagctga catcgagttt ggtctcgggg caacacagta tggccagcaa
     2221 gtccttgtct atctgcaccg gacgcctcgt gctcgactga tcagccatgg ccacacctct
     2281 gattgaagat tgcgggtgaa ccccaggata atgcaccctc cggctgagat caagagtgcc
     2341 tacgctctga ttgttacgcg gttctgttac actgccactc gaccgtctgc catctgaatc
     2401 acaaagacta tgaggatact ttcgcgatat tttttcaccg gcctgttgat cttggtacca
     2461 acttgggcca cttttctgat cctgtccgcg ctggtatccg ccgtcgatcg cctgatagcg
     2521 acgcttcttg gccaggtcgg cgtcatagcc actccggtca tcggtctgct gcttttctgc
     2581 cttctggtga tcacggcggg cgcactcgca acccatgtct tggggagtca gatcctcgca
     2641 tcaactgagc gatggattgc ccagattccc ctggtgaata gcatttacct caccatcaaa
     2701 ggtatgaccg acctgtttca attccgatcg cgtttcggac gtagcaccgt catcgctttc
     2761 ccgtttcctc gcgacggagt gtgggctctt ggattcgtca tgggcgtcgc gccggcggcg
     2821 gttcaagttg agccatccgt caatctgttg atggtcttcg tgccgactgc tatccaccca
     2881 ttcaccggat atctggcatt tgttccccag ccacaaatcc gcccactgaa tctgctggcg
     2941 gaagacgcca tgaaaatgga attctctgcc ggcttctata aaccggcaga cggctggctc
     3001 ggagccccca aaatcacgac atagtcctca tgtccctgcc gaaccaactg atcattcgag
     3061 cagccaatct ggatgatgtg gaatccatca tcacgttcag cgcagccatg gcacttgaaa
     3121 ccgagaacag gcagcttgac ctggcgaggc tcagggaagg gaccttgtcg ctgctcaaca
     3181 caccatcgta cggattcttc atggtggcag aaatccgcga cggagagcag cgtaggttga
     3241 tcggccaact catgatcacg tatgagtgga gcgattggcg caacggtgtc ttttggtgga
     3301 ttcaaagcgt ctatgttgac ccggtgtggc ggaggcgagg cgtctttcgg cgcatccacg
     3361 aggcagtcgc gtctcaagca aaagcagatg taaaagtctg cgggctcagg ctctatgtag
     3421 agcaagacaa tcacacggct caagcggtct accagcgtgt tggtctcaag cgatcggtct
     3481 acaaggtcta tgaagaggat tttgtcctgt caaggtaacg cctgagcaag gtacttacaa
     3541 aggaggctgc atgaagtgcg ttcgaccact gttgcttctc ctcgcggtcg ccattcttac
     3601 cgcaggttgt gcgttcgtgc gaggcactta cggagaggaa gtccagcctc tggatctttc
     3661 ctcgatcaag aaaggcacga cgacacgagc ggaggtcgca tcactgctgg gagccccgga
     3721 taggatagtc gaggtcaacg gtcacgaaat ctttcactac tatcacttcg atatgaagtc
     3781 tggtttgatc atcatctttt cacgaacgaa tatcatgagc gaagacgtgt ttgtgttctt
     3841 ggatgggaag gggatcgtgg aggacattgt cgtggggaaa aagaaaccgt cccccgacat
     3901 gcagttctgg ccgttcggcg attaacaagg gcgcaccgat gcgacatgta atagtctctt
     3961 tagctctgct agtcgtcatc atgtctgtcc caggctgtat gagaggcccg atcatgtggc
     4021 agcggctgac tctgaaccaa ccgattgtcg aggaacaagt ggactttatc aaaacgggtg
     4081 aaactccact gtctgaggtt gcgagagaac ttggcgcgcc agatgagatt ctcaatctca
     4141 agaatcggct ggtggctcga tatcatttca ttgacaccag atatctgcgg gtggattttg
     4201 gatggggact ccgatttgtc attccctatt attctccgga tctcgttctc ggcggcggtg
     4261 gggccgcaac caatgtcttt gaagtagcct gcgattcaca gtttgtggtg caagagattt
     4321 cgttcgcctt tcatcaaaac tcgtcacgct tcaagttgtg gccgtacggt gactaatgcg
     4381 tagcgatcga attgccgctt cggaccggcc cgttctatgg tccatacgac ttcctttttg
     4441 ccgaggctcg aattgctcaa gtcatagcta attcagacac actctatgca gtctatgacc
     4501 catgattgat cgtcggcttg ttggacagca ggctcttctg ttggtgctgt gctgcctgct
     4561 tatggcctgt atgaaggcgc atgcaagcga tcaggaagaa cctcgtttgc gggcgaggca
     4621 gcttggcatc gtggtgggaa cttatccgac cggcccattg aatgccatca cggatgttcc
     4681 aggagtaaaa gtcggccaca ccactctcat cgcgggcgaa ggggcgctga aggcaggcat
     4741 tggaccgatc cgcaccggcg tcaccgtcgt gattccccgg gatgacgtat ggcatcaaaa
     4801 agtccctgcc ggctcctttg tactcaacgg cacaggagag atgaccggct tggcctgggt
     4861 agctgaatcg ggctttctcg aatatcccat cgccctgacc aacacgctga atgtaccgcg
     4921 cgtagccgac ggcgtcatga gctggatgat ctcgcaatat ccggaaatcg gaatcaccga
     4981 cgatacattg actcctgtgg tggcagaatg tgatgacggc cgcctgaacg acatacaagg
     5041 ccgtcatgtt gcggaagcag acgtcgtgaa cgccctgaac agcgccgtcg gcgggccggt
     5101 aaaagaaggc actgtgggag cggggaccgg catggtctca tacggattca agggaggaat
     5161 cggcaccgcc tctcggaccc tgccggagtc agaaggcggt tatacggtcg gcgtcttagt
     5221 caacgcgaac catgggcgga aaccggagct ggtggtctcc ggggttccgg tcggacggct
     5281 ctacgatggg tcggctgcag gcccgcctcc ccagcccggc cagagcgaag gttcaatcat
     5341 cattgtcgtg gcaacggacg cgccacttga tggaagacaa ctgaatagac tcgccaagcg
     5401 agccgcatta ggattggcaa gaaccggctc aacagcccgc cacggcagcg gtgacttcat
     5461 tattgcattc tcaaccgcta acgtgatccc ccattatcct caaaagcgaa cctatgccct
     5521 cacgcacctt gcagatacac atctcaaccc tgtgatcacc gtgaccgtgg aagcgacgga
     5581 agaggctgtc atcaacgcat tgacggcggc cacaacggtc gttggacgtg acaactacag
     5641 agtggaggcc atttcgttga gtcgcctgcg cgcgattctc ggcaccagta gcaaatagaa
     5701 ctgctggtta ctctcaagcg cttgcatttt gaaccatctg ctcgctattc tcacgccgca
     5761 tctcgatcgg agggaccgga tgaatgaata tcaaatcggt ggtggcttgc gtttgttaac
     5821 cgccgtggaa aagaccgatg ccttcgggga gtttctgaaa accaggatgg tccgggcgct
     5881 cgaaaccgaa gatcccacgg aactgcatta cctgcttgcg caactggatg actaccactc
     5941 ctacatgtgg cgctactata agaaactcgc caaagaccga tctgagcgaa tgaatccagg
     6001 agtctgacca cgagaaacac ccatgatgac ccaaacaatc cagagcaata cgtcgactgc
     6061 acttcttttt gcatctgctc tctcccacaa gactgacacg gaggccgccg tacgcgacct
     6121 ggctgatgcc gttcgtgcac aactcggccg agccactctc gagctggcgt tcgtgttctt
     6181 ctctcctcac catgcaaaga aagccgacct cgtggcaacg atgattcagg aggaactgtc
     6241 tatagaagtt tgcgtgggtt gttccggcga gggggtgatc gccgaccatg aggaatttga
     6301 aaccgctcct gccttgactt tgtgggtggc ttcgctgcca cgggtccggg tgacgccgct
     6361 ccgaatggcc ttctctcaaa cgcaggacca aatccaaatg gccgggtggc ccgagccaag
     6421 tgctgaaccg agcacgttcc tcctgttggc ggatccgttc acgacaccgg ttcatgacgt
     6481 gctttcggtg atggccgagc gttaccctaa tgggctggct ctgggcgggc tgtgtggagg
     6541 cggccatggc cctggtgaaa atcggctggt caggaacgca gaagtctttg atggaggcgt
     6601 ggtcggcgtg cagctctcag gtccgattgc ggtgaggccc gtgatatcac aaggctgctg
     6661 tcccgtcggc gaacgttatg tcgtcacgag ggcggaacaa aatatgatct atgaactcgg
     6721 cgggcaaccg gctctgagtc ggctgcagga agtatttgaa tcgttagaag ggactcaccg
     6781 ccgggatgcc catcgagcct tgcacattgg aatcgcgatc gatgagcatc gcagccattt
     6841 cgaacgcggg gattttctcg ttcgtaatct ggtcggagcg gatcaacaat ctggagcgct
     6901 cgcaattgga gatctcgtac aggaaggcca gaccattcag tttcaactcc gcgacggcaa
     6961 atcagcaagc gacgatttgc atgtcctgct cgcggccgac cgtgcgaagc acccaaatcc
     7021 agccttaggg gcgcttctct tcagttgttg cgggagaggc gaggggctct tcggtcgcgc
     7081 ccatcatgac agcagcgtcg tgcaggaacg gatgggaccg attccagtgg ccgggttttt
     7141 tgcacaaggg gagatcggtc cggtgggagg aaaaaatttc ctccatggtt actcggccag
     7201 tgtcgccgtg ttctcgcgtc ccgatcacgc tcatgatcct cagtaaatgg tttacgccag
     7261 cgacacaagg ggacaactat gccattcgaa cagaccggtc ctgagggtaa agaagtaacg
     7321 acggcatctg gacttcaata cgttgacctc agaatgggca ccggagccac ggcacaagct
     7381 ggccaaacgg tgagcgtgca ttacagcggc tggctggaca atgggacgcc cttcgacagt
     7441 tcacttgagc gtggccagcc tttttccttt ccgttgggtg cgggccgcgt catcaaagga
     7501 tgggacgagg gtgtgcaggg tatgcaagtc ggcggcaaac gaaaactgat catcccctcc
     7561 catttaggct atggcgcccg tggagctggg aatgcgatcc cgcctcatgc cacgctcatc
     7621 tttgaagtgg aactcctggc tgtctgacct gcacgcatac ttctcgacgg catgaactcc
     7681 actcctctca ttgaccaaca tcgtgctcac caagccaaac ttgtcgactt cgctggatgg
     7741 cagatgccca ttcaatacgc cagcgtgatc gacgaatacc aggcagtccg ttcaaaggcc
     7801 ggcctgtttg atgtcagcca catgggacgc atcaaagttt ccggtggtga cagcctggcc
     7861 tttcttcaac gaatgaccac caatgacgtt gcagcattgg cactctgcca ggctcagtac
     7921 tccatggtat gcaacgatgc cggcggtatc aaagatgata tttttgtgta tcggaccggc
     7981 gaaaccgagt atctcctttg cgtcaatgcc tcgaatcgag agaaaatttt ggcctggctc
     8041 tcgcagcatc gagccaccca tgcccagtgt gtgatcgagg atcagacttc ggcactcgcc
     8101 caaatcgcca tccaaggtcc cgcctcacgg gatattctct tcacggccgg agccgaagtg
     8161 atcagtgacc tcaaattgca tcactgtcgc gagatcaccc tgaatggcat cagctgcttc
     8221 gtcgctcgaa ccggttatag cggagaattg ggctatgagc tgaacattcc cgcagctcag
     8281 gccatccacc tttggacctt cttactcaag aaaggcgaat gcatgggtct caagccggcc
     8341 ggtttgggag cacgagatct gttgcgtctg gagatggctt acctgctgta cgggaacgat
     8401 atcagcgagg agacgacgcc aattgaggct ggagcggaat ggacagtaaa ttttcgcaaa
     8461 ggttactttc tcggcaagaa ggcactcgac gaccagacac gaataggccc gccccgtcgg
     8521 ttgatcgcct tcgaagtctt ggagaaatcc gtaccgcgcc atggcttcac cattttagat
     8581 gcgatgtcgt cacaaccgat cggtatcgtc accagcggga acctttcgcc gctgcttcaa
     8641 aagggaatcg gcttggggta tgtccctccc tcccttgccg aagccggtac ggctatttcc
     8701 atcgatattc gcgggaaaaa gctgccggca gtggttgtaa agcctccctt ttatcggaag
     8761 acaaaaacca aaaacggttg accccgtcgg gtcttcatca tgacgaaaaa cctctacaca
     8821 ggcaaagtgg tcacgctgaa catcgagaca gtggccttgc ccaacggtct tacgatcgag
     8881 ctggaagtgg tgcgccaccc tggtgccgcg gcggtggtcc cgctcacagc agacggctcg
     8941 gtcgtcctca tcagacagtt tcgacatgcc gcaggcggat ttatctacga gatcccggca
     9001 ggaaaactcc attctggtga agacccccgt tcatgcgcga cacgggagct cgaggaagaa
     9061 atcggctata agcccggcca gctcacgctt ctctcgagca tattcacggc gcctggtttt
     9121 accgatgaag tgatccatgt gtacaaagcc accggcctga ccaaagggcg gcagcatctc
     9181 gatcgagacg aggtcctcga agtggtggag gtgacgctgg aagaggcaat cgggatgatt
     9241 cgtgcaggaa caattcgcga tgcgaaaaca atcgtgggat tacagctggt atttactgaa
     9301 agcaggaagt gaggagggta cccaaggagc catccgcctt ccggcggaag gctccttaag
     9361 ctagctagcc tcgccgaaaa gggattactt tccgcccatt cccttcttct cgtccttctt
     9421 ctcgtctccg aaggtgtcca tgtggccacc cttcttctcg tccttcttgt ccttcttctc
     9481 atcgccgtac acgaggacgt ggccgccctt cttctcatct ttcttgtcct tcttctcctc
     9541 accggcgaag gaaggagcgc tgaaggaaat cgccactgcc actgccataa ttgcgaacaa
     9601 catgcgtgtc atactgtgtc acctcctctg gttgtggaat gtatgccctt cagtgggcat
     9661 cgtgtcagga ttcagcaagc ttggtgcccg attctatccg ccctcttcct cattgcaatt
     9721 tcagtcgctt atgcgttcta caggaacgac gggggccctc atctctcggc caaactgtgg
     9781 cagaatagct gaatcgagct aaactgcctc agcccggggc gagtgaccat aatgcccaac
     9841 cagagaccta ggctgcgtga ccatttacga ccggacttgc atgtcatctt tgtcggcatt
     9901 aaccctgggt tgagatctgc agcgatgggt catcacttcg ccggtcattc taaccgcttc
     9961 tggaagctgc tctttgactc aggcatggtg gacgaaccac tgagttttcg ggaggattgg
    10021 cgcctccccg agtggcgtct gggcctcacc aacatcatcg accgacccag tgcgggaatc
    10081 gatgacctgg acccggccga gtatcgggca aaagcctcga ccctcgaacg aaaaattcgg
    10141 cgataccagc ctcgaatcat cgcgctattg ggcgtcacca tatttcgtat cttgtttccg
    10201 gagatcacgc gttcgagccg tctggcgttg gggcccactc cggcacgact tgccgggatt
    10261 ccggtgtttc tcctgcccaa tcccagcgga cgtaacgccc attatcccta ccgtgtgatg
    10321 ctcgatgcat ttgctctact gcgcaggctg gctggcgaat agtccgggag tcttttcccg
    10381 gtgatcgcac cccatgacgg gctcagcgcc ctccattctc tcgcctggaa cagttccggc
    10441 caatcatgct atcttcactg ccctgagtga ccatcgccgc gactggaata gtttctcgca
    10501 accgctggag cagaacgtat gttactgagc ggcaaaaaag gattgatcat cggcgtggcc
    10561 aacaaacaca gtatcgcctg ggccatcgct caatccgcgt ccagtcaggg tgcgcagctc
    10621 ttcttcaact atcaaaacga tcgcctcaaa gaaaatgtgg aggaactggt tgggaccttg
    10681 ccgggctcga aggcgttccc ttgtgacgcc gcagacgacg gccagattga gacgctcatg
    10741 gccgacgtcg gcaaggaagc gggcgcgctt gattttctcg ttcactcaat cgcatacgct
    10801 ccacgggaag agctgactgg ccagtttgtc aacacgacgc gcaagggttt tgccactgcg
    10861 ctcgacgtga gcgcgtattc cctggttgcc gtcaccagag cggccatccc cttgatgacc
    10921 cagggcggtt ccgtcgtgac cttgacctat ctcggggctg aacgggtcgt gcctcactat
    10981 aacgtcatgg gcgtcgccaa ggccgccctt gaggccaccg tccggtactt ggcccatgat
    11041 ctcggacccc gcaacatccg agtcaatgct atctccgctg ggccgattaa aacgcttgcc
    11101 gcccgcggcg tatccggcat cagcaaaatg gtcgatcatc acaaggagtt cgctcctctc
    11161 cgccatgcaa ccgaacaagg ggaagtgggc gacacggcgc tgtttcttgt cagctcgcta
    11221 ggccgaggca taacggggga agtgatttac gtcgacgggg ggtaccacat tctggggtct
    11281 ctggcctctg tcgaataagg catagtgaga aaccccgttc tggaaccacc gtatcctcgc
    11341 aagtcgtcac gtcctcgctc ggacgaccgt ggactgaagc ttcgggggcg ggcgggaaac
    11401 acacctgaac aaccgatggt ctattcaaga gattgacgca gcgtcttcag ccgactctgc
    11461 cgcttcttgc tctcaagcct cttatgctgg gagctccgcg tcggcttcgt cggtcgtcgt
    11521 ttcttccgtg gaattgccac gctttgaatc aatgctctga gccgttccaa cgcatcctcg
    11581 cgattctgct cctgacttct gaattgctgg gctttgatgg taatcacacc ctcgccggag
    11641 atacggctgt cccgcagctt cgacagttct tctttaacga agggcggtaa ggacgaggcc
    11701 cccacatcaa accgcaagtg aacagccgag gaaactttgt tgacgtgttg cccgccggct
    11761 ccctgagccc gcatcgcatg gatctcgatc tctgaatccg gaatggtgac gtgtgcggag
    11821 atgtgcagca tatgcgagcg agtcttgcgg ccagggggcc ctaaacggca ttctttcaca
    11881 tgacgatgaa gcgaacaagg gtcagtcagg atggtccatg accacacgca ggtcgcacag
    11941 cgcacgatac aaatgaaaaa acacttgggc gattacttga gttccggcag gcgatcgatg
    12001 atgcgaagtc tcacttcatc accgacctgc atttgcacca ttttggtcgt gagactggtg
    12061 cgcacacgtt gcgcgtcttc cagtttgaac tggacctgac ctccgcagcg ctcgaccaaa
    12121 tgaaacagca acagggtggt caaccggtgc aattcctcgt cggtggtgtg ttcactcatg
    12181 ttgaggattt gagacatgtg atcagccttc cttgtgcggg atgtgcaagc cccttatcgc
    12241 ccaatgaatg ggtgctctac aatgccccga aagaaggggt gcgcatgccg atcggattgt
    12301 tcagcttttt ccatgactta ctttaactca tcgatcgctt taagcaccgt ctcaatttcc
    12361 tccggcgaat tgagcagtcc gggcgccaca cgcgcgtact gcgtggcgta gggggtgacg
    12421 ctggcgacga tgcctcgctc acgcaatcga cggacgacct ggtcaggctt aagtcccgcc
    12481 acttcgaaac acacaatccc cgcggacaga tctcgagaca gcggcgtacg cagggtgacg
    12541 tgctgcatct tcgcaagccc ttgcttcaaa tgatcgttca ggtcatggat ccgctgggtc
    12601 acacgcggct tgccgatcgc ttggtgaaag acaaaggcct ggtccagcgc ccatcggtgc
    12661 tcaaacgaat ggaatccgcc gggagtcatg tagatggcca tcggcaacgg tcgcggcggg
    12721 atgacccgca tccacatatc gtaagcgtcc gaatgaaagg tgggaatgat ggcgttggcg
    12781 aagggccagg cccgttcatg tccccacacg aatccggttc cccgaggacc gaacatccac
    12841 ttgtgtgtcc ctgcgacaaa gaaatcacac ccgaggtcgc tgaggcggaa atcttcgacg
    12901 cccagtgcat ggactccgtc gacgcaaaaa atgatccggt ctttttcggc ccgggacgcg
    12961 ttgatgttct ccagcgcctt ggccatctcg gcgatgggca gcttcaggcc ggtgctcgaa
    13021 tgcacccatg tgaccgccac gattctggtg tgatcgcgga cctggcccac aagtttcttt
    13081 accaaactct ctcgagagat cgtgctcagc tcttcataga gcgagatccg gcgcaccgtg
    13141 gcgccagtcc gctctgctct cagccgcaac gacacgtcag ttgagtaatg atcgtgggtg
    13201 gtggtgagta tttcctggtc ttgctgaagc gctaaaccgc catagagcaa tccgagtccc
    13261 atagtcgtgc tgtcggtcag ggcgatttcg gtaggactga cgcccagata gtccgccgcg
    13321 gcgcgaagca cggatgcctc ttgttctttc tcgtgagcca gccaataccc gattggatca
    13381 gcatccaacc cccggcgatg acgctcgatg gcctcgcgca ccggcgtggg atgtgaggcg
    13441 agaaaaaatc ctgcaaggtg aatgagatcg cgtgaaagcg ggaattgatc tcgcaccgcc
    13501 tgccaatcga acgatcgaga cggtgtggca gcgagcgcac ccgatcggga gagggaaggc
    13561 acctcgcttg cgataagcgc agtcccgaga gccaaacccg agcggacaag gaaatttcga
    13621 cgattgatca cgtccatgag caggattcca tgacgtgata tctactctac tgccgtcccc
    13681 gacgtacggc aagggaccag catcctaggt cgagtatggt tccatcggcg gacaggtgca
    13741 gatcaaattg cggtcgccat acgcctcgtc gatgcgtccg acatgcggcc agaacttatg
    13801 gtccttcacc catggagcgg ggtaggctgc ctgctcccga ctgtaggagc gatcccactg
    13861 cgaggccgtc accgcttgag cggtgtgtgg agcgtttttg agcacattgt tggtgcgtgg
    13921 ctgacgtccg tctatgatgt cttgaatctc cgcgcggata aggatcatgg catggcagaa
    13981 ccggtcgagc tcggctttgg attcgctttc cgttggctcg atcatgaggg taccggcgac
    14041 cgggaacgac accgtgggag catggaatcc gtagtccatc aaccgcttgg ccacatccat
    14101 cgcttcaata ccggcgatat ctttgaattg gcgcaggtcg agaatgaatt cgtgggcgac
    14161 gaatccgccg acgccggtat aaaggattgg ataatgcttt tccaaccgct tggccatgta
    14221 gttggcgttc agaattgcca cttgtgtcgc cttcatcagg ccgtcccttc ccatcagcgc
    14281 gatgtacacc cacggtatcg tcaggatgct tggacttcca taaggagctg cagagaccgg
    14341 accgattgac tctgtgctgc ccaggcctgt gaggggatga ccggggagga acgtcgcgag
    14401 atgacgagcg acggcgatcg gccccatacc cggcccaccg cctccatgcg gaatgcagaa
    14461 tgttttgtgc agattcaaat ggcacacgtc tgcgccgatg tcgccgggac ggcagagccc
    14521 gacctgcgca ttcatattcg cgccgtccat atagacctgc ccaccgtgtg tatggacgat
    14581 ctgacagatg cgcctgacat cgctttcgaa tacaccgtgc gtcgatgggt aagtcaacat
    14641 cagggcagcc aaccgcatgc ggtgttcagc cgccttcgct tcgagatctg tcaaatctac
    14701 gttgccttgc ttgtcacaag ccaccggcac gaccgtcatc cccaccattg ccgcgctggc
    14761 tggattggtg ccgtgcgccg acaccgggat aaggcagaca tcgcgttgga actctccctg
    14821 actccggtga tacgcccgga tgaccatcaa tcccgcatat tcgccttgcg agccggcgtt
    14881 gggctgtaac gacactgccg caaacccggt gatctcggct aaacgggcct ccaattgacg
    14941 gaacagctcc tggtatccac ggctttgttc cgccggagcg aacggatgca gtcgggcaaa
    15001 ttcaggccag gtcaccggaa gcatttccgc ggtcgcattc aacttcatcg tgcaagaacc
    15061 cagtggaatc atggagtgcg tcaaggagag gtcgcgcgat tgaagccgat ggaggtaacg
    15121 gagcagctca tgctcggcat gataccggtt gaacacttcg tgggtaaggt agggagtcgt
    15181 ccgggcaagg tttgcggggt acgacgtccg gacggtgtga ctcagttcct gtatcgtcaa
    15241 cggcaatcgt tgatgcccga cgaatacttc ccacaggcgc cggacatcac cttcctcggt
    15301 cgtttcatca agtgagacgc tcagtgagtc gtcttcgtgc cggcggaggt tgaggccttg
    15361 atcatcggcc cgtacgagaa tttgttcaca ctgcgtcttc gtcagacgta cgtggacggt
    15421 atcgaagaat tcctgacttc caatatcaaa accgagttgt tgcagacctg cggcaaatac
    15481 cgccgccaag ccgtgtaccc gttcgccaat ccgccggaga ccatccggtc catgatagac
    15541 ggcgtacatg cccgcgatga tggcaagcag aacttgcgcg gtgcaaatgt tgctcgtcgc
    15601 cttttccctt ctgatgtgct gctcgcgggt ttggagggat aaccgatagg ccacctgccc
    15661 actgcggtcc ttggaaacac cggcgatgcg ccccggcatc tgacgcttgt atgcttcctt
    15721 ggccgcgaga aaggctgcat gcggcccgcc gaatcccatc gggacgccaa atcgctggct
    15781 tgatcccacc gcgatgtccg ccccgaaatc accgggcggg cgcaacagtg tgagggccaa
    15841 gaggtccgtg gccacgacaa ccagcattcc tgcgttgtga gcctgttgga cgagcgcctg
    15901 gtagtcgccg acataaccat cggtggtcgg atattgcagc aacattccgc tcaattgcgg
    15961 attatcaaaa ttgatcgttg tcgtccgtcc cacatggagg gtgacgtcca atggctccgc
    16021 cctggtctgt aagacggcga tggtctgcgg atgacagtcc tgcgagacga agaactcctt
    16081 cttctcctgc tgcgttcccc gggtgatcgc cagacacatg gccatggctt cggccgcggc
    16141 tgtcgcctcg tcgagcagag aggcgttggc aaggggcagg ccggtcaggt cagccaccat
    16201 cgtctgaaaa ttgaccaagg cttccagccg tccctgggca atctccgcct gataaggcgt
    16261 gtattgcgta taccagccgg gattctcgag aatgttccgt tgaatcaccg gcggagtgat
    16321 gcaatcgtag tagcccatcc ccagatacga gcgatagatc cgattctcct gggcgagatc
    16381 gcgaagctct tgtagcaccg cttgctcacc gcgattcggt gggatatcca aggggcggcc
    16441 gagttggata tcccgaggca ccgtcgcgtc actgagggct tgtagtgacg gcaaacccag
    16501 caaggccagc atttctcgca catcggcttc cgtggggccg agatgacgat gcacgaagat
    16561 gtcggtcggg cttaaaaact ggggagtcgt catgacgggt aaataggcgc tcgagccggt
    16621 ggtttaaccg atgcctccga acattatcat ggccgacctc acttctccaa gccttcagta
    16681 cagacagccc acgatccgtc ccgtcatgcc caaccccgcc tcgggcccag gttccactgg
    16741 aaatcgccgg cgtccggttt gttataatcc tccattgccg ccatgcgaat cctcgtcatt
    16801 gaagatgaaa ccaaagtcgg ctgcttcatc aagcgggcgc ttgaggaaga gagttatgcg
    16861 gttgatttgt gcgaagacgg tgccaagggc ctggagatgg cccttgccac gaactacgac
    16921 tcgatcatcg tcgaccttat gcttccctcc ctgtccggct tggacatcgt caaaggcgtc
    16981 cgtcgagagc gcattcagac gccgatcctc attttgacgg cgcaatctca agtcgaccag
    17041 cgtgtgaagg ggttggacgc cggggcggac gactatctta ccaagccgtt tgccatcgac
    17101 gaactgttgg cccgcgtgcg cgcactgctg cgccgtggag cgaccgacag tccgggcata
    17161 ttgcagatcg acgatctggt cctgaaccct gccacacggg aagtgactcg tggcgggcag
    17221 cgcattgagc tcaccttgaa ggagtatgcg ctgctcgagt atctcatgcg ccatacggga
    17281 cgcgtgctga cccgtccgat gatctccgag catgtttgga atcaagattt cgacacgttc
    17341 acgaacgtga tcgacgtgta cgtgaattat ttgcgcaaca aggtcgaccg cggacgtacg
    17401 aaaaagttaa tccataccat ccggggcagt ggctacatgc tgaaggccga ctagcaggat
    17461 gctgccgatc tcaattgttc cttcgcgccc gtctcagtcc gtttttgtgg ctcaccggtc
    17521 cctcggaccg ttggctccac gcaacacgat acgccgtgct ccaggtctcg tctttacgcc
    17581 gacgcatgct cctcctgcat gccacgttgg ctcttcggga gtactgtgat gcccctacgc
    17641 ctccgactga gcctttggta tggcagcgcc ctggcgttga tcttagtggc cttttccacc
    17701 gtcctctatt tcgtcacggc gcgcagcctt cgggatgcgc tcgatcaatc cctggaagaa
    17761 accgcagcgg ccgctgtgcg ttctctggaa gaacggggat ttctcccact catcgacgaa
    17821 gacgagctca tgtcgcaatt tcccgagttg gcgcgcatcg acaaattttt ccagatcttc
    17881 agcccctcag ggacgatcac gatccgctct ccaaacgtca agcaacacga attgcctctg
    17941 agccgacgcg cattggaagt ggccttttcg gggaaaacga ttttcgaatc ggccaaatat
    18001 ccgaaagaac ctccccttcg gctgatctcg gtgccgatca tctatcaagg gaacctgctc
    18061 tacatcgtgc aggtcggcac atcaatggag tcggtcgacc aaacgctaaa ccgtctcctg
    18121 ctcgttctgt tggtcaccat gccgcttgcg ctggccgtct cactggccgg agggtggttt
    18181 cttgcgggac gggcgttgcg tcccgtcgat gctattacgc tcgccgctca acggatcgcg
    18241 gccggcgatc tcactcaacg gttgaacgta ccggcctctg ccgacgaaat tggccgtctt
    18301 gccggcacct tcaacaatat gatcgcgcgc ctcgagacct ctttccggca gattcgtcag
    18361 ttcagcagcg acgcttctca tgaacttcgg accccgctga cggtcatgaa gggtgaaacc
    18421 gagctggctc tgcgccggcc ccgtgaagcg gctgactaca ccgtcgtgct ggaaagcaat
    18481 ctggaagaga tcgaccgcat gactcgaatc gtggatgaat tgcttttttt gtcccgtgcg
    18541 gacatgggcg aagtgaagat ggagcgcctg ccggttaaac tcgagtcctt gctggaagac
    18601 attcatcgac aagcgtccct tctcgggcag gaaagcaacg tacaagtcat tctcggcctc
    18661 gtgatgccgg cgatcgtctt gggcgatgaa ttgcggctgc gcgagctctt tctgaacctc
    18721 gtggacaatg ccgtcaaata ttctcggcct ggcgggaccg ttgagatatc tctgacgacc
    18781 catctcaatc aggcgaagct cgtcattacg gatcaaggca ttgggatttc tcccgaagat
    18841 caaaatcgga tcttcgaccg cttttaccgc accgacgatg cccgcgccca cacgaaaaaa
    18901 ggcaccggcc tggggctcgc catttgcgcg tggatcgtcg aatcccatca tggacgcatc
    18961 gaggtgcaga gcgggccgaa caagggctcg acgtttacgg taaccctgcc gctggcgact
    19021 tcaccctctt agacactttt aataccctcc taatcggtct ctcattcctg cttgctaccg
    19081 tcctctgtag ggattttcgg ctgccgtgcc gcatgtttca ctttcaatct ctatcacgtt
    19141 caatctctaa ggaggatcta tgagtgagct ggttcgtaaa gcgctcgggg ggctggtcgg
    19201 tatggccctg ctgggcacga tgttcttcgt cgggagtcag tcatttgttc cgtttccggc
    19261 gtccggttca ccggccgcca caccaaccgt gactggaccg gtcgggacgc ttccggccaa
    19321 cggattcacg gaggtggcca aagcggtcac tccagccgtc gtcaacatca cgaccgtcat
    19381 cggagagaag atctccggcc gacgcggtca ggacgaaatg cgggaacgca tggaggaatt
    19441 tttcggaggc ccgaacggtc cctttggacc gaagggtcca tctggaccga aattccgggg
    19501 acccgagggc cccggtgaac cgcgtgggtt ccatggcggc gggcaaggtt ccggtgtcat
    19561 cgtctctcca gacggctacg tgttgaccaa caaccatgtg atcgacggcg cccgcgaggt
    19621 gaccgtgacc ctcccagaca agcgggagtt cccaggcaaa atcgtgggca cggatcccaa
    19681 gactgatctg gccgtcgtga agattgaagg caaaaatctg ccgaccgtag cctgggggga
    19741 cgcctcgaag ctccaagtcg gagaatatgt cctcgccgtc ggcaatccct tcggcctcaa
    19801 ttccacggtg acacttggca ttgtcagtgc gttaggccgt ggccgcatgg gaatcactca
    19861 atatgaagac ttcattcaga cggatgccgc cattaatccc ggcaattcgg gtggcgcctt
    19921 ggtgaatacc aagggagaac tcgtgggcat caacaccgcg atcttttctc aaagcggcgg
    19981 ctaccagggc gtgggcttcg cagtcccgac cagcatgggc cagcccatct atgagagtct
    20041 cgtgaaacac ggcaaggttg tccgaggatt cctcggcatc ggcattcaag acctcaacca
    20101 ggatctggct aagtcctttg acttgaccga ccggaagggc gcattggtga ccgaggtgaa
    20161 ggaggagagc ccagccgagc agggcggcct caaacaaggc gatgtcatca cgtcctatca
    20221 gaactccccc gtggatgacg ccgtggcact gcagcgcctg gttaccaaga ccccggtcgg
    20281 atcaaaagtc cccttgaagg tcatgcgcaa cggccatgag aaggatctca ccgtcacggt
    20341 cggtgagcag cctggtgagc agaaagttgc aagggctgac gaggaggcaa aagacttcgc
    20401 ctttgcgggc gtggccgttc aagacctcga tcgtgacacc gccaaggagc tggggctcaa
    20461 aggcaaaacg caaggtgtcg tggtcacaag cgtcgaaccg gacagcggag ccgacaaggc
    20521 cggcctgatg ccgggggatg tgatccgaga aatcaaccgg caaccggtga agtcggtgaa
    20581 ggagtttgaa aaggtgtctt cggcggtcaa gaaaggcgaa aacgtcttga ttctgatcaa
    20641 ccgtcggggc tcgtccctat tcctcagcgc gaaagtgtaa tcgcgagtcg ccccggggaa
    20701 ctgtcctcac cgatagtctc ctcggggctg acgcggctcg gacgcccgct cgagggctga
    20761 ggaactttct ccggcgagat gtccgacaac gtcatatgtg gtggcaccgg taatcgtcac
    20821 cgtgacgaac tctccggcat tgggaccgct cccctgccgg cctgagcgac ctgagtccgt
    20881 gccgttaccg tcgatataga ccacgccgtc gatttcagac gccaggcctt catgtcggcc
    20941 ttccaaccag tgcccttctt cttcggcgga accatccacc agcacgtcga gagtcgatcc
    21001 gatacgttcc atgctcttcg ccgccgcaat ggtttcctgc accgccagca gctcgttacg
    21061 acgttcctcc atcaccgcac gatcgatttt ctgatcaaga ctggctgcgg gtgtcttctc
    21121 ttcgtccgaa tagagaaatg ccgccacacg atcgaactcc gcctgctcga cgtactgctt
    21181 gagttcttcg aaggcgtgat cggtttcgcc gggaaatccc acaatgaagg ccgtgcgaaa
    21241 ggtgacgcca gggatgcgtg tccggatgcg ttccaccaac gcctcgatca ctcgccggtc
    21301 gcctagccga tgcatccgct tgagcatggc actattgata tgttgcagtg gcatgtcgat
    21361 gtacttcgtg atccgctctt ctccagcgta gagatcgagt aattcgtccg tgacttgctg
    21421 cggatacagg taaaacggtc tgatccaacg cagcgctttc accttcacca gctcgcgcaa
    21481 caaacacacg aggccctgac gtaggccgag atcgacgcca tagttgaccg tgtcctgcga
    21541 gatcagattg atctccttga cgcctgcttc cgccagttgc tgcacctcgg ccatgatgga
    21601 agcgaccggc cggctccgct gtttgccccg catcagcgga atggcgcaga aggcacagtt
    21661 acggttgcac ccttcggcaa tcttgacgta ggcgctgtgc gatttcccga gacgaaggcg
    21721 tggcaccatc tcgtcgtata aatagggagg cggactgatc cagagccgct gatgtcgtcg
    21781 tttgggcgtc aagagatcgc ggcagatccc ggcgatcttt ccgaattctc cggtgccgac
    21841 gacgccgtcg agttccggca gctctttaag cagatcccct tgatagcgct gtgccagaca
    21901 accggcggcg atcaatactc tacaggcacc agattctttc aacttggcat gctccaggat
    21961 cgtattaatc gactcctgtt tggcttcttc gatgaatccg catgtattga tgatgacgac
    22021 ttcggccttt tgcgggtttc cggtcaactc aaacccgtct gcggccagcg tcccgagcat
    22081 gatctccgaa tcgacctgat tcttcgtgca gcccaggttg acgaagccca cggtagtttt
    22141 tttcccggat cctgaggacg gtgcaggcga cttcgcacgc tccggctgaa tgagagcttg
    22201 attcataagc tcatcagtct acgagacggc tcaaaagact gtcaacgagc cacttgcagg
    22261 tgaacggctg attcgcctat ggtgcgaccg atgattcgaa cctttcaagg catcaggccc
    22321 accattccga aatcctgctt tattgaagac accggaatcg tcatcggcga tgtggtgatg
    22381 ggtgagcact gtagtatctg gtttcatgcg gtcgttcgcg gtgatgtcca ctttattcgt
    22441 ctggggaacc gtacgaacgt ccaggatcta tgcatgttgc acgtcacgca cgatacgcat
    22501 ccgctgatca tcggcaacga cgtcaccatc ggccacagcg tgatccttca tgggtgcacg
    22561 atcaaggacc gcgttctcat cggcatgggc gcgattatca tggacggggt ggtcattggt
    22621 gaagactcgg tggtcggtgc gggtgccctg gtgactggag gcactgtcgt accacccaag
    22681 agtcttgtcc tggggtcccc ggcaaaaatc gccaggccgg tgacacccaa agagttgtcg
    22741 tggatcaaag aatcgtcgga caactatgtg aaatatgcgg agcaatacat gaacggtacg
    22801 ccgaactcca aaaccggttt tcagccctga gtgcggtgct gacgtcttcc gcgtgtgctg
    22861 ccgatcctca taaaacagac cggatcgcca accaaggccg tcggatagta ctttcgttcg
    22921 acgagatacg ggatgctatg gcgatggcac cagtccgcga tccagacgac ctcctctgtg
    22981 gacgtcgtga cgaggccagg cacggtaagc ttcgcgaggc agcggttcac gatcactcgc
    23041 acgatgttcc tctcctgccg aaacacaccg gcatcaaagg tcacgggcac cgcccggcca
    23101 tacgacaacg ccgctagctg cgggaaccgc tccgggtcgt tcaagacgct cacgatccac
    23161 agatggtcga agggtccgga cgctcgagcc gcatccaccg cataaaaccg
//
LOCUS       DASSCQ010000061        14195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1436491, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000061 DASSCQ010000000
VERSION     DASSCQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436491"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..471)
                     /locus_tag="VFH05_04345"
     CDS             complement(184..471)
                     /locus_tag="VFH05_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677472.1"
                     /translation="MSYTLLNIDLADLTAIKITCAQCHASAEFPVARLQSDSPERCFH
                     CRAMWFAAKSPQATALEHLFRALGELRGDAVHECRVHFVIDPGLPRTKVPY"
     gene            complement(562..1491)
                     /locus_tag="VFH05_04350"
     CDS             complement(562..1491)
                     /locus_tag="VFH05_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677473.1"
                     /translation="MFHAWRSLIQPIQTFFRETAGGFYHVVVIALSAGIALLLPAGAR
                     QFLSFWSRIEQDKLSLILIEMIVAVLLIGCFSYVRRSRRDRVLAKAAAGAGLISFFPR
                     HEPRAHRHIARLKEENTTGRTIMVIGSSGYSSFVDQVGDLSSVLDTCLGAKILLVNPY
                     SQEATTRIAAIDHPEYTLHAFREELRQSIALLKRLRAMGKAVKLKLYADAPLVKMIIL
                     GDYLWLQHYHAELDVRTMPEYLLQHNRQHHGLYSLYAHYFAQRWQRVEIPEYDLDKDE
                     LVYRNSAGVETRRERFEIVSADEGQLYQVAGSR"
     gene            1943..2545
                     /locus_tag="VFH05_04355"
     CDS             1943..2545
                     /locus_tag="VFH05_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6677474.1"
                     /translation="MNGYVRLTCLLAVSCMVLWTAGCSKKSVQSDGRTSSAARPPVQE
                     PLPNLSLDDPSGTERLSSGIAVAKNDPATAKRQMDQVRAEDAATAAAGLRDVFFGYDS
                     WTLSDDGRQALSRDAEWLRVHPAAQLKVEGHCDERGSSTYNFVLAEKRAKSILNYLSD
                     LGIKRERIVAVSYGKERPFCKDRSEACYQQNRRGHLVVRP"
     gene            complement(2785..3984)
                     /locus_tag="VFH05_04360"
     CDS             complement(2785..3984)
                     /locus_tag="VFH05_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET6677475.1"
                     /translation="MTRLRLFVLMLGLLLTAASPASAELLALLNYESKPDQPVRREGI
                     AIMDIDPASADYGKILMEIPLPADLVAHHIFFNRDLTKAYITALGKPVLHVFDLTHFP
                     YKLREIAVPDCQVLEDLAVSEDNKKWYLTCMGSSNVIIGDAVNDKPIKTVSGTDPHAS
                     ILYPHGVAIHNGIDRVVVTSTVKPDMSEAGDSVTVIEASTGKVLSTHKVSNKPDPAKA
                     APVEVMFSPKSNPPVIHITNMLEATLWAGVWDPKKKEFSFTQIDDFGPRQQGMPLEML
                     YNAKGDRLFVTTAKPGFVNLYDNTDPRRPKFLKTIPAAEGAHHSVLSPDERYLFVQNS
                     LLNLEGMSDGSITVIDLKDDKVLGSIDTLKKAGFNPNCIMLLPNHFQKGGLPNQSQKS
                     RLRADAQ"
     gene            4428..5069
                     /locus_tag="VFH05_04365"
     CDS             4428..5069
                     /locus_tag="VFH05_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677476.1"
                     /translation="MPSSSPDVYIEEHNVVIAPGGLAARAWPVNWSAIWVGALASLAV
                     ALIFGLIGTAIGAHQVGKKVVNLHDLTLGGLIFSVAGAFFAFVVGGWVASSIAGLRRA
                     EDAALHGTIAWLAALPLFLFLAALGAGSFFGGWYGGLAGTPVWATSSTVTADPNAAMV
                     ARNSAIGAVTALLIGLVGSLIGGWLASGEPMSLTYRRMKEDDDVPGRRRARAA"
     gene            5356..5952
                     /locus_tag="VFH05_04370"
     CDS             5356..5952
                     /locus_tag="VFH05_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6677477.1"
                     /translation="MIREFHLADVLTLANAACGLAAVLCAMLYMGSELPAHYFIAAAL
                     TPAALVFDWLDGRVARFRHTHSALGRELDSLADIISFGVAPATLGFAAGLRGAWDWLA
                     LIYFVCCGVSRLARYNVTAERLSEGSDKVAYFEGTPIPTTILLTAVLAVAGWQDRIGE
                     RLYWGSWSLGPWELHPLSLLFVISGSLMISKTLRIPKI"
     gene            6088..6480
                     /locus_tag="VFH05_04375"
     CDS             6088..6480
                     /locus_tag="VFH05_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677478.1"
                     /translation="MTRAWIALAVCLSLGLAGCGGEKAEIKEDRLTVGKVQGEIKVGM
                     PASQVAELLGSPNIVTTDDKRREVWIYDKVSTDRVDTASSSFGGIIILGGSSSDRSSS
                     QRQRTLTIIIKYDEEKKVRDFAYNSTQF"
     gene            6500..7072
                     /locus_tag="VFH05_04380"
     CDS             6500..7072
                     /locus_tag="VFH05_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677479.1"
                     /translation="MSGWAPVATALLFSSQLAGCTTPSDPSELFQLTPESAINRAMQT
                     RLFETSDQTELLSASAAVLQDLGFQVEESVQDVGFLRATKERSARQYGQDIGRFLIFV
                     LSLGHVIIPVDLHQKIAAVLVERPINPDGTRREVRIVFYRVIWKGDGHADRQYVPPGE
                     QHMEMLRDAALYQQFFAKLSKAVFLEPFTL"
     gene            7091..7525
                     /locus_tag="VFH05_04385"
     CDS             7091..7525
                     /locus_tag="VFH05_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677480.1"
                     /translation="MMRRAWWGTLAIAGTLFVQGCETPQPTQDLLAPTEAQMKMRSFQ
                     TRTFDLEDRLLAMRAVIAALQDLGFIIERANESLGLVTAARFAEPNYYDVVGITVTVR
                     QETKERMMIRANAIHNNKPIEDPKVYQNFFATLERSLFVTKT"
     gene            7528..8142
                     /locus_tag="VFH05_04390"
     CDS             7528..8142
                     /locus_tag="VFH05_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677481.1"
                     /translation="MEVGPKVDRKAIGTGGVRRTAVMLAVVLWLPLTGCPTPYEWRHE
                     GQWDSRDQVWLSEQSQVKLRAAQSRVFDTTDRRRTIEAVIDTMQDLGFMVEVLDENLG
                     IVSGKRFEPIETSSFFNDPFYHLYSDESLLVFTKSYRTWGPFWHRSDLVRLTITVRKR
                     NEAQLVVRASAQYYLKAVEDPLPYQRFFRTLEQALFLQAKPPVE"
     gene            complement(8426..10966)
                     /locus_tag="VFH05_04395"
     CDS             complement(8426..10966)
                     /locus_tag="VFH05_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677482.1"
                     /translation="MGSLWTRVKDACGWNKLFHAEIPMEHSAGREAAEAYDDWRRNFI
                     RERLHVLYYLGFIANPAFIALDYLQHRDYLIPLFIVRVVLQLGLLGGFLLFKFGPKSI
                     PPNWFVILWVVVANLCVTQMTVILGGFSSQYYNGLNLVYLTGAVIVPVSWPSHLAAQL
                     VTLSSYYVSNSLGPQPDFNAAIENSFFLIWTCVALLFSVGLYERLQRAEFFARVSERH
                     ARQELEISNQKLLELDRLKSDFFANISHEIRTPLTLTLGAFQTLLKTKLSEEANDLVK
                     GGVRNASRLLFLINELLDLAKFDSGRAALRKRSLDFAALVRGVVTNFDSSPIRRIHLK
                     GLTEPVALEGDSRQLKKVLYNLLSNAFKFSDPQTGQVWIRLASKDQSIELEVEDNGIG
                     IPRQQLARIFDRFTQVEGGATRRYEGSGIGLALVKEIVALHGGTIAVESDLGRGSIFT
                     ITLPRGNVNKDHVLGVSDEEDDQTFLPLQPIERQVDTHTPPTLKLNAPLVLVVDDNAD
                     MRSYVERILRKDYRVVLAKDGAEAFEQAQRQHPELIVTDVMMPKMSGHDLLQAVRHHK
                     ALRSVPIIFLTARAGTEARVESLDAGADDYLSKPFDELELLARVGNLIRTRAQERELV
                     QLQKEKISRFLPPNLADLIMSGEHDDFLRGHRQEISVLFIDLRGFTAFVELAAPEEVM
                     TVLREYQAVMGRLVSEYGGTLERFVGDAVMVYFNDPLPCPNHAEQAVKLAVAMRQAVE
                     SLRHEWKSHGIDLGAGIGIATGYATIGAIGFEGRKDYAAIGPVTNLAARLCSEAQHGQ
                     ILISDRVISYVKGLVTMESVGELKLKGVQKPVAVAQVIGTAVNSSIGN"
     gene            complement(11027..12397)
                     /locus_tag="VFH05_04400"
     CDS             complement(11027..12397)
                     /locus_tag="VFH05_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HET6677483.1"
                     /translation="MVPTRKLSRCSLLLVALSSPLFSTTTALALNGPQFIGFSAESTA
                     LAGSGHVAVADTSAINTNPAAMSLIQATRLDVTAGFIQAFLHHSDAFGNNNVAGQNNA
                     YAIGNLGVATRLSSVPGLTIGAGIFTQGGFGSDYRNLTTAFGTQDAGSSFFRYLKFAI
                     GLSYEATDKLSFGIAPSVGYSDVSLRLFPGTSVAPSPGLPSGFAGFNIRDSCARNGGL
                     GALGGDCPSDVVFAVKIGAMYRALPWLTVGATYSSPVSFNYTGGQANLNFSSFGLGTV
                     NYHARVEGIKWPQQVDVSMAAKPTEHLMVALTTSWLNWASTNTVVITGTNPNNALAPA
                     QVNLNIPFNWKDQVIIALGFSYSVLQESSWKDKDRLVLRAGYNYSNNPVPKETLSPLW
                     PLINEHHFSAGIGFRFTERWAYDFTGLYAPKNSVTYTNPSLPFGPNATESISGYLISN
                     TLSYRF"
     gene            complement(12657..13952)
                     /locus_tag="VFH05_04405"
     CDS             complement(12657..13952)
                     /locus_tag="VFH05_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677484.1"
                     /translation="MFIEPKEERRRSERRYRDKRLLRCEREFQAAVRITSALFEHLAL
                     DELAVKALQIAIEVANAECGSILLADPPSQQLIFHHSIGASSVPSGTAISWNEGLAGT
                     VFQSGEPIVIADAQKDPRHLESIDQLTGYHTRDLITLPLKRWEGDPIGVLQVMNKREG
                     LLNGEDLAILTIVSAITASSIEQARLFQEAKLAEVARLLGDIGHDIKNLLMPVVCGTD
                     LLQSDLHDLLGDALKHEDKQAKERFDRCQDVIQMVDNSTRRIQDRVKEIADCVKGLST
                     PPEFKPCQLNRVVEEVLHTLKWSADQKGVSICTSVLDLTAEFVADERRLFNALYNLIN
                     NAIPEVPAGGTITIAAKEEPVGVSVHLTVTDTGKGMPQDIRDALFTPAARSSKRGGTG
                     LGTKIVKDVVDAHKGKITVESELGRGTTFHIYLPLRPPP"
BASE COUNT         3153 a         3837 c         4032 g         3173 t
ORIGIN      
        1 ccgaccgagt tggactggat ccttggcctg gcggtgcgct ggcgggatta cgaagtgagc
       61 ctctaccgtg agcaggaccg tccgctcgac cgctcggggc tcattcagga atattgggcg
      121 gtgcagctgc ggtatgcctt cgacgtacaa aaaagatgag agtcgcgtcg ccgatgattc
      181 gcgctagtac ggcacttttg tgcgagggag gccgggatca atcacgaagt gcacccggca
      241 ttcatgcacc gcatcgccgc ggagctcgcc cagcgcgcgg aagagatgct caagcgctgt
      301 ggcctgcgga ctcttggcgg caaaccacat tgcgcggcag tgaaagcatc gctcgggcga
      361 gtcgctttgg agccgtgcca caggaaattc cgcggacgca tggcactggg cgcaggtgat
      421 cttgatggct gtgagatcgg ccagatcaat attcaacaag gtgtaactca ttccagatcc
      481 tttcatcgca ccgctagaaa gtcgcgcatc cgtctagatc caggtcctgt accaggacgc
      541 acgggtgtga ggccgtgcaa tctacctgga gcctgccacc tggtacagct gcccttcatc
      601 cgctgagacg atctcaaacc gttcccttcg tgtctcgact ccggccgagt tgcgatagac
      661 caactcatcc ttgtcgagat cgtactcggg aatctcgacg cgttgccatc gctgtgcgaa
      721 gtaatgggca tacagtgagt acaagccgtg atgttgccga ttatgctgaa ggaggtactc
      781 cggcatggtg cgaacgtcca gttcggcatg gtagtgctga agccacagat aatcaccgag
      841 aataatcatc ttgaccaagg gtgcgtctgc gtagagtttc agtttgacgg ctttccccat
      901 ggcccgcagc cgcttgagca gtgcaatgct ttgccgcagt tcttcacgaa aggcatggag
      961 agtatattcg gggtggtcaa tggcagcaat gcgggtcgtc gcctcctggc tatacggatt
     1021 cacaagaaga atttttgcac caaggcaggt gtcgagcacg gatgacaaat cgccgacttg
     1081 gtcgacgaag ctactgtatc cgctggaacc aatcaccatg atggtgcggc ctgtcgtgtt
     1141 ctcttcctta agccgggcga tgtgtcggtg agcgcgaggt tcgtgtctgg gaaaaaagga
     1201 gatcagccct gcgccggcgg cggctttggc caaaacacgg tcccgccggc tacgccggac
     1261 atagctaaag cagccgatca acagcacagc cacgatcatc tcaatgagaa tcagtgaaag
     1321 tttgtcctgt tcgattctgg accagaagct gagaaactgc ctggccccag caggaagaag
     1381 gagggcaatt cctgcgctta aggcgatcac gacgacatga tagaagcctc ccgccgtctc
     1441 acgaaaaaac gtctgaatgg gctgtatgag tgaccgccat gcgtggaaca aagatacacc
     1501 tcttccggag ttcgtgttga gacagaacgt catgctctgt cagatgtcgg ggatcgtagg
     1561 tccgtgccgg gagggtgctc gccagagaca gaacgatctc gatcaatgcg tcgacatgca
     1621 gggaactgaa ctggagcccc gccaaaggtg gaggcggttt cacagtcaat ggcaacctag
     1681 catggttgtg gaagtgacac aagctcttat gtcaattcag aaatgactgg gcctcatcgt
     1741 cggtccgcaa gcggcagaag tacgagccgg gcccctcgaa gtttacctga tcttaacttt
     1801 tgctagagtg gctcaagtag tgccttcact cgccttacat attgccctgt tgcgttcatc
     1861 ttggtacact ccggcgggcg cgaaacgaag agccgctgac acaagcgggc aagcgtctcg
     1921 tgatgttcac caaggaggct gtatgaatgg ttatgtgcga ctcacctgtt tgctggcggt
     1981 aagctgtatg gtgttgtgga cggcgggatg ttccaaaaaa tcggtgcaat cggatgggcg
     2041 cacgtcaagt gccgcacgtc cgccggttca agagccgctt cccaacttgt ctctggacga
     2101 tccgtcaggc accgagcggc tgagttcggg aatcgccgtg gcgaagaatg atccggcgac
     2161 ggccaagcgg cagatggacc aagtgcgtgc tgaagatgcg gccacggcgg ctgccggcct
     2221 ccgcgacgtg tttttcggtt acgatagttg gactctgagc gacgacgggc gacaggcgct
     2281 cagccgcgat gccgaatggc tgcgcgtgca tccggccgcc caactcaaag tggaggggca
     2341 ctgcgacgag cgcgggagtt ccacctacaa cttcgtgctg gccgaaaagc gagccaagtc
     2401 catactcaac tatctgtcgg atctgggtat taaacgggag cgcatcgtgg ccgtgtccta
     2461 cggcaaggag cgcccattct gtaaagaccg ttcagaagcc tgttatcagc agaatcgtcg
     2521 gggccatctc gtcgtcaggc cataaacggc gactgacgat catgtagtga cgcgagaccg
     2581 tcgtgggaga cgcgcacgtc ccgggtattc gattgcagag caatcggggg cgggtgggtg
     2641 aaaaaagatt gcgcatggct ggtcgggtga gaatggccat gacggtctcg gtcaatcgtt
     2701 cgcgcgtcaa gtcacgctgt tcctgccgcc gcgaaatgcg gcaaaaattc ccatcgctcg
     2761 aatcaggtca cgctcccgcc ggccctactg ggcgtcggcg cgcagtctgc ttttctgaga
     2821 ttggttcggc agcccgccct tctgaaaatg attcggcagc agcatgatgc agttaggatt
     2881 gaagcccgcc tttttcaacg tgtcgatgct gcccaacacc ttgtcatcct ttaaatcgat
     2941 caccgtgatg gatccgtcgc tcatgccttc gagattgagc aggctattct gcacgaagag
     3001 atagcgctcg tcgggtgaga gcacgctgtg atgcgcgcct tccgccgcag ggatcgtctt
     3061 cagaaacttg gggcggcgcg ggtcggtgtt gtcatataga ttgacgaatc ccggcttcgc
     3121 ggtcgtgacg aacaagcggt caccctttgc gttgtacagc atctccaacg gcatgccttg
     3181 ctgccgtggg ccaaaatcgt cgatctgggt gaaagaaaac tccttcttct tgggatccca
     3241 tacgccggcc cagagcgtgg cttcaagcat attggtaatg tggatgaccg gcggattgga
     3301 cttcggcgag aacatcactt caaccggggc cgccttggcc gggtcaggct tgttcgacac
     3361 cttgtgcgtc gacagcacct tgcccgtgct cgcttcgatg accgtcaccg agtctcccgc
     3421 ctccgacata tccggcttga cggtgctcgt caccaccaca cggtcgatcc cgttgtgaat
     3481 ggccacgccg tgtggataga ggatcgaggc atggggatcc gtcccgctca ccgtcttgat
     3541 gggcttgtcg ttgacggcat cgccgatgat cacgttgctc gagcccatac aggtcagata
     3601 ccacttctta ttgtcttccg acaccgcgag atcttccaac acctggcagt ccggcaccgc
     3661 aatttcgcgc agcttgtagg ggaagtgggt gaggtcgaac acatgcaaga ccggcttccc
     3721 taaggcggtg atataggcct tggtcagatc gcggttaaaa aagatgtggt gggcgacgag
     3781 atcagctggc agtggaatct ccatgaggat cttgccgtag tcggccgagg cgggatcgat
     3841 gtccatgatg gcgatccctt cgcgtcgcac cggctggtcc ggcttactct cataattgag
     3901 cagggcgagc agttccgccg aggccggcga tgccgcggtg agcaacaatc ctagcatcag
     3961 aacgaacaat cggagccgtg tcatgggagc ctccctttga gtagtctgag ggcgaaaaga
     4021 acgataacgg tacgcttctc tcggcagaga agcaatgtga gagttgaccg cgctgtccat
     4081 gatgtcctcc gggttgatgg cctttgccgg ggctacgctg tcgggccgca ctatagagcg
     4141 aaccgccttt tcacgtctta tccgccagtc cgtttctctt aaccggcgcc gcttctgcct
     4201 gaaccgcagc cggcccgacc agggttcctt ctaggagggt gtgacagacg agttgtcttc
     4261 cgcaagcaca gggtttccgt gactacgcgg gtcggaaagc tctcggggaa aaacactagt
     4321 ttgtagaccc gcgaagttac gccacaatcc cccataccag cgcttgagct tgattggtcc
     4381 tggctttgca accctctcat gtgaaaggtc tttaacaagg aggagttatg cctagcagct
     4441 ctccggatgt ctatatcgag gagcacaatg tggtcattgc tccaggcggc ctggcggcca
     4501 gagcttggcc ggtgaattgg agcgccatct gggtcggcgc tttggcgtcg cttgccgttg
     4561 cgctcatctt cgggctcatc ggcaccgcca tcggcgctca ccaggtcggc aagaaggtgg
     4621 taaatctcca cgacctgacg ctgggcggtc tgattttcag cgtcgccggg gcgttctttg
     4681 cattcgtggt cggtggctgg gttgcgtcat caatcgccgg cctgcgccgg gccgaggatg
     4741 ccgccttgca tggcaccatc gcctggctcg cggcccttcc gctgttcctg tttctggcgg
     4801 ccctcggagc agggagcttc ttcgggggat ggtatggcgg gctggcggga acgccggtct
     4861 gggccacttc atcgacggtc acggcggatc ccaatgcagc gatggtcgcc cgcaactcgg
     4921 ccatcggcgc cgtgacggcg ctgttgatcg gcctggtggg gagtctcatc ggtggctggt
     4981 tggcgtcggg ggaaccgatg tcgttgacct atcgccggat gaaggaagac gacgacgtgc
     5041 ccggtcgtcg ccgtgcacgt gctgcgtaag aatggatcag gaaacgaact cagtagagga
     5101 ggtaccgtat ggcatacgtg attatgtggt tattgggtct gccgttgagc ttgatcgtcc
     5161 tgctgtggct cgtcggaatc ggaagatagc gggcgtaagg actgtcggtc tcggaacgag
     5221 cgagcttgga gggaccattg gtaaagtggt cccccaagca cacagccgta ggccctcctc
     5281 tcttcactct ctcatcgcta gacattcctc atcttcccgc gtaagttccc gctagtggct
     5341 cccaagcatt tctccatgat ccgcgagttt cacctggccg atgtgctcac gttggccaac
     5401 gccgcgtgcg ggcttgccgc cgtgctctgc gcgatgctgt acatgggcag tgagttaccc
     5461 gctcattatt tcatcgctgc ggcgctgacg cccgccgcgc ttgtcttcga ctggctcgac
     5521 ggccgggtgg cccgcttcag acatacccat tcggcgctcg ggcgggagct ggattcgctt
     5581 gccgacatca tctcgttcgg cgtcgcgccg gcgacgctcg gtttcgccgc cggcctgcgg
     5641 ggagcctggg actggctggc gctgatctat ttcgtctgct gcggcgtgag ccggcttgca
     5701 cgctacaacg tcaccgccga acgcctgtcg gaaggtagcg acaaggtggc ctacttcgaa
     5761 ggcacgccga ttcccaccac cattctcttg actgccgtgc ttgccgtcgc cggctggcaa
     5821 gaccgcatcg gggagcgtct ctattgggga tcctggtcgc ttggtccctg ggagcttcat
     5881 ccgttgtcat tgctcttcgt gatttccggc agcctcatga tcagcaagac cttgcgcatt
     5941 ccgaaaattt gatcgcggca caagtcccgg cgaggcgatt ctttttcacc gtctcgcacc
     6001 aacggcagga tgatttttgt gagtggacat tgtatcgtgc aggaaaacga ctgagcgaga
     6061 gcgccgtcac actggagggg ggattcgatg acacgagcat ggatcgcact ggcagtatgc
     6121 ctgtctctgg gcttagcagg ctgtggagga gaaaaggccg agattaaaga agatcggctg
     6181 acggtgggaa aggtgcaggg agaaatcaag gtcggcatgc cggcctcgca ggtggccgag
     6241 ttgctgggca gcccgaatat tgtaacgacg gacgataagc gtcgggaagt ctggatctac
     6301 gataaagtgt cgactgatcg ggtggatact gcaagttcct cttttggagg catcatcatt
     6361 ttgggcggga gttcgagcga cagatcgagt tcacagcgtc agcggaccct cactatcatc
     6421 atcaagtacg acgaagagaa gaaggtccgc gattttgcgt acaactcgac gcagttctaa
     6481 ccgggactct cgccgcgtga tgtctggatg ggccccggtc gcgaccgcct tgctcttctc
     6541 cagccagctc gccggctgca cgaccccgtc cgacccgagt gagctgtttc aactcacacc
     6601 ggaaagcgcc ataaaccgcg cgatgcagac ccgcctgttc gaaacgagtg atcagacaga
     6661 gttgctatcg gcctccgcgg cggtccttca ggacctcggc tttcaggtgg aggaaagcgt
     6721 gcaggatgtc ggctttctgc gggccacgaa ggagcgaagc gccaggcaat atggtcagga
     6781 catcggccgt tttctgatct tcgtgctctc gctgggacat gtcattatac cggtggatct
     6841 ccaccagaaa attgccgccg ttcttgtcga gcgaccgatc aatccggatg ggacgcgtcg
     6901 cgaagtccgg atcgtgttct atcgggtcat ctggaaagga gatggccacg ccgatcgcca
     6961 atacgtgccg cccggcgaac aacatatgga aatgctgcgg gacgcggcgc tgtaccaaca
     7021 attctttgcc aagctctcga aggcagtatt cctggagcca ttcacactat aacgacataa
     7081 cgacgaggtg atgatgagac gagcctggtg gggaaccctg gctatcgcgg gcacgctgtt
     7141 cgtgcagggc tgtgaaacgc cgcagccgac ccaggacctg ctcgccccga ccgaagcgca
     7201 aatgaagatg cgaagcttcc agacccgcac cttcgacctc gaagatcgtc tcctggccat
     7261 gcgcgccgtc atcgcggcct tgcaggactt gggtttcatc atcgaacggg ccaacgaatc
     7321 tctgggcctt gtcactgcag cacgattcgc tgaaccgaac tattacgacg tggtgggcat
     7381 cacggttacc gtgcgccagg agacgaaaga acggatgatg atacgcgcca atgccatcca
     7441 caacaacaaa cccatcgaag acccgaaggt ctatcaaaat ttctttgcga ccctcgaacg
     7501 gtcgctgttt gtgaccaaga cttagtcatg gaggtcgggc cgaaggtgga tagaaaggcc
     7561 atcgggaccg gaggcgtgcg ccgcacggcc gtcatgttgg ccgttgttct ttggctgccg
     7621 ttaacaggct gtccgacacc gtacgaatgg agacatgaag gtcagtggga ttcgcgggat
     7681 caggtctggc tctctgaaca aagtcaggtg aaactgcgcg ctgcacagag tcgcgtgttc
     7741 gatacgaccg accgccgacg cacgatagag gcggtgatcg acacgatgca agaccttggc
     7801 tttatggtgg aagtgctgga cgaaaacctg ggcatcgtct ccgggaagcg cttcgagccg
     7861 atcgaaacca gttccttctt caacgatccg ttctatcacc tctatagcga tgagagcttg
     7921 ttggtcttca ccaagtcgta ccgaacgtgg gggccatttt ggcatcgaag cgacctggtt
     7981 cgcctgacga taaccgtgcg caagcgcaat gaagctcaac tcgtggtacg cgcgagcgcg
     8041 caatactacc tgaaggctgt cgaagatccg ttgccctatc aaagattctt ccgcacgctc
     8101 gaacaagcgc tttttcttca ggctaagccg cccgtggagt aggacaaggc gagcgggaag
     8161 ctgcatccct tgtcgtcgct cttcattctc tccggcagcc tcatgatcag caagaagttg
     8221 cgcattctga aaatctcatg tgcaagcgca cgtgcttgtc cgattcatgt gtaagagaag
     8281 gagtaaaaaa tgaaggagat tgaacagaga gcttcacacc ggctgaacta gagagatcgt
     8341 cagtattgcc gccttcttag cactaacccg atggattgta ctgtgataga agcgtctcgg
     8401 ggcgattcgt gaggaggtga accgattagt tgccgattga agaattcacc gctgtgccga
     8461 tcacctgagc gaccgccaca ggtttttgca cgcctttaag tttcagttcg cctaccgatt
     8521 ccatcgtgac gaggcccttc acgtaagata ttacccgatc ggaaattaag atctgaccat
     8581 gttgtgcctc gctgcataac cgagctgcta agttggtgac ggggccgatc gcagcgtagt
     8641 ccttgcgccc ttcaaatcca atggcgccga tcgtagcata gccagtcgca atgcctattc
     8701 ccgctcccaa atcaataccg tgcgacttcc attcatgtcg caatgactct acggcctgcc
     8761 gcatcgcaac ggccagcttc acggcttgct cggcatgatt gggacatggc aatggatcat
     8821 tgaaatagac catgacagca tcgcccacaa agcgttctaa cgtcccgcca tattccgaaa
     8881 ccaggcgacc catgaccgcc tggtactcgc gaaggactgt catgacttcc tcgggtgctg
     8941 ctagttccac aaacgccgtg aatccccgaa gatcgataaa caatactgag atttcttgtc
     9001 gatgtcccct taagaaatca tcatgttcac ccgacatgat caagtcggcc aagttgggag
     9061 gaagaaatct cgaaatcttt tccttttgca attgcacaag ttcacgttct tgtgcgcggg
     9121 tacgaataag gttgcccacg cgtgccagga gttcaagctc gtcaaatggc tttgagaggt
     9181 agtcatcggc tcctgcgtcc aagctttcga cacgggcttc ggttccggca cgggcggtta
     9241 agaagattat ggggaccgat cgaagggctt tatgatgtcg taccgcctgc aacagatcat
     9301 gcccactcat tttcggcatc atgacgtccg tcacgattaa ttcaggatgt tggcgttgag
     9361 cctgttcaaa cgcttcagcg ccatctttcg cgagtacgac acggtagtcc tttcggagga
     9421 tccgttccac ataactgcgc atgtcggcat tgtcatcaac gaccaacacc aatggggcgt
     9481 tgagctttaa tgtaggggga gtatgagtgt caacctgtct ttcaatcggt tgaaggggaa
     9541 gaaaggtttg atcgtcctct tcatccgaaa cgccaaggac atggtcttta ttcacatttc
     9601 cacgaggtaa tgtgatggta aatatagacc cccgccctaa gtcgctctcc acggcgatcg
     9661 ttccgccatg cagcgcgaca atctctttga ccaacgccaa cccgatacca cttccttcgt
     9721 accgtctcgt cgcacctcct tcgacttgtg tgaagcggtc gaagatgcgt gccaactgtt
     9781 ggcgtggaat cccgatgcca ttgtcttcaa cctccaactc gatggattga tctttggagg
     9841 caaggcggat ccatacctgt ccggtttgcg gatcactgaa cttgaaggcg ttcgaaagca
     9901 ggttgtataa gaccttcttc aattgacgag agtcgccttc caacgccaca ggctctgtca
     9961 accccttcag gtgaattcgg cgtatagggc tggaatcaaa gtttgtgacc acacctcgaa
    10021 ccagtgcagc aaaatctaga gaccgtttcc gcagggccgc ccgtccactg tcaaatttgg
    10081 ccagatccag caactcattg atgaggaaaa gcagtcttga ggcattccgt accccgcctt
    10141 tgacgaggtc attggcttcc tcgctcagct tcgtcttcaa cagggtttga aacgccccca
    10201 gtgtgagcgt gaggggagtg cgtatctcat ggctgatgtt ggcgaagaaa tcacttttca
    10261 agcgatcgag ttcaagaagt ttctggttgg agatctccaa ctcctgccga gcatggcgct
    10321 cggatactcg ggcgaaaaat tctgcacgct ggagccgctc atagagtccg acggaaaaca
    10381 acaatgcgac acaggtccaa atgagaaaga aagaattttc tatggcagcg ttaaaatctg
    10441 gctgcgggcc aagggagttg ctcacataat aagatgacag ggtgacaagc tgtgccgcga
    10501 gatgactggg ccacgacacc ggaacaatca cggcgccggt caaatacacc aggttcagcc
    10561 cgttgtaata ctgcgaggag aagccgccaa gaattaccgt catctgggtg acacacaaat
    10621 tagccactac gacccacagg atgacgaacc agttaggcgg gatagacttg ggtccaaact
    10681 taaaaaggag aaatccgccg agcagcccga gttggaggac gacacgaaca atgaaaagcg
    10741 ggatcaggta gtcccggtgt tgcagataat ccaatgcgat gaacgcggga tttgcaataa
    10801 accctagata atagagtacg tgtaatcgct ccctgatgaa atttcttcgc caatcgtcgt
    10861 aggcctccgc tgcctcgcgc ccagcgctgt gctccatggg aatctcagca tgaaacaatt
    10921 tgttccagcc gcaagcatct ttcactctcg tccaaagtga tcccatgtgc atcgccactc
    10981 aagaaaagac ccgttctagc tcacgaagca aaatagagct gtcggctcag aatcggtagc
    11041 tcagggtatt ggaaattaaa tagccgctta tggattcggt tgcgtttggc ccgaacggca
    11101 gcgagggatt ggtataggtc acactgttct tcggagcgta gaggccggtg aagtcgtaag
    11161 cccatctttc agtgaaccga aatccgatgc ctgcagagaa atggtgctca tttattaacg
    11221 gccatagagg gctcaaggtc tcctttggga ctggattatt actgtagttg tacccggctc
    11281 tcagcacaag ccggtccttg tctttccaag acgactcttg caagaccgaa tacgagaatc
    11341 caagggcgat gatgacctga tctttccaat taaacggaat atttaagttc acttgagccg
    11401 gagccaatgc gttgtttgga ttcgtccctg taattaccac agtattggtg gaggcccagt
    11461 tgagccagct tgtcgtcaat gccaccatga gatgctctgt cggtttggcg gccatcgaca
    11521 catcgacttg ctgaggccac tttatccctt ccacccgcgc gtgatagttc accgtcccta
    11581 aaccgaagga agagaagttc agattagcct ggccaccggt gtaattgaaa ctgactggcg
    11641 agctgtaggt cgctccgacc gtcagccaag gaagagcacg gtacatggca ccgattttta
    11701 ctgcaaacac cacatctgaa gggcaatcgc cgcccagagc accgaggccg ccgttgcgcg
    11761 cgcaactgtc tcggatgtta aatccagcga atccgctcgg aaggcccggt gagggggcca
    11821 cagaggttcc tgggaataag cgtagagaga catcggagta tccaacactc ggagcgattc
    11881 caaatgacaa cttatctgtg gcttcatagc ttaatccaat cgcaaacttg agatatcgaa
    11941 aaaaggagga cccggcatcc tgagttccaa atgccgttgt cagatttcga tagtccgacc
    12001 caaagcctcc ctgtgtgaaa attcccgctc caatcgtgag gccaggcacc gaggaaagtc
    12061 gtgtagcgac gccgagattt cctatggcgt acgcattgtt ttggccagcc acattattat
    12121 taccgaaggc atccgagtgg tgcagaaacg cctggataaa tcctgccgtg acatccaacc
    12181 gagtggcttg gatcagtgac attgcggccg gattggtatt gatggccgaa gtgtctgcca
    12241 ctgcgacatg tccagatccg gccagcgcag tcgattcggc ggagaaaccg atgaattgag
    12301 gaccgttaag agctagagcg gttgttgtgc tgaagagcgg cgagctaaga gcgacgagga
    12361 gcagtgagca tcgcgagagc ttccttgttg gtaccatcta atgttccgcg ttgctcgatc
    12421 aactgccggg tttcgcggat atagaccatg gctttcattg agaaagccat aacactctcg
    12481 gaacgaagcc agattctaga agactcgcgt aagacgcgca atagggctag atagaagacc
    12541 aaaaaaatac gtgctattta agattagtga gctagcctgt agacgtatca gcagacactt
    12601 gcgggtgaag aaatctagac aagcaacgca taccaccttg tccatgcctg agtgttttaa
    12661 ggcgggggcc gaaggggcaa atagatgtga aaagttgtcc ctcttcccag ttcactctcg
    12721 acagtgattt tgcccttatg ggcatcgacc acgtctttga cgatttttgt tcctaagccg
    12781 gtcccgccgc gtttgctact cctagcagct ggggtaaata gggcatcgcg tatgtcttga
    12841 ggcataccct ttccagtatc tgttaccgtg agatgaactg ataccccaac tggttcttcc
    12901 ttcgcggcta tagtgattgt tcctcctgcg ggaacttcag gaatagcatt gttaatcaag
    12961 ttatatagtg cattgaacag ccgtcgctcg tccgccacga actcggctgt gagatcaagc
    13021 actgaggtgc aaatcgagac ccctttctga tcggccgacc atttgagcgt gtgcaacacc
    13081 tcctcgacga ctctattcag ctgacaaggc ttaaattccg gcggtgtgct gagtcccttg
    13141 acgcaatcag caatctcctt tacacggtct tgaatccgcc gcgtagaatt gtccaccatc
    13201 tgaatgacgt cttggcatcg atcgaaacgt tcctttgctt gcttgtcttc atgcttgaga
    13261 gcgtctccca gcaagtcatg gagatcgctc tgcaataaat cggtgccaca taccacgggc
    13321 attagtaagt ttttaatatc gtgaccaatg tcgccgagta accgagctac ttccgccaat
    13381 ttcgcttctt gaaatagacg agcctgctca atggacgatg ccgtgatcgc agaaacaatg
    13441 gtcaaaatgg cgagatcctc tccgttgagc agaccttccc gcttgttcat gacctggagc
    13501 acgccaatcg ggtcgccttc ccaacgcttc aaggggaggg tgatgagatc tcgggtgtgg
    13561 tagccggtca attgatcgat ggactccaaa tgacgcggat ctttctgtgc atcggcaata
    13621 acgataggtt ctcccgattg aaaaacggtc ccggcgagtc cttcattcca ggaaatggcg
    13681 gtgccggacg gaacggaaga ggcgccgata gaatggtgaa agatcaactg ttgggatggt
    13741 gggtcggcca acaaaatgga gccacattct gcgttggcca cctcaatggc aatctggagt
    13801 gccttaacgg ccaattcatc cagcgcaaga tgctcgaaca atgcgctcgt tattcgtact
    13861 gcggcctgaa actcacgttc gcagcgcagt aatcgtttat ctctataccg tcgctcactt
    13921 cgtcttcgct cttctttcgg ttcaatgaac atggcgccaa cctatgtatc ctcatcacgt
    13981 tggcccgacc gttaacataa ttctcccaga aagtacccga agatagcatg tttagaagtc
    14041 acaattcgac aaggcgcggt cttgggacat cctgagggaa gaaaacggat cgtggaatgt
    14101 gtagaggcga tgccatgacc attatgaaca agtcagattg atatcggtta ggagtaagat
    14161 ttttttattc gcctaagatg tcggtggagg tttcc
//
LOCUS       DASSCQ010000062         4969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_500759, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000062 DASSCQ010000000
VERSION     DASSCQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500759"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFH05_04410"
     CDS             <1..311
                     /locus_tag="VFH05_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET6677485.1"
                     /translation="DGKSLSQRGTGDGPVDAVYRTIAAITQTKSTLLMYVVKAITGGT
                     DAQGEVSVRVQEDGRTVSGHGADTDIITASARAYLNALNKLAYLATKQAQGEQKVNLI
                     "
     gene            372..734
                     /locus_tag="VFH05_04415"
     CDS             372..734
                     /locus_tag="VFH05_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6677486.1"
                     /translation="MFKVTPADRPEFLAGDHTRLREVFHPAKHPLKLGYSLAHGALGP
                     GQRSKWHVLNSSEVYYFLTGHGRFMIDEQVTTVEAGTTLYVPPGSKQSLENMGTDTIE
                     FLCVVDPAWKTEDERILE"
     gene            802..1875
                     /gene="leuB"
                     /locus_tag="VFH05_04420"
     CDS             802..1875
                     /gene="leuB"
                     /locus_tag="VFH05_04420"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET6677487.1"
                     /translation="MKAKIAVLAGDGVGREIVPEAVKVLRAVGERCGHHFEFTNGDIG
                     GQAIDKVGVPLPQDTLTLSKQSDAVLLGAVGGPKWEGLDYSLRPERALLGIREALGLY
                     ANLRPAKLYPNLTDASTLKKEVVEGIDILVVRELTGGIYFGKPKGIEKLPNGSERGVN
                     TEVYTTEEIRRIAMVAFDAARKRRKKLTSVDKANVLESSELWRRVVVDVHKDYPDVSL
                     SHMYVDNAAMQLVRNPRQFDVMLCNNMFGDILSDEAAMLTGSIGMLPSASLGAQVGLF
                     EPIHGSAPDIAGKNIANPIATIASAAMMLSYAFKMEKEAELIEQAIVKALDLGYRTKD
                     IQSPGTRVVGTTEMGDVIMRSLG"
     gene            1905..3077
                     /locus_tag="VFH05_04425"
     CDS             1905..3077
                     /locus_tag="VFH05_04425"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677488.1"
                     /translation="MIRTVAGSGEAGWSGDGGAAVAACLNEPKAVALDHDGNLLIADS
                     ENHVIRKVDRRSGLITTVAGCTSSHDSPLTVMNRAAVPPTDDPLSEGSSSAEAAFVQQ
                     SDLSGTVRYVVNGVGSTKRFAGDGGLALDALLNFPTAIAADAEGDLYIADTMNHRIRR
                     VDARTGRITTIAGVGLPRFGGDGGPSVAAGLNEPAALVVQGGRLYVADQSNNRIRMVD
                     LRTGLITTVAGTGTATFNGDGRPATEAALAGPSGLTIGTDGRLYIADTFNGRIRAVDP
                     ATGIIETVVGDGGEYRYQGEMEAASPSLSRPSGIAVDVEGNVFMTDSDNHLVRRWDRV
                     AGRIDRVAGTGNANYGGDGKTPLEASLNYPFGLVIDQENHLFVADTFNHRIREIVL"
     gene            3182..4204
                     /locus_tag="VFH05_04430"
     CDS             3182..4204
                     /locus_tag="VFH05_04430"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET6677489.1"
                     /translation="MLKKQSSYVIAILGATGAVGHETLDILDERKFPIAGLRLFASKR
                     SAGGVLTCQGKEWTVEELTPDVSFAGVDIALISATDAISREYGSRLGAAGVMVIDDSA
                     VFRMDLDVPLVVPEVNAQALGSMPRGIVAIPNCTTTPLVMALKPLRDAVGLKRVVVTT
                     FQSVSGTGAAAMEELVTQTKDLMAFRDVKTEVYPYQIAFNLLPHIGSFSEEGDCSEEV
                     KIANETRRILDAPTLRVTATTVRVPVLRCHSEAINVELEKPLTANEARAALAAMPGVI
                     VYDDPSRKLYPMPLDATGKDEVYIGRIREDLSVTNGLNLWVVSDNLRKGAALNAVQIA
                     ECLVKG"
     gene            4204..4458
                     /locus_tag="VFH05_04435"
     CDS             4204..4458
                     /locus_tag="VFH05_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET6677490.1"
                     /translation="MIEWESVGKILIGVGTVVALLGVLFLLVDRVPTLSHLVGWLGRL
                     PGDISIKRDNFSLYFPLATSIVLSVILSLLFYVLAWIFRR"
     gene            4440..>4969
                     /locus_tag="VFH05_04440"
     CDS             4440..>4969
                     /locus_tag="VFH05_04440"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET6677491.1"
                     /translation="MDLPPITLPSRLSLCLVMVVSVVLASHAALAAQSIRVLLSADVY
                     RLEVQADGAVWLTDSSRDGRAIRSDARITTYGKGFLVNGTRIAHERLTLRAGERGLTL
                     MLVKPNDKLPSVSKPVKPVNDQRLAIPVSGLVHVVRKGKGFLVINQVDLEEYVKGVVP
                     GEVNSSWHPEMLKAQAV"
BASE COUNT         1064 a         1342 c         1565 g          998 t
ORIGIN      
        1 tcgacggcaa gtctctcagc caaaggggga ccggcgatgg ccccgtcgat gctgtctacc
       61 ggacaattgc cgccattacc cagacgaaaa gcacgctgct catgtatgtc gtcaaggcga
      121 tcaccggagg gaccgatgcc caaggcgaag tctccgtcag agttcaagag gatggacgaa
      181 ccgtgtcggg ccatggcgcg gacactgaca ttattacggc gtcggcaagg gcgtacctga
      241 atgctttgaa caagctggcg tatttggcca caaaacaggc gcaaggagag cagaaggtaa
      301 acttgatttg atgaaacctt gcacggtaca gtgccgtctt caccaagctg ataatggcga
      361 agggggctcc tgtgttcaag gtcacgccgg cggatcggcc cgagttcctc gccggcgacc
      421 atacgcggct tcgcgaagtg ttccatccgg ccaagcatcc gctgaaactt ggatatagcc
      481 tcgcacacgg ggctctaggg cccggtcagc gttcaaagtg gcacgtcctg aattcatccg
      541 aagtctatta tttcctcacc gggcatggac ggttcatgat tgatgagcag gtgacgaccg
      601 tggaagccgg cacgaccctc tatgtcccgc ccggcagtaa acaatcgctt gagaatatgg
      661 gaactgacac catcgagttt ctctgcgtcg tcgaccccgc ttggaaaact gaagacgagc
      721 gaatattgga ataacgccac ctggggtagg cctcgggggt gtcggaagtg tcacgaagcg
      781 gaagctaacg gcggaggcag cgtgaaagcc aaaatcgcgg tattagcagg cgacggggtc
      841 ggtcgggaga ttgttccaga agcggtcaag gtgttgaggg cggttggtga acgatgcggg
      901 catcactttg agtttacgaa cggcgacatc ggcggtcagg ccatcgataa agttggagtg
      961 cctctacctc aagacacgct gacgctgtcc aaacaaagcg acgccgtgct gctgggggcg
     1021 gtcggaggac cgaaatggga gggattagat tacagtctca gacccgagcg ggcgttgttg
     1081 ggcatccgtg aagcgctcgg tctctatgcc aatctgcgac ccgccaaact gtatcccaac
     1141 ctcaccgacg cctccacatt aaaaaaagag gtcgtggaag gcattgatat cctcgtcgtc
     1201 cgagaattga ccggcggaat ctatttcggc aagccgaagg ggattgagaa gctgcccaac
     1261 ggcagcgagc ggggagtcaa taccgaagtg tatacaacgg aagaaatccg tcgcatcgcc
     1321 atggtcgcgt tcgacgccgc tcgaaagcgc cggaagaagc tgacctcggt agacaaggcc
     1381 aatgtgctgg aatcctccga gctatggcgt cgtgtcgtgg tggacgtcca caaagattat
     1441 ccagacgtca gtctcagtca tatgtatgtt gacaatgcgg ccatgcaact tgtgcgcaat
     1501 cctcggcaat tcgatgtcat gctctgcaac aacatgttcg gtgacattct cagcgatgaa
     1561 gccgcgatgt tgacgggctc gatcggcatg ctgccgtccg ccagcctcgg agcgcaggtc
     1621 ggcctattcg agccgatcca tggcagcgcg ccagacatcg caggcaagaa catcgccaac
     1681 ccgattgcga cgattgcctc ggcagccatg atgctgtcat acgcattcaa aatggagaag
     1741 gaagccgagc tgattgagca ggcgatcgtc aaggccttgg atctgggtta tcggacgaaa
     1801 gacattcaga gtccgggtac cagggtggtc gggacgactg aaatgggcga cgtcatcatg
     1861 cggagcctcg gctagtgacc atgagcgttg atgcccgcac cggcatgatc agaactgtcg
     1921 ctggaagcgg cgaagccggc tggtccggcg acggcggagc cgctgtcgcc gcatgtctga
     1981 acgaaccaaa agccgtggcg cttgatcacg acggcaatct tctcatcgcc gattccgaaa
     2041 accacgtcat tcggaaagtc gatcgaagga gtggactcat caccacggtg gcaggatgta
     2101 cgtcgtcgca cgactcgccc ttgacggtca tgaacagagc ggccgttccc ccgacggatg
     2161 acccattgag cgaaggcagt tcgtctgcgg aagctgcctt cgtgcagcag tcggacttga
     2221 gcgggacggt ccgctatgtg gtcaacggag tggggagcac gaagcgattc gccggagacg
     2281 gcgggcttgc gctcgatgcg ctcttgaatt ttccgaccgc gattgcggcc gatgctgaag
     2341 gtgatttgta tattgccgac acgatgaatc atcgaattcg tcgcgtggac gcccgtaccg
     2401 gccggatcac gacgatcgcc ggcgtgggcc ttccccgatt cggaggtgat ggagggccgt
     2461 ctgtcgccgc tggactcaac gagccggctg cgttagtggt gcagggaggc cggttgtacg
     2521 tcgccgatca gagcaacaat cgcatccgca tggtcgattt acggacggga ctgatcacga
     2581 cggtcgccgg gacaggaacc gccacattca atggagatgg cagacctgcg acggaggccg
     2641 cgcttgccgg accgagcgga ttgaccattg ggacagacgg caggctctac attgccgata
     2701 cgtttaatgg aagaattcgg gctgtcgatc cggcgaccgg cataatcgag acagtggtcg
     2761 gtgacggcgg cgaatatcgg tatcaaggcg agatggaagc tgcttcgccc agtctgtctc
     2821 ggccatccgg gattgcggtt gacgtggagg gcaatgtgtt catgactgat tccgacaacc
     2881 atttggttcg acgatgggat cgagtggctg gtcggatcga tcgagtggcc ggcacgggca
     2941 acgccaatta tggtggagac ggtaaaacgc cgttagaggc cagtctcaat tacccattcg
     3001 gcctcgtgat agaccaagag aaccatctct tcgtggcgga tacattcaat catcggattc
     3061 gagaaatcgt gctgtaaccg gacgctatgt tggcagccgg ccgactgttt gctggcagcc
     3121 gaggggtgtt cgttgagtcg gtggaacggc gcacgttgca acggttagga acgcctggtc
     3181 catgctaaaa aagcaatcct cctatgtcat cgcgatcctc ggcgcgaccg gcgcggtcgg
     3241 ccatgagacg ctcgatattc ttgacgagcg caaattcccc atcgccggcc ttcgtttgtt
     3301 cgcttcgaag cgttcagccg gaggggtgtt gacttgtcag ggaaaggaat ggacggtcga
     3361 agagttgacg ccggacgtgt ccttcgccgg cgtcgatatt gccttgatct ccgcgacgga
     3421 cgccatcagc cgggagtacg gcagccgttt aggggcagca ggggtcatgg tcatcgacga
     3481 cagcgcggtg ttccgtatgg acctggatgt gccgttggtc gtaccggagg tgaacgcgca
     3541 ggccctcggt tccatgcctc gcggcatcgt ggcgattccc aactgcacca cgacgccttt
     3601 ggtcatggcg ctcaagccgc tgcgagacgc ggtcggcctg aaacgggtcg tggtgaccac
     3661 ttttcagtcc gtttcgggga ctggcgcggc ggcgatggag gaactggtca cgcaaactaa
     3721 ggatttgatg gcctttcggg atgtgaagac ggaggtgtac ccctatcaaa ttgccttcaa
     3781 tttgttgcct catatcggct cctttagcga ggagggtgac tgctcggagg aagtgaagat
     3841 cgccaacgaa accaggagga ttctggatgc accgaccttg cgggtcacgg ccaccaccgt
     3901 ccgggtacca gtgctgcgat gtcactcgga ggccatcaat gtggaactgg aaaagccgtt
     3961 gacggccaac gaggcccggg cagccttggc tgcgatgccg ggggtcatcg tatacgatga
     4021 cccaagccgg aagctctatc cgatgccgct tgacgccacg ggcaaagatg aggtgtacat
     4081 cggacgcatc agagaggacc tctccgtcac gaatggcctg aatctgtggg tggtatccga
     4141 caaccttcga aaaggcgccg cgctcaatgc cgtgcagatc gcggaatgtc tggtgaaagg
     4201 ctgatgattg agtgggaaag cgtgggcaaa atcctcattg gtgtcggaac ggtggttgcc
     4261 ctcttgggag tcctgttcct gctcgtcgac cgggtgccga ccctcagtca tctggtgggc
     4321 tggctcggaa ggcttcctgg cgatatttct attaaacggg acaactttag cctctatttt
     4381 ccgctcgcga ccagtatcgt cctcagcgtc atcctcagtc tgctattcta tgtactagca
     4441 tggatcttcc gccgataaca ctgcccagcc gtctcagcct gtgtctcgtc atggtcgtct
     4501 ctgtggtgct ggcatcccat gcggcacttg cagcccaatc gatccgcgtg ctgctctcgg
     4561 ccgatgtgta ccggttggag gtccaagccg acggcgctgt atggctgacg gactcaagcc
     4621 gcgacgggcg agcgattcgg tcggatgccc gcattacgac atacggcaag ggatttctcg
     4681 tcaacggcac ccggatcgcc cacgaacgct tgaccctgcg ggcaggagaa cgggggctga
     4741 ccttgatgtt ggtgaagccg aacgacaagc tgccctcggt gtcaaagccg gtcaagccgg
     4801 tgaacgatca acgcctggcc atcccggtga gcgggttggt ccacgtcgta cgaaaaggca
     4861 agggcttttt ggtcatcaat caggtggacc tcgaggaata tgtcaaagga gtggtccccg
     4921 gggaagtcaa ttcttcctgg cacccggaga tgttgaaagc acaggccgt
//
LOCUS       DASSCQ010000063         7507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1703946, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000063 DASSCQ010000000
VERSION     DASSCQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703946"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..618
                     /locus_tag="VFH05_04445"
     CDS             115..618
                     /locus_tag="VFH05_04445"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HET6677492.1"
                     /translation="MNIETNAVVGAIAIGIGATLFMDLWNLLLKRAFSILSLNYCLLG
                     RWLRHMPEGRFRHINITAAPPKPYECTVGWIAHYTIGVVLAVAFVLASGEWLTRPTLL
                     PALLYGVGTVVFPFFLMQPSLGLGIAASRTPNPTQARLKSFATHIVFGVGLYLCALGV
                     SHLLLFT"
     gene            776..1570
                     /locus_tag="VFH05_04450"
     CDS             776..1570
                     /locus_tag="VFH05_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6677493.1"
                     /translation="MPVLQINGIGLHYEESGNRDHPTIVFAHSLIWGASVFTELLTEI
                     AKDARLLTVDLHGHGLSGYREGMTLDEMAEDCYGLLKTLMVGKITWLGCGIGGMIGMR
                     LALAHPDALDSLVLIATSARPDPSTTKASALHLWKMFRDGHRQDIADSAMKLFFAPRT
                     YQSRPELIANCRQELVRTSDARGMFAAALAAFNRSDLSSELHRIKARTLVLAGREDPA
                     ATPAQADFIASQIPGAQFKIVDEASQLAAIEKPLEVARLVREFLSE"
     gene            1887..2285
                     /locus_tag="VFH05_04455"
     CDS             1887..2285
                     /locus_tag="VFH05_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6677494.1"
                     /translation="MDVCWEEAVRRGDAEVMRDLLSRGIDPNARDPHGQTGLMLAAHA
                     GHLAAVQLLVDHGVDLNVTAKFGLSAVMLAVTAGHKEIAQVLARAGADLRLRGTGAPG
                     FAGKTAADLAGARGWNDLAIALAPDKPGMT"
     gene            2282..3109
                     /locus_tag="VFH05_04460"
     CDS             2282..3109
                     /locus_tag="VFH05_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677495.1"
                     /translation="MTPPVPPRIYCITAPEAPTVAVFRRGPTNWSHVGQWDLAGLRYE
                     PGAWLGGRIFPRRCDLSPDGRFLSYFAHKPSATWELGEAYVAISKLPWLTALHAFSTC
                     GTWTRGYCFTKDPSTSADEVPDLPIPYRLQSIPAVQFATELRRGWAEAPDSPPRDPRD
                     VWDQRRNARIQKRQPGGHRVLCVESTGHAGGEFGTEQAIDGLRVQYSLEADGQLTLLN
                     DLQWADWDGEGRLLVATRTGRLQIRHFASKREHTVFEEDLALLDPKPVPAPDWARHW"
     gene            3242..4123
                     /locus_tag="VFH05_04465"
     CDS             3242..4123
                     /locus_tag="VFH05_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677496.1"
                     /translation="MMSVKHPLPTASFKRLAILCALLLGFPTVAISASDEQTVGELME
                     LSGLTQQIPHLPNHYMTLVDQLLVGLERQRHPVSKTLRSQIRQSFAESLTPENFASEI
                     RSRLIENLSEDAALATTSWLRSDLGRKITTAEVNASSAEQSVQFATFFLQLQLERPAP
                     ERLQVVRRIEAITQGSEMATEAWEAIVAAIGRALEGEYRAKNPQNKTNLEEYLASMRG
                     SVKGMFEQGRLFQGLFTYRAVTDEELKEYAEFLESPAGKDVTHTINRAVQGAAIDAIQ
                     SIKPSRTKSIQPPRETA"
     gene            complement(4211..4735)
                     /locus_tag="VFH05_04470"
     CDS             complement(4211..4735)
                     /locus_tag="VFH05_04470"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6677497.1"
                     /translation="MPDQLTAERIIELLGLKPHPTCGFVTETYRAQQRIPDEALPTVY
                     AGSRPFASVLYFMVTADARIRLHRIRSDQMYHYYIGEPLEVLLLYPDGTGEIKILGAD
                     LNGGMRPQLLIPGGTYHTSRLGKGSAATAFSLLGTTEWPGFEPPDLELAEPSTVIAAY
                     PALRREIEEFTNTR"
     gene            complement(4817..5254)
                     /locus_tag="VFH05_04475"
     CDS             complement(4817..5254)
                     /locus_tag="VFH05_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6677498.1"
                     /translation="MILHIDHVTVVVRDVQKAKAFFALLGFVEEKSTVISGSTFARYM
                     GVEGIEAEHVTLVSKQSTPRFEIQLLRYIKPAHLPDADVANLAKLGYNHICFAVTDLD
                     AEVERLTNQGVPLRTEVLDFHARKLVFLSGPDGITVELAEWHA"
     gene            5460..5894
                     /locus_tag="VFH05_04480"
     CDS             5460..5894
                     /locus_tag="VFH05_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HET6677499.1"
                     /translation="MPQAEELFKTLGGATIFKRQVHSAEDVRAIIHKGFPYQSLEFVL
                     SKFHLDLPRMSRILSVPPRTMARRKAEQRLTAQESDRLARVARILTYATNVFGTEEKA
                     STWLTRPHRILEGTAPIELLDTDLGTQAVEAMLGRLEHGVIG"
     gene            5894..6349
                     /locus_tag="VFH05_04485"
     CDS             5894..6349
                     /locus_tag="VFH05_04485"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HET6677500.1"
                     /translation="MVVWRLTLKKHSAPDGEGARRYGGRWNTPGTPVVYTSGTLSLAA
                     LEYLVHVDSDILPDGLVSIRATIPDGLKIEAISASHLPLEWKDKIIPVSVQQIGTDWV
                     TNAKSAVLKVPSVVIEHEWNYVLNPRHPEFSQITWDAAAVFSFDQRLLK"
     gene            complement(6414..6983)
                     /locus_tag="VFH05_04490"
     CDS             complement(6414..6983)
                     /locus_tag="VFH05_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HET6677501.1"
                     /translation="MSMMRQSHNCMLRLGLLVLVGITIVASNGHAGSAPDSNPTAHSS
                     EQNWGKKLPSASRFTVLSLFEDAAVRDDETGLVWEKTVETTEMSWTDARAVCADKNVG
                     GRKGWRLPSISELASLVDPSTRSGPTLPAGHPFTNVLMDVYWSATSVTDNPKNAWLVF
                     FDTGKTTFAFKTITFHVWCVRGTMPAGQY"
     gene            complement(7289..>7507)
                     /locus_tag="VFH05_04495"
     CDS             complement(7289..>7507)
                     /locus_tag="VFH05_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6677502.1"
                     /translation="PSNKELSDARANSAAQALRDGGVRANLEAAGYGDSNPIAANTTA
                     TGRAKNRRVEIIVASAANQAVAKTPDLP"
BASE COUNT         1643 a         2080 c         2137 g         1647 t
ORIGIN      
        1 caacgacagc tgacctgggc ataatgcatt ggctgtgtgt cgggaaacgg ttcaagaaac
       61 tgctctccca ggcccgacgc cgaattaggc tgagtgagtg aactgtgaga ggtcatgaac
      121 atcgagacaa acgctgtggt aggagccatt gccattggta tcggagcgac tctgttcatg
      181 gacctgtgga atcttctcct gaagcgtgcg ttcagcattc tctcgctcaa ctactgcctc
      241 ctcgggcgtt ggcttcgcca tatgcccgag ggcaggtttc ggcatatcaa tattaccgcc
      301 gcacctccaa aaccatatga gtgcacggtc gggtggatcg cccactacac aatcggagtc
      361 gtgttggcgg ttgcgttcgt cctggcatca ggggagtggc ttacccgtcc caccttgttg
      421 cccgcgctgc tctatggcgt cggcactgtc gtgtttccat ttttcctcat gcagccgtcg
      481 ctcggactcg gcatcgcagc ctcgcgaaca cccaatccca ctcaagccag gctaaaaagc
      541 ttcgcgacac atatcgtctt cggagttgga ctgtatctct gtgcactcgg agtcagccat
      601 cttttgctgt tcacctgaga tcgttgcccc caacgacgct gtcccctttt cttttcccca
      661 gaatggtcca cgtttccctc ctttatctga tgtcttgaat caagagactc tttcccagta
      721 gtgtcgtgcg cgagcacgta aaagttgttg cctgccacta gagggagaga agcgtatgcc
      781 ggttctgcaa atcaacggga tcgggctcca ttatgaagaa tcggggaacc gggaccatcc
      841 gacgatcgtg ttcgcccatt cactgatctg gggcgcaagc gttttcaccg agctgctgac
      901 ggaaattgcg aaagatgccc gcctcctgac ggtcgatctt catggccatg gcctaagtgg
      961 ataccgcgag ggcatgacgc tcgacgagat ggcggaagat tgttatgggc tgttgaaaac
     1021 actgatggtt gggaagatta cctggttggg atgcgggatc ggtggaatga tcggcatgcg
     1081 tctggcgctt gcacatccgg acgctcttga ttcgctcgtg ctcatcgcga cgagtgcccg
     1141 acctgatccc tcaacgacga aagcgtcggc gctccacctg tggaagatgt tccgggacgg
     1201 acaccggcag gacatcgccg actcggccat gaaactgttc ttcgcgcccc ggacgtatca
     1261 atcccggcct gaactgattg cgaactgcag acaggaactc gtgaggacga gtgatgcccg
     1321 aggcatgttt gccgcggcgc tggctgcttt caatcgatca gacttgagca gcgagctcca
     1381 taggattaaa gctcggacgc tggtccttgc cggtcgggaa gatccggcag ccacgccggc
     1441 acaagcggac tttatcgcct cacaaattcc cggtgcgcag ttcaaaattg ttgatgaggc
     1501 cagccagctc gctgcgatcg aaaaaccctt ggaggtcgcc cgactggttc gagagttcct
     1561 gagtgaatga ctgagggaag cacacagtcg aaaggaagga gggaggtctg tatggccgta
     1621 acattgatta atgtattttc ggtccccgta gggaaggaag atgaattcgt cgcgtgggtg
     1681 gcatgacgca gcttaggagc gaaatccaac cagcaggggt ctcagaatca caagcaaatc
     1741 gcggcttcac acgttatttc ccggttggta acacccggtg tgccagccag aacacatatg
     1801 tgccatacca gatcgtcgga aaccacgaga caacggcaaa ggtccatggt gaacacaagg
     1861 gtgtttgctt cccgggagtc ggttgaatgg atgtctgttg ggaggaggct gtccggcgtg
     1921 gcgatgccga ggtcatgcga gatctactct ctcgtggcat cgatcccaat gcgcgcgatc
     1981 cgcacggtca gacgggttta atgctggccg ctcacgcggg ccatctggcc gcggttcaac
     2041 tcttggtcga tcacggcgtc gacctcaacg tcacagccaa attcgggctc agcgcggtga
     2101 tgctggccgt gactgccggc cataaggaga tcgcacaggt actcgctcgc gccggtgcgg
     2161 atttgaggct acggggcacg ggggcgcctg gcttcgccgg aaagaccgcg gccgacttgg
     2221 ccggcgcgcg aggctggaac gacctcgcca ttgcactcgc tcctgataag ccggggatga
     2281 cgtgactcct cctgtgcctc cgcgaatcta ttgcatcacg gcgccggagg cacctaccgt
     2341 cgccgtcttt cgacgggggc cgacgaactg gtcgcatgtc gggcagtggg atcttgccgg
     2401 tcttcgctac gaacctggcg cgtggctcgg tggtcgtatc ttccctcgcc gttgcgatct
     2461 ctctccggac gggcggtttc tttcctattt tgcacataaa cccagtgcga cctgggaact
     2521 gggcgaggct tatgtcgcga tctccaagct cccctggctg acggcgcttc atgcgttttc
     2581 gacatgcgga acctggacgc gcgggtattg cttcacgaag gaccccagta cctctgccga
     2641 tgaagtgccg gacctgccga ttccgtatag gttgcagtcc atcccggccg tccagttcgc
     2701 aaccgagctg cggcgtggct gggcggaagc gccggacagt cctccacgtg atcctcgaga
     2761 cgtctgggac cagcgtcgca acgcgcgcat ccagaagcgc caaccgggtg gtcatcgagt
     2821 gctttgcgtc gagagcaccg gccatgcagg aggggaattc gggacggaac aggcgatcga
     2881 cggcctgcga gtgcaatatt ccctcgaggc agacggacaa ctcaccctct taaacgatct
     2941 acagtgggcg gattgggacg gagaagggcg attactggtt gcgacgcgaa ccggcagact
     3001 ccaaatacgg cattttgcct cgaagcgtga acacaccgtg tttgaggaag acttggctct
     3061 cctcgatccg aagcccgttc cggctccgga ttgggcccgt cactggtgag tcaccgagtt
     3121 gggtatggcg cggtcacgat ctcacggccg cgtcctcttt gcctcactct ttagaggaat
     3181 tgttctcctc gcgctctctt ccttatcgtg actcaacaac tcagataaag cgagtgacgc
     3241 catgatgtcc gtcaaacacc cactgccaac agcgtcgttc aagcggttgg ccatactgtg
     3301 tgctctgttg cttggtttcc cgaccgtagc catttcggcg tcagatgagc agaccgtggg
     3361 ggagctgatg gagttgtccg gtctgacgca gcagattcca catttgccga atcactacat
     3421 gactctcgtt gatcagctgc tggtggggtt agagcgacag cgtcacccgg tgtcaaaaac
     3481 tctgcgaagc cagattcgcc agagcttcgc ggagtcacta acacccgaga actttgcctc
     3541 agagattcgg tcacggctga tcgagaatct ctctgaggat gcggcccttg cgacgacgag
     3601 ctggctgcga tcggatttgg ggcgcaagat caccaccgct gaagtcaacg ccagttcagc
     3661 agaacagtcc gtccagttcg cgaccttctt tttgcaatta caattggaac gtcctgcgcc
     3721 ggagcgactc caagtcgtgc gacgcattga ggcgatcact caaggtagcg agatggcgac
     3781 tgaagcttgg gaagccattg tggccgctat tggtcgcgct ctcgaaggcg agtaccgggc
     3841 caagaatccg cagaataaaa cgaatctaga agaatatttg gcctccatgc gcggcagcgt
     3901 taagggcatg tttgagcagg gacgattatt tcaaggtctc ttcacctacc gggcggtgac
     3961 ggatgaagag ttgaaggagt atgcggaatt tctcgagagc ccagccggca aagatgtgac
     4021 ccacaccatc aatagggcgg ttcaaggtgc ggcgatcgat gccatccaga gtatcaaacc
     4081 ttctcgcacc aaaagcatac agccgccgcg cgaaacagca taaaccattg cacggccttc
     4141 cgtagcctcg acgatgaacg agcgaggttg tcatgccccc gccaatcggt ttctgattct
     4201 tctttaaaac ttaccgtgtg ttggtaaatt cctcgatctc acgacgaagg gcagggtagg
     4261 ctgcaatcac cgtcgacggc tcggctaatt cgagatctgg gggttcgaat cccggccatt
     4321 ccgttgtgcc aagcaaggag aaggctgtag ccgcactgcc cttgcccaaa cgcgacgtat
     4381 gatatgttcc gcccggaatc agcaattgcg gacgcatccc gccattcaaa tccgcaccga
     4441 ggattttgat ctctcccgtc ccgtccggat atagcaggag gacttccagc ggctcaccga
     4501 tatagtagtg gtacatttga tcggaccgga tgcgatggag gcggatccgc gcgtctgcgg
     4561 tcaccatgaa atagagcaca gacgcaaatg gccggcttcc cgcatacacc gtgggcagtg
     4621 cttcgtccgg gatgcgttgc tgcgctcggt acgtttccgt aacgaagcca caggtaggat
     4681 gcggcttcaa gccgagcagt tcgatgatcc gctctgccgt cagctggtct ggcataagcc
     4741 ggaagcatac tacaaagatg gaaggaaaat ccggctcgcc acgcgccgcg aacgggtctg
     4801 accctgtttc ttcttcttaa gcatgccatt cagccagttc cacggtaatt ccatccggac
     4861 cggagagaaa tacgagtttc ctggcatgga aatccaaaac ctccgttcgt agcgggacgc
     4921 cctggttcgt taaccgttcg acctccgcat ccaagtcggt gacggcgaaa cagatatggt
     4981 tgtagccgag tttggccagg ttcgcgacat ccgcatcggg caggtgtgcc ggcttaatgt
     5041 atcgcaggag ctggatttca aagcgaggcg tcgactgctt tgataccagc gtgacatgct
     5101 ccgcttcgat gccttctaca cccatgtacc gggcaaacgt cgatccggaa atcacagtcg
     5161 acttctcctc gacaaatcca agaagcgcaa aaaacgcttt cgccttctgg acgtcccgta
     5221 cgacgacggt gacatgatca atgtgtagta tcatgctgct ggaggagtaa actaaacctt
     5281 gagctcttat cgactaagat agcgtaggat aaaggttaac tgcaaaagta gcgagaaggg
     5341 agtggaattc acggggagtg atgcattgct gaacatcccg catatcctgt ttctagtccc
     5401 gcattgacaa tcattgccac atggcattaa tatttagcca tatgacaata gagggagtca
     5461 tgcctcaagc cgaagagcta tttaagacgt tgggaggagc cacgatattt aagcgacaag
     5521 ttcattcggc tgaagatgtt cgtgcgatta ttcataaagg ctttccctat caatcgctgg
     5581 aattcgtgct ctctaaattt catctagatt tgcccaggat gagcagaatt ctcagcgtac
     5641 caccccgtac gatggctcgg cggaaagcag agcagcgcct gacggcacag gaatctgatc
     5701 gcttagcgcg cgtagccaga atcttgacct acgcaacgaa tgtctttggt accgaagaaa
     5761 aagcgtcaac atggctcacc cggccacacc gcatcctaga aggaacagct ccaatagaac
     5821 tccttgatac cgaccttggc actcaagccg ttgaggcgat gttgggacgg ctcgaacacg
     5881 gcgtcatagg ctagtggtag tttggcgact tacgcttaag aaacatagcg ctcctgacgg
     5941 agagggcgcg agacgatatg gaggacgatg gaatacacca ggaacgccag tcgtgtacac
     6001 ctcgggcacg ctttctcttg ctgccctgga gtacctcgtc catgttgatt cggatattct
     6061 tcccgatgga ttggtcagta tcagggcgac gattcccgac ggtctcaaga tagaggctat
     6121 cagtgcctct cacttgccac tcgagtggaa agacaaaata atacccgtaa gtgtgcagca
     6181 aattggtact gattgggtta ccaatgccaa aagtgctgtc ttaaaggtac catctgtcgt
     6241 gatcgagcat gaatggaatt atgtgcttaa ccctcgccat cctgaatttt cgcagatcac
     6301 atgggacgcc gctgcagtgt tctctttcga tcagcgtctg ctgaaataaa aacctgaacc
     6361 ggcccgtcat gcggcgcgaa aaagatagtt cgcgctcttt ctcacactca acgttagtac
     6421 tgaccagcag gcatcgtccc gcgaacacac cagacgtgaa aggtaatggt cttaaaggcg
     6481 aaagtggttt ttcccgtatc gaagaacacg agccaggcgt tttttggatt gtccgtgacc
     6541 gatgtcgccg accagtagac gtccatcaac acgttggtaa aaggatgtcc ggcggggagg
     6601 gtgggtcctg atcgtgtgga ggggtccacc aaactcgcca gttctgaaat cgacggcaac
     6661 ctccagcctt tccgtccgcc tacgttctta tcggcgcaga cagcccgagc atcggtccag
     6721 gacatctcgg tcgtttctac ggtcttttcc cacacgagcc ccgtttcatc gtctcgaact
     6781 gccgcatctt cgaagagact caagaccgta aaccgcgaag cgctcggtaa cttcttgccc
     6841 caattctgtt cggaggaatg cgcggttgga ttggaatccg gggcggaacc agcatggcca
     6901 ttgcttgcca caatggtgat cccgacgagg acgagaagtc ccagcctcag catgcaattg
     6961 tgagactgcc gcatcatgct catgccgttc cctccttctg ctatgacctg tcgcagccca
     7021 ggacgacctt tcgggtgcca ataagaaaga aagcgggaac attctacgta ttcggagggg
     7081 ctgggtctat gggggaagta aggcgggcgg tcgcaccgat caactcggca cctgactcat
     7141 gacgcgaaac ggtgtatgcg gggaccgcag cgagaagctc gatagagccg tggtgaccgg
     7201 agggctgtcc aatctgaatt ggacatcgaa gtgagctgaa cccggccggt gtagcccggg
     7261 ccgggccact ctcactctaa acggtacatc acggtaagtc aggcgttttg gcaacagcct
     7321 ggttcgctgc agaggctacg atgatttcaa cacgtcgatt cttggcgcgc ccggtggcag
     7381 tagtgttggc ggcgatcggg ttgctgtccc catagccggc cgcttctaga ttggctctca
     7441 cgccgccatc ccgcagcgcc tgcgctgcac tgtttgctcg cgcatccgac agttccttat
     7501 tggaggg
//
LOCUS       DASSCQ010000064        10284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_405099, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000064 DASSCQ010000000
VERSION     DASSCQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405099"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VFH05_04500"
     CDS             complement(<1..1327)
                     /locus_tag="VFH05_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET6677503.1"
                     /translation="MRPLDRIDGYLPIEDHGIIGDGATAALIGRDGSIVWLCLPRFDS
                     PALFCSLLDRERGGHFTVALEHLQESRQYYESDSAVLVTEMRNADGVIRLTDLCPLLA
                     GADLSEDVQATRGELLRTVTALNGTVRVRIEIEPRGGAEATSRNGSLRLRCGAQPNLA
                     LHLSSSLPLNGLRSVLTLKQGQSLHLLLGWRQSHGQSAFFDPSRLYHDTVSVWRQWLT
                     RLKYQGPQEPLVRRSAITIKLLDHFENGAIVAAPTSSLPERIGGARNWDYRYTWVRDA
                     AFSVYALHRIGLSHEAAGFLSWVLDAVERDGSPRVMYNLDGKMPPSERQDSELEGYRR
                     SKPVRWGNAAASQTQHDVYGEILDCAFQWAAHHGSISDELWSQLSGLADSAAREWRTP
                     DHGIWEVRTAGRPFTYSAALCHVALDRAARMAARYGLMGPRERWQRTAED"
     gene            complement(1495..2508)
                     /locus_tag="VFH05_04505"
     CDS             complement(1495..2508)
                     /locus_tag="VFH05_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677504.1"
                     /translation="MNFTIWLIFLCSAALIVLTGSRLSRYGDEIAEFTGLGRLWIGVV
                     LMSTATSLPEVFTTVSAAWLDAPDLAAGDLFGAGMSNMLTLGLIDLMHRQKRVWREAA
                     LGHALTAALAMVLTAFAAVFVLLHVDMSHVGVGLGSLTLFLLYGLGMRLVFRQEDMER
                     RQRGQAVLIEGSSAGKSGVHRRTELRRAIFGFIVCALALLVAAPFLAWSAERIAEETG
                     VGTTFIGTSLVAITTSLPELVTAVASVRLGAFDLAVGNLFGSNAFNMAAFLFADLAYR
                     EGDLLSNLSSVHALTALWSILMMNIGLMGIIYRAEKRFILIEPDSLLMIVSYIVGLWL
                     LFR"
     gene            complement(2582..3433)
                     /locus_tag="VFH05_04510"
     CDS             complement(2582..3433)
                     /locus_tag="VFH05_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6677505.1"
                     /translation="MTRFLKALSLARGTTVNVMHVIEFPPFMNQYTSVADDWKRQASA
                     NAHKLISRVVAPLSRQGLTIRSLVEDGQPGPTLLDAVTRLGIDLTVVSPYRSSRLAKF
                     LLGSVTELLLSEMPSSVLVVRKPRREFHGELTIVLAMDFSRDAKAAAKFLLRLRLPRK
                     CRMILLHVEEAGETLLDRLSRLDTELPPAIEKAKRRRKRHTRLMLDRISGQLQQRGLL
                     VDKMLAEGIPAAQILRIADRHRADLIVMGSKGLTGLERYLLGSVSRKVARHAVCSVLV
                     VKGARRA"
     gene            complement(3472..4836)
                     /gene="nhaA"
                     /locus_tag="VFH05_04515"
     CDS             complement(3472..4836)
                     /gene="nhaA"
                     /locus_tag="VFH05_04515"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET6677506.1"
                     /translation="MNTPSRTRGSHDVRAELAPVSRLLLSAQYIIHNEVVAGGSLFVA
                     ALIALVWANSPWQGSYYAIVSIPVGFSFGSFSFSLDLRHWVNDGLMVIFFFAVGLEIK
                     REFVHGELAGWPSAVFPVGTAIGGMIIPALLYWVVNRQRETAAGWGVPIPTDIAFALG
                     VLALLGNRIPMQLRIALLALAVVDDIGAILVIALFYASSVSWMALACALAILGMIVGM
                     RWIGVRTVALYVAVGAAFWLAVQLSGVHATIAGVILGALTPASAWFSHPTFTDRIEPL
                     LRRFKSTQKQADTESAKVVLGQIETLTQETEPVLDRLQRIVSPWVSFGILPLFALVNS
                     GVTLSTEHIQEALSSPVAQGIVLGLCVGKPVGVLGASWLMVQLKVAPIPSALSWTHLW
                     GLGILAGIGFTVSLFITGLTFGSGEYAEQAKIGILTASLVAATIGSLLLYFADGRRGK
                     EGTT"
     gene            complement(5010..5309)
                     /locus_tag="VFH05_04520"
     CDS             complement(5010..5309)
                     /locus_tag="VFH05_04520"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HET6677507.1"
                     /translation="MMRGSGLGVFLLGVLAMGDLIAAMFFVKYWIRTKDRFFFYFIVS
                     FGLEVVCRYLLMYTTFNTESEPLVYSLRLLSYAIILVGIADKNRNIIRRILFQDS"
     gene            complement(6068..6159)
                     /locus_tag="VFH05_04525"
     tRNA            complement(6068..6159)
                     /locus_tag="VFH05_04525"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(6171..6410)
                     /locus_tag="VFH05_04530"
     CDS             complement(6171..6410)
                     /locus_tag="VFH05_04530"
                     /inference="COORDINATES: protein motif:HMM:NF042517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6566 family protein"
                     /protein_id="HET6677508.1"
                     /translation="MSRSVIYQRYTINSVPTQNTETGQWQLRISIFFEQDGATESIPY
                     WMPLEYATEAEADIHGITFGQRIIDGKIPGLLPRK"
     gene            7221..7646
                     /locus_tag="VFH05_04535"
     CDS             7221..7646
                     /locus_tag="VFH05_04535"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6677509.1"
                     /translation="MKLLASYHGGTRFDILSGKHRVVTDQPTADGGQDAGLSPVELFV
                     GSLASCVGYFVGKFCTRHDIPQEGLSVEAEWTMTENPHRVGHVDVSIHLPHRITPELK
                     DRLLKVAHGCTVHQSLSQAPTVGIRFTPHTHRQPADSPA"
     gene            7643..8845
                     /locus_tag="VFH05_04540"
     CDS             7643..8845
                     /locus_tag="VFH05_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HET6677510.1"
                     /translation="MKGSGELAKFSRRRWLKTLLGFGAGLAMHPAKFLAWAADMTPAR
                     DESTLTVRVSRPFDAETPVRAFTSFLTPNDHFFVRSHFGPPSAETIGESQWTLHVGGA
                     VERSNEFTMESLKQFEPVTITAVLQCSGNGRAYHRPKVPGVQWERGAVGNAQWTGVRL
                     RDVLQAAEVRAEARHVMFQGADRPVVATVPLFVRSIPLEKALHPDTLLAYEMNGRPLP
                     LLHGAPLRVITPGWMAESCIKWLTHIEVREDEASGYFMQTAYRVPVKSEQKDTGGTDN
                     ATAPVEVMPVKSLISSPEDGQAVMAGGVRIKGVAWAGETRIAKVDISIDEGNTWQPAQ
                     LVGDDRLYAWRQWQFLWQATVQGTFAIVCRATDERGHVQPAATSWNPSGFLWNGWDRV
                     TVRVHPHE"
     gene            8838..9446
                     /locus_tag="VFH05_04545"
     CDS             8838..9446
                     /locus_tag="VFH05_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6677511.1"
                     /translation="MNRSRVIVVQIGLLVFSAVMAFSQEDPESVRAMLGPRGEGLIIA
                     RCAVCHSADLITQQRLSRTRWEATIDKMKHWGAEISDQEADLLIRYLSARYHPDAPAN
                     VPPLGHEIGKGEPLKQEAQAQGPVIGVAARGAGVFEHNCQACHGGGAMGGMGPRLSKN
                     PILKLDDTFRETVLYGRGPMPAWGSVLSEQDIADVHQWLLTR"
     gene            9481..>10284
                     /locus_tag="VFH05_04550"
     CDS             9481..>10284
                     /locus_tag="VFH05_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6677512.1"
                     /translation="MPVKRRIALGCTLMVLVALMSFPRLLLGSDENRAKELLQQACVQ
                     CHRLEGQADSRFNLRAPDLIWAGSKYQRPWLIRWLTGKEAPLYAKGYRWDLSEGAMKH
                     PVVGEGDASAIADYFERHNKDPRVKVGAFDLSKVSKFEVTFGGMAYKAHACLGCHTVE
                     ENGKIIGGPQSAALQNAGQRYNMDWLYRFGQNPQDFIVHTGEFLADATDPQLRAVIGY
                     LAAQGVKDFKYYEPWTSQEFAQASVDRGQGLYKEYCAQCHGFTGKGDGPA"
BASE COUNT         2451 a         2909 c         2842 g         2082 t
ORIGIN      
        1 tatcctcagc cgtccgttgc cacctctcgc gcggccccat cagtccatag cgggcggcca
       61 tgcgcgcagc acgatccaac gccacgtggc aaagcgccgc cgaataggtg aacggccttc
      121 cggccgtacg cacttcccag ataccatggt caggcgttcg ccattcccgc gccgcggaat
      181 cggccaaccc gctcaattga ctccagagtt catccgaaat cgatccatgg tgtgctgccc
      241 attgaaacgc gcaatcaagg atttccccgt agacatcgtg ctgagtttgt gatgccgcgg
      301 cattacccca gcggaccggc ttcgaccgtc gatacccttc caactcggaa tcctgccttt
      361 cgctgggcgg cattttaccg tccagattgt acatcacgcg aggcgatcca tcgcgctcta
      421 ccgcatcgag aacccaggat agaaatcccg ccgcttcatg cgaaagaccg atccggtgca
      481 gcgcgtagac ggaaaatgcc gcatctcgaa cccatgtata gcggtaatcc caatttcttg
      541 cgcccccgat tcgctccggg agcgaactgg tcggcgccgc gacaattgcc ccgttttcga
      601 agtggtccag gagtttgatg gtgatcgcgg atcgccgaac caatggctct tgcgggccct
      661 ggtactttag gcgagtcagc cactgacgcc atacggagac agtatcgtga taaaggcggc
      721 tcggatcgaa gaacgcactt tggccgtgcg attgccgcca tcccaatagc aggtgcaggg
      781 actgcccttg cttcaaagtg agcacgcttc gcaagccgtt cagcgggaga gatgaagaga
      841 gatgcaacgc gaggttgggt tgcgccccac atcgaaggcg caggcttccg tttcgcgacg
      901 ttgcttccgc tccgcctcgc ggctcgatct cgatccgcac gcggacggtg ccattcagcg
      961 ccgtgacggt ccggagcaac tcgccccgcg tggcctgcac gtcttcggag agatccgctc
     1021 cggccagcaa ggggcagagg tcagtgagac gaatcacccc gtctgcgttg cgcatttcgg
     1081 ttaccaacac tgcgctgtcg gattcgtagt attgccgcga ctcctgtaag tgctccagtg
     1141 cgaccgtgaa gtgcccgcct cgctctctgt cgaggaggct gcagaataag gcgggggaat
     1201 cgaatcgtgg caggcagagc cacacgatgc tgccgtcgcg cccgatcaat gccgccgtcg
     1261 caccatctcc gatgatgccg tgatcttcga ttggcagata gccgtcgatg cgatcaagag
     1321 gcctcaattg ccctaccctc cccgacgtaa gcttgttgtc tcatttattc actctttcta
     1381 cccaagtgac taggccaatg actgtgcgac tggccttggc caaatgagga gatatgagta
     1441 tcatggcctc acagcagacg cgctagtttt cgtttgcatt cccgcctgcc tgcttcagcg
     1501 aaacaagagc cacagcccga cgatataact gacaatcatc agcaggctgt ccggctcgat
     1561 caaaataaat cgtttttccg cccgatagat aattcccatc aggccgatgt tcatcatgag
     1621 gatggaccaa agcgccgtga gggcgtggac tgagctgagg ttgctcaaca agtccccttc
     1681 gcgataggca agatcggcaa ataagaaggc cgccatgttg aacgcgttgc ttccgaagag
     1741 attccctacg gccaaatcga atgcgcccag tcgcacggaa gcgacggccg tcaccagctc
     1801 cggcaaggac gtcgtaatgg cgaccaatga cgtccctata aacgtggtac ccacacccgt
     1861 ctcttcggcg attcgctcgg cggaccacgc taagaaggga gcggccacga gtaatgcgag
     1921 agcacataca atgaacccga agatggcacg acgcaattcg gttcggcgat ggacgccact
     1981 cttgccggcg ctgcttcctt caattaacac cgcctgtccg cgctgccgac gctccatgtc
     2041 ttcctgccgg aacaccagcc tcatgcccag cccatagagg agaaagagcg tcagactccc
     2101 cagacccacg ccgacatggc tcatatcgac gtgtagcagc acgaatacag cggcgaacgc
     2161 agtcaacacc atggcgagcg ccgcggtgag cgcgtgtccc agcgctgcct cgcgccagac
     2221 ccgtttctgg cgatgcatga gatcgattaa acccagcgtc aacatgttgc tcatgcctgc
     2281 gccgaagaga tcgccggctg caagatccgg agcgtccagc caggcggcgc tgacggtcgt
     2341 gaaaacctcc gggagtgatg tggcggtgga catcaacacc acgccgatcc ataagcgccc
     2401 gagaccagtg aattcggcaa tctcatctcc atatcgagaa agtctgcttc ccgttagaac
     2461 aatcagagct gcggaacaca agaagataag ccagatcgta aaattcactt caggcccatc
     2521 gctgaatcgc gttttcttcg ctcatgggaa taccgccgcg ctgctgaggc acgggcgtat
     2581 gttatgctcg tctcgctccc ttgacgacaa gaaccgaaca gactgcatgc cgcgcgactt
     2641 ttcgagagac gctcccgagg agataccgct caaggccggt aagacccttt gagcccatga
     2701 caattagatc cgcccgatga cgatcggcga tgcgcagaat ttgcgccgct ggaattcctt
     2761 cggcgagcat cttatcgacg agaagtcccc gttgctggag ctgaccgctt atccgatcca
     2821 gcatgagacg ggtgtgtcgc ttccgccggc gctttgcctt ttcaatggca gggggcaatt
     2881 ctgtatccag ccgactcaaa cggtccagca gtgtctctcc cgcttcctca acatgcagaa
     2941 ggatcatgcg acatttacga gggagacgaa gccgtaatag aaacttggct gcggctttgg
     3001 catccctcga gaagtccatg gcgagcacga tggtcaattc accgtgaaac tcccttcgtg
     3061 gctttcgtac cacgagaaca gagctaggca tctcacttag cagcaattca gtgacgctac
     3121 ccaacaaaaa tttcgcgaga cgagaagaac gatagggact caccacggta agatcgatgc
     3181 ccagccgtgt tacggcgtcg agtaaggtag gacccggttg gccatcttcc accaaggagc
     3241 ggattgtgag accttgtctt gaaaggggag ccacgacgcg actgatcagt ttatgggcat
     3301 ttgccgaagc ctggcgtttc cagtcgtcag cgaccgatgt gtattgattc atgaagggag
     3361 gaaactcaat gacatgcatc acattcactg tcgtgccacg cgcgagactc agtgccttca
     3421 ggaatcgagt catgtggcta tctcgatgct gtgtcacgcc gaccaagaca ttcatgtagt
     3481 cccttccttt ccgcgtcttc catcggcgaa atacagaagc agtgacccga tggtagctgc
     3541 cacaagggaa gcggttagga taccgatctt ggcctgctcg gcgtattcac cgctgccgaa
     3601 ggtcaatccg gtaataaata aagacactgt gaaaccaatg ccggccagga ttcctaatcc
     3661 ccagagatgc gtccagctga gagcgctggg tatgggggca actttgagct gaaccatgag
     3721 ccaggaggct ccgagcacac caaccggctt gccgacacac aagcccaaca cgatgccctg
     3781 cgcgacggga ctcgagagcg cttcttgaat atgctcagtt gacaaggtca cgccagaatt
     3841 gactaaggcg aacaacggca gtattccaaa gctcacccat ggggagacga tgcgctggag
     3901 ccggtcgagc accggctcgg tttcttgggt gagggtttca atttgaccga gcaccacctt
     3961 ggccgattcg gtatccgctt gcttctgagt gctcttgaat cgccgtaata acggttcaat
     4021 gcgatcggta aaggtgggat ggctgaacca cgctgaagca ggagtgagag cacccagtat
     4081 gacgccggca atagtggcat ggacgccaga taattggaca gccagccaaa atgccgctcc
     4141 cacagctaca tacaacgcga cggttcgcac gccgatccag cgcatgccaa cgatcattcc
     4201 caaaatggca agcgcgcaag caagtgccat ccaggatacg cttgatgcat aaaacagtgc
     4261 gatgaccaga atggcaccga tgtcatccac caccgcaagg gcgagaaggg caatccgcag
     4321 ttgcatgggg atccggttgc ctagtaacgc caacacgcca agcgcaaacg caatgtcagt
     4381 agggataggc actccccatc ctgcggcggt ctcgcgttgg cgattcacga cccaatacaa
     4441 caaggcagga atgatcatgc cacctatggc agtacctaca gggaagaccg cactcggcca
     4501 cccagcgagt tcgccatgga caaattcccg tttgatttcc aatcccacgg caaagaaaaa
     4561 aatcaccatg aggccatcat tcacccaatg ccggagatcg agtgagaagc tgaaggaacc
     4621 gaaagaaaac ccaaccggta tcgaaactat cgcgtaatag cttccctgcc atggcgaatt
     4681 tgcccagacg agcgcaatga gagcggccac gaacaacgac cctccagcca ccacttcgtt
     4741 gtgaatgatg tactgtgcag ataagagcag tcggctgacg ggagcgagtt ctgcgcgcac
     4801 atcatgagac ccgcgtgttc gagatggagt attcatccgt gtccttctct attccgctaa
     4861 acggcaacac ctgaacggat actctgaagg caagtcgtga gatcgacttt gacagaacgc
     4921 gcgatagtcg ctaaggacct tccgtgcaca acaacctgtt cctagacttt cacgcgagaa
     4981 cagagagtaa acagacctgc tcgcaggagc tagctgtcct gaaacaatat tctccgaata
     5041 atgttgcggt tcttgtccgc tatgccaacc agtatgatcg cgtaagagag gagtcgcagg
     5101 gaatagacca gtggttctga ttccgtattg aaagtcgtat acatcagcag gtaccggcaa
     5161 acgacttcta acccgaagga tacgatgaag tagaaaaaga acctgtcctt ggttcttatc
     5221 caatacttca caaagaacat cgcggcgatc aaatcgccca tcgccagcac accgagcaaa
     5281 aacaccccta agccgcttcc cctcatcatc gaggctcctc ctccgaataa atcaagccgt
     5341 gaaggagaaa gcataaggct gtcaaggcga ctagatacct cgggatactg agatcaagct
     5401 ctaaggggaa gatgatcttg tccatcacaa gaagaagatt gttcagggta gagataagga
     5461 agaacaggcc gcaccagaat aaaaaccgat agtctgtctt gcgatagccg ttcagaagaa
     5521 acatggcgca aagggccgag gtcacggcac agagaccata gactacagat gccacaatta
     5581 gtcccctttc ttattaatgt cgaaggcccg cgagaattcc tgaatccgac ttctgggctt
     5641 ggagtgcacg agattggaga tgggcacgac gtgtctagcg taatagtagg caaggcgatc
     5701 gaccatctct cgtaggtccc cccgtgaagg acagtaccga taccgatgtc ccttctcagt
     5761 gtgctctctc agtaacagtc cggcggtatg aagtcccgtc gcaatgggtt gacagtcctt
     5821 aacggagata tacagtcgct cagcaatcct ctcctcttcc caccagctat cagggcttcc
     5881 acgcagtagc aggaggcact ccaaatgccc aatgctatcg attctttcta agatgaatcg
     5941 ctggacgtct gaagggatta tgggttccat gttctcaaca tcccggtgca cgccgaaaac
     6001 cactcacttg ggcctttgtt cagcaagcca acgcccatac aaaaagcgct cccggcaccg
     6061 caagcgctgg cggagagggt aggattcgaa cctacggtcc gggattccgg acagcagcat
     6121 aagaacgtct gctcgattca gccactctcg cacctctccg actgttcttt tcatttcctc
     6181 ggtaaaagcc cgggaatttt gccgtcaata atccgctggc caaaggtgat gccatgaata
     6241 tcggcttccg cttccgttgc atactccagt ggcatccaat acggtatgga ttcggtagca
     6301 ccgtcctgtt caaagaaaat cgaaattcga agttgccact ggcctgtctc ggtattttgc
     6361 gtaggcacag agttaatggt gtaacgctgg tagatgacgc tgcgactcat cagaacactt
     6421 cattggcttg tgcaccgttg aagaacggaa agcgcatcga gccgaaatga aagaattcaa
     6481 aaccccaacc atgcaggtga ggctaagaga gaggaggatg gaaaagcgtg tcctggcgaa
     6541 gctgttggta actggccctt atcatggcaa caccgagcag acattggaga agaactaaga
     6601 aaaccgactc atttccgaga acagcaaagc tcgttaagat ggaactcatg aaaggatcgt
     6661 ccgagccatc aacatcccag aacgccactg ctgctccaag gatttggagg aaaacgccga
     6721 cacagagcag agcaataatc aaaacacgga ggactcgagt taacatcgca gtgtaatgaa
     6781 cttccaccat cgccaactcc tcataacctg cgtgcgtcgc tacagccagc agattagcac
     6841 agagcatgaa gtttatatac accaacgtcg caagccatcc ctaatttttc ctgcaaacgc
     6901 ctggcagtct atattcttca ttagttccgt tcctagttca taccgagggt atccactcac
     6961 atgaatgccc ctcagaagaa gcgatgatca taatcatggc tttacgacgt catcgttccg
     7021 gaacttcttc ttggcttgcg gtaatattgg tgtggcaaat tgcaccattg atttgattcc
     7081 tcgtggcgcg tttatcgtca agcgatcaga tgcctcgggt tctatccgcc taagcgcttg
     7141 ttaatcggtg gggtcgtctc ctaggcacag acgtatgaga cggcacgcgg ttcgcaaacg
     7201 tcgagtgtag agaggacatc atgaaacttt tagcctcgta tcatggcggc acgcgtttcg
     7261 acattctcag tggaaagcat cgggtcgtga ccgatcagcc gactgctgat ggcggccaag
     7321 atgccggcct tagcccggtc gaactttttg tcgggtctct tgcgagctgt gtcggctatt
     7381 ttgtcgggaa attctgcacg agacacgaca ttccgcaaga ggggttgagt gtagaagcgg
     7441 aatggaccat gacagagaac ccgcatcgag tgggccatgt agacgtttcc attcatcttc
     7501 ctcatcgcat cacgcctgaa ttgaaagacc ggcttctgaa agtggcccac ggctgcacgg
     7561 tgcatcagtc gctttcgcag gcgccaactg tcgggatcag attcacgcca catacccatc
     7621 gtcaaccagc ggacagtccg gcatgaaggg atccggcgaa ctcgcaaaat tctctcgtcg
     7681 acggtggttg aagaccttac tggggttcgg tgccggcctc gccatgcatc ccgctaagtt
     7741 cctggcctgg gccgctgaca tgacccccgc aagagacgaa tctacactca ccgtgcgtgt
     7801 ctcgagacct ttcgacgcag aaactccggt ccgagcattc acgtcattcc tgacccctaa
     7861 tgaccatttt ttcgtacgaa gccattttgg ccctccctcg gctgagacga tcggagaatc
     7921 gcagtggaca ctccatgtgg ggggcgccgt cgagcgctcg aacgagttca ccatggaatc
     7981 tttgaagcag ttcgagccgg tgacaattac ggctgtgctt caatgcagcg gaaatggccg
     8041 ggcctatcat cggccaaaag tacctggggt ccaatgggag cgaggcgcag tcggcaacgc
     8101 gcaatggacc ggggtgcgtc tccgtgacgt cttgcaggcg gctgaggtgc gtgcagaagc
     8161 ccggcatgtg atgtttcaag gcgcagaccg accggtcgtc gccacggtcc cgctgtttgt
     8221 gcgaagcatt ccgctcgaga aggcccttca tcccgacacc ctgctcgcct acgagatgaa
     8281 tggtcgccca ttgccgctcc ttcatggcgc tccgctgaga gtgatcacac cgggctggat
     8341 ggcggagtcc tgcatcaaat ggctgactca catcgaggtg cgcgaagatg aggccagtgg
     8401 ctacttcatg cagacggcct atcgggtgcc tgtgaagtcg gagcaaaagg atacaggtgg
     8461 gaccgacaac gccacggctc ctgtggaagt gatgccggtc aagtcgctca tctcgtctcc
     8521 tgaggatggc caggcggtca tggccggcgg cgtgagaatc aaaggcgtag cctgggccgg
     8581 agaaacaagg attgccaaag ttgacatatc catcgacgaa gggaacacat ggcagccggc
     8641 acaactggtc ggcgacgatc ggctttatgc gtggcggcag tggcagtttc tctggcaggc
     8701 cacggtccag ggcacgtttg caatagtctg ccgcgcgacc gatgagcgtg gccatgttca
     8761 accggccgcg acgtcatgga atccaagcgg tttcttgtgg aacggatggg atcgcgtcac
     8821 ggttcgagtt catccacatg aatagatcgc gcgtcatcgt tgtgcagatc ggcctcctgg
     8881 tcttctcggc cgtcatggcc ttttcgcaag aggatcccga gtcggtacgt gcaatgctgg
     8941 gtccgcgtgg agaggggctc atcatcgccc gctgtgctgt ttgccacagt gcggatctca
     9001 tcacgcagca gcgattgtcc cgtacgcggt gggaagcgac gatcgacaaa atgaagcatt
     9061 ggggggccga gatctccgac caagaggccg acctgctgat acggtatcta tcggctcgct
     9121 atcatcctga tgcgcccgcc aatgtaccgc cgctcggtca cgagatcgga aaaggcgaac
     9181 ccttgaagca ggaggcgcag gcgcaaggcc ccgtcatagg cgtcgcggcg agaggagcag
     9241 gagttttcga gcacaattgc caggcttgcc atggaggcgg cgcgatgggt ggcatgggac
     9301 cgaggctctc caagaatcct attctgaaac tagacgatac gttccgtgag acggtccttt
     9361 acggaagagg cccgatgccg gcttggggtt cggtgctcag cgaacaggat atcgcagatg
     9421 tccatcagtg gctgctgaca aggtagaggt cagaggctgc tccaggaaag gagtgtcgaa
     9481 atgccggtga aaaggagaat tgcccttgga tgcacgctca tggtgctggt cgccctcatg
     9541 tcgtttccca gactgttgct cgggagcgat gagaatcgcg caaaggaatt gcttcaacaa
     9601 gcctgcgtcc aatgtcatcg cctcgagggt caggcagatt cacgcttcaa tctgagagca
     9661 cctgatctta tttgggctgg cagcaaatac cagcgcccat ggctcatccg ctggctgacc
     9721 ggcaaggaag ccccattgta cgccaaaggc tatcggtggg atctcagcga aggagccatg
     9781 aaacaccctg tggtcggcga aggcgacgcg agcgccattg ccgactattt cgagaggcac
     9841 aacaaggacc cccgcgtgaa agtcggggct ttcgacctgt cgaaggtgag taagttcgag
     9901 gtgaccttcg gcgggatggc ttacaaggcg cacgcctgcc ttggctgcca tacagtcgag
     9961 gagaacggca agatcatcgg cggtccgcag agtgcggcgc ttcaaaatgc cggccagcgg
    10021 tacaacatgg actggttgta tcgtttcggg cagaacccgc aggacttcat cgtccatacg
    10081 ggagaatttc tcgcggacgc gactgaccca caattgcgag cggtgatcgg ttaccttgcc
    10141 gcgcagggtg tcaaagactt caaatactat gagccctgga cgagtcagga attcgcgcag
    10201 gcaagcgtcg atcgcggcca ggggttgtac aaagagtatt gcgcacaatg ccatggcttt
    10261 acgggaaagg gcgacggacc tgcc
//
LOCUS       DASSCQ010000065         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_80707, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000065 DASSCQ010000000
VERSION     DASSCQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80707"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFH05_04555"
     CDS             <1..213
                     /locus_tag="VFH05_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677513.1"
                     /translation="PLSRKEVAMTQELVLVVLLTGLLGLIWIMTLGTVDDDTAGAQSH
                     EPETASSTQTDDARPESALNRRTIAA"
     gene            210..578
                     /locus_tag="VFH05_04560"
     CDS             210..578
                     /locus_tag="VFH05_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677514.1"
                     /translation="MNQHAVRPPHREATMLSSVETSVGKPVSVHQDPSPTRSRKQRVT
                     ITLPLSLLERLRNAVYWTGHGPLARLIADALDDAVTHMETSNGQAFPQRLSPLKRGRP
                     RTKTPSVVLPLPPKFSGRIQ"
     gene            775..>2391
                     /locus_tag="VFH05_04565"
     CDS             775..>2391
                     /locus_tag="VFH05_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6677515.1"
                     /translation="MASELARIGHPMLATLLTVGLASMAQAEPALPEAVEPRPEVALT
                     EELELLKEEETVSIASRYEQPISQAASNVYVITDEDIRQSGATDLPTVLRRIPGMEVM
                     QTTGADFNVSMRGDNQLNANKLLVMVDGRSIYVDAHAQMYWKAIPVTLPEIKRIEVLR
                     GPASALYGFNAFDGVINIITKSPEEMRGTTVQFGGGGLGTITGAAIHAGTVNKFGYRL
                     SVGHDQNQQWRDRDSLAFRATKFNVQTDYRLPGESKLSFSGGLVDANRYDGPITTTGV
                     TLSSSTPALGYAHVLYERPNFFIRGFWNRWDDSVGLNTYPLLSDFLRFTDRNLNPFLN
                     FRGDTYNVEAQHSVELFTDHRLTYGLNYRLNTLSSNLLDDNYNQENRLGIYVQDEWKA
                     TDRFSVVAGVRYDLDTFIHPTLSPRVTLLYRPATDHTFTLGVSKAYRPPTLVESYFNS
                     LGFVTLPPPLPSPPPTVGRGSTNLGPEEIISYEAGYQGWYWNHRLRLRANVFFNHITD
                     LIIPTTPLPTQGGSADIYGGEAGVEFVLTRW"
BASE COUNT          512 a          709 c          634 g          536 t
ORIGIN      
        1 ccgctttctc ggaaggaggt cgcaatgaca caagaactgg tgcttgtcgt cctacttacc
       61 ggcttgctcg gccttatctg gatcatgacg ctcggcaccg tggacgatga caccgccggc
      121 gcgcagagcc atgaaccaga gaccgcttct tcaacccaga ctgacgacgc tcggccagag
      181 agcgcgttga accgccgtac gattgcagca tgaatcaaca cgccgtccgt cctccacatc
      241 gtgaggccac catgctttct tcggttgaga catcagtcgg taagcctgtg agcgtgcatc
      301 aagacccctc cccgacaaga tctcgcaagc agcgagtgac cattacgttg cccctctcgc
      361 tgctcgaacg ccttcgtaat gccgtctact ggaccggcca tggccccttg gcacgtctga
      421 ttgccgatgc cttggacgac gccgtgacgc acatggagac atcgaacggc caagcctttc
      481 cccaacgcct ctcaccgctg aaacgcggcc gccctcgcac gaagacgcca tcagtggtgc
      541 ttcccttacc tccgaagttt tcaggacgca ttcaataagc ttccagcaat tccttcggct
      601 gggccttcgc acggcttggc ttgccaggaa actaatccgc ctcattccgt tcattatcgg
      661 cccgaccact ctgagcatgt gttttgcttc agtctcacat ccctgaaatc ttcgtacata
      721 gaagtatggt gatcacccgt cggcactaca ctcgtagccc gtttgaaagc gctcatggct
      781 agcgagctgg cacgaatcgg ccatccgatg ctggcgacct tgctcactgt ggggttggcg
      841 tcgatggcac aggctgagcc ggcgctgccg gaggcagtgg aaccccggcc ggaagtcgcg
      901 ctgacagagg agctggagct cctgaaagaa gaagagacgg tgagtattgc gtcgcggtac
      961 gaacagccga tctcccaggc ggcatccaac gtctacgtca tcaccgatga agacattcga
     1021 caatccggag ccacggattt gccgaccgtt ctgcgccgta ttcccggcat ggaagtgatg
     1081 cagacgacgg gcgcagattt taatgtcagc atgcgaggcg acaaccaatt gaacgccaac
     1141 aagttgcttg tcatggtgga cgggcgatcc atctatgtcg atgcgcatgc gcagatgtat
     1201 tggaaggcga tcccggtcac tctgcctgaa attaagcgaa ttgaggtcct ccgaggccca
     1261 gcgtccgctc tttatggatt taatgcattc gatggggtga tcaatatcat caccaagtcg
     1321 cccgaggaga tgaggggcac gacggtacaa ttcggcggcg gcgggttagg gacgataacc
     1381 ggagcggcaa ttcatgccgg cacggtcaat aagttcgggt accgcctctc cgtgggccat
     1441 gatcaaaacc agcaatggcg cgaccgggat tccctggcgt ttcgcgcaac caaattcaac
     1501 gtgcagacgg actatcggct gccgggcgaa tcgaagctct ctttttccgg cggactggtc
     1561 gacgcgaatc gttatgatgg accaattacc accacaggtg tgacgctatc ttcctcaacg
     1621 ccggcgcttg gctatgcaca cgtgttgtat gagcggccga acttcttcat ccgcggattt
     1681 tggaatcgat gggatgactc cgtcggcctg aacacgtatc cgttgctcag cgatttcctt
     1741 cgcttcaccg atcggaacct caatccattt ctgaacttcc gcggcgatac gtataatgtc
     1801 gaagcgcagc attcagtcga gttgttcact gatcaccggc tgacgtacgg actcaattat
     1861 cgtctcaata cgctctcgag taatttactc gacgataact acaatcagga aaaccgtctc
     1921 gggatctatg tccaggatga atggaaagcg actgaccgat tcagtgtggt ggcaggcgtg
     1981 cggtacgacc tggatacctt cattcatcct acgctgagtc ctcgcgttac cctgctgtac
     2041 cggcctgcga cagaccatac ctttactctt ggggtttcaa aagcctaccg cccacccacg
     2101 ctggtggagt cttactttaa ttcgctcggc tttgtcacac tccctcctcc gctgccatcg
     2161 cctccaccga ctgtgggaag aggttctacc aacctcggtc ctgaagagat catttcctat
     2221 gaggccggct atcaaggctg gtattggaac catcgcctgc gacttcgggc taacgtgttc
     2281 ttcaatcaca ttacggatct gattattccc acgactccct tacccacaca aggtggatcg
     2341 gccgacatct atggtggaga agccggtgtg gaatttgtgc tcacgcgttg g
//
LOCUS       DASSCQ010000066         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1761416, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000066 DASSCQ010000000
VERSION     DASSCQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761416"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFH05_04570"
     CDS             complement(<1..756)
                     /locus_tag="VFH05_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET6677516.1"
                     /translation="MFLSRRQFLKVSAGTVAAVAVADKVLALTALQPVIEVGNPLGEY
                     PDRSWERVYHDQYRYDSSFTWVCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDLY
                     GNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKEWMDDGAPELTPDVKQKYK
                     FNRRFLDDMMRVSWDTAFTYAAKGMIVIATRYSGEAGARRLREQGYAPEMIEMMKGAG
                     VRCFKHRAGMPVLGIVGKMGNTRMNGGVNALLDS"
     gene            complement(1242..2711)
                     /locus_tag="VFH05_04575"
     CDS             complement(1242..2711)
                     /locus_tag="VFH05_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6677517.1"
                     /translation="MIESDALNLRTSEKRSKTPFTILVVEDNPTDIELMLHTLEQADI
                     KPLGGDFEMEVRANAEGALQLLGERSVDLVLTDLMLPGMSGLDLVSRIQALDPNLPVL
                     VVTRVNAVAQAVDAIRRGAFDYVLKPVNPEDLGVRLHRAIRISEILRRHAIYEHHDRQ
                     EYEAHSLVGVSPAFEGIVRGIRTAAQTRSTVLITGETGTGKGLIARAIHQQSHEQQQP
                     FQVIDCTTVPEGMMESELFGHVRGAFTGAVSDKRGLIELANGGTVFFDEIGELPLPLQ
                     AKLLRVLEENEVRPLGGTRVRRVDMRFIAATNRNLEARVREGLFRKDLYYRLAVVQII
                     IPPLRERPEDIRALARHLLGKLSRELGRARCHFDEDALNVLAAYDWPGNGRELRNVIE
                     RAILLATADTIAAQDLHALLPKLAGDFTNSADGALTSLPYMEAKERAIEQFTKAYLQA
                     KLSQHAGVITKAAESSGIPRQHFSLLMKRFLGSDLPGEE"
     gene            complement(2708..>4470)
                     /locus_tag="VFH05_04580"
     CDS             complement(2708..>4470)
                     /locus_tag="VFH05_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677518.1"
                     /translation="ADMLIPIEQQLKNFLRSKHELITEVQQGNADNALAYVQSGEGLR
                     MSDRFRQELRSIEDQLAHRRELLNEQGIALSKATFIGLWLALAGVMVLGWTSSRMLAR
                     SLTQPLTRLHSATRMIEAEVDTAGITKLLSSGDVSKDELGQLEEAFLGMARRIAEHVH
                     ELEALNTIGHEINTIGPDGLEGVLRRITDRAVELVEADACMVFLRDDRMGCWVVEAAS
                     GEWNERLKKSVMLWEELPVCVEAFETRAAATGNHFQSDERPEVLRRNLLGDSMLAIPL
                     LSQGTPFGVLSLSSRTARPAHEWNQRLAKGLTQEAALAISNARLYEAVQQKQRRLLAR
                     LRQLEHLAEALAHDLKGPGARMEELAKLLAQKFAGHVDERTAKWLRLLQENGKDIVER
                     VEGILEVARVGTGQGAVTAVDPNVVLEGVLKTYAGEIERQGALVHVEARFPLVACHGA
                     YLRQIMDNLVSNALKYPRPGEPPRVRITSTTGKHMVCIAVQDHGIGIPMEHRLRVFQP
                     FVRLRPSDTPGSGIGLSIVQRIVALYGGHVWIDGEEGEGCTVKFTLPWLRNDLTSDQA
                     QPSQIEVLDLPKNGSLRGET"
BASE COUNT          936 a         1330 c         1221 g          983 t
ORIGIN      
        1 ggagtcgagc agggcgttga cgccaccgtt catcctggtg ttgcccattt tccctacgat
       61 gccgagcacc ggcataccgg ctcgatgctt gaagcagcgc acccccgcgc ccttcatcat
      121 ctcgatcatt tccggcgcat agccctgttc ccgcaaccgt cgcgctcccg cttccccgct
      181 gtagcgggtg gcaatgacga tcatgccttt cgcggcatag gtgaacgccg tatcccaaga
      241 gacccgcatc atgtcgtcca agaatctgcg gttgaatttg tatttttgct tcacgtccgg
      301 ggtcaattct ggcgcgccat cgtccatcca ttccttccag cctttccgca tcaacggtcc
      361 cttcaagcga taggggccgt agactcgacg atggaaggtg aaccctttca agcacatgcg
      421 cgggttatgg gcgaatgttc cgcggttgcc gtagaggtct tcgtaggtct ggtggtcgta
      481 gttctgctca acccgcatga caaccccatt gcggacgaac gcccgaatcc ggcaggcgtg
      541 cgtgtcgttc ggggagcaaa cccatgtgaa tgacgaatca tagcggtact gatcgtggta
      601 gacgcgctcc caggaccggt ccggatattc accgaggggg ttgcccacct cgatgaccgg
      661 ctgcagcgcc gtcaaggcca gcaccttatc ggccaccgcg acggcggcaa ccgtcccagc
      721 ggagactttt aaaaactgcc gacgtgacaa aaacattgtg tttacctccc tgctaaaaga
      781 tcgatctcac atgctgctgc ccagtcgaac atctcacctc cttttgctct caagttagtt
      841 tcttcacctt tggtgactct ccgaaacccc agctacagca aggagattgc cagcaattgc
      901 agaggacggc ccctgcctaa gcggattgtt taagtgatcg attttaaaag tgaaaatggc
      961 aaacgcgagg cgaatggtat tcgtgtggag gctcggagga acccctaaaa ggcataggga
     1021 gatatgcgtt gacctaatat ccgccgcaac atcttgatat tacgcgacat cattgcgcat
     1081 atcgccatgc acgaatcagg atttttattg tgattgcgtg tgattgggca gctggagggc
     1141 gacgcggcga gggattcgtt actacggggc tacgagattg cgaggtgaag gtcgcgaaag
     1201 gagaagacgg ttggcgatcg cgttaccaga aggacgcggg cttactcttc gcctgggaga
     1261 tcgcttccca gaaaccgctt catcaataaa gaaaagtgct gccgcggaat accgctgctt
     1321 tctgctgcct tggtgatcac acccgcatgt tgagacaatt tcgcctgaag gtaggcttta
     1381 gtgaactgct caatggcacg ttccttggct tccatataag gtagcgaggt cagcgctccg
     1441 tcagcagagt tcgtgaaatc gccggctaac ttcgggagca atgcgtgaag atcttgggcg
     1501 gcaatcgtat cggctgtcgc caacaatatg gcacgctcga tgacgttgcg cagctcccga
     1561 ccgttaccag gccaatcgta agcggccagg acattcaacg catcttcatc gaaatggcaa
     1621 cgcgctcttc ccaactcgcg gctcaatttc cccagaaggt ggcgagccag agcgcggata
     1681 tcctccgggc gttctctcag cggagggatg atgatctgga cgactgcgag acggtaatac
     1741 agatccttgc ggaacaatcc ttctcggaca cgtgcctcca ggtttcgatt agtcgccgcg
     1801 ataaatcgca tgtcaactcg acggacccgc gtgccgccca agggccgcac ttcgttctct
     1861 tccagcacgc gcaataactt cgcctgcagg gggagcggaa gctctccgat ctcgtcaaaa
     1921 aatacggtgc cgccgttggc gagctcgatc aagccccgct tgtcggacac agcgccagtg
     1981 aaagcgcctc ggacatgacc aaacagctcg ctctccatca tgccttccgg taccgttgtg
     2041 cagtcgatca cctggaacgg ctgttgctgt tcatgacttt gttgatggat tgcccgggcg
     2101 atcagaccct ttcccgtccc cgtttctccc gtaatcaaga cagtggaacg ggtctgcgcg
     2161 gcggttcgga ttccccgaac aatgccttcg aaggccggac tcacaccgac caggctatgg
     2221 gcttcatact cctgccggtc atggtgctca tagatggcgt ggcgacgcag gatctctgaa
     2281 atgcgtatgg ctcgatgcag tcgcaccccc aaatcttccg ggttaaccgg cttcaggaca
     2341 tagtcaaagg cgccgcgacg gatcgcatcg acggcctgtg ccaccgcatt gactctcgtg
     2401 acgacgagga ccggcagatt ggggtcgaga gcttggatgc ggctgaccaa atcaaggcca
     2461 ctcatcccag gcagcatgag gtccgtcagg acgagatcaa cagaccgttc gccaagcaac
     2521 tggagggcgc cttcggcatt ggcgcgaacc tccatttcaa agtcgccccc gagcggtttt
     2581 atgtcggctt gttccagcgt gtggagcatc agctcgatat ctgtcgggtt gtcttcgacg
     2641 accaggatgg tgaagggcgt cttcgatcgc ttctcagaag tccgcagatt cagtgcatcg
     2701 ctctcgatca tgtttcacct cggagggagc cgtttttagg cagatccagc acttcgattt
     2761 gagagggctg agcctggtcc gacgtcaggt cgtttcgaag ccatggcagg gtgaacttga
     2821 ccgtacaacc ctctccttcc tccccgtcaa tccatacgtg gccaccgtag agggccacga
     2881 tgcgctgtac aattgagaga ccaatgccgc tccccggagt atctgacggg cgcagtctga
     2941 caaagggctg gaagacgcgc agccgatgct ccatgggaat gccaatgccg tgatcctgca
     3001 cggcaataca aaccatgtgt ttgccggtcg tcgatgtaat cctcactctc ggcggctcac
     3061 cggggcgcgg atacttcaac gcatttgaaa ccaagttatc catgatctgt cggaggtagg
     3121 ccccgtggca ggccaccaat ggaaaacggg cctcgacatg aaccaatgcc ccctgtcgtt
     3181 cgatttcgcc ggcgtaggtc ttcagcacgc cttcgaggac cacatttgga tccaccgccg
     3241 tgactgctcc ttgtccagtg cccacgcgcg ccacttcgag aataccttcg actcgctcca
     3301 caatgtcctt gccgttctct tgaagcagtc tcagccattt tgccgtacgc tcatcgacgt
     3361 gacctgcgaa tttctgagcc aacaacttgg ctaactcttc catgcgtgca ccgggcccct
     3421 tcagatcgtg cgccagtgct tcggccaggt gctcaagctg ccgtaaccga gcgagcaatc
     3481 gtctttgctt ctgctgcacc gcttcataca gtctggcatt ggaaatcgcg agcgccgctt
     3541 cctgtgtgag ccctttggcc aggcgctggt tccattcatg cgcaggcctt gccgtccgac
     3601 tcgagagcga caggacaccg aatggagttc cctgagacag cagcggaatg gcaagcatgc
     3661 tgtctccaag cagattacgc cgtaagacct ccggccgctc gtccgattga aagtgatttc
     3721 ctgtagcggc ggcccgcgtc tcgaaggctt cgacacagac cggcaactcc tcccagagca
     3781 taactgattt cttcaagcgc tcgttccatt ctcccgaggc cgcctccacg acccaacaac
     3841 ccatccgatc atcccggagg aagaccatac atgcgtcggc ttcgaccagc tcgacggctc
     3901 ggtccgtaat gcgccgaagc actccctcga gaccatctgg gccgattgtg ttgatttcgt
     3961 gtccgatggt attgagcgcc tcgagttcgt ggacatgctc ggcgatccgc cgcgccatgc
     4021 ctagaaaggc ttcttcgagc tggcccagtt catctttcga gacgtctccg gaagacaaga
     4081 gcttggtaat tccagcagta tcgacctccg cttcgatcat acgggtggct gaatgcaggc
     4141 gcgtcaaagg ctgcgtcaac gaacgggcca gcatacgaga gcttgtccag ccaagaacca
     4201 tgactccggc aagggccagc caaaggccga tgaatgttgc tttggagagt gcgatgccct
     4261 gctcattgag caattcccgt cggtgtgcca attgatcttc tattgaacgc aattcctgac
     4321 ggaaacgatc cgacatcctg aggccctcgc ctgactgcac ataggcaagg gcattgtcag
     4381 cattgccctg ctgcacttcg gtgatgagct catgcttcga acgcaagaag ttttttagct
     4441 gctgctcgat cggtatcagc atgtcggcag
//
LOCUS       DASSCQ010000067         6469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1207877, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000067 DASSCQ010000000
VERSION     DASSCQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207877"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFH05_04585"
     CDS             complement(<1..227)
                     /locus_tag="VFH05_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677519.1"
                     /translation="MSPRSNRRGHRRRQSRRRVTRKKLWRFIRLRWRDMRAFVGAVWA
                     AHPAVAIPVIALLLGVIWFGINWTYHATKKP"
     gene            complement(450..842)
                     /locus_tag="VFH05_04590"
     CDS             complement(450..842)
                     /locus_tag="VFH05_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677520.1"
                     /translation="MKQVFLALSVMLTCLVTVVACDDGKPAGTATTAVGADIALMAPA
                     SSPGRAINDEGVGHYKQGHWDVASEGFKKAIKADANLAEAHYNLALSLDKMGKHEDAT
                     GSFKKAAELAPANPAIKDSPILKKHLGM"
     gene            complement(936..1961)
                     /locus_tag="VFH05_04595"
     CDS             complement(936..1961)
                     /locus_tag="VFH05_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6677521.1"
                     /translation="MKPLKVLVLMHKHLVPPQNTSDVDTEKAEWKTEFDVVTTLRAIG
                     HEAEPLGVEQDLGIIRSTIDTWKPHIVFNLLEAFHNVSLFEPNVVSYLELLRIPYTGC
                     NPRGLLIAKDKGLSKKLMAYHRIPVPEFAIFHRGLKIRLPKRLAFPLIVKSLTEESSL
                     GISQASVVEDEQKLRERVGFIHESVGTDALVEQFIEGRELYVGVMGNQRLRVFPVWEM
                     QFSKMPDNVHRIATERVKWSNKYQEKYGIQTAELKDASDSVVERIQHLAKRVYRSLDL
                     SGYARIDLRMDTDEKLYVLEANPNPQIAEGEDFAESAKQAGVPYKQLLQQIITLGLQW
                     RPERNGG"
     gene            complement(1958..3031)
                     /locus_tag="VFH05_04600"
     CDS             complement(1958..3031)
                     /locus_tag="VFH05_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET6677522.1"
                     /translation="MAIQDESEPAPDVSPDTRRNDLTDWATWPDERLLDLRLCDLDVK
                     IAGSNLEDRIAQLYQELDDRGLRFHPHFWLSDEWFCPDDVPGIAIPFYLAHPRLAKLE
                     LAQMLEVEGGDPDSCMRILRHEAGHAIENAFKLRRRRHRQQLFGNSSEPYPDDYSPKP
                     YSKSFVLHLDSWYAQSHPDEDFAETFAVWLTPNSLWETRYAGWPALKKLQYVDQLMQT
                     LVGKPPLNVSTEEIDPLRRLRTTLRRHYKKKRRHYGVDHPDFNARDLQRFFSEATEHA
                     GNMTAIRFLMKIRKPVRRLVAGWTGVYQYTIDQVFEDLIDYCRPLNLRLAVPEEQAKQ
                     EFTVFLTVQAMNYVHTGGRRVAL"
     gene            3350..4006
                     /locus_tag="VFH05_04605"
     CDS             3350..4006
                     /locus_tag="VFH05_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677523.1"
                     /translation="MLRWFALRTSVIVLMAGCMSPGASNPRHDVTAVSYRTDATKGLV
                     VLSALWGRSWKCGTFEHGQLRAFGFDRLPSVTEAEDATVDVVIEDSTDLRGGPAANYV
                     LALEPGEYGLAMFAIRVASSTTDMRTVSVGRARLFEKGEPVGGTFTVKAGELVYIGHF
                     GLDCSKEPTIWRYYLDGRGGFEHYKRLIKDQFPFLPVEAMQMRLFKTSTLGRDYVQPQ
                     "
     gene            4022..5662
                     /locus_tag="VFH05_04610"
     CDS             4022..5662
                     /locus_tag="VFH05_04610"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET6677524.1"
                     /translation="MKAAELLVRCLENEGVDLVFGLPGEETLELTDALLGSRTRFIEA
                     RHEQGAAFMADVYGRLSGKAGVCLSTLGPGATNLLTGVADAFLDRAPLVAITGQASLN
                     RRHKESHQYIDVMSMFKPVTKWSASIPKAEVIPEAVRKAFKIAQTEKPGATHLELPED
                     VAEEQIGDASQLQPLFVQAPVMPEPSPAQVARAVRTISGAQRPVILAGNGVIRSRAHE
                     AVRQFARRVHIPVLHTFMAKGTLPDSEPLSLYTIGLLARDYTSTVMEQADVVIAIGYD
                     FVEYAPCFWNPHRDKRIVHIDGSPAEVDEHYIVEVGLLGDLRHSLDLIGAAVPPFDLS
                     WSSISRKTVLDGFEAEVAGSPSWPLRPQHLMRELRAALRPDDLVICDVGAHKLWMARM
                     FPCEVPNSCIISNGFAAMGIAVPGAIAAKLSFPERRVVAVTGDGGFLMNSQELETAVR
                     LALPLVVLVWRDNGYGVIRWKQQLRFGRTSAVEFGNPDLVQYARSFGAIGYRVAEPSE
                     LRPVLEEALKSKVPAVIDCPVDYAENLRLTERLQALPH"
     gene            complement(5796..6023)
                     /locus_tag="VFH05_04615"
     CDS             complement(5796..6023)
                     /locus_tag="VFH05_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677525.1"
                     /translation="MKVLFHLLIVLLLSWPDWALAEEAPHAGYRGIASIYYTLIWGIL
                     AYGLWDAFGKKAFYVGGPVIAVIIYFLLPSS"
     gene            complement(6190..>6469)
                     /locus_tag="VFH05_04620"
     CDS             complement(6190..>6469)
                     /locus_tag="VFH05_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677526.1"
                     /translation="AAQSDRFGPVEFRAPDRPISVPTLRFPEAAGLSSDKISSTSTTG
                     MSAPENVLKQLDMEMGRTLSRSEPGRRGPPATTHDQRDERAQAKGTFK"
BASE COUNT         1288 a         1832 c         1970 g         1379 t
ORIGIN      
        1 ctgggtttct tggtcgcatg ataggtccag ttgatcccga accagatcac tccgagcagc
       61 agggctatga cgggaatggc gaccgccgga tgagcagccc agacggcgcc cacgaaggcg
      121 cgcatgtcac gccaacgcaa acggatgaag cgccacagct tcttacgggt aacgcggcgg
      181 cgcgactgcc ggcggcgatg tccgcggcga ttggaccggg gggacatgga aacatatcat
      241 agccgaatcg tgggaaaatg gatcaaggaa tgaaacatca caccctccag cttgtctgtg
      301 ctctcaggaa aacagagatc cagcaagcat aaagagcatg tgcaaccctg ctggtggtcc
      361 tacgcacgtc actcgcacct ttttcctatc cggtccgggc gagcgacctt cccgcacatc
      421 gggactccac agaagccagg ctgcgctttt tacattccaa ggtgcttctt aagaatcggt
      481 gaatccttga tggcgggatt cgcgggggcc agttccgccg ccttcttgaa ggaccccgtg
      541 gcgtcctcgt gcttccccat tttgtcgagt gagagagcca agttatagtg ggcttcggca
      601 aggttcgcat cggccttgat ggcttttttg aacccctctg acgccacatc ccaatgtccc
      661 tgcttgtaat ggccgacgcc ctcgtcattg atcgcacgac cgggtgaact ggcaggagcc
      721 atgagggcta tgtcagcacc cactgccgtc gttgccgtcc ccgcaggttt gccgtcatca
      781 cacgcgacca cggtgacgag acacgtaagc atcacactca gcgcaagaaa gacctgtttc
      841 acgttgaacc tcctaggtga catggatcag acaggtatcc gatcatgtct cattcaggat
      901 gtgcggtctg atcaatagtg ctcactcacg catcctcatc ccccattgcg ttccggccgc
      961 cattgaaggc ccaaggtgat gatctgctga agcagctgct tgtagggcac gcccgcctgt
     1021 ttggcggact cggcgaaatc ctcgccctcc gcgatttggg gattggggtt cgcttccagc
     1081 acatagagtt tctcgtctgt atccatccgt aggtcgatcc gcgcataccc gctcaggtcc
     1141 aaggaacggt agactcgctt cgcgagatgt tggatgcgct cgacgacgct gtcggacgcg
     1201 tccttcagtt ccgcggtctg gatgccatat ttctcctgat acttgttgct ccacttcacc
     1261 cgctccgtgg cgatccgatg gacgttgtcc ggcatcttgc taaattgcat ctcccacacg
     1321 ggaaagaccc gcagtcgttg gttgcccatc acaccgacgt agagctcgcg gccctcgatg
     1381 aattgttcga cgagtgcatc cgtgccgacg ctctcatgaa tgaatccaac ccgttcgcgc
     1441 aacttctgct catcctctac gacggacgcc tgggagatcc cgagcgagga ttcctcggtc
     1501 agcgatttga cgatcagggg gaaggcgagg cgcttgggca ggcggatctt cagcccccga
     1561 tgaaagatgg cgaactccgg caccggaatg cgatggtacg ccatgagttt cttggagagt
     1621 cccttgtctt tggcaatcag caggccacgt ggattacagc cggtgtaggg aatgcgtagt
     1681 aattcgaggt agctcaccac gttcggttcg aacaggctca cgttgtgaaa cgcttccagc
     1741 agattgaaga caatatgtgg cttccaggta tcgatggtgc tgcggatgat gccgagatcc
     1801 tgttccaccc ccaacggctc tgcctcatgg ccaatcgctc gtagtgtcgt tacgacgtca
     1861 aattctgttt tccattccgc cttctcagta tcgacatcgg acgtattctg aggcggtacc
     1921 aaatgtttgt gcatgagcac caggactttg agcggcttca tagcgccacc cgccggcccc
     1981 cggtgtggac gtagttcatc gcttgcactg tcaggaagac ggtaaattct tgtttagcct
     2041 gctcttccgg tactgccagg cgcagattga gcggacggca gtaatcgatc agatcttcga
     2101 agacttgatc gatcgtgtat tgatacacac ccgtccagcc ggcgacgaga cggcgaaccg
     2161 gcttccgaat cttcatcagg aagcggatgg ccgtcatgtt gcctgcgtgc tctgtcgcct
     2221 ccgagaagaa ccgctgcagg tcacgggcat tgaagtccgg atggtcgaca ccgtaatgcc
     2281 ggcgcttctt cttgtaatgc cggcgcaggg tggtgcgcag ccgccggagc ggatcaattt
     2341 cttccgtgga gacgttaagc ggaggtttac caaccagcgt ctgcatgagc tgatcgacgt
     2401 attgaagttt cttgagcgcc ggccatccgg catagcgtgt ctcccagagc gagttcgggg
     2461 tgagccagac ggcgaaggtt tcggcgaagt cctcatcggg atggctctgg gcgtaccagg
     2521 agtcgagatg aagcacgaaa ctcttgctat agggctttgg agaataatca tccggatacg
     2581 gctcggagct atttccgaaa agctgctgtc gatgccgtcg gcgtcgcagc ttgaaggcat
     2641 tctcgatcgc atggccggcc tcatgccgca ggatgcgcat gcatgaatcc ggatcgcctc
     2701 cttctacttc taacatttga gcgagttcga gcttggcgag gcgggggtgg gcgaggtaga
     2761 acgggatcgc gatgccgggc acatcatcag gacaaaacca ttcatctgac agccaaaaat
     2821 ggggatgaaa gcgaaggccg cggtcatcaa gttcttggta cagctgcgcg atgcggtcct
     2881 caagattgct acccgcaatc ttgacatcaa ggtcgcacag ccgaagatcc agcaacctct
     2941 catccggcca cgtcgcccaa tcggtgagat cgtttcgtct ggtatcgggg gacacatctg
     3001 gtgccggctc gctctcgtct tgtatggcca tcgttcaggt caacacagca tgagtcccaa
     3061 agccgggaca ttgtacgttt tgaccgatag tccagtcgat ccccaccggt gggccttgcc
     3121 agttcagtcg ttcggaggaa aaaaccaacg gctggaccta tccttcacat gtgggtcatg
     3181 tatgcgctca taggcatatg agcatgggct gttcagaatg ccaaggcgtt tgtcgattca
     3241 tgaaaaatag tcagcccctc ccttgttgac gtgcgcccgg ggggagcgta gggtccgccg
     3301 taggtgaggc cgactgaaca gcctgcggga tagtggggag ccatgcgcca tgctgaggtg
     3361 gttcgcgctg cgcacgagtg tgatcgtgct gatggccggc tgtatgtcgc ccggtgcctc
     3421 aaatccgcgg cacgacgtga cggcagtgtc gtatagaaca gatgcaacca agggactggt
     3481 cgtgctctcg gcgctgtggg gccgctcgtg gaagtgcggg acattcgagc acggacagtt
     3541 acgagccttt ggcttcgatc ggttgccgag tgtgactgag gcagaggatg cgacggtcga
     3601 tgtggtcatt gaagactcga cagaccttag agggggaccg gccgccaact acgtgctggc
     3661 attggagccc ggcgagtatg gtctggcgat gttcgccatc cgagtggcct cctctacgac
     3721 ggatatgaga accgtgagcg tcggacgtgc aaggctgttc gagaagggcg agccggtcgg
     3781 cgggacgttc acggtgaagg cgggtgagct ggtctacatc gggcacttcg gattggattg
     3841 ctctaaagag ccgacgattt ggcgctacta tttagatggg cgtggaggat tcgagcacta
     3901 caagcgcctg atcaaggacc aatttccatt tctgcccgtg gaggccatgc aaatgcgttt
     3961 gttcaagaca tccacattgg gacgggacta tgtccagcca caataatcga ggagtctttg
     4021 cttgaaggca gcagagttgc tggtccggtg cctggagaac gaaggcgtgg atcttgtctt
     4081 cgggttgcca ggcgaggaaa cgctcgagct cacggatgcg ctactcggtt cccgcacccg
     4141 cttcatcgag gcccggcacg agcagggtgc ggcatttatg gccgacgtgt atggcaggct
     4201 cagtggaaag gcaggagtgt gcctgtccac gctcggaccc ggcgcgacga atctgttgac
     4261 gggcgtcgcg gatgcattcc tggatcgagc gccgctggtg gccatcacgg gacaagcttc
     4321 gctgaaccgg cgccataagg aatcgcacca atacatcgat gtgatgtcaa tgttcaagcc
     4381 cgtcaccaaa tggagtgcgt cgataccgaa agcggaggtg attcccgagg ccgtgcggaa
     4441 agcattcaag atcgcgcaga cggagaaacc tggcgcgaca catttggagc tgcccgagga
     4501 tgtggcggag gagcagatcg gcgatgcgag ccagctgcag ccgttattcg tccaggcgcc
     4561 ggtcatgccg gagccgtcgc cggctcaggt cgcgagagcg gtccgcacga tttccggggc
     4621 gcagcgtcct gtgattctgg ccggaaacgg cgtgattcgt agccgcgcac atgaggcggt
     4681 cagacagttt gcgcgacgcg tgcacattcc cgtcctgcac acgttcatgg ccaaagggac
     4741 acttcccgac agcgagccgc tgtccctcta tacgattggc ctgttggcgc gcgattacac
     4801 gtcgactgtg atggagcagg ccgatgtggt gatcgcgatc ggctatgatt tcgtcgagta
     4861 tgccccctgc ttctggaatc cccatcgtga caagcgcatt gtgcacatcg acgggtcgcc
     4921 tgccgaggtg gacgagcact acattgtcga ggtgggtcta ctgggggacc tccgtcactc
     4981 cttagacctc atcggggccg cagttccgcc gttcgatttg tcctggtctt ccatatcacg
     5041 caagaccgtg ctcgacggat tcgaggcaga agtagccggc tcaccctcct ggccgctgcg
     5101 gccgcaacac ctgatgcggg aactccgcgc ggctctgagg ccggatgatc tggtcatctg
     5161 cgatgtgggt gcgcataagc tctggatggc ccgtatgttc ccctgcgagg tgcccaactc
     5221 ctgcatcatc tcgaacgggt ttgccgccat gggcattgcc gtgcccggcg ccattgccgc
     5281 aaagttatcg tttcccgaac gaagagtggt ggcggtcact ggcgatggtg ggttcttgat
     5341 gaactcccag gaactggaaa cggcggtgcg tctggcattg ccgctggttg ttctcgtgtg
     5401 gagggacaac ggctacggtg tgatccgctg gaagcaacag ctccggttcg gccgaacctc
     5461 cgccgtggaa ttcggcaacc ctgaccttgt gcagtacgcc aggagtttcg gcgcgatcgg
     5521 ctatcgcgtg gcggagccgt ctgagctccg gcctgtcttg gaggaagcgc tgaagagcaa
     5581 ggtgccggcc gtcatcgact gcccggtcga ctatgccgaa aatctccggc tgaccgaacg
     5641 actgcaagca ctcccgcact gaccctggtg ttccatgaga tgccgcttcc ttgttctcta
     5701 catgtcaagc acagcgcgcc tacgtcatgc ccaactcgaa cccaagacct gtcgcgacga
     5761 ggcatggttt tgtctttggg gccgccgaac gcccgtcagc ttgaggggag cagaaagtaa
     5821 atgatgacgg cgatcaccgg tccccctaca tagaacgctt tcttcccgaa ggcgtcccac
     5881 aagccgtacg ccaggatgcc ccagatcagc gtgtaataga tagacgcaat gccgcggtag
     5941 cctgcatgcg gggcctcttc agccaaggcc caatccggcc acgacagcaa gagcacaatc
     6001 aggagatgga acaggacttt catggagcct cctttgatct gcttttgaat ggtgaaagtt
     6061 gttcttcccg cctactgccc tgacgtcgtt caccgagaac cagggggatc gaggcgatca
     6121 gccagctcaa acagcccgcg cgtgtcgcga gtggagacca tccagatgtc gggggctgta
     6181 cacaggtggt tacttgaacg tgcccttcgc ctgcgctcgc tcgtcacgct ggtcgtgggt
     6241 cgtagccggc ggacctcgtc ggccgggttc ggatctgctc agcgttcgac ccatttccat
     6301 atcgagctgt ttcagtacgt tctcaggggc tgacatcccg gtagtggagg tactggagat
     6361 cttgtccgag gatagacccg cagcttcggg aaagcggagc gtcgggacgc tgatcggccg
     6421 atcgggagca cggaattcta cgggtccgaa tcgatcggac tgcgctgca
//
LOCUS       DASSCQ010000068         8905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_61450, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000068 DASSCQ010000000
VERSION     DASSCQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61450"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VFH05_04625"
     CDS             <1..1203
                     /locus_tag="VFH05_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6677527.1"
                     /translation="TDPSNALAYAEDVSSAQTANQRRVAYSGGLRFDYTWIPDSRHLV
                     KTGFQLDYTKANNQAQVFAFDTSSGAPAGPVLSLNASNQNIQTRQELWVQDQWSPTDD
                     WTFNLGVRGDAIQGFYNEGQISPRVGVTYKHNQSNVFHAYYGRLFTPPNVEQVAFAQL
                     NTQDTTAAPDNPTGFRPRAERSHYFEVGSYHALHRKATLELTGYYKLAHYLSDAGQFG
                     STPLLNFFAFERGWQRGIDGALKVQFADNFTGRGNVAWGQCKGYGLQSGQYLLDQAEI
                     DDINSTGGVFCDHSQFVTSSAVLSYRLWERTMFTGQMLYGSGLRTAEPGAKTNSSHEN
                     SYTVYNISVTHTISLPWHGQKMLLGFDVINLLDQHYFINRGEGSIGLGIAHAGMPRSY
                     FFRAQWFF"
     gene            1288..1740
                     /locus_tag="VFH05_04630"
     CDS             1288..1740
                     /locus_tag="VFH05_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6677528.1"
                     /translation="MTHTSSSSIGGKLCVILLAVWPVPVLAYATAGDEATHESDHQED
                     VLELPEVHVHGLPLNKDQQTGPVPKSTPWPNIPPALEGNVIDDWMKARMLVSKEAKVT
                     VVILEPCRYRELTAAGIAALAKWTFEPQMQGDVPVDGELTVRIHFHTK"
     gene            1790..2368
                     /locus_tag="VFH05_04635"
     CDS             1790..2368
                     /locus_tag="VFH05_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6677529.1"
                     /translation="MIWDVFFTRLINGLAGKHTWADWLFLTLSDPDMLWLPGILLGSY
                     WLWRSPREALIGGPILGLSIGILDFVGAQLKYLAARPRPCISMNDLVQLQACGKTFSF
                     PSNHAVNTATAAAFFQVLYPRSGWISWPIVGLIGIGRVYIGAHYVSDVIGGWMIGALC
                     GAGIAWLLLQGPRFRQRVLSPIVSTQADSSAQ"
     gene            complement(2314..3285)
                     /locus_tag="VFH05_04640"
     CDS             complement(2314..3285)
                     /locus_tag="VFH05_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET6677530.1"
                     /translation="MTQPAVPFYILCGSLGAGKTTLLMRLLEHWKRLGIRAGVLMNEA
                     GEISIDGPRAGTIAEQVMNLAGGCVCCDTKEDLSWGIAQLVREYKSELIVLECSGLAD
                     PAEVVDGVTDLYTARLARLERIIALLQPITNDQSPSSTFVTEQAIRYADELILNKRDL
                     YVPGHWEQFKASIVARNAHARVWETTHARIDAAALLSGHASLSKKEPTNVEFGRSRPP
                     SRLRAGYHPMATTVRLPGTLNRDRFLSWTKTLPKEIERAKGFLRFTQEPELQEFQYAP
                     PGEPAILPVMLLDEPEKAIVLIGRGYDVDRYCAELSACVETIGDNTR"
     gene            complement(3295..4233)
                     /locus_tag="VFH05_04645"
     CDS             complement(3295..4233)
                     /locus_tag="VFH05_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6677531.1"
                     /translation="MSLPVSYSRLQSRLRLALGLNAIVIVAEFVGGILINSIGLIGDA
                     GHNLIDQGALFAALYAHILTARPATEARTFGYHRAGIVTAFLSSFVLLLTALGITIVG
                     VQRLIHPVAVDGGGVMAIAAISFAANLAIALLLQHGAKDDLNIRSAFWHMLADAWVSL
                     GVILSGAAIVATGWAVFDPLISILLVGAIVRGAWPIFRESLEVLLESTPPGVSASHIA
                     ATIEAIPGVDNVHDLHVWAIEPRLVMLTCHVLVDADDTALTNELLRDIRSRITSDFGI
                     THVTIQLETHCAHKDAVHCDLMALAGRSANRLHAHP"
     gene            complement(4313..6271)
                     /locus_tag="VFH05_04650"
     CDS             complement(4313..6271)
                     /locus_tag="VFH05_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677532.1"
                     /translation="MMIPAERDKTPYSTLCVALSVLILITSVIAPLRLPFLLIPLSFF
                     LAAYGMGLLLEKAIPSIASSVSMLTLVVRLGTGISLLGCATAVLGLMGLYRLTALLVI
                     GALGWGLVHLGRSRGASNLLRPSCPSLISGVVLGIVWAIAWLWATIPPTFYDELAYHL
                     PLAQYALASDHVAAFPWLFFSFMPHLSDLLLGWGLALGGEIGARAMHIMFWLAIWLAA
                     WALVETIIVPRTCSWIGYLMAGAFASSSTFFFLGTLPFAETSLTFAVLAAAALIARPG
                     TFVFWLPLGLLWGLTVSVKLSGWMWVIAGALAALTMRWPAAALAKAGLVTLATTLPWW
                     GRAWWLTGNPIFPMGYRVLGGLHWSDESQARLQGDLPSYADPLNPVSLLKLPYDMVMV
                     PERFGSASDCGPLAVGMICVMVSLPLWTLVLNIDQERRRQCYAASLFIFFAGLCWIMT
                     STTTRFFAPALMVGLSTLVAMLTWLPKPALALSLIVLSACGTFGTARFLLLHDQVFSS
                     KHVALGQESAGEFARRTLDQYEAAMYVRSNLPPEAHLLLIGESRPFYFDRASLSPYPF
                     HEHPLTGWTREANSSKDLLDKIRREGFTHVILNTREFRRLNDGYHLFNFTGPEAMRQD
                     HILKQLPGNMTMLFSKNHVYVFEIPLSH"
     gene            complement(6316..7845)
                     /locus_tag="VFH05_04655"
     CDS             complement(6316..7845)
                     /locus_tag="VFH05_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6677533.1"
                     /translation="MIQQICLIIPPSLFLLDERVFMSLGILKVAAVLEQEDVTVELLD
                     LSGVMNYEEALAAHITHSQAECFGLTATTPQMPAATKIHATIRRLRPAARIILGGPHV
                     TLVYAAAKHERRRSVVGRASRAVKQLTAMFDVLVTGDGEIAVFEALQPAPPRIVDGDD
                     PKSPYFLSNASLEQMPFPARHLVDVSSYHYSIEGARALSMIAQLGCPFGCGFCGGRMS
                     PFLRRVRTRTSEHIVREMVHLYQTYGVTGFMLYDDELNVNPKITSLMSLIAHTQQQLG
                     VEFRLRGFIKAELFTDEQAACMYEAGFRWILIGFESGHHRILENIQKQASRDENTRCM
                     EIARRHNLKVKALMSLGHPGESEETIQTTRDWLVEVRPDDFDATVITTYPGTPYFDEA
                     VETSPGIWTYTCSKSEDRLHSVDVDYREVAEYYKGAPGNYTAFVYTDALTSEQLVSCR
                     DWLESDVRNTLSIPFNSADPGLRYEHTMGQSALPSTILRTSQLSSFRRHKALRVLPAG
                     G"
     gene            complement(7861..8604)
                     /locus_tag="VFH05_04660"
     CDS             complement(7861..8604)
                     /locus_tag="VFH05_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6677534.1"
                     /translation="MKISVIIPAFNEAHTIAAVIERVKSVALNGIQKEIIVVDDASTD
                     GTSDVLKDIRDIYAIRHDTNAGKGAALGTGIQAATGDVVIFQDADLEYAPEDYPVVIQ
                     PLIENRCDAVMGSRFLRGRPVFWGTRKSPYLNHYIGNLLIIWVTNFLYGKRFTDYEGC
                     YKAFRRSFLVDTPVESTGFEFDNELICKLMRKGVRIGEVPIQYHPRTYAQGKKITWRH
                     GLVMLWTILKWRFLPVPASQVVIDPNMAA"
     gene            8623..>8905
                     /locus_tag="VFH05_04665"
     CDS             8623..>8905
                     /locus_tag="VFH05_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677535.1"
                     /translation="MPRIVKGKQDCQGGQRWARCVEEEMNEFLSACRMKSFGNDGIVG
                     FANGKVAGGSIRRYDPKRNALRSLFIDGYEEGCVERPGVIRVKHLQAFDN"
BASE COUNT         2054 a         2413 c         2589 g         1849 t
ORIGIN      
        1 acggacccat ccaatgccct ggcctatgcc gaggacgtca gttcggctca gacggcgaat
       61 cagcggcgag tcgcctacag cgggggcctg cggttcgatt acacctggat ccccgacagc
      121 cggcatttgg tcaagactgg cttccagctg gactacacca aggccaacaa ccaggcgcaa
      181 gttttcgcct tcgatacgag cagcggcgcg ccggccgggc ccgtgttatc cctgaatgcc
      241 tcgaatcaga acatccaaac cagacaggaa ttgtgggtgc aggatcaatg gagtcccacg
      301 gatgattgga ccttcaatct cggggtgagg ggcgacgcca tccaggggtt ctacaatgaa
      361 ggccagatca gtccgcgggt cggtgtgacg tacaagcaca atcagtcgaa tgtcttccac
      421 gcctattacg gcaggctgtt tacgccgccg aacgtcgagc aggtggcgtt cgcgcaactg
      481 aatacccaag atacgactgc cgcgccggac aatccgaccg gttttcggcc tcgcgccgaa
      541 cgctcccatt actttgaagt cgggagctat catgctctgc atcggaaggc gacgctcgaa
      601 ttaaccggct actacaagct ggcccactat ctgtccgatg cgggccaatt cggcagcaca
      661 ccgctcttga atttcttcgc cttcgaacgg ggatggcaac gcggtatcga cggggcgtta
      721 aaagtgcaat tcgcggacaa ctttacgggg cgcgggaacg tcgcatgggg gcaatgcaaa
      781 ggctacgggt tgcagtccgg tcagtacctg ctcgatcagg cggagatcga cgacatcaac
      841 tcgaccggcg gtgtcttctg cgatcattcg caatttgtca ccagctcggc cgtgttgtct
      901 tatcgattat gggaacgcac gatgttcacc ggacaaatgc tctatgggtc cggcttgcgg
      961 acggcagagc ccggagcgaa aacgaactcg agccacgaaa attcctatac cgtttacaat
     1021 atctcggtta cccataccat ttcgttgcca tggcatggtc aaaaaatgct gctgggtttt
     1081 gacgtgatca acctgctcga tcagcactat ttcatcaacc ggggagaagg cagcatcgga
     1141 ctcggaattg cgcatgccgg gatgccccga tcgtatttct tccgcgcaca atggttcttt
     1201 tagattatga tgcagcccac tggaaggccg tttgagcatc ccacgcgaaa tactcttgac
     1261 catatgccaa ccaaacaagg tgccccggtg acacacactt caagttcgtc tatcggtggc
     1321 aagctctgcg tcatcttgct cgcagtctgg cccgtgccgg ttttagccta tgctacggcc
     1381 ggtgatgagg cgacccatga gagcgaccat caggaggacg tgttggagct accggaggtt
     1441 cacgtccacg ggttgccact caacaaggac cagcagaccg gaccggttcc gaaatccact
     1501 ccttggccca acattcctcc ggcactcgag gggaacgtga tcgatgattg gatgaaagcc
     1561 cggatgctcg tgtcgaaaga ggccaaggtc acggtcgtga tcctcgagcc ttgccgatat
     1621 cgggagctga ccgctgccgg catcgctgct ctcgcgaaat ggacctttga gcctcagatg
     1681 caaggcgatg tgcccgtcga cggagagcta accgtgcgca ttcacttcca tacgaagtag
     1741 gagccgtctt gccgttgacg aattctcgat cgccctatat ggtcagacga tgatttggga
     1801 cgtcttcttc acacgcttga tcaatggatt ggcgggaaaa catacttggg ccgattggtt
     1861 attcctgacc ttgtcagatc ccgacatgct gtggctgcct ggcatcctcc tcggcagcta
     1921 ttggctgtgg cggtctcccc gggaagcttt gatcggcggg ccgatcctcg gcctttccat
     1981 cgggatattg gacttcgtcg gcgctcaact aaagtatctg gccgctcgtc cacgtccttg
     2041 tatcagcatg aatgatctcg tccagttgca ggcttgtgga aaaacgttca gtttcccgtc
     2101 caaccatgcg gtcaataccg ccacggccgc ggcgtttttc caggtgctgt acccgcggtc
     2161 tgggtggatc agttggccca ttgtgggatt gatcggtatt ggtcgggtct atatcggcgc
     2221 gcactatgtg agtgatgtga tcggcggatg gatgatcggg gcgctttgcg gtgccggcat
     2281 cgcctggctg ttgctgcaag ggccacggtt ccgtcaacgg gtattgtcgc cgatcgtctc
     2341 gacgcaagct gatagctctg cgcaataacg atccacgtca tagcctcttc cgatcaggac
     2401 aatcgctttc tccggttcgt ccagcagcat gacgggtaaa atcgccggct cgccgggcgg
     2461 ggcgtactgg aattcctgca attcaggctc ctgtgtgaag cggagaaagc ctttggcccg
     2521 ctcgatctcc tttggcaatg ttttggtcca cgacaaaaag cgatcccggt tcaaggttcc
     2581 gggtaatcga accgttgtcg ccatcggatg atagcccgcg cgcaggcgcg aaggtggccg
     2641 cgatctgccg aactccacgt tggtgggttc cttcttggat aagctggcat gcccgcttaa
     2701 caaggcagcc gcatcaattc gcgcgtgggt ggtctcccac actcgcgcat gagcgttgcg
     2761 ggcaacgatc gacgccttga actgttccca gtggccgggt acgtacagat cccgcttatt
     2821 caaaatcaat tcatcggcgt agcgaatcgc ctgttcggtc acaaatgtgc tcgaagggct
     2881 ctggtcgttg gtgatcggct gaagcagcgc aatgatccgt tcaagcctgg ctagcctggc
     2941 cgtgtagaga tccgttaccc cgtcgaccac ttcggccgga tccgcgagtc cggaacattc
     3001 gagcacaatc agctcggatt tatactctct gacgagttgc gcgattcccc aggacaaatc
     3061 ttcttttgta tcgcaacaga cgcatcctcc cgcaagattc atgacctgct cggcaatggt
     3121 gccggcgcgc ggtccgtcga tgctgatttc gccggcctca ttcatgagca cgccggcccg
     3181 aatgcccagc cttttccaat gttcgagcag ccgcatcaag agcgtcgttt ttcctgcacc
     3241 aagcgatccg cagagaatgt agaaaggcac ggcaggctgt gtcatggtca caggttaggg
     3301 atgggcgtgg agacgattgg cggagcggcc ggccaatgcc atgaggtcgc aatggaccgc
     3361 atccttgtgc gcgcagtggg tttcgagctg aatagtcacg tgtgtgatgc cgaaatccga
     3421 agtaatgcgg gaacggatgt cacgcaacaa ctcgttagtg agcgcagtgt catccgcgtc
     3481 gaccagcaca tggcaggtca gcatgaccag tcggggttcg atcgcccaca catgcaagtc
     3541 gtggacgttg tcgacaccgg gaatcgcttc aatggtggcg gcgatatgag aggcgctgac
     3601 gccagggggc gttgactcca gcagcacctc cagtgattcg cgaaaaatcg gccaggcgcc
     3661 gcgaacaatc gctccaacca ggaggatgct gatcagcgga tcgaaaaccg cccaaccagt
     3721 cgccacaatg gccgctccgc tcaggatcac gccgagtgag acccacgcgt cggccagcat
     3781 gtgccaaaat gcactgcgaa tattgagatc atccttcgcg ccgtgctgaa gcaggagcgc
     3841 gatcgcgaga ttggcggcaa aactgatagc ggcgatggcc atgacgccgc ctccgtccac
     3901 ggcgacagga tggatgagcc gctgcacacc cacgatcgta atgcccaagg cggtcaacaa
     3961 gagaacgaac gagcttaaaa atgccgttac gatgccagct cgatggtagc caaacgtacg
     4021 agcttccgtg gccggtcttg ccgtcaggat atgcgcatag agggcggcga acagcgcacc
     4081 ctgatcaatg aggttatgac cggcgtcgcc gatcagtcca atgctgttga tgagaatccc
     4141 gccgacgaac tcagcgacaa tgacgatcgc attgagcccg agggcgaggc gcagtcggga
     4201 ttgtaagcgg gaataagaaa cgggaagact cattcgtata gtctcgcatg tgtttgaaat
     4261 ggcagcaaga gggttggctc catcagtctg ctcgtacagc ttgcccatct acctagtgtg
     4321 aaagaggaat ttcaaacaca tacacatgat tttttgagaa aagcatcgtc atgtttcccg
     4381 gcagttgctt gaggatatga tcctgccgca tggcctcagg tcccgtaaaa ttgaatagat
     4441 gatatccatc attgagtcgt cgaaactctc ttgtattcaa gatgacatgg gtaaagcctt
     4501 cacggcgtat cttgtccagc aggtccttgg aagaattcgc ttctctcgtc catccggtga
     4561 gcggatgctc atgaaagggg taaggggata acgatgcacg atcgaaatag aacggcctgg
     4621 attcgccgat caagagcaga tgcgcctcgg ggggaaggtt gcttctgaca tacatggctg
     4681 cctcgtattg gtcgagggtt cggcgcgcaa actcgccagc ggattcctgt ccaagcgcga
     4741 cgtgcttcga cgaaaagact tgatcgtgca ataggagaaa gcgagccgtt ccaaacgtgc
     4801 cgcatgcgga taggacgata agggataagg cgagcgcagg ttttggaagc catgtgagca
     4861 tagcgacaag cgtgctcagg ccaaccataa gcgccggggc aaaaaaccgt gtggtggtag
     4921 aagtcatgat ccagcagagt cctgcgaaga agataaagag acttgcggca tagcactgac
     4981 ggcgtctttc ttggtctatg ttcaatacca acgtccataa tggaaggctc accatgacac
     5041 aaatcattcc gacggcgagc ggaccgcaat cagaggcaga gccgaatcgc tcaggaacca
     5101 tgaccatgtc gtatggaagc ttcaataagc ttacaggatt caacggatca gcatacgatg
     5161 gtaagtcacc ctgcaagcga gcttggctct cgtcgctcca atgaagaccg ccgagcaccc
     5221 ggtagcccat gggaaagatc gggtttccgg tcaaccacca ggcccgtccc caccaaggca
     5281 gggtcgtcgc caatgtcaca agcccggcct tggccagcgc tgccgccggc catcgcatcg
     5341 tcagcgctgc aagagctcct gcgatgaccc acatccatcc tgagagtttg acagaaaccg
     5401 tcagtcccca gagcagacca agaggtagcc aaaacacgaa agttcctggc cgtgcaataa
     5461 gcgcagctgc ggcgagaact gcaaacgtca acgacgtctc tgcaaatgga agggtgccga
     5521 ggaagaaaaa tgtggaggac gaggcaaacg caccagccat taaatagccg atccatgaac
     5581 atgtacgtgg gacgatgatc gtttcgacga gcgcccatgc ggcaagccaa atcgccagcc
     5641 aaaacatgat atgcattgct ctggcaccga tttctccacc gagagccaaa ccccagccaa
     5701 ggagcagatc tgaaaggtgc ggcatgaagc taaaaaacag ccacggaaag gctgcgacgt
     5761 ggtcactggc aagcgcgtat tgtgcaaggg gcagatgata ggccagctca tcatagaatg
     5821 tcggaggaat cgttgcccag agccaggcta tggcccacac aatgccgaga acaacaccgc
     5881 tgatgaggga aggacaggat ggtctcaata agttcgatgc accgcgcgat cggcccaggt
     5941 gcacgagacc ccacccgagt gcaccgatga ccagtagcgc agtgagccgg tagagtccca
     6001 taagccccaa gacagcagtc gcacatccga gcaaagagat gcctgtgccg agacgcacaa
     6061 cgagagtgag catcgacaca gatgaggcga ttgacgggat cgccttttcg agcagcaatc
     6121 ccatcccata agctgccaag aagaaggata acggaatgag caggaaggga agccgaagcg
     6181 gggcgatgac agaggtgatc agaatgagca ccgacagtgc cacgcagagg gtcgagtagg
     6241 gcgtcttatc tcgctctgcg ggtatcatca tgtcatcgat cgggaggctc gattgctgaa
     6301 gagcggaaca gcatgttacc ccccggcggg caagacccta agagccttgt gacgtcggaa
     6361 cgaagagagc tgtgatgtgc gtagaattgt agagggaagg gcgctttgtc ccatggtatg
     6421 ttcgtagcgt agtcccgggt cggcgctgtt aaagggaatt gatagggtgt tgcgcacgtc
     6481 tgattccagc cagtcacgac aagacacgag ttgctcgctt gtgagggcat ccgtatagac
     6541 gaacgctgtg tagtttccag gagcaccctt gtaatattcg gcgacctccc gataatcgac
     6601 gtctacacta tggagccggt cctcggattt tgagcaggta taggtccaaa tgccaggcga
     6661 tgtctccacg gcttcgtcga aatacggcgt gcctggatag gtcgtgatga ccgtggcatc
     6721 gaaatcatcg ggacggactt cgacaagcca gtcacgtgtc gtctgtatcg tttcttcgga
     6781 ttccccggga tggcccaacg acatcaaggc tttcaccttc aaattgtgcc gtcgtgcgat
     6841 ttccatacag cgcgtgttct cgtcccggct ggcctgtttt tgaatgtttt ccaagattcg
     6901 atgatggcct gactcgaagc cgatcagaat ccatcgaaag ccggcttcat acatgcatgc
     6961 tgcctgctca tcggtaaaga gctccgcctt gataaaaccg cgcaggcgga attcgacacc
     7021 aagttgctgt tgggtgtgtg caataaggct catcaaagaa gtgatcttcg ggttcacatt
     7081 gagctcgtcg tcgtaaagca tgaagccagt cacaccgtag gtctggtaca gatgcaccat
     7141 ttcccgcacg atgtgttcgg atgtgcgcgt ccggacacgt cgaaggaacg gactcattct
     7201 tcccccgcaa aatccacagc cgaaggggca acccagttga gcgatcatgg agagcgctcg
     7261 tgctccctcg atgctgtagt gatagctgga aacgtcaacc agatggcgcg ccggaaatgg
     7321 catctgctcg agcgaagcat tgctcaggaa gtagggcgac ttagggtcgt cgccatccac
     7381 aatacgagga ggggcgggtt gcaaggcttc gaagacggct atttccccgt cgcctgtgac
     7441 gaggacgtcg aacatggccg tcagttgctt gacggctctc gaggcgcgac ccacgacact
     7501 tcgtcgccgc tcgtgctttg cagccgcata gacgagtgtc acatgagggc cacctagaat
     7561 aatccttgcc gccggccgaa ggcgcctgat ggttgcgtga atcttcgtcg cggccggcat
     7621 ttgcggcgtc gtggccgtaa ggccgaaaca ctcagcctgg ctgtgtgtga tatgggctgc
     7681 gagggcctct tcgtaattca tgacacccga cagatccagc agttcgaccg tgacatcttc
     7741 ttgttccaat accgcggcca ctttcaagat ccctagcgac atgaagaccc tctcgtcgag
     7801 aagaaagagc gacggtggga tgatgaggca gatttgctga atcatcggtg ctccgaccga
     7861 tcacgctgcc atgttagggt cgatgacgac ttgcgatgct ggaacaggaa ggaaccgcca
     7921 cttcaagatg gtccacaaca tcacgaggcc gtgacgccag gtgatttttt tgccctgcgc
     7981 gtaggtgcgg ggatggtatt ggatgggcac ttcgccgatt cgcacgcctt tccgcatcag
     8041 cttgcagatc agctcattgt cgaactcaaa gccggtcgat tcgacaggtg tgtcgacaag
     8101 gaaagaacgt cgaaacgctt tgtagcagcc ttcatagtcg gtaaaccgct tgccatacaa
     8161 gaagttcgtg acccagatga tgagaagatt gccgatgtag tgattgaggt agggggattt
     8221 gcgcgtgccc caaaatactg gccggccacg gagaaagcgt gatcccatca cggcatcgca
     8281 gcggttttcg atcagcggct gaatgacgac aggatagtct tcaggagcgt actcaaggtc
     8341 agcgtcctga aaaatgacca catcgccggt ggcggcttgg atgccggtgc ccaacgccgc
     8401 tcccttgccg gcatttgtgt catgacggat ggcgtagata tctcgaatgt ctttcagaac
     8461 atcgctcgtc ccgtcggtcg aggcgtcatc gacgacgatg atctcctttt ggatgccgtt
     8521 caacgcaacg gatttgaccc gctcgatcac ggcggcaatg gtatgtgctt cgttgaacgc
     8581 aggaatgatg accgaaattt tcacgaggtc aggatttcgt gaatgcctcg aattgtcaag
     8641 ggaaagcagg attgtcaagg aggacaacgc tgggcgcgtt gtgttgagga agagatgaac
     8701 gaattcttat cggcgtgccg gatgaagtcg ttcggaaacg acgggatagt gggcttcgcc
     8761 aacggaaaag ttgccggtgg cagtatccgc cgctacgacc cgaagcgtaa tgccctcagg
     8821 agcctcttta tcgacgggta cgaagaaggg tgtgtcgaac gtccgggtgt cattcgagta
     8881 aaacatttgc aagcgttcga caatg
//
LOCUS       DASSCQ010000069         4421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1589764, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000069 DASSCQ010000000
VERSION     DASSCQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589764"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFH05_04670"
     CDS             <1..666
                     /locus_tag="VFH05_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6677536.1"
                     /translation="RSVADGHLRLSKARKRRIADRMEKPAQAKHRIEDVIAHRWSPRA
                     FDERPVEPEKLKSLFEAARWAPSSNNEQPWRFLAANKEQGPDYDRLLSCLSEGNSKWV
                     FRAPLLILSVASLFFEDDGKPNRHAMHDTGLAVENLVLQATALGLQAHQMAGYDVEKA
                     RKACQIPSGFEPVAMIAVGYPGDPEILPEYLRERELKPRERQPIEDFVFTASWGRTSP
                     LVR"
     gene            798..1676
                     /locus_tag="VFH05_04675"
     CDS             798..1676
                     /locus_tag="VFH05_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6677537.1"
                     /translation="MADPKQRLDTNVQGNFFVDATCINCDTCRQLAPDSFQEVGECSA
                     VVIQPVGDEGLHRAYQALLACPVGSIGTQRSDKTRLQQAMSSFPLLIEGGVFYCGFNS
                     QKSFGANSYFIRHSDGNWLIDSPRYLKHLVDALERMGGLRYIFLTHEDDVADAARYAA
                     RFGATRIIHRADAEAMPDAEWIVDGSDSIPLAPQFNLIPVPGHTPGSSALLYDQQFLF
                     TGDHLWWDPETRSLQAPQRLVWRSRAMVQSLEKLLGDQFEWVLPGHGDRIKLTPEEMR
                     NQLRLLVDHRLAALAS"
     gene            1688..3322
                     /locus_tag="VFH05_04680"
     CDS             1688..3322
                     /locus_tag="VFH05_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677538.1"
                     /translation="MLLSIYHWIDRNVLALGREMRLSYLPPLMVYAAYGISGLTGIVG
                     TFFVKDYLSLSASFLAALGFWAGIPWALKMPIGHLVDLLWRWKSWLVFLGAGLLAVSL
                     GIMAALIGHREAMTAMFPAEVWFVLSALLAPIGYVIQDAVADAMTVEAVPRVNDRGQP
                     FEEQTRKLMHTTMQTLGRVAIIGGGIIVALINVSIFTGAEQLPQSEMVRLYERIYLMA
                     LAIPFISVLGIGLAWILQRRHRNRLRREGFTTDQADRMVKVRDDPSEPTHPNWWILGG
                     SVGFVAFILTVGLGNVPYSEEVVFAGSMAIVLFLMLRLTQELEPDKRFTLIGTAVVIF
                     IFRAIPGPGPGTTWWMIDELKFDQQFLSVLSLIGSTLTLLGMFVFRRFMAERSIAYVV
                     GFLTIVGTMLSLPILGMYYGLHEWTARVTGGVIDAQFIAVIDTALESPLGQISMIPML
                     AWIANSAPAHLKATFFAVMASFTNLALSLSQLGTKYLNEIYVVTREVRDPVAGTIHTP
                     ADYSQLGELLIVQLLLGLALPLAAILFARISRFKSA"
     gene            complement(3347..4066)
                     /locus_tag="VFH05_04685"
     CDS             complement(3347..4066)
                     /locus_tag="VFH05_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677539.1"
                     /translation="MPPIPIDITIGFILLPLVVLTTFSFLLVTRSRSPIWIVAVMGGW
                     AIFTGAMAFSGILAKFTSFPPWIPLLVLAQLAFVFWLGWASPWSKSLAEIPQYLLVGL
                     QCFRIPVELLLATLATRRLLAIEMTYYGRNFDVLEGITAVALAIWLKRCGENSLRHVV
                     LVWNLTGLSLVTVVLVHGLLSVPYPSQLLHLSVPTFIIAHFPVVWLLTVLVPMAYLLH
                     LVSIRRCLAKALASSETGQDS"
     gene            4215..>4421
                     /gene="ssrA"
                     /locus_tag="VFH05_04690"
     tmRNA           4215..>4421
                     /gene="ssrA"
                     /locus_tag="VFH05_04690"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          927 a         1248 c         1298 g          948 t
ORIGIN      
        1 cgctcggtgg cggatggtca cctgagattg agcaaagcca ggaaaagaag gatagctgat
       61 cgcatggaaa agccggcgca ggcgaagcac cgaattgaag acgtcattgc acatcgctgg
      121 agtcctcgtg cgttcgacga acgtccggtg gagccggaga aattgaaaag tctgttcgag
      181 gcggcgcgct gggcgccctc ttcgaataac gaacagccgt ggcggtttct cgctgcgaac
      241 aaagaacagg gtcccgatta cgacagattg ctcagttgtc tctctgaggg gaattcgaag
      301 tgggtatttc gtgcacccct attgatcctc tcggtcgcga gcctcttttt tgaagacgac
      361 ggaaaaccca accgtcatgc tatgcatgac accggcctag ctgtcgagaa cctcgtcctt
      421 caagccaccg cccttggatt gcaggcgcac cagatggccg gatacgatgt ggagaaggcg
      481 cgcaaagcct gtcagatccc ttctggattc gagcctgtgg ccatgattgc ggtaggatac
      541 cccggcgatc cagagattct gccggagtat ttgcgcgagc gcgagctgaa gccgcgagag
      601 cgtcaaccga tcgaggattt cgtgttcaca gccagctggg gccggacatc gcctctcgtg
      661 cggtgaaccg gtcgtccact cgtccccagc ctcgcgcatc tttcgaaggt tgaaggtcgg
      721 tcccgatacc atggtgtgcc cgcgcggcgg aagggcgatg cggaagcgaa cggctccgcg
      781 gatcgaggct tcatacaatg gcggatccaa aacaacgtct cgacacaaat gtccagggca
      841 acttttttgt cgatgcgact tgtatcaact gcgatacctg ccggcagctg gcaccggata
      901 gctttcagga ggtgggcgaa tgttccgccg tagtgatcca gcctgtgggc gatgaaggcc
      961 tgcatcgggc gtatcaggcg ttgctcgcct gtcctgtcgg ctcaatcggc acccagcgca
     1021 gcgataagac gcggttacag caggcgatgt ccagctttcc cttgctcatc gaaggaggcg
     1081 tgttttattg cgggtttaat tctcagaagt ccttcggcgc caacagttat ttcatccgcc
     1141 attccgacgg gaactggttg atcgattcgc ctcgctacct gaaacatctg gtcgatgccc
     1201 tcgagcgtat ggggggcctc cgatatattt tcctcaccca tgaagacgat gtcgccgatg
     1261 ccgcccgata tgctgctcgg ttcggcgcaa cccgcatcat ccatcgtgcc gacgccgaag
     1321 caatgccgga tgcggaatgg atcgtcgatg gttcagacag catcccgttg gctccgcagt
     1381 tcaatctcat ccccgtgccc ggacacacgc cgggcagtag tgcgttgctc tatgaccaac
     1441 aatttctgtt caccggcgac catctctggt gggatcccga aacccggtcg ctgcaagcgc
     1501 ctcagcggct cgtctggcga tcgcgtgcca tggtgcagtc cctcgaaaag ctgctcggcg
     1561 accagttcga atgggtgttg cccggacacg gcgatcgtat caagcttact ccggaggaga
     1621 tgcgaaacca gcttcgcttg ctggtcgatc atcgtcttgc cgcccttgcc tcctgatccc
     1681 atccctgatg ctcctatcga tataccactg gattgaccgc aacgtcctgg cgctgggacg
     1741 tgagatgcgc ctgtcgtacc tcccgccgct catggtctac gcggcctacg gtatttcagg
     1801 tttgaccgga attgtcggga cgttctttgt caaagactat ctgagtcttt ccgcttcgtt
     1861 cctcgccgcg ctcggattct gggccggcat tccctgggca cttaaaatgc cgattggcca
     1921 tctcgtcgat ctgctgtggc ggtggaaaag ttggctcgtg ttcttgggcg cgggactgct
     1981 tgccgtcagt ctggggatca tggcggccct gatcggccat cgggaagcca tgacggcaat
     2041 gtttccggct gaagtatggt ttgttctgag cgccctcctg gcccccattg ggtatgtcat
     2101 tcaagatgcc gtagccgacg cgatgacggt tgaagcggtt cctcgagtga atgatcgtgg
     2161 tcagccgttt gaggagcaga ccaggaagct catgcatacc accatgcaga cactcgggcg
     2221 tgtggcgatc atcggcggcg gcatcatcgt ggcgctgatc aacgtgtcca tcttcacggg
     2281 tgctgaacaa ctgccgcaaa gtgagatggt gcggctctat gaacgaatct atctcatggc
     2341 gctggcgatt ccattcattt cggtcctcgg catcggattg gcgtggatac tccagcgccg
     2401 tcacaggaat agactgcggc gcgagggatt cacgaccgat caggcggatc ggatggtcaa
     2461 ggtgcgcgat gatccgagcg aaccgacaca tcccaactgg tggattctcg gcgggagtgt
     2521 cgggttcgtc gccttcatac tcaccgtcgg attgggcaac gttccctata gcgaagaggt
     2581 cgtctttgcc gggtccatgg ccatcgtgct gtttctcatg ctcaggctga cacaggaact
     2641 ggagcccgac aagcgattca ccttgattgg aacagcggtg gtcatcttca tctttcgtgc
     2701 cattccgggc cccggaccgg gtacgacctg gtggatgatt gacgagttga aattcgatca
     2761 acagtttctt tccgtgttat cgttgatcgg gagtacgtta accttgctcg gcatgtttgt
     2821 ctttcgccgc ttcatggccg aacggtcgat cgcctatgtc gtcggctttc tcacaatcgt
     2881 cggtacgatg ctctcgcttc ccattctcgg gatgtattac ggcttgcacg aatggacagc
     2941 gcgtgtcacc ggcggagtca tcgatgcgca atttatcgcc gtgatcgata ccgccctcga
     3001 atcaccgctc gggcagatct ccatgattcc gatgctggcg tggatcgcca attctgctcc
     3061 ggctcactta aaggcgacgt tctttgccgt gatggcctcc tttacgaacc tggccctgtc
     3121 cttgagccag ctcgggacga aatatctcaa cgagatctat gtcgtcaccc gggaggttag
     3181 ggatccggtg gcgggcacca tccacacacc cgccgactac agtcagctcg gcgagttact
     3241 gatcgttcag ctgctcctgg gccttgccct gcctcttgcc gcgatcctgt tcgcacggat
     3301 cagccgcttc aagagcgcct aaagccggag cgtaaggcgg tctcgttcaa gaatcctggc
     3361 ctgtttcgga agaggccaaa gccttggcta aacaacgcct gatggagacc agatgaagaa
     3421 gataggccat cgggacgagg acggtgagca gccagacgac ggggaagtgc gcaatgataa
     3481 acgtgggaac ggagaggtga agaagctgtg acggataggg aaccgacagc aatccatgca
     3541 cgagcacgac cgtcacgaga ctcaagccag tcaggttcca aacgagcacg acatgccgca
     3601 gagagttctc gccgcagcgc ttcagccaaa tcgccaaggc gacagccgtg attccttcta
     3661 ggacatcaaa gtttcttccg tagtacgtca tttcaatcgc aagcagcctc ctcgtggcca
     3721 gcgtggcgag aaggagttcg accggaatgc gaaagcattg cagaccgacg agtaggtatt
     3781 gtggaatctc cgccaaggat ttgctccacg gtgacgccca accgagccaa aatacgaagg
     3841 caagttgggc caggacaagg aggggaatcc agggcggaaa cgaagtgaac ttcgccaata
     3901 ttccggagaa cgccatcgcc cccgtgaaga tcgcccaacc ccccatgact gccacgatcc
     3961 agatcggcga cctcgaccgt gtaacgagaa gaaagctaaa agtggttaaa acgaccaatg
     4021 gaaggaggat aaagccgatc gtgatgtcga tcggtatcgg cggcatggcc cagagcttac
     4081 cacagtcccg aggacttcac gcctgaccta gcatcctctt gacccatgcg atatcatgac
     4141 catctaagat gagggctacg agtctcggag caagtgagag ggcagtcaga agccctttga
     4201 acattctcaa gaaagggggc gaccggtttc gacggggata ctgataccac cgtggcatgt
     4261 cgagctctcg gggactcgtc aactccggga aaatgcaact gccaatcaag aactggcact
     4321 cgcagcttaa ttaagctgtg acgttcttcc atctgaggcc cgcgggggtg gaagggcgcg
     4381 atgcagcggg ctggtccaag gctggtgccc agcggctgag g
//
LOCUS       DASSCQ010000070         6884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_4446, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000070 DASSCQ010000000
VERSION     DASSCQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4446"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..360)
                     /locus_tag="VFH05_04695"
     CDS             complement(67..360)
                     /locus_tag="VFH05_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677540.1"
                     /translation="MKICPTMKRLALLSGVTFMLVATLSPVDAEDRLVEGAMILAPVE
                     GGSRMGNIFDDTLEACLARIPERVTPEQLRLAHQVCHEEEATRELEPLGVKGD"
     gene            complement(872..1114)
                     /locus_tag="VFH05_04700"
     CDS             complement(872..1114)
                     /locus_tag="VFH05_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677541.1"
                     /translation="MNQEQFGQFWLQLRTPLKAKWEKITTEDLDEIQGDLAKFGAVLQ
                     RRYGEMHKEEVTTWANRRYSHWTGNYAGYKDPEPAS"
     gene            complement(1464..1988)
                     /locus_tag="VFH05_04705"
     CDS             complement(1464..1988)
                     /locus_tag="VFH05_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET6677542.1"
                     /translation="MARDVFVAVLVATFLTTASLESAQKLDDKETAFLQKAAQGQLAE
                     IALGTLALKKASHKDVKEFGAEISEDHQYASQEVKELSASEGIYLPVELDAEHKKQQQ
                     RLSRLSGKAFDEAFLASILKNHRKDLRDLEKNAAVLHNAKVKQWVESTLPILTVHLKK
                     AERVAEAIGLGQAE"
     gene            2493..3617
                     /locus_tag="VFH05_04710"
     CDS             2493..3617
                     /locus_tag="VFH05_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6677543.1"
                     /translation="MKIAQIAPLWESVPPKLYGGTERIVSYLTEELVRQGHDVTLFAS
                     GDSQTLARLWASSPQAFRSQSEMVNRDVPLVLMQEQVFGVEAGRFDIIHSHLDFLGFP
                     LSRRCATPVLTTLHGRLDLPELVPMYREYADMPVVSISNAQRLPLPNANWQGTVYHGL
                     PDLYTFHPNPGSYLAYLGRICPEKRPDHAIEIAKRVGIPLRMAAKIDPHDREYFETKI
                     APLLDHPLVEYLGEVTDAEKCDLLGNAVAVLCTYDWPEPFGIVLIEALACGTPVLAYR
                     CGSIPEIIEDGVTGFICDNLDGMVAAIDRLPLIRRQRCRDSFKTRFTVERMVQDYLGL
                     YQHMAASVRPIVRMNGRSSHLLDHRRTPPKDLVLPHIGTA"
     gene            4067..4387
                     /gene="erpA"
                     /locus_tag="VFH05_04715"
     CDS             4067..4387
                     /gene="erpA"
                     /locus_tag="VFH05_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET6677544.1"
                     /translation="MITMTPTAEQKIRELLVEEKDTIGLRVFVKGGGCHGYQYGMAFE
                     SQLGEDDTVIEKGDVKVIMDSQSAPLLAGCEVDFQDTVQGSGFAIKNPQAKTTCGCGS
                     SFSA"
     gene            4424..5632
                     /locus_tag="VFH05_04720"
     CDS             4424..5632
                     /locus_tag="VFH05_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET6677545.1"
                     /translation="METVSIIGTKNRKGQVITDPRVMMNEAPRTASFFTGSEVIKEAV
                     RRANVDIMVAYPITPQSEAAALCGELFAEGYIGDYFRGESEFAVMSQCAGSAFGGARV
                     FTTTAGPGTMRAMENFPMWAGARLPIQCIVTCRGINSPLSIQPDTIEIAYLMNTGMLV
                     WHAETAQDFYDWILKGYMVSEEPDVHLPLALCCDGFFVTHTKDTVDLTPTDMCLPPYD
                     PYRSPVPCMDMECPPVRMMRDPFVMKSNYISYATHASWQQEIWAAAERSRKHTIAWLD
                     GLIDTENIDAEVLIVTSGTAVSQGREAIRLLADEGIRVGLVKIKTLRPWPEAELLEAT
                     KHAKHIIVPEFNVIGWMAREIKATIPRPERVIAGPRVCGGMTMPPEIIVEEIKKAIGV
                     RSEVLAGRGS"
     gene            5715..6605
                     /locus_tag="VFH05_04725"
     CDS             5715..6605
                     /locus_tag="VFH05_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6677546.1"
                     /translation="MSLDYVKFSPGFEAYMPKEYRDMVEHGPFGKKTTVSQMGSFKEI
                     LEEHPMCAGCAMTLFIRLAIIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRIRFGDQPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTTRGAVLKMAPLGKKFEKMDMVAMAKIAGCAYIATVVPNNPRRVESCI
                     KKAVLIAREVGPTYIQAYTSCNIEYAIPTDQVMADAKTVEDDRYKFAEYVSDEAREYL
                     AARYGYKEFNRKPAAAIGTT"
BASE COUNT         1539 a         1962 c         1864 g         1519 t
ORIGIN      
        1 gaggcccaga tcgctatccc ccacgtcatc accaatatcg ccaacaggct gtatatttga
       61 tacatctcag tctcctttga ctccgagtgg ttctaattct ctcgtcgcct cttcctcatg
      121 gcagacctgg tgagccagcc taagctgctc tggagtgacc cgttctggaa tccgcgctaa
      181 acaggcttcc agcgtgtcat caaaaatgtt tcccatgcgg ctgccccctt caaccggagc
      241 cagaatcatg gctccctcga cgagcctatc ttccgcatca acaggcgaca gggtcgctac
      301 caacatgaag gtcacgcccg aaagcaacgc gagccgtttc atcgtggggc agattttcat
      361 ttcatacttc cttccttcgg attaattcga tgaagcggac gcacttccgt catcgaatga
      421 gacaatttga acgcgatgtg gcaaaacaca gattccagga ggagcggcgg agactggaga
      481 atcagatcgc gctgggaagg tgagagtggc gcgaaagata gggcagtcgg cgtagctcaa
      541 tacatggttt caccctacag cccagcatcc cagtagtcaa atcccagaca gtgcgtttgc
      601 acgctaacaa actgatacag cacaggtata attatttcct taggacgatc tttatgacac
      661 ccgcgtgacg aaagtaatcg gcttctgctc tatttgcttc gctgggtcga tgcgctcctc
      721 tcccgtcgat tccctgccga gagtgcatct tccacttgcg tattcaccgc gcggtcttct
      781 gaaacggctt tgcagacttt cacatctgcc aacctcagca gtccatacca tcgtgagaac
      841 gacgtagaga gtgagcgctt acgacaagtg gttatgacgc aggctcagga tctttgtaac
      901 cggcataatt tcccgtccaa tgagaatacc ggcggttggc ccaggtggtc acttcctctt
      961 tgtgcatctc gccatatcgc ctttgcagca ccgcgccgaa cttggcaaga tcgccctgga
     1021 tctcgtcgag gtcctccgtc gtaatcttct cccacttcgc tttcagggga gtcctcagtt
     1081 gtaaccaaaa ctgcccgaat tgttcctgat tcataccgtg ctccttggtt agtgatggac
     1141 gtctcctcgt atagaccact cgacaggtgt gaagcgctgg aaatcaaacg cgtgaagaaa
     1201 aatgagggag gcgcgaaaga tacctgcatc ttactctgaa gcgaggggaa tcaaaacggc
     1261 ggatgaggcg aaccaccccg taagactacg aatccacaca catatattta ttttatgcgt
     1321 gacctggata cctcgatctg tcctgacttg tccatctacc cgacgtcgcg acacttcgtt
     1381 gcaggattta ccacatcacc cactaacccc aagtggcgct tctcttccga tacacctcgt
     1441 caccgcgaca gtatgcgcag ttgctactcg gcctggccga gtccaattgc ctctgccacc
     1501 ctttccgcct tcttgaggtg aaccgtcaga atcggcagtg tgctctccac ccattgcttc
     1561 accttcgcgt tatgtagcac cgccgcgttc ttctcaaggt ccctgaggtc ctttcgatga
     1621 ttcttcagta tagaggcgag aaatgcttcg tcgaatgctt ttccggacaa acgggacaat
     1681 cgctgctgtt gttttttatg ctctgcgtcg agctcaacag gaagatagat cccttcactg
     1741 gctgagagtt ctttgacttc ctgactcgca tactggtggt cttcactaat ctccgcgccg
     1801 aattccttca catccttgtg agaagccttc ttgagggcaa gcgtaccgag tgcaatttcc
     1861 gccaactgtc cctgggcagc cttttgcagg aacgccgttt ccttgtcatc cagtttttga
     1921 gcggattcga gcgaagccgt ggtgagaaag gtggcgacga gtactgcgac aaacacgtca
     1981 cgagccatgg aacctccgtg ggatttatgg ttggcaactc tctgtactat ggtccagccc
     2041 gtccacgcca ggaactagga atcgcctgag agtaagcgaa ttctaaccgg accaaatagc
     2101 ggtcatggcg ccaggaggct gtgttggcgc ggaagagtac cgtttgagga cgcacacaaa
     2161 aatttataat gctgtagaac cattcgctta taaggtcagg acgaagcgga gccatggtgc
     2221 ccgcctatct ttcactgata tgatggcttc attatcaggg aaatgtttcg ttgagctacg
     2281 ccgaccgtct catctctcgc gcccactccg ctctcgcatc gcgcgtctga ttccgcaggt
     2341 ctacgctgcc tgagttgcca caacgcattt cctccaggct tgtcgtgtga aagaccacga
     2401 cagcttaaag gataagaccc actacgttta tcaggactct gttagatcac ggctaatcac
     2461 tcagcttttc gttcggaaaa gaaaggccgc acatgaaaat cgcacagatc gcccctcttt
     2521 gggaaagcgt cccccctaag ctatacggag gcacggagcg aatcgtctct taccttacgg
     2581 aagaactggt tcgtcagggg catgacgtca ccctctttgc aagcggggat tctcaaacgc
     2641 tggcccgcct ctgggcctcc agccctcagg cgtttcgatc gcaatctgag atggtcaatc
     2701 gtgatgtgcc actggtactc atgcaggagc aggtcttcgg tgtagaggca ggcagattcg
     2761 atatcattca ttcccatcta gactttttgg gcttccccct gagtcgccgt tgcgcgaccc
     2821 cggtactcac gactttacac ggacgattgg acctaccaga attagtaccc atgtatagag
     2881 agtatgccga catgccggtt gtgtcgattt caaacgcgca gcgcctgccc ttgccgaacg
     2941 ccaactggca gggcacggtg tatcacgggc tgccggacct ctacaccttt catcctaatc
     3001 ctggatccta tctcgcctat ctcggaagaa tctgtccgga gaaacggccg gatcacgcga
     3061 tagaaatcgc caaacgggtt ggcattccgc tccgaatggc cgccaagatc gatccacacg
     3121 atcgtgagta ctttgagacg aagattgcgc cgctgttgga ccatccactc gtggaatatc
     3181 tcggtgaagt gacggatgca gaaaagtgcg atctactcgg gaacgcggtc gcagtcctgt
     3241 gcacgtatga ctggccggaa ccgttcggca tcgtgttgat tgaggcactg gcttgcggaa
     3301 cgccagtgtt ggcctaccga tgcgggtcga tccccgagat cattgaagac ggtgtgacgg
     3361 gatttatttg tgacaatctg gatgggatgg tggcggcgat tgaccggctg ccgctcattc
     3421 gacgccaacg atgtcgggac tcgttcaaga cccgtttcac cgtggagcgc atggtccaag
     3481 actatctcgg cctctatcaa cacatggccg cgagcgtgcg accaatagtc cgcatgaatg
     3541 ggcggtcatc tcatctgttg gatcacaggc ggacgccccc gaaggacctc gtcttgcctc
     3601 acatcggtac cgcgtgacct ctgtgagcac aggctattga gggaacagct ctcgagcatg
     3661 cgagagccta tgagagtccc gtgctcgctt gctccgcgtt tgctccgtga cgtcagaaat
     3721 tatggcgttg tgagtatgct acagcggcga cgcattgtgg atggcgatcg cctggaccgt
     3781 ttaaccatac gactacccct taatagcgct tgactccggt acctatagcg cctagcctga
     3841 tcttagacgt ataaagccac gccgactgtc ctaatccttc gcgcgtcttg cctcacctct
     3901 tgcgcgactg attccccggt cctcgccgct tcctacgcca acgtttcttt cacactcctc
     3961 gtgtgaatgg aggcggtgcc gcttctgatc cgagaagagc gccgccatat gccaatcggc
     4021 ttaatgtttg cgcccaaggt ccgccaacga caaaaggaga gatgccatga tcaccatgac
     4081 gccaacggct gaacaaaaga ttcgggaact cctcgtggaa gagaaagata cgatcggcct
     4141 gcgcgtcttc gtcaaaggcg gcggctgcca tggctaccaa tacggaatgg ccttcgagtc
     4201 tcagctcgga gaggacgata cggtcatcga aaagggcgac gtcaaagtca ttatggattc
     4261 gcaaagcgct ccgctgctgg ccggctgcga agtcgatttt caagacaccg tgcaagggtc
     4321 cggctttgcc attaaaaatc cccaggcgaa aaccacgtgt gggtgcggca gttcattcag
     4381 cgcatgactc aacgctgcgc aggaagctaa gggaggttga tccatggaaa ctgtgtcaat
     4441 catcggaacg aaaaatcgaa aaggccaggt catcaccgac cctcgcgtca tgatgaacga
     4501 ggcgcctcgc acggcgtcgt tcttcaccgg cagcgaagtc atcaaagaag ccgtcaggcg
     4561 cgccaacgtc gatattatgg tggcctatcc cattacgccc cagagcgaag cggccgctct
     4621 ctgcggcgaa ctctttgccg agggatatat cggcgattac ttccgcggtg aaagtgaatt
     4681 tgccgtcatg tcgcaatgcg ccggctccgc atttgggggg gcccgcgtct tcaccaccac
     4741 cgccggccct ggaaccatgc gcgccatgga gaacttcccg atgtgggccg gggcgcgctt
     4801 gccgatccaa tgcatcgtca cttgccgagg catcaattct cccctctcga tccaaccgga
     4861 cacgatcgag atcgcctatt tgatgaacac gggtatgctc gtctggcacg ctgagaccgc
     4921 gcaggacttt tacgattgga ttctcaaagg ctacatggtc tcggaagagc cggatgtgca
     4981 tctgcccttg gccctctgtt gcgatggatt cttcgtgacc catacgaaag ataccgttga
     5041 cctcacccct accgacatgt gcctgccgcc ctatgatccg taccggtcgc ccgtcccctg
     5101 catggacatg gaatgtcccc cggtccgcat gatgcgtgat ccgttcgtca tgaagagcaa
     5161 ttacatcagc tatgccacgc acgcgagctg gcaacaggaa atctgggccg cggccgaacg
     5221 gtcccggaag catacgatcg cctggctgga cggactgatc gacaccgaaa acatcgatgc
     5281 cgaggtgctc atcgtgacat cgggaaccgc tgtttcccaa ggccgtgagg ccatccgtct
     5341 gttggccgat gaaggaattc gtgtggggct ggtgaagatc aagacccttc gcccttggcc
     5401 ggaggcagaa ctcctggaag ccaccaagca tgccaaacat atcatcgtgc cggagttcaa
     5461 tgtcatcggg tggatggcac gggagatcaa agccacgatc ccccgtcccg aacgggtcat
     5521 cgccggacca cgcgtctgtg ggggcatgac catgccgccg gaaatcatcg tagaagaaat
     5581 caagaaagca atcggtgtcc gatccgaggt tctggccgga cgcggcagtt gaaacggcgg
     5641 cgggccagcc ggcgagatac aaaccattgt tcattggcgc gattgcgccg agccacaaga
     5701 gaggacggta tccaatgagt ctcgattatg tgaagttttc accaggattt gaagcgtaca
     5761 tgccaaaaga atatcgcgat atggtggagc acgggccatt cgggaaaaag accacggtgt
     5821 cgcaaatggg aagtttcaag gaaattcttg aggaacaccc gatgtgcgcc ggctgcgcca
     5881 tgaccctgtt catccggttg gccattatcg ccttcccgaa tcccgaagac accattacgg
     5941 tcgggacggc cggatgcggc cggttggcca tttcgcaggc agccattccc ttcgtctacg
     6001 gcaactatgg cgaccaaaac ggcgtcgcca gcggcttgtc gcgtggattg aggatccggt
     6061 ttggggatca gcccaaagat gtcgtcgtga tggccggtga cggcggcacg gccgacattg
     6121 gatttcagca ggtgctccat tcctggttcc gaaaggagcg gttcacgaca atcatgctgg
     6181 ataacgaagt gtacgggaac accggcgggc aggaaagcgg catgaccaca agaggcgcgg
     6241 tgctcaagat ggcgccgctc ggaaagaaat tcgagaagat ggatatggtg gccatggcca
     6301 aaatcgccgg ctgtgcttat atcgccaccg tcgtgcccaa taatccgcgg cgggtggaga
     6361 gctgcattaa gaaggcggtg ctgatcgccc gagaggttgg tcccacgtat attcaagcct
     6421 acacgtcctg caacatcgaa tatgcgattc cgaccgacca ggtgatggcg gacgccaaga
     6481 ccgtcgagga tgatcgctac aaattcgccg aatatgtcag cgatgaggcc agggaatatc
     6541 tggcggcgcg ttacggatac aaggagttta accggaagcc ggcggcagcg attggaacga
     6601 cgtgaacctg acgtaaggct cgtgcgctca gctcagccgt gagtcatttc gaatcgccgg
     6661 agcgtccgag tgcggcgctg tcatcaagct cattgctcgg tcacggctga gtcggagatg
     6721 caaatggtct tggcggacca tggaaagcag gctgtgtaac catgcagacg ctcgacatcc
     6781 atcgtcccga gatgcccgat cttcagttcg tgctgttggt gacggcactg tgcacttccc
     6841 ggctgaccgc gctcaacata ccgtacacac tccgcaccgc aata
//
LOCUS       DASSCQ010000071         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1074382, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000071 DASSCQ010000000
VERSION     DASSCQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074382"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VFH05_04730"
     CDS             complement(<1..1349)
                     /locus_tag="VFH05_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6677547.1"
                     /translation="MGIAGKILIVDDEEDALENCRRILSRVPYACVSTSNPSHALALL
                     EREHPGLVLTDLRMPKMDGIEVLTAAKRADPNVQVVLVTAHATIETAVTSMRYGAFDY
                     ITKPFTSEELIQVVRRAFDAEGGDGGGAHRPAASPPRTGVSTHDGPVQIVGSSGLMRA
                     VLSLADRVAPTDSNVLIYGESGTGKEMLARYIHQMSRRVDRPFVPVDCVSLNDALLES
                     ELFGHEKGAFTGAHAAKSGLFEVAHGGTLFLDEVSGMSPNLQARLLRVLQERQVRRVG
                     GTRFTHVDVRVIGASNENLEDLCRKGTFREDLYYRLNVIPVALPPLRSREGDVLLLAN
                     EFVTRFVQRVQTVPVSVPVIDPSAADLLMRYAWPGNVRELQNVMERASVLVDGPTVMS
                     THLPDRLQALADRDSVPAESASFKQAKQQAVESFERNFLIDLLKRNEGHMSNAAREAG
                     "
     gene            complement(1325..>3265)
                     /locus_tag="VFH05_04735"
     CDS             complement(1325..>3265)
                     /locus_tag="VFH05_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677548.1"
                     /translation="VSGMQSLRDVIGGNLQAVAVQAADRVTMLVQGEVRNARLLASAP
                     LRVRLPVQVANSDYPSDPEGARLLIGHRTLLWEQGGDHAASLLNGELSRFLLETKIRD
                     GDKVIGLMILDRHGALVAASSEPDKYFFGNESWWTLLGTGGDQLYLSGRIPAEEGAFR
                     ASDETLDIAVPILDDRQQQMIGAVIASYRFDSLFAMIAQIQIGQTGHAMLFDAAGQPL
                     VCPILARDAHRIPSQLMAMIVSSDPGWAVAEDDGHGASNTVVGYAPVNGLQLPDNSWH
                     IFVRQHPSESYAPIHDQVRNLAAIGLVMLGLLGAMGRYVATRIARPIQLLHSGVEAIS
                     QGTYDQPINIKTGDEFEDLSAAVHRMADRLKASRAELEGLNQDLARRVEEKTQEITKH
                     MQSLELAERLASLGKVASGIAHEINNPLGIILNRIECMEADAVRLPLSEELGRDLMTI
                     RSHAERISRVTKSILTFSRGTVSVLKPIDMNGIVRSCLVLAGERISALSVHLESSLTH
                     SLPSVAGDRDRLETVLLNLINNAIDAVSMRGSEGTVWVQTSMVEMEGQQWVKISVADN
                     GPGIPSANIDRIFDPFFSTKTAGQGTGLGLFLTYGIVSDHRGRIEAKNGKTGAIFTVV
                     LPTVGASNRTSRGASWESQAKS"
BASE COUNT          671 a         1015 c          910 g          669 t
ORIGIN      
        1 ccggcttcac gggctgcgtt gctcatatgg ccttcgttgc gtttcagcag gtcgataagg
       61 aaattgcgct caaaggactc gaccgcctgc tgcttggcct gcttgaacga cgcggattct
      121 gcaggcaccg aatctctgtc agccagcgcc tgcaaccgat cgggcaaatg ggtgctcatg
      181 actgtgggac catccaccag cacggacgcc cgttccatga cattttggag ctcccggacg
      241 ttgcccggcc aggcatagcg catgagcaga tccgccgccg aaggatcgat gacgggaacc
      301 gagacaggaa ctgtctgaac ccgctgcaca aaacgcgtca cgaattcgtt ggccaacagt
      361 aaaacatccc cttctctgga ccggagagga ggaagtgcca ccggaatgac gttcagccga
      421 taatacagat cctcgcgaaa tgtccccttc cggcacaaat cttcgagatt ttcattcgag
      481 gctccgatca cccggacatc gacgtgcgtg aatcgcgttc cgcccacccg acggacttgc
      541 cgttcttgca agactcgcaa cagccgagct tgaaggttgg gactcatgcc gcttacttcg
      601 tccaggaata gtgttccccc atgtgccacc tcgaagaggc cggattttgc ggcatgtgct
      661 ccggtgaagg ctcccttttc atgaccaaac agctcagact ccagcaaggc atcgttcagg
      721 gagacacagt ccacaggcac gaaaggccgg tccacccggc ggctcatctg gtggatatac
      781 cgagccagca tttcctttcc ggttccactc tcgccgtaga tgaggacgtt ggagtccgtg
      841 ggtgccaccc ggtccgcgag ggataagacg gccctcatga gcccactgct gccgacgatc
      901 tgcacaggac catcgtgggt ggacactccc gtccgcggcg gcgacgccgc cggccgatgt
      961 gctccccctc cgtcgccccc ctccgcatcg aatgcgcggc gcacaacctg aatcaattct
     1021 tcactggtga acggtttcgt gatgtagtcg aatgcgccgt agcgcatgga agtgacggca
     1081 gtctcgatcg tggcatgcgc cgtgaccagc accacctgca cgttcggatc cgctcgcttg
     1141 gccgcggtca acacttcgat gccgtccatt ttcggcatac gcaagtcagt caacacaagg
     1201 ccaggatgtt cacgctcaag cagcgccagg gcgtggctag gattggaggt cgagacacag
     1261 gcatagggca cacggctcag aatgcgccgg caattttcca gggcgtcttc ctcgtcatcc
     1321 acgatcagga ttttgcctgc gattcccatg atgctcctcg tgaggtgcgg ttcgacgcgc
     1381 ccaccgtcgg cagtaccacc gtaaagatcg cccccgtctt cccatttttc gcttcgatac
     1441 ggcctcgatg atccgacacg attccatagg tcagaaacaa cccgagccct gttccttgtc
     1501 cggccgtttt ggtgctgaag aacggatcga agatgcgatc aatgttcgcg ctcggtatcc
     1561 ccggaccatt gtcagccacc gagattttta cccactgctg cccttccatc tcgaccatgg
     1621 atgtctgcac ccagaccgtt ccttccgagc cgcgcatgct gacggcgtca atggcgttat
     1681 tgatcagatt gagcaagacc gtttccagcc ggtctcgatc gcccgccaca gacggcagtg
     1741 aatgagtcag tgacgattcc aggtgaacgg agagagcaga gatccgttct cccgccagga
     1801 cgagacagct ccggacgatc ccattcatat cgatcggctt cagcacggac accgttccgc
     1861 gcgagaacgt caggatgctt ttggtgacac gagagatgcg ttcggcatgg gaacggatgg
     1921 tcatcaaatc acgccccagt tcctctgaca gcggtaatcg caccgcatcg gcttccatgc
     1981 attcaatgcg attgagaatg atgcccaacg ggttattgat ttcatgcgca atgccgctcg
     2041 cgaccttgcc caatgacgcc aggcgttcag cgagttccaa actttgcatg tgcttcgtaa
     2101 tttcctgtgt tttttcctca acccgccgag cgagatcctg attgaggcct tcgagttctg
     2161 cgcgggaggc cttcagccta tcggccatgc ggtggacggc cgcggagaga tcttcaaact
     2221 catccccggt cttgatattg atcggctggt cgtacgtacc ctggctgatc gcttccaccc
     2281 cgctgtgcaa caattggatg gggcgggcga tgcgggtcgc gacatagcgg cccatggccc
     2341 ccaagaggcc gagcatcacc agaccgatgg cagctagatt cctcacctgg tcgtgaatcg
     2401 gcgcatagct ttcactcgga tgttgcctga cgaagatgtg ccaggaattg tctggcagtt
     2461 gaagcccgtt caccggagcg taccccacta cggtgttgga ggcgccatgc ccgtcgtcct
     2521 ctgccacggc ccagcccgga tcggaggaca cgatcatggc catcagctga ctggggatac
     2581 gatgggcatc gcgcgcgaga atcggacaaa ccaacggttg gcccgccgca tcaaagagca
     2641 tcgcgtggcc cgtttggccg atctggatct gggcaatcat ggcaaataga ctgtcaaaac
     2701 ggtacgacgc gatgactgcc ccgatcatct gctgctgccg gtcgtcaaga atgggaaccg
     2761 caatatcgag cgtttcatcg gaggctctga atgccccctc ttccgcggga attcgcccgc
     2821 tgagatacag ctggtccccc cctgtgccca aaagggtcca ccaagactcg ttcccgaaaa
     2881 aatatttgtc cggctcggaa ctggctgcca ccagcgctcc atgtcggtcc agaatcatga
     2941 gtccgatcac tttgtcgccg tcgcggatct tggtttcgag gagaaaccgt gagagttcgc
     3001 cattcaacag ggaagcggca tggtctccgc cttgttccca gagcagggtc cggtggccga
     3061 tcaagagcct cgcgccttcc ggatcggaag gatagtccga gttggccact tgaacaggta
     3121 agcgcacacg taatggcgca gaggccaaca gcctggcatt gcgtacctcc ccttgcacca
     3181 acatcgtcac acgatcggcc gcttgaacgg ccacggcctg caggttgccg ccgatcacat
     3241 cccgcagcga ttgcatgccg gagac
//
LOCUS       DASSCQ010000072        23241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1609368, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000072 DASSCQ010000000
VERSION     DASSCQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 23241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23241
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609368"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="ruvB"
                     /locus_tag="VFH05_04740"
     CDS             <1..828
                     /gene="ruvB"
                     /locus_tag="VFH05_04740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET6677549.1"
                     /translation="GPPGLGKTTIAHIIALEMGGTFCATSGLVLTHAGDLAAILTNLQ
                     EHDVLFIDEIHRLPAAVEEALYPAMEDFQLDLVVGQGPAARTVKLDLPRFTLVGATTK
                     AGSLTSPLRDRFGLVHRLEFYSPAELEVIVMRSARLLGIPIDQEGAAEIACRARGTPR
                     IVNRLIKRVRDYAQVKAQGEITRAVAQEALAWLGVDAAGFDEMDRHILLTIIDKFGGG
                     PVGVESLAAAVQEDRSTLEDVHEPYLIQAGFLDRTGRGRQVTKAALDHFKRPSSLLY"
     gene            1002..2078
                     /gene="pheA"
                     /locus_tag="VFH05_04745"
     CDS             1002..2078
                     /gene="pheA"
                     /locus_tag="VFH05_04745"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET6677550.1"
                     /translation="MPGDLSDYRKEIDRIDDEILRLLNERSKSVVQIGRLKMLQDAGA
                     NLHTPAREAAIIERLVAQNTGPFPSDAIRPVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACTQKFGASAQYVPVNSIKEVFSEVERGRANFGVVPIENTTEGVVNHTLDMFIDSN
                     LLIYGEVLQEVSHHLMSKSGAAEDIKKICSHPHAIAQCRNWLETNMPHVPVSEVASTA
                     RAAEMCLDDSSIAAIASELASQLYGLKVIKSRIEDNLNNFTRFLVLSQKPPQRTGKDK
                     TSLMLSIKDKVGALYDLLRPFASHGLSMTKIESRPSRRKAWEYIFFVDIEGHIEEERV
                     KKAVEEITGRCLFMKVLGSYPAHS"
     gene            2083..3174
                     /gene="hisC"
                     /locus_tag="VFH05_04750"
     CDS             2083..3174
                     /gene="hisC"
                     /locus_tag="VFH05_04750"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET6677551.1"
                     /translation="MTLHVHPDIAALSPYVPGKPIEELQRELGLSRVIKLASNENPLG
                     PSPKALAVINECAATLHRYPDGGAFKLRQALAHRWKVTDEQIILGNGSDEILGLLART
                     FLSPGDEAVMADHTFVIYHMEVMAAHGKAVTVPLKDWRHDVAAMASAITARTRLVFLC
                     NPNNPTGTMVSAEEVAWLLGRVPDHVVVVFDEAYFEYVRSSEFPDSMAWIKEGRHVIV
                     LRTFSKIYGLAGLRIGYGVTTGEIANLLNRVRPPFNANSIAQRAALAALDDDEHVARS
                     RAVNQAGMEQIATGLRALGLAPINSEANFVYVDVGRDGRYVFDAMLRHGVIVRHIEGR
                     MIRVTIGQAEENRVFLAALAQVLQQGEQG"
     gene            3182..4195
                     /gene="aroF"
                     /locus_tag="VFH05_04755"
     CDS             3182..4195
                     /gene="aroF"
                     /locus_tag="VFH05_04755"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6677552.1"
                     /translation="MIIVLKPEASEREVDHIVDRLRELGLKSQLSTGQERTIIGVIGD
                     DRILQNQPLTALPGVESVLPILAPWKLVSREFKKEGTIIDVGGVKIGGNKLAIMAGPC
                     AVERLELTVGIAHEVKAAGASVLRGGAYKPRTSPYSFQGLGREGLDYLAEARKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNRNVMLCERGIRTFETQYRNTLDLAAIPTLKELSHLPVIVDPSHATGK
                     WNLVAPMSKAAVAAGADGILIEVHSNPECALCDGEESIKPLKFKELMHDMRKIAIAVG
                     REV"
     gene            4195..5076
                     /locus_tag="VFH05_04760"
     CDS             4195..5076
                     /locus_tag="VFH05_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET6677553.1"
                     /translation="MARLFRQAAIIGVGLIGGSLGMILRRKGLASSVVGVGRRIENLK
                     LAVKLGAIDHYVVDAKEGVKDADVVVLATPVDTYQRHLEEWASCLKSGAIVSDVGSVK
                     GELVEQCERLMPDGVHFVGAHPIAGKEKTGVAAGSEDLFSGMRCIVTPTKQTNPQALE
                     RVRTLWQEAGSTVLTMDPHLHDKILGAVSHLPHVVAFALMGALIDIRTEVPSLDLAGH
                     SGGGLRDTTRIAASSPEMWRDIFLWNRDNVVAFIEAYERSLGELKQLIRAGDAAGIEK
                     ALERAKQEREKFPVRTG"
     gene            5102..6415
                     /gene="aroA"
                     /locus_tag="VFH05_04765"
     CDS             5102..6415
                     /gene="aroA"
                     /locus_tag="VFH05_04765"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET6677554.1"
                     /translation="MKTLTITPGRPLRGTIAVPGDKSITHRAIILTALAEGMGRIKRY
                     CRGEDCLNTMRALQALGVRIDEADDELCVHGKGLWGLNEPAVVLDCGNSGTGIRLLTG
                     LLAGQDFFSVLTGDASIRRRPMGRIVKPLREMGATIAGRKGGELAPLAVTGTTLRALT
                     YTSPVASAQIKSSLLFAALFAKGTSRFSEPRLSRDHTERLFQFYGLPLTRDGLTLTME
                     GRPSVGWTAAAELTVPGDFSAAAFFIVGGTIVPDSDVTITGVGINPTRTGLLEILQRM
                     GGDIQVENYRDEAGEPVADIRVRSAPLHGVTIGPEWIPQSIDEFPILCVAAAVASGQT
                     IVTGAQELRVKESDRIATMGAELRAMGAQMNEKADGMVIEGLGHARGNGRLRGTDGKS
                     HGDHRVAMSIAIAGLTALDETRVHDADCIETSFPDFDQVLTQLTS"
     gene            6436..7095
                     /gene="cmk"
                     /locus_tag="VFH05_04770"
     CDS             6436..7095
                     /gene="cmk"
                     /locus_tag="VFH05_04770"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET6677555.1"
                     /translation="MIVAIDGPAGVGKSTVAKLLASRLGFLYLDTGALYRSVGLAVLH
                     HGIQPHDETAVAGLLPKLSLEIEVANHTVHVSLNGKDVTGELRTPAVSAAASIVSAIP
                     DVRAWLLPIQRQIGRKGSVVAEGRDVGTKVFPAAPVKFFLEADPTVRADRRHRELVAA
                     GHSRAIEQTSAELAGRDNRDRSRSAAPLVAADDAHHIDTSRLSAEEVVERMLAVVTAK
                     L"
     gene            7092..7742
                     /locus_tag="VFH05_04775"
     CDS             7092..7742
                     /locus_tag="VFH05_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6677556.1"
                     /translation="MSSVAYGILWVLARAIAWLCFRFRVEGAVPKQGGVLVAANHASY
                     FDIPLLGCGMFRRAWYLGRSDLFIPGLKVILRWLGWIPLKLGRLDRKAIDQAIALIKA
                     GKVVVIFPEGGRSLDGRLRQAKAGLGMIVAQTGCPVVPAYLKGTFDVLPAGAKRPRFH
                     PVSVSFGPPLSFSTTGNGDGKQFYQNVSRVVMERIAALGGVAPPPHHASAGARNAE"
     gene            7801..9513
                     /locus_tag="VFH05_04780"
     CDS             7801..9513
                     /locus_tag="VFH05_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET6677557.1"
                     /translation="MSTVSKTNEPQLDREALAALYEETFKNLEEGTITEGRVVALAKD
                     KVVVDIGYKSEGMIPSDQFSHDDLQQLKIGDRIQVYIEECEDADGNLILSKEKADKMK
                     IWEELEKLYKDEKSIDGKVVSRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGRTF
                     PMKIIKINHRRGNVVVSRRVLLEETRDKKRQTTLATLKEGQLIQGMVKNITDYGAFID
                     LGGIDGLLHITDMSWGRVGHPSEMFNVGDRVEVSVLKYDRETGRISLGLKQKSADPWT
                     NVAGKYPIGTRVKGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSRVVAIGD
                     QVEAAVLNVDPASRKISLGMKQTAPNPWDMVEGKYPLGTRIEGKVKSLTDFGAFIGLD
                     EGIDGLIHISDMSWTKHIKHPSEIFKKGQKVEAVVLRIDKEKERLSLGYKQLSRDPWD
                     DEIPNRYRVGDPAVGKVSKVADFGIFVELDGGVEGLIHVSESGLDQSAKLEEKFKLQD
                     EVTAKIIKVDREERKIALSLRDHQLDSDRRHVDEYHATQGAPDQSLGRAAKQSRKRGA
                     DDDK"
     gene            9573..10469
                     /gene="sppA"
                     /locus_tag="VFH05_04785"
     CDS             9573..10469
                     /gene="sppA"
                     /locus_tag="VFH05_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET6677558.1"
                     /translation="MEQETTGKPPKRSWLRRILLVAGIGIALLLLLNVLFPDLDLSTE
                     DRVALIRVEGVILDAQSTVTELKRFGENPSIKAIVLRIDSPGGGVVPSQEIYDAVQRV
                     RNKHNKAVIASMGTVAASGGYYIAAATDRIIANPGTLTGSIGVIMETANVEGLLKKIG
                     VEGIVVKSGKFKDVGSPLRKMTEEEQKLLQSVMDDVHQQFIDAVAEGRALEPTAVQAL
                     ADGRIFTGRQAKEAKLVDELGNLDDAIQLAADLAGIEGEPKVIEPRRRFSIREFLESR
                     FSALFPKLDFYSGVSLKYLMAF"
     gene            10519..10797
                     /locus_tag="VFH05_04790"
     CDS             10519..10797
                     /locus_tag="VFH05_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6677559.1"
                     /translation="MTKAQIIERVSEQVTTLTKRQAEIVVNTIFDCVRDSLKNGDKTE
                     IRGFGSFRLRSRRMKEGRNPKTGATVAVPAKKVPFFKAGKELKELLNQ"
     gene            10818..11819
                     /locus_tag="VFH05_04795"
     CDS             10818..11819
                     /locus_tag="VFH05_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HET6677560.1"
                     /translation="MAASDSNDTATAEVRWTEGALKRMERAPIFLRGMVRRLAEKKAR
                     ELGYAEITEATLEQFKSQMMGGMGGASGMAQAAEDMAQGRLPWTAAAKERLNTVPEFM
                     RGMIRQIAEEVAREGGHMEVNVDLFERVEAMGEVREAAAPPLAWTEGALARLQDKLKQ
                     SPPIAVEFVTDMLKRDTEDLAREKGLTQIDEQVLIQLWDAPQERVAWTDEAWKRLQTS
                     PDFVRSGIRKAAERRARKLGLKEIDSEHLTGFRNQAMMKAVKRIRSFGYNELTFEAFD
                     TALEKTKRLQGNEQAEKRLQEIRSHFADPDTKKPEGGTLGAELMGRFRRYLKGEGSL"
     gene            complement(11983..12303)
                     /locus_tag="VFH05_04800"
     CDS             complement(11983..12303)
                     /locus_tag="VFH05_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677561.1"
                     /translation="MGKTVKIDTTMYGIAEVLHWCHDRNKGRIPGVDTAGFEKMKALL
                     AEKPQSGDYFTLDQFWKKKVALELTEEEVALIDRCLYDIPNLDNEPLPQIRHKFWPQQ
                     IAAH"
     gene            12468..13694
                     /locus_tag="VFH05_04805"
     CDS             12468..13694
                     /locus_tag="VFH05_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET6677562.1"
                     /translation="MDGREAVLREMRGFTTTWSCRCLISGLLVVMSGACSKQDPYVPP
                     DLFYYFASYKVGKNPTTITPFDINQDGVTDLMTTNVSSNTLSLLIGNGDGTFHDHVQL
                     HVCQEPRSLATGAFNADSYPDIALACAGGDEVAVLFGRADGKFQEGPQYPVHRTPVAI
                     ASDDLNGDGMADLVVALRNDKIKVFLGQGNGEFRHGAQYEYGDTPTSVALSDLNGDGK
                     TDLVVTNGGPMSNAVSIWLGNGDGSFRSPTDYRSGRRPLGVSVADFNNDRIRDLLVIN
                     GEHDSFSIFLGNGNATFQAAHDSGANAGPNFGLARDFNGDHRTDVAIVNLQSSDLSIL
                     FGRGDGTFEYPPRNYRTKAGPFALALYRVTSTEVEEPGLVTADNGDGSVSIFLHRGLK
                     AGARPKTAASSSQSTQ"
     gene            13837..14010
                     /locus_tag="VFH05_04810"
     CDS             13837..14010
                     /locus_tag="VFH05_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677563.1"
                     /translation="MGALLTLSVLMVQGGVRDLFDGSHPSGGASPYVSFATTIFGGGL
                     LAGCLALVMKRIR"
     gene            14031..14219
                     /locus_tag="VFH05_04815"
     CDS             14031..14219
                     /locus_tag="VFH05_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677564.1"
                     /translation="MDCLKCKGFMMVERHYTLMNRRLYARCLNCGFWIDLADLLRFFH
                     KVIDSGFRGSKVRESFTL"
     gene            14240..15127
                     /locus_tag="VFH05_04820"
     CDS             14240..15127
                     /locus_tag="VFH05_04820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET6677565.1"
                     /translation="MFSLTQRTSTFFLAALCIVLLAPSTVLAKRGNGKTRHVTAISED
                     HHHLPWRRVPAHSILLKELNSGRVLYEHDSAKRLSPASLTKIMSALVILEHGRLDDLV
                     TVSPAAARAHKTHLRLKAGQVFKLDDLLKAMLIVSANDACLAAVEHVGGDETQFVGLM
                     NDKAAALGLHNTHFSNGCGFDGPEHYSTAEDLATLSIIALMQPIFRDLVKEERAIIMP
                     VNGNHPYILHTTNRLLGRIPGVEGIKTGFTSKAGRCLIAKVSQNGSDLLLVILNSSRR
                     WNTATSLITYGLRASGPSL"
     gene            complement(15206..15739)
                     /locus_tag="VFH05_04825"
     CDS             complement(15206..15739)
                     /locus_tag="VFH05_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677566.1"
                     /translation="MEALIRMGVRSCLVIGIIGLVVASALAEPYELSKNDVMDPKAIK
                     SPDISLFGVKIGDTESKAMDVLVNEKIPGIKVEQEALFIFLVDQRKPTGPMAGVRVQD
                     GKVDLIFINNRFAYKTRGLFRNVLNSESPEDIRKLLGQEDYGDENVMGAVLAYDKQGF
                     VINYLGKDVNIEFSPVH"
     gene            complement(15879..16172)
                     /locus_tag="VFH05_04830"
     CDS             complement(15879..16172)
                     /locus_tag="VFH05_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677567.1"
                     /translation="MDRDTVGRLWEAHKQEEWPRVASQVEGPLMTLDTVISGCVVYFL
                     DSEDGLDEQRRTIVGDCLADLEAMEQELDQECRAYFQRLRELGTLLLIAGRSS"
     gene            complement(16224..17489)
                     /locus_tag="VFH05_04835"
     CDS             complement(16224..17489)
                     /locus_tag="VFH05_04835"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET6677568.1"
                     /translation="MSSLHELDTPTFRLAVAQFDEAAELMGLDANLRERLKFPQRSLV
                     VSVPIRMDDNRVEVYTGYRVQHDSSRGPSKGGIRYHPDVNLGEVAALAMWMTWKCALA
                     DLPYGGAKGGVAVAPKQLSRGELQRLTRRYAAEIFPLIGPEKDVPAPDVGTDAQVMAW
                     IMDTYSQQVGYAVPGVVTGKPLSIGGSLGREEATGRGVVCVIQEALKHLRIEMGHTTV
                     AIQGFGNVGSHTARIMYESGAKVVAVSDVEGGIYNAGGLDIPTLLAKYKGTGQTLADT
                     KLGDRITNDELLQLNCTVLVPAALSEQITDRNAPKLNCRVLAEGANGPTTIGADRILH
                     DKGIFVIPDILANSGGVIVSYFEWVQDAQRFFWKATDIQERLNDLMTSAFQRTLQFAT
                     TKRATMRIAALMIGIDKVAKAHLHRGLYP"
     gene            complement(17489..18283)
                     /gene="lipB"
                     /locus_tag="VFH05_04840"
     CDS             complement(17489..18283)
                     /gene="lipB"
                     /locus_tag="VFH05_04840"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET6677569.1"
                     /translation="MTAIDFPAGAQILDPSIKTPLPQGELITISKPVQYGIAWRLQQR
                     LHSDRVAGRRVDTLVLLQHLPVYTVGRRTKPGHVRLHLSSDHDDVPIESVTRGGSVTY
                     HGPGQLFVYPILALSRYAPGPKTYVRMLEEVLLRTLARLNVNGYRLTRTPGVWTGSSC
                     GEAKIASIGARVDLGVTLHGFALNVDLDLSPFSRIVPCGLDGCRMTSMAEIMGAPVSL
                     ELVGRHVAESFSSVFQLEWRCPYPGTTMPPSIGEEPMISPSGEDFR"
     gene            complement(18280..19317)
                     /locus_tag="VFH05_04845"
     CDS             complement(18280..19317)
                     /locus_tag="VFH05_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6677570.1"
                     /translation="MATLPLILIPVASLLFGSSGAWANPGSSAVVSTDKTLSVPAVVA
                     KVRSSVVTILTRGVPASPSQVQSGSGSGSGVIIDEGGYILTNNHLVTGVKSMVVGLST
                     GRLTPGRVVARDFLLDLALVKINAQDLVPAVLNPRPDLEIGESVVAIGNPLALKGGST
                     VTVGVVSALDRSVLTPDGETLYDLIQTDAAINPGNSGGPLVDLSGRVVGINVIIAPSA
                     QAISYALSMEAIYPHIQSMMVRGSIYRPDLGFTPVTITPSVMASFGLDGDRGVLALQV
                     DAAKPAGVGGLQNGDIITAVDQHQVFNMGDFWHALLRSGDQPTVQLTLHGRSGPATIQ
                     LPKPAALKAAQ"
     gene            complement(19432..20685)
                     /locus_tag="VFH05_04850"
     CDS             complement(19432..20685)
                     /locus_tag="VFH05_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6677571.1"
                     /translation="MKNDLFSADGPELKSPELKDADTDHSEDSDLLDMIGRNSTEVES
                     VQEAPKPIKRQIESSSPFLLESLYFQSFGERALLTREEETALAKRIDQGSTMIRATLL
                     HVHRLLASVKRTQTITEVQQTLQTIRRLSGLSATALDKVERSLLDLIKGEDGFKVPAT
                     VIKALKSDLARVRQARQVLEKAKDELVRCNLRLVVDVAKHYTRRGLTLLDLVQEGNIG
                     LMKAAERYQYRKGFKFSTYATWWIRQGITRALADQSRTIRIPVHQTEASHRILRVTRR
                     LGQQLGRPAKLEEIANVLRMRPERLHETVQAFQEPVALEHPVGDGGTEFGELLPDLQA
                     VSPDAHVHRTEMTHQLDRILENLTPREQTVIRLRFGIGYDQPSTLEQVGQNLSVTRER
                     IRQIEAKALKKLKTPAIKEMFAAIQ"
     gene            21054..21947
                     /locus_tag="VFH05_04855"
     CDS             21054..21947
                     /locus_tag="VFH05_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HET6677572.1"
                     /translation="MSSTTLQNGDRIHLVDRKGRQYALTLKAGDIYQISGHKIAHDDL
                     INKLDGSVVHLSGGKSMLAMRPTFGEYVLKMPRGAQVLYPKDLALMTMWADVYPGARV
                     FEAGTGSGALTMALLRAIGPRGVVVTYEAREDFARTARSNIERYVGPVPNLIALRKNA
                     YEGIDLLEDGVPFDRLVLDLPEPWQVIPHAARVLRSGGIYLSFVPTVPQVQQTVTALQ
                     RAAVFGMVETFETLLRTWSVAGRSVRPDHRMVAHSGFLTVARKVEPGFCMPVQDVQAT
                     NDEDRHEDADGKDQGHASSGG"
     gene            21928..22689
                     /locus_tag="VFH05_04860"
     CDS             21928..22689
                     /locus_tag="VFH05_04860"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6677573.1"
                     /translation="MHRLEGKVAIVTGGNAGIGEAVAKRFADEGAAVVVTGRRQAELE
                     RVISGIRHNKGKALAVAGSVTDEAHVQEVVQRALDSFGRIDILVNNAGIGDFGKRLHE
                     TDDSAWATVIDVNLTGVFRITRAVVPHMLKQGRGAIINISSVASLVGIPGAPAYAASK
                     GGVDALTRAMAIEYAQDGIRCNVVNPGLVDTPMAAPLMANPDMLRPILAHYAIRRPGT
                     SEEVANMVLYLSSDEAGWVTGGTFTIDGGMTVSKG"
     gene            22730..>23241
                     /locus_tag="VFH05_04865"
     CDS             22730..>23241
                     /locus_tag="VFH05_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET6677574.1"
                     /translation="MDINTATQFCGVIGNPIEHSLSPAIHNAAFQKLALNFVYLAWKV
                     DRIGDAVRGLRALGNFRGASVTIPHKVAVLPFLDEVDTTARHIGAVNTIVAEEGQLLG
                     MNTDATGALRALREGNAPLKGASVVIVGSGGAARAIAFALAAEAEVRRLHLLGIEVDE
                     RRNLATDLRD"
BASE COUNT         4968 a         6313 c         6963 g         4997 t
ORIGIN      
        1 ggtccgccgg gcctgggaaa gacgaccatt gctcacatca ttgcacttga aatgggcggg
       61 acgttttgcg ccacctccgg gcttgtatta acgcatgccg gtgatctggc tgctatcttg
      121 acgaacttac aggaacacga cgtcctcttc attgatgaga ttcatcgctt gccggccgcg
      181 gtcgaagaag cgctgtatcc ggcaatggag gatttccagc tcgatctggt tgtcgggcaa
      241 gggccagcgg cgcgaacggt caagttagac ctccctcggt tcactcttgt gggagcgacg
      301 acgaaggcgg ggtctctaac ttcgccgctc cgagaccgct ttgggctcgt tcatcggctg
      361 gaattctatt caccggccga actggaagtc atcgtcatgc gctcggcccg tttgctcggc
      421 atccccattg accaggaggg cgctgcggag atcgcctgcc gcgcacgcgg gacaccccgc
      481 attgtcaatc ggctgatcaa gcgcgtccgc gactatgcgc aagtgaaggc tcagggagag
      541 attacccgag cagtcgctca agaggccctg gcgtggctgg gggtcgatgc cgccggtttc
      601 gatgagatgg atcggcatat cctcctgacg atcatcgata aatttggtgg cggaccggtg
      661 ggggtggagt ctctcgccgc cgctgttcaa gaagacagga gcaccctcga ggacgtccac
      721 gaaccctacc tcatccaggc tggctttctg gaccggaccg gtcgaggccg gcaggtcacg
      781 aaagctgcgc tcgaccactt caagcggccg tcttctctcc tctactgagc cctttttcgt
      841 ttcgcagccg cccgcagtac ggatcacaca agttcctgaa agtgttaggc tctccttgct
      901 ttcctaggag cggttcctgt atcattcgtt gcttactcac gcccgtcctg cctctggacg
      961 ctcgtccaac ttatggggca cgggtcgagg gggtcccatt catgcccggt gacctttccg
     1021 attaccggaa agaaatcgat cgcatcgatg acgagatctt acgtctcctc aacgagcgtt
     1081 ccaaaagtgt ggttcaaatc ggcagactga aaatgttgca agatgccggg gcgaacttgc
     1141 atacgcccgc gcgcgaggcc gccattatcg agcggctcgt cgcccaaaat accggtccgt
     1201 tcccttccga tgccattcgc cccgtctacc gggaaatcat gtcggcatct ctgtctctgg
     1261 aaggtccgca aaaggtggcc taccttggac cgcgggcgac gttcacccat atggcctgca
     1321 cgcaaaagtt tggcgcgtcc gcgcagtatg tgccggtcaa cagcatcaag gaagtgttca
     1381 gcgaagtgga gcggggtcgt gccaattttg gcgtggtccc gatcgagaat acgacagagg
     1441 gtgtggtgaa ccataccctc gacatgttta tcgattccaa cctcttgatt tatggggagg
     1501 ttcttcagga agtctcccat catctgatgt cgaagtccgg ggctgcggag gacataaaga
     1561 aaatctgctc ccatccccac gccatcgccc aatgccgaaa ctggctcgag acgaatatgc
     1621 cccacgttcc cgtttcggag gtcgcgagta cggcccgggc agccgagatg tgcctggacg
     1681 attcctcgat cgcagccatt gcttccgagt tggcttcgca gctctatgga ttaaaggtca
     1741 tcaagtcccg catcgaggac aatctcaaca actttacgcg gttcctcgtg ttgtcgcaaa
     1801 aaccaccgca acgcacaggc aaagacaaga cgtcgctcat gctctccatt aaagacaagg
     1861 tgggtgcgct ctatgacttg ttgcgtccct ttgcgtctca tgggctgagc atgaccaaga
     1921 tcgagtcgcg cccctcacgg cgcaaggcct gggaatacat tttcttcgtc gacatcgagg
     1981 gccatattga agaggagcgc gtcaagaagg cggtcgagga aatcaccgga cgctgtctct
     2041 tcatgaaagt cctcggctct tatccagccc atagctgatt ctatgacgct tcacgtccac
     2101 cctgatatcg ccgcattaag tccgtacgtg ccgggtaagc ccatcgaaga actccaacgt
     2161 gaattgggcc tgagccgagt cattaagctg gcctcaaacg aaaatccgtt gggaccgtcg
     2221 ccgaaagcgt tggcggtgat caacgagtgt gcggcgacat tgcatcggta tccggacgga
     2281 ggggcgttca aattgcgcca ggcgctggct catcgatgga aagtgaccga cgagcagatc
     2341 attctgggta acggctcaga tgaaattctc gggctcctcg ctcgaacgtt tctgtcgccc
     2401 ggtgacgaag cggtcatggc cgaccacacc ttcgtcattt accacatgga agtcatggcc
     2461 gcgcatggca aggcggtcac cgtcccgctg aaagactggc gtcacgatgt tgctgcaatg
     2521 gccagtgcga ttacggctcg cacgcgcctc gtgttcctgt gcaatcccaa caatcccacg
     2581 ggaacgatgg tgtcggccga agaagttgca tggctgctcg ggcgcgtccc tgaccacgtc
     2641 gtggtggtct tcgatgaggc ctattttgag tatgtgcgca gttccgagtt ccccgattcc
     2701 atggcctgga tcaaggaagg acgccatgtc atcgtgctgc ggacgttctc caagatctac
     2761 ggcctcgccg gattgcgcat cggctatgga gtcacgacgg gcgagattgc gaatcttttg
     2821 aaccgtgtga ggccgccctt caatgccaac agcattgctc agcgcgcagc gcttgccgcg
     2881 ctggatgatg acgagcatgt cgcgaggagc cgggcagtga atcaggccgg catggaacag
     2941 atcgcgactg gattgcgggc gctcggatta gcgccgatca atagtgaagc caatttcgtc
     3001 tatgttgatg tcggtcgcga cggccgttac gtattcgatg cgatgttgcg tcacggcgtg
     3061 atcgtccgtc atatcgaggg gcgtatgata cgggtgacga tcggacaggc agaagaaaac
     3121 cgggtgttct tggctgcact tgcacaggtc ttacagcagg gtgaacaagg gtgaggttgt
     3181 tatgatcatc gtgttgaagc cggaggcatc ggaacgcgaa gtcgatcata tcgtcgatcg
     3241 gctgcgtgaa ctggggttaa aatcgcagct ctcgaccgga caggaacgaa ccatcatcgg
     3301 ggtcatcggc gatgatcgca tacttcaaaa ccagccgctc acagccttgc ccggcgttga
     3361 aagcgtgctg ccgatcctgg ccccctggaa actggtcagc cgagaattta agaaagaagg
     3421 cacgatcatt gatgtcggag gggtcaagat cgggggcaac aagctcgcaa tcatggcggg
     3481 cccgtgtgcc gtcgaacgcc tcgaactgac cgtcggcatt gcgcatgagg ttaaggcggc
     3541 gggagccagc gtgctacgtg gcggggcgta caagccgcgc acctctccct actcgtttca
     3601 aggactgggt cgtgaaggat tggactatct ggctgaggcg agaaaacaaa cagggttgcc
     3661 agtggtcagc gaaattctcg atacccgtga catcgagttg tttctcgaaa aagctgacat
     3721 catccagatc ggtgcgcgga acatgcaaaa ctttgaattg ttaaaagaag tgggcgccta
     3781 cgacaagcct gtgctcttaa agcgtgggct gtcggctacc atcaaggaat ttctgctgtc
     3841 ggcggagtac atcatgtcgc gcggaaaccg gaatgtgatg ctctgtgagc ggggtatacg
     3901 caccttcgag acgcaatacc gcaacaccct cgatcttgcc gctattccca ccttgaagga
     3961 gctgtcccat ctgccggtca tcgtcgatcc cagccatgcc accggcaaat ggaatctggt
     4021 ggctccaatg tccaaggctg ccgtagctgc cggtgccgac gggattctca ttgaagtcca
     4081 ttcgaatccc gaatgcgcat tatgcgatgg cgaagagtcg atcaagccgc tcaagttcaa
     4141 ggaattgatg catgatatgc ggaagatcgc gatcgcggtg ggaagagaag tgtaatggcg
     4201 cggctttttc gccaggccgc cattatcggt gtcggcctga tcggtggttc tctcggcatg
     4261 atccttcgcc ggaaaggatt ggcgtccagc gtcgtgggcg tcggacggcg catcgagaac
     4321 ctcaagctgg cggtcaaatt aggggcgatc gaccattacg tcgtcgatgc caaggagggc
     4381 gtgaaagatg ccgatgtcgt cgtcctggcg acgccggttg atacctatca acgccacttg
     4441 gaggaatggg cctcgtgttt gaagtctggt gcgattgtgt cggacgtggg aagtgtgaaa
     4501 ggcgagttag tcgagcagtg cgaacggctc atgccggacg gcgtccattt tgtcggcgcg
     4561 catcccatcg ccggcaagga gaaaacgggc gtcgccgcgg ggtcggagga ccttttttcg
     4621 ggaatgcggt gcatcgtgac gcccaccaaa cagacgaatc cgcaggcact cgaacgggtg
     4681 aggacgctat ggcaggaagc ggggtcgacg gtgctcacga tggatccgca tctccacgac
     4741 aaaattcttg gagcggtgag tcatctgccg cacgtcgtcg cctttgcatt aatgggcgcc
     4801 ttgatcgata tccgtacgga ggttccgtcg ctcgatctgg ccggccattc cggcggcggc
     4861 ttgcgggata cgacccgaat cgccgccagc tctcccgaga tgtggcgcga cattttcctg
     4921 tggaaccggg acaatgtggt cgcctttatc gaggcgtatg aacgctcgtt gggagagttg
     4981 aaacaattga ttcgcgcggg cgatgcggcg gggatcgaaa aggcgttgga acgggcgaag
     5041 caggaacgcg agaaattccc ggtccgcacc ggatagttcg gctggaggag aacgggccaa
     5101 cttgaagaca ttgacgatca caccgggccg gccgctgcgg ggaaccattg ccgttcccgg
     5161 cgataaatcc attacgcatc gggcgattat tcttacggct ctcgccgaag gcatgggacg
     5221 cattaaacgc tattgccgtg gcgaagactg cctcaatacc atgcgtgcat tgcaggcgtt
     5281 gggtgtgcgg atcgatgaag ccgacgatga gctctgtgtg catggaaagg gtctgtgggg
     5341 actgaatgaa ccggcagtgg tcctcgattg cggaaactcg ggtaccggaa tccgtctgct
     5401 taccgggctt ctggcgggtc aggatttttt cagtgttctc acgggagatg cgtccattcg
     5461 acgtcgtccg atgggacgaa tcgtcaaacc tctacgcgag atgggcgcca cgatcgccgg
     5521 gcgcaaaggt ggagaactgg cgcccttggc cgtgacaggg acaacgcttc gtgcgcttac
     5581 ctacacctcc cctgtcgcca gtgcccaaat caaatcctcg ctcttgttcg cggcactctt
     5641 cgccaaaggc acgagccgtt tctctgagcc caggctctcg cgtgatcata ctgagcggct
     5701 gtttcaattc tatggcttgc cgctgactcg cgacggcctg actttgacaa tggaggggcg
     5761 accttctgtc ggttggactg ctgcagctga attgaccgta ccgggggatt tctcggccgc
     5821 ggcctttttc attgtgggcg ggaccatcgt gccggattcc gacgtgacga ttaccggcgt
     5881 tggtatcaat cctacgagga ctggactcct cgagattctc cagcgcatgg gcggcgacat
     5941 tcaggtggaa aactatcgag acgaagctgg cgaaccggtc gcggacatcc gtgtgcggtc
     6001 tgctccgctc catggggtaa cgattggtcc ggagtggatt cctcagtcta ttgatgaatt
     6061 tccgattctc tgcgtagcgg cagccgtcgc gtcgggtcaa accatcgtga ccggagccca
     6121 ggaattgcgc gtcaaagaga gcgaccggat cgcgaccatg ggcgccgaac tccgtgccat
     6181 gggtgcacag atgaatgaga aggctgacgg gatggtcatt gaaggcctgg ggcacgctcg
     6241 cggcaacgga cgtctgagag gcaccgacgg gaagagtcat ggggatcatc gggtggcgat
     6301 gtcaattgcc attgcagggt tgacggcgct ggacgaaacg cgtgtgcacg acgcggactg
     6361 tatcgagacg tcgtttcctg atttcgatca ggtccttacg caactgactt cgtagaagaa
     6421 aggtgtctga gttccatgat cgtggcgatc gacggcccag ctggtgtggg gaaaagcacg
     6481 gtagcaaagt tgttggcttc gaggttggga tttttgtatc tcgacacggg ggcgctctac
     6541 cggtccgtgg gtctggcggt tcttcatcat ggtatccagc cccacgacga gacggcagtg
     6601 gctgggcttc tgccgaagct gtctctcgag attgaggtcg cgaaccacac cgtgcacgtc
     6661 tcattaaatg ggaaagatgt cacgggagaa ttgcgaacgc ccgcagtttc tgcagccgct
     6721 tcgatcgtct cagccattcc cgacgtcagg gcctggctgt tgccgatcca acgacagatc
     6781 ggacgaaaag gttccgtcgt ggcagaagga cgtgatgtcg gtacgaaagt gttcccggcc
     6841 gctccggtga aattttttct tgaagcagat ccgaccgttc gcgccgaccg ccggcatcgc
     6901 gaactggtgg ccgccgggca cagccgggcc atcgagcaga cgagtgccga gttagccggg
     6961 cgggacaacc gcgaccggtc gcgatccgcg gcgccgctgg tggcggctga cgatgcccat
     7021 catattgata cgtcccgttt gtcggccgaa gaagtcgtcg aacggatgtt ggcggtggtg
     7081 acggccaaat tgtgagttcg gttgcatatg ggatcttatg ggttctggcg cgggcgatcg
     7141 cgtggctctg tttccggttt cgcgtcgagg gcgcggtgcc gaagcaaggc ggtgtgcttg
     7201 ttgctgccaa ccatgccagc tatttcgaca tcccgttgct gggttgcggc atgttcaggc
     7261 gcgcgtggta tctgggtcga agtgatttgt ttatccccgg cttgaaggtg atcttgcgat
     7321 ggcttggctg gatcccgctc aagctgggaa ggctggatcg taaggcaatc gatcaggcga
     7381 ttgccctgat taaggcgggg aaggtcgtgg tgatttttcc agagggaggc cggagtctcg
     7441 atggccgtct gcgacaggcc aaggctggtc tcggtatgat cgtcgctcag accgggtgtc
     7501 ctgtggtgcc ggcgtatctc aagggcacct ttgacgtgtt gcccgccggt gccaaacggc
     7561 cacggttcca ccccgtgtct gtgtcattcg gtccaccgct gtcattctcg actaccggga
     7621 acggtgacgg gaagcagttt tatcaaaatg tgagtcgagt cgtgatggag cggattgccg
     7681 cactcggcgg ggtggctccg ccgccgcatc atgcaagtgc cggcgctcgc aacgctgagt
     7741 gagcgccggt gttactgatc atcagcaccc gacagtcgtc ggaagagtag gatcgtcccc
     7801 atgagcacgg tttcgaaaac gaacgagccg cagttggatc gtgaagcact ggccgctctc
     7861 tacgaggaaa cgttcaaaaa cctcgaagaa ggcaccatta cggaaggtcg ggttgtcgcc
     7921 ttggccaagg acaaggttgt ggtcgatatc ggatacaagt cggaagggat gatccctagc
     7981 gaccagtttt cccatgacga tctgcagcag ctaaagatcg gcgaccggat tcaagtgtat
     8041 atcgaggaat gcgaggacgc agacggcaat ctcattcttt ccaaagaaaa agccgacaag
     8101 atgaagattt gggaagaatt ggaaaagctc tataaggatg agaagagcat cgacggtaaa
     8161 gtggtttctc gcatcaaggg cggcatgatg gtggatattg gcgtgaaggc gttcctgccg
     8221 gggtcgcaga tcgatctcca tccggtgcgc gatctggatg gattggtcgg ccgaacgttc
     8281 ccgatgaaga tcatcaaaat caatcaccgc cgtgggaacg tggtcgtgtc tcgccgcgtg
     8341 ctgctcgaag aaacccgaga caagaaacgc cagacgacgc tcgctacctt aaaggaaggg
     8401 cagttgattc aggggatggt caagaacatc actgattatg gagccttcat cgacctgggc
     8461 gggatcgatg gcttgctcca catcacggac atgtcctggg gccgtgtcgg acatccttcg
     8521 gaaatgttca acgtgggcga tcgtgtcgaa gtgagtgtgc tcaagtacga tcgtgaaacc
     8581 ggcaggattt cgctgggact caagcaaaaa tctgccgatc cgtggacaaa tgtggcgggg
     8641 aagtatccga ttggcacgcg cgtcaaaggt cgtgtcgtca gcctgaccga ttacggggcg
     8701 ttcgttgagc ttgaacctgg agtggaaggc ctggtccatg tttcggagat gtcgtggacc
     8761 cacgaagtgc gccatccgtc acgtgtggtc gccatcggcg atcaggtcga agccgccgtg
     8821 ctcaatgtgg atcccgcgag ccgaaaaatt tcgctcggca tgaagcagac ggcgccgaac
     8881 ccctgggaca tggtcgaagg aaagtatccc ctcggcaccc gcatcgaagg aaaggtgaag
     8941 agcctgacgg atttcggcgc cttcatcggg ctcgacgaag ggatcgacgg attgatccac
     9001 atttccgaca tgtcttggac caagcacatc aagcatccat cggagatttt caagaaaggc
     9061 cagaaagtgg aggccgtcgt cctgcggatt gacaaggaaa aggaacggct gtcgctcggc
     9121 tacaagcagc tctcgcgtga tccatgggac gacgagattc ccaatcggta ccgcgtggga
     9181 gatccggccg tcgggaaagt gagcaaagtg gcggatttcg gaattttcgt cgagttagac
     9241 ggcggtgtag aaggattgat ccatgtcagc gagtcgggat tggatcaaag tgcgaagctc
     9301 gaagagaagt tcaagttaca ggatgaagtg acggcgaaga tcatcaaggt cgaccgtgaa
     9361 gagcggaaga ttgcgctcag cctccgcgat catcagttgg actccgaccg tcggcatgta
     9421 gatgagtacc acgcgactca aggtgctcct gaccagagtc tgggacgggc agccaaacaa
     9481 agccgcaaac gcggagccga cgacgacaaa tagatggtga cctctcggcg ccggagcgtg
     9541 ctgctccggc gccggctaat gcgtggagtc ctatggagca ggagacaaca ggaaaaccgc
     9601 caaagcgatc gtggcttcgc cggattcttc tcgtggcagg catcggtatc gctcttctgc
     9661 ttctcctcaa tgtattgttt cctgatttgg atctgtcgac ggaggaccgt gtcgcgttga
     9721 ttcgtgtgga aggggtcatc ctggatgctc aatccacggt cacggaattg aaacggttcg
     9781 gagaaaaccc atccattaaa gccattgtct tgcggatcga cagtccgggc ggcggggtcg
     9841 ttccgtctca ggagatctat gacgccgtgc agcgggtgcg caacaagcac aataaggcgg
     9901 tgatcgcttc gatgggcacc gtggcagctt cgggcggcta ttatatcgcc gcggcaacgg
     9961 atcgcatcat cgcgaatccc ggcacgttga ccgggagcat cggcgtgatc atggaaacgg
    10021 cgaatgtcga agggttgctc aaaaagatcg gcgtcgaagg tattgtcgtg aagagcggaa
    10081 agttcaaaga cgtgggctcg ccactcagga aaatgaccga ggaagagcag aaactgcttc
    10141 aatcggtgat ggacgacgtc caccaacaat tcatcgatgc agtggcagaa gggcgcgcgt
    10201 tagaaccaac ggcggttcag gcattggccg atggtcgaat ctttaccggc cggcaagcca
    10261 aggaagcaaa gcttgtcgat gagttaggca atctggatga cgcgattcag ctggcggccg
    10321 atttggccgg gattgaaggt gagcccaagg tcatcgagcc acgccggcgg ttctccatcc
    10381 gggagtttct cgagtcgcgg ttctcggcgc tgttcccgaa gttggacttc tattcagggg
    10441 tcagcctcaa atacctcatg gcgttttaat cggtcggagc ggctggcagc tgctcgtcca
    10501 agcgaaaggg gaatccgtat gaccaaggcg cagattattg agcgggtctc tgagcaagtc
    10561 accacgctga caaaacgcca agcagagatc gttgtgaaca cgatcttcga ttgcgtcaga
    10621 gactcactga agaatggcga caaaacggaa atccgagggt ttgggagctt tcgcctccga
    10681 tcacggcgga tgaaggaagg acgcaatccg aagaccggcg ccaccgtggc agtgcctgcc
    10741 aagaaagttc ccttcttcaa ggcggggaag gagctcaagg aattgctcaa ccagtaggat
    10801 atataaagga agatccaatg gctgcatcag attccaacga tacggctacc gcagaagtcc
    10861 gatggaccga aggcgcattg aagcgcatgg agcgcgctcc gatatttttg cgcgggatgg
    10921 tccgccgtct ggcggaaaaa aaggcacggg aactcggcta tgctgagatt accgaagcca
    10981 cgctggaaca gttcaagagc cagatgatgg gcgggatggg cggtgcttcg ggcatggccc
    11041 aggccgcgga agacatggcc cagggccggc tgccatggac ggcggcggcg aaggagcggt
    11101 tgaataccgt accggagttc atgcgcggca tgatcaggca aatcgccgaa gaggttgcca
    11161 gggaaggcgg ccacatggaa gtcaacgtcg acctctttga gcgggtcgag gccatggggg
    11221 aggtgcgcga ggcggctgcc cccccgctcg cctggacgga aggagccctt gcgcgtctgc
    11281 aggacaagct gaagcagtcg cctcccatcg cagtggaatt cgtcacggac atgctcaagc
    11341 gtgatactga agatctcgct cgcgagaagg gtttgaccca gatcgatgag caggtgctca
    11401 tccaattatg ggacgctccg caagaacgag tcgcctggac ggacgaagcc tggaaacggc
    11461 tccagacctc tcctgacttt gtgcgaagcg ggattcgcaa ggccgccgaa cgacgcgcgc
    11521 gaaaactcgg tttgaaggaa attgactcgg aacacctgac ggggtttcga aatcaagcga
    11581 tgatgaaagc cgtgaaacga attcgttcgt tcgggtacaa cgagctgacc ttcgaggcct
    11641 ttgacaccgc gcttgagaag accaaacggt tacaaggcaa tgaacaagcc gaaaaacgtc
    11701 ttcaggaaat tcgcagccac tttgccgatc ctgacacgaa gaaacccgag gggggaacgc
    11761 tgggggctga attgatgggc cgcttccgac gctacctcaa gggggaagga agtctgtaac
    11821 agtggcccag ggggttcctc ttccggggcc atctctttat ccgggcttct ccaaaagcat
    11881 tgtcgtaacc gcctaattct cgttcctagc ccggtcaatg cgagaccggc cctggccagc
    11941 cggcgccaac caagtaggat agtccgagtt ggcgggttgg tctcagtgcg ccgcgatctg
    12001 ctgtggccaa aatttgtgtc gaatctgcgg gagcggctcg ttgtcgaggt tgggaatgtc
    12061 gtacagacaa cggtcgatca gtgccacttc ctcttcggtc agttccaagg cgaccttttt
    12121 cttccagaac tgatcgaggg tgaaatagtc gccggactgc ggcttctccg ccaacagcgc
    12181 tttcatcttc tcgaacccgg cagtatccac gccgggaatg cgtcccttat tccggtcgtg
    12241 acaccaatgc aggacttctg caattccata catcgtcgta tcgatcttca cggtcttgcc
    12301 catggattcc tccttgcaaa aggactgtag tctaacccag attcaaacgg agtttcaaag
    12361 cccatcttac cggtgcaatc gttccggctc tgggcctgtt cgttgccagg agaaaggcgc
    12421 cgccggtata ctagcgagcc ttcaacacgg aatcacgcct gggagcagtg gacggacggg
    12481 aggctgtctt gcgggaaatg cgtgggttca caacgacatg gagctgcagg tgtctcatct
    12541 cgggcctgct cgtcgtgatg agcggtgcct gctcgaagca ggatccgtac gtcccgcccg
    12601 atctcttcta ttacttcgcg agctataaag tcggtaaaaa ccccacgacg atcaccccct
    12661 ttgacatcaa ccaggacgga gtgaccgatc tcatgacgac caatgtgagc agcaacacgc
    12721 tgtcacttct gatcggtaac ggagacggaa ccttccacga tcacgtgcag cttcacgtat
    12781 gccaggaacc cagatcgtta gccaccgggg cattcaatgc cgactcttat ccagacatcg
    12841 cgttggcatg cgccggcggg gatgaggtcg ctgtcttgtt tgggcgagca gacggcaagt
    12901 ttcaggaagg cccccaatat cctgtccatc ggacaccggt ggcgattgcg agtgatgatt
    12961 tgaacggcga cggcatggcc gatcttgtgg tcgcgttgcg caatgacaaa atcaaggtgt
    13021 ttttggggca gggcaacggt gagtttcgac acggggcgca gtatgagtac ggcgatacac
    13081 cgacctcggt cgctttgtcc gatctgaacg gcgacgggaa gaccgacctg gtcgtgacaa
    13141 acggaggacc gatgtccaat gccgtgtcca tttggcttgg aaatggagac ggcagctttc
    13201 gatctccgac tgattatcgc tccggtcgac gccccttggg cgtgagcgtt gccgatttta
    13261 acaacgaccg catccgcgat cttcttgtga tcaatggtga acacgacagt ttttccatat
    13321 ttctgggaaa tggcaatgcc actttccagg cggcacatga ttctggcgca aatgccggcc
    13381 caaacttcgg gctggcccgg gacttcaacg gggatcatcg gaccgatgtc gcgattgtca
    13441 acttgcagtc gagcgatctc tcaatcctgt tcggccgtgg agatggcaca ttcgaatatc
    13501 caccgaggaa ctaccggaca aaggcaggtc cgttcgctct ggcgctgtac cgagtaacga
    13561 gcactgaagt cgaagagcct gggctcgtga ccgccgacaa tggcgacggc tcagtctcga
    13621 ttttcctcca tcgcggtttg aaagcggggg cgaggccaaa gaccgccgcc tcctcatcgc
    13681 agtcgactca atagggtatc acgacggagc ggcgaaacag cgcagtcagc ttgacagtcc
    13741 tggacccctc ggctacagtg gccgacaggc gccgtttgat gtagctggct gcctcgtacg
    13801 gggacagcgg tgagatcctt ttcgtggtgg ttcggaatgg gcgcactcct gaccctgtcc
    13861 gttctgatgg ttcaaggagg cgtgcgcgac ttgtttgacg gttcgcatcc cagcggcggt
    13921 gcgagcccct acgtgtcttt cgccaccaca atttttggcg gtggactgtt ggccggttgt
    13981 ttggcactgg tgatgaagcg cattcgatag accgggtacg agcgagagcc atggattgtc
    14041 tcaagtgcaa agggttcatg atggtcgagc ggcactacac gctcatgaat cggcgtctct
    14101 atgcccgctg tctgaattgc gggttttgga tcgacctcgc cgatctcctt cgttttttcc
    14161 ataaagtcat cgacagtgga ttccggggca gcaaggtccg ggaatcgttc actctttgag
    14221 gaagactgtg caggccacgg tgttttcttt gacccagcga acgtcgacgt tcttcctcgc
    14281 ggctctctgc atcgtgttgt tggcgccttc gacagttctc gccaaacgtg ggaatggaaa
    14341 gaccaggcat gtcactgcca ttagcgaaga ccatcaccac ttgccctggc gtcgggtacc
    14401 ggctcacagc attcttctca aagagttgaa cagtggccgc gtactgtatg agcacgactc
    14461 ggcgaagcgt ctatctcccg ccagtctgac gaaaatcatg tccgctctgg ttattttgga
    14521 gcatggaagg cttgacgatc tggtgactgt gagtccagcg gccgctcgag cgcacaaaac
    14581 gcatctccgg ctgaaagcag gccaagtgtt caaacttgac gacttgttga aggccatgct
    14641 gatcgtatca gcgaatgacg cctgcttagc tgcggtggag catgtcgggg gggacgaaac
    14701 gcaatttgtc ggcctgatga acgacaaggc cgcggccctg ggtttgcata atacacactt
    14761 cagcaacgga tgcgggtttg acggtccaga gcactattcg accgctgagg atctcgcgac
    14821 gttgagcatc atcgctctga tgcagccgat tttccgagac ctggtgaagg aagagcgagc
    14881 catcatcatg ccggtcaacg gcaaccatcc ttatattctg cacactacga accggctgtt
    14941 gggaagaatt ccgggtgtgg aaggaatcaa gactgggttt acctccaagg cagggcgttg
    15001 tctcattgcg aaggtgtcac aaaacggcag tgatctgttg ttggtgattt tgaactccag
    15061 ccggcggtgg aatacggcga ccagcctcat tacctacgga ctgcgggcgt caggcccttc
    15121 cctctagcgg gttcgcccct cgaatagata tcgtcagatg aagaggatct caacgaagcg
    15181 gcgttggtgt cccagcacgg caagctcagt gaacgggtga aaattcgatg ttgacgtctt
    15241 tgccgagata attgatcacg aatccctgct tgtcgtacgc caacacggcc cccatgacgt
    15301 tttcatcccc gtaatcttcc tggcctaata gttttcgaat atcttcgggg ctctcgctgt
    15361 tcagcacgtt gcgaaagagg ccgcgtgtct tataggcaaa acgattgttg atgaagatca
    15421 gatctacttt gccatcttgt acgcgcaccc ctgccatagg accggtgggt ttcctttgat
    15481 cgaccagaaa gatgaataac gcttcttgtt cgaccttgat gccaggtatt ttttcattga
    15541 ccaggacgtc catggccttg gattcggtat caccgatttt gacgccgaaa agagaaatat
    15601 ccggactctt gatcgccttc ggatccatga catcgttttt gctcagctcg tagggttccg
    15661 caagcgccga tgctacgacc agaccgatta tgcctatgac gaggcatgat cggacaccca
    15721 ttcgtatcag cgcttccatc ggctcctcct ttttccctga ccccaaactc cggtgcgaag
    15781 atttgatctc aaacagcgct aaattcctca tttcattcta tcggaggcca cagccggttg
    15841 caatgaatcc tgcgccgaag tccggccggg cgaagccgct aagaactgcg gcctgcgatc
    15901 aggagcaacg ttccgagctc acggagacgc tggaaatacg cccggcattc ctgatcaagc
    15961 tcctgttcca tggcttcaag atcggccaga cagtctccga cgatggtacg acgttgctcg
    16021 tccaatccgt cttcgctgtc tagaaaatag acgacgcagc cgctgatcac cgtatccaac
    16081 gtcatcagcg gtccttcaac ttggctggcc acccgaggcc attcctcctg tttatgggct
    16141 tcccatagcc ttccgaccgt gtctcggtcc actgaaccgc tcctcaggtg ggtgtccgtt
    16201 attgatccac tcacatgcaa ttgctacgga taaaggccgc gatgcaaatg agccttggca
    16261 accttatcga ttccgatcat gagcgcggct atccgcatgg tcgctcgctt ggtcgtggcg
    16321 aactgcaggg tgcgttggaa ggcggaagtc atcagatcgt tgagacgttc ttgaatatct
    16381 gtcgctttcc aaaaaaaccg ctgggcatcc tgcacccatt cgaaatacga aacgatgaca
    16441 ccccctgagt ttgccagaat atcggggatg acgaaaatcc ctttgtcgtg caaaatacgg
    16501 tcagctccga tagtcgtcgg cccattggca ccttctgcca atacgcgaca gttcagtttg
    16561 ggggcattgc ggtcggtgat ctgctctgat aaagcggcgg gaacgagcac cgtgcaattg
    16621 agttgaagaa gctcatcgtt cgtgatgcga tcccctaact tggtatccgc cagcgtctgc
    16681 ccggtgcctt tgtattttgc gagcagggtg ggaatatcga gcccgccggc gttgtagata
    16741 ccaccctcca catcgctgac cgccaccacc tttgcgccgg attcgtacat gatgcgagcg
    16801 gtgtgcgatc cgacgttgcc aaaaccttga atggcgaccg tggtgtgacc catctcgata
    16861 cggagatgtt tgagcgcttc ctgaatgacg caaactactc cacggccggt ggcttcttct
    16921 ctgccgagac ttcctcctat ggaaaggggc ttgcccgtca ccacccccgg gacagcgtaa
    16981 ccgacttgct ggctgtacgt gtccatgatc catgccatga cctgggcatc cgttcccaca
    17041 tccggagctg ggacgtcctt ttccggcccg atgagaggga aaatctccgc cgcataccgg
    17101 cgggtcaggc gttggagctc gcctcgggac agttgtttag gtgctacggc tacgccgccc
    17161 ttggcgccgc catatggcag atcggccagg gcacatttcc aggtcatcca catggcgagt
    17221 gcggctacct cccccagatt gacgtcaggg tgatagcgaa tgccgccttt tgacggcccc
    17281 cgagacgaat catgttggac acggtatccg gtatagacct ccacacgatt gtcatccatc
    17341 cggataggca cgctgacgac cagcgatcgc tgtgggaatt tcagtcgttc acgcagattc
    17401 gcatccaggc ccatcaattc tgcggcttca tcgaactgag cgactgccag tctgaatgtc
    17461 ggcgtgtcga gttcatgcag ggaagacatc agcggaagtc ctctcccgag ggcgaaatca
    17521 tgggctcctc tccaatcgag ggcggcatgg tcgttcccgg ataggggcac ctccactcga
    17581 gctgaaacac ggatgagaag ctttctgcca cgtgccggcc gactaactca agcgacacag
    17641 gcgctcccat gatctccgcc atcgaggtca tgcggcaacc gtcgagacca cagggcacaa
    17701 ttcgggaaaa cggagagaga tcaagatcca cattcagtgc gaacccgtgc agcgtaactc
    17761 ccaggtcaac acgtgcgccg atcgaggcga ttttggcttc accacaggac gaccctgtcc
    17821 aaacacctgg cgtcctggtg agacggtagc catttacatt caaacgagcg agagttctca
    17881 ggaggacctc ttcaagcatg cgcacataag tttttggtcc gggcgcataa cgagagagcg
    17941 ccagaatcgg atagacgaat agctgtcctg gtccgtgata ggtgacggac ccgccgcgag
    18001 tgacggattc gattggcacg tcgtcatggt cggaagaaag atggagtctc acatgcccgg
    18061 gtttcgtgcg acggccgaca gtataaactg gcaaatgctg aaggagtacg agcgtgtcga
    18121 ctcgccttcc cgctacacgg tccgagtgaa gtcgttgctg taggcgccag gcgatcccat
    18181 actgaacggg cttgctgatc gtaatcagtt ctccttgggg caatggcgtc ttgatagagg
    18241 gatcgagaat ctgtgcccct gcaggaaaat caatggccgt cactgtgccg ccttcaacgc
    18301 agccggtttg gggagctgga ttgtggccgg accgcttctg ccgtgcaacg tgagctggac
    18361 ggtaggttga tctcctgaac gaagcagcgc atgccaaaag tctcccatgt tgaacacctg
    18421 atgctgatcg accgccgtga tgatgtcgcc gttttgcaaa ccgccgactc ccgcaggttt
    18481 tgctgcatcg acctgtaagg caagcacccc gcgatccccg tccagaccaa agctcgccat
    18541 gacgcttggg gtaatggtca ccggtgtaaa ccccaaatcc ggtcggtaga ttgacccgcg
    18601 aaccatcatg gattgaatgt gagggtaaat cgcctccatt gataaggcat aactgatggc
    18661 ttgcgccgat ggcgcaatga tgacgttgat gcccaccact cggccggaca agtccacaag
    18721 ggggccgccg ctattgcccg ggttgatcgc cgcatcggtt tgaatcaggt cgtagagcgt
    18781 ttcgccatcg ggagtcaaga ccgatcggtc caacgcactg acgacgccta cggtcacggt
    18841 agagccaccc ttcagtgcca aggggttgcc gatggcgacc acggactccc cgatctccag
    18901 atcgggtctc ggattcaaca ccgccggcac gagatcctgt gcattgattt tgacgagagc
    18961 aaggtcgagc agaaaatccc gagcgacgac gcgacctggt gtcagacggc cggtcgaaag
    19021 tcctacgacc atgcttttga cgccggtgac aagatgatta ttggtcagaa tatagccccc
    19081 ttcgtcgatg atgacacccg accccgagcc agagcccgac tggacctgtg acgggctggc
    19141 tggcacgccg cgcgtcagga tggtgaccac agaggaccgg actttggcca cgacggctgg
    19201 gacggagagg gttttgtcgg tcgagaccac ggcgctcgaa cccgggttgg cccaggcgcc
    19261 ggaagagccg aaaagcagac ttgcgacagg aatcaagatg agaggaaggg tagccataga
    19321 ccccacacgt gaactgcgct cattgtctca caactatcga tcccggaaaa ggcgcaacaa
    19381 aaaaggggtg aggagctgcc tcctcacccc tttcgcgtgc cggtcttgcc attattgtat
    19441 ggcggcaaac atttccttta tcgccggtgt cttgagcttc ttgagcgctt ttgcctcgat
    19501 ttgtctgatc cgctccctcg tcacggagag gttctggccg acctgttcga gcgtgctggg
    19561 ttggtcatag ccgatgccaa agcgtagacg aatcaccgtc tgctcacgcg gggtcaaatt
    19621 ctccagaata cggtcgagtt gatgagtcat ttcggtgcga tgcacgtgcg cgtcaggaga
    19681 cacggcctgg aggtcaggca gtagctcccc gaactccgtc cccccgtcac ccacggggtg
    19741 ttccaacgcg acgggttcct ggaaagcttg caccgtctca tgcagccgtt cgggccgcat
    19801 ccgcaacaca ttcgcgatct cctccaattt ggccggccga ccaagttgtt gccccagccg
    19861 cctggtcacg cggagaatcc ggtgcgaggc ttctgtctgg tgaacgggaa ttcgaatcgt
    19921 acggctttgg tctgccaggg ctcgtgtgat tccctgccga atccaccagg tggcatacgt
    19981 gctgaacttg aatcctttcc ggtattgata gcgttcagcc gctttcatca ggccgatatt
    20041 cccttcctgg acgagatcca gcagcgtcaa gccgcggcgc gtatagtgct tcgcgacatc
    20101 caccaccaaa cgaaggttgc aacggaccaa ttcatccttg gctttctcga gcacttgccg
    20161 tgcctgccga actcgtgcga gatcgctctt cagcgcttta atcacagtcg ccggcacctt
    20221 gaaaccgtct tctcccttta tcagatccag caaacttcgt tcgactttat cgagagcggt
    20281 agccgataag ccgctcagcc gcctgatggt ctggagcgtt tgctgaactt ccgtaatggt
    20341 ctgtgttcgt ttcacgctcg ccaagagacg atgcacgtgt aggagcgtcg cccgaatcat
    20401 cgtcgagcct tgatcgatcc gttttgctaa tgccgtttct tcctctcgag tcagcagtgc
    20461 acgttcgcca aaggactgga aatagagcga ttcaagcaga aacgggctgc tcgattcgat
    20521 ctgacgcttt atgggttttg gcgcttcttg tacggattcc acttccgtag aattgcgacc
    20581 gatcatatcc agcaaatcgc tgtcctcgga gtgatccgta tctgcgtctt ttagctccgg
    20641 cgatttcagc tctggaccgt cggcagagaa caggtcgttt ttcatgtaat cttcctcctc
    20701 ctaactaccg tatgaaggtc gtcaatgatc acatacaaat aagtctcttt aggtgagagg
    20761 caatcccagc acaaaagatt cacacgcttg tccctgcgaa tattctacgc agcaagtccc
    20821 atgctggatg gatgatgaga actccgcggc gggatcgcga tgaaaataca tcactattag
    20881 aacggcttaa tgaggaatca tggagagggc tgaggcaatc ctgctgcgaa tgtgtaggct
    20941 ttgagatagc gtttgggtcg atttgtcaca tcccgacggg atcagggata accctggtat
    21001 cggagcacag gtgctgttga aaagccggtg tggcgcttct agaatggcgc gtcatgtcct
    21061 ccacaactct gcaaaacggg gatcggatac acctggtcga caggaaaggc cgccagtatg
    21121 cgttgaccct caaggccggc gatatctatc aaataagtgg ccacaaaatc gcccatgacg
    21181 atctgatcaa taaacttgac ggctcagtcg ttcacctttc cggtggaaag agcatgttgg
    21241 ctatgcgccc tacgtttgga gagtatgttc tgaagatgcc ccggggggct caggtactgt
    21301 atcccaagga cctcgctctc atgacgatgt gggccgacgt ctatccaggc gcccgggtct
    21361 tcgaagcagg cactgggtct ggcgcgttga ccatggcgct cttacgagca ataggccctc
    21421 gaggcgtcgt cgtcacgtat gaggcacgtg aagattttgc gaggaccgcc aggagcaata
    21481 tagaacggta tgtgggtccg gtgccgaacc tcattgcact tcgcaagaat gcttatgaag
    21541 gaatcgacct tctcgaggat ggcgtgccat ttgatcggct cgtgctggat cttcctgagc
    21601 cgtggcaagt gattccccat gccgcgcgtg tcttgcgatc gggaggaatc tatctgagct
    21661 ttgtcccaac cgtgccccag gtccagcaga cggtcacggc ccttcaacgc gcggccgtct
    21721 tcggcatggt cgaaactttt gaaacgctgt tgcggacgtg gtcggttgca ggtcgcagtg
    21781 tacgtcctga tcatcgcatg gtcgcgcact cgggctttct caccgtggcc aggaaagtgg
    21841 aaccgggatt ctgcatgccc gttcaagatg tccaggcgac taatgacgag gacaggcatg
    21901 aggacgcgga cggaaaggac cagggacatg catcgtctgg agggtaaggt ggcgattgtc
    21961 accggcggca acgccggcat aggagaagcg gttgcaaaac gtttcgccga tgaaggagcc
    22021 gccgtcgtcg tgacgggcag aaggcaagcg gagttggagc gggtcatcag tggaatcagg
    22081 cacaacaagg gaaaggcatt ggcggtcgcc ggctcagtga ctgacgaagc gcatgtccag
    22141 gaggtggtgc agcgagcgct cgacagtttt gggcgcattg acatcctggt gaacaacgct
    22201 ggcatcggcg attttgggaa gcgacttcac gagacagacg attctgcctg ggccacggtg
    22261 attgatgtga atttgacggg cgtctttcgc atcacacggg ccgtcgttcc tcacatgctg
    22321 aaacagggac gaggagccat tatcaacatt tcgtcggttg ccagcctggt tgggatccca
    22381 ggagcgccgg cttatgcggc atcaaaaggc ggggttgatg ccctcacccg cgccatggcg
    22441 attgaatatg cccaggatgg catccgttgc aacgtggtta atcccggtct tgtcgatacc
    22501 ccgatggccg ctccactgat ggcgaatcct gacatgctgc gtccgatttt ggctcactat
    22561 gccattcgaa ggccggggac ttcggaggag gtcgcaaaca tggtgttgta cctgtcttct
    22621 gatgaagccg ggtgggtcac aggagggacg ttcacgattg acgggggcat gacagtctcc
    22681 aagggatgac cgatctacgc tgacacgtca attcaggctc accagcgtca tggacatcaa
    22741 caccgccaca cagttttgcg gagtcattgg caatcccatc gagcactctc tctccccggc
    22801 tattcacaat gccgcgtttc agaagttggc cctcaatttc gtctatctgg catggaaggt
    22861 ggatagaatt ggtgatgccg ttcgcggcct ccgcgcctta gggaattttc gcggcgccag
    22921 cgtcaccatc cctcacaaag tcgcggttct accctttctc gatgaagtcg acacgacggc
    22981 gcgtcacatt ggcgccgtca acacgattgt ggctgaagaa ggacagttgc tcggcatgaa
    23041 cacggacgcg actggggcgc tccgtgcgtt aagggaaggg aacgctccat tgaaaggcgc
    23101 atccgtcgtc attgtcggct cgggtggcgc agcgcgtgcg attgcatttg cccttgcggc
    23161 cgaggctgag gtgcgtcgct tgcacttgtt gggaatcgag gtggatgagc gtaggaatct
    23221 tgcgacggat ttgagggaca a
//
LOCUS       DASSCQ010000073         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_271835, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000073 DASSCQ010000000
VERSION     DASSCQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271835"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..569
                     /locus_tag="VFH05_04870"
     CDS             105..569
                     /locus_tag="VFH05_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677575.1"
                     /translation="MEARLDAAEKYERGVALRKAGLFKQAIQEFEQAAEDRTYALKAH
                     AQIGLCHKSLGRHEEAVVSFRNALKSPQSSTKETVQILYVLGRSLEMLGRTGETLEVY
                     RWIRREDPAYRDVEFRIQQLSVRRTSSVRSRPPASWVGGILRSWQVLLGLPK"
     gene            complement(573..>2062)
                     /locus_tag="VFH05_04875"
     CDS             complement(573..>2062)
                     /locus_tag="VFH05_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6677576.1"
                     /translation="AHGYTAFGSTGLTASRLGFGGYRVDESEPEHRRALMRALEIGVN
                     VIDTSTNYMDGGSERMIGTVLREIVHTRAISRDEMIVVSKIGYVQGQNLKEAEARERT
                     GRPYPDMVKYGDGIWHCIHPEFLADQLDGSLDRLGLATVDVCLLHNPEYALSEAARSG
                     AASLLESRERFYRRIQQAFAYFESQVVAGRIQWYGVSSNTVGRDKEDPEAVSLFEVLS
                     AAARAAASVGMTRHHFAVLQCPMNVFESDVATRPNTGPQHDQSVLQFAQHEQIAVLVN
                     RPLNAMPGAGAGMVRLAECPLEGESGNLEEAFRAVEVLEDEYRSTLAAVIPYGGKGLE
                     PKDYFNWAKELAPLRTRLQGVEHWEQVEHQMIAPHVNQVLQAIPRLLRGTSAEQQWES
                     WRDRYVPRLLALLGLLRRTAAERSRVRTQQIAASIDPLLPESKRMESLSRKALWVIAS
                     TPGVTCVLNGMRTGQYVEDSTAILGWNQLSDPEAVYKAIAHLSAV"
BASE COUNT          449 a          632 c          556 g          425 t
ORIGIN      
        1 cccgttgacc cataaagatt ggttattggc catttgtggt ccgtcttttg ctcaaatccg
       61 gaatggaagg cacaactttc accacccgcc atgcgtaagg acccatggaa gccaggctag
      121 atgccgctga aaaatatgaa cggggagtgg cgctgagaaa agcagggttg ttcaaacagg
      181 ccatccaaga atttgaacag gcggcagaag accggaccta tgcgcttaaa gcacacgcgc
      241 agatcggcct gtgccacaaa tcactgggac gacacgaaga ggcggtggtg tcctttcgca
      301 acgcgctgaa atcgcctcaa tcatcaacga aggaaaccgt ccagattctt tacgtcctcg
      361 gtcgctcgct ggaaatgctg ggacgcactg gtgaaaccct ggaagtgtat cggtggatca
      421 gacgtgaaga tccggcgtac cgggatgtcg aattcaggat ccaacagttg agcgtaagac
      481 ggacctcctc cgtcaggagc cgaccgcctg cgtcctgggt cggagggatt ctcagatcat
      541 ggcaagtgtt gttaggcctg ccgaaatagc cgttacaccg ccgagagatg tgcgatcgcc
      601 ttgtacaccg cctccgggtc gctcaactga ttccatccca atatcgcagt cgagtcttcc
      661 acatactgac cagtcctcat gccgttcagc acgcaggtga ctccgggcgt gctcgcaatc
      721 acccacaagg cttttcgtga tagagactcc attcgtttgg attcaggaag aagcggatcg
      781 atcgaggcgg ctatctgttg cgttcggacg cgactcctct cggccgccgt tcgacgaagc
      841 aatcccagga gtgcgagaag tcggggaacg taccggtctc tccagctttc ccactgttgc
      901 tcggcagacg tcccgcgaag cagccgtgga atcgcctgca aaacttggtt cacatgcggc
      961 gcgatcatct gatgctcaac ctgttcccaa tgctccacgc cctgcaaacg ggtgcggaga
     1021 ggcgctaact ctttggccca attaaaatag tccttgggct ccaggccttt tccgccgtac
     1081 ggaatgaccg cagcgagcgt cgaccgatac tcgtcttcta atacctcgac cgctcggaat
     1141 gcctcctcca ggtttcctga ttcaccttcc agcggacact ccgccaagcg caccatgccc
     1201 gcccctgcac cgggcatggc attcaggggg cgattcacca gcacggcgat ctgttcgtgc
     1261 tgggcgaatt gcaggacgct ctgatcatgc tgaggccccg tattcggccg tgtggctaca
     1321 tcgctctcga atacgttcat gggacattgc aaaacggcaa agtgatgccg cgtcattccc
     1381 actgaggcgg ccgcgcgcgc cgctgcagac aacacctcaa acaaggagac ggcttcagga
     1441 tcctccttgt cacgaccgac cgtgtttgaa gaaacgccgt accattgaat gcggcccgcc
     1501 accacttgcg actcgaaata ggcgaatgct tgttgaatcc gccggtagaa gcgctcacgg
     1561 gactccagca ggctcgccgc gccgcttcgg gctgcttccg agagagcata ctccggattg
     1621 tgtaacaggc agacatccac ggtcgccaag cccaaacggt cgagcgagcc atcgagttgg
     1681 tccgcgagaa actccggatg aatgcaatgc caaatcccgt ccccatactt gaccatgtcg
     1741 gggtaggggc gccccgtccg ttcccgcgcc tctgcctcct tgagattctg gccctgaaca
     1801 tagccgattt tggacacaac gatcatttcg tcccttgata tggcgcgcgt atgcacaatc
     1861 tcgcgcagaa ccgttccaat catgcgctcg cttccgccat ccatgtagtt cgtagaggtg
     1921 tcgatcacat tcacgccgat ttccagcgca cgcatcagcg cccgtcgatg ttcaggctcg
     1981 ctctcgtcga cacgataccc tccgaagccc agtctcgaag cggttaaccc ggtcgatccg
     2041 aatgccgtat agccgtgtgc aa
//
LOCUS       DASSCQ010000074        32492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1723588, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000074 DASSCQ010000000
VERSION     DASSCQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 32492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32492
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723588"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFH05_04880"
     CDS             <1..519
                     /locus_tag="VFH05_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677577.1"
                     /translation="MIKAAPAAVAIERTGTEEDLRKQLRVKNGVTELLPARALFSQAP
                     TGTYGFIAPQPLGMALVTQSPDLMLERSAPSSNAYEIHKLADGSGMVVGFIDKALIPQ
                     VTAESRPHHIRIALSSNGSDKTPYIAAVPLTKLMVDRMPGRLDVKNPNSPTVLEMDLQ
                     GTANRKSVPGLQ"
     gene            complement(537..1736)
                     /locus_tag="VFH05_04885"
     CDS             complement(537..1736)
                     /locus_tag="VFH05_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6677578.1"
                     /translation="MKGLSIVLAGFITGTLLITSSFRPAHALPASGSSPSLSPKTETV
                     KKYLESCRNRSAQDAGCEKLRKDAVEILQEDLRTLGSTADRGYLLSIVPVFQSEEVEL
                     RIAAADAIGMIGPQDRDAEFLVPVTNDPVPDVRVAVGQAISRGKGPALTLLAQRVRSG
                     NKTGRTPEIPPDASQFTMPVVPNSIYLFDSSSPELGRVSYLTRRGDAAGFFKGKAKKG
                     PFKLEEFQEKFRYQLQDEHQAFNQAQDAASKQLEREKPDPKNMQAFAEYMGRIVSTQT
                     LSMTRTSLDLYQPDFYSLPTVYVLEERQIGQRTYPTRYVVTYQDQALRQPGYRLVWMT
                     VPDDAIKSAQAASLKEEQQEAGRKKEEEAVHKRQEALDDLIKKKDDADKKKFKKGQAD
                     LEKELGF"
     gene            complement(1774..2541)
                     /locus_tag="VFH05_04890"
     CDS             complement(1774..2541)
                     /locus_tag="VFH05_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677579.1"
                     /translation="MSYGLWRGHNLFEAMRHRRPSQESGVMARALSVTSLCGALLLIV
                     FHTTAMGAGPCTCDDIDKMQKHLDRVNDAGEAWKEIFAWARELYRDVDMPQSNDDLNQ
                     KFVQLTSAQKSQWYDLIKQGPVKEKKTIKKVAGVSPEGEPVVDDDFKKSHCDDIIEAE
                     HVHERAHKDFYLSFPKVLEVPMNSRLLRLRAESEVESYRAHKAFLEKKLAELKLKCVT
                     KLDNSKKRELEQAAAQRQRMNEAEIRVRLLGTAQSGK"
     gene            2599..3996
                     /locus_tag="VFH05_04895"
     CDS             2599..3996
                     /locus_tag="VFH05_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677580.1"
                     /translation="MRSAFARQNSGLLWLVLLAVLVYANAWSGGFQFDDFSAILDNPH
                     LADVGTFFGHLDHMVRPVLYATLLFDRTLYGMNPTGYHLCNLLLHLGSGMLIYFILTR
                     AVTEKTRQVPFWTALLFLVHPIHTETVTYVSGRASGLMSFLYLLALFFYIKASEAPTM
                     RGLYRSGAIVAFVLSIGAKETAITFPFVLLLWDLLVHRVRGGASLRAAILRRHLPFWV
                     VLLLTAAWAWSHPRYFILAHVSYTLRPLVDNGLSEAHAAIYALWLFFCPWNQNFDHDL
                     PEFHSMGQWPLPLDLVALGALIAVAVGMARRLPLVSFGIGWFLLQLLPTSLIPRADLL
                     SERNLYLASIGLLLALVALAATFARIDEIAKHRLLVRSVGASMACVLVVGLCLMTVQR
                     NTLYRDQLSLWSDAVKKSPNKARPHNNLGHAYALHGEWDRAIDEFRIAAQLDSDYVLA
                     QENLRDAYLHRVGRR"
     gene            complement(4016..4864)
                     /locus_tag="VFH05_04900"
     CDS             complement(4016..4864)
                     /locus_tag="VFH05_04900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET6677581.1"
                     /translation="MDRRLRGRALVIAWVMIAHALWPAAGLTDNSLPHLPERPVIKAA
                     AVYLVELKSGRVLLEKNATRRLPPASLTKIMTALIALESAPLQDVVKIDQRAVAHHSS
                     YSFRPGEEFLLRDLVTAMLVVSANDACEAVAWHVGGDDQRFVSMMNERALLWGLENTH
                     FSNPCGFDAPEHYSTAADLAKLTERALQEPIFSMMVRTVVRDISTVNGTRRMSLHSTN
                     ELLADPEVNGVKTGYTSRAGRCLIATMFKDGHRLMLVGLNLRDQWKEASRLLRYGHDV
                     LQGASD"
     gene            complement(4877..5668)
                     /locus_tag="VFH05_04905"
     CDS             complement(4877..5668)
                     /locus_tag="VFH05_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HET6677582.1"
                     /translation="MPHILLVLVALATLSTWLPAQAAPMNRPQAEYSADATIENEEGA
                     TTQKIFVTPTKERKEMLTGAGDGAVQIFRYDTKVMWMLMPSENMYMEYSIGGGPSKGN
                     DTSQWTYEDTAMGEETLNGVKVTKYKTIATSTDGKKFGGFSWRTKEGINVKQDLLYKE
                     GNDKKRMMTELSNLRIGRQDPKLFEIPDGFTKLDMGGMMGMMGRQGQSMGRPDMSGTP
                     PPSARKPGSRPEVVVPQETPAPEPEAAPESEVDKATNFMKGLFGH"
     gene            complement(5819..8500)
                     /locus_tag="VFH05_04910"
     CDS             complement(5819..8500)
                     /locus_tag="VFH05_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HET6677583.1"
                     /translation="MASRRQPTAKARPPVTAEEKRLQEDARREQHWKRWGPYLSERAW
                     GTVREDYSPSGTAWEAFPHEHARSRVYRWNEDGLAGLCDRHQYICFALALWNGHDPIL
                     KERLFGLTGHEGNHGEDVKEYYFYLDSTPTHSYMKFLYKYPQSTFPYERLVEENRRRS
                     RHEPEFELHESGCFDAQRYFDVFVEYAKASPEDICIRVEVINRGPDPAELTVLPTIWY
                     RNTWSWGSDIRRPRFQAGVPIDAASVIETVHDYYGVRRLLCDKQPPLLFTENETNSRR
                     LYADDEGPRYAKDAFHDYVVWGLKAAINPDHIGSKAAAHYALTVAAGAAGRINLRFTN
                     DAHVAGFTRQNFEEVFERRIQEADEFYETLAPRRLSKDARRVQRQAFAGLLLSKQYYH
                     YDVSRWLKGDPMGPEPPPNRLHGRNADWTHLYNADVLSMPDKWEYPWYAAWDLAFHCI
                     PLALVDPTFAKEQLILMLREWYMHPNGQMPAYEWALGDVNPPVHAWAAWRVYKIEQKR
                     RGVGDRVFLERVFHKLLLNFTWWVNRKDTEGKNIFQGGFLGLDNIGVFDRSAPLPTGG
                     RLEQSDATSWMGMYCLNMLTIALELAGENRAYEDVASKFFEHFVYICRAVNNIGGTRI
                     ELWDGTDGFFYDVLHLPNGETTHMKVRSMVGLIPLFAVETLDSTLIDRLPRFKHRMQW
                     FIENRPEFAQHVETQSYEGGVRRFLSLVNRHRLRSIMRYLLDENEFLSPYGIRALSRH
                     HRDHPYVLNVMGHEHRVDYEPAESSTPQFGGNSNWRGPIWFPVNYLLIESLQKFHFFL
                     GDHYKVEYPFGSGQQATLWQVAGELSRRLIGIFLRNAEGKRPVFGGAQMFQQGPHWQD
                     HLLFYEYFHGDNGAGIGASHQTGWTGLVAKLIQQSGK"
     gene            complement(8522..10267)
                     /locus_tag="VFH05_04915"
     CDS             complement(8522..10267)
                     /locus_tag="VFH05_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6677584.1"
                     /translation="MSTFARFRPFLAPYLGRMVLATVLVMAVAAINLALLRLAGALWD
                     VITVQHDGARMNELILLFLSLVFLQGFCSMGHSYLTASVSQRIVADFRRHLFAHLQTL
                     SVSFFARRRTGELLSRLMNDVTVIQSVVTETPIDTAKQLVTFVGGITFLLAMNWRLCL
                     LILVLLPLLVVVAKLFGRRLKSLSTSIQDQTAALSTLLEEVISGIRIVKSFVKTQREE
                     ARFAQQVDTTLSLTMRRAGIMAVFIPVISLLTFSSAAAVLWYGGRQVIDGTVSPGELF
                     AFVLFAGILIGPFSSAARVFAQIREAQGATERVFEILDTRPEVRDDPNALVLHTVSGQ
                     LTAEHVSFAYDPRRAILIDVSFEAKPGECVAIVGPTGAGKTTLMNLIHRFYDPTEGRV
                     TIDGTSLRDVTLESWYRQIALVPQETILFGGTILDNIRYGNGDADEEAVKEASRAANA
                     HEFIMGFPDGYHTVVGEKGINISGGQRQRIAIARALLKNPRILLLDEATSALDSESER
                     LIQEALERLMRGRTTFVIAHRLTTIHSADRILVLDKGRLVETGTHDELMARQGLYHYL
                     YTVRVVEAPSKTAGV"
     gene            complement(10364..10582)
                     /locus_tag="VFH05_04920"
     CDS             complement(10364..10582)
                     /locus_tag="VFH05_04920"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677585.1"
                     /translation="MPEGKKVRIRVRTVNCVYVGDFLIPPMRNRVSDAINEEQRLFIS
                     LTDVVVNDKDRSDFVAINKNLIESIAQL"
     gene            10833..11336
                     /locus_tag="VFH05_04925"
     CDS             10833..11336
                     /locus_tag="VFH05_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677586.1"
                     /translation="MHSSRHQVTALAIVLVLWMVGTGCGSKTMQYPEDHERYLRIDKA
                     VESLRVAYVKKDSAGIAALVVPTESTERLQQEAEADFELFHDMAMDFHVERIMIDNDD
                     IDVYVHWQGLWKKDPEDPGIRHRGHSRLQWVGTKSVLLSGIQGDIPFGVNRRQALTGQ
                     APGVGRK"
     gene            11339..12982
                     /gene="nadB"
                     /locus_tag="VFH05_04930"
     CDS             11339..12982
                     /gene="nadB"
                     /locus_tag="VFH05_04930"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET6677587.1"
                     /translation="MPANRLSSLLSADFLVIGSGVAGLRAAVELSRNGQVVVITKGHP
                     LQSSSIHAQGGVAVALSEEDDVAIHLTDTLKAGHGLCRKEAVRVLVGEGPQRIQELIK
                     WGARFDKAGGKFAFAREAAHSRSRILRARGDATGNEMVRVLIAEAMRQKRIQRLDYHF
                     TVDLIVESGRCCGALVFDEHSGRRFILPAKAVVLTTGGAGQLYARTTNPANATGDGIA
                     MALRAGAVLQDMEFVQFHPTALYLPSSPPFLLSEAMRGEGAQLRNNKGEIFMPRYHPL
                     GALAPRDIVSRAIWAEMAATKARHVYLDVTHLGAEFVKRRFPTIYATCLRYDIDITEE
                     WIPVSPSAHYMMGGVWTDLNGATTVPGLFAAGEVACSGVHGANRLASNSLLEGLVFGA
                     RAATAAVAYAGREEIPSLATHEAALKHEQYGSLDDVEKLRSSLRRTMWGQVGVIRSGE
                     SLIRACAQLSRWAQLVAQPFSQRTALEVKNMVQVAQCIAEAALWRENSVGAHYRSDFP
                     QAKRPGWQQHSRLSLGEGISGTPPRKSRGLLLALKGRAG"
     gene            complement(12918..13616)
                     /locus_tag="VFH05_04935"
     CDS             complement(12918..13616)
                     /locus_tag="VFH05_04935"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6677588.1"
                     /translation="MTATLRRITSLIRCSSGMLLTGVIVLSPLNPVGSQAEIYQYIGP
                     NGTISLTNVPSDARYRKLDVESARFHALLSERELEPVIKRHSSQQQLHPALIRAVIKA
                     ESNFDPRAVSRAGAIGLMQLMPQTAVRLDVRDMFDPDENVGGGAKYLRQLLDRFHGNL
                     PLALAAYNAGENIVDRYQALPPIDETRQYVRKVLRYYRTFLVRDGVITERPVSRHAPS
                     TPAEAPATSVEASR"
     gene            complement(13729..14604)
                     /locus_tag="VFH05_04940"
     CDS             complement(13729..14604)
                     /locus_tag="VFH05_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET6677589.1"
                     /translation="MGWIYLHDRFVKEEEALVSVFDHGFLYGDGVYETIRSYGHKIFM
                     RDQHLARLRRSADGIGLSIPQLNWPSVLHEAMARNGVGDEHTDAYIRVTISRGVGEIG
                     LDPALCPTPTIVIMAKPLKPLPQDCYRTGVSLIVAKTRRNLPSALDPQIKATNFLNNI
                     QAKREALNAGAFDSVLLNWESHLTECTVSNLFFVRSGRLCTPALSCGILDGITRGVLL
                     GLARESAIPVEEGQFTVESLQTAEECFLSNTTMEVMPATQLNGRPIGNGTPGPLSKRL
                     HQIFVTHRDKFLELA"
     gene            complement(14604..16154)
                     /locus_tag="VFH05_04945"
     CDS             complement(14604..16154)
                     /locus_tag="VFH05_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET6677590.1"
                     /translation="MNLSSSGCWEITESVGYSELLLREPDSNHDPIMTRFSQPSFLQG
                     APRPLVLSFKGRGESPFDLYQKVASSTQPSFLLESGKGENRNYSFFGSHPASVMPRPS
                     LATASSSDDPFSWLRHIIASPGIERTPDLPPFFGGAVGCFSYDFARRLESVPAVAVDD
                     LSLPDMHFALYEVVAAVDHRTNRIQVIFCPSLDRFLGEARDKLYREGLDRLAEWEARL
                     TGSVATPPSPLALTTVSFGPQQTQEAYLDRVRRCQEYIAAGDIYQANLSHRFGIEIPQ
                     GYRSGAARLQYEQALYQELRSVNPSPFSGLLRFGDMSLISCSPERLVRLQGRRADTRP
                     IAGTRPRGCNSDDDRRLVRELLANEKERAEHVMLVDLERNDLGRVCEIGSVQVNEFMV
                     VEQYSHVSHIVSNVQGTLRPGATPFDLISATFPGGTITGVPKIRCMEIIEELEPFRRG
                     PYTGSFGYIGWNGDLDLNIIIRTLVMTEKQGYLQVGAGIVADSDPAKEYQETLQKAQA
                     FFSAVGRR"
     gene            16233..16451
                     /locus_tag="VFH05_04950"
     CDS             16233..16451
                     /locus_tag="VFH05_04950"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET6677591.1"
                     /translation="MPQSQDPFYAGLGQAVRIGTELLAALIVGGGLGWAVDTYLLETG
                     PWGLVLGLILGAAAGVRNAYRMAQRWPQ"
     gene            16478..17227
                     /locus_tag="VFH05_04955"
     CDS             16478..17227
                     /locus_tag="VFH05_04955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET6677592.1"
                     /translation="MEESPLHPFELHNYIPINLGGLDISINKAVIMMWVVVALISILM
                     VTAGSARRLVPGKLQSLAEMLVDFIRGIILDTMGKDGMKFFPLIATLFLFILFCNLIG
                     LIPGSYTVTSQIVVTAVFACTVYGLSLLMGFVLHGGKFFGILVPPGTPGWLLPLMVPI
                     ELISQLARPISLAVRLFANMTAGHVILGVLFGLAVSGGVLIGWLPFAFTIAMNGLEVG
                     IAFIQAYIFTVLTCVYLGDAFHLHGHDEHAH"
     gene            17310..17540
                     /gene="atpE"
                     /locus_tag="VFH05_04960"
     CDS             17310..17540
                     /gene="atpE"
                     /locus_tag="VFH05_04960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET6677593.1"
                     /translation="MDSAAAALLGMGLAAAGFAGAGVGIGYIFGKMIEAVARQPEAEG
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGLRK"
     gene            17693..18196
                     /gene="atpF"
                     /locus_tag="VFH05_04965"
     CDS             17693..18196
                     /gene="atpF"
                     /locus_tag="VFH05_04965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET6677594.1"
                     /translation="MPQFESHFFSSLIFWEVVSFAILLFVLYKFAFPGILSVLEEREK
                     KIKNSLDEAEAHRVEAERRLKEYETRLAGASREAEGILAAAKERTQRLMEENEQRLTA
                     EAERIKGDATREIEHERRKAIQEIRTQTTDLALMVAEKVVQRSLTDADHRRLADEALD
                     ALSRSSH"
     gene            complement(18269..19078)
                     /gene="larE"
                     /locus_tag="VFH05_04970"
     CDS             complement(18269..19078)
                     /gene="larE"
                     /locus_tag="VFH05_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET6677595.1"
                     /translation="MTMDALTDKVERLRHLVADMQSALIAFSGGIDSTLVLKIAHDEL
                     GARAVGVTAVSPTYPAAELDAATRTAQDIGAEHAIVQTDQLTIPAFVQNDGARCFHCK
                     TDLYQLMERLRIARSMQWVLDGTNLDDLGDDRPGIKAAREWNVRSPLVEAALTKADVR
                     AAAHQLGLSSWDKPAAACLSSRIPRGIPITMEKLRRVEAAEAVLQEEGFSHFRVRDHG
                     DIARIEMAPEEFARLNQPGRRLTVSTRLREIGFRFVCVDLEGYRPGGISLA"
     gene            complement(19090..20463)
                     /locus_tag="VFH05_04975"
     CDS             complement(19090..20463)
                     /locus_tag="VFH05_04975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6677596.1"
                     /translation="MIRTRLHPMLRRGLQWCLGTSVFTLVFATFPVSPSGAAEIVPTK
                     FVAPNGLTVVVLEQHFLPIVEVHALVKSGSAQDPPEKAGLANLVASLLDEGTTSRTSK
                     QLAEQIDFVGGALEVKAAEDFTTASVRVLKKDVELGFTLLADILQHPAFLKPEFERIR
                     SQILGEMASDNDDPGHVAMKAFNQLVFHGHPYRWPLNGTEESLNKITHADVVGFYARE
                     YAPSQMILTIVGDVTAEQVTTFVQTHFGGWKKASQPPRSAKSAPPIEKKTVQLIEKDL
                     TQSNVILGHGGISRTNPDFYAVTVMNYILGAGGFSSRLMDSIRDKQGLVYGIMSHFDA
                     RLMPGSFWVNFQTRTEATNQAIQGVLAELKSIREAPVTDQELSEAKSFLIGSFPLRLD
                     STAKLAQVLAQVEFYGLGFEYFSQYPKWIERVTKEDVQRVAKQYLYPQRYALVVVGNV
                     AKAKLKQ"
     gene            complement(20460..21833)
                     /locus_tag="VFH05_04980"
     CDS             complement(20460..21833)
                     /locus_tag="VFH05_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6677597.1"
                     /translation="MIRLRFPHIPFWLLTISALAVSVQAAAAEPHEYLLSNGMKVLLV
                     EMPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTAKYPKGSFSRLIRKNGGM
                     DNAFTSQDFTAYFENLAADRVQLALEMEADRMQGLILDQNELKTEREVVKEERRLRTE
                     DDPQGALVEALFAQAYLSHPYHWPVIGWFGDLDAMTLDDLQRHYDTYYSPNNATLIVV
                     GDIKAEQLLPTIKHLFEPIPRGPEPKPVATMEAEQKGERRFLLKREAQVPFVMMGYRV
                     PNYTSEDSYALDVLESILSHGKSSRLYQSLVYDQKLSLAVGAEYGLMQTDPGLFYFYA
                     LVSPGQKVEVVEEALHKEIKRLQSEAPTEQELQRAKNQVEAARIFEQDSNFRHAMLLG
                     QAESVGAGWRKVDQFLERIRAVTAKDVQRVAGQYLLEDSRTVGSLIPVTPKPAEPPTA
                     SVQQGKP"
     gene            complement(21864..22961)
                     /gene="rlmN"
                     /locus_tag="VFH05_04985"
     CDS             complement(21864..22961)
                     /gene="rlmN"
                     /locus_tag="VFH05_04985"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET6677598.1"
                     /translation="MRGVKTMTPPTTMNLLALDESETAALVERLGWPAYRTGQILRWL
                     YQRRARTISQMTDLSQADRATLEREARIGRSLGCTVFRSADGTRKLILKLDDELEVEA
                     VLIPDDERLTLCVSTQVGCTLDCGFCLTGTMGLKRNLRAHEIIEQVLTAHDHLDPGER
                     LSNMVFMGMGEPLANLAALSEAVCRLTNPKWGLGWSPRRITVSTAGFASKLHQVAALG
                     VNLAVSLNATTEEQRARLMPAVHGIASLKTLLSACRRYPLPRHRRLTFEYVLLAGIND
                     GEADAKRLVTLIKGINCKVNLIPFNPFPGSPFRRPSDRHILTFQSIVRQAGMDVFIRK
                     SRGRDVLGACGQLGNLPPSYERTLTQIESHC"
     gene            23084..25309
                     /locus_tag="VFH05_04990"
     CDS             23084..25309
                     /locus_tag="VFH05_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6677599.1"
                     /translation="MVAVLVRCCACALLLLITGAAGSAALPGESDPCEQPELCFQAAA
                     FPKERLGKVMNKEQVLTLKLERLQQLADRFSGTLWGKRAGLLSGVLLIERDPASAMQF
                     LRGAQRDFPVLDDYIRVWIAESQLNLGQAKEAAVLLESIPMAVPDSNILGKAAYKTGE
                     AWYQAAACAESTAWFGRAVAMNDKDASAPQAYLRRGACRLRDNNVVEGRELLQQVWVR
                     FPYTREAKEAETLLTSNLGGDTWTVQPVDRLNRAQAFLGQAFHAEAIEELKKFLLADP
                     HSPRRAEAKLKLGIAQVRLKLYDHARETFRGLAKEGGPESREAAVWLARVYLRQGQGD
                     KLLEAARAISSSSLSAEQKGQITLFAGMWLEDQKQFDDAIAKYVHVAHHGEPTAQRAE
                     GQWRVGWVYYRTARYREAGESLRALAEQHDSEFAPQALYWIGRAGELNNQPLEAQDFY
                     RKLCQRHPFTYYCQLARERLNIPPIADAPIETLVLSSDTAKTGDPARPPVTQADIEQH
                     TAYRRAIELKTLGLDADAAHEVAVLTDRYSRDPDVLIALSTMLNEVGAYHQALRLARA
                     RFRDQLERTGGAFAPSLWNVAYPTGLLPTIKLQAAKGVDPYLIAAIIREESQYDVKAV
                     SRVGAIGLMQVMPATANNVAQRVGLPAVGREDLFDQETNIRIGVRYVEQLLEQFSGNL
                     VYTVASYNAGPLAVGNWIAQYRGHNQDEFVELIPYQETRQYVKRVLRSYREYVRLNGL
                     S"
     gene            25384..25638
                     /gene="zapB"
                     /locus_tag="VFH05_04995"
     CDS             25384..25638
                     /gene="zapB"
                     /locus_tag="VFH05_04995"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HET6677600.1"
                     /translation="MALDRLDALETRIRDLVKLIQELKKRNAGLEDDLRSTRQRLADE
                     SDSNRRWARERSDIKARVEKVLGDVELLEAFEERKEVAFD"
     gene            25631..25927
                     /locus_tag="VFH05_05000"
     CDS             25631..25927
                     /locus_tag="VFH05_05000"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET6677601.1"
                     /translation="MTKTTDVEIYGQRYSIKGEADETYIRRLAGFVDGQMRQLAEGMN
                     TTTPSRLAVLTALNLAHQLLEAEKKRAQGEADVERRMTSLMESIEEQVPTSLFR"
     gene            26502..28070
                     /gene="rny"
                     /locus_tag="VFH05_05005"
     CDS             26502..28070
                     /gene="rny"
                     /locus_tag="VFH05_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET6677602.1"
                     /translation="MSTSIVMYILVGLVGAFLGAGLFELVRRSSVTAKRAQEADQARQ
                     TIQNAQREAENIVKEAKLEAKDLVFQSKAELEKEQKAKLLELSMTEKRLVQREEAIDK
                     RISALDKRDADGQKRDQDLIRKEEKLIAKEAACAQAEREHREALERVAGMTADEAKRH
                     LLQEMESQARLDAAGIAKRTLEEARENAEREAREIITTSIQRVVRDYVSESTISVVPI
                     PNDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIISGFDPLRREIAKVSLERLM
                     HDGRIHPTRIEEIVEKVKVDIDKLMYEEAEKIIFELGLSDFHPELIKVLGRLKYRTSY
                     GQNNLYHAREASYICGIMASELGLDVRLARRGALLHDIGKAVSHEEEGPHAMLGAEIA
                     KKYGESEKIVNAIAAHHEQVEPICPESVLVAAAEALSAARPGARREALESYVKRLEKL
                     ESLAHAQKGVQKAYAIQAGREIRVIVRQEDVTDSESFQLSRELAKKIEQELTYPGQIK
                     VTVIRESRYIEYAK"
     gene            28067..28846
                     /locus_tag="VFH05_05010"
     CDS             28067..28846
                     /locus_tag="VFH05_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET6677603.1"
                     /translation="MKVLYIGDIMGEPGRRAVARAVPRLVSQRQIDVVVGNGENVAGG
                     FGITPDLADELFGIGLSAITTGNHAWDKKEILDYFPRERRLLRPANYPEGVPGQGSVV
                     IRTKSGEEVAVLQLMGRAYMPTLDCPFQVAKREMSRLRKLVAAVIVDMHAEATSEKMA
                     MGHYLDGEATAVVGTHTHVQTADEQILPRGTAYLTDIGMTGPLHGVIGVKKELAIEKF
                     LTGMPRRFEVAGGPTVFCAALIELDARIGKALSIERIRFTD"
     gene            28848..30221
                     /gene="xseA"
                     /locus_tag="VFH05_05015"
     CDS             28848..30221
                     /gene="xseA"
                     /locus_tag="VFH05_05015"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6677604.1"
                     /translation="MSATRQQTLFHPSKSVLTVSQLTGLLRTTVEGQFSDVWLEGEVS
                     NLRMPGSGHIYCTLKDESSQIRAVLFRSSALRLKFTLQEGMCIIVRGRLTVYEPRGEY
                     QIVLETVEPKGIGALQLAFEQLKARLAAEGLFDEARKKPLPTFPSTIGVVTSPTGAAI
                     RDMLSVLHRRWPILHIILVPVPVQGEGAGQHVADAVALLNEQAVVDVIIVGRGGGSME
                     DLWSFNEEVVVRAVAASRIPIVSAVGHETDVTLTDFAADRRAATPSAAAETVVPVLAD
                     VVERLRVLTVRNGHAMARYCVLEQRRLDAYISGLAQVQLRIQREWQRTDGTIGRLKTL
                     VCERVAVSRDLVRQHQRELAGLNPIFYVKRGLSLIPTLVHRLERQILVLSEARKRRIA
                     AMAAQLTQLSPLAILGRGYSILMRARDGTILRSALDAEVDEELVARLAQGELDCTVKR
                     VVPDPLV"
     gene            30278..30538
                     /gene="xseB"
                     /locus_tag="VFH05_05020"
     CDS             30278..30538
                     /gene="xseB"
                     /locus_tag="VFH05_05020"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6677605.1"
                     /translation="MGGVKFEQAMARLEAIVGELEKGDLPLDESLKIFEEGIRLSKSC
                     LKILEDAERKVEVLVQDRNGKKRLHAFSPDEEAEESADESSC"
     gene            30604..31371
                     /locus_tag="VFH05_05025"
     CDS             30604..31371
                     /locus_tag="VFH05_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET6677606.1"
                     /translation="MSSKAPVAKERLDRLLVSQGLSTSREIAVRTILAGGVRVDGTVV
                     DKPARLVPRGARIEIERPAPFVSRSGEKLAAALDAFSIDSRGRVGLDVGCSTGGFTDC
                     LLQRGIKRVYAVDVGYGLIDWKLRQDSRVILLERTNIRYVDRSLIPEPIDLAVIDVSF
                     ISLKLVLPSVVPLLREDAHVIALVKPQFEVGKGQVGAGGIVRDDAQRRAVTERVIECA
                     AGLGLHHTGTLDSPVKGRKGNREILVSFLRRGIIASS"
     gene            31400..32323
                     /locus_tag="VFH05_05030"
     CDS             31400..32323
                     /locus_tag="VFH05_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6677607.1"
                     /translation="MKVLVTGGAGFIGSHLVDRLVMEGHEAVVVDNLITGKRRNVNRA
                     ARLYKMDIQSWRLERVFRNERPNVVMHLAAQMDVRKSVEDPMFDAQVNILGTLNVLQQ
                     AVKHGVRKVIFSSSGGAIYGEQETYPAPETHVLKPLSPYGLSKLCGEQYLSYFQRVSG
                     LQAVSLRYANVYGPRQDPEGEAGVVAIFIQKMLNNEQPVINGNGRQTRDFVFVDDVVE
                     ANLAMIGQETQGTYNVGTGVETSINDLFRILVQHTGSVCKEIHGPAKKGEQARSVIDN
                     TKLRQELSWEPRADLVDGLKKTVEYFQARVH"
     gene            complement(32362..32481)
                     /locus_tag="VFH05_05035"
     CDS             complement(32362..32481)
                     /locus_tag="VFH05_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677608.1"
                     /translation="MRSADATNFLLQQGFSNVKNLVGGIDAWSAQVDRSVPRY"
BASE COUNT         6765 a         8971 c         9649 g         7107 t
ORIGIN      
        1 atgatcaagg ccgcgcccgc ggcggtcgcg atcgagagaa ccggcactga agaggacctc
       61 cgcaaacaat tgcgtgtcaa aaacggtgtg actgagctcc tcccagcacg cgcgctcttc
      121 tcacaggctc cgactggaac ctatggcttt attgccccgc aaccgctcgg gatggccctc
      181 gtgacgcaga gtcctgatct catgctggag cgcagcgctc cttcttccaa cgcctacgag
      241 atccataaac tcgccgacgg cagcggcatg gttgtggggt tcatcgacaa ggcgctcatt
      301 ccgcaggtca cggcggaaag ccggcctcat catatccgga ttgctctgag ctccaacggt
      361 tcagacaaga cgccgtacat tgctgccgtg ccgttgacca aactgatggt cgatcgaatg
      421 ccgggtcggt tggatgtcaa gaatccaaat agtcctacgg tgctcgagat ggatctgcag
      481 gggacggcca accgtaagtc cgtaccaggt ctgcaatagc cggtgtcctg cgaggactaa
      541 aatcccaatt ccttttcaag gtcggcctgc cccttcttga acttcttctt gtccgcgtcg
      601 tcctttttct tgatcaggtc atccagagct tcttggcgtt tgtgaacggc ttcttcttcc
      661 tttttccgac cagcttcttg ttgctcttct ttcaatgacg cggcttgggc cgacttgatc
      721 gcgtcgtccg gcactgtcat ccacacaagt cgatatcccg gctgcctgag tgcttgatcc
      781 tgataggtga cgacgtagcg ggtggggtag gtgcgctgac cgatttgccg ctcttcaagc
      841 acgtaaacgg tcggcagact ataaaaatct ggctgataca aatccaaaga ggtcctcgtc
      901 atgctcaggg tctgcgtcga gacaatccgt cccatgtact cggcaaacgc ctgcatgttt
      961 ttgggatcag gtttctccct ttccagttgc ttactcgcgg catcctgcgc ttggttgaac
     1021 gcctggtgtt catcttgaag ctgatagcgg aatttttctt ggaactcttc cagcttgaag
     1081 ggacctttct tggccttgcc tttgaaaaat cccgctgcat cgccgcgcct ggtgagatag
     1141 gagacacgac cgagctcggg actgctcgaa tcaaagagat agatactgtt gggcacgacc
     1201 ggcattgtga actgactggc atcaggcggg atctccgggg tacggcccgt cttatttcct
     1261 gacctgacgc gctgtgccaa caacgtgagc gcaggtcctt tcccgcggct gatcgcctgc
     1321 ccaactgcta ctctcacatc aggaactgga tcgttggtaa cggggaccag gaactccgca
     1381 tcacggtctt gtggtccgat catgccgatg gcgtccgctg cggcaatccg caactcaact
     1441 tcttcgctct ggaagaccgg cacgatgctg agcaaataac ctcgatctgc cgtggacccg
     1501 agcgtacgca agtcttcttg cagaatttca acggcgtctt ttctgagttt ctcgcatccc
     1561 gcgtcttgag ccgatctgtt ccgacaactc tcgagatact tcttgacggt ttcggttttg
     1621 ggagaaagcg agggagagga tccggatgcg ggcagagcat gagccgggcg aaacgatgat
     1681 gtgatcagca acgttcctgt gatgaaccct gcaaggacga tcgagagacc cttcatggtt
     1741 gttcctccta acagtgtcga atctcggtgt ctgtcacttc ccagattgcg ccgttcccag
     1801 caagcggact cggatctccg cttcattcat tcgctgccgc tgtgccgctg cctgctccaa
     1861 ttctcttttc ttggaattgt cgagtttggt gacgcatttc agcttcagct cggcaagttt
     1921 cttctcaaga aacgccttgt gggcccggta ggattccact tcagactccg cacgtaatcg
     1981 aaggagccgg gagttcatag gcacttcaag gaccttcgga aaactcagat agaagtcttt
     2041 gtgcgctcgt tcatgaacgt gctccgcttc gatgatgtcg tcgcaatggc tttttttgaa
     2101 gtcatcgtcg acaacgggtt cgccttctgg gctgaccccc gcgactttct tgatggtttt
     2161 cttctccttg acggggcctt gcttgatgag gtcataccat tgagacttct gcgcgctggt
     2221 cagctggacg aatttttggt tcaagtcgtc gttggattgt ggcatgtcga catcccggta
     2281 cagctcccgc gcccaggcga agatttcttt ccaagcttct cccgcgtcgt taacccgatc
     2341 cagatgcttc tgcattttgt cgatatcgtc gcatgtgcag gggcctgctc ccatcgccgt
     2401 cgtatgaaag acgatcagca ggagcgcacc gcacagggac gtcacgctca gcgcacgcgc
     2461 catgaccccg gactcttgag acggccgtct atgccgcatc gcctcgaaca gattgtgccc
     2521 acgccaaagc ccataggaca tgatccttcc tcctctctgt gtactcatct gttagaagag
     2581 cgtattcttg tgccacccat gaggtctgcc ttcgcgcgtc agaattccgg actcctctgg
     2641 cttgttctcc tggctgtttt ggtatatgcg aatgcgtggt ctgggggttt ccaattcgac
     2701 gatttctccg ccatccttga caatcctcat ctcgccgatg tcggcacgtt tttcggccac
     2761 cttgatcaca tggttcgtcc cgtcctgtac gcgacattat tgtttgatcg taccttgtat
     2821 gggatgaatc ccactggata tcacctgtgt aatctgctgc tccatctcgg gtcggggatg
     2881 ctgatctatt tcattcttac ccgcgcggtc acggagaaaa cgcgacaggt gccgttctgg
     2941 actgcgctgt tgtttctcgt ccaccccatt cacactgaaa ccgtcaccta cgtttcaggg
     3001 cgggcctcgg gcctgatgtc ctttctctac ctcctggcac tcttcttcta catcaaggcg
     3061 tcggaggctc ccacaatgcg cggcttgtat cgatcaggcg ccatcgtcgc ctttgttctg
     3121 tcaatcggcg cgaaggaaac ggccatcacc tttccttttg tgcttctgct ctgggatctg
     3181 cttgtccatc gagtgagagg cggcgcgtca ctccgagcgg cgattctccg acggcatctc
     3241 ccgttctggg tcgtgctact gcttactgcg gcatgggcgt ggagccatcc tcgctacttc
     3301 atccttgcac atgtcagtta cacgttgcgc ccgctcgtgg ataacggctt gagcgaagcc
     3361 catgctgcta tctatgcgct gtggctgttc ttctgcccat ggaaccagaa cttcgaccat
     3421 gacttgcctg agtttcattc gatgggtcaa tggccgctcc ctctcgacct tgtcgctctc
     3481 ggggcactga tcgcggtcgc cgtcggcatg gctcgccgcc ttcccctggt atcgttcggt
     3541 atcggctggt tcttgcttca gctccttccc accagcctta ttccccgtgc cgatctttta
     3601 agcgaacgca acctctacct ggcgtcgatc gggttgttgc tggcgcttgt tgcactcgct
     3661 gccacgtttg cccggatcga cgaaatagcg aagcatcgtc tcctggtccg atctgtcggg
     3721 gcaagcatgg catgcgttct ggtggttgga ctctgcctga tgaccgtcca acggaacacc
     3781 ctctatcgag atcaactctc tctctggtcc gacgcggtga aaaaatctcc caacaaggcg
     3841 aggccgcaca acaacctggg gcatgcctat gcgctgcacg gagagtggga ccgagccatc
     3901 gacgaatttc gcattgccgc tcaattggat tccgactacg ttctcgcaca ggaaaatctt
     3961 cgtgatgcct atctgcatcg cgtgggccgc cggtagccat tacggtgctt ctgcttcagt
     4021 cgctcgctcc ctgcagaacg tcgtggccat agcgtaagag ccgtgaggcc tccttccact
     4081 gatccctgag attcagcccc accagcatca agcggtgccc atctttaaac atagtggcga
     4141 tgaggcagcg tcccgctctg cttgtatagc ctgttttcac gccgtttacc tcagggtcag
     4201 cgagcagttc gttcgtactg tgcagcgaca tgcgcctcgt gccgttcacc gtagagatgt
     4261 cccggaccac cgtccgcacc atcatggaga agatcggctc ttgcagcgct cgctcggtca
     4321 acttagccag atctgccgca gtcgaatagt gttccggcgc gtcgaatccg caagggttgg
     4381 aaaagtgcgt gttctctaat ccccaaagca gcgcgcgctc gttcatcatc gagacaaacc
     4441 gctggtcatc accaccgaca tgccaggcga cggcctcgca ggcatcattc gcgctgacca
     4501 ccagcatggc cgtcaccagg tcacgaagga ggaattcttc accgggccga aagctgtagg
     4561 aagaatgatg tgcgaccgcg cgctggtcga tcttcaccac gtcttggagg ggggccgatt
     4621 caagcgcgat cagcgcagtc atgattttcg tgagactggc aggcggaagc cggcgggtcg
     4681 catttttttc gagtagaaca cgcccggact tgagttcgac caggtacaca gcggccgcct
     4741 tgatgaccgg ccgttctggc aaatggggga gagagttgtc agtgagtccg gcagcaggcc
     4801 acagcgcgtg ggctatcatc acccacgcga tcaccagcgc tctgccgcgc agccgtctgt
     4861 ccatcccgtc gttcgcctag tgaccaaaca atcccttcat gaaattggtg gccttgtcga
     4921 cctctgattc cggcgccgct tcaggctccg gcgccggagt ctcttgtggc acgaccactt
     4981 caggcctcga cccaggtttc ctggcactgg gcgggggcgt cccgctcata tctggccgac
     5041 ccatggactg accctggcgt cccatcatcc ccatcatgcc acccatgtcc aatttcgtga
     5101 acccgtcagg aatctcgaag agcttcggat cctgtctgcc gatccggaga ttgctgagtt
     5161 cggtcatcat ccgcttcttg tcgttgcctt ccttgtagag aaggtcctgc ttgacgttga
     5221 tgccctcttt cgtccgccag gaaaagccgc cgaatttttt tccgtcggtg ctagtcgcga
     5281 tggtcttgta cttcgtcacc ttgacgccgt tcagtgtttc ttctcccatt gccgtgtctt
     5341 cgtatgtcca ttgagacgta tcatttcctt tcgagggacc tcctccgatc gaatattcca
     5401 tgtacatgtt ctcggatggc atgagcatcc acatcacctt ggtgtcgtag cggaaaatct
     5461 gaaccgcccc atcgcctgcg cctgtcaaca tttccttgcg ctctttggtc ggcgttacga
     5521 aaatcttttg cgtcgtcgca ccttcttcat tttcaatcgt tgcatcggcc gaatactccg
     5581 cctgcgggcg attcattgga gctgcctgcg caggcagcca ggtcgagagc gtcgcaagag
     5641 cgaccaggac gaggagaatg tgtggcatgg caaactcctt atggaatttt gaggtgctcc
     5701 agtctctgcg aacacactgg tttcagaagt caaaaactga cgtcgaaggg tatcgccgga
     5761 tgggccagaa gtaaaggaaa caattcctaa gaagaagctc tggagtgttg ttgccgcctc
     5821 actttcccga ctgctgaatc agcttcgcga cgagtccggt ccagccggtc tgatggctgg
     5881 cgccgatgcc ggctccgtta tctccgtgaa agtactcata aaacagcagg tggtcctgcc
     5941 agtgtggacc ctgctggaac atttgcgctc cgccgaacac cggccgcttc ccttcggcat
     6001 tacgaagaaa aatgccgatc aaacgccgag agagctctcc ggccacctgc cagagggtgg
     6061 cctgctgtcc cgacccgaac ggatattcca ccttgtagtg atcgcccaga aaaaagtgga
     6121 atttctgtag cgattcgatc aagagatagt tgaccggaaa ccagatcggc ccgcgccagt
     6181 tggaattccc gccgaactga ggagtgctcg attcagccgg ctcgtaatcg actcggtgct
     6241 catgtcccat gacgttgagc acatacggat gatccctatg gtgccgcgag agggcgcgga
     6301 tgccataagg cgaaaggaac tcattctcgt caagcagata ccgcatgatg gagcgcagcc
     6361 gatggcgatt cacaagcgag agaaatcgcc tgacgccgcc ctcgtagctc tgcgtttcca
     6421 cgtgctgggc gaattccggc cgattttcga tgaaccattg catccgatgt ttgaaccgcg
     6481 ggaggcggtc gatgagcgtc gaatcgagcg tttccaccgc gaacaacggt atcagtccca
     6541 ccatggaacg gaccttcatg tgggtcgtct ctccgttcgg cagatgcagg acgtcataaa
     6601 aaaatccgtc ggtaccatcc cacaactcga tgcgtgtacc gccgatgttg ttcacggcgc
     6661 ggcagatgta cacgaaatgc tcgaagaact tgctggccac atcttcgtat gctcggttct
     6721 cacccgcgag ctccagggca atggtcagca tattgaggca gtacatgccc atccagctcg
     6781 tggcgtcgga ttgttcgagt cgccctccgg tgggaagcgg cgcgctccga tcaaagacgc
     6841 cgatattgtc gaggccgaga aacccgccct gaaaaatgtt cttaccttcc gtgtcctttc
     6901 gattgaccca ccaggtgaaa ttcaagagga gtttgtgaaa gacgcgttcg agaaacacgc
     6961 ggtccccgac gcctcggcgt ttttgttcga tcttgtagac ccgccacgcg gcccaggcat
     7021 gcaccggtgg gttcacatcg cccaaggccc attcataggc gggcatctgt ccattgggat
     7081 gcatgtacca ctctcgcagc atcagtatga gctgttcctt ggcgaatgtc ggatcgacca
     7141 gcgcgagcgg aatacaatgg aatgcaaggt cccatgcggc gtaccagggg tactcccact
     7201 tgtccggcat ggacaggacg tcggcgttgt agagatgcgt ccagtcggca ttgcggccat
     7261 ggagtcggtt tggcggaggc tcgggtccca tgggatcgcc cttgagccac cggctgacat
     7321 cgtagtgata gtactgcttg ctcaagagca gtccggcgaa ggcctgtcgc tggacgcggc
     7381 gggcgtcttt cgaaaggcgg cgaggagcca gcgtctcgta gaattcatcg gcttcctgaa
     7441 tccgcctctc gaagacctct tcgaaatttt gtcgcgtgaa gccggcgacg tgagcatcat
     7501 tcgtaaagcg gagattgatc ctgcctgcgg cgccagcggc tacggtgagg gcatagtgcg
     7561 ccgccgcttt actgccgata tgatcggggt tgatagccgc tttcaggccc cagaccacat
     7621 agtcgtgaaa tgcgtctttc gcataccgtg gtccttcatc gtccgcatag aggcgtctcg
     7681 agttcgtctc gttttccgtg aacaacagcg gcggctgctt gtcgcacagc agccgccgca
     7741 ctccatagta gtcgtggacg gtttcgatga cgctcgcggc atcgatcggc acgcctgcct
     7801 ggaaacgtgg acgtctgatg tcggagcccc atgaccaggt attacggtac cagatggtgg
     7861 gcagcacggt gagctctgcg gggtcaggac ctcgattaat cacctctacg cggatgcaga
     7921 tgtcttccgg cgatgccttg gcatactcaa cgaagacgtc gaagtagcgc tgggcatcaa
     7981 agcacccgct ctcgtgcagc tcaaattccg gctcgtggcg gctccggcgc cgattctctt
     8041 caaccagccg ctcataggga aaggtcgatt gtgggtactt gtacagaaac ttcatgtacg
     8101 aatgggtggg tgtggaatcc agataaaagt aatactcctt aacgtcctcg ccgtggttgc
     8161 cttcatggcc ggtgagaccg aagagccgct ctttcaggat tgggtcgtgc ccgttccaga
     8221 gcgcaagcgc gaagcatatg tattggtgac ggtcgcaaag gccggcgaga ccgtcttcgt
     8281 tccaccggta gacgcgtgag cgggcgtgtt catgaggaaa cgcttcccag gccgttccgg
     8341 aaggactgta atcttcacgc acggttcccc acgcccgctc gcttaaatag ggtccccatc
     8401 gtttccaatg ttgttcgcgg cgcgcatctt cttgaagacg tttctcttcc gccgtcactg
     8461 gagggcgtgc cttagcagtg ggctgtcgtc gtgaagccat gtgtgtcctc gagtcgacta
     8521 attagacacc ggctgttttc gaaggagctt caacgacacg gaccgtgtag aggtagtgat
     8581 acagcccctg ccgagccatc aactcgtcat gggttcccgt ttctacgagg cggcctttgt
     8641 ccaaaaccag aatccgatcg gcgctgtgaa tggtggtcaa gcggtgggcg atgacgaagg
     8701 tcgtgcgtcc cctcatcagc cgttcgagcg cttcctggat tagccgttcg gactcgctgt
     8761 ccagtgctga ggtcgcctca tcaagcagga gtatgcgcgg attcttgagt agagctcgag
     8821 caatcgcgat acgctggcgt tgtccaccag agatattgat gcccttttct cccacaacgg
     8881 tgtggtaacc atcgggaaaa cccatgatga actcatgggc attggctgcc cggctggctt
     8941 ccttcacggc ctcttcgtcg gcgtcaccgt tgccataccg aatgttgtcg agaatcgtcc
     9001 ccccaaacag gatggtctcc tgcggcacca gcgcgatttg cctataccaa ctctccaacg
     9061 tgacatcacg gagacttgtt ccatcgatgg tcacgcgccc ttcggtcgga tcataaaacc
     9121 gatggatcaa attcatcagg gtggtcttgc ctgcgccggt cggacccaca attgccacac
     9181 attccccagg tttcgcttcg aacgacacat cgatcaatat cgcccggcgt ggatcatagg
     9241 caaagctcac atgttctgcg gtgagttggc ccgatacggt gtgaagcaca agggcgtttg
     9301 gatcatcccg gacctcgggc ctggtatcca ggatttcaaa cacgcgctcg gtcgcgccct
     9361 gagcttcccg aatttgcgca aacacacgtg cggcggaact gaacggcccg atgagaatac
     9421 cggcaaataa cacgaaggcg aacaactcac caggcgagac ggtgccatcg atcacctgac
     9481 gtcctccgta ccataagacg gctgccgcag aggaaaatgt cagcagactg atgacaggaa
     9541 tgaacaccgc catgatcccg gcccgccgca tggtgagtga gagcgttgtg tcgacctgct
     9601 gcgcgaagcg tgcttcttcc cgctgggtct tgacgaagga cttgacgatg cggatgcctg
     9661 agatgacttc ttcgagaagc gtactcaggg cggcggtctg atcttgaatg gaggtggaaa
     9721 gcgatttaag ccggcgaccg aacagcttgg ccaccacgac gagcaagggc aggaggacga
     9781 gaatcaacag acaaaggcgc caattcatcg cgagcaggaa ggtgatgccg cccacgaatg
     9841 tcacgagctg tttggctgtg tcgattggag tttccgtcac caccgattga atcaccgtga
     9901 cgtcgttcat gagccgtgac agaagctcgc cggtcctccg cctggcaaaa aaactgacgg
     9961 agagcgtctg caggtgagcg aagaggtgcc ggcgaaaatc ggcgacgatc cgttgtgaga
    10021 ccgatgccgt caaataactg tggcccatgg agcagaatcc ctgcaggaac acgaggctga
    10081 ggaagaggag gatgagttcg ttcatcctgg caccatcgtg ctgaacggtg atgacatccc
    10141 acagcgctcc cgccagacgg agtaaggcga gattgatggc ggccacggcc atgaccaaca
    10201 cagtggcaag caccatgcgc cccagatagg gtgcgagaaa gggccgaaat cgtgcgaagg
    10261 tggacataac cgtcagatct tagtgaacgg ccgaaaggaa aaccagtgca gggttcaaag
    10321 cttgcgactc ggataaatca ggagggatga ggaaggatct gacctagagt tgggcgatgg
    10381 actcgatgag attcttgttg atggcaacga aatccgagcg atccttgtcg ttgacgacga
    10441 cgtcggtcag gctaatgaag agccgttgct cctcgttaat cgcatccgag acgcggtttc
    10501 gcatgggggg aatcagaaaa tcacccacat acacgcagtt caccgtacgg acgcgtatgc
    10561 gaaccttctt cccttcgggc accgcccctc ctccgtatgt cggtgagact agcttttacg
    10621 gcgcaagaac ttttgacgcc tgcgcaactt tttgtactta tgtttgcgca tcttcttgcg
    10681 gcgttttttg agcacgcttg ccatacggtg tctcccctac gcgcgtgagt ctagaagttt
    10741 tgggccgaaa ttgcaaggca acgacacgtc gtgtgcatga cgcagaggtt ggcttgaccg
    10801 gtccaacgag ccgttcctat actgcaatca cgatgcattc ctcgagacat caggtaaccg
    10861 cgctcgcgat cgtcctcgtt ctctggatgg tcggcacggg ctgcggctcg aaaacgatgc
    10921 aatatcccga agatcatgag cggtaccttc gtatcgataa agcggtcgaa tcgcttcggg
    10981 tagcgtacgt gaagaaggat agtgccggca tcgcagcgct cgtggtgccg acagagtcca
    11041 cggagcggct gcagcaggag gcagaagccg attttgaatt gttccacgac atggccatgg
    11101 actttcacgt cgagcgcatc atgatcgaca atgacgacat cgatgtctac gtgcattggc
    11161 aagggttgtg gaaaaaagac ccggaggatc ccggtatccg ccatcgcggt cattcgcgcc
    11221 ttcaatgggt gggtacgaaa tctgtgctgt tgagcggcat ccaaggagat atccccttcg
    11281 gagtgaacag acgccaagcc ttgacgggtc aggctcccgg cgttgggcgg aagtagtgat
    11341 gccggccaat cgtttatctt cccttctctc cgcggatttt ctcgtcatcg gcagcggagt
    11401 cgccggtctt cgggcagcgg tcgaactgag ccggaacggc caggtggtgg tcatcacgaa
    11461 ggggcatccg ctccagagca gttcaatcca tgcgcagggt ggcgttgcag tcgcgctgag
    11521 cgaggaagat gacgtcgcga tccatttgac cgatacactc aaggccggcc acgggctgtg
    11581 ccggaaggaa gccgtgcgcg tcttggtcgg ggagggaccg cagcgcatcc aagagttgat
    11641 caaatgggga gccaggttcg acaaagccgg cggcaaattt gcgtttgccc gggaggcggc
    11701 gcacagccgg agcagaattt tgcgcgcccg aggcgatgcc accgggaatg aaatggtccg
    11761 ggtgctgatc gccgaggcga tgcggcagaa gcgcatccaa cggctggact atcactttac
    11821 ggtcgatctg attgtggaga gtgggcgctg ctgcggggcg ctcgtgttcg atgagcattc
    11881 gggtcgacga ttcatacttc cggcgaaagc ggtggtgctg acgacgggtg gggcggggca
    11941 gctctatgcc cggactacca atcctgcgaa tgccaccgga gacggcatcg ccatggcgct
    12001 gcgagccggc gccgtcctgc aggatatgga gttcgtgcag tttcatccca cggcgttgta
    12061 cctaccctcg agccccccct tcctattgtc cgaggctatg cgcggagagg gggctcaact
    12121 ccgaaacaac aaaggcgaaa tcttcatgcc gcggtatcat ccgctcggag ctcttgctcc
    12181 ccgtgacatt gtgtcgcgtg caatctgggc cgagatggcc gccacaaaag cccggcacgt
    12241 ttacttggac gtcacgcatc ttggcgccga attcgtcaag cgtcggtttc cgacgatcta
    12301 tgccacatgc ctgcgttatg acatcgacat taccgaggag tggattcccg tgtctcccag
    12361 cgcccattac atgatgggcg gcgtatggac ggacttgaat ggcgccacga ccgtcccggg
    12421 attgtttgca gcgggcgaag tggcgtgcag cggcgtccat ggggcgaacc gactggcaag
    12481 taattcatta ctcgaaggac tggtctttgg cgcgcgggcg gctactgcgg cggtagccta
    12541 tgcgggccgt gaagaaattc cgtctctggc tacgcacgaa gcggctctca agcacgaaca
    12601 gtatggttcg cttgacgatg tggaaaaatt gcgcagctca cttcggcgca cgatgtgggg
    12661 tcaggtgggg gtgatccgtt ccggcgaatc gctgatccgg gcctgcgcgc agctgtcccg
    12721 ctgggcgcaa ctggttgcac agccgttttc tcagcggacg gcgctggagg tcaaaaacat
    12781 ggtgcaggtt gcgcagtgta ttgcggaagc tgctctatgg agggaaaaca gcgtgggggc
    12841 ccattaccgg tcggactttc ctcaggccaa gcgaccggga tggcaacaac acagtcggtt
    12901 gtcgctgggc gagggaatca gcgggacgcc tccacggaag tcgcgggggc ttctgctggc
    12961 gttgaagggg cgtgccggct gacgggacgc tcggtgatga caccgtcgcg aacgaggaag
    13021 gtccggtaat agcgcaagac tttgcggacg tattgtctcg tttcgtcgat cggcggcaag
    13081 gcttgataac gatcaacgat gttctcgccc gcattgtagg ccgccagggc aagaggcaaa
    13141 tttccgtgga agcggtcgag taactgcctc aagtattttg caccgccccc gacgttctca
    13201 tcaggatcaa acatatcgcg tacgtcgagg cgcacggcgg tctgcggcat gagctgcata
    13261 aggccgatgg ctccggcccg ggaaacggcg cgtgggtcga aattcgactc ggccttgatc
    13321 actgcccgga tcagcgccgg atgcaattgc tgttgagacg aatggcgttt gatgacgggc
    13381 tccagttcac gttccgaaag caatgcgtga aagcgggccg attccacgtc gagcttccga
    13441 tagcgagcgt ctgagggcac gttggtaagc gagatcgtac cgttcggtcc tatatattgg
    13501 tagatttccg cctgtgagcc gacagggttg aggggcgaca gaacgatgac ccccgtcagg
    13561 agcattccgg agctgcatcg gattaagctg gtgatgcgtc gcagtgttgc tgtcatcggc
    13621 atggtcccaa cgatatcagt ctgttccagc ctgtcaagaa aatgcagatc ccatgccgca
    13681 tggcagcgca ggaaacaaga ggcagaacaa gaggatcgga tggagaggct acgcaagctc
    13741 cagaaatttg tcacggtgag tgacaaaaat ttggtgaagc cgtttgctca gtgggccggg
    13801 cgttccgttc ccgatcggcc gaccattcag ctgcgtggcc ggcatgactt ccatcgtcgt
    13861 gttgctgaga aagcactcct cggccgtctg aagcgactcc acggtaaatt ggccttcttc
    13921 gacaggaatc gctgattcac gcgccaaccc cagaaggaca cctcgtgtaa tgccgtccaa
    13981 aatgccacag gaaagagccg gggtgcagag ccggccggat cgcacgaaga acagattgct
    14041 gaccgtacac tcggtgaggt gagattccca attgagcaac acgctgtcaa aggccccggc
    14101 gttgagcgct tctcgtttcg cctggatgtt gttcaggaaa ttcgtggcct tgatttgtgg
    14161 gtcgagcgcg cttggaagat ttcgcctggt cttggccacg atcaggctca cgccggtccg
    14221 atagcaatcc tgtggcagcg gtttgagcgg tttggccatg atcacgatcg tcggagtggg
    14281 gcagagcgcc ggatcaagac cgatctcgcc gaccccccgt gagatcgtca cgcgaatata
    14341 agcgtccgtg tgttcatcgc ccacgccgtt ccgggccatc gcctcatgga gaactgacgg
    14401 ccagttcagt tgcggaatgg acaggccaat gccgtctgcc gagcgtcgca agcgagccag
    14461 gtgctggtcg cgcatgaaaa tcttgtgacc gtacgagcgg atcgtttcat agacgccgtc
    14521 accgtagaga aagccatggt cgaatactga gacgagcgct tcctcctcct tgacgaaacg
    14581 gtcatgtaag taaatccacc ccatcagcgg cgtccgaccg cgctgaaaaa tgcctgcgcc
    14641 ttctgcaggg tctcttgata ttccttggcg ggatccgagt cggcgacgat accggccccc
    14701 acttggagat atccctgctt ttcggtcatc acaagcgtgc ggataatgat gtttaaatcc
    14761 aaatccccgt tccatccgat atagccgaat gatcctgtat agggtccgcg gcgaaagggc
    14821 tcgagttcct caatgatctc catacaacga attttcggga cgccagtgat ggtcccgccc
    14881 ggaaacgtgg cgctgatgag atcgaaaggc gtcgccccgg gacgcagcgt gccctgtacg
    14941 ttcgagacga tatggctgac atgtgaatac tgctccacca ccatgaattc attgacctgc
    15001 acgctgccga tctcgcaaac acggccgagg tcattccgtt cgagatcgac caacatgacg
    15061 tgctcagccc gttccttttc attggcgagc aactcccgga cgagacggcg gtcgtcatcg
    15121 gaattacagc cacgaggtct ggtgccggcg atgggtctgg tgtcagcacg gcgaccctgg
    15181 aggcgaacca agcgctccgg cgagcaactg atcaacgaca tatcgccgaa ccgcaggagg
    15241 ccggaaaagg gagagggatt caccgagcgg agttcttgat acagcgcctg ttcgtactgt
    15301 aaacgggcag cccccgatcg atagccttgt gggatctcga tgccgaaccg atgggaaagg
    15361 ttggcctggt agatatctcc ggcggcaatg tactcttggc agcgccgcac tcgatcgaga
    15421 taggcctctt gcgtctgttg cggaccaaat gagacggtgg tcagcgccaa cggactaggc
    15481 ggtgtcgcca cggagccggt gagtctggct tcccattccg caaggcggtc gagtccctca
    15541 cgatagagtt tgtcgcgcgc ttcaccgaga aatcgatcga gcgatggaca aaagataact
    15601 tggattcgat tggttcgatg atcgaccgcg gcgaccacct cgtaaagtgc gaagtgcatg
    15661 tcggggagtg agagatcgtc aacggcgact gccggcacgg actccagccg gcgtgcgaaa
    15721 tcataactga agcatccgac tgctccaccg aaaaacggtg gcaaatcggg cgttcgttcg
    15781 atgccgggcg aagcgatgat gtgccgaagc catgaaaaag gatcgtccga tgatgatgcg
    15841 gtcgcaaggg agggtcgtgg catgacagaa gccgggtggc tgccaaagaa tgaatagttt
    15901 ctgttctcgc ctttcccgct ttccaagaga aaggagggct gtgtggagga ggcgaccttt
    15961 tgatacaagt cgaatggact ctcaccgcga cccttgaatg ataggacaag cggccgtggg
    16021 gcaccttgga ggaaggaagg ctgcgaaaat cgggtcatga tcgggtcatg gttggagtcg
    16081 ggttcacgca ggagcagttc actatacccc acggattcgg tgatttccca gcagccggag
    16141 cttgacaagt tcacgagtac tctgcttgaa ttgcgggccc gccattcggc agagtcctga
    16201 ttcaggacac tcgcctcagt gggtcaccga ccatgcccca gtcacaggat ccgttttatg
    16261 cggggctcgg ccaggccgtt cggataggaa cggaactgct ggctgcgctg attgtagggg
    16321 gcggattggg ctgggccgtc gatacctatc tcttggagac cggtccctgg ggtctcgttc
    16381 tcggcttgat cttgggtgcg gcggccggcg tgaggaatgc ttatcgaatg gcgcaacggt
    16441 ggccccagta accgattgac gcatcgcgaa agggtgcatg gaagaaagtc ctctccatcc
    16501 gtttgagctg cacaactata ttccgatcaa tcttggcggc ctggatattt ccatcaacaa
    16561 agccgtcatc atgatgtggg tggtggtggc cctcatttcc atcctcatgg tcacggcggg
    16621 gtctgcccgc cggctcgtgc ccggcaaact gcaaagcctt gccgaaatgt tggtcgattt
    16681 tattcgcggc ataattctgg acacgatggg aaaagacggc atgaagttct ttcctttgat
    16741 tgccacattg tttcttttca ttttgttttg caacctcatt ggattgattc ctggttccta
    16801 tacggtgacc agccagattg tcgtgacggc agtgtttgcc tgcacggtct acggacttag
    16861 cctcctgatg ggcttcgtgt tgcatggcgg gaaattcttt gggatcctcg tgccaccagg
    16921 taccccgggc tggctgctgc ctttgatggt cccgatcgaa ctgatcagcc agttggctcg
    16981 accgatttcg cttgccgttc ggttgtttgc caatatgacg gcaggacacg tgatcctggg
    17041 tgtgttgttc ggattagccg tcagtggagg ggtgttgatt ggatggttgc ccttcgcgtt
    17101 cacgatcgct atgaacggtc tcgaagtcgg catcgcattc atccaagcct acatattcac
    17161 ggtgttgacc tgcgtgtatt tgggcgatgc gtttcatttg catggccacg acgagcatgc
    17221 acactaacgc ggggtgacgc gccaagggcg cggagcgacg cagtctcagt ttgcaacgga
    17281 cgtatttagc aagggaggag tacgagacga tggattcagc agccgcagca cttttgggga
    17341 tgggacttgc cgccgccggg tttgcagggg cgggcgttgg cataggctat atctttggaa
    17401 aaatgatcga ggcagtcgca cgacagccgg aagcggaagg ccgggtcggc aagtatatgt
    17461 ggatcggatt cgcgctggtg gaagccatcg cgctatacgg gttggtcata gccttcatca
    17521 tcatgggcct ccgcaaataa gagatgctga ggaaacagct tccgacgtcg ttcgctggca
    17581 ggtccggtcc tcgttgacgg gtcggagtga cctcttggtt cgcccgacac tacagcgccg
    17641 ttggaaagct gttttgactc ttactgaatc tttagcaaag tgagcgtgag atatgccgca
    17701 atttgaatcg cattttttct cttccctgat tttttgggaa gtggtgtcgt tcgcgattct
    17761 cttgttcgtc ctctataagt ttgcctttcc cggtatttta agcgttcttg aggagcgaga
    17821 gaagaaaatc aagaacagcc tggacgaagc ggaagctcat cgcgtggaag cggagcgcag
    17881 gctaaaagaa tacgaaacga ggctcgcggg ggcgtcgagg gaagccgaag gaattctggc
    17941 ggcggcgaag gagcggacgc agcggcttat ggaagaaaat gagcagcggt tgacggccga
    18001 ggcggagcgg atcaaaggcg atgcgactcg cgaaatcgag catgaacgcc gaaaggcgat
    18061 tcaggaaatc cgcacgcaga cgaccgacct tgccctcatg gtcgccgaaa aagtggtcca
    18121 gcggagtctg accgacgcgg atcatcgacg cttggccgac gaagcgctcg acgcactttc
    18181 aaggtcgtcg cactaatcag gcagacacaa aaaatcctcc ggcgcgcgtt ccgcagaggc
    18241 agaaagtggt gtgaccatct tcttggcgct aggccaagct gatcccgccg ggtcgatatc
    18301 cttccaaatc cacacaaacg aagcgaaagc cgatctctcg caggcgggta ctgactgtta
    18361 agcgtcggcc gggctgattg agtcgtgcga actcctcggg cgccatttcg attcgagcaa
    18421 tgtcgccatg gtccctgacc cgaaagtggc tgaatccttc ttcctgcaag acggcttcgg
    18481 ccgcctcgac tcgtcgcagt ttctccatcg tgatggggat gcctcgtggg atgcgcgacg
    18541 acagacaggc cgcggccggc ttgtcccagc ttgatagacc aagttgatgc gccgccgcac
    18601 ggacgtccgc tttggtcagg gcggcttcaa cgagcgggct ccgcacgttc cattcccggg
    18661 ctgccttaat tcccgggcga tcgtccccca gatcgtcgag gttggttccg tcgagcaccc
    18721 actgcatgga tcgggcgatc cgcagtcgct ccatcagttg atataaatcg gtcttgcaat
    18781 gaaagcagcg tgcgccatcg ttttgtacga aggccggaat cgtcaactga tccgtctgga
    18841 cgatggcatg ctcggcgcca atgtcttgcg cggtacgcgt ggcggcgtcc aactcggcgg
    18901 ctggataggt cggcgagacg gccgtgactc cgactgcgcg tgctcccagt tcgtcatgcg
    18961 cgattttcag taccagggtg ctgtcgatgc cgccggaaaa ggcgatgagg gcggactgca
    19021 tgtcggcgac aaggtggcgg aggcgttcaa ctttatcggt taaggcgtcc atggtcatgg
    19081 agcgttcctt cactgcttca gcttggcctt tgccacgttg ccgaccacca cgagcgcata
    19141 gcgttggggg tagaggtact gcttcgccac ccgttggacg tcttccttcg tcacgcgctc
    19201 gatccattta ggatattgcg agaaatactc aaaacccagg ccgtaaaatt ctacttgagc
    19261 caatacttgg gcgagctttg ccgtggagtc aagtcgcagt ggaaagctgc cgatcaaaaa
    19321 ggatttcgcc tcggagagct cctgatcggt taccggggct tcgcggatac tcttcaattc
    19381 agcaagcacc ccctggatcg cctggtttgt ggcttcagtg cgcgtttgaa aattgaccca
    19441 aaaggagccg ggcatcagcc gcgcatcgaa atgactcatg atgccgtaca ccagcccttg
    19501 cttgtcccgt atggaatcca tcaaccgaga ggagaacccg ccggcgccca ggatatagtt
    19561 catcaccgtt acggcataaa aatcgggatt cgtccggctg atcccgccat ggccgagaat
    19621 gacgttcgat tgagtcaggt ccttttcaat cagctgcacg gtctttttct cgatcggcgg
    19681 agcgctcttc gcgcttcgtg ggggctgtga ggcctttttc caccctccga aatgcgtctg
    19741 cacgaatgtc gtgacctgtt ccgccgtcac gtcgcccacg atcgtgagaa tcatctgact
    19801 tggagcgtat tcacgcgcgt agaatcccac gacgtcggca tgggtaatct tgttcagcga
    19861 ctcctcggtt ccgttgagtg gccagcgata ggggtggccg tgaaagacca gttgattgaa
    19921 ggctttcatt gcgacgtggc caggatcgtc attgtcgctc gccatttcgc ccaaaatctg
    19981 tgaacggatg cgttcaaact caggcttgag aaatgccggg tgttgtaaaa tatcggccag
    20041 taatgtgaat cccagctcca catctttttt cagcactcgg acggaggcgg tcgtaaagtc
    20101 ttcagcggcc ttgacctcca acgcaccgcc gacaaagtcg atctgctccg caagctgctt
    20161 ggaggtgcgg ctggtcgtgc cttcatcaag caaactggcc accagattgg ccaagcccgc
    20221 tttctcggga gggtcctgcg ccgatccgct tttcaccaat gcatgaactt caacgatggg
    20281 caggaaatgc tgttccaata ccacgacagt caagccattc ggtgcgacga attttgtggg
    20341 aacgatttcg gcagcgccgg acggtgagac cgggaacgtc gcgaagacga gagtgaatac
    20401 cgatgtccca aggcaccatt gcagcccacg gcgaagcatc gggtgcaggc gtgtcctgat
    20461 cacggctttc cctgctggac tgaggcagtc ggcggctcgg ccggtttggg tgtgactgga
    20521 atcaagctgc ccaccgtccg ggagtcctcc agcagatact gtccggcgac acgctgcaca
    20581 tccttggctg tgaccgctcg gatccgttct agaaactgat ccaccttgcg ccagcctgct
    20641 cctacggact ctgcctgacc gagcaacatg gcatgccgga agttggagtc ctgctcaaag
    20701 atcctggccg cttccacttg gttcttcgcg cgttgcaatt cctgctcagt cggagcctca
    20761 gactgcagcc gtttgatctc cttgtgcagc gcctcctcga cgacctccac cttttgtcct
    20821 ggactcacga gcgcgtaaaa atagaagaga ccgggatccg tctgcatcaa cccgtactct
    20881 gccccgacgg ccagcgaaag cttctggtcg tacaccaagc tctgatacag ccgcgaactc
    20941 tttccgtgcg agagaattga ttccaggacg tccaacgcat aggagtcctc gctggtgtag
    21001 ttggggacgc gataccccat cattacaaac ggcacttgtg cttcacgctt gaggagaaag
    21061 cggcgctcgc ctttctgctc ggcttccatg gtcgcaaccg gtttgggctc agggccacgt
    21121 gggatgggct cgaataggtg tttgatcgtt ggcagcaatt gctcggcttt gatgtcgccg
    21181 acgacaatca gcgtggcatt gtttggagag tagtatgtgt catagtgccg ctgcaaatcg
    21241 tcgagcgtca tggcgtccaa gtcgccgaac cagccgatca cgggccagtg gtagggatgg
    21301 ctgagatagg cctgcgcaaa cagcgcctcg accagtgcgc cctgcggatc gtcttctgtc
    21361 ctcaggcgac gctcttcctt gacgacttcg cgctcggttt tgagctcatt ctgatccaaa
    21421 atgagccctt gcattctgtc ggcttccatt tcgagggcga gctgcacgcg atcggcagcg
    21481 agattctcaa aataggccgt aaaatcctga ctggtgaatg cgttgtccat gcccccgttt
    21541 ttgcggatta gacgcgagaa tgaacctttc ggatatttcg ctgtgccttt gaacatcatg
    21601 tgttcgagca tgtgcgagag gccggctcgc cccatcactt cattgcgcga cccgaccttg
    21661 taccaaacct gaaccgtcgc gacgggagct ttgggcattt cgacgagcag cactttcatg
    21721 ccgtttgaaa ggaggtactc atgcggttct gcggcggcgg cttgaacgga aactgcgagg
    21781 gcgctgatgg tgagcaacca aaagggaatg tgcgggaagc gcagcctgat catggtcggt
    21841 ggtgagaaac ggaacaatgc atgctaacaa tgagattcaa tttgtgtcaa ggttcgttcg
    21901 tacgacggag ggagatttcc cagttgaccg catgcaccca ggacatcccg gccccggctt
    21961 ttccgaatga acacgtccat gccggcctgt cgcacaattg attggaacgt gaggatgtgt
    22021 cggtcggagg gacgacgaaa cggactgccg ggaaatggat tgaatggaat gaggttgacc
    22081 ttgcagttga tgcccttgat caaggtgacc agccgcttcg catcggcttc tccgtcatta
    22141 atgcccgcca gcagcacgta ctcgaaggtc aaccgtcggt ggcgaggcaa tggataacgg
    22201 cggcaggcag agagcagcgt tttgagtgag gcgataccat gaaccgccgg catcagccga
    22261 gcgcgctgct cttcggtcgt ggcgttcaac gaaaccgcca gattcacacc aagcgcggcc
    22321 acttggtgta atttagaggc gaatcccgcc gtcgagacgg tgatccgccg cggcgaccag
    22381 ccgagtcccc atttcggatt cgtcagccgg cacaccgcct cggacagcgc cgcgagattt
    22441 gccaacggtt cacccattcc catgaacacc atgttcgaaa ggcgttcccc gggatccaag
    22501 tgatcgtgag ccgtcaaaac ttgttcaatg atctcatgcg ccctcagatt gcgcttcagc
    22561 cccattgtgc ccgtcaggca gaagccgcag tccaaggtgc agccaacctg cgtagacaca
    22621 cagagcgtca gccgctcgtc gtcggggatc agaacagctt ccacctcgag ttcgtcatcc
    22681 agcttcaaga tcagttttct ggtgccatct gctgatcgga acaccgtgca tccaagcgat
    22741 cgtccgatcc tcgcttcgcg ttccagcgtc gcccggtccg cttgactgag gtcggtcatt
    22801 tgactgatcg tccgtgcccg ccgttggtag agccagcgaa gaatctggcc ggtgcgatag
    22861 gcaggccagc ccaggcgctc aacgagcgca gctgtctcgg actcgtcgag agcaagcaga
    22921 ttcatcgtcg tcggtggggt catcgtcttc acgccacgca tggcgcagcc taacacacgc
    22981 agaaaaagtt gaacaagcaa tccgcaggat gtcttctgct ggcatggatc gaaggacact
    23041 gttatatatg gtagggtcat caggcttggt gatgaggttg gtcgtggttg ctgttttggt
    23101 caggtgctgt gcgtgcgccc tcctgctcct gattaccgga gcggccggct cggctgctct
    23161 tccaggtgaa tcggacccct gtgagcagcc tgagctgtgt tttcaggcag cggcattccc
    23221 caaagaacgg ctcgggaaag tcatgaacaa ggaacaggtt ctcacgctca agctcgagcg
    23281 gcttcaacag ttggcggatc ggttttctgg caccctgtgg gggaaacggg cggggcttct
    23341 gtcgggcgtg ttgttgatcg agcgtgaccc cgcttcagcc atgcaatttc ttcgcggtgc
    23401 gcagcgggat tttcctgtcc tggatgacta catccgcgtg tggatcgctg aatcccagtt
    23461 gaatctggga caagccaaag aggcggcggt gttgttggaa agcattccaa tggcagtgcc
    23521 cgattccaac atactgggca aggcggccta taagacgggt gaggcctggt atcaggcggc
    23581 cgcttgcgcc gaatcgaccg cctggttcgg cagagccgtc gcgatgaacg acaaggatgc
    23641 cagcgcaccc caggcgtacc tgcgacgggg cgcgtgccgc ctgcgggaca acaatgtcgt
    23701 cgaagggcgc gagctgctgc agcaggtatg ggttcggttt ccctacaccc gtgaagccaa
    23761 ggaagcggaa accttattga cgtcgaatct tggcggtgac acgtggaccg tgcagcccgt
    23821 cgatcggctg aacagagccc aggccttctt gggacaggcg tttcacgccg aggcgatcga
    23881 agagctgaaa aaatttctct tggccgatcc tcactcgcct cgacgagccg aggccaagtt
    23941 gaagttggga atcgcacaag tgcgactcaa gctctacgat cacgcgcgtg aaacgttccg
    24001 cggcctggca aaagagggag gaccagagtc gcgcgaggca gcggtgtggc tggcgcgcgt
    24061 ctatttgcgg cagggacaag gcgataagct tttggaagcg gcccgggcca tatcctcatc
    24121 ctctctctcc gccgaacaaa agggccagat cacgttattt gccggcatgt ggctggaaga
    24181 tcaaaagcag ttcgacgacg cgattgctaa atatgtgcat gttgcacacc atggcgaacc
    24241 caccgcccag cgggccgagg gccaatggcg cgtcgggtgg gtctattatc gcaccgctcg
    24301 atatcgcgag gcgggggaga gcttacgggc gctcgccgag caacatgaca gtgaattcgc
    24361 accccaagcg ctctattgga taggacgagc cggtgaacta aacaatcagc cactggaagc
    24421 gcaggacttc taccgcaagc tctgtcagcg gcacccgttc acgtactatt gtcaattggc
    24481 gagagaacgg ctgaacatcc ctcccattgc tgatgccccc atcgaaacgc tggtgctttc
    24541 ttccgacacc gccaagaccg gtgatccggc ccggcccccg gtgacacaag ccgacatcga
    24601 acagcacacg gcctaccgtc gggcgattga actcaagacg ttgggactgg atgcggatgc
    24661 cgctcatgaa gtcgccgtct tgacggatcg atatagccgc gatccggatg tcttgatcgc
    24721 actctctacc atgttgaacg aagtcggtgc ctatcaccag gccctgcgac tcgcccgcgc
    24781 ccgattccgc gatcaattgg agcggactgg cggggcgttt gctccctcac tctggaatgt
    24841 ggcctatccg accggcctgc tgcccaccat taaattacag gccgcaaagg gcgtcgaccc
    24901 ctatctgatt gccgccatca tccgggaaga aagccaatat gatgtgaagg cggtttcgcg
    24961 agtgggcgcc atcggcttaa tgcaagtcat gcccgccacg gcgaataacg tcgcccagcg
    25021 agtggggctt cccgccgtcg ggcgtgaaga cctgttcgat caggaaacca atattcgtat
    25081 cggcgttcgc tacgtcgagc aattgctgga acagttttcc ggaaacctcg tctacaccgt
    25141 ggcctcgtat aatgcgggtc cattggcggt cggcaactgg attgcgcaat accgcggtca
    25201 caaccaggac gagttcgtcg agctgattcc gtatcaggaa accagacagt atgtaaaacg
    25261 ggtcttgcgc agttaccggg agtacgtacg gctcaacggg ctatcctgaa ggcgttgaca
    25321 agccctgggt aaattcctat agtgcgccac aatcggtagt ggcttcaaag taggtacagg
    25381 cacatggcat tggatcgttt ggatgcgctt gaaacacgca tcagagactt ggtcaagttg
    25441 atacaagagc tgaaaaagcg aaatgctggc ctcgaggacg atttgcgatc gactcggcag
    25501 cgcttagcgg acgaaagtga ttccaatcgc cggtgggccc gagaacgttc ggacatcaaa
    25561 gcccgtgtcg aaaaagtgct cggagatgtc gagctgttgg aggcatttga agaacgcaag
    25621 gaggtggcct ttgactaaga cgacggatgt cgaaatctac ggccagcgtt attccatcaa
    25681 gggagaggcg gacgagacct atatccgccg gctggcaggg ttcgtggacg ggcaaatgcg
    25741 acagctcgca gaaggcatga atacgacgac tccgtcgagg cttgcggtcc ttacggctct
    25801 caatctggcc catcaactgt tggaagctga aaagaaacgc gcccaggggg aagcggatgt
    25861 cgaacgccgg atgacgtccc tcatggagtc catcgaagag caggtgccca cctcgctgtt
    25921 tcgatgaatg cgcttgcaaa gcccggaagg cctttgctat cgtggtgccg tggtagtggt
    25981 atttgcggaa gcaggtttat ttattgggat gaggacgagg taaccgccaa ccggtaagaa
    26041 aatccgggat ccgattgcgt cactggaaag aatggatgtg ggtttgtcga tgctggtccc
    26101 gttgagagag tggagcgttt tccaagggtt cgtgcttctt ctatcagccc tgcctgccat
    26161 attggacagg ctagtggagc acgtgctgcc gactgaaccg gctttgggcc ggagtaggcc
    26221 ggcactggtt tccatttcag tatacccccg cctggtgtgc ttgaggttca caggagtgag
    26281 ccgttacccc agagcggatg ggagtgtgtc cggatggaaa caggcgcgtt ccgttctgcc
    26341 aacaccacgt tcatccttgc ggagtcgtga ctcggggtag ccggtccggt ctagcgtcta
    26401 tcgtttgact cgtctctttc ctgccttccc tatacccttc tcgaccatag cttgtgtgag
    26461 gcgagactgc ctggcgcctc aatcgtgaaa ggggccgtcc tatgtccacc tcgattgtga
    26521 tgtacatact cgtcggattg gtaggagcgt tcctcggggc gggtctgttt gaactggtgc
    26581 gccgttcatc ggtgaccgca aaacgggctc aggaagcaga ccaggcgcgt caaacgattc
    26641 aaaacgcgca acgcgaagct gaaaacatcg tcaaggaggc caaactcgaa gccaaggatc
    26701 tggtgtttca gtcaaaagcg gaattggaga aggagcaaaa ggccaagctg ctggaactgt
    26761 ccatgacaga gaagcgcctg gtgcaacgtg aagaagccat tgataagcgc atcagcgccc
    26821 tagacaagcg agacgcagac ggccaaaaac gggaccagga tttgatcagg aaagaagaga
    26881 agctgatcgc aaaggaagcc gcctgcgccc aggccgaaag agagcatcgc gaggcgcttg
    26941 agcgggtggc cggtatgacc gccgacgagg ccaagagaca tctgttgcag gaaatggaaa
    27001 gtcaagcgcg tctcgatgcg gccggcattg ccaaacggac actggaagaa gccagggaga
    27061 atgccgaaag ggaagcacgc gaaatcatca cgacttcgat tcagcgcgtg gtgcgggact
    27121 atgtctctga gtccacgatt tcagtcgtgc ccatccccaa cgacgccatg aagggacgca
    27181 tcattggccg cgaaggccgc aacattcgag ccatcgaggc ggcgaccggc atcgatctga
    27241 ttatcgatga gacgcccgag gcggttatca tttcaggatt cgatcccttg cgacgggaaa
    27301 tagccaaggt ctcgcttgaa cggttgatgc atgatggacg catccatccg acgcggatcg
    27361 aagagatcgt cgaaaaggtg aaagtcgata ttgacaagct gatgtatgaa gaagccgaga
    27421 aaatcatctt cgaattgggc ctgtcggatt ttcatcccga gctgatcaaa gtcctgggcc
    27481 ggttgaaata ccggaccagt tacggacaaa acaatctgta ccatgctcgt gaagcttcct
    27541 atatttgtgg gatcatggcc tcagagctcg gtttggacgt acggctggcc aggcgaggtg
    27601 cgctcttgca tgacatcggg aaagccgtaa gtcacgagga agaggggccc catgccatgc
    27661 tcggggcgga gatagcaaag aaatatggcg agagtgagaa gatcgtcaat gccattgccg
    27721 cgcaccacga acaggtcgag ccgatctgtc cggaatcagt gttggtggct gccgctgagg
    27781 cgttgtcggc cgcccgcccg ggtgccaggc gtgaggcgct cgaatcttac gtcaagcggc
    27841 tggaaaaatt ggaatcgctt gctcacgccc agaagggcgt tcaaaaggcc tacgccattc
    27901 aggcaggaag agaaatccgg gtgattgtcc ggcaggagga cgtgacggat agtgagtcct
    27961 ttcagctttc aagggaattg gccaagaaaa tcgaacagga gttgacttat cccggtcaga
    28021 tcaaagtgac cgtcatccgc gagagtcggt acatcgagta cgccaaatga aggttttgta
    28081 cattggcgac atcatgggag agcctgggcg gcgtgcggtt gcacgggccg ttccgcgctt
    28141 ggtatctcag cgtcagattg atgtggtcgt tggtaacggc gaaaacgtcg ccggcgggtt
    28201 cggaatcacg ccagacctgg ccgatgaact gttcgggatc ggactgtccg cgatcacgac
    28261 cggcaatcac gcctgggata agaaggagat cctcgattat ttcccgcgcg aacggcggct
    28321 gctccgaccg gccaactatc ccgaaggcgt gccggggcag ggcagcgtcg tcattcgaac
    28381 caagagtggt gaagaagtcg ctgtcttaca gttgatgggg cgcgcgtata tgcccacgct
    28441 cgattgcccg ttccaggtcg caaagcgcga gatgtcgcga ttgaggaaac tggtcgctgc
    28501 agtgatcgtg gacatgcatg cggaagccac ctcggaaaaa atggcgatgg gtcattatct
    28561 cgacggggag gcgactgcgg tggtcggaac ccacacccac gtgcaaacgg cagacgagca
    28621 gattcttccc agaggcaccg cgtatctcac cgacatcggc atgacagggc ctctgcatgg
    28681 cgtgattggt gtgaagaaag agcttgccat cgaaaaattt ctgacgggca tgccccggcg
    28741 cttcgaggtt gcgggaggcc ccacggtatt ttgcgctgcg ttgatcgagt tggacgctcg
    28801 catcggcaag gcgctgtcaa tcgaacggat ccggttcact gactaggatg tcggccacaa
    28861 ggcaacagac attatttcac ccctcaaagt cggtgctgac cgtttcccaa ctcaccggct
    28921 tactccgcac caccgtcgaa ggccagttct cggacgtctg gctcgaagga gaagtgtcga
    28981 accttcgcat gccgggttct ggtcacatct attgcacgct caaggacgaa tccagccaga
    29041 tccgagcggt gctgtttcga tccagtgcct tacgcctcaa gtttacgctt caagaaggca
    29101 tgtgcatcat cgtccggggt aggctgaccg tctacgagcc acggggtgag tatcagatcg
    29161 tcctggaaac ggttgagccg aaaggcatcg gcgccttgca gttggcgttt gagcaactca
    29221 aggcgcgact cgctgctgaa ggcctgttcg atgaagccag gaaaaaacca ctgccgacat
    29281 ttccgtccac cattggggtc gtgacttcac cgaccggggc ggccattcgg gacatgctct
    29341 cggtcctcca tcggcggtgg ccgattctcc atatcattct ggttccggtg cctgtccagg
    29401 gggaaggcgc tggtcaacac gtcgcggatg cggtggcatt gttgaatgaa caggctgtgg
    29461 tggatgtgat tattgtgggt cggggtggag gttccatgga ggatctctgg agtttcaacg
    29521 aagaggtcgt tgtccgtgcc gtggctgcct ctcgtatccc gatcgtctcc gcggtcggac
    29581 acgagacgga tgtgacgctg accgacttcg ccgccgaccg ccgggcggcg accccttctg
    29641 cagctgcaga gactgtcgtg cccgttttgg ctgacgtcgt tgagcggttg cgggttttga
    29701 cggtgcgcaa cggccatgcg atggccaggt attgtgtgct ggagcagagg cggttggatg
    29761 cctacatcag cggccttgcg caagtccagc tgcgcatcca gcgcgagtgg cagcggaccg
    29821 acggaaccat cggccgtttg aaaacgcttg tatgcgagag ggtggcggtg agtcgggatc
    29881 tggtccgtca acatcaacga gaactagccg gcctcaatcc gatattttat gtgaagcgcg
    29941 gcctctcgct cattccaaca ttggtccacc gtctggaacg ccaaatcctc gtattgagcg
    30001 aagcacgaaa acgacggatc gctgcgatgg cggcgcagtt aacacagctg agcccgctgg
    30061 caattttggg gcgcggatac agcattctca tgcgcgcccg cgatggcacg attcttcgca
    30121 gcgcgctgga tgcggaagtg gacgaagaac tcgtggcgcg cttggcacag ggtgagctcg
    30181 actgtacggt caagcgcgtg gtgcccgatc cactggtgta aatccactgg tgggctcggt
    30241 ataatgtgcc ttcacaaggt gagaggacgg tatcattgtg ggcggagtga agttcgaaca
    30301 ggcgatggcg cgattggaag ccattgtcgg cgaattggaa aaaggggatc ttcccctgga
    30361 tgaatccctc aagatttttg aggagggcat tcgtctctcc aagagctgtc tgaagatctt
    30421 ggaggatgcc gaacgcaaag tggaagtgct cgtccaggat agaaacggca agaaaagact
    30481 tcatgcgttc tcaccggacg aagaagccga agaatccgcc gacgagtcgt cttgttgagt
    30541 tcgaggtgcg cccgttcagt cgtgtgcctt cctagttcag cttcgattcc cttttcacat
    30601 ctcatgagct cgaaagcgcc cgttgcaaaa gagcgattgg atcgtctgct ggtcagccag
    30661 ggcctttcaa cgagccgtga gatcgctgtc aggaccattc tcgctggagg ggtgcgggtc
    30721 gatggcacgg tggtcgataa gcccgcgcgg ctggtgccgc gtggcgcgcg cattgaaatc
    30781 gaacgacctg ccccttttgt cagccggtcc ggggagaaac tggcggcggc tctcgatgcc
    30841 ttttcgatcg actcacgggg acgagtcgga ctcgacgtcg gttgctccac cggtggattt
    30901 acggattgtc tcctccaacg cgggataaaa cgagtctacg cggtggatgt cgggtatggg
    30961 cttatcgact ggaagttgcg tcaggactcc cgcgtcattc tgctggagcg aacgaatatc
    31021 cggtacgtgg accggtcgct cataccagaa ccaatcgatc ttgccgtcat cgacgtatcc
    31081 ttcatctcgc tgaagctggt gttgccgagt gtcgtgcctt tgttgcgtga agatgctcat
    31141 gtcatcgcac tcgtcaagcc gcaatttgag gtcggcaaag gccaggttgg agctggcggc
    31201 attgtacgag atgacgcgca gcgccgcgcc gtgacggagc gggtgattga atgcgccgct
    31261 gggcttggac ttcaccatac cggtacattg gattctccgg tgaaaggccg gaaagggaat
    31321 cgggaaattc ttgtcagctt tctgcgtcgg ggtataatcg cgtcttcata agcggtgatc
    31381 gcgaaggacg ggaggaagca tgaaggtgtt agtaacgggc ggtgcggggt tcattgggtc
    31441 gcatctggtc gaccggcttg tcatggaagg acatgaggcg gtcgttgtcg acaatctgat
    31501 aacgggaaaa cgcagaaacg tcaatcgtgc agcccgttta tataagatgg acattcaaag
    31561 ctggcggctt gagcgggtgt ttcgaaacga acggccgaac gtcgtcatgc acttggcggc
    31621 acaaatggat gtgcgcaagt cggtggaaga ccctatgttc gacgcgcagg tcaatattct
    31681 cggcacgttg aatgttctcc agcaagcggt caaacacggt gtacggaaag tcattttttc
    31741 gtcctcggga ggagcgatct acggtgaaca ggaaacgtat cctgctcccg agactcacgt
    31801 gctaaagccg ttgtctcctt acggcttgag caaactgtgc ggcgagcagt atctctcgta
    31861 cttccagcgc gtcagtgggc ttcaagccgt cagccttcgg tatgccaatg tgtatgggcc
    31921 gcgtcaggat ccggagggag aagcaggagt cgtggccata ttcatacaga agatgctgaa
    31981 caatgagcaa ccggtcatca atggaaacgg acgacaaacg cgcgattttg tcttcgtcga
    32041 tgacgtcgtt gaagccaatt tggccatgat cggtcaggaa acgcagggca catataacgt
    32101 cggtacgggc gtcgaaacgt ccatcaatga tctttttagg atcttagtcc agcatacggg
    32161 ctcggtttgc aaggagattc acggccctgc caagaagggt gagcaggccc gcagtgtcat
    32221 cgacaacaca aaactgcgtc aggaactttc ctgggagccg agagccgatc tggtcgatgg
    32281 cctgaagaag acggtcgagt atttccaggc gcgcgtacat tgacaacggc gcagctctgg
    32341 catcacgcag gcgggcgggc actaatatct cgggactgat cggtccactt gcgctgacca
    32401 agcatcgatc ccacccacca ggttcttcac attggagaat ccttgttgga ggaggaaatt
    32461 ggtcgcgtcg gcacttctca ttccatgatg gc
//
LOCUS       DASSCQ010000075        40519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1551851, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000075 DASSCQ010000000
VERSION     DASSCQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 40519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40519
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551851"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFH05_05040"
     CDS             complement(<1..212)
                     /locus_tag="VFH05_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677609.1"
                     /translation="MIRVVLPAHLRTLARVAGEVTVNVCGPVTQRSVLDAVEDGYPML
                     KGTIRDHVTKRRRAFVRFFACEQDLS"
     gene            complement(301..1476)
                     /locus_tag="VFH05_05045"
     CDS             complement(301..1476)
                     /locus_tag="VFH05_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET6677610.1"
                     /translation="MSRVRVLVGTKKGAFILTADGKRKQWDINGPHFAGWEMYHLKGS
                     PADPNRLYASQSSSWFGQIIQRSDDGGKTWETPGGEKMPGPTDPPGGLSNKFVYDTSS
                     ESGKPLTTHQWYDGTQRPWEFKRVWHLEPSLTEPDTVYAGVEDAALFRSTDGGKTWHE
                     LASLRSTKGELWQPGAGGMCLHTVILDRHNPQRIFTAISAAGVFRTDDGGKTWRPVNR
                     GLKSQYELPDPDAEVGHCVHNIAMHPSRPHVLFMQKHWDVMRSDDAGESWHEVSGNLP
                     TDFGFPIAVHAHEPNTVYVVPIKSDSEHFPPEGKLRVYRSRSGGNEWDALTKGLPQRD
                     CYVNILRDAMAVDALDPCGVYFGTTGGQVYGSADGGDTWEPLVRDLPAVLSVEVQTL"
     gene            complement(1547..1903)
                     /locus_tag="VFH05_05050"
     CDS             complement(1547..1903)
                     /locus_tag="VFH05_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6677611.1"
                     /translation="MKYMCLVYVEETILNAMPKEERQSLSDESMAYCNNLIKMGQCLA
                     ASPLHPVETAATVRVRNGKASTTDGPFAETKEQLGGFFLLNVRDLNDAIRMASMFPAA
                     RYGSIEVRPMKEDGCA"
     gene            2257..6279
                     /locus_tag="VFH05_05055"
     CDS             2257..6279
                     /locus_tag="VFH05_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6677612.1"
                     /translation="MLVLFGWAVNSTDLKSLAPGLIPMMPNAAIAFVCMGISLLAASA
                     RTHRQYVRFMAGGAAAVAAIIGLATCLEYLLMVDLGFEQWVFFEPLETVGSSIPGRMG
                     INTAICFLIVGLALALKILAPGRLEAMSDGFSLGATFLAFLAFLGYLYQERSLYGVGP
                     YTPMALHTALTLMLVGAGALALEPAKGFMAVINSRQAGGYMLRRMLPVVFVILPAIGW
                     IQLYGQRKGWYGTDMGVAIFATTATVTLAALLWETARRMNEADQARRQSDRLFAAFMT
                     HLPALAWIKDLQGRYLYFNDAFTRAFGVRLEEWRKKTDYELLPDHTAAQFQANDAQVR
                     ATAKAVMAVETAPYKDGVHQSLVSKFPIVDEEGNLSLIGGVAVDITERNRIETALRET
                     EEHFRQVTEHIQEVFWLSDTAKNSVLYVSPGYESIWGRSCESLYRSPRSWLEAVHPDD
                     RQRVFEAALAKQMTATYDEEYRIIRPDGSMRWIRDRAFPIHDSSGSVCRIAGIADDIT
                     ERKRAEEALQRAHHELERRVAERTAALRASQERLEMAFHGAGLASWDWQIDTGAVSFN
                     ERWAQLRGFAVHELLPHVSSPLNGIHAEDRSMVEMNLNACLRGETPEFEAEMRVQTRA
                     NEWTWILSRGRVIERSAVGAAVRMAGIEIDMTARKRTEEALRESEARYSSLVSQAADI
                     IYTAGIDGRFTFVNAAACAIMGYEEQELLGKHYLELIREDARDRAQQFYKDQLLAQVP
                     STYYEFPAMTKTAEEVWFGQHVRLRLVEGQLVGVEAIARDITARKMAEQALQERAKCA
                     AFAAEVSLLLNHDEPLDRLLQRCTDAAVEHLGAAFTRVWLLKPGDLCGDCYKASSCLD
                     RTTCLHLHASSGLSTNLNGEYRRVPLGALKIGRIAQGDGALFTNDVLHDDRLPNKDWM
                     REHGLHAFAGFALMVEGRVFGVLGLFSCEIISEPIRQTLESVCNGLAASIARKQAVAA
                     LQASETRTRAIVESALDAVVTMDERGIITGWNSQAAAIFGYQEREISGRLLSDTILPP
                     RYREAHSNGLQRFLSSGHGRILNRRVELSALHKDGSEFPIELSVTALTIEGRKVFSAF
                     LRDIRERKQAERALKEAYEQLRDLTGRLTEAEEIERRRLARELHDEFGQALTGLKFDV
                     AWLTKEMSRVNKVPDAAGIKAKTMGMSHAIDGLIKSVRATAASLRPGVLDDLGLVAAL
                     EWLAGSFHERTGLPCDLMIDSSARDVPTDLALATTVFRGAQELLTNVMRHAQASKASI
                     RLTAGEGQMVLTIGDDGRGFTLDQWKQGRSLGLRGLQERVQLVGGSVSIVSSPGAGTE
                     VTLSLPMKSDPVPVVKEERR"
     gene            6417..7112
                     /locus_tag="VFH05_05060"
     CDS             6417..7112
                     /locus_tag="VFH05_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677613.1"
                     /translation="MLSNQPASLSQPQPIESPDQQNVKLKILLVDDHAIVRVGLRQML
                     LDAFHQLDVTEAGTGQEALSKVQQNGWDLVILDINLPDTNGLDVLKKIKTLRPALPIV
                     VLSFHPEEQYAVRALKGGASAYVTKDTAPQELDTAIKTVLAGHQYVTPSLAQRLEAMP
                     IQSGGKPLHTLLSDRELQVLSMIGGGKSVTEIAVALDLSVKTVSTYRMRLLSKLQLKT
                     TSALIRYALTHHL"
     gene            7300..7974
                     /locus_tag="VFH05_05065"
     CDS             7300..7974
                     /locus_tag="VFH05_05065"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HET6677614.1"
                     /translation="MFRRPVSTPSERRGVTQNKRFLLGLLLWYVALSVWTAYEPADRQ
                     FWMLSSILPGLLVLVLVGTYRCLPLSPISYVLITSFLTLHTVGVHYTYAQVPAGAWFE
                     QAVPLGRNQYDRAVHFCFGFLLTYPIQELFRLTIPLRGWLLYYLPVMTVLGLSGLWEI
                     VESWVAQAVHPELGITYLGSQGDAWDAQKDMAAAMYGALLCMAFLFFWRKQRSAASRP
                     LAELES"
     gene            7993..8598
                     /locus_tag="VFH05_05070"
     CDS             7993..8598
                     /locus_tag="VFH05_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HET6677615.1"
                     /translation="MKKTTLLYGLLAWYGLLWTALAIAPRDRQDWLLENLLALAAVAT
                     LVVTYRRFQFSSPSYLLITAFLSLHAVGAHYTYAEVPVGFWLQQGFGLSRNPFDRLVH
                     FAYGLFLAYPLREILVRLAGVKGFWSYYLPLSGMLAQSGFFEIVEALVAQIVSPELGA
                     AYLGTQGDEWDAQKDMLAAGSGALLCMMAVMVAPTQSGKTA"
     gene            complement(8608..9285)
                     /locus_tag="VFH05_05075"
     CDS             complement(8608..9285)
                     /locus_tag="VFH05_05075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6677616.1"
                     /translation="MRLLTTLIVSLLLNAASAIPVRAADPGTEEQKTFYALGLIISQS
                     LAPFALSESELEFVRNGMTDGVLKKTSKVDLSVYGPKVNQVQQTRAAAVAESEKKAGA
                     AYVTKAAAESGAKKTESGAIVTTIKEGKGEQPKATDTVKVHYHGTLIDGTVFDSSVKR
                     GEPATFPLNQVIKCWTESVQLMKVGGKSKLVCPSAIAYGDRGSPPTIKPGATLVFEVE
                     LLEIVKK"
     gene            9448..10860
                     /locus_tag="VFH05_05080"
     CDS             9448..10860
                     /locus_tag="VFH05_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET6677617.1"
                     /translation="MVLTFVIVYLLISVSIGLLAATRVHNTKDFAIAGRSLPVPIVMA
                     TVFATWFGAEAVMGISATFAKEGLRGVVADPFGSSLCLVLAGLFFAPRFYRLNLLTVG
                     DYYRLRYDRRVEVLCTLCIVASYLGWVAAQFKVLGLVLNVVTEGAVSQTLGMVLGALI
                     VLVYTTFGGMFSVAILDFVQLSVIVGGLLYIANVLGDLTGGVGVVVRHAQQAGKLEFF
                     PPASLNAWIPFVGAGVTMMLGSIPQQDVFQRITSAKNERTAVRGSLMGAALYFAFCFV
                     PMYIAYSALLVDPTKFGTLLERDFQLVLPTLIVEHTPVFAQVMFFGAVLSAVMSCSSA
                     TLLAPSVILSENVIRGAMPRMDEKGLLTTMRIVLVCFAFVVLTIALSSDSSIYKLVVN
                     TYKVTLVAAFVPLCAGLFWKRATSGGAVCAIAAGLGCWMGFELLGPAASIWPPQLAGL
                     LMATVGMIAGSYLFPDGSSR"
     gene            10949..13243
                     /locus_tag="VFH05_05085"
     CDS             10949..13243
                     /locus_tag="VFH05_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET6677618.1"
                     /translation="MLHSRWLFRGGLILAVSCVLLVAGLLVYGAYLATFLELPRNEDH
                     PALRLYTAPFQLKAGLSLKVARLPERLQRLGYRAVKAPVASPGDYQLTPEALTIFLHA
                     QPETFVKANVVTLALEDGVVTQVLSEPDRTPIFPVFLEPELISGLRGASRQVREWIPL
                     SQMPPRLVEAILAVEDRRFYSHPGIDPVAIGRAVWRNLTKGGVVQGGSTITQQLAKNL
                     FYTPQRTFARKVKEALAAIVLETKYRKQDILESYLNEIYLGQAGFVSIYGVGEAAHRY
                     FGKNVGELTTGEMAMIAGLVKGPNTFAPTKHRALAQQRRDVVLRLLREQGHVTEHEWK
                     VAVNQPLQVAPPDDVLADAPYFLDTVLRQVEEAIGTPLPEGLRIDSTLDAIVQQIATE
                     GLENGLTRLERAYPALRGSDQLLQGAVAILEPSTGAMLALVGGRDYRVSQFNRAVQAH
                     RQAGSLFKPFVYLAAFEASRTGTPPYMTPASLLADEPVTFESEGVTWAPQNYDKQFRG
                     NVTLRSALEQSLNVPAVRVAKAVGTQSILDVIRQLGVTSALTTNDLSLALGSPAVSLL
                     DMVTAYGVLANRGIWVHPTSLRAISDRDGLVLWTPAPDRRQGVSPQAAYLVTSLMEGV
                     VQRGTAAKAKVLGLTGSIAGKTGTTDGYRDAWFVGYTSELVIGVWVGFDDERAIRLTG
                     AQAALPIWMDIARRMLPEHVDPFTPPSGIVARAIDPKTGQLATSQCPEQVEEVFIEGT
                     EPTVYCEVHGGGIWERLRHRFGFS"
     gene            13351..13794
                     /locus_tag="VFH05_05090"
     CDS             13351..13794
                     /locus_tag="VFH05_05090"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6677619.1"
                     /translation="MKKNGFGGDDNITLLAKGVHLKGEIRVEGTVRIDGRLDGEIHTK
                     GQVIVGEDGVVQGLVMAGTVVSSGRIKATVTAGERVQLLKTAILIGEVHSPVLMMEEG
                     AKMQGTTDMGVAAWPDDLPKLTGTVRELVSHRAKHAVVVEQDSGS"
     gene            complement(13907..14500)
                     /locus_tag="VFH05_05095"
     CDS             complement(13907..14500)
                     /locus_tag="VFH05_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6677620.1"
                     /translation="MDRFRIAYSTAGLTFLILAVWWVASAYPAKDDSKTPGISPERVA
                     GFVHAVLQAHRTIYTTHIVNRLQEKGVVKAVEHWEQENALPLPAQFLQHSGRLVAESG
                     QGIRYRLIGLSPIYQRNAPATDFERKGLDLLNRSPEAPITGIVSSGRKQYFQAIYPDR
                     AVSAACINCHNSHPLSPKRDFKINDVMGGIAITIPIE"
     gene            complement(14452..16968)
                     /locus_tag="VFH05_05100"
     CDS             complement(14452..16968)
                     /locus_tag="VFH05_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677621.1"
                     /translation="MTKATKQPSPVTRGWRLQTKLIVSMLLVGVVPLLVGLGMAFWQG
                     SQEIHEVSGESFKALATEAARKLDLLVGEEIARTSRIAADPMIVRELEQRRDRNLKPE
                     SGRYPIKQDLQLKWESKDPATVKMITENPVANLLREYYTGARSEPDELIPQVVRGATK
                     MLFVTDTQGHLAAALTDKPPFANGEFLWWKGTFNKGIGQLYIEDVYFDELAQTYAFSI
                     SLPVMDSLRYEAIGVIHRVIDAKEFFGPSTHPIRFGKTGHVMLIDHRGIVVSCPILPT
                     GVRLSDASIIPLVTQRQSGWTSAPSDGHGGRSSSIIGFSPLPETSRNTNGALEVGSWH
                     TFVWQSSDELFAPIKHLFTWMTVFGSIAVALLATLGYLAAKRIVTPVRQLQQAAQSIG
                     RGELRQSIEIKTGDELETLAEEFNRMNRQLEAAFTGLTDQVTLKTQEVQYLQRSTDQI
                     LDAVPTPILIVDDQEEVLYVNRTGREAFALTEESSAGRRLFDMLPVEPSQQQHLRQEL
                     WSHQTTGMAPSLAGEPPAAVEHIRDPLVPETATVHRSRQAELQLGAHLYRYEWFRVIG
                     RPGQSDRVGLVLRDTTDESRMQEKLVQAEKSGSLGILTAGIGHELNNPLFGIMGLGEA
                     LQDECDPARVKSYAQDIIHHGKRMAAIIRDFTGIAAREISDQRQTVHLEREMDQALAA
                     VMVGLDTSQLTIERTYAGDTVVMALPDQLKQAFSNIMTNALHAMKNQGTLRLSTTVGE
                     KTVVATIADTGPGIPKNHLSKIFDPFFTTKGQGEGSGLGLTVARRIIRKFGGDIRLES
                     VEGAGTSCMITLPLMAPASSSKEASWTASESRTLPQVSRS"
     gene            complement(16965..18032)
                     /locus_tag="VFH05_05105"
     CDS             complement(16965..18032)
                     /locus_tag="VFH05_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677622.1"
                     /translation="MQPVTDRTVAAKLAPTKVAILGGGRGGTALLDLLHLIPSIEIVG
                     ISDRDPAAPGLLRARNLHIAVTDRIEDLVGNHGVNLIMDVTGDPAMEQFIQLHKQPDT
                     QVLSGSAARLLWELVSHEANLQAELFHAEKLAGIGFFAAGIAHDINNPLQLIMGLAEN
                     LFEETDLKVVHEQAREIIEAVRRTSAICRDLTQYARKTSARDDTMVSLNNRLDEALKI
                     ARYAVSFHDITVDKQYADGVEARGSPDEVLHLLVNLITNAVQAMEKDGGTLSLTTRTS
                     DDGVRLRIADTGCGIAPDLLGQIFDPFFTTKDPGKGTGLGLYNVKTIVTKMRGTIHVE
                     SRLAKGTTFTITFPAVPEERT"
     gene            complement(18097..18531)
                     /locus_tag="VFH05_05110"
     CDS             complement(18097..18531)
                     /locus_tag="VFH05_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6677623.1"
                     /translation="MGEFTSGFFMGDQATNGRILVVDDEADIRKVVRMTLQKAGYDVL
                     EAENGERAIETINSGENRLLLDILICDIRMPKINGVEAIAYFRANYPRVPVIVLTGFP
                     DADMATSMLRQGVVDYLVKPVEGEKLRESVARAMEQRELAHL"
     gene            complement(18674..18832)
                     /locus_tag="VFH05_05115"
     CDS             complement(18674..18832)
                     /locus_tag="VFH05_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677624.1"
                     /translation="MPFTMMPPATTVVISSRLLLRRICLDAAPAAAGGGGLVVDFDLR
                     WWITTDLE"
     gene            19147..19410
                     /locus_tag="VFH05_05120"
     CDS             19147..19410
                     /locus_tag="VFH05_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HET6677625.1"
                     /translation="MITEQTLTEYIRRAMPDAAVTMIDKTGTMDHLKVMIVSDAFQGK
                     NLLDRHRLIYQALDAPMKDGRIHALELTANTKDEHEQRVTGTR"
     gene            19434..19757
                     /locus_tag="VFH05_05125"
     CDS             19434..19757
                     /locus_tag="VFH05_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET6677626.1"
                     /translation="MGDPIEEEIQKEVKGHKIVVYGKGTKQMPMCGFTRETMQFFDKY
                     GYPYEVIDVLSQPAKREALTKMTNWPTLPKVFIDGTFYGDTDILDPMAAKGEIEPLLK
                     KAFGK"
     gene            complement(20029..21771)
                     /locus_tag="VFH05_05130"
     CDS             complement(20029..21771)
                     /locus_tag="VFH05_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677627.1"
                     /translation="MTGYTALRLPAESVFAVLLLGLFPCHGSAGQAHPSQSESPAALL
                     LEARNAALSIQETAERTDALTVIVGKQVQLDPSGARETLKMFPKLPNKLTYLADLAAA
                     FAKAGNIPETERLYAEIVVEDQSSRIGKLAASNALGQLALAYVKSGNIEEAFRIYERL
                     KERTKQEPPAIVGVVAANLVEAQAKQDDIPGALRTYSGIAGENPYPLMKIVGDRARNG
                     KLSEAQEIVGRVDDASQRYAQWGIVQAQITQGRLTDAQVTASGIKPGHAKASALLELA
                     QYHRQHGANQLALLLLQEADTSARSTVNVWTRADILRRIAAETAIVGDPATAVNIAKS
                     IEKDGHRNSALHDIAKAQAKRGDIAGAFNTAAMVKRAPQADDLGRTVYETVASDILVE
                     MVKTGKGKEARETAAHFQDTDVSRAWLYSGIAMAQADLGHIKEAHAVLALAETEAQRS
                     ARRKELRQVEEKIRLGSQTTADDSRRRELVKMELDISRALEAMAKALARKGDLSAAIA
                     LARESNEPAQRLKLIEALSAVHAQSGYKLITVRWARTLSSPSERAFALVGIATGLSRE
                     ADKPKAKAAASQTR"
     gene            complement(21768..22172)
                     /locus_tag="VFH05_05135"
     CDS             complement(21768..22172)
                     /locus_tag="VFH05_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677628.1"
                     /translation="MKKEQDRQAHHRFLQALQHEHLTCTNGACGGPMEITDLTPHHAR
                     VKTYEAVCERCHQKEQIAGKEETSPPWDTATITMMAEVHLLHDQPTCPFDDTPITFIS
                     MPNPRRKARYRLLCYYCGRHTEMNWPPPEAKR"
     gene            complement(22295..23029)
                     /locus_tag="VFH05_05140"
     CDS             complement(22295..23029)
                     /locus_tag="VFH05_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6677629.1"
                     /translation="MTMKVALITGGAKGIGRGIALDLASQQWHIALCYRTSEAEANDT
                     AAAIIRAGGQASAIRCDVSDPVAAKEMVALVEKQWGHIDALINGAGPYHRVNLFEETV
                     TGWNEMFDGNLHPIFYLAQAAAPGMKARRFGRIINFSMANADHMIGQPDVTAHYIAKA
                     GVLILTRTLAKLLAPHGITVNAISPGFIDSGSAPPGELTGMAKRIPAGYIGSVEDTVA
                     AVRYLLSPEARYVNGANIHISGAWGI"
     gene            complement(23043..23789)
                     /locus_tag="VFH05_05145"
     CDS             complement(23043..23789)
                     /locus_tag="VFH05_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET6677630.1"
                     /translation="MKPMPTHPQPARTALVIFAKAPVPGQVKTRLCPPLTADEAATIH
                     GTFVLDTLERTKAAVSRLKLQADRYLACAPSSALAFFRILEERQAVGLLEQEGEDLGA
                     RMHRACETLFQRNYQQVLIVGTDVPSLPLDYYRQALDQLERHDVVVGPALDGGYYMIG
                     LKKIAPALFQGIPWSTDRVLSMTREKAIAMGLRVALLPEWRDVDTIDDLRLLIDASIL
                     DAGKPKQEQSFSNRTAGALQLIAKRLRSRT"
     gene            complement(23801..24574)
                     /locus_tag="VFH05_05150"
     CDS             complement(23801..24574)
                     /locus_tag="VFH05_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET6677631.1"
                     /translation="MSTLRTSSSSPWEPAHLAHRRAAVTVGVIIPTLNEASVIETTLQ
                     LTRQLGFEEILVVDGGSTDRTNVVVDMVAGSPNAGSTPVRRLAAPAGRSMQLNAGARD
                     CRQDVLLFLHADSHLPVDAKRLIQFALADPEVVGGRFDLRFDRPSMWGRIISLFMNLR
                     SRLSRISTGDQAMFVRRQVFQQLGGFPGIPIMEDIELSARLKRTGRTVALRGFVTTSF
                     RRWERRGPLRTILLMWALRFLYWIGISPHRLARLYADVR"
     gene            complement(24598..25014)
                     /locus_tag="VFH05_05155"
     CDS             complement(24598..25014)
                     /locus_tag="VFH05_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677632.1"
                     /translation="MAPTDLPTPQDPQRSSPKPPQSPSDSQESSGDHQMRVIGLMVGT
                     ALMFIGFLNVFLSISGGFEINVVPFLIYFGGLAVWANAAIEMPAVRYGVMAAAIGLAL
                     GFFHYGEVLFWHKQVVFWATVIIVMYFMFNEPKKPG"
     gene            complement(25113..26267)
                     /locus_tag="VFH05_05160"
     CDS             complement(25113..26267)
                     /locus_tag="VFH05_05160"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HET6677633.1"
                     /translation="MTRRPAQGILPYQQITRLITAGTISAPTPIEDRQIQPASLDLRL
                     GKKAYRLISSFLPELSAISSRLDVMDFYQSDLVMYEMDLSGGAILEKGHVYLVPLLES
                     LRLPSTLRARANPKSTTGRLDVFTRIVTDLNAGFDEIRAGYQGPLFLEVVPRSFAIKV
                     KTGQSLNQIRFVRGEAAASDAALQHLHRQEPLLYHNLSDRKPLRQREFRTDRGLFLRI
                     DLKGDERTDSKVIGYRAKKNSHVIDLSKIGYYAATDFWEPLYRHRQDSLLLEPEEFYI
                     LASKERIRVPPDYAAEMVAYEAACGELRTHYAGFFDPGFGYGADGELKGTQVVLEVRP
                     HDVPFLIHDGQTFFKVVYDRMLDVPERLYGVGLGSSYQQQALTLSKHFKA"
     gene            complement(26264..27442)
                     /locus_tag="VFH05_05165"
     CDS             complement(26264..27442)
                     /locus_tag="VFH05_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HET6677634.1"
                     /translation="MEDWRRILADSIVKPKDLAERFGMDEQEIEAIVGPYPMRITPTV
                     LGTIKEKGDPIWKQVMPDPAELTDIDAEDDPLEEDLMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDQAIAYLQAHTEVRDVILSGGDPLLLPDHLLER
                     ILKALRTITHLEVIRLGSRVPGTLPERITPKLCSIVKKYHPVYMNLHFNHPDELTPAV
                     KEACGMLADAGVPLGAQTVLLKGVNDDPETMKRLVHQLLLARVKPYYLYQADLTKGTN
                     HFRTTIESGLNIIKALQGHTSGMAVPHFVIDAPGGGGKIPLLPDDYIVHMDEDGAILK
                     NYENKTFHYPQPKAGNGRELPMVGARTSYDDSSCADSYPERDGYSSWGNSCGGEIDDL
                     "
     gene            complement(27549..27803)
                     /locus_tag="VFH05_05170"
     CDS             complement(27549..27803)
                     /locus_tag="VFH05_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET6677635.1"
                     /translation="MTMTLYHVQWCPECDVVRRKLAELNLPYEDVIVPDFRPQRKEVF
                     AVSGQYYVPVLQDGETVLSDTGEILAHLENKAESRRLANG"
     gene            complement(27800..29311)
                     /locus_tag="VFH05_05175"
     CDS             complement(27800..29311)
                     /locus_tag="VFH05_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677636.1"
                     /translation="MSRTDSLSDLATSDLLYRCSSIRDALIEAGLFAPQPVSSSLTAP
                     DEDSASASWRIGLEPLPLTREQMVFFEALGPQLLSFYKALNRLYTESAKGTTQPGWIA
                     DWLDQGKPEALIRYSRMKRFRDELPGVIRPDVIPTQDGMVITELDSVPGGIGLTACMS
                     RLYESQPASSESDNATAGAQLVGGPDGMLNGFAGMLRSYRGEQEGVVAIVVSDEAKDY
                     RPEMGWLAKELTAGGLETYCIQPRDIRFTEGKLFLVTEGGDRPIGLIYRFYELFDLPN
                     IPKNELIQYAAKKSWVSITPPYKPALEEKAAFGLLHHPTLVPFWRRELGEEVYVHLST
                     VMPRTWVLDPAPVPPTAVIPGLSVGGRAVTNWSDLAEATQKERHLVIKPSGFSELAWG
                     SRGVSVGHDLPQSEWTAAIDSALRSFPHTAYVLQEFHKGRQYHLEYFDPVTADIHAMA
                     GRARLSPYYFVTGEKVDLAGILATVCPADKKVIHGMKDAIMVPCAAVQGKATE"
     gene            complement(29343..30356)
                     /locus_tag="VFH05_05180"
     CDS             complement(29343..30356)
                     /locus_tag="VFH05_05180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6677637.1"
                     /translation="MALPPEGPQYDPQVLLNSQPVIITVIDPLTHRVVFQNQTSQNKF
                     GDISNLTCHEKIANCPTPCAFCKMPEAVDTGKPTASEVPLPNDEYLLVQWAQVQTANG
                     KTHIVETITDITATKRQQAEAEGLVKRLSNTNRDLMQANQLLRDQSIRDSLTGLYNHS
                     HFQETLVHLCSLALRSMRPLSLLFIDLDNFKQINDAHGHTMGDQVLREVGWLLDSQQA
                     PGRGTARSSDFSARYGGEEFALILPDTSMDGALSAAERLRHRVTTLTMLPELTGLNTH
                     PLALTCSIGVATFPIHASIPSDLVVAADAAVYAAKTAGKNCVRLASPANPAAPLFAPL
                     VGS"
     gene            30473..31972
                     /locus_tag="VFH05_05185"
     CDS             30473..31972
                     /locus_tag="VFH05_05185"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET6677638.1"
                     /translation="MAALHAKNVMALTGAARGLPQAVPNSACFHCDVCCRFPDPDSVL
                     RPYFTDEEIASAVDRGLKASAFPDLNGSQVTLVPNPQGEGFLCPAFESTTGRCLIYDQ
                     RPLDCRLYPLVLMWNASHDQVVLGWDSTCPYTKEHVPDSIRRHADQVLAVLEEQSTVQ
                     RIADHPRLVGRFQSEALVLAPLPGLTRAISTRWGPEQCRRLVLEDLSRLKTALDRSKI
                     CGSLAAYSAPYHYMWNALLPYWWMDLEGALCLFIQSPDGWFMPLPPLAEGALTRPLNK
                     AFDLMRQWNGASAVSRVENVPAVLVPTLVEMGYRVLPKSPDYVYRADDLARLAGDRYK
                     SQRALCNRVEREGGMVVELFRPQDRAACRALLHKWRSQKLARGADPLAELLLEDSVSA
                     HEVAWSHASELQLTGSVLRRNGTLCGYTFGYWLDRQTWCVLLEVTDRTIPGSAQFLFR
                     ETCRHARAEGAEFINTMDDSGLTGLRLSKEAYHPAARIDSFVCSEILQP"
     gene            31969..35463
                     /locus_tag="VFH05_05190"
     CDS             31969..35463
                     /locus_tag="VFH05_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6677639.1"
                     /translation="MSQQDFQQPLDPPRYRWLPHLIILMNLVAVAAGVFLLRSVEQRL
                     VYSAGEELRIAAAEVADKLDRLLFERYGDAHMMAQALALRPSDTAYLTSYVGWMKTNH
                     APVYAWLGVTDSRGVMIAATESSLRGQDFSRTGWFQRVSQSKTIQVDDVALHEGEGET
                     ESVAFSAPMLDAEGNLSGIVSSRVPVSVLEEVTTGTLRSLEARPEFAGAVVFQMVTDQ
                     GRVFVDSDLRRNRAFSQRDSGALSSRAAPLGDLGFVEEQSHGQAIVTGFARTKGFGQF
                     PGMGWHVLVRMDRDRILEPILSILWKVSLMGAVVWVPMVVLLFWSTSHLRAEYRQVQQ
                     ESSWARAAEAALLQSQERTRAIVETALDALVTTDAAGIVSDWNGQASAIFGWSREEAL
                     GKRLTELIIPQRDREHHDRGIRQYLASGETHILNRRIEVQALRRSGQEFPVELAISPV
                     RVGDTIFFSAFIRDITERREADTKLKESEVRYRSVVNALDEGVVVIDASGFLRTGNAS
                     AERILGVSGEELTGRTLRDKQWRTIREDGSPFPPEQYPAAITLDTGEPCAGVVMGIYR
                     PDGQLRWVEINSRPLTPDGGASPAAVVISFSDITERKRVEERLTAQYAVTRVLAESRS
                     LEDAVPKIMRAVGQNLEWDCGIFWRVDRTTSVLRCLDQWAGAGVEAQSFLEKTWDLTF
                     KQEEGLPGRIWSSGKAAWITDVARDANFPRRLLANEVGFHGAFGFPVRVGGDIEGVIE
                     LYSRQVRVPDNELLKMIDDVGLKLEQFGDRTRAQEALQQTEAQLRQSQKMEAVGRLAG
                     GVAHDFNNLLTVIRGYSELLLARLPADDPIRKDMTEIKKAADRASGLTRQLLAFSRRQ
                     FIAPKVIDLNALIANMDGMLRRLLGEDIVELCTELNPHTGWIRADAGQVEQVIMNLAV
                     NARDAMPKGGRLTIETRNVTIGQPTRQTPAGVKPGAYVLLAVRDTGHGMDEETQSHLF
                     EPFFTTKEKGKGTGLGLSTVYGIVKQNGGSILVESQKDQGATFKIYFPLVEQGAPGMV
                     APDAEPVHGRETILLVEDEPSVRGLVHETLRLHGYTVLEARHGIEALMTGAKHQGPIH
                     LLLTDVVMPQMSGPEVAEKIQIVRPGIKVLYMSGYPDHPVFEQGGISRDASFLPKPFS
                     PNVLAKKVRDVLDGGKVA"
     gene            35641..36459
                     /locus_tag="VFH05_05195"
     CDS             35641..36459
                     /locus_tag="VFH05_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HET6677640.1"
                     /translation="MTQQGREFDMSQYRIEKEPYYADVRGEVDLFLTAAKSRMPVMLK
                     GPTGCGKTRFVQYMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWVDGPLTLGV
                     KQGAIVYLDEVVEARKDTTVIIHPLSDDRRLLPIEKKGQAIEAAENFLLVISYNPGYQ
                     NVLKDLKPSTKQRFVAIEFDYPTADIEASIVEREAGIAHDLAVRLVKLGGKIRHLKNH
                     GLEEGVSTRLLIYAGTLIREGIAIERACDVAIARPITDDADMQRSIMELVKAMF"
     gene            36588..36878
                     /locus_tag="VFH05_05200"
     CDS             36588..36878
                     /locus_tag="VFH05_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HET6677641.1"
                     /translation="MKDWQGGILLTVFAQPKASRTAWVGLHGDAVKIRIAASPVDGAA
                     NRELIRFLAEELSMPMSAIDIHSGAGGRHKRVWVRGTTAVDVTAYLIRKCQG"
     gene            36881..37306
                     /locus_tag="VFH05_05205"
     CDS             36881..37306
                     /locus_tag="VFH05_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HET6677642.1"
                     /translation="MNLFRALVLMVGTLLCACSGPKPVLYPNAHLQSVGNETAEQDIE
                     ACKEAAETAGADEGSGKAGRVATSTGVGAGVGAASGAVGGAISGAAGQGSLIGAASGA
                     VWGLLTGLFTAGSSQPSQAFTNYVNRCLQERGYEVTGWQ"
     gene            37401..37823
                     /locus_tag="VFH05_05210"
     CDS             37401..37823
                     /locus_tag="VFH05_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677643.1"
                     /translation="MNKILISWLVIALAGCSGPEPMLRANAKLLLKGREASHLEVAAC
                     QQKAEAAGLQPGTAGRAGNMAAGAGLGLIAGAAVGASTGVIGGATGVAIGAAAGAGLG
                     LIIGLAGGAYRPLEPDPPYADAVVRCLIEQGYEVTGWH"
     gene            complement(37825..38238)
                     /locus_tag="VFH05_05215"
     CDS             complement(37825..38238)
                     /locus_tag="VFH05_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HET6677644.1"
                     /translation="MAEPKQASLDKMWKYVNSFAAKSGTTMHPNRAVTESVVKGLAMH
                     VDELGKPLCPCNFYKDKAAEAKQRRWICACDEMQIYKYCHCLLFVREDGMPITEYLPE
                     GHEGREVYGVITDPTPDKGRALRHKASDASPDTTD"
     gene            38467..>40519
                     /locus_tag="VFH05_05220"
     CDS             38467..>40519
                     /locus_tag="VFH05_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677645.1"
                     /translation="MESANLQLINRLAGELDAVEAAALVPRIVAAAHKPAAILALLDE
                     LEAISAKAVRAAIEALPELDRRAGCSQIHAWLDLGVALAGSSGAIALKYFKDSPLIFG
                     LIERSDRQAAVLAMGLELAEHDANVSWEYLKTAPQIATALPMDQWPAWLDIGLEMTRL
                     EPVVGLEYIRQIPALVPVLGAPDVRQWLGFGMKLIMPNSLGKPDYLATMEFLRTSPAI
                     LSDIEAPLRSSVISVGALLAERAPETGIAWLAESPRLLRLLPSPPWRTKVVQYGALLA
                     EKDADAALSYLRRGPELVVLIGEGPQTDARFEEWFRAGMEVLEYSPEGARAYFATESL
                     KALSSVEHALSGVPLRQVARQIKLVVQGLCGSDVSIIARPEALTTAIIRPIVSADGKT
                     IALPALLRRYPTARQNERLYLVMAAHEAGHLEFGTYALSMTSLLDLLEAVCRRYGRPR
                     PATLESLAALFRLYPHPRLMQDLWTILEDARVESFLQREYPGFRRDLAQFAADTILPR
                     DPAHGLMVRELIADCLLRLSVGESMSSAVPRAVMEEVSILWDLCQSVLTADSTAERSV
                     RTAHAVYVRMEELLASRADLLESEQPADDSTETASGQSAPEQADNDYRPVSNAFYRGE
                     MHPEMIGGDVEKSDSEVQPPGGGSTERIPAERGRFGEEHSLGGDLLGGGHSLPAVVEE
                     LL"
BASE COUNT         8146 a        11413 c        12109 g         8851 t
ORIGIN      
        1 tgagaaaggt cctgctcaca cgcgaagaac ctcacaaagg cccgccgccg ttttgtgacg
       61 tgatctcgga tggttccctt cagcatcgga tagccgtcct caaccgcgtc cagaactgag
      121 cgctgtgtca ccggtccaca aacgttgacg gtgacctccc cggcgacgcg cgccaaagtg
      181 cgcaagtgtg cggggaggac gactcgtatc atcgtgtgca ttgtccctta ttgcctatta
      241 aaaaacagca tcgccggact cttcaacggc tttcgcgcct gctgacatct gtggatcagg
      301 ttacagggtc tggacttcca ctgaaagaac cgccggcaga tcccggacaa gaggctccca
      361 tgtgtcgccg ccatcggcgg atccgtagac ctggccgcca gtggtgccga agtacacacc
      421 gcagggatcg agtgcatcca cagccatggc gtcgcgtaag atattgacat agcaatcacg
      481 ctgcgggagt ccttttgtca gcgcgtccca ttcattgccg cctgaacgac tccggtacac
      541 ccgcaacttt ccctccggcg gaaagtgctc cgaatcactc ttgatcggta ccacgtagac
      601 ggtgttcggt tcatgcgcat gtacggcgat agggaaacca aagtccgtcg gcaggtttcc
      661 gctcacctcg tgccaggatt ctccggcatc atcgcttcgc atgacgtccc aatgcttttg
      721 cataaacagg acatgaggac gcgaaggatg catcgcgatg ttgtgcacgc agtggccgac
      781 ttctgcgtcg ggatcgggaa gctcgtattg cgatttcagg ccacggttca ccggtcgcca
      841 ggttttacct ccatcatcgg ttctgaacac gcccgcggct gaaatggccg tgaagatcct
      901 ttgcgggtta tggcggtcca gtatgacggt gtgtaggcac atgccgccgg cgcctggttg
      961 ccagagttct ccttttgtgc tgcgcaaact cgccagctcg tgccatgtct tccccccgtc
     1021 ggtcgagcga aacaaggccg cgtcctcaac gccggcgtag acggtgtcag gctcggtcag
     1081 cgatggttcg aggtgccata cccgcttgaa ttcccatgga cgttgagttc cgtcatacca
     1141 ctggtgcgtc gtaagaggtt ttcctgattc agaggacgtg tcgtagacaa acttgttact
     1201 gagtcccccc ggcgggtcgg ttggaccggg catcttctca ccgccgggcg tctcccaggt
     1261 tttgccgccg tcatcggagc gttgaatgat ctgaccgaac cagctgctgc tctgcgaggc
     1321 atagagccga tttggatctg caggcgatcc tttgaggtga tacatttccc acccggcaaa
     1381 atgggggccg ttgatgtccc actgttttcg cttaccatcc gccgtcaata taaatgcgcc
     1441 tttcttcgtt cctaccagga cccgtacacg gctcataggt cacacctttc cttcctgtag
     1501 tggatcatga tgcggataca ttcatcgagt ggcactgccg ggtcattcag gcacagccgt
     1561 cctctttcat aggccgtacc tcaatgctgc catagcgggc cgcgggaaac atcgatgcca
     1621 tccgaatcgc atcgttcaga tcccgcacat tcagcagaaa aaatccaccc agctgctcct
     1681 tcgtctcggc aaagggaccg tcagtggtgg acgccttacc gttgcgaacg cgcaccgttg
     1741 cggcagtctc tacggggtgg aggggcgagg cagcaagaca ctgtcccatc ttaatcagat
     1801 tgttacagta ggccatcgat tcatccgaga gcgattgccg ctcctccttc ggcatcgcgt
     1861 tcagaatcgt ctcctcgaca tacactagac acatatattt catgactgcc tcctgggtgg
     1921 tgatgatgcc ctgacagaac atagtcgtct tgggccgtca aaatcgacag cggacgcggg
     1981 tccttcttga gagcgaggcc ttctatggtg attcaagcgc gaagtcaatc gcaacatggc
     2041 tacaggctgg gtcggagagg cccaaattcg ttcaccatgt tcaacacgtg tcatataggt
     2101 acctacagaa ggacccttat cttgacaatc ttcatgtttc ctttcatcct tcgccaacct
     2161 catacgcgag ttgatgtgac tcttttgacg gcacttcgtt cgcgtttcgt ccaccctctc
     2221 cctccattgg cgggtcttct atcgcttgaa gtaggcgtgc tcgtcctctt tggctgggcc
     2281 gtgaacagca cggatctcaa gagcctcgct cccggtttga tccccatgat gccgaatgcc
     2341 gccatcgcgt tcgtctgtat ggggatctcc ttattggccg catccgcgag aacccatcgg
     2401 cagtatgtgc ggttcatggc gggcggagcg gccgcagtgg ccgcgatcat cggcctggcc
     2461 acgtgccttg aatatctcct catggtcgat ttgggcttcg aacagtgggt gttttttgag
     2521 ccgcttgaaa cggtcgggtc atcgatacca gggcgaatgg gcatcaacac ggccatctgc
     2581 tttctgatcg tgggtctggc gcttgccttg aagatcctcg cacccggccg tcttgaggcc
     2641 atgagcgacg gattctcttt aggcgcaacc tttttggcgt tccttgcgtt tcttgggtat
     2701 ctctatcagg agcgatccct ttacggggtc ggtccgtaca cacctatggc cttgcatacc
     2761 gctctcaccc tcatgctggt gggcgcaggc gcattggccc tggagccggc caagggattc
     2821 atggccgtga tcaacagtcg tcaagccggc ggatatatgc tcagacgcat gctccccgtc
     2881 gtcttcgtca tcctgccggc gatcggttgg attcagctgt atgggcagcg caaaggatgg
     2941 tatggcaccg acatgggtgt ggccatcttt gccacaacag ccacagtgac gttagcggcc
     3001 cttctgtggg agacggcccg caggatgaat gaggcggatc aggcgaggcg gcaaagtgac
     3061 cgacttttcg ctgcattcat gactcacctg ccggctcttg cttggattaa ggacttacag
     3121 ggccggtatc tgtatttcaa tgatgccttt acccgggcct tcggcgtccg tttggaggag
     3181 tggcgaaaga agactgatta tgaacttctg ccagatcaca ctgccgctca gttccaagcc
     3241 aacgatgcac aggtgagagc taccgccaag gcggtgatgg cagtcgagac cgctccctac
     3301 aaggatggag tacatcaaag cctggtgagc aagtttccga tcgtcgacga agaagggaac
     3361 ctgtcgctga ttggaggggt ggcggttgat atcacggaac gcaatagaat cgagacagcg
     3421 ttgcgggaaa ctgaagagca cttccggcag gttaccgagc atatccagga ggtgttttgg
     3481 ctgagcgata cggcgaagaa tagcgtcctc tatgtgagtc ctggatatga atccatctgg
     3541 ggacgctcct gcgaaagtct ctatcgttcg ccgcgatcct ggctggaggc ggttcacccc
     3601 gatgaccggc aacgcgtctt tgaggccgcg ctcgccaaac agatgacggc gacctacgat
     3661 gaagaatatc gcattatcag gcctgacggc tcgatgcgat ggatacgtga tcgggccttt
     3721 cccatccatg acagttccgg atcggtctgt cgcatcgccg ggatcgccga tgacatcacc
     3781 gaacgcaaac gtgcggaaga agccttgcaa cgagcccacc acgaattgga gcggcgggtg
     3841 gccgagcgta ccgctgcgct gcgagccagt caggagcggc tcgaaatggc gttccacggc
     3901 gcgggtttgg cttcgtggga ttggcagatc gacaccggcg ctgtgtcgtt caacgagcgg
     3961 tgggcgcagc tgagagggtt cgcagtccat gaattgttgc cgcacgtctc ttctcctttg
     4021 aacgggattc atgccgaaga tcgttcgatg gtcgaaatga atctcaatgc ctgtcttcgc
     4081 ggtgaaacac cagaattcga ggcagagatg cgggtgcaaa cgcgtgccaa tgaatggact
     4141 tggatcttga gcaggggacg ggtgatcgag cgcagcgctg tgggcgcggc ggtacgaatg
     4201 gccggcattg aaatcgacat gaccgcccgc aaacggacgg aagaagcgtt acgcgaatcc
     4261 gaagcacggt acagttctct tgtgtctcaa gcggcggaca tcatctatac ggccggaata
     4321 gacggccgat ttacgttcgt caatgctgct gcctgcgcca tcatgggcta tgaagagcag
     4381 gaattgctcg gtaagcacta cctcgaattg attcgcgaag atgctcggga ccgggcacag
     4441 cagttttaca aagatcagct gttggctcaa gttcccagta cctactatga gtttcctgcc
     4501 atgaccaaga cggcggagga agtctggttc ggccagcatg tacgattgcg tctggtggag
     4561 gggcaactcg tcggcgtgga ggccattgcc cgcgacatca ccgcgcgaaa aatggccgag
     4621 caggcgctgc aggaacgtgc taaatgtgcc gcctttgccg ccgaggtcag tctgctgttg
     4681 aaccatgacg agccgctgga ccgcctgctg caacgctgca ccgatgccgc cgtggagcat
     4741 ttgggtgcgg cattcacccg ggtgtggctg ctcaagcccg gtgatctctg cggggattgt
     4801 tacaaggcgt cgtcatgtct tgaccgcacg acgtgcctgc atttgcatgc gagttcaggg
     4861 ctgtcgacga atctgaatgg cgaatatcgt cgtgttcccc tcggagcgct caagatcggc
     4921 cgcatcgccc aaggcgacgg ggcactcttc accaatgacg tgctgcacga cgatcgattg
     4981 ccgaacaagg attggatgcg tgaacatgga ctccatgcgt ttgccggatt tgccttgatg
     5041 gtggaagggc gggtgtttgg tgtcctgggc cttttcagct gcgagatcat ttcggagccg
     5101 atccgccaaa cgcttgagtc cgtgtgtaac ggccttgcgg cgtccattgc gaggaaacaa
     5161 gccgtggcgg cattgcaggc cagcgaaacc cggacgcggg ccatcgtcga gtcggcattg
     5221 gacgctgtcg tcaccatgga tgaacgggga attattaccg gatggaattc gcaagcggcg
     5281 gcaatcttcg gctatcagga acgtgaaatc agcggcagat tgctgagcga cacgatcctg
     5341 ccgccgcgct accgcgaggc tcacagcaac ggactgcaac gcttcctctc gtcgggccac
     5401 gggcgaatcc tcaataggcg ggtcgaactg tctgcactgc ataaggatgg aagcgagttt
     5461 cccattgagc tctccgtcac cgctttgacg atcgaaggcc ggaaggtgtt tagcgcattt
     5521 ctccgtgaca tccgcgagcg caaacaggcc gaacgggccc tgaaggaggc gtacgagcag
     5581 cttcgagacc tgactggccg attgactgag gcggaggaga ttgagcgacg gcgcttggcg
     5641 cgagaactgc atgacgagtt cggccaggca ctcaccggct taaagttcga tgtagcctgg
     5701 ctgaccaagg aaatgtcccg tgtgaacaag gtgcctgacg ccgccggcat caaagccaag
     5761 accatgggga tgtctcacgc cattgatgga ctgatcaagt ccgttcgggc aacggccgcg
     5821 tcgttgaggc ctggtgtctt agacgacctc ggcctggtcg cggcgttgga atggctggcc
     5881 ggctcgtttc atgaacgcac aggtttgccg tgcgatttga tgatcgactc gtccgcgcgg
     5941 gatgtgccca cggatttggc attggcgacc accgtgtttc gcggagccca agagttactg
     6001 acgaacgtga tgcggcatgc ccaggcatcg aaggcctcaa ttcgattgac ggcgggagag
     6061 ggtcagatgg tgttgaccat tggggatgac ggacgtgggt tcactctgga ccagtggaaa
     6121 caaggccgct cgcttggtct gcggggactg caggaacgag tccaactcgt gggcggtagc
     6181 gtttcaattg tgagttctcc cggcgcaggc acggaagtca cgttgtcgct gcctatgaag
     6241 agcgaccctg ttccagtggt caaagaagaa cggcgctgaa aaggacgcgc gtcagcccct
     6301 acacgaaacg ctgtaggaga taacttacac gcaaatgttt ttgttcactt tctagtcgaa
     6361 tttcgctaaa gtgtcgcccg agacagtccg atcgaccatg cagtgaagtc cacataatgc
     6421 tgtccaatca acccgcttcg ctttcacagc cgcagccgat tgagtcgccc gatcagcaaa
     6481 acgtgaagct caagattctt ctcgtggacg atcatgccat cgtcagagtc gggctcaggc
     6541 agatgttgct ggacgccttc catcaactcg acgtgacgga ggccggaact ggtcaggagg
     6601 cgctttccaa ggtacagcag aacggctggg atctcgtgat actcgatatc aatcttcccg
     6661 ataccaatgg actcgatgtc ctcaaaaaaa taaaaaccct ccgacccgcg ctacccatcg
     6721 tggtactcag ttttcatccc gaggagcaat atgccgttcg cgcgttgaaa ggcggcgcgt
     6781 ccgcttatgt gaccaaggac acggcaccac aagaacttga tacagcgatt aagaccgtcc
     6841 tcgcaggaca tcagtatgtg acaccttcat tggcgcagcg gctcgaagcc atgcccatcc
     6901 agtctggagg caagccgctt cacacgttgc tgtcggatcg ggaactgcag gtgttatcga
     6961 tgatcggcgg cgggaaatcg gtcactgaaa ttgccgtagc cctcgatctg agcgtgaaaa
     7021 cggtcagcac ctatcgaatg cgactgctgt cgaagctcca attgaagacg acatctgccc
     7081 ttattcgtta tgcactgacc catcatctct agcgggcgtg caagccctcc tcgcgcctca
     7141 ctgtgcccat tcaatcaagt cacgggacca cggcctcgtt gtttgccttg tcagagctta
     7201 gggagtcacg gccgcacact ctgagaccct gcccccttac cgccggtaag gcagatccta
     7261 ggttgcatca actccgatgc acgctgtaca ttgacggcca tgttcagacg acctgtctca
     7321 actccctcgg agaggcgtgg ggtcactcag aataagcggt tcctcctagg tctgctgctt
     7381 tggtatgtgg cgctttcggt ctggacggcc tatgagccgg ctgatcgaca attctggatg
     7441 ctgtcgagca tcctgcccgg ccttctggtt ctggtgctcg tgggcaccta ccgctgcctg
     7501 ccgctttcac caatatcgta tgtattaatc acgagttttc tgaccttgca cacagtcggc
     7561 gtccattaca cctatgcgca ggtcccggcg ggagcctggt tcgaacaggc ggttccgctc
     7621 ggacgtaacc aatatgatag ggcggtgcat ttctgtttcg gtttcttgct gacctatccc
     7681 attcaagaac tttttcgcct gacgattccg cttcgcgggt ggctgctgta ttatcttccc
     7741 gtgatgaccg tcctggggct cagcgggctg tgggaaatcg tcgaatcgtg ggtcgcgcag
     7801 gcggtccacc ccgagctggg catcacgtat ttgggctctc agggtgacgc atgggatgct
     7861 cagaaagata tggcggcggc gatgtacggc gcattactgt gcatggcctt cttattcttc
     7921 tggcggaagc agcgttccgc tgcttcacgc ccgctcgccg aactcgaatc ctaagcaaca
     7981 ggaagagatg tggtgaagaa gacgacgctg ctgtatgggc tgttggcctg gtatggcctg
     8041 ctctggaccg cactcgccat tgctccccgc gatcggcaag attggctgct tgaaaacctc
     8101 ttggcgctcg ccgctgtcgc gaccctggta gtcacctacc ggcggtttca attctcaagc
     8161 ccgtcctatc tcctcatcac ggcgttctta agcctccatg ctgtcggtgc gcattacacg
     8221 tatgcggaag tccccgtggg gttctggtta cagcagggat tcggactgtc ccgcaatccg
     8281 ttcgatcggt tggtccactt cgcctacggc ttgttcctcg cctatccctt gcgcgagatt
     8341 ctggtccggc tggccggcgt taagggcttc tggtcctact atttgccgct cagtggcatg
     8401 cttgcccaaa gcgggttctt tgagattgtg gaagcgctcg ttgcccagat cgtaagtcct
     8461 gagttgggcg ctgcctatct cgggacccag ggagacgaat gggatgccca gaaagacatg
     8521 cttgccgccg gttccggcgc actcctgtgc atgatggcgg tgatggtcgc accgacgcaa
     8581 tcgggaaaga ccgcatgatg gcggagctta ctttttcaca atctccagca attccacctc
     8641 aaagaccaat gtggccccgg gcttgatggt cggtggtgaa ccgcgatcgc cgtaagcgat
     8701 ggctgaggga cagaccagct tgcttttgcc gccgaccttc atcaattgca cgctttccgt
     8761 ccagcatttg atgacttgat tcagtggaaa cgtcgccggc tcaccgcgtt tgacggagct
     8821 atcaaagacg gtcccgtcga tgagcgtgcc gtgatagtgc accttgacgg tatcggtggc
     8881 tttgggctgc tcgccttttc cttccttgat cgtcgtgacg atcgccccgg attcggtttt
     8941 cttcgctcct gactcggcgg ccgccttggt gacatatgcc gcgccggctt tcttttcact
     9001 ctcggccacc gctgcggctc gtgtctgctg aacttgatta accttgggac catagacgga
     9061 aagatcgacc ttggacgtct tcttgaggac gccgtcggtc atgccatttc tgacgaactc
     9121 cagctcggat tcgctcaacg cgaacggggc gagcgattgg ctaatgatca agccgagcgc
     9181 ataaaaggtc ttctgttcct cggtcccagg atcggcggcg cggaccggta tagctgaggc
     9241 ggcattgagg agtaggctca caatgagggt cgtcaggaga cgcatgcaca catcctttct
     9301 gtatggagaa atcggtgttt caaatgctgt gcttcaggta cagtaagcca tcagcgatgg
     9361 cgggctcaag acaattattc ccgggttgcg cctttttccg tgcaacggta cactgcgctt
     9421 ccagcctcat ccaccatcga ctcctgaatg gttctgacgt tcgtcattgt ctatctgctg
     9481 atttcggtca gcatcggcct tcttgcggcg actcgcgtcc acaacaccaa ggattttgcg
     9541 atcgccggac gcagtctccc cgtgccgatc gtcatggcca ccgtctttgc gacgtggttc
     9601 ggagcagagg cggtcatggg catttccgcg acgttcgcca aagaaggact gcggggcgta
     9661 gtggcggatc cgttcggctc aagtctttgt ctcgttctcg cggggctgtt ctttgcgcct
     9721 cgcttctacc ggctcaatct gttgactgtc ggtgattact atcgactccg ctacgaccga
     9781 cgagtggaag tgttgtgcac cctgtgcatc gtggcatcgt atctggggtg ggtcgctgca
     9841 cagttcaaag tgctgggtct cgtcttgaac gtggtgaccg agggggcggt cagccaaacc
     9901 cttggcatgg tgcttggtgc gctgatcgtg ctcgtgtata cgacattcgg ggggatgttt
     9961 tccgtggcga ttctggattt cgtccagctt tccgtgatcg tgggtggatt actgtatatc
    10021 gccaatgtcc tcggagacct taccggtgga gtcggcgtgg tggtccgcca cgcgcagcaa
    10081 gcgggcaagc tcgagttctt cccgcccgct tctctgaatg catggattcc ctttgtgggc
    10141 gccggcgtga ccatgatgct gggctcgatc ccgcagcaag acgtgtttca gcgcattacg
    10201 agcgccaaga acgaacggac ggcggtccga gggtccctca tgggcgcggc gttgtacttt
    10261 gccttttgct tcgtcccgat gtatatcgct tattctgcct tgcttgttga tccgacaaag
    10321 tttggaacat tgctggaacg tgatttccag ctggtcctgc cgacgttaat cgtcgagcat
    10381 acgcccgtgt ttgcccaggt gatgtttttc ggagcggtgc tttcggcagt catgagttgt
    10441 tcgagtgcca ctctgctggc gccctcggtc atactgagcg aaaacgtgat cagaggcgcg
    10501 atgccgcgga tggacgagaa ggggctcctg accaccatgc ggatcgtgct cgtgtgcttc
    10561 gccttcgtgg tgttgaccat tgcgctcagt tcagattcca gcatctacaa attggtggtc
    10621 aacacctaca aggtgactct tgtggcggcc tttgttccac tctgtgccgg tctgttctgg
    10681 aaacgtgcca cgagcggagg cgcggtctgc gccatcgcag cgggtctggg gtgttggatg
    10741 ggttttgaac tgctgggacc tgcagcgtcc atctggcctc cacaactcgc aggattactg
    10801 atggccacgg tcgggatgat tgccggctca tacctcttcc ccgatggctc ctcgcgataa
    10861 acctgtgtga gccgatacgt ctcgtggcga tcaatcgctt tctcttgcgg tctcgcgtat
    10921 gcttgaccga atgccgtcat ggaatcgcat gttgcattca cgctggcttt tccgaggcgg
    10981 gctgattctg gccgtcagtt gtgtgctgct ggtggccggc ctgctggtct acggggcgta
    11041 tctcgccacc ttccttgagc ttcccagaaa cgaagatcat ccggcgctgc ggctatacac
    11101 ggctccgttt cagctcaaag ccggtctctc gctgaaggtc gcacgcctcc cggaacgatt
    11161 gcagcggctc ggctatcgag cggtgaaggc acccgttgct tcgcccggtg actatcaact
    11221 cacgccagag gcgctcacca tttttctgca cgcgcagcct gaaaccttcg tgaaggccaa
    11281 tgtggtcacc ctggcgttgg aagacggagt ggtcacacag gttctctcgg agcctgacag
    11341 aaccccgatc ttcccggtgt ttctcgagcc ggagctgatc agtggtttac gcggcgcgtc
    11401 gcgtcaagtg cgcgaatgga tccccttgtc ccagatgccc ccgcgcctgg ttgaagccat
    11461 tctggccgtg gaagaccggc ggttctattc gcaccccggg atcgatccgg tggcgatcgg
    11521 acgggcggtc tggcgaaacc tcaccaaagg cggagtggta caaggcggca gcacgatcac
    11581 gcagcagttg gcgaagaacc tcttttatac gcctcaacgg acatttgcac gcaaagtcaa
    11641 ggaagcgctt gctgccatcg tgctggaaac gaaataccgg aaacaggaca ttctcgaaag
    11701 ttatctcaat gagatctatc tcggccaagc cggtttcgta tccatctatg gagtcggaga
    11761 ggcggcgcat cgctacttcg gtaagaatgt aggcgagctc accacaggcg aaatggcaat
    11821 gatcgcggga ttggtcaaag gaccgaacac attcgcgccg acaaaacacc gtgccctggc
    11881 tcaacaacgt cgggacgtcg tcctgcgtct gttgcgcgaa caaggccatg tgacggagca
    11941 cgaatggaag gtcgccgtca atcaaccgct tcaggtggcg ccgcccgacg atgtcttggc
    12001 tgatgcgccg tattttctcg ataccgttct gcgccaggta gaggaagcga tcggcacgcc
    12061 gttgccggaa gggctccgaa tcgacagcac gctggatgca attgtgcagc agattgcgac
    12121 ggaaggcctc gagaacggat tgaccagact cgagcgggcc tatccagccc tcagaggatc
    12181 cgatcaactg ttgcagggag cggtggcgat actggagccc tccaccgggg cgatgttggc
    12241 gttagtgggc ggacgggact accgtgtcag tcagttcaat cgagcagtac aagctcaccg
    12301 ccaggccggc tcgctcttca aaccgttcgt ctaccttgcc gcgtttgaag ccagccgaac
    12361 tggcacgcca ccctatatga caccggccag cctgctggcg gacgaaccgg tgacttttga
    12421 atcggaaggt gtgacgtggg cgcctcagaa ctacgataag cagtttcgag ggaatgtgac
    12481 gctgcggagc gcgttggagc agtccctcaa cgtgccagca gtgcgtgtgg cgaaggcagt
    12541 cggcacgcaa tccatcctcg atgttatccg ccagttggga gtgacaagcg cgctgactac
    12601 taacgatctg tcgctggcct taggcagtcc cgcagtctcg ttgctcgaca tggtgacggc
    12661 atacggcgtg cttgccaatc gagggatttg ggtccatccc acaagtctgc gtgcgatatc
    12721 ggatcgagac gggctcgtcc tgtggacacc cgcaccagac cggcgccagg gcgtctctcc
    12781 ccaggcagcc tatctggtga cgtcgctgat ggagggtgtg gtgcagcgag ggacggctgc
    12841 gaaagcaaaa gtcttgggct tgaccggatc cattgccggc aagaccggga caaccgacgg
    12901 ctatcgtgac gcgtggtttg tcggatatac ctcggaactc gtcatcggcg tctgggtggg
    12961 attcgacgac gaacgagcca tccgtctgac cggtgctcaa gcggcacttc cgatatggat
    13021 ggacatcgcg aggcggatgc tgccggaaca cgtagatccg tttactccgc cctccggcat
    13081 tgttgcacga gccatcgatc ccaagaccgg acaattggcc acctcgcaat gcccggagca
    13141 ggttgaggaa gtcttcattg aaggaactga gccaaccgtg tattgcgaag tgcacggcgg
    13201 cggtatctgg gaacgtctcc ggcacaggtt tgggttttct taacgcctta ccgagcgcat
    13261 ttttacattg cgcctcagga acgtttccac attctcctag ggcttctggt tcgaatggtg
    13321 taagcttggc ggtgagctgg gaggaaccgc atgaaaaaga atggatttgg tggcgacgac
    13381 aacatcacgc tcttggcgaa gggcgtgcat ctcaagggag aaatcagggt ggaaggcacc
    13441 gtgcggattg acggccgtct tgacggagaa atacacacga aaggtcaagt catcgtaggt
    13501 gaagacggcg tcgtacaagg cctagttatg gccgggaccg ttgtgagcag tgggaggatc
    13561 aaggccaccg tcacggcagg cgagcgcgtg cagttattaa aaaccgccat cctgatcggc
    13621 gaagtgcatt cgccggtgct catgatggaa gaaggcgcca aaatgcaggg taccaccgac
    13681 atgggagtcg cggcctggcc tgacgacctg ccgaaattga ctggcacggt ccgtgagttg
    13741 gtgtcacacc gagccaagca cgcggtggtc gttgaacaag attccggttc ctagcccgct
    13801 gcgtctcgtc cccaacgttt gtctttcttt cccaggaccg caagcgtttg cggccgcatt
    13861 tttcgcccta ctcatttctt atggctgtcg gtcagaggcc ggcagatcac tcgatcggta
    13921 tcgtgattgc gatgccgccc atcacgtcat tgatcttgaa atcccgttta gggctcagcg
    13981 gatggctgtt atggcaattg atgcaggcgg cggagacggc gcggtccgga tagatggcct
    14041 ggaaatactg tttgcgtccg ctgctcacga tgcccgtgat cggtgcctcc ggggagcggt
    14101 tgaggaggtc cagccccttc cgctcaaaat cggtcgcggg ggcgttgcgt tgatagatcg
    14161 gtgagagccc gatgagacgg tagcggatcc cttgcccgct ttcggccacc aaccgcccgg
    14221 agtgttggag aaattgcgcg ggaagcggca gggcgttctc ctgctcccaa tgctcgacgg
    14281 ccttcacgac ccccttttct tggaggcgat tgacgatgtg cgtcgtgtaa atcgtccggt
    14341 gggcctggag cacggcatgc acgaaacccg caaccctttc cggggagatg ccgggcgttt
    14401 tggagtcgtc tttggcgggg taggcgctcg cgacccacca gaccgccagg attaggaacg
    14461 tgagacctgc ggtagagtac gcgattcgga agcggtccat gatgcctcct ttgatgaaga
    14521 ggcgggagcc atgagcggca gcgtgatcat gcaggacgta ccggccccct cgacactctc
    14581 caatcgaatg tcaccgccga atttccgtat gatgcggcga gccaccgtca gtccgagacc
    14641 ggagccttcg ccttgaccct tggtggtgaa aaatgggtca aaaatcttcg acagatggtt
    14701 cttgggaatg ccaggaccgg tatcggcgat agtggcgaca acggtttttt caccgacggt
    14761 cgtcgacagc cgcaacgttc cctggttttt catggcatga agggcattcg tcataatgtt
    14821 cgagaatgcc tgcttcagtt ggtccggcag agccatcacc accgtgtcgc cggcataggt
    14881 gcgctcgatc gtcaactgcg acgtgtccaa gccgaccatc actgccgcga gcgcttgatc
    14941 catctcacgc tccaaatgga ccgtctgacg ctgatccgat atttcgcggg ccgcaatccc
    15001 ggtaaaatct ctgatgattg ccgccatgcg cttgccgtgg tgaatgatgt cctgggcata
    15061 ggattttacc ctcgcgggat cgcactcatc ctgtagtgct tctcctaacc ccatgatgcc
    15121 gaacaagggg ttgttcaatt cgtgcccgat tcccgccgtg agaatgccca gacttcccga
    15181 cttttctgct tgtacaagct tttcctgcat gcggctttcg tccgtcgtgt ctcggagtac
    15241 gagccctacg cgatcggatt gtcccggtcg gccaatcaca cgaaaccatt cgtagcggta
    15301 caggtgagcg cccaattgca gttccgcctg tcggctccta tggacagtcg ccgtttctgg
    15361 aacgagcgga tctctgatgt gttctacggc ggccggtggt tcaccggcca agctaggggc
    15421 catgccggtc gtttggtgac tccacagttc ctggcgtaga tgctgctgtt gcgaaggctc
    15481 gaccggcaac atgtcgaaca gccgccgccc ggcagatgat tcctctgtca gggcaaaggc
    15541 ttcccgaccg gtccggttca cgtacaacac ctcttcctga tcgtccacga tgagaatcgg
    15601 ggtcggtact gcatcgagga tttggtcggt agatcgctgc aaatactgca cttcctgcgt
    15661 cttcagggtg acttgatcgg tcagaccggt gaacgccgct tccagttgcc gattcatgcg
    15721 gttgaattct tcagccaggg tttccaactc atcaccggtc ttgatctcga tcgactgccg
    15781 cagttctcca cggccgatcg attgagcggc ttgttgcagc tggcgaaccg gcgtgacgat
    15841 gcgcttggcg gccaaatagc cgagcgtcgc caggagtgcg acggcgatcg agccgaagac
    15901 cgtcatccac gtgaataaat gcttgatggg ggcgaaaagc tcatctgatg attgccagac
    15961 aaacgtatgc catgatccca cctccaacgc tccattcgta ttccggctcg tttccggaag
    16021 gggggaaaag ccgataatgg acgaggagcg tcctccatgg ccatcgcttg gtgcgctcgt
    16081 ccagccggac tgtcgctggg tgaccaaggg aatgatgctc gcgtcggaga gccggactcc
    16141 agtaggaagg atgggacagc tgacgacgat tcctcgatgg tcgatcagca tgacgtgccc
    16201 ggtctttccg aaccggatcg gatgagtgga tggaccaaaa aattccttgg catcgatcac
    16261 ccgatggatg acgccaatcg cttcatagcg gagactgtcc atgaccggaa ggctgatgga
    16321 gaacgcatac gtctgcgcca gctcgtcaaa atacacgtct tcgatataca gttgaccgat
    16381 gcctttgttg aacgtgccct tccaccaaag aaattctccg ttggcgaacg gcggcttgtc
    16441 agtgagcgcc gctgcgagat gcccttgggt atccgtcaca aacagcattt tcgtggcacc
    16501 acgcaccacc tgcggaatca attcatccgg ctcgctcctc gcacccgtat agtattctcg
    16561 caacagattg gcgacaggat tttccgtgat cattttcacg gtcgccggat ccttgctctc
    16621 ccacttaagc tgaaggtcct gcttgatggg gtagcgaccg gattccggct tcaggttcct
    16681 gtcacgtcgc tgctcgagtt cccggactat cattgggtcg gcggcgattc ttgaggtgcg
    16741 ggcgatctct tctcctacga gaaggtcgag ctttcttgcg gcttccgtgg ccagcgcttt
    16801 gaagctttct ccactgactt cgtgaatttc ctgagaccct tgccagaagg ccatgcccag
    16861 gccaacgagg agtggcacaa cacccaccaa gagcatagac acgatgagct tggtttgaag
    16921 cctccatcct cgcgtgacag gagagggttg cttggtcgcc ttcgtcacgt ccgttcctcc
    16981 ggaaccgcgg ggaaagtaat cgtaaacgtc gtccccttgg cgaggcggct ttccacatga
    17041 atcgtcccgc gcattttggt cacgatggtc ttgacgttat acagccctaa gccggtgccc
    17101 ttgccgggat ccttggtcgt gaaaaaggga tcaaaaatct gaccaagcag gtccggcgca
    17161 atcccgcacc ccgtatcagc aatgcgcagc ctcacgccgt catcggaggt gcgagtggtc
    17221 aggctcaacg tacctccgtc tttttccatg gcttgcacgg cgttggtaat cagattcacc
    17281 aacaggtgaa ggacctcatc cggactgcct cgtgcttcga ccccgtccgc gtactgcttg
    17341 tccacggtga tgtcgtgaaa actgaccgca tagcgggcaa ttttcaacgc ctcgtcaaga
    17401 cggttattga gcgagaccat cgtatcgtcg cgggccgacg tctttctggc atattgcgtc
    17461 aaatcccggc agatggcgct tgtccgccgg acggcttcga tgatttcgcg agcctgctcg
    17521 tggacgactt tgaggtcggt ttcttcaaaa agattttcgg ccaaccccat gatcagctgc
    17581 agcggattgt tgatgtcgtg cgcgatgccg gcggcaaaaa acccgatccc ggccaatttt
    17641 tctgcgtgga acagttcggc ttggagattg gcctcatggc tcaccagctc ccacaacaac
    17701 cgtgccgccg atccgctgag gacctgagtg tctggttgtt tatggagctg aataaactgt
    17761 tccatggcag ggtcgccggt gacatccatg atcaagttaa ccccgtggtt acccacgaga
    17821 tcttcgatcc ggtcggtcac agcaatgtga aggtttctgg cccgcaaaag accgggtgcg
    17881 gcagggtcgc gatctgaaat ccctacgatt tcaatcgacg gaatgagatg caataaatca
    17941 aggagagcgg ttcccccacg cccgcctccg agaatcgcta ctttggtggg cgcaagcttt
    18001 gcggccaccg ttctgtcagt cacaggctgc acgtcatggc tccggggatg gaaggggagg
    18061 gccagccctc cccgacatca taccccatgc acaagcctag aggtgcgcga gctctcgttg
    18121 ttccatggcg cgagcgaccg actctctgag cttctcgcct tccaccggct tgacgagata
    18181 gtcgacgacc ccctgccgaa gcatcgacgt ggccatgtcc gcatcaggaa atccggtcag
    18241 tacgatcacg ggcactctgg gatagttggc cctgaagtag gcgatggctt ccacgccatt
    18301 gatctttggc atccgaatat cgcaaatcag gatgtccaac aacagcctgt tctcgcccga
    18361 gttgatggtc tcgatggccc gttcaccatt ctcggcttcg aggacatcat agccggcttt
    18421 ttggagcgtc atgcggacga ccttgcgaat gtccgcttcg tcatccacga ccagtatgcg
    18481 cccatttgtc gcttgatcgc ccatgaaaaa tcccgatgta aattcaccca tgatgtcctc
    18541 cttggtgaac cgatgaagtg atgtttttcc gcatgccgcc gatgcgcggt gatagagcaa
    18601 gcgacatgcc ttgatgtaga ccgacgtctt tcgtgacata tcaggcattt attcacgatg
    18661 atcatggagg tgttcactcg agatcggtgg taatccacca ccgcaggtcg aaatccacca
    18721 ccaatccacc accacctgct gcggctggtg cagcgtccag gcagatacgt cgcagcagca
    18781 agcgagaact tatcaccact gtggttgccg gtggcatcat cgtgaatggc atggcccgcg
    18841 tccttgcctc tcaatagact tcacaccaag ttctgtacca aagttccgga acacccacat
    18901 aatggggcat gtccttcgtt tctcaacgtt tcgtcatttg gacgctgagt cgtgctggat
    18961 ggagaagcga ttccaattgg aatcactatt gattcagtga atccaaacgc attgttttga
    19021 taggcccgaa gtcgaattgc gtttcccaaa gacgctacgg tatcctgaca gcaggaaaca
    19081 cgagcacaca cctcagccct atcggggaag acgaggagtg ctgtctaact gacgggaaga
    19141 gggtcattga ttaccgaaca gacactgacg gagtacatca ggcgcgccat gccggatgcg
    19201 gccgtcacca tgatcgataa gaccggcacg atggatcatt tgaaagtcat gatcgtatca
    19261 gatgccttcc aggggaagaa cctgctcgat cgtcatcgcc tgatctatca agcgcttgac
    19321 gctccgatga aggacggccg aatccacgcc ctggagctca ccgccaacac caaagatgaa
    19381 catgaacagc gggtaactgg tacacgctga ttgactgctg gaaggagacc aacatgggag
    19441 atccaatcga ggaggagatt cagaaagaag tgaaaggcca caagattgtg gtctacggca
    19501 agggcacgaa gcaaatgccg atgtgcggtt ttacacgcga aacgatgcag tttttcgaca
    19561 agtacgggta tccgtatgaa gtgatcgatg tactctccca accggccaag cgggaagccc
    19621 tcacgaaaat gaccaattgg ccgaccctgc cgaaagtctt tatcgacggc accttctatg
    19681 gagataccga cattttggac cccatggcgg cgaaaggcga aatagagccg ctgttgaaga
    19741 aagcgttcgg caagtaaggt ctgccgcagt ctcaccgctc gaacttccgg ggggacctag
    19801 caaggcctcg tccgtggcag gctgtattcc tcgcgacgac tcacacatca caagcctcgc
    19861 cgttgccaat ctatgagcga gatttagcaa cggccgtgag cgccggtgag tgtgtgctgc
    19921 ctctcttcgg cgaagacgac tatcgcttcg ctgtctgcga acagcagttc cgcggatggg
    19981 aaatggacgg cagcaaatcc gtgtgatgcg aacggcgaat cgcgccgatc acctagtctg
    20041 tgaggctgca gctttcgcct tcggcttgtc cgcttctcgt gacagtcctg tggcgatgcc
    20101 gacgagggca aaggccctct cggaaggact ggacagagtc ctcgcccacc gcacggtgat
    20161 gagtttatag ccgctttgtg catgcacggc gctcagtgct tcaatgagct tgaggcgttg
    20221 agccggttca ttcgactcac gagccagagc tatcgccgcg ctgaggtcgc ctttccgcgc
    20281 gagtgctttg gccatggctt cgagagcccg actgatatca agctccattt tcacaagctc
    20341 tcgccgtcga ctgtcatcgg cagtcgtctg gctgcccagc cgaattttct cctcgacctg
    20401 ccggagctct ttcctccttg cacttcgctg tgcttccgtt tctgcgaggg cgagaaccgc
    20461 gtgagcctcc ttgatgtgtc ccaagtctgc ctgggccatg gcaatcccgc tgtacagcca
    20521 tgcacggctg acatcggtgt cttgaaagtg agcagcggtt tctctggctt ccttgccctt
    20581 gccggtcttg accatctcca cgagaatatc tgaagcgact gtttcataga cggttctccc
    20641 aagatcgtct gcttgaggcg cccttttcac catagcggcc gtgttgaatg cacccgctat
    20701 atcgcctcgc ttggcctggg cctttgcaat gtcatgaagg gcagagttgc gatgtccatc
    20761 cttctcgatt gatttggcaa tgttgactgc agtagccggg tcaccgacta tcgccgtttc
    20821 ggccgcgata cgccggagga tatcggcacg ggtccaaacg ttgacggtcg aacgggctga
    20881 cgtgtcagct tcctggagaa gaagcagggc caactggttg gccccatgct ggcgatggta
    20941 ttgagcgagc tccaataatg cactggcctt ggcatgcccg ggtttgatcc ccgaggccgt
    21001 cacctgggca tcggtcagcc gaccttgtgt gatttgagct tgcacgatgc cccactgtgc
    21061 atagcgttga gacgcatcat cgacgcgtcc aacaatctcc tgcgcctcgc tgagtttccc
    21121 attccgtgct cgatcgccaa cgatcttcat caacggatag ggattctctc cggctatgcc
    21181 cgagtaagtc cgcaacgccc cgggaatatc atcttgcttg gcctgggcct caacaaggtt
    21241 cgcagcgacc accccgacga tggcaggggg ctcctgtttg gtacgttcct tgaggcgctc
    21301 ataaatacga aaagcttcct cgatgttccc gctcttcaca taagccagag ccagttgtcc
    21361 aagggcattc gaggcagcca gtttgccgat ccttgaggac tggtcttcca cgacgatttc
    21421 ggcatacaga cgttccgtct ctggaatgtt tccggccttt gcaaacgctg cggcgagatc
    21481 ggcgaggtag gtgagcttgt tcggcagctt tggaaacatc ttgagcgtct cacgcgctcc
    21541 agacggatcg agctgaactt gtttgccaac aatgacggtg agcgcgtcag tccgttccgc
    21601 agtctcctga atggatagag cagcattccg cgcctcgagc agaagtgccg ccgggctctc
    21661 tgattgacta ggatgggcct ggcctgcgga cccatggcag ggaaagaggc ccaacagcag
    21721 cacagcgaac accgattctg caggcaaacg cagtgcggta tagccagtca tcgtttggct
    21781 tcaggaggag gccaattcat ttccgtgtgg cgaccacagt aatagcacaa cagtcgatac
    21841 cgcgccttgc gtcgaggatt gggcattgag ataaatgtga tcggggtatc gtcgaacgga
    21901 cacgttggtt ggtcatgcag cagatggact tcggccatca tggtaattgt tgccgtatcc
    21961 cagggtggcg atgtctcttc tttcccggcg atctgctctt tctggtgaca ccgttcgcaa
    22021 acggcttcgt acgtcttgac gcgcgcgtga tgcggtgtca ggtcggtgat ttccatcggg
    22081 ccgccacatg ctccattggt gcaggtcaga tgctcatgtt gcagcgcctg caagaatcta
    22141 tggtgagcct gccggtcttg ctcttttttc atcctctgtt tccctccttt tagtcgtttt
    22201 tagggcagcc tgtactgtcc ttgactgcgc gcgtgaacga gcacattctg atcgtgcgcg
    22261 ttccgcgagc aagaagggca gtcaggctgc actctcatat tccccacgca ccgctgatgt
    22321 gaatgttggc gccgttcaca taccgggcct caggactcaa gaggtagcgt acagcagcga
    22381 ccgtatcctc gacgctgccg atgtacccgg cgggaatacg tttggccatg ccggtaagtt
    22441 ctccgggagg cgcgctcccg gaatcgataa accccggcga gattgcgttc acggtgatgc
    22501 catgaggtgc cagtagcttg gcgagcgtgc gtgtcagaat cagcacaccg gcctttgcga
    22561 tatagtgagc ggtcacatct ggttggccga tcatgtgatc ggcattggcc atgctgaaat
    22621 tgatgatacg gccgaaccga cgagccttca ttccaggtgc ggctgcctgc gcgagataaa
    22681 agatggggtg caagttgcca tcaaacatct cattccagcc ggtgacggtt tcctcaaaca
    22741 gattgacgcg atgatatggt cccgcgccgt tgattaatgc atcgatgtga ccccattgct
    22801 tttcaaccag ggcgaccatt tctttggccg cgacggggtc tgagacgtca cagcggatcg
    22861 ccgaggcctg gccgcctgcc cggatgatag cagcggccgt gtcgttagcc tcggcttcac
    22921 tggttcgata gcacaacgca atgtgccatt gctgtgaggc caaatccaaa gcaatcccgc
    22981 gtccgatgcc tttagcaccg ccagtaatca atgcgacctt catcgtcata ggtcatccct
    23041 tttcacgttc ttgagcgcag tcgtttggca atgagttgta aggctccggc tgtccggttg
    23101 gaaaacgatt gttcctgttt cggctttcca gcgtcaagga tgcttgcgtc gatcagcaag
    23161 cgcagatcat cgatggtatc gacatcgcgc cattcaggaa gcaacgcgac ccgaagtccc
    23221 atggcgatcg ccttttcacg tgtcattgag agcacgcggt cagtcgacca ggggatgccc
    23281 tgaaacagtg ccggcgcgat tttcttgagt ccaatcatgt agtagccgcc gtcgagggcc
    23341 gggcctacga ccacatcgtg gcgctccaat tgatcgagag cctgccggta atagtcgaga
    23401 ggaagcgatg gcacatctgt gccgacgatc aagacctgct gataattccg ctgaaacagc
    23461 gtctcgcagg cgcgatgcat ccgggcgccc aagtcttctc cttcttgctc gagtaagccc
    23521 accgcttgac gctcctccaa gatccggaag aatgccaatg ccgacgatgg cgcacaggcc
    23581 aggtaccgat cggcttgaag cttcaacctc gacacggcgg ctttcgttct ttcgagcgta
    23641 tcgagcacga aggtcccatg aatggttgcc gcttcatcag ccgtgagcgg aggacacagg
    23701 cgagtcttga cctggccggg caccggcgcc ttagcgaaga tgaccaatgc cgtgcgggcc
    23761 ggttgggggt gagtgggcat gggcttcatg gtaccgttgc tcatctcaca tcggcataga
    23821 gtctcgccag tcggtgggga ctgatgccga tccaatacag gaagcgcaac gcccacatca
    23881 agaggatcgt tcgcaaaggg ccgcgccgct cccaccgacg gaacgaggtc gtgacgaaac
    23941 ctctgagggc gaccgtgcga ccggtccgtt tcagacgggc gctcaactct atatcttcca
    24001 tgatcggaat gccgggaaag ccgccgagtt gctggaaaac ctgacggcga acgaacatgg
    24061 cttggtcgcc cgtcgagatg cgactgagcc gggaacgcag gttcatgaat aggctgatga
    24121 tacgacccca catcgacgga cggtcgaatc ggaggtcgaa ccgtcccccc acgacttccg
    24181 gatcggccag cgcgaattgg atgagccgtt tggcatcaac cggcagatgg ctgtctgcgt
    24241 gaagaaacag caagacgtcc tgccggcagt cccgcgcgcc ggcattcaac tgcatcgacc
    24301 tccctgcggg agcggcgaga cggcgaacgg gcgttgagcc tgcgtttggg ctgccggcga
    24361 ccatgtcgac aacgacgttg gtccgatcgg tgctcccgcc gtctacaacg agaatctctt
    24421 cgaagccaag ctgtcttgtc agttggaggg tcgtttcaat gaccgaagct tcgttgaggg
    24481 tcgggatgat cacgccgacc gtcaccgcgg cacgacgatg agcaaggtgg gcgggttccc
    24541 atggggatga ggaggacgtt cggagtgtgg acatcgcctc caggccggtc gatcgtctta
    24601 gcccggcttc tttggttcgt tgaacatgaa gtacatgacg atgatcacgg tggcccagaa
    24661 gaccacttgc ttatgccaga acagcacctc gccatagtga aaaaatccca gggccaggcc
    24721 gatggcagcc gccatgacgc cgtaccgcac ggcgggcatt tcgatcgcgg cattggccca
    24781 cacggcgaga ccgccgaaat aaatgagaaa cgggacgacg ttgatctcga atccaccgct
    24841 aatcgacaga aacacattga gaaatccgat gaacatcagt gccgttccga ccatcaggcc
    24901 gatgacgcgc atttgatgat ccccgctgct ctcttggctg tctgacggtg actgcggtgg
    24961 cttgggggaa gagcgttgcg ggtcttgggg agtggggaga tcggtcggtg ccatgggggt
    25021 cccgtgagtc gccggtcaac cggccattcg ccagtggcgc agccaggggg tgcgccgcac
    25081 cccggatcgc tgagcctcga agacagagtg cttcatgcct tgaaatgctt gctgagagtc
    25141 aaggcttgtt gttgatagga agagccgaga ccgacgccgt agagccgttc aggcacgtcg
    25201 agcatcctat catagacgac tttaaaaaac gtctggccgt catggatgag gaacgggacg
    25261 tcgtgcgggc gtacctcgag caccacctgg gtgcccttca attctccgtc ggctccgtag
    25321 ccgaatcccg gatcgaagaa tccggcgtaa tgcgtccgga gttcgccgca ggccgcttcg
    25381 taggccacca tctcagcggc gtaatccggc ggcacacgga tccgttcttt cgacgccagg
    25441 atgtaaaact cttctggttc gaggagcaga ctgtcctgcc gatgccggta gagcggttcc
    25501 caaaaatctg tggcggcata gtatccgatt ttggagaggt cgatcacatg actgtttttc
    25561 ttggctcggt acccgatgac tttcgaatcg gtgcgctcgt cgcccttgag gtcgatgcgc
    25621 aggaaaagac cgcgatccgt acggaattcc ctttggcgaa gaggcttgcg gtccgacagg
    25681 ttgtggtaga ggaggggttc ttgacgatgc agatgttgca gggcggcatc tgaggcggct
    25741 gcttcgccgc gcacaaatcg gatttggttg agggattgac ccgtcttgac cttgatggca
    25801 aaggaacgcg ggaccacctc gagaaacaac ggcccttgat acccggcgcg aatctcgtca
    25861 aacccagcat tcaagtcggt gacaattcgg gtgaacacgt ccagccgtcc ggttgtgctt
    25921 tttgggttgg cgcgggcacg gagtgtggag gggagacgca gcgactccaa gagcgggacc
    25981 agatagacgt ggcccttttc gagaatcgcg ccgccagaca ggtccatctc gtacatcact
    26041 aagtccgact ggtaaaaatc catgacgtcg agacggctgg agatggccga cagttccggc
    26101 aggaaactgc tgatcaatcg ataggccttt ttccccaggc ggaggtccag actggccggc
    26161 tgaatttggc ggtcctcgat gggtgtgggc gcgctgatgg tccctgcggt gatgagtcgg
    26221 gtgatttgct ggtagggcag aatgccttga gcaggtcttc ttgtcacagg tcgtcgatct
    26281 ccccgccgca gctgttgccc caggacgaat aaccgtcccg ctcaggatag ctgtcggcgc
    26341 acgacgaatc gtcataggac gtccgtgcgc caaccatcgg caattccctc ccattaccgg
    26401 ctttcggctg cggataatgg aaggtcttgt tttcataatt tttcaggatt gcaccgtcct
    26461 catccatatg cacgatatag tcgtcaggaa gcagggggat tttgcctccg ccgcccggtg
    26521 cgtcgatgac gaaatgaggg acggccatgc cgctcgtgtg accctgcaac gctttgatga
    26581 tgttgaggcc ggattcaatc gtggttcgga aatggttggt gcctttggtc aaatcagctt
    26641 ggtacaggta atagggtttg acgcgagcca gcaacagttg gtgcaccaat cgcttcatcg
    26701 tttcgggatc gtcgttgacc cccttcaaca acacggtctg ggcacctaac gggacgccgg
    26761 catcggctaa catgccgcaa gcctccttga cggccggtgt cagttcgtcc ggatgattga
    26821 aatgcaggtt catatacacc ggatgatact tcttcacaat gctgcaaagt ttgggggtaa
    26881 tacgttcggg caacgtgccg gggacgcggg agcccagccg gatcacttcg agatgagtga
    26941 tggtcctgag cgctttcaag atgcgctcca ggagatggtc gggaagcagc aagggatcgc
    27001 cgcctgacag gatgacatcg cggacttccg tatgcgcctg caggtaggca atcgcttgat
    27061 cgagttcacc ttttttgagg aacccgggtt tccccaccag gcgctttctg gtgcagaagc
    27121 ggcaatagat ggggcattga ttggtcacca tgagcaacac gcgatccgga taacggtgaa
    27181 ccaagtgcgg gacaggactc atcaggtctt cttccagcgg atcgtcttcg gcgtcgatgt
    27241 cagtcagttc ggcgggatcc ggcatcacct gcttccaaat cggatcaccc ttctctttga
    27301 tcgtgccgag gaccgtcggc gtgatccgca tgggatacgg accgacgatg gcttcgattt
    27361 cctgttcatc catcccgaag cgctcagcca agtctttcgg cttcacgatg ctgtcggcga
    27421 gaatgcgtct ccagtcttcc atccaataca ctccttcaat aagcacacag tggaatggcg
    27481 gcaggcgcac accttgactg cttttgctca ggtccctgga gaagtggtga gataccagga
    27541 gacttcggtt agccgttagc cagacgacgc gattccgcct tgttctccaa atgggccaga
    27601 atttcgcctg tgtcgctcaa tacggtctcg ccatcctgta ggaccggtac ataatactgc
    27661 ccagagacgg caaagacttc tttacgttgc ggccggaaat ccgggacaat cacgtcctcg
    27721 tagggcaggt ttaattcggc gagctttcgc cgcaccacgt cacactcggg gcaccactgc
    27781 acatgataca gggtcatcgt cattcggtcg ctttcccttg aactgccgcg caggggacca
    27841 tgatcgcatc cttcatgcca tggatcactt ttttatccgc gggacagacc gtggccagaa
    27901 taccagccaa gtcgactttt tcgccagtga cgaagtagta cggagacagc ctcgcgcgac
    27961 cggccatcgc atggatgtcg gccgtcaccg ggtcaaaata ctccaagtga tactgccgtc
    28021 ccttgtgaaa ctcctgcaag acatacgccg tgtgtgggaa ggagcggaga gctgaatcga
    28081 tcgcagctgt ccattcgctc tgggggagat cgtggccgac cgagacgcct cggctccccc
    28141 aagccaactc cgaaaatccg gacggcttaa tgacaagatg tcgttctttt tgtgtggctt
    28201 cggccaaatc gctccagttc gtgactgcac ggccgcccac cgacaggccc ggaattacgg
    28261 cagtaggcgg cacaggagcc ggatccagca cccacgtgcg aggcatgacc gttgagagat
    28321 gtacgtacac ctcctcccct aactctctcc gccagaaggg gaccaacgtg ggatggtgca
    28381 agagcccaaa ggccgctttt tcttccaggg ccggcttgta tggaggcgta atagacaccc
    28441 aacttttctt ggccgcgtat tgaatcaact cgtttttagg aatgttcggc aggtcgaaga
    28501 gttcgtagaa gcgatagatg aggccgatcg ggcggtcgcc gccttccgtc acaaggaaca
    28561 gcttcccttc ggtaaagcga atgtcgcgcg gttggatgca ataggtttca agccccccag
    28621 ccgtcagttc cttggcgagc cagcccatct cgggccggta atccttcgcc tcgtcagaca
    28681 cgacgatggc gacgacgcct tcctgctccc cccggtagga gcggagcatt ccggcaaatc
    28741 cattgagcat tccgtcaggg ccaccgacga gttgcgcgcc agctgtggcg ttgtcactct
    28801 cggatgaggc gggctggctc tcatagagac gcgacataca ggcggtcagc ccgatgccac
    28861 cagggacgga atcaagctcc gtgatgacca tcccatcctg cgtcggaatg acgtcaggcc
    28921 gaatgacccc gggtagctcg tcgcggaagc gtttcatccg gctgtatctg atcagcgctt
    28981 cgggtttccc ctggtcgagc cagtccgcga tccagcccgg ctgcgtcgtc cctttagcgc
    29041 tttccgtata caggcggttc aaggccttgt agaaagacag gagctgcggt ccgagcgcct
    29101 caaaaaatac catctgctca cgggtgagcg gcaaaggttc aaggccgatg cgccaagagg
    29161 cagaagccga atcttcgtcc ggtgccgtca gggaagagct gacaggttga ggcgcgaaaa
    29221 gacctgcttc aatcagggca tcacggatac tggagcagcg gtaaagaagg tcagaggttg
    29281 ccaaatcaga cagggaatcc gttcgcgaca tggatcagct gcgagaatcg tatcacgcga
    29341 ctttacgaac ctacaagggg agcgaatagc ggcgcggcgg gattcgcggg agaggccaat
    29401 cgcacgcagt tcttaccggc cgtcttcgcc gcgtagaccg cggcatcggc ggccacgacg
    29461 agatctgatg gaatagacgc atggatcgga aacgtcgcga cgccgatgct gcacgtcagg
    29521 gcaagcggat gtgtgttgag tcccgtcagc tccggaagca tggtgagcgt ggtgacgcgg
    29581 tggcggagac gttcggcggc actgagcgcc ccatccattg aggtatccgg gagaatcagc
    29641 gcaaactctt ctccgccgta gcgcgcggag aaatcgctcg accgggctgt gccccgtcca
    29701 ggcgcctgtt ggctatccaa taaccagccg acttcccgca ggacttgatc gcccatcgta
    29761 tggccgtgtg catcattgat ctgtttgaaa ttgtcgagat cgatgaagag cagcgaaaga
    29821 ggacgcattg agcgaagtgc cagcgagcag aggtggacta aggtttcttg aaagtgcgaa
    29881 tggttgtaga gtcccgtcaa actgtcgcga atggattggt cacgcaagag ctgattggct
    29941 tgcatgaggt ctcgattcgt gtttgagagt cttttgacca gaccttccgc ttccgcctgc
    30001 tggcgcttgg tcgcggtgat gtccgtgatc gtttcgacga tatgagtctt tccgttcgcc
    30061 gtctggacct gcgcccattg gacgagcagg tactcgtcat tgggaagcgg cacctcgctg
    30121 gcggtaggct ttccggtatc gactgcttcc ggcatcttgc agaacgcaca cggcgtgggg
    30181 caattcgcta tcttttcatg gcaggtcaga ttcgaaatat cgccgaactt gttctgggaa
    30241 gtctgatttt gaaacacgac tctatgagtg agagggtcaa tgacggtaat gatcaccggt
    30301 tgcgagttga gcaacacttg gggatcatat tggggccctt ctggggggag cgccatcgtt
    30361 gtgtacgttg gaggaacaat actggaatca gtatttcagt cagggctgtt ccggtcaaga
    30421 atttgagtgt tttacgaatg gtgatagaat gtgctctttc ggtgaaagct ctatggcggc
    30481 actgcacgcc aagaatgtga tggctcttac aggggcggcc cgcggcttgc ctcaagcggt
    30541 accgaatagc gcctgcttcc attgcgacgt ctgctgccgg tttcccgatc cggacagcgt
    30601 gctgcggccc tatttcacag atgaggaaat cgccagcgct gtcgatcgtg gtctgaaggc
    30661 cagcgcattt cccgacctga acggctctca ggtcacattg gtgccgaatc ctcagggaga
    30721 aggatttctc tgccccgcct ttgagtccac gaccggcagg tgcctcatct atgaccagcg
    30781 gccgctcgat tgccgactgt atccgttggt gctcatgtgg aatgccagcc atgaccaggt
    30841 agtgctgggg tgggacagca cctgtcccta caccaaggag cacgtgcctg attccatccg
    30901 ccgacatgcc gatcaggtcc tggcggttct cgaagagcag tccacggtgc agcggatcgc
    30961 cgaccatccg aggctggtgg ggcggttcca atcggaggcg ctggtgctgg ctcctcttcc
    31021 gggccttaca cgggcgatct ctacgcgatg gggaccggaa cagtgccggc ggctcgtcct
    31081 tgaggatctc tcacgcctca agaccgccct cgaccgatcc aaaatatgcg ggtcacttgc
    31141 cgcctattcc gctccgtatc attatatgtg gaacgcgtta cttccctact ggtggatgga
    31201 cctggagggg gccctatgcc tcttcatcca atcgcctgac ggatggttca tgccgctgcc
    31261 gccactggct gagggcgctc tgacgcggcc gttgaacaag gctttcgatc tcatgcggca
    31321 gtggaacggg gccagcgccg tcagccgggt cgaaaatgtt cctgcagtgc tcgtaccgac
    31381 gctcgtcgag atggggtatc gcgtgctgcc gaaaagtccg gactatgtgt atcgggcgga
    31441 cgatctcgcc aggctggcgg gcgatcggta caagtcacag cgggccctct gtaatcgtgt
    31501 cgagcgggaa ggtggtatgg ttgtcgagct ctttcgtcct caggatcggg cagcgtgccg
    31561 tgcgttgtta cacaaatggc gaagccaaaa actcgctcga ggagcggacc cgttggctga
    31621 actgctgctc gaggattcgg tctctgccca tgaagtcgct tggtcccatg cgtctgagtt
    31681 gcaactcaca ggatcggtac tgcgaaggaa tggcacgctg tgcggctaca cgtttggcta
    31741 ttggctggat cgccaaacat ggtgcgtgct gttggaagtg accgaccgga cgataccggg
    31801 atcggcgcaa ttcctgtttc gcgagacatg ccgtcacgcc cgggctgagg gcgccgaatt
    31861 tatcaacacc atggatgatt caggattgac cggtttgcgt ctctcgaagg aagcctatca
    31921 tccggccgcc cgcatcgaca gtttcgtgtg ctctgagatc ttgcagccat gagccagcag
    31981 gattttcaac aacccctcga ccccccgcgg tatcgctggc tgccccacct cattatcctg
    32041 atgaacctgg tcgccgtcgc tgccggtgtg tttttgcttc gcagcgtcga acagcggctg
    32101 gtgtactccg ccggggaaga attgaggatc gcggcagcgg aggtggcgga taaactggac
    32161 cggctcctgt tcgaacgata cggcgatgcg cacatgatgg ctcaagccct ggcgttgcgg
    32221 ccatcggaca ccgcctatct tacgagctac gtcggttgga tgaaaacgaa tcatgcaccg
    32281 gtgtatgcat ggcttggggt cacggatagt cgtggagtga tgatcgccgc cacggaatcc
    32341 tccctgcgcg gccaggattt cagtcggaca ggctggttcc aacgcgtcag ccaaagcaag
    32401 accattcagg tggacgacgt cgcgttgcat gaaggcgaag gggaaacgga aagcgtcgcc
    32461 ttctccgcgc cgatgcttga cgctgagggc aatctgtccg ggatcgtcag cagtcgagtg
    32521 cctgtatcgg tattggagga ggtgacgaca gggacgttac gttcgctgga agcgcggccg
    32581 gagttcgcgg gagccgtcgt gtttcaaatg gtgactgacc aagggcgcgt atttgtggat
    32641 tcggaccttc gacggaatcg ggccttcagt cagcgggatt caggcgcgtt gtcttccagg
    32701 gcggcaccat tgggcgatct cgggtttgtc gaagaacagt cccatggaca agctatcgtg
    32761 acggggttcg cccggacgaa aggatttggt cagtttccag gaatgggatg gcatgtcctg
    32821 gtgcgcatgg atcgcgatcg catcctcgag ccgatccttt ccatattatg gaaggtgagt
    32881 ttgatgggcg cggtcgtgtg ggttcccatg gtcgtccttt tgttctggtc gacctcacat
    32941 ctccgggcgg aatacagaca ggtgcaacag gaaagctcct gggcacgggc agcggaagcc
    33001 gcgctacttc agagccagga gcgcacaaga gccatcgtgg aaacggcgct ggatgcgctg
    33061 gtcacgaccg acgcagcggg tatcgtgagt gattggaacg ggcaggcttc cgcgatattc
    33121 ggatggtcgc gcgaagaggc gttgggcaaa cgcctcacgg aactcatcat cccgcaacgc
    33181 gaccgtgagc atcacgatcg cggcatccgc caatacctgg ccagcggaga aacgcacatc
    33241 ctcaaccggc ggattgaagt gcaggccctg cgccgttccg gacaggaatt tccagttgaa
    33301 ctcgcgattt caccggttcg cgtgggagac acgatatttt tcagcgcctt cattcgggac
    33361 atcacggagc gccgggaggc ggacacgaaa ctgaaagaga gcgaagtccg gtaccgatcg
    33421 gtggtgaacg cgctcgatga aggcgtcgtg gtcatcgatg catcagggtt cttacgcacc
    33481 ggcaacgcca gcgccgagcg gatcctcggt gtgtccggcg aggagctcac cggccgtacg
    33541 ttgcgcgata agcagtggcg aaccattcgt gaagacggat cgccgttccc acccgagcaa
    33601 tatccggcgg cgatcacact ggacacgggt gagccctgcg ccggcgttgt catgggaatc
    33661 taccgaccgg atggacagct gcgttgggtc gagatcaact cccgaccctt gaccccggat
    33721 ggcggagcca gccccgccgc cgtggtgatc tcgttttcgg acatcaccga acggaaacgt
    33781 gtggaggagc ggctcaccgc ccagtatgcg gtcacgcgcg tgctggcaga atcgcgaagc
    33841 ctggaagatg ccgttccgaa gatcatgcgg gccgtcggcc aaaatctcga gtgggattgc
    33901 ggcatttttt ggcgcgtgga tcgcaccacg tccgtcctcc gttgtctgga tcaatgggca
    33961 ggggccgggg ttgaggcgca gtcgtttctc gagaagacct gggacctgac cttcaagcag
    34021 gaagaaggct tgcctggtcg gatctggtcg agcggcaagg ccgcgtggat taccgatgtc
    34081 gcgcgcgatg ccaatttccc gcgacgactg ctcgccaacg aggtcggatt ccacggtgcg
    34141 ttcggatttc ccgtgcgggt gggcggcgac attgaaggcg tcattgaact gtacagtcgt
    34201 caggtccgcg tgccggataa cgagctgctt aagatgatcg acgacgtcgg cctaaaactg
    34261 gaacagttcg gtgatcggac gcgagcgcaa gaagccctcc aacagacgga agcacagctg
    34321 cggcaatccc agaaaatgga ggcagtagga cgcctcgcgg gcggggtcgc acacgacttc
    34381 aacaatctgt tgaccgtcat ccggggatat agcgaattgc tgctcgcacg tctgccggcg
    34441 gatgatccga ttcgcaaaga tatgactgag attaaaaaag cggctgaccg ggcctccggg
    34501 ctgactcgtc aactgctggc cttcagccgc cgtcaattta tcgcgccgaa agtcatcgat
    34561 ctcaatgccc tcattgcaaa catggacggc atgttgcgca ggctgttggg ggaggacatc
    34621 gtcgagctct gcactgaact caatcctcac acgggatgga tccgagcgga tgccggacag
    34681 gttgagcaag tgatcatgaa ccttgccgtc aatgctcgcg atgccatgcc aaaagggggt
    34741 cgtctcacca ttgagacccg aaacgtgacg atcggccaac cgacccgtca aacgccggcc
    34801 ggtgtgaagc caggagcata cgtgctgttg gccgtacgcg ataccggtca cggaatggat
    34861 gaagaaacac agtcccatct gtttgagccg tttttcacga caaaggagaa gggaaaaggc
    34921 accggcctcg gactgtcaac cgtgtatgga atcgtgaaac agaatggcgg gagcatctta
    34981 gtcgaaagcc agaaagacca aggcgcgacc ttcaagattt attttcccct cgtcgagcag
    35041 ggtgcgccag gcatggtcgc gcctgacgcc gaacctgtgc atggccgcga gaccatcctg
    35101 ctggtggaag acgaaccgtc ggtccgtggt ctggttcacg agacgctgcg actccatggc
    35161 tacaccgtgc tcgaagcccg tcatggcatc gaggccctca tgaccggtgc caagcatcaa
    35221 ggaccgattc atctcttgct gacggatgtc gtgatgccgc aaatgagcgg cccggaagtc
    35281 gccgaaaaga tccaaattgt gcggcccggc atcaaggtgt tgtacatgtc tggctatccg
    35341 gatcatccgg tgtttgagca agggggcatc tcgcgcgacg cttcattcct gcccaagcca
    35401 ttcagcccga atgtgctggc aaagaaagtt cgtgatgtgt tggacggcgg aaaggttgca
    35461 tgacgggagg cggaaccaac gcggggcatt tgcaccggta gagtgttcag agttttgcct
    35521 gtcatagagt tcgtcgactg cctcttttcg tcggccaaaa gatttcgggg accccttgct
    35581 tgatcttctc accgggttcc gtttatcgtg cggtgcattc atcgattcga gaacgcttct
    35641 atgacgcagc aaggacgcga gttcgacatg tcgcagtatc gcatcgagaa agaaccctat
    35701 tacgccgacg ttcggggtga agtggatctc tttcttactg cggccaagag ccggatgccg
    35761 gtgatgctca agggacccac cgggtgcggg aaaacgcgct tcgtacaata catggcgtat
    35821 cgcctcggcc ggcccctgat caccgtggcc tgccacgaag acttgacggc gtcggacctc
    35881 gtcggtcggt acttgctcaa aggtcaggaa acggtctggg tcgacggacc actgacattg
    35941 ggtgtaaagc agggcgcgat tgtctacctc gatgaggtgg tcgaggcgcg gaaggatacg
    36001 acggtcatta ttcatccgct cagcgacgat cgccggctcc tgccgatcga gaaaaaaggc
    36061 caggccatcg aagccgcaga aaatttcctc ctcgtgattt cctacaatcc cggctatcag
    36121 aatgtgctca aggatttgaa gcctagcacc aaacagcgat ttgtcgcgat cgagtttgac
    36181 taccccactg ccgacatcga agcctcaatc gtagaacgtg aggccggcat cgctcacgac
    36241 cttgcggtca ggctcgttaa gctgggcggg aaaatacgcc atttaaagaa tcacggcctc
    36301 gaggaaggcg tgagtacgcg actcctcatt tacgccggga cgctcatccg cgagggtatt
    36361 gcaatcgagc gggcctgcga cgtggcgatt gccagaccca ttaccgacga tgccgacatg
    36421 cagcgcagca tcatggaact cgtcaaggcc atgttctgaa cggtatgcga atgtgttgcc
    36481 cgcgctgcgc aaaaacctca actgcagtca ccaagggaat gcggaaccgt tggtccgcag
    36541 ccttctagcc agtgccggac gtctcgccat cgatcgtaca cccatgtgtg aaagactggc
    36601 agggtggtat cctgcttacc gtcttcgctc agcccaaggc ttcccggacg gcctgggttg
    36661 gtctacacgg cgatgcagtc aaaattcgca tcgcggcatc tcccgtcgac ggagcagcaa
    36721 accgcgagtt gatccgtttc ctcgctgagg agttgtcgat gcctatgagc gctatcgaca
    36781 ttcactccgg tgccggtgga aggcacaaac gcgtctgggt aaggggaacg acagcggtgg
    36841 acgtcacggc gtatctgata cggaaatgtc agggataagg atgaacctgt tcagagcctt
    36901 ggtgcttatg gtcgggacgt tgttgtgtgc ctgttcagga ccgaaaccgg tcctgtatcc
    36961 gaacgcacac ttgcaatcgg tggggaacga gacggcggag caggacatcg aagcatgtaa
    37021 agaagcggct gaaaccgccg gtgcggatga aggcagtggc aaggccggac gtgtcgcgac
    37081 aagtacgggg gttggcgccg gcgttggtgc agccagtgga gcggtcggag gtgcgatctc
    37141 cggagcggcc ggtcaaggat ccctgattgg agcagccagt ggagcggtgt ggggtttatt
    37201 gacgggtctc ttcactgccg gcagctctca accgagccag gccttcacga attatgtgaa
    37261 tcggtgtttg caggaacggg gatatgaggt gaccggttgg caataacggc gagcaggttg
    37321 ggcaatgcac atgaaacagt cattcgtgac gtgtctaaaa aatcctccgc ctatccggga
    37381 gatgacagga tgccgaaccg gtgaacaaga tcctcatatc atggcttgtt attgcgctcg
    37441 ccggctgttc gggacccgag ccgatgctgc gggcaaacgc caagttgctg ttgaaaggcc
    37501 gcgaagcttc ccatctggaa gtcgccgcgt gccagcagaa ggcagaagca gccggcctac
    37561 agcccggcac tgccgggaga gcgggaaaca tggctgcggg cgcgggactg ggactgatcg
    37621 ccggtgcagc ggtcggggcg tcgaccgggg tgatcggggg tgcaacgggc gtggccatag
    37681 gcgcagctgc gggcgccggc cttgggctca tcatcggatt ggccggcggg gcctaccgac
    37741 cgctagaacc tgatccacct tatgctgatg ccgtggtgcg atgtttgatc gagcagggat
    37801 atgaagtcac ggggtggcac taattcaatc cgttgtgtcc ggcgaggcgt ccgaggcctt
    37861 atggcgtagg gcccgaccct tgtccggcgt gggatcggta atcacaccat acacttcccg
    37921 tccttcgtga ccttccggaa gatattccgt gatcggcatt ccgtcttccc ggacaaacaa
    37981 taagcagtgg caatatttgt aaatctgcat ttcgtcgcag gcgcagatcc atcggcgttg
    38041 tttggcctcg gcggctttat ctttgtaaaa attgcagggg cacagcggct ttcccagctc
    38101 gtcaacgtgc atggccaggc ctttgaccac ggactccgtg acggcccgat tggggtgcat
    38161 cgtggtgccg ctcttcgcgg cgaagctatt tacgtacttc cacattttgt ccaggctggc
    38221 ttgtttgggt tctgccatcg tcagttgtcc attggcggtg atgtcggctc gaggcgatat
    38281 cagcgtctgg cgccagttta caagcgttgg gcggtccttt acaatccgca acttcgcccc
    38341 atgttgcgca aattgctggg aattccccga ggctcgcttg cgcgttgact cctaaacgcg
    38401 acaggtcgaa caccctcctg tatggacgac gggactaacc gagacggctg agaaggatga
    38461 cgagcggtgg agtctgcgaa ccttcaattg atcaaccgat tggccggtga gcttgacgct
    38521 gtcgaggccg ctgcactcgt gccacggatc gtggcagctg cgcacaaacc agcagcgatc
    38581 ctggccttgc tggatgaact tgaggccata tccgccaagg cggtccgagc cgccatcgaa
    38641 gcgctgcctg aattggaccg gcgtgccggc tgttcacaga tccacgcgtg gctcgatctt
    38701 ggggtagccc tcgctgggtc atccggtgcc atcgcgctga agtatttcaa ggacagcccg
    38761 cttatctttg gtctcatcga gcgatccgac cgacaggccg cggtgttggc gatggggctt
    38821 gaactggccg agcacgacgc caatgtcagt tgggagtatc tgaaaacagc accacagatc
    38881 gcgaccgcgc ttcctatgga tcagtggccg gcttggctgg acatcggcct ggagatgacc
    38941 cgcctggagc cggtcgtcgg cctcgaatat atcaggcaga ttcccgctct ggtgccggtg
    39001 cttggcgcac cagacgtgcg gcagtggttg ggcttcggga tgaagctgat catgccgaat
    39061 tcattgggaa agccggatta cctcgccacg atggaattct tgcgaacgag cccggctatt
    39121 ttgagcgaca tcgaagcgcc gctgcgctct tctgtcattt cagtgggcgc gctgttggcc
    39181 gaacgggccc cggagactgg catcgcctgg ctcgctgaat cgccaagact cctgcgcctc
    39241 ctaccttcgc ctccctggag aaccaaggta gtgcagtatg gagcgttgtt ggcggagaag
    39301 gatgccgacg cagccttgtc ctacctacgg cgtgggcctg aacttgtcgt gttgatcggc
    39361 gagggacccc agacggatgc tcgctttgaa gagtggttca gggccggcat ggaggtcctg
    39421 gaatatagtc cggaaggcgc gcgggcatat tttgccaccg agtccctcaa ggcgctttca
    39481 tcggtggagc acgcgctgag cggcgttccg cttcgacaag tcgctcgtca gatcaagctg
    39541 gtcgtgcaag gcctctgcgg atccgatgtc tccatcatcg cgcggccgga agcgttgact
    39601 acggcaataa tccgccccat cgtgagcgcc gacgggaaga cgattgcgct gccggctctc
    39661 ctgcgccgct atcccactgc cagacaaaac gaacggctct atctcgtcat ggcagcccac
    39721 gaggccgggc atttagagtt cggcacgtat gcattgtcaa tgacttcgct tctggatctg
    39781 ctcgaggcgg tctgccggcg ttacgggagg ccgcgcccgg ccaccctgga gtcactcgct
    39841 gcactcttcc gtctctatcc tcatccgcgt ctcatgcagg atctctggac gatcttagaa
    39901 gatgctcgcg tcgagtcttt ccttcagcgg gagtatccgg gctttcgaag agacctggca
    39961 caattcgctg cagacacgat cctccctcgt gatcctgccc acggcttgat ggtgcgggag
    40021 ctgatcgccg attgtctgtt gcgcctctcc gttggagagt cgatgtcctc ggcggttcct
    40081 cgcgccgtca tggaagaggt ctccattctt tgggacttgt gccaatccgt attgacggcc
    40141 gactcgacag cagagcgcag cgtgcgcacc gctcacgccg tgtacgtgag aatggaagaa
    40201 ctattggcct cccgtgcgga cctgcttgag tcggaacagc cggccgacga ttcgacggaa
    40261 actgccagcg gccaaagcgc cccagaacag gcagacaatg actatcgtcc cgtgagcaat
    40321 gcgttttatc ggggggagat gcacccggaa atgatcggag gagatgtcga gaagtcggac
    40381 tcggaggtcc agccgcccgg aggcggctca acagaacgga tacccgcgga gagaggccga
    40441 ttcggagaag aacattcgct cggtggtgac ctgcttggcg gcgggcactc cttgccggcc
    40501 gtcgttgagg aactcctga
//
LOCUS       DASSCQ010000076        13416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1494575, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000076 DASSCQ010000000
VERSION     DASSCQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13416
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494575"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFH05_05225"
     CDS             complement(<1..319)
                     /locus_tag="VFH05_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677646.1"
                     /translation="MLFSMIIRLAMVAATTGAVLWIGWTLPSSQFLNSDEIPPLDQPD
                     TNGLGSTDRLRQSQTGFEAVRNRPQSRSSHPSVPRTIDLNLASERDLEGLPGIGPILA
                     LRII"
     gene            complement(532..921)
                     /gene="gcvH"
                     /locus_tag="VFH05_05230"
     CDS             complement(532..921)
                     /gene="gcvH"
                     /locus_tag="VFH05_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET6677647.1"
                     /translation="MIPTDLRYHQEHEWVRVKGAQATVGISHFAQDALGDIVFIDLPK
                     AGATVKAGQQIGEIESTKTTSTLYTPVSGTIAQVNAQLKDHPEVVNSDPYGQGWMAVI
                     DLSNAAELDSLMTAAQYEAFLANQKQG"
     gene            complement(943..1362)
                     /gene="ndk"
                     /locus_tag="VFH05_05235"
     CDS             complement(943..1362)
                     /gene="ndk"
                     /locus_tag="VFH05_05235"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET6677648.1"
                     /translation="MSERTLAIIKPDAVNKHVIGDIVSRYEKAGLKPVAMRLMQMGKP
                     VAEGFYAVHKARPFFDSLCTFMSSGPSVVLVLQGENAIKKNRELMGATDPAKAEKGTI
                     RAAHGTNIEYNAVHGSDSPETARFEITYFFPGMDIIG"
     gene            complement(1521..2549)
                     /gene="mtnA"
                     /locus_tag="VFH05_05240"
     CDS             complement(1521..2549)
                     /gene="mtnA"
                     /locus_tag="VFH05_05240"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET6677649.1"
                     /translation="MVPTVEWHKGAVRLLDQSRLPETVAFLDCRDVQAVASAIRELKV
                     RGAPAIGVTAAMGVALGAQGIRATDYDAFVKALLPICDELAATRPTAVNLFWAIDRMK
                     RKLSALGSESVPSIKAALMGESQAILDEDIALCKSMGRHGADLIQGGQTILTHCNAGA
                     LATAGYGTALGVIRAAWEQGKKIEVIADETRPVLQGARLTAWELMQDNIPVTLITDNM
                     AGSLMREGKIHLCVVGADRIAANGDVANKIGTYSVAVLAKAHGIPFYVAAPYSTIDLN
                     TKSGADIPIEQRNPSEVTTIHGSQPVAPAGVNVYNPAFDVTPAELISGIITERGVFKP
                     QDLAQRFG"
     gene            complement(2628..2828)
                     /locus_tag="VFH05_05245"
     CDS             complement(2628..2828)
                     /locus_tag="VFH05_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677650.1"
                     /translation="MAKLVRIVIQLPLDLKTRLDGLKQQGYTTSGFIRAMLERELAKI
                     DDQSPEQKSLTLVPTIKRANSR"
     gene            complement(3002..5539)
                     /locus_tag="VFH05_05250"
     CDS             complement(3002..5539)
                     /locus_tag="VFH05_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET6677651.1"
                     /translation="MLPSLTLAFICGLALGSLVPYFPLSIVLGLIGAVLGSAMLQAHG
                     RSTAGISTAHYGCLLAGIVYWFASVEAPSNPAILEHVQETFETRTGRIISPVQLAPDR
                     MSMVIRCDPTPGGEGLPLTVRLTWRAPDRPLFEGDRIRLRARLRAPSGASNPGGFDYA
                     SYLERQGIDAVATVSGVEAIEVLESGRADPHWAVWNQFDRWRASIRRSALESLSQPAL
                     GLYLGVIIGERGYLESEVRDQFMVTGTVHLLSISGSHLGLVAMITFIAVRRGLLLLPA
                     YWLLALSRWITPTRAAAVATVAPVTAYACLAGAELATVRSLLMVLVALMAKWMGYERR
                     MFHALSAAALIIVAHDPQAIYDISFQLSFLSVSAVAWWLAKTEGAGDPNESPRSKPAL
                     IGRWIGDAVLMSAVVTVVTLPLVALYFNQFPWVGLLTNLAAVPVMGGLLVPMGLAAVV
                     WQGVAGGDSLPCAEAIQWSMDALVLALRLISVLPGTEWHGASPSLPSLVLFYGCLAAL
                     AIWSDRRSIRWSTATGLLLLLLWWLWSPRLMLDGDRFRITFLDVSQGDSAVLELPGGE
                     VVLIDGGASYERFDMGRSVVAPYLWNRGIRTIDYVIATHPQLDHVGGLTYVLRHFNVR
                     HVWGSGDARRELFYQRLTETLVQQGLTEHVVRAGRDTVMLGDCALKVLNPFAPPVQRQ
                     ASSRRLEGHRLNNRSVVTQVTCGTHKMLFAADVEQEALSRMSQDEQSERVDVVKVPHH
                     GAGSSLQPEWLDRINPAYAVISVGRHNSYGHPADAVLQAYRARGISVYRTDRDGGVWL
                     TGRRSQPALKIHRMNDERIRPLSLSACSWACEQSNWERLLHRWMEES"
     gene            complement(5586..6935)
                     /gene="glmM"
                     /locus_tag="VFH05_05255"
     CDS             complement(5586..6935)
                     /gene="glmM"
                     /locus_tag="VFH05_05255"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET6677652.1"
                     /translation="MRKLFGTDGVRGTANLEPMTSETAMQLGRAAAHIFMRRAGRHQI
                     VIGKDTRVSGYMLEAALMSGICSMGVDVLLVGPMPTPAIAFLARSLRADAGVMISASH
                     NPYQDNGIKFFSNDGLKLPDEIEARIEELIISNEIAHLRPTADAIGKAFRIDDAEGRY
                     IEFVKRSLPKELDFQGIKLVVDCANGAAYKVAPTVLRELGAKVDVIGNKPDGMNINAG
                     CGAVHPTLLQRAVKEYGAHLGIALDGDADRAIFVCEQGNVIDGDHIMAALGLDLHRKG
                     QLAKQTVVGTVMSNFGLELALNRAGLKLLRTAVGDRYLLERMLGDGYNFGGEQSGHFI
                     FLDHNTTGDGLISALQVLSLMKRTEQPLSELAKAMTAVPQVLLNVKVSKKPHLDSIPE
                     LQQAIAESERRFNGSGRVLVRYSGTEPVLRIMIEGEHDSLIREEADRLARIVTAHLS"
     gene            complement(6928..7788)
                     /gene="folP"
                     /locus_tag="VFH05_05260"
     CDS             complement(6928..7788)
                     /gene="folP"
                     /locus_tag="VFH05_05260"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6677653.1"
                     /translation="MELHLPERRLSLDRPLVMGIVNVTPDSFFDGGRFLDPQLALEHG
                     RRLVEEGADLLDIGAESTRPGSVPIDDEEELRRLVPIVTALAQAVSVPISVDTSKAAV
                     AKAAIKAGAVMVNDVTALRGDPDMADVVAQTGVALVLMHMQGTPQTMQQAPTYGDVVE
                     EVADFLAERARFAMERGVAKSRIVLDPGIGFGKVLTHNLDLLAHLRMLTTLGFPVLVG
                     PSRKGFIGQLTKQSVEGRAWGTAGVVALAVEQGANILRVHDVAAMRDVVNVATAISGR
                     MPASLREQHA"
     gene            complement(7991..9802)
                     /gene="ftsH"
                     /locus_tag="VFH05_05265"
     CDS             complement(7991..9802)
                     /gene="ftsH"
                     /locus_tag="VFH05_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET6677654.1"
                     /translation="MNSRAKNLLFWVVVGLFMILLFNLFSVPTHAPEEEVIFSDFMAK
                     LDKGEIEKVIIKSSHVSAVLKDKTRIRTFTAEYPDFVKVLREKGVQIEAKPPDESPWY
                     ITFLVTWGPFVLFLGLWFFLMRQMQIGGNKALSFGKSRARMLTEERKKVTFSDVAGID
                     EAKEEVLEIIEFLKDPRKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTTEGVILIAATNRPDVLDPALLRPGRFDRQVVVNRPDLRGRSEILK
                     VHTKKVPIATNVELEKIARGTPGFSGADLENLVNEAALWAARQNKKEVEVIDFEMAKD
                     KVLMGAERKSMILSDEEKRTTAYHEAGHALMAKLLPGTDPVHKVTIIPRGRALGVTMQ
                     LPTDDRHNYSKEFLYNNLAILMGGRVAEELVLKHVTTGAGNDIERATDLARKMVCEWG
                     MSEKLGPLTFGRKEEEIFLGRELTTKRDFSDQVALEIDLEIKRLVTENYERAKRLLTE
                     HMRSLKALAEALLEKEVLDAPEIDQILMQNSSQTVPA"
     gene            complement(9905..10444)
                     /gene="hpt"
                     /locus_tag="VFH05_05270"
     CDS             complement(9905..10444)
                     /gene="hpt"
                     /locus_tag="VFH05_05270"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET6677655.1"
                     /translation="MERIFGRPIVTQEEMRARIKELGKQIANDYAGKDLVLVGVLKGA
                     YAFYADLARAIRIPMRVDFLIVASYGSQSKTSGKVKVITELTEQIKGKDVLLVEDIVD
                     SGLTVEYLIKTLSKQKPKSIKVCTLLSKPERRTIDVPLEYVGFKIPNKYVVGYGLDYQ
                     QKYRNLPYLAELDMEGEQE"
     gene            complement(10446..11909)
                     /gene="tilS"
                     /locus_tag="VFH05_05275"
     CDS             complement(10446..11909)
                     /gene="tilS"
                     /locus_tag="VFH05_05275"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET6677656.1"
                     /translation="MAVATTTRSRHPALHGMVRTIRDHQLFEPGQHLLVAVSGGPDSV
                     ALLSLLTALAPAWHLTLTVVHFNYGLRGTESDADEAFVAALCRERGISLVVQRPVLTK
                     RRGAASLQARARTARYRAMADLADDIRADRIVTGHTANDQAETVLLWLLRGAGLTGLS
                     GMRFIRNQRIIRPLLEITRHDVLDYLKREGVRYREDSSNATSLYRRNRIRRELLPVME
                     QIAPAIVRRLERQSHVLRADDEYLEEIVDHVYRSTVIVEPSGNQRFERQMFARLPAAL
                     QRRLVRRSLRAGDPEGRAPSLRTVENVLRLVAGKSKGMLSVRGLDVITEREWIVLRLT
                     PQRTVSSPSGQSFPTVTVPISLPATVYWPGTRQQIHVQVMTKQAAAPWLKQGTPDCAV
                     FDADRLSAPLIIRAWQAGDRIHPRGMRGKRKKLQDIFTDMKVSRDERSRIPLLVAPEG
                     ILWVVGRRQDERCTVGKDTRRCLVVTVQSKAGSEGAG"
     gene            complement(11910..12845)
                     /locus_tag="VFH05_05280"
     CDS             complement(11910..12845)
                     /locus_tag="VFH05_05280"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET6677657.1"
                     /translation="MTRDLGDARSRPHPVVTIGNFDGHHRGHQSLLQTVVERARAVTG
                     TAMVLTFDPHPVRVLAPHVELRFLTSPEEKLARFKAAGIDEVVFLEFTPALAAMTPEE
                     FADIVLHRRLRVSDVYVGEHFAFGRGRAGRIADLERFGRMLGFTVHPVKPVMLNGGVV
                     SSTRIRTFIQAGRMDEAAVLLGRAYGITGTVVRGTQKGHALGWPTANLRIPPERVVPP
                     DGVYAARAAWEGRASDAIAYIGTRPTFGSGERLIEVNVLDQRANLYEKEMSVEFVERL
                     RGDHAFASAEELAAQIAVDVDRARVSLRRFAEGVR"
     gene            complement(12860..>13416)
                     /gene="hemB"
                     /locus_tag="VFH05_05285"
     CDS             complement(12860..>13416)
                     /gene="hemB"
                     /locus_tag="VFH05_05285"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET6677658.1"
                     /translation="NDETLECLRAMARTHAQAGADMVAPSDMMDGRVAAIRSELDHAG
                     YPELPIMAYAAKFASCFYAPFRDAAGSAPQFGDRQSYQMDPANRREALREIALDVEEG
                     ADIVMVKPALPYLDVIAAARAHTALPVAAYQVSGEYSMIKAAGKAGWLDESRAMLESL
                     LAIKRAGADLILTYFAKDAAPFLR"
BASE COUNT         2764 a         4101 c         3792 g         2759 t
ORIGIN      
        1 cgataatccg tagggccaga atcggcccga ttcccggcag cccttcaagg tcgcgctcag
       61 acgcgagatt caaatcgatt gttcgaggca ctgatgggtg tgacgatcgg ctttgcggcc
      121 ggtttcggac tgcctcgaat cctgtttggg attgccgcag gcgatctgtg gagcccagcc
      181 cattcgtgtc gggctggtcc aacggcggga tctcatctga attgagaaac tgcgaggagg
      241 gaagggtcca tccgatccac aacacggcgc cggtggtggc ggcaaccata gccagcctga
      301 tgatcattga gaacagcatc aaccatcctt ctggagcaac tgacgggctc tgctcaccct
      361 atgcgcacgc agaacttttc tcgtcgacga ctccccaagt tgcgccgctc tcatcgctgc
      421 gacctgaggg ctgcactcag aatgatgatg tgggatcgac tgcgcatagc aagattgcgg
      481 gcggaggtga aaggttacga tgaacctgct cccggtttcg gaagcggcaa cctaaccctg
      541 cttctgattg gccagaaaag cttcgtattg tgccgcggtc atcaatgagt ccagttcagc
      601 ggcattggag agatcgatca ccgccatcca accctggccg tacggatcac tgttgaccac
      661 ctcaggatgg tccttcagtt gggcattgac ctgcgcaatg gtaccgctga ccggcgtata
      721 caaggtggac gtggttttgg tcgattcgat ttcgccgatt tgttgcccgg ccttcaccgt
      781 cgccccagcc ttcggcaaat caatgaagac gatatcaccc agggcatcct gcgcaaaatg
      841 actgataccc acagtggctt gagccccctt gactcgtacc cattcatgct cttgatgata
      901 gcggaggtcc gttggaatca tgatggtgat gtcctcattc cgtcagccga tgatgtccat
      961 tccgggaaaa aaatatgtga tttcaaacct ggccgtttcc ggcgagtccg atccatgcac
     1021 ggcgttgtac tcgatattcg tcccgtgtgc agcacgaatt gtgcccttct cagccttggc
     1081 agggtccgtc gcacccatca gttcacgatt tttcttgatg gcgttttcgc cttgcaggac
     1141 gagcacgact gacggccccg acgacatgaa ggtgcacaga ctgtcgaaaa agggccgtgc
     1201 cttgtgtacc gcatagaatc cttccgcaac cggtttgccc atctgcatca gtcgcatcgc
     1261 caccggcttc aaaccggcct tttcataccg gctaacgatg tcaccgatca catgtttgtt
     1321 cacggcgtct ggcttaatga tcgcgagcgt gcgttcactc attgcttgaa ctcttcctct
     1381 catctcgaga caacaaatgc gaaaaatatc ggcgcattat aggggcttgg aatgcggagt
     1441 ttcaagggag gaacagcgac cgcgcttttc ccgaatcgta cacccctgcc ttaccgagcg
     1501 cggcggctgc cgctgacagg tcatccaaag cgttgcgcga ggtcttgcgg tttgaagacg
     1561 ccacgctcgg taatgatgcc ggaaattagc tcagccggcg tcacatcgaa cgcgggattg
     1621 tagacattga ctccggccgg ggcgacaggt tgactgccgt gaatggtggt gacttccgag
     1681 gggttgcgct gctcgatcgg gatgtcggcg cccgattttg tgttcagatc gatagtcgaa
     1741 tacggcgccg caacatagaa cggaatgcca tgcgccttgg ccaggaccgc caccgagtaa
     1801 gtaccgattt tgtttgccac gtccccgttc gctgcaattc ggtccgcccc caccacacag
     1861 agatgaatct tgccctcgcg catcaaggac cctgccatgt tgtctgtgat cagggtaacg
     1921 ggaatgttgt cctgcatgag ctcccatgcc gtgaggcgcg cgccctgtaa tactggtcgc
     1981 gtttcgtcgg caatgacctc aatttttttc ccctgttccc aggccgcgcg gatgacaccc
     2041 aacgctgtcc cgtaccccgc cgtcgccaag gcgccggcat tgcaatgagt gaggatggtt
     2101 tggcctccct gaatgaggtc ggcaccatgg cgacccattg acttacatag ggcgatatct
     2161 tcgtcgagta tcgcttgcga ctcgcccatc aaggccgctt taatcgacgg gaccgactcg
     2221 gagcccaggg cagagagttt gcgcttcatg cggtcgatag cccagaaaag attgacggcg
     2281 gtcggccgtg tggcggccag ttcatcgcag atcggaagca gcgccttcac gaaggcatcg
     2341 taatcggtgg cgcgaatgcc ttgtgctccc agcgccactc ccatggcggc agtcacccca
     2401 atcgccggcg cgccgcggac tttcaattcc cgaatggctg atgccacggc ttggacatcg
     2461 cggcagtcca ggaatgcaac cgtctccgga aggcgactct ggtcgagaag gcgtacagcc
     2521 cccttatgcc attcaacggt cggaaccata ggcgactcta tgtaacggga ttcgtatggg
     2581 gagcgcaaga ggctgggtcc aggaggaacg ggaggaatga agctggatta tcgactgttg
     2641 gcccgcttaa tcgttggaac gagcgtcaag gacttctgct ccgggctctg atcgtcgatt
     2701 tttgcgagtt cacgctcgag catggcacga atgaacccgc tggtcgtata gccctgttgt
     2761 ttgaggccat ccaaccgggt ttttaaatct agcggaagct ggatcacgat acggacgagc
     2821 tttgccatgt gggacccctt atcagaagct gatccaaaga ggtgccgatt gcacacgctg
     2881 tgccttacta gcacgaatac ggcgctgtgt agaattgagg acttagcgat aagaagctgc
     2941 ctgtcatcgc tccgcttacc taaaattcgt ctgcacatgc gctcaaatgt acaagagccg
     3001 gtcacgattc ctccatccaa cggtggagca gccgttccca gtttgattgt tcacaggccc
     3061 atgagcaggc agaaagagac agcggtcgga ttcgttcgtc gttcatacga tggatcttga
     3121 gcgccggttg tgagcgcctt cctgtcagcc agaccccgcc gtcgcggtcc gtgcgataga
     3181 cggaaatccc acgagcgcga taggcctgca ggacggcgtc ggccgggtgg ccataggaat
     3241 tgtgccgccc gaccgaaatg acggcatagg ctggattgat ccggtcgagc cactcgggct
     3301 gcaacgaact tcctgcgccg tggtgcggga ctttcaccac atccacccgt tcagactgtt
     3361 cgtcctggct catccgcgac aaggcttcct gttcaacatc ggcggcgaac agcatcttgt
     3421 gggtgccgca agtcacctgc gtcacgacag agcgattgtt gagcctatgc ccttcgagac
     3481 ggcgagagga cgcctggcgc tgcacaggcg gagcgaacgg attcaacacc ttcaaggcgc
     3541 aatcgcccag catgaccgta tcccggccgg cacggaccac gtgctcggtc aagccttgct
     3601 gcaccagcgt ttcggtgagc cgctgataaa acagctcccg gcgcgcatca ccgcttcccc
     3661 acacgtgccg cacgttgaaa tgcctgagca catacgtgag gccgcccaca tgatccaatt
     3721 gcgggtgcgt cgcaatgaca taatcgatcg tcctgattcc acgattccac aggtagggtg
     3781 ccaccacact gcgtcccata tcgaaccgct cataactggc tccgccatcg atcagcacga
     3841 cctccccgcc tggtagttca agcaccgcac tgtcgccctg cgacacatcc aaaaatgtga
     3901 tgcgaaagcg atcgccatcg agcatgagtc gtggtgacca cagccaccag agaaggagca
     3961 gaaggagacc ggttgcggtg ctccaccgga tgcttcttcg atcggaccag atggcgagcg
     4021 cggccagaca tccgtaaaag agcacgagag acggcaggga tggcgaggcc ccgtgccact
     4081 cggtgccggg cagtaccgag atcaagcgca gggctaggac cagggcgtcc atggaccact
     4141 ggatcgcttc tgcacacggc agactgtctc ctccggctac gccttgccag accacggcag
     4201 caagtcccat tgggaccaac aggccgccca tcaccggcac cgcagcgaga ttcgtgagca
     4261 ggccgaccca tgggaattga ttaaagtaca gcgccaccag cgggagcgtc accacggtca
     4321 cgaccgcgct catgaggacc gcatccccta tccaccggcc tatcagcgcc ggcttcgacc
     4381 gaggcgactc attggggtca ccagcgccct cagttttcgc gagccaccag gcaacagcac
     4441 taactgaaag aaacgacaac tggaatgaga tgtcgtaaat ggcttgagga tcatgggcca
     4501 cgatgatcaa cgccgccgcg gacaaggcat ggaacatccg tcgttcatag cccatccatt
     4561 tcgccatcaa ggcgacgagt accatgagaa gcgagcgaac agtcgccagc tccgctccag
     4621 ctaaacaggc atacgccgtc actggcgcga cagtcgcgac agcagcagcc ctcgttggcg
     4681 tgatccaacg ggacaaggcc aggagccagt aggccggaag cagcagcagg ccacgcctga
     4741 ccgctatgaa cgtgatcatg gcgaccaagc caagatggct ccccgatata gacaagagat
     4801 gtacggtccc cgtgaccatg aattggtccc ggacttcaga ctcgagatat cctcgctcgc
     4861 cgatgatgac cccaagatag agaccgagag ccggctgaga cagactctcc aacgccgacc
     4921 gacgaatcga ggcccgccat cgatcgaatt gattccacac agcccagtgc gggtcagcac
     4981 gtccagactc cagaacctcg atggcttcca ccccgctcac tgtggcgacg gcatcgatgc
     5041 cttgacgctc gagataggag gcataatcga atcccccggg gttggacgcg ccgctcggag
     5101 cccgtaatcg tgctcgaagc cggatgcgat caccctcgaa gagcggccgg tccggggcac
     5161 gccacgtcaa gcgaaccgtg agcggcagac cttcgccgcc aggagtcgga tcacacctga
     5221 tcaccatgct cattcgatcg ggagccagtt ggacgggcga gataatccgt cccgtgcggg
     5281 tttcgaacgt ctcttgcaca tgctcgagga tggccgggtt tgagggggct tcgacgctcg
     5341 caaaccagta gacaatgccc gccaagaggc atccatagtg cgcagtcgat atgccggctg
     5401 tggatcgtcc atgcgcctgg agcatggccg agccgagaac agcgcctatc agtccgagaa
     5461 cgatcgacaa cggaaaataa ggaacgagag agcccagcgc aaggccgcag atgaaggcaa
     5521 gcgtgaggga cggcagcatg gcttccgttc caacagtgat gacgtgatgt ggctgaacca
     5581 ggaaactagc tgaggtgggc cgtgacgatt cgtgccaacc gatcggcctc ttcgcggatg
     5641 agactatcgt gttccccttc gatcataata cgcagcacgg gttcggtgcc ggaatagcgg
     5701 acgaggaccc ggccgctgcc gttgaaacgg cgttcgcttt cggcgatcgc ttgctgcagt
     5761 tcggggatgc tgtctaaatg tggcttcttc gacactttga cgttcagcaa gacttgaggg
     5821 actgccgtca tcgctttcgc cagctccgac aggggctgtt cggtccgttt catcagcgag
     5881 agcacttgca gcgcggaaat cagcccgtcg cccgtcgtat tgtggtcgag aaaaatgaaa
     5941 tgcccggatt gctcaccgcc gaagttatac ccgtctccca acatccgctc caataagtat
     6001 cggtcaccga cggctgtgcg gagtaacttg aggcccgccc gattgagcgc cagctctaag
     6061 ccaaaattgc tcatgaccgt tccgaccacc gtctgtttcg ccaactggcc tttgcggtga
     6121 agatcgagtc ccaaggcagc catgatgtga tcgccgtcga taacattgcc ctgctcgcat
     6181 acgaagatgg cgcggtcggc gtcgccgtcc agcgcaattc ctaagtgtgc gccgtattcc
     6241 ttcacggccc gctgcagcag cgtcgggtgc acggcgccgc atccggcatt gatgttcatg
     6301 ccgtccggct tattcccgat cacgtccacc ttcgcgccta actcacgcag aacggtcggt
     6361 gccaccttgt aggcagcgcc attggcacaa tcgaccacaa gcttgatgcc ctggaaatcg
     6421 agctctttgg ggagtgaccg tttgacgaat tcgatatatc tcccctccgc gtcatcgatg
     6481 cggaacgcct ttccgatggc atcggccgtc ggacggagat gggcaatttc attcgaaatg
     6541 atgagctctt caatcctggc ttcgatttca tccggcagct tgagcccgtc gttcgaaaag
     6601 aacttgatgc cgttgtcttg atacggattg tgtgaggcgg aaatcatgac gccggcgtcg
     6661 gcccgcaggc ttctggccaa aaaggcgatc gccggggtgg gcatcggccc gaccagcagt
     6721 acgtcgacac ccatcgagca gatgcccgac atcaaggcgg cttccagcat gtagcccgag
     6781 acgcgtgtat ccttcccgat cacgatttga tggcgtccgg ctctccgcat gaaaatgtgg
     6841 gcggcagcgc gcccgagctg catggcggtt tcactggtca tcggctcaag gttcgccgtc
     6901 cctcgcacgc cgtctgttcc gaacaattta cgcatgctgt tcccgtaatg aagccggcat
     6961 tcgacctgaa atcgccgttg ccacgttcac gacgtcccgc atcgccgcta cgtcgtgcac
     7021 acgcagaatg ttcgcccctt gttctactgc gagggcgacg acgcccgcgg ttccccaggc
     7081 ccgtccctca accgattgtt tggtcaactg gccgatgaac ccttttcttg atggaccgac
     7141 caacaccgga aaccccaagg tggtcaacat gcgcagatgc gcgaggaggt caaggttgtg
     7201 cgtgaggact ttaccaaatc cgatgcccgg atcaaggaca atccgtgatt tcgcgactcc
     7261 acgctccatg gcgaagcgcg ctcgctcggc caaaaaatca gcgacttcct caaccacgtc
     7321 cccgtaggtg ggcgcctgct gcatcgtttg gggagtgccc tgcatgtgca tcagcaccag
     7381 ggcgactccc gtctgagcca cgacgtccgc catgtcgggg tcgccacgta atgcagtcac
     7441 atcattgacc atgacggcgc ccgctttgat tgcggccttg gcgacagcgg ctttggaggt
     7501 atcgacggag atgggaacgg acacggcctg tgcgagcgcc gtgacgatcg gcacgagtcg
     7561 acgcaactct tcctcgtcgt cgattggaac tgagccaggc ctggtggatt cggcaccgat
     7621 gtcaagaaga tcagcgcctt cttcgaccaa ccgccggcca tgctccagcg ccaattgagg
     7681 atcaagaaaa cgcccgccgt cgaagaacga atccggcgtc acattgacaa tgcccatcac
     7741 caagggacgg tcgagggaaa ggcgtcgctc tggaaggtgg agttccatcg ttgcagttat
     7801 gagttcgatg cggcgagatt ccacgtcctg ttgggatcac gatacagtcc tgtatcttca
     7861 atgacacggg tcatacgtac ctacctcatt gaaatatctg ggcagcgcag cagacgaggc
     7921 gtcatggaat gcatgaggta tgatctcctg caactccagt gccctgaagc tgcagtgaca
     7981 ccggcagcga ttaggcaggc acggtctgcg aggagttctg catcaggatc tgatcgatct
     8041 ccggggcatc caacacttcc ttttccagaa gggcttccgc gagcgccttc aggcttctca
     8101 tgtgttcagt aaggagccgt ttggcccttt cgtaattttc ggtgaccaac cgcttgatct
     8161 cgagatcgat ttccaatgcg acttgatcgc tgaaatcccg tttggtggtc aactcccggc
     8221 cgaggaagat ttcctcctct tttctgccga aggtcaacgg ccccagcttt tcgctcatgc
     8281 cccattcaca gaccattttt ctggccagat ccgttgcgcg ctcgatgtcg tttcccgcgc
     8341 cggtggtgac gtgtttcaac acgagctctt ccgctacgcg cccgcccatg agaatggcga
     8401 ggttgttgta caggaattcc tttgaatagt tgtgccggtc gtcggtcggc aactgcatcg
     8461 tgacgcccaa ggcccgcccg cgcgggataa tcgtgacttt gtggacggga tcggtgccgg
     8521 gcaggagttt ggccatcaag gcatgccccg cctcgtgata cgcggtcgtc cgtttttctt
     8581 cgtcactgag aatcatgctc ttgcgttcgg cgcccatcag gaccttgtct ttggccatct
     8641 cgaaatcgat gacttcgact tccttcttat tctgacgcgc cgcccacaat gccgcttcgt
     8701 tcaccaggtt ttccaggtcc gcccccgaga aacccggtgt cccgcgtgcg atcttttcca
     8761 actcgacgtt ggtcgcaatc ggcactttct tcgtatgcac cttcaggatt tcggagcggc
     8821 cgcgcaaatc cggtctattg acgaccactt gccgatcgaa gcgtcccggc cgcaacaagg
     8881 cagggtcgag cacgtcgggc cgattggtcg ccgcgatcaa gatcacccct tccgtggtgt
     8941 cgaacccgtc catctcgacg agcaactgat tcagggtttg ttcgcgttca tcgtgtccgc
     9001 cgcccaagcc ggcaccgcgc aagcggccga cggcatcgat ctcgtcgatg aaaatgatgc
     9061 agggcgcatg ctttttgccc tgttcaaaga gatcgcgcac tcgcgacgca cccaccccga
     9121 cgaacatctc gacgaagtcc gatccactga tgctgaagaa gggcaccccg gcttcgccgg
     9181 caatggcttt agcgagcagg gtctttccgg ttcccggagg accgacgatc aagacgcctt
     9241 tcggaatgcg cccgcccagc ttttgaaact tgcggggatc ttttaagaat tcgatgatct
     9301 cgaggacttc ttctttcgct tcgtcaatgc cggccacatc cgagaacgtc actttctttc
     9361 gttcttcggt cagcatgcgc gcgcggctct tgccgaagga cagagctttg ttcccgccga
     9421 tttgcatctg ccgcatcaag aagaaccaaa gccccaggaa gagcacgaag ggtccccacg
     9481 tcacaaggaa cgtgatgtac catggacttt cgtctggggg cttcgcttcg atctgcacgc
     9541 ctttttcgcg caacaccttc acaaaatccg ggtattcggc ggtgaaggtt cggatgcggg
     9601 tcttgtcctt caggacggcg ctgacgtgac tggacttgat gatgactttt tcgatttcac
     9661 ccttatcgag cttggccatg aaatcgctga aaataacttc ttcttccggg gcatgagtcg
     9721 gtacactgaa caggttgaac agtaaaatca tgaacaggcc gacaacgacc cagaagagca
     9781 gattttttgc ccgagaattc atccacttct ccttatgcct ggcttcctca agtcgccagg
     9841 aaatacgcga gcttgagtga tgttaccaca gccctcttcg aactgacaac cgtgcagccg
     9901 ccgtttactc ttgctcccct tccatgtcca gctcggcgag gtaaggaagg tttcggtatt
     9961 tctgttgata atcaagcccg tagcccacca catatttgtt gggaatcttg aatcctacat
    10021 actcaagcgg gacgtcgatc gttcgccgtt ccggcttgct caacagggtg cagactttaa
    10081 tagatttagg cttttgcttg ctcagagtct tgatcaagta ctcgacagtc agtccggaat
    10141 cgacaatgtc ttcgacgagc aacacatcct ttcccttgat ctgttccgtc agctcggtga
    10201 tgactttgac ttttccagac gttttcgact gactgccata acttgcgacg atgagaaaat
    10261 cgacgcgcat gggaatccga atggcccgag cgagatccgc atagaaggcg taggcgcctt
    10321 taaggacgcc gacgagcaca agatctttgc cggcgtagtc attggcaatc tgtttaccga
    10381 gctccttgat gcgcgcacgc atttcctcct gcgtcacgat cggccgtccg aagatgcgct
    10441 ccatcttatc cggctccttc gctgcctgct ttcgattgaa ccgtcaccac aagacagcgg
    10501 cgcgtgtctt ttcccacggt gcaccgctca tcctgtcgac ggccgaccac ccagaggatg
    10561 ccttccgggg cgacgaggag cggaatacga ctgcgctcgt cccggctcac cttcatgtcg
    10621 gtgaaaatat cttgaagttt cttgcgtttc ccgcgcatgc cacgcggatg gatccgatcg
    10681 cccgcctgcc acgccctgat gatcagcggt gcagacaagc ggtcggcatc gaacaccgcg
    10741 cagtccggcg ttccctgttt caaccagggc gcggccgcct gtttggtcat cacttgtaca
    10801 tgaatttgtt gtcgcgtacc aggccaatac accgtagcag ggagggagat cggcacagtc
    10861 actgtcggga aggattgccc ggacgggctg gagaccgttc gttgcggagt caaccggagg
    10921 acgatccact cacgctctgt gatcacgtca agccctcgga ccgaaagcat acccttggac
    10981 ttgccggcca ccagccgcag cacattttcg actgtacgca gactcggcgc ccgcccctcg
    11041 ggatcgcccg cgcgaagact ccgccgtacc aggcggcgct gcagtgccgc aggaaggcgg
    11101 gcaaacatct gccgctcaaa ccgctgattc ccactgggct cgacgatcac cgtcgaccgg
    11161 tacacgtgat ccacgatttc ttctaagtac tcgtcgtctg ctcgcagcac gtgcgactgc
    11221 cgctccaggc ggcgaacgat cgctggcgcg atctgttcca tcaccggcaa cagctcgcga
    11281 cgaatacgat tgcgccgata cagagacgtc gcgttgctgc tgtcctctcg atagcgcacg
    11341 ccctctcgtt tcagatagtc cagcacgtca tgtcgggtga tctctaacag cggtcggatg
    11401 atccgttggt tacggatgaa tcgcatcccc gagagtcctg tcagacctgc gccacgcaac
    11461 agccacaaga gcacggtttc ggcctgatcg ttcgccgtat ggccggtgac aatgcgatcg
    11521 gcgcggatgt catccgccag gtccgccatg gcgcggtagc gggctgtgcg ggcacgcgcc
    11581 tgaagggacg ccgccccccg tcgcttggtc aacaccggtc gttgaacgac aagagaaatt
    11641 ccgcgttccc gacacagcgc agcaacaaag gcttcgtcag cgtcggattc cgttccgcgc
    11701 agcccgtaat tgaagtgcac aaccgtgagg gtgagatgcc aggccggcgc cagagccgtc
    11761 agcagcgaca acaaggcgac cgaatcaggc ccaccggaaa ccgcgacgag gagatgctgg
    11821 cccggctcga acaactggtg atcacgaatg gtccggacca ttccatgcaa cgcgggatgt
    11881 cgagaccgtg tcgtcgtcgc gaccgccatc tatcgcaccc cttccgcgaa tcggcgcagg
    11941 ctcaccctgg cccggtcaac gtcaacggca atctgggctg ccagctcctc cgcggacgcg
    12001 aacgcatggt ctccccgcag ccgttcgaca aactctacgg acatctcctt ctcatacaag
    12061 tttgcccgct ggtccaagac attgacctca atcaagcgct cacccgaacc aaacgttggt
    12121 ctcgtcccga tatacgcgat cgcatcagac gccctgcctt cccaggcggc tcgtgcggcg
    12181 tagacgccat ccggcggaac cacccgttcc ggagggatcc gcagattggc tgttggccaa
    12241 ccaagggcgt gtcccttctg cgtcccccgc acgaccgttc ccgtgatgcc gtaagcccgt
    12301 ccgagcaaga ccgccgcctc gtccatgcgc ccggcttgga tgaaggttcg aattctcgtg
    12361 gaactcacga cgcccccgtt cagcatgacg ggcttgacgg gatggacggt gaaccccagc
    12421 atgcggccaa atcgttccaa gtccgcaatt cggccggcgc gcccccgtcc gaaggcaaaa
    12481 tgttcgccga catagacgtc cgatacacgt agccggcgat ggaggacaat gtccgcaaac
    12541 tcttccggcg tcatggcggc aagggcgggg gtaaattcga ggaagacgac ttcgtcaatg
    12601 ccggccgcct tgaagcgtgc gagcttctct tccggactcg tgagaaatcg cagctcgacg
    12661 tgaggggcga gcaccctgac cggatgtgga tcgaaggtca gtaccatggc cgtgcctgtg
    12721 accgcgcgcg cccgctcgac aaccgtttgc aacagtgatt gatggccgcg atgatgacca
    12781 tcaaagtttc cgatcgtgac caccggatga ggtctggacc gggcgtcgcc gaggtcgcgc
    12841 gtcaccgtga acgtcatcgt cagcggagaa acggcgccgc gtctttcgcg aagtaggtca
    12901 gaatcagatc cgctccggcg cgcttgatgg cgagcaacga ctcgagcatg gctcgcgact
    12961 cgtccagcca cccggctttt ccggcggctt tgatcatgct gtattcaccg ctgacttgat
    13021 aggcggcgac aggaagagcc gtgtgcgctc gagcggcggc gatgacatcg agatagggca
    13081 atgcaggctt caccatgacg atatcagctc cttcttccac atcaagtgcg atctctcgca
    13141 acgcttcgcg ccggttggcg gggtccattt gataggactg ccggtcgccg aactgcggcg
    13201 cggagccggc cgcatcgcgg aagggagcgt agaaacacga agcgaacttt gccgcgtacg
    13261 ccatgatcgg tagttccggg tatcccgcat gatccaattc cgatctgatg gcggccacgc
    13321 gcccgtccat catatccgac ggcgccacca tatcggcgcc agcctgggcg tgcgtccgcg
    13381 ccatcgcgcg gagacattcc aacgtttcat cgttca
//
LOCUS       DASSCQ010000077         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_348814, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000077 DASSCQ010000000
VERSION     DASSCQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348814"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFH05_05290"
     CDS             <1..722
                     /locus_tag="VFH05_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HET6677659.1"
                     /translation="ACSTFKAALSTTWKNYGVPQSRGALPAGPMVIFVHMASVWVPFT
                     SESKEAIADYDEIQKEITLALRECGRRLGLFLRRRERAASEYRRRNIFELYIEEVVEA
                     CNRLKGGKLPKDKIKMQLQKIANSRTGGLKTDEALGKTGAGPEGLPHSIIVTETGIEG
                     EVQAAQDNAEAATTRPAPEGDLLGGSKPDDKPAQGKSGAVRQELKKGTKKGVRGKSEQ
                     IGLFADKSTVRVRAATPRRRK"
     gene            728..1831
                     /locus_tag="VFH05_05295"
     CDS             728..1831
                     /locus_tag="VFH05_05295"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET6677660.1"
                     /translation="MTTKQRRPSSVEKKLIGLADIVISAADRSKDPTFAIPIRALSNV
                     SFNERKGLIEMGHKKQARSFFNVGMAKKFMQTVLVADALSELQRADLTTSLREIYYRT
                     KHTIQDSHENTFDTQDESDPVIEDLEVSLAALREELHVRAENSGSIVGPVVFGDDGDR
                     VDCARLGKGGYSVPSIVEPEYLEIRRCTADFLLLVEKGTQWNRLSEDKFWRRYNCVLL
                     TGNGQPPRGVRRLARRLHEEHRLPVYVLVDNDPWGYYIYSVVKQGSINLAFESQRMAI
                     PKAKFIGLSSADPERYELPRNVGIKLNEKDIARAKELMNYQWFQKPAWQTEIKRMLTS
                     GLKYELDALANKDFQYLTKKYLPRKLKEKDWLD"
     gene            1971..2294
                     /locus_tag="VFH05_05300"
     CDS             1971..2294
                     /locus_tag="VFH05_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677661.1"
                     /translation="MIVMLYIWLLTLTLMAFGWPAFAADIVPAPDPSPPAAQQPPANE
                     VPRVNPVPSPTDPGMVKQPDTVPHPDSVVTPPVVDPKMAINPEAASPQTEPAPPPRDS
                     SPKKP"
     gene            2291..2572
                     /locus_tag="VFH05_05305"
     CDS             2291..2572
                     /locus_tag="VFH05_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677662.1"
                     /translation="MIDESESTRLSDDVKEMTIAGCRVRLAYCRASHEGWIVKGTLQC
                     GLEDQQGERSIVTEAYPSREEAEQHAVERVTALLGQHTDRSHSRVRNWS"
     gene            2610..3176
                     /locus_tag="VFH05_05310"
     CDS             2610..3176
                     /locus_tag="VFH05_05310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET6677663.1"
                     /translation="MAIIGQAIPDGTYQAFHEDEIRTITLDSYRGRWLVLVFYPGDFT
                     FICPTELEELGALYGDFKKLDAEVIGVSTDSVYVHKAWHDTSPAIKKLPFPLLSDAGG
                     ALSNKLGVYLYREGVALRGSFIFDPDGKLCAYEVHANNIGRSMEELLRKLQAASFVHQ
                     NGGAEVCPAGWKPGDRTLRPSLDLVGKL"
     gene            3496..>3739
                     /locus_tag="VFH05_05315"
     CDS             3496..>3739
                     /locus_tag="VFH05_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677664.1"
                     /translation="MTRTTIITAAGAFMVLSFMSACNRDQDQTTPVGQQQGQGSAQDY
                     PSTRNMEQPNPAIGGTNSATNKPAPSGNQEPQAGSTT"
BASE COUNT          909 a         1067 c         1064 g          699 t
ORIGIN      
        1 ccgcgtgttc cacattcaaa gccgccctga gcacgacctg gaaaaactat ggcgtcccgc
       61 agtcgcgagg ggcgctgccg gccggtccga tggttatctt cgtccatatg gcttccgtct
      121 gggtgccctt tacaagcgag tctaaagagg cgatcgccga ttatgacgag atccagaagg
      181 agatcactct cgccttacgt gagtgcgggc gtcggttggg attgttcttg cgacggcggg
      241 agcgggcggc cagcgaatac aggcggcgca atatcttcga attgtatatt gaagaggtag
      301 tcgaagcctg caaccggctc aaagggggga agctgccaaa agacaagatc aagatgcagc
      361 tgcagaagat cgcgaattcg cgtaccggcg gcctcaaaac cgatgaggcc ctgggtaaga
      421 ccggagcagg tccggaaggc cttccacatt cgatcatcgt caccgagaca ggcattgagg
      481 gagaggttca agcggcccag gacaacgcag aagcagccac aactcgcccg gctccggagg
      541 gcgatctcct tggtgggagc aaaccggacg acaaaccggc tcaaggtaaa agcggcgccg
      601 tgagacagga actgaagaag gggacgaaga aaggcgtgcg cggcaagtcc gaacagatcg
      661 ggttgtttgc agacaaatcg accgtacgcg tgcgggctgc tacgccgcgg cgaaggaagt
      721 aactgccatg accacgaagc agagacgccc gagttccgtc gaaaagaaac tgatcggcct
      781 agccgacatt gtgatttcgg ctgccgaccg gtcgaaggat ccgacgttcg ccatcccgat
      841 tcgcgcgctg tcgaatgtct ccttcaacga gcgcaaaggg ttgatcgaga tgggtcacaa
      901 gaagcaggcc cgttcgtttt tcaacgtcgg catggcgaaa aaatttatgc agaccgtgct
      961 ggtggctgac gcactttctg aactgcagcg cgcggatctc accacgtccc tgagggaaat
     1021 ttactaccgg acgaagcaca ccatccaaga ctcgcatgaa aacacgttcg acacgcagga
     1081 cgaatcggac ccggtcatcg aagatctcga agtctcgctg gcggcgttgc gcgaagaact
     1141 gcacgtcagg gccgaaaaca gcggcagcat cgtcgggccc gtcgtattcg gcgatgacgg
     1201 agatcgggtc gactgcgcac gcctcggcaa gggagggtac tcggtccctt ccattgtcga
     1261 acccgaatat ctggagatcc gacgttgtac cgctgatttt ttgctgctag tcgaaaaagg
     1321 cacgcagtgg aaccgtctgt cggaagacaa gttctggcgc cggtacaatt gcgtcctgct
     1381 gacaggaaac ggtcaacctc ccagaggtgt cagacgactg gcgagacggc tgcacgaaga
     1441 acatcgactg ccggtctacg tcctggtcga taacgatccc tggggctatt acatctattc
     1501 ggtcgtcaag cagggatcga tcaacctggc gttcgagagc cagcgcatgg ccatccccaa
     1561 ggcaaaattc atcggattgt ccagcgcaga tcctgagcgc tacgagctgc cccgtaatgt
     1621 cgggatcaag ctgaacgaaa aggatattgc ccgggcaaaa gaactcatga actaccagtg
     1681 gtttcagaaa ccggcctggc aaaccgaaat caagcgtatg ctaaccagtg gcctcaaata
     1741 tgaactggac gccttggcca ataaagactt tcaatacctg acgaaaaaat accttccgcg
     1801 aaagttgaaa gaaaaagact ggctggattg atacgaagtc gatccgtcca ccactcacgc
     1861 gctagcgtct cgcaaccctt cttcagctga gctaatgatg tcggtttttc tctgcgtgcc
     1921 agcgagcagg ggattcaccc agtaggcaaa ctccgtcgcg gcgagatagc gtgattgtta
     1981 tgctgtatat ctggctgttg actctgactc tcatggcgtt cgggtggccc gcttttgcgg
     2041 cggacattgt gccggcaccg gacccttcgc cgccggcggc tcaacaaccc ccggccaatg
     2101 aggtgcctcg agtgaacccg gtccccagcc ctacggaccc aggcatggtc aagcaaccgg
     2161 acacggtccc ccatcccgat tcagtcgtga cgcctccggt tgtggaccct aagatggcca
     2221 ttaacccgga agcggcaagc ccgcaaacgg aacctgctcc tcctccacgg gactcgtcgc
     2281 cgaagaaacc atgatcgatg agagcgaaag cactcgcctc agcgatgatg tcaaggaaat
     2341 gaccatcgca ggttgtcggg tgcgtctggc gtattgccgt gcgtctcacg agggatggat
     2401 cgtgaagggg acgttgcagt gcggactgga agatcaacaa ggcgagcgtt cgatcgtcac
     2461 ggaagcctat cccagccggg aagaagcgga gcagcacgca gtcgaacgag tcacggccct
     2521 gttgggtcaa cacaccgatc gcagccattc gcgcgttcgg aattggagct aactgcggtc
     2581 atagcattat tatccagacg gaggtcggca tggcaataat cggacaagcg attcccgacg
     2641 ggacctacca ggcctttcac gaagatgaaa ttcgcacaat caccttagac agctatcgcg
     2701 gccgatggtt ggtactcgta ttctatccgg gcgatttcac gtttatttgt cccactgaat
     2761 tagaagaatt aggcgcgctg tacggagact tcaaaaagct ggatgccgaa gtcatcggcg
     2821 tgagcacgga ttccgtctac gttcacaagg catggcatga cacatccccc gcgatcaaga
     2881 aactgccgtt cccccttctg tcggacgcgg gtggcgcctt gtccaacaaa ctgggggtct
     2941 atctttaccg ggaaggcgtc gccttgcgcg gtagctttat ttttgatccc gacggaaagc
     3001 tctgcgccta cgaagttcat gctaacaaca tcggacgaag catggaggag ctgttgcgca
     3061 agcttcaggc ggcgtctttc gtccatcaaa acggaggagc cgaagtgtgc ccggccggat
     3121 ggaaacctgg cgatcgaacc ctgcgtccca gcctcgatct ggttggcaaa ctgtaggctt
     3181 cgacgactat gacctttcaa tcgtggacat ggccgctgac tctcgttttc gcgttgatcg
     3241 tggcaacaac gagtgcctgc ggaacatcag ccacatctgt aggaaaaaac tcactcaaac
     3301 agcataccgg agagcccgga agtaccgtac caaggtccaa tccgccgcac tatcccaata
     3361 cctcgggcaa catcgtcgcg ccacccggca atcccggcgg taacccttcc ggttttcccc
     3421 ctatggtggg aggcacggaa actcagcaaa ccgcttggcc cgagggccga taaatatcat
     3481 ggcaaaggag cactcatgac tcgcacaacg atcatcaccg ccgccggggc attcatggta
     3541 ctgagcttca tgtctgcctg taatcgcgac caagaccaga caacgccagt cggacaacaa
     3601 caaggacaag gcagcgctca ggactatccg tccactcgca acatggaaca acccaatcct
     3661 gcgatcggcg ggaccaacag cgcaacgaac aagccggctc catccggaaa ccaggagccg
     3721 caagctggtt caactacgc
//
LOCUS       DASSCQ010000078        14036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_482084, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000078 DASSCQ010000000
VERSION     DASSCQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482084"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..74)
                     /locus_tag="VFH05_05320"
     tRNA            complement(3..74)
                     /locus_tag="VFH05_05320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Val,seq:gac)
     gene            complement(209..481)
                     /locus_tag="VFH05_05325"
     CDS             complement(209..481)
                     /locus_tag="VFH05_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6677665.1"
                     /translation="MTKEELIAKMAASAGITKVAAGNALHAFTGAVTSSLKKGQRVSL
                     VNFGTFTISKRKARMGRNPRTGESLKIPAAKVPKFSAGKVLKSAVR"
     gene            complement(571..1164)
                     /locus_tag="VFH05_05330"
     CDS             complement(571..1164)
                     /locus_tag="VFH05_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6677666.1"
                     /translation="MTLSSSVFSILLLSGDSEIQNHFRQTFKDAAMTTAKDATALPRD
                     AAKRAFDAVIIEAKPGMHNGSGGLPHHVDLSRTLVITGSKPVLKRASKFMQTIARAKG
                     TVSDGAPAPMSLEDYVEWKMGDFVKGMRNGSGRNLHPMLISAIERPLITKALQETKGN
                     QIQAAELLGLNRNTLRKKIQDLHIPVKRGRSARVREA"
     gene            1550..1987
                     /locus_tag="VFH05_05335"
     CDS             1550..1987
                     /locus_tag="VFH05_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET6677667.1"
                     /translation="MRVSLRSTYGIMAAVDMAMQAGSAPIQAKSIARRQGIPARFLEQ
                     VLHAMKKAGLVSSLRGAQGGYVLSKNPADLSVAEILEALEGPLFHSMNGRLGPRTKSD
                     PLLAKIWTRVSLAEREVLQGITVEELAGQQREIEEQRTLMYHI"
     gene            1996..4467
                     /locus_tag="VFH05_05340"
     CDS             1996..4467
                     /locus_tag="VFH05_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET6677668.1"
                     /translation="MSQLSTKSVAVKTQPIPPAIEEEIETFEQEAQRMLSGDIAGDLF
                     KPFRLQYGIYGQRQSGVQMVRIKIPFGGISANQLRRIAELSERYTTGVGHVTTRQDIQ
                     LHFAELKDIPAIMRGLAEVGLTTREACANTVRNVTGCHLAGVCQGEVFDITPYAKTVA
                     YHLLRNPLNQSLPRKFKIAFSGCKQDCALTPIHDIGLLAVKAEDGTIGFRIVVGGGLG
                     SAPRIAQVLREFTPMDEVIPTIEAVIKVFDTLGNRKNRNKARMKFVIEKLGFAEFKRR
                     WEEAYLSMGHARPTQQPIKLLPHQDEPVSLIMPTSNGVKSGVAQTNGHPNGVQQETPF
                     DMWKRTNVVRQKQAGYTAAVIKLFMGDLTASQMMHVADFAERYSNGNLRTTINQNLII
                     RWIHESRLADLYHDLASQGLSDPGAELVEDIIACPGTDTCGLGITSSKGLARALAEVF
                     PAGRVPDDLADVSVKISGCHNSCAQHHIATIGLHGVGKRIGEHTAPFYELHLGGKVNG
                     TAKIGQMTVKLPAKAVSAAITHLIDVYRRDRQTGERLPAFIDRLGKNALKDELIPYTI
                     VPPFAEDPTYYYDWEAEEEFVLEDLGPGECAGGALEMIENGILEADQELYQAKLLIDN
                     HQYSVSVNKSYRAVLAAAKALLVTEGLDPSTDADTFREFDGRIAQNGVVPAAYRDLVK
                     KVGDLGPKDTTAESAQEKMAFAKGFVDACRAATEQMGKDLKLTTDQATTASTFVEPKA
                     ATQVPTGAPVYDLRGVACPLNYVKTKLKLEMMDAGEKLEVWLDAGEPIKNVPLSLKND
                     GHLVLVQEALEPEAAHYRILVEKVE"
     gene            4513..5226
                     /locus_tag="VFH05_05345"
     CDS             4513..5226
                     /locus_tag="VFH05_05345"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET6677669.1"
                     /translation="MHTKQPTGDMLAWAESLEGSQPQEVLAAAIKRYAPRIVLACSFG
                     AEDVVLVDMIHRIDPSVGLFYLDTDFLFPETYATRDRIIERYQLKPAQVHQVKSLLTP
                     ERQAEQHGPALWTSDPDQCCKLRKVEPLTRVLSGFDAWVTGIRREQAPTRAHAKPIEW
                     DHIFQLVKVNPLVRWTWADVWTYISLYEVPYNEMHDRNYPSIGCIQCTKPVMPGDDPR
                     SGRWEGRDKTECGLHKTSS"
     gene            5223..6278
                     /gene="trpD"
                     /locus_tag="VFH05_05350"
     CDS             5223..6278
                     /gene="trpD"
                     /locus_tag="VFH05_05350"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET6677670.1"
                     /translation="MMGMQELIAKIAKGPKASKDLTWDEAKRAIHALIEGDATPAQAG
                     AFLIAMRFKMESVTELAAFTAATRSYVAPLPIPQELGVVDLPSYAGKQDTFHALAGAA
                     IVAAAAGAAILMHGYDGVPGRPGNAGLLKALGIPIDLESKAVAALVSKQGFAYLDIAL
                     YHPPLYRFLEMRQELGVRNVFHPIARLLNPARAASQVIGLTHPPHFEKTAEAVRMLGM
                     PRALVIRGVEGDPELSIAMATKVLELRDERIAPLTLAPKDAGLPLGTSREMAGFSNEH
                     REKEADLLRRILHNQISGGQRDWVLLNAGLLLYAAGKRASLGEGVSLARRTLEDGTAA
                     RKLESLSHATMAAAQQT"
     gene            6296..7093
                     /gene="cysD"
                     /locus_tag="VFH05_05355"
     CDS             6296..7093
                     /gene="cysD"
                     /locus_tag="VFH05_05355"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET6677671.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFDNLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSFKIPAMIEYRDRLAREWHLNLVVGQNKDALAAGMNHTLGRVT
                     CCTALKTNGLKQLLEQKGYTGVILGVRADEEGTRAKERYFSPRDKHGDWDFRDQPPEL
                     WDQFKTTFPPGTHIRIHPLLDWTEINIWEYIKLENIPFIDLYLNKGDGTRYRSLGCAP
                     CTTPIKSTAKTVDEIIEELRHTTVAERSGRAQDEGRGMELLRKDGYM"
     gene            7153..8991
                     /locus_tag="VFH05_05360"
     CDS             7153..8991
                     /locus_tag="VFH05_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET6677672.1"
                     /translation="MGTAKSSENLNIVIVGHVDHGKSTLLGRLYADTGSLPDGKLEKV
                     QAICRQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFMWKGRQYIIIDAPGHKEFLKN
                     MISGAARAEAALLLIDALEGVKEQSKKHGYLLSLLGVRQFAVVVNKMDLVGYRQDVFE
                     GIEKEYREFLAQFGAVPERIIPVSAKIGDNIANRSGTMPWYQGPTVLDTLSLFKKETA
                     RPEQPLRFPVQDVYKFDARRIIAGRITAGRLKIGDHLVFSPSNKRANIRSIEAFNVEP
                     VLTGAEAGQSVGITLDEQIFVERGEVATHQEQLPLVSTAFKANLFWLGKQPLEQGRRY
                     LLRVATREVECEVAAIHRIIDTMDLNQQSGSNKVARNQVGELTLRTKAPVAFDLSASF
                     EATGRFVLVDDYDIAGGGIVTEMIRDDQEFLREEARRRDFAWVKGEVGIEERAQQYGH
                     RAAIVLITGGRHTGKSFLAKKLEARLVADGRHAYLLDGENLRRGLDADLAEGERSQTM
                     EMARRYGEVARLLADTGLIVVSTTNPFGMAYAEAAQAIRTLVHPTPVISIQMSTLSDE
                     ASTNADLVFSGPADFDAATKSILDELKRRGVLAQAIGAKPTFQYSI"
     gene            9112..10032
                     /locus_tag="VFH05_05365"
     CDS             9112..10032
                     /locus_tag="VFH05_05365"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET6677673.1"
                     /translation="MAVEKDLKSILGKLQYTDDAKVVAQITDQMKQVQARMAGIKHKL
                     VVMSGKGGVGKSMTTVNLALALARQHARVGLLDVDLNGPCVPRMLGLHGRSLAITPEG
                     AVPPVGPLGIKVASMDFFLGTSSPVRWKGPMDVSPVWLGLMEMNVIREFLADVVWGEL
                     DYLLADLPPGAAADKPPVIAGFIPDLAGAIVVTTPSEVASDVVQKSVTYARDMGIKVL
                     GIVENMSEYRCPSCGELNELFEGNTDAMCEALDLPLLGRIPFDRKLARTFDKGEPLLD
                     ETYPTIQRYQDIAGRIRTLLDYKRVLAEKL"
     gene            10049..10798
                     /locus_tag="VFH05_05370"
     CDS             10049..10798
                     /locus_tag="VFH05_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HET6677674.1"
                     /translation="MKFVCLTCETYMNFEKVEKPGEGSLGVFFGCPSCGAKFSMVTNP
                     GETQMVSSLGVQLGGRTAQAQPFEMTRGTLKEEAQAGSGQMAAYLNDRIQGGQPLTAS
                     DHSPKSGEKSSGGCPFSAMVAEMGLTSGGKPANGGVTKSDFTWTPDAKEKLDRLPAFV
                     KPMVQGSVEAFARKHGYETITLQVMDDSKNDSPNGMSWTPEAQQRLDNIPDFIRPMAR
                     KEIERLAKERGVATITAEVMDDAKDKFMKFM"
     gene            10885..11649
                     /locus_tag="VFH05_05375"
     CDS             10885..11649
                     /locus_tag="VFH05_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677675.1"
                     /translation="MKVDCILRIVRRCVLTSGFLILVLLAPAAAQQSSSLVVDSLHAG
                     HHGQHGRPAPEAWEGSAKGIAYSERNHHIAGLLVILMGVAELTHAMRLAALSWVRLLL
                     PSALLCAGAFLLIWSDHEAWPIGSLTFGQSFFGDDYEIIQHKVYGILAFAVGSVELFR
                     RMGRAGHRAWASPLPLMAIVGGLMLFGHSHGFHPSAQKIATHHAIMGTLALTAGSSKL
                     FSTWFNPTPGAAAVRWDWIWAFFILLIGTQLLIYSE"
     gene            11789..12547
                     /locus_tag="VFH05_05380"
     CDS             11789..12547
                     /locus_tag="VFH05_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6677676.1"
                     /translation="MGGHSHWATIKRHKGAQDAKRGKIFTRIIREVTIAARAGGDPDG
                     NPRLRLAIAKAKEANMPGDTLKKAIQRGTGELPGVTYEEFSLEGYGPGGTALLIEITS
                     DNRNRTVAEIRSVLTKNHGNMAEAGAVSWQFQKKGLLTIDKSKSDEDTLLSLALEAGA
                     EDVKVGEKTFEVITNPQDFDAVKKSLADAKIESSMAEITYIPQNTIRLEEKGAEQMLK
                     LMEILDEHDDVQKVHANFDIPEDVMEKVAAATAG"
     gene            12569..13177
                     /gene="ruvA"
                     /locus_tag="VFH05_05385"
     CDS             12569..13177
                     /gene="ruvA"
                     /locus_tag="VFH05_05385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6677677.1"
                     /translation="MIAHLTGRLAFKAPTHLALDVHGVGYEVFIPLSTYYNLPNADES
                     LTLSIHTHVREDAIQLFGFSTRQEKDAFVLLMSVSGIGPKSALGILSALPVADLASAI
                     QSGDIEKLETVPGIGKKSAGRLVLELKDKLTRFHPGMTPAVDQSTAGQDDVFDDALSA
                     LTNLGYRSTDAKDALKTVRKSRPQPLSLQELIRETLKHLARV"
     gene            13180..13680
                     /locus_tag="VFH05_05390"
     CDS             13180..13680
                     /locus_tag="VFH05_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677678.1"
                     /translation="MLITVQRTNLLTASSITLVLIGLLQPALAPVAWAGDASAELKSI
                     RKTCGKCPDGYATTGVTEAPELCKGGDPTLTQCVPLGVNMLPVCGSCPDGYREVGHSS
                     VPARCGSQDGGRLAQCQLETMEQHLPDPAQGGKICPPDCGSTPPPGRGTLPPPPKYVP
                     APDTPH"
     gene            13705..>14036
                     /locus_tag="VFH05_05395"
     CDS             13705..>14036
                     /locus_tag="VFH05_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6677679.1"
                     /translation="MTRVVNAQLTDEERSLEQHLRPQTLNEYIGQARMKDSLRICIEA
                     AKQRREPLDHAIFYGPPGLGKTTIAHIIAREMGGTFCATSGLVLTHAGDLAAILTNLQ
                     EHDVLFID"
BASE COUNT         3154 a         3803 c         4135 g         2944 t
ORIGIN      
        1 ggtaggcgcg gacggtcttg aaccgtcgac ctctaccgtg tcaaggtagc gctctccccc
       61 tgagctacgc gcctatcgtc cgagcgcgca tgctaatata gccccgccca aggtgtcaag
      121 aaatgcaaag agaagggagc gtgctccgat gtgagcacgc cgccttctct cttttccgcg
      181 atccggcgcg taaggggtga tccggaggtt accggacagc cgacttcaaa actttccctg
      241 ctgagaactt cggcactttt gcagcgggaa ttttgaggct ctccccggtc cgcggattcc
      301 gtcccatcct ggccttgcgc ttggaaatcg tgaaggtccc gaagttgacc agcgacactc
      361 gctgtccttt tttcagagaa gaggtcacgg ctccggtaaa agcatggagc gcatttccag
      421 cggccacctt ggtaatcccg gcagacgctg ccatctttgc aatcagttct tctttcgtca
      481 tcatcgccct ccttgatatg ggttaaaaac cgcctccacc acttgctgcc ttgcaggctt
      541 ctctcctgct gtatccgagg tccggaacga ctaggcctcc cgtacacgcg ctgaccgtcc
      601 tcgtttgacg ggaatgtgca gatcctggat ctttttcctg agggtgttgc ggttcaagcc
      661 aagcagctcg gcagcctgga tttggttgcc tttagtttcc tgcaaggcct tggtgatgag
      721 gggccgttca atagcggaaa tcagcatcgg atgaagattg cgtcccgagc cgtttcgcat
      781 gcctttgaca aagtctccca tcttccattc cacatagtct tcaaggctca tgggcgcggg
      841 cgcgccgtca gacaccgttc ctttggcgcg ggcaatcgtc tgcatgaact tcgaggcccg
      901 cttcagcacc ggcttggacc cggtgatgac aagagtccgg gacaggtcga cgtggtgagg
      961 cagtccgccc gatccattgt gcatgccggg cttggcttca ataatcacgg cgtcgaatgc
     1021 tcgcttcgcc gcgtctctcg gcaatgccgt cgcatctttg gcggtagtca tcgccgcatc
     1081 tttaaatgtt tgcctgaagt ggttttggat ttccgaatcg ccgctgagca acaggatgct
     1141 gaagaccgaa gaagagagcg tcatgaagcc atcaccagaa gaagagggcc ggattattgc
     1201 ccagagccga tttgatgtca atgaagaaat gtgaggacga acggatttat tacagggaac
     1261 ggtgtgctgc aaaagaatct ttcacgccgg atcgaagccg gctctgtcgt ggatcgactg
     1321 aacgaacatc tggtgcagga tgatgatgtt cctcgagtac tgtccgacag gacgcctcgt
     1381 cttccagccg aagcatgagg attctgtgat cgaggatgat ggatgagtgc gtattagctt
     1441 tcgcagagaa aagcctatga ttgagggggg cggttgccac accttgacag gatgagaagg
     1501 gccagagtat aactttacat tcatctaatt ccgagcggaa tagtgaggta tgagggtatc
     1561 cctgagatct acttatggaa ttatggctgc cgtggacatg gccatgcagg ccggttccgc
     1621 tcccattcag gctaaatcta tcgccaggag acaggggata ccagcaagat tccttgagca
     1681 agtgctccac gcgatgaaaa aggccggctt ggtgtcgagt cttcgagggg cccaaggcgg
     1741 ctatgttctg tcaaagaatc ccgcagatct gtccgtcgcc gaaattttgg aggcgctcga
     1801 aggtcctcta tttcacagca tgaatggacg actgggtccg aggacaaaat cagatccgct
     1861 cctggccaag atatggactc gcgtgagtct agcggaacgc gaggtccttc aagggatcac
     1921 cgtcgaagag ctcgctggac aacagcgcga aatcgaagag caacgtactc tcatgtatca
     1981 catttgaggt cagccatgag ccagctctcc accaaatcgg tagccgtcaa gacgcagcca
     2041 attcctccgg ccattgagga ggagatcgag accttcgagc aggaggcgca acgcatgctg
     2101 tccggcgata tcgccggcga tctctttaag ccgttcaggc ttcagtacgg aatctacggt
     2161 caacggcagt ctggcgtaca aatggtgcgc atcaagattc ctttcggcgg tatttcggcc
     2221 aaccaactca gacggatcgc cgagctgtcg gagcgctata cgaccggcgt gggccatgtg
     2281 acgacccgcc aagacatcca actgcatttt gcagagctga aagacatacc cgccatcatg
     2341 cgaggtctgg ctgaagtagg cttgacgacc agagaagcct gcgccaacac agtccgaaac
     2401 gtcactggtt gccatttggc gggggtctgc cagggcgagg tgtttgacat taccccttat
     2461 gccaagacgg tggcctatca tcttctgcgg aacccgctta atcaaagcct gccccgcaag
     2521 tttaaaattg ccttttccgg gtgcaaacag gactgcgcgc tgactcctat tcacgatatc
     2581 ggactgcttg ccgtaaaagc cgaggacggc accattggtt tccgtattgt tgtcggaggc
     2641 ggcctgggtt ctgcgccccg cattgcgcag gtgctgcgtg aatttacacc gatggacgaa
     2701 gtgatcccga cgatagaagc cgtcattaag gtgttcgaca ccctgggcaa cagaaaaaat
     2761 cgcaacaagg cgcgaatgaa gttcgtcatt gaaaaactgg gattcgcgga attcaaacgg
     2821 cgttgggaag aggcgtatct ctcaatgggc cacgcgaggc cgacgcagca gccgatcaag
     2881 ctgctcccgc atcaggatga gcctgtttcg ttgattatgc cgacgtccaa tggggtgaag
     2941 tccggcgtcg cgcagaccaa cggtcatccg aacggcgtcc agcaggagac gccctttgac
     3001 atgtggaagc ggacaaacgt cgtacgtcag aagcaagccg gttatacggc ggctgtcatt
     3061 aaattgttca tgggagacct gaccgcaagt caaatgatgc atgtggcaga cttcgccgag
     3121 cgctattcaa acggtaacct ccggacgaca atcaatcaga acctgatcat ccgatggatt
     3181 cacgaatccc gtcttgccga tctttatcac gacctcgcat cacaggggct gtcggacccc
     3241 ggcgccgaac tcgtcgaaga catcatcgcc tgtcccggca ccgatacctg cggtcttggt
     3301 attacctcgt ccaaggggct ggcacgagcc ctggcggagg tgtttcccgc cggacgtgtg
     3361 cccgatgatc tcgcggacgt gagtgtcaag atcagcggat gccacaattc ctgcgcacag
     3421 caccatatcg cgacaatcgg cttgcacggt gtcgggaagc gtatcggcga gcatacggcg
     3481 ccgttctacg agcttcatct tggcgggaag gtgaatggca cggctaaaat cggtcagatg
     3541 acggtcaagc tgcccgcgaa agctgtatcg gcagccatta cgcacctcat cgacgtctat
     3601 cggcgggacc gtcaaaccgg tgaaaggctc ccagccttca tagaccgact ggggaaaaat
     3661 gcactgaaag atgaactgat tccttatacg attgtgccgc cgtttgccga ggatcccacc
     3721 tattattacg actgggaggc ggaagaagag tttgtcctgg aagatcttgg gcccggtgaa
     3781 tgtgccggcg gcgcgctgga gatgatcgag aatggaatct tggaagccga tcaagaactg
     3841 tatcaggcaa agctgttgat agacaaccat caatactccg tttcggtgaa caagtcgtat
     3901 cgcgcggtcc tcgcggcagc gaaagcgctg ttagtgacgg aaggtctcga tccctctacg
     3961 gacgccgata cgttccgaga gttcgacggc cgcatcgctc aaaacggcgt ggtgccggct
     4021 gcgtatcgtg acctcgtcaa aaaggtgggg gacctggggc cgaaggatac gacggcagaa
     4081 tcggctcaag agaaaatggc cttcgcgaaa ggctttgttg atgcgtgccg ggccgccaca
     4141 gagcagatgg ggaaggatct caagctcaca accgaccaag ccactacggc ctctacgttc
     4201 gttgagccga aggcggccac acaggttccc accggtgctc cggtctatga tctcaggggt
     4261 gtcgcgtgcc ccctcaatta tgtcaaaacg aagttgaagc tggagatgat ggatgccggt
     4321 gaaaaattgg aagtgtggtt ggacgcaggg gagccgatca agaacgtgcc cttgagtttg
     4381 aagaatgacg gacatctcgt actcgtacag gaagcgctgg aaccggaagc ggcgcactac
     4441 cgaattctgg tcgaaaaagt ggaatgagtt gagcgcgcat ctcagtgtgg caaggatagg
     4501 gtggaaaaca acgtgcacac aaagcaacca actggcgaca tgctggcctg ggccgagtcg
     4561 ctggaaggca gccaaccaca agaggtgctc gccgccgcca tcaagcgcta cgctccgcga
     4621 attgtgctcg cctgcagctt cggtgcggag gacgtggtct tagtggacat gatccatcgg
     4681 attgacccgt cggtggggct gttctatctc gatacagatt ttctcttccc cgagacgtac
     4741 gcgacgcgcg accgaatcat tgagcgatac cagctgaaac cggcccaagt gcatcaagtg
     4801 aagtctcttc tgaccccgga acggcaggcg gaacagcatg ggcctgccct gtggacgagc
     4861 gaccccgacc agtgctgcaa gttgagaaaa gtcgaaccgt tgacgcgtgt gctgagtgga
     4921 ttcgatgcgt gggttaccgg tattcgacgg gaacaagcgc cgaccagggc ccatgccaag
     4981 ccaatcgagt gggatcacat ctttcaactc gtcaaggtga atccgctcgt ccggtggacg
     5041 tgggccgatg tctggactta catcagcctg tacgaagtgc cttataacga aatgcatgac
     5101 cgcaactatc ccagcatcgg ctgtattcag tgcacgaagc cggtcatgcc cggtgacgat
     5161 ccgcggtccg gacggtggga aggccgggat aagaccgaat gcggccttca taagacatca
     5221 tcatgatggg catgcaagaa ctcattgcca aaatcgccaa gggacccaag gcttccaagg
     5281 atctcacgtg ggacgaagcc aagcgggcca ttcacgcctt gatagaaggc gacgccacgc
     5341 ccgcgcaggc cggggcattt ctcattgcca tgcgtttcaa gatggagtcc gtgacagagt
     5401 tggccgcctt cacggccgcg acccgctcct atgtggcgcc gcttccaatc cctcaggaac
     5461 tcggtgtcgt ggatctgccg tcatatgccg gtaagcagga caccttccat gcactggccg
     5521 gcgccgcgat tgtggcagct gcggccggtg ctgccatact catgcatgga tacgatggcg
     5581 ttccaggtcg tccgggaaat gccgggctgc tgaaggcctt gggaattccc attgaccttg
     5641 agtccaaagc cgtcgcggca ctcgtatcga aacaggggtt cgcctacctc gacattgcgc
     5701 tctatcaccc cccgctctac cggtttctgg aaatgaggca ggaactgggg gtgcggaacg
     5761 tgttccatcc cattgcgcgg ctcttgaatc cggcacgcgc tgcctcgcaa gttatcggcc
     5821 tcactcatcc acctcatttc gagaagacgg ccgaggccgt tcggatgttg ggcatgccga
     5881 gagcgcttgt gatacggggc gtcgagggcg atcccgagct gtcgatcgcc atggcgacca
     5941 aggtgttgga gttgcgggac gaacggattg caccgttgac cttggcgccc aaagacgccg
     6001 gattgccatt gggaacgtcc agagagatgg ctggcttttc taacgaacat cgagaaaaag
     6061 aggcggatct gctcagacgg attcttcata accagatttc cggaggccag cgggactggg
     6121 tactgctcaa cgccggtctg ttgctctatg cggcagggaa acgggcatcg ttaggagaag
     6181 gggtatcgtt ggcacgacgg acgcttgagg atggaactgc cgcgagaaaa ctggaaagcc
     6241 tgagtcacgc aaccatggct gctgctcaac agacctagag ggaagaggtc catcagtgaa
     6301 acacctgcgc caattggaag accagagcgt atacattttg agggaagcct acaagcattt
     6361 cgacaatctc gccatgctat ggtcgatggg gaaggattca acggtgctgt tgtggctggc
     6421 tcgaaaggcg ttttttgggc atgtgccatt cccgttgctg catgtcgaca cgagtttcaa
     6481 aattcccgcc atgatcgaat accgagacag gcttgcaagg gaatggcatc tgaatctcgt
     6541 cgtcggccaa aacaaagacg cgttggccgc cggtatgaac catacgttgg gccgcgtcac
     6601 ctgttgcacg gcgctgaaaa ccaacgggct caaacagttg ctggaacaga agggctacac
     6661 cggagtcatc ttgggggttc gggcggacga ggaaggcacc agggcgaaag aacgctattt
     6721 ttccccgcgc gataagcatg gcgactggga ttttcgtgat caacctccgg agctctggga
     6781 tcaattcaag actacgtttc ctcccgggac acacatcagg atccatccgt tgctggattg
     6841 gacggaaatc aatatctggg agtacatcaa gctcgagaat attcctttca tcgacctcta
     6901 cctgaacaag ggagatggga ctcgttatcg cagcctgggc tgtgctccct gcaccacccc
     6961 gatcaaatcc acggcgaaaa cggtcgatga gattatcgaa gagctgcgtc atacgaccgt
     7021 ggccgagcgc tccgggcgtg cgcaggatga aggccgtggg atggagttgt tacgaaaaga
     7081 tggatacatg tagtgccgag gactgccgct gcggttgctg ggtgggaatg gctgaacaag
     7141 tcgaggagca ttatgggtac ggcgaaatcg tccgagaacc tcaatatcgt catcgtcggg
     7201 cacgttgacc acggcaagtc gacgctgctc ggacggctct atgccgatac cggctctcta
     7261 ccagacggca agttggaaaa ggtgcaagcc atctgccggc agcagggaaa ggaattcgaa
     7321 tatgcctttt tgttcgatgc ctttctggaa gagcaggagc aaggcatcac gattgacacc
     7381 gcacggacgt tcttcatgtg gaaaggccgg caatacatca ttatcgatgc gccaggccac
     7441 aaggaatttc tcaaaaatat gatctccggg gcggcaaggg cggaggccgc gctgctgctg
     7501 atcgacgcgt tggagggagt caaggagcaa tccaaaaagc acggctacct cttgtctctc
     7561 ttgggcgtcc ggcaatttgc ggttgtggtc aacaagatgg atctcgttgg ataccgccag
     7621 gatgttttcg aaggtatcga gaaagagtat cgcgaatttc tggctcagtt cggtgcggtg
     7681 cccgagcgca tcattcccgt gagtgcaaaa atcggtgata acatcgccaa ccggagcggc
     7741 accatgccct ggtatcaggg gccaacggta ttagacacac tgagtctctt caagaaggag
     7801 acggcccgac cggagcagcc gctgcggttt ccggtgcagg acgtctacaa attcgatgcc
     7861 cgccggatca tcgccggccg cattacggcc ggacggctca agatcggcga ccatctcgta
     7921 ttttcaccgt caaataaacg agccaacatc cgctcgatcg aagcgtttaa tgtcgaaccg
     7981 gttctcaccg gagccgaggc gggacagtct gttggcatca ctcttgacga acagatattt
     8041 gtcgagcgag gagaagttgc cacgcatcag gagcagttgc cgctggtatc gacggcattt
     8101 aaggccaacc tcttttggct ggggaagcag ccgctggaac aaggccgcag gtatctcctg
     8161 cgagtggcca cgagggaagt cgagtgcgag gtggcggcca tccaccggat catcgacacc
     8221 atggacttga atcagcagtc aggcagcaac aaggtagctc gtaaccaagt cggagaattg
     8281 accttgcgga ccaaggctcc ggtcgcattc gacttgtctg cctcattcga agcgacaggt
     8341 cgttttgttc tggttgacga ttatgatatt gccggcggcg ggatcgtgac ggagatgatc
     8401 agagacgatc aggaatttct ccgggaagag gcgcgccggc gagacttcgc gtgggtcaag
     8461 ggtgaagtcg gcatcgagga acgggctcag caatatggcc atcgtgccgc aatcgtgtta
     8521 atcaccgggg gaagacatac ggggaagtct tttttggcca aaaaactcga agcaaggctc
     8581 gtcgccgacg gacgccatgc gtatctgctc gacggagaga atctgcgcag gggtttggat
     8641 gccgatcttg ccgaggggga acggagccag accatggaga tggcccgacg ctatggtgaa
     8701 gtcgcgcgat tgctcgctga tacggggttg attgtggtct cgacgaccaa tccgttcggc
     8761 atggcctatg ccgaggcagc gcaagccatc agaacattgg tccatccgac gccagtgatt
     8821 tcgattcaga tgagcacgct ttccgacgag gcttcgacaa atgccgatct ggtgttctct
     8881 ggtccagcgg attttgacgc cgccacaaaa tctatcctgg acgagctcaa gcgccggggt
     8941 gtgttggctc aagcgattgg ggccaaaccc accttccagt attccatcta gcagcttgcc
     9001 gaaccggccg ccactgagca atgtcaggag cctggttcct ggtttgactg gcttaaaact
     9061 gctgtgttac cgtaccaccc ttatggtttt ctcttgcagt gaaggcatct tgtggccgta
     9121 gaaaaagatc tcaaatccat cctcggtaaa ctccagtata ccgacgacgc caaagtcgtt
     9181 gcgcaaatca ctgatcaaat gaagcaggtg caggctcgca tggccggcat caagcataaa
     9241 ctggtcgtca tgagcggcaa gggcggcgtc ggaaagagca tgacgaccgt gaacctcgcc
     9301 ctcgccctgg cccgccagca cgcgcgagtc gggctcttgg acgtcgatct caatggcccc
     9361 tgtgttcccc ggatgttggg ccttcatggg cgatcgttgg cgatcacgcc ggagggcgcc
     9421 gttccgccgg tcgggccact cgggataaaa gtggcttcca tggatttctt tctggggacg
     9481 tcatcgccgg tccgctggaa gggtcctatg gatgtcagcc cggtctggtt gggcttgatg
     9541 gagatgaacg tgattcgcga gtttctggct gacgtcgtat ggggagaact ggattacttg
     9601 ctggccgact tgcctcccgg cgccgcggcc gacaagcccc cggtcatcgc tggtttcatt
     9661 cccgatttgg caggtgcaat tgttgtgacg acgccttctg aagtggcatc cgatgtcgtg
     9721 cagaaatccg tcacgtacgc ccgggacatg ggcatcaagg tgttggggat cgttgaaaat
     9781 atgagcgaat accggtgccc ctcatgtggc gaactcaacg agttgttcga aggcaacacc
     9841 gacgccatgt gcgaagcgct ggatctccct ttattgggcc gcattccctt tgatcgcaag
     9901 ctcgcccgta cgtttgacaa gggtgagcct ctgctcgatg agacctaccc gaccatccag
     9961 cgctatcagg acatcgccgg gcggatcagg actctattgg attacaagag agtgttggcg
    10021 gaaaaattgt gagctgagag gagctgtcat gaaatttgtg tgtctcacct gcgaaacata
    10081 tatgaacttc gagaaggtcg agaagcctgg cgaaggatcg ctcggcgtct tcttcggttg
    10141 cccgtcgtgt ggtgcgaagt tctcgatggt gaccaatccc ggtgaaacgc agatggtcag
    10201 ctcgcttggg gtccagttgg gtggacggac tgcacaggct cagccctttg aaatgacccg
    10261 aggaacgctg aaggaagaag cgcaagccgg gtccggtcag atggcggcct atttgaacga
    10321 cagaattcaa ggcggtcagc ccctcacggc gtctgaccat tcgccaaaat ccggcgagaa
    10381 aagcagcggg ggttgcccct tctcggctat ggtggcggaa atgggtttga cctcaggcgg
    10441 caagccggcc aatggtggtg tgaccaagtc tgattttacc tggacgccgg atgccaaaga
    10501 gaaactggat cggcttcccg ctttcgtcaa gccgatggtg cagggcagtg tcgaggcctt
    10561 cgcccgtaag cacggctatg aaacgattac tcttcaagtg atggacgatt caaagaacga
    10621 ttcgccaaac ggcatgtcgt ggacgcccga agcgcagcaa cggctcgaca acattcctga
    10681 ctttatccgt cctatggcgc gaaaggaaat tgaacggctg gctaaagaac gcggtgttgc
    10741 gactatcacc gccgaagtca tggacgatgc caaggacaag ttcatgaaat tcatgtaaca
    10801 cggatattgc cccggtcttg aaacatgagg cccatccggg gaaccggatg ggcctttgac
    10861 gtgagacgct tctgatatgc ctacatgaag gtcgactgta tcctccgcat cgtgcgacgc
    10921 tgcgtgttga cgagtgggtt cttgatcctg gtcttactgg cgcctgccgc tgctcaacag
    10981 tcatcatcgc ttgtggttga ctcgctgcac gctgggcatc atgggcagca cggtcgccct
    11041 gcgcccgagg cttgggaggg ttcggccaaa ggcatcgcct attcagagcg caatcatcac
    11101 atagccggtc ttctggtgat cttgatgggc gtcgctgagc tcacccatgc catgcggctc
    11161 gccgccttga gctgggtccg gcttctgttg ccctccgccc tcttatgtgc tggagcgttc
    11221 cttctgatat ggagcgatca tgaagcgtgg cccatcggct cgctgacctt cggccaatcg
    11281 ttctttggcg acgactacga gattattcag cacaaggttt atgggattct ggcgtttgca
    11341 gtcgggagcg tggagttgtt tcgacgaatg ggccgtgccg gccatagagc gtgggcatcg
    11401 ccgcttcctc tcatggccat cgtgggaggc ctgatgctgt tcggccactc acacgggttc
    11461 catccttctg cacagaagat tgcgacgcat catgcgatca tgggaacgct ggccctcacc
    11521 gcagggtcgt caaagctgtt ctccacctgg ttcaatccga cgcccggagc agccgcggtc
    11581 agatgggatt ggatttgggc gtttttcatc ctcctgatcg gaacacagct cctcatctat
    11641 tccgaataag agtccttcta ttccgaataa gtgctcttct tcgtcgcccg cactgtaatt
    11701 gacacccctc caaaccctgt gttaggtcta caacttcacc catgtaattg gaaagccggt
    11761 gcgccttggg cggccgaatg gagcagttat gggcggacat agtcactggg cgacgatcaa
    11821 acgacataaa ggagcccaag acgccaaacg ggggaagatc tttacgagga tcatccgcga
    11881 agtaacgatc gcggcccgtg caggcggaga tccggacggc aatcccaggt tgcggctggc
    11941 tattgccaag gccaaggaag ccaatatgcc cggtgacacc ttgaagaagg cgattcagcg
    12001 cggcacaggc gagttgcctg gtgtgactta tgaggaattt tcgctcgaag gctatggtcc
    12061 gggcggaacg gcgctgttga tcgagatcac cagcgacaac cggaatcgga ccgtggcgga
    12121 aatccgcagt gtcctcacga aaaatcatgg caacatggcc gaagccggag ccgtttcctg
    12181 gcagtttcag aaaaagggat tgctgacgat cgacaagtct aagagtgacg aagacaccct
    12241 tctttctctt gcactcgagg ctggagcaga ggatgtgaaa gtcggggaga aaacattcga
    12301 agtcatcacc aatccgcaag acttcgacgc ggtgaagaaa tccctggcgg atgcgaagat
    12361 tgagtcgtcc atggccgaga ttacctatat accccaaaat acgatccggc tggaggagaa
    12421 gggcgccgag caaatgttga aactcatgga gatcctggac gagcatgacg acgttcaaaa
    12481 ggtgcatgcc aatttcgata tccctgaaga tgtgatggaa aaggtcgcgg ctgcgacagc
    12541 cggctagtta ctcatccgcc accgacgcat gatcgcccac ctgacaggcc gactggcctt
    12601 caaggcgccc acgcatctgg ccctcgatgt ccacggggtt ggatacgagg tgtttattcc
    12661 cctcagcacc tactataacc ttccgaatgc ggacgagagt ctcaccctca gtatccatac
    12721 ccacgtcagg gaagatgcca tccaattgtt cggatttagt acccgccagg agaaagatgc
    12781 ctttgtgctg ctcatgagtg tctccgggat cgggccgaag tcagcgctgg gcatcttgtc
    12841 cgccttaccc gtcgccgatc tagcctctgc aatccagtcg ggtgatattg agaaattaga
    12901 aacggttcct gggatcggga agaaatcagc cggtcgactg gtactggagc tgaaagataa
    12961 attgacaaga ttccacccgg gaatgactcc ggccgttgac cagagtaccg ccgggcagga
    13021 tgatgtcttt gacgacgccc tttctgccct cacgaatctc gggtatcggt caacggacgc
    13081 aaaagacgca ctcaaaacgg ttcggaagtc acgccctcag ccattgagtc tacaggagct
    13141 cattcgcgag acattgaaac atctggccag ggtatgagca tgctcatcac cgtacagagg
    13201 acgaacctct tgaccgcctc atcgatcacg ctggtactga tcggccttct ccagcctgcg
    13261 ctggctcccg tcgcgtgggc gggcgacgcc tctgcagagc tgaaaagcat ccgcaagacc
    13321 tgtggaaaat gtcccgatgg atatgccaca accggcgtga ccgaggcgcc tgaactatgc
    13381 aaaggcggcg atcccacctt gacgcagtgt gttcccctgg gtgtgaacat gctgccggtt
    13441 tgcggatcct gtcccgacgg gtatcgagaa gtcggccatt catccgtgcc ggcacgttgt
    13501 ggaagccagg acggaggacg gcttgcgcag tgtcaattgg agaccatgga acaacatctc
    13561 cccgatccgg cgcagggtgg caaaatctgc cctccggatt gcggcagcac gccgcctccc
    13621 gggcgaggca cgctcccacc tccacctaaa tacgtcccgg cgcccgatac accgcattaa
    13681 gattgacctg gagattcgcg gattatgact cgtgtcgtca atgcccagct gacagatgaa
    13741 gaacggagct tggagcagca cctccgtccg cagaccctga acgagtacat tgggcaggcc
    13801 cgaatgaaag attcgctacg aatctgcatc gaggcggcaa agcagcgacg agagcctctg
    13861 gaccatgcga ttttctatgg tccgccgggc ctgggaaaga cgaccattgc tcacatcatt
    13921 gcacgtgaaa tgggcgggac gttttgcgcc acctcggggc ttgtattaac gcatgccggt
    13981 gatctggctg ctatcttgac gaacttacag gaacacgacg tcctcttcat tgatga
//
LOCUS       DASSCQ010000079         4584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1285135, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000079 DASSCQ010000000
VERSION     DASSCQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285135"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFH05_05400"
     CDS             complement(<1..441)
                     /locus_tag="VFH05_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6677680.1"
                     /translation="MAKKQRRLRANKAKTDHKDSDENGGGQRRTLVVDVGGSGIKATL
                     LNDLGKAVGDRLRRDSPSSGMPGEVMDIIVSLSKQFESFDRVAIGFPGVVINGIVKQA
                     PNFAKEWQDYNIAEVLEARLKKPVRVANDADVQGFGAISGTGVEL"
     gene            complement(910..1245)
                     /locus_tag="VFH05_05405"
     CDS             complement(910..1245)
                     /locus_tag="VFH05_05405"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6677681.1"
                     /translation="MIECRQYPRVPVELQVYFSTSGNTSISEGTMFDLSAGGCAVTSM
                     SAVRPGSAVRILIRATDLGSPITIESAAVRWSKHGEFGVEFLGLSELDQNRLHRLLHA
                     KTSYPLRPS"
     gene            complement(1414..3108)
                     /locus_tag="VFH05_05410"
     CDS             complement(1414..3108)
                     /locus_tag="VFH05_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET6677682.1"
                     /translation="MTAGTTASNFIREIVSADRAAGKHGGRVVTRFPPEPNGYLHIGH
                     AKSICLNFGLAQDPPGGKCHLRFDDTNPTTEDPEYVQAIQEDIRWLGFDWGENLFFAS
                     DYFERLYRLAEDLIQKGLAYVDSLSPDDIRRYRGTLTEPGTNSPYRDRPAEESLDLFR
                     RMRAEEFPDGTHVLRAKINMASPNMNLRDPILYRIRHAAHYRTGKTWCIYPAYDYAHP
                     LSDAFEGVTHSICTLEFEDHRPLYDWVVEQAGTAACPQQIEFARLNISQAVMSKRKLL
                     ELVEKKLVAGWDDPRLPTIKGLRRRGYTPEAIRAFCDHIGVSKRDSVVEMQLLEHFIR
                     EDLNKRAPRVMTVLKPLKVIIDNYPDGVTEQLEAINNPEDPSAGTRPIPFSRTLYIER
                     DDFLEDPPKQFFRLSPGREVRLRYAYIIKCVGVMKDAVTGEITELHCTYDVETKSGAP
                     SAQRKVKATIHWVSAAHAVEAEVRLYNPLLKGDLSGVVDQQDWTAHLNPQSLERLLGC
                     RAEPSLKEAQPGSRFQFERLGYFCMDPDSLSDRLVFNRTVSLKDAWAKLEKSGASR"
     gene            complement(3234..3647)
                     /locus_tag="VFH05_05415"
     CDS             complement(3234..3647)
                     /locus_tag="VFH05_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677683.1"
                     /translation="MTDIVKPSIQAFLVSDQVIEDSVTRKKSLIGLFTHLQAVSFPFQ
                     HQQMGLYFCLTDAEGAYQFDIELIYLNTEQLVCRATLPQIVIGDRLQISDFGINIPSL
                     IFPAPGRYEFRLKMEGHLIAQKDFNVMLLSTHQTA"
     gene            complement(3734..4282)
                     /locus_tag="VFH05_05420"
     CDS             complement(3734..4282)
                     /locus_tag="VFH05_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677684.1"
                     /translation="MMNNSFGTDADMMDLGETSELIVEEEKPEQGLELHVGSNIFRTT
                     NGVVKVQGKEQIVIEVQPDPPALLLTMDFYDERGQRIGHIRRNQLSVVGSARFALCVT
                     TASDATLDDPLTVTVSDRATDRTVIEVSLFHRRKIRFMSAYFHTHKGEAVSITPHYCR
                     IGSGLTMFGDVIEGRGGSAMLG"
     gene            4459..4536
                     /locus_tag="VFH05_05425"
     tRNA            4459..4536
                     /locus_tag="VFH05_05425"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4495..4497,aa:Glu,seq:ctc)
BASE COUNT          985 a         1237 c         1304 g         1058 t
ORIGIN      
        1 cagttccacg ccggtgccgg agatggcgcc aaagccttgc acatcggcat cattggccac
       61 ccgcaccggt tttttcagtc gcgcttccag tacttcagcg atattgtagt cctgccattc
      121 cttagcgaaa ttcggcgctt gcttcacgat gccgttaatg acgactccgg gaaatccgat
      181 tgctacccgg tcgaacgatt cgaattgctt ggacagtgac acgataatgt ccatcacttc
      241 gcccggcatt cccgaggacg gcgagtcccg gcgcagccgg tcgcccactg ccttgccgag
      301 atcattgagc aatgtcgctt tgattccact gcctcctaca tccaccacaa gagtgcggcg
      361 ttgccccccg ccattttcat cggagtcttt gtggtcggtc ttggccttgt tggcccggag
      421 ccgtctttgc ttctttgcca tacccgtcct tgctcccgag acgacttgtc tcagctggga
      481 tgatctgtct tcgttattcg actcgctggg gcctaaacct ttggcccagt gcgtttctag
      541 taacgttccg tcgccggtct aggccgcatg caggacttac gccagcctgt agcatgaggg
      601 gccacatgca atcagaggaa atgttaacgc agggggaacg gcctatcgaa gattaattaa
      661 tggtgagtac cgcggagggg gtcctctggc tcttctccct catccgtagg cagttccatc
      721 atgaggttag ggccgggaag gcggtattgc tcggcagccc aagagcccaa gtcagtcagt
      781 tggcagcgtt ccgaacagaa aggccgccag ggattaccct cccaggcggc tggttgacgg
      841 cagagtgggc aagtcatgcc gacatcctat catagcgatg acgttggggc gcccaggtaa
      901 aaaacctggt cagcttggac ggagaggata gctggttttg gcgtgcagca aacggtgcag
      961 acgattttga tcgagttctg acaggccgag aaattctacg ccgaactccc catgcttgct
     1021 ccatctgaca gcggccgact caatcgtgat gggcgatcct aaatccgttg cacgaatcag
     1081 gattctaact gctgatccgg ggcgcacggc gctcatgctg gtgacggcac acccgccggc
     1141 cgaaagatca aacatcgttc cttcgctgat cgatgtattg ccacttgttg aaaaatagac
     1201 ttgcagctcg accggaacac gagggtactg ccgacattca atcattcggg gactccttgg
     1261 ttatcctagg ttccgaccga ttcgatgcct accggtctct cagaaggaag tgactgatcg
     1321 gtctcccgtc taagcctagc agggatgctt aatctgtaaa gtaaatgcaa gctggcggaa
     1381 gtagagatgt ttcccgtgca gagagagaga agcctaccgg gaggctccgc ttttttcgag
     1441 tttcgcccag gcatccttga gcgagacggt gcggttgaat acaagcctgt cggacaggga
     1501 atcagggtcc atgcaaaaat agccaaggcg ctcgaattga aaccggctgc ctggctgggc
     1561 ctccttgaga ctcggttctg ctcggcagcc gaggagccgc tccaaggact ggggattgag
     1621 gtgggctgtc cagtcttgtt ggtcgacaac accagagagg tcgcctttta aaagggggtt
     1681 gtagaggcgg acctctgctt cgacagcatg agcagccgaa acccaatgga tggtcgcctt
     1741 gacctttctt tgagcactcg gcgcgccgct cttcgtctca acatcatagg tgcagtgtaa
     1801 ttctgtgatc tcccccgtga cggcatcttt catgacgccc acacacttga tgatatacgc
     1861 gtaacgcaaa cggacttccc tgccaggaga aagccgaaaa aactgtttcg gcggatcttc
     1921 caggaaatcg tccctttcga tgtacagggt tcgagagaaa gggattggcc gtgtgccagc
     1981 ggagggatct tcgggattgt tgatggcctc cagttgctcc gtgacgccgt caggatagtt
     2041 gtcgataatg accttcaacg gcttcaggac tgtcatgacg cgaggcgcgc gcttatttag
     2101 atcctctcga atgaagtgct ccagcagctg catttccacg accgaatcgc gttttgacac
     2161 gccaatatgg tcacagaacg cgcggatcgc ttccggcgta tagccgcgcc gccggagccc
     2221 cttgatggtg ggcaagcgcg gatcgtccca gcccgccacg agttttttct cgaccagttc
     2281 aagcaacttg cgtttactca tgacggcctg actgatgttc aaccgggcga actcaatctg
     2341 ttgggggcag gctgcagtgc ccgcttgttc gacgacccaa tcgtagagcg gccggtgatc
     2401 ctcaaactcc aacgtgcaaa tcgaatgggt cacaccttcg aacgcatcag agaggggatg
     2461 agcgtagtca tacgccggat agatgcacca cgtttttcct gtgcgatagt gggcagcgtg
     2521 gcggatacga tagagaatgg gatcccggag attcatattg ggtgaggcca tgttgatttt
     2581 cgcccgcagg acatgtgtcc cgtctgggaa ttcttctgcg cgcatccgac ggaagaggtc
     2641 aaggctttct tcggcgggac ggtcacgata cgggctattt gttcccggtt cggtgagagt
     2701 gcctcggtat cgtctgatgt catcaggtga gaggctatcg acgtaggcca aacctttctg
     2761 gatcaggtcc tcggcgagcc tgtacagccg ctcgaaatag tcagaggcaa aaaacaggtt
     2821 ttctccccag tcgaatccca gccagcgaat atcctcctgt atggcctgga cgtattcggg
     2881 atcttcagta gtgggattgg tatcgtcaaa tcgcaagtgg cacttcccac cgggagggtc
     2941 ttgtgccaac ccaaaattca agcagattga tttggcatgg ccgatgtgca gatagccgtt
     3001 tggctcgggt ggaaagcgcg tcacgacccg gccgccgtgc ttgcctgctg cacggtcggc
     3061 agacacgatc tcgcggatga aattggatgc tgtggttccg gccgtcacgg gttctccgcg
     3121 atcttctcag caatgcagcg acgcgaatca ggaagtgtgt gcggcagttc taccataaga
     3181 gccgtgcagg ggagaagggt gcgggggtcc gtcgagatac ccgaagaaag tgctcaggcg
     3241 gtttggtgtg tggacagcag catgacgtta aaatcttttt gggcgatcaa gtggccttcc
     3301 atcttgaggc ggaattcata ccggcccgga gcgggaaaga tgagcgaggg gatattgatc
     3361 ccaaagtctg aaatctgcag acggtcgcct atgacgattt gcggtaacgt agccctgcat
     3421 acgagttgct cagtgttaag gtagatcaac tcgatgtcga actgatacgc gccttccgca
     3481 tcagtgagac aaaaatacaa acccatctgt tgatgctgga acgggaagct cacggcttgt
     3541 aaatgcgtga agagaccgat caaactcttt ttcctggtca cgctgtcttc gatcacctga
     3601 tcggagacga ggaaggcttg gatgctgggc ttgacgatgt ctgtcatgac cctattctac
     3661 gggagtccgc ccggctgtca aagggataag tctttcaggg tggtcgctgc agcaggaaga
     3721 agggcgccaa caactagcct agcattgcgc ttccgccacg gccttcgatc acgtcgccga
     3781 acatcgttaa tccagagccg atgcggcaat agtgtggagt gatggagacc gcctcgccct
     3841 tgtgagtatg aaaatacgcg ctcataaacc gtatcttccg gcggtggaac aacgacacct
     3901 cgatgaccgt acggtcggtt gctcggtcag acacggtgac tgtcagggga tcgtcgagcg
     3961 tggcatctga ggcggttgtg acgcatagtg cgaagcgggc cgatccgacc accgacaatt
     4021 gattgcgtct gatgtggccg atgcgctgcc cgcgctcgtc gtaaaagtcc atggttaata
     4081 gcagcgccgg cgggtcgggc tggacttcaa ttacgatctg ttcttttcct tgtaccttca
     4141 cgacgccatt cgtcgtgcga aagatgttcg agccgacatg caattccaag ccctgttccg
     4201 gcttctcttc ctccacgatc aattctgagg tctcgccgag atccatcatg tccgcgtcgg
     4261 tcccaaacga attgttcatc atcacatgat cctagtcggc agtatggcga agtggtctct
     4321 cctgacgtgc gaaattaggc caagccacca gcgctgtcaa gatactattt gccaaaaatt
     4381 acccagctgc cgcggtgcgt ttaattgaaa aacgcaagtg cctcatggta ccgtcagcgt
     4441 tccattcctt acacctcggg tcccatcgtc tagcctggcc taggacggag ccctctcaag
     4501 gctcagacac gggttcaaat cccgttggga ccaccacctt gaaattgtta ggtaatttcg
     4561 ctccgtgttt gaggaacatt caaa
//
LOCUS       DASSCQ010000080         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1686000, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000080 DASSCQ010000000
VERSION     DASSCQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686000"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VFH05_05430"
     CDS             <1..1064
                     /locus_tag="VFH05_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6677685.1"
                     /translation="KVLKQGVSLSDVYQTLQSFMGGVMVNFFNRFGRVWQVYVQAEGE
                     FRTQAENVGQFYVRNANGDMVPLSALVTMDIFHGPEFTMRYNEYRSAQLIAGAAPGYS
                     SGQAMKAVEEVFASTMPSGMGLDYMGMSFQEKVAAEGVPATVIFGFSLLFVFLILAAQ
                     YESWALPFSVLLGTPIAVFGAFAALWLLKLVAPQASENDVFTQIGLVMLIGLAAKNAI
                     LIVEFAKVEFEKGKSIAEAALAGARLRLRPILMTAFAFILGVLPLVLATGAGAHGRVL
                     LGLAVFGGMLAASVIAIFLVPVTFYVVESLVHRGTPHSEVPPVPPSGPSQKAGDGELS
                     RQTPRPALSPTHAREHESY"
     gene            1076..2611
                     /locus_tag="VFH05_05435"
     CDS             1076..2611
                     /locus_tag="VFH05_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET6677686.1"
                     /translation="MGTYSWRSASLASVGMLLFMVSACKMGPDYVRPETPKADSWRVP
                     TSTAESIANLAWWDLLKDKELQELIRIALTENQDVRTAVASVEQYRAQLVMTRWDLAP
                     ALSYGGTAFLYQTQGGNATTIPGGGGAIVLPGTTGGDNTTFSNTIGFGSLKWEIDLWG
                     RLRRAVESSEGQLFAQEENQRAVILSLVGSVSEAYFSLRSLDLQVDITKRTLKTWEES
                     VRLSQLKFKQGYIPKLDLDRFEAERAGTAAKLAELEKQVVQTENHLSVLLGRRPASIT
                     RGLKLTEQPIPPEVPAGLPSELLQRRPDLLQAEHTLASATAGIGVAQANRFPQFALGG
                     SLAGANANLNGNQLGPLFIQNISANLAGPLLNATALGYQVTVNETKAQQAALQYEKAI
                     VNALREVEDALISVQKTREQREAQEQQVAALQSAFRLAEQRYQGGRASYLDVLTSQRS
                     LFESELALARTLQGQLVSIVQLYSALGGGWSVTDHPENNLPDPRSANSRQPSAVLVHE
                     PQR"
     gene            2652..3467
                     /locus_tag="VFH05_05440"
     CDS             2652..3467
                     /locus_tag="VFH05_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677687.1"
                     /translation="MTRVPCANRLVILMMACTLLAVSEPVAFGQLSAQQPRASERTPV
                     NPEPSSSALGTQSEQVIVRPTEGYFAGFGGYTFGGKFDAEGTGAFGGVNFGNGSLANS
                     AVVGAKAGGFFPEALNWFGVEAELFNTTPHIEQQGTAAGSHMRVTTLAINAIARAQFA
                     CEKKHLETEQVTERFAFHYVTEFCRLQPYAGVGLGIYWVDISNSAFSAHANFVPGFNA
                     LAGVRYYFTDRIALFTEYKYNRATFNFAGVQGLAGFEGVYSVNHIVGGISYHY"
     gene            3872..>3959
                     /locus_tag="VFH05_05445"
     rRNA            3872..>3959
                     /locus_tag="VFH05_05445"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          841 a         1033 c         1202 g          883 t
ORIGIN      
        1 acaaggtctt gaagcaaggg gtcagtcttt ctgatgttta ccagacgctc cagtctttca
       61 tgggcggcgt catggtgaac ttcttcaacc gattcgggcg ggtctggcag gtctacgtgc
      121 aggcggaagg tgagttccgt acgcaggcag aaaatgtcgg gcaattttac gtacggaacg
      181 cgaacggcga tatggtccca ctctcagcgt tggtgacgat ggacatcttc catggtccgg
      241 agttcaccat gcgatacaac gagtatcgtt cggcgcaact gattgcaggg gcggcgccgg
      301 gatacagttc gggacaagcg atgaaggcgg tagaggaagt gttcgcgagc acgatgccaa
      361 gcggcatggg cctcgattac atgggcatgt ccttccaaga gaaagtggcc gcagagggcg
      421 tccccgcgac ggtgatcttc gggttctcgc ttctcttcgt gtttctgatc ctcgcggcgc
      481 agtacgagag ctgggccctc ccattcagcg tactactcgg cactccgatc gccgtattcg
      541 gggcgttcgc cgccttgtgg ctgctgaagc tggtggcgcc acaggccagc gagaacgacg
      601 tctttaccca gatcggactg gtcatgttga tcggactggc tgcgaagaat gccatcctca
      661 tcgtggagtt cgcgaaagtg gaatttgaaa agggtaagtc gatcgctgag gccgccttgg
      721 cgggggcgcg attgcgactc cgtccgattt tgatgacggc cttcgcgttc attctcgggg
      781 tgcttccgct tgtgctggcc acgggagccg gagcgcatgg tcgtgtgctt cttggcctcg
      841 ccgtgtttgg tggcatgttg gccgcgagcg tcattgcgat cttcctcgtt ccggtcacat
      901 tctacgtcgt cgagagtctc gtgcatcgag ggacgcccca cagcgaggta cccccggtcc
      961 ctccttctgg gccatctcag aaagcaggcg atggagaact atctcggcag acgccccggc
     1021 ctgcgttgtc tccgactcat gcgcgtgaac acgaaagcta ttaaggagcc atgacatggg
     1081 aacatattcc tggcgatccg cctctctcgc aagcgttggg atgcttctgt tcatggtgag
     1141 cgcctgcaag atgggaccgg attatgtgcg cccagaaact ccaaaggctg attcctggcg
     1201 cgtcccgacc tcaacggctg aatccattgc gaatctggcc tggtgggatc ttctcaaaga
     1261 caaagaactg caagaactga ttcgaattgc gctcacagag aatcaggatg tgcggacagc
     1321 ggtcgcttcg gtcgaacagt atcgtgctca gctcgtgatg acgcgatggg acttggcacc
     1381 agcgttgtcc tatgggggca cggcgtttct gtatcaaaca cagggaggca acgcgacgac
     1441 cattcccggc ggaggcggag cgattgtgtt gccggggaca acgggggggg acaatacgac
     1501 cttttccaac accatcggct tcggcagttt gaaatgggag attgatctct ggggtcggct
     1561 acgccgcgcg gtggagtcat cggagggaca actcttcgca caggaagaaa atcaaagggc
     1621 ggtgattctt agtttggtag gaagcgtcag tgaagcctac ttttctctga gatcgctcga
     1681 tttgcaagtg gacattacca agcggacgtt gaaaacctgg gaagaatctg tccggctatc
     1741 tcagctgaaa tttaaacagg gctatattcc gaaattggac ctcgatcggt tcgaagcgga
     1801 gcgtgcgggc actgcggcaa agcttgcgga actcgagaag caagtcgtgc agacggaaaa
     1861 tcacttaagc gtgttgctgg ggcggagacc tgcgtcaatt actcgcggtt tgaaattgac
     1921 ggaacagccc atccccccgg aggtgcctgc gggtttaccg tccgaactct tacaacggcg
     1981 acccgattta cttcaggctg aacacacgct ggcctcggcg acggccggta tcggcgtggc
     2041 gcaagcaaac cgcttcccgc agtttgcatt aggtgggtca ttggctggag ccaatgcgaa
     2101 tctcaacggc aatcagttgg ggcccctgtt cattcagaac atcagcgcga atctggcggg
     2161 accgttgctg aacgccacgg cgttgggcta ccaggtcacg gtcaacgaaa ccaaagcgca
     2221 gcaggctgcg cttcaatatg aaaaggcgat cgtgaatgcc ttgagagaag tggaggatgc
     2281 gctcatctca gtgcaaaaga cacgggagca gcgggaagct caggagcaac aagtggcagc
     2341 actgcaatcg gcgttccgtc tcgctgagca acggtatcaa ggcggacggg caagttacct
     2401 cgacgtgttg accagtcagc gaagcctctt tgaatcggaa ttggcgttgg ctcgtacgct
     2461 gcaaggccaa ctcgtctcaa tcgtccagct ctattcggcg cttggcggtg ggtggtcggt
     2521 gacggaccac ccggaaaaca atcttccgga tccacgaagt gccaactctc gtcagccatc
     2581 agcggttctc gttcatgagc cccaacgctg accgtgcact gtcgatctct gacagagtgc
     2641 cgagtccgat tatgacgaga gtgccttgcg cgaaccgtct cgtcatcctc atgatggcgt
     2701 gcacgttgct cgccgtcagc gaacccgtgg cctttggtca actttctgca caacagcctc
     2761 gagcgtccga acgcacccca gtgaatccag agccatcatc ctcggcgctc ggcacgcaat
     2821 ccgaacaggt catcgtgcgg cccacggaag gctattttgc tggatttggc ggctacacgt
     2881 tcggtgggaa atttgacgcg gagggcacgg gggcgttcgg cggcgtcaat ttcggcaacg
     2941 gcagcttggc taattctgct gtggtcggtg cgaaagctgg aggatttttt cctgaggcgc
     3001 tgaactggtt tggcgtggaa gcggagttgt ttaatacgac gccccacatc gagcagcagg
     3061 gaacggcagc cggcagccat atgcgcgtga cgaccctggc catcaatgcg atcgcccgag
     3121 cgcagtttgc ctgcgagaag aagcatctag aaacagaaca ggtcacggaa cgatttgcct
     3181 ttcactatgt cacggaattt tgtcggctgc agccgtatgc aggggtcggt ttgggtatct
     3241 attgggtgga tatctcaaat agcgccttct cggctcatgc gaactttgtc ccgggattta
     3301 atgccttggc cggcgtcagg tattacttta ccgatcgcat tgccctgttc acagagtaca
     3361 aatataaccg ggcaacgttc aatttcgccg gagtccaagg acttgccggg ttcgagggcg
     3421 tgtacagcgt taatcatatc gtgggaggga tttcttacca ttattaaggc ggccacaact
     3481 ccgctgtcga aagttgactc gtttagaagg attgttatca atcaggagcg ggtttcgttc
     3541 ggagttcggg ccgataatgg tgcgcgtggc gaataggagt ggtcgcgaac cctcttgact
     3601 ccgttcgatc cgattgatat aaggttagtt ccgtttcagt gatcctgcct gttgaccacc
     3661 gtctgacggc gcgtcacggt gcggttctat aagagggaaa ctacccgaag cgttagctcc
     3721 gagcatgtcc cttgacacgg tatgccgtgc gctgtatagt gcgcggctcg cgtgatgaac
     3781 gcgatcgctc tttgacaact gaatagagga cgttgagcaa ggtgtaaggt gtattgaagt
     3841 ggtggccctt ggtccaattt acaagcaaca cctatatgag agtttgatcc tggctcagaa
     3901 cgaacgctgg cggcgcgcct aatacatgca agtcgaacga gaatgtgtag caatacaca
//
LOCUS       DASSCQ010000081        21293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1285263, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000081 DASSCQ010000000
VERSION     DASSCQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 21293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21293
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285263"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFH05_05450"
     CDS             complement(<1..233)
                     /locus_tag="VFH05_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677688.1"
                     /translation="MTTTGMRLWLLSSCFSALMLTGCGGGHDFTYKNITVADGSAKAG
                     DGHSVLFKGSPLSLSGNGIKVGDQLRSVKLAQP"
     gene            complement(274..1203)
                     /locus_tag="VFH05_05455"
     CDS             complement(274..1203)
                     /locus_tag="VFH05_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6677689.1"
                     /translation="MLKRVRFAFELAVVMVMPACSSPLPLPPYFEAFERLPIHTVQVK
                     GETMAYLDSGQGKAVILIHGFGGSMWQWEHQQIPLSSKFRLITPDLVGSGLSAKPEID
                     YRPEQTLEYFVGFMDALKIERATLVGNSMGAGLAIGMALAYPNRVSDLILIDGLPANV
                     REKLTSPSIKRALDSSAPTWLVSLGNWLFGGVVIESVLKEIVHDPSLLTPAVIERSNR
                     NRQRPGLMRPLMTVRDTLPLWEDGFAKRIPDIRHRTLIIWGEEDRVFPLSAGEELQQR
                     INGAVLARIPHAGHIPQWERPDVVNRAMIEFLQ"
     gene            complement(1233..3191)
                     /gene="oadA"
                     /locus_tag="VFH05_05460"
     CDS             complement(1233..3191)
                     /gene="oadA"
                     /locus_tag="VFH05_05460"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HET6677690.1"
                     /translation="MAPKGPSRKKPAAKKRPLGSWATKSSVRPKARPQELAIQPAAGK
                     PILITDVALRDGHQSLLATRMRTEDMLPIAQKLDAVGFWSLEVWGGATFDTCLRFLKE
                     DPWERLRALRAAMPNTKLQMLLRGQNLVGYRHYADDVLERFVERSAANGIDVFRIFDA
                     LNDVRNVDRAVREVKACGKHAEAALSYTVSPVHNIDRYVDLARQLEDLGTDTLCIKDM
                     AGLLAPLDAYQLVRRLKAAVKVPIHLHSHYTSGMASMSALMAILGGLDMLDTAMSPLA
                     GGTSHPATESMVASLQGTPYDTGLALESFLPITEHFRTVRRRYRQFESDFTGVDAEIL
                     TSQIPGGMLSNLAAQLTEQNALDRMKEVLDEVPRVREDMGYPPLVTPTSQIVGTQATL
                     NVLTGERYKVITTETKNYFLGLYGRAPGQVDRNIMARAVGNEEPIKTRPADRLDPELE
                     AVRKDLPASAQSLEDQLSFVLFPAIARDFFEAREKGDLVPEPLEAAAPKGPAVAGELH
                     LAPVEFNLTVHGETYHVKVSGSGRKTDGRKPYYIRVNDKLEEVSLEAIQEVLAGVPEA
                     QDTGKDAKPKRPKPSRPGDVAPPMPGRVVKVLVEKDQRVEAGDPLLIIEAMKMESRVP
                     APLDGKVVAILVSEGENVKTDETVIQLE"
     gene            complement(3226..4671)
                     /gene="accC"
                     /locus_tag="VFH05_05465"
     CDS             complement(3226..4671)
                     /gene="accC"
                     /locus_tag="VFH05_05465"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6677691.1"
                     /translation="MGTSEPEPEMFKKILVANRGEIAMRIIRACRELNIATAAIYSEA
                     DSTGIYVKKADEAYVVGPGPVKGFLDGRLIVDLARRIGADAIHPGYGFLSENAEFAEQ
                     CAAAGITFIGPSPGAITLMGSKVRARELAQSVGVPVVPGTEGGVSEASAALAFARQVG
                     YPVIIKASAGGGGRGLRVVRSDAELRENMEVAAREAQAAFGDGSIFLEKYIERPHHIE
                     FQILADRHGHVIHLGERDCSIQRRHQKLIEIAPSLILTPVLRREMGEAAITIARAVNY
                     DNAGTVEFLLDQDGRFYFIEMNPRLQVEHTVTEQITAIDIVRNQIAIAAGKPLDIRQE
                     EVTLQGHAIQCRINAEDPKNNFMPCTGTVTAYLSPGGIGVRIDGAVYKDYTVPPYYDA
                     LLAKLTVRGRTWEETVSRMRRSLEEYVLRGVKTTIPFMKAIMQDQDFLAGRFDTSYLE
                     THPDLFMYDEFEQPEDLVVAISAAIAAYEGL"
     gene            complement(4839..5309)
                     /locus_tag="VFH05_05470"
     CDS             complement(4839..5309)
                     /locus_tag="VFH05_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET6677692.1"
                     /translation="MQIWRSSVYLMGLVGLCLLSAPESRAEEPSPSDKGAIVEGAGFT
                     LYDMESIKGSDADRVERDPICDRSKRPKIHKVEPDEAKPGQKVTIKGENFGTKECFHG
                     VAFSAAGPAKIEYKFVNESTIEATVPDVKAGMSFIDVVAGGGNARSKGFLVQAK"
     gene            complement(5342..6220)
                     /locus_tag="VFH05_05475"
     CDS             complement(5342..6220)
                     /locus_tag="VFH05_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6677693.1"
                     /translation="MDSSTLSFRFRIPNRNFRAVSAYGSLLLAAGLVLAVAFPAWVRA
                     EVSGNLVIVGNGPELTTIESLSRAFEKANPRAYVDVIWDDHSKPVEMVKAGEAHIAVT
                     GSEDSGLAATQIAWDGIGILVHLSNFTKEVTKQQVADMFSGKVKEWSELGGPETKILL
                     IDRPKNQNIRDAFERQLGIDGKIPDNAKVIAKDDKVIKTVVGTLPPMSAVAYISLTQG
                     LSVVTSGVAVRLLPVDRVEPEVPTVKDGRYPLRRPVLLLSKKDPNPLVEAFGQFALSP
                     AGRQIIAESYIPFERK"
     gene            complement(6230..6559)
                     /locus_tag="VFH05_05480"
     CDS             complement(6230..6559)
                     /locus_tag="VFH05_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6677694.1"
                     /translation="MPLYEYRCGRCEKHFDVTQSVHARVEDTVCPFCQAQEATRLLSS
                     FISTVKGDHKPGFKEMKAYEMHNKRASSFAKLPPLFGARSAPPAIQDVFSGSESQPET
                     PPEKTGS"
     gene            6867..7043
                     /locus_tag="VFH05_05485"
     CDS             6867..7043
                     /locus_tag="VFH05_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677695.1"
                     /translation="MPVLIRRYKGHNGMMQEERIDDDDRIERYFRLFEKEDVKKLETG
                     VKVFIEKDEWQLLP"
     gene            7525..8385
                     /locus_tag="VFH05_05490"
     CDS             7525..8385
                     /locus_tag="VFH05_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6677696.1"
                     /translation="MPRLALLLSTFIWGATFPATKAALEQMPPLTFLCVRFLLGTVLV
                     AAWFALVRHRLRREGGVLSASATATIFLFFGYVLQTVGLYYTTASNSAFLTALYVIIV
                     PLMLRRFDRRVIAATTIATVGLWLLVKPSAVVNLGDVMTVGCAVAFAGHIVCLERFTR
                     LFDAPSLLLWQVSAMTLLFLPMMAWEQPPAHAFTPSTVLIIGLAVTGIFATGAFAVQM
                     WAQQLIPAQQVALIFASEPAYAAWLSWYFLGETLDLQGWIGSGLILIAVLIGAFASSP
                     PEAAAIVEPA"
     gene            8397..8867
                     /locus_tag="VFH05_05495"
     CDS             8397..8867
                     /locus_tag="VFH05_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677697.1"
                     /translation="MAQKFGNGRWVQEGFLDNRIPGTVVGRIVFAAVGPVDLYLRGDF
                     KPDIAGQVIQFRNSRFEDDELAGHVIGDMENPQIGTVNLISFDPHPNMVPHPYVEWFS
                     TRSDHYRFELDPEDAWLVPTNDWEIIDQQSRQIRMALAAAQAIERPEREESDWT"
     gene            complement(8880..9878)
                     /locus_tag="VFH05_05500"
     CDS             complement(8880..9878)
                     /locus_tag="VFH05_05500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6677698.1"
                     /translation="MSDVRTLQPTKAQDLWFTILRWFDSWAGLEHMKTDGVPKMDWLR
                     CIPIIAVHAMCLGVIWVGTSWTAVAVAVAFYFVRMFAITGWYHRYFSHRTFKTSRAVQ
                     FAFAVLGGTCAQRGPLWWAGHHRHHHIASDSADDVHSPRHGGFLWSHIGWFTSQTHFA
                     PRLKGIADFAKYPELRFLDRFDILVPTIAGFAMFGFGKLLEIYAPHLGTNGPQMLIWG
                     FFISTVALIHGTCTINSLSHVYGSQRYETGDDSRNNFLLALITMGEGWHNNHHFYPAS
                     TRQGFYWWEIDMTYYCLKLMEWLGLVWDVRGVPVYVREQKRKEETIGGVPLTAVES"
     gene            10275..11300
                     /gene="hemE"
                     /locus_tag="VFH05_05505"
     CDS             10275..11300
                     /gene="hemE"
                     /locus_tag="VFH05_05505"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET6677699.1"
                     /translation="MNDRFLKACRREPVDCTPVWFMRQAGRYMAEYRSLRAKHSMLSL
                     CKTPELAAQVTLQPIERFPLDAAIIFADILLPLEAMGLHLEFAEGEGPVIHNPVRSRA
                     DVDGLKAIDGTELEYVAEAVRQARKALNGRVPLIGFGGAPFTLASYAIEGGGSRNYVL
                     TKSLMYREPDVWHQLMDKLARVVTGYLRRQIRAGAQAIQLFDSWVGCLSAGDYAEYVK
                     PHVQLIFAGLEREGVPLIHFGTGTTAILRQMREAGGDVIGVDWRSHLDEAWAVIGHDR
                     AVQGNLDPVALYAPLHEIERRVEDILQRAGGRNGHVFNLGHGILPTTPVEHVEAAINI
                     IHKLSMR"
     gene            11376..12317
                     /gene="hemH"
                     /locus_tag="VFH05_05510"
     CDS             11376..12317
                     /gene="hemH"
                     /locus_tag="VFH05_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET6677700.1"
                     /translation="MPKPLAVLLMAMGGPDCLENVEPFLLDVRGGRPTSPELVAEIRE
                     RYRATGGKSPAVGITMNVATKLERRLNESGRAQYRVYVGLRHWHPFIKETYARLIKDE
                     PDHIIGLSMAPQQSSLSTGAYRRKVEEAQAGLSDHRSVSFVGSWNRHPRLIAAIAEHV
                     QEGLRKFSPPTRADVTVLFTAHSLPERIVAMNDPYPDEVKGTVAAVTALLGQQATAFA
                     YQSQGRSGESWLGPTVEATVEELARAGRRHVLVAPIGFLCDHVETLYDIDIELKQSAA
                     RSGVQLERIAMLNDGPALIETLADVVIAHESSLAIPS"
     gene            12314..13762
                     /gene="hemG"
                     /locus_tag="VFH05_05515"
     CDS             12314..13762
                     /gene="hemG"
                     /locus_tag="VFH05_05515"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET6677701.1"
                     /translation="MKNPSTVAVVGGGISGLATAHALMEQAEAKGVALRCTVLESGAA
                     WGGKIVTHTIGDLVTEAGPDSFLSSKPAGLQLVEKLGLNDQLINTNETGKKAFVYLGG
                     RLRELPEGLIAIAPGQIRPFLKSGLLSLGGFARMGMDFVLPAKRSSVDESLASFIRRR
                     FGQQAFERMMEPLMAGIYAGDAEELSVRATFPRFVELEQEHGSVLRGMMSVRARQNGA
                     GRSEAKRTMFVTLKRGLQELVTALVRRLTDQGVLLRSGMTVESLRVRSNQAGRWIYDI
                     VLQDRSSLSADSLVLATPAYVAADLLRPLTPIAAGLLEMISYASTATIALAYPASVIG
                     GAVQGFGFVVPRLEKRDLIAATWTSLKWPHRAPPNQLMVRCYVGGAGRESILALEDSA
                     LIGRVKTELREICGIAVEPTYTEVNRWMHAMPQYRLGHLERLAQAEVALSRYGGVFLT
                     GSAYRGVGIPDCIRDGAVAADKVMRYLSGERL"
     gene            13775..14188
                     /locus_tag="VFH05_05520"
     CDS             13775..14188
                     /locus_tag="VFH05_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677702.1"
                     /translation="MATTNALKILGVAGPFREPREPAFSYDYSIQRPQWPMPHAVRVK
                     VAIAEELDVLRGKILGPVTGSPGQQLLVSQLLTRKIADEKLRIADQDGLLSGRADVVV
                     APFAGPLAHLFSRLESWINEHGEAVRIEIKERVKI"
     gene            complement(14245..14862)
                     /locus_tag="VFH05_05525"
     CDS             complement(14245..14862)
                     /locus_tag="VFH05_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677703.1"
                     /translation="MAQDEGPYQEGKRAGLHPLVVVFGILIGLWLFVALIVPSSRNKQ
                     ATGTEGSAGPVVEDPEAAPVIFKVHATVPDMNAVSLVVPPQATESQVVGLLKRLKKER
                     LAGTLRDLIPATTPGHKLGDHAVAEIYVLSDPTYAEADVIRTLSRGAHAPGGLYPDRV
                     PFEVAMEEIRGHYRIDLNNTGAPDTGSIGFADESGVHSKHFRTLF"
     gene            complement(14862..15710)
                     /gene="mazG"
                     /locus_tag="VFH05_05530"
     CDS             complement(14862..15710)
                     /gene="mazG"
                     /locus_tag="VFH05_05530"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET6677704.1"
                     /translation="MSQGFQKLTELMAALRAPNGCPWDRKQTHESLKPYLLEETYEAL
                     ETIDRGDHKKLAEELGDVLLQVLFHSQIAAESGTFTIDDVLSQLADKLVRRHPHVFPN
                     GAKDPTPTNAEQVLAKWEEIKRAERQAEGRPASAIDGVPQTLPALLRAYQVQARASRV
                     GFDWSHDQAGFDQVLNKIEEEIRELRAALRPPPEEGDTGARQREITAEFGDVLFSLVN
                     LSRFVKVNPEDALRQAVNRFAERFHYIERQAAQAGRGVADLSFNEMNRWWEEAKERES
                     PRDARR"
     gene            15828..16214
                     /locus_tag="VFH05_05535"
     CDS             15828..16214
                     /locus_tag="VFH05_05535"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET6677705.1"
                     /translation="MAGEKEEGFVIRDRRGSGAGSEQPAAPSAAAHEAHAAPTPGTEA
                     GSGVPVTFASFVISLGSSSLMLMGEQLDPQQPAMPVNLPQAKEIIDLLSVLDAKTKGN
                     LSTEEQTVLRDMLYALRMKYVSLTSK"
     gene            16339..18669
                     /locus_tag="VFH05_05540"
     CDS             16339..18669
                     /locus_tag="VFH05_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677706.1"
                     /translation="MTEQPKSMVTPVWTGPERRAAPDQPRGGSTPEGTIFLPEPERHK
                     RHLRPVWILLMFLIPCLALTFYYGQVVVPGGEDPDSFFPTTSYALVLLLINLDLIGFV
                     VLLLLLSRNVIKAYFERRHRLFGSGFRTKLVAAFIGFSLIPTVLLAFVASGLVNKAVD
                     VWFSDQIDHVMRDSYEVAKMQHAGHITLAVNSARAISHEIFREDMLLPEQRDLLVAAM
                     GRKRVEFGVAGIEVYSAKMETLTKSLDAEVPAGVLDLPIGQLVLQAINGKQETNTVQE
                     AATGRLVRAAAPVAASGRGNEVGGVVVVDAYVPESLLAKMDGIGRQYEEYKQIKAMKN
                     PIKAGAYLFVAVITVLILFGASWFGFYVARSITVPIQRLAQATDAIAQGDLSVRIDAK
                     ATDEIGTLIDSFNRMTADLQSSKSTIESANLSLRRTNVELDRRRAYIETVVDTIAAGL
                     LSIDKNGVVTNFNPSGERILGLSAGRVQGRPANEAFKEFGLTLFQTIYDRMLQDQRDD
                     LTLDGTIEVEGKFLTVALHASRMKDEQEQDLGVVLVFEDLTELLKAQKVAAWQEVARR
                     VAHEIKNPLTPIQLSAQRMRKKFMEKAPDFDRVFDESTNVIVNEVASLKHMVDEFSKF
                     ARLPAPQLVLQSLHDVINEVISLYRGGHKDIELIVHLDEDLPHLKFDWEQIKRVLVNL
                     LDNAIQAMNQKGRVWITTQYDTRRRRAVVSIADEGNGIAIEDQEKLFVPYFTRKKTGT
                     GLGLAIVRRIITDHDGHISAGNNQPRGAVFTFELPV"
     gene            18688..20091
                     /locus_tag="VFH05_05545"
     CDS             18688..20091
                     /locus_tag="VFH05_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6677707.1"
                     /translation="MSASILVVDDEEAIVSSLGSILQDEGYEVAVAKSGVEALKIYTV
                     DPPDLMLLDIWMPEMDGLETLRRVKELVPTAQVMVMSGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALEQFRLEEENRSLRTKVQRKFELVGQSPAMQQLRQLIDTAGPT
                     NSRVLVGGENGTGKELVARAIHLQSARADRPFVAVNCAAIPETLIESELFGHEKGSFS
                     GATSMKRGQFEQADGGTLFLDEIGDMSLSTQAKVLRALQEQQFNRVGGTKLLKVDVRV
                     LAASNKDLMKEIEKGTFREDLYYRLNVVPIVVPPLRERREDIPLLIRHFMKLHAEEQG
                     LRTKEVTPDAMAVFQQYEWPGNIRELRNLIERLMIMVPGNVIDGAQATMTLQVKAPAA
                     VAAATPVVQTAASPLFTQPFDSLRDARNAFEKEYIARKLREHHWNISRTAEDLKIERS
                     HLHRKIKLLDVEMRPEA"
     gene            20166..21203
                     /gene="purM"
                     /locus_tag="VFH05_05550"
     CDS             20166..21203
                     /gene="purM"
                     /locus_tag="VFH05_05550"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET6677708.1"
                     /translation="MTTYREAGVDIDAGDEFVDRIKPLVRSTFRPEVLADLGGFGGLF
                     RLQAKQYEDPVLVSGTDGVGTKLKIAFMMDRHDTVGIDLVAMCVNDIVVSGAEPLFFL
                     DYFATGKLAVDKAQQVVAGIAEGCRQAGCALIGGETAEMPSMYAEGEYDLAGFAVGVV
                     DKSKIIDGKSIKPGDAVLGLASSGLHSNGYSLARRVFFDKAKLGLQATLPELNRPLGE
                     VLLTPTRIYAKQILALLREFPIKGIAHITGGGITENLPRVLPDGVCARINRNAWPIPA
                     LFQALSQLGQVDRAEMFRVFNMGIGLILVVPAHLATPVLSKAADLGERGWQIGEIVSW
                     SGSEPVVEYVD"
BASE COUNT         4313 a         6052 c         6404 g         4524 t
ORIGIN      
        1 tcaggttggg cgagtttcac gctccgcagc tggtctccga ctttgatgcc gtttcccgag
       61 agggagagcg gactgccttt gaacagcacg ctatgcccgt cgccggcttt cgcactaccg
      121 tcagcaaccg tgatgttctt atacgtgaag tcgtgtccgc cgccgcagcc ggtcagcatc
      181 agcgcgctga aacaggaaga tagaagccaa aggcgcatgc cagtcgttgt catgaaaatc
      241 ctccgttgtc gtgatcgagt accctcccac cgtttattgc agaaattcta tcatagctcg
      301 atttaccaca tcaggacgtt cccactgagg aatgtgccca gcgtgtggaa tgcgagccag
      361 cacagctccg ttgatgcgtt gctgtaattc ttctccggcc gagaggggaa acacacgatc
      421 ctcttcaccc cagatgatga gggttcggtg gcggatgtca gggatccgtt tggcgaagcc
      481 gtcttcccac agcggaagcg tgtcccggac cgtcatgaga ggtcgcatga ggccgggccg
      541 ctggcggttg cggttggacc gctcaatgac tgcaggagtg agcagtgagg ggtcgtgcac
      601 gatctccttt aacactgatt caatcaccac gccgccaaac agccaattgc ccagtgagac
      661 cagccaggtc ggggcgctgc tgtcgagcgc ccgtttgatc gacgggctcg tgagcttctc
      721 ccgcacattc gccggcaggc catcgatcag tatcaggtcg gacacgcggt tcgggtacgc
      781 cagcgccatg ccgatggcga ggccggcgcc cattgagttg ccgactagcg ttgcccgctc
      841 gatcttgagc gcatccatga agccaacgaa atactccaaa gtttgctcgg gacggtagtc
      901 aatctcaggt ttcgcagaga ggcctgaccc gaccaggtcc ggggtaatga gccggaattt
      961 cgacgagagg ggaatttgct gatgttccca ttgccacatc gatccgccga acccgtggat
     1021 caggatcacc gccttgccct gacccgaatc gagatacgcc atcgtctcgc ctttgacctg
     1081 tacggtatgg atggggagac gctcgaaggc ttcgaaataa ggaggaagcg gaagcggaga
     1141 cgaacaagcc ggcatcacca tgaccacagc gagctcaaag gcgaaacgta ccctcttcag
     1201 cattctgccg gccacgtaat cgccgctgtg cgctactcaa gctgaatgac ggtttcatcg
     1261 gtcttcacat tctcgccctc actgacgaga atggcgacga ctttgccgtc gagcggcgcc
     1321 ggcacacggc tttccatctt catggcctcg atgatcagca gtggatcgcc ggcttcgaca
     1381 cgttgatctt tttccacgag gactttgacg acgcgcccgg gcattggagg ggcgacgtcg
     1441 ccaggtctgg acggcttcgg gcgtttgggt ttggcatctt tgccggtatc ctgcgcctcg
     1501 ggaacgcctg ccaggacctc ttggatggct tcgagcgata cctcttcgag tttgtcgttg
     1561 acgcggatgt agtacggttt gcgtccatcg gtcttgcgcc cggatcccga taccttcaca
     1621 tggtaggttt ctccgtgcac ggtcaggttg aattcaacag gcgccaagtg gagttccccg
     1681 gccacggccg gtccttttgg agccgcggct tcgaggggtt ccggcaccag atctcccttt
     1741 tcccgcgcct cgaaaaagtc acgcgcaatg gcaggaaaga gcacgaagga gagctgatct
     1801 tccaggcttt gggcggaagc aggtagatct ttcctgaccg cttccaactc ggggtccagg
     1861 cggtcggccg gtctcgtttt gatcggctct tcatttccaa ccgcacgggc catgatgttc
     1921 cgatcaacct gccccggcgc acgcccgtaa agcccgagaa aatagttctt ggtctcggtc
     1981 gtgatgactt tgtagcgctc ccccgtcagg acgttgaggg tggcctgggt gccgacgatt
     2041 tggctcgtcg gcgtgaccag cggcggatag cccatgtcct cgcgcacccg cgggacttcg
     2101 tcgagcactt ctttcatgcg gtccaacgcg ttctgttcgg tcaactgcgc cgcgagattg
     2161 gacagcatgc cgccagggat ctgcgaggtc aggatctccg catcgactcc agtaaagtcg
     2221 ctttcaaatt ggcggtagcg gcgacggacc gtccggaagt gctcggtaat gggcaggaac
     2281 gattccagtg ccaggcccgt atcatacggg gtgccttgca gcgatgcgac catggattcc
     2341 gtcgccgggt gcgaggttcc gccggccagc ggcgacatgg ccgtgtccag catgtcgagt
     2401 ccgccgagga tcgccattaa ggcagacatc gacgccatgc ccgaggtgta atgagagtgc
     2461 aggtgaatgg gaactttgac ggcggctttc aggcggcgaa ccagctgata ggcgtcgagc
     2521 ggggccagga ggcctgccat gtccttgatg cagagcgtgt cggtgccgag atcttccaat
     2581 tgcctggcaa gatcgacgta gcgatcgatg ttgtggacgg ggcttaccgt ataactgagc
     2641 gccgcctcgg catgcttgcc gcaggccttc acttcacgga ccgcccggtc cacgttgcgg
     2701 acgtcgttca gcgcgtcaaa gatgcgaaag acgtcaatcc cgttggccgc cgagcgttcg
     2761 acgaatcgct cgagcacgtc atcggcatag tggcgatagc ccacgaggtt ttgtcctcgc
     2821 agaagcatct ggagcttcgt attgggcatg gctgcacgaa gtgcgcgcag ccgttcccaa
     2881 ggatcttctt tgagaaaccg gaggcaggtg tcgaacgtcg cgccgcccca gacctccaac
     2941 gaccaaaagc cgaccgcatc cagtttttgg gcgatcggga gcatatcctc cgtcctcatg
     3001 cgggtagcca ggagcgattg atgcccatcc cgaagggcga cgtcggtgat gaggatgggt
     3061 ttacccgcag ccggttgaat cgcaagttcc tgcggacgag ccttcggccg gacggaggat
     3121 ttcgtggccc aggagccaag cggacgcttc tttgccgccg gctttttcct agaaggtccc
     3181 ttcggtgcca tggtgtcgtc tcagaagaat gagcgccgtc cggtcttaca atccttcata
     3241 agccgcaatt gcggctgaaa ttgccaccac cagatcttcc ggctgctcga attcgtcgta
     3301 catgaagagg tcgggatgcg tctccaggta agaggtgtcg aaacgcccgg cgagaaaatc
     3361 ctggtcctgc atgatggctt tcatgaacgg gatggtcgtc ttcacaccgc gcaagacgta
     3421 ttcttcaagc gatcgccgca tgcggctgac cgtttcttcc caggttcggc cgcggacggt
     3481 cagcttggcc agcaacgcat cgtagtacgg cggtaccgta tagtctttgt agacagcacc
     3541 atcgatccgg acgccgattc cccccggcga taggtaggcg gtcaccgtgc cggtgcaggg
     3601 cataaagttg ttcttgggat cctcggcgtt gatccggcac tgaatggcgt ggccctgcag
     3661 cgtcacctct tcctgccgaa tgtcgagcgg cttgccggct gcaatggcaa tttggttgcg
     3721 cacgatgtcg atcgctgtga tctgttcggt caccgtatgc tcgacctgga ggcggggatt
     3781 catttcgatg aaatagaagc gaccgtcctg atcgagcagg aattcgacgg tcccggcgtt
     3841 atcatagttg acggctcggg cgatcgtgat cgcggcttca cccatttcgc ggcgcagtac
     3901 aggcgtcaga atcaacgacg gggcgatttc aatcagcttc tggtgtcgcc gttggatgga
     3961 acaatcccgt tcgccaagat gaatgacatg tccatgccgg tcggcgagga tttgaaactc
     4021 aatgtgatgg ggccgttcga tgtatttctc gagaaagata ctgccgtcgc cgaaggcagc
     4081 ctgcgcttcg cgtgcggcga cctccatgtt ttctcgcagt tctgcatccg atcgcacgac
     4141 cctgagtcca cgaccgccgc cgccggcact cgctttgatg atgaccggat agccgacttg
     4201 ccgggcaaag gcgagcgccg ccgatgcctc gctcacacct ccttctgtcc cggggacaac
     4261 gggaacgcca accgattggg ccaattcacg agccctgacc ttgctaccca tcagcgtgat
     4321 cgcccccggc gaggggccga tgaatgtaat gccagccgca gcacactgtt ctgcaaactc
     4381 cgcgttttct gacagaaaac cgtagccagg gtgaatggca tcggcgccga tacgccgtgc
     4441 aagatcgacg atgagtcgtc cgtccaggaa ccctttcact ggtccggggc ctacgacgta
     4501 ggcttcgtcg gctttcttga cgtaaatccc ggtcgaatcg gcttccgaat agatggcggc
     4561 agtggcgata ttgagttcac ggcaggcgcg aatgatccgc atcgcgatct cgccgcgatt
     4621 ggccacgagg attttcttga acatctcggg ttcgggctcc gatgttccca tgcgctgctc
     4681 cattcgaccg gaccggcgca aaggaggcgt aagctagcat aagatcgaaa agaatgtcga
     4741 gggagcaagg ggtggctcat cggcacggag ggatggaaac acgcgcaggg cggacttgca
     4801 agggagcgca acctgcgccc cctcgatgtg aacaaattct acttggcttg taccagaaag
     4861 cccttggacc gggcgttacc accaccggcg accacgtcga tgaacgacat gcccgctttc
     4921 acgtcaggca ccgtggcttc gatggtcgac tcgttgacga acttgtactc gatcttggct
     4981 ggtccagcgg cactgaatgc aacgccgtgg aagcattctt tggtcccgaa gttttcgccc
     5041 ttgatcgtca ccttttggcc tggtttggct tcgtccggtt ccactttgtg aatcttcgga
     5101 cgcttcgaac gatcacagat gggatcgcgt tcaacccgat ctgcatctga acccttgatg
     5161 gactccatat cgtagagtgt gaagccggcc ccctccacaa tggccccctt gtcgctcggg
     5221 ctcggctctt cggcccggga ctccggagct gagagcaagc acaggccgac cagccccatg
     5281 aggtagactg aggatcgcca gatttgcata gagacctcct tgaacgataa ggcaggaata
     5341 atcacttccg ctcaaacgga atataggatt cggcgatgat ttgtcgccca gcgggcgata
     5401 atgcgaattg tccaaaggct tcgaccaacg gattcggatc cttctttgac aggagcagga
     5461 cgggacgacg aagcggatag cggccgtctt tgaccgtggg cacttccggt tccactcgat
     5521 caacaggcag taaccggacg gcgacgccgc tggtgaccac agataatccc tgggtcagcg
     5581 aaatatacgc cacggcggac attggaggaa gcgtgccgac gacggtcttg atcactttgt
     5641 cgtccttcgc gatgactttg gcgttgtccg gaattttccc gtcaatgcct agttggcgct
     5701 cgaacgcgtc gcggatgttc tgattcttcg ggcgatcgat caagagaatc ttcgtttccg
     5761 ggccgcccag ctccgaccat tccttgacct tgccggagaa catgtcggcc acctgctgtt
     5821 tcgtgacttc tttcgtgaag ttcgagaggt gaacgagaat gccgatgccg tcccaggcaa
     5881 tttgtgtcgc agcaaggcct gagtcttcgg atccggttac cgcgatatgg gcctcaccag
     5941 ccttgaccat ctcaaccggc ttggaatgat cgtcccagat cacatcgaca tacgcccggg
     6001 ggttcgcttt ctcaaaggcg cgggagaggg attcgatggt ggtgagttcc ggtccatttc
     6061 cgacgatcac cagattacca gacacttcgg cccggaccca ggcagggaat gcgaccgcca
     6121 ggacgagccc tgcggctaag agcagcgatc cataagcaga aacagctcgg aagttgcggt
     6181 tgggaatccg gaagcggaac gaaagggtcg acgagtccat gcggcaaaat cagcttccgg
     6241 tcttttccgg aggagtctcg ggctgcgatt cggaaccaga gaagacgtcc tggattgctg
     6301 gtggcgcgct tcgcgctccg aacaacggcg gcagtttcgc aaaactggac gcccgcttgt
     6361 tgtgcatctc gtatgccttc atttccttga agccgggctt gtgatcgccc ttgacggtcg
     6421 aaatgaagga ggagagcaga cgtgtggcct cttgggcctg gcagaaggga cagaccgtat
     6481 cttccactcg ggcatggacg gattgggtga cgtcgaagtg cttctcacag cggccgcatc
     6541 gatattcgta gagtggcata gcgtttcccc gcccgatttg acgatcaccg ggcggctgcg
     6601 cccttgacca tccgttaaag tttgccttat tgtaacacac ctgcctggtt ccaagactag
     6661 cgggattttc cagggttgga acgtgaaccg ggccgactat catctacagt ggcctcctgc
     6721 agggagttcg aaccccgcac agatacactc gccggtcatt gagggaggca cgtggtggta
     6781 gtgaggcctg gtgacacggc aaaaaagacg ggtggactgt ttcaactttc ctaaagggac
     6841 cgagtcatga agagggagga tgtgcgatgc cagtgctgat tcgacgatac aaaggtcata
     6901 acggcatgat gcaggaagag cggatcgatg acgatgatcg aatcgagcgc tattttcggc
     6961 tcttcgagaa agaggacgtc aagaaactgg aaaccggtgt caaagtgttt atcgaaaagg
     7021 acgaatggca gctgcttccc taatggacgg gcggtcccat cacggtccgc cggttgcgac
     7081 acctgcatga tttattggat acacgtcgcg cactccatcg atcgggtgac gttgcacaaa
     7141 gatcgatgct ctgaagtgcc atcctcgcag acctcaaccg actattgggg gggaggctgg
     7201 tacgattatc cggataagga gcgcgcgctt gcggcgatgg aacaagctgg acccactgac
     7261 cttcgccgct gtcccctgtg caagccttag caaggacgtt ggaaagaggg gcgctgggtt
     7321 tcttctccca gcgcgcagtt cctcaacgcg cttcgctcgc tccacgtgga cgggaatggc
     7381 gtgtcccgcg agaaacaatc gtcgggcggg tgaaagccat cgtcgaagtt ggtggggtgg
     7441 ggaccagagg ctttgtgaac atcctgcaaa tgatctgagc tgacgccgct cttaattccg
     7501 gaaatcccaa cattttccac gttcatgcct cgactcgcac tgctgctcag cacctttatt
     7561 tggggggcca cgtttccggc gaccaaagcg gctctggagc agatgccgcc actgactttc
     7621 ctgtgcgtgc gtttcctgct tggcacggtg ctcgtggcgg cctggtttgc gttggtgcgt
     7681 catcgccttc gccgggaagg cggtgtctta tctgcaagcg cgaccgccac catcttcctg
     7741 tttttcgggt acgtgcttca gaccgttggc ttgtattaca ccaccgcttc gaacagcgca
     7801 tttttgactg ccctgtatgt cattattgtg cctctcatgt tgcgccggtt cgatcgtcgt
     7861 gtgattgccg ccacgaccat tgccacggtg ggattgtggc tgctggtgaa gccaagtgct
     7921 gtcgtgaacc tgggcgacgt catgacagtc gggtgtgccg tcgcctttgc cggccacatc
     7981 gtctgtctgg aacggtttac ccgtcttttt gatgcgccct cgttactctt gtggcaggtg
     8041 agcgccatga cgctgctgtt tttgccgatg atggcctggg agcagccccc ggcccatgcc
     8101 tttacgccct caacggtgct cataatcggt cttgcagtca ccggcatttt cgccacggga
     8161 gcatttgccg tgcaaatgtg ggcgcagcaa ctgattcccg cccagcaagt cgcgttgatt
     8221 tttgcctccg aacccgccta tgccgcctgg ctctcctggt attttctcgg ggaaacactc
     8281 gatctccagg gatggatagg aagcggattg attttgatcg cggtgctgat cggagccttt
     8341 gcatcaagcc cgccagaggc tgccgcaatc gttgaaccag cgtagcggaa ggtctgatgg
     8401 ctcagaaatt cgggaatggg cggtgggtac aggaggggtt tctggataat cgtattccag
     8461 ggaccgtcgt gggccgtatc gtgttcgctg ccgttggtcc agtcgatctg tatctgcgag
     8521 gggatttcaa gcccgacata gccggccagg tcatccagtt tcggaacagc cgcttcgagg
     8581 acgatgagtt agcgggtcac gtgatcggcg atatggagaa tccacagatc ggaaccgtca
     8641 acctcatttc attcgatccc cacccgaata tggttccgca tccttacgtg gaatggtttt
     8701 ccacgcgatc cgatcattat cgatttgaac tggacccgga agacgcatgg ctagtgccga
     8761 ccaatgactg ggaaatcatt gatcagcaaa gccggcagat tcgaatggca ttggccgccg
     8821 cacaggccat cgagcggccc gagcgggaag aatcggattg gacgtaagcg tcggaccgtc
     8881 tagctctcga ctgcggtgag tgggaccccc ccgatcgtct cctcttttcg tttctgctct
     8941 cgaacgtaga caggcactcc gcgcacatcc cacacaaggc ctaaccactc catgagcttc
     9001 aggcagtagt aggtcatgtc gatttcccac cagtagaatc cctgccgcgt cgaagcagga
     9061 tagaaatggt gattgttgtg ccagccttcg cccatcgtga tcagtgccag gaggaagttg
     9121 ttgcggctgt cgtcgcctgt ctcatagcgc tgtgatccgt acacgtgaga gagagagtta
     9181 atcgtgcatg tgccgtggat gagggcaacg gtggaaataa agaaccccca aatgagcatt
     9241 tgcggtccat tcgttccgag atggggggca taaatctcca acagctttcc gaacccaaac
     9301 atcgcaaaac cggcgattgt gggaaccagg atgtcgaatc ggtccagaaa acgcaattcc
     9361 ggatacttgg caaagtcggc gatgcctttt aaccgcgggg caaagtgtgt ctgcgaggta
     9421 aaccatccga tatgagacca cagaaagcct ccatgacgag gcgagtgcac gtcatcggcg
     9481 gagtctgagg cgatgtgatg atggcgatga tggccggccc accagagggg cccacgttgt
     9541 gcgcaggttc cgccgagcac ggcgaatgca aactgcacgg cacgggaagt cttgaacgtg
     9601 cggtgtgaga aataccggtg gtaccaaccc gtgatggcaa acattctcac gaaataaaac
     9661 gcgaccgcga ctgccaccgc cgtccagctg gttcctaccc agatgacgcc caggcacatg
     9721 gcatgaacgg caataatggg aatgcatcgc agccagtcca tcttagggac gccatccgtt
     9781 ttcatgtgct caaggccggc ccaggaatcg aaccatcgca aaatcgtaaa ccaaaggtct
     9841 tgcgccttgg tcggttgaag cgtacgcaca tcggacattc ggattacctc caattaaacc
     9901 cggtgcatct tgccgggcac acacagtgtt ctgaatctca gtaaggcctc ggcacttgaa
     9961 ggtgtttcac gaggcgggct gcacaatcca aggcgctcta gaaactccat caccagccct
    10021 ggacgacggg actgcattat acatcatatt tctccaaata gtagacttgc tcggattctt
    10081 taattgtacc ggatacttcc gcgctgtcta ctcacgcgta aaatcacgaa gttggtgggc
    10141 cggtagtggc gaaaattgac ccagctattg cacatcagtt tcaagtccgt ccgagagaac
    10201 accgagcgtt tgaacgtgaa cccgatcggg cgtcaataga gactttagtc ccatcctgct
    10261 agaatgccgc acgtatgaat gatcgctttc tgaaagcctg cagacgggag ccggtcgatt
    10321 gcacgcccgt ttggttcatg cggcaggccg gtcggtacat ggccgagtac cgctcgcttc
    10381 gcgcgaaaca ctccatgctc tctctctgta aaaccccgga attggcggca caagtcacgc
    10441 ttcaaccgat tgagcgcttt cccctggacg ccgcgattat ctttgccgac attctcctgc
    10501 ccttagaggc gatgggatta cacctggaat ttgccgaggg cgagggaccg gtcatccata
    10561 atccggtacg gtcgcgcgcc gatgtcgacg gcctgaaagc gattgacggc actgaattgg
    10621 agtatgtggc cgaggccgtg agacaggcgc ggaaggcgtt gaacggccgg gtcccgctta
    10681 tcggatttgg cggggcgccg ttcacgctcg ccagctatgc aatcgaaggc ggcgggtccc
    10741 gcaactacgt cctgacaaag tccctgatgt accgtgaacc ggacgtctgg catcagctga
    10801 tggataagct ggccagggtc gtcaccggct atctgaggcg gcagatcagg gcaggagctc
    10861 aagccattca actgttcgat agctgggtcg gctgtctctc ggccggtgat tacgccgagt
    10921 acgtcaaacc gcacgtgcag ctgatttttg ccggattgga gcgcgaaggc gtacccctga
    10981 tccatttcgg gaccggcacg acggcgatct tgcggcaaat gcgtgaagcg ggcggagatg
    11041 tcatcggagt ggattggcgc agtcaccttg atgaggcctg ggccgtgatc gggcatgatc
    11101 gggccgtcca ggggaacctc gatccggttg cgctctacgc acctcttcat gaaattgagc
    11161 gtcgcgtcga ggacattttg cagcgtgcgg ggggccgcaa cggacatgtg ttcaatcttg
    11221 ggcacggtat cctgccgacc acgccggtcg aacatgtcga ggccgcgatc aacatcatcc
    11281 acaaactgag catgagatag cggcgcggcc gttcgggcac attcactgcg tcccgccgcc
    11341 tgctactgaa taacacgaga ggggtgatcg cccgaatgcc gaagccactt gcagtgctgt
    11401 tgatggccat gggaggcccc gactgtctgg agaacgtcga accattcctt ctcgacgtgc
    11461 gtggcggtcg tcccacttcg ccggagctcg tggctgagat acgggagcgc taccgggcga
    11521 ccggcggaaa gtctcccgca gtcggcatca cgatgaacgt ggcgacgaag ctcgaacggc
    11581 ggttgaacga gtccggccgt gcccaatatc gggtttacgt cggcctgcgg cattggcacc
    11641 cgttcatcaa agagacctac gcccggttga tcaaggacga acccgaccat atcatcggat
    11701 tgtctatggc gccgcagcag tcgtcgttaa gcacgggtgc ctaccgccgg aaggtggagg
    11761 aggcccaggc cgggttgagc gatcatcgct cggtgagttt cgtcggaagc tggaaccggc
    11821 acccgcggct gatcgccgcc atcgccgagc acgtccaaga ggggctccgc aagttttctc
    11881 ccccgacacg ggcggacgtg acagtcctct tcacggctca tagcctgccg gaacggatcg
    11941 tcgcaatgaa cgatccctat ccggatgagg tgaagggcac ggtcgcagcg gtgaccgcgc
    12001 tattgggcca acaagccacc gcgtttgcct accaaagcca gggacggtca ggcgagtcct
    12061 ggcttggccc taccgtcgag gcgacggttg aagagttggc tcgagcggga cgacgacatg
    12121 tgctcgtcgc gccgatcggg ttcctgtgcg atcacgtgga gacgttgtac gatatcgata
    12181 tagaattgaa gcaatcggcg gcgcgaagcg gcgttcagct tgagcgcatc gccatgttga
    12241 acgatggtcc cgccttgatc gaaacgctcg cggatgtcgt catcgcccat gaaagttcgt
    12301 tagccatccc ctcgtgaaga atccctcgac agtcgcagtt gtcggtggcg gcatttccgg
    12361 cctggcgacg gcgcatgcgc tcatggagca ggccgaggca aagggagtgg cgctacgttg
    12421 taccgtgctg gaaagcggtg cggcctgggg cggaaagatc gtcacccaca ccatcgggga
    12481 tctcgtcacc gaagccggcc ccgattcttt tctgtccagc aaaccggccg gtttgcagct
    12541 tgtggaaaaa ctcggcttga acgatcagct catcaatacg aatgaaaccg gcaagaaagc
    12601 atttgtctac ctcggcggcc gactccggga actgcccgaa ggcctcatcg ccatcgcgcc
    12661 gggacaaatc cgaccgttcc tgaaaagcgg cctgttgtcc ttgggtggct ttgcccgaat
    12721 gggaatggac ttcgtgttgc ccgccaagcg gtcctcggtt gatgaatcgc tcgcctcgtt
    12781 tatccgccgg cgattcggac aacaggcgtt tgaacgcatg atggaaccgt taatggcagg
    12841 catctacgct ggtgacgccg aagaattgag tgtccgcgcg acgtttccgc gctttgtcga
    12901 gctggagcag gaacatggaa gtgtcctgcg gggcatgatg agcgtgcggg cgaggcagaa
    12961 tggcgccggc cggagtgaag ccaagcggac gatgttcgtc acgctgaagc gtggtctcca
    13021 agagctggtg acggcgttgg tccgccggtt gaccgatcag ggtgtcctgc tgagaagcgg
    13081 catgaccgtg gaatcgttgc gagtgcgttc caatcaggcc gggcggtgga tctacgacat
    13141 agtgctccaa gacaggtcct ctctttcggc ggatagtctc gtgctggcaa ccccggccta
    13201 tgtcgctgcg gatttgttgc ggccactgac gccgatcgcg gcgggactcc ttgagatgat
    13261 ctcttatgcg tcgaccgcga cgatcgcact ggcgtatccg gcctccgtca ttggcggagc
    13321 cgtacaaggg tttgggtttg ttgtcccgcg ccttgagaag cgggacctca tcgccgccac
    13381 ctggacgtcg ctcaagtggc cgcatcgtgc gccaccaaat cagctcatgg tccgttgcta
    13441 cgtgggcggg gcgggacggg aatccattct cgcgctcgag gattcggcgc tcatcggccg
    13501 agtcaaaacg gaactgagag agatctgcgg catcgcagtt gaaccgacct acacggaagt
    13561 aaaccgctgg atgcacgcga tgcctcaata ccgcctcggg caccttgaac gtctcgcgca
    13621 agctgaggtt gcattgagcc gatacggcgg ggtattcctc acgggctccg cctatcgggg
    13681 tgtcgggata cctgattgta tccgcgatgg cgcggttgcg gcagacaaag tcatgcgata
    13741 cctctcggga gaacgtctat aaaggatagg tgcaatggcg acgacgaatg ccctgaagat
    13801 tcttggagtg gccggtcctt ttcgtgaacc gcgcgaaccg gcattttcct acgattattc
    13861 catccagcgg ccccaatggc cgatgcccca tgccgtacgc gtgaaggtcg ccattgcgga
    13921 agagttggac gtgctgcgag gcaagattct tggccccgtc accggctcgc ccgggcaaca
    13981 actgctcgtg tcgcagctcc tcacgcggaa gattgccgac gaaaagttac ggatcgcaga
    14041 tcaggacggc ctgctgtcgg ggcgtgccga tgtcgtcgtg gctccctttg ccggcccgct
    14101 cgcgcactta ttttcccggc ttgaatcctg gatcaacgag catggcgagg ccgtgcgtat
    14161 cgaaatcaag gagcgagtca aaatttagca cgctgccgga agcttcggta tggggagtgg
    14221 cccgcccttg attagaacga ctatctagaa gagtgtccta aaatgcttgg aatgaacccc
    14281 cgactcgtct gcaaacccga tcgatccggt atccggcgcg ccggtattgt tgagatcgat
    14341 gcggtaatgc ccgcggattt cttccatggc cacttcgaag gggacgcgat cgggatacag
    14401 ccccccgggc gcgtgggcgc cccgagagag tgtccgaatc acgtcggcct cggcgtaggt
    14461 tggatcggaa agcacataga tttccgccac ggcatggtct cccagcttgt ggccgggagt
    14521 cgttgcggga atcaggtccc ggagtgtccc ggccaggcgt tctttcttca accgtttcag
    14581 taaccccacg acctgacttt ctgttgcctg cggcggcacg accaggctta cggcattcat
    14641 atcgggaacg gttgcatgga ccttgaaaat cactggagcg gcctcaggat cctcaacgac
    14701 ggggccggca gagccttccg tgccggtggc ctgcttgttc cgcgaactcg ggacaatcag
    14761 ggcgacaaac agccagagcc cgatcaggat gccaaagacg acgaccagcg gatgcaggcc
    14821 tgcacgcttg ccttcctgat aaggaccttc gtcttgtgcc attaccgcct cgcgtcgcgt
    14881 ggcgactccc gctctttcgc ttcttcccac caacggttca tctcgttgaa tgacaggtca
    14941 gcgacaccgc ggcctgcttg ggccgcttgt cgttcgatat agtgaaatcg ttctgcaaaa
    15001 cgattgacgg cctggcgcaa cgcatcttcc ggattcacct tgacgaagcg cgaaagattg
    15061 acgagcgaaa agaggacatc tccgaattct gcagtgatct cccgttgtct ggcgcccgtg
    15121 tcgccttcct ccggcggcgg ccgcaaggcc gcgcgcagtt cgcgaatctc ctcctcgatc
    15181 ttgttcaaca cctgatcgaa ccccgcttgg tcgtgactcc aatcgaaccc cacccgtgac
    15241 gcccgtgcct gtacttgata cgcccgcaag agcgccggca gggtctgcgg gactccgtcg
    15301 atcgccgatg ccgggcggcc ttctgcctga cgctccgcgc gtttgatttc ctcccatttc
    15361 gcgagaacct gttcggcatt ggtcggggta ggatccttgg cgccattcgg aaagacatgg
    15421 ggatgccgcc tcacgagctt gtcagccagt tggctcagca catcgtcgat ggtgaacgtg
    15481 ccggactccg ccgcaatttg gctatgaaag agcacctgaa gcagaacgtc gcccagctct
    15541 tcagcaagct ttttgtgatc gcctcgatcg atggtttcaa gggcctcgta ggtttcttcg
    15601 aggaggtaag gcttcaacga ctcatgcgtc tgtttccggt cccatgggca gccgtttggc
    15661 gcgcgcaacg ctgccatcag ttccgttaat ttctgaaatc cttgggacat tgcccggctc
    15721 cttgtcgttt gccaaaggcg ctattatacc ccgttcctca cggccttcaa cgagttgcgt
    15781 ggctgcaggc cctgtggtag gctcgaaaat ctttcatgga ggcaggcgtg gcaggcgaaa
    15841 aagaggaagg gttcgtcatt cgggatcgtc gaggcagcgg cgctgggtcc gagcaaccgg
    15901 cggcgccctc cgcggcagcg cacgaagccc acgctgcgcc gacaccaggc actgaggccg
    15961 gcagcggcgt gcccgtcacc ttcgcttcct ttgtgatttc gctcgggagc tcgtccctca
    16021 tgctcatggg agagcaattg gatccacagc agcccgccat gccggtcaat ttgcctcagg
    16081 ccaaagagat tattgatctg ctgtcagtct tggatgccaa gacgaagggt aatctctcga
    16141 ccgaagaaca aacggttttg cgggatatgc tctacgcctt acgcatgaag tatgtcagtt
    16201 tgacttcgaa gtaatcctgc ttgtcctgca ctcctgctcc gcagcggagc ctagcaacct
    16261 tttgagcctg gcctctattt ccgctatagt gagtgctctt acccgtcatg cgattgcgcc
    16321 acggagaggg ctcccggggt gactgaacag ccgaaaagca tggtgactcc cgtctggacc
    16381 ggtcctgagc gccgggcggc gccggatcag ccgcgcggcg gatcgacgcc tgagggcacc
    16441 atttttcttc cggaaccgga acggcataag cgtcatttgc ggccggtctg gattctgctg
    16501 atgttcctca ttccttgcct ggcgctgacc ttctattacg gacaggtcgt ggtaccgggc
    16561 ggcgaggatc cggattcctt ttttccgacc accagctatg cgcttgtcct cctgctgatc
    16621 aacctggatc tgatcggctt cgtcgtgctg ttgctgctcc tctctcgaaa cgtcatcaag
    16681 gcctacttcg aacggcgcca ccggttgttc ggctccggct ttcgcacgaa acttgtggcc
    16741 gcgtttatcg gattttcgct catcccgacg gtcctgttgg cttttgtggc cagcggactt
    16801 gtgaataaag cggtggacgt ctggttcagc gatcaaatcg atcacgtcat gcgcgactct
    16861 tatgaagtcg ccaagatgca gcatgccggc cacattacgc ttgccgtaaa cagtgcccgt
    16921 gccatcagcc acgaaatttt cagggaagac atgttgctgc ccgaacagcg cgatctgttg
    16981 gtcgcggcca tggggcgcaa gcgggtggaa tttggtgtgg ccggcatcga agtgtactcc
    17041 gccaagatgg aaacgctgac gaagtcgctc gatgcggagg tgcccgctgg cgtcctcgat
    17101 ctgccaatcg gccagctcgt tctgcaggcg atcaatggaa agcaggaaac gaacaccgtt
    17161 caggaggccg cgacgggcag gctggttcgg gccgcggcgc cggtggccgc aagcggacgg
    17221 ggaaacgaag tcgggggggt ggtcgtggtc gacgcctacg tgcccgaatc gctgttggcc
    17281 aaaatggacg gcatcgggcg gcagtatgaa gagtataagc agatcaaggc gatgaagaac
    17341 cccatcaaag ccggcgccta cttgttcgtc gcggtgatta cggtgttgat tctctttgga
    17401 gcctcctggt tcgggttcta tgtggcgcgc agcatcacgg ttccgatcca gcgattggca
    17461 caagccacgg atgcgatcgc ccaaggcgat ctctcggtgc gcatcgacgc caaagcgacc
    17521 gatgagatcg gcaccttgat tgattcgttc aaccgcatga ctgccgactt acaaagcagc
    17581 aagtcgacga tcgaatccgc caacctgtcc ttacgccgga ccaatgtcga gttggaccgg
    17641 cgccgtgcgt atatcgaaac cgtcgtcgac acgattgccg ccggactgct ctcgatcgac
    17701 aagaacgggg tggtgaccaa tttcaatcca tccggcgaga gaattctcgg cctgtccgca
    17761 ggccgcgtcc agggacgtcc ggcgaacgaa gccttcaagg aattcggtct gacgctgttt
    17821 cagactattt acgatcgcat gcttcaggat cagcgtgatg atctgacgct cgacggtacg
    17881 attgaagtcg aaggaaagtt cctgaccgtc gcgctgcatg cgtccagaat gaaagatgaa
    17941 caggaacagg atctcggcgt agtcctggtg tttgaagact tgaccgaact actcaaggca
    18001 cagaaggttg cggcttggca ggaagtggcg cgccgggtcg cgcatgaaat caagaatcct
    18061 ctcacgccca tccagctatc cgcccagcga atgcggaaaa aattcatgga gaaagccccg
    18121 gacttcgatc gcgtatttga cgagtcgacc aatgtcatcg tgaacgaggt cgccagtctc
    18181 aagcacatgg tcgatgagtt ctcgaagttc gccagattac cggctcctca gttggtgctg
    18241 cagtcgttgc atgacgtgat caacgaagtg atctccttgt atcgcggggg tcataaagac
    18301 atcgaattga tcgtgcatct cgacgaggac ttgccgcact tgaagttcga ttgggaacag
    18361 atcaagcgtg tgctggtcaa tttgctggac aatgccattc aggccatgaa tcaaaaaggg
    18421 cgggtctgga tcacgaccca atatgacacc agacggcgtc gcgccgtggt gtctattgcc
    18481 gatgagggca acggcatcgc gatcgaggat caggagaagc tgtttgtgcc ctatttcacc
    18541 aggaagaaaa caggaaccgg cttggggttg gcgattgtcc gccgcatcat tacggatcat
    18601 gacggccaca tttcggccgg caacaaccaa ccgcgcggcg ctgtgtttac ctttgaattg
    18661 ccggtgtagg ggaggcgctt caagcctatg tcagcgtcga tcttggttgt agatgatgag
    18721 gaagcgatcg tgtcgtcgct gggcagtatt ctccaggacg agggctatga ggttgccgtg
    18781 gccaagagcg gtgtcgaagc gctgaagatc tatacggtcg atcctcccga tctcatgctg
    18841 ctggatattt ggatgccgga aatggacggc ctggagacct tacgccgcgt caaagaactc
    18901 gtgccgaccg ctcaggtcat ggtgatgtcc ggccatggct ccatcgaaac ggcggtcaag
    18961 gcgatcaagc tgggggccta tgactatatc gaaaagccgc tatccctaga aaacgtcacc
    19021 ctgcgggtga agcacgcgtt ggagcaattc cggctggagg aagaaaatcg gtcgctccgc
    19081 accaaggtgc agcggaaatt cgaacttgtc gggcaatctc ccgccatgca gcaattgcgt
    19141 caattgatcg atacggcggg gccgaccaac agccgcgtcc ttgtcggtgg cgagaacggg
    19201 accggcaaag aattggtggc acgtgcgatt catcttcaga gcgctcgagc ggaccgtccg
    19261 ttcgtggcgg tcaattgcgc cgcgatcccc gaaaccctca tcgagagcga gctgttcggg
    19321 cacgaaaagg gatcgtttag cggcgccacg tcgatgaaac gtggacaatt cgagcaggcc
    19381 gacggcggga cgctcttttt ggatgagatc ggcgacatga gtttgagtac ccaggccaag
    19441 gtgcttcgtg cgcttcagga acaacaattc aaccgggtcg gcgggaccaa gctgttgaag
    19501 gtcgatgtcc gcgtgctggc cgcctcaaac aaagatctca tgaaagagat cgaaaaaggc
    19561 acgttccggg aagaccttta ttaccggttg aacgttgtgc cgatcgtcgt gccgccgctt
    19621 cgagaacgcc gggaggatat cccgctgctc atccgccatt tcatgaagct ccacgccgag
    19681 gagcagggct tgcggacgaa agaggtgacg cccgacgcca tggcggtctt tcagcaatat
    19741 gagtggccgg gcaatattcg ggaacttcga aacttgatcg agcgcctgat gatcatggtc
    19801 cccggcaacg tgatcgacgg ggcccaggcc accatgacgc ttcaagtcaa agcacccgca
    19861 gccgtggctg ctgcaactcc tgtagtccag acggcggcca gccctttgtt tacccagccg
    19921 tttgactcgt tgcgcgacgc ccgcaatgcg tttgaaaagg aatatatcgc gcgaaaattg
    19981 cgggaacatc attggaacat ttctcgcacg gccgaggatc tcaagatcga gcgaagccat
    20041 ctgcatcgaa agatcaagct gctcgacgtg gaaatgcggc cggaagcctg agcgcgcggg
    20101 ttctcaccct gctcgtcatc gcgttgacct cttgaagagt gctccgctaa gatgcgtgcc
    20161 ctgctatgac cacctatcgc gaagccggcg tggacatcga cgccggagat gagttcgtcg
    20221 atcgcatcaa gccgctcgtc cgctcaactt tccgcccgga agtgttggca gacctgggag
    20281 gattcggggg actgttccgc ctgcaagcca aacagtatga agatccggtt ctcgtctccg
    20341 gcaccgatgg cgtcggcaca aagctgaaaa tcgcctttat gatggaccgg cacgacacgg
    20401 tgggcatcga tctcgtggcg atgtgcgtta atgacattgt ggtgagtgga gccgagccct
    20461 tgttcttcct ggactatttc gccacgggga agctcgccgt tgacaaggca caacaggttg
    20521 tagccgggat cgccgaaggc tgccgtcagg cgggatgcgc gctgatcggc ggcgaaacgg
    20581 cggagatgcc ctcgatgtat gcagaaggtg agtatgatct tgctggattc gcagtcggag
    20641 tggtcgacaa gtctaagatt atcgatggaa agagcatcaa gcctggcgat gcggtgctcg
    20701 gcttggcgtc ttctggtctg catagcaacg gctactcact ggcacgccgg gtctttttcg
    20761 acaaggccaa gttgggtctt caagccacgc tgccggaact gaaccggccg cttggtgaag
    20821 tcctgctcac gccgacgaga atctacgcga aacagattct cgccctcctg cgggaatttc
    20881 ccattaaagg cattgcccac atcacgggcg ggggcattac cgagaatctg ccccgtgtct
    20941 tgccggatgg cgtgtgcgcc agaatcaaca gaaacgcctg gcccattccc gctctctttc
    21001 aagccttaag ccaattggga caggttgatc gtgcggagat gtttcgcgtg ttcaacatgg
    21061 gcatcgggtt gattctcgtc gtgcctgctc atctggcaac tccggtcctg agcaaagccg
    21121 cagacttggg cgaacgaggc tggcaaatcg gtgagatcgt gtcttggtca ggctcggagc
    21181 ctgtggtcga atatgtcgat taagagagcg actcctcttc ggattgccgt cctcgcctcc
    21241 ggccgcggat ccaaccttca agccatcatc gacggcatcg aagccggaca ggt
//
LOCUS       DASSCQ010000082        70549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_921760, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000082 DASSCQ010000000
VERSION     DASSCQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 70549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70549
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921760"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /gene="uvrA"
                     /locus_tag="VFH05_05555"
     CDS             <1..328
                     /gene="uvrA"
                     /locus_tag="VFH05_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HET6677709.1"
                     /translation="AQRVKLSRELSKRATGRTLYILDEPTTGLHFADVQRLLDVLDRL
                     VEAGNTVLVIEHNLDVIKNADWILDLGPEGGDRGGEIVAEGPPSDIAKAKRSYTGQVL
                     KEAGVR"
     gene            678..1106
                     /locus_tag="VFH05_05560"
     CDS             678..1106
                     /locus_tag="VFH05_05560"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HET6677710.1"
                     /translation="MLPDVARRVLTTCGIIVFGLNLAACNTTKATIDTTVNFFSSTTP
                     NDLFTVDGLVKQEQKINLFAGVAYENLRQEAAAGSGQYVTSLATLYGVPEANHGAFGK
                     VLQQRHAELFATDLVQDNRAHLRMVNVLNRVMNQEPSLMH"
     gene            1275..1661
                     /locus_tag="VFH05_05565"
     CDS             1275..1661
                     /locus_tag="VFH05_05565"
                     /inference="COORDINATES: protein motif:HMM:NF020534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098609 - cell-cell adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin domain-containing protein"
                     /protein_id="HET6677711.1"
                     /translation="MLSLRLGLPMIAAIALSAVPAFAADLETQVIDKNCYIEVFDDDD
                     FDMDDAHYKIQGPKEFASLKDVGGKNWNNDIESVIVGSNAKVKAYSDKDFKGTELAFA
                     PGQRVPNLGKMNMKDDIESMKIACGS"
     gene            1827..4313
                     /locus_tag="VFH05_05570"
     CDS             1827..4313
                     /locus_tag="VFH05_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677712.1"
                     /translation="MPRKKPSPAAATSADGLAKNEDFERLGVFYLGRPYDPVAKQRKA
                     GWILYDSKDLVTHAVCVGMTGSGKTGLCLSLIEEAAIDNVPAILIDPKGDLANLLLTF
                     PGLTAEEFRPWINEDEARKKGLAPAEYAAQQADHWKQGLASWQEDGARIKRFQNSVDA
                     VIYTPGSHAGLPVSILKSFAAPPDDIRNDEELLGERISTTVTGLLGLIGVEADPIQSR
                     EHILISTILNEKWRRKEDLDLAALIHAIQSPPAGKIGVLDVDAFFPSRDRFALSMKLN
                     NLLAAPGFEAWLSGASLDVQELLYTPTGKPRLAIFSIAHLSDAERMFFVTLLLSQVVG
                     WMRGQPGTTSLRALLYMDEIFGYFPPVANPPSKLPLMTLLKQARAFGLGVVLATQNPV
                     DLDYKGLANTGTWFIGRLQTERDKARVLEGLEGASASAGKTFDKNRMEQTLAGLGARI
                     FLLHNVHEDEPVVFETRWALSYLRGPLTRSQIKALMDPRRTESQSSKAASQAGGSVRQ
                     RMLESRPIVDPAVAQYVAPLRGKKPDDSHLVYVPMVLGAGQVRFADSKSGIDATQDVH
                     IMAPVTDGAVALDWGQGVTATISPADLEQSPAEEAQFLAVPPSAGKAKSYDVWKKEFA
                     GWLFRTQQLELLKSAASNEVSRPGESERDFRVRLQQAGRERRDQHIESLRRKYAPKIT
                     ALQERVRRADQQRQKQQAESKTSQVQAAISVGASILGAFLGRKTISATNIGRATTAIR
                     GAGRVLKESRDVNMAEENVAALRQQLADLEAQFGSERDALTAATDPLQEKLESISLKP
                     TKANITVTLVALVWTPHWRSAAGAMTPAWE"
     gene            complement(4327..7080)
                     /locus_tag="VFH05_05575"
     CDS             complement(4327..7080)
                     /locus_tag="VFH05_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6677713.1"
                     /translation="MATEPVNAPVPLLRVLEEEYEALHGALPPSHTALNPHNRLAALY
                     TRIHQLPQKRTALCLSGGGIRSATFALGVIQALAHLSLLTRFDYLSTVSGGGYMGSWL
                     TAWIHRHPKGAGGVMHDLRTVSFDDQDREARPIKWLRQYSNYLSPRLGLLSADSWTLL
                     GSYLRNLTLNWLILVPLIAAVLAVPRLLTALVRGTSGDLGLGPSAPAWSLNLGLLCGL
                     IALTYLHVYRPSLGSLRAHGIWKQFERQQWFLIAGLTPLMIAAFLLTNAWAWYRNAGG
                     HLDDLTFGSWPSRGTFVFGGAFIHAAGWLVSALLLRRWRAFKGRLLVEFLVILWSGAV
                     GGLFLWSVLAETPPGIPVGDFAEWYATFAVPAFLSMFLFTATLFVGVASRYTDDHDRE
                     WWGRAGAWTLIVMLAWTAGGALVIFGPGLLSYTPTLMASVGGVSGVISLVVGFSSKTA
                     ATGKTKPDLLDLVLDRAVRLAAPLFIACLLVLVTLGTSRLLHLIAGLYEPGILDTETI
                     GLGVPSDAWGHALILHHSPLLLLLKLTLSLVIFAGAMSIVININKFSLHSIYRNRLIR
                     AYLGATRLQEYGVERRPNLFTGFDLSDNVQLHELVDSTGRVKKPFHILNMAINLVRGS
                     NLAWQQRKAQSFTASPLFCGSWNLGYRRAKVYGANGRLHRAMTLGTAMAISGAAASPN
                     MGYHSAPAVTFLLAFFNVRLGWWLGNPGPAGRHTFDQSCPRIAVRPLFAETFGLTDAN
                     NPYVYLSDGGHFENLGLYEMVLRRCHCIVVCDASCDSARSFLDLGGAIRKIRIDLGID
                     IDIPTDLLQVQPENRLSRAHYALGIIRYDQVDREASPGLLIYLKPSLTGNEPADVRQY
                     ASCHDEFPHEPTADQFFDESQFESYRALGFHIAEKVFSQPLSKGTGQAEVLFEAFRSG
                     RSS"
     gene            complement(7091..8527)
                     /locus_tag="VFH05_05580"
     CDS             complement(7091..8527)
                     /locus_tag="VFH05_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6677714.1"
                     /translation="MRRPIVAMMSAGLLLAGCFAKIHELPAQPEHAEALFAPLSDQDF
                     MVGLAVSGGGSRAATFAAGALEALAEVPVRFGTENRSVLETVSYMSSVSGGSLATAYY
                     VTNKPPKSEPVLGVNGLSPRYREFFSLYKTAMQSNFQRSALFRQLAFFRALNPTKLAY
                     SLSEVWDEDFLGHTTFAQLYKREQRGDIPRVILNGTVYNSGRRFAFTTLPASDFDYDF
                     IELLTKELKKPDRPVPVTPEGMAIIQKGLERASRQFLPLTFERIGADYRELPLSLAVA
                     TSASFPPVVGPVTYQVAGRPTYLHIGDGGLFDNLGTESLTTLFLKKIPKGSSKSGLII
                     VIDTSFPFDAGGPGLDKSDKGFEVFRDDPSRIVGIMEERANTYQTLLWHSLRTEGVVL
                     PDFAHLRIVVLKHVDAEWTGYDDLPEACRNEFSADVTPAAIKQAVSQIPTLFKIKTPC
                     HGELLLKAARKVVEQSRHRIVNFIEAHQ"
     gene            complement(8765..9319)
                     /locus_tag="VFH05_05585"
     CDS             complement(8765..9319)
                     /locus_tag="VFH05_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HET6677715.1"
                     /translation="MSDQRIGSTISENTNALEHGFEVVVFASRWIQAPLYGGLIVAEL
                     LYAYKFLVELWEMVVHVQGLEETEFMLGVLGLIDVTMVANLLTMVVIGGYATFVSKLS
                     LEGHPDRPDWLSHVDPGTIKVKLAASLIGISSIHLLKAFVDVANENPEHIKWKIFIHM
                     TFLGSAILLAWTDKIMQKDKKHSP"
     gene            complement(9481..11832)
                     /locus_tag="VFH05_05590"
     CDS             complement(9481..11832)
                     /locus_tag="VFH05_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6677716.1"
                     /translation="MTRRAQDRSMTSPPAGKHEPDPGLPDTPTLNAAPAASGVVRNGL
                     TPSHGLVSLEEETRRRLEVESTLQDSEARYRLLYDNNPSMYFTLGADGTVVSVNQFGA
                     TQLGYQPDELIGESVLKVFEPDQHQAVLGQLTVCAASPMKVFQWEIQKVRKDGSTLWV
                     KERAQAVMDHQGQILTLVVCEDITARRLTEDYMRESEERWRALFEHAGVGIAQLGLDG
                     QFLRVNPRLCETLGYSSRTMTRCTFQQFVYPDDLQLILDQIRQLVSGKRPSFAMETRY
                     RRSDDVWVWVEMTVSLVRDGACVPAYFIAVMQNIHDRKQAEAGLARTTQLLQTLVRES
                     PLPIVSLDLAARVTSWNQAATRLFGWSEEEVLGRELPYVPQEEAARAESLWERGLRGE
                     IHGPIELRRCRKDGKILDLLLWPVFVHSEGGDVSTAIGLYVDQSNLKRAEEALLASEE
                     RFSKAFRSSPYPIGITDLETGACLDINDAALEVFGFTREEVIGQTTSVLGIWPDPEAR
                     QEFVERLRTKGTIRNLEVPLKSKRGEVRQFLASTEVIVLNGNECLVTIGTDITEQKRA
                     EEALRRSEVAVRQAFEERERLSQDLHDNLLQSLYAVGMGLELTKQRIQRISRTNAKRL
                     EQSVGQLNNVIREVRSFISHVQPPVVFEQSVADTLRTLAGSFMATGAGEIVVTVDDEA
                     GLRLAPEQRTHVLAIAKEALSNSIRHTKADKRMVTLRRHGNKIRLEVIDNGHGFSPLQ
                     RRKQGMGMGLQNMRARARKLHGRISITSVLNQGTTVTLTIPST"
     gene            complement(11968..13776)
                     /gene="uvrC"
                     /locus_tag="VFH05_05595"
     CDS             complement(11968..13776)
                     /gene="uvrC"
                     /locus_tag="VFH05_05595"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET6677717.1"
                     /translation="MAAANPAVETKLANLPDQPGVYLFKDQTGAVLYIGKAARLADRV
                     RSYFLKGADHTPKTALLVSQIADLETMVTRSELEALILESNLVKRHRPRFNVVLRDDK
                     QYPYLRLPIKDSFPRLSIVRRVQKDGALYYGPYTPAGALRETLKVIKRVFPLATCTID
                     IDGTAERACIEFEIKRCMAPCTGQQTQADYHRIVRQVRQFLEGHDRELVDELRTHMEA
                     AASREEFEEAARLRDRISKIERTLERQRITQTASIDQDVVGLARHGASVDLQLLFVRG
                     GLLIGRKDFFWLESADATDEELVRSSIEQFYNKEGQPPRELLIPVDIPDGDLVARWLS
                     EKRGETVRVLAPERGTKHQLVLLAEENAAAALAEHLRNQELDRQAGDELQRVLRLETA
                     PKRIEGFDVSNTMGNQSVASMVVWEDGRMKKSDYRRFRIASVQGANDFASMQEVVTRR
                     YGGMENLARPDLIVIDGGLGQLAFAKAALKELGQSDLAIIGLAKAKGEKDERVYLPGR
                     KNPIVLRANSPATHLLQRIRDEAHRFAITYHRKLRGKTLVTSHLDQVIGIGSITRTKL
                     LKRFGSLANVAQASEEDLRQAGLSERVVQQLRRELA"
     gene            complement(13818..14687)
                     /gene="dapF"
                     /locus_tag="VFH05_05600"
     CDS             complement(13818..14687)
                     /gene="dapF"
                     /locus_tag="VFH05_05600"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET6677718.1"
                     /translation="MKNGFFRGHGLGNDYLVMDPKELSFKLTPARIAALCDRNWGLGS
                     DGILAPAPSTKADFGLRIFNPDGSEAEKSGNGLRIFARYLHATGKTKKKHFTVETKGG
                     LVTIDLHIDRHGDASAVTVAMGRATFSPSALPCTLPVKELIQQPIDAAGRSLTFTGVS
                     VGNPHCVVFKPAGESWSKQDLLTLGPKLEHHPLFPKRTNVQLAVPTGPKELTILIWER
                     GAGETQASGSSSCAAASAAVRLHLVTSPVTVKMPGGTLTIDVAPDFSLTMKGPVAEVA
                     RGTVSPSFVRSLR"
     gene            complement(14844..15251)
                     /locus_tag="VFH05_05605"
     CDS             complement(14844..15251)
                     /locus_tag="VFH05_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6677719.1"
                     /translation="MTDFKVTKLLHTRMRVSDMDQTIAFYTDVLGLEVLERKTSPRGS
                     HLAFLRVPDSEELIELCSFPPSGAVKVQPDLVHLAFQVDNLDRTMTALQQKGIPITDG
                     PTTTSSGSRFVFIDAPDGYEVELIERPAGANIV"
     gene            complement(15326..15781)
                     /locus_tag="VFH05_05610"
     CDS             complement(15326..15781)
                     /locus_tag="VFH05_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6677720.1"
                     /translation="MRADDCETVITLLGESDPWKTLGYSREDWKRIFCPIPHGRECFV
                     AESEGRIAGIAIVRQKFLLGDYLELLGVAAWARNQGAGKELIRHVESLVFARTKNLFA
                     CVSDFNKGAREFYRKQGYHEIGPMPNFLIPGSAEILLRKTAGPARGQSR"
     gene            complement(15802..16506)
                     /locus_tag="VFH05_05615"
     CDS             complement(15802..16506)
                     /locus_tag="VFH05_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6677721.1"
                     /translation="MRRVHLGLVTVMAVLTLAAATDALGQVITSGPPACPTIALTFDL
                     CPVRKGAGYDKALIDYLIDHHIPATFFMSGRWIAKHDPEVEQLLGIAFFEVGTHGEVH
                     AHLPMHNAEEQRGEILGPVKLLSEHYAHDTVLFRPPFGEYNEVTVDVVKALGLRLVRW
                     NIESGDPDPSLSAEHILVRIEKRAKAGSIVVLHANGKGKHTRQVVERLTADILPRKGL
                     KPTTVSDLLSCKPVNR"
     gene            16607..17758
                     /locus_tag="VFH05_05620"
     CDS             16607..17758
                     /locus_tag="VFH05_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6677722.1"
                     /translation="MTLPSWEIGRALGIPIRVHASWFLVFVFVTWTLATEYLPGALPG
                     LSDSRYWGMGAAAALLLFFSVLLHELGHSYVAQRYRIPIGQITLFLFGGVAQMRAEPP
                     SPKAEFLIAIAGPVVSFALGAFCLGAALAADWLSLPFGGQGFVVLGGLLGVVNVQLGL
                     FNLIPGFPLDGGRALRAGLWAWGRDFHWATSRAALLGLGFGFLLASAGAGLIGGAISG
                     MLHPSTASNGGWLMFIGAFLFGAAWSTRRQASLRLALNATCVRDVMIRAVVTIPSQLS
                     LQAAVDEFFVAYGYGSFPVAEEGRVVGLITVQDVQVISQGLWTWRTVADVMRPASPEL
                     FIQPEWSIMQAMELMVRTGFDRLVVMEDGRPVGLITGSAVARFLQLHKA"
     gene            complement(17783..19138)
                     /locus_tag="VFH05_05625"
     CDS             complement(17783..19138)
                     /locus_tag="VFH05_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677723.1"
                     /translation="MPEDMGKEMLMLGGTLIGFIAALLTVMEKLMDIQHRLTTKKERK
                     PSISHERQTQEPLANIDFFSTKPLRGTSYLLMYETGVIVAAGLMLNYVGLMLSRHLDS
                     ILFLDMTGTALVAFLLGPWWGAIVALLSNSVVNWLLFPESGADVVIFPWSLVSMTGAL
                     YWGWMAREAGFQKYLRTGKSTVLSHAWFLSIFGAGGAVVMSLPGTFVQAALNEHATFA
                     LNPAVAETLHARVLQWEQMVHQYLESVFGLTWGEHVGWWVVNWFQNCVRYVPDKTMSA
                     AVALVVLKYGYPLFERELIHGGPDGERPQDEQLLPLVLGLLYAPSFASLVSSETYGGG
                     AYWPIWTLPWVGILGGYFYLRYWGATASTLSEARLQRAERYARALKPIGKEASYEFCR
                     RLTFMTLIASLLFALCLPIILMDFYRVTFKFFCVVYGFLLVVHLVRIAIAQNISIARA
                     E"
     gene            complement(19223..20110)
                     /locus_tag="VFH05_05630"
     CDS             complement(19223..20110)
                     /locus_tag="VFH05_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6677724.1"
                     /translation="MLMSFWRLLADLTRSFLRHGCASLAASLAFFSLLSLFPLVFMLL
                     YGVSFIVSHEVIGGQVLLSFLKGFLPTLGERLAVELQRISELETVRWLVFLSFAWFGT
                     LVFYELDYALNVVFDSTWKRHPLISTAIAVASQGAVALMLVLSYVATQTVNFLTAYAP
                     HLWGLDLIALAAHDVFLTYTLPFTMAFLTVGALYRYVPRRRPQWREALIGSLTFSLLW
                     VAAKLLFVTYNEYATVYTKLYGSLLEVILLLLWIYYSALLLLLGAVIAHALQQRSQAA
                     AAGSFSPARLVSPSANAGG"
     gene            complement(20205..21692)
                     /locus_tag="VFH05_05635"
     CDS             complement(20205..21692)
                     /locus_tag="VFH05_05635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET6677725.1"
                     /translation="MTSYGSDLIVILPELIVVAAACLVFVLDPLTPHSRKELLAWLSL
                     GSLALCIGLTGGQISVLNLRLPAFSDLVVVDGYARFWKLLLYGVTGLTILMSLPYLKA
                     EGLHLGEYYGFILLALSGMMVMVSGADLLTIYLGTELMSLSLYVMAGLKRASPRSLEA
                     SAKYFVLGAFSSGLLLYGISLLYGLTGSTRLSAIADALGTKGFDDPLLLIATILLTVG
                     FGFKLAVVPFHMWTPDVYQGAPTAVTAFMAVASKAASFAAFLRVFVEGLGGLRADWSN
                     LFVVLCVITLVLGNVVAIVQTNIKRMLAYSSIAHAGYALIGLVAAGRAAGESGGTSGI
                     ASVMLYLAFYAFMTLGAFAVIAMFRKGGLEGEDIDDYAGLAKRQPVAALMMLVFMVSL
                     AGIPPTAGFLGKFYVFMAAVEAGLSWLAAVALIFAAVSAYYYMRVVMLMYMREPEAGV
                     IDSPRLVTSPALSIVLACAVAGVILLGLYPNPVVSLALQSVLMLR"
     gene            complement(21689..23245)
                     /locus_tag="VFH05_05640"
     CDS             complement(21689..23245)
                     /locus_tag="VFH05_05640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET6677726.1"
                     /translation="MHTGGFPWLTVLIFLPLAGAGALYLVKETHARAMALVITVADFL
                     LALPLWWLFDSSSSHMQFVEHVQWITSPPIHYSLGVDGISLPLVLMTALLMPLCVLIS
                     WESITTRVRTFMSVLLVMEGAMIGVFIALDFVLFYVFWEAMLIPMYLLIGVWGGPNRL
                     YAAMKFFLYTLVGSMLLLVAVLVLYFYGGHTFDILALSQVTYPASLQMWLFWAFFAAF
                     AVKVPMFPFHTWLPDAHVEAPTAGSVILASVLLKMGAYGFVRFSLPMLPDASRMMTPL
                     MAALSITAIIYGAYMALAQVDLKKLIAYSSVSHMGFVTLGLFVMNQEGIEGAVMQMVN
                     HGITTGALFLCVGMIYDRTHSRLISDNAGLAKPMPRYATFFVIFTLSSLGLPGTNSFV
                     GEFLVLVGTFLWSKAATAIASLGIILAAVYLLYMVQRIAFGVPSPRELPRLQDIDHRE
                     FGTLVPLVVLVFWIGLFPNPLVSRMHASVTKTLETMARARVAPTSHPSATHRPPVVLT
                     ATGESQAGRP"
     gene            complement(23251..25149)
                     /gene="nuoL"
                     /locus_tag="VFH05_05645"
     CDS             complement(23251..25149)
                     /gene="nuoL"
                     /locus_tag="VFH05_05645"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET6677727.1"
                     /translation="MPYELIPLLPLASFLIVGLLGHWIRDKAHFVAVPAVLGSFLLSA
                     VAFMEVAGGHESSLRLYTWLTSGILDVHIGITIDRLTAVMLLLVTTVSTLVHIYTIGY
                     MHGDPGYARFFSYIALFTFSMLMLVLADNFLQLFVFWEAVGLCSYLLIGHWYERPSAC
                     AAATKAFVVNRVGDFGFLLGLLLVLTTFNSLNYSDVFAQIPSHSGEVINLLDPFGGTL
                     EVSVMTVICLLLFMGAIGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVAR
                     LAPLYDVAPAARDVVAVVGALTMMLGATIALTQTDIKRVVAYSTMSQLGYMMMACGLG
                     AYAAGIYHLLTHGAFKALLFLGCGSVIIALHHEQDMRRMGGLKDKLPVTYWTFVVGSL
                     ALAGFPLTAGFFSKDDLLISAWSAGILGQVLTAFGLVTALLTAFYTFRLVFVTFWGPF
                     RGDRHHGDHVHEPSYSMTVPLIILAVLSIVTGYVGIPDFLQPVFSREGGASLHEGSGA
                     TAIMVLATVMGLLGIAGAYYAYVLHPSLPERWARRWQAAYELSLNKWYADEVYDRLFV
                     RPTFHAAVALWKRMDVATIDAAVNGVARAIAWGGWLLRLVQSGQAQHYALGMALGAVV
                     ILTVLMVM"
     gene            complement(25152..25457)
                     /gene="nuoK"
                     /locus_tag="VFH05_05650"
     CDS             complement(25152..25457)
                     /gene="nuoK"
                     /locus_tag="VFH05_05650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET6677728.1"
                     /translation="MTVPLSYYVALSAVVFLTGLLGVLVRRNVIIILLSVELMLNATN
                     INFVAFSQAFHNIAGQVFVFFALTVAAAEVAVGLAIIIALHRAKSTINVDEFQLLKW"
     gene            complement(25484..25999)
                     /locus_tag="VFH05_05655"
     CDS             complement(25484..25999)
                     /locus_tag="VFH05_05655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET6677729.1"
                     /translation="MDQVFFFYFALVIAVSSVLVVAMRNPIYSALSLLIMFFHVAGLY
                     VTLHAEFLAAVQIIVYAGAILVLYLFVVMLLNLRQDDRYHRQWPVAAAIGSVLAIEAI
                     VLTLLKGGTAAVVRTGQESEAAGLGNTEALGDVLYSTYLFPFEVASLILLVAMIGAII
                     LAKKDLEGRQA"
     gene            complement(26085..26627)
                     /gene="nuoI"
                     /locus_tag="VFH05_05660"
     CDS             complement(26085..26627)
                     /gene="nuoI"
                     /locus_tag="VFH05_05660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET6677730.1"
                     /translation="MKKLVAWFKTIVFYEILVGMKATMSHLLHYKPITVQYPHEKRTL
                     PDNYRGMLGLLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPAEPTKRYAKEYYMDM
                     TRCLFCGMCVDACPVDALGMSREFEWSVYDKRQLHLNKEQLLAIGDRAFPVREKRLEL
                     QHPNVAFFNVSFGHVPQKPN"
     gene            complement(26660..27733)
                     /gene="nuoH"
                     /locus_tag="VFH05_05665"
     CDS             complement(26660..27733)
                     /gene="nuoH"
                     /locus_tag="VFH05_05665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET6677731.1"
                     /translation="MTELSLRLAVSLAQIAAVMGVVMLTVMILTLAERKVLGWMQDRM
                     GPMEVGPYGILQPIADGLKLFFKEDIVPAGANKFMFSLAPILAMVPALIGFAVVPFGP
                     SHKVELFGITIEPFVISDINIGILYILAFTSIGAYGIILGGWASNSKYSLLGGLRSAA
                     QVISYELNVGLAIVGVLILAGSLSLVKITEAQAGGFWHWFVFALPAPQIFAFVVYVIS
                     AVAETNRVPFDLPEAESELVAGFFTEYSGMRFAFFFIAEYANMVLVSCVAAAMFLGGW
                     NAPYPGTILAHVGLEQVAWIENLAWFTVKAYAFLFLFFWLRATLPRLRYDQLMKFGWK
                     VMLPIALGNIVVTSIAVYLFPRG"
     gene            complement(27898..30570)
                     /gene="nuoG"
                     /locus_tag="VFH05_05670"
     CDS             complement(27898..30570)
                     /gene="nuoG"
                     /locus_tag="VFH05_05670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HET6677732.1"
                     /translation="MTASASPTIRLTIDGMEITVPKGTLVIEAARRVGVMIPHFCYHP
                     KLKPDANCRMCLVEIEKMPKLQTSCSTIATDGMSVRTATSVVNDAHKSVLEFILANHP
                     LDCPVCDQGGKCDLQDFSHEYTATGSRFLETKRIFQKEYFSPLIETQMNRCVQCLRCV
                     RYCDEVMDVKALAPSGRGTMTEIKAFGSHPLDCEFCGGCVQICPVGAITSRLSMYEYR
                     PWMLKRADTICQYCGDGCQITVQTKDNELVEVNSSYGAGRNNGDLCARGFFGFHATSR
                     PDRLTKPLVRRNGVLVEATWEEALEYTAEQIARLKRLHGAQAFGGLMSSRCTNEDLYL
                     FQKFMRVVIGTNNLDSSARYGHLNGVQAMRRVQGTHRWTVTFEDIVEADVLLLVGTSI
                     TETNPVTGLRVKEAVKKHMARLLTIELMQPGIDSISNITNLADHHVGVPTAQFGNALI
                     GLLKAVMEGGHVAPSVQKESPDYVTALSQLLERLAWTELEAATGSSPDTFKQMAAAIA
                     QSHRFIVLTGQDLLRSVGGYGACLQLLDLLLLTGKSTEPGCGFAPLAEENNDQGAVEL
                     GAVAELLPDALDLASSDARAKIGRAWNTDIPSTPGATLTDMIERARTGHLKAMFVMGE
                     NPIGTLPSHLGTREACQSLELLICQELFLTETAALAHVVFPAAAPLEKAGTWTNEEGH
                     VQAIRPAVEPAGDSRPDWEILSALSLLLGTPMEYNDSKDLLKEIRSVIPGYGLLGPTP
                     TPQKVDRGAVDRYVSGGYRDDLSTRYTLAPRGPHPQGGDWSMHLTQSLFHSGKLSTRS
                     KGLLEIESRDAIRMNRADGTRLALADGDRVRLSNSRGEMTTSVKLVERVPQGTIWFPD
                     HFAQEATKLFDLTIDAATKVPAFRTTSVTITKIA"
     gene            complement(30574..31881)
                     /gene="nuoF"
                     /locus_tag="VFH05_05675"
     CDS             complement(30574..31881)
                     /gene="nuoF"
                     /locus_tag="VFH05_05675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET6677733.1"
                     /translation="MAQHEPILLKNMSQPGYTGSLADYEQTGGYQAIKKVMGKVSPAE
                     VTQLVIKSGLRGRGGAGFPTGVKWGFLPKDYQGLRYLCCNADESEPGTFKDRQLIERD
                     PHQILEGIVLACYAIAAETAYIYIRGEFVLGARILEKAIGEARRAGYIGKNILGTGVT
                     VDIWVHRGAGAYICGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLAN
                     LPHIINRGSEWFAAIGSPPKSTGTRIFCVSGHVKRPGNYEVPMGITFRELIYEHAGGM
                     RSEKPLKAFIPGGASAPFLTPDHLDVKMDFESVAAAGSMLGSGGVTVMEEGTSMVWAA
                     LRLMEFFYHESCGKCSPCREGSSWLVQTMRRIVNKRGRAQDLETLTDLCKNIAGRTVC
                     AFGDAEVAPIMSTLKHWRPEYIALIHEAEAANLLRPQPVGARG"
     gene            complement(31886..32431)
                     /locus_tag="VFH05_05680"
     CDS             complement(31886..32431)
                     /locus_tag="VFH05_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET6677734.1"
                     /translation="MLRDKYQGEIDEILSRYPVRRSALIPLLYLAQREHGYITEASMT
                     EIASLLKLTPPQVYETATFYTMLNLKQVGKFHIQVCKSLMCALVGSDTVVAWIKSKLG
                     IDPGETTADGVFTLTLVECLAACGTGPMMQVNDDYYERLTEDKFDRIVADLKATGSSA
                     LKSGPFMWPEPLAMKSERPIR"
     gene            complement(32549..34297)
                     /gene="nuoD"
                     /locus_tag="VFH05_05685"
     CDS             complement(32549..34297)
                     /gene="nuoD"
                     /locus_tag="VFH05_05685"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HET6677735.1"
                     /translation="MQPLIETLMTKFPEAVLCVEADTARSEVTVQVAADRILDVARFL
                     HDAPEASFDHLTDICSVDYPEDRQRFEVVYHLHSLTYHRRIRLKARVSEEEPTIASVT
                     GIWKGAEFLEREVYDMMGISFAGHPDLRRILLPEDYAEGFPLRKDFPAEGRGWRSQFE
                     FIPRLDEPPVELAEGEVPESQKEAFRIDSPTGSRRREELLLNMGPQHPSTHGVLRVVL
                     ELDGERIVKATPDLGYLHRGVEKLCEGLAYMQVIPHTDRLDYVCAMANNYAYVRAVEK
                     LLGIAIPERAEYIRTIVAEMQRIVGHLFWLGTQALDIGAMTVFFWTFREREALLDMFE
                     KLCGARLTLNYYRIGGVDSDFTPDLVLRLKSFLDAFPEKVKEYDSLIASNRIWLGRTK
                     HVAVISADDAINFGCSGPVLRGSGVAYDVRKEEPYGVYDKVDWEVPTGKNGDTYDRYW
                     VRMEEMRQSARIIRQCLDQLPQGPIVADAPQYIPPPKQLVMRDMESLIHHFIIFTQGF
                     KPPKAETYCASESPKGELGFFIVSDGSARPYRLKIRAPSFVHMGAFDHMARGYLISDI
                     ITIFGTYDIVMGECDR"
     gene            complement(34301..34780)
                     /locus_tag="VFH05_05690"
     CDS             complement(34301..34780)
                     /locus_tag="VFH05_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET6677736.1"
                     /translation="MSFLERQFEANIITTNLDAVVNWARKSALWPMTFGLACCAIEMI
                     ASVSSRYDIDRFGAGVFRASPRQSDLMIVAGTVTRKMAPVIRRIYDQMPEPRYVISMG
                     SCATSGNHYNSYAVVQGVDQIVPVDVYVPGCPPRPEALMDGLMKLQEKIQREKVFVR"
     gene            complement(35019..35390)
                     /gene="ndhC"
                     /locus_tag="VFH05_05695"
     CDS             complement(35019..35390)
                     /gene="ndhC"
                     /locus_tag="VFH05_05695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET6677737.1"
                     /translation="MADEAVPLNYLPILLFIGITIAFGAIQLLAGRLVRPHRPYRAKL
                     NPYESGSPLFSDARVQFPMRYYIIAMLFVIFDIEVIFIIPWAVRFQSLGLLGLVEMLV
                     FIAILLVGLWYAWKKGALEWD"
     gene            complement(35404..36579)
                     /locus_tag="VFH05_05700"
     CDS             complement(35404..36579)
                     /locus_tag="VFH05_05700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6677738.1"
                     /translation="MSTGHPQLVAAIASEIAREGPIPFVRFMELALYHPQFGYYMRFP
                     EAGVERIGWNGDFYTSSDVHPILGQAIARQAAQIDALLGYPDPFTVVEMGPGKGLLAS
                     HVLSGLQLAPQSLAHRLRYVLIECSPAMRAMQQRHLELWLEQPGCVSWVEDVGSLAQE
                     SVRGLMFSNELADAFPVHRVQKKDHALWEVFVDVRDDGFVERLQGLSTKAIAEYLQRI
                     HVELPEGYRTEVNLRAPGWMSQVANSLEQGVVITIDYGHAAEDLYGPERSRGTLLCYH
                     SQMAGEDPYVRVGEQDITAHVDFTTLATVGEQSGLSVTGFTNQMSFLMGLGIEDMMAQ
                     LAPDSAEFRAAIHLLKPEGMGRTFKILVQHKALDRPALDGLTFRPFFGSALSRHVTA"
     gene            complement(36693..36830)
                     /locus_tag="VFH05_05705"
     CDS             complement(36693..36830)
                     /locus_tag="VFH05_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677739.1"
                     /translation="MSEVSSRNALDETSFGVKQLLRKEAVELAARNERWAAIPPYSTL
                     T"
     gene            complement(37144..39858)
                     /gene="secA"
                     /locus_tag="VFH05_05710"
     CDS             complement(37144..39858)
                     /gene="secA"
                     /locus_tag="VFH05_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET6677740.1"
                     /translation="MLTQILNFIFGSKNDREIKGLMPIVQRINGLESDLIPLSDHALA
                     DKTQTFRKRVAAGETLDDILPEAFAVCREMSRRKLNMRHFDVQLIGGMILHKGRISEM
                     KTGEGKTLVATLPIYLNALEGKGVHLVTVNDYLAKRDAQWMGQLYHALGLSTGVIQHD
                     ASFIYDPAYDAADKRLQHLRPCTRYEAYRADITYGTNNEYGFDYLRDNLVVTALDQCV
                     QRDLNFAIVDEVDSILIDEARTPLIISGPTDQSTDLYYRINAIIPQLKPEHDYTIEEK
                     TKTAALTEEGNVRVEKLLGVDNLYDPDNMDLVHHVVKALQAYALYKRDVDYVVKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYKKLGGMT
                     GTADTEAAEFAKIYNLDVNVVPTNRKMIRLDYADVVYRTEKEKFAAIVEEIKDCHQRG
                     QPVLVGTISIEKSEKIAGMLSRNGIKHNVLNAKQHEREAEIVAQAGRKGAVTIATNMA
                     GRGTDILLGGNADFMFKQVLYREEQLPEERKLAVYEEIRADCEKDKQEVVALGGLHIL
                     GTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFASERVSQLMLKLGMEEG
                     IPIEHGMVTRAIANAQKKVEAHNFEIRKQLLEYDDVMNKQREVIYQHRRAVLAGENLS
                     DDLREMMGTLVESALTVYCPAEQYPEEWDMKGLAEMMQGQFGLDITQGKQDGGESLRD
                     VGRDALLEDLKAQVRDAYDRKEKELGPELMRFLEKNFMLQVIDHHWKDHLLGMDHLRD
                     GIGLRGYGQKDPLIEYKREGYDLFAGMMERIKSDTIERLFLVQAVREGERSAPPPPVQ
                     SRPQPKLTLNRGEEPVSAPTVHRTDDKVGRNDPCPCGSGKKYKKCHGA"
     gene            40025..40336
                     /locus_tag="VFH05_05715"
     CDS             40025..40336
                     /locus_tag="VFH05_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677741.1"
                     /translation="MMSWSPKQSLAVLLVLSLLVIGGLGSAQAISHESHHAHHQKTTH
                     ATVLCAWMCAAGQVLDTVTAPDLIERSPVSRVEFSALPEWSSGFSSLLSSRGPPTDIT
                     I"
     gene            40424..41455
                     /locus_tag="VFH05_05720"
     CDS             40424..41455
                     /locus_tag="VFH05_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET6677742.1"
                     /translation="MTSIGQNRFLLPAWGFSLLLHGLAVVLAMIFAAQIKPVLKEEVF
                     KWDVALVDAVSTESLPESAQPVAPQAQSKPVVTRPSRSTRETALNRVAPHQSVAMVHP
                     ETQTGKPSEQKADLITPPQLERVEPQMAEVRPQTHEPMEQKAVEVPKPDPQPVLEPEK
                     PEPAQKTEPVIAQRQAVAPLPPAEVKEDHVAQPAVSETPAVEPVREASIQNPAQPATS
                     VEPTTASPPKVGLPEAPSQAAQATPGPDTKLDRWVGESLWRRVGELKRYPASARLNGQ
                     EGKVILKAVIRADGHLAEVSVQKSSGHHILDTAAMEAVRLACPLHMKHAISKSEIVVR
                     LPIVYSLAN"
     gene            41477..42784
                     /locus_tag="VFH05_05725"
     CDS             41477..42784
                     /locus_tag="VFH05_05725"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET6677743.1"
                     /translation="MKKGSARTNRLSIQPLHLIVGLIALWPIVPALAETQHPPASFGA
                     KHMSVHHSKTVVGPSVQIDEQGIIFAAWVEEDKDTRTILFARSETPGGPLGTPVPVNR
                     PSESPYYRQESPAVAVRGGEVFVTWSMTHPRTTSDKPFSGELRLSRSIDGGRSFAPST
                     LVNDDEQVIQHAFDAIHIAPDGAVHLAWIDGREGKKEPGTFVARSTDHGRTITKNFKV
                     DDNTCVCCRTAVAASADAVYVAWRKIFDGNVRETVVATSTDGGETFSSPVVVGHDQWA
                     YQACPHRPASLGVDAAGRLYVVWYTEGMDETPAIYLAYSDDRGVTFSEKKQLNRSKGT
                     FPDHPQLAVDPEGRVVVIWEEQSPVRREIVMSHSIDRGQTFSVPFKLNEKNGQTPTVA
                     VNQHGTVVMGWKEHAMPAHRLVVQIMQLPAVAVAESEIISHGP"
     gene            42774..43343
                     /locus_tag="VFH05_05730"
     CDS             42774..43343
                     /locus_tag="VFH05_05730"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6677744.1"
                     /translation="MGRKRYRSFLLALIAGYAVTFGFDASLSGADPSTDPFVALRVSR
                     VPTGNPLETFDLKALDGGTLRSADLIGKVVLLNFWATWCGPCKEEMPSLGRLQEHFDP
                     ARVRVVTVTTDMYPQGIRQFLDQLAIRLPVAFDEDQELSRKLSVRGLPTTLVIGQDGR
                     IVGRAVGPRVWDSSESIAFVRHVLATDTP"
     gene            43340..43645
                     /locus_tag="VFH05_05735"
     CDS             43340..43645
                     /locus_tag="VFH05_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677745.1"
                     /translation="MKRCLSALAIVLVVLYMALAIGAANCLVLHADVPIPHHHSHSHV
                     AHSLLCAWACQASPSVAVAAAVPVVVISVLVAMVRSADSIHRATLFDSESLSRAPPR"
     gene            43759..46095
                     /locus_tag="VFH05_05740"
     CDS             43759..46095
                     /locus_tag="VFH05_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6677746.1"
                     /translation="MWAQRHGSVKRLSIGLIGLALLWSWGADRAMAQATPPADTGGAR
                     ERELRDQLKNILQELEELQQQKDGARPEEERPSIIKEPAESASPEVSARPDIDLADMS
                     IVSQRLQKRPEGISLSATVPAETDSQPTRTMKESLESLPGVVLRQANGPRDFSIMIRG
                     QGAKTTFAVRDIKIYEDGFIQTQSDGLSRLDIHDPWFMRSTEVLRGASSSLYDNYALG
                     GMVHFRTRRGSDINGFETFLSGGSYGFFKQAFAVGQQFENLDVSLFGSNAAEDGFIRN
                     SDYNTQTLNFNLRFTIDDKQSFYFKAITNWLDTRVPTRLTQSQFLTDERQAGGAQTAC
                     TPGVYNPNCANASLLHQQRIDRRTIVGGIYERQLNANTVLTIEADYDVKDINQYFSQI
                     TDNVNPNYKNYVDVRHDGRLAEMPLRSYVGFFVNNMEQEGQTFQNLADGNGTRGLLIQ
                     NNRGTIRNIGGRFREELEFVPKWILAGGLGFEQSMISIQSINYEGATPLRVNADRTYY
                     NWAPEMSLTWKPAEGYRHWIRASTGYGIPQFNNLLRDPFTGQPGTNFDLKPQKNLNTE
                     IGTESRLTKDLVVELVGFWTFFKNEIITQTISGNNVASVNADSSQYRGIEARYDWRPL
                     TGLRFSGAYTHLDARYVNFSDRTAAGFLVRDGNKVPNVPTDIFNTKVEYDHAPTGWGG
                     WIEGSYYNSYFLNNSNTFGIPSYIVMNVNVHKSIEIKNSWIRFAKFYLELDNIADKKY
                     AASGQVIGGETTGAAAGQQAFFAGYGRAVYGGVTLGLF"
     gene            46205..47266
                     /locus_tag="VFH05_05745"
     CDS             46205..47266
                     /locus_tag="VFH05_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6677747.1"
                     /translation="MTVRAARLFWAILSVVSCLFFPGGGSAAAPADSDRRAVEEATEL
                     FRAGEIDAAIVALRKATESGSSSAEAYHLLGQMYFKGKKKPQDAVEAYTHALKLKPAY
                     PDALNDLAEVYVAQGKTAEAEQTLKRAIEVAPKREESYVNLARLYESRHDVPAAINTY
                     QRLLTVRPTSADGLFGLAILHEGQGNNKSARELLARLTKANPAHADGWYQAGRLAERS
                     NDLLEAAYAYRQAIAAKPNLVDAHYNLGFILRSQNKPAEAEKEFLEVIRYRPEYAEAH
                     MNLGVVYTSMNKLEEAEREYERAVALKPDYAEAHYNLGVFYELHRKDMPRALAQYHKY
                     RNLGGRDDRVERIIGTAGR"
     gene            complement(47368..47904)
                     /locus_tag="VFH05_05750"
     CDS             complement(47368..47904)
                     /locus_tag="VFH05_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HET6677748.1"
                     /translation="MRSRWAAVSAMGLCLVVWGCASNPDTGEGSPANQISFLQVKAAP
                     DSYRGQSVLFGGEVLSARRLKDGTRIEILQLPLDRSGEPGTDRMKSQGRFIAMQRDFL
                     DPATIPPGTRVTVTGEVTGSTTLPLDETEYTFPVIETRHIEVLPPPESSLPMRPYMGS
                     HYYGPYGSPYWRPWPYRW"
     gene            47991..48641
                     /locus_tag="VFH05_05755"
     CDS             47991..48641
                     /locus_tag="VFH05_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6677749.1"
                     /translation="MRGKPATWAMMVGMLIAGVLLPTMHTIHASGPPEFKGNYEILQD
                     ERSTHQRGKVKLVEFADFYCPHCHYFDGEGLPILEKEFGKKLETTMVGFPVIRGKLPT
                     PFDMYEQAKLMGKGNEMKKVLFRTIHKDKVTGVLDRSIREVLIKEVGLDPKVFEEGLA
                     SGKPAQLFEEGKKWGERIKLQQTPTILLDGNIKVEKIDPENLKLLIHSILDADSKK"
     gene            48798..51326
                     /locus_tag="VFH05_05760"
     CDS             48798..51326
                     /locus_tag="VFH05_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET6677750.1"
                     /translation="MAVDPVCGMTVDPATAAGQYDYQGVTYYFCATSCLETFKRNPQL
                     ALQAREPEFITLGRKKPLTMMPASTSVESGNIDPVCGMTVQPESAAGSHVHEGKRYYF
                     CSPGCLTRFRENPIHYLIPPEQRLQKPIAVPAGATVEYICPMDPEILETKPGVCRICG
                     MALEPKVVTLDERPNPELVDMSRRLWMSVGPALLVMLFAMADMIPMLRDMVDHSARNW
                     IQLLLATPVVLWGGRPFFERAWMSLVHRAPNMFTLIGLGTGAAYLYSAVVTIAPGILP
                     ASFRAHDGSLAVYFEAAAMITVLVLLGQVLELRARSQTTSAMKALLRLAPKSARRIDG
                     GRETDVPLEHVRVKDLLRVRPGERVPVDGVVIEGTTAVDESMVTGEPLPVAKGPGASV
                     TGGTINGTGSILMRAERVGSETLLARIVQMVSEAQRSRAPIQRVADSVAGYFVPVVVA
                     ISILTAVGWAVWGPEPKLAFALVNAVAVLIIACPCALGLATPMSIMVGTGRGATAGVL
                     VRKAETLEVLGKVDTLVFDKTGTLTEGKPKLVAIRFVPPWNDAEVLRLAAALERRSEH
                     PLASAIIAGADERQLTPGLVEQFQSITGYGVMGVVDQHRVAVGTTQFLREYLKISDPG
                     LAVLEEEAARLRAEGQTTVFVAVDDKAAGLLGVADPMKPSTAEAVRVLKKQGIRLVMV
                     TGDHQLTADAVARRLGISEVHAGVLPDHKGRIIATLKSQGRVVAMAGDGINDAPALAL
                     ADVGIAMGTGTDIAMESAGMTLVKGDLRALVRAVRLSRATMANIRQNLFFAFVYNMVG
                     VPIAAGVLYPLWGLLLNPMIASAAMTFSSVSVIANALRLRHVDL"
     gene            51388..52815
                     /locus_tag="VFH05_05765"
     CDS             51388..52815
                     /locus_tag="VFH05_05765"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET6677751.1"
                     /translation="MRKAGMAASVSFLVRVFAAAVLTLGTSTWGEAAGRLFPVTSSTD
                     LQNQVKATASKVIPAVVSIASTVMVRDQAFSDETLPFGLFREPPARRQYGQGSGVIVS
                     SDGYIITNNHVVADAVDVEVILADRRQFKGKVVATDPKTDVAVVKIQTTNLPTVPWGD
                     SSNLAVGDFVLAIGNPLGLSRTVTFGIVSAVGRADVGVADFEDFIQTDAPINPGNSGG
                     ALVNTNGELVGINTAIASPTGGSVGVGFAIPSNMARAAMQSLIKTGRVVRGFLGASTQ
                     DVTPLLGKIFRLPDVKGAIVTDLQAKGSAEKAGLKRGDVIERFDGRDVMDSGHLRNLI
                     AAAAIGSKHRLDLVREGKMIQVDLTVQEAPRERAKKAQAAAATTSGAHPLSGVVFDDV
                     TPPLARQMDLPVSSGVVVTDIEEGSSAESSGLQPGDVILELNRQPIPNFSTFQRLADP
                     MKSSDLALLLVNRQGTILYIPVRGE"
     gene            52905..53327
                     /gene="nikR"
                     /locus_tag="VFH05_05770"
     CDS             52905..53327
                     /gene="nikR"
                     /locus_tag="VFH05_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HET6677752.1"
                     /translation="MKKLVRFGVSLDHHLLAEFDDVVRRRKYPTRSEALRDLIRDQLV
                     SQEWSGNPGREVVATITFVYDHHVRDLTRKLTHIQHEFQGHVMAGMHVHLDHDHCLEV
                     LVVKGKVSEIKEVADALVSVKGVKHGKLTMTTTGRGLV"
     gene            53331..53624
                     /locus_tag="VFH05_05775"
     CDS             53331..53624
                     /locus_tag="VFH05_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HET6677753.1"
                     /translation="MRLRPRQNQIQRRHGLIHIRHTLGELTSDIAEANPVARATKVLL
                     PINRARLEIAGAEDQTLDRLITRSLTRVTSLVVAPAAGDHMNKNRQATRVCPA"
     gene            53696..55738
                     /locus_tag="VFH05_05780"
     CDS             53696..55738
                     /locus_tag="VFH05_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6677754.1"
                     /translation="MNYRWLVLLSSAVVFNVGPVFAHDPDTVELDVPEVTVLADRSHA
                     SSSQQFIPDKEYILQPQGRPAQVLRLIPGFVAIEHSGGAGKADQYFLRGFDADHGTDV
                     AFFSDGMPINFRSHAHGQGYTDLNFIIPETIEGLDVSKGAYLPEYGDFDTAGAVNFRT
                     RDVVKEGIVQAAGGQFDTQRYILMFSPTKDRIRTLFAGEGYYTNGPFLNDNRYFRANL
                     LGKVTTNLSSRDELSLTATFHKAQWNASGEIPLRTVTDGTLDRFGAIDPSEGGKTLRS
                     TARLNYHYDTQSGGELFANAYGQYYKYDLYTNFTFFLNDPVNGDGFQQHDTRVMYGGD
                     LGYKHRAQFYGMPSTGTIGFQTRVDDIHARLGTQVRRNPLSTTADSNILEASYAPYLK
                     AEVQPLPWMRLTGGLRAETFTFNVQNRCPDCAEQPAGRTSSSIVLPKANLILGPWFQT
                     EFFANYGEGYHSNDARSAVTPASSPLARARTYELGARSKPWGPEGMELIATLWAIDLR
                     SELVFVGDEGTTEIRGPTRRRGVEVAARGQIWGPIYINGSLTWSKSEFVNGDAIPLAP
                     ELTAYGAVLVQWPEGLRSQIQATYLGVRPLIEDRSANAPSWIDIDLSERYILPVKLPY
                     GRLEAFLFIQNLLNTKWEQATFYFASRLRNEAAGVNDVHFVPGNPRFFMGGLAWYF"
     gene            55895..56431
                     /locus_tag="VFH05_05785"
     CDS             55895..56431
                     /locus_tag="VFH05_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6677755.1"
                     /translation="MKRRHRILSKSYFNRSTLVVAKSLLGKYLVRQNGKETIAGKIVE
                     VEAYVGPHDLACHASKGRTLRTDVLFGPPGISYVYLIYGMYHCLNVVTERAEFPAAVL
                     IRALEIDGALVDGPGRLCRALDIDLSLNRLDMTAGRHLWIEDRGLRVPQAQVGTFPRI
                     GVAYAGSWAEKPYRFRII"
     gene            complement(56500..56868)
                     /locus_tag="VFH05_05790"
     CDS             complement(56500..56868)
                     /locus_tag="VFH05_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677756.1"
                     /translation="MKLLFGTVAMVSGVLFAVSMASANPALLPKHEGYPMKNSGSPVN
                     GQATANDPGQSDARGASTLTKAAGFDDNHVSQNLKKVDNDRITASEGAGRLPKVQGPQ
                     IQIAPPVNSATKITGDRKID"
     gene            complement(57237..59057)
                     /locus_tag="VFH05_05795"
     CDS             complement(57237..59057)
                     /locus_tag="VFH05_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677757.1"
                     /translation="MTPAIAQSKPPSETQLQRALNSVLNDIPVDSALAAILHRENGPL
                     VGHAARGFTARDVQAILRTLSAPALITAGAPDQDGGRTMRLRMITPGAKSLLGVPLRY
                     RNRTYGFLVIGRKESAAFTKKERGLMDQASEDVTKALEREKLFDMNVVLSRPLVNQEQ
                     VSVAASSPPLLLSAPVSQLTPQLQENITALLTDAAETVSYDRSWVCHYDPIAGNVEVL
                     GMAGDLKGEHKDGKKDMRPGHRLALDSSAAGWAIRHRKPRVDHDLASTQGRFLDHKQL
                     FKDRFQSSLVFPFFVRGQVGGTVTLASREADRYQPTDARTLEPLILKLADLLQAPPPA
                     AAAPAPVVDEGGTSAAPAQPPAISQEPIIRKQERQAAIGEFSAFLATEVREPLGSIRA
                     QLEEVTGEGVLDFDPQTRVENAMRDLMRVEAIVNEILDFAKPLELNRRLVRIPEVIES
                     ALTVVANDLEITRIQVEKEYAPVLAPVRGDEAKLQQVFLSIFKNACEAMSPGGRLTIQ
                     VAQHRAGRGIEDHILIKNDGAPIPPEIVDKVFEPFFTTKKAGSGLGLATVKKIVEEHA
                     GSIAIGSAPGEGTTVTIRLPGVSRGPSFRHRGRSRRPHRR"
     gene            complement(59221..59679)
                     /locus_tag="VFH05_05800"
     CDS             complement(59221..59679)
                     /locus_tag="VFH05_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6677758.1"
                     /translation="MRIVHLSQQQAAPFSGGGFHSSMVRLFITGIAVFLAVATVPGLE
                     ADSFGAGVAAVLVLTLLNLLLRPLLFILTLPLIVLSLGLFLVVLNALLLELTAYMVKG
                     FTVAGFWPAVGGALVISVVTSVLNLFTGDYRQVDTRRTQPREPKVINPPE"
     gene            complement(59691..60053)
                     /locus_tag="VFH05_05805"
     CDS             complement(59691..60053)
                     /locus_tag="VFH05_05805"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HET6677759.1"
                     /translation="MFHSFVLAVLCMMLPTAALAHGTGQHVLGTVVAIDSKHLEVKTQ
                     KGTTVEVLINKQTRFKEKGNPKGANLPSVGDRVVVEAIKDDKALTATEVHFSSLKKTA
                     QEPAPLPQQTPAAGTPAP"
     gene            complement(60086..60703)
                     /locus_tag="VFH05_05810"
     CDS             complement(60086..60703)
                     /locus_tag="VFH05_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6677760.1"
                     /translation="MKAKLFGAAVLIVIAGLGILSWLGYQLFTTGFSAKTEPHALEVW
                     MARQIRHLAIPIDKRNLANPVPLTAEVLREGRAHFADHCAVCHANDGSGQTPIGKNVY
                     PQAPDLRLRETQSMSDGELFWVIHNGIRFTAMPAWGTGDPEKDLDSWKLVHFTRHLPQ
                     LTQEELDEMKTLNPKTPNELLEEAAFDRFLEGTDTGGKSVEGHHH"
     gene            complement(60805..61839)
                     /gene="hpnA"
                     /locus_tag="VFH05_05815"
     CDS             complement(60805..61839)
                     /gene="hpnA"
                     /locus_tag="VFH05_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HET6677761.1"
                     /translation="MRTLCHGGPAIRHLLFHMKALVTGATGFVGGAVARALIRAGVNV
                     RVLIRSNSDFKNLSGLSVERVEGDLRDPVSLRSALTGCHHLYHVAAHYALWAKDPAIF
                     YDINVTGTRNMLSAARQAGVERTVYCSTIGAIGLPPGGGLGSEETPVSLDQMAGHYKR
                     SKYLAEQEVLKFAHEGLPVVIVNPSAPVGAGDVRPTPTGQVIVDFMKGRMPAYIETGM
                     NIVDVEDVAAGHLLAMEKGRQGERYILGCKNLMLREVFDILSGLTGVKAPSLRLPRWT
                     VLPLAYVNEWMANVTGQPPRIPLEGVKMAKYRMHYDCGKAIRELGIPQTPPEAALEKA
                     VRWFRSHGYA"
     gene            complement(61877..62767)
                     /locus_tag="VFH05_05820"
     CDS             complement(61877..62767)
                     /locus_tag="VFH05_05820"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET6677762.1"
                     /translation="MNIKDYLEQKRVEVDRFLDEVTPPAAMPPATLHDSMRYSLLGGG
                     KRIRPILTIASAEALGTNPPGLLAAACSLELIHTYSLIHDDLPAMDNDDFRRGKPTNH
                     KVYGEAMAILAGDALLTMAFDLISRPDLMKGCDPVRQARIIQELAFGSGNMGMVGGQV
                     LDILAEHQDIDLLTLQNIHKHKTGMLIRAAVRMGAIAGGANDRQLDDLTGYAEDIGLA
                     FQIADDVLNVTGTREELGKNPNTDAERGKKTYPTFYGVEGAKQLADACVSRAIDRLSS
                     FGSTADPLREIARYITSRRS"
     gene            63184..63600
                     /locus_tag="VFH05_05825"
     CDS             63184..63600
                     /locus_tag="VFH05_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677763.1"
                     /translation="MQNLPRGPVTPLDIRWRNSRISRRCWLVTPERSSRPHPAHPAWH
                     HLFAQVVRYGLAPAILSISGFFTANFLASGLYTWPRTSRILALCLTLLVLSYEFVYKE
                     QCAWTGSKAVARAALLYSCAIPYLAGMVLMFALWNW"
     gene            63611..63985
                     /locus_tag="VFH05_05830"
     CDS             63611..63985
                     /locus_tag="VFH05_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677764.1"
                     /translation="MGQVFHSGAFLQQCWSVHPLCISVRRITAGNQLILACTSCRFAH
                     HLRLGDVAVRTSASEQTAPSTGESDAQLPSNIRFAACLDAHAAALTLREMDVFEDRAV
                     IRCAECRRLYVLTVMAFETHQK"
     gene            64150..64767
                     /locus_tag="VFH05_05835"
     CDS             64150..64767
                     /locus_tag="VFH05_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677765.1"
                     /translation="MTNVPSFTDEETALLIDAQFFQKKATISEKIRAQLEKTGEALKA
                     ELSRGQLPALPHDHLSAQFVRGEHLENFPYQYLDCPKYFHGPEKRTFRTLVWWGHHVA
                     CAWLLEGQLTKQYKARLVDRFHTLAGRHLELSLAPTLWEWKRGEGYTLPITHDRKAQT
                     AAVLAERAFLKIVRFIPFDDARLRSGDLAGLSLEAFRAMLPIVSL"
     gene            64921..65568
                     /locus_tag="VFH05_05840"
     CDS             64921..65568
                     /locus_tag="VFH05_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677766.1"
                     /translation="MMHSLMRVGAGILLSVGLMASSGCGGSGETLHLEVLPPQQPVQV
                     AEPEAVKIVIEPFEDRRADKHRIGMRTHLWGGVTNFNVTGEKPGEVYAQALADRLRAS
                     GWLDRSWDVRVAPAGSAPTADIVISGQIFEFAANAKSRMFSTYITTSNKLTITARNNV
                     DRSSTSRSLEGAQTDTVVWFSEDDVKKLMTATVKDTLDRYLADTTISQRAVRPSR"
     gene            65591..66652
                     /locus_tag="VFH05_05845"
     CDS             65591..66652
                     /locus_tag="VFH05_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677767.1"
                     /translation="MRERRWETRGPMTFTQVLAVGERLAALGLKPAVAAKDVICYVEE
                     WSVEAPEDFDSLDAWSTEDVTLVHVRDGWRGDFFLLAGAYHTTYLHYQNVGTYCSISH
                     PWRIRERMKLHENRSMLWLGFRHAHSFLRVRLHTREVVTPGETRDDARREQWLDERRT
                     AFLEAITTVGLPIETAIEKGQLVLRPNDPTVPFFCSWPDAFGPCQFEYNTSDAYEFLV
                     PASQLAATYAPEPAGVRAYLTGFSDEALAEFARIEPVAHLAYRCSVHCPLDDLPEIRS
                     AIEPDGRLYATLCEFQTQELIPDGDEASAIIGVVGSRTGFGIEARLNKAPLKEDLMAG
                     WLERLIGEPLVYAPLPAFV"
     gene            66760..67869
                     /locus_tag="VFH05_05850"
     CDS             66760..67869
                     /locus_tag="VFH05_05850"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HET6677768.1"
                     /translation="MRPLSRVQSLLAKPFMPAVFFLSGVTYDTLTLTRIDRLLDNLVL
                     LLYLLLLGLLIVLTGRLGIEPPPDPAQLKTLSPVGRWVLQTRPFYPMASQFLLGGLFS
                     AYAIFYFRSATLTSTAVFFSVLVIFLVANEFLRDRLSNLRLLVSLYALVCFGFFTFFL
                     PVMTGYMNVLVFLGGAVLSGAVTFRVVELIYRNNPDRSRREAVGVSVPAFGVIALLVG
                     FYFLNWIPPVPLSMTFGGIYHEVKKTDDRFELSFERSWYQVWKQSNSTFPSDEPVYCF
                     TAVFAPVSLNATVYHHWYFRPSRELPFRHADRIPVRISGGREGGYRAYTFKQRLDPGD
                     WRVDVETEDGRIIGRVSVRVEMRAEPQPPLTTIVY"
     gene            67978..68346
                     /locus_tag="VFH05_05855"
     CDS             67978..68346
                     /locus_tag="VFH05_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HET6677769.1"
                     /translation="MPPRPLPPCPSSPNCVSTLASHADTQHAIAPFRYQKSLAEAKEL
                     LKALIASLPRTRLIEEDGEYLHYEFTSLLIRFVDDVEFVFDEGSKTIHFRSASRVGYG
                     DLGVNRRRMENIRSSLSGRI"
     gene            complement(68366..70378)
                     /locus_tag="VFH05_05860"
     CDS             complement(68366..70378)
                     /locus_tag="VFH05_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6677770.1"
                     /translation="MHLPTYDETGAQAAGCPLRRRRVTIDPIAYLAFCLLLWASCEPI
                     ATKTYQVRQRAFTYTPSSLIQDYQEEYAYIEQRRRAVRSSDRDKEGDTFPRSNTVPSH
                     LSGLAFSGGGIRSATFHLGILQALQDMGSLPQIDYLSTVSGGSYIAGWMLAHLGQELD
                     DSYGNLVQTPDQATLLDPHGDFVTHLRHHAGFIREGGFWEGPKLIWGYLWRLIPYYVW
                     DLVLHIKTPPDIGNELHLFTPYQHRIEVTYLRGKHETPLVRLNRKDVDAPYLIINGNL
                     VNRGRPRAYETESDQPYRDNYNFEFTRDYTGSDGLGYVQSAGFGLPVVEVQTQDGSPA
                     SFNPRQVVVQDLTEGTCHKTWGERIRPHACVRLSQAMTASGAGLDLDSLVQEWYRDDI
                     ARLTLGILTAPFNLNNEYQTWNFARRHNTTLGTIWDYVLMLSIQRIWPDTKSRWIEIT
                     DGSFYDNLGAQTLLRRQVAHLIVGDATLDTTWQYDYLYNLQARIKGYFGPGVEWCGEI
                     PPKNEIVWYRRFWVIRPDGVPTVLHYLKPYAYNPILFRDNPSLLAPGKIYVSHEPESG
                     RSLVTHPLTSPPVNPTHLDANWLLPLVTDRASSEKVKLALKKVSKFVDSPIGEEFPQT
                     STIFQWYKLEEFEAYRQLGSLMGWAYLSTVTFSADELAPAVSCRAL"
     gene            complement(70324..>70549)
                     /locus_tag="VFH05_05865"
     CDS             complement(70324..>70549)
                     /locus_tag="VFH05_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677771.1"
                     /translation="RLTRFLVLGDNAAMSTSPNAPASPASEIPDRLCTWCKVPMNKRL
                     VASGPFLHYTCPKCIFQHTTRRGPKPPAAH"
BASE COUNT        15069 a        20068 c        20737 g        14675 t
ORIGIN      
        1 ggctcagcga gtaaaattat ctcgcgagtt gtccaagcgc gcgaccggac ggacgctgta
       61 cattctcgac gaacccacaa ccggcctgca cttcgccgat gtgcagcggt tgctcgatgt
      121 cttggatcgg ctcgtcgaag ctggcaacac ggtcctcgtg atcgagcaca atcttgacgt
      181 gatcaagaac gccgattgga tcctcgatct cggccctgaa ggaggggacc gcggcggtga
      241 aattgtggcc gaggggcccc cctcagacat tgccaaggcc aagcgttcgt acacgggcca
      301 ggtcctcaaa gaggcaggag tacgatgaac ctcctgctgg gactgaggat gagtcccgcc
      361 actagcgttt tgtcatcttc atccagacgc acactcgaga gttccatcac ggcatcgtgt
      421 cagtcatcca acctgtcccg gagcagggac aacttgtcca cacggacggc cgaacgggcg
      481 gcgatttgat gatcttcctg cggagacgcc ctcgtttgcc gtgacatcca ggcggcctta
      541 gccagcttgc gagttcggcc cgtcccttgc ttactctagg acctgggagt ttcgccagca
      601 cttacgccta ggggcgaatt gagcggtccg gtagggtccg ctttttctac cgattcattt
      661 acccgaaagg aggagcgatg ttacctgatg tggcacgtcg agttttgacc acttgtggaa
      721 tcatcgtgtt cggattgaac cttgcggcat gcaacacgac caaggcgacg atcgacacga
      781 cagtcaactt tttctcgagc actacgccaa acgacctgtt tacggtggat ggcctggtga
      841 agcaggagca gaagatcaac ctgttcgccg gggtggcgta tgaaaacctg cgccaggaag
      901 ccgcagcagg aagcgggcag tatgtgacgt cgctggccac cttgtacggc gtccctgagg
      961 ccaaccatgg tgcctttggg aaggtcttgc agcagcggca cgcggaatta ttcgcaactg
     1021 acttggtcca agacaaccgc gcccatctgc gtatggtcaa cgtgttgaac cgggtcatga
     1081 atcaagaacc ctcgttaatg cactaaaaga cccgaatagg aaatcagacg gaggaataga
     1141 tgggtgcgat ggggaaaggc gttattttgt ggctgctcgg agtgccagtg agcctactgg
     1201 tcgtgctgtg gtttcttggt atctttcgat aaccacgcac cgagtgagat tactcacaca
     1261 taaaggagtg cactatgtta tcgctgagac ttgggctgcc gatgatcgcc gcaattgcgt
     1321 tgagtgccgt accggccttc gccgcagatc ttgaaacaca ggtaatagac aagaactgct
     1381 acatcgaagt cttcgatgat gacgatttcg acatggacga cgcccattac aaaatacaag
     1441 gtccgaaaga atttgcgtca ttgaaagacg tgggcgggaa gaattggaac aacgacattg
     1501 aaagcgtgat cgtcggttcc aacgcgaagg tcaaagctta ctcggacaag gatttcaaag
     1561 ggacagaact cgcatttgcc ccgggccaga gggtgccgaa tctcggtaaa atgaatatga
     1621 aggacgacat cgaatcaatg aagattgcgt gcggtagctg agttctcaac gccgggagac
     1681 ggccgcatgg ccgcctcccg gctccctctc ccacctgacc aaccactgaa acagggcact
     1741 gcatccttgc gggcaacctt gaagcggttg gccgtccgac ctacaatgct tcgtcactgc
     1801 tctgtctttc ctgctcatga ggatccatgc cacggaaaaa gccttcaccc gcggcggcga
     1861 catcggctga cgggttagcg aagaacgagg attttgagcg gctgggcgtc ttctatcttg
     1921 gtcggccgta tgaccccgtc gcgaagcaaa ggaaagccgg gtggattctg tacgactcga
     1981 aggacctggt gacccatgcg gtttgcgtgg gcatgacggg gagcggcaag acggggttgt
     2041 gtttgtccct catcgaagag gccgcgatag acaatgtacc ggcgattctg atcgatccaa
     2101 agggcgacct cgccaattta cttttgacct ttcccgggct taccgctgaa gaattccggc
     2161 cgtggattaa cgaagacgag gcgcgaaaaa aggggctcgc gccggctgaa tatgcggcgc
     2221 agcaggcgga tcattggaaa cagggcctcg cgtcctggca ggaggatggg gcccgcatca
     2281 agcggtttca gaattccgtc gacgccgtga tttacacgcc ggggagtcat gcaggcttac
     2341 ccgtctcgat tctcaaatcc tttgcagccc ctcccgacga tatccgcaac gacgaggaat
     2401 tgttggggga gcggatcagt acgaccgtga ccggcctgct gggattgatc ggcgtggaag
     2461 cggatccgat tcaaagccga gagcatattc tgatttcgac gattctgaat gaaaaatgga
     2521 ggcggaaaga agatctcgac cttgcggccc tcattcacgc catccaatcc ccccccgccg
     2581 ggaagatcgg cgttttggac gtcgatgcct tctttccttc cagggaccga tttgcgctct
     2641 caatgaaact gaacaacctg ctggctgcgc ccgggttcga agcctggctg agcggagcgt
     2701 cgttggacgt ccaggaactg ctctacacgc ccacaggcaa gccccgcctg gctattttct
     2761 cgatcgcaca tctgagcgac gctgaacgga tgttttttgt caccttgttg ctgagccagg
     2821 tagtcggctg gatgcgcggc cagcccggga ccaccagctt gcgcgcgctg ctctatatgg
     2881 acgagatctt cggctacttt ccgccggtgg caaatcctcc gtcgaaactg ccgctgatga
     2941 cgttgctcaa gcaggccagg gcattcgggc tcggcgtagt attggcgaca cagaatccgg
     3001 tggatctcga ttacaagggc ttggccaaca ctggcacgtg gttcatcggc cggctccaga
     3061 ctgaacgtga taaagcacgt gtgctcgaag gtttagaagg cgcctctgcc agcgcaggca
     3121 agacattcga caaaaatcgt atggaacaaa cgctggcagg actgggcgct cgcatcttct
     3181 tgttgcataa cgtccatgaa gatgagccgg tcgtgttcga aacgcgctgg gctctttcct
     3241 acctacgagg cccgctcacg cgctcgcaga tcaaggcatt aatggatccg cgcagaaccg
     3301 agtcgcaatc atcgaaggcc gcctctcaag cgggaggcag cgttcggcaa cgcatgctcg
     3361 agtcgcggcc gatagtggat cccgctgtcg cgcagtatgt tgcgcccctg cgtggcaaaa
     3421 agccggacga cagtcacctg gtctatgtgc ccatggtgct gggcgccggc caggtgcgtt
     3481 ttgctgacag caagagcggc atcgatgcga cgcaggacgt ccatattatg gctcccgtga
     3541 ccgatggtgc ggtcgccctc gactggggtc agggcgtcac agcaacaata tcaccggcgg
     3601 atctcgagca aagtcccgcc gaagaagcgc aattccttgc ggttccgccc agcgcgggca
     3661 aagccaagag ctatgacgtc tggaagaaag aatttgcagg ctggctcttc cggacgcagc
     3721 aattggagct gttgaagagt gcggcttcga acgaagtgtc gaggccggga gaatccgaac
     3781 gcgattttcg tgttcgttta caacaggccg gtcgcgagcg gcgtgaccag cacattgaat
     3841 cgctgcggcg aaaatatgcg cccaagatta ccgctcttca ggagcgtgtt cgccgtgcag
     3901 accagcagag acaaaaacag caggccgagt cgaaaaccag tcaagtccaa gccgccattt
     3961 cggtgggcgc gtcgatcctg ggggcattcc tgggccgaaa aaccatcagt gcgacgaaca
     4021 ttggccgggc cacgacggcc attcgcggcg ccggtcgtgt gctgaaggag tcccgcgacg
     4081 tgaatatggc cgaagagaac gtggccgcgc tgcggcagca actggcagac ctcgaagcgc
     4141 aattcggatc agagcgtgac gcactcactg cggcgaccga tccacttcag gagaaactgg
     4201 aatccatttc tctcaagccg accaaggcga acatcaccgt cacgctcgtg gcgttagtct
     4261 ggacgccgca ctggcgatcc gccgccggcg ccatgacgcc cgcctgggaa tagtccgaag
     4321 aaaagctcag ctgcttcggc ccgagcggaa tgcctcgaac agcacttcgg cttgtccggt
     4381 cccctttgac agcggctgac tgaagacttt ctcggcgatg tgaaatccga gtgccctata
     4441 ggactcaaac tgcgattcgt caaaaaactg atccgcggtc ggttcgtgcg ggaactcgtc
     4501 atgacagctc gcatattgcc gcacgtccgc cggttcattc cccgtcagcg acggtttcaa
     4561 atagatcagc aggccgggag aagcctctcg atcgacttga tcgtagcgga tgatgcccag
     4621 tgcatagtgg gctcggctca gccggttttc cggctggacc tgaagaaggt cggtgggaat
     4681 gtcgatgtct attccaagat cgatgcgaat tttccgaatg gcacccccga gatccaaaaa
     4741 ggaccgggcg ctgtcacaac tcgcatcaca gaccacgatg cagtggcacc ggcgaaggac
     4801 catttcgtac agtcccagat tttcgaaatg gccgccgtcg gacaggtaga catagggatt
     4861 attcgcatcc gtcaaaccga acgtttcggc gaacaaggga cgcaccgcaa tgcgtgggca
     4921 ggactggtca aacgtgtgac ggccggccgg tcccggattg ccaagccacc accccagcct
     4981 gacgttaaag aacgcgagca aaaacgtcac agcaggagcc gagtggtaac ccatgttggg
     5041 actagccgca gctccggaga tagccatggc ggttcccagg gtcatggctc tgtggagccg
     5101 gccattcgct ccatacacct tcgcccgtcg gtatccgaga ttccaacttc cgcaaaagag
     5161 cggggaagcc gtgaatgatt gcgctttccg ttgttgccag gccaaattgc ttcctcggac
     5221 gagattaatc gccatattca ggatatgaaa cggcttcttg actcgcccgg tgctgtctac
     5281 gagttcatgc agctgcacgt tgtcgctgag gtcgaatccg gtgaagaggt tgggacggcg
     5341 ttcgacacca tactcctgaa ggcgcgtggc accgagatag gctcggatca gccgattgcg
     5401 gtaaatcgag tggagagaaa atttattgat attgatgacg atggacatgg cccctgcgaa
     5461 gatgacaagg gacaaggtaa gcttcaacag caggagcaga gggctgtggt gaagaatgag
     5521 cgcatgtccc cacgcatcgg atggcactcc taagccaatg gtctccgtgt cgaggatgcc
     5581 aggctcatac aagcccgcaa tcaggtgaag aagccggctc gtgcccagcg tgaccaagac
     5641 aagcagacaa gcaatgaaga gcggagcggc cagccgcacc gcccgatcga gtaccaaatc
     5701 cagcaggtcg ggcttcgtct tccccgtcgc cgctgtcttg gagctgaatc caaccaccag
     5761 ggagatcaca ccggacacgc ctcccacgct ggccatgagc gtcggtgtgt aggacagaag
     5821 tccaggcccg aatatgacca gcgcgccccc cgcggtccac gccaacatga caatgagtgt
     5881 ccaggctccc gcccgtcccc accattcccg gtcgtgatcg tctgtatatc ggctggcaac
     5941 gcccacgaac agagttgcgg tgaacaggaa catggagaga aaggccggca ccgcaaacgt
     6001 ggcataccat tctgcaaaat cgcccaccgg aatcccggga ggagtttccg cgagcaccga
     6061 ccacaggaag agaccaccca cggcgccaga ccacaggatg accagaaact cgaccagaag
     6121 acgccccttg aaggcacgcc accggcgcaa gagcaaagcc gagaccagcc atccggcggc
     6181 atgtatgaag gcgccgccaa acacgaatgt accgcgactc ggccagctgc cgaacgtcag
     6241 atcatcgaga tgaccgccgg cgtttcgata ccaagcccag gcgttggtca gaagaaacgc
     6301 ggcgatcatg agcggcgtca ggccggcaat aagaaaccat tgttgccgct caaactgctt
     6361 ccagataccg tgagcgcgaa gcgatccgag actggggcga tacacatgga gataggtcag
     6421 ggcaatcaat ccgcacagca ggcccagatt gaggctccag gccggtgctg acggaccaag
     6481 accgaggtct ccgctggtcc cacgaaccaa cgctgtcaga agacgaggga cagccaagac
     6541 ggcggcgatg agaggcacca ggatcaacca attgagcgtg agattacgca gataactgcc
     6601 gaggagagtc cacgaatcgg cggataacaa tccgaggcgg gggctcaggt agttgctata
     6661 ttgacgcaac cacttgatag gcctcgcttc acggtcctgg tcatcgaatg atacggtccg
     6721 caagtcatgc atcacgccgc ctgcgccctt tggatgtcga tgaatccatg cggttaacca
     6781 actccccatg taaccgcctc ctgaaacggt ggagaggtag tcgaaccggg tgaggagtga
     6841 gaggtgagca agcgcttgaa tgactcccaa tgcgaacgtg gcacttcgga tccctcctcc
     6901 cgataaacag agggcggtcc gtttctgtgg cagttggtgt atgcgagtgt aaagggcggc
     6961 gagccggttg tgaggattga gtgcagtatg tgacggaggc aaggccccat gaagcgcttc
     7021 gtattcctcc tcgaggacgc ggaggagggg aacgggagca ttgactggct cagtggccat
     7081 gatggctcgc ctattggtgt gcttcgatga aattgacgat acgatgccgc gactgttcca
     7141 ccactttgcg cgccgctttc aacagcaact ccccgtgaca aggcgtcttg atcttgaaca
     7201 gggtcgggat ctggctgacg gcttgcttga ttgcagccgg tgtcacgtcg gcggaaaact
     7261 cattccggca tgcttccggc agatcatcgt atcccgtcca ctccgcatcg acgtgtttta
     7321 agacgacaat tctcagatgg gcgaaatctg gaagcacgac gccttcggtc ctgagactgt
     7381 gccagagcag cgtctggtag gtatttgccc gttcttccat tatgccgaca atgcgcgagg
     7441 gatcgtcgcg aaagacctcg aatcctttgt cgctcttgtc gaggccgggc cctcctgcgt
     7501 cgaagggaaa tgatgtatcg atcacgatga tcagaccgct ttttgaagac cccttaggga
     7561 tttttttcaa gaacagggtc gtgagggatt cggtccccag attgtcgaag agcccgccgt
     7621 cgccgatatg caggtacgtc ggcctgccgg cgacttggta ggtgaccggg cccacgactg
     7681 gagggaagga ggcggaagtg gccacggcga gagacaatgg aagctcgcga taatcagcgc
     7741 cgatccgctc gaaggtgagc ggcagaaact gtcggctggc tcgttctaag cccttctgaa
     7801 tgatggccat gccttcaggc gtgaccggca ccgggcgatc gggcttcttt aactccttcg
     7861 tcaaaagttc gataaagtcg taatcgaaat ccgaagccgg tagtgtggtg aaggcgaagc
     7921 gccgtccgga gttatagacc gtaccgttca agatcacgcg gggaatatcg ccgcgttgct
     7981 cccgcttgta cagttgagcg aaggtcgtgt gaccgagaaa gtcctcgtcc cagacttccg
     8041 acagcgaata ggcgagcttc gtcgggttca gagcgcggaa gaatgcaagt tggcggaaca
     8101 gagcgctccg ctggaaattg gactgcatcg cggtcttata gagggaaaag aattcccgat
     8161 accgtggaga gagcccgttt acgccgagca ctggttcgga cttcggaggt ttgttggtca
     8221 cataataggc cgtggccaga ctgccgccgg acacgctcga catgtagctg acggtttcca
     8281 ggacgctgcg gttctccgtg ccaaaacgaa cgggaacctc cgccagggct tccaaggctc
     8341 cggcggcgaa tgtcgctgcc cggctgcccc cgcctgagac ggcaagccct accatgaaat
     8401 cctggtcaga caaaggggcg aacaacgctt cggcatgctc cggttgtgcg ggaagttcat
     8461 gaatcttcgc aaagcatccg gcgaggagca ggccggcgct catcatggcg acaataggtc
     8521 gtctcacgct ctgatgtctc cttttccttg ttcgttcttt tgcctcattc caaaatagca
     8581 aacgcctcct tgttcgacga gctttttcac gagttcgtct tggtataaac atgttggaga
     8641 tgagagcgca aaatcgatga gggaaccctg cgggtgcggg tagagggtga accgcgacgg
     8701 acgcccgccc atgtgggtca caggcacctc gttcaagaac gacggccagg atccttgtca
     8761 gagcctaggg cgaatgcttt ttgtctttct gcatgatctt gtcggtccaa gccagcagaa
     8821 tggccgaccc caaaaaggtc atgtggatga aaatcttcca cttgatgtgc tcagggttct
     8881 cgttcgcgac atcgacgaac gctttgagca agtgtatgct tgaaatgccg atgagggagg
     8941 ccgccaattt gactttaatc gtgccgggat ccacatgact gagccaatcc ggccggtccg
     9001 gatggccttc caggctcaac ttgctgacaa aggtggcgta gcccccgatc accaccatgg
     9061 tcaacagatt ggcgaccatc gtgacgtcga tcagtcccag cacgccgagc atgaattcgg
     9121 tttcttcaag gccttgaacg tgcaccacca tttcccacag ttccactaaa aatttatagg
     9181 cgtagagcag ttcggctacg atcaggccgc cgtacagcgg ggcttggatc cagcggctgg
     9241 caaacacgac cacttcaaaa ccgtgctcga gtgcgttggt attctctgag atggttgaac
     9301 caatgcgttg atcggacatg atgctccggt gtttgcgaat acgttcaacg gaggaaggac
     9361 ctgatggggg ctgtatccta gagggctgga cggtctctgt caaatatttc gcatgaacga
     9421 cggcaaccta cggggaacat catgattgag gtgcgtggga agagtgctcc cgcagtcggc
     9481 ttatgtcgaa ggaatggtaa gcgtgactgt cgtgccctgg ttgaggacgg atgtaatgga
     9541 gatacggccg tgaagctttc tcgctcgagc gcgcatgttc tgcaaaccca tgcccatacc
     9601 ctgcttccgc cgttgaaggg gagagaaccc gtggccgtta tcgatgactt ccagacggat
     9661 cttattccca tgtcgccgca gggtcaccat ccgcttgtcc gctttggtat gacggatgct
     9721 gttgctcagc gcttccttgg cgatggccag cacatgggtg cgctgttccg gagccagccg
     9781 caggccggcc tcgtcgtcta ccgtgacgac gatctctccg gcgcccgtcg ccatgaatga
     9841 tcccgccagc gttcgaaggg tatcggcgac ggattgctcg aaaacgacgg gaggctgcac
     9901 gtgagaaata aacgatcgca cctcacgaat cacattgttc aattggccga cagactgttc
     9961 caaccgtttg gcgtttgttc gtgagatccg ctgtatgcgt tgttttgtga gctcgagtcc
    10021 catgccgacg gcatagagag actgcaagag attgtcgtga agatcttgac tgagccgctc
    10081 tctctcctca aacgcctgcc ggaccgccac ctcgctgcgg cgcagtgcct cttccgcccg
    10141 tttctgttcg gtaatgtcag ttccgatggt caccagacat tcgttgccgt tgagcacgat
    10201 cacttccgtg gatgcgagga actggcggac ctctccacgc ttcgatttca gcggaacctc
    10261 gaggtttctg atggtgccct tggtccggag gcgctcaaca aattcctgcc gtgcctcggg
    10321 atcaggccaa atacccagga cggaggtcgt ctggccgatc acctcctcgc gtgtaaaccc
    10381 gaagacttcc aatgccgcat cattgatatc gaggcaggcg ccggtttcca agtccgtaat
    10441 gccgatcgga tagggactcg agcgaaacgc tttgctgaac cgttcctcgc tcgcgaggag
    10501 ggcttcctcg gcccgtttga ggttggattg gtcgacgtac aaaccaatgg ccgtcgaaac
    10561 gtcccctccc tccgagtgaa cgaagacggg ccacagcaag aggtccagta tcttcccgtc
    10621 cttacggcac cgccgcagtt cgatcggacc gtgaatctcc cctcgcagtc cgcgttccca
    10681 aagcgactca gccctggccg cttcttcttg aggaacgtac ggcagttcgc ggcccaagac
    10741 ctcctcttcc gaccatccga acaagcgtgt tgccgcttga ttccaactgg tgacacgggc
    10801 ggcgagatcc aggctgacga tcggaagggg agactcacgg acgagtgttt gaagcagctg
    10861 agtcgttcta gccaggccgg cctctgcctg tttccgatcg tggatgttct gcatgacggc
    10921 gatgaaatag gcggggacac aagcgccgtc gcggacgagt gagacggtca tctcgaccca
    10981 aacccagacg tcgtcgctgc ggcgataccg tgtttccatg gcgaacgagg gacgcttgcc
    11041 gctcaccaat tggcggatct gatcgagaat cagttggaga tcgtcgggat acacgaactg
    11101 ctggaacgtg caccgcgtca tcgttcttga tgaatacccc agcgtctcgc agaggcgagg
    11161 attgactctg aggaactgcc catccaaacc gagttgggcg atgccaacgc cggcatgttc
    11221 gaagagcgcc cgccagcgtt cttcgctctc ccgcatatag tcttcggtga gtcggcgggc
    11281 ggtgatgtcc tcgcaaacga ccagggtaag gatctgccct tgatggtcca tgacggcctg
    11341 cgcccgctct ttcacccaca gcgtgctgcc atctttccgc actttttgga tttcccactg
    11401 aaaaactttc atcgggctgg ctgcgcaaac ggtgagttga ccgaggacgg cttgatgctg
    11461 atccggctca aagactttca gcacggactc accgatcagc tcatccggtt gatatccaag
    11521 ctgcgtggcg ccgaattgat tgacggagac gaccgtccca tcggccccga gggtgaaata
    11581 catcgacggg ttgttgtcgt acagcagccg gtagcgagct tcgctgtctt gcagcgtgga
    11641 ttcgacttca agacggcggc gggtttcctc ttccagactg acgagcccat gactgggggt
    11701 aaggccgttt cgaacaactc cgctggctgc aggagcggca ttcaaggttg gtgtgtcggg
    11761 gaggcccgga tcaggctcat gcttgccggc tgggggcgag gtcatcgacc ggtcttgtgc
    11821 gcggcgggtc atcgatctcc tgaaagaaat aagggcgacg tacgaatcga gttagttggc
    11881 aactgagtgt tcaggggcta gcatagggcg gttggcctcg aaattccagt aggcgctgag
    11941 cgccgtacag tggagcgcgg caggtggtca cgctaattcc cggcgaagtt gctggacgac
    12001 gcgttcgctc aaccccgctt ggcgaaggtc ctcttcactg gcttgcgcca catttgcgag
    12061 gctcccaaat cgcttgagca gcttggtgcg ggtgatcgag ccaatgccga tgacctgatc
    12121 gagatgggag gtcacgagcg ttttcccacg aagtttgcga tggtacgtga tggcgaagcg
    12181 atgcgcctcg tcgcggatcc gttgtaggag atgagtggcc ggcgagtttg cacgcagcac
    12241 gatcggattt ttccgccctg ggagatacac ccgttcatct ttttccccct tcgctttggc
    12301 caatccgatt atggcaaggt cggactgtcc caattctttg agagcggcct tagcgaaagc
    12361 caactggccc agtcctccgt cgatgacaat cagatccggg cgagcgagat tctccatccc
    12421 gccgtatcgt cgcgtgacga cctcctgcat gctcgcaaag tcattcgcgc cttggacgct
    12481 ggcgatcctg aaccggcggt agtctgactt tttcatgcga ccgtcttccc agacgaccat
    12541 agacgccacc gactggttgc ccatcgtatt ggacacatca aagccctcaa tgcgcttcgg
    12601 cgccgtctca agcctcagga cccgttgcaa ttcatctccg gcctggcggt caagctcctg
    12661 gttgcgcagg tgctcggcca gcgcggctgc cgcattctct tccgccaaca gcacaagttg
    12721 atgcttcgtg ccgcgctcgg gtgccagcac gcggacggtt tcaccgcgtt tctcggacag
    12781 ccagcgagcc acgaggtcgc catccggaat gtccaccggg atcagcaatt cccgtggagg
    12841 ttgcccttcc ttgttataga actgttcaat ggacgagcga acgagctctt catccgtggc
    12901 atcggccgat tcgagccaga aaaagtcctt gcggccaatc aacaaacctc cccgcacaaa
    12961 gagcagttgc aggtcgacgg aagccccatg tcgtgcgagg ccgacgacgt cctgatcgat
    13021 ggaagcggtt tgcgtgattc gctgccgctc gagcgtgcgc tcgatcttgg aaatccgatc
    13081 cctcagcctg gccgcctcct caaactcctc acgagacgcg gcggcttcca tgtgagtacg
    13141 aagctcatca accagttctc ggtcgtgccc ttccaaaaac tgacggacct gccggacgat
    13201 tcggtgatag tccgcctgcg tttgctgccc tgtacagggc gccatgcacc gtttgatttc
    13261 aaattcgata caggcccgtt cggccgttcc atcaatgtca atggtgcagg tggcgagtgg
    13321 aaatacccgt ttgatgactt tgagtgtctc gcgcagcgcg cctgccggcg tataaggacc
    13381 gtaatagagg gctccgtcct tctgcacccg tcggacgatg gacagccggg ggaagctgtc
    13441 cttgatgggc aggcgaagat agggatactg tttgtcgtca cgcagcacga cattgaagcg
    13501 cggccggtga cgtttgacga gattactttc aaggatcaag gcttccagtt cggaacgcgt
    13561 gaccatcgtc tccagatcag cgatctgact gaccagtaag gccgtcttgg gcgtatggtc
    13621 tgcgcctttg agaaaatacg accgaacacg gtcggcgagc cgggcggcct tgccgatgta
    13681 cagcaccgcg ccggtctggt ccttgaatag atagacaccg ggctggtcgg gcaggttggc
    13741 cagtttggtt tcaactgcgg gatttgctgc agccatgcct cattctacga gtcctacggc
    13801 gtatcgggca agcagcgcta tctgagcgag cgcacgaagg aggggctcac agtcccgcgc
    13861 gcaacttctg ccaccggacc tttcatcgtg agactgaaat ccggcgcgac atcaatcgtc
    13921 agggtgcccc ccggcatctt taccgtcacc ggactggtga ccaagtggag gcggactgcg
    13981 gcgctcgcgg ccgcacagga agaggaaccg gaggcctgcg tttcgccagc cccgcgttcc
    14041 caaattagga tggtgagctc tttggggccg gttggaaccg ccaattggac attggttcgc
    14101 ttgggaaaga gaggatgatg ctcaagcttc ggacccaggg tcaggagatc ctgtttggac
    14161 caggattctc cagccggttt gaatacgacg cagtgggggt tgcctacgct gacgccggta
    14221 aatgttaatg agcgtccggc tgcatcgatc ggctgctgga tcaactcctt gaccggcaga
    14281 gtgcaaggca acgcactcgg cgaaaatgtg gcacgaccca tggcgacggt gacggcactg
    14341 gcatcaccgt gccggtcaat gtgcaaatcg atcgtcacga gcccgccttt cgtctccacc
    14401 gtaaaatgtt tcttcttcgt cttgcccgtc gcatggagat aacgggcgaa gatgcggagg
    14461 ccgttgccgg atttttccgc ttcgctgccg tccgggttga aaatgcgcag accaaagtcg
    14521 gccttggtcg agggcgcagg ggcgaggatt ccgtcgctgc ctaatcccca gttcctgtcg
    14581 cagagagcgg cgatcctggc cggcgtgagc ttaaagctca actccttagg gtccatcacc
    14641 agataatcgt tgcccaagcc gtgcccgcgg aaaaacccgt tcttcatttc attgctcctg
    14701 cggctatcgc accgagataa atacagggcg ggagcgtact gggtcaattg gcatgggtca
    14761 atagcgagtg cgaactgagc gtcagcagct agtggcgaga tggcgaggtg tgaacatgtg
    14821 aggtggacag cgccgcgacc acattacacg atgttcgcgc ccgccggccg ctcgatcaat
    14881 tcgacctcat acccgtcagg cgcatcgatg aacacgaagc ggctccccga agacgtcgta
    14941 gtcggaccat ccgtgatggg gatgcccttt tgctgcaggg cggtcatcgt gcggtcgaga
    15001 ttgtccacct ggaaggcgag gtgcacgaga tctggttgaa ccttcacggc tccgcttgga
    15061 ggaaaactgc agagctcaat caattcttca ctatccggta ctcgaaggaa ggccaggtgg
    15121 gagccccgag gcgacgtttt tcgttccagc acctcaagac caagcacgtc ggtatagaag
    15181 gcaatcgtct gatccatgtc gctcacgcgc atccgcgtgt gaaggagttt ggtcactttg
    15241 aaatcagtca tcagtcgacc atggctgtat gccggtttgg tggcggcggg ttctcatgta
    15301 tcttagcagc gacacagagt gctgactagc ggctctgccc tcttgccgga ccggcggttt
    15361 ttcgtagaag gatttccgca ctacctggaa tcaagaaatt cggcatcgga ccgatctcgt
    15421 gatagccttg cttccggtaa aattcacggg cacccttgtt gaagtccgat acgcaggcga
    15481 atagattctt tgttcgcgcg aacactaagg attcgacgtg gcggatgagc tccttccccg
    15541 ctccctgatt tctggcccag gcggcgactc cgagtaactc caaataatcg ccaagcaaaa
    15601 acttttggcg cacgattgca atgcccgcga tccggccttc cgattccgcc acgaagcatt
    15661 cacggccatg agggattggg caaaaaatcc gcttccagtc ttcgcggctg tacccgagcg
    15721 ttttccaggg atcggactcg ccgagcagcg tgatgacggt ctcgcaatcg tcagcccgca
    15781 tgggtctgat cgaccggccg ctcatcggtt cacgggtttg cacgacagca ggtcgctgac
    15841 cgtcgtcggt ttcagccctt tgcgcggcag gatgtcagcc gtaagccgtt caacgacttg
    15901 tcgggtgtgt ttccctttgc cgttcgcatg gaggacgacg atactgcctg ctttggcccg
    15961 cttttcaatg cgcacgagaa tgtgttcggc tgacagcgat ggatccggat ctccggattc
    16021 gatattccat cggaccaggc gcaggccgag ggccttcacg acatcgaccg tgacctcatt
    16081 atattcaccg aacggcggac gaaagagcac cgtgtcatgc gcatagtgtt cgctgagcag
    16141 cttgaccgga cccaggattt ctcctcgctg ctcttccgca ttgtgcatgg gcagatgggc
    16201 atgcacctcg ccgtgcgtgc ctacttcaaa aaatgcgatg ccaagcagtt gctctacttc
    16261 cgggtcgtgt ttggcgatcc accggccgga catgaaaaat gttgcgggaa tatggtggtc
    16321 gatcaaatag tcgatcaacg ccttgtcgta tcccgccccc tttctcaccg ggcacaaatc
    16381 gaaggtgagt gcaatcgtcg ggcaggcagg aggtcccgac gtgatcacct gtcccagagc
    16441 gtcggtggcg gccgccagcg tcagcaccgc catcaccgtc acaagcccga ggtgtactcg
    16501 tcgcatgctc tttagtatac ccgaaccgta gcctgcctaa atctgtaaaa tctcatcggg
    16561 ttgtgaaaca ttgacggtcg gttttcagcg ctggtactgt gcactcatga ctctgccgag
    16621 ctgggaaatt ggacgagctt tgggcattcc catccgggtg catgcctcgt ggtttctcgt
    16681 ctttgtattc gtgacctgga ctctggcaac ggaatatttg cccggtgcct tacccggtct
    16741 ttccgactcg cgctattggg ggatgggtgc ggcggccgca ctgctgctct ttttctccgt
    16801 gctcttgcac gagctgggac attcctacgt cgcgcaacgt taccgcattc caatcggcca
    16861 gattacgctg tttctttttg gcggtgtggc gcagatgcgg gctgaaccgc cgagccccaa
    16921 ggccgaattt ttgatcgcga ttgccgggcc tgtggtcagt ttcgcactcg gcgccttctg
    16981 tttgggggca gcgttggcgg cagactggtt gtcactccca ttcggagggc agggtttcgt
    17041 cgtgttgggc gggctgttgg gagtggtcaa tgtccagctg ggcctcttca atctcattcc
    17101 cgggtttccc ttggacggtg gccgcgcact ccgagcaggt ctatgggcct ggggtcgcga
    17161 tttccattgg gcgacgagcc gggcggcgct cttgggttta ggatttggtt tcctgctcgc
    17221 aagtgcgggc gctggactca tcggaggggc catctccggc atgcttcatc cctcgacggc
    17281 aagtaacgga ggatggctca tgtttatcgg cgccttcctc tttggagccg cttggagcac
    17341 caggagacag gcctcacttc gcttggcgct gaacgcgacg tgcgtgcgag acgtcatgat
    17401 tcgagcggtt gtgacgattc cgtcgcagtt gagtctgcaa gccgctgtcg atgagttctt
    17461 cgtcgcgtac gggtatggca gtttccctgt ggctgaggag ggccgggtcg tcggcctcat
    17521 caccgtccaa gacgtgcagg tcatctccca gggattatgg acctggcgga cggtagcaga
    17581 tgtcatgcga ccggcgtcgc cggagctgtt tattcagccg gaatggtcca ttatgcaggc
    17641 catggaactc atggttcgaa cgggtttcga tcggctggtg gtgatggaag acggacgtcc
    17701 agtggggctg atcacagggt ccgccgtcgc gcggtttctt caactgcaca aggcgtgacg
    17761 cctcgtgggc atcgttccct cgtcattcgg cgcgggcaat cgagatattc tgggcaatgg
    17821 caatacgcac gagatgcacc acgagcaaga atccatagac aacgcagaaa aacttaaacg
    17881 tgacgcggta aaagtccata agaatgatcg gcaggcacaa ggcaaacaga agactcgcga
    17941 tcagcgtcat gaaggttaat cgccggcaaa attcgtagga ggcctctttc ccgatgggct
    18001 tcaacgcgcg tgcatagcgt tcggcacgtt gcaaccgggc ttcgctcagg gttgaggcag
    18061 tggcgcccca atatcgcaag tagaaatacc cccccaggat cccgacccat ggcagagtcc
    18121 atatcggcca ataggcgccg cctccgtagg tctcactgct gaccagcgag gcgaaagacg
    18181 gcgcgtagag caatcccaag accaacggca gcagttgttc gtcctgcggg cgctctccat
    18241 cgggaccacc gtggatcaat tcccgctcga acagcggata gccgtacttg aggacgacga
    18301 gcgcaaccgc ggcgctcatg gttttgtctg gcacgtagcg gacacagttc tgaaaccagt
    18361 tcacgaccca ccagcccaca tgctcgcccc acgtcaatcc gaataccgat tcgaggtact
    18421 gatggaccat ctgctcccac tgaagcaccc tggcatgcag cgtttctgcg acggcaggat
    18481 tcaacgcgaa cgtggcatgt tcgtttaagg ctgcttgcac gaaggtgccg ggtaaactca
    18541 tgacgacggc acctccggca ccgaagatgg acagaaacca ggcatgggag agaaccgtac
    18601 tcttgcccgt gcgcaagtat ttttggaacc ccgcttctct ggccatccat ccccagtaca
    18661 gggcgccggt catgctgacc agcgaccagg ggaatatcac cacatccgct cccgactcgg
    18721 ggaacagcag ccaattgacg acggaattcg agagcagggc gacaatggct ccccaccatg
    18781 ggccgagcag aaaggccacc aaggcagtac ccgtcatatc gagaaacaga atgctgtcga
    18841 ggtgccggct cagcatcaag cctacatagt tgagcatcag gccggcggca acgatgacgc
    18901 ctgtctcgta catcaggaga tacgaagtgc cgcgcaacgg cttggtggaa aagaaatcaa
    18961 tgttcgccag cggttcctgt gtctggcgtt catgtgatat ggaaggcttg cgttccttct
    19021 tggtggtcaa gcggtgctgg atgtccatca atttttccat cacggtcagc agtgcggcga
    19081 tgaacccgat gagggttcca cccagcatca gcatctcttt ccccatgtct tcgggcatga
    19141 caggtccctc ttattgaggg tccattctag cgacgaagcg tagtctgggc cagaaaaagg
    19201 ctagaagcca acaggccgga aatcagccac cggcgttggc tgaaggggaa accagccttg
    19261 ccggtgagaa gctgccggct gcagcggctt gagaccgttg ttgcagcgcg tgagcgatga
    19321 cggcgccgag cagcagcagc agagccgaat agtagatcca aagcaacagg aggatcacct
    19381 cgagcaagga gccgtacaat ttggtgtaga cggtcgcata ctcgttgtag gtcacaaaaa
    19441 ggagtttggc agccacccac agaagactga acgttaacga accgatcaga gcttcacgcc
    19501 attgcgggcg ccggcgggga acgtagcggt acagtgcgcc gacagtcaga aaggccatcg
    19561 taaagggaag ggtgtaggtg agaaagacat cgtgagccgc gagcgcaatc aaatccaacc
    19621 cccagagatg cggcgcatag gcggtgagaa aattgacggt ttgcgtcgcc acatacgaca
    19681 gcaccagcat gagagccacc gcgccctgtg aggcgacggc gatggccgtc gaaatcaacg
    19741 gatgtcgttt ccaggtgctg tcaaacacga cgttcagtgc gtaatccagt tcgtagaata
    19801 ccagcgtgcc gaaccacgca aaggacagaa agacgagcca tcggacagtt tccaactcgc
    19861 tgattcgttg caactccacg gccagccgtt ctccaagcgt aggcagaaac cctttgagga
    19921 agctcaacaa cacctgtccg ccgatcacct catggctcac gataaaactg acgccataca
    19981 gcagcatgaa taccagcggg aacaatgaga gcaacgaaaa aaatgccagc gaagctgcca
    20041 ggctggcaca cccgtggcgc agaaaggatc gggtcagatc ggcaagcaag cgccaaaatg
    20101 acatgagcat cggcatgatt cccaactggc gtagcctgat caaaacggct gccctagcct
    20161 agcacgagta tagtggtcat gcacgactac agcgcgttcg aaagctatcg gagcatgagg
    20221 acggactgaa gggcaagact gacaacggga ttgggataga gacccaacag aataacgccg
    20281 gcgacggcgc aagccaggac gattgaaagc gcgggcgagg tgacgaggcg cggagagtcg
    20341 atgaccccgg cttccggctc tcgcatgtac atcaacatga ccacccgcat gtagtaatac
    20401 gccgagaccg ccgcgaaaat cagcgcgacg gcggcgagcc agctcagtcc cgcttccacg
    20461 gccgccatga agacgtaaaa ttttccgaga aaccctgccg tcggcggaat gccggcgaga
    20521 gacaccatga agaccagcat catcaatgcg gccaccggct ggcgtttggc caagcccgca
    20581 tagtcgtcga tgtcctcgcc ttcgagccct ccttttcgga acatggcaat gacagcaaag
    20641 gccccgagtg tcatgaaggc gtaaaaggcc agatagagca tcacgctggc aatgccggag
    20701 gtgccccctg attcccctgc ggcgcgcccc gcagccacca ggccgatcaa ggcatacccc
    20761 gcgtgggcga tgcttgaata ggccaacatg cgcttgatgt tggtttgaac gatggccaca
    20821 acgttgccga ggacgagcgt gatgacgcag aggaccacaa atagattcga ccaatcagcc
    20881 ctcaatcctc cgagcccctc gacgaacacc cgaagaaacg cagcaaagct tgcggctttt
    20941 gaggcaacgg ccatgaaagc cgtcacggcc gtcggcgcgc cctgatacac atccggtgtc
    21001 cacatgtgaa aggggacgac ggcaagcttg aatccgaatc cgacggtcaa aagaatggtg
    21061 gcgatcaaga gcagaggatc atcgaatcct ttcgtaccca gggcgtcggc aatggcactt
    21121 aaccgagtgc ttcccgtgag accgtaaagg agggaaatgc cgtacaagag caggcctgat
    21181 gaaaaggctc cgaggacaaa gtattttgcc gaggcttcca gtgaccgcgg cgacgcccgt
    21241 ttgagtccag ccatgacata gagcgaaagg gacatcaatt ccgtgccgag atagattgtc
    21301 agcagatcgg ctccggacac catgaccatc attccagaaa gggccagtag gatgaagcca
    21361 tagtactcac cgagatgaag gccttcggcc ttgagatacg gaagcgacat cagaatcgtc
    21421 aggccggtca cgccgtacaa cagcagtttc cagaagcggg cgtagccgtc gacgacgacc
    21481 agatcgctga acgccggcag acggagattc aggacgctga tttgcccgcc ggtcaatccg
    21541 atgcaaaggg ccaggctccc gagactgagc caggccagga gttcttttcg tgagtgcggg
    21601 gtcaggggat ccaggacaaa gacgaggcag gccgcagcca cgacgatcag ttctggaaga
    21661 atgacgatca gatcactccc gtaggacgtc atggccggcc agcctgagac tcacccgtcg
    21721 cagtcaagac gacgggtgga cggtgagtgg cggaaggatg agatgtcgga gccactcgtg
    21781 ctcgggccat cgtctccagc gttttggtca cgctcgcgtg catgcggctg accaacgggt
    21841 taggaaagag gccgatccag aagacgagca cgacgagggg gacgagtgtc ccaaattcgc
    21901 gatggtcgat gtcctgcaat cgcggcaatt ctcgcggaga cgggacgccg aaggcgatcc
    21961 gttgcaccat atagaggaga tacaccgctg cgagaatgat tccgagggac gcaatcgcgg
    22021 tcgcggcttt gctccacaga aacgtgccga cgagcaccag aaattcgccg acaaaactgt
    22081 tggtgcccgg caagccgaga gatgaaaggg taaagatgac gaagaatgtc gcgtatcgtg
    22141 gcattggttt cgccaggccg gcattgtccg atatcagcct gctgtgcgtg cggtcgtaga
    22201 tcatcccgac acacaggaaa agcgcacccg tggtgatccc atggttgacc atttgcatga
    22261 cagcgccttc gatgccctct tgattcatca caaacaagcc aagggtcacg aagcccatgt
    22321 gactcacgct tgaatacgca atcaattttt tgagatcgac ctgggccagc gccatatagg
    22381 cgccataaat aatggcggtg atcgagagcg ctgccatcag tggggtcatc atgcgactcg
    22441 cgtcgggcag catcggcaag ctgaatcgaa caaacccgta cgcccccatc ttcagcagca
    22501 cactggcgag gatgacgctg cccgcggtcg gcgcttccac gtgcgcatcc ggcaaccatg
    22561 tatggaacgg aaacatgggc accttcacgg cgaacgcggc aaaaaatgcc caaaagagcc
    22621 acatctgcag cgaggctgga taggtgacct gactcagcgc cagaatatcg aacgtatggc
    22681 cgccgtaaaa atacaggacg agcaccgcca cgagtaggag catgctgccg accagcgtat
    22741 agaggaagaa cttcatcgcg gcatagaggc ggttcggccc tccccagaca ccgatgagca
    22801 aatacatggg gatcagcatc gcttcccaga acacataaaa caggacaaaa tccagggcga
    22861 tgaagacgcc gatcatggcc ccttccatca ccagcagaac ggacatgaac gtacgcaccc
    22921 tggttgtgat cgactcccag gaaatcaaca cacagagcgg catgaggagg gcagtcatca
    22981 ggacgagagg cagactgatg ccgtcgactc cgaggctgta atgaatcggc ggtgatgtga
    23041 tccactgcac gtgctcgacg aactgcatgt ggcttgagga agaatcaaac agccaccaca
    23101 gaggaagcgc gaggagaaag tcggcgaccg tgatgaccag ggccatcgca cgcgcatgcg
    23161 tctctttgac cagatagagg gctccggcgc ccgctaacgg caggaagatc aagacagtga
    23221 gccagggaaa tccaccggtg tgcatgggcc tcacatcacc atcaataccg tgagaatcac
    23281 cacagcccct aaggccatgc cgagcgcata atgttgcgct tgtccgctct gcacgaggcg
    23341 tagcagccag cctccccagg caatggcccg ggcaacgccg ttgacggcgg cgtcgatcgt
    23401 cgccacgtcc atgcgtttcc acaatgctac ggccgcatga aaggtggggc gcacgaagag
    23461 gcggtcgtag acttcgtctg cataccattt gttcagagac agctcgtagg ccgcctgcca
    23521 gcgccgggcc cagcgttcag gcagtgaagg atgcagcacg tacgcgtaat aggcgccggc
    23581 gattcccagc aatcccatga cggtagccag gaccatgatt gccgtcgctc cgcttccttc
    23641 atggagcgac gccccgcctt ccctggagaa gaccggctgg agaaaatccg ggatgcccac
    23701 gtaccctgtg acgatgctca agacagcgag gataatgagg ggaacggtca tcgagtacga
    23761 gggttcgtgc acgtggtccc cgtggtgccg gtccccccga aatggccccc aaaacgtcac
    23821 gaacaccagc cggaaggtat agaacgcggt cagcagagcc gtcacaaggc cgaaggccgt
    23881 caacacctga ccgagaatcc ccgccgacca ggccgagatc agcagatcgt ctttgctgaa
    23941 aaagccggcg gtgaggggaa atccggcaag tgccaatgag ccgacgacga atgtccagta
    24001 ggtgacgggc agtttgtcct tgagcccgcc catgcggcgc atgtcctgct catggtgcag
    24061 agcaataatg acggagccgc aacccaggaa caacagcgcc ttgaacgcgc catgggtcaa
    24121 gagatgataa atgccggcgg cataggcgcc cagtccgcac gccatcatca tgtatccgag
    24181 ttggctcatg gtggagtagg cgaccacccg cttgatgtcc gtttgcgtca acgcgatggt
    24241 tgcccccagc atcatcgtca acgcgccgac gacggccacg acgtccctgg cggcgggagc
    24301 aacgtcataa agcggagcca gccttgcgac catgaagacg ccagcggtga ccatcgtcgc
    24361 ggcgtgaata agggcggaaa tcggcgtggg cccttccatg gcatcgggca gccacacatg
    24421 gagcgggact tgagccgatt tgccgatggc gcccatgaag aggagcaggc agatcaccgt
    24481 catcactgac acctcgagcg tcccgccgaa ggggtcaagg aggttgatga cttctcctga
    24541 gtgcgacggg atctgggcga agacgtcgga atagttcagg ctattgaacg tcgtcaagac
    24601 cagcagcagg ccaagcagaa atccgaaatc tcccacccgg ttgacaacga acgccttagt
    24661 cgccgcggcg caggctgatg gccgttcgta ccaatggccg atcaacaaat aggaacacag
    24721 cccgaccgct tcccagaaga cgaacagttg caagaaattg tcagcgagga ccaacatgag
    24781 catggagaag gtaaacaacg caatatagct gaagaagcgg gcatatccag gatcaccatg
    24841 catgtacccg atcgtataga tatggaccag cgtgctgacc gtggtgacta acagcagcat
    24901 gacggcggtg aggcgatcga tcgtgattcc gatgtggacg tcgagaatgc cggaggtcag
    24961 ccaggtgtag aggcgaaggg aggattcatg ccccccggcg acctccatga acgcaacagc
    25021 cgacaggagg aatgagccaa gcacggctgg gaccgccacg aaatgggcct tgtccctgat
    25081 ccaatgtccc agcagcccta caatgaggaa cgaggcgagc gggagtagcg gaatgagctc
    25141 gtagggcatc gtcaccactt caacagctga aactcgtcga cattgatggt cgatttcgct
    25201 cggtgcagcg caatgatgat ggcgagtccg acggcgactt cagcggcggc aacggtcagc
    25261 gcgaagaaga cgaagacttg gcctgcgatg ttgtgaaaag cctgtgagaa tgcgacaaaa
    25321 ttgatgttgg tggcgttcaa catgagctca accgacagca agatgatgat gacgttgcgt
    25381 ctgacgagaa cgccgaggag tccggtcaga aagaccaccg cgctcaatgc gacgtagtag
    25441 gagagaggta ccgtcatcga tcacccgccg tcacgccaat gtctcacgct tgtcgtcctt
    25501 caagatcctt cttggccaga atgatggcac cgatcattgc gaccagaagg atcaacgaag
    25561 ccacctcgaa tggaaacagg taggtcgagt agagcacgtc acccaaggct tcggtattgc
    25621 caagtccggc ggcttcgctc tcctgtccgg tccggacgac ggccgccgtt ccccccttga
    25681 ggagcgtcag cacgatggct tcgatggcta aaacagagcc gatcgcggca gccaccggcc
    25741 actgccggtg atagcggtcg tcctgcctca aattcagcag catgacgacg aacaagtaga
    25801 gcacgaggat ggccccggca tagacgatga tctgaacggc ggccaggaat tcggcgtgga
    25861 gggtcacata gagacctgcc acatggaaga acatgatcag cagcgagagc gcgctgtaga
    25921 tgggattgcg catcgcgacg accagcaccg atgacaccgc aataaccagc gcaaaataaa
    25981 aaaagaagac ctggtccata tcaacccggc attcccgtta gcgatgaacg cacatccctc
    26041 ccgtcccgac agcccgcgtc cgttcggtcg gccatggctg caattcaatt cggtttctgt
    26101 gggacgtggc cgaatgagac gttgaaaaac gccacgttgg ggtgttgcag ttccagccgc
    26161 ttctcgcgaa ctggaaaggc acggtccccg attgccagca gctgctcctt gttcaagtgc
    26221 agctggcgct tgtcgtagac cgaccattcg aattcgcggc tcatgccaag cgcatccacg
    26281 gggcaggcat cgacgcacat gccgcaaaac aagcagcgcg tcatgtccat gtaatattcc
    26341 ttggcataac gcttcgtggg ttccgccggg acttccgcgc tcacgacgcg gatgaccctc
    26401 gaagggcagg ccgcctcgca gagatcgcag cccacacact tttctgtccc gtcgtcgtag
    26461 cgcaacagac cgagcatgcc gcggtagttg tccgggagag tgcgtttctc gtgagggtac
    26521 tgcaccgtaa tcggcttata gtgaagcaag tgcgacatcg tcgctttcat gccgacgagt
    26581 atctcgtaga acacgatggt tttgaaccag gcaacaagct tcttcatctg cgccatccgt
    26641 tcggtcggcg ggtcaccgct tatcccctcg gaaacaaata aacggcaatc gaggtgacga
    26701 cgatgttccc taacgcaatc ggcagcatca ctttccatcc gaatttcatc aactggtcgt
    26761 aacgcagacg cggtaaagtg gcgcgaagcc agaagaacag gaagaggaag gcataggcct
    26821 tcactgtgaa ccaagccaga ttttcaatcc aggccacctg ttccaggccc acgtgcgcga
    26881 ggatcgtccc tggatagggc gcattccagc cgcccaaaaa catggcggcg gcgacgcagg
    26941 ataccaagac catgttggcg tactcagcga taaagaagaa ggcgaagcgc atgccgctgt
    27001 attccgtaaa aaatcccgcg accaactcgc tttccgcttc cggcaagtcg aacggcacgc
    27061 ggttcgtttc tgccactgcg gatatgacat acacgacgaa cgcaaagatc tgaggcgccg
    27121 gcagggcgaa cacgaaccaa tgccaaaagc cgcccgcttg ggcctcggtg attttcacca
    27181 ggctgagtga gcccgcaaga atgagaacgc cgacgatggc gagaccgacg ttgagctcgt
    27241 agctgatgac ctgggctgcg gatcggagcc ctcccaacag cgaatatttg ctgttcgagg
    27301 cccagccacc cagaatgatg ccgtaggcgc cgatagacgt gaatgccagg atgtagagaa
    27361 taccgatatt gatgtcgctg atgacgaacg gctctatcgt gatgccgaac agttcgactt
    27421 tatgacttgg tccgaagggc acgacggcga atccgatcag agccggtacc atcgccagaa
    27481 tcggcgcgag actgaacatg aacttgttcg cgccggccgg cacgatgtct tccttgaaga
    27541 acaacttcaa gccatcggca atcggctgca ggatcccgta agggcccact tccatggggc
    27601 ccatgcggtc ctgcatccag ccgagcacct tacgctcggc cagggtcaag atcatgaccg
    27661 tcagcatgac gacccccatg acagcggcga tttgggccag cgagactgcc agtctcaaac
    27721 tcagttcagt cacgatcgtg ctccttactc agatgcttac cgcggccttg cacgccccaa
    27781 aggcacgtcc cgggacatcg attgccaagc aatcggtggg ttgggtgggt gaaaacaacc
    27841 gccagggtag gcgggtgaga atgtgcctgt tgaacagtgg tactcacttt ctccttgtca
    27901 cgcaatcttg gtgatcgtga ctgacgtggt ccggaaggcg gggactttgg tcgccgcgtc
    27961 aatcgtcaag tcgaaaagct ttgtcgcctc ttgagcaaaa tgatcgggaa accagatcgt
    28021 tccctgcgga acacgttcca cgagtttgac ggatgtcgtc atctcgcctc gtgaattcga
    28081 caaccggacg cgatcaccgt cagcgagggc caaacgtgtc ccatctgccc ggttcatgcg
    28141 gatggcgtcg cgggattcga tctcaagtaa tcccttcgat cgggtggata gtttccccga
    28201 gtgaaacaag ctctgagtca ggtgcatgga ccaatcacct ccctgagggt gcggtccacg
    28261 cggggccaaa gtataccgag tggagaggtc atcgcgatag ccccccgaga cgtaacgatc
    28321 gacagctccc cggtccactt tttgaggagt gggtgtcgga ccgaggaggc catagcccgg
    28381 aatgacgctg cggatctctt tcagcaggtc cttgctgtcg ttgtactcca ttggtgtccc
    28441 caacagcaga gagagcgcgg acaatatttc ccaatcgggc cgactgtctc ctgcgggctc
    28501 gaccgcgggg cgtatggctt gaacatggcc ctcctcgttt gtccaagtgc cggctttctc
    28561 aaggggagcg gcggcaggga acaccacgtg ggcgagggct gccgtttccg tcagaaagag
    28621 ttcctggcag atcagcagct ccaaactctg gcaggcttct cgtgtgccga ggtgcgatgg
    28681 caaggtcccg atcgggtttt cgcccatcac aaacatggct ttcaagtggc cggtccgggc
    28741 acgttcgatc atgtcggtca aggtcgcgcc aggtgtggag gggatgtccg tgttccacgc
    28801 gcggccgatc ttcgctcgtg cgtccgaact ggcgaggtcg agtgcatcag gcaacaattc
    28861 cgccaccgct cccagctcta ctgcgccctg atcgttgttt tcttcggcca atggagcaaa
    28921 gccgcagccg ggttcggtcg atttccccgt gagcagcagc aggtccagaa gctgaaggca
    28981 ggcaccataa ccacccacgc ttcgcaacag gtcctggcca gtgagcacga tgaagcgatg
    29041 actttgggcg atcgcggctg ccatctgttt gaaagtgtcg gggctcgatc ccgtcgcggc
    29101 ctcgagttca gtccaagcga ggcgttcaag cagctgtgac agcgcggtca catagtcggg
    29161 cgattccttc tgtacggaag gggcgacatg gccgccctcc atcacggctt tgaggaggcc
    29221 gatcaatgcg ttcccgaact gtgccgtcgg cacgcctaca tggtgatccg caagattcgt
    29281 gatgttgctg atggagtcga tcccaggttg cattaactca atcgtcaaga ggcgggccat
    29341 gtgtttcttg accgcttcct tgaccctgag ccccgtcacg gggttggttt ccgtgatgct
    29401 tgtgccgacg aggagaagga catcggcctc gacgatgtcc tcaaaagtca ccgtccagcg
    29461 gtgggtacct tgaacgcgcc gcatcgcctg tacgccgttc aggtgcccgt agcgtgcgct
    29521 gctgtccagg ttgttcgtgc cgatcaccac tctcatgaac ttttgaaaca agtagagatc
    29581 ttcgttcgta cagcggctgg acatcaggcc gccgaaagcc tgggctccat ggagccgctt
    29641 gagccgcgca atttgctccg ccgtgtactc gagagcctct tcccacgtcg cttcgacgag
    29701 gacgccgttg cggcgcacaa gcggcttggt cagccgatcc ggccggctcg tggcatgaaa
    29761 cccgaagaag ccgcgcgcgc aaagatcgcc gttgttgcgg cccgccccat aggatgaatt
    29821 gacctccacg agctcgttgt ctttggtctg cacggtgatc tgacagccgt caccgcaata
    29881 ctggcagatg gtatcggcgc gcttcagcat ccagggacga tattcgtaca tggacaagcg
    29941 actggtgatt gcgccgacgg ggcagatttg cacacagccc ccgcaaaact cgcagtccag
    30001 cggatgggaa ccgaaggcct tgatctcggt catggtgccg cggccggaag gggccagggc
    30061 tttgacgtcc atcacctcgt cgcaatagcg gacacaacgc aggcattgga cgcagcggtt
    30121 catctgcgtt tcgatgaggg gactgaagta ctctttctgg aaaatgcgtt tggtttcgag
    30181 gaaacggctg ccggtagccg tgtattcgtg ggaaaagtct tgaaggtcgc acttgccgcc
    30241 ctggtcgcaa acgggacagt cgagcggatg gttggccagg atgaattcga gcaccgattt
    30301 gtgcgcatca ttcacgacgc tggtggccgt ccgcacggac atgccatctg tcgcgatcgt
    30361 actgcaggag gtctgcagct tcggcatctt ctcgatttcg acgaggcaca tgcgacagtt
    30421 ggcgtccggc ttgagtttcg gatggtagca gaagtggggg atcatgactc ccacacgccg
    30481 ggcagcttcg atgacgagcg tgcccttcgg cacggtgatt tccatgccgt caatcgtcaa
    30541 ccgaatcgtg ggtgaagcgg acgcggtcat ggcttaaccc ctggccccta ctggctgcgg
    30601 acggagcaga ttcgcggctt ccgcctcgtg gatgagcgcg atgtattcag gccgccaatg
    30661 ttttaacgta ctcatgatcg gggcgacttc ggcgtctccg aaggcgcata cggtgcgacc
    30721 ggcgatgttc ttacacaggt ccgtcaaggt ttcgaggtcc tgagcccggc ctcgtttgtt
    30781 cacgatccgg cgcattgtct gtaccagcca ggagctgccc tcccggcatg gactgcactt
    30841 gccgcaggac tcgtgataga aaaattccat caggcggagc gcggcccaca ccatgctggt
    30901 gccttcctcc atgacggtga cgcccccgga acccaacatc gaaccagctg ctgcgaccga
    30961 ttcgaaatcc atctttacgt caaggtgatc gggtgtcaga aagggcgccg atgcaccgcc
    31021 gggaataaac gctttcaacg gtttctcgga ccgcattccg ccagcgtgtt cataaattaa
    31081 ttcccggaac gtaatgccca ttggcacttc gtaattgcca ggtcgcttca cgtgtccact
    31141 gacacagaag atgcgcgtcc ccgtgctctt gggcggtgac ccgattgctg caaaccattc
    31201 agaaccacgg ttgatgatat gaggcaggtt ggcgagcgtt tcgacgttgt tcacgacggt
    31261 cggtttgttg tacagcccat gcgtggcagg aaacggcggc ttgacgcgtg gaagacctcg
    31321 tttcccctcg agcgattcca gcagcgcggt ctcttcgcca caaatgtagg cgcctgcacc
    31381 gcgatgcacc cagatatcga ccgtcacgcc tgtgcccagg atattcttgc cgatgtagcc
    31441 ggcccgacgg gcttcgccaa tggccttctc aagaattcgc gcaccgagca cgaactcgcc
    31501 tcgaatgtag atgtaggccg tttccgctgc gattgcgtag caggccagca cgattccttc
    31561 gagaatttga tgaggatcgc gctcgatcaa ctgccggtcc ttgaacgtcc caggctcgct
    31621 ttcatccgca ttgcagcaga gataccgcaa cccttggtaa tctttgggta gaaagcccca
    31681 tttcactccg gttggaaatc cggcgccccc acggccacgt agcccggact taatgactag
    31741 ttgtgtcacc tccgccgggg acaccttccc catgaccttt ttgatcgcct ggtagccccc
    31801 cgtctgttca tagtcggcca acgagccggt atacccgggc tgactcatat ttttgaggag
    31861 gataggttcg tgctgtgcca taacttcagc gtatcggcct ttcgcttttc atagcaagag
    31921 gctctggcca cataaagggg ccgctcttga gagcggaaga gccagtggct ttgaggtctg
    31981 ccacgatccg gtcgaacttg tcttcggtca ggcgttcata atagtcgtca ttgacctgca
    32041 tcatcggtcc ggttccgcag gcggccagac attcgacaag ggtcaatgtg aatacgccgt
    32101 cggctgtggt ttcgcctgga tcgattccaa gcttgctttt gatccaggcc accacggtat
    32161 cagagccgac gagcgcacac atgagtgatt tgcagacctg gatatgaaac ttgcccacct
    32221 gtttcagatt cagcatggtg taaaatgtgg ccgtctcata gacttgcggg ggcgtcaact
    32281 tcagcaggga ggcgatttcc gtcatcgagg cttcggtgat gtagccgtgc tcgcgctgcg
    32341 ccaggtagag caagggaatc aaggcagacc gcctgacggg gtatcggctc agaatttcgt
    32401 caatttcccc ttggtacttg tctcttaaca tgtgccgttc ctaaatcggt gaagttggag
    32461 ccagtgtgtt gctaaaactg gtccccgaga tcgcgcgttt tcgttggggc gagggctgat
    32521 tgatctccta ctgcgtggct cgttagcgtc accgatcaca ctctcccatc acgatgtcat
    32581 aggtgccgaa aatcgtaatg atgtcggaga tgagatagcc tcgggccata tggtcaaagg
    32641 cacccatgtg gacgaaggac ggagcacgga tcttcaaacg atagggccgg gcgctgccgt
    32701 cgctcacgat gaaaaaccct agctcgcctt ttggagactc cgacgcgcag tacgtctccg
    32761 ctttcggcgg tttgaagcct tgagtaaaga tgatgaaatg gtgaatcagg ctttccatgt
    32821 cccgcatcac gagctgcttg gggggcggaa tatattgcgg cgcatcggcg acgattggac
    32881 cctgcggcag ttgatcaaga cattgtctga tgattctcgc gctctgtcgc atctcttcca
    32941 tgcgaaccca atatcggtcg taggtgtctc cgtttttgcc ggtcggcact tcccaatcca
    33001 ccttgtcgta gacgccgtac ggctcttctt ttcgaacgtc gtaggcgacg ccggatccgc
    33061 gcagcaccgg cccgctgcag ccgaaattga tggcatcgtc cgcagagatc acggccacgt
    33121 gcttggtacg gccgagccag atccggttcg aggcaatcag ggaatcgtat tccttcacct
    33181 tttcaggaaa cgcatcgagg aaggacttta accgcaggac cagatccgga gtgaagtcgc
    33241 tgtcgacgcc gccgatgcga tagtagttca acgtcagccg ggcgccgcag agcttttcaa
    33301 acatgtccaa caaggcttcg cgttctcgaa acgtccagaa aaacaccgtc atggcgccga
    33361 tatccaacgc ctgggttcct agccaaaaca gatggccgac gattcgctgc atttcagcca
    33421 caattgtccg gatgtattcc gcccgttccg gtatcgcaat gccgagcagt ttctctactg
    33481 cgcgtacata ggcatagttg ttcgccatgg cgcacacata gtcgaggcgg tctgtgtggg
    33541 ggatgacctg catataggcg agtccttcgc agagtttctc gacaccacga tgcaggtagc
    33601 ccaggtccgg ggtcgctttg acgattcgct ctccatccaa ttcgagcacg acccgcagga
    33661 ctccatgcgt gctgggatgt tgagggccca tgttgagcaa cagctcttct cggcggcggg
    33721 agcccgtcgg cgaatcaatc ctgaacgctt ccttctgaga ttcgggaacc tctccctcgg
    33781 ccagttcaac cggaggttcg tctagtcggg ggatgaactc aaattggctg cgccagcccc
    33841 gaccctccgc agggaaatcc tttctcagcg gaaacccttc cgcataatcc tccggcaaaa
    33901 ggatgcgtcg taaatcggga tggcccgcaa aactgatacc catcatgtcg tagacttcgc
    33961 gttcgagaaa ctcggcgcct ttccagatgc cggtcacaga ggcgatggtc ggctcctcct
    34021 cggacacgcg ggctttgagg cgtatgcgac ggtggtaagt tagtgaatgc aggtgataga
    34081 cgacttcaaa gcgctgccga tcctccgggt agtccacgga gcaaatatcc gtgagatggt
    34141 cgaacgatgc ttccggcgca tcgtgaagaa agcgagccac atcgagaatc cgatcggcag
    34201 ctacctgaac ggtgacctcg gaccgcgccg tgtccgcctc gacgcagagc accgcctcgg
    34261 gaaatttcgt catcaacgtt tcgatcaggg gctgcatgac ttatctgacg aagacctttt
    34321 ctcgctggat cttttcctgg agcttcatga ggccgtccat gagagcctcg ggccgaggcg
    34381 gacagccggg cacatacacg tcgacgggaa cgatctgatc cacgccctgc acgaccgcat
    34441 aactgttgta atggtttcct gaagtggcgc acgagcccat ggagatcacg taccgcggtt
    34501 caggcatctg atcgtagatg cggcggatga cgggcgccat cttacgcgtc accgttccgg
    34561 ccacgatcat caagtcggat tggcgcggag aggcgcggaa cactcccgcg ccgaaccggt
    34621 cgatgtcata gcgtgacgac acgctggcga tcatttcgat cgcacaacaa gccaggccga
    34681 acgtcatcgg ccacagtgcc gattttctgg cccagttcac gaccgcgtcc agattggtcg
    34741 tgatgatatt cgcctcaaac tgtcgctcta aaaaactcat ctttatcttt ctctctccac
    34801 tcgtctttct tagttgttct ccatcttcag aggtggccct ttagcctcac acgtgcgcgc
    34861 gtgaacgagc acctcccgca atgtgcaaac aagattggtc cccgtgtgcc gcgttccgcg
    34921 agcacaagga ggctaacggg ccaccctgcc tcttcccaca ccgggccggg tgcgaagccg
    34981 tctgggccga tcgcctgcgg gacccgcctg atccccgctc aatcccattc taacgcacct
    35041 ttcttccatg cgtaccagag tcccaccagc aaaatggcga tgaacaccaa catttcgacc
    35101 aatcccaaca gaccgagcga ctgaaagcgt acggcccatg gaatgatgaa aataacttcg
    35161 atgtcgaaaa tgacgaagag catggcgatg atgtaatacc gcatcgggaa ttgcacccgg
    35221 gcatcggaaa acaacgggct tccgctttcg taaggattca gtttggcacg atatggacga
    35281 tgcgggcgga ccaggcgtcc ggccaagagc tggatggctc caaaggcaat cgtaatgccg
    35341 atgaagagga ggatcgggag atagttcagg ggaacggctt cgtccgccat acacgtctcg
    35401 ctactaggcc gtcacatgcc ggctcaacgc cgagccaaaa aacggccgga aggtcaatcc
    35461 atcgagtgcc ggtcggtcca gtgccttgtg ttgcacgaga atcttgaagg ttcttcccat
    35521 accctcgggc ttgagcaggt ggatggccgc gcgaaattca gcactgtccg gcgccaattg
    35581 ggccatcatg tcttcaatac ccaatcccat cagaaaactc atttggttgg tgaaaccggt
    35641 gacggacaag ccggactgct cacccaccgt agccaaggtc gtaaaatcca cgtgagcggt
    35701 gatgtcttgc tctcccacgc ggacgtaggg atcttcgcct gccatttgcg agtgatagca
    35761 caggagtgtg cctcgcgatc gctccggtcc ataaagatct tcggcggcat gcccgtaatc
    35821 gatcgtaatg acgacgccct gctcgaggct attggccacc tggctcatcc agcctggcgc
    35881 ccgaagattt acttccgtcc gatacccttc aggaagctcc acatggatgc gttgcaggta
    35941 ttctgcgata gccttggtcg acaggccttg cagccgttcc acgaaaccgt catcccgaac
    36001 gtccacgaag acttcccaaa gcgcgtggtc ctttttttga acccggtgga caggaaaggc
    36061 atcggctaat tcgttgctga acatgaggcc gcgcacgctt tcctgcgcga gactgccgac
    36121 gtcttcgacc cagctcacgc aaccgggctg ctcgagccag agttcgaggt gtcgttgctg
    36181 catggcgcgc atcgccggac tgcattcgat caggacgtat ctcagacggt gtgccaacga
    36241 ctgaggagcc agttgcaggc ccgacaacac atgactggcc aggaggcctt tgcccggtcc
    36301 catttccacg acggtaaaag ggtccggata tcccagcaaa gcatcaattt gtgctgcttg
    36361 tctggcgatg gcttgtccca ggatagggtg aacgtctgag cttgtataaa aatcaccatt
    36421 ccaaccaatc cgctcaacac cggcctccgg gaagcgcatg taatagccga attgcggatg
    36481 gtagagggca agctccatga agcggacgaa agggatggga ccctcacgtg cgatttcgga
    36541 tgcaatggcg gcgacgagtt gcggatggcc ggtgctcacg aagaactccg gaaaacacca
    36601 taatgatagg gacaccccga tcccatgggt tgtcttgtac ccataagatt gggacggtcc
    36661 aaaagtggcg tagagtagcc tctaagagtg cgctaagtca aggtggaata agggggaatc
    36721 gcagcccatc gctcgttccg ggccgccaat tcgaccgctt ccttcctcaa caactgcttg
    36781 acgccgaaac tcgtttcgtc caacgcattg cgggacgaaa cttcagacat cggcgtcacc
    36841 tccctctggg tagggcgcag ttgtcgtcgg aacggtgccc caattgccgg caagtcacct
    36901 cgcaacgtct gcgatttccc tttctctcta accagggccg ctgcgacagc gttatcgacc
    36961 ttcgcgtcaa ttcgggaacc gttcaatcga acagttgcgg ctcgctgaaa gcgagtggtg
    37021 tcgatgttcg aagttccgtt catgcggagc atgaaaggga acaagtttcg atgccaagca
    37081 gctgtgagat tagaacggtc ggtcggattg acgcgtggga gagagatggc gccgcggccc
    37141 taactacgcc ccgtggcatt tcttatactt cttgccgctt ccacagggac agggatcgtt
    37201 gcggccgact ttatcgtcgg tgcgatggac cgttggcgca gacaccggtt cttccccacg
    37261 atttaatgtg agcttgggct gcggccggga ctggacgggc gggggcggag cggaccgttc
    37321 gccttcccga acggcctgca cgaggaagag ccgttcgatc gtatccgact tgatgcgttc
    37381 catcatgccc gcgaacagat cgtatccttc ccgcttgtac tcgatcagcg gatctttctg
    37441 accgtagccg cggaggccga tgccgtcgcg gagatggtcc attcccaaca gatggtcctt
    37501 ccagtggtgg tcgatcacct gcagcatgaa attcttctcc aggaagcgca tgagctccgg
    37561 cccaagctct ttttcctttc ggtcgtaggc gtcgcgtacc tgggccttca gatcttcaag
    37621 gagcgcgtct cgtcccacgt cacggagaga ttcaccgccg tcctgcttgc cctgcgtgat
    37681 gtcgagtccg aactggccct gcatcatctc ggccagacct ttcatgtccc attcttcggg
    37741 atattgttcg gcgggacagt acacagtgag cgcggactcg accagggttc ccatcatttc
    37801 gcgcaagtcg tcgctgaggt tttcgcctgc caagaccgca cggcggtgtt gataaatgac
    37861 ctcccgctgc ttgttcatga cgtcgtcata ctcaaggagt tgctttctga tttcaaaatt
    37921 gtgggcctcg accttctttt gggcattcgc gatcgcgcga gtgaccatgc cgtgctcgat
    37981 cgggatgcct tcctccatgc cgagcttcaa catcagttgc gagacccgct cggacgcgaa
    38041 aatgcgcatc aaatcatctt caagcgacag atagaaccgg gaagaacccg gatcgccttg
    38101 acggccggcg cgcccgcgca gctggttgtc gatgcggcgg ctctcatgcc gttctgtccc
    38161 gaggatgtgc aacccgccga gagcgaccac ctcctgcttg tccttttcgc agtcggcacg
    38221 gatctcttca tagacggcga gctttcgttc ctccggcagc tgttcctccc gatagagcac
    38281 ctgcttgaac atgaagtcgg cattgccgcc aaggagaatg tcggttccgc gaccggccat
    38341 gttggtggcg atcgtcacgg cacctttccg tccagcctgc gccacgattt cagcttcacg
    38401 ctcgtgctgc ttggcattga gcacgttgtg tttgattccg tttcggctca gcatgcccgc
    38461 gatcttttct gatttctcga tcgagattgt gcccaccagg accggttggc cccgttggtg
    38521 gcagtccttg atctcttcga cgattgcggc gaatttttct ttttcagtgc ggtagaccac
    38581 atcggcgtag tccagccgaa tcatcttcct gttggtgggg accacgttca cgtcgaggtt
    38641 gtaaatcttc gcaaactccg ccgcttcagt gtcggcggtg ccggtcatgc cgccaagttt
    38701 cttgtacatc cggaagtaat tctgaaaggt caccgaggcc agggtctggt tctcgttggc
    38761 aatcttgacg ccttctttcg cttcgacagc ctggtgcagc ccgtcgctcc agcggcggcc
    38821 cggcatcaac cggccggtaa attcatcgac gatgatgact tcgccgtctt tcaccacata
    38881 gtcgacatca cgcttgtaga gcgcataggc ctgcagcgcc ttgacgacgt gatggacgag
    38941 gtccatgttg tccggatcgt agaggttgtc gacgcccaag agcttttcta cgcgcacgtt
    39001 gccctcctcg gtgagggcgg cggtcttcgt tttttcttcg attgtatagt cgtgctccgg
    39061 cttgagctgc ggaataatgg cgttgatgcg gtagtagaga tcggtgctct ggtccgtggg
    39121 tccggagatg atcagcggcg tccgtgcctc gtcgatgagg atactatcga cctcgtcgac
    39181 gatcgcgaaa ttcaggtccc gctgtacgca ttgatccaat gcggtcacga ccagattgtc
    39241 gcggagatag tcgaagccgt actcgttgtt cgtcccatag gtaatgtcgg cgcgataggc
    39301 ctcgtacctg gtacaaggac ggaggtgctg gaggcgctta tcggcggcat cataagccgg
    39361 atcgtagatg aacgaggcgt cgtgctgaat gactccggtg gatagcccga gcgcatgata
    39421 gagctggccc atccattgtg catcccgttt ggccagatag tcgttgaccg tcacgagatg
    39481 aactccctta ccttcaaggg cattgaggta aatggggagg gtcgcgacga gtgtcttccc
    39541 ctcgccggtt ttcatttcgg agatccgccc tttgtgaagg atcatgcccc cgatcagttg
    39601 gacgtcgaaa tggcgcatgt tcagcttgcg ccgagacatc tcgcggcaga cagcgaaggc
    39661 ttccggaaga atgtcgtcga gggtttcgcc ggcggccacc cgcttcctga aggtctgagt
    39721 cttgtcggcc aaggcgtgat cggaaagcgg aatcaggtca gattccaaac cgttaatgcg
    39781 ctggacaatg ggcatcaagc ccttgatttc ccggtcgttc ttactgccaa aaatgaagtt
    39841 gaggatctgc gtcagcatcg ggatctacag acaaaaaacg gtgtgaacag ccacgtgatc
    39901 caggcagtat accggaaaaa ttttccgaat cctatcggga agacaagggc ttccgatact
    39961 cgggcttgac gctggaccgt gggtttggct accatcccaa cgtcggactt gtgtcgttgg
    40021 gcctatgatg tcttggtcgc cgaagcaatc gcttgccgtt cttctcgtcc tgagtctcct
    40081 cgtcatcgga ggactgggct cggcgcaggc gatctctcac gaatctcacc acgcccatca
    40141 tcaaaaaacg acgcatgcga cagtcctctg cgcatggatg tgcgcggcag ggcaggtcct
    40201 cgacacggtc accgctccag atctcattga acgctccccc gtctcgcgcg ttgaattttc
    40261 agcgctgccc gaatggtcct ccggcttctc ctctcttctc tcttcccgcg gtcctccgac
    40321 agacatcacg atatagttca acgtaatcgt gtcaagcgtt gcggtcgcag tgtgcgccga
    40381 tctgcagcgt cggatagagt gctccctttc tcgtggtgtc cgtatgacgt ccataggtca
    40441 gaacagattt cttcttcccg cctggggttt ttcgctgctg ctgcacggcc ttgcggttgt
    40501 gctggcgatg atttttgccg ctcagatcaa gcctgtcctg aaagaagagg tattcaagtg
    40561 ggatgtggca ctggttgacg cagtgagcac tgaatccttg cctgaatctg ctcagccggt
    40621 ggcccctcaa gctcaaagca aaccggtcgt gactcggccc tccagatcaa cacgggagac
    40681 cgcgttgaac cgggttgccc cgcatcagtc ggtagctatg gtccatcccg aaacccagac
    40741 gggcaaaccc agtgaacaga aagccgatct cattacgccc ccacaactcg agcgggttga
    40801 accacagatg gcagaggtac gtcctcagac acacgagccc atggaacaga aggctgtgga
    40861 ggtcccgaag cccgaccctc aaccggttct tgagccggag aagccagagc cagctcaaaa
    40921 gaccgagccg gtcatcgcac agcgtcaggc cgttgcgccc ctccctccgg cggaagtgaa
    40981 agaggatcac gtcgcccaac cggctgtcag tgaaactccg gccgtcgagc cagtgcgaga
    41041 agcgagcatc cagaacccgg cccagcctgc gacatccgtc gaaccgacca cagcctcgcc
    41101 gccgaaggtc ggtctgccag aggcgccgag tcaggcggct caggcgactc cgggtcctga
    41161 taccaagctt gatcggtggg tgggcgagtc cctgtggcgg cgggtgggtg aactcaaacg
    41221 ctatcccgcg tctgcccgtt tgaacggtca agaagggaag gtgatcttga aagcggtcat
    41281 acgagcggat ggacatctgg cagaggtatc cgttcaaaag agttccggcc atcacatact
    41341 tgatacggct gcgatggaag cggttcggct tgcctgccct ttacatatga aacatgccat
    41401 cagcaagtcg gaaatcgtcg tcaggcttcc catcgtctat agtttggcga actaggcgaa
    41461 ggtggagggt cataccgtga aaaagggatc agcgaggacc aaccgcctct ccatccagcc
    41521 gttacacctc attgtgggcc tgatcgccct gtggccaatt gtcccagcct tggccgagac
    41581 gcaacatccg ccggcgagct ttggcgcgaa acatatgtcg gtccaccatt caaagacggt
    41641 ggtgggtccc tcggtgcaga ttgatgagca aggaatcatc tttgccgcct gggtggagga
    41701 agacaaggac acacgtacga ttctattcgc gagatcggaa acgcccggtg gaccgcttgg
    41761 aactccggtt ccggtcaatc ggccgagtga gagtccctat tatcgtcagg aatcgcccgc
    41821 ggtggcggtt cgcggaggcg aggtcttcgt cacctggtca atgacccatc ccaggaccac
    41881 ctctgacaaa ccgttttcgg gagaattgag attaagccga tcgatcgacg gcggacgctc
    41941 gtttgcccct tccacattgg ttaacgacga tgaacaggtg attcagcacg cctttgatgc
    42001 gatccatatc gcccctgacg gggcggtgca tctggcctgg atcgacgggc gggaaggcaa
    42061 gaaggagccc ggaacgttcg tcgcgagatc gacggatcac ggacgcacca tcaccaagaa
    42121 tttcaaagtc gacgacaaca cgtgcgtctg ttgccgcaca gccgttgccg cttcggccga
    42181 cgcggtgtat gtcgcctggc gcaaaatttt cgatggcaac gtccgtgaga cagtcgtcgc
    42241 cacatcaacg gacgggggag agactttttc ttctcctgtc gtggtggggc atgatcagtg
    42301 ggcgtatcag gcctgtccgc accgccccgc gtcgttgggt gtggacgcgg cggggcggct
    42361 ctacgtcgta tggtacacgg aagggatgga tgagacgccc gccatttatc tggcctactc
    42421 ggatgatcgg ggggtcacct tttcggaaaa gaagcagctc aaccgctcaa aaggtacgtt
    42481 tcccgatcat ccacagttag ccgtcgatcc ggaaggacgt gtagtggtga tttgggaaga
    42541 gcagtctccc gtacgtcgtg agatcgtgat gagtcattct atcgaccgag ggcaaacatt
    42601 cagcgttcca ttcaagctga acgagaagaa tgggcagacc ccgaccgttg ccgtcaatca
    42661 acacggcacc gtcgtcatgg ggtggaagga acacgcgatg ccggcgcatc gtctcgtcgt
    42721 ccagatcatg cagttgccgg ccgtggcagt agccgaatcg gagatcatca gtcatgggcc
    42781 gtaagcgata ccgatcgttc ctcctggcac tcatcgccgg atatgcggtg acgttcggtt
    42841 tcgacgcttc tctgtctggc gccgatccat cgaccgaccc gttcgtcgcg ctccgtgtga
    42901 gtcgagtgcc gacaggcaat ccactcgaga cgttcgacct gaaggcgctc gatggaggaa
    42961 cgcttcgctc ggcggatctg atcgggaagg tcgtgctctt gaatttttgg gccacttggt
    43021 gcgggccatg caaagaggaa atgccatcgc tcggccggct gcaggagcac tttgatccgg
    43081 ctcgtgttcg cgtcgtgaca gtcacgacgg acatgtatcc tcagggcatc cgacaatttc
    43141 ttgatcaact ggctatccgt ctgcctgtcg cgttcgacga agaccaggag ctttcgcgca
    43201 agctctccgt ccgagggctt ccgacgacac tggtgatcgg gcaggatggc cgcatcgtgg
    43261 gccgcgccgt tggccctcgg gtgtgggaca gttccgaatc aattgccttc gtgcgacacg
    43321 tgctggccac agacacgcca tgaagcgctg tctttctgct ctcgcgatcg tcctggtcgt
    43381 gctgtatatg gctctcgcaa ttggcgccgc caattgtctc gtccttcacg cggacgtgcc
    43441 gattccccat caccatagcc actcacatgt cgcgcattcc ctcctctgtg cctgggcgtg
    43501 ccaagccagc ccgagcgtgg cggttgccgc cgctgtgccg gttgttgtca tctcggttct
    43561 tgtggctatg gtgcgatcag ccgattcaat ccaccgcgcc accttgtttg actccgagtc
    43621 cctctctcgc gcgccccctc gttgacctgt ctcgcgggta tttctcactc aacatcgtct
    43681 gtgctgcgaa cggaagagct ccatcatgcc gttcgagggt tcgtgcgttt gtcatctgct
    43741 ggaagggagt tggcgatcat gtgggcacag cggcatggat ctgtgaagcg attatccatc
    43801 gggttgatcg ggcttgcgtt gttgtggagt tggggggcgg acagagccat ggcgcaagcc
    43861 acacctccgg ctgacacagg aggggcacgc gagcgcgagc tacgtgatca gctcaagaat
    43921 atcttgcagg aattagaaga attgcagcag caaaaggacg gtgctcggcc ggaggaagag
    43981 cgtccttcga tcatcaagga accagccgag tcggcttcgc ctgaagtgtc ggcacggcct
    44041 gacatcgatc tggccgatat gagcattgtc agccaacggc tccagaagcg gcctgaaggg
    44101 atctccttat ctgcgaccgt tccggcagaa acagattcgc aaccaacccg gacgatgaag
    44161 gagtccttgg aatctctgcc gggagtggtg ttgcgtcaag ccaacggacc gcgggacttc
    44221 agcatcatga tccgggggca gggcgccaaa accacattcg cggttcgcga tatcaaaatc
    44281 tatgaagatg gattcatcca gacgcaatcc gacgggctgt cccggctcga catccacgat
    44341 ccctggttca tgcgaagcac ggaagtgctg cgcggagcct cgtcttcgct gtacgacaac
    44401 tatgccctcg gcggcatggt ccacttcaga accaggcgcg gcagcgacat caacgggttt
    44461 gaaaccttcc tctccggagg ttcctacggc ttcttcaagc aggcctttgc cgtcggccag
    44521 caatttgaaa accttgacgt gtcgttattt gggagtaacg ccgcagaaga cgggttcatt
    44581 cgaaacagcg attacaacac ccagacgctc aacttcaatc tgcgtttcac catcgacgat
    44641 aagcaaagtt tttatttcaa agcaatcacg aactggctgg atacgcgcgt gccgaccaga
    44701 ttgactcaat cacagttcct tactgacgag cgtcaggccg ggggcgccca gacggcgtgc
    44761 acgccgggcg tctacaatcc caactgcgcc aatgcctcgt tgctccatca gcagcgcatc
    44821 gatcgccgca cgattgttgg cggcatctat gagcgacaac tcaacgccaa taccgtgctc
    44881 accattgaag ccgactacga cgtgaaagat atcaatcaat acttctcaca gattacagac
    44941 aatgtgaatc ccaactataa gaactatgtg gacgtgcggc atgacggtcg gctggcggag
    45001 atgcccctgc gaagctatgt cggcttcttc gtgaacaata tggagcagga agggcaaacc
    45061 tttcaaaatc ttgcggatgg caacggcact cgcggcctgc tgattcaaaa caaccgaggc
    45121 acgatccgca atatcggcgg acgtttcaga gaggaactcg aatttgttcc gaaatggatc
    45181 ctcgcggggg ggctcgggtt cgagcaatcg atgatcagta tccagtccat caactatgag
    45241 ggagccacgc cgttgcgagt caacgcggat cgcacgtact acaactgggc gcccgagatg
    45301 tccttgacct ggaagccggc tgagggttat cggcattgga tccgggcttc cacagggtac
    45361 ggcattccgc aattcaataa cttactgaga gatccattca cgggccaacc tggaaccaat
    45421 tttgacctca agccgcaaaa aaatctgaat acggaaatcg gcacagagtc gaggctgacg
    45481 aaggatttgg tcgtggaact ggttgggttt tggaccttct tcaagaatga aatcattact
    45541 cagaccattt cagggaacaa cgttgcctcg gtgaatgccg actcctcgca atatcgcggg
    45601 atcgaggctc gatatgactg gcgccccctt acgggattgc gtttctcagg ggcctacacg
    45661 catctcgatg cgagatatgt caatttctcg gatcgcaccg cggcaggctt cttggttcgc
    45721 gatgggaaca aggtgcccaa cgtgccgact gatatcttca acaccaaagt tgagtacgat
    45781 cacgcgccta ccggttgggg aggttggatt gagggcagct attacaacag ctatttcttg
    45841 aacaacagca acaccttcgg catcccgtcg tatatcgtca tgaacgtgaa cgtccacaag
    45901 agcatcgaaa tcaaaaactc ctggatacga ttcgcgaagt tctatctgga actggataat
    45961 attgcagaca agaagtatgc ggcgtccggt caggtgatcg gcggggaaac gactggtgca
    46021 gcagccggcc agcaggcctt cttcgccgga tacggtcgag ccgtctatgg aggggtgacg
    46081 ttgggccttt tctaggctcg agtttttgct gaggcgctac tcacacacgc ggcccttcgc
    46141 gtttgatttc gataccggcc ccccgtatag ttgaaacaca cctcgcatag cacggagcgc
    46201 tctcttgacg gtacgtgcag ctcgactctt ttgggcgatt ctgagcgttg tgtcttgcct
    46261 tttctttcct ggcggtggat ctgctgccgc acctgccgat tcggacaggc gcgccgtcga
    46321 ggaagcgacc gaactcttca gagccggcga gatcgatgcc gctattgtcg ccttgcgcaa
    46381 ggcgacagag tcggggtctt cttcagcgga agcctaccat ctactgggcc agatgtattt
    46441 caaaggcaaa aaaaagcctc aggacgcggt cgaggcgtat acgcatgcgc tcaagctgaa
    46501 gccggcgtat cccgatgccc tcaatgatct ggcggaagta tatgtcgcac agggcaagac
    46561 ggcggaggct gaacagacgc tgaaacgggc aattgaagtg gcgcccaagc gcgaagagtc
    46621 ctatgtgaac cttgcgcggc tgtatgagag ccggcacgac gttcccgccg cgatcaacac
    46681 ctatcagcgt ttgttgacag tccgtccgac gagtgcggac ggactgtttg ggttggcgat
    46741 tctgcacgaa gggcaaggca acaacaagtc cgcccgtgaa ttgctcgcac gtttgacgaa
    46801 agccaatcct gctcatgccg acggttggta tcaagccggc cgattggccg aacgaagcaa
    46861 tgacttactc gaggctgcct atgcgtaccg gcaggccatt gccgccaaac cgaacctggt
    46921 cgatgcccac tataaccttg ggttcattct caggagtcaa aacaagccgg cggaagcaga
    46981 aaaggaattt ctagaggtta tccgctatcg ccccgagtat gccgaggccc atatgaacct
    47041 tggggtggtc tatacgagca tgaataagct ggaagaggcg gaacgggaat atgagagagc
    47101 cgtggccttg aagccagatt atgccgaagc ccactacaat cttggcgtgt tctatgaatt
    47161 acatcgaaag gacatgccgc gggcgctcgc gcaatatcac aaatacagaa atcttggcgg
    47221 ccgagacgac cgggtggaac ggatcattgg cactgccgga cggtaatgat tcaaagcgcc
    47281 tgagcgacgc gagaacgaag cctggtgagg gcacgtctcg gcgcgcccgg ggggtgggtg
    47341 agaaggcggc cttttcaaca cccctgctta ccagcgatac ggccacggcc gccaatacgg
    47401 actcccatag ggaccataat agtgagaacc catgtagggt cgcatgggga gcgaggactc
    47461 cgggggaggt aagacctcga tgtgacgagt ctcgatcacg ggaaacgtgt actccgtttc
    47521 gtcaagagga agcgtcgtgg atcccgtcac ttcacctgtc acggtcactc gcgtgccggg
    47581 aggaatggtc gcaggatcca aaaagtctcg ctgcatggca atgaagcggc cttgggattt
    47641 catccggtcg gttcccggct ctccggagcg atccagcgga agctgcagga tttctattcg
    47701 ggtgccgtcc tttaatcgtc tggcgctcaa aacttctccg ccgaagagga ccgactgtcc
    47761 tctgtaggaa tccggagcgg ctttgacttg aagaaacgag atttgattcg caggcgatcc
    47821 ttcccccgtg tctgggtttg acgcacaccc ccacaccacc agacagagac ccatcgctga
    47881 gacagcagcc catcgggaac gcatgacgcg aattatacca aacagcaaag cgcagtccgt
    47941 attcgatata atggccggcg ggcgtatgag cctttgagaa aggagggggc atgagaggca
    48001 aaccggctac gtgggcgatg atggtgggaa tgctgatcgc gggtgttcta cttccgacga
    48061 tgcacaccat tcatgcgtca ggaccgcccg agttcaaggg taactatgaa attctgcaag
    48121 atgagcggtc tacgcatcaa cggggtaagg tcaagttggt cgagttcgcg gatttttact
    48181 gcccccactg ccattacttc gatggggaag ggctgccgat tctcgaaaag gaattcggaa
    48241 agaagctcga gaccacgatg gtggggtttc ccgtgatccg aggcaagctt cccacgccct
    48301 tcgatatgta tgaacaggcc aagttgatgg gcaaggggaa tgaaatgaaa aaggtcctgt
    48361 tccggacgat tcacaaggac aaggtgacgg gcgtgctcga ccgctcgata cgcgaagtcc
    48421 tcatcaaaga agtgggactc gatccgaaag tcttcgaaga ggggcttgcc agcggcaaac
    48481 ccgcgcagtt gtttgaggag gggaaaaagt ggggcgagcg gataaagctg cagcagacgc
    48541 ctaccatcct gttggacggc aacatcaagg tcgaaaagat cgatccggag aatctgaagc
    48601 tcctgattca tagtatcctc gatgcggatt ccaaaaagtg acgcccgagc ggtgccggag
    48661 ggtggtgaag ctatccaccc gtccgcagcg aaggaaaagg ccaggcagct tgagtggcca
    48721 gatgaagaac gcaatgcgga aacggcgatt cgcgaggcgc cggggtaacg ggtgagtgtt
    48781 tacggagtgg ttttcacatg gctgtagacc ctgtttgcgg gatgactgtc gatccggcca
    48841 ccgcagccgg acagtatgac tatcaaggtg tgacctacta tttttgcgcg acgtcctgcc
    48901 tcgagacctt caaacgcaat cctcaactcg cactgcaggc tcgagagccg gagttcatta
    48961 cgttggggag gaagaagcca ctgacgatga tgcctgcatc gacctcggtg gaatcgggga
    49021 acatcgatcc ggtttgcgga atgaccgttc aaccggaaag cgccgccggc tcccatgtcc
    49081 atgagggcaa gcgctactat ttctgttcac ccggctgctt gaccaggttt cgtgagaacc
    49141 ccatccatta cctgatccct ccagagcagc ggctacagaa acccatagcg gtaccggccg
    49201 gtgcgaccgt ggaatacatc tgtccgatgg atcccgaaat cctcgagacc aagccggggg
    49261 tctgccgcat ctgcggcatg gcgttggaac cgaaggttgt cacgctggac gagcgcccaa
    49321 atcccgaact tgtggacatg agcagacggt tgtggatgag cgtaggtccg gcgctgctcg
    49381 tgatgctgtt cgcgatggcc gacatgattc ccatgcttcg cgatatggtg gatcattcag
    49441 cgagaaactg gattcagttg ctgctcgcca ctccggtcgt tctctgggga ggccggcctt
    49501 tttttgaacg ggcctggatg tcactcgttc accgggcgcc caacatgttc acgctgatcg
    49561 gactgggaac gggcgccgcc tatctgtaca gcgcggtcgt gacgattgca ccgggcatat
    49621 tgccggcatc cttccgcgcg cacgatggat cattggccgt ctattttgaa gctgcagcga
    49681 tgatcacggt gctcgtcctg ttagggcagg tcctggaatt gcgcgcgcgc agccaaacga
    49741 cctcggcgat gaaagccttg ctccgtttgg ccccgaaaag cgccaggagg atcgacggcg
    49801 ggcgagagac cgatgtgccg ctcgaacacg tccgggtgaa agatctgttg cgtgtgaggc
    49861 cgggtgagcg ggtgccggtg gacggagtcg tcatcgaagg caccacggcg gtcgatgaat
    49921 cgatggtaac gggagaacct ttgccggtgg caaagggccc tggcgcgtca gtgaccggtg
    49981 gtaccattaa tggaacaggc agcatcctga tgcgggctga gcgggtcggc agcgaaaccc
    50041 tgctggcgcg catcgttcag atggtgagtg aagctcaacg cagccgcgcg ccgattcagc
    50101 gagtggccga cagtgtcgca ggatattttg ttccggtcgt cgtcgccata tccatcctga
    50161 cggcagtggg gtgggcagtg tggggccctg aaccgaagtt ggcgtttgcg ttggtgaacg
    50221 ccgtcgcagt cttgatcatt gcgtgccctt gtgccctggg cctggcgaca ccgatgtcga
    50281 ttatggtcgg gacgggacgc ggggcaacgg ccggagtctt ggtccggaag gcagaaacgc
    50341 tcgaggtgct ggggaaagtg gatacgctcg tttttgacaa aaccggaacg ctcaccgaag
    50401 gcaagcctaa attagttgcc atccgcttcg tacctccctg gaacgatgct gaggttctgc
    50461 gcctggccgc cgcgctcgag cgccggagcg aacatccgct ggcgtcagcg atcattgccg
    50521 gcgccgacga acggcagctt acaccgggcc tggtcgagca atttcagtcg atcacagggt
    50581 acggggtgat gggggtggtt gaccagcacc gggtggccgt cggtaccacg caatttctaa
    50641 gggaatatct caagatttcg gatcccggcc tcgcggtcct ggaagaagag gccgcacgct
    50701 taagagctga gggtcaaacg accgtatttg tcgccgtcga cgataaggcc gccgggttgt
    50761 tgggcgtcgc cgatccgatg aagccatcga ccgcggaagc ggtccgagtc ttgaaaaaac
    50821 agggcatacg cttggtgatg gtcacgggtg atcaccaact cacggcagat gccgtcgcgc
    50881 ggcggctggg aatttcagaa gtccatgccg gtgtattgcc ggatcacaaa ggtcgcatta
    50941 tcgcaacact caaatcgcag ggccgcgtgg tggcgatggc cggcgatggt atcaacgatg
    51001 cccccgccct cgcgctcgct gatgtgggca ttgcgatggg aaccgggacg gatatcgcga
    51061 tggagagtgc agggatgaca ctcgtcaagg gtgatctccg ggcacttgtc cgcgccgtcc
    51121 gcttgagccg agccactatg gccaacatcc ggcaaaacct cttttttgca ttcgtctata
    51181 acatggtcgg tgtgcccatt gcggcgggag tgctgtatcc gttgtggggc ctgctgctca
    51241 atcctatgat tgcgagtgct gcgatgacct tcagttccgt ttccgtgatt gccaacgcgc
    51301 tccgcctgcg tcatgtggat ttatgagtgc gtcctctccg tctccatgct agggtacagc
    51361 gctttgaacc gctcacataa tgtgtccatg agaaaggctg gtatggcggc ttcggtttct
    51421 ttccttgtgc gggtattcgc tgccgccgtc ctcacactgg gcacgtcgac ttggggagag
    51481 gccgcgggtc gcctgtttcc ggtcactagc tcgaccgatc tccagaatca ggtcaaggct
    51541 actgcatcca aagtcattcc tgccgtcgtg agcatcgcct ccacagtcat ggtgcgggat
    51601 caggccttca gcgatgaaac gcttcccttc ggattgttca gagaacctcc ggcccggcga
    51661 cagtatgggc agggttcggg ggtcatcgtc tcttccgatg gctacatcat caccaacaat
    51721 cacgtggtgg cggatgccgt cgatgtggaa gtcattctag ccgaccggcg acaattcaaa
    51781 ggaaaggtcg ttgcgactga ccccaagacc gacgtcgccg tcgtcaaaat ccaaaccact
    51841 aatttgccta cggttccgtg gggcgattcc agcaatttgg cggttggcga cttcgtattg
    51901 gcaatcggca atccgttggg gttgagccgc acagtgacat ttggcattgt cagcgcagtc
    51961 gggcgcgcgg atgtgggggt ggcggatttc gaggacttca ttcaaacgga tgcgccgatc
    52021 aatccgggaa attccggcgg cgcactcgtc aacacgaacg gggagctggt gggcatcaat
    52081 acggctatcg ccagtcccac gggagggagc gttggggttg gctttgccat cccgagtaat
    52141 atggctcgag ccgccatgca gagtctgatc aaaaccggac gcgtggtacg aggatttttg
    52201 ggcgcgtcca cacaggacgt cactccgctg ttgggaaaga tctttcggct gcctgatgtg
    52261 aaaggggcaa ttgtcacgga tttacaggcg aagggatccg cggagaaggc cggactgaag
    52321 cgcggtgacg tcattgagcg gtttgacgga cgggacgtca tggacagcgg gcatctccgc
    52381 aatttgatcg ccgcagcggc catcggcagc aagcaccgcc tcgatctcgt ccgggaaggc
    52441 aaaatgatac aagtcgatct caccgtgcag gaagcgcctc gcgagcgagc aaaaaaggcg
    52501 caagcggcgg ccgcaaccac ctcaggcgcc catcccttat ccggcgtcgt attcgacgat
    52561 gtgactcctc ccttggccag gcaaatggac ttaccggtca gcagcggggt ggtcgtgacc
    52621 gatatcgaag aaggcagttc cgcggaatcc tctggcctgc agcctggcga cgtcatcttg
    52681 gaattaaatc gtcagccgat tccgaatttt tccacgtttc agcgcctcgc cgaccccatg
    52741 aaatccagtg atctcgcact gctcctcgtc aaccgtcaag ggaccattct ctacatcccc
    52801 gtacgggggg aatagcacga cctcctccgc gactccaaaa ttgactcgct gtgctcctgc
    52861 ggctatactc cgtagcacga atctgataat cgtagcatca cataatgaag aagctcgttc
    52921 gattcggcgt ctctttggat caccacctcc tcgctgagtt cgacgacgtc gtccgtcgcc
    52981 gcaaatatcc cacgcggtcg gaagcgcttc gcgatctgat tcgcgatcag ttggtgagtc
    53041 aggaatggag cggcaatccc ggacgtgagg tggtggcgac gatcaccttc gtttacgacc
    53101 atcatgtacg cgatctcacc aggaagctca cgcatatcca gcacgagttt caaggacatg
    53161 tcatggcggg catgcacgtt catctcgatc atgatcactg tcttgaagtg ttggtcgtga
    53221 aaggcaaggt gtcggagatc aaggaagttg ccgatgcgct agtcagtgtg aagggcgtga
    53281 aacacggcaa gctgaccatg accacgaccg gcaggggact ggtgtgaacg atgcgcctgc
    53341 ggccccggca aaaccagatt caacgacgtc acggtctcat ccacatccgc cacacccttg
    53401 gcgaattgac atcggacatt gcggaagcca atcccgtggc gagggctacg aaggtcctgc
    53461 tgccaatcaa tcgcgctcgg ttggaaatcg ccggcgccga ggatcaaacg cttgacaggt
    53521 tgatcacgcg gtcgctgacg cgggtaacgt cgttggtggt tgcgcccgcc gcaggtgatc
    53581 atatgaacaa aaaccgccag gctacgaggg tttgcccggc ttgatgagtc agccgtcgga
    53641 cgaccgtcgt agacgtctca ttcgccaact catgtattga gatggagggt gaggcgtgaa
    53701 ttatcgctgg ctggtccttc tgagttccgc tgtcgtgttc aacgtaggtc cggtgttcgc
    53761 gcacgatccg gatacggtcg agttggatgt gccggaagtg acggtcctcg ccgatcgctc
    53821 ccacgcttcg tcatctcagc aattcattcc tgataaggaa tatatccttc agccgcaggg
    53881 ccgacctgct caggttctgc ggctgattcc cggatttgtg gctatcgagc actcaggcgg
    53941 cgccggaaag gccgaccaat acttcctgcg cggcttcgac gccgatcatg gcactgatgt
    54001 cgcatttttt tcagacggca tgccgatcaa ctttcgctct catgcgcacg gacagggcta
    54061 taccgacctg aacttcatca tcccagagac gatcgaaggg cttgatgtgt cgaaaggcgc
    54121 gtatcttccg gagtacggcg atttcgatac cgccggagcg gtcaactttc gaacccgcga
    54181 cgtcgtaaaa gagggaatcg tacaggcggc aggaggacag ttcgacacgc agcggtatat
    54241 tctcatgttc tctccgacga aggacaggat ccgcaccttg ttcgccggag agggctacta
    54301 cacgaacggc ccttttctca acgacaaccg gtattttcgc gcgaacctgc ttgggaaggt
    54361 caccacgaac ttgtccagcc gcgatgaatt gagcttgacg gcaacatttc acaaagcgca
    54421 atggaacgca tccggagaaa ttccgctgcg cacggtcaca gacggcaccc tcgatcgctt
    54481 cggcgccatc gatccatcgg aaggcggcaa gaccctgcgg agtacggcca ggttgaacta
    54541 tcactacgac actcagtcag gaggtgaatt gttcgcgaat gcctatggcc agtactataa
    54601 gtatgacctg tacacgaact tcaccttttt tttgaacgat cccgttaatg gcgacggctt
    54661 tcagcaacac gatactcgag tgatgtatgg cggcgacctc gggtacaaac atcgggcaca
    54721 gttttacggg atgcccagta cgggcacgat cggtttccaa accagggtcg atgacatcca
    54781 tgcacggctg ggcacccagg tgagacgcaa tccgcttagc acgacggccg atagcaacat
    54841 cctggaggcc tcttacgcac cctacctcaa agccgaggtt caacccttgc cctggatgcg
    54901 attgacggga ggactccgcg cggagacgtt caccttcaat gtgcagaacc gttgcccgga
    54961 ttgtgcagag cagccggcgg gacgcacctc ctcgagcatc gtgctcccca aggccaatct
    55021 gattctgggc ccatggttcc agaccgagtt cttcgccaac tacggcgaag gctaccacag
    55081 caacgatgca cgttcggcgg tcactccggc ttcttcaccg ctcgctcggg cacgaactta
    55141 cgagttgggg gcacgttcga aaccctgggg acccgagggg atggaattga tcgcgaccct
    55201 ctgggccatc gacctcagat cggaattggt attcgtcggc gatgaaggga cgactgaaat
    55261 ccgcggcccc actcgccgtc gaggcgtgga ggtcgcagcc aggggccaga tatggggacc
    55321 gatctatatc aacggcagtc tgacgtggtc gaaatctgaa ttcgtcaacg gggacgcgat
    55381 tccgctggcg ccggaactca ccgcctatgg cgccgttctc gttcaatggc cggaaggctt
    55441 gcgctcacaa atccaagcga cgtatctcgg cgttcgtccg ttaatcgaag atcggagcgc
    55501 caacgccccg tcctggatcg acattgatct atctgaacga tatattttgc cggtcaagct
    55561 gccatatggg cgactggagg cgttcctctt cattcagaat ctcctcaaca ccaaatggga
    55621 acaggccacg ttttattttg catcacggtt acgaaacgaa gcagccggcg tcaacgacgt
    55681 tcactttgtg cccggcaatc cccgattctt tatgggcggc ctggcctggt acttttagca
    55741 ggccgctgag aatgaccgct cgcttcgttc tcggtggcgg cccgctctcg acgtagcaaa
    55801 cagcgtacgc ctcgagcgtc ccgctccctg cagtcttgct agatgtcatt ttttgagtag
    55861 cctgcaatta gaatagcgcg tcgagatcgc acacgtgaaa cgccggcata gaattctctc
    55921 taaatcctat ttcaaccgtt cgactttagt ggtcgccaag tcgctgttgg gtaaatatct
    55981 ggtgcgacaa aacgggaagg agacaattgc gggcaagatt gtagaggtcg aggcatacgt
    56041 cgggccgcat gatttggcat gtcacgcatc gaagggccga accctgagaa ctgatgtcct
    56101 tttcggacca ccgggaatct catatgtgta cttaatctac ggaatgtacc actgcttgaa
    56161 tgtcgtgacg gaacgagcag aatttcctgc cgccgtattg atcagggcac tggagatcga
    56221 tggagcactg gttgatggtc ccggtcgact ttgccgcgcg ctggacatcg atttatctct
    56281 gaaccggcta gatatgacgg ccggacggca tctctggatc gaagatcgag ggctgagggt
    56341 tccgcaggct caagtgggca cgttcccacg aatcggcgtt gcctatgcag gaagctgggc
    56401 agagaaaccg tatcgtttca gaatcatctg agaaggctgg aggacgaaca acaagggagt
    56461 gacccgccgg gccactccct tgtcagctag cagaagcgat tagtcgatct tgcgatcgcc
    56521 cgtaatcttc gttgcagagt tgacaggtgg agcaatctgg atctgcggac cctggacttt
    56581 cggaaggcgc ccggcaccct cgctcgccgt gatccggtcg ttgtccactt tcttcagatt
    56641 ttgcgacaca tggttgtcgt cgaagcccgc ggctttcgtc aacgttgatg cgcctcgggc
    56701 atcagactga ccaggatcgt tcgcggtcgc ctgtccgttc actgggctgc cgctgttctt
    56761 catcggatag ccctcatgct tgggcagcag cgctgggttg gccgaggcca tggagaccgc
    56821 aaacaagaca ccggaaacca tcgcaacagt gccgaataag agcttcatac ccttctcctt
    56881 tgaataatgg tggccgaaga atgccgtagt tgcgctccga tatcaacagc gaatcaagtg
    56941 ggcgcgatga tagccagagg gaacgaggcc tgtcaagcac aaagaggccg gttccggctg
    57001 ggagagaacg gcgagagggc acacgagaaa gtttctgctc gcggcgcgca ggatctatcg
    57061 tattgttgca taaggcagtg cgcgcttgga tggtggccca tctgtttcca gcgggtgagt
    57121 ggggcgagct gagatattgg cacctcggga cgtgccagtg gagggaaaaa ggctgtgggc
    57181 agttaaacga gtcccgaaac cggccgggaa ggaacacaat ccccgatagg gatggactat
    57241 cgccgatggg ggcgacggct gcgaccccga tgacggaagg acggaccgcg gctcacaccc
    57301 ggcagcctga tagtgaccgt cgtgccctcg cccggtgcgc tgccgatggc aatggagccg
    57361 gcatgttctt ccacgatctt cttcacggtc gcgagaccca agcctgatcc ggcttttttg
    57421 gtcgtaaaaa acggctcaaa gactttatcg acgatttccg gcggaatcgg tgctccgtcg
    57481 ttcttaatga gaatatgatc ttcgatgcct cgtcccgccc gatgttgtgc cacttggatc
    57541 gtcagtcgtc cacctgggct catcgcttca caagcattct tgaaaatgct gagaaacacc
    57601 tgctgtagct tggcttcgtc gcctcggaca ggcgcaagga cgggagcata ctctttctcg
    57661 acttggattc tcgtgatttc gaggtcattg gccaccacgg tcagcgcgct ttcgatgacc
    57721 tccgggatac ggacgaggcg tcgattcaat tccagcggct tcgcgaagtc caaaatctca
    57781 ttgacaatcg cttcgaccct catcaggtcg cgcatggcgt tctcgacacg ggtttgggga
    57841 tcgaaatcga gcacgccttc tcccgtgacc tcttccaact gggctcgaat cgagccgagc
    57901 ggttcccgga cctcagtggc aagaaaggcg ctgaattctc cgatcgcggc ctgacgttct
    57961 tgcttgcgaa taatcggctc ttgtgagatc gcaggcggct gagccggagc agcggaggtc
    58021 ccgccttcgt cgacaacagg cgccggagcg gccgctgccg gcggcggagc ttggagcagg
    58081 tcggcaagtt tcaagatcag cggctccagt gtccgtgcat ccgtcggttg gtagcggtcc
    58141 gcttcccggg aagccaacgt caccgtaccg ccgacctgtc ccctgacaaa aaacggaaag
    58201 accaacgatg actggaatcg atctttgaac agctgcttat gatcgagaaa gcggccttgg
    58261 gtggacgcca aatcgtggtc cacccggggt ttacgatgcc gaatggccca gccggccgcc
    58321 gaactgtcca gagcaaggcg atggcctggc cgcatatctt tcttgccgtc cttgtgctcg
    58381 ccttttaggt ctcctgccat gcccaacact tctacgttgc cggcaatggg atcgtagtgg
    58441 catacccacg atcggtcgta gctgaccgtc tcggcggcgt cggtcaggag ggccgtgatg
    58501 ttctcctgaa gctgcggcgt gagctgcgaa accggcgcgg acaacaatag aggcggggat
    58561 gaagcggcca ccgatacctg ttcctggttc accagcggac gactcaggac cacgttcata
    58621 tcgaagagct tttctcgctc cagggctttt gtgacgtctt cgctggcctg gtccatcaaa
    58681 ccgcgttctt ttttcgtaaa ggccgcgctc tcttttcggc ctatgacgag aaagccgtag
    58741 gtacggtttc gatatcgcag cggcactccc aacagcgact tggctcccgg ggtaatcatg
    58801 cgcaagcgca tcgtgcggcc cccgtcctga tccggtgcgc cagccgtaat gagcgccgga
    58861 gcagaaagtg tgcgtagaat cgcttggacg tcccgagccg tgaatcctcg tgcggcatgc
    58921 ccaacgagcg gaccattctc ccggtgcaaa atagcggcaa gcgctgaatc aaccgggatg
    58981 tcgttgagga ctgagttgag cgctcgttga agctgggtct ctgacggcgg tttactctgt
    59041 gcgatggctg gtgtcatgta tagcgttcag gcaagaaatg cgcgcattgt agcaatgcat
    59101 caacggcaat tcaacgaaaa gcctccgcag agtgctgaaa cacacgtcgt catccgcccg
    59161 cccggcgcgc caggacgcgc tgcggcctcg ctcgacatct ttttgaacgc aaaaaggttt
    59221 ttactctgga gggttgatca cttttggctc cctcggttgc gtccgacggg tgtcgacctg
    59281 gcggtagtcg cccgtgaaaa gattcagcac gctggtgacg acgcttatca cgagcgctcc
    59341 accaactgcc ggccagaagc ctgccaccgt gaaacccttc accatatagg cggtgagctc
    59401 caacagcagc gcgttgagga caaccaaaaa cagtcccagg gagagcacga tgagcggcaa
    59461 ggtcaaaatg aacaacagtg gccgaagcag cagattcagt agtgtgagca ccagcacagc
    59521 ggcaactcct gctccaaagc tgtctgcttc cagccctggc accgtggcca ccgccaaaaa
    59581 caccgcaatg cctgtaatga aaaggcggac catggagctg tggaatcctc ctccgctgaa
    59641 cggagcggcc tgctgctggg aaagatgcac gatacgcact gcactgcctc ctatggagcg
    59701 ggcgtcccag ccgccggcgt ctgttggggc agcggcgctg gctcttgcgc cgttttcttt
    59761 aatgatgaaa aatggacctc ggtggctgtt agggccttgt catccttgat cgcctcgact
    59821 accacacggt caccgacact ggggagattc gcccctttcg gattgccttt ctctttaaac
    59881 cgcgtttgtt tgttgataag cacctcgacg gtcgtgcctt tctgagtttt aacctcgagg
    59941 tgctttgagt cgatcgcgac tacggttccc aatacgtgct ggcccgttcc gtgggccagg
    60001 gccgccgtcg gaagcatcat gcaaagcacc gcgagcacga atgagtgaaa catggtaagt
    60061 ccgctcctct tcctgcgaaa tatgattagt gatgatgccc ttctacgctt ttcccaccgg
    60121 tgtcggtgcc ctcaaggaac cgatcgaacg ccgcttcctc gaggagttcg ttcggagtct
    60181 tcgggttgag cgttttcatt tcgtccagtt cttcctgagt gagctgcggc aaatggcgtg
    60241 taaaatgcac taacttccag ctgtccaagt ccttttccgg atcgccggtg ccccaggccg
    60301 gcatcgccgt aaagcggatg ccattatgga tcacccaaaa gagctcaccg tccgacatcg
    60361 actgcgtctc acgcagtcgt aaatccggcg cctgagggta gacgttcttg ccaatgggcg
    60421 tctgcccgct gccatcattg gcatgacaga ccgcacaatg atccgcaaaa tgggcgcgcc
    60481 cttcccgtag cacctccgcc gtcaagggca cggggttggc aaggttgcgc ttgtcgatcg
    60541 ggatagcgag atggcgaatc tgcctcgcca tccagacctc cagcgcatgc ggctccgtct
    60601 tcgcgctgaa cccggtggta aacagttgat aaccgagcca actcaaaatg cctagaccgg
    60661 ctatgacgat gagaaccgct gcaccgaaca acttagcttt cataccgagt ccgttcacta
    60721 tacaaagctc ttaccctgat tgccacctgc aaaaggttgt ccagtgcatc acgtggcctg
    60781 aagacggctc accgacacca acctttacgc atagccatgg gagcggaacc agcgaaccgc
    60841 cttttccagc gccgcttccg ggggagtctg aggaataccc agttcgcgta tggccttgcc
    60901 acaatcgtag tgcattctgt atttggccat cttgacgcct tccagcggga tgcgaggcgg
    60961 ttgtcccgtc acgttggcca tccattcgtt tacataggca aggggaagaa cggtccaacg
    61021 gggaagccgg agcgaggggg ctttgacgcc cgtgagaccg ctgagaatgt caaaaacttc
    61081 tctcagcatc aggttcttgc atcccaggat ataccgttca ccctgacgtc ctttctccat
    61141 ggcaagcagg tggccggcag caacatcctc aacgtccaca atgttcattc cggtttcaat
    61201 gtaggcgggc atgcggcctt tcatgaaatc gacaatcact tgacctgtcg gtgtgggcct
    61261 gacgtctcct gcgccaaccg gtgcgctggg attcacgatc acgaccggca gcccttcgtg
    61321 ggcgaacttg agcacctctt gttcggccag gtatttcgat cgcttataat gccccgccat
    61381 ttggtccaac gagacagggg tttcttcaga gcccaatccg ccgccgggcg gtaatccaat
    61441 ggccccgatg gtgctgcaat agacggtacg ctcaacccct gcctgccgtg cggctgacaa
    61501 catattacgg gtgccggtga cgttgatgtc gtaaaaaatg gccgggtcct tggcccacag
    61561 cgcatagtgg gcggcgacgt gatacaggtg atgacagccg gtcagcgctg accgaagtga
    61621 gaccgggtcg cgcaaatcgc cctcgacccg ttcgacggaa agacccgaga ggtttttgaa
    61681 atcggagttg gaacggatga ggacccggac attcacaccc gcacggataa gggcacgagc
    61741 gaccgcgcca ccgacgaacc ccgtcgcgcc ggtaacgagg gccttcatgt ggaagagcag
    61801 atggcggata gccggcccgc catggcagag cgtccgcacc ccaagatcgt gccataggcc
    61861 atgcgcgccc ctttccctag cttcggcggc tcgtgatata tcgggcgatc tcgcgcagag
    61921 gatcggcagt agagccgaag gatgagagtc ggtcgatcgc gcgactgacg catgcgtcgg
    61981 ccaactgttt tgctccttcc acaccgtaga atgtcgggta ggttttcttg ccgcgctcgg
    62041 cgtcggtatt gggatttttg ccgagctctt cccgtgtgcc tgtcacgttc agcacatcgt
    62101 cggcgatctg gaaggccagg ccaatgtctt ctgcatatcc cgtcaggtcg tccagttggc
    62161 gatcattggc gccgcctgca atggccccca tgcggacggc ggcgcgaatc agcatgccgg
    62221 tcttgtgttt gtggatgttc tggagggtga gcaaatcaat gtcctggtgt tccgccagaa
    62281 tgtcgaggac ctgcccgcct accatgccca tattgcccga gccgaaggcc agttcttgaa
    62341 tgatccgagc ctggcgtaca ggatcacaac ctttcatgag atcagggcgg ctgatcaagt
    62401 caaaggccat cgtgagcagc gcatcgccgg caaggatggc catggcttcg ccatagactt
    62461 tgtggtttgt cggtttaccg cggcgaaagt catcgttatc catggcgggt aagtcatcat
    62521 ggatcagcga ataggtgtga atcagttcca atgaacaggc cgcggccaac aggccgggcg
    62581 gatttgtacc cagcgcctcc gccgaagcaa tcgtgagtat cggtctgatc cgtttgccgc
    62641 cgcccaacag gctgtagcgc atgctgtcat gaagggtggc cggcggcatg gcggccggtg
    62701 gagtcacttc atccaagaag cggtccactt ccacgcgttt ctgttccaag tagtccttga
    62761 tattcataag agtgcaccgt tgacagaagt gcaaacccgc gcggagagta acaaaggccc
    62821 tagggggagt caaacgagga acctggtgct atcgctgttg cgatgggtaa aacgcgcgta
    62881 tccggagctg ctcggctccg ctgtcccatt catgacatca gccgggcagg gttcttctcc
    62941 ttgtttttgt gcgaatggcc ccgctatact tcagtccatg agcattcgca aagcgggcgg
    63001 tgagtgggaa tcgctgctgt tccctatcca gccgcgacat tgcaaagtct gtcagcaggg
    63061 attgtatcgc gacaaaacca tccttcgtgg tggctggtcc cgctgcactg tctgcgatga
    63121 attcgtgcac tacagttgtc tggcaagtgg gaaattctcg tttctgaagg ctcggcctcg
    63181 agtatgcaaa acctgccgcg cgggccggtc acacccctcg acatccgctg gcgaaacagc
    63241 cgaatctccc gtcgctgttg gctcgtgact cccgagcgta gctcacgtcc acaccccgct
    63301 catcccgctt ggcatcacct gtttgcgcag gtcgttcgct atgggttggc tccggccatt
    63361 ctaagcatca gtggattctt caccgcaaac tttctcgcca gcggtctgta tacctggccc
    63421 agaacgagtc ggatattggc gctctgtctc acgctcctcg ttttatccta cgaattcgtc
    63481 tacaaagaac agtgtgcctg gacggggtcg aaagcagtgg cgcgcgcggc tctgttgtac
    63541 tcctgcgcaa ttccctattt ggcaggtatg gtactcatgt tcgcgctctg gaattggtaa
    63601 gcgttggacg atgggacagg tctttcacag cggggcgttc cttcaacaat gttggtcagt
    63661 gcatccgttg tgcatctcgg tcagacggat caccgcaggc aatcagctca tccttgcttg
    63721 cacatcctgc cggtttgccc atcacctcag gctcggcgat gtcgccgttc ggacttccgc
    63781 atcggaacag acggcgccgt ccaccggaga atccgacgct caattgccgt caaacattcg
    63841 gttcgccgcc tgcctggatg cacatgccgc cgccctcacg ttgcgcgaga tggatgtctt
    63901 cgaagaccgt gccgtgatcc gttgcgcgga atgccgccga ctatatgtct tgacggtgat
    63961 ggcgttcgaa acgcaccaaa aataagatgg cggccgcttc tgcgttgtac gcccaagcat
    64021 ggctggcagc agcaggaggc gctggccaag cttctgacat ggctcaacgg gtcacgatgc
    64081 aaccgctcgt gcccgatgct cttgtgatcc tgctcgacca aatattcgct ctgctcgcgg
    64141 ggtatcagca tgacgaacgt gccgtctttt accgatgagg aaactgcgct gctgatcgac
    64201 gcgcagttct tccagaaaaa ggccacaatc agtgagaaga tccgggccca gttggagaag
    64261 acaggcgaag cattgaaagc agaattgagc cgcggccagc ttccggcgct tcctcatgat
    64321 cacctctccg cgcagttcgt gaggggcgag cacttggaga atttccccta ccaatacctg
    64381 gattgtccca agtacttcca cggtcctgag aagcgtacgt tccgtacgct ggtctggtgg
    64441 ggacaccatg tcgcctgtgc ctggctgctc gaagggcagt tgaccaaaca atacaaagcg
    64501 cgtctcgtcg accgattcca tactcttgca ggacggcatc ttgaattgtc actggcgcca
    64561 accctgtggg agtggaaacg aggtgagggc tacacactgc cgatcactca tgaccggaag
    64621 gctcaaaccg ccgcggtgtt ggccgaacgc gcattcctga agatcgtccg gttcattccc
    64681 tttgacgatg cacggctgcg gagcggggat ttggcaggtc tgagtctgga agcgttccgc
    64741 gccatgctcc cgattgtgtc gctgtaattg tacgagcggg gggatttctg tagactagcg
    64801 cgcattcggt gatcgtttgc ttgcaaccgg atcgccgctg ccggacttgc aacgtcacag
    64861 ggcagcagtt ctgacatgtg gcgtcgaggc tccgcttgcg ccattcttaa ggagggcatc
    64921 gtgatgcatt cgcttatgcg ggtgggcgcc ggaattctgc tgtcggttgg actcatggct
    64981 tcgtcgggct gcggcggaag cggagagacg ctgcatttag aggtgctgcc gccccagcaa
    65041 ccggttcagg tcgcggaacc ggaagccgtc aaaatcgtca ttgaaccgtt tgaagaccgg
    65101 cgggcggata aacaccgcat cgggatgcgc acccacctct ggggtggggt caccaatttt
    65161 aatgtgacag gagaaaagcc gggagaggtg tatgcccagg ccctcgccga tcgcttgagg
    65221 gcatccggat ggctcgatcg gtcatgggat gtgcgtgtgg cacccgccgg ctccgcgcca
    65281 actgccgaca tcgtgatcag cgggcagata ttcgaatttg cggccaatgc gaagagccga
    65341 atgttttcca cctatattac gaccagcaac aaactgacga ttacggcgcg gaacaatgtg
    65401 gatcgtagca gcaccagccg gagcctcgaa ggagcccaga ccgatactgt ggtctggttt
    65461 tcggaagatg acgtcaaaaa gctgatgacc gccaccgtga aagatacgct cgatcgctac
    65521 cttgctgata ccaccatctc acagcgcgcc gtgcgaccga gccggtaatc gtctgaggga
    65581 gatttgatcc gtgcgtgaac gccgctggga gactcgcggt ccgatgacct ttacccaggt
    65641 gttggcggtc ggtgagcgtc tcgctgcgct cgggctcaaa ccggcggtcg cggccaaaga
    65701 cgtcatctgc tacgtggagg aatggagcgt tgaagcaccg gaagacttcg acagcctgga
    65761 tgcctggtcc accgaggacg tgacgttggt gcatgtccgc gacggatggc ggggggactt
    65821 ttttctcctc gccggtgcct accacaccac ctatcttcac tatcagaacg tcgggaccta
    65881 ctgttcgatc agtcatcctt ggcgcatccg cgaacgcatg aagctgcacg agaaccgcag
    65941 catgctatgg ctcgggtttc gtcatgccca ttcgtttttg cgtgttcgac tccatacgcg
    66001 agaggtggtc acgcccgggg aaacgcgtga tgacgcgcgc cgagagcaat ggctcgacga
    66061 acgtcgcacc gcttttcttg aagccatcac caccgtcggg cttcccattg aaacggccat
    66121 tgaaaaaggt cagctggtcc ttcgtccgaa cgacccaacg gttccatttt tctgctcatg
    66181 gccggatgcc ttcgggccct gccagttcga atacaacaca tccgacgcgt acgagtttct
    66241 cgttccggcc agccagctgg ccgcgaccta cgcgcctgaa ccggcagggg tgcgcgccta
    66301 tttgaccgga ttttcagacg aggcgctcgc ggaatttgcc cgcattgagc cggtggctca
    66361 tttggcctat cggtgttccg ttcattgtcc gctcgatgac ctgccggaga tccgatctgc
    66421 cattgaacct gacgggcgtc tctatgcgac gctctgcgag ttccagaccc aggagctcat
    66481 tcctgatggc gatgaggcct cggcaattat cggcgtggtc ggatcacgga cgggttttgg
    66541 gatcgaggcg cgcttgaaca aggcgccgct caaagaagat ttgatggccg ggtggctgga
    66601 acgactgatc ggagagccgc tcgtctacgc gccgcttcct gccttcgtct gacgagactg
    66661 ctgaaacaac gtcagcttcg tgctcaggtc ttctcctctg atcctattcc aagtcacatt
    66721 ttcgttgctc aaagtcatcg gattccgcac agtagacaca tgcgcccgct ttccagggtc
    66781 cagtcactgc tggccaagcc gttcatgccc gccgtatttt tcctgtccgg cgtgacctat
    66841 gacaccctca ccttaacgcg gatcgatcgg ctcctcgaca acttggtgct gcttctctat
    66901 ctgttgcttc tgggcctcct gattgtcctc accgggcggc tgggcattga gccaccgccg
    66961 gatccggcgc agctcaagac gctttccccc gtcggacggt gggtgctcca aacccggcct
    67021 ttctatccga tggccagcca gtttctattg ggtgggctat tcagcgcgta tgccatcttc
    67081 tatttccgca gcgccacctt aaccagtacg gcggtctttt ttagcgtgtt ggtgattttt
    67141 ctggtagcca acgagtttct ccgagaccgc ctgtctaacc ttcgcttgct ggtgagctta
    67201 tacgcgctcg tctgcttcgg atttttcacg tttttcctac ccgtgatgac cggctatatg
    67261 aacgtgttgg tctttctcgg cggcgccgtt ttgagcggcg cagtcacgtt tcgcgtcgtg
    67321 gagctgattt atcggaacaa ccctgatcgg tctcggcgcg aagccgtcgg ggtgagcgtg
    67381 cctgcctttg gcgtcattgc cctgttagtg ggattctact ttctgaactg gattccgcct
    67441 gtgccgctct ccatgacgtt cggcgggatt taccatgaag tcaagaaaac agacgatcgg
    67501 tttgaactgt catttgagcg aagttggtat caagtctgga agcaatcgaa cagcacgttt
    67561 ccctctgacg agccggtcta ttgcttcacg gccgtgttcg cgccggtctc cctgaatgcc
    67621 accgtctacc atcattggta tttccgtccg agccgggaac ttccatttag acacgccgat
    67681 cggattcctg tccggatatc cggcggccgg gaaggcggct atcgagcgta taccttcaag
    67741 caacgtctcg atcctgggga ctggcgtgtc gatgtggaaa ccgaggatgg gcgaatcatt
    67801 ggacgagtct ccgtgcgcgt tgagatgagg gccgagccgc agccgccctt aacgaccatt
    67861 gtctattaag aaggaggctg tccttaatca cgggtgacag ggcatcgaac gagtgagcag
    67921 ctcacagaga accggggggt tgccgcaggg tagccaacgg cgtagagtct ctcatagatg
    67981 ccgccacgtc cattgccgcc ttgtccttcg agtcctaatt gtgtgtcgac gcttgcgtcc
    68041 cacgctgaca ctcagcatgc gattgcgccc tttcgctatc agaaatcttt ggcggaggcg
    68101 aaagaacttc tgaaggcact tatcgcgagc ctgccccgta ccagattgat cgaggaggac
    68161 ggggagtacc tccactacga attcaccagc ttgctcatcc ggttcgtcga tgacgtggaa
    68221 tttgttttcg atgaaggcag caagacgatt cacttccggt ccgcctcccg agtcgggtat
    68281 ggcgacctgg gggtgaatcg ccggcggatg gaaaacatcc ggtcgtccct ttcgggacgg
    68341 atttaggaac gaggaaggtg tcccgtcaca atgctctgca ggagacggcc ggggcaagct
    68401 cgtcggccga aaaggtgacc gtcgagagat acgcccaacc catgagagag ccgagctggc
    68461 ggtaggcttc gaattcttcg agtttgtacc actgaaatat cgtgctcgtc tgagggaatt
    68521 cttcgccgat gggggaatcg acgaatttgc tcactttctt gagcgccagc ttcaccttct
    68581 cagatgaggc ccggtccgtc acgagcggaa gcagccaatt cgcatccaaa tgcgtcgggt
    68641 tcaccggcgg actcgtcaag gggtgagtga cgagggatcg accgctctca ggttcgtgac
    68701 tcacgtaaat tttccccgga gcaagaagcg acgggttgtc tctgaacaat atgggattgt
    68761 aggcgtaggg tttgagatag tgaagcacgg tcggaacacc gtccgggcgg atcacccaaa
    68821 atcggcggta ccagacaatc tcattcttcg gcgggatttc accgcaccac tccactcccg
    68881 ggccaaaata gcccttgatg cgcgcttgaa ggttgtacaa gtagtcatat tgccaggtcg
    68941 tatccagggt ggcgtctccc acgatgaggt gcgccacctg gcggcgcaat aaggtctggg
    69001 cgcccaggtt gtcgtaaaac gagccgtcgg tgatttcgat ccatcggctt ttcgtgtccg
    69061 gccagatgcg ttgaatgctc agcatcagca catagtccca aatggtgcca agagtggtgt
    69121 tatggcgacg cgcaaaattc cacgtctgat actcgttgtt caggttgaag ggtgccgtga
    69181 ggatacccag cgtcaaacgg gcgatatcgt cgcgatacca ttcctggacc aggctatcga
    69241 gatcgagccc ggcacctgat gcggtcatgg cttgtgacag acgcacgcag gcgtgcggcc
    69301 tgatccgttc cccccaggtc ttatggcagg ttccctccgt caggtcctgt accacgacct
    69361 ggcggggatt gaatgaggcg gggctgccgt cttgggtttg cacttccacg accggtaagc
    69421 cgaacccggc actctggaca tagcccagtc catcggatcc ggtatagtcg cgcgtgaatt
    69481 caaaattgta gttgtcgcga tagggttggt ccgactcggt ctcataggct cttggacgac
    69541 cgcgattgac aagattccca ttgatgatga ggtaaggcgc atcgacgtcc ttccgattca
    69601 accgaaccag gggggtctcg tgtttccctc gcagataggt cacctcgatt cggtgttgat
    69661 aaggggtgaa gagatgcagc tcattaccga tatcaggcgg cgtcttgata tgtaagacga
    69721 gatcccagac atagtacggg atgagccgcc acagataccc ccaaatcagt ttcggtcctt
    69781 cccaaaaccc cccttctcga ataaagccgg catggtgacg caggtgggtg acaaaatcgc
    69841 catgaggatc gagtagcgtg gcttgatccg gtgtttggac cagattgcca tacgagtcgt
    69901 caagctcctg ccccaagtgg gcgagcatcc agccggcgat gtacgatccg ccagagaccg
    69961 tgctgagata gtcgatctgc ggcaaactcc ccatgtcctg gagcgcctgt aagataccca
    70021 aatgaaaggt tgctgaacgg attccgccgc ctgagaaagc taatccactg agatgcgaag
    70081 gcactgtgtt cgagcgaggg aacgtgtctc cctctttatc gcggtcggag ctcctgacgg
    70141 ctcgccgtcg ttgttcgatg taggcgtatt cctcctgata gtcctggatc agagaggacg
    70201 gggtataggt aaaagctcgt tggcggactt gatacgtttt ggtggcgata ggttcacagg
    70261 acgcccacag caggaggcag aacgccaaat aagctatcgg gtcgatcgtg acacgacgtc
    70321 gtctcaatgg gcagccggcg gcttgggccc ccgtctcgtc gtatgttgga agatgcactt
    70381 tgggcaggtg taatggagga acggtccgct tgcgaccagc cgcttgttca tcggcacctt
    70441 gcaccaggtg cacaggcggt cgggtatttc agacgcggga gacgcaggag cgttagggct
    70501 tgttgacatg gcggcattgt ccccgagcac caagaatctt gtcaaccga
//
LOCUS       DASSCQ010000083        10156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_750210, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000083 DASSCQ010000000
VERSION     DASSCQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750210"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFH05_05870"
     CDS             complement(<1..206)
                     /locus_tag="VFH05_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677772.1"
                     /translation="MSDLTSPRQIMNDLVAHARQLDPYECCGFLAGTDKTVTRLYRIK
                     NVVSLDGASEAASFDEAKMNHLSQL"
     gene            complement(208..1011)
                     /gene="moeB"
                     /locus_tag="VFH05_05875"
     CDS             complement(208..1011)
                     /gene="moeB"
                     /locus_tag="VFH05_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET6677773.1"
                     /translation="MELTERQIERYSRHIILQDVGGKGQLKLSKAKVLLIGAGGLGSP
                     AGLYLAAAGIGTIGLVDGDVVDLSNLQRQILHTTATVGQPKVESGKRTLSAINPEITI
                     NAYHQLVDADNILPLVKQYDVVLDGSDNFTTRFLVNDACFFAKTTLVSASMFRFEGQL
                     TTIKPHAGYPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILAIGE
                     TLADKLLIYDALEMKFRKVSRPKDPDCPLCGPNPRIKDLSTDYTVSCSI"
     gene            complement(1053..1292)
                     /locus_tag="VFH05_05880"
     CDS             complement(1053..1292)
                     /locus_tag="VFH05_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HET6677774.1"
                     /translation="MAHLRFHVRFPEDKVKEPIIYQIGREYNVVTNVRRADVRETTGW
                     VDVELSGETAEVERAIQGLRHKGCVVDPIELNVVE"
     gene            complement(1292..1567)
                     /locus_tag="VFH05_05885"
     CDS             complement(1292..1567)
                     /locus_tag="VFH05_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6677775.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVEVTAGTIVDMIDTLDTAHPGIKAR
                     LCDDSGELRRFVNIYVNEEDIRFLKGKDTSLKAGDEVSIVPAIAGGR"
     gene            complement(1564..2814)
                     /gene="thrC"
                     /locus_tag="VFH05_05890"
     CDS             complement(1564..2814)
                     /gene="thrC"
                     /locus_tag="VFH05_05890"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6677776.1"
                     /translation="MAKMKALVCRECGKEYPTKAIHVCEMCFGPLEVKYNYAEVKQVI
                     SRKKIEEGPRSMWRYADLLPVEGTNYLGPHAGLTPLVRAKNLGAHLGIDELYIKNDTV
                     NHPTLSFKDRVVAVALTRARELGFETVACASTGNLANSVAAHAAAAGMRCYVFIPGDL
                     EAAKVLGNLIYKPNVVEVEGNYDDVNRLCSEIAAEHGWAFVNINVRPYYAEGSKTLAF
                     ETVEQLGWRTPDQVVIPMASGSLLTKIWKGLHEMKTVGLINDVRTKINGAQAEGCSPI
                     ATAFKAGRDFFKPVKPKTIAKSLAIGNPADGYYALKATGESQGSMDMVTDDEVVEGIK
                     LLAQTEGIFAETAGGVTIGVLQKLVKNGTIKKGDVTVAYVTGNGLKTQEAVVDAVGRP
                     VRILPSLVAFERTFRMGKNGGGDA"
     gene            complement(2828..3646)
                     /gene="moeB"
                     /locus_tag="VFH05_05895"
     CDS             complement(2828..3646)
                     /gene="moeB"
                     /locus_tag="VFH05_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET6677777.1"
                     /translation="MEFTEEQISRYSRHILLPEVGGKGQKKIAKSKILLVGAGGLGSP
                     AALYLAAAGVGRIGLIDSDVVDLTNLQRQVLHHTADVGRPKVLSGKEKIQALNPDVSV
                     SMYEERLTAGNALKLFSDYDVIIDGVDNFTAKFLINDACFFADKPLVHGGILRFDGRV
                     TTILPRKSACYRCVFKMPPPPGLVASCQEAGVIGVLAGIIGTIQATEALKLVLGIGRP
                     LTDRLLDFDARKTQFREIKVKRNPQCALCGEHPTITELFDDGDPFAGCAVRPHS"
     gene            complement(3727..4656)
                     /gene="cysK"
                     /locus_tag="VFH05_05900"
     CDS             complement(3727..4656)
                     /gene="cysK"
                     /locus_tag="VFH05_05900"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET6677778.1"
                     /translation="MTMVMHKEITELIGGTPLVRLNRLSPPGSATIYGKVEFFNPGGS
                     VKDRICLNMINEAERQGKLKPGGTIVEPTSGNTGIGLALIAAVRGYKLILVMPESMSM
                     ERASLLSSYGAQLVLTPAWEGMKGSIREAESLLMQNPSYFMPDQFSNPANPAMHKKTT
                     APEIWEALDGKIDAFVAAVGTGGTITGCGEVFKERNPNVKVIAVEPAGSPVLSGGEPG
                     PHKIQGIGAGFIPQVLNRKILDRVIKVTDDEAYQTAKQLSKMEGLLVGISAGANVFAA
                     QQIAQELGPGKNVVTILCDTGERYISIEKYFNI"
     gene            complement(4653..4853)
                     /gene="thiS"
                     /locus_tag="VFH05_05905"
     CDS             complement(4653..4853)
                     /gene="thiS"
                     /locus_tag="VFH05_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET6677779.1"
                     /translation="MQVKINGKSEEFPGGSVLDLLKTKKIEPQMVAVEVNDTMVDREH
                     LDSTPLKEGDRLEFLFYMGGGT"
     gene            5004..5381
                     /locus_tag="VFH05_05910"
     CDS             5004..5381
                     /locus_tag="VFH05_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677780.1"
                     /translation="MWKRNFMFRAADATPLNESENELFHETDPAMDSAGLQLEKFLSV
                     WIQGDGEDEQPVAFTNIYVRTATLDFQKRVGFLQPLQGRTHQIKQLLTPAQKTYLRTW
                     LTSAAPHAWEAADEHFKMLFDLE"
     gene            5378..5944
                     /locus_tag="VFH05_05915"
     CDS             5378..5944
                     /locus_tag="VFH05_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET6677781.1"
                     /translation="MIPPGAHVFVQNRGVRYLLVSTLCLALTLPDCPIIAAGIEVLYA
                     PDDAPLDRVVTLYQQAKRYIYVSVYGLTYPRAVEALVAAKKRGVDVRMLTDQERTQDF
                     KQYRALQTLRLAGIPIRVNQHEGLMHLKQVVIDDEINTSGSMNHTTSGNLYNDERMDI
                     ITDRVLSQKARDRFLSMWNDHARFTEWK"
     gene            5976..7238
                     /locus_tag="VFH05_05920"
     CDS             5976..7238
                     /locus_tag="VFH05_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6677782.1"
                     /translation="MNDVTDVAIVGAGGGGAVLALALAQKSISTIVLDQASGPPSGLR
                     GEILQPNGQQVLDQLGVLRSLPADAMRSVRTFHFCRAGGARLCSIDYGILPPPYNRAI
                     VTLPNVAHHAIVDALHKQATVSLRYDTSFVGLRTERDRVVGLTVQGPQGTDTITAKLV
                     VGADGALSKVREALKIPAELHLYPDGYLIAILESEEPVTEAFYYVGHKEILGLFPATG
                     NMIYLFYMIPSGSYEAIKGRGMGSLHARWTAIAPQFRKLFERLSDWKQTAYMPTGRVR
                     AATWVADGAVLIGDAAHAMNPHASQGRMQAMVDAMTLAELLPSCLADGDCSASRLRPY
                     ETARRPQVTMLQRLADQQVFYWNTGNPLIAYLRDRVFRTLDRNARLRYQVLTTTAGLR
                     TTEPFSIMDRVIATGFLPDLVNMIPRTS"
     gene            7249..7992
                     /locus_tag="VFH05_05925"
     CDS             7249..7992
                     /locus_tag="VFH05_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677783.1"
                     /translation="MGTTERIIEGLPEETQTLLRAYAKDVKAALNDQLEGLLIYGSAV
                     RGEFLTGRSNLNMLLVVNGYDAAVLKRYAPVHKRWSKEGVITPMFLTDHDVRSFSALF
                     PLEFLEIQEHHRVLGGRDPFVGFHVDTRRLQDAVMQGLAGHVFRLRQRYAEAGGADDA
                     VTILLPLAITSTIPLLRGLQRLQGWPVLTQSDAVIKDIADRLQLDLQGFHEALLLKRG
                     QITPGPSEIPRVFDRYLQAATRLVEVMPR"
     gene            7989..8717
                     /locus_tag="VFH05_05930"
     CDS             7989..8717
                     /locus_tag="VFH05_05930"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET6677784.1"
                     /translation="MNQHLPVWIVVMFLLAEAWSWASPYERPTMKLPDPMGYVSDHAK
                     ILEDDWRARIRSVAQDLERKTGVEMVVVTVPTVKPFRSAEDYATAIYEKWGIGSAQQE
                     HGILVLLAVEERQAALTLGRQMLPVITPAVMNQIGTEYLHPAIDRGHFGDGLYRTAVA
                     LASHSQGIRAGTPLRTHYKGMGFWITLFTSIGAIAFFWWISRPDLRHPYRRIQRGEYW
                     GTGQGGFGGNWGGFGGGTNGEGFR"
     gene            8909..>10156
                     /locus_tag="VFH05_05935"
     CDS             8909..>10156
                     /locus_tag="VFH05_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET6677785.1"
                     /translation="MWPPRYSHTWVAFVVWMCASAVLFNTGLGGAAETITLTAPKEPA
                     RADDSFVRLFPGLPPFAPATDEMREKARQLGEKGGILDAQDQLTDPVDSILNPGLNNP
                     DNPTMTAGVTFLGQFIDHDLTLDPRSSLLQPADPRNTKNFRTAAFDLDSLYGNGPQGS
                     AQLYDQSSGDIKFKVEAIPGSEAFSRKGAVRFDLPRDANNNAIIGDSRNDENVIISQL
                     HLAMLRFHNAVVDHLRTKPGISNLSADQIFKMAQRLVRWHYQWIVIHEFLPLTIGQER
                     VDEILTRGPKFYNPHDRRLQNAQGNLLIPIEFSVAAYRFGHSQVRPSYRLNFGPETGT
                     PFFGFAFLDSFDPNDADPGDFRGAKRAPRRFVDWQTFFNFGDGLFRPNKRIDAKLSSV
                     LMELLGMRGAAPGMPSDGVQSLAS"
BASE COUNT         2130 a         2892 c         2840 g         2294 t
ORIGIN      
        1 agctgtgaga gatggttcat cttggcttca tcgaaggaag ccgcctcgga agcgccgtcc
       61 aaactcacca cattcttgat ccgatagagt cgggtcaccg ttttatctgt gcctgccaaa
      121 aatccacagc actcgtatgg atcgagttgg cgcgcgtgag cgacgaggtc gttcataatc
      181 tgtcggggac tggtcagatc ggacacgtta gatcgagcaa ctgactgtgt aatcggtgct
      241 gagatctttg atgcgaggat ttgggccgca gagaggacag tcgggatctt tcgggcgact
      301 gaccttcctg aacttcatct ccagggcgtc gtaaatcaac agcttgtcag caagcgtctc
      361 gccgatggcg agtatttcct tgatcgcttc ggacgcctgt aagattccca ttgtcccggc
      421 aagcacaccg agcacgccgg cttcctggca attgggaaca agacctgccg gcggtggttc
      481 aggatagaga cagcgataac agggatagcc ggcatgaggc ttgatcgtgg ttagttggcc
      541 ttcaaagcgg aacatgctgg ctgaaaccaa tgtggtcttg gcaaagaagc aggcatcatt
      601 gaccaaaaac cgtgtcgtga aattgtcgga cccgtcaagg acgacgtcat attgcttgac
      661 caacgggagg atgttgtcgg catccaccag ttggtgatac gcattaatcg tgatctctgg
      721 attgatggcg ctcaacgtac gtttaccgga ttccaccttt ggctgtccga cggtcgccgt
      781 tgtgtggagg atctgccgtt ggaggttcga gagatcaacg acgtcgccgt cgaccagtcc
      841 aatcgtgccg atgccggccg ctgccaagta caatccagca ggcgatccca gccctccggc
      901 tccgatgagg agcactttgg ccttgctgag tttcaactgg cccttaccgc cgacatcctg
      961 caggatgatg tgacggctgt accgctcgat ctgtcgttca gtgagttcca tgcctcaatt
     1021 caccggttac aggggcgcac gccaggcgtg agttattcga ccacgttcaa ttcgatcgga
     1081 tcaacgacgc agcctttgtg acggagtccc tggatcgccc tctcgacttc ggcggtctct
     1141 ccggagagct cgacatcgac ccatcccgtt gtttctcgca catcggcccg acgcacgttg
     1201 gtcaccacgt tatattcacg tccgatctga taaatgatcg gctctttgac cttgtcttcc
     1261 ggaaagcgaa catgaaagcg gaggtgtgcc attatcgacc tcctgcaatg gcgggaacaa
     1321 tcgacacttc atcgccggct ttgagcgacg tgtcttttcc cttcaaaaag cgaatgtctt
     1381 cctcattgac ataaatattc acaaatcggc gcaattcgcc gctatcgtcg caaaggcggg
     1441 ccttgatgcc ggggtgagcg gtgtccagcg tgtcaatcat gtcgacgatc gtaccggcgg
     1501 tcacttcgac ttcgccctgc cctttggtca acggcctaag cggggtgggg atgcggacct
     1561 tgatcatgcg tcaccaccgc cgttcttgcc catcctgaat gtccgctcaa atgccacgag
     1621 gctgggcaga attcgaaccg gccgcccgac ggcgtcgacg acggcctcct gcgtcttcaa
     1681 tccgttgccc gtcacgtagg cgacggtcac gtcacctttt ttgatcgtcc cgtttttgac
     1741 cagtttttgc agcaccccga tcgtgacgcc gcctgccgtt tccgcaaaaa ttccttccgt
     1801 ttgcgcaagc agcttgatgc cttccacgac ttcatcgtcc gtgaccatgt ccatagatcc
     1861 ctgactttcg cctgtcgctt tcagggcata gtacccgtcg gcggggttgc cgatcgcgag
     1921 tgacttggcg atggtcttag gcttgaccgg cttgaagaag tcgcggcccg ctttgaatgc
     1981 cgtagcgatc ggcgaacagc cttccgcttg ggccccgttg atcttggtcc gaacgtcgtt
     2041 gatcaacccg actgtcttca tctcgtgcag gcctttccag atcttcgtca agagcgaacc
     2101 ggaggccatg ggaatgacga cctgatcagg cgtgcgccag ccgagttgct cgacagtctc
     2161 aaaagcgagt gtcttggatc cttcggcata gtagggccgc acattgatgt tcacaaaggc
     2221 ccagccatgc tcagcggcga tttcgctaca gaggcggttg acgtcatcat aattgccttc
     2281 gacttcgact acgttgggct tatagatcag gttgcccagc accttggctg cttccagatc
     2341 gcctgggatg aatacatagc agcgcatacc ggcagcagcc gcgtgggcgg cgacggagtt
     2401 cgccagattg cctgtggacg cacacgcgac ggtttcaaaa ccgagttcgc gtgcccgcgt
     2461 cagcgcgacc gcgacgactc gatccttgaa tgagagggtg gggtgattga cggtgtcgtt
     2521 cttgatatac agctcgtcaa ttcccaagtg ggcgccgaga tttttggccc ggaccaatgg
     2581 agtgagaccc gcatgcgggc ccagataatt ggtgccttcg accggaagta agtcggcata
     2641 acgccacatg ctgcgcggac cttcctcgat ctttttgcgg gagatcacct gcttcacttc
     2701 tgcgtagtta tacttcactt ccagcgggcc gaagcacatt tcgcagacgt gaatggcttt
     2761 ggtgggatat tccttgccgc attccctaca cacgagcgct ttcatcttgg ccatcgtatc
     2821 accaccttca tgaatgaggt cgcaccgcac agcctgcgaa cgggtcgccg tcgtcgaaga
     2881 gttcggtaat cgtgggatgt tcgccacaga gagcgcattg aggattgcgc ttcactttga
     2941 tttccctgaa ctgcgtttta cgtgcatcga aatccagaag tcggtcggtt aacggacggc
     3001 cgatcccaag taccagcttc aaggcctctg tcgcttgaat cgtgccaatg atacctgcaa
     3061 gcacgccgat gacaccagct tcttggcacg aagccacaag tccgggcggg ggaggcatct
     3121 tgaacacgca gcggtagcag gccgacttgc gtgggagaat cgtagtgacg cgcccatcga
     3181 agcgcagaat gccgccatgc acaagcggtt tatcggcgaa aaaacaggcg tcgttgatca
     3241 ggaacttggc cgtaaagtta tcgacaccgt cgatgatcac gtcgtagtcg ctaaacagct
     3301 tcagcgcatt gccggcggtc agccgttctt cgtacatcga caccgacacg tccggattga
     3361 gcgcttgaat cttctctttc cccgagagga ccttgggccg tccgacgtcg gctgtatggt
     3421 gcaggacttg acgctgaaga ttcgtcagat cgacgacgtc gctgtcgatc aatccgatgc
     3481 gtccgactcc cgcggcggcc aggtagagcg ccgcgggcga acccagtccg ccggcgccga
     3541 ccaagagaat tttggacttg gcgattttct tctggccctt tccaccgacc tcgggcaaga
     3601 gaatatgccg actatagcgg cttatttgct cttcggtaaa ttccatgttt caactcgatg
     3661 ccgaaatggt gtccagcttc ctagatgcgg ctttgccccg tgaagcgcgt gggatttcct
     3721 tctcgttcaa atattaaagt atttctcgat gctgatgtac cgctcgccgg tatcgcaaag
     3781 aatcgtcacg acgttcttcc cggggcccaa ctcttgggcg atttgttgag cggcaaacac
     3841 gttggcgccc gccgagatcc cgacgagcaa tccttccatc ttcgacaact gcttagccgt
     3901 ttggtacgct tcgtcgtccg tcaccttgat gacgcgatcg agaattttcc gattcaagac
     3961 ctggggaatg aatccagccc cgatcccttg gattttgtgg ggcccgggtt caccgccgga
     4021 cagtaccgga gaccctgccg gttcaactgc gatgactttg acattcgggt ttcgttcctt
     4081 gaagacctcg ccacaccccg tgattgtccc gccggtgccg acggcagcaa cgaaggcgtc
     4141 aattttccca tccagcgctt cccaaatctc cggagcagtg gtctttttgt gcatggcggg
     4201 attagccgga ttcgaaaatt gatcgggcat gaaatacgac ggattctgca tcaagaggct
     4261 ttcggcttcc cggatcgacc ctttcatccc ctcccaagcc ggcgtcaaca cgagttgggc
     4321 accgtatgac gaaagcagac tcgcccgttc catgctcatg ctttccggca tcacgaggat
     4381 cagcttgtaa cccctgacgg cagcaatcaa ggccaacccg atgcccgtgt ttccactcgt
     4441 cggctcaacg atagtgccgc cgggtttcaa ctttccttga cgctccgcct cgttgatcat
     4501 gttcaggcag atgcggtcct tgacgctgcc gccaggattg aagaactcca ccttcccgta
     4561 aatcgtggcg cttcccggag gggaaaggcg gttgaggcgg acgaggggag tcccgccgat
     4621 cagctctgta atctccttgt gcatcaccat ggtcatgtgc cgccacccat atagaacaga
     4681 aattcaagcc ggtcgccttc cttgagggga gtcgagtcca ggtgttcgcg gtcgaccatt
     4741 gtgtcattga cttcaaccgc caccatctgc ggctcgatct ttttggtctt cagcaaatcg
     4801 aggacactgc ctccgggaaa ctcctcagac ttgccgttga tttttacctg catggatctc
     4861 ctaacttggc ccaggaatta aggaattcgg acagagtaac aaagtggctt tcggcttgtc
     4921 aaggcgacga ccgctcgcga gcgttggctt gactttgcgc ttggaccgcc gccactatgc
     4981 cggttccctg gagggatgtt ggcatgtgga aacgcaactt tatgtttcgc gccgcggatg
     5041 cgacgccgct gaacgagtcc gaaaacgagc tgtttcacga aaccgatcca gccatggaca
     5101 gcgccggcct acagttagag aagtttttgt ccgtttggat tcaaggggac ggcgaggacg
     5161 agcagccagt cgcctttacg aacatctatg tgcggactgc caccttggat ttccagaagc
     5221 gcgtcggatt tcttcaacct ttacaggggc gtacgcacca aatcaagcaa ctgctcacgc
     5281 cggctcaaaa aacctatctg cgcacatggt taacgtctgc tgcgcctcat gcctgggaag
     5341 ctgcggacga acattttaag atgctgtttg acttggaatg atccctcccg gcgcgcatgt
     5401 cttcgtccag aatcgaggcg tccgctacct cctcgtatcc acgctatgtc tggcactgac
     5461 acttcccgat tgccccatca tcgctgccgg catcgaagtg ctgtatgcgc cggacgatgc
     5521 cccgctggac cgtgtagtga cactctatca gcaggccaaa cgatatatct atgtatccgt
     5581 gtatggcttg acgtatccgc gtgccgtgga agccttggtt gcggcgaaga agcgcggcgt
     5641 cgatgtccga atgttaaccg accaggagcg tacacaggac ttcaagcaat accgtgcgct
     5701 acagacgttg cgactggctg gcattcccat ccgtgtcaat cagcacgaag gcctgatgca
     5761 tctgaaacaa gtggtcatcg acgacgaaat caatacgtcg ggctccatga accacacaac
     5821 cagcgggaat ctgtacaatg atgagaggat ggatatcatt accgatcgcg tcctctcaca
     5881 aaaagcccgg gaccgcttct tgtccatgtg gaacgaccac gccagattta ccgaatggaa
     5941 ataacgcccg acatgcagga tgccggtgcc aagcgatgaa tgacgtgact gatgtggcta
     6001 tcgtcggagc cggaggcggt ggggccgtat tggcgctcgc gttggctcaa aaaagtatct
     6061 cgaccattgt cctggaccaa gcgtccgggc cgcccagcgg cttgcgtggg gaaattctcc
     6121 agccgaatgg acagcaggtc ctcgatcagc tgggggtgct acgatcactg ccagcggatg
     6181 caatgcggtc ggtcagaaca tttcactttt gtcgcgccgg cggcgcaagg ttgtgttcga
     6241 tcgattatgg tattctcccc cctccctaca atcgggccat tgtgacctta ccgaacgtcg
     6301 cccaccacgc gatcgtggat gcgttacaca aacaagccac ggtcagtctc cgctatgaca
     6361 cgtccttcgt gggccttcgg acagagcgtg accgagtggt tgggcttacc gtacaaggac
     6421 cacagggaac cgacacgatc acggccaagc tggtcgtggg ggctgacggg gcgttgtcaa
     6481 aggtccgcga ggcgctcaag attccggccg aattacatct gtatccggat gggtatttga
     6541 tcgccattct cgaatcagag gagccggtaa cggaagcgtt ttactacgtc ggccataaag
     6601 aaattctcgg cttgtttcct gcgaccggca acatgatcta cctgttctat atgattccca
     6661 gcgggtcgta tgaagcgatc aagggtcgcg gtatgggatc gttacacgct cgctggaccg
     6721 caatcgcacc ccagttccgg aagttgtttg aacgcctgtc cgattggaag caaacagcct
     6781 acatgccgac gggcagggtt cgcgccgcca cttgggttgc tgatggagcg gtgttgatcg
     6841 gcgatgcggc acatgccatg aatccgcacg cctctcaggg aagaatgcag gccatggtcg
     6901 atgcgatgac gctcgccgag ctcttgccct cgtgcctcgc cgatggggac tgttccgcca
     6961 gccgcttacg gccctatgaa acagcgcgga ggccacaggt cacgatgcta cagcgtctcg
     7021 ctgatcagca ggtcttctac tggaataccg gaaacccgct gatcgcctat ttacgggatc
     7081 gagtattccg cacgctggat cgcaatgcgc gactgcggta ccaagtactt acaacaacgg
     7141 ccgggttacg cacgactgag ccgttttcaa tcatggaccg ggttattgcg acgggatttc
     7201 tgccggatct ggtcaacatg attccgcgaa catcatagcc gaggacgcat gggcactacg
     7261 gagcgaatta tcgaaggctt gccggaagag actcaaacgc tcttacgggc ctatgcaaag
     7321 gatgtcaaag cggcgctgaa cgaccagctc gaagggctgt tgatctatgg cagtgccgtg
     7381 cggggagaat ttctcaccgg tcgttcgaat ctcaatatgt tgttggtggt caatggctat
     7441 gatgctgcgg tcttgaaacg ctatgcaccg gtccacaaac gatggagcaa agagggcgtc
     7501 atcacgccga tgtttctcac ggaccacgac gtccggtcgt tttctgcgct cttcccgctc
     7561 gagtttctcg aaatacagga acatcacaga gtgttaggag gccgtgatcc ctttgtggga
     7621 ttccatgtgg atacgaggcg gttacaggat gccgtcatgc aggggcttgc cggccatgtg
     7681 tttcgactac ggcaacggta tgctgaagcc ggcggagcgg acgacgccgt gacgatcttg
     7741 ttgccacttg ccattacctc gaccatcccg ctgttacggg gattacaacg gctacaagga
     7801 tggcccgtgc tcacgcaatc cgacgcagtg atcaaagaca tcgcggaccg gttgcaactc
     7861 gacttgcagg gattccatga agcgctgttg ttgaaacggg gacagattac gcccggtccg
     7921 tcggagatac ctcgggtgtt tgaccgctat ttgcaggccg cgaccaggct cgttgaggtt
     7981 atgcctcgat gaaccagcac ctaccggttt ggatcgtcgt catgttcttg ctggcggagg
     8041 catggagttg ggcgagtccg tacgagcgcc cgaccatgaa gctgcccgac cccatggggt
     8101 atgtcagcga ccatgccaag attctcgaag acgactggcg cgcaagaatc cgttcggtgg
     8161 cccaagacct cgaaagaaaa accggcgtgg aaatggtggt ggtgacggtg cccacggtca
     8221 aaccgtttcg ttccgcagaa gactacgcga ccgccatcta tgagaagtgg gggattggat
     8281 ccgctcagca agaacatggc attttggtcc tgctcgcggt ggaagagcgt caggccgcac
     8341 tgacactcgg cagacaaatg ctgcctgtga tcacaccggc agtcatgaat cagatcggta
     8401 cggagtattt acatcccgcg attgatcgag gccacttcgg cgatgggctg tatcgaacgg
     8461 ccgtcgccct ggcctctcac agtcagggca tccgtgccgg cacgcccttg aggacccact
     8521 ataagggcat gggattctgg atcaccttgt tcaccagcat cggcgccatc gcatttttct
     8581 ggtggatcag ccggcccgat ttacgtcacc cctaccgtcg cattcaacgt ggtgagtatt
     8641 ggggcacggg gcagggaggt ttcggcggaa actggggcgg attcggaggt ggaacgaacg
     8701 gggaaggatt cagatagttt tcgccaatta caagcgctcg catagacaag acgtgtgctt
     8761 ctcacctgtc ctcatatgac tacgactctg caggccccca cgaaagcggt aatcgtaagt
     8821 acgcattgaa aatacatgcc atgctggtat atctgttgct tctccaagaa gggatcgctg
     8881 ggcaatgcct ttggaactcg gagggcatat gtggccacct cgctactcgc acacctgggt
     8941 cgctttcgtc gtatggatgt gcgcgtcagc cgttctgttc aacacggggc ttggtggggc
     9001 cgccgagacc atcaccctga ccgctccaaa ggaacccgcc agagcggatg attcttttgt
     9061 gagactgttt ccgggccttc cgccattcgc cccggcaacc gacgagatgc gggaaaaggc
     9121 gcgccagctc ggcgagaagg gtggtattct tgacgcccag gatcagctga cagatccggt
     9181 tgactccatt ctcaaccctg gtctcaacaa tcccgataat cccacgatga cggccggcgt
     9241 cacctttctg gggcagttca tcgatcacga tcttaccctc gatcccaggt catccctcct
     9301 tcaacctgcc gaccccagaa atacaaaaaa ctttcggaca gcggccttcg atctagacag
     9361 cctgtacgga aacgggcctc aaggatcggc gcagttgtac gatcaaagct caggagacat
     9421 caagttcaag gtggaggcca ttcccggttc tgaagccttt tccagaaagg gcgcagtgcg
     9481 gttcgatctt ccccgtgatg ccaataacaa cgccatcatt ggcgacagcc ggaacgatga
     9541 aaacgtcatc atcagtcaat tgcacctggc tatgctgcgg tttcacaatg cggtggtcga
     9601 tcatctgcgc accaaaccag gaatcagcaa cctttctgcc gaccagatct tcaaaatggc
     9661 gcagcgcctc gttcgctggc attaccaatg gatagtcatt catgaatttc ttccgttgac
     9721 gatcggccag gaaagagtcg acgagatcct gacgcgcgga ccgaaattct acaatcctca
     9781 tgaccgacgt ctgcaaaacg cccaaggcaa tctgctgatc ccgattgagt tctcggtggc
     9841 ggcctaccgc ttcgggcatt cacaggtcag gccgagctat cggttaaatt ttggacccga
     9901 aaccggcacg cccttctttg gctttgcctt tctggactcc ttcgatccaa atgatgccga
     9961 tcctggcgat tttcggggag cgaagcgagc gccccgccgg ttcgtcgatt ggcagacatt
    10021 tttcaatttc ggggacgggc ttttccggcc gaacaagaga atcgatgcca agctgtcctc
    10081 cgtgttgatg gaactgcttg gcatgcgtgg tgctgcaccc ggcatgccgt ccgacggcgt
    10141 ccagtcgttg gcttcg
//
LOCUS       DASSCQ010000084         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_845678, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000084 DASSCQ010000000
VERSION     DASSCQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845678"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFH05_05940"
     CDS             <1..359
                     /locus_tag="VFH05_05940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6677786.1"
                     /translation="EFLQGLHDLHWDVGLAPLVDDSFNRCRSPIKFVEYTACNIATAA
                     SDVGVYRPAMRPGCGLVVQEQEWSSALEQFVTDAQLRRACLDQAREVCARQFSLQEVA
                     AGLLAALEINQSHEAV"
     gene            356..1234
                     /locus_tag="VFH05_05945"
     CDS             356..1234
                     /locus_tag="VFH05_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677787.1"
                     /translation="MKFKNLMKTFSGATEETPRSTGHWTELKPVSMETAELGAEWRQR
                     GHGTAILDLLKDEELQRLNDLLPWSCFVLDAQGRAFGKAWSAKKRAQPQEIPDPRIVE
                     LNRRFPLETRNVFEIGCFEGIHTVALARLSRRVMAADSRIENVVKTIVRCAILGERPF
                     IFPWNVEDGLVRDIDISCDVLHHVGVLYHLVDPVRHLREVLPYVREVVMLDTHVASAA
                     AAVDSYVSGTDRFRYQRYREGGREEPFAGMADHAKWLCEEDLLRVLREAGFHHIDVAE
                     RRQERNGPRILIYASR"
     gene            1364..2413
                     /locus_tag="VFH05_05950"
     CDS             1364..2413
                     /locus_tag="VFH05_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6677788.1"
                     /translation="MLVSHVTGRYIIAAKRTIGGIQYRGFDVSADDRTRLIVRSACAW
                     YPFRLLPVSTGWSIVAMDRRAEGRRFNAVLKERSTDRVLSARSVRLTNTLQPVAFPGP
                     SGVPIDATEADLQLEFCGTGGGRAFLAVHRVLDRSEVIALCRGKGVELGPGTHPQVLP
                     DETREVLYVEQSQSDEWNTLYNEKRRLHVDQESWSKYTIGEAFPLPVEDGSLDFIFSS
                     HVFEHLANPLGHLEHWYAKLRHGGLIVGIVPDVGGSKDYVFEPCSVTGLLEEHASKQM
                     QPQLSHYRKWAAARAPGKDPAEYLKSKRSIHVHFYTAATMQTLLELAIARLGYRTFRI
                     WHTPNHKDFYFALEK"
     gene            2486..3634
                     /locus_tag="VFH05_05955"
     CDS             2486..3634
                     /locus_tag="VFH05_05955"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6677789.1"
                     /translation="MRLVPSVPRLATSKGLVRPSAIGERPAAAQLPDHAHVRGLDELR
                     GLSVLWVMLSHGTGLTTWMPTAFAGYGLHGVVLFFLISGYLITRILVESKDRTDYFSH
                     FYINRLFRIWPLMFLALLVSVLIWPAHAPSAVYNLLLVNNYAYAMGIEPPVRTDVMWS
                     LAIEQQFYLFWPVAVWLFSEKALLLITSLIVLIGLGVDGGLLPQGGVKIVHASTLGSM
                     QYLGMGVLVAFGRNGLKYLLGTWSLFLAWWLTQSAMPVLEFRWIWYGLTFTLALLVYQ
                     TIHAKPLLQSRYLAFTGERCYGLYLIHFFVSALAFIGIGKGVWMAGTVYFILSFLLAV
                     ISFKYFERPIRSLRVHFHQNVRLRVGLFAGVGFMILVNVAYLVIKSIA"
BASE COUNT          741 a          950 c         1090 g          880 t
ORIGIN      
        1 aggagttcct ccaagggctg cacgaccttc attgggatgt cgggttggcg ccgctcgtcg
       61 acgactcctt caatcgatgc cgttctccta tcaagtttgt cgaatacacg gcctgtaaca
      121 ttgcgaccgc ggcgagcgat gtgggcgtct atcggcctgc catgcggcca gggtgcggcc
      181 ttgtggttca agagcaggaa tggagctcgg cattggaaca attcgtgacg gatgcgcagc
      241 tgcggcgggc ttgtcttgac caggctcgtg aagtctgcgc gaggcaattt agcttgcagg
      301 aagtcgccgc tggacttctc gcggcccttg aaatcaatca aagccatgag gctgtatgaa
      361 attcaaaaat ctcatgaaga cattcagtgg cgcgacggaa gagacccctc gatcgacagg
      421 ccactggacg gaactgaagc ccgtttcgat ggagactgcc gagcttggag ccgaatggag
      481 gcaacgagga catggaactg ccatcctcga tttactgaag gacgaagagc tccagcggtt
      541 gaacgatttg ttgccctgga gttgctttgt gctggatgca cagggccgtg catttgggaa
      601 ggcttggtcg gcaaaaaaac gtgcccagcc tcaagaaatt cctgaccctc gcatcgtcga
      661 attgaaccgg cgatttccgc tcgagacacg taatgtgttc gagatcggat gttttgaagg
      721 cattcataca gtggcattag cgaggctctc ccggcgcgtc atggctgcgg acagtcgtat
      781 cgagaacgtg gtcaagacga tcgttcgttg cgccatcctt ggggaaagac ccttcatttt
      841 tccctggaac gtagaagacg gcctcgtgcg cgatattgat atctcttgcg atgtcctgca
      901 tcacgtcgga gtcttgtatc accttgttga tcctgttcgt cacctgcggg aggttctccc
      961 ttatgtgcgc gaggtcgtga tgctcgatac acacgtcgcg tctgcggcgg cggcagtaga
     1021 ttcgtatgtc tctggaaccg accggtttcg atatcagcga tatagggagg gggggcggga
     1081 agaaccgttt gcgggcatgg ccgaccatgc caagtggtta tgcgaagagg atctgctgag
     1141 ggtccttcgc gaagcagggt ttcaccatat cgatgtggct gagcgcaggc aggagagaaa
     1201 cggtcctcgg atcttgatct atgccagccg ctgaggcccg gagagaagtc gaacgaagtg
     1261 tgacgcgatg aaccttgtag acatggccac gcgcaaggat cgacacctcg agcttcaggc
     1321 ccgccttgtc tgcctaggaa atgacagatg gaaatcggcc aagatgctcg tgtcgcacgt
     1381 caccggtcgc tacatcatcg ccgcaaagcg gacgatcggg ggcatccagt atcgcggctt
     1441 tgacgtgtcg gccgacgacc gtacgagact catcgtccgg tccgcctgtg cgtggtatcc
     1501 cttccgactc ctgcccgttt caaccggttg gagcattgtt gccatggacc gtcgggcgga
     1561 agggaggcgt tttaatgcgg tgctgaaaga gcggtcaacg gaccgcgtcc tctccgcgag
     1621 gtcggtacgg ttgacgaaca cgctgcagcc tgtggcattt cctggtccgt ccggcgtccc
     1681 gattgacgcc acggaagccg atctccaact ggaattctgc ggcacggggg gcggaagagc
     1741 ttttctcgct gtgcatcgcg tgctggatcg aagcgaagtc atcgcattgt gccgcggaaa
     1801 aggcgtggaa cttggacctg ggacacaccc gcaggtgctg ccggacgaaa ccagagaggt
     1861 cctgtacgtc gaacagtcac agtctgacga gtggaacaca ttgtataacg aaaaaagacg
     1921 tctgcacgtc gatcaagagt cgtggtcgaa gtataccatc ggggaggcat tccctctccc
     1981 tgtagaagac ggttcattgg atttcatttt ttcaagccac gtcttcgagc acttggcgaa
     2041 tccgctcgga caccttgagc actggtatgc caaacttcgt cacggaggac tgatcgtcgg
     2101 catcgttcct gacgtaggag ggagcaagga ctatgtgttt gagccgtgct cggtcacggg
     2161 tttgctcgag gagcatgcct cgaagcagat gcagcctcag ctgagccact acagaaaatg
     2221 ggctgccgca cgtgcgccag gcaaggatcc ggcggaatat ttgaagtcaa aacgctcgat
     2281 ccacgttcat ttttacacgg ctgcgacgat gcagacgctg ctcgaacttg ctatcgctcg
     2341 gctgggctac agaacgtttc gcatctggca cacgccgaat cacaaggact tttattttgc
     2401 cttggaaaaa tgacggaacg aacgacatca gctgaggcat cggtgagagg actcgagtag
     2461 gtatgcgatg agcaatcggg cgacgatgcg acttgtccca tcagtgccta gactggccac
     2521 gagtaaggga ctggttcggc catcggccat tggcgagcgg ccagcggccg cgcagcttcc
     2581 tgaccatgct cacgtccgcg gattggatga gctacgtggt ctctccgtcc tctgggtgat
     2641 gctgtcccat gggactggat tgaccacatg gatgccgaca gcctttgccg ggtatggttt
     2701 gcacggagtc gtgctgtttt ttttgatcag cggctacttg attacacgca ttctggtgga
     2761 aagcaaggat cgtactgact acttcagtca cttttacatt aatcgtctat tcaggatttg
     2821 gccgctgatg ttcctggcat tattggtcag cgttctcatt tggcccgccc atgcgccttc
     2881 cgcagtctat aacctcctgc tcgtaaacaa ctatgcgtac gcgatgggaa ttgagccgcc
     2941 tgtccgcacg gacgtcatgt ggtctttggc gatagaacag caattttatc tgttctggcc
     3001 ggtcgcggtg tggctgtttt cagagaaggc ccttttgctc atcacctctt tgattgtgtt
     3061 gatcggattg ggagttgatg gagggttgct cccccaggga ggcgtcaaga ttgtccatgc
     3121 gtcgactctc ggctcgatgc agtatctcgg gatgggcgta ctggtcgcat tcggaaggaa
     3181 tggtctcaag tacctgctcg ggacgtggag tttgttcctg gcctggtggc tcacgcaatc
     3241 cgccatgccg gttttagagt ttcgctggat ttggtatggg ctgacgttca cgctcgcgtt
     3301 gcttgtatac cagaccatcc atgccaagcc gctgcttcaa tcccggtacc tggcttttac
     3361 cggagagcgc tgttatgggc tctacttgat ccattttttc gtgtccgcgt tggcatttat
     3421 cggcattgga aagggcgtct ggatggcagg cactgtctat ttcatcctgt cgtttcttct
     3481 cgctgtcatc tcattcaagt atttcgaacg gccgatccga agcttgcgcg tgcattttca
     3541 tcaaaacgta cgactccgag tcggtttatt tgcgggggtg ggcttcatga tccttgtaaa
     3601 cgtcgcctac ctggtgatca agtcaatagc ctaacgtccg gggacgttgt acgacgtcag
     3661 c
//
LOCUS       DASSCQ010000085        20559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_272321, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000085 DASSCQ010000000
VERSION     DASSCQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 20559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20559
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272321"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFH05_05960"
     CDS             complement(<1..906)
                     /locus_tag="VFH05_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET6677790.1"
                     /translation="MKVSLLFPPTWHPSQPYLSLPSLTGFLAQAGVTDVSQRDLGIEL
                     LDEVVTKSYGAGLYQELLDKKQALERERAGETGPGSAEHLARIIESLDRFPYLIDRIE
                     LAKETLRGEGFYDMEAYRESLFLIDKWLEVISTLYFPTRLTVVDNQFSTYSIYSSKDL
                     MKVIRDEQQNPYIKLFRERFLPSIVAGRPGLIGVSITATSQIIPGLTLCRLIKEAAPD
                     IHLTIGGSIFTRLVDNLRRCPNLFDITDDIVVFEGETALLELVNQMAGKKDFSKVPNL
                     IYRQNGKITVNQPFYSENVNQLPAPN"
     gene            complement(1120..2073)
                     /locus_tag="VFH05_05965"
     CDS             complement(1120..2073)
                     /locus_tag="VFH05_05965"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HET6677791.1"
                     /translation="MRVVQTHNKSVVFGILLSALIVGVMLTIGQVPLAVSQPVTIPAK
                     AVKGPIPMDGANPVWESVPGVIVPLSGQLITTPMHPNISVKSVFVKAMTNGKEVGLRV
                     EWSDQTKNDTTIGPQDFRDQAAIMFPVNTSGAPPFQCMGQSGGTTNIWRWNAEWQKDL
                     GKDSAGLWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNPGIWSGNIMSDPTL
                     RVSAVEDLNANGFSTLTTQAHQDVIGNGVWEPAGSVKGGGYSGPTWRVVVKRTLETGD
                     ANDVQFKAGMSVPIAFAVWDGNNIERNGMKALSTWFTLKLP"
     gene            complement(2169..3131)
                     /locus_tag="VFH05_05970"
     CDS             complement(2169..3131)
                     /locus_tag="VFH05_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HET6677792.1"
                     /translation="MTSKQSVQGSTQTSAAVISKSPIKDSPAVERALNRSKLYLLVSW
                     SLLYPEDDEFLDYLQCGEFVEDGRAALDALETALDGIGGDRAKQKLALMRKQFDQIEK
                     LIASECVNWQLSELQAEHRRVFSNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFYKA
                     FGVELSKDIHERLDHLSVEFEFMHFLAYKESYSRCHDSAEKTQIVVDAQKKFVKNHIG
                     RWVPLFCRMLIKKADSGLFKHVADMTSDWMDFEAAFLGVTPQPYSEADYRPATFNSPE
                     GQTYECGAQDQGNELNLLLNETGAQNFMDQKEKDKEEDGPVGTA"
     gene            complement(3350..3943)
                     /locus_tag="VFH05_05975"
     CDS             complement(3350..3943)
                     /locus_tag="VFH05_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6677793.1"
                     /translation="MAIDPKYGRRDFFKDSVISVAKAAKEFSSHADAVPEKPAPAMRT
                     DWLRPPGAVGEAIFLERCTKCSDCMKACPHESIVIHQDGTPVIYPDQMPCYLCDDTPC
                     IAACATEALLPVGSVNEARMGVAVINHRLCTAGQGCHACVSKCPTDALTMDFDAQRLV
                     VAVERCVGCGMCEHVCRTVNDHIAIRVTPFRLMESPR"
     gene            complement(4094..5092)
                     /locus_tag="VFH05_05980"
     CDS             complement(4094..5092)
                     /locus_tag="VFH05_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677794.1"
                     /translation="MELRFQPALLQEVIDSFVEKTEREGDPTYYKEFHELADPIYEKF
                     TLDDRESEFKKLYQYLFGTWGFSDIIRDAFNEYPLVKDKVGIVLVKGVLKEDQEGVDI
                     LRKWGSVEHDLARQFEEKGLKGIGIKLIPRRFYDPALTRYCRHELMHISDMLDPAFAY
                     DPDTKVGQNPGEETLILHRYRILWSLSVDSRLTAAGKEPMLRKEDRYKEFRSWYRKIP
                     AVQLKSVFEGLWQTGFFTHAELIEMATDTLRVMDRAVDVEGGEVPETENKVMLMPGFP
                     CPLCRFPTYTWVEDMGTKIETYVLDFIRENHPGWDVEFGACDRCVEVYKLRADGVM"
     gene            5298..6392
                     /locus_tag="VFH05_05985"
     CDS             5298..6392
                     /locus_tag="VFH05_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET6677795.1"
                     /translation="MTQPTASTPVPAQPLPVSAGMTTPLDSLEYWTADAREVRTFRGH
                     SHGVWSVAFAPDGLTLASAGVDRLVRIWDIETGRLLRSLRGHTNDIRAVLYTPDGQAL
                     ATGSEDRTIRLWNPKTGEPLKLLFTRYDHNICSLSLSPDGLMLARGSHNKDIKIWEVT
                     TGTELMTLLGKDQYDHHWSVCVAFSPDGRHLASGMDIGKIKIWEVLPSGEEKLVHNGH
                     WQETEEDSTETRGYFIEDDGGFQKPMDYWIGAMTFTPDAKYLITGSRDQTIKMFEMPE
                     ALEHRTLKGHGGWIRALVVTPDGKVLISSGDDNTIRFWDLKSGRCFRTIKSHTGGVRG
                     LSLSADGLRLASASWDRTVKLWEGGVEPAE"
     gene            complement(6696..8144)
                     /locus_tag="VFH05_05990"
     CDS             complement(6696..8144)
                     /locus_tag="VFH05_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6677796.1"
                     /translation="MKGMAILLVDDEPLIRLSMVDALEAVGYDVHAVASGTEGLNVLR
                     EKSFDLVITDLRLPGADGLTVLKATKDRSPETEVVMITAHGSVESAVGAMKLGAFDYI
                     TKPFQMDELLLIVERVGRVIALRRENRDLKETLESKFCFNGILGANSQMRGVLDKIKT
                     VAGTDSTILILGESGTGKELVANAIHQNSPRSQHPLIKVSCAALPETLLEAELFGHEK
                     GAFTGALRQRRGRFELAHRGTLFLDEIGEISPVVQVKLLRVLQERKFERVGGNETIDV
                     DVRLVCATQKDLRKEVSVGRFREDLFYRLNVVQVVIPPLRKRQEDIMVIADHVLQTCS
                     VKMNKKLKGYSTISRELLLRYSFPGNVRELENMVERAVALGRDREPVQPQDLCGFQSC
                     PFLGGTPQDTCGFCSEGLTGQKRPERPLNSLMSAREEFEKEYILSVLEKVEGSRTTAS
                     KILGLSRKALWEKCKRYGIPSAKDEVEDDEPK"
     gene            complement(8141..11320)
                     /locus_tag="VFH05_05995"
     CDS             complement(8141..11320)
                     /locus_tag="VFH05_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6677797.1"
                     /translation="MTFWSRSHSLQRKIIMAIVMVGLLPLTFLLGLTYFEERRALRET
                     TGINFKEVAVDAARRIEMHVTRVMNEGQQLATTPFLRTAVTEANRTYEGKDAYRVQEM
                     IKDWQQRWKQRDKRSEFPLFINRIVTNYLIRWHDIRKSDYVGILVTDQQGALVVSSIP
                     QVEYFYGKAPWWQALVRAASQEPYVSEITFDPSFGTHVVVVAAPILDDLQKTVIGAVT
                     ILLRRDTLFHSIADVTIGSTGHAMLFTSDGSPVICPILAPEEHSVKPELISVLGKLKS
                     GWTVAEDDSHGSKDALVGFAPVRFSQSLAAGSIGGKHWVTVVRQDPKETFAPLAELVA
                     KVLLYGLGVFAVLWGTGVLAARRIARPIQLLQGGVQEIGSGRLERRLELKTGDEIEEL
                     AGAFNEMASNLQRSFGQIEQRMREVRALEEKYRDLIEHSPEMIYQLDRSGQFVHVNKT
                     GLDKLGYQLSEMLMMKLWDVVPSGQAARVLQYLERLVSQGQTTMETVLIAKDGRLIDV
                     EIHATALFDEDRGGLVHSRAFVRDVTERHHLEQELQHYTTGLEQAVSERTQELVASQA
                     RYKALFDLVADSVFMVNAAGTVVAVNKREEQALGYAESKVVGRSMHDVVVPSHREALT
                     RWLAEIGTGQRQVPTQEITVLNAAGLETPVEMDLIRVAGAGQVLVMVQIRDITDRKKL
                     ERQLQIYREELEVKVRERTREIEETKQYLENLLENANDVIYTLDIEQRFTYVNSKVNV
                     WGYRKDDLLGRPYLSLLSRRHRGRRLKNTLDIGAKQVYEVEVMTRMGEARSVMVSVSP
                     LQGVDGEILGVLGIARDMTDTKKLEQQIRNSEKLASVGKLAAGVAHEINNPLAGILNC
                     LYNLRKGTLSPARQEEYWVSMEDGVRRVQKIVRQLLDFSQQHEPEFSSTDINQIVDRV
                     LVLTNHLFAPNLIRLETLLGPALPTLMVDRHMIEQVLMNLILNAVQAMKGGGVLTIRT
                     TVAEGVCRVEVSDTGSGIPPSVLPRIFDPFFTTKSEGEGTGLGLSVSLGIAERHGGKI
                     LVESEVGKGTTFTLCLPLSRERSLVERVS"
     gene            complement(11489..12406)
                     /gene="secF"
                     /locus_tag="VFH05_06000"
     CDS             complement(11489..12406)
                     /gene="secF"
                     /locus_tag="VFH05_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET6677798.1"
                     /translation="MLEILGKTNFDFMGKRRYAFLFSGIMVVLGIIGLIQIARGAANL
                     GIDFAGGTAVQLKFDQAIGIEEARKALETNGLGNAELQEFGQENKLLIRVKASTTIEE
                     KTAEKVVSIFTKEFPGNRFVVESSTEIGPTIGKKLQSDALVAIVISFAGIILYIAARF
                     ELRFGVAAALATFHDVLAVLGAFFILDKEITLLVVTALLTLAGYSLTDTVVVFDRIRE
                     NLRLRRREHEVTMVNNAINQVLSRTIVTSLTVVLVLIPLTLGGGEVLHDFSLALLWGV
                     IFGTYSSIFVASPLLLLWPGGAGRLLKRA"
     gene            complement(12422..14065)
                     /gene="secD"
                     /locus_tag="VFH05_06005"
     CDS             complement(12422..14065)
                     /gene="secD"
                     /locus_tag="VFH05_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET6677799.1"
                     /translation="MKKVRARLLLLLLVLLISIVCFLPSFPHLYLALPTWMKSIVPNK
                     GIALGLDLQGGIHMVLEVDEDRAVEIAVDRSVVAIQDLLVEKKIPAESVKRTSPTRIV
                     IQFQNADLKAQIQKLLDEFPTFTEVDSAGSGNTIAWELRETESKRIKDSAINQALETI
                     RNRIDQFGVAEPLVQRQGLKQIVVQLPGVRDPKRAKDLIKETALLEFKMLDEDNQMKL
                     DLPSRIPKDREAEVIKQAEAKLPEGSQVLFERAVERDTGREYRIPYLVKKRVMLTGDV
                     LSDARVSIGQFNDPYVSITFDAKGGREFERITGENVKKRMAIVLDNTIYSAPVIQERI
                     SGGRAQITGTFSTQEANDLAIVLRAGALPAPLKIIQDLTVGPSLGKDSIEKGVRATLI
                     AGAVVIGFMVVYYRLSGVIANFALVLNLICLMGALSALNATLTLPGIAGIVLTIGMGV
                     DSNVLIFERIREELRAGKAVRLAVDGGYDKALLTIVDSHVTTLITGVALFLFGTGPIK
                     GFAVTLCLGIAINLFTALVGTKVIFDIINQRHKVEQLSI"
     gene            complement(14093..14428)
                     /gene="yajC"
                     /locus_tag="VFH05_06010"
     CDS             complement(14093..14428)
                     /gene="yajC"
                     /locus_tag="VFH05_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET6677800.1"
                     /translation="MMLMESVAWAQGTGGSGSGSGTILSLVPFILIFVIFYFMLILPQ
                     QKKQKQQKAMMESLKKGDKVITSSGIWGTVTNLGKDTVTLQISDNTKIKMQREFIARV
                     RSDEDDKDA"
     gene            complement(14482..15639)
                     /gene="tgt"
                     /locus_tag="VFH05_06015"
     CDS             complement(14482..15639)
                     /gene="tgt"
                     /locus_tag="VFH05_06015"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET6677801.1"
                     /translation="MEFSVGQTDRLTRARLGTLTTAHGRMETPAFMPVGTLGPVKGLD
                     PEDLEQLGFRLMLNNAYHLYLRPGHKVVAEMGGLHRFTGWKGGILTDSGGFQIFSLAK
                     LCNVTDAGVSFQSHIDGSAHFISPEVAMEIQEALGADIIMAFDQCVALPADRIGVEEA
                     VIRTTRWAARCQASRHRRDQALFGIVQGGVDEELRIRSARDLVKLEFDGYAVGGLSVG
                     ESKDEMYRMLDVTVPELPVMKPRYLMGVGMPENLIEAVGRGIDLFDCVVPSRHGRTGW
                     LFTTFGRVLIKQARYQRDESPIDPACGCTVCARYSRAYLHHLYNVKEMLASRLNTLHN
                     LWFFSDLMRRMRVAIAQGTFPSFAESFYHQQSQEPAGRIASAKTGSDQSSC"
     gene            complement(15660..17411)
                     /gene="argS"
                     /locus_tag="VFH05_06020"
     CDS             complement(15660..17411)
                     /gene="argS"
                     /locus_tag="VFH05_06020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET6677802.1"
                     /translation="MSHGVVQEKVTSALLGALNGARNKGQLKSTAWPALSLDAPKRPE
                     WGDLASTVAMSLASSEQRAPHDIAQIIVENLPQRDQLFDRVEIVRPGFLNLTVKPALW
                     QETLKEIEAQGGLYGTSDLAKGRRALVEYVSANPTGPLHVGHGRGAAVGQALVRLLQA
                     VGYDVVSEYYINDAGRQMKLLGASVLARYRDQSGQRGDVPEDGYHGAYISALAERLKP
                     RLDAEGGLSLEQLEERCRTLAYQELLALIRDDLHAFGIDLASWFSEASLLSSQSVERV
                     LDELKSRGLVFEQDGAWWFRSTAFGDEKDRVVKKQDGEYTYLASDIAYHRDKLQRGYD
                     LLIDVWGADHHGYIPRMQAVMQAYGHPKERLQVVLVQLVKLLRAGEEVKMSKRTGEFI
                     TMREVIDEVGADAAKFYFLMRDSKTHLEFDLELAKQRSADNPVYYVQYAHARIASLWR
                     VAASRGIPCPLPSQTNLSLLSDPDELGLIRKLAAYPSTLEGAAVGYEPHRMTYYLQQL
                     AALLHTFYNKHRILPPAADAEVFTAATAGLSADEGAKRESLLPERTAARLALMRGVQQ
                     VIKNGLAVLGISAPDQM"
     gene            complement(17495..18502)
                     /locus_tag="VFH05_06025"
     CDS             complement(17495..18502)
                     /locus_tag="VFH05_06025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6677803.1"
                     /translation="MARTIVLFHPGALGDLLLAVPAIRRLRLCYPHHRLVLCAQEQAA
                     ALLGASNLVDEWVAFQAAGCAALFNGNRPEDPVLLDCLSRCDLAVAWIRDEAGMLATG
                     LKTCGAVDAVVQSPFAQALNAVHQSDRYAEILGEKVAGVSDISSLLMPENLCREAHAL
                     LQSCGVSLNRPFALLHPGSGSRHKCMNPAIWRCVTQALNEQGLLSLLLEGPADREVMQ
                     DLLDHLSPRPILLRELPLRLLAGVLTLADLFVGHDSGVTHLAALLGVPTVALFGPTDP
                     ARWAPRGPVVTVLQAQPCHCTSWDMVKNCGDKPCLNFSPSAILTACQAARAAGINPRN
                     T"
     gene            complement(18508..19194)
                     /locus_tag="VFH05_06030"
     CDS             complement(18508..19194)
                     /locus_tag="VFH05_06030"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET6677804.1"
                     /translation="MTRHGGGGETHPLLFFVSGRGDPMIADVTGVLLAGGKSRRMGED
                     KRFLSIGEETLYEHGLAVLRSVFQTVVVVIAQDSPPLASDVPVWRDLIPGCGSLGGLY
                     TGLKQAMTPSVFAVACDMPFLDPVTIRYFLTVQGEADLVMAKLPNGLHPMHALYHRRC
                     LPVMESLIREHDLKIQHLADHPSLRVRLVTADELVGIDPAGRSFYNVNTPADLRAARS
                     LHGKILPESS"
     gene            complement(19214..>20559)
                     /locus_tag="VFH05_06035"
     CDS             complement(19214..>20559)
                     /locus_tag="VFH05_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6677805.1"
                     /translation="RLQVLSFVTTQLVKDRKFTDKQSEAQTVLQLGDIKPIAATEESL
                     KKGSELIVEKKCVECHGAEGRGDGNAFNLKDDWGFAIQPADWHKCWNFRGSRQDPYNV
                     RNIFRTFSTGVNGTPMPSFADNTTIEERWHIANWVNFLCERDTDGKPLPIDPLTDKPK
                     INFVVPSDPVEGEIPTDMENEAWQKASKRYVAMGGQITHKPRNFVNRIDDIWVRSLYN
                     DKFIVYLLEWDDRTKSVAEGKLPWAPTQVNLDIKEQDPKTGEEGSIASNQNNYKVYND
                     AIAIETPVKWKELPAPIKPRYLFGTNEQFPVDIVKWEADGSIRAFKGTGWDKDFEERD
                     TYEENLKVLKGEWKNGRWYVMIQRPLGNKKDQDYDEDTFFEVGQYVPTVFFAWDGHNG
                     DAGRKMAVSAFYYTFLNPPIPKETYIYPAIIAFGVVLLEGWVMTRRANKKKGKTL"
BASE COUNT         4312 a         6215 c         5630 g         4402 t
ORIGIN      
        1 attgggtgcc ggcaactggt tgacgttctc ggaatagaat ggctggttga cggtgatctt
       61 gccgttctga cgataaatca gattcggcac cttgctaaaa tcctttttcc cggccatctg
      121 gttgacgagc tcgagtaggg ctgtctcccc ttcaaatacg acgatatcgt cggtgatatc
      181 aaagaggttg ggacaccgac gcaagttatc gaccagccgt gtgaagatgc tgccgccaat
      241 cgtcagatgg atgtcgggag cagcctcctt gatcagccgg cagagcgtca agcctggaat
      301 gatctgggac gtggccgtga tcgagacccc gatcaagcca ggacgcccgg cgacgatgga
      361 gggcagaaac cgttcgcgga acagtttgat gtacggattc tgctgctcgt cacgaatgac
      421 tttcatcaaa tccttggacg agtaaatgga ataggtactg aattgattgt cgaccacggt
      481 cagtctggtg ggaaaataca gagtggaaat gacttccagc cacttatcga tcaaaaagag
      541 gctttcccga taggcttcca tgtcgtagaa gccctcgccg cgaagcgtct ccttggcgag
      601 ctcgatccga tcgatcagat agggaaaccg gtcgagcgac tcgatgattc tggccagatg
      661 ctccgcgctg ccgggaccgg tctcgccggc tcgctcccgc tcaagtgcct gcttcttgtc
      721 aaggagctcc tgatacagac cggcgccata ggacttcgtg acgacctcgt cgaggagctc
      781 gatgcccaag tcgcgttgac tgacatcggt gacgccggct tgagccaaaa acccagtaag
      841 cgaaggaaga ctcagatagg gctgcgacgg gtgccaggtc ggtgggaaaa gcagtgaaac
      901 tttcatgcca aatctctacc gagagaattg aaaaataaga taaatctcga aagataagga
      961 ctttgcttta tacactccgc ccctgctcaa atgcaaccca ccctggtcgt caacagcccc
     1021 cataaataga aaagccccag ctcaagagaa ctggggcttt tctttggctg tctgttccac
     1081 cggggaaagg tggaacatgg tcactcagac ctgttcagct cacggaagct tgagcgtgaa
     1141 ccaggtggag agcgccttca tgccattacg ctcgatgttg ttgccgtccc acaccgcaaa
     1201 tgcgatgggc acagacatgc ccgccttgaa ctgcacgtcg ttggcatcgc ctgtttccag
     1261 ggtccgcttc acgactacgc gccaggtcgg gccgctataa ccgcctccct tgacggaacc
     1321 ggccggttcc cacacaccgt tgccgatcac gtcctggtga gcctgagtcg tcaaggtgct
     1381 gaagccgttg gcgttcagat cctcgaccgc gctcacgcgc agggtcgggt cagacataat
     1441 gttgcccgac caaatgccgg ggttgaaggg tccaaggctg cggccgatcc ggtccggata
     1501 ggtcacgccg ccggcaggct cttcaaaata gtagtcccag aagattcccg gatattgatc
     1561 gtccacgtcc cagagtcccg cgctatcctt gccgaggtct ttctgccact ctgcgttcca
     1621 acgccaaatg ttcgtggtgc cgcctgattg acccatgcac tggaagggcg gcgcaccgga
     1681 cgtgttgact gggaacatga tcgcggcctg gtcacgaaag tcctggggac cgatcgtcgt
     1741 gtcgttcttg gtctgatcgc tccactcgac gcgcaacccg acttccttgc cattcgtcat
     1801 ggccttgacg aacaccgact tgaccgagat gttcggatgc atcggcgtgg tgatcagctg
     1861 gccgctcaat ggcacgatga caccggggac gctctcccac acaggattcg ccccatccat
     1921 cgggatgggt cccttgaccg ccttcgccgg gatggtcacc ggttgactga cggccaacgg
     1981 cacctgccca atcgtcaaca tcacgccaac gattagggca gagagaagaa tgccaaacac
     2041 cacagacttg ttgtgtgtct gcactactct catggactat gccctcctct caattaaatg
     2101 attaagactg acccaaaata gctgcctcaa acggtagaaa aaaggaaccc ccgccaccga
     2161 acggattatt aggccgtccc gaccggcccg tcctcctcct tgtccttttc cttttgatcc
     2221 atgaagttct gcgcgccagt ttcgttcaat aggaggttga gttcattgcc ttggtcctgc
     2281 gcgccacact cgtaggtctg gccttcaggt gaattgaacg tcgcgggacg ataatcagcc
     2341 tccgaatagg gttgcggcgt gacaccgagg aacgcggctt cgaaatccat ccagtcggaa
     2401 gtcatgtccg cgacgtgctt gaacagaccc gagtcggcct tcttgatcag catgcggcag
     2461 aacaacggca cccagcgtcc aatgtgattc ttgacaaatt tcttttgagc gtcgacgacg
     2521 atttgggttt tttcggcgct gtcatgacag cgggagtagg attctttgta ggccagaaag
     2581 tgcatgaact cgaattcgac gctcagatgg tcgaggcgtt catggatgtc tttggagagc
     2641 tcgacgccga aggccttata gaatcccgcg atgtcgccca tgacgtggga ttgcgcgaag
     2701 acgtgatcgt tgccaaagag ggtttcgtag ggaggacagt ccagcgtgat gacgttgctg
     2761 aacacgcgcc ggtgttcggc ctgcaattcg ctcagttgcc agttcacgca ctcggacgcg
     2821 atgagctttt cgatctgatc gaactgcttg cgcatcagtg ccagtttttg cttcgctcga
     2881 tcgccgccga ttccgtcgag tgcggtctcc agcgcatcga gcgcggcacg accgtcttcc
     2941 acgaactccc cgcactgcaa atagtccaga aattcgtcgt cttcaggata gagcaagctc
     3001 caggacacga ggagatagag tttgctgcgg ttgagggcgc gttcgacggc aggggaatct
     3061 ttgatggggg atttcgaaat gacggcggct gaggtctgtg tagaaccttg taccgactgc
     3121 ttactcgtca taagaatata ctcctcgctt cgaccaagtt ttctgacatt tcggggataa
     3181 cggaggtcaa acccctggcc aaagcctggg tatgataggt aaagggtgag ggcttgtcaa
     3241 gcatgattct gttccaccga gtcatctgta agacgtggtt ggtttcccga catttcggcc
     3301 gctcatggtt acggtctggc cgccgaacgt ggctgctttc tacactgaat tagcggggcg
     3361 attccattag acgaaagggt gtgaccctga tggcgatgtg atcgttcacg gtgcgacaaa
     3421 cgtgctcgca catcccgcat cctacacaac gttcaacggc gactacaagc cgttgggcat
     3481 cgaaatccat ggtcaatgca tcggtggggc atttcgacac acaggcatgg cacccctggc
     3541 ctgcggtgca aagtcgatgg ttgatcacag ccactcccat cctggcctcg ttgacactgc
     3601 ccacgggcag gagggcttcg gtcgcacagg cggcaatgca aggcgtatcg tcacagagat
     3661 agcacggcat ctgatcgggg taaatgaccg gcgtgccgtc ctgatgaatg acaattgact
     3721 cgtgtgggca ggctttcatg caatcactgc acttcgtgca gcgctcgaga aagatcgcct
     3781 cgccgacggc accaggagga cggagccagt ccgtgcgcat ggccggagcc ggcttttcag
     3841 gaactgcgtc cgcatgggaa gaaaactctt tcgcggcttt tgcgaccgag atgaccgagt
     3901 ccttgaaaaa gtcccgtcga ccgtatttgg gatctatagc catggtattc gtcggttatt
     3961 atcgctgagc agatggtcaa aacgcccagg cagcaaggcc gcagcgagcg agaggccgag
     4021 gcgtactttc acttgtacgt cgaggtctga gcgatgcgag aacgcagctg atgggcgttt
     4081 tcaccatctg tcgttacatg acgccgtccg cccgaagctt atacacctcg acgcagcgat
     4141 cgcacgctcc gaattccacg tcccagccgg gatgattctc gcggatgaag tcgagcacat
     4201 aagtctcgat cttggtcccc atgtcttcta cccatgtata ggtcgggaac cgacatagag
     4261 ggcagggaaa cccgggcatc aacatgacct tgttttccgt ctccggcact tcaccgcctt
     4321 ccacatccac cgcgcggtcc atcactcgca acgtgtcggt cgccatttcg atcagttcag
     4381 cgtgggtgaa gaaccccgtt tgccacaagc cctcgaacac ggacttcaac tgcaccgcgg
     4441 gaattttccg gtaccacgaa cgaaattctt tataccggtc ttccttgcgc agcatcggct
     4501 ctttgccggc ggcggtgagc cggctatcga cactgagact ccataaaatg cgatagcggt
     4561 gcaggatgag ggtttcttcc ccgggattct gtcccacctt ggtatcggga tcgtaggcga
     4621 aggctggatc gagcatatcc gagatgtgca tcagctcatg ccggcaatac cgcgtgagcg
     4681 ccggatcgta aaaccggcga gggatgagct tgataccgat gcctttcagg cccttttcct
     4741 cgaattgcct ggccaggtca tgttcaaccg agccccattt tcgtaaaata tcgacacctt
     4801 cctggtcttc ctttagcaca cctttcacga gaacgatgcc aaccttgtcc ttcaccagcg
     4861 gatactcgtt gaaggcatca cggatgatgt cagaaaaccc ccacgtgcca aagagatact
     4921 ggtaaagctt tttgaactcg ctttcccgat cgtcgagggt gaacttttca taaatcgggt
     4981 cggccagctc gtggaactcc ttgtagtagg tcggatcacc ctcccgctcg gttttttcca
     5041 cgaacgagtc gatcacttcc tgcagcagtg caggctgaaa acgcagttcc atatcaccct
     5101 cgagtttttc cgattccggg gccgctttcc ccagtcgtga tgcggtttgg cggcgccttg
     5161 agcggcttga cttggcgccg ttgcttctct tacgatcacg gaaacgaccg caaattatac
     5221 tgggccgaaa cggcgatttg caatcaagcc accgtccggc cgcttcacca tttacgattc
     5281 gggcaaggta acgactcatg acacaaccaa ccgcatccac tcctgtccct gcgcagccac
     5341 tgccggtttc agccggcatg acgactcccc tcgattcgct tgaatactgg acggcggacg
     5401 cccgcgaagt gcgaacgttc cgcggacatt cgcatggcgt ctggtccgtc gcctttgcgc
     5461 cggatggctt gacgttggcc agtgccggtg tcgaccgtct tgtccggatc tgggatattg
     5521 aaaccggccg gctgctccgc tcgcttcggg gccacaccaa tgacatccgg gcggtgctct
     5581 acacgccgga tggacaagca ttggccactg gcagcgaaga ccgaacgatc cgcttgtgga
     5641 atccgaagac cggcgaaccg ctgaagttgc tgtttacccg atacgatcac aatatctgca
     5701 gtctgtcgct gtctccggac ggcttgatgc tggcacgggg cagccataat aaggacatca
     5761 agatttggga ggtcacgacc ggcacggagt tgatgacctt gctcgggaag gatcaatacg
     5821 atcaccactg gtccgtgtgc gtggcgttct cacccgacgg cagacatctc gcaagcggaa
     5881 tggacatcgg caagatcaag atctgggagg tcctacccag cggcgaagag aagctcgtgc
     5941 acaatggtca ttggcaggag accgaagaag actccactga aacgcgtggg tacttcattg
     6001 aggacgatgg tggttttcaa aagcctatgg attattggat cggcgccatg acgttcaccc
     6061 ccgacgccaa gtatctcatc accggtagcc gcgatcagac gatcaagatg tttgaaatgc
     6121 cggaggcgct tgagcatcga acgctgaaag ggcacggcgg ctggatccgt gcgctcgtcg
     6181 tgactcccga cggcaaagtc ttgatcagct ccggagacga caacacgata cgcttctggg
     6241 acctcaaatc aggccggtgc ttccggacga tcaaatctca caccggcggc gtccgcggtc
     6301 tctcactctc ggcggacgga ctcagactcg ccagtgcatc ttgggaccgg acggtgaagc
     6361 tgtgggaggg tggggtcgaa ccagcagagt aacccatggc cgcgcgaagg tttggcgcgg
     6421 aaggtgggtt gctgctcagg cgatgggtga aaccgcatgc cctctcgctg cttggctccg
     6481 caaacttggt ccgtcctgga cgttgtccga acggaccttc gaacactgcg tctcgccatc
     6541 gcgcaacgcg accgcaacga cgggccatgc gacccaccgc aagtcgcagc taaccccacc
     6601 acaccgtttc acctaaaaaa cgaagaggcg acaggcccct tcaagcctgt cgcctctttt
     6661 acttttttct caccctctag cgctcggcgc tcgattcact tgggctcgtc gtcctccacc
     6721 tcgtccttgg cggaggggat gccgtagcgc ttgcacttct cccagagcgc cttccgtgag
     6781 agcccgagaa tcttcgacgc ggtcgtgcgg ctgccttcca ctttttccaa caccgagaga
     6841 atatactctt tctcgaattc ctcccgagcc gacatcagtg aattgagcgg ccgctccggc
     6901 cgcttctgcc ccgtgagacc ctcgctgcaa aatccgcacg tgtcctgagg cgtgccgccc
     6961 agaaacggac aggattggaa gccgcacaag tcctgcggct gaaccggttc gcggtcgcgt
     7021 cccagcgcca ccgcccgctc gaccatgttt tcaagctctc tcacattacc cggaaacgaa
     7081 tagcgcagta ggagctcacg tgaaatagtg gaatatcctt taagtttctt attcatcttc
     7141 accgaacagg tttgcaagac atggtcggcg atgaccatga tatcttcctg ccgtttccgc
     7201 agcggaggta tcaccacctg cacgacgttc aaccgataga ataaatcctc gcggaaccgt
     7261 ccgacggaga cttccttgcg gagatccttc tgcgtcgcgc agaccagccg gacgtcgacg
     7321 tcgatcgtct cattgccgcc tacccgctcg aacttgcgct cctgcagcac acgcagcagc
     7381 ttgacctgca cgaccggcga gatctcgccg atttcgtcga gaaagagggt gccccgatgg
     7441 gccagttcaa accgtccgcg acgttgccgt aatgctccgg tgaaggcacc cttttcatga
     7501 ccgaacagtt ccgcctcgag cagagtttca ggcaaggccg cgcagctgac cttgatgagc
     7561 gggtgttggc tccgcgggct gttctggtga atggcattgg cgaccaattc tttgcccgtc
     7621 ccgctttcgc ccaggatcag aatggtcgaa tcggtcccgg ccacggtttt gatcttgtcc
     7681 agcacccccc gcatctggct attggcgccg aggatgccgt tgaagcagaa tttgctctcc
     7741 agcgtttctt tcaggtcgcg attctcacgc cgcaacgcta tgacgcgccc gacacgctcg
     7801 acgatcaaga gcaactcgtc catctgaaac ggtttggtaa tataatcgaa tgcgccaagc
     7861 ttcatagcgc ccaccgccga ttccaccgac ccgtgcgcgg tgatcatcac gacttccgtc
     7921 tcgggcgatc tgtccttggt ggctttgaga accgtaagcc catcggcgcc tggcaaccgc
     7981 aggtcggtaa tgaccaggtc gaacgatttc tcgcgcagca cgttgagacc ctcggtgccg
     8041 gacgccacgg catgcacgtc gtaccccacc gcctccagcg catcgaccat cgagagccgg
     8101 atcagcggct catcgtcgac caacaggatg gccatccctt tcatgacact ctttccacca
     8161 aggaccgctc tctcgacagc ggaagacata atgtaaacgt cgttcctttc ccgacttcgc
     8221 tctctaccag gatcttacca ccatgtcgct cagcaatgcc cagactgacc gacaagccca
     8281 ggccggtccc ttcgccctca ctcttcgtgg tgaagaaggg gtcaaaaatg cgcggcaata
     8341 cagacggggg aatccctgac cccgtgtcgc tcacctcgac tcggcagacg ccctcggcaa
     8401 cggtggtccg tatcgtcaac acgccgccgc ctttcatcgc ctgcacggcg ttcaagatca
     8461 gattcatcaa cacctgctcg atcatgtgcc gatcgaccat gagggtcggc aaggcaggac
     8521 ccagcagagt ttccagccgg atcaaattgg gtgcgaacag atgatttgtg agcacgagta
     8581 cccggtcgac gatctggttg atatcggtgg agctgaactc cggctcgtgc tgctgagaaa
     8641 aatccaggag ttgccggacg atcttttgca cccgccggac accgtcttcc atcgaaaccc
     8701 aatattcttc ctgacgcgcc ggagaaagcg tacctttgcg gaggttgtac aggcagttca
     8761 aaatgccggc gagcgggttg ttgatctcgt gggcaacgcc ggcggccagc tttcccacgg
     8821 atgcgagctt ctccgagttc ctgatctgtt gttcgagctt cttggtgtcc gtcatgtcgc
     8881 gcgcaatgcc gagcacgccc aggatctctc catcgacacc ctgcagcggg gagacgctga
     8941 ccatgacgct ccgtgcttcg cccatccgcg tcatgacttc gacctcatag acctgtttgg
     9001 cgccgatatc caacgtgttc ttgagacgtc gcccgcgatg ccggcgcgac agcaacgaga
     9061 gatacggacg tccgagcaga tcatccttgc ggtaccccca cacattcacc ttgctgttga
     9121 cgtacgtgaa ccgttgttcg atgtcgagcg tatagatcac gtcgttggcg ttctcaagca
     9181 ggttttccag atattgtttc gtttcctcga tttccctggt ccgttcacga actttgactt
     9241 cgagttcttc gcgataaatt tgcagctgtc gttcgagctt cttccggtcg gtgatgtcgc
     9301 gaatctgcac catcaccaac acttgacctg ctccggcaac gcggatcagg tccatctcga
     9361 cgggcgtttc caaacccgcc gcgttgagga ccgtgatttc ctgggtcggg acctgccgtt
     9421 gaccggtgcc gatttcggcc agccagcggg tcagagcctc ccgatgcgac ggcacgacga
     9481 cgtcgtgcat gctgcgtccg accaccttgg actcggcgta cccaagcgcc tgctcttcgc
     9541 gcttattgac ggcgacaacg gtcccggcgg cattcaccat gaacaccgag tctgcgacca
     9601 ggtcgaacaa ggccttgtaa cgggcctgag atgcgaccag ttcctgcgtg cgctcggaaa
     9661 ccgcctgctc caagccggtg gtgtaatgct gcagttcctg ctccaggtga tgccgctccg
     9721 tgacgtcgcg tacaaacgca cgggaatgca ccagtccgcc gcgatcctca tcgaagaggg
     9781 cggtggcatg aatctccacg tcgataagac ggccgtcttt cgcgatcagg acggtttcca
     9841 tcgtagtctg cccttgagag acgagccgct cgagatactg cagcacgcgc gccgcctgtc
     9901 cgcttgggac aacatcccag agcttcatca tcaacatttc gctcagctga taacccagct
     9961 tgtcgagacc ggtcttattg acgtgcacaa actgtccgct gcggtccaac tggtagatca
    10021 tctcgggcga atgctcgatc agatcccggt acttctcttc caatgcccgc acttcccgca
    10081 tccgctgctc gatctgcccg aatgagcgct gcaggttcga agccatttcg ttgaacgcac
    10141 cggccaattc ctcgatttca tcccctgtct tgagctcaag acggcgctcc aaccgcccgc
    10201 tgccgatttc ctgcactccg ccctgcaaca attgtatcgg tcgagcgatc cgccgggccg
    10261 caagcactcc ggttccccac aagacggcga agacaccgag accatagagc aggactttgg
    10321 ccacgagctc cgccagcggc gcgaatgttt ctttaggatc ttgccggaca acggtcaccc
    10381 aatgcttgcc gccgatgctt cccgccgcga gcgactgact gaaccgcacc ggggcaaaac
    10441 cgacgagggc atccttgctt ccgtgcgaat cgtcctccgc aaccgtccag ccggacttca
    10501 gcttgcccag cacactgatc aattctggct tcaccgaatg ttcttccggt gccaagatgg
    10561 gacagatgac cggactcccg tcagatgtga aaagcatcgc atgaccggtc gacccgatgg
    10621 tgacgtctgc aatcgaatga aacaacgtgt cccgtcggag cagaatcgtg acggctccaa
    10681 taaccgtctt ctgaagatcg tctagaatcg gggcggcgac cacgaccacg tgggtaccga
    10741 acgagggatc gaaggtgatt tcactgacat agggctcttg gctggcagcc ctgaccaatg
    10801 cctgccacca gggggctttc ccgtagaagt attcgacctg gggtatcgag ctcacgacca
    10861 gtgctccctg ctggtcagtc accaggatcc cgacatagtc cgacttccgg atgtcgtgcc
    10921 aacggatgag ataattggtg acgatgcggt tgatgaacag ggggaactcg ctgcgcttgt
    10981 ctcgttgctt ccagcgctgc tgccagtcct tgatcatttc ctggacgcgg taagcgtctt
    11041 tgccttcgta ggtccgattg gcctccgtga ccgcagtgcg caggaacggc gtggtggcaa
    11101 gttgctggcc ttcgttcatg acccgcgtca cgtgcatttc gatccggcgg gcggcatcga
    11161 cggcgacttc cttgaagttg atgccggtgg tctcgcgcaa cgcgcggcgt tcttcgaaat
    11221 aggtgaggcc aagcaggaac gtcagcggaa ggagcccgac catcacgatg gccatgatga
    11281 tcttgcgctg gagactgtga gagcgactcc agaatgtcat gataggtcgg cctagtcttg
    11341 aaaacccagt ttcagcggcg actcatctta cctggtgatc agaaagcgca tcaatctcgt
    11401 cttcgcgatg tgagaacgcc agccggtgaa aggcgctctt ggcgcgtcgg ggttggcggg
    11461 tctgacttag gccctttcca acaccctatt aagcccgctt caggagccgt ccggcccccc
    11521 ccggccacaa caacagcagc gggctggcga cgaagatcga ggaataggtg ccgaagatga
    11581 cgccccaaag caacgccagc gagaagtcat gcagcacctc cccgccgccc agcgtcaacg
    11641 gaatcaacac caagacgacc gtcagactgg tgacaatcgt gcggctcaac acctgattaa
    11701 tcgcattgtt gaccatcgtc acttcgtgct cacgacggcg cagacgcaag ttctcacgga
    11761 tacggtcgaa gaccacgaca gtgtcggtga gcgaatagcc tgcgagggtt aacaacgctg
    11821 tgacgaccag cagggtgatt tctttgtcga gtatgaagaa ggcccccaac accgcgagga
    11881 cgtcgtgaaa cgtcgccagc gccgccgcca cgccgaaccg caactcgaac cgcgctgcaa
    11941 tgtagaggat gatgcctgcg aatgaaatga cgatggcgac gagtgcatcc gactgcaact
    12001 tcttgccgat cgtcggcccg atttccgtgc tcgactctac cacgaagcgg ttccccggaa
    12061 attccttggt aaaaatcgat accacctttt ccgccgtttt ttcctcgatg gtcgtcgagg
    12121 ccttcacacg gatcagcagc ttgttctcct ggccgaattc ctgaagttcg gcattgccca
    12181 ggccgttggt ctcaagcgcc tttcgcgctt cctcgatccc gatcgcctga tcgaacttca
    12241 actgcacggc tgtgccgccc gcaaagtcga tgcccaaatt ggcggctccc cgagcgatct
    12301 gaatcaatcc gatgatgcca agtaccacca tgatgccgga gaacagaaac gcataccggc
    12361 gcttacccat gaaatcaaag ttcgtcttcc cgagaatttc caacatgcag gctccttcac
    12421 actaaatgct cagctgctca actttgtgac gttgattaat gatgtcgaag atgaccttgg
    12481 tcccgacaag ggcggtaaac aggttgatgg caattcccag gcagagcgtg acggcgaatc
    12541 ccttgatcgg cccagtcccg aacaaaaaca gcgccacacc ggtgattaat gtcgtcacgt
    12601 gcgaatcgac aatcgtcagc agcgctttgt cgtagcctcc atcgaccgcc aatcggaccg
    12661 ccttccctgc ccggagttct tcccggatgc gttcgaagat gagcacgttg gaatcgacgc
    12721 ccatcccgat cgtcagcacg atgccggcaa tgccgggcaa cgtgagcgtc gcgttcagcg
    12781 ccgacagcgc ccccatcaga cagatcagat tgaggacgag cgcaaagttc gcgatcacac
    12841 cggaaagccg gtaatacacg accatgaagc cgatcaccac ggcccctgca atgagggtcg
    12901 cccggacgcc cttttcaatc gaatctttcc ccagtgatgg gccgaccgtc agatcctgaa
    12961 tgatcttcaa cggcgccggc aacgcgccgg ccctcaacac gatcgcgagg tcattggcct
    13021 cctgcgttga aaaagtgccc gtgatctgcg ccctcccgcc gctgatccgc tcttggatga
    13081 ccggcgccga atagatggtg ttgtcgagga cgatggccat ccgcttcttg acgttttctc
    13141 ccgtaatccg ctcgaattcg cgtccgcctt tagcgtcaaa ggtaatggac acgtatggat
    13201 cgttgaattg gccgatcgac actcgcgcat cgctcaagac gtctccggtc agcatgaccc
    13261 gctttttgac gaggtaggga atacggtact cacgtccggt gtcccgttcc acggcccgtt
    13321 cgaagagaac ctgactgcct tcaggcagct tcgcttctgc ctgcttgatg acctccgctt
    13381 ccctgtcttt cggaatgcgg gagggcagat cgagcttcat ctggttgtcc tcatccagca
    13441 ttttgaattc aagaagggcg gtttccttaa tcagatcctt cgcccgcttg ggatcccgaa
    13501 cccccggcaa ctgaacgacg atttgcttca ggccctgccg ctggacaagg ggttccgcga
    13561 cgccgaattg gtcgatacga ttacgaatcg tttccaaggc ctggttgatg gcggagtctt
    13621 tgatgcgctt gctttccgtc tctcggagct cccaggcgat cgtgttccca gagccggcgg
    13681 agtcgacttc ggtgaaggtg ggaaattcat cgagcagctt ctggatctgc gccttcaagt
    13741 ccgcattctg gaattggatg acgattctcg tcggcgacgt ccgtttcaca gattcggccg
    13801 ggattttctt ctccaccagc aaatcctgta tggccaccac cgaacgatcc accgctattt
    13861 ccacggcccg atcttcgtcg acttccagga ccatgtggat gccgccctgc aaatccaagc
    13921 cgagcgcgat gcccttgttc ggcacgatgg atttcatcca cgtcggcaac gcgagataga
    13981 gatggggaaa ggacggcaag aagcacacga tggaaatcag caggaccagc agcagtaaca
    14041 acagtctcgc acgtactttt ttcatatcga gtccaatccc tccgtccttc ggctacgcat
    14101 ctttgtcgtc ttcgtcagag cgtacccgcg caatgaattc ccgttgcatt ttgattttcg
    14161 tgttatcgga gatctgcagc gtcacagtgt cctttcccaa attcgtgacc gtgccccaga
    14221 tgccagacga tgtgatcact ttgtcgcctt tcttcagcga ctccatcatg gctttttgtt
    14281 gtttctgctt tttctgctgc ggcagaatca acatgaaata gaagatgacg aagatcaaga
    14341 taaagggcac cagcgagagg atcgtccccg accccgaccc gctgccaccc gtgccctgtg
    14401 cccacgcgac cgattccatc agcatcatga tgttcctctc ttaccaggcg ctggtcttat
    14461 ccaactcaca cccctcaaca ttcagcagct cgactgatcg ctccccgtct ttgccgatgc
    14521 gatcctgccg gccggctctt ggctctgctg atgataaaag gattcggcaa acgacgggaa
    14581 ggtaccctgt gcgatcgcca cgcgcatccg ccgcatcaag tcggaaaaaa accataagtt
    14641 atggagggta ttgagtcggg aagcgagcat ctccttcacg ttgtacagat gatgcaggta
    14701 cgcgcgagaa tagcgcgcgc acacggtgca gccgcaggcc ggatcgatag gcgattcgtc
    14761 tcgttgataa cgggcttgct tgatcaacac ccgtccaaaa gtggtaaaca gccatcccgt
    14821 tctgccatgc cgcgacggca cgacgcagtc gaagagatcg atgccgcgcc ccaccgcttc
    14881 aatgagattc tcaggcatgc ccacgcccat caaatagcgt ggcttcatca ccggaagctc
    14941 cggaacggtg acgtccaaca ttcgatacat ctcgtccttg ctttcgccga ccgagagccc
    15001 tccgacggca tagccgtcga attccagttt caccagatcg cgggcggagc gaattcgaag
    15061 ttcttcatcc actcctcctt gcacgatgcc gaacagcgct tgatctctgc gatgccggct
    15121 ggcctgacag cgtgccgccc accgagtcgt ccgtatgacc gcctcctcga cgccgatccg
    15181 gtcagcgggc aaggcgacac attggtcgaa tgccatgatg atgtcggcac cgagcgcttc
    15241 ttgaatctcc atcgccactt cgggcgagat gaaatgtgcg gacccgtcaa tgtgcgattg
    15301 gaaactcaca cccgcgtcgg tcacgttgca gagcttggcc aggctgaaaa tctgaaaacc
    15361 tccgctgtcg gtcaggatgc cgcctttcca accggtaaat cggtggagcc cgcccatttc
    15421 ggccacgacc ttgtggccgg gacgcaaata caagtggtag gcattattca gcatgagccg
    15481 gaagcccagc tgctccaggt cttcagggtc aagtcccttg acgggaccca gcgttccaac
    15541 cggcatgaat gccggcgtct ccatccgccc gtgggctgtg gtgagcgtgc cgagtcttgc
    15601 gcgcgtcaac cgatccgtct ggccaaccga aaactccaca atccctcttc cgattcggct
    15661 cacatctgat caggggcaga aatcccgaga accgccaggc cgttcttgat cacttgctgg
    15721 actcctcgca tcaaggctaa gcgagcggcg gtccgctcgg gaagcaacga ttcccgtttc
    15781 gccccttcat cggctgagag gccggccgtc gctgccgtga agacctcggc gtcggccgca
    15841 ggaggcagaa ttcggtgttt gttataaaac gtgtgcagga gcgcggcgag ctgctgcagg
    15901 tagtacgtca tgcggtgcgg ctcatatccc acggcggcgc cttcgagcgt cgacggatag
    15961 gcagccaatt tcctgatcaa ccctaactcg tccggatcgg acaacaagga gagattcgtc
    16021 tggctgggca acgggcaagg gatgccgcgc gaggcggcaa cccgccagag actcgcgatc
    16081 ctggcatggg catattgtac gtagtagacg gggttgtccg ccgatcgctg cttcgccagc
    16141 tcgagatcga attccaagtg agtcttcgaa tcacgcatga ggaaataaaa tttggccgca
    16201 tcggctccga cctcgtcgat gacctcgcgc atcgtaatga actcgccggt gcgcttcgac
    16261 atttttactt cctcgccggc tcgcaagagc ttgaccagct gcaccaacac gacttgcagc
    16321 cgctctttgg gatgtccata ggcctgcatg accgcctgca tgcgcggaat gtagccgtgg
    16381 tgatcagcgc cccagacatc gatcagcagg tcgtagccgc gctggagctt atcgcggtga
    16441 tacgcgatgt cggaggcgag gtaggtgtat tcgccgtcct gtttcttgac cacccgatct
    16501 ttctcgtctc caaatgccgt agaccgaaac caccaggcgc cgtcctgctc gaacaccagc
    16561 ccgcgggatt tcagttcatc cagtacgcgc tcgaccgatt gtgaggacaa taaggaagcc
    16621 tcgctgaacc aggaagccaa atcgatcccg aacgcgtgta gatcatcgcg gatcagtgcc
    16681 agcaattcct gataggccag tgtccggcac cgctcctcaa gctgttcaag cgacaacccg
    16741 ccttccgcat caagccgcgg tttcagccgc tcggccaggg cggaaatata ggctccatgg
    16801 tagccatcct cgggaacgtc tcctcgctgg ccggattggt ctcgataacg ggccaagacc
    16861 gacgctccca acagcttcat ctgacgcccc gcgtcgttga tgtagtattc gctgacgacg
    16921 tcatagccga ccgcctgcag taggcggacg agggcttgtc cgacggcggc cccgcggccg
    16981 tgtccgacgt gcaatgggcc ggtcggattc gcgctcacgt attccaccag cgcccgccgt
    17041 cccttggcga gatcacttgt cccgtacaac cctccctggg cctctatctc tttaagggtt
    17101 tcctgccaga gcgcgggctt aacggtgaga ttcaggaatc cggggcgaac gatttccacc
    17161 cggtcgaaga gttgatcccg ttggggaaga ttttcaacga tgatctgcgc gatgtcgtga
    17221 ggcgctcgtt gctcagagga cgcaagggac atggcgacgg tcgaggcgag gtctccccat
    17281 tcgggccgct tcggcgcatc gaggctgagc gccggccagg ccgtgctttt caattgtcct
    17341 ttgttcctgg ctccgttcag ggcgccgagc aacgcactgg tcaccttttc ctgcactacg
    17401 ccgtgggaca aaatgtctcc taagtcctag cattgaaaag agaaaattaa ctcggcactc
    17461 tatcataggg tggaattgcg agacaaggca caggtcaggt atttcgtggg tttatgccgg
    17521 cagctcgagc cgcctgacag gcagttaaaa tcgcgctcgg cgaaaagttc aggcacggct
    17581 tgtctccaca gtttttcacc atgtcccatg acgtgcagtg gcaaggctgt gcttgtaaga
    17641 ccgtcaccac aggccccctc ggtgcccatc gcgccggatc ggtcggcccg aacagggcca
    17701 cggtcggcac tcccagcaag gctgcgaggt gagtgactcc cgagtcatgt ccgacaaaca
    17761 gatcagccag cgtgagcacc ccggccagca ggcgtagcgg tagctcacgc aacagaatcg
    17821 gtctgggcga cagatggtcc agcagatctt gcatgacctc gcgatcagcc ggtccttcga
    17881 gcagcaggga cagtagccct tgctcattca gggcttgcgt gacacatctc catatcgcgg
    17941 ggttcatgca cttatggcga cttccgcttc ccggatggag aagcgcgaag ggccgattca
    18001 atgaaactcc gcagctctgc agaagagcgt gtgcctcccg gcaaaggttc tcaggcatga
    18061 gcagggagga gatatccgac acaccggcca ctttctctcc aaggatttca gcataacgat
    18121 cgctttgatg gaccgcgttc aaggcctgag caaacggcga ttgaaccacc gcatcgaccg
    18181 ctccgcacgt cttaagccca gttgcgagca tgcccgcttc atctctgatc caggcgacgg
    18241 cgaggtcaca gcggctcaag caatcaagca acaccggatc ctccgggcga ttgccgttaa
    18301 acagggcggc gcagcccgct gcctgaaagg cgacccactc gtctaccagg ttactggcac
    18361 ccagcaatgc agcagcctgt tcctgtgcgc agaggacgag ccggtggtgc ggatagcaaa
    18421 ggcggagcct gcgtatggcc ggcacggcga ggaggaggtc accgagcgct ccgggatgga
    18481 acaggacgat cgtgcgtgcc atgccgctca agacgattca ggaaggatct tcccatggag
    18541 cgagcgggcc gcccgaagat ctgcgggagt gttgacgtta tagaaggacc gcccagccgg
    18601 atcgatccca accagttcat ccgccgtcac gaggcgcacg cgaagcgacg gatgatcggc
    18661 caaatgctga attttcaaat cgtgctcccg aatgagactt tccatgacgg gcaagcagcg
    18721 tcgatgatag agggcgtgca tcggatgtaa accatttgga agcttggcca tcaccagatc
    18781 ggcttcaccc tggacggtga gaaaataccg gatggtgacc ggatcgagaa acggcatgtc
    18841 acaggctacg gcaaacaccg atggcgtcat cgcttgcttc aaaccggtat agagaccgcc
    18901 caagcttccg cagcccggga tgagatcccg ccagaccggc acgtcagagg cgaggggagg
    18961 actgtcctgg gcaatgacga caacaaccgt ttgaaaaacc gaacgcagca ccgcgagacc
    19021 gtgctcatag agtgtttcct ctccaataga caaaaaacgc ttatcctccc ccattctccg
    19081 acttttccct cccgccagga gcacgcctgt cacatcggca atcatagggt ctccacgccc
    19141 gctcacaaaa aacaagaggg ggtgggtttc cccaccccct ccgtgacgtg tcacataggg
    19201 atcctgacgc gacttacagc gtcttcccct tcttcttatt cgcgcgccgg gtcatcaccc
    19261 atccttccag caataccaca ccgaaggcaa tgattgccgg atagatgtag gtctccttcg
    19321 ggattggggg attcaggaac gtgtagtaga aggccgatac ggccatcttc cgtcctgcat
    19381 caccgttgtg cccgtcccag gcgaagaaca cggtcggcac atattgtccg acttcgaaaa
    19441 aggtatcttc gtcgtaatcc tgatctttct tgttaccgag cgggcgttga atcatcacat
    19501 accaacggcc attcttccat tcgcccttga ggaccttcag gttctcttca taggtgtcac
    19561 gctcttcaaa atctttatcc catcccgtgc ccttgaacgc ccggatggat ccgtcagctt
    19621 cccacttcac gatgtcgacc gggaactgct cgttggtccc gaacagatac cgcggcttga
    19681 tcggagcggg caattccttc catttcacag gagtttcaat agcgatagcg tcgttgtaca
    19741 ccttgtagtt attttgattc gacgcgatgg acccttcctc gccggtcttc ggatcctgct
    19801 ccttgatgtc cagattcact tgcgtcggag cccagggcag cttgccctcg gcgacgctct
    19861 tggtgcggtc atcccactcc aggagataaa cgataaattt gtcgttgtac agcgaacgaa
    19921 cccagatgtc gtcgatccgg ttcacgaagt tgcgtggctt gtgcgtgatc tgtccaccca
    19981 tggccacata ccgtttggag gccttctgcc aggcttcgtt ctccatatcc gtcggaatct
    20041 caccctcgac cgggtcagag ggcaccacga agttgatctt cggcttatcc gtcaacgggt
    20101 cgatgggaag aggctttcca tccgtgtccc gctcgcacag aaaattgacc caatttgcaa
    20161 tgtgccaacg ttcctcaatc gtcgtgttgt ccgcgaatga gggcatcggt gtcccgttca
    20221 caccagtcga gaaggtccgg aaaatgttcc gcacgttata cgggtcttgg cggctgccgc
    20281 ggaagttcca gcacttgtgc caatcggccg gttgaatggc aaaaccccag tcgtctttca
    20341 ggttgaaggc gtttccgtca ccccggcctt cagcaccatg acactccacg cactttttct
    20401 cgacaatgag ctccgatccc ttcttcaggc tttcctccgt cgcagcgatc ggcttgatat
    20461 ctccaagttg gagaaccgtc tgagcttcgg actgcttgtc cgtaaatttc cggtctttga
    20521 ccaactgagt tgtcacgaat gaaagcactt gcaaccttt
//
LOCUS       DASSCQ010000086        11096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1896199, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000086 DASSCQ010000000
VERSION     DASSCQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896199"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            837..1481
                     /locus_tag="VFH05_06040"
     CDS             837..1481
                     /locus_tag="VFH05_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET6677806.1"
                     /translation="MGKVKVAAFTVSLDGFGAGPRQDLQNPLGVRGEELHAWFVDTDV
                     FKKMQGECDGRHGIDNDFAAQSMDNLGAWILGRNMFGPVRGPWKDESWKGWWGDDPPY
                     HTPVFVLTHHARAPLTMEGGTTFYFVTDGIESALKKAKDAAKGKDVRIGGGVDTIRQY
                     LTAGLIDEIHLALSPVFLGEGEHLFIGLNLHALGFTSIKTVASEKATHVLIKKR"
     gene            complement(2193..2534)
                     /locus_tag="VFH05_06045"
     CDS             complement(2193..2534)
                     /locus_tag="VFH05_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677807.1"
                     /translation="MMIVTALRFIAFTRNPRGEMIAKVRDALIANNGWITDFHEYSNV
                     SLCLNFEISMENVQRLCAALRGTGLELTEEPQVMESASDREHDGVPGTLQITFIHHDP
                     DLIIPVPAIPG"
     gene            complement(2760..3635)
                     /locus_tag="VFH05_06050"
     CDS             complement(2760..3635)
                     /locus_tag="VFH05_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET6677808.1"
                     /translation="MRIATWNVNSLKARLEKVMWWLDRAKPDVLLMQETKLTDADAPV
                     QTFQQAGYELVHHGEGRWNGVAIASRCGLGGVIRNFGEPLRSARTSEVGDDEPLAEAR
                     MVAAECGGVRMICVYAPNGRLVGSPFYEAKLAWFDKLARWISKAVTPDTPAVIGGDFN
                     VAPEDVDVWDPQACHGGTHVSERERKAFGRLSSAGFVDAYRLLHQEPGRYTWWDYRAG
                     NFYKNFGMRIDHLLVTTALQNRVVWAEIDREARKGKPVPSDHAPLVIDLDKPGTPFDA
                     GWASAESRIASRVRK"
     gene            complement(3679..4386)
                     /locus_tag="VFH05_06055"
     CDS             complement(3679..4386)
                     /locus_tag="VFH05_06055"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET6677809.1"
                     /translation="MTIQDRLAGLDWKAIEASLWQQGYAKTDSLLTAEQCDALVGLYG
                     KDQLFRSRIDMKRFRFGEGEYKYFAYPLPPLVQILREHIYPRLAVIANAWARALGQPD
                     NFPLSHDKLLGVCRRNGQAKPTPLLLRYAAGDYNCLHQDLYGAVAFPLQLTAFLSRPD
                     LDYSGGEFLLMEQRPRAQSRGEVLAPKQGEIVIFATRHRPVQGSRGYYRVNMRHGVSS
                     VRSGSRFTLGVIFHDAK"
     gene            4523..4900
                     /locus_tag="VFH05_06060"
     CDS             4523..4900
                     /locus_tag="VFH05_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6677810.1"
                     /translation="MKYLLLCCHEEKKVDSMSKSELDAIMEETMSYCEVLKKSGHLLG
                     VEALEPIQTARSVKVQNGKLSVTDGPFAETKEQLGGFFLINARDLNEAIQVASKFPSV
                     RFGTMEVRPVKDFPGSSRPATAS"
     gene            5427..5714
                     /locus_tag="VFH05_06065"
     CDS             5427..5714
                     /locus_tag="VFH05_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677811.1"
                     /translation="MAVSPDRRKLLRAIVASAAAGAVIYLFVMQWALPAVQRQVTHAQ
                     VEWLRDAMKDHLVAVLFAIVAIAAMLGLPVLMVFRWAYGPMVGRKQTTPDE"
     gene            5746..6306
                     /locus_tag="VFH05_06070"
     CDS             5746..6306
                     /locus_tag="VFH05_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET6677812.1"
                     /translation="MKQENHAVSECSVFVGVSLDGFIARPNGDLDWLMGDGGGDSAEY
                     GYDEFIAGIDAIVMGRKTFEKVVMFDKWYYGNKRVVVLSHHSLELAGARARGGVVEQM
                     QGSPAEIVSKLASSGAGRLYVDGGVTIQEFLRAGLIRRLVISRLPVLIGEGIPLFGSL
                     AHDIHLSHIATRTYPGGMVQSEYEAI"
     gene            complement(6303..7688)
                     /locus_tag="VFH05_06075"
     CDS             complement(6303..7688)
                     /locus_tag="VFH05_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6677813.1"
                     /translation="MNETSFQLEDLWASFQGIVPAALSTCAADGTPNITFISQIHYVD
                     SAHVAISFQFFNKTHRNIRENPFACAIALDPRTLHAYRFRLRFDHSESTGPLFEAMSL
                     QLQAVASYSGMTDVFRLLAADVYEVLGVERLEGFTRLPAPKASERSSRLFVDAGLVSL
                     RLIAERLNKALLLDEVLESSLQLLEEQFGFQHSIILLADDQNAKLLTIASHGYPENGV
                     GSEVGMGEGLIGMVARARRTLYLSAMDHGLRYARAVRDRAEAEGRTEAIAREIPLPGL
                     PDAGSQIAVPLVTRGRLVGVLAVESKDPLAFLTRENTLLTTIGSHLATAIDQLSRETD
                     EAPMERTGRQAPRSPSQSGSTRRFTFFPKDDCICVDGDYLIRNIPARILWRLLKQYRD
                     EGRVEFTNRELRMDSWLGLPELKDNLETRLILLRRRLEQKCPDVRLVQRSRGRFALET
                     DRAIDLYEKDS"
     gene            complement(7685..8146)
                     /locus_tag="VFH05_06080"
     CDS             complement(7685..8146)
                     /locus_tag="VFH05_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6677814.1"
                     /translation="MIPDSIVHLLQTGVSVMVGTRDASLMPECTRAWGIQVGADRESV
                     TLFLSEAIGGKTLENLRDNGKIAVTCTRPTDHITCQLKGQVRSIKPATSANHEMSRRW
                     HREFRAELSAIGVPSTLSEAWIIEPTVAVQISVSEVFDQTPGPGAGKKIGQ"
     gene            8285..9136
                     /locus_tag="VFH05_06085"
     CDS             8285..9136
                     /locus_tag="VFH05_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677815.1"
                     /translation="MRNALFGSLVFVLSALMSAPGWASDRNVGEEMRGGNGTVVFVAH
                     DYGFRGPDQIAAGLTTVRILNEGQDLHHIQFVRLLEGKTAADFRAAIAKDPNRLPGWV
                     QYAGGPNAHLPGSLADATVNLTEGDYVVMCWIPDKNGVPHVALGMQKALFVRGGKATK
                     VSTPKPSITIKQVDYQFILSQRITAGLHTIEVMNHGTQPHEVVVVKLVPGATVKDVIA
                     SFEPGAAGPPPGQLVGGITGIEKGERIHFTGEFEPGSYGLICFVPDAASGRPHFLHGM
                     TAEFTVE"
     gene            9263..10306
                     /locus_tag="VFH05_06090"
     CDS             9263..10306
                     /locus_tag="VFH05_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET6677816.1"
                     /translation="MNRISTLVLTIVVSGALVLLGGGFILWLLALPSKGKALAHDAGV
                     AHPALLAHRGASYLAPEESRPAFLMARELRVDYLELDLQRTRDGILVAVHDDTLSRTS
                     NIHEVFPERKGDSVDTFTFAELQQLDIGEWFNRTFPERARKSFHGLRVLQLQEVLEIA
                     EAAFPPIGLCIETKRAHRFPDVERQLVEELTRRGWIQQQGAPGRASLIFQSFDPDSLT
                     RLKALAPQIPRLLLVDEVLLKRLAWEGVLGAAEKVATGIAPWGYGWAFGPEWSSQAGR
                     RYVPTWPWYTRAAHQAGLLVFPWTIDARWEMWMVRLAGADGIFTNRSELAVEVFGRSP
                     SPDIEALWQNIGY"
     gene            10492..>11096
                     /gene="smbP"
                     /locus_tag="VFH05_06095"
     CDS             10492..>11096
                     /gene="smbP"
                     /locus_tag="VFH05_06095"
                     /inference="COORDINATES: protein motif:HMM:NF028095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HET6677817.1"
                     /translation="MQQKRVVLSCAGSFLLVLASASYAADTPAEGTVNPTPEVAANPS
                     ADAVATPAPETAVSPPAEAASNPQPQVAAEPPSGTRMNSDATEQSPQMEKTAMGTSMA
                     VVNDPGRVYLIRENPFFGLHIDKALGHAREAEIAGSLGHTAELLEHAQMSLMRAKEAQ
                     RAGNVPGLNEGIANLRKALKLPLWLTQGSASPSTAQDVDPQ"
BASE COUNT         2422 a         3019 c         3278 g         2377 t
ORIGIN      
        1 tggttccgcg gacgcgcggc aattcgcgaa tcggcaagcc cagccggcgt cgaatggatg
       61 gccggggctg tcgatgtcga tgatcagcgg ggcgtggtcc gaggcacaag ccttccgtgc
      121 ctcgcgaccg atctaggaga aagatggaag aactacttct tattgagtct gtgaggcggg
      181 caggcggcct atttcttgaa gagatcgttc aaggcgcgac gcgggtcgtc agggcgccct
      241 gatgccgacg acgtttctcg ctctacggtg gcgcggagag aaaagaacgt gcagtcattg
      301 cgagcgcgct tgtcggcgat gcgttccgga atagcctggc tgcactcaaa ccgctggcct
      361 ggggcaaagt gggtgcactg ctggcaggca tgtagttctg ctcggcattt gggacactgg
      421 ccaagcccat cagtcaatga ggggagaagt gtgccgcagt cagcgcagcg gaagaccgtt
      481 cggcttggaa gcatcccggg ctgacgcggt cccgacgatc ctgcgggccg tcctccactc
      541 tccttttcgg aatcgcggta tccgtgctgg ccatattttc tgtccgtcat gcggttgccc
      601 tcacttctga tgttagtcta ctccaaaggg caatgtgttt cacttagatg gccgggccgg
      661 tcgaggtcga tgaccacccg cgcgtgatcc gaggggagag gttttcggcg tccgattctt
      721 gcaaatcatc tcgccatcgg catagccttc atctcgccga tcatttcctc taaatctcta
      781 catggacgat cgcatcacga ttcagcaggt tcgggaagag atacatggag gtctgcatgg
      841 gtaaggtgaa ggttgcggcg tttacggtgt cgctcgatgg gtttggtgcg ggccccagac
      901 aggatctgca gaatccgcta ggcgttcgtg gcgaggagct tcatgcctgg tttgtggaca
      961 cggacgtctt taagaagatg caaggtgaat gcgatgggag acatggcatc gataacgatt
     1021 ttgctgcgca gtcgatggac aacctaggcg cctggatctt gggtcgcaat atgttcgggc
     1081 cggtgcgcgg tccgtggaag gatgagtcct ggaagggctg gtggggcgac gatccgccgt
     1141 accatacgcc ggtgtttgtc ctcacgcatc atgcgcgcgc gccgcttacg atggaaggcg
     1201 gaacaacgtt ttactttgtc accgacggca tcgagtcggc cttaaagaag gcgaaagacg
     1261 cggccaaagg caaagatgtt cggattggtg gtggggttga cacgattcgt cagtacctga
     1321 ccgccggcct catcgatgag atccatctgg ctctgtcgcc ggtcttcttg ggagaagggg
     1381 agcacctctt tatcgggctg aatctccatg ccctcggctt cacatccatc aaaaccgttg
     1441 ccagcgaaaa ggcaacacat gtcctgatta aaaagagata aacgctccct acattggtga
     1501 cataacgccg cggcgaatga caaggaccca cgtgctcctg ttgtgaaagc cggcgaagcc
     1561 cagccccgtc gatgaccggg gcagcgccta atcagagagt cgttttccta ttccacgtct
     1621 cgcgattgat gccgcttggt acgtacgata gtttgatcct ttatccaaca ctgaatggga
     1681 gagccagtcg tggtgaagaa ttcggaaatg cagtacctgc accgctgtgt cgaactggcc
     1741 gcagaggctc tggaggccgg ggacgatcca ttcggatctg tgctcgtggc agcaaacggg
     1801 gaggtccttt ttgaggatcg caaccgggta ggctctggtg atcgaactcg gcatcccgag
     1861 ttcgagattg cgcggtgggc ggcggcgaac atgactcccg aggcgagagc cgcgacgacc
     1921 gtttacacct cgtgtgagca ttgcccgatg tgtgccgcag cgcatggatg ggtcgggcta
     1981 ggtcgtatcg tgtacataag ttccgccgag caattgacca catggctaac cgaattgggc
     2041 cttccagccg cgcccgtccg accactgcca atccgtgaaa tagttcctca tctactagta
     2101 gacggtccag ttcatgatct tgctgaacag gtgcacgcct tacaccgccg ttgccacgcc
     2161 ggtgcgtgat cgcactgcct acatgtcgac atctatcccg ggatagcagg aactgggatg
     2221 atcaggtcgg gatcatgatg aatgaacgtg atttgtaaag tgcctggaac gccatcgtgt
     2281 tcccgatctg aagccgattc cataacttgt ggctcttccg ttaattcaag cccggtcccg
     2341 cgaagggcgg cacaaagccg ttgaacattt tccatagata tttcgaagtt caagcatagg
     2401 ctcacattcg agtactcatg aaagtcggtg atccatccat tattcgctat caaagcgtcg
     2461 cgaaccttag cgatcatttc ccctcgcggg tttcgtgtaa aagcgatgaa tcgaagggca
     2521 gtcacgatca tcatgctctt acgagattac atcctcaatg atagccgttt cgaagcgcaa
     2581 tcttagaaaa gatttctgat acaccattac agacacacgc gagttgatcg cagaacggct
     2641 gggccattgg aagtcgatca cgatcgcctg aggttagcgc acgcgaggcc aagagaaaag
     2701 agggccggtg gcattgtcta gcccaaggct aagatcaacg tctcaccggt ggctcatcac
     2761 tacttccgca cgcgtgacgc gattcgcgat tccgcggaag cccagccggc atcgaagggg
     2821 gtgccgggtt tgtcgaggtc gatgaccagc ggggcgtgat cagatgggac aggttttcct
     2881 ttccgcgcct cgcgatcgat ctcggcccaa accacgcggt tttgtaaggc ggtggtcacc
     2941 aataagtgat cgatgcgcat gccgaagttc ttataaaaat tgccggcgcg gtagtcccac
     3001 cacgtgtatc gccccggttc ctggtggagt aatcggtacg catcgacgaa tcccgcgctc
     3061 gaaagccgtc cgaaagcctt ccgctcacgc tcggacacgt gcgtgccgcc atgacaggcc
     3121 tgggggtccc acacatcgac atcttcgggc gcgacattga aatcaccgcc gatgacggct
     3181 ggtgtatccg gagtaacggc cttactgatc cagcgagcca gcttgtcgaa ccacgcgagc
     3241 ttcgcttcat aaaacggtga gcccacgagc cggccgttcg gcgcgtatac acagatcatg
     3301 cgcacgccac cgcattcggc agcgaccatc cttgcctcag ccagcggttc atcgtcgcca
     3361 acttcagaag tccgcgccga ccgcaatggt tcgccaaaat tcctgatcac cccgccaaga
     3421 ccgcaccggc tcgcaatggc cacgccgttc caccgaccct caccatggtg caccagctcg
     3481 tagccggctt gctggaacgt ttggacaggc gcatccgcgt ctgtcagctt ggtttcctgc
     3541 atgagcagca cgtccggctt cgcccggtcc agccaccaca tgaccttttc caggcgagcc
     3601 ttcagagagt tgacgttcca ggtagcgatc cgcatagttg gcaattatcc cttgtgggcc
     3661 cgggtatcag ggcggtcatc actttgcatc gtggaagatc acgccgagcg taaagcgtga
     3721 accggagcga accgagctta cgccgtgccg catgttaacc cggtaatagc ctcggcttcc
     3781 ttgcacagga cggtgccgcg tcgcaaagat cacgatttcc ccctgcttcg gggccaagac
     3841 ctcaccgcgt gattgtgccc gcggtcgttg ttccatgagc agaaactcac caccggaata
     3901 atcgaggtca ggacgactga ggaaggcggt gagctgcagc ggaaacgcga ccgctccata
     3961 cagatcttga tgtaagcagt tataatcccc agccgcgtaa cgaagtaaca gcggtgtggg
     4021 cttcgcctgt ccgttccgac gacagacgcc gagcagttta tcgtggctca gtggaaaatt
     4081 atccggttgg cccaaggccc ttgcccaggc attggcgatc accgcgaggc ggggatagat
     4141 gtgttcccgc aggatctgga ccagcggcgg taatggatag gcgaagtatt tgtactcccc
     4201 ctcgccgaac cgaaagcgct tcatgtcgat tcgacttcgg aacaattggt ccttgccgta
     4261 caggccgacg agagcatcac actgttccgc ggtaagcaat gaatcagttt tggcataacc
     4321 ctgttgccat aacgatgctt caatcgcttt ccaatctaga ccggcaagtc tgtcttggat
     4381 ggtcactgat cttcgctccc gtatttccgg ggaccctcat aactcatgga tttattatgc
     4441 acggactcga tgtcgatttt ggtcattctg agcgactatc tgtcaagcac agcctgtaca
     4501 gtcaaattgg agggtcgaag ccatgaaata tctgcttctg tgttgccacg aagagaagaa
     4561 agtggactcc atgtccaaga gcgagctcga tgccattatg gaagagacca tgtcgtactg
     4621 cgaggtgctg aagaagagcg gtcacctcct cggcgtggag gcactggagc ccatccagac
     4681 agccaggagt gtgaaagtcc aaaacgggaa gttgtccgtg accgacggtc cctttgccga
     4741 aacgaaggag cagctcggag ggttcttctt gatcaatgcc cgcgacttga acgaggcgat
     4801 ccaggtggcc tcgaagtttc catcggtgcg gttcggaacc atggaagtgc gtccggtcaa
     4861 ggattttcca ggcagcagta ggcccgcaac ggcatcgtaa gggagcccca ttgtccggct
     4921 tgttgttcgt gaaaactcat ggaatatcta tgacaacaat aatacacacg tccttcaccg
     4981 gtggatgcgc ctgtggcgcg attcgctatg aaagcacaac ccaacccatg ctgatgcttc
     5041 actgccactg tcgcgattgc cagcgatcga gcggaggacc gttctcgtct ttcactatcg
     5101 tgacgaaaga cgctttcagc cttctgcaag gctcgttgcg ttttcatgac agtcctagcg
     5161 aggctggcgg caagacccat cgaggatttt gtcaaaactg cggttcgccg attgtggtcg
     5221 cgaccgactc acaacctcag accgtcgcaa ttagaacggc cagcttcgat gatccgagtt
     5281 ggttcaaaca acaaatggat gtctggatat ccgacgcgca cccatgggat cagatgaatt
     5341 ccgcaatggt aaagttcgaa aagtatcctc aatcatgatc ctacgctcca ttgagggcgt
     5401 cgtgaagagc cgctagaatc gggagcatgg cggtaagtcc ggatcgtcgg aaactactgc
     5461 gagccatcgt ggccagtgca gcggcagggg cagtaatcta tctgttcgtc atgcagtggg
     5521 cgctgccggc cgtgcaaaga caagtcacac acgcacaagt cgagtggctt cgggacgcca
     5581 tgaaggacca cctcgtggcg gtgctctttg ccattgtggc gatcgctgca atgttgggtc
     5641 tgccagtgct tatggttttc cgctgggcgt atggtcccat ggtcgggagg aaacagacta
     5701 cacctgatga gtgacattgc ggtagcgaca gtcgggggac gggatatgaa acaagaaaat
     5761 catgccgtgt cggagtgctc agtctttgtc ggcgtgagcc tggatggatt catcgcgcgg
     5821 ccgaatggcg atcttgactg gctgatgggc gatggcggcg gtgacagcgc agagtacggc
     5881 tatgacgaat tcattgccgg catcgatgcg atcgtcatgg ggagaaaaac gttcgagaaa
     5941 gttgtaatgt tcgataagtg gtattacggc aacaagcgag tcgtcgtctt gagccatcat
     6001 tcgcttgagt tagccggcgc gcgtgcacgg ggaggcgtcg ttgagcagat gcaggggtct
     6061 ccggcggaaa tcgtgtccaa gctggcttcg tccggcgcgg gccgtctgta tgtggacggc
     6121 ggggtcacca ttcaagaatt tctacgggca ggactcatac ggcgtttggt catcagccgt
     6181 cttcccgtgc tcatcggaga aggcattcca ctgttcggat ccctcgcgca cgatatccat
     6241 ctcagtcata tcgccacacg tacctatccc ggaggcatgg tccaaagtga atacgaggcc
     6301 atctaactgt ccttttcgta taggtcgatt gccctatcgg tttccagcgc gaagcggcct
     6361 cggcttcgct ggacaagtct cacgtcgggg catttctgct caagccgtcg gcgaagcaaa
     6421 atcaatcgcg tttcgaggtt gtcttttaac tcggggagcc ccagccagga atccattcgt
     6481 agctctcggt tcgtgaattc cacacggccc tcgtctcgat actgcttcaa taaacgccag
     6541 agaatccttg ccggaatgtt acgaatcaga tagtccccat ccacgcagat gcagtcgtcc
     6601 ttcggaaaaa aggtaaaccg gcgggttgac cccgactgtg agggagaacg tggggcttgc
     6661 cttccggtcc gttccatcgg tgcctcgtcc gtctcgcggc tcagttgatc gatggccgtg
     6721 gcgaggtgcg atcctatggt cgtgagcaag gtattctcgc gtgtgagaaa ggcgagcgga
     6781 tctttactct cgacagccag gacaccgacc aaacggccac gggtgaccaa tggcacggcg
     6841 atctggctgc cggcgtccgg cagaccggga agcggaattt cacgagcgat agcttccgtc
     6901 cttccctcgg cttcggcgcg gtcccttact gcgcgcgcat agcgcaggcc gtggtccatc
     6961 gcggacagat aaagagtccg acgtgcacgc gcgaccatgc cgatcagtcc ttctcccatt
     7021 cctacttcgg atccaactcc gttctcggga tagccatggc tggcaatggt caatagcttc
     7081 gcattctggt catcggcgag cagaatgatc gaatgctgaa atccgaactg ctcttcgaga
     7141 agctgcaacg aagattcgag gacctcatcg aggagcagcg ctttattcag acgctcggca
     7201 ataaggcgca acgacaccag gcctgcatcg acaaagagcc gcgatgaacg ctcggacgct
     7261 ttgggtgcgg gcagacgtgt gaaaccctcc aaccgttcga cgccgagcac ttcatacaca
     7321 tcggcggcga gcagacgaaa gacgtcagtc atcccggaat aggaagccac ggcctgaagt
     7381 tgaagcgaca tcgcttcaaa caatggcccg gtcgattcag aatggtcgaa gcggaggcga
     7441 aagcgatagg catggagcgt gcgtggatcg agcgcgatgg cgcacgcgaa cggattctcg
     7501 cgaatattgc gatgggtctt attaaagaac tgaaacgaga tggcgacatg agccgaatcc
     7561 acgtaatgga tttggctgat aaaggtaatg ttcggtgtac cgtcggccgc gcatgtgctc
     7621 aaagccgcgg gaacgatgcc ttgaaaagat gcccacaaat cctccagttg aaacgacgtc
     7681 tcgttcattg cccgatcttc ttgcccgcac cggggccggg cgtttgatcg aagacctcac
     7741 tgacggagat ttgcacggcg acggttggtt caatgatcca ggcttcactc agtgtcgagg
     7801 gcacgccgat ggcgctcagt tccgctctga actcgcggtg ccatcgccgg ctcatctcgt
     7861 gattggcgga ggtagccggc ttgatgctcc ggacctggcc tttgagttga caggtaatgt
     7921 ggtcggttgg ccgagtgcag gtgaccgcga tcttgccgtt gtcacgcagg ttttcgagcg
     7981 tcttgccacc gattgcttca gacagaaaaa gcgtcacgga ttcgcggtcg gcaccaacct
     8041 gaatgcccca tgcacgcgtg cattccggca tgagcgacgc gtcccttgtg ccgaccatga
     8101 ccgacacacc tgtctgcaac agatgaacga tcgagtctgg aatcatgaac actgggccct
     8161 taggaaagat ttagggaaac cttagcatag ttttaggaac catgtcgctg accctcgtgt
     8221 taggttcacc ttcgcaaaca atgagcgcgt tccaatcgaa cgcgcgaaac acaggaggga
     8281 tcacatgcga aacgcgttat tcggttctct ggtgttcgtg ctgagtgcgc tgatgtcggc
     8341 gccgggttgg gcgagtgacc ggaatgttgg tgaggagatg cgcggcggca atgggacagt
     8401 ggtctttgta gcccacgact atgggttccg tggtccggac cagattgcag ccggtctcac
     8461 gaccgtgcgg atcctgaatg aaggacaaga tcttcaccat atccaatttg tgcgattgct
     8521 ggaaggcaaa acggctgcgg atttccgtgc ggctattgcc aaggatccaa accgactgcc
     8581 cggatgggtc caatacgccg gaggaccaaa cgcgcatctg ccaggcagcc tggccgacgc
     8641 gaccgtcaat ctgaccgaag gcgactacgt ggtgatgtgc tggattccag acaagaatgg
     8701 cgttccacac gtggcgctgg gaatgcagaa ggcgctgttc gtgcgaggcg gaaaggccac
     8761 gaaggtctca acgccaaaac cgtcaatcac cattaagcaa gtcgactatc aatttatcct
     8821 ttcacagcgc attacagccg gacttcacac gatcgaagtc atgaatcatg gcacccaacc
     8881 ccatgaggtc gtggtggtga agctggttcc cggcgcgaca gtgaaagatg tgatcgcctc
     8941 gtttgagccg ggcgccgcgg gtcctccgcc cggccaattg gtcggtggaa taacggggat
     9001 cgaaaaggga gagcgcatcc acttcaccgg cgaatttgag ccaggcagct atggattgat
     9061 ctgctttgtc ccggatgcag cgagcggccg gccgcacttc cttcacggca tgacggccga
     9121 gttcactgtt gagtaaggat tccgcgagag caccgcaggg gtgggtcgat acaagagaga
     9181 aacacaccgg tcaggggcta gaccttgacc ggtgtgttct tttataaccc cgtacagcat
     9241 cgggccggag aagtctcatt acatgaaccg catatccaca ctggtcctca ccattgtggt
     9301 gagcggtgcc ttggtcttgc tcggcggggg attcatcttg tggcttctgg ccttgccgtc
     9361 caaaggcaag gcattggcac atgacgcggg tgtggcgcat ccggcgcttc ttgctcatcg
     9421 aggcgcctcc taccttgcgc ctgaagaaag ccgacccgcg ttcttgatgg cgcgagaact
     9481 cagagtagac tacctcgaac tagatctaca acgcacgcgg gatggcatat tggttgcggt
     9541 gcatgatgat accctctctc ggacatcgaa tattcatgag gtgtttcctg agcgcaaagg
     9601 cgacagcgtc gatacgttca cgtttgccga actccagcaa ttggatatag gtgaatggtt
     9661 taatcgaaca tttcccgagc gggctcgcaa gagtttccat ggcctacggg tgctacagct
     9721 tcaagaagtc ttggagatcg ccgaggcggc cttccctccg atcggtcttt gcattgagac
     9781 gaaacgagcg catcgatttc ccgatgtcga acgccaactt gtcgaagagt tgacaagacg
     9841 cggatggatt cagcaacagg gcgcgcccgg tcgcgcgtct ctgatctttc agtcgtttga
     9901 tcccgacagt ctgacgcgct tgaaagcgtt ggcgccacaa attccccggc tccttctcgt
     9961 ggacgaggta ctgctgaaac gactggcgtg ggagggagtg ctgggagccg ctgagaaagt
    10021 tgccacgggt attgcaccgt ggggatacgg ctgggcattt gggcccgagt ggtcaagcca
    10081 ggcggggaga cgctatgtgc cgacctggcc ctggtatacg agggcggcac atcaggcggg
    10141 actcctcgtc tttccttgga cgattgatgc ccgctgggaa atgtggatgg taagactggc
    10201 aggagccgat ggaatcttta cgaatcgcag cgaacttgcc gtcgaggtgt ttggtcgctc
    10261 gccgagccct gatatcgaag ccttgtggca gaatatcggg tactgaatga acggcgaagc
    10321 gttggactgt atcgtaaaac aagccagcga caaggcaccc agactgatgg tccgcttagt
    10381 aaaattactg gccgctaggg gtatgcccag tattgccttg ggtctaatta caacagactt
    10441 gcactcccgt acggaaaaac cttgtgatgg ataaaccgaa aggaggcggc catgcaacag
    10501 aaacgtgtcg tgctcagttg cgcgggaagt tttctcctgg tgcttgcatc ggcatcgtat
    10561 gccgcggaca cgcctgccga aggcaccgtc aatccgacgc ccgaggtggc ggccaaccct
    10621 tcggctgacg cagtcgccac gcctgcgcca gagacggcgg tcagtccacc ggctgaggca
    10681 gcgtccaatc cgcagcctca agttgcggca gagccaccat ccggtacaag gatgaattct
    10741 gatgctactg aacagagtcc ccaaatggag aaaacggcta tggggacctc aatggctgtg
    10801 gtgaacgatc caggaagagt gtatcttatc agggagaacc ccttcttcgg tctccatatt
    10861 gacaaggctt taggacacgc ccgtgaagct gaaattgcag gcagcctggg acacactgca
    10921 gaattactgg aacatgctca gatgtcgttg atgcgagcca aagaagcgca gcgggcggga
    10981 aatgtgcctg gtttgaatga agggattgcc aatctgagaa aagcgttgaa actccctctc
    11041 tggttgacgc aaggctcggc ttctccttcc acggcccagg acgtagatcc tcagtg
//
LOCUS       DASSCQ010000087        49611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_521053, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000087 DASSCQ010000000
VERSION     DASSCQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 49611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49611
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521053"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFH05_06100"
     CDS             <1..823
                     /locus_tag="VFH05_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6677818.1"
                     /translation="LSSFKLPDKLTFAGQPVPLDNWQVRERIEYEFYQFLEDQGESIV
                     LAKRTGRCFPPAERQLAEAGLPDDLKYMLLVESKCISAAYSKAKASGPWQFIPSTGRR
                     YRLKSDAVRDERRNLEMSTEAAVKYLKYLKDFQQNDWFMAMASYNAGEERIRKLLKDQ
                     KITDYWKMHGPRETMRYVPRIIAAKEIYSQPEKYLGLSKKDLYIPLETETITVNVKET
                     QRALTSIAEEFGTYFLELKLLNPEFKKDFLPHGTYQIKVPRQSCPTRCFKQEKGP"
     gene            952..2238
                     /locus_tag="VFH05_06105"
     CDS             952..2238
                     /locus_tag="VFH05_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET6677819.1"
                     /translation="MLKHALDAVNGGKAVQQAIHKTGDVLTIGSRRYDLRKYRRIVVV
                     GGGKAAASMARAIEPILGRRLESGVVITKYGHTVRTKCIRVVEAGHPVPDRAGLAAAR
                     GILSLASSLTREDLLVVLLSGGASSLMPSPVSTVTLADKQHVTKLLLRCGAGIRDINS
                     VRKHLSNLKGGRLAASTHATILTLILSDVIGDDLSAIASGPTAPDPSTYHDAIDCLRQ
                     YRLWTSVSRSIRAHLRRGSEGQLTETPKPGTRLFQRVHHEIIGNNQAALDAMLSSARA
                     AGIRTVLLPPLIEDASVSGTYLGSLARSILTKQEPLPRPCCLVAGGETTVHVRGAGRG
                     GRAQEFAVAAAKVVAGLPKVYVAGVATDGTDGPTDVAGALVDGKTWQRAGRLGIDLNA
                     ALKQNDTYRALKRLGSHIITGPTGTNVNDLYLLFVL"
     gene            2253..4871
                     /locus_tag="VFH05_06110"
     CDS             2253..4871
                     /locus_tag="VFH05_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET6677820.1"
                     /translation="MVAPLVLPLAQCHDASLAGGKAVGLARVLKAGLKVPPGCCLTTC
                     AYRTALEAVGFSPVERWRRAIQLQGDDRQRELEDCRQMILRADTTYLRHHLRQTLGEG
                     TGGDQRWAVRSSATNEDMVRVRYAGLYRTELGLPWDEIGRGIAAVWISVWDARVIEYM
                     MKSGTAEAPPAMAVIIQPMLDAALAGVAYSVDPVTGRSGVVTINAIRGLGAPLADGKV
                     TPDQYTVETGPDQPARLIRYIPGHQVERLLLGSSGVTTESVRAEERNASALSEQQLAE
                     VTALVNRVEQAFGGPVDVEWAFDHAGLWVLQSRPITALRSAADLTNEDSEWSRANFKE
                     TLPEVPSPMGLSFLEHFMDAYILSHYRRLGCRIPSDVSPVRTLSGRPYLNVSLFYALI
                     GQLGGNPALLVEQMGGEPLQSPVRVQRLDWRSFLKAAFLMWQEMGRVLRWGPTWFAEM
                     KALALLYSPDRVRSLALEEAEARLDELARWLPSREVTFGIAAGVGQCLQAFSRLLPGW
                     LGRDWRGLLNAALQGLGTVISAQQILHLAELVELARADPAVSDRLGRGEWERGGYRRS
                     FAGSQFLAGFDRYLGDYGHRGTGESDVMSPRMADQPEALLEVIRIQLAGPPTTPAAIA
                     GRQRAAREEALASIKARCGLRVDRWLIFRWWYRRLCLFFALREANRHHLMWYSAAARN
                     LLLHVGKLMTEQGLFRVPEDIFFLTLQERAAMPPERTKNWGALVKARRAQREQWLTLE
                     VPDTIRSPEPPDLNDHLLEAHLMLSGIPVSSGRVSGPVTFVRSEADWINVRRGDIIVA
                     PVIDPGMAPLFGVAAGLVVEMGGTLSHGAIIAREYGLPTITNVRRVMTQLSEGEIIIV
                     DARQGIVTRQAAASGT"
     gene            5133..6524
                     /gene="rmuC"
                     /locus_tag="VFH05_06115"
     CDS             5133..6524
                     /gene="rmuC"
                     /locus_tag="VFH05_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET6677821.1"
                     /translation="MLENPAFGLGLLGGIVLGGLAVGAWISARLRARFDGELCRALAR
                     AQFAETLAEELRRGEQADQAEVDRLRGELADAMRARAIAETQAAETVKRAEEQQTVLA
                     KARHELAESFQALSGEALKQNNEAFLTLARSTFQTLHAEAKGDLAQRQQAIDELVKPL
                     SESLHRYDEQLRHLEQSRQAAYGGLDQHLKLLAETQQRLQQETGNLVNALKAPAVRGR
                     WGEITLKRVAELAGMVAHCDFFEQESVTSSDGRLRPDMVVQLPGGRQIIVDAKTVLGG
                     YLEAHEAADEERRQEGLRRHAAQVRLRINDLSLKAYWSQFDQAPEFVVLFLPGEQFLG
                     AALEQDPRLIEDGFAQGVVLATPTTLMALLRAVAYGWRQERLTAHAEEAGRLGKDLYE
                     RMALLAEYVNDVGLSLGKSVLAYNKAVGSLESRILPAARRFKDLGVSSDKDISSLSPI
                     EVIPRKALSYEQE"
     gene            6511..6963
                     /locus_tag="VFH05_06120"
     CDS             6511..6963
                     /locus_tag="VFH05_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HET6677822.1"
                     /translation="MSKNEQTAPSTAQSMVEEFHRLFGIVVQQTPTVIDEQTRILRER
                     LIHEEFEELKEAMAKNDLFAVAKELADLLYVVYGTAVSYGIDMEPVFREVHRSNMSKV
                     GGYKREDGKWVKPATYSPAMIEPILIEQQAQPGRKRTSQSLAETKSGA"
     gene            6960..7469
                     /locus_tag="VFH05_06125"
     CDS             6960..7469
                     /locus_tag="VFH05_06125"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6677823.1"
                     /translation="MKTLHCPNCGTPFVRVAPQSGMVERVLHHLKVVPFRCQLCMNKF
                     HAFSREATRSVHASDRRQYKRLPTSIQAQFLADNRIRSTNRITDISMDGCTLQTTGLP
                     RGTFLGLVLKPDQEDEAIRIETAMVCSVRPTSVGLRFLEIPLPDQRRLSQVVLGLLVS
                     QGHHPVSNA"
     gene            complement(7560..8453)
                     /gene="panC"
                     /locus_tag="VFH05_06130"
     CDS             complement(7560..8453)
                     /gene="panC"
                     /locus_tag="VFH05_06130"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET6677824.1"
                     /translation="MKTIRSPSAMTTWSDQLHREGVVIGLVPTMGALHDGHRALIRTG
                     RRQCDALVVSIFVNPTQFAPTEDLASYPRPIEQDRELCRTEGVDVCFEPAAGAMYPKG
                     FQTIVTVPAIARRWEGQSRPHHFAGVATVVTKLFNVVRPGLAFFGQKDYQQCGVVRQL
                     VKDLNFPIRIVVCPTMREQDGLAMSSRNVFLSTEDRRIAPIFFHALQAGRQAIENGLS
                     KAASVEALMRKVIGGVPAIRIDYLAVCDPTTLEPLINIDRQAVILGAVRLGSVRLIDN
                     VHARSTHGSRHHRQSLVARED"
     gene            complement(8450..9007)
                     /gene="folK"
                     /locus_tag="VFH05_06135"
     CDS             complement(8450..9007)
                     /gene="folK"
                     /locus_tag="VFH05_06135"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET6677825.1"
                     /translation="MDCETAYIGFGSNVGDRIDFCDRAVTLLSLLPHSQVTGVSLLYE
                     TEPVKDGANPGAGWFLNGVVQLETAITPESLLSMLREIERSLGRDDDNRSGPRTIDLD
                     LLFYGQRVIDQPGLKVPHPRLHMRRFVLMPLSELAPLFVHPTRLRSVNQMLSETTDPS
                     EVRLLFPQPSTRYGSRQACSPRMDS"
     gene            complement(9015..10190)
                     /locus_tag="VFH05_06140"
     CDS             complement(9015..10190)
                     /locus_tag="VFH05_06140"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HET6677826.1"
                     /translation="MVGFPIEVATRIKTLPPYLFAAIDKMKQAAIARGVDIINLGIGD
                     PDLPTPQPIVESLATAAKNPKHHQYPSYEGMLSFRQAVAEWYRRRFGVKLDPADEVLT
                     LIGSKEGIGHIHLAFVNPGDVVLVPSPGYPVYPVGTSFSGGVSHLMPLTKANGFLPDL
                     QAIPKDVAKQAKLMWLNSPNNPTSVVMTKDYFKRVVEFAHQHQVIVCHDAAYSEIFYD
                     GKRPASFMEVEGAKEVGVEFHSLSKTYNMTGWRLGFVVGNKDVLAGLGKVKSNLDSGV
                     FEAVQAAGITALGLDEAVTDAIRRIYQERRDTLVPGLKAVGLDVDTPPAAFYVWVAVP
                     KGYNSASFTEHLLEKAGIVTTPGNGFGAPGEGYVRMTVCTSKERLSEAVERIKKTGW"
     gene            10412..10798
                     /locus_tag="VFH05_06145"
     CDS             10412..10798
                     /locus_tag="VFH05_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677827.1"
                     /translation="MRHRIVLTPIWCAICMMSAAFTGCVAESKDAAKRDVAGPYQTWD
                     EVINRWVGGQVSDLYVELGPPNLHPHQREDGTIEMIWDFAIDRMPGQADAFDLLPLYG
                     GNVNCQIHFFADLTGKVLKGERVGCE"
     gene            10893..11732
                     /locus_tag="VFH05_06150"
     CDS             10893..11732
                     /locus_tag="VFH05_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677828.1"
                     /translation="MTMQFTIKDVEDLRWLLGHTGGFRGGYVTDVQMAKRRLFDEGSG
                     REVLADTLVTVVVRYHLRQMERVAKLTMKGVTDFSIFEQEGADCSSLGVIHAECTAGK
                     LRFWFDPQGELYVVCDEAQLEEVTMPSSDDRLREPLARWTFQSQTAEWPTVEWLLEEL
                     DGAGLPCTWKKAKTPNAGKSVIQWEGTLLPAQGADTIEEAAVHVMLYGPLDGAGFGIL
                     LHVDGSHTSAANRVLSCLSDLIVRRFSGQCLVGNTIIPGGEWLNWKSLEQLRWRRAGK
                     LEE"
     gene            complement(11785..12516)
                     /locus_tag="VFH05_06155"
     CDS             complement(11785..12516)
                     /locus_tag="VFH05_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6677829.1"
                     /translation="MDLHKVERVYTSYAGIYDKIFGKVFHEGRESAIRNLNVQPQERI
                     LEVGVGTGIALPMYPRHCEIVAIDFSEGMLEIAKKRTAEHQMDHVTLHRMDAGAMEFE
                     DSTFDTVVAAYVVTAVPDYRKLVSEMIRVCRPGGRIIMLNHFSNENKMVAAVEKVLSP
                     LTKHLGWRTDLSLDNVLEGTELQVARKQNVNPLRFWALVECVNCKDSDGVAHKKAAAY
                     ANGTTVYTPTPSSLSAGATNEEALA"
     gene            12788..13411
                     /locus_tag="VFH05_06160"
     CDS             12788..13411
                     /locus_tag="VFH05_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677830.1"
                     /translation="MWLAGLFACVLLIGLILGDWLYFVRLTPDAGRYGCGIARSFDRF
                     SVNSLARLQSQFDANGALTLPHGIARYFPDLQQIALRPQYHLFSMSFRTAWPVKGLIH
                     LQLEDQAVHAICIKRIPWSSAIVTLAWFLVVALGTTGFIIAYAAQGGFDSLSGVLMGI
                     GILGIGLLVLAFGVVTVMFSYRLENSRLAKVYEELKSLVNDIQECTQ"
     gene            complement(13444..13788)
                     /locus_tag="VFH05_06165"
     CDS             complement(13444..13788)
                     /locus_tag="VFH05_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677831.1"
                     /translation="MSHSAPSQPDILQSLREEFRFHLETFYAQLKLAPPYESVEKAIR
                     ALTTALHALPKTQQSSIASDPALKWDQFRHAFEISGLSRKHRGIITGLAKDHTSITLP
                     PEYDHFLKFFQS"
     gene            13871..14335
                     /locus_tag="VFH05_06170"
     CDS             13871..14335
                     /locus_tag="VFH05_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677832.1"
                     /translation="MKRIWVAWALFFAVGGCSSPHHHGMMDDKGDRYWQGQQELASLI
                     DQTVKDPQKAQEVKKLAGEIVTELKTGREQEHAYHRQLHVLNNSYTATPEEFMKIIDE
                     GNHQRMRTAAKILSLRFKMKDQMTADEWQALSDRMRAYSGRYQQGGKSKPSD"
     gene            complement(14348..15397)
                     /locus_tag="VFH05_06175"
     CDS             complement(14348..15397)
                     /locus_tag="VFH05_06175"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677833.1"
                     /translation="MWNARLWQFAHPVLIGLALLSIGCQKDSEAIVSIALHPTNSNIL
                     YVATNDAVYKTRDGGQTWEKFPSFSARRVTTVAIDPQLPATVYAGTMGDAVYKSPDGG
                     QHWLPHNVGLKEHVSFVNQFVFHPGLSERIYAATTVGAFYSKDAGREWEERMNGMKEV
                     HIVTSVAIDPRDPSVLYGGTTGGVYRSEDGAMTWKKINNGLIPESELMASMALGVNVI
                     EIDRTNPHVVYAGTTKGLYRTANKGEEWQRIGESIPEPFVSSIVIHPSDSAILYIGGP
                     AGVWKSADGGHTWKATNDGLASLNIRALAMEPKNARTLYAGTNGSGLYRSVDAGERWT
                     AMPLQAAANPREPAT"
     gene            complement(15414..16532)
                     /locus_tag="VFH05_06180"
     CDS             complement(15414..16532)
                     /locus_tag="VFH05_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET6677834.1"
                     /translation="MTSTRRLIFLLGWLAIVLPSIAADTTSFVPAREGYRYAFPRDHG
                     SHDEFRTEWWYYTGNLATNDGRPFGYQLTFFRRGIPLEQVKTLPSRWSITQLYLAHFA
                     VTDLKNGRFHVFEKLSRAGVGKAGAEAERLHVWIDQWRAQSSQAEVGSQTLQASDDDL
                     KIDLTVVPEKAPVIHGKEGVSRKGAESGQASHYYSLTRLATSGTIRLGNESFNVTGTS
                     WMDHEFGSADLGKDLVGWDWFSIQLADHTELMLYRMRRSDGSSDSVSSGTFIDHDGRA
                     QHLTLGEFMLEPLTTWTSPASKARYPSTWRLSIPSKGLSLALVPRMTEQELTTNRSTQ
                     VTYWEGAIEASGTLEGKPIAGRGYMELTGYAERFSKKL"
     gene            16650..17375
                     /locus_tag="VFH05_06185"
     CDS             16650..17375
                     /locus_tag="VFH05_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET6677835.1"
                     /translation="MTILSAIGYRDRMQTDHERDQHEPEPSEKSTPFSIPEHIPVFAL
                     PNVVLFPKTYLPLHIFEPRYRQMVDDAVMGGQCIGIALLKEGWESEYYGNPPVFSTGC
                     VGRLVSVQPLADGRSNILLQGLERFEIIVESYEKPYRQASITLKPRAAEASLDPGVRQ
                     RLVTALEDYLRSRQDAPTWQGWFREDVSDDILVNTLSTYLDCTPLEKQFLLEAATLHQ
                     QARRLSDLIQFLMHDHHGAKGWG"
     gene            17375..18346
                     /locus_tag="VFH05_06190"
     CDS             17375..18346
                     /locus_tag="VFH05_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6677836.1"
                     /translation="MHGAIAIDVSHLSKSYDGHPAVADISFQVYVGEIYGLLGPNGAG
                     KSTTLRTLITLLAPSAGRATIFGLDCVRDADRVRRVIGYVPQERAIDRFLTGREHLEL
                     LAALYHLPKDEAKGRIAELLKLVELEAHADRPAKTYSGGMKRKLDIACGLLPDPKVLF
                     LDEPTLGLDVQSRLRIWDYIRLLKARGLTVVMTTNYLDEADQLCDRLAIIDGGVIKVI
                     GSPVELKIGLGGDIVSLTLKEAERISSLESALRGQPAIKAVRATSKGLDIRVESPEKA
                     LPTILEVANRLGCTVEFVQYNRPRLDDVFMAHTGRRIGEAADPHVVD"
     gene            18367..19146
                     /locus_tag="VFH05_06195"
     CDS             18367..19146
                     /locus_tag="VFH05_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6677837.1"
                     /translation="MHEYWQEISALTMRWVLRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADSQVTQAPNYISFLAAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     TSVILSRFIFVMTITSLQVLVILGVAFLFGVQPATGLLGIATILLIGMMFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLTAMPAWMSVLAKFNPMTWAIDAVR
                     PLILSGWSEALPHVGMVVVVMILFDALCLYGGAKAFRRAMG"
     gene            19206..20069
                     /locus_tag="VFH05_06200"
     CDS             19206..20069
                     /locus_tag="VFH05_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET6677838.1"
                     /translation="MMVIPESQIRALIRLLADEDEKIVQTISGRLIDIGPSAVPLLQE
                     AEIEQPEMAERIASVLEEIRGGKLEDELITLAALPDDRMDLEHGSFLIARYAYPGLDV
                     AAYSRQLDEMAQEVRDRIGPRASGEETVKTLNRYLFTEQGFKGNTKNYYEVENTYLNR
                     VIDRRTGIPISLSIVYLLIGKRLRLPVHGIGMPGHFLVKYESDRYKIFVDCFNGGALL
                     TEKNCQRFLTEAGYGFDERYLQHSPVRAIISRMIKNLLAIYVKVGDPIRQTRLSKFME
                     ILGCEIKEGGL"
     gene            complement(20118..20873)
                     /locus_tag="VFH05_06205"
     CDS             complement(20118..20873)
                     /locus_tag="VFH05_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET6677839.1"
                     /translation="MHLTILGSGTNVHRTRAAAGYLVQTDQTLLLDFGPRTLMNLIKT
                     GVDRHRITHILFSHYHADHFSDFITYLFDEIIYTKFEGGNRPPLTVMGPRGTTRLFKT
                     MFGMLPGFDQAPFPIRYRDLSDRPLRLGSTKIIPGTVLHTPHLHCLGYRIEYRGHALA
                     YSGDSQYCESLVRLCGDVDLAVLDCSFPANRPNPAHLHAGQCGQVAQEAGSGKLVLSH
                     FYPIAERYDVEAQAAEAFAGPIVAARDLLRIKV"
     gene            complement(20876..21334)
                     /locus_tag="VFH05_06210"
     CDS             complement(20876..21334)
                     /locus_tag="VFH05_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6677840.1"
                     /translation="MDLPNLKDDPYLQVLRPLVEAYLAFSRIDSRHIRSLRLTPSQFD
                     VIVTLGDTTGLTCSQLSSQTLVTKGTLTGVLDRLETKGLIKRESVLGDKRRTKIKLTE
                     KGGALFRKVFAAHIAFIRPFFARALSPQEVHTASTLLLRIRDSFQTGGRA"
     gene            21428..22060
                     /locus_tag="VFH05_06215"
     CDS             21428..22060
                     /locus_tag="VFH05_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6677841.1"
                     /translation="MLSRPTGQANLTAIAVLLGTVIAGVWVWKRLSLETQDYILDEAL
                     PMALLAAMGLLVVWGIGRAVHRRKDRLRRRASLLARFSRETTQEQKLETAFALIEANE
                     YRVEGLESVVPALKELFATTLQRAVGDKQHRLRGMAASHIGVLQDLSVVPLLVKALED
                     DHAYVRSCAALALGRLRATETIERLTTVMKDDWDQTVRSRAKEALARMGA"
     gene            complement(22239..23087)
                     /locus_tag="VFH05_06220"
     CDS             complement(22239..23087)
                     /locus_tag="VFH05_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6677842.1"
                     /translation="MIRRIASWPDRVRTCIGHSLSEPFYRFFRLFPNWEIGLDQPEIT
                     HHTHISPPLAGRRAVHLSDLHVDRLQPRHDVVVFTVQELRPDWIFITGDLLNVPEGLP
                     PLFRFLSQLREIAPVFVTLGNHDHYSGVPLSQYAELADRHKITLLINQSTFIPSGSGE
                     LAVVGVDDPSLHRADLRCIPPPANRRYTLLLAHAPNILDMMDEAHAVDLILCGHSHGG
                     QWQIPGVPTFWLPPGCRGRIAGRHESTRHRLYVNRGLGWSFLPFRWNCRPEIAVIHWI
                     EREAAP"
     gene            complement(23084..23701)
                     /locus_tag="VFH05_06225"
     CDS             complement(23084..23701)
                     /locus_tag="VFH05_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677843.1"
                     /translation="MLATWNIVAALPLAFSAFSSEGRGVFVADNGVFPTFTHHIVKQV
                     TIDKQAFAEASTCLSWFYKGEKKPPPAAAQGINRRLSVALQPERDCPRHYPGGMEPAR
                     EDFARKQAMLSVSLTIYEFALVADHNDDQQYNAAELQDIFHSLSLAFDAAAPPGAAGA
                     TLTARFDQWYRTRNLDEVMKGMSTLYERGYRVTQHDRAELDRVMK"
     gene            23743..24777
                     /locus_tag="VFH05_06230"
     CDS             23743..24777
                     /locus_tag="VFH05_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HET6677844.1"
                     /translation="MALPYLTATVPGIGGRLRTEPEDFQVDERPLYLPCGEGEHLYIK
                     VTKRLLSTPDLVRRISSAVGVKTKGVGTAGLKDARAVTTQMLSLHAVTPERVARLKLS
                     DQILAIEVLGRHGNRLRPGHHAGNRFRLVIRDVAPHAKETVPTVLAVLLRRGVPNFFG
                     PQRQGKNGENYHIGAALLTDSSTRAQMSRSKRMWYLNSFQSHLFNQILGRRLEHLDCV
                     WVGDWAMKMENGACFLVEDAQQEKARADRFEISPTGVLFGSRVSWATGQAGEIEQAVI
                     TEQGSTPEALIQSAKACGFRGERRSLRVPLGELEWSLDGSILTLSFVLPPGAYATSVL
                     RELMKIPELG"
     gene            24863..26110
                     /locus_tag="VFH05_06235"
     CDS             24863..26110
                     /locus_tag="VFH05_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HET6677845.1"
                     /translation="MSAATQETVVLSGHIIDSLILAKVLDTILMMGGTFDLEQVSIGK
                     TRNEPSRARIVVRAGSVKLLADILRAIQPHGASVEGEVDCQLKTAPGDGVFPEDFYAT
                     THLPTQVRLSGRWIDVDAIEMDLGIVVNKDRTKAVAVPMAGVNVGDEVVTGRTGIRVV
                     PLQRPQERDVFSFMESQVSAERPHGHIIADVAKRMEVLRDGHRGGKSGCKVLVAGGPA
                     IIHAGGREALTWLIDQGFVHVLFCGNALPAHDMEADLYGTSLGYGLSAGRVVPHGHEH
                     HLRTINRIRGIGSIAKAVETGVITHGIMASCVRERVQTVMAGTIRDDGPLPGVITDSV
                     AAQAAMRAAIPGVGLALLIASTLHAVATGNLLPATIPTVCVDVNPAVPTKLADRGSFQ
                     AVGLVMDAASFLRDVARELGWKG"
     gene            26107..26907
                     /locus_tag="VFH05_06240"
     CDS             26107..26907
                     /locus_tag="VFH05_06240"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HET6677846.1"
                     /translation="MSRLLVCPPDYFSIDYEINPWMRRSNVVAPERAIQQWHRLMEVL
                     ERDIGTVLERMKPVAGLPDLVFTANAGVVVGRKAVPSRFRYPERQREEAHYEGWFRAH
                     GYEIITLDPDMFFEGAGDLLGFHDGWFGGYRQRSDIRAFPVLSELFGKEILPLELIDG
                     RFYHLDTCFCPLSGGDLLYYPAAFDRYAQAAIADRIPTVSRLPVPEQEALKFACNAVC
                     VGKHVVLPDGCPETMTWLESRGYLPHPVPLDEFMKSGGSAKCLTLALD"
     gene            complement(26935..27306)
                     /locus_tag="VFH05_06245"
     CDS             complement(26935..27306)
                     /locus_tag="VFH05_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677847.1"
                     /translation="MYIARTPMNVCPSCRREWPEIYRYCTHCGSQMAGASRAIARPSV
                     APESLNLHVLYGMVALLVLAILFPPWETPPAQPPQFLGLHFILSPPTPDAIVSRVVIT
                     IELVTIAIAGLYGAFLFRSGR"
     gene            complement(27348..28058)
                     /locus_tag="VFH05_06250"
     CDS             complement(27348..28058)
                     /locus_tag="VFH05_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6677848.1"
                     /translation="MRTITALIGATLLAISAPAILSLSTAQAETYVAGQLGVTLPSIG
                     KGLSDVDLTGSFIPGSTTSDQALKSSIMYGGKIGHYFRAAPWFGLEAEIYRTTPHIKQ
                     QTVEFRGPNGPVGSAELAGVHMSVLTLAPLNLSFRYHKTRLQPYVAIGPGIFVAKIKD
                     PALSSDNSQSSTKLGLNAQIGLRYYVTRHVTMFAEGKYNHVRFNFPETPPGSSFNLFG
                     FNATYNMVHVAFGVSYHF"
     gene            28379..29398
                     /locus_tag="VFH05_06255"
     CDS             28379..29398
                     /locus_tag="VFH05_06255"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET6677849.1"
                     /translation="MQPIQHISVIGAGAWGTALAKHLAEKGLQTILWAYERDVLESIA
                     SKRENQLFLPGVALPPSLRVTNTLAEALEQCDGLLFVVPSHVARQVLQQLAPLLSRPL
                     PIISATKGVEEDTFKLMTQVVTEVLPPDLHDGLMVLSGPSFAMEVSQGQPTALCLAGT
                     NAALVGSFQAALITSTLRIYADSDVVGVQLGGALKNVMALAAGVVDGLGLGHNTRAAM
                     ITRGLAEMVRLGMAMGADPRTFYGLSGVGDLVLTCTGTLSRNHHVGVRLGQGDTLDAV
                     LAGMQAVAEGVRTSRAAAGLALRHGVEMPIVREVNAVLFEGKSCRKAVSDLMERVAKP
                     EKGHP"
     gene            29395..30162
                     /gene="larB"
                     /locus_tag="VFH05_06260"
     CDS             29395..30162
                     /gene="larB"
                     /locus_tag="VFH05_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET6677850.1"
                     /translation="MNRERLERLLGEVKSGKASIASALKQLRSLPFEDLGFASLDHHR
                     SIRQGFPEVILCEGKTPAQIRGIAGSLLKHHRPFLATRATRDIAAVIKRIDRRAVYHE
                     SARIVAVRDAQRRLHGRVLVITAGTSDVPVAEEARITAEVMGSRVETLYDVGVAGIHR
                     LLGRHQQLTDAHVIVVVAGMDGVLPSVVGGLVECPVVAVPTSRGYGASFNGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINAFGMKQDRSG"
     gene            complement(30184..30732)
                     /locus_tag="VFH05_06265"
     CDS             complement(30184..30732)
                     /locus_tag="VFH05_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677851.1"
                     /translation="MLGTIPATETPPSCFHLTRALLTCTLASCIFLLHPVALSAKSKP
                     ARAPRPEPELKVLELKISPNPYTVSSGSLEFSTLVQLPRELEGATILEVSSLVTSPSK
                     RSIRFLSLRKPLESPAITPINTDRKTIPVVLTWDGMDHTKAPAEAGIYQFELRAKLLS
                     NGEKGPRTLMLSWPKRGTLEVK"
     gene            30987..32189
                     /gene="larC"
                     /locus_tag="VFH05_06270"
     CDS             30987..32189
                     /gene="larC"
                     /locus_tag="VFH05_06270"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET6677852.1"
                     /translation="MSAHLHFDCFSGISGDMTLGALVDAGAPFGELVKRLKRLGVPGF
                     TLRKRRVQRGAISATKVDVVITSGLLRPWPLSRIRKTLSESRLSEKIKQQSRAVFERL
                     AEAESKAHRVAMDHVHFHEVGLVDSFVDIVGGLICCDLLDVGRVTASAVNVGTGTLQS
                     AHGIIPAPGPAVAILAKGVPIYSAGPARELTTPTGLALLRTLTEDFGPMPQMKPVAVG
                     YGAGDGNPEGWPNVLRVFLSRDAGSDGRGRDTVAQIETNLDDVNPQTYEHITERLFDA
                     GALDVALIPVIMKRGRPAIVLTCLAPFTAVDSLLNVLFEETTALGVRVQEVTRHILSR
                     RFTSVKIRGGTVRIKIAAVDHTSSKAAPEFIDCKRIAERTGRPVKTVLEEATLAYAKT
                     RSTAKDRP"
     gene            32186..33190
                     /locus_tag="VFH05_06275"
     CDS             32186..33190
                     /locus_tag="VFH05_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677853.1"
                     /translation="MTLLGYVLLFGTSVIITLGACALFTNAVEWLGKRLNLSEGAVGS
                     VLAAIGTTLPETSIPIVAIFFGATRQEAEVGLGAILGAPFMLSTLVVPLLAGLLLVYA
                     QLGKRGRSFRLNYKDVTVDLSCFALAYTVAMACTFVPSRPLRILVAVGLLGLYGYYVK
                     IKFSDDDAGGEPSDLSPLVFAKNSARPSYALIGLQAVAGLGGLALGAHLFVMAADSTA
                     QLLAISPLILALLIAPLATELPEMSNSFLWLYQKKDRLAVGNVTGAMVFQGTVPVSVG
                     LVGTEWTLAPSALLTMVLAVLAAGSLLLQAVWSGQWRPWLLTIGAILYIGYACYLYGY
                     "
     gene            33326..34312
                     /gene="pdxA"
                     /locus_tag="VFH05_06280"
     CDS             33326..34312
                     /gene="pdxA"
                     /locus_tag="VFH05_06280"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET6677854.1"
                     /translation="MGDPAGIGPEVIAKALAGTALRRICVPLVIGSQEVMQRTVKQLK
                     LKLAVRPVEGHNPIRLSGGHVAVLDPLERPLGPYKLGVAAAETGAASVAFIKTAVRLA
                     QSECIDGMVTGPINKEAMNMAGCHFPGHTELLAELTQTPESGMMIIGGRLKIMFVTTH
                     IAIKELPAVLTQAKIEKGIRLAHLALTTLFGIKKPRIGVAALNPHAGEHGLFGDEEIR
                     LIGPAARAAQAGGILASDPLPADTLFGKTAKGDYDAVVALYHDQGLIPLKMVAFGSCV
                     NLTVGLPIIRTSVDHGTAFDIVGKGIADPGSLIEAVKLAASLARRRMSHARA"
     gene            34328..34660
                     /locus_tag="VFH05_06285"
     CDS             34328..34660
                     /locus_tag="VFH05_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677855.1"
                     /translation="MPLDVNNALLVIQQQLKELDVIYRDTMQMSLNTVAGTERVAKWR
                     VRTVSLLAESVGEKAAQDFARLQPGMVFTNDLVEEFTDLVDCFRVPLNALAKQLSQTA
                     QRPADGGS"
     gene            34661..35470
                     /gene="rsmA"
                     /locus_tag="VFH05_06290"
     CDS             34661..35470
                     /gene="rsmA"
                     /locus_tag="VFH05_06290"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET6677856.1"
                     /translation="MPGPAFPRANKRLGQHFLIDQNIVRKIVAAAELTPDETVLEVGP
                     GRGILTEALASVARRVVAVEIDPQLHRLLQARQSEWKNVEVVCADALSYSLEAVAAGT
                     VVVGNLPYYISTPLLFKFLEHRHRFPRLVLMLQNEVADRLVAKAGTSAYGVLSVMAQY
                     AADISKAFTVSANCFRPRPEVGSAVVVLRTKQEALLSPEEEPAFAAVVKAAFAHRRKT
                     LVNSLRDEGYDPQAVIDAISAVGIVSTVRAETLSLDTFSRLATRLDRPKPS"
     gene            35533..37956
                     /locus_tag="VFH05_06295"
     CDS             35533..37956
                     /locus_tag="VFH05_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET6677857.1"
                     /translation="MGVTSSAKRGETRRAPAPPSHLKREVIGVMLIALSLLTLLSLLS
                     FVPGEPKVVATPAATMTPPTHNLIGSVGAVFSSLLFSLIGGAAYLFPILLGLLGGRCF
                     TQSDLSIRLRNAGASLAALLFLSGWLHLEVTAVPTVSSGMVFRGQAGGLFGQLLAEGL
                     RAYFANTGAHILIISGFLVALLFTAPLSLAQLAVQAPQWGRNAFARVNALMPKRKQDA
                     VVPDAAKPTKTRKPKSLRAVIEEVLPTPEDNPSAEVFPVTPPTVVLPEPREVVEPEPD
                     APLVGKVEDTGDYQLPDADELLTEPSGVLSRITDEELKGQSDILSKALLSFGIEGRVT
                     EVRPGPVVTMYEFEPAPGTKVARIVNLADDLALAMKAISLRIVAPIPGKSVVGIEVPN
                     SQRETVSMKEVVASDAFTRARSKLTLALGKDIFGTPVTADLKTMPHLLVAGATGAGKS
                     VSLNTMLISILLSAKPNEVKLLLIDPKMLEFQTYDGIPHLLRPVITDPKSAARGLSWV
                     VQEMERRYKLLAEAGVRNIDSYNRKVAGAQGVLGDAGTEAKPDQPELPIRFLSEEERL
                     AAGETAVQDGSPGSFMPPPTPPEPLPYIVVMIDELADLMMVAPKDVEDKIARLAQMAR
                     ASGIHLVLATQRPSVDVLTGLIKANFPARIAFQVSSKTDSRTILDANGAEALLGRGDM
                     LYLASGTGRLMRLHGSFVSDDDVRRVVESVKAQALPSYNQELQSLKQEEAKEEEAQDE
                     VYEQAKELVLSTGQASASLIQRRLRVGYPRAARMIEQMEADGLVGAAGRDGRREVIGR
                     RGPVGDAHE"
     gene            37971..38666
                     /locus_tag="VFH05_06300"
     CDS             37971..38666
                     /locus_tag="VFH05_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET6677858.1"
                     /translation="MGCAVLLTIGVSVTAWAQDVTDDQSRKEVREVVKRLQSRYEKTR
                     DLQADFVQKTTIEGFERPMTSSGKVYIKKPGRLRWNYLEPSTEDIYVDRDDVKVYVPE
                     HKQVLVGKLTQMAASKAPLELLQGAAKLDASFEPEPTPGNGRGIGGVRLVTLVPKAHD
                     GDAHRTVQKIVVEVFPKTYFIRSLWLYESSGNVSNFEFSSLQSNIGLGDDLFVPKLPP
                     DVEIVKAPVLGQP"
     gene            38711..39475
                     /locus_tag="VFH05_06305"
     CDS             38711..39475
                     /locus_tag="VFH05_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET6677859.1"
                     /translation="MSTALLMTVAEATAQAVGKLDVDRLHHEYWDQNEFVVIPQFLPR
                     DFVEATLVPQAQGVKGHLNRNYIPGHKKGGSVSYYTVREQAPLFLDLYRSPDFLAFLS
                     RLTEARLMLCPDNDPHSCALYYYTEAGDHIGFHYDTSYYKGARYTILMGLVDKSTQCK
                     LVCELFKDVPDRTPRTLELVTQPGDMVIFNGDKLWHAVTPLGAGEERIALTMEYVTNP
                     EMGRFKRLYSNLKDSFAYFGIRGVFKGTLASSRKSR"
     gene            complement(39523..39918)
                     /locus_tag="VFH05_06310"
     CDS             complement(39523..39918)
                     /locus_tag="VFH05_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677860.1"
                     /translation="MPTTTVSTPSASDILFHRATSVTSTGPVKHKPEAPRMVRLTVNL
                     PSHLVEQMRDAVYWTPGLTLAWLVARAVRASLTELEAMHQGPFAKRTKPLRAGRPRLL
                     GQSMKVQPKIGGDSVDTSHHEAVSLRPVP"
     gene            39942..40229
                     /locus_tag="VFH05_06315"
     CDS             39942..40229
                     /locus_tag="VFH05_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677861.1"
                     /translation="MWGAQHGVVEDGHGKVACRRRLTRDLVGREAGRRCASGWAQSGG
                     TYLLTVGVYRLSRYYLRRISPLLIEGPLVASCGIAQQQPCHIRQLEFHEES"
     gene            complement(40346..40855)
                     /locus_tag="VFH05_06320"
     CDS             complement(40346..40855)
                     /locus_tag="VFH05_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677862.1"
                     /translation="MDAFQSIVTDLNTLIPIVNHWFHLLSAVIWIGGLAFLVMAVTPG
                     LQKAVAREQIKPITDVFYQHYKKVAGILLVILLFTGGINLHYVNQVLTSQTGSGVQHH
                     AKYLTVFFIKLFLVLCLLTLFLYTVIFKSDDDAEEGETYEVIPFQRAALWMGFFIILC
                     AAAMKHLHQ"
     gene            complement(40955..42340)
                     /locus_tag="VFH05_06325"
     CDS             complement(40955..42340)
                     /locus_tag="VFH05_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6677863.1"
                     /translation="MGKSLPIFNGPSLSGALGSLQNQLTQFLTPKPLAEGPYDGRTVD
                     DFKPYLVALNLTKRCNLKCDHCYLDATTKAAGGDDELSTEECFRLIDQIAEVNKGCLL
                     VITGGEPLVRPDILDIARYAVKLGFMVVFGTNGMLIDDQLAKELVTIGVMGVGISIDS
                     LDPKKHNAFRGVPGAWEAAVAGIEASKRNGLQFQVHFSAQPMNYQELPAVVEWAHGLG
                     ARVLNVFFMVCTGRGEELTDITPAQYEEVLGYLVKCQDEYKGMLVRARCAPHFKRLAY
                     ENDPNSPITKATGYMGGGCLAGTNYARVTPNGEVTPCPYMPLSAGNVRENSFVDLWEK
                     SDIFNSFRYPQLKGKCGDCEYSDICGGCRARPYVDHGDWLDEDQWCLYTPKGGEKIKV
                     AFNVEEASPVQWDDASSLRLSRIPYFLRAMVKKGVEKHAREHGIETVTIELMEELRKK
                     RFGNDAPVFKF"
     gene            complement(42427..44514)
                     /locus_tag="VFH05_06330"
     CDS             complement(42427..44514)
                     /locus_tag="VFH05_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6677864.1"
                     /translation="MYKTIYVPVDNSDHSNQAVDVGVHLAKLFGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDYVDKKCNEANLPI
                     ERRSLEGRNWKVLAEDINTNAYDLVIMGALGVGAVKDSVIGSNTERVVRRVRNSDMLI
                     IKNIQPITGGKIVVAVDGSPYSFGGLMTGLQLGKALNIPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEIIDSGLAKIYQSHLDISREIAQAEQTDVKTTLLDG
                     KAFEKIIQYVRKDVPALLIVGRIGVHSDEDMDIGSNTENLLRSAPCNVLVSNRKYVPP
                     IDTQAEYTIAWTEEALRRMEKIPVFARGVAKTAIHRYAIEKGHTIISNTVVDAAVGHI
                     LPKGAMDAMRALGGNLDAAGIDRDKMQADDAVAKDLMGSTLSGMMTQVVEEKPKTSEA
                     TQAYLDRMTQNYFVCDGCGYIGKGETPVKCPVCGAEGNRFKQVDKSIFEAAAKTEGEV
                     ETDLAYDDVPMQWTKDAKEAIRAVPAGFQRRRAKAKIEKSARKLGMTTITLEYAAPMI
                     QEAASEDYQPIFANKGAGTSAAAEAKLAQANGNGTHDVAPGDKDNTGADASPYHWTTE
                     AQARLERAPEGFMRDCTKALIIKHAEKIGTTHISLEVANEGIEQAKGYMEEAMKTGNL
                     KDMIANLTGKGRA"
     gene            complement(44659..46284)
                     /locus_tag="VFH05_06335"
     CDS             complement(44659..46284)
                     /locus_tag="VFH05_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6677865.1"
                     /translation="MNSILYIFLPCKKVYPIGITYLADFVHRRKPEVRQRILDLSLHP
                     VSQRSRVIRDAMTDFQPDLVCFSWRDIQIFSPHEGDSSLEHAFNFYFASNPIKRVAAS
                     FAGLKQLYRYYSHIYTILSYPWLIRKEFPKSQIMIGGGAFTAFADQLIEKLPEGTIGL
                     LGEGEDAILKLIEGRSIEDERYIIKEGKRVKKGRQGSPALLDALTVDLPYLTSIFPQY
                     REYIGESIGVQSKRGCPYDCAFCLYPYIEGKRVRYRPAEMVVRDISQHYHQWGARRFW
                     FTDAQFITGKEAYPQCTEILERIISEKMEIEWSGYIRTSLITPALAKLMVRSGVGDLE
                     VAITSGSQEVLNTLHMGFKLERLYDGCRYLAEAGFRGKVILNYSLNSPKETEESLLQS
                     VESYRVVASILGEDRVFPLMFFLGIQPNTDLEQRLLQEGYLSAGYNPLMLTPTSIRKL
                     LYNPAPLNKFIAKACLRAWERKQGSRDPRRWTGSLSQRSQTDDSYADQSLSRGIEGNS
                     GRDALLSLEEILRSRRPRPSSTQAPESHATSAG"
     gene            complement(46343..47101)
                     /locus_tag="VFH05_06340"
     CDS             complement(46343..47101)
                     /locus_tag="VFH05_06340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6677866.1"
                     /translation="MTEQQAPDVSARAISTWSGSAREQAVQRMFTAIAGVYDLNNTLL
                     SFGLHSRWKTITASYIPAVERGTALDVGAGTADLALLLEPRMGVRGRVIASDLNHAML
                     VQGLKKVDGKGLTGKITCLQANAEHLGFDDDSFDAVTTGFCMRNVGNLARAVSEIRRV
                     IKPGGTFACLEFSRPVFGWLRAVYDWYSFKLLPWVGTKVARDTTGVYEYLPASIRTFP
                     DQERLCQVLREAGFREVFYKNLSGGIVAIHVARK"
     gene            47174..>49611
                     /gene="uvrA"
                     /locus_tag="VFH05_06345"
     CDS             47174..>49611
                     /gene="uvrA"
                     /locus_tag="VFH05_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6677867.1"
                     /translation="MAKTPRASSERPAILEPSRQSRGAVATGGRFECYNPARMGNSIV
                     IKGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQ
                     FLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTVTEIYDYLRLLFARVGRPYCF
                     QCGEEITAQTVQQMVDAIGALPEGSKFQVLAPIVRGRKGEYRKELLEMRRAGYVRARI
                     DGKPVDLGDDLTLDKQKKHTIEVVIDRLVMKPGDALVRRLADSVETSLKLTGGLVGVL
                     TEEGKTLLYSEKLACIRCGVSYPEITPRIFSFNSPHGACPACDGIGYAMPPDATDDQD
                     LTLLEACEACHGARLRAESLSVKIARQSIADVTGRSVRAAAEFFASLTFTERETVIAH
                     RILKEIRERLGFLVNVGLDYLTLDRAAATLSGGEGQRIRLATQIGSGLVGVLYILDEP
                     SIGLHQRDNRRLLQTLLRLRDLGNTVVVVEHDAETMLAADHILDMGPGAGAHGGHVIA
                     QGTPQQIMSDARSLTGHYLRGVQSVSVPPRHRKPKGFLSVVGAHKHNLKNVTAKIPLG
                     LLTCVTGVSGSGKSTLVLEVLFHSLSQLLYHKKPKIDGCMNLHGVLALDKVIDIDQSP
                     IGRTPRSNPATYTGLFGYVRDLYSNLPESRVRGYTPGRYSFNVKGGRCEACQGDGLIK
                     IEMHFLPDVYVTCEVCKGQRYNRETLEILYKDKSIADVLNMTVEEALAFFENIPLVKS
                     KLQTLHDVGLHYVKLGQSATTLSGGEAQRVKLSRELSKRATGRTLYILDEPTTGLHFA
                     DVQRLLDVLDRLVEA"
BASE COUNT        10253 a        13972 c        14849 g        10537 t
ORIGIN      
        1 tctcagttcg ttcaagctgc ccgacaaact gacttttgca ggacagcccg tcccgctcga
       61 caactggcag gtccgggagc ggatcgaata cgaattctac caattcctcg aggatcaggg
      121 cgaaagcatc gttctcgcca aacgcaccgg ccgctgcttt ccgccggcgg agcggcaact
      181 ggctgaggcc ggcttgccgg acgatctcaa gtatatgctg ctggttgaga gcaagtgcat
      241 ctcggccgcc tattccaaag cgaaggcgtc gggtccatgg cagttcattc cctcgaccgg
      301 ccggcggtac cggctcaaga gcgatgccgt gcgggatgag cgccggaatc tggaaatgtc
      361 cacggaagcc gccgtgaagt acctcaagta tctgaaggat tttcagcaaa acgactggtt
      421 catggcgatg gcgtcgtaca acgccggcga agagcggatt cggaagttgc taaaggatca
      481 gaagatcacg gactattgga aaatgcacgg accacgggag acgatgcgct atgtcccgcg
      541 catcatcgcc gcaaaagaga tctattctca gccggaaaag tacctcggat tgagcaaaaa
      601 agatctgtac attcccctcg agaccgaaac gatcaccgtc aacgtcaagg aaactcagcg
      661 ggcgttgacg tcgatcgcgg aagaattcgg cacctacttc ctcgagctga aattgctcaa
      721 tccggaattc aagaaagact ttttgccgca cgggacttac caaatcaagg tgccgcgcca
      781 atcctgcccg acccgctgtt tcaagcaaga gaagggccca taatgttgtg cgcaacatga
      841 aattcccagc ctgatggcct cggtacctcc tccgctgccg acgcctgctc tcgtctgacc
      901 gcccattcca tgcagcttcc tgcttcgcct gtccgtcagc tcctgatccg aatgttgaag
      961 cacgcgctgg acgctgtgaa cggcggtaag gctgtgcaac aagccatcca caaaaccggc
     1021 gacgtgctga cgatcggatc gcgccgctat gacctgcgca agtatcgccg tatcgtggtc
     1081 gtgggaggag gaaaagcggc cgcctccatg gcgcgtgcga tcgagccgat tcttggccgc
     1141 cggctggaga gtggagtcgt catcacgaaa tacggccaca cggtgcgcac caaatgcata
     1201 cgagtggtgg aggccggcca tccggttccc gatcgggccg gtctcgcggc agctcgaggg
     1261 atactgtcgc tggcctcctc gctcacacgc gaagacctgc ttgttgtgtt gttgtcagga
     1321 ggagcatcga gcttgatgcc gtcgcccgtc tcgaccgtga cgcttgcgga caaacagcac
     1381 gtgaccaaat tgctgcttcg gtgcggcgcc ggcatccgcg acatcaacag cgtccgtaag
     1441 cacttatcga atctgaaggg cggtcggctg gcggcatcga ctcatgccac catcctcacg
     1501 ctcatcctat cggatgtgat cggtgatgat ctcagcgcga ttgcttcggg accgaccgct
     1561 cccgatccca gcacctacca cgacgccatc gactgtctcc ggcagtaccg tctgtggacc
     1621 tccgtttcgc ggtcgattcg ggcacatttg aggcgtggga gtgagggaca gctgacagag
     1681 acgcccaaac caggaacccg actgttccag agagtgcatc atgagatcat cggcaacaat
     1741 caggcggcgc tcgatgccat gctatcgagt gcacgtgccg cggggatccg aaccgttctt
     1801 ctccctccgc tcatcgagga cgccagtgtg tccggaactt atctgggttc gctcgcacgc
     1861 agcattctga caaaacaaga accgcttcca agaccgtgct gcctggtggc aggaggcgag
     1921 acaacggtgc acgtcagggg agccggcaga ggcggccgcg cgcaagaatt tgcggtcgca
     1981 gctgccaagg tggtggccgg ccttccgaag gtgtatgtgg ccggcgtggc caccgatggc
     2041 accgatggtc cgacggatgt ggccggagct cttgtcgatg gaaagacgtg gcaacgtgcg
     2101 gggcggttag gaatcgatct caacgccgca ctgaaacaga acgacacgta ccgtgcgttg
     2161 aaacggctcg gctcccacat cattaccggt ccaaccggca ccaatgtcaa tgacctttac
     2221 ctgctctttg tcttgtaggt actatccgac tgatggttgc tccgctcgtg ctgccactcg
     2281 cccaatgcca tgatgcttcg ctcgccggcg gcaaggccgt tgggctcgcg cgcgtgctga
     2341 aggccggact gaaggtgccg ccggggtgct gtctgacgac atgtgcctat cgtaccgcat
     2401 tggaagcggt cgggttttcg ccggttgaac gctggcggcg ggcgattcaa ctccagggtg
     2461 acgaccggca gcgggagctc gaagactgcc ggcagatgat tctgcgcgca gacactacct
     2521 acctccggca ccacttgcgg caaacccttg gggaggggac agggggtgat cagcggtggg
     2581 cagtacgttc gtcggcaacc aacgaagaca tggtccgggt cagatatgcc ggcctgtatc
     2641 gaacggagct cggactcccg tgggatgaga tcggacgggg catcgcagcg gtctggatct
     2701 cggtgtggga tgcgcgagtg atcgaataca tgatgaagag cggaacggcc gaagcgccgc
     2761 cggccatggc ggtgatcatc caaccgatgc tggatgcagc gctcgccgga gtcgcctatt
     2821 cggtcgaccc ggttactggg cggagcggtg tagtgaccat caacgccatt cgcgggttgg
     2881 gagctccctt ggccgacgga aaggttacgc cggatcagta cacggttgag acagggcccg
     2941 atcagcccgc gcggctgatc cggtacattc ccggccatca ggttgagcgg ctattgctcg
     3001 ggagcagcgg tgtcacgact gagtccgttc gtgccgagga gcgcaacgca tccgctctct
     3061 cggagcagca actcgccgag gtgactgcgc tcgtcaaccg agttgaacag gcgttcggcg
     3121 gaccggtcga cgtcgaatgg gcgtttgacc atgcagggct atgggtcctt caatcgcgac
     3181 cgatcacggc gctccgatca gccgctgatc tcaccaatga agatagcgag tggtcgagag
     3241 cgaactttaa ggaaacgctg ccggaggtgc ccagtccgat gggtctttcg ttccttgaac
     3301 acttcatgga tgcctacatc ctctcccact atcgccggct gggttgtcga atcccctccg
     3361 atgtttcgcc ggtccgtacg ctgtcaggtc gcccctatct gaacgtgtct ctgttctacg
     3421 ccttgatcgg tcaactgggc ggcaaccccg ccctgcttgt ggaacagatg ggaggcgaac
     3481 cactgcaatc gcccgtgagg gtgcaacgat tggactggcg gtcattcctc aaagccgctt
     3541 ttctgatgtg gcaggaaatg ggacgagtcc tgcgatgggg tcccacctgg ttcgcagaga
     3601 tgaaggcgct cgctctcctt tatagtcctg accgcgtgcg gagtttagcc ctggaggagg
     3661 ccgaagcccg acttgatgag ttagctcgct ggctgccttc ccgcgaagtg acgtttggaa
     3721 ttgcggcagg agtgggccag tgtttgcaag ccttcagccg gctgctgccc ggttggctgg
     3781 ggagggattg gcgcgggtta ttgaacgccg ccttgcaggg gctgggcacc gtcatcagcg
     3841 ctcaacaaat tctacaccta gccgagttgg tcgaactggc ccgggccgac ccggctgtct
     3901 ctgaccgact cggccgcgga gaatgggaac gaggcggtta ccgtcgaagc ttcgccggga
     3961 gtcaatttct tgccgggttc gaccgctacc tgggagatta tgggcatcgc ggtacagggg
     4021 aatcggacgt catgtctccg cggatggccg atcagcccga agcgctgctc gaggtgatcc
     4081 ggattcagct cgccggcccg ccaaccactc cggcagccat tgcgggacgc caacgggcag
     4141 cgagggaaga ggcattggcg tcgatcaagg ctcgttgcgg ccttcgtgtg gatcgttggc
     4201 tcattttccg ctggtggtat cggcggctat gtcttttctt cgccctgcgc gaagccaatc
     4261 gccaccattt gatgtggtat tccgctgcgg cgcgaaacct gctgcttcat gtcgggaagc
     4321 tcatgaccga acaaggcctg ttccgcgttc cagaggacat cttctttctg acgctgcaag
     4381 aacgcgcggc catgccaccg gagcggacaa agaattgggg agctctggtc aaggcccgcc
     4441 gggcccagcg ggaacaatgg ctgaccttgg aggtaccgga tactattcgg agcccggaac
     4501 cgccggacct gaacgatcat ctcctggagg cccatttgat gctctcgggc attcccgtta
     4561 gttccggacg agtatctggg ccagtcacct ttgtccgatc agaggcggat tggatcaatg
     4621 tgcgacgagg ggacatcatt gtcgcaccgg tcatcgatcc tggcatggcg ccattgttcg
     4681 gtgttgcggc aggattggtc gtggaaatgg gcggcacgct ttcacacggg gcgatcatcg
     4741 cccgcgagta tgggttgccc acgatcacca atgtgcgccg ggtcatgact caactgtccg
     4801 aaggtgaaat tatcatcgtg gacgctcggc aaggtatcgt gacacgtcag gcagccgcgt
     4861 cgggaaccta acagccgtgc cgacaagaaa cgtgttgcgc tcgttggaca tccgcgctcg
     4921 tctcgtctgc aggcaagact ttgtcaacaa ccctaaggat aatttcttga tgcagacttc
     4981 tcgtctgtag ggccgaacgg ctctgagtat tttcctgccc ttgacgtttt tcatgatatt
     5041 acgtaacctc ccgcagctaa tcgttcaatt gcctgtccat acatccatac cggtaccaga
     5101 gtttctctcc gtgcatgaac ggagtccgac ctatgcttga gaatccggcg tttggcctcg
     5161 gcctccttgg ggggattgtg cttggaggac tggctgtggg cgcgtggatt tctgctcgct
     5221 tgcgagccag attcgatggc gaactctgtc gtgcgctggc acgtgcgcaa tttgccgaga
     5281 cgctggccga ggaattgcgg cgtggggagc aggccgacca ggcagaagtc gaccgtctcc
     5341 gcggtgagct cgccgatgcc atgcgagccc gcgcgatcgc tgaaacgcag gcagcggaaa
     5401 ccgtcaaacg tgcagaggaa caacagaccg ttctggcgaa ggcccgtcac gagctggccg
     5461 aatcgtttca agctctctcc ggagaggcac tcaagcagaa taatgaggca tttctcacgc
     5521 tcgccaggag cacgtttcag accttgcatg ccgaagcgaa gggtgatctc gcccaacggc
     5581 aacaggctat cgacgaatta gtcaagcctc tctctgagtc cttgcaccgg tatgacgaac
     5641 agcttcgtca tctcgagcag tcacgccagg ccgcctacgg tggcctcgac cagcatctta
     5701 aattgcttgc agaaacacag cagcggttac agcaggaaac ggggaacctg gtcaatgcgt
     5761 tgaaggcgcc ggccgtccgc ggccgttggg gtgagatcac gctgaagcgc gtcgccgaat
     5821 tggccggcat ggtcgcgcac tgtgactttt ttgagcagga atcggtgacg tcgtccgatg
     5881 ggcggcttcg accggatatg gtcgttcagc tcccgggcgg acgtcagatc atcgtggatg
     5941 cgaaaacggt gttgggcggg tatctcgaag cgcatgaagc cgcggacgaa gagcgccgtc
     6001 aggaaggctt acggcgccat gcagcacaag ttcggttgcg catcaatgat ttgagcctca
     6061 aagcttactg gagccaattc gatcaggcgc cggagttcgt cgtgctgttc ttgccgggcg
     6121 agcaattcct cggagcggca ttagagcagg atccacggct gatcgaagac ggctttgcac
     6181 aaggtgtcgt cctcgccacg cccaccaccc tgatggcgct cctcagagcc gtagcctacg
     6241 gctggcgaca ggagcggttg actgctcacg cggaggaggc cggtcgattg ggaaaggatc
     6301 tctatgaacg catggccctg ctcgccgaat atgtgaacga cgtgggactg tcgctcggca
     6361 agagcgtgct agcctataac aaggcggtcg gctccttgga gagcagaatt cttcccgcgg
     6421 cacgccggtt caaagatttg ggcgtgtcgt ccgacaaaga catttcatcc ttgagtccta
     6481 tagaagtcat tccgcgaaag gccttgtcgt atgagcaaga atgagcaaac agccccatcc
     6541 acggcccaat cgatggtcga agagttccat cgtttgttcg gcatcgtggt ccaacagacg
     6601 ccgacggtca tcgatgagca gacccgtatc ttgcgagaac gcctcatcca tgaggaattt
     6661 gaggaactga aagaggccat ggccaagaac gacttgttcg cggttgcgaa ggaacttgcg
     6721 gatctcttgt atgtcgtcta tgggaccgcc gtatcgtatg gtatcgacat ggagccggtc
     6781 tttcgcgaag tgcaccggtc caatatgagc aaagtcggag gctataaacg cgaggacggt
     6841 aaatgggtca agccggcaac ctactcgccc gctatgatcg aaccgatatt gatagaacaa
     6901 caagcgcaac ccgggaggaa acgaacttcc cagtcactgg cggagacaaa gtcgggagca
     6961 tgaagactct ccattgtccg aattgcggaa cgccgttcgt tcgcgtcgcg cctcaatcgg
     7021 ggatggtgga acgtgtgctg catcacctga aggtggttcc gttccgctgt caactctgca
     7081 tgaacaagtt tcatgccttt agccgcgagg cgacgcgcag tgttcacgcg tcagatcgcc
     7141 ggcagtacaa acggcttccc acctcaattc aggcgcagtt ccttgcagac aatcggatcc
     7201 gttcgacaaa tcggatcacg gacatttcga tggatggctg cactctgcaa acgaccggcc
     7261 tgccgcgggg aacgttcctg ggtctggtgc tcaagcctga ccaggaagac gaggcgatcc
     7321 gaatagaaac ggcgatggtc tgctccgtgc gtccgacgtc tgtagggctg cgctttctcg
     7381 agattccatt gcctgatcag cggcgcctca gccaagtggt cctcggcttg ctcgtgagcc
     7441 aaggccacca tccggtctcc aacgcttagt ctgacgagcc gccagcgacg ttctccctta
     7501 actcaaagac tttcgttccg cagcaccgga cctctcgccg agttcgatac caacatggtt
     7561 cagtcttcgc gagccacaag gctctgccga tggtgtcgtg aaccatgtgt actcctcgca
     7621 tgcacgttgt cgatgaggcg tacggaccca aggcggaccg caccaagaat gaccgcctgg
     7681 cgatcgatgt tgatgagagg ctccagcgtc gttggatcgc aaacagccaa ataatcgatc
     7741 cgaatggcgg gcactcctcc gatcaccttc cgcatcagcg cctccactga cgcggccttg
     7801 ctgaggccgt tctcaatcgc ctggcggccg gcttgtaagg cgtggaaaaa gatcggcgca
     7861 atgcggcgat cttccgttga cagaaacacg ttacgggaac tcatcgccag gccgtcttgc
     7921 tccctcatgg ttgggcagac gacaatgcgg ataggaaaat tcaaatcctt gaccaattgc
     7981 cgcacgaccc cgcactgctg atagtctttt tgtccgaaga acgccaggcc gggccggacg
     8041 acgttgaaga gcttggtgac gacggtcgcc acgccggcaa aatgatgggg gcgggattgc
     8101 ccttcccacc ggcgtgcgat ggccggcacc gtcactatcg tctgaaaacc cttgggatac
     8161 atcgcgccag ctgccggctc gaaacagaca tcgacgcctt ccgtccggca taactctcta
     8221 tcttgctcga ttggcctggg atagctcgcc aaatcttcgg ttggagcaaa ctgtgtcggg
     8281 ttcacaaaaa tactgacgac gagtgcgtca cactgacgtc ttccggtgcg gatcaatgcc
     8341 cgatgcccgt cgtgaagcgc acccatcgtt ggcaccaggc cgatgacgac cccctctcga
     8401 tgcagctgat cgctccatgt cgtcattgcc gaaggcgatc ggatcgtctt catgaatcca
     8461 tgcgggggct gcaggcctgg cgcgaaccgt agcgggtcga cggttgcgga aagaggagac
     8521 ggacttcgga tggatcggtg gtctccgaga gcatctgatt caccgaacga agacgagtag
     8581 gatgtacgaa gagcggagcc aattcgctca agggcatgag cacaaagcgt cgcatgtgga
     8641 gtcgcggatg gggaaccttc aagccgggct ggtcaatgac gcgctgtcca taaaacagca
     8701 gatcaagatc gatcgtccgc ggacccgaac ggttgtcgtc gtcccggcca agggagcgct
     8761 caatttccct caacatgctc aacaggctct ccggcgtgat ggctgtctcc agctgtacga
     8821 cgccattgag gaaccatccg gcgccggggt tggctccgtc tttgaccggc tcagtctcat
     8881 agagcagcga aacgccggtg acctgcgagt gcggcaatag actcaagagc gtcacggcac
     8941 ggtcacaaaa atctatccga tcgccgacat tggagccgaa accaatatag gccgtctcgc
     9001 agtccatctc ggcgttacca ccccgttttt tttatccgct cgaccgcttc actcagccgc
     9061 tctttcgagg tacagactgt cattcgaacg tatccttcgc ccggcgcacc gaatccatta
     9121 ccgggagtgg tcacgattcc ggccttctcc agtaaatgtt ccgtaaacga cgcagagtta
     9181 taacccttcg gaaccgccac ccagacataa aacgcggcgg ggggtgtgtc gacatcaagt
     9241 cccaccgcct tcaatccggg aaccagcgtg tccctccgtt cctggtagat gcgcctgatg
     9301 gcgtcagtga ccgcctcatc aagtcccaac gccgtaatgc ccgccgcttg cacggcttcg
     9361 aatacgcccg agtcgagatt gcttttcacc ttgccgagcc cggccaacac gtccttgttg
     9421 cctacgacga atcccagccg ccacccggtc atattgtatg tctttgacag ggaatgaaat
     9481 tccacgccga cttctttggc accctcgact tccatgaaac tagccggccg tttgccgtca
     9541 tagaaaattt cggagtacgc tgcatcatga cagacgataa cctggtgttg atgcgcaaat
     9601 tcgacgacgc gcttgaaata gtctttggtc attaccaccg aggtgggatt gttcggggaa
     9661 ttgagccaca tgagcttggc ctgttttgcc acgtccttag ggatcgcctg cagatcaggc
     9721 aggaacccgt tggccttcgt gagcggcatg agatgcgaaa cgccgccgct gaaactggtg
     9781 ccgaccggat acacgggata gcccggactt ggcacgagca ccacgtcccc tggattcacg
     9841 aaggccagat ggatgtgtcc gatgccttcc ttcgaaccga tcagggtcag cacttcgtct
     9901 gccggatcca gcttcacacc aaaccggcgc cgataccact cggccaccgc ttgacggaat
     9961 gacagcatcc cctcatatga ggggtactga tggtgtttgg gattttttgc ggctgttgcg
    10021 agactctcga caattggctg gggtgtcggc agatcgggat cgccgatgcc caggttgatg
    10081 atatccactc cgcgggcaat tgcggcctgc ttcatcttat caatggcagc gaacaaatag
    10141 gggggcaatg tcttgatgcg cgtcgcgact tcgatgggaa aaccgaccat gatcgaggaa
    10201 tctcctttcg tcaagaacgc ttgttcgaca ccgagcctct aggctactgc aggaataagg
    10261 cggtaatcaa tgtaagagac aagcggcggt tcttcacgag cgtcttccgc ggcctgagtg
    10321 aagcgaaatc tctgaattga ctgatgatcc gctgtccaat tatgctgcgc ggttccaggg
    10381 ctagcaaggc atctctaccg aaaggttgac catgcggcat cgaatcgttt tgacgccgat
    10441 ctggtgtgcc atctgcatga tgagcgcggc gttcaccggc tgcgtcgcgg aaagcaaaga
    10501 cgccgcgaag cgagatgtgg ccggccccta tcagacgtgg gacgaggtca tcaaccggtg
    10561 ggttggaggg caggtttcgg acctctacgt cgaacttggt ccgcccaatc tccatccgca
    10621 tcaaagagag gacgggacca tcgagatgat ttgggacttt gcgatcgatc gcatgccagg
    10681 tcaagccgat gcatttgatc tccttcctct ttacgggggc aatgtcaact gccagatcca
    10741 ttttttcgcc gatttgacgg gcaaggttct caagggagaa cgtgtgggct gcgagtgaca
    10801 ggctcgcttc attcacggct gtctctgatc aagtggtgag tgcggacgcc cggcgatcgt
    10861 tctggctacc ggcgtcatcc tgcgttacaa tcgtgactat gcaattcacg atcaaagatg
    10921 ttgaagatct ccgttggcta ctgggccaca cgggtggctt ccgcggtgga tatgtgacgg
    10981 acgtgcagat ggcgaaacgt cggctgttcg atgaagggtc cggccgcgag gttctggctg
    11041 atacccttgt gacggtcgtc gtgcggtacc atcttcgtca aatggagcgc gtggcaaaac
    11101 tgaccatgaa aggcgtcacg gatttttcca tttttgagca ggaaggcgcc gactgttcca
    11161 gtttaggcgt gatccatgcc gagtgtacgg ccggcaagct tcggttctgg ttcgatccac
    11221 agggcgagct ctatgtcgtc tgcgacgagg cgcagttgga agaagtgacg atgccgtcgt
    11281 ctgacgaccg tttgcgcgag ccgcttgccc gctggacttt ccaaagccag actgcggaat
    11341 ggccaacagt agaatggctg ctcgaagagc tggacggagc agggttgcca tgcacatgga
    11401 agaaagccaa aacaccgaat gcaggaaagt cggtcatcca atgggaaggt acgctgcttc
    11461 cggctcaagg agcggatacc atcgaagagg ccgccgtcca tgtcatgctg tatggaccgc
    11521 tcgatggagc ggggttcgga atcctgttgc atgtggacgg ctcccacacc tcagccgcca
    11581 accgcgtcct gtcctgcctg tctgatctca tcgtcagacg tttttcgggt cagtgcttgg
    11641 tcggcaatac catcatcccc ggtggagaat ggttgaattg gaagtcgttg gagcagttac
    11701 ggtggaggag ggcagggaag ctcgaagaat aatcagaatg ccgttgccgg gaccgtagaa
    11761 tggagcaaga gagtggttct cacgctaggc gagggcttct tcgttcgttg ctccggcgct
    11821 gaggctgcta ggagtcggag tgtagacggt cgtgccgttc gcgtaggcgg cggctttttt
    11881 gtgcgctacg ccgtcgctgt ccttacaatt cacgcattca accagggccc aaaatcgtag
    11941 cggattcacg ttctgcttcc gggcgacctg caactcggtt ccttccagca cattgtccaa
    12001 ggacaggtcc gtgcgccagc ccagatgctt cgtcagtggc gacagcactt tttccacggc
    12061 ggcaaccatc ttattctcat tgctgaaatg attgagcatg atgatgcggc caccggggcg
    12121 gcagacccga atcatctcgc tcacgagctt gcggtaatcg ggcaccgcgg tcactacata
    12181 tgccgcgacg accgtatcaa atgtgctgtc ctcgaattcc attgcgccgg catccatgcg
    12241 gtgtagtgtc acatggtcca tttggtgctc tgccgtccgc ttttttgcga tctccagcat
    12301 gccttctgaa aaatcgatcg cgacgatttc acaatggcgc gggtacatgg ggagggcaat
    12361 acccgttccg acgccgactt ccaggatgcg ttcttgcggt tggacgttca ggttgcggat
    12421 agcggattca cgcccttcgt gaaagacctt tccaaagatc ttgtcgtaga taccggcgta
    12481 ggacgtgtag acgcgctcga ccttatgcaa atccatatga cctccaaccg cacgtgagac
    12541 ttctgcttct gcccgacagc agggatgaac cgaggatcga ttccaatggg gagaatccgc
    12601 accggcggag cgcctaaatg gagcgaggaa cccgcctaac ctgacacaat cgctacaata
    12661 accaagtcct gatatcgagt caatacaata gatttggacc tagggaaatc ctagagggct
    12721 accgcgtagg cgagtgcggg agggttccca gttgattcga ggcttcggct aagcataggg
    12781 tacgaccatg tggctggctg gcctcttcgc gtgcgtgcta ctcattggat tgattcttgg
    12841 agattggctc tacttcgtac gcttgacccc ggatgccgga cggtatggtt gcggcattgc
    12901 acgcagtttt gaccgattta gcgtgaacag cctcgcacgt cttcagtcgc agttcgacgc
    12961 caacggcgcg ctgacgctgc cgcatggcat tgcccggtat tttccggacc ttcaacagat
    13021 cgcgctgcgc ccgcaatatc acttgttctc gatgagtttc cggaccgcgt ggcccgtcaa
    13081 ggggctcatt cacctgcagt tagaggacca ggcggtccat gccatctgca ttaagcgaat
    13141 tccctggtca tccgccatcg tcaccctggc ctggttcctt gtagtcgctt tgggaacgac
    13201 gggtttcatc atcgcctacg cggcacaagg tgggttcgat tctctgagcg gtgtgctgat
    13261 gggcatcggg attctcggaa taggcttgct cgtattggcc tttggcgtgg tcacggtcat
    13321 gttttcctac cggctggaaa acagccggct ggcaaaggta tacgaggaac tgaaatcctt
    13381 ggtgaatgac attcaggaat gcactcaatg acgccggtgg gtaccgcaca gggaccagcg
    13441 atttcaggac tgaaagaact tcaaaaagtg gtcgtattcg ggggggagag tgatggaggt
    13501 gtggtctttg gccaggcctg tgatgatccc gcgatgtttt ctgctcaacc cagagatttc
    13561 gaacgcgtgg cggaattggt cccatttcaa agcggggtct gaggcaatgc tggactgctg
    13621 tgttttcggc agtgcatgca gagcggtggt caacgcacgg atcgctttct cgacgctttc
    13681 gtagggaggg gccaatttga gctgggcgta gaaggtttcc aaatgaaacc ggaattcctc
    13741 tcggagcgac tgaaggatat ccggctggga tggagcggag tggctcatcg caccatcgat
    13801 gatacgggaa gacgattccc actgacaatg ggcctagagg gagtgatgtg tggtagctaa
    13861 ggaggtccgc atgaaaagaa tctgggtggc atgggcactg ttctttgccg tcggagggtg
    13921 ctcctctccc catcaccatg ggatgatgga tgacaaagga gaccgatatt ggcaagggca
    13981 gcaggagttg gctagcctga ttgaccaaac cgtgaaagat cctcaaaagg ctcaggaggt
    14041 caagaaactc gcgggtgaga ttgtcacaga actgaaaacg ggccgtgaac aagaacacgc
    14101 gtaccaccgt caactgcatg tactgaacaa tagttacacg gccacgccgg aagaattcat
    14161 gaagatcatc gatgagggaa accatcaacg gatgcggact gcggcaaaaa ttttgagcct
    14221 gcgttttaag atgaaggatc agatgaccgc cgacgagtgg caggctcttt ccgatcggat
    14281 gcgggcgtac agcggtcgat accagcaagg aggcaaatca aaaccgagcg attaaagaag
    14341 aaggaggtca cgtcgcaggt tcgcgtgggt tcgcggcggc ttgcagaggc atagccgtcc
    14401 acctttcgcc ggcatcaacc gatcggtaca gcccgcttcc attagttccg gcatagagtg
    14461 tccgggcgtt tttcggctcc atcgccagcg ctcggatgtt taatgaagcg agcccgtcgt
    14521 tggtggcctt ccacgtatga ccaccatcgg cgctcttcca cacccctgcc ggccctccga
    14581 tatacaagat cgcagagtca gaaggatgaa tcacaatgct gctcacgaac ggttctggaa
    14641 tcgattcccc gatgcgctgc cactcttctc ccttgtttgc cgtccggtag agacccttgg
    14701 tggtgccggc atacaccaca tgaggatttg tccggtcgat ttcaatgacg ttgacgccca
    14761 aagccatgga ggccatcaat tcgctctccg gaatgagacc gttgttgatt tttttccagg
    14821 tcatcgctcc gtcctctgac cggtatactc ctccggtcgt ccccccgtac agcacggatg
    14881 gatctcttgg atcaatggcg accgacgtga cgatatggac ttccttcatg ccattcatcc
    14941 gctcttccca ttcacgcccg gcatctttgc tgtagaaagc tcccacggtg gtcgcggcat
    15001 aaatccgttc gctcagcccc ggatggaaaa caaattgatt cacaaaggac acgtgctcct
    15061 ttaatcccac attatgcggc agccaatgct gccctccgtc gggactcttg tagaccgcat
    15121 ctcccatcgt gcctgcgtac accgtggcgg gtagctgcgg atcgatggcc acggtcgtta
    15181 ctcgtctggc gctaaagctg ggaaatttct cccaggtttg gccaccgtcg cgcgttttgt
    15241 acacggcgtc gttggtcgcg acatacagga tattcgaatt cgtgggatgg agagcgatgg
    15301 agacaatcgc ttcgctgtct ttttgacagc cgatgctgag aagcgccaac ccgataagca
    15361 cgggatgggc gaactgccat agacgtgcat tccacatctg acgccttttt tgtctacagt
    15421 ttcttcgaga agcgctccgc ataaccggtc agctccatgt agccgcggcc cgcgatgggc
    15481 tttccttcca aggtgccgga cgcctcgatg gcaccctccc aataggtgac ctgtgtgctg
    15541 cggttcgtgg tcagttcctg ctcggtcatc cgaggaacaa gcgcaagcga aagacctttc
    15601 gacgggatgg acagccgcca ggtcgaggga tagcgtgcct tgctggcagg actggtccag
    15661 gtggtgaggg gctccagcat gaactccccg agtgttagat gctgagcccg gccatcgtgg
    15721 tcgataaaag tgccgcttga aacggaatca gatgatccgt cggaccgacg catgcgatag
    15781 agcatcagtt cagtgtgatc ggcaagctgg atgctgaacc aatcccatcc gacgagatct
    15841 ttgccgagat cggcagaacc aaactcatga tccatccaac tggttcccgt cacattgaac
    15901 gattcgttgc ccagcctgat tgtccccgag gtcgccagcc tcgttaacga gtagtagtga
    15961 gacgcttggc ccgactctgc gccttttcgg ctgacgccct ccttgccatg aatcacaggt
    16021 gccttttctg gcacgaccgt caagtcgatc tttagatcgt cgtctgaggc ctgaagggtc
    16081 tgtgacccca cctctgcttg ggaggattga gcgcgccatt ggtcgatcca cacgtgcaaa
    16141 cgttccgcct ccgccccagc cttgccgacg ccggcacggc tgagcttttc aaaaacatga
    16201 aaccgtccgt tcttcaaatc ggtcacggcg aagtgagcga ggtagagttg ggtaatggac
    16261 caccgtgagg gcaaagtttt gacttgttcc agcgggatgc cgcgacgaaa aaaggtcagt
    16321 tgatagccga atgggcgtcc gtcgttcgtc gcgaggttgc cggtgtagta ccaccattcg
    16381 gtgcggaact catcgtgtga accgtggtcg cgcgggaatg catatcgata gccttcccgc
    16441 gcgggcacga aggaggtggt gtccgccgcg atggaaggaa ggacgattgc gagccaaccg
    16501 aggaggaaga tcaatcgccg cgttgaagtc atatgtctgc tggtcacgat gcgggcgctt
    16561 ataacatgcg actgcatgaa gtcacaaatc gccagtagcg acaagcgatt tctgcgcttc
    16621 ttctggtcgt gtgtctcgcg gttgcgccgt tgacaatttt gtcagccatc ggctatcgtg
    16681 accgcatgca aaccgatcac gagcgtgatc agcacgaacc ggagccgtcc gaaaagagca
    16741 cgccattttc cattcccgag cacattccag tctttgcgct cccgaacgtg gtcttgttcc
    16801 cgaaaacgta tctgcccctc catatttttg agccgcggta ccgccagatg gtggatgatg
    16861 ccgtgatggg cgggcaatgt atcgggattg cgctgctgaa agaaggctgg gaatccgagt
    16921 attacgggaa tccgccggtg ttctcgaccg gatgtgtcgg tcggctggtc agcgtgcagc
    16981 cgctggcgga cggccggtcc aacattcttc tccagggact cgagcggttt gaaatcatcg
    17041 tggagtcgta tgagaagccc taccgtcaag cttcgattac gctgaagccc agagctgcgg
    17101 aggcctcgct cgatcctggc gtacgacagc gactggtcac ggcgctcgag gactatttgc
    17161 ggtcccgtca ggatgctccg acctggcagg gatggttccg ggaagatgtc agtgacgata
    17221 tactggtcaa caccttatcc acgtacctgg attgcacccc gctggaaaag caattcctgt
    17281 tggaagccgc cactctccat caacaggctc gccgcctcag cgatttgatc caattcctga
    17341 tgcacgatca tcatggggcg aagggctggg ggtgatgcac ggggcgattg cgatcgacgt
    17401 ctctcatctg tccaagtctt acgacggtca ccccgccgtt gcagacattt ccttccaggt
    17461 ctatgtcggc gaaatctacg gactgttggg tcccaacggc gcaggaaaaa gcacgacgct
    17521 gcgtacgctg attacgttgc tcgcgccttc tgccggacgt gcgacaattt ttggcctcga
    17581 ctgcgtacgc gatgcggatc gggtccgtcg agtgatcggc tacgtgccgc aagagcgagc
    17641 catcgaccgg tttctgaccg gcagagaaca tctcgagctg ctggccgcct tgtaccatct
    17701 gcccaaggac gaagcgaagg ggcggatcgc agagttgctc aagctggtgg aacttgaagc
    17761 gcatgccgac cgtccggcaa agacctattc cggagggatg aagcggaagc tcgacatcgc
    17821 ctgcggtctc ctgcccgatc caaaagtcct gttcttagac gaaccgacct tggggttgga
    17881 tgttcagagt cgactgcgga tttgggacta catccgccta ttgaaagccc gggggttaac
    17941 cgtggtcatg accacgaatt atctcgatga agccgaccag ctctgcgacc gattggcgat
    18001 tatcgatggc ggggtcatta aagtcatcgg ttcaccggtt gaattaaaga tcggattggg
    18061 cggcgacatc gtctccttga cgctcaagga ggccgagaga atctcgtcgc tcgaatcggc
    18121 cttgaggggc cagcctgcta tcaaggccgt cagggcaacg tcgaagggac tggacatccg
    18181 cgttgaatcg cccgagaagg cattgcctac cattctggag gtagccaacc ggttgggatg
    18241 tactgttgag ttcgtccaat ataaccggcc taggttggac gatgtgttca tggctcacac
    18301 cggacgacgc atcggagagg cagcggatcc tcatgttgtc gactaaccaa gctttggaac
    18361 tcggccatgc atgaatactg gcaagagatc agtgcattga ccatgcgttg ggtgctccgg
    18421 ctcagccgtg aaaaattcag catgctcttc accctcgtgc agcccatgtt gttctggctg
    18481 atcttcttcg gaaacctgtt ccagcgggcg gcggattctc aggtcacaca ggcgccgaac
    18541 tacattagtt ttttagcggc cggggtcgtg gtcatgaccg tgttgaacaa tggactcgcg
    18601 ggaggagtgg acttactgtt tgacaaagaa aacggatttc tagaacggct catgtctacg
    18661 ccaatccacc ggacgtcggt cattctgagc cgcttcattt ttgtcatgac gatcacgtcg
    18721 ttgcaagtgc tggtcattct cggagtcgcc ttcctcttcg gcgtccagcc ggcgaccggc
    18781 ttactgggta tcgccaccat cctgctgatc ggcatgatgt tcggcgtggg cctcacggcg
    18841 atttccatgg cgatggcgtt ttccgtaaag agccatggcg atttcttctc cgtgttaggg
    18901 ttcctttccc tgcccatgat attcttgagt tcagcgttgg ttccattgac ggcgatgccg
    18961 gcctggatga gcgtactggc gaagttcaat ccgatgacat gggccatcga cgcggtgcgg
    19021 ccgttgattc tttccggctg gagcgaggcg ctcccacatg tagggatggt cgtagtcgtg
    19081 atgattctct ttgatgcgct gtgcctctac ggtggtgcca aagcgtttcg ccgggccatg
    19141 ggatgagagg cgtagaccgt cggcatgttg accctcaaac ggaagggctg gtaagaaggg
    19201 tgggaatgat ggtcattcca gaaagtcaga tccgcgccct catccggctc ctggctgacg
    19261 aggacgagaa aatcgtccaa acgatcagtg gccggctcat cgatatcggt ccttcagccg
    19321 ttcccctcct gcaggaagca gaaatcgaac agcctgaaat ggcagagcgg atcgcgtccg
    19381 tattggaaga aatccgtggc ggaaagctcg aagacgaact gatcactcta gcggcgctcc
    19441 ccgacgaccg gatggacctg gaacatggct catttttgat cgcgcgctat gcttatcccg
    19501 gcttggacgt cgcggcctac agccggcagc tggacgaaat ggcgcaggag gtgcgtgacc
    19561 gcatcggacc acgcgcgtca ggcgaggaaa cggtgaagac cctcaatagg tatttgttta
    19621 cggaacaggg cttcaaaggc aacaccaaga actactatga ggtcgaaaat acctatctca
    19681 accgtgtcat cgaccggcgc actggaattc ccatcagttt gtccatcgtt tacctgctta
    19741 tcggcaagcg cttgcggttg ccggttcacg gtatcggcat gcctggtcac ttcctggtca
    19801 agtacgaatc cgaccgatac aaaatcttcg tcgactgctt caatggcgga gccctgttga
    19861 cggaaaaaaa ttgtcagcgt tttctgacgg aagccggcta cgggttcgat gagcgctatc
    19921 ttcagcacag ccctgtccgt gcgataatct cacgaatgat caaaaatctc ttggcgatct
    19981 atgtcaaggt gggtgatccc atcagacaga cccgtctcag caagttcatg gaaattttgg
    20041 gctgcgagat caaagaaggt ggattgtagc gaacccgcct caccctccga tcgaacttat
    20101 tcaaaacagc tggaatctta gaccttgatg cgcaggagat cacgcgcagc aacgatcggt
    20161 cccgcgaacg cctcagctgc ctgcgcctcc acgtcgtaac gctcggcaat ggggtagaag
    20221 tgcgagagta ccagctttcc tgacccggct tcctgggcaa cctggccgca ttggcctgcg
    20281 tgcagatggg ccggattggg ccggtttgcc ggaaacgaac aatccagcac cgcgagatcc
    20341 acgtcgccgc agagccgcac gagcgactcg caatattgcg aatcccctga gtatgcaagc
    20401 gcatggcccc ggtattcgat ccggtagcca agacaatgta aatgaggggt atgcaatacg
    20461 gtgcccggga taatcttcgt ggagcccaag cgcaggggcc gatcgctcag gtcacgatac
    20521 cggatcggaa acggcgcctg gtcaaatccc ggcaacatac cgaacatggt cttgaacaga
    20581 cgggtggtgc cccgtgggcc catcaccgtc aatggcggcc tgttgccacc ttcgaacttg
    20641 gtgtagatga tctcgtcgaa gaggtacgta atgaaatccg aaaagtgatc ggcatggtaa
    20701 tgcgaaaaca gaatgtgggt gatccggtgc cggtccacac cggttttgat caggttcatc
    20761 agcgttcgag ggccgaaatc cagcaagagc gtctgatcgg tctgaaccag atatccggct
    20821 gccgccctcg tgcgatggac atttgtgcct gaaccgagaa tggtgagatg catgctcaag
    20881 cccggcctcc tgtttggaaa ctgtcacgga tcctgagcag cagcgtgctg gcagtatgga
    20941 cctcttgcgg ggacagcgcc cgggcgaaaa acggtcggat aaaagcaata tgggcggcaa
    21001 agacttttcg aaacagggca ccgccttttt ccgtcagctt gatcttggtg cgccgcttgt
    21061 cgcctaggac ggactctcgt ttaatcaacc cttttgtttc cagtcggtct aacacgccgg
    21121 ttaaggtgcc ctttgtcacg agcgtctgag aagagagttg agaacaggtc aaccctgtcg
    21181 tgtcgccgag cgtgacgatg acgtcgaatt gcgagggtgt caggcggagc gatctaatgt
    21241 gccggctatc gatgcgagag aaggccaagt aggcttccac taatggacga agcacttgaa
    21301 gataggggtc gtctttgagg ttgggcaaat ccatgccgcc attcacaata gtccgaatta
    21361 gaactaccgg tcaaggacgg ccagtcgttt gacgcaaacg ggccgcgctc cataaaatgc
    21421 gttgcgtatg ctgagccgtc ccacgggcca ggctaatctc accgccatcg cggtccttct
    21481 cggcaccgtc atagcgggag tctgggtatg gaagcggctg tcgctcgaaa cacaagatta
    21541 tatcctcgat gaagcgctgc caatggccct acttgcagcg atgggcctgc ttgtcgtgtg
    21601 ggggatcgga cgggctgtcc acaggcgtaa ggatcgactg cgtcgccggg ccagcctgct
    21661 ggcgcgtttt tcccgggaaa cgactcagga acaaaagctg gaaacagcct ttgccctgat
    21721 cgaggccaac gaatatcgcg tggaaggatt ggaatcggtg gttccggccc tcaaagagtt
    21781 gttcgccacg acgttacaac gcgccgtcgg cgacaaacag catcgcctac gtggaatggc
    21841 ggccagtcat atcggcgttc tgcaggactt gagcgtcgtg cccttactgg tcaaagcctt
    21901 ggaggatgac cacgcctacg tccgttcctg tgcagcattg gcgcttggac gcttacgcgc
    21961 caccgagacc atagagcgcc tgaccacggt catgaaggac gattgggacc agacagttag
    22021 gagccgggct aaggaagcgt tggcacggat gggagcatga attcggggtt ctcgcgcccg
    22081 gtccgtcgag ggaagacatg gtgacctcgc cggaacagcg gaacatcgcc catgaccagc
    22141 atctctggga ccggatcctg cagcagtcgt aaaccagacg ccgtcatcta aacgtagaag
    22201 cgatattcgc gcgcctgtct gatcagagcc gaggaggatt agggagccgc ttctcgctct
    22261 atccaatgaa tgacggcgat ttccggacga cagttccagc ggaatgggag gaaggaccag
    22321 ccgaggccac gattcacata gagccgatgg cgagtacttt catgtcgtcc cgcgatgcga
    22381 ccacgacagc cgggggggag ccagaatgtc gggacccctg gaatctgcca ctgccctccg
    22441 tgactgtgcc cgcacaatat caggtcgacg gcatgcgcct cgtccatcat gtcgaggata
    22501 ttgggggcat gggcgaggag tagcgtgtag cggcggttgg ccggaggagg aatgcatcgc
    22561 agatccgcac gatgaagcga ggggtcgtcg actcccacga cagccagctc tccggatccg
    22621 ctcgggatga acgtactctg attgatcaat aatgtgattt tgtggcggtc ggccagctcg
    22681 gcatattgcg acagcggcac gccgctgtag tgatcatggt tgcccaacgt gacaaagact
    22741 ggtgcgatct ctcgtaattg agacagaaag cgaaacagcg gcggtaagcc ctccggcacg
    22801 ttcagcaggt ctccggtaat gaaaatccaa tccggccgaa gctcttggac ggtgaagacg
    22861 accacgtcat ggcgcggttg gagccgatcc acgtgcaggt cacttaggtg tacggcccgg
    22921 cgcccggcaa gaggcggaga gatgtgggtg tggtgcgtga tttcgggctg gtcgaggcca
    22981 atttcccagt tgggaaagag ccgaaagaag cgatagaacg gctcactcag actgtgtcca
    23041 atgcaggtac ggacccgatc gggccacgag gcgattcgtc tgatcatttc atgacccggt
    23101 ccagctcagc ccggtcatgc tgggtgacgc ggtaccctcg ttcatagagc gtgctcatac
    23161 ccttcatgac ttcgtcgaga tttctggtcc ggtaccactg gtcgaagcgg gcggtcaatg
    23221 tggccccggc tgcgccaggc ggtgcagccg catcaaaggc gagggacagg gaatggaaga
    23281 tgtcttgtag ctctgcggca ttgtactgct gatcgtcgtt atggtcggcg accagcgcga
    23341 actcgtatat cgtcaggctt acggacagca tggcctgctt tcttgcaaaa tcctctcgtg
    23401 ccggctccat gccgccggga taatgacggg gacagtcgcg ttcgggctgc agtgcgacac
    23461 tcaggcgccg attgattccc tgtgcggcgg ctggaggcgg ctttttctct cctttgtaga
    23521 accatgagag acaggtgctc gcctcggcga aggcttgttt gtcaatcgtg acctgcttga
    23581 cgatatggtg agtaaacgtc gggaagacac cgttgtcggc tacgaacaca ccccgccctt
    23641 cggacgagaa cgcggagaat gcaaggggaa gggcggcgac gatgttccac gtagccagca
    23701 tgactccgag tatatcatgg aagcgtagga ggtccgtctc acatggcact gccttatctg
    23761 accgcgaccg tcccgggaat cggcggacgg ctccgtacgg agcctgagga cttccaggtc
    23821 gacgaacgcc ctctgtatct cccctgtgga gaaggcgaac acctctatat caaggtgacc
    23881 aagcggctgc tctcgacccc cgatttggtc cgtcgaatct cctcggccgt cggcgtcaag
    23941 accaagggag ttggcacggc cggtctcaag gatgcgagag ccgtgaccac gcaaatgctg
    24001 tcactacacg cagtgacacc cgagcgtgtg gctcgattga aactcagtga ccagattctg
    24061 gccatcgaag tgctcggacg gcacggaaac cgactgcgcc cgggccatca cgctgggaac
    24121 cggtttcggc tggttattcg ggacgtagcg ccccacgcga aggaaaccgt acctacggtc
    24181 ctcgctgtac tgttgcgtcg cggcgtgccc aacttcttcg gacctcagcg gcaagggaag
    24241 aacggtgaaa actaccatat cggagctgcc ttactcacgg attcatcgac gcgggctcag
    24301 atgagccgct ccaagcgcat gtggtatctc aattcctttc aatcccatct cttcaatcag
    24361 attctcggac gccggctgga acaccttgat tgcgtgtggg tcggtgattg ggccatgaaa
    24421 atggagaacg gcgcctgctt tctcgtggaa gacgctcagc aagaaaaggc gcgggccgac
    24481 cggtttgaga tcagtcccac cggtgtgctg ttcggatctc gtgtgtcttg ggcgaccggt
    24541 caggcgggtg agatcgaaca ggccgtcatc actgaacaag gctcgacccc tgaggccctc
    24601 attcaatcgg ccaaagcctg tggatttcgc ggggaacgac ggtctttgcg tgtgccattg
    24661 ggggagctgg agtggtcgct cgatgggtcc attctgacgc tgtcgttcgt tcttcccccg
    24721 ggtgcctatg ccacgagcgt ccttcgcgaa ctcatgaaaa taccagaact gggttgagtg
    24781 gccgggattt tttggttccc ggtcgatgga ccgggcagtc agccactgca tggtagaatc
    24841 taagtgaggg ggggtcctgg cgatgagtgc tgccacccaa gaaaccgtcg tgctgagcgg
    24901 gcatatcatc gattcgctca tccttgccaa ggtgctggat accatcctga tgatgggcgg
    24961 gaccttcgac ctcgagcagg tgtcgatcgg caaaacccgc aacgaaccgt cccgtgctcg
    25021 catcgttgta cgagccggtt cggtaaagct gcttgcggac atcctgaggg ccatccaacc
    25081 tcatggggca tccgtggaag gggaggtgga ctgccagctc aagacggccc ctggtgacgg
    25141 tgtgtttccg gaggatttct atgcgacgac ccacttgccc acgcaggttc gactctcggg
    25201 tcggtggatc gatgtggacg ccatcgaaat ggatttgggc atcgtggtga acaaggacag
    25261 gaccaaagct gtcgccgtcc cgatggccgg cgtgaacgtc ggtgacgaag tggtgactgg
    25321 ccgaacggga atccgcgtgg tcccgttgca acggcctcaa gaacgcgacg tattcagttt
    25381 catggagtcg caggtctccg ccgagcggcc tcatggacat attatcgccg acgtggcgaa
    25441 acggatggag gtgctgcgtg acggtcaccg gggcggaaag tccgggtgca aggtgttggt
    25501 tgccggagga ccggcgatca tccatgccgg agggcgcgag gcgctgacat ggctcatcga
    25561 ccagggattt gtccatgtcc tgttctgtgg aaatgccctt ccggcacacg acatggaggc
    25621 cgatttgtat ggcacctcgt taggctatgg actttcggcc ggcagagtgg ttccgcatgg
    25681 tcatgagcac catttgcgca ccatcaaccg cattcgcggc atcggcagca tcgcgaaagc
    25741 cgtcgagacc ggcgtcatta cacacggcat catggcctcc tgcgtgaggg agcgagtgca
    25801 gacggtcatg gccggtacca ttcgagacga tgggccgctt cctggcgtca tcaccgattc
    25861 cgttgcggcg caggccgcga tgcgcgcagc gattcccggc gtgggcctcg ccttgctgat
    25921 cgcctctacc ctgcatgcgg tcgctaccgg caatctcttg cccgcgacca ttccgacggt
    25981 gtgtgtcgat gtgaatccgg cggtgccgac gaagctggcc gatcgtggga gtttccaagc
    26041 ggtagggctt gtgatggatg ccgcctcatt tttgcgcgat gtggcgagag aactcgggtg
    26101 gaaaggatga gccggttact cgtctgtccc ccggactatt tcagtattga ctacgagatc
    26161 aatccgtgga tgcgacgctc aaatgtcgtc gccccggagc gagccattca acagtggcac
    26221 cggctgatgg aggtcctcga gcgggacatc ggcacggtcc tggaacggat gaagccggtg
    26281 gccgggcttc cggatctggt ctttaccgcc aacgcgggcg tcgtcgtcgg ccgtaaggcc
    26341 gttcccagcc gtttccgcta tccggagcgg caacgggaag aggcgcatta cgaaggctgg
    26401 ttccgtgcac acgggtatga aatcattact ctggacccgg atatgttttt cgaaggggcc
    26461 ggcgacctgc tgggattcca cgacggctgg ttcggcggct acaggcagcg ttcggacatt
    26521 cgcgcatttc ctgttcttag cgaattgttc gggaaagaga tccttcctct ggaactgatt
    26581 gacggccggt tctaccatct ggacacctgc ttttgtcctt tgagcggggg agatctcctc
    26641 tattatccgg cggcgtttga ccgctatgcg caggcagcca ttgccgatcg catccccact
    26701 gtgtcgaggc tgcctgttcc tgagcaggaa gcgctcaagt ttgcctgcaa tgctgtctgc
    26761 gtgggaaagc acgtcgttct gccggacggt tgtccggaga cgatgacctg gctcgagagc
    26821 cgcgggtatc tgccacaccc ggttcctctg gatgaattta tgaaatccgg agggtccgca
    26881 aaatgcctca cattagctct ggactgagcg tcaaccggtt cggacacgca ccctctacct
    26941 tccgctgcga aacagaaagg caccatagag gccggcgatg gcaatcgtca ccaactcgat
    27001 cgtgatcacg acccggctga cgatggcatc gggcgtcggc ggcgacagga tgaaatgcaa
    27061 ccccaagaat tggggaggct gtgccggagg tgtctcccaa ggcgggaaaa ggatggctaa
    27121 gaccagaaga gccaccatgc catacaagac atgaagattg aggctttccg gagcgacgga
    27181 tgggcgggcg atcgctcggg aggccccagc catctggctg ccgcaatgcg tgcagtagcg
    27241 atagatttca ggccactcac gccggcatga cgggcagacg ttcattgggg tccgtgcaat
    27301 gtacacggac cccgtccctt ttccaactat acaaccaggg gcttcgatca aaaatgatag
    27361 ctgacgccga aggcgacatg gaccatgttg taagtggcgt tgaaaccgaa cagattgaaa
    27421 ctgctgcctg gcggagtttc ggggaaattg aaccggacgt ggttgtattt tccttcggcg
    27481 aacatcgtga catgcctggt gacataataa cggagtccga tctgcgcgtt gagtccaagt
    27541 ttcgtgctgg actgtgagtt atctgaacta agcgcgggat ccttgatctt cgcgacgaag
    27601 attccgggtc caattgcgac gtacggttgg agtctcgtct tatggtaacg gaagctgaga
    27661 ttcagcggtg caagtgtaag gacgctcata tggacgccgg ccagctcggc ggacccgaca
    27721 ggaccattcg gaccgcggaa ttcgacggtt tgttgtttga tatgaggcgt cgttcgatag
    27781 atttctgctt ctaggccgaa ccagggggcg gctctgaaat agtgtccaat ctttcctcca
    27841 tacataatgg aggatttgag agcctgatcg gaagtggtcg atccgggaat aaaactgccc
    27901 gtcagatcca catctgaaag gcccttcccg atcgatggga gggtcacgcc gagctggccg
    27961 gccacatacg tttctgcctg cgccgtcgat aacgacagaa tggccggtgc gctgatggcg
    28021 agcagggttg ctccgatgag cgcagtaatc gttcgcatgg tgtacattgt cctgccctca
    28081 ctcatggtag gaatcgcctg tcccatccta tccgtcgtgc ggtctggcgc gaagaagttt
    28141 gtgaaatccc gctgtggtcg agcaatcgat gtgccataag aaaaaggtgg aaaggccgta
    28201 gaaaacgcct gtgcatgttg gtcatggctt tgtgctgtcc tcggaaacag tcggcgccgt
    28261 acacttcgtg gcaggccggt ttgacaggag aaacatggcc tttctataat ccgcgcgcct
    28321 gatcacacag cacaaacccg acatcagccc atccaccttg agcccgtcat tcagcactat
    28381 gcagccgatc cagcatatct cggtcatcgg tgccggagcg tggggaaccg ctctggcgaa
    28441 acatcttgcg gagaagggtc tgcagacgat cctgtgggca tacgaacggg acgtgctcga
    28501 atcgattgcg tccaagcgtg aaaaccagct cttcttgccg ggagtggcgc ttcccccctc
    28561 actccgggtg accaacactc tggcggaagc cttggagcaa tgtgacggcc tgctctttgt
    28621 ggtcccgtcc catgtcgctc ggcaggtgct gcaacaactc gcccccttgc tctctcgccc
    28681 cctccccatc atcagtgcga ccaaaggcgt cgaagaagat acattcaaac tgatgacgca
    28741 agtggtgacc gaggtgctgc cgcctgatct ccacgatggg ttgatggtcc tgtcggggcc
    28801 cagctttgcc atggaagtca gccagggcca gcccacggcc ctgtgtctag ccgggaccaa
    28861 tgcggcgctc gtcggatcgt ttcaagccgc tcttatcacg tccacgcttc gtatctatgc
    28921 agacagcgac gtcgtcggtg tgcaacttgg aggggcctta aaaaatgtca tggcgttagc
    28981 ggccggggtt gtcgacgggt tagggttagg gcacaacacg agggccgcca tgatcaccag
    29041 aggcctggcg gaaatggtcc ggctcggcat ggcgatggga gcggatccaa gaaccttcta
    29101 tggcttatca ggcgtcggcg atctggtatt gacgtgcacc ggtaccttga gtcgcaatca
    29161 tcatgtgggc gttcgactcg gccaaggcga tacattggac gccgttctcg cggggatgca
    29221 ggccgtggcg gaaggggtgc ggacgagccg ggcggctgcc gggttggcgc tgcgacacgg
    29281 ggtcgaaatg ccgattgtcc gggaagtcaa tgccgtgctc tttgaaggga aatcgtgtcg
    29341 caaagccgtc agcgatctca tggagcgggt cgccaaacct gaaaagggcc acccatgaat
    29401 cgtgaacggc ttgagcgcct gctcggcgag gtcaagagcg gcaaggcttc cattgccagc
    29461 gctcttaagc agcttcgatc cctccccttc gaagatctcg gttttgcttc gttggatcat
    29521 catcgttcga tccgtcaggg atttcctgaa gtgatcttat gtgaaggcaa gacgccggcg
    29581 cagatccgcg gcattgcggg atctctgttg aaacaccatc gaccgtttct cgccacgagg
    29641 gcgactcgcg acatcgcggc cgtcattaaa cggatcgatc ggcgggcggt ctaccacgaa
    29701 tccgcccgga tcgtcgccgt gcgcgatgcg cagcgacggc ttcatggccg tgtcctcgtc
    29761 attacggctg gaacatccga tgtccccgtc gccgaggagg cccgtatcac ggcggaagtc
    29821 atgggcagcc gggttgagac gctgtacgac gtcggcgttg cgggtattca tcgcttgctc
    29881 ggccggcatc aacagctcac cgacgctcat gtcatcgtgg tggtggccgg tatggacggg
    29941 gtcttgccga gcgtggtcgg aggcctggtg gagtgtcctg tggtggcagt gccgacgagt
    30001 cgcgggtatg gggcgagctt caacggactg gccgcactgt tgaccatgct gaattcctgt
    30061 gcggccggcg tcggggtcat gaatatcgac aatggatttg gggcgggctg tctggcgcac
    30121 cgcatcaacg ccttcgggat gaagcaggat cggtcaggct gatgttccag gcgccgcgac
    30181 gagttacttc acctccagtg tcccgcgttt tggccaggac agcatgagcg tccgcggccc
    30241 cttttcgcca ttggaaagga gcttcgcccg cagttcgaat tgatagattc ctgcctccgc
    30301 cggcgctttc gtgtggtcca tgccatccca ggtcaggacg acgggaatcg tctttcgatc
    30361 ggtgttgata ggcgtgatcg ccggcgattc gagcggcttc cgcagggaaa gaaagcggat
    30421 ggatcgtttg gagggagagg tgaccagtga tgacacttcc aagatggtcg ccccctccag
    30481 ttctcggggt aattgcacga gcgttgaaaa ttccagagac ccggatgaca cggtgtaagg
    30541 attgggactg atcttcaatt caagcacttt caattccggt tcaggccgtg gagcccttgc
    30601 cggctttgat ttcgccgaaa gggcgaccgg atgcagcagg aagatacaag aggccagggt
    30661 gcatgtaaga agcgcacgag tgagatggaa gcaggatggt ggcgtttcag tggcgggtat
    30721 ggttcccagc atggagatct ataggctcag gtttccagga ggcaggagag accgattgcc
    30781 gcagtgggat aacacagtgc acagaagctg tcaatgcgca ctcatcattg ccgtctcaag
    30841 ggaccgttgg gtaagatgag ccgactttta ccaccagcac atcaaccatg tctcggcggt
    30901 ggaagctggg aacgttctcc tcatcgtgac gcgaaggcgc atcccagcgc accggtatgg
    30961 tcacgtacga tgagcgaaag gatggcgtga gcgcacacct ccactttgac tgcttttccg
    31021 gaatcagcgg ggatatgacg ttgggtgcgc ttgtggatgc gggtgcaccc ttcggtgagt
    31081 tggtcaagag actcaaacga ctcggcgtcc cgggctttac actccgtaaa aggcgagtgc
    31141 agcgtggcgc catttctgcg acgaaggtag acgtcgtcat cacgtccggt cttctgcgac
    31201 cctggccgct cagccgtatc cggaagacac tttccgagag ccggctctct gagaaaatca
    31261 agcagcaaag ccgcgcggta ttcgagcggc tagccgaggc agagtccaaa gcccatcgcg
    31321 tcgcaatgga ccatgtgcat ttccatgaag tcggactggt ggattcattc gtcgatatcg
    31381 tgggagggct catctgttgc gacctgctgg atgttggtcg tgtcacggcc tctgcggtca
    31441 atgtcggaac cggcacattg caatcagcac acggcatcat tcccgctcca ggccctgctg
    31501 tggcgatact cgcgaaaggc gtgcccatat acagtgccgg acctgcgcgg gaattgacca
    31561 caccgaccgg cctcgccttg ttacgaactc tcacggagga cttcgggccg atgcctcaga
    31621 tgaaaccggt cgccgtgggg tatggcgcag gggatggcaa ccccgaaggc tggccgaatg
    31681 tgcttcgggt gtttttgtcg cgagacgccg gctctgatgg acggggccgc gacaccgtgg
    31741 cgcagataga aacgaacctg gacgatgtca accctcaaac ctatgaacac atcacggagc
    31801 gcctcttcga tgctggcgct ctggatgtgg cgctgattcc cgtgatcatg aaacgcgggc
    31861 gccccgcgat cgtgctgact tgccttgccc ccttcacggc ggtcgattcg ctcttgaatg
    31921 tcctcttcga agagaccacg gctttgggag tgcgtgtgca agaagtcacc cggcacatcc
    31981 tttcacgacg gtttacgtcg gtgaagatcc gcggcggcac cgtccgcatc aagatcgcgg
    32041 cggtcgacca tacgagcagc aaggccgctc ccgagttcat cgattgtaaa cggatagccg
    32101 aacgcaccgg ccgtcccgtg aagacggtat tggaagaggc gacattggcc tatgcgaaga
    32161 cccggagcac tgcaaaggac cgtccgtgac gctgctggga tacgtcctgc tgttcggaac
    32221 ctctgtcatc attaccctgg gcgcgtgtgc gctgtttacc aacgcggttg aatggctggg
    32281 gaagcggttg aatctgtccg agggcgcagt cggcagcgtg ctggccgcga tcggaacgac
    32341 tctgcccgaa acctctatcc cgatcgtggc gatttttttc ggcgccaccc ggcaagaagc
    32401 agaagtcggc ctgggcgcca tcctgggcgc gccgttcatg ctcagtacct tggttgtccc
    32461 tctgctcgca ggacttctct tggtctatgc gcagttgggg aaacgcggcc gttccttccg
    32521 tctcaactac aaagacgtca cggtcgatct gtcctgtttc gccctggcct acacagtcgc
    32581 gatggcctgc accttcgtgc cgtccaggcc gttgcggatc ctcgtggcag tggggttgct
    32641 gggtttgtac ggctactacg tgaaaatcaa attcagcgac gatgatgccg gtggtgagcc
    32701 gagcgatttg agtccgctgg tgttcgccaa aaacagcgcc cggccgtcct atgccctcat
    32761 cggactgcag gcggtggcgg gcctgggagg tctcgcgctc ggcgcgcatt tgttcgtcat
    32821 ggcggctgat tcaacggcgc agctgctcgc catttctccg ctgatcttgg cgctgctcat
    32881 tgcgccgctc gcgaccgaac tgccagagat gtccaacagt tttctctggc tctatcagaa
    32941 aaaggatcgc ctcgccgtgg gcaacgtcac aggcgcaatg gtattccaag gcaccgttcc
    33001 ggtctcggtc gggttggtcg gaaccgaatg gacgctcgcg ccgtcggcgc tcttgacgat
    33061 ggtcctggcc gtcctggcgg cagggagtct cttgcttcaa gctgtctggt ccggccaatg
    33121 gcggccgtgg ttgctcacca tcggggcgat actttatata ggatatgcat gctatctgta
    33181 tggctattga ttcagggact ctgaaaaaga gtacccctgc gcacagactg gcctcacgtc
    33241 agccccagcg ccttgccgtc ttcacccgga agcaaccgtg aaaaaacctc aatcgccatc
    33301 tcgcctcccc atgctcggga taacgatggg tgatcctgcc gggataggac cggaggtcat
    33361 tgcgaaggcc ctcgcgggaa ccgcgctgcg ccggatctgt gttccccttg tcatcggatc
    33421 gcaggaggtc atgcagcgta ccgtgaagca gttgaagctc aagctcgcag tgcgtcccgt
    33481 ggagggccac aaccccatcc gtctgagcgg gggacacgtc gctgtactcg atcctctcga
    33541 gcggccgctt ggtccctaca agctaggcgt ggcggcggct gaaaccgggg cggcatccgt
    33601 cgcattcatc aagacagccg tgcgtctggc ccaatcggaa tgcattgacg ggatggtcac
    33661 tgggcccatc aacaaagaag cgatgaacat ggccggctgc cattttcccg gtcataccga
    33721 gctgctggcg gagctgacgc agacgccgga atccggcatg atgatcatcg gcggccggct
    33781 caaaatcatg tttgtgacga cgcacatcgc gataaaggag cttcccgccg ttttgacgca
    33841 agccaagatc gagaagggca ttcggcttgc gcatctcgct ttgacgaccc tctttggcat
    33901 aaaaaagccc cgcatcgggg tcgccgcgct gaacccgcac gccggtgagc atggactgtt
    33961 cggcgacgaa gaaatccgcc tgatcggacc cgccgcacgc gccgctcaag ccggggggat
    34021 tctggccagt gatcctctgc cggccgatac cctctttggc aagaccgcca agggcgatta
    34081 tgacgccgtc gtggcgctgt accatgatca agggttgatc ccgcttaaaa tggtggcgtt
    34141 tggaagctgc gtcaatctga cagtcgggtt gccgatcatc cgcacgtcgg ttgatcatgg
    34201 caccgcattc gacattgtcg gaaagggcat tgctgatccg ggaagtctga tcgaagcggt
    34261 taaattggct gcgtcgcttg cgcgacgtcg aatgtctcac gctcgagcat aggagaacac
    34321 gcccaccatg ccattggatg tcaacaatgc gctgctcgtc atacagcagc aactcaagga
    34381 actcgatgtc atttatcggg acaccatgca gatgtccctc aacacggtgg ccgggacaga
    34441 gcgagtcgca aaatggcgtg ttcgaacggt gtcgttgctg gccgagtcgg ttggggaaaa
    34501 ggcggcgcag gactttgccc ggcttcagcc cggaatggtc tttacgaatg accttgtcga
    34561 ggaattcacc gatctcgtcg actgctttcg cgtgcctctc aatgcgctgg ccaagcagct
    34621 ctctcagacg gcccagcgtc cggctgatgg gggctcctag gtgcccggcc cggctttccc
    34681 gcgggccaac aagcgactgg gacagcactt tctcatcgac cagaatatcg tccgaaagat
    34741 cgtggccgcg gcggaactga ccccggatga gacggtgctc gaagtcggac ccggccgggg
    34801 tattctcacg gaggcccttg cttcagttgc ccgtcgggtt gtggccgttg agatcgatcc
    34861 gcagttgcac cggttgctgc aggcccggca gagcgagtgg aagaacgtcg aagtcgtgtg
    34921 cgccgatgcg ctctcgtatt cgctcgaggc agtggccgcc ggtactgtcg tcgtggggaa
    34981 cctcccctac tacatctcca cacctctgct gttcaaattc ctcgagcatc gccatcgctt
    35041 tccacggctg gtgctgatgc ttcaaaatga agttgccgac cgcctggttg cgaaggccgg
    35101 cacatcggca tacggcgtct tatcggtgat ggcgcaatat gcggccgaca tttcgaaggc
    35161 gttcacagta tcggccaatt gttttcgtcc tcgaccggag gtgggatccg cagtggtcgt
    35221 gttgcgcacg aagcaggaag ccctcctatc accagaagaa gaaccggctt ttgccgctgt
    35281 ggtgaaggcg gcgtttgcgc atagacgaaa gacattggtg aactcactcc gcgatgaggg
    35341 atacgaccca caggcggtca tagatgccat cagcgcagtc ggcatcgtct cgaccgttcg
    35401 ggcagagaca ttgagtctcg acactttcag tcgacttgcc acacgcctcg atcgtcccaa
    35461 gccgtcataa acacatgtca tgatgacagt cgtcgtttga attggttgac cccctgtgtt
    35521 agcatccggc acatgggcgt aaccagctcg gccaagcggg gcgagacccg ccgcgcccct
    35581 gccccccctt cccatctcaa gcgtgaagtg atcggcgtca tgctgattgc actcagcctg
    35641 ctcaccctat taagcctcct gtcctttgtg ccgggtgagc cgaaggtcgt cgccactcca
    35701 gcggcgacga tgaccccacc gactcacaac ctgattggct ccgtgggtgc ggtcttttcc
    35761 tctctgttgt tctctctcat cggcggtgcc gcctatctgt tccccatcct gttgggactg
    35821 ctcgggggcc ggtgttttac ccagagcgat ctttcaatca gactacgtaa cgccggagcc
    35881 tcactggctg ctctgttgtt cctgagcggt tggcttcact tggaagtgac ggcggtgccg
    35941 accgtctcaa gcggaatggt ctttcgaggc caggcgggag gcctcttcgg acaactgctg
    36001 gccgagggat tgcgtgccta ttttgcgaat accggcgctc atattctcat catctccggc
    36061 tttctggtcg cgctgctctt caccgcgccc ttgtcgctgg ctcagctcgc cgtgcaagcg
    36121 ccacagtggg gacgaaacgc gtttgcacga gtgaatgcgc tgatgccgaa gcggaagcag
    36181 gacgcggtcg tgccggatgc agcgaagccg accaagacgc gaaagccaaa gtcacttcgt
    36241 gccgtcattg aagaagtcct ccccacaccc gaagacaacc cttccgccga ggtgtttccc
    36301 gtgacgccgc caacggtcgt tttgcctgag ccgcgtgaag tggttgaacc ggagccggat
    36361 gcgcccctcg tcggcaaagt cgaggacacc ggcgactatc aattgccgga tgcggatgaa
    36421 ttattgacgg aaccctcagg ggtactctcc cgcatcaccg acgaggagtt gaaggggcag
    36481 tcggacattt tgtcgaaagc gttgctgagt ttcggcattg aaggcagagt caccgaggtg
    36541 cgcccaggtc ccgtcgtcac gatgtatgaa ttcgagccgg cgcccggtac caaggtggcc
    36601 aggattgtga atctcgcgga cgatctggca ttggccatga aagcgatcag tttgcgcatt
    36661 gtcgccccga ttccgggaaa atccgtcgtc ggcatcgaag tgcccaactc gcagcgcgaa
    36721 accgtttcca tgaaggaagt cgtcgccagc gacgccttta cgcgagcgcg ctcgaagctc
    36781 accctcgccc ttggcaagga catcttcggg accccggtca cggcggacct caagacgatg
    36841 ccgcatttgc tcgtcgctgg cgcgacgggt gccggcaaga gcgtcagctt aaacacgatg
    36901 ctaatcagca tcttgctgtc ggcaaaaccg aacgaagtga agctgcttct catcgacccg
    36961 aagatgctgg agttccaaac atacgacggc attcctcatc tcctgagacc cgtcatcacc
    37021 gatcccaaat cggcggctcg cggactgagt tgggtcgtgc aggaaatgga gcgccggtac
    37081 aagctgctgg ccgaagccgg tgtgcgcaac atcgattcct ataaccggaa agtcgccggc
    37141 gctcagggcg tgctgggtga cgccggtacg gaagcaaagc ccgaccagcc cgagctcccg
    37201 atccggtttc tctctgaaga ggagcgcctc gccgccggcg agacggccgt acaggacgga
    37261 agtcccggat ccttcatgcc tcctccgacg cctccggaac cgttacccta catcgtggtc
    37321 atgattgatg agcttgcgga tttgatgatg gtcgcaccga aagacgtcga ggacaaaatt
    37381 gcccgcctcg cgcagatggc gcgggcttcg ggtattcact tggtgctggc cacacagcga
    37441 ccgtcggtgg atgtgctgac cggcttgatc aaggccaact ttcctgcccg cattgcgttt
    37501 caagtctcgt ccaaaacgga ctcgcggacg atcctcgacg ccaacggagc cgaggcgtta
    37561 cttggtcgag gcgatatgct ctacctcgca tccggaaccg ggcgtctgat gcgtctgcac
    37621 ggctcgttcg tctctgatga tgatgtgcgc cgcgttgtcg aatccgtcaa ggcacaggca
    37681 ttgcccagtt acaatcagga attgcaatcc ttgaagcagg aagaggcgaa ggaggaagaa
    37741 gcgcaggacg aagtctatga gcaggccaaa gagttggtac tctcgacggg gcaagcttca
    37801 gcctcactga tccagcgccg cttacgggtc ggttatccgc gcgccgcaag aatgatcgag
    37861 cagatggaag cggacggtct cgtaggagca gcgggacgcg acggccgtcg tgaagtcatc
    37921 ggacggcgcg gccctgttgg agacgcccac gaatgatccg gtggggatgc atggggtgcg
    37981 ccgtcctgtt gacgatcggc gtctcggtga cggcgtgggc tcaagacgtc acggatgacc
    38041 agtctcgcaa agaagtgcgg gaagtggtga agcgcctgca atcgcgctat gagaagacca
    38101 gagatcttca ggcggatttc gtgcaaaaga cgactattga aggattcgaa cgaccgatga
    38161 cctcatcggg caaagtctat attaaaaagc ccggccgctt gcgatggaac tatctggagc
    38221 cttcgaccga ggacatctat gtggataggg acgatgtgaa agtgtacgtc ccggagcaca
    38281 aacaagttct ggtgggaaag ctgacgcaaa tggctgcgtc aaaggcgccg cttgagctct
    38341 tacaaggcgc cgcaaaactg gacgcctcat tcgagcccga gccgacaccg ggtaacggcc
    38401 ggggaattgg gggagtgagg ctggtgacct tggtgccgaa ggcgcatgac ggtgacgcgc
    38461 atcggaccgt gcagaaaatc gtcgtcgaag tgtttcccaa gacctatttt attcgaagct
    38521 tatggctcta cgagtcaagc ggcaatgtat cgaatttcga attttcctct ttgcagtcca
    38581 acatcgggct cggtgacgat ctttttgtgc cgaaactgcc gcccgatgtc gaaatcgtca
    38641 aggctccggt actcggccag ccctgattcg tgcaggccga cagccgtgag ccgggaaggg
    38701 aacgtctccg atgagtacag cactgctgat gacggtcgcc gaagccacgg cacaagcggt
    38761 cgggaagctg gacgtggatc gattacacca cgaatattgg gaccagaatg aatttgtcgt
    38821 tattccgcaa tttcttccga gggacttcgt cgaggcgacg ttggttccgc aggcccaggg
    38881 cgtcaaggga catctgaacc ggaattatat cccgggccat aaaaagggcg gcagcgtgag
    38941 ctattacacg gtgcgggagc aggcgccgct ctttctcgac ttgtaccgct cacccgactt
    39001 tctcgcattt cttagccggc ttaccgaagc caggctcatg ctctgtcctg acaacgatcc
    39061 acattcgtgc gcgttgtact attacacgga agccggcgat cacatcggat ttcattatga
    39121 caccagctat tacaagggag cccgttacac gatcctcatg gggctcgtcg acaagtccac
    39181 tcagtgcaaa ctggtgtgcg aactcttcaa ggatgttccc gaccggacgc cccgcacgct
    39241 cgaactggtc acgcaaccag gcgatatggt gattttcaac ggtgacaagc tctggcacgc
    39301 tgtcaccccg ctgggagctg gagaggaacg gattgccttg acgatggagt acgtgaccaa
    39361 tccggagatg ggccgcttca agcggctcta ttccaatctc aaggactcgt tcgcctattt
    39421 cggcatccgc ggggtcttca agggaaccct cgcgagttct aggaaatctc gctaatcctg
    39481 cttttgcccg ccttccgtcc aatcgagtca cacgccgctc cgtcagggta ccggtcgtaa
    39541 cgagacagct tcatggtgcg aggtgtctac ggagtccccg cctatcttcg gctggacctt
    39601 catggattgg cccaacaatc tgggccggcc ggcccggagc ggcttcgtac gtttggcaaa
    39661 aggtccttgg tgcatggcct ccagttccgt caatgaggcg cgcactgccc ttgccacgag
    39721 ccaagcgagt gtcagtcccg gcgtccagta caccgcgtcg cgcatttgtt cgacgaggtg
    39781 cgaaggcaga ttaacggtca accgaaccat gcgtggcgct tccggcttgt gcttcaccgg
    39841 tccggtgctg gtaacggatg ttgcacgatg aaacaggatg tctgaagcac ttggggtcga
    39901 gaccgtggtg gttggcatcg tcttcctcct tgtcatgatg tatgtggggc gcacaacacg
    39961 gcgtcgttga agatgggcac gggaaggtcg cgtgccgtcg gagattgaca cgcgaccttg
    40021 ttggccgaga ggcggggcga cggtgtgcct ccggatgggc acaatccgga ggaacgtact
    40081 tgctgacagt cggcgtctac cgcctgtcac ggtactatct gcgccgcatc tcgccgttgt
    40141 tgatcgaagg accgctcgtg gcgtcatgcg gcatcgctca gcaacagcca tgccacatcc
    40201 ggcagttaga attccatgaa gaatcctagg taatccagct gagccgaggc gcaggcgcaa
    40261 ggaaaggtat ccgaaacact acaggcgtta tctgaggaga tacatcacga gaaggccggc
    40321 ttggaggttc gaagatgtcg gaatgctatt ggtgcaggtg cttcatcgcc gccgcacaga
    40381 ggataatgaa gaagcccatc cataaggccg cacgctgaaa aggaatcact tcgtaggttt
    40441 cgccctcttc agcgtcatca tccgatttga agatgacggt gtagaggaac agcgtcagga
    40501 ggcagagcac gaggaagagc ttgatgaaga ataccgtcag gtattttgcg tgatgttgga
    40561 cacctgaacc ggtctgtgag gtgaggactt gattgacgta atggaggttg atccctcccg
    40621 taaacaagag gatcacaagc aggattcccg ccactttttt ataatgctga tagaagacat
    40681 ccgtgatggg cttgatctgc tccctggcga cggccttctg gaggccaggg gtgacggcca
    40741 tcacgaggaa cgccagtccg ccgatccaaa tgaccgcgga caacagatga aaccagtgat
    40801 tcacaatcgg aatgagcgta ttgagatcgg tgacgatgct ttggaaggcg tccatagaaa
    40861 tatttattcg tagagtccgg agcgtgaagc gagcgtgcga ttgttcatgc agtcattcgt
    40921 acgagagacg cttcaccaaa gacgagacat aggtttagaa cttaaagacc ggtgcatcgt
    40981 tgccgaagcg cttttttcga agctcttcca tgagttcgat ggtcacggtt tcgatgccgt
    41041 gttcccgcgc atgcttctcg acgcccttct tcaccatggc gcggagaaaa tatgggatcc
    41101 ggctcaatcg taatgaggac gcatcgtccc actgcacggg tgacgcctct tcgacgttga
    41161 acgcgacctt gatcttctcg ccgcctttcg gcgtgtaaag gcaccactgg tcctcatcca
    41221 gccaatcccc atgatcgaca taaggccgag cccggcagcc tccgcagatg tcggaatact
    41281 cacagtcgcc gcacttgccc ttgagctggg gataacggaa cgaattgaag atgtcggact
    41341 tctcccacag gtcgacgaag ctgttctcgc ggacattgcc tgcagagagc ggcatgtagg
    41401 gacagggagt cacttcaccg ttaggcgtca cacgggcgta attagtcccg gccaggcagc
    41461 caccgcccat gtatcccgtc gccttcgtga tcggtgaatt cggatcattc tcataggcca
    41521 gccgtttgaa gtgcggggcg cagcgggccc gaaccaacat tcctttatac tcatcctgac
    41581 acttcaccag gtagcccagg acctcctcgt attgggccgg agtaatgtcc gtcaattctt
    41641 cacctctgcc ggtgcagacc atgaagaaga cgttgagtac ccgtgctccg aggccatggg
    41701 cccattccac cacagccggc agttcctggt aattcattgg ttgcgcgctg aagtgcactt
    41761 gaaactgcag cccgttgcgc ttgctggctt caatgcccgc caccgccgct tcccacgctc
    41821 caggtacacc acgaaaagca ttgtgcttct tcggatcaag tgaatcgatg ctgatgccga
    41881 cacccatcac cccgatcgta accaactcct ttgccagctg atcgtcgatc agcatgccgt
    41941 tcgtaccgaa caccaccata aagccaagtt tcacggcata acgcgcgatg tcgagaatgt
    42001 cgggacgcac caacggctca ccgccggtga tcaccaacag acatcctttg ttgacctcgg
    42061 cgatctgatc aatcaggcgg aaacactctt cagtcgagag ttcatcgtca ccgccggccg
    42121 ccttcgtggt cgcatcgagg tagcaatggt cgcatttgag gttacagcgc ttcgtcagat
    42181 tgagcgcgac caagtagggt ttaaaatcgt cgacggtccg gccgtcatat gggccttcgg
    42241 ccagcggctt tggggtcagg aattgcgtga gctggttctg aagggacccc aacgctcctg
    42301 agagggatgg accgttgaag ataggaaggg atttacccat gagcgtggtg aatctggagt
    42361 agagaaatcg ctagaccagt gaattcaagg gaacaagcag ccggccattc gacatgacgc
    42421 atggcgctaa gctctacctt tgcccgtgag attggcgatc atgtccttga ggttgccggt
    42481 cttcatggct tcttccatgt agcccttcgc ttgctcgatc ccttcgttgg cgacttccaa
    42541 cgagatgtgg gtcgtcccga tcttctcggc atgcttgatg atgagtgcct ttgtgcagtc
    42601 tcgcatgaag ccttccggcg cccgctcgag tcgcgcctga gcttccgttg tccagtggta
    42661 gggagatgcg tctgcgcccg tattgtcctt gtcccccggg gcgacgtcgt gcgttccgtt
    42721 cccattggcc tgagcgagct tcgcctcggc agcggccgac gtgcccgcgc ccttgttggc
    42781 gaaaatcggc tgatagtctt cggaagcggc ttcctggatc atcggtgcgg cgtactccaa
    42841 ggtgatggtc gtcatgccca gcttccgagc gctcttttcg atcttcgcct tggcgcgacg
    42901 ccgctggaat cctgccggta cggcacggat ggcttctttg gcgtccttcg tccactgcat
    42961 cggcacgtcg tcataggcca gatcggtctc aacctcgccc tccgttttgg cggctgcctc
    43021 gaaaatactc ttatcaacct gcttgaaacg gtttccttcc gctccgcaca cggggcactt
    43081 gaccggtgtc tcgcctttgc caatatagcc gcagccgtcg cacacaaagt agttctgggt
    43141 catgcggtcg aggtaggcct gggtggcttc ggaggtcttg ggcttttctt cgacgacttg
    43201 cgtcatcatg ccgctgagcg tcgaacccat gaggtccttt gctacggcgt catccgcctg
    43261 catcttgtcg cgatcgatgc cagccgcgtc caggttgcca cccaatgccc gcatggcatc
    43321 catggcgccc ttcggtagaa tgtgtccgac ggccgcatcc acgaccgtgt tgctgatgat
    43381 cgtatgcccc ttttcaatcg catacctgtg aatcgcggtc ttggcaacgc cccgggcaaa
    43441 caccggaatc ttctccatcc ggcgaagcgc ttcttcagtc caggcaatgg tgtattccgc
    43501 ctgagtatcg atggggggca catatttgcg gttcgagacc aagacgttac agggagcgct
    43561 tcgcaagaga ttctccgtgt tgctgccaat gtccatgtct tcgtcgctat gtacgccgat
    43621 acgacccacg attaacaagg ccggcacgtc tttgcggaca tactggatga tcttctcgaa
    43681 ggctttgccg tccaataagg tcgtcttgac gtccgtctgc tcggcctggg cgatctcgcg
    43741 ggagatgtcc aggtgagact ggtaaatctt ggccagcccg ctatcgatga tttcctcgtg
    43801 cagcttctcc tgttccttaa accggaagac cttgcccgcc tcttcgttca gcaccccgga
    43861 tatgctgtga aacgccgcgt aatggaaata gggatcgaag gcagagatcg cctccaccgg
    43921 tatgttcaac gccttgccga gctgcaggcc cgtcatcaat ccgccgaagg agtaggggct
    43981 gccgtccact gcgacgacga tctttccccc cgtgatcggc tggatgttct tgatgatcag
    44041 catgtcggaa ttgcggacac ggcggacgac tcgttcggtg ttgctgccga tgacgctgtc
    44101 cttgaccgct cccacgccga gggcacccat gatcacgagg tcgtaggcgt tggtattgat
    44161 atcttccgcc agcaccttcc aattccgccc ttccagcgag cggcgctcaa ttggcaggtt
    44221 ggcttcattg cacttcttgt ctacgtaatc gaggtaggag tccgtaatga tctgaagccc
    44281 gcgagtaatc agcgagtcgt ggatctggcg ctgccggtcg agctccttct catcgtgata
    44341 ctcttcggga aggcccgctt ccatctgctt gaagcgcttg tcatgcatct tcgccgcata
    44401 cacgtggctt cccacaatct ttgaaccgaa cagtttcgcc aggtggaccc ccacgtccac
    44461 cgcctggttc gaatggtcgg aattatcgac cggaacatag atagtcttgt acatcgagat
    44521 gcctcctcca ggcgctctcc ggaccagggg aaactggtac aaaagttaga aaatccaacc
    44581 ggttgtcgtc ggaacctggt tggaaagagc gcgaatgata gcactgccca gaagtggaat
    44641 gcaacacgtg acgcgcaact aaccggccga agtggcgtgg gattcaggag cctgagtgga
    44701 cgaagggcgc ggacggcgtg aacgtagaat ctcctccagg gacagcagcg catcacgacc
    44761 ggaattccct tcgatgccgc ggctcaagct ttggtcggca tacgaatcat cggtctgcga
    44821 tcgttgagat agagacccgg tccatcggcg agggtctctg ctcccctgct tgcgttccca
    44881 ggcccgcagg caggccttgg caataaactt gttcagaggt gcggggttat agaggagctt
    44941 tcgaatgctc gtcggcgtca gcatcagcgg attgtagccc gcggacaaat agccttcttg
    45001 aagaagccgt tgctcgaggt cagtattcgg ctggatgccg agaaagaaca tgagcgggaa
    45061 gactcggtct tcaccaagga tcgaggccac cactctatag gactcgacgc tttgcaacag
    45121 ggattcctca gtttcctttg gactgttcag tgaatagttc aaaatcactt tcccgcgaaa
    45181 tcctgcctcg gcaaggtagc ggcagccgtc atagagccgc tcgagcttga atcccatgtg
    45241 caaggtattg agcacctctt gcgaacctga ggtgatcgcc acttccaaat cgcccacacc
    45301 ggatcgaacc atcagtttcg ccagtgcggg agtgatcaac gacgtccgga tgtagcccga
    45361 ccattcgatc tccatttttt cgctgatgat ccgttccaga atttcagtac actggggata
    45421 ggcttccttc ccggtgataa actgcgcgtc ggtgaaccag aaccgccgag ctccccactg
    45481 atgataatgc tgggagatgt cccgtacgac catttcagca ggccgatacc ggacccgttt
    45541 cccttcaatg tagggataga ggcaaaatgc acagtcatag ggacagccgc gtttcgactg
    45601 gacaccgatg gactcgccga tgtattcgcg atattgggga aaaatagacg tcagatacgg
    45661 cagatccacc gtcagggcat cgagcaaggc gggcgagccc tgcctgcctt tcttaacccg
    45721 ctttccttcc ttgataatat agcgttcatc ttcgatcgac cggccttcaa tgagtttgag
    45781 aatcgcgtct tcgccttcgc ctagcaggcc gatcgttcct tcgggcaact tctcgatcag
    45841 ctggtctgca aaggcggtga aggccccccc tccgatcatg atctgactct tggggaactc
    45901 cttccggatg agccatgggt aggacaagat cgtatagata tggctgtaat accggtatag
    45961 ctgcttgagc cctgcaaatg aggccgccac ccgcttgatc gggttgctcg caaaatagaa
    46021 attgaacgcg tgctccaagg aagaatcacc ctcgtgtggt gaaaaaatct gaatgtcccg
    46081 ccaagaaaaa cagacgaggt ccggctgaaa atcggtcatg gcgtctcgga tgaccctgct
    46141 ccgttgggaa acaggatgga gcgaaagatc aagaatgcgc tgacggactt ccggcttcct
    46201 gcggtgaaca aagtccgcca gataggtgat accgatcggg tagacctttt tgcagggaag
    46261 gaagatgtag agaatagagt tcatgcagga ccggcgaacg ttgaatgagt ggtctggtta
    46321 gcaggcggac gcatgaaggg ccttatttcc tcgcaacgtg gatcgccaca atcccgcccg
    46381 agaggttttt atagaatact tcacggaagc ccgcttcgcg caacacctgg caaagccgtt
    46441 cctggtcggg gaacgtccgg atcgacgcgg gcaaatattc atagactcca gtggtgtcgc
    46501 gggcaacttt agtgcccacc cacggcaaga gtttgaagga ataccagtca tacacggcgc
    46561 gtagccagcc aaagaccggg cgggaaaact ccagacacgc aaatgtcccg cctggtttga
    46621 tcacccggcg aatttcgctc accgcgcgtg cgaggttgcc gacattgcgc atgcagaacc
    46681 cagtggtcac cgcatcgaac gaatcgtcat cgaatcccag gtgctcagcg ttggcctgca
    46741 gacaggtgat ctttcccgtg agacccttgc cgtccacctt tttcagccct tgaacaagca
    46801 tggcatggtt gaggtccgag gcgatgaccc gcccacggac gcccatacga ggttcgagta
    46861 acaaggcaag atcggctgtg cctgctccca catcgagggc ggtgcctcgt tctacggcgg
    46921 gaatgtagga cgccgtaatc gtcttccaac gagaatgaag tccgaagctc agcagcgtat
    46981 tgttgagatc gtagaccccg gcaatggccg tgaacatgcg ttgaaccgct tgctctcgtg
    47041 cggagcccga ccaggtggaa atggcgcgtg ccgaaacgtc cggcgcctgc tgctcggtca
    47101 tggactcctc catggaacgc atgccgatgg gtggtagcac ccggtttccg gctttgtcaa
    47161 ggaacgggca gagatggcca aaacgccgcg agcttcgtct gaacgtcctg ccatcctcga
    47221 gcccagccgg caatctcgag gcgcggttgc cacaggtggt cggttcgaat gttataatcc
    47281 tgcccgtatg ggcaattcga tcgtcatcaa gggcgcacgc gaacacaatc tcaagaatat
    47341 cgacgttgag atcccccgag ataagctggt ggtcatcacg ggtttgagcg gttcgggcaa
    47401 gtcttcgctc gccttcgaca ccatctacgc ggagggacag cggcgctatg tggaatcgct
    47461 gtccgcctac gcccgccagt ttttggagca aatgggcaaa cccgacgtcg attcgatcga
    47521 aggtctctcg ccggccatct caatcgagca gaaaagcacg agccacaatc cccggtcgac
    47581 cgttgggacc gtcacggaaa tttatgatta tttgcgcttg ctgtttgccc gagtggggcg
    47641 tccctattgc ttccaatgcg gcgaggagat caccgctcaa acggttcaac agatggtcga
    47701 tgcgattggg gccctgcccg aaggaagcaa gttccaagtc ttggctccga ttgtccgagg
    47761 ccggaaaggc gaatatcgaa aagagctgtt ggagatgcgg cgggccggct atgtccgggc
    47821 gcggatcgac gggaagccgg tggatctggg cgacgacctg acgctcgata aacagaagaa
    47881 gcacaccatc gaggtcgtca tagatcgctt ggtgatgaag cccggtgatg cgttggtgcg
    47941 caggttggcc gactccgtgg aaacatctct gaagcttact ggcgggctcg tcggcgtgct
    48001 gacggaggag gggaagaccc tcttgtacag tgaaaagctc gcgtgcatca ggtgcggcgt
    48061 cagttatccg gagatcacgc cgcgcatctt ctcctttaat agtccgcacg gggcctgccc
    48121 agcctgcgac ggcatcgggt atgccatgcc tccggacgcc actgacgacc aggacctgac
    48181 actgctggaa gcctgtgagg cgtgtcatgg ggccaggctc agggccgaaa gcctctccgt
    48241 caagattgcc aggcagtcga ttgcagacgt cacgggccgg tccgtcaggg cagcagccga
    48301 attctttgcg tcgctcacat tcaccgagcg cgagacggtg attgcccata ggatcttaaa
    48361 ggaaattaga gagcggctcg gctttctggt gaacgtcgga ttggactatc tgacgctgga
    48421 ccgcgcggcg gccacgctgt ccggtggcga aggacagcgc atcaggttgg cgacgcaaat
    48481 tggctcggga ctcgtcggtg tcctctacat cctcgatgaa ccgtccatcg gacttcacca
    48541 acgggataac cggcggctgc tccaaacact gttgcgattg cgcgacctcg gaaacaccgt
    48601 cgtcgtagtc gagcacgacg ccgagacgat gctggccgct gatcacattc ttgatatggg
    48661 ccccggggca ggggcgcatg gcggccacgt gattgcacag ggcacgcctc agcagatcat
    48721 gagcgatgcc cgctccctga cagggcacta cttgcggggc gtgcagtcag tctcggtgcc
    48781 gccgcgtcac cggaaaccga agggttttct ctccgtggtc ggtgcgcata agcacaatct
    48841 gaagaatgtc acggcaaaaa ttcctttggg actcttgacc tgcgtgaccg gagtttcggg
    48901 atcgggcaag agcactctgg tgctcgaggt gctattccac tcgctctcgc agctcctcta
    48961 tcacaagaaa ccgaagatcg atggatgcat gaatcttcac ggtgtgcttg cgctggacaa
    49021 ggtgatcgac atcgatcagt cgccgattgg tcggacgccg cgttcgaacc ccgccacgta
    49081 tactggattg ttcggatacg ttcgcgatct gtactccaat ttgccggaat cgcgtgtgcg
    49141 cggatatacg ccgggtcggt acagttttaa tgtgaaaggc ggccgatgcg aagcctgcca
    49201 gggcgacgga ttgatcaaga ttgaaatgca ttttttaccc gatgtgtacg tcacttgtga
    49261 ggtgtgcaaa gggcaacgct acaaccgcga gacgctggag atcctttaca aggacaaaag
    49321 tattgccgat gtgctgaaca tgacggtgga agaagcgctc gcatttttcg agaatatccc
    49381 gttggtcaag agcaaattgc agacactgca tgacgtcggt ctgcattatg tcaagcttgg
    49441 gcagtcggcg accaccctct ccgggggaga ggctcagcga gtaaaattat ctcgcgagtt
    49501 gtccaagcgc gcgaccggac ggacgctgta cattctcgac gaacccacaa ccggcctgca
    49561 cttcgccgat gtgcagcggt tgctcgatgt cttggatcgg ctcgtcgaag c
//
LOCUS       DASSCQ010000088         3622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1227886, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000088 DASSCQ010000000
VERSION     DASSCQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227886"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFH05_06350"
     CDS             <1..444
                     /locus_tag="VFH05_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6677868.1"
                     /translation="DPRGDQRTGGWWMAGALVSMPLFTGFLIENQVVEATAQKEAAAA
                     ATLNVEQALTQQVTNAYLDTVTFSQQIKLGEEQVRTAQEALQLAKQRYRLGLGSVVEV
                     TQSEVALTLAQTRLAEAQYDYKISEVTLAYTSGGSAQLQVDPTIR"
     gene            complement(466..1212)
                     /locus_tag="VFH05_06355"
     CDS             complement(466..1212)
                     /locus_tag="VFH05_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677869.1"
                     /translation="MSRKSGRERTWRRLVRLVVLSAFLPLPALLLFAGCAIPHVPYRT
                     IYEDPVNYVRLEEDDAVLPEWPPGHHAHPKTFTAGEMKVLLKGMTIQEHRIWLQKWLQ
                     GDAPFVPAFKDEETELLASQLIEAFAAANYNERVTFYLSQPQTSVKRVITSGGMYVHG
                     DELHVILGNWQIVYGIPTYGMIYDRRYPMRPTAAKGFDLFFQPSNAVVSQRSSVWDTL
                     FANTKDELVIDMIRWQGAVTSYSARTHPPE"
     gene            complement(1230..1739)
                     /locus_tag="VFH05_06360"
     CDS             complement(1230..1739)
                     /locus_tag="VFH05_06360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6677870.1"
                     /translation="MAEVTDKTRATISVTSKGQAVGDIVVRFFHDVAPGHVKNFVDLA
                     TKGFYNGTTFHRVIPKFMIQGGDPNSKNADRSSHGMGGPGYKIKAEFNSKPHKRGVVS
                     MARANDPDSAGSQFFICVADSHFLDWQYTAFGEVVSGMDVADTVVNMKRDGRDNPLER
                     VEMTISISE"
     gene            complement(1819..2844)
                     /locus_tag="VFH05_06365"
     CDS             complement(1819..2844)
                     /locus_tag="VFH05_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677871.1"
                     /translation="MMNRSLLSLAMTLLTLSLVGSAVAQHPSSSPFESQAASIQALTM
                     VGSDILYAGSFGLGVFRSEDRGATWTKSSEGVSDPFILSLATAKDGTVYVGTFRGGVF
                     RTTDQGKTWHPVNAGLKRKEVKTLLAVGDLLYAGTADGAYRLNSTDDHWGVVTSGLDE
                     ILVHALAQSADGTLYAGTSGKGVLKFKANTTGWVRLHHGLKDHEGMVENFIRVLTIDR
                     EGGIYAGTFDGGVFRSPDGGNTWRPISRALPNDSIRGILFNSRGLFVATGHGIFKTTD
                     KGRQWVPLNNGLSSMAVQVLVESPSGSLYAGTNAGAFRSDDDGKSWSPINEGLEGGVA
                     PPPFLFR"
     gene            complement(2853..>3622)
                     /gene="tatC"
                     /locus_tag="VFH05_06370"
     CDS             complement(2853..>3622)
                     /gene="tatC"
                     /locus_tag="VFH05_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET6677872.1"
                     /translation="PMTNPLAAHIQTIKRRLLIIAATVLVFLMVAFAFSAEMVAWLNR
                     PFPNPLVFYGPTEALFASIKVSFLAAVLASLPVVFYQLWKFVQPALLPKEQRWGIPIL
                     ALAGVLFALGLLFCNLVIRPLVIDFFVSFGMDRDITPELSVGTYIDFNVKFLLTFGCA
                     FELPLVLTILARVGVVTAGQLAHYRKHAILSALIISAVVTPDATLFTMMLMAVPLMVL
                     YEIGILGARLFGRNQPPDMNLPLDPDLPMGTAGHRVR"
BASE COUNT          817 a         1056 c         1039 g          710 t
ORIGIN      
        1 gacccacggg gtgaccaacg aaccggtggg tggtggatgg caggagcgct cgtctccatg
       61 ccgttgttta ccggctttct cattgagaac caggtcgtcg aggctacggc tcagaaagaa
      121 gccgcagccg ccgcgaccct caacgtggag caggcgctca cacagcaagt cacgaacgcg
      181 tatctggaca ccgtcacctt ctcgcagcag attaaactcg gtgaagagca ggtcagaaca
      241 gcccaggaag cactccagct ggcaaaacaa cgatacagac tagggctggg atcggtggtg
      301 gaggtgacgc agtcagaagt ggcgttgacc ttagcacaaa cgcgcctggc cgaggcgcag
      361 tacgactata agatttccga ggtcaccctg gcctacacct ccggaggcag cgcacagtta
      421 caggtcgatc cgactatacg gtagacacgc atcccgagca gatctttact ccggaggatg
      481 ggtgcgggcg gaataggacg tgactgctcc ctgccagcgg atcatgtcga tgacaagctc
      541 gtccttggtg ttggcgaaga gtgtgtccca gacgctactc cgctgcgaca ccactgcgtt
      601 cgacggctga aaaaagaggt caaaaccctt ggctgccgtg gggcgcatcg ggtaacggcg
      661 gtcgtaaatc atgccatacg tggggatccc atacacgatt tgccaattac cgagaatcac
      721 gtgcaattcg tcgccatgca cgtacatgcc tcccgaagtg atcacgcgct tcaccgaggt
      781 ttgaggctgg ctcaggtaaa acgtcacccg ctcgttgtaa ttggccgccg caaaggcctc
      841 gatcaactgg gacgccagaa gctccgtctc ttcgtctttg aaggccggca caaaaggcgc
      901 atcgccttgc agccattttt gaagccaaat gcgatgttct tgaatggtca tccctttgag
      961 cagcaccttc atctccccgg cggtgaacgt cttgggatgc gcatggtgcc ccggaggcca
     1021 ttcggggagt accgcatcat cttcttccaa ccggacgtaa ttgacgggat cctcatagat
     1081 ggttcggtag ggtacgtggg gaatcgcgca accggcgaag agaagtagcg ccggaagcgg
     1141 aaggaaggcc gacagtacca caagccgtac caggcgccgc cacgtgcgtt cccttccgga
     1201 cttgcgactc atcgatcgac actcacacgt cattcactga tgctgatcgt catctcgacg
     1261 cgctcgagag ggttatcgcg gccatcacgc ttcatgttca cgaccgtatc ggccacgtcc
     1321 atcccgctca ccacttcgcc gaaggcggta tattgccaat ccaggaaatg tgaatcggcc
     1381 acacaaatga aaaactgcga gccggcgctg tcgggatcgt tggcacgtgc catcgagacc
     1441 actccccgct tgtgcggctt gctgttgaat tcggctttga tcttatatcc aggtccaccc
     1501 atgccgtgag aagagcggtc ggcattcttg ctattagggt cgcccccctg aatcatgaac
     1561 ttgggaatca cgcgatggaa cgtcgtgccg ttgtagaaac cttttgtggc taggtcgacg
     1621 aaattcttga cgtggcctgg tgcgacgtca tgaaagaatc tcaccactat atccccgacc
     1681 gcttgtccct tgctggtcac agaaatcgtt gccctggtct tatcagtcac ctcagccatc
     1741 tcgccgttcc tttcttgttg gctctgatca atcgctctgc tgacgccagt tgagaacgca
     1801 ggcggctccg ctccagcact agcggaagag gaagggcggc ggagccacgc cgccctctag
     1861 cccttcgttg atcgggctcc agctcttgcc atcgtcgtcg ctgcggaatg cgccggcatt
     1921 cgtccccgca tacaatgatc cggaaggaga ctcaaccagc acctgcacgg ccatactgct
     1981 cagtccatta ttcagcggca cccactgccg gcccttgtcg gtcgtcttga aaatgccgtg
     2041 ccccgtcgca acgaacaggc cacggctgtt gaataggatg ccgcgtatgg aatcgttcgg
     2101 caaggcacgg ctgataggac gccaggtatt gccgccgtct gggcttcgga agacgccgcc
     2161 gtcaaaggtc cctgcataga tccccccttc gcggtcgatt gtaaggaccc gaataaaatt
     2221 ctccaccata ccttcgtgat ccttcagccc gtggtgaagg cgcacccagc cggtcgtgtt
     2281 ggctttgaat ttcaggactc ccttcccgga cgtcccggca tagagcgtgc cgtcggccga
     2341 ttgtgctaag gcatgaacca gaatttcatc gaggccgctc gtcacgactc cccaatggtc
     2401 gtcggtcgaa ttcaaacgat acgcgccgtc tgcagtcccc gcatacagca gatcgcccac
     2461 cgccaacaac gtcttcactt ctttgcgctt caatccggcg ttgactggat gccaggtctt
     2521 gccttgatcc gtggttcgga acactcctcc ccgaaaagtt cccacataca ccgttccgtc
     2581 ttttgccgtt gcgagcgaca atatgaacgg gtcactcacc ccttcactgg atttcgtcca
     2641 ggtcgctccg cgatcctcgc ttcgaaagac gccaagccca aatgagccgg catagaggat
     2701 atccgaaccc accatcgtga gcgcctgaat actggccgct tgcgattcga aggggctcga
     2761 tgatgggtgc tgagcgacgg cggaaccgac cagcgacagg gtcaggaggg tcatggcaag
     2821 agaaagcaat gatcggttca tcatgacgta tcttaccgaa cccgatggcc cgctgtcccc
     2881 atgggtaggt caggatccag tggaagattc atgtctggag gctggttgcg gccgaacaga
     2941 cgggcgccga ggatgccaat ttcatagagg accatcaagg gcacggccat gagcatcatg
     3001 gtgaagagcg tggcatctgg ggtcaccaca gcggagatga tcaaggcgct caagatggcg
     3061 tgcttgcgat agtgagccaa ctgccccgcc gtgacgacgc cgacacgagc gagaatggtc
     3121 aagacgaggg gcaattcgaa cgcacatcca aaggtcagca gaaatttgac attaaaatca
     3181 atgtacgtgc cgacgctcag ctccggcgta atgtcgcggt ccatgccgaa gctgacgaaa
     3241 aagtcgatga cgaggggacg gatgacaagg ttgcaaaaaa gaaggccgag ggcaaacagc
     3301 accccggcca gcgcaagaat ggggatgccc cagcgctgct ccttcgggag cagcgctggt
     3361 tggacgaact tccacaactg ataaaagacg acaggcaggc tagccagcac cgccgcaagg
     3421 aaagaaactt tgatggaagc gaacaaggct tccgtcgggc cgtagaagac cagtgggttg
     3481 ggaaacgggc ggttcaacca ggccaccatc tcggctgaaa aggcaaaggc caccatgagg
     3541 aacacgagca cggtggctgc gataatgagc aggcgccgtt tgatggtctg tatgtgagcg
     3601 gccagcggat tggtcatcgg cg
//
LOCUS       DASSCQ010000089         6774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1839094, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000089 DASSCQ010000000
VERSION     DASSCQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839094"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2788
                     /locus_tag="VFH05_06375"
     CDS             <1..2788
                     /locus_tag="VFH05_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET6677873.1"
                     /translation="MMAAKERLPEGVEPVMAPVSTGLGEVYQYYLEGPHAKDSDEAVV
                     QAELTEQRTLQDWVLRPLFKGIPGVIDVNVLGGFVKQYQVLVDPSKLRKYDLTLHEIY
                     DAVAKNNANAGGNVLERHAERSIVRGLGLIKTLADIEAIIVKEVEGTPIFVRDVAEVR
                     IGHAVRHGAAVLNGEREVVAGTILMLRGGNARRVVDAVSAKVNELHQMNVLPPGTTIV
                     PFYDRIELVRAAVHTVRDALIEGVVLVVLVFFFFLGHVRSAIIVTVTLIVTPLVAFIL
                     MERLGLSANLMTLGGLAIAIGEIADGSLVVVENVYRHLGNHRAGQRTTLEVILEATKE
                     VGRPILFGTLIISVVFLPLMTLHGMEGKMFAPLAYTLVIALLASVLVTLTLSPVLASL
                     LLRGDHPEETRLTRWMKQAYSPLLHWTLGHRRHVLIGSTVLVLASLFLLPRIGREFIP
                     ILEEGALTPQIVRLPSVSLSESIEMEKQTHKVMLEFPEVRMAVSKIGRPDIAFGPEEP
                     NESDPIVSLHDRSTWTTAQTQSGLTDAIRRKLAEIPGISVLMSQPIQERVDELISGIR
                     TECAIKLFGPDLDVLHDKAEEIAMTMQDVEGVKDIKVEQIAGQPYLTIDIDRQKIARY
                     GINVADVQELIVTAIGGKSATYVYEGERRFQLVLRFPEPARNSVGTIGEIRVKAASGA
                     LIPMHDLATIEMRQGPLRISREDVKRRIYIGFNVVGRDIGGVVDEARKKLEAQIRLPQ
                     GYTISWGGAFENMERANARLLIVVPITLGLVFFLLFWAFHSLRYATLIILNLPFALVG
                     GVVSLWLSGQYLSVPASIGFIELFGLAVGNGIVLVSYINQLRRDGMASDKAIVTGCSL
                     RLRPVVMTMMTTLLGLLPLALAQGIGAEVQRPLATVVIGGIFSSTALTLIVLPALYHL
                     FAEKVETTFTPEWV"
     gene            2807..5494
                     /locus_tag="VFH05_06380"
     CDS             2807..5494
                     /locus_tag="VFH05_06380"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET6677874.1"
                     /translation="MTQITTTPSPSWHALSPQEVAAALSTDLKDGLSGDEAQRRQTHD
                     GFNELPEAPPPSIVKLFLSQFTSVIIWVLLGAAIVSGLLEDWLDAAAIVAIVFLNGVL
                     GFIQEFRAERSLAALRKMSVAMARVIRGGNLESIPARELVPGDLLLLESGDRIAADAR
                     LIYTVGFQTQEASLTGESTPVQKEAREVVPENAPLADRTNMAFMATIAVSGKSHALVV
                     ATGLNTELGRIAGMIQQATAAEKMETPLQRRLEQFGYTLLWLALAVVAIVFGLGYWRG
                     EPLLEMFLTSVSLAVAAVPESLPAVVTITLALGVTRMAKRHALIRKLPAVETLGSATV
                     ICTDKTGTLTKNEMTVTTLVVGEETFGVDGEGSPLPSHAPGTKQLLTAAVLCNGAELH
                     EQNGVRRVIGDPTEGALLVLAERFGMAKQALEREAPFESEIPFNAERKMMTIIRRSPA
                     GNMAYVKGAPDVLLPRCTHRVSLDGHVEPLDERHRQEILATNGALAGQALRVLAVAHR
                     LLNAPQGKMQASTIEQELVFLGLVAMKDPLRPEAVEAVRLCREAGITTTMITGDHKDT
                     AVAIARELGLYQGNSSALSAHDLDKLTDEQLAQRVESIPVYARVSAEHKLRIVQAWKR
                     RGAVVAMTGDGVNDAPAIKAADIGVAMGITGTDVTKEAADMVVTDDNFASIVAAVEEG
                     RAVFDNIRKTVHFLLSCNVSEVLVMLFSTLLGLPLPLLPIQILWMNLVTDGIPALALA
                     VDPKAPDLMKRRPRRPDARLLDGKRLVAIGLEGLMLSVIALGAFVYSLYGWHQTVEQA
                     RTVAFSVMVVAQLVHAFNCRSERLSLFQVGLFTNRALVWAFLLSFVMQVAVLTVPTAS
                     AIFKVSPLSLGNWELMAAMVLLPLILVEAAKWNKRLSST"
     gene            5908..6351
                     /locus_tag="VFH05_06385"
     CDS             5908..6351
                     /locus_tag="VFH05_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6677875.1"
                     /translation="MLTKTTRGKKSGKDFAFLTVKDVMKKEVRFAHLKTKGDVIASLM
                     IAGFGAVPVVNGGQRLAGIVSEHDLLAAIDDGHELSRVTAADIMTGNPYSVRLETTLG
                     TLVHVLRASDLVRVPVVDANNKLVGIIARRDLLRTYVGGNSSKRK"
     gene            6361..>6774
                     /locus_tag="VFH05_06390"
     CDS             6361..>6774
                     /locus_tag="VFH05_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6677876.1"
                     /translation="MRQTEFYLSACDPKTLTVGQLMQDAVTTCTPRTDVSTIASMMTR
                     RNLGSLPVVEQDGTLVGIVTEYDLLQAMIEGRDLRNISAMGIMSTVPVTVTEDRTLVQ
                     VAELFQDRYITRVPVVRNKKLVGILARRDLLFGYLK"
BASE COUNT         1359 a         1883 c         2105 g         1427 t
ORIGIN      
        1 gatgatggca gcgaaagagc gcctacccga aggcgtcgaa ccggtgatgg caccggtgag
       61 caccgggctc ggcgaagtct atcaatacta tctggaagga ccgcacgcga aagactctga
      121 tgaggccgtc gtgcaagccg agttgacaga gcaacggact cttcaagact gggtgctgcg
      181 acctctattc aagggcattc ccggcgtgat cgacgtgaat gtgttgggcg gattcgtgaa
      241 acagtaccag gtgctcgtag acccgtcaaa gctgagaaag tacgatctga cgctgcatga
      301 gatttacgac gccgttgcga agaacaatgc gaatgcgggt gggaatgtcc ttgaacgtca
      361 tgcggaacgg tccattgttc gcggtttggg tttgatcaaa acgctcgccg atattgaggc
      421 catcattgtg aaggaggtgg agggcacgcc tatctttgtc cgcgacgtgg cggaagtccg
      481 tatcggccat gccgttcggc acggcgccgc tgtcctcaac ggagagcgcg aagtggttgc
      541 cgggaccatt ctcatgctgc gtggcgggaa tgcgcgtcgc gtggtggacg cggtgtccgc
      601 caaggtgaac gaactgcacc agatgaatgt gctgccgccg ggcacaacga tcgtgccctt
      661 ttacgaccgg atcgaactgg tccgggcggc tgtccatacc gtccgggacg ccttgatcga
      721 aggcgttgtg ttggtagtcc tcgtcttctt tttcttcctc ggtcatgtgc gcagcgcgat
      781 catcgtcacc gtgacattga tcgtgacgcc attagtcgca ttcatcctca tggagcgact
      841 gggactctcg gccaatctga tgacgcttgg cggcctggcc atcgccattg gcgagattgc
      901 tgatggttcc ctggtggtcg tggagaatgt ctatcgccac ctcggaaatc accgcgctgg
      961 tcaacgcaca actctcgagg tcattcttga ggcgacgaaa gaagtcgggc gtccgatcct
     1021 gttcggcact ctgatcatca gcgtggtgtt cttgccgctc atgacgctcc atggcatgga
     1081 aggaaaaatg ttcgccccgt tggcctacac cctcgtcatt gccctcctgg catcagtact
     1141 ggtgaccctg actctctcgc cggtgctggc ctcgcttctg ctccgcggcg atcatccgga
     1201 ggaaacccgg ctgactcggt ggatgaaaca ggcgtactcg ccgttgctgc actggaccct
     1261 cggccatcgt cgtcacgtgc tcatcggctc gaccgtattg gtactcgcaa gcctgttcct
     1321 cctgccccgg atcgggcgcg aattcattcc catactggaa gaaggggcct tgacccctca
     1381 aattgtccgc ctccccagcg tatccctttc cgagtcgatc gaaatggaaa aacagaccca
     1441 caaggtcatg ctggagtttc cggaggtgcg catggccgtg agcaagatcg ggcgtcctga
     1501 catcgcgttc ggaccggagg aaccaaacga gagcgacccc atcgtgtcct tgcacgatcg
     1561 tagtacatgg acaacggcac agacgcaatc ggggttgacg gatgccatcc gacggaaatt
     1621 ggcggaaatc cctggcattt ccgtgctcat gagtcagccg atccaggaac gagtggacga
     1681 actgatttcc ggcattcgta cagaatgcgc catcaagctg tttgggccgg acctcgacgt
     1741 gctgcatgac aaggcggaag aaatcgcgat gaccatgcag gacgttgaag gcgtcaaaga
     1801 catcaaggtg gaacaaatcg ccggccagcc gtacctcacg atcgatatcg accggcagaa
     1861 gattgcccgt tacggcatca atgtggccga cgtgcaggaa cttattgtca cggccatcgg
     1921 ggggaaatct gccacctacg tgtatgaggg cgagcggaga ttccagctgg tcctgcgttt
     1981 tccggagccg gcccgaaaca gcgtggggac catcggagag atccgcgtga aagcggcatc
     2041 cggcgcactc attcccatgc acgatctggc cacgattgaa atgcggcaag gcccgcttcg
     2101 catcagccgt gaggatgtga agcgccgcat ctatatcgga ttcaacgtcg tgggccgtga
     2161 catcggcggc gtcgtcgatg aagcgagaaa gaagctggag gcacagatcc gcttgccgca
     2221 gggatacacc atctcgtggg gtggcgcctt tgaaaacatg gaacgtgcga acgcgcgact
     2281 cttgatcgtg gtaccaatca cgctggggct cgtcttcttt ctcctctttt gggcgttcca
     2341 ttcgttgcga tacgcgacgc tgatcatcct caacctgccg tttgccctgg tcggcggggt
     2401 cgtgtcactc tggctgagcg ggcagtactt aagcgtcccg gcctccatcg gcttcatcga
     2461 actattcggc cttgccgtcg ggaacgggat tgtgctggtg tcctacatca atcaactgcg
     2521 gcgggacggg atggcgagcg acaaggcgat cgtgaccggt tgcagcctgc gactgcgtcc
     2581 cgtcgtcatg acgatgatga cgaccttgct gggtcttctg ccgcttgcgc tcgcccaggg
     2641 aattggagcg gaagtgcagc ggccgctcgc tactgtggtc atcgggggta tcttcagttc
     2701 aaccgcgctc accctgatcg tactgccggc gctctatcac ctctttgcag agaaagtcga
     2761 gacgacgttt acacccgaat gggtgtgaag gcgtggggcg gcgtttatga ctcagataac
     2821 cacgacaccc tcaccttcat ggcacgcgct gtcaccacag gaagtggcgg cagcactctc
     2881 caccgacctc aaggacggcc tcagcggtga tgaggcgcaa cgccgccaaa cgcatgacgg
     2941 attcaacgaa ctccccgaag ctccgccgcc ttccatcgtg aagttgtttc tgtcccaatt
     3001 caccagcgtc atcatctggg tgttgttggg ggccgccatc gtgtcgggct tgctggagga
     3061 ctggctcgac gcggcggcca tcgtggccat tgtttttctc aacggtgtgc ttgggtttat
     3121 tcaagagttc cgggccgaac ggtctctcgc tgccctgcgc aagatgtcgg tggcgatggc
     3181 gcgggtgatc cgagggggga acctcgagtc aattccggct cgcgagttgg tgcccggcga
     3241 tctgcttttg ctcgaatcag gggatcgcat cgccgccgat gcccgcctga tctataccgt
     3301 tggattccag acgcaggaag cctccctgac cggcgaatcc accccggtac agaaagaggc
     3361 acgggaagtc gtgcccgaga acgcgccctt ggcggaccgg acgaatatgg cgttcatggc
     3421 caccattgcg gtgtcgggga agtcccacgc gctcgtggtc gcgaccgggc tgaacaccga
     3481 actgggtcgg atcgccggca tgattcagca ggcgacggcg gcggagaaga tggaaactcc
     3541 actgcagcgc cgtctggagc agttcggcta tacgctgctg tggctggcgt tagccgttgt
     3601 ggcgatcgtg ttcggcttgg gctattggcg gggcgagccg ttgctggaga tgtttctgac
     3661 ctccgtcagc ctggcggtcg ccgcggtgcc ggaaagcctt cccgcggtgg tcaccatcac
     3721 gctggccctc ggggtcactc gtatggcgaa gcgccatgcg ctgattcgga aactgccggc
     3781 cgtggagacg ctcggttccg ccacggtgat ctgcacggat aagaccggaa ccttaacgaa
     3841 aaacgaaatg acggtcacga cacttgtcgt cggagaggaa acgtttgggg ttgacggtga
     3901 aggatctcca cttccaagcc atgctcctgg gacgaaacaa ctgcttaccg ccgctgtgct
     3961 gtgcaacgga gcggagctgc atgaacagaa cggagttcgg cgggtcatcg gtgatccgac
     4021 cgaaggagcc ctgctcgtat tggccgaacg attcggcatg gccaaacagg cgctggagcg
     4081 ggaggcgccg ttcgagagcg aaattccttt caatgccgag cggaaaatga tgaccatcat
     4141 ccggcgatcg ccggccggga atatggcgta tgtcaagggc gcgccggatg tcctgcttcc
     4201 tcgttgcacc catcgcgtca gcctcgacgg ccacgttgag cccttggacg aacggcatcg
     4261 acaggagata cttgcgacca acggggcatt ggcaggacag gcgcttcgcg tcctcgccgt
     4321 ggcgcatcgc ttgttgaacg cccctcaggg aaaaatgcag gcttcgacca tcgagcaaga
     4381 actggtgttt ctcggtctgg tggccatgaa ggatcccttg cggccggaag cggtggaggc
     4441 ggtgcgattg tgcagggagg cgggcatcac cacgacaatg attaccggcg atcataaaga
     4501 cacggcggtc gcgattgcca gggagctcgg gctctatcag gggaacagct cagcgctctc
     4561 tgcgcatgac cttgacaagc ttacggacga gcagctggcc cagagggttg agagcatccc
     4621 ggtctacgcc agggtctctg ccgagcataa gctgcgtatc gtgcaggcct ggaagcgccg
     4681 gggcgcggtt gtcgcgatga ccggcgacgg ggtgaatgat gcgccggcca tcaaagcggc
     4741 ggacatcggg gtggccatgg gcataacggg gaccgatgtg accaaggaag ccgccgacat
     4801 ggtcgtaacc gatgacaact tcgcttcgat tgtggctgcg gttgaggaag gccgtgcggt
     4861 cttcgacaac attaggaaga ctgtgcattt tttgctgtcc tgcaatgtca gcgaagtgct
     4921 ggtgatgcta ttttccacgc tcctcggcct tccgcttcca ctcctgccga tccaaattct
     4981 ctggatgaat ctggtgaccg acggaattcc agccttggcg ttggccgtcg accccaaggc
     5041 gccggatttg atgaagcgcc gccctcgccg tcctgatgct cgattattgg acggcaagcg
     5101 attagtggcg atcggcctcg aaggattgat gctgagtgtc atcgctcttg gggcgttcgt
     5161 gtatagcctc tacggttggc accaaacggt ggagcaggcg agaacggtgg cgtttagcgt
     5221 catggttgtt gctcagctgg tgcatgcgtt caattgtcgg agcgagcggc tgtcgctctt
     5281 tcaagtggga ctcttcacga atcgggccct ggtctgggcc tttctgcttt cattcgtgat
     5341 gcaggtcgct gtactgaccg tccccacggc ctcggcgatc tttaaagtct caccattgtc
     5401 gcttgggaat tgggaattga tggccgccat ggtgcttctg cccctgatcc tcgtggaagc
     5461 ggcaaaatgg aataagcgtc tgtcgagcac atgagcagtc gattttcgcg agcccccgcc
     5521 cccaagttga acgaatcggt tcactcggcc tgcacagatg attctcacga gtcccttgca
     5581 tccactcttc ttttcttcca tagcatggtg gttgcattcg atgtgcctgg ttggccgaat
     5641 tggacgagaa gaacgactta attccaggtt accgcatgca tgacccgttt agggacgtgg
     5701 agatgccgcg ttttgcagca ctcagccaga aggcactggg gtgattggcg tcagttatgc
     5761 gtcggtgacg ggctggtcta gcggacagcg gcttcccagc gagggccttg gaaccgccgg
     5821 ggcggaattc ttgtaaccga cgagaccgtc taagcgtacc ttcctctcat gagaggaggt
     5881 ccatctctta gcgcataagg aggagagatg ctgacgaaaa caacacgggg caagaagtca
     5941 ggcaaggact tcgccttttt gacagtgaag gacgtgatga agaaggaggt gcggtttgcg
     6001 catcttaaaa caaagggtga tgtgattgca tcgcttatga tcgcagggtt cggggcggtc
     6061 ccagtggtca atggtggaca aaggttggct ggcatcgtca gcgagcacga cctccttgca
     6121 gccattgatg acggtcatga gttgagccgg gtcacggcag ccgacatcat gaccgggaat
     6181 ccctattcag tgaggctcga gacgacgctg gggacgctcg tgcatgtact gcgcgccagc
     6241 gacctcgtcc gcgtgcccgt tgtggatgcc aataacaaac tggtgggtat catcgcccgg
     6301 cgcgatctgt tgcgcaccta cgtgggcggc aattcttcaa aaagaaagtg aggaacagcg
     6361 atgcgacaaa cagagtttta cctgagcgcc tgtgatccga aaaccctgac ggtcggacaa
     6421 ctcatgcagg atgccgtcac gacctgcacc ccacgcactg acgtctcgac aattgccagc
     6481 atgatgactc gtcgcaatct cggcagtctt cctgttgtgg agcaggacgg gaccctggtg
     6541 ggaatcgtga ctgaatacga cctcctgcag gcaatgatag aaggaagaga tcttcggaac
     6601 atatccgcaa tgggaatcat gtctaccgtt ccagtgacgg tgacagagga ccgaaccctt
     6661 gtacaggtgg ccgagctttt ccaagaccgg tatatcacac gggtccccgt ggttcgaaac
     6721 aagaaactcg taggcatact ggctcgacgc gatctgctgt ttggctatct gaag
//
LOCUS       DASSCQ010000090        10610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1667360, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000090 DASSCQ010000000
VERSION     DASSCQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667360"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1099)
                     /locus_tag="VFH05_06395"
     CDS             complement(38..1099)
                     /locus_tag="VFH05_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677877.1"
                     /translation="MPPKPVQTPVFQADVTPTINGSPVPPTSVRNLPWSAERPGVNGL
                     LASTTWLKGAFATETEVAANQTILPGADRSTRVTRFAVTASAGLVRYGMRIRDAGQGF
                     NHQADQGVREAWSEWSIGTMALRSAIGQQWTYLQGDAAQDRAKQSYNRIDVSWRKGAW
                     PHLAFSYAQNTASNTMDPLNFYPQRASPHTLEAAVGYSGGIWDAKLASVYGIEADLLN
                     HGAESQVRTQTMTASLRPIRPLTITPSLGYRAERPEWSSARIDAPSASLTMSYKQSHR
                     LSITAVGNYFGLRSTDKLIDLDNIGGKGIVSWELEPLRDWKPQLSVEGGYNLQVNRLM
                     PSAQTENISGLLRLVLATM"
     gene            complement(1287..1700)
                     /locus_tag="VFH05_06400"
     CDS             complement(1287..1700)
                     /locus_tag="VFH05_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677878.1"
                     /translation="MMYGLKSASAIVLLSIAGCAMTSKAPDFSGLKDVDGSDAVHINM
                     TKAALHFAVALPFIGDASLEGAVRDFTEEAKKEGAERVRIVQSDETTLWWILPPVSFL
                     FTPKFTNVAGEALLAFNDTTPQKHTEAEAEKRVKE"
     gene            complement(2085..3281)
                     /locus_tag="VFH05_06405"
     CDS             complement(2085..3281)
                     /locus_tag="VFH05_06405"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HET6677879.1"
                     /translation="MSRFYRAGVVVVLALVVLACNNGDDSSSGTPNQPPSFNNTVLML
                     APPADGSGDVYVGGDFTTYDGAQAIRIVRLNGDGTIDPGFATGLGFNGSVRSIVPAGT
                     QGATIYVGGDFTTYNGIQRSRIVRLSANGEFDPTFAIGTGFDNTVHVIASAGDGTGDI
                     YVGGAFTNYNGITANHLVRLNADGTVDSTFIMGPGFNNPVFTIMPTTDGSGDLYVGGA
                     FTSYKQVLANNLVRLNEDGSLDATFLTGTGFDNAVQTIAPADNGGLYAGGAFTNYNGT
                     PANGLVRLNSNGVINPFLSTGKGFNNTVFHVVPAGDASGDLYVGGSFTTYNDMQANQL
                     VRLNQNGTIDLTFATATGFGNPVLRVLPVGDASGDVYAGGQFVQYQSTSIGRFVRLTS
                     TGAFLR"
     gene            3944..5017
                     /locus_tag="VFH05_06410"
     CDS             3944..5017
                     /locus_tag="VFH05_06410"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET6677880.1"
                     /translation="MLPQIAWKAVLEHNSRFDEAFVYAVASTRVYCRPSCPSRRPDRR
                     RVTFFASPEQAEAAGFRACLRCAPGSPRGTQTERLVEQVRQYLDGHSDEPATLTDLAA
                     LVGMSPFRLQRAFTRVFGFSPKTYQNAKRIQRFRQALERGVGITDASYQAGFGSSSRA
                     WEQASRHMGMTPSAFRSGGKGVMMHYASLRTPVGFLLIAATERGLSSVMLGGSEPLLV
                     AKLKRQYPNAQLRRNPKRLLPYRQALARCLNGNPAGAGLTLDVKPTAFQFQTWMALQQ
                     IPAGSTRTYREIACTIGRPAASRAVARACATNPLALLIPCHRAVGQDGRLAGYRWGIR
                     RKQQLLRLEAARRQHGRPSKRSS"
     gene            complement(5014..5769)
                     /locus_tag="VFH05_06415"
     CDS             complement(5014..5769)
                     /locus_tag="VFH05_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6677881.1"
                     /translation="MPKRVRTGLTRTDILHRYLERFKQQLIKLRPFLSRKRSTTTIED
                     FDESAEELISQVFGAASDEAEAYFYAKNGESALLPEEAQENGTHNIERESLQQRRQVL
                     ESCMADLEMRRLIQSAKQSNGKGMLQAKVEDYMSHDVRSIHRAATIKEAGLLLQKYKV
                     GSLIVDDGSRYIGIVTDSDLSRKAVAKGLDPNTTTVTTCMSKSLVTIEEDELLSEAMN
                     LMKKQGIRHLPVTADGTIIGVLSLADLLRAFMD"
     gene            complement(5863..7287)
                     /locus_tag="VFH05_06420"
     CDS             complement(5863..7287)
                     /locus_tag="VFH05_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET6677882.1"
                     /translation="MDELEESGHDDRHMAASNGKQNFARYMIGGLCLILFLALTGVGY
                     VQVEERRGGGLRPFVSAENKKCIDCHMAKDVAVGGINDWKVSRHAPKGIGCVECHRAE
                     KGEADGYDHEGFLISTLVTPKDCMRCHDREAKEFAKSHHAKAAQFTGSLDNFLGNVVE
                     GPEVVTTGCAGCHGSVVKVMEKGKLHPATWPNSGIGRVNPDGSKGTCSACHARHSFSI
                     AQARQPESCGRCHMGPDHPQIEAYYESKHGVMYEANKEKMKLADPTEKWHPGKDYLYP
                     TCATCHMSATGTQEVTHDVGDRISWTLRPVISTRLENYEDRRKSMKQICSACHSEQIV
                     DRFFTSMDQGINLYNDKFGKPAKEAMDKLLAMKKLTPTPYDEKIEWVFYELWHHEGRR
                     ARHGLSKVAPDYVHWQGFYEVAKSFYTKFLPLVRELSPQVAEELLRNESHRWIEKGMT
                     PEEIAQMLEFYNKEMQGKRGGVDG"
     gene            complement(7302..8330)
                     /locus_tag="VFH05_06425"
     CDS             complement(7302..8330)
                     /locus_tag="VFH05_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET6677883.1"
                     /translation="MDSTTTQHIDPKGTGKTSWIEYIQKDLPQHLDWWPYTLGAIPLT
                     LFGILVATGLLLTFYYVPSPEKAYESVDQITNEIYLGWFVRGLHKTSVDLMILFLLFH
                     VIRVFITRAYRTPGELKWVSGSIVLFVTLAMGFTGYSLVFDNVSYWGMTVVTNMVGTL
                     PVIGTPLLHLLRGGEDVSGVTLLRLYDLHTKLLPVLLGGLVLGHVLIVRLLGFADVRG
                     SRGFHPFYPEHTLKMGVVAVGLLLLIVNIAMIFPPLLGPPANPQEVASDVSPPWYFSA
                     PFMWITLLPGPVALWSLLGFAGLFVIYPFLDRMLAERGWPMDVVNAIAAAAVVGAMVF
                     LMYLDTQM"
     gene            complement(8336..8782)
                     /locus_tag="VFH05_06430"
     CDS             complement(8336..8782)
                     /locus_tag="VFH05_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET6677884.1"
                     /translation="MAIVHDDAGRRRFLSQAVMGFGMLFGLGTLGFRFMQFLLPTRTD
                     KQPETVLIGAETRIPLGDAVPMDLGGHKILVLKTQDGVAAFSRRCTDLGCLVSWNKER
                     EQFVCPCHQGTFDKNGVNIAGPPPRPLDRFQIVKRGEQLYVNIASA"
     gene            complement(8785..9945)
                     /locus_tag="VFH05_06435"
     CDS             complement(8785..9945)
                     /locus_tag="VFH05_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET6677885.1"
                     /translation="MTQPADRILWLLISTTLLALVVPGVALFYGGMVRRKNVINTMAL
                     PFVALAVVSLVWGLVGNGLASGTIGGEPGGPERLLSLNRGMMASVALALLAGGVVERI
                     RFSFFLIFGLCWVVAVYVPLAQWLWGGGWLTRLGSLDFAGGTVIHISAGVTALVLAIL
                     IGPRKGYGRSDMMPNHLPFSFCGAALMWVGWFGFTGASADLSMAGVTNAFVTIQLSAV
                     AATLAWMTAEWLQRDKPTALGAMSGAVAGLVAIAPGAGYVSPLSAIVIGVGAGGLCYM
                     VVNYVKIILGYDDSLDVFGMHGVGGTWGMIATGLFASTDINASGSDGLFYGYPYQFFV
                     QVVAALTGWAVAAGVTWLLYHLLTWLLAPRVDEEAESTGLDLDQHGEKGYTG"
     gene            complement(9942..10169)
                     /locus_tag="VFH05_06440"
     CDS             complement(9942..10169)
                     /locus_tag="VFH05_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677886.1"
                     /translation="MIMTQEMRPDTSACKRRLLEVLRRDGPQTLDALCELPEFTWAQV
                     LMAVDHLSRSRDISLELIGPREYQVSLVVRK"
     gene            complement(10181..>10610)
                     /locus_tag="VFH05_06445"
     CDS             complement(10181..>10610)
                     /locus_tag="VFH05_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HET6677887.1"
                     /translation="GAIAGLAMVTPGAGYVEPLSAFFMGLAAGILSYVAIMKKARFGY
                     DDSLDVVGIHGVAGIGGILLTGLLATKAVNAGGVDGLLHGNAGFFAVQGLTVIVVGLF
                     SAVGTWAILKIVDRAVGLRVPAEEERMGLDISQHNERAYS"
BASE COUNT         2218 a         3233 c         2916 g         2243 t
ORIGIN      
        1 gtaggctatg tggccttcta tcggaagaac acgagcgtca catggtggcg agcacgaggc
       61 ggaggaggcc ggagatattt tccgtttgag cggagggcat gagccgattg acctgtaagt
      121 tatagccgcc ttccacggag agttgcggtt tccagtctcg cagtggttca agctcccacg
      181 acacgattcc tttcccgccg atattgtcca ggtcgatgag tttgtcggtc gatcgcaggc
      241 cgaaatagtt tccaaccgcg gtaatgctca gccgatggct ttgcttgtag ctcatcgtga
      301 gagaagctga cggcgcatcg atccgcgcgc tagaccattc cgggcgttcc gcgcgatagc
      361 cgagcgaagg agtaatggtc agaggacgaa tcggacgcaa tgaggccgtc atggtttgtg
      421 tccggacctg gctctctgcg ccatggttca ggagatcggc ttctatcccg tagacggatg
      481 ccagcttggc atcccagatc cccccgctat agccaactgc ggcctcaagg gtgtgaggac
      541 tggctctctg cggatagaaa ttgaggggat ccatggtgtt gcttgcagta ttctgcgcat
      601 aactgaacgc gagatgcggc catgcaccct tcctccatga gacatcgatc cggttatagc
      661 tttgtttggc acgatcctgg gcagcgtcgc cttgaaggta cgtccattgc tggccgatgg
      721 cactccgtag ggccattgtc ccgatgctcc actcgctcca ggcttctctc acaccctgat
      781 cggcttggtg attgaaacct tgacctgcgt cccgaattct catgccgtag cggaccaggc
      841 ctgccgaggc cgtgacagcg aatcgcgtaa cgcgagttga gcggtccgct ccgggaagga
      901 tcgtctggtt ggccgcgact tctgtttccg tcgcaaatgc tcccttgagc caggtggttg
      961 atgccagaag cccgttgaca cccggccgct cggcgctcca aggcagattc cggacgctgg
     1021 tgggaggaac gggagatcca ttgatggtcg gtgtgacatc ggcctgaaac acaggtgtct
     1081 gcaccggctt gggaggcatt cgccattcgt cctcacttcc cgaggccggc cgtgcaataa
     1141 gtatgaccgt cgccaatgct acaactttgg catagacata catccgcgcg catagtaatc
     1201 ggcaaacgag ttctcttcaa cctttctgtg taagagcggc gtaaattttt actcaccttg
     1261 gtctgctttt ttgaggtgag gacgttttat tcttttactc tcttctcagc ctcagcttcg
     1321 gtgtgtttct gtggtgtcgt atcattgaat gcgagaagag cctccccggc gacattggta
     1381 aactttggcg taaacagaaa actgactgga ggcaagatcc accataacgt ggtctcatcg
     1441 gactgcacga tcctgacgcg ctccgcacct tcctttttcg cctcttcggt aaaatcgcgc
     1501 accgcacctt ccagtgaggc atcgccgata aagggcagag cgacagcaaa atgcagcgcg
     1561 gctttggtca tattaatgtg gactgcatct gaaccatcaa cgtcttttaa tccactaaag
     1621 tcaggcgcct tgctcgtcat tgcgcagccg gcaatggata gcagtacaat tgcactggcc
     1681 gatttcaatc cgtacatcat gagcgtgttc ccttctctgt gtttcagatt ctttccccct
     1741 tggcttcgcc atatagataa ccagcaagct atgtgccgac acacgtttaa acctaagaat
     1801 tgacttgaac agaaaaatca cgggttagaa aagatgaagg atctgctgcg tccgccgccg
     1861 acataccgaa tgaacaaaat gtcttcgtag ggacacgcgg tcctcagcgc atcccgtctg
     1921 atcagtgcag ttcgattccg actcttcata tcccctcaga gaggtggagg tgtgttgcta
     1981 gagaggttcc taggcggtcg ctgtcttttt tagaagcttg cgccgtcttg gggtggggcc
     2041 ttgagcggga agcgagctac cgagcgctct tggtgagaac ttcctcagcg gaggaaggct
     2101 ccagtggaag tgaggcgcac aaatctgccg atggacgtgg actggtactg cacgaactgg
     2161 cctcccgcat acacatctcc gcttgcgtct ccaacgggca acacgcggag taccggattg
     2221 ccgaagcctg tcgctgtggc aaaggtgaga tcgatcgtcc cgttctgatt gagccgtacg
     2281 agctggttgg cctgcatgtc attgtaggtt gtgaaagacc ctccgacgta cagatcgccg
     2341 cttgcatcgc cggccggcac gacgtgaaag actgtattgt tgaatccttt ccccgtcgat
     2401 aggaacggat tgatcacccc atttgagttc agtcgtacga ggccgttcgc cggcgtgccg
     2461 ttatagttcg tgaaggcgcc gccggcatac agacccccgt tgtccgccgg agcaatcgtt
     2521 tggacagcat tgtcaaatcc cgtccctgtc aaaaacgtcg catcaagcga tccatcttcg
     2581 ttgagccgta cgaggttatt ggctaacacc tgcttgtagc tggtaaaggc ccctcccacg
     2641 tagagatctc cacttccatc cgtcgtcggc atgatggtga aaacgggatt attgaaccca
     2701 ggccccatga taaaggtcga atccacggtc ccatcggcat tcagacgcac caggtggttc
     2761 gctgtgatcc cgttatagtt ggtgaaggct ccaccgacat agatatctcc ggttccgtcg
     2821 ccggcagaag cgatgacatg gaccgtgttg tcgaatccgg ttcctatggc aaaagtcggg
     2881 tcaaactctc cattggctga gaggcgtaca atccgagacc tctgtatccc gttgtaggtc
     2941 gtaaagtcgc ctccgacata gatcgtcgcg ccttgagtcc cagccggcac aatactgcgc
     3001 acgctgccgt taaagccgag gcctgttgca aaccccggat cgatcgtgcc atccccattg
     3061 agacgcacga tgcgaatcgc ctgtgcgccg tcgtacgtcg taaagtcgcc gcccacgtac
     3121 acatccccgc tgccatcagc cggaggagcg agcatcaaga ccgtgttatt gaatgagggc
     3181 ggctggttgg gggtgccact actgctatcg tcgccgttgt tacaggccag gacaaccagc
     3241 gccaaaacca cgacgacgcc tgcacgatag aagcgtgaca tgctgttcca aaacctcccg
     3301 tggcgccaca gaggcggctt gcagccgatg ggccgaacga tgcctgggaa gtctatgtaa
     3361 gctgtctgcc agtcgtctac ccctcaaaca gggggcaaaa acccgcctat ctcttgcact
     3421 tacccccgtt gactcggctt ctgccaaaga ctagcctttc gacataaaaa gaccggtaga
     3481 cacacaggaa tccaatttcc atcgtcatga cgccgtctga aggcgtccac cgatgacgcg
     3541 tacaggccag acacctctga gagtttttcg tctcgtgcca atcctctagt cggcataagg
     3601 cagtgatgcc aaccggtggc tcatgctgga acaaggtgtc ggatacagct cgacgtcccg
     3661 gatgcagtgt cggtactgtt cttcagaaga gtgccatcgc acgacccgcc acggatggaa
     3721 ggatttgctg tttcgcctct tcggaatgtt cccttggcgc tgcaatcggt gcggcggacg
     3781 gttttaccga cggactcgct ctggcagctg atcttccttc actcagtctt ttcatcgttt
     3841 ccccggtcag cgatccatat tcatcagact ttgagcgcaa gaatcggata gcgcccgccc
     3901 tcgacgaccg gtatcatcag tgccacaagg aggacgaata ccgatgcttc cgcagatagc
     3961 ctggaaagca gtgctcgagc ataattcccg atttgatgag gcgttcgtct atgcggtggc
     4021 ctcgacccgc gtctattgcc gcccttcctg tccatcacgg cggcctgatc gccgtcgcgt
     4081 cacatttttt gcttctccgg aacaggctga ggccgcaggc tttcgtgcct gcctgcgctg
     4141 tgccccgggt tcaccccgcg gaactcagac cgagcggctg gtcgaacagg tccgtcagta
     4201 tttggatgga cattcggatg agccggcgac tctcaccgac ctggcggcgc tcgtcggaat
     4261 gagtcccttt cggctgcaac gcgcctttac ccgagtgttc gggttctcgc ctaagacgta
     4321 tcaaaacgcc aaaagaatac aacggttcag gcaagcgtta gagcgtggag ttggaatcac
     4381 cgacgcctca taccaggccg ggtttggatc gagcagccgg gcgtgggagc aggcatcgcg
     4441 gcatatgggc atgacgccgt cggcatttcg gtccggggga aaaggcgtca tgatgcatta
     4501 tgcaagcctg cgcacaccgg tcggcttctt gctgatcgct gccactgagc gcggtctctc
     4561 gtcggtcatg ctgggaggaa gcgagccgtt gttggtagcg aaactgaaac gacagtatcc
     4621 gaatgcgcaa ttgcgccgaa atcccaagcg attgcttccc taccggcaag ccctggctcg
     4681 gtgcctgaac ggaaaccctg caggagccgg attgaccctc gatgtgaaac ccaccgcctt
     4741 tcaattccaa acatggatgg cgctccagca gatccctgcc ggatctacca gaacgtatcg
     4801 ggagattgcc tgcacaatcg gccgaccggc ggcttcgcgt gccgtggccc gtgcctgcgc
     4861 aaccaatcct ctcgccctgc tgattccctg ccatcgggcc gtcggccaag acggccgcct
     4921 tgcgggttat cgatggggca tcagacgaaa gcagcaatta ttgaggctgg aggcagcccg
     4981 ccgtcagcac ggccgccctt caaaaaggtc gtcctaatcc atgaaggcgc gaaggaggtc
     5041 ggcaagcgac agcacaccga tgatggtgcc gtcagccgtg acaggcaaat ggcgtatgcc
     5101 ctgcttcttc atgaggttca tcgcttctga caacaattcg tcttcttcta tcgttacaag
     5161 cgatttgctc atgcaggtcg tcacggtggt agtgttcgga tcaagccctt tggccacggc
     5221 tttcctgctg agatcggaat ccgtgacgat gccgatgtag cgcgacccgt cgtccacgat
     5281 cagcgatcct accttgtatt tctgaagcaa cagaccggct tccttgatcg tggcggcacg
     5341 gtggatactg cgcacgtcgt gggacatgta gtcttccact ttcgcctgca acattccctt
     5401 tccgtttgac tgtttggctg actgtatcag gcgacgcatt tccagatcgg ccatgcagct
     5461 ttccagcact tgccggcgct gctgcaggct ttcacgctcg atattgtgcg ttccgttctc
     5521 ctgcgcttcc tccggcaaca gagccgactc gccgttcttg gcatagaaat aggcctcggc
     5581 ttcatccgac gcagctccga acacctggct gatcagctct tcagccgact catcgaagtc
     5641 ctcgatggtc gtcgtacttc gcttgcggga gagaaacggc cgtagcttga tcagttgttg
     5701 tttgaaccgc tccaggtagc gatgaagaat gtcggtacgc gttaatcccg tgcgaactcg
     5761 tttcggcatt gcatccctcc catggcggga cgagaatagc gcaacgcaga cgtttgctca
     5821 aggggagcag ccatcggaca acctccccat ccaatccgca gcctacccat ccactcctcc
     5881 cctcttcccc tgcatctcct tgttatagaa ttccagcatc tgagcgattt cttccggggt
     5941 catgcctttt tcgatccacc ggtggctttc gttccgaagc agttcttcgg ccacctgggg
     6001 cgacaactcc cgaacgagcg gcaggaactt ggtgtagaaa ctcttggcca cctcgtaaaa
     6061 tccctgccaa tgcacatagt ccggcgccac tttcgatagc ccgtgccggg cccgacgccc
     6121 ttcgtggtgc cacagttcgt agaacaccca ctcgatcttt tcgtcgtatg gagtcggcgt
     6181 gagctttttc atggccaaga gcttgtccat ggcttctttg gccggcttgc cgaatttgtc
     6241 gttgtacaag ttgatgccct ggtccatgga cgtaaagaac cggtccacga tctgctcgct
     6301 gtgacaggcg gagcagattt gcttcatgga tttccgccgg tcctcatagt tttcgagacg
     6361 cgtcgaaatg accggccgca gcgtccaact gatgcggtct cccacatcat gcgtcacctc
     6421 ttgcgtcccg gtcgcgctca tatgacaggt ggcacaggtc ggatagagat aatctttgcc
     6481 gggatgccac ttctccgtgg ggtccgcgag cttcatcttc tctttgttgg cctcgtacat
     6541 gacgccgtgc ttgctctcat aataggcttc gatctgcgga tgatccggcc ccatgtggca
     6601 gcggccgcag ctttcgggct gccgcgcttg agcgatggag aagctgtgcc gcgcgtgaca
     6661 agcggagcag gtgcccttgg agccatcggg gttgacccga ccgattccgg agttgggcca
     6721 cgtcgccgga tgaagcttgc ccttttccat gaccttcacg acgctgccgt ggcagccggc
     6781 gcacccagtc gtcacgacct caggcccttc gacgacgttg ccgagaaaat tgtcgagcga
     6841 cccggtgaat tgggccgcct tggcgtgatg ggacttggcg aactctttcg cttcccggtc
     6901 gtggcaccgc atgcaatcct tcggcgtgac cagcgtcgag atgagaaacc cctcgtgatc
     6961 gtacccgtcc gcttcgcctt tttcggcccg atgacattcc acacaaccga tgcctttcgg
     7021 tgcatggcgg ctgactttcc aatcgttgat gccgccgacc gccacgtcct tggccatgtg
     7081 gcaatcgatg catttcttgt tttccgccga cacgaaggga cggaggcctc ccccccgtcg
     7141 ctcttcgacc tggacgtacc cgacccccgt cagggcgaga aacagaatca ggcagagtcc
     7201 cccgatcatg tagcgggcga agttttgctt gccgttgctc gcagccatat ggcgatcgtc
     7261 atggccgctc tcttccagct cgtccatcgg ttcctcctga ttcacatctg cgtatcgagg
     7321 tacatcaaaa agaccatggc gccgacgact gcagcggccg caatagcgtt cacgacgtcc
     7381 atcggccatc cgcgctcagc cagcatgcgg tcaagaaacg gataaatgac aaagagtccg
     7441 gcaaatccca gcaagctcca cagggccacc gggccaggca gcaacgtgat ccacatgaac
     7501 ggcgccgaga aataccaggg cggcgaaacg tcggacgcga cttcctgggg attcgccggc
     7561 ggcccgagca gcggcgggaa gatcatcgca atgttcacga tcaggaggag cagtccaacg
     7621 gccacgactc ccatcttgag cgtgtgctcc ggatagaagg gatggaagcc gcggcttccg
     7681 cgcacgtcgg cgaatcccag caggcgcacg atgagtacat ggccgaggac caggcccccc
     7741 agcaacaccg gcagtaactt cgtatggagg tcatacaacc tcagcaaggt gacacccgac
     7801 acgtcttcac ccccgcgcag caaatgcagc agtggtgtgc cgatcacggg aagcgtaccg
     7861 accatattcg tcacgaccgt catgccccaa tacgagacgt tgtcgaagac gagcgagtag
     7921 ccggtaaaac ccatggccaa ggtgacgaat aggacgatcg atccgcttac ccatttcaac
     7981 tcgccgggtg ttcgataggc gcgggtaatg aagactcgga tcacgtggaa gaggagaaac
     8041 aagatcatga gatcgacgga cgttttgtgc agaccccgga cgaaccagcc cagatagatc
     8101 tcgttggtaa tttgatcgac actctcatag gctttttctg gagaaggcac gtaatagaac
     8161 gtcaacagca ggcccgtcgc gaccaagatg ccgaaaagcg taagcgggat ggcgccgagt
     8221 gtgtacggcc accagtcgag atgctgcggc agatccttct ggatgtattc gatccacgag
     8281 gtcttgcccg tgcccttcgg atcaatgtgc tgtgtggtcg tggagtccat ttcgcttatg
     8341 cgcttgcgat attgacgtac aactgctcgc cccgtttgac gatctgaaac cggtcgagcg
     8401 gccgtggggg tggaccggca atgttgacgc cattcttgtc gaaggtgcct tgatggcagg
     8461 gacagacgaa ttgttcacgt tctttgttcc acgacaccag gcaccccaga tccgtacagc
     8521 gccgggaaaa cgccgccacg ccatcctgcg tcttgagcac gagtatcttg tggcctccga
     8581 gatccatggg caccgcatca ccgagcggaa tgcgtgtctc agcaccgatc aacaccgtct
     8641 ccggctgctt gtcggtcctg gtgggcagca gaaactgcat aaaacggaac ccgagcgtgc
     8701 cgaggccgaa cagcatcccg aaccccatga cggcctgact gagaaagcgc cggcggccgg
     8761 catcgtcgtg tacaatggcc atatctaccc cgtgtaacct ttctccccat gctgatcgag
     8821 gtccagtccc gtcgactccg cttcctcgtc gacacgcgga gccagaagcc acgtgagaag
     8881 atgatagagg agccacgtca ctcccgcggc gacggcccat ccggtcagcg cagccaccac
     8941 ttggacaaag aattgatacg ggtatccata gaacaatcca tcgctgccgc tcgcattgat
     9001 gtcagtggac gcaaacagtc cggtggcgat catgccccag gtgcctccga ccccgtgcat
     9061 cccgaagaca tccagtgaat cgtcgtaccc cagaatgatc ttgacgtaat tgaccaccat
     9121 gtagcagagg cctccggctc cgacgccgat gacgatcgct gacaacggac tcacatagcc
     9181 cgcccccgga gcgattgcga ccaaccccgc gacggccccg ctcatggccc ccagggcggt
     9241 cggcttgtcg cgttgcagcc attccgccgt catccatgcg agcgtcgcag caaccgccga
     9301 caattgaatg gtcacgaagg cattcgtcac gcccgccatc gaaagatctg cggaggcgcc
     9361 ggtgaatccg aaccagccca cccacatgag cgccgccccg cagaaggaaa acggcagatg
     9421 gttcggcatc atgtcggatc gtccataccc tttccgcggt ccgatcagga ttgccaacac
     9481 cagcgcggtc acgccggcgc tgatgtgaat cacggtgccg ccggcgaaat ccaggcttcc
     9541 aagcctcgtc aaccacccgc caccccacaa ccattgcgcc aacggcacgt atactgccac
     9601 gacccagcac aatccgaaga taagaaagaa tgaaaagcga atacgttcaa cgacgcctcc
     9661 tgccagcaaa gccagcgcca ccgacgccat catcccccga ttcagcgaga gcaatctctc
     9721 tggacctcct ggttcgcccc cgattgttcc cgacgccaga ccatttccga cgaggcccca
     9781 aacgagcgac accaccgcca gtgcgacgaa tggcaaggcc atcgtgttga taacgttttt
     9841 cctcctcacc atgccgccgt aaaagagcgc gactcctggg acgaccagcg ccaacagcgt
     9901 cgtcgagatc aacagccaga ggatccgatc ggctggttgc gtcatttgcg aactaccagc
     9961 gagacttgat attcacgggg cccgatgagc tcaagcgaga tgtcgcggga ccggctcaaa
    10021 tgatccacgg ccatcagaac ctgcgcccag gtgaattccg gcagctcgca caaggcatcg
    10081 agcgtctgcg gtccatcccg gcgcagcacc tccagcagcc gccgcttgca ggccgacgtg
    10141 tcaggtcgca tttcttgggt catgatcatg ccttcctcct ttatgagtac gcccgctcgt
    10201 tgtgttgact gatatccaat cccattcgct cttcttccgc aggaacacgt aaacccactg
    10261 cccgatccac gattttcaga atggcccagg tgccgaccgc cgagaaaagt ccgaccacaa
    10321 tgacggtcaa cccctgcacg gcaaaaaacc cggcattgcc gtgcaacagt ccatcgacac
    10381 cacccgcatt gacggccttc gtcgcaagaa ggccagtcag gagaatgccg cctatcccgg
    10441 ccaccccgtg gatgccgacc acgtccaacg aatcgtcata gccgaaccgt gccttcttca
    10501 tgatggcgac ataggacagg attccggcgg ccaaccccat aaagaacgcg gagagcggct
    10561 ccacatatcc cgctcccggg gtgaccatgg ccaaaccggc aatcgcgccg
//
LOCUS       DASSCQ010000091         6282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1476721, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000091 DASSCQ010000000
VERSION     DASSCQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476721"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..546
                     /locus_tag="VFH05_06450"
     CDS             136..546
                     /locus_tag="VFH05_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6677888.1"
                     /translation="MINVLLVDDYPIMQQLLRDILQRYPDICVVGEAQNGEEAVLQSM
                     KLKPTVVIIDINLPTISGMEATRLIKLERPSTVVVGLTAGVPDQTVTAMRGAGAAAVL
                     NKGDLLNALYPTIIEAMRSSAKPAFASASASSKH"
     gene            1009..2538
                     /locus_tag="VFH05_06455"
     CDS             1009..2538
                     /locus_tag="VFH05_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6677889.1"
                     /translation="MPFTSSGKETFAKRLQGRKYLVGSLVLSALAIRMLLAQRKQAEA
                     ELKAIEWLLREGGEPFHMPKPQPYGDLTELNTELTVLRRVGKDLLINVAGDALHLIGT
                     SGEVCEKNGDYASCLVASDWCRFLDQASREQCGTVDNRAALASGRWHCRESGREASTL
                     AMETGKPVDIPCKGGINLYAAPIMVDGETVGSITIGYGDPPRDHKQLTEVAERYGVQV
                     ERLRELSANMPSRPQFIVAVAKARLRTEAVLLGEIIKRKGAEEALADLTKNLEVRIAK
                     RTQQLRALATELNLAEQQERKRLAVELHDHLQHLLVLGKLRIGHGKRYAPPIPVLTEA
                     LTQVDQVLSDALRYTRTLVAELSPAVLRDHGLLAALKWLSEYMQKLSVTVAVNMPEEL
                     HVILPEDQAVLLFQSVRELLINASKYAGTSAATVTVECRDDQLRIQVRDDGAGFDVAA
                     VTAASSASTGGQSYKFGLCSILERMQALGGSFDILSAPGRGVTATLTLPLSQSETRPA
                     A"
     gene            3649..4137
                     /locus_tag="VFH05_06460"
     CDS             3649..4137
                     /locus_tag="VFH05_06460"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET6677890.1"
                     /translation="MSSQNRNIGGFTLIELMVTVAILTVAVGLAVPEFLRWHVQSQLR
                     QATTEIATQLMLARMAAMNRNRSVDVTVQSSGGAVHISAVAPASGAAVIKDTSFPTRV
                     TAVVGSPVVVSFSSLGMRTTGGTGTQTIGVCDTYRRQYSVSIIPSGKVNWSNNPSGTA
                     CP"
     gene            4157..4651
                     /locus_tag="VFH05_06465"
     CDS             4157..4651
                     /locus_tag="VFH05_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677891.1"
                     /translation="MQLNRVHNEQGFSLLEGMLAAVLLGTGLLAMSAMQGIAFVKNVD
                     SNELTRITTLASDMMERIQFNRRSAISYNGIDTQSPTNCNAISAAAQPQARGDCLLWD
                     SLVDGTQLQDVRGRVTVSDIIDPIVLAQRNVTVTITWKGSLKSDSTVKRDRTLTINRV
                     VAPE"
     gene            4668..5726
                     /locus_tag="VFH05_06470"
     CDS             4668..5726
                     /locus_tag="VFH05_06470"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HET6677892.1"
                     /translation="MRRSIAQAGFTLIELMVGMAVSVVVVAAAFTILTTTSKSLRANE
                     QIVDTQQNLRMAMEMLSRDFKLAGFGNPGVPIGNCTSGIVPADQNTAGVDTGPDSVQL
                     LVPTTKASGANRWTLSLPTNAAGTTVITLQPGAVADMANSGLTANASYISIGGAATAQ
                     VTGFNPTASTINVSIPPPLWFQTEDRVYLLQCIRYRVVPAPDPANLCGGVAPCLTRGV
                     AGVVAGANAEAPIAEGIEDLQLAYACDGCVTGINSGIPDHIIDDQPPASNSFDQADFL
                     SNVSWTAAPLTPDKIQLVQVALVARQSQNDQGFGESNSAMVGASSLTVTGDHSLPADS
                     NHRRRILIKTVETRNVGM"
     gene            5731..>6282
                     /locus_tag="VFH05_06475"
     CDS             5731..>6282
                     /locus_tag="VFH05_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677893.1"
                     /translation="MAQSKYPRRGALVRLNDPRGMAMLTVMMVLLMLTVLGIAAITAS
                     GLGNNMAGLQRTMEASANAAESCLGTGVNIIQHVLLAENGRQIPSALLSSTTGGPVPS
                     GNKTVLESEIAGNSQNNTDVPIGSGSIVAAPNLQMTVGPYAVVGDIDFLYVKQRAGSG
                     QMQFSGYEGAGAGGGNDVDAFYRL"
BASE COUNT         1416 a         1724 c         1771 g         1371 t
ORIGIN      
        1 tgctgaaaat acaacgcctc gccgtcgcta gtggcctccc tccgaccgaa tctctcaccc
       61 ctcaaccatt taacccttcg gaggggttga gcttctatcg ttcacacgcc tatgcatccc
      121 atctcagagg ctcgcatgat caatgttctg ctggttgatg actatcccat tatgcagcaa
      181 ctgctgcgtg acattttaca gcggtacccc gatatctgcg tggtcggtga agcacaaaat
      241 ggtgaagagg ccgtcctcca aagcatgaaa ctcaaaccga cggtcgtcat catcgacatc
      301 aatttgccga cgataagcgg catggaggcg acgcggttga tcaagcttga aagaccatcc
      361 actgtcgtcg tcgggctgac ggccggcgta ccagaccaaa cggtgactgc catgcggggt
      421 gccggagcag cggccgtgct gaacaaagga gatttgttga atgcgctgta tcccaccatt
      481 atagaagcca tgcgaagctc agcgaagccc gcgtttgcat ctgcctctgc cagttccaag
      541 cactgatcca tcacaccggt tgttgcaccg ccgtccatgt gaatccttgg ccttcatgtg
      601 cctgtccagc acatctcacg cttgccatcg attaaccctt cggcaagacc gaaatgttcc
      661 gctctcgcgc aactcccaga tcgtgaaaaa tccgtattcg cggtacgtct ccagcggttg
      721 aagaaccaaa tcacctgtag gacggatcct ctagcactaa tcccagtagg agatagtgcc
      781 tctgttctat aattttgaac ccttcgccat gaagacctcg gaaaccatct gatcctcagg
      841 agtggaggtg tgcttgccga cgaaaccgag tcggtccgga acagtgctcc ttcggagttc
      901 ttcagcacgc cgttccgctt tggcaccttc ctgtctatag gcgtgcgccc ggggtagcag
      961 cagtggattt cgatgtttgc cgtacgttga ggtgttcaga tgcgactcat gccgttcacg
     1021 tctagcggca aggaaacctt tgcgaaacgt ctgcaaggcc ggaaatatct ggtgggttct
     1081 ctggtgctct cggcgcttgc catccgcatg ctactcgccc agcggaaaca ggcagaagca
     1141 gagctcaaag ctattgagtg gctgctgcgt gaagggggag agccgtttca catgccaaag
     1201 ccgcagccgt atggggatct gactgagctc aacactgaac tgaccgtact tcgcagggtc
     1261 ggcaaagacc ttctcatcaa tgtggccggt gatgcgctcc acctcatcgg tacctcgggt
     1321 gaggtgtgcg aaaagaacgg agactacgcc tcctgtcttg tggcatccga ctggtgtcgc
     1381 ttcctggacc aggcgtcgcg cgaacagtgc gggacggtag acaatcgggc tgccttggcc
     1441 agcggccggt ggcactgtcg cgaatcaggg cgagaggcgt cgaccctcgc catggagaca
     1501 gggaagccgg tcgacatacc ctgcaaaggt ggaatcaacc tgtatgcagc accaatcatg
     1561 gtcgatggag aaacggtcgg aagcatcacc attggctatg gggacccgcc cagagaccat
     1621 aagcagctga cggaagtggc cgagcgctat ggtgtccaag tggagagact ccgagaactg
     1681 agtgccaaca tgccctcccg gccgcaattc atcgtggcgg tagcgaaagc tcgattgcgc
     1741 actgaagcgg ttttgctggg ggagatcatc aaacggaaag gcgctgagga agccttagct
     1801 gatttgacga aaaacctgga agtgcgcatt gcgaagcgaa ctcagcaact ccgtgccctg
     1861 gcgactgaat tgaatcttgc cgagcaacag gagcgtaagc ggctggctgt cgaactccac
     1921 gatcacctgc aacatctcct cgtactggga aaactgagga ttggacatgg gaagcggtat
     1981 gccccgccga ttccagtttt aaccgaggcg ttgacacaag tcgaccaagt gttgtccgac
     2041 gcccttcgtt acaccaggac cttagtcgcc gaattgagtc ccgccgtact gagggatcat
     2101 ggactgctcg cagcactcaa atggctgagc gagtatatgc aaaagctctc tgtgaccgtc
     2161 gccgtgaata tgcctgaaga actccacgtc atcctgccag aggatcaagc cgtgctgctc
     2221 tttcaatctg tgcgtgaact tctgatcaac gcctcgaaat atgccgggac cagcgcggcc
     2281 acagtcacag tcgaatgccg cgacgatcag ttacggatcc aggtacgtga tgacggcgcc
     2341 ggcttcgacg tggctgccgt gactgctgct tcgtcggcct cgaccggtgg acagtcatac
     2401 aaattcggcc tgtgtagtat cctggagcgg atgcaggctt tgggaggatc gtttgatatt
     2461 ctatctgccc ccgggagggg cgtcaccgcg acgctgaccc tcccgctgag ccaaagcgag
     2521 acgagacctg ccgcataact ggcctggcga gaaagcttgc tggtgtgaaa cattctgagc
     2581 gaccgtggtt ttcgtggagt tagtttcatc cgacctgttc ccctcacgct gcgtctcgac
     2641 ttcactgtta accagcacat gacacctgag aatgctcggc cctgagacga gttcctaatg
     2701 tctcgttctg ctgaacaaag ccgtctaact tttcagccgc tctcaaacag tccgacatgc
     2761 gccgttgtcc cctgagtcat caatcgctat cagatgctca atctccccat cacacgagat
     2821 cagacctttg acgattttcg atcctttgcc acaatttgct aaaactgctc gggcacagag
     2881 gctcgcagat ggttgattta tctcatttct tgacccggca taagtcctgc tgcgctctaa
     2941 agcagaaggc gctactggat tgggtagccg gagatcactg atctgtcggc acgatgtcga
     3001 gtggctgcaa aaaaaggagg catcatatga tcgcacgtcg gttggctctt gcaatcgcag
     3061 gtgcatttgc ggtttctgcc ttgatggcgg ggcctgcaca agcggtctgc gacccgcatt
     3121 gtgctccgaa ggcgaataaa agcggcgaag tgcgaggcct gaaccgcgcg aatactgtcg
     3181 ccggtgacca tgggcagcac gggcgggaca atgccgttgc caaacaggat ctacacaagc
     3241 cgggtggctc tggtgtggtc cagacaccgg caccggtacc tcctgcgtcc gtgcctcctc
     3301 cgcctgtagt tcctgccccc gaaccttgcg tagggtgtta ggcaaagcgt cttgctacgc
     3361 ttggaggagg cgccgcgatc gaggcgcctc ctccccaaca ctcatctaaa cggcattgca
     3421 gatcccaacc ggtgccaact cacagaaatg atcatcacaa gctgtttttg tgagtgtaca
     3481 agccgaaaat tgtcagccga cgagcaaaaa agaaaaactt cctgccccta gggttaattt
     3541 ctcccacgaa actaatgagt tacacactta accaaacggt taatgaaggc atgtcctttg
     3601 ctctcaccct cagggccaag tatggccttc accggaggat gaccctctgt gtcttctcag
     3661 aatcgaaata tcgggggctt cacgttgatc gaactcatgg tcacggtcgc cattttgacc
     3721 gtcgccgtag gcctagcggt tccagagttc cttcggtggc atgtgcaatc gcagcttcga
     3781 caggccacga cggaaattgc cactcagctc atgttggcgc gtatggcggc aatgaaccga
     3841 aatagaagtg tggacgtaac cgttcaaagt tccggtggag cggtgcatat ctctgccgtt
     3901 gctcctgctt ctggggcggc cgtcatcaag gatacaagtt ttccaacccg tgtcaccgct
     3961 gtagtgggaa gtccggtcgt ggtgagtttt tcctcgttgg gcatgcggac aacgggagga
     4021 acaggaactc agaccatagg cgtgtgtgac acgtatcgac gtcaatactc cgtgtcgatc
     4081 attccgtcgg gaaaagtgaa ttggtctaac aacccgagtg gcaccgcttg cccgtgatga
     4141 aagattgtga gacgtcatgc aactgaaccg agtccacaac gaacaggggt tttcgttgct
     4201 ggaaggaatg ctggccgcgg tgcttttggg cacgggtttg ctggcgatgt cggcgatgca
     4261 gggtatcgcg ttcgtcaaaa acgttgattc gaacgaactc acacgcatta cgaccctcgc
     4321 gtccgacatg atggaacgta ttcagttcaa ccgacggagc gccattagtt ataacgggat
     4381 tgatactcaa agtcccacca actgtaacgc cattagcgcc gcggcacagc cgcaggctcg
     4441 aggagattgt ctgctgtggg acagcctcgt agacggaacc cagcttcaag atgtcagggg
     4501 cagagttacc gtatccgaca tcatcgaccc gattgtcttg gcccagcgca atgtgacggt
     4561 aaccattaca tggaaagggt cgctcaaaag tgattccacc gtgaagcgag atcgaacatt
     4621 gaccatcaac cgagtggttg caccagaatg ataggaaaga tttgacgatg cgtcgatcaa
     4681 ttgcacaggc tggattcact ctgattgaac tcatggtggg tatggcggtg tccgttgtgg
     4741 ttgtcgcagc ggcattcaca attctcacaa ccacgagcaa gtcattgcga gccaacgagc
     4801 aaatcgtcga tacgcagcag aatctccgga tggccatgga aatgctgtct cgagatttca
     4861 agctggctgg ctttggaaac cccggagttc ccattgggaa ctgcacgagc ggaattgtgc
     4921 cggccgatca gaataccgct ggcgtcgaca ccggaccgga ctcggttcaa ttattggttc
     4981 cgaccacgaa ggcatcaggt gcgaaccgct ggacgttgag cctgccaaca aatgcggcgg
     5041 gaaccacggt gatcacgttg cagccgggag cggtcgctga catggcgaac tccggcctca
     5101 cggcgaacgc gtcgtatatt tctattggcg gggcagccac ggcgcaagtg acagggttta
     5161 atccgactgc cagcaccatc aatgtgtcga ttcctccgcc actctggttt cagacagaag
     5221 accgggtcta tctgctgcag tgcattcgtt atcgcgtcgt gcctgcccct gatccagcca
     5281 atctctgtgg cggagtcgcg ccctgtctga ccagaggagt cgcaggggtt gtggctggtg
     5341 ccaatgcgga ggcgcccatt gcggaaggga ttgaagatct tcagctggcc tatgcctgcg
     5401 atggctgcgt gacaggcatt aacagcggaa ttcctgatca tatcatcgac gatcagcctc
     5461 cagcctcgaa cagctttgat caggcggatt ttctcagcaa tgtctcgtgg accgccgcgc
     5521 cgctcactcc ggacaaaatc caattggtac aagtcgcgtt ggtggccagg cagtcgcaaa
     5581 atgatcaagg cttcggagaa tcgaacagcg caatggtcgg agcatcttcg ctcaccgtaa
     5641 cgggtgatca ttcgttgcct gctgattcca atcatcggcg ccggattttg attaagacgg
     5701 tcgaaactcg caatgtgggt atgtagcgtg atggctcagt cgaaatatcc tcgtcgaggc
     5761 gctctcgttc gtctcaacga cccacgaggc atggccatgt tgacggtcat gatggtgctc
     5821 ctgatgttga cggttcttgg cattgccgcc attacggcca gcgggctcgg aaacaacatg
     5881 gctggattac agcgaacgat ggaagcctcg gcgaacgccg cagagtcgtg tctaggaacc
     5941 ggcgtcaata tcatccagca tgtattactg gcggaaaacg ggcgtcagat tccatctgct
     6001 ctgctgagca gcacgactgg aggaccggtt cccagtggaa ataagacagt gttggaaagc
     6061 gaaattgcgg ggaattcgca gaacaatacg gacgtgccga ttggatccgg ctcgattgtg
     6121 gcggctccca acctccaaat gaccgtgggc ccttatgcag tggtcggcga tatcgacttc
     6181 ctgtacgtga agcagagggc cggaagcgga caaatgcaat tctctggtta cgagggagcc
     6241 ggcgcgggcg gtggaaatga cgtggatgcc ttttacagac tc
//
LOCUS       DASSCQ010000092        10149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_616782, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000092 DASSCQ010000000
VERSION     DASSCQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616782"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..142
                     /locus_tag="VFH05_06480"
     tRNA            68..142
                     /locus_tag="VFH05_06480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Val,seq:gac)
     gene            302..2236
                     /gene="thrS"
                     /locus_tag="VFH05_06485"
     CDS             302..2236
                     /gene="thrS"
                     /locus_tag="VFH05_06485"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET6677894.1"
                     /translation="MPKVTLKNGKSFDVPPGYTIAQALAAGGIPVGPEVLAASVNGRP
                     TDLSAPLTADSTIDPLGFDTAEGREIYRHSSTHIMAQAVKELFPSAQLTIGPALEDSF
                     YYDFAYDRPFTPEDLENIEQRAREIAKRKLAVTRRDLTKQEALELFRSRGEHYKVELI
                     EGFPDQEPISIYTQGEFVDLCRGPHVPSTGHVGALKLLTTAGAYWRGDERNPMLQRIY
                     GTSFPTKAELDAHLAKLEEIKRRDHRKVGKELDLISVQDEIGPGLVLWHPKGAVIRLL
                     IENFWREQHLRDGYELVYSPHVARLDLWKTSGHVDYYRDNMFAPMKLEGSEYQLKPMN
                     CPYHIMIYKSHLRSYRELPIRYGELGTVYRYERTGVLHGLLRVRGFTQDDAHLFCRPD
                     QIEAEVSRVLDFTFFILRTFGFMEFEVFLSTRPNESVGAEEHWTLATSALEAALKGRN
                     ISFHLDPGGGAFYGPKIDIKIKDALGRSWQCSTIQIDFNNPERFELSYIGEDGKSHQP
                     IMIHRALMGSIERFFGILIEHYAGAFPTWLAPVQAVVMAITDNQREFVTEVTAHMKAS
                     GFRVESDIRNEKIGFKIREAEKAKIPFMFVVGDREVQSRSLSVRGRSGANLGTMTVQG
                     ALDLLKTEVERAGQQLSPTS"
     gene            2265..2756
                     /gene="infC"
                     /locus_tag="VFH05_06490"
     CDS             2265..2756
                     /gene="infC"
                     /locus_tag="VFH05_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET6677895.1"
                     /translation="MNREIRVREVRVIGPEGEQLGILPTPEAFKQAQEGGYDLVEVAP
                     TSVPPVCRIMDYGKYKYELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIKIRQIKG
                     FLEEGNKTKVVLTYRGREMANQEMGRVVMNSVISELSEAGTIEYAPRMEGRSLIMIVA
                     PKN"
     gene            2789..2983
                     /gene="rpmI"
                     /locus_tag="VFH05_06495"
     CDS             2789..2983
                     /gene="rpmI"
                     /locus_tag="VFH05_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET6677896.1"
                     /translation="MKMKSHSGASKRFHRTGTGKFMRKRAGKRHILTSKNRERKRRLS
                     GSVVVDETKAAALKRLLPYT"
     gene            3019..3387
                     /gene="rplT"
                     /locus_tag="VFH05_06500"
     CDS             3019..3387
                     /gene="rplT"
                     /locus_tag="VFH05_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET6677897.1"
                     /translation="MPRAKGGPKTRARRKKRLKLAKGQYGAKSRLFRSATESVDKGQQ
                     YAYTGRKNRKRDFRQLWIARISAATRAHGLTYGRFINALKKANVLLDRKVLSDMAIKD
                     VAGFEQLVGLAKQHLTPATA"
     gene            complement(3440..5161)
                     /gene="pheT"
                     /locus_tag="VFH05_06505"
     CDS             complement(3440..5161)
                     /gene="pheT"
                     /locus_tag="VFH05_06505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET6677898.1"
                     /translation="MPTISIYKSDLESLLASDGRAKRPVTIPQLEEWLMLVKGELKEH
                     VAETGELRIELQDSNRPDLWCCEGIARQIRIRQQRKLRAYPFFSATSKKAFRRLNVAP
                     GLERVRPYVAACASTGYRVTEEGLAQLIQTQEKLSEIFGHKRKTVSIGLYRLARIQFP
                     VTYELVKPDAAKFTPLGMETTMTLGEILMVHPKGLEYGHILADHDQLPILRDAIGQPL
                     SFPPIINSREIGEVQVGDDALFVEVTGTDLPMVVLTLNIFAANLADRGAVIDPIDVRY
                     PMKTPFGKQVVTPRDLDITRTIPIQTIEQALGQTIGAQAVKQALEVYGYVVTPSKGTV
                     KAKLPPYRQDLMHPMDVVEDVAISRGYGDFSPVMPAQFTVGGLSKIEQTSDRARELMV
                     GMGFQEIISNILGSPEQYRDAMRLNETLWGEMVEVENVMSLSFSCLRQWMLPSLLRVE
                     AASGRAFYPHRLFEAGDVAIPDRRHELGSRTETVVGAAIAHAAAHFSEIHSCLDILFY
                     HMGKDYTLEPVQHPSFLEGRAGQIVVSGKPLGIIGEVHPEVLERWQIAVPVAAFDVNL
                     SRLVEVH"
     gene            complement(5165..6733)
                     /locus_tag="VFH05_06510"
     CDS             complement(5165..6733)
                     /locus_tag="VFH05_06510"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET6677899.1"
                     /translation="MDISSLIDSLHPLEIKVLTAFGTNPSGLVLDTDQLAKATNLEAS
                     QLSMAIEWLIAKSLLAVDKETITPVVSLTPVGERYHGKEAPIEEVLAAARSAAGTGGR
                     LTIRDLQSQEGLDPSDVSKAVGRLKKEGALLIVQGGCIESTGRPSPTADRVRTLLQQV
                     HEGPRELESFAEPDRQIIQEYAVRRGNAKEPFRVDDRVARSFRVAPAGLAAAEHLVQH
                     DVAAEEVSQLSPEMLKDGSWRAKRFRKYTISLRAPRISTGKRHPYREFLDTVKSKLVS
                     MGFREMRGALVETEFWNMDALFMPQFHPARDIHDVYFVKHPTCATHVAEPFLSRVAQA
                     HRDGGNTGSMGWRYAFDVERAKRLVLRSQGTAVSARTLASGPATPAKYFSIARCFRYD
                     QVDATHATDFFQVEGIVLGDDINFRTLLGLLNLFAREVAQAKDVKFLPAYFPFTEPSV
                     ELHVRHPRLGWMELGGAGLFRPEVTLPLGVKVPVIAWGLGLDRMAMVALGIHDIRELF
                     TNNLDMIRTTRGVF"
     gene            complement(6742..7425)
                     /gene="rpe"
                     /locus_tag="VFH05_06515"
     CDS             complement(6742..7425)
                     /gene="rpe"
                     /locus_tag="VFH05_06515"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET6677900.1"
                     /translation="MTARTALIAPSILSADFARLADEVAAVERGGADLLHVDVMDGHF
                     VPNLTVGPPIVESLKKVTKLPLDVHLMITNADAFLADFASAGADYLTVHVEACPHLHR
                     TVQAVKERGVKAGVTLNPATSLHTIEEILPDIDLLLIMSVNPGFGGQTFIESCLHKVS
                     TARRMLDGIGSRALLEVDGGVKVDNAARILAAGADVLVAGSAIFCGDDYAVTIAALRA
                     ARQPASVHR"
     gene            complement(7422..8813)
                     /gene="rsmB"
                     /locus_tag="VFH05_06520"
     CDS             complement(7422..8813)
                     /gene="rsmB"
                     /locus_tag="VFH05_06520"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET6677901.1"
                     /translation="MPIHSARSVALSVLVESAHSTEGLDTLFDRALLRTPLDGRDRAL
                     ALEITYGVCRRLLTIDWRLEPVLDKPLARLPIAVQMLLRMGAYQVLFLDRIPASAAVN
                     ETVNLAKAQVKKLHRDWGGFVNAVLRALIREPIVQWPSVHDDAAYALAVRYSVPEWLS
                     RRWLDRLGLAVAQTVCEQASLIPPLTLRVNLLRITREELLRRMEKAGIQGKPTRISPA
                     GITLVEGGSVAMLPGFAEGHFYVEDEAAQLIPFLLDVRPGNLVLDACAAPGGKATHIA
                     ELMEDQGRIYAVDRSAGRLRAVQINSSRLQLKSIVPIVGDLRDSSWEDAAAKMVKTPG
                     RLWFDRILVDAPCSGLGVLRRHPESKWRKGQNAFARHQAVQSHILASAARCLRPGGVL
                     VYSTCSTEPEENEVVIDGFLRSHAEFRRMSVAPWLPEPGRQFLTARGDFSTMGNVDSM
                     DGFYAARLTRMDS"
     gene            complement(8946..9887)
                     /gene="fmt"
                     /locus_tag="VFH05_06525"
     CDS             complement(8946..9887)
                     /gene="fmt"
                     /locus_tag="VFH05_06525"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET6677902.1"
                     /translation="MRIVFMGTPEFAVPSLEALLKGPDQVVGIVTQPDRPKGRGQVLT
                     PSPVKLIAQREHIPLLQPTKMKDGDFITTLSSWKPDYIAVAAFGRILPPAILSLPPFG
                     CINVHGSLLPKYRGAGPIQWALINGETETGITTMLMDEGMDTGAVLLQEAMTINSEDT
                     AGSLSVRLAELGGQLLVKTLAQLRAGTLIPRAQDHSQATLAPLLKKEDGIIDWTMPAT
                     AIANRIRGLDPWPGAYTFAKTERWTIYRAVVLDEPARGVPGEVTAVQKDALHIATGRG
                     VLAIYEVQAAGGRRMPVAQYLTGHPIGAGMRLGAHTQ"
     gene            9971..10047
                     /locus_tag="VFH05_06530"
     tRNA            9971..10047
                     /locus_tag="VFH05_06530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10005..10007,aa:Met,seq:cat)
BASE COUNT         2289 a         2901 c         2851 g         2108 t
ORIGIN      
        1 cgctcccttc tctttgcatt tcttgacacc ttgggcgggg ctatattagc atgcgcgctc
       61 ggacgatagg cgcgtagctc agggggagag cgctaccttg acacggtaga ggtcgacggt
      121 tcaagaccgt ccgcgcctac catctcgccg agggcatctg gccgcctgct gcggttgggg
      181 tgccttttgt atttttgtat cctgctgaga aacaggatgt ttcccgcctc gtgagatttc
      241 ggcgttcaac cggattctca agggaaggtg gtctgcgcct acgagccata gagagaggaa
      301 tgtgccgaaa gtcaccctga agaatgggaa gagttttgac gtccctcctg gttatactat
      361 tgcccaagcc ctggccgctg gcggaattcc tgtcgggccc gaggtgctag ccgcctcggt
      421 caacgggagg ccgaccgatt tgtctgcgcc tttgaccgca gattcgacca tcgatccact
      481 tggattcgac actgccgaag gccgggaaat ctaccggcat agcagcacac acatcatggc
      541 gcaagccgtg aaagaacttt ttccctccgc tcaactgacg atcgggccgg ctttggaaga
      601 cagtttttat tatgattttg cctacgatcg gcccttcacg cctgaagatc tcgagaacat
      661 cgagcaacgg gcgcgcgaaa tcgcgaaacg caagcttgcc gtcacccgcc gcgacctcac
      721 gaagcaagag gcattggagc tctttagatc tcgcggggag cattacaagg tggagttgat
      781 cgaaggcttt ccggatcaag agccgatttc catttatacg cagggtgagt tcgtggacct
      841 ttgccgtggc ccacatgtgc cgtcgaccgg tcacgtgggc gccctgaagc ttttgacgac
      901 ggcaggcgcc tactggcggg gcgatgagcg caacccaatg ctccagagaa tttacggcac
      961 ctcttttcct acgaaggccg agctggatgc tcacctggcc aagctggaag aaatcaaacg
     1021 ccgcgatcat cgcaaagtgg ggaaggaatt agacctgatt tcggtccagg acgagatcgg
     1081 acccgggttg gtgctgtggc atccgaaggg tgccgtcatt cgtctgctga tcgagaattt
     1141 ttggcgcgag cagcatctcc gggacggcta tgagctggtg tattcgcccc acgtggcgcg
     1201 gttggatctg tggaagacca gcggacatgt cgactactat cgcgacaaca tgtttgcgcc
     1261 gatgaagctc gagggcagcg agtatcaact caagccgatg aactgcccct atcacatcat
     1321 gatttacaaa tcacatttac ggagctaccg agagctgcca atccgctatg gcgaattggg
     1381 gacggtgtac cgctacgagc gcaccggcgt gcttcatggc ctgcttcgcg tgcgtggctt
     1441 cactcaggat gacgcgcatt tgttttgccg tcctgatcaa atagaagctg aagtcagccg
     1501 cgtgctggac ttcaccttct tcattttgcg cacctttggg ttcatggaat tcgaagtgtt
     1561 tctttcaact cgtccgaacg aatcagtcgg cgcagaggag cattggacgc tcgcgaccag
     1621 cgcactcgaa gccgcgttga aaggccgcaa catctcgttt catcttgacc ccggcggagg
     1681 cgcattctac ggcccgaaaa tcgacatcaa gatcaaggat gcgctcggcc gatcctggca
     1741 atgctcaacc atccagattg acttcaacaa tccggaacgg ttcgaactca gttacatcgg
     1801 cgaagacgga aagagtcatc aacctatcat gattcaccgc gcgctcatgg ggtccatcga
     1861 gcgatttttc ggcattctca tcgagcacta tgccggcgcc tttccaacat ggctggcacc
     1921 ggtgcaagcg gtggtcatgg cgattacaga caatcagcgt gaattcgtca cggaggtgac
     1981 cgcacacatg aaagcctcag gctttcgcgt tgagtccgac atccggaacg aaaagatcgg
     2041 attcaagatt cgtgaagcgg aaaaggcgaa gataccgttt atgttcgtcg tcggcgaccg
     2101 cgaagtgcag agtcgctcac tgtccgtgag aggtcgtagc ggcgctaacc tcggcacaat
     2161 gaccgtccaa ggcgcgttgg atctgctcaa gactgaagtc gagcgggccg ggcagcaact
     2221 ttcccctaca tcatgagagg tggcttatcg tccccaaact gcgggtgaac cgcgaaattc
     2281 gagtccgtga agtcagagtg attgggccgg aaggtgagca actcggcatt cttccgactc
     2341 ccgaagcttt caagcaggca caagaaggcg gatatgacct ggtggaagtg gccccgacgt
     2401 cggtcccacc ggtctgccgc atcatggatt acgggaagta taagtacgag ctgagcaaaa
     2461 aagatcacca gagccgtcgc caccaaaaat caactcaggt gaaggaaatc aagttgcgtc
     2521 ctcgcaccga taagcatgac ctggagatta agatccgaca gatcaaaggc ttcctggagg
     2581 agggcaacaa gacgaaggtc gtcctgacgt atcgggggcg agaaatggcc aatcaggaaa
     2641 tgggacgggt cgtcatgaac tcagtgatct ctgagctatc cgaagccggt acgatcgagt
     2701 atgcgccgcg gatggaaggg cgcagcttga tcatgatcgt cgctcctaag aattagagag
     2761 gccgaacgct taaccaaagg atgcgaacat gaagatgaag tcacatagcg gagcaagcaa
     2821 acggtttcac agaacaggca cgggcaaatt catgcgcaaa cgcgccggca agcgccatat
     2881 tctgaccagc aagaatcgtg agcggaaacg ccggctgagc ggatcggtgg tcgttgacga
     2941 aaccaaggcc gcggcactca agcgcctact accctataca tagagatgtt aacctgagct
     3001 caacaaggag tatgagtcat gccacgcgca aaaggtgggc caaaaacacg agcaagacgg
     3061 aaaaaacgac tcaagctggc gaaagggcaa tatggcgcaa agagccggct gtttcgctca
     3121 gcgacagaat cggtagataa gggacagcag tatgcctaca ccgggcgcaa gaatcggaag
     3181 cgggacttcc ggcagttgtg gatcgctcgc atcagcgcgg ccacgcgggc gcacgggctc
     3241 acgtacggcc gcttcatcaa tgcgcttaaa aaagccaacg tattgttgga tcgcaaagtg
     3301 ctgtccgata tggcgatcaa ggatgtcgca ggattcgagc agcttgtcgg cctggccaaa
     3361 caacatttaa caccggcgac cgcataactc caccttttcg caactccacc attggagggg
     3421 gacgagcgtc ctcctccgct caatggacct caacgagtcg agagagattg acgtcgaagg
     3481 cggcgaccgg tacggcgatt tgccaccgtt caagcacttc ggggtgaact tctccgatga
     3541 tacccagcgg ctttcccgat accacaatct gccctgcccg gccttccagg aaagacggat
     3601 gttgaaccgg ctcgagagtg tagtcctttc ccatatgata aaacaggatg tccaagcagg
     3661 aatgaatctc cgaaaaatgc gccgccgcat gggcgatcgc cgcacccacg accgtctccg
     3721 tacgtgaacc caattcgtga cgacgatcag gaatcgcgac atcgcccgct tcaaagagac
     3781 gatgggggta aaacgcccgg cctgaagcgg cttccacacg caacagggaa ggcagcatcc
     3841 actggcggag gcaagaaaaa ctcaacgaca taacattttc aacctcgacc atttcacccc
     3901 aaagcgtttc gttcaaccgc atcgcatcgc gatattgctc cggtgatcct agaatattcg
     3961 agatgatctc ttgaaatccc atgcccacca tcagttctcg cgcccggtcg gaggtttgct
     4021 cgattttcga gagcccgcca accgtaaact gtgcgggcat gacgggagag aaatcgccat
     4081 aaccccggct gatcgcgacg tcctcaacga catccatggg atgcatgagg tcctggcggt
     4141 agggcggtaa ctttgccttg accgtaccct ttgacggtgt gaccacatac ccatacacct
     4201 caagggcttg cttgaccgcc tgcgcgccaa tcgtttggcc gagcgcctgt tcaatggtct
     4261 ggatgggaat cgtccgcgtg atatcgaggt cccgaggtgt cacaacctgt tttccgaacg
     4321 gcgtcttcat gggatagcgc acgtcgatcg ggtcaatgac cgctcctcga tcagccagat
     4381 ttgctgcgaa gatattcagg gtcagcacca ccattggcag atcggtcccc gtgacctcca
     4441 cgaacaaggc atcgtcgccc acttgcactt cgccgatctc gcgactgttg ataataggtg
     4501 gaaatgatag cggctgccct atggcgtctc gaagaatcgg caactgatcg tggtcagcta
     4561 agatgtgtcc gtactcgagc cctttcggat ggaccataag aatttcgccc agcgtcatcg
     4621 tcgtctccat gccgagagga gtaaacttcg cggcatccgg tttcacgagt tcatatgtca
     4681 caggaaattg aatacgcgct aagcggtaga gaccgatgga gaccgttttc cgcttgtgcc
     4741 cgaagatttc ggacagtttt tcctgcgtct ggatcagctg cgccaatcct tcctcagtca
     4801 cgcgataccc ggtggaagcg caggcggcaa cgtacgggcg cacccgctcc aggcccggcg
     4861 caacattcag gcgtctgaag gctttcttgg acgtggccga gaagaacggg taggccctga
     4921 gttttcgttg ttgccggatg cggatttgcc gcgcaatccc ttcacaacac cagagatccg
     4981 gccggttgct gtcctgtagc tcaatccgca actctcccgt ctctgcgacg tgctctttca
     5041 gttcgccctt caccagcatc agccattctt cgagctgcgg gatagtcacg ggccgcttcg
     5101 cacgtccgtc cgacgcaagc agtgattcca aatcgctttt ataaattgaa atcgttggca
     5161 tcagctagaa cacgcctcgt gtcgttcgaa tcatatccag attattcgta aagagttcac
     5221 ggatatcgtg tatgcccagc gcgaccatcg ccatacgatc cagaccaagc ccccaggcga
     5281 tgaccggcac cttgacacca agcggcaggg tgacttccgg cctgaacagt ccagcaccgc
     5341 cgagttccat ccaaccaaga cgcggatgac ggacatgcaa ttccactgag ggctcggtaa
     5401 acgggaaata cgcgggcaaa aactttacgt ctttggcctg cgccacttct cgggcaaaca
     5461 gattcaacaa cccgagcagt gtgcggaagt tgatatcgtc gccgaggaca atcccttcga
     5521 cctgaaagaa atctgtggca tgggtcgcat caacctggtc gtaacgaaag caacgcgcaa
     5581 tggaaaaata tttggccggt gtggccggcc cagacgccag ggttctcgca gacaccgccg
     5641 taccttggct cctcaatacc agccgcttcg cccgttcgac gtcaaaggcg taccgccatc
     5701 ccatggaccc ggtatttccg ccgtctcgat gagcctgagc gacgcgagaa aggaatggtt
     5761 cagcaacatg cgtggcacag gtgggatgtt tgacgaaata cacatcgtgg atgtcgcggg
     5821 ccggatggaa ctgcggcatg aagagtgcgt ccatattcca gaattcggtc tcgaccagcg
     5881 cgccccgcat ttcccgaaag cccatgctca ccagcttcga tttgacggtg tccaaaaact
     5941 cacgataggg atgtcttttt cctgtgctga tccgtggtgc gcgaaggcta atagtgtatt
     6001 tcctgaaccg ctttgcacgc caactgccgt ccttcagcat ttcgggactg agttgggaga
     6061 cttcttccgc agcaacgtca tgttgcacca gatgctccgc tgccgcgagg ccggctggcg
     6121 ccactctgaa agatcgagcc acacggtcgt cgacgcgaaa cggttctttc gcgttgcccc
     6181 gcctcacggc gtactcttgg ataatctgcc gatcgggttc tgcgaaactt tccaattcgc
     6241 gaggaccttc atgcacctgc tgcagtaagg ttctcacccg atcggccgtt gggctcgggc
     6301 gtccggtcga ctcaatgcaa cctccctgca caatcagcag cgccccttcc tttttcaggc
     6361 ggccgacggc cttgctgaca tcggatggat cgagaccttc ttgagattgc aggtcccgga
     6421 tcgtcaaccg gccgccggtt cccgctgcac tcctcgccgc cgccaacact tcttcgatcg
     6481 gggcttcctt cccatgataa cgctcgccga ccggtgtcag tgagacgact ggggtgatgg
     6541 tttccttgtc gacagcgagc agcgatttgg cgatcaacca ttcgatggcc atgctgagtt
     6601 gcgaggcttc aagatttgtg gctttggcga gttgatcggt gtcgagcacc agccctgatg
     6661 gattggtgcc gaaggctgtg agcaccttga tttccagtgg gtgcaggctg tcgatgaggg
     6721 aggaaatgtc cacccgatgg cctatcgatg gacggatgca ggctgccgag ccgcccggag
     6781 agcagcgatc gtcaccgcat aatcgtctcc gcagaaaatt gcggaaccag cgaccaacac
     6841 gtctgcaccg gcggcgagga ttcgagccgc gttgtcaact ttgacgccgc catcgacctc
     6901 cagcagcgca cgactcccga taccgtcgag catgcgccgt gctgtgctca ccttgtggag
     6961 gcaggattcg atgaacgtct gtccaccgaa tccgggattc acggacatga taaggagcag
     7021 atcgatgtcc ggaagtattt cttcgatcgt gtgcaatgag gttgcgggat tcaatgtgac
     7081 acccgccttg acaccgcgct ccttgacggc ttgtacggtc cggtggagat gtgggcaggc
     7141 ttcgacatgg acggtgaggt aatcggcacc agccgaggca aagtcggcga gaaaggcatc
     7201 ggcattcgta atcatcaggt gaacgtcaag gggaagcttg gtcactttct tcaaggattc
     7261 aacgatcggc ggacccaccg taagattcgg cacgaaatgg ccatccatga cgtcaacatg
     7321 cagcagatct gctccacctc gttcgaccgc ggccacttcg tcggccagac gggcaaagtc
     7381 ggcagagaga atagaaggag cgatgagcgc ggttctggcg gtcatgaatc cattcgtgtc
     7441 aagcgtgccg cataaaagcc gtccatcgaa tccacgtttc ccatcgtcga gaaatcgcct
     7501 cgtgcagtga ggaattgccg gccgggttcc ggcagccagg gcgccacgga cattcgacgg
     7561 aactccgcat gcgacctgag gaatccatcg ataacaactt cgttttcctc cggctctgtt
     7621 gaacacgtac tatagaccag caccccgccg ggtcgcaagc agcgagccgc cgaggcgagt
     7681 atgtgcgatt gcacggcctg atgacgtgcg aaggcattct ggccctttcg ccatttcgac
     7741 tcgggatgcc gtcgtaacac gcctagaccg ctgcatgggg catcgacaag aattcgatcg
     7801 aaccataggc gaccgggcgt tttgaccatt ttagctgctg cgtcttccca cgaagagtct
     7861 ctcaggtcgc caacgatcgg cacaatgctc ttcaattgta atcggctgct atttatctgc
     7921 acggcgcgga gtctgccagc actgcgatcg actgcgtaaa tccgtccctg gtcctccatg
     7981 agttcggcta tatgcgtcgc cttccctcca ggcgccgcgc atgcatccag gaccaggttg
     8041 ccgggacgta cgtcgagaag gaaagggatc aactgagcag cctcgtcttc gacatagaag
     8101 tgaccctctg caaaacctgg caacatcgcc acgcttcccc cttccacaag cgtgatgcct
     8161 gccgggctta ttcgtgtcgg ctttccttga atgccggcct tctccatcct acgaagcaat
     8221 tcctcccgag tgatccgcag gagattcacg cgaagcgtca ggggagggat caggctcgcc
     8281 tgttcgcaga cagtttgagc aacggcgagg ccgagccgat cgagccagcg acgactcagc
     8341 cattcgggaa cagaataccg tacggcgagt gcgtacgcgg catcgtcgtg gactgacggc
     8401 cattgcacga tcggctcacg aatcagcgca cgcaagacgg cattgacgaa accaccccaa
     8461 tctcggtgca gcttcttcac ctgggccttg gcgagattca ccgtttcatt gacggcagcg
     8521 gaagcgggga ttcgatcgag aaagagcacc tgataggcac ccatccttaa caacatctgg
     8581 acggctattg gcagacgggc cagcggctta tcaagaaccg gttctaaccg ccaatcgatc
     8641 gtgagcaagc gacggcagac cccgtaggtg atttcaaggg ccagcgcgcg gtcgcggcca
     8701 tcaagcggtg ttcggagcag cgcacgatcg aacagtgtat cgaggccttc cgtagaatgc
     8761 gccgattcaa ccagcaccga caacgcaacc gacctggccg aatggatagg catgccaatt
     8821 gagacagtgg ccacttagca gaaatcaccg cgggaactgc agagcacaag ttatcaacgc
     8881 tctagcatga gcggcgatgg catgccaggg gctgacttgc attcactgat gaaagtgtcg
     8941 agcgtttact gtgtgtgagc gcccagtcgc atgccggcac cgatcggatg gcccgtcaga
     9001 tactgcgcca ccggcattcg acggcccccg gccgcctgaa cttcgtagat cgccagtact
     9061 cctcgacctg tggctatatg gagcgcatct ttttggactg ctgtcacctc accgggaact
     9121 cctctcgccg gctcgtccaa gaccacggca cggtaaatcg tccagcgctc tgttttcgcg
     9181 aaagtatagg caccgggcca tggatcgagt ccgcggatac gattggcaat ggcagtcgca
     9241 ggcatcgtcc aatcgattat gccatcttct ttctttaaca gtggagccag agtcgcttgg
     9301 ctatgatctt gagctcgagg aatcagagtg ccggctctta gctgcgccag agtcttcaca
     9361 agcagttgac ctccgagctc agcgagacga acagacaagc tgccggcagt atcttcggag
     9421 ttgatcgtca ttgcttcttg gagcaacacg gctcccgtgt ccatgccctc atccatcaac
     9481 atggtagtaa tgccggtttc ggtttcccca ttgatgaggg cccattgaat cggtcctgct
     9541 ccccggtact tcggcaacag tgatccgtgt acattgatac agccaaaggg cggaagggac
     9601 aggattgcgg gaggcagaat acggccgaac gctgcgaccg caatatagtc gggcttccag
     9661 ctggataatg tcgttataaa gtctccatcc ttcattttag tcggttgcag aagcggtatg
     9721 tgctcacgtt gtgcgatgag tttcaccgga gagggcgtta atacttggcc gcgccctttc
     9781 ggacgatcgg gctgcgtcac aatgcccacc acctgatcgg gccctttgag cagcgcctca
     9841 agtgatggaa cggcgaattc cggtgtcccc atgaacacaa tgcgcatggc accgctttag
     9901 cattcgtggg ccccgaaagc aacgggacgc atcgtttgac ttgccgagaa tcgctttgtt
     9961 agtatccgcg cgcggggtgg agcagtctgg tagctcgttg ggctcataac ccaaaggtca
    10021 gaggttcaaa tcctctcccc gcaaccaacc atctcaagca cttagcgatc cagaaacaac
    10081 ttagccggag agaaaacggt ggtattgtcc gtgttctgtc cgtgttccct cgttaactgc
    10141 tgcatgcgg
//
LOCUS       DASSCQ010000093         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_158607, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000093 DASSCQ010000000
VERSION     DASSCQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158607"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFH05_06535"
     CDS             complement(<1..384)
                     /locus_tag="VFH05_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677903.1"
                     /translation="MNARRKKLIALVVLGGLWAGLILWECLSWEQPVRVPLANVSGSG
                     GAIRQAHSGTNGLHVNLNLLAAARTQREMVFATPRNIFALPSASVANQPAPLEASDRA
                     LRQQSIAAELSQFHYVGFVRTGEQWE"
     gene            complement(394..951)
                     /gene="pilO"
                     /locus_tag="VFH05_06540"
     CDS             complement(394..951)
                     /gene="pilO"
                     /locus_tag="VFH05_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET6677904.1"
                     /translation="MKRYLVEQLRQPYGPLLPQAAVVLILLLVLWAVKSLGVEAAEQS
                     RSSLTTEWARVRKEHQHHQAALRAKQDLAQVWAALPEERDFAPLALGITEEAKRNHVT
                     LPALSYKTESTPVLHTSKGLLQGTMSGRYEDLRRFLYDIETADELVYIEDVDLVPSAT
                     QQDNLTFNVKIVTYLRNEPQKPTVP"
     gene            complement(948..1586)
                     /locus_tag="VFH05_06545"
     CDS             complement(948..1586)
                     /locus_tag="VFH05_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET6677905.1"
                     /translation="MRRRISIITSIADRIGSFRWQTGGTKYFHLELASRHRPYLAPLR
                     VLLKATTLGLIVWACWSTAEALLAFRGLHETEARLNQARAQDQQLIDEARAAGIDLSE
                     AALRQLPGEVALANQLLTKRNFSWTQFLSGLEEAIPPRVSIKSVRLDPASAVIHMTGA
                     AVTVEDVTGFTLKLQSHPVFHDPVLGQHHLGGDGLVEFDLMLKYRPPERERA"
     gene            complement(1590..2588)
                     /locus_tag="VFH05_06550"
     CDS             complement(1590..2588)
                     /locus_tag="VFH05_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677906.1"
                     /translation="MWGMGDSRPTECLKIGGQALVWGEASKTWRGRHRYRCALSPLPP
                     GVIKLSPLEGNVIDHAAVQERLRTLAGPARRMRWLGRTLLSGLPRPVTLVLPDLAVRS
                     TVLQLEQIPARKQEQEALIRWRLGQEQRVPLTATRLRWQVFPPRQAKTGANMVLVIAV
                     QEGILAEYEAACEAVGLLPREVTVTSFRLFDLWLKAAGGARCLNGDLAWITVLDGGLT
                     CLIIHDGRPVFCRTKFLASDTGQAQDADSGAGMKRVVRDTELSILACREHFPDLQLSR
                     LVMMTDSDIPGLEDALGQDLGVSTDQLQWNHVETVGWTSLGGSSSMAALPVVAGLM"
     gene            complement(2591..>2838)
                     /locus_tag="VFH05_06555"
     CDS             complement(2591..>2838)
                     /locus_tag="VFH05_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HET6677907.1"
                     /translation="QGTGCHECHGTGYKGRKCITEFLDLTDEIKEMILSERPLSEIRY
                     RAVTGGMITLRQSALRKVIEGGTTLREINRVTFSEEA"
BASE COUNT          581 a          864 c          799 g          594 t
ORIGIN      
        1 ctcccattgc tctcctgtcc gaacgaaccc cacgtagtgg aattgcgaca gctcggcggc
       61 gatggattgc tggcgtaagg cgcggtcaga cgcctccaac ggtgctggct gattcgccac
      121 cgacgcggag ggcaaagcaa aaatgttcct gggggtggca aacaccattt ctcgctgggt
      181 gcgagcggct gcaagcagat tcaggttgac gtggaggcca ttggtccctg agtgggcttg
      241 gcggatggca cctcctgaac cggagacatt cgccagcggc acacgaacag gctgctccca
      301 cgacagacac tcccatagaa tgaggcccgc ccagagaccg ccgagcacga ctaaggcgat
      361 gagttttttt cgtctggcat tcatcgtgtc cagctagggg acggtcggct tctgtggctc
      421 gttccgtaaa taggtgacaa tcttgacgtt gaaggtaaga ttatcctgct gagttgctga
      481 cggaaccaga tccacatcct cgatgtatac cagttcatca gctgtctcga tatcgtacaa
      541 gaaccgccga aggtcttcat agcggccgct catcgtgcct tgtaacagcc ctttgcttgt
      601 atgaaggaca ggggtggatt ccgtcttgta agagagcgca ggcaatgtga cgtgattccg
      661 cttggcctct tcagtgatgc cgagtgccag cggtgcaaaa tcgcgctcct ccggcaaggc
      721 tgcccacact tgcgccaaat cctgcttcgc tcgcagggcc gcttggtgat gctggtgctc
      781 tttacggaca cgcgcccatt cggttgtcaa actggaacgg ctctgttctg cagcctccac
      841 acctagtgac ttcaccgccc agaggacgag gagcaagatc aggacgacgg ccgcctgggg
      901 aagcagcgga ccgtaaggct gtcgtaattg ctcgaccaga tatcgtttca tgcccgctcc
      961 cgctccggcg gtcgatattt cagcatgaga tcgaattcca ccaggccatc gccacccaga
     1021 tgatgctgtc cgaggacggg atcatgaaac acagggtgcg actgcaattt gagggtgaat
     1081 cccgtcacgt cttcgaccgt gactgccgct ccggtcatat ggatcaccgc gctcgccggg
     1141 tccaggcgaa cgctcttgat tgagacacga ggagggatcg cctcttcgag tcccgagagg
     1201 aattgcgtcc aagaaaaatt gcgcttggtc agcaactggt tcgccaacgc cacctctcct
     1261 ggtagctgcc gtaaggcggc ctcggataga tcgatccctg ctgcgcgcgc ctcgtcgatg
     1321 agctgctggt cttgcgcccg ggcttgattc agacgggctt ccgtttcgtg caaaccccga
     1381 aaagccaaca gcgcttcagc ggtactccaa caggcccaca cgataagtcc aagcgtcgtc
     1441 gccttcaaca gcactctgag aggagccaga tagggccgat ggcggctcgc cagctccaag
     1501 tgaaaatact tcgtcccgcc ggtctgccaa cgaaagctgc cgattctatc ggcgatgctc
     1561 gttatgatgg agatgcgtcg ccgcatgcct tacatcagcc ccgccaccac cggtaatgca
     1621 gccatcgagg aactgcctcc cagagaagtc catcctacgg tttcgacatg attccactgt
     1681 aattgatcgg tcgacacgcc aaggtcttgt cccaaggcat cttccaatcc ggggatatca
     1741 ctgtcggtca tcatcaccaa ccgggaaagc tgcagatcgg gaaaatgctc gcggcaggcg
     1801 aggatggaga gttctgtgtc gcggacgact cttttcattc ccgccccgct gtcggcgtct
     1861 tgtgcctgcc ccgtgtcgct cgccaggaat ttggtcctac agaacacggg ccgcccgtcg
     1921 tggatgatca agcaggtcaa tccgccgtct aacaccgtga tccaggccag gtccccgttt
     1981 aagcatcgtg cgccacccgc tgctttaagc cagagatcga ataggcgaaa actcgtgacc
     2041 gtcacttctc gtggaagcaa accgaccgct tcgcacgcgg cctcatactc agcaaggatg
     2101 ccctcctgga cggcaatgac caggaccatg ttggcgcccg tcttggcttg ccgcggagga
     2161 aagacttgcc agcgaagtct tgtggccgtc aagggaacgc gctgctcctg gccaagtcgc
     2221 caccgaatga gggcctcttg ctcctgtttt cgagcaggga tctgttcgag ttggaggacg
     2281 gtcgagcgca cggccagatc cggcaagacg agcgtgaccg gtcgcggaag cccggagagc
     2341 agcgtgcgac ccagccaacg catccgcctt gctggtcctg caagagtccg cagccgttcc
     2401 tgcaccgctg cgtgatcgat cacgttacct tcaagtggcg aaagcttgat gacaccgggc
     2461 ggcagcggtg acagggcaca acgatagcga tgccgtcctc gccacgtttt cgaggcttcc
     2521 ccccacacga gggcctgtcc tcctatcttg aggcattctg tcggacggct gtcacccatt
     2581 ccccacatgc ttacgcttcc tcactgaacg tgacgcgatt aatttctcgc aaggtcgttc
     2641 cgccttcaat gactttgcgt aaggccgact gccgcagcgt gatcatgcct ccggtcaccg
     2701 ctcgatagcg aatctcggag agcggtcgct cggacaaaat catctctttg atctcatcag
     2761 tgagatccag aaattccgtg atgcattttc ggcccttata gccggtacca tggcattcat
     2821 gacagccggt cccttgat
//
LOCUS       DASSCQ010000094         7119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_273096, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000094 DASSCQ010000000
VERSION     DASSCQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273096"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1373)
                     /locus_tag="VFH05_06560"
     CDS             complement(348..1373)
                     /locus_tag="VFH05_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET6677908.1"
                     /translation="MRRSKTKLGSKGTSAAADKPNGGSLAEGRRVLRIEARAVQALIE
                     RLDDQFDKAVDLLFRCKGKVVVSGMGKSGLVGQKIAATMASTGTPAFFLHPADGIHGD
                     LGMVGRADVLIAISNSGETQEILQLLPYVERLGIPVVSLTGRVNSTLAKNSDATLDVS
                     VAEEACPMGLAPTASTTATLAMGDALAVVLLQKRGFKQDDFAQFHPGGTLGRRLLVRV
                     RDLMHGGADMPVVAESVPATAAILEITAKKLGMTTVIDAGGKLIGIITDGDLRRFIHK
                     GGDFAKVTAGDLASRNPKVIKPDDLAAKAVEQMERFSITTLVVAESDRKMFGVIHLHD
                     LLKNGIV"
     gene            complement(1349..2173)
                     /gene="kdsA"
                     /locus_tag="VFH05_06565"
     CDS             complement(1349..2173)
                     /gene="kdsA"
                     /locus_tag="VFH05_06565"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET6677909.1"
                     /translation="MAHLVDIGTFTVGQGQRPFLIAGPCVIESEQLVIETAGHIAEIA
                     RALGMPYVFKSSFDKANRTSITSFRGPGLERGLEVLAKVKKQIGVPVLTDVHTETQAT
                     QAGRVVDVLQIPAFLCRQTDLLIAAAKTGKVVNVKKGQFLSPLEMGNAVKKVEECGNR
                     RIVLTERGSSFGYNNLVVDMRAFPILRSFGYPVVFDATHSVQLPGGGGTQSSGQREFV
                     EPLASAAAGAGVDGFFMEVHPNPNEALSDGPNMVPLHQLKSLLERVMRICDAAKQS"
     gene            complement(2351..3955)
                     /locus_tag="VFH05_06570"
     CDS             complement(2351..3955)
                     /locus_tag="VFH05_06570"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6677910.1"
                     /translation="MMSKYIFVTGGVVSSLGKGLASASIGNLMESRGLKITFLKLDPY
                     INVDPGTMNPYQHGEVYVTDDGAETDLDLGHYERYTSLTLTKENNYTTGRIYNSVITK
                     ERRGDYLGGTVQVVPHITDEIKQCIMRISQGMDVTIVEIGGTVGDIESLPFLEAIRQM
                     PYDVGRENVLYVHLTLVPYIGAAGELKTKPTQHSVNKLREIGIQPNILLCRTDRYIPP
                     EMKAKIAMFCNVEKDAVITAKDVETIYEVPIVFRKEGLDELIVRLLKLETGPPNLREW
                     DAMVQKIKHPKHEIAVALVGKYAGLKECYKSLAEALVHGGIDHETRVNITWIESEDIE
                     RQGTERILREADGILIPGGFGSRGIEGKITTIRYARERQVPFLGLCLGMQCATIEFAR
                     HVAGLAGANSAEFDERSPHPVIHLMQDQQSISDKGGTMRLGSYLCRLAEGTLAHKMYG
                     VNEVRERHRHRFEFNNSYREQLTAKGFVLSGLSPDGRLVEIVELKDHPWFLATQFHPE
                     YSSRPHRPHPLFSGFVGAALRKKLGH"
     gene            complement(3999..4784)
                     /gene="kdsB"
                     /locus_tag="VFH05_06575"
     CDS             complement(3999..4784)
                     /gene="kdsB"
                     /locus_tag="VFH05_06575"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET6677911.1"
                     /translation="MSKQKPQVTVVIPARYGSSRFPGKPIAMLGQKPIIQHVYEQAAA
                     CRAVSEVVVATDDRRIQEAVVKFGGQAVLTTGDFRSGTDRVAAVAGLRTGQFILNLQG
                     DEIPVDPDLLTDLIEPFVESGAEMGTLKRTLHTAEDLQNPAVVKVVTDRHGRALYFSR
                     APIPLVRDDPSRQAVKGLHYIHLGVYIYTRETLLRLTALPTGMLEDSEKLEQLRALDH
                     GITIHVWETGRESLRIDTPADATTAGEALRRLTLDKLETLNQL"
     gene            complement(4777..5841)
                     /gene="rfaE1"
                     /locus_tag="VFH05_06580"
     CDS             complement(4777..5841)
                     /gene="rfaE1"
                     /locus_tag="VFH05_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET6677912.1"
                     /translation="MGAVREGDSVNKPARDSTGAAGSSVRSDKPSASSLRRHIQRFPQ
                     ASVLVIGDLILDHYIWGRVSRISPEAPVPVVHVESESMRLGGAANVFNNILSLGGRAD
                     ICGIIGSDEAGRLLLKELGTKRSGHRGVVVDGNRPTIKKSRVIAHNQQVVRYDVERKG
                     ELKPQLQKKMLRYVESRLREVTCLVVSDYAKGVVTAGFMADLVRLAALRKVPVIVDPK
                     VEHFGFYKGVTVITPNHLEATQAAGLHGDGDQTIQEAGETIRQRLGCQAVLVTRGERG
                     MSLFEGNKDSWHIPTQARQVYDVTGAGDTVVGTLALALSIGVSMREAAMLANYAAGVV
                     VGMVGTATVTTKQLSETLGE"
     gene            complement(5811..6491)
                     /gene="lepB"
                     /locus_tag="VFH05_06585"
     CDS             complement(5811..6491)
                     /gene="lepB"
                     /locus_tag="VFH05_06585"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6677913.1"
                     /translation="MSTSVQPIRKSVFREYAEAIVVAMLLAFAIRVFVVQAFKIPSGS
                     MIPTLLIGDHILVSKLAYGIQWPSDCKFQVAFPPVNCYASHALIEFGKPQRGDIIVFR
                     FPEDEEKDFIKRIVGTPGDTVQLKNKVVVVNGTPLDDKAFTQRIDSGVIDGTINPRDN
                     FGPVTVPPDSYFVMGDNRDQSLDSRFWGFVREEKIRGKAFRIYWSWSGQGQWQEWVRW
                     GRFGKAIQ"
     gene            complement(6563..>7119)
                     /locus_tag="VFH05_06590"
     CDS             complement(6563..>7119)
                     /locus_tag="VFH05_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET6677914.1"
                     /translation="MGAILQLCQERRGTQRSIHFLDPTRVLISYEMPLNEVILDFYDK
                     LKSRTQGYASLDYELLGYRESDLVKLDILLNGEAVDALSFITHKERSYPRGRQLAEKM
                     KELIPRQMFEIAIQAAIGNKVIARETIGAMKKNVTAKCYGGDITRKRKLLEKQKEGKK
                     RMKAVGSVEVPQEAFLAILKVGEE"
BASE COUNT         1463 a         2183 c         1903 g         1570 t
ORIGIN      
        1 gcgctcaacg aacggtccgg cgtgggggta aaaaacagca tcacgaacac aggaatcagc
       61 aaaatacgca cgagcgtgat gaaattgggc aaattcaagg actcttgccc gaccgccttc
      121 catgcttcaa ggacacgatt catggcgatg tagttgctcc ctctttcttt ttgatagaat
      181 gaacacgttc acattgaagc gggtgtattt ctcacccgcc caccctcgcg gcgccgagac
      241 gccgcgtctg cccacatctc acccacccac ctggtgattc cggttgcaga tttcatccgt
      301 atcttcgagc acgcgaatac gatgcagggc gctggtgcat ggttcagcta cacgatgccg
      361 ttcttgagca aatcatgcag gtgaatcacg ccgaacatct tccgatcgct ctcggcaacc
      421 accaaggtgg taatggaaaa ccgctccatt tgttcaacgg ctttcgccgc aagatcatca
      481 ggcttgatga ctttcgggtt acgcgaggcc agatctcccg cggtgacttt cgcaaagtcg
      541 ccgcccttat ggataaaccg tcttagatct ccgtcggtga tgatgccgat gagcttgccg
      601 ccggcgtcga tgaccgtcgt catgcccagt tttttcgccg tgatctccag aattgccgcg
      661 gtggccggca ccgattcagc caccaccggc atgtccgctc ccccatgcat gagatctcgt
      721 acgcgcacga gcagtcgccg gcctagcgta ccgccgggat gaaattgcgc aaaatcgtcc
      781 tgcttaaacc cacgtttctg caaaaggacc acggcaagcg catctcccat ggccaacgtt
      841 gccgtcgtgc tggccgtagg ggccagcccc atgggacagg cttcctcggc aacggacaca
      901 tcaagagtcg catcgctgtt tttggccaga gtggaattca cgcgccccgt cagcgagacc
      961 accggaatac cgagccgttc gacgtatggc agtaactgta atatctcctg cgtctcgccg
     1021 ctgttggaaa tggcgatcaa cacgtcggcc cggcccacca taccaaggtc tccatggatg
     1081 ccgtcggcgg ggtgcagaaa aaaggcgggt gtacccgtgc ttgccattgt ggccgcaatc
     1141 ttttgtccca cgagtcccga cttgcccatc cccgacacca ctaccttgcc cttgcaacga
     1201 aacagcagat cgacggcttt gtcgaattga tcgtccagtc gctcgatgag ggcctgtacg
     1261 gcgcgcgctt cgatccgcag gacgcgccgg ccttcagcga gactcccccc gttcggcttg
     1321 tcggccgctg ctgacgttcc tttgctgcct agctttgttt tgctgcgtcg catatccgca
     1381 tcactcgttc gagaagcgat tttagttgat ggagcggcac catgtttggt ccatccgaga
     1441 gggcttcatt tggattcgga tgcacttcca tgaaaaatcc gtcgacgccg gcgccggccg
     1501 ccgcgctggc cagcggctcc acgaactcgc gctgtccgct cgattgtgtc ccgcccccac
     1561 ccggcagttg cacgctgtgc gttgcgtcga acaccaccgg atacccgaaa ctgcggagga
     1621 tcgggaaggc ccgcatatcc acgaccaggt tgttgtagcc gaacgatgat ccacgctcag
     1681 tcaacacgat gcgtctgttt ccacactcct cgaccttctt gaccgcgttc cccatttcaa
     1741 gcggcgacaa aaactgcccc ttttttacat tcacgacttt gcctgtcttt gctgcggcaa
     1801 tcaacaaatc cgtctgccgg cagaggaacg ccggtatctg cagcacatcc acaacacggc
     1861 cggcttgggt tgcctgggtc tccgtgtgca cgtcggttaa caccggcacc ccgatctgtt
     1921 ttttcacctt tgccaacacc tcaaggcccc gctccagtcc cggtccgcga aacgacgtga
     1981 tggaagtccg attggccttg tcgaacgacg acttgaacac gtagggcatg ccaagcgctc
     2041 tggcgatttc agcaatgtga ccggctgttt cgatcaccaa ctgctcgctt tcaataacac
     2101 agggaccggc gatcaaaaaa ggacgctgcc cctgaccgac cgtgaaggtg ccgatgtcta
     2161 caagatgcgc catggcgatt aactatcgtt ttcccctgag atactcaaac agcaagccat
     2221 tttcacccgc ccagccctgg ctcgcgagag cgggctcttc ccagtctcac ctgcccaacc
     2281 cgactcgcag gagcgttgag gaaccttgtc gagccgagaa cgaagttgga acctgtttca
     2341 gcatcccagc tcaatgtccg agcttcttgc gcaatgccgc tccgacaaac ccgctgaata
     2401 gtggatgcgg gcggtgcgga cgcgaactat attcgggatg aaactgtgtg gccaaaaacc
     2461 aggggtgatc tttcaactcg acgatttcga ccaaccgccc gtccggcgag agcccgctca
     2521 acacgaatcc tttggccgtc aactgctcac ggtaggagtt attgaactca aagcgatgcc
     2581 gatgtcgttc acgcacttcg ttgacgccat acattttatg cgcgagcgtc ccctcagcca
     2641 gccgacagag gtagctcccc agccgcatgg tgccgccctt atcgcttatg ctctgttgat
     2701 cctgcatcag atgaatgacg ggatgaggcg accgctcgtc gaattcagcg ctgttggcgc
     2761 cggccaagcc tgccacatga cgggcaaact cgatggtcgc gcactgcatg cccagacaga
     2821 gtcccagaaa cggaacctgg cgttcccggg catagcggat cgtggtgatt ttgccctcga
     2881 tcccgcgtga gccgaagccg ccgggaataa gaatgccatc cgcctctcgg agaatccgtt
     2941 cggtcccttg ccgttcgata tcctccgact cgatccatgt aatattcaca cgcgtctcat
     3001 ggtcgattcc cccgtgaacg agggcctcag ccaggctctt gtaacattcc ttgaggccgg
     3061 cgtacttccc gacgagagcc actgcaattt cgtgcttggg atgtttgatc ttctggacca
     3121 tggcatccca ttcacggaga ttcggcggcc ccgtttctaa cttgagcagc cggacgatga
     3181 gttcatccaa gccttccttg cggaacacga tcggcacttc gtaaatggtt tcgacgtcct
     3241 ttgcggtgat gacggcgtct ttctccacgt tgcagaacat ggcgatcttc gccttcatct
     3301 ccggcgggat gtaccggtcc gtgcggcaca gcaaaatatt cggctggata ccgatttcgc
     3361 gcaacttgtt caccgaatgc tgcgtcggct tggtcttcaa ctcgcctgcc gccccgatgt
     3421 acgggaccag tgtcagatgg acgtacagga cgttttcacg gccgacatcg tagggcatct
     3481 ggcgaatcgc ttcgagaaac ggcagacttt cgatgtcgcc gaccgtaccg ccgatttcca
     3541 cgatggtcac gtccatccct tgggagatcc gcatgatgca ctgcttgatc tcgtcggtaa
     3601 tgtgcggcac aacttggacc gtcccgccaa gatagtcgcc acgccgttcc ttcgtgatga
     3661 cggaattgta gatacggccg gtggtgtagt tattttcttt cgtcaacgtg agggaggtgt
     3721 accgttcgta atgaccaaga tccaagtctg tctctgcgcc atcgtcggtg acatacacct
     3781 cgccgtgctg atacgggttc atcgtgccgg gatcgacgtt gatataagga tcaagtttga
     3841 gaaacgtgat cttgagtccg cggctttcca ttagattgcc gatcgacgcc gaggccaacc
     3901 ccttcccgag agacgacacg accccgccgg tgacgaaaat gtatttgctc atcatggtct
     3961 ccatccgtgg cgatcgagcg aaccgaatcc gcattcagtc ataactggtt caatgtttca
     4021 agcttgtcga gcgtcagtcg tcgcaaggct tcaccggccg tggtcgcgtc ggccggagta
     4081 tcgatgcgca aggattcacg acccgtttcc cagacatgga tggtgatgcc atgatccaga
     4141 gcacgcaatt gctccaattt ctcagagtct tccagcatcc cggtcggcaa agcggtcagc
     4201 ctgagcagtg tctcgcgggt atagatgtag acccccaagt ggatatagtg caaccccttc
     4261 acggcctgcc gggagggatc gtcgcggacc aagggaatcg gagcacgtga aaagtacagg
     4321 gcgcggccat gccgatcggt aactaccttg accacggcag ggttctgcag gtcctcagcg
     4381 gtgtggagcg tacgcttcaa cgtgcccatc tcggcgccgg actcgacgaa gggttcgatc
     4441 aagtccgtga ggaggtcggg atcgaccgga atctcgtcgc cttgcagatt caggataaac
     4501 tgaccagtgc gtagcccggc cacggcagcg acacgatccg tcccgctccg aaaatcgccc
     4561 gtggttaaca ccgcctgtcc accgaatttc acgactgcct cttgaatccg acgatcgtcg
     4621 gtggcgacga cgacctcaga gaccgcccgg cacgccgccg cttgttcata gacatgttgg
     4681 ataatcggtt tttgcccaag catggcaatc ggtttccccg gaaaccggga ggagccgtac
     4741 cgggcgggaa tcaccaccgt gacctggggc ttctgcttac tcaccaagtg tctccgataa
     4801 ctgcttcgtc gtcacggtcg cagttccaac catgccgacc acgacccccg cagcatagtt
     4861 ggccaacatt gcggcctctc gcatcgaaac gccgattgac aatgccaacg cgagggtccc
     4921 cacgaccgta tcgccggcac ccgtcacatc atagacctgg cgcgcctgcg tggggatatg
     4981 ccatgaatcc ttgttgcctt cgaacagact catgcccctt tcacctcgcg tgacgagcac
     5041 cgcctggcac cccaagcgct ggcgaatggt ttctccggcc tcttgaatcg tttgatcgcc
     5101 atcaccatgg agtccggcag cctgagtcgc ttccagatgg ttgggcgtga tcaccgtcac
     5161 ccctttgtaa aagccgaaat gctcgacctt cggatcgaca atgacgggga ccttcctcag
     5221 cgctgcgagc cgaacgaggt ccgccatgaa tcctgccgtc acgacccctt tcgcatagtc
     5281 ggacaccacc aaacaggtga cttcgcggag acgagattcc acataccgca acatcttttt
     5341 ttgcaattgc ggcttgagct cgcccttgcg ttcgacgtca tagcggacga cttgctgatt
     5401 atgggcgatc acccgggact ttttgatcgt gggccggttc ccatcgacga cgacgccgcg
     5461 atgcccggag cgcttcgtcc ccagttcctt caacagcagg cgtccagctt catccgagcc
     5521 gatgatgccg cagatgtcgg cccggccgcc caatgacaga atattgttga agacattggc
     5581 ggctccgccg agtcgcatgg attccgattc gacatgaacc acggggaccg gcgcctcggg
     5641 ggaaatccgg ctcacgcgtc cccaaatgta atgatcaagg ataagatcgc cgatgacgag
     5701 gactgaagct tgtggaaatc gttggatatg ccgacgaaga ctgctggccg acggcttgtc
     5761 agagcggact gaagatcccg ctgcgccggt cgagtcccgc gccggcttgt ttactgaatc
     5821 gccttcccga accgccccca tcttacccac tcctgccatt gtccttgccc gctccaggac
     5881 cagtaaatgc ggaaggcctt ccccctgatt ttttcctcgc gaacaaatcc ccaaaaccgg
     5941 ctgtccagac tttgatctcg attgtctccc atgacgaaat aggaatcggg cggcaccgtc
     6001 accgggccga agttatcgcg cggattgatc gtgccgtcga tcacccctga atcgatgcgc
     6061 tgggtaaacg ccttgtcgtc cagcggcgtt ccgttgacca ccaccacttt gttcttcagc
     6121 tggaccgtat ctccaggagt gccgacaatc cgtttgatga aatccttttc ttcatcttcc
     6181 gggaaccgga agacgatgat gtcaccccgc tgcggtttcc cgaactcaat gagtgcatga
     6241 gaggcgtaac aattcaccgg aggaaaggcc acttggaatt tgcagtcgct cggccattgg
     6301 atgccatagg ccagtttgct gaccaggatg tgatcgccga tcaagagcgt ggggatcatc
     6361 gaaccggagg gaatcttgaa tgcctggacg acgaagactc ggatcgcaaa ggcgagcagc
     6421 atcgcgacga cgatggcttc ggcgtattca cggaagacgg acttgcggat gggctgcacg
     6481 ctggttgaca cctctactcc tgtcgtggcg tccgatgcac gggacgcgag ctcgtcggag
     6541 ctgggaagat tcggatcgac gctcattctt caccgacttt caagatcgcg agaaaggctt
     6601 cctgcggaac ttcaacgctg ccgacggctt tcatccgttt ctttccttct ttttgttttt
     6661 cgagcagttt gcgcttgcgc gtaatgtcac cgccataaca ctttgccgtg acgttttttt
     6721 tcatcgcgcc gatcgtctca cgtgcaatga ccttgttgcc gatcgccgct tgaatcgcga
     6781 tttcgaacat ttgccggggg atcagctcct tcatcttctc agccagctga cgcccccgcg
     6841 gatacgagcg ctctttgtgt gtaataaaag agagcgcgtc aaccgcctct ccgtttaaga
     6901 gaatgtcgag cttgaccaga tccgattctc gatagccaag caactcatag tccagcgacg
     6961 catagccttg cgtgcgagac ttcagcttgt cgtaaaaatc caagatgact tcgttcaagg
     7021 gcatttcgta gctgatcaag acgcgcgtgg gatcgagaaa atggatgctg cgttgcgtcc
     7081 cgcgccgctc ctgacacaac tgaagaatgg ctcccatgt
//
LOCUS       DASSCQ010000095         7829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1763152, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000095 DASSCQ010000000
VERSION     DASSCQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763152"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1270..1500
                     /locus_tag="VFH05_06595"
     CDS             1270..1500
                     /locus_tag="VFH05_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677915.1"
                     /translation="MASDTRMTVYLQPKVYRALKVKSATTDRSISELVNAAVIEALRE
                     DAIDVEAFDKRSKEATRSFEQVLKDLKRDGLL"
     gene            1634..1852
                     /locus_tag="VFH05_06600"
     CDS             1634..1852
                     /locus_tag="VFH05_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET6677916.1"
                     /translation="MAFDRIKVDPNVCGGKPTVRGLRITVDFVLKLLGDGYTAKQIIG
                     EYPELELDDVYQAAKYGAWLASERTTAV"
     gene            complement(2548..2751)
                     /locus_tag="VFH05_06605"
     CDS             complement(2548..2751)
                     /locus_tag="VFH05_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677917.1"
                     /translation="MKRQYRLDVSTLKLPPGAGDLRLVHGLHLLPAKGRKGVTMDFAI
                     ILIVSNTLLAGFIYFVVTRIWQD"
     gene            2968..3549
                     /locus_tag="VFH05_06610"
     CDS             2968..3549
                     /locus_tag="VFH05_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677918.1"
                     /translation="MELLLPPSCETALLERFLKAEAMALWAVRSAQAKDVPPGVLTFL
                     RRHEEEEAKHLKEFETRLGVQSHVRETLPRMPSQWWALVIQLFGYEVLGLEFAKLLVQ
                     VQPDLQSILEDEQVHVAFFEQEVKKVLAQRGTTAEGARQSARAWWRRLPSTVERYLEG
                     DSLTPFRSELKARIMASIFTRFESLTLLDGRAE"
     gene            3560..4081
                     /locus_tag="VFH05_06615"
     CDS             3560..4081
                     /locus_tag="VFH05_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6677919.1"
                     /translation="MDCILLRHGIAVERDEWEGTDADRPLTERGAKRVTQVAAGLKWL
                     GVQPTDIFSSSLVRAMETANILHAALNVPTAIHRVEELLPDAPPDHLLLLFRDLSEES
                     CVVCIGHEPHLGLAASMMLTGRPSTAFPFKKAGACLIELPTPPKIARGVLRWWMEPAQ
                     LRALGKKRAKLEV"
     gene            complement(4090..4851)
                     /locus_tag="VFH05_06620"
     CDS             complement(4090..4851)
                     /locus_tag="VFH05_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HET6677920.1"
                     /translation="MGDAHYFGDGLTYLNPSDLKGKLIAIEGTDGVGRSTHIELLQEW
                     LEVQGYGVITTGWTRSNLMSKTIEVAKEGNTIDRWSLSLLYATDFADRLEHQIIPALR
                     SGFIVLADRYIYTAFARDFVRSNDRKWIRDVFGFALVPDLVCYLRVDVETLALRVIET
                     KAMNYWESGMDLRLGNDLYDSFKKYQSLLIEEFDKMAEEFKFEVVDARKSLEEIQEEL
                     RTRIQQLLTEEGLARTTAVVSDRPDEHTQSPDLVT"
     gene            complement(4799..5551)
                     /gene="tmk"
                     /locus_tag="VFH05_06625"
     CDS             complement(4799..5551)
                     /gene="tmk"
                     /locus_tag="VFH05_06625"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET6677921.1"
                     /translation="MSEQPPNTPTPPHPYPGKLIIVEGIDGSGKSTQLLLLHKWLESK
                     GHKVFFTEWNSSELVKDTTKRGKKNKSLTPTTFSLLHATDFASRLYHEILPPLKAGML
                     VLADRYMYTAFARDVVRGVSAEWVRKLYSFAIKPDMAFYFKVPIEVALSRLLGGTRGQ
                     FKYYEAGMDMNLSQDVTESFRIFQSRILAQYDKIVDEYELITMDATKDIADQQDSMRR
                     LVGEALKDYKPRRGTHGRRSLFWRRFDLPKSE"
     gene            complement(5548..7098)
                     /locus_tag="VFH05_06630"
     CDS             complement(5548..7098)
                     /locus_tag="VFH05_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET6677922.1"
                     /translation="MSKIAVIDIGTNSIHMVLAEVLPDFSYKILDRFKDIARLGDGAF
                     ANQRLSEEAMARGLSVINRLATLAKNKGYDRLIAVATSAVREAKNGGDFIDLVKEQTG
                     LTVRVISGTEEARLIFLGIKNSVPMTEQASLAVDVGGGSVEIMAGNRDQLLHAKSLKL
                     GAIRLADQFLKRTPPSDRMLRELEDLVTGQLAGTLESFKTKRFESLIATSGMAANLAE
                     VIHLRRTNRPLPQLNLATVSLKEVKEIEQVLRRSTIKERQAIPGLDPKRVDTLFPATL
                     VLRRLMELVGRDELILCDKAIREGIIFDFIVRHRERIKAEADMPDLRRRNVLALARRC
                     QVPEVHSLHVASLALRLFDQTKRLHNLGELEREWLEFAAVLHDIGYLINERQHHKHTY
                     YLITHSELGGLSAEEIVIVANIARYHRRALPHSKHDPFDALTPKLQRTVRILSALLRI
                     ADGLDRTHFSVVRTLDVKIGATIKIIVYVTGDAELEAWAAKGRSDLFERIFRRRVQFT
                     LKPQEDAA"
     gene            7257..>7829
                     /locus_tag="VFH05_06635"
     CDS             7257..>7829
                     /locus_tag="VFH05_06635"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6677923.1"
                     /translation="MTVQGASRFIVITGASTGIGAACAMGCAQQGMTVFAGVRDLPAG
                     EALRARGCARIVPIHLDVTDTKSIHEAAQVVEERVGSQGLWGLVNNAGIAIASPLELI
                     PLAELRRQFEVNVIGQIAVTQALLPLIRRARGRVVNMGSIAGRGTIPMMGPYSASKFA
                     LEALTDALRLELYPWGIEVSIIEPGAIATPI"
BASE COUNT         1667 a         2074 c         2225 g         1863 t
ORIGIN      
        1 gccctcgaag cgtgaaaggc gacgagtcat gctcggcgaa gcgccgagat ttcaaatcct
       61 aaatgttcag atctcaaatg ttgactgaga aactcagtca gcgtgtcgac agaacccacc
      121 acttctgcag gtcacgcgtt aagcgattct gatacccata ggcctgcaag gtcagagctc
      181 ccaccaacag gatatcgaga gaagcgccgg tctccctggt gtaatctgtc agcagctgga
      241 tgacttgatc agcggtgagc gcagacaatg ggtcctgaat cgaatggcag gggagttcca
      301 gctcagaacg ctctttgaaa atccggtttg gttaagactc tgacaatgtc aatacgagcg
      361 cctgatcgag tgaatacgac tcggtagtcg cccgctctca gacggtagat gtttacttta
      421 tacccttgta atttcttgat ccgctttccg tcgggcaccg gatccttacc gagacgggta
      481 caatcagcca gaatttgttc tctgattcga ttcgggaaag cgtccaggtc acgctgagct
      541 ttctcggaaa ggaagacctc gtaggtcacg ctttacgcgc gggtgttttc ttgtgcctcg
      601 gcttgtactc agacaatcgc atcgtacgac cggcacgtac gtctgctagt ccttcttcta
      661 tcttcttacg gagacggggg ctataagcca gcagcgcatc ttcaatgtcc tcctctgtga
      721 ctggcaccaa catggcagtc ggtcgcccat gtgtggtcac aaggactgtg ttgccccgtt
      781 caacggtcct gagcatttca gacgttttac tcttgagttc tcgtacgttc gtaaatttca
      841 taagctcctc ttgtagctac atgtgtgacc acacattagt gtgcgactac aaagaagtca
      901 acagactttc tgagatctgg ggaatcgtgg aatttcagga gtgattagaa tgaaaatagg
      961 tcggcggcgt gaaaaggccg cgctgccagt cttagtggaa gcgcggccta gagcgtgaag
     1021 gcaaccggtc agcttttgga aggatcagga ccagaacctg cgtggtcgtt ggcgcttggc
     1081 agcgcgacca cagtaatatt ttagaggaag gcaagaacgt cgatgtattc cacttggctg
     1141 ctggtgtgag aaattagcag accattcgct tttacttctt ccaattgaaa ctagatggct
     1201 tcccgaataa gagactgagt ctctgtcggg tccgtgctat agtaccatct cgttttgatc
     1261 gaggatgtca tggccagtga tacccgtatg accgtgtact tgcagcccaa ggtataccgt
     1321 gcactcaaag taaagtcggc gacgaccgat cgatcaatct ccgagttagt gaatgctgcg
     1381 gtgatcgagg cactccgtga agacgctatt gatgttgaag cgttcgacaa gcggagcaag
     1441 gaagcaaccc gctctttcga acaggtcctg aaagatctca agcgtgacgg actcttatag
     1501 tctcgtcgtc aagaagtctg cagaacggga gctgcgtgca atagatctct gagagatagt
     1561 tgtttcctgg cagagagctt catctatagc cctttacgct tgcgtctctg attagaaacg
     1621 agtaccgtaa ctcatggcat ttgatcgcat caaagtcgat cccaacgtct gtggagggaa
     1681 gccgacagtg agaggcctcc gcattacggt ggattttgtt ctcaagttgc tcggcgatgg
     1741 atatacggca aaacagatta tcggggaata tcccgagctt gagcttgacg atgtgtatca
     1801 ggcggccaaa tacggcgcct ggctggccag tgagagaacg accgctgtgt aatgaggctt
     1861 cttgccgatt tgcacatctc acctcgcacc gttgaatatc tacagtctct cggtcatgac
     1921 gttgtccgtg tatctcaggt gcttcctccg acgagtcctg atcaccaaat ctcgcatatg
     1981 cagtcagtga tcgtcgtgcc attctgacgc aagatctgga cttttctgct gagattgcag
     2041 tatcgggaca agcctttcct tctgtggttc tcttgcgcct ctcatcctct cgtgttgaaa
     2101 ctgtgaatgc tgttctttcc gatgtcctgc cgacattgga acaggacgtt aaagacggcg
     2161 cgttaattac cgttgaagac caccgtctcc ggcgacggag attgcctctc tcgtctgcgt
     2221 agtctaaaaa tcttcaggaa tctgaaaata aagaaaggcc gcgctccaat gttattggag
     2281 cgcggcctaa agcgtgaagg caaccggtca gttttctgga agggtcagga ccagaacctg
     2341 cttggccatc ggttggcgct tgtcagcgac ggcccctggt cggcttcaca cctctcaatg
     2401 ttcggattgg ccgatcgctt atcgcacggt cctacgactg agttaccgcc tcaataagcc
     2461 atcggtcaaa aaacctgaca gtcggcgcta ctatcgcgtc ttctacgttg ctcaacagtc
     2521 cgcttttaga attgggcatc gattcgttta gtcttgccag atgcgagtca caacgaaata
     2581 gatgaagcct gccagcagtg tgttactcac aataagaatg atcgcgaaat ccatggtgac
     2641 tcctttccga ccctttgccg ggagtaagtg caaaccatgg accagacgga ggtcgccggc
     2701 ccccggtgga agcttcaacg ttgaaacatc aagcctgtat tgacgtttca ttcatatcac
     2761 cctcttccct acatcgcgtg gtgacaataa atggcaccac tttggtgtgc cagatttgat
     2821 tctgcctgga tcgaattgga ttcttgcaac ctgcgctatt atttgaggaa tttgcatata
     2881 tggaggcatc actgaatgtc ggtgtttgcg ggaaattcgc cgttggtttc tgaggcgaag
     2941 tttcgttaca cttgaccgat gccgcccatg gaattgctgc tgccgccctc ttgtgaaacc
     3001 gcgttgctcg aacgattcct caaggccgag gcaatggccc tctgggcggt gcgctcggct
     3061 caagcgaagg atgtgccgcc tggcgtgctc acgttcttaa gacggcatga ggaggaagag
     3121 gcgaagcatt taaaagagtt cgaaaccagg ctcggcgtgc aatcgcacgt ccgtgagact
     3181 cttccgcgca tgccgagcca gtggtgggcg ttggtcatcc agctgttcgg ctacgaggta
     3241 ctgggattgg agttcgcgaa gctactggtt caggtgcagc cggaccttca atccattctg
     3301 gaggatgagc aagtgcacgt cgcctttttc gaacaggaag tgaagaaggt gctcgctcag
     3361 cgcgggacga cggccgaggg agcaaggcag tccgcgaggg cctggtggcg ccgcttgcct
     3421 tcgacggtgg aacgctacct ggaaggtgat tccctcacgc cgtttcgttc cgagctgaag
     3481 gcacgcatta tggcaagcat ttttacccgg tttgagagcc tgacgttgtt ggacggcagg
     3541 gcagaatgag gcgagaagta tggactgcat attactaaga catggcattg cggtcgagcg
     3601 ggacgagtgg gagggtaccg atgccgaccg gccgttgact gaacgagggg ccaagcgcgt
     3661 gacccaggta gcagcaggtc tcaagtggct gggcgtgcaa ccaaccgaca tcttttccag
     3721 ttcattggtt cgtgcaatgg aaacagccaa catcctgcat gccgccttga acgtacccac
     3781 ggcgatccat cgggtggagg aactcttgcc ggatgcgccg ccggaccatc tgcttctgct
     3841 gttccgcgat ctttctgagg agtcctgcgt ggtgtgcatc gggcacgaac cccatttagg
     3901 gctggcggcg agcatgatgc tgaccggcag gccatcgacg gcttttccgt ttaagaaggc
     3961 gggtgcctgt ctcatcgagc tacccacgcc tcccaagatc gcacggggag tgctgcgttg
     4021 gtggatggag cctgctcaat tgagggcgct gggaaagaag cgggccaagc tggaagtgtg
     4081 aggcgtttgt taagtcacga ggtcgggact ctgagtgtgt tcatccgggc ggtcgctcac
     4141 gacggccgtc gtgcgggcca gaccttcttc cgtgagcaat tgttggattc ttgtgcgtaa
     4201 ctcttcttga atctcctcca gcgattttct ggcatccacg acttcgaatt tgaactcttc
     4261 cgccatcttg tcgaattctt caatcagcag cgactgatat ttcttgaagc tgtcgtagag
     4321 atcgtttccc agccgcaggt ccatgccgga ttcccaatag ttcatggctt tggtttcgat
     4381 gacgcgtaat gccagggttt cgacgtcgac acggaggtaa cagaccaggt ccgggaccag
     4441 cgcgaagcca aagacgtctc tgatccactt ccggtcgttg ctccggacaa aatccctggc
     4501 aaaggcggtg tagatatagc ggtcggcaag cacaataaat ccggaacgca gcgcagggat
     4561 aatctgatgc tccagccggt cggcgaagtc cgtggcgtag aggaggctca acgaccatcg
     4621 atcgatcgtg ttgccttcct tggcaacttc gatcgtcttc gacatgaggt tcgatctggt
     4681 ccagccggtg gtgatgactc catacccctg gacttcgagc cactcttgca acagctcgat
     4741 atgcgtggag cgaccgacgc cgtcggtgcc ttcgatcgcg atcagtttgc ccttgaggtc
     4801 actcggattt aggtaggtca aaccgtcgcc aaaatagtga gcgtctccca tgggtccccc
     4861 gtctgggttt gtagtctttc agcgcctcgc cgacgagacg gcgcatacta tcctgctgat
     4921 ccgcgatgtc tttggtcgca tccatcgtga tgagctcata ctcgtccacg atcttgtcgt
     4981 actgcgccag gatacgcgac tggaaaatgc ggaagctttc cgtgacgtcc tggctcaaat
     5041 tcatgtccat gcccgcttcg taatatttga attgcccccg cgttccgccc aacaaacgcg
     5101 agagtgccac ctcgatcgga accttaaagt agaaggccat gtcaggtttg atggcaaagc
     5161 tgtacagctt gcggacccat tcggcggaca cgccccggac cacgtcgcgc gcaaaggccg
     5221 tgtacatgta tcgatccgcc agcacgagca tgccggcctt cagcggggga agaatctcat
     5281 ggtacagcct gctggcgaag tcggtggcgt ggagcaagct gaacgttgtc ggagtcaggc
     5341 ttttgttttt cttgccgcgc ttggtcgtgt ccttgaccaa ttcagacgag ttccattcgg
     5401 tgaagaagac tttatgacct ttcgattcca gccacttgtg caaaagcagc agctgcgtgc
     5461 tcttgcccga gccatcgata ccctcgacaa tgatcaattt gccgggatag ggatggggag
     5521 gcgtgggggt gttggggggc tgttcgctca tgccgcatcc tcttgaggtt ttaacgtgaa
     5581 ttgtacccgc cggcggaaga tccgttcaaa taggtctgac cgtcctttcg cggcccaggc
     5641 ttccaattcg gcatcgcccg tcacatagac aataatctta atagttgcgc ctattttgac
     5701 gtccagggtc ctgacgactg agaagtgggt acggtccagg ccgtcagcaa tccgaagcaa
     5761 cgcggacagg atacggacgg ttcgctggag cttaggagtg agcgcatcga aggggtcatg
     5821 tttgctgtgc ggcagtgcac gccggtggta tcgcgcgata ttggccacga tgacgatttc
     5881 ctcggccgag agccctccca gttcactatg ggtgatcagg tagtacgtgt gcttgtggtg
     5941 ctgccgttca ttgatgaggt agccgatgtc atggagcact gccgcaaact cgagccactc
     6001 gcgttcgagc tcgcccaaat tatgcagccg tttggtctga tcgaagagcc gcaaggcgag
     6061 acttgcgacg tggaggctgt ggacttccgg gacctggcat cgcctcgcga gagccagcac
     6121 gttccggcgt cgcagatccg gcatgtcggc ttctgctttg attcgctccc gatgccggac
     6181 gataaaatcg aaaatgatgc cctctcgaat ggccttgtcg cagaggatca attcgtcgcg
     6241 tccgaccaat tccatcagac ggcgaagaac gagcgtggcc ggaaacagcg tgtccacgcg
     6301 tttgggatcc agaccgggaa tagcctggcg ctctttgatc gtcgaccgac gcagcacctg
     6361 ctcaatctcc ttcacttcct tcagcgagac ggtggccagg ttgagctgcg gtagagggcg
     6421 gttcgttcgc cgcagatgaa tcacctcggc gaggttggcg gccattcctg acgtcgcaat
     6481 gagactctcg aagcgtttgg tcttgaagct ctcgagcgtc ccggccaatt gtcccgtcac
     6541 caggtcttca agttcacgca acattctgtc ggacggcggc gtgcgtttca gaaattgatc
     6601 ggcgagtctg atggcaccga gtttgaggct cttggcatgc aggagttgat ctcggttgcc
     6661 cgccatgatt tccaccgaac ctccgccgac atccaccgcc agcgacgctt gctcggtcat
     6721 gggcacgcta tttttaatgc ccaggaagat caaacgcgct tcttccgttc cgctgatgac
     6781 ccggacggtg agcccggtct gctccttcac gagatcgatg aagtcgccgc cgttcttcgc
     6841 ttcgcgaacg gcgctggtgg ccacggcgat gagccgatcg taccccttgt tttttgccag
     6901 cgtcgcgagc cggttgatga cgctgaggcc cctcgccatc gcctcctctg acagacgttg
     6961 attggcgaac gcgccgtccc caagtctcgc gatatctttg aaccggtcga gaatcttgta
     7021 gctgaaatcg ggcaggacct cggcgagcac catgtggatc gagttggtac cgatatcaat
     7081 cacggcaatt tttgacatgt cggaatcggc tgattataag acccaagaca ggctgtaccg
     7141 gtcacgccat tgtagatgaa tccagagggc gtagtgaaga aatctctctg gaatacagct
     7201 cgtgattgta aactttctgt tacaatccgc gagaattgaa agaaaataaa tctgagatga
     7261 ctgtccaagg tgcttcgcga ttcatcgtga tcacgggcgc gtccaccggc attggcgcag
     7321 cctgcgcgat gggttgtgcg cagcagggca tgaccgtatt tgccggtgtc cgtgatttgc
     7381 cggcgggtga agcccttcgc gccaggggat gcgcgaggat tgttccgata caccttgatg
     7441 tgacggacac gaaatccatt catgaagccg ctcaggtcgt ggaagagcgc gtgggctccc
     7501 agggactgtg gggactggtg aacaacgccg gtattgcaat tgcgagtccg ttagaactta
     7561 ttccgttagc agaattgcgc cgtcaattcg aagtcaacgt catcggacaa attgcggtca
     7621 cccaggcgct gcttccctta atcaggcggg cacgtggacg tgtcgtcaac atgggctcga
     7681 tcgcagggcg cggcaccatt ccgatgatgg gaccctattc agcctcaaag tttgcattgg
     7741 aggcgctcac cgatgcgttg cgtctggaac tgtatccctg ggggatcgag gtatcaatca
     7801 tcgaacctgg agccatcgcc accccgatt
//
LOCUS       DASSCQ010000096        34885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_445747, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000096 DASSCQ010000000
VERSION     DASSCQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 34885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34885
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445747"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VFH05_06640"
     CDS             <1..867
                     /locus_tag="VFH05_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6677924.1"
                     /translation="VTLGVVSGIGRENVNLSRYENFIQTDASINPGNSGGPLFNLRGE
                     IIGINTAIINFAQGIGFAIPSNMAKQVLQQLLEQGRVVRGWLGVGIQPLTPELARKFG
                     VPEQEGVLVNEVFEKDPAAAAGIKAGDVITRIEGSVIDSPNRLSRIVAGLLPGAKAHV
                     EVVRNQERLVVEVALTERRDHATVASLPQSRSESKLGVDVQDLTAALAEKFKLRESHG
                     VLVTKVEPGSLAQSEGLREGDLIKEVNRADVGTVGDFTRAVAKVRRGDTVLLRVLRES
                     RAFYVVLKPNEP"
     gene            complement(930..3032)
                     /gene="fusA"
                     /locus_tag="VFH05_06645"
     CDS             complement(930..3032)
                     /gene="fusA"
                     /locus_tag="VFH05_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET6677925.1"
                     /translation="MNEPGARSLRNVAIIANRGAGKTSLAEAILLTTGAIPSLGSVTQ
                     GTTVSDFEPEEIQHRSSVSTSLLRCTWNQTTINLLDTPGALSLLGEAITALHAADAVI
                     VVITPSSGIRSELLRLWSYVKALHLPCLIVVNEFEKESASVDDVIAVCHRELEITPLP
                     MSCLWKDGAQCQGVIDFMGQQAIQSRPDSMKWQQQPIPSGADTIIGEARKRIQEAAAE
                     CDDQLLEQYLSAGELTPADMLRGLRAGVLAGTVVPLYVGSVLKNIGTVPLLNGVVDLL
                     PSPADRAERQPLVGEHPDSHHDVSRPASPDGPFSAVIFKTIIDPFVGRLSYVRLYSGS
                     LHADSVLFNSTRRIKEKSGHLYQLLGKKHTAVSSASAGDIIAIGKLKDAQTGDTLCEE
                     AQPISYPWPTLPRPVLSFAIEPKSSADVDKVSLGLHKLIEEDPTLGFSRNADTKEMVL
                     SGTGQLHLDLALEKLQRKYGAEVVIHTPKIPYRETIRATAQAQGKYKKQTGGHGQYGD
                     CWLEVTPSARGEGFAFTNRVVGGVIPRNFIPAVEKGVVEAMQHGTLAGFPVVDVRVAV
                     YDGSYHVVDSSEMSFKIAASMAFKKAMETAHPVLLEPMMTVEVEAPAAHIGAVIGDLN
                     ARRGRIVHVEARGHAEVVKALVPLSEIMTYTTTLNSLTGGGGSYVMELARYDEVPRDV
                     AAKIVEEVKATRQAVGAH"
     gene            complement(3085..3828)
                     /gene="radC"
                     /locus_tag="VFH05_06650"
     CDS             complement(3085..3828)
                     /gene="radC"
                     /locus_tag="VFH05_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET6677926.1"
                     /translation="MEQKAARQGIAYWPVTERPRERLLTQGAEALTDAQLLAILLRVG
                     RARFSAVQVSMEVLDCLGGVSGLARCGIEELCAVPGVGEAKAAQIKAAIELGKRSLAS
                     PLSTGMKIGSSRELFAHYHPSLRDLRHEIFKVVLLDAKHAIIRDMTVSEGSLTLSIVH
                     PREVFTMAVKESAAAVIFLHNHPSGDPSPSQEDHVLTARLVSAGELLGIAVLDHIVVG
                     DGRYVSFADQGWLHHHAGRHEQREGRTTM"
     gene            4011..4208
                     /locus_tag="VFH05_06655"
     CDS             4011..4208
                     /locus_tag="VFH05_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677927.1"
                     /translation="MECPKCKGLMMLERFSDFFLIFYAWKCINCGAIIDRTISNNRRK
                     SLAARDAQAAQPAQPSVAATR"
     gene            4758..5327
                     /gene="rsmD"
                     /locus_tag="VFH05_06660"
     CDS             4758..5327
                     /gene="rsmD"
                     /locus_tag="VFH05_06660"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET6677928.1"
                     /translation="MRVIAGSHRGRRLNAPQGNSLRPTSDKVREALFSILGPRVSGSR
                     FLDLYAGTGAVGIEALSRGALVVTFVESDPKAMQAMHHNLTTCGLLEQAELRRESTDM
                     FLRQRRWQHAPYEIVFADPPYADPEAVELVLTGWTIGLLAPDAVMIIEQDARAELPVA
                     TNDARFTRRYRYGDTALFLYGAAPRGLPS"
     gene            5324..5812
                     /gene="coaD"
                     /locus_tag="VFH05_06665"
     CDS             5324..5812
                     /gene="coaD"
                     /locus_tag="VFH05_06665"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET6677929.1"
                     /translation="MKIGIYPGTFDPITHGHTDIITRSLRVFDKVMVAVAPNPTKHPL
                     FNLAERLEMVQLVLKDIGQVEVVTFDGLLVDHVQRSGAHAIIRGLRAISDFEHEFQMA
                     LINRKLAKQVETVFLMPSEEYSYLSSTIIKDVANHGGTLTEFLHPEVARRLQARIRSV
                     KS"
     gene            5809..7002
                     /locus_tag="VFH05_06670"
     CDS             5809..7002
                     /locus_tag="VFH05_06670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6677930.1"
                     /translation="MKLAARVSRIAPSPTLAMAATAKAMAAQGIDVIDFSTGEPDFDT
                     PEPVKAAAEAAIRSGFTKYTPSSGIDELRSAIAEKLKIEQGLQYDKSQILVSCGAKHS
                     LYNVAEALLEAGDEIIIPTPYWVSYADQAFLNDATPVLFATREADGYAIDSAALEQRI
                     TPRTKALIINSPSNPTGATYNRPMLERIAAIAVRHDLLVISDEIYEKVLYDGATHVSI
                     ASLGPDIAARTVVINGVSKAYAMTGWRIGYAAGPKELITAMGNIQSQSTSNPCSISQK
                     AAVAALRFGGPFTKQMVTEFDRRRRAIVEGLNTIPGISCSMPAGAFYAFPNIQGVLGK
                     RGPTGPITSPTDLATYLLKDAHIAVVPGEPFGSQSHMRLSYATSMETIGRGLDRLRAA
                     LQKLG"
     gene            7058..7420
                     /locus_tag="VFH05_06675"
     CDS             7058..7420
                     /locus_tag="VFH05_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET6677931.1"
                     /translation="MPIYEYQCDSCSYQFEVKQSMKDAPLTTCERCGKSLRRLISSPG
                     IMFKGSGWYVTDYSNKLKPPSDGEKPATGEKKETPAGSPAPASTAAAPTGTSSTPAST
                     SSTSSSATTPPTTNKPKS"
     gene            complement(7439..7864)
                     /locus_tag="VFH05_06680"
     CDS             complement(7439..7864)
                     /locus_tag="VFH05_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677932.1"
                     /translation="MPIRDTCRPRRAAGLVMTAAVLLAGCDYWPPALQTQIEQLRSEL
                     QTVTMEKAQLQNQMTSLTKAKDDLQMQVDDLSRTNRDKSTLIASLQSSVSALQERLAK
                     SSKPVAAKAPLAKVTPKPAVKTAQKAPVKKKTLTKSAVH"
     gene            complement(7910..8560)
                     /locus_tag="VFH05_06685"
     CDS             complement(7910..8560)
                     /locus_tag="VFH05_06685"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6677933.1"
                     /translation="MAPIESYSPSTVERLSRQVASVLQRSGLVAIPTETFYGLGVNPF
                     DARALDRLRAVKGREDAKPILVLVASINDLSAFVEQVPPAASLLIEGFWPGPLTILFP
                     ARASLPRALTAGTGLVGMRLSSCRPLCELLARVGPLTGTSANRAGAAPARTAEAVQQA
                     LGDAVDLIFDAGPTPGGLPSTVVDAHHSLRIVREGAIERSVIEARLHQRGFSLKNS"
     gene            complement(8650..9921)
                     /gene="purD"
                     /locus_tag="VFH05_06690"
     CDS             complement(8650..9921)
                     /gene="purD"
                     /locus_tag="VFH05_06690"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET6677934.1"
                     /translation="MKVLVVGNGGREHALVWKLVQSPRRPQVFCAPGNAGIETLAACV
                     PVNANDLAALKTFAQREGIELTVVGPEAPLALGIVDEFRKAKLKIFGPTKQAARLEAS
                     KIFSKAIMAQAHIRTARSQAFDKPADALAYLEDHELPVVIKADGLAQGKGVLIALTRE
                     EARHAIVDSMEHAVFGEAGKQVLIEEFLEGEELTIMAFTDGRSVAPMLPAQDHKRVGD
                     ADTGLNTGGMGAYCPAPLGTPALRAQVTKEILQPIVDAMSRLGSPFQGVLYAGLMVVG
                     GVPYVLEFNARLGDPETQVVLPLLKTDLLDVMEAVVEHRLDQLRVEWHDEHAVCVVMT
                     SAGYPGPYQSGMRITGLDAPFEDAAVVFHAGTAKRNNDIVTAGGRVLGVTGRGGTLAE
                     AQAVAYRTAERIAFTGCHYRRDIAHRALVTR"
     gene            complement(10043..11596)
                     /gene="purH"
                     /locus_tag="VFH05_06695"
     CDS             complement(10043..11596)
                     /gene="purH"
                     /locus_tag="VFH05_06695"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET6677935.1"
                     /translation="MASMKRALISVSDKTGVVDMAQGLTALGAEILSTGGTAKALRDA
                     GIMVTEVAAYTGSPEILDGRVKTLHPKIHGGLLGRRSIPAHVEQMKQHNIGPIDVVVV
                     NLYPFEATIAKPNCHFDEAIENIDIGGPSMLRSAAKNHEDVLVVVDPADYQRVLEALK
                     DNTVTRALRRELAMKVFQHTARYDSLIAGYMEKQVQSGAAKFPAILSLQYELGEILRY
                     GENPHQQGAFYRERQSTEPSVSHGKILHGKAMSYNNYLDANAALELAKEYDEIAVAII
                     KHNNPCGVALGATSVEAYVKARETDPTSAFGGVIAFNRAVDLPAAKEITSTFVEVVVA
                     PGFSEEALAELRRKKDLRLLDVGALTKVKQEGYDFKKLVGGLIVQDRDLGTLPDVRML
                     SVPTVRKPTDEEYTACAFAWKVCKHVKSNAIIYAKPGQTVGIGAGQMSRVDSVKLAAM
                     KAQLPMKGCVMASDAFFPFRDGLDAAAQAGITAVIQPGGSIRDAEVIKAADEHGLAMI
                     LTGMRHFRH"
     gene            11746..12627
                     /locus_tag="VFH05_06700"
     CDS             11746..12627
                     /locus_tag="VFH05_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6677936.1"
                     /translation="MLAVDWLKRMERRTILGGGFFALTLLALVCIPRHLPSSASAPPP
                     VPANFHAHLEHGALLLRGSLPNAGAHEGILRQARAAYDPTQVRIVDQLTVDSRIASPP
                     WVNQLPAILPVLEPMQGARSVMIDGRFVVLSGAVPSQQIKAKILNTVSPLRGLGLELE
                     DHMTIAGPAASASLQARVDELLSHSQIEFESNQATLTARGQAVLDRLIPVLKEAPRTS
                     IEIGGHTDGFGAADYNKELSRRRAEAVRHYLMAHGVTHELSAVGYGASKPLAAGMTRA
                     ELQRNRRIELRVLETNG"
     gene            12627..13802
                     /locus_tag="VFH05_06705"
     CDS             12627..13802
                     /locus_tag="VFH05_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677937.1"
                     /translation="MIGLITQILGCLLVAAAIGFAVGWLLRNRSAESDEHQLTDRETE
                     LRIKGQALDTAIYELKVKTSALSALESKVSSLESLGRSAQHELASRQERIDRLQQELK
                     ESQARVASLETEQQARRERLAEQDTMLSAYANEARQANAARTESQEQLALKEQELLEL
                     QQRLVESDNRLAEIDRLRSQLAELEPAQGRVHWLEVQLSEKDAQHRRALHALEDDLSA
                     RDRRLAELAQQAQLLHDRDRQIAGLELRVAQLQALQAEMAGQAKTMGEQEAEISRLRK
                     RLGEFRAALRGRDDGSPVLVRPNGPANQLTLQIPSPKASTSPRKDDLKKIPGIGPAIE
                     RALNKMGTFTYVQIARWKDTDIARVARKLDTVPGRIKRDNWIAGAKRQHREKYGEKL"
     gene            13868..17329
                     /gene="dnaE"
                     /locus_tag="VFH05_06710"
     CDS             13868..17329
                     /gene="dnaE"
                     /locus_tag="VFH05_06710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6677938.1"
                     /translation="MSASFVHLHLHTQFSLLDGANQIEPLIQRVKAFGQPAVAMTDHG
                     NMFGAIEFYRKAKEHGIKPIIGCEAYMALGSRHAKKDSGLAHNDYYHLILLARNVTGY
                     QNLIKLVSKAYLEGFYYKPRMDKELLKEHHEGLIALSGCLSGEIPYLIGQKDMKAALA
                     VAQEFQEIFGRDQFYLEVQANGLDHQRIANAGLIEIHKQLGIPLAGTNDCHYLKKEDA
                     RPHELMLCLQTGKTLSDPTRMKFDTDQLYVKSTEEIAPAFAEFPGAVANTCRIADDCE
                     LELALNTTHLPQYKAPEGYTRESYVEHLAVEGLKSRLSERPSRIALAVYEQRLREELM
                     VICSMGFAGYFLIVWDIIRFARSRGIPVGPGRGSAAGSLVAYALRITDLDPLVYTLLF
                     ERFLNPERVSLPDIDMDFCMDRRGEVLNYVVDKYGSDHVAQIITFGTLGAKAAIRDVG
                     RVLEMPYAEADKVAKLVPNQLNITLEQALESEPRLRELVDTDPKVRELMSIARSLEGL
                     ARHASTHAAGVVISEGPLTDHVPLYKGANDEIVTQYSMGDVEKIGLVKFDFLGLKTLT
                     MIRRAESLINESRPDQTPLVVDRLPFDDAKTFALLSSGKTTGIFQLESSGMRDLLTGF
                     KPDRFEDIIAIIALYRPGPMDLIPDFIKRKQGKIPIVYELPELEPILKDTYGVIVYQE
                     QVMAIANRVAGFSLGQADILRRAMGKKKPEEMEKLRVKFVDGARQNKIADRKAEKLYE
                     LIQKFAGYGFNKSHAAAYAVVCYQTGYLKAHYPTEFMAALMTSDMGNQDKIMGYFSEC
                     RDLGIKVLGPDVNESQKNFAVVDGAIRFGLAAIKNVGEGAVDSVLAIRTEGGRFTSFF
                     EFCRRVDLHKVNKRMLEGLIKAGAFDSFGARRAQLMAMLDQAVEDGAAAQRERDLGQT
                     NIFGEDVHGGEHSTAAVEPALPNLPEWDQAQRLKYERELTGFYITAHPLARYEATMHA
                     LATATTAGMTEFSDGKEVRLCGIIAAVKSMLTKKGDRMAYVTLEDLQGTVEIIVFPDL
                     FKTAGELITPERLVRITGTIDRGDKGTKVRGTKIEPLADVQTQTIKRIHIRLAACPEA
                     KDQLPRLLDVFKRHPGGTAISFTFRTDGLLEAETAPLPNLRVNASEHFVADVEEVLGK
                     GALSLVS"
     gene            17391..18359
                     /locus_tag="VFH05_06715"
     CDS             17391..18359
                     /locus_tag="VFH05_06715"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET6677939.1"
                     /translation="MRDYLEFEKPLREVEEKIEKLAASGGGKSGVQDEIRKLRTKLAQ
                     LEHELYSKLSPWQRTQLARHPQRPSTLDYIGEMFRDFLEFHGDRSFGDDRAIVGGFAS
                     FNGRSVMVIGHQKGKTLKERMQRNFGMPNPEGYRKALRLMKMAEKFGRPIITFIDTPG
                     AYPGIGAEERGQAEAIARNLYAMSRLSVPIVSIVIGEGGSGGALALGVSDRILMLEHG
                     VYSVISPEGCAAILWDDPAKVPDAAAALKMTAQDLVDLEIVDEVIPEPLGGAHREPRA
                     VTDRVAKALTNQLYQLTDLSVADLLDQRDRKYRRIGAVTGLDPSVN"
     gene            18557..18883
                     /locus_tag="VFH05_06720"
     CDS             18557..18883
                     /locus_tag="VFH05_06720"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6677940.1"
                     /translation="MEARKFQRFSDHSHVAFSSETVKGEGRLENLSLGGAAILSDLTI
                     ARGEYFALRITFPTQATAIEIELAPVRWVKPGSFGVEFIRIAPSSQQHLKQYLLTLEN
                     ASSDAA"
     gene            complement(18913..19494)
                     /locus_tag="VFH05_06725"
     CDS             complement(18913..19494)
                     /locus_tag="VFH05_06725"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET6677941.1"
                     /translation="MPRCVWAGSQPHMRRYHDREWGKPVHDDRRLFEMLLLEGAQAGL
                     SWETVLRRREGYRRAFAGFDPVKVATFGRDKQAALLRDVGIIRNRLKIEAAVSNARAF
                     LAVQNEFGSFDRYVWQFVEGAPIVNGWKRSSDVPATSPESDRLSKDLKKRGFRFVGST
                     IMYAFMQAVGMVNDHTLDCFLAGKRPRRRPLRL"
     gene            19828..21369
                     /locus_tag="VFH05_06730"
     CDS             19828..21369
                     /locus_tag="VFH05_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HET6677942.1"
                     /translation="MKYQLIPACLVMLGLTACSHTIPETYTKPQAPPQLGKTPPVIQP
                     LNATQKQAQAPESLLPVTMTADLTPVQKTIQAALPERFTEEGHPLAKDYRWRFIREGE
                     PQVQIQDGLVTFSATYRGEIESAAARACRLDPLYPVLEGTGRLELREQDQGLLVTMSQ
                     PQTSMNLKPESDTKCNMFNLPVKDQLAELFQQESVRQHIVQSLEQAGYVIPLNLIWDR
                     LQEPMPVSSGNTKLCLYGKAKDFAIGALKGPVQNTTITGVVRQTPVALFQTPCQKPGS
                     TPLKMHMDSAATAMQEGQPYRILLSVPVPYAVLNQQLQERLYHQGVKLPTTFDNDLTI
                     ERVTASDVTGRTLFAVETSGDTKGTLYYWGTPQLEQEGNMITIPDLQIANETKIALEE
                     IKSGYWQMVDDQLKPRLRQASTLDLSQRIGTMRSALSGQHKSGSVAMDLLVAKQEAGQ
                     VVSTKDALVADVFLEGTASAAGRLPVKQASRGDIDQNRPVATDRTPAAEAAPKAARNP
                     DEERR"
     gene            complement(21465..22436)
                     /locus_tag="VFH05_06735"
     CDS             complement(21465..22436)
                     /locus_tag="VFH05_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6677943.1"
                     /translation="MHYVHLGRSGVKVSRLCLGTMNFGPETTEADSVAIMDRALELGI
                     NFFDTANVYGWKVGEGWTEQIIGRWFAQGGGRREKVVLATKLYGRMGDWPNQARLSAL
                     HIKRACEGSLRRLQTDCIDLYQMHHVDRNTPWDEIWQAMEQLVREGKILYVGTSNFAG
                     WQLAQAQELARSRHFLGIVSEQSLYNLNERTIELEVLPACAAYGIGMIPWSPLARGLL
                     AGALTPSTVGRRADKEVQQEVEKYRPRLEAYEKLCHELGERPADVAIAWLLHQPAVTS
                     PIIGPRTLDQLVSTMRVMNLTLATETLKRLDGIFPGPGGPAPEAYAW"
     gene            complement(22490..23329)
                     /locus_tag="VFH05_06740"
     CDS             complement(22490..23329)
                     /locus_tag="VFH05_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6677944.1"
                     /translation="MTLSAYNGVTMPAFMYGTAWKKEATTQLVIEAITAGFSAIDTAN
                     QLIHYDEARVGEALVSLAQGGHTRDRLFLQTKFTPVNGQDHRTPYDARAPITTQVRQS
                     FESSLTHLRTDYLDSYVLHGPYSRSGLGPEDWEVWSAIESLYASGKSRLIGVSNVTAG
                     QLALLCERATHRPMVVQNRCYAAFGWDKAVRDLCRAQHIVYQGFSLLTANREVFAEPG
                     IRAMAEKYGTGVAQIVFRFAMQVGMLPLTGTTNREHMTADLQCDRFTLTPDELERIET
                     IGL"
     gene            23448..23771
                     /locus_tag="VFH05_06745"
     CDS             23448..23771
                     /locus_tag="VFH05_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677945.1"
                     /translation="MNPESEIVELRFSYRYMKQHAWVVQAVNGFLSAYFMEQPGFRVQ
                     RHFDELESGMHVWLCEVPAAMKMTRLLKRLKADIPPFRQTETRSSADSRAQYVIDSPD
                     PDSPA"
     gene            23852..24940
                     /locus_tag="VFH05_06750"
     CDS             23852..24940
                     /locus_tag="VFH05_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET6677946.1"
                     /translation="MHRVLVLGAGKIGSLVACLLSQQGAYEVHLADLTLDAPKHLVED
                     LGLSRITPAVLDVRHPETVETYLSAHPVDAIISSLPYFCNPTVAALALKHRCHYFDLT
                     EDVEVTNHIRALSAGAAHAFMPQCGLAPGFISIVTHELMTHFETLDHVKMRVGALPVH
                     PSNALKYSLTWSTDGLINEYGNLCYGIEDGRKIPLQPLEGYETIELDGLLYEAFNTSG
                     GLGTLADSSVGSVKTMNYKTLRYPGHCEKIQLLMKDLKLNEDRKTLKRVLEHAIPQTL
                     QDVVLIYASVTGTKHGEFFEENYVKKIYPQCIKGKLWSAIQVTTASSVCCVMDIVLRN
                     GAAYRGFVTQESIGLQDFLANDFGACFR"
     gene            24937..26463
                     /locus_tag="VFH05_06755"
     CDS             24937..26463
                     /locus_tag="VFH05_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6677947.1"
                     /translation="MSTVHAALKDLGIQAVNSGGSTGKHWWSDHATGSLIQSVNPATG
                     EPIAAVRSCSAGDYEHIATQARDVFATWRMVPAPKRGEVVRLIGHALRSKKDQLGTLV
                     SLEVGKIKAEGDGEVQEMIDMADFAVGQSRMLYGATMQSERPSHRMSEQWHPLGPVGV
                     ITAFNFPVAVWAWNAFLAAIAGDPVIWKPSPKAPLCAIATQQICNQVMEQQGYSGIFS
                     LFITDQASLAETMVRDPRLPLISFTGSVPVGRHVASIVGQRLGRTLLELSGNNAVIVD
                     QSADLDLAVRAIVFGAVGTAGQRCTTTRRLLVHESCADTLLDRLVNAYKQVRIGNPLD
                     SDVLLGPLIDHVAVEAYRAAIDEIKKEGGEILYGGRVLTRPGYFVEPTIVRAQPHWPL
                     LQRETFAPILYAMTFRTLEEAIAIQNGVPQGLSSAMFTNNLLHSERFLSASGSDCGIA
                     NINLGTSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQTNTVNWGTDLPLAQGIKFG
                     "
     gene            26492..27088
                     /locus_tag="VFH05_06760"
     CDS             26492..27088
                     /locus_tag="VFH05_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6677948.1"
                     /translation="METNRELDGLLERMVVEYVARNRAAKILKGLLDEAGVGFYPVVD
                     HVTLRTMDIDRRAEPFLALGYAYDETLNYDDWYAKVYRKAGYPALFVDQAYADARGKT
                     SIIPGWVAAFGDQIFHHIAVRVEDIDKAVNRLKTNGVVFAGQIVGPAGGQLRQIFSAP
                     EVVDGQPFTVLELAERHRGYLGFLPPQADSLMKSTAPQ"
     gene            complement(27091..28236)
                     /locus_tag="VFH05_06765"
     CDS             complement(27091..28236)
                     /locus_tag="VFH05_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6677949.1"
                     /translation="MGIRILVADDDPDIVMSLSERLRWLGHDVVTAADGQAALAAVES
                     HALDLAFFDVSMPRLNGIEALKIVRKRWPDLPVVVVTAYGTIRLAVEAMKEGAVDFIT
                     KPFEQGQIDQTVTTLIARMGQRVEITRLMGEITHDVKNLLMPLVAGTDLLAEEIDDVF
                     KHLPTPAPAQAEQSHLACDEVISMFRNTSRRIQDRMKAIADYAALTRVSQKFESCVIG
                     KIADSVVKSLRAPAERKQVVLKTDGLHALPAIVGDEARLYSLLYNLVNNAIPEVPPHG
                     SVTIKGASAAGDDCVTLWVSDTGVGMPADLRDNLFTYRVVSTKAGGTGLGTKIIKEVV
                     DMHGGQISVESEPGQGTTFQIRLPIEGPTFLQNSDAAAQAGHSREAP"
     gene            complement(28382..29989)
                     /locus_tag="VFH05_06770"
     CDS             complement(28382..29989)
                     /locus_tag="VFH05_06770"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET6677950.1"
                     /translation="MMRTDTTEHNELAIATAKRRTFAIISHPDAGKTTLTEKLLLYSG
                     LIRTAGMVRGRKGGKTAASDWMGMEQERGISITASAMQFPYKEAIINLLDTPGHQDFS
                     EDTYRTLTAADSAIMVIDAAKGVEAQTRKLFAVCRLRRIPVLTLVNKMDLPGRPPLDL
                     MTEVEQALTIHASAVNWPIGSGSDFAGIVTRADNRVALFSKTAHGGATKVDVQTMALE
                     DLAKSGRVSPEVMTQVQHDLELLTIAGNPLSHEQFLGGEVTPVFFASALTNFGVESFL
                     DAFVQLAPAPGARLADTDDGGEQSIDPIEQPFSAYVFKLQANMNPKHRDSTAFLRVCS
                     GRFERDMVVKHHRLGRDVRLSRPHSLVAQERSTVEEAFPGDIIGIINPGVFAIGDTIS
                     VTGGFRFKPLPQFQPEIFARIRPTDVGKRKSFDKGMEQMAQEGTVQIMRSLNDLESLV
                     AAVGRLQFDVLQYRLRHEYRVETVLDTLPFTCSAWLEGDPETFKRPSASMIVKDQRGR
                     VVVLFGDHLMKTIARDRNPDHVLKDMG"
     gene            30184..31728
                     /gene="hpnR"
                     /locus_tag="VFH05_06775"
     CDS             30184..31728
                     /gene="hpnR"
                     /locus_tag="VFH05_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HET6677951.1"
                     /translation="MNVLLVHPGPLMYTKIYLRLEPLGLELVASAARALGHRVTLLDL
                     QVESSTDYVRLLREHRPDAVGFSCNYLANVPEVVDLAKLTKRVVPRCAVFVGGHSASF
                     VAETILVHGDGAIDCILKGEGETAVGPVLAALADRDNQPGVIPGAITSAGEGPPVGML
                     EHLDAHRPARDLLRHRRKYFIGVLDPCASIEFTRGCPWDCSFCSAWTFYGRSYRVRTP
                     EAIVEDLKTVGEPGVFIVDDVAFIQAKHGLAIAEAIARQKIKKRYYLETRGDVLLRNK
                     DVFRMWKTLGLAYMFIGIEAIDEEGLALHRKRITLSKNFEALEFARSLGITVAINLIA
                     DPSWDRRRFEIVRQWCLEIPEIVNISVNTPYPGTETWHTEARHVTTRDYRLYDIQHAV
                     LPTHLPLPEFYHELVQTQQILNRKHLGWNALKSTARIAAGHLLRGQTNFVKMLWKFNS
                     VYDPRLQLADHARPVLYEMPLPRRQTSGVRTDLLYIHHARGRAGRVLDDATEQFVETT
                     RMGKTA"
     gene            complement(31767..32162)
                     /locus_tag="VFH05_06780"
     CDS             complement(31767..32162)
                     /locus_tag="VFH05_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677952.1"
                     /translation="MFGPITVPFGAKMLFNTVTLKPGITFEDIELAVGELCSVVKDTY
                     GGDKGGFLAGQVFKFSGFVSEEGSLSSSKTAEDHYVIVTYWKSFEDHERSHANELFTK
                     KFSALASMCAETKELGYDLLWQGQAGQRS"
     gene            complement(32191..33801)
                     /locus_tag="VFH05_06785"
     CDS             complement(32191..33801)
                     /locus_tag="VFH05_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET6677953.1"
                     /translation="MEGLHQLEIVILLLGIVLALTTIAQKIVVVPYPILLVIGGLILS
                     LIPGVPTVTLNPDLVFLVFLPPILWAAAYFTSWREFRRNLRPISLLAIGLVVVTSGAV
                     AVVARWLLPGMGWAEAIALGAIVSPPDAVSATAIGKRLRIPRRVVTILEGESLVNDAT
                     ALVLYRMAVGVAVGGSFVPGQGLVTFMFAATVGVAIGIAVSIATRWALCATHDSFTEI
                     AITLLAPYVAWVVGEQVHASAVLACVAGGLHLRQHFSAAVSPITRIQGRAVWDVLVFV
                     LNGFIFILIGLELRALSQAIPPGGLGPVLVTGIVVSLTAIVVRLGWVPLAALIPRWLS
                     ASLRARDPLPAWPNIFLIGWTGMRGIVTLAAALALPVATAAGTPLPFRAEIILISFTV
                     ILVTLVLQGLSLTPLIRALHLEEDRGFEQEEMLAREHAASMALTRLDTLAGESWSVGE
                     QVERLRDHYGRRMQRFGGNGHVDPECSLESAEAFRRLRHETLTAERMALIGMRNDGTI
                     SDEVLHRLEHELDIEAVRLGIGERRVHA"
     gene            complement(33958..34263)
                     /locus_tag="VFH05_06790"
     CDS             complement(33958..34263)
                     /locus_tag="VFH05_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677954.1"
                     /translation="MAFLLLLWLLCPGTVDSAFAEPAQATAAREVKGDVLYWEGEELV
                     VKDISGREVRIRVTADTKIQGASARLKTGDKILAQVPPEGPAISITLQIPDSGPPVR"
     gene            34395..34763
                     /locus_tag="VFH05_06795"
     CDS             34395..34763
                     /locus_tag="VFH05_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677955.1"
                     /translation="MAQKERSFSMQSTVQCGITCWLVLSVAVLPAAAQSDRFGGPVEF
                     RVPDRPISVPTLRFPEAAGLSSDKPSSTSGMSAPENVLTHLDKELGRTLSRSEPGRRG
                     PPATTDDQREERALAKGSFK"
BASE COUNT         7326 a        10682 c         9987 g         6890 t
ORIGIN      
        1 gtcacgttgg gagtggtcag cggcatcggg cgggaaaatg tgaatctttc gcgctacgaa
       61 aacttcatcc aaaccgacgc ctcgatcaat cccggcaatt ccggtggccc cctcttcaac
      121 cttcgcggcg agatcatcgg catcaacacc gccatcatca actttgccca aggcatcggc
      181 tttgccattc cctctaacat ggccaagcag gtcctccaac agttactcga gcaaggccgc
      241 gtggtccgcg gctggctcgg ggtcggcatt cagcccttga cgccggagtt ggccagaaaa
      301 ttcggggtgc cggaacagga aggtgtgctc gtcaacgaag tgttcgagaa ggatccggcc
      361 gccgcagccg gcatcaaagc cggagatgtc attactcgca tcgaaggctc agtgatcgac
      421 agtccgaacc gtctctcacg gatcgttgcc ggtctgctgc cgggtgccaa ggcccacgtg
      481 gaagtcgtgc ggaatcaaga acgcctcgtg gtggaagtgg ccttgaccga acgccgagac
      541 cacgccacgg tcgcctcctt accccaatcc cgcagcgaat cgaaactggg cgtcgatgtg
      601 caagacttga ccgctgcgct tgccgaaaaa ttcaaacttc gcgaaagtca cggggtgctc
      661 gtgaccaaag tcgagccagg tagcctcgcg caatccgaag gcctccgcga aggcgatctc
      721 atcaaagaag tcaaccgggc agacgttgga accgtcggcg actttacccg cgccgtcgcg
      781 aaggtccggc gtggtgacac cgtcctcttg cgtgtcttgc gtgaaagccg tgcgttttat
      841 gtcgtgttaa agccgaacga gccgtgagcc ttcgcaccac ctcgtgacgc ggtcttcgag
      901 gcctgcgatg ccaacgtctt cacgattcgc tagtgcgccc ccacggcctg cctcgtggct
      961 ttcacctctt cgacaatctt cgccgccacg tctctcggca cttcgtcgta gcgggccaac
     1021 tccatgacat agctccctcc gccaccggtc agagagttga gcgtcgtcgt gtaggtcatg
     1081 atctcgctca gggggaccag tgccttgacg acctccgcgt gtccccgcgc ttccacatgc
     1141 acaatgcgcc ctcgccgagc attcagatcg ccgatcacgg ccccgatgtg agccgcgggc
     1201 gcctcgacct ccaccgtcat catcggttcc aacagcaccg ggtgcgccgt ttccatggct
     1261 tttttgaagg ccatcgacgc cgcaatctta aaggacattt ccgatgagtc cacgacgtga
     1321 taggacccgt catacacggc cacacggacg tccacgacgg gaaaaccggc cagcgtcccg
     1381 tgttgcatcg cctccaccac acccttctcg acggccggaa tgaaattgcg agggatgacg
     1441 ccgccgacca cccggttcgt gaacgcaaat ccctccccgc gtgccgaagg agtgacttcc
     1501 agccaacagt ctccatactg cccgtgccca ccggtttgct tcttatattt gccctgcgcc
     1561 tgtgcagtcg cgcggatcgt ttccctgtac ggaattttgg gcgtgtggat gacgacttcg
     1621 gccccgtact tccgctgaag cttttccaac gccaggtcca gatgcaactg accggtgccg
     1681 ctcaatacca tctctttcgt atcggcattg cgggaaaagc ccaacgtcgg atcctcctcg
     1741 atcagtttat gcaaaccgag gctcacctta tccacatctg cacttgactt gggttcaatg
     1801 gcaaacgaca ggaccggccg aggcagcgtc ggccaaggat agctgatcgg ctgcgcctcc
     1861 tcgcataacg tatcgccggt ttgcgcatct ttgagtttgc cgatcgcgat gatgtccccg
     1921 gctgacgcgg aactcacagc cgtatgcttc ttgccaagca attgatacag atgtccgctt
     1981 ttctctttga tcctgcgggt ggaattaaag agcacggaat cggcgtgcag cgagccggag
     2041 tacaggcgga cataggacag ccgcccgacg aatggatcaa tgatcgtctt aaagatgacg
     2101 gcggaaaacg gcccatcggg gctggcaggg cgcgagacgt cgtgatgact atcaggatgc
     2161 tctccgacga gcggctgccg ttcggcgcga tcggcgggag atgggagcaa gtcgacgaca
     2221 ccattgagca gcggcacggt gccgatattc ttgagcaccg atcccacgta cagcggcacg
     2281 acggtccccg ccagcacgcc cgcccgaagc ccgcgcagca tgtccgccgg agtgagctcc
     2341 ccggcggata aatattgttc cagcagttga tcatcacatt cggcagcggc ttcctggatc
     2401 cgctttctcg cttccccgat gatggtgtcc gcgccggagg ggatcggttg ctgctgccat
     2461 ttcatgctat ccggccgcga ctggatggcc tgctgtccca tgaagtcgat gacaccctgg
     2521 cactgcgcgc catccttcca caagcaggac attggaaggg gcgtgatctc caactcgcga
     2581 tgacagaccg cgatgacgtc gtcgacggac gcggactcct tctcgaattc attgacgacg
     2641 atgagacagg gcagatggag cgctttgacg tagctccaca gccgaaggag ttcgctgcgg
     2701 atgccgctgg acggcgtgat caccacaatg acggcatcag cggcatgcag tgcggtgatg
     2761 gcttctccca gcaaactgag ggcgccgggt gtatccagga ggttgatcgt cgtctgattc
     2821 caggtgcaac gaaggaggct ggtgctgacg ctactacggt gctgtatttc ttcaggttcg
     2881 aagtccgaca ccgtcgtacc ctgcgtcacg gagcccagac tcggaatggc gccggtcgtc
     2941 aagaggatcg cttcagccag ggacgtcttt cccgcccctc gattggcgat gatggccaca
     3001 tttctgagag acctcgcacc aggctcgttc atagaaccct ccttccttcg tgcggaggcg
     3061 cgtgcctgtc cgcatgcatg caggttacat ggtcgttcgc ccctctcgtt gctcatggcg
     3121 tcctgcgtgg tggtgcagcc agccctgatc ggcaaaactg acgtaccgcc cgtcgcccac
     3181 gacgatatga tccaacaccg cgatgcccaa cagctcgccc gccgacacca gccgcgccgt
     3241 cagcacatga tcttcctgac tgggtgatgg atcgccactg ggatgattgt gtagaaaaat
     3301 cacggcggca gctgattcct tgaccgccat cgtgaacact tctcgcggat gcacaatact
     3361 gagtgtcagg ctgccttcgg agaccgtcat gtccctgatg atcgcatgct tggcgtccaa
     3421 cagcaccacc ttgaaaatct catgacgcag atctcgcaat gatggatggt agtgcgcgaa
     3481 cagctctcga ctggagccaa ttttcatgcc tgtggacagc ggcgaggcga gggaccgttt
     3541 gccgagttca atcgcggctt tgatttgggc cgccttcgcc tccccgactc caggcaccgc
     3601 gcagagttcc tcgatgccgc agcgggccaa tcctgacact ccgccgagac aatccaggac
     3661 ttccatgctg acctgcacgg cggagaagcg tgcccggcca acacgcagga gaatcgcgag
     3721 gagttgcgca tcggttaagg cttcggcgcc ctgcgtcaac agccgttcac gaggacgttc
     3781 ggtcacgggc cagtacgcaa tgccttgtcg cgccgccttt tgctccaccg atttctcctg
     3841 acaaaaatat gacacgttga acgtttttgt actagctgag acccctttga agaggcgctg
     3901 gtaacaaata cgtaccacct aagacgttga aattacgtat cgacaaaaat tggttcggct
     3961 cttgctttgg ttggccccca gctgtgcatg tcgcaaccag ggaggaagcg atggaatgtc
     4021 cgaagtgcaa agggctcatg atgctcgagc gattctccga cttctttctg attttctatg
     4081 catggaagtg cattaactgc ggtgcgatca ttgatcgcac gatttccaac aatcgtcgga
     4141 aaagcttggc cgcacgcgat gcgcaggcgg cccagccggc gcagccttcg gtcgccgcca
     4201 cacgctgacc ctctcgctcg cgcatccgga cgcgagcgct ggagtctcgg agtgcctctc
     4261 gtttcgactg catgaagccc ctctcacacg ctggcaccct cacggtccag aattatagtc
     4321 agcctcgggt acagcgtcaa aatgccagta atccagcccg caggtgtgtc gccgtccagc
     4381 cagcctctgc cgtgaaccga tcgagatgac atttctcgcg tcaccttgac ccctgcaaaa
     4441 acgctatgtt acagtctggc acgccagcga tgcctctcgc ctcaccagca tgccgataca
     4501 gtccggacgg tgccttccct gatagacggc ctggacatgc ctgttccgga gacccacggt
     4561 gaaccgagat gcatgtcgcg gcgcggctcg ctcggcctcg atgatcgact ccacggcccg
     4621 tgggtgatgc ggcgtgattc gcagagcgct gagtgactgg acaggatgaa acgaggagca
     4681 tgctgcgtcg ggtgcgcacg gagtgtcatg atcgtaagcc agtaaccgcg agtgatgaaa
     4741 tagcgtcgta aaattctatg cgagtcatcg ccggttccca tcgcggtcgg cgtctcaatg
     4801 cgccacaggg aaactccctg cgtcccacgt cggataaggt acgggaagcc ttgttttcca
     4861 tcctgggccc acgcgtatca ggcagccgct ttttggattt gtacgcgggc acgggagcgg
     4921 tgggcattga agcgttgagt cgaggcgcac tggtcgtcac gttcgtcgag tccgatccca
     4981 aagcgatgca ggccatgcac cacaacctga cgacctgtgg actgctcgag caggcggaat
     5041 tgcgcaggga gtcgacagac atgtttcttc gacagcggcg gtggcagcac gctccctatg
     5101 agatcgtctt cgccgatcct ccttatgcag atcctgaagc ggtcgaattg gtgctgaccg
     5161 gttggaccat cggacttctt gcacctgatg cggtgatgat catcgaacag gatgcgcggg
     5221 ctgagttgcc ggtcgccacc aatgacgcgc ggttcacacg tcgctatcgg tacggagaca
     5281 cggcactctt tctttacggg gcggccccgc gcgggttgcc ttcatgaaaa tcggcatcta
     5341 tcccggcacc tttgatccca tcactcatgg gcataccgac atcattaccc gtagcctgcg
     5401 ggtatttgat aaagtgatgg tggccgtggc cccgaatccg accaagcatc cgctcttcaa
     5461 cctggcggag cgactggaga tggtccagct ggtcttgaag gatatcggcc aggtcgaggt
     5521 cgtcaccttt gacggcttgt tagtcgacca tgtgcagcgt tccggcgccc atgccatcat
     5581 ccgcgggctg cgggcgatct cggattttga gcatgaattt caaatggctc tcatcaaccg
     5641 caaactggcc aaacaagttg aaacggtatt cctcatgccg agcgaagagt attcgtacct
     5701 gtcgtcgacg atcatcaagg acgtggcgaa ccacggcggc acgctgaccg agtttctcca
     5761 tcccgaagtc gcacgtcgcc tccaagccag aattcggagt gtcaagtcat gaagcttgcg
     5821 gcccgcgtca gtcggattgc gccctccccg accttggcca tggccgcaac tgccaaggca
     5881 atggcggcgc aaggcatcga tgtgatcgat ttttcgactg gagagccgga cttcgatacg
     5941 ccggagccgg tcaaagccgc tgcagaagcg gccatccggt cgggcttcac gaaatacacg
     6001 ccgtcatcgg gcatcgacga gctgcgcagc gcgatcgccg agaagctcaa aattgagcaa
     6061 ggcctgcaat acgacaaatc gcaaattttg gtgtcctgcg gcgcgaaaca ctctctctac
     6121 aatgtggccg aggcgttgct cgaagccggc gatgagatta tcatccccac tccctactgg
     6181 gtctcctatg cggaccaggc cttcttgaat gatgccaccc ccgtgttgtt cgccaccagg
     6241 gaagccgacg gctatgccat cgattccgcc gccttggagc aacgaattac gccaaggacg
     6301 aaagcgctca ttatcaatag tccgagcaat cccacgggag cgacgtacaa ccgtccgatg
     6361 ctggagcgga tcgcggcgat cgccgtccga cacgatcttc tcgtgatctc cgacgaaatt
     6421 tatgaaaaag tcctctacga tggagcgacg catgtcagca tcgcgtcatt agggccggac
     6481 attgccgccc gcaccgtggt catcaacgga gtgtccaagg cttatgcgat gaccggttgg
     6541 cgcatcgggt atgcggcagg tcccaaagag ctcattaccg cgatggggaa cattcagagc
     6601 cagagcacct cgaatccctg ttccatttcg cagaaggcgg cggtggcggc gttgcggttc
     6661 ggcgggccat tcacgaaaca gatggtgacg gagttcgatc gccggcggcg ggccattgtt
     6721 gaaggactca ataccattcc gggcatctcg tgttccatgc cggccggggc cttttacgca
     6781 ttccccaata tccagggggt cctgggcaag cggggaccga ccggtccgat tacgtcgccg
     6841 acggacctcg ccacgtatct cttaaaagac gcccacatcg cggtcgtgcc aggagaaccg
     6901 ttcggcagtc agtcacacat gcgattgtcg tatgccacca gcatggagac gatcggcaga
     6961 gggctggatc gtctgcgagc ggcgctgcaa aaactcggtt aagcgctgta tggccgtgtg
     7021 agaaaagcca gtatcattgc cggaggaggg accacccgtg ccgatttatg aatatcagtg
     7081 cgacagttgt tcgtatcagt tcgaagtcaa acagagcatg aaagatgctc cgttgacgac
     7141 ctgtgagcgc tgcgggaaaa gcctgcgccg gctcatctcc tcaccgggaa tcatgttcaa
     7201 gggaagtgga tggtatgtca ctgattattc gaacaagctg aaaccgccat cagatggtga
     7261 gaagcctgcg accggagaga aaaaagaaac gcctgccggc tctcctgcac ccgcgtcgac
     7321 ggcagcagct ccgaccggca ccagctcaac accggccagc acctcctcga ccagttcatc
     7381 ggccacaacg cccccgacga cgaacaagcc gaaaagttag cgcatcaggt cggtcgtgct
     7441 agtgcacggc cgacttcgtg agcgtcttct ttttgaccgg tgctttttgt gcagtcttga
     7501 ccgccggctt cggcgtcact ttggcaagag gcgccttagc ggccacgggt ttggatgatt
     7561 tagccaaccg ctcctgcagc gcactgacac tgctctgcag actggcaatc agcgtgctct
     7621 tgtctcgatt cgtgcggctg aggtcgtcga cttgcatttg caaatcgtcc ttcgcctttg
     7681 tgagcgatgt catttgattt tgcagctggg ccttctccat ggtgactgtc tggagctcag
     7741 accgaagttg ctcaatctgc gtttggagcg ccggcggcca gtaatcgcaa ccggccagca
     7801 gcaccgccgc tgtcatcacc agccctgccg ctcgccgcgg tcgacacgtg tcacggattg
     7861 gcatcgatcc ctcctttgta tgggcaccgc tgcctgctgt ataccacatt tacgaattct
     7921 tgagtgaaaa tcctcgctgg tgaagccttg cctctatcac gcttcgctcg atagcgccct
     7981 ctctcacgat gcgcagggaa tgatgggcgt ccaccacggt cgagggtaaa ccgccgggcg
     8041 tggggccggc gtcgaagatc agatccactg catctcctaa cgcctgctgc acagcctccg
     8101 ccgttcgggc cggtgccgcc ccggcacgat tcgcactcgt gcccgtcagg gggccgacac
     8161 gcgcgagcag ctcgcataag ggccggcacg aactgagtct cattcccacc aggcccgtgc
     8221 cggccgtgag agcacgcgga agagaggcgc gcgccgggaa tagaatggtc agcggaccag
     8281 gccaaaaccc ctcgattaac agcgaggccg ccgggggcac ctgttccacg aacgctgaca
     8341 ggtcgttgat cgaggccact aagacgagaa taggttttgc gtcttcgcga cctttcacgg
     8401 ctcgtaagcg atccagggct cgagcatcga agggattcac gccaagaccg tagaaggttt
     8461 ctgtggggat cgcgaccagg ccactgcgtt ggagcactga cgcgacctgt cgtgagagac
     8521 gctcgactgt cgaaggggaa taggactcaa tcggagccat gtgaaaaagg catcagcacg
     8581 tccggcctca gaccgggaag gaactgacct attccatcgg agaggaccag aggcatcagt
     8641 gccactccgt catcgagtga caagcgcacg atgggcgatg tcacgccggt aatgacaccc
     8701 cgtaaaggct atccgttcgg ctgtgcgata ggcaactgcc tgcgcctcgg ctaaggtgcc
     8761 gcctcgaccg gtgaccccaa gaactcgccc gccggctgtg acaatgtcat tgttgcgttt
     8821 ggcggtcccc gcgtggaaca ccacggcggc gtcctcgaag ggtgcatcta atccggtgat
     8881 ccgcatgcca ctctgatagg gaccaggata accggcggac gtcatcacga cgcacacagc
     8941 atgctcatcg tgccactcga cgcgaagctg gtccagccgg tgctctacga ccgcttccat
     9001 gacgtcgagc aggtcggttt tcagcagtgg caacaccacc tgggtttcgg gatcacccag
     9061 tcgagcatta aactccaaga cgtacggcac gccgccgacc accatcagcc ctgcatacaa
     9121 cacgccttga aacggagatc cgagtcgcga cattgcgtcc acgatcggct gcaaaatctc
     9181 tttcgtcacc tgcgcgcgaa gtgccggcgt gccgagcggc gccggacagt aagcccccat
     9241 cccgccggta tttaatcccg tgtctgcgtc ccccacccgc ttgtggtcct gcgccggcag
     9301 catcggtgcg acggaccggc cgtcggtgaa ggccataatc gtcaattcct cgccctcgag
     9361 aaactcttcg atgagcactt gcttcccggc ttcgccaaag accgcgtgct ccatcgaatc
     9421 gacgatcgcg tggcgggcct cctcccgcgt gagggcaatg agcaccccct tgccttgagc
     9481 cagaccatcg gccttgatga ccacgggcaa ctcgtggtcc tcgagatagg ccagcgcatc
     9541 ggccggcttg tcgaatgcct gcgaacgcgc cgtacgaatg tgggcctggg ccatgatcgc
     9601 tttggagaag atcttgctgg cttcgagccg ggctgcctgc ttggtggggc cgaaaatctt
     9661 gagcttggcc ttgcgaaatt cgtcgacaat cccgagcgcc agcggcgctt ctggtccgac
     9721 gacggtcaac tcaatgcctt cccgctgcgc gaacgttttc aatgcggcca gatcgttggc
     9781 gttgaccggc acacacgccg ccaacgtctc aatgccggcg tttcccggcg cacaaaagac
     9841 ctgcggccgc cggggactct gcacgagctt ccagacgagg gcatgttcac ggccgccgtt
     9901 gccaacgaca aggaccttca cgcaataacc ttgatgatgg ccgtcatgct cagatctcgc
     9961 ggtcgagcga tctgctcgtc tcaccatgac gctgagatgg acagcaacgg agaacgtcgc
    10021 gacgaatcat ttcagcagcc gactaatggc ggaagtggcg catacccgtc agaatcatcg
    10081 ccaacccatg ttcatcagcc gctttgatga cctcggcgtc gcgaatcgac ccgcccggtt
    10141 ggatgacagc agtgatcccg gcttgcgcag ccgcatcaag accgtcccgg aacggaaaaa
    10201 aggcgtcgga ggccatcacg caccctttca tgggaagttg ggccttcatt gcagccaact
    10261 tcacgctgtc cacccggctc atctgtcccg ccccgatgcc gaccgtctga cccggcttgg
    10321 catagataat ggcgttcgac ttgacgtgtt tgcacacctt ccacgcaaag gcgcaggcgg
    10381 tatactcctc gtccgtcggt ttgcggacag tgggtaccga cagcatccgc acgtccggca
    10441 acgtccctaa atcccgatcc tggacgatca gtccgcccac cagctttttg aaatcgtacc
    10501 cttcctgttt caccttggtg agcgcaccaa cgtccagaag ccgcaaatct ttttttcgcc
    10561 tcaattcggc gagcgcctct tcgctaaatc ccggggccac gaccacctca acgaaggtag
    10621 aggtgatttc cttcgctgcc ggaagatcca ccgcacgatt gaaagcaatc acgccgccga
    10681 acgccgatgt cggatcggtt tcgcgcgcct tcacgtatgc ttcaacagac gtcgccccca
    10741 acgcgacgcc gcacggattg ttgtgcttga tgatggccac ggcgatttcg tcgtattcct
    10801 ttgccaattc caacgcggca ttggcatcaa ggtaattgtt gtacgacatc gctttgccgt
    10861 gcaggatttt tccgtgcgag acggacggct cggtcgactg ccgttctcga tagaaggcgc
    10921 cttgttggtg tggattctcg ccatagcgca ggatttcccc caactcgtac tgcagggata
    10981 ggatcgccgg aaacttcgcc gctccgctct gcacctgttt ttccatgtag ccagcaatca
    11041 gactgtcgta acgggccgta tgctgaaaga ccttcatggc cagttcacgc cggagcgccc
    11101 gggtgaccgt attgtccttc aacgcttcga gcacgcgctg gtagtcggcc ggatcgacga
    11161 ccacgagcac atcttcatgg tttttggctg ccgaccggag catcgaaggc ccgccaatat
    11221 caatgttttc aatcgcctca tcgaagtgac aattcggttt ggcgatcgtc gcttcaaaag
    11281 gatagagatt gacgaccacc acatcaatcg ggccgatgtt gtgctgcttc atctgctcga
    11341 cgtgagctgg aattgaccgc cgccccagca atccgccgtg aattttcgga tggagggtct
    11401 tcacgcgacc gtcaagaatt tcaggcgatc cggtgtaggc cgccacttcc gtcaccatga
    11461 tgccggcatc ccgaagcgct ttcgctgttc cgccagtcga caaaatttcg gcgccaaggg
    11521 ccgtaaggcc ctgggccatg tcgaccacgc cggtcttatc cgagacactg atcaatgccc
    11581 gcttcatgct ggccatggga gcactccttt agaatgattg gcgacttgga agggacgggc
    11641 ggaggatagc aaacgatggg tccaagttca acacgccagc tttccctggg gccatactcc
    11701 gggctggctt gcgtccgaca gttatgatac ggtgccattc aagtgatgct tgcggttgac
    11761 tggctgaagc gcatggaacg caggacgatt ctgggtggcg gattcttcgc tctgacactg
    11821 ctggccctcg tgtgtatccc tcgtcacctc ccgtcgtcgg cctcagctcc cccgccggtg
    11881 ccggcaaact ttcacgcaca ccttgaacat ggagcgcttc tccttcgcgg ttcgctcccg
    11941 aatgcaggcg cccatgaggg gattcttcga caggcgcgtg ccgcctacga tcccacgcag
    12001 gtccgtattg tcgatcaact caccgtagac agccgaatcg cctcgccgcc ctgggtgaat
    12061 caactgcccg ccatcctgcc cgtcttagaa ccgatgcaag gggcgcgatc ggtcatgatc
    12121 gatggccgct tcgtcgtcct gagcggcgct gtgccctccc agcaaatcaa ggccaaaatc
    12181 ttgaacaccg tgtcgccgct gcgcggactc ggcctggaac tcgaagacca tatgaccatc
    12241 gccggtccgg cggcctctgc ctcactccag gcccgagtgg atgaactgct ctctcacagc
    12301 cagatcgaat tcgaatccaa ccaggcaacg ctgacggctc gaggtcaggc ggtcctcgat
    12361 cggctcattc ccgttttgaa ggaagcgcca cgcacctcaa tcgagatcgg cggccatacg
    12421 gacgggttcg gggcggccga ctataataag gagttgagcc gccgtcgggc cgaggccgtg
    12481 cggcactatc tgatggcaca tggcgttaca catgagctga gcgctgtcgg ctacggtgcc
    12541 agcaaacccc tcgccgccgg gatgacgcga gccgaacttc aacgcaaccg gcgtatagag
    12601 cttcgtgtgc tggagacgaa cgggtgatga tcggactcat tactcaaatc ctgggatgtc
    12661 ttctcgtggc cgccgccatc ggcttcgctg tggggtggct cctccggaac cggtccgcag
    12721 agtcggacga gcatcagctg accgaccgag agacggaact gcgcattaaa gggcaggcgc
    12781 tcgacaccgc gatctacgaa ctcaaggtga agacgtcggc gctgtcggcc ttggagtcga
    12841 aagtctcttc gctcgagagt ttgggtcgct cagcccagca cgagctggcg tcccgccagg
    12901 aacgcattga ccggctgcaa caggagctca aggagagcca agctcgcgtg gcgtctttag
    12961 agacggagca acaagccagg cgtgaacggc tcgccgaaca ggacacgatg ctctctgcgt
    13021 acgcgaacga agctcgccaa gccaatgcag cccgtacaga atcgcaggag caactcgcgc
    13081 tcaaagaaca ggaactcctg gaactgcagc aacgcttggt cgagtcagac aacaggcttg
    13141 ccgagattga tcggctcagg agccagctgg cggagctcga accggctcaa gggcgtgtcc
    13201 actggttgga agtccaacta agcgaaaagg acgcgcagca tcgacgggcg ttgcacgccc
    13261 tcgaagatga tctctccgca cgggatcgac gtcttgccga actggcgcaa caggcgcagc
    13321 tgctccacga tcgcgaccgt cagatcgccg gactggagct gcgtgtcgca caactgcagg
    13381 ctctgcaagc cgagatggcc ggtcaggcaa agacgatggg cgagcaggag gctgaaattt
    13441 cccggctgcg taaacggctc ggtgaattcc gtgcagccct tcgtggacgc gacgacggca
    13501 gccccgtgct tgtccgtccg aacgggccgg ccaaccaatt gactctccag atcccctcgc
    13561 cgaaagcctc cacttcgcca cgcaaagacg acctcaaaaa aatccccggg atcggtcccg
    13621 ccatcgaacg ggcgttgaac aagatgggga cgttcacgta tgtccagatc gccaggtgga
    13681 aagacacgga tattgcccgc gtggcgcgaa aactggacac ggtgcctggt cggatcaagc
    13741 gggacaattg gatcgcaggc gcgaaaagac agcatcgcga gaaatacggc gaaaaactgt
    13801 agcgcccgcg ccgtcgcgtg aacctgtgcg ttgacttgct gccatcgccc ccctagaatc
    13861 cgccgtcatg agtgcttcct tcgtgcacct gcatttgcac acccaattca gtcttctcga
    13921 cggcgccaac caaattgaac cgctcataca gcgagtgaaa gccttcggac agccggccgt
    13981 ggcgatgacg gatcacggca acatgttcgg cgccatcgag ttttaccgaa aagcgaaaga
    14041 acacggcatc aagcccatca tcggctgcga agcctatatg gcactgggca gccgccacgc
    14101 caagaaagac agcggactcg cccacaacga ttattaccac ctcatcctgc tcgccaggaa
    14161 tgtgaccggc tatcaaaatc tgatcaaact cgtcagtaaa gcatatcttg aaggattcta
    14221 ctataagcca cggatggaca aggagctgtt aaaagaacac catgagggtc tcattgccct
    14281 ctcggggtgt ctgagtggag aaattcctta tttgataggc caaaaagaca tgaaggctgc
    14341 gctcgcggtg gcccaagaat tccaggagat cttcggccgg gatcagtttt acctggaagt
    14401 gcaagccaat gggctggatc accagcggat tgcgaatgcg gggctgattg aaatccataa
    14461 acagctcggc attccgctcg ccggaacgaa cgactgtcat tatctcaaaa aagaagacgc
    14521 caggccgcac gaactcatgc tgtgcctgca aacgggcaag accctgagtg atccgaccag
    14581 aatgaaattc gacaccgacc agctatacgt caagtcgacg gaagaaatcg cccccgcgtt
    14641 cgccgagttc cccggagcgg tggccaacac ctgccggatc gccgacgatt gcgagctgga
    14701 gctggcactc aacacgaccc atctcccgca atacaaagcc ccggagggct atacccgcga
    14761 atcgtatgtg gagcacctcg ccgtcgaagg cctcaaatcc cgtttgagcg aacgcccgag
    14821 tcggattgcc ctggcggtct acgaacaacg gttgcgcgaa gaattgatgg tcatctgctc
    14881 gatgggattt gccgggtact ttttgatcgt gtgggatatc atccggtttg cgcgttcacg
    14941 tggcatcccc gtcggcccag gccgaggatc ggcggcaggc agtctcgtgg cctacgcgct
    15001 gcgcatcacc gacctcgacc cgctcgtcta caccttgctc tttgaacggt tcctcaatcc
    15061 cgagcgcgtc tccctaccgg atatcgacat ggacttctgc atggaccggc ggggggaggt
    15121 cctcaactat gtcgtggaca agtacggttc cgatcacgtc gcgcagatca tcacgtttgg
    15181 aaccctcggt gcgaaggcgg ccatccgtga cgtcggtcgg gtgctcgaaa tgccttatgc
    15241 agaagccgac aaggtggcca agctggtgcc caaccaactc aacatcacac tcgagcaagc
    15301 gctcgaaagt gaaccgcggc tccgcgaact ggtcgacacg gatccgaagg tgcgagagct
    15361 catgagcatt gcccgatcgc tcgaagggct tgcccggcat gcctccaccc atgcagccgg
    15421 cgtggtcatc tcagaaggcc ccctgacgga ccacgtccct ctgtacaaag gcgccaatga
    15481 cgaaatcgtg acgcaatatt ccatgggaga tgtcgagaag atcggcttgg tgaaattcga
    15541 tttcctcggc ctgaagacgc tcacgatgat ccggcgtgcg gaatcactga tcaacgaaag
    15601 ccgccccgat cagacgcccc tggtcgtcga ccggctacca ttcgacgacg ccaagacatt
    15661 tgccctgctg tcgtccggaa aaaccaccgg gattttccag ctcgaaagct ccgggatgcg
    15721 cgacctgctg accggattca aacccgaccg cttcgaagac atcatcgcga tcatcgccct
    15781 ctaccggccg ggtccgatgg atctgattcc cgacttcatc aagcggaagc aggggaaaat
    15841 tcctatcgtc tatgagttgc cggagctcga gccgatcctc aaggatacct acggggtgat
    15901 tgtgtatcag gaacaggtga tggccatcgc caaccgagtg gccggatttt cactcggcca
    15961 ggcggacatc ctccggcgtg cgatgggcaa gaaaaagccc gaagaaatgg aaaagctgcg
    16021 cgtcaagttc gtcgacggcg cccgccagaa caagatcgcg gacaggaaag cggagaagct
    16081 gtacgagttg atccaaaaat tcgccggcta cggcttcaat aagtcgcatg ccgccgccta
    16141 tgccgtcgtc tgctatcaga ccggttacct caaggcccat tatccgaccg agttcatggc
    16201 cgccctcatg acgagtgata tgggaaatca agacaagatt atggggtatt tttcagaatg
    16261 ccgggatctc ggcatcaaag tgctgggccc cgacgtcaac gagagtcaga aaaattttgc
    16321 ggtggtggac ggcgccatcc ggttcggact tgccgcgatc aagaacgtag gggaaggtgc
    16381 ggtggactcg gtcttggcca ttcgcaccga aggcggacgc tttacctcgt tctttgagtt
    16441 ctgccggcgc gtcgatctcc acaaggtcaa caagcggatg ctggaagggt tgatcaaggc
    16501 cggcgcattt gattccttcg gcgccaggcg cgcgcaattg atggcgatgc tcgatcaggc
    16561 cgtcgaagac ggcgcggcgg cgcaacggga acgcgacctg ggtcagacga acattttcgg
    16621 tgaagacgtg cacggtggcg agcattccac ggctgccgtc gaaccggcct taccgaacct
    16681 gcccgaatgg gaccaggcgc aacggctcaa gtacgaacgg gagctgaccg gtttttatat
    16741 caccgcccat ccgctggccc gctacgaagc gaccatgcac gcgctcgcca cggcaacgac
    16801 ggcgggcatg acggaattct ccgacggcaa ggaagtgcgc ctgtgcggga tcattgccgc
    16861 agtgaaatcg atgctgacga aaaagggcga tcgaatggcg tacgtcaccc tcgaagatct
    16921 gcagggcact gttgagatca tcgtgtttcc ggacctcttc aaaaccgccg gcgagctgat
    16981 cacccccgag cggctcgtgc gcatcaccgg caccatcgac cggggcgaca agggcaccaa
    17041 agtccgcggc acgaagatcg aaccgctcgc tgacgtgcag acgcaaacga tcaaacgcat
    17101 ccacattcgg ctggcggcgt gccccgaggc gaaggatcaa ctgccgaggc tgctcgatgt
    17161 attcaaacgc catccagggg gcaccgcgat ctccttcact tttcggacgg atggcttgct
    17221 ggaagccgaa acggctcccc ttcccaatct gagggtgaac gccagtgaac actttgtggc
    17281 agatgttgaa gaagtgctag gcaaaggggc cctttctctg gtatcgtagc gaaccgacaa
    17341 tggggtgggc tcccactccc gcctcccgac caacccgatc gacacactcg atgcgcgact
    17401 atcttgagtt tgaaaagccc ctccgcgagg tggaagaaaa gatcgaaaag ctggcggcct
    17461 cgggcggcgg gaaatccggc gtgcaggacg aaatccgcaa gctccggacg aaattagcgc
    17521 agttggagca cgaactatat agcaagcttt ctccgtggca gcgcacccaa ctcgcccgac
    17581 atccgcagcg gcccagcacc ctcgactata tcggtgagat gtttcgcgac tttctcgaat
    17641 tccatggcga ccgctcgttc ggcgacgacc gcgcgatcgt cggcggcttt gccagcttca
    17701 acggccggtc cgtaatggtc attggacatc agaagggcaa aacgctcaaa gaacggatgc
    17761 agcggaactt cggtatgccg aacccagagg gctaccggaa agcgttgcga ttgatgaaaa
    17821 tggcggagaa attcggccgt cccatcatca ccttcatcga tacgccggga gcctatcctg
    17881 ggatcggcgc ggaagagcga ggacaagccg aagctatcgc cagaaacctc tatgccatgt
    17941 cccgcctcag cgtgcccatc gtctcgatcg tcatcggcga aggcggtagc ggaggggcac
    18001 tcgccctcgg cgtatcggat cgtatcttga tgctggagca cggtgtgtat tcggtgattt
    18061 ctcccgaagg ctgtgcggcg atcttgtggg acgatccggc gaaagtgccc gacgcggccg
    18121 cggcgctcaa aatgaccgcc caagacctgg tcgatcttga aattgttgac gaagtcattc
    18181 ccgaaccgct cggcggtgca catcgcgaac cgcgagccgt caccgatcgg gtcgccaagg
    18241 ccttgacgaa ccaactctat caattgaccg atctttctgt cgccgacctc ctggaccagc
    18301 gtgaccgaaa gtaccgtcgc atcggggccg tcactggtct cgatcccagc gtcaattaac
    18361 cggcggctga cagccgtcga cttcttcccc ccattcgtcc ttcgccgctc ctcgtcacca
    18421 ccttcatgca ctgtggcagt cgccagaccg cttggaaacc ccaattcgtt gacaggcagc
    18481 tgacgactgc tatgatgccg ggaagaagag ggaattccct cggtagtggc ttcatgacct
    18541 ctgtctcgat cgcccaatgg aagctcgaaa gttccagcgg ttttcagatc attcgcatgt
    18601 ggcgttttcg agtgaaaccg tcaaaggcga aggacgcctg gagaatctat cgctcggcgg
    18661 ggcagcgatt ctgagcgacc tcaccattgc ccgaggggaa tatttcgcgc tgagaatcac
    18721 cttccccacc caggccacgg caatcgagat cgaacttgcc cccgtacgct gggttaagcc
    18781 gggcagtttc ggcgtggaat tcatccgtat cgcgcccagc tcacagcagc atctcaagca
    18841 atatctcttg acgctcgaaa acgcctcctc tgacgccgct taaccgttgg ctgagaaacc
    18901 acggaggtat tgtcacagcc tcaatggcct ccgccggggt cgtttccctg caaggaaaca
    18961 atcgagcgta tggtcattga ccatacccac cgcttgcatg aaggcgtaca tgatcgtact
    19021 gccgacaaac cgaaaccccc gcttcttgag atccttcgac agtctatcgc tctctggact
    19081 cgtggccggg acgtccgacg agcgcttcca gccattgaca atgggggcgc cttccacgaa
    19141 ctgccaaaca tagcgatcaa acgatccaaa ctcgttctgg accgccagga aggcccgggc
    19201 gttgctcacg gcagcttcaa tttttaaacg gtttcggata atgccgacat ctctgaggag
    19261 cgcggcttgc ttgtctcggc cgaatgtcgc caccttgacc ggatcgaaac cggcaaaggc
    19321 tcgccgatag ccttcccgac gccgcaagac ggtttcccag ctcaatcccg cttgggcccc
    19381 ttccaagagc aacatctcga agagccgtcg atcgtcatga acgggcttcc cccattccct
    19441 gtcgtgataa cgccgcatgt ggggctgtga accagcccag acgcatcgag gcatgttcct
    19501 ctctccctta gacatgggtg tggctcccaa gggaaatccc ttgcgataac atcacgagga
    19561 cggtcacaag caccgatttt gccgtgcctg cttcctgccg actggtctct ttcctagcgt
    19621 gaacatgttc aactccattc tatagtagga gacagggcgt cttcgcgact cagtttcctc
    19681 tggtctcata accagcgtca ggaacctggt gaactaccct tgaaacacac ggattgtcca
    19741 tcgccgtctc tgcggtcgcc gatggcagag gagtttgggc atggcgcaaa ttctttagac
    19801 tccgtcgact caccaaggag ggaaacgatg aaatatcaac tgatacctgc ctgccttgtg
    19861 atgctggggc tcacggcatg tagccatacc atacccgaga cctataccaa accccaggcg
    19921 ccgcctcagt tgggcaagac cccgccggtc atccaaccgc tgaatgccac tcaaaagcag
    19981 gcgcaggccc cggaatcgct actccccgtc acgatgacag ccgacttgac gccggtgcaa
    20041 aagaccatcc aagcggcgct gccagaacgg ttcaccgaag agggtcaccc gttggccaaa
    20101 gactatcgct ggcgcttcat ccgggaaggc gagccgcagg tgcagatcca ggatggcttg
    20161 gtgacattta gcgccaccta tcgaggcgag atcgaatccg ctgctgcccg cgcctgccgg
    20221 ctcgatccct tgtacccggt tctggagggg acaggacgac tcgagttacg tgagcaggac
    20281 caagggctgc ttgtcaccat gagccagccg caaacctcga tgaatctgaa gccggagagc
    20341 gatacgaaat gcaacatgtt caacctgccg gtcaaagatc agctggccga actcttccag
    20401 caggaatcag tgaggcaaca catcgtgcaa tcgctcgagc aggccggcta cgtcattccg
    20461 ctgaacctga tctgggaccg tctgcaagag cccatgccgg tcagttcagg aaacaccaaa
    20521 ctctgcctgt acgggaaagc caaggatttt gccatcggcg ccctcaaggg tccggtgcag
    20581 aatacgacga tcacgggcgt cgtccggcaa acaccggtcg cgctcttcca gacgccttgt
    20641 caaaaaccag gctcgacgcc cttgaagatg catatggatt cggccgcaac ggccatgcag
    20701 gaaggtcaac cttacagaat ccttttgagc gttcctgttc cctatgccgt ccttaatcag
    20761 caacttcaag agcggttgta tcatcaggga gtgaagcttc cgactacctt cgacaatgac
    20821 ttgacgatcg aacgggtgac cgcctccgac gtaaccggtc ggacgctgtt cgccgtcgaa
    20881 accagcgggg acacgaaggg gacgctctac tattggggca cgcctcaatt ggagcaagag
    20941 ggcaatatga tcacgatccc tgatctccaa atcgccaacg aaacaaagat tgcgctcgaa
    21001 gagatcaagt ccggctactg gcaaatggtg gatgatcaac tgaagccgcg cctgcgacag
    21061 gcctcaaccc tcgatctctc acagcggatc ggaaccatga ggagtgcgct gagcggccag
    21121 cataaatccg gaagcgtggc catggatctg ctcgtcgcca agcaggaagc cggacaggtg
    21181 gtgtcgacca aagatgcgct cgttgccgac gtgtttctag aagggaccgc gagcgccgca
    21241 ggacggctgc ccgtgaagca ggcttcacgc ggtgacatcg atcagaatcg tccggtggcc
    21301 acggaccgaa ctccggcggc cgaggctgcg ccaaaagcgg cccgtaatcc cgacgaagaa
    21361 cggcggtagt tgtttttgat attcacgggc atggtcagga acgcgagtcg ccgtgggcct
    21421 tgtgtgtccg ccctcggcga cgaggcggtc ctgaccctgc gcccctacca ggcataggcc
    21481 tccggggccg ggcccccagg tccgggaaaa atcccatcca atcgcttgag ggtctcggtc
    21541 gctaaggtca gattcatcac gcgcatcgtg ctcaccagtt gatcgagtgt gcgcggcccg
    21601 atgatcggcg aggtcaccgc cggctgatgc agcagccacg cgatggccac gtcggctggc
    21661 cgttcaccga gttcatgaca cagcttctca taggcctcca gtctcggacg atacttctcc
    21721 acctcctgct gcacctcctt gtcggcacgg cggcccacgg tggagggcgt caacgcgcca
    21781 gccagcagtc ccctcgcaag ggggctccag ggaatcatcc cgatgccata cgctgcacag
    21841 gcgggcagca cttcaagctc gatcgtacgt tcattcaaat tgtacaggct ttgttcggag
    21901 acgattccaa ggaagtgacg gctgcgcgcg agttcctggg cctgcgcaag ttgccagccc
    21961 gcgaagttgc tggtcccgac atagagaatt ttcccttcgc gaaccagttg ctccatcgct
    22021 tgccagattt cgtcccacgg cgtgttccgg tccacatggt gcatctgata gagatcgata
    22081 cagtcggtct gcagacggcg cagactcccc tcgcaggcac gtttgatgtg cagcgccgac
    22141 agccgcgctt ggttcggcca atcgcccatc cgcccataca gcttcgtcgc caacacgact
    22201 ttttcccgac ggccgccgcc ttgcgcgaac caacggccga tgatctgttc ggtccagcct
    22261 tcgccgactt tccagccata aacattcgcc gtgtcaaaaa aattgatgcc cagctcaaga
    22321 gcccggtcca tgatggccac gctgtcggct tcggtcgtct cgggaccaaa attcatcgtg
    22381 ccgaggcaca gccggcttac cttcacgcct gatcgaccca gatgaacata gtgcatcacg
    22441 accccagatc cgcgaacgct gcagcgcggc gtaacgtaca cgcctccggt taaagcccga
    22501 tcgtttcgat ccgctccagc tcgtccggcg tgagcgtgaa gcggtcgcat tgcagatccg
    22561 ccgtcatatg ctcgcggttc gtcgtgccgg tcagaggcag cataccgacc tgcatggcga
    22621 agcgaaacac aatctgcgcc acaccggtgc cgtatttctc cgccatggcg cggatgcccg
    22681 gctcggcaaa cacttcccga ttggcggtca acagcgaaaa gccctgatac acgatgtgtt
    22741 gggcccggca cagatcacgt accgccttgt cccatccgaa tgcggcgtag cagcggtttt
    22801 ggaccaccat cggtcgatgt gtcgcccgtt cacacaacag cgccaactgg ccggccgtca
    22861 cattgctcac gccgatgagc ctgctcttgc cgctggcata gagtgattca atggccgacc
    22921 acacttccca atcttccggc cccaatccgc ttcgagagta cggcccgtgg aggacatagg
    22981 aatcgaggta atccgtgcgt aaatgggtga gcgaactttc gaacgattgg cgaacttgcg
    23041 tcgtaatcgg cgctcgcgcg tcgtagggag ttcgatggtc ctgcccgttg acgggcgtga
    23101 atttggtttg gaggaacagc cggtctctcg tatgtccacc ctgcgccaag ctcactaacg
    23161 cctcgcccac ccgcgcctcg tcgtaatgga tgagctgatt ggccgtatcg atggccgaaa
    23221 agccggcggt gatggcttcg atcacgagct gggtcgtcgc ctccttcttc caggccgttc
    23281 catacatgaa ggccggcatt gttacgccat tatatgcgga aagcgtcatc gctccatgtg
    23341 ctccgggtgg aatagtatga ccacccgttg aatgtgagtg cgcacctcgc ccaccataca
    23401 cagattccta tcgcagtctc aatgacggaa ttggtacagt gcctcggatg aaccctgaga
    23461 gtgagatcgt cgagttgcgc ttcagctatc gttacatgaa gcagcacgcc tgggtggtgc
    23521 aggccgtgaa tggattcctg tcggcctatt tcatggagca gcccggcttt cgcgtgcagc
    23581 ggcattttga tgaactggaa tccggcatgc atgtatggct ctgcgaggta ccggcggcca
    23641 tgaagatgac ccggctcctc aaacgtctca aagcggacat tccacccttt cgccagacgg
    23701 agacacggtc ttcagccgac agccgtgcac agtatgtcat cgactcgcca gaccccgact
    23761 cgccagcgta ggcactcctt cgcgcaattt ctccgagacc tgttcaaacc ggcggacaag
    23821 agtatactaa tggaaaaggg aggcttgtgc gatgcatcgg gtcctggtcc tgggcgcggg
    23881 gaagatcggc tccttggtcg cctgcctgct ctcacaacag ggcgcctatg aggtccacct
    23941 cgccgacctc acgctcgacg caccgaagca tctggtggag gacctcgggc tctcccgcat
    24001 cacccctgcc gtcctcgatg tgcgccatcc cgaaaccgtc gaaacctatt tatccgctca
    24061 ccccgtggat gccatcattt ccagcctccc ctatttctgt aatcccaccg tcgcggcgct
    24121 tgccctgaag caccggtgcc attatttcga cctgacggaa gacgtggagg tcacgaatca
    24181 catccgcgcc ctgagcgcag gagccgcaca cgccttcatg ccacagtgcg gtctggcacc
    24241 ggggtttatc agcatcgtga cccacgaact gatgacccat ttcgaaacgc ttgatcacgt
    24301 caaaatgcgc gtcggcgctc tgccggttca tcccagcaac gcactgaagt attcgctgac
    24361 ctggtcgacg gatggcctga tcaacgaata cggcaatctc tgttacggga tcgaagacgg
    24421 ccgtaagatt ccacttcaac ctttggaagg ctacgaaacc atcgagctcg acggattgtt
    24481 atacgaggcc ttcaatacgt cgggaggttt ggggacgctc gccgacagct cggtcggctc
    24541 ggtgaagacc atgaattaca aaacgcttcg ttatcccggc cattgtgaaa aaatccagct
    24601 cctgatgaaa gacctcaagc tcaacgagga tcgtaagacc ctcaagcgtg tcctggagca
    24661 tgccattccg cagacgctcc aggatgtcgt gctgatctat gcgtcggtga cgggcacgaa
    24721 acacggcgag tttttcgaag agaactacgt caagaaaatc tatcctcaat gcatcaaggg
    24781 caagttgtgg tcggcgattc aggtcaccac ggcctcgagc gtgtgttgcg tcatggacat
    24841 cgtcctgcgc aatggggcgg cataccgggg cttcgtcaca caggaatcca tcgggctgca
    24901 ggactttctg gccaacgact tcggcgcgtg tttccgatga gtaccgtgca cgcagcgctc
    24961 aaagatttgg gaatccaggc cgtcaactcc ggcggcagca cgggcaagca ctggtggtcg
    25021 gaccatgcga ccggttcact gatccagtcc gtgaatccgg caaccggtga acccatcgcc
    25081 gcggtccgtt catgctcggc aggtgactac gagcacatcg cgactcaagc ccgtgacgtg
    25141 tttgccacgt ggcgcatggt acccgctccc aaacgcggag aagtggttcg tctgatcgga
    25201 cacgccttgc ggagcaagaa ggatcaactc ggcactcttg tgtcccttga agtcggcaag
    25261 atcaaggccg agggagatgg ggaagtgcag gagatgatcg acatggccga ctttgccgtc
    25321 gggcaatcac gcatgctgta tggcgcgacg atgcagtccg agcgaccgtc tcatcgcatg
    25381 agcgagcagt ggcatccgct cggtcccgtc ggggtcatca cggcgtttaa ttttccggtc
    25441 gcggtgtggg catggaatgc attcctcgcc gccatcgccg gtgatccggt catctggaaa
    25501 ccctcgccca aggcgccgct gtgcgcgatt gccacgcagc agatttgcaa ccaggtcatg
    25561 gagcagcaag ggtattccgg aatcttttcg ctgttcatca ccgatcaggc gtcacttgcg
    25621 gaaaccatgg ttcgggatcc gcggttgccg ttgatctctt ttaccgggtc cgtaccggtc
    25681 ggccgccacg tggcctccat cgtcgggcaa cggttgggtc ggacactgct cgaactcagc
    25741 ggcaataacg ccgtcatcgt ggatcagagt gcagacctcg acctggcagt acgggcgatt
    25801 gtctttggag cggtggggac ggcgggccag cgctgcacga cgacgcggcg tctgctcgtg
    25861 cacgagtcat gcgctgacac actccttgac cgtttagtga atgcctataa acaggtgcgc
    25921 atcggcaatc cattggattc cgacgtcctg ctcgggccgc tcatcgatca cgtggccgtg
    25981 gaagcctacc gcgcggccat cgacgaaatc aaaaaggaag gcggagagat tctttacggc
    26041 ggccgtgtcc tgacaaggcc cggctatttc gtggaaccga cgatcgtccg cgcgcaaccc
    26101 cactggccgc tcttgcaacg ggaaaccttc gctcccattc tttatgcgat gacgttccgg
    26161 acgctcgagg aagccatcgc catccagaat ggggtcccgc aaggtttgtc gtccgccatg
    26221 tttacgaaca atctgctcca cagcgagcgc ttcctctcgg cgtcagggag tgattgcggc
    26281 attgcgaata tcaatcttgg cacttcgggt gcagagatcg gcggcgcgtt cggtggagag
    26341 aaggaaacgg gcggcggtcg cgaagccggg tcggatgcct ggaaagccta tatgcggcgt
    26401 cagaccaaca cggtaaattg gggcacggat ctgccgctcg cacagggcat caagtttgga
    26461 tgatcggcct ttgggcaaaa gggacgccgt catggagaca aatcgtgaac tcgatgggtt
    26521 actggaacga atggtcgtgg aatatgtcgc ccgcaatcgg gcggccaaga ttctcaaagg
    26581 cctgctcgac gaggccggag tggggttcta cccagtcgtc gaccacgtga cgttacgcac
    26641 gatggatatc gaccggcgag ccgaaccctt cctggcactt gggtatgcct atgacgaaac
    26701 gctcaattac gacgattggt acgcgaaggt ctatcgcaaa gccggctatc ccgccttgtt
    26761 cgtggatcag gcctatgcgg atgcccgcgg gaagaccagt atcatccccg gctgggtcgc
    26821 cgcgttcgga gaccagatct ttcaccatat tgccgtgcgg gtcgaggaca tcgataaggc
    26881 ggtgaaccgg ctcaagacga acggagtggt attcgcgggg cagatcgtcg gtccggcggg
    26941 cggccagtta cggcagattt tttctgcccc cgaagtcgtc gatgggcagc ccttcaccgt
    27001 gctggaactc gccgaacgac accgtggcta cttgggattt ctgccgcccc aagccgatag
    27061 cttgatgaaa tcaacggccc ctcaataacg ttatggggcc tcccgactgt gacccgcctg
    27121 cgccgctgcg tctgagttct gcaagaacgt cgggccctcg atcggaagcc ggatctggaa
    27181 cgtggtcccc tgcccaggct cgctttcgac ggaaatctgt cctccgtgca tgtcgaccac
    27241 ttccttgatg atcttcgtgc ccagtccggt cccgccggcc ttcgtgctca cgacccgata
    27301 ggtgaagaga ttatcgcgca ggtcggcggg catgccgacc cccgtgtcgg aaacccagag
    27361 cgtcacgcag tcgtcaccgg cagcggacgc tcccttgatc gtgaccgagc cgtgaggcgg
    27421 cacttcggga atggcattat tgaccaggtt gtacaacagt gaatacaaac gcgcctcgtc
    27481 tcccacaatc gccggtaagg cgtgcagccc atcagtcttc aagacgacct gtttacgttc
    27541 ggccggtgca cgcaacgatt tcacaacact gtccgcgatc tttccgatca cacaggattc
    27601 aaatttttga gatacccgcg taagcgctgc gtaatccgcg atggctttca tgcggtcctg
    27661 aatccgccgt gatgtattac ggaacatgct aatgacttca tcgcaggcca ggtgactttg
    27721 ctcagcctga gccggcgccg gcgtcggaag atgtttgaat acatcatcga tctcttccgc
    27781 gagaaggtcg gtgccggcga cgaggggcat caagagattc ttgacgtcat gggtaatttc
    27841 acccatcaag cgcgtgatct cgacccgttg ccccatgcgc gcaatgagcg tcgtcaccgt
    27901 ctgatcgatt tgtccctgtt cgaacggctt cgtaatgaaa tcgacggcgc cttctttcat
    27961 tgcctccacg gcgaggcgga tcgtgccgta ggcggtcacc acgacgaccg ggagatccgg
    28021 ccatcgtttt ctgacgattt tgagcgcctc gatgccattc agacgcggca tcgagacatc
    28081 gaagaacgcc aggtcaaggg cgtgcgactc gaccgcagcc aaggccgcct gaccgtctgc
    28141 tgcggtgacc acatcgtgcc ccaaccagcg aagccgctca gacaggctca tgacaatgtc
    28201 ggggtcgtcg tcagcgacca gaatgcgaat gcccattgcg ctcccctttc aggcggtgga
    28261 ctcagcaaga aatgcgccat ggtatcggcc gccgatgtga cgagaattgc aggaagaatt
    28321 cccggtgaat ccttacggag ttgaatcaaa ttagacgaag atacggatca aaaccgatac
    28381 gctatcccat gtccttcaag acatggtcag gattgcggtc ccgtgcaatc gttttcatga
    28441 ggtgatcccc gaacagaacg accacccgcc cgcgctgatc cttgacgatc attgatgcgg
    28501 acggacgctt gaaggtttca gggtcgcctt ccaaccaggc actgcaggtg aacggtaagg
    28561 tatcaagcac cgtctcgacg cgatactcat gacgcagtcg atactgcagg acgtcgaact
    28621 ggagccggcc gacggcagcc acgagcgatt ccaagtcatt gaggctccgc atgatctgca
    28681 ccgtcccttc ctgcgccatc tgctccattc ccttgtcgaa cgacttccgc ttgccgacat
    28741 cggtcggacg gatgcgcgca aatatctccg gttgaaattg cggcaagggt ttgaagcgaa
    28801 agccgcccgt cacggaaatg gtgtcgccga tggcgaagac acctggattg ataatgccga
    28861 tgatgtcgcc agggaatgcc tcttcgaccg tgctgcgctc ttgcgcgacg agactgtgcg
    28921 gccgggacaa acgaacgtca cgaccgagac gatgatgctt cacgaccatg tcccgctcga
    28981 accgcccgga gcacacacgg agaaaagcgg tgctgtcccg gtgcttcggg ttcatgttgg
    29041 cttggagctt gaagacgtag gcgctaaacg gttgttcgat cggatcaatg gactgctctc
    29101 ccccgtcgtc tgtatccgcc aaccgggcac cgggagccgg cgccagctga acgaaggcat
    29161 ccagaaagct ctcgacgccg aaattcgtca gcgcggaggc aaagaacacc ggcgtgacct
    29221 ccccgcctaa gaactgttca tgggagaggg ggttgccggc aatggtgagc agttcgagat
    29281 cgtgctggac ctgtgtcatc acctcaggag aaacacgccc gcttttggcc aagtcctcca
    29341 atgccatcgt ctgcacgtcc acttttgtgg cgccgccgtg cgccgttttg ctgaatagcg
    29401 caacgcggtt gtcggctcga gtcacgatgc cagcgaagtc gcttcccgat ccaatcggcc
    29461 agttgacggc gctggcatga atcgtcaacg cctgttcgac ttccgtcatc aagtccaagg
    29521 gcggacggcc gggtaaatcc atcttgttca cgagcgtcag aaccggaata cggcgcagtc
    29581 ggcagacggc aaagagcttg cgcgtttgag cttcgacgcc ttttgccgcg tcgataacca
    29641 tgatcgcgct gtctgcggcc gtgagggttc gataggtgtc ttccgaaaaa tcttggtggc
    29701 ccggcgtgtc gagcagattg ataatggcct ctttgtaggg gaactgcatc gctgaagccg
    29761 tgatggagat gccccgctcc tgctccatgc ccatccagtc cgaggcggcc gtttttccac
    29821 ctttgcgccc ccggaccatg ccggcggtcc ggatcaggcc ggagtacagg aggagctttt
    29881 ccgtaagggt cgtctttccg gcatccgggt ggctgataat ggcaaaggtc cgtcgttttg
    29941 cggtggcgat tgccaattcg ttgtgctcgg tcgtatccgt gcgcatcatg gcgcgcagca
    30001 taccacatca ctgcgaggag gcgggaggcg catctgcaag ggctgggttg accctttatg
    30061 aatgaatgcc cgcttgtgcg tcgcgaccga attcactccc ttgccgccct caactaggtc
    30121 aatacctcgt tgctgtcagc ggggagactc tctagagttc tttactaaga ttgcgaggtg
    30181 ctcatgaacg tcttgctggt gcatccaggc ccgctcatgt atacgaaaat atatctgcgt
    30241 ctcgaaccgc tcgggcttga gctcgttgcc tcagcggcac gggcactcgg acatcgtgtg
    30301 acattgctcg atctgcaagt ggaatcgtcc accgactatg tccgcctgct acgcgagcac
    30361 cgtcccgacg ccgtcggctt ttcatgcaat tacctggcga acgtgccgga agtcgtggac
    30421 ctggccaaac tcaccaaacg cgtagttccc cgctgtgcgg tgttcgtcgg cggtcatagt
    30481 gcgtcattcg tcgctgagac gattctcgtt catggcgacg gagcaatcga ttgcatctta
    30541 aagggcgaag gggagaccgc cgtcggcccc gtgctcgctg cgctcgctga tcgggacaat
    30601 cagccaggcg tgattccggg agcgatcacg agcgctggcg agggaccgcc ggtcggaatg
    30661 ctcgagcacc tcgacgctca tcggcccgcc cgcgacttgc tccgtcaccg cagaaaatac
    30721 tttatcggcg tcttggatcc ttgcgcctca atcgagttca cccgcggctg tccgtgggac
    30781 tgctcctttt gcagcgcgtg gaccttttac ggtcggagct atcgcgtccg tacacccgaa
    30841 gcaattgttg aagacctgaa gactgtcggt gagccgggcg tcttcatcgt cgatgacgtc
    30901 gcatttatcc aggccaaaca tggtttggcc attgccgagg ccatcgccag gcaaaagata
    30961 aaaaagcgtt attatctgga aacacgaggc gacgtgctct tgcgaaacaa agacgtcttc
    31021 cgtatgtgga agacccttgg actcgcctat atgtttatcg ggatcgaggc aatcgacgag
    31081 gaaggcctgg cactgcaccg gaaacgcatt acgctcagca agaattttga ggcgcttgaa
    31141 ttcgcgcggt ccctgggcat cacggtcgcc atcaatctca tcgccgatcc gtcatgggat
    31201 cgccgacgtt ttgagatcgt gcgtcagtgg tgtctcgaaa tcccggaaat cgtcaacatt
    31261 agcgtcaaca ctccctatcc gggcacggag acctggcata ccgaagccag gcacgtcacg
    31321 acacgggact accggctgta cgacattcag cacgccgtcc tccccactca cctgccacta
    31381 cctgagttct atcatgaact cgtgcagaca cagcagattc tcaatcgtaa gcacttgggt
    31441 tggaatgcct tgaagtcgac cgcccgtatc gccgcaggtc atttactccg agggcagacg
    31501 aatttcgtga agatgctgtg gaagttcaac agcgtctatg accctcgtct ccaactggca
    31561 gaccatgcgc gtccggtcct ctacgaaatg ccgctgcctc gaaggcagac gtccggcgtg
    31621 cgtacggact tactgtacat tcaccacgca cgaggccgcg ctggccgtgt cctggacgac
    31681 gccacggagc aattcgtcga aacgacgcgc atgggaaaga cggcctaaat gtctgccgcc
    31741 ctgccttcga gcgagacctc acatcttcac gagcgctgcc cggcctgccc ttgccacagc
    31801 aggtcataac ccaattcctt ggtctcagca cacatggagg cgagagcgga gaattttttc
    31861 gtgaacagct cgttcgcgtg agagcgctca tgatcctcga aggatttcca gtaggtgacg
    31921 atcacgtaat gatcttctgc agtcttggat gacgaaagtg agccttcttc cgagacaaac
    31981 ccggaaaatt tgaacacctg ccccgcgaga aacccgcctt tgtctcctcc gtaggtgtct
    32041 ttcacgacgc tgcacagctc gccgacagcc aattcgatgt cctcgaaggt gatgccgggt
    32101 ttcagcgtga ccgtattgaa caacattttg gctccgaatg gcactgtgat gggtccgaac
    32161 atggtaattc ctctcctctc cgtgagccgg ttaggcgtgc acgcgacgtt cgccgatgcc
    32221 gaggcgtact gcttcaatgt ccaattcatg ctccagccgg tggaggactt cgtcgctgat
    32281 cgtcccgtca ttccgcatgc cgatcaacgc catccgttcg gcagtaagcg tctcatggcg
    32341 gagccgccga aaggcctcgg ctgactccag cgaacattcc ggatccacgt ggccgttccc
    32401 tccaaaccgt tgcatccggc ggccataatg gtctcgcagc cgctcaacct gctctccgac
    32461 ggaccaggac tcgccagcca atgtgtccaa ccgtgtcagg gccatggagg ccgcatgttc
    32521 gcgcgcgagc atttcctcct gctcgaaccc tcgatcctct tcaagatgca aggcccggat
    32581 gaggggggtg agcgacagcc cttgcagcac cagtgtgacc aggatgaccg taaaactgat
    32641 caggatgatt tccgcccgga agggcagcgg tgtgccggct gcggtggcca ccggtaaggc
    32701 gagggccgca gcaagggtga cgatgccgcg catgcccgtc cagccgatca aaaagatatt
    32761 cggccaagca ggaagcggat cgcgtgcccg caacgacgca ctcaaccagc gtggaatcag
    32821 cgcagcgagc ggtacccagc caagacggac aacgatggcc gtgaggctga cgacgatgcc
    32881 ggtcacgagg accgggccta ggccgccggg ggggatcgcc tggctcaagg cacgaagttc
    32941 caacccgatg aggatgaaga tgaatccgtt caacacaaac acgagcacat cccagaccgc
    33001 ccggccttga atcctggtga tcggcgacac ggcggcactg aaatgctgtc ggaggtgcaa
    33061 gcctcccgcc acacaggcca gcacggccga cgcatgaacc tgttccccca cgacccacgc
    33121 tacgtatgga gcgaggagcg taatggcaat ttccgtaaag ctgtcatgag tcgcacacaa
    33181 cgcccaccgt gtggcgatgc tcacggcgat tcctatcgcg accccgacgg ttgccgcaaa
    33241 cataaacgtc acgagtcctt gtcccggaac gaaacttcct ccaacggcga cccccactgc
    33301 catgcgatag aggaccaacg cggtcgcatc gttgaccaga ctttcgcctt cgaggatcgt
    33361 aaccacccgt cgagggatgc gcagccgctt cccgatggcg gtggccgaga ccgcgtccgg
    33421 tggcgacacg atggcgccaa gcgcgatcgc ctccgcccat cccatgcctg gcaggagcca
    33481 gcgcgccacg accgccacag cgccgcttgt cacgactacc aacccgatcg cgagcaacga
    33541 aatcggccgg aggttgcgac gaaactcccg ccaggacgtg aaatatgcgg ccgcccacaa
    33601 aatcggcggg agaaagacca gaaagacgag atccggattc aaggtgaccg tcgggacgcc
    33661 aggtatgagg ctcaggatca atccgccgat gaccaggagg atgggatacg gcaccaccac
    33721 gatcttctgg gcgatcgtcg taagcgcgag cacgatgccc agcagaagaa tcacgatttc
    33781 gagctggtgc aatccttcca taaacgtctc gacagtccgc ttggagcgct gtgggtgagt
    33841 aggctacgtg gcaggcctcg aggagtcaat cgggtgccga gggggataga gtaccaactc
    33901 gatgaataga acaatgccgc aggcatcagc aataggctgc gaaggacggt caggcgatca
    33961 gcggaccggc ggtccgctgt ccggaatctg aagcgtaata gaaatcgccg gtccttcagg
    34021 cggaacctgc gccaaaatct tgtcccctgt cttcagtctc gcggacgcac cctggatctt
    34081 cgtgtcggcg gtcactcgaa tgcgtacctc ccggccggag atgtctttca cgaccagttc
    34141 ctctccttcc caatacaaga catcgccctt gacctccctg gcggcggtcg cctgagctgg
    34201 ttccgcaaat gccgagtcaa ccgtaccggg acacaacagc cacagcagca acaagaatgc
    34261 cataggctat cctcctccct ggtttcagcc tagaaatcct acccaccctt gtcagtgtac
    34321 tgggataaac ctctgtgcgc aatcgcgggt cagggaaccc tcgaagcaaa tctagggaaa
    34381 ggacgagagc ctcaatggca caaaaagagc gatcgttcag catgcagtct actgtgcaat
    34441 gcggcatcac ctgctggctt gtcctgagcg tcgccgtcct gccagctgca gcgcagtccg
    34501 atcgattcgg tggacccgta gaattccgtg ttcccgatcg gccgatcagc gtcccgacgc
    34561 tccgctttcc cgaagctgcg ggtctatcct cagacaagcc ctcgagtacc tccgggatgt
    34621 cagcccctga aaacgtactc acacacctcg ataaggaatt gggtcgaacg ctgagcagat
    34681 ccgaacccgg ccggcgaggt ccgccggcta cgaccgacga ccagcgtgaa gagcgggcgc
    34741 tggcgaaggg ctcgttcaag taaccgcctg tgtacaggct ccgacatctg ggtggtctcc
    34801 actcgcgaca cgcgcgggct gtttgagctg gctgatcgcc tcgatcccct ggttctcggt
    34861 gaacgacgcc agggcagtag gcggg
//
LOCUS       DASSCQ010000097        13587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_693839, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000097 DASSCQ010000000
VERSION     DASSCQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693839"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFH05_06800"
     CDS             complement(<1..1193)
                     /locus_tag="VFH05_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677956.1"
                     /translation="MASFDREDLQQALAPLCTDVPPDVLRDFVTRMDDDYLRRVPLQT
                     IAQHVRLAARLTPEHLCECTVFDRGDGHFDLTVVAYDYFAEFATICGLLSAFGLNIEE
                     GQIYTFTETPVFSPTTKPAYGEGRSQRPKLRPGLSRKKIVDLFRIQPVGGASFTPENQ
                     DRFTDELRTLIGLLDDSKFEQARHAVNRQLVEQLGKQRGSFSGLLHPVQISFDNSQSP
                     SDTIMDIRSDDTPAFLYAFANALAMRNVYIARAQITLESGKLHDRFAVRNRYGRKFTD
                     PVDQQQLRLTAVLIKQFTHALTWAPDPTKALEAFDQFLDLTVQETKGKAREEALAFLG
                     DKKTFPLLARLLGASDFLWEDFLRRQHDHLLPLLQDYRDAPLIKPQTVLRKALDRVVE
                     RHKDEA"
     gene            1502..3193
                     /locus_tag="VFH05_06805"
     CDS             1502..3193
                     /locus_tag="VFH05_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677957.1"
                     /translation="MEPPIGKPDPTPLLLASERTPQDIRGILSAYGLTDVEQADRNLQ
                     AMAGQPHERHQLVGILPILMESVARTADPDQALNHWERLLENVTRSTFLDYLRSSPRM
                     LELLSTIFGNSDSLAFTIIRDPTLVYWLAEEDVLSRPSTRSGLEQALRKQLARLTVKE
                     LKLAVLRRFRRREMLRIGVRDLLRLAKVPETTASLSDLAALLIEAAYGIVDADLGRQY
                     GKPMHKNRQGRWVETGFAIVGMGKLGGHELNYSSDVDLIYVYASHEGETRAASARAVK
                     PMAAGISNEEYFEILSRELTKALSEQTREGYVFRVDLRLRAEGSVGQLARSVEDYRKY
                     YFTRGRIWERLALLKAWPVAGSPEVGKAFLRAVRPFILGQGTSKSGLAGAMAVVEDVR
                     TVKELIDAKMADRGHEHRNVKLGTGGIREIEFLAQTIQVLAGKQHPALLHRSTLDSLK
                     AFTRKQLLSTKERDALSAAYLFLRDIEHKLQMVHDLQTHSLPDEALELERCAIRSGYQ
                     GTNRPVIASRFQADYRSHTKVVHDLFRSFFYETGASPLLKATLKAMGIRRPSQGA"
     gene            complement(3181..3537)
                     /locus_tag="VFH05_06810"
     CDS             complement(3181..3537)
                     /locus_tag="VFH05_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677958.1"
                     /translation="MIVEVEANENCEASLFARFKEAGPPRQVDQVRLYDRNPSGEWYW
                     VTGWTDNAEAPVSPAYAQLVEDSGSGLAHLVYGGIYGLRFKPVVVNEPWSLDSANQWG
                     ETHLSLATDRDLRYAP"
     gene            complement(3659..5302)
                     /gene="groL"
                     /locus_tag="VFH05_06815"
     CDS             complement(3659..5302)
                     /gene="groL"
                     /locus_tag="VFH05_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6677959.1"
                     /translation="MPKQLLYSEAARAAILKGVNQLADAVKATLGPKGRNAILDKKFG
                     APTITKDGVTVAKEIELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIFREG
                     AKNITAGANPMEIKRGIDKAVEAVVAELKKLSKPCQNKTEISQVGTISANNDKTIGDL
                     IAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMEASLEEPL
                     ILINEKKISSMKDLLPVLEQVAKMGKPLVIIAEEVEGEALATLVVNKLRGTLNVAAVK
                     APGFGDRRKAMLEDIAILTGGQTISEDLGIKLENVKLTDLGRAKRVTIDKDNSTIVEG
                     YGDPKKIEARVKQIKAQIEETTSDYDREKLQERLAKIVGGVAVINVGAATETEMKEKK
                     ARVEDALHATKAAVEEGIVPGGGTAYLRCIKALDTIKDVPAEQKVGLDIIRRALEEPI
                     RQIVNNAGGEASVVVGKVREDKNVNAGYNAATEEYVDMIKAGIIDPTKVSRTALQNAA
                     SVAALMLTTEVMVTDIPEEKKEPMGGGGHGHNHGMEGMY"
     gene            complement(5334..5633)
                     /locus_tag="VFH05_06820"
     CDS             complement(5334..5633)
                     /locus_tag="VFH05_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET6677960.1"
                     /translation="MSTAAKEKKNVAKGFQPLGDRVFVTYTEELERTSGGIYVPDSAK
                     EKPQRGIVQAVGKKVENVKVGEQVLFDKYSGSKLRIEDEECLILKEEDILGIFTS"
     gene            complement(5894..6886)
                     /locus_tag="VFH05_06825"
     CDS             complement(5894..6886)
                     /locus_tag="VFH05_06825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HET6677961.1"
                     /translation="MAKRQAKKQQEEEILEPEVLGPDDAMPEGDTPSTELPPSADGRT
                     DTTAVVPVTALQQYLAEIRRHPYLSKEEELQLFEEYRTQGSREAAVKLIMANLRVSVA
                     IAAEYLHTGADHMDLIQEGNVGLLQAIKKFDPSKNVRFHAYAAWWSRAYILRYLLNTY
                     RLVKIGTTQDQRKLFYNLKKEKSKLEREGFSPDTKLLADRLNVRERDVIEMDQRLGNW
                     ELSLDQPLGEDQDGSLLDILPSQQVPADEVLARDQLRTLFREKLGEFIKTLDERDEDI
                     LRNRLLSETPLTLDDLGAKYGITKERTRQLEARIIKRLRDYMKKDIKDFDQLRA"
     gene            7061..8260
                     /locus_tag="VFH05_06830"
     CDS             7061..8260
                     /locus_tag="VFH05_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6677962.1"
                     /translation="MHPLTVLKSGHWPSLLGAWLHLTISFMVWLLIGAMSISIAGDLQ
                     LSNSQISLLVALPLLGGAVLRMVAGWSADRIGARSTALVIMLTECAVLLWGWLGVKSY
                     AEALAFALCLGIAGASFAVSLPLASRAYSPAAQGLVLGLAASGNVGTVFILFWAPRWA
                     EQLGWHAVCLVMAGVVAMSLLLFSVVVPGDPDRPVHQDARWWHSVASMIRRRASYWLC
                     FLYATTFGGFVGLCSLLPLVLHDVYRFEAITAGVLAAVCGLGGSLLRPVGGYVADRMG
                     GLRTLHYVLPIAAGAVVAIISPSLSLAVTMMILTTGALGFGNGVVFQLVAEWFPKDIG
                     LASGVVGAAGGLGGFVLPVAAGLLKDTAGSYEYGLWLFAGFVMCAWGTVIAALRKSHE
                     AAPGSSS"
     gene            8372..9580
                     /locus_tag="VFH05_06835"
     CDS             8372..9580
                     /locus_tag="VFH05_06835"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET6677963.1"
                     /translation="MQPPFDEIEDAIRDIKKGKFVILVDDEDRENEGDLVIAAEKVTP
                     QAINFMARHARGLICLALTPERVEELQLPQQAVENTAAFGTAFTVSIDARKDITTGIS
                     AADRATTIHVAIDPKSKPSDLARPGHIFPLKAQRGGVLRRAGQTEGSVDLARLAGLLP
                     AGVICEIMNEDGTMARVPELTKFAKTHKLKMVTVKALIEYRMRRETFIKRMASARLPT
                     IFGEFDAVAFENEIDHITHIALVKGRVDNGAPTLVRVHSGCLTADALGSLRCDCRDQL
                     HAAMEVIQKEGRGVLLYLNQEGRGIGLLNKIRAYGLQDKGSDTVEANLKLGFKADLRD
                     YGVGAQILANLGLHQIKLITNNPRKIVGIEGYGLKVVERVPIEIQPHAGNVKYLKTKK
                     KKLGHILKKV"
     gene            9625..10170
                     /gene="ribH"
                     /locus_tag="VFH05_06840"
     CDS             9625..10170
                     /gene="ribH"
                     /locus_tag="VFH05_06840"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET6677964.1"
                     /translation="MKLRKGSHNATGLKFGIVVAKFNKFVTSKLLSSCIDGLTKHGVV
                     PEDIEVVRVPGAFEIPLVARTLAGTKRFDAVICLGAVIRGDTPHFDYISAEASRGIGQ
                     AALDADVPIIFGVLTTDTVAQAIVRAEPTKFNRGGEAAKSAIEMATVMRMIRGGEATA
                     LPKETAGTNGAARRPARKRKT"
     gene            10173..10631
                     /gene="nusB"
                     /locus_tag="VFH05_06845"
     CDS             10173..10631
                     /gene="nusB"
                     /locus_tag="VFH05_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6677965.1"
                     /translation="MGSRHQSRERALQILFQYDIHGKPGVWLDEFWNQCHATEDVRSF
                     AEQLVTGVLGHKNDLDALIGKYATNWKVSRMQIVDRNILRLGAYELLWLDEVPAKVTV
                     NEAIELAKDFGDEEAAKFVNGILDKVLTTEAKLALKRQEAVRQPQSGREE"
     gene            10647..11975
                     /gene="purB"
                     /locus_tag="VFH05_06850"
     CDS             10647..11975
                     /gene="purB"
                     /locus_tag="VFH05_06850"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET6677966.1"
                     /translation="MIDRYTRPQMKAIWDLKHKYEIWLEVELQACAAFEKAGQAPRGT
                     SAKIRNKARIDVSRIAEIEKVTKHDVIAFLESLADTVGPEHRFLHMGLTSSDIVDTSL
                     AVQMTEALDLILEGVQGLLAVLKRQAFRYKSQVMVGRSHGIHGEPISFGFKLAIWHEE
                     VGRHLARLRQVRREIAVGKLSGAMGTFAHQGPDIEAYVCAKLGLTVDPVSNQVVQRDR
                     HAAYATALALLAATIEKIATEIRHLQRTEVLEAEEYFSAGQKGSSAMPHKRNPIVSEN
                     LCGLARLVRANSLAAMENVALWHERDISHSSVERVIMPDSTILIDYMLAKVTDLIKHL
                     VVYPANMKRNLELTGGLVYSQRLLLALIDKGAQRKESYEAVQRNAMASWNGSGNLQEL
                     VAKDPFIAQHLKKSEIAACFNPKYYLRHVDHIFKRVFKGVQHSPSRKGNA"
     gene            11972..12766
                     /locus_tag="VFH05_06855"
     CDS             11972..12766
                     /locus_tag="VFH05_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET6677967.1"
                     /translation="MTAQRATLLAIIVIAASMWSDLGFAAADREKLLGDPGVYGTFAA
                     YTLNEDWAKRDGPTRIALLSVLKGVVEQHREKIAIDLYLLRGLSDHADILFRAHANEL
                     RDIQKFLLDLQASPFGKHLTVAGIMHGLTKKANYVPGFPDDIKADLKTASDPGPKPYA
                     AVIPIRKSADWWAMDQDSRAAMMKEHTQASLPYHKTVKRKLYHSTGLDDIDFITYFET
                     SKLEDFHGLILAQERVKEFQYVRRFGHPTWLGTVKSLDEIIEILAQ"
     gene            12793..13497
                     /gene="purC"
                     /locus_tag="VFH05_06860"
     CDS             12793..13497
                     /gene="purC"
                     /locus_tag="VFH05_06860"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET6677968.1"
                     /translation="MVTGTLIYEGKAKKLFTTGRSDEVLQYFKDDATAFNAQKRGTIL
                     DKGIINNKVSERLFRLLEESGVPTHFVERKSDREMLTKKVTIVPVEVVVRNVIAGSLA
                     KRLGLKEGEAIQPPIVEWYYKNDGLGDPLIADEHVRLLKLATPEQMAEIKGLALKVNS
                     VLQPFFAERRMVLVDFKLEFGVHNGRLILADEISPDTCRFWDQATRESMDKDRFRKDL
                     GKIEEAYQEVLKRVCG"
BASE COUNT         2786 a         3842 c         4066 g         2893 t
ORIGIN      
        1 gcttcatctt tgtgccgttc caccacacgg tcgagcgctt tccgtagaac cgtctgcggt
       61 ttgatgagcg gcgcatcgcg ataatcctga agcaggggca gcagatgatc atgctgccgg
      121 cggagaaaat cctcccacag aaaatcgctg gcgcccaaca ggcgggcgag gagaggaaac
      181 gttttcttgt cgccgagaaa ggcgagcgct tcctctctcg cctttccttt cgtctcttga
      241 acggtgaggt cgagaaactg gtcgaacgct tccaaggctt ttgtggggtc cggagcccag
      301 gtcagggcat gcgtgaattg cttgatcaag actgcggtca ggcgcaactg ctgctggtcc
      361 accgggtcgg tgaacttccg cccgtagcgg ttcctgacgg caaagcggtc atgcagcttg
      421 ccgctttcca gcgtaatctg tgccctggcg atatacacat tccgcatggc gagtgcgttc
      481 gcgaaggcat acagaaaagc cggcgtgtcg tccgaccgga tgtccataat ggtgtcgctc
      541 ggcgattggc tgttatcgaa tgaaatttga accggatgga gcagaccgct aaaggagccg
      601 cgctgtttgc cgagctgttc aaccagttgc cgattcaccg catgccgggc ttgttcgaac
      661 ttgctgtcat cgagcaagcc gatcaaggtc cggagttcgt ctgtaaaacg atcctggttc
      721 tcgggggtga acgacgcgcc gccgaccggt tgaattcgaa aaaggtccac gatctttttc
      781 ctgctcaaac cggggcgcag cttaggccgc tggctccgtc cttctccgta tgcaggcttc
      841 gttgttgggc tgaaaactgg cgtctcggtg aacgtataga tctgcccttc ctcgatgttc
      901 aacccgaatg ccgacagcaa tccgcagatg gtcgcgaatt cggcaaaata gtcataggcg
      961 acgacagtca gatcgaagtg tccgtcacca cgatcgaaga ccgtgcattc gcaaagatgc
     1021 tccggggtta atctcgctgc caaccggacg tgctgcgcga tcgtctgcag cggaacccgg
     1081 cggagatagt catcgtccat tcgggtgacg aaatcacgaa gcacatccgg gggcacgtcc
     1141 gtacaaagag gggcgagagc ttgctggagg tcttcgcggt cgaaggaggc catgggggcg
     1201 tcagtatatc gcaggaccgt tcattgtgcg caatctgggc tgacagtata atgccgcgcc
     1261 ggcaggctgg tgaaacagga cgacagattt cgttctcgcg gcctcgacat atgcaccagg
     1321 aaggcgctgc tccgcaggtc ggggtcggca ggtcagggaa ggaggagcag ctcggggctg
     1381 gacgggcatt ctccgggacg ttgacattca tcaaccatgt agtgctgagg cggtcttggg
     1441 gactaaaaag gaagaacggc cactaagccg gagagcacct tccaagtcgg cacaggtgtc
     1501 gatggagccg ccgattggca agccggatcc cacgccgttg ttgctggcat ctgaacgcac
     1561 tccgcaggac atacgcggca tcctgtcggc ctacgggctc acggatgtcg aacaggccga
     1621 ccgcaatctg caggcgatgg ccggccagcc gcatgagcgg catcaactcg tcggcattct
     1681 gccgatcttg atggagtcgg ttgcgcgcac cgccgacccg gaccaggcat tgaatcattg
     1741 ggagcggctt ctcgagaacg tgacccgctc gacctttctc gattatctgc gcagctctcc
     1801 gcgcatgctc gagctgctct ccaccatttt cggcaatagc gattcactcg ccttcaccat
     1861 tattcgcgac cctacgctgg tgtactggct ggcggaagaa gatgtcctct cgaggccgtc
     1921 gacgcggtca ggcttggagc aagcgcttcg gaagcaattg gcccgcctca ccgtgaaaga
     1981 actgaagttg gccgtgttgc gccgctttcg acgacgcgag atgcttcgaa tcggggtgcg
     2041 ggacctgttg cggctggcga aggtgccgga gacaacggcc tcactgtcgg atctggccgc
     2101 gctgctgatc gaggccgcct atggcattgt tgatgccgat ttaggccggc agtacgggaa
     2161 acccatgcac aagaaccggc agggcagatg ggtcgagaca ggctttgcta tcgtggggat
     2221 gggcaagctc ggcgggcacg agctgaacta cagttctgac gtggatttga tctatgtgta
     2281 cgcctcgcat gaaggggaga ccagggccgc atcggcaaga gccgtcaaac cgatggctgc
     2341 cggcatctcc aacgaagagt atttcgaaat tctctcgcgc gagctcacca aggcattgtc
     2401 cgagcagacc cgggagggat acgtcttccg cgtggacctg cgcttgcgtg cggaagggtc
     2461 ggtcggacaa ctggcccgat cggtggaaga ttatcggaaa tactatttca ccagggggcg
     2521 gatctgggag cgacttgccc tattgaaagc ctggccggta gccggctctc cggaagtggg
     2581 gaaagccttt ttacgagcgg tcaggccgtt tattttgggt cagggcacca gcaaatccgg
     2641 tctggcaggc gccatggccg tcgtcgagga tgtccggacg gtcaaggaac tgatcgatgc
     2701 gaagatggct gatcgggggc acgagcatcg caacgtcaag ctcggcaccg gtggcattcg
     2761 ggaaatcgag ttcctcgcgc aaacgatcca ggtgctggct ggcaagcagc acccggcatt
     2821 gctgcaccgc agcaccctcg attcgttgaa ggcgtttacg cgaaaacagc ttctgtcgac
     2881 gaaggagcgc gacgcgctgt ctgccgccta tctgtttctt cgagacatcg agcacaaatt
     2941 gcagatggtc catgatctgc agacgcactc cctaccggat gaggccctgg agcttgaacg
     3001 ttgcgccatc cgttcgggat atcaggggac caatcgtcca gtcatcgcca gcaggtttca
     3061 agcggactat cgttcgcaca cgaaggtcgt ccatgatctg ttccggtcgt ttttctacga
     3121 gaccggagcg tcgccattgc tcaaggccac gctgaaggcg atgggtatcc gccggccgtc
     3181 tcagggggca tagcgcaaat cacgatctgt tgccaacgac agatgcgtct ctccccattg
     3241 gttcgcacta tcgaggctcc acggctcatt gacaacaaca ggcttgaaac ggagaccata
     3301 gatcccccca tagacaagat gcgccaaccc tgagcctgaa tcttcgacca gttgtgcgta
     3361 ggcgggggag acgggcgcct cggcgttgtc agtccagccg gtgacccaat accactcgcc
     3421 cgaaggattc cgatcgtaga gccgcacctg atcgacttgc cgtggtgggc cggcctcttt
     3481 gaaccgagcg aacaggctag cttcacaatt ctcgttggct tcgacttcga cgatcatgct
     3541 cgccgttcca tggattcgtt cattctagtg aatcagtctc aacaaagaaa aagggcccgg
     3601 cggttctccg ccgggccctt cattcgaccg cagctatcgg acgatagccg gtagcctctt
     3661 agtacattcc ttccatgccg tggttgtggc catgcccgcc gccgcccatg ggttccttct
     3721 tctcttcagg gatgtcggtg accatgactt cggtcgtcag catcaacgcc gccactgacg
     3781 ccgcgttctg caacgcagtt cgcgacactt tggtcggatc gatgataccg gccttgatca
     3841 tgtcgacata ctcttcggtc gccgcattgt agccggcgtt cacgttcttg tcctcgcgga
     3901 ccttgccgac cacgaccgag gcttcaccgc cggcattgtt cacgatctgg cggatcggct
     3961 cttcgagcgc gcgtcggatg atgtccaagc cgaccttctg ctccgccgga acatccttga
     4021 tggtatccag cgccttgatg cagcgcagat aagccgttcc gccgcccgga acgatgccct
     4081 cttccaccgc cgccttggtc gcgtggaggg cgtcctcgac tcgggctttc ttttccttca
     4141 tttccgtctc cgtcgcggcg ccgacattga tcaccgccac accgccgacg atcttggcca
     4201 gccgctcttg cagcttctcg cgatcgtagt cggaggtcgt ttcctcgatc tgggccttga
     4261 tctgcttcac acgggcttcg attttcttcg ggtcgccata gccttcgacg atcgtgctgt
     4321 tgtccttgtc gatcgtcacg cgcttggcgc ggccgaggtc ggtgagcttc acgttttcca
     4381 gcttgatgcc gaggtcttcc gagatggtct ggccgccggt gagaatcgcg atgtcctcca
     4441 gcatggcctt ccgtcgatcg ccgaagccgg gcgctttcac ggcggcgacg ttcagggtgc
     4501 cgcgcagctt attgaccacg agggtggcca acgcttcgcc ttcgacttct tccgcgatga
     4561 tcacgagcgg cttgcccatc ttggcgacct gctcgaggac cgggaggagg tccttcatgc
     4621 tgctgatttt cttttcgttg atgaggatga gcggctcttc gagtgaagct tccatccgct
     4681 cggcgttggt gacgaaataa ggggagatgt atccgcgatc gaactgcatg ccttcgacga
     4741 catcgagtga ggtcgtcatg gacttggcct cttcgaccgt gatcacgcca tccttgccga
     4801 ccttttccat cgcttccgcg atcagatcgc cgatggtctt gtcgttgttc gctgaaatgg
     4861 ttccgacttg tgaaatttcg gtcttgttct ggcagggctt gctgagcttc ttcaactcgg
     4921 ccaccaccgc ttcaaccgcc ttgtcgatcc cgcgcttgat ttccatcggg ttcgcgcctg
     4981 cggtgatgtt cttcgcgcct tcgcggaaga tggcctgcgc caggacggtg gctgtcgtgg
     5041 tcccgtcacc cgccgtgtcg ctggtcttgc tggcgacttc tcggacgagc tgagcaccca
     5101 tgttctcgta cggattcttg agctcgattt ccttggcgac ggtcacgccg tccttggtaa
     5161 tggtcggggc gccgaacttc ttgtcgagaa tggcgttgcg gcccttgggt ccgagcgttg
     5221 ccttgacggc gtcggcgagc tggttgaccc ccttgagaat ggcggcccgc gccgcctcgc
     5281 tatagagtaa ttgctttggc atgtgatccc tccgtaaaag tctgtttgtc gaattagctg
     5341 gtgaagatac cgaggatgtc ttcttccttg aggatcaagc actcctcgtc ctcgattcga
     5401 agcttgctgc cagagtactt gtcgaacagg acctgctcgc cgaccttgac gttttcgacc
     5461 ttcttgccga ccgcctggac gatgcctcgc tgcggctttt ccttcgccga atcgggcaca
     5521 tagatgcctc cggacgtccg ttccaattcc tcagtgtagg tcacgaacac gcggtcgccg
     5581 aggggctgaa agcccttggc cacgttcttc ttctccttcg ctgcggtgct cataacagaa
     5641 cctcctcgtg atgaaaagtt aacccgacca atgtcgacga tttagaacgg gttcgcactc
     5701 gatggcacgg gctcaagagc ccccgcgagt agaatccctg atctgccgag actgcgggat
     5761 agatagaact gtcttggtct gagtcaaggg gcctagggaa aaatcttttg atccctcggc
     5821 tcaggaaggg ggcatcataa ccgaggacgc tccggatatt ccactgaatt ttgtgggatg
     5881 cgggaagatg ggatcaggct cgtaactggt caaaatcctt tatatccttc ttcatgtagt
     5941 cccgcagccg cttgataatc ctggcctcca actggcgggt gcgctccttg gtaatgccat
     6001 atttggcgcc caggtcgtcg agcgtgaggg gtgtctcaga caagagacgg ttgcgaagga
     6061 tgtcttcatc gcgctcatcg agggtcttga tgaattcgcc cagcttttct cgaaagagcg
     6121 tccgcagttg atcgcgagcc agtacctcgt ccgccgggac ctgctgagac ggcagaatat
     6181 caaggaggct gccgtcttga tcctctccca gtggttgatc cagcgacaac tcccaattac
     6241 ccagccgctg gtccatctcg atcacgtcac gctcacgcac gttcaaccgg tccgccagca
     6301 atttggtgtc cggcgaaaag ccctcgcgct cgagcttcga tttctccttc ttgagattgt
     6361 agaacagttt tcgctgatcc tgggtggtcc cgattttgac cagccgatag gtattgagaa
     6421 gatagcggag aatgtaggcg cgcgaccacc aggcggcata cgcgtggaac cggacattct
     6481 ttgaggggtc aaacttcttg atcgcctgca gtaagccgac attgccttcc tggatcaaat
     6541 ccatgtggtc ggcgccggta tgcagatatt ccgcggcaat cgccaccgag acgcgtaaat
     6601 tcgccatgat caatttcacg gccgcttcac ggctgccctg cgtgcgatat tcctcaaaaa
     6661 gctggagttc ttcttccttc gagaggtagg gatggcgacg tatctcggcc aggtactgct
     6721 gcagtgcggt gacgggcacc accgccgtcg tatcggtacg cccgtcggcg gacggaggca
     6781 attcagtgga cggcgtgtcg ccctcaggca tggcgtcgtc cggaccaagc acctccggct
     6841 cgaggatttc ttcttcttgt tgtttcttcg cctgacgttt tgccatacga agcgcaaact
     6901 atagcagaac gtcctgccgc gacaaagctg ccggtgtccc taaggttcct tgcagggatg
     6961 aaattcgtcc actatgatgc ggagttctgt cggagcgccg gcccggatac cgcgaacctc
     7021 aggagggcgg gcgcgacttc gaccaccagg aactcgagcc atgcaccccc tgaccgttct
     7081 gaagagtgga cattggccct ccctcctggg tgcctggctg cacctgacca tcagtttcat
     7141 ggtctggctg ttgatcggcg cgatgagcat ttccattgcc ggcgatcttc agttgagcaa
     7201 ctcgcagatt tccttgctcg tcgcgctgcc cttgctgggc ggcgcagtgc tcagaatggt
     7261 cgcgggttgg agcgcggatc ggatcggcgc acgcagcacg gcattggtca tcatgctcac
     7321 agaatgcgct gtgctgctgt gggggtggct tggagtgaag tcatatgccg aggcgctggc
     7381 cttcgcgcta tgcctgggga tagctggggc gagctttgcc gtctccttgc cgctcgcaag
     7441 cagggcctat tccccggccg cccagggact cgtactgggc ctggcggcat ccgggaatgt
     7501 cggcaccgtc ttcatcttat tctgggcgcc tcgctgggcc gaacaactag gctggcacgc
     7561 tgtctgtctc gtcatggccg gtgtggttgc gatgtcgctc ctgcttttca gtgttgtggt
     7621 gcctggggat cctgaccgac cagtacatca ggacgcgcgt tggtggcaca gcgtggcctc
     7681 gatgatccgg cgccgggcct cctattggct gtgtttcctg tacgcaacga cattcggcgg
     7741 gtttgttggt ctctgcagtc tgctgcctct cgtccttcac gatgtgtacc gcttcgaagc
     7801 catcactgcc ggagttctcg ctgcggtgtg cgggctggga ggaagcctcc tccgtccggt
     7861 cggcgggtat gttgcggatc gcatgggcgg cctccgcacg ctgcactatg tcttgcccat
     7921 tgcggccggg gccgtcgtcg ccattatcag tccctccctt tcgctggctg tgacgatgat
     7981 gattctcaca accggcgcac tgggatttgg caacggcgtc gtgtttcaac tcgtcgccga
     8041 gtggtttccg aaggacatcg gactggcctc cggagtggtc ggcgcggccg gtgggctcgg
     8101 cgggttcgtc ttgccggttg ccgcgggcct cttgaaggac actgccggaa gctatgaata
     8161 cggactctgg ctcttcgccg ggtttgtcat gtgtgcctgg ggcaccgtga tcgctgcatt
     8221 gcgcaaaagt catgaggcag cccctggctc ttcgtcctaa aacgcgtgga ctcgaggcaa
     8281 gcggcttaaa aaaacccatg ttaatggtac atgttcaatg ataaattgat cgtccttaac
     8341 gatcggaaaa ggccatgcct gtcaattgag tatgcaaccc ccttttgacg agatcgaaga
     8401 cgcaatcagg gacatcaaaa agggcaagtt cgtcatcctg gtcgacgatg aagaccggga
     8461 gaacgaaggc gatcttgtca ttgccgcaga aaaagtcacg cctcaggcca tcaattttat
     8521 ggccaggcat gcgcgcggac tgatctgcct cgcgctgacg cctgagcgcg tcgaagaatt
     8581 gcagttgcca cagcaagccg ttgaaaatac cgcggcattc ggcaccgcct tcactgtctc
     8641 gatcgacgct cgcaaggaca tcacgaccgg tatttctgcc gcagaccgtg ccacgaccat
     8701 ccatgtggcc attgatccca agtcgaaacc cagcgacctc gcccggccgg gccatatttt
     8761 cccattgaag gcccaacggg gcggtgtgtt gcgacgggca gggcaaacgg aagggtccgt
     8821 cgatctcgca cggttggccg gtctcctgcc cgccggcgtc atctgcgaga tcatgaatga
     8881 ggacggcaca atggcacgcg tgcctgagct gaccaagttc gccaagacac acaagctcaa
     8941 gatggtcacg gtcaaggcac tgatcgaata ccggatgcgc cgggaaacct ttatcaagcg
     9001 catggcgagc gccaggctgc cgacgatctt cggggaattc gacgccgtcg cctttgaaaa
     9061 tgagattgat cacatcacgc acattgcgct ggtcaagggg cgggtggata acggagcccc
     9121 gacgctggtc cgggtgcact ccggctgtct caccgccgac gctctgggat ctctgcggtg
     9181 cgattgccgc gaccaactgc acgcggctat ggaagtcatc cagaaggaag gccggggggt
     9241 gcttctgtac ctgaaccagg agggacgggg aatcggactg ttgaacaaaa tcagggcata
     9301 cggacttcaa gataagggga gcgacacggt ggaggccaac ctcaagctgg gcttcaaggc
     9361 cgatctgcgc gattacggcg ttggcgcaca gatccttgca aatctcggcc tccatcaaat
     9421 caaattgatt accaacaacc cgcgcaagat tgtcggaatt gaaggatatg gactgaaagt
     9481 cgttgaacga gtgccgattg aaattcagcc gcatgcgggc aatgtcaaat atctcaagac
     9541 gaaaaagaag aagttggggc acattctcaa gaaggtctga gtcgggcggt tggaaagtgc
     9601 agaggcacaa caagctagca gattatgaaa ctccgtaaag gctcacacaa tgcgactggg
     9661 ctgaagttcg ggatcgtcgt cgcgaaattc aataaattcg tcacaagcaa gctgttgagc
     9721 agttgtattg atgggctgac caaacacggc gtggtgcccg aagacatcga agtcgtgcgt
     9781 gtgccgggcg cgtttgaaat cccgcttgtc gcgaggactc tagcgggaac caaacggttc
     9841 gacgccgtga tctgcctcgg tgccgtcatt cgaggcgata cgcctcattt cgactatatc
     9901 agtgcagagg ccagccgcgg catcggtcaa gcagccttgg atgcggacgt gccgattatt
     9961 tttggtgtgc tgacgaccga cacggtggcc caggccatcg tgcgagcgga gccaaccaag
    10021 ttcaatcgcg gcggcgaagc ggcgaagtcg gcgattgaga tggcgaccgt catgcggatg
    10081 atacggggcg gcgaggcaac agccctgcca aaggagaccg ccggaacaaa cggcgcagcc
    10141 agacgtccgg cacgaaagcg caagacctga ttatgggatc tcgccatcag tcgcgcgagc
    10201 gcgcgctgca aatcttgttt caatacgaca tccatggcaa acccggcgtc tggctggacg
    10261 agttctggaa tcagtgtcac gccacggaag acgtcagatc gttcgccgaa caactcgtca
    10321 ccggcgtgct cgggcacaag aacgatctgg atgcgctcat cggcaaatac gccacgaatt
    10381 ggaaggtcag ccgcatgcag atcgtcgacc gcaatatcct ccgcctcggc gcctatgaac
    10441 tgttgtggct ggatgaggtg ccggcaaaag tcaccgtcaa tgaagcgatc gagctggcga
    10501 aggatttcgg cgatgaggaa gccgcaaaat ttgtgaacgg cattttggac aaggtgctga
    10561 caacggaggc gaagttggca ctcaagaggc aagaggccgt tcgtcagcct cagagtgggc
    10621 gtgaggagtg agcgcccgga cgtccaatga ttgaccgcta cacgcgtcca cagatgaaag
    10681 ccatctggga tctcaaacac aagtatgaga tctggctgga ggtcgaactg caagcctgtg
    10741 cagcattcga aaaggccggg caggcgcccc gcggcacgtc cgcaaaaatc aggaacaagg
    10801 ccaggatcga cgtgtcgcgg atcgccgaaa tcgagaaagt cacgaagcac gacgtgatcg
    10861 cattcctcga atccctggcg gacaccgtag gaccggagca tcggtttttg cacatgggat
    10921 tgacctcatc cgatatcgtc gatacgtcgc tggccgtcca gatgaccgaa gcccttgatc
    10981 tcattttgga aggtgtgcag gggttgctgg cggtgttgaa gcggcaggct tttcgctaca
    11041 agagccaggt gatggtgggc cgttcacatg gtatccatgg cgagcccatc tcgttcggat
    11101 tcaagctggc catatggcac gaggaagtcg gccgacatct ggcacgcttg cgtcaggtgc
    11161 gccgggagat cgcggtcggc aagctgtccg gcgcgatggg gacgtttgcg catcagggac
    11221 cggatatcga agcctacgtg tgcgcgaagc tcggcctcac cgtggatccg gtctccaacc
    11281 aagtcgtgca acgggaccgt catgctgcct atgcgacagc tctggcgctg cttgcggcca
    11341 caatcgaaaa gatcgcgacc gagatccggc atcttcaacg gactgaagtg ctcgaggcgg
    11401 aagaatattt ttcggcagga caaaagggct cgtcagccat gccgcataag cgcaacccga
    11461 tcgtatcgga aaatctctgc ggattggcgc ggcttgtgcg ggccaatagt ctcgcggcca
    11521 tggaaaacgt ggccctgtgg cacgagcgtg acatcagcca ttcctctgtc gagcgcgtca
    11581 tcatgccgga cagcaccatc ctcattgact acatgctggc gaaggtcacc gacctcatca
    11641 agcatctggt ggtctatccg gccaacatga agcgcaatct cgagctgacc ggcggattgg
    11701 tgtactcaca acgcctactg ctggcgctca tcgacaaggg tgcgcagcga aaggaatcgt
    11761 atgaagccgt gcagcgtaac gccatggcct catggaacgg atcgggaaac ctgcaggaac
    11821 tggtcgccaa ggaccccttc atcgcacaac atctgaagaa aagcgaaatc gccgcctgct
    11881 tcaaccccaa atactatctg cggcacgttg atcatatttt caagagggtg ttcaagggcg
    11941 tccaacactc accttcacgg aaaggaaatg catgacagca caacgcgcga ctcttctggc
    12001 gatcatcgtc atcgcggcgt cgatgtggag tgatcttggc tttgcggcag cagaccggga
    12061 aaagctcctg ggcgatcccg gagtgtacgg gacgtttgcc gcctacacct tgaacgagga
    12121 ctgggcaaaa agggatggcc ccacgcgcat tgcgctccta agcgtcctga agggcgttgt
    12181 ggagcagcat cgagagaaga ttgccatcga tctctatctc ttgcgtggac tcagcgacca
    12241 tgcggatatc ctctttcggg cccatgccaa cgaactgcgc gacatccaga aatttctgct
    12301 tgaccttcag gccagcccgt tcgggaagca cctgaccgtt gcgggaatca tgcacggatt
    12361 gacgaaaaaa gccaattacg tgccgggctt tcccgatgac atcaaggccg atctcaaaac
    12421 ggcaagcgac cccgggccca agccgtacgc ggcggtgatt ccgataagaa agagcgccga
    12481 ctggtgggcg atggaccaag acagccgggc ggctatgatg aaggagcaca cccaggcctc
    12541 tttgccttat cataaaacgg tgaagcgcaa gctgtaccat tccaccggac tagacgacat
    12601 cgatttcatc acctatttcg aaacatcgaa gctggaagac tttcacggct tgattctggc
    12661 gcaggaaaga gtaaaggagt tccaatacgt gaggcgattc gggcatccga cctggctcgg
    12721 cacggtcaaa agcctggatg agatcattga aattctggcg cagtaagacg ctatagacgc
    12781 tggggagttc ttatggttac cggcaccctc atctacgaag gcaaggcaaa aaaactgttt
    12841 accaccgggc gttccgatga agtgcttcaa tactttaaag atgacgcaac ggcgttcaac
    12901 gcccagaagc gtggaaccat cctggacaaa ggaatcatta acaacaaagt ctcggagcga
    12961 ttgttccgcc tcctcgaaga aagcggcgtg ccgacacatt tcgtggagcg aaagagcgat
    13021 cgcgaaatgc tcacgaagaa ggtgacgatc gtgccggtcg aagtggtggt gcgaaacgtg
    13081 atcgcaggca gtctggccaa gcggttggga ctcaaggaag gcgaagcgat tcagccgccc
    13141 atcgtcgagt ggtactacaa aaacgacggc ttgggagatc cgttgatcgc cgatgaacac
    13201 gtccgcctgc ttaagctcgc gacgccggaa cagatggcgg aaattaaagg actggcatta
    13261 aaagtgaata gcgtgctgca gccgttcttt gcagaacgcc ggatggtcct tgtcgatttc
    13321 aagcttgagt ttggcgttca taacggccgg ctcattctgg ccgatgagat ttcccctgac
    13381 acgtgccggt tttgggacca ggcgaccaga gagtcgatgg acaaagaccg gtttcggaag
    13441 gacttgggga agattgagga ggcgtatcag gaagtgttga agagagtatg cgggtaacag
    13501 gggtgcggag gcatgttgcc cttcgcttca cagcctcacg gagcgaccct ttcggactcg
    13561 gcctggcgga ctccttcgga aattccc
//
LOCUS       DASSCQ010000098        19900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_350458, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000098 DASSCQ010000000
VERSION     DASSCQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 19900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19900
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350458"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1052)
                     /locus_tag="VFH05_06865"
     CDS             complement(27..1052)
                     /locus_tag="VFH05_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6677969.1"
                     /translation="MTAAKLRVLGAVLGVAGIWGLLAGGCASEQERRGKELYAHYCMH
                     CHGENGRQNEGFNWSSMPDPKPKDLSNKSEMSTFKDEDVFNTISRDMKDTSPGGDKIG
                     DDDFAVPTMPTFKYTLSEDEIWAIVGYVRTLHGMKLEFNVEERKKQLADAVKTAQDKF
                     EQAKQAYEAAEKRASEEAEKKSAALKKDVDVDESAYAKEQAAMAQAKKELDAAQATLA
                     YFSTRPGKGLNVARPDLTLKPEQSAPLAEVGKRLYANKYGCNGCHSLAGEGGKVGPAL
                     DRAGFRLNGTWIYRWVKNPQSMKPETRMPALGLSDADAKAVTMYLATLRAPKAEEAAP
                     PTAKPAS"
     gene            complement(1049..1876)
                     /locus_tag="VFH05_06870"
     CDS             complement(1049..1876)
                     /locus_tag="VFH05_06870"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HET6677970.1"
                     /translation="MKAGRSNIISSVVACVILVAGCFIALSVSAQESVAVRAVMTKEP
                     MPLDDPNAAVWSSAPQAQFPMSPQVHWQNRIQEVTVKDLKVRALNDGKQVAVLLEYAD
                     PTEDPADAAALEFMVGDTKAHFAHGQPMAQVEGGPVNIWFWRNKDGKGVDMSAKGFGT
                     LKPQTHQDVTAKGVYANGTWKVVFSRPLTTEHPNEDMQLKPGEFINIAFAVWDGRKDG
                     AGDLVEKGSQKAVSSWWYFRAEAPPDYSGYIYAALAMGLALAFQFVLIRKLKKGQST"
     gene            complement(2028..2744)
                     /locus_tag="VFH05_06875"
     CDS             complement(2028..2744)
                     /locus_tag="VFH05_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6677971.1"
                     /translation="MGSLTKPLILVVVIYLLLKFVVPNIPGSAPLPSSLIFLYLMLTV
                     AGIVIYYTMSGESKDAFWGPIQRFLTGENIGALQTARYAVLILFPLLVGWQTYGSTAV
                     SDAPPPENRTIHPAPPGEYTGLSNPVAKTPENIMQGKGFYAAFCSPCHGGNFDGKGPA
                     ARGFNPPPANFSDPTTIAMLQESYLFWRIKKGGVGLPIEGMPWKSAMPRWEVELPDEW
                     IWKIILGEYDGAHQSPRTWE"
     gene            complement(2755..3378)
                     /locus_tag="VFH05_06880"
     CDS             complement(2755..3378)
                     /locus_tag="VFH05_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6677972.1"
                     /translation="MSEVVKLQLIGLTVVGMGIVILLFVRAQFARVIGFVAIVLGLFS
                     LVALAVPQMASLPPAEEKFDIATVKTPTDMAAIGQKIFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSREFIFESLTQPQAYIYLDYRHEGPPKEYPARMPYINKNPIGLTKNEI
                     LSVIAFLQQMSGEPISVNPSELEVPGQAPKAPVKAEDAAPVAVAHAR"
     gene            complement(3415..5268)
                     /locus_tag="VFH05_06885"
     CDS             complement(3415..5268)
                     /locus_tag="VFH05_06885"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6677973.1"
                     /translation="MRHQGGHLQRLLRALRRQGGWLPAWVFAAGLLAVPALGLAAEGT
                     SAGPVEYRDIPGIGSRNIVWVIAQLHLLLAGFVLGVPIFAWLCEIVGWKSGEKRYDKL
                     AKEFTKLLTSSYATTALFGGILLFLLIGFYPKLMNYLTDIFFPSFVFYCILFLLETAT
                     LYLYWYGWDAMQDGGKKTFHIFLGFLLNLFAFFIMIVPNSWATFQASPVVIAEGTDIQ
                     RAWAATWNPTWWPVNIHRLIANVVLGGYICGAYAGIRYLAARNAEERDHYDWMGYVGN
                     FIGVFGLLPLPFAGYWLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYY
                     FWLGITHRIPGSEGQYKKPIMTMLVILLLCLGVWMTPHSLVASLEEARKMGGTHHPLL
                     GDFGVMSAKMTVANLMILVTFMSFIMYWRAGKQETATWAKVARSVMGAVLVLAGIAVI
                     VLGVWGYFVPAIIRINYFSTSQVLIVLFVMLTITPLTALLLKSARTTTEMVWGRMPPR
                     AGYALVLNAVMVILLMTLMGYARSSSRVHWHVYGVMRDSSQYAFSPALGYAAALMALN
                     TFLFCMLVAFIFWVATMGDKGKAAHGASKGELPHGMPAMAGGAPHHMDQQS"
     gene            complement(5297..6271)
                     /locus_tag="VFH05_06890"
     CDS             complement(5297..6271)
                     /locus_tag="VFH05_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677974.1"
                     /translation="MSDDSLSPTKIGLGILWSAFWTALPIKLAFAIVFMAMDLMHLEN
                     RLGLAFLMLLASPVTVFALPIISMGLESHIGEGAGIAILFLLAIPVDIWALGLVGRTY
                     FLEKFRKEPPDGLGLTLWWKSAVVGAIVLPIIWFLVSTVTSTAISMAHSLLEMEIFKG
                     IPVAERISVELTLWGSVSAIVLLVLLLIGVSLVGRIIRGTAEAARPVAENYQSIVTRW
                     DLMRVPADQGLTLTALTLGGVVLSVLFWASLPVTTPHPHECCKKPDVKAEPPFKPVET
                     LNKDEKMMAQLELQVSALEKQMAEAEKDKGKGKPKAAKEAAPPAPKKP"
     gene            complement(6301..7413)
                     /locus_tag="VFH05_06895"
     CDS             complement(6301..7413)
                     /locus_tag="VFH05_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HET6677975.1"
                     /translation="MAQSSLPSTEPTVIEKVVAFVDERIGLKQMQAKMLNEPVPGGSR
                     WAYVFGSVLLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHDVDYGWFLLSYHFWG
                     STAMVVCVFAHMSQVFLWGAYKPPREMIWLVGLALFGIVMGFGFTGYLLPWDQRAYWA
                     TTVGVEIMDKTPLVGDFMARFLKGGPTPGQMTLSRFFVIHVMILPAALMGLAGLHLFL
                     FRAAGPAGPFRGTPEEIRAKTDYFFPRQVWKDIVGMAVVFASICALAFWEPVVLLEEA
                     TPDPGEYHPEPEWYFLFLFQLLRLKVFAGEFGQFLGAIALPGAFMALLAALPFIDRDP
                     ERNIFKRPIALISWIAVMTAILLFTVSAIINREFLD"
     gene            complement(7414..8346)
                     /locus_tag="VFH05_06900"
     CDS             complement(7414..8346)
                     /locus_tag="VFH05_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET6677976.1"
                     /translation="MQPQLKSKVRCTDREIGEVTKVIVDPLSHQISHIVVSMNGSGER
                     QVAMALVQTVTDSTVQLRNTSSEIVALPPFNRAEYVSTHEVEIAHLEDKLHVTPGEVL
                     VPFPELEKNVKRRTFFMNFTHAIGFLIGLPIAFPILRYLMKPMYAPFDNAWLKIGNVG
                     KIKSDDIGVQFKYKKKVKEAYLPEAEVEKNIWILKATPAVLEKVYQGKDMEFRDATGK
                     PVWTNKKDIPYIAFSGKCPHLGCGYKWRKHKVLGQVFLCPCHLSIYDASGKVLDGPAP
                     RPLDALPIKVSASGEVQIIDMEFKAGTKSQIRIV"
     gene            complement(8357..9163)
                     /locus_tag="VFH05_06905"
     CDS             complement(8357..9163)
                     /locus_tag="VFH05_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6677977.1"
                     /translation="MGNVVKKVVIGLIVGGALFGITRALEFPTVFQWMFFAYALLGTA
                     VFILLDAPPVKPVGGVKALGALVVFYIVLCVVYIAGASLWPQYDPEDEKGKIEKILKP
                     KRAATEQGKAEELLARAKALDEQAKALAARIKALGGDQAPGAQAAGGAGTPPSTTGAA
                     GGDLLKIGEDQWQLQECYNCHKLRGEGGKKRGPELDNIGNLLTVDDIKQKILDPKSWM
                     AEGFEKEYEKGKMPDKYKDLMEEQDVVALASWLVTFKNTSVNTPKPIKKK"
     gene            complement(9200..10903)
                     /locus_tag="VFH05_06910"
     CDS             complement(9200..10903)
                     /locus_tag="VFH05_06910"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6677978.1"
                     /translation="MKTWQRGLLACCALLALLGGAAFAQTPGAPPVEFPYTGNRTAVW
                     IVAQLHILFAAFILGAPIFVVISEWLGYRKHDPRYDRLAKEVMKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLVFAVIYPLLFIGETILLYMYFYSWDAWKGEKKARH
                     IALGILLNLVGTITLFVIDGPTSFMNTPVKAEGISPMDFLATASLWDKIYNYSWMPMN
                     LHRLVGNVTFGGFITGLVAAYMYMAAKKEDERAYYDWMGFVGNLIGVGALLFLPFMGY
                     LLAYELCDYDASICPYMMADQLSMFFEMQGAMVGLIFLASNYYIWLSMKRIEGVERVR
                     MTIFAPMVMVALPFVMTWAWTMFPVPDPLSLAILVPLALLPVVLGRFIPWTVSARTVI
                     KVGFLMVVVGDAIWLTPHGFVPTGAKLVADLELPSDWNFLALMPAKNSAAFTLVFVTV
                     VNYIIYNRAISQGTIVWGKIDFVSQFVLVFLAFSAIWTMGLMGAVRSLLRKYFHTYNL
                     LPDFTPESFTPTLSYSAWWITAITVAFYVVVSFAIFVTLRPAEAKGLAPEGTPAPAGA
                     K"
     gene            complement(10957..11463)
                     /locus_tag="VFH05_06915"
     CDS             complement(10957..11463)
                     /locus_tag="VFH05_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6677979.1"
                     /translation="MTQSRTLQALLMGVGLIMSGCGESGEGPVVPPPPAPAEYADKHM
                     PADWWGDAAKAEEGRKLFIGETNPDVNCASCHGKDGKPVKAGARDFRNGERMKLYSDS
                     VWFWRISEGVPNTKMKAWKSKLSDEDRWKLVLFERNFGLSGKKWDDGKKEWADAGNMA
                     VAQAGAAK"
     gene            complement(11637..12122)
                     /locus_tag="VFH05_06920"
     CDS             complement(11637..12122)
                     /locus_tag="VFH05_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677980.1"
                     /translation="MCLVTAFPSVGSATHEADHRFTVEGFVCGSDGKGMADTDVLVKD
                     TKISYGQVVKTDGDGHYKATFHLHNENLGDALLVEAKGEQQNHTIQFDPKDLESERKV
                     HIHFGTGCVRDVNSPPTWVWWGLGGTAAIAIGAVGFSKIARARRKQGQKRGKSQSKRK
                     H"
     gene            complement(12370..12762)
                     /locus_tag="VFH05_06925"
     CDS             complement(12370..12762)
                     /locus_tag="VFH05_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677981.1"
                     /translation="MTDTKDHTPADAGSPEGRVFRPSSSAETAEAVELAFDYRGDVTL
                     ELKSGETLTGYVFNRDHAGADPWIEVLSAADSDSRRIAYRDILSIGFTGVDTANGKSW
                     ETWVSKKQSDRLAEAARVEADARTRGHL"
     gene            complement(12817..13833)
                     /gene="hpnH"
                     /locus_tag="VFH05_06930"
     CDS             complement(12817..13833)
                     /gene="hpnH"
                     /locus_tag="VFH05_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HET6677982.1"
                     /translation="MAVPLSQMYTVASYVLTQKFKGVKRYPLVLMLEPLFRCNLACAG
                     CGKIQYPDHVLDKRLTPEQCWAAARECGAPIVSIPGGEPLIHPEMATIVKGLVAQKRY
                     VYLCTNAILLERKLDEYQPSKYLTFSIHMDGLKDEHDLAVCRDGVYDVAVRAIRAALK
                     RGHRVTTNTTLFDDANPERVRKFFDEMMALGVEGMMISPGYSYQKAPDQQHFLKRART
                     KELFSKILSQPRRGWQFNQSPLFLDFLMGRRDYQCTPWGNPTYNVFGWQKPCYLLQEG
                     YAKTFRELIETTQWQDYGTTKNEKCADCMVHCGYEASAVEDTFGSLSGFARTAKLTLL
                     PTSR"
     gene            complement(14063..14138)
                     /locus_tag="VFH05_06935"
     tRNA            complement(14063..14138)
                     /locus_tag="VFH05_06935"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14103..14105),aa:Cys,seq:gca)
     gene            complement(14268..15446)
                     /gene="ispG"
                     /locus_tag="VFH05_06940"
                     /gene_synonym="gcpE"
     CDS             complement(14268..15446)
                     /gene="ispG"
                     /locus_tag="VFH05_06940"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET6677983.1"
                     /translation="MHISRRQTRQIQVGTVKIGGDAAISVQSMCSTDTRDVPATVEQI
                     RQLESAGCELIRVAVPDEEAAAALPKIKAAMTVPLIADIHFDHRLALKAAEIVDCVRI
                     NPGNIGAWWKVEEVIKAVNARGIPLRVGVNGGSLERPLLDKYGWPSPEALAESALNAV
                     HALEDVGFTNLKVSLKASDVHHAIDAYWLFAHQSNYPLHIGITEAGTAMTGAVKSSIG
                     LGYLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLSHIKTSLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLVRKV
                     PMEELMDTLIHEVELLAKEKDAESSGEPVSPSSEGWEALPGQSDRTAGKEIPVLSNKR
                     "
     gene            complement(15465..17414)
                     /gene="dxs"
                     /locus_tag="VFH05_06945"
     CDS             complement(15465..17414)
                     /gene="dxs"
                     /locus_tag="VFH05_06945"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET6677984.1"
                     /translation="MSILKTVHSPADLKRLKPAQFPKLCQELREQIIGAVSNVGGHLA
                     SNLGVVELTVALHYLLDTPQDRIVWDTSNQAYAHKLLTGRREQFHTLRQYGGLSGFCK
                     REESVYDTFNAGHAGTGVSAAYGMVEAREQQGQKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGLGKDFLVILNDNQMSISKNVGAISSYLNRTFTGEFYTKMREETGQLLRKIPHIGYD
                     VEKFARRAEELAKGAILPGLLFEELGFRYAGPIDGHNFEHLLPTLENVLKMRGPVLLH
                     VITKKGLGYAPAEKNPVWFHACPPFVRETGAPAKKAVRPSYTQIAIDSLIRLAREDRR
                     VVAITAAMCEGTGLNAFEKEFPDRLYDVGIAEQHAVTFAAGLAAQGMRPVVAMYATFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDYAYLRHMPNMVIMAPKDE
                     NELQHMLKTALQYDGPASVRYPRGVSLGVTMDRELSALPIGKAELLRDGTDVAIIAIG
                     VSVWPAVQAAEQLSQEGLSTAVVNARFAKPLDQELIVEVAKRVRYVVTVEEGCKMGGF
                     GSAVLEALSDAGVTGVRTKILGLPDWYIEQGPQDLLRERYGLTTDGITRSVKELVSKS
                     PAESREAEYFSAAIDHLPHGDEQGS"
     gene            complement(17599..18627)
                     /locus_tag="VFH05_06950"
     CDS             complement(17599..18627)
                     /locus_tag="VFH05_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HET6677985.1"
                     /translation="MLYSDDRRGSSTRRALVAGLIGMACLHPSSASAEGFGPFPVRNF
                     NPLQQLVLNMPGDRAAVLEPGAWDVRLELAETAAIYSQAPPGATASVKFETLRNGLFL
                     RYGATRKLEIALEVPVLYRYTGFMSGAIEAVERATTGISPARQALSHVSYAYSISRNG
                     QQIVSGTKGAVGLGDSTLQAKYQLLNETSSMPALSLRTAVKLPTGNEREFFGSGSPDF
                     GIGIAAEKRLGGRWILYGNLSTVLPTGRIAGLPLYPTISGMAATEYLWSDNLSLTLQF
                     DYYTTPFRHVGLEVFDKGATEVVAGFNYRIAEQWLWQMYAVENVDFITGGAADFTVAV
                     LFTYRYRT"
     gene            complement(18629..19831)
                     /locus_tag="VFH05_06955"
     CDS             complement(18629..19831)
                     /locus_tag="VFH05_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET6677986.1"
                     /translation="MTTLRGIAIFFVCLVVGMTAVWVRPATADTQIFPIPSASTSKND
                     GSDAGLIVPILITDPDGELKYLMAPMVIKNSIVGTRGTFNLFRYDTGGRQMQFIASLT
                     EKIERKLVFNYIDPAFSDGRYFLNFGGTFFKNATSRFFGLGQTTVEADETNYTAREAR
                     ANWRFGVYANEVTQIAVGQRVRQVSLQRGATDLPFTGDLYPTVEGVQGETIIIGHRAT
                     FYYDTRNNLISPTDGMAVTAYAEVNQNLSNSDSPVFSRYELEVKKLFPSESKRAILII
                     RADLQATLGSQVPFFEQSSLGGQNNLRGYGVDRFIDKHLIAASVEERIHLVRAKIAGV
                     AADFEMATFLDTGQVFNDFKDVSFKDYRMTPGIGFRGIVRPNVVGRIDYGYSKEGGAI
                     FAGLDFPY"
BASE COUNT         4205 a         5994 c         5579 g         4122 t
ORIGIN      
        1 aatacgccac gctcgagggt tacggctcag ctcgcgggct tcgcggtcgg cggcgccgcc
       61 tcctcggcct tcggcgccct gagcgtcgcc aagtacatcg tcacggcctt cgcgtccgca
      121 tcgctcaagc ccaatgccgg cattctggtt tccggcttca tgctctgggg gttcttcacc
      181 caccgataaa tccatgtgcc gttgagtcga aagcccgctc gatccagcgc cggtccgacc
      241 tttccacctt ctcccgccag gctgtgacac ccgttgcagc cgtacttgtt ggcatacagc
      301 cgtttgccga cttccgccaa cggggccgac tgctccggct tcaaggtcag atccggcctt
      361 gccacgttga gacccttgcc cggcctcgtc gaaaaatacg cgagcgtcgc ttgagcggca
      421 tcgagttctt tcttcgcctg ggccatggcc gcctgttcct tcgcataggc ggactcgtcc
      481 acatccacat cttttttcag cgcggcactc ttcttttccg cctcctcgct ggctcgcttc
      541 tcggctgcct cataggcttg cttggcttgc tcaaacttgt cctgggccgt tttgacggca
      601 tcggccaatt gcttcttgcg ttcctcaacg ttgaattcca gcttcatgcc gtggagtgtg
      661 cgcacgtagc caacgatcgc ccagatttca tcctccgaga gggtgtactt gaacgtcggc
      721 atcgtgggca cggcaaaatc atcgtcaccg atcttgtccc cgcctggact cgtgtccttc
      781 atgtcccgtg agatcgtgtt gaacacgtct tcatctttga aggtgctcat ctcggatttg
      841 ttggacaagt ccttgggctt gggatcgggc atggaggacc agttgaatcc ttcgttctgg
      901 cggccgttct cgccgtgaca gtgcatgcaa taatgggcgt acagttcctt gccccgcctt
      961 tcctgctcgc tcgcgcaccc gccggccaag agaccccaga tcccggcaac gcccaacacc
     1021 gctcccagta cccgtagctt tgcggccgtc atgttgactg ccctttcttc agtttgcgaa
     1081 tcaacacgaa ctgaaaggcc agcgcgagcc ccatggccag cgcggcatag atgtatccgg
     1141 aatagtccgg cggcgcttca gcgcggaaat accaccagga ggacaccgct ttctgcgacc
     1201 ctttttcgac caaatcgccc gccccatctt tgcggccgtc ccacacggca aaggcgatgt
     1261 tgatgaattc gcccggcttc aattgcatgt cttcgttggg atgttcggtg gtcagcggac
     1321 gggaaaatac gaccttccag gtcccgttcg catacacgcc cttggccgtt acgtcttgat
     1381 gggtttgcgg cttgagcgtg ccgaagccct tggcactcat gtcgacgcct ttgccatcct
     1441 tgtttctcca aaaccagatg ttcaccggcc cgccttcgac ctgggccatc ggctgcccat
     1501 gggcaaaatg cgccttcgtg tcgccgacca tgaactcgag ggcggcggcg tcggccggat
     1561 cttcggtcgg gtccgcatat tctaagagca cggccacctg cttgccgtca ttcaaggcgc
     1621 gcactttcaa atccttgacg gtgacttcct gaatgcgatt ctgccaatgt acttgcggcg
     1681 acatcgggaa ctgggcctga ggggcgctgc tccacacggc ggcgttcgga tcgtccagcg
     1741 gcatcggttc cttggtcatg accgcgcgaa cagccacgct ttcctgggct gaaacagaca
     1801 gcgcgatgaa acatcccgcg acaagaatga cacaggcaac aacagatgaa atgatattgc
     1861 ttcgtccagc tttcatgaca tccgtcctct ctcttgctct ctaccagagg gtggggctgt
     1921 gtgccttcta ctgcgcgcgt tcaccgagca cctccaactg tttaaacatt cgatcaatct
     1981 ggtgtgccgc ggtgcgcgag cacagaaggc atacagccct accctcatca ctcccaggtg
     2041 cgcggggatt gatgggcccc gtcgtactcg cccaaaatga ttttccagat ccattcatcc
     2101 ggcaactcca cttcccatcg tggcatcgcg gatttccagg gcatgccttc gatcgggagc
     2161 ccgactccac cttttttgat gcgccagaag agatagctct cctgcagcat cgcaatggtc
     2221 gtcggatccg aaaagtttgc aggcggcggg ttgaagccgc gcgccgcggg ccccttgcca
     2281 tcgaaattgc cgccgtgaca gggcgagcag aacgctgcgt aaaacccttt gccctgcatg
     2341 atattttccg gagtcttggc gaccggattc gagagacctg tgtattcgcc cgggggcgcc
     2401 ggatggatgg tgcgattctc tgggggtgga gcatcactca cggcggtgct gccgtaggtc
     2461 tgccagccga ccaggagagg aaacagaatg agtacggcgt agcgtgcggt ctgcagcgcg
     2521 ccgatgttct cgccggtgag gaatcgttgg atcggtcccc agaatgcgtc cttcgattcg
     2581 ccgctcatcg tgtagtagat gacgatgccc gccaccgtca gcatcaggta cagaaaaatc
     2641 aggctcgagg gcaacggtgc cgatccgggg atgttcggca cgacgaactt caacaacaga
     2701 taaatgacaa cgacaagaat gagtggtttg gtcaacgagc ccatgggcgc ctcctcaacg
     2761 tgcgtgtgcg accgcgaccg gcgcagcgtc ctcggccttg accggcgcct tcggcgcctg
     2821 tcccggcact tcaagctcag aaggattcac cgagatcggc tcaccgctca tctgttggag
     2881 aaaggcgatg accgacagga tttcgttctt cgtgagtccg atcgggttct tattgatgta
     2941 gggcatccga gcaggatatt ccttgggcgg cccttcatgg cgataatcca gatagatgta
     3001 ggcctgcggt tgcgtcagac tttcgaaaat gaattcgcgg ctgagcttgg cgccgatccc
     3061 cttcaaatcc gggcacctgg ccgactcgct cgggcctatg gaatggcaga gcgcacattg
     3121 tcccttgctg aagaagattt tctgaccaat cgcggccatg tccgtcggcg tcttgaccgt
     3181 cgcgatatcg aatttttctt ccgcgggcgg cagcgatgcc atctggggaa cggccagggc
     3241 caccagcgag aagagcccca gcacaatggc gacgaatccg atgacccgcg caaactgagc
     3301 gcggacaaac aataggatga cgatgcccat gccgacgact gtcagaccga tcaactgcag
     3361 cttgacgact tcactcatag agaccccgag ttagtggtgt atgacccctc accctcacga
     3421 ctgctggtcc atgtgatgcg gagccccgcc ggccatggcc ggcatgccgt gtggcaattc
     3481 ccctttggac gcaccgtgcg ccgctttacc cttgtctccc atggtcgcga cccagaagat
     3541 gaaagccacc agcatgcaaa acaggaacgt attgagcgcc atgagcgccg ccgcatagcc
     3601 gagcgccggc gaaaaggcat attgcgagga gtcacgcatg actccgtaca catgccagtg
     3661 gacgcgcgac gatgaacggg catatcccat cagagtcatc agcaagatca ccatgaccgc
     3721 attgagtacg agcgcatacc cggcacgcgg aggcattcgg ccccacacca tttcggtagt
     3781 cgttctcgcg ctcttgagta acagggccgt gagcggcgtg atcgtcaaca tcacaaacaa
     3841 cacgatcaac acttgggaag tcgagaagta attgatacgg atgatggcgg gcacgaagta
     3901 gccccacacg ccgaggacga tcaccgcgat gcccgccagg acgaggaccg ctcccatgac
     3961 cgaccgcgca accttggccc aggtcgccgt ctcctgcttg ccggcccgcc aatacatgat
     4021 gaagctcatg aacgtcacga ggatcatcag gttggccacg gtcatcttgg cggacatgac
     4081 gccgaaatcc ccgagcagcg gatggtgcgt gccgcccatc ttgcgggctt cctctaagct
     4141 cgccaccagc gagtgaggcg tcatccagac acccaggcac aacaataaaa tcaccaacat
     4201 cgtcatgatg ggttttttgt actgtccttc ggaacccggg atccggtggg taatgccgag
     4261 ccagaaataa tagttcgagc cgaggaatag caccccgatc agcatcgctt gcagaatgaa
     4321 cagccaggac agaaatccac ccatgagcgt gatgcccatc tgctggttgt actggtagat
     4381 ctcccgcatc agccaatatc cggcaaaggg aagcggcagg agaccaaaca cgccgatgaa
     4441 gtttccgaca tagcccatcc agtcgtagtg atcccgttcc tcggcgtttc gggcggccag
     4501 ataccggatg ccggcatagg cgccgcagat gtagccgccg aggacgacgt tggcaatcag
     4561 gcgatggatg ttcaccggcc accaggtcgg attccaggtc gccgcccaag ctcgctggat
     4621 gtccgttcct tccgcaatga cgacaggact ggcttggaat gtcgcccagg agttcggcac
     4681 gatcatgatg aagaaggcga agagattcag caggaacccg agaaaaatgt gaaaggtctt
     4741 tttccctcca tcctgcatgg cgtcccagcc ataccaataa agatagagcg tggccgtctc
     4801 gagcaggaag aggatgcagt aaaagacgaa ggaggggaaa aagatatcgg tcagataatt
     4861 catgagtttg ggatagaacc cgatcaacag gaacaacagg attccgccga agagtgcggt
     4921 cgtcgcgtag gaggaggtca gcagtttcgt aaactctttg gccagcttgt cgtaccgctt
     4981 ctcgccgctt ttccaaccga cgatttcgca gagccacgcg aagataggca cgccgaggac
     5041 gaaacccgcc aacagcaaat ggagctgggc gatgacccac acgatgttcc ggctaccgat
     5101 gcccgggatg tctcgatact ccaccgggcc ggccgacgtc ccctccgccg cgagcccgag
     5161 tgcgggcacg gccagcaggc ccgctgcaaa cacccacgct ggaagccatc ccccctgacg
     5221 gcgaagagcc cggagcaatc gctgtagatg tccgccctgg tgtcgcatgg tactcacctc
     5281 atcctgtgaa gcacgattac ggtttcttgg gcgcaggagg agcggcctct ttcgcggctt
     5341 tcggctttcc cttgcctttg tccttctcgg cttcggccat ctgcttctcc aacgcgctga
     5401 cctgaagttc cagctgtgcc atcatctttt catctttgtt gagggtctct accggcttga
     5461 acggcggctc ggccttgacg tccggtttct tgcagcactc gtgcggatgc ggagtggtca
     5521 ccggaaggga cgcccaaaac aggaccgaga gcaccacgcc gccgagcgtc agcgccgtca
     5581 gcgtcaggcc ttgatctgcc ggtacacgca tcaaatccca gcgagtgacg atgctctgat
     5641 agttttcggc caccgggcgg gcggcttctg cggtgccgcg aatgatcctg ccgacgagcg
     5701 acacgccgat gagcagcagc acgagcagga cgatggccga cacgctcccc cagagggtca
     5761 gctcgacgct aatccgctcg gccaccggaa tgcccttgaa gatctccatc tccaaaagcg
     5821 aatgggccat cgaaatcgcc gtggaggtca ccgtactgac gagaaaccag atgatgggca
     5881 agacaatcgc gccgacaaca gcagacttcc accacagcgt cagtccgagc ccatccggcg
     5941 gctccttgcg gaatttctcg aggaaatagg tgcgacccac gagtccaaga gcccagatat
     6001 cgacgggaat cgccagcaga aaaaggatgg cgatgcccgc gccttcgccg atgtgcgact
     6061 ccaaacccat ggagatgatc ggcagcgcga agacggtcac gggactcgcc aacagcatca
     6121 agaaggccag ccccagcctg ttttccaaat gcattaaatc catagccatg aacacgatgg
     6181 caaaggccag cttgatcggc agagccgtcc aaaacgcgga ccagagaata cccagaccga
     6241 ttttcgtggg agagagagag tcgtcgctca tatgattgat aaggccggct cgcgccgcac
     6301 ttaatccaga aattcgcggt taatgatcgc ggacaccgta aacagaagaa tcgccgtcat
     6361 caccgcaatc cagctaatga gggcaatcgg acgcttgaag atgttccgtt cgggatcgcg
     6421 atcgataaac ggcaacgcgg ccagcagggc cataaatgcg ccgggcaacg cgatggcgcc
     6481 aaggaactgg ccgaactcac cggcgaatac tttcaagcgc aacaactgga acaagaacaa
     6541 gaagtaccat tcgggttccg gatggtattc gccagggtcg ggtgtcgctt cttccagcag
     6601 cacgaccggt tcccaaaagg ccaaggcaca gatgctggcg aagaccacgg ccatcccgac
     6661 gatgtctttc cagacctgcc gtgggaaaaa gtaatcggtc ttcgcgcgga tttcttccgg
     6721 tgtcccgcga aagggaccgg ccgggccggc cgctcggaac aggaacagat gaaggcccgc
     6781 aagccccatg agggcagccg gcaagatcat gacgtggatg acaaagaacc ggctgagggt
     6841 catttggccc ggcgtcggac cgcccttgag gaatcgggcc atgaaatccc cgaccagcgg
     6901 ggtcttgtcc atgatctcca cgccgacggt tgtggcccag taggcgcgct gatcccacgg
     6961 aagcaggtag ccggtgaacc cgaaccccat gacgatgccg aacagcgcca ggccgaccag
     7021 ccagatcatt tcgcgaggag gtttgtaggc gccccagaga aagacctgcg acatgtgggc
     7081 aaagacacag acgaccatgg cggtggaacc ccagaagtgg taactcagga ggaaccagcc
     7141 gtaatccacg tcatgaatga tgtactgcgt gctcgcatac gcgtgatcgg cggtcggcac
     7201 gtaatagaac atcaagagaa tgcccgtgac cgcctgcatg atgaaaatga acaacagcac
     7261 ggagccaaac acgtaggccc aacgggagcc gcctgggacc ggctcgttga gcatcttcgc
     7321 ctgcatctgc ttcaagccga tgcgctcgtc gacgaaggcc acgactttct cgatcacggt
     7381 gggttcggta ctagggagcg acgactgtgc catttaaaca atccgtattt gggacttcgt
     7441 gccggctttg aattccatgt cgatgatttg gacttcaccg ctcgcggaca ctttgatcgg
     7501 cagcgcgtca agcggtcgag gcgccgggcc atcgagcacc ttgccggacg catcgtaaat
     7561 gctcaagtgg caggggcaga gaaagacctg gccgagcact ttatgcttcc gccacttgta
     7621 cccgcagccc agatgcgggc acttgcccga aaaggcgata taggggatgt ctttcttatt
     7681 cgtccagacc ggtttccccg tcgcgtcacg gaattccata tccttgccct ggtagacctt
     7741 ctcgagcacc gcgggggtgg ctttcaaaat ccagatgttc ttttcgacct cggcttcggg
     7801 cagatacgct tctttaacct tcttcttgta tttgaactgg acgccgatgt catcggactt
     7861 gatcttcccg acgttcccga tcttcagcca cgcgttgtca aacggggcat acatgggctt
     7921 catgagatac cggaggatcg gaaatgcgat cggcagccca atcaagaatc cgatggcatg
     7981 ggtaaaattc atgaagaacg tacggcgctt gacgttcttt tccaattcag ggaagggcac
     8041 caggacttcg cccggggtca catgcagctt gtcttccaaa tgggcgattt cgacttcgtg
     8101 cgtactgaca tattcggccc ggttgaacgg cgggagcgcc acgatttccg aggaggtgtt
     8161 gcgtagctgc accgtactgt ccgtcacggt ctgtacgaga gccatcgcga cctgccgctc
     8221 gccactcccg ttcatggaga cgacaatgtg gctgatctga tgcgagagcg gatcgacgat
     8281 gaccttcgtc acctctccga tttcgcggtc tgtgcagcgg accttcgatt tcagttgcgg
     8341 ttgcatatgc ctctgcttac ttcttcttga ttggcttcgg cgtgttgacc gaggtgttct
     8401 tgaaagtgac cagccacgag gcgagcgcaa ccacgtcctg ctcttccatg agatccttgt
     8461 acttgtcggg catcttgccc ttttcgtact ctttctcgaa gccttccgcc atccagctct
     8521 tgggatcgag gatcttctgc ttgatgtcgt cgacggtcaa aagattaccg atattgtcga
     8581 gctccggacc gcgttttttc ccaccttcgc cgcgaagctt gtggcagttg tagcactcct
     8641 ggagctgcca ttggtcttcc ccaatcttaa ggaggtcccc gccggcggcg ccggtcgtgg
     8701 atggcggggt accggcaccg cccgcggcct gcgctccagg tgcctgatca ccgcccaacg
     8761 ccttgatcct cgctgccagg gcttttgcct gctcatcgag cgccttcgcc cgggcgagca
     8821 attcttccgc ctttccctgt tccgtcgccg cgcgtttggg cttcagaatt ttttcaattt
     8881 ttcctttttc gtcttccgga tcgtactgcg gccagagcga ggcgccggca atgtacacga
     8941 cgcacagcac tatgtagaac accacgagcg cgccgagggc tttgactccg ccgaccggtt
     9001 tgacaggcgg cgcatcgagg aggatgaaca cggcagtccc gaggagggca taggcgaaga
     9061 acatccactg aaacaccgtg gggaattcca gcgccctcgt gatgccgaag agcgcgccgc
     9121 cgacgattaa cccgatgacc accttcttga ccacgttccc catagagatc ctcttccctg
     9181 catgaaggtt cgacccgact tatttggcgc cagcaggagc cggagtccct tccggtgcaa
     9241 gacccttcgc ctctgccggc ctcagagtga caaaaatggc gaagctgacc accacgtaaa
     9301 aggccacggt aatcgcggtg atccaccagg ctgaatagga caaggtcggg gtgaaggact
     9361 cgggggtaaa gtccggaagc agattgtaag tatgaaaata ttttcgtaac agtgaccgga
     9421 ccgcccccat caagcccatc gtccaaatgg cactgaacgc gagaaacacg agcacgaact
     9481 gcgatacaaa atcaattttc ccccacacga ttgtgccctg gctgatcgcc ctgttgtaaa
     9541 tgatgtagtt cacgactgtg acgaacacta acgtgaaggc cgcggaattc ttcgccggca
     9601 tgagggcgag gaagttccaa tccgaaggca gctccaaatc cgccaccagt ttggccccgg
     9661 tgggtacgaa gccatggggc gtcaaccaga tggcatcccc gacaacgacc atcaagaacc
     9721 ccaccttgat gacggtccga gcggagactg tccacgggat gaagcggccg aggacgactg
     9781 gcagcagggc cagtgggacc agaatggcga gtgagagggg atccggcacg gggaacatcg
     9841 tccatgccca ggtcatgaca aagggcaggg ccaccatgac catcggcgca aagattgtca
     9901 tcctgacccg ctccactccc tcaatgcgct tcatgctcag ccagatgtaa taattactgg
     9961 caagaaagat gaggcccacc atggctccct gcatctcgaa gaacatcgac aactggtccg
    10021 ccatcatata cggacagatc gacgcatcgt agtcgcagag ctcataggcg agcagatacc
    10081 ccatgaacgg cagaaacaac agcgcaccga ccccaatcaa atttcccaca aaccccatcc
    10141 agtcgtaata tgcccgctca tcttccttct ttgccgccat gtacatatag gcagccacaa
    10201 gaccggtgat gaacccgccg aacgtgacgt tgcccaccag gcggtgcaaa ttcatcggca
    10261 tccagctgta gttatagatc ttgtcccaga ggctcgcggt ggcgagaaag tccatgggcg
    10321 agatgccttc cgccttgacc ggagtattca taaacgacgt ggggccgtcg atcacgaaca
    10381 gggttatcgt gccgactaaa ttcagaagaa tacccagggc gatatgcctg gccttcttct
    10441 ctcctttcca ggcatcccac gaatagaaat acatgtagag aaggatggtc tccccgatga
    10501 agagaagcgg ataaataacc gcgaagacca gaaagaagtg attgatgagc caggtggtga
    10561 actgcgggta ggtcgccagc aagacaaaga tgaataaacc gccggtcagg gcggtcatgc
    10621 tgtagagaat gaccgtgacc ttcatgacct ccttggccag acgatcgtag cgcggatcat
    10681 gcttccgata tccgagccat tcggagatga cgacgaagat cggcgcaccg aggatgaatg
    10741 cggcaaacaa gatgtggagt tgggcaacga tccagaccgc agtccggttg cccgtgtacg
    10801 ggaactcaac cggcggcgcg ccaggagtct gcgcaaaggc cgctcccccc aacagcgcca
    10861 gaagcgcaca gcaagccaac aaaccgcgct gccatgtttt cataagtctc gccgcccctc
    10921 ccccgcgaat ccgattagga taatgcagtc gagtcgttac ttagccgcgc cagcttgggc
    10981 caccgccatg ttgcctgcgt ccgcccattc cttctttccg tcgtcccatt ttttcccgga
    11041 caatccgaaa ttgcgctcga ataggaccag cttccagcgg tcttcgtccg agagcttgct
    11101 cttccaggct ttcatcttgg tgttgggtac gccttcggaa atccgccaga accacacgga
    11161 atccgaatag agcttcatgc gctctccatt gcggaaatcg cgggcgccgg ccttaacagg
    11221 cttgccatcc ttgccatgac agctggcaca gttgacgtcc ggatttgttt cgccaatgaa
    11281 cagtttgcgg ccttcttccg ccttggccgc gtctccccac cagtcggcgg gcatgtgctt
    11341 gtcggcatat tccgcgggcg ccggaggtgg aggaaccacc ggcccttcgc cactttctcc
    11401 acacccgctc ataatcaacc ccacccccat cagcaatgcc tgcagcgttc tcgattgcgt
    11461 cataacggtt ttcactcctt ctatgatttc agacgaaaaa aaacgcttta acgggagtgc
    11521 ctggactcca gtaaagcgcc gacacctcaa gatcccggaa gctactcgca cggaccctcg
    11581 ccacatcctt gaccgatccg gcgaggtgcc atgagcccca cggaccgcca tcggtgttaa
    11641 tgctttctct tgctctgcga cttgcctcgt ttctgcccct gtttgcgccg tgccctggcg
    11701 atcttactga atccgacagc tccgattgca atggctgccg ttcctcctaa tccccaccac
    11761 acccacgtag gcgggctgtt cacatcgcgg acacagccgg ttccaaaatg aatgtgaact
    11821 ttacgctcag actccaggtc cttgggatcg aactggattg tatgattctg ctgttcaccc
    11881 ttggcctcga ccaggagcgc atcgccgaga ttttcattgt gcagatggaa cgtcgccttg
    11941 tagtgcccgt ctccgtcggt cttcaccacc tggccgtagg agatcttggt atctttgaca
    12001 aggacgtcgg tatcggccat tccctttcca tcagagccgc agacgaatcc ttcgacggtg
    12061 aaccgatggt cggcttcgtg cgtcgccgag ccgacggatg gaaaggcggt gaccagacac
    12121 acaacgaccg ctgcgaccct cagcctactc cgtcctggcc ctgcatccct caaactggaa
    12181 cgcgttcctg ccgctggaat ggtttcaaac aacaaaacgc ctcgccacgc caggcttccc
    12241 cagttttgag gcggatgttt ctagcatagg gccccctggt tgtcaacgag agggcccggc
    12301 cagacacacg accggagaaa gggaggagaa gcgggagaaa tcgtgctcag tcggaccggc
    12361 atctcgctgc tataaatgcc cgcgggtccg ggcgtctgct tctactcgag cggcttctgc
    12421 caggcggtcg gactgtttct tagagaccca ggtctcccac gatttcccat tggcggtgtc
    12481 aacgcccgta aatccaattg acagaatatc gcgataggcg atccgacggg agtcggaatc
    12541 ggccgcggag aggacttcga tccagggatc tgcaccggca tggtcacgat taaacacata
    12601 gcccgtcagc gtctccccgg atttgagctc gagggtcaca tcgcccctgt aatcaaacgc
    12661 cagttccacg gcttcggcgg tttcggcaga ggacgatggc cgaaagaccc gtccttccgg
    12721 tgaaccggca tctgccggcg tatgatcttt tgtatcggtc atagcctgcc tgtcgtcggt
    12781 gagatgttcg aaagttgaac gctgctagct cgagcgttat cgggaagtgg gcaggagggt
    12841 caactttgcc gttcgtgcga agcctgacag gcttccaaac gtatcctcga cggccgacgc
    12901 ctcatagccg caatgcacca tgcagtcagc gcatttctcg ttcttggtcg tgccgtagtc
    12961 ctgccattgg gtagtctcga tcagttcacg gaatgtcttg gcgtagccct cctgcaggag
    13021 atagcacggc ttttgccatc caaagacatt ataagtcggg ttcccccatg gcgtgcactg
    13081 atagtcgcgt cgtcccatca ggaaatccag aaagagcggc gattggttga actgccaacc
    13141 acgcctgggc tgactaagaa tctttgagaa aagctctttc gtcctggcgc gcttgaggaa
    13201 gtgttgctgg tccggcgcct tttggtagct gtagccgggc gaaatcatca tcccttcaac
    13261 gccgagcgcc atcatctcgt cgaaaaactt tctgacccgc tcgggattag cgtcatcaaa
    13321 cagcgtggtg ttcgtcgtga cccggtggcc gcgcttgagg gcggccctga tcgccctcac
    13381 ggcgacgtca tagacaccgt ctcggcagac ggccaagtca tgctcgtctt tcaagccgtc
    13441 catgtgaata ctaaacgtga ggtacttgga gggctgatac tcatcgagct tccgctcgag
    13501 caaaatggca ttggtacaga gatagacata gcgtttctgg gccacgagac ccttgacgat
    13561 cgtggccatc tcgggatgga tcaacggctc gccgccggga atactgacga tcggtgcgcc
    13621 gcattcccga gctgccgccc aacattgttc gggcgttaga cgtttatcga ggacgtggtc
    13681 agggtattgg atcttcccgc atccggcgca ggccaagttg caacggaaga gcggctccag
    13741 catgaggacc aagggatatc gcttcacccc tttgaacttc tgggtgagga catagctggc
    13801 aaccgtatac atttgtgaaa gcgggacagc catgacgaac tccaacgctt gagattagac
    13861 ggtcaccacg gcgagccgcg ggtgggcgaa ttgattgtaa acgctgcgga ttctaacact
    13921 ccatcatcga gtcgtcaatt tcgaagacgg ccgcgcctga agagccgccg gcacgcacca
    13981 agtgaagatt tagagcctac ttgtggcaaa gtacctctcc gctgcgatgt gatcgaacgc
    14041 agcggaacga gcaagctctg tttggaggcg ccgagcgggt tcgaaccgct gcatcgcagt
    14101 tttgcagact gctcccttag ccacttgggt acggcgcccc gggggaaccc attataagct
    14161 ggggatggga cgagtggcta gagcgaagac gcacgagcct gaaagcatcg tggcgtctcc
    14221 cgccggatgc gcatctacgc aatgaccaca ggtaatcaga agagtggtca gcgcttatta
    14281 gacagaacag ggatctcttt tcctgccgtt cggtcagact gaccgggcaa cgcttcccaa
    14341 ccttcggacg agggggagac cggttccccg gagctttcag cgtctttctc tttggcgagc
    14401 agttccacct cgtggatgag cgtgtccatt aattcttcca tgggcacttt ccggacgagt
    14461 ttacccttct tgaacagaat gcctttgcct tcaccacccg caatgccgat atcggcttct
    14521 ttgccttcgc caatgccgtt gacgacgcac ccgagcacgg agacattcag cgacgtcttg
    14581 atgtgactca gcttcttctc caattcattg gccattcgca cgacatcgat ttcgacacgg
    14641 ccgcaggtcg gacaggcaat cacattgatg ccgcgatggc gcagctccag cgatttgaga
    14701 atttcaaacc cgactttcac ttcttccacc ggatctgccg ccaacgacac acgcagcgtg
    14761 tcgccgattc cctgggagag caggtagcca aggccgatag acgatttcac ggcaccggtc
    14821 atcgccgtcc cggcctccgt gatgccgata tgcaacggat aattggattg atgggcgaac
    14881 agccagtagg catcgatcgc gtgatgcacg tcggacgcct tgagcgacac ctttagattg
    14941 gtaaacccca cgtcttccaa tgcgtgcacc gcattgagcg ccgattcggc aagcgcttcg
    15001 ggggacggcc agccatactt gtcgagcagc gggcgctcga gcgatccgcc gttcacaccg
    15061 acccgcagcg gaatgccacg tgcattcacc gccttaatga cctcttcgac cttccaccac
    15121 gcaccgatgt tgccgggatt gatacgaaca caatcaacga tctctgcggc tttcaaggcc
    15181 aaccgatggt cgaagtgaat gtcggcgatc aacggaacgg tcatggccgc cttgatcttg
    15241 ggaagcgcgg ccgccgcctc ctcgtccggg acggcgacgc ggatcagctc gcagcccgcg
    15301 ctctcgagct gtcggatctg ctcgaccgtc gccgggacgt ccctcgtatc ggtcgaacac
    15361 atcgactgga cagagatggc ggcatcgccg ccgatcttta ccgtgccgac ttgaatttgc
    15421 ctggtttgac gacgtgagat gtgcatcgta ccctgtcttc tggcctagct cccctgttca
    15481 tccccatgcg gcaggtgatc aatcgccgct gaaaagtatt ccgcctcgcg cgattcggcg
    15541 gggctcttgc tgaccagttc cttgacgcta cgggtgattc cgtccgtcgt caatccatag
    15601 cgttcgcgca agaggtcttg cggcccttgc tcgatgtacc aatcaggcag acccaggatc
    15661 ttcgttctga cgccggtcac accggcatcg gagagtgctt cgagcactgc cgagccgaaa
    15721 ccgcccatct tgcagccttc ttcgacggtt acgacgtaac ggacgcgctt ggccacctcg
    15781 acgatgagct cttgatccaa gggcttggca aagcgggcat tcaccaccgc cgtcgacaac
    15841 ccctcctgac tgagctgttc tgctgcctgt accgccggcc acacggacac gccaatggcg
    15901 atgatcgcga catcggtgcc atcgcgcagc agctcagctt tgcctatcgg cagtgctgag
    15961 agttcccggt ccatcgtcac acccaggctc actcctcgcg gataccgaac ggaggccgga
    16021 ccgtcatact gaagggcggt cttgagcata tgttgaagct cattttcatc cttgggcgcc
    16081 atgatgacca tgttgggcat gtggcgcaaa taggcataat caaatgcgcc atgatgcgtc
    16141 gtgccgtctt ccgccacgag cccgccgcgg tcaatgcaga acgtcactgg aagattttgg
    16201 gtggcaacgt cgtggacgac ttgatcgtag gcgcgctgca ggaatgtcgc atacatcgcc
    16261 acaaccggcc gcatgccctg cgcggccaag ccggctgcaa aggtcacggc atgctgctcg
    16321 gcgatgccga catcgtagag gcggtcaggg aattcctttt caaaggcatt caagccggtt
    16381 ccctcgcaca tcgctgcggt aatggccacg acccgacggt cctcccgggc caaccggatg
    16441 agcgagtcaa tggcgatctg agtataggaa ggtcgtacgg ctttctttgc tggagcacct
    16501 gtttcccgga caaaaggcgg acaggcatgg aaccacaccg gattcttttc ggccggcgca
    16561 taacccaagc cctttttcgt gattacgtga agcaggactg gtccccgcat cttgagcaca
    16621 ttttccagtg tcggaagcag atgctcgaag ttatgcccgt caatcggacc ggcatagcgg
    16681 aatcccaatt cttcgaacag caaccccggc agaatcgctc ccttggccaa ctcctctgcg
    16741 cggcgggcga acttttcgac gtcatagccg atatggggga tcttccgaag cagctgcccg
    16801 gtttcttccc gcatcttggt ataaaactct ccggtgaacg tccggttcag gtaggaagaa
    16861 atggcaccca cgttcttgga aatcgacatc tgattgtcgt tcaagatcac gagaaaatct
    16921 ttgccgaggc ctccggcatg gtgcaaccct tcgagggtca ttccggccgt catggcgccg
    16981 tcgccgacca cgcagaccac tttgtgcttc tgaccttgct gctccctcgc ctcgaccatc
    17041 ccatacgcgg cggagacgcc ggtgccggca tgacccgcat tgaaggtgtc gtagacgctc
    17101 tcttcacgct tgcagaaccc gctcagccct ccatattgcc gaagggtatg gaactgttcc
    17161 cgccggccgg tgagcagctt atgggcatac gcctggttgc tcgtgtccca gacaatcctg
    17221 tcctgtgggg tatcgaggag gtaatggagc gccacggtca attcaacaac cccgagattt
    17281 gaggcgagat ggccgcccac attggagacg gccccgatga tctgctctct aagctcttgg
    17341 cataatttag gaaattgcgc gggcttcagc cgcttgagat cagccgggct gtgaacggtt
    17401 tttaaaatcg acatggcaaa actcctttgc gagtgtcctg ccccagtatc agcgggccga
    17461 gttcagatag gcttggcgaa gagtgaagcg gttagcatac cggctccagc agtttcacac
    17521 aagacctcca tgaagtcaag cggttaggcc attttatccg ttggagtagg tcctttcccc
    17581 ttctgggaat gacgcccatc atgtgcggta ccggtaggtg aagaggactg ccacggtgaa
    17641 gtccgcggcc cctccggtaa tgaaatcgac gttttcgacg gcatacattt gccagagcca
    17701 ctgctcggct atgcggtagt tgaagccggc caccacctct gtggcgccct tgtcgaacac
    17761 ctcgagaccg acatgacgga aaggggtggt gtaatagtcg aactggaggg tcagtgacag
    17821 gttgtccgac catagatact cagtggccgc catcccggag atcgtcggat acaggggcaa
    17881 tccggcgata cggcccgtcg gcaggacagt gctcagattg ccgtacaaga tccaccgccc
    17941 tccgaggcgc ttctcggctg cgattccaat cccgaaatcc ggactgccgc ttccaaaaaa
    18001 ttcgcgttcg ttgcctgtgg ggagtttgac tgccgtccgg agcgacagcg ccggcatcga
    18061 ggacgtttcg ttcagcagct gatacttcgc ctggagtgtg ctgtcaccga gtccgaccgc
    18121 tcccttggtt ccactgacga tctgttgccc gttgcgtgaa atgctatagg cgtagctgac
    18181 gtggctgagc gcctggcgcg ccggggatat gccggtcgtc gctcgttcga cggcttcaat
    18241 cgcccccgac atgaaacccg tgtaccggta caacaccggc acttcgagag cgatctccag
    18301 cttacgggtc gcgccatacc gcaagaagag gccgttccgc agcgtttcaa attttacgct
    18361 ggctgtagcg cccggcgggg cttggctata gatggcagcg gtttcagcga gttcgaggcg
    18421 cacgtcccat gcgccgggct ccagtactgc cgcacggtca cccggcatgt tgaggaccag
    18481 ctgttggaga ggattgaaat tccgcacagg aaaggggccg aacccttcag cggaggctga
    18541 ggacgggtgc agacaggcca tgccgatgag gccagccaca agcgcccggc gcgttgaact
    18601 ccctcggcga tcgtcggagt aaagcatgtc agtatgggaa atccaggccg gcgaagatgg
    18661 cgcctccctc tttgctatat ccatagtcga ttcgaccgac cacattgggt cggacgattc
    18721 cccggaaccc gataccaggc gtcatgcggt aatccttgaa gctgacgtcc ttaaaatcgt
    18781 taaacacctg accggtgtcc aagaaggtcg ccatttcaaa atcagccgcc acgccggcga
    18841 tcttcgctct gacgagatga atacgctctt ccacgctggc cgcgattaaa tgcttgtcga
    18901 tgaagcgatc gacgccgtag ccgcggagat tgttttgccc tccaagcgaa ctctgttcaa
    18961 aaaacggcac ctgtgagccg agggttgcct gcaggtcggc ccggatgatc aggatggcgc
    19021 gcttggattc gctggggaag agcttcttca cttccaactc ataccgtgaa aacaccgggg
    19081 aatcactgtt gctcaggttc tgattgactt cggcatacgc cgtcacggcc atgccgtccg
    19141 tcggcgaaat gagattgttc ctcgtatcgt agtagaaggt tgcccggtgc ccgatgatga
    19201 tcgtttcccc ttgcacgccc tccaccgtgg ggtacagatc ccccgtaaac ggaagatcgg
    19261 tggcgcctct ctggaggctg acctggcgca cccgttgacc gacggcgatt tgcgtcacct
    19321 cattggcata gacgccgaaa cgccagttcg cccgcgcttc gcgcgccgtg taattggttt
    19381 catccgcctc gacggtggtt tgcccgagcc cgaaaaatcg agaggtcgca ttcttgaaga
    19441 acgtaccgcc gaaattcaag aagtaccgcc cgtcgctgaa ggccggatca atatagttga
    19501 agaccagctt tcgctcgatc ttctcggtca gcgacgcgat aaactgcatt tgccggccgc
    19561 ccgtgtcgta acgaaagaga ttgaatgtcc cacgcgtgcc gacgatcgag ttcttgatca
    19621 ccataggagc catcaaatac ttcaactctc cgtctggatc ggtgatgagg atggggacga
    19681 tcagccctgc gtcgctgccg tcatttttgg acgtggacgc ggacgggatc gggaagatct
    19741 gggtatccgc tgtggcgggc ctcacccaca cagcggtcat tcctacgacc aggcatacaa
    19801 aaaagatcgc gatgccgcgc agggtcgtca tggaaggatc ggctcgaagg tgttacgggg
    19861 ctttaatttt gtcggatttc ttgcgcagtt gctcgatcaa
//
LOCUS       DASSCQ010000099        14170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_63464, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000099 DASSCQ010000000
VERSION     DASSCQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63464"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..519
                     /locus_tag="VFH05_06960"
     CDS             223..519
                     /locus_tag="VFH05_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6677987.1"
                     /translation="MIRVVLPAHLRTLARVAGEVTVDVRGPVTQRSVLDAVEDGYPML
                     KGTIRDHVTKRRRAFVRFFACEQDLSHESPDALLPDAVATGAEPLLIVGAMAGG"
     gene            566..1816
                     /locus_tag="VFH05_06965"
     CDS             566..1816
                     /locus_tag="VFH05_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6677988.1"
                     /translation="MSEDAMDTAREAINEVYGAESRRVLATLIRLLGDFDLAEEALHE
                     AFAAAIEQWPRDGMPANPRAWLVSTGRFKAIDTMRRRARYDATLGEYAREYDSLVTES
                     AQADDQDIDDDRLRLIFTCCHPALSPEAQIPLTLREVCGLTTEEIAHAFLTKPATIAQ
                     RIVRAKAKIRDARIPYEVPAPSQLSERLDAVLRVIYLVFNEGYSASSGEAVTRAEVSG
                     EAIRLGRLLVELLPEVETRGLLALMLLQESRRAARTSADGDLVLLEDQDRLLWHQDLI
                     TEGLALVTQALSTQRIGPYSIQAAIAAVHAAAAEAAKTDWAQIVGLYDVLTRIEPSPI
                     VELNRAVAVAMRDGPLAGLVLIEGLFNRGELTGYHLAHAARADMCRRLGRHAEARTFY
                     KSALALTKQEPERRFLEQRLSSLE"
     gene            1950..2315
                     /locus_tag="VFH05_06970"
     CDS             1950..2315
                     /locus_tag="VFH05_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6677989.1"
                     /translation="MKYMLLVFHNERNFSELGEATHATMLDESIRLAHELASKGQYLA
                     APLQPTSTATCVRVRDHKQVVTDGPYAETHEQLAGYFLIDVKNLDEAITVAGRVPGAR
                     IGTVEIRPVRDVPGLPTVR"
     gene            2449..2904
                     /locus_tag="VFH05_06975"
     CDS             2449..2904
                     /locus_tag="VFH05_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6677990.1"
                     /translation="MRFMIIVKASKDSEAGVMPSEKLLAEMGKFNEELSNAGILLAGE
                     GLHASSKGARVKFSGSTRTVIDGPFAETKELIAGFWLWKVTSKEEAIEWVKRCPNPHN
                     EDSEIEIRQVFEAEDFGAEFTPELREQEDRVRAQAAKTASDRSASRDRR"
     gene            3005..3604
                     /locus_tag="VFH05_06980"
     CDS             3005..3604
                     /locus_tag="VFH05_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HET6677991.1"
                     /translation="MRFSVSILVALFVLSLASPLMAKDKKSEKPMDQQAMMELWKKMA
                     MPGEPHKLFATLAGSWTTTSKEWMEPGKPPTESTGTAEIKMLLDGRFLYQEYNSQMMG
                     QPFSGIGIDAYDNMTKKYVTAWLDSMGTGIFIMEGTASADGKTITLKGSHPEPGGGKM
                     THRAVWKIVDNNNQTFDMYGAHHGGKETKMMEIVYTRKP"
     gene            3663..4025
                     /locus_tag="VFH05_06985"
     CDS             3663..4025
                     /locus_tag="VFH05_06985"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HET6677992.1"
                     /translation="MAGYVDGYVLPIPKKHLQVYRRMAQKAGKIWREHGALDYKECAG
                     DDLNITMGVSFPRQMKVKSGETVVFAYILFKSRAHRDRVNAKVMKDPRLAGMGDPKDM
                     PFDHKRMVYGGFKVLVDA"
     gene            4244..4678
                     /locus_tag="VFH05_06990"
     CDS             4244..4678
                     /locus_tag="VFH05_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677993.1"
                     /translation="MPRTIGTLALMLMLLGLGGCSYFFYPRAGDYAAQAKGATTLDTM
                     INLTNMMEATAAKAKGGKGKDAALDDYHNQFHALLDSYCDVPKEQTKTPAYDMAVTHK
                     KELTAIFWRLWKFKDDQPQRDQHLDLSIAELKELRESLQAIK"
     gene            4704..5126
                     /locus_tag="VFH05_06995"
     CDS             4704..5126
                     /locus_tag="VFH05_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6677994.1"
                     /translation="MATNFGHIELITQNLDKAKDFYQKLFGWKARDLPDMPYSLFNEH
                     LFPTGGMMKSPQPGSESFWIPYIVTDDISGVTEKARSLGATIHREVMVAGQYGWLSII
                     NDPTGATFGLWQPKMERSTPRKKPTAKRTKTKSKRRRR"
     gene            5248..5730
                     /locus_tag="VFH05_07000"
     CDS             5248..5730
                     /locus_tag="VFH05_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6677995.1"
                     /translation="MKRLLVCTALFINMAVAVGSTPVAAESEAKSLWQLAPAQPDPPT
                     SPRKPWVLRERDITLDMQLLRILKDPTARPHPRVTLEFFDGHRHELDITSTVSRINDT
                     AVIRGTFKPPSQGEFTLVASGSLLVGSLQVGSRLYRTEHVANGRLRLVELDPSKAPPE
                     "
     gene            5793..6347
                     /locus_tag="VFH05_07005"
     CDS             5793..6347
                     /locus_tag="VFH05_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET6677996.1"
                     /translation="MSTISREPQLWTIGHSTKPIEDFLALLRSHGIRRLVDVRTIPRS
                     RHNPQYDTEVLAAHLKKAKLTYLHMPQLGGLKKAKKDSVNVGWKNASFRGYADYMQSE
                     QFQSALEQLMADSRETPTAVMCAEAVPWRCHRSLIADALLIRGWKVQHIMTKRKADSH
                     RLTRFAVVEADRITYPSASPSAKS"
     gene            6353..6790
                     /locus_tag="VFH05_07010"
     CDS             6353..6790
                     /locus_tag="VFH05_07010"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HET6677997.1"
                     /translation="MRYGLFADVIVLVHLLFVLFVMFGVLFALRWPGAIWMHAPALIW
                     GLIVEFADLTCPLTPLENRLRVAAGEAGSREDFLSQWLVTVLYPEFLTRDLQFFLGGS
                     LLFMNIGLYAYVLRKRSNRRPTKREAAYPSTGDPAGRHEGERL"
     gene            6885..7922
                     /gene="arsS"
                     /locus_tag="VFH05_07015"
     CDS             6885..7922
                     /gene="arsS"
                     /locus_tag="VFH05_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671339.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HET6677998.1"
                     /translation="MPSLLARRSPLAPTAAQLQLLAATTACEPFEVPMAQAGLRPLKA
                     TGITVLQLNVGKLCNQTCRHCHVDAAPDRTESMSRETAALCIEALAKSEIPTVDITGG
                     APELNQNFRWLVEQARRLQRHVMDRCNLSVLLLPAQADLAEFLAAHQVEIVASLPYYK
                     VQQTDAQRGDGVFKKSIEGLQRLNQLGYGRPNSGLVLNLVYNPVGAFLPPKQDAIESQ
                     FRKELHKHHGIEFNRLYTITNMPISRFLEFLVESGNYDGYMERLANAFNPAAAAGVMC
                     RYTLSVAWDGTLYDCDFNQMLNLPVDQRAPRHIRDFNPLALNDRSIVTGNHCYGCTAG
                     PGSSCGGSVTV"
     gene            8028..9062
                     /gene="hflK"
                     /locus_tag="VFH05_07020"
     CDS             8028..9062
                     /gene="hflK"
                     /locus_tag="VFH05_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET6677999.1"
                     /translation="MVWDPKDPWSRKSDPLEDALKQAQLQFKNLLPTGGFRNILLAGF
                     LLFLAWQSAFIIGPDEEGIVKRFGIPVRSVGPGPHLKIPLVESVLQPKVAKLHRVEIG
                     FRTDRQSRQQMIPQEALMLTGDMNILAIEFIVQYKINEARNFLFNVADIHDTIGKAAE
                     ASMREVIGKSKIDEALTTGKAQIQQDTQDLLQKILDQYQAGVQIAAVQLQDVDPPEAV
                     AAAFKDVTNAKEDREKLINQSIGYRNDILPKAKGEAAQVVNQAKGYAQARLNRAEGEA
                     NRFLATLREYNQARDTISKRIYIETMEEILPKMEKIIIDGKAAERLVPYLPIDRFSKK
                     SPVPTEGRQP"
     gene            9059..9919
                     /gene="hflC"
                     /locus_tag="VFH05_07025"
     CDS             9059..9919
                     /gene="hflC"
                     /locus_tag="VFH05_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET6678000.1"
                     /translation="MSKQGLTIAVIAVFIGLLILGASPLFVVDVTQTAIVVQLGKPVR
                     TVMEPGLYVKVPFFEEVTYFDKRLLDYDSDAQDVITQDKKTLLLDNFAKWLINDPLKV
                     YQNFQSQRGALQRLHDIIYSELRVELGRHDLAEIVSTARAELMKVVTQRANEKASAYG
                     ITIQDVRIKRADLPQENEKTVFQRMQAEREQQAKQYRAEGAEEAQKIRSDAEKDREII
                     LAQAYKESEELRGAGDAKAFRIYADAYRQDPKFFEFTRSMEAYKKSFDDKSTVVMTPD
                     SEFFRYLKQR"
     gene            complement(9923..11605)
                     /locus_tag="VFH05_07030"
     CDS             complement(9923..11605)
                     /locus_tag="VFH05_07030"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET6678001.1"
                     /translation="MTDLEIARAYRPLPIQDIAKRLGLSPDELHPYGRYKGKVALPAL
                     DRLKDKPKGKYILVTAINPTPLGEGKTTTSIGLAMGLCRVGRRAVVTLRQPSLGPVFG
                     IKGGGTGGGRAQVFPMEEINLHLTGDAHAVSASHNLLSAFIDNHLFHGNALGIDPARL
                     SWPRSLNVSDRALRHISLDESARRRPASFVITEASEVMAILALSSSQADLRRRLGQIV
                     VGVTTDGKPVRAEDLSCAGSMALLLKEALLPNLVQTLEGTPAFVHTGPFGNIAHGNCS
                     VISDAMALRCADYVVTEAGFGSDLGAEKFFNIKCRLSGFVPAAAVVVATLRALKLHGG
                     GGVVKAGSPVPVSLSGPNQEALTRGFANLEQHIANVKAHGIPVVVAVNAFKDDPPSEL
                     EWLRGQACKAGASEAAISTHYAEGGKGSEELARAVIRAADLPASFSCLYDQAWATKKK
                     IETIATKMYGASGVSFEPQAEEDMELATQLGYERLPVCMAKTPLSLSHDPALKGRPTG
                     FTLPIKEVRLLAGAGFLTAVCSGIQLMPGLPKKPAGERIGLDPQTGETVGLS"
     gene            complement(11732..12751)
                     /locus_tag="VFH05_07035"
     CDS             complement(11732..12751)
                     /locus_tag="VFH05_07035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6678002.1"
                     /translation="MGQVSGVESNDSYTVVIFRGSTAKPLRFSFPRKFVRKILILAAI
                     VLAADILVISHYVVRTGEVWELSAFRSEALSARQQTAAFSTAVDDLKKRLTSMKEVNQ
                     RLRVMLGIEMPKTGDQLNGRGGEETPLPEGSTVNSPDKLIRGGAGLQPASAVDGLIEL
                     NTSKSTDLDEQDLVAAVRDGIVWLAKEADVQEQTLQELALAAEQKSSRWAATPSIWPV
                     KGWVTSGFGPRVSPFTEKPAWHDGLDIGAAANAPVQAPAQGRVTSVGFDSKLGNVVKL
                     DHGFGIETLYGHLAKALVKEGQRVKRGDVVGLVGSSGLATGPHLHYMVKVNGQTFDPS
                     KYILD"
     gene            complement(12868..13203)
                     /gene="cutA"
                     /locus_tag="VFH05_07040"
     CDS             complement(12868..13203)
                     /gene="cutA"
                     /locus_tag="VFH05_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET6678003.1"
                     /translation="MRKSDVQILVILITTPNRREAVRIGQAVVKKKLAACANILSPVT
                     SIFRWKGRIEKSQEALLIMKTTSPRYAALAKTIGSMHPYEVPEIIALKVTKGLPRYIA
                     WVHQETAID"
     gene            13533..13886
                     /locus_tag="VFH05_07045"
     CDS             13533..13886
                     /locus_tag="VFH05_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6678004.1"
                     /translation="MMPERGKSEAIMGYTITPTELKSRLDKGDQLVLLDVREPWEFDL
                     AKLNGSTLIPLATLPQSLGKLNRETEIVAICHHGMRSADATNFLLQQGFSNVKNLVGG
                     IDAWSAQVDRSVPRY"
     gene            complement(13925..>14170)
                     /locus_tag="VFH05_07050"
     CDS             complement(13925..>14170)
                     /locus_tag="VFH05_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HET6678005.1"
                     /translation="ETQGTYNVGTGVETSINDLFRILVQHTGSVCKEIHGPAKKGEQA
                     RSVIDNTKLRQELSWEPRADLVDGLKNTVEYFQARVH"
BASE COUNT         3222 a         4077 c         3954 g         2917 t
ORIGIN      
        1 ctcgatccct gcggtgtgta cttcggcacc actggcgggc aggtctacgg atccgccgat
       61 ggcggcgaca catgggagcc tcttgtccgg gatctgccgg cggttctgtc agtggaagtc
      121 caggcactgt aacctgatcc acagatgtca gcatgcgcgg gaaagccgtt gaagagtccg
      181 gcgatgctgt tctttaatag gcaataaggg acagtgcaca cgatgatacg agtcgtcctc
      241 cccgcacact tgcgcacatt ggcgcgcgtc gccggggagg tcaccgtcga cgtccgtgga
      301 ccggtgacgc agcgctcagt tctggacgcg gttgaggacg gctatccgat gctgaaggga
      361 accatccgag atcacgtcac aaaacggcgg cgggcctttg tgaggttctt cgcgtgtgag
      421 caggaccttt ctcacgaatc gccggatgcc ctcctgcccg atgcggttgc cacaggcgca
      481 gagcctttgc tgatcgtcgg cgctatggcc ggcggctagt cgccggcctc aacaaaactt
      541 ctctcgaacc catgagccta ggcacatgag cgaagacgcg atggatacgg cgcgtgaggc
      601 gatcaatgaa gtgtatggtg cggagtcgcg ccgggtactc gccaccctga ttcggctcct
      661 tggcgatttt gacctggccg aggaagcctt gcatgaagcc ttcgctgcag cgatagagca
      721 atggccccgt gacggcatgc ctgccaaccc acgggcctgg ctcgtgtcga ccggccggtt
      781 taaggcgatc gatacgatgc ggcgtcgcgc gcgctacgat gccaccttag gcgaatatgc
      841 gagagagtac gatagccttg tcactgagag cgcgcaagcg gacgatcagg acatcgacga
      901 cgaccgctta cggctgatct ttacctgctg ccaccctgcc ctgtctcccg aagcgcaaat
      961 tcccctgacg ctccgtgaag tctgcggtct gaccacggaa gaaattgccc acgctttcct
     1021 caccaagccc gccaccattg ctcaaaggat cgtcagggca aaagcgaaga tacgcgacgc
     1081 tcggatacct tacgaagtac cggcgccatc ccaactctcc gaacggcttg atgccgtcct
     1141 gcgcgtcatc tacctcgtct ttaacgaggg gtattccgca tcctcagggg aagccgtgac
     1201 ccgcgcggag gtctcgggtg aagcgatccg cctcgggcgg ctccttgtcg agttgctgcc
     1261 cgaggtggaa acgagagggt tgctcgcgct catgctgtta caagaatcac gacgggccgc
     1321 gcgtacatcc gccgatggcg acctcgtttt attggaggat caggaccggt tgctctggca
     1381 ccaggatttg attaccgaag gactggcctt ggtcacacag gcgctctcga cgcaacgtat
     1441 cgggccttat tcgatccagg cggcgattgc cgccgtgcac gccgcagcgg ctgaggcggc
     1501 taagaccgat tgggctcaaa ttgtcggact ctatgacgtg ctcacacgca ttgagccctc
     1561 tccgatcgtg gaactgaata gggccgtcgc ggtggcgatg cgagatggtc cccttgccgg
     1621 tcttgtgctc attgagggcc tcttcaaccg aggggagctg acgggctatc acctggccca
     1681 cgctgcccgc gcagatatgt gccgtcgact cggacgacat gccgaagccc ggacctttta
     1741 caaaagcgcc ctcgcgctca ccaagcagga gccggaacgg cgcttcctgg aacagcggct
     1801 cagctcgtta gagtaaatac cgttgcatgg tgtggaggag gacgaactct caccaattta
     1861 cagggagttg cgagcaccgt cgatttttcc ggcccacgac gactatctta caagtcacgg
     1921 gtcaactgac gaaccgcgag ggggatcgca tgaaatacat gctgctcgtg ttccacaatg
     1981 aacggaactt ttccgaactg ggcgaagcga cacatgcgac catgctagat gagtcgatcc
     2041 ggctcgcaca tgagctcgct tcaaagggac agtatctcgc tgcgccgctg cagcccacct
     2101 caacagccac atgtgtcagg gtccgtgacc ataagcaggt ggtcacagac ggcccctatg
     2161 ccgaaacgca tgagcagctc gcgggatact ttctcatcga cgtgaaaaat ctggacgaag
     2221 ccatcacggt cgccggccgc gtgcctggcg cccgcatcgg cactgtggaa attcggcctg
     2281 tcagagacgt gcccggcctg ccaactgtcc gttagcacag aaggtcttgt tcgcgatgta
     2341 cttccgtagc aggttggtga cttggtcgat tttggcttct tcattcgact atcatgaaaa
     2401 ggccggtcga tggccgggaa aaaaattgac gggaaaggag tttccgctat gcgtttcatg
     2461 atcatcgtca aagccagcaa ggactccgaa gccggcgtga tgcctagtga aaagctgctg
     2521 gccgaaatgg gcaagtttaa cgaggagctg tcgaacgccg gtatcttgct cgctggggaa
     2581 ggccttcatg cgagttcaaa aggagcacgg gtaaagtttt cgggaagcac gcgcacggtc
     2641 attgacggac cgtttgccga gaccaaggag ttgatcgccg ggttctggtt gtggaaagtc
     2701 acgtccaaag aagaggcgat cgagtgggtc aaacgctgcc ccaacccgca caacgaggac
     2761 agcgagatcg agattcgcca ggtattcgag gcggaagatt tcggcgcgga atttaccccg
     2821 gaacttcgag aacaggaaga ccgcgttcgg gcgcaagccg ccaagacggc gtcagaccgc
     2881 tctgcttccc gagatcgtcg gtgaacagtc gacaagtatt ggcgaacctg aaaacagtga
     2941 gtgagcaaga gggctgatcg ccctcctcaa tttggaaacc atcgtctaac cccaggaggt
     3001 ctttatgcgt ttctcggtca gcattctcgt ggctcttttc gtgctttccc tcgcatctcc
     3061 gctcatggcc aaagacaaga aatcagaaaa gccgatggac cagcaggcca tgatggaact
     3121 ctggaagaag atggctatgc caggcgagcc gcataagcta ttcgcaacct tggcgggcag
     3181 ttggaccacc acaagcaagg aatggatgga accgggcaag ccgccgacgg aatccaccgg
     3241 gaccgccgag atcaagatgc tgctcgacgg gcgctttctc tatcaagaat acaattctca
     3301 gatgatgggc caacccttct caggcatcgg catcgacgcc tatgacaaca tgaccaaaaa
     3361 atatgtgacg gcctggttag attcgatggg cactgggatt ttcatcatgg aaggaacagc
     3421 aagtgcggac gggaagacca tcacgctgaa aggctcgcac cctgagccag gcggaggaaa
     3481 gatgacgcat cgtgcggtat ggaagattgt cgacaacaat aaccagacgt tcgatatgta
     3541 cggagcgcat catggcggca aggaaacgaa gatgatggaa atcgtgtaca cgcgaaagcc
     3601 atagcctaga cggacgttgg atatctcgcc gggggtttca acttgccgca aaggagattc
     3661 gcatggccgg atatgtcgat ggatacgtgc tgcccatccc gaaaaaacac ttgcaagtct
     3721 accgccgcat ggcgcaaaag gcgggaaaaa tatggcgaga gcacggcgcc ctggactaca
     3781 aggagtgcgc gggcgatgat ctcaatatta caatgggagt gtcattccct cgtcagatga
     3841 aagtgaaatc aggcgaaaca gtcgtgttcg cgtatatcct gtttaagtcg cgtgcgcacc
     3901 gagatcgcgt caacgccaag gtcatgaaag atccgcgcct cgccggcatg ggcgatccaa
     3961 aggacatgcc attcgatcac aagcgaatgg tgtacggtgg cttcaaggtg ttggtggatg
     4021 cgtgagcgca ccagacgaca gcactgatcg agctggccag caacacttcg cgtcctgtgg
     4081 catgtgatca gatggctcgc gcttggtgcg cattcctcaa taccggaacc cgccatgcgg
     4141 ttcacaagtc cctaccttgc ctcccattct cacgctcggc attgaccatc gccttgacaa
     4201 cgtgcttgac tagtcatcct cacttataca caggaggaac gatatgccgc gcacgatcgg
     4261 aacgctagcg ttgatgttga tgctgttggg actaggcgga tgcagctatt tcttttatcc
     4321 ccgcgcaggc gattatgccg cacaggcgaa aggcgcgacc accctcgaca cgatgatcaa
     4381 cctgaccaat atgatggagg caacagcagc caaagcgaag ggcgggaagg gcaaagacgc
     4441 tgcgctcgac gactaccata atcagttcca tgccctcctg gactcctatt gcgatgtacc
     4501 gaaagagcag acaaagactc ccgcctacga tatggccgta acccacaaga aggaattgac
     4561 cgccatcttc tggcgtcttt ggaagtttaa ggacgatcag ccgcagcgcg atcagcactt
     4621 ggatctctcg atcgctgaat tgaaggaatt gcgggaaagc ttgcaggcaa tcaagtaacc
     4681 ggcgagcgat cgacggaggc atcatggcga cgaacttcgg gcacatcgag ctgattaccc
     4741 agaacctcga caaagccaag gatttctatc agaagctgtt cggctggaaa gcccgcgatc
     4801 taccagacat gccatactcg ctctttaatg agcatctctt ccccaccggt ggcatgatga
     4861 agagcccgca acccggatca gaatccttct ggatccccta tatcgtgacg gatgacatca
     4921 gcggggtgac cgaaaaggcc cggagtctcg gcgccaccat ccatcgcgag gtcatggtgg
     4981 cgggacaata cggctggcta agcatcatca atgatcctac cggagccacg tttgggctct
     5041 ggcaacccaa aatggaacgg tccactccaa gaaaaaaacc aacggccaag cgcacgaaaa
     5101 ccaaatcgaa acgccggcgg cgctaggccc gggatagcca taacacccca gtctgcagat
     5161 ctctcgacgg tctcactggc cgcgccatgt aagaatgcca tcacgagccc ttcttcataa
     5221 cgggatgatg gttttcagga agattcaatg aagcgactgc tggtttgtac cgcattgttc
     5281 atcaatatgg ccgtcgctgt cgggtccaca ccggtcgctg ccgagtcgga ggccaagagt
     5341 ctctggcaac tggcgccggc acagccggat ccgccgacat ccccacgaaa gccatgggtg
     5401 ctccgtgagc gagacatcac gctggacatg cagcttcttc gaatattgaa agatccaaca
     5461 gcaagaccgc acccgcgcgt gactctcgaa ttcttcgacg gacatcgcca tgagttggac
     5521 atcacctcga ccgtgtcacg catcaacgat actgcggtca tccgcgggac gtttaaaccc
     5581 ccttctcaag gcgagtttac gttggtggcg agcggcagtc tgctggtggg cagcctacaa
     5641 gtgggaagtc gcttgtatag aactgagcat gtggccaacg gcaggctcag acttgtggaa
     5701 ctggacccta gtaaggctcc gcccgaatag cgccctccac tcatcttgat ccggcctgaa
     5761 ttcttcatcc agaatggaga ggggtacagg ccatgtcgac catatcacgc gaaccgcagc
     5821 tctggaccat cggccattcg acgaaaccca tcgaagactt cctggccttg ctgagatccc
     5881 acggcattcg acgtctcgtt gatgtgcgaa cgattccccg ttcgcgacac aatccgcaat
     5941 atgataccga ggtactagcg gcgcatctga aaaaggcgaa gctgacctac cttcacatgc
     6001 ctcagctcgg cggtttgaag aaggccaaga aagattcggt gaacgttggc tggaagaacg
     6061 ccagctttcg aggatatgcc gactacatgc agtcggaaca gttccagagt gccctcgagc
     6121 aactgatggc cgacagtcgc gaaaccccga ccgccgtcat gtgcgcggaa gcggtgccat
     6181 ggcggtgtca ccgatcgttg atcgccgatg ccctgctcat ccggggatgg aaggttcagc
     6241 acatcatgac gaagaggaag gctgacagcc atcggctgac gcgatttgcc gttgtcgaag
     6301 cagatcgcat cacgtatcca tctgcctcac cttccgccaa gtcgtgaagc gtatgagata
     6361 tggccttttc gccgatgtca tcgtgctcgt gcatcttctc tttgtgctgt ttgtgatgtt
     6421 tggtgtgcta ttcgcactcc gatggcccgg cgctatctgg atgcatgctc cggcgcttat
     6481 ctggggactc atcgtcgagt ttgccgatct cacctgtcca ctcaccccgc tggaaaaccg
     6541 gctacgtgtg gcagccggag aagccggcag cagggaagat ttcctctcgc agtggcttgt
     6601 gactgtcctc tatcctgaat ttctgactcg ggatctccag ttctttcttg gtggatcgct
     6661 cctgttcatg aatattggcc tctatgccta cgtgttgagg aagagatcga accggcgacc
     6721 aacaaagaga gaagcagcct atccctccac cggtgatccg gctggacggc acgaaggtga
     6781 gaggctctga cggttgttga cctgcacgtg aatctctggc tagatgagta aggcgaactg
     6841 cgtctggaca ggcatctttt agaccgcatt gacagagcca gctcatgcca tcactccttg
     6901 cacgaagaag tcctctagct ccgacggcag cgcagctgca gcttctcgcg gccaccaccg
     6961 cttgcgagcc gtttgaggtc ccgatggcgc aggccggcct gcgcccgctc aaagcgaccg
     7021 gcattacggt ccttcagctc aatgtaggga aactgtgcaa tcagacctgc aggcattgtc
     7081 acgtagacgc ggcaccggac cgtacggaga gcatgtcccg tgagacagca gcactctgca
     7141 tcgaagcatt ggccaaaagc gagattccta ccgtcgacat caccggcgga gctccagaac
     7201 tcaaccagaa ttttcgctgg ctggtcgaac aggcgcggag gctacagcga catgtgatgg
     7261 accgctgcaa cctttcggtt ctcctgttgc cggctcaggc cgatttggcc gaattcctgg
     7321 cggctcatca ggttgagatc gtagcgtccc ttccctatta caaagtgcaa cagacagatg
     7381 cccagcgcgg agacggcgtc ttcaagaagt cgatcgaggg gctccagcga ctgaatcaac
     7441 tcggctatgg acgacccaat agcggcttgg tattgaatct cgtgtacaat ccggtcgggg
     7501 cgtttttgcc tccaaaacag gacgcgattg aatcgcagtt tcgcaaagag ctacacaagc
     7561 atcatggtat tgagttcaat cgtctctata cgatcacgaa catgccgatc agccgcttct
     7621 tggaatttct tgttgagagt ggaaactatg atggctacat ggagcgcctc gcgaacgcct
     7681 ttaatccagc tgctgcagca ggcgtcatgt gccgctatac gctatccgtc gcgtgggacg
     7741 gcacgctgta cgattgcgat tttaaccaga tgttgaacct ccccgtcgat caacgggcgc
     7801 ccagacacat acgagatttc aatcctctgg cgctgaatga ccgatcaatc gtcacaggga
     7861 atcattgtta cggttgcacc gccggccccg gctcgtcgtg cggaggctct gtgaccgtct
     7921 agccgcgctg caccccgctc cgtcagccct cagcgtgttg cgaaggcacg ggcactctgc
     7981 tatggtgtcg cccgttctca agcagtaaac cttccgcaaa tcgccgcatg gtctgggatc
     8041 ccaaagaccc gtggagcagg aaaagcgacc cgcttgagga tgcgctcaag caggcgcaac
     8101 tgcaattcaa gaacctgctt cccaccggcg gattcagaaa catcctgctg gctggattcc
     8161 ttctctttct agcctggcaa agcgcgttta tcatcggacc ggacgaagaa ggcattgtga
     8221 agcgcttcgg cattccggtg cggtcagtgg gtcctggccc tcatctcaag attccgctcg
     8281 tcgagtcggt gctacagcca aaagtcgcga aacttcaccg cgtggaaatc ggctttcgga
     8341 cggatcgcca aagccggcaa cagatgattc cacaggaagc cctgatgctc accggtgata
     8401 tgaacattct ggcgatcgag ttcatcgtcc agtacaagat caacgaagcc cggaacttcc
     8461 tattcaacgt tgccgacatc catgacacca tcggcaaggc ggctgaagcg tccatgcgcg
     8521 aagtcatcgg caagagcaag atcgatgagg ccctgacaac ggggaaagcg cagattcagc
     8581 aagacacaca ggatctgctt cagaagattt tggatcagta tcaggcgggt gtgcagatcg
     8641 ccgccgtgca actccaggat gtggaccccc ctgaagccgt ggccgccgcc tttaaagatg
     8701 tcaccaatgc gaaggaggac cgtgaaaagc tgatcaatca atcgatcggc tatcgcaatg
     8761 acatcctgcc gaaagcgaaa ggcgaagcgg cccaggtggt gaatcaggcc aagggctatg
     8821 cacaagcgcg cttgaatcgc gccgagggcg aagccaatcg gttcctggcc accctgcggg
     8881 aatacaatca agcgcgggat accattagca aacgtattta catcgagacc atggaagaaa
     8941 ttttgccgaa gatggaaaag attatcatcg atggaaaggc cgccgagcgg ttggtcccct
     9001 acttacccat tgaccgcttc tccaaaaagt cccccgtacc gacggaggga cgccagccat
     9061 gagcaagcag gggctgacca ttgcggtcat cgccgtcttt attggtctct tgatccttgg
     9121 cgcctccccg ctgtttgtgg ttgatgtcac ccagacggcc atcgtcgtgc aactgggcaa
     9181 gccggttcgc accgtcatgg aacccggcct gtacgtgaag gtgccttttt ttgaagaagt
     9241 cacctacttc gacaagcgcc ttctggatta cgattcggac gcccaagatg tcatcacgca
     9301 ggacaagaaa acgttgctgc tcgacaactt tgcgaagtgg ctgattaacg atccactgaa
     9361 ggtctaccag aacttccaaa gtcagcgcgg ggcactgcag cggcttcacg acatcattta
     9421 ttcagagctg cgggtggaat tgggccgaca tgatctagcc gagatcgtct ccacggcgag
     9481 agccgagctg atgaaggtcg tcacccagcg ggccaacgaa aaggcctcgg cttatggcat
     9541 cacgattcaa gacgtacgga tcaagcgggc ggatctgccg caagaaaatg aaaagacggt
     9601 tttccaacgg atgcaggcgg aacgggagca acaagccaag caatatcgtg ctgaaggtgc
     9661 ggaggaagcc caaaaaattc gatcggacgc ggaaaaggat cgtgaaatca ttctggcgca
     9721 agcctataag gaatcggaag agcttcgcgg cgccggcgac gcgaaagcct ttcgaattta
     9781 tgccgacgcc taccgacagg atcctaagtt tttcgagttt accaggtcca tggaagcgta
     9841 caaaaagagc ttcgatgaca aatccacggt tgtcatgacc ccggactctg agttcttccg
     9901 ctatctcaaa cagcgctgat cgtcaggaga gcccgaccgt ttcgccggtt tgcggatcga
     9961 ggccaatccg ttctccggcc ggtttcttcg gcaaccccgg catcaactgg atgccggagc
    10021 agacggccgt gagaaaccca gcgcctgcga gtaatcgcac ctctttgatc ggcaaggtaa
    10081 acccggtggg acgacctttc agcgcggggt cgtgcgaaag tgacagcggc gtcttggcca
    10141 tacacacagg cagccgctcg tatcccaact gcgtggccag ttccatgtcc tcttcagcct
    10201 gcggctcgaa tgagacccct gacgcgccgt acatctttgt ggcaatagtt tcgatcttct
    10261 tctttgtcgc ccaagcttga tcatataagc aggaaaatga tgcgggcagg tcggcggcgc
    10321 ggatcaccgc ccgcgcgagt tcttccgacc cttttccgcc ttccgcgtaa tgggtggaaa
    10381 tcgccgcttc tgacgcgccg gccttacatg cctgtcctcg cagccattcc aattctgaag
    10441 gcggatcgtc cttgaacgcg ttgacggcca cgacgaccgg aataccgtgg gccttgacgt
    10501 tggcaatatg ctgttccaga tttgcgaatc cccgagtgag cgcttcctgg ttgggcccgg
    10561 agaggctgac cgggacgggg gagcctgctt tcaccacgcc tcccccacca tggagtttga
    10621 gcgcgcgtaa cgtcgcgacg accacggccg ccgccgggac gaaccccgac agtcggcact
    10681 tgatgttgaa gaatttctca gcgcccaggt cgcttccaaa cccggcttct gtgaccacgt
    10741 aatcggcgca gcgcaaagcc atggcgtcag agatgaccga acaattacca tgcgcgatat
    10801 tgccaaacgg accggtgtgg acgaatgccg gtgtcccttc gagcgtctga accaaattag
    10861 gcagtagagc ctccttcagg agcaaggcca tggagcccgc acacgacaag tcctccgctc
    10921 gaaccggttt tccgtccgtc gtcaccccaa ccacaatttg gccaagccgg cgtctcaagt
    10981 cagcctgact cgatgacagc gccaggatgg ccatgacctc cgatgcttca gtgatcacaa
    11041 agctcgccgg acgacgccgg gcgctttcgt cgagcgagat gtggcggagt gcccggtcgc
    11101 tcacattcaa cgatcgtggc cagctcaggc gagccggatc gatgccgagt gcgttgccat
    11161 ggaacaggtg gttgtcgatg aacgccgaca acaaattgtg actggctgaa acggcgtgag
    11221 catcccccgt caggtgcagg ttaatctctt ccatcgggaa gacttgagcg cggccaccgc
    11281 cggttccgcc tccttttatg ccgaacaccg gtccaagcga tggttgacgc aaggtcacca
    11341 cagcacgcct accgactcga cagagtccca tcgccaatcc gatcgaagtc gtcgttttcc
    11401 cttctcccag cggcgtcgga ttgatcgccg tcaccaagat gtattttccc ttcggcttgt
    11461 ctttcagccg atccagcgcc ggaagagcca cctttccctt gtatcgtcca tagggatgca
    11521 attcgtccgg cgagaggccg agtcgtttgg caatgtcctg gatggggaga ggccggtagg
    11581 ctctcgcgat ctcgagatcg gtcatgggtt tggtgagatg gaaggtaagg ccagtgccat
    11641 gtagaccacg ccatgccagc gcagaccgga caaaaaaata cgcgctatgt cagaacgact
    11701 agcgcgtaat caggctcgct ggaagaacca tttagtcgag aatgtatttc gaaggatcaa
    11761 atgtctggcc gttcaccttg accatgtaat gaagatgggg gccggtggcg aggccgctgc
    11821 ttccaaccaa tcccaccacg tcaccgcgct tcacacgctg cccttccttc acaagagcct
    11881 tggccagatg tccgtagagc gtctcaatac caaatccatg atcgagcttg accacgtttc
    11941 ccagcttcga atcaaacccg acgctcgtga cacggccctg tgcgggcgcc tgcaccggcg
    12001 cattggcggc agctccgatg tccagcccat cgtgccacgc cggcttttcg gtaaatggtg
    12061 acacgcgcgg cccgaatccc gaagtcaccc agcctttgac cggccaaatg gaaggagtgg
    12121 cagcccaacg ggaggatttc tgttcggcag cgagggcaag ttcctgaagg gtctgttctt
    12181 gcacatccgc ttctttggca agccaaacga taccatccct gacagcagcc acgaggtctt
    12241 gttcgtcaag atccgttgac ttggaggtgt tcagctcaat caggccatcg acggcgctcg
    12301 ctggctgaag ccctgcaccg ccgcgaatga gcttgtcagg ggagttcacc gtactgccct
    12361 cgggaagtgg ggtttcttca cccccacgcc cgtttaactg gtcacccgtc ttcggcatct
    12421 caatgcccaa catgacccga aggcgttggt tgacctcctt catactggtc agccgctttt
    12481 tcaagtcatc gaccgccgtc gagaaggccg cagtctgctg gcgagcgctg agcgcttcgc
    12541 tacggaaagc cgataactcc cagacttcac ccgttctgac cacgtaatgg gagatcacta
    12601 ggatatcggc cgcaagtaca attgcagcta atatcaatat cttacgaacg aatttccgtg
    12661 gaaagctaaa ccggagtggt ttggcggtgg aaccccggaa aatgaccacc gtataagaat
    12721 cgttggactc aacgccgctc acttgtccca tatccctcac cccctgctgc aagggtgtcc
    12781 ctccgcatgc ccaattctga tttcgagtgt acccatccag gcagcgttgg tcaacccctc
    12841 aatttggtaa agaagcctgt aatcaactca atcaatagcg gtttcctgat ggacccacgc
    12901 tatatatcga ggtaaacctt ttgtcacttt caaggcgatg atttctggca cttcgtatgg
    12961 atgcatcgag cctattgttt ttgccagggc agcgtaccta gggctcgtcg ttttcatgat
    13021 cagcagggcc tcctggctct tctcgatgcg gcctttccac ctaaagattg atgtcaccgg
    13081 cgaaaggatg ttcgcacagg ctgccaattt cttcttcacg actgcttgtc cgatacgtac
    13141 cgcttctcgc cgattgggcg tcgtgatcaa gatgacaagt atctgcacat ctgacttcct
    13201 catggcccac cctcccacga atcccaattg gtggcaaggg ccggcgaggc cgttcagtcc
    13261 cttgcttgag caagtaccgt gccctaccta gagacaaaaa agatacatga ctaaacattt
    13321 caatacctta gatcgtcctg gcgcgctagg atggccaagg aaagactcgt aggagccgac
    13381 tggcggtgca gttatccgta acaaaaatga acaattattg tcctcaggtg aactgcgtat
    13441 cgcatccttt cgaacagcat tgcgagcagt gagatgcctc gggcgggtcg tgatccgggt
    13501 gactggattg acagcctttt cgaggctcac ttatgatgcc tgaaagagga aaatcggagg
    13561 cgatcatggg ttacaccatc actcccactg aattgaaaag ccggctcgat aaaggcgatc
    13621 agctcgtcct cttggacgtc cgggaaccat gggaattcga tctggccaag ctgaacgggt
    13681 cgacgctcat ccctctggca acgcttcccc aatcgctggg taaactcaac cgcgaaaccg
    13741 agatcgttgc gatttgccat catggaatga gaagtgccga cgcgaccaat ttcctcctcc
    13801 aacaaggatt ctccaatgtg aagaacctgg tgggtgggat cgatgcttgg tcagcgcaag
    13861 tggaccgatc agtcccgaga tattagtgcc cgtccacctg cgtgatgcca gagacgcgcc
    13921 attgtcaatg tacgcgcgcc tggaaatact cgaccgtatt cttcaggcca tcgaccagat
    13981 cggctctcgg ctcccaggaa agttcctgac gcagttttgt gttgtcgatg acactgcggg
    14041 cctgctcacc cttcttggca ggaccgtgaa tctccttgca aaccgagccc gtatgctgga
    14101 ctaagatcct aaaaagatca ttgatggacg tttcgacgcc cgtaccgacg ttatatgtgc
    14161 cctgcgtttc
//
LOCUS       DASSCQ010000100        28652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1725154, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000100 DASSCQ010000000
VERSION     DASSCQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 28652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28652
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725154"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..481
                     /gene="rplQ"
                     /locus_tag="VFH05_07055"
     CDS             65..481
                     /gene="rplQ"
                     /locus_tag="VFH05_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET6678006.1"
                     /translation="MRHRKKGRQLGRQTKHRWALFRSLVTSLLEHERIETTEAKAKEI
                     RGFTDRMITLGKDGSLPARRRALSFLRSKDVVSKLFSDVAGRFKDRPGGYTRMVKTRR
                     RIGDAAELVAIELVARAEVSTSKKPGSTPAKTDKTE"
     gene            660..1232
                     /gene="lptC"
                     /locus_tag="VFH05_07060"
     CDS             660..1232
                     /gene="lptC"
                     /locus_tag="VFH05_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET6678007.1"
                     /translation="MWQRWAQRILLGLSLALVCFLVYLLMTNSTASPPPTATAPGTIN
                     QADAIISKFTFTQTKGDKVQWQVDAQQARLYEQDKRAILDNVAVTLFGVQGKEMTVHG
                     DEGTLNTDTKNFLLTNRSEPLVIYTQSGYTIYTNHLAWTDQTREIRTQDPVHIIGHGL
                     DVKGRGLLGHLDTEEFEVLEDVHVDVAPAS"
     gene            1243..1773
                     /locus_tag="VFH05_07065"
     CDS             1243..1773
                     /locus_tag="VFH05_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HET6678008.1"
                     /translation="MSMPTVGQAAMGAGSNGASDEALGTTVISQRMTFKNQQSQAVFD
                     GAVVLTRGTLVVYSDRMVVSFRNQEASDSKKASEPPKGGPAPAKGPDTVSNRAVSQIE
                     ATGRVKIERDAGNATCQKAVYYRDEDKIVLTGDPVAWDKGTRVSGKRITMFLAEDRTV
                     VEGGSHVRIEPEGGTK"
     gene            1770..2552
                     /gene="lptB"
                     /locus_tag="VFH05_07070"
     CDS             1770..2552
                     /gene="lptB"
                     /locus_tag="VFH05_07070"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET6678009.1"
                     /translation="MTEAVRLAPETDGVARSSEPRAVLRADGLVKSFRARKVVKGVSV
                     EVSAGEVVGLLGPNGAGKTTIFDMIVGLTQPDEGSIRLNAEEITALPMYKRARNGIGY
                     LPQESSVFRRLSVQDNILAILEMLDYSRAERAERVDVLLGELDLGHIRTSMAYALSGG
                     ERRRLEITRALATNPTFMLLDEPFAGIDPIAVADIQQIITRLKGKGIGILITDHNVQE
                     TLSITDRAYIINEGLVLESGPPDVIVKSETARAVYLGERFTL"
     gene            2568..4088
                     /gene="rpoN"
                     /locus_tag="VFH05_07075"
     CDS             2568..4088
                     /gene="rpoN"
                     /locus_tag="VFH05_07075"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET6678010.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLAQHLMEN
                     PLLDELPEEVEENESTTAEEKAEEAAASASPANETSETDESSTPEERDTPDEVSAAGW
                     EEYFGSDRRTGGSEGQSSSSDDFPSYEQTVAKAASLEDHLLWQLSLSGLSERNKAIGR
                     LLIGNLDDDGYLRTPLSEIVTGTDFTEAEAESVLRDIQTFDPTGVGARDLPECLMLQL
                     GHLGKSPMGSLGARPGALKGSIVESVVLHHLKDLEKKQYAKVAKALDVTVEEIFQATK
                     VIEALEPKPGRPFSNTQNYVIVPDVFVVKNEGEWVVLLNDDGLPRMRISPYYKQMMAS
                     GEGGSTETKAYLDEKLRAAQWVIRSIEQRNKTIVKVVSSIVKFQEQFFEKGIQYLKPL
                     VLKQVAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADQPSDMLSSVTVR
                     EMIKKMVAEEDANRPLKDEEIAARLRERQVLIARRTVAKYRAEENIPPATQRKRFF"
     gene            4137..4640
                     /gene="raiA"
                     /locus_tag="VFH05_07080"
     CDS             4137..4640
                     /gene="raiA"
                     /locus_tag="VFH05_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6678011.1"
                     /translation="MKITGRHMDVTSALKRYIKSRFERLERYDVSPDRLEIVLSVTKH
                     QHTAEVVCSIRGKRFQAKTSTTEMYATVDQLVDRLDKQIRKHKERRIAHKGRTKTQLP
                     ATVGASPDLNDLKIVRPKIAVLSRKEARDRLDADPGAMLLFTCVDSGKLQILRRSETG
                     HVVLIDP"
     gene            4650..5516
                     /gene="rapZ"
                     /locus_tag="VFH05_07085"
     CDS             4650..5516
                     /gene="rapZ"
                     /locus_tag="VFH05_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET6678012.1"
                     /translation="MPGLKLVVITGLSGAGKSHALKCFEDVGYFCVDNLPPALLPTFV
                     ELCHQQGGEIKNVALGIDIRERVFFADLTGILQRIKDLGHSVELLFLEARDPVLVRRF
                     SESRRPHPLLPELPVLEGVRFEKERLAEIRAHADRIIDTSNLSVHDLRDLLSRHFSQE
                     LSARRLTISLITFGYKFGVPYDIDLLFDVRFLRNPYFVPDLKPLTGEDPRVRSYVLDD
                     PDAKGFLDRLEQFFTYLIPLFERERRSYLNVGIGCTGGRHRSVVIAKRLHESFSSAGH
                     HVTLSHRDLTKP"
     gene            5763..6875
                     /gene="pilM"
                     /locus_tag="VFH05_07090"
     CDS             5763..6875
                     /gene="pilM"
                     /locus_tag="VFH05_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET6678013.1"
                     /translation="MLNSLKSLVDKDIFSLLTPKRQLVGLDIGSSGIKLVQLKENRGR
                     YVLQKFGFKPLEPEVIVDGTVMDEGQVVSAIKALFDELNVKVKQVAVSISGHAVIIKK
                     ISLPPMPDDELEGQVKLAAEQYIPFDINEVNIDFHVLPPSEGDGSGEAEMSVILVAAK
                     KDKINELTELVKGAGLLPLVMDVDAFAIENMHAINYPMSQEETTALVNVGASVMNINI
                     VSKGTSLFTRDIPIGGNRYTESIQRELGLSYEEAEQLKKGGKASGNNQAAAAQSVIES
                     VNAEVASEIARTIDYYKSTAAEGEVQQVLLCGGGAQVSGLVKQLRDRMHAVVEVANPF
                     AEVDTSGSDFDQTTLAEMASMASVGVGLALRSVGDR"
     gene            6872..7426
                     /locus_tag="VFH05_07095"
     CDS             6872..7426
                     /locus_tag="VFH05_07095"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET6678014.1"
                     /translation="MIRINLLSAGPKGRQAKPQYDVRAQVLLGVGLLVITLSACWWYS
                     ASLDDEIATRQTEKSEKEKQVAQLKEQVKQVSDFELRKKQLEDKNRIIDQLEKSRVGP
                     VKVMDHVSHSLEPLKLWLVKVGVTGQSIELEGRALTNDDVVEFVNNLRRTDYFSDINL
                     QESRAAVESQISLFQFKLGFRLKG"
     gene            7432..8052
                     /gene="pilO"
                     /locus_tag="VFH05_07100"
     CDS             7432..8052
                     /gene="pilO"
                     /locus_tag="VFH05_07100"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET6678015.1"
                     /translation="MNLPAMNLEAIRTIPLMQKVAFMVLVLAGIVVMFYYYVFEPKSM
                     EIAALEGEVGKLDTEIQTLTIKVKHLDELIAASKQLEIELAKKKERLPPEEEAVMLLK
                     QVSDLGIKLGLDIKLWKPGAKSEDPSKLFVRLPVNVEVTGGYHTAALFFDRINGLPRI
                     VTVSNLKMGMPKTEKGRVVTQTVFDLVAYAAPQDVKVAAAPAPAPK"
     gene            8081..8677
                     /locus_tag="VFH05_07105"
     CDS             8081..8677
                     /locus_tag="VFH05_07105"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HET6678016.1"
                     /translation="MVKIGGLGTLFGCMLLLAMMGRSLAEPGLASHLGQVAPLRQDSL
                     KVPSPNDLTKEPPQSALTLSPDLAAAPTDALPGAPPFPIGGYDPAGRRDPFSPVLSQL
                     APGQTDPTLPPLQRIGLTDLNLIAIIWGAYGYTAMVQTPDGNGYTVRKGTRVGPNSGV
                     VSAVTEKGIIVQERFTDVYGRKQEREYVKLLHPKEGLE"
     gene            8674..10641
                     /gene="pilQ"
                     /locus_tag="VFH05_07110"
     CDS             8674..10641
                     /gene="pilQ"
                     /locus_tag="VFH05_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HET6678017.1"
                     /translation="MTHIQRHASTTVRILSALAAVSLPMAIPSTGASADVNAGVSMNR
                     PGIDQGASDRRLASAVTGIDVKKEADAVSVTISGDGSLLHEALRVDERRLVVDIPRVS
                     SEIRRPTLNVGHRLVKQIRIGDHSDKVRIVFDLGQQAEYAVEPQGASLLVRVHESIGG
                     GSMPEQDSTNAHTSAAPSAAADIPLIESAQGGRHRVQPVVHRTTPGFRVRPIQMMAEP
                     DAVEKHTPKDDLVVGETRYVGRRISLDFQQADISNVLRLIAEVSGFNIVIGDGVKSKV
                     TMKLVSVPWDQALDMILKMNGLGKIKQGNILWIDTLSNIAKQEDEEARAKDAKVKAED
                     LVDRVFYIRNLQATELMTSLRQNLSPRGLMQISTGTNALVVRDTETKMAVLKQLIDGL
                     DLEVPQVQIEARIVQADTVYARGLGVQWGIANADFGTNTFSAVGNLTGAFAPAAGTGA
                     SVIPRDFLVNLPAQVAGLPSVPGIGYSFGKLAPGFALDMRLSAGELLGLAKVIAAPKV
                     TTLDKREAKISQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEVGKQIMMK
                     VRATRNAVGARSNPAGPSIDRREANTQVIVRDGETMVIGGVFIDTQNNNVQGVPYLSR
                     IPVLGWLFKNKSESVSKQELLIFLTPSIVKA"
     gene            10824..11951
                     /gene="aroB"
                     /locus_tag="VFH05_07115"
     CDS             10824..11951
                     /gene="aroB"
                     /locus_tag="VFH05_07115"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET6678018.1"
                     /translation="MIGQHVPVRLGERSYDIVVEPGLSRSLGALLKPLMDTGKVGIVT
                     DRHVARHYLKPTIESLKQAGYEPVTIVLPPGERSKTLTTIARILDVLAKQRFERRSLL
                     LALGGGVIGDLTGFAAAIYQRGIAYVQVPTTLVAQVDSSVGGKTGVDHRLGKNLIGAF
                     HQPRAVFIDPFTLHTLPRREWIAGLAEVIKYGVIADEVFFSRLEASMSDLLKRNAHAV
                     MQAVTRSCQIKAEVVAADERESDRRRILNYGHTIGHALEVLCNYRNLIHGEAVAIGMV
                     AEADLSVHLGLCKQDVSARIRGLVQAAGLPDRMPDTSFAALWRAMQHDKKVVGGKVMG
                     VWPIRLGEVVMRPLESEACADWHATLSGSNSARQKRRIRRA"
     gene            11955..13217
                     /locus_tag="VFH05_07120"
     CDS             11955..13217
                     /locus_tag="VFH05_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HET6678019.1"
                     /translation="MAKRPPSLTQLEQALREKTREVDVLHRITDSISNTLDLEAVLKH
                     IVEIVVEVTKADACLLYLLSDGEDELILRASKNPHPKLIGRITIGMGEGITGWVARER
                     TRVVIPSNASDDPRFKFFHNLPEDRHQAFVSVPIMAKKEVVGVINVQHKRPKRYRPDE
                     LALLSTIANQVGGAIENARLYEQMRRKALQVETLSQVSETVASNRLIEDVMQLLVTMT
                     AQMMNSKICSIMLLDETTGDLRIEATQSLSEHYRRKPNLKVGQSVSGRAVQERRPIMV
                     SDVTKEQDYMYPDMAKKEGLCSLLSVPMLVREKAVGVINSYTSVPHTFTAEEVKLLQA
                     IANQAAIAIEHTTLLEKSFEMQEALAVRKLLERAKGYLMRAKKLTEEEAFKLIQRQSM
                     DLRKSMREIAEAVLLAGELEERAEKHRG"
     gene            complement(13218..14114)
                     /locus_tag="VFH05_07125"
     CDS             complement(13218..14114)
                     /locus_tag="VFH05_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678020.1"
                     /translation="MRFFLLGLHLFGGCVLALSGCATGPDVDETIKDSPRGSVYLERI
                     SDRSFQAAHPIKLDVATIARVLSGVYVRNDQSTLQTFLSGKPDALRAFSDEEVTFLTP
                     LIAEGLRRAAPDQQVGFRIAQIGLPGHAHNTGGGVGSSEPPLTLHPRESTTGVIHAYG
                     RSLYLTLTEYRLRTEPANTINMANRRLPDDTGLLNHTVLFVPEAAKRPDSYLDARSTD
                     KTLVIDYALLAALPASAGSPSSESSPPTAAPPASTKEKGNPAKNDGEIEALRKELDEI
                     KRQLAEQDAERARSQNKSVAPK"
     gene            14375..15760
                     /locus_tag="VFH05_07130"
     CDS             14375..15760
                     /locus_tag="VFH05_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678021.1"
                     /translation="MHEMTRFVGQIGIIALCLVAFGMGGQTAMAAEEQGAGEAAGAGR
                     LSRETETPPAVICGGCVSPTYTSQGKGSITPYGRIELDAIYSTRNTNPLDPGQFNGYA
                     TAAGKSSNTSATFNPRYSVFGLRADRTDGKHVLTGVVETDFYSQTDNAGNISPRLRLA
                     YARYSPNNNKTSITAGMDWTPVMGLHPNLIDFSIMGYNGNLWQRLPQVTVRHQFTEHF
                     DGLITAFRFERGLSAIEPQTQRRPFDGGAVGGIPPAGCANGFACSENAFNDPVQMPYF
                     GTRFGYNGTGNMQGFMVGLNAAYRYYRSAPTANVGGIPSGQNINSFLLGTELVVPITK
                     RLKFSGEIAYGQALGVEFFRYGQDRNLGTGKEIRTVVGWGELDYAHDKDTTFIAGYGF
                     DNPMNSDLVGTAAAADTQYLLNHRSYLTAVRHIWGDLFLSFEWNHLMTKWSTGESFSG
                     DNFMISTWYNF"
     gene            15793..17157
                     /gene="urtA"
                     /locus_tag="VFH05_07135"
     CDS             15793..17157
                     /gene="urtA"
                     /locus_tag="VFH05_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HET6678022.1"
                     /translation="MADKTIKSDGIQEQAGESETGSSRRDFLVHSARVTAGAGIAALL
                     GNLGHWALSYAADKEPIKIGVLHSLSGTMAISEVSLRDVVLMAVDEINAKGGILGRQV
                     KPVVVDPASNWDLFAEKAKQLLLQDKVAVVFGCWTSVSRKSVLPVFEKNNGLLFYPVQ
                     YEGEECSHNVFYTGAAVNQQAVPAVEYLMSKEGGGFKKFYLLGTDYVYPRTTNKILRA
                     MLLAKKVSEANIMEEYTPFHHQDYQTIVGKIKKFAAGGGAAVISTINGDSNVPFYKEF
                     ANQGLRAEDAPIMAFSVAEDELRGMDTTALVGHLAAWNYFQSVDTPQNKKFVADFKAY
                     CKKNNLPDGEKRVTDDPIEAAYFGVHVWKQAVEKAGKTDVDAVRKAVYGQKFLAPGGE
                     IMMDTGNHHTHKPVLIGEIMKDGQFKVVSRSKGLVKPEPWSEYTNPEKGCDWINHQGT
                     YQKK"
     gene            17392..18972
                     /gene="urtB"
                     /locus_tag="VFH05_07140"
     CDS             17392..18972
                     /gene="urtB"
                     /locus_tag="VFH05_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HET6678023.1"
                     /translation="MATARHPFVLFGDAVIIKRFGLPILLALGLSIGAAAAHGTVPPP
                     PTSPLEQALSQITSGDEAVRDAALRVVIELGDSTLVSRLDEIRANADRPVRLAIKPVM
                     DLLRNRAKLESPDPDSRRSAATDLGTSGRTVAIPWLDQAAATESNKWVRYTMQESAAL
                     LHLSGDDQAAKISAVRTLGDLASQNAVPALQEFVALGSAPEATEAQQALAKVASVSIE
                     RIETWAVWSSAMETIFRGVSLSSILLIMSVGLAIVFGLMGVINMAHGELMMVGAYATF
                     ITQELFKAFLPTAMFDYYFAAAMPMAFVVAAACGMVLELTVIRFLYGRPLETMLATWG
                     ISLVLMQAARVYFGDLTAVVAPSWLSGGVQVMVGVYLPNNRLFIIALSLVCVLAIYAI
                     LFRSGIGLRVRAVMQNRNMSACLGIPTRKVDAYTFAFGSGLAGVAGWALTLIGNVEPG
                     LGQNYIVDSFMVVVTGGVGKLAGTIVASLGIGGLNKLLEPSLGAVYGKVCILMLVILF
                     LQWRPSGLFAIKGRHADV"
     gene            18994..20133
                     /gene="urtC"
                     /locus_tag="VFH05_07145"
     CDS             18994..20133
                     /gene="urtC"
                     /locus_tag="VFH05_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HET6678024.1"
                     /translation="MSSHDHLPSGSRADVGLAFWVAGFVLLILMPLLNVMPAEDSWFH
                     LSDFSLNRFGKFLAFAILAVGLDLIWGYTGVLSLGQGVFFGLGAYCMGMHLMLTIGTE
                     SVYGNELPDFMVWNQVKSLPLFWKPFYSFPMAIVGAILVPTAFALIFGFLAFRSRIRG
                     VYFAIITQALALVAWLVFNRNETNLGGTNGLTDFKQLLGFRLSDPGTQRALYVLTVLC
                     LGGAYLLCLWITRSRAGRVLIAVRDSEPRVVFSGYTPANYKLFVFVVAAALAGLAGML
                     YVPQVGIITPAQIGVLPSLEMVIWVAVGGRGTLAGAVLGAISVNLGRSILTNYFPELW
                     PFILGGLFVVVVLLFPDGLVGIMRQIQERIHNMRVTALSAEEGLR"
     gene            20130..20897
                     /gene="urtD"
                     /locus_tag="VFH05_07150"
     CDS             20130..20897
                     /gene="urtD"
                     /locus_tag="VFH05_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HET6678025.1"
                     /translation="MSDRGSIIYLEGVVVDYDGFKALNNLNFIVNYNELRVVIGPNGA
                     GKTTLLDVICGKTRPVAGRVIFGKDQELIGKREDEIVKLGIGRKFQAPSIYANLTVWE
                     NLDLSLKRPSKGVFSTLLGTSTAEEREKIADTLMTIGLADYAGMRAGSLSHGQKQWLE
                     IGMVILQDPSLLLVDEPVAGMTDKETEQTGRLLQSLAEKHAIVVIEHDMEFVRQIARI
                     VTVLHEGTVICEGTVEKVQSDDRVREIYLGRQKVAHG"
     gene            20983..21693
                     /gene="urtE"
                     /locus_tag="VFH05_07155"
     CDS             20983..21693
                     /gene="urtE"
                     /locus_tag="VFH05_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HET6678026.1"
                     /translation="MLELKQINAYYGESHILRDVTFTVSDGEVVCLMGRNGVGKTTTL
                     KVITGLLPARSGDLVFDAENVTKVSTDRRARRGLAIVPQGREVIPHLTVRENLQLGFW
                     ARSDSPSAAVEKAAFDEVYHLFPKLTQILHRPGGVLSGGEQQQLAIGRALLSNPKLLL
                     LDEPTEGIQPSIVDQIEDVILGFKKSRRFAILLVEQGLHFAARLAEKYIVMAKGAVVA
                     AGKSDELSAEMVRQHLTV"
     gene            21894..22829
                     /locus_tag="VFH05_07160"
     CDS             21894..22829
                     /locus_tag="VFH05_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HET6678027.1"
                     /translation="MIPPLFIILAVIGSSSHAAAWDDLGHMMVSAIAYEQLTAETRQK
                     VTTLLKLNPKHATWVADVPEDEKPRIAFLRASRWADDIKLDPAYEQDGSQNGNRPSGR
                     ESARNVGYEDRLQHKYWHFIDLPFSPDRTALVGPSSPNAQTQIALFRHTLTSTASSDS
                     LKSYDLVWLIHLVADVHQPLHAVSRFDKAHMNGDDGGNGVMVCSPPCTAKERLHAFWD
                     TVLGVSMDPTTAIETAKQLPAADPQLAAITDERVWIQESFEAAQMHVYVSPIGIGVGP
                     FELTDSYKAVAQHVAGQRLALAGARLANLLNEAFR"
     gene            23066..23638
                     /locus_tag="VFH05_07165"
     CDS             23066..23638
                     /locus_tag="VFH05_07165"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HET6678028.1"
                     /translation="MKRQASRKLRLIATLAGVTMMLTGWFSGSASAASARQIDAGVDD
                     AIVKFEQEVKGGKSFMESSKGMLVFPTVLKGGAGFGGEYGEGALRIGGKTVDYYSMLQ
                     GSFGFQLGGEIKTVYLVFLDEGALKRFRDSEGWKAGVDGSVSLITLGAGGTIDTNNLK
                     DPIVAFVLGQKGLMYNLSLEGTKFTKQHKK"
     gene            23658..25040
                     /locus_tag="VFH05_07170"
     CDS             23658..25040
                     /locus_tag="VFH05_07170"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6678029.1"
                     /translation="MGLVCSRMVRAVLHFLARSSSHRAGSNCVVRPLDSLHAICYSTL
                     FVTCILAPGALLADAWAEDTPGPVAQRRLELEHIALRLKEAEKAVEKRLLSESLRLHF
                     YGYLEGSYTQNFNNPSNRINQLRVFDVNSNQFRPNLAQFVLEREAKAEATGWDRVGFR
                     VKFNAGRDSDFIGGINLSTWADFQEFYGQYRAPVGRGLTLQAGQFNSVVGYEVVESPH
                     NTHYSRSWLFGLGQPFTTRGLRATYEFNEQVSLSVGAIAYINSARADTNFDPLVEYAL
                     VVTPTEKVRVTVYGLAGPRDGPSGTPGGTVFLAGGYLSVQVTDHTSAVIESYYANQSN
                     SSTIKTSRNARWDGAAAYVIHDFTDRWGLRLRGEIFEDAGGFVTCQGTTDYQPRANVC
                     FGATSGASAPAVAQTLWEFTGTVQYKPFASFITRLEYRYDKSNQNVFQVGGRATSYQP
                     TLSLDVIYLF"
     gene            complement(25028..26080)
                     /locus_tag="VFH05_07175"
     CDS             complement(25028..26080)
                     /locus_tag="VFH05_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HET6678030.1"
                     /translation="MARVALRDRFREHPVWGFGDWVLRGIAQVVFQNNPITGALILAG
                     IFYNSWIYGLACLFGTIISTATAVLFKADKGLIKDGLFGFNGALVAVALVAYTSPNFT
                     TGNMPNLHLWLYIVLCAVFTSVILPAFGALLGPHKVPALTMPFVLATWFFLGALLQFT
                     TIDVSNAIKPTSPADFTGPRPEYSWLTWFHGMTMGISEIFFQDNWMTGIIILLAIAVN
                     TRLGALMALMGTTLAAGSAIFYGAHEEAIRDGLFGYNAALTAMALGGVFYVLNLPCLV
                     YTIIGILVTTRVWASLGIFLEPTGMPVLTSAFVFVTWLMLLAKPGFSRSLIPVAPGDA
                     TTPEENLARHRASQKR"
     gene            26198..26500
                     /gene="ureA"
                     /locus_tag="VFH05_07180"
     CDS             26198..26500
                     /gene="ureA"
                     /locus_tag="VFH05_07180"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HET6678031.1"
                     /translation="MHLTPREQEKLLIYTAGQLAADRMKRGLKLNHPEAVAYLTAAVL
                     EGIRDGKTVSELMTYGTTLLSYDDVMPGVPEMIHEIMVEGTFPDGTKLVTVHNPIR"
     gene            26528..27001
                     /locus_tag="VFH05_07185"
     CDS             26528..27001
                     /locus_tag="VFH05_07185"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HET6678032.1"
                     /translation="MPKTKHRPAGNAATKSASSLAGAIKAGLAKPVIPGEILFAPGDI
                     EAFKGRTTKDVTVKNTADRPIQVGSHCHFFEVNRGLLFDREQAFGYRLCIPAGTAARF
                     EPGEEKRVTLVALGGARTVQGINGLTDGNLDDAQAKARALARAAEHGFIKKGGGR"
     gene            27004..>28652
                     /gene="ureC"
                     /locus_tag="VFH05_07190"
     CDS             27004..>28652
                     /gene="ureC"
                     /locus_tag="VFH05_07190"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET6678033.1"
                     /translation="MPRKQYADLFGPTTGDRVRLADTDLLIEIEKDYTSYGDEAVFGG
                     GKVLRDGMGQSARMTNADGALDTVITNALILDYWGIVKADIGIKNGRIVGVGKAGNPD
                     TMPNVSPGMACGAGTEAISAEGCIVTAGALETHVHFICPQQCWEALSAGITTMIGGGT
                     GPATGTNAVTSTPGPWNIHRMLEAAEGIPINLGFTGKGNCSLPGPLNEQIEAGAIGLK
                     VHEDWGSTPAAIDSALSVAERYDVQVALHTDTLNEAGFVEDTIKAFKGRTIHSYHTEG
                     AGGGHAPDVIKLCGEPNVLPSSTNPTMPFTVNTLDEHLDMLIVCHHLNKRVPEDVAFA
                     ESRIRRETIAAEDILHDMGAISMMSSDTQAMGRIGELIIRTWQTAHKMKLQRGHLTER
                     GLQSGKGQNDNFRAKRYIAKYTINPALAHGVAHEVGSLEVGKVADLVIWKPAFFGIKP
                     EMVLKSGFIAQAQMGDPNASIPTPEPIISRPMFAAFGRAVASTCLTFVSAAALERDVP
                     HQLGLQRRIVAVKGCRTVHKRDLKLNDALPQIEVDPETYVVT"
BASE COUNT         6362 a         7832 c         8570 g         5888 t
ORIGIN      
        1 cggggtcaag ctcgatgcta tggcctcagg cagcggttct aaaaacgaat agaaagaggg
       61 caccgtgcgt caccgaaaaa aaggcagaca gctggggcgc cagacaaaac accgctgggc
      121 cttattcaga agcctcgtca cctcgctact cgaacatgag cggattgaga cgactgaagc
      181 caaggccaag gagattcgtg gttttacaga ccgcatgatc acgcttggaa aggacggctc
      241 cctgccggcc cgccggcgtg cgttgagctt cctgcgcagc aaggacgtgg tctccaagct
      301 ttttagcgat gtggccggac ggttcaagga tcgtcccggc ggatacacca gaatggtaaa
      361 gaccaggcgg cggatcggcg atgcggctga actagtcgcg attgaactcg tggcacgcgc
      421 cgaagtctcg acctcaaaaa agcccgggtc gactccagcc aagacagaca aaacggagta
      481 ggtctcgcct cgagacctac tcgcgacgcg cggcttgacc gccgtcgtgt gatcaatcag
      541 aacctcgcct cagcgctgca tgttttttcg ttgctaacgg cgcgcagagt ttacttgcca
      601 tcagtcggat gctattatcg tgccaggctt ttctccttga ggcaccggac ggaaacattg
      661 tgtggcagcg ttgggcacaa cggattttac tggggcttag cctcgctctg gtgtgcttcc
      721 tggtctacct cctcatgacc aactccaccg cgtctcctcc tcccacggcc acggcgccgg
      781 gcaccattaa tcaggccgat gccatcattt ccaagttcac ttttacgcag accaaagggg
      841 acaaagtgca gtggcaggtc gacgcgcaac aggcgcgcct ctacgaacag gacaagaggg
      901 cgattctgga caacgtcgcc gtgacgctct tcggtgtaca ggggaaggaa atgacggtgc
      961 atggggatga aggaaccttg aataccgaca ccaaaaactt tcttctcacg aaccggtctg
     1021 agccattggt gatctacaca cagagcggct atacgattta taccaaccat ctggcttgga
     1081 cggatcaaac acgggagatc cgcacacagg accccgtcca tatcatcggg cacggactgg
     1141 atgtgaaagg acgaggcttg cttggtcacc tcgacacgga agaattcgaa gtacttgagg
     1201 acgttcatgt ggatgtggct cctgcttctt agctggtgcg cgatgagtat gccgacggtc
     1261 ggtcaggctg ccatgggggc cggctcgaac ggggcatcag acgaagcgct aggaaccacc
     1321 gtcatctccc aaaggatgac atttaagaac caacaaagcc aggccgtgtt cgatggcgcc
     1381 gtcgtgctca cgcgcggcac ccttgtcgtg tattcggacc gcatggtggt gtcctttcga
     1441 aaccaagagg cttcggattc caaaaaagcg agcgaaccgc cgaagggggg acccgcacca
     1501 gctaaaggcc cggacacggt gtcgaatcgg gcagtcagcc aaattgaagc cacgggccgc
     1561 gtcaaaatcg agcgggacgc aggcaacgcc acctgccaaa aggctgtcta ttaccgcgac
     1621 gaagacaaga tcgtcctgac cggtgatccc gtcgcatggg acaagggcac ccgagtcagc
     1681 ggcaagcgca tcaccatgtt cctggctgaa gatcgaacgg tcgtggaagg cggctcacac
     1741 gtacgaatcg agccggaggg tggtaccaag tgacggaagc ggtgcgtctg gcgccggaaa
     1801 ccgatggtgt cgcccgttcc agcgagccgc gtgcggtgtt gcgcgccgat ggcctcgtga
     1861 aaagttttcg ggcgagaaaa gtcgtcaaag gtgtttcggt ggaggtctcg gccggagaag
     1921 tcgtaggttt gttgggccca aacggtgccg ggaaaacaac catcttcgat atgatcgtcg
     1981 gcttgaccca gccggacgaa ggctcaatcc gcctgaacgc tgaagagatc accgccctac
     2041 cgatgtacaa gcgtgcaaga aacggcatcg gctacctgcc tcaggaatcg tcggtatttc
     2101 gccgactgtc tgtgcaagac aatattctgg caattctgga aatgctggat tactctcgag
     2161 ctgagcgggc ggaacgcgtc gatgtcttgc tcggtgagct cgatctcgga cacatcagga
     2221 cgagcatggc ctacgcgctg tccggtggag aacgccgccg gttggaaatt acgcgtgcct
     2281 tggcgaccaa ccccacattc atgctacttg atgagccgtt cgccggcatc gatccgattg
     2341 ccgtcgcgga cattcaacag atcatcacga ggttgaaggg caaaggcatt ggcattctga
     2401 tcacggatca taatgtgcag gagacgttat cgattaccga ccgtgcatac atcatcaacg
     2461 agggcctcgt tctagaatcc ggtcctcctg atgtgatcgt gaagagcgag acggcgcggg
     2521 cggtctattt gggagaacgg ttcactctat aacgggcgtg cttatcaatg aaacttcgtc
     2581 ttgatctccg cctcagccag aaattaatta tgacgccgca gttgcagcag gccatcaagc
     2641 tgttgcagct gtcacgcctt gagttgcagc aaagtctggc tcagcatttg atggaaaacc
     2701 cgttgctgga cgagctgccc gaggaagtgg aggagaacga atcgacgacc gccgaggaaa
     2761 aagcggaaga agctgcggcc tctgcctcgc cggctaacga gacttccgaa acggatgaat
     2821 cctcgacccc agaggaacgg gacactcccg acgaagtgtc ggcggccggg tgggaggagt
     2881 acttcggcag tgaccgccgg acaggcggca gcgaaggtca atcgtcttca tcggatgatt
     2941 ttccctccta tgagcagacc gtcgccaaag cggcgtcgct ggaagaccac ttgctctggc
     3001 agctgtcctt atccggcttg tcggagcgca ataaggcgat cggtcgtttg ctgatcggga
     3061 atttggacga cgacgggtat ctgcgcacgc ccctgtccga aatcgtcacc ggaacggact
     3121 tcacggaggc agaagccgag tccgttctcc gcgacatcca gacgtttgat ccgacggggg
     3181 tgggcgcgag ggaccttccc gaatgtctga tgctgcagct cggccatttg ggcaagagtc
     3241 cgatggggtc cctcggcgcc cgtccgggcg cgctcaaagg atccatcgtg gagtcggtcg
     3301 tcctccatca cctgaaggac ctcgaaaaga agcaatatgc caaagtcgcg aaagcgctcg
     3361 acgtcaccgt cgaggaaatc ttccaggcca caaaggttat cgaagcgctc gaacccaagc
     3421 caggtcgccc cttttccaat actcaaaact acgtcatcgt gccagacgtg ttcgtcgtca
     3481 aaaatgaagg ggagtgggtt gtcctattga acgacgacgg attaccccgg atgcgcatca
     3541 gcccctacta caaacagatg atggcctccg gtgagggcgg atccacagaa accaaggcct
     3601 atctcgatga aaagttgcgg gccgcccaat gggtgatccg gagtatcgag caacgcaaca
     3661 agactatcgt caaggtcgta tccagcatcg tgaagtttca ggagcaattt tttgaaaagg
     3721 gcattcagta cctcaagccg cttgtgctca aacaggtcgc cgaggacatt ggaatgcacg
     3781 aatccacgat cagccgtgtg acggccaata agtacatgta ctgcccgcaa ggcatgctgg
     3841 aactcaagtt ttttttcaat gccggtctgc agcgcgcaga tcagcccagc gacatgcttt
     3901 catcagtgac agttcgggag atgatcaaga aaatggtggc ggaggaggat gccaaccgtc
     3961 cgctcaagga cgaggaaata gccgcgcgct tgcgtgagcg tcaagtcttg atcgccagac
     4021 ggacggtcgc aaagtaccgg gcggaagaga atatcccgcc agccacccaa cgcaaacgct
     4081 ttttttaagc tagtgccaga cctgcggact tctctccatt cgaaggagtg cagtgcatga
     4141 aaattaccgg acgacacatg gatgtcacct ctgccctgaa acggtatatt aagtcaagat
     4201 tcgagcgcct cgaacgatac gatgtctcac cggaccgctt ggagatcgtc ctgagcgtga
     4261 cgaaacacca acataccgcg gaagtggtgt gctcgatcag aggaaagcgt ttccaggcga
     4321 agacgtcaac gacagagatg tatgcgacgg tcgatcaact ggtcgaccgc ctcgacaaac
     4381 agatccggaa gcacaaggag cgccggattg cgcacaaggg aagaacgaag acgcagctcc
     4441 ccgctacggt cggagcgtcc cctgatttga acgacctgaa aatcgtgcgt cccaagatcg
     4501 ctgtcctgtc gcgcaaagag gcgcgtgatc gcttggacgc tgatccgggt gccatgctgt
     4561 tgtttacgtg cgtggattct ggaaagttgc aaattcttcg gcgatctgag accggtcacg
     4621 tcgtcctcat tgatccttga agggtcgaga tgccaggact caagctcgtg gttatcaccg
     4681 gcctctctgg agcggggaag tcgcacgcgc tgaaatgctt cgaagacgtc gggtatttct
     4741 gtgttgataa ccttcctcca gccttgcttc ccaccttcgt tgaactctgt caccagcagg
     4801 gcggcgagat caaaaatgtc gcgcttggca tcgacatccg ggagcgggtg ttctttgccg
     4861 acttgaccgg catcctgcag cggatcaagg acctcgggca ttccgtcgag ttattgtttc
     4921 tggaagcccg tgatcccgtg ctcgtccgtc gcttctcgga gtcccgcaga ccgcatccgt
     4981 tgttgcccga gttgcctgtc ctggaaggcg tgcggttcga aaaagagcgg ttagcggaaa
     5041 tccgggcgca cgccgatcgc atcatcgaca cctcgaatct cagcgtccat gatttgcgcg
     5101 atttactgag ccggcatttc agccaggaac tatccgcccg acgcctgacc atctcgttga
     5161 tcacattcgg ttacaagttt ggcgtgccgt acgacatcga tctgttgttc gacgttcggt
     5221 tcttgcgcaa cccctatttc gtccctgatc tgaagccgct cacgggcgag gatccccggg
     5281 ttcggagtta tgtgctcgat gatccggatg ccaagggctt cctggatcgg ctggagcagt
     5341 tctttaccta tctgatcccc ctctttgaac gtgagcgaag gagctacttg aacgtcggga
     5401 taggctgcac gggcggtcgc caccgttcag tcgtcatcgc caagcggctg cacgaaagtt
     5461 tttcttcggc cgggcatcac gtcactctct cccaccgcga tctcaccaag ccctaggtcg
     5521 ctctgctctg atctttaggc ctccgagcat cctcggcgac gcccctttgc ccgcttttct
     5581 tgacagtgag gacacatgga ctatacttag ggggcggcgt aagaatcacc aatattcaag
     5641 ccactttggt gcgtgttgcc tctttgccat cgtctgaaaa gtgacgcctc tatcggatgc
     5701 gtgttggaaa aatgcaaaaa gatttgcgct gagaaagaag tcatgtacag tttgaggcac
     5761 atatgctgaa ttcactgaaa agcctcgtcg ataaggacat tttttcgctg ctgactccca
     5821 agcggcagtt ggtcggcttg gacatcggat ccagcggcat caagctcgtt cagctcaagg
     5881 agaaccgtgg gcgctatgtg ttgcagaagt tcggctttaa gcccttggag cctgaggtca
     5941 tcgttgacgg cacggtgatg gatgaaggcc aggtcgtctc ggccatcaag gcactgtttg
     6001 atgaacttaa tgtcaaggtc aagcaggtgg cggtttcgat ttctggccat gcggtcatca
     6061 tcaagaagat cagtttgcct ccgatgccgg acgacgagct ggaaggccag gtcaaactcg
     6121 ccgcagagca atacattcct tttgacatta atgaagtcaa tatcgatttt catgtgcttc
     6181 cgccgtcgga aggtgacgga tctggtgaag cggaaatgtc ggtcattctt gtggccgcca
     6241 agaaagacaa gatcaacgag ttgacggagc tcgtcaaggg tgccggactc ctgccgttgg
     6301 tgatggatgt ggacgcattt gcgatcgaaa acatgcatgc gatcaactat ccgatgtccc
     6361 aggaagagac gaccgctctc gtgaatgtcg gtgccagcgt catgaatatc aacatcgtct
     6421 cgaaaggaac gtcgctcttc acgcgcgaca ttccgatcgg cggtaaccgc tatacggaat
     6481 ccatccaacg ggagctgggg ctctcttatg aagaagcgga acaactgaag aaaggtggaa
     6541 aagcctccgg gaataaccag gcggccgctg cccaaagcgt gatcgagagc gtgaatgccg
     6601 aagtggcctc tgaaatcgcg cgcacgatcg actattacaa gtcgacagcg gctgaaggtg
     6661 aagtacaaca agtgctcctc tgcggaggag gagcgcaagt ctctgggctg gtaaaacagt
     6721 tgcgagatcg gatgcacgcc gttgtcgagg tggccaatcc gttcgcggaa gttgacacgt
     6781 ccggcagcga cttcgaccag acgacgcttg ccgagatggc ttcgatggcc tccgttggag
     6841 tgggattggc cctcagatcg gtaggcgatc gatgattcgc atcaatctac tgtcggcagg
     6901 accaaaggga cgtcaggcaa agccgcaata cgacgtgcgg gctcaagtgc ttctgggcgt
     6961 cgggttgctg gtcatcacct tgagtgcatg ctggtggtat tcggcatccc tcgatgatga
     7021 gattgcgacg cgccagacgg aaaaatccga aaaagagaag caggtggcgc agctaaagga
     7081 acaggtcaaa caggtgtcgg acttcgagct gcgaaaaaag caacttgaag acaagaaccg
     7141 cattatcgat caactcgaga agtcgcgcgt gggtcctgtc aaagtcatgg atcatgtcag
     7201 tcacagcttg gaaccactga aactctggct cgtgaaggtc ggcgtgacgg gacaaagcat
     7261 cgaattggaa gggcgggccc tgaccaacga cgacgtggtc gagttcgtca ataatctacg
     7321 ccgtaccgac tatttttccg acatcaatct tcaggaaagc cgggctgccg ttgagagcca
     7381 aatcagcctg ttccagttca agttgggctt ccgcttgaaa ggatagcgag catgaacttg
     7441 ccggcgatga atctcgaggc catacgaacc atcccgttga tgcaaaaggt cgcgtttatg
     7501 gttctcgtgc tggccggaat cgtcgtgatg ttctattact acgtcttcga gccgaagtcg
     7561 atggagatcg ctgctctgga aggcgaagtg ggcaagctgg ataccgagat ccaaacattg
     7621 accatcaaag tcaagcatct ggatgaactc atcgccgcca gcaaacagct ggagatcgaa
     7681 ttggcaaaga aaaaagagcg cctcccgccg gaagaagaag ccgtcatgtt gttgaagcaa
     7741 gtgtcggatc tcggcatcaa gctgggtctg gacattaagc tctggaagcc cggcgccaag
     7801 agcgaggatc cttccaagtt attcgtgcgg ctacccgtaa atgtcgaggt gacggggggc
     7861 taccacacgg cggcgctgtt tttcgaccgg attaacgggc taccgagaat tgtcacggtg
     7921 tccaatttga agatgggcat gccaaaaacg gaaaagggcc gcgtggttac acagacggtg
     7981 ttcgatctgg tggcctatgc ggcgcctcag gatgtgaaag tggctgcggc acctgcgcct
     8041 gcgccgaagt aaggaatgtt gaaggatgcg tgtggatcac atggttaaaa tcggcggtct
     8101 cgggacattg ttcggctgta tgctcttgct cgcgatgatg ggacggtcat tggctgagcc
     8161 gggcctcgca tcccatctcg gacaagttgc tccgcttaga caagattcct tgaaagtgcc
     8221 ctcgcctaat gatctgacaa aggagccgcc gcagtcagcc ttgacgctgt cacctgatct
     8281 cgctgccgct ccgacggatg cattgccggg cgcgccgccc tttccgatcg ggggctacga
     8341 tccggccgga cgtcgggatc cgttctcgcc cgtcctcagc cagcttgctc caggtcagac
     8401 cgatcccaca ttgccgccct tacagcgcat tgggctgacg gatttgaatc tcattgcgat
     8461 tatctggggc gcatacggat acaccgcgat ggtgcagaca ccagatggaa acgggtacac
     8521 ggttcgaaag ggcaccaggg tcggacccaa tagcggcgtg gtcagcgcgg ttaccgaaaa
     8581 agggattatc gtgcaagagc ggtttaccga cgtgtatggc agaaaacagg agcgcgagta
     8641 cgtcaagctt cttcatccca aagagggcct agaatgacac acatacagag acacgcgtcg
     8701 acgacggttc gaatcctatc tgccttggcc gcggtcagtt tgcctatggc catcccctcg
     8761 acgggggcct ccgcggatgt gaacgccggc gtgtccatga accgccccgg catcgaccag
     8821 ggagcctccg acagacggct ggccagcgcc gtgacaggga ttgatgtcaa aaaagaggcc
     8881 gatgccgtca gcgtgactat ctccggtgat ggttcactac tgcacgaagc gctccgagta
     8941 gacgagcgtc gactggtcgt ggatatccca cgagtctcct ccgagatccg cagacccact
     9001 ctgaatgtcg ggcaccgcct ggtgaaacaa attcgtattg gagatcacag cgacaaggtc
     9061 cgcattgtgt ttgatctagg tcagcaagca gagtacgccg tggagccgca gggggcaagc
     9121 ctgctcgtcc gggtgcacga gtccataggc ggcgggagca tgcccgaaca ggacagcacg
     9181 aatgctcaca cgagtgctgc tccgtccgcc gcggcagaca ttcctctgat cgagtccgct
     9241 cagggcggac gacacagggt acagccggtg gtgcatcgga caacgccggg atttcgagtc
     9301 cgtccgattc aaatgatggc cgagccggat gcggtggaaa aacatacgcc gaaagacgac
     9361 ctggtcgttg gcgagacccg ttatgtcggc cgtcgtattt cgttagactt ccaacaggcc
     9421 gatatcagta atgttctgag gttaatcgcg gaggtcagcg gcttcaacat cgtgatcggt
     9481 gacggcgtca aaagcaaggt cacgatgaag ctcgtcagcg ttccctggga ccaagcactg
     9541 gacatgattc tcaagatgaa cggccttgga aaaattaaac agggaaacat cctctggatc
     9601 gatacgctgt cgaacattgc caagcaagag gacgaggagg cgagagcgaa ggatgccaag
     9661 gtcaaagcgg aagacctcgt tgatcgtgtc ttctatatcc gcaatctcca ggcaaccgag
     9721 ttgatgacct cgttgcggca gaatttgagc cccaggggcc tcatgcagat cagcaccggc
     9781 accaacgcgt tggtggttcg cgatactgaa acgaagatgg ccgtgctcaa acaactgatt
     9841 gacggattgg atctcgaggt gccgcaagtc caaatcgagg cacgcatcgt tcaggccgat
     9901 acggtctacg cgcgcggcct tggtgtccaa tggggcattg cgaatgccga tttcggaacg
     9961 aatacgttct cggcagtggg gaacctcacg ggtgcattcg ccccggctgc aggaacgggg
    10021 gcgtcggtga ttccccgtga tttcctggtg aacctgccgg cgcaggtggc tggtcttccg
    10081 tcggtgccgg gcataggcta ttcgttcggc aaactggcgc ccggttttgc cctggacatg
    10141 aggctctctg ccggagagtt gctcgggttg gccaaggtga tcgctgcgcc gaaagtgacc
    10201 acgctagaca agcgggaagc gaaaatttcg cagggcgaat cgattccgtt ccaaacgact
    10261 tcacttcaag gcacgcagac caccttcgtc gatgccaact tggaactgaa cgtcactcct
    10321 cagattacgt cgcgtgatcc aaaagaagtc ggtaagcaga tcatgatgaa agtgcgtgca
    10381 actcgaaatg cggtgggcgc gcgcagcaat ccggccggtc cgagcatcga tcgtcgagag
    10441 gccaacaccc aagtcatcgt gcgggatggc gaaacgatgg tcatcggcgg cgtctttatc
    10501 gatacacaga acaacaacgt tcagggggta ccgtatcttt cccggattcc ggtgctcggg
    10561 tggttgttca agaacaagtc ggaaagcgtg tcgaaacaag aactgttgat cttcctgact
    10621 ccttccatcg tcaaggcctg acggcccctg cgatccctga ccgaggggat tgcagggtcg
    10681 tgttctccct ctctgtggct gcctgtcctc ctcccgtttg actgctcttc tctcagtctt
    10741 gaaagctatt atccttcaga ggcttgtgct actattcccc ggtttacttg tacacgacac
    10801 atcctaccgc cacgatgaca agcatgatcg gtcaacatgt ccctgttcgt ctcggcgaac
    10861 ggagctatga catcgtcgtc gagccgggct tgagtcggtc tctcggcgcc cttctcaagc
    10921 ctcttatgga caccggcaag gttggcattg tcaccgatcg ccatgtcgcg cgtcactatc
    10981 tcaagccgac gattgagtcg ctgaagcagg ccggatacga accagtcacg atcgtcctcc
    11041 cccctggtga gcggtcgaag acactgacga ccatcgctcg catcttggac gtactggcca
    11101 agcagcgatt cgaacgccgg tccctgctgc tggcgctggg cggcggcgtg attggcgatc
    11161 tcacgggatt tgccgcggcc atctaccagc gcggcattgc ctatgtgcaa gtgccgacca
    11221 ccctggtggc gcaggtggat tcaagcgtcg gaggaaaaac aggcgtcgat catcgcctgg
    11281 gaaagaattt gatcggggcc tttcatcagc cgcgtgcagt cttcatcgac ccgttcacgc
    11341 tgcacacgtt gccgcgccgg gaatggattg cgggactggc cgaagtgatc aaatacggtg
    11401 tcatcgccga tgaagtgttt ttctcacgtc ttgaagcgtc catgtccgat cttctcaagc
    11461 ggaatgcgca tgccgtcatg caggctgtga cacgctcctg ccaaatcaag gctgaagtcg
    11521 tcgcggcgga tgagcgggag tctgaccggc ggcgcatctt gaactacgga catacaatag
    11581 gccacgcgct ggaagtgctg tgcaattatc gaaatctcat ccacggcgaa gccgtggcta
    11641 tcggcatggt cgccgaagcc gacctgagcg tccatctcgg cctctgtaaa caggatgtct
    11701 ccgctcgtat caggggcctg gtccaggcgg cgggtcttcc cgatcgcatg cccgacacgt
    11761 catttgctgc tctatggcgc gccatgcagc acgacaaaaa agttgtgggc ggcaaagtga
    11821 tgggcgtctg gccgattcgt ctcggcgaag tcgtgatgcg tccgctggag tcggaggcct
    11881 gcgccgactg gcatgccaca ctgtccggga gcaattctgc tcgacagaaa cgcagaatac
    11941 gaagggctta gccggtggcc aaacgtcccc cctcactgac gcaactcgag caagccctcc
    12001 gggaaaaaac ccgggaggtg gacgtgttgc accggatcac cgattccatc agcaacaccc
    12061 tcgatctcga agcggtattg aagcacatcg tcgagattgt cgtcgaagtc acgaaggccg
    12121 acgcctgttt gctctacctc ctttcggacg gcgaggacga actgatcttg cgggcctcga
    12181 aaaatccaca tcctaaactc atcgggcgga tcaccatcgg tatgggtgag ggcatcacgg
    12241 gctgggtggc gcgcgagcgc acgcgagtcg tgatcccgag caatgcgagt gacgatccgc
    12301 ggttcaaatt ttttcacaat ctgcccgaag accggcacca ggccttcgtg tccgtcccga
    12361 tcatggccaa gaaggaagtt gtcggcgtga tcaatgtgca acacaagcgt cccaagcggt
    12421 atcggccgga cgagttggcc ttgctgtcga ccatcgccaa tcaggtggga ggggcgattg
    12481 aaaacgcgcg attgtacgaa cagatgaggc gcaaggcctt gcaagtcgaa acattgtccc
    12541 aggtgtcgga aaccgtggca tcgaaccgcc tgatcgagga tgtgatgcag ttgcttgtga
    12601 cgatgacggc gcaaatgatg aactcgaaaa tttgttcgat catgttgctg gacgagacga
    12661 ccggagatct gcggatcgag gcgacgcaaa gtctcagcga acattaccgc cggaagccga
    12721 atctcaaagt ggggcaaagt gtgagcggcc gggccgtgca ggaacgccgc ccgatcatgg
    12781 tcagcgacgt cacgaaagaa caggactaca tgtatccgga tatggcaaaa aaagaagggc
    12841 tctgctcgct cttgtcggtg ccgatgctgg tacgcgaaaa agcggtcggc gtgatcaaca
    12901 gctacacgtc cgtgccgcat acttttacgg ccgaggaggt caagctctta caggcgatcg
    12961 ccaaccaagc ggccattgcc attgagcaca cgaccctgtt ggaaaagtcg ttcgagatgc
    13021 aggaggcgct ggccgtccgg aagctgcttg aacgggccaa aggctatctg atgcgtgcca
    13081 agaagctgac ggaagaggaa gcgttcaagt tgattcagcg gcaaagcatg gacttgcgaa
    13141 aatccatgcg ggaaatcgcc gaggcggtgc tgttggccgg ggaactcgaa gaacgggctg
    13201 agaaacaccg agggtagtca cttcggtgcg acggatttgt tctgcgagcg ggcgcgctcg
    13261 gcgtcctgct ccgccaattg ccgcttgatc tcgtccaatt ccttccggag agcttcgatc
    13321 tcgccgtcgt tcttcgccgg gtttcccttt tccttcgtcg aggcgggtgg tgcggccgtc
    13381 ggcggcgacg actccgaaga cggcgatccg gctgaggccg gtaaagcggc taagagcgcg
    13441 taatcgatga caagcgtctt gtccgtggaa cgggcatcca gatagctgtc gggccgtttg
    13501 gcggcttccg gcacgaacaa gacggtgtgg ttgagcagac ccgtgtcgtc gggaagtcga
    13561 cggttggcca tgttgatggt gttggccggt tcagtgcgaa gccggtattc cgtaagcgtc
    13621 aaatacaggg atcgtccgta cgcgtggatg acaccggttg tcgattcacg gggatgaagg
    13681 gtcaggggcg gttccgatga gccaacgcca cctcccgtat tatgtgcatg acctggaagg
    13741 ccgatctgcg cgattcggaa accgacctgt tgatcagggg ccgcgcgcct gaggccttct
    13801 gcgatgagtg gcgtgaggaa tgtcacctcc tcatcggaaa acgcccggag cgcatcgggc
    13861 tttcctgaca aaaaggtttg cagggtgctt tgatcgtttc gaacgtacac gccactcaag
    13921 acccgggcga tcgtcgcgac atcgagtttt atcggatggg cagcttgaaa agatcggtcc
    13981 gaaatccgtt cgagatacac cgatccgcgt ggagaatcct tgatcgtttc gtcgacgtcg
    14041 ggtcccgtcg cacaaccgga aagggctaga acacagccgc caaacagatg taagccaagc
    14101 agaaagaacc gcattctcct ccgtcaatta ctggagcgcg cctcagtcta gcacaggttt
    14161 caagaagctt gacagcgcga gcgggaacac ttataagtac cgcccattaa atcgagctga
    14221 agtacggaca aggaagtccg attttcagtc ggcatatgtc aaaggacaat ggcgtccttt
    14281 tggtcctgga tgcggaccgg aagggcgctt ttttttgaac gctgccctcc ggcgtgtatc
    14341 tgttgttgag cagttaacga agagggagac agccatgcac gagatgacaa gattcgtggg
    14401 tcagatcgga atcatcgcgc tctgccttgt ggcgttcggt atgggtggac agacggcgat
    14461 ggctgcggaa gaacagggag ccggtgaagc ggccggagcc ggtcgcctgt cacgcgagac
    14521 ggaaactccg cctgcggtga tttgcggagg ctgtgtgagc cctacctata cctcgcaagg
    14581 caaaggcagt attaccccct acggccgcat cgagttggat gcaatttaca gcaccagaaa
    14641 caccaatcca ctcgatcctg gacaattcaa cgggtatgcc acggcggcag gcaagagcag
    14701 caatacgtcg gcaacgttca accctcgtta cagcgtgttc ggccttcgtg cggaccggac
    14761 cgacgggaag catgtgctga caggtgtggt ggagacagat ttctattcac aaaccgataa
    14821 tgcgggaaat atttcgcccc gcctccgtct cgcctatgcc aggtactcgc cgaacaacaa
    14881 caagacgagc atcacggccg gtatggattg gacgccggtc atgggtcttc accccaacct
    14941 cattgacttt tcgatcatgg ggtataacgg caacctgtgg cagcgtctgc ctcaagtgac
    15001 ggtgcgtcac caattcaccg agcacttcga cggtttgatc acggccttcc ggttcgagcg
    15061 tggtttgtca gcgatcgagc cacaaacgca gcggcgaccg ttcgatggcg gtgctgtcgg
    15121 cggtatccct cctgctggct gtgccaacgg atttgcctgt tccgaaaatg cctttaacga
    15181 tccggtgcag atgccctact tcggcacccg cttcggctac aacggcaccg gcaatatgca
    15241 agggttcatg gtgggattga atgcggccta ccgttattat cgttctgccc cgacggcgaa
    15301 cgttggcggc atcccctcag gtcagaacat caattccttc cttctcggca ctgagctcgt
    15361 ggtcccaatc accaaacggt tgaaattctc cggcgagatc gcctatggcc aagcgttggg
    15421 agtggaattt ttccgctatg gccaggatcg caacctcggc accggcaagg aaatcagaac
    15481 ggtggtcggg tggggcgaac tggactatgc ccatgacaag gacacgacct ttattgcggg
    15541 atacggattt gacaatccga tgaactcgga tctcgtcggc acggccgcgg ccgccgacac
    15601 ccaatatttg ctcaatcacc gctcctactt gacggccgtc cgccacatct ggggggatct
    15661 gttcctctcg tttgagtgga accacctcat gacgaaatgg tcgaccggcg agagcttctc
    15721 gggcgacaac ttcatgatct caacctggta caacttctag cagcggtgtt tcgtaccgaa
    15781 aggagttcgg caatggcaga taagacaatc aagtcagatg ggatacagga acaagcaggc
    15841 gagagtgaaa ccggttcgtc acggcgcgac tttctcgtgc acagtgccag agtgacggca
    15901 ggagcgggca tcgccgcgct tctgggcaat ctcggtcact gggcgctgtc ctatgcggcg
    15961 gacaaggagc cgatcaagat cggcgtcctg cattccttga gcggcaccat ggcgatcagc
    16021 gaagtgtcgc tgcgcgatgt ggttctcatg gcggtcgatg aaatcaatgc caaaggcgga
    16081 atattgggcc ggcaggtgaa gccggtggtt gttgatcccg cctccaactg ggatctgttc
    16141 gccgagaagg cgaagcagct cttgctgcaa gacaaggtcg cggtcgtctt cggctgttgg
    16201 acgtcggtca gccggaagtc tgtgctgccg gtgttcgaga agaacaacgg cttgctcttc
    16261 tatccggtgc agtacgaagg tgaagaatgc tctcacaacg tattctatac cggtgcagcg
    16321 gtcaatcagc aggcggtgcc ggccgtcgag tacttaatga gcaaggaagg cggagggttc
    16381 aaaaagtttt atcttctcgg cactgactac gtgtacccgc ggacgacgaa caagatcctg
    16441 cgcgccatgc ttctggccaa aaaagtgtcg gaagccaaca tcatggaaga atacaccccc
    16501 ttccatcatc aggattatca aacgatcgtc gggaaaatta agaaattcgc ggccggcggc
    16561 ggagcagcag tcatcagcac catcaacggg gacagcaatg tgccgttcta caaggaattt
    16621 gccaatcagg gcctgcgcgc ggaagatgcg ccgatcatgg cgttcagtgt ggccgaagac
    16681 gagctgcgtg ggatggatac caccgccctg gttgggcatc tggctgcctg gaactacttc
    16741 cagagcgtgg atacgccgca gaacaagaag ttcgtggccg atttcaaggc ctattgcaag
    16801 aaaaacaacc tgccggatgg agaaaagcgt gtgacggacg atccgataga ggcggcgtac
    16861 tttggtgtcc atgtctggaa acaggcagtc gaaaaggccg gtaagacgga tgtggacgcc
    16921 gtgcgcaagg ccgtctatgg gcaaaagttc cttgcaccgg gcggggagat catgatggat
    16981 accggcaatc atcatacgca caagccggta ttaatcggcg agattatgaa ggacggccag
    17041 ttcaaggtgg tctcccgctc aaagggattg gtcaagccgg agccttggag cgagtacacg
    17101 aatcccgaga agggttgcga ttggattaac caccagggca cataccagaa aaagtaggca
    17161 ctcgagagaa aaacccacgc tgccgcagcc cggtgcaggc cgcgtctcgg cgcggcgcgg
    17221 ggtgggcggg tgaatcaagt gcaggccgcg tctgggcgcg gcagggtggg cgggtgaacc
    17281 aggtgcaagc cgcgtctcgg cgcggcgggg ggggtggctg aaccagcacc aggtccatgg
    17341 catccccttc ttctctgtgt agcggccaac gtgttgaact aggcgggtga tgtggcaaca
    17401 gcacgtcacc cgtttgtctt gttcggagat gctgtgatca ttaagcgatt cggcttaccg
    17461 atcttgttgg cgctcggtct gtctatcggt gctgcagcgg cgcatgggac agttccgccg
    17521 cctcccacct cgccgctcga acaggcgctg agccagatca ccagcgggga cgaggccgtc
    17581 cgagatgcgg cgcttcgcgt cgtgatcgaa ctgggcgata gcacattagt ttcgcgactt
    17641 gatgagatcc gtgccaatgc cgaccgcccc gtgcggctag ccattaaacc tgtgatggat
    17701 ctgctcagaa atcgagccaa attggagagt cctgatccag acagtcgtcg atccgcggcg
    17761 acggatctgg gaacctcggg aaggacggtg gcgattccgt ggctcgatca ggcggcggcg
    17821 accgagtcaa acaagtgggt ccggtacacg atgcaagagt ccgccgcact gcttcatctc
    17881 tcaggggatg accaggccgc caaaatctcg gctgttcgca ctctcggaga tctggcgagt
    17941 cagaacgccg tgccggcatt gcaagagttc gtcgctctgg ggagcgcgcc ggaagcaacg
    18001 gaggcgcagc aagctttggc gaaggtggcc agcgtgtcaa ttgagcgcat cgagacctgg
    18061 gctgtctggt caagcgccat ggaaacgatc ttccgcggcg tcagcctgag ttctatcctc
    18121 ttgatcatgt cggtcgggct ggccatcgtg ttcggcctca tgggcgtcat caacatggcc
    18181 catggcgagt tgatgatggt cggcgcgtac gcaaccttca tcacacagga gctcttcaaa
    18241 gcatttctcc cgaccgcgat gttcgattat tatttcgcag ccgcaatgcc gatggcattt
    18301 gtggttgccg ccgcttgcgg gatggtactc gaactgaccg tgatccgctt tttgtacggc
    18361 aggccgttag aaacgatgct ggcgacgtgg ggcatcagtc tcgtgctcat gcaagccgcc
    18421 cgtgtgtatt tcggcgacct gacagccgtc gtggcaccaa gttggctgag cggcggtgtg
    18481 caagtgatgg ttggggtgta tctgccaaac aaccggctgt tcattatcgc gctttcgttg
    18541 gtctgtgtcc tggcaatcta tgccattttg tttcggtccg gcatcgggct gcgcgtccgg
    18601 gcggtcatgc aaaaccgaaa catgagtgcc tgcctgggca ttcccaccag aaaggtcgat
    18661 gcctatacgt ttgcgtttgg gtcgggtctg gcgggcgttg cgggatgggc gttgacgctt
    18721 attggaaatg tcgagccggg gctgggacaa aactatatcg tcgattcctt tatggtcgtc
    18781 gtaaccggcg gagtcggaaa actggcgggc accattgtcg cctcactcgg tatcggcggc
    18841 ctgaacaaac tgctcgaacc gagcctcggg gccgtgtatg gcaaggtgtg cattctcatg
    18901 ctggttattc tcttcttgca gtggcgacca tcggggcttt tcgccattaa agggcgtcac
    18961 gccgatgtat agcggatgat tcgctcgttg gacatgtcga gccacgacca cctcccttca
    19021 gggtcacgtg cagatgtcgg cctcgccttt tgggtcgccg gattcgtctt gctcattctc
    19081 atgcccttat tgaatgtgat gcccgccgaa gattcctggt ttcacctctc ggatttttca
    19141 ctcaatcggt tcgggaaatt cctcgccttt gccatcctcg cggtcgggct tgatttgata
    19201 tgggggtata ccggtgtcct gagtctcgga cagggcgtgt ttttcgggct gggtgcctac
    19261 tgtatgggta tgcacctcat gctgacgatc ggcacggaaa gtgtttacgg caatgagctg
    19321 ccggatttta tggtatggaa ccaggtcaaa tcattgccgc ttttttggaa gccgttctac
    19381 agtttcccca tggcgattgt gggcgcaatc ctggtgccga ccgcttttgc gctgatcttc
    19441 ggatttctgg cgttcaggag tcgcatcagg ggcgtctact ttgcgattat cacccaagcg
    19501 ctggcacttg tggcctggct cgtgttcaat cgtaatgaga cgaacctcgg cgggacgaac
    19561 ggcttgaccg atttcaagca actgctcggc ttccgacttt ccgacccggg tacgcagcgg
    19621 gccttgtatg tcctgaccgt gctctgtctc ggtggcgcct acttgctgtg cttgtggatt
    19681 acccgttcgc gggcaggacg ggtgttgatc gccgtacgag acagcgagcc acgcgtcgtg
    19741 ttctcaggct atacgccggc aaactataag ttatttgttt ttgtcgttgc cgcggcgttg
    19801 gctggactcg ccggaatgct gtatgtcccg caagtcggga ttatcacgcc ggctcaaatc
    19861 ggggtccttc cctccttgga aatggtcatt tgggtggcgg ttggcgggcg cggaacattg
    19921 gcgggagcgg tgcttggggc aatcagcgtc aatcttggcc gcagcattct gaccaattat
    19981 tttccggaac tgtggccgtt tatcctcggt gggctcttcg tcgtggtcgt cctcttgttc
    20041 cccgacgggc tggttggcat catgcgccaa atacaggaac gaatccacaa catgagagtg
    20101 accgccctct ctgccgagga agggctgcga tgagcgaccg cgggtccatt atttatctgg
    20161 aaggggtcgt cgtcgactac gacggcttca aggcgctcaa caacctcaac ttcatcgtca
    20221 actacaacga actgcgtgtc gtcatcggac cgaacggagc gggaaaaacg acgttgctcg
    20281 acgtgatttg cgggaaaaca aggcccgtcg cgggccgggt cattttcggg aaggatcagg
    20341 agctgatcgg taagcgcgag gatgagattg tcaagcttgg gatcggcaga aaatttcagg
    20401 cgccgtcgat ttatgcgaat ctgactgtgt gggaaaatct cgatctctcg ctcaaacggc
    20461 ccagcaaggg ggtattctcc accttgctcg gcacgtcaac cgcagaggaa cgggagaaaa
    20521 tcgccgacac gttgatgacg atcggcttag ccgactacgc aggcatgagg gcgggatcct
    20581 tgtcccacgg tcaaaaacaa tggctcgaaa tcggcatggt catcttgcag gacccctctc
    20641 tcttgttggt ggacgaaccg gtggcgggta tgacggacaa agaaacggaa cagaccggcc
    20701 ggctgttaca atcgttggcc gaaaagcatg cgatcgtcgt gatcgaacat gacatggaat
    20761 tcgtccggca aatcgcccgt atcgtcaccg tcctccatga agggacggtc atttgtgagg
    20821 ggacggttga gaaggttcag tccgacgacc gagtgaggga gatctattta ggcaggcaaa
    20881 aggtggcgca tgggtaagcg cgatgccgac aagaggccag ctgcatgttc atgatgagac
    20941 atgctccttt gaggatcccg cgagccatga catgaagggg ccatgctgga actgaagcag
    21001 atcaacgcct attacggcga aagccacatt ctccgcgatg tcacgttcac ggtttcggat
    21061 ggtgaagtcg tctgcttgat gggccgaaac ggcgtgggaa aaacgacgac tctcaaagtg
    21121 attaccggac tgctgccagc ccgatcggga gacctggtgt ttgatgcaga aaatgtcacg
    21181 aaggtgtcca cggaccggag ggcacgccgc ggcctggcga ttgtgccaca aggtcgtgag
    21241 gtcattccgc atctcacggt gcgcgaaaat ttgcaactcg gtttctgggc tcggtccgat
    21301 tccccgtccg ccgccgtgga aaaggccgcc ttcgatgagg tgtaccactt atttccgaag
    21361 ctcacgcaga ttcttcatcg tcccggcgga gtactgagtg gtggagagca gcagcaattg
    21421 gcgatcggca gagccttgct ctccaatccg aaactgctct tgctggacga gccgaccgaa
    21481 ggcatccagc cttctattgt cgatcagatc gaagacgtca ttctcggctt caagaagtcc
    21541 cgccggttcg cgatcttatt ggttgaacag ggactccatt ttgccgccag attggctgag
    21601 aaatatatcg tcatggccaa aggagcggtg gtggcggccg gcaagagcga tgagttgagc
    21661 gccgaaatgg tgcgacagca tttgacagtt taatgcatga agcttggagt cacggggctc
    21721 tgtgctcgcg gaacgcggca cggaagaatc aggcatgggg tggatggtgt gcctctactc
    21781 ggcgatgagt gaaaccgcat ggtgagcacc cttgtaagca ccatcatcag gcggtgctcg
    21841 cctgtggaaa ccggcgtatt tcggcgcggc tcgctgggat gataaacaga catatgattc
    21901 cgcctctgtt catcatcctg gccgtcatcg gttcgagctc acatgcggca gcctgggacg
    21961 atcttgggca tatgatggtg agcgcgattg cctatgagca gctgacagcc gagacacggc
    22021 agaaagtcac aacattactc aagctcaatc ccaaacatgc gacgtgggta gccgatgttc
    22081 ccgaagatga aaagccgcga atcgcttttc ttcgggcgtc tcgatgggct gatgatatca
    22141 agcttgatcc ggcgtacgag caggacggga gtcaaaatgg caaccgacct tcagggcgag
    22201 aatccgcccg gaacgtcggg tatgaggata gactccaaca taagtattgg cacttcatcg
    22261 atctcccgtt ctctccggat cgcaccgctc tcgtcgggcc ctcaagtcca aacgcacaaa
    22321 cgcaaattgc gctgttccgc cacacgttga catccactgc cagttccgac tctctcaagt
    22381 cgtacgacct cgtctggctc atccatctcg tggccgatgt tcatcaaccc ttacacgcgg
    22441 tctctcgctt tgacaaagca cacatgaacg gggacgacgg aggcaatggt gtcatggtgt
    22501 gtagccctcc ctgtacagca aaagaacgac tgcacgcctt ctgggatact gtcctcgggg
    22561 tgagcatgga tccgacgacc gccatcgaga ccgctaagca attgcccgcc gccgatccgc
    22621 agcttgccgc catcaccgat gaacgtgtct ggatccagga aagcttcgag gccgctcaaa
    22681 tgcacgtcta cgtctcaccc atcgggatag gggttggccc ctttgagttg accgacagct
    22741 ataaggcggt ggcacaacac gtggctggcc agcgcctcgc tctcgcgggg gctcgcctgg
    22801 ccaacctgct caatgaagca ttccgctaag ctgtggtgtc tgaattccaa aaccgcttga
    22861 ggtgatttct cagcatggat catgtgccag aatgcaacgc gggtgactgg cgctcccgta
    22921 cttcatcact cacctcctgt cagttttgac agtatccaag cgtaggcatc agaagtagag
    22981 tctcccccac catacagctc ttggctagaa cagatcatcg agtcgatgga ttgccaccga
    23041 ccgttggcgg aaggagcgga tgaacatgaa aagacaggcc tctcgtaagc tgcggctcat
    23101 cgccaccctc gcgggtgtga ccatgatgct gactggatgg ttctccggct ctgcatcggc
    23161 agcatccgcc agacaaattg atgcgggtgt ggatgacgcc atcgtgaagt tcgaacaaga
    23221 agtcaaaggc ggcaagagtt tcatggagag cagcaaaggg atgctggtgt tcccaacagt
    23281 gctcaaagga ggggccggct ttggaggaga gtatggagag ggggcactcc ggatcggcgg
    23341 caagacggta gattactaca gtatgttgca ggggtcattc gggtttcagc ttggagggga
    23401 gattaagact gtctacctcg tgtttctgga tgaaggagct ctgaaacgat tccgggacag
    23461 cgaaggctgg aaggcggggg tcgacggctc ggtttccctc atcacgttgg gagcaggcgg
    23521 tactatcgat acgaacaatc tgaaagaccc gattgtcgcc ttcgtcttag ggcagaaggg
    23581 cctcatgtac aacctgtcgc tggaagggac gaaattcaca aaacagcata agaaataggc
    23641 gtgcatggtt gttgggcgtg gggcttgtgt gttcacgcat ggtccgagcg gtgctccatt
    23701 ttcttgcccg ttcgagcagt catagggcag gaagcaactg tgtcgtacgg ccgctcgatt
    23761 ctctgcacgc catttgttac tcgacgctat tcgtcacttg cattctcgcc cccggggcgc
    23821 tccttgccga cgcatgggcc gaagacacac caggcccggt cgctcaaagg cggctggaac
    23881 tggaacacat cgcgctcagg ctcaaagaag ccgaaaaggc tgtcgagaag cgcctattgt
    23941 ccgaaagcct ccggctacat ttctacggct atctcgaagg ttcgtacacc cagaacttca
    24001 ataatccgtc caatcgcatc aatcagctcc gcgtcttcga tgtgaactcc aaccaattcc
    24061 ggccgaacct cgctcagttc gtattggaac gggaagcgaa ggcagaggcg acgggttggg
    24121 atcgagtcgg ctttcgtgtg aagttcaatg cggggcgcga ttcagatttc atcggcggga
    24181 tcaacttgag tacctgggcg gacttccagg agttctacgg tcaatatcga gcacctgtcg
    24241 gccgaggact tactctccag gccggccagt tcaacagcgt tgtcggctac gaagtggtcg
    24301 aaagtccaca taacacacat tattcccggt cgtggctctt cgggttaggg cagccattca
    24361 cgacccgcgg ccttcgtgcg acctatgagt tcaacgaaca ggtgtcactg tccgtcggag
    24421 cgatcgccta catcaactct gcgagagcag acacgaattt cgatccgctt gtcgaatatg
    24481 cattggttgt gacgccgacc gagaaggtga gggtgaccgt ctatggattg gccgggccga
    24541 gggacggtcc ttccggcact cctggcggta ccgtgtttct agccggagga tatctcagcg
    24601 ttcaagtcac cgaccacaca tcggcggtca tcgaatccta ttacgccaac caatccaaca
    24661 gcagcacgat caagacaagc aggaatgcgc gctgggatgg agccgcggcc tacgtgattc
    24721 atgatttcac cgatcggtgg ggccttcgtc tgcgcggtga aatcttcgag gatgcaggcg
    24781 gtttcgtcac ctgccaagga acgacagact atcagcccag agccaacgtg tgtttcggcg
    24841 cgacgtccgg cgcgtcagct ccggcagtgg ctcaaaccct ctgggagttc accggcaccg
    24901 ttcaatacaa accgtttgcc tcattcatca cgcgtcttga atatcgctac gacaaatcga
    24961 atcagaatgt ctttcaggtc ggaggacggg cgaccagcta ccagccgacg ctgtctctgg
    25021 atgtgatcta tctcttctga ctggctctgt ggcgggcgag attctcttcc ggcgtggtcg
    25081 catcgcccgg cgccaccgga atcaaagatc tgctgaaccc cggtttggcc aagagcatca
    25141 accaggtgac gaacacaaag gccgaagtca gcacaggcat gccggtgggc tcaagaaata
    25201 tacccagcga tgcccacaca cgcgtggtga cgaggatgcc gatgatcgta tacacgaggc
    25261 acggcagatt cagtacgtag aagactcctc caagcgccat ggcggtcagt gcagcgttat
    25321 agccgaagag cccatcgcgg atcgcttcct catgagcccc ataaaagatc gcgcttcctg
    25381 cagcgagagt cgttcccatc aaggccatga gggcgccaag gcgcgtgttg acggcgatgg
    25441 cgaggaggat gatgattccg gtcatccaat tgtcctggaa gaagatctct gaaatgccca
    25501 ttgtcattcc gtgaaaccag gtgagccagc tgtactccgg tcgggggcct gtaaagtcgg
    25561 ctggtgaagt gggtttgatc gcgttcgaga cgtcaatcgt ggtgaattgt agcaaggcgc
    25621 caaggaagaa ccatgtcgcc aggacaaacg gcatggtcaa agccgggact ttgtgcggtc
    25681 ccaacaacgc gccaaacgcc ggaaggatca ccgacgtgaa gacagcgcag agcacaatgt
    25741 acagccacag atgcaggttg ggcatgttcc cggtggtgaa attggggctc gtgtaggcca
    25801 cgagggcgac ggcgactaat gcgccattaa agccgaaaag accgtcctta atgagaccct
    25861 tgtccgcttt gaacaggacg gcggtggctg tgctgatgat ggtgccgaag agacaggcga
    25921 ggccgtagat ccaggagtta tagaagatgc cggcgagaat cagcgcaccg gtgatcggat
    25981 tgttctgaaa gaccacctgg gcaattccac gcagcaccca gtcgccgaat ccccacaccg
    26041 gatgttcacg aaaccgatcg cgcagtgcca cgcgcgccac ggcgctctcc tttcgaacga
    26101 aggtcgagcg tgcttgacag gcagcgaatc gtagactagc atccgattcc ccgcaacaaa
    26161 attctgtcgg cgtacctagg taaaaaaggg gggctcgatg catttgactc cgcgagaaca
    26221 ggaaaaactg ctgatctaca ccgcaggaca actcgcggcg gatcgcatga agcggggtct
    26281 gaagttgaac catcccgaag ccgttgccta cctgacggca gcggttctcg aaggcattcg
    26341 cgacggcaag acggtgtcgg aactcatgac ctatgggacc accctgttgt cctacgacga
    26401 tgtcatgccg ggcgtgccgg aaatgatcca tgaaatcatg gtcgagggaa cgtttcccga
    26461 cggtaccaag ctcgtcaccg tgcacaatcc cattcgctga gttttcctct cgtccggagg
    26521 acacagaatg ccaaaaacga aacaccgccc cgcaggcaac gctgcaacga aatcggcgag
    26581 ttcgcttgca ggagcgatca aggccggact ggcgaagccg gtgattcccg gcgaaatcct
    26641 gttcgcgccc ggtgacatcg aagccttcaa gggccgcacg accaaggacg tcacggtcaa
    26701 gaacacggcc gaccgcccga ttcaagtcgg atcgcattgc cacttttttg aagtgaaccg
    26761 gggcctcctc tttgatcgcg agcaggcgtt cggctacagg ctttgtatcc cggccgggac
    26821 ggccgctcga ttcgagccgg gcgaagagaa acgggtgacg cttgtcgccc tgggcggtgc
    26881 gcggacggtg caggggatca acgggttgac cgatggaaat ctcgatgatg cgcaggccaa
    26941 ggcccgagcc ttggcgagag ccgccgaaca tggattcatc aagaaaggag gcggccggtg
    27001 aagatgcccc gcaagcagta tgcggatctc ttcggtccca caaccggtga ccgcgtccgc
    27061 ctggccgata ctgacctgct gatcgaaatc gaaaaagact acacttccta cggggacgaa
    27121 gcggtgttcg ggggaggaaa agtgcttcgc gacggcatgg ggcagtcggc ccggatgacc
    27181 aatgccgacg gcgccctcga taccgtgatc accaatgcac tcatcctcga ttattggggc
    27241 atcgtcaagg cggatatcgg catcaagaac ggtcgtatcg tgggggtcgg caaggctgga
    27301 aatcccgata ccatgccgaa cgtctcgccc ggcatggcct gcggggccgg caccgaggcc
    27361 atctcggcgg aaggctgtat cgtcacggcc ggtgcactgg agacacacgt gcacttcatc
    27421 tgcccacaac aatgttggga agcgctctcg gccggcatca cgaccatgat cggaggaggg
    27481 acaggcccgg cgacgggcac gaacgcggta acctctacac ctggtccgtg gaatatccac
    27541 cgcatgttgg aggcagcgga aggcatcccc atcaacttgg gattcaccgg gaagggcaat
    27601 tgttccctgc caggcccctt gaacgaacag atcgaggccg gagccatcgg attaaaagta
    27661 cacgaagact ggggttcaac tccggcagcc atcgacagcg cgctgagcgt ggcggaacgc
    27721 tacgacgtcc aggtcgcctt gcacacggac acgttgaatg aagccggctt cgtcgaagac
    27781 acgatcaagg ccttcaaagg gcggacgatc cactcctatc acaccgaagg ggctggtggc
    27841 ggccatgcgc ccgacgtcat caagttgtgc ggcgaaccga acgtgctccc gtcctcgacc
    27901 aatccgacca tgcccttcac ggtcaacacg ttggatgagc atctcgacat gctcatcgtc
    27961 tgccatcatc tcaataaacg cgtgccggaa gacgtggcgt ttgcggaatc gcgcatacgt
    28021 cgtgaaacga ttgctgcgga ggacatcctg catgacatgg gcgccatcag catgatgtcg
    28081 tcagacaccc aggcgatggg acggatcggt gaattgatca tccgcacatg gcaaacggcc
    28141 cataagatga aattacagcg cggccatttg accgagcgcg gcctccagtc cggcaagggg
    28201 cagaacgaca attttcgtgc aaagcgctat atcgcgaagt acaccatcaa tccggctctg
    28261 gcccatggcg tggcgcatga agtgggttcg ctcgaagtcg gcaaggtcgc ggatttggtc
    28321 atatggaaac cggcgttttt cggcatcaag ccggaaatgg tcctcaagag cggtttcatc
    28381 gcgcaggcgc agatgggcga tcccaatgcc tcaattccca ctcctgaacc gattatcagc
    28441 cgaccgatgt ttgctgcatt cggacgagcg gttgcgagca cctgcctcac cttcgtgtct
    28501 gccgccgcct tggagcggga tgttccccat cagctcggct tgcagcgacg gatcgtggcg
    28561 gtcaaagggt gccgcactgt tcacaagcgc gacctgaaac tgaacgatgc cttgccccag
    28621 atcgaggtcg accctgaaac gtatgtggtg ac
//
LOCUS       DASSCQ010000101        41289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_808533, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000101 DASSCQ010000000
VERSION     DASSCQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 41289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41289
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808533"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFH05_07195"
     CDS             <1..783
                     /locus_tag="VFH05_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET6678034.1"
                     /translation="LAAAMVGGLCSVIGVFVVLRGLAFVGAGTAHAAFAGVALAYLIG
                     WPPLLLAILFGLVTVWITGWVEEKGTMKLDVSIGILYTTTMALAILFIGLMKTYNAEV
                     YGYLFGNVLAVTVEELRTISALSIVVLGIILLFSKELYFISFDQEMAEASGVPARMMF
                     FLLLTLVALTVVVSLKTVGAILVFAMILIPASTAYQLTHSLSTLTWYSMIIGITCAVG
                     GVLLSAVWDIPSGPAIVLLSTFLFFLSVLLSPKRLRHSIGSV"
     gene            896..1579
                     /locus_tag="VFH05_07200"
     CDS             896..1579
                     /locus_tag="VFH05_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6678035.1"
                     /translation="MRSVTQRGVNGKRTAMSVLWMGLLLTGLWVNSADAVEWKMQDGG
                     IEPGKVVLGMRAGFAPVTQSITGNTSTDVGSLVNFQAMYSLNNWLLVGMMVEWERHSI
                     SVERPEFDLGHQDTVSVLPTVELRPVRFGPVSPYVNMSFGVNVNSFGEDRAIAGNRIS
                     PSNNFAWRLGWGADWMITKQFALNGEMAYKRNDGHATITGPGPVGGGRIDDWNATSFG
                     FLFGVKMFF"
     gene            complement(1654..2085)
                     /locus_tag="VFH05_07205"
     CDS             complement(1654..2085)
                     /locus_tag="VFH05_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6678036.1"
                     /translation="MPAALKTAVTFSEKKELEASQLLRLFHQAPWAKDRTLEDAKDML
                     RHTDIAMCAWDGDQLVGFGRVLTDFVYRATIWDVIVDKAYQKQGIGSDIVQRILNHPR
                     LKRVELFWLCTRRPSFYGKLGFSSKEQTGMVWSRSKHARQE"
     gene            complement(2395..2817)
                     /locus_tag="VFH05_07210"
     CDS             complement(2395..2817)
                     /locus_tag="VFH05_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678037.1"
                     /translation="METGNVLDAIGGLTSQLKTYAAKLPAIVHLTVVPTGIKPKPEAV
                     DDYEGIVSRFRHQSAGTAYKGLNDAFVESLEAFEVGKLLGSVQPLLAVLDHLDRMQRD
                     GEIVVEQGDEKRITEYRAGLHKILPGNKPELDGAGRGL"
     gene            complement(2827..3813)
                     /locus_tag="VFH05_07215"
     CDS             complement(2827..3813)
                     /locus_tag="VFH05_07215"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6678038.1"
                     /translation="MITEFVVTAGEQPKRLDVFLVNRERDMSRSALQRLIELGRIRLN
                     DQIVRASQKIKPGDKITMDVPKPEPLQLRGEPIPLEVLFEDEQVLVLNKPAGIVVHPA
                     PGNWTGTLVNALLHYFQTSGGTMSAVGGKERPGLVHRLDKDTSGVMVIAKTDQAHRHL
                     SSQFKQHTITRVYEALVWAIPKKGHGLIELAIGRDAKERKKFSSRTARPKDAVTGYTV
                     SRRFGKAAAHLLLYPQTGRTHQLRVHLASLGHPILGDPTYGGRKVCCVNGLEVPRVML
                     HARTLGFRHPSTGELQQYDRPSPPDMDFLMRQMEACLDNTPARILTEVKTLD"
     gene            3933..4502
                     /locus_tag="VFH05_07220"
     CDS             3933..4502
                     /locus_tag="VFH05_07220"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6678039.1"
                     /translation="MRWVLALGMMMLTAGSFLTASKAADQPGIPPETVADYLHSIIEA
                     DRTFYSIHVVERLQKGGGTKAAEDWRAKKNVLPLPAQFLIEASELAAKTGSKVRYRLI
                     SLWPINPKNAPDGPLERQSLEALREHPERSVMGTVKRGDTTYFQAMYADRAVSKTCIA
                     CHNGHRKSPKKDFTLHEVMGGLIIEIPLE"
     gene            complement(4480..5472)
                     /locus_tag="VFH05_07225"
     CDS             complement(4480..5472)
                     /locus_tag="VFH05_07225"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678040.1"
                     /translation="MGFSYIVMAALLSSSSPISNFETVGEPVPVRALSGDELLRIGDI
                     HDVQNHFPEALTYYEQALQSFRARKQRRGEATALTKIGSVFERQGRRQEAELRLREAL
                     SLFSKFPDSASHADALFLLAKVSFWVGSSEDAGHFLDRAGEQYRRLQRVEALGSVKIL
                     SGLLKVRDAFPDKGLGDLEQALEDARSRQDDEQTVSALLALGDANFILERFEPAKSYY
                     GQSLAFVEQRPQAHIEAGLRFRLAAVHDLAGQPEQGVDSARRAVSLFQSLRNLPGEAA
                     AWSLLASLHQTLRHHQEAEEATEQALSIYRRQQMLVHAIRQSSPSMGVIPKGSR"
     gene            complement(5574..5987)
                     /locus_tag="VFH05_07230"
     CDS             complement(5574..5987)
                     /locus_tag="VFH05_07230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET6678041.1"
                     /translation="MAGKILLEVVTPEKQLLSQQVDEVIAPGSEGEFGVLPGHCHFLS
                     TLRIGELRYRLGDQVHHMAILWGYAEVTPNKVTVMAEIAEKAEDIDVGRAQAAVEQAE
                     RRLKAGGLPSEVKEAEISLEKARLRRKIAERAGKR"
     gene            complement(6003..7445)
                     /gene="atpD"
                     /locus_tag="VFH05_07235"
     CDS             complement(6003..7445)
                     /gene="atpD"
                     /locus_tag="VFH05_07235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET6678042.1"
                     /translation="MSTGKVIQVIGPVVDVEFPPGQLPNIYNAIKVNQEENMAAGKPA
                     VRLTLEVAQHLGENRVRGVAMSSTDGLTRGMDVQDTGAPISVPVGRETLGRLMNVLGE
                     PVDEKGPIKTKKTYPIHRPAPKLEDQETKTEVLETGIKVVDLLEPYSKGGKIGLFGGA
                     GVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQESKVIDPDDFTKSKA
                     ALVYGQMNEPPGARLRVGLTGLTVAEYFRDEENQDVLLFVDNIFRFTQAGSEVSALLG
                     RMPSAVGYQPTLSTEMGALQERITSTKRGSITSVQAIYVPADDLTDPAPATAFAHLDA
                     TTVLSRQLAELGIYPAVDPLDSTSRILDPQILGEEHYKVARGVQSVLQRYKDLQDIIA
                     ILGMDELSEDDKMVVARARKIQRFLSQPFHVAEAFTGAPGKYVKLKDTVRSFREILDG
                     KYDHLPEQAFYMVGPIEEVIAKAEKMGVKL"
     gene            complement(7524..7622)
                     /locus_tag="VFH05_07240"
     CDS             complement(7524..7622)
                     /locus_tag="VFH05_07240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET6678043.1"
                     /translation="MIQKVTLSYNKTGQAAITKELMDIVGGAEALK"
     gene            complement(7628..9148)
                     /gene="atpA"
                     /locus_tag="VFH05_07245"
     CDS             complement(7628..9148)
                     /gene="atpA"
                     /locus_tag="VFH05_07245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET6678044.1"
                     /translation="MQIKADEISAIIKEKIKGFDKQVDVKETGSVIQVGDGIAKVYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGDDVGIKEGDPVKRTGRIAEIPVGDA
                     LIGRVVNAIGQPIDGKGPIKSQHSSRIEVVAPGVNTRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQRGLGVFCIYVAVGQKRSTVARVVKTLEEHHAM
                     DYSMVVSATASDAAPMQFLAPFAGAAIGEYFRDHGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFAQFGSELDKATQMQLARGVRMVELLKQGQYKPMPVADQVISIYAGVNGFLDDVPV
                     DKVQQFEADLLHYAQQHHPDLKKEVAGAGKIDEKLTAKLKEIITTFKQKMGYGAKS"
     gene            complement(9263..9802)
                     /gene="atpH"
                     /locus_tag="VFH05_07250"
     CDS             complement(9263..9802)
                     /gene="atpH"
                     /locus_tag="VFH05_07250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET6678045.1"
                     /translation="MIKTAVARRYAQALFELLDQSNIEAMRRTLHGLGQAMKESSALR
                     HVVSSPAFGVEERNVVLTELGERLGCPPAGKAFFGQLVKKNRVGFLPDIAEAFAKLVD
                     QSKGTQQVTVSSAAALPPAEQDRIKGRLRETLKREVEVTFHTDATHLAGLRIHIGSTV
                     VDSTVRGRLNAMQSLLTRD"
     gene            complement(9931..11013)
                     /gene="mnmA"
                     /locus_tag="VFH05_07255"
     CDS             complement(9931..11013)
                     /gene="mnmA"
                     /locus_tag="VFH05_07255"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET6678046.1"
                     /translation="MKKPTVLLGMSGGVDSSVAAALLVRDGYEVHGVTLQVWEHEDDS
                     VAASKKWQERGCCKIGIARYVAQRLNISYEVVDERQNFRESVIKDFLDGYAGGITPNP
                     CVRCNERVKLRGLIDLAEQRGIQYVATGHYVRVQECGGQYALHRALDEKKDQSYFLYR
                     LNPAWLPRLRFPVGGMQKADVWAEAEALGLPVDELKESQEICFVSHGDYRTFIESEMP
                     DTRRPGDFVDGNGRYLGRHDGIAFYTPGQRRGLGIAAGQRLYVRQVMPVTNTVVLGPE
                     ETLLSSACHIQGLNLFDRSLCTGKTDVAVKIRYATPATAATAEPIDQDRLHLAFHVPQ
                     RALSPGQSAVLYHGDRVLGGGMIQAI"
     gene            complement(11088..11486)
                     /locus_tag="VFH05_07260"
     CDS             complement(11088..11486)
                     /locus_tag="VFH05_07260"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6678047.1"
                     /translation="MWARKDQVEHIEPDFENFTLLAKDVDFKGVVNFDGTVRVDGRVE
                     GEIHTTGTLIVGEHAMIKGIVSAGVLMNSGKINGTITAVEKIHILKPGVLVGDIRTPL
                     IAIEEGSHFHGMCDMGVQSWSEPAAVSIAS"
     gene            complement(11533..12009)
                     /locus_tag="VFH05_07265"
     CDS             complement(11533..12009)
                     /locus_tag="VFH05_07265"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET6678048.1"
                     /translation="MWGQDKQEGKRAVEGENEEGTEPVSRASEGTEDIIAFVGKGVEF
                     KGVISYSGTVRIDGFLDGEIHTDGVLLVGDEAVIQAKVTAGTIVCKGKITGDIAAKER
                     VKLRAPAVFNGSMKTPMLSIEDGVLFNGGLEMSQGMRDGTALHQIGTPPAAVKRVS"
     gene            complement(12168..13016)
                     /locus_tag="VFH05_07270"
     CDS             complement(12168..13016)
                     /locus_tag="VFH05_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET6678049.1"
                     /translation="MEKKALGRGLDALLPTTKPAPMPELPEVQHLRIDAIVPNRYQPR
                     QTFPPQELAELAASLKQSGLLQPVLVRRKGDGMYELISGERRWRAAREAGLETIQAMI
                     RNCSDEESILLALVENLQREDLNPIEMARAYQRMMNEFGLTQDIVAQRVGCERSSVAN
                     AVRLLQLPVEVQHLIETDQLSAGHAKVILGLASPAEQLRVAHLAVSRHLSVRETEHIL
                     SSGLIARKRHAKELRRSPFSDVEERLQKRLGTKVSVLNGRRGGKVIIHYFSSAELDGL
                     LEQLLQ"
     gene            complement(12994..13770)
                     /locus_tag="VFH05_07275"
     CDS             complement(12994..13770)
                     /locus_tag="VFH05_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6678050.1"
                     /translation="MALIVAVANQKGGVGKTTTSVNLAAGLALQGKRTLLVDMDPQGN
                     ATSGLGIDPRSLDKTVYNCLINYVKFDETVRKTEVSGLLLLPANSDLAGAEIELVTVE
                     EREKLLRSFLSGVESLFDIIFLDCPPALGILTVNALVAARSVLIPVQCEYYAMEGLSR
                     LIGSIERIQQSWNPGLTIEGIVLTMYDPRNTLARQVAEQVRGHFKDLVYQTTIPRNVT
                     LAEAPSYGRPVLLYNMASAGAQAYQSLAKEFINHGEKGPR"
     gene            complement(13808..14470)
                     /gene="rsmG"
                     /locus_tag="VFH05_07280"
     CDS             complement(13808..14470)
                     /gene="rsmG"
                     /locus_tag="VFH05_07280"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET6678051.1"
                     /translation="MEQERELRQFLQAAAQTLGLSLTNTQIEQFWTYLAQLLRWNSTI
                     NLTSITDPYEIIGKHFVDSLAALVAVDFPIEGVVIDVGSGGGFPGIPLKIVRQDLRLV
                     LIEPTQKKSSFLRAVVGTLQLKNVCIFSGTIGEYAVEEHHTLGDLMVVRALRFDQVAP
                     VAPSVLKPIGKVVLYGTGRGDSEAGLATSSSFTIKSQYPFSLPRNYGTRVLTILSQAV
                     AA"
     gene            complement(14527..16413)
                     /gene="mnmG"
                     /locus_tag="VFH05_07285"
     CDS             complement(14527..16413)
                     /gene="mnmG"
                     /locus_tag="VFH05_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET6678052.1"
                     /translation="MSRSFDVIVVGGGHAGCEAALAAARMGAQTLLLTMERDRVAQMS
                     CNPAIGGIAKGHLVKEIDALGGEMAINTDRAGIQFRFINTSKGPAVRALRAQCDKKMY
                     REAMRRSLEGQPGLTIGQGTVDRLLLKGHTICGVVTDCGERLGARAVIITSGTFLKGL
                     IHIGLNHFPAGRAGEVSAEHLSDCMRDLGFEIGRLKTGTPPRLDRDTIDFSAMIPQPG
                     DDPPPPFSYRTDRIRVRQMDCYLTYTNAVTHDIIRGNLDRSPLYSGVIESVGPRYCPS
                     IEDKVVRFAEKTRHQIFVEPEGLDTPEFYPNGISTSLPVDVQMAMLKTIPGLEQARML
                     KPGYAIEYDYFPPRQLHPTLETKLVEGLYHAGQINGTSGYEEAAAQGLMAGVNAVLKL
                     RERPPLVLDRSEAYIGVLIDDLITKDAHEPYRMFTSRAEYRLLLRHGNADLRLTEIGR
                     QVGLISDDIYSKFCVKQKQIDDEVSRLEGSRLHVTDDIRQRLMDAKLGEVGGPCSLAQ
                     LLRRQGVDYETLLDCLGLDRHHNPEIIEEIEIQIKYQGYIKRQMQQIGRFKKLETRAI
                     PNAFPYDAIRGFSQEVREKLQRVRPASIGQASRISGVTPAAISLLIVALEKYKEQSAA
                     QAIP"
     gene            complement(16428..17852)
                     /gene="mnmE"
                     /locus_tag="VFH05_07290"
     CDS             complement(16428..17852)
                     /gene="mnmE"
                     /locus_tag="VFH05_07290"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET6678053.1"
                     /translation="MLRGGPEDTICAIATPAGEGGIGIVRLSGSQALRIADKFVQLRS
                     GQPLASVPSHTLCLADLRFPDMNQTVAQDPSIPKPRECFDEALVVYMKAPRSFTGEDI
                     VEIQSHGGSLILSLTCRACLEAGARLAEPGEFTKRAFLNGRLDLSQAEAVLDTIRAKS
                     AASLTAAQRQLRGDLAREVDTAREALVHLLAQLEAGIDFGDEDVDIIHKQDLVGTVDR
                     VARLVGRLRDTARAGRLLRDGARVVIAGRPNVGKSSLLNRLLKEERAIVTAVPGTTRD
                     LIEEAIDLGGVLVHLVDTAGIRDTEDPVEHEGIKRSWSAQEQADLRVIVLDGSLPLTA
                     DDRRLMDQAAEMRHVIVINKADLPAQVSPTSVNPGSVCIAVSAKTGDGVEDLRLAIRA
                     QLTGSGPETADGVLVTNVRHQTALTRAHESLVEATSSIKDEMAAELVAVDLRAAADAL
                     GEITGAITNEEILSRIFSTFCIGK"
     gene            complement(17865..19529)
                     /gene="yidC"
                     /locus_tag="VFH05_07295"
     CDS             complement(17865..19529)
                     /gene="yidC"
                     /locus_tag="VFH05_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET6678054.1"
                     /translation="MEKRVVIFLVLSLAVILGYDLLLKQFGLLPATPTVQEPGGQEAA
                     APRGQHETPSAETTASAPRASSSASDTASSGDSRPSLPPTERTVTVETDFIRVAFNAR
                     GGVISSWELKRHYTSATDGNPVQLVYQGAKFKEPLSITTSDVAADKALREGLYAIETD
                     FTTLDDAHPTGHVTLRFHDAASGLKVQKRLTFRQENYLVDVSLTVDGLSGAYDIVLGT
                     NFGIVEWGDGFIGLIGSASLVDGKVEKETPDLELERKGSVQWVALQDKYFLSVLMPQG
                     ASAALAKKEGDKLVSAGVRLPASGARTTDLQLYAGPKEYDTLRSLNVGLEETIDFGWF
                     IFGSWSVVKAVAKPIFYVLRFINEYTHNYGVTIILLTMGIKMLFVPLQYKSYKSMKQM
                     QLVQPKVLALQEKYKDDRDKLNKELIKLYRDHKVNPVGGCLPMVLQMPVFVALFNILY
                     MTIDLRQAPFVGWITDLSVQDPYYVLPIIMGATMVIQQKITPTTMDPTQAKIMLVLPV
                     FMTFLFVNFPAGLVLYWLTNNVLTIGQQVLTDRLFAIRPLVTDPSK"
     gene            complement(19531..19770)
                     /gene="yidD"
                     /locus_tag="VFH05_07300"
     CDS             complement(19531..19770)
                     /gene="yidD"
                     /locus_tag="VFH05_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET6678055.1"
                     /translation="MKTMCVTLLNGYRFWISPWLGPACRFDPTCSDYAGQAIERYGVI
                     RGSGLAVWRLLKCHPFHSGGHDPLMPEVTTRGTAE"
     gene            19799..20131
                     /locus_tag="VFH05_07305"
     CDS             19799..20131
                     /locus_tag="VFH05_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678056.1"
                     /translation="MTPQRSLPQAFQFLERKCKGVSLWKYEQGMIGGQLVSNLSRQLP
                     EHTLDPIATNGNAEPSPHNDADAARAAFGLAHQQVEAGCGQPPAMLFHKLNVAARAKK
                     KDSISCPP"
     gene            complement(20287..20424)
                     /gene="rpmH"
                     /locus_tag="VFH05_07310"
     CDS             complement(20287..20424)
                     /gene="rpmH"
                     /locus_tag="VFH05_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET6678057.1"
                     /translation="MSFTFQQPSNLKKKREHGFRKRMSTKNGRKVLARRRAKGRARLT
                     V"
     gene            20814..21425
                     /locus_tag="VFH05_07315"
     CDS             20814..21425
                     /locus_tag="VFH05_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678058.1"
                     /translation="MNMRHVLVAVLSAWVLAAGCAQPPADQINAAEQAVKDAQQSGAA
                     TYAANDYAKVEGLLTAMKQEVADQDAKFALLRDYGKAEQLAATTKAEAERVKADATKK
                     MEEAKFAATQAQHGAEAAVQSTLELVSRAPAGKDRAALESIKTDAQALKASLNDVQLM
                     LDKSDYLGAHTKAKAIREKAEAVSHEVQTALAKIGKGTASKKK"
     gene            21432..22583
                     /locus_tag="VFH05_07320"
     CDS             21432..22583
                     /locus_tag="VFH05_07320"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6678059.1"
                     /translation="MREMPLRHAVRIVCYSSMFALCGSLAACVESVPSDVVQAIESVD
                     NDLAQLRAAELAPSAYNAFAYQWVTLRSRVEAEEDTVRWPWESNDLEAALRGLHAEGT
                     RTVALLTEQQETLRRSAARQLAEVEAQAHVLTTHVGAIDSRLILGEKPIETDLLLKQA
                     RTFYEQRRYEQSLSASQRAFHYLAAQSALLQRELGRYASPDRVARWQRMAKETIEWSR
                     THKRAAIVVSKAERSLTLYRNGQKVVSYPVRLGFNGIKEKRYQGDGATPEGRYRILSK
                     RGRGQTQFYRALLLDYPNQDDRRRFLSERKMGNIPMLRGIGGQIEIHGVENELMAQTL
                     GCVMLENVQMAALFDRVDAGTPVTIVGALLERNSVAKALASLSGQRNEI"
     gene            22590..23219
                     /locus_tag="VFH05_07325"
     CDS             22590..23219
                     /locus_tag="VFH05_07325"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6678060.1"
                     /translation="MPRIVASLCLGLIGLVLLTLAQSSSLTVTDASIPAVIPDPATLH
                     ALQTRYRSLSKQLNHLMPSQPYIVVDTARNHLYIKRRDHVMLDALASTGSGTILDKPG
                     EAKGQWVFDTPRGEFTVQSKLINPVWVKPDWAFVEEGLAIPKSYTDRIEPGVLGEYAL
                     GFGKGFFIHGTLYTRLLGKNVTHGCIRLNDDDLKSVYRLAKVGTPIMIF"
     gene            23224..23880
                     /locus_tag="VFH05_07330"
     CDS             23224..23880
                     /locus_tag="VFH05_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678061.1"
                     /translation="MPRPSVTGILICFFVLTAAPPTRAVDADYPLSVDVTNPAALQEA
                     TRILAEELKLASRPQAYVIVDLIDRAVLIKGRGVELHRFSIEHWSAGQLADASTTFRL
                     QTRPPVTRRKIEPAAGGEQPPISLDDMPTDFTLQFSPPFSLTVHPSASDDIGRWLRSK
                     GREWWMWLKVWAMTLATGREPLPQPWLHLTVTSDHAQSLAWTVTEGMAFLIRRPPPSP
                     "
     gene            24101..24883
                     /gene="pgl"
                     /locus_tag="VFH05_07335"
     CDS             24101..24883
                     /gene="pgl"
                     /locus_tag="VFH05_07335"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET6678062.1"
                     /translation="MSPPAEIRIAQDSFTWATEAATLTQELRRQAVKERGLFLLALSG
                     GRTPEQLYRRLASSPFCDQGDWTNTEFFFSDERCVPAHHPDSNFNLADETLFRPLDIP
                     SHRIHRMACEQADADLAAHDYEQVLRTVTKTRPEGWPRLDLVLLGIGQDGHTASLFPG
                     TDAVQERRRCVTVGYAPSGPRTRLTLTLPVINQATVVLFLVTGTAKAEIVKTILEPQQ
                     EVDRLLPAALVRPEGGRLIWLLDPPAAAQLTGHLESNSPRYL"
     gene            24880..25935
                     /gene="glk"
                     /locus_tag="VFH05_07340"
     CDS             24880..25935
                     /gene="glk"
                     /locus_tag="VFH05_07340"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET6678063.1"
                     /translation="MILAGDIGGTKTHLALFDWTTERVDPLRLETFHSADYASLEDML
                     EEFLAPPKPPTPIDILESRPQDEPSSPPQDELPPIRAACFGVAGPVIQNHSRTTNLPW
                     VVDGAALAKRFELPAVQLLNDLEAMAHGIVLLHADEVVTLNHGTPPVQKQAIALIAAG
                     TGLGESILFWDGTRYKPLPSEGGHADFAPNSDHEIELLRYIRSQYLHVSYERVLSGPG
                     LHAIYEFLRDAKRNEPTWLAEKIKAGDPAAEIAEAGLKGQAEIAKQALDLFASIYGAE
                     AGNLALKAMAINGVYVGGGIAPKLLAKLQDGTFMKAFTNKGRYKRLMGTIPVHVVMNQ
                     HTGLLGAASTAARLAQA"
     gene            25932..26630
                     /gene="rpiA"
                     /locus_tag="VFH05_07345"
     CDS             25932..26630
                     /gene="rpiA"
                     /locus_tag="VFH05_07345"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET6678064.1"
                     /translation="MSTSDLDTLKHAAAAKAVEFVKDGMVLGLGTGSTAKHLIMALGE
                     KVKAGMNLSGVSTSQETAMLARQCGIALIDAEACWTIDVAFDGADQVDPAFNLIKGGG
                     GALLKEKIVAASAMKFVVMVDFTKRVTVLGGAFPLPVEVVPFGWRNTARKIEELTRSR
                     VVLREREGARFQTESGNLIVDVHLARIDRPDELETALHRIPGVVEAGLFVGRTDVLIV
                     GTPDRTEIHHVKRG"
     gene            26773..29376
                     /locus_tag="VFH05_07350"
     CDS             26773..29376
                     /locus_tag="VFH05_07350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET6678065.1"
                     /translation="MNEVPHGLDDPYRLPQHVRPTRYHLILEPDLVSATFQGRATIAL
                     TVLKETRVIVCNALELAIGEAVVEAGGRRQTASVTLDESLQRCRMTVPEPLAPGAWQL
                     HVGFRGILNDKLRGFYRSTYTDQRGSQQTIAATQFEATDARRAFPCWDEPSFKAVFST
                     TLVIDENLTAVSNSLVVSETREKGKKIVRFADTIKMSTYLVAFVVGRMEGSAPSVVGN
                     TRLQLWTVPGKTHLTSFGQDIAAASLHFFERYYDIPYPGDKLDLLAIPDFASGAMENL
                     GAITFRETALLVDQRTGTHGELQRVADVVAHENAHMWFGDLVTMSWWNGLWLNEAFAT
                     FMEMLAVDAWKPEWKRWDAFGASRGAAFAVDGLHSTRPIEFPVRAPKDAEAMFDVLTY
                     EKGASVLRMLEQHIGPTVFRDGVRDYLRTHAYGNADTNDLWVSIGKASNQAVPDLMNG
                     WIFQPGYPLVTAELTKECEVVLSQQRFSYLPDPPPASWQVPVQLRVQTGAGGESRRVL
                     LTGTRLNVSCPPDTQFILVNEGGHGFYRVRYGPNLLKRVLEAGLEKLAPIERLNLVND
                     AWATTIAGLMPLSDYLDLTGRFALERDKNVWAVLLDSFAFLNRIIEPADRAGLESFVR
                     SRVGEAVAAVGWTTGPGDNDLMRQLRGELIGALGRLGNDQATQARATELYAMVRKDPA
                     AIDPNLLPALVSILAFIGDAARYEDFRARFKTAATPQEERRYLFSLAMFQQHALLGRT
                     LDMTLNGEIRLQDAPFMVSAVMNNVYGREQGWRFVKMNWDLLDRRFPKQGLRRMCGGI
                     TGFCKPELERDVREFFQARQVDLGGKTLEQYLEQLRIGVSFAERSRRDLQEFLRRNQY
                     SPEVAQTSVPG"
     gene            29408..30601
                     /locus_tag="VFH05_07355"
     CDS             29408..30601
                     /locus_tag="VFH05_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6678066.1"
                     /translation="MSSRAGSICFFGVMLCALMAACNGSDEVGGGGAGPPPATSNSLR
                     LQTVTAVLSSPVFLTAPTGDVGRLFIVEQGGLIRILNSLDGTPRATPFLDVAGLIVTG
                     GEQGLLGMAFDPNYAGNGRFYIYYTNTAGDIVIARYGVSTNPDIANAGAQAILKTIAH
                     PTNQNHNGGMLAFGPDGCLYAGIGDGGGSGDPNGNAQNTNSLLGKILRLDPETGSACG
                     TDNPFANGTGGAPEVWSFGLRNPWRFSFDRSTGVLYIGDVGQDQREEVDAVVGPNAGQ
                     GVNFGWNIMEGFACFNPPSGCNSSGLTPPILDYSHDAGACSVTGGYVYRGTLNPAVNG
                     SYFYADYCAGFVRSFQLQGGRPSSQNTWPLLSPGGQITSFGEDARGELYILTQTGGLS
                     RIVAN"
     gene            complement(30638..33295)
                     /locus_tag="VFH05_07360"
     CDS             complement(30638..33295)
                     /locus_tag="VFH05_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6678067.1"
                     /translation="MNWFMFSMAWRETRGAWRHFVYFFVCIAVGVSALVGVSLFATHV
                     DDAVSKQAKGLLGGDLEIRSSHPLTEESRAIVDSLHQRAVDTTHVTELVAMAAHPNTA
                     GSGHTTQIVELKAVDPGYPLYGLLKVEPALPLERLLDPDSTACGGCHGVLVQEALLLR
                     MGIRVGDRLKLGKAEFLITGLVRTEPDRMANAFSLGPRVIVSQAGLRSAGLVQRGSRV
                     RERQLLRLPSGMPPEPLRAELRGRLAADHVRISGYRDAQPQLKQFLEQLARYLGLIGL
                     TALFIGGLGVAMSIHAFMQEKMKTIAILKTLGADSAVLIKVYMVQAIGLGLIGSGVGI
                     VLGILLQRWLPPLLAGAFVSDLLDQLGVSAELSFVSIGPLLKGGAMGLLTTVLFALWP
                     LLRVREIRPAAIFRREVEGGIPDEHNRRRTWWIRWGINGEAPVAIGAAILAGLGLLSI
                     WQAGSWKVGGLFIGGLAIAMLTLFLCARVLIRALSLVPRPASLSLRYAVGNLLRPGGH
                     TTGVLVAIGISVMVIMTVSLVEHALLRQVGDNRPVDAPTFFFIDLQVDQTEGFAKLVQ
                     QLTRGRQPEMTPLVRSRLHTVNGEVVKVEEEADHEERRDENKEERRKQWYLSREYVLT
                     FLDQLPKGNDVVAGTWWQPGQPFSRPQVSIEEEAAKSLGLVVGSQVELDIQGATITAE
                     VSSIRKVDWGNFSTNFYMILSPGSLDGAPLTYVATIHVDPEQEVPLQQAVVAAFPNVS
                     AIHVGDVLRNFASVLDRLALAIRAVAMFCVMTGSLVLAAALAATRYRRLYESVVLKAL
                     GATRSLIAGIFALEYGLLGVVGGILAAVLSSALSWLVLTYVFDLSWGLHPMVLVTGLL
                     CTMVLTLVVGLLSTFRILGQRPLAILRHE"
     gene            complement(33292..33996)
                     /locus_tag="VFH05_07365"
     CDS             complement(33292..33996)
                     /locus_tag="VFH05_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6678068.1"
                     /translation="MIAIRHLSMRLTAGGRRVTILDDVALSIPPKQMVAVMGPSGSGK
                     STLLGLIAGLDRPTEGTIVLDGIDITTLSESRMAKFRRMKIGYIFQSFHLIPTLTALE
                     NVLVPLELAGHAAAKDLAAELLHAVGLQDRMHHYPVQLSGGEQQRVAVARAFACRPPI
                     LLADEPTGNLDSQTGRHVMDLLLSLHRDYGTTLVLVTHDRAVASLMHRVIALRDGRIE
                     SDDLISETESHQEPTL"
     gene            34047..34709
                     /locus_tag="VFH05_07370"
     CDS             34047..34709
                     /locus_tag="VFH05_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HET6678069.1"
                     /translation="MTLRALAQAFAASFITLFLATTSPSITHSAGGGSNNDRPRIVAF
                     GDSLTAGLGVGAEETYPAQLQRRLDSRGLQYRVINAGVSGDTTAGGLRRVDWVLKSRP
                     DIVILELGANDGLRGLKLQETKANLEKIIQRCRDASAVVVLAGMKLPPNYGTEYTTSF
                     ESIYPALAKQFRLPLIPFFLEGVAGSTSLNQPDGIHPTSEGYRIIADLVMETVTPLLV
                     RE"
     gene            complement(34781..34969)
                     /locus_tag="VFH05_07375"
     CDS             complement(34781..34969)
                     /locus_tag="VFH05_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678070.1"
                     /translation="MIKMWGRVSAAAGMLVIVGASSVLANEPKGGAPDYSGISTMYYA
                     LIGLVLAYGVYDTFFKKS"
     gene            complement(35077..35961)
                     /locus_tag="VFH05_07380"
     CDS             complement(35077..35961)
                     /locus_tag="VFH05_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678071.1"
                     /translation="MRNKIPCSAERLSLLDPPMIQGVVDRNAFHLGSQYLSENRVRIV
                     EANEAQISSAVIGNSGLYEQTIRLKDGHLVSRCSCSLPEEPMCRHCIAVLLEYHRWVQ
                     PRTKKARQNPVPMSSEASNGNTVPPSNFTADLKLSEVMAFIEWLNPTMKALEKGEPLP
                     EQSHLSGDLAAWVQTIRTLDERRRESEEIQLTLESEQREREAYVERMTQQLQASMAEV
                     KAAQSQLQHLQQEVAAYQDMFVKVAEIASEVGGYDSQMKTIAGELISKGTQLDKLANS
                     FREVAVALKTIAKPGSTP"
     gene            complement(36081..37049)
                     /locus_tag="VFH05_07385"
     CDS             complement(36081..37049)
                     /locus_tag="VFH05_07385"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HET6678072.1"
                     /translation="MIGHGLLIGLLWMTAGCAATDASVSPTPPSTEFREGQIIDTANG
                     QFISLEQLVSHLLQQDVVYLGEEHHNRFHINSALSVLNGLSAAGRRPVLAMEMFGWDA
                     QPLLDRYVGGAELSRQEFLSQVGWSQNWGGAFETYEPLVAFAKHERLGLVALNPPKSL
                     VRLVAKEGLDHAREEADWKRWKMQDETIVDDPLYRQRIFDQLRACHGGGSDDMYRTMY
                     EASMVRDEGMARTVVALVESIRERSDALAGPVVSYTGGGHVQYNLPVANRVVRRLSGN
                     VRQTSLYMTSYERGRQDDVREMLDGKIADYVWLTPVSGEGMPRRCR"
     gene            37211..38524
                     /locus_tag="VFH05_07390"
     CDS             37211..38524
                     /locus_tag="VFH05_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678073.1"
                     /translation="MLSHAQLLAAFRSQITRLLQERDTQWERSRRLIEAASFSGTVRS
                     LVERAQHTDLPAPVRDALLTALNEGQVARIQDLSGPRLKELTGLPPSKALRALCVWFG
                     LVEDAPSRWPVSSLDGESVATFLHTHSNPFDLLVTADVPSVVEFGAGDLSFATELADL
                     YAPRICEHNRTLLLHCIDRLSPQSKLGGPLHPSQDRLQALRSRTDVSFQFFGDQDMCG
                     FDELAEAGRLASRYAVAVCWAPATPTFAYEPTRLSPEVIERELVRTKGPYRHAQYAGE
                     PALEVHNGDRTLLFPPWKFDIHGPLVLLNLLARGGQLGVLGAVDNQVFWELLAQLLED
                     ERYRPENEPFTPENLSTIFNPVYEPLATLPIGESLELASCGTLRRSIPRVLPSKRLPE
                     TYRFRSVLIRRGACFPGMPASHTARRFSDMVEEASPWMMTLAPAV"
     gene            38655..39596
                     /locus_tag="VFH05_07395"
     CDS             38655..39596
                     /locus_tag="VFH05_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6678074.1"
                     /translation="MNVPKPFHAIQDNIARVIKGKAQVIEMAVVCLVARGHLLLEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGVSIFNRQHQAFEFVPGPIFANIV
                     LADEINRTTPKTQSSLLEAMSEAQVSVDNQTHSLKQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPSLEEERKVLNRPYALHPADSLAPVLTAQDVLELQRRIEEVHMDE
                     SVCEYLLAIVHATRQSELLSLGVSTRGALALNNASKALALVRGREYCLPDDVKELAPI
                     VLSHRVMLSRAQGMRAQSFEQAERIVLDIVETIPVPV"
     gene            39617..40762
                     /locus_tag="VFH05_07400"
     CDS             39617..40762
                     /locus_tag="VFH05_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6678075.1"
                     /translation="MTKAFGHNPALRIRSRLHYLATRRSIRLTSEGIRFLLFTIGIGV
                     AAINTGNNLFYLLLAMMLGLVVISGLLSEHCLRRLEFHRHLPDLIMANEPTTVTLSVT
                     NHNRWLPSFSLRLSDIVSGQQLDRGLFIDHLAAQRSVLLSYPIVATTRGKLSLDCVRA
                     ETLFPFGLFLKRALYQVPAEIPVSPPIKPVSLRFIDEMVSEGQGLSLPRRGHGTQLYN
                     LRLYQPGDDSRAIHWMTTARTSTLMVRETEAEDQRRMTIALSTLAPDERNTLFEHSVT
                     LVASLLWHLSQRSFPLRLIVHEADSGLGCGSDHLFAMLRLLAVCERRAPDSTGPTVSN
                     PILTLGHDAERGYTVVVLPWSDPEASAAVSADRVLQAAHIEGLTNGF"
     gene            40752..>41289
                     /locus_tag="VFH05_07405"
     CDS             40752..>41289
                     /locus_tag="VFH05_07405"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HET6678076.1"
                     /translation="MVFNQACRFSAIVLAATSFAGLLFARSMPSWLAILTVTVLVFAI
                     LQTFGWPSGNTVLSGLPNAPSVWNSLLIGAFVLFLLDLTMISRELLPAGIHFLVVLLN
                     VKLMTLHDRRDYRHLYAISLMAILASAALTTDVWYVSIFVFYLLAVVWSLLLHHLTSG
                     PPSANPARAQVSPRATWFI"
BASE COUNT         8775 a        12145 c        11473 g         8896 t
ORIGIN      
        1 cttgccgcag cgatggtcgg agggctctgc tcggtgatcg gcgtctttgt cgtcttgcgc
       61 ggactcgcct tcgtcggggc agggacggcg cacgcggcat tcgcgggcgt ggccttggcc
      121 tatttgattg gctggccgcc gttgctgctc gcaattctct tcggactcgt gaccgtctgg
      181 atcacaggct gggtcgaaga gaaagggacg atgaagctgg atgtgtcgat cgggattttg
      241 tacaccacga caatggcgct ggcgattctc ttcatcggct tgatgaaaac gtacaacgcc
      301 gaggtgtatg gctacctctt cgggaacgta ctcgccgtga ccgttgagga actgagaacg
      361 atctctgctt tgagcattgt cgtgttgggc atcattctgt tgttctcgaa agaattgtat
      421 ttcatctcgt tcgatcagga aatggccgag gcgtccggcg tgccggccag gatgatgttt
      481 ttcttgctgc tgactctcgt tgccttaacg gtggtcgtat ccctcaaaac tgtcggtgcg
      541 attttagtgt ttgccatgat actgatccct gcttcgaccg cctaccagtt gacacatagc
      601 ctctcgacac tgacgtggta ctccatgatc attggaatca cgtgtgcggt gggaggcgtg
      661 ttgctctcgg cggtatggga catcccgtct gggcccgcca tcgtgctcct atccacattc
      721 ctgttcttcc tgtcggtact cctctcgccg aaacgactac ggcattccat tggcagcgtg
      781 taaacagtgc tggcagaatt tgcttgcctt cggactaggg ttcaacgagt attgatttta
      841 cgtagctgaa aggagataag taggtacatt cgtcattctt atgacaggag gtgctatgag
      901 gtcggtgaca cagcgtgggg tgaacggaaa gcgtactgca atgagcgtcc tatggatggg
      961 cctgctgctc acgggtctat gggtgaactc cgccgatgcc gtcgagtgga aaatgcaaga
     1021 cggcggcatt gaaccaggga aggttgtcct gggtatgaga gcaggatttg cgcccgtgac
     1081 tcaatccatt accggtaaca catcgaccga cgtaggttct ctggtgaatt tccaggccat
     1141 gtacagcctc aacaactggc tcctggtcgg catgatggtg gaatgggaac gtcacagtat
     1201 cagcgtcgag cggccggaat ttgatctcgg ccaccaagat accgtatcgg tattgcccac
     1261 agtcgagttg cgccctgttc ggttcggccc cgtcagtccc tacgtcaaca tgagttttgg
     1321 cgtgaacgtg aatagcttcg gagaggacag agctatcgcg ggaaaccgca tttcaccgag
     1381 taataatttt gcctggcgcc ttggatgggg agccgactgg atgatcacga aacaattcgc
     1441 attgaatgga gaaatggcct acaagcgcaa tgacgggcac gcaacgataa ctggtcccgg
     1501 ccctgtcggt gggggccgca ttgatgattg gaatgcgact tcgtttggat tcttgtttgg
     1561 cgtaaaaatg ttcttctgat ggtctactga gtctgcgcag agtcggcttt acgcagctct
     1621 gcgcagatca cacgaggaga gagaaccgga gcgttattcc tgcctggcat gcttcgagcg
     1681 gctccaaacc attcccgtct gctctttaga gctaaaaccg agtttaccgt agaacgacgg
     1741 acgacgcgtg cacagccaaa acagctcgac gcgtttgagc cgaggatggt tgagaatgcg
     1801 ctgaacgatg tctgagccga tgccttgctt ttggtatgcc ttgtcgacga taacgtccca
     1861 gatcgtggct cgatacacaa aatcggtcag cacgcggccg aaccccacga gttgatcacc
     1921 atcccaggcg cacatggcga tgtcagtatg gcgcagcata tccttggcat cctccagggt
     1981 gcgatccttc gcccaggggg cctgatgaaa aagccgcaac agctgtgatg cttcaagttc
     2041 tttcttctcg gagaatgtaa cagcggtctt gagggcggcg ggcatcttat actagagtat
     2101 cctcgcaact caggggatga tgggccttag tgtacgagga atgcgatggc aacgcaagtc
     2161 cgtagctgcc ccaagtgctt ccaactcatg tggctcacgc acaaccagta cgaattgctt
     2221 gaccaggaaa ccgttcgagc gaagtgcccg cactgtggat caaccgttcg attcaaattg
     2281 gtgtcggaag gcgccaacgc gactggcccg aaaatggggc attgatcgga gcatcgtccg
     2341 ccgatcgtgc gtacctctaa cgaggagggc tcaggaccgc gtcgacacgg caggttacaa
     2401 accccgtcca gcaccgtcca attcaggctt gttccccggc aaaattttgt ggaggccggc
     2461 gcgatattca gtgatgcgct tttcgtcgcc ctgctcgacg acgatctccc cgtccctctg
     2521 catccggtcc aggtgatcga gcaccgcaag taacggctgg acggaaccca ggagttttcc
     2581 cacttcaaag gcttccaatg attcaacgaa tgcgtcgttc agtcctttat acgccgttcc
     2641 ggcgctttga tggcggaaac gggaaacgat cccctcatag tcatcgaccg cttcgggctt
     2701 gggcttgata cctgttggaa cgaccgtcaa atgcacgatg gcgggcaatt tggccgcgta
     2761 ggtctttaac tgcgacgtga gaccaccgat cgcatcaagg acattgcctg tttccatacc
     2821 atcgtcctaa tcgagtgtct tcacttctgt caagattcgg gctggcgtgt tgtcgagaca
     2881 tgcttccatc tgtcgcataa ggaagtccat gtcaggcggg gagggtcggt catactgctg
     2941 gagctcaccg gtcgaagggt gacgaaaccc caatgttctg gcatgaagca tcacgcgagg
     3001 aacctccaat ccgttcacgc agcaaacctt tcgacctccg taggtcgggt cgccgaggat
     3061 gggatggccc agcgaggcca gatgtacacg caattgatgc gtccgtcccg tttgaggata
     3121 caacagtaga tgggcagcgg ccttgccaaa acgccggctc accgtgtacc cggtcaccgc
     3181 gtccttgggc cgggccgtgc gagaggaaaa ctttttgcgc tccttggcat cacgtccgat
     3241 cgccagctcg atgagcccat gtcccttctt gggaatcgcc caaaccagcg cttcgtagac
     3301 gcgagtgatg gtatgttgct tgaattggct actaagatgt cggtgtgcct gatcggtctt
     3361 ggcaatgacc atcacgccgg aggtgtcctt atccagtcga tgaacgagac caggccgttc
     3421 ctttccgccg accgcggaca tcgtgccgcc tgaagtctgg aaatagtgaa ggagggcgtt
     3481 gacaagtgtg cctgtccaat ttcctggcgc cggatgcaca acgattccag cgggtttgtt
     3541 gaggaccaga acctgttcgt cctcgaagag gacttcgagc ggaatgggtt caccccgcaa
     3601 ttggagcggt tcgggtttcg ggacgtccat cgtgatctta tcgccgggct tgatcttttg
     3661 actcgctctc acgatctgat cgttgaggcg tatccgtccg agctcgatga ggcgttgcaa
     3721 ggcagaccga gacatgtctc gctcacgatt gacgagaaaa acgtccagcc gcttcggctg
     3781 ttcaccggct gtgaccacaa actcggtaat catgtctgcg gcacacgcta ctaaagtgcc
     3841 atcctcaatt gcaatcagcc tgtttgcctc acgctagccg cagagctatg ctacagagga
     3901 cagccgtctt gaacttgaaa ggagatggaa tcatgagatg ggtcctggcc ttgggcatga
     3961 tgatgctgac tgccggctct ttcttgactg cttcaaaggc ggccgatcag ccgggcattc
     4021 cccctgaaac ggttgccgac tatctgcatt cgatcattga agctgaccga accttctatt
     4081 ccattcacgt cgtcgaacgc ctgcagaaag gtggcgggac aaaagcggct gaagattggc
     4141 gcgcgaagaa gaatgtgctt ccgcttcccg cccaattcct gattgaggcg agcgaactcg
     4201 cagccaagac cggttcgaag gtccgctata gattgatcag cctgtggccg atcaatccga
     4261 agaatgcacc cgacggccca ctggaaaggc aaagtctcga ggccttacgg gagcacccgg
     4321 aacggtcggt gatgggaacc gtcaagcggg gagacaccac ctattttcaa gccatgtatg
     4381 ccgatcgtgc ggtgagcaaa acctgcatcg cctgccacaa tgggcatcga aagagtccca
     4441 aaaaagactt cacgttgcat gaggtcatgg ggggattaat catcgagatc cctttggaat
     4501 gacgcccata gacggcgagg actggcggat ggcatgcacc agcatctgtt gccggcgata
     4561 aattgagagt gcctgctctg tggcttcttc cgcttcctgg tgatgtcgga gtgtctgatg
     4621 aagagatgcc aataacgacc aggctgctgc ttcgccggga agattccgga gcgattgaaa
     4681 cagcgagacc gcacgtcgag cggaatcaac cccttgttcg ggctggcctg cgaggtcatg
     4741 aaccgcggcc aggcgaaatc gcagaccagc ctctatgtgg gcctgaggtc gctgctcgac
     4801 gaaggcaagc gattggccgt aatagctttt cgccggttcg aatcgttcaa gaatgaagtt
     4861 ggcatcgcct agtgcaagca gcgcggagac agtctgctca tcgtcctgac ggctcctcgc
     4921 atcctcgagc gcttgctcga gatcccccag ccccttgtcc gggaatgcat cccgcacttt
     4981 gagaagcccg gataggattt tgacagatcc cagcgcctcg acacgctgaa ggcggcgata
     5041 ttgttccccg gcccggtcca agaagtgacc ggcgtcttcc gacgaaccca cccaaaacga
     5101 cacttttgcc aggaggaaca atgcatctgc atgcgatgca gaatcaggaa attttgaaaa
     5161 tagactcaac gcttcccgaa gccgtagctc agcctcctgt cttcggccct gccgttcgaa
     5221 caccgaaccg atcttcgtca gagcagtcgc ttcgcctctc cgctgcttgc gggcgcgaaa
     5281 tgattggagc gcctgttcat aataagtaag agcttccgga aaatggtttt gcacatcgtg
     5341 aatgtcgcca atgcgaagca gttcgtcgcc gctcaaggct ctcaccggca cgggttcgcc
     5401 gactgtctca aaatttgata tggggctgct cgacgagaga agggcagcca tcacgatgta
     5461 ggagaatccc acgagacgac ctgccgggaa gaggtggaga taaagaatac cggcgtaccg
     5521 attgagcggt caaacgacac attcggcgag aacgcgcgag gcgtgggaca ggattagcgc
     5581 ttgcccgcgc gttcggcgat tttgcggcgg aggcgggctt tttcgaggct gatttcggct
     5641 tctttgacct ccgagggcaa gccgccggcc ttgagacgcc gctcagcttg ttccacggcg
     5701 gcctgggcac gacccacgtc aatatcttcg gccttttcag cgatttcggc catcacggtg
     5761 actttgttcg gcgtgacctc cgcatagccc cagaggatgg ccatatgatg cacctggtcg
     5821 cccagccggt agcggagttc gccaatgcgt aaggtcgaaa ggaaatggca atgacctggc
     5881 aacacgccga attcgccttc cgagccgggc gcaatgactt catccacctg ctggctcagc
     5941 agttgcttct cgggtgtgac cacttccaat aaaatcttcc cggccattgc ctctctcgct
     6001 ggctaaagtt tcacgcccat tttttcggct ttggcaatca cttcttcgat ggggcccacc
     6061 atataaaagg cttgctcggg caaatgatcg tacttcccgt cgaggatttc cctgaagctt
     6121 cgaacggtat ccttcaactt cacgtacttt cctggcgccc cggtaaacgc ttcggcgaca
     6181 tggaaaggct gcgagagaaa ccgctggatc tttcgtgcac gagccacgac catcttgtcg
     6241 tcttcggaca gttcgtccat gccgaggatc gcgataatgt cttgcaggtc cttatagcgc
     6301 tgcaacacag attggacacc gcgtgcgacc ttgtaatgtt cttcgccaag gatttgcgga
     6361 tcgaggatgc gcgaagtgga gtcgagcggg tccaccgccg gataaatacc caattcggcc
     6421 aactgccgcg acaagacggt ggtggcatcc aggtgggcga aggcggtcgc cggagcggga
     6481 tccgtgaggt cgtccgccgg gacataaatc gcctggaccg aggtgattga tccgcgcttc
     6541 gtcgacgtga tccgttcctg caaggcgccc atttccgtcg aaagagtcgg ctgatagccg
     6601 acggccgaag gcatgcgtcc gagcagtgcc gaaacttcag aaccggcttg tgtaaaacgg
     6661 aagatgttat ccacgaagag cagcacgtcc tgattctctt catcacgaaa atactctgcg
     6721 accgtcagcc cggtcaggcc gacacgcaac cgggcacccg gcggctcgtt catctggccg
     6781 tagacgagcg ccgccttaga cttggtgaaa tcgtcgggat cgataacctt cgactcttgc
     6841 atctcgtgcc aaaggtcatt gccttctcga gttcgttcgc ctaccccagc gaacactgag
     6901 aatccgccgt gatgcagcgc gatgttgtta atgagttcca tgataatgac ggttttgccg
     6961 acgccggccc ccccgaagag accgatcttt ccacccttac tgtatggctc gagcagatcg
     7021 acaaccttga ttcccgtctc gaggacttcg gtcttggttt cctgatcttc cagcttcggc
     7081 gcgggccggt gaatcggata ggtttttttg gtcttgatgg gccctttctc atcgacgggc
     7141 tcaccgagca cgttcatcaa acggccgagt gtttcacgac caaccggaac ggagataggc
     7201 gcaccggtgt cctgcacatc catccctcga gttaagccgt ccgtcgagga catcgcgacg
     7261 cctcgaacac ggttttcacc caggtgctgg gccacttcaa gggtcaaccg gacggctggc
     7321 ttgccggccg ccatgttctc ttcctgattg actttgatcg cattgtagat gttcggcagc
     7381 tggccgggcg ggaattccac atccacgacc ggaccgatga cttgaatgac tttgcctgta
     7441 ctcacgttgt ccccctttgc tcgcatgctg aggagcctgg tcagggtcct tcagcaggga
     7501 accctcagca cttccagcag ctcctatttc aacgcttcgg ctccgccgac gatatccatc
     7561 aattccttgg tgatcgccgc ttgtcccgtc ttgttgtaag acagcgtgac tttctgaatc
     7621 agctgaccta cgactttgcg ccgtacccca ttttctgttt gaacgtcgtg atgatctctt
     7681 tgagcttcgc cgtcagtttc tcgtcgattt taccggcgcc ggcgacttcc tttttcagat
     7741 ctggatggtg ttgctgcgca tagtggagca ggtctgcctc aaactgctgc accttatcca
     7801 ccggaacgtc atccaagaac ccgttcacgc ccgcatagat tgagatcact tgatcggcaa
     7861 ccggcatcgg tttgtactgg ccttgcttca acaattcgac catgcgaacg ccgcgggcaa
     7921 gttgcatttg cgtcgcctta tccagctcgc tgccgaattg ggcaaaagcg gccatttcac
     7981 gatactgggc caggtccaag cggagagtgc cggcgacttg cttcatcgtc ttgatttgag
     8041 cggaaccacc gacacgcgac accgagaggc cgacgttgat cgcgggacga ataccggaat
     8101 aaaaaagatc gctgcccaga tagatctgcc cgtctgtgat ggagatgaca ttcgtcggga
     8161 tataggccga gacgtcaccg gcctgcgttt cgatgatggg cagcgccgta agacttcccc
     8221 cacccaattt atcgctcagt ttcgccgccc gctcgagcag ccttgagtgc agataaaaga
     8281 cgtcacccgg ataggcttcg cgcccgggcg gccttcgtag gagcagcgaa agctgccggt
     8341 aggccaccgc gtgcttggag agatcatcgt agacgatcaa ggcatgctta ccgtggtccc
     8401 ggaaatactc accgatcgcg gcaccggcga atggcgccag gaattgcatg ggagccgcat
     8461 cgctggcggt agccgagacc accatgctgt agtccattgc atgatgttct tcgagggttt
     8521 ttaccacgcg ggccaccgta gagcgtttct gaccgacggc cacgtagatg caaaaaacac
     8581 cgaggcctct ctggttgatg atcgtatcga cggcgatcgc cgtcttgccc gtctgacgat
     8641 ctccaatgat caactcgcgc tggcctcgcc cgatgggaat catggcatcg atggccttga
     8701 tgccggtctg taatggctcg cgcaccgatt gcctggtgtt cacacccggc gccacgacct
     8761 cgatccgtga tgaatgctgt gacttgatcg gccctttgcc gtcgatcggc tggccgatcg
     8821 cattgaccac tcggccgatc agggcgtcgc cgacggggat ttccgcaatg cggcccgtgc
     8881 gcttgaccgg gtcgccttct ttgattccga catcgtcgcc catcaacacg gcaccgacgt
     8941 tgtcttcttc caggttcaag gcaatgccat acagaccgcc gggaaattca agcatttcac
     9001 ctgccatggc gccatcgagt ccgtagacct tcgcaatgcc gtcgccgacc tgtatcacag
     9061 agccggtctc tttgacgtcg acctgcttgt cgaacccctt gatcttttcc ttgatgatcg
     9121 cactgatttc atctgccttg atctgcatgg ctactccttg ctcgtcgcaa cggtcacaga
     9181 tttcctcgtc ttcagctccg gaacgctcgt cacgcgaaag cggcaggttt gcctgtcttt
     9241 cgagggaggc gtgtcgtcct tttcagtctc gggtcagcaa cgactgcatg gcattcaagc
     9301 gtcctcgaac ggtgctgtcg acgacggtgc tgccgatatg aattcgcagg cccgcaaggt
     9361 gcgtcgcatc agtgtgaaac gtgacctcga cctcccgctt cagcgtctcg cgcaaccgcc
     9421 ccttgatgcg gtcctgttcg gctgggggca acgccgcagc cgaagatacc gtgacttgct
     9481 gcgttccttt cgactgatcg accagtttgg caaacgcctc cgcaatatcg gggaggaacc
     9541 cgactcggtt cttcttgacc agttgtccaa agaaggcttt gccggccggc ggacaaccga
     9601 gacgctcgcc aagctctgtg aggacgacgt tcctctcctc gacaccgaat gcaggagagg
     9661 aaacgacatg ccggagagca gacgactctt tcatggcctg gcctagacca tgcagggtac
     9721 gccgcatcgc ttcgatgttc gattgatcga gaagctcgaa gagggcctgg gcgtaacgtc
     9781 gcgcaactgc tgtcttgatc actgtcctga tccgctcttt ggtatttaag gcaaacggga
     9841 tgcttccgtg caggaggcac gaaaataggc gcgcaaacct agcattggcg gatgggaagt
     9901 gtcaatacag acgggccgag acgaaggcgg tcagatggcc tgaatcatcc cgccgccgag
     9961 cacccggtca ccgtggtaca gcacggcgga ttgcccgggg ctcagggctc gctgcggcac
    10021 atgaaaggcg aggtgcaacc tgtcctggtc gatcggttca gccgtcgctg cggttgccgg
    10081 cgtcgcatac cgaatcttca cggcaacatc cgtcttgccg gtgcacaaac tacgatcaaa
    10141 cagattgagc ccctgtatat gacatgccga actgagtaag gtctcttctg gacccaggac
    10201 gacagtatta gtcaccggca tgacctgccg cacgtagaga cgctgaccgg ccgcaatgcc
    10261 gagcccgcgt cgctgtcccg gcgtataaaa ggcaatcccg tcgtggcgtc ccagatagcg
    10321 cccattgcca tcgacgaaat cgccgggacg ccttgtgtcc ggcatttctg attcgatgaa
    10381 ggtgcgataa tcaccatggc tcacaaagca aatctcctgg ctttccttta attcatcgac
    10441 tggaagccca agggcctccg cctccgccca tacatcggcc ttctgcatgc cgccaacggg
    10501 aaatcgcaga cggggaagcc aagcgggatt gagacgatag aggaaataac tctggtcctt
    10561 tttttcgtcc agggcacggt ggagcgcata ctgaccgccg cattcctgaa cccgaacgta
    10621 atgtcccgtt gcgacgtatt ggattccgcg ctgttcggcc aggtcgatga gacctcggag
    10681 cttcacccgt tcgttgcaac ggacgcaagg attcggcgtg atccctccgg catagccgtc
    10741 aaggaaatcc ttgatgacac tctcgcgaaa gttctgccgc tcatcgacga cctcatacga
    10801 aatgttgagg cgctgtgcga catacctggc gatgccgatc ttgcaacaac cgcgctcctg
    10861 ccatttcttg gacgccgcga cggaatcgtc ttcgtgttcc cagacttgaa gggtgacgcc
    10921 atgcacctca tagccgtcac gtaccaggag agctgctgcc acagaactgt cgacgccgcc
    10981 gctcattccg agcaacaccg tgggcttctt cattgtgacg gtattgtacc ggcagaagag
    11041 gagaaatggc cagtggctgg caaacggaca ggcgaagcgc gccggactta ggaggcgatc
    11101 gagaccgcgg cgggctcaga ccacgattgc acacccatgt cgcacatgcc gtggaaatgc
    11161 gacccttcct caatcgcaat caacggcgtt cgaatatccc cgaccaggac gcccggcttc
    11221 agaatatgaa ttttttccac ggctgtaatt gtgccgttga tctttccact gttcatcagg
    11281 acgcccgccg acacaatgcc cttgatcatg gcatgctcgc ccacgatcag cgtgccggtg
    11341 gtatgaattt ctccttccac tcggccatcg acccggaccg ttccgtcaaa attgaccaca
    11401 cctttaaagt cgacatcttt cgccagtagc gtaaagtttt caaaatcggg ttctatgtgt
    11461 tcgacttgat cttttcttgc ccacatgatt cctccgctta ttattggtat attagcttag
    11521 gtaactttcc gatcaactca cgcgcttcac tgcggcaggc ggagtgccga tctggtgtaa
    11581 tgcggttccg tcacgcatcc cttggctcat ctcgaggccg ccgttgaaca ggacgccgtc
    11641 ttcgatcgac aacatcggcg ttttcatgct cccattgaac acggcaggag ctcgcagctt
    11701 gacccgttct tttgcagcga tatctcccgt gatctttccc ttgcagacga tcgtccccgc
    11761 ggtaaccttg gcttgaatca ccgcctcatc tcccaccagc aacacgccgt ccgtatggat
    11821 ttcgccatcg aggaagccat cgatcctgac ggtaccgctg taggagatga cgcccttgaa
    11881 ctcgacgcct ttgccgacaa aggcaatgat atcctccgtc ccctcacttg ctcggctgac
    11941 cggctcggtg ccctcttcgt tctcaccttc gacggcacgc ttgccttctt gtttatcctg
    12001 gccccacatg agtcgtcctc cccagaatgt ctcgccgact tttctcgttg tggaccgctg
    12061 tgagcgagca gcatcgatcg cacgacaaac actgccactg gtcaggcgat taagcaacaa
    12121 atatgcagat tacttccgaa gggtcgtgac gaggtacgta gagggagtta ctgaagcagc
    12181 tgctccagaa gaccgtcgag ttcggctgaa gagaaatagt gaatgatcac ttttcctccg
    12241 cgtcttccat taagaacgga aacttttgtt ccaagccgtt tttgcaaacg ttcttcaaca
    12301 tcagagaacg gtgagcggcg tagctctttt gcatggcgtt tcctggcgat gaggccggat
    12361 gaaagaatgt gttctgtctc acggactgag agatgacggc tgacggcgag atgcgccacg
    12421 cggagttgtt ccgcagggct cgcgagcccc aagatgactt tcgcatgacc ggcagacaat
    12481 tgatcggtct caatcaggtg ttggacttcg accgggagtt ggagaagacg aacagcattc
    12541 gcgaccgatg agcgctcgca gccgacgcgc tgcgcaacaa tgtcctgcgt gagtccaaat
    12601 tcgttcatca tccgttgata cgctcgagcc atttcgatcg gattgaggtc ttctcgttgc
    12661 agattttcga ccagcgccaa gaggatggat tcttcatcac tacagtttcg tatcattgct
    12721 tgaatggtct caagcccggc ctctctggcc gccctccaac gccgctcgcc cgaaatcaat
    12781 tcatacatgc catcgccttt tcgccggaca agcacgggct gaagtaatcc gctttgcttg
    12841 agtgacgccg caagctcagc cagttcttga ggaggaaagg tttggcgcgg ttggtagcga
    12901 ttcggcacga tggcgtcgat tcgaagatgc tgaacttccg gcaattccgg catgggagca
    12961 ggttttgtcg tcggcaacaa cgcgtcgagt cccctaccga gggccttttt ctccatgatt
    13021 gataaactcc ttcgctagag attggtaggc ctgtgcaccg gctgaggcca tgttatagag
    13081 cagaaccgga cggccgtagc ttggagcctc tgcaagcgtc acgttccgtg ggatggtcgt
    13141 ctgatacacg aggtctttga agtgtccacg tacttgctcg gcaacttggc gcgccaacgt
    13201 attcctcggg tcgtacattg tcaagacgat gccctcgatg gtgagcccgg gattccagga
    13261 ctgctgaatc cgttcgatac tgccgatcaa tcggctgagg ccttccatcg cataatattc
    13321 gcattgaacg ggaatgagca ccgagcgagc agccacaaga gcgttaacgg tcaatatccc
    13381 caatgcaggt gggcaatcaa ggaaaatgat gtcaaacaga gactctacgc cgctaaggaa
    13441 gctgcgtaat agcttctcac gctcctccac agtaacgagt tcgatttctg ctcccgccaa
    13501 gtcagagttg gcagggagaa gtaataagcc gctgacttcg gttttgcgaa cggtttcatc
    13561 aaattttacg taatttatca aacagttata gacagttttg tcgagtgatc tcggatcaat
    13621 ccccagcccg ctggtcgcat tgccctgggg gtccatatca acaagcagag tacgtttgcc
    13681 ctgtaaggca agtcctgctg ccaaattgac agatgtcgtc gttttcccga cgccgccctt
    13741 ttggttggct acagcaacaa tcagtgccat cagatccaat ctagggaaac atgttccacg
    13801 tggaacatca cgccgctacc gcctgggaaa gtatcgtcag gacacgagtt ccatagttcc
    13861 tcgggagtga gaatggatat tgggacttaa tggtgaacga gctggatgtc gcgagcccag
    13921 cctcactatc ccctctcccg gtgccataga gcaccacttt cccaattggc ttcagaacgc
    13981 tgggcgcaac aggtgccacc tggtcaaacc gaagcgctcg aacaaccatc aaatcgccga
    14041 gagtgtgatg ctcctcaacc gcatattccc caatcgtgcc cgagaatatg cagacattct
    14101 taagctggag ggtaccgaca acagcccgaa ggaatgacga cttcttttgg gtaggttcta
    14161 tcaagacaag ccggagatcc tgccgcacga tttttaaagg tattccaggg aagcctccac
    14221 cgcttcccac gtcaatcacg acaccttcga ttggaaaatc gacagccacc aaggcggcaa
    14281 gagaatccac aaagtgcttg ccgatgatct catacggatc ggttatgctg gtcagattaa
    14341 tcgtgctgtt ccacctgagt agctgcgcga gataggtcca gaattgctcg atctgcgtat
    14401 ttgtgagaga gagaccaagc gtttgcgcag cggcttgaag gaactggcgc aactcacgct
    14461 cttgttccac gtggaacaaa tacgtgaaac acaaaataac gtcaagcttc ttgttgggga
    14521 tgctttctaa ggaatggcct gagcggctga ctgctctttg tatttttcaa gggccacgat
    14581 tagcagtgag atggctgcgg gagttacgcc agagatcctg gaggcttgac caatggaggc
    14641 aggacgcacg cgttggagct tttcccgaac ctcttgagaa aaaccacgaa tggcatcata
    14701 cgggaaggca ttgggaatcg ctctcgtttc cagcttcttg aagcgtccga tttgttgcat
    14761 ctgtcgcttg atatagccct gatattttat ctgaatttcg atttcctcga taatttcagg
    14821 attgtggtgc cgatcaagtc cgagacaatc taacaaagtt tcataatcaa ctccttggcg
    14881 ccggagtaac tgagcgagcg aacaaggtcc tccaacttct ccaagcttag cgtccattaa
    14941 cctttgcctg atgtcatcgg tgacatggag ccttgatccc tcgagcctac tgacttcgtc
    15001 gtcgatctgt ttctgtttta cgcaaaactt tgaatagata tcgtctgaga tcaatccgac
    15061 ctgtcggccg atttcggtca atcgcagatc ggcattgcca tgtcgcaata gaagtcgata
    15121 ttcggcacgc gacgtaaaca tgcggtaggg ttcatgggca tctttagtga tgagatcgtc
    15181 gatgaggact ccaatgtagg cctcggatct gtcgaggacc aacggcgggc gttctcgcag
    15241 cttgaggact gcgttcactc cggccatgag gccttgggct gcagcctcct catatccgga
    15301 cgtgccgttg atttgcccag catgataaag cccttcaaca agcttggttt ccagagtcgg
    15361 atgcaactgt cgaggaggaa aatagtcgta ttcgattgca tagccaggct tgagcatgcg
    15421 ggcttgctca agaccgggta ttgttttgag catggccatt tgcacatcaa ccggtaggct
    15481 cgtcgagata ccgttgggat agaattccgg agtatcgagg ccctccggct caacgaaaat
    15541 ttgatgccgt gtcttttcgg caaatcgaac gaccttatcc tcaatcgatg gacaataccg
    15601 aggtcccacc gattcgatga cgccgctata caacggagat cgatcgagat tgccccggat
    15661 aatgtcatgc gtgaccgcgt tggtataggt caggtaacaa tccatctggc gaacgcgaat
    15721 ccggtcagtt cgataagaaa agggcggcgg cggatcatcg ccgggttgcg gaatcatggc
    15781 agaaaaatca atggtgtcac ggtcgagccg aggtggggtg ccggttttca accgcccgat
    15841 ttcgaaaccg agatcccgca tacaatcgga aaggtgttca gccgacactt cgcctgcgcg
    15901 gccagccgga aaatgattca gtccgatgtg gattaaaccc ttaaggaatg tacctgatgt
    15961 tatgatgacg gcgcgggcgc ccagacgctc gccgcagtcg gtcaccacac cgcagatcgt
    16021 gtgtcccttc agcagcagcc gatccaccgt gccttgcccg atggtgagcc cgggctgccc
    16081 ctccaatgac cggcgcattg cctcgcgata catcttctta tcgcactgtg cgcgaagagc
    16141 gcggaccgcc ggccctttgc tggtattaat gaaccggaat tgaattccgg cgcgatcggt
    16201 gttgatggcc atttccccac cgagggcatc gatctccttc acgagatgtc ctttggcaat
    16261 gcctccgatc gccggattac aggacatttg agcgacccgg tctctctcca tggtcaacag
    16321 gagggtctgt gcgcccatgc gagcggcggc gagagcggct tcgcagcctg catgcccgcc
    16381 tcccacgacg ataacgtcaa acgaacgtga catcacgcac ctttctctca tttcccgatg
    16441 cagaacgtcg aaaaaatccg cgataaaatt tcctcattcg tgatggcacc ggtgatctca
    16501 ccgagggcgt ccgccgctgc gcgcaggtcg acggccacca gttcggcggc catctcgtcc
    16561 ttgatcgagg acgtcgcctc aaccaatgac tcatgggccc gtgtcaaggc ggtctggtga
    16621 cgaacgttcg tgaccagcac gccatcggcg gtctcgggcc cgcttccagt caattgcgct
    16681 cgaatcgcca gtcgcaagtc ctcgaccccg tctcccgttt ttgccgagac cgcgatgcac
    16741 acgctgcccg gatttacaga cgtcggactg acctgggccg ggaggtcggc cttgttgatc
    16801 acgatcacgt gcctcatctc agcggcctga tccatcaagc ggcgatcgtc ggcggtcaat
    16861 ggcaggctgc catcaagcac gatgacgcgc aaatcggcct gctcctgagc cgaccatgag
    16921 cgcttgatcc cttcatgctc caccggatct tccgtgtcac gtatccctgc cgtatcgacc
    16981 aaatgaacca agaccccgcc gaggtcgatg gcttcctcaa tcaggtctct cgtcgtgccg
    17041 ggaaccgctg tgacgatcgc ccgttcttcc ttcagcaagc gattcagcag gctggacttt
    17101 cccacgttgg gacgtccggc aatcacgacg cgtgccccgt cgcgaaggag ccggcccgcc
    17161 cgtgcggtgt ctctaagccg tcctaccagc ctggccacgc gatcaacggt cccgactaga
    17221 tcttgtttgt gaatgatgtc cacgtcttca tcaccaaaat caataccggc ttcgagctga
    17281 gccagcaggt gcactaacgc ttctcttgcc gtgtccacct ccctggccaa atcgccccgc
    17341 aactgtcgct gcgccgcagt gagactggca gcggatttcg ctcgaatcgt atcgagcacc
    17401 gcttcagcct gcgataaatc gagccgaccg ttgagaaagg ctcgcttggt aaactctccg
    17461 ggctccgcca gacgcgcgcc ggcctcgaga caggcgcggc aggtcagaga caggataagt
    17521 gaaccgccat gagattggat ctcgacgata tcctcaccgg tgaacgatcg aggggctttc
    17581 atgtatacga ccaatgcctc gtcgaaacac tctcgcggct tcggtatgga cggatcctgt
    17641 gcgaccgtct gattcatgtc gggaaagcgg aggtcggcaa ggcagagggt gtgggacggg
    17701 acggatgcca acggctgtcc ggaccgcaac tgcacgaact tgtcggcaat ccgcagcgct
    17761 tgtgagccac tgagccgcac aatcccgatg ccgccttcac cggccggagt ggcgatcgcg
    17821 cagattgtat cctcgggtcc tccgcgcaac atggatcctc tcgctcattt tgacggatcg
    17881 gtgacgagcg gcctgatggc aaagaggcgg tcggtcaaca cctgctgtcc gatggtcagc
    17941 acgttgttgg ttagccagta caacaccagt cccgccggaa aattgacgaa taaaaaggtc
    18001 ataaacacgg ggaggaccaa catgattttt gcctgagtcg gatccatcgt cgtcggtgta
    18061 attttctgct gaatcaccat cgtggcgccc atgatgatcg gcaacacgta ataggggtcc
    18121 tgcacggaca gatcagtaat ccacccgaca aaaggggcct gccggagatc gatggtcata
    18181 tagagaatgt tgaagagcgc aacgaagaca ggcatctgca gcaccatcgg cagacagccg
    18241 cccacgggat tgaccttgtg atcacgatac agcttgatca gctccttatt caatttgtcc
    18301 cggtcatcct tatatttttc ctgcagcgcc agcacctttg gctggaccag ttgcatctgc
    18361 ttcatcgact tatagctctt gtactgtagc ggcacgaaca gcattttaat gcccatggtc
    18421 aacagaatga tcgtgacacc atagttatgc gtgtattcgt tgatgaaacg cagcacatag
    18481 aaaatgggtt tcgccactgc cttgacgacg ctccaactgc caaaaatgaa ccagccgaag
    18541 tcgatggtct cttccaagcc cacgtttaac gatcgcagcg tgtcatattc cttgggacca
    18601 gcgtacaatt gcaggtcggt ggtacgtgcg ccggaagccg gcaatcgcac gccggcagaa
    18661 acgagtttgt cgccttcttt cttcgccaag gccgcgctgg caccctgcgg catcaagacc
    18721 gagagaaaat acttgtcttg gagggcaacc cattgcaccg atcccttgcg ttcgagttcg
    18781 agatccggtg tttctttttc aacttttccg tctaccagtg aagccgaacc aatcagacca
    18841 atgaaaccgt ctccccattc gacgatccca aagtttgtgc ccaggacaat gtcgtacgca
    18901 ccgctcaagc catcgaccgt caagctgaca tcgaccaggt aattttcctg acgaaacgtc
    18961 aggcgctttt gcaccttgag gcccgatgca gcgtcgtgaa accgcagggt cacatggccg
    19021 gtcggatggg cgtcatcgag cgtggtgaaa tcggtttcaa tcgcatacag accttcgcgc
    19081 aatgccttgt ctgccgcgac gtccgacgtc gtgatcgaga gcggctcctt gaatttggcc
    19141 ccttggtaca ccagttggac cgggttcccg tcggtagccg acgtgtaatg ccgcttgagc
    19201 tcccaactgg aaatcacccc accacgtgca ttgaaggcca cccggatgaa atcggtttcc
    19261 acggtgacgg tgcgctccgt gggaggaaga gagggacgtg aatcaccgga tgaggcggta
    19321 tccgaagcgg atgaggaggc acggggagcg ctcgccgtcg tctcggcgga aggcgtctca
    19381 tgttgaccgc gaggagccgc agcctcctgg ccgcctggct cttgcacggt cggagtcgcc
    19441 gggagcagcc cgaattgttt caggaggagg tcatagccca ggatgaccgc caacgaaagc
    19501 acaaggaaaa tgacgacgcg tttttccatc tcactccgcc gtcccgcgag tggtcacttc
    19561 gggcatcagc ggatcgtgcc ccccggagtg aaacggatga cacttaagca ggcgccaaac
    19621 cgcaaggccc gaccctcgga tcaccccata gcgctcgatt gcctggccgg catagtcgga
    19681 acacgtgggg tcaaaacggc aggcaggccc gagccacgga gagatccaga atcggtatcc
    19741 attgaggagg gtcacacaca tcgttttcat gctatacctc acggccgctg agcaaacctt
    19801 gacgccgcag cgtagtctgc cacaggcctt tcagttcttg gaacggaagt gtaagggcgt
    19861 ctcgctttgg aaatacgagc aaggcatgat tggaggtcag ctcgtttcga acctgtctcg
    19921 tcagctcccg gaacacacgc ttgacccgat tgcgacgaac ggcaatgccg aaccgtcgcc
    19981 ccacaatgat gccgacgcgg ctcgtgccgc cttcggtctt gcacaccagc aggttgaagc
    20041 aggctgtgga cagccgccgg ccatgctgtt tcacaaactc aatgtcgcgg ctcgcgcgaa
    20101 gaaaaaggat tcgatctcct gccctccgtg acatgatcag gatgattcgt aggggcttgt
    20161 ccggacccct ccgctcgact ctgcgtcccg agctcggccg gaccgctcac cgtcaggttc
    20221 ttagaaagac gtccctgctt gaggaagaca agggggatga actccgtgcg gcgtcgaaaa
    20281 gcagtgctag accgtcaaac gcgcgcggcc cttggctcgg cggcgggcca agaccttccg
    20341 gccgtttttt gtgctcatgc gttttcggaa tccgtgttcg cgcttctttt tgaggttcga
    20401 tggctgttga aacgtaaacg acatacagat cgcctttccg acagatacgg aattgaaggt
    20461 cgcattatag ggacgagcct ccaccctgtc aaacctcgcg acctctcgac ttcttgccaa
    20521 tgattcaaga caatcgtgcg tgcaaggata tcagctaagt ctttgataac ccgttcagat
    20581 tgaggcggtt ttgtggacct ttttcccaaa atgaactaga ctgccacaca gtgtgttccc
    20641 acgaatgagc atgcatgctc taacgtgttg ctgaatcaag gcaagaacga gtgtccagtc
    20701 tttctcgctc tggcactacc tttgcgaaat cgatagggaa gacaagcagt ctacgggtgt
    20761 gggcacacca agtgtggacg cgacttgcgt ccgtaatcag gaggactacc gatatgaaca
    20821 tgcgccatgt cttggtcgca gtcctgagtg catgggtgtt ggccgccggt tgtgcacagc
    20881 cgccagccga ccagatcaac gccgcagaac aggcggtcaa agatgcccag caaagcggtg
    20941 ccgcgacgta tgccgccaac gactatgcca aggtcgaagg attgctgacg gccatgaagc
    21001 aggaagttgc ggatcaagac gcaaaatttg ccttgcttcg agactatgga aaggccgaac
    21061 agctcgccgc aacaaccaag gccgaggctg aacgggtcaa ggcggatgcc accaagaaaa
    21121 tggaagaagc gaagtttgcg gccacccaag cccaacatgg agccgaggcc gcagtccaat
    21181 cgacgcttga gctcgtttcc agggctcccg ccggtaaaga ccgcgcggca cttgaatcga
    21241 tcaaaacgga tgcacaagcg ctcaaggctt cactcaacga cgtccagttg atgctcgaca
    21301 aatcggatta tctcggagcg cacaccaaag ccaaggcgat tcgggagaag gctgaagccg
    21361 tctcacatga agttcaaacg gcgttggcca agatcggcaa agggacggcc agcaagaaga
    21421 agtaaccacc tatgcgtgag atgccacttc gacatgcagt gcggatcgtg tgctattcaa
    21481 gcatgttcgc gctgtgcggg tcgcttgccg catgcgtaga aagcgtgccc tccgacgtcg
    21541 tgcaggccat tgaatcggtc gataacgatt tggcgcagct ccgggcggct gagctggcgc
    21601 ccagtgcgta caacgccttt gcgtatcagt gggtcacctt gcgtagtcgt gtcgaggccg
    21661 aagaagatac cgttcgctgg ccttgggaat cgaacgactt ggaagcagcg ctccgcggcc
    21721 tgcacgcgga ggggacgaga accgttgcac tcctgacgga acagcaggaa acgctgcggc
    21781 gctcagccgc acgccaactt gctgaagtgg aggcacaggc ccatgtgctg accactcacg
    21841 tgggagcgat tgacagccga ctgattctcg gagaaaaacc gatcgaaacc gacttgctgc
    21901 tgaagcaggc ccgtacattt tacgaacaac ggcgatacga acagtcattg agcgcctctc
    21961 agcgagcatt ccactacctc gcggctcagt ccgcgttgct gcaacgagaa ttagggcggt
    22021 atgccagtcc agatcgcgtc gcccgctggc agcgcatggc caaggagacg atcgaatggt
    22081 cgcggaccca caagagggcc gccatcgtcg tcagcaaagc ggagcgatcc ttaacgctct
    22141 atcgcaacgg acaaaaagtg gtatcctacc ccgtgcgact gggctttaac ggcatcaagg
    22201 aaaagcgata tcaaggcgat ggcgcaacac ctgaaggccg atatcgcatc ttgagcaagc
    22261 gaggacgggg gcagacccaa ttctatcgtg ccttgttact ggactatccc aaccaggacg
    22321 atcggcggcg ctttctctcc gagcgcaaaa tggggaacat tcccatgttg cgcgggattg
    22381 gagggcaaat cgagatccat ggcgtggaaa acgagttgat ggctcaaacg ttggggtgcg
    22441 tcatgctcga gaacgtccag atggccgccc tcttcgatcg tgtagacgcc ggtacgccgg
    22501 tgaccatagt cggagcgctt ctcgaacgga attcagtcgc aaaggccctg gcgagtcttt
    22561 ctgggcagcg aaacgaaatc tgacgggaca tgccacgcat tgtagcgtct ctctgcttgg
    22621 gactcatcgg cttagtcctg ttgacgttgg cacagagcag cagtctcacc gtcaccgatg
    22681 cttccattcc agccgtcata cccgacccgg caacgttaca cgccttgcaa acccgctacc
    22741 gatccctgtc caaacaactc aaccatttga tgcccagcca gccatatatc gtcgtggata
    22801 cggcgagaaa ccatctgtat atcaaacggc gggaccacgt gatgttggat gcgctcgcct
    22861 caaccggcag cggcaccatt ctggacaagc ccggagaagc caaaggccag tgggtgtttg
    22921 ataccccacg gggggaattc accgttcaat cgaagctcat caatccggtg tgggtgaagc
    22981 cggattgggc cttcgttgaa gaaggattgg ccatcccgaa gagttacacg gaccgcatcg
    23041 agccgggcgt actgggggaa tatgcactgg ggttcggcaa aggcttcttc attcatggca
    23101 ctctctatac gagactcctg ggaaaaaatg tcacgcacgg ttgcattcga ctgaacgatg
    23161 acgatctgaa gagcgtgtat cgcctggcaa aggttggcac gcccatcatg attttctagc
    23221 tgaatgcctc gcccatccgt caccgggatc ctcatttgct tcttcgtgct aacggccgct
    23281 ccgcctactc gagcggtaga tgctgactat cccctgtcag tcgacgtgac gaatccggct
    23341 gcgctgcaag aagccacccg cattcttgcc gaagagttga agctggcgtc tcgtccacag
    23401 gcgtacgtca ttgtggatct catcgatcga gccgtgctca tcaagggccg cggtgtcgag
    23461 ttgcaccgct tctcgatcga acactggtcg gccggccaac tcgccgacgc ttcgactacg
    23521 ttccgtctgc agacgcggcc accggtgacc agacgcaaaa tcgagcctgc ggccggcggt
    23581 gaacaaccac ccatctccct ggatgacatg ccaactgatt ttacgctgca gttttccccg
    23641 cccttctcac tcaccgttca cccttcggca tccgacgata tcgggagatg gcttcgatct
    23701 aaaggacgtg aatggtggat gtggctcaaa gtctgggcca tgacgttggc gaccggtcga
    23761 gagcctcttc ctcagccatg gctgcatttg accgtgacat ctgatcatgc gcaatcgctg
    23821 gcttggacgg tcacggaggg catggccttc ctcattcgcc gtccccctcc gtcgccctag
    23881 gccaccgttt gccttttctc caacaccgca cctaagatgc ccatatggtg gagtcgtgcc
    23941 tgcctggcct cagtcaatca cgtacacgtg ctgcaacgct cttcaatttc ttggacgcct
    24001 cgtgcgaccg gcaccctaca gtgcggcctc gtgtcgaaga gggccggcgg ccaggggtcg
    24061 caaccggtcc tcgtccgtcg cccacggaat aactagagcc atgtcacccc ctgctgaaat
    24121 ccgcatcgct caggactcat tcacatgggc gacggaagcc gcgaccctga cccaggaatt
    24181 gcgccggcag gcggtgaaag agcgggggct gtttcttctt gcgctgtctg gaggccggac
    24241 gccggagcaa ctgtatcgac gattggcttc ctcgcccttt tgtgatcaag gagattggac
    24301 caacacggag tttttcttca gcgacgaacg ttgtgtccct gcccatcacc ccgacagcaa
    24361 tttcaatctt gccgacgaaa ccctttttcg ccctctggac atcccttcac atcgcattca
    24421 tcgcatggcc tgcgaacagg ccgatgccga ccttgcggct cacgactacg aacaagtatt
    24481 gcgtactgtc accaagacgc ggccagaagg gtggccccgg ttggatctgg tcttgctggg
    24541 catcggacaa gatggtcaca ctgcctcctt gttccccggc accgatgccg tgcaagaacg
    24601 acggcgttgc gtgacggtgg gatacgcccc ttccgggcct cggaccagac tcaccctcac
    24661 cctacctgtg atcaatcaag cgactgtggt actgttcctc gtcactggta cggcaaaggc
    24721 cgaaatcgtg aaaaccattt tggaacccca gcaggaagtc gaccggctgt tgcctgccgc
    24781 tctcgtccga ccggaaggag gccgcctcat ctggttgctg gatccgcccg ccgccgctca
    24841 gctgaccggg catctcgaaa gcaattcacc gaggtaccta tgatccttgc cggggatatc
    24901 ggtgggacga agacgcatct cgcgctgttt gattggacga ccgagcgggt cgaccctctg
    24961 cggctcgaaa cattccatag cgccgactat gcctccctcg aagacatgct cgaggaattt
    25021 cttgcgccgc ccaaaccgcc gacgcccatc gatatccttg agtcgaggcc gcaggacgag
    25081 ccgtcatctc ccccgcagga cgaactgcca ccgattcgcg cggcttgctt tggggtggct
    25141 ggaccggtca ttcaaaatca tagtcgaacc accaacttgc cctgggtggt tgatggtgcg
    25201 gcgctggcca agcggttcga attacccgcc gtacagctgc tcaacgacct cgaggcgatg
    25261 gcgcacggca ttgtgctcct ccatgccgat gaggtggtca ccctgaacca cggcacgccg
    25321 ccagtgcaaa agcaggcaat tgcgctcatt gcggcgggga ccggtctggg tgaatccatc
    25381 ctgttctggg acgggacgcg ctataaaccc ctcccgtcgg aaggcggtca cgcggatttt
    25441 gctccaaaca gcgatcatga gatcgaactc ctccgataca tccgcagcca gtatctgcat
    25501 gtcagttatg agcgggtgct ctcgggaccc ggcctgcatg ccatctatga atttctccgc
    25561 gatgcgaagc gaaacgagcc gacctggctg gctgagaaaa tcaaagccgg cgaccccgcg
    25621 gccgaaatcg ccgaggcggg attgaagggc caagctgaaa ttgccaaaca agccttggac
    25681 cttttcgcct ctatctatgg cgcagaagcg gggaatctgg cgctcaaagc catggccatc
    25741 aatggtgtct atgtgggagg cggcatcgct ccgaagcttt tggccaaact ccaagacggc
    25801 acattcatga aagcctttac caacaaaggt cgttacaaac ggctgatggg tacaatcccc
    25861 gtacatgtcg tcatgaatca gcataccggc cttctcggcg ccgcatcgac ggcggcccgg
    25921 ttggcgcaag catgagcacg tctgacctcg acaccctcaa acatgccgcc gccgcgaagg
    25981 ccgttgagtt cgtgaaagac ggcatggttc tgggattggg caccggttcg actgccaagc
    26041 atctgatcat ggccttgggc gaaaaagtga aggccggtat gaacctcagc ggcgtctcaa
    26101 cgtcacagga gactgcgatg ctcgcccgac agtgcgggat cgccctgatt gacgcggaag
    26161 cctgctggac gatcgatgtc gcctttgacg gcgccgacca agttgatccc gcgttcaact
    26221 tgatcaaagg tggtggcggg gcgctcttga aggagaagat tgtcgcggca tccgccatga
    26281 agtttgtcgt gatggtagat tttaccaagc gggtcacggt gttgggaggc gcctttccgc
    26341 tgcctgtgga agtcgtcccc ttcgggtggc gcaacacggc acggaaaatt gaggagctca
    26401 cccgaagccg cgtcgtcctt agggaacgag agggcgcacg cttccagacc gagtcgggca
    26461 acctgattgt ggatgtccat ctggcacgga tcgaccgacc cgatgagttg gaaacagcgc
    26521 tccatcggat tcccggtgtg gttgaggccg gtctctttgt cggccgaacc gacgtcctga
    26581 ttgtcggaac cccagatcga acggagatcc atcacgtcaa gcggggatga aaggacgttc
    26641 acgagacgtg acacgctgct gcgggttgtc aggagtacgg gcttcctcct ggccgttccg
    26701 atgatcgcgt gcctcccccg aatccccccg gccacagtcg actcggcatt acctgcagcg
    26761 aaagggaaag atatgaacga ggtccctcac ggtcttgacg atccttatcg gcttccgcag
    26821 catgtgcgtc caacacgcta tcacctcata ctcgaaccgg atcttgtcag cgccaccttt
    26881 caaggacgtg cgaccatcgc attgacggtg ctgaaggaga cgcgtgtcat tgtctgcaac
    26941 gcgctcgaac tggccatcgg cgaggccgtc gtcgaggcag gagggcgacg acagaccgcc
    27001 tcagtcacgc tcgacgaatc gttgcaacgc tgtcgaatga cagtcccaga gccactcgcc
    27061 ccaggagcgt ggcagctgca tgtcggcttt cggggcatac tcaacgataa acttcgcggc
    27121 ttttatcgaa gtacctacac agatcaacgc gggagccaac agacgatcgc agcaacacaa
    27181 ttcgaagcca cagacgcgcg acgcgccttc ccgtgttggg acgaacccag tttcaaggcc
    27241 gtcttctcca cgacactcgt cattgacgaa aacctcaccg cggtatcgaa cagcctcgtc
    27301 gtctcggaaa cgcgtgagaa gggaaaaaaa atcgtgcgtt ttgccgacac gatcaagatg
    27361 tcgacgtatc tggtggcttt tgtcgttggt cggatggaag gcagtgctcc ttctgtcgtg
    27421 ggcaacaccc ggttacagtt gtggacagtg cccggcaaga cgcacctcac gagtttcgga
    27481 caggacattg ctgcagcgtc cctgcatttt ttcgagcgct attacgacat tccttatccc
    27541 ggcgacaaac tcgacctttt agccattccg gacttcgcct ccggagccat ggaaaacctt
    27601 ggcgccatca ccttccggga gactgcgctg ctcgtcgatc aacgaaccgg cacccacggt
    27661 gagctacaac gagttgcgga cgtggtcgca catgaaaacg cgcacatgtg gttcggggat
    27721 ctggtcacga tgtcctggtg gaacggactg tggttgaacg aagcgttcgc gacctttatg
    27781 gaaatgctgg cggtggacgc ctggaagcca gaatggaagc gctgggatgc ctttggtgcc
    27841 tcccgtggag ccgcctttgc cgtggacggg ctgcacagca cgcgtcccat cgaatttccc
    27901 gttcgtgcgc ccaaagacgc cgaagcgatg ttcgacgtgc tcacgtacga aaaaggcgcc
    27961 tccgtcctcc gcatgctcga gcagcacatc ggtccgaccg tatttcgtga cggcgtgcgc
    28021 gactatctcc ggacgcatgc ctatggcaat gccgacacaa acgacctctg ggtctccatt
    28081 ggcaaagcat cgaatcaggc cgtccccgac ctcatgaacg gatggatttt tcaacccggg
    28141 tatccgttgg ttaccgccga acttacaaaa gaatgcgaag tcgtgttgag tcaacaacgc
    28201 ttcagctatc ttcccgatcc gcctccggcc agctggcagg tcccggtgca gctgcgggtg
    28261 cagacaggag ccggagggga gagtcggcgg gtgttgctca ccggcacccg gttgaacgta
    28321 agctgtccac cagataccca attcattctc gtcaatgagg gtggacatgg attctaccgg
    28381 gtgcggtatg gaccgaactt gttgaaacgc gtgcttgaag ccggcctgga gaaactggcc
    28441 ccgatcgagc gattgaatct cgttaacgac gcctgggcga cgacgattgc cggcctgatg
    28501 ccactgtccg actatctcga cctgacgggc cgcttcgcgc tggagcgaga caagaatgtc
    28561 tgggccgtcc ttctcgattc cttcgccttc ctcaatcgaa tcatcgaacc ggcggatcga
    28621 gccggacttg aatcctttgt ccgctccaga gtcggcgagg ccgttgcggc tgtcggttgg
    28681 acaaccggac cgggggacaa cgacctgatg cgccagctca gaggtgaact gatcggcgct
    28741 ttggggcgat tgggcaatga ccaggcaacc caagcgcgcg ccactgagct ttacgccatg
    28801 gtccggaaag atcccgcagc aatcgatccg aacctcctcc ctgcattggt gtctattctc
    28861 gccttcatcg gcgatgcggc acgctatgag gactttcggg cgcgcttcaa gacggccgcc
    28921 acgcctcagg aggaacggag gtatctgttt tctttagcga tgttccagca gcatgctctc
    28981 ctcggccgga cattggacat gaccctcaac ggagagattc gtcttcaaga tgcccccttc
    29041 atggtcagtg cagtgatgaa caatgtctac ggacgtgaac agggttggcg cttcgtgaaa
    29101 atgaattggg atctgctgga ccgccggttt cccaagcaag ggctccgccg gatgtgcgga
    29161 gggatcaccg gtttctgcaa gccggagctt gaacgagacg ttcgtgagtt ttttcaggcc
    29221 cgtcaagtcg acttgggcgg gaaaacgctc gaacaatatc tcgagcagct ccggatcggc
    29281 gtttcattcg ccgaacgaag ccggcgcgat cttcaggaat ttctgcggag gaatcagtat
    29341 tcgcccgagg ttgctcaaac cagtgttccc ggttgagcca gccgaaatct catagtagtc
    29401 tggtaccatg tcgtcacgag ctggttcgat ctgcttcttc ggcgtcatgc tctgcgccct
    29461 catggccgct tgcaacggca gtgatgaggt tggaggcggc ggtgccggtc ccccccctgc
    29521 tacttccaat agtttgcgat tacagaccgt cacggcagtg ctttcttcgc cggtttttct
    29581 cacggccccc accggcgatg tgggtcgcct gttcattgtc gagcaaggcg gcttgatcag
    29641 aatcctgaac tctctggatg gcacgccgcg ggccactcct ttcctcgatg tggcaggact
    29701 cattgtcaca ggtggcgaac aaggtctgtt gggcatggcc ttcgatccca actacgccgg
    29761 gaacgggcgc ttttatatct actacacgaa tacagcaggc gatattgtca tcgctcgata
    29821 cggtgtcagc acgaatcccg atatcgcgaa tgccggcgca caggccattc tcaagaccat
    29881 cgcccacccc accaaccaga atcataacgg cggcatgctg gcattcggtc cggacgggtg
    29941 cctctacgcg ggcataggag acggcggtgg cagcggggat ccgaatggta atgcgcaaaa
    30001 taccaactca cttttaggca aaatcctgcg tctcgatcca gagacgggta gtgcctgcgg
    30061 caccgataac ccctttgcga acgggaccgg aggggctccg gaggtctgga gtttcggcct
    30121 ccgcaatccc tggcgcttct catttgaccg ctcgaccggc gtgttgtaca tcggcgacgt
    30181 tggacaggac cagcgtgaag aagtcgatgc cgtggtcggc cccaatgcag gtcagggtgt
    30241 caatttcggc tggaatatca tggaaggctt tgcctgcttc aatccgccga gcggctgtaa
    30301 cagcagtgga ctcacgccac cgatccttga ttacagccat gacgccgggg cctgttcggt
    30361 taccggcggc tacgtctatc gcggcaccct caatccggcg gtgaacggca gctattttta
    30421 tgccgattat tgcgctggat ttgtcagaag ctttcagctg caaggcggac ggccaagctc
    30481 acagaacacg tggccattac tcagcccggg aggtcagatc accagctttg gcgaggacgc
    30541 gcgtggtgag ctctacattc tgacgcagac cgggggactc tctcgcatcg tggcgaattg
    30601 acctgaagca ctgggcaagt ggaaagtgcc gccgtttcta ttcgtgccgc aggatggcca
    30661 gtggcctctg ccccaaaata cgaaatgtgc tgagtaagcc aacgaccaac gtgaggacca
    30721 tggtacacaa cagtcccgta accaggacca ttggatgcag cccccacgag aggtcgaaca
    30781 cgtaggtcag aacaagccag gataaggcgc tcgacaatac ggctgcaaga atgcccccga
    30841 cgacccccaa caagccatat tcgagagcaa agatcccggc aatcagactt cgcgttgcgc
    30901 ccaacgcctt caagaccacg gattcataca gccgccgata tctcgtcgcc gccagcgccg
    30961 ctgccaggac gaggcttccc gtcatgacgc agaacatcgc cacggcccga atggccaagg
    31021 cgagccgatc cagaacgctt gcaaaatttc gcaggacatc tcccacgtga atcgcgctga
    31081 cgttcgggaa tgccgccacg acggcctgct gtaacggcac ttcctgttcc ggatcgacat
    31141 ggatcgtggc cacatacgtc aaaggagcgc catccaacga gccaggcgac aggatcatgt
    31201 agaaattcgt cgaaaaattc ccccagtcga ccttgcgaat gcttgaaacc tctgcagtga
    31261 tagtcgcacc ttgaatgtct aattccactt gagaccccac gaccaggccg agggatttgg
    31321 cagcctcttc ctcaatcgaa acctgcggtc gcgaaaatgg ctggcctggc tgccaccacg
    31381 tgccggctac gacgtcatta cctttgggaa gctgatcaag gaacgtgaga acgtattcgc
    31441 gagagagata ccattgcttc cgacgctcct ccttgttttc atcacgtcgc tcttcgtgat
    31501 cggcctcctc ttcaaccttg acaacttcgc cgttgacagt atgaaggcgc gaccgcacca
    31561 gcggagtcat ttcaggttgg cgtcctcggg ttaactgctg gacgagcttg gcaaaccctt
    31621 cagtctgatc cacctgcaaa tcaataaaga agaacgtggg agcatccacc gggcgattgt
    31681 ctcccacctg tcgaagcaaa gcgtgttcca ccaaagaaac cgtcatgatc accatcacac
    31741 tgattccaat ggccaccagc acgccagttg tgtgtccacc gggccgtaac agattgccga
    31801 cggcatagcg taggctcaga gatgctggtc tcggcaccag actcaacgcc ctgatcagta
    31861 cgcgtgcaca gaggaagagc gtcagcatgg ctatagccaa tccgccgatg aacaatccgc
    31921 cgactttcca ggaaccggct tgccagatgg agagcagccc gaggccggct aaaatggctg
    31981 ccccgatcgc cacaggggcc tcgccattta tgccccatcg aatccaccac gtgcggcggc
    32041 ggttgtgttc gtcggggatg cctccctcca cctctcgccg aaaaatggca gcaggcctga
    32101 tctcacgcac tcgcaataac ggccacagcg cgaagagtac cgtggtcaat aggcccatgg
    32161 cgccgccttt caagagaggc ccgatcgaca cgaaagaaag ctcggccgag actcctaact
    32221 ggtcaaggag gtcgctcacg aaggcaccgg cgagcagtgg aggcagccag cgctgtaaca
    32281 agattccgag cacgatgcca actccactgc ctatcagtcc caagccgatg gcttgcacca
    32341 tatagacttt gatcagcacg gcggaatccg cccccagcgt cttcagaatg gcgatcgtct
    32401 tcatcttctc ctgcatgaac gcatggatcg acatggcgac acccaggcca ccgatgaaca
    32461 aggccgtcaa cccgatcaag ccgagataac gcgccagttg ctcgaggaac tgcttcagtt
    32521 ggggttgtgc gtcgcgatac ccggagatac gcacatggtc tgccgccagg cggccgcgca
    32581 attcagcccg cagaggttcc ggtggcattc cagacggcaa ccgtagaagc tgccgctcac
    32641 ggacacggct tccccgctgc acgagtcccg cagaccgcag cccggcctgc gagacgatga
    32701 cccgaggacc aagactgaag gcattggcca tccgatccgg ctccgttcgc acgagccccg
    32761 taatgagaaa ttctgctttc ccgagcttca atcgatcgcc gacccgaatt cccatgcgaa
    32821 gcaacaaggc ctcctggacg agaacgccat gacagcctcc gcatgccgta gaatccggat
    32881 cgagcaggcg ctccagcgga agggccggct ctactttcaa taggccgtac aagggatagc
    32941 ccggatcgac cgccttgagt tccacgatct gagtggtgtg tccggacccg gccgtatttg
    33001 gatgagccgc catggccacc agttcggtga catgggtcgt atcaacggcc cgctgatgca
    33061 gggagtcgac gatcgcccgg ctctcctccg tcaatggatg tgaggagcgg atttccaaat
    33121 ctccgccaag gaggcctttc gcctgtttgg agaccgcatc atcaacgtgg gtcgcgaaga
    33181 gggagacgcc caccagcgcg ctcactccca cggcaatgca gacgaaaaaa tacacaaaat
    33241 ggcgccaggc cccccgagtt tcccgccagg ccatcgagaa catgaaccaa ttcacaacgt
    33301 tggctcctgg tggctctccg tctcggatat gagatcgtcg gactcgatgc gcccatcgcg
    33361 cagtgcgatc acccgatgca tcaaggacgc gaccgccctg tcatgcgtca cgagcaccag
    33421 tgtggttccg taatctcgat gaagggacag caaaagatcc atgacgtggc gcccggtttg
    33481 cgaatccaag ttcccggttg gttcgtcggc aagaaggatc ggcgggcggc aggcaaacgc
    33541 tcttgcgacg gcaacccgtt gctgttcgcc cccggaaagc tgcaccgggt aatgatgcat
    33601 tctgtcctgc aagccgacgg catgtaagag ctccgccgct agatccttcg cagcggcgtg
    33661 gccggcgagt tccaggggaa cgagcacgtt ttcgagggct gtcaatgtgg gaatgaggtg
    33721 aaaggattga aaaatgtatc cgattttcat gcgtctgaat ttagccatcc ggctttccga
    33781 cagagtcgtg atatcgattc cgtccaggac gatggttccc tctgtcggcc ggtccagacc
    33841 cgcgatcagt ccgagcaggg tcgattttcc gctccccgat ggtcccatca ccgcaaccat
    33901 ttgtttcgga ggaatcgaca gggccacgtc gtcgagtatc gtcacccgtc gtccacctgc
    33961 cgtgagtcgc atggaaagat gtcggatggc gatcatagac ctcgctcctc aatgagaccg
    34021 actaaatgat attatactgg agtccgatga cactccgagc attagcccag gcattcgcgg
    34081 caagcttcat cactctgttc cttgcgacaa cctcgccctc catcacacac tctgcaggtg
    34141 ggggcagcaa caatgaccgt ccgcgcattg ttgcctttgg cgatagcttg acggcgggac
    34201 ttggcgtggg ggcagaagag acctaccctg cccagttaca gcgtcggctt gactctcgag
    34261 gcctccagta tcgtgtgatt aatgcgggag tgagcgggga taccacggca ggcggcctgc
    34321 ggcgagtaga ttgggtgttg aaaagccgcc cggacatcgt cattctggaa ctcggagcca
    34381 acgacggcct ccgtggcctc aagctgcaag agacgaaggc gaatcttgag aaaattatcc
    34441 aacggtgtcg agatgcatcg gccgtcgtcg tgctcgccgg catgaaactg ccacccaact
    34501 atgggacgga atatacaaca agtttcgaat ccatctatcc cgccttggca aaacagttcc
    34561 gattgccgct gattcccttt tttctggagg gggtggcggg atccacttca ctcaatcaac
    34621 cggacggtat ccacccgacc agcgaaggct accgcatcat tgcagacctg gtcatggaaa
    34681 cggtcacgcc tctgctcgta cgggaataga ggtgagttgg gactggagat catcgcccgg
    34741 gctcaacagc gctgagcccg gggatcacga aaagacaggg ttagctcttc ttgaagaatg
    34801 tgtcgtacac gccgtaggcg agcaccaatc cgatgagtgc gtagtacatg gtactgatgc
    34861 cgctatagtc cggggcccca cccttcggct cgttggccaa cacggaggag gcgccaacga
    34921 tcaccaacat accggcagct gcactgaccc gtccccacat cttgatcata tctgtccctc
    34981 cctcgatgga atgactcaaa cgagggcgca ttgtactgaa atcgcttggc ggggcgcaag
    35041 aggattgacg tcgccggggc aagtctctga atgtgtttat ggggtcgagc ctggtttcgc
    35101 tatggtcttc aacgcgacgg ccacctctcg aaacgaattg gcaagcttat caagctgggt
    35161 gcctttcgag atcagttcac ccgcgattgt cttcatctga ctgtcatatc cgcccacctc
    35221 tgaagcaatt tctgcaactt tgacgaacat gtcttgatag gcggcaactt cctgttgaag
    35281 gtgctgtaac tgtgactgag ccgctttgac ttcggccatc gaggcctgaa gttgctgagt
    35341 catccgctcc acgtacgctt cgcgctcccg ctgctcggat tccagtgtca actgaatctc
    35401 ttcactttca cggcgtcgtt catccagggt ccggatggtc tgcacccagg cggcaaggtc
    35461 gccgctcaga tgcgactgct cgggaagcgg ttcgcccttt tccagcgcct tcatcgtcgg
    35521 attcaaccac tcgataaaag ccatgacctc gcttaacttg agatccgccg tgaaattcga
    35581 tggaggaacg gtatttccat tggaagcttc agaagacatc gggaccggat tttgcctcgc
    35641 ctttttcgtg cgcggctgca cccatcgatg gtattccaaa agaacggcaa tgcaatggcg
    35701 gcacatcggc tcttcgggca acgaacaact gcacctcgac accaaatggc cgtctttcaa
    35761 tcgaatggtc tgctcgtaga ggccggagtt gccgatcacg gccgaggaga tctgcgcctc
    35821 attggcttcc acgatgcgca cgcgattttc cgacaggtac tgactgccga gatggaaggc
    35881 gttgcggtcg acgacacctt ggatcatcgg aggatccaag agactcaacc gttcggcgga
    35941 acaggggatc ttgtttcgca tgcagatggt cccacaccgg gaacgacgcg ctgccggaaa
    36001 ctctcaacgc acgcccctag tgtgaaacgc catgccaaaa gtgtcaagga cggagcgatt
    36061 ctacgcagga ccgggtcgat tcagcggcaa cggcggggca tcccctcgcc actcacaggc
    36121 gtgagccaga cataatcggc gatcttgcca tcaagcatct cacgcacgtc gtcctgtcgt
    36181 ccccgctcat atgacgtcat gtaaagactg gtttgccgga cgttaccgga taaccgccgc
    36241 acaactcgat tggcaaccgg caggttgtat tgcacgtgtc ccccgcccgt atagctgacg
    36301 acaggaccgg ccaacgcatc cgaccgttca cgtatggact cgaccaacgc gacgactgtt
    36361 cgagccatgc cctcatcacg caccatcgac gcttcgtaca tcgttcggta catgtcgtcg
    36421 gacccgccgc catgacaggc acggagctgg tcaaaaatcc tttgccgata cagcggatcg
    36481 tctacgatcg tttcatcctg catcttccac cgcttccaat ccgcctcctc cctggcgtgg
    36541 tcgagacctt ccttcgccac aagcctcacg agagatttag gcggattgag cgccaccaac
    36601 cccagacgct catgtttggc gaaggccaca agcggctcat aggtttcaaa cgcccctccc
    36661 cagttctggc tccatcccac ctggctcaaa aactcttggc gacttaattc ggcccccccg
    36721 acataccggt ccagcaaggg ctgagcatcc caaccgaaca tctccatagc caggaccggc
    36781 cggcggccag ccgcagaaag accattcaag acggacaagg cggaattgat gtggaagcga
    36841 ttatgatgtt cctctcccag atagacgacg tcttgctgga gcagatgact gaccagctgc
    36901 tccaaagaaa tgaattggcc gttcgccgtg tcgataattt ggccttctcg gaattcggtg
    36961 gatggaggcg tcggagacac tgacgcgtcc gttgctgcgc agccggcagt catccacagt
    37021 agaccgatga gtagtccatg tccgatcata aaatgcgcac tccgggatag aagatcctct
    37081 cgccgattct gctctttttc cggcataggc cgcaaggcga ccgtccttga cccttcggtc
    37141 tatggccggt atgctgcccg gcgtgtgatg acaagtcacg ccgtcttgtt gatcgcatct
    37201 ccatattttc atgctctctc acgcgcagtt gctggcagca tttcgctcgc aaattaccag
    37261 gctcttacaa gaacgcgata cgcaatggga gcgctcgcgc cgtctgatcg aagctgccag
    37321 tttctcgggt acggtacgca gtctggtcga acgtgcccag cacacggatt tgcccgcgcc
    37381 tgtgcgagat gcactgctta cagccttgaa cgagggccag gtcgccagga tccaggacct
    37441 gtcgggaccc cgtctcaaag agctgaccgg cctcccgccg agcaaggccc tccgggcatt
    37501 gtgcgtttgg tttggtttag ttgaggacgc gccttcacgg tggccagtgt cgtcactcga
    37561 cggcgaatcg gtcgcgactt ttctccatac acattcgaac ccttttgatt tgcttgtcac
    37621 ggcagacgtc ccttccgtgg tggaattcgg cgccggcgat ctatcgtttg ccaccgaact
    37681 cgccgaccta tacgcacccc ggatctgtga gcataaccga acactcctgc tccattgcat
    37741 cgaccgtctc agtcctcagt ccaaattggg cggtccactg catccaagtc aggaccggtt
    37801 gcaggcgctt cgttcgagaa ccgacgtgtc atttcaattc ttcggggatc aggacatgtg
    37861 cggcttcgat gagctggccg aagccggacg gctcgcgtct cgatatgccg tggccgtctg
    37921 ttgggccccc gcgactccta catttgccta tgaacccacg cggctttccc ctgaagtgat
    37981 cgagcgggag ctggtgcgga cgaagggacc gtaccgtcac gcgcagtacg cgggtgagcc
    38041 ggccctcgaa gtacacaatg gagatcggac gctcctgttt ccaccatgga agttcgacat
    38101 tcatggtccg ctcgtcttat taaaccttct agcgcgcggc ggccagctgg gagttctcgg
    38161 tgccgtcgac aatcaggtgt tctgggaact gctggcacaa ctgttggagg atgagcgata
    38221 ccgtccagaa aatgagccct ttactcccga aaatctgtcc acaattttca acccggttta
    38281 cgagccgctt gccacactgc caatcggcga aagcctcgag ctggcaagct gtggaacact
    38341 tcgtcgcagt atcccgcgcg tcttgccttc caaacgactg ccggagacct atcggttccg
    38401 ctccgtgttg attcgccgtg gcgcatgctt tcccggcatg cctgccagcc atacggcgag
    38461 acgattctcg gacatggtcg aagaagcctc tccctggatg atgacactcg ctcctgctgt
    38521 gtgaagatct atcgggcggt gcgggcatca ccgcgtaaat tcgccgaaaa attgaccgcc
    38581 actagaccct ttgttacact tcaacggtgt cagtcaacca catattctcc agccgctaga
    38641 gccaataatc tgccatgaac gtcccgaaac cttttcacgc gattcaggac aatattgcgc
    38701 gagtcattaa gggcaaggct caggtcattg agatggccgt cgtctgcctt gtggcgcgcg
    38761 gacatttgct cctcgaagac gtcccgggag tcggcaaaac caccctggcg catagcttgg
    38821 cccgctcact cgactgttcg ttcaaacgga ttcaatttac cagcgatctc ctgccctccg
    38881 atattgtcgg ggtgtccatc ttcaaccggc aacatcaggc gtttgaattc gtgcccgggc
    38941 cgatctttgc gaatatcgtg ctggccgacg agatcaatcg cacgacgccc aagacacaaa
    39001 gcagcctctt ggaggcgatg agcgaagcac aagtatcggt ggacaaccaa acgcattccc
    39061 tgaaacaacc cttcatggtc atcgccacac agaatcctgc cgaatatcac ggcacgttcc
    39121 cactgcccga atctcaactg gatcggtttt tgatgcgctt acgcatcgga tatccgtcat
    39181 tggaagaaga gcgcaaggtc ctcaaccgcc cttatgcgct gcatcctgcg gacagcctcg
    39241 ccccggtgct cacggcgcag gacgtgctgg aattgcagcg gcggatcgag gaggtgcata
    39301 tggatgagag cgtctgcgaa tacctgctgg ctattgtcca tgcaacaagg cagagcgagc
    39361 tcctgtctct cggcgtcagt accagaggag ccttggcact caacaacgcc tcgaaggccc
    39421 tggcacttgt gcgcggccgc gagtattgct tgcccgacga cgtcaaagaa ttagccccga
    39481 tcgtcttgtc tcatcgcgtc atgctcagcc gagcgcaagg catgcgtgct caaagctttg
    39541 agcaggccga gcgcatcgtc ctcgacattg tcgaaacgat tcccgtcccc gtgtgatgct
    39601 gcaggacagc acgcctgtga cgaaggcatt cgggcacaat cccgccctcc ggatccgatc
    39661 ccggctgcac tatttagcca cccgtcgttc catccgcctc acctctgaag gcatccggtt
    39721 cttactcttc accatcggca tcggcgtggc ggccatcaac acaggcaaca atctctttta
    39781 tctcttgctg gcgatgatgc tcggcctcgt ggtgatttcc ggtctcctgt ccgagcactg
    39841 tttgcgccgc ctcgagtttc accgccatct gcccgatttg atcatggcga acgagccaac
    39901 gaccgtgacg ctctcggtca cgaatcacaa ccgctggctg ccgagcttct ctttacggct
    39961 atccgatatc gtgagcggac agcaactgga ccggggctta ttcattgatc acttggcggc
    40021 acaacgatcg gtcctgctct cttacccgat cgtagcgacc acccgcggca agctgtctct
    40081 cgactgtgtg agagcggaaa cgctttttcc cttcggcctc tttctcaagc gggcactcta
    40141 tcaggtccct gccgaaattc ccgtcagtcc gccgatcaaa ccggtttcac ttcgcttcat
    40201 cgatgaaatg gtcagcgagg gacaaggcct gtcgcttccg cgccggggac atggaacgca
    40261 gttgtacaac ttacggctct atcagcccgg cgatgattcc cgggcgattc attggatgac
    40321 cactgcccga acctctaccc tgatggtccg ggagaccgaa gccgaggatc agcggcgaat
    40381 gacgattgcg ctttcgacgc tcgcaccgga cgagcggaat acgctgttcg aacacagcgt
    40441 gaccctggtc gcatcgctgc tctggcactt gtcccagaga tccttcccgc tccggctgat
    40501 cgtccatgaa gcggattccg ggctgggctg cggctccgat cacctgttcg ccatgctgcg
    40561 gctactcgcc gtttgtgaac gacgtgctcc agattccaca ggtcctacag tctccaatcc
    40621 catcctgaca ttgggccatg atgccgagcg cggctacacg gtggttgttc tgccgtggag
    40681 cgatcccgag gcttcagcgg ctgtgtccgc cgatcgcgtg cttcaagctg cccacattga
    40741 aggacttacc aatggttttt aaccaggcct gtcgattcag cgcgattgtc cttgctgcaa
    40801 cgtcgtttgc cgggctgctg ttcgcacgca gcatgccgag ttggctcgca attctgacag
    40861 tgaccgtcct tgtcttcgcg attctacaga cgttcggatg gccgtcgggc aatacagtcc
    40921 tcagtggcct ccccaacgcc ccgtccgtgt ggaatagtct gttgattggc gccttcgttt
    40981 tgtttctgct ggacctgacg atgatatcgc gcgagctgct tccggcgggc attcacttcc
    41041 ttgtcgtact tttgaacgtg aagctcatga cgctgcatga tcggcgcgac taccgtcatc
    41101 tctacgccat ttccctgatg gccattcttg catcggccgc cttgacgacg gacgtctggt
    41161 atgtgtcaat tttcgtgttc tatctgcttg cggtggtgtg gagtctgctg ctgcaccatt
    41221 tgaccagcgg gccaccctcg gccaatccgg cgcgcgcgca ggtttcgcct cgcgccacct
    41281 ggttcatca
//
LOCUS       DASSCQ010000102         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1591942, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000102 DASSCQ010000000
VERSION     DASSCQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591942"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /gene="modA"
                     /locus_tag="VFH05_07410"
     CDS             <1..801
                     /gene="modA"
                     /locus_tag="VFH05_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6678077.1"
                     /translation="GRRLLLGFFILTLLAPQYALAQPETLTIAAANSVKDALRKMLPL
                     FEAEHPHIAVRVIYGPSQTLRKQIEEGAPVDVFLPSLFDEIEQLEKKGLVIQGTKRVY
                     AGTSLVLITGTVLPAPIRSIEDLHTVPVHHIAVGDPKTSSVGKVAAQFLKYSKLEPAL
                     KSQYVFGEHSRAVLDLVSKGEAEIGVVYRTDAVADDHVRIVDTAPAESHNPVRYGVAV
                     VWTARNISGAGDFIEFMLSAPIQAQLKQYGFDQAVSDAGLVRRQEVKP"
     gene            798..1529
                     /locus_tag="VFH05_07415"
     CDS             798..1529
                     /locus_tag="VFH05_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678078.1"
                     /translation="MTKRTRIDTQSPKWSAALLVLVASMIGAGCAGTSDPPPKTIYES
                     GRNQVRLVKDPDSSTNAHPATLSAAEVGTLLRSVRSWERRSFVHRLFSGEADKTRAFR
                     NEEITVLAPALSKALAQAAPDERAYFHLSHATDQGDEESTTGWLSIRNGTLHLSLSEV
                     HDRHGPEPDISKYDRQMPNVRERSAEFDVTFEPEDYLIKVKSAGKLFAPDQREELQIK
                     YREALAALPTYPGLEPRQPSQPSQP"
     gene            1580..>2183
                     /locus_tag="VFH05_07420"
     CDS             1580..>2183
                     /locus_tag="VFH05_07420"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HET6678079.1"
                     /translation="MDASKSTRPHIGIVAGTADGASLCYRSLCYEAVRTGRPAQPEIT
                     MHTFPLEWYLNLIERDDWTGVAALMSRSAGKLIQAGAELIICPNNTLHRAYEHVVSAV
                     PWLHIVEAVTAEIGRMGLRRVGLLGTAGVMEGRIYHPRLSQAGIEVVLPEEHERSRLH
                     HIIGTELIRGQYTSPSRLYVQEMIEILAARGTEAVILGCTE"
BASE COUNT          474 a          646 c          625 g          438 t
ORIGIN      
        1 ggacgacgat tactgctcgg ctttttcatt ctaacccttc tggcaccaca gtatgcactt
       61 gcacagccgg agaccttgac cattgccgcc gccaacagtg tcaaagacgc cctgcgtaaa
      121 atgctgccgc tgtttgaggc ggaacacccc cacatcgccg ttcgtgtgat ctatggccct
      181 tctcaaacgc ttcgcaagca aattgaagaa ggcgctcctg tggacgtctt ccttccctct
      241 ctgtttgatg agatcgaaca gctcgagaaa aaagggctcg tcattcaagg cacgaaacgc
      301 gtgtatgccg gcacctcact cgtgttgatc acaggcaccg ttctccccgc ccccatcaga
      361 tcgatcgagg acttgcacac ggtgcccgtg caccacatcg ccgtcggcga tcccaaaacg
      421 tcgtcagtcg gtaaggtggc agctcaattc ctcaagtaca gcaagctcga gccggcactg
      481 aaatctcaat acgtctttgg ggaacactcc cgtgcggttc tcgacctcgt gtccaaagga
      541 gaagccgaaa tcggcgtcgt ctatcgcacc gatgccgtgg ctgacgacca tgtgcgcatt
      601 gttgataccg cacccgctga aagccacaat ccggttcgtt atggcgtcgc agtggtgtgg
      661 acggctagaa acatttcagg ggccggtgat ttcatcgagt ttatgctctc cgcgccaatc
      721 caggctcaac tcaagcagta tggattcgac caggcggtat ccgatgccgg gctagtccgg
      781 cgacaggagg tgaaaccatg acaaagcgga cgcgaatcga cacccaaagt ccaaagtggt
      841 ccgccgcgct gctcgtcctg gtcgcatcga tgatcggtgc cggttgcgcc ggcacttccg
      901 acccgccgcc aaaaacgatt tatgagtcgg ggcgaaacca ggtccgtctg gtcaaagatc
      961 ccgattctag caccaacgcg catcccgcca cactttctgc tgcggaagtg gggaccttgc
     1021 tccggagtgt ccggagttgg gagcggcgca gttttgttca ccgcctgttt tccggagagg
     1081 ctgacaagac ccgagcgttt cgcaacgagg agatcacggt cctggcgccg gcactctcaa
     1141 aggcactcgc gcaggccgca cccgacgaac gcgcctattt tcacctcagc catgccaccg
     1201 accagggtga cgaagagagc acgacgggat ggctgtcgat tcgaaatggc acgttgcacc
     1261 tttctttgag cgaagtgcac gatcgccacg gaccagaacc ggacatcagc aagtatgatc
     1321 gccaaatgcc gaacgtccgg gaacgatcgg ctgagtttga cgtgacattc gagccggaag
     1381 attatctgat caaggtaaag tccgctggaa aactctttgc tccggaccag cgtgaggaac
     1441 tccagattaa atatcgagaa gcgcttgcgg cgcttccgac ctatccgggg ttggaaccgc
     1501 gtcagccgag tcagccctca cagccctgaa tgtgtgcggg cctgccgctt gtcgctgtgc
     1561 agactcaagg agataccacg tggacgcatc caaatcgacc agacctcata tcggtatcgt
     1621 cgcgggaacc gccgacggtg cttcgctctg ctaccgcagt ctctgttatg aggctgtgcg
     1681 tacaggccgg ccggcccagc ctgaaatcac gatgcacacg ttcccgctgg agtggtatct
     1741 gaatctcatc gaacgggatg actggaccgg cgtggcagcg ctgatgtctc gatcggcggg
     1801 taaattgatc caagcaggag ccgagctgat catttgcccg aacaatacgt tgcatcgcgc
     1861 gtacgagcat gtggtatcgg cggtcccctg gctgcacatt gtggaggcgg taacggcaga
     1921 gatcgggcgg atgggactgc gccgcgtggg actgctcggc acagcagggg tcatggaagg
     1981 gcgcatctat catcctcggc tcagccaagc gggaattgag gtcgttcttc ctgaggagca
     2041 tgagcgcagt cgcttacacc acatcatcgg aaccgagctc attcgggggc agtacacctc
     2101 gccttctcgc ttgtacgtgc aggagatgat tgaaatccta gcagcgagag ggaccgaagc
     2161 ggtcatcctc ggttgcaccg aac
//
LOCUS       DASSCQ010000103         7080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1344116, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000103 DASSCQ010000000
VERSION     DASSCQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344116"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1285)
                     /locus_tag="VFH05_07425"
     CDS             complement(323..1285)
                     /locus_tag="VFH05_07425"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HET6678080.1"
                     /translation="MAAFWSDKRVVVTGGAGFLGSFLVEHLRAKDCRAVVVPRSKDYD
                     LVEMDAVRRLYADAKPDVVIHLAARVGGIGANQANPGRFFYDNLMMGTQLIEVGRQLG
                     IKKFVAIGTICAYPKYAPIPFKEDDLWAGFPEETNAPYGLAKKMMLVQSQAYRQQYGF
                     NSIVLFPVNLYGPRDNFDLETSHVIPALIRKCAEAQQNGKPEIVLWGDGTPTREFLYV
                     EDAAEGILLAAEQYNDSRPLNLGTGEELSIKTLAGIIAAEVGFHGRILWDPTKPNGQP
                     RRCLDISRIKQTIGFQPTHSLRDGLKKTIQWYRANLDALREVRL"
     gene            complement(1288..2298)
                     /gene="gmd"
                     /locus_tag="VFH05_07430"
     CDS             complement(1288..2298)
                     /gene="gmd"
                     /locus_tag="VFH05_07430"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET6678081.1"
                     /translation="MLVKKALLTGITGQDGSYLAEFLLKKGYEVYGIIRRSSSFNTGR
                     IDPFYEDPHVPQRRLHLVYGDLNDASSLNRILRTVQPDEIYNLGAQSHVRVSFDIPEY
                     TGEITGLGTIRLLEAIRESGLRPKFYQASSSEMFGKVLEVPQRETTPFYPRSPYGAAK
                     VYSYWITVNYREAYNLFACNGILFNHESPRRGETFVTRKITKAAARIKLGVQQDLYLG
                     NLDAKRDWGYAGDYIEAMWLMLQAERPDDYVIATGETHSVRELLELAFDRLQLNWKNH
                     VKIDEKYYRPTEVDLLIGDASKAKRELGWAPKVGFHELITRMVDADLAAERERLDGIR
                     GK"
     gene            2502..3377
                     /locus_tag="VFH05_07435"
     CDS             2502..3377
                     /locus_tag="VFH05_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678082.1"
                     /translation="MITSRRVLILIFILGVTFLIAHTINAVIAEALYVPSGVALPRAV
                     DQDSAASHSPAALAEQVRMSGLFPLPADPVAAVGGTISGGPAGVPMRQALNLASKIKV
                     LGVVVGDRDGISAIIEELGSKRQLFLRLHDEIPDIGELSEIRRNGIVVRQGNQQEFLE
                     LSTGQEKPPMPQPVSTGPAASGGGSQIKKVLDRREVEQALNDLPKLLSQARAVPVLSN
                     GAMTGFRLDYIAPSSFYEKIGLHYGDVLKQVNGVEIKDPGTMLSLFQQLRNEKSVKLD
                     VLRNNQRTTMLFEIR"
     gene            3567..5294
                     /gene="gspE"
                     /locus_tag="VFH05_07440"
     CDS             3567..5294
                     /gene="gspE"
                     /locus_tag="VFH05_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HET6678083.1"
                     /translation="MAKQTPTRPLLGAILEQKFGLSEAKLLEALNIQQTKGGRLGEAL
                     IKIRAIEEDLLLQALAMQFEMTWLSHLDVKQVDQELIKKVPIHFARRYRVLPLRVDDG
                     AIVVATIDPLETAALDDLRLLLGMPVKPVLTGAMSLMACLNRAYDEAASPTGAEQVME
                     DIAASENLDKIAHELDEPQDLLDATDEAPIIRLVNSVLFQAVRQRASDIHFESFERGL
                     VVRYRIDGVLYPILTPPKHLQSSIIARLKIMAGLNIAEKRLPQDGRFAIRTAGKDVDL
                     RVSVLPTSHGERVVLRLLEKENRLLNLSEMGFSPDRLRTIQQLIRLTHGILLVTGPTG
                     SGKTTTLYAALSEINAPDKNIITVEDPVEYQLVGIGQMQVNPKINLTFAAGLRSILRQ
                     DPDVIMIGEIRDRETAEIAIHASLTGHLVFSTLHTNDAASAATRLIDMGIEPFLVASS
                     VVAVLAQRLIRKVCPDCKQAYHPDDEELIRLGVVPGKMKPTFYRGTGCPACTQTGYRG
                     RTGIHELLVLDDEVRRLIGNKADAAAIRQAAVSKGMVLLKEEAAEKIFAGITTTEEVM
                     RMTQQEVEV"
     gene            5298..6536
                     /gene="gspF"
                     /locus_tag="VFH05_07445"
     CDS             5298..6536
                     /gene="gspF"
                     /locus_tag="VFH05_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HET6678084.1"
                     /translation="MPVYQYRGYKADGASATGIVDAESPKVARLKLRKDGVFPTEMAE
                     QEGATKTVIPRSSSGLSAGVARSAVLTVTDVALMTRQLATLLVAGLPLVDALGVLMDQ
                     TEKKGVKGLLADVREAIRGGASYSVVLERYPRDFSSIYVHMVRAGEASGALDQILFRL
                     AEFLEKQLALKNKVTNAILYPAIMLVVGTLVLFFLVTFVVPKITAVFAHSKQALPWPT
                     IVLMEISQFCSDYWMVLLAGILIGIWGLRRFKKTETGRVLADRLLLQLPLIGQVARMV
                     SISRLASTLATMLSSGVQLLEALDVSRRVMNNRVLEQAVEEARQNIREGETIAEPLKR
                     SGQFPPLVTHMIAVGEKSGEMEEMLRRIGQIYDGEVDRVITRFTSLLEPIMIVVMGVV
                     VLFIVVAILLPIFEMGQMVR"
     gene            6561..7061
                     /gene="gspG"
                     /locus_tag="VFH05_07450"
     CDS             6561..7061
                     /gene="gspG"
                     /locus_tag="VFH05_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET6678085.1"
                     /translation="MRAQTHTVQDGTAVFRSERGFTFIEIMVVVAILAILAALVVPRI
                     MGRTDDAKRTAAKVQIRNIEGALQLYKLDNGVYPSTEQGLKALVEKPSVGVVPKKWKL
                     GGYLPSIPEDPWGNPYKYLSPAPVQQGQYGQIKADYEITSYATDGEAGGEGVNADITN
                     WNMEKD"
BASE COUNT         1523 a         1979 c         2040 g         1538 t
ORIGIN      
        1 tttgagcgca taggtccggt cttccgccgc ctgttcaaat tcttggatgg cctgcttgaa
       61 cagccccgtt tttctcagag ccactccgcg ttcatatttt tcagcggcat ctaccctggc
      121 ttccatggat ccttacgcag caataatggt ggtaaacgtg gtccttgcat tcccgagttg
      181 agcaaaagcc ggaccacaaa tggccaaatt aacaaatctt tatgggtcaa cgggttagca
      241 cgcagtcaga aaggatggat ggccgtcaag acactttctg taatccgacg cgcataaatg
      301 cccgaagcat gcaacgctcg aattacaatc ggacttctcg taacgcatcg aggttggctc
      361 ggtaccactg gatcgtcttc ttcaggccat cacgaagtga atgcgtgggc tgaaagccga
      421 tggtctgttt gatgcggctg atatccaagc aacgtcgggg ttgtccgttc ggcttggtgg
      481 gatcccatag gattcgtccg tgaaagccga cctcagccgc gatgatgccg gcgagggtct
      541 tgatcgagag ttcctcccct gttccaaggt tcagcgggcg tgagtcattg tattgctcag
      601 ccgccaggag aattccctcc gccgcgtctt cgacatagag gaattctctg gtcggggtac
      661 cgtctcccca aagtacgatc tcgggtttcc cattctgttg agcctccgca cattttctga
      721 tgagtgcggg aatcacgtgt gaagtttcga gatcaaagtt atcgcgaggc ccgtacagat
      781 tgactggaaa cagcacgatt gagttgaatc cgtactgctg ccgataggct tgcgattgga
      841 cgagcatcat tttcttggcc aggccgtagg gcgcattggt ctcctccgga aagccggccc
      901 acagatcgtc ttccttgaac gggataggag catattttgg ataggcgcag atcgttccga
      961 tggcgacgaa ttttttaatg cccagctggc ggccgacttc gatgagctga gttcccatca
     1021 tgagattgtc atagaaaaac cgtccaggat ttgcctgatt cgctccgatt ccgccgaccc
     1081 gggcagccag atgaatcacc acgtcgggct tggcatccgc atagagacga cggacggcgt
     1141 ccatctcgac gagatcatag tccttgctgc gggggaccac gactgcacgg cagtcttttg
     1201 cgcggaggtg ctcgaccaga aacgagccca agaacccagc cccgccggtc actacgacgc
     1261 gcttgtcgga ccagaatgcg gccactgtta cttccctcta attccgtcca gtcgttcccg
     1321 ctcggcggca aggtccgcgt caaccatcct cgtgatcaat tcatggaacc cgaccttggg
     1381 agcccatccg agttcccgct tggctttact ggcatccccg atcagcagat ccacctcggt
     1441 aggacgatag tatttttcgt cgattttgac atggttcttc caattcaact gaagccggtc
     1501 gaaggcgagc tcaagcagtt cacgtacgct atgcgtctca ccggttgcga tgacatagtc
     1561 gtcgggacgt tctgcttgca gcatcagcca catcgcctcg atgtagtccc cggcgtatcc
     1621 ccagtcccgc ttcgcatcga gattgccgag gtagaggtct tgctgcacgc ccagcttgat
     1681 gcgcgccgcc gccttggtga tttttctggt cacgaacgtt tcgccccgtc gtggcgattc
     1741 atggttgaac aaaatgccat tgcaggcgaa cagattgtag gcttcgcggt agttgaccgt
     1801 gatccagtac gagtacacct tcgcggcccc gtaaggactg cgcgggtaaa aaggggtggt
     1861 ttcacgttgg ggcacctcga gcactttccc gaacatttcg ctggaggatg cttgatagaa
     1921 cttgggtctg aggccggatt ctcgaatggc ttccaagagg cggatcgtgc ccaggccggt
     1981 aatttctccc gtatattccg gaatgtcgaa gctgactcgg acgtgactct gggcgccgag
     2041 gttgtagatt tcgtccggtt gaacggttcg cagaatgcgg ttgagcgaac tggcatcgtt
     2101 caggtcgccg tacactaaat gcaggcggcg ctgcggaacg tgcgggtctt cgtaaaaggg
     2161 atcgatacgg cccgtattaa acgaactcga gcgtcggata atgccgtaca cctcgtaccc
     2221 tttcttaagg agaaattcag caaggtagga cccgtcctga ccggttatcc cggtaagcag
     2281 cgcttttttc acgagcatct cctttggtta agaaattatg gatcgaaccg tatggattgt
     2341 ccactgagtc agtcatctca tgcggtctat cagagccggg agaaggtcat tcctgcctct
     2401 cgctgttagc ccgaccgtgg tcaaaatccg gccatctatt gcgcctaccc acgaggcaca
     2461 ttacaatacc caacgatgtc cgcggagctt tgcgctttcc catgatcacc tctcgtcgag
     2521 tgctcattct gatttttatc ctcggcgtga cgttcttgat cgctcatacg atcaacgccg
     2581 ttattgcgga ggcactgtat gttccatccg gcgtggcact gccgcgagcg gtcgatcagg
     2641 acagcgccgc ctctcattcc cccgctgcgc tcgccgaaca ggttcgcatg agcggcttgt
     2701 tcccacttcc cgcagacccg gtcgccgcgg tgggagggac catatcgggc gggccagccg
     2761 gagtacctat gcggcaggcc ttaaaccttg cgtccaagat caaggtgctt ggcgtagtgg
     2821 ttggagaccg tgatggcata tcggcgatca tcgaagagct tggcagcaag cgccagctgt
     2881 ttctccgcct ccatgacgag attccggaca tcggagagtt gagtgagatc cggcgaaacg
     2941 gcatcgtggt ccggcaaggc aatcagcagg aattcctgga actgtcgacc ggacaagaga
     3001 aacctccaat gccccagcct gtgtcgaccg gaccggccgc gtctggcgga ggaagccaga
     3061 tcaagaaggt cctggatcga cgagaagtag aacaggcctt gaacgatctc cccaagctgt
     3121 tgtctcaagc ccgggccgtt ccagtgctct cgaatggggc gatgacggga tttcgtttgg
     3181 actacattgc cccttccagc ttctacgaaa agatcgggct gcactatgga gatgtgctca
     3241 agcaggtgaa cggcgtggaa atcaaagatc ccggcacaat gctctcgtta ttccaacaac
     3301 ttcgcaacga aaaatccgtc aagctcgacg tcctacggaa caatcagcgt acgacgatgc
     3361 tgttcgagat acgctaaggt cagccgggct gacatccttc cgacctctct cgttcactgg
     3421 gtatatctcc tggtttggct gccagtcagc cggcagcttg tcagagccgg tcggagagga
     3481 gtaatttagt agtttcggtg gcttaggccg attgtaggca atccagaggg ataggagatc
     3541 ccctagaaat agacagcggg catactgtgg cgaaacagac tccaacgaga ccgttgcttg
     3601 gcgccattct ggagcaaaag tttggcttat ccgaagccaa gctcctcgag gcgctgaaca
     3661 tccaacagac caagggaggg cgtctgggag aggccctcat caagatcaga gcgatcgaag
     3721 aagatctgct gctgcaagcg ctcgcgatgc agttcgaaat gacctggctt tcccacctcg
     3781 acgtcaagca agttgatcag gaacttatca aaaaggtgcc gattcatttt gcgcgccggt
     3841 atcgggtgct gccgctcaga gtggatgacg gagcgatcgt cgtcgcgacc atcgatccct
     3901 tggagactgc cgctcttgat gatttacggc ttctcttggg gatgcccgtg aagcctgtac
     3961 tcactggcgc catgtccctg atggcctgtc tcaatcgcgc gtacgacgag gctgcgagtc
     4021 ccaccggtgc ggaacaggtg atggaggaca ttgcagccag cgaaaatctc gacaaaatcg
     4081 cccatgagct ggacgaaccc caggacctcc ttgatgcgac ggacgaagcg ccgatcattc
     4141 ggttggtgaa ctcggtgctg ttccaggcgg tccggcagcg agcgagtgac atccatttcg
     4201 aatcgtttga acgtggactg gtcgttcgct atcgcatcga cggcgtgctc tatcccattc
     4261 tcacgccgcc aaagcatctg cagtcgagca tcatcgcgcg tctcaaaatc atggcaggct
     4321 tgaatattgc ggaaaagcgg ttgcctcagg acggccgctt tgccatccgg acggccggaa
     4381 aagatgtgga cctccgcgtg tcggtgctgc cgacatcgca cggcgagcgc gtcgtgttgc
     4441 gcctgcttga aaaggaaaat cgattgctca acttatcaga aatgggcttc tcgccagacc
     4501 ggctccgcac gattcagcaa ctcattcgcc tcacacatgg gatcctcctc gtgacaggac
     4561 cgacgggcag cggcaaaacc acgaccctgt atgcggcctt gagcgaaatc aacgcgccag
     4621 acaagaacat catcacggtc gaagacccgg tcgaatacca actggtcggc atcggccaga
     4681 tgcaggtcaa tcccaagatc aatctgacgt ttgcggccgg gctgcgatca attctccggc
     4741 aggatcctga cgtcatcatg atcggcgaaa tccgcgaccg agaaacggcc gagatcgcca
     4801 ttcatgcgtc tctgacgggc cacttggtct tctccacgct gcataccaat gatgccgcaa
     4861 gcgcggccac ccgcctgatc gacatgggta tcgagccgtt ccttgtcgcc tcctcggtgg
     4921 tggcggtcct ggctcagcgg ctgatccgaa aagtctgtcc ggactgcaaa caggcctatc
     4981 acccggacga tgaagaactc atcaggctgg gcgtggttcc cggaaagatg aagccgacgt
     5041 tttatcgtgg caccggctgt cctgcctgca cgcaaaccgg gtatcggggg cggaccggga
     5101 tccatgaact gctcgtcctg gacgacgaag tgcgccggtt gatcggcaac aaggcggatg
     5161 cagccgccat cagacaggcg gcggtctcga agggcatggt ccttctcaag gaagaagccg
     5221 cggagaagat ttttgccggc attacgacga ccgaagaggt catgcgcatg acgcaacaag
     5281 aggtcgaggt ctagcggatg ccggtctatc aataccgggg ctacaaagcg gacggcgctt
     5341 ctgctaccgg cattgttgat gccgaaagcc cgaaagtcgc ccgactcaag ctgcggaaag
     5401 acggcgtctt ccctaccgaa atggcggagc aggaaggcgc gaccaagacg gtcatacccc
     5461 gctcctcttc agggttgagc gccggtgtcg cccggtccgc cgtgctgacc gtgaccgacg
     5521 tagccttgat gaccaggcaa cttgccactc tgctggttgc gggtctgccg ctcgtcgatg
     5581 ccttgggcgt gctcatggac cagacggaga agaaaggagt gaagggtctc ctcgcggacg
     5641 tccgggaagc gatacgcggc ggggcatcgt acagcgtcgt gctcgagcgg taccccagag
     5701 atttttcttc catctatgtc catatggtgc gggccgggga agccagcgga gctctggatc
     5761 aaattttgtt ccggttggca gagttccttg agaagcaact ggctctcaag aacaaagtga
     5821 ccaatgcgat cctctatccg gcgatcatgc tggttgtcgg gaccctggtc ttgttctttc
     5881 tcgtgacgtt cgtcgttccg aagatcaccg ctgtcttcgc ccattccaaa caagccctgc
     5941 cctggccgac tattgtgctg atggaaatta gtcaattttg ctccgactac tggatggtcc
     6001 tgctcgcagg gatcctgata ggtatctggg gactacgccg gttcaaaaag acggagacgg
     6061 gccgtgtcct cgccgaccgg ctgctccttc agcttccatt gatcggtcag gtggcgcgca
     6121 tggtctcaat ttcgcggttg gccagtacgt tggccacgat gctctcgagc ggcgtgcaat
     6181 tattagaagc gctcgatgta tcgagacggg tcatgaacaa ccgcgtgctc gaacaagccg
     6241 tcgaagaggc ccggcagaac atccgtgagg gggaaacgat cgcagaaccc ctgaaacgca
     6301 gcggccagtt tcctccactg gtcacccaca tgattgccgt cggtgaaaag agcggagaaa
     6361 tggaagagat gctgcggcgc atcgggcaga tctatgacgg cgaagtggat cgcgtcatta
     6421 cgcgctttac gtcgctgctc gagcccatca tgatcgtcgt catgggcgtc gttgtgctgt
     6481 tcatcgtcgt tgcgatcttg ctccccatct ttgaaatggg acaaatggtc cggtaaccgt
     6541 tgctacccaa ggaggtatcg gtgcgcgcac agacacatac ggtccaggac ggcacagcag
     6601 tgttccgatc ggagcgcggc ttcacgttca tcgaaatcat ggtagtcgtg gcgattctcg
     6661 ccatcctcgc cgcactggtg gtgccacgga ttatgggacg aacggacgat gcgaagcgga
     6721 cggctgcgaa ggtccagatc cgtaatattg aaggtgcact ccagctgtac aaactcgaca
     6781 atggtgtcta tccgtcgacc gagcaaggtc tcaaggcctt ggtcgagaaa ccttcggtcg
     6841 gtgtcgtgcc gaaaaaatgg aaactcggtg ggtacctgcc cagtattccc gaagatccct
     6901 gggggaatcc atacaagtat ctgagcccgg cgcctgttca gcaggggcag tatgggcaaa
     6961 tcaaggccga ttatgaaatc acatcgtacg cgacggacgg cgaagccggc ggagaaggcg
     7021 tcaacgccga tatcacgaat tggaatatgg agaaggatta gaatcgtgga gccgaaagcc
//
LOCUS       DASSCQ010000104        30373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_598799, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000104 DASSCQ010000000
VERSION     DASSCQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 30373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30373
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598799"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..311)
                     /locus_tag="VFH05_07455"
     CDS             complement(84..311)
                     /locus_tag="VFH05_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678086.1"
                     /translation="MKRALMTIMAVAVLIAFSAPSFAGEEKKDKKDEKKGGHVLVYGD
                     ENKDKKEEKKGGHVDTFGEKKEDKKDEKKGK"
     gene            488..1930
                     /locus_tag="VFH05_07460"
     CDS             488..1930
                     /locus_tag="VFH05_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET6678087.1"
                     /translation="MNDIGPYSNYRLTVRLELSNKPGMFAKVASALAEEGANLGAVDL
                     ASATADRMVRDVTFDVRDEAHGERVLAKLNAIPDVTVLSASDRIFLLHLGGKIQVKSK
                     LPLTTRNVLSMVYTPGVGRVAQAIAIDKSKAYAFTTKSNTVAVVTDGSAVLGLGNLGP
                     EAALPVMEGKAMLFKELAGIDAWPICLATQDPDEIVRSVQAIAPGFGAINLEDISAPR
                     CFEIERRLKASLDIPVMHDDQHGTAVVLLAALTNALTVVGKHIEEVRVVVNGLGAAGT
                     ACCRILLAAGVSHLLGCDKEGIVLYGEAAQLRACRTDLTACLTPNRPRGSLRDALRNA
                     DVFIGLSVGNLLDAEDLDLMAADRIVFAMANPDPEVPPQLALSHCRIFATGRSDFPNQ
                     INNALAFPGIFRGALDVQASEINELMKLAAARALAETIPRAALSDDYIVPSLFDKDVV
                     PRVARAVAAAARESGTARRRSKPSDERPLA"
     gene            2036..2668
                     /locus_tag="VFH05_07465"
     CDS             2036..2668
                     /locus_tag="VFH05_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET6678088.1"
                     /translation="MPFSAAKYMKFRNGMNKRIGSLRSKTEDSMEVAVDTMMEERVDS
                     YFRIEQGLDEIIKSLLQIEEELEQICDISGAMRLESRLEFVEDRWDDLDSEIRERPRR
                     RRRRISLADMLKAAGGGGGDLSQSSGGVNNAVDAYAIMGVEFGSSLAEVTAAFRHKAK
                     RLHPDANQGDRSSEPELRRMLDAYQFLKEYLSLSNTEPMQPPKRPYSPAE"
     gene            2676..3890
                     /gene="rnd"
                     /locus_tag="VFH05_07470"
     CDS             2676..3890
                     /gene="rnd"
                     /locus_tag="VFH05_07470"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HET6678089.1"
                     /translation="MITPPEERYITDSAALEALCRELRSSRRLGLDTEFVGEDSFIPK
                     LELIQVATHDLAAVIDFPAVQAKGSLAPFWDLICDPHIEKIVHAGRQDLDLFAIHAGQ
                     IPQPFFDTQIAAAMLGFGAQVAYANLVHRIHGTKLAKAHTFTNWSARPLSSDQIAYAA
                     EDVQFLLPIHTYLQRRLQSLGRAEWVREEFSRLETSVGEKSKDPLDRYQRIRGWDNLK
                     PRQAAILRDLAAWREAEARRRNVPRGRVIRDEVLLQLARHPPRNIDELRGLRGLHGSE
                     IDRNGELLITMIRTALELPPSAWPEVPRERKPEPESIGLVELLQAVLKARAAEMEIAP
                     TLLATTADLQSLVEAKEKKRQADLPLLRGWRRQLIGDLLLQILDGSVTVSVDPTKGVL
                     RMSGDSVTTSLS"
     gene            4097..5386
                     /locus_tag="VFH05_07475"
     CDS             4097..5386
                     /locus_tag="VFH05_07475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6678090.1"
                     /translation="MVQVVLKPILAFVFPGGLLFTLALGFLRPNGLPLWCQSPVAALP
                     YLALASGLVFGWYFISARMLFTLVVLTFADRILMAFPFQKDPDPVSQIVFSLSVFLVP
                     MNILGFSLLREGRAETIRHVMAATLLLIQPLVVLWLCQPAQQDLALALVTSGLAGWST
                     SWTPIPQAALLAYVIAMTLQVSRFALSRNPMDAGAAWALGSVFLAYHCVQFGWNPTHY
                     LSTAALIVFVSLVQASYQETYRDELTGISGRLAYEETTAQLGGHFALAVLAIDQLKSY
                     AGSHGRPVVEQVLKLVSQKVEAACQGGRVFRVSGEELTLLFPHQSAMEALVALNEVRK
                     TVQSASLILRGRDRVWDNTGGTKSPGPKDRALPVTASIGVAEKSGDAASFDLVIKSAY
                     RALYEAKAGGGNAVKRGAVTRQSTTHSSGRIVTASEY"
     gene            complement(5391..5741)
                     /gene="arsC"
                     /locus_tag="VFH05_07480"
     CDS             complement(5391..5741)
                     /gene="arsC"
                     /locus_tag="VFH05_07480"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HET6678091.1"
                     /translation="MSKITIYQKPTCSTCRQALQLLKESGRPFTAINYYEQRFTASQL
                     KMLLKKAKLSPREVLRTKEDPYKQLGLANTSLTDDELIQAMVKYPDLIQRPLVEQGEK
                     ALLARPADKVRDLL"
     gene            5808..6176
                     /locus_tag="VFH05_07485"
     CDS             5808..6176
                     /locus_tag="VFH05_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678092.1"
                     /translation="MPTVGTSSSGQFSCATPTQHTLRDLRTKRKGQPVFVMGHVLERK
                     GQEATFEVFNDRLAVVKFIDGVAIGYDPYELLLPTDIDPDGTAYFEIRSCAECQQFFP
                     LTSDECESDPEPTACPECRG"
     gene            complement(6193..6576)
                     /locus_tag="VFH05_07490"
     CDS             complement(6193..6576)
                     /locus_tag="VFH05_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678093.1"
                     /translation="MKWCRALTLGVSILIGGWTLGYADDELLIFAVVSEAPKDKTRVP
                     VKASVNDVAMDTKLLASDAILNNLVWKKLEICHAMRVEGIKAPDGYRVLTVRIIDAGM
                     LPMSLQSFAGDCLIKKAIEVAPLVD"
     gene            complement(6634..6777)
                     /locus_tag="VFH05_07495"
     CDS             complement(6634..6777)
                     /locus_tag="VFH05_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678094.1"
                     /translation="MIIGVGLLTVLLILLLLSITSDPSRSSIPTKPFGTSTLSHGGPG
                     PGR"
     gene            6912..8048
                     /locus_tag="VFH05_07500"
     CDS             6912..8048
                     /locus_tag="VFH05_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET6678095.1"
                     /translation="MPHDFMPGLAGIPAAKSAVSDVDGERGVLEYRGIRVEELCERSS
                     FLETTYLLLFGRLPDEATLNKWQQDVIHHRRVKYSIIDLMKCLPEQGHPMDALQAAVA
                     ALGMYYPGRNVQDFDNNYWSAVRLVAKLPTIVAAWARLRRGDDAIPPRDELGFSDNFF
                     YMLTETIPPPVWADIFDDCLILHAEHTMNASTFTGLVTASTLADPYTVVASSIGALKG
                     PLHGGANEEVVQMLKDIGSADQARHYVEEQVRKKKKLMGFGHRVYKVKDPRATILQNL
                     CDTLFKTCGTSPLYSIALAVEQAAGETLHGKAVYPNVDFYSGIIYDKMGIDTDLFTPI
                     FAMARVSGWLAHWLEQLRENKLFRPDQIYAGEHNRPYVPVDQRR"
     gene            8227..8673
                     /locus_tag="VFH05_07505"
     CDS             8227..8673
                     /locus_tag="VFH05_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6678096.1"
                     /translation="MTLVRWDPFRELEDMSERLNRVFSRPTLRNTGKENLTVADWMPT
                     VDISETESEYLIKAELPEVRKEDVKVTVENGVLTLQGERRQEKEEKGRRFHRVERSYG
                     SFVRSFTLPESVDEGGVKAEYKDGVLALHLPKSEKVKPKAIDVKVA"
     gene            complement(8731..8970)
                     /locus_tag="VFH05_07510"
     CDS             complement(8731..8970)
                     /locus_tag="VFH05_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678097.1"
                     /translation="MDSPHLTVDAFAGYKGEKTPRSFVLEGRILSVQHIVDRWYSETH
                     SYFRIEACDGQRYVLRLDLDEDVWELIMSEPTKDP"
     gene            9051..10490
                     /locus_tag="VFH05_07515"
     CDS             9051..10490
                     /locus_tag="VFH05_07515"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET6678098.1"
                     /translation="MKNGASHKSSSPTRMERDTMGELAVPADAYYGVQTARAIENFPI
                     SPLRIPRSVIRAMGMIKRAAASVNHSLGLLDKKQADAIIEAATEVIEGGLDEEFPVDI
                     FQTGSGTSTNMNTNEVISNRATELLGGVRGSKLVHPNDHVNLGQSSNDVIPTAIHIAA
                     SETIHRQLLPALTRLHTALDRKAREFDGIVKIGRTHLQDATPVRLGQEFGGYARQIEL
                     GIGRMKRAQAALSEVALGGTAVGTGLNCHPDFSSQVVTIISRETGCPFTEAANHFEAQ
                     SSQDSLVEVSGALRTLAVSLMKIANDVRWLGSGPRCGLGEIHLPETQPGSSIMPGKVN
                     PVIAESVTMVCAQVIGNDLTVTIGGQAGNFELIVMLPVMAYNLLQSIELLAAASNNFA
                     AKCIEGITANEERCKSLIEQSLAMCTALAPEIGYEAAAKLAKEAYRSGKTVREVAKAQ
                     QVLPEARLNELLDPWRMTEPGGPVGSAGG"
     gene            10584..11936
                     /locus_tag="VFH05_07520"
     CDS             10584..11936
                     /locus_tag="VFH05_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6678099.1"
                     /translation="MSTQHKHVIIVAGAGPAGMAVAASLGKAGHEIIILNRDIKFGGL
                     AEYGIFPSKLKLRGGLRKQYWDLLQQPNVHYFGNVSIGKDKDLTVDDVRSLGASAVVF
                     SIGAQGTKAIGVEGDSAQGVFHAKDVVYHFNRLPGFGDRPFEMGKHVAVIGAGDVMVD
                     IAHWLTRYKKVDRVTAIVRRGPAERKYNPKEIRAVCANMDLEGIKNEFDRIKDRLVAV
                     GQNPDEIQKGLFDEFTKCEPAASRTKMGFRFLASPKRILVDEHNRVRGLEMEENKLEP
                     KGEDTAAVGLKQFYEFPCDSVVFAVGDKVDDTVGLPYKGGTFLTNPNKTGNDPDDALF
                     QAYDESSGKILEGLFLAGWARKASEGLVGVAKRDGDWCAEVVTRYLETKAADSPVSAV
                     LDKLQGLLKERKSRPVDITGLRALQAAEKAHKDMTDCIGEFKYVTNQEMLSLIERGGR
                     "
     gene            complement(11946..12806)
                     /locus_tag="VFH05_07525"
     CDS             complement(11946..12806)
                     /locus_tag="VFH05_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET6678100.1"
                     /translation="MKSKSIGILTKPKFPEIKATVHAVVNWLRDRKIDVILDSTSAAL
                     LNERGGVQKTQLAQKADVMLILGGDGTMLNAARLAAERGIPILGVNMGGLGFLTEVRL
                     EHLFPSLERVFANEYVLDERLMISSHIHRHGETVAQGLVLNDVVISKGTLARMIELNI
                     AIQGQFVTTLRGDGLIISTPTGSTAYSLSAGGPIISPAVRSLILSPVCPHTLTHRPLI
                     VPDDAEIDVTLTSKDEGAMATLDGQVGVALTQGDTVLLKVSEHRTRLIRFPESSYYAV
                     LREKLKWGGS"
     gene            13049..15475
                     /locus_tag="VFH05_07530"
     CDS             13049..15475
                     /locus_tag="VFH05_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6678101.1"
                     /translation="MFERFTDKGRKIIILAREEAERHQNDYLGTEHLVLAILRESDGI
                     ALMILKKMGLSTEQIRLEIERNLPGGGTTMTFGEIPFSPRVKKVIEYGVEEARLLGHN
                     HIGSEHLLLGLLREEEGIGGKILRSLGANLLTARQLTVTFLRKSAPRERDRKSNTPAL
                     DEFGRDLTQMAQEGQLDPVIGRADEIERVLQILSRRTKNNPVLIGESGVGKTAIVEGL
                     AQRIVQSEVPDNLLSRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVQAGNIIIFID
                     ELHTLVGAGAAEGSIDASNMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPI
                     YVQPPSLDETVRIIQGLRDRYEEHHGVEITEDAIVEAVKLSDRYITDRFLPDKAIDLI
                     DETGSRAKLQTYALPGELKAMEQELKKVSRDKELAISMQNFEEAVRHREEEERLRKLL
                     DESKREWKKNQEKHKPTIGKEDVAYVVSKMTGIPLFKLEEEESNKLLRMEEFLHKRVV
                     GQNEAISAVCRAIRRSRAGLKEAKKPIGSFIFLGPTGVGKTELARTLAEFLFNTEDAL
                     IRIDMSEYQEKFTSSRLFGAPPGYVGYEEGGQLTERVRRRPYSVVLFDEIEKAHPDVF
                     NLLLQVLDDGVLTDSLGRKVDFKNTVVIMTSNIGTKMIQKGVSLGFQSTDAEQDRHKK
                     EEVLGELRRQFSPEFLNRVDEIVVFHQLDKTHLTSILDILLRELNLRLVDKGVEIEVD
                     DEVKAWLIKEGYEPLYGARPMRRTIQRAIGDPLSEEMIKGRFKESRKIRVVLRDGAPT
                     FIEQEAMAEV"
     gene            15564..16667
                     /gene="tsaD"
                     /locus_tag="VFH05_07535"
     CDS             15564..16667
                     /gene="tsaD"
                     /locus_tag="VFH05_07535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET6678102.1"
                     /translation="MPRLSRNMINRQSASPWQSGPILGIETSCDETAASVLSSDGSVL
                     SNVIASQDAVHQRFGGVVPELASRAHVETIESVVGAAMEEAKLSWTDLAGLAVTQGPG
                     LAGALIVGVNYAKSLAYTLGLPLVGVSHLEGHIASAWLEDPEFPLPCVVLVVSGGHTH
                     LFLQDQHGRCRLLGCTRDDAAGEAFDKGAQMLGLGFPGGPALDRLAQTGNARAFGFPK
                     SRIKKDSLEFSFSGLKTALLYTLHSMEERQQVKLKADLAAGYQEAIVTVLIDKAFMAL
                     KQTGVRALAVVGGVSANSRLRCLLQERALATDTRISLPPLRFCTDNAAMVAAAGRQAL
                     IAGRRMPLDAEPWSTLEPTSESPFAITPEASHS"
     gene            16690..18342
                     /gene="hflX"
                     /locus_tag="VFH05_07540"
     CDS             16690..18342
                     /gene="hflX"
                     /locus_tag="VFH05_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET6678103.1"
                     /translation="MRPSQVTAIERLYRRRVPHDKVLSPELGRTMCQISREIRRPIAV
                     LLSRRGQVQEIIVGTDLTLSATTLSKFRVGARSLRGLRVIRTQLHDQPLSQESLTDLA
                     YLRLDLFGALSVTPEGNPGNLYLAHLLPPNGTGQVCNILKHTPFHHATMAFDRFIEDL
                     EADMQRAQGTHAVGHERESAMLVSASSRSRVEQEDRLLELAELASSAGVTVVDRMAQR
                     TQDGHQRYVLGSGKLKDVLIQTLHRGADMVIFDQTLTPAQSRAISEMTDIKVIDRTQL
                     ILDIFARRAHTREGKVQVELAQLRYLLPRLSGKGQELSRLGGGIGSRGPGETKLETDR
                     RRIRDRITHLERELDQFGRHQDQRRARRTRHGLPVVSLVGYTNAGKSTLLNVLTHSHV
                     STVDRVFETLDTTSRRLRFPQDREVIITDTVGFIRDLPEELLGAFRTTLEELRQADLL
                     VHVVDAGAADVDIQISAVVKILQELNLDMLRRTLVFNKCDRIPPAEADRLCRRYQAIG
                     VSALHPDTLRPLLDHLDRELAGVALPQPTSQVDQAAALASRL"
     gene            18368..19030
                     /gene="nth"
                     /locus_tag="VFH05_07545"
     CDS             18368..19030
                     /gene="nth"
                     /locus_tag="VFH05_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET6678104.1"
                     /translation="MRRPPTSPAANRKRLQRIAQALEAAMPEAKIELSHRSPWELLVA
                     TILSAQCTDQRVNQVTPALFTRYKTPSELAKARLPDVEAIIRSTGFFKSKAKNIVACS
                     QVLTERFGQRVPERMDDLVTLPGVGRKTANVLLGQAFGQPAIVVDTHVKRVANRLDVT
                     GSEDPVQIEQDLQQLLPSSQWTSVSQRLLLHGRYICLARRPLCGRCSIYDDCYAREKL
                     PR"
     gene            19027..19905
                     /locus_tag="VFH05_07550"
     CDS             19027..19905
                     /locus_tag="VFH05_07550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET6678105.1"
                     /translation="MIQSMTGFGRKQAPWQEGTITVEVRSVNHRFLEMSCRLPRTLAM
                     LEESFKKAIQQRCTRGRVDLTVTIQGTKGRSGTVALDRALAKQYHHALRTLKASLKLS
                     GPIDVALIAGLRDVVSVSDQPPEEPELARMVQQLANQALDDLHHMRKQEGLALAKDMR
                     KRLETMKDHRSAVASRSPIVVQESFERMKERIAKLLGTEPPDLPRLHQELAVYADRGD
                     ITEEIVRLDSHMLQFEQQLNRAESVGKTLEFLLQEMGREVNTIGSKANDAAIAGHVIH
                     MKAELERIREQVQNVE"
     gene            19926..20603
                     /gene="gmk"
                     /locus_tag="VFH05_07555"
     CDS             19926..20603
                     /gene="gmk"
                     /locus_tag="VFH05_07555"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET6678106.1"
                     /translation="MPSGPVRSSTERRGILFIISAPSGTGKTTLCKQLVANIPGLWHS
                     VSYTTRTPRPGEEHGREYYFTDDTTFQAMIDRHEFVEWARVYGHLYGTPRRMVADKIE
                     QGIDVLLEIDVQGALQVKKKFDDAVSIFILPPSMTVLRQRLLNRASDSSEEIQRRLQK
                     VKEEVWSYREYAYIVRNDDLTRSLQDLESIFLGERLKTKRLNMAWIENNFILDDEAKP
                     GDRKDLP"
     gene            20623..20982
                     /locus_tag="VFH05_07560"
     CDS             20623..20982
                     /locus_tag="VFH05_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET6678107.1"
                     /translation="MIDMLSLLPQYTPDQFDSRHRLVIVAAQRAKHIVQGSRPFGMSR
                     FTKETTIALDEVLRGEAKYLIGKEARDAMKEAKRGKEGETERMAMMTGEDAREIKKEL
                     SVYVDDSPKPVAGEPEE"
     gene            20986..22242
                     /gene="coaBC"
                     /locus_tag="VFH05_07565"
     CDS             20986..22242
                     /gene="coaBC"
                     /locus_tag="VFH05_07565"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET6678108.1"
                     /translation="MEDHVRNPSLTGARIVLGVTGSIAAYKAVTLLRALVREGAVIDV
                     AMTRSATKFVTPLTFEVLSGRPVTADLFQAHQEMKHLSLPEHADVILVAPATANFLAK
                     AALGLGDDLLSTMLLTGRCPLICAPAMDGGMWSHPTVVAHVEALRARGALIVDPEEGP
                     LASGRIGQGRMAGEDRILDAVRAVLTPKRDWQGQRILLSAGPTQEPIDPVRYLSNRSS
                     GKMGYAIAEAARKRGAEVILVSGPTSLPAPSGVEVIPVETAEEMLKQLSSRLSWSTVV
                     VMAAAVADFRPRQVAPQKLKKQGRRSATLELEQTTDILSTISGQRTTQLVIGFAAETH
                     DLLPHARAKLASKGLDLIVANDVTRPGAGFGSDFNAATLIARDGHTTDIPLKSKRELA
                     DDILDAVRRLLVDSPTRRHGDLRKRR"
     gene            22245..23003
                     /locus_tag="VFH05_07570"
     CDS             22245..23003
                     /locus_tag="VFH05_07570"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678109.1"
                     /translation="MPPQKNIASALEIDRLATQLATNPRSKAFLPLAEEYCKAGMWEE
                     AVGVLEDGLKYYPGFITAMVVLGRAYDQLNQPTKARAVLEGAVKLSPENLRAHRTLIK
                     IYAAQGLTEEALKSCHVILAMNPRDEEVLSLQASLGATESEKGQGAPSPKSVEVTGQV
                     TVTPGTNTASGPPPALESVPTSIPDIPTGKLPSPLDDQKPPQDVAPSSDSDVVTTESQ
                     QTSHADNVARLESWLRSLERQRRDRSTRDTHSGS"
     gene            23115..23609
                     /gene="aroQ"
                     /locus_tag="VFH05_07575"
     CDS             23115..23609
                     /gene="aroQ"
                     /locus_tag="VFH05_07575"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET6678110.1"
                     /translation="MLRILVLHGPNLNLLGTREQSIYGTQSLENIDSALTTLAEHLGL
                     HIDIHQSNSEGDLVNWIQEGGSGYHGIIINPAAYTHTSVAIRDAISAVGLPTVEVHLS
                     NIHQREPFRHHSHVAGVALGQISGLGPSGYLLALRGLHDHLAGTKKLKNSPTASRRQK
                     RASR"
     gene            23648..24208
                     /gene="efp"
                     /locus_tag="VFH05_07580"
     CDS             23648..24208
                     /gene="efp"
                     /locus_tag="VFH05_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET6678111.1"
                     /translation="MISTTDFRGGVRLLVDGEPYYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGERFEEPDLEEREMQFLYATGDEYTFMDTETYEQLTFEKQQLGDNA
                     DLLKENMNVKILIYEHRPIDVELPIFIELKVVDAEPGVRGDTASGGTKPAVVETGATI
                     KVPLYLEVGTVIKIDTRTRAYVERVR"
     gene            24253..24765
                     /gene="accB"
                     /locus_tag="VFH05_07585"
     CDS             24253..24765
                     /gene="accB"
                     /locus_tag="VFH05_07585"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET6678112.1"
                     /translation="MLPGSFAGATSAQDAAAQQRQIQDLIDLLKRNHLTELEIERAGV
                     RIRVRHEAAVKTVSAAVSEAGQGTGQTVSPHTSPPNELAEDTAGHITITSPIVGTFYR
                     SPSPDADPYVEEGDFVKRGQVLCIVEAMKLMNEIESEVDGRITKILVESTKPVEYGQA
                     LFLIDPKATP"
     gene            24835..26175
                     /gene="accC"
                     /locus_tag="VFH05_07590"
     CDS             24835..26175
                     /gene="accC"
                     /locus_tag="VFH05_07590"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6678113.1"
                     /translation="MFKKVLVANRGEIALRVIRACKELGIKTVAIHSEADAAGMHVRA
                     ADERICVGPAESALSYRNIPNVLSAAEITGADAIHPGYGFLSENAHFAEVCESIGVKF
                     IGPTSENIALMGDKAKAREIVARRGLPVTPGSPGELKSEQEAREAAAKIGYPVIIKAA
                     AGGGGRGMRVVNSTDEVARAFQAAQAEAKSTFGNDSVYLERYFLEPRHIEVQIAADSR
                     GHVVYLGERDCSIQRRHQKLVEETPSPAVDEKLRREIGRVAVEAVKAVHYRNLGTVEF
                     LLDKDHNFFFMEMNTRIQVEHPITEMVTGIDLIKEQITLANGQPLSFRQPDIRLTGHS
                     LECRINAEDPEKFTPSPGVISRYCAPGGFGVRVDSAMESNAVVVPHYDSMIAKVITHG
                     RDRLEAMARMRRALDEFVIEGIKTTIPLHKRILNDPDFQKGHVSTTYLERFLAS"
     gene            complement(26189..26608)
                     /locus_tag="VFH05_07595"
     CDS             complement(26189..26608)
                     /locus_tag="VFH05_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET6678114.1"
                     /translation="MKQTPGNSPLDMSCPLPEQSPRPSVMAVVLFLIASMSGCESREQ
                     TVRIVAEDFRFTPSEVHVSSAYPIRLLVVNEGRERHEFKTALLAHQIGMSPGPSSSLP
                     VPPNQKVEALVRPIPGVYLFYCARRGHAGMSGTIMVE"
     gene            26633..27271
                     /gene="thiE"
                     /locus_tag="VFH05_07600"
     CDS             26633..27271
                     /gene="thiE"
                     /locus_tag="VFH05_07600"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6678115.1"
                     /translation="MSRNQRTRTLSGLYVILDSSTRPNRPLIDVLNGAAIAGVSIFQY
                     RNKTASMKDAFREALPLRNRARELEVLFLVNDRCDLALALDADGVHVGQDDLPVAQAR
                     RILGPEAVIGLSTHSDRQVEAADRLQPDYIGFGPIFAPGSKTDHDPIVGIDALKRIRV
                     LTRLPIFAIGGIRLGQVRAVIEAGADGIAVISALASAPDIEVAVSALLEQMS"
     gene            complement(27243..28001)
                     /gene="bioD"
                     /locus_tag="VFH05_07605"
     CDS             complement(27243..28001)
                     /gene="bioD"
                     /locus_tag="VFH05_07605"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET6678116.1"
                     /translation="MIPRRVTGGAGVFVTGTDTGVGKTLVTAALAIRLRRLGRTVGIM
                     KPIETGVTSSRLIQSDAARLRAAIDSEETLGAICPYQFEQPVAPLGAAHIERRTIDPR
                     VIRQVYRLLANRYQSVVVEGIGGVRVPITADADLMDVMRGLKLPAIVVGRAGLGGINH
                     ALLTIDALHRRRIPVVALILNRTHPVRSSVTRLQEKTTLDALRKQAGVPVLGPLPYVP
                     GLMRRFRQSASRFANMAAITMLAKLVRTSARGGR"
     gene            complement(27998..28705)
                     /locus_tag="VFH05_07610"
     CDS             complement(27998..28705)
                     /locus_tag="VFH05_07610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6678117.1"
                     /translation="MTGASGGIGQAISRGFGKAGWCVGIHYHREKLAAESTLHDVQKA
                     GGSGQLYEADVRDAQAVHHLIAAFIRANPRPVCFVCCAGVGRGSLLLKLSTEEWADVM
                     ATNLTGTFHSLRAMAPALLAQGGGSIVVLGSHAGSQGSTGQAAYAASKAGLVGLVKTA
                     AVEWGPDNICINLVLPGWQHTRLTTEAMSRGQQWRDHALGRPPALEEVVRTVMHLVEL
                     KDVSGQVWNCDSRSLFA"
     gene            28921..29784
                     /locus_tag="VFH05_07615"
     CDS             28921..29784
                     /locus_tag="VFH05_07615"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678118.1"
                     /translation="MRFCSLTVLCLALSWSVSLAAEELTGIVQSSDYFPDTIGSHWEY
                     HGQITEGPLQTIEHKFFTNVSTVVGTKPLRGMTVTVFHDTNPGNHGPSDSFYRRDVVG
                     IVYHGSDPGTPLEKQLIPYQIVRFPIQIASTIQQFDKKGLDFGTDMDRDGVNEKVDAH
                     GTSTVAGLESITVPAGTFKDAVKVEAYMRMKIHLSGASRTVTGTDVMTAWFAKGVGLI
                     KYVERQELSPLEDRGVITEIMEELDSYEVKSPNASLGRSETPAERLFAHDSRHHELQQ
                     VIFPSSFRPHP"
     gene            complement(29677..>30373)
                     /locus_tag="VFH05_07620"
     CDS             complement(29677..>30373)
                     /locus_tag="VFH05_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6678119.1"
                     /translation="IIVDETADLDDAVAGVVASSTSYSGQKCSACSRVIVHAAVYDLF
                     LSRLRDAIASLRVGNPEEPGTQMGPVIDERAQASILEYIKIGSQEARVVVEGRVPQPG
                     WYVAPTVFADVKPTARIAQEEIFGPVLAVMRAESFDEALAVANGTDYALTGGVYSRSP
                     ANLDLARLRFDVGNLYLNRPITGALVNRQPFGGHRLSGVGAKAGGEDYLLQFVVTRIV
                     SEQTLRRGFAPAE"
BASE COUNT         6794 a         8815 c         8645 g         6119 t
ORIGIN      
        1 cccaaaaaaa aggtctatgg ccgtaagacc atagaccttg gggaatactc gaggcccgac
       61 aaacgtgaag gagccgtagc ggcttacttg cccttcttct cgtccttctt gtcttctttc
      121 ttctctccga aggtgtccac gtggccgccc ttcttctctt ctttcttgtc cttgttctca
      181 tcgccgtaca cgagaacgtg gccgcccttc ttttcgtcct tcttatcctt cttctcctcg
      241 ccggcgaacg aaggagcgct gaatgcaatc aacaccgcca ctgccataat agtcattaac
      301 gcgcgcttca taggtagccc cccttagact gtgcatttgc ctgaccatca gacaaacggg
      361 cgcataccgt agccaatgaa ttagggattt gtcaatgacc acgaatacca agacaattgc
      421 gtgcatcaat cgcccggtcg cgtcctctgt gataaggtta agagacagta tttcggctca
      481 tcgtcccatg aatgacatcg gcccgtactc aaactatcgc ctcaccgttc gactggaact
      541 atcgaataaa ccggggatgt ttgccaaggt agcctcggct ctcgccgaag aaggggccaa
      601 cctcggcgct gtggatctgg cgtcggcaac cgccgaccgg atggtcaggg acgtcacctt
      661 tgatgtccgg gatgaagccc atggcgagcg ggttctggct aaattgaatg ccattccaga
      721 cgtcaccgtg ctctcggcct cggatcgaat ttttctcctc cacctcggag ggaagatcca
      781 ggtgaagagc aagctgccgc tgactacacg taacgtgctg tcgatggttt atacgcctgg
      841 cgtgggccgt gtcgcccaag cgatcgccat cgataaatcc aaagcgtatg ccttcacgac
      901 aaagagtaac actgtggcgg tggtgacaga cggatcagcg gtgttaggcc tggggaatct
      961 cggaccggaa gcggcccttc cggtcatgga aggcaaggcg atgctattca aggaattggc
     1021 cggaatcgat gcctggccta tttgcctggc gactcaagat ccggatgaga ttgtgcgctc
     1081 cgtccaagcg attgcccccg gattcggggc catcaacctc gaggatatca gcgcacctcg
     1141 ctgttttgag atcgaacgtc gactcaaggc gtcactggac attccggtca tgcatgacga
     1201 ccagcacggg accgcggtgg tcctgctcgc cgcattgacc aatgccttga ccgtagtagg
     1261 taagcatatc gaagaggttc gtgtcgtggt caatgggctt ggcgccgcgg gtactgcctg
     1321 ctgccggata ctgcttgcag ccggggtgtc ccatctcctc ggctgcgata aggaaggaat
     1381 cgttctgtac ggagaggccg cccaactgag ggcctgccga accgatctga cggcatgcct
     1441 gacgcccaac cggccacgcg gcagcttgcg cgacgcatta cgcaatgccg atgtattcat
     1501 cggactttcc gtgggcaacc tgttggacgc ggaggacctt gacctgatgg cggcggatag
     1561 aatcgtcttc gccatggcca atcccgaccc cgaagtgccc cctcaactgg cgctttccca
     1621 ctgccgcatc ttcgcgaccg gccgctcgga tttccccaac cagatcaaca acgcgttggc
     1681 tttcccaggt attttccgtg gcgcgctcga tgtgcaggcc agcgagatca acgaactcat
     1741 gaaacttgcc gcagcccggg ctttagccga aacgattcca cgggccgcac tcagcgatga
     1801 ctacatcgtg cccagcctgt tcgacaaaga tgtcgttccc cgggtggcgc gagcagttgc
     1861 cgcagctgcg cgtgagagtg ggacagcacg tcgaagatcg aagccttcgg atgaacggcc
     1921 cctggcttga cccccactga gcgggcgcgt gtttctcctg agaatgcacg tgtcctcact
     1981 tcagcgcccc tgttctcctg tgctacaatg cccgcattcc cggagcgtct acgccatgcc
     2041 cttctccgcc gccaagtaca tgaagtttcg caacggcatg aacaagcgga tcggctcctt
     2101 gcgttcaaag acggaagaca gtatggaagt tgccgtggac accatgatgg aagagcgggt
     2161 agacagctac ttccggattg agcagggcct ggacgagatc atcaagtctt tgttgcagat
     2221 agaagaagag ctggaacaaa tctgcgatat atcaggcgcc atgcgcctcg aatcaaggct
     2281 tgagttcgtg gaagatcgtt gggatgacct cgacagcgaa attcgcgaac ggcccaggcg
     2341 ccggaggcga cgcataagcc tcgctgatat gctgaaggcc gcgggcggag gcggcggtga
     2401 tctctcccaa agttcaggag gtgtcaataa cgccgtagac gcctatgcca taatgggagt
     2461 agagttcggc agttctttgg ccgaggtgac tgcggcattc aggcacaagg cgaagcggtt
     2521 gcatcccgat gccaatcagg gcgatcgcag ctctgagccg gaactgcggc ggatgttgga
     2581 tgcctatcag tttttgaagg aatatctgag cctcagtaac acggaaccga tgcagccgcc
     2641 gaagcgcccc tacagcccag ccgagtaatg ccacagtgat cacccctccc gaggaacggt
     2701 acattaccga cagcgcggca ctcgaggctc tctgcaggga gttacggtcc agtcgccggt
     2761 tgggactgga tacagaattc gtcggcgaag acagcttcat ccccaagctg gaattaatcc
     2821 aagtcgccac gcatgacctt gcggccgtca tcgactttcc cgccgtccaa gccaagggat
     2881 cgcttgcgcc gttctgggac ctgatctgcg atccacatat cgaaaagatc gtgcatgcag
     2941 gtcgccaaga tcttgacctc tttgcgattc acgctgggca gattcctcag ccgttttttg
     3001 acacgcaaat cgcggcggct atgctcggct tcggtgcgca agtcgcttat gcaaatttgg
     3061 ttcatcggat tcatgggacg aagttggcaa aggctcatac gtttaccaac tggagtgctc
     3121 gaccgctttc ctctgatcaa atcgcctatg ccgccgaaga tgtgcagttt ctgctgccta
     3181 ttcataccta cctgcagcgg cggctacagt cgctcggccg ggccgaatgg gtccgagagg
     3241 aattttctcg cctagagaca agcgtagggg agaagagtaa agatccgctc gatcgctacc
     3301 aacgcatcag gggatgggat aacctgaagc ccagacaggc tgccatccta cgagacctgg
     3361 ccgcatggcg cgaagccgaa gcccgccggc gtaacgtacc caggggccgt gtgatccgtg
     3421 acgaagtgct gttgcaattg gcgcggcatc cgccgcgcaa tattgatgaa ctccgtgggc
     3481 tccggggctt gcacggatct gaaatcgacc ggaacggtga gctgttgatc accatgatca
     3541 gaacggcgct tgaacttcca ccttctgcct ggcccgaggt gccccgagag cggaaacctg
     3601 aaccggagtc cattggattg gtggaacttc tccaagcggt gctgaaagca cgagcggccg
     3661 agatggaaat tgctcccaca ttactcgcaa cgacagccga cctccagtcg ctcgttgaag
     3721 ccaaggaaaa gaagagacag gcagaccttc ccctgttgcg aggatggcga cgccagttaa
     3781 tcggcgatct acttttacag atcctcgatg gcagcgtgac ggtttccgta gacccaacaa
     3841 agggagttct gcggatgtct ggagatagtg tgaccaccag tttgtcataa gctggccgca
     3901 tccttcgtcg ttcgtcttca ccttcgcact gttcaaaaac tgcctgagcc catccaccac
     3961 gtgcatcgcg gtcccggccc aggggcacgc acccccagtg atttcggcgt cttgcgcaat
     4021 cctccactcg ctcggctgca gcatcaactg gtacagaact ggcaccttcg agctcgacct
     4081 catccgcgag ccgctcatgg tacaagtcgt actcaaaccg atcctcgcct ttgtatttcc
     4141 cggagggctg ctctttaccc tcgcactggg ctttctccgc cccaacggac tcccattgtg
     4201 gtgtcagagt ccggttgccg cattgcccta tctggcgctg gcatcaggcc tggtgttcgg
     4261 atggtacttt atcagcgcga gaatgctctt cacgttggtc gtcctcacat tcgccgatcg
     4321 gatactcatg gcatttcctt ttcaaaaaga tcccgacccc gtgagtcaga tcgtgttttc
     4381 cctatccgtg ttcttggtgc cgatgaacat cttgggattc tctctcttgc gagaaggacg
     4441 agctgagacc atccggcacg tgatggccgc gacgctgctt ctcattcagc cccttgtcgt
     4501 gctgtggctg tgtcagccgg cacaacagga tctggccctg gcccttgtca catccggact
     4561 tgcagggtgg tcgacgagtt ggacaccgat tccacaggcg gcgctcctgg cctatgttat
     4621 cgccatgacg ctacaggtga gccgctttgc gttgagtcgg aatcctatgg acgcgggagc
     4681 cgcatgggca ttgggtagcg tgtttctcgc atatcactgc gtgcaattcg gatggaaccc
     4741 gacccattac ttatccaccg cagcgctgat cgtctttgtg tcgcttgtgc aagcttcgta
     4801 tcaggaaacg taccgggacg aactgacggg gatctcggga cgtctggcgt atgaagaaac
     4861 caccgcccaa cttggcggcc actttgccct cgctgtgctc gcgatcgatc agttgaagtc
     4921 ctatgcagga agccatggac ggccggttgt ggaacaggtt ctgaaactcg tgtcccaaaa
     4981 agtggaggct gcctgccagg gagggcgcgt gttccgtgtg agcggggaag aattgacctt
     5041 gcttttcccg caccagtccg cgatggaagc tctggtcgcg ctgaatgagg tgcgaaaaac
     5101 tgtccagtcc gcctctctga ttcttcgggg ccgcgaccgt gtgtgggaca atactggcgg
     5161 aacaaaatcg cctggaccga aggatcgagc gctcccggtg accgccagca tcggagtggc
     5221 tgaaaaatcg ggagacgcgg cttcgttcga cctggtcatc aagagtgcct accgcgccct
     5281 ctatgaggcg aaagccggag ggggaaatgc ggtaaagcgg ggcgcagtga cgcgccaatc
     5341 caccacacac tcgtccggcc ggattgtcac ggcgagcgag tactaatgcc tcacaagaga
     5401 tccctcacct tgtctgcagg acgggctaac aatgccttct caccttgctc gaccagcgga
     5461 cgttgaatca aatccggata cttcaccatc gcctggatga gctcatcatc cgtcagggat
     5521 gtgttggcga gtccgagctg cttatagggg tcttctttcg ttcttaggac ctccctcggc
     5581 gagagtttgg cttttttcaa cagcatcttg agttgtgagg cagtgaaccg ctgttcgtag
     5641 taattgatcg ctgtgaatgg tctgccactt tccttgagta attggagggc ctgccggcag
     5701 gtcgagcagg tgggtttttg gtaaatggtg atttttgaca tacgtcctcc acactcgttg
     5761 cttgacgcct ttttttcgct tgtggtacat gcttctcagc acccaccatg ccgacggtcg
     5821 gaaccagttc cagcggtcag ttctcctgcg caaccccgac gcagcatacg ttgcgcgatc
     5881 ttcgcaccaa gcggaagggc cagccggtgt tcgtgatggg tcatgtactc gaacggaaag
     5941 gacaggaagc taccttcgag gtgttcaatg accgtctggc cgtggtgaaa ttcatcgacg
     6001 gggtggcgat tggatacgac ccctacgaac tcctccttcc caccgatatc gatccggatg
     6061 gcaccgcgta ctttgagatc cgttcttgcg ctgaatgtca gcaattcttt cctctaactt
     6121 ccgacgaatg cgagtccgac cccgaaccga ccgcttgccc ggaatgccgc ggctaagcac
     6181 ggcagcccct ggtcaatcaa ccaaaggcgc aacttcaatc gctttcttaa tcaggcagtc
     6241 gcccgcgaac gattgcagag acatgggcag catgcccgca tcgatgatgc ggacggtgag
     6301 aactcggtag ccgtcaggcg ctttgatgcc ctcgacacgc atcgcatgac agatttccaa
     6361 ttttttccac acaagattgt tgaggatggc gtctgacgcg agcagcttgg tatccatggc
     6421 aacatcattc accgaagcct tgacgggcac gcgtgtctta tccttcggtg cttcactgac
     6481 gaccgcaaaa atcagcaact cgtcgtcggc atatccgagt gtccatcctc cgatgaggat
     6541 actgactccc aacgtcaagg cccgacacca cttcatgcct gtcctcgcta tctgtccagg
     6601 ggcaagccgg cgcgtgcacc aatgtggcga cggttaacgc cccggcccag gtcctccatg
     6661 actgagcgtg ctggttccga agggcttggt cggaatcgac gaccgtgaag gatcactggt
     6721 gatcgaaagc aagagcagaa taagcagaac cgtcaggagc ccgacgccga tgatcatggt
     6781 aaggagtcgt ttgctcggcg gggtccaaac catcgcagca gtatgccaat cgactcgagt
     6841 ctttgcaagg tcgtgccgta tgacttgcat cgtcgtccca agtggggcac agtgaaacat
     6901 ggagaagttc tatgccacat gacttcatgc ccggcctcgc cggaattccc gccgccaaat
     6961 ctgccgtcag cgacgtcgat ggcgaacgag gagtgctcga gtatcgcggc atccgggtgg
     7021 aagagctttg cgaacggtcc tcatttctgg agacgacgta cctccttctt ttcggccgcc
     7081 tgccagatga ggcgaccctc aacaaatggc aacaggacgt catccatcat cggcgggtca
     7141 aatactccat catcgacctc atgaaatgcc tgcccgaaca gggccatccc atggatgcac
     7201 tgcaagcggc tgtggccgca ctcgggatgt actatccggg ccggaatgtc caagattttg
     7261 acaataacta ttggtcggcg gtgcgcttgg tcgccaagct gccaacgatc gtggcggcat
     7321 gggcgcgact tcggcgcgga gacgatgcca ttccgccccg agatgaactg ggcttcagcg
     7381 ataacttctt ctacatgctg acagaaacca taccgcctcc tgtgtgggca gacatcttcg
     7441 atgactgtct gatcttgcat gccgagcaca cgatgaatgc ctctacattt acaggactcg
     7501 tgaccgcctc gacgttggca gacccctata cggttgtcgc ctcttcaatc ggcgcgctta
     7561 aagggccact ccatggtgga gcgaacgaag aagtggttca gatgctgaag gacatcggat
     7621 cggccgatca ggctcgtcac tatgtcgagg aacaggtgcg caagaaaaag aagctcatgg
     7681 gcttcggaca ccgcgtctat aaagtgaaag atccccgggc gaccatcctg caaaatctct
     7741 gcgacacgct cttcaagacc tgcgggacct caccgctcta cagcatcgct ttggcggtcg
     7801 agcaggccgc gggcgagacg ttgcatggaa aagcggtcta tccgaatgtc gatttctatt
     7861 ccggcatcat ctacgacaag atgggaatcg acacggactt gttcacgccc attttcgcga
     7921 tggcccgggt gtcaggatgg ctcgcccatt ggctggaaca gttgcgggaa aacaagttgt
     7981 ttcgccccga tcaaatctac gccggagagc ataaccgccc ctatgtgccc gtggaccagc
     8041 gtcgataact gggcggtcca ccccatcacc aggccagctc tagctttgtt agttcgccgc
     8101 gaaatcgtta gtcccgctgc ctactgcctc ttgactctgc cagcgcccgt cttatctgtt
     8161 cgctaggcga aaatcggcgc agcgccaaag actgaacaaa aaaagcgaca gtcaaggagg
     8221 tgaactatga cactcgtacg gtgggatccc tttcgtgaat tggaagatat gtctgagcga
     8281 ttgaaccgcg tgttctcgcg gccgacgttg aggaataccg gtaaagaaaa tctcacagtt
     8341 gccgactgga tgccgacggt cgacatcagc gaaaccgaat cagagtacct gatcaaagcc
     8401 gagctgcccg aagtgagaaa agaagacgtg aaggtgacgg tagagaatgg ggtgctgacc
     8461 ttgcaggggg aacgccgaca ggaaaaagaa gagaagggga ggcggtttca tcgggtcgag
     8521 cgctcgtacg gaagctttgt ccgaagcttc acacttccgg aatccgtgga cgaaggcggc
     8581 gtcaaggctg agtataaaga cggcgtcttg gccttgcatc tgccaaagtc ggaaaaggtc
     8641 aaacccaaag cgatcgatgt aaaggtggcc taaagattca tacgaaggcc cgctccggcg
     8701 ggccttcgtc tttttgtgta tgtccgcccc tcacgggtct ttggtcggtt ccgacatgat
     8761 caactcccac acgtcctcgt cgagatccaa ccgcaataca tagcgctgcc cgtcacaagc
     8821 ctcgatccta aaatagctgt gcgtctcgct gtaccagcga tccacgatgt gttgaaccga
     8881 tagaatccgg ccttcgagca cgaaagaacg cggcgtcttt tcgcctttat atccggcaaa
     8941 agcgtcaacg gtgagatgag ggctatccat cactgactct tgtagaagta ggtggcctga
     9001 gtttatcatg gcgcccgcag cgtgaaagcc ttccaagcat ggagcaacgc atgaagaacg
     9061 gagcctctca caaatcgtca tcgccaacaa gaatggagcg cgacacgatg ggcgaattgg
     9121 ccgttccggc tgacgcctat tatggcgtac agacggcgcg agcgatcgaa aactttccca
     9181 tcagccccct gcgcatccca cgatctgtca tcagggcgat ggggatgatc aagcgggctg
     9241 ccgcttctgt gaaccattcg ctggggctgc tcgacaaaaa acaggcagac gccatcatcg
     9301 aggcggcaac cgaagtcata gagggggggc tcgacgaaga gtttcctgtc gatatttttc
     9361 agaccggatc aggcacctcg acgaacatga ataccaatga agtcatttcc aaccgtgcaa
     9421 ctgaattgct cggtggcgtc cggggcagca agctcgtcca tccaaacgat cacgtcaacc
     9481 tcggccagtc cagcaacgac gtcatcccca cagccattca cattgctgcc tctgaaacga
     9541 tccatcgtca actgttgccg gcgttgacca ggctccatac cgctctcgac cggaaggcta
     9601 gagagttcga tggaattgtc aagatcggca gaacccattt gcaggacgcg acccctgtgc
     9661 gtcttggaca ggaattcggc gggtacgccc gacagatcga actgggcatt ggacggatga
     9721 agcgtgccca agcggccctg agcgaagtgg ccctcggggg gaccgccgtc ggcaccggat
     9781 tgaattgtca tcctgatttc tcctcgcagg tggtcactat tatatcccgt gaaacaggct
     9841 gtcccttcac agaagcggcg aatcatttcg aagcccaatc gtcacaggac tcactggtcg
     9901 aggtgagcgg ggcattgcga acattggcag tgagtctcat gaaaatcgcg aatgatgttc
     9961 gatggctcgg atccggaccg cgatgtggct tgggagaaat ccacttaccc gaaacacaac
    10021 caggatcctc catcatgccc ggaaaggtca atcctgtgat tgccgaatcg gtaaccatgg
    10081 tatgcgccca agtcatcggg aacgacctga cggtcaccat cggcggacaa gccgggaatt
    10141 tcgagttaat cgtgatgctg cccgtgatgg cctacaacct cctacagtcc atcgaactgc
    10201 ttgcggctgc ctccaacaat ttcgccgcca aatgcatcga gggtatcacc gcgaatgaag
    10261 aacgctgtaa aagtctcatc gaacaaagcc ttgccatgtg taccgccttg gctcctgaaa
    10321 tcggctatga ggcggcagcg aagttggcga aggaggcgta ccgatctggc aagacggtgc
    10381 gggaagtcgc caaagcgcaa caggtgttgc ccgaggcacg gctgaacgaa ctgctggacc
    10441 catggcggat gaccgaaccc ggtgggccgg tgggaagcgc aggcgggtga aacgaccagg
    10501 gccggttttg acagtgtcgg aaccgctatg ctaaagtccg cgtaactgtt agctttcttg
    10561 tcatttttca caatcaggta gatatgagca cacagcacaa gcatgtgatc attgtcgccg
    10621 gtgcaggtcc ggccggtatg gccgtcgctg cctcgctcgg gaaagccggg cacgaaatta
    10681 tcatcctgaa tcgcgatatc aaattcggtg ggcttgccga gtatgggatc ttccccagca
    10741 aactgaaact tcgcgggggc ctgcggaaac agtactggga tctcctgcag cagccgaacg
    10801 tccattattt cggcaacgtg tcgatcggaa aagacaaaga tctgactgtc gatgacgtcc
    10861 gctcactcgg cgccagcgca gtagtgtttt caatcggcgc acaggggact aaagccatcg
    10921 gcgtagaagg ggattctgcg cagggcgtct tccacgccaa agacgtcgtt taccatttca
    10981 accggctccc cggctttggt gatcgcccgt tcgaaatggg caagcacgtc gcagtcatcg
    11041 gtgccgggga tgtcatggtg gacatcgcac actggctgac tcggtataaa aaagtcgacc
    11101 gcgtaaccgc gatcgtacgg cggggtccag cggaacggaa atacaatccc aaagaaatcc
    11161 gcgcggtttg cgccaatatg gatctggaag gcatcaagaa cgagttcgac cggatcaagg
    11221 atcgactggt ggccgtcggc caaaatccgg acgagataca aaaagggctg tttgatgagt
    11281 tcacgaaatg tgagcctgcg gcgagtcgga cgaaaatggg cttcagattc ttggcttcac
    11341 ccaagcgcat ccttgtcgac gaacacaatc gtgtccgtgg tcttgaaatg gaagagaaca
    11401 aactagagcc caaaggcgag gacacggccg ccgtgggact caaacagttt tacgagttcc
    11461 cttgtgactc ggttgtgttc gctgtcggcg acaaggtcga cgacacggta ggattgccct
    11521 ataaaggggg tacttttctg accaatccca acaagactgg caacgaccct gatgatgcgc
    11581 tctttcaagc ctatgacgaa tcatccggca aaatcttgga aggactcttc cttgccggtt
    11641 gggcgagaaa ggcgagtgaa ggcttggtcg gggttgccaa aagggatgga gattggtgcg
    11701 ctgaagtcgt gactcgttat cttgaaacga aagccgcaga cagcccagtg agcgctgtct
    11761 tggataaact acaagggctg ctcaaggaac ggaagagccg gcccgtcgac atcacaggtc
    11821 ttcgagccct ccaagcagcc gagaaagcgc acaaggacat gacggactgc attggagaat
    11881 tcaagtacgt gacgaaccag gaaatgctca gcctgatcga gcgcggcggc aggtagccag
    11941 accattcaac ttccgcccca tttcaacttc tcacgcagga ctgcgtagta gctgctttcc
    12001 ggaaagcgaa tcaaccgtgt gcggtgttca gagaccttca agagcaccgt atccccttgg
    12061 gtcagtgcaa cacctacctg cccatcaagc gtggccattg cgccttcatc cttgctcgtt
    12121 aacgtcacgt caatctcggc gtcatctgga acgataagag gccggtgagt gagcgtatgg
    12181 ggacagaccg gcgagaggat caaggagcgg accgccggac tgataatggg accgccggca
    12241 gagagggaat aggcagtgga tccggtgggg gtgctgatga tgaggccatc accacgaagc
    12301 gtggtgacga attgtccttg aatcgcaata ttgagctcga tcatgcgtgc gagcgttccc
    12361 ttgctgatta cgacgtcatt cagcacgagc ccctgggcca ccgtttcacc atgccgatga
    12421 atgtgcgaac tgatcatcag gcgttcgtcg agcacatatt cattggcaaa cacccgctcg
    12481 agggaaggga acaagtgttc caacctgacc tcagtcagaa acccgagccc gcccatgttg
    12541 actcccagga tcggaatgcc gcgttcggcg gctaaccgcg ctgcattgag catggtgccg
    12601 tcgcccccca ggatcagcat cacgtcggcc ttttgcgcca actgagtttt ttgcacgcct
    12661 ccgcgctcgt tcaacaacgc agcggacgtg ctatccaaaa tgacatcaat ctttcgatct
    12721 cgcagccaat tgaccaccgc atgcacggtc gccttgatct ctgggaattt cggcttagtt
    12781 aatattccga tgcttttgct tttcatcgat ctgtagtacc gtaattcatc aaaattctgg
    12841 gaaaaggtgc gggattttat cgggagggtc cagtgaagtc aacgaacgaa cggtacgatc
    12901 tcagagggat cctgctcgat gtccgcgtgc atgccgtgca attctgtcgc aaccttgaca
    12961 gtgaaaaagc ttctagttag aattaggcag atatttcgaa gggtttgagc gactccccat
    13021 aggagccccc ccagaataag gaggaaggat gttcgaacga ttcacggaca agggtcggaa
    13081 gatcatcatc ctggcgcgtg aggaagccga gcggcaccaa aacgactacc tcggaaccga
    13141 gcacctggtc ttagccatcc ttcgcgaatc cgacggcatc gccctgatga tcctcaagaa
    13201 gatggggctt tccaccgaac agatccgcct ggaaatcgag cgcaatctcc ccgggggcgg
    13261 caccacgatg accttcggcg agattccctt cagcccgcga gtcaaaaagg tcatcgagta
    13321 cggcgtggag gaagcgcgcc tccttggcca caaccatatt ggcagcgagc atctcttgct
    13381 gggactgctg cgggaagaag agggtatcgg agggaaaatc cttcgcagcc tcggagccaa
    13441 cctgttgacc gctcggcaat tgacggtcac cttcctccgc aagtccgcac ccagagaacg
    13501 agaccgaaaa agcaacacgc cggcgctcga cgaattcggc cgcgacctca cgcaaatggc
    13561 gcaggaaggc caactggatc cggtgatcgg acgcgccgat gaaatcgaac gcgtgctcca
    13621 gatattgagt cgtcgcacga aaaataatcc agtgctgatc ggtgagtccg gcgtcggcaa
    13681 gacggcaatc gtcgagggac tggcccagcg catcgtccag tcggaagtgc ccgacaacct
    13741 tctgtcccgc cgcgtgatcg ccttggacct cggctccctc gtcgcgggca caaaataccg
    13801 tggccaattc gaggaacgtc tgaaagtcgt catgaaggaa atcgtccagg ccggcaacat
    13861 catcatcttc atcgatgaat tacacacgct ggtgggagcg ggtgcggcgg aaggttccat
    13921 cgatgcatcc aacatgctga agcctgcgtt gtctcgtggc gaaatccagt gcatcggagc
    13981 gaccacactc gatgaatacc gcaagcacat tgagaaggac ggcgcgctca agcggcgctt
    14041 ccagccgatt tatgtgcagc ccccgagcct tgatgagacc gttcgaatca tccagggtct
    14101 acgggaccgc tatgaagaac accatggtgt cgagattacg gaggatgcga tcgtcgaggc
    14161 ggtcaagctg tccgatcgct atatcaccga tcggttcctg ccggacaaag ctattgactt
    14221 gatcgacgag accggctctc gcgccaaact tcagacctat gcgctccctg gcgagctcaa
    14281 agcgatggaa caggaactaa aaaaagtctc ccgcgacaag gaacttgcga tttcgatgca
    14341 gaacttcgag gaagctgttc gtcatcggga agaggaagaa cggctacgca agctgctcga
    14401 tgaatccaag cgggaatgga agaaaaatca ggaaaagcac aaaccgacga tcggcaaaga
    14461 agacgtcgcg tacgtcgtat cgaaaatgac cggcatcccg ctcttcaagc tcgaggaaga
    14521 agaatccaac aagcttctcc gcatggagga gttcctccac aagcgcgtgg tgggacagaa
    14581 cgaggcgatt tcggctgtgt gccgagcgat ccgccggtct cgtgcgggct tgaaggaagc
    14641 gaagaagccc atcggttcat ttattttcct tgggccgaca ggcgtaggga agactgagct
    14701 ggcgagaacg ttggcggaat ttctgttcaa cacggaagat gcgctaatcc gaattgacat
    14761 gtccgaatat caggaaaagt ttaccagctc acggctgttc ggagcgcctc cgggctatgt
    14821 tggctacgaa gaaggcggcc agctcactga gcgcgtgagg cgtcggccct attcagtggt
    14881 gctgttcgac gagatcgaga aggcccatcc cgacgtcttt aatctcttgc ttcaggtcct
    14941 ggacgacggg gtcctgacgg atagcctggg ccgcaaggtg gatttcaaga atactgtcgt
    15001 catcatgacg tcgaatatcg gcaccaagat gatacagaaa ggggtctcgc tcggattcca
    15061 aagcacggat gccgagcagg atcggcacaa gaaggaagag gtactgggag aactccgtcg
    15121 gcaattcagc ccagagttcc tcaaccgcgt cgacgagatc gtggtcttcc accaactcga
    15181 caagacgcac ttgacgagca tcctcgacat cctgctgcgc gaactgaatc tgaggctggt
    15241 ggacaagggc gtcgaaatcg aagtcgacga cgaggtgaag gcctggctga tcaaggaagg
    15301 ctacgagcca ctctatggag cacggcccat gcgcagaacc atccagcgcg caatcggcga
    15361 tcctctctct gaggagatga tcaagggtcg attcaaggag agccgcaaga tccgcgtcgt
    15421 gctacgtgac ggcgcgccga cgttcatcga acaggaggcc atggcagagg tctaacgacc
    15481 gccgtgaccc tccatcacac ccatggcgaa gtcttgtgcg acccttgtcg gaatgacggc
    15541 aagggtcgca ttgtttttac ggcatgccgc gtctgtctcg aaacatgatc aaccgccaat
    15601 ccgcctctcc atggcaatca ggtccgatcc tgggtatcga aacctcttgt gacgaaactg
    15661 ccgcctcggt cctgtcctcc gatggatcgg tcctctccaa cgtcattgcg tctcaggatg
    15721 ccgtccatca acgtttcggt ggggtggtcc ccgaacttgc ctctcgtgcc cacgtcgaga
    15781 cgattgagtc cgtcgtaggt gcggcgatgg aagaagcgaa gctgtcatgg accgacctgg
    15841 cgggccttgc ggtcacccag ggacctggcc ttgctggtgc gttgatcgtt ggggtcaatt
    15901 acgccaagag cttggcgtac acactcgggc ttcccctcgt tggcgtcagc catttggaag
    15961 ggcatattgc ttccgcatgg ctggaggatc cggagtttcc gttgccctgt gtcgttctcg
    16021 tcgtgtcggg cggccatacg cacctctttc ttcaagatca acacggccgc tgtcgtctcc
    16081 tgggctgcac cagagacgat gccgcgggag aagcgtttga taagggggca cagatgctgg
    16141 gattgggatt tccaggcggc cctgcactcg atcggctcgc gcaaacaggc aacgcccgtg
    16201 cgttcgggtt tccgaagtcc cgcatcaaga aagacagcct ggaattcagt tttagcggat
    16261 tgaaaaccgc attgctgtac acgctccata gcatggagga acgtcaacaa gtcaaactga
    16321 aagccgattt ggccgcaggc tatcaagagg cgatcgtcac cgttctgatc gataaggcgt
    16381 tcatggctct caagcagacg ggcgttcgtg cgctggcagt ggtgggtgga gtgtcggcca
    16441 atagccgcct gagatgcctg cttcaagaac gagctctggc gacagacaca agaatcagcc
    16501 ttccgccttt acgcttctgt acggacaacg ccgccatggt ggcggcagca ggcagacaag
    16561 cccttatcgc cggtcgccgg atgccgttgg atgcagagcc atggtcgaca ttggaaccga
    16621 cgagcgagag tcctttcgct ataacccctg aggcctccca ttcctgagat ctacggtcag
    16681 accagcggat tgcgacccag tcaagtcacc gccatcgagc ggctctaccg gcgtcgcgtg
    16741 cctcacgaca aggtgctttc tcctgagctc ggcaggacca tgtgtcagat ctcacgcgag
    16801 attcgacgtc ctatcgcagt cctgctcagt agaaggggac aggttcaaga aatcatcgtc
    16861 ggaaccgatc tcacgctctc ggcgacgacc ctatcgaagt ttcgtgtcgg agcgcggtct
    16921 ctccgcgggc ttcgtgtcat ccggacacag ctccatgatc agccgttgag tcaggaatct
    16981 ttgaccgacc tcgcctatct gcgccttgat ttattcggag cgctatcagt gacgcctgaa
    17041 ggaaatcccg gcaacctgta tctggctcat ctcttgccgc caaacggcac gggtcaggtc
    17101 tgcaatatcc tcaagcacac gccgtttcat cacgccacca tggccttcga ccgctttatt
    17161 gaggatctcg aagcagacat gcaacgtgcc caagggactc acgcggtagg ccatgagagg
    17221 gaatcggcca tgctagtgag cgcctcatca cgaagccgag tcgaacaaga agatcgtctg
    17281 ctggaactcg cagagctggc ctcttctgca ggtgtcacgg tcgtcgaccg aatggcgcaa
    17341 cggacgcagg acggtcatca acgttacgtg ttggggagcg gaaaactgaa ggatgttctg
    17401 atccagacgc tccatagagg ggcggacatg gtcatcttcg atcaaaccct cacgcccgcg
    17461 caatcgagag cgatatccga aatgacggac attaaggtca tcgatcgaac ccagctcatc
    17521 ttggacattt tcgctcggcg agctcacaca cgtgagggaa aagtacaagt tgagctggcg
    17581 caactacgat atttgcttcc aagactttcc gggaaaggcc aggaactgtc ccggctgggc
    17641 ggtggaatag gctcacgcgg acccggcgaa acgaagctgg aaacggatcg gcggagaatc
    17701 agggaccgca tcacgcatct cgaacgcgaa ctcgatcagt tcggccgtca ccaggatcag
    17761 cggcgcgccc gtcgaacgag acacgggttg ccggtggtgt cgctggtagg atacacgaat
    17821 gccggaaaat cgaccctctt gaatgtcctg actcacagtc atgtctccac tgtggatcgg
    17881 gtatttgaga cacttgacac gacaagccgc cgacttcgat tcccacaaga tcgcgaggtc
    17941 atcattacgg acaccgtcgg attcattcgt gacttgcctg aagaactgct gggagccttt
    18001 cgcacgacgc tcgaggaatt gcgccaggcg gatctgcttg tccatgtcgt ggacgccggc
    18061 gcggctgacg tcgacatcca aatatccgcc gtggttaaaa ttcttcaaga actgaatctc
    18121 gacatgctgc ggaggacgct cgtcttcaat aaatgcgata gaattccgcc ggccgaggca
    18181 gaccggctgt gtcgtcgcta tcaggccatc ggcgtatccg cgctgcatcc cgacaccttg
    18241 cgcccgctcc tggaccatct cgaccgtgaa ctcgccgggg tggcacttcc tcaaccaacc
    18301 agccaggtcg accaagcagc tgcgcttgca tctcggttgt gacctcttca gaatctgcca
    18361 ccaggacatg cgacgtccgc caacatcgcc ggctgcgaac cggaagcgat tgcaacgcat
    18421 tgcgcaggcg cttgaggccg cgatgccgga ggctaagatc gaactctccc atcgatcccc
    18481 gtgggaactg ctggtggcca ccatcctatc ggcgcagtgc accgatcaac gggtcaacca
    18541 ggtgacaccg gcgttgttca cccgatacaa gacgccgtcc gaattagcca aggcgcgcct
    18601 accagatgtg gaagcgatca tccggtcaac tggatttttc aagagcaaag cgaagaacat
    18661 cgtggcctgc agccaagttc tcaccgagcg attcggccaa cgggtgccag aacgcatgga
    18721 tgatttggtc accctcccag gagtcggacg caagaccgct aacgtcctgt tgggccaagc
    18781 cttcggtcaa ccggcaatcg tcgtagatac acacgtgaag cgcgtggcca accggctcga
    18841 tgtgaccgga tcagaggatc cggtgcagat cgaacaggat cttcagcaac tgctgccgtc
    18901 atcgcaatgg acaagcgtgt ctcaacgtct gttgttgcat ggccgctaca tctgtctagc
    18961 ccggagaccc ttgtgcggtc gctgctcgat ttatgacgac tgctatgcca gggagaagct
    19021 gcctcgatga ttcagagcat gacgggattt gggcggaaac aagcgccgtg gcaggagggg
    19081 accattaccg tcgaggtgcg gtccgtgaac catcgatttc tcgagatgtc ctgcagattg
    19141 ccccggacct tggccatgct cgaggaaagt tttaagaaag ccattcagca gcgctgcacg
    19201 cgtgggcgtg tcgatttgac ggtgacgatt caagggacca aggggcgcag tggaaccgtt
    19261 gctcttgacc gtgcgcttgc aaaacagtac catcacgccc tccgtacgct aaaagcctct
    19321 ctcaaactga gcgggcctat tgatgtggcc ctgatcgcgg gactccgtga tgtcgtgtcc
    19381 gtctcagacc agcctcccga agagcctgaa ttggccagga tggttcaaca actcgccaat
    19441 caggcgttgg acgacctgca tcacatgcga aagcaggaag gccttgccct ggccaaggac
    19501 atgagaaaac gccttgagac gatgaaggat caccgatctg ccgtggcgag tcgctcgccc
    19561 atcgtcgtgc aagaaagctt cgaaaggatg aaagaacgca tcgccaagct gctcggaaca
    19621 gagccgcctg atcttccgcg attgcaccag gaattggccg tctatgccga ccgtggcgac
    19681 attacagaag aaatagtcag actggactcc catatgctac agtttgagca gcagcttaac
    19741 cgtgcggaat ccgtagggaa aacgctcgag ttcttgctcc aggaaatggg ccgcgaagtc
    19801 aatacgatcg gctcgaaagc aaatgatgcc gccattgcag ggcatgtgat tcatatgaaa
    19861 gccgaactgg aacgcatccg agaacaggtt caaaacgtcg aatgagcagg tccagcacca
    19921 cccctatgcc ttccggcccg gtccggtctt ccacggaacg gcggggaatt ctctttatta
    19981 tttccgcacc gtccggtacc ggcaagacca ccttatgcaa gcagctggtg gcgaacattc
    20041 ctggactctg gcactcggtg tcctacacga cgagaacgcc tcgtcccggc gaagaacacg
    20101 gccgcgagta ttattttacc gacgatacca ccttccaagc gatgatcgac cgacatgagt
    20161 ttgtcgagtg ggcgcgcgta tacggccacc tgtacgggac cccgagaagg atggtggctg
    20221 acaagatcga gcaaggtatc gacgtgttgt tggaaatcga cgtgcagggg gcgttgcaag
    20281 tgaagaagaa gttcgacgac gccgtctcga ttttcattct tccgccgtcg atgaccgtgc
    20341 tccgtcagcg cctactgaat cgcgcttccg attcctctga ggagattcag cggcgattgc
    20401 aaaaagtcaa agaggaagtc tggagctatc gggagtatgc ctacattgtt cgcaacgatg
    20461 atctcacgcg ctcgttacaa gatctcgaaa gtatttttct aggagaacgg ttgaaaacca
    20521 agcggctcaa tatggcctgg atcgaaaaca acttcattct ggacgacgag gccaaacccg
    20581 gagatcggaa agatcttcca taaccccaag gagtacagat ccatgatcga catgctcagc
    20641 ctgttacctc aatatacgcc ggaccaattt gactcccggc accgtctcgt cattgtcgct
    20701 gcgcagcgag cgaaacacat tgtgcaggga tcgcggccct ttgggatgtc gcgcttcacg
    20761 aaggagacaa ccattgccct ggacgaagtg ctgcgcgggg aagccaagta tctgatcggg
    20821 aaagaggcgc gggacgcgat gaaggaagcc aagcgtggca aagaaggcga gacggagcgc
    20881 atggccatga tgaccggaga agacgcgagg gaaatcaaga aagaactcag tgtctacgtg
    20941 gatgacagcc ccaagccggt ggcaggagaa ccagaagaat agcacatgga agaccatgtc
    21001 agaaaccctt cactcaccgg cgcacgcatt gtcctcggcg tcaccgggag tatcgccgcc
    21061 tataaggcgg tgacgctgct gcgtgcgctc gtgcgtgaag gggcggtgat cgacgtggcc
    21121 atgacccgct ctgccacgaa gttcgtgact ccgttgacct tcgaagttct gtctggacgg
    21181 ccggtcacgg cagacttgtt ccaggcgcac caggaaatga aacatctctc actgccggag
    21241 cacgccgacg tcattcttgt cgctcccgcg accgcaaatt tcctggcaaa ggcggctctt
    21301 ggactgggcg atgacctgct gtcaaccatg cttttgacgg ggcgatgtcc gctgatctgt
    21361 gctcccgcaa tggacggcgg tatgtggtcc caccccacgg tcgtcgccca tgtggaggcg
    21421 ttacgggctc gtggcgctct catcgttgat ccggaagaag gcccgcttgc ctccggtcga
    21481 atcggtcaag ggcgaatggc cggagaggac cgtattctgg acgccgtgcg tgcggtactg
    21541 actccgaaac gggactggca gggccaacgg atcttgttgt cggctggccc aacgcaagag
    21601 ccgatcgatc cggtgcgcta cctctcgaac cggtcgtcgg gcaagatggg ctatgccatc
    21661 gccgaagcag cacggaagcg gggcgctgag gtcattctcg tcagtggccc gacttcgctt
    21721 cccgcgccat caggagttga agtgatcccg gtagagacgg ctgaagagat gctgaagcag
    21781 ctcagcagcc gattatcttg gtctacggtg gtcgtcatgg ctgccgcggt ggccgacttt
    21841 cgtcccaggc aggtcgctcc tcaaaaattg aagaagcagg ggagacgatc cgctacgctc
    21901 gaactggagc agaccaccga cattctttcc acgatttcag gccagcgcac cactcaattg
    21961 gtgatcggct ttgcggcgga aactcatgat ctgctgcccc acgcccgagc gaaactcgcg
    22021 tcgaaaggcc tcgatctcat cgtcgcgaac gatgtcacca ggccaggagc tggatttggt
    22081 agcgacttca atgccgctac gctgatcgct cgagacggtc acacgacgga cattccgctg
    22141 aagtccaaac gtgaattggc ggacgacatt ctcgatgccg tacgccggtt actcgtggac
    22201 tctcctactc ggagacacgg cgatctccgc aaaaggcgct aacgatgcca cctcagaaaa
    22261 atatcgcgag cgccctggaa atcgatcgac tcgccacgca gctggcaaca aatccacgtt
    22321 cgaaggcttt tcttccgctt gcggaagaat attgcaaggc gggcatgtgg gaggaagccg
    22381 tcggcgtcct ggaagacggt ctgaagtatt accccgggtt catcacagcc atggtcgtgt
    22441 tgggacgcgc ctatgaccaa ctgaatcaac cgaccaaggc gagggcggtc ctcgagggcg
    22501 ccgtcaagct gagtccggaa aatctccgcg cgcatcggac tttgattaag atctatgcgg
    22561 cgcaaggact caccgaagag gctctcaaat cctgtcacgt gatcctcgca atgaacccac
    22621 gggacgagga ggtgctctcc cttcaagcct cattgggcgc tacagaatct gagaaaggcc
    22681 aaggggcccc atccccgaaa tcggtggagg tcacaggcca ggtgactgtc acccccggca
    22741 caaacaccgc gagcgggccc cctcccgccc tcgagtcggt cccgacgtcc attcctgaca
    22801 ttcccaccgg aaagctgcct agcccgctcg acgatcagaa gcctcctcag gatgtcgccc
    22861 cctcttctga ttccgacgtg gtgaccactg agtcacaaca gacgagtcat gctgataacg
    22921 tcgcacgcct cgagtcgtgg ttacgctcat tagagcggca gcgccgagat cgatcgacac
    22981 gagataccca ctccggctca taagttatta atatctttcg acagtttgac cgctctccag
    23041 tagcctgcta caatgcccct ctcctaacgc tgccggcacg aacaccacca ggcttttgaa
    23101 tacatcggct gatcatgctc cggatactcg tcttacatgg acccaacttg aatttgctcg
    23161 gtacccgcga acagtccatc tatggtaccc aatcgctcga gaatatcgac tcggccctca
    23221 cgacgctggc cgaacattta gggttgcaca tcgacattca ccaatcgaac agcgagggag
    23281 acttggtgaa ttggattcag gagggcggaa gcggctatca cggcatcatc atcaatcctg
    23341 ccgcctatac ccatacgagc gttgcgatcc gcgatgcgat ttccgctgtt ggcctgccca
    23401 ccgtcgaagt ccacttgtcg aatatccatc agcgcgaacc atttcgacat cattcccatg
    23461 tggcgggagt ggccttaggc cagatatccg gtctaggacc atcgggctat ctcctcgcat
    23521 tgcggggcct ccacgaccat ttagcaggaa ccaaaaagct caaaaattct ccgacggcgt
    23581 cccgccgaca gaaacgggcg tcacggtagg gaagcgtcaa cctcatagac ggtgaaggga
    23641 gtaacgagtg atttccacga ctgattttcg cggcggcgtc cgcctcttag ttgacggcga
    23701 gccgtattac atcgtcgaat ttcaacatgt gaagccaggc aagggcggcg cctttgtccg
    23761 aaccaagctg aaaagctatc tttccggcaa tgtgttggac cggacattcc gatcgggtga
    23821 gcgctttgaa gagccggacc tggaagaacg ggaaatgcag ttcttgtatg ccaccggcga
    23881 tgaatacacg ttcatggaca cggaaaccta tgagcagttg accttcgaaa aacagcaact
    23941 cggtgacaat gccgatctgc tcaaagagaa catgaacgtg aagattctta tctacgaaca
    24001 tcgtccgatc gacgtcgagc tgccgatttt catcgaactc aaggtcgtgg atgctgagcc
    24061 aggcgtgcgc ggcgatacgg cgtccggcgg cacaaaacct gcggtcgtcg agaccggggc
    24121 gaccatcaaa gtgccgctgt acttggaagt cggcaccgtc atcaaaatcg acacccgtac
    24181 gagggcctac gtggagcggg tccggtgagc cggaagaagc ctcgcagatc aaagcgccat
    24241 ggaaccccga tcgtgctgcc cgggtctttc gccggcgcga cgagcgccca agatgccgcc
    24301 gcgcagcaac ggcaaatcca ggacttgatc gacctcctca aacggaacca tttgacggaa
    24361 ctggaaattg agcgggcagg ggtacgcatc agagtccgcc atgaggccgc ggtgaaaacc
    24421 gtttccgcag ccgtcagtga agctgggcag ggcacgggcc aaaccgtctc accccacacc
    24481 agcccgccca atgaactcgc cgaagacacc gccggtcaca ttaccattac ctcgccgatt
    24541 gtcggcacct tctatcggtc gccttccccg gacgccgatc cttacgtgga agaaggcgat
    24601 ttcgtgaaaa gggggcaggt cctgtgtatc gtagaagcga tgaaactgat gaatgaaatt
    24661 gagtcggagg tggatgggcg catcacgaag atactcgtgg aaagcaccaa gcccgttgag
    24721 tacggccagg cgctcttcct catcgatccc aaagcgaccc cttgatccag gcgcgctcat
    24781 ctcacttcga ctcagtgaga acccttcccc tcgtgtcatg acggagatgt gaccgtgttc
    24841 aagaaagtgc tcgttgcaaa tcggggagaa atcgcactgc gcgtcatccg cgcgtgcaaa
    24901 gaactcggca tcaaaaccgt cgctatccac tcggaggccg atgcggccgg catgcacgtg
    24961 cgcgccgcag atgagcgcat ttgcgtgggc ccggccgagt ccgcgttgag ctatcgcaat
    25021 atcccgaacg tcctgagcgc cgcagaaatt accggggcgg atgccattca cccggggtac
    25081 ggcttccttt ctgagaacgc acacttcgcg gaagtctgtg agtccatcgg cgtgaaattc
    25141 atcggcccga cgtcagagaa catcgcactc atgggggaca aggccaaggc cagagagatc
    25201 gtggctcgtc gagggcttcc cgtcaccccc ggcagtccgg gcgagctgaa gagcgaacag
    25261 gaggcccgtg aggcggccgc caaaatcggc tatccggtta tcatcaaagc cgcggcgggc
    25321 ggtggcggtc gtgggatgcg cgtggtcaac agcaccgacg aagtggcccg ggcattccag
    25381 gctgctcagg ccgaagcgaa gtccacgttc ggcaacgaca gcgtgtacct tgagcggtac
    25441 tttctcgagc cccgccacat cgaagttcag atcgccgccg attcgcgagg ccacgtcgtc
    25501 tatctgggcg aacgcgattg ttcgattcag cgccgtcatc aaaaactcgt ggaagaaacc
    25561 ccttcgcctg ccgtggatga aaagctccga cgtgagatcg gacgagtggc ggttgaagcg
    25621 gtcaaggccg tccattatcg aaatctgggc accgtggaat tcctgctcga caaggaccac
    25681 aacttctttt tcatggagat gaatacccgc atccaggtag agcatcccat tacggaaatg
    25741 gtgacgggaa tcgacctcat caaggagcaa atcacgctcg ccaatggaca acccctctcg
    25801 ttcagacaac ccgatatccg actcacgggt cacagccttg agtgccggat caatgccgaa
    25861 gatccggaaa aattcacgcc gtcacccgga gtgattagca ggtactgtgc tccgggcggc
    25921 ttcggggttc gggtggattc ggcaatggag tccaacgccg tcgtcgtccc ccattatgat
    25981 tccatgatcg ccaaggtgat tacgcacggg cgcgaccggc tagaagccat ggccagaatg
    26041 cggcgggcac tcgacgagtt cgtcatcgag ggcatcaaga cgacgattcc gcttcacaaa
    26101 cggattctca atgatccgga cttccaaaaa ggccacgtct cgactaccta cctcgagcgc
    26161 ttcctcgcca gctagacgca agccattctc actcgaccat gatggtgccg ctcatgcccg
    26221 cgtggccgcg cctcgcgcaa taaaacagat acacgccggg aatggggcga acgagcgcct
    26281 cgactttctg attcggcggc acaggaaggc tgctggatgg ccccggtgac atcccgatct
    26341 gatgagccaa caatgccgtc ttgaattcat gccgctcgcg gccttcgttg acgacaagga
    26401 ggcgaatcgg atacgcggag gagacatgca cttcgctggg cgtgaatcgg aaatcctccg
    26461 cgacgatgcg gacggtctgt tccctcgatt cgcatccgga catggaagcg atgaggaaca
    26521 gaaccaccgc catcactgac ggcctcggcg actgctcggg caagggacag ctcatgtcaa
    26581 gcgggctgtt ccctggcgtc tgtttcatgt ctcacgctac aatgcaccta ccatgtcccg
    26641 gaaccaacgc acacgcacac tgtccggact atacgtcatt ctcgactcgt cgacgcggcc
    26701 caatcggccg ctcattgacg tgttgaacgg agccgccatc gccggtgtct cgatcttcca
    26761 gtatcgcaat aagacggcct cgatgaaaga cgcctttcgc gaggccctgc cgctccggaa
    26821 cagagcgaga gagctggagg tgctctttct cgtaaatgac cgttgtgacc tggcccttgc
    26881 tctggatgcg gacggcgtcc atgtcggtca ggacgattta cctgtggcac aggcgcgtag
    26941 gattcttgga cctgaggccg tgattggatt gtccacgcac agcgaccgac aggtggaagc
    27001 ggccgaccgt ctacaaccgg attacatcgg ctttggaccg atcttcgcgc ccggctcaaa
    27061 aaccgaccat gatcctattg tggggattga cgcactgaaa agaatccgcg tactgacccg
    27121 actgcccatt ttcgcgattg gcgggattcg cctcggtcag gttcgggcgg tcatcgaagc
    27181 gggagccgac ggcatcgccg tgatatctgc gctggccagc gcacctgaca ttgaagtcgc
    27241 cgtcagcgcc ctcctcgagc agatgtcctg accagcttgg ccagcatagt gatcgccgcc
    27301 atgtttgcga accgactcgc agactgccgg aatcgtcgca tgagacccgg cacatagggg
    27361 agtggaccga gcacaggcac gcccgcctgt ttccggaggg cgtccagagt cgtcttttcc
    27421 tgaagacgcg tgaccgacga tcggacagga tgcgtacgat tgaggatgag cgcgacaacc
    27481 ggaatccgcc gtcgatgaag cgcatcgatc gtcaacaagg catgattgat ccctcccagc
    27541 ccggcgcgcc ccaccacaat ggcgggcagc ttgagtccac gcatcacgtc catcagatcg
    27601 gcgtcggccg taatgggcac ccgaacaccg ccgatgccct ccaccacgac cgattgatag
    27661 cggttcgcga gcagacgata gacttgacga atcacgcgcg gatcgatggt ccggcgttcg
    27721 atatgggccg cacccagagg ggctaccggt tgttcaaact gatacggaca gatcgcgccc
    27781 agcgtttctt cgctgtcaat agcggcccga agccgtgccg catcggactg gatgagccga
    27841 gaagaagtga cgccggtttc aatcggcttc atgatgccca cggtcctccc cagacgccgc
    27901 aaccggatag ccagtgccgc tgtcaccagg gtcttgccca cgccggtatc ggttcccgtc
    27961 acaaacacgc cggccccgcc tgtgactcgt cgcggtatca tgcaaatagg ctccggctgt
    28021 cacagttcca gacctgaccg gagacgtcct tcaactccac gagatgcatg accgtacgca
    28081 cgacttcctc aagtgcaggg ggccgcccga gagcatggtc tctccactgc tgccccctgg
    28141 acattgcttc cgtcgtcaaa cgtgtgtgct gccaccctgg caggacgaga ttgatacaaa
    28201 tgttgtccgg tccccattcg accgccgccg tcttgaccaa cccaaccagg ccggctttgg
    28261 aggcggcata ggcggcttga ccggtcgatc cctgagatcc ggcgtgcgag ccaagaacca
    28321 cgatggatcc gcctccttgg gcgaggaggg caggggccat tgcccggaga gaatggaatg
    28381 tgcccgtcag gtttgtcgcc atgacgtccg cccactcctc cgtggagagt ttcaacaata
    28441 agcttccgcg tccgacgccc gcgcagcaca caaaacacac aggccgcggg ttggctcgaa
    28501 tgaacgccgc gatcaagtgg tgcaccgctt gagcatcccg cacgtcggct tcatagagct
    28561 ggccgctgcc cccggccttc tgcacgtcgt gcagcgtcga ctcggctgca agtttttccc
    28621 gatgatagtg gatgcccacg caccagccgg ccttcccgaa cccgcggctg atggcctggc
    28681 ctatcccgcc ggaagcgcca gtcacaagga cggcccgcgg ccctgctctc gcctgacaac
    28741 tcgctgttgc gctgggacga gtctccatgg gcaagggatt atgggtgtgc gcaaggcacg
    28801 aaggcaagcc tgcccgagcc cgatgaagtt tctccgccct tgctgccccc acgattcatg
    28861 tgataccgtt tcctaaacgc tgagcttctt gcccttatgc ccacaggagt tggccacaac
    28921 gtgcgattct gcagcctgac cgtcttgtgc ctggcgctct cttggtcggt ttcacttgcc
    28981 gccgaagagc tcaccggcat cgtgcaatca tcggattatt ttcctgacac catcggaagc
    29041 cattgggaat atcacggtca aatcactgaa gggccgcttc aaacgatcga acacaaattt
    29101 tttaccaatg tgtccacggt agtcggaacc aaaccccttc gagggatgac cgtcacggtg
    29161 tttcatgata ccaaccccgg caatcatggg ccctccgaca gcttctatcg gcgcgatgtg
    29221 gtcggcatcg tgtaccatgg gtccgatccc ggcactcccc tggagaaaca gctgattccc
    29281 tatcagatcg tccgctttcc gatacaaatt gcctcgacca tccagcaatt tgataagaaa
    29341 ggcctcgatt tcggaacgga catggatcgg gacggcgtga atgagaaagt ggacgcgcat
    29401 ggcacctcga ccgtcgctgg gttggaatcg attaccgtcc cggccgggac cttcaaggat
    29461 gccgtcaagg ttgaagccta tatgcgaatg aaaattcatc tctccggagc ctcacgaacc
    29521 gtcaccggca cggatgtgat gaccgcctgg tttgccaaag gggtcggact gatcaaatat
    29581 gtggagcggc aggaactgtc tccgcttgaa gaccgaggcg tgattacgga aatcatggag
    29641 gagttggatt catacgaagt aaaatccccg aatgcatcac tcggccggag cgaaacccct
    29701 gcggagcgtt tgttcgctca cgattctcgt caccacgaat tgcaacaagt aatcttcccc
    29761 tccagctttc gcccccaccc ctgacagccg gtgcccgcca aaaggttggc ggttgacgag
    29821 tgcaccggtg atgggtcggt tcaagtagag atttccgaca tcgaatcgca gccgcgccaa
    29881 gtcgagattg gccgggctgc gcgagtagac tcccccggtg agtgcatagt ccgtcccatt
    29941 cgcgactgcg agcgcttcgt cgaacgattc cgctctcatc accgccagca ccggcccgaa
    30001 gatttcctct tgtgcgatcc gagcggtggg tttgacgtcg gcgaaaacgg tcggcgccac
    30061 gtaccaacca ggctgcggga cccggccttc aaccaccaca cgcgcttcct gactgccgat
    30121 tttgatgtac tccaggatgc tggcctgcgc ccgctcgtcg atgaccggtc ccatctgagt
    30181 tccgggttct tcaggattgc ccacccgcag gctcgcgatt gcatctcgaa gccggctcag
    30241 aaagaggtca tagaccgcgg cgtggacgat gacgcgtgaa caggcggagc acttctgccc
    30301 gctataggag gtgctggatg ccaccacacc ggcaacagca tcgtccaagt cggccgtttc
    30361 gtcgacaatg atc
//
LOCUS       DASSCQ010000105        21617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_561101, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000105 DASSCQ010000000
VERSION     DASSCQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 21617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21617
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561101"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFH05_07625"
     CDS             complement(<1..349)
                     /locus_tag="VFH05_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678120.1"
                     /translation="MKSALSILFGVAAVAFVAAPVTSFAGGTIAGKVTYAGKPEKKEF
                     SFGKFPNPKFCPKNPNKALIDGDKRLMPTIEVGKDGGLKNAVVAVIDVEDKAFVDGYK
                     GTEVIAEFCEFLPY"
     gene            complement(572..1909)
                     /locus_tag="VFH05_07630"
     CDS             complement(572..1909)
                     /locus_tag="VFH05_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6678121.1"
                     /translation="MTRTAMQWIVLGLLVSVLMGAGLSSVPPDIKAWYDQKEYQKVLD
                     GLSALSQDAQALPDVRRLKARTLVKLGKPQEALAEYDKLETGLGRDDVPLLRQVALGF
                     ILVLLKDMREQMRGAAYTALKEIDSDEAVPYFEDGLSDGSGPVRVLAVEGLARSDAGR
                     KSKKLRLALEDQAGVVKARAVKALGKSGDRSVLPLIEHATKDELATVRIAAWGALIRL
                     GRTEAWEDLRKAGEAVNPEERAEALRTMADLNDQRAVPLMHSLLNYNQPSVRAAAARG
                     LGHLHKHDAREDIEKLLEDPVGPVRESAAAALADLGATESVVALTRKLSDGAFTVRAA
                     VGAALLQLSQPFDSVGPTMLALAQQNDPAARSSAALALGKATKTNREGAIAVLTGLAT
                     DPLPGPRIVALRSLGRIGDQSVVPDLKAGLHDQNEAAKATAAGALLHILPPLK"
     gene            complement(1913..2548)
                     /locus_tag="VFH05_07635"
     CDS             complement(1913..2548)
                     /locus_tag="VFH05_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678122.1"
                     /translation="MWTSRTWKFQALWLACIVNALTDTPIVPASAGTKAFPPHEFSGT
                     ITKQVNGKKYQAQVFAKADRLRLEYKYALRTDYGYAGIEIVRLDLAEVWYLLVQQKQL
                     LVTPLDPDDVLPMQPALPGEHSRTLIGDATAVERAAQLYEVQTDRRGRTERWYEWVDV
                     ETGVVLKLVSRDRDWSFAYERIRWSPQPSQYFNEPPGYQKRQVPAAHGQRG"
     gene            complement(2611..3549)
                     /locus_tag="VFH05_07640"
     CDS             complement(2611..3549)
                     /locus_tag="VFH05_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET6678123.1"
                     /translation="MNLRKHILRMSLVTAMSLVTAMSVSTGAALGYDVIDVEHGGTIE
                     GTVSLEGAVPEPKGFNLITFPDPQYCGRISNGRGWRLLHDFVVGQNGGLKDAIVLLEG
                     VEAGKPFEVSVPLIEARDCMFQPFMTIVRNGHAVEVINMDPVMHDIQAYETSIEAGAR
                     VLFNTPLVMNHQHNRGDLHAIHNHAPGKSLVGPIYLNKGRRTFYMQCGFHAYMESWAM
                     AVNNPYYALTDENGKFSIEQVPPGTYQLVVWHPQTGPGLIKTVTVKADGKLDEQLSLP
                     APKGNRSAYKVLDNPRFGPESLGRSIEIDPFVEHQH"
     gene            complement(3613..4560)
                     /locus_tag="VFH05_07645"
     CDS             complement(3613..4560)
                     /locus_tag="VFH05_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6678124.1"
                     /translation="MNKLHRSHGLLEALMKGLLFVFGWLLLGSATALAYEEIQVADGG
                     TVTGKVTITPGKPIPKGFNLVTFPDPVYCGRISTGTGWRILKEFEVAADGALKDTVVW
                     LADATKGKPFKFETPTIEARDCRFLPFVTIVRDQADVVIMNMDPVMHDIQAYETSQLG
                     PRVLFNTPLPMNPHHKRNVGADTHEHLAGQPITEQIRMTKGRKFFVMQCGFHAYMESW
                     GLAVDHPYYQLTGHDGRFSLTDVPPGDYTLMAWHPGVGTVLEKKVTVPAKGTAQVDYV
                     FDSPRGRRSAHEIEENPHFGPDSLGKALDIRPTLELQKP"
     gene            complement(4653..9494)
                     /locus_tag="VFH05_07650"
     CDS             complement(4653..9494)
                     /locus_tag="VFH05_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET6678125.1"
                     /translation="MFLHFTRVYRPTCTLGVLAAAALLWAPFASAEDPSSHAAMNHQL
                     PAWAEQLKGQTIIEDTMSGKGERAAMVEQQHQRIMEHMAQDPQMQGVNTGMYNTSAMM
                     HQYGAGGQDMLLVSDARVEPVAMTGGGKCPATAPVKQYNVSAVNVEITLNQWLDFYPG
                     YMYVLDEDIDKVRAEENTNKEARDKEGHDPGAVIPGVQAQWIQPLVIRGNQGDCLKIK
                     LSNKLDGGEEVSLHIHGSAMVVSATGAAATTTNPDTIVAKDKTGEYEWYIHPSTQEGV
                     RQFHTYSNDRELTVMGLFGAFVVEPRGSSYLEPLGTGDPTPATSGWQTIIKNGTGPDF
                     REFVLIYHEVGDEAFRPLNKKGDFLPQRDPLTDVYRPGGRSINYRSEPFGINNMHVQH
                     EYFGFEDESMGYSSYTFGDPSPTIPRSYMGDPAKFRLVHGGSEVFHSHHPHGGTIRWP
                     RSPRAIDDMNLWATSTNGPVKYPVIRAKTDRVDVEVIGPSEALDLETECGSGLCQQLA
                     GDFLFHCHVAHHYVAGMWGYWRVYNTIQQGDMRNDVMPDLRELPDRKGRIKAPISSDK
                     LIGQTVDWFGTQFKIIEKGKSNWKGNPATITIKDWVEMQMPTAGKPGHKDDEKGQIVS
                     YDATVLDWTWNGNVAMSEKESTIDNPKYKSTHPGKRHPIMFEPLTGKVAWPHLTPHFG
                     KRVMFSPNHVGAPWLEMIRREANGEESVEQAAPGEQGNWSLCPVNAGRKSYNVHFIKL
                     PIKIAKAQGKEPPVVDPNGLIYVLHEEEAAIRANDDLKYPLVVRGNIYDCVDWTLTSE
                     WDDDDYTNFQSSKINTHWHFLQFDNQASDGVITGFSYEQSVRPFTMLEKKNKKGLPAP
                     MNTVLTAAAKKGSNSLSVKNAKQYHVGTLILVGADNVKGNEISRIKAINGNTITLAKG
                     LKNDHPANDIVTVEFVRQRFWVDADVGTVFWHDHAFGATTWPHGGFGTFIAEPVGSTY
                     HDPKTGKPVRSGPIADIHTNEPVGHGVNNSFRELMVQVHDTVPHTVNIVTAGNPPGQP
                     VEVALEAGKTVSFMMPEKIYMTPMPFLNGGTHTTGSGLNFRAGPIAQRLATNPDVSQA
                     FNSQIHGDPYTPLLRAYTGDTMVFRLLHTLMNETMTWTLAGHTFWTERYASDANRKNS
                     IHIGIAERYDLVVPEAGGPRHLAGDYIHFNGRSSKFSEGAWGLLRVYDKEQPDLKKLP
                     AGFSAKGEIPKALPVCPSDAPVKNFNVVAMDYPAMTFNPKAPEAIEVDFERKIQISNP
                     DAKIYALEEDAAKVSGGSHPMPLTLRVNVGDCVKVKLKNKMKEGKASFSAIGLVFDPK
                     ESMGANVGNNPGDQTIAPGGERTYTYYADPFNGETTSLVWDWGNVMTNPRNGLFGAVV
                     VGPKGSKYRDPKTGADLSNKNAWAADVIIDRSIPGNESKANYRDVALFFQDEDNIIGT
                     SFMPYVQNVAGLTGVNYRSEPYKYREEQGCSLGKVFQPCKADKPEDPATPIIESHAGD
                     PVRIHVIGANNEQNGMFSVEKHEWPIEPYMRGADQISVVEYSGSEVLDAFVPSAGGPY
                     RLPGDYVYSNQRLPYSQSGQWGYLRVLPAGDQRLLPLAGAGAGMKSASLEEAAQVIPV
                     NAK"
     gene            9832..11649
                     /locus_tag="VFH05_07655"
     CDS             9832..11649
                     /locus_tag="VFH05_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6678126.1"
                     /translation="MLLKRWLVGNPIKTAHAAHERLSKSLALAIFSSNSISSVAYATE
                     EILLVLILAGTAALAWSIPVSLAILFLVIVLTISYRQIIYEYPEGGGAYIVARSNLGE
                     MPALIAAAALMIDYVLTVAVSVAAGVAAITSAIPELFAHREALGLISIVFIVVVNLRG
                     TRESGKFFAIPTYFAIGALGLMVLMGTVRSFTDGVAMLPLPSPAGGDLENLSLFLILR
                     SFAAGCAAVTGMEVISNGVKAFRSPESENAARTMVWMSVILGSLFMGISWMAYHYGIM
                     AKEDETVVSQLARLTFGTGVTYYTIQVGTMMLLVLAANSAFAGFPHLASILARDGFMP
                     HQMATFGDRLVFSNGIIILGFFACLLLVVFQGDTHALIPLYAIGVFVSFTLSQAGMVR
                     RWLVKKGLHWRKKLIVNGVGAVTTGIATVIIAATKFMQGAWIVFLLVAALIMIFRGIR
                     RHYKAVAEQVMLTRDARPPRPRRNLIIIPIGAVNKAVVRAVDYARSRGGEVRAVLIDV
                     DKEETAVVEIKWAQWGCGVPLIVLPSPYRSILGALLDYVEENLDKDPDCWITVVLPEI
                     LPARWWQNILHNQRALLLKGALLFKDRVVLTDVPFHLTR"
     gene            11652..12728
                     /locus_tag="VFH05_07660"
     CDS             11652..12728
                     /locus_tag="VFH05_07660"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET6678127.1"
                     /translation="MKPQYRLQRTITARMRRLIILLQGFVFVLVTGMLADHAQAFKIT
                     EPAAGTKLGAGQTVTAHVDLGNDTGIIKVRYYWYGEQDDTLVEQDDATAMGSIIAPVA
                     LVGLSGHDPAFGGKLVVPKEGIGPMRLLAVAEISRGRLGSRSVFDEILVHVEPKAALT
                     TIDFETDKPLQLGRSGQSSAFGHVDSMGKIFELPVVGDFADGVTRRISAPSTGTSYDS
                     SNPQVIKVLPSGLLQIVGNGKTVLTVKNRDKQAQLDVDVNVNEEPNEPPVADAGDPKT
                     VKAGTKVKLSGLRSRDAEGEALYYAWSQVRGSKVALLDVNGPEATFQAPQVSEARTYR
                     FKLRVTDRKGADSVPAFVDVTVEP"
     gene            complement(12804..14369)
                     /locus_tag="VFH05_07665"
     CDS             complement(12804..14369)
                     /locus_tag="VFH05_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET6678128.1"
                     /translation="MSKRWVQLALGCGLVVVVVVVASAFVDEPLRAYVEQRMNRSLKG
                     YTVRIGALDFHPIGFSVDLEDVELVRTDEPDPPMATIARWTARVHWKALLSGRVVNDQ
                     YMERPRFHITRRLARKEVGDERPVRDRGWQDAVESIYPFKINQVRMANAELTYIDEAK
                     PERPLHFDHLEVYATNIRNVQSGDQTYPSELQLEGQVFDEGKLRVDGHADFLAEPYVA
                     VKADILLENVPLTALLPVSARYNLQLSGGSMSAEGSVEYAPAKKLVSLKRLRIDGLHA
                     DYVHARQTQGAEVARARATVGTAQQVHASEDTLVRVEEITIVNSEFGFVNQAAKPEYR
                     VFLADAEAALQHYSNRLQEGAASVQLRGKFMGNGETSVTGILRPEHESPDFELRVKIA
                     GTPIRAMNNLLRAHGKFDAVGGTFSCYSEVMLEDGRIRGYVKPLVRKLNVYDEAQDRH
                     KNPLEKVREGAIEDLSTLLENVPRDEVATKVDMSGSAQRPRTEIVQIVIGLIQNAFFK
                     AILPGFEQEFRSK"
     gene            complement(14486..16039)
                     /locus_tag="VFH05_07670"
     CDS             complement(14486..16039)
                     /locus_tag="VFH05_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HET6678129.1"
                     /translation="MSLLDTAQLRRVQHEFRSSMRRLLRDLCHDLERHYIGLASGLGL
                     PIEYFLFLTQAFEGSSYSNWKVVGWIEALNDLVYFLDVLAQIRKERNPQLFAQQLFDE
                     CEQKFFENSYTDDLFPQRQARAFGLEGRLHRLCVRQAREVTEESLFFDPQWPMAWLAR
                     EGRQTWEVPAGLDHCFERGERAGTVPIGTQGIALLVPRVVARDLRGRRRRPTWIVTPN
                     DITLRLGRSRPLCRRRGNRIEYHCSLTELLVAANTPYGPLTVGPTLHYGKTQEPTALS
                     ATAPRQVARIARAWKAIRLAWPEAHRVLELLTSRIVPLKARGVVSFSYRHRPGLSFVN
                     CFHRDNLDLIDDLIHENSHHHLNLLLRKHVLYRDDHNQQIFYSPWRRSLRPVRGILHA
                     TFTFTMGAQLFERLSSWAETRSGLAQWRETGLSSGDLLRARFRCLEEIESVRYSLRDL
                     QYARDRLKWLTGSGARLVRHLEQAIAKAERNISKHRHTVLDSSYGRLLEAHQTELRQA
                     RHTYGPRHG"
     gene            complement(16145..18871)
                     /locus_tag="VFH05_07675"
     CDS             complement(16145..18871)
                     /locus_tag="VFH05_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6678130.1"
                     /translation="MPGSTHSAGEAAAYLAAVVDSSHDAIITKNLSGIIQTWNKAAEH
                     IFGYTEQEAIGQSILLIIPPEFHPEEASILERLRKGDRIENFETVRRRKDGRLIHIDL
                     TVSPVRDGNGTIIGASKIARDITERKQAKQVIAEAQVRLATTLESIGDAVLATDTESR
                     ISYLNPVAERLMGCSIEEIIGQPLDKFFTIVNAETRQAVPSPVERVLREGTVAGLANH
                     TVLLRRDGTEVPIDDIAAPIKDADGRLLGVVLVFRDVSDQYKAARQAVLLSSIVASSD
                     DAIVSKDLTGTITSWNRGAERMFGYTAEEMIGSSILRIIPTDRHSEEAAIVSKLRRGE
                     RVDHYETIRCKKDGRLFDVSITVSPLTDDDGNVIGASKIARDISEQKRTQRALHESEE
                     RWRVTLESIGDAVVATDASAVVTFINPIAADLLGMPAAEIMGRRLAEVFTIVNEDTRQ
                     AVDNPVERVIRDGLVVGLANHTVLVRPDGTEVPIDDSAAPIRDHDGRLIGAVLVFRDI
                     SGRKSSELQVQRWNTELEARVRERTGELVKSQERLRALASQLNLTEQRERRRLAGDLH
                     DYLAQLLALIRIKLGQALQRLNDRAPEVRSALTEMDTLLQQSLQYARTLMAQLSPSVL
                     QDLGLIPAIHWLAEQMAQQGLTVDVRVLTDEQPGFSEHQSDLLFQALRELLTNVVKHA
                     GVSSAVVTVNKQDRDTWLITVQDSGAGFDLNAVQYLPSGEHFGLFSIQERMEAMSGWC
                     RIHSLPGEGTTVELGLASMPADASRKLHVHRTAVGVADPSRAAVPSRWRVLLVDDHAM
                     VRQGLRSILETYSDLEIIGEAADGLEAVESAKSTQPDVVVMDINLPKLNGIDATKRIK
                     NYAPNAVVIGLSVQHSAQTLNAVLDAGASALLSKEQATVDLYRTITHFLKVR"
     gene            19250..20203
                     /locus_tag="VFH05_07680"
     CDS             19250..20203
                     /locus_tag="VFH05_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6678131.1"
                     /translation="MATRWYDAFKARSLWHLLKEAAFDWSHDRAPRLGAALAYYTVFS
                     IVPLLIVIIALIGLFFGEDAAQSAIMDQISNLVGEQSAAAIKDMIQRAEQPSTGVVAT
                     VVAVVTLLFGASGVFGQLQDALNTVWGVEPKEGRGVWGFIKDRFLSFVAVVGTGFLLL
                     VSLVLSSALAALGKWFSGVLPLPEVVLQALNFMVSFVVITGLFALIFKVLPDARVAWR
                     DVWVGAALTAALFTIGKFAIGLYLGKSNVASAYGAAGSLVIVLVWVYYSAQILLYGAE
                     FTQVYANRLGERIVPAPDAQVANPQKASNLEGNKREAGRAA"
     gene            20319..20891
                     /locus_tag="VFH05_07685"
     CDS             20319..20891
                     /locus_tag="VFH05_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6678132.1"
                     /translation="MWYRRELRVYCYPLEGSPPLPRPAIFERNRFDDLKYYERTDKYQ
                     PSPEVYRKMALERKTQGFHLYTFVQERRLLHYGWLIERQTRGEDDWVQQAYFPPPDTS
                     VLFDHFTHPSARGKGLYFQALCRLLHDARDLSGARQAYVTVFGSNAPSRHVIEKVGFH
                     HEGSLYKERKLFWSTRYSVGTHDDFRTALL"
     gene            complement(20901..21179)
                     /locus_tag="VFH05_07690"
     CDS             complement(20901..21179)
                     /locus_tag="VFH05_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678133.1"
                     /translation="MKRRPSQDVWEAFHIVTDAAENFVNRSPRSKRLETERQALLDAI
                     RRAQLVLSVNRLAPPSSASDPTVPSLANFRARREDVKESSRTRRQRRE"
BASE COUNT         4344 a         6398 c         6012 g         4863 t
ORIGIN      
        1 agtacggcaa gaactcgcag aattcagcga tgacttccgt gcccttatat ccatccacaa
       61 aggccttgtc ttcgacatcg atgaccgcga caacagcgtt cttcaagccg ccatctttgc
      121 ccacttcaat ggtcggcatc aaccgcttgt ctccgtcgat caacgccttg ttaggattct
      181 tcggacagaa tttcgggttc gggaacttgc cgaaggaaaa ctccttcttc tcaggcttcc
      241 cggcataggt gactttgccg gcaatggtac cgcccgcaaa ggaggtgaca ggtgccgcga
      301 caaaggcgac ggctgccacg ccaaatagga tggataacgc actcttcatg ttactgcctc
      361 cttgtgatta acgaaccccc gacaattaat gattcctagc tcgctgtccc ccgtcttcat
      421 tccggttacg agaggatcgt cagaacgctt ccctccctgg gtactcgatt gacgactggc
      481 tggcgaagct ggtcgtagac cgagtacata tactgaatgt tactgcgtga tgtcaaccag
      541 ttgcaagctc gctatgggca cctgttggca cctatttgag cggcggtaat atatgcaata
      601 gagccccggc ggcggtggcc ttagccgctt cgttttgatc atgaaggccg gccttcaggt
      661 ccggcacgac ggactggtct ccgatgcgcc ccaacgagcg aagcgccacg attctcggac
      721 ctgggagcgg atccgtcgca agacccgtta acacggcaat tgccccctcg cgattggtct
      781 tcgttgcttt gcctagagca agagccgctg aggatcgtgc cgccgggtcg ttctgttggg
      841 caagcgccag catggtgggt ccgaccgaat caaacggctg gctgagctgc agcaacgcgg
      901 cgcctactgc agctcgcaca gtgaaggcgc cgtcagagag tttgcgcgtc agcgccacga
      961 ctgattctgt tgctccgaga tcggccagcg cggcagcggc ggattcccga accggtccta
     1021 ctgggtcctc aagcagcttt tcaatgtctt cccgcgcgtc gtgtttgtga agatgcccaa
     1081 gtcctctggc ggcagccgcc cgaacggatg gctgattgta attcaagaga ctgtgcatca
     1141 acgggactgc ccgctgatcg ttcaaatctg ccatcgtgcg aagggcctcc gctcgctcct
     1201 ctggattgac cgcctcgccc gccttgcgta agtcttccca ggcctcagtg cgcccgagtc
     1261 tgatcaaagc gccccaggcg gcaatgcgga cggtcgccaa ttcatccttc gtggcatgct
     1321 caatgagcgg gagcaccgac cggtcgccgc tcttaccgag cgctttcacg gcccgtgctt
     1381 tgaccactcc cgcctggtcc tccaacgcaa gtcgcagctt cttggatttt cggcctgcgt
     1441 ccgagcgtgc caatccttcc acagccaata cgcggaccgg acctgagcca tcactcagtc
     1501 catcctcgaa atacggcaca gcctcgtctg agtcgatttc cttcagtgcc gtataagcgg
     1561 ctccgcgcat ttgctcccgc atatccttca gcagcaccag aataaagcca agcgccacct
     1621 ggcgcagcag cggaacgtcg tcccgtccta gtccggtctc tagcttgtcg tactcggcca
     1681 gcgcctcttg aggctttcca agtttgacca gcgtccgggc tttcaatcgg cgaacgtccg
     1741 gcaaggcctg tgcgtcctgg ctcaacgccg agaggccatc gagaaccttc tggtattcct
     1801 tctggtcgta ccacgctttg atgtcaggcg ggacggatga caatcctgcg cccatgagaa
     1861 cggagacgag gagcccgagg acaatccatt gcatggcggt ccgagtcatc gcctatcccc
     1921 gttgcccgtg agccgccggg acttgcctct tctgatatcc cggcggttcg ttaaaatact
     1981 gactgggttg cggagaccat ctgatccgtt cataggcgaa cgaccagtcg cgatcgcgac
     2041 tgacgagctt gagcaccacg ccggtttcca catcgaccca ttcgtaccac cgttccgttc
     2101 gaccgcgccg atcggtttgg acttcgtaca attgtgccgc acgctcgacg gcagtggcgt
     2161 caccaatcag tgtgcgactg tgttctcccg ggagggcggg ctgcatcggc aacacatcgt
     2221 ccggatcgag cggtgtgacc agtagttgtt tctgttggac cagcagatac cagacttctg
     2281 cgagatcgag ccggacaatt tcgatgccgg cgtaaccata gtccgtacgc agcgcatatt
     2341 tatattccag cctgagtcgg tcggccttgg caaacacctg cgcctgatat tttttcccat
     2401 tgacctgttt ggtgatggtt cctgaaaact cgtgaggagg gaaggccttg gttccggccg
     2461 atgccggaac aatgggtgtg tcagtgagcg cattgacgat acaggccagc cagagcgctt
     2521 gaaacttcca cgtacggctt gtccacatag gacggtgact gttcggcaga accgccgccg
     2581 ggaagccggc tgccgcacca tgcacatccg tcaatgctga tgttcgacaa acgggtcgat
     2641 ctcgatcgaa cgcccgagcg actctgggcc gaagcgcgga ttgtccaaga ccttatacgc
     2701 agaacggtta cccttcgggg cgggcaaaga caactgctcg tccaattttc cgtcggcctt
     2761 cacggtgact gtcttgatca ggccagggcc ggtctgcgga tgccagacga ccagttgata
     2821 agttccaggg gggacttgct cgatggagaa ttttccgttc tcgtccgtca gcgcataata
     2881 cggattgttt acagccatcg cccagctttc catgtatgca tgaaatccgc actgcatata
     2941 aaacgttcgt ctccctttat tcagatagat tgggccgacg agtgatttgc ccggtgcatg
     3001 attgtgaatc gcatggagat ccccccgatt atgctgatgg ttcataacca ggggcgtatt
     3061 gaacagcacc ctcgcccccg cttcgatgga ggtttcgtat gcctggatat catgcatcac
     3121 cgggtccatg ttgatgactt cgacggcatg cccgttgcgc acgatcgtca tgaagggctg
     3181 aaacatacag tctcgcgctt caatcaaggg aaccgacact tcaaagggct tccctgcttc
     3241 caccccttcg agcagaacaa tcgcgtcttt caacccgccg ttctgtccca cgacgaaatc
     3301 gtgcaacagc cgccagcccc gaccgttcga aatccgtccg caatattggg gatccggaaa
     3361 ggtgatgagg ttgaatccct ttggctcagg gacggcgccc tcgagcgaga ccgttccttc
     3421 gatggtgcct ccatgttcca catcaatgac gtcgtacccg agcgccgcac ccgtgctgac
     3481 gctcattgct gtgacaagac tcatcgctgt gacaagactc attcgcagga tgtgtttgcg
     3541 taagttcacg atgtgctcct tatgaaaatg gatgaatgag cagtatcgga agaaaataaa
     3601 tcggcacctg ccttacggct tctgcagttc cagcgttgga cgaatatcca aggccttgcc
     3661 gagtgaatcc ggtccaaaat gagggttctc ttcgatttca tgcgcgctgc ggcgtcccct
     3721 aggagaatca aacacgtaat cgacttgagc cgttcccttg gctggcacgg tgaccttctt
     3781 ctcaagaacg gtaccaaccc ccggatgcca ggccatgagc gtatagtcac cgggcggcac
     3841 gtccgtcagc gaaaagcggc cgtcatgacc cgtcaactga taatagggat gatccacggc
     3901 aaggccccaa ctttccatat aggcgtggaa accgcattgc atgacaaaaa atttgcgacc
     3961 ctttgtcatg cggatctgtt ctgtgatcgg ttgacccgcc aaatgctcat gagtgtccgc
     4021 tccgacgttt cgtttgtggt gggggttcat cggcaacgga gtattaaaca gaacccgtgg
     4081 accaagctgg gacgtttcat aggcttgaat gtcgtgcatg accggatcca tgttcatgat
     4141 gaccacgtca gcctggtctc gcacaatcgt gacaaaggga aggaatcggc aatcccgtgc
     4201 ttcgatagtc ggcgtttcga atttgaatgg cttgcctttc gtcgcatccg ccagccagac
     4261 gaccgtatcc ttgagtgcgc catccgccgc gacctcgaat tccttcaaga tgcgccaccc
     4321 ggtccccgtg gaaattcttc cgcaatagac tggatctgga aacgtcacca gattgaagcc
     4381 tttggggatc ggcttgccgg gcgtgatcgt gacctttccg gtaacggtac ccccatcggc
     4441 cacctgaatt tcttcgtaag ctaaagcggt tgcactacca agcagcagcc aaccgaatac
     4501 gaacaacagc cccttcatta atgcctccaa caatccgtgc gaacggtgca atttgttcat
     4561 cgtaccaaat gatcacggca aagaaaaagg gggaaacctt tcggcttccc cctttccctt
     4621 cttcgccttc ccttactggg aacggtacga cactactttg cgttgaccgg aatcacctgt
     4681 gccgcctctt ccagtgaggc gctcttcatc cccgcaccgg cgcctgcgag aggcagcaac
     4741 cgttggtcgc ctgccggcaa gacgcggagg tagccccatt gtccggactg cgagtacggc
     4801 aaccgctgat tgctgtacac gtagtcaccc ggtaaccgat agggtcctcc tgccgagggc
     4861 acaaacgcgt ccagcacttc cgagccggag tattcgacca cgctgatctg gtcggcgccc
     4921 cgcatgtatg gctcgatagg ccattcatgt ttttcaacgc tgaacatgcc gttctgctca
     4981 ttgttggccc cgatcacatg aatacgcacg ggatcgcccg catgtgattc gatgatcggc
     5041 gtggcaggat cctctggttt atcggcctta caaggctgga acaccttgcc caaggagcac
     5101 ccctgctctt cgcgatactt gtagggttcg gaccggtagt tgacgccggt caatcccgcg
     5161 acgttttgca catagggcat gaaacttgtc ccgatgatgt tgtcctcgtc ctggaagaac
     5221 aaggccacgt cgcgatagtt cgccttcgac tcgttgcccg gaatgctgcg gtcgatgatc
     5281 acatccgccg cccaggcatt cttgttagac agatcggcac ccgtcttggg atcacgatat
     5341 ttcgacccct ttggaccaac caccacggct ccgaacaagc cgttgcgcgg gttcgtcatg
     5401 acgttgcccc agtcccagac cagcgaagtc gtctcgccgt tgaacggatc cgcataatat
     5461 gtgtaggtcc gttccccgcc gggcgcgatc gtctgatcgc cggggttgtt gccgacgttg
     5521 gcgcccattg actccttcgg gtcgaacact aacccgatgg ccgagaacga cgccttgccc
     5581 tctttcatct tgtttttcaa cttcactttc acgcaatcac cgacgttcac gcgcagggtg
     5641 agaggcatcg gatgggatcc gcccgagacc ttcgcagcat cttcttccaa ggcatagatc
     5701 ttggcgtcgg gattgctgat ctggattttc cgctcgaagt ccacttctat ggcttcgggc
     5761 gccttcggat taaacgtcat cgcaggataa tccattgcca cgacgttgaa gttcttcacg
     5821 ggtgcgtccg acggacacac cggcagcgcc ttcggtatct cgcccttcgc cgagaacccg
     5881 gccggcagct ttttcagatc gggctgttct ttatcgtaga ctcggagaag gccccaagcg
     5941 ccctccgaga atttcgagga ccgtccgttg aagtggatgt agtccccggc gagatggcgc
     6001 ggaccacctg cctccggcac gaccaggtcg taccgttcgg caataccgat gtggatcgaa
     6061 tttttccggt tggcatcgga agcatagcgc tctgtccaga acgtgtgccc ggccaatgtc
     6121 caggtcatcg tctcgttcat gagcgtatgc aggagccgga agaccatcgt gtccccggta
     6181 tacgctcgca acagcggcgt atacgggtcc ccgtgaatct gactattgaa cgcctgcgag
     6241 acatccggat tggtcgccaa ccgctgagcg attgggccgg ctctaaaatt cagaccgctg
     6301 cctgtggtat gcgttccgcc gttcaggaac ggcatcggag tcatatagat tttttccggc
     6361 atcatgaagg agaccgtctt gccggcctcc agtgccacct ccaccggctg tccgggagga
     6421 ttgcccgcgg tcacgatgtt gacggtgtgc ggcacggtgt cgtgcacctg caccatcaat
     6481 tcgcggaagc tgttgttcac gccatggcct acaggctcgt tggtatggat gtcggcgata
     6541 ggcccactcc ggacgggctt gccggttttc ggatcatgat aggtcgaccc gaccggctcg
     6601 gcgatgaacg tgccgaaccc accgtgcggc catgtcgtcg ctccgaacgc atgatcgtgc
     6661 cagaacaccg tcccgacgtc cgcatcgacc cagaaccgct gccggacgaa ttccaccgtc
     6721 acgatgtcgt tcgccgggtg atcatttttc aatcccttcg ccaaggtgat ggtgttgccg
     6781 ttgattgcct tgatccgaga gatttcgttg cctttcacat tgtcggcgcc aacgagaatc
     6841 aaggtgccga catgatactg cttcgcattc ttgaccgaga gactattcga gcctttcttt
     6901 gcggccgccg tcaacacagt gttcatgggc gccgggaggc cctttttatt tttcttctcg
     6961 agcatcgtga acggccggac cgactgctca tacgaaaagc cggtgatcac tccgtcggat
     7021 gcctggttat cgaactgcaa gaaatgccag tgggtgttga tcttcgacga ctggaagttc
     7081 gtgtagtcgt cgtcatccca ctcgctggtc agcgtccaat ccacgcaatc gtagatgttg
     7141 ccgcggacga ccaacggata cttcaagtcg tcattggccc gaatggcggc ttcttcttca
     7201 tggagcacgt aaatcaagcc gttcggatcg accacaggcg gttccttgcc ctgtgccttg
     7261 gcgattttga tcggcagctt gatgaaatgc acgttatagc tcttccgtcc cgcattgacg
     7321 gggcagaggc tccaattgcc ctgctcgcca ggcgcagcct gttcgacgct ttcttcaccg
     7381 ttcgcttcgc gcctgatcat ttccaaccag ggcgccccaa cgtgattcgg cgagaacatg
     7441 acacgcttgc cgaagtgcgg cgtcaggtgg ggccaagcca ctttcccggt cagcggttcg
     7501 aacatgatcg gatgccgctt gcctggatgg gtggatttgt acttcggatt gtcgatcgtg
     7561 ctttcttttt cgctcatcgc cacattgccg ttccaggtcc aatccagaac cgtggcgtca
     7621 tacgagacaa tctgaccttt ttcgtcgtcc ttgtgaccgg gcttgcccgc cgtgggcatc
     7681 tgcatctcga cccaatcctt gatcgtgatg gtggccggat tgcctttcca gttgctctta
     7741 cccttctcga tgatcttgaa ctgcgtcccg aaccaatcga ccgtttggcc gattagttta
     7801 tcggaggaga tcggcgcctt gatgcggcct ttgcgatccg gcagctctcg cagatccggc
     7861 atcacatcgt tccgcatatc gccctgctga atcgtgttgt acacgcgcca ataaccccac
     7921 atccccgcca catagtggtg ggcgacgtga caatggaaga ggaagtctcc ggccagttgc
     7981 tggcacagac cggatccgca ctccgtctca agatcgagcg cttccgaggg tccaatgact
     8041 tccacgtcga cgcggtccgt cttggcccga atcaccggat acttcacggg accgttcgtg
     8101 ctcgtggccc agagattcat gtcatcgatc gcccgggggc tcctcggcca ccggatggtg
     8161 ccgccgtggg gatgatgact atggaacacc tccgatccgc catgaaccaa ccggaacttt
     8221 gcgggatcgc ccatgtaaga acgtggaatt gtcggcgacg gatcgccgaa cgtataggag
     8281 ctataaccca tcgattcgtc ttcgaacccg aagtactcgt gctgcacgtg catgttatta
     8341 atgccgaatg gctcgctgcg atagttgatc gagcggccac cgggacgata gacatccgtg
     8401 agcgggtcgc gctgaggcag gaagtcgccc ttcttgttca gaggacggaa ggcttcatcg
     8461 ccgacctcat gatagatcag cacaaactca cggaagtccg ggccggtgcc gttcttgatg
     8521 atcgtttgcc agccgctcgt ggccggcgtg ggatcgccgg tacccagcgg ctcgaggtag
     8581 ctcgatccac gcggctcgac gacgaatgcg ccaaagagtc ccatgaccgt caactcacgg
     8641 tcgttgctgt aggtatggaa ttgccggaca ccctcttggg tgctcggatg gatgtaccat
     8701 tcatactcgc cggtcttgtc tttcgcgaca atcgtatcag gattggtcgt cgtggcagcc
     8761 gcccctgtcg cactgacgac catggcggag ccgtgaatat gaaggctgac ctcttcgcca
     8821 ccgtccaact tgttgctgag cttgatcttg aggcagtcgc cctggttacc acgaatcaca
     8881 agcggctgaa tccactgcgc ctggacaccg ggaatcacgg cacccggatc gtgaccttct
     8941 ttgtcgcgtg cttccttatt ggtgttttcc tccgcacgaa ccttgtcgat atcctcatcc
     9001 aggacataca tgtagcccgg ataaaaatcg agccactggt tgagcgtgat ctcgacgttg
     9061 accgcggaga cgttgtattg cttcaccggt gctgtggcgg gacacttacc gccacccgtc
     9121 atggcgacgg gctcgacacg cgcatccgag acgagcagca tatcctgccc gccggcaccg
     9181 tattgatgca tcatggccga ggtgttgtac ataccggtgt tgacgccctg catctgcgga
     9241 tcctgggcca tatgctccat gatgcgctga tgctgctgct cgaccattgc ggcacgctcg
     9301 cccttgccgg acatcgtatc ctcgatgatc gtctgccctt tgagctgctc agcccaggcg
     9361 ggaagctgat gattcatggc cgcatgggac gatgggtcct cggcggatgc gaagggggcc
     9421 cagagaaggg ccgctgctgc caacacgccg agcgtgcagg tgggtcgata gacccgtgtg
     9481 aaatggagaa acatggactg gcctccttac gaatatatga gtggactgac acaatgcgag
     9541 tgtaattgcc tggaaggacc tccgtataaa gaccgaaata aggaccgatt gactaaaaat
     9601 gcaccgatat ttcccgcgtc aaacgccgtc tgggccgcga gggaattgga aagatactta
     9661 agttccctag ggctgtcaat ccctttgatc gcactagggt tctccattgc ccggcacccg
     9721 cttgccacgg cgaactccga gcgccgcaaa cgctgatctg aggctttcgc cccatggtga
     9781 gaggtcttct tcttctctcg ttccatgcgt gtttatgcga taatctctgc catgcttctc
     9841 aagcggtggc tcgtcggcaa tcccatcaag acggcccatg cagcgcacga acggctttcc
     9901 aaaagtctcg ctctcgcaat attttcatcg aattccattt cctccgtcgc ctatgccacg
     9961 gaagaaattc tcctggtctt aatccttgcc ggcaccgcgg cgttggcatg gtccatcccc
    10021 gtcagcctcg ccattttgtt tctcgtcatc gtcctgacca tttcgtatcg ccagatcatt
    10081 tacgaatatc cggaaggcgg gggagcctat atcgtggccc ggtcgaatct tggcgagatg
    10141 ccggcgctga tcgctgccgc ggcgctcatg atcgattatg tcctcacggt agctgtgagc
    10201 gtagcggccg gagtcgcagc gattacttcg gccattccgg agctgtttgc gcatcgggag
    10261 gctctgggac tcatctcgat cgtgttcatc gtggtcgtga atctccgggg cacccgggaa
    10321 tccgggaaat tcttcgcgat cccgacgtat tttgcgatcg gggctttggg actgatggtg
    10381 ttgatgggga cggttcggtc tttcaccgat ggggtcgcga tgctccctct acccagtccg
    10441 gcgggaggtg accttgaaaa tctctctctc tttctgatcc tccgctcttt tgctgccggc
    10501 tgcgcagccg tcaccgggat ggaagtgatc tccaacggcg tcaaagcctt tcgctctccg
    10561 gaatcggaaa atgccgcgag gaccatggtc tggatgtcgg tcatcctcgg atcgctcttc
    10621 atgggcatca gctggatggc ctaccattac ggcatcatgg ccaaggaaga cgagacggta
    10681 gtctcacaac tggcccgtct caccttcggc acgggggtga cgtactatac gatccaagtg
    10741 ggcaccatga tgctgctggt gctcgcagcc aacagtgctt ttgccggctt tccgcatctg
    10801 gcctcgattc tcgcgcggga cgggtttatg cctcatcaga tggccacgtt cggcgatcgc
    10861 ctggtatttt cgaacggcat tatcatcctc gggttcttcg cgtgtctgct cctagtcgtc
    10921 tttcagggag acacccacgc gctgattccc ttgtatgcca tcggagtgtt cgtctcgttc
    10981 acgctttcgc aggccggaat ggttcgccgt tggttggtga agaagggact tcactggcgg
    11041 aagaaactca tcgtgaacgg cgtaggtgcc gtcacaacgg ggatcgccac ggtcatcatc
    11101 gcggccacga aattcatgca aggtgcatgg atcgtgttcc tcctcgtcgc agcgctcatc
    11161 atgatattca gaggcattcg ccgccattac aaagcggtcg cggagcaggt catgctgact
    11221 cgtgacgcac gtccgcctcg ccctcgtcgt aatctcatca tcattcccat cggtgcggtg
    11281 aacaaggcgg tggttcgagc tgtcgattac gcccgcagtc gaggaggcga ggtgcgggcc
    11341 gtgctcatcg acgtggacaa ggaggaaacg gcggtcgttg aaatcaaatg ggcccagtgg
    11401 ggctgtggcg tgccattgat tgtgctgcct tcaccctacc gctcgatcct gggcgcactg
    11461 ctggactacg tcgaagaaaa tctcgacaaa gatcccgact gttggattac cgtggtgctg
    11521 cccgagattc tgcccgcccg ctggtggcaa aacatcttgc ataatcaacg ggccctcctg
    11581 ctgaaaggtg ccctgttgtt caaggatcgc gtcgtcttaa cggatgtccc cttccacctg
    11641 acgaggtgac catgaagcca cagtatcgat tacaacggac gattactgct cggatgcgaa
    11701 ggctgatcat cctgctccaa ggtttcgtgt tcgtgctggt gaccggtatg ctcgcggacc
    11761 acgcccaggc cttcaaaatc actgaaccgg cggcagggac caaattggga gctggacaaa
    11821 ccgtcacggc tcacgtggat ctcggaaacg acacgggcat catcaaagtc cgctattatt
    11881 ggtatggaga gcaggacgac acactcgtcg aacaagatga tgcgaccgcg atgggatcca
    11941 tcatcgctcc ggtggcgctt gtcgggctgt cgggtcacga tccggcgttc gggggcaaat
    12001 tagtcgtccc gaaagaaggg atcggcccga tgcgactcct tgccgtggcg gaaatctcac
    12061 ggggtcgcct gggctcgcgc tccgtgttcg atgaaattct agtccacgtc gagccgaagg
    12121 ccgcgctgac gacgatcgac ttcgaaaccg acaaaccgct gcagctcgga cgctccgggc
    12181 agtcgtcagc ttttggacac gtggattcga tgggcaaaat cttcgagctt cccgtggtcg
    12241 gcgactttgc cgacggagtc acccggcgca tcagcgcgcc ctcgacggga acaagttatg
    12301 attcctcgaa ccctcaggtc atcaaagtgc tgccaagcgg tctgctgcag attgtgggca
    12361 atgggaaaac cgtcctcacc gtgaagaatc gggacaagca ggcgcaactc gatgtggatg
    12421 tcaacgtgaa cgaggagccg aatgaaccac cggtcgccga cgccggtgac ccgaagacgg
    12481 tcaaagcggg aaccaaggtc aagttgagcg ggctcaggag ccgggatgcc gagggcgaag
    12541 cactgtatta tgcctggagc caggtacgcg gaagtaaagt cgcactgctg gacgtcaatg
    12601 ggcccgaagc gacgtttcaa gctccgcaag tgtccgaagc aagaacctac cggtttaaat
    12661 taagagtcac ggataggaaa ggcgctgata gcgtcccggc atttgtggat gtgacggtcg
    12721 aaccgtaaac tgcccttgcg tgcggcagcc ttgccagcaa ttacgtcgat gcgtttcccc
    12781 atctccgctg gatcgcctca tagtcatttc gatcggaatt cctgctcgaa gccgggaagg
    12841 atcgctttga agaacgcgtt ctggatgagg ccgatcacaa tctggacgat ttctgtccgc
    12901 ggccgctgcg cactgccgga catatcgacc ttggtggcga cttcatcgcg aggaacattc
    12961 tcgagcaacg tcgagaggtc ttctatcgcc ccttcacgga ccttctcgag aggattcttg
    13021 tggcgatcct gcgcttcgtc atagacgttc aattttctca ctaacggctt gacatacccg
    13081 cgaatcctgc cgtcctccaa catgacttcg gaatagcagg aaaatgtccc gccgaccgcg
    13141 tcgaactttc catgggcgcg caacagattg ttcatcgccc ggatcggcgt gccggcaatc
    13201 ttcactcgta attcgaagtc gggagactca tgctccggac gcagaatacc ggtgacactt
    13261 gtctcgccgt ttcccatgaa tttcccccgc aattgcaccg aagcggcccc ttcttgcaat
    13321 cgattgctgt agtgttggag ggcggcctcc gcatcggcca ggaacacgcg atactcaggt
    13381 ttggcagctt gattgacaaa cccgaactca ctgttgacga tggtgatttc ctccactcgg
    13441 acgagcgtgt cctcactcgc atgaacctgt tgggccgtgc cgactgtcgc tctcgcccgt
    13501 gccacctccg ctccttgcgt ctgtctggca tggacgtaat ccgcatgcag cccgtcaatc
    13561 ctgagccgct tcaagctgac cagttttttt gccggtgcat attcaacgga cccttcagcc
    13621 gacatgctgc ctccactgag ctgaagattg tatcgagccg acaccggcaa gagggcagtc
    13681 aacgggacgt tctctaacaa gatgtcggcc ttcacggcga cgtaaggttc ggccagaaaa
    13741 tcggcatgcc cgtctactcg aagtttccct tcgtcgaata cctgcccttc aagttgcagc
    13801 tcggacgggt acgtctggtc gccggactga acattgcgga tattggtcgc atagacctcc
    13861 agatggtcaa aatgaagcgg ccgctcgggc ttcgcctcat cgatgtacgt caactcggca
    13921 ttggccatcc tcacttgatt gattttgaac ggatagatgc tctccaccgc atcctgccac
    13981 ccccggtctc tcaccggcct ctcatccccg acttcctttc tcgccagcct tctggtgatg
    14041 tggaatctcg gccgttccat gtattggtca ttcacgacac gtccggaaag cagcgccttc
    14101 caatggaccc tcgcggtcca gcgtgcaatc gtggccatcg gaggatcggg ttcatccgtg
    14161 cgaaccaatt caacatcctc gagatccaca gagaatccaa tcggatgaaa atccagggcg
    14221 ccgatgcgga ccgtgtagcc cttgagactg cggttcatcc tctgctcgac ataagcgcgc
    14281 agcggctcat ccacgaaggc tgaggcgaca acgaccacaa ccaccactag accacatccc
    14341 agagcgagct gtacccagcg cttcgacatg tcctccagtc taacaaggcc atcgaaccag
    14401 ccggagctag ggaaagaagc agtcacacac gctatgggcc acatggctct cgatcgtcca
    14461 ggcgaattca ggcctttcac agtggtcagc cgtgacgcgg cccataggtg tggcgggctt
    14521 gtcgaagctc tgtctggtgc gcttcgagga gtcgaccgta ggacgagtca aggacggtat
    14581 gtcgatgctt ggagatgttg cgttcagcct tcgcaatggc ctgctcaagg tgcctcacca
    14641 gccgcgcccc ggagccggtc agccatttga gacgatcgcg tgcatactga aggtcgcgga
    14701 gggaatatcg gacggattcg atttcctcca gacagcgaaa ccgcgcccgc agcaggtccc
    14761 cgctggacaa tcccgtctct cgccattgcg ctaggcctga cctcgtttcg gcccatgacg
    14821 acaaacgttc gaataactgc gcacccatgg tgaatgtgaa cgtcgcgtgc agaatcccgc
    14881 gaaccggccg caggctcctg cgccacggcg aatagaagat ctgctggttg tggtcgtcgc
    14941 ggtacagcac gtgctttcgt aacaacaaat ttaagtgatg atgactgttt tcgtggatca
    15001 gatcatcaat gaggtcaaga ttgtcccgat gaaaacaatt cacaaacgag agaccaggtc
    15061 gatggcggta actgaagctg accacaccgc gagccttgag cggcacgata cgtgatgtca
    15121 ataattccag cacacgatgg gcttccggcc aggccaggcg gatggctttc caagcccgtg
    15181 caatcctggc cacttggcgc ggtgccgtcg ccgacagtgc ggtcggctcc tgagtttttc
    15241 cgtagtgcag cgtcggtccg acggtcagtg gtccgtacgg cgtgttggcc gctaccaaca
    15301 actctgtcag cgagcagtga tactcaatcc ggtttccccg acgcctgcag agaggtcggg
    15361 accggccgag tcttagcgtg atgtcgttcg gagtcactat ccaagtcggt cgtcgccggc
    15421 gtcctcggag gtcccgtgcg acaacccgag gcacgagcag tgctatgccc tgtgttccga
    15481 tcggcacagt acccgctcgt tccccccgct cgaagcagtg gtctagaccg gccggcactt
    15541 cccatgtctg acgaccttcc cgcgccaacc aggccatcgg ccattgcgga tcgaaaaaaa
    15601 gcgactcctc cgtcacctca cgtgcttgcc tcacgcatag tcgatgcagt cgtccctcaa
    15661 gcccaaaggc ccgggcctgc ctttgaggga acaggtcgtc cgtataacta ttttcaaaaa
    15721 acttttgttc acattcatca aacagctgct gggcaaagag ttgcgggttc cgttcctttc
    15781 gaatctgtgc caacacgtct agaaaataga ccagatcgtt gagcgcttcg atccacccca
    15841 ccactttcca attggaatag ctagatccct cgaacgcttg ggtcaaaaac agaaagtatt
    15901 cgatcggaag accgagcccg ctggccaacc cgatatagtg ccgttcgaga tcgtggcaca
    15961 gatctctgag tagcctgcgc atggaacttc gaaactcatg ttggacgcga cggagctggg
    16021 ccgtatcgag cagcgacatg atctactctc ttttgaaaat gtgcgaccct accggtacgc
    16081 gtccccccgg caaggagcag gatgaggagc tggcgggaag agaccggagg tcatcgagag
    16141 tgtcctatcg aaccttgagg aaatgcgtga tggttcgata gaggtccacg gtcgcttgct
    16201 ctttgctgag cagggccgaa gcgcccgcat ccagcacggc attgagtgtt tgggctgaat
    16261 gttggacgga caatccaatc accacagcat tcggcgcata gttcttgatc cgcttcgtcg
    16321 catcgatccc gtttagcttc ggaaggttga tgtccatgac gacaacgtcc ggctgtgtgc
    16381 tcttcgccga ttccaccgcc tcaaggccgt cggccgcctc cccgatgatt tccaagtcgg
    16441 agtatgtctc gagaatgctt cgcagaccct gccggaccat ggcatgatca tccaccagca
    16501 acacacgcca acgcgacggc acggcggctc tggaaggatc ggcaactccc acagcggttc
    16561 tgtgaacatg cagtttccga ctggcatccg ccggcatcga cgctaacccc agctcaacgg
    16621 tggtcccctc gccgggcagc gagtgaattc ggcaccatcc ggacatcgcc tccatgcgtt
    16681 cctgaatgct gaacagacca aaatgttcac ccgaaggcag gtactgcact gcattgagat
    16741 cgaaacccgc gcctgagtcc tgcaccgtaa tcaaccaggt atcccgatct tgcttgttta
    16801 ccgtcaccac ggccgaggaa acgcctgcgt gttttacaac gttggtgagc aattcccgta
    16861 gggcttggaa cagcaaatcg gactgatgtt cgctgaaacc cggctgctca tcggtgagaa
    16921 cgcgcacatc cacggtgagc ccttgctgcg ccatctgctc ggccaaccaa tgaatggcgg
    16981 ggatgagccc cagatcctgc aagaccgacg ggctgagctg ggccatcagc gtccgcgcat
    17041 attggaggga ctgctgcaac agtgtatcca tctcggtgag cgcggaccga acctccggcg
    17101 ccctgtcgtt gagacgttgg agagcctgac ccaacttaat tcgaattaag gccaagagtt
    17161 gcgcgagata atcatgcaga tctccggcca accggcggcg ttcccgttgc tccgtcagat
    17221 tcagctgtga ggccagcgca cggagtcgct cttgagattt gacgagttct cccgtccgtt
    17281 cccgcaccct cgcttcaagc tcagtgttcc aacgctgcac ctgcagttcc gaactcttgc
    17341 gccccgagat gtccctgaag acgagcacgg caccaatcaa ccgtccgtca tgatcccgga
    17401 tgggtgccgc gctgtcgtcg atggggactt ctgtgccatc agggcgcacg agcacagtat
    17461 ggtttgccaa gcccacgacg aggccatctc gtatgacccg ttcaacaggg ttgtcgacag
    17521 cctgccgagt gtcttcattc acaatagtga acacctctgc caaccggcgt cccatgatct
    17581 cggcagcggg catgcccaac aggtcggcgg cgatcgggtt aataaatgtg acgaccgcgg
    17641 acgcatcggt ggcgacgaca gcatcgccga tgctctccag tgtcacccgc caacgctctt
    17701 cgctttcatg aagcgcccgt tgggtgcgtt tctgttccga gatatcgcgc gcgatttttg
    17761 aggcaccgat gacgttgccg tcgtcatcgg tcaaaggcga gaccgtgatc gacacatcga
    17821 aaagccgtcc atcttttttg catctgatcg tttcataatg gtccacacgc tcgccgcgcc
    17881 ggagctttga cacgatcgcg gcttcttccg aatgacggtc cgtggggata atccgcagaa
    17941 tggaggatcc gatcatttct tcggcggtat agccgaacat gcgctcggcg cctcggttcc
    18001 aactcgtgat cgtgcccgtg aggtctttgc tcacgattgc gtcgtctgag gaagccacga
    18061 tcgaggagag cagtaccgcc tgccgggcag ccttatattg gtcggacacg tcccggaaca
    18121 cgagcaccac gccgagcagc cggccatctg catccttgat tggggccgca atgtcatcga
    18181 tgggaacttc tgttccatcc cgccgcaata ggaccgtgtg attggccaag ccggcgaccg
    18241 taccttcgcg caggacccgc tcgacaggag agggaaccgc ttgtcgcgtc tctgcattca
    18301 ctatggtgaa aaacttatca agcggctgcc ctatgatttc ttcgatggaa caacccatca
    18361 gccgctccgc cacaggattg agataggaga ttcgggattc ggtgtcggtg gccaacacgg
    18421 catctccgat gctttccaag gtcgtggcca gccgcacctg cgcttcagcg atgacctgct
    18481 tcgcctgctt acgctcggta atgtcgcggg cgattttgga ggctccgatg atggtcccgt
    18541 tgccgtcgcg cacaggagac acagtcagat cgatatgaat aagacggcca tcttttcgcc
    18601 gccgcacggt ttcgaaattc tcgatgcgat cgcccttgcg aagacgctca aggattgaag
    18661 cttcctcggg atgaaactcc ggcggaatga tcagcaggat tgactggccg attgcttcct
    18721 gctccgtata tccaaagata tgctcggcag ccttgttcca tgtctggatg atgccggata
    18781 gatttttcgt aataatcgcg tcgtgcgatg aatcgacgac ggcggctagg taggcggctg
    18841 cttcaccggc gctgtgagtt gacccaggca ttgtgtgtcc gtgggggcaa aaattttttt
    18901 attctcgccc ccttggaccg gaaaagcaag aatgcggccc tccctatcgc tcgggatatg
    18961 acggattcgc accccgtttg gccgtgattc ttctttgaac cgagccgaag ccttgtagtt
    19021 ccattcagcg ggtgagctcc ggccaacgat tctatgcaac gcgaggcatg ccagtcattg
    19081 gtgcccgttg tcgggacaag ttgtccaaat gttttttcaa cggagcagat ggaccgcaga
    19141 aaaacccgga aaatggttgg tttccgcgcc ggcactgaca cattgatggt tcactcaagg
    19201 ctatgagctc ggcgcgtgtt ttgcaatttc tctagcgtag gaggtgtcta tggctacccg
    19261 gtggtatgac gcatttaagg ctcggtcgct ctggcacctg cttaaggagg ctgcttttga
    19321 ttggagccat gaccgggctc cccgcctcgg cgccgctttg gcttattaca ctgtcttttc
    19381 gatcgttccg cttctcatcg tgatcatcgc cctcatcggc ttgttcttcg gcgaggacgc
    19441 agcgcagtca gcgattatgg accaaatctc taatctcgtc ggagagcaga gtgccgcggc
    19501 cattaaagac atgatccaac gggccgagca gcccagcacc ggagtggttg caaccgtcgt
    19561 cgccgtggtc actttgctgt tcggtgcgtc cggcgtgttc ggacagcttc aagatgcgct
    19621 caatacggtg tggggcgttg agccgaagga gggacgcggg gtctggggct ttatcaagga
    19681 tcgtttcctc tcgttcgtgg cggtcgtggg aacgggattt ttgctcctcg tgtcgctggt
    19741 gcttagcagc gcattggccg ccctcggaaa atggttcagc ggtgtcctgc ccttgccgga
    19801 ggtggtcctt caggcgctca attttatggt gtccttcgtc gtgattacag gcctctttgc
    19861 attaatcttc aaggtgctgc ccgatgcccg ggtagcctgg cgcgatgtgt gggtaggcgc
    19921 cgcgctgacc gcggctctgt ttaccatcgg caaattcgcg attgggctgt acttgggaaa
    19981 gagcaatgtg gcgtcggcct atggcgcagc cggctccctc gtcatcgtcc ttgtgtgggt
    20041 gtactactct gcgcaaattc tgttatacgg tgcagagttc acccaggtat acgctaacag
    20101 gctcggcgaa cggattgtcc ccgcccctga tgcgcaagtg gccaacccgc agaaggcctc
    20161 caacttggag ggtaacaaga gagaggctgg tcgcgccgcc taagcattct ccagtacaac
    20221 agtctatgaa tcctcagtcg gccacctctt caagcgcctc ccccgtgtcg tctccctacc
    20281 atctattgac cttcctggtc ggtgccatga agaggcgcgt gtggtatcga agagaactgc
    20341 gggtctactg ctacccgctc gagggtagcc cacctcttcc ccgtcccgcc atctttgaaa
    20401 ggaatcgctt tgatgacctc aagtactacg aacgcacaga caaatatcaa ccatcgcccg
    20461 aggtgtatcg caaaatggcg ctcgagcgaa agacacaagg attccatctg tacacgttcg
    20521 tgcaagagcg gcgattgctg cactatgggt ggctcatcga gcgccaaaca cgtggcgaag
    20581 acgactgggt acagcaagcc tatttccctc cgcctgacac atcagttctc ttcgatcact
    20641 ttacccaccc gtcagcgagg gggaaagggt tgtacttcca ggcactctgc agattgttgc
    20701 atgacgcccg ggatttgtca ggcgctcgcc aggcctatgt gacggtgttc ggatctaatg
    20761 cgccttcccg tcatgtgatt gagaaagtgg gctttcacca cgaaggcagt ttgtataaag
    20821 agagaaaact attctggtcg acccgctatt cggtaggcac acacgacgac ttcagaaccg
    20881 ccttgctctg actgccaccc ctactcccgc ctttggcgac gcgtccggga cgactccttc
    20941 acatcctcgc gccgtgcccg aaaatttgcc agggacggaa cggtaggatc ggaagccgat
    21001 gacggcggag ccagccggtt cacggacaac accagctggg cgcgacgaat ggcatcgagc
    21061 aatgcttgac gttcggtttc gagtcgtttc gagcgtgggg agcgattcac gaagttttct
    21121 gctgcatctg tcacgatgtg aaacgcttcc cagacatcct gcgatggtcg tcgcttcatc
    21181 gatcatgtcc tcgtcactag cctaccggag agtcgctgga tgaccactga aatatcccct
    21241 gggtcccaga acgtggcgtt ttatttgagc tcagagaggg gtcgaacaaa ggcatgacaa
    21301 ccatcctcgt ttcatcgaaa tacagggatg tgcctagtgg ccttatctcg gattgagcac
    21361 tagcttgaag agaaaggttg aaatctggtg cctgtcggca gggattgtag ggggcagccc
    21421 gtcatgcccg acgaaggcag aagcagcgat atccatctct caaggaggtt tccatgatac
    21481 acgcgcactg tatgaagacc gtgatgggcg gtctctctct tgttcttgtc ttgtcggcct
    21541 gttcttccgg atccaagtcg aagaccacga gcgctcctcc ctcgggggac atgtcgcggt
    21601 cggacgacat gaatcgg
//
LOCUS       DASSCQ010000106         5830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_790307, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000106 DASSCQ010000000
VERSION     DASSCQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790307"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3131
                     /locus_tag="VFH05_07695"
     CDS             <1..3131
                     /locus_tag="VFH05_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678134.1"
                     /translation="SNPVIVNIKDWVEMFVPAQGKPGHTDKEKDQILAYDSTVWDWKW
                     DGNIARGERESTAQNPKYSWAAKWDDSTRPAILFDPTTGKMAWPLFKPHFGKRVPFSA
                     NHSGAPWLEPIHQDANGERTSEPAKPGEQGRWSLCPDNANQKFYNLHFLRMPITLAKQ
                     QGKEPPIVDKDGLIYVLHEEERLTRGNDDLKLPAVIRANVYDCVDLVLTSEWDDDDYT
                     NFQSSKINIHPHFFQFDTGNSDGVISGFEYEMSVRPFTMWGKPKKHGLPAPMVAKIVG
                     GAKAGTSSIKIQLAPGATPFHVNTEVMVGVDCLEKGNDPTASLPRDKSCSEVARIKDI
                     KGDQVTFFKPLKHNHPASDLVSPEFVRYRWWVDVDMGTVFWHDHAFGATTWPHGGFGV
                     TIVEPFGSTYHNPKDGKLVYSGPVADIHSNEPIGAGVSGSFRELMVSIHDTVPHTVNV
                     IEAGNPPGQPVEVALEAGKTVSFQMPEKILNAPNKYINGGTHTTGSGFNFRAEPFAQR
                     LSNNPDTSKLFSSAIHGDPDTPLLRAYTGDTMVFRLLHQLMNESHVWTIAGHTFLTER
                     YAADANRKNSIHVGIAERYDLVTKAGGFQGMPGDYIHFNGRSSHFAEGGWGIVRVLDK
                     ETSDLKPLPKGTNPLGIPATPNEVCPADAPVKSFTVVALDRPLKLHPKAPEAIEVDFE
                     RKIELTVPEGKIFALEEEATTVAGNVMPNPLTLRANLGDCIKVNLKNKMKASRASFFA
                     PGLAFDPKDSQGLNVGNNGGDQTIGPGESRNYTYYAHPSNKETTSLVWDGGNIVVNPR
                     NGLYGAIIIGPKGSQYRDPVTGADLSQKNAWRADVVVDASLPENVGKRNYRDVALFFQ
                     DEDNIIGTSFMPYVQNVAGLTSVNYRAEPYKYREEQGCSLGKIFQPCVVDKPEDPATP
                     LIEAHAGDPIRIHVIGANSEQNGMFGVEGHEWPIEPYMPGADMISVVEYAGSEVLDVF
                     IQGGAGGPYRQTGDFVWSNNRLPYAQSGQWGYLRVLPTGDSRIQPLGAASAGAKRVDM
                     QPAPQATPTAMR"
     gene            3770..4879
                     /locus_tag="VFH05_07700"
     CDS             3770..4879
                     /locus_tag="VFH05_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6678135.1"
                     /translation="MKTYKRHILVLIVLVVLFGGGYLVLGAGFWSRGLPEGLIQVNGR
                     IEGDHLTVASKFPGRIQELLVREGANVTMGQVMVRIDDVQARSRVDQARHGWEATEAK
                     VQSAHTALAVLNLEVPLAIESAQSHVDEAKAKLEKSKAVEQEARLDEQRLRSLLPEQA
                     VAQQQYEQALARWNVAKSEVQVAKATLAKTMKELAQAELGWKRIHAQEDEVAALERQR
                     DQLEAALNEAESVLADLTIRAPANGTVTTRMVDLGEVVTPGTPLFEIVDLDRLYLKVY
                     VPEIQIGKIRLDLSARIHTDAFPDRPVDATVQYIASKAEFTPKEVQTPDERVKLIFPV
                     KLYLKDNPDHRFTPGLPADAIIRWKENVAWVPPKR"
     gene            4975..>5830
                     /locus_tag="VFH05_07705"
     CDS             4975..>5830
                     /locus_tag="VFH05_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6678136.1"
                     /translation="MNDVQLTIKQGEIYGLIGPDGSGKSSLMKAIAGVLSYEGGVVEV
                     FGTSVDSEFAAEQVKPQIGFLPQGLGLNLYPELSVEENIDFFARLRLVPEQKLTERKT
                     RLLAVTRLDKFRHRAMKHLSGGMKQKLGLICTLIHEPQLAILDEPTTGVDPVSRRDFW
                     AILAELIHTKGMTALVSTAYMDEAARFHRLSFFSQGKVLAAGTPSDVLRLVPGSILSF
                     EATPQMEAIARIKKQYGQVEAFGSRLHVFTNEIDHAEAVKHLKSSLGDIEANRMQVRE
                     PELEDVFVA"
BASE COUNT         1344 a         1557 c         1761 g         1168 t
ORIGIN      
        1 agtccaatcc ggtcattgtg aacatcaagg attgggtcga gatgttcgtg cctgcgcagg
       61 gcaagccggg ccatacggac aaggagaagg atcaaatcct cgcctatgac tcgacggtat
      121 gggattggaa gtgggatggc aatatcgccc gaggcgagcg agagagcacc gcacagaatc
      181 cgaagtattc atgggccgcc aagtgggacg acagcacccg acctgcgatt ctctttgatc
      241 ccaccacggg caagatggcc tggccattgt tcaagccgca cttcggcaag cgtgtgccct
      301 tctcggcgaa ccatagcggc gcgccctggc tggaaccgat tcaccaggat gcgaacggcg
      361 aacggaccag cgagccggcc aagccaggtg agcaaggccg ttggagtctc tgcccggaca
      421 atgccaatca aaagttttat aaccttcact tcctgcggat gccgatcacc ttggcgaaac
      481 agcaggggaa agagccgccc atcgtcgaca aagacgggct catttacgtg ctccatgaag
      541 aagagcgtct gacgagaggc aatgacgatc tcaagcttcc ggcggtcatc cgcgccaatg
      601 tctacgattg tgtcgacttg gttctgacga gcgaatggga cgacgacgat tacaccaatt
      661 ttcagtcgtc caagatcaac atccatcccc acttcttcca attcgacaca gggaactcgg
      721 atggcgtgat ctcggggttc gaatacgaaa tgtccgtgcg tccgttcacc atgtggggca
      781 agcccaagaa acacggctta ccagcgccga tggtggccaa gatcgtcggc ggagccaagg
      841 cgggaaccag ctccatcaag attcaactgg cacccggcgc cacgccgttc catgtgaaca
      901 ccgaagtcat ggtcggtgtg gactgtttgg aaaagggaaa cgatcccacg gcctccctgc
      961 cacgagataa gagctgctcc gaagtcgctc gcatcaaaga catcaagggc gatcaggtca
     1021 catttttcaa gccgctcaag cacaaccatc cggccagcga cctcgtgtcg ccggagttcg
     1081 tgcgctaccg ttggtgggtg gacgtggata tggggacagt gttctggcac gaccacgcgt
     1141 ttggcgcgac gacctggccg catggcgggt tcggcgtgac gatcgtggag ccgttcggct
     1201 ctacgtatca taatccaaag gacggcaagc tggtgtatag cggtccggtt gcagacattc
     1261 actcgaatga gccgattgga gccggtgtca gcggaagttt ccgcgaactg atggtgtcga
     1321 tccacgacac ggtgccgcac accgtcaacg tcatcgaggc cggtaatcct cccgggcagc
     1381 cagtcgaggt ggcgctggaa gcgggaaaga cggtgtcgtt ccagatgccc gaaaagatct
     1441 tgaacgcgcc caacaaatac atcaacggcg ggacacatac gacgggcagc gggttcaatt
     1501 tccgtgcgga acccttcgct cagcgcctgt ccaataatcc ggatacgtcg aagttgttca
     1561 gcagcgcaat ccatggtgat cccgataccc cgttgttacg ggcgtatacc ggtgacacga
     1621 tggtgttccg tctgctgcat cagttgatga acgaatcgca tgtgtggacg atcgccggcc
     1681 ataccttcct cacggagcgc tatgcggcgg atgccaaccg caagaactcg attcacgtcg
     1741 ggatcgccga gcggtatgat ctcgtgacga aggcgggcgg gttccaaggt atgccgggcg
     1801 actacatcca ctttaatggt cgaagctcac acttcgccga aggcggatgg ggcatcgtgc
     1861 gcgtactgga taaggaaacg tccgacctca agccgctgcc gaagggcacg aatccgctcg
     1921 gcattccggc gactcccaat gaagtctgtc ctgccgatgc accggtcaag agcttcaccg
     1981 tcgtggcgtt agatcgcccg ctgaagctgc atccaaaagc gcctgaggca atcgaagttg
     2041 atttcgagcg caaaatcgag ctgaccgtgc cggagggcaa gatcttcgcg cttgaagagg
     2101 aagcgacgac ggtggcgggc aatgtcatgc cgaatccgct gacactgcgc gcgaacctgg
     2161 gcgactgcat caaagtcaat ctcaagaata agatgaaggc cagccgggca tcgttctttg
     2221 cgcccggatt agccttcgat cccaaagaca gccagggctt gaacgtcggc aacaacggcg
     2281 gcgatcagac catcggacct ggtgagagcc ggaactacac ctactacgcg catccgtcga
     2341 acaaggaaac gacgtcgttg gtgtgggacg gcggcaacat cgtcgtcaat ccgcgcaacg
     2401 gattgtatgg cgccattatc atcggcccga aaggatcgca gtatcgtgat ccggtgacgg
     2461 gggccgatct gtcgcagaag aatgcctgga gagccgatgt cgtcgtcgat gccagtttgc
     2521 cggaaaatgt cggcaagcgg aattatcgag acgtggcctt gttcttccag gacgaagaca
     2581 acatcatcgg gacgtcgttc atgccctatg tgcagaacgt ggctggcctg acgtcggtga
     2641 attatcgtgc cgaaccgtac aagtatcgtg aagagcaagg ctgctcgctg gggaagattt
     2701 tccagccctg tgtggtggac aagcctgagg acccggcgac gcctctgatc gaggctcatg
     2761 ccggcgatcc gatccgcatt cacgtcatcg gggcgaatag cgagcaaaac ggaatgttcg
     2821 gcgtggaggg ccacgagtgg ccgattgagc cctatatgcc gggcgccgac atgatcagtg
     2881 tggtggagta cgccggttcg gaggtgctcg acgttttcat ccagggcgga gcgggaggtc
     2941 cgtatcgtca gactggagat ttcgtgtggt cgaacaatcg gctgccgtat gcgcagtcag
     3001 ggcaatgggg ctacctgcgt gtgttgccaa ccggcgattc acggatccaa ccgttgggtg
     3061 cagccagcgc gggcgccaag cgggtggaca tgcagccggc gccgcaggcg acgccaaccg
     3121 ccatgagata accatgcgag agatgttcta acggctacag gggagctgcc gccaatcggg
     3181 agctcccctg ttgtttctgc aaggcctgcc ggcggcgagc ccaggagaag tgaatgcttc
     3241 cgattcgacg aatcatcctc gactacggtc gcagttgcga acgtcttctt tcgagtcggt
     3301 cactcgggac tccacttacg gttgatgaaa tgcaactcat ccgctattac ctgagagaga
     3361 tggagcacag attcgattca gataccggta accgtcactc ccaatgctca gagggctcat
     3421 gtcatggaac acaacgagat caataagtgg cgtcgatggg agttggcggt caatgtgctg
     3481 ctggtcgctg tggtactctt cgtgctgggc cgggcagtct gggcctagag aagtccgtcg
     3541 acgctgttcg gttctctcca tggtcttttt atgatgagta gtctctacgg acaatcggac
     3601 cgcctcaagc tgcctcaaaa tacgacctgt ctcagttaag atcgctattc ccgatctttc
     3661 cccgcagttc tctcgttgca ctccagtaaa aaaaaggatt aaggtcatcc tcccggacgg
     3721 agatcggatt gaaccggagc agggcaggta tccatcctcg tgatttgaga tgaagactta
     3781 taaacgtcat attcttgtac tgattgtgct ggttgtcttg tttggcgggg gctatttggt
     3841 cctcggcgcc ggattctgga gccgcggctt accggaagga ttgatccagg tcaacggtcg
     3901 catcgaaggc gatcacctta ccgttgcgag taagtttccc gggcgcatcc aggaattgct
     3961 ggtacgtgaa ggagccaacg tcaccatggg gcaagtcatg gttcgcatcg atgacgtgca
     4021 agcgcgcagc agagtggatc aagcccggca tgggtgggag gcaactgaag cgaaagtaca
     4081 gtcggcgcat acggctctgg ctgtgctgaa tctggaagtg cctttggcaa ttgaatcggc
     4141 ccaatctcac gttgatgagg cgaaggcaaa gttggagaaa agcaaggcgg tcgaacagga
     4201 ggcccgtctg gacgagcagc gattacgcag cctgcttccc gagcaggcag tggcgcaaca
     4261 acagtatgaa caagcgcttg cgcgatggaa cgtggcgaag agcgaagtgc aggtcgcgaa
     4321 ggcgacactg gcgaagacga tgaaggagtt ggcgcaagct gaactgggat ggaaacggat
     4381 tcatgcgcag gaggatgaag tggcagcgct ggaaaggcag cgagaccagc tcgaagcggc
     4441 tttgaatgaa gcggagagtg tgttggccga cctgacgatt cgtgcgccgg cgaacggtac
     4501 cgtgaccacg cgaatggtgg atcttggaga agtcgtgacc cctgggacgc cactttttga
     4561 gatcgtggat ttggatcggc tctatctcaa ggtctacgtg cccgagattc aaatcgggaa
     4621 gatccggttg gatttgtccg cgcgtatcca tacggatgcg tttccggacc ggccggtcga
     4681 tgcaacggtc caatatattg cctccaaagc cgaattcacg ccaaaagaag tccaaactcc
     4741 agacgaacgc gtgaagctca tcttccccgt caagctttac ctgaaagaca atcctgatca
     4801 ccgattcact ccgggtctgc cagccgatgc catcattcgt tggaaagaga atgtagcctg
     4861 ggtgccgccc aagcgatgaa caccacggcc aacgaatcag tgcctaggcc cgccacgggt
     4921 cccgccgttg tgaatgttga acacttcttg aaatgttaca aaaagcaggt ggcggtgaat
     4981 gacgtccaac ttaccatcaa acaaggcgag atctacggcc tgattggtcc ggacggctca
     5041 ggcaagagca gcttgatgaa agccatcgcg ggagtgttgt cgtatgaggg cggggtcgtt
     5101 gaggtgtttg gaacctctgt tgattcagag tttgccgcag agcaagtgaa gccgcaaatt
     5161 gggttcctgc cccaaggact tggcctgaat ctgtatcctg agctctcggt ggaagagaac
     5221 atcgatttct ttgcccggct gagattagtt cccgaacaga agttaactga gcgaaagacg
     5281 cgattgttgg ccgtcacaag actcgacaaa tttcgccacc gggccatgaa gcacttgtcc
     5341 ggaggcatga agcagaagct cgggcttatc tgcacattga tccacgagcc gcaactcgcg
     5401 atccttgacg agccgacgac gggagtggat cctgtgtccc gccgggactt ctgggccatc
     5461 ctagcagaac tgattcacac gaaaggcatg accgcattgg tgtccaccgc ctatatggac
     5521 gaggctgcgc gattccatcg cttgtcgttt ttctctcagg gaaaagtcct ggcggcggga
     5581 acaccgagtg acgtgctacg gctcgttccc ggatcgatcc tctcctttga agccacgcct
     5641 caaatggaag cgatcgcacg gattaaaaaa caatacggtc aagtggaggc gtttggatcc
     5701 aggctgcatg tgttcacgaa cgagattgac catgcggaag ccgtgaagca tctcaaatca
     5761 agcctaggtg atatcgaggc gaacaggatg caagtacgtg agccggaact cgaggatgtt
     5821 tttgtcgccc
//
LOCUS       DASSCQ010000107         6037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_847615, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000107 DASSCQ010000000
VERSION     DASSCQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847615"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VFH05_07710"
     CDS             complement(<1..982)
                     /locus_tag="VFH05_07710"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6678137.1"
                     /translation="MRRGAACYGCGVVLMTGVGWLIASQTFLNTATAMDSGGIIVGTV
                     QGQDLRRVDQAMVQVRDQEGNLVVQTVTNQAGEFRVVVPQSGTYSISAVRDTDRSEYV
                     VLRIGAEQPAPVSLTLTATREIALEIVSPLPAIQYKASSETYQLSRKEIETLPKGNNN
                     DVHEVLLTVPSVVYGGLKQTHIRQDHANQQFRIDGIPIPDTVSGSFADIVPPRAWERA
                     DIILGGMEAQYGNKTALVVDITSKSGTTPGFGSAQVFGGSNQTVNPSFEYGGAIGEKV
                     RFYLLNSYATTSRGIEPPTLGKTNHHNQSEKNNTYLRGDYQHDNHNNFSWI"
     gene            complement(1073..1387)
                     /locus_tag="VFH05_07715"
     CDS             complement(1073..1387)
                     /locus_tag="VFH05_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678138.1"
                     /translation="MSPRVPSHSQALSLSLILLILVFAPFAAAQDIHHELAAADADGH
                     EHSDTDLCQWVQHHTSGSVDLGGPICSAWQSTGQYDVPLPSILFSIDSTAVGPSRAPP
                     HS"
     gene            1537..2595
                     /locus_tag="VFH05_07720"
     CDS             1537..2595
                     /locus_tag="VFH05_07720"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6678139.1"
                     /translation="MNRPLDNQHVIEIKALPSPRAIKTKLPITDDVAALVVETREAIR
                     RILHGQDRDRLLVIVGPCSIHDPEAAFEYAIKLKPLSDAVRDRLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIATGMELARTTLLKINQSGMPCATELLDPISPQYIADLISW
                     TAIGARTTESQTHREMASGVSMPVGFKNGTEGSLHVAVNAMTSARTPHHFVGINADGQ
                     TSIIKTMGNPDRHIVLRGGGGKTNYDAEHVAKAEAAVAGEGIARSIMIDCSHDNSSKD
                     HTRQALVAREVLRQFRDGRQSIMGLMLESNLNPGKQTWKQGVALAHGVSITDACLGWD
                     DTAALLNELAESIVARPA"
     gene            2736..3506
                     /locus_tag="VFH05_07725"
     CDS             2736..3506
                     /locus_tag="VFH05_07725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET6678140.1"
                     /translation="MLIDTHTHLDDTRFDGDRDTMIDRARQAGIEAFVTIGCDLATSR
                     SAVALADQHPDVFASIGVHPHEVQYVDDAWYSEFRRLAQHRKVVAYGEIGLDYHYNHS
                     SPQSQRQRFREQIQLARELRLPLIIHTREAQEDTITILKEEKAAEIGGVFHCFSGDAW
                     LAKDALDLGFYLSFSGILTFQNAAMLRDIAKQAPLDRVLVETDCPYLTPVPHRGKRNE
                     PAFVQHVAKQLAAIRPDLSYEDIEHTTSANAKRLFKIP"
     gene            complement(3493..4017)
                     /locus_tag="VFH05_07730"
     CDS             complement(3493..4017)
                     /locus_tag="VFH05_07730"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET6678141.1"
                     /translation="MHVIIDGYNLLAVLRPLGGANLYSDAAREALMRVLAEYRHRKGH
                     ALTVVFDGWQRGQPIENREHRAGVHVIYSKRGERADQVIQRLAREYGDQCAVVSSDHE
                     IVNSAQAHGALVISAPAFADKLHRSRSSETSAPYKELDTGEDSVERRGPEKKGNPRKL
                     PKSLRRRSRQLKGF"
     gene            4152..5435
                     /locus_tag="VFH05_07735"
     CDS             4152..5435
                     /locus_tag="VFH05_07735"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET6678142.1"
                     /translation="MPATVSRVAALILVLGLLSTEGFLPDGGWLGPAHAALQDQRRDH
                     EKASKRSRNSNISLSPNTIPLAKIQNLRTAFLPDSLRLVLDLDRKTQVKKHRPAQQDG
                     VVVEIPHAALTKSAQVKIAQGVLEGRFVVKQTAGDSVTIALPSGSFETYSLLSLTNPP
                     RLVVDVIPSGLQPSGGSITDPTDSSMLSVTPPQPVAPRVKSYKTIVIDPGHGGRDPGA
                     HGQRGTEEKDITLKVGLKLRDLLRKDPNVRVLMTRERDVFVDLEDRARYANGNEADLF
                     ISIHVNSHPQRSVRGIEIYHFGEAKDQRALELAARENGTPLSSTGVGWEYLVADLLTS
                     KKIEASLELAWNTKEAMIAHLNGDYALVDHGVKTAPFYVLRFTSMPSILAEIAYISNA
                     AEEDLLRTGVFTTRVAESLHEGVNAFLAASKTPAR"
     gene            5463..>6037
                     /gene="truA"
                     /locus_tag="VFH05_07740"
     CDS             5463..>6037
                     /gene="truA"
                     /locus_tag="VFH05_07740"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET6678143.1"
                     /translation="MPTVRLILEYDGSRYAGWQRQPDQPTIQESVETALFQLTQETVA
                     VIGAGRTDSGVHAIGQVASFRIERDWTPREWMRGLNARLPEDIAVRSAERVSNDFHAR
                     YAARGKLYEYRILNRPQRPALDRAYVWHVHKPLDHRAMEQAAIHLVGSHDFSSFEGTL
                     TDNEDPVCHLRQLSLTRREDRLTIHAYADRFL"
BASE COUNT         1318 a         1828 c         1621 g         1270 t
ORIGIN      
        1 aaatccaact gaagttattg tggttgtcgt gctgataatc gccgcgcaga taggtattgt
       61 tcttttcgct ctgattgtga tgattggtct tgcccagcgt cggcggctcg atgccgcggc
      121 tcgtcgtggc gtaactgttc aataaataaa accggacttt ttcgccgatg gctcccccgt
      181 attcaaagga tggattgacc gtctgattcg atccgccgaa aacctgggcg gagccgaagc
      241 cgggagtcgt cccgctcttg ctggtgatgt ccacgacgag tgccgtctta tttccgtatt
      301 gcgcttccat gcctcccaga atgatgtctg cccgttccca tgcacgaggg ggaacaatgt
      361 ctgcaaagct tccgctgacc gtatccggga tagggattcc gtcgatgcgg aattgctgat
      421 tggcatggtc ctgtcgaatg tgcgtctgtt tgaggccgcc gtagacgacg ctgggcaccg
      481 tgagcaacac ctcgtgaacg tcgttgttgt tcccttttgg caaggtctcg atttcttttc
      541 ggctcagctg atatgtttcg ctcgaggcct tgtattgaat agcgggaagc ggtgacacaa
      601 tctccagcgc aatctctctc gtcgccgtaa gggtcagcga cacaggcgct ggctgctcag
      661 cgccaatcct caagacaaca tactcactcc gatcggtatc ccggactgca ctgatcgaat
      721 aggtgccgga ctgcggcacc acgactcgaa actctcccgc ctggttggtg accgtttgga
      781 ccaccagatt cccttcctgg tcacgcacct gaaccatggc ttgatctact cgtcgtaggt
      841 cttgaccctg cactgtgccg acaataatcc cgcctgaatc catggctgtg gcggtgttga
      901 ggaatgtttg actggcgatc agccaaccga ctcccgtcat caacaccaca ccgcagccat
      961 aacacgccgc tccccgtcgc atacccacct ccgcaatcac gagcgatgac gcagatgctg
     1021 gtcataggag ggcggtgatc gcccaagcta tgacgtcgca ataattcgcc ggttaggaat
     1081 gaggcggtgc gcgggagggg ccgacagcgg tcgaatcgat cgagaagaga atcgagggaa
     1141 gaggaacgtc atactgaccc gtagattgcc acgcggagca gatcggcccg ccgagatcga
     1201 ccgagccgga cgtgtgatgc tgcacccatt gacacagatc ggtatccgaa tgctcgtgcc
     1261 catctgcatc agctgccgcc agctcatgat gaatgtcctg tgctgcagcg aacggcgcga
     1321 acaccagtat caagaggata agactgagag aaagcgcttg agagtgggag ggcacccttg
     1381 gagacatgca gcctaactat cacaaggtcg taaatactgt caaaatcagg ttattctcca
     1441 tgagcccgta tcttttcaac aacttagatc ggatcatgta gacttgtctt gaacatttcg
     1501 ctatgctact gcacttcgac aactgaaagg ccggccatga atcgaccgct cgacaaccaa
     1561 cacgtcatag aaatcaaggc attgccctcc ccacgggcca tcaagaccaa acttccgatc
     1621 accgacgatg tcgccgcgct ggttgtggaa acacgggaag caatccgtcg gatcctccac
     1681 ggtcaagatc gcgaccggct cctcgtcatc gttggtccct gctcgatcca tgatccggaa
     1741 gccgccttcg aatatgcgat aaaactcaag ccgttgtcgg acgcggttcg cgaccggctc
     1801 ctcatcgtaa tgcggaccta ttttgaaaaa ccccggacga cggtcggctg gaagggactc
     1861 atcaacgatc cgcatttgga cggcacctgc gatatcgcca ctgggatgga gcttgcccgg
     1921 acgactctcc tcaagatcaa tcaatcgggt atgccctgcg ccaccgagct gctcgatccc
     1981 attagcccac agtacatcgc cgatcttatc agttggaccg cgatcggtgc ccgcacgacg
     2041 gagagccaga cgcaccggga aatggcgagc ggagtgtcca tgcccgtcgg gttcaagaac
     2101 gggacggaag gcagtctgca cgtcgccgtg aacgccatga cctcagcccg aacccctcac
     2161 cattttgtcg ggatcaacgc tgacggccag acatcgatca tcaaaaccat gggcaacccg
     2221 gaccgccaca tcgtgctgcg cgggggtggc ggtaaaacta attatgatgc cgaacatgtc
     2281 gcgaaagcgg aagccgcggt ggccggtgaa ggcatagcac gatcgatcat gatcgactgc
     2341 tcgcatgaca attccagcaa ggaccatacg cgccaggccc tggttgctcg ggaagtgctg
     2401 agacagtttc gtgacggacg ccaatcaatc atgggcctga tgctggaaag caatctcaac
     2461 cccggaaaac agacctggaa acaaggcgtc gcactcgctc acggcgtttc cattaccgac
     2521 gcctgcctgg gctgggacga caccgccgca ctccttaatg aactcgccga aagcattgtc
     2581 gcccgacctg cctgacgcgt ttcatgaaca tgcttcgagc agccgatgcc tgtgcatgcc
     2641 ttcacgattt caggtggtca aaagccatcg ctcaacaaag ccctctaacc ggctcctcgc
     2701 cgtcccccgt ttaggtcctt tctctcgggg attgcatgct cattgatacc catacccatc
     2761 tcgacgatac ccggttcgac ggcgaccgcg acaccatgat cgaccgagcg cggcaagccg
     2821 gcatcgaagc attcgtgacg atcggttgcg atctggccac cagccggtcg gccgtcgcgc
     2881 ttgccgatca acatccggac gtctttgcat ccatcggcgt gcatccccac gaagtgcaat
     2941 acgtcgacga tgcgtggtac agcgagtttc gccgccttgc gcaacacagg aaagtcgtgg
     3001 cctatggcga aatcggactc gattatcact acaaccattc atcccctcag agccagcggc
     3061 agcggtttcg cgaacagata caacttgcac gcgagttgcg cttgcctctc atcattcata
     3121 cgagagaggc acaagaggac accatcacga tcttaaaaga ggagaaagcc gcggaaatcg
     3181 gcggggtatt tcactgtttc tcaggggatg cctggctggc taaggatgct ctcgatctgg
     3241 ggttctatct ttccttttcc gggattttga cgttccaaaa tgccgcgatg ctgcgggata
     3301 tcgccaagca ggcacctttg gatagagtgt tggtggaaac cgactgtccc tatctcacac
     3361 ccgttcccca tcgagggaaa cgaaatgaac cggcgttcgt ccagcacgtc gccaagcaac
     3421 tggccgcgat ccgtccagat ctctcgtacg aggacattga gcacaccacc agcgctaacg
     3481 caaaacgact attcaaaatc ccttgagttg tcggctgcgt cggcggaggc tcttcggaag
     3541 ttttcgggga ttgcctttct tctccggccc acgtcgttct accgaatctt caccggtatc
     3601 caactccttg taaggggctg atgtctctga tgagcgtgac ctatgcaatt tgtctgcaaa
     3661 tgccggcgcg ctgatgacca gcgcgccgtg tgcctgcgcg gaattcacga tttcgtggtc
     3721 ggagctgacg accgcgcact ggtcgccata ttctcgtgcc aaccgctgga tcacctggtc
     3781 ggcacgctcg cctctcttgg agtaaatgac gtgaactccc gcacgatgct ccctgttttc
     3841 gatcggctgt ccccgttgcc agccgtcaaa gacgaccgtc aatgcatggc ctttccggtg
     3901 ccggtattca gccaatactc gcatcagagc ctctctcgcc gcatctgagt acaaatttgc
     3961 tccacccagt ggccgaagaa cagccaggag attatagccg tcgatgatga catgcatgac
     4021 cagcagaccc tacgcccgct ccaaggaggt gtcaattcat tgcagtccgc ctcgagaaac
     4081 gtcgctcccc ttgacaacca ccagaacatc tgagagatac atatatctcc cgcagccttc
     4141 tgtggaaacc catgcccgca actgtctctc gcgtcgccgc cttgatcctc gtgctgggac
     4201 ttctcagcac ggagggcttc ctgccagacg gcggctggct tggtcctgca cacgcagcct
     4261 tgcaggacca acggcgcgat catgagaaag catccaagcg atctcggaac tccaacatca
     4321 gcctctctcc caacacgatc ccgctcgcga aaatccagaa tctgagaacc gcttttttgc
     4381 cggattcgct ccggctggtg ctcgatcttg accgcaaaac gcaggtaaaa aaacaccgtc
     4441 cagctcaaca ggacggagtg gttgtcgaga tcccgcacgc cgccttgacc aaatcggcgc
     4501 aggtgaaaat tgcgcaagga gtcctcgagg gacgctttgt cgtcaaacaa accgccggtg
     4561 actccgttac cattgcactg ccgagcggct cgttcgaaac ctatagcttg ttatcattga
     4621 ccaaccctcc tcggctcgtg gttgacgtca ttccctctgg gctgcaaccg tccggcggat
     4681 ccattacgga ccccacagac tcatctatgc tttctgtcac cccaccgcaa ccggttgcac
     4741 cgcgtgttaa atcctataag acgatcgtga ttgatcccgg acacggcgga cgagatcccg
     4801 gcgcgcatgg acaacgcggt accgaagaaa aggacatcac gctcaaggtg gggttgaagc
     4861 tacgcgacct gttgcgcaag gacccgaatg ttcgtgtgct gatgacccgg gagcgggacg
     4921 tgttcgttga tttggaagac cgtgcgcgat atgccaacgg caacgaagcc gacctgttca
     4981 tctccatcca cgtcaactcg catccgcaac gatcggtccg tggcattgag atctatcatt
     5041 tcggcgaagc gaaggaccag cgtgctctcg agttggctgc tcgtgaaaac ggcactcccc
     5101 tcagcagtac tggagtggga tgggaatatc tcgtggccga tcttctgacg agcaaaaaaa
     5161 ttgaagcctc ccttgagctg gcctggaata ccaaagaagc gatgatcgct cacttgaacg
     5221 gcgattacgc gctggtggat catggggtga aaaccgcacc cttctacgtg ctccgcttca
     5281 ccagtatgcc gagcattttg gcggaaatcg cctacatctc caacgccgct gaagaggacc
     5341 tcttgaggac cggcgtcttt acgacgcgcg ttgcggaatc cctccatgag ggtgtgaacg
     5401 cctttctggc cgcttccaaa acaccggcac gctgatgcgc cgcacggccc tgctggtccg
     5461 ccatgccgac tgttaggctc atccttgaat acgacggttc acgctatgcc ggctggcagc
     5521 gtcaaccgga tcagccaacg atccaggaat ccgtagagac ggcgctgttt caattaacgc
     5581 aagagactgt cgccgtcatt ggggccggac gcaccgattc cggggtccat gccatcggtc
     5641 aggtggcgag cttccgtatc gaacgagact ggacgccgcg tgaatggatg cgaggcctca
     5701 acgcaaggct ccccgaggat attgccgtcc ggtctgcgga gcgtgtctcg aacgactttc
     5761 acgcgcgcta cgccgcccgg ggaaaacttt acgaataccg gattctgaac cggcctcaac
     5821 ggccggctct cgatcgcgcc tatgtctggc atgtgcacaa gcccctcgat catcgcgcca
     5881 tggaacaggc ggcgattcat ctggtgggct cgcacgattt ttcttccttc gaaggcacct
     5941 tgaccgacaa cgaggatccg gtctgccacc ttcggcagct gtctttgacg cggcgagagg
     6001 accggctcac gattcacgcc tacgcagacc gttttct
//
LOCUS       DASSCQ010000108        15008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_198070, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000108 DASSCQ010000000
VERSION     DASSCQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198070"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..945
                     /locus_tag="VFH05_07745"
     CDS             151..945
                     /locus_tag="VFH05_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET6678144.1"
                     /translation="MKSALSILFGVAAVAFVAAPVTSFAGGTIAGKVTYAGKPEKKEF
                     SFAKFPNPKFCPKNPHKELIDGDKRFMPTIEVGKDGGLKNAVVAVVDVEDKAFMDGYK
                     GTEVVAEFCEFLPYSGVVVNNKSFKVENHDADPDDPKSTQGVLHNPHSFTVKGSTSAT
                     GFNIGLAKKGDKLDKPVVFRGGAEKDGFYRLQCDQHEFMQSFFLPVSSPHFAVVKEDG
                     SFEIKDVPPGKHKVVAWHPFVGKGKKTEFEVEVADGGTANLKAELK"
     gene            1280..2272
                     /locus_tag="VFH05_07750"
     CDS             1280..2272
                     /locus_tag="VFH05_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET6678145.1"
                     /translation="MGVPRELSLAEIFQLGYYWETKILLTAVRLDLFSALEGKPKSPQ
                     EVAARIDAHEPTLTLLLNALVAMSLLTKTDGGYRNTTVSGKHLVRHSPHYIGHLLLLH
                     DAEWNNWGRLEETIRSGKRLVDRHVFETDPELGSNVLAVLDRIGRQSGPEFAKRLRLD
                     GPVHLLDLGGGAGTNAIAFCEVYPGLTATVFDLPATLRLTEKTVKEAGLETRITLRAG
                     DFNRDGLGGPYDLVLMSDILHYQTFGTNQALVKKVYDHLGPDGRLIIKDRFVDETGTG
                     PAWTTAFAVHILVNTQEGACYTTAEAMQWMTKAGFVAVSEVERTAVVQGIKAAR"
     gene            2280..2993
                     /locus_tag="VFH05_07755"
     CDS             2280..2993
                     /locus_tag="VFH05_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HET6678146.1"
                     /translation="MDWLKHPGFFGTHATIGADVSQLMATFFTGLFVIGWIQARKRQS
                     DAHHWLMLGGMVAMLGFFVSYYLFRQLGVLAFEGKEGFGGSEALYRNVFIPLLTFHII
                     LVIIGLVMAVYMIVLGFRAQAIEQGRRILSGRILQTTWGKVGTIFGILCAVVAVYMVY
                     LVAADRFRMGRLAVWVGLLALIAIVFAVEMAIQRIWPDGGARHRTLGRFTMIIYCILF
                     VTGTTTYTMLYILYPGKIG"
     gene            3134..3622
                     /locus_tag="VFH05_07760"
     CDS             3134..3622
                     /locus_tag="VFH05_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HET6678147.1"
                     /translation="MLTCGCGRWMHTDGIEERAYDDGAAQWFIRSECRGCGFKVGMDV
                     PVGQHMFIDRLMWTDEALHRLERMPPYLAPVVRADVERDVRARGEKVVTYDSVLRVRT
                     GEVIEWEAEAERRLENVPAPVRAMARIELERTAADRGMARITVALMEEVKAKYFGMGK
                     AS"
     gene            3659..4702
                     /locus_tag="VFH05_07765"
     CDS             3659..4702
                     /locus_tag="VFH05_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678148.1"
                     /translation="MLHRMALRVLPSLTLAVTEPAVRKQKRLVMLVPGLVAFAVYRLA
                     KILMPLSETSVLLTVSGMVGLITAVLAYRAGRATSWVEIIRADGRKLLAWMAGWIGFV
                     YAVQLSLLVLALLWLVGYDYLQHPDGPAMMAMIISCTAVARDAFEIGHIRYLTMMGRP
                     CPTFPNGVALRSLLQSHAVALRPWVGIGLLAGALGASFGALIENHQAAALLQVAAITI
                     LGGAITLCAYFAGLTPSGRWMEGLMETKAAELLKYWWWPGLAFASTYYLVVTGVVIFL
                     LKQPGVPTSYAVCGGSLVAGVMALYGYYLGYRRQVEEQQGLSLSPALLRCPFVMGILT
                     KASGSPVAGASKG"
     gene            4708..6573
                     /locus_tag="VFH05_07770"
     CDS             4708..6573
                     /locus_tag="VFH05_07770"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6678149.1"
                     /translation="MLSIIRSFHAIVAILALTCGLGFFGASAPSALAEEAAADLYYKI
                     PGTPSGPAAPSPTETVYSRVGSYDSRLAVWFVTQQHTYFGGFVLALPLFCALLEFLGL
                     LTNKPSLSLRYDGLARDLAKVALLALSVTAVVGSLMLGMFVYLYPSFMRYMGGTFKAF
                     MPAYAVVFLGESLLLILYYYSWNRMAEPGLKWIHAAIGILTNIFGTALLLLANSWAAF
                     MMSPAGVDAQGRFLGNGWHLLHSALWNPLNVHRFLADIMSGGAVVLAYACYRFFTSKT
                     EGERAYFDWVGYVFLFVTVCALLPMPIAGYWLMRSVYAFRQSMGVTMMGGLLTWLFVV
                     QALLIGALLLGVNYYIWQSMARIKGGERYQTHYQFLVGALTLALFIWLTPHTVLMSAG
                     EVKAMGGAQHPVVGNYGVMSAKNGAVNVMILITALSYLYYRRANRTMTTSWVRTGNLV
                     IGTLFAVGLLNVIWLSVYGFYLPAKVRVGLSSPQAVTTLTVIVVSLLINRAMLKGSIV
                     HGPVQWGKIPIRGMVVLFALAAAFTWVMGLMGYIRSSGRLSWHVNELMADVSPWAFTP
                     ELGFAAKMVTLNMIVFWSAVLALFWMCQLGQQQELKEEWFEESSQTLASVPPAET"
     gene            6599..7246
                     /locus_tag="VFH05_07775"
     CDS             6599..7246
                     /locus_tag="VFH05_07775"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HET6678150.1"
                     /translation="MKRISKISAMLMAIALFVPASGSGSAQGQRLVLEDFQTKEADGF
                     PTSWDHESQRSHSKGREAYKVQAEDGSNYLSAKDAGQRIKKKKIDWDPKAYPVLTWRW
                     RLNKAAEGTEPLAAVYASLDTDLMFIPVFTKYIWSATKPVGTLIEGGMFSGSEIVVQS
                     GTTPVGQWVEEQVNVYEDFKRIHQHEPAAKAWGISIVAGSGVEIDFGPMVATSDK"
     gene            7268..7621
                     /locus_tag="VFH05_07780"
     CDS             7268..7621
                     /locus_tag="VFH05_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678151.1"
                     /translation="MSPVHRWLDIAFGLAVMGTVGAMIGIIMGGGLMPIAIMVGLGMG
                     GVIGFLGGRRFLVSILIGSVLGGALAWLVAGTERISVGAGTGAAMGGFLGVQISMLLD
                     VRAARKASSQPVDQP"
     gene            7638..8558
                     /locus_tag="VFH05_07785"
     CDS             7638..8558
                     /locus_tag="VFH05_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET6678152.1"
                     /translation="MDNKGVLIGSIIFVFGSFILMIGMMLWESNKAYKMKELARTVQT
                     DSRPAASTLPAQDYSMYKTKMGDEGREMVQIPEGPFTMGSQDGDPDETPEHQVFLKGF
                     FLDRKEVTQEEYMRFAKMTKRGTPKIEVFEDDQSKLLKPEFAAMSVSWDDALAYCRWA
                     GKRLPTEAEWEKAGRGEGKRRYPWGDKFITNGANVDGSEDGYKYLAPPGSFDAGRSPY
                     GLYDMTGNVAEWVNDTYEENYYKKSPYRDPKGPEGIGDLRVVRGGSWRETEANARLSK
                     RFAAKHWRTDITIGIRCASDLDQGGAAPTS"
     gene            8566..9465
                     /locus_tag="VFH05_07790"
     CDS             8566..9465
                     /locus_tag="VFH05_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6678153.1"
                     /translation="MLETKFKVALLLIVLAFAALPVMGILRGTTTTPYEDAAPAPDES
                     DLSQHESSSQEVPVHDDMVTIPAGPFLRGTASGGFDEQPQRSIFLETFSIDRYEVTNH
                     HYQQFAAATGHRKAGPPSRYAKSIGRMRGTNQPIIYVSWEDAADYCRWKGKRLPTEAE
                     WEKAMRGPDGRLWPWGDHEKPNGANWARVQDGYEVTAPVGTFQTDRSPFGVMDGAGNV
                     MEWVADWYQEAYYKESPDKNPPGPEYGTYRVMRGGAYTTAGVDLRITSRSKMVPDFRD
                     ETIGFRCAASGIFSGNSGARGKS"
     gene            9697..9828
                     /locus_tag="VFH05_07795"
     CDS             9697..9828
                     /locus_tag="VFH05_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678154.1"
                     /translation="MATATATAPDSEIKIKIGKMIFYITCAIGLWFFYWFAGIQCPC"
     gene            9897..10019
                     /locus_tag="VFH05_07800"
     CDS             9897..10019
                     /locus_tag="VFH05_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678155.1"
                     /translation="MGQTIVYILISTVICVAYFTLVDHYLMDMQGLDYWYLFRK"
     gene            10098..12014
                     /locus_tag="VFH05_07805"
     CDS             10098..12014
                     /locus_tag="VFH05_07805"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6678156.1"
                     /translation="MGPVTAKKVLSIMALCAMVTLLVLPVVVSLPSPAAGEDAAAVDG
                     AKKDGDKVEKGRDVYYKTEGIVVGAPAPKTTDGPKDYPRYNFESRVLLWFANQQHLYY
                     GSFVLAVPIFCMVIEFMGVVSKDKALAKRYDQLAYDFVKISLTAYSLTAILGGILIFT
                     FLTLYPAFFGYLSSIFRPVMHIYALMFVAESGTLYIYYYGWDKMKEGFLKWIHLSMSV
                     VLNIIGTLLMFLANSWIGFMMSPAGVDEQGRFLGNIWHVIHTALWNPLNVHRILGNMA
                     FGGGVVAAYAAYKFLASKTDEDRAHYDWMGYIAMALGVAFLIPLPFAGYWLMREVYAY
                     RQQMGITLMGGLLAWLFIIQATMIGILFLSTNYYLWQAMGRMRGAEKYQRYIKYLVFL
                     LVCGFLVFITPHTMVMSPAELKAMGGQQHPVLGNYGVMSAKNGGINVIITTTVLSFVW
                     YMRGNKVSTVSWSKFGNIFMGCFFFFAYVNIIWLAIYGYYIPANVRVGLSVPQVATTL
                     SCLFFMFGLNSVMMKGAKQMGPIEWGKISARSQYALIMLATAFTWMMGLMGYIRSSVR
                     LFWHVNEIMRDNSPWAYTHTVGFAANMISFNVLFFWISILFVFWLGSLTAKKVPIGEK
                     VGVPGGAPQPAGSH"
     gene            12092..13405
                     /locus_tag="VFH05_07810"
     CDS             12092..13405
                     /locus_tag="VFH05_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6678157.1"
                     /translation="MGNLINEALSMGWVVLALLAGLLIYFQFSISDPAAKKRATFKTF
                     IGIIAAILMFIAIANYKNNFYGENRLLPVSLVMITVTTFVMAIYFTNLAALLKIGGFM
                     FFVAAFLSGYGNWLPQVEGGFPPKEEKLDFSSMSPQQLADEGEKIIFGGIGQNKVQGA
                     IGKGQCPLCHAFHAGMLGERAPNLLGLPERAGKERLEDPKYSKGNPAKREYAEKESCP
                     GCGTAENGQEYIAESHACPSCYVVSGYGVKGTNDKESPMPKIHKPPISLSLAELAAVD
                     TWLYVREGREAPSFEEITKSYEKFIPEAERPKQQEDKPAGAASALLADGSEPVDQIFA
                     KAQCVVCHTIPGIPGATGTQGPKLEEATNAPQRIKDPAYKGTAHSTPEYIMESVVAPS
                     TYVVKGFPDNLMPKIFGQKLSAGALKKIVDYLSQVKAGAPPPKIS"
     gene            13445..14482
                     /locus_tag="VFH05_07815"
     CDS             13445..14482
                     /locus_tag="VFH05_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6678158.1"
                     /translation="MKALMSVGALVGVVGLLLLVGMIFDVVPSNTVRLVEGYMPMQML
                     FELTLFVAGFAGLSYMMSSMGMPLPRFWQGIGFWAFILLYLKYRVYPPIPFSVRAMYG
                     TVSLVAVFMWVSSNEEDWKKFKQPILNVLDAQSGGNKVLRYAYLILLPILIGGFSYNA
                     MKPKSEEPIELRTVHPAPPASTKVHGKTYVLQTSQNPYRVNPEGKYDQEFTNANIVEQ
                     GMGRLMKPNANPWDEKSTGYLKYVREGGEIFFQNCHFCHGDNLNGRGLHAFAFNPIPA
                     NFTDPGTIAQLQETFIFWRVAKGGIGLPNEGFPWASVMPPWEQHLTVDEIWKVILFEY
                     WHTGYYPRTWD"
     gene            14535..>15008
                     /locus_tag="VFH05_07820"
     CDS             14535..>15008
                     /locus_tag="VFH05_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6678159.1"
                     /translation="MMNSSSRKAGVVAVAAFGIILASGSVGVPLIGAEDMPEGFKKGE
                     LAPEPSAEMIEAGKRVYFTKCVWCHGVDGAGDGPGADRLWPRPRNFNQGTFKIRHTAS
                     GELPLFDSRKPVPGQNDLFETVTHGLPGSAMPSWEGILTEEQRLQVLSFVTTQLVK"
BASE COUNT         3165 a         3904 c         4521 g         3418 t
ORIGIN      
        1 tctacgacca gcctcgccag ccaagtcgtc aatcgagtac ccagggaggg cagcgttctg
       61 acgttcctct cgtaaccgga atgaagacgg gggacagcga gctaggaatc attattgtcg
      121 gggtttcgtt aatcacaagg aggcagtaac atgaagagtg cgttatccat cctatttggc
      181 gtggcagccg tcgccttcgt cgcggcacct gtcacctcct ttgcgggcgg taccattgcc
      241 ggcaaagtca catatgccgg gaaaccggaa aagaaggagt tttccttcgc caagttcccg
      301 aacccgaaat tctgtccgaa gaacccccat aaggagctga tcgacggaga caagcggttc
      361 atgccgacca tcgaagtggg caaagatggc ggcttgaaga atgctgttgt cgctgttgtc
      421 gatgtcgaag acaaggcctt catggacggc tataagggca cggaagttgt cgctgaattc
      481 tgcgagttct tgccgtattc aggcgtggtc gtcaacaaca agtcgttcaa ggtcgaaaac
      541 catgatgcgg atcccgacga tccgaagtcg acccagggtg tgttgcacaa tcctcacagc
      601 ttcacggtga agggttcgac ttcggcgacc ggtttcaata tcggtctcgc caagaagggc
      661 gataagctgg acaagccggt ggtgttccgt ggcggcgccg aaaaggacgg tttctatcgt
      721 ctgcagtgcg accagcacga gttcatgcag tcgttcttcc tcccggtctc cagcccgcat
      781 tttgctgtcg tgaaggaaga tggttccttc gaaatcaagg atgtacctcc gggcaagcac
      841 aaggttgtag cctggcatcc gtttgtcggc aaggggaaga agaccgaatt tgaagttgaa
      901 gtggcggatg gtggaacggc caatttgaag gctgaactca agtaagccac gcccggtttt
      961 tttgcgaggc gactcgcaat tcaaaggcag cggagtgatc cgctgccttt tgtttttctt
     1021 ctgaacacag cgcacaaccc gccttgcgtt tccgttttca aactaggtaa gatgccgtct
     1081 ttcaagaact attttggctg cacggtgttc agaaaggccg gcggtctcat cgcctgctcc
     1141 gacgcgccga gacgcgttct tccgcaagag gggcggccga agcaactcaa gggcgaaagc
     1201 gtacgctttc cggtgtattc tggccctgag gacgcgagca ctatgccgta cggacctata
     1261 aacatcctgc tggaagggaa tgggtgtgcc acgggaactg agtctcgctg aaatcttcca
     1321 gcttggttac tactgggaga ccaagattct gctgacggct gtccggctcg acctcttctc
     1381 tgcactcgaa ggcaagccca aaagcccgca ggaggtggcc gcccgaatcg acgcacacga
     1441 gccgacgctc accctgctct taaacgctct cgtggccatg tccttattga cgaagacgga
     1501 cggcggctac cgcaatacca ctgtgtcagg aaagcacctc gttcggcact cgcctcacta
     1561 catcgggcac ctgctgttgc tgcacgatgc ggaatggaac aattggggaa ggttggagga
     1621 gacgatccgt tcgggaaagc gcttggtcga ccggcatgtc ttcgaaacgg atccggaatt
     1681 gggcagcaat gtcttggcgg tgcttgatcg catcggtcgc cagagcggcc cggaatttgc
     1741 gaaacggttg cgcctcgatg ggcccgttca tctgctcgat ctcggaggcg gagcagggac
     1801 caacgctatc gctttttgcg aggtgtatcc cggactgacc gccaccgtgt tcgatcttcc
     1861 cgcgacgctg cgcctcaccg agaagaccgt caaggaggcc ggacttgaaa cccgaattac
     1921 gttacgcgca ggggatttca atcgcgatgg gctcggaggg ccttacgatc tcgtgctgat
     1981 gtccgacatc ctccactacc agacctttgg cacgaaccag gccttggtca aaaaagtcta
     2041 tgaccatctg ggacccgacg ggcgattgat catcaaagac cggtttgtgg acgaaactgg
     2101 aacgggcccc gcctggacca cggcattcgc ggtgcacatc ctcgtgaata ctcaagaagg
     2161 ggcctgttac accacggcgg aggcaatgca gtggatgacc aaggcgggat tcgttgcagt
     2221 cagtgaagta gaacggacgg ctgtggtcca aggaattaaa gctgcgaggt aaccgctcaa
     2281 tggattggtt aaagcatccg ggatttttcg ggacacacgc cacgatcggc gccgatgtca
     2341 gccagctcat ggcgactttt tttaccggtc tcttcgtgat cggctggatt caggcacgga
     2401 agcgccaatc ggatgctcat cactggctga tgttgggtgg catggtggcg atgctgggct
     2461 ttttcgtgag ttattatctt ttcaggcaac tcggcgtgtt ggcctttgaa ggaaaagaag
     2521 gatttggtgg atccgaagct ctctatcgaa atgtattcat cccgcttctc acctttcata
     2581 tcattctcgt gattattgga ttggtcatgg cggtctacat gatcgtgctg gggttccgtg
     2641 ctcaagcgat cgagcaagga agaaggattc tcagcggccg catccttcaa acgacctggg
     2701 gcaaagtcgg tacgatcttc gggatactct gtgccgtggt cgcagtgtac atggtgtact
     2761 tggtggcagc agaccgtttc agaatgggac gacttgccgt atgggtcggg cttctggctt
     2821 tgattgcaat agtttttgcg gtggaaatgg cgatccagcg tatttggccg gacggcggag
     2881 cgcgccatcg aacactcggc cgcttcacga tgatcattta ttgcatcctc tttgtcaccg
     2941 gaacgaccac gtacacgatg ttgtatatcc tctacccggg caagatcggt taacgagatg
     3001 cacgctgcgc tctactcccc cgaccatccg gtgtggttct gttacatgcg ctcgaccagc
     3061 cgatcgcttc gccgtcgatt ccccgggccg cacatttcac gaagtatgtg ttcggattgg
     3121 gaagtgtaat cccatgctga cttgcgggtg cggtcgctgg atgcacacgg atgggattga
     3181 agagcgggcc tatgacgatg gcgcggcgca atggttcatc cgttcggaat gccgggggtg
     3241 tggttttaag gtgggaatgg atgtgcctgt cggccagcac atgtttatcg atcggctgat
     3301 gtggactgac gaggcactgc atcggttgga gcgaatgccc ccctatctag cgccggttgt
     3361 tcgagcagac gtcgagcgcg atgtcagggc gcgcggggaa aaggtcgtca cctacgactc
     3421 ggtgctgcgc gttcggaccg gggaggtcat cgagtgggaa gctgaggcgg agcgtcggtt
     3481 agagaacgta ccagctccag tgagggccat ggccaggatc gagctggaac ggaccgcagc
     3541 agatcgggga atggctcgga tcaccgtcgc actcatggag gaagtgaagg cgaaatactt
     3601 cgggatggga aaagcaagct gatcaggaca gtcgtggctc aggggagcaa gggaagctat
     3661 gttgcatcga atggcgttgc gagtgttacc gagcctgact ctggcggtca cggaaccagc
     3721 cgtacgcaaa cagaagcggc tggtcatgtt ggtgccgggt ctggttgcgt ttgcggtata
     3781 tcggctggcc aagattctca tgccgctgtc tgaaacgtca gtactgctga cggtcagcgg
     3841 gatggtgggg ctcatcactg ccgtattggc ctatcgtgcc ggccgggcaa cgtcgtgggt
     3901 tgaaatcatc cgagcagatg gtcgcaagct tctcgcgtgg atggccggtt ggatcgggtt
     3961 tgtctacgcc gtccagttgt cgcttcttgt gctcgccctg ctctggctcg tgggctatga
     4021 ctatctccaa catcccgacg gtcccgccat gatggcgatg atcatctcct gtactgcggt
     4081 ggcgcgggat gcgttcgaaa tcgggcacat acgttatctg accatgatgg gacggccatg
     4141 tcccaccttt ccgaacggcg tggcattacg cagtctgctt cagtcccatg cggtggccct
     4201 ccgtccgtgg gtcggcatcg gactattggc cggagctctc ggtgcctcgt ttggtgcgtt
     4261 gattgagaac catcaggcag cggcccttct gcaggtagcg gcgatcacga tcctgggagg
     4321 tgccattaca ctgtgcgcct atttcgcggg gctgacgccg tctggcagat ggatggaggg
     4381 attgatggaa accaaggcgg ctgaattgct gaagtactgg tggtggccgg gattggcgtt
     4441 tgcctcaacc tactatctgg tcgtgactgg tgtggtgata tttctcctga agcagcccgg
     4501 cgtcccaacc tcctatgctg tctgcggcgg cagcttagtc gccggcgtga tggcgctgta
     4561 tgggtattat ctgggctatc gccggcaggt ggaagagcaa caaggactca gcctgagccc
     4621 cgctttgctt cgctgtcctt tcgtcatggg catcctcacc aaggcctcgg ggtcacctgt
     4681 tgccggtgcg tcaaaaggct aggacttatg ctgtcgatca tcagatcatt tcatgcgatt
     4741 gtcgccatac tcgcactgac gtgcggcctc ggattcttcg gagcatccgc accttccgcc
     4801 ctggctgaag aggccgccgc cgatctctac tataagatac cgggtacgcc ctcgggaccc
     4861 gccgctccct cgccgaccga aacggtgtat tcgcgcgtgg gatcgtatga cagccgtctg
     4921 gcggtgtggt tcgtcacgca gcagcatacg tacttcgggg gcttcgtgct cgccctcccg
     4981 ctgttttgtg cgttgctcga atttttgggc ctgctgacaa ataaaccgtc gctgtcgctt
     5041 cgctacgacg gcctcgcgag ggatctggcg aaagtcgcgc tgcttgcgct ctcggtgacg
     5101 gcagtcgtgg gcagtcttat gctcggcatg ttcgtctatc tctatccgag cttcatgcgc
     5161 tacatgggcg ggaccttcaa ggcgttcatg ccggcgtacg ccgtagtctt tctcggggag
     5221 tccctgctgc tcattctcta ctattacagc tggaaccgca tggctgaacc tggtttgaag
     5281 tggatccatg cggcgatcgg catcctgacc aacattttcg gtaccgcgct gctcctattg
     5341 gcaaattcct gggcggcctt catgatgtcg cccgccggcg tggatgcaca gggacggttt
     5401 ctcggcaacg gttggcattt gctgcactcc gctctttgga atccgttgaa cgtccatcgg
     5461 ttccttgccg acatcatgtc gggcggcgcg gtcgtgctgg cctatgcctg ctatcgtttt
     5521 tttacaagca agaccgaggg ggagcgtgcc tattttgact gggtgggcta cgtctttctc
     5581 tttgtgaccg tgtgtgcgct cctgccgatg ccaatcgcag gctattggtt gatgcgctcg
     5641 gtgtatgcgt tccgccaaag catgggtgtc acgatgatgg gcggtctttt gacatggctc
     5701 ttcgtcgtcc aggccctgct gatcggcgcg ctgctccttg gcgtcaatta ctacatctgg
     5761 cagagcatgg cacggatcaa gggaggcgaa cggtaccaaa cgcattacca gttcctggtg
     5821 ggggcgttga cgttagcgct gtttatctgg ttgacgccgc atacggtctt gatgtccgcg
     5881 ggagaagtga aagcaatggg cggagcgcaa catccggtgg tgggaaacta tggggtgatg
     5941 tcggcaaaga acggcgcggt caacgtcatg attctcatca cggccctcag ttatctgtac
     6001 tatcggcggg caaatcgaac gatgacgacc tcctgggtca ggaccggaaa tctcgtgatc
     6061 gggacgctct ttgcagtcgg ccttttaaat gtgatctggc tctcggtcta cgggttttat
     6121 ttgcctgcaa aagtgcgcgt cggcctttct tcgccgcagg ccgtcacaac gctcaccgtc
     6181 attgtcgtca gcttgctcat caaccgcgcc atgctcaaag gatcgatcgt ccatggtccg
     6241 gtgcaatggg gaaagatccc gattcgcggc atggtggtgc tgttcgcttt agcggcggcc
     6301 tttacgtggg tcatgggtct gatgggatac attcgctcct cggggcggtt gtcctggcac
     6361 gtgaatgagt tgatggccga tgtctcaccg tgggccttta cgcccgaact cggctttgcc
     6421 gcgaagatgg tcacactcaa catgatcgtg ttttggagtg ccgtcctggc gttgttctgg
     6481 atgtgtcaac tggggcagca acaggagttg aaggaagaat ggttcgagga gtcgtcgcaa
     6541 acattggcct cggttcctcc agcggaaacc tgaggtgtca gaaagggacg tggcgggcat
     6601 gaagagaatc agcaagatca gtgcaatgtt gatggcaatt gccctgttcg ttccggcatc
     6661 aggatcgggg tcggctcaag gacagcgact cgtcttggaa gatttccaaa ccaaggaggc
     6721 ggacgggttt ccgaccagct gggatcatga aagccagcgc agtcattcca agggtcggga
     6781 agcgtataag gtccaagcgg aggacggctc caattatctg tcggcgaaag atgcaggaca
     6841 acgaatcaag aaaaaaaaga tcgattggga tcccaaggcc tatcctgtgc tcacctggcg
     6901 ctggcggctg aataaggcgg ccgagggaac cgaaccactc gcagccgtct atgcgtcgct
     6961 ggacacggac ttgatgttca tcccggtgtt caccaaatat atttggagcg cgacgaaacc
     7021 ggtggggacc ttgattgaag gcgggatgtt cagcggctcc gaaattgtgg tccagagcgg
     7081 gaccaccccg gtggggcaat gggttgaaga acaagtgaac gtgtacgaag atttcaagag
     7141 gattcaccag catgagcctg ccgcaaaagc ctggggcatc tcaattgtgg ccggttcagg
     7201 cgtcgaaatt gattttgggc cgatggtcgc aacatcagac aagtaagtca tggatactcc
     7261 cgttgctatg agccctgttc accggtggtt ggatattgcc ttcggcctgg ccgtgatggg
     7321 caccgtggga gctatgatcg gcatcatcat ggggggcgga ctcatgccaa tcgcgatcat
     7381 ggtgggactc ggtatgggcg gagtcatcgg gtttctgggc ggacggcgct ttcttgtgag
     7441 tattttgatc ggcagcgtgt tgggcggtgc attggcgtgg ctggtggcag gaacggaacg
     7501 gatttcagtc ggtgcaggga ctggagcggc catgggcgga tttctgggcg tgcagatctc
     7561 gatgctcttg gatgtgcgtg ccgcgagaaa ggcttcgtcc cagccggttg atcagccctg
     7621 accgagggag cagagcaatg gacaacaaag gcgtgttaat aggatcgatc atcttcgtgt
     7681 tcggttcctt catcctcatg atcgggatga tgctgtggga atcgaacaaa gcctataaga
     7741 tgaaagaact cgcgcgaacg gtgcaaacgg atagccgtcc ggctgcgagc acccttccgg
     7801 cacaggatta ttcgatgtac aagaccaaaa tgggagatga aggccgcgaa atggttcaaa
     7861 tccccgaagg accctttacg atgggcagcc aggacggcga tcccgatgag acgccggagc
     7921 atcaggtgtt tctgaaaggg tttttcctcg atcgcaagga agtgacgcag gaagaataca
     7981 tgcggttcgc caagatgacc aagcgtggga ccccgaaaat cgaagtcttt gaagacgacc
     8041 agtccaagct gctgaaaccg gaatttgcgg cgatgagcgt ctcctgggat gacgcgctgg
     8101 cctattgccg gtgggcagga aagcgtcttc cgactgaagc cgagtgggaa aaggcgggac
     8161 gcggtgaggg gaaaagaagg tatccgtggg gggacaagtt cattacaaac ggtgccaacg
     8221 tggacggcag cgaagacgga tacaagtatt tggccccacc tggttctttc gatgcgggac
     8281 ggagccccta tgggttgtat gacatgacgg gcaacgtcgc cgaatgggtg aacgacactt
     8341 acgaagaaaa ctattacaag aaatcaccgt accgggatcc gaagggcccg gaaggcatag
     8401 gagatctgcg ggtggtgcgc ggcggctcgt ggcgcgagac ggaggctaat gcccggctgt
     8461 ccaagcgatt tgctgcgaaa cactggcgca cggatatcac catcgggatt cgttgcgcga
     8521 gcgatcttga ccagggaggc gctgctccca cttcataaag acggcatgct ggaaaccaaa
     8581 ttcaaggtcg ctcttctact gatcgtcctc gcctttgcag cgctccccgt gatgggcatt
     8641 cttcgcggaa cgacgacaac gccgtatgag gatgctgctc ctgccccgga cgaatcagac
     8701 ctgtcgcagc acgagtcatc atcccaggaa gtaccggtcc atgacgacat ggtgaccatt
     8761 ccggcaggac cgtttcttcg tgggacggcg agcggcggat ttgacgaaca gccacagcga
     8821 agcatcttcc tcgagacgtt ctccattgac cggtatgaag tcacgaatca ccactatcag
     8881 cagtttgccg ctgcaaccgg ccatcgcaaa gcggggccgc cgtctcgcta tgcgaagagc
     8941 atcggtcgga tgcgcgggac gaaccagccg atcatctacg tatcctggga ggacgccgct
     9001 gactattgcc gatggaaagg caagcggctt ccgactgaag cggaatggga aaaggcgatg
     9061 cgaggtccgg atggccggct atggccctgg ggagatcacg agaagccgaa cggagcgaac
     9121 tgggcccggg tgcaggacgg gtatgaagtg acggcgccgg tgggcacctt tcaaacggat
     9181 agaagcccct tcggagtgat ggatggcgcc ggcaacgtca tggaatgggt agccgattgg
     9241 tatcaggaag cctattacaa ggaatcaccg gacaagaacc ctcctggtcc tgaatatggg
     9301 acctacaggg tcatgagagg gggggcttac acgacggccg gggtagatct tcgcatcaca
     9361 agccgcagca agatggttcc ggattttcgg gatgaaacca tagggtttcg ctgcgcagct
     9421 tcagggattt tctcggggaa ttccggcgct cgtggaaaga gctaagtcta gagaaaatcg
     9481 aagtaataga ggatcagaaa cacggccaga atgatattga caaccatacc agccaaaact
     9541 ataatgtcga acacttttga gtttttattc atgcttttcc aacgtttcca cgctcaacga
     9601 caaattttga ataacacaca cactggtggg tgtcaagaaa gagcctgatg taatttcacg
     9661 tattttttcc ttatcttcaa gagagaagga gacacgatgg caacggcaac cgcaaccgct
     9721 ccagacagtg agatcaaaat caagatcggc aagatgattt tctacatcac ctgcgcgatt
     9781 ggtctgtggt tcttctattg gttcgccggc attcagtgcc cgtgctgaac agccagggcc
     9841 gccgaaacgc tctttagtca cttaccgttg gttactgagt tgaggaggag atgactatgg
     9901 gtcagacgat tgtttatatt ctgatttcga ccgtgatctg cgtcgcctac ttcacgctgg
     9961 tcgatcatta ccttatggat atgcaggggc tggattactg gtatctcttc cgcaagtaat
    10021 agcagcggag tcgggcctca cgcttgagtt tttggactgt tgcagatttt tatcggaatg
    10081 catcaaggag gtatcccatg ggtcctgtaa ccgccaagaa ggtgttgtca atcatggcgc
    10141 tctgcgcgat ggtgaccctt ctcgtgcttc ctgtggtcgt ctcgcttccc tcgccggcag
    10201 ccggtgagga cgcggcggct gtggatggag cgaagaagga tggggacaag gtcgagaagg
    10261 ggcgcgacgt gtattacaag acggaaggca tcgtggttgg tgcccccgcc ccgaagacca
    10321 cggatggtcc gaaggactat ccgaggtata acttcgagag ccgcgtgttg ctctggtttg
    10381 cgaaccagca gcacctctac tacggcagct tcgtgctggc cgtgccgatc ttctgtatgg
    10441 tgatcgagtt catgggggtc gtgagcaaag acaaggcgct cgcgaaacga tatgatcaat
    10501 tggcctatga cttcgtgaag atcagtctga cggcctactc tctgacggcg atcctgggcg
    10561 gcatcctcat cttcactttc ctcacgctct atccggcatt ttttgggtat ctctccagca
    10621 tcttccgacc ggtcatgcat atctatgcat tgatgttcgt agccgagagc ggcaccctct
    10681 acatctacta ctacggttgg gacaagatga aagaggggtt tttgaagtgg atccatctga
    10741 gcatgtcagt ggtgctgaac atcattggaa ctctcctcat gttcttggcg aattcctgga
    10801 ttggtttcat gatgtcgccg gccggtgtcg atgagcaggg aaggtttttg ggcaatatct
    10861 ggcacgtcat ccatactgcg ctatggaatc ctctcaacgt ccaccgcatt ctcggcaata
    10921 tggcgttcgg tggcggcgtc gtggccgcct atgccgccta taaattcctc gcatcaaaga
    10981 ccgatgagga tcgggctcat tacgattgga tgggttatat cgcaatggcg ctcggcgtgg
    11041 cgtttttgat tccgctgccc ttcgccggct actggctgat gcgcgaggtg tacgcctacc
    11101 gccaacagat gggaatcacg ctcatgggcg gtctgctcgc ctggttgttc atcatccagg
    11161 ccaccatgat cggcatcctc ttcttgagca ccaattatta tctgtggcag gcaatgggcc
    11221 gcatgcgcgg tgccgaaaag taccaacgct acatcaagta tctagtgttt ctcctggtct
    11281 gcgggttcct ggtgttcatt acgccccaca ccatggtcat gagtcctgcg gaactcaagg
    11341 cgatgggcgg ccagcaacat ccagtgctgg gcaattacgg cgtcatgtcc gccaagaacg
    11401 ggggcatcaa tgtcatcatc accacgaccg tgttgagctt cgtgtggtac atgcggggca
    11461 acaaggtttc cacggtgtcg tggtcgaagt ttggaaacat cttcatggga tgtttcttct
    11521 tcttcgccta tgtcaacatt atttggctgg cgatctacgg ctattacatc ccagccaacg
    11581 tccgcgtcgg actctccgtt ccacaagtgg caaccaccct gtcctgcctc ttcttcatgt
    11641 ttggactgaa tagcgtcatg atgaagggcg ccaagcaaat gggtccgatc gagtggggca
    11701 agatttccgc tcgttcacag tacgcgttga tcatgctggc gacggccttt acctggatga
    11761 tggggttgat gggctatatc cgctcctcgg taaggctgtt ctggcacgtc aacgagatca
    11821 tgcgcgataa ctctccctgg gcctacaccc atacggtggg atttgcggcg aacatgattt
    11881 cgttcaacgt gctgttcttc tggatcagca ttcttttcgt cttctggctc ggtagtttga
    11941 cggcgaagaa agtgccgatc ggggaaaaag tgggagttcc tggcggcgcg ccgcaacctg
    12001 ctggcagcca ctaaccatcc ctattgagtt tggctcgcgg tgggagacgc gatcacgtct
    12061 cccaccggtg tagccgcagg aggtttaaac cgtgggtaat ctgatcaatg aagctctctc
    12121 gatgggctgg gtggtcctcg ccctgctagc gggcttactc atctactttc aattctccat
    12181 cagcgatcct gccgccaaga agcgggcgac cttcaaaact ttcattggca tcatcgcggc
    12241 gatcttgatg ttcatcgcca tcgccaacta caagaacaac ttctatggag aaaaccggtt
    12301 gttgccggtc tctctcgtga tgattaccgt cacgaccttc gtcatggcca tatattttac
    12361 gaatctcgcc gcattgctga agatcggcgg gttcatgttc ttcgttgccg ccttcctctc
    12421 gggttacgga aactggttgc cgcaggttga aggtggattt cctcccaaag aagaaaagct
    12481 ggacttcagc tcaatgtcgc cccagcagct cgccgacgag ggtgaaaaaa tcattttcgg
    12541 tggcatcggg caaaacaagg tgcagggcgc aataggtaaa gggcaatgcc cgctctgcca
    12601 cgccttccat gccggtatgc tcggcgaacg ggcccccaat ctgctcggac taccggagcg
    12661 ggcgggaaag gaacggctcg aagatccgaa gtattcaaag ggtaacccgg ccaagcgtga
    12721 atacgccgaa aaagaatcgt gtccgggctg cggtaccgcc gaaaacggcc aggaatacat
    12781 cgccgaatcg cacgcgtgtc ccagttgcta tgtggtgtcc ggttacggtg taaagggtac
    12841 caacgataaa gaaagcccga tgccaaagat ccataagcca ccgatctccc tctcgttggc
    12901 cgaattggcg gcggtggaca cgtggttgta tgtccgcgaa ggacgggaag cgccgtcatt
    12961 cgaggagatt acgaagtcct acgaaaaatt cattcccgaa gccgaacgtc ccaagcagca
    13021 agaagacaag cctgcggggg cagccagcgc gttactggcc gacgggtcgg agccggttga
    13081 ccagattttt gccaaggcgc aatgcgtcgt ctgtcacacg attcccggca ttccgggagc
    13141 gaccggcacg cagggtccga aactggaaga ggccacgaac gcacctcagc ggatcaagga
    13201 tccggcgtac aagggaacgg cacatagcac tccagagtac atcatggaat cggtggtcgc
    13261 tcccagcacc tatgtggtga agggattccc cgacaacctg atgcccaaga ttttcggaca
    13321 aaagctgagc gccggagcgt tgaagaagat cgtcgactat ttgtcgcagg tgaaggcggg
    13381 cgcaccgcca ccgaagattt cgtaaatctt ggccgtctat ctgtagacat aagggagttt
    13441 acccatgaaa gcattgatgt cagtgggcgc cttggtggga gtcgtcggac ttctcctgct
    13501 tgtcgggatg atctttgatg tcgttccctc caataccgtg cgcttggtcg aaggctatat
    13561 gccaatgcag atgttatttg agttgacgct cttcgtcgcg ggttttgccg gtctcagtta
    13621 catgatgagt tcgatgggga tgccgctccc gaggttctgg cagggcatcg gattctgggc
    13681 gttcattctg ctgtacctga agtaccgggt atatcctccg atcccgttta gcgtgcgcgc
    13741 catgtatggc acagtctccc tcgtcgccgt cttcatgtgg gtgtcctcga acgaggagga
    13801 ctggaagaaa ttcaagcagc cgattttgaa cgtccttgat gcgcagagcg gcggcaataa
    13861 ggtgctgcgg tacgcctatc tcatcctgct tccaattctt atcggcggat tttcgtacaa
    13921 cgcgatgaag cccaagtccg aggagccgat tgagttgcgt accgtgcatc cagcgccacc
    13981 ggccagcacg aaggtgcatg gcaagaccta tgtgcttcag acgtcacaaa atccgtatcg
    14041 cgtcaatccc gagggaaagt acgatcagga atttacaaat gccaatatcg tggaacaggg
    14101 tatgggccgg ttgatgaagc ccaatgcgaa cccctgggat gaaaagagca cggggtatct
    14161 gaagtatgtc cgtgagggcg gggagatttt cttccagaac tgccatttct gccacggtga
    14221 taacttgaac ggtcgcgggt tgcacgcctt tgccttcaat cccatcccgg ccaatttcac
    14281 cgatccggga accatcgccc agttgcaaga gacattcatc ttctggcgcg tggccaaggg
    14341 cggcatcgga ttgccgaacg aaggctttcc atgggcgtct gtgatgccgc cttgggaaca
    14401 gcacctgaca gtcgatgaaa tctggaaggt gatcttgttc gaatattggc acacgggcta
    14461 ctacccccgt acctgggact aatcgtctaa gggattcata acctggatct atatatattg
    14521 agatgagggt aaacatgatg aacagttcaa gtcggaaggc aggagtggtc gcagtcgccg
    14581 ccttcggaat tatcctggcg tctggaagcg tcggcgttcc gctgatcgga gccgaagaca
    14641 tgcctgaagg gttcaagaag ggcgaactgg ccccggagcc ctctgccgag atgatcgagg
    14701 cgggcaagcg cgtctacttt acgaagtgtg tgtggtgtca cggcgtcgac ggtgcaggtg
    14761 atgggccggg cgccgatcgt ctctggcccc gcccgcgcaa cttcaatcaa ggcacattca
    14821 aaatccggca caccgcgagc ggagagcttc ctttgttcga ttcccggaag cccgttccgg
    14881 gacagaacga cttgttcgag accgtcacgc atggtttgcc gggctcagcc atgccgtcgt
    14941 gggagggtat tctgacggaa gagcaaaggt tgcaagtgct ctcattcgtg acaacccaat
    15001 tggtcaaa
//
LOCUS       DASSCQ010000109        10145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_484508, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000109 DASSCQ010000000
VERSION     DASSCQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484508"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..291
                     /locus_tag="VFH05_07825"
     CDS             31..291
                     /locus_tag="VFH05_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HET6678160.1"
                     /translation="MRKSPWLLLVFLLIGGLLGGILGEILRVMAPQGTIQTIFATNFT
                     PGISPPLTIDLVLLKITLGFSFKISLLSILGMLLGIYLYKNV"
     gene            complement(356..2362)
                     /gene="uvrB"
                     /locus_tag="VFH05_07830"
     CDS             complement(356..2362)
                     /gene="uvrB"
                     /locus_tag="VFH05_07830"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET6678161.1"
                     /translation="MSPFQLEAPFKPCGDQDQAIDQLVAGIQAGRKHQALLGVTGSGK
                     TFTMANVIARVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QTDTYIAKDSSINDAIDQMRHAATTSLLQRNDVLIVSSVSCIYGLGSPEVYHGMVVYL
                     EEGMEIRREKILAKLVEIQYARNDVDFHRGTFRARGDVIEIFPASNEAVSVRIELFGD
                     VIDAIHEIDPLTGKSIGKLPKVAIYPNTHYLIAPDRYQRATTGIEEELDERVAYFKTR
                     NQLLEAQRLTQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGEPPPTLIDYFPKDFLL
                     IVDESHATIPQVGGMYEGDYSRKRTLVDYGFRLPSAVDNRPLKFHEFERCMNQVIYVS
                     ATPGPYELEHTGGVIVEQIIRPTGLLDPVIEVRPAKGQVDHLLGDVRAEIALGGRVLV
                     TTLTKRMAEDLCEYYHELGIKVRYLHADIKTFERAEIIRDLRLGHFDVLIGINLLREG
                     LDLPEVSLVAILDADKEGYLRSHRSLIQTAGRAARNVRGRVLMYGDVITDSMQRAIDE
                     TSRRRAIQAAFNEAHGIVPASIQKEVRQLDYAEAAQEPEQLTLVAEAAVDYPTEEGPV
                     EQQIRRLEVEMKAAAKDLEFERAAVIRNRIRALKLRDLESKSGA"
     gene            complement(2473..3804)
                     /locus_tag="VFH05_07835"
     CDS             complement(2473..3804)
                     /locus_tag="VFH05_07835"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6678162.1"
                     /translation="MSTFAQMTLPPFLAQRLLQAGITSPTPIQQAAIPPALEGRDVMA
                     QAKTGSGKTLAFLLPMIERALRPSMASPAASVASVGAGKRGPQFLILAPTRELALQIE
                     TELRKYAPPAVTSLAVYGGTPIERHYRALRHFPKVVVGTPGRLLDLAGSGHLYLGAVT
                     WLVMDEADQMLDRGFLRDIQRILTLLSPGRQTLLFSATFSAEIQQLAGSMQRNPARIS
                     VDPGISSPTTISHAYYVVPAEQSRTQLVHTLLQALKGGERSMIFCDQKYKVRRLAAKL
                     GGETASVGAITGNHSQAQRERTLGAFRAGRLTSLVATDVAARGLDIPDVAQVIHYELP
                     VNPNSYVHRAGRTGRAEKEGATFLILSQAEEREYLRMVRQLRIQTTKLSLPALATLPV
                     PAAISEERPARRPTQRRHSHHAPEALHEARNGFGRGQRPAFRSEKPRRFGR"
     gene            complement(3950..4249)
                     /locus_tag="VFH05_07840"
     CDS             complement(3950..4249)
                     /locus_tag="VFH05_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6678163.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAAHGSVASARIITDKFTGQSR
                     GFGFVEMSGDSEAQAAIAALNGTQLGGRTLTVNEARPQEPRSGGGGRGGFGGGRH"
     gene            complement(4493..5281)
                     /locus_tag="VFH05_07845"
     CDS             complement(4493..5281)
                     /locus_tag="VFH05_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678164.1"
                     /translation="MSRRLPMSQNVVYLLLAVGWMLVQDGVVPVRVSAIPMENQAEKQ
                     ANFKQQALGGLFHRWTFDQQKPGDTLSGFSQFIVGSNASPAWTVSAEAEAPSPPNILK
                     AESSCQTGICYQLLVADQLHYEYPDVTVRLRFPAEGGIGQGGIVIGLQDERHFYAAVV
                     DLAAKKVEVVRVLDDQVAVLGETAITPKAVEWHSLRMNRDTIISKDVIEVFFDGRLLL
                     SVQDQTLGLGQVGLLVRGTSPMYFDSLHAVPLFSQRPVSSPAAY"
     gene            complement(5278..5541)
                     /locus_tag="VFH05_07850"
     CDS             complement(5278..5541)
                     /locus_tag="VFH05_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678165.1"
                     /translation="MAKGEHSTGIYDHLYRRFFANRDRHPGYDFQRAPGGSAQPPAVT
                     FPEKLRWWSLDRWRRRKKILSERDRLQREIVEMSAGRSKERRP"
     gene            complement(5547..7592)
                     /locus_tag="VFH05_07855"
     CDS             complement(5547..7592)
                     /locus_tag="VFH05_07855"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6678166.1"
                     /translation="MIERASNRLASESSPYLRQHANNPVNWYAWGAEALSRAKAEDKP
                     ILLSIGYSACHWCHVMAHESFENAEIAALMNQHFINIKVDREERPDLDDIYQKSAQVF
                     TGRGGGWPLTMFLTPAQEPFYGGTYFPPVPRHSLPAFPDVLRGVLEAYRSHKDEVQKN
                     VERVKGGLLKVGVPTGCQEVTERLLDQAVSELGQLFDPLHGGFGEGPKFPTVPPLSLM
                     CLRSVRTKDSRLLDMVLLQLRRMADGGLYDHLGGGFHRYSVDAEWKIPHFEKMLYDNA
                     QLVRLYLDGWRLTKEEGFRRVVEETLDYVRRELMHSDGAFFAAQDADSEGREGAYFLW
                     THDEIVSELGPDLGGEFCRAYGVTTEGNFEGKNVLHRLEGGRLTTEEQEAAESTLKPA
                     REKLLAARERRVKPLRDSNILTSWNAMMISAMFDAAVTLNQPTYRLAAEKALTFLLDY
                     AFVHGRVYHTVVEGKGRLNGYLDDVAWLAAALLDAFEATSHRWYLDQARLVTDSIESH
                     FWDEAGGGGCFFTSHDHERLIQRMKTSTDSAIPAGNAVTASVFLRLFSLTGEERYHER
                     AGKILRLFERGMSQNPYGSAALLCSVDWWLSGPREIVIVGARGHAMTEAMLRTVSQRF
                     IPHRVVLVREEANAGDAHEFPLGKGKVSLNGRPTAYVCQRQTCSQPVVDLQQLEGML"
     gene            7707..9254
                     /locus_tag="VFH05_07860"
     CDS             7707..9254
                     /locus_tag="VFH05_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6678167.1"
                     /translation="MKLIHLFHVLCLHCICLTSAFAGSPLDSSISSSDWLPTALNQIS
                     VERMLADISVLSGPTLAGRQTGSLEDERSAAFVTHRFSELRLHRAPATPRQSLTNPLP
                     QQEWKQTHSVPTRKIGEDVLLELVRPQERRSLVTGTQFLPVLDSPSADVRGPIVFVGY
                     GIADPSRGVDDYAGVDVRNKIVLFLRGKPDRYAAAVAQTDKERVAKEKGAIAYLVATG
                     PILTAYEQRRGVTGAPSAFYSTTPSTTQLPGAWISTETAEWILEQGATGTTTLHTVQE
                     ELNRTGLSKPVVTEVTAQMRWTSSLEPGTLYNVMSIIRGHTVHSDETVLVGAHRDHFG
                     RQAGLLFSGADDNASGTAVLLEVARVLSLAPAAPQRSVLFVSFSGEEQGLLGSRLYVT
                     QPIVPLLNTIAMINVDHAGTGNGRLTVGVSGLEKSTAERIGQLTGLSDKLDVFGFFPG
                     GDHVPFKEAGVPTVTVVSGGAHPHFHQAGDTVDTINADILQAVARYVLALTWQLANDT
                     PHPRVPH"
     gene            complement(9429..9794)
                     /locus_tag="VFH05_07865"
     CDS             complement(9429..9794)
                     /locus_tag="VFH05_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678168.1"
                     /translation="MVLWFCGPEQPASAQTISSVRSFDGGVAPLFSLTGPGSLYLDNQ
                     GTQGFLYNPGNNFQSYSFRNPTTGQAWSGAVMTLGPQLSIGLIQGANQVGSPVVLPGP
                     PRQPPPLVDVQSTLLDDIP"
     gene            9902..>10145
                     /locus_tag="VFH05_07870"
     CDS             9902..>10145
                     /locus_tag="VFH05_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678169.1"
                     /translation="MRSRLAAGLPGVLLLSAASTLAMTARPALAAEDVTKATPTERRE
                     EISLSEAALRALKNNLDISISRHTKESRLTDIVVEQS"
BASE COUNT         2158 a         3011 c         2857 g         2119 t
ORIGIN      
        1 atgcagtcaa tagtttaaga ggccgtcgac gtgagaaaat cgccctggct gctgctggtc
       61 ttcctcctca tcggcggctt gctcggcggt atccttggag agatcctgcg ggtgatggcc
      121 ccacagggga caattcagac cattttcgcc acaaacttca ccccaggaat cagtcctccc
      181 ttgacgattg atctggttct cctcaagatc acactggggt ttagtttcaa gatcagtctc
      241 ctcagcattc tgggcatgct cctcggcatt tatctgtaca agaacgtcta acggcaggca
      301 aacggtgcga gacgttggac ctcgacgaac cgcctgtgcg gacaaggggt aggcgctagg
      361 ccccggattt tgactccagg tcccgcaact tgagtgctcg gatgcggttg cgtatgacag
      421 ccgcgcgttc gaactccaga tcttttgcgg ctgctttcat ctctacttcg agccgccgga
      481 tctgttgttc gactggcccc tcctctgtcg gatagtcaac ggctgcttcg gccaccaacg
      541 tcaactgttc aggctcctga gcagcctccg catagtccaa ctgccgcact tctttctgaa
      601 tactcgccgg gacaataccg tgtgcctcat tgaaagcggc ctggatcgcc cggcggcggg
      661 aggtttcgtc gatcgcccgc tgcatcgaat cggtgatcac gtcgccatac atgagcaccc
      721 ggccgcgcac gtttcgagcg gcacgtcctg ccgtttggat caaggaacga tgcgaccgca
      781 ggtagccttc tttatcggca tccagaatgg ccaccaggct gacttccggc aggtccagtc
      841 cttcccgtag caggttaatc ccgatgagga cgtcaaaatg gcccaaccgg agatcacgaa
      901 tgatctcggc tcgttcgaac gtcttgatgt ccgcatggag gtagcgcact ttgatgccaa
      961 gctcgtgata gtactcgcac agatcttcgg ccatgcgctt ggtcagcgtc gtcaccagca
     1021 cgcggcctcc tagggcgatt tcggcccgga cgtcgcccag cagatgatcg acctggcctt
     1081 tcgcgggtcg cacctcgatg acaggatcga gaaggcccgt cggccgaatg atctgctcaa
     1141 cgatgacccc gccggtgtgt tccaactcat agggacccgg tgtggcggac acatagatga
     1201 cctggttcat gcaacgctcg aactcatgga acttgagtgg ccggttgtcg actgcagaag
     1261 gtagacgaaa cccatagtcc accagtgtgc gtttacgcga gtagtcgcct tcgtacatcc
     1321 cgccgacttg cggaatggtc gcgtgcgatt cgtcgacgat aagaagaaag tccttgggaa
     1381 agtagtcgat gagggtcggc ggtggctctc ccggagcgcg gccactcagg tgccgggaat
     1441 agttctcaat cccatggcaa tagcccatcg cacggatcat ttctaaatcg aatttcgtcc
     1501 gctgcgtcag gcgctgggct tccaacaatt ggttgcgtgt cttgaagtag gccactcgtt
     1561 catcgagctc ctcttcgatg cccgtcgtcg cccgttgata gcggtccggc gcgatcagat
     1621 agtgggtgtt cggatagatg gccactttcg gcaattttcc gatcgacttg cctgtcagcg
     1681 gatcgatttc atgaatggcg tcaatgacgt cgccgaatag ttcgatgcgg acggacaccg
     1741 cttcgttcga ggccggaaag atttcaatga cgtcgccacg ggctcggaag gtaccgcgat
     1801 gaaaatcgac gtcgttgcgt gcatattgaa tttcgacgag ttttgccaga atcttctcac
     1861 gcctgatctc catcccttct tcgagataga cgaccatccc gtgatacacc tccggagaac
     1921 cgagaccata aatgcacgac accgatgaga cgatcaacac gtcgttgcgt tgcagcagcg
     1981 acgtcgtcgc cgcatgccgc atctggtcga tcgcatcgtt gatggacgaa tccttggcaa
     2041 tgtacgtgtc cgtctgcggg atgtaggctt ccggctggta atagtcgtaa tagctgatga
     2101 aatactcgac tgcattgtgg gggaaaaatt gcttgaactc ttggtacagc tggccggcga
     2161 gcgtcttgtt atgtaccagc acaagcgtcg gtttttgcac gcgtgcgatg acattggcca
     2221 tcgtgaacgt tttgccggac ccggtcacgc caagcagagc ctggtgtttc cggccagcct
     2281 ggatccctgc cacgagctga tcaatggcct gatcctgatc cccgcagggt ttaaatggag
     2341 cttcgagttg gaacggtgac attgaggcgt cctacagtgg ttactgtacc atgcggactg
     2401 ggagcccgtc ctgagaagcg tatcacgacc aaccgctcag tcgatcgtga tgcggcgatg
     2461 tgcgggcgac gattatcgcc cgaaccgtcg gggcttctct gaacgaaatg caggtcgctg
     2521 gcctcttccg aagccgtttc gagcttcatg aagcgcctct ggggcgtgat ggctgtgacg
     2581 gcgctgggta ggacgccgtg caggccgctc ctcgctgatc gcagccggta caggcagtgt
     2641 ggccaaagcg ggaagcgaca gcttcgtcgt ctgaatcctg agttgacgga ccatacgaag
     2701 atattcccgt tcctcagcct gagacagaat gagaaaggtg gcgccttcct tttccgcacg
     2761 acccgtccga ccggcacgat gcacgtacga atttggattg accggcagtt cgtagtgaat
     2821 gacctgcgcc acatccggga tatcgagtcc ccgggccgcc acatctgtgg ccaccagaga
     2881 tgtcagccgt cccgctcgaa aggcaccgag cgtgcgttcg cgctgggctt ggctgtgatt
     2941 gccggtgatc gcgcccaccg atgcagtctc cccgccaagc ttggcggcca accgccgaac
     3001 tttgtacttc tgatcacaaa aaatcatcga tcgctctccg cccttcagcg cttgtagcaa
     3061 ggtatgcacg agttgtgtgc gcgattgctc ggccggcacc acatagtagg catggctaat
     3121 ggtggtcgga ctgctaatgc cgggatcgac tgaaatcctg gcgggattcc gctgcatcga
     3181 gccggcaagt tgctggatct ctgctgaaaa tgtggcagag aacaacaacg tctgccggcc
     3241 gggcgacaat aaagtcagga tgcgttgaat gtcacggaga aagccgcgat ccaacatctg
     3301 atccgcctcg tccatgacga gccacgtgac cgcgccaagg taaaggtgac cggatccagc
     3361 caggtcgagc agcctgcccg gcgtgccgac cacaacctta ggaaagtgcc gaagcgcgcg
     3421 gtaatgccgc tcgattggag tgccgccata cacggcgagg gaggtgaccg ccggcggcgc
     3481 atattttcgt aattccgtct caatctgcag tgccaactct ctcgtcggag caagaatcaa
     3541 gaactgcggt ccgcgttttc cggcaccgac tgaggcgaca gacgccgctg ggcttgccat
     3601 gctcggccgc aaggcacgct cgatcatcgg tagaagaaag gccagtgttt ttccgctccc
     3661 tgttttggcc tgcgccatga catcgcgtcc ttcaagcgca ggcggaatcg ctgcctgctg
     3721 gatcggcgtg ggagaggtga tcccggcttg cagtaatcgc tgggcaagaa acgggggtaa
     3781 agtcatctga gcaaacgtcg acatagaact ccttatgtac atgagcttcc tgggactgct
     3841 aggcggccga tcattcggcc gagagacaca tctgaaagat gggcaattct aggaaggata
     3901 taagcggggc tgccggtgag gcagccccga catgaaaaac ggcttgcact cagtgccggc
     3961 cgccaccgaa cccgccgcgg cctccgccgc cagagcgtgg ttcttgcgga cgcgcttcgt
     4021 tgactgtcaa cgtgcggccg ccaagttggg taccgttcaa cgcggcgatc gccgcttgcg
     4081 cttccgaatc cccggacatt tcgacgaagc cgaagcctcg cgactggccg gtgaacttat
     4141 cggtgatgat tcgggctgac gccaccgacc catgcgcagc gaacagatcg ctcagttgct
     4201 gctcggttgt ggaatacggc aaacccccaa cataaatctt cgaacccatt gggttcctcc
     4261 tttgagaaaa tggtgttgtc ttggactcga gaagaggtga ggaaggaatg ggccgaagac
     4321 gcaaacacga tggcgactca ggtctggctt ccgatcagat ccccgggcag aaccaaaaca
     4381 acatcagtat caagccttgt cactgttcta ctgcatcacc accaggcctt tcgcggtgat
     4441 acaccgatgc actgtaacgt aggagcgatt caaatgcaag cccagactgg tttcaatacg
     4501 cggcagggct cgacaccgga cgctgagaaa acagcggcac ggcatgcaag ctatcgaaat
     4561 acatgggaga agttccacgc acgagcagtc cgacttgccc aaggcccagc gtctggtcct
     4621 gcacggaaag caagagccgt ccatcgaaga acacctcgat cacgtctttg ctgataatgg
     4681 tgtctcgatt catgcgtagt gaatgccact ctaccgcctt cggggtgatg gccgtctccc
     4741 cgagcactgc gacctggtca tcaaggacgc ggacgacttc caccttcttc gctgccaggt
     4801 cgacgacggc tgcataaaaa tgtcgctcgt cctgcaagcc gatgacaatt ccaccttgcc
     4861 cgatgccacc ttctgctgga aaacgcaacc gaaccgtcac atccggatac tcgtaatgca
     4921 attgatcggc caccaatagt tggtagcata tgccagtctg acaggacgac tctgctttga
     4981 ggatattggg tggagagggc gcctccgctt cggcggatac ggtccaggcc ggcgaggcgt
     5041 tgctgccgac gatgaactga gaaaacccac tcagggtatc accgggcttc tgctgatcga
     5101 acgtccagcg gtgaaacaat ccgccgagcg cctgttgttt gaaattggct tgtttctcgg
     5161 cctgattctc cattggaatg gcggagaccc tcaccggcac cacaccgtcc tgcacgagca
     5221 tccatccaac cgccaggaga aggtagacta cattctgaga catgggcaaa cggcggctca
     5281 tgggcggcgc tcctttgacc ggcccgcact catctcgaca atctcacgtt gcagccgatc
     5341 acgctcgctc aaaatcttct tgcggcgccg ccagcgatcg agcgaccacc agcgcaattt
     5401 ctccggaaat gtcacggccg gtggctgagc gctaccaccc ggcgcccgct gaaagtcata
     5461 gccgggatgt cgatctctgt tggcgaagaa tcgccggtag agatgatcat aaatacccgt
     5521 cgaatgctcg cctttcgcca tcgcgttcag agcatcccct cgagctgctg tagatcgacg
     5581 accggttgtg agcaggtctg ccgctgacag acatacgcag ttggtcgccc gttgagactg
     5641 accttgccct tgccaagcgg aaattcgtga gcatcgcccg cattggcttc ctccctcacc
     5701 agcacaacac gatgaggaat gaagcgttgt gacacggtcc tcagcatcgc ctctgtcatt
     5761 gcatgtccac gcgcgcctac gatcacaatc tccctgggtc cggacaacca ccaatccacc
     5821 gaacagagaa gcgcggccga cccataggga ttctgcgaca taccgcgctc aaagagtcgt
     5881 aggatcttcc cggcccgctc atgataccgc tcctcacctg tcaaagagaa gagcctgaga
     5941 aacaccgagg ccgtgacggc attcccggcg ggaatggcgc tgtccgtgct ggttttcatt
     6001 cgctggatca accgctcgtg atcgtgactg gtaaagaaac agcctccgcc gcctgcctcg
     6061 tcccagaaat gggattctat tgagtccgtc acgagccggg cctggtctag ataccagcga
     6121 tgagaagtcg cttcgaacgc atcgagcagt gcggccgcta gccaagcgac gtcatcgaga
     6181 tacccgttca gtcgtccctt cccttcgaca accgtatggt agacgcgccc gtgcacgaac
     6241 gcatagtcca ggagaaacgt cagcgccttt tcggcggcca agcgataggt cggctggttc
     6301 aacgtcaccg cagcgtcgaa catggcagag atcatcatgg cattccaact ggtcagaata
     6361 ttgctgtcac gcagtggttt gacccgtcgc tctcgcgccg ccagcaattt ctccctggcc
     6421 ggtttcaatg tcgattcagc tgcctcctgt tcttcggtgg tgaggcgtcc cccttcaagc
     6481 cggtgtagca cgttcttccc ttcaaaattc ccctcagtcg tgacgccgta ggcgcgacaa
     6541 aactcgccgc ccaagtccgg tcccagctcg gatacgattt catcatgggt ccacaaaaag
     6601 tatgcaccct ctcggccttc actatcggca tcctgtgcgg caaagaaagc gccgtccgaa
     6661 tgcatcagtt cacgtcgtac atagtccaag gtctcttcca cgacccggcg gaatccttct
     6721 tccttcgtca gccgccatcc gtcgagatat agacgaacaa gctgggcgtt gtcgtacagc
     6781 attttttcga aatgtggaat tttccattct gcatcgacgg agtaccggtg gaaccctccg
     6841 ccaagatgat catacagacc tccatccgcc attctccgca gctgaaggag aaccatatcg
     6901 agcagtcttg agtctttggt cctgacggaa cgcagacaca tgagactcaa cggtgggaca
     6961 gttggaaatt tcggcccttc accgaacccg ccatggagag gatcgaacaa ctgacccagt
     7021 tcgctgaccg cctggtccaa aagtcgctcg gtcacctcct gacaccccgt gggaaccccg
     7081 actttgagca agccgccctt cacccgctca acgttttttt gcacttcatc tttatgacta
     7141 cggtaggctt cgagcactcc acgcaagacg tcaggaaacg ccggaagaga gtgacgcggc
     7201 accgggggaa agtaggtgcc tccatagaag ggttcttgcg cgggcgtcag aaacatggtg
     7261 agcggccaac cgccgccccg accggtaaag acctgggcag acttctggta gatgtcgtcg
     7321 agatcaggac gctcctctcg atccactttg atgttgatga aatgctgatt catcagtgcg
     7381 gcgatctcag cgttctcgaa cgactcgtgg gccatgacgt ggcaccaatg acacgcggaa
     7441 taaccgatcg agagcaagat aggtttgtct tccgccttgg cacgggagag cgcttcggca
     7501 ccccaggcat accagttcac ggggttattc gcgtgctgtc gaagataggg acttgactcg
     7561 gaagcgaggc gattggacgc tcgttcaatc atgatggatc gaaaatgaat cgctccgttc
     7621 ggcgctggcc atactatcct gccgactcct cacctgcaat gaatctcaca gtccatgcta
     7681 tagtgccgac tcgagacgcg gacgccatga agctcattca cctattccac gttctctgtt
     7741 tgcactgcat ctgtctgacc tcggcctttg cgggttcccc gctggactcg tcgatctcgt
     7801 catccgattg gcttcctacc gcattaaatc agatctccgt tgagcgcatg ttggcggata
     7861 tcagcgtatt gagcggaccg acccttgccg gtcgtcaaac gggcagcctg gaagacgaac
     7921 gttcagccgc cttcgtgact catcggttct ccgaactccg cctgcatcga gccccagcga
     7981 ctccccgaca aagcctgacc aacccgctcc cacaacaaga atggaaacaa acccactcgg
     8041 tgcccacacg aaaaatcggc gaagacgtgc tgcttgaact cgtgcgtccg caggaacgac
     8101 gctcgcttgt aacaggcacg caatttttgc ctgtcctcga ctctccctcg gccgatgtcc
     8161 gtggcccaat cgtgttcgtc ggatacggaa tcgccgatcc atctcgcggc gtcgacgatt
     8221 atgccggcgt cgacgtacgc aacaaaatcg tcctgtttct tcggggcaaa cccgatcggt
     8281 acgcggcagc cgtggcgcag acggacaagg agcgggtggc aaaggagaag ggggcgatcg
     8341 cctatctcgt ggccaccggg cccatcttaa cggcctatga acaacgccgg ggcgtgaccg
     8401 gtgcacccag tgccttctac tccacgaccc caagcaccac acagttgccg ggcgcgtgga
     8461 tcagcaccga gacagcggaa tggattctcg agcagggtgc aacaggaacg acgacgcttc
     8521 acaccgtcca ggaagaactc aaccggaccg gtctctccaa gcctgtggtg acagaggtca
     8581 cagcccagat gagatggacg agcagtcttg agcccgggac tctctataat gtcatgtcca
     8641 tcattcgagg ccatacggtg catagtgatg aaacggtgct ggtaggggca catcgcgacc
     8701 acttcggtcg gcaagcgggt ttgttgttct ccggggcgga cgacaacgca tccggcacgg
     8761 ccgtgctgct cgaagtcgcg cgagtccttt ctcttgctcc ggcggcaccc cagcggtcgg
     8821 ttctctttgt gtcgttcagc ggggaagaac agggcctcct aggctcgcga ctgtatgtga
     8881 cacaaccgat cgtccccttg ctgaacacga tcgccatgat caacgtggat catgccggga
     8941 ccggcaatgg gcggttaacg gtgggagtct caggtcttga gaaaagcacg gcggaacgga
     9001 tcggccaact gaccggtctt tcggacaagt tggatgtgtt cggctttttc ccgggcggcg
     9061 atcatgttcc gtttaaagaa gctggtgtcc cgaccgtcac cgtggtcagc gggggcgcgc
     9121 acccgcattt tcaccaagcc ggcgatacgg tggacaccat caatgcggac attctccaag
     9181 cggtagcgcg ctatgtgctc gccctcacct ggcagctcgc gaacgataca ccacatcctc
     9241 gcgtgcctca ttgaaatcga tcagcgaacg ctgaaggctg atgcgagccg ttcgttctag
     9301 ggtttcgtga gctcacttat cttcattgaa accgctcgcc gcacttcttt tcggaggacc
     9361 agcttgctca ttgtgttgat ccacccgagg tgggatggac ggaatacatt ttgggtccgg
     9421 atgctcgact agggaatgtc gtcgaggagg gtcgactgca cgtcgactag cggcggtggt
     9481 tgtcggggag gaccgggaag gacaacgggt gaaccgactt gattggcgcc ttggatcagt
     9541 ccgatcgaca attgcgggcc cagggtcatg accgcgccgc tccatgcttg accggtcgtg
     9601 ggattacgga aactgtatga ctgaaagttg ttaccgggat tatagagaaa tccctgcgtg
     9661 ccctgattgt ccagatagag acttcctgga ccggtgaggc tgaagagcgg cgcgacgccg
     9721 ccgtcgaatg aacgtacact cgaaatagtt tgcgccgaag cgggctgctc tggaccgcag
     9781 aaccaaagca ccatcgagac agcaagcact aaccccaagc gccttgcaag aagttcatga
     9841 cagatgtcca tgatgcgatt atagtatggg accacgaggc ttgtcgacaa aaggaggttc
     9901 tatgagatct cgactcgctg ccggcctccc tggtgtcctg ctgctctctg ccgcgagcac
     9961 gctggcaatg accgcccgac ccgctcttgc tgccgaggac gtcacgaagg ctacacccac
    10021 cgagcggcgg gaagagattt ccctttccga agccgccctc cgggcactca aaaacaatct
    10081 ggacatcagt atcagccgcc acacgaagga gagccgcctg accgacatcg ttgtcgaaca
    10141 gtcga
//
LOCUS       DASSCQ010000110         8956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_676186, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000110 DASSCQ010000000
VERSION     DASSCQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676186"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFH05_07875"
     CDS             <1..894
                     /locus_tag="VFH05_07875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET6678170.1"
                     /translation="EKAEIARRYLWPRRLKEAGLEADHVQLPDGVLDRIISRYTREAG
                     VRQLEQMLGRLTRKIALTVAELPESHVRETVVVEEDKLPDWLGSERFMPEEARKHIPV
                     GVATGLAWTPTGGDVLYIETTLLPGSHEVSLTGQLGDVMQESARAARSYLWSHAESMG
                     LDISRFKRNGLHIHVPSGAIPKDGPSAGITMATALASSYVGKPVRSDTAMTGEISLSG
                     LVLPVGGIKEKVLAAHRAGIKRIILPKANEKDLKEVPQEVRDDLTFIFVERIEEVLPS
                     AFDQDLGNQARPTEHHASTAL"
     gene            1003..1428
                     /gene="msrB"
                     /locus_tag="VFH05_07880"
     CDS             1003..1428
                     /gene="msrB"
                     /locus_tag="VFH05_07880"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET6678171.1"
                     /translation="MPQKIDIGPIVKIVKSDDEWKKLLATEAYQVLRHEATERPFTNN
                     MHDNKQAGIYHCAGCDLPAYSSEHKFDSGTGWPSFWQPIDPKVVETHTDFKLIFPRTE
                     VHCARCEGHQGHIFKDGPKPTGLRYCINGAALKFVPASA"
     gene            1570..2454
                     /locus_tag="VFH05_07885"
     CDS             1570..2454
                     /locus_tag="VFH05_07885"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6678172.1"
                     /translation="MAHVGFVGVGRMGANMARRLQDKGYRITAVHDRNQQAADELSRE
                     LGCEAVETPARVAELSEVVVTVVSDDAAMHDIYREGASNSLLAHAKGRLFINCATVSP
                     DVHIATEQVVERHEGASLEACMASSITQARQGTLYLMCAGKESVFERARPMLEVMSAH
                     LRYIGRTGEAAKVKALVNMVMNCNTAALAEGLGLGAALGVDLTMLRDVFSQTGAASRV
                     LETDGQDMQARAHDCYFSAAHATKDSGIALGLAATVGLTLPLAEATHRQYQRLVAAGG
                     GDLDKSAIAELTFKGRHQ"
     gene            complement(2484..3263)
                     /locus_tag="VFH05_07890"
     CDS             complement(2484..3263)
                     /locus_tag="VFH05_07890"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6678173.1"
                     /translation="MLLKRGILSLIVLLAAQPGWAGIDETKLIDLTYALDEQTVFWPT
                     NRPFTWEKAAWGRTANGYWYASGDFSMSEHGGTHIDAPIHFAEGRLAVDEIPVQKLIA
                     PAVVIDVRSAVEENADYRLSMRDLETWEARHGPIVQGAVVLMLTGWGKRWPDPVRYLG
                     SSTPSDPKTLHFPGFSKEAADFLVKERHVDGIGIDTPSIDYGSSRDFIVHQIINGANL
                     YGLENVANLEKVPPKGAILVALPIKIRGGTGGPVRIIAILP"
     gene            3329..5500
                     /locus_tag="VFH05_07895"
     CDS             3329..5500
                     /locus_tag="VFH05_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6678174.1"
                     /translation="MPNVLLLIPPLTQLNTPYPSTAYLTGFLRSQGVLCEQADLGIEM
                     VLRVFSREGLRQVFYQLKPRRRSLHPVAQEMIANQTAILDHIDAVVAYLQGKAPELTK
                     SFARRAFLPEGPRFRMQRSWSRSTSTEDRAKHAATLFLEDLTDLVQGTITPHFALNRY
                     AEHIARSAASFDSMLAALQAAPALTDQFMLEALWAHVERVNPLLVGLSVPFPGNLYGA
                     FRIAQSLKQNRPDILIALGGGYVNTELRRLSDVRVFDFVDYVTLDDGERPLLCLLEHL
                     SGKRPVQALSRTYYRDNSRVVFADDVTLGNIAMNDAGCPTYRGLMLDRYLSILDSTNP
                     MHRLWSEGHWNKLTVAHGCYWKQCTFCDVGLDYINRYEITPTERLIHNIQQLADETKR
                     LAFHFVDEAAPPMALKALAIALLQRKIGISWWGNIRFEEAFTPDLCRLLAASGCIAVT
                     AGLEAASDRLLAKMKKGITVDQTARVAAAFRQAGILVHAYLMYGFPSETVQETIDSLE
                     RVRQLFAADLVQSAFWHRFTVTAHSPIGLDPRGNGLRIVGPEFGGFAENDLLHEDATG
                     PTPEWIGAGLRRSMLNFLESRGLAMEVTKWFDHPTPRPRVSPTWISRALGKRIPDEEF
                     AAERRCVWIGEQACVESGTGHIKLSIQGRRRALTATLPSRQASWLSELLNEALPRRDR
                     YASYPSWRDAKLAFPGRATDFASFVRDPSWRRIRSAGLLLV"
     gene            complement(5531..6544)
                     /locus_tag="VFH05_07900"
     CDS             complement(5531..6544)
                     /locus_tag="VFH05_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET6678175.1"
                     /translation="MRAVGLYRHLAITDPESLLDLDIPLPEATGHDLLVRIEALSVNP
                     VDTKVRAPGKPDRQTPLVLGWDAAGVVESVGQAVTLFRPGDSVYYAGDISRPGSNSQY
                     QLVDERIVGRRPRSLAAEAAAALPLVAITAYEGIQERLGIDFEGGSKGRTLLIIGAGG
                     GVGSMAIQLGKIAGLSVLATASRPESIAWVKQLGADEVLDHRRPLRPQLEGIGFGDVN
                     FILNCADTDGYWDSMAELIAPQGKICTIVENKGLLQQQALKAKSITHVWEFMFTRPKY
                     QTADMIEQHRLLNRVADWIDRGTLKGILRETLHPINAENLKKAHAKLESGTMIGKLVL
                     KDW"
     gene            complement(6622..7257)
                     /locus_tag="VFH05_07905"
     CDS             complement(6622..7257)
                     /locus_tag="VFH05_07905"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET6678176.1"
                     /translation="MALRLGDEAPNFTAETTEGTINFHEWLGNSWGILFSHPKDYTPV
                     CTTELGTVAKITPDFKKRGVKVIAVSVDPLDSHKGWINDINETQHTTMNYPIIADPDK
                     KVAALYDMIHPNALDNMTVRSVFVIGPDKKIKLTLTYPASCGRNFDELLRVVDSLQLT
                     SKYKVATPANWKEGEDCIITPAVSNDEAKTLFPKGFKTVKPYLRYTPQPNK"
     gene            complement(7348..>8956)
                     /locus_tag="VFH05_07910"
     CDS             complement(7348..>8956)
                     /locus_tag="VFH05_07910"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET6678177.1"
                     /translation="VSLKDIPIQPFQPYVEQYARIAVDTGALDLDGTLKFAAEHPNGP
                     LMAFKGNLGVKALAIADRDDGAPVASWKQVQLREIAVALEPTSVTIEEVGINQPTVHL
                     AIDRDGVPNIKKLLSGSKAKNEPMGTERSASPTKKAPPPSVTIKTVKVLRGTATFQDE
                     SIAPSVQAGLYDLTGTVKGLSSKQMARAEVDLSGKIDKVAPLKIAGTINPLTEDTFTD
                     LTIKFDNVDLTTAAPYTGKYVGYPIRKGKLFLDLVYKVSQKQLEAENKVAVDQLTFGE
                     KTDSPDATSLPVPLAIALLKDRQGRIDIDLPIRGDLKDPDFRYGKAVWATLGNLLSKM
                     VASPFALMGKLVPGGGDGEELQHLTFEPGSAAIKPSEMKKIDALAKGLEERPGLRLEI
                     TGTADPTRDRQAVALQRLQDLLRSRWRQENGNTQESDLPLSAEARLITKLFDQRQSQQ
                     PMEPRPPDAPVQPPTLEEMKRNLAESIRVDDDTLRSLARARAEQVQAVIVGDGKLPEE
                     RVFLTDVDLTTSDDGKVQSRLNITAGS"
BASE COUNT         1849 a         2598 c         2521 g         1988 t
ORIGIN      
        1 gagaaagccg agattgcgcg gcggtatttg tggccgcgac gactcaaaga agcgggtctg
       61 gaggcggatc atgtccagct tcccgacgga gtgttggacc gcatcattag ccgctacaca
      121 agggaggctg gtgtccggca attggagcag atgctgggcc gattgacccg caagatcgcc
      181 ctcacggtag cggaacttcc cgaaagccat gtgcgggaga ccgtcgttgt ggaagaggac
      241 aaacttcccg actggctggg atcggaacgg ttcatgccgg aggaagcgag aaaacatatt
      301 cccgtgggcg tcgcgaccgg cctcgcttgg acaccgaccg gtggcgatgt gctctatatc
      361 gaaaccaccc tcctgcccgg aagtcatgag gtcagtctga ccggtcagtt gggtgatgtg
      421 atgcaagagt cggcgagggc cgcacgaagc tacctgtggt cgcatgccga aagcatggga
      481 ctcgatatct cacgattcaa aaggaacggg ttgcatatcc atgtcccttc aggagccatt
      541 ccgaaggatg ggccttcagc gggcattacg atggcaaccg cactggcgtc gtcttatgtg
      601 gggaagcccg tgcgcagcga taccgccatg accggtgaaa tcagcctcag cggcttagtc
      661 ctgcccgttg gcgggatcaa ggagaaggtg ctggccgctc atcgggcagg cattaaacgg
      721 atcattcttc ccaaggcgaa tgaaaaggat ctcaaagaag tgcctcaaga agtacgggac
      781 gacctgacgt ttatttttgt cgagcgaatc gaggaagtac tcccctcggc gttcgatcaa
      841 gatctgggaa accaggctcg gccgaccgag caccatgcat cgactgcttt gtaatgggaa
      901 agggagcaag gcggccgggc cggccgcctt gctcctcgat caatacgtat gccacgatgc
      961 cacgctctcg catctagcac ctgagttacc cgggaggttg tcatgccgca aaagatcgac
     1021 atcggcccta tcgtcaaaat cgtcaagtcc gacgacgaat ggaaaaagct cctggcgacg
     1081 gaggcgtacc aagtgttaag acatgaagct accgagcgac catttacgaa caacatgcat
     1141 gacaataagc aggccggtat ttatcattgc gccggctgtg atcttcctgc ctattcatct
     1201 gaacataaat tcgacagcgg caccggctgg cccagctttt ggcagcccat cgatccgaag
     1261 gttgtcgaga cgcataccga tttcaagctg atttttccgc gtaccgaggt gcactgcgct
     1321 cgttgcgaag ggcaccaggg acatatcttc aaagacggcc cgaaacccac aggcctacgc
     1381 tattgcatca acggtgcagc actgaaattc gtccctgcct cagcatgagg gtcctggctg
     1441 cctagcacag taccgtcgac cgctccgatt caacggcccg ctccccgagg cacgacactc
     1501 ccttgacgat gtgccgagaa tcgggcatat caccgaaaag cgatctcatt cagggaagga
     1561 accgaaacca tggcacatgt cggctttgtt ggtgtcggac gcatgggcgc caatatggct
     1621 cgccgcctgc aggataaggg atatcgcatc actgcggttc acgaccgcaa tcagcaggcg
     1681 gccgatgagc tcagtcgcga attaggatgt gaggcagtgg aaactccggc acgcgtcgct
     1741 gagttgtccg aggtcgttgt caccgttgtg tccgatgatg cagccatgca cgacatctac
     1801 cgcgaggggg cgtccaacag cctcttagcg catgccaagg gccgtctttt tatcaattgc
     1861 gcgacggtga gcccggatgt tcacattgct acggaacagg ttgtcgaacg acacgaaggc
     1921 gccagcctgg aagcttgcat ggccagcagc atcacccaag cccgccaagg caccctctac
     1981 ctcatgtgcg ccggcaagga gtctgtcttc gagcgcgcca gaccgatgct tgaagtcatg
     2041 agtgcacacc ttcgttatat cggccgaacg ggagaggcgg ccaaggtgaa ggcgctcgtt
     2101 aatatggtta tgaattgcaa tacggcggcg ctggccgaag ggcttggcct aggtgccgct
     2161 cttggagtgg atcttacgat gttgcgagat gtgttcagcc agaccggcgc cgcctctcgc
     2221 gtcttggaaa ccgacggcca agacatgcag gcccgagccc acgactgcta tttctctgct
     2281 gcccatgcca ccaaggattc cggcattgcg ctcggactcg ctgcgaccgt gggattaact
     2341 ttgcctctgg ccgaagcgac acaccggcaa tatcaacggc tggtcgctgc tggcggaggc
     2401 gatttggaca aatccgctat tgccgagctc accttcaaag ggcgtcatca atagggcctt
     2461 gaccttccct tcatcaaccc ctgtcacggt aggatagcaa taatcctgac cggccctcct
     2521 gtcccgcctc gaatcttgat gggcagagcg acgagaatgg ctcccttcgg cgggaccttt
     2581 tcgagattgg cgacgttttc aagaccgtaa agattggcac cgtttatgat ctggtgcacg
     2641 atgaaatcgc gggaggagcc atagtcaatg ctcggcgtgt cgatgccgat tccatcgaca
     2701 tgccgctctt tcacaagaaa atcggcagct tccttcgaga aacccggaaa atgcaacgtc
     2761 ttcggatccg atggtgtcga acttccgaga tagcggaccg gatccggcca tctttttccc
     2821 cacccggtca gcatcaagac cactgccccc tgaacaatcg gtccatgccg ggcttcccag
     2881 gtttcgagat cccgcatcga gagtcgatag tcggcgtttt cttccaccgc tgaacggaca
     2941 tcgatgacga cggccggcgc gatgagcttc tgtacgggga tctcgtccac ggccagccgg
     3001 ccttccgcaa aatgaatggg cgcgtcgata tgtgtgccgc cgtgttcaga catggagaaa
     3061 tcacctgagg cataccagta accattggca gtcctccccc aggccgcctt ctcccaagtg
     3121 aacggcctgt tcgtaggcca gaacaccgtc tgctcatcga gggcgtacgt aagatcaatc
     3181 agttttgttt catcaatccc cgcccaaccc ggctgcgcgg caagaaggac gatgagactc
     3241 aagattcccc gtttcagcaa catagttcgc tcctcatgcc atcctcctgg ctttcaattc
     3301 ctgcactgtg ctatctcagg cgatgcccat gccgaatgtc ttactgctca ttcctccgct
     3361 aacgcaactg aatacgccct acccttccac ggcctattta accggcttcc ttcgttcgca
     3421 gggtgtcctc tgcgaacagg ccgatctcgg catcgagatg gtcctccgtg tattcagcag
     3481 ggaaggcctg cggcaggtgt tttatcagct taagcccaga aggcggtcgc tccatcctgt
     3541 ggctcaggag atgatcgcca atcaaaccgc catcctggat catatcgatg ccgtggtggc
     3601 ctatcttcaa gggaaagctc cggaactcac gaagtcattt gcccgacggg cgtttcttcc
     3661 tgaagggcct cgttttagga tgcagcgcag ctggtctcga agcacctcaa ctgaagaccg
     3721 ggcgaagcac gcggcgacat tatttctgga ggacctgacg gatctggttc agggaaccat
     3781 cacgccccat tttgcgctca accgctatgc ggaacacatc gcacgatcgg ctgcctcatt
     3841 cgacagcatg ttggctgcgc ttcaagcagc gccagccctg accgatcaat tcatgctcga
     3901 agccctgtgg gcgcatgtgg agcgcgtgaa ccccttgctc gtcggcctgt cggtgccctt
     3961 tcccgggaac ctctacggtg cctttcgcat cgcccaatcg ctcaagcaga acaggcctga
     4021 catcttgatt gcccttggcg gaggttatgt gaacaccgag ctccgtcgtc tgtccgacgt
     4081 tcgtgtgttc gattttgtcg actacgtcac gttggatgac ggcgagcggc ccctactctg
     4141 cctactcgag catctgtccg gtaagcggcc cgtgcaagcg ctcagtcgga cgtattaccg
     4201 tgacaacagt cgagtggtgt ttgccgatga tgtgacgctt ggcaacattg cgatgaatga
     4261 cgccggctgt ccgacgtatc ggggcctcat gctggatcgg tacctgtcaa ttcttgacag
     4321 caccaatccg atgcatcgct tgtggtcgga gggccattgg aacaagctga ccgttgcaca
     4381 cgggtgttat tggaaacaat gcacgttttg cgacgtaggg cttgactaca tcaatcgtta
     4441 cgaaatcact cccacagagc gtctcatcca caacatccag cagttggccg acgagacgaa
     4501 gcgtcttgcc tttcatttcg tggacgaagc tgcgccgccg atggcattaa aagcattggc
     4561 gatcgcgctc ctgcagcgca aaataggtat ttcgtggtgg ggcaacattc gatttgaaga
     4621 ggcgttcaca ccggatctct gccgcctctt agcggcgtcc ggttgcatcg ccgtcactgc
     4681 cggtctcgag gcggcctccg atcgactgct ggccaagatg aagaaaggca tcacggtcga
     4741 ccagacggcc cgcgtggccg ccgccttcag gcaggccgga attctcgtgc atgcctatct
     4801 catgtatggc tttccatcgg aaacggtgca ggaaaccatc gattcactcg aacgcgtccg
     4861 ccagttgttc gcggctgact tggttcagtc cgcattttgg catcgattca ccgtcactgc
     4921 gcacagtccg atcgggctcg atccgcgagg caacggcctt cgcattgtgg ggcctgagtt
     4981 tggcgggttc gccgaaaacg atctgctcca tgaggatgcg accggtccta ccccggaatg
     5041 gatcggtgca ggactgcgtc gatcgatgct caactttctg gaatcgcgag ggctggcgat
     5101 ggaggtcacg aaatggttcg atcatccgac tccaaggcca agagtgtcac cgacttggat
     5161 cagccgtgcc ctgggcaagc gcattccgga cgaggaattt gctgcggaac gccgctgtgt
     5221 ctggatcggc gaacaggctt gcgtcgaatc tggaaccggt catatcaagc tttcgattca
     5281 aggccggagg agggcgctga ccgctacgct tccatccagg caagcatcgt ggttgagcga
     5341 gctgctgaac gaagcgcttc cgcggcgaga tcgttatgcg tcgtatccgt catggcgtga
     5401 tgccaaactt gccttccccg gcagggcgac tgactttgcc tcgtttgtga gagatccgtc
     5461 ctggaggcgg atccggagtg cgggacttct cctggtgtaa cgatgcccca taaaccatcc
     5521 ccgcttgtcg ctaccagtcc ttcaggacaa gtttgccgat cattgtgcct gattccagct
     5581 tggcgtgcgc tttttttaga ttctccgcgt tgatgggatg cagggtctcg cggagaatgc
     5641 ccttcagagt accgcgatcg atccaatcgg ccactcgatt gagcaagcgg tgttgctcaa
     5701 tcatgtcggc cgtctggtac ttcggtcggg taaacatgaa ctcccacacg tgggtgatgc
     5761 tcttcgcttt caaggcctgt tgttgcaaga ggcccttgtt ctcgacgatc gtacagatct
     5821 tcccttgcgg agcaatcagc tcagccatac tgtcccagta gccgtcggtg tcggcacagt
     5881 tcaagatgaa gttgacgtct ccaaagccga ttccctcaag ctgaggcctc agcggccgcc
     5941 gatgatccag aacctcgtct gccccgagtt gcttgaccca ggcgatcgat tcgggacgtg
     6001 atgccgtggc caagaccgaa agtccagcaa tttttcccag ctgaatcgcc atcgaaccga
     6061 ccccgccgcc ggcgccgata atgaggaggg tgcggccctt cgaaccaccc tcgaagtcaa
     6121 ttccaagacg ctcttgtatg ccttcatacg cggtaatggc gactaacgga agggctgcag
     6181 ctgcttcggc tgcgagcgaa cgcggtctcc ttccgacaat gcgctcatcg accaactgat
     6241 attggctgtt gcttccaggc ctggaaatat cgccggcata atagacggag tcccccggcc
     6301 tgaagagcgt taccgcctgc ccgacagatt caaccactcc tgccgcatcc catccgagca
     6361 caagcggggt ctgcctatct ggtttgccag gcgcgcgcac tttcgtatcg acagggttta
     6421 ctgacagagc ctcgatgcgg acgagcaggt catgtccggt ggcctccggc aggggaatgt
     6481 cgaggtcgag caggctctcc gggtccgtaa ttgcaagatg tcgatacagg cccactgctc
     6541 gcatgcagct ctcctcttga ggagacagaa agggcgggaa gagatgggca tcccttcccg
     6601 cccttcagca agcttgttcc gttacttgtt cggctgtggt gtatagcgga ggtacggctt
     6661 caccgtcttg aagcccttcg gaaagagcgt cttcgcctca tcgttgctga cggccggggt
     6721 aatgatgcag tcttcccctt ctttccagtt ggccggagtc gctactttgt actttgacgt
     6781 cagttggagg gagtcgacga ctcgcaacaa ttcatcgaag ttgcggccac atgaagcagg
     6841 atacgtcaag gttaacttaa tcttcttgtc cggcccaatg acgaagaccg agcgtaccgt
     6901 catgttgtca agcgcgttgg gatggatcat gtcatacagc gccgcgacct tcttatccgg
     6961 atcggcgatg atgggataat tcatggtcgt gtgctgtgtc tcgttgatgt cgttgatcca
     7021 gcctttatga gaatccagtg gatccacact gacggcgatc actttgaccc cgcgcttctt
     7081 gaaatcaggc gtaattttcg ccacggtgcc gagctcggtg gtgcagacgg gggtgtaatc
     7141 ttttggatgc gaaaagagaa ttccccaact gttgccgagc cactcatgaa agttgatcgt
     7201 cccttccgtt gtctcagccg tgaaattcgg agcttcatct cctaatcgta gagccatgac
     7261 cgacctcctt agttatccaa gtccttaaga actcatgaac atggtacgat actgtccctt
     7321 ccgaaggaag tgcaagaggc aactcaccta tgagccggcc gtaatattca agcggctttg
     7381 caccttgcca tcatcggacg tggtcagatc aacatcggtc agaaacacac gctcctccgg
     7441 tagcttgcca tcgcccacga tcactgcttg cacctgttcg gcacgcgcgc gggcaagaga
     7501 ccgaagcgtg tcatcatcca ctcgaattga ctccgccagg ttacgcttca tctcttccag
     7561 ggtcggcggc tggacaggag catcaggtgg gcgaggctcc atcggctgct gactctggcg
     7621 ctgatcgaag agttttgtga tcaggcgtgc ctctgcactc agaggcaagt cagactcctg
     7681 cgtgttgccg ttctcctggc gccaccgact ccgcagcaag tcctgcaatc tctgaagcgc
     7741 cactgcctga cggtcgcgag ttggatcggc ggtgccggta atttcaaggc gcaagcctgg
     7801 ccgctcttcc agtcctttcg caagcgcatc gatctttttc atctcggatg gctttatggc
     7861 tgccgatccc ggctcaaagg tcaggtgctg caattcttct ccatccccac cgccgggaac
     7921 cagcttgccc ataagagcaa agggtgaggc gaccatcttg cttagaagat tgcccaaggt
     7981 ggcccagaca gccttcccgt atctgaaatc gggatccttg aggtctcctc gtattgggag
     8041 atcaatgtcg atcctgcctt gtcggtcctt gagcagggca atggccagtg gcacgggcaa
     8101 ggacgtggcg tcggggctgt cggtcttctc gccaaacgtg agttggtcaa ccgccacctt
     8161 gttttcggct tccagttgct tctgcgagac cttgtacacc aggtccaaga acagttttcc
     8221 tttcctgatt ggatagccga catacttgcc ggtatagggg gcggcagtgg tcagatcgac
     8281 attgtcgaac ttaatggtca aatcggtgaa ggtgtcttca gtcaaggggt taatggttcc
     8341 cgcgatcttc aggggagcca ccttgtcgat tttgccggaa agatccacct ccgccctagc
     8401 catttgtttt gacgagagac ctttcaccgt ccccgtcaaa tcgtagagtc ccgcctgcac
     8461 cgacggtgca atggattcat cctggaacgt ggccgtcccc ctgagcacct tgacggtctt
     8521 gatcgtaacg gaaggaggcg gggctttctt cgtgggggat gccgatcgct cagtacccat
     8581 cggctcgttc tttgccttgg aaccagatag cagtttcttg atgttcggca cgccgtcccg
     8641 atcgatcgct agatgcacgg tcggttggtt gatcccgacc tcctcgatcg tgacgctcgt
     8701 cggctcgaga gcgacggcga tctcgcgcag ctgtacctgc ttccaggagg ctaccggagc
     8761 gccgtcgtcc cggtcggcga tggccaacgc tttcactccg agattcccct tgaaggccat
     8821 cagagggcca ttcgggtgtt cagccgcaaa tttgagcgtt ccatccaaat caagcgcacc
     8881 agtatccaca gcgatacgcg catactgctc aacatatggt tggaaaggct gaatcgggat
     8941 atctttcagc gagacg
//
LOCUS       DASSCQ010000111         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1459151, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000111 DASSCQ010000000
VERSION     DASSCQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459151"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1336
                     /locus_tag="VFH05_07915"
     CDS             200..1336
                     /locus_tag="VFH05_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678178.1"
                     /translation="MPLFFLGLVGFTTAVPVQSLSSDQVQERPRERPTTEPVGTQYSI
                     PGASFLVVQRHTNPDASERALDEAAARTVVEALTFMLDHRKDYPRFDESMNKQALQEV
                     VIEPTVINQEGKAFPFLVARTSQPGQVRLLISAELLRKQGYVGHPEALVPALAKEFQW
                     VASKADTGAKIQPSSVRSDLAMVPIRTDEDIAALSAGERLNLLRALLGRYLHTVDEHN
                     SLHGHPHYEAGTNILIPPAQPDSTTKLYDIRVREALQKIVQDQWFMTHTPKAVRCLLN
                     GTIWNVAFVKIDQRDWATRTRVSSKTVVVGEASRTIQPATVLVNTFRLAAPDDSFYGD
                     SNGLPMGALSADQLARVIAKEIEQNIIEKSMRGHVAQDEQTMPK"
     gene            complement(1541..2086)
                     /locus_tag="VFH05_07920"
     CDS             complement(1541..2086)
                     /locus_tag="VFH05_07920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678179.1"
                     /translation="MPRIPLQASVSYLTPELLGKGLVIDVSREGLRIESQDPVHVGMR
                     LALVLYLSNDHEPVMIEDATVQWTLGTHFGVKFVKRSTNAEARLTNFFWTGIQEKCDS
                     LVNLMTEAVKASPLTESRDAQATPLEDAQRDLIRRTLEACRWVIGGASGAAKKLGMKR
                     TSLHYRMKKLGITRTTRGSGN"
     gene            complement(2307..>2792)
                     /locus_tag="VFH05_07925"
     CDS             complement(2307..>2792)
                     /locus_tag="VFH05_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678180.1"
                     /translation="GSGAQSWGLWPLDPGPRGVELSSYKRLKDAGGVAPARWKFDSTD
                     WWLEEHGLIMEQPVPLPPGKYLVTGAREVTAVLTIHAADKTGDRRWELDNGAVLYDVT
                     HLACRSARYTPVEVGGSCSPANAQKTAFPVAPGAAMPPVQGCTKRDYAVLIVIGVGVE
                     D"
BASE COUNT          612 a          805 c          783 g          592 t
ORIGIN      
        1 aggccccgac cttctgaaca tctcaccgcc atcactgaag agtatcgcgt ggctcagcta
       61 caaagctcct cccctgtttc cgacgtgaag ccggcggtat gagcgttgac gaaacccgta
      121 ttataatgga ggaggcgcta ctgccaaaac acatggacgt ggagacctgc ctgtgtggaa
      181 accttctgga atagcaagcg tgccactctt ttttttgggg ttggtcggtt ttacgacggc
      241 cgtaccagtc cagtcgctat caagcgacca agtccaagaa cgccctcgtg aacggccgac
      301 gaccgaaccg gtaggcactc agtattcaat accgggtgcc tcctttctcg tcgtgcagcg
      361 tcatacgaat cccgatgcgt ctgaacgggc actcgatgaa gcagccgctc gaacagtggt
      421 ggaggccttg acgttcatgc tggatcatcg aaaggattac cctcgatttg atgaaagcat
      481 gaataagcag gctctacagg aggtagttat tgagccgact gtgatcaatc aggagggaaa
      541 ggcgtttccc ttcctggtcg ccaggacctc acagccaggc caggtacgcc tgctcattag
      601 tgcagaattg ctccgcaaac agggatacgt tggacatcct gaggcgctcg tcccggcgct
      661 ggcgaaagag ttccaatggg tcgcgagcaa agcagatacc ggagcgaaga tccagccgtc
      721 ttcggtgcgc agtgatcttg ctatggtgcc gatccgtacg gacgaggaca tcgctgcgct
      781 gtcagccggg gagcgtctca atctgcttcg tgcgctcttg ggacggtatc tgcataccgt
      841 ggatgagcac aacagtctcc atggccaccc gcattacgaa gcggggacga acatcttgat
      901 tcctccggca cagccagatt ccaccacaaa gctgtacgat attcgggtcc gcgaggcctt
      961 acaaaaaatc gtgcaggacc aatggttcat gacgcacacc ccaaaagcgg tccgctgctt
     1021 gctgaacggc acgatatgga acgtcgcttt tgtaaagatt gatcaacgtg attgggccac
     1081 acgtacccgt gtgtcgtcga agacggtggt ggttggagaa gccagtcgca cgattcaacc
     1141 ggccacggtc ctagtgaata cattccggct ggcagcgcca gatgattcct tctatgggga
     1201 ttcgaatggc cttcccatgg gcgccctgtc agcagaccag ctcgcgcgag tcatcgcgaa
     1261 ggaaattgaa cagaatatca ttgagaaatc tatgagaggt cacgttgccc aagacgagca
     1321 gaccatgccg aagtgaacgc tggaacgaca aaccctcccc cggacagtcc gcattccgat
     1381 attccgttga ggtgattctg ccctgacgga tgtccgtgct ccccggtttg aaatccgaac
     1441 actcattctt tacccaacat ggggccgatg gggccgggat gtactgtcct cgtagcagtg
     1501 gtaacagaca ggtggagcca gacgggagat tttgcaaaac ctaattgccg gatccgcgtg
     1561 tggttctggt aatgcctaac ttcttcatcc tgtagtgaag agaggtgcgc ttcataccta
     1621 gctttttggc ggcacctgac gcgccgccga tgacccatcg gcatgcttcg agcgtccgac
     1681 ggatcagatc gcgctgggca tcttcaaggg gcgtggcttg ggcatcccta ctctcggtga
     1741 gaggcgatgc cttgaccgcc tcagtcatga gattcactaa ggagtcgcat ttttcctgaa
     1801 ttcccgtcca aaaaaagttg gtaagcctgg cttcggcgtt cgtgctccgt ttgacaaact
     1861 tgaccccaaa gtgtgttccg agagtccatt ggacggtggc atcttcgatc atgacaggct
     1921 cgtggtcgtt cgagaggtag aggaccagcg ccagtcgcat cccgacatga accggatctt
     1981 ggctttcaat tcgaagccct tctcgtgaga catcgatgac cagtccctta cccagtagtt
     2041 caggggtgag atagctcacc gaagcctgaa gagggatgcg gggcacgcat ctcccggtcc
     2101 atgccttctc gtttacgacc atatgttcgc ctgtgctgaa gacaataacg acttggccgt
     2161 cagtaccctt caactatgcg ccgatggaga tggtctgtca cgtgtttcag cacaagttgc
     2221 aatcaactaa gagaggagga aaagaagtgg tgagcggctg gggctccagt ccaggccttc
     2281 gcagtaaaga taacttagac cagcgttcag tcttccacgc ccacgccgat cacgatgaga
     2341 accgcatagt cccgcttcgt gcagccctgg accggaggca tcgctgcccc tggtgcaacc
     2401 ggaaacgccg tcttctgcgc atttgccggc gagcacgaac caccaacctc gaccggtgta
     2461 tagcgcgcgg accggcaagc caggtgcgtt acgtcgtaga gcaccgcgcc gttatcgagt
     2521 tcccagcgcc tgtcgccagt cttgtcggcg gcgtggatgg tcagcacggc cgtcacttct
     2581 cgggcgcccg tcaccaggta tttgcccgga ggaagcggaa ctggctgctc catgatcaag
     2641 ccgtgttcct ccagccacca gtcggtgctg tcgaatttcc agcgcgccgg ggcgacgcca
     2701 ccagcatcct tcaaccgttt atagctgctc agctcgacgc cccgaggacc cggatccagt
     2761 ggccacagcc cccacgattg agcgccgctc cc
//
LOCUS       DASSCQ010000112         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_504628, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000112 DASSCQ010000000
VERSION     DASSCQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504628"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1015)
                     /locus_tag="VFH05_07930"
     CDS             complement(11..1015)
                     /locus_tag="VFH05_07930"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET6678181.1"
                     /translation="MAANTPIFPMEALLVGHLPEGHQWRYEPKWDGFRCLAYRRNKSV
                     TLRSKSGKPLERYFPDIVGILQKLAAKRFVLDGELLVKGENGYSFSELQMRLHPAASR
                     VNMLSQKQPATFVLFDILETDQGINILSAPLSERRRNLERFWEKYCRKEKAVALSPQT
                     NSITEAMKWLDTSGWYVDGIVAKNSRDAYIPGERVMHKYKRIRTADCVVGGFRYGRDS
                     KEVGSLLLGLYDKRGQLHHVGFTSGIAKADKPSLTKQLEQLIQEPGFTGNAPGAPSRW
                     STERSTQWKPLMPKLVVEVSFDHVTDRRFRHGTRLIRFRPDKSPRQCTMEQLIDNPTK
                     "
     gene            complement(1027..1968)
                     /gene="ligD"
                     /locus_tag="VFH05_07935"
     CDS             complement(1027..1968)
                     /gene="ligD"
                     /locus_tag="VFH05_07935"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6678182.1"
                     /translation="MWPESIPLSQCDSKGPAVKDTPIADQHLVLHGVKITHPDRLVFE
                     EAKITKGDVARYYAAVAPSLLREIRNRPITVVRCPSGVKANCFYQRNVGFGLGTHVYP
                     FLWKYKGRSYSYIYVKDVMGIMQMIQMGVIEIHPWGATIGNIHRPDRMIFDLDPDPSI
                     PFSKVKHAAREIRKRLQKAGLESELKCTGGKGLHLVVPLIPARSWHEIKAWASAFAHT
                     MVTESPDKYVATITKSKRKGKILIDFFRNDYTATSIASYSLRARAGAPVSVPLEWREL
                     TRLESASQFGMDVVLKRIQRQQSQEALPKQRLPKLTT"
     gene            2156..2539
                     /locus_tag="VFH05_07940"
     CDS             2156..2539
                     /locus_tag="VFH05_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678183.1"
                     /translation="MKQAYKRLMACSIAGVGVLLAVIASADMGKTAPALPAPSGSKAE
                     SAIKEGMIHYEQGHWDVAKKHFEKAVQADDQSAEAHYDLALALDKSGDHQAAIQHFKA
                     AQQYGKDNPEIQNSGILQAHLKAKH"
     gene            complement(2606..>2898)
                     /locus_tag="VFH05_07945"
     CDS             complement(2606..>2898)
                     /locus_tag="VFH05_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678184.1"
                     /translation="KTRKVAMNGQIWLIATLLSAVAGCESYSWSVYNNPLIPDVKQGQ
                     DCRVLVFGLGGSVDPTGREAMRLGDITRVRNTEFRVNTVQGVGNECVIAHGE"
BASE COUNT          649 a          797 c          758 g          694 t
ORIGIN      
        1 gtgtccacgt tcatttcgtc ggattatcga tgagctgttc catcgtacat tgtctaggcg
       61 acttgtccgg ccgaaacctg atcaatctgg tgccgtggcg aaagcgccgg tctgtgacat
      121 ggtcaaaact gacttcgacc acgagttttg gcattagcgg cttccattga gtcgatcgct
      181 ccgtcgacca tcggctcgga gctccgggag cattcccagt gaagcccggc tcttgaataa
      241 gctgttccaa ttgtttcgtc agggacggct tgtcggcctt tgcaatgccc gaggtaaagc
      301 cgacatggtg aagctgcccc cgtttgtcgt agaggccgag aagcagagac cccacttcct
      361 ttgaatcacg gccgtaacga aaaccgccta caacgcaatc cgccgtgcgg atacgcttat
      421 atttgtgcat cacccgttcg cccggaatgt aggcatcgcg actattcttg gcgacaatgc
      481 catcaacata ccaaccggaa gtgtcgagcc atttcatggc ttccgtgatg gagttggtct
      541 gtggcgatag ggcgacagct ttttcttttc ggcagtactt ctcccagaac cgttcaagat
      601 tccggcggcg ttccgagaga ggcgctgaca ggatattgat gccttggtct gtctcaagga
      661 tgtcgaagag cacaaaagtc gcgggttgtt tctgcgaaag catgttgacc cgactcgccg
      721 ccgggtgtag gcgcatctgg agttccgaaa acgaatagcc attctcacct ttgaccaata
      781 attctccgtc gaggacaaaa cgcttggccg ccagcttttg aagaatgcca acaatgtccg
      841 gaaagtatct ttccaacggc tttccggatt ttgaccgcaa cgtcacagat ttgttccgtc
      901 tgtacgcaag gcaacgaaat ccgtcccact tcggttcata tcgccattgg tggccctcag
      961 gcaaatgccc gacgagcaac gcttccattg ggaatattgg cgtgtttgcg gccatgcatc
     1021 catccttcag gtcgtcagct tcggcagccg ctgcttcggc agtgcctcct gtgattgctg
     1081 ccgttgtatc cttttcagga caacatccat gccgaattgg cttgcgcttt ccagtctcgt
     1141 caactcgcgc cactccaggg gcacggagac gggtgctccg gcgcgtgcac gcaatgaata
     1201 gcttgcaatg ctcgtagccg tatagtcgtt acggaagaag tcaatgagga ttttcccttt
     1261 gcgcttggat ttggtgatgg tcgcgacgta cttgtccggc gactctgtta ccattgtgtg
     1321 ggcaaaagcc gaggcccagg ctttgatttc atgccagctc ctggcaggta tcaatggcac
     1381 cacgagatgc aggcctttgc cgccggtgca cttcaattca ctttcaagtc cggccttttg
     1441 tagccgcttt ctaatttcac gggctgcgtg tttgaccttc gagaacggta tgctcggatc
     1501 cgggtcgaga tcaaaaatca tccggtcagg cctatggatg ttgccgatcg tcgcacccca
     1561 aggatgaatc tcaatgaccc ccatctggat catctgcatg atccccatga catccttcac
     1621 atagatgtaa ctgtagctgc gacctttata cttccaaaga aatggataga cgtgcgtgcc
     1681 taaaccaaag cccacattcc gctgataaaa acaattagcc ttgacgccgc ttggacatct
     1741 caccacagta atagggcgat ttcgtatttc ccgtagtagg gaaggagcga ccgccgcata
     1801 atatcgagcc acatcgcctt tcgtaatctt tgcctcttcg aatacaagac ggtcgggatg
     1861 tgtgatcttc acaccatgca gaacaagatg ctggtcagcg atcggggtgt ccttcactgc
     1921 agggcctttg ctgtcgcatt gagagagtgg aattgactca ggccacatgc cgccttttca
     1981 tctacggcga caccgtcaac gagccttatt atgcaccgaa cgtgatgtcc attgatgcag
     2041 agcagtccac attcagccgc cactctcgtg cagcaagggg ctcacccagt ttgaggcagc
     2101 atcgaagtgg tgttagacat cacgtaattc actcgtaatt cactatggag gttctatgaa
     2161 gcaagcttac aaacgtctta tggcctgctc aatagcgggt gttggggtgc tgttggccgt
     2221 catcgcttcg gccgatatgg gtaagactgc cccggcgctc ccggctccct caggctcgaa
     2281 agcggaatcc gcaatcaagg aaggcatgat ccactatgag caagggcact gggacgtcgc
     2341 aaaaaagcat ttcgagaagg cggtgcaggc tgatgatcag tccgccgagg cccactatga
     2401 cttggcactc gcgctggata aatctggaga tcaccaggcg gcgatccagc acttcaaggc
     2461 cgctcaacaa tatggaaaag acaatcccga gatccagaac tccggcatcc tgcaggccca
     2521 tttgaaggcg aagcactaga gtaagaatct acgcgaggct tgccggggcg ccttgctccg
     2581 gggagaagga gaggtgacga gtcggttact ctccgtgcgc gatgacacac tcatttccca
     2641 cgccttgaac cgtgttcacg cgaaactcgg tgttccgaac tcttgttatg tccccgagac
     2701 gcatggcttc acgtccggtt ggatcaactg agccaccaag gccgaatacc agaactcggc
     2761 agtcctgacc ttgtttgaca tccggaatca aggggttgtt gtacacagac caggaatacg
     2821 attcgcaccc agcgaccgct gataagagcg tggcgatcag ccaaatttgt ccgttcatag
     2881 ccacctttct tgttttga
//
LOCUS       DASSCQ010000113        21042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1077479, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000113 DASSCQ010000000
VERSION     DASSCQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 21042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21042
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077479"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="VFH05_07950"
     CDS             <1..1569
                     /locus_tag="VFH05_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678185.1"
                     /translation="SYTHVSYVDLKPKVTDVRIFSPEAACGGGATPTASGCIHPDGWG
                     TILILGMRFGGSCGSCTAVSGTNNGGPALSVTAKFDGVATTTRTFYSAYIVLDITDPD
                     SNPTVLSVYSSSTLGLTTSYPTVARMSPAGDGKSDHTNAKFFMVAGSGVQGYNGRAVT
                     GAKLFAVQLVLPGTAPTVTILPVGADTYGGFMADPITFDRDLDFRSDAIYVGRTIAPD
                     AGGAAALGYWWGKFYRLTMGTCSSAPCTTSTWGVLSSGNRIPTEMVMQVPIGGTPKYL
                     GPVTAGSTVTLDDSGNTWVFFGTGRFFAAADKADQHANYLVGVKDSVLRSGGCVQTDA
                     VNCTNQNLLDVSSAQICVSCASGNQVQGVGSVTTFSGLMDQIRGNTSLGISAKDGWVI
                     QLNPNTGSTTLGAERSLVNPTLIGGAIFFPTFTPNNDICVSTGNSSLYAMYYLTGTGY
                     TDPIIGLDANGVSQRSVGIGEGLASSVAIQIGAQPTGMSGFYQSSNSVMSKVSPKAPI
                     SLWSQYISWISNRE"
     gene            1837..2655
                     /locus_tag="VFH05_07955"
     CDS             1837..2655
                     /locus_tag="VFH05_07955"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET6678186.1"
                     /translation="MKTVSILPNPLNLSGPIGVRVEAQDLDQQTMVFRYRWLVNGHVM
                     SGQTLSTLQAHLLKRGDQVAVEVIPFDGITEGRPFQSVPLTVPNTAPIISNLSVDLDH
                     DVQGRRLLAKVDVADPDHDSITLTYRWRKNEKLLKEGEDNALDLADLTAKDTVNVEVT
                     ASDGTPHGSTTASERFTLTNSSPSIVSSPMASPRGDRYEYLVRATDPDGDPLTYALEV
                     SPPGMTIEAETGQIHWMISPEMTGSQRVRVVARDPQGGFATQDFDLSLNKPTKS"
     gene            complement(2745..5735)
                     /locus_tag="VFH05_07960"
     CDS             complement(2745..5735)
                     /locus_tag="VFH05_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HET6678187.1"
                     /translation="MLTLHASEPEVVIRRDANGQWHLPLLEPHQTETGDSTSPQRWML
                     TESKLTDGKLRILDANRLEHEGIGIHDVQVLFQSHPTRTQADLMLTGITDDGGDLHIA
                     GTLALQQHGQSNTAIPRQFDGILRFHQWDIAYWLKRTGQSAAIPTQMAAWRGNISAGL
                     HLVFPPDAKGFNVVVSDLNTDLGWLLMRGQIMVEGAGTDHPAYAVKLSTSPVSSKTFF
                     DNIPPSWIPKTIHAAVDEHELTGTVELESVELRGRIDVLRIPDEWQMAAKVIHARGSL
                     EKTHTLIRNVSGTVWLNSKHANITDFAGEVNGVHVTSSKLSVSDLDVRPIVDAHILAE
                     GPFEKVMAVVEHFSEGTGAHDALPTVSDATGTVHVGVHVNGPIIPRPNLRMISASLDL
                     QDIGAHVARAMLIGQVNGAFAADSRLMGIKHLSGVVQGIHFEAEGNIDVASPNRVNTL
                     TVNMSSDGTAIQKLLATYLPATSDVHIDGPAHSTMFLSGTAALVHCRGTLDVTDAEIS
                     VPSLLNKKQGIPGRIEWEGKLFDGKRVVVDHLRLVVPHGEIRAAGQLDLGHTPKFHVN
                     VNAGPLSLRALQAIGVESPISEGMLEASALISGEGTDWKLWVPSGWVSIHRAVVAFPG
                     LDEQLRELSGRAQLSKGGLLVNELLFTMGESDFKLTGMVEDWRTHPRATFMVESSQLN
                     VSDFVSSKTVDPDTKESHVQEWFQSKKAAITFLVKQLRYDRLVLKTVSGEITVDQHNA
                     KLKGLRGQTQEGVLSGLVEARFGARDQMEVAAEMSVNGIPAQQLLSATEDNNELLQGN
                     LSLNGILQARINATSPLRHTLSTGPGGIVVKVTKGRLQQDPVLTKVLKILNLPSVLFG
                     PVDFGVRGIPFDSLSARVVGSNGVFSSKDIVLDSPVIKVTGAGSADVKDNGLDLAVAV
                     SPVAAYSDLIGKIPLFGQLFGEDHQGLTTAVFEAKGTLQNPDVAYLPLESLARGLTGY
                     PRLAIDVLVNTLKLPPTALAYATE"
     gene            complement(6499..7716)
                     /locus_tag="VFH05_07965"
     CDS             complement(6499..7716)
                     /locus_tag="VFH05_07965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET6678188.1"
                     /translation="MSITLKSERLETEKRQAVIVSAVRTPMGSFNGVFSSVPATKLGS
                     IAIVEALKRVHVLPEHVDDVLMGCVLSAGLGQAPARQASIGAGIPHSIGATTVNKVCG
                     SSIQTVMMASQTIALGEANIVVAGGMENMTRAPYLLEKARQGYRLGHAEMVDSVIKDG
                     LWDVYNNFHMGNAGELCAAKYRLTRKELDDYALESYQRAQYAIDAGAFKPEIAPVDVP
                     QRKGASLTIIDDEEPKRIDLKKLREFKPVFQENGLLTVGNSPSCNDGAAALVVMAQEE
                     AVRRKLVPMARIVGYAGAALAPEWLSIAPVEAIKLVLEKTSLSLGDIDLFEINEAFSA
                     VSLAINRELGLDPEKVNVHGGAIALGHPIGATGARIITTLLYAMEARGARRGLASLCI
                     GGGEALAIIVERI"
     gene            complement(7679..9646)
                     /locus_tag="VFH05_07970"
     CDS             complement(7679..9646)
                     /locus_tag="VFH05_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6678189.1"
                     /translation="MSKPDIFQGSVAIAETARDRASYTGFLAGLFESDVRWKLFTSIE
                     IPPMSSLALDFLHRFRTTLLPLDPERVDSEGELPEPLLKEFAQLGAFGIKIPRQYGGL
                     ECTQAEYQKVATLCGSVDASLTVLLSAAQSIGVPEPLRLFGTDKQKAKYLPRLARGEI
                     SGFALTERNVGCDISKVETYAVRILEQGKTVGYRLTGEKFFITNSAKRDGEFLSSMLV
                     VIARIVDRPEEVNDPRSQKRYGAFIVETQWPGCSVTRLRFEGVRAIYNGIPTFRDVYV
                     PVENRLGGEEDGLRIALATLTVGRLTLPAACLGGLKQCLSLMRWWGQSRVQWNRPIGE
                     HNLIGEKLCRTAAYTLALEAVMAFCGAWANKKGDLRLESAAAKIIGSEWYWEAVNELF
                     QVRGGRGFMTAEAQRKSGEPSLPVMRMLRDARINLVWEGTSEILRVWMAREALSPYID
                     RGLAFLQGTWTDKLAAPLYYAHMSLRSCLPFLRPTPAPFGDENSRWVRFIEAAARRIT
                     RSTLKATLRHQQRLHNKQLLLQHLVNDSLLLFPMAATLWYANQPEMRTKPGIQDLVDY
                     FCQDMADRLCPPSSLAGRIRRHKKDSVVYRLAKMLMSGQYDWLEEGIIPVLRNDTAAR
                     NMNPSNPTDLSSDLSAGQSNVDHTQVRTPGD"
     gene            9877..11202
                     /locus_tag="VFH05_07975"
     CDS             9877..11202
                     /locus_tag="VFH05_07975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET6678190.1"
                     /translation="MRNVVVVDGFRTPLCKEGTDFRETDAEVLGAWVVREIITRLHRW
                     NLPLTTIDCVLGSNVATPMHAVNPTRVAAVTGGLPATIPADTVAGKNCGSGVTALYYS
                     GLRIRSGDADTVLAIGMEAMSRIPLVYDRTVADLLLQYGKARTLRERTAGVLALIPKL
                     LNLKRYPPRVGLISGLTDPMCDLIMGLTAENISKDPALGITREDQDAFAVRSHRNAAR
                     AWKNGLLADEVVPMYVPERSAYVSRDNGIREDASAQTFRDVKPVFDRHHGSVTPANAS
                     QITDAAAAMLLMEESKAKSLGLPILGYLKEYADFGYEPSCMGLAPVGAIATVLSRARL
                     TLKDFAVCEINEAFASVVLGIMRILDSSALMERKFGRYGWTERLGEIPYDDLNPHGGA
                     IALGHPVGVSGLRLAMTALYELKRRNAERALISMCVGGGQGVAMILERK"
     gene            11205..13391
                     /locus_tag="VFH05_07980"
     CDS             11205..13391
                     /locus_tag="VFH05_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET6678191.1"
                     /translation="MSHDGSQSFSLPATLKHFKTAILDDGMLLVGFDYQGKSVNVLNV
                     ESMSEWQHIVQAAERSTTITGVLLMSLKEGNFCAGADLEQMHEAQRQGSFRSMEQLVE
                     IAHQIFDAMDRSAKPFVVAVEGSCLGGGLELALACHRRIASTHPKTCFALPEVKLGIL
                     PGFGGTQRLPRLIGLPAALDLITAGRMVFPRQALRMGLIDGMVSSVPSRMRTLEAIQT
                     ETLIQAAISQARLLRSAPARQRPLRLSQRLLGGPLVRVLLCGYARHQVKKRVRHFYPA
                     PLRAVDAMQRGMGMSVLKGSLIAEKPLLMELMASPVSRHLVSLFLAGEDVKRKAAPVP
                     DAVTRVGVLGAGLMGSQIAGQLAEKGYAVVLRDVAPSILAAAMGRILKAQTNQVRKKI
                     ILPSELRYRLLRIAPTTTIKDVSPAPLVIEAVSEKLDVKRSVLAEFESEAGPDAIFAT
                     NTSSYTLTDIALKAVHPQRCVGLHFFNPVAKMQLVEVVRAPFTSDQALAQACGIAKRL
                     GKFPLVVRDGPGFLVNRILSRYLAEAVILVGEGVPIRRIDTIAKDFGMAVDSGHPMGP
                     LELIDLIGLPVAMHVLASLAVLGSRIESRDALLRNLLADTRTPVPFWKSGVENRQALD
                     VIADYRRTYPSEARPVSDEILHQRLFLPMVDEAVRCLHDGIVEQAWEVDFALTYGIGF
                     PAFRGGLLTWARQTMTPEQIARKLDELSHKYGKRFEPFPGLSHGGW"
     gene            13427..15154
                     /locus_tag="VFH05_07985"
     CDS             13427..15154
                     /locus_tag="VFH05_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6678192.1"
                     /translation="MHEQPGVHYPEGVPRELPLCSETLVDMLARSARRFSRRPALYFF
                     GKTVSYRECHDLVERFATGLATRGIRKGDRVALCLPNSPQAVIAYFGILRAGGIVVAC
                     NPTYTEHELSHQLRDAGARAIVVFDQMYQKIEHLNLELIIVTRITDFMTTIARYMYHH
                     RNGHRSPEIPIGGTTLWFHDVLHQAIPVEPENLPVVVPDDIAVLQYTGGITGVSKGAE
                     LRHRNLTANIQQLISWFSPAERVESFLAALPLFHVFGMTVTQNICLAVGGCMVLVPDP
                     RNMPVLLTLLRKTQPTVITLVPAFVRKLLEHCDAKDFTATRFVICGGASLSYELLKKF
                     KERTGHMIVEGYGLSEASPVTHCNLPRGLSVKRSIGLPIANTESKIVDAAGQEVPDGE
                     VGELWVRGPQVMERYWNNPVETANVVKPGGWLATGDMARIDQDGFYYIVDRKKDMILS
                     TSGFNVYPSEIEHVLLLHPLVHEAAVIGVQVTDGCEAIKAFIVLKVETVSVDELLAHC
                     RHYLASYKMPKHIEFRTELPQSILGKMLRRVLRVEEGGRLRLNGETPHRINGQNLAGQ
                     NTSGCKAGQ"
     gene            complement(15075..16253)
                     /locus_tag="VFH05_07990"
     CDS             complement(15075..16253)
                     /locus_tag="VFH05_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6678193.1"
                     /translation="METYDVCIVGAGVVGCAIARELGHKFPRLRVVIFEQGAQAGQET
                     SSRNSGVLHSGIHENPNSLKARLAREGSALAVSYAVQRGIPLLKSGMVIAFSWQDVRR
                     GLWRDVSSLRRLWANAWKSNIRVSFLTPHRLKALEPNLCGCAGLLIPSVCVIDALAFV
                     QALRADAEDTGSAFAFNNRVVEIDADSTSYLVTTDRTKIRATCLINAAGLYADDIASL
                     ALRATKYAIRPLRGEYYEVISPEKRGLIGRLIYPALPRQATGKGIHFSPRPNGQLFLG
                     PNEFAVLDKTDYTSRKTPPDMFLETLQNFLPALNERDLRWAYSGIRPCVMTGQGRKSD
                     FIVFADRENPPLVNLIGIESPGLSAALALARHVTDLPCIQRCFGPRGSGHLSGAVSHR
                     "
     gene            16310..17527
                     /locus_tag="VFH05_07995"
     CDS             16310..17527
                     /locus_tag="VFH05_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6678194.1"
                     /translation="MSELSSKYGKILFVFCGGGCKAIIQAVAASELVKAGFTPSHIIS
                     SSAGSCNALGFVENPGPVGAENTIRIWEDYITSPEAIYEVHPFFREKLTRLLGVVPRA
                     TQDWGPSGSILHDLRRAMKFVPLILSFCTRMPLRVAGRTVSFIFRLMDTFESQHKSFR
                     RLVQCPEIQEAFDEVDKFFEFKRMKAFLDPFPLLARLGEQIDLKKVLTSSTVWHILTE
                     RYEDGATVIFSNKDPDVAMDGNDDARSVKSKYDLFYKRIRASMALYPLFEMVDMGGYR
                     YLDADLANPLPVEEAFSVGCDTVFFFLNVPKKSVRVDPHPLRDLLELNDLLRLNERFI
                     HHRVMEAQVKAKTLGVNLFVVRPDEIPSGLGLLEIDNKVIQSVKIHERNRMKEYLCNL
                     DTHNLRFVPLSDE"
     gene            17577..18254
                     /locus_tag="VFH05_08000"
     CDS             17577..18254
                     /locus_tag="VFH05_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET6678195.1"
                     /translation="MLKIGHRGAAGHAPENTLAAIRKGIALGVDFVEIDVRCTADGAL
                     VVLHDSTVNRTTNGKGPIDRLSFRDVKALDAGNGESIPTLEEALRVLAGRTGLMVELK
                     VKGISRKTVEVVQTCRFKEPVIYASFLHEELGIIRSIDAQASIMPLFGRLPRAPVVRA
                     MKYSPSHVGLRHDTISRRLVEAFHDQDMLVCVYTADRKRDIERACSAGVDGIISNVPD
                     RVDNVPV"
     gene            18472..18657
                     /locus_tag="VFH05_08005"
     CDS             18472..18657
                     /locus_tag="VFH05_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET6678196.1"
                     /translation="MLRWSAVFLVIAIIAAALGYGGIAADAAEIAKVLFFIFLGIFVV
                     TLILGLIAGQKLFGQRR"
     gene            complement(18878..19075)
                     /locus_tag="VFH05_08010"
     CDS             complement(18878..19075)
                     /locus_tag="VFH05_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678197.1"
                     /translation="MNLRREIQAYTRACEHLLSLDQQLTDEERDLLEYYLSELSRKYL
                     SEKPTVRLRYPEAAAVEPSAR"
     gene            19254..19460
                     /locus_tag="VFH05_08015"
     CDS             19254..19460
                     /locus_tag="VFH05_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678198.1"
                     /translation="MNKSGEMEPTVLHNFYRATQSLREAMESDLPLNDLERISLENYI
                     ALLQMTYIEWKRRNCQPPAYRKAA"
     gene            19697..19879
                     /locus_tag="VFH05_08020"
     CDS             19697..19879
                     /locus_tag="VFH05_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678199.1"
                     /translation="MDCPKCNGLMDLERFSDFFLVFYAWKCINCGAVIDKTIAMNRRK
                     SLAAKQELTKVCVEST"
     gene            complement(20736..>21042)
                     /locus_tag="VFH05_08025"
     CDS             complement(20736..>21042)
                     /locus_tag="VFH05_08025"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HET6678200.1"
                     /translation="WIRKIYEYSLTLALFLLFIISFLLHAVAGRHVFNEEQALHGGHA
                     VTLGEYIMVSQFWFESLQNWQSEFLSVAVLIILSIFLRQKGSPQSKPVSHPHDETGD"
BASE COUNT         4644 a         5755 c         5878 g         4765 t
ORIGIN      
        1 agctataccc acgtgtccta tgtggatctg aagccgaaag tgactgacgt tcggattttc
       61 tctccggagg ccgcatgtgg aggtggtgcg acacctacgg cctcgggttg catccatccg
      121 gacgggtggg gcacgatttt gatcctcggc atgcgcttcg gcggcagctg cgggtcctgt
      181 acggctgtgt ctggtaccaa caatggggga ccggctctgt ccgtcaccgc aaaattcgat
      241 ggcgtggcga ccacgactcg gacgttttac agcgcgtata tcgtgttgga tatcacggat
      301 cctgactcca acccgacggt gctgtccgtc tattcgtcgt ccacactcgg attgacgacg
      361 agctatccga ccgtggctcg gatgagtccc gcaggcgacg ggaaaagcga ccatacgaat
      421 gccaagttct ttatggtcgc tggctccgga gtccagggct ataacgggag agcggtcaca
      481 ggtgccaagc tctttgccgt tcagctggtg ttgcctggga cagctccgac tgtgaccatt
      541 ctgccggtcg gcgccgacac ctatggtgga ttcatggccg atccgatcac gttcgaccgt
      601 gatttggatt tccgctcgga tgccatctac gttggccgga cgattgcgcc ggacgcagga
      661 ggcgcagccg ccctcgggta ttggtggggc aaattctatc gtctcacgat gggaacctgc
      721 agttctgccc cctgcacgac gtcaacctgg ggagtgctct cctccggaaa ccgcataccg
      781 actgaaatgg tgatgcaggt gccgatcgga ggaacgccaa agtatcttgg accggtcacg
      841 gccgggtcca cagtgacgct tgacgactcc ggcaatacat gggtgttttt tggcacggga
      901 cgcttctttg ccgcggccga caaagccgat caacacgcca attatctggt aggagtcaag
      961 gattcggttc ttcgatcagg cggctgtgtc cagacggatg cagtcaactg taccaatcaa
     1021 aatttgttag atgtgtccag tgcgcagatc tgtgtgtcgt gtgcgtcggg caaccaggtc
     1081 caaggggtcg ggtccgtgac cacgttttcc ggcctcatgg atcagatacg cgggaatact
     1141 tcactgggta ttagcgcaaa ggacggatgg gtaatccagc tcaaccccaa tactggttca
     1201 acgacgctgg gagctgagcg atctctggtc aaccccacat tgattggagg cgctatcttc
     1261 ttcccaacct tcacaccgaa taatgatatc tgcgtgtcaa ccggaaacag ctccctgtac
     1321 gcgatgtact acctcacggg aaccgggtat accgatccga tcatcggatt ggatgccaac
     1381 ggagtgtccc agcggagtgt cggaatcggt gagggtttgg cctcatctgt cgcgatacag
     1441 atcggtgctc agccgacggg aatgtctggc ttctatcagt ccagcaactc cgtgatgagc
     1501 aaggtgtcgc cgaaggcgcc gatatccctg tggagtcagt acatctcctg gatcagtaac
     1561 cgggaataaa cagagccgat gagaaccgac tggccgtcgc agggtagcat tgtacgtcct
     1621 ctagaacgaa gcgggcgctt gcgtacgccg ccctcagccc gggatcattc agtcgcgccc
     1681 gcttcagcag tcggagacca aagacgtcgt gtcttctggc tcgtcgggat cgtaggggct
     1741 ctgttcatag cttcagcgct cctcgggggt ggactgtcgc gtgataagaa cgagagggca
     1801 ggccaagctg taggcacatc gaatcactct cccgtcgtga agacggtatc cattctgcca
     1861 aatccgctga atctgtcagg gccaatcggc gtcagagtcg aagcgcagga tcttgaccaa
     1921 cagactatgg tgttccgcta tcgttggctg gtcaatggcc acgtcatgag tggtcaaacc
     1981 ctgagcacac tacaggctca cttgctcaaa cgcggggacc aagtcgctgt tgaggtcatc
     2041 ccttttgatg gaatcaccga ggggagaccg tttcaatccg tgccgctgac cgtcccgaat
     2101 accgctccaa tcatctctaa cctttcggtt gatttagatc atgacgttca aggccgtcgg
     2161 ctcttggcca aagtcgatgt tgccgacccc gatcacgact cgatcacgct cacctatcga
     2221 tggcggaaaa acgagaagct cctgaaggaa ggggaggaca atgcgctgga ccttgcggat
     2281 ctgacggcga aggacaccgt gaacgtggag gtgacggcat cggacggtac accacacgga
     2341 agcaccacgg cctcggaacg attcacgttg accaattcgt ccccatccat cgtatcaagt
     2401 ccgatggcat cgccgagagg cgatcgctac gaatatctgg tccgtgccac tgatcctgat
     2461 ggtgatcccc tcacttatgc gcttgaagtg tcgcctcccg gaatgaccat agaagccgaa
     2521 accggccaga ttcactggat gatcagcccg gaaatgacag ggagtcagcg ggtcagagtc
     2581 gttgccagag atccgcaggg tggttttgcc acgcaagatt ttgatctatc gctgaacaaa
     2641 ccgaccaaat cctaaacatc tcgcttcctc acatctctct tcacatggat ccctcgtgca
     2701 tctgccacca gcgcactgat gagtgcttca cacgttagca tcgtttactc tgtggcgtag
     2761 gcaagcgcag taggaggcaa tttgagggta ttgaccaaca catcgatggc cagacgggga
     2821 tagccagtca gtccccttgc gagcgattcc agtggtaggt atgcgacgtc cggattctgt
     2881 agcgttccct tcgcttcgaa gaccgcggtc gtcaatccct gatgatcttc gccgaatagt
     2941 tgcccgaaga gtggaatctt cccgatcaag tcggaatagg ccgccactgg actcaccgct
     3001 acagcgagat ccagcccatt atccttcacg tcagcggatc ctgcaccagt caccttaatg
     3061 acaggactat ccaacacgat atctttagaa gaaaacacgc cgttgctgcc tacgactcga
     3121 gcagacagtg aatcaaaggg aatcccgcga acgccgaaat ccactggccc gaaaaggaca
     3181 gaggggagat tgagaatttt caggaccttc gtcagcactg ggtcttgttg gagcctcccc
     3241 ttcgtcacct tcacaacgat gccgccagga ccggtactca atgtatgccg cagaggcgag
     3301 gtcgcattga ttcgggcctg cagaataccg tttagagaga gattaccttg caaaagctcg
     3361 ttattgtcct ccgtcgccga gagcaattgc tgagctggta ttccatttac agacatttca
     3421 gccgcaactt ccatctggtc tcgcgcaccg aatcgggcct ctaccaaccc ggacaacact
     3481 ccttcttggg tttgacctcg caaccccttg agcttggcgt tgtgctggtc aaccgtgatt
     3541 tcacctgaaa ccgttttgag aaccaaccgg tcataccgca gttgtttcac aaggaatgtg
     3601 attgccgcct tctttgattg aaaccattcc tgcacatgac tctccttcgt atctggatcg
     3661 accgtcttgc tggacacaaa gtcagagaca ttgagctggg atgattccac catgaaggtc
     3721 gctcgaggat gggttcgcca gtcctcaacc atgccagtca atttgaaatc gctctctccc
     3781 attgtgaaca acaattcatt caccaggagc ccgcctttcg aaagctgtgc tcgtccagaa
     3841 agctcgcgca actgttcgtc gaggcccgga aaagccacaa cagcgcgatg gatgctgacc
     3901 caccccgaag gcacccataa cttccagtcc gtgccttcac cgctaatgag cgcagacgct
     3961 tcgagcatgc cctcgctgat aggcgattcc acaccgatcg cctgcagggc acgcaatgac
     4021 agcggccccg cattcacgtt aacgtgaaat tttggtgtat gacccaggtc cagctgtccg
     4081 gcagctcgta tttcgccatg cggtaccacc aatctcaagt gatcgaccac gacccgcttc
     4141 ccgtcgaaaa gctttccttc ccattcaatg cgccctggaa taccctgctt cttgttaaga
     4201 agagatggca cgcttatctc tgcatcagtg acatccagtg tgccgcggca atggacgagc
     4261 gctgcagtgc ctgacagaaa cattgtcgaa tgggctggtc cgtcaatgtg cacgtcagaa
     4321 gtggccggca agtaggtcgc caagagtttt tgaatagccg tgccatctga cgacatgttc
     4381 accgtgaggg tattgacccg gtttggtgaa gctacgtcga tgttcccctc cgcctcaaaa
     4441 tggatccctt ggacgacacc actgaggtgt ttgataccca tcaagcgaga gtcagctgca
     4501 aacgctccgt tcacttggcc gatcagcatt gctctggcga catgagcccc gatgtcttga
     4561 agatcaaggc ttgcactaat catccgcaga ttgggtctgg gaatgatggg tccgttcaca
     4621 tgaaccccca cgtgaacagt tcctgtggcg tcgctgaccg ttggcaatgc atcgtgggcc
     4681 cccgtgcctt cgctgaaatg ttcgaccaca gccatgacct tttcgaacgg gccctcagca
     4741 agaatgtggg catcgacaat cggtctcacg tccaggtcag agacggagag cttgctactc
     4801 gtgacatgca cgccattcac ctcgccagca aaatccgtta tgtttgcatg ctttgagttc
     4861 aaccagactg tcccagacac gttgcgaatc aatgtgtgtg ttttctccag gcttcctctg
     4921 gcatgtatga ccttggccgc catctgccac tcgtctggta tgcgaagaac gtcgatcctc
     4981 ccacgcaatt caacggattc caactcgaca gtacctgtca gttcatgctc gtcaacagcg
     5041 gcgtgtatgg ttttagggat ccaagaagga ggaatgttgt caaagaatgt cttagaactc
     5101 acaggagatg tcgacagctt gactgcgtat gccggatgat cggtgccggc gccttcaacc
     5161 atgatttggc cacgcatcag taaccatcct agatcagtat tcaggtcgga gacgacgaca
     5221 ttaaatccct tggcatcagg cggaaaaacc aggtgcagac cggcggagat gttgcctcgc
     5281 caagccgcca tttgtgttgg aatcgccgca gactgaccag tgcgtttcaa ccagtacgcg
     5341 atatcccatt ggtgaaaccg caagattccg tcgaactgtc ttgggatagc agtattcgac
     5401 tgaccgtgtt gctgaagggc cagtgtgccg gcaatgtgaa gatcaccgcc atcatctgtt
     5461 atccccgtga gcatcaggtc ggcttgagtc ctagtcggat ggctttggaa cagcacttgg
     5521 acatcgtgaa ttccaatgcc ttcatgctcg agacggttcg cgtccaaaat gcgtagcttc
     5581 ccgtctgtta gcttggattc tgtcaacatc catcgctgag gagaggtact atctcctgtc
     5641 tcggtttgat gtggctcgag taacggtaga tgccactgtc cattggcatc cctccgtatc
     5701 acgacttctg gttcgctggc atggagcgtc agcattggta ctcgatgggt gattgacggc
     5761 caaagaccta atgagagcga agccttggct gctcgcaagc taggctcaaa ggtgagagca
     5821 tctctcacca ataaatcaga cacgtccata cgaactgtag gcaatagact cacatggagc
     5881 gtggctatct caatccccag ttggttcttg agatgctgta cgatcaggtc atgagcgcgt
     5941 gaatggttaa gaaggagact cgccgttgag agagatgcaa tgacaaaagc ggagagaacg
     6001 agcgaccagc ggatgacccc cacacgacgc gatgctgctt gcatggttgt ggtttccata
     6061 atcggcgtga gcccgtcgtt ttgtagagaa tgcgaatgat cagtcagaca agtcttacag
     6121 ccgcctgttg tatgaacacc tccttgggct taaaacgact taaccgcaat tgtttgtgca
     6181 agagccggac ctatcttgtc atggccggct taagtccctt ccgctcaata caatttgctc
     6241 agagttcctt gcttcaaagg acgttgtgtt cctcattatg gacaaatgtt ctcctgcaag
     6301 ggatcgttgg aacatgtggt ccaggtcaga aaagacggac agggcaacgc agtctcttgc
     6361 atcgaacgca cgatgagcct gctctattgt ttcttgtggg tttaggtggg ctgagggagg
     6421 agtgcgcagg tcagattgtt ccgttcagcc acttgtgcga cgacgcgggg agtcgacagg
     6481 gctgaagtcc gatccatttt atatgcgctc gacgatgatc gccaatgcct cgccaccgcc
     6541 gatacacaag ctggccaatc ctcgccgggc accacgggcc tccattgcgt acagcaacgt
     6601 cgtaatgatg cgggcaccgg tagccccgat gggatgaccc agcgcgatgg ccccgccgtg
     6661 aacgttgact ttctccggat caagccccag ttcacggttg atagcgagag agacggcaga
     6721 aaaggcctcg ttgatttcaa aaagatcgat gtcgccgagc gaaaggctgg ttttctcgag
     6781 cacgagtttg atcgcctcca ccggtgcaat ggacaaccac tccggtgcca gagccgctcc
     6841 cgcgtatcct acgatccgcg ccatcggaac gagcttccgt cgtacggcct cctcttgcgc
     6901 catcaccacc agcgctgctg ccccatcatt acaggaaggg gagttgccca cggtgagcag
     6961 gccgttttcc tgaaacaccg gcttgaactc gcgcagcttc ttgagatcga ttcgtttggg
     7021 ctcttcatca tcaatgatgg tcagggacgc gcccttccgt tgcggcacgt cgaccggcgc
     7081 gatctcaggc ttgaaagcac cggcgtcaat cgcgtattgc gcgcgttggt agctttccaa
     7141 ggcataatca tccagttctt ttctggtcag gcggtacttc gcggcgcaca gctcgccggc
     7201 attgcccata tgaaaattat tgtagacatc ccacagaccg tctttgatca cgctgtccac
     7261 catctccgca tgacccaacc ggtatccctg ccgtgccttc tctaagagat acggtgcccg
     7321 ggtcatgttt tccatcccgc cagccacgac gatgtttgcc tcgcccagtg cgatggtctg
     7381 ggaggccatc atcaccgtct gaatgctgga tccacacacc ttgttaacgg tcgtggcgcc
     7441 aatcgaatgg ggaatcccag ccccgattga ggcttgtctg gcgggcgcct gacccaggcc
     7501 tgcactgagg acacaaccca tcagcacatc atcgacgtgt tcaggcaaca cgtgaacgcg
     7561 cttgagggcc tccacaatcg cgatactgcc cagcttggtt gcaggaacgg aactgaacac
     7621 accgttgaag ctgcccatcg gcgttcgcac ggcgctgacg atgaccgcct gtctcttctc
     7681 agtctccagg cgttctgact tgagtgtgat cgacattcga ctgtcctgcc gaaagatcgg
     7741 aagaaagatc ggtagggttg gatggattca tgttacgagc cgctgtgtca tttctcaata
     7801 caggaatgat tccttcttca agccagtcat attgaccgct catgagcatc ttcgctaggc
     7861 gatacacgac gctatctttc ttgtgccgcc ggattcttcc ggccagagag gaaggtggac
     7921 acagccgatc cgccatgtcc tgacaaaaat aatcgacgag gtcttgaatg ccgggcttcg
     7981 tccgcatctc cggctgattg gcataccaga gggtggcggc catgggaaag agcaagaggc
     8041 tgtcattcac cagatgttgc aacagaagct gcttattatg gagacgttgc tgatgacgga
     8101 gcgtcgcctt gagtgtcgac cgggtaatac ggcgtgcagc ggcctcgata aaacgaaccc
     8161 accggctatt ctcgtctccg aatggagcgg gtgtaggacg gagaaaggga aggcacgagc
     8221 gcagagacat gtgcgcataa tagagcggag cggcgagctt gtcggtccat gttccttgga
     8281 gaaaggcgag gccacggtcg atataaggcg agagcgcttc gcgcgccatc cagacgcgca
     8341 gaatctcgct cgttccttcc cagacgagat tgatgcgagc gtcgcgcagc atgcgcatga
     8401 ccgggagaga aggttcgccg ctcttccgct gggcctcagc ggtcatgaat cctcgtccgc
     8461 cgcgaacttg aaacagttca ttgaccgctt cccaatacca ctcgctcccg atgattttgg
     8521 cggctgccga ctccagtcga agatcgcctt tcttgttcgc ccaggctccg cagaaggcca
     8581 tcacggcttc cagcgccagg gtatacgccg cggttcgaca gagtttctct cctatcaaat
     8641 tgtgttcgcc gatcggcctg ttccattgaa cacggctctg tccccaccag cgcatgaggg
     8701 acaggcattg tttgaggccg cccaggcagg ccgcgggcaa cgtcaaccgt cccaccgtga
     8761 gtgtggcaag ggcgattctc agaccgtctt cttcccctcc gagccggttt tcaacgggga
     8821 cgtagacgtc acgaaacgtc gggataccat tgtaaatcgc tctgactcct tcgaaccgga
     8881 ggcgggtgac agaacatccc ggccattgtg tttccacgat aaatgcgcca tagcgtttct
     8941 gggatcgtgg atcgttcacc tcctcggggc gatccacaat ccgcgcaatg acgaccagca
     9001 tggatgacaa aaactcgcca tcccgtttgg cggagtttgt aatgaaaaat ttttctccag
     9061 tgagccgata gccgacggtc ttcccctgct ccagaatacg caccgcatag gtttccacct
     9121 tcgaaatgtc gcagcccacg ttgcgttcgg tcagcgcaaa accggaaatc tctccccgcg
     9181 ccaatcgtgg aagatactta gctttctgct tgtccgttcc gaagaggcgc aagggctcgg
     9241 gaaccccgat tgattgggcg gccgagagga gcaccgtcaa cgatgcgtcg acgcttccac
     9301 aaagggtggc gactttttga tattcggcct gggtgcattc cagcccccca tattggcggg
     9361 gaatcttgat gccaaaggcg ccgagctggg cgaactcctt caagagcggt tcgggcaatt
     9421 cgccttcgct gtcaacgcgt tcggggtcca ggggaagcag ggtggtcctg aatcggtgaa
     9481 gaaaatcaag ggccagcgaa ctcataggag gaatttcgat ggaggtaaag agcttccatc
     9541 gaacgtcgga ttcgaagagt ccagcgagaa aaccggtata ggaggcccgg tcgcgggcgg
     9601 tctcagcgat cgccacagaa ccctgaaata tatccggttt actcatgtgc agcggggtcc
     9661 ttttgaggac ttcgagacaa gagctgcccg tatccttctt agtttaccac tgtttcctgc
     9721 cgactgtgtg taggcaagag ggttattcgg tccctctaat gtaggaccct taagaaaacc
     9781 atggaaaacg ccaatcccgt ggtacgatat gggtagtcgg gcctcatggc atcggcagtg
     9841 atttcttctt ccgaatttgt ggatggagat tccacgatgc gcaacgtggt cgtcgtcgac
     9901 gggttccgaa ccccgctgtg taaggaaggc acagactttc gcgagacgga tgcggaggta
     9961 ctgggtgcct gggtcgtgcg cgaaatcatc acccgtctcc accgctggaa tctcccgctc
    10021 accaccatcg attgcgtgct cggcagcaat gtcgcaacgc ccatgcatgc ggtgaatccg
    10081 acccgcgtcg cagcggtgac cggaggtctt cccgctacga ttcctgccga tacggtcgcc
    10141 gggaaaaatt gcggatccgg tgtgacggca ctctactact cgggcctgcg tatcagaagc
    10201 ggggatgccg acacggtgct cgcgattggt atggaggcga tgagtcgtat tccactcgtg
    10261 tatgaccgga ctgtcgccga cctgcttctt caatatggga aagcgagaac gcttcgggaa
    10321 cgaaccgccg gtgtcctggc tctgatccca aagttgctca atctgaagcg ctatccgcca
    10381 cgagtcggat tgatctccgg tctcacggat ccgatgtgtg acctcattat gggtctcacg
    10441 gcggaaaaca tttcaaaaga tcccgccctt ggcattactc gggaagacca ggatgcattt
    10501 gcggtccgtt cgcataggaa tgccgcacgc gcatggaaga atggactgct cgccgacgag
    10561 gtcgtcccca tgtatgtccc ggagcgaagc gcctatgtct cgcgggacaa tggcatccgt
    10621 gaggatgcga gtgcgcaaac gtttcgtgat gtgaagccgg tattcgacag gcaccacggc
    10681 tcggtcacgc cggccaacgc ttcgcaaatc acggatgcgg ccgccgccat gcttctgatg
    10741 gaggaaagta aggccaaatc gctcggcttg ccgattcttg gatacctgaa agagtatgcc
    10801 gattttggct atgaaccttc ctgtatggga ttggcccccg tgggagccat cgccaccgta
    10861 ttgtcccgcg ctcggttaac cctcaaggat tttgctgtat gtgagatcaa cgaagctttt
    10921 gcatccgtgg tgttggggat catgcgcatt cttgactcgt ctgctcttat ggaacgcaag
    10981 ttcggccgat atggatggac cgagcggttg ggcgaaatcc cgtatgacga tttgaatccc
    11041 cacggcggtg cgatcgcact cggccatccg gtgggagtgt cgggtttgcg cctggcgatg
    11101 accgctctgt atgaactcaa acgtcgaaat gctgagcgtg ccttgatcag catgtgcgtc
    11161 ggaggcggac agggtgtggc catgatcttg gagaggaagt aggtatgtcg cacgatgggt
    11221 cccaatcatt ctccctgccg gccactctca aacacttcaa gacggcaatt cttgacgatg
    11281 gaatgttgct ggtcggattc gactaccagg ggaaaagcgt caatgtgctg aacgtcgaga
    11341 gcatgtccga gtggcagcac attgtgcagg cagccgagcg atcgaccacg atcaccggcg
    11401 tgctgttgat gagtctgaaa gaagggaact tctgtgccgg agccgatctg gaacagatgc
    11461 acgaagctca gcggcaggga tccttccgct ccatggaaca actcgtcgag atcgcccatc
    11521 aaatcttcga tgccatggac aggtcggcca aaccatttgt cgttgcggta gagggctcct
    11581 gtctgggcgg tggactcgaa ttggcattgg catgtcatag acgcattgcc agcactcatc
    11641 ccaaaacttg cttcgccctg ccggaagtca aactgggtat acttccgggg ttcggcggaa
    11701 ctcaacgtct tccgcgtttg atcggtttgc cggcagcgtt ggacttgatt acggcggggc
    11761 gaatggtgtt cccccgacag gcgctacgga tgggtcttat tgatggaatg gtcagctccg
    11821 ttccctctcg catgagaaca ctggaggcca tccagacaga aaccctgatt caggcggcca
    11881 tttcgcaggc ccgtttactc cgctccgctc ccgcccgcca gcggcctttg aggctaagcc
    11941 agagactcct gggcggtcct ctcgttcgtg tgttgctctg cgggtatgcg agacatcaag
    12001 tgaagaagcg cgttcggcac ttctatccgg ctccgcttcg agccgtcgac gccatgcaac
    12061 gcgggatggg aatgagtgtt ctgaaaggct cgctgatcgc cgagaagcca ctcctcatgg
    12121 agctcatggc cagcccggtt tcccggcatc tggtcagcct gtttctagcc ggtgaagatg
    12181 tgaaacggaa ggcggccccg gttcctgatg cggtgactcg cgtgggagtg ctcggggcag
    12241 gattgatggg ctcgcaaatc gcggggcaac tcgccgaaaa gggatatgcg gtcgtgttac
    12301 gcgacgtcgc tccgagtatc cttgccgcgg cgatggggcg aattctaaaa gctcaaacga
    12361 accaggtgcg caagaaaatc atactgccgt ctgagctgcg ctatcgcttg ctgcgaatcg
    12421 ctccgacgac aaccatcaag gacgtgagtc cagcgcctct ggtgatcgag gcagtatcgg
    12481 agaagcttga cgtgaagcga tccgtgctcg ctgagttcga atcagaggca gggcccgatg
    12541 cgatatttgc cacgaacacg tcctcgtaca cattgacaga cattgctctg aaggcggtcc
    12601 atcctcaacg gtgtgtaggg cttcactttt tcaaccctgt cgccaagatg cagttggtcg
    12661 aagttgtgcg ggctcccttt acatcagacc aagccctcgc ccaggcctgt ggtatcgcca
    12721 agcgcctggg caagttcccg ctcgtcgtac gcgatgggcc tggatttctc gtcaacagga
    12781 tcctgtcacg atatctggcg gaggctgtca tcttagtcgg ggagggcgtc ccgatccggc
    12841 gcatcgacac catagccaag gactttggca tggcagtaga cagcggacac cccatggggc
    12901 ctctggagct cattgatttg atcggtctac cagtcgcgat gcatgtgctg gcgtcgctgg
    12961 cggtattggg atcacgaatc gaatcacgcg acgccctgct gcggaacttg cttgcagaca
    13021 ccaggacacc cgtgccgttt tggaagtccg gagtggaaaa tagacaagcg ctggacgtga
    13081 tcgccgacta tcggcgtacc tatccttcag aagcccggcc ggtttccgac gaaatccttc
    13141 accagcgtct cttccttccc atggtcgacg aagcggtgcg ctgtctgcat gacggtatcg
    13201 tggaacaggc ctgggaggtg gattttgctc tgacctatgg gatcggattc cctgcgtttc
    13261 gcggaggcct gctcacgtgg gcgcggcaaa ccatgacgcc tgagcagatt gccaggaaat
    13321 tggacgaact gagtcacaag tacgggaagc gctttgagcc ctttccggga ctttcgcacg
    13381 gagggtggta gcgacaacta tttccactga acgaacggag cgcgtcatgc atgaacaacc
    13441 gggcgtccac tatcccgaag gggttcctcg agagctccct ctctgttcag agactctcgt
    13501 ggacatgctc gcacgcagcg ctcgacgatt ttcacggcga ccggcgctct attttttcgg
    13561 taagaccgtc tcgtaccgtg aatgccacga tctggtggag cgattcgcaa ccggtcttgc
    13621 cacccgggga attcggaagg gtgaccgcgt cgcgctgtgt cttccaaact ccccccaagc
    13681 cgtcatcgcc tacttcggta tcttaagggc tgggggcatc gtcgtcgcct gtaatccgac
    13741 atacacggag cacgagcttt cccatcagct ccgagatgcc ggcgcgcggg ccattgtggt
    13801 ctttgaccag atgtaccaga agattgagca tcttaatctc gagctgatca tcgtcacgcg
    13861 gattactgat ttcatgacga cgatcgcccg gtacatgtac caccatcgaa acggccatcg
    13921 gtctccagag attccgattg gaggcacaac cctctggttc catgacgttc ttcatcaggc
    13981 gattcccgtc gagccagaaa acctcccggt cgtggtgcct gacgatatcg ccgtcctgca
    14041 atacacgggc gggattaccg gtgtatcgaa gggagcggag ctccgtcatc gcaatctgac
    14101 ggccaacatc caacagctga tcagctggtt ttctcccgcc gagcgcgtgg agagctttct
    14161 tgctgcgctg ccgctcttcc atgtgttcgg gatgaccgtc acacaaaaca tctgcctggc
    14221 ggttggtgga tgtatggtgc tggtccctga tcctcggaat atgcccgtgc tcctcacgct
    14281 gcttcgaaaa acacagccga ccgtgatcac gttggtgccg gcctttgtac ggaagttgct
    14341 tgaacactgc gatgcaaagg atttcaccgc cacccgcttt gtgatttgcg gaggcgcctc
    14401 gctttcctat gagctcttaa aaaagttcaa ggagcggacc ggtcatatga ttgtcgaggg
    14461 gtatgggctc agcgaagcct ctcctgtcac gcactgcaac cttccacgcg gtctctcggt
    14521 caagcgatcc atcgggctcc ccatcgccaa tacagaatcg aaaattgtgg acgcggccgg
    14581 tcaggaggtg ccggatgggg aggtgggtga attgtgggtg cgaggtccac aagtgatgga
    14641 acgatattgg aacaatcctg tcgaaacggc gaacgtggtg aaaccgggcg gttggctcgc
    14701 gaccggcgac atggcgcgaa tcgatcaaga cgggttctac tatatcgtgg acagaaaaaa
    14761 ggacatgatt ctctcaacct cgggcttcaa tgtgtatcca tcagagattg agcacgtcct
    14821 tctcctccat ccgctcgtgc acgaagccgc cgtaatcggc gtgcaggtca cagacggatg
    14881 cgaagcaatc aaagcgttta tcgtcttgaa ggttgaaacg gtcagtgtgg atgaactgct
    14941 cgcacactgc cggcactatc tggcgtcgta caaaatgcca aagcacatag agttccgcac
    15001 ggaactgcct caatcgattc tcgggaagat gctgcgtcgg gtgttgcggg tggaagaagg
    15061 cggacgactg cggcttaacg gtgagacacc gcaccggata aatggccaga acctcgcggg
    15121 ccaaaacacc tctggatgca aggcaggtca gtaacatgtc gagccagagc caaggcggcg
    15181 gagagtccag gcgattcaat cccgataaga ttgacaagcg gtggattctc tctatctgcg
    15241 aagacgatga aatcgctctt gcgaccctga ccagtcatga cacaaggcct gattccggaa
    15301 taggcccagc gaagatcgcg ttcgttcaag gctggaagaa agttctgcag ggtttcaagg
    15361 aacatgtccg gcggcgtctt ccgcgacgta tagtccgttt tatccaggac ggcgaactcg
    15421 ttcggtccca ggaacagctg cccgttgggg cggggactga agtggatgcc ctttccggtc
    15481 gcttgacgag gcaaagcggg atagatcaga cgcccaatca ggcctctttt ttccggggag
    15541 atcacttcgt agtactctcc ccgaagggga cggatcgcgt acttggttgc gcgtaaggcg
    15601 agggacgcga tgtcgtcggc gtacagtccg gcggcattaa tgaggcaggt cgctcggatt
    15661 ttcgtacggt cggtcgtgac gaggtacgac gtggaatctg catcaatctc aacgacccgg
    15721 ttgttgaaag cgaacgcaga gccggtgtct tccgcatcgg ccctcagggc ttgcacgaag
    15781 gccagagcat ctatgacgca gacgctcgga atgaggaggc cggcgcaacc gcataaattg
    15841 ggctccaatg ccttgagcct atgtggcgtg aggaatgaga ctcggatgtt ggatttccag
    15901 gcgttggccc aaagccgtcg tagcgaagac acgtcacgcc ataggccacg acgtacatcc
    15961 tgccatgaga acgcaatgac cattcccgat ttcagcaagg gaatgccgcg ttggaccgcg
    16021 tacgagaccg ccaatgcgct tccctctcga gccagccgag ctttcagcga attgggattt
    16081 tcgtggattc cgctatggag gacgccactg ttgcggctgc tggtctcttg gcctgcttgc
    16141 gccccttgtt cgaatatcac gacacggagc cgagggaact tgtgcccaag ctcccttgcg
    16201 atggcgcacc cgacaactcc agcacccacg atacagacgt cgtatgtttc catctggcgg
    16261 ggtcctttaa gacaaagtgc aggcccagtc aacaaaccgg agcatgtgca tgtcggaact
    16321 cagtagcaag tatgggaaga ttttgtttgt gttttgcgga ggcggctgta aggccatcat
    16381 ccaagccgtg gcggcctcag aattggtgaa agccggtttc actccttccc acatcatcag
    16441 ttcctccgcc ggcagttgta atgccttggg atttgttgaa aatcccgggc ctgtcggggc
    16501 agagaacacg atccgcatct gggaagacta tatcacttca ccggaagcga tctatgaagt
    16561 tcacccattc tttcgagaga aattaacgcg tctgttggga gtcgtgccgc gagccaccca
    16621 agattgggga ccatccggat ccattcttca cgatctcagg cgggcgatga agtttgtacc
    16681 ccttattctc tctttttgca cccgcatgcc gctccgagtg gcggggcgca ccgtgagttt
    16741 catttttcgc ctgatggaca cattcgaatc gcagcataaa tcgttccgac gactcgtcca
    16801 gtgcccggaa attcaggaag cgtttgacga ggtagataag ttttttgaat tcaagaggat
    16861 gaaggccttt ctcgacccgt tcccgcttct ggccaggctc ggcgagcaaa ttgatctaaa
    16921 gaaagtgctg accagttcca cggtgtggca catcctcacc gaacggtatg aagatggagc
    16981 aacggtcatt ttttcgaata aggatcccga tgtcgcgatg gacggcaatg atgacgcgcg
    17041 aagcgtcaaa agcaagtacg atctctttta taaacgcatc cgtgcgtcca tggctctcta
    17101 tccgctgttc gagatggtgg acatgggggg gtatcgatac cttgatgccg acctcgccaa
    17161 ccctctcccg gtcgaagagg cgttcagtgt tggatgtgat acggtgtttt tctttcttaa
    17221 cgttccgaag aagagcgttc gagtcgaccc acatccattg cgagaccttc ttgagttgaa
    17281 cgatctcctg cgattgaacg agcgctttat tcaccaccgt gtcatggagg cgcaggtgaa
    17341 agcgaagaca ttgggggtga atctgtttgt cgtccgtccg gatgagatcc cttccggcct
    17401 ggggttgctg gagattgaca acaaggtcat tcaatccgtg aagatacacg aacgaaaccg
    17461 catgaaggag tatctctgca acctggatac gcacaacctt cgatttgttc cactctctga
    17521 tgagtagtcc tcgcaggcct tcctcggtcc cggctccaag tgatcccttg cgtcgtatgc
    17581 tcaaaatcgg gcatcgggga gctgcgggac atgcgccgga aaacaccttg gcagcgattc
    17641 gaaaaggcat agctcttggt gtggacttcg tggaaattga cgtgcgatgc acggcggacg
    17701 gcgccctcgt ggtgcttcat gattcgaccg tcaatcgtac gaccaacggg aaagggccga
    17761 tcgatcgctt atcgttccgg gatgtcaagg ctctcgatgc agggaatggc gaatccatcc
    17821 ccaccctcga agaagccctc cgcgtgcttg caggacgaac tggtctgatg gtggaactga
    17881 aagttaaggg aatatcccga aagactgtag aggtcgtaca gacctgccga ttcaaagagc
    17941 ccgtcattta tgcgtcattc ctgcacgaag agctggggat tatacgatcg atcgacgcac
    18001 aggcctctat catgccgctc ttcggcaggt tgcctcgggc ccctgtcgtc cgcgcgatga
    18061 aatacagccc ctcacacgtc ggtctccgtc acgacaccat ctcccgacgc ctcgtcgagg
    18121 cgttccacga ccaggacatg ctggtatgtg tgtatacagc ggataggaag cgtgacatcg
    18181 agcgtgcatg ctccgccggt gttgacggga tcatatccaa tgttcctgat cgggtggata
    18241 acgttcctgt ttgaatttgg cctcatcagc tattcgcacc tctgcaagct ttgtgcacca
    18301 gccgtaaacc tgaaggtggt caatgaggct ccttccatag tgtggggtgc caatttccag
    18361 ggaaaaccct aattaataaa atgggagcgg gtctttatgc taatcccagg tgcgggagct
    18421 gagtggcatc agttcctccg agatgactca ttctggagaa aggagatcga tatgcttcgc
    18481 tggtcagcag tctttttggt gatcgcgatc attgcggcgg cgcttgggta tggcggcatt
    18541 gctgccgatg ctgctgagat cgcaaaggtg ctgttcttta ttttcttggg gatcttcgtc
    18601 gtgacactca tcctcggatt gatagcgggc cagaaattat tcggtcaacg gagataacaa
    18661 cacgaggaac ggttctgtag attcgcggct gacgatcacg gcaggagcca ccttcgcctt
    18721 gtggactggc tgtatcggct cacttccccg agtgagccct catgtgtaat gttgtccctc
    18781 cttgtccagg ataccgtcat ctctcaggtc ttgctctcca tcgagatcga ctgagtgcag
    18841 cgacacgtcc gataggaggc aatggagggg atcagcatta cctggccgat ggttcgactg
    18901 ccgctgcctc tgggtagcgt aatcgcacgg tcggcttctc tgacagatat ttccgagata
    18961 gttcgctcaa gtagtattcg agaaggtcac gctcctcgtc ggtcaactgc tggtcgaggg
    19021 aaagtaaatg ctcacaggca cgtgtgtagg cttgtatttc tcggcggaga ttcatagtgc
    19081 accagccttt caaaacgaag tggatgttgt gaaagatggc ggtgcatgaa gggatgaaaa
    19141 atatgcatta gtattgtcca acgggatatt ctggtcaagg gttttgcccg taggatcttg
    19201 ctgacagctg tgtcagtaaa aaagtacaac aaaccattga aaaatcagtt attatgaaca
    19261 agagtggcga gatggaaccc acggtcttac acaattttta tagggctaca caaagtcttc
    19321 gcgaggcgat ggaatccgat ctgcctttga atgatctcga acgcatttcc ctggaaaatt
    19381 atattgccct gctccaaatg acctacatcg agtggaaaag aaggaactgt caaccgcccg
    19441 cctatcgaaa agcggcttaa acgttcttcc tgtcatttcg gtcgattccc tctgtttttc
    19501 agcctcccca agagggctct ttcagttaac ttctcgactt aatacgcggc agtacgtaca
    19561 gaaattgtac gtagaagaag aatttcgttc atttttgtct cagcatcgct cctaagtagt
    19621 tgaaactacg atgatcggtg cgggggccaa aaattgctat gagacgtcat agcttttaac
    19681 ctggcccgga ggatttatgg attgcccaaa atgcaatggg ttaatggatt tagagcggtt
    19741 ctcggatttt tttctcgtct tctatgcctg gaagtgcatt aactgcggcg ccgtgatcga
    19801 caaaaccatt gcgatgaatc gaagaaaaag cttggcggcc aaacaggaac ttaccaaggt
    19861 ctgcgtggaa tccacatagt tgaggggtcg ctgcctggct cttgagaaac aattcaggca
    19921 ggaggttctg catgcggtct cacaacgaca ggcctttcag gaatgtcgag aaggttgccg
    19981 tggccgcgga atttgtgcac gcggccgaac gcctcattct ccatgatgtc aagccagagt
    20041 ttcttccaga gcgtgaacgt gcagctcttc agtactacct tgattgcctt tcgaagaagt
    20101 tcagcgaagg ccacgtaaag gagccaagct gagcacgaac agcatttcac cctttccgaa
    20161 cacctgcccg tcgtaagact cttccatcaa tccttatcct tctgcccgca caaaagtacg
    20221 cttgcgactc gccacctgtc gatcaataga ggtgctgtcg ctattgcaaa gattccagcg
    20281 gatcgagaga acatccgatt ctgccggtac cgataggaac agccggatgt aaggatctaa
    20341 tggaaggcaa gggtatcgcc gagattcatg ctctcggcaa tactcctgag aggcgtatcc
    20401 gcactatgta tactgggcca tgaccataaa gcgtccggat ctatgcgacc cgtttgcttg
    20461 tccggtgatc gttcgaatcg cccggattgg tgacagggtg cgagccaccc tttatcggac
    20521 gggctcggcg aaactcggcc acggcctttg ggcctacaaa gaaaacgccg atgatacagg
    20581 ccagcaaggc tgcaattttg agcatgagaa acacccgctt ccagatagtg cgataagaca
    20641 gtttacaact gcggcaaaga gtcgtcgcct aggaacccct ctggccatgc tgaccgaagg
    20701 gcgcatcttt gcgctgcttg aggagatctc tctattcaat cgcccgtctc gtcgtgtgga
    20761 tgactgacgg gtttcgattg aggagatcct ttttgacgga gaaaaatgga taaaataatt
    20821 aagaccgcaa ctgaaagaaa ctcactctgc caattctgta atgattcgaa ccaaaactgt
    20881 gagaccatga tgtattcccc cagcgtcacg gcatgtccgc cgtggagggc ttgctcctca
    20941 ttgaacacgt gtcgaccagc gacagcatga agcaggaaag aaatgataaa gagtaagaaa
    21001 agcgcgagcg taagagaata ctcataaatt ttgcgtatcc at
//
LOCUS       DASSCQ010000114         4936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_7746, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000114 DASSCQ010000000
VERSION     DASSCQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7746"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1856)
                     /locus_tag="VFH05_08030"
     CDS             complement(18..1856)
                     /locus_tag="VFH05_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6678201.1"
                     /translation="MWAFFARVLLSWLVGLSAAGFLHSASAAEGEGSERSDVVETPEV
                     FISATKTEVPVKQVTSAVETITGEQMQQRKIKTVVEALRLAQGLAVFQSGGPGTLAEV
                     RMRGGTPEQTLVLIDGAIVNSATLGSYNFANLTTDNIERIEILRGSQGMMWGSDAMGG
                     VVNIITKRGRETPAVSSFVEYGSFATIREGASAVGKKGPVDVAATLSRWDTSNFSAIN
                     YRRGAAERDGFHNWQGSAKLGVDLPKDGRLEFNFRWMDGITNLDNTLPDPSDTLGAKT
                     QSKQYVYTGSYMQPITNWWSQRLTLARATESLTTSSGTLERNLFTGVESPPFPLNSEI
                     NTTSNRLEWQHDIQIAKPLSVTAGYQFREQLGENLDTLTGTTTLPTKIISSNAGFAEA
                     KLNLWDRLFGTAGIRQDQYNVFGSATTYRATAGYLHTETGTKLRGSYGTGFRAPTINQ
                     LFFPEFGNVNLQPEKSKGLDVALDQSLFNDRVQLSVGYFWTRYQNLILAVFDPAGACS
                     FTPFGFCAQNIGQARAEGVEASAKVKLIRDQPWIKSLDLQFQYTHTSTENLTDNAGTR
                     LPRWPLNQWSAILSYQPIEALRANLEGRFVGQRFNDPSNDDSLPHF"
     regulatory      complement(1903..2066)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2174..3004)
                     /locus_tag="VFH05_08035"
     CDS             complement(2174..3004)
                     /locus_tag="VFH05_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6678202.1"
                     /translation="MMPQQNAYEVRSVFFRYGSAHVGTTWAVQDVNLQVKAGEILGIV
                     GPNGSGKTSLLKLLAKIVRPHTGEITLFGKSLESATHREVARQVAFVPQDNQPAFSFS
                     VAETVLMGRFPHRHSSFWGSAFGWDSQEDCAVAQQAMRTMDVAHLANRSVTDLSGGER
                     QRAVIARALTQTPKVLLLDEPTAFLDLQHQLEICSILRALNEERGLTIVMVSHDLNLA
                     SQHCDRILMLKDGVVAKMGSPVDVMLPEILQAVYGCHVLVDSHPESGLPRITLPRQLH
                     "
     gene            complement(3039..4046)
                     /locus_tag="VFH05_08040"
     CDS             complement(3039..4046)
                     /locus_tag="VFH05_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET6678203.1"
                     /translation="MATLSTAAIVLTLVCLQMGTQYVSLAEVGRIIGRAFGVGETGAN
                     GSSITDTIVLQLRMPRVLLGFLVGSSLASVGVILQALLRNPLADPYILGVSSGAALGV
                     SLAVLFGLGGMAWLIPTLPIYGFFGGLLALLVLYRMSATYDRLPVHSVLLAGVILNAI
                     FAALIMFITSIMEPNRAFGMMAWLMGSLTATADQTLLALSIYLALCLAWLFKQVNVLN
                     ILTLGEEPARSLGVDTERAKRGILMTAALVTGAVVSVSGMIGFIGMVVPHAVRLVLGA
                     DHRLLLPASALVGGMVLMTADTFARSVFAPSELPVGIMTALAGGPFFIYLLVWRKDRL
                     A"
     gene            complement(4175..>4936)
                     /locus_tag="VFH05_08045"
     CDS             complement(4175..>4936)
                     /locus_tag="VFH05_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HET6678204.1"
                     /translation="TEMLFAIGLDEQIVGVTNFCNYPPAAAGKPKVGYTQPNLESLLA
                     LSPDMVAAPGELLRADVLAKLEALKVPTFIFEAKSLEHVLAHIHTLGRIFERSSAADA
                     LTRHMRERIAEVSRRVQTVERKRVLYVLSSHPLITVGPNSYIHQMIELAGGINVAAGT
                     SGAYPRLSMETVLEKDPEILVFPVGSTESVPEHEQQTWKRWTGLSAVQHHRLHVVSSD
                     ALNRPGPRVVDGLEQLAQVIHPEAFSTDHPTSSRP"
BASE COUNT         1098 a         1451 c         1348 g         1039 t
ORIGIN      
        1 aacgaggcat tccacactta gaaatgcggg agtgagtcgt cattgctggg gtcgttgaac
       61 cgctgtccca cgaatcgccc ttccagattc gcacgcagcg cttcgatagg ctggtaactc
      121 aaaatggcag accattgatt caacggccag cgagggagtc gcgtccctgc attgtcggtt
      181 aagttttccg tgctcgtatg ggtgtattga aattgaagat ccaggctctt gatccagggc
      241 tgatcacgaa tcagtttgac cttggcactt gcctctacac cttcggctct tgcctgcccg
      301 atgttttggg cgcaaaaacc gaacggcgta aagctgcagg ctcccgcagg gtcaaacacc
      361 gccaggatga gattttgata ccgcgtccaa aagtatccta cactgagctg cacccggtcg
      421 ttaaacagcg attgatcgag cgccacatcc aatcccttgc tcttttccgg ctgtaaattc
      481 acgtttccga attccggaaa gaacagctga ttgatcgtgg gagcgcgaaa accagtcccg
      541 taacttccac gaagctttgt gccggtctct gtgtgaagat agccggctgt cgcacgataa
      601 gtcgtggcgc tgccgaaaac attatactga tcctgtctaa tacccgccgt tccgaataat
      661 cggtcccaca gattgagttt ggcttccgca aaccctgcat tactgctgat aattttcgtt
      721 ggcaaggtgg tcgtaccggt gagcgtgtca aggttttccc ccaattgctc gcggaactga
      781 tagccggccg tgaccgataa cggcttcgca atctgtatgt cgtgctgcca ttcaagccgg
      841 ttactcgtcg tgttgatttc ggagttgagc ggaaatggag gcgattccac tcccgtgaac
      901 aggttgcgct ccagcgtgcc ggaactcgtt gtcagacttt ctgttgcgcg cgccaaggtc
      961 agtctttgcg accaccaatt cgtaatcggc tgcatatagc tgccggtata cacgtactgc
     1021 ttgctctgcg tctttgctcc cagcgtatcg gaaggatctg gcagagtatt gtctaaattc
     1081 gtgatcccgt ccatccatcg aaaattgaac tcgagtcgtc cgtccttcgg cagatcgacg
     1141 cccaacttgg ccgatccctg ccagttgtga aaaccgtcgc gctctgcggc gccgcgccga
     1201 taattgatgg ccgaaaaatt ggacgtatcc cagcggctca gcgtcgccgc cacgtccacg
     1261 ggtcctttct ttccgacggc gctagcgcct tcacggatcg tcgcgaacga accgtattcc
     1321 acaaaggagg agacggcggg tgtttcgcgt ccacgcttcg tgatgatatt caccacgccg
     1381 cccatcgcat ccgatcccca catcatgccc tgactgcctc gaaggatttc aatcctctcg
     1441 atgttgtcgg tagtcaggtt ggcaaagttg taagagccga gagtggcgct attgacaatc
     1501 gccccgtcga ttaagacgag cgtctgttcc ggtgtgccgc cccgcatgcg cacctccgcc
     1561 agcgtaccag gaccgccgct ttgaaagacg gccagtccct gtgccaaacg aagcgcctca
     1621 accacagtct tgatcttccg ttgctgcatt tgctcgccag taatggtctc cacagcgctc
     1681 gtgacttgct taacgggaac ttccgtcttg gtggcgctga taaatacttc gggagtctca
     1741 acgacatcag atcgttcgct cccttcgcct tccgctgcgg aagcggagtg gaggaaacca
     1801 gctgcggaga gaccaaccaa ccaactgagc aatacacgtg cgaaaaacgc ccacatgata
     1861 gcctcccttt gactcgaagg gtgtgacgac tttccgtcag ttgggtctcc tgactcgcgg
     1921 atcgacgcgt tcttccgcct tcccagcggg tgcgaggatc gtcgcgtttc cacgcgcgat
     1981 agtctcgccc caaaccagtg gcctctctgg aagaacgctc cccgcttaca gtggcggcac
     2041 cgtgatggct ttgcaccatc ttccccggcg ctgacggctt gtgttggccc tctctctcga
     2101 agttattttc gtgccctcat cgtgtatgcg gataggccgg gcacgaacca cacccgcata
     2161 gtgaacattc agctcagtgc aattgtctcg gcaacgtgat gcgaggaagc cccgactcag
     2221 gatgtgagtc gaccaatacg tggcacccat acactgcctg caagatttcc gggagcatca
     2281 cgtccaccgg actgcccatc ttggccacga cgccgtcttt cagcatgaga atccgatcgc
     2341 agtgctggct ggccaggttc aagtcatgcg aaaccatgac gatggtcagc ccgcgctctt
     2401 cattgagcgc acgcaagatc gaacagatct cgagttgatg ctggagatcg aggaaggcgg
     2461 tcggctcatc gaggagcagc actttcggtg tttgagtcag cgcgcgggca atgacggccc
     2521 gctgacgttc tccgccggaa agatccgtga ccgaacgatt tgcgagatgt gccacatcca
     2581 ttgtcctcat cgcctgttgc gccacggcgc aatcctcctg gctgtcccag ccaaacgcag
     2641 agccccaaaa cgatgaatgc cggtggggaa atcgtcccat cagaaccgtt tccgccacgc
     2701 tgaaagaaaa agccggctga ttgtcttgag gaacaaacgc gacctgccgt gccacctccc
     2761 gatgcgtggc cgactcgaga gatttgccga ataacgtgat ctcgccagtg tgcggcctca
     2821 cgattttcgc cagaagtttg agcaacgacg tcttcccgga tccgttcgga cccacgatgc
     2881 ccaaaatctc cccggccttc acctgcaggt tcacatcctg aacagcccag gtggttccaa
     2941 catgggcaga accgtaacgg aaaaacactg accggacttc gtaggcattc tgctgcggca
     3001 tcatgaacac tcccatcgga tttctatcgg caatgaggtc aagccagccg gtcttttctc
     3061 cacacaagga gatagataaa gaatggacct ccggccaatg cggtcatgat tccaacgggc
     3121 agttctgaag gagcaaaaac acttctggcg aacgtatcgg cagtcatgag caccatgcct
     3181 cccacaaggg cggacgcggg caacagcaag cgatggtcag cgccgagcac caatcgtacg
     3241 gcatgaggaa ccaccatccc gatgaagccg atcatgccgc ttactgaaac gaccgccccg
     3301 gtgacgagcg cggcggtcat caagatgcct cgtttcgccc gttcggtatc aacgcccagc
     3361 gagcgggccg gctcctctcc taatgtcaag atgttcagta cgttgacctg tttgaacaac
     3421 cacgccagac aaagagccaa atagatggag agggccaaca gcgtctggtc ggccgttgcc
     3481 gttagcgatc ccatcaacca cgccatcatg ccgaacgcac gattgggctc catgattgac
     3541 gtgatgaaca tgatgagggc ggcaaaaatg gcatttaaaa tgactcccgc caagaggacg
     3601 ctatggaccg gcagtctgtc gtaggtcgct gacatgcggt agagcaccag gagagccagc
     3661 agaccgccga agaatccata gatcggcaag gtcggaatca gccaggccat gccgcccaga
     3721 ccgaatagga cggccaagga gacaccgagc gcggcgccgc tcgagacgcc gagaatgtag
     3781 gggtcggcta atgggttgcg aagcagcgct tgtagaatca cgccgacgga cgccagagaa
     3841 ctccccacga gaaatccaag caacactcgc ggcatccgca actgcaagac gatcgtgtcc
     3901 gtgatagacg atccattggc cccggtctct cccacaccaa acgctcgtcc gataatccgc
     3961 ccgacttctg caagactcac atactgagtt cccatttgaa gacagaccag cgtgaggacg
     4021 atggcggcgg ttgacagagt ggccatcacg ctgagccatc ggggcatagt gagaatggcc
     4081 ttgcggaagt cggctacatc gccgatagcc gctgcggatt ttggcgatga cgctcgatcg
     4141 gacacggagg atgcgggctg gctgggtgaa attgtcatgg ccgagaagaa gtggggtggt
     4201 cggtagagaa tgcttccgga tgaatcacct gcgccagttg ttccaagccg tcgacgacgc
     4261 gcggtcctgg acgattcagc gcgtcagaag acacgacatg cagccgatga tgttggacgg
     4321 ccgacaaacc ggtccaccgc ttccatgttt gctgttcatg ctcaggaaca ctttcggttg
     4381 accccactgg gaagaccagt atctcaggat ctttctccaa cacggtttcc atgctgagcc
     4441 gtggataggc tcccgacgtt ccagccgcga cattgattcc tcccgccagc tcgatcatct
     4501 gatgaatgta actattggga ccgaccgtaa taagcgggtg gctgcttaat acatataaga
     4561 cccgttttcg ttctacggtc tggacgcggc gcgagacctc ggcgatccgt tcccgcatat
     4621 ggcgagtcag cgcatcggca gcagacgaac gttcgaaaat ccgccccaat gtgtggatgt
     4681 gcgccaggac atgctccagg gatttcgcct caaaaatgaa ggtcggcacc ttcagcgctt
     4741 ccagcttcgc gagcacgtca gcacgcagaa gttcaccggg cgccgctacc atatccgggc
     4801 tcagggccaa caaagattca aggttgggtt gtgtgtagcc gactttgggc ttgccggcgg
     4861 cagccggagg ataattgcaa aaatttgtga cgcccacgat ctgttcgtcg aggccgatgg
     4921 caaacagcat ttccgt
//
LOCUS       DASSCQ010000115         6069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_65006, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000115 DASSCQ010000000
VERSION     DASSCQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65006"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="carB"
                     /locus_tag="VFH05_08050"
     CDS             <1..523
                     /gene="carB"
                     /locus_tag="VFH05_08050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HET6678205.1"
                     /translation="GVDVLLGPEMKSTGEVMGIDADFGWAFAKSQAGAGAMLPMSGMA
                     FISVKESDRPAALQVSKQLHRLGFGIQATSGTAGYLRDHGLEVKTVLKVTEGRPHIVD
                     SIKNGEVALVVNTVGTAASHADSLSIRREALNKGLAYYTTMRGARAAVMGIEAILKKE
                     LTIRSLQEYHCRR"
     gene            540..1016
                     /gene="greA"
                     /locus_tag="VFH05_08055"
     CDS             540..1016
                     /gene="greA"
                     /locus_tag="VFH05_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET6678206.1"
                     /translation="MPTPITKKGYDALKAELDRLRKVERPQVIEAIAEARAHGDLSEN
                     AEYDAAKERQGFIESRIAEIETKLADARVVEIAGRVSDTIVFGATVMVIEQESQAKRQ
                     YTLVGQDEADMKFNRISIQSPVGRALIGKRVGDFIEVKTPVKTVEYEVVEIKFEEF"
     gene            1102..1902
                     /locus_tag="VFH05_08060"
     CDS             1102..1902
                     /locus_tag="VFH05_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET6678207.1"
                     /translation="MPHPSSIITLLTDFGDRDWFVASMKGVILSINPQATIVDLSHRV
                     TSHDVQDGAYLWKSCYRYFPEGTIHVAIVDPGVGSERRPLVAKSSRYYFFAPDNGLLT
                     HVFDQERDVEVREVENKQYRLDSEGHTFDGRDVFAPGAAWLTKNQPMASYGRLVTDAR
                     RFAVAQPHWEQMALVGEVVYVDAFGNLITNVSSGHVKEVREIIKRPNPLIRIVGHSID
                     GLVRCYAEGDRTMPHALINSDGQLEIFCKETSASSLLKVGREEPVKLS"
     gene            complement(1920..3155)
                     /locus_tag="VFH05_08065"
     CDS             complement(1920..3155)
                     /locus_tag="VFH05_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678208.1"
                     /translation="MKRSLTILLLCLTLNLPSLIWASDEQPADRIGQRWATLLAEPVA
                     SPALLSNAQRSGQVIETTVTSTFVRIPSTSTRLRDLSPETDGPRVKGLVAKMSWLKGS
                     LVTESEVANSYGGNDWWEHKIPGDNRVEASRQMVRLGVTGMTGALRYGLNYRKAGQAF
                     LNAPDQSSREMWGEWRANWVTMRSVVGQSWNNVVEDASRPRQVQTYGRMSMGFTRASW
                     PELSLTYGRNSFSSVLEPAGILPQRTQSHTLEGAVAYQSLRWNVRLSSTYALASDLLH
                     GGAESNVRMQLLSASFRPLNTLMISPMLGYREEIQDASGMRIDGPSASLALHYKQSRR
                     LLVSAIGNYANCRSNDGQIDNENVGGKGIVAWDVQQSTKWSTLIALEAGYTRVSNRVT
                     PAADTEDISGLLRIVLAAL"
     gene            complement(3297..4667)
                     /gene="bioA"
                     /locus_tag="VFH05_08070"
     CDS             complement(3297..4667)
                     /gene="bioA"
                     /locus_tag="VFH05_08070"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET6678209.1"
                     /translation="MTRKKQAPSLGVWDRQYLWHPFTQMQEWEEEEPVIIERGKGSYL
                     FDIKGNRYLDGTSSIWVNLHGHRHPALDRALNDQRSKVAHTTLLGLSNPPAILLAREL
                     VRIAPQGLTRVFFSDNGSTAVEIALKMAVQYWKQARPEAGPKHSFVHLKLAYHGDTIG
                     AVSVGNIELFHAPFKPLLFPTQAVDPPYCYRCPLKMTFPECRLACLDPVEQVLRDHRG
                     ELAGFIIEPLMQAAAGMITQPPGYLKRIRELCTKYEVLLIADEVATGFGRTGAMFACQ
                     QEGVTPDLMAISKGLTGGYLPLAATLTTEDIYRAFLGKYEEFKTFFHGHSYTGNPLGC
                     AVALANLEVFRKEQTLGKLRSKIRLLTRLLTPLRRLPHVGEVRQQGFMVGIELVQDRS
                     TKEAYPLETRIGHRVAQEARRRGLLLRPLGHVIVLMPPLSVSVDELNRMVSIVSRSIA
                     AVTQHE"
     gene            4823..>6069
                     /gene="lepA"
                     /locus_tag="VFH05_08075"
     CDS             4823..>6069
                     /gene="lepA"
                     /locus_tag="VFH05_08075"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6678210.1"
                     /translation="MQSLIRNFSIIAHIDHGKSTLADRFLEATGAVTAREAKDQILDA
                     MDLERERGITIKAHAVAIRYKAKDGNTYALHLIDTPGHVDFTYEVSRSLAACEGSLLL
                     VDATQGVQAQTIANVNLAMANNHVIIPVINKIDLASADVENTKHSISEVLMLEASHAI
                     PISAKEGRGVPEVLEAIIERIPPPSGDPHAPLKALIFDSWFDNYQGVIVLTRVVDGAV
                     RPGMKIKVMSNGRTFEVMEVGHFTPKRIKKGELLTGEVGYLCANMKEVADVKIGDTIT
                     DAVQPTAEPFPGYKEVKPLVFCGLYSTDTAKYEDLRDALIKLRLNDSSFVYEPETSLA
                     LGFGFRCGFLGLLHMEIIQERLEREYGLTLITTAPTVVYRVTTTKGEVLEIDNPAELP
                     PPSSIERFEEPFILATVITPERY"
BASE COUNT         1320 a         1683 c         1788 g         1278 t
ORIGIN      
        1 cggcgtcgac gtcctgctcg gcccggagat gaagtcgacc ggcgaggtca tggggatcga
       61 cgcggatttc ggatgggcct tcgctaaatc gcaggccggt gccggcgcga tgcttccgat
      121 gagcggaatg gcgtttatca gtgtcaaaga aagcgaccga cccgctgcgt tacaggtatc
      181 gaaacagctc catcggctcg gatttggaat tcaggcgacg agcggcaccg ccggctattt
      241 gcgcgaccat ggcttggaag ttaaaacggt actcaaggtc acggagggaa ggccacacat
      301 cgtggatagc attaagaatg gcgaggtggc cctcgtcgtc aataccgtgg ggactgccgc
      361 gtctcatgcc gattcattgt ccatcaggcg ggaagcgttg aacaagggat tggcgtatta
      421 cacgaccatg cgcggcgcgc gggccgctgt gatgggcatc gaagccattt tgaaaaagga
      481 attgacgatt cgctcactcc aagagtacca ctgcaggcgc tagaggaacc aaaggaacta
      541 tgccaacacc gatcacgaag aaaggctacg atgcgcttaa agccgagttg gatcgtctgc
      601 gcaaggtcga gcggccacag gtgatcgaag ccattgcgga ggcacgggcg cacggggacc
      661 tgagtgaaaa tgcggaatat gatgccgcga aggagcggca aggattcatc gagtcgcgca
      721 ttgcggaaat tgaaaccaag ctggctgacg cccgggtcgt cgagattgcc gggcgtgtgt
      781 cggacacgat cgtgttcggc gccacggtga tggtcatcga gcaagagtcc caagccaaac
      841 gccaatatac gctcgttggg caggatgaag ccgatatgaa gttcaaccgc atctccatcc
      901 aatctcccgt cggtcgagca ttgatcggaa agcgtgtcgg cgatttcatc gaagtcaaga
      961 cgccggtcaa gacggtcgag tatgaagtcg tggagatcaa atttgaggag ttttagcgag
     1021 cggactgtct cgtgggtgcg tgcagtcacg gcgccaccga ttgcaggaat tacgtagctg
     1081 tctatccatt aggcggtctc catgccgcac ccttcttcga tcatcaccct cttgacggat
     1141 ttcggcgacc gcgattggtt cgtggccagc atgaagggcg tcatcctttc gatcaaccct
     1201 caggcgacca tcgtggacct gagccatcgg gtgacctctc atgacgtcca agatggcgcg
     1261 tacctctgga agtcctgcta tcgatatttc cccgaaggca ccatccatgt cgcaatcgtt
     1321 gatcctggtg tgggaagcga gcgacgtccc cttgtggcaa agagttcacg gtattacttt
     1381 tttgctccgg acaacggctt gcttacccat gtgttcgatc aggaacggga cgtcgaagtc
     1441 cgggaagtgg aaaacaagca gtatcgcctt gattccgagg ggcacacgtt tgacgggcgc
     1501 gatgtcttcg cgccgggagc cgcgtggctc accaagaatc agccgatggc ttcgtatggg
     1561 cgactcgtga ccgatgcgcg caggttcgcg gtggctcagc cgcattggga acagatggcg
     1621 ctcgttggag aggttgtcta cgtggatgca ttcggcaatc tgatcaccaa tgtgtcctca
     1681 ggccacgtga aggaagtgcg agaaatcatc aaacggccga atccgctgat ccggatcgta
     1741 gggcactcta ttgatggact cgtccgatgc tacgccgagg gcgaccgtac gatgccccat
     1801 gctttgatca acagcgatgg tcaactggaa atcttctgca aggaaacaag cgcgagttcg
     1861 ctgctcaagg taggccgaga ggagcccgtc aagctttcgt aggagccgtc ctcaacggtc
     1921 tagagcgcgg cgaggacgat tcgtagcaat ccagaaatat cttcagtatc agcggcgggc
     1981 gtgacccggt tgctcacgcg agtgtagccc gcttcgagag caatcaaggt gctccatttt
     2041 gtcgactgct gcacgtccca ggcgacaatg cccttacctc ccacattctc attgtcgatc
     2101 tgtccgtcgt tcgaacggca attggcatag ttcccgatgg cgctgaccaa gagccgacgg
     2161 ctttgcttat aatggagcgc gagcgaagcg gaagggccgt cgatgcgcat gccggaagcg
     2221 tcctggattt cctcccggta tcccagcatg ggcgagatca ttaacgtatt gagtggtcga
     2281 aacgaggcgc tgaggagttg catgcggacg ttgctttcag ctccgccgtg gagcaggtcg
     2341 ctggcaagag cataggtgga gctgagcctc acgttccacc gcaggctctg ataggcgacc
     2401 gcgccttcga gcgtatgact ctgagtgcgc tgcgggagaa tgcctgccgg ttcaagcaca
     2461 ctggagaagg agttccgccc ataggtgagg ctcaactccg gccatgacgc tcgtgtgaag
     2521 cccatgctca tacgcccata ggtttgaacc tgtcgcgggc gtgaagcatc ttctacgaca
     2581 ttgttccacg actgtcccac gacgctgcgc atcgtgaccc agttcgctct ccattctccc
     2641 cacatttccc gactgctttg gtccggggca ttcaggaagg cttgaccggc ctttctgtag
     2701 ttcaagccat acctgagcgc gcctgtcatc cccgtgacgc caagccgcac catttgtctc
     2761 gaggcttcga cccggttatc gcccggaatc ttgtgctccc accagtcgtt tcctccataa
     2821 ctgttcgcga cctccgattc agtcacaagg cttcccttga gccagctcat tttggccaca
     2881 agtcccttga ctcggggacc atctgtctcg ggggagagat cgcgcaggcg tgtggacgtc
     2941 gatggaattc gcacgaaggt tgaggtgacc gtcgtctcaa tgacctgccc ggagcgttga
     3001 gcattggaga gcagggcggg tgatgcgact ggctccgcca ggagggtcgc ccagcgctgt
     3061 ccgattcggt cggctggttg ctcgtcgctg gcccagatca aggaaggaag gttcagcgtc
     3121 agacacagca acaggattgt cagcgaacgc ttcatgtcga ccccttgaag agccgtaatc
     3181 actctaaaag gtagcagtcg aagattaggg tctaaatgcc ggaaaacagg agtgattttg
     3241 gtgtgaaacg cgtcggttcc gacgagggtg aaacgatgcg gtggaggaac gaggggctac
     3301 tcgtgctgag tcacggcggc gatcgatcgg gagactatgc tgaccatgcg gttcagctca
     3361 tcgacgctga cagacaacgg tggcatcaac acgatgacgt ggccgagcgg cctgagaaga
     3421 agacctcgcc ttctggcctc ctgtgccact cggtggccga ttctggtttc gagcggatag
     3481 gcttcttttg tcgatcggtc ttgtaccagt tcaattccga ccatgaagcc ctgctgtcga
     3541 acctctccga catgaggcag ccgacggaga ggcgtgagca gtcgggtcag cagtctgatt
     3601 ttggagcgca atttccccaa ggtctgctct ttccggaaca cttccagatt ggctagcgct
     3661 acggcgcacc cgagcggatt gccggtgtaa ctatgtccat ggaaaaatgt tttaaactct
     3721 tcatacttcc ctaaaaaggc ccgatagata tcctccgtcg tcaacgtcgc ggcaagcggc
     3781 aggtagcctc ccgtcaaccc tttgctgatc gccatcagat cgggggtcac gccttcctgc
     3841 tgacatgcaa acatagcccc ggtccggccg aatcctgtcg cgacttcgtc ggcaatcaac
     3901 aagacctcgt atttcgtgca gagctctctg atacgtttca agtatcccgg tggctgtgta
     3961 atcatgcctg ctgcggcctg catgagcggt tcaatgatga agccagccaa ttcgcctcga
     4021 tgatcacgca acacttgttc gacagggtcg aggcaggcaa gccggcactc cggaaacgtc
     4081 atcttgagcg ggcagcggta gcagtatgga gggtcgaccg cttgggtcgg gaagagtaag
     4141 ggtttgaatg gcgcatgaaa cagttcgatg ttcccgacgc tgacggctcc gatcgtgtcg
     4201 ccgtgatagg cgagttttaa gtgcacaaaa ctgtgtttgg gtcctgcctc cggacgcgcc
     4261 tgtttccagt attgcacggc catcttgagg gcgatctcga ccgccgtcga accattgtcc
     4321 gagaaaaata cccgtgtcag tccttgcggg gcaatccgca ccagttcgcg tgctaagaga
     4381 attgccgggg gattcgagag gccgaggaga gtggtgtgcg ccaccttgct gcgctggtca
     4441 ttgagggcac ggtcgagcgc cggatgacga tggccatgga gattgaccca aatggacgag
     4501 gtgccatcca aatatctgtt gcccttgatg tcaaagagat acgatccttt cccgcgttcg
     4561 ataattaccg gctcctcctc ttcccactcc tgcatttggg taaaagggtg ccacagatat
     4621 tgccggtccc atacgcccag cgaaggcgcc tgttttttgc gggtcatgcc agttgctcgg
     4681 cggatgcagt tgagaaggta atgcctgaaa acctagagcc cgtgcggtcg cggcgcggat
     4741 tatacgaggg cctctttact ttgacaacct gggagtcagt tactataatg aaccgatttt
     4801 attgaagaaa tgagcgacag atttgcaaag cctcatacgc aatttctcga ttatcgccca
     4861 tatcgatcac ggtaaatcaa ccctcgctga ccggttcctc gaagccacag gcgctgtcac
     4921 ggcccgagag gccaaggacc agatcctcga cgccatggat ctggagcgtg agcggggaat
     4981 tactatcaag gcgcatgccg tggcgatccg ctacaaagcg aaagacggga acacgtacgc
     5041 cttgcacttg atcgatacgc ccggccacgt cgattttacc tatgaagtct ccagaagcct
     5101 ggcggcttgc gaggggtcgc tgcttctggt tgatgccacc caaggcgtcc aggctcagac
     5161 aatcgccaat gtcaatcttg cgatggcgaa caaccatgtc atcatcccgg tcatcaacaa
     5221 aatcgatttg gccagtgcgg acgtggagaa caccaaacat tccatctccg aagtgctgat
     5281 gctggaagca agccacgcca tcccaatcag tgcgaaagag ggccgtggag tccccgaggt
     5341 gctcgaagcc atcatcgagc ggattcctcc cccctcaggt gatccgcacg caccgttaaa
     5401 ggccctcatc ttcgattcct ggttcgacaa ctatcagggc gtgatcgtgt tgacccgcgt
     5461 cgtggacggg gcggtgcgtc ccggaatgaa aattaaagtc atgtcgaatg gtcggacctt
     5521 cgaggtcatg gaagtgggcc acttcacgcc gaagcggatc aagaaaggtg aattgctgac
     5581 gggagaagtc ggctatctct gcgcgaacat gaaggaagtg gccgacgtga aaatcggcga
     5641 caccatcacc gacgccgtcc agccaactgc cgagccgttc cccggctaca aagaagtgaa
     5701 gccattagtc ttctgcggcc tgtattccac cgacacggcg aaatatgagg acctgcgaga
     5761 cgcgctcatc aagctgcggc tcaacgattc gtcgttcgtc tacgagccgg aaacctcctt
     5821 ggccctcggc ttcgggttcc gctgcggttt cctcggtctc ctccacatgg aaatcatcca
     5881 ggagcggctc gagcgggaat acggcctgac cttgatcacg acggctccca ccgtcgtata
     5941 ccgtgtgacg accaccaagg gagaggttct ggaaatagac aatccggcgg agctgccgcc
     6001 tcccagcagc atcgagcgat ttgaagaacc gttcattctg gcgacggtca tcacgccgga
     6061 gcgctacat
//
LOCUS       DASSCQ010000116        17125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_390189, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000116 DASSCQ010000000
VERSION     DASSCQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 17125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17125
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390189"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFH05_08080"
     CDS             <1..914
                     /locus_tag="VFH05_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678211.1"
                     /translation="SWREDGGAIRISSDAKKSTSVEPASTSHSTTASLEWFAAAWVFG
                     VFLFFTLSSTRLAHYIGPLFPAAALLTALYWHRSLHDVQTRGRRASIHTMMIMGFILA
                     IGFASLPSLYPSFAGRLVKEFPLATTMTLGSGPYVASTVLLVGMALVGYFGLSERRRA
                     GAFWAAGGSLALLALTVIQLVIPGLNRYFITPPQILSYAAGLNLTPTDRLIVYGSTRP
                     SNVFYARRKVLFVAEGEEATIREALSQPGQTMVVLPETFKSKLPAEAATLVPLLRQHG
                     YLLLASRSMVAIPEGTASPPARANPGH"
     gene            complement(917..2017)
                     /locus_tag="VFH05_08085"
     CDS             complement(917..2017)
                     /locus_tag="VFH05_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6678212.1"
                     /translation="MNGASDRTALEPSRLLAAPYASILSIAMCCAALALTLLRLPEFD
                     FPLTRYVRSLNDHYIDHLRHPWLAWLSDVGDQVGGSEFLLCVSALLLAVGYTYKLNLW
                     KIAGWDTLVVHAVAGLVSTALKHFVGRARPKFMHVGVSEFSPFAGNGWDSFPSGHTTC
                     SFAIATVLAVRFPKLRWGVFGIALAISASRLFRGSHFLTDIIAGAVLGVLIAVVVVHR
                     HEWRTALGSALYAITPPLAVLLAVITAIGHPLSDEWAARALTEAGLLLAWLALLILLL
                     MKVRPALFPIRATRAVVLAMIGLGLGMCSGSVWVTTVALLTGFAHGLRAGRQGRPDEP
                     PHFPWYREAALGLGLLLSLYTMLELRGALPLG"
     gene            complement(2017..3138)
                     /locus_tag="VFH05_08090"
     CDS             complement(2017..3138)
                     /locus_tag="VFH05_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678213.1"
                     /translation="MTLSLLTRALLQTIVLMATCLHDLSAGEPSMKPNLTGGTERHLK
                     NIRQLTFGRQNAEAYFSFDGTKLIFQSTNNWMKDSFAATLKPAEEGLGCYQMYVMDLE
                     SSAIRLVSTGTGATTCGYFFPGDRRVLYSSTYAAGPNCPPKPKRDGAYRWALDDYDVY
                     AVRLDGQEMQRLTSTPGYDAEATVSPDGKTIVWTSMRDGDLDLYAMNLDGTKSRRLTE
                     EVGYDGGAFFSPDSKRIVYRASHPRDDNELSRYKELLGQRLVEPGQLEIFVMNADGSG
                     KRQVTSNGASNFSPYFHPDGKRIIFSSNVETRGEGGRPSFHLFLVTDDGTGLERLTVE
                     GHFNSFPMFSPDGTRLVWVSDRHAKAPGEFNVFLADWVP"
     gene            complement(3116..5332)
                     /locus_tag="VFH05_08095"
     CDS             complement(3116..5332)
                     /locus_tag="VFH05_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET6678214.1"
                     /translation="MAALWLLFCLNSSVLTTTATPQPVSASVISRHDLFVQIDPERHA
                     LLAADRLTIEAIPGRPLRLSLAGTLHLDRLALSQSTTDDIGRDLPFQIEREATAPFGQ
                     YITLPAALVTAGAMTLTAHYHGVIDDPPKEPRHLRFVTPSETAGHIGAEGVYLSSETQ
                     WYLDFPESLSRYRLRVALPSGWTAVTQGQRRSSAPCPAELCREAGLTLAEWETAEVSE
                     ALTLVANRFVETSREWASSGGQSVRLATYLFPEDAGLADEYLDATARYLDAYISLLGP
                     YPFESFAVVENFFASGLGMPSFTLLGSGVIKRHYVQPYALGHEIVHSWIGNSVFNRVD
                     RGNWVEGLTTYLANYYWHEWSGDHAQARDQRHLMLRGYNLHVPPERDYPLGQFTRKQD
                     ERDNAIGYQKAAMVFHVLRQEVGEESFWRSLKTLVAQYRGRHADWHDVERIFAETAAK
                     DLRWFFAQWIEGTGAPALTLKNVTAHPLPRDADGRFSLSGSIAQTGPVFRSPLPILVR
                     MSNSRQHLVSTQLSGADTLFKSDLPFKPLTIELDPEAMVLRRLARRDMPPVLNHYVTD
                     TKRSVILAFGGSDTDSHPFQDVVKRIRSQDAQKAPGQGTAITTLAENALLPGEGSVLI
                     LGGPASRLTLQPLLSTHCGTRLQLRDGGVTVAGKAYDGPGIALVASCHREDRPGSVVT
                     SVYALTPQSATAVARLLFFYGWNSYVVFQDGKPIARGEWDAEHSQEVSLDDTQSAH"
     gene            complement(5370..6152)
                     /locus_tag="VFH05_08100"
     CDS             complement(5370..6152)
                     /locus_tag="VFH05_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678215.1"
                     /translation="MVKILVIDDDRMNCDLLQAVLTRHGYDVHSATSGLEGLNLFREH
                     HPRVTILDLRMPEMDGLTVLKEIRAIDPHAPVIILGGGATESQENQARTLRVTDFVRK
                     GLSLDVLVEGVNRVVQQPAKKIEGATAQSGNAVVDTGETVLIVDDEQLVCDLLVQFLS
                     LRGYRALGVKDGAQALSMIEQTRPDLILLDLMLPGMNGVEVLRRLRDNNFAGAVIIVT
                     GSYDEELLQDAWSLRPQEVISKPIDLDKLLSIIQLVLVCREC"
     gene            complement(6254..7183)
                     /gene="ftsY"
                     /locus_tag="VFH05_08105"
     CDS             complement(6254..7183)
                     /gene="ftsY"
                     /locus_tag="VFH05_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET6678216.1"
                     /translation="MGWFQRLSEGLTKTRSVMRGSLDRLLGRAVDPALLDEFEAALIT
                     SDLGAAVAERVISRLKETLQGTDASQATHVHATLRQTLLEILTPASGPTFEQLIANGP
                     KPFVVLVVGVNGVGKTTSIAKLARRLVQSKRVPMLVAADTFRAAAIDQLQVWADRIGI
                     EMIRHRHGADPAAVAYDGIAAAKARQADVVLIDTAGRLHTKSNLMDELRKVSRVIGQA
                     SPGAPHEVLLVLDATLGQNALSQARQFHQTVGVTGLALTKLDGTAKGGIVVAIADELK
                     LPVRLIGVGEAVDDLQDFQPAAFVDALLGAATD"
     gene            complement(7199..7708)
                     /gene="ybeY"
                     /locus_tag="VFH05_08110"
     CDS             complement(7199..7708)
                     /gene="ybeY"
                     /locus_tag="VFH05_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET6678217.1"
                     /translation="MGPAALMPVSLQVRVRSIRFGRQSVLRLAQAVLDLIGESSADLG
                     LSFVGDRRMRSLNHRFRGKDRSTDVLAFALREARTPLSRVSRQVLGDIVISVPTALRQ
                     AKEGRRSLEEEIVALLIHGALHLCGYDHERGHAESRRMRRKERTVRLALGRIRCFTGR
                     RPDVRNSSE"
     gene            complement(7690..8745)
                     /locus_tag="VFH05_08115"
     CDS             complement(7690..8745)
                     /locus_tag="VFH05_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET6678218.1"
                     /translation="MRKLKLREGTNTAVLFGHHDRHLKLIEEDLGVRLSARGEELTLD
                     GNPEATRYAERVLTELAGLANDGMLIQPDDVTHALTALRQSPETPLKELLSNATAIVT
                     KKRFVAPKTPTQKTYIEAIETHDIVIGIGPAGTGKTYLAMAMAVSALMKREVSRIILA
                     RPAVEAGEKLGFLPGDMIAKVNPYLRPLYDALFDMMDMERATRAIERGDIEIAPLAFM
                     RGRTLNDSFVILDEAQNATAEQMKMFLTRLGFNSKAVVTGDITQVDLPPDRVSGLIEV
                     RDILRHVEGIRFVYFDERDVVRHKLVQDIIKAYDQHEQTEQTHPQAAQAGRRQSSHTR
                     SSSSIPGTTKGSWGQPH"
     gene            complement(8936..10759)
                     /locus_tag="VFH05_08120"
     CDS             complement(8936..10759)
                     /locus_tag="VFH05_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET6678219.1"
                     /translation="MEGSHVSVSADTASVEADRKKAGPGLKSRPFSIEQNVLDLLVCR
                     GILSQAVLDRAAQEAAESGDDLESLLLDRFQVPKTVLGAAMSDYYQCPYLPYDERTVI
                     ASELLRPLNADYLKKHLWLPVARRGQLLDVLAHDPHDLERGWDVRRSFPGMTIRYSVG
                     LRRDIEQFLGATAEQQGGASTIGTILNELEEDLPLDPVHERPSGPIDENDSLIVRLTN
                     HLISEADRLGASDLHFEPSTGKREMNIRFRVDGTCFSYTKVPAAYRRALISRLKIMAN
                     LDIAERRKPQDGKIRFKLDAQRSIELRVATLPTGGGNEDAVLRLLTAKEPMPLEAMEL
                     TPSALQTIKAMAEQPHGIILCVGPTGSGKTTLLHAILKHINTEERKIWTAEDPVEITQ
                     EGLRQLQIHQKIGLDFATAMRAFLRADPDVIMIGEMRDKETADIAIEASLTGHLVLST
                     LHTNSAVETVIRLLDLGCDPFNFADAMLGVLATRLCKRICSVCKESYRPSPHEYQELV
                     ESYGAEGWPLLQTGQDDLHLFRGRGCEHCRHSGYKGRVPLHELLVGTDEIKQLIQSRS
                     RTTAMLIKAVEDGMLTLLQDGIRKVLQGLTTYRQVRAVASK"
     gene            complement(10772..12235)
                     /locus_tag="VFH05_08125"
     CDS             complement(10772..12235)
                     /locus_tag="VFH05_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678220.1"
                     /translation="MKIRRHPLVENRGHVLLASLMLVIMLSLLSMTALYLVGQDAPGI
                     SAMREHGQAQQLADAATELVVSWFHDASTTPASIADLLTKRRADATGAPSFFDTDGRS
                     QFTGTPDRPDLLLEAVNEDDDRILNRAPSGFSGPLSEMGRLEMIKLYAPVEPGLLGTV
                     EVTASARGSRPTASTIRFQLGAVDLPPVRAAVQTGQGLSVSSPETERPLFVHWGDTRV
                     MGDMAINRMDDLVVKSTTASINGQSYDTMGKVEDRWVDYWIGGNLSVLSTPDGTMSVP
                     QNVHTRQEPTPGARLDRWDYDLLKKTAQRYGTYYRLDRDGRLHELGSIESDPGRSPSE
                     LLVSLTVGQSHGLVFIDTLDGETPRSDNLGTLVVDGDYVEALLVVQGHVLVKPSGPGR
                     SVTVLSPPPEGTSALSGRIPVTLPGIHLNGMLYAAGNITVERQTRIFGAVMTSGTLTG
                     GTRPPSLEIWYNWDFSKGLFRGLPVVYRASSTWQPKY"
     gene            complement(12255..13001)
                     /locus_tag="VFH05_08130"
     CDS             complement(12255..13001)
                     /locus_tag="VFH05_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678221.1"
                     /translation="MQTKSAAKRSCVRMPLINQFGSSLVELLAAMAAGLVVLGAALQS
                     FSYFHQQFLMQRDTILQAQDVRLSLELLAQELHLASIGSLSVARPDAVEFTANISGIM
                     TKVTAPGLMGETTLSVEDGRGWPDHKIVGVCWNERCDSFTLARAGLRSSLAFVEPIVR
                     PIPAGASASVKNRVRYYSRLDENGILRFLRQVDGGAGVLVGHVESIRFSYWDAQGRMT
                     AHPGLVRRVIVEITAPQRAATGKREITLRT"
     gene            complement(13013..13504)
                     /locus_tag="VFH05_08135"
     CDS             complement(13013..13504)
                     /locus_tag="VFH05_08135"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HET6678222.1"
                     /translation="MTGWSRCRSIAGATFSEVLVAMALTSVGLVATMGALQAAYQQFG
                     RGVLATRAMEMADSKLEAKRAVRWDQLLTEDFDQNGLPESLMRDDGTAGDRVAGDGIY
                     SASYEQNGVTLIWTVAPNQGGNLALSGYVLLEARASYRSHDGDHELRAVTLRANAVLA
                     GHH"
     gene            complement(13501..13968)
                     /locus_tag="VFH05_08140"
     CDS             complement(13501..13968)
                     /locus_tag="VFH05_08140"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HET6678223.1"
                     /translation="MRQWKHGGATRLSASSGRSLLELVVVLALVGIMASMAGPSFQSL
                     NARLQARTATAEIASTLRMARQLAMARRERLLMRFDLPGKTISLQRADADAILTVYRY
                     GDKGIVVDEPTAGCELLFHPSGRSATASTIVIHDRDNRRTTITVSLSGRVVTS"
     gene            complement(14211..14993)
                     /locus_tag="VFH05_08145"
     CDS             complement(14211..14993)
                     /locus_tag="VFH05_08145"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET6678224.1"
                     /translation="MIDGSFYLLAAGFGALIGSFLNVCIHRLPRCESIVWPGSHCPSC
                     GTTIAYYDNIPLFSYLWLMGHCRTCRSSISMRYPVVEAVNAAGYVMILATFGLTWTTV
                     LYSALFSALLVVTGTDLTHRIIPNVVTVPGIVIGLLGAATVLPVGLMNALLGVAIGGG
                     ILWALAWASPYLFGKEGMGGGDIKLLAMIGAFLGWKPALLTIMVGSLSGSLIGLSLIG
                     LGLMKRDEYIPFGPFLVFGALVSMFFAQPLLEWYQTLLTPSG"
     gene            15201..15476
                     /locus_tag="VFH05_08150"
     CDS             15201..15476
                     /locus_tag="VFH05_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6678225.1"
                     /translation="MARIARVKSRTSLADLAPPPRKKRPESLTSREQEILELIWAGFK
                     NKEIGQRLKISVKTVEAHRANMMKKMRVSNTAQLLKTAIQGNIIRVR"
     gene            complement(15460..16212)
                     /gene="rlmB"
                     /locus_tag="VFH05_08155"
     CDS             complement(15460..16212)
                     /gene="rlmB"
                     /locus_tag="VFH05_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET6678226.1"
                     /translation="MYGLHAVREALRAGTRPLQRLLVLRTDRQFSDLVHQARSQHVPV
                     HIEPPAAFDRLVPDQRHQGIIAFIAAKGYCSTDEILEHARRLDEPPFLVILDGVEDPH
                     NLGAVLRTAEAAGVHGIFIPERRAVGLTPVVAKVSAGALDHMRVARVVNLSRLIEELK
                     GDGVWVYGVEPSAPISVTTIDMTGPVAFVFGGEGGGIRHGVLQECDGRVRIPMKGKVA
                     SLNVSAAVAVTLFEGVRQRSAPAQIQRPIEPE"
     gene            complement(16226..>17125)
                     /locus_tag="VFH05_08160"
     CDS             complement(16226..>17125)
                     /locus_tag="VFH05_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HET6678227.1"
                     /translation="WDSPWGPGRPGWHIECSAMSIKHLGETFDIHGGGMDLIFPHHEN
                     EIAQSCGATGKEFANYWVHNGFVQINQEKMSKSLGNFFTIREIFEKETQQWPEAIVGE
                     ILRYFLLSTHYRSPLDFSDQSLHEAKNALDGFYDLFKRLEEGQQGTTLDEEVRTIIDR
                     ARLAYRQKLDDDLNTPMALAVLQNMRSNVNKRLERWDSIESRKRIRAAFREFGGVLGL
                     FQLDSEQWQFGKTVGLTGVEATVSAGQVSVLKTTEIERLLGERLEARKRKDFAKADEI
                     RKSLAAQGIIVEDKPDGTTRWKR"
BASE COUNT         3721 a         5079 c         4850 g         3475 t
ORIGIN      
        1 gaagttggcg agaggacgga ggcgcaatac gcatctcgag cgacgcgaaa aagagtactt
       61 cggttgaacc cgcttcaacg tctcacagta caacagcaag cttggaatgg ttcgcggcgg
      121 catgggtctt cggcgtcttc ctgtttttta cgttgtcgtc gaccaggctg gctcactata
      181 tcggaccact gtttccagcc gccgcgctgc tcaccgcact ttactggcac cggagcctcc
      241 acgatgttca gacgagaggt cgacgcgcct cgatccatac catgatgatc atgggcttta
      301 ttttggccat tggatttgcc tcgttgccgt ctctgtatcc ttcgtttgct ggacgtctgg
      361 tgaaggagtt tccgctcgcg acgacgatga ctctcgggag cgggccgtat gtcgcctcca
      421 cggtcctgtt ggtcgggatg gcgctggtcg gctactttgg gttgagtgaa cgaagacggg
      481 ctggggcatt ctgggctgcg gggggctcac tggcactgtt ggcattgacc gttatccaac
      541 tggtcatacc cggtcttaac cggtatttca tcacgccgcc ccagattctt tcatatgctg
      601 ccgggctcaa tctgacgccg accgatcgct tgattgtgta tggatcgacc agaccttcaa
      661 acgtgtttta tgcgagacgc aaagtgctct tcgttgcgga aggagaagag gccacgattc
      721 gtgaggcgct cagccagcct ggtcagacta tggtggtcct gccggagacc ttcaaatcca
      781 agctgccggc cgaagccgcg acccttgtcc ctctcctgag acaacacggc tatctcttgc
      841 tcgccagtcg gtcgatggtc gccattcctg aaggcaccgc ctcgcctccg gctcgagcga
      901 atcccggcca ttaaagctat cccaacggca gggcgcctcg taattcaagc atcgtataca
      961 aggacagtaa cagccccaga cccagtgccg cctcgcggta ccaggggaag tgaggcggct
     1021 cgtccggccg tccctgtcga cccgcgcgca acccatgcgc gaagccagtc aggagggcga
     1081 ccgtcgtcac ccacacggaa cctgagcaca tgccgaggcc aaggccgatc atcgcaagca
     1141 caacggctcg ggtcgcacgg attggaaaga gggcgggccg taccttcatc aagaggagta
     1201 tgagcagcgc caaccaggcc agcaacaatc ccgcctcagt caacgccctt gcagcccact
     1261 catcggaaag ggggtgcccg atggcggtga tgaccgcaag aagcactgca agcggcggcg
     1321 tgatggcata cagcgccgat cctagtgccg tgcgccattc atgccggtga acgacgacaa
     1381 cggcgatcag cacgccaagg acggcacctg cgatgatgtc ggtcaagaaa tgtgaccccc
     1441 ggaagagccg gctcgcggaa attgccaacg cgatgccgaa cacgccccaa cggagcttgg
     1501 ggaatcgtac cgccagcacc gtggcgatgg cgaaggagca ggtggtatgg ccggatggaa
     1561 acgaatccca accattgccg gcaaacgggg aaaattcaga gacacccaca tgcatgaact
     1621 tgggcctggc acgacctaca aaatgcttga gggcagtact gactaggcca gccacagcat
     1681 gaacaacgag cgtgtcccaa ccggcgatct tccaaagatt gagcttatac gtgtatccga
     1741 ccgccaagag tagagcactc acgcacagca ggaattcact gccgcccact tgatcgccaa
     1801 cgtcactgag ccatgccagc catggatgac ggagatggtc aatgtaatgg tcattgaggg
     1861 aacgcacata gcgagtaagg gggaaatcga actcaggcag gcggagtaag gtcagcgcaa
     1921 gtgcggcaca acacatcgcg atcgacagga tcgacgcgta gggtgccgcc aacagtctcg
     1981 acggttccaa tgccgtacgg tcagacgcgc cattcatcac gggacccagt cggcgagaaa
     2041 cacgttgaat tcaccgggag cctttgcatg acggtcggac acccagacca gccgcgtccc
     2101 atcgggagaa aacatgggaa aactgttgaa gtgcccttca acggtcaggc gttccaagcc
     2161 ggttccgtca tcagtgacga ggaatagatg gaaacttggt ctgccaccct cgcccctggt
     2221 ctcgacgttg gatgagaaga ttattctctt gccgtccgga tgaaaatagg gtgaaaagtt
     2281 agaggcccca ttcgaggtaa cctgacgctt cccgctccca tcggcattca tgacaaagat
     2341 ttccaattgg ccaggttcga ccaaccgctg tccgagcaac tctttgtaac gtgacagctc
     2401 gttgtcatct cttggatgag acgctcggta gacgatgcgt ttgctgtcag gcgaaaaaaa
     2461 cgcgccgccg tcatacccga cctcctcggt cagacgacga gatttggttc cgtccagatt
     2521 catggcatac agatcaagat ctccatccct catggaggtc cacacgatgg ttttcccgtc
     2581 cggcgagacg gtcgcttccg catcgtagcc tggtgtggag gtcagacgct gcatctcctg
     2641 accgtcaagg cgaaccgcat acacgtcgta gtcgtccaag gcccatcggt aggcgccgtc
     2701 tcgtttgggt ttcggcgggc agttcggtcc cgccgcgtat gtggaagaat agaggacacg
     2761 ccgatctcct gggaagaaat acccgcacgt ggtggcgccg gttcctgtgc tgaccagccg
     2821 tatagcgctg ctctcgagat ccatcacata catctgatag cagcccaagc cctcttcggc
     2881 cggcttgagc gtcgcagcaa aggaatcctt catccagtta ttcgtggact gaaaaatcag
     2941 cttcgtgccg tcaaacgaaa aataggcttc cgcattctga cggccgaagg tgagctgccg
     3001 gatgttcttc aaatgtcgtt ccgtgccccc ggtcagattc ggtttcatcg aaggttcgcc
     3061 ggcggacagg tcgtgcaaac aggtcgccat gaggacgatg gtctgcagca gtgccctagt
     3121 gagcagactg agtgtcatcg agcgaaacct cctggctgtg ctctgcatcc cattcaccgc
     3181 gagcaatcgg tttgccatcc tgaaagacta catagctgtt ccatccataa aaaaacaaca
     3241 gccgagcgac ggcggtcgcg gattgcggcg tcagggcata cactgatgtg acgacgctgc
     3301 ccggccgatc ctctcgatga cagcttgcga caagggctat gcccggaccg tcgtaggcct
     3361 tccccgcgac ggtcacacct ccatcccgaa gctgcagacg agtgccgcaa tgagtactga
     3421 ggagcggctg aagcgtcaaa cgggaggccg gaccccccaa tattagcact gaaccttcgc
     3481 caggcaacag agcgttttct gccagggtcg tgatcgctgt cccctggccc ggcgccttct
     3541 gagcgtcctg actcctgatc cgtttcacca cgtcttgaaa cggatgactg tctgtgtccg
     3601 atccgccaaa agccaggatg accgagcgct tggtgtctgt aacatagtgg ttaagaaccg
     3661 gcggcatatc gcgccgggcc aaacgacgca ggaccatcgc ctcgggatcc agctcgattg
     3721 tcagcggctt gaatggcaag tcactcttga acagcgtgtc cgcaccggac aactgtgtcg
     3781 agaccagatg ctggcggctg ttggacatcc gcacaagaat cggaagcgga gatcgaaaca
     3841 cggggccggt ttgagcgatg ctgcctgata ggctgaagcg gccgtcagca tcgcgaggaa
     3901 gcggatgtgc cgtgacgttc ttcaaggtca atgcaggcgc gccggtgccc tcgatccatt
     3961 gcgcaaagaa ccagcggaga tccttggcag cggtctctgc aaagattcgt tcgacgtcat
     4021 gccagtcggc atgccggcca cgatactgcg cgacaagcgt tttcagcgag cgccaaaatg
     4081 actcttcgcc gacttcctgc cgcagcacat gaaagaccat cgccgctttt tgatacccga
     4141 tggcgttgtc ccgctcatcc tgcttccggg tgaattgtcc aagcggatag tcacgttctg
     4201 gcggcacgtg caggttatat ccccgcagca tcaagtggcg ctgatcccgc gcttgtgcat
     4261 gatcgccgga ccactcgtgc caataatagt tggcaagata ggttgtcaat ccttccaccc
     4321 aattgccacg atccacccgg ttgaaaacgg agttgccgat ccaggaatgc acgatttcat
     4381 gacccaaggc atacggctgc acatagtgtc gtttgatcac tccgctcccc aacagcgtaa
     4441 acgagggcat gcccagcccg ctggcaaaaa agttctcgac cacggcaaac gactcgaacg
     4501 gataggggcc gagcaacgat atgtacgcgt caagataccg agccgtggca tcgaggtatt
     4561 catcagccaa cccggcgtct tccggaaaca ggtacgtggc caaccggacc gactgaccgc
     4621 cgctcgacgc ccactcgcgg ctggtctcta caaatcggtt ggcgaccagc gtcagcgcct
     4681 cgctcacttc ggccgtctcc cactcggcca gcgtcagacc cgcttcgcga cacagctctg
     4741 cggggcaagg agcggaggac cgacgctgtc cttgggtgac ggccgtccag cccgagggca
     4801 gagcgacacg aagccgatac cggctgaggg attcagggaa atccagatac cactgggttt
     4861 cactgctcag gtacacacct tctgccccga tgtgaccggc ggtctcactg ggagtcacaa
     4921 agcgcaaatg acgcggctct ttaggaggat cgtcgatcac tccatgatag tgcgcggtca
     4981 atgtcatggc gccagccgtt acaagggcgg cgggcagtgt gatgtactgc ccgaaaggag
     5041 cagttgcctc gcgctcgatc tgaaacggga gatcacgacc aatgtcgtcg gtggtggatt
     5101 gggacagcgc caaccggtca aggtgaagcg tcccggcgag ggacagtcga agtgggcgcc
     5161 cggggatcgc ctcgatcgtg agacggtctg ccgccagaag cgcatgtcgc tcgggatcaa
     5221 tctggacgaa taaatcatgg cgtgagatga cggaggccga aacgggctgt ggcgtcgccg
     5281 tcgtcgtcaa tacagacgag ttgaggcaga acaatagcca cagagccgcc atggccgctc
     5341 gccggcacga atgttccatg cgttgggttt tagcactcgc gacagacgag cacaagctgg
     5401 atgatggaga gcagcttatc gagatcgatg ggcttactga tcacttcttg gggacgtagg
     5461 gaccacgcgt cttggagcaa ttcttcgtcg tagctccccg tgacgataat gaccgcgccc
     5521 gcgaaattat tgtcgcgtag gcgacggagc acctcgacgc cgttcatgcc cggcagcatc
     5581 aaatccaata aaatgaggtc gggacgagtc tgttctatca tcgacagcgc ctgcgcgccg
     5641 tctttgacgc ccaaggctcg atacccccgc agactcagaa actgcacgag cagatcgcag
     5701 accagctgct cgtcatcgac gatgagcacg gtttccccgg tgtcgacgac ggcattaccg
     5761 gattgagccg tcgctccttc gatcttcttg gcaggctgct gaacgacacg attgacacct
     5821 tctaccaaca catccagcga caatcctttg cggacaaaat ccgtcacgcg caatgtacga
     5881 gcttggttct cctgactctc cgtcgcaccg ccgccgagaa tgatgaccgg cgcgtgcgga
     5941 tcgatagctc gaatttcttt gagaactgtg agcccgtcca tttcgggcat ccgcaggtca
     6001 aggatcgtga cgcgaggatg gtgctcgcgg aagaggttca ggccttcgag gccgctggtc
     6061 gccgaatgaa catcatatcc atgacgagtc aggacggcct gcagcaggtc gcaattcatg
     6121 cggtcgtcgt cgatgacgag gatcttgacc attcccactc cttgcggcaa acggtgtgct
     6181 acaccgcggc acggaccgag cctagcatgg ggactaggac ttgagaagta aaagagctat
     6241 accggccatc tggttagtcg gtcgcagcac caagcaacgc atcgacgaaa gccgccggtt
     6301 ggaaatcctg caaatcgtct accgcttctc ccacaccgat cagccgtacg ggcaatttga
     6361 gctcatccgc aatcgccacg acgatgcctc ccttggcggt cccatccagt ttggtcagag
     6421 ccaacccggt tacccctacg gtctgatgga attggcgggc ttgcgacagg gcgttttgcc
     6481 ccaacgtcgc atctaaaacc agtaagactt catgcggcgc gccgggcgag gcctgcccga
     6541 tcactcttga gaccttccgg agttcatcca tcaaattgga cttcgtatgc agacggccgg
     6601 ccgtatcgat caagacaaca tcggcctgtc gagctttggc cgcggcaatc ccgtcgtagg
     6661 ccactgccgc cggatccgcg ccatgccgat ggcggatcat ctcgataccg atccgatcgg
     6721 cccagacctg aagttggtca atcgcggcgg cacgaaaggt atcggccgcg acgagcatcg
     6781 ggacgcgctt actttggacc agtcgccgcg cgagcttggc tatgctggtg gtctttccga
     6841 ccccattgac tccgaccacg agcacgacaa acggtttcgg tccattggcg atcaactgct
     6901 cgaaggtagg ccctgacgcc ggggttaaaa tttcgagcaa ggtctgacgc aatgtcgcgt
     6961 gaacatgagt ggcttgcgag gcatcggtgc cctgtagggt ctccttcagg cggctgatca
     7021 cccgttcggc cacagcggct cccaggtcgg aggtgatgag ggccgcttcg aactcgtcga
     7081 gcaaagcggg atccactgcc cgacctaaca agcgatcgag ggagccgcgc atgacggagc
     7141 gcgtcttagt cagaccttca ctcagtcgct ggaaccatcc catgaatcga actccgtctt
     7201 attctgatga gttccgaacg tcaggacgcc gaccggtgaa acacctaatc ctgccaaggg
     7261 cgagccggac cgtccgctct ttccgtcgca tccgccggga ttcggcatgg cctcgctcgt
     7321 ggtcataacc gcataagtga agggctccgt ggatcaataa ggccacgatt tcttcctcca
     7381 aggagcgacg gccctccttc gcttgtcgaa gcgcagttgg aaccgagatg acgatgtctc
     7441 ccaacacctg tcttgagacg cgagagagag gcgttcgtgc ttctcgcaac gcaaacgcga
     7501 gcacgtcggt gctgcgatct ttgccacgga agcggtgatt caggctcctc atccgccgat
     7561 cacccacgaa cgacaatccg agatcggcgg aggactcccc gatcaggtcg agtacggcct
     7621 gtgccagtcg caggacgctt tgacgaccaa accgtatcga tcgtacgcgt acctgtagcg
     7681 aaacgggcat taatgcggct ggccccaaga acctttcgtt gtgccgggta tggaggagga
     7741 cgagcgggtg tgggaggact gacggcgccc tgcctgcgcg gcctgtggat gggtctgctc
     7801 ggtctgctcg tgctggtcat acgctttgat gatgtcttgc acgagcttat gccgcacgac
     7861 atcacgctca tcaaaataca cgaagcgaat gccttccacg tggcgaagaa tgtcgcgaac
     7921 ttcgatcaac cctgaaaccc gatcgggcgg aagatccacc tgggtaatgt caccggtgac
     7981 cacggctttg gagttaaagc cgagccgcgt caggaacatc ttcatctgtt cggccgttgc
     8041 gttttgggct tcgtcgagaa tcacgaagga atcattcaac gtgcggcctc gcataaatgc
     8101 caatggcgcg atctcgatgt cccctcgctc gatggcacgc gttgcccgct ccatatccat
     8161 catgtcgaac agggcgtcat agagggggcg gagatacgga ttgactttgg cgatcatgtc
     8221 gccgggcaga aatccgagtt tctctccagc ttcgacggca ggcctggcca gaatgatgcg
     8281 gctgacctcg cgtttcatca acgcactcac ggccatggcc atcgccagat acgtctttcc
     8341 tgttcccgca gggccgatgc cgatgacgat gtcgtgcgtt tcgatcgctt cgatataggt
     8401 tttctgcgtc ggcgtctttg gagcgacaaa gcgtttcttg gtgacgatcg ccgtcgcatt
     8461 ggagagcaac tcttttaacg gggtctccgg actttggcgc aaggcggtta atgcgtgcgt
     8521 gacgtcatcg ggctggatca gcatgccgtc attggccaag cctgccagtt cggtgagtac
     8581 tcgctccgca tagcgcgtcg cttcaggatt gccgtcaagg gtaagttcct caccgcgtgc
     8641 cgataggcgc acgccgaggt cttcttcgat cagcttgagg tgccggtcgt ggtgtccgaa
     8701 cagaacggcg gtgttggtgc cttctcgcag tttgagcttt cgcacgaggg gccgaagccc
     8761 gttcctcctc tcactactaa gtgattctaa caaagcggcg gcagagggac aagcgaaagg
     8821 tcggaagaat aggcgttgcc ctgggacatg ggcctttcca gtctgtacag ggcagggccc
     8881 cggtgacgtg tgagggctgc acggccggtc tggctgacgc gatcggtgcg tcaggctact
     8941 tgctcgccac tgccctgacc tggcggtagg tggtcaggcc ctgcaggact ttgcggatac
     9001 catcctgaag cagagtcagc atcccgtctt caacggcttt gatcagcatt gcagttgttc
     9061 gactccttga ctgaatgagc tgcttgattt cgtcggttcc gaccaggagc tcatgaagag
     9121 gcactcgtcc cttgtaccct gaatggcggc agtgctcaca cccacgacct cggaacaggt
     9181 ggaggtcgtc ttgcccggtc tggagtaagg gccacccttc ggcgccgtaa ctctcaacca
     9241 gttcctgata ctcgtgggga gacggacggt aggattcttt gcagacgctg cagattcgtt
     9301 tacacaacct ggtggcgagc acaccgagca tggcatcggc aaaattaaac ggatcgcacc
     9361 ccaggtcaag cagacggata acggtttcga cggcactgtt cgtgtgcagc gtgctgagaa
     9421 caaggtgacc ggtcagagac gcttcaatcg caatgtcagc agtttcttta tctcgcattt
     9481 ccccgatcat gatgacgtca gggtcagctc gcaaaaacgc gcgcatggcc gtcgcaaaat
     9541 ccaagccgat tttctggtga atttgtagtt gccgcaaacc ttcttgcgtg atctcgacgg
     9601 ggtcttcagc ggtccaaatt ttgcgttctt ccgtgttgat gtgcttgaga atagcatgca
     9661 gcagcgtcgt tttccccgag ccggttggcc ctacgcacag gataatgccg tgcggctgtt
     9721 ccgccatcgc cttgatcgtc tgcaatgcgc tcggggtcaa ctccatagct tccagaggca
     9781 tcggttcttt cgcggtcaaa aggcgcagca cggcatcttc gttgccgccg ccggttggca
     9841 gggtggccac acgcaattca atgcttcgtt gggcgtcgag cttgaatcgg atcttcccat
     9901 cttgcggctt acgccgttcc gcaatgtcga ggttcgccat gatcttgagg cgggagatca
     9961 atgcccggcg gtaagcggct ggcactttcg tgtaactaaa gcaggtccca tcgacccgga
    10021 accggatgtt catttcccgc ttgcccgtgc tcggctcaaa atgcagatca gacgcaccaa
    10081 gccgatccgc ttcgctaatc aaatggttcg tgagccggac gataagggaa tcgttctcgt
    10141 cgatagggcc ggaaggcctc tcgtgcaccg gatcaagagg aagatcttcc tccaattcat
    10201 ttaagatggt tccgatggtt gaagcgcccc cctgctgctc cgccgtggcg ccaagaaatt
    10261 gttcaatgtc gcggcgaagc ccaacggaat accggatggt catgcccgga aaactgcgcc
    10321 ggacgtccca ccctcgttca agatcgtgag gatcgtgagc cagcacgtcg agcagttggc
    10381 cacggcgagc aacgggcagc cacaggtgct ttttcaaata gtcggcattg agtgggcgca
    10441 gtagctcaga agcgatcact gtccgctcat cgtacggaag gtacgggcat tggtaatagt
    10501 cactcatcgc cgcgcccagc acagttttag gaacctgaaa acgatccaag agcaggcttt
    10561 ccagatcgtc gccgctttcg gccgcctcct gtgccgcacg gtccaacacg gcttgagaga
    10621 gaatgccgcg gcagacgagc agatccaaga cgttctgttc aatcgagaaa ggacggcttt
    10681 tcaaacctgg tccggccttt tttctatcgg cctccaccga cgccgtgtcg gctgagactg
    10741 agacgtgaga accctccatc ggatcctcgc atcaatattt cggttgccat gtggaggaag
    10801 cacgatagac gaccggcagg ccgcggaaga gtcctttact gaaatcccag ttgtaccaaa
    10861 tctccaagga gggaggccga gttccaccgg tgagggtacc cgaggtcatc acagcgccga
    10921 aaatgcgcgt ctgtcgttcc accgtgatgt tgcctgccgc atataacatg ccattgaggt
    10981 gaatgccggg cagcgtgaca ggaatcctgc cactcaaggc gctcgtccct tcaggaggcg
    11041 gactcaacac cgtcaccgag cggcccggtc cgctcggctt cacgagaaca tggccttgca
    11101 caaccagcag ggcttcgaca tagtccccat ccacgaccaa cgtaccaaga ttgtcggagc
    11161 gcggggtttc tccgtccaag gtgtcgatga acacgaggcc atgcgattgt ccgacggtca
    11221 gtgagaccaa cagctcggaa ggcgaccggc caggatccga ctcgatcgat cccaactcat
    11281 gaagtcgtcc atctcggtcg agccggtagt aggttccgta gcgctgcgcc gttttcttca
    11341 gcagatcgta gtcccaccga tcaagccgtg ctccaggcgt cggttcctgg cgtgtgtgca
    11401 cattctgggg aacactcatc gttccatcag gagtcgaaag aacggagaga ttgcccccga
    11461 tccagtagtc cacccaccgg tcttcaacct tccccatcgt gtcgtaggat tgaccgttga
    11521 tggaggcggt cgtgcttttc accaccagat catccatgcg gttgatcgcc atatcgccca
    11581 tcactcgggt atcgccccaa tgcacgaaga gaggtctctc agtctccggc gaagacacgg
    11641 acaatccttg cccggtctgc accgctgctc tcactggcgg gagatccaca gcgcccaact
    11701 ggaaccgaat tgtgctggcg gtaggcctgc tgcctcgggc tgaagccgtg acttccaccg
    11761 tgccaagtag acccggttcc accggcgcat acaatttgat catctcgaga cggcccatct
    11821 cggataaggg gccgctgaat ccacttggcg cccgattgag tatgcgatca tcgtcctcat
    11881 tgaccgcctc caagagaaga tccgggcggt caggggtgcc agtgaactgg gaacgaccgt
    11941 cggtatcaaa aaaggaggga gcgcccgttg catcggctcg ccgcttggtg agcaagtcgg
    12001 caatggacgc cggtgtcgtc gatgcgtcgt gaaaccagct gaccaccagc tcggtggcgg
    12061 cgtcagccaa ttgctgggcc tggccgtgct ctctcattgc tgatatgccc ggcgcgtcct
    12121 gcccaaccag gtacaacgcg gtcatgctca acagcgacaa catgatcacc agcatcagtg
    12181 aagccaaaag cacatgacca cgattctcaa cgagggggtg ccgtcgtatc ttcatcgtca
    12241 cctcaatcag cggttcatgt tcgcaacgtg atttctcgct tgcctgtcgc cgcacgctgt
    12301 ggagcggtga tttccacaat cacccgtcgc acaagccctg gatgcgcggt catccgacct
    12361 tgtgcatccc aatacgaaaa cctgatcgac tcgacatgtc ccaccaggac gccggcgccg
    12421 ccatcaacct gccgcaagaa tctcaagatc ccgttctcgt ccagccggct atagtagcgc
    12481 acccgattct tcaccgaagc ggaagccccg gctgggatgg gtcgaacaat cggttcgaca
    12541 aatgccaacg agctccgaag ccctgctcgg gcgagcgtga atgaatcgca gcgttcgttc
    12601 cagcagactc ccacgatctt gtggtccggc cagccacgac catcctcgac ggacagagtc
    12661 gtttctccca tcagaccagg tgcagtgacc ttcgtcatga tcccgctgat attcgccgta
    12721 aactcgacgg catcgggacg agcgaccgac agagagccga tgctcgccaa atggagttct
    12781 tgtgcgagca actcgagact cagccggacg tcttgggctt ggagaatagt atcccgctgc
    12841 atgaggaact gctggtgaaa ataggagaag gattgcaacg ccgcgcctag gacaacgagc
    12901 cccgctgcca ttgccgcgag aagttccacg agactggatc caaactggtt aataagcggc
    12961 atccttacac acgatctttt cgccgcgctc tttgtttgca tgtgcatctc cgttagtgat
    13021 gtccggctaa cacggcgttc gcgcgaagcg ttacggcccg cagttcgtga tcgccgtcat
    13081 gggaccgata gcttgcgcga gcctcgagaa ggacataccc cgagagggcg aggtttccac
    13141 cctgattcgg agccactgtc cagatcagcg tgactccgtt ctgctcgtag ctggcggaat
    13201 aaatgccgtc tccggccacg cgatctcctg cggtgccatc gtcacgcatc aatgactccg
    13261 gtaggccgtt ctgatcgaaa tcttccgtca gcaactggtc ccaacgaacc gcccgcttcg
    13321 cttcaagttt ggaatccgcc atttccatcg ctctggttgc taggaccccg cggccgaact
    13381 gttgatatgc ggcctgcaat gctcccatcg tggctaccaa tccgacggag gtcagggcca
    13441 tggccaccag cacttcagaa aacgtggcgc cggcgataga ccgacagcgc gaccagccgg
    13501 tcatgaggtc accacgcgcc cgctcaggct gacggtgatc gtggtgcgcc gattgtcccg
    13561 gtcgtggata acgatggtgc tggcggttgc cgagcgtccg ctaggatgaa agaggagttc
    13621 gcacccggct gtcggttcat cgacaacgat acccttgtcc ccatagcggt agacggtcaa
    13681 gatcgcatcg gcatccgctc gctggagtga aatcgttttc cctggaaggt cgaatcgcat
    13741 gaggaggcgc tctcgcctgg ccatggccag ttgcctcgcc attctcaagg tcgacgcgat
    13801 ttcagcagtc gccgtccttg cctgcaggcg ggcattaaga gactggaacg acggccccgc
    13861 catagaggcc atgattccga caagcgccaa cacgaccaca agttcgagga gactcctccc
    13921 tgatgacgct gaaagtcgtg tggcccctcc atgcttccat tgtctcatgg cacgctccga
    13981 tgttcgccat tcctgattcc aagacttgtg ccgcacatct cgtgtaggca tttcggtagc
    14041 attccacagc agagaccatg cagttcagtg aacttatcgc tcggcaactc ttgaaagatt
    14101 gcaatgaaaa acgcgcgagt tggatacagg caggaggtga atcagataca gaccggccag
    14161 gcacatgcgg ccgctgaacg cacagcgagg actgacaagg attggatctg ctagccggaa
    14221 ggagtcagca aggtctgata ccactcgagc agcggctggg caaaaaacat ggagacgagc
    14281 gcaccgaata cgaggaaagg gccaaagggg atgtattcat cgcgtttcat caatccaagg
    14341 ccgatcaggc tcaggcctat cagagaacca gacaatgaac cgaccataat cgtcaagagc
    14401 gccggtttcc agcccaagaa tgcgccgatc atggcgagta acttgatgtc accgccgccc
    14461 atcccctcct tgccaaacag atatgggcta gcccaggcca aggcccaaag aattcccccg
    14521 ccgatcgcta cccccaacaa ggcgttcatc aaaccaaccg gcagcaccgt ggctgcgcca
    14581 agcaggccga tgacgatgcc gggaacggtc acgacattcg gaatgattct gtgagtcagg
    14641 tcagttcctg tcaccacgag caacgccgag aacagtgccg aatacagcac cgtggtccag
    14701 gttaggccga acgttgccag aatcatcaca tatccagcag cattcacggc ctctacgacc
    14761 ggataccgca tggaaatgga gcttcggcat gttcggcaat gtcccatgag ccacaggtag
    14821 ctgaacagcg gaatgttatc gtaatacgca atggtcgtgc cgcaggaagg gcagtgagag
    14881 ccgggccaca cgatggattc gcaccggggc agccgatgaa tgcagacgtt gaggaagctg
    14941 ccgatgagcg cgccgaatcc cgctgcgagc aggtaaaaac ttccgtcaat catgacgcat
    15001 gccggtttga cttacacgta ccgacgaatc cgtacgtgca acaattgaat acgtacttat
    15061 actagggcca ttgcgcattg gtttacatga cttagaggac tttctataat ttctacagca
    15121 cgcatcacac gttctccgga agcggcgccc ctggctgcag tatagcgtaa tggatctacg
    15181 atcaaaggag aacatgaaac atggcacgta tcgcaagagt gaaatcccgg acgtctttgg
    15241 ccgatctcgc cccgccgcca aggaaaaaga gaccggagtc gttaacgagc cgggaacagg
    15301 aaattctgga gctcatatgg gctggattca agaataagga aatcggtcaa cggctcaaaa
    15361 tcagcgtaaa aaccgtggaa gcccatcgcg ccaacatgat gaagaaaatg cgcgtgtcca
    15421 ataccgccca gcttctcaag accgccattc agggcaacat tattcgggtt cgataggtcg
    15481 ctggatttgc gccggagcac ttcgctgccg cacaccttca aaaagtgtca ccgcgacggc
    15541 ggcggatacg ttcagggatg ccacctttcc tttcatcggg atgcgaacgc gaccatcgca
    15601 ttcctgcaaa acgccatgcc ggatgcctcc cccttctccg ccaaatacaa aggccactgg
    15661 ccccgtcatg tcgatggtgg tcacactgat cggggcagag ggctccacgc catacaccca
    15721 cacgccgtcc cctttcagct cttctatcag cctggataga ttgacgaccc gcgctacccg
    15781 catgtggtcc aacgctcctg ccgacacttt tgccaccacc ggcgtcaacc ccaccgcccg
    15841 gcgttcggga atgaagatgc catggacccc agccgcctcg gcggtccgca acaccgcgcc
    15901 gagattgtga ggatcttcaa ctccgtcgag gatgacaaga aacggcggct catcaaggcg
    15961 ccgggcatgc tcgagaatct catcggtgct gcagtagccc ttggccgcga tgaacgcaat
    16021 gatgccttga tgcctctggt cggggactaa gcggtcgaaa gcggctggcg gttcgatatg
    16081 caccggaaca tgttggctcc tcgcttgatg gaccagatcg gaaaattggc ggtcggtccg
    16141 cagcacgagg agacgctgga gtggacgagt tcctgctcgc aaggcttcgc ggacggcgtg
    16201 gagcccgtac agaagctccg gctgatcagc gcttccagcg ggtggtgccg tcgggcttgt
    16261 cctcgacgat gatgccctgg gccgcgaggg attttctgat ttcatcggct ttggcaaaat
    16321 cctttctttt ccgtgcttca agtcgctcac caagcaaacg ttcaatctcc gtggtcttga
    16381 gcaccgatac ttggccagca gatacggtcg cttcaacgcc ggtcaggccg actgtcttgc
    16441 cgaactgcca ctgttcagaa tccaactgaa acaatcctaa gacccctcca aattccctga
    16501 atgcagcacg gatccgctta cgactttcga ttgaatccca ccgttcaagc cgtttgttaa
    16561 cgttgcttct catattctgt agcaccgcaa gcgccatagg ggtattgaga tcgtcgtcaa
    16621 gtttctggcg ataggccaac ctggcacggt caatgatcgt tctcacttct tcgtccaagg
    16681 tagtaccctg ttggccttct tccagcctct tgaacaaatc atagaaccca tctaaggcgt
    16741 tcttcgcctc atgcaggctc tgatcggaga aatccaatgg gcttcgataa tgcgtagaca
    16801 gaaggaaata ccgcagtatt tctccgacaa tcgcttcagg ccattgttga gtctctttct
    16861 caaagatttc gcgaatcgtg aaaaaattcc ccagggactt cgacattttt tcttgattga
    16921 tctggacgaa cccattgtgg acccagtaat tggcaaattc ctttccggtc gctccgcacg
    16981 actgcgcaat ctcgttttca tggtggggga atatgaggtc catccctccg ccgtggatgt
    17041 cgaacgtctc gcccagatgt ttgatcgaca tggctgagca ctcaatatgc catccgggac
    17101 gccccggccc ccaggggcta tccca
//
LOCUS       DASSCQ010000117         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_848482, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000117 DASSCQ010000000
VERSION     DASSCQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848482"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /gene="pelF"
                     /locus_tag="VFH05_08165"
     CDS             <1..1280
                     /gene="pelF"
                     /locus_tag="VFH05_08165"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HET6678228.1"
                     /translation="EYMSRTAEPASVTVSTPSVLLNPSHTQPRPAAVGSADRIRVMKF
                     VGIFAIGGTERQVVNIGKGLDKSRFDLRFATLNKVGELLAECDAQSWAIDEYKIRRLY
                     GLATMRKQIEFGKTLRRARTQILHAYGIYPNVFAIPAARLAGVPVTIGSIRDIGDTYT
                     YWQHQVQKHCLRMADHIVVNAEAIKRDLLQRGYDAKRLSVIPNGIDCERFRQPGNGEA
                     VRREWNIPPGVPVVGVLARLLRIKGQDVFLRAAALIASTNPQVRFVIVGDNNIDQQYK
                     EELKELTRRLGLEDRVIFTGFRMDVPDLLAALSIVVSPSLGLEGLSNSLLESMATGLP
                     VVATRVGGTPEIVEDGESGLLVPPGDPSALAAAISRLLQDRMTAKRLGQSARRQVLSR
                     YSLEQAVASTERLYDDLLLQARHKGRVKERARS"
     gene            1277..2935
                     /locus_tag="VFH05_08170"
     CDS             1277..2935
                     /locus_tag="VFH05_08170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6678229.1"
                     /translation="MNALALYRFGHWCYRRRIPLLPALVYRLIYVLHHAVIPMSVEIG
                     EGSELAYGGLGTVLHERCKIGKFVTIGHQVSIGGRSRRWGVPIIEDRCVIGAGAILLG
                     PISIGAESVVGANAVVLEDVPPKTVVAGAPATVVRKDIDIMDYTCLQPPEEAEALSFR
                     STDNLLVTVIQDSGRVRHLADEWTELLSDSDADCVFLTREWLETWWEHFSPKRTLALI
                     TVRRQNRLLGLAPCFMETKRFGGIMPYPSLQFLGTGLVGSDYLDVVIRRGHEAQVSRS
                     LAEYFQSRRLVMTLSHVKQQGALAAGFVKELEQAGWTVERRPIDACPHIPLKGHTWES
                     YLASLGSQHRYNFNRRLKNFQKEGTVVFDVVEKEDQRREALETLIDLHNLRWDERGGS
                     ETWQSPAMRAFHEAFTRTALERGWLRLFILRLNGCPVGALYGLLYGRRFYFYQSGFDP
                     MYRQHSVGLLTMGLAIKHALAEGAEDYDLLHGKETYKFLWAKETAELDRLQLYHPGVG
                     GAVCRRAVMLDELAKTAARRYLPPAVLEHAAAWRRGEILRSSSC"
     gene            2929..4062
                     /locus_tag="VFH05_08175"
     CDS             2929..4062
                     /locus_tag="VFH05_08175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6678230.1"
                     /translation="MLKAFAKTGVAAVLGWTGADRAVSSLAGTMRLPLVVGYHAVVED
                     VRAHVGRAIAPNLISVNMLERQLEWIGRRYRFMTLDDLGAQLERGDPFERPSAAVTFD
                     DGYVGVYRHALPLLKRMGIPAGVFIVTELTGRSELQLYDKIYLLLEKALPLLGHSEMR
                     LRQLLQTKNVSLGERHGARRLEAFSTMRWLLNTQTQKGLRRVIEALETVSGIRDSDYP
                     DLQVMDWEMVRTMDASGITIGSHTQTHALLTEESLERAANQAVGSMYTLRDRLQRPIR
                     HFAYPDGRFNAAVVSAVARAGYRYGYGTCLRRDHRHPNLTIPRTLLWERSCIDARGRF
                     SSSLMRCHAYRLTDLWAPCTHNHGDSATSAASQRSEARQRQHA"
     gene            4055..5536
                     /locus_tag="VFH05_08180"
     CDS             4055..5536
                     /locus_tag="VFH05_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET6678231.1"
                     /translation="MLKPALTLMAGRTLGYAVLFLVPIILVRLFSQEEFGTYKQVFLL
                     YGTILNLAQVGMSESLFYFIPGSSEDAGRYVCNSILVLGGIGLLTGGLLYLGGDLIAH
                     SLNNSALSPLMPLLAAYFFLMLASYVLEIVMTARHQYSYAAAAYGLSDVARAALIVMP
                     ALVFQTVASVLYGAIVHAVIRLGTTIWYSVKQFGAGLRPDRSVLLRQLAYSLPFALYV
                     LFQTGQESLHLFVVSSWFDATTFAIYSVGCLQIPLAEVVSMSVVNVMMVGMVQAIRDG
                     REAAVVPMWHDTVRKLALMFFPFAAILFITARDLIIFLFTDAYAASVPIFRTWSLVVL
                     LSAVPMDGLLRVYAQTPFLLVINVVKFILVAGAIYWFVSGLGLIGPVVITLLALAAGK
                     AIGLARMMVCGHLNGGNVLPWRSLLEIGFVAGAASLPAWWITAQVSAPPVARLAMTAV
                     TYGVVYVGLALLCGVIRKNEQDRVLRWIQPAWRPMRLKALFMR"
     gene            5533..>6187
                     /locus_tag="VFH05_08185"
     CDS             5533..>6187
                     /locus_tag="VFH05_08185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6678232.1"
                     /translation="MRKPTLPHITIVAPHVTKSGGGQALHAADLSDHLRRDGYSVHVL
                     PIDPDFPPGARWVRRVPYLRTLLNQSLYLPSLLTLRRTDIVHVSSASYWSFLLAPLPA
                     MLIGRLLGKPVILNYHSGEAADHLGRWGVLVHPWLSLADEIVVPSEYLRQVFKSHGYC
                     TRVIHNTVDTSRFMYRVRDPLRPRLLSTRNFEWYYGVDHTLRAFKLIQRTYPDATLEI
                     "
BASE COUNT         1142 a         1775 c         1866 g         1404 t
ORIGIN      
        1 aggaatacat gtctcgaaca gctgaacccg catccgtgac tgtctctacg ccgagcgtcc
       61 tgctcaatcc gagccacact cagcctcggc cggcggctgt cggatccgca gatcgcatac
      121 gggtcatgaa gttcgtcggc atctttgcca ttggcggtac cgaacgtcag gtcgtgaaca
      181 tcggaaaggg tttggacaag tcacgcttcg acttacgttt cgccaccttg aataaggtcg
      241 gtgagcttct cgccgaatgc gatgcgcaat cctgggcaat tgacgaatac aaaatccgtc
      301 gactctatgg cttggcaacg atgaggaaac agatcgagtt tggaaagacc ttgcgtcggg
      361 caagaacaca gatcctgcat gcgtacggca tctacccgaa cgtgtttgcc attccagccg
      421 cgcgactcgc aggcgtacct gtcacgatcg ggtcaatccg tgacatcggc gatacctaca
      481 cgtactggca gcatcaagtc caaaaacact gtctccgcat ggccgatcat attgtggtga
      541 atgcggaggc aatcaagcgg gatttgttac agcgcggata cgatgccaag cgcctgagcg
      601 tcataccgaa cggcatcgac tgcgagcgtt tccgtcagcc gggtaacggc gaggcggtgc
      661 gtcgggaatg gaacattcct cccggggtac ccgttgtcgg cgtcttggcc aggctgctgc
      721 ggatcaaggg acaggacgtg tttctccggg ccgccgcatt gatcgcatcg accaacccgc
      781 aggtccggtt cgtgatcgtg ggagacaata atatcgacca gcagtacaaa gaagagctca
      841 aagagttgac gcgccggctg ggcctggaag atcgcgtcat attcaccggc tttcgcatgg
      901 atgttccgga tctgctggcg gcactatcca tcgtcgtgtc accgtcgctc ggcttggaag
      961 gactgtccaa ttcgctcttg gagtccatgg caactggtct tccagtggtg gcgacacggg
     1021 tggggggcac gcctgagatc gtcgaagatg gggagagcgg cctcttggtt ccacccggag
     1081 atccgagtgc cttggccgca gcaatctcca gactgctgca agacaggatg acggcgaagc
     1141 gcctcgggca atccgccaga cgtcaagtct tgagccggta ttcgctggaa caagccgtcg
     1201 cctccaccga acggttgtat gacgacctct tgctgcaggc acgacacaag gggcgcgtca
     1261 aagaacgggc acgttcatga atgcgttggc attatatcgg ttcgggcact ggtgctaccg
     1321 gcggcgaata cccctcctgc cggcgttggt gtatcgcttg atctatgtgc ttcatcacgc
     1381 cgtcattccg atgtcggtgg agatcggcga gggatcggag ttggcgtacg gcggactcgg
     1441 aacggtcttg catgaacgct gcaagatcgg caagttcgtg accattggcc atcaggtgtc
     1501 gattggcggg cgttcgcgcc gatggggcgt gccgatcatt gaggaccgtt gcgtgatcgg
     1561 tgcgggagcg atcttactgg ggccgatttc gatcggcgca gaatcggtcg tcggagcgaa
     1621 cgccgtcgtg ttggaggacg taccgccgaa gacggtggtg gccggcgctc ctgccaccgt
     1681 cgtccgaaag gacatcgaca tcatggatta cacgtgcttg caacctcctg aggaagctga
     1741 agccctgtcc tttcgatcga ccgataatct gctcgtcacg gtcattcaag attccggtcg
     1801 cgtccgtcat ctggccgacg aatggacgga actactcagt gacagtgatg ccgattgcgt
     1861 gtttctgacg cgggaatggc ttgagacctg gtgggaacat ttctccccga aacggacatt
     1921 ggccttgatc acggtgcggc gtcagaaccg cttgctcggc ctggctccct gcttcatgga
     1981 gaccaaacgg ttcggcggca tcatgcccta tccgtcactc cagttcctgg gaacgggact
     2041 cgtggggtcc gattatctgg acgtggtcat tcgacgggga cacgaagcac aagtgtcccg
     2101 cagcctggcg gaatactttc aaagccgccg gctggtgatg accctcagtc atgtgaagca
     2161 gcagggcgct cttgccgccg gattcgtcaa agaattggaa caagccggat ggactgtgga
     2221 gcgccgtccg atcgacgcct gcccgcatat tcctttgaag ggacacacct gggaatccta
     2281 tctcgccagc ttgggatcgc agcaccggta caatttcaat cgcagactca agaattttca
     2341 gaaggagggc acggttgtct tcgacgtggt ggaaaaggaa gaccagcgcc gtgaagcgct
     2401 tgagacgttg atcgacctgc acaatctccg atgggatgag cgcggagggt ccgaaacctg
     2461 gcaatcgccg gcgatgcgtg cctttcatga ggccttcacc cgcacggcat tggagcgcgg
     2521 atggttgcgg ctcttcatcc tccggctgaa tggctgtccc gtcggtgcct tgtacggatt
     2581 gctctatggt cgtcggttct atttttacca gtcaggattc gacccgatgt atcggcagca
     2641 cagcgtggga ttgctcacga tgggtctcgc catcaagcac gcgcttgcgg aaggtgcaga
     2701 ggactacgat cttctccatg gcaaggaaac gtacaagttt ctgtgggcga aggaaaccgc
     2761 cgaacttgat cggctgcagc tctatcaccc cggtgtagga ggagcggtgt gccggcgagc
     2821 cgtcatgctc gatgagctgg ccaaaaccgc cgcccgccgg tatctaccgc cggcggttct
     2881 cgaacacgcc gccgcctgga ggcgcggaga aatattgcgg tcctcctcat gttaaaagcc
     2941 tttgccaaga cgggtgtcgc tgcagtgctg ggatggaccg gggctgatcg cgcggtcagc
     3001 tcgctggcag ggacgatgag gcttccgctg gtggtgggct atcatgcggt cgtcgaggat
     3061 gtccgcgcgc acgtcggacg cgcgattgcc cccaatctga tcagcgtcaa catgctcgaa
     3121 cgccagttgg agtggatcgg tcgacgatat cgcttcatga cgctggacga tctcggggcc
     3181 caattggaac gaggagatcc gttcgagcgg ccgtccgccg ccgtgacgtt cgacgacggc
     3241 tatgtcggtg tctatcgtca tgccctgccg ctgttgaagc gaatgggcat tcctgccggc
     3301 gtcttcatcg tgacggagtt gacgggccgt tcggaactcc agttgtacga caagatctat
     3361 ttgctgttgg aaaaggcgct tccgcttctc gggcattccg agatgcgatt acggcaactc
     3421 ctgcaaacca agaacgtgtc cctcggcgag cgccacggag cgcggcgact ggaggccttc
     3481 agcacgatgc ggtggctgtt gaatacccag acgcagaaag gtcttcgccg cgtcatcgag
     3541 gcgttagaaa cggtgtcggg aatccgggac tcagactatc ctgacctgca ggtgatggat
     3601 tgggagatgg tgcgaacgat ggacgcgtca gggatcacga ttggctccca cacgcaaacc
     3661 catgcgctgt tgacagaaga gagcctcgag cgagccgcca accaggcggt gggatccatg
     3721 tatacattgc gggatcggct tcagcgcccg atcaggcact ttgcctatcc cgacgggcgc
     3781 ttcaatgccg ccgtcgtgtc tgccgttgcc cgggccggat atcgctacgg ctacgggacg
     3841 tgtctgcgtc gtgatcaccg acaccccaat ctgaccatac cgcgtacgtt actgtgggaa
     3901 cgatcctgca tcgatgctcg tgggagattt tcttcttcgc tcatgcgttg tcatgcctac
     3961 cgcctgaccg atctctgggc tccttgcacg cacaatcatg gcgattccgc aacctcggca
     4021 gcttcccaac ggtccgaggc ccggcagagg cagcatgctt aagccggcgc tgactctcat
     4081 ggcaggccgg acgctggggt atgccgtctt gtttctcgtt ccgatcattc ttgtccgtct
     4141 gttcagccag gaggagttcg gcacgtacaa acaagtgttc ctgctctatg gcacgatcct
     4201 caatctggcg caggtcggga tgtcggagag tctcttttat ttcattccag gctcatcaga
     4261 ggacgctggc cgttatgtct gtaactcgat cctggttctc ggtggcatcg gtctgctgac
     4321 gggaggcctg ttgtatctcg gcggggacct gatcgcgcat tccctgaaca attcagctct
     4381 ttcgccgctg atgccattac tggcggccta cttctttctg atgctggcgt cgtatgtgtt
     4441 ggagattgtc atgacggctc gccaccagta ttcctatgca gcggcggctt atggattatc
     4501 ggatgtcgct cgcgcggcct tgatcgtcat gccggcgctg gtgttccaga cagtggccag
     4561 cgtgctctat ggggccatcg tgcatgccgt gatccggctc ggcacgacca tctggtatag
     4621 cgtcaagcag ttcggcgccg gcctccgtcc cgaccggtcc gtccttctgc ggcaactggc
     4681 ctacagcctg ccgttcgcgt tgtatgtcct atttcaaacc ggccaggagt ctttgcatct
     4741 ctttgtggtg tcctcctggt ttgacgccac cactttcgcc atctactccg tcggatgcct
     4801 ccagattcct ctggcggagg tggtcagcat gtcggtggtc aacgtcatga tggtcggcat
     4861 ggtgcaggct attcgggatg gtcgtgaggc cgccgtcgtt ccgatgtggc acgacaccgt
     4921 gagaaagctg gccctgatgt tttttccatt tgcggcgata ctgttcatca ccgctcgcga
     4981 tctcatcatt tttcttttta ctgacgccta cgcggccagc gttccgattt tccgaacctg
     5041 gtctctcgtc gtcctcttgt cggccgtgcc gatggacgga ttactcagag tctatgcgca
     5101 gaccccattt ttattggtca ttaacgtcgt caaattcatt ctcgtggccg gggcaattta
     5161 ctggttcgtg tctggcttgg ggttgatcgg acctgtcgtc atcaccctgc tcgctttggc
     5221 agcggggaag gccattggct tggcacgcat gatggtatgc gggcatctga atggagggaa
     5281 tgtcctgccg tggcgcagcc tgctggaaat cggctttgta gcgggcgccg cgtcacttcc
     5341 tgcctggtgg atcaccgcac aggtttccgc tcctcctgtg gcgcggctcg cgatgaccgc
     5401 cgtcacgtac ggagtggtct acgttggtct tgcgctgctc tgtggggtca ttcgaaaaaa
     5461 cgaacaggat cgggtcttgc gctggattca gccggcgtgg cgccccatgc gcctcaaagc
     5521 gctctttatg agatgagaaa gcctacgttg ccgcacatta ccattgtcgc tccccatgtg
     5581 acgaagtccg gcggcggcca agcgctgcat gcggcggact tgtccgacca tttgcgacga
     5641 gacgggtatt cggtacacgt gctgccgatc gatccggatt ttccgcccgg cgcccgttgg
     5701 gtacgacggg ttccgtatct tcgcaccctt ctcaatcaga gtctgtatct ccccagcctg
     5761 ctgacattgc gccggaccga catcgtccat gtgtcatcag cctcctactg gtcgttttta
     5821 cttgctccac ttccggcgat gctcatcgga cggctcttgg gcaaaccggt catcctgaac
     5881 tatcacagcg gggaggccgc cgaccatctg ggccggtggg gagtactggt tcatccgtgg
     5941 ttgtcgctgg cggacgaaat cgtcgtccct tccgagtact tgcgtcaggt attcaagtcc
     6001 cacggctatt gcacgagggt cattcataat accgtcgata cgagccgatt catgtacagg
     6061 gtacgcgacc ccctccggcc gcggttgctc tccacacgga atttcgaatg gtactacggg
     6121 gtggatcaca cgttgcgcgc attcaagctt atccagcgta cctatcctga cgccacgctc
     6181 gagatcg
//
LOCUS       DASSCQ010000118         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1345478, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000118 DASSCQ010000000
VERSION     DASSCQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345478"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..2002)
                     /locus_tag="VFH05_08190"
     CDS             complement(173..2002)
                     /locus_tag="VFH05_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HET6678233.1"
                     /translation="MTRSSSTPPVIDHPLNVIVATDCGSTTTKAILIEKVGDEYRQTY
                     RGEAPTTVEAPFEDVTRGVLNAIAEIEELSKRKILDGDKIITPCRDARTGVDIYISTS
                     SAGGGLQMMVTGVVQNMTGESAQRAALGAGAIVIDVLASNDGRLPHEKIERIRSMRPD
                     MILMSGGTDGGAVTHVAEMAEYIAAAEPRPRFGVTYKLPLIYAGNTDAQPQVRRILGE
                     KTALEVTENIRPVLERENLAPARNKIHDLFLEHVMQQAPGYKKLIEMTGAPIMPTPAA
                     VGLIMETIAKREHLNLIGVDIGGATTDVFSVFDGVFNRTVSANLGMSYSISNVLAEAG
                     LANIMRWVPFTIDEQTLRNRIKNKMIRPTTIPQTLDELQIEHAISREALRLALIHHKS
                     LATGLKGIQQERTISDVFEQQTSGRTLIDMLKLNLIVGSGGILSHAPRRIQSMLMMVD
                     AYEPLGCTMLSVDSIFMMPHLGVLSTINEQAATDVFVRDCMIYLGTCIAPIGQGKERD
                     LCADYEIVFPDGRLVKDQLAFGELRLFALEPGMKANITLRPAKSVNLGTGAGAAMTQE
                     VMGGAVGLLLDGRGRPLQLPAEQSARVAALKKWFNAVGLYPQG"
     gene            complement(1999..2268)
                     /locus_tag="VFH05_08195"
     CDS             complement(1999..2268)
                     /locus_tag="VFH05_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678234.1"
                     /translation="MDDVALMERIADVLVRRGMSAPATMFLDSMGPMNFLGSQALHFL
                     TPIIDCAFNAKEVEQVARLLERRETISQLIAVIEARSSGQKASAQ"
     gene            complement(2523..3125)
                     /gene="plsY"
                     /locus_tag="VFH05_08200"
     CDS             complement(2523..3125)
                     /gene="plsY"
                     /locus_tag="VFH05_08200"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET6678235.1"
                     /translation="MQEEPVCILLSMFGFLLGSIPFGIVVSKALNLSDPRTVGSKNVG
                     FTNVLRVSGTKAGVLTLLGDMGKGWVMGWAAMQWLTIESFIMVVAVSPILGHLFSPFL
                     GFKGGKGVATALGAVLGLSPSIGILLLLIWIGAVAIWRYSSGGALAAFGLFPVVAIVN
                     EQRQEFLLFAIFVSALVWVKHKDNIVRLWKGTEGKIGQRA"
     gene            complement(3135..3731)
                     /gene="pgsA"
                     /locus_tag="VFH05_08205"
     CDS             complement(3135..3731)
                     /gene="pgsA"
                     /locus_tag="VFH05_08205"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET6678236.1"
                     /translation="MNRVLEAWKSVGQESLNLPNFITLVRILLIPVFVMLFFTPTPDR
                     SLSAAFVFVVAAITDLLDGYIARRTGQVTKLGKLLDPIADKLLVLSALILLVNIDRVS
                     ALVAILVIAREVAVTGVRAIAAGEGMIIVAETTGKYKMALQVVATTMLILEGTVLAEF
                     GNLHLAGSVTLYLSLVLGYVSGGLYVWSFWKQVVAKGL"
BASE COUNT          898 a         1200 c         1096 g          820 t
ORIGIN      
        1 tctcacgacc gttctatcgg cgacagcaag tcctggagta tacgaatgtg ccatagtcgc
       61 tagaacatcc ctccgccttc aaaaatattt cttaccccgc ttggaatcga acccagcggt
      121 tcacgagaca gtgtcttgag cttctgaacc tcatggcccc gtggcatcat ttctaaccct
      181 gaggatataa accgactgcg ttgaaccatt ttttcagggc agcgacccgt gcagactgct
      241 cggctggtaa ttgcaatgga cggcctcgac cgtcgagtaa taagcccacc gcaccgccca
      301 tgacctcttg cgtcatggcg gccccggctc ccgtcccgag attgaccgat ttcgccgggc
      361 gtagcgtgat gttggctttc atgccgggct ccagcgcaaa cagcctgagt tcgccaaacg
      421 ccaattggtc tttcacaagt cgaccatcgg gaaacacgat ctcgtagtcg gcgcagaggt
      481 cgcgttcctt cccctgtcca attggtgcga tgcacgtgcc gagataaatc atgcagtccc
      541 ggacaaacac atcggtcgca gcttgttcat tgatcgtcga gagcaccccc aagtgcggca
      601 tcataaaaat gctgtcgacc gacagcatgg tacagcccaa gggctcgtag gcatcgacca
      661 tcatgagcat cgactgaatc cggcgtggcg cgtgagaaag aattccgccg cttccgacga
      721 tcagattcaa tttcagcatg tcgatcagcg tgcgtcccga cgtctgctgc tcgaatacgt
      781 ccgaaatcgt gcgttcttgc tgaatccctt tcaatccggt ggccagcgat ttgtgatgga
      841 tcaaggccaa ccgcagcgct tcccgggaaa tcgcatgctc gatctgcagc tcgtcgagcg
      901 tctggggaat cgtcgtgggc cggatcatct tattcttgat gcgattgcgc agtgtctgtt
      961 catcgatggt gaacggcacc caacgcatga tgttcgccag gccggcctcg gccagcacat
     1021 tggaaatgct gtaggacata ccgaggtttg cgctcaccgt ccggttgaac accccgtcga
     1081 acaccgaaaa gacgtcggtg gttgcgcccc caatgtcgac gccgatgaga ttcaagtgtt
     1141 ctcgcttggc gatcgtttcc atgataagcc cgacagccgc gggagtcggc atgatcggtg
     1201 cgccggtcat ctcaatgagt ttcttgtaac cgggcgcctg ctgcatcaca tgctcgagaa
     1261 acaagtcatg gattttgttc cgggccggag ccaagttttc tcgctccaag actggacgga
     1321 tgttctccgt cacttccagg gccgttttct cgcccagaat cctcctcacc tgtggctggg
     1381 cgtctgtatt cccggcatag atcaggggca acttgtaggt gacgccgaag cggggacgcg
     1441 gctcggccgc ggcaatgtat tccgccattt cggcgacatg cgtcacggca ccgccgtccg
     1501 tcccgccgga catcaaaatc atatcgggcc gcatcgaacg aatccgctcg atcttctcat
     1561 gcggcaaacg gccgtcgttc gacgccagta cgtcgatcac gatggcgccg gcaccgaggg
     1621 ccgcacgttg cgcgctttct ccggtcatgt tctggaccac gccggtcacc atcatttgca
     1681 acccgccgcc ggcgctgctg gtcgagatgt agatgtccac gccggtgcgt gcatcccggc
     1741 agggcgtgat gatcttatcg ccgtccagaa tcttgcgttt ggacagttct tcaatttccg
     1801 caatcgcgtt gagcacacct cgagtgacgt cctcaaaagg cgcttcgacc gtcgtcggtg
     1861 cctcgccgcg gtacgtttga cggtattcgt cgccgacctt ttcaatcagg atggccttcg
     1921 tcgtggtgct gccgcaatca gtcgccacga tgacgttgag aggatgatca atgacgggcg
     1981 gggtcgagga agaacgcgtc attgagccga tgccttttgc cctgacgacc tggcctcgat
     2041 gacggcgata agctgcgaga tcgtttcacg acgctcgagg agacgcgcaa cctgttcgac
     2101 ttccttggca ttgaatgcgc aatcgatgat gggagtgaga aaatgaagcg cctgactgcc
     2161 caaaaagttc atcggtccca tggagtctaa aaacatcgtg gcaggagccg acatcccgcg
     2221 ccgtacaagc acgtcggcga tgcgctccat cagagcgaca tcatccatgc tgagcggccg
     2281 actgtcggag tgagttgaaa aagcatgtcg gagacctgtg cgcaagtcgc gcagtctctt
     2341 ggtgagatga tccttgaaca aggggcgcat agggttgata ggacaagaga aacggaggtc
     2401 aacgcattat atggacggct accgggagag tcaatttgag ggacggcgga atggcttcgc
     2461 aagagcgcca acgcgacact ttgtggtcac ggtaggcagg ttcgttctgc tcgacggcgg
     2521 gattacgcgc gttggccaat ttttccttcc gtccctttcc agaggcgtac gatgttgtcc
     2581 ttgtgtttga cccagaccag cgcgctgacg aaaatggcga aaagcagaaa ctcttgccgc
     2641 tgctcgttga caatggcgac caccggaaag aggccgaagg cagcgagcgc acctccggat
     2701 gaataccgcc agatcgcgac cgctccgatc cagatcagca gcagcaagat gccgatcgaa
     2761 ggtgaaagcc ccagcaccgc ccccaaggcc gtcgcgaccc ccttgccgcc tttgaagcca
     2821 agaaatggag agaaaagatg gccgagaatc ggcgacacag cgactaccat gatgaaggac
     2881 tcgatcgtca gccactgcat cgccgcccag cccatgaccc acccttttcc catgtcaccc
     2941 agaagcgtca gcacgccggc cttagtaccg gacactcgca ggacgttcgt gaatcccacg
     3001 ttcttgctgc caacggtgcg tggatcagaa agattgagag ctttggagac aacaatcccg
     3061 aaaggaatcg aacccaggag gaatccaaac atcgacagca ggatacagac gggttcttct
     3121 tgcatcgata ctcttcacag acctttggcc accacttgct tccagaaact ccacacatac
     3181 aagccgcccg acacgtaccc gagcacgagc gacagataca gcgtcacact gccggctaaa
     3241 tgaagattgc cgaattcggc gaggacagtg ccctcgagga tcaacatcgt ggtggcgacc
     3301 acctgcagag ccatcttgta tttgccggtc gtctcggcca cgatgatcat gccttccccc
     3361 gccgcgatcg cgcggactcc ggtgacggcc acttctctcg ctatcaccag gatggccacc
     3421 agcgcactga cgcgatcgat gttgacgagc aagatcaatg cggacaacac caacagcttg
     3481 tcggcgatcg ggtcaagcag cttgccgagt ttcgtgacct ggcccgtgcg acgggcgatg
     3541 tacccgtcca acagatccgt gatcgccgcc accacgaaga cgaacgccgc gctcaacgaa
     3601 cggtccggcg tgggggtaaa aaacagcatc acgaacacag gaatcagcaa aatacgcacg
     3661 agcgtgatga aattgggcaa attcaaggac tcttgcccga ccgacttcca agcttcaagg
     3721 acacgattca tgacgatgta gttgctccct cttttcgata gaatgaactc gttcacacta
     3781 aagcgggtat atttctcacc cgcccaccct cgcggcgccg agacgcggcg tctgcccaca
     3841 tctcacccac ctacctggtg agttgcagat catatctccg agcacgcgaa tacgatggag
     3901 ggcactgttg catggttcag ctacacgatg ccgttcttga gcaaatcatg caggtgaatc
     3961 acgccgaaca tcttccgatc gctctcggca accaccaagg tggtaatgga aaac
//
LOCUS       DASSCQ010000119         6361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1784579, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000119 DASSCQ010000000
VERSION     DASSCQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784579"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1421
                     /locus_tag="VFH05_08210"
     CDS             198..1421
                     /locus_tag="VFH05_08210"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET6678237.1"
                     /translation="MRILVINSGSSSIKFRLAEVVEENGTLAVRTPLVEGAVKGIGGV
                     ASFEMRGSRADRSTSMRNMADHTQAMRVLFDRLGDSLSGIDAVGHRVVHGGSQYLEPT
                     VITDEVEAGIDALSELAPLHNPGCLAGIRGAKAVLQSPVPMVAVFDTAFHQTMPEVAR
                     SYAIPTDMAERHRIRRYGFHGIAHASLANGYAAFTGKSLKLCSLITVQLGNGCSVAAI
                     ANGKSVDTSMGFTPLEGLVMGTRSGDVDPSLVSYLCEREKVEPAEVERWLNQRSGLLG
                     VSGRSNDMRELLRAAQQEQDQRAQFAIDLFCYRVRKYVGGYLAVLGGAEAVVFGGGIG
                     ENAPEVRRRICRNMEWCGVRLHPDRNEVAVGLYSGSAAKISADDAGLAVYVVAADEEA
                     WIAKETARCVRATTS"
     gene            1471..3882
                     /locus_tag="VFH05_08215"
     CDS             1471..3882
                     /locus_tag="VFH05_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET6678238.1"
                     /translation="MMGNALSEKELKLIDAYWRAANYLSVGQIYLYDNPLLRQSLKKE
                     HVKPRLLGHWGTTPGLNFIYAHLNRVIKQRDLNMIYIIGPGHGGPGIVANAYMEGTYS
                     EVYPNISQDEEGMKRLFKQFSFPGGIPSHVAPETPGSIHEGGELGYAVSHAYGAAYDN
                     PDLIVACVVGDGEAETGPLATSWHSNKFLNPLADGVVLPILHLNGYKIANPCVLARIS
                     HQELDQLFRGYGYTPYFVEGSDPEKMHQTMAATLDTIIEDIHRIKTEARRTGAKKRPL
                     WPMIILRSPKGWTCPKEIDGKRTEDYWRSHQVPMGEMHEKAEHVKILEKWMKSYKPED
                     LFDKAGRLKSELAELSPKGERRMSANPHANGGLLLRDLRLPDFQDYAAEVTKPGATMA
                     ESTRHMGKFLRDIMKLNMDSRNFRLFSPDENNSNRWQDVLDVTNRAWMAESYPYDDHL
                     APDGRVMEMLSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIIDSMFNQHAKWLKVCNEI
                     PWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKADVVRVYLPPDANTLLSV
                     TDHCLRSRNYINVIVAGKQPAPQWLTMDQAIKHCSAGIGIWEWASNDKEGDPDVVMAC
                     CGDVPTLETLAAVDIIRQHVPALRIRVVNVVNLMKLQPPSEHPHGLSDFDFDALFTKD
                     KPIVFAFHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTTSTPFDMVVMNDLDRFHLVAD
                     VVDRLPQLGSRAAYAKQAIQEKLLEHKQYIARYGEDMPEITDWQWGQASGKSKRKSST
                     EGDNV"
     gene            3915..>6361
                     /locus_tag="VFH05_08220"
     CDS             3915..>6361
                     /locus_tag="VFH05_08220"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET6678239.1"
                     /translation="MTQEGLKDLLQQYGVGPIQFSGTHDALYERHLTFDHVVDVKRAD
                     VREQFEAAARSIRDIISQRWLRTERTYAEKNPKRVYYLSMEFLLGRSFANNVTNALLR
                     PLATEAAKTKGLDPFALIEAEPDAGLGNGGLGRLAACFIDSMATMGIPGMGYGLRYEY
                     GIFKQTIVDGWQCEQPDHWLRRPDPWEVARLEEAVEVKLNCSFELRGGMLHPVIGRPS
                     TLLGIPFDRPVVGYGGKTINTLRLWAASTPDYFDFQQFSRGDFVGALAETLTAETLTR
                     VLYPDDSTSMGQGLRFLQEYFLVACSLGDLVRRFRRSNTQWQALPDKVAAQMNDTHPA
                     MAVAELMRILLDEAHLEWEEAWDLTQRTLAYTNHTLLPEALEKWPLEWFEAMLPRHLE
                     IILEINRRLIEGVRHRFPGDDGRIERVSLVEEGARRKIRMANLAIVGSHSTNGVAAIH
                     SRLLRTMTVKDLAELYPERFNNKTNGVTPRRWLLLANPGLANAITEAIGDRWITDLSQ
                     LTKLKALAGDPAFRDSFLRAKREAKAQFAQWLETTSGQHINPDSMFDCQVKRIHEYKR
                     QLLNALRIVVLYNRLRENPDQPMAPRTFFFAGKAAPAYHLAKVIIKFINNLAGTIDGD
                     PLVRGRIKVVFLPEYCVSLAERLIPATDASNQISTAGYEASGTSNMKFMMNGALTIGT
                     RDGATIEMAEEAGEENFFLFGLTAEQVAASRNWYNPRWHYDHEPETRAALDLIFSNYF
                     SRNEPGTFAALEQALLTGGDHYMHLADLSSYVNADRELCHLYSDSKQWAHKAILNIAG
                     SGKFSSDRTIAEYAAGI"
BASE COUNT         1420 a         1743 c         1926 g         1272 t
ORIGIN      
        1 gtcgaaatca cggcctcatc gccagttttt cacgggcgct ggtcgaaggg ctgtcagccc
       61 agcaatccga tgctgagttc aatgccatgc tggataagtc cattcagagc atctttgacg
      121 cgtccaatac ttaagacaaa cggggttgac cgctttgact cgcatgatga ctgaaggcat
      181 gaggtgtcag cgttgacatg cggattctgg tgatcaatag cggcagctcg tcaatcaagt
      241 ttcggctcgc agaggtggtt gaggaaaacg ggacattggc ggttcgaacc cccttggtcg
      301 aaggggccgt caaaggcatc ggaggtgtgg cgagctttga gatgcgagga tcccgcgcgg
      361 accgatctac gtcgatgagg aatatggccg accacaccca ggcaatgcgg gtgttattcg
      421 atcggctggg cgattcgctg agtggaatcg acgctgttgg gcaccgggta gtgcacggcg
      481 ggagtcagta cctcgaaccc accgtcatca cagacgaggt cgaagcagga atcgatgcgc
      541 tatcggaatt agcaccgttg cacaatcccg gatgtcttgc gggaatccga ggagccaaag
      601 cggtcctgca atcacctgtc ccgatggtgg ccgtgtttga taccgccttt caccagacga
      661 tgccagaggt tgccagaagt tatgcgattc ctactgatat ggccgaacgc caccgcatcc
      721 gccgttacgg ctttcacgga atcgcccatg cctcgctggc gaatggctat gccgccttca
      781 cagggaaatc cttgaaactg tgcagcctca ttacggtgca attgggcaat ggctgctcgg
      841 tcgctgcgat cgctaacggg aagtccgtcg acacctcgat gggattcacg ccgctggaag
      901 gactggtcat gggaacacgc tcaggagacg tcgacccttc cctcgtcagt tatctctgtg
      961 agcgtgaaaa agtcgagccg gccgaagtgg aacgatggct caatcagcga tccggcctcc
     1021 tgggagtctc aggacgatcc aatgacatga gagagctgct gcgcgcagcg caacaggaac
     1081 aggaccagcg tgctcagttt gccatcgatc tattttgcta tcgcgtgcgc aagtatgttg
     1141 gaggctacct cgccgtgttg ggcggtgcgg aggcggtcgt cttcggggga ggaatcgggg
     1201 aaaacgcacc ggaggtgcgg aggcgcatct gccggaacat ggaatggtgc ggggtgcgcc
     1261 tccatcccga ccgcaacgag gtcgccgtgg gcctctacag cggatcggcg gcgaagatca
     1321 gtgcggacga cgccgggctg gccgtgtatg ttgtggcggc ggatgaggaa gcatggattg
     1381 cgaaagaaac ggctcgctgt gtgcgggcga cgacgtcata agtcgttcag tatagggcat
     1441 gaatggccgg ggcgaacgag gaaggagtaa atgatgggca atgcactatc ggaaaaagag
     1501 ctgaagttga tcgatgccta ttggcgggca gcgaattatc tctcggtcgg gcagatctat
     1561 ttgtatgaca atccgctgtt gagacagtca ctcaagaagg agcacgtcaa acctcgctta
     1621 ctcggccact ggggcacgac gcccggcctc aattttatct atgcgcactt gaatcgggtc
     1681 atcaagcaac gcgacctgaa catgatctac atcatcggcc cggggcacgg cggtcccggc
     1741 atcgtcgcca atgcctatat ggaagggacc tactcggagg tgtacccgaa catctcacag
     1801 gatgaagaag gcatgaagcg cttgttcaag caattttcct ttccgggcgg catcccgagc
     1861 cacgtggctc cggaaacgcc cggatcgatc cacgaggggg gcgaattagg gtacgccgtg
     1921 tcgcatgcct atggcgcggc gtatgacaat cccgacctga tcgtggcctg cgtcgtcgga
     1981 gacggtgaag ccgaaaccgg tccgctggcc acttcatggc actcgaataa gtttctgaat
     2041 ccactcgctg atggcgtcgt gctccccatt ctgcatctga acgggtacaa gatcgcgaat
     2101 ccgtgcgtgc tggcccgcat cagtcatcaa gagctggacc aactcttccg gggctacggc
     2161 tacacaccgt attttgtcga gggaagcgat cccgagaaga tgcaccaaac gatggcggca
     2221 acgctcgaca cgatcattga agacattcac cgcatcaaga cggaagcgcg acggacgggt
     2281 gccaagaagc gaccgctgtg gccgatgatc attctccgtt ctcccaaagg atggacctgc
     2341 ccgaaggaaa tcgatggcaa gcggaccgag gactactggc ggagccatca agtgccgatg
     2401 ggggagatgc acgagaaggc ggagcacgtc aaaattctcg agaaatggat gaagagctac
     2461 aagccggagg acctcttcga taaggccggc cgcctgaaat ccgaactggc ggagctgtca
     2521 cccaagggcg agcgccgcat gagcgccaat ccgcacgcga acggcgggtt gctgctcagg
     2581 gacctgcgtc tgcctgattt tcaagattat gcagccgagg tgaccaaacc cggcgcgacg
     2641 atggctgaat cgacgcgaca catgggaaag ttcttgcgcg acatcatgaa actgaatatg
     2701 gacagccgca acttccggct gttcagtcct gacgagaaca attcaaaccg ctggcaggat
     2761 gtgttggacg tgacgaatcg tgcgtggatg gccgaaagct atccatacga cgatcacctg
     2821 gcgccggacg gccgggtcat ggaaatgctc agcgagcacc agtgccaggg gtggctcgaa
     2881 gggtatctct tgacggggcg gcatgggttc ttctcctgct atgaggcgtt catccatatc
     2941 atcgactcca tgttcaatca gcacgcgaaa tggctgaagg tctgcaatga aatcccttgg
     3001 aggaggccga tcgcctcact caactatttg ctgagctctc atgtgtggcg gcaggatcac
     3061 aatggattca gccatcagga ccccgggttc atcgatcacg tcgtcaataa gaaggcggac
     3121 gtggtacgag tctatctccc gcccgatgcg aataccctgt tgtccgtgac ggatcattgc
     3181 ctgcggagcc gcaactacat caacgtcatc gtcgccggaa agcagccggc tccccaatgg
     3241 ttgacgatgg atcaggcgat caaacattgc tcggccggga tcggtatttg ggaatgggcg
     3301 agtaacgata aggaggggga tcccgacgtc gtcatggcct gttgcggaga cgtgcccaca
     3361 ctcgaaacgc tagcagcggt ggacatcatt cgacaacacg tgcctgcctt gaggatcagg
     3421 gtggtcaacg tcgtgaatct catgaaattg cagccgccga gcgaacatcc gcatggtctg
     3481 tcggacttcg atttcgacgc gctgtttacg aaagacaagc ccattgtctt cgcgttccac
     3541 ggctatccct ggttgattca ccgccttacc tatcgaagga ccaatcacaa gaacctgcat
     3601 gtccggggat ataaagagga aggcacgacg agcacgccct ttgacatggt cgtcatgaac
     3661 gatctcgacc gctttcatct tgtcgcggac gtggtggacc gcctgccgca actgggatcc
     3721 cgggccgcct atgccaagca ggcgatccag gaaaagctgc tggagcataa gcaatacatc
     3781 gccaggtacg gcgaagacat gccggagatc accgattggc aatggggcca ggcgagtggc
     3841 aagtcaaagc gcaaaagttc cacggaaggc gacaacgtct agtccaattt tgtcttcgtc
     3901 ggtggagggt tgttatgaca caggaaggac tcaaagactt gcttcaacaa tatggcgtgg
     3961 gacccatcca attttccggg acccacgacg cgctgtacga acggcatctc acattcgacc
     4021 acgtcgttga tgtgaaacgg gccgatgtgc gggaacagtt tgaagcggcg gcgcgctcca
     4081 ttcgcgatat catttcccag cgctggcttc ggacggaacg gacctatgcc gagaagaatc
     4141 ccaagcgggt gtattacttg tccatggaat tcctgctcgg cagatcgttc gccaataatg
     4201 tcacgaatgc cctgctccgt cctttggcga ccgaggccgc caagacgaag ggattggatc
     4261 cgttcgcgct gatcgaggcg gagccggatg ccggacttgg caacggcgga ttggggaggc
     4321 tcgctgcttg cttcatcgat tcgatggcga cgatggggat cccaggcatg ggctacggcc
     4381 tgcgctatga atacggaatc ttcaagcaga ccatcgtgga tggatggcaa tgcgaacagc
     4441 cggaccactg gctccgaaga ccggatccct gggaagtggc tcgtctggag gaagcggtcg
     4501 aggtgaagct gaattgctcg ttcgaactgc ggggaggcat gttacatccc gtcattggtc
     4561 ggccttcgac actgctgggc attcccttcg atcgaccggt cgtcggttac ggaggcaaaa
     4621 cgatcaatac gttgcgtctt tgggccgcga gcacgccgga ttatttcgac tttcagcaat
     4681 tcagccgcgg tgatttcgta ggcgccctgg ccgagacgct cacagcggaa acgctcaccc
     4741 gggtgctcta tcccgacgac tcgacgtcga tgggtcaggg gctgcgcttc cttcaggaat
     4801 atttcctggt ggcctgctcg ttgggcgatc tcgtgcggcg tttccgccgc tccaatacac
     4861 aatggcaggc tcttcctgac aaggtggcgg cgcaaatgaa tgatacccac ccggccatgg
     4921 ccgtggccga actcatgcgg attctcttgg acgaggcgca cctcgagtgg gaggaggcat
     4981 gggatctgac acaacggacg ctggcgtata ccaaccacac gctcctgccc gaggcgttgg
     5041 agaagtggcc gctcgaatgg tttgaagcga tgctgccgag acacctcgaa attattcttg
     5101 agatcaaccg ccgactcatt gagggcgtcc ggcatcgctt tcccggcgac gacggacgta
     5161 tcgagcgagt gagcctcgtg gaggaaggcg cgaggcggaa gatccgtatg gccaatctgg
     5221 cgatcgtcgg atctcacagc acgaacggcg tggccgccat ccattctcga ttgctgcgga
     5281 ctatgaccgt caaggacttg gccgaactct atcctgaacg gttcaacaac aagaccaacg
     5341 gcgtaacgcc gcgccgctgg ctcctgctgg cgaaccccgg gctcgcgaat gcgatcaccg
     5401 aagccatcgg cgaccggtgg attaccgatc tgagccagct caccaagctc aaggcactcg
     5461 ccggcgatcc ggcgtttcgc gattcgttcc ttcgcgcgaa gcgcgaagcc aaagcgcaat
     5521 ttgctcagtg gctcgaaacc acttccgggc agcacattaa tcccgacagc atgttcgatt
     5581 gtcaggtcaa acgtattcac gaatataagc ggcagttgct gaatgccttg cgcatcgtcg
     5641 tgctgtataa ccgcttgcga gaaaatcccg atcagcccat ggcgccgagg acattctttt
     5701 ttgccggcaa ggctgcgccg gcctaccact tggccaaggt catcatcaag ttcatcaaca
     5761 acctggccgg cacgatcgac ggagatcctc ttgttcgcgg taggatcaag gtggtcttcc
     5821 tgccggagta ttgcgtgtct cttgccgagc ggttgattcc ggccactgat gcctccaatc
     5881 aaatctccac ggccggatat gaagccagcg gaaccagcaa tatgaagttc atgatgaatg
     5941 gcgcgctgac gataggcacc cgtgacggag ccaccatcga aatggccgaa gaagcgggag
     6001 aggaaaattt ctttctgttt gggttgactg cggagcaggt cgccgccagc cgaaattggt
     6061 acaaccctcg gtggcattat gatcacgagc cggaaacgag agcggcgctc gacttgatct
     6121 tctcgaatta cttcagccgg aatgaaccag ggacctttgc ggctctggag caggcactgt
     6181 tgacgggggg cgatcactac atgcatctgg cggatctgtc atcctacgtg aatgcggatc
     6241 gggagctgtg tcacctttac agcgactcaa agcaatgggc ccataaggcg attctgaata
     6301 tcgcgggctc gggaaaattc tccagcgatc ggacaatcgc cgaatatgcc gcggggatct
     6361 g
//
LOCUS       DASSCQ010000120         4382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1574368, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000120 DASSCQ010000000
VERSION     DASSCQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574368"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VFH05_08225"
     CDS             <1..1207
                     /locus_tag="VFH05_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678240.1"
                     /translation="LTVEGDGIAFARLVASRYGAGVALFERFATARLTMSDHTKVDIA
                     STRRESYADPAALPEVRPARLEEDLFRRDFTMNAMAIELNDAQWGRLHDPYGGRKDLK
                     TKVIRVLHAKSFVDDPTRIFRAIRFAERFGFHLEATTHRLLKQAAETNIVAQLSGPRL
                     ANEIFMLMRERHPERALAALARRHLLRFLHPRLTYSRQARLLMTVLPRGIGWWERQCP
                     RNPIDRSLVWFMALLESASGAIITRIAERLQLSTLQARTLEWAGTNTSNASETISSSN
                     SLRPSQVYRLLKDMPNEAMVLAMSKTLLSHDRARVGRAGRRFVRFLARDRQVPTTVNG
                     DDLVQFGLRPGPQFKRILDRLLTERIDGTIKTAVEERVLARRLVAATLRASQVGANGH
                     RSRSSRTGR"
     gene            complement(1146..1739)
                     /locus_tag="VFH05_08230"
     CDS             complement(1146..1739)
                     /locus_tag="VFH05_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678241.1"
                     /translation="MLSARIRRTSRAAYLLPLILFFACSTFVYEPAPVVPTTLPLPYS
                     ATFRLAELEAYNVEPGATMVADPRIENKVRGVRQPLSKAKKEWERSIADYVAARKTFT
                     YLSADSQTDLDLSMRLNIYVDPSVNSEFARVYVARIDATLAEGSSKRPLSKYLGFGKA
                     AGEDDDRGPVNLAVQAALNDLFGKIENDVRLRRPGLL"
     gene            1832..2371
                     /locus_tag="VFH05_08235"
     CDS             1832..2371
                     /locus_tag="VFH05_08235"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET6678242.1"
                     /translation="MASPLTHALVGATLAVACHFPWKLWVVGVASALLPDLDAAGYWL
                     GVPYGSFWGHRGFTHSIVFAVLASGLFARWISCWTGMTVVRVWTYCVVAMLSHGILDA
                     MTDGGLGVAFFSPMDQNRYFLPIRPIHVASMSLRDLLGPHGLDVLTSECVWVWIPCAV
                     VASLLWWFGRKRRPVSSSE"
     gene            complement(2355..2981)
                     /locus_tag="VFH05_08240"
     CDS             complement(2355..2981)
                     /locus_tag="VFH05_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678243.1"
                     /translation="MDDHQPPRTLSYDEKHAAEAAFSGRPFNPRWSARARLVYDGILN
                     SLPASARLEEPSSTDFVTESASTEHAEDRPVDRGDALPASIPFQKAIDSGALIDVTPT
                     AKQVGLSFSVTVTKPLWDAGIAPVQTLTSEEQAARLRDVLMAFRLRLAAQPALSPVID
                     FPALLNFPPNTIPQPFPLLALVQPDEQNQAMITLLLPSEMATTIIPMN"
     gene            complement(3029..>4382)
                     /locus_tag="VFH05_08245"
     CDS             complement(3029..>4382)
                     /locus_tag="VFH05_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6678244.1"
                     /translation="ALRSPCPLRGFIRGHSANNDRCSHSQRQLVSRQSWWQGVRRDQW
                     LVLFVAWLGWVFDAMDATIYAIVLHPALHELLHTSSGRPTAEHIGWYGGIIFSIFLIG
                     WAIGGITFGVLADRFGRTKILIATILMYAVFTGAAALSQEWWHLALYRFLTALGIGGE
                     WAAGAALVAESWPEEKRAKAAGILQSAWAVGFFLAALCNLALKDAYGWRGLFLIGILP
                     AFVALLIRWWVKEPPRWRHAHAQGIVPLSAIFKGDLKRATLVGSALAFVAVFGLWGST
                     NWAPTLVRELPEFQGADSAALTRSVSYAIMALNTGAIFGYLAFGPLADRFGRRAVFAF
                     MCIGSFIMLPTTYLLPSHYSGVLLLLPVLGFFNNGIFSGFPIYLPELYPTRLRATGAG
                     FCFNAGRILASASPFMTGWLVTTLGTFGRAASTVALIYLVGLVVLWFAPETKGKPLTD
                     "
BASE COUNT          938 a         1235 c         1320 g          889 t
ORIGIN      
        1 cctgaccgtg gagggtgacg gcattgcatt tgctcgcctt gtggccagcc gctacggcgc
       61 cggtgtggct ctgttcgagc gcttcgcgac cgcacggttg acgatgtccg accacaccaa
      121 ggtggacatt gctagcaccc ggcgggagtc ctacgccgat ccggcggcct tgccagaagt
      181 ccgtccagct cgcctcgaag aagatctctt ccgccgcgac ttcacaatga atgccatggc
      241 gatcgagttg aacgacgcgc agtggggacg cttacacgat ccgtacggcg ggcgcaagga
      301 tctcaaaacc aaagtcattc gagtgctgca tgccaaaagc tttgtggatg atccaacaag
      361 gatttttcgg gccattcgtt tcgccgaacg attcggcttt catttggagg ccacgactca
      421 ccgtctctta aagcaggctg cagagaccaa catcgtcgct cagctttctg gtccccgcct
      481 ggccaacgag atcttcatgc tcatgaggga acgccatccc gaacgagcgc tcgccgcatt
      541 ggcccggcgg catctgttgc gattcctgca cccgcgtctg acgtacagca ggcaggcgcg
      601 gcttcttatg actgtgcttc cccgagggat tggctggtgg gaacgacaat gcccgagaaa
      661 tccgattgat cggtcgctgg tgtggttcat ggcactattg gagagtgcca gtggagcgat
      721 aattacgaga atcgctgaac ggttgcaatt atctacactt caggcacgaa cgctcgaatg
      781 ggcgggaacc aacacaagca acgcgagtga gacgatttcc agttcgaact cccttcggcc
      841 ctctcaggtg tatcgactgc tgaaggacat gccgaatgaa gcgatggtct tggcgatgtc
      901 caagaccttg ctcagtcatg accgagcgag ggtggggaga gccggcagac gattcgtgcg
      961 atttcttgca agggatcggc aggtgccgac cacagtgaac ggggatgatc tcgtacagtt
     1021 cggactccgg cctggccctc aattcaagag aattctggat cggctgctaa ccgaacgaat
     1081 cgacggcacg atcaaaacgg cggtcgaaga gagggtgctg gcacggcgcc tggttgccgc
     1141 gacgcttaga gcaagccagg tcggcgcaaa cggacatcgt tctcgatctt cccgaacagg
     1201 tcgttgagcg cggcttggac ggcaagattg accggaccgc ggtcgtcgtc ctctcctgcg
     1261 gctttcccga atcccagata tttcgagagg ggacgtttgc tggatccttc cgccaacgtc
     1321 gcgtcgatgc gggccacgta gacgcgagcg aattcggaat tgacacttgg gtcgacataa
     1381 atattcagcc gcatggacag atcgagatcg gtctggctat cggctgacaa gtaggtaaac
     1441 gtttttctcg ccgctacata atccgcgatc gaccgttccc attctttttt tgccttgctc
     1501 agtggttgac gaacgcctct gaccttgttt tcgatgcgag gatcagcgac catcgtggcg
     1561 cccggttcca cgttataggc ttcgagttcc gccagacgga aggtggcgga atagggaagg
     1621 ggcaacgtgg ttggcactac cggagccggc tcgtagacaa aagttgagca ggcgaagaaa
     1681 aggatgaggg gcagcaaata ggcggcgcga gacgtccgac ggatccgcgc tgacagcatg
     1741 ctcgctccct tgtgttttcg tggtccggcg atgcatcaca ctacaacccg caatgcgagg
     1801 ttgcaagggg tgggaatgga ataggatcgc catggcctct ccccttacgc acgcactggt
     1861 tggtgcgact cttgccgtcg cctgtcactt cccttggaaa ctgtgggtgg tgggcgtggc
     1921 atctgccctg ttgcccgatc tcgatgccgc agggtattgg cttggcgtgc cctacggatc
     1981 gttttggggt catcgaggat tcacccactc catcgtgttt gctgtcctgg ctagtgggct
     2041 gttcgcgcgt tggatcagtt gctggactgg catgaccgtc gtccgagtct ggacctactg
     2101 tgttgtcgcc atgctttccc atggcattct ggatgccatg acggatggtg ggctcggagt
     2161 ggcattcttc tcgccgatgg atcagaaccg atacttcttg ccgattcgtc cgatccatgt
     2221 cgcctcgatg tctctgcgcg atcttttagg ccctcatggg cttgatgtgc tgacgagtga
     2281 atgcgtctgg gtatggatcc cctgcgctgt cgtggcgtca ctgctgtggt ggttcggtcg
     2341 aaagagacgt ccggtcagtt catcggaatg atcgtcgtcg ccatttcact tggcagcaac
     2401 aacgtgatca tggcttgatt ctgctcgtcc ggttgtacga gagcgagcag tggaaacggc
     2461 tgcggtatcg tgttgggcgg aaaattcaac aatgccggga agtcgatcac cggtgacagg
     2521 gcaggttggg ccgccaggcg aagcctgaag gccatcaaca cgtcgcgcaa tcgggcggcc
     2581 tgctcctccg aggtgagagt ctggaccggt gcaataccgg cgtcccaaag cggcttggtg
     2641 acggttaccg agaaagacaa accaacctgt ttcgcggtcg gcgtgacatc aattaacgcc
     2701 cctgagtcga tcgccttctg gaaaggtatg gacgcaggca aagcatcgcc ccgatcaaca
     2761 ggccggtctt ctgcgtgctc ggtggacgcc gattctgtga cgaagtcagt cgatgacggc
     2821 tcttccaacc ttgcggaggc cggcagggaa ttgagaatcc cgtcatagac gagcctggct
     2881 cgggcagacc aacgtgggtt gaacggacga ccgctgaagg ccgcttcggc tgcgtgcttc
     2941 tcatcgtagg aaagcgtacg agggggctga tgatcatcca taatactcaa ataaatcggt
     3001 ccaggcggaa gtcaagggtt cggcacaatt agtctgtcag cggtttgcct ttggtttctg
     3061 gtgcaaacca caggacgacc aagccgacca gataaatcaa ggccaccgta ctggctgcgc
     3121 gaccgaaggt accaagcgtc gtgaccagcc agccggtcat gaagggggag gctgaggcaa
     3181 ggatccggcc cgcattgaag caaaagccgg ctccggtggc gcggagccgc gtcggataca
     3241 gctccggcag gtagatcggg aatccgctaa aaatcccgtt gttgaagaaa ccgagaaccg
     3301 gtaaaagcag caagacgccg ctgtaatgag acggcagaag atacgtcgtg ggcaacatga
     3361 taaagctgcc gatacacatg aacgcaaaga ccgctcggcg tccgaatcgg tcagctaatg
     3421 ggccgaaggc aagatatccg aagattgctc cggtattcag cgccataatg gcatagctga
     3481 ccgatcgtgt gagtgccgcg gagtccgctc cctgaaattc cggcagctcc cgcacgagcg
     3541 tcggggccca gttcgtcgat ccccaaagac cgaacaccgc gacgaaagcc agggcagaac
     3601 ccacgagcgt cgctcgtttc agatcccctt taaaaatggc cgacaatggg acgattccct
     3661 gtgcatgggc gtgcctccag cgtggtggct ccttcaccca ccacctgatc agtaaggcga
     3721 cgaaggccgg caagatgccg atgaggaaaa gaccccgcca tccgtacgcg tctttcaggg
     3781 caagattgca gagagccgcc agaaaaaatc ccacggccca ggcggactgt aaaattcctg
     3841 ccgccttggc ccgtttctct tccggccacg attccgcgac aagggccgct cccgcggccc
     3901 actctccgcc gattcccagt gccgtgagaa accggtagag cgccagatgc caccactcct
     3961 gtgagagggc ggccgcgccg gtgaacacag catacatgag gattgtcgct atgaggatct
     4021 tggtgcgtcc aaagcgatcc gccaggactc caaacgtgat cccgccgatt gcccagccga
     4081 tcaaaaaaat ggaaaagata atcccaccgt accagccgat gtgttcggcg gtcggtcggc
     4141 cgctcgaggt gtgtaacaat tcatgcaggg ccggatgcag aacgattgca tagatcgtgg
     4201 catccatggc atcgaagacc cacccgagcc atgcgacaaa caacacgagc cattgatctc
     4261 gacgcacacc ctgccaccag gactgcctgc tcaccaattg cctctgggag tgactgcacc
     4321 gatcgttgtt ggctgagtgt cctcggatga atccacgaag gggacatggg ctgcgcaagg
     4381 ct
//
LOCUS       DASSCQ010000121        13192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1020931, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000121 DASSCQ010000000
VERSION     DASSCQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13192
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020931"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFH05_08250"
     CDS             <1..209
                     /locus_tag="VFH05_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678245.1"
                     /translation="RLLTVEVDNKGWTRMNKAQKLEFLDRVNGAALSANGGIAIEIHV
                     SMSGAKVASSMFSAGQQSLRLLLE"
     gene            complement(431..1822)
                     /locus_tag="VFH05_08255"
     CDS             complement(431..1822)
                     /locus_tag="VFH05_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HET6678246.1"
                     /translation="MAFSSISRYWIIWKAAWRAQSTRQPGMASGGRTTEFLPAVLEIE
                     QTPPSPIGRAISWTIIGVFISGVLWATVGWIDIVATAQGKIIASGYSKVIQPYETGVI
                     ASIHVLDGQVVTRGQVLIELDPTQNRADRDRASNEYRAAKVDAARLRALIAGRSTFEE
                     PKDGDPPYVALQQQLLRDQLTEYEARVGAARDLIEQRRAALEETKENVRRLESTLPME
                     EEKADTFKKLLEQEAASKLDYLQAEQQRIDKTQELAGQRKKVMQDAAALSEAQHNYRV
                     TMTEFQQAKQAELSTIETKAASLMQDVTKAGQRTDLQRLMSPIDGVVQQLAVHTVGGV
                     VTPAQELLIVVPQDHPVEVEAQVENKDVGFVKEGQEVEIKIETFPFTLYGTIPGKVLS
                     VSDDAAPIEKVGLVYPTRVSMDRSTIEVDGKQVNLSPGMVATVEIKTGKRRLIEYLLS
                     PLLKSVKESLRER"
     gene            complement(1840..4023)
                     /locus_tag="VFH05_08260"
     CDS             complement(1840..4023)
                     /locus_tag="VFH05_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HET6678247.1"
                     /translation="MTTNAPPQSRPQSSARSATGSHTDTGLVSLLILARFYDLPADGP
                     QLRQLYAQPGQPLSDTELLRAAKHLGLKAGMIQTQWTKLSGTPFPAIAKLGNGRHVVL
                     AKINGEKVLIQDPVEGRPLVVSREQFESNWTGELFLFAKRANLRRQDLKFDLSWFIPA
                     IVKYRQFLGEVLVASFVLQLFALLTPLFTQVVIDKVLVHKGFTTLHVLAVGMVTLAMF
                     DAVLGGLRTYLFAHTTNRIDVGLGAQLFRHVLALPLAYFEARRVGDTVARVRELEHIR
                     QFLTSHSVTVVLDVVFTLVFLIVMWFYSHALTMVVMASLPLYAILSIAITPTIRARLH
                     ERFNRGAENQSFLVEAISGIQTVKALAVEPPLIRKWEEQLAGYVRASFRATSLITVAG
                     QIAGCIQKITTVAVLWVGAYRVIDGALSIGQLIAFSMLSAQVTGPLLRLVSLWQEFQQ
                     VGISVQRLGDLLNTQPEPSYKPNRTTLPQVAGQVSFEDVTFRYRPDSSEVLRRMSFSV
                     TAGKVIGIVGRSGSGKSTIAKLIQRLYVPERGRIVLDNVDLAQVDPAWLRRQVGVVLQ
                     ENFLFNSSVRDNIALADPGLAMEQVIQSAKLAGAHEFILELPDGYDTIVGEHGCSLSG
                     GQRQRIAIARALVANPRILIFDEATSALDYESEAVIRKNMVQICQGRTVFIIAHRLST
                     VRPADQIYVVEKGEIVEKGSHEELLRTNGFYARLCTLQDTAGARA"
     gene            4323..5858
                     /locus_tag="VFH05_08265"
     CDS             4323..5858
                     /locus_tag="VFH05_08265"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET6678248.1"
                     /translation="MELARLVDAVERVRATTKKTEKARTLAHLLRQTSGHETKLAALY
                     LTGSLPQGKIGIGWHLIQKAIQDVPVHGLPLTLSDVDKVVTVLAAERGEGSSERRVVE
                     LSRLTSRARPEERRFLSQLLLGELRQGALEGMLLEAIAIAAGLPPTEVRQASMFAPNI
                     GDLAQVALAEGSSGLARYSLRLFVPVAPMLANSAEDIEAALERHKPAAFEYKLDGARI
                     QVHKGGAEVRVFTRQLQDVTERLPELVQWAQALPVQEAVLDGEAIGLRQDGRPQPFQV
                     TMRRLGRVKDIAEIRETIPLTPFLFDALYVDGNSLLTQTYEYRTGVLESLAHGATVPR
                     LLTAQPEEARHFLAQALAAGHEGVMAKSPSAPYIAGQRGFHWIKLKEATTLDLVILAA
                     EWGNGRREGWLSNLHLGARDPESGGFVMLGKTFKGLTDEMLRLQTEKLLTLETHREGH
                     TVFVRPEIVAEIAFSDVQESPRYPAGLALRFARVKRYRPDKSPQEADTIQTVKALFQT
                     QRA"
     gene            5955..6227
                     /locus_tag="VFH05_08270"
     CDS             5955..6227
                     /locus_tag="VFH05_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678249.1"
                     /translation="MRKMFLMILVGMAIGGTAWSVLAGETKARMEDSKGEMKADFEEA
                     KGGAKALKEEAKGNDTKAEVERAKGKVKGGGERVKGKSKAAKERAK"
     gene            complement(6291..6611)
                     /gene="sugE"
                     /locus_tag="VFH05_08275"
     CDS             complement(6291..6611)
                     /gene="sugE"
                     /locus_tag="VFH05_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HET6678250.1"
                     /translation="MAWVILVLAGLFETGWAIGLKYTEGFTRFWPTVWTVFAMIVSLW
                     LLGIAMRTLPVGTAYSVWVGVGAVGTVALGIALLEEPVNAARLASVGLIIAGIIGLKL
                     ATPA"
     gene            6749..7120
                     /locus_tag="VFH05_08280"
     CDS             6749..7120
                     /locus_tag="VFH05_08280"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HET6678251.1"
                     /translation="MTHPRYRWILVACAPLILSLSACSVTETISNFLSSTSGRSWWTE
                     DGLIKTDQRVNAFMVFNFENIKQDMARGQGEYLDSLTTLMAVPPDRRAAFNAYAQSRY
                     PLVIQHRSSPEETLLLLVEHS"
     gene            complement(7163..11464)
                     /locus_tag="VFH05_08285"
     CDS             complement(7163..11464)
                     /locus_tag="VFH05_08285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6678252.1"
                     /translation="MPLTGFHPLIADWFQSSIGVPTDVQAQAWPAIRSGRDALIAAPT
                     GSGKTFAAFLSSIDQLFVQALARDLDDRTHVLYVSPLKALSNDIQKNLQQPLADIGQA
                     ALQAGLLMPELRVLVRTGDTPMSDRQQMLKRPPHILVTTPESLFILLTAEKSRRLLQT
                     VRTVIVDEIHALAPNKRGAHLALSLERLEALTLTKPQRIGLSATQRPIDLVARFLTGD
                     RPRPTVIDVGHKRQLDVAVEVPKDELSAVVSNAIWSDIYDRVATLVREHRSTLVFVNT
                     RRLAERVAHHLEERLRDLGPQSVAAHHGSLSRQIRLSAEERLKSGRTRVVIATASLEL
                     GIDVGTIDLVCQIGSPRAIATCLQRIGRAGHWIKAIPKGRLFATTRDELLECAALIRA
                     MRSGTLDRISVPPAPMDILAQHIVAAAATQAWTEAELYALIRRAYPYRQLQRSEFDVV
                     VRMLADGIATNRARGHAYLYHDRINHVVKGRRGARLAAITSGGAIPDTANYAVVAEPE
                     GTVVGSVDEDFAVESLAGDIILLGNTSWRIKGVEMGRVRVEDAHGAPPSIPFWRGEAP
                     SRTAELSAEVADIRRDIAERALDAEHSAVHWLKQECALDQRGAQQAIEYVVGSKAILG
                     VVPTQTTIVAERFFDESGGMQLVIHAPFGGRITKAWGLALRKRFCVTFDFELQAAATD
                     NGLVISLGEKHSFPLESVFGYLRSESVREVLLQAVLQAPMFATRWRWNASRSLALLRF
                     VNGRKVPPQIQRMRAEDLLAAVFPDAIACQDNMTGERERRIPDHPLVQETLRDCLTEA
                     MDVDGLTDVLGGIEAGTIRCVAIDTPSPSPFSHEILNANPYAFLDDAPLEERRARAVA
                     LRRTLPGEMAEGVGSLDSAAIDEVVRESWPVVRNADDLHDALLTLMWVPAPEVRGWQQ
                     FLPELVQSGRATEWAIDNGKPMTVVQRDHKTIGWVATEQLANVQRALAGDDEATVDAI
                     VHGWMESIGPVTTEELARRLWLQTDRVNASMIRLESQGHVLRGRFRPRSGDDGGLPEW
                     CHRRLLARIHRLTIGTLRKQVEPVATADFMRFLFQWQHVTSGSRQHGEPGLLEVIRQL
                     SGFEAAASSWDSQLLRTRMAKYEPHLLDRLCLSGAVSWGRLSPHPRLMDTSDRERLRR
                     ITPTSVAPISLFPREDSTWLLEVFQTGLWHGPDPFAALSAVAQNVREVLQQRGASFFA
                     DLVQATGHLPAEVEGGLWELVAAGLVTADGFDNLRALMNPRRRRAEGLERLRRPRHAA
                     GRWSLLRTGSSVSARTGEELHSVSASTEKVARQLLRRYGVVFRDLLAREVLVQSWRDL
                     LVVYRRLELAGDVRGGRFVSGFVGEQFALPEAVEALRAMRKTTGGPTAAEIKLSACDP
                     LNLAGVILPGARVPAVPSTFIIFRDGVPVRTLSGNRQRGDDEEVLALARGAAAGS"
     gene            complement(11535..12953)
                     /locus_tag="VFH05_08290"
     CDS             complement(11535..12953)
                     /locus_tag="VFH05_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678253.1"
                     /translation="MSTRSILSLLVAASLCLTGCPSDNGGGGAVPASGGTVVYLADQD
                     IPGVFELYLEGSGTKLNPALAVGRTITSFALTPDAAAVVYIADQDQDDVFEIYRVNLA
                     SPGASTKLNGALVAGGDVVQLAVTADGTAVVYRADQTQDEVFELFRTLFNGGTNSKLN
                     PPYTISQDVETFVLLPDSSGVIYRADQATDGVSELYRVLFATAGTSNPLINPPLFGGQ
                     NVDTFAATPDSANVVYIANRPIGSNQIFIVPAGGGGSIPLNGPLVANGNVTAFSVTPD
                     GLSVVYRADQDIDEVYELYRTVILSAPTNVKLNPSLAAGGDVVSFAVTPDSTSVVYTA
                     DQTTNDIVEIFRTVFSGNVNSQLNPSFPATADVVDFALFPNGSGLVYKADQTTDGVDE
                     LYRVVFGFPGAPRVNPALVIGQNVATYAVAPDSNSVIYRANQDNAGIVELYRAAFSSA
                     GTSSKLNAPLVVGENVASFAVR"
BASE COUNT         2830 a         3990 c         3723 g         2649 t
ORIGIN      
        1 accgactgct gaccgtcgaa gtcgataaca agggctggac tcggatgaac aaagctcaaa
       61 agctcgagtt tctcgacaga gttaatggcg cagccctgag cgcgaacggt ggcatcgcca
      121 tcgagattca cgtgtcaatg agcggagcga aagtagccag ttccatgttt tctgcaggac
      181 aacaatccct tcggctgttg cttgaatagt gaccgggaca tcggctcccc accatgggga
      241 ggggagccgc catattgtgt tcgcaaatcg atttccaatc agacctataa tcaagatgat
      301 ccacagcgac ggcacccgct cccctgcgat cgactttcca tctcacatca gtccagtcac
      361 aatgccgatc agacactgaa caggctcgca agggtaaaag agggatgaca ccggcgggct
      421 ctgcgtattg ctaccgctcc cgcaggcttt ctttaacaga cttgagcaga gggctaagaa
      481 ggtattcgat caggcggcgt ttccccgtct tgatttccac cgtagcgacc atacctggcg
      541 acagattcac ctgcttgcca tcgacttcga tggtggagcg gtccatgctc actctggtcg
      601 gatagaccaa tcccaccttt tcgatcggcg cggcatcgtc tgaaaccgac aacactttgc
      661 cgggaatcgt accgtagagg gtgaacggaa atgtttcaat ttttatttcg acttcttggc
      721 cttctttcac gaagccgaca tctttgtttt ccacctgagc ctccacctca acgggatgat
      781 cttgcggcac aacgattaat aattcctgag caggcgtcac cacaccaccg accgtgtgca
      841 ccgccagctg ctgcacgact ccgtcgatcg gactcatcaa ccgctgcaaa tcggtcctct
      901 ggcctgcttt ggtcacgtcc tgcatcaaag acgcagcctt cgtctcgata gtcgagagct
      961 ccgcttgttt ggcctgctga aactctgtca ttgtgacgcg atagttgtgt tgcgcctccg
     1021 aaagtgcagc cgcgtcctgc atcaccttct tgcgctgacc ggccaactcc tgcgtcttgt
     1081 cgattcgctg ctgttcggcc tgaaggtaat ccaacttgct cgcagcttcc tgttcgagca
     1141 gcttcttgaa tgtatcggct ttctcttctt ccatcggcag ggtggactcc agccgtcgaa
     1201 cattctcctt cgtctcttcc agtgcggccc tgcgttgttc tatcaagtct cgcgcggcac
     1261 cgactcgggc ctcatattcg gtcagctggt cacgcagcaa ctgctgttga agtgcgacgt
     1321 acggtggatc tccatctttg ggttcttcga atgtgcttct gccggcaatc aatgcccgca
     1381 agcgagccgc atcaaccttt gctgcccgat attcattcga agcccggtcg cgatcggcac
     1441 ggttttgggt tggatccaac tcgatgagta cttgcccacg tgtcacaacc tggccatcca
     1501 ggacatggat cgatgcaatg acaccggttt cgtagggctg aatgactttg gaatacccgc
     1561 tggcaatgat ctttccctga gccgtcgcga ctatatctat ccatcctacg gtggcccaca
     1621 acactccaga gataaacacg ccgatgatcg tccaactgat ggcgcgacca atgggcgagg
     1681 gtggtgtctg ctcgatctcg agcaccgccg gcaggaattc cgtggtccgc ccgccgcttg
     1741 ccatcccggg ttgccgggtc gattgggccc gccaggccgc cttccaaatg atccagtatc
     1801 gtgagatcga gctgaatgcc ataactctac attttcggtc taagccctgg cgcctgcggt
     1861 gtcttgcagc gtgcacaagc gcgcataaaa cccgttcgtt cgcaggagtt cttcgtgcga
     1921 ccctttttcg acgatttcgc ccttttccac gacatagatt tgatcggctg gccggactgt
     1981 gctgagccgg tgtgcgataa tgaagaccgt gcgtccttga caaatctgca ccatgttctt
     2041 tcggatgacg gcttcggatt cgtaatccaa cgcgctggtt gcctcatcga aaatcaaaat
     2101 acgagggttc gcaaccaatg ctctggcaat ggcaatccgt tgccgctgcc cgcccgagag
     2161 cgaacagccg tgctcgccga cgatggtgtc atagccatcc ggcaattcga gaataaattc
     2221 gtgcgcgccc gccaatttcg ccgattggat gacttgctcc atggccagcc ctggatccgc
     2281 caaggcgatg ttatctcgca cggaactgtt aaagagaaaa ttttcttgaa ggaccacccc
     2341 cacctgccga cgtaaccagg ccggatccac ttgcgcaaga tcaacattat ccaagacgat
     2401 gcggcctcgc tctggcacat acagccgttg gatgagcttg gcgatcgtgc tcttgccgga
     2461 cccagagcga cccacaatgc cgatcacctt tccagcggtg accgagaacg acattcttct
     2521 cagcacctcc gagctgtcag gccgatagcg aaacgtcaca tcttcgaaac tcacctgtcc
     2581 cgccacctgg ggcaatgttg tgcgattcgg cttatacgag ggctccggtt gcgtattgag
     2641 caggtcaccc aatcgttgca ccgaaatccc gacttgttgg aactcctgcc agagactgac
     2701 cagacgcagc agcggtcctg tcacctgcgc cgacagcata ctgaacgcaa tcaactgccc
     2761 gatgctgaga gccccgtcga tcacccgata ggccccgacc cacagcaccg cgacagtcgt
     2821 gatcttctga atgcagcccg caatttgacc agccacggtg atgagactcg ttgcgcggaa
     2881 actggcccgc acatagcctg ccagctgctc ttcccacttg cgtatcagcg ggggctcgac
     2941 cgccaacgcc ttgacggtct gaatcccgct gatggcctca accaaaaaag actggttctc
     3001 cgcgccccgg ttgaaccttt catgcagtct ggcgcgaatc gtcggcgtga tggcgattga
     3061 caggatggca tacaagggca gggatgccat cacgaccatc gtcagcgcgt ggctgtaaaa
     3121 ccacatgacg atcaggaaca ccagggtgaa caccacgtcc aggacgaccg tcaccgaatg
     3181 gctcgtcaga aattgccgga tgtgctccag ttcgcgtacg cgcgccacag tgtcgccgac
     3241 acgccgtgct tcgaagtagg ccagtggaag ggcgaggaca tgacggaata attgagcacc
     3301 caacccgaca tcgatccggt tggtcgtatg cgcaaataga taggtgcgga gaccgcccag
     3361 cacggcatcg aacatggcca gcgtcaccat gccgaccgcc agcacatgca gggtcgtgaa
     3421 tcccttgtgc acgagcacct tgtcgatcac gacttgcgta aagagaggag tcagtaaggc
     3481 aaatagctgc agcacgaacg aggcgacgag gacttcacca agaaattgcc gatatttgac
     3541 gatggcagga atgaaccagg acaagtcgaa tttcagatcc tgtcgacgaa ggttcgcccg
     3601 cttcgcaaac aggaagagtt cgcctgtcca attcgactcg aactgttcac gggagactac
     3661 gagcggacgc ccttcgacgg ggtcttgtat cagcaccttc tcgccgttga tcttggcgag
     3721 cacgacatga cgcccattcc cgagctttgc aatcgcggga aacggcgtgc cggacaactt
     3781 cgtccactga gtttgtatca ttccggcctt gagtcctaag tgcttggcgg cacgaagcag
     3841 ttccgtgtcg gagagcggtt gtccgggctg cgcatacaat tgccgcaact ggggtccatc
     3901 ggcaggtaag tcatagaacc gggcgaggat cagaaggctg accagcccgg tgtctgtatg
     3961 cgaaccggtt gctgaccggg ctgatgattg aggacggctt tgaggcggcg cattcgtcgt
     4021 catcgtgaca agatcaccga agtccaggaa acggattcaa gagagcgaca acgacgaata
     4081 ccattatttt acgctttcct aactttcgga gggatgcgac tgtatcgaac gcttcggctt
     4141 cctgtcaact gatttattca gcgttttatc ctagggactt atctgatcat tacgctctac
     4201 cgcccttact tagatcaacg gctcgcattc tctacgaatc cgcactgacc atggtttgtt
     4261 ccaatactcg cgctccgtag cgtccatttc tttcatcacg cggccagcct atataatgga
     4321 gcatggaact ggcccgcctt gtcgatgccg tcgaacgcgt tcgtgccacc acgaaaaaaa
     4381 ccgaaaaggc tcgtacgctg gcgcacctcc ttcggcagac aagcggccat gaaaccaagc
     4441 ttgcggcgct gtatctcact gggtctttgc ctcaaggcaa gatcgggata ggctggcacc
     4501 tcattcaaaa ggccatacag gatgtccccg tccatggtct gcccctgacc ttgagcgacg
     4561 tggacaaggt ggtgaccgtc ctggcagcag agcgtggcga ggggtcttcc gaacgacgcg
     4621 tcgttgaact cagccggttg accagccgcg cgcgaccaga ggaacgccgg tttctgagcc
     4681 agctgctcct cggtgaactc aggcagggcg cgctcgaagg gatgctgctg gaagcgatcg
     4741 ccatagccgc tggattgcct cctaccgagg tgcgacaggc ctccatgttc gccccgaaca
     4801 tcggcgatct cgcgcaggtg gccctcgcag aaggatcgag cggactcgcg cggtattcgc
     4861 ttcgactgtt tgttcccgtg gcgccgatgc tggcgaacag cgcagaggac atcgaggccg
     4921 cgttggagcg tcacaagcct gccgcatttg aatacaagct ggatggcgca cgcattcagg
     4981 tgcacaaggg cggagccgag gtgcgcgttt ttacacggca gctccaagat gtgaccgagc
     5041 gactgccgga gctcgtccag tgggcacaag ctctcccggt ccaggaggca gtcctagacg
     5101 gcgaggcaat cgggctgcgc caggacggca gaccacagcc attccaagtg accatgcgtc
     5161 ggctcggccg cgtgaaagac atcgctgaga tacgcgagac gattcctttg acaccgttct
     5221 tgttcgatgc gctgtacgtt gacggcaatt cacttctgac acaaacgtac gaatatcgca
     5281 ccggggtatt ggaatcgtta gcccatgggg caactgtgcc acgtctgctt accgcacaac
     5341 cggaagaagc ccgacacttt ttggctcaag ccctcgccgc cggtcacgag ggcgtgatgg
     5401 cgaagtcacc gtccgctcca tacattgccg gtcagcgagg gtttcattgg ataaagctca
     5461 aggaagccac caccttggat ttggtcatcc ttgccgccga atggggcaat ggtcgtcgag
     5521 aggggtggtt gtcgaatctg catctcggtg cccgtgatcc cgaatctggc ggattcgtca
     5581 tgcttggcaa aaccttcaaa ggcctgaccg atgagatgtt acgcctgcag actgagaaac
     5641 tgctcactct cgaaacccac cgggaaggac atacggtatt cgtccgccct gaaattgtcg
     5701 cagagatcgc ctttagcgac gttcaggagt cgccacgtta tcctgccggt ctggctcttc
     5761 gctttgccag agtcaaacgc taccgtccag acaagtcacc gcaagaggca gatacgattc
     5821 agacggtcaa ggcacttttt caaacccaac gagcttgacc atacttccca gcctttttcc
     5881 tagggaagac tctagtatga gctgtgtagt aatgccgtta gaaccaagat agaagtcttc
     5941 tcttaggagg tattatgcgc aagatgttcc tgatgatcct ggttggcatg gctataggtg
     6001 ggacagcctg gtcggttctg gcgggcgaga ccaaggcgcg gatggaggat agcaagggcg
     6061 agatgaaggc ggattttgaa gaagccaagg gaggggccaa ggctctgaag gaagaagcaa
     6121 aaggcaatga cacgaaggct gaggtggaac gggctaaagg caaggtaaaa ggcggcggcg
     6181 agcgggtgaa gggcaagtcg aaagcagcga aggaacgagc caagtagccg tcgtagtacg
     6241 acttcgacgc cgccagcacc ggccgacgtt tgaaatgcac ctgaacaaga tcaggcaggt
     6301 gtcgcgagct tcaggccaat aatgccggca atgatgagac cgacgctggc caggcgggcc
     6361 gcgttgaccg gctcctcgag gagggcaatc cccaacgcga cggtccctac agccccgact
     6421 ccgacccaca cgctgtaggc cgtccccaca gggagcgtcc tcatagcaat tcctaacagc
     6481 catagactca cgatcatggc gaacacagtc cacaccgtgg gccagaagcg agtgaaaccc
     6541 tccgtgtatt tcagtcctat cgcccacccg gtttcaaaca acccggcgag aaccaaaatc
     6601 acccatgcca tagaattctt ctcctcgtta atcactcaac tttttcagcg gctgttggcg
     6661 gcgcatggct tgatcctcca tacgttctgc tctagagtga agaggagaca gatttccaac
     6721 gatcacttac catcgtagga gggttgccat gacacaccca agatacaggt ggatcctcgt
     6781 cgcttgtgcc ccactcatat tgtctctctc ggcctgcagc gtgaccgaga cgatcagcaa
     6841 tttcttgtcg tcgacttccg gccggtcctg gtggacagag gacggactca ttaagacgga
     6901 tcagagggtc aatgctttca tggtattcaa cttcgaaaac atcaaacagg acatggcaag
     6961 agggcaaggc gaatacctcg attcgcttac cacattgatg gcggtcccgc ctgatcgtcg
     7021 ggctgcgttc aatgcctatg ctcaatcgcg ctatcctctt gtcatacaac accgcagcag
     7081 ccccgaagag acactactgc tgctcgttga acattcctga gcgggaagcg gagacgagag
     7141 gccggccggg attcgtcagt gcttacgacc cggctgcagc gccgcgagcg agtgccagca
     7201 cctcttcatc gtctccgcgc tgccgattcc cgctgagggt tcgaacgggc acaccatcgc
     7261 ggaatatgat aaacgtgctc ggcaccgccg gcacgcgggc tcccgggaga atcactcccg
     7321 ccagattcaa tgggtcgcat gcggacagtt tgatttcggc ggctgtgggt cccccggtcg
     7381 tttttctcat cgccctcaag gcttcaacgg cctcagggag cgcaaactgc tctccgacga
     7441 atccagacac gaatcggccg ccgcgcacgt cgcccgctag ctccaagcgc cggtatacga
     7501 ccaacagatc tctccaggac tggaccagca cctcgcgagc taacaggtcg cgaaagacga
     7561 cgccgtagcg gcgaagcagc tggcgagcca ctttctcggt ggacgctgac acagagtgca
     7621 attcctcccc agtgcgagcc gagacggaac tccccgttcg gagtaatgac caccgtccag
     7681 cagcgtgtcg gggacgacgc agacgttcca gtccttcagc tcgacgccga cgcggattca
     7741 tcagggctcg caggttatcg aacccgtccg cagtcaccaa tccagcggcc accaattccc
     7801 ataatcctcc ctctacttcg gccggcaagt gaccggttgc ctgcaccaaa tcggcaaaaa
     7861 agctcgcccc tcgttgctgc aatacctcgc gcacattctg ggcaacggca ctcaaggcag
     7921 caaagggatc ggggccatgc cagagaccgg tctgaaacac ttccaacagc catgtgctgt
     7981 cctcccgggg gaagaggctg atcggagcca cactggtcgg cgtgatgcga cggagacgct
     8041 cccgatcaga ggtgtccatc aaacgcggat gaggcgataa ccgcccccaa ctgaccgctc
     8101 cgctcaagca caggcgatcc agcaaatgcg gctcatactt tgccatccgc gttcgcagca
     8161 actgtgaatc ccaactggac gcggcagctt caaatccact cagctgtcta atcacttcca
     8221 gtaaaccggg ctccccgtgt tgccgtgagc cggacgtcac atgctgccat tgaaagagaa
     8281 accgcatgaa gtcagccgtc gccacgggct caacctgctt gcgtaacgtg ccgatggtca
     8341 ggcgatgaat gcgggccaga agccgtcgat gacaccactc gggcaagccg ccatcgtcgc
     8401 cgcttcgggg ccggaagcgc cctcgcagca catggccttg agattcgagc cggatcatcg
     8461 atgcattgac tcgatccgtt tgaagccata accgcctggc caattcctcc gtcgtgacgg
     8521 gcccgatgct ttccatccaa ccgtgaacaa tggcatcgac tgtagcctcg tcgtcaccgg
     8581 ccagcgctcg ctgaacattg gccagctgtt cagtcgcgac ccatccgatg gtcttgtggt
     8641 cccgctgaac gaccgtcatg ggttttccgt tatcgatcgc ccactccgtc gcccgtcccg
     8701 actgcacgag ctccggcagg aattgttgcc agccgcgtac ctccggcgcg ggaacccaca
     8761 tcagcgtcag cagcgcatcg tgtaagtcgt cggcattgcg cacgacaggc caggattctc
     8821 gcacgacctc gtcgatcgcg gccgaatcaa gggaaccgac gccctcggcc atttcaccgg
     8881 gcaaggtacg gcgcagtgcc accgcacgcg cacgtcgttc ctcaagcggc gcgtcgtcga
     8941 gaaacgcata gggatttgcg ttcaagatct cgtgcgagaa cggagacggc gacggtgtat
     9001 cgatggcgac acaacgaatg gtgcccgcct cgattccgcc caggacgtct gtcagtccat
     9061 cgacgtccat cgcttcagtc agacaatcac gcaatgtctc ttgcaccagc ggatggtcgg
     9121 ggatgcggcg ttcccgctct cccgtcatgt tgtcttgaca ggcgatcgca tcgggaaaaa
     9181 ccgccgcgag cagatcctcg gctctcatcc gttgaatctg cggcggtact ttcctgccgt
     9241 tgacaaatct taggagcgcc aacgaccgcg aggcattcca gcgccaacgc gtcgcgaaca
     9301 tcggcgcctg gaggactgcc tggagcaaca cctctctcac gctttctgaa cggagatagc
     9361 caaacacgga ctccaatggg aaactgtgtt tttcaccgag cgaaatgacg agtccgttat
     9421 cggtggccgc cgcctgcagc tcgaaatcga acgtgacgca gaaccgcttg cgcaaggcca
     9481 acccccaggc tttggtaatg cgtccgccga acggcgcatg aatgacgagt tgcatgccgc
     9541 cgctctcgtc aaaaaaccgc tcggccacaa tggtggtctg cgtcgggaca accccaagga
     9601 tcgctttact gccgaccaca tactcgatag cctgttgcgc gcctcgttgg tccagagcgc
     9661 attcctgctt cagccaatgc actgccgagt gttcagcatc gagtgcccgt tcagcgatgt
     9721 cccggcggat atcggccacc tctgcggaga gttcagcggt tctcgacggc gcctcccccc
     9781 gccaaaatgg gatactcggc ggcgcgccgt gggcatcctc gactcggacc ctgcccattt
     9841 cgacgccttt gatccgccac gacgtgttgc cgagcaggat gatatcgcca gcgaggcttt
     9901 ccacggcaaa atcctcgtcc acggagccca ccaccgtccc ttcgggctcg gccacgaccg
     9961 cgtagttggc cgtgtccggg atggccccgc ctgaggtaat cgcagctaat cgcgctccac
    10021 ggcggccttt cacgacatgg ttgattcgat cgtggtagag ataggcatgt ccacgtgctc
    10081 ggttggtggc gatcccatcc gccagcatgc gtaccacgac atcaaattcg cttcgttgta
    10141 actgccgata gggatacgca cgccggatga gcgcgtagag ctccgcttcg gtccaggctt
    10201 gggtggccgc tgcggccacg atgtgctgcg caagtatatc catcggggcc ggcggcacgg
    10261 agatgcgatc taacgtgccc gacctcatcg cccgaatcag cgcagcgcat tcaagcaatt
    10321 catcccgcgt cgtggcaaag agccgtccct tgggaatcgc tttgatccag tgccccgctc
    10381 ggccaatccg ctgcaagcaa gtggcgatcg cacgaggcga tccaatctgg cacaccaagt
    10441 cgatggtgcc gacatcgatg ccgagttcca gcgaggcggt cgcgatcacc acgcgagtcc
    10501 tcccgctctt caagcgttcc tctgccgaca ggcgaatctg ccgcgaaagg ctcccatggt
    10561 gcgccgcgac actctgtggt cccaaatccc gaagccgctc ctcgaggtga tgggcgacgc
    10621 gttcagccaa ccggcgcgta ttgacgaaca ccaacgtgct gcgatgttcc cgcacgagcg
    10681 tggccacccg atcataaata tccgaccaga tcgcattcga gacgacggcg ctcaattcgt
    10741 ctttgggcac ctcgaccgcg acatccagtt gccgcttatg gccgacgtcg atgacagtgg
    10801 gacggggacg gtctccggtg agaaagcgcg cgaccaagtc gatagggcgc tgtgtggcgg
    10861 acaacccgat tcgctggggt tttgtcaggg tcaaggcttc cagtcgttcc aacgacagtg
    10921 cgaggtgggc gccgcgcttg ttgggagcca acgcgtgaat ttcatccacg atgactgtgc
    10981 gaacggtctg caggaggcgg cgacttttct cggccgtcag cagaatgaag agggattcgg
    11041 gagtggtgac cagaatatgc ggcggccgtt tgagcatctg ctgacgatcc gacatcgggg
    11101 tatcgcccgt ccggacgagc acgcgcagct caggcatgag gaggccggct tgcagtgcag
    11161 cttgtccgat gtcggcgagc ggctgctgga ggtttttctg aatgtcgttg ctcagcgcct
    11221 tgagcggcga gacgtacaac acgtgcgtgc ggtcatcaag gtcgcgggcc agtgcttgca
    11281 cgaacaactg gtcgatgctg gacaggaagg cggcgaaggt ctttcccgag ccggttggcg
    11341 cggcgatcag ggcgtcccgc ccagaccgga tcgcgggcca ggcctgagct tgaacgtccg
    11401 tgggaacgcc gatggaggat tgaaaccagt cggcaatgag gggatggaag cctgtcagcg
    11461 gcattccatt gatgctatgg cggcgtcagc gacgagtcaa ggccccggca cacgatcacg
    11521 tacgaacggc caccttagcg gaccgcaaag ctcgccacat tctccccaac gaccagcggc
    11581 gcgttgagct tcgaactcgt gccggccgag gaaaacgccg cgcgatagag ttcgacgatt
    11641 ccggcattgt cctgattcgc acggtaaatg acgctgttgc tgtccggagc caccgcatag
    11701 gtggcgacgt tttgcccgat caccagcgcg gggttgaccc ggggggcgcc ggggaagccg
    11761 aaaaccaccc gatacaattc atcgacaccg tcggtcgtct gatccgcctt gtagacaagg
    11821 ccgctgccat tcgggaacag ggcgaagtct acgacgtctg cggtggccgg gaagctcgga
    11881 ttcagctgag agttgacgtt tcccgagaac accgtccgga aaatctcaac aatatcgttg
    11941 gtcgtctgat ccgccgtata cacgacactg gtgctgtccg gtgttaccgc aaacgacacg
    12001 acatccccgc cggcggccag acttggattg agcttgacgt tggtaggtgc ggagaggatc
    12061 accgtccggt acagctcgta gacctcgtcg atgtcctgat cggcccgata caccacggaa
    12121 agtccatctg gcgtgaccga aaaggcggtc acgtttccgt tcgcgacgag gggcccattg
    12181 agtgggatgc tgccgccacc tccggctggg acaatgaaga tttggttgga gccaatcggc
    12241 cgattagcga tgtacacgac gttcgcgcta tcgggagtgg cagcgaaagt atcaacgttc
    12301 tgtcccccaa agagcggcgg atttatcaac ggattactcg taccagcggt cgcaaacagc
    12361 acacgataca attcactgac gccatcggta gcttggtcgg cgcgataaat cacgccgctg
    12421 ctgtccggca ggaggacgaa agtctccacg tcctgcgaaa tcgtataggg aggattgagc
    12481 ttgctgttcg tcccgccgtt aaacagtgtt cggaaaagtt caaacacctc gtcttgagtt
    12541 tgatcggcac ggtagaccac tgcagtgccg tcagcagtca ctgcgagctg gaccacgtcc
    12601 ccgcctgcca cgagcgctcc attgagcttc gtgctggcac cggggctcgc gagattgacg
    12661 cggtaaatct cgaacacgtc atcctggtcc tggtcggcaa tgtacacaac ggcggcagca
    12721 tcaggcgtca aggcgaaact cgtaatggtg cgtccgaccg caagcgcagg attcagtttc
    12781 gtgcccgagc cttcaagata gagttcgaag acgccgggga tatcttggtc ggcaaggtac
    12841 acgaccgttc cgcccgatgc cggaactgcc cccccgcccc cgttatcgct agggcaacca
    12901 gtcaggcata gcgaggcagc gacaagcaat gaaaggatgg atctggtgct catggcttca
    12961 ccaagggaac aaacccggga catcacaata cgtatgcccc ttagacgatt tataccatcc
    13021 ctcctgcagt gtgccaacga aacaccataa ttacgcccga gcaaactccg tcctggaggt
    13081 tggctcccag caagtcgtca atccgtcgtc ggaatgcata attaggggcg cgccgctgtc
    13141 gcgatggcgc gtatgatttc aatggattct tttcaccggc ccctggcaca ct
//
LOCUS       DASSCQ010000122        34489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1212287, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000122 DASSCQ010000000
VERSION     DASSCQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 34489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34489
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212287"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFH05_08295"
     CDS             <1..837
                     /locus_tag="VFH05_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6678254.1"
                     /translation="QLKNARLDFERYKGLYEQGFVPKQQLDTQEAIVRQSEGIVKADQ
                     GQIDNAKLQLAYSRITAPISGRVGLRQVDPGNMVRANDTNGLLVITQLTPIAVIFTIP
                     EDSLPAVLERLNAGQQLTVDAFDREHKKQLATGTLLTVDNQIDPNTGTVRLKAVFPNE
                     DGALFPNQFVNARLLLDVKRGTTLVPSAAVQRGPQGTFVFVVKGDHTVGVRAITVGVT
                     HGEESSVESGLVPDDLVVVDGAEKLRDGTKVEIRAPRDAPPPATGLPPTVGDEPRKGS
                     RS"
     gene            834..3950
                     /locus_tag="VFH05_08300"
     CDS             834..3950
                     /locus_tag="VFH05_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HET6678255.1"
                     /translation="MNPSRLFILRPVATTLSMAAIFLAGFIAYRLLPVSALPQVDYPT
                     IQVATFYPGASPDVMASSVTAPLERYFGQMPGLTQMTSTSSSGSSVVTLQFSLDLDLD
                     VAEQGVQAGINAASTFLPRDLPSPPVYSKVNPADAPILTLALTSETLPLSEVEDFAET
                     RFAQKISQMSGVGRVTISGGQRPAVRIQANPTTLAAYGLTLEDLRLALAAANVNQAKG
                     AFDGPRRASIINATDQLMVSADYRPLIVSYRNGAPVHLSDVADVIDDVENVKQAAWMN
                     DVPAVIVNIQRQPGANVIEVADRIKTLLPQLQSALPSSVHVTVLTDRTTSIRASVRDV
                     QFELMLAVALVILVIFLFLRTLSATVIPAAAVPLSLVGTFGLMYLMGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENIARYIEKGDTPFQAALKGSQQIAFTVLSLTISLIAVLIPLL
                     FMGDVVGRLFREFAITLSVTILISAVVSLTLTPMMCARLLRHRPQTEQGAFYRQTQRL
                     LDRVIAGYGRSLRWVLNHQQATLAVAVGTLMLTVLLYVVIPKGFFPVQDTGVILGITE
                     APQTISFGAMRDRQRAVARAVLEDPAVESLSSFIGVDGINTTLNSGRIQINLKPFKER
                     KLSASDIVQRLQSQVAGVGGITLFLQPVQDLTVENRVSRTQYQYTLEDADPEELNRWA
                     PALVESLQALPELRDVSSDQQNHGLAAMVNIDRNTAFRLGITPQLITDALYDAFGQRQ
                     VSTMFTQLNQYRVILEVMPEFQKGPQAMQFIGVRSFGGGQVPLNVFTTVTETTAPLTV
                     NRQAQFPSVTLSFNLAPRTSLGEAVKAIERVTQKVGMPPSIRGSFQGTAQAFRASLAN
                     EPLLILAALMTVYIVLGVLYESYIHPLTILSTLPSAGVGALLALMLSGTEFSVIALIG
                     IILLIGIVKKNAIMMIDFALDAERKDGKHPTEAIYEACLLRFRPIMMTTMAALLGALP
                     LAIGSGVGSELRHPLGIAIVGGLIVSQVLTLYTTPVVYLAFDRLARRVRAGTSQTPIS
                     EAVGTGLT"
     gene            3947..7042
                     /locus_tag="VFH05_08305"
     CDS             3947..7042
                     /locus_tag="VFH05_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HET6678256.1"
                     /translation="MNISAPFIRRPVATTLLTIGVTLVGIIAFRFLPVAALPQVEFPT
                     IGVQANLPGASPETMASSVAAPLERQFTRIAGVTEMTSSSTLGSTGITLQFELNRDID
                     GAARDVQAAINAARADLPANLPSNPGYWKNNPADAPILLLSLTSDIVDRGVMYDAASS
                     ILQQKLSQIEGVGRVYVGGGSLPAIRVDLNPTALNKYGIGFGDVRRMLSATNVRRPTG
                     HLTDGTQTWEIRTNDQLHKADDYLPLVVAYRDGRAVRLSDIATVETSVEDVRNMGMAN
                     MTPAVLVMVYRQPGANMIETVDRIRAVLPQLEASIPGTIKLSLFSDRTGVTRASLHEV
                     ERTLAISVLLVILVVFVFLRDVRASLIPSVAVPVSLISTFGVMYLLGYSLDNLSLMAL
                     TIATGFVVDDAIVVLENISRYREQGMPMLQAALRGAQEITFTVLSMTLSLVAVFLPIL
                     LMGGIMGRMFREFAVTLSVAILVSLVVSLTTTPVMCAMLLKPHRPGPHGRWYRISEGI
                     FEGMRGGYAGTLGWVLRHPRIMLGLTLATMGLSGYLYTVVPKGFFPQQDTGRISGRIQ
                     AAEDISFQAMREKLTEIVEIIRSDPAVESVTAFSGGGTNNVGRSMVSLKPLQERKITT
                     DEVIARLRPKLAKIPGASTYLQTIQDLRIGGRASSAQYQYTLQSVDLAELNTWAPRVE
                     QQLRTLPEIADVNSDQQDKGLQSLVVFDRSTASRLGISPQLIDDTLYDAFGQRLVSVM
                     YRPLNQYHVVMEAAPQYWQNPATLHDIYVRSPSGAQVPLSAVARYEPTSTLLAVNHQG
                     QFPAVTLSFNMVTGISLGQAVAAIERELQQMALPAGIRGSFQGAARAFQSSSDNQIWL
                     ILGALLTVYIVLGILYESYIHPITILSTLPSAGVGALLALLLFKTELSVIAMVAIILL
                     IGIVKKNAILMIDFALEAERKEGKGPAEAIYEACLLRFRPIMMTTMAALFGALPLALG
                     TGVGSELRRPLGIAIVGGLLVSQLLTLYTTPVVYLYLDRMRLRFLGRMHRSIEPRPLP
                     Q"
     gene            7138..7692
                     /locus_tag="VFH05_08310"
     CDS             7138..7692
                     /locus_tag="VFH05_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678257.1"
                     /translation="MMSRLSTFQWVSTVFLAVTVLTGCPRVLYLDYHPSASITGSGPV
                     RVDTFTYAGHPTGLMKQKEVESGDYDPERLFLSQDIGEFFADALRKELTSGGYEARPD
                     SIRAVSGTIEQFFLDYVGGQEQRFQIQTLFQVSREGGPAFTTSCRSERQQAKDWMKSG
                     SLIEHGVRDCIEAFVKEAQAAGAL"
     gene            7714..8898
                     /locus_tag="VFH05_08315"
     CDS             7714..8898
                     /locus_tag="VFH05_08315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6678258.1"
                     /translation="MPGFEYIGEAEHQQVREVMASGVLMRYGFDGPRKGQWKCRELEE
                     NLATRFGVRRAHVCSSGTSALSTALAVCGVGAGDEVVLPPFTFVADLETVLLAGAVPV
                     FADIDETLCLDPRSVAAAMTPKTKAVLVVHMCGAMARIDAIMELCRTHQVTLIEDAAQ
                     AAGATFHGKALGTFGRVGCLSFDYVKTITCGEGGAVLTDDEETYRLAQAYTDHGHDHA
                     GADRGADGHPHIGANFRLSELHAAVGVAQLARLDDILEVQRRHKAMLKDGLAGIRGLQ
                     FRELPDPAGDSATFLSLLLPSEHDARQAAKALPEAGVDGCFYWYDNNWHYHRKWDHIK
                     TFTSPGRLAIDRFGYGSDLTKITLPQSDAVIGRTISMLIKLRWTEEHVRDRLARMKSV
                     LA"
     gene            8931..9689
                     /gene="kdsB"
                     /locus_tag="VFH05_08320"
     CDS             8931..9689
                     /gene="kdsB"
                     /locus_tag="VFH05_08320"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET6678259.1"
                     /translation="MNVAIIVPARIGSTRFPKKLLHRIKDKPVIVWTADRIKKEAPDL
                     PLYFAVDHESLADVLRRDGYEAIMTDPAHTCGTDRIAEANRRLKARYVINVQADEPLV
                     TGGQIRQLVDLIQADTEMATLGTPVRYDKDYRNPNHVKLVCDEKGYAVYFSRAPIPYL
                     RDTHGAFDAELAASIPVLIHLGLYAYTASFLELFGTLAPGKLEQVEKLEMLRAMERGH
                     RIKVGVTKEALVEIDTPEQAVEFEQVVTERFPAH"
     gene            9730..10803
                     /locus_tag="VFH05_08325"
     CDS             9730..10803
                     /locus_tag="VFH05_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HET6678260.1"
                     /translation="MYRNLRLVPQLIYGRGSINQLGDILTKQRTDAGSPVVFLIDAVH
                     EKRAFIQKLPLRANDLVILVDVGTEPKTQYVDELTERIKAFSNRQPDAIIGIGGGSAM
                     DLAKSVSIMLTNPGSAADYQGWDLVKYPAIYHLGIPTLAGSGAEVSRTAVLTGPVKKL
                     GINSDFTPFDQIVMDPDLIATVPPNQRFFTAMDCYIHSCESLQGTFINEFSRAFAESG
                     RDICRDVFLGRCEDVVADERLMVASYFGGLSIAYSQVGICHALSYGLSYVLGVRHGVG
                     NCIVFNQLEEYYGQDVREFQQMIRQNRIDLPSGVTNGIDEASMNRMIDTALGLEPLWK
                     NALGDDWKQRISREKIRSLYVRM"
     gene            10863..11942
                     /locus_tag="VFH05_08330"
     CDS             10863..11942
                     /locus_tag="VFH05_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6678261.1"
                     /translation="MNILIVKTSAIGDVIHTLPSLWSLRAHFPDAQITWLVEESAADL
                     LYGHPALNRVLVARRKTWLNDARAGRPSRAFRGVLQFIRQLRDTRYDLIIDFQGLIKS
                     AIWVALAKGGRKVGFGRGMEHAEHSYLVLNERLPAVDMNHHAIDRSLLLLKGIGVPAA
                     DLRYTIPVSAAQESEAGALLLTVGVREGDRLVAINPMGRWPTKLWEPASFAALADRLQ
                     REGLSIVFTGGPHDHTALDEICRLMTSRCRRLDGKTSLNTLAAIYRRAEVLVTTDSGP
                     MHLAAAVGTSVVALFGPTAPWRTGPYGANHVVLRADVSCSPCFKKQCSTTVYEERACM
                     KRLTVEDVTRAVLEKISAPPLAASK"
     gene            11858..13261
                     /locus_tag="VFH05_08335"
     CDS             11858..13261
                     /locus_tag="VFH05_08335"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HET6678262.1"
                     /translation="MHEALNRGGRHARSAGKNFSPAARRLEVTPHHPHINPHQWIILT
                     LYRALFTAGVILFVICFPLWIFNSKRQQTLFKRLGWQQYPRLQASRRSPVWVHAVSIG
                     ELLSAGSLIESLRRETGGERPLYLSVSTLSAFALATERFGRSCDGCFYFPYDVRFAVK
                     RCLEKINPALVVFIETDIWPGFLADVRRNAVPCLLVNGRLSPESFRTYRTVSALFKPA
                     FNTFRSIYPQSQGEADRFLALGIEPGRLRTPGNLKFDAAPPIPDDALLAALRKEFHIL
                     PDSPILIAGSTHSGEEEILHSCFLRLRRKFPALRLILVPRNPGRGSEIVELFQGDGVH
                     AALFSRLDRSTPVVVVDRLGYLSRLYALADLAVIGGSFVPRGGQNPIEPAACGKPVLF
                     GPDMHDFPDVSRWLLEAGGAIRANNEEELYAACDRLLTDPEKAAEMGRHGRRVVTAHQ
                     GTTARIVQDIVSILSAK"
     gene            complement(13278..13892)
                     /locus_tag="VFH05_08340"
     CDS             complement(13278..13892)
                     /locus_tag="VFH05_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678263.1"
                     /translation="MRGVGPVAAVCLCVLAGCAAKGERIDVAIPSTSPNQATTNSGSM
                     GPRVVVKPFDDVRLDRTHLGSRSHLWGNTSYFDVPNGTVGEAVAKALVQQLSKRGWQA
                     SLDGSNGTAPDATISGTIQDLSVQAVSKVGHTQLEAKNTMMVRVANHGDESSIQERVM
                     SSGSDEVFWFEPEDAQQLVNEVLEKNIEKFIADTKLDGRALRLR"
     gene            complement(13956..14606)
                     /gene="pal"
                     /locus_tag="VFH05_08345"
     CDS             complement(13956..14606)
                     /gene="pal"
                     /locus_tag="VFH05_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET6678264.1"
                     /translation="MPEREIRHTILAAVSCVIILTTFSGCGKRVAGSSGDASSTRKST
                     AAPVETIQPEGFATIPDDKSSFEAMRTPGDMASAPSETSPPSAPGEPAPAPAPSSAAS
                     SPLDASNLSDIFFDYDQYAIRRDAQTILGANADWIKNRPGKAVVIEGHCDERGTQAYN
                     LVLGDKRARSVKRYLEDLGVPTSRMKTTSYGEIRPFCKERDESCYQQNRRAHFVVQ"
     gene            complement(14794..15438)
                     /locus_tag="VFH05_08350"
     CDS             complement(14794..15438)
                     /locus_tag="VFH05_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678265.1"
                     /translation="MVAQGNGARGLLAALLWVGFTPALAIDVKLSSEEAQKALEAGRT
                     PMEKANSPEEVRKVLQHASMVTRVGADPEKDACGASAILRTKRYRLEAFGRQEASESK
                     KRKTDIRMPDEFIRKVVDMPNMEVEVQLCGDDEYFAEGALIELQQGSKKVKAIDVGKA
                     ERGRKNEGSGPAYRSRFTAVFAYESFDPNAASVFVINLQDGKEVRIPADFSKVK"
     gene            complement(15499..16134)
                     /locus_tag="VFH05_08355"
     CDS             complement(15499..16134)
                     /locus_tag="VFH05_08355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET6678266.1"
                     /translation="MWRLVLASTSPRRRDLLALLGIPFDIRDPVYEERLVSDRSADEL
                     VRAFAQCKADSVAQRDPDALVLASDTLIEVDGEVLGKPGDLVEARKILRHLAGREHRV
                     KTAVTIACRGRHLEATDLSTACIRMKPFDAEAHEHYLTTGDSLGKAGAYSIQGPGADL
                     IESLEGDFTTVVGLPLRLVVRMLQESGMTVPVDVDMVYDTKPYSNWARFSG"
     gene            complement(16122..17297)
                     /locus_tag="VFH05_08360"
     CDS             complement(16122..17297)
                     /locus_tag="VFH05_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HET6678267.1"
                     /translation="MTFWNTLPMPIIGLSPMDGVTDSAFRHTIASQGKPDVIFTEFTH
                     VHDVCRGPEFLLDSLHYDESERPIVAQLYGKDPELFYQAAHVVCELGFDGLDINMGCP
                     SRNVASSGSGAGLIRTPDLAHAIIRAARQGIADWTAGQTLEGAGLKPVRVDMVNRLNG
                     QRDVAATRARLPVPLSVKTRLGYDEVIVERWIEHLLSESPAVISLHGRTLQQMYRGEA
                     DWSAIARAVQVARGSGTLLLGNGDVQNLSDVVRRVSETGVDGVLVGRAALGAPWFFRD
                     KEQARRCVQTDRPLAVAPWTPDLTARFRVLLEHARVFERQWGSRQFRRMRKHLGWYCK
                     GFPRAAALRARMFLVSSVAELEAVLADYQRFVHAYDQEETSAGLLESAAPLTSRCGG"
     gene            complement(17294..18784)
                     /locus_tag="VFH05_08365"
     CDS             complement(17294..18784)
                     /locus_tag="VFH05_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HET6678268.1"
                     /translation="MRGERSVNQVFQCRAEEIQQQGFGLSVDVYSPNLFELMSRFERA
                     STRPAYLEIFRAAAAALETVTRRMTGVSFAYHGEGLWITQPDFARAPHLRDELDDVIS
                     QLHILASPWLNHECATKQMAGYSFGTYLPPLYTAESARVVADNIEIVQAKMDERRSPG
                     QNFGPLFLLEMPPLTYFMAGTLAVPTYFRLVADLTSCGFVLDIGHLWTVYRYSPACRE
                     MNLEAFVERFLNEFPLERVIEVHVAGLAPHEASVSRGHSDRGHPEWIDAHVAPVPDVS
                     WMVLEQVLAHPRLCNLRGIALEVDNKPMEQIVNEFQYAHRRFSSAIDRCLSNHLLAPD
                     TRGPANEGGTAPVACEVDREQLVAEYAQYAQIVSGRQPPTGPNWEEVAVDTVGLSRYI
                     HEYLPHEILHWGGDITDMFPETSRRLNETGLPLSQFVTWWFHTPRPVDQPYDFFLLKI
                     DLLVEFVSERAPALLSLTRQEADALRSAYIDANAHADLEAVAESQR"
     gene            18959..19732
                     /locus_tag="VFH05_08370"
     CDS             18959..19732
                     /locus_tag="VFH05_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6678269.1"
                     /translation="MLGQNEPSPQAHHPTPIVVAHMMTPGVVQVPGDVSVSEAALLLE
                     REHAPCLLVKETDTVFGLMTPHDIVKKVVAQGLEPHDIEVRSIMTRPVQFIEYDELLE
                     EASTMMVSTGASLLIVTKENQPVGVLSAQDLMLTPARRLMNIPTIVTVVDEAVPQRQA
                     HNATIVQLSHVSTRIESASSLHPKTRVVLAFSLPGQHTPLSIHGKVLSSETLPATATH
                     ESAERTVAVEIQFADLSATDLARLKAWTLQAIPSSLDRP"
     gene            19821..20864
                     /locus_tag="VFH05_08375"
     CDS             19821..20864
                     /locus_tag="VFH05_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET6678270.1"
                     /translation="MKTTPSRSRTTLSNDEILRQIRSLLECPEDDLSAGLLKELMAGI
                     IKLHDAHLEPLDLKIVNRAIKELRHAFRVFRDYHHRKKVSIFGSARTTADDPNYRIAF
                     DFCRRVVEAGFMVITGGADGIMRAAQEGAGRENSFGVNIMLPFEQGPNNTIADDPKLV
                     TFKYFFTRKLIFQKEANAIALFPGGFGTHDEGFEILTLAQTGKSEPQPIICLQAAGCD
                     YWDDWYGFIKRQMLDRRLISPEDLGLFKITSSVQEAIDEIRTFYTRFHSIRYVGRQLA
                     MRLTGPLSAEQVAAIHEQFADLLLEGTFELRGALPEELDEPDLKDLPRLVFASNRRSA
                     ARLRQLINHVNRL"
     gene            20976..21851
                     /locus_tag="VFH05_08380"
     CDS             20976..21851
                     /locus_tag="VFH05_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET6678271.1"
                     /translation="MGQFDSRQHFPLAPTVVLYHAECADGFGAAWALWRRFADARFVP
                     VKHGSPPPDGLQGQRVVIADFSYGRETLEMMAGETDRLLILDHHITAEKALAGLPYAY
                     FDMKKSGAVLAWEWAHDHPVPWLLDYIQDKDLWTWALPMSREINAAVASHPFDFQLWS
                     SFKQKDLEHEGRAILRYEHELVNKLSGQAVLVEFQGETVPSVQSAILTSQIGERLSAE
                     APFCIIWHDRDGRRYYSMRSREDGTDVGAIAASFGGGGHTHAAGFSVPLGPDGSLPDN
                     PALPRSIVDRRRNST"
     gene            21860..22567
                     /locus_tag="VFH05_08385"
     CDS             21860..22567
                     /locus_tag="VFH05_08385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6678272.1"
                     /translation="MIPLQDDNPSERSPVVTIGFIAICVFVFFYQASLPPQPGEMFVF
                     QYGAIPAILFGQADLPPAIAVGIPAYATVITSMFLHGGWMHLIGNMLYLWIFGDNVED
                     VMGHVRFVVFYLTCGILAALSHALTDPTSTIPMVGASGAISGVLGAYLLLFPHAHVLV
                     FLPGIGMTRVAAGIVLGMWFVMQLLSGGMSIGGPGGGVAFFAHIGGFVAGMALIGFFK
                     RPEVRFFSPAKTRRWDL"
     gene            complement(22603..24447)
                     /locus_tag="VFH05_08390"
     CDS             complement(22603..24447)
                     /locus_tag="VFH05_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6678273.1"
                     /translation="MHGTLAFSPDSISSPETDEQGAVSRPRYVILQLLVAIMLGYQLL
                     FGPELVVSRAASEFIVGGLVVLIGCLLAVPSSVLQAAWFSGTLIAVDTALVTGTMYLS
                     GQARSELYLSYFILMLIAASLRRLSHVIAFSLLLCAGYGFVLYQGSVQTGVLSVGQLL
                     GVPVLLVMAIFYGMALETIGAERQQKTQLLHNIDALKKTEQALEASRDQLEIRIKGLK
                     EDLSKANALVQQEKSERQGLERQLVEAQKMEAIGRMTSGLANEFSHLFSVIGKQTGVV
                     LSHLLPEDPLYGAVDDIFRTGERAAALTAQLAALDIQHRQFRHVLSMRTLMEDLSETI
                     RDLLPSRIDCQISAEGSHLDVEVDREGIEQMLLNLTLNARDAMTGGGRLTISISSCEG
                     GADVKALAGVNRCASAVRIQVCDTGSGMTRDTQSRMFEPFFSTKETNVGLGLTSVYGV
                     VKQHGGQVEVASQPGHGTTVRIYLPAVEAGMVPNEPAEFPVAAKGQETILLVEPHELE
                     RKLAVSTLKRHRYHVLEASSPVEAIMLAHQYSGAVHVTVSGLIMPEISGRELARRLLK
                     QHPTMKALFVSGYDDAAIISHRINRRFLLRRPYRQRGLVEKVRELIDA"
     gene            complement(24627..25799)
                     /locus_tag="VFH05_08395"
     CDS             complement(24627..25799)
                     /locus_tag="VFH05_08395"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET6678274.1"
                     /translation="MNGPLVEPGSVLGVLGGGQLGAMFAQAATRLDYRVAVWDPDPDA
                     PAHHLAAYSFPSPFTDQKTFAEFAGLVAVVTYEWENVPAGLCERLERTTPVRPASSVL
                     RRIQNRLVQKTFLAGQGFPVPRFAALTEPERLAEVLEQLGYPAICKTATAGYDGKGQW
                     KIDEPSSVFSVRDSLTASCHSTMEWIVESFVPFERELSVLVARGIDGQTIVYPLVENV
                     HEAGILRMTVVPAEVPSNVVERSADLARAVVARMDGIGLFCLELFLLPDQRVLINEVA
                     PRPHNSGHYTLDACNVSQFEQQVRAVTGLPPEEVLLTNPAAMVNLIGEDAETVKRDVE
                     CRRLLGLPGAYVHDYGKRAIRPRRKMGHVTFLAHDVQTARDRASRLRQSLLQASRN"
     gene            complement(25796..26374)
                     /gene="purE"
                     /locus_tag="VFH05_08400"
     CDS             complement(25796..26374)
                     /gene="purE"
                     /locus_tag="VFH05_08400"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET6678275.1"
                     /translation="MPETSKRGGSPRTKRGGSRPIVGVLGGSKSDFPILEKAVAILNE
                     LGIANELIVVSAHRTPDRLFAYAEQAHARGIEVIIAGAGGAAHLPGMLAAKTHLPVIG
                     IPIPTEHLRGLDSLLSIVQMPRGIPVATVAIGGAENAGLLAAQILAGRYPEVAVRVKS
                     FRHAQTTHVLQSPEAKGSVADFKPSLHQPRRL"
     gene            26553..27989
                     /locus_tag="VFH05_08405"
     CDS             26553..27989
                     /locus_tag="VFH05_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET6678276.1"
                     /translation="MAGIGHSLRERVRLLVQRPTGLDEIAVDDFAASSKLRAWEAVQI
                     PERLRFSSRLAIKLVLLFLLIIAFVPWTQTITVSGQLSAYTPYERPQDIEAQITGRIK
                     KWHIFEGVRVKQGDLILELDDYDPNFMSPDLLPLLEQRKKALTETRQAALARAEQLDK
                     RIKEMQNLVKAAVPSAQARVVEAENQVREAYQKVEAAKIAVATAELNVDRHKQLAEQG
                     LVSQRELEVTIQAAIATKADLTGAQAHLRATEQAMKALSFGREQINAEVLQRLLDAEA
                     ARDAAVGEAAKATDQLADVSLRMSNAEQRRVAGRIMAPIDGTVVKMAETGIGETVRPG
                     DKLVKISPASADKAIEMVADGIDAPLLNVGRKVKILFYGIPAIPLPAWPELMAGTYGG
                     VIKVIDQVDDGKGNFRFWVVPDAEEKPWPPQEHVRQGTKVMGWVILNRVPLWYELWRR
                     FNLFPPDYQERPPSLIDTLLPKAGRGAK"
     gene            28109..29590
                     /locus_tag="VFH05_08410"
     CDS             28109..29590
                     /locus_tag="VFH05_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6678277.1"
                     /translation="MPHPYVLAIGLLIALGIPWSYASAADPSTKRELPPIPLSVDEIH
                     AWVDRSHPLLKGAGTEKVMARGRMLKALGAFEPNVVNDTELERFISSSNPNAGTQTAG
                     FNDTLVEARHPLGFRYSAGIRQAIGDARIPDLGFNNDRQQVLLGGFFPLLRGLMVNPE
                     NAELQRSELADPRADVRIAQTRQDLFLAAATQFWDWVAAVKFLEVQRRAVGIAEDRFK
                     QVEGRAKAGAVAPLDVVEANQEVQRRREVSIAAQRFVEQEQLKLSMFLWENNAPVNPP
                     LERAPEFPSQMLIPTPETIKAHKIQAKADRPEIKEIGIEAKINNIDLELAKNNLLPSL
                     DAEAAPARQPEKFVLGLGYRFGLELRIPILQRRSRGEVLEAQGKADRFVLTQKFREQQ
                     VVIDVDNALSAIERAKERIAAAVESLRLAKTLEEGERFRFSLGATSVLFVNLRERNSV
                     DSEGQVIRAKADYQKALALYQWSIGAWAKALPSSIPVNYRARD"
     gene            29688..31349
                     /locus_tag="VFH05_08415"
     CDS             29688..31349
                     /locus_tag="VFH05_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6678278.1"
                     /translation="MQSHVDDQPNLFQSVVGYVGVLLRLERRLLALVVSYSLAIGLFA
                     LIVPLTVQELTNTFAFAIQPVTIVTLAGVMIAALLFVGAFRSLQYYAVEVLERRLFAR
                     VAIGMAQQIPQFQFLGFKPRYANHFMETVFMQRALSVLLVDLINVVVGGAVGMTILVF
                     YHPYFLLYNALLLAGFSIVFLLSHGGLKATIEMSHAKYDTLHWMQEISYNLLHFKATD
                     SQAILMQRTDELVGKYVQTRQTRFGILIRQYLGSVGWQAVAHGGLIATAAWLLSIGQL
                     TLGQLVAAEVVVSGLLASFDEVVKRMGHIFYFMTGLRELDLVLSLPKDQGSSALSVPL
                     PDPTVHGIRLTCKDLTVHHAGVPAIFEQFNVEVTPGEKIGIYASTTAAKTALARVLAG
                     LEMPTGGVIRYNGVDLRHIDMNAINRCRGFMIDSQLTLFEGTIEDNILLGRSYIPYSD
                     VRWALRFAELEEDVDTLPQGLKTHIRAPGKILAPTHMMRILLARAILGRPQILIFDGI
                     LHTLQPVLRETLLRRLCSKDEPWSVIFVSNDPNLTPHVDRRLILE"
     gene            31360..32145
                     /locus_tag="VFH05_08420"
     CDS             31360..32145
                     /locus_tag="VFH05_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HET6678279.1"
                     /translation="MLLLRWLRSFRPSASQILISLLIAGLGWLSGGALSRVDQDLRIM
                     YTEYTLGAADLAHISADVMRYRNTIIRALEAGSRKDFERITESLPGQRARVQLAVDRY
                     AAAGLRVSRSGRSEPEDIEAVRKSLDQYFSAASTTIDLLSREWNASSPSERDRLRRNA
                     EEHAADNAGPKMIQVSLALDRLLDTVADVAKDMRDEGTKAIQSTSVMLVVGSFFIAVL
                     NLFLTRPRTPRGQEAGRSPEPLSEPLRPTLPLRPGNQQHPIRS"
     gene            complement(32142..32876)
                     /locus_tag="VFH05_08425"
     CDS             complement(32142..32876)
                     /locus_tag="VFH05_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6678280.1"
                     /translation="MDKIRINKFFTEHGICSRREADRLIEAGRVTVNATVAKLGDQVS
                     TADVIACDGRVIPWGQTRVYIKYHKPVGVTTTSESHVPRNIISEIGHPERIFPIGRLD
                     KDSSGLILLTNDGDIVNEILRTEFGHEREYEVGLDRPYDQSFLDRLAKGVVILGKKTQ
                     PCRVQRLSPKRFTIILTEGRNRQIRRMCQALGYRVVALHRVRIMHVTLAGLSSGRWKP
                     LTTEEQQELFRAVRRSSASHEDAEHS"
     gene            complement(33024..33956)
                     /gene="speB"
                     /locus_tag="VFH05_08430"
     CDS             complement(33024..33956)
                     /gene="speB"
                     /locus_tag="VFH05_08430"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET6678281.1"
                     /translation="MTLPAGWEGSQHNFLGLEEPWCHPDQAGVYVLPAPYEHTSSYIL
                     GSDEGPSAIIEASHQVECYDETLRCEPFREWGGIATVAPLELKGKIDREAVDAIEQFV
                     QSHIGNGRFLVTLTGEHTGALGAIRAHARRYPNMSVVQIDAHGDLRHAYQDNLYSHAS
                     VMARVVDDGLPLVQVGIRSICPEEIERIRRTDRIATFFAAELLDPSGPYEGKASRWIP
                     QVVKACQGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDTLNLITALANGPGILGMDI
                     SEIAPIEGFVAPQFCVARLIYRILGRIKAGRRVH"
     gene            complement(34017..>34489)
                     /locus_tag="VFH05_08435"
     CDS             complement(34017..>34489)
                     /locus_tag="VFH05_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HET6678282.1"
                     /translation="CNLVSVSSILPPNCKIIPRKRGEKLLNPGEITHCVMARSETNER
                     NRLISASIGLAIPTDRRTYGYLSEHHAHGETDEETGEYTEDLAAQMLATTLGVEFDPN
                     IAWKEREQVFKMGGKIVRTLNITQSAIGKPGRWTTVVALAVFIPLENLPKRARR"
BASE COUNT         7096 a        10324 c         9810 g         7259 t
ORIGIN      
        1 cagttaaaaa atgcgcgtct ggacttcgaa cgatataagg gactctacga acagggcttt
       61 gttccgaagc agcagttgga tactcaggaa gccatcgtcc gtcagtcgga aggcatcgtc
      121 aaagccgatc aaggccaaat cgacaacgcg aagcttcagt tggcctatag ccgcatcacc
      181 gctcccatta gcggccgcgt cggcttgcgg caggtggatc ccggcaatat ggtccgcgcc
      241 aacgacacca atggccttct cgtcattaca caactcacac ccattgcggt gattttcacg
      301 attcctgagg atagtctgcc ggccgtgctc gaacggctca acgccggtca gcaactgacc
      361 gtagacgcat tcgatcgtga gcacaagaaa caactggcca ccggcaccct gctgacggtc
      421 gacaaccaga ttgatcccaa tacaggaacc gtgcgactca aagccgtctt cccgaacgaa
      481 gacggcgctc tatttcccaa ccagttcgtc aatgcgcgcc tgctgttgga cgtcaaacgt
      541 ggaacgacgc tcgtcccgtc tgcagccgtc caacgaggtc cccagggcac gttcgttttt
      601 gtggtgaagg gagaccacac ggtcggcgtg cgggcgatta cggtcggcgt cactcatgga
      661 gaagaatcgt ccgttgaatc gggtctggtt ccggacgacc tcgtggtcgt ggacggggca
      721 gaaaagcttc gagatggaac caaagtcgaa atccgggcgc cgcgcgacgc acctccgccc
      781 gctaccgggc tgccgccgac cgtaggggac gagccgcgta aaggaagccg ctcgtgaacc
      841 catcccgcct ctttattctg cgtccggtag cgacaaccct gtcaatggcc gccatcttcc
      901 ttgccggatt catcgcgtac aggttgttgc cggtatctgc gctaccgcaa gtcgattatc
      961 cgaccattca ggtggccaca ttctatcccg gtgcgagtcc cgatgtgatg gcttcgtccg
     1021 tcaccgcgcc tcttgaacgg tatttcgggc agatgccggg attgactcag atgacctcca
     1081 caagttccag cggcagctcg gtggtgacgt tgcaattcag tctcgacctc gacctggatg
     1141 tcgccgaaca gggcgtacag gccggcatca acgcggcctc tacatttctg ccacgggatc
     1201 ttcccagccc gccggtctac agcaaggtca acccggccga cgcgccgatt ctgacactgg
     1261 cgctgacgtc ggagacgctg cctctatcgg aggtggagga ttttgccgaa accaggtttg
     1321 ctcagaaaat ttcgcaaatg tccggcgtgg gtcgtgtgac catcagcggc ggacagcgac
     1381 cggcggtgcg catccaagcc aacccgacga cgttggcagc ctacgggctg actctcgaag
     1441 acctgcgcct cgccctggcg gccgccaacg tgaaccaagc gaaaggcgca ttcgacggcc
     1501 cccggcgggc ctcgatcatc aacgcgacgg atcaactcat ggtcagtgcg gactaccgcc
     1561 cgctgatcgt gtcgtatcgc aacggggccc cggtgcatct gtccgacgtc gcggacgtca
     1621 tcgatgatgt cgagaatgtc aaacaggcag cctggatgaa cgacgtcccg gcggtcatcg
     1681 tcaacattca gcgtcaacct ggcgcaaacg tcatcgaggt ggcagaccgc atcaaaactt
     1741 tacttccgca gcttcaaagt gcattgccat cgtccgtgca tgtgaccgtg ctgaccgacc
     1801 gcacgacatc aatcagggcg tcggttcggg acgtgcagtt cgaattgatg ttggccgtcg
     1861 ccctcgtgat cctggtgatc tttctgtttc tccgaactct ttccgcgaca gtcattcctg
     1921 cggccgccgt tccgctctcc ctcgtcggga cgttcgggct catgtacctg atggggttca
     1981 gcctgaacaa cctcacgctg atggccctca ccatttccac gggttttgtc gtggacgatg
     2041 cgatcgtgat gatcgagaac atcgcacggt acatcgagaa gggggatacg ccgtttcagg
     2101 ccgcactcaa gggttcccaa cagatcgctt tcaccgtgct ctcattgacc atctcgctca
     2161 ttgccgtgct gattccgctg ctgttcatgg gggacgtcgt cgggaggttg ttccgcgagt
     2221 ttgccattac cctgagcgtc acaatcctca tctcggccgt ggtgtcgtta acactgaccc
     2281 cgatgatgtg cgccagactc ctacgacaca gacctcagac cgaacagggt gcgttttacc
     2341 gacagaccca gcggctgctc gatcgggtca ttgcgggcta tggacggtcg ctacgatggg
     2401 tgctgaatca tcagcaggcc acgctggccg tagcggtagg cacgctgatg ctgaccgtcc
     2461 tgctctacgt cgtgatcccc aagggctttt tccccgttca ggataccggt gtaatcctgg
     2521 gcattaccga agcaccgcag acgatttcat tcggcgccat gcgggatcgg cagcgggccg
     2581 tggccagggc ggtcctcgag gatccggccg tcgagagcct atcctcgttt attggcgttg
     2641 atggtatcaa caccaccctc aacagtgggc ggattcaaat caatctcaaa cctttcaaag
     2701 agagaaagct gagcgcaagc gacatcgtcc agcgcctaca gtcccaggtt gccggcgttg
     2761 gagggatcac gttgttttta cagccggtgc aggacctcac agtcgagaat cgcgtgagcc
     2821 gcacgcaata ccaatacacg cttgaagatg cggacccgga ggaactgaac cggtgggcgc
     2881 ccgccttagt cgagtcattg caggcgctcc ccgagttgcg cgacgtgagc agtgatcagc
     2941 agaaccatgg cttagccgcc atggtgaata tcgatcgaaa tacggccttc cgcctgggga
     3001 tcactcccca gctgattacc gatgccctct atgacgcatt cggtcagcgg caggtctcca
     3061 ccatgttcac gcagctcaat cagtatcgag tcattctcga agtgatgccg gagttccaaa
     3121 aaggaccgca ggctatgcaa ttcatcggcg tgcgctcgtt cggcggcgga caggttccgt
     3181 taaacgtctt caccaccgtg acggaaacca cggcgccgct tacggtcaat cgtcaagcgc
     3241 aattcccctc cgtcacgctg tctttcaatc tcgcgccaag aacgtcgctc ggtgaagcgg
     3301 tcaaagcgat cgagcgggtc acgcaaaagg tcggcatgcc gcctagcatc cgcggcagtt
     3361 tccaaggtac ggcccaggcc ttccgtgctt cactggcgaa cgaaccactg ctcattctgg
     3421 cagcgctgat gacggtgtac atcgtgctgg gcgtcctcta tgaaagctac atccatccgc
     3481 tcacgatcct ctcaacgctg ccgtcagcgg gcgtcggtgc gctactggcg ctcatgctgt
     3541 ctggcaccga attcagcgtg atcgccctca tcggcatcat tttgttgatc ggcatcgtca
     3601 aaaaaaatgc catcatgatg atcgattttg cgctcgatgc ggagcgtaag gacgggaaac
     3661 accccaccga agcgatctac gaggcttgtc ttctccgttt tcgtcccatc atgatgacga
     3721 cgatggctgc cttactgggc gcccttcctc tggcgatcgg gtcaggcgtc gggtccgagc
     3781 tgcggcatcc tttgggtatt gccatcgtcg gcggtctcat cgtgagccag gtattgacgc
     3841 tttacaccac gccggtcgtc tatctcgcgt tcgatcgctt ggctcgacgc gttcgcgccg
     3901 gaacatctca gacgccgatt tcagaagcgg tcggcaccgg gctgacatga acatctccgc
     3961 accattcatc cgacgtccag tcgctacgac gttgctcacg attggcgtca cgctcgtcgg
     4021 aatcatcgcc tttcgatttc tgccggttgc tgcccttccg caagtcgaat tccccacgat
     4081 cggcgtccag gcgaacctgc ccggagcaag tccggaaaca atggcgagct cggtcgcggc
     4141 accgctggaa cgtcagttca cccggatagc cggcgtgacg gaaatgacct catcgagcac
     4201 actcggctcg accggcatca cgttgcagtt cgaactgaac cgcgacatcg acggagctgc
     4261 ccgtgatgtc caggccgcga tcaatgccgc ccgcgccgat ctgccggcca accttccgtc
     4321 aaatcctggt tactggaaga acaatccggc cgatgcgccc attctccttc tctcgctcac
     4381 ctccgacatc gtggaccgcg gtgtgatgta cgacgccgct tcgagcattt tgcagcaaaa
     4441 gctctcccag attgagggtg tcggcagggt gtatgtggga ggaggatctc ttccggccat
     4501 tcgcgtggat ctcaatccca cggcgctgaa caagtacggc atcggattcg gcgacgtccg
     4561 tcgcatgctg agcgccacaa acgtgaggcg gccgaccggg cacctgactg acgggacgca
     4621 aacctgggaa attcggacca atgatcagct ccacaaggcg gacgactacc tacccctcgt
     4681 cgtcgcctat cgggatggac gggcggtgcg attgtcggac attgcgacgg tggagacgtc
     4741 ggttgaagac gtgcggaata tgggcatggc caacatgacg cccgctgtac ttgtgatggt
     4801 ctatcggcag cctggtgcga acatgatcga gacggttgat cgcattcgcg cggtgttgcc
     4861 tcaactggag gcctcaattc caggcaccat caagctgtcc ctcttcagcg accggacagg
     4921 agtcacccgg gcgtcgctgc acgaggtcga aaggacactg gctatctcgg tcctgctcgt
     4981 gattttggtc gtgtttgtct ttctccgtga cgtgcgtgcg agtcttattc cctctgtggc
     5041 ggtgccggtg tcgctcatca gcacctttgg cgtgatgtac cttcttggat acagtctcga
     5101 caacctctcg ctcatggcgc ttacgatcgc aacagggttc gtcgtcgacg atgcgattgt
     5161 cgtgctggag aacatcagcc ggtaccggga gcagggaatg cccatgttgc aggccgcgct
     5221 gcgcggtgcg caagagatca cgtttacggt gctgtcgatg acactgtcgc tcgtcgccgt
     5281 gtttcttccc attttgctca tgggaggcat tatgggccgg atgttccgcg agtttgccgt
     5341 cacgctctca gtggcgatac tcgtctcgct cgttgtgtcg ctgacgacaa cgcccgtgat
     5401 gtgcgcgatg ttattgaagc cgcaccgccc cggaccccac ggaaggtggt atcggatcag
     5461 cgaagggatc tttgagggca tgcggggagg atatgccggg accctggggt gggtactgcg
     5521 ccatccccgc atcatgctcg ggctcacatt ggcgaccatg gggctcagcg gctaccttta
     5581 cacagtcgtg ccaaagggtt ttttccccca gcaggacacc ggccggattt ccggccggat
     5641 ccaggctgcg gaagacattt cttttcaggc gatgcgcgag aaacttaccg agattgtgga
     5701 gattatccga agtgatccgg ccgtggaatc cgtaacagcc ttcagcggcg gagggacaaa
     5761 caacgtcggc cggtcaatgg tttcattgaa acccctccaa gagcgcaaga tcaccacgga
     5821 cgaggtgatc gccagactgc gccccaaact ggccaagatc ccgggcgctt cgacatatct
     5881 gcagaccatc caggatttga gaatcggagg ccgggcgagc agcgcccagt atcaatacac
     5941 gttacaaagc gtggacctcg ctgagctcaa tacctgggcc ccgcgcgttg agcaacaact
     6001 tcgcacactt ccagagatcg ccgacgtcaa cagcgaccaa caggacaagg gcctgcaatc
     6061 cctggtggtg ttcgatcgca gcaccgcctc ccggctcggc atcagccccc agctcattga
     6121 cgacacgctg tacgacgcgt tcggtcagcg gctggtttca gtcatgtaca gacccttgaa
     6181 ccagtaccac gtcgtgatgg aggccgcccc tcagtactgg cagaacccgg caacactcca
     6241 cgatatttat gtgcgctcgc cgagcggtgc ccaagtcccc ttgagcgcgg tggcgcgcta
     6301 tgaacccacc tcgacattac tcgcggtaaa ccatcaggga cagtttccag cggtcacgct
     6361 gtcgttcaat atggtgacgg gaatctcgct gggtcaggcg gtggcggcga ttgaacgcga
     6421 gcttcagcag atggctctac ccgccggcat tcggggcagc tttcaggggg cggccagagc
     6481 tttccagtct tcgtcggaca accaaatatg gttgatcctg ggagcgctcc tcacggttta
     6541 tatcgtgctg gggattctgt acgagagcta catacatcca atcacaatcc tctccacgtt
     6601 gccgtccgcc ggtgtggggg cgctcctcgc gctgctcctg ttcaaaacgg aactgagcgt
     6661 gattgcgatg gtcgccatca ttctgctcat cggcatcgta aaaaagaacg cgattctgat
     6721 gattgatttc gcattggagg cggagcggaa agaagggaaa ggaccagcgg aagcgattta
     6781 tgaagcctgc ctgctgcgat tccgtcccat catgatgacg acgatggcgg cgcttttcgg
     6841 cgccctgccg ctggcgcttg gcaccggcgt cggctccgag ctacgacgtc cgctcggtat
     6901 agccattgtc ggcggcctct tggtaagtca gctgctgacg ctgtatacga ccccggtggt
     6961 gtatctctat ttggaccgca tgcgcttgcg tttcctcgga aggatgcacc gatcaatcga
     7021 acccaggcca cttccgcaat agtctcccgg ccgaccgggc gccgcctgtt ctcatcggat
     7081 tcattccact attgacagtc gtcctcactc tccgtaggct gcccacagcc tgaacgcatg
     7141 atgtcccggc tttcgacttt ccaatgggtt tcgactgttt ttctggccgt cacggttctg
     7201 accggctgtc cacgggtctt gtatctggat tatcatccat ccgcctcaat cacgggaagc
     7261 ggaccagtcc gggtggatac cttcacctat gcagggcatc ccacagggct gatgaagcaa
     7321 aaggaagtcg aatccggcga ctatgatccg gagcggctgt ttctgtcaca ggacatcggc
     7381 gaatttttcg cggacgcgct ccggaaggaa ttgacgtcgg gaggctatga agccaggccc
     7441 gactcgatcc gagccgtctc gggaaccatc gaacagttct tccttgatta tgtcggcggt
     7501 caggaacagc ggttccaaat acagacgctg ttccaagtgt cgcgcgaggg cggtccggcc
     7561 ttcacgacat cgtgtcgatc ggagcggcaa caggccaaag actggatgaa aagcggttca
     7621 ctcatcgaac acggcgtccg cgactgtatt gaagcgttcg tcaaagaggc gcaagctgca
     7681 ggagcactgt agtgttagtg tcacgaggac tcaatgccag gatttgaata catcggcgaa
     7741 gcagagcatc agcaagtgcg cgaggtcatg gcatcgggcg tcctgatgcg ctatggattt
     7801 gatggtcctc gaaaggggca atggaaatgc cgtgagctcg aggaaaattt ggcaacgcgc
     7861 ttcggagtcc ggcgtgcgca tgtgtgttcc agtggcactt ccgctctcag tacggcattg
     7921 gccgtgtgcg gagtcggtgc aggcgatgaa gtcgtccttc ctccctttac attcgttgcc
     7981 gatcttgaaa cggttctgct ggccggcgcc gtgcccgtgt tcgcggacat cgacgagacg
     8041 ctctgcctcg atccccgttc ggtcgcggcg gcgatgacgc ccaaaacgaa agccgtgctg
     8101 gtcgtgcaca tgtgtggggc catggccagg atcgacgcga tcatggagct ctgccgaacg
     8161 caccaggtaa cgctcattga agacgccgca caggcggccg gcgcaacttt ccatgggaaa
     8221 gcgttaggaa ccttcggcag ggtcggctgc ttatccttcg actatgtgaa aacgatcacc
     8281 tgcggggaag gcggagcggt gctcaccgac gacgaggaga cctaccgctt ggcacaggcc
     8341 tacaccgacc atgggcatga tcatgctggt gccgatcgag gcgccgatgg acatccgcac
     8401 atcggcgcga acttccggct cagtgaactc cacgcagcgg ttggtgtggc gcaactggcc
     8461 cgactcgacg atatcctgga ggtccaacgt cgtcacaaag ccatgctgaa ggatgggtta
     8521 gcgggcattc ggggtctcca gttccgcgaa ctacccgatc cggcgggtga ttcagccacc
     8581 tttctgagtc tgctgttgcc atctgagcac gatgcccgac aggcggccaa agctctgcca
     8641 gaggctggcg tcgacggctg cttctactgg tacgacaaca attggcacta ccaccggaaa
     8701 tgggaccata ttaaaacatt cacatcaccg ggccgtctgg ccatcgaccg cttcgggtac
     8761 ggcagcgatc tcacgaaaat caccctgccg caatccgacg cagtgatcgg ccggacgatc
     8821 tccatgctga ttaaactgcg gtggaccgag gagcacgtgc gtgatagact cgcccggatg
     8881 aaatccgtgt tggcctgaac tctgccccgt tcgtccaaat tgagccgccc atgaacgtcg
     8941 ccatcattgt cccggcccgt atcgggtcca cacggtttcc gaagaaactc cttcaccgga
     9001 tcaaggacaa accggtgatc gtctggaccg ccgaccgcat caagaaagag gcgccagacc
     9061 tcccgcttta ctttgcggtg gatcacgaat ctttggcgga cgtgctccgc cgggacggtt
     9121 atgaggcgat catgacggat ccggcccata cctgcggcac cgaccggatt gccgaggcca
     9181 atcgccggct caaggccagg tatgtgatca acgtacaggc cgacgaaccg ctcgtgaccg
     9241 gtgggcagat tcgtcagctg gtcgacctta ttcaagctga tacagagatg gcgaccttgg
     9301 gaacgcccgt gcggtacgat aaggattatc gcaatccgaa tcacgtgaag ctggtctgtg
     9361 atgagaaggg ctatgcggta tatttttcgc gtgcaccaat cccgtatcta cgcgacacac
     9421 acggcgcatt cgacgccgag ctggcggcca gcattccggt cctcattcat ctcggcctct
     9481 atgcctacac ggcatcattt ttggagctat tcggcacgct tgcgcccggc aaactggaac
     9541 aggtcgagaa actcgagatg ttgcgggcaa tggaacgcgg gcaccgtatc aaggtcgggg
     9601 tcaccaagga agcgttggtc gagatcgata cgcccgagca ggccgtggag tttgaacagg
     9661 tggtgaccga gcgatttccc gctcattagc ccaacgctcg ctagttcgac aaccagagaa
     9721 gggacgatca tgtaccgtaa tttgcgcctg gtcccccagc tcatctacgg acgaggcagc
     9781 attaatcagc tcggcgacat tctgaccaaa caacgcaccg acgccggatc gcctgtcgtc
     9841 ttcctgatcg atgcggtgca cgaaaaacga gcgttcattc agaaactgcc gctgcgcgcc
     9901 aacgacctgg tcatcctcgt agacgtcggc acggagccga aaactcagta cgtggatgag
     9961 ctcaccgaac ggatcaaggc gttttcaaac cggcagcccg atgcgattat cgggatcgga
    10021 ggcggcagtg ccatggatct ggccaagtcc gtctccatca tgctgaccaa tccgggttcg
    10081 gctgcggact atcaaggttg ggacctggtg aagtatccgg cgatctatca tctcgggatt
    10141 ccaaccctgg ccgggagcgg tgcggaggtg tcccgcacgg cggtcctgac cggacccgtc
    10201 aagaagctcg gcattaattc tgattttacc cctttcgatc aaatcgtcat ggaccccgat
    10261 ttgatcgcga ccgtcccgcc aaaccagcgg tttttcaccg caatggattg ttacattcac
    10321 tcgtgcgaat cgctgcaggg cacattcatc aacgaattta gccgggcctt tgccgaaagt
    10381 ggccgggaca tctgccggga tgtttttctc ggccgctgtg aggacgtcgt cgccgacgaa
    10441 cgcttgatgg tggcctccta cttcggaggc ctcagcatcg cctactcaca ggtcggcatc
    10501 tgccatgcgc tgtcctacgg actgtcctat gtcctgggcg tgcgtcatgg cgtaggcaat
    10561 tgcatcgtct tcaaccagct tgaagaatat tacgggcagg atgtgcgaga gtttcagcag
    10621 atgatccgtc agaaccggat cgatttgccg tccggggtga ccaatggaat cgacgaagcc
    10681 agcatgaata ggatgatcga tacggcgctg ggtctcgaac cgttgtggaa gaatgccctc
    10741 ggagatgatt ggaagcaacg gatcagccgg gaaaagattc gctcactata tgtgaggatg
    10801 taagagatcg agccggccgc atcgtcgcgg ccacctccgc cttccttaag gcggccccac
    10861 ctgtgaacat tcttatcgtc aagaccagcg ccatcggaga cgtcatccat acgctgccct
    10921 ctctttggtc ccttcgcgcg cattttccgg acgcacagat tacctggctg gtggaggaat
    10981 cggcggccga tctcttgtac ggacatcccg ccttgaaccg ggtgctggtc gcacgccgaa
    11041 aaacctggct gaatgacgcg cgtgccggcc gcccgtcccg ggctttccgt ggtgtcttac
    11101 agttcatccg ccagttgcgc gatacccgct acgatctcat tatcgatttt caaggcctca
    11161 tcaagagcgc catctgggtg gcattggcca aaggcggtcg caaggtgggg ttcggccgtg
    11221 gaatggagca tgccgaacac agttatctgg tgctgaacga acgcctcccc gcggtggaca
    11281 tgaatcacca cgccattgac cggagccttc tcctcttgaa aggcatcggt gttcctgcgg
    11341 ctgaccttcg ctacacaatc cctgtgtcgg ctgcacagga atccgaagcc ggagctttgt
    11401 tactcacagt cggcgtgcgt gagggcgatc ggctcgtcgc catcaatccc atgggccgct
    11461 ggccgacgaa gttatgggag cctgcttcgt tcgccgcact ggccgatcgt ctccagcgtg
    11521 aaggtcttag catcgtgttc acgggaggtc cccacgatca cacggccctg gatgagatat
    11581 gccgattgat gacgagccgg tgtcggcggc tcgacggcaa gacgagtctg aatacgctcg
    11641 ccgccatcta tcggcgagca gaagtcctcg tgacaacgga cagcggtccc atgcatttgg
    11701 ctgccgcggt tggcacgtcc gttgtcgcgc tcttcggacc aactgctccc tggcgaacag
    11761 gcccctatgg agcgaaccat gtggtgctgc gcgccgatgt gagctgcagc ccctgcttca
    11821 agaaacagtg ctcgaccacg gtctatgaag agcgggcttg catgaagcgc ttaaccgtgg
    11881 aggacgtcac gcgcgcagtg ctggaaaaaa tttcagcccc gccgctcgcc gcctcgaagt
    11941 gacgccgcat catccgcata tcaacccgca tcagtggatc atcctcacgc tgtaccgtgc
    12001 gctctttacc gctggggtca tcctgttcgt gatctgcttc ccgctctgga tattcaattc
    12061 gaagcgccag cagacactgt tcaaacgatt aggatggcaa cagtacccca gactccaggc
    12121 gtctcgccgg agccccgtat gggtccatgc cgtctcaatc ggggagctac tgtcggctgg
    12181 aagcctgatc gaaagccttc ggcgggaaac cggaggggag cgcccgttgt acctctctgt
    12241 ttcgacactc tcggcattcg ccctcgcaac cgagcgattc ggcagatcct gcgacggatg
    12301 cttttatttt ccctacgatg tgcgatttgc cgtgaagcgg tgcctcgaga agatcaatcc
    12361 ggctctggtc gtgttcattg aaaccgatat ctggcctgga ttcctcgccg atgttcgtcg
    12421 caatgctgtg ccctgcttgc tcgtcaatgg acgattgtcc cccgaatcgt tccgcaccta
    12481 tcgcaccgtc tcagccctgt ttaagccggc gttcaatacc tttcgctcga tttatccgca
    12541 gtcgcagggc gaggcagatc ggtttctcgc gctcgggatt gagccgggcc ggcttcggac
    12601 cccgggcaat ctcaaattcg acgccgcccc gccgatccct gacgatgccc tgctggccgc
    12661 gctccgaaag gagttccata tccttcctga ttcacccatc ctcatcgcgg gaagcacgca
    12721 ttcgggcgag gaggagatcc ttcattcctg ttttcttcgg ctcagacgca aattcccggc
    12781 tctcaggctc atcctggtgc ctcgaaatcc cggacgagga tctgaaatcg tcgagttgtt
    12841 tcagggcgac ggcgtccacg ccgcactctt ctctcgattg gaccgctcaa caccggtggt
    12901 cgtcgtagac cgcctgggat atttgagccg gctttacgcc ctcgccgacc tcgccgtcat
    12961 cggaggaagc tttgtgccga gggggggcca aaatcccatc gaaccagcgg cctgtggcaa
    13021 accggttctt ttcgggcctg acatgcatga cttccccgat gtgtcgagat ggctcctcga
    13081 ggcgggaggc gcgatacgcg ccaacaacga ggaggagctg tacgccgcct gtgaccgctt
    13141 gttaacggat ccggaaaaag ccgctgagat ggggcgacat gggcgccgcg tggtcacggc
    13201 gcatcagggc acgaccgcga ggatcgtgca agatattgtc agcatactgt ccgcgaagtg
    13261 aaaagatatg gcagatgcta acgcagcctc agcgcacgcc cgtcgagctt cgtgtcagca
    13321 ataaattttt cgatgttctt ttcgagcact tcattgacca gttgctgtgc gtcttccggt
    13381 tcaaaccaga acacctcgtc gctcccgctg ctcatgacac gttcctgaat gctgctctcg
    13441 tcgccatggt tggccacccg aaccatcatc gtgttcttcg cttcgagttg ggtatggccg
    13501 accttgctga cagcttgcac ggagagatct tggatggtgc cggaaatcgt ggcgtccggt
    13561 gccgttccgt tggaaccatc caagctggcc tgccacccac gtttgctcag ctgctgcaca
    13621 agcgccttgg cgaccgcctc gccgacggtc ccgttcggca cgtcaaagta actcgtattc
    13681 ccccagagat gcgaccggct ccccagatgc gtacggtcca gtcggacgtc gtcaaacggt
    13741 ttgaccacca cccgcggtcc catgctcccg gagttcgtcg tagcctggtt tggagatgtg
    13801 gatgggatcg cgacgtcgat ccgctcgcct ttggccgcac atcccgccag cacacagaga
    13861 caaacggccg caactggtcc tactcctcgc atcttcgttc ctcctcttgt tggtaatcac
    13921 tccccaacga attccggcag cggcgtggca gtggactact gcacgacaaa atgggcccgc
    13981 cggttttgct gataacagct ttcgtctcgc tccttgcaga acggccggat ttcgccgtag
    14041 ctggtggttt tcattcgcga ggtaggcact ccaaggtcct ccagatagcg cttgacgctg
    14101 cgagcccgct tgtctcccaa caccaggttg tacgcttgcg tgccacgctc gtcgcagtgt
    14161 ccttcaatga cgacggcctt tcccggccgg ttcttgatcc aatcggcatt ggcgccaaga
    14221 atcgtctggg catctctcct gatcgcatac tggtcgtaat cgaaaaagat gtcggacagg
    14281 tttgatgcgt ccaggggaga tgaggcggca gatgaaggcg caggagcagg ggccggctcc
    14341 cccggtgcac ttggaggtga ggtttccgag ggagcagagg ccatgtctcc cggagtgcgc
    14401 attgcctcaa aggaactctt gtcgtccgga atggttgcaa agccctcggg ctggatggtc
    14461 tccaccggtg ctgctgtact ttttctcgtc gaagaggcat caccggacga tcccgcgacg
    14521 cgcttcccgc aacctgaaaa cgtcgtcaga atgatcacac aggatacagc cgctagtatc
    14581 gtatgccgaa tttcccgttc tggcattccc cacctctctc tatagatgaa tggctgcgat
    14641 ccaccgttcg tgcactgcat ggtcgaaggg ttggtcaaaa acaggagatt tatagccggt
    14701 cgctgcgttt ctcgcaaggg gagaaggcgg aaagtgagct gtgatcgggg aactcgtcct
    14761 ccgagcgcac atgaggcatg cgaacggata ccgctatttc actttggaaa aatccgcagg
    14821 aatgcggact tctttgccgt cctgcagatt gatcacaaac acagaggcgg cgttggggtc
    14881 gaagctttca taggcgaaca ccgccgtgaa tcgagaccgg taggcagggc cgcttccttc
    14941 atttttgcgg cctcgttccg ctttccccac atcgatggct ttcactttct tgctgccctg
    15001 ctgcagctcg atgagagcgc cttccgcgaa gtactcgtca tccccgcaca gctgcacttc
    15061 cacttccatg ttcggcatat cgacgacctt cctaatgaat tcgtcgggca tgcgaatatc
    15121 agtttttcgc tttttggact cggaggcttc ctgtcggcca aaggcctcta accggtagcg
    15181 cttggttctg aggatcgcac tcgccccaca ggcatctttt tcgggatcgg ctcccacgcg
    15241 tgtgaccatg gatgcgtgct gaagaacctt tctcacttct tcaggactgt tggctttttc
    15301 cattggtgtg cggccggctt cgagggcctt ttgcgcctcc tctgacgaca gcttcacgtc
    15361 gatggcgagt gccggagtga accccaccca caagagcgcg gccagcagac ctcgcgcccc
    15421 gttcccttga gccaccatga gagcctccat aaaaacgaat gaaggcggca ttttacgaaa
    15481 agcctctccc ctttgcaatc agccggagaa cctggcccag ttggagtagg gcttcgtgtc
    15541 atacaccatg tcgacgtcca cgggaactgt catgccgctc tcctgtaaca tccggacgac
    15601 gagacgaaga ggtaaaccga ccacggttgt aaagtcacct tccagcgatt cgatcaagtc
    15661 ggcgcccggg ccctgaatcg agtaggcccc cgcctttcca aggctatccc ctgttgtgag
    15721 gtagtgttca tgggcttcgg catcaaacgg cttcattcga atgcaggcgg tcgagaggtc
    15781 cgtggcctca aggtgtcgcc cgcgacaggc gattgtgacg gccgtcttga cgcgatgctc
    15841 gcgccccgcc aggtgccgca agattttccg agcctcgaca agatcgcccg gctttcccag
    15901 cacctctcca tctacttcga tcagcgtatc gctggccaag accagggcgt ctgggtcgcg
    15961 ttgtgccacc gaatcggcct tgcattgtgc gaaggcccgg acgagctcgt cggcggaccg
    16021 gtccgacaca aggcgttctt cgtagaccgg atcgcggatg tcgaatggaa tgccgagaag
    16081 ggcgagcagg tcgcggcggc gcggcgaggt cgaggccaac actaaccgcc acatcgagat
    16141 gtcaaaggag ctgcgctttc caaaagaccg gctgacgtct cttcctgatc gtacgcatga
    16201 acgaaccgtt gatagtcggc gagaaccgct tcgagctccg caacggaaga gacgaggaac
    16261 atcctggcgc gcagcgccgc ggcgcgcgga aatcccttgc agtaccagcc gagatgtttt
    16321 cgcatccgcc tgaattgccg actgccccat tgtctttcaa agactcgtgc gtgctccaac
    16381 agcacgcgga accgcgctgt aaggtcgggt gtccacgggg ccaccgccaa tggtcgatcc
    16441 gtctggacac aacgccgagc ttgttccttg tctcgaaaga accagggagc ccctagtgcc
    16501 gcccgtccga cgagtacgcc gtccacccct gtttcggaga cacggcgcac gacgtcgctc
    16561 aagttctgga catccccatt cccgagcaac aaggttccgc tgccgcgagc cacctgaaca
    16621 gcgcgcgcga ttgccgacca atcggcctcc ccccgataca tttgctggag tgttcttcca
    16681 tgcaatgaaa tgaccgcggg agactctgag agcagatgtt cgatccatcg ctcgacgatc
    16741 acttcgtcat acccaaggcg ggtttttacc gagagaggaa cgggcaaacg agcgcgagta
    16801 gccgcaacgt cgcgctgccc gttcaaccgg ttgaccatgt cgactctgac cggtttgaga
    16861 ccggctccct caagcgtctg accggcagtc caatcggcga tgccttgtct cgcagcgcgt
    16921 atgatcgcat gcgcgagatc aggcgtccgg atcaagcctg ccccggaccc agaggaggcg
    16981 acgttccggg agggacaacc catgttgatg tccagaccgt cgaaacccaa ctcgcagacg
    17041 acatgcgccg cttgataaaa caattccgga tctttgccgt acaactgcgc cacaatcgga
    17101 cgctcgctct cgtcatagtg aaggctatcc agcagaaatt ctggtccgcg gcacacgtca
    17161 tggacatgcg tgaactcagt aaagatgaca tccggtttgc cctggctcgc aatcgtatgc
    17221 cgaaaggcgg aatccgtcac tccgtccatg ggtgaaagtc cgatgatcgg cattggcagc
    17281 gtgttccaga atgtcatcgc tgcgactccg cgacggcttc aagatcagcg tgagcgttgg
    17341 cgtcgatgta ggccgagcgc aaggcatccg cttcttgtct ggtcaaggac agcaatgcag
    17401 gtgcccgttc cgagacgaac tccaccagga gatcgatctt cagcaggaag aaatcatacg
    17461 gctgatcgac cgggcgaggt gtatgaaacc accacgtcac aaactggctt aagggaaggc
    17521 cggtttcgtt gaggcggcgg gacgtttctg gaaacatgtc ggtgatgtct cctccccaat
    17581 ggaggatctc atgcggcagg tactcatgaa tgtagcgcga aagccccacg gtatctacgg
    17641 cgacttcctc ccaatttgga ccggtcggcg gctgtcgacc agagacaatc tgtgcgtatt
    17701 gcgcatactc cgccaccagt tgttcgcggt cgacctcgca cgccacaggt gctgtcccac
    17761 cctcgttcgc tggcccacga gtgtccgggg ccagcaggtg gtttgacaaa cagcggtcga
    17821 ttgcagaact aaaccgcctg tgggcatact gaaattcatt cacaatctgc tccatcggct
    17881 tgttgtcgac ttcaagcgcg atcccgcgga gattgcacag tcgggggtga gccaggacct
    17941 gctcaagcac catccaggac acgtccggaa ccggtgcaac gtgggcatcg atccactcgg
    18001 gatgccctcg atcagaatgt cctcgagaca ccgacgcctc atgcggcgcc aggccggcga
    18061 catggacctc gatcacacgc tccagaggaa attcgtttaa gaaccgctcg acgaatgcct
    18121 cgagattcat ctcgcgacag gcgggggaat accggtacac cgtccagaga tgcccgatgt
    18181 caagaacaaa cccgcaggac gtcaaatcag cgacgagccg gaagtaggtt ggaaccgcca
    18241 gagtcccggc cataaagtag gtgagtggcg gcatctccaa caggaacaag ggtccgaagt
    18301 tctggcctgg cgaccgccgt tcatccattt tcgcttgcac aatttcgata ttgtctgcca
    18361 cgactcttgc gctctcggcc gtgtagagcg gaggaagata ggtgccaaaa gagtagccgg
    18421 ccatctgctt ggtcgcgcat tcatggttga gccagggact ggctagaata tgcaattggg
    18481 agatgacatc gtcgagctca tcacgcaggt gcggcgccct tgcgaagtcc ggttgtgtga
    18541 tccacagccc ttcaccgtgg tacgcaaagg aaacaccggt catccggcgg gtgactgttt
    18601 ctaaagccgc cgccgccgct cgaaaaatct ccaggtatgc cggtcgggtt gaggcacgct
    18661 cgaaccggct catgagttca aagagattgg gggagtagac gtcgacggac agcccaaagc
    18721 cttgctgttg gatctcctcc gcacgacact gaaacacctg attcacagag cgttcaccac
    18781 gcatggtcgc cacttgtgct gagacaataa ttggcctagt ctagtcttgg aaccccttgt
    18841 ttgacaaggg aagtgccgct gaggggcagg aaaccctgtc tgctatactt ggcttacgcc
    18901 gcagggggcc ggcctctcac gaatcgtcaa tgaggcacga atcggagagg gtctatcgat
    18961 gctcggtcag aatgaaccgt ccccacaggc ccatcatccc acaccaatcg tggtcgccca
    19021 tatgatgact ccgggagtcg tacaggtgcc gggagacgtt tctgtcagcg aggcggcact
    19081 gctgctcgaa cgtgagcatg caccgtgcct gctggtgaaa gaaaccgaca cggtctttgg
    19141 actcatgacc cctcacgaca ttgtgaaaaa ggtcgtggcc cagggcctgg aaccgcatga
    19201 cattgaagtg cgcagcatta tgacccgtcc ggtccaattc atcgaatatg atgaactgct
    19261 ggaagaagcc tccaccatga tggtgtcaac aggcgcctca ctcttgatcg tgacgaagga
    19321 gaaccagccg gtcggggtgc tcagcgcaca ggatctgatg ttgacgccgg cgcggcgtct
    19381 catgaacatt cccaccatcg tcacggtcgt cgatgaggcg gtgcctcagc gacaggcgca
    19441 taatgcgacc atcgtgcaac tgagccacgt gagcaccagg atcgaatcgg cttccagctt
    19501 gcatccaaag acgcgtgtcg ttttggcgtt ctccctacca ggacagcaca ccccgttgtc
    19561 cattcacggg aaagtcttgt cgtccgagac gcttccagcg accgcgacac atgaatcagc
    19621 cgaacgaacc gtcgccgttg aaattcaatt cgctgacctc tcagccaccg acctggctcg
    19681 cctcaaggcg tggaccctgc aagcgatccc ttcctctctc gatcggccct gattgcattc
    19741 gacgcaactt ctttgaccaa atgcccatca actgataaga tttttctctc actcattatg
    19801 caccaattgg acgccatttc atgaaaacca caccgagccg ttctcgcacc accctgtcga
    19861 acgacgagat cttgaggcaa atccgttcgc tgctcgaatg tccagaggac gacctttcgg
    19921 ccggattgct caaagaactc atggccggaa tcatcaagct tcacgacgct catctcgagc
    19981 cgctcgatct caaaattgtc aaccgcgcca ttaaagagct tcgtcacgcg ttccgcgtct
    20041 ttcgagacta tcatcatcgg aaaaaagtca gcattttcgg atcagccaga acgaccgcgg
    20101 acgatccaaa ctaccggatc gccttcgact tttgccgccg cgttgtcgaa gccggtttca
    20161 tggtcatcac cggaggagcc gacggcatca tgcgggcggc gcaggaagga gccggacgtg
    20221 aaaacagctt cggcgtcaat atcatgctgc cgtttgaaca gggacctaac aacacgattg
    20281 ctgatgatcc caaactggtg accttcaaat actttttcac cagaaagtta atttttcaga
    20341 aagaggccaa cgccatcgca ctgttccccg ggggcttcgg cacccatgac gaagggtttg
    20401 aaatcctgac gctcgctcag acaggtaaaa gcgagcccca gccgatcatc tgccttcagg
    20461 cggcgggctg cgattattgg gacgactggt acggcttcat caagcggcag atgctggatc
    20521 gtcgcttgat cagtccggaa gacctcggcc tcttcaaaat cacctcctca gttcaagaag
    20581 ccatcgatga gattcgaacg ttctataccc gattccactc gatccgctat gtgggacgtc
    20641 agctcgccat gcgtctgacg ggtccgctat ccgcggagca ggtggcggcc attcatgagc
    20701 agtttgccga tctgctgctt gaaggcacgt ttgaactgcg cggcgcgctg ccagaggaac
    20761 tcgatgagcc ggatctgaaa gatttacccc gtctcgtatt cgcctccaac cgccgcagcg
    20821 ccgcgcgcct ccgacagttg atcaaccacg tgaaccgcct ctaaccgatt tcaaatccca
    20881 agactgcgca tcggccctgg agtccccaag agaggggaaa gactcttcca aatgagcctt
    20941 tagtctctca gaatcccgtg gtatgctcga actccatggg ccaattcgac tcgcgacaac
    21001 attttccgct tgcgcctacc gtcgtcttgt accatgcgga atgtgcggat ggttttggtg
    21061 ctgcctgggc cctctggcgg cgctttgctg acgcgcgatt cgttccggtc aagcacgggt
    21121 ctccaccgcc ggacggacta caggggcagc gggtcgtcat cgcggatttc agttacggcc
    21181 gggagacgct ggaaatgatg gcaggagaaa cagatcgatt actgatcctg gatcatcata
    21241 tcaccgccga aaaggcattg gctggtctac cgtacgccta tttcgacatg aaaaagtcgg
    21301 gggcggttct ggcttgggag tgggcgcacg accatcctgt tccctggctt ctggactata
    21361 tccaggacaa agatttgtgg acgtgggccc taccgatgag ccgcgagatc aacgcagcgg
    21421 tggcctcgca tccgtttgac ttccaactct ggagcagttt caaacaaaag gacttggagc
    21481 atgaaggccg tgccattttg cggtacgaac acgaactggt gaacaagctt tcaggacaag
    21541 cggtgctcgt cgaattccag ggggaaaccg tcccttccgt gcaaagtgcg atcttgacca
    21601 gccagatcgg cgagcgactc tccgccgagg cgccgttttg catcatttgg catgatcgtg
    21661 acggacgacg atactacagc atgcgatccc gcgaagacgg aaccgatgtc ggtgcaatcg
    21721 cggcatcgtt cggcggcggc ggccataccc atgctgcggg cttctccgtt ccgctgggtc
    21781 ctgatggctc gcttccggac aatccggcgc tcccacggtc catcgtcgac cgtcgtcgca
    21841 attccaccta agcggattca tgatcccgct tcaagacgac aatccctccg aacgctctcc
    21901 ggtggtcacc atcggcttca tcgccatttg cgtctttgtc tttttctatc aggccagcct
    21961 gcctccgcaa ccgggagaga tgttcgtctt tcagtacggc gcgatcccag caatattgtt
    22021 cggccaagcg gatctccccc ccgccattgc cgtcggcatt cccgcttatg ccaccgtgat
    22081 cacaagcatg ttcttacatg gtggatggat gcatttgatc gggaacatgc tgtacctgtg
    22141 gatctttggt gataatgtcg aagatgtgat gggacacgtc cgttttgtcg tgttttacct
    22201 cacgtgcggg atcttggccg cgttgagtca cgccctcacg gatccgacct ctacaattcc
    22261 catggtcggt gccagcggcg ccatttccgg cgtcctcggt gcctacctcc tgctctttcc
    22321 tcatgcccac gtgctggtgt ttctgcccgg tatcgggatg acacgagtgg ccgccggtat
    22381 tgtcctgggc atgtggttcg tcatgcagct gctgagcggc ggcatgagta tagggggacc
    22441 gggcggagga gtcgccttct ttgcgcacat tggcgggttt gtcgcgggga tggccttgat
    22501 tggcttcttc aaacgtccgg aagtgcggtt cttctcgccg gccaagaccc gccgatggga
    22561 tctctagaaa agcggctccg ccgctccgaa ggaagtgacc tgctaggcat cgatcagctc
    22621 acgcaccttt tccaccaacc ctcgttgacg atacggccgc ctgagcagaa atcgtcggtt
    22681 gatgcggtga gagatgatgg ctgcatcatc gtatcctgat acgaacaggg ctttcatcgt
    22741 cggatgctgc ttcaacagcc gtctcgccaa ctcacgaccg ctgatttctg gcatgatcag
    22801 cccgctgacg gtgacatgta cggctcctga atattgatgc gcaagcatga tggcttcgac
    22861 gggtgagctg gcttcgagca cgtgataccg atgccgcttg agggtcgaaa ccgcgagttt
    22921 ccgctcgagt tcatgaggct cgacgagcaa aatggtctct tggcctttcg cggcgacggg
    22981 aaattctgcc ggctcgtttg gaaccatccc ggcctcgacg gcgggaagat agatccgtac
    23041 cgtcgtccca tggcctggct gactggcgac ctcgacctgc ccaccgtgct gcttcaccac
    23101 tccatagact gacgtcaatc caagaccgac gttggtctct ttcgtcgaga aaaacggctc
    23161 aaacatccgt gactgcgtat ctcgtgtcat cccgcttccg gtatcgcaaa cctggatccg
    23221 aaccgccgat gcgcatctgt tcacgccggc aagcgctttg acgtctgctc ctccttcgca
    23281 cgaggaaatc gaaatggtca gacgcccccc tcccgtcatc gcatcacggg cattcaaggt
    23341 gagattcaac aacatctgtt caatgccttc gcgatccacc tcgacgtcca ggtggctgcc
    23401 ttcagccgaa atttgacagt cgatccggct cggcaagagg tctcgaatcg tttcactgag
    23461 atcttccatg agcgtccgca tggacaggac gtgacggaat tggcgatgct ggatatcgag
    23521 cgccgctagc tgggctgtca gcgcagctgc ccgttctcct gtccgaaaga tatcatcgac
    23581 cgctccatac aaaggatcct ccggaaggag gtgcgacagg accactcccg tctgctttcc
    23641 gatgaccgag aacagatggc tgaactcgtt ggctaagccg gatgtcatcc ggccaatggc
    23701 ttccatcttt tgcgcctcga ccaactggcg ctccaacccc tgccgctccg acttctcctg
    23761 ctggacaagc gcattggcct tcgacaaatc ttccttgagc cctttgatgc ggatctccaa
    23821 ttggtcgcga gacgcctcca aagcctgttc ggtctttttt aatgcgtcaa tgttgtgcag
    23881 taactgcgtt ttttgctgcc gctctgctcc gatggtttca agcgccatcc cgtagaaaat
    23941 tgccatcacc agtaacaccg gtacgcccaa caattgaccg actgacagca cacctgtctg
    24001 gacactcccc tggtacaaca caaatccgta tcccgcacac agtaacaacg aaaaggcgat
    24061 cacatgagac agccgacgca gagacgctgc gatcagcatg aggatgaagt aggacagata
    24121 cagttctgat ctggcctgtc ccgacaaata catcgtgccc gtcaccaggg ccgtatcgac
    24181 cgcgataagt gtgccgctga accaggccgc ctggagcacg gaggaaggga cggccagtaa
    24241 gcacccgatc aggaccacca atccacccac gatgaactcg cttgccgctc gactgacgac
    24301 gagctccggg ccgaacagca actggtagcc cagcatgatg gcgaccagca actggaggat
    24361 cacatagcga ggccgtgaaa cggctccctg ttcgtctgtt tctggtgatg aaatggagtc
    24421 tggagaaaag gccagcgtgc cgtgcatcgt tcctccgcct gcaatgccgg ccaagacacg
    24481 agcaagtccc gtaccctcgc cctcgtccag aaggaacttg attttgttga gctctaaagc
    24541 gcgctgctga tatttggact acaagacgtc acaaaatgga acggtcatgc ggttccagaa
    24601 agtccaacta aattgggaag agccgttcaa ttgcggctgg cttggagaag gctctgccgc
    24661 aaacgcgagg ccctgtctcg agcggtctgg acatcatggg ccagaaacgt cacgtgcccc
    24721 attttgcgcc ggggacgtat cgcacgtttg ccgtagtcat gcacgtaggc gccgggaagg
    24781 ccgagcaacc gacgacattc gacatccctc ttgaccgtct ctgcgtcctc gccaatgaga
    24841 ttgaccatgg ctgctggatt cgtcagcagg acttcttccg gtggcagacc cgtgacggct
    24901 cgaacctgct gttcgaattg cgagacattg catgcatcca gggtgtaatg cccggagtta
    24961 tgaggtcggg gcgcgacttc attgatgagc acgcgttgat caggcaacag aaacagctca
    25021 agacaaaaca gtccgatccc gtccatacga gcgaccaccg ccctggcgag gtcggctgat
    25081 cgttccacca cattggaagg cacctctgca ggcacgacgg tcatgcggag aatccccgcc
    25141 tcatgcacgt tttcgaccaa gggatagacg atggtctgtc catcgatgcc acgagctacg
    25201 agaacggaaa gctcacgctc gaatggcaca aagctctcga cgatccattc catcgtgctg
    25261 tgacaggacg ccgtcaggga atccctgacg gaaaacacgg acgacggctc atcgattttc
    25321 cattggccct ttccgtcgta gccggcagtc gcggtcttgc atatcgcagg gtagccgagt
    25381 tgttccaaaa cctccgctaa tctctcgggt tcagtcaacg ccgcgaatcg gggaacggga
    25441 aagccctgac ccgcgagaaa cgtcttttgg acgaggcgat tctgtatccg ccgtaacacg
    25501 ctgctggctg gccgtaccgg tgtggtccgc tcaaggcgct cacagagccc ggccggcaca
    25561 ttctcccatt cataggtcac cacagctacc agcccggcaa actccgcgaa ggttttctga
    25621 tcagtgaatg gcgatggaaa ggaatacgcg gcaaggtgat gagccggcgc atcggggtcg
    25681 ggatcccaga cggcaacgcg gtaatcaaga cgggtcgcag cttgcgcgaa catggcgccc
    25741 agttggcctc cgccaagcac cccgagcacg gatccgggct cgaccaatgg accgttcaca
    25801 ggcgacgcgg ttgatgcagc gatggcttga aatctgccac ggaacctttc gcctccggag
    25861 actgcagcac atgcgtcgtt tgggcatgcc gaaagctttt gacgcgaacg gcgacctcgg
    25921 gatagcgacc ggccagaatt tgggcggcga gtaagccggc gttttctgct ccgccaatgg
    25981 ctaccgtcgc cacgggtatg ccgcgaggca tctggacgat tgacagcagc gaatccagcc
    26041 cgcggaggtg ttcggttgga atcgggatgc cgatgacggg caaatgcgtt tttgccgcga
    26101 gcatgccggg caagtgggct gcccctcctg ccccggcaat gatcacttca atacctcgcg
    26161 cgtgcgcctg ctcggcatat gcaaacaaac gatcgggcgt acgatgtgcc gagacgacga
    26221 tgagttcatt ggcaattccc aactcattca agatggccac ggccttttcg agaatgggaa
    26281 aatcagactt gcttccaccc agcacaccga cgatcgggcg ggaaccaccc cttttggttc
    26341 ggggtgaacc gcctcgcttg gatgtttccg gcacgatatc gctcccttag acaagctcct
    26401 gtggggtcca caccttaacg gctcttcaca cacgggtcaa gcaggcagac catgcgttac
    26461 ctccgcgttc tgcaagagaa attgacccat tgaaccattt tcctctatga tgaagcctca
    26521 caattccgcc gcgcccgaag ggagcttctt cggtggcagg tatcggacac agtctccgtg
    26581 aaagagttcg cctgctcgtc caacggccta ccggactgga tgaaattgcg gtggacgatt
    26641 ttgcggcgtc cagtaagctc cgggcttggg aagcggtaca gattccggag cggctcaggt
    26701 tctcttcccg ccttgccatt aaactggtcc tcctgttcct tctgattatc gcgtttgtac
    26761 cctggacgca gaccatcacg gtaagcgggc aattgtccgc ctacacgccg tacgaacgac
    26821 cacaggacat tgaagcgcag atcacgggtc gtattaagaa gtggcacata ttcgaaggtg
    26881 ttcgtgtcaa acagggcgat ttgatcctgg agttggacga ctacgatccc aacttcatgt
    26941 cccctgatct gctccccctg ttagaacaac gcaagaaagc cttgaccgaa acccggcagg
    27001 ccgccttggc acgcgccgag caactggata aacgcatcaa ggaaatgcag aacctggtca
    27061 aagccgcagt gccctccgcc caggcgcgcg tcgtcgaagc ggagaaccag gtgcgcgagg
    27121 cctatcagaa ggtcgaagca gccaagatcg cggtggccac cgctgaattg aacgttgacc
    27181 gccacaagca actggccgaa cagggtctcg tctcccagcg cgagttagag gtgacgatcc
    27241 aggccgccat cgccacgaaa gccgatttga cgggcgccca agctcatctg agggcgaccg
    27301 agcaggcgat gaaagcgttg agtttcggac gggagcagat caatgcggag gtcttgcaac
    27361 gcctgttgga cgccgaggcc gcccgggatg ccgcggtcgg agaagcggcc aaagccacgg
    27421 atcagttggc cgatgtctcc ttacgtatgt cgaatgctga acagcgccgc gtggcaggcc
    27481 gcatcatggc gcctatcgat ggcaccgtgg tgaaaatggc cgagacaggc atcggcgaaa
    27541 ccgtccgtcc cggggacaaa ttggtaaaaa tttcgccggc cagcgccgac aaggccatcg
    27601 agatggtcgc agacggcatc gacgctcccc tgttgaacgt cggacgcaag gtgaagatcc
    27661 ttttttacgg cattcccgcc attcccttac ccgcatggcc ggaattgatg gccggcactt
    27721 acggtggcgt gatcaaagtc atcgaccagg ttgacgacgg aaaaggcaat ttccgctttt
    27781 gggtggtgcc tgacgcggag gaaaaaccct ggccccccca agaacatgtc cgccaaggca
    27841 ccaaggtcat gggatgggtc atcctcaacc gtgtcccgtt atggtatgaa ctatggcggc
    27901 gcttcaatct cttcccgccc gactatcagg aacgtccgcc aagcctcatc gataccctct
    27961 tgccgaaggc aggacgaggt gcgaagtagg cgccactcag cttctcttca ttcgaatcta
    28021 tcgttgcttg tcacgtcctt ctcgatcacg aaatgtgcgg tgcgatcccg gcgaagagca
    28081 tgctgttaat aggaaaggag tgtgtcgtat gcctcatcca tatgtactcg ccattggcct
    28141 tctaatcgcc ctgggcatcc cctggagtta tgcgagcgct gccgatccgt ccaccaaacg
    28201 ggaactcccg ccaatcccgc ttagtgtgga tgaaattcat gcctgggtcg atcgctcgca
    28261 tcctctcttg aagggtgccg gaacggaaaa agtgatggcc cgcggcagga tgctcaaggc
    28321 gttaggtgcc ttcgagccca acgtcgtgaa cgatactgaa cttgagcgat tcatttcgag
    28381 cagtaatccg aatgcaggta cccagacggc cgggttcaac gacacactgg tggaagccag
    28441 acacccacta ggcttccgct acagtgctgg catccgccaa gcgatcggcg atgcgagaat
    28501 tcccgatctc ggcttcaata acgatagaca acaggttctt ctcggtggat ttttcccttt
    28561 gttacgcgga ctcatggtca atcctgagaa tgccgagctg cagcgatcgg aactggccga
    28621 cccccgcgcg gatgtccgga tcgcacaaac cagacaggac ctctttcttg cggcagccac
    28681 gcagttttgg gattgggttg cggctgtgaa gtttcttgag gtccaacgtc gcgcagtcgg
    28741 gattgcagaa gaccggttca aacaggtcga aggccgcgcc aaggccggag ctgttgctcc
    28801 actggatgtc gtggaagcga accaggaagt tcaacgccgg cgggaagtgt caattgccgc
    28861 ccagcgcttc gtcgagcagg agcagttgaa gctctcaatg tttctttggg aaaacaacgc
    28921 ccccgtgaat ccgccattgg agcgggcacc agaattcccc tctcaaatgt tgatcccgac
    28981 tccagaaacg atcaaggctc acaagatcca ggccaaggcc gatcgtccgg agatcaagga
    29041 gatcggcatt gaggccaaga tcaacaacat cgatcttgaa ctcgccaaga acaatctgct
    29101 tcccagcctc gatgccgaag cggcgccggc gcggcagccg gaaaagttcg tgctgggtct
    29161 gggctatcgt tttggattgg aactccggat acccattctc cagcggagaa gccgggggga
    29221 agtgctggaa gcgcagggca aggccgatcg tttcgtcctg acacagaaat tccgcgaaca
    29281 gcaagtggtc atcgatgtgg ataatgccct gtccgccatc gagcgggcca aggaacggat
    29341 cgctgccgcc gtggagtcgc ttcgtttggc gaagacgctt gaggaaggag aacgattccg
    29401 cttcagttta ggcgccacga gcgtgctgtt cgtcaacctg cgtgagcgca actccgtgga
    29461 ctcggaaggg caggtcattc gagccaaggc cgattatcaa aaggcgctgg ccttgtatca
    29521 gtggtcaatc ggggcttggg ccaaagccct gccttcgagc atccctgtca actaccgggc
    29581 gcgggactaa gaccgaagag ttggcttttt cccctgtact ttgctagcgt tagggagcgg
    29641 ttctggatgc ggcttcaagg ccgcttgatg aaaaaaggcg ggtgccagtg cagagtcatg
    29701 ttgacgatca gcccaacctc tttcagtcgg tggtcggcta cgtcggcgtg cttttacggc
    29761 ttgagcggcg cctgctcgcg ctcgtggttt cctactcact ggcgatcggc ttgttcgcgc
    29821 tgatcgtccc tctgaccgtc caggaactga cgaacacttt cgcctttgct atccagcccg
    29881 tgacgatcgt cacgctggcc ggcgtgatga tcgctgccct cctgttcgtg ggggcgttcc
    29941 gctcgttgca atattatgcg gtcgaagtgc tggaacgacg gcttttcgcc cgtgttgcca
    30001 tcggaatggc acagcagatt ccgcaattcc agtttctcgg ctttaaaccc cggtatgcca
    30061 accatttcat ggaaacggtg ttcatgcagc gggcactctc ggtgctgctg gtggacctga
    30121 tcaacgttgt cgtcggcggt gccgtcggaa tgacgattct ggtcttctac catccgtatt
    30181 tcttgcttta taatgcgctg ctcctcgccg gattcagcat cgtgtttctg ttgtcgcacg
    30241 gggggctcaa ggcgaccatc gagatgtcgc acgccaagta cgacaccctc cattggatgc
    30301 aggaaatttc gtataacctg ctgcacttca aagccacgga tagtcaggct atcctgatgc
    30361 agcggaccga cgaactcgtc ggcaagtacg ttcagacaag acagacgcgt ttcgggattc
    30421 tcattcggca gtacctgggg tcggtggggt ggcaggccgt cgcacacggg ggattgattg
    30481 ccactgcggc ttggctgctg tccatcggcc agctcacctt gggccaactt gtggccgccg
    30541 aagtggtcgt cagcggcctc ttggcgagtt ttgacgaagt cgtcaagcgt atggggcaca
    30601 tattctactt catgacgggg ttgagggaac tggatttggt gctgtcgctc ccgaaggacc
    30661 aaggctcgtc cgctttgtcg gtcccgcttc ctgatccgac cgtgcacggc atccggttga
    30721 cgtgcaagga cctcacggtt catcacgcag gcgtcccggc gatcttcgag cagtttaatg
    30781 tcgaagtcac tccaggcgag aagatcggca tctacgcgag cacgacggcc gcgaaaacgg
    30841 ccctggcacg cgtgctagcc ggcctggaga tgccaactgg cggcgtgatc cgctacaatg
    30901 gcgtggacct gcggcatatc gacatgaatg ccatcaacag gtgccgcggc ttcatgatcg
    30961 attcccagtt gacgctgttc gaaggtacaa tcgaagacaa tatcctgctc ggccgttcct
    31021 atattcccta cagtgatgtc cggtgggcat tgcgctttgc cgaactggaa gaggacgtag
    31081 acaccctccc gcaaggcctc aaaacgcaca tccgggcgcc tgggaaaatc ctggccccga
    31141 cacacatgat gcgcatctta ttggctcgtg cgattctagg gcggccgcag attctgattt
    31201 tcgatgggat ccttcacacg ctccagcctg ttctgcgtga gacgttgctc cggcggctct
    31261 gttccaaaga cgagccctgg tcggtcattt tcgtgtccaa tgatccgaac ttgacgccgc
    31321 acgtcgatcg gcggcttatt ttggagtaag gagacacccg tgttgctctt gcggtggcta
    31381 cgctcgtttc gtcccagcgc ttcgcaaata ttgatcagct tgctgattgc aggcctgggc
    31441 tggttgagcg gcggcgcgct cagccgggtg gatcaagacc tgaggatcat gtacacggaa
    31501 tacacgctcg gcgccgccga tctggcacac atctcggcag atgtcatgcg gtaccgcaac
    31561 accatcattc gagcattgga agccggcagt cgaaaagact tcgaacgcat cactgagtca
    31621 cttcccggcc agcgggcccg ggtgcaactt gccgtcgatc gctatgcggc cgcaggcctt
    31681 cgcgtttctc gcagcggaag gagcgagccg gaggacatcg aagccgtccg gaaaagcctg
    31741 gatcagtact tttctgcggc cagcaccacg atcgacttgc tgtccagaga atggaatgcc
    31801 tcctcaccca gcgaacggga tcggttacgc cgcaatgcag aggaacatgc cgcagacaat
    31861 gccggcccta agatgatcca ggtcagtctg gcgctcgacc ggttgctgga cacggtcgcc
    31921 gatgtggcca aagatatgcg tgacgagggc accaaagcga ttcagtcgac gagcgtgatg
    31981 ctcgttgtcg ggagcttctt catcgcggtg ctgaacctct tcctgaccag accccgtact
    32041 ccccgcggac aggaggccgg tcggtcgcca gaaccccttt ctgaaccgtt gcgacccacg
    32101 ttgccccttc gacccgggaa tcaacagcat ccgatccgct cctaggagtg ctcagcatcc
    32161 tcgtggcttg cggaactccg cctgaccgca cgaaagagtt cctgttgctc ttccgtggtg
    32221 aggggcttcc acctccccga cgataggccg gccaacgtca cgtgcatgat cctgactcga
    32281 tgtagagcca ccacccggta ccccagcgcc tgacacattc gccgtatttg cctatttcgc
    32341 ccttcagtta atatgatcgt gaatcgtttc ggactcaggc gctggacgcg acaaggctgg
    32401 gtcttcttgc caagaatgac gacacccttc gccaggcgat cgagaaagga ctgatcgtag
    32461 ggtcggtcga gccccacctc gtactcgcgc tcgtgaccga actcggtgcg caaaatctcg
    32521 ttgacgatgt cgccgtcgtt ggtcagcaga atcaagccag aggaatcctt gtccagccgg
    32581 ccgatgggga aaatccgttc cggatgcccg atctcactga tgatatttct gggtacgtga
    32641 ctttcgctgg tcgtggtgac acccaccggc ttgtgatact tgatatagac tcgcgtctgc
    32701 ccccacggga tcacgcgccc atcgcaggcg atcacatctg cggtgctgac ctggtcgccc
    32761 agcttcgcga cagtggcatt gaccgtcacc cgtcccgcct cgatcaatcg atcggcttcg
    32821 cgtcgcgagc agatgccgtg ctcggtgaaa aatttgttga tacggatctt atccacaccc
    32881 aacggaggac tattgcgcag gatgctcaaa ctgaccgttc agcaaggccg ccacgagcgg
    32941 ctgactcgaa gcggacgttg ttcggtccgg taaagcttgg agggatgtga gaacgaaggt
    33001 ggcggtattt gcagtgccat ttttcagtgg acccggcggc ccgctttgat gcggccgagg
    33061 atgcgataaa tgagcctcgc gacgcagaac tgcggggcga cgaacccttc aatcggagct
    33121 atttcgctga tgtccatacc cagaatcccc ggcccattag cgagtgccgt aatgagattc
    33181 aacgtgtcat accagcccaa cccaccgggc tccggcgtgc cgagcgccgg caccaacgac
    33241 gcatcaaggc cgtcacaatc aaacgtcagg tacaccggcc cctggcaggc cttcacgacc
    33301 tgaggaatcc agcgactcgc tttcccttcg tacggaccgg acggatccaa caattcggca
    33361 gcaaaaaaag tggcaattcg atccgtccgt ctgatgcgct cgatttcttc gggacagatc
    33421 gatcggatgc caacctgcac gagcggcaat ccatcatcca cgacgcgcgc catgacgctg
    33481 gcatggctgt acaaattgtc ctgataggcg tgccgtagat cgccgtgcgc gtcgatttgc
    33541 acgacgctca tgttcggata ccgcctggca tgcgcacgaa tcgcccccag cgctcccgtg
    33601 tgttcgccgg tgagcgtcac cagaaaacga ccgttgccga tgtgggactg cacaaactgc
    33661 tcgatcgcgt ccaccgcctc gcggtcgatt ttacctttca gttcgagcgg ggccaccgtg
    33721 gcgattccgc cccactccct aaaaggctcg cagcgaagcg tttcatcgta acactccacc
    33781 tggtgagaag cttcaatgat ggccgaagga ccttcatcag agcccagaat gtagctggac
    33841 gtatgctcgt agggtgcagg aaggacgtac acaccggcct ggtccggatg acaccagggc
    33901 tcttcaagac caaggaaatt atgttgggag ccttcccatc ctgcgggaag cgtcatggat
    33961 atgtactcgc aggccgcgag aaaaaccact gcgccggcgg accttggcag tcccgattaa
    34021 cgacgagccc gcttcggcag attctccaga ggaatgaata cagccaatgc cacgacagtt
    34081 gtccatcggc cgggttttcc aatggccgac tgcgtaatgt tcagagtccg cacgatcttt
    34141 ccacccatct taaacacttg ttctcgttct ttccaagcga tgttgggatc gaattccacc
    34201 cccaacgtcg tggccaacat ctgcgcagcg aggtcctctg tgtattctcc cgtttcctcg
    34261 tcggtttctc cgtgagcgtg atgctcggac aaatagccgt acgtgcggcg gtccgtcggg
    34321 atcgccaagc cgatcgacgc tgaaatgaga cggttacgct cgttggtttc tgaacgcgcc
    34381 atgacgcagt gcgtgatttc gccaggattc aggagttttt cgccacgctt ccgagggata
    34441 attttgcaat tgggcggcag aatggacgag acgctgacga ggttgcagt
//
LOCUS       DASSCQ010000123         5694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_467063, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000123 DASSCQ010000000
VERSION     DASSCQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467063"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFH05_08440"
     CDS             <1..276
                     /locus_tag="VFH05_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6678283.1"
                     /translation="HARLKTRQLLTSQAHGQSIRRIVVEGTPFVEIGRFARAEGIDLI
                     VLGSYGGRSDSMEKIFFGATADKVVRTAGCAVLTVPLRQRTRKDIVL"
     gene            273..1220
                     /locus_tag="VFH05_08445"
     CDS             273..1220
                     /locus_tag="VFH05_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6678284.1"
                     /translation="MKTTSITRLLLATDFSDEARRAEEYASALAASWQIPLSVMTVLE
                     FPPGMNPDYAVNKQYLTDRMHDASTRLAEFKERIRRRGIVATTHIVNGIPSERVIAAA
                     QSEKADLLVVGTRGKSGLAHVLLGSTAERVIRSAPCPVLAVPTLKDDLSTEESISLKR
                     ILVPTDFSDCSLDALEYAAVVASQSKASVELLHVLEPVYYGLDFTFEHAGERERKRKQ
                     IAQVLEDVSADLSKAGILVKTGIRGGIPPDTILEYVQTSASDLIIMGTHGRRGLSHLM
                     TGSVTEAVLRRGRCPVLAVRKLPFRDGVRRAIVSVGQSS"
     gene            complement(1268..4015)
                     /gene="fdhF"
                     /locus_tag="VFH05_08450"
     CDS             complement(1268..4015)
                     /gene="fdhF"
                     /locus_tag="VFH05_08450"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET6678285.1"
                     /translation="MNVEATDMQITINDRTVQAESCDTIYVAAVRGGIHIPSLCASAH
                     LSPYGSCRLCLGEIVGLAGLHATCSMPVRDGMVVRTESETLTRHRRNLLELYLSESLD
                     PASTQELQKLAQAYHVTSVRYPAGAKRAPIRDESNPFFIFDNARCISCARCVRACDEI
                     QPAFALSMVGSGFGTRPAWGRGGISGSGDIASSTCVSCGACVKECPTGALTEKTAYRQ
                     GPPATSVRTTCAYCGVGCAFDAGVRDGNVVQMVPADDGPSNLGHACLKGRFGWTYVTA
                     PDRLRTPLLRSGGTWKPIPWAEALDRIAGELTRITAQYGPDALATISSSRGTNEENYL
                     FGKFMRCVIGTNHIDNCARVCHSATVSGMMETLGASAATNSIKDFDLAKLILVVGANP
                     TESHPVLGARIIQAHRRGIPLIVIDPRRTELARLADLHLQLEPGTNVALLNGMGHVIV
                     EEGLIDRTFTSGRTQGLEEWVNAVAEYPPEKTERITGVPARLIREAGRRFASSGASLS
                     VHGLGMTEHRWGSHGVMSLVNLALATGNIGKPGTGINPLRGQNNVQGASDVGCLPTYF
                     AGYQPLDDPGLALLHQRITGRPLPAKRGMKTPDMWDAAIAGRLKGLWIIGYDVAQTDP
                     NLKKVHQALRNVEFLVVQDLFMSETAKLATLVLPAASFLEKDGTFTNLERRIQRIRKA
                     VDPPDGILPEWQVVCEVSARMGYPMTYTHPSQIMDEIAKLTPMLAGVSYDRLDDHAGL
                     QWPVLSPTHAGTELMHEKAFPKGKAHFVAVDYLPPGETASEQYPFILTTGRILQHYNC
                     GAQTRRTALLNIVDADVLEMHPIDMEQLDLDDGATVRLLSARGQALLPAVKSERVLPG
                     HLFTSFHFPDSTVNELLSSSADESSKCPEYKVSAVRVEPVDRDAADHAELRRTRRRLI
                     L"
     gene            complement(4012..5535)
                     /locus_tag="VFH05_08455"
     CDS             complement(4012..5535)
                     /locus_tag="VFH05_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET6678286.1"
                     /translation="MATQLYLSNDTSARAAGADQLAEAWSLDPDIQITRTSSRGAFFL
                     EPLVERDGPNGRLLWPLVKPDDLARIKAGLGGIEISEVPFLTRQQRAVFAQFGMAEPL
                     SLAYYRATNGWRGIERAAGLSPDGIIAEMKVSGLRGRGGAAFPVWQKWDIARRTHAEC
                     KYVVANADEGDAGTYCDRMIMEGEPYRLIEGMLICAKAIGADRGYIYCRWEYPAAAAT
                     IQAAIDACERGGLLTVDDKPFQLEVFRGAGSYVCGEETALLSSLEGGRGMVKVRPPYP
                     AVAGLYGKPTIVSNVLTFATVTEIFSRGAAWHASLGTARSKGTVALQLSGRIKQPGLV
                     EIPFGASLGEVIEQFGGGMADGAQFKAVQVGGPLGSLFPSSQLDIKLCFDEFHEAGAV
                     LGHGGIVVFDHETDMVDLARHYMTFTADESCGKCAPCRIGSVRGRELLERIQNGQGTA
                     ADLALIADLGDTMKATSLCAHGARGPYPVLTAIEHFGDEFHQRLQARSGHIEGGSRR"
BASE COUNT         1213 a         1678 c         1617 g         1186 t
ORIGIN      
        1 cacgctcggc tgaaaacgag gcagcttctc acgtcacaag cacatggtca gtcgatcagg
       61 cgaatcgtgg tggaagggac cccctttgtt gagatcggaa ggtttgcccg tgcagagggg
      121 atcgacttga tcgttctcgg cagttacggc gggcggtcag atagcatgga aaaaatattt
      181 ttcggagcga ccgccgacaa ggtcgtccgg acggcagggt gcgccgtgct cacggttccg
      241 cttcgccaaa gaacaaggaa agacatcgtg ttgtgaaaac gacatccatt acgcgtctgc
      301 tcttagccac tgatttttcc gatgaggcac gccgagcaga ggagtatgcc tctgccctgg
      361 ctgcttcctg gcaaattccc ctcagcgtga tgacggtgct tgagttcccg ccgggcatga
      421 acccagacta tgctgtcaat aaacagtacc tgaccgatcg catgcatgat gcctccacta
      481 gactggcgga attcaaggag aggatacgca ggcgcgggat cgtggcgacc acgcatattg
      541 tgaacggcat tccaagtgaa cgagtgatcg ccgcggctca atccgagaaa gccgatctgc
      601 tcgtcgtagg cacaagaggg aagtcgggat tggctcatgt attgttggga agcaccgcag
      661 aacgggtgat acgctccgca ccgtgccctg tgctggcggt tcctacgctg aaggacgacc
      721 tgtcaacgga agagagcatc agcctcaagc gcattcttgt cccgactgac ttctctgatt
      781 gctcattgga tgcattggaa tatgctgccg tcgttgcaag ccagtcaaaa gcgtctgtcg
      841 aattactgca tgtcttagaa cccgtctatt acggactcga tttcaccttc gagcatgcag
      901 gtgaacgtga aaggaagcgg aagcaaatag cccaggtgtt agaggatgtt tcggccgact
      961 tatcgaaggc gggaattctc gttaagacag gcatccgtgg aggaataccg cccgatacca
     1021 tcctcgaata cgtgcagacc tcagccagcg atctgattat catgggcacc catggccgcc
     1081 gaggactgtc gcatctcatg acgggaagcg taactgaagc ggtgttacga cgaggacgct
     1141 gtcctgtgtt ggctgttcgc aaactgccct tccgcgacgg ggtgcgccgc gcaatcgtgt
     1201 cggtcgggca atccagttaa gccgcttgag gtccgactgc cgcgctgcac cctgcacctc
     1261 cctaccttca caagatcaat cggcgccgag tccgtcgcag ctcagcatgg tctgccgcat
     1321 cgcgatcaac cggctccacc cgaacagcgg acaccttgta ctcgggacac ttcgagcttt
     1381 cgtctgcgct ggacgagagc aactcgttga cggttgagtc gggaaagtga aaactcgtga
     1441 agagatgacc aggcagcacc cgctcgcttt tcaccgccgg aaggagcgct tggccacggg
     1501 cgctcagcaa tcgcacggtt gcgccatcat ccagatctaa ttgttccata tcgatcgggt
     1561 gcatttccaa cacatctgcg tcgacgatgt ttagaagggc cgttcggcgg gtttgggctc
     1621 cgcagttgta atgctgaaga atgcgaccgg tcgtcaggat gaaaggatat tgttcgcttg
     1681 ccgtttcacc cggtggcaga tagtcgactg ccacaaagtg agctttgccc ttgggaaatg
     1741 ccttctcatg catcagttcc gttccggcat gagtcggcga tagcaccggc cactgcagtc
     1801 cggcatgatc gtccagccgg tcgtaagaca cgcctgccaa catcggggta agcttggcga
     1861 tctcgtccat gatctgagat ggatgagtat aggtcatcgg atagcccatc cgcgccgaca
     1921 cctcgcagac gacctgccac tctggaagaa ttccgtcagg aggatcgacg gctttgcgaa
     1981 tccgctgtat gcgccgttcg agattggtaa acgtgccgtc tttttccaaa aaagaggcgg
     2041 cgggaaggac gagcgtcgct aatttggccg tctcgctcat aaacagatct tggacgacga
     2101 gaaactcgac gttgcggaga gcctgatgaa ctttttttaa attcggatcg gtttgcgcaa
     2161 cgtcatagcc gatgatccaa agacccttga gtcgcccggc gatcgccgcg tcccacatat
     2221 ccggcgtttt cattccgcgc ttggcgggca atggacggcc tgtgatccgc tgatgcagca
     2281 acgccagtcc cggatcatcc aacggctggt atccggcgaa gtaggtaggg aggcacccga
     2341 catccgaggc gccttgcaca ttattttgac cgcggagcgg attgatgccg gttccaggct
     2401 tcccaatatt tcccgtcgcc aatgcaagat tcaccagcga catgacgcca tggctgcccc
     2461 aacgatgctc ggtcatgccc agtccatgca ccgagagcga cgccccgctt gaggcaaatc
     2521 ggcgacctgc ttcgcgaata aggcgagcag gcaccccggt gattcgttcg gtcttttcgg
     2581 gagggtactc agcgacggcg ttgacccatt cctccagtcc ttgcgttctt ccgctggtga
     2641 acgtccggtc gatgagtcct tcttcgacga tcacatgccc catcccgttc aataaggcga
     2701 cattggtgcc cggctccaat tgcagatgca gatcagccag gcgagccaat tcggtccgcc
     2761 gtggatcgat gacgatcaag ggaatccccc gtctgtgggc ctggatgatg cgcgcaccga
     2821 ggaccggatg cgattcggtg ggattggctc cgacaaccag aatcaactta gccagatcga
     2881 aatctttaat tgagttagtg gccgcagacg ctcccagcgt ttccatcatg ccgctgacgg
     2941 tcgcactgtg gcaaacccgc gcgcagttat cgatatgatt cgtcccgatg acgcagcgca
     3001 taaatttgcc gaagagataa ttctcttcat tggtcccccg gctggacgag atcgtcgcca
     3061 gggcgtccgg accatattgg gcggtgatcc gggtcaactc tcctgcgatg cggtccaatg
     3121 cttccgccca cggtataggc ttccatgttc cgccgctccg cagtagtggg gttcttagac
     3181 ggtccggtgc ggtgacatac gtccagccga accgtccttt caagcaggcg tgaccgagat
     3241 tcgatggacc gtcatcggca ggcaccatct gcaccacgtt cccgtcccgc acaccggcgt
     3301 cgaaggcgca gccgacaccg caataggcac aggtggtccg caccgaggtg gcgggcgggc
     3361 cttgcctgta cgcggtcttc tcggtcagcg cccccgtcgg acattctttg acgcaggcgc
     3421 cgcatgagac gcaggtggac gacgcgatgt cgccgctacc ggagataccg ccgcgacccc
     3481 aggcagggcg cgtgccgaag cccgagccaa ccattgaaag agcgaatgcc ggctgaatct
     3541 catcgcaagc tctgacacag cgggcgcacg aaatgcatcg ggcgttatcg aaaataaaaa
     3601 acggatttga ttcgtcgcgt atcggcgcgc gcttcgctcc ggcgggataa cgcacgctcg
     3661 taacgtgata cgcctgcgcc aatttctgga gttcttgagt ggatgctggg tcgagactct
     3721 ctgaaagata cagctcgagc agattcctgc ggtgccgcgt caacgtctca gactccgtcc
     3781 ggacaaccat gccgtcgcgc accggcatcg agcaggttgc atgaaggccg gccaagccga
     3841 caatctcgcc caggcagaga cggcaggatc catatggcga gagatgcgcg gaggcgcaca
     3901 agctgggaat gtggatgccg cctcttacgg ccgccacata aatggtgtca caagattcgg
     3961 cctgaacagt tcgatcgttg atcgtgatct gcatgtctgt agcttcgacg ttcaccgtcg
     4021 cgagccaccc tcgatgtggc cgctcctggc ttgaagtctt tgatgaaatt cgtctccaaa
     4081 atgttcgatg gccgttaaaa cggggtatgg gcctcgggcg ccgtgcgcac aaaggctggt
     4141 ggctttcatc gtgtcgccta gatccgcaat caaagccaga tcggcagcag ttccttgtcc
     4201 attctgaatg cgctccaaca gctcgcggcc tcgcaccgat ccaatgcggc atggcgcgca
     4261 cttcccgcac gactcgtctg cggtaaacgt catataatga cgcgccagat ccaccatatc
     4321 ggtctcatgg tcgaatacaa caatgccgcc gtggccaagg acggcaccgg cttcgtgaaa
     4381 ttcgtcgaag cacagtttga tgtcaagctg tgaggaaggg aagagactgc cgagcggtcc
     4441 ccccacttgg acggccttga attgtgcgcc gtccgccatg ccgccgccaa actgttcgat
     4501 cacctctccg agcgaggcac cgaatggaat ttctaccagg cccggctgtt tgattctgcc
     4561 gctcagttga agagccacgg tacccttgga tcgcgctgtt cccaacgagg catgccaggc
     4621 ggctccacga gaaaagattt cagtgacggt ggcaaacgtc agcacattgc tgacgatggt
     4681 tggtttgcca tagagacccg cgacggcggg atacggaggt ctgaccttga ccattccgcg
     4741 tcccccttcg agcgacgaga gcaaggcagt ttcttctccg cagacatagg acccggctcc
     4801 tcggaacacc tccagctgaa atggcttgtc atcaaccgtc aacagccctc cccgttcaca
     4861 tgcatcgatt gccgcttgta tggtggccgc ggcggcggga tattcccatc gacaataaat
     4921 gtatcctcga tcagcgccaa tggccttcgc acaaatcagc attccttcga tgagccggta
     4981 gggctcgcct tccatgatca tgcgatcaca gtaggtgcct gcatcccctt catctgcatt
     5041 ggccacaaca tatttgcact cggcatgagt gcgacgggcg atgtcccact tttgccagac
     5101 aggaaacgca gcaccgcctc ggccgcgcag ccccgacacc ttcatttcgg cgatgatgcc
     5161 atcgggagat aaccccgccg ctcgttcaat tccacgccag ccgttcgtcg ctcggtaata
     5221 cgccagtgat aagggttccg ccatgccgaa ttgggcaaag acggctcgct gttgtcgcgt
     5281 gaggaacggg acctcagaga tttcgatccc tcccagtcca gccttgatgc gggccagatc
     5341 atcgggttta accagaggcc ataggaggcg gccattgggc ccgtccctct ccacaagcgg
     5401 ttcaagaaaa aacgcgccac ggctcgacgt cctcgtaatc tggatgtcag gatccagact
     5461 ccaagcttca gccaattgat ctgcaccagc cgcgcgtgcc gatgtatcgt tggaaagata
     5521 cagttgggtc gccatgggaa tccgttagcc cggctcttgt gaacaacctt ggcagcgttt
     5581 aaggaggctt cgtaatgccg ccccggtcat acggccgtaa atcatatcat ccaccatcac
     5641 ggtcggtccg actccgcaat tgcccaagca atacacgcgt tccagggtga attg
//
LOCUS       DASSCQ010000124        80072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_887658, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000124 DASSCQ010000000
VERSION     DASSCQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 80072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887658"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFH05_08460"
     CDS             <1..365
                     /locus_tag="VFH05_08460"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HET6678287.1"
                     /translation="VTDPLHERYVIQGDRVTFESQRTGVTRSLSLGEYPALRSFVEAF
                     RMSLMGEAAQIKQLYEVTLDGTPAKWTLLLRPLDPAGKSVVDYLLFTGSDGRIFTIAI
                     RSPDGDRTVMTLLWRTSR"
     gene            362..2680
                     /locus_tag="VFH05_08465"
     CDS             362..2680
                     /locus_tag="VFH05_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET6678288.1"
                     /translation="MRQRYGIGALWVLAMLLGLWLVVTRVVVHSELGDLLPDGTTATQ
                     RLLLTQLRSGQAGRILLLAIEGGNPDELADTSNALREQLQATGHFIFIANGNQVVERR
                     DWDILFRWRYLLSPQVGHESFSSERLRRTLEQRLQDLRSPLAPVIKPTLREDPTGEAL
                     AILAAVSPAETPAAHRGAWMSRDRSKALLVAETRATGFDADAQAKVQQQVRALFESLV
                     GASPFRLRMSGPGVFAVEIKRTIEREAWWLSTTAATLVLVFLYAGYRSVTLVLLSAIP
                     LTSGILIGMLAVQGWFGFIHGITLGFGIALLGVVDDYPIHLFSHLTARGSAGEVIRAI
                     WPTMRLGVLTTTIGFASLLLAGFPALTQLGVFAVVGLMTGAAVTRWILPLFVPEGFVP
                     KYAWAGYASTLQRASRMKPLVPVAVLLASAALLWSHTPIWETDLAQLSPVTERQKQLD
                     HELRQELSAPDVRDVLMIEGPAEEAVLQAGETVMPKIEELRRDGVIAGYDLISRYLPS
                     HRLQQQRQQSLPEPHVLRRNVETALQGLPFRRGVFTPFIDAVEAARSQSPADRTLFEG
                     TALGMRMASLLFEQQGRWIGVVPLRAVEDRQRLKQIVTAWNVPHVGYVDLKEESNRLM
                     TAYRDRTFSVVLWGGVAIAVILAMALKSFKLVGPVFLPILSALIVMVALVNASGESLS
                     VFHVATFLLVMGLGLDYALFFNRSEGSAQERAHTLYGLLVCATTTILVFGILATSTIP
                     VLHAIGLTASVGSFCCLLFTGLMAKEKVHASG"
     gene            2667..3884
                     /locus_tag="VFH05_08470"
     CDS             2667..3884
                     /locus_tag="VFH05_08470"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET6678289.1"
                     /translation="MPPDKRSMTPLTLSAYTLVTANGRGIGAVSRSLRDRRTGLRPCN
                     FEDALLKTYIGRVDGLEDFSLGNGLAPFDCRNNRLAWLALQQDGFMVAVAEAKQRYGS
                     DRIAVVMGTSTSGILETEHAYAQRDPRTDRLGDDFTARYRYTHNMFSSGHFVRACLGL
                     HGPALVISTACSSSAKVFATAARYLQAGLCDAAIVGGVDSLCLTTLYGFSALQLLSAQ
                     PCRPCDEDRDGLSLGEAAGYALLESSANVGRKGAVALLGYGESTDGHHMSQPHPEGLG
                     AIRAIQQSLERAGLVPSDIDYVNLHGTATKANDLVEDKAVHHVFGSAPVVSSTKGWTG
                     HTLGAAGITEALMTAICLKEAFVPGTLHCERVDPALKSRVIRNNEARPIRLAVSNIFG
                     FGGNNCSLVLGVL"
     gene            3884..4675
                     /locus_tag="VFH05_08475"
     CDS             3884..4675
                     /locus_tag="VFH05_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HET6678290.1"
                     /translation="MKVGILGVGLLSAGLDGWMKGRAVLAGTSSYRPGSVPEPESPLL
                     PANERRRSSDAVRWALHVADEAVRQSGLHAQELATVFASSGGEMAVLDQLCRALTTPD
                     RALSPTLFHQSVHNTAAGYWGIATGCQQSSTALASYDDSSALGLLEAVAYATVEERPV
                     LFVAYDLPSPPPLDRARPIRSAFAAALVMTPSASGAPVHADLTLATHTKAEITGMEDQ
                     TLEELRCGNPAARLLPLLRALAQPGSHRASLNVLDDQRLIVEIES"
     gene            4672..5115
                     /locus_tag="VFH05_08480"
     CDS             4672..5115
                     /locus_tag="VFH05_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678291.1"
                     /translation="MTGMLKAEIGLLLPHGESMCLLDAVDSWDERTITCRTSSHRDHR
                     NPLRAGGRLMITAGLEYAAQAMGVHVGLLNGGLRSGKQIGFVGSVRDVTFTINRLDDL
                     TQDLTVTATRLLEGDSSYIYRFSVTHDGSALVEGRASIFIRAASS"
     gene            5112..5834
                     /gene="fabG"
                     /locus_tag="VFH05_08485"
     CDS             5112..5834
                     /gene="fabG"
                     /locus_tag="VFH05_08485"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HET6678292.1"
                     /translation="MKRRALVTGGSGTIGAVICQRLAADGCAVVVHANRHPKSAQQVV
                     ENITADGGAARMTTFDVADPDATRKAIESLLDEGPIQVLVHNAGVHDDAPMAGMTIEQ
                     WKEVLDVSLNGFFHVTQPLLLPMLGTRWGRIVSISSLAGVMGNRGQTNYAAAKSGLHG
                     ASKSLAIELASRGITVNVVAPGVIESPATRHSFSPEQVASIVPMKRAGTPEDVASLVS
                     FLVSEQAGYITGQVIGINGGMA"
     gene            5831..6691
                     /locus_tag="VFH05_08490"
     CDS             5831..6691
                     /locus_tag="VFH05_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6678293.1"
                     /translation="MNQQDHSKPVVLGSTQGGGIHGCERDRCWVVIPAYNEAATVREV
                     ALRSRQECAQVIVVDDGSTDNTAECLAGLDVIVLRNEDNAGKAESLWRGFQRALAHGA
                     VAIITLDADGQHRPEEISRFVAAAQDDPETFFVGARRREHRKISPGRYWANCMADFWI
                     GWAAGVRIEDTQSGFRSYPARLLREVVLRHDRARSFVYESEILIEAARRGFPCRNIAV
                     SAMARPGQRPSNFRPLADALRITKMVSWKLMKRGMYLSGLWCFLTGRSNATQPLSRRA
                     QLARSSPIIQ"
     gene            complement(6714..7124)
                     /locus_tag="VFH05_08495"
     CDS             complement(6714..7124)
                     /locus_tag="VFH05_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678294.1"
                     /translation="MKRIRWATVAACVALVAMIGCRGGGQVYQVKDAPVQTASGKDLS
                     LDEVRKEIVAAGVAAGWQMAPAKSGEIIGTLNIRSHQAVVSIPYTTKTYSILYKDSSN
                     LKYDANAQTIHENYASWIQRLDGAIRSRLAAAGS"
     gene            complement(7153..8016)
                     /locus_tag="VFH05_08500"
     CDS             complement(7153..8016)
                     /locus_tag="VFH05_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET6678295.1"
                     /translation="MKHLHFTIDCDWVPGSEKGLEALLDCCDRYGMTATVFFTGRFAE
                     AYPNLVRDCRERGHQLGTHGWAHGGLEEDEDFRTAGYEQQRTWIRQATQAVEHAAGVR
                     PVIFRAPNLWIGETTLRILEEEGYLYDSSVPARRFDLGFGRVHYLKYFWAPTQPYRPS
                     EADLGKPGTSVITEIPPSAFLFPVNMATLRVLGVQTLGKMIAWIERASRHLVFYCHPS
                     EFIHAKDQSFPKNMSKWNTRGMGPKNLRVLEALLDYLYARHFVPSPLMSSAATHLDVS
                     HPVVIRASAFS"
     gene            complement(8148..9197)
                     /locus_tag="VFH05_08505"
     CDS             complement(8148..9197)
                     /locus_tag="VFH05_08505"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678296.1"
                     /translation="MGMRHHQASSPASAQRVLEPTSHVKISYLEPDELVRQLTTTETY
                     PLAVVSFGLPLPFSLPCPVVHLDLPLLDRSGRCELWSCDQPVRLYKENGVSLAVSGSL
                     LFGSITVAEDPDCGLDRTTEIAYRHLLHQLLESGFPHLWRIWNYFPAINQDQHGLERY
                     RLFCVGRHQALADTLPGFPASLPAGTAVGTQSGPLQIYFLAGAHPALHLGNPRQVNAY
                     DYPSLYGPRSPSFARASLYRSDSATQLFISGTASVVGHQSQHKGLADLQARETMTNLR
                     ALLDRAHCVPATPEREARLQSIFKVYVRNRDHLDAVRRVTDDPFFASSHLLYLQGDLC
                     RKELLVEIEGLVTAD"
     gene            complement(9211..10563)
                     /locus_tag="VFH05_08510"
     CDS             complement(9211..10563)
                     /locus_tag="VFH05_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HET6678297.1"
                     /translation="MNSLSSIVAPCICCDVLVIGGGPAGSTISALLAEQGWNVHVLEK
                     DSHPRFHIGESLLPQSVPMLRRLGVLPDVEKIGIVKYGAELVSHRFGRSQMFHFAKAY
                     DDSQPYAFEVKRAEFDAILARNATQKGARFHEGVRAQRAEFRPGRTSLVYAEDRTGHA
                     RTWEARFIVDASGRDTFLSGQLSGKHRSKKHGSAAVFSHFEGVTRLTGLDEGNITAAW
                     LDDGWCWMIPFKDGTMSVGVVCHPDYLKSRRTPLDQFLLDTLRQSPPIAARMERARPL
                     TPAYAAANFSYRRDTMSGDGYLMIGDAFAFIDPVFSSGVHLALNSGTLGAGVVDAFLR
                     GSPEYAVRRKHFERIVRRGIDTFSWFIHRFNQPAFQALFVSPKRPAKIERAVLSLLAG
                     DVFAQSKMRFPLFLFKVVYYVVFMLNWKENWSVARRRKRGSRTTVTEVTDYAVKSASA
                     "
     gene            10760..11086
                     /locus_tag="VFH05_08515"
     CDS             10760..11086
                     /locus_tag="VFH05_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678298.1"
                     /translation="MRLVGMVLFAALLIGSAACHSTHVQIPTAPIAPGEKSLGPTWGD
                     HVGLMLFGFIPIQQNDRFVEAYNEAVQKAGATRLTNVIISERWWWGYVINGYVFKVEG
                     TAVTNK"
     gene            11110..11430
                     /locus_tag="VFH05_08520"
     CDS             11110..11430
                     /locus_tag="VFH05_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678299.1"
                     /translation="MKAAGVLLGMCLLFTACVSTPLKFPSAPIGPNEKSLGKTEATST
                     GIMLFGFIPINQNTRFEDAMREAAAKAGGTRLTDVTISEQWFYAVVLDGFIFKVEGTA
                     VANK"
     gene            11620..12384
                     /locus_tag="VFH05_08525"
     CDS             11620..12384
                     /locus_tag="VFH05_08525"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6678300.1"
                     /translation="MNILDQFFVFHPERWHEKDWRSASGLPLEDVWFKAADGATLFGW
                     YVEAPADRPVVIWCHGNAGNIVNRLDNLRFLFRQNLSVFLFDYRGYGKSQNVRPSEEG
                     LYQDAIGAYEYLVRGRRLKPERLIIFGRSLGAAVAADLASHKPAAGLILESAFPSIEA
                     VAKHYYGGLPVHWLLGAQFRLIERLPALSLPKLIIHGDRDDIIPLELGRQIYEAAKPP
                     KMFHVINGAAHNNTYQVGGTRYFQTFSDFAGRAIRS"
     gene            12499..13425
                     /locus_tag="VFH05_08530"
     CDS             12499..13425
                     /locus_tag="VFH05_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HET6678301.1"
                     /translation="MRISWPRVILSVLSLHVLLTGCVAVATTEQAEESYSSTAHNGPA
                     SLLLVADLGDGAVSDVSVAASQSQGSDGTGEDAYDPFAKSGEETEQEYDPWESLNTKV
                     FEFNRQVDRYVLKPVAQGYDFIMPDFVQVGISNMFYNLRFPQRFLNNVFQGKVKGAGI
                     EVGRFLINSAVGYAGFLDLAKEVDLVTPEEDFGQTLGSYGVKPGPYLVIPFLQPFTAR
                     DFTGYVVDIFLNPINWLVAPIIEVEGVPSLIAHKNRTTTTFVQIGARVGEIVNERSRN
                     LEKFQGVEEATLDLYTAVRNAYLQSRARAIQE"
     gene            complement(13435..14631)
                     /locus_tag="VFH05_08535"
     CDS             complement(13435..14631)
                     /locus_tag="VFH05_08535"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HET6678302.1"
                     /translation="MADSTLKQIVSEQLASIRAKGLYKSERQLLSPQNTDISVAQGRV
                     LNLCANNYLGLANHPRIIEAAIKGLRDHGYGMASVRFICGTQDIHKELERAISTFLGT
                     DDAILYGSCFDANGGLFEVLLDERDAVISDALNHASVIDGIRLCKAKRFRYGHGDMKE
                     LEARLHETRPFRLRLVATDGVFSMDGDLAKLDHIVELAERFDAAVMVDDSHATGVIGR
                     RGRGTPEHFGVAERIDIVTSTLGKTLGGATGGFTAGCAGVIELLRQRSRPYLFSNSLP
                     PSIAAGALQALALVAEGDSLRARLSENAAFFRARLTELGFRLIPGDHPIIPVLLGEAS
                     LATSMADRLLEEYVYVVGFSYPVVPEGQARIRVQMSAAHTREQLEHAVEAFAKVGQEL
                     GVISKR"
     gene            complement(14673..15695)
                     /gene="tdh"
                     /locus_tag="VFH05_08540"
     CDS             complement(14673..15695)
                     /gene="tdh"
                     /locus_tag="VFH05_08540"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HET6678303.1"
                     /translation="MKTAPQPGLTVMNCPDPAPGPHEAVIRVKATSLCGTDAHIYNWD
                     PWAHSRIHPPRIIGHEMCGEVVEVGPAVKLVKVGDYVAAESHITCGLCFQCRTGQAHV
                     CRNYKILGVDRDGSFADFVVLPENVLWHTSRAIAPEYASLQEPLGNAVDAALVEDLTG
                     HTVLITGCGPTGLFAAAVARTSGAATIIASDISEYRLGLAKQVGADYVLNAKTETAAS
                     LVKAIDEITDGEGVDASLEMSGDPAALHLAFDAVKNGGRVTLFGIPTGPITCDLANDI
                     IFKGIRVHGITGRRLFGTWYRLAGLFKAGLNIKPIVTHTFPMSDFAKGFDLIKTGQCG
                     KVVLFP"
     gene            15799..16782
                     /locus_tag="VFH05_08545"
     CDS             15799..16782
                     /locus_tag="VFH05_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET6678304.1"
                     /translation="MCAAPHHRRVMLRIGAFGVAVFLCGALLTAISPSSAADAGRRWA
                     RFELNQQDASGDLQTGKPVALVITLGGVAQGTGPIVALCESILFEPQLITLEPDGESS
                     ALKAEVTLDPMPSSRTSVHQKAARIQVTFARARPEKLERFMKRIVYVTLEREETSSDT
                     SEPPVVRPEEPSTGDLIVDEPQPSGIAPVSTGALLEEDLMPLGDPGQGKAYWQQVSLL
                     VSRSWARQVRGVRRSPSSETVRVRFKLYPNGRAQLIQIEKGSGAREIDEAGIYAVVNA
                     QPFPPLPEEVGDEPVDVHVRMRTGARSTAREVQSVGNSSNGKLETPAQPKK"
     gene            16989..17990
                     /locus_tag="VFH05_08550"
     CDS             16989..17990
                     /locus_tag="VFH05_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET6678305.1"
                     /translation="MKAMVLDSPGDVAGNPLRLDNVPMPEPGSSEVLVRVSVCGICRT
                     DLHVVEGELERPKLPLIPGHQAVGLITRLGSGVNERTIGERVGVAWLQGTCGQCDYCR
                     SGRENLCLSARFTGYQVDGGYAEYVVLPAAFAYPIPPAFSDEEAAPLLCAGIIGYRAL
                     RVSHIQPGQRLGLYGFGASAHIAIQLARHWGCEVYVCSLKPSHQALARALGAAWVGGA
                     SEAPPVRLHASIMFAPAGELVIPALRALERGGTLALAGIHMSTIPPLEYDRDLFGERV
                     IRSVTANTRQDGFDLLREAAAIPIRPRTQRFSLDQANHALQALKSGAVNGAGVLAIH"
     gene            18042..18626
                     /locus_tag="VFH05_08555"
     CDS             18042..18626
                     /locus_tag="VFH05_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678306.1"
                     /translation="MRRSVWFVLGLTLVGWGTPAASALEFTADQITKIDGRTHKAFIY
                     YRDQMWRIEHHSLGPVNVTIVRKDKHLVWLLLSRMKHFKTLPYDVDQDLRVTARLDGE
                     ISRQEIGTEIREGHPTTLYEVTTKQGEHVEHYYQWVATDLQFPMKLAKKDGSWIVEYQ
                     HVKMRSLSDYLFNLPVNFQPLEEFDASPPVSKEQ"
     gene            18647..19588
                     /locus_tag="VFH05_08560"
     CDS             18647..19588
                     /locus_tag="VFH05_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET6678307.1"
                     /translation="MRRAKLIGVMVAAMLMGTSGWVSALDVADVTREWTADGKKIAAE
                     RAKLPAHDEMVKIPAGTFLMGSDKKVDRNAYQPEFPQRRVYLDAYEIDKYEVTTVQFL
                     KFVLATDRKPLIDWQYEGGNFQETMANHPVMHVSWFDADAYCRWAGKRLPTSAEWEKA
                     ARGEDGRIYPWGNEPAGLSRANFGRTGLSGPVRDRPERLLLYPPIISVDKYDNGVSPY
                     GVFQLAGNVAEWTADWYDPHYYKTAPDRNPKGPEKGTQRAFRGGGWIDSTPSVRPAQR
                     NGTDPNTKMNWLGFRCARDAKDAGDASDGKPAQTSLQ"
     gene            complement(19601..20875)
                     /locus_tag="VFH05_08565"
     CDS             complement(19601..20875)
                     /locus_tag="VFH05_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET6678308.1"
                     /translation="MTGSEQTDQLRNKTKQFAIGILKLCGSLPKTPETLAIQSPLVKA
                     ATTLAANCRALARAWSEDDFHRNRNLASGDADDCVFWLELLAAAGTDQDQSIQALLDE
                     ASDIVALLSICGEPDNSAQARTEPVESAAEPPTPSPPVIPTDFHGLRVLSFESRMGVE
                     MARLIQRHNGHPLVVSALREIPIPLQDNSAVFRFGVKLILNQLDILILMTGVGTKALL
                     EILQTRYPLAEIIDALKKTIIVTRGPKPLAILKTLGVEANITVPEPNTWHDVIATLDY
                     YRPVQGLKIAIQEYGVSKPDMIQDLQKRGADVFSVPVYRWALPEDTAPLHSAITEVVN
                     GRIDAMVITNAAQVDHVMQLAEQDQKTELFRESCKKIVVASIGPTASEGLKHYDLPVD
                     FEPSHPKMGVLVKELSERIHALRQAKLPRQPD"
     gene            complement(20920..21735)
                     /locus_tag="VFH05_08570"
     CDS             complement(20920..21735)
                     /locus_tag="VFH05_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET6678309.1"
                     /translation="MPSLLPFVIIGVILLAPCDLRAENSQLGEGASAPMVLIPAGPFP
                     MGVPLGDRDGGRDEYPRHEVYLDAYMIDTYEVTNGRYLEFVKATGHRVPQNPINPTRN
                     LWEGDRITESIADRPVVNVDWTDADAYCRWTGKRLPTEAEWEKAAKGNHDWRFPWGNI
                     EPTTKHLNYNQRWIGEKTLMPVGSYEAGKSPYGLYDMAGNVWEWVQDWYDPRYYEKSP
                     KNNPKGPETGTKRVLRGSGWQNETPTVRIFTRVDSDPTVRNESTGFRCARDAK"
     gene            21870..22289
                     /locus_tag="VFH05_08575"
     CDS             21870..22289
                     /locus_tag="VFH05_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6678310.1"
                     /translation="MRRAVHKGLRHVALRVTNLARSRAFYEALLGMQVVWEPDADNVY
                     LSSGTDNLALHQISAAELDAYRPPQAQLLDHMGVILEDREAVDGMYREIETKVESMGG
                     RIAKAPKQHRDGSYSFYFSDPDGNLIQALYEPTISQT"
     gene            22286..23920
                     /locus_tag="VFH05_08580"
     CDS             22286..23920
                     /locus_tag="VFH05_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6678311.1"
                     /translation="MIMNTIWDSVSPDHYLSLAPWCWVQLESADPPGPFPFVAGLAPE
                     VVGALHEAHSLLASSIDTAISDVFAKRAPLDDPLRSRRLEDAYAELVNARPYLSRHIK
                     CGRTVDGRFQWDIPFDPTKSATVTNGGLRIFHAVKRQAIPIGFDQRSLGPIVGRLLGM
                     LDGTHRMEEIKTVVRSSPREAQQVLSRLVDSLHQYECLRSSSEASIGRRWFDVIQDRD
                     TVHLGHAALLYRQRDDAFLFDPWLLPWFAESSVPSLWTSLLPKPAAIFLTHDHDDHVD
                     PRTLLHLPKDTPVVVPSRKNRHRLYYDYPSLLRELGFTRVVELAHGEAWPFEGGTVLS
                     VPFYGEDPCDLQMPRNCYLISDRGHNVLVHADSGPTNDGRSALKDHVIARLVEKYGPI
                     DLVFASQQQLLEIRSQAAHAALSHPGKWLDVGENGYLTNDYLADLCATAKARLFVSYA
                     TGGADWYPDHLSFMFSTRNPARTALLTAHWEPPENLKALVTPHGCGYHYARALDVFRE
                     TGGRIEVQPAGEDLMPLHLYRAVHGEPPFMKQGARP"
     gene            23951..24964
                     /locus_tag="VFH05_08585"
     CDS             23951..24964
                     /locus_tag="VFH05_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HET6678312.1"
                     /translation="MRSLTLVTGVAGFIGFHVARRLIDRGDHIIGLDNVNDYYDVRLK
                     EARLAQLTALNGFQFVKLDLANRQGMKEFFSEYPIRRVVHLAAQAGVRYSLINPHAYT
                     DSNIEGFMNILEGCRQLKTEHLVYASSSSVYGGNTHMPFSIHDNVDHPISLYAASKKA
                     NELMAHCYAHLYEIPCTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIEVFNHGKMQR
                     DFTYIDDIVEGVIRTLDHPAQPNPSWSSDRPDAGTSLAPARIYNIGNHQPVELLHFIE
                     VLEGALGKKADMTLLPMQPGDVPATYADIDDLAHDVGFSPSTSIEVGIPRFVEWYRSF
                     YKV"
     gene            complement(25004..25579)
                     /locus_tag="VFH05_08590"
     CDS             complement(25004..25579)
                     /locus_tag="VFH05_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678313.1"
                     /translation="MRLSTWFSLVLGTMLAMLASQTLAASPDSPSFDPARMWECTQAD
                     GSSIYTNKERTGCTLMTLKELSVVPSLDQMPTYRPAPTTHEDMAAYGDRGRRAVTGDQ
                     RHVPDWARDWHATVASTGVVQAEVCALYGEWLNLNQKSRGGFYFGSDPSYGGDLSGQN
                     QRGASFSFYDNARYLSLAKMFGAGFVPIGCL"
     gene            complement(25661..26401)
                     /locus_tag="VFH05_08595"
     CDS             complement(25661..26401)
                     /locus_tag="VFH05_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET6678314.1"
                     /translation="MIGAMVFDFNGVIVDDETPHLLCFQQALAEAGLTLTTQDYYGAY
                     LGMDERTCTRLLLEQRDGMCDEARILRITDRKADLFRAHSAHHRPPLFAGAADFVLAA
                     QGRYRLAIASGGRRAQIEDALAGSAIEQAFEVMVTAEDCPIGKPDPAIYLLACDRLNA
                     NRGSAERITPSQCLVIEDSLAGIRSARAAGMRVLAVSTTYPLEMLNEAELVFRGLHDM
                     TPQTAIDALALASPRCQPAAFSTPSKDE"
     gene            26580..28580
                     /locus_tag="VFH05_08600"
     CDS             26580..28580
                     /locus_tag="VFH05_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET6678315.1"
                     /translation="MTDYGVLGNLLVIFSVSIAVVFMFHQFRLPSIAGFLVAGALIGP
                     HGLNLIADIGTVQVLAEIGVVLLLFTIGIEFSLVQLASLRRLLFMAGPIQVGTVLAVA
                     WVAAMSVGMTWQQGIFWGFLLSLSSTAIVLKTLAERGDSDSIHGRTTIGILIFQDLAV
                     VPMMLLMPILATRAEGSLTTILFTLAESVFVVGLIIAAAWYAVPKLLEHIVRSRSREL
                     FLLTVIVLCLGIAWLTSLGGLSLALGAFIAGLVISESEYSHQAMADVLPFRDSFNSLF
                     FVSIGILMDWRVLIDHPVLVFGLLLAILFVKFLAGAAASFVLQIPPRSAIMVGVALAQ
                     VGEFSFLLAQQAQESGLLRGDPYQVFLSVSVLSMIVTPFLMQWSPAMARRAEAIQRLR
                     RWLPTRTEAHVLQTQGSHIRLKDHVIIVGYGLNGRNLARVLSETEIPHLALDLDGDTV
                     RRESRHGVPIYYGDATNPNVLRHVKIEEAKVLVVAISDPFITRRTVQVAKVLNPKIRV
                     VVRTRYLRELEELHDLGADDVVPEEFETSIEIFALVLRTYNLPQDFITMKAEQVRREG
                     YALLRRSELPELAHHLRGGTLTDAEVETCRIDDESPAVGKTFADLSIRPRTGASVIAW
                     TRNGITESNPSLKTKLKSGDILVLLGSRPQIRRAMSLLLSGE"
     gene            28707..29243
                     /locus_tag="VFH05_08605"
     CDS             28707..29243
                     /locus_tag="VFH05_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HET6678316.1"
                     /translation="MTTTTPQMDPAVALADLQASKPDKVTIVLLSGDLDRAMAAFIIA
                     TGAAAMGMQVTMFFTFWGLNTIRRKGASSSAKDWLRRMFGVLNKGGAESLPLSRFHFG
                     GIGTSMMHKVMKQNRMPGVPELMQTAIDLGVRFIACTTTMGLMGISKDTLIDGIDQFA
                     GVTTYLAEAKQGNVNLFI"
     gene            29303..29545
                     /locus_tag="VFH05_08610"
     CDS             29303..29545
                     /locus_tag="VFH05_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET6678317.1"
                     /translation="MKADVKLDTLGYFCPMPIIMTSKKIKELTLGQVLEVVSDDEGIK
                     KDMPAWCDTTGHHLMGLEEEQATSGPIYKAFVKKTK"
     gene            29632..31293
                     /gene="ftcD"
                     /locus_tag="VFH05_08615"
     CDS             29632..31293
                     /gene="ftcD"
                     /locus_tag="VFH05_08615"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET6678318.1"
                     /translation="MDRIVECVPNFSEGRSQATVQALIDAIASVPGVRLLNHTSDRDH
                     HRSVLTVVGDLEEMVEAMFRSVRVATDLIDLRRHSGVHPRVGATDVVPFVPIKGLSMR
                     DCVQAAKRLGHRVGTELEIPVFLYERAASHRDHASLESVRRGGLDGLAFRMATDPDWS
                     PDFGPSQLHETAGAIVIGARPPLVAFNVNLLSSDVNVARSIAGKIRQSNGGIPHLKAI
                     GVELASRGIVQVAMNLTDYEAIPLHAAFAAVQSQAAERGVSVAGTEIVGLVPLAALVE
                     TAKEALRLDHFEPSQLLEAQIEAAWSRGEKAKGSRTTVAQPFRLPVPRFIEAVAAPTA
                     VPGGGAVAALVGALASALGMMGAGLSRQPATERRLKDIVERLSHLLQADGEAYRTFVE
                     ATRRPKTDEQRSMAVSSALHVATEIPLEIAEQSAKAGTLLHACSCGVKPRLQSDMQVG
                     LILAIAAAEAGLHTAGENLKIQPNHKLRGSLAARVQRLSQNLEELKVLCYTPPPGQSG
                     KHSAQALPGKVRTRDEWKLKSSITTSRKRSKLRRKSLPEKDSFEN"
     gene            31203..31397
                     /gene="rpsU"
                     /locus_tag="VFH05_08620"
     CDS             31203..31397
                     /gene="rpsU"
                     /locus_tag="VFH05_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET6678319.1"
                     /translation="MEIKVFNNNVEKALKVAKKKLAGEGLFRELKRRRYYEKPSVRKK
                     AKQREAQRRRQKWLSKRRPE"
     gene            31452..32111
                     /gene="nth"
                     /locus_tag="VFH05_08625"
     CDS             31452..32111
                     /gene="nth"
                     /locus_tag="VFH05_08625"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET6678320.1"
                     /translation="MRQDQIHEAIRILKREVRQWQEPVVGVIARTSARDPFRILISCL
                     LSLRTKDKTTSEASARLFALAYTPEGLLNLPRRTIERVIYPVGFYRTKAKAIHAICRR
                     LLEVYGGTVPDSIDELVTLPGVGRKTANLVVTVGYNKPGICVDIHVHRISNRWGYVKT
                     GKPEETEQALRHKLPKRHWIIFNDLLVPYGQNICQPVSPFCSRCKLAEFCDRVGVVRS
                     R"
     gene            complement(32141..32563)
                     /locus_tag="VFH05_08630"
     CDS             complement(32141..32563)
                     /locus_tag="VFH05_08630"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HET6678321.1"
                     /translation="MSPPSPKFVLFAHNATYDKLHQVATLGLTAAAMGKDVIVILLFW
                     TIKKLAEGKLDQIDFPPEYSGSAEIVGRLLKEKHVPTISQMFEEAKLVGKVRIIACSA
                     GLEYMGVDGEAVSKQVDEVMGLPAILSLTADAETKLFI"
     gene            complement(32566..32892)
                     /locus_tag="VFH05_08635"
     CDS             complement(32566..32892)
                     /locus_tag="VFH05_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678322.1"
                     /translation="MFTTRLCCVLLWLAVCTSPVLSAGSDDLVIAINKKGLGKEVVIT
                     QGLREWFMLVEVTPENTVIVRQEKERDTYLLDESETHDRPLAPTEVDAAINDYINSVK
                     ARVPKP"
     gene            33017..33448
                     /locus_tag="VFH05_08640"
     CDS             33017..33448
                     /locus_tag="VFH05_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HET6678323.1"
                     /translation="MDWLLSSVQHYVSTETLIWLTVLSIIFFVGTLIAIPFILVRLPA
                     DYFDVRVPRPWMQDHHPLLRVVGHIVKNALGAVFVFAGFLMLFLPGQGILTMLIGISM
                     LDFPGKRNIEAKLIGQPTVLGVINGMRKRRGKPPLILAPDS"
     gene            33487..36144
                     /gene="polA"
                     /locus_tag="VFH05_08645"
     CDS             33487..36144
                     /gene="polA"
                     /locus_tag="VFH05_08645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET6678324.1"
                     /translation="MSEAAVSSRPTLYLIDGSAYIYRAFFALPPLNNSKGLQTNAVYG
                     FTTTLMKIIREHQPQGLAVAFDEPGPTLRHETYKAYKAQRPPMPEGMSGQIPYIHRIV
                     DAMNIPVVKLAGYEADDLIGTLACQAERAGYDVVIVTGDKDMFQLLAQHIRIYDPVKS
                     KWFGEAECRARFGVEPARVVEVMGLMGDSTDNIPGVRGIGEKTAIKLITEFGTIDELL
                     RRLDDVTPPRIKTLLVEQADNARLSRTLATIDTQSPVEFQADTYRVKSLHHDVFADLL
                     RELEFTTLLKNLQPAERHAEPKAVSVVSLEDHPSIHRFIGELPPGVPLGLQCLLAGGS
                     GMQAEVLGLALSVGDSTGFVPLKSGPLENSLAALLRDSGRQKVVHDLKAALLACSRAG
                     LILNGPYVDTMVADYLLNPNRRDHQLETIALEVLGERLEKTSEEKSKQSLFEVELASR
                     EDVAQSAAALARLGPVMTQRIAEQGSAKLFAEVEMPLVPVLVEIERNGFLLDVEKLHA
                     LGKELDRELERMMDTIAGLAGGEFNINSPKQLAIVLFEKLGLKPVRKTKTGYSTDEDT
                     LTQLAAQHELPSQILNCRSLSKLKSTYVDALPQLVNPHTRRLHTSLNQTVAATGRLSS
                     TEPNLQNIPVKGDFGLRIREAFVAADGHALLCADYSQIEPRILAHLSQDPRLLAIFAQ
                     SEDIHLATAMEIFSLPADQITKEMRRAAKTVVFGIVYGISPFGLSQNIGVPQAEAKKY
                     IETFFERFPAVRALMDRNIAEGKAKGYTTTILGRRRPIPELQSGDPVQRSFGERMAVN
                     SPIQGSAADLIKVAMIRVHQALQEQLPQTKIILQVHDELIFEVPEANLEAAKRLVKTE
                     MEAVGQTLGLSVPLKVDLGVGKNWRLAHP"
     gene            36320..37180
                     /locus_tag="VFH05_08650"
     CDS             36320..37180
                     /locus_tag="VFH05_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678325.1"
                     /translation="MIHAVELDSQTSSFVRQLEEQRRIEKNLMVERVRGSKEGLESVF
                     HDIKERVDALERMTNSVSTSYIAPIGWSQLIFAGTDGLSAGPTMGFGDRDDSICKHDR
                     IRYLEDANYLRLSANPHYVERVLPNKSAHARAAANPSTWDNLGYVHVGYSTALILPAS
                     LEPTTVSILSRVNTLFNWVEVNAVADDWYAHVAATARLWVAYDGEFRQTPPAQFVNLL
                     ATPKQVKTHQHIDSLAPSAALAMQLEVSPGSGTTLMMYETIELVATTPNAHAYIDGVF
                     AWEPVAVQLR"
     gene            complement(37199..38464)
                     /locus_tag="VFH05_08655"
     CDS             complement(37199..38464)
                     /locus_tag="VFH05_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678326.1"
                     /translation="MPIENNFQHDELSRKHPGERLAAREFTAGPQPDSPEWTAEMVKL
                     GSAMGRAGVRTVMFVHGAIHGTDVFGTQRLDEAGGLKRGYSRGVSGLDALLAAMRQET
                     NGIPLLPGGLRPPLINDDATKQLIDHHAGDAGNFTETAIGMLQRALNKASTPLRCVRL
                     LWSSEHHHLGRAVAAVRMLDELRMHCELHQLASGDRILIQAHGQAGLVLALASNLLCP
                     SAITGRPRLLAILTSYAEQTNQPDLAAVVNRLAPLLSAGSLLNGASLDIVTFGQPVRY
                     GWDPSGIGKLLHIVNHRNLRTDAKTWLAKMELPQITMEMPIAWGGDYVQELAVAGSDA
                     LPATEQAKAANKAAWELVEPFDGFERWLECARRAIRCPSEGRCLLVDYKDSTGSTNPR
                     DHYYGHAAYTRRDAMLFNTGQIVDTFYQS"
     gene            complement(38514..38741)
                     /locus_tag="VFH05_08660"
     CDS             complement(38514..38741)
                     /locus_tag="VFH05_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678327.1"
                     /translation="MIDRVRERSGRGVDQDIELTREEIVQEYLQGVKIVDRRIDVERN
                     ICSATAVMPKRQILQPKPLIERQESTPVITR"
     gene            complement(39075..40289)
                     /locus_tag="VFH05_08665"
     CDS             complement(39075..40289)
                     /locus_tag="VFH05_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678328.1"
                     /translation="MGARDTQEDAMRLATEAAKRNALEQVATYLESITVVEGVDVTRD
                     EITTYTAGLVVVHDQQTTLSLDGDAVIITVDLTSQIDTEEVAQAILALRENEDARQQL
                     AALKRDIDDLHQELEVAAQSLAVALSAEQAQQASLQRQELLDRVQSHAMVSQAWTDWV
                     MIGPATYPSQLFGITPSHMLLTKARELYPGSPHVPTAQRVMVASTPAAATQTIVPTTH
                     VIPVQPGARTTPLPLNEISHRTPTMPVQLGNQPGGITLPTRVGTRTFTDMRQLAPLSS
                     PPSGNAPAGGQPRPPALPSVQSLQQFLQAPTAIAPSRGSRDLRQGSRSIQSLRQFLQP
                     PATVPPTGQPPVTRAVPTSPHHIQSPLLVPHLPHYGVPRVSEGPGRYGRGGRGGNGFG
                     RAGGGRAGGKGR"
     gene            40621..41640
                     /locus_tag="VFH05_08670"
     CDS             40621..41640
                     /locus_tag="VFH05_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6678329.1"
                     /translation="MRWKKSWVGALTMSLCLVSIMVSSAAAPLRPSSREGNAIPTSPP
                     TEHVILFVLEGFSQDSLKGGTMPTLSKLVKEGAVTWSASAVKPALRLPTMASIITGVP
                     VEKHGIHWNTFEFSRGYPRAPSVFDYLDLSGGRDSAIFFMDESLYQLARPEPYTDYQM
                     CGPLKPECSPDRVVSYIQQYFKKATSGHGYGHAILSLPHFLVVHLPEAGRVGAARGWT
                     SKEYREALRAVDKAMASVLDIYKSEDLTKRTTVFVTSLSAEGIDAGHEQATNGTAPVV
                     PWIASGVGIKHGHAIHQHVSILDTGATVMRTLGLQTHTEWESRPVEEIFQFAFIAAAP
                     NSNVP"
     gene            41651..42100
                     /locus_tag="VFH05_08675"
     CDS             41651..42100
                     /locus_tag="VFH05_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678330.1"
                     /translation="MDSPGGLDAWRTLLVSVSLFLCAGLVSLPVSEAAPPPSYTKEHA
                     IMIDPTVLDPPSWWYVPGITPSIYNSDPDSLDAYKTTDKRTLALKPGKYKFISFTFDF
                     PFSVNLEGKLDFAPSLDQCVEGRGTQTLVVRCKRTYPYGGQPDYTYQ"
     gene            42199..43557
                     /locus_tag="VFH05_08680"
     CDS             42199..43557
                     /locus_tag="VFH05_08680"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6678331.1"
                     /translation="MTQALDDLLEALEDVDDATREEAAKALGTMADPKSLDALIGACE
                     DEYWAVRAHAGSALAKIGGQRAADALVGLFNDPIMEVRNQAVEAAATLGVGVLDRMIT
                     ALKDERWRVREHAAKTCGDIGDRKAVDALIVACRDRDGAVKSAAAEALGKIGDPRAVP
                     VMIKLFRDTSKIVRETAGTALVYIGQPSVDPLIECFKDKDFVVRCHAARALGGMTTDY
                     QIGRTWVREPKVVDALIAALKDPDRAVREDATIALGMIGDPRAIDALIEAMKDGAVKR
                     HAIASLGMIGDPRALGPVLDALKGKGIRQDGTPTPGCIVSEDAFIKEAAATALGQFRD
                     PRVIPDLIMLLKDGVLREKASAALAAIGDTAIEPLIAFLYDPKASEVSAEGERVLSYA
                     SVRLSAKDALRQLVMETLEKLGWTLPQEDVAVNSSSVDNARVDKPLGEAGRFGPAGDL
                     AK"
     gene            43954..45036
                     /locus_tag="VFH05_08685"
     CDS             43954..45036
                     /locus_tag="VFH05_08685"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6678332.1"
                     /translation="MADSVAEQIAALQDDDWAIREEAAVALGAFRDPRAVGPLVGLLR
                     DSDRAVREAAIGALTAIGGPSVPVLGLCLSDPQLGVQEAASSVLASIADERVIDPLIG
                     ALANSDWIVRMHATKALGRIKAAGAIDPLIPLLQDKVKAVREEATVALAKIGDAALSS
                     LLDALKHPEWLVRLHAVEALARMRSPRAVEPLLWVLFNDTDQAIREDVIRALGQIGDS
                     RATEFLVTAMKEPRLRTLAVESLGQIGDRSAVPLLVAVLEGADRLEGLRPVAGCGDRW
                     DEEMVTKGAAVRALGSIGDDTAIPALLKALDQTETRADAAAALVKFGSKVIAPLLTIM
                     TQSSDENVRFHVKDTLAQVGWRAGRI"
     gene            45115..46212
                     /locus_tag="VFH05_08690"
     CDS             45115..46212
                     /locus_tag="VFH05_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6678333.1"
                     /translation="MSKETIETLVAELVHEEDWRRMRATAACLAGGPRAVQALIEVLR
                     SGTSDLKREAAAMLARIKDPHAGVALVGLLEDHDEGVRRAGATALEQMAGVLDPDTAA
                     ALVALLPKTAEGDTRRLLSHLVGAIPTAVAPLCEMMKQGDPEAQVTAATMLDHLLDPR
                     SVDAFIDAMAHPAVRDIAVWTLKKLGAIRERIDDSFNALRDVEGASEREEARMATVIN
                     LLGIGRPSVEILIEYLEDDDWLVREAAADLLGKIADVRAVEPLMKRLEQDKDTGVKEL
                     AIKALGLIGDARPTRLYLEAIPIRPLRVYAMEALAKIKDVEVLRPDKELFDRLRTDRD
                     GLVAYNAGLIADKLEAIDSAHAISREGHGDE"
     gene            46205..46972
                     /locus_tag="VFH05_08695"
     CDS             46205..46972
                     /locus_tag="VFH05_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6678334.1"
                     /translation="MSDEGRSERVEQLIGALRDDNEALRDHAIASLSQVGEEAIGPLI
                     GLMADEDVLIREAATVAIVRMGPSVVDRLIEAIEDDEWAIREQAASGLGKLKDPRAVE
                     PLVKALKDKDGAVRTAAVWALERIGDARAVPGLVEALTDGTVREDVARVLKKIGDARA
                     VDALIDGLLGNNWMVRRHAAEALGKIGDARGIEPLIESLRDEDWLVRRNAAESLARLG
                     AKQAIDPLLPLLEDENTMVQETVEGVLASLGWKQGDQ"
     gene            47009..47728
                     /locus_tag="VFH05_08700"
     CDS             47009..47728
                     /locus_tag="VFH05_08700"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6678335.1"
                     /translation="MADEAPVKLIQIGPKGGAKKDGFNLVTERVVAVNPEAKQVEVEL
                     LAYDGKTVVLDVDEAALEELQRLKAGDGATIRVVEEGGKRIAKSFRIRAKDPDAAKAD
                     AMLLDLKDSHWLNRKYAAEVLGELREPRAVRPLVDALTDEVGDVRQRAYDSLIKIGGV
                     AVPTLVPLLVSEEDELRQSATEIIRKIGKPAVEPLATALAEADDRLKARVLKVLDRMG
                     YKPKTKDAVKAETPRLTQSPG"
     gene            complement(47725..49041)
                     /locus_tag="VFH05_08705"
     CDS             complement(47725..49041)
                     /locus_tag="VFH05_08705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET6678336.1"
                     /translation="MQISVIGTGYVGLVTGACFAEFGVNVLCMDNDAKRIARLEKGDV
                     PFYEPGITELVAKGIGQGRLNFTAELTRAVDHGEVIFIAVGTPPKKDGSADLSFVEEV
                     GRGIAGKMASYKVVVTKSTVPVGTGAFLQQVIAGTQTRKVAFDVVSNPEFLREGSAIE
                     DFMRPNRVVIGSDSERAVALMKDLYRPLYLIETPFVVTDVATAEMIKYASNAFLATKV
                     SFINEIATLCERVGADVQTVAKGMGLDQRIGSKFLHAGPGFGGSCFPKDLAALVQMGE
                     KAGYPMQIAGAAAAVNAEQRVRMVDKIRDALGGLEGTTVGFLGLSFKPNTNDLREAPA
                     LSIAQLLMDQGVTVRAYDPAALEEGSKLLPRITPCQNAYETARGADAIVLMTEWNEFR
                     NLDFDQLKSVMRRAILIDLRNVYESSRVTQFGFRHVSVGRPTKNPG"
     gene            49269..50810
                     /locus_tag="VFH05_08710"
     CDS             49269..50810
                     /locus_tag="VFH05_08710"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6678337.1"
                     /translation="MHHPSWFLFLTAFITGAVVMALEILGSRLLAPVFGNSLFVWGAL
                     IGVILAAMSSGYAFGGWISDRLPGGHVLAGLLLFSGSWTFLIAWASQPILFKVAGVIE
                     DPRWGPCLAATVLLAPPAFGLSGVLPAMLRLAVSDLNYLGRHTGRMIALSTVGSLAGT
                     WGTAFFFLSWIGSQALVAWLGVMQTALGLWWLVKGTAAKRVTVVCFAMIIGGLAVGAW
                     SPIHMLKPPVYQEDSPYQQVRIREDDLFRYLVLDRTFHAVMWKAEPVTLFLPYSQLMV
                     SSLALVPEAKRALVLGHGGGSFAKWLARYWPDLDLDVVEFDPVVVRMAEDYFSYRPPS
                     RHHVYVRDARAYLNDTANTYDLIWVDAFARHLVPFHLTTQEFFSDLRKHLTPEGVVVV
                     NLAASGNRGDLGRAKAVVQTMKQSFPVIETYAVKGPWPSKSRAENLLFFAGEPVEHNT
                     GSDFAAKVAALVERQRLPSEAIALLSTRRMEPWESGAVLTDDYAPYDLLIGMETGEDQ
                     PGGDR"
     gene            51072..51551
                     /locus_tag="VFH05_08715"
     CDS             51072..51551
                     /locus_tag="VFH05_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678338.1"
                     /translation="MGKTMLWVFFGLIMVMAGASPALAHQAGGVEVGDLETGSVVGQP
                     FKELDVDAELYAIEIGNLKTWYPPVTAIDFKARAGRPVVIKVTNSSAAEHGFLLTAEE
                     TQSAPTVLKAQLVLKPGETKYVGIPTSDLFYATTGNILKYRCHLHPAHVGGKLIMFK"
     gene            complement(51618..51953)
                     /locus_tag="VFH05_08720"
     CDS             complement(51618..51953)
                     /locus_tag="VFH05_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678339.1"
                     /translation="MRSRWILLGTLLWSVICTWPSPSQASTGDIGSIVEKFVVRQFPD
                     AASHYWVINQTQWDGDEMIVDVQAIVKERREAEPTLSRFLLLIVAGELKGSQNVPLEP
                     AADCRTEEA"
     gene            complement(52153..52500)
                     /locus_tag="VFH05_08725"
     CDS             complement(52153..52500)
                     /locus_tag="VFH05_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6678340.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAETKGNHVGDGQGV
                     GDNIYVITHDRCTECVGHFDEPQCAAVCPVDNCCISDPAYPETTDVLLEKAKKLNPDK
                     AIDAAKVWSGVRN"
     gene            52702..53193
                     /locus_tag="VFH05_08730"
     CDS             52702..53193
                     /locus_tag="VFH05_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET6678341.1"
                     /translation="MTVTSAQRDQKKTRIIMMILLAVLLISVSMFLVQRDDSKTIIVT
                     FPNGTQIEAEVADTPEKLLFGLAFQEALPSNGGMIYIFESTGHHRVTTKAYRFPVDMI
                     WVDESRHIVSVMENVQPCQEGTCPWHGSTSDDVRYLIQTEAGFIQRQALTVGGELKYT
                     LHM"
     gene            53237..53560
                     /gene="nirD"
                     /locus_tag="VFH05_08735"
     CDS             53237..53560
                     /gene="nirD"
                     /locus_tag="VFH05_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HET6678342.1"
                     /translation="MAIEGFQVVAKVEDLPAGQSKVVTLNNRTIALFNVDGNIYAIHN
                     LCPHEGGPLSEGRVKGFVVSCPWHDLAFDIRNGQGIDGGGYCVGSYEVRVEGGEIHVG
                     ARRKS"
     gene            53583..53909
                     /locus_tag="VFH05_08740"
     CDS             53583..53909
                     /locus_tag="VFH05_08740"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HET6678343.1"
                     /translation="MDATGGLQGDGKRVDATLDQPIHIHLWEDRTRGELWIPSYDASA
                     LALLGDDYVRVAGNNAVDNGQRTFEFKAMKPGTFTILFEKRMGWKFTAEDRRVFHVTV
                     SPNGSA"
     gene            53906..54751
                     /gene="dat"
                     /locus_tag="VFH05_08745"
     CDS             53906..54751
                     /gene="dat"
                     /locus_tag="VFH05_08745"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HET6678344.1"
                     /translation="MTVPETAFLNGRFLPLAEATVSIEDRGFQFGDGVYEVIRTYRGR
                     PFKLDAHLARLDRSAQAIDLRQPYPFERWVEYVTEGLRLGDFPETKIYVQITRGTAPR
                     DHAYAPDLQPTVLMTFRELQPLNATFPSTGVAAMTMKDIRWGRCDIKSLNLLANVLAR
                     QQAKKRGVFESILVRDGQVTEGAVSNVFIVQDGKLITAPEGGWILSGVTRQVVLELAR
                     HEGLMVQERYCSEQELLGATEVFLTGTTVEVLGVVHIDGKQIGKGQPGPVTRSLAKRF
                     LDETR"
     gene            54873..55349
                     /gene="rimP"
                     /locus_tag="VFH05_08750"
     CDS             54873..55349
                     /gene="rimP"
                     /locus_tag="VFH05_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET6678345.1"
                     /translation="MKESRPVVDRISEAVSPILWTLGLELADVVCVGQGSRSVVRVFI
                     DKPEGVTLEDCERAHKALGPALDVADPFPHAYTLEVSSPGLDRPFKRLQDYRRAIGKR
                     VTLKLREPLAGQWRLVGTLTDVADQTVTLTVDDRPTVRAVTLEQRLIAEAKRAVTW"
     gene            55381..56532
                     /gene="nusA"
                     /locus_tag="VFH05_08755"
     CDS             55381..56532
                     /gene="nusA"
                     /locus_tag="VFH05_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET6678346.1"
                     /translation="MNRELISVIDEIGRQKGIDKTKVIGAIEAALQTAAKKRFGQAEN
                     IQVEIDSKTGEISVVSKKIIVESVSNPKAEISLKEARAYDSEAEIGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNYLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKAMLLEVRRTPKDVQVILTRSHPQFVSKLFELEVPEVMEK
                     IVEIKAVVREPGDRTKIAVTSREKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     SDPRVFIAEALNPATIEKVGVDEEKKSALVVVADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKAERDKEIKAALAEEAEAQRQQEEARQQEKAQQEAEG"
     gene            56546..59053
                     /gene="infB"
                     /locus_tag="VFH05_08760"
     CDS             56546..59053
                     /gene="infB"
                     /locus_tag="VFH05_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET6678347.1"
                     /translation="MRVYELAKQLGMENRDLIPELKRLGITVASHSSALEPEAVQKAL
                     EKLASKHKASVKGAGRPGESDAGRQKDADGAHPPAGAKAGVHPTATDDSKPDKRRILI
                     KRKRDEAPSEELTPLGHEPLVAHSTEPPAVTVSPLHSPSESPVVHHEPLVSGTTATTE
                     SPVATKPVPAATPETLTGKKKALEELQAEGLKDKLKKVRKPGRVREEDEVRVRDDAAR
                     WQDLRAIPVQRRDDRSKHVHHSTPAEITKPRKKSVKLTPGMTVKEFAEMIGQRPADIV
                     RKLMDMGQMLTFNQPMHVEAASMIAEEGGFKIEISVEKAGDELLEDIIQVQGEAHPEP
                     RPPVVTIMGHVDHGKTSLLDAIRETKVAEGEAGGITQHIGAYTVTVHGKQVTFLDTPG
                     HEAFTAMRARGAKVTDIVILVVAADDGVMPQTVEAIHHAKAAGVPLIVAMNKIDKPTA
                     NPDRVRNALSEHGLISEAWGGDTIMVEVSAKQKTGLDQLLEMILLQAEVLELKADPHQ
                     LAKGTVVEAKLERGRGPVATVLVQSGTLHVGDVFVVGAFSGKVRALINHTGSKVQQAG
                     PSVPVEVIGLPGVPSAGDVFQAVKDERIAREIAEDRARKQRAAELAGAGKVSLDDLFA
                     KIQEGSVKELSLVIKADVQGSSEALATAIEKLPTEVVKIRVIHNGVGGITESDVLLAA
                     ASNAIIIGFNIRPEPKASDLAEKEHVDVRLYTIIYNALNDIKAAMEGLLEPTLKERVL
                     GRAEVRQVFTVSKAGTIAGSYVIDGTITRASAGVRVIRDNVVVYQGRLGSLRRFKDDV
                     REVQQGYECGIAVENFSDIKSGDVIEAYAIDKIAAKL"
     gene            59200..59487
                     /locus_tag="VFH05_08765"
     CDS             59200..59487
                     /locus_tag="VFH05_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET6678348.1"
                     /translation="MSVVVGLCTIELFLGDSQSLKDKRQILSSLKDRLRQKFNLSVAE
                     IDAQDLWQKGVLALACVANDGRYVNQVLDQAQNMIKSDPIIEIVQSRIELL"
     gene            59541..59945
                     /gene="rbfA"
                     /locus_tag="VFH05_08770"
     CDS             59541..59945
                     /gene="rbfA"
                     /locus_tag="VFH05_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET6678349.1"
                     /translation="MAKTGYSRAERVADQIRMEVADILMRRIKDPRVRSVTVTDVELT
                     NDLRIARVFVTALGTEAETKDVFAGLSKASGFIRSELGRRLTLRYLPEVVFHKDVSGP
                     RGDRVLRLLDEIHDKGHGQSDEHDDAPSSHHG"
     gene            59950..60903
                     /gene="truB"
                     /locus_tag="VFH05_08775"
     CDS             59950..60903
                     /gene="truB"
                     /locus_tag="VFH05_08775"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET6678350.1"
                     /translation="MTATARECDVMDGVLVVHKEAGWTSHDVVAKVRHLLGATKVGHA
                     GTLDPAATGVLPVLIGKGTRIAEYLMPWDKEYRACLRLGETTDTQDATGTVLQRQMTS
                     DVTPDDVEEAIGRFKGEIQQIPPMYSALKVRGVPLYRSARAGKTITREPRTVTIHELE
                     VLTIEGCDVSLRVACSKGTYIRTLCADIGAALGVGGHLRALERTRVGPLTVDRALTMD
                     VVIARHALGRLADEMWSLDEILGALPAFVVDHQTADRVCHGVPVAYSSIVSPRAEEVR
                     RIGRTPVRIHDQGGRLLAVGRAPQTQVDRIAIEKVLVPQSG"
     gene            60929..61198
                     /gene="rpsO"
                     /locus_tag="VFH05_08780"
     CDS             60929..61198
                     /gene="rpsO"
                     /locus_tag="VFH05_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET6678351.1"
                     /translation="MALLKAAKAELIKGYRQHDTDSGSPEVQIAVLTNRISYLTDHFK
                     LHKKDHHSRRGLLQLVGRRRRLLDYLRDIDDARYRAVIERLGIRK"
     gene            61384..63504
                     /gene="pnp"
                     /locus_tag="VFH05_08785"
     CDS             61384..63504
                     /gene="pnp"
                     /locus_tag="VFH05_08785"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET6678352.1"
                     /translation="MAHAVELDLAGRTLRLETGRVAKQADGSIWASYGDTVVLATAVA
                     SQNAKPGVDFLPLTVDYQEKAYAAGKIPGGYFKREGRPSEKEVLTSRLIDRPLRPLFP
                     EGYYFETQVIASVLSSDRTGSSDVIGITAASAALAVSNIPFLGPIAGVKIGRVNGQFI
                     VNPDLEALKGSELDLVVAGTADAVMMVEAGANELPEATMLAAIELAHSEIKKIVAKIN
                     ELRVLSGNKPKRMIATEEIPSDLTGQVKALVAQGIREAIMIPNKTARQERLDLILRDA
                     IEKLKQTDDPNRERHVKIVFHGLEYTEVRNMILEKGSRADGRGPADIRPITSEVGVLP
                     RTHGSALFTRGETQSLAVVTLGTTDDEQRIDALEGEYTRTFMLHYNFPPFSVGEARPL
                     RSPGRREVGHGALAERALKPVIPNKDQFPYTLRIVSDILESNGSSSMATVCGGTLAMM
                     DAGVPIKEPVAGIAMGLIKEDDRVMILSDILGLEDHLGDMDFKVCGTKRGVTALQMDI
                     KIGGITPALMHQALEQAKAGRLHILSCMQKALEAPRTNMSAFAPRIYTMKVKQDKIRE
                     VIGPGGKTIRGIQADCGVKINIEDSGIVTIASVDGASLEKAKEMVNRIVEEVEIGKTY
                     LGTVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKSVADEVAEGDQILVKVLEIDRQG
                     KIRLSRKETMPAPTGAGTKDQTSG"
     gene            63510..64763
                     /locus_tag="VFH05_08790"
     CDS             63510..64763
                     /locus_tag="VFH05_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6678353.1"
                     /translation="MYRKLVLDNGMRVVTERIPTLKSVTVGIWVNTGSRDEQPAQAGY
                     SHFIEHMFFKGTRSRSASEISREIDGLGGEMNAFTTRETTTFYVKVLDQQLQQALELL
                     ADLFHHSRFDSKEVEKEKQVVLEEIRMVQDDPEDLVQELHTGQVLGRHPLGRSILGRE
                     DTIQSLKRPDLLGYIDAHYDPSQIVIAIAGSFEQATVERLVQRYFGKRKPIHGSLRAP
                     RRPPDVRGGVLAKKKQLEQVHLCLGLKGVAAGHRDRYAVYALNSVLGGSMSSRLFQEV
                     REKRGLAYSIYSFLSGYSDVGTITVYAATRPREVERVIDLVCREITRIYKNGIDRKEL
                     ERAKNQMKGSLMLSLESSHSRMSKLAKDELIYGRRTSLEEMLAHIDRVTSEQVSTASQ
                     QLFVLDHLAITALGPLSARALQGYT"
     gene            65114..65626
                     /locus_tag="VFH05_08795"
     CDS             65114..65626
                     /locus_tag="VFH05_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678354.1"
                     /translation="MKRVVLLAAVAVCSVLGLLTAEFAAAADAPGGPSGPTDIVVGGK
                     PLKGEVTKTLKGRVWSQWADNPDGELLFGIQYWNTGEPASGTPAGRSSTDLDVKPPDP
                     LFVCCAWGFVGGTDRNNPYAGWYHAATTVRLAVKDKALMDQIIKASQELVAMEVSLDG
                     RTITGFKVIK"
     gene            65678..66334
                     /locus_tag="VFH05_08800"
     CDS             65678..66334
                     /locus_tag="VFH05_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678355.1"
                     /translation="MKFHMKGWALAAAVALVVSIASTAMAIESFQERFEWGDMSKPTT
                     LQGRVIVLDPYDEAVWINISVFGGNAESGLFWQKVHPGKNVKFYADKGVWEELKKMGR
                     PHAGPAAAKEVPPGSTTDLIEFTAVETEQNHRVIQSVKKLPEIAGAPGKTMSISALRL
                     KDCAGKNEADAGCAAAKQGLNTSPKGEGAVPMQYDVLTPDKKPLGGLIPWTAKYNTGK
                     "
     gene            complement(66389..69025)
                     /gene="mutS"
                     /locus_tag="VFH05_08805"
     CDS             complement(66389..69025)
                     /gene="mutS"
                     /locus_tag="VFH05_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET6678356.1"
                     /translation="MSEEETTPLMRQYREIKRGYPESILLFRVGDFYEMFYEDAQEAS
                     KLLSIALTSRDKSSADPVPLCGVPYHAAQGYIARLLKAGRTVALCEQVEDPKAAKGLV
                     RREVVRLYTPGTLVDTEFLDPAESSFLAAVTRLPRRASGGATRMGLAALDVTTGDFWL
                     MEWEGDEQQVADELARLQPRELLHSESDTVVTQSLSQLHGTRLCARDQAWFDHEEASR
                     VLQSHFGVHSLEGFGCHDTTAGLGAAGAVWRYFRETQPTASPAHVRRLLRRKRGDAMH
                     LDGATIRNLELVRSLGEEPSSGRNTTLFSVLDRTTTAMGSRLLRDWLLRPLTDCHAID
                     TRLDGVDELKERISERVAIRAALRTVQDIARLSTRVTLDVAGPRELLALKHSLGALPA
                     VRQQLECCRSTLLVERRDQWDDCRDLYDLIEQAIDPDAPLSIRDGGIIRSGYHPGVDE
                     LRKAHMEGKGWIAALEVKERERTGIDSLKIRFNHVFGYYIEVTKTNLSKVPSDFIRKQ
                     TLVNAERFMTAGLKELEERVTGAETKLLALEEETFIQVRQRIAQEGVRLQAMAQSLAL
                     IDVLAGLAETAALQRYVRPAIDESGVLRIKNGRHPVVEQLSIDRTFVPNDTVLDQDNN
                     RLVILTGPNMAGKSTYLRQVALIVLLAQIGSFVPAAEAQIGLVDRIFTRVGASDNLAA
                     GRSTFMVEMLETAQILNCASRQSLILLDEIGRGTSTYDGLSIAWAIAEFIHAREHLGA
                     RTLFATHYHEMTQLETLREGIRNYRVAVQERDGDVVFLRKILPGGADRSYGIHVAKLA
                     GLPTPVIARAQEVLEQLEQPDRERECRRVVVESPTCSVLPQPHPLIDEVRQIDLFSLT
                     PLDALNRLAELQKRATESPQRK"
     gene            complement(69018..69365)
                     /locus_tag="VFH05_08810"
     CDS             complement(69018..69365)
                     /locus_tag="VFH05_08810"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET6678357.1"
                     /translation="MIRVILVGTLLLLCLAFFLQNQEQEVTLRYFFGLFEASTPIYKP
                     ILAGFGIGLLVSLILLFPPWVKGRIELRRKTKALQEAEVDLERLRHSVEKLTGRAAPP
                     VPGDLQQREGVDE"
     gene            complement(69362..69940)
                     /gene="tsaE"
                     /locus_tag="VFH05_08815"
     CDS             complement(69362..69940)
                     /gene="tsaE"
                     /locus_tag="VFH05_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET6678358.1"
                     /translation="MHSEPSASNRTPGTSERVWQLALPSPRHTDRLGRAIGSALHGGE
                     SLALYGPLGSGKTALVRGIAAGLGATSGAVSSPTFVLLHEYRGRLPLAHVDLYRLNSS
                     KEVDSIGLDDYFSGSTVVVIEWADKAPGILPPDRLEIQLRHRTVQSRSIRLMATGPLS
                     AALLARTRTKRVSRRLPSGRPRSASARGLSSR"
     gene            complement(69915..71465)
                     /locus_tag="VFH05_08820"
     CDS             complement(69915..71465)
                     /locus_tag="VFH05_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET6678359.1"
                     /translation="MKIVTGIQMQALDRRTILEARVPSLILMERAGIGLADFIQSRFG
                     PLRGKRVTVVCGKGNNGGDGFVAARRLRQYRADVTVLCLSAATQLSRDAAAMFQRWRR
                     IAGPSATKTFRSPEQVESILRTSDVIVDALLGTGLSTAVTGVYGEAIQMINRAGKPVI
                     AVDIPSGLAADTGAVLGQAVRATATVSFGLPKYGLYLGEGIDHAGSIHVVDIGIPAAF
                     VDEIDSRTVLVTKEMVRKALPPRPASSHKGTFGHAGILAGSVGKTGAAALAARAALRV
                     GTGLVTVGVPASVNDVLEAKLLEAMTQPLAETKARTLSRAGVDRILAFMQARTAIAIG
                     PGLSTHPETVELVQSLMKHLDRPSVLDADALNALASRASLLTGCKIPPVLTPHPGEMA
                     RLEPEATSQSVNADRLGTAARFARERGVFLVLKGARTVIARPDGLLAICPTGNPGMAT
                     AGTGDVLTGMLVGFLAQGVPSWEAACVATYLHGKAGDLAAEHLGSAGMIAGDVIEHIP
                     YAFRAISE"
     gene            complement(71525..72238)
                     /locus_tag="VFH05_08825"
     CDS             complement(71525..72238)
                     /locus_tag="VFH05_08825"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET6678360.1"
                     /translation="MARLGVNIDHVATLRQARGGQDPDPLAAAVLVELAGADGVVVHL
                     REDRRHIQDRDLHLLREIIRTKLDMEMAADETMAKIALTLRPDLVTLVPEKRQELTTE
                     GGLDVLTHKERVQQIITMMHDGGIPVSLFVEPDLGQIKTAHKVAADFVELHTGRYANA
                     KRSKEADAEFEAITQAAKLAYKLGIGVNAGHGLNYQNVKRLTHIPEIVEYNIGHSIIA
                     RAVLVGLDQAVREMKALLG"
     gene            72518..73714
                     /gene="alaC"
                     /locus_tag="VFH05_08830"
     CDS             72518..73714
                     /gene="alaC"
                     /locus_tag="VFH05_08830"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HET6678361.1"
                     /translation="MGLSDNFYRLKRLPPYVFAQVQSLKLEARHRGEDIIDFGMGNPD
                     QPTPTHIVDKMIEAARKGKNHRYSASRGITKLRHAIAAWYQRNYDVDLDPETEAIVTI
                     GSKEGLAHLSLAMIGPGDVVLTPTPTYPIHMYSFVIAGGEVRGIELRPDSDFFDDLQR
                     VYRQTFPRPRILVINFPHNPTTAIVDLEFFKKIVAFAKEHHVIVIHDLAYADLVFDGY
                     RAPSFLQVPGAKEVGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKIKSYLDYGIFQ
                     PLQIASVIALNGPQDCVKDTVQRYQKRRDVLVGGLNRIGWNVNKPLATMFVWARIPLP
                     YRHMGSLEFSKLLLREAKVAVSPGIGFGEGGDEYVRFALVENEHRTRQAVRGIRKALK
                     LEGGEA"
     gene            73711..75024
                     /locus_tag="VFH05_08835"
     CDS             73711..75024
                     /locus_tag="VFH05_08835"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET6678362.1"
                     /translation="MISRVGVGIVGFGTVGSGVAKILLENAALIRRRVGVPIELVRIA
                     DLDTTRDRGMALPAGLLVKDAKQILADPTIDIVVELVGGYDFAKRLILEAIDAGKSVV
                     TANKALLALHGEEIFAAASRRGIDIGFEASVGGGIPVIHALKEGLAANSIQSIYGIIN
                     GTSNYILSRMTNEGKGFAETLVEAQKAGYAEADPAFDVAGTDSAHKLAILVNLGFGTP
                     VNFKDLYTEGITHITEYDIAYAREFGYTIKLLGIAKLIDGEVEARVHPTMLGEESPLT
                     KIEGVYNAIQLVGDAVGDVVLSGRGAGDLAAGSAVVSDIIAGARNLLKGAAGRVPAAS
                     FQPNERRPLRMRRMEEIRSLYYLRFMVTDRPGVLAQIAGELGRYGISIASMLQKGRRE
                     GQTVPIVIKTHTATERDVQTALSHINRLSFMSEPTTLIRIEGKDE"
     gene            75028..76086
                     /gene="thrC"
                     /locus_tag="VFH05_08840"
     CDS             75028..76086
                     /gene="thrC"
                     /locus_tag="VFH05_08840"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6678363.1"
                     /translation="MTRWRGIIAEYRKFLPVNDQTPVITLDEGNTPLIQAARLAKKIA
                     PGIDLYLKFEGANPTGSFKDRGMTLAISKAVENGARAVICASTGNTSASAAAYGARAG
                     LAVYVLIPAGKIAMGKLSQAMMHQATVIQVDGNFDQALSIVKTLSAAHHIELVNSLNP
                     FRIEGQKTAAMEVCDQLGDAPAVHVLPVGNAGNITAYWKGYKEYRAANQSRGVPRMMG
                     FQAAGAAPIVLGRIVENPQTVATAIRIGNPASWQAAVDAVKESSGAIDSVTDEEILQA
                     YVAVAGTEGVFCEPASAASVAGVMKLSRQGRLREGETVVCTLTGHGLKDADTAISVSV
                     KPQTVNATTEEVARLLKV"
     gene            76109..77341
                     /gene="apgM"
                     /locus_tag="VFH05_08845"
     CDS             76109..77341
                     /gene="apgM"
                     /locus_tag="VFH05_08845"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET6678364.1"
                     /translation="MKYVILHAEGMSDHPRQELAGKTPLQAASTPHLDRLAEQSELGL
                     LTVALDNGRHGSGLTGTSILGYEPKKYYQGPGPLEAASLGVTVGEHDVVYRCTMVTLK
                     AEGPSGGKAGTPDIKKLGPHIVMDDATAGLITSEEARELIDAVNEQLGSEMIQFFPGA
                     GHRHLMVWVNGKPRAVCVDPQSLVGRPIGDSLPAGDGADILRKLMEASLMILRDHPVN
                     DERREAGHKPANCLWLWGEGRAIPRPSLSERFGVSGVVVSSGDVHRGLGLLSGLEVVD
                     PDRLTNLRTHASVALDELKSKDFAYVHIELPDQVVYDPDVKAKVRAIEEVDTEFVGPF
                     LSGMKTLGPYRLVTVSDPGSVHQGNMAQAHWPYAYQDRTAKPSGSGRRFTEVDAKAMT
                     TAPRDATKFVVRLFAKGS"
     gene            77343..78572
                     /locus_tag="VFH05_08850"
     CDS             77343..78572
                     /locus_tag="VFH05_08850"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET6678365.1"
                     /translation="MALIVQKYGGTSVGSIERIHRVADRVEQAVKEGNKVIVVLSAMS
                     GETDRLMKLAHDVAALPDERELDMLLSTGERVTIALLAMVLRGRGLDARSFTGRQVGI
                     MTDSAHTRARITRVAAERIRDALTRGVIPVVAGFQGINEHSDVTTLGRGGSDLTAVAL
                     AGALKADRCVIFTDVDGVYTADPNIVPSARRINTIAYEEMLEMASLGAKVLQSRSVEF
                     AAKYNVPLEVNSSFKEGKGTLVKREDADMEAVAVSGITGDRNQAKITLVGVPDRPGIA
                     ARLLGPIAKANILVDMIIQNVSHESLTDFSFTIPRVDVKKAMPIIEETAKDIGAKSVS
                     VTEAIAKVSLVGVGMRSHSGVAAKMFDVLSHEGVNIMMISTSEIKISCVIDEKYMELA
                     MRALHAAFGLDQTPVGK"
     gene            78624..>80072
                     /gene="cimA"
                     /locus_tag="VFH05_08855"
     CDS             78624..>80072
                     /gene="cimA"
                     /locus_tag="VFH05_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET6678366.1"
                     /translation="MAKHVPHSVRKSSVPRKPATKAVSAPLEIYDTTLRDGAQAEDVT
                     FSADDKVMVAQRLDDLGVHFIEGGWPGANPKDIEFFRMIKETPLKQATVVAFGSTRKA
                     SNPVQKDANIQALLDAETKVITLFGKSWSFHVTDALGIALAANLEIISDSIAYLRSKH
                     RRVFYDAEHFFDGYKTNPDYALETITRAVAAGAERVILCDTNGGTMPWEIKDICRVVQ
                     SECRVPLGIHAHNDCDMAVANSLVALESGVVQVQGTINGIGERCGNANLCSIIPNLEL
                     KMKRPALGDRLHHLKDVSGFVTEIANLMPNKHQPYVGDSAFAHKGGVHIHAVLKNPAT
                     YEHVVPSLVGNRQRVLVSDNAGRSGLVEKVEAYGIKLDKNDSKAQELMATLKERENEG
                     YQFEGAEGSFELLMRKAMGAHKPSFQLLGFRVIVEKKQDLGHSGSEATVMVKVGDVTE
                     LAAAAGAGPVNALDHALRKALEKFYPQLREVRL"
BASE COUNT        17001 a        22356 c        24063 g        16652 t
ORIGIN      
        1 tcgtcaccga tccgcttcat gagcggtatg tcattcaagg ggaccgcgtc acattcgaga
       61 gtcaacgcac aggagtgaca cgctcacttt cgctcggaga gtatccggct ctgaggagtt
      121 ttgtcgaggc gttccgtatg agtctgatgg gcgaggccgc gcagataaaa caactctatg
      181 aagtcaccct cgatgggacc cctgccaaat ggacgctctt gctgcggccg ctggatcccg
      241 ccggcaagtc tgtcgtcgac tacctcctct tcaccggctc ggacggtcgc atcttcacta
      301 ttgccatccg ctcgccggac ggtgatcgca cggttatgac gctgctttgg agaacaagtc
      361 ggtgaggcag cggtatggaa tcggggccct ctgggttctt gcaatgctac tgggcctctg
      421 gctggttgtc acaagggtgg tggtccacag tgaactgggc gatctcctgc ccgatggaac
      481 aacagcgacg cagcgtctct tgttgacgca gttgcgaagc ggccaggccg gccggatcct
      541 cttgctcgcc attgagggtg gaaaccccga cgagctggcg gacaccagca acgccttgcg
      601 tgagcagctt caggccaccg gccactttat attcatcgcc aacggcaacc aggtggtgga
      661 gcgccgcgac tgggacatcc tgtttcgatg gcgctacctc ctcagtccgc aggtcggtca
      721 tgagtccttc tcgagcgaac ggcttcgtcg cacgctggaa caacggcttc aggatcttcg
      781 gtcgccactt gcgccagtca tcaaaccgac gttacgagaa gatcctaccg gagaggcgct
      841 cgccatactc gcggcagtgt ccccagcgga gacgcctgcc gcacatcgcg gtgcgtggat
      901 gtcccgtgac cggtcaaaag ccttgctggt tgcagagacc cgtgccactg ggttcgatgc
      961 cgatgctcaa gccaaggtcc agcaacaggt gcgcgccttg ttcgaatcgc tggtaggagc
     1021 ctcgccgttc cgattgcgca tgagtggacc cggtgtattt gcggtcgaga tcaagcgcac
     1081 cattgaaagg gaagcctggt ggctgtcgac gactgccgcc acgctcgtgc tggtgtttct
     1141 gtatgccggc tatcgatcgg tcaccctggt gttgttgagt gccattccac tgacttccgg
     1201 aatcctgata ggcatgctgg cggtgcaagg gtggtttggg tttattcatg gcatcacgtt
     1261 gggtttcggg atcgcgctcc tcggcgtggt ggatgattat ccgatccact tgttcagcca
     1321 tctgaccgct cgaggttcgg ctggagaggt cattcgagcg atttggccga cgatgcggct
     1381 gggcgtgctg acgacgacca tcggatttgc ttccctgctg ctggccggtt ttcctgcact
     1441 cacccagctc ggagtgtttg ccgtcgtggg tctcatgacg ggagcggcag tcacccgttg
     1501 gattctccct ttgttcgtcc cagagggttt tgttccgaag tatgcctggg cggggtacgc
     1561 atcgacgctg caacgagcgt cgcgaatgaa gccgctcgtg ccggttgccg ttctccttgc
     1621 cagcgccgcg ttgttgtggt ctcatacgcc tatatgggaa acggatctgg cgcagctcag
     1681 ccctgtcacg gagaggcaaa aacagctcga tcatgaactg cggcaggagc tgagcgcgcc
     1741 ggatgttcgg gatgtgttga tgatcgaggg accagccgaa gaggcggtac ttcaagccgg
     1801 cgaaacggtg atgcccaaga tagaagagtt gcgtcgggat ggggtcatcg caggctatga
     1861 cctgatttca cgctacctcc cgagtcatcg tctgcaacag cagcgacaac agtccttgcc
     1921 ggagccacac gtcctgcgac gaaatgttga gacggcgctc caaggccttc ccttcaggcg
     1981 cggcgtattc actcctttca tcgatgcggt ggaggctgca cgatctcaat cgccggccga
     2041 caggacatta ttcgagggaa ccgcactcgg aatgagaatg gcatctttgc tgttcgaaca
     2101 acaggggcgc tggatagggg tggtgccatt gcgcgcggtt gaggatcgcc aacggttgaa
     2161 gcagatcgtc accgcttgga acgttcccca cgttggctac gtcgatctca aagaagaatc
     2221 caaccgcctg atgaccgcgt accgtgaccg gactttctca gtcgtgctgt ggggaggggt
     2281 ggcgattgcc gtcatcctgg ctatggcatt gaagtcattc aagctggtag ggccggtgtt
     2341 cctgcctatt ctcagcgcgt taatcgtgat ggtggccttg gtcaatgcct cgggcgaatc
     2401 gctgtccgtg tttcatgtcg cgacgttcct gctggtcatg gggctggggc tggattatgc
     2461 cctgttcttc aacaggtcgg agggaagtgc acaggagcga gcgcacaccc tgtatgggtt
     2521 gcttgtctgc gcgaccacca caattctggt ctttggcatt ctggccacct cgacgattcc
     2581 ggtgttgcac gccataggct tgaccgcctc tgtgggatcg ttctgctgcc tgctcttcac
     2641 cgggctgatg gcaaaggaga aggtgcatgc ctccggataa acgatcgatg actccattga
     2701 cgctgtcggc ctacacgctg gtgaccgcga acggccgcgg catcggggca gtatcgcgat
     2761 cgttgcgtga ccggcgcacc ggtttgcggc cgtgtaattt cgaggatgcc ttgctcaaga
     2821 cctatatcgg ccgggtggat ggtctcgagg acttctcgct tggaaacgga ttggcgccgt
     2881 tcgattgccg gaacaaccgg ctggcatggc tcgctctgca acaagacgga ttcatggtgg
     2941 cggtggccga agccaaacaa cggtacggct ccgaccgcat tgccgtcgtc atgggaacca
     3001 gcacgtccgg aattctcgaa acggaacatg cctatgcgca acgggatccc cggaccgaca
     3061 ggcttggaga cgatttcacg gcacgctatc gatacacaca caacatgttt tcgtcaggac
     3121 actttgtccg ggcatgcctg gggctgcatg ggcccgccct ggttatttcg accgcctgct
     3181 cttccagtgc caaagtgttc gcaacggccg cccgatatct gcaggcgggc ctgtgcgatg
     3241 ccgccatcgt tgggggtgtg gacagcctct gtttgaccac gttgtacggc ttttcagctt
     3301 tgcagctatt gtccgcccag ccctgccgcc cttgcgatga agatcgggac gggctttctt
     3361 tgggtgaagc cgccggctat gcattacttg agtcgagtgc gaacgtcgga aggaagggag
     3421 ccgtcgcact gctcggctac ggtgaaagca cggatggcca tcatatgtcc cagccgcatc
     3481 ccgagggctt gggcgccatt agagcgattc agcaatcgct ggaacgggcg ggtcttgtcc
     3541 cctccgacat cgactatgtc aacctccatg ggactgcgac gaaagcgaat gacttggtag
     3601 aggacaaagc ggtccatcac gtcttcggat cagctccagt cgtcagctcg acgaaaggtt
     3661 ggacggggca tacgctggga gcggccggca tcaccgaggc attgatgacg gccatctgcc
     3721 tgaaggaagc atttgttccc ggcacgcttc actgcgagcg tgtcgatccg gcattgaaaa
     3781 gccgcgtcat ccgcaataat gaagcacggc cgatccggtt ggcggtgagt aatatcttcg
     3841 ggttcggcgg caacaattgc agcttggttc tcggagtctt gtgatgaagg tgggaatctt
     3901 gggtgtgggt ctgctgagtg ccggcctcga cggctggatg aagggacgtg cagtgttggc
     3961 cggcaccagt tcctaccgcc ccgggtcggt acctgagccc gagtcaccct tgctgccggc
     4021 caacgaacgt cgccgcagta gcgacgccgt gcgctgggct ctgcatgtcg cagacgaagc
     4081 tgtccgtcag tcaggacttc acgcacagga gctcgccacc gtgtttgcct cttccggcgg
     4141 ggaaatggct gtcttggatc agttgtgtcg cgccttaacc acgcccgacc gggccttatc
     4201 tccgacgctc tttcatcagt cggttcacaa cacggcggcc ggctattggg gcattgcgac
     4261 cggctgtcag caatcctcga ccgcgctggc ctcctatgac gattcgtccg ccctgggact
     4321 attagaagcg gtcgcctatg caacggtcga agaacgtccg gtcctgtttg tggcctatga
     4381 cctgccgtcg cccccaccgc tcgatcgcgc acggccgatc cgatctgcct tcgcggcggc
     4441 gctggtgatg accccatcgg caagcggggc tccggttcat gcagacctca cgctcgcgac
     4501 acacaccaag gccgagatca ccgggatgga ggatcaaacg ttggaagaac tccggtgtgg
     4561 taatcctgcc gctcggttgc tgccgctgct tcgtgcgctt gcacagccag gttcgcatcg
     4621 cgcgagtctg aatgtcctcg acgatcaacg gcttattgtg gaaatcgagt cgtgacaggg
     4681 atgctgaaag ccgagatcgg cctgttgctc cctcacgggg aatctatgtg tttgctggat
     4741 gcggtcgatt catgggacga gaggaccatt acctgccgga cttccagtca ccgcgatcat
     4801 cgaaacccct tgcgtgccgg agggcggttg atgatcaccg cgggcctcga atatgccgcg
     4861 caagccatgg gggtgcatgt cggactcctg aacggtggtc tccgaagcgg gaagcaaatc
     4921 ggctttgtcg gaagcgttcg cgatgtgacc tttaccatca atcggctgga tgacttgacg
     4981 caggacctca cagtgactgc cacccgtctt ctggagggag acagcagtta tatctatcgc
     5041 ttcagtgtga cgcatgacgg atccgcgctc gtcgagggcc gcgcgtcgat ttttatcaga
     5101 gcggcgtcgt catgaagaga cgggccctgg tgaccggcgg cagcgggacg atcggtgccg
     5161 tgatctgtca gcggctggca gcagatggct gcgcagtcgt cgtgcacgcc aaccggcatc
     5221 cgaagtccgc ccagcaggtg gtggaaaaca tcactgctga cgggggggca gcgcgaatga
     5281 cgacgtttga tgtggctgat cccgatgcga cgcgaaaggc gatcgaatcg ctcctcgatg
     5341 aaggtcccat tcaggtgctc gtccacaatg cgggcgtgca cgacgatgcg ccgatggcag
     5401 gcatgacaat cgagcaatgg aaagaagtgc tcgatgtttc gttgaacggg tttttccatg
     5461 tgacgcaacc gctcctcctt ccgatgctag gaaccagatg gggaagaatc gtgtcgatct
     5521 cgtcgctggc gggggtgatg ggcaatcgcg gacaaaccaa ttatgccgcg gcaaaatcgg
     5581 gccttcacgg ggccagtaag tcgcttgcca tcgaactcgc gtcacgtggc attacggtca
     5641 atgtggtcgc tccgggcgtc atcgaatcac ccgccacaag acattcgttt tcacccgagc
     5701 aggtcgcgtc gatcgtcccg atgaaacggg caggcactcc tgaagatgtc gcgtcactgg
     5761 tctcgttctt ggtctcggag caagccgggt acatcaccgg acaagtgatc gggatcaatg
     5821 ggggcatggc gtgaaccagc aggaccattc aaagccagtc gtcctgggat cgacacaagg
     5881 cggcggcatc catggttgcg aacgcgatcg ctgttgggtc gtcattccgg catacaacga
     5941 agccgcgacg gtacgtgagg tggctctgag atcgcgccaa gagtgtgcac aggtcatcgt
     6001 agtggacgac ggctcgacgg acaacaccgc cgagtgcttg gccggtctgg acgtgatcgt
     6061 tctacgaaac gaagacaatg ccggaaaggc agagagtctt tggcgaggat tccagcgggc
     6121 gttggctcat ggagcggtcg ctatcattac gctcgatgcg gatgggcagc acaggccgga
     6181 agagatttcc cggttcgtgg ctgcagcgca ggatgatcct gagacgtttt ttgtcggtgc
     6241 gcggagacgc gagcatcgga agatatcgcc tgggcggtat tgggccaact gcatggcgga
     6301 tttctggatc ggctgggcgg caggcgtgcg gattgaggat acgcaatcgg ggtttcggtc
     6361 gtatccggca cggttgctgc gtgaggtagt ccttcgacat gaccgcgcgc ggagctttgt
     6421 ctatgaaagt gaaatcctga tcgaggcagc aagacgcggg tttccatgcc gaaatattgc
     6481 cgtgtcggcg atggcgcgcc ccggtcagcg ccccagcaat ttccggcccc tcgcggacgc
     6541 gcttcggatc acaaaaatgg tgtcatggaa gctgatgaag cgagggatgt atctgagcgg
     6601 actatggtgc tttctcacgg gcaggtcgaa tgcaacgcaa cccctctccc ggcgggctca
     6661 gctggcccgg tcctcgccaa tcatccagta acgcgtgttg tgccggtcag aagttaactt
     6721 cctgcagcgg ctaaccgcga gcggatggcg ccatccaatc gttgaatcca gctggcataa
     6781 ttttcgtgta tggtttgcgc gttcgcgtca tacttgaggt tcgagctgtc cttgtacaga
     6841 atgctatagg tcttggtggt atagggaata gacacgacgg cttgatggct gcgaatgttg
     6901 agagtgccga taatttcgcc tgatttggca ggtgccattt gccagccggc tgcgactcct
     6961 gccgcaacaa tctcttttcg aacctcgtcc aggctcagat cttttcccga tgcagtttgc
     7021 acgggtgcat ctttcacttg atacacctgc cccccgcctc tgcagccgat catggctacg
     7081 agcgcgacac acgcggctac tgtcgcccat cgtattctct tcatcgctga cctccagttg
     7141 aaagacgacg gctcaggaga acgcgctagc tcttattacg accggatggc tcacgtcaag
     7201 atgggtggcc gcgctgctca tcagggggct cggcacaaag tgtcgggcat agagataatc
     7261 cagcagcgct tcgagcacgc gcaagttttt gggacccatt cccctggtgt tccacttcga
     7321 catgttcttc ggaaacgact ggtctttcgc gtggatgaat tccgacggat ggcagtaaaa
     7381 gaccaggtgc cgggatgccc gctcgatcca ggcgatcatt ttgccgagcg tctgcacccc
     7441 cagcactcgg agggttgcca tattgacagg gaacagaaag gcagacggcg gtatttccgt
     7501 gatgacactg gtacccggtt tgcctaggtc cgcttcagat ggtcgatagg gctgggtcgg
     7561 agcccagaag tacttcagat aatgaacacg tccgaacccc aagtcgaagc gcctggccgg
     7621 gaccgaggaa tcgtagagat agccttcttc ttcaaggatc cgcaaggtgg tttcaccgat
     7681 ccacaggttc ggtgcgcgga aaatgacggg gcgaacaccg gcagcgtgct ccacggcttg
     7741 agtggcttgt cgtatccagg ttcgctgctg ctcgtatccg gcggttcgaa agtcttcgtc
     7801 ctcttcaagg cctccatggg cccatccatg cgtccccaat tgatgtccgc gctcgcgaca
     7861 atcccgaacg agattcggat aggcctcagc aaaacgaccc gtgaaaaaga cggtcgccgt
     7921 catgccataa cggtcgcaac agtcgaggag agcttcgagg cctttctccg aacccggcac
     7981 ccagtcgcaa tcaatggtga agtgcaagtg tttcacgggt gagaaatcgc gtgttcttac
     8041 atcgaatgag tcggagattt ctacgggagt ggctgatgtc cgctgcatga ggctcatgtt
     8101 cgtgttcaac cggtttccgt aaggcggtgc aggtgaggga aaaggcctca gtccgcagtg
     8161 acgagccctt cgatttcgac caacaattcc ttgcggcaga gatcgccttg gagatagagc
     8221 aggtggcttg aagcaaaaaa cggatcgtcc gtgacccggc gtacggcgtc gagatgatct
     8281 cgatttcgca cgtacacttt gaagatcgat tgtaaccgtg cctctcgctc cggggtggcc
     8341 gggacgcaat gggcgcgatc gagcagcgcc cgcaaatttg tcatcgtttc tcgagcttgg
     8401 aggtcggcta aacccttgtg ttggctctga tgcccgacga cgctcgcggt accggaaatg
     8461 aacaactgcg tggcgctgtc cgatcggtac agactggcac gagcgaacga ggggctgcgg
     8521 gggccataga ggcttggata gtcgtacgcg tttacttgac gtgggttgcc taaatgtaag
     8581 gctggatggg cgccggcgag gaaataaatc tgaagggggc cgctttgcgt tcccacagcc
     8641 gttccggccg gcagtgaagc aggaaacccc ggcaatgtgt cggccaaggc ttggtgccgt
     8701 ccgacgcaaa acaaccgata tcgttccagg ccatgctggt cttgattgat agcgggaaaa
     8761 taattccaga tacgccataa atgcgggaac cctgattcga gcagttgatg aaggaggtga
     8821 cggtaggcaa tttcagtggt tcgatcgaga ccacagtccg gatcttccgc cacggtaatg
     8881 gagccgaaca gcaacgagcc gctcacggcg agcgaaaccc cgttctcctt gtacaggcgg
     8941 actggctgat cgcagctcca gagttcgcat cggccggatc ggtcgagcag cggtagatca
     9001 aggtgaacga ccggacaggg cagggaaaac ggaagaggca gaccgaatga gacgacggca
     9061 agcgggtagg tctccgttgt cgtcaactgc cggacaagct catctggctc aaggtaactg
     9121 attttgacgt ggctcgtggg ttccagaacc cgctgcgcgg acgcgggaga ggacgcctga
     9181 tggtgtcgca tgcccatgga tcggatcgac ctatgctgac gccgacttca cagcgtagtc
     9241 ggtcacctcg gttaccgtgg tgcggctgcc acgcttacgc cggcgggcca cgctccagtt
     9301 ttctttccaa ttcaacatga aaacgacgta atagacaact ttaaaaagga aaagagggaa
     9361 gcgcatcttc gattgagcga agacatctcc ggccagtaac gacaggacgg cccgttcaat
     9421 tttcgcggga cgcttcggcg acacgaacag cgcttgaaac gccggctggt tgaatcggtg
     9481 aatgaaccag gaaaaggtgt cgatgcctcg tcgcactatc cgctcaaagt gctttcgacg
     9541 gacggcatat tctggcgagc ctcgaagaaa tgcatcgaca acccctgccc cgagggttcc
     9601 gctgttcagc gcaagatgaa caccgctgga gaacaccgga tcgatgaagg caaaggcgtc
     9661 tccgatcatc aaatatccat ctcccgacat cgtgtcacga cggtaggaga aattggctgc
     9721 agcgtatgcg ggcgtcaaag gcctggctcg ttccatacgc gccgcaatag gcggtgattg
     9781 tcgcaaggtg tccaggagaa attgatcgag cggagtcctc ctggacttga ggtaatcggg
     9841 gtggcacacc acgccgacac tcatcgtccc atctttgaac ggaatcatcc agcaccaacc
     9901 gtcgtcgagc caggccgcag taatgtttcc ttcgtccaat ccggtcagtc tcgtgactcc
     9961 ttcgaaatga ctgaagacgg cggcgctgcc gtgcttcttg ctgcggtgct tgccgctcaa
    10021 ttgtccggac aaaaatgtgt cgcgcccgct ggcatcaaca atgaaacggg cttcccacgt
    10081 ccgagcatgc cccgttcgat cttctgcgta gaccaatgac gtccgtcccg gacggaattc
    10141 ggcccgctgc gctcgtaccc cttcatgaaa ccgcgcacct ttttgcgttg cgttgcgggc
    10201 caggatggca tcgaattcgg cccgtttcac ctcaaaggca tagggttggg agtcatcgta
    10261 tgcctttgca aaatggaaca tctgcgaccg gccaaagcga tgggatacca gttcggcacc
    10321 gtatttgacg atgccgatct tttccacatc aggcaacacc ccgagccgcc gcagcatggg
    10381 aacggattgg ggaagcaaag actccccgat atggaagcga ggatgggagt ccttttccag
    10441 gacatgaacg ttccagccct gttcggcaag caacgccgaa atcgtggagc ctgccggtcc
    10501 acctccgatc accagcacgt cacaacagat gcagggggcg acgatagaag aaaggctgtt
    10561 catctagaaa aagatcgtgg caaacttcgg gagccgtcgc cggagtatac ccaagaaacg
    10621 agaggttggc tagcgggcgt ggaacggaac agaagcttct ttcagcagcc gtgttgaccg
    10681 attctgtcct aagtggtagt ctccgcgccg caattctcac taagagcaac cttggggtca
    10741 ccggagcaag gagggaaata tgagactagt gggcatggtc ctcttcgccg ctctccttat
    10801 cggcagtgcg gcatgtcact cgacacacgt gcaaattccg accgctccga ttgcgccggg
    10861 tgagaagtcg ctgggaccaa catggggtga tcatgtgggt ctgatgctgt tcggattcat
    10921 cccgattcaa caaaacgacc ggttcgtcga ggcctacaat gaggccgttc aaaaagccgg
    10981 ggcgacgcgg ttgacgaacg tgatcatctc cgaacgatgg tggtggggtt atgtgattaa
    11041 cggatatgtc tttaaggtag aaggcacggc tgtcacgaac aagtaatcct acccacatag
    11101 gagaaaccca tgaaagctgc tggtgtacta ctcggaatgt gtctcctctt cacggcgtgc
    11161 gtctcgactc ctttgaaatt cccgtctgca ccgatcggtc cgaatgaaaa atccctggga
    11221 aaaaccgagg ccacgagcac gggaatcatg ctgttcggat tcattcctat caaccaaaat
    11281 acccggtttg aagacgccat gcgggaggcg gctgcaaaag ccggcgggac gagattgacc
    11341 gatgtgacga tctcggagca atggttctat gcagtcgtgc tcgatggatt tatcttcaaa
    11401 gtggagggta cggccgtcgc caacaaatag acgcatcgtg ccgggatgcg gctccggcgt
    11461 cttttcgcct tgaaggcgtc ggagaccgca agacgacaag actcttccct ttccttcctc
    11521 ctaggtactc tggaagtcaa aaacctgtaa tatctcgatg tcatcttggg tattgttccc
    11581 gtgtcgggga cgacgcccag aagttcaaat cgtacggcca tgaacatcct tgaccaattc
    11641 ttcgtttttc atcccgaacg ctggcatgag aaggattggc gctctgccag cgggcttccg
    11701 ctcgaggacg tgtggtttaa ggcggcggac ggcgccacac tctttggatg gtatgttgaa
    11761 gcgcccgcgg accgtcccgt cgtcatttgg tgccatggaa acgctggaaa catcgtcaat
    11821 cggctcgaca acctccggtt cctttttcgc cagaatctct ccgtcttctt gttcgactat
    11881 cgcggatacg ggaaaagcca gaatgtccgg cccagtgagg agggactgta tcaggatgcg
    11941 atcggcgcct acgagtatct ggtccgtggg cggaggctca aacccgaacg actcatcatt
    12001 ttcgggcgtt cacttggtgc ggctgtcgcg gcagacttgg ccagccacaa acccgcagcc
    12061 ggcctcattc tggaatccgc atttccttcg atcgaagcgg ttgccaagca ctattacggc
    12121 ggccttccgg tccattggct cctcggggca caatttcgct taatcgagcg actcccggcg
    12181 ctttcccttc cgaaactgat cattcacggc gatcgggacg atattattcc gcttgaattg
    12241 gggcgccaga tctatgaagc cgcgaagcca cccaagatgt ttcatgtcat caatggcgcc
    12301 gctcataaca atacttatca ggtcggcggc acgcggtact tccagacgtt cagcgacttc
    12361 gctggtcgcg cgatccgctc gtaacacaaa aaatggtttc gcgagcgtgg aacttttggt
    12421 acagtgcgcg accatgcagc gtcaccccgc agcatcccga ccctcagtgc ctttaccatt
    12481 cgtctctggg gcataaatgt gaggatctcg tggccccgcg tgatcctctc ggtgctaagc
    12541 ctgcacgtcc ttctgacggg ctgcgtcgca gtcgcaacaa ctgaacaggc ggaggagagt
    12601 tattcctcga ccgcccataa tgggccggct tcgttattac tggtcgctga cctcggggac
    12661 ggcgccgtgt cggacgtctc agtggcggcc agccaatcgc agggatcgga cggcaccggg
    12721 gaggacgcgt atgacccctt cgcgaaatcc ggtgaggaaa ccgagcagga gtacgatccg
    12781 tgggaatcct tgaacacgaa ggtctttgaa ttcaaccgtc aggtagatcg ctacgtgctg
    12841 aaaccggtgg cgcagggcta tgattttatc atgcccgact ttgtccaagt cggcatcagc
    12901 aacatgttct acaacctccg ttttccgcag aggttcttga ataatgtgtt tcaagggaaa
    12961 gtcaaaggtg ccggcatcga ggtcggacgc ttcttgatca acagcgccgt gggctatgct
    13021 ggatttctag atctagcgaa ggaagtcgat ctcgtcacgc cggaagagga tttcggacaa
    13081 acgctgggtt cgtatggcgt gaagccgggc ccctacctgg tcattccgtt cctgcagccg
    13141 tttaccgccc gagattttac gggatatgtc gtcgatattt ttctcaatcc gatcaactgg
    13201 ctggtggcgc cgattatcga agtcgagggc gtcccgtcgt tgatcgcgca caaaaatcgt
    13261 acgacgacga cgtttgtgca aatcggcgcc cgtgtcggcg agatcgtcaa cgaacggtca
    13321 cgaaacctgg aaaaattcca gggcgttgag gaagcgacgc tcgatctcta caccgccgtc
    13381 cggaacgcat atctgcaatc ccgcgcaaga gcgattcagg aataattcac ggacttaccg
    13441 cttcgatatc acacctaatt cctggcccac cttggcgaag gcctctacgg cgtgctccag
    13501 ttgctcgcgc gtatgggcgg ccgacatctg tacgcggatc ctggcttgac cttccggcac
    13561 gaccggataa ctgaatccca cgacgtacac gtactcctcg agtagccgat ccgccatcga
    13621 ggtggcgagc gacgcctctc ccaacaggac gggaatgatt gggtgatcgc cggggatcag
    13681 ccggaagccc agctccgtca gccgggcgcg gaagaacgcg gcgttttcac tcagccgtgc
    13741 tctcagactg tcgccttccg caaccagggc gagggcctga agggccccgg ccgctatcga
    13801 tggaggcagc gaattcgaga ataaataggg tcgtgaacgc tggcggagga gctcaatgac
    13861 cccggcgcat cccgccgtga atcctccggt cgctccgccc aacgtcttgc ccaaggtact
    13921 cgtcacgatg tcgatacgtt cggcaacgcc gaagtgttcc ggagtccccc ggcctcttcg
    13981 tccgatcacc ccggtcgcat gactatcgtc aaccatcacc gcggcgtcga accgttcggc
    14041 aagttctacg atgtgatcga gtttcgccag atcgccgtcc atggaaaata caccgtccgt
    14101 cgcgaccagc cgcagacgga atggccgcgt ctcgtgcaat ctcgcttcta attccttcat
    14161 gtcgccatga ccatagcgaa atcgtttggc tttgcagagg cggatgccgt caatcacgct
    14221 cgcatgattc agggcgtcgc tgatcacggc gtcgcgttca tcgagcagca cctcgaacag
    14281 tcctccgttc gcatcgaagc aggaaccgta gaggatcgca tcgtcagtcc cgagaaacgt
    14341 gctaatcgcg cgctcgagtt ctttgtgaat gtcctgcgtc ccgcaaatga accgcactga
    14401 tgccatgccg tagccgtgat cgcgcaaacc ctttatggct gcctcgatga ttcgcggatg
    14461 attggccagc cccaggtagt tattcgcgca gaggttcaag acacggcctt gtgcgaccga
    14521 gatatctgtg ttctgcgggc ttaacagctg ccgctctgat ttatagaggc ctttggcgcg
    14581 gatactcgcc aactgctcgc tgacgatctg tttgagggtg gagtcagcca tgcgctcacc
    14641 agcacgtcac ggtcgtaaaa gaggtcagca agtcacggaa aaaggacgac ctttccgcat
    14701 tggcccgtct tgatgagatc gaaccccttg gcaaaatcgc tcatggggaa cgtgtgggtc
    14761 acgatgggct tgatgttcaa ccctgccttg aagagccctg cgagccggta ccaggtccca
    14821 aacagccgtc ggccagtaat cccatgcacg cggatgccct taaagatgat gtcgtttgca
    14881 agatcgcagg tgatcgggcc ggtgggaatg ccaaaaagcg tgacccggcc gccgttttta
    14941 acggcatcga aggccagatg tagcgctgcc gggtccccgg acatttcaag ggaggcgtcg
    15001 acaccctcac cgtcggtgat ctcgtcaatg gctttgacca gggacgcggc cgtctcggtt
    15061 ttggcgttca agacgtaatc ggcgccgacc tgcttggcca aacccaagcg gtactcactg
    15121 atatccgagg cgataatggt ggctgcgccg gacgtccgcg cgacggcggc ggcaaagagg
    15181 ccagtcggac cacaacccgt gatcaagacc gtatgccctg tcagatcctc caccaacgcg
    15241 gcatcgacgg cgttgcccaa cggctcctgg agcgaggcgt actccggcgc aatggcccgc
    15301 gacgtgtgcc aaagcacgtt ttccggcaac accacgaagt ctgcaaagga accatcgcgg
    15361 tctaccccga gaatcttgta attgcggcag acgtgcgcct ggccggtacg gcactgaaaa
    15421 cacaagccac atgtgatgtg cgactctgcg gccacatagt cgccgacctt caccaatttc
    15481 acggcagggc cgacttcgac gacctcaccg cacatttcgt gaccgatgat gcggggcgga
    15541 tggatgcggc tatgagccca gggatcccaa ttgtaaatgt gagcgtccgt tccgcagagc
    15601 gacgtggcct tcacgcggat aactgcttcg tgtgggcctg gcgccggatc gggacagttc
    15661 atcacagtca atccgggctg tggcgcggtt ttcatcagcg cacgcatggc tctattgtat
    15721 cccactggcc tcagcttgcc taacatcagg aaaattacgg gttgcgcggg cggtatggcg
    15781 tcggtaggat gcccgcttat gtgcgcggcg ccgcatcatc gcagggtcat gctccggata
    15841 ggagcgttcg gagttgcggt gttcttgtgc ggcgctcttc tcacggccat ctcaccctcg
    15901 tcagcggcgg atgcgggcag acgctgggcc cgctttgaac tcaaccaaca ggatgccagc
    15961 ggcgatctcc agaccggcaa acccgtggca ttggtgatta ctctgggcgg ggtggcacag
    16021 ggcaccgggc caatcgtggc gttgtgcgaa tccattttgt tcgagccgca actcatcacg
    16081 ctcgagccgg acggcgaatc gtcggcactc aaggcggagg ttaccctcga ccccatgccg
    16141 tcgagccgca cgtcggtgca tcaaaaggcc gcgcgcattc aggtgacgtt cgccagagcc
    16201 cgtcccgaga agctggaacg ttttatgaag cgcatcgtct atgtgacgtt agagcgcgag
    16261 gagacgtcga gtgacacgag tgagcccccc gtcgtgcgtc ccgaggagcc atcgaccggt
    16321 gatttgatcg tcgatgagcc gcaaccgagc ggcatcgctc ctgtctcgac gggagccctg
    16381 ctcgaagaag atttgatgcc gctcggcgat cccggccagg gcaaggccta ttggcagcag
    16441 gtcagcctcc tcgtcagtcg cagttgggcg cgacaggtgc gaggggttcg ccgcagcccc
    16501 agcagcgaaa ccgtccgggt gcgcttcaag ctatatccca acgggcgggc ccaattgatc
    16561 caaattgaaa agggctccgg tgcgcgggaa atcgacgagg cagggatcta tgcggtggtg
    16621 aacgctcagc cgttcccccc cttgcccgag gaggtgggcg atgagccggt agacgtccat
    16681 gtccgcatga gaacgggagc ccgttccacc gctcgcgaag tgcaatctgt cgggaattct
    16741 tcgaatggga aactcgagac accggcgcag ccgaaaaaat agctgttctg caacatagga
    16801 ttgcctgcga tgtgcggctt gcggcaattt tcctcccact cgccgactgc tccgccgccg
    16861 gggcagaacc tgcaggggag tccctcaagc ttgaatcgaa tgtatcaagg ccgaccgcga
    16921 gcgtgcttca acactgctgt tttcgagcaa tggtctggtc tcatcggtca acagggtaca
    16981 ggctgcgcat gaaggcgatg gttctcgata gcccggggga tgtagccggc aatccactcc
    17041 ggctcgacaa tgtgcccatg cccgaacctg gctccagtga agtccttgtc cgcgtctcag
    17101 tctgcgggat ctgccggacg gatctgcacg tcgtcgaggg cgagttggaa cgtcccaagc
    17161 tccccctcat tcccggacat caggcggttg gactgatcac ccgtctcgga tccggcgtca
    17221 atgaacgaac aatcggagaa cgagttggcg tcgcctggtt acagggcacc tgtggccaat
    17281 gtgactattg ccgaagcggc cgggaaaact tgtgcctgag cgcccgattc actgggtatc
    17341 aggtcgacgg cgggtatgcg gaatatgtgg tgcttccggc agcctttgcc tatccgattc
    17401 ctccggcatt ttccgatgaa gaagctgctc cattgctttg cgcaggtatc atcggctatc
    17461 gcgcgttgcg tgtgagtcat attcagcccg gccagcggct tggcctgtat ggatttggtg
    17521 cgtccgcgca cattgccatc caactcgcac ggcattgggg atgtgaggtc tatgtctgtt
    17581 ccctcaagcc gagtcatcag gcgttggctc gggctctggg cgccgcctgg gtcggaggcg
    17641 cctcagaggc tccccccgtt cgcctgcatg cctcaatcat gttcgctcct gccggtgaat
    17701 tggtgattcc tgcgctgcgg gcccttgaac gaggcgggac gctggcgctg gcgggaatcc
    17761 atatgagcac cattccacca ctcgaatatg atcgtgatct cttcggagaa cgggtaatcc
    17821 gaagcgtgac tgccaatacc cgccaggacg gattcgatct cctgcgtgaa gccgcggcga
    17881 ttccaatcag gccccgcacg caacgatttt ctctcgatca ggcgaatcac gcgttgcagg
    17941 cgctcaagtc cggcgccgtt aacggcgccg gcgtgttggc catccattag gaacgagcct
    18001 acacgcatgg gtggtcgcta acggtatgtt aggatgggat gatgcgacgc tcggtttggt
    18061 ttgtccttgg cttaactttg gtgggatggg gcacgcctgc cgcttccgcg ctggagttta
    18121 ctgccgatca aatcaccaag attgacggtc ggacccacaa agcgtttatt tattatcgag
    18181 accagatgtg gcgaatcgaa catcattcgc tcgggccggt caacgtcacg atcgtccgca
    18241 aggataagca tctggtctgg ctcctgttat cccggatgaa acatttcaag acactgccgt
    18301 acgatgtcga ccaggatctg cgggtcaccg ctcgactcga tggagagatc tcgcggcagg
    18361 aaatcgggac cgagatccgc gaaggacatc ccacgaccct gtatgaagtc acgaccaagc
    18421 agggcgagca cgtcgaacac tattaccaat gggtcgccac cgacctccaa tttcccatga
    18481 agctggcgaa gaaggacggg agttggattg tcgagtatca gcatgtcaaa atgcgctccc
    18541 tgtccgatta tctgtttaat ctgccggtga attttcaacc acttgaggag ttcgacgcct
    18601 ctccaccggt gtcgaaggaa caatgaccaa tcaaggaggg cgtctcgtgc ggagagcgaa
    18661 actgataggg gtgatggtcg cggccatgct catgggaacg agcggatggg tgtcggcctt
    18721 ggacgttgcg gatgtcacaa gagagtggac agctgatgga aaaaagattg cggctgagcg
    18781 ggcgaagcta ccggctcacg acgagatggt gaaaatcccc gccggcacct tcctcatggg
    18841 cagcgacaag aaagtcgatc ggaacgcgta tcaacccgaa ttcccgcagc gaagagtcta
    18901 tctggatgcc tacgaaatcg acaaatatga agtcaccacc gtgcaatttt tgaagtttgt
    18961 ccttgccacc gataggaagc cgctcatcga ctggcaatac gagggaggaa actttcaaga
    19021 gaccatggcc aatcatcccg tgatgcacgt gtcatggttt gacgcggatg cctattgccg
    19081 atgggcgggc aagcgcctcc cgacgtcggc agaatgggaa aaagcggcac gcggtgagga
    19141 tgggcgtatt tatccctggg gcaatgagcc ggcaggattg agccgtgcga acttcggacg
    19201 taccggattg tcggggccgg tgcgggaccg tcccgagcgc ctcttgttgt atccgccgat
    19261 tatttcggtc gataagtatg acaatggggt cagtccctac ggtgtctttc aattagccgg
    19321 gaacgtggcc gaatggactg ccgattggta cgacccgcat tattacaaaa cggcgccaga
    19381 tcggaatccc aagggccccg aaaaggggac gcaacgagcc ttccgtggcg gcggctggat
    19441 tgatagcacc ccttcggtca ggcctgcgca gcgaaacggg acggatccca acacgaaaat
    19501 gaactggctg ggttttcgct gtgcacggga tgcgaaggat gccggcgacg catcggacgg
    19561 gaaaccggcc cagacaagtc ttcaataaac ggtggcggcc tcagtccggt tgtctgggca
    19621 acttggcttg ccggagcgcg tgaatccgct cgcttaactc tttgaccagc acgcccatct
    19681 tgggatgtga cggctcgaaa tcgacgggaa ggtcatagtg tttcagacct tcgctggcgg
    19741 ttggtccaat cgaagccacg actattttct tgcacgattc cctgaacaac tccgtcttct
    19801 ggtcttgctc ggcgagttgc atgacgtgat caacctgggc cgcattcgtg atgaccatcg
    19861 cgtcgatcct gccgttgacc acttccgtga tcgcggaatg taagggagca gtgtcttccg
    19921 gcaacgccca ccgatacacc ggcaccgaga agacgtctgc gccgcgcttt tgtaagtctt
    19981 ggatcatgtc cggctttgag acgccgtatt cctgaatagc gattttgagg ccttgcactg
    20041 gtcgataata atccagggtg gcgatgacat cgtgccaggt gttgggttcc ggaacggtga
    20101 tattggcctc cacccccagg gtcttgagaa ttgcgagcgg ttttggaccc cgcgtcacga
    20161 tgatcgtttt ttttagggca tcaatgatct ccgccagtgg ataacgcgtc tgaaggatct
    20221 ccaagagagc tttcgtgccc acgcccgtca tcagaatcag gatgtcgagt tgattgagaa
    20281 tgagcttcac accaaaacga aacaccgcag agttgtcttg aagaggaatg gggatctcgc
    20341 gaagggctga gacgaccaga gggtggccgt tgtggcgttg gatcaaccgg gccatctcca
    20401 cgcccatccg gctttcgaag gagagcacgc gcaaaccatg aaaatctgtg gggatcaccg
    20461 gaggagatgg agttggtggc tcggccgccg attctacggg ttccgtacga gcctgcgctg
    20521 aattgtcagg ttcaccgcaa atcgacagga gtgccacgat gtctgacgcc tcatccagca
    20581 acgcttgaat cgactggtcc tggtccgtgc cggcggccgc gaggagttcc agccaaaaaa
    20641 cgcagtcatc ggcgtcgccg gaggcgaggt tgcggttgcg atgaaaatcg tcttcggacc
    20701 aggcccgcgc cagtgcgcgg cagttggccg cgagtgtcgt agcggctttg acgagcggac
    20761 tttgaatggc gagggtttca ggcgtcttgg gaagggaacc acacagtttg aggatgccga
    20821 tcgcgaactg cttggtcttg ttccggagct gatctgtttg ttccgatccg gtcatgacac
    20881 gtttctatca cggaggccgc ggggatgctc agcgaaacct tattttgcat cgcgagcaca
    20941 gcggaaaccg gtcgattcgt tcctgacggt ggggtcgctg tccacccgtg tgaaaatccg
    21001 gaccgtcgga gtctcgttct gccagcccga tccgcgcaat acgcgcttcg taccggtctc
    21061 gggccctttc ggattgttct tgggactttt ctcgtaatac cggggatcgt accaatcctg
    21121 gacccactcc cacacattgc cggccatatc atagagacca tacggactct ttccagcctc
    21181 gtaactgccg accggcatca aggtcttttc gccgatccaa cgctgattgt aattcaaatg
    21241 tttcgttgtg ggctcgatat tgccccaggg gaaacgccaa tcatggttgc ctttggcagc
    21301 cttttcccat tctgcttccg tgggcagccg cttgccggtc cacctgcagt aggcgtccgc
    21361 atccgtccag tccacgttga ccaccggacg gtctgcgatc gactccgtga tccggtcgcc
    21421 ctcccacagg tttctcgtgg gattgatagg attctgcgga acgcgatggc ctgtggcttt
    21481 cacgaattcc agataacgac cgttggtgac ttcatacgtg tcaatcatgt acgcgtcgag
    21541 gtacacctca tggcgcggat attcgtcgcg tccgccgtcc cggtcaccaa gcgggacacc
    21601 catggggaac ggccctgcag ggatcaggac cattggagcc gaggctcctt ccccgagctg
    21661 tgaattttcc gcacggagat cgcaaggagc aagcaggatc actccaatga tgacaaacgg
    21721 caagagcgaa ggcataaggc actcctggtt gtgacgaaga ggtatcgtaa cacattggca
    21781 aagcgactcg cagggcttca ctcagcgaaa ttcaaattga cggcgatccg gaagcccacc
    21841 tactatacgg ctgcggagga tctcgtgtca tgaggcgagc tgtccacaag ggtctccggc
    21901 atgtggcatt gcgcgtgacg aatctggcac gctcgcgggc cttttatgaa gcactcctcg
    21961 gcatgcaggt tgtgtgggag ccggacgccg acaacgtgta tcttagctcc gggacggata
    22021 acctggcgct gcatcaaata tcggcggcgg agttggacgc gtatcggcct ccgcaggcgc
    22081 agttgttgga tcacatgggc gtcatcctgg aggatcggga ggcggtggac gggatgtacc
    22141 gtgagattga gacgaaagtt gaatcgatgg gagggaggat cgcgaaagcg ccgaagcaac
    22201 accgggacgg cagctactcg ttctattttt cggatcccga cggaaacctg attcaagcgc
    22261 tctatgagcc gacaatcagt cagacatgat catgaatacg atttgggaca gcgtatcgcc
    22321 tgaccactat ctcagtctgg ccccctggtg ttgggtgcaa ctcgagagtg ccgatccccc
    22381 cggtcctttt ccgtttgtcg ccggccttgc tccggaagtc gttggcgcac tgcacgaagc
    22441 gcatagctta ctcgcgagct cgatcgatac ggccatcagc gacgtattcg caaagcgggc
    22501 tccgctcgac gacccacttc gttccaggcg gctcgaagac gcgtatgccg aattagtgaa
    22561 tgcccgcccg tacctgagcc ggcacatcaa atgcggtcgc acggttgatg gacgcttcca
    22621 gtgggacatc ccattcgatc ctaccaagtc ggccaccgtg acgaatggcg gtctccgaat
    22681 ttttcatgca gtcaagcggc aagccattcc gatcggcttc gatcagagat ccctggggcc
    22741 gatcgtcggg aggttgcttg ggatgctgga cggtacgcat cgaatggagg agatcaagac
    22801 ggtggtgcgt tcctcaccgc gggaggccca gcaggtgctt tcgcgcttgg tcgactcgtt
    22861 gcatcaatat gaatgtttgc ggagctcgtc cgaagcttcg ataggccggc gttggtttga
    22921 tgtgatccag gatcgtgata cggttcacct cgggcatgcg gccctcttat accgacaacg
    22981 ggacgacgcg tttctctttg atccctggct cctcccgtgg tttgcggaat cgtccgtccc
    23041 gtcgctctgg acctccctgc tcccaaaacc cgcggcaatc tttcttacgc acgaccacga
    23101 cgatcacgtc gatccgcgaa cattgctgca ccttcctaaa gacacgccag tggtcgtacc
    23161 aagccgcaag aaccgtcatc gcttgtacta tgattacccg tcgctcctgc gcgagttggg
    23221 atttacacga gtcgtcgagc tcgcgcatgg agaagcctgg cctttcgagg gcgggaccgt
    23281 cctttctgtg ccgttctatg gagaagatcc ctgcgatctc caaatgccgc ggaactgtta
    23341 cctcatcagc gatcgaggcc ataacgtgct ggtccacgcg gacagcggac cgacaaacga
    23401 tggacggtcc gctttaaagg atcacgtcat cgctcgcctg gtggaaaagt atgggcccat
    23461 cgatctggtg ttcgcttccc aacagcagct gcttgaaatt cgcagccaag ccgctcacgc
    23521 cgcactctct catccgggaa aatggctgga cgtgggagaa aacggctatt tgaccaatga
    23581 ctatctggcg gatctctgcg ccacggcgaa ggcgagattg ttcgtctcgt acgcgaccgg
    23641 cggtgcggac tggtatcccg atcatctctc cttcatgttt agtacacgca accctgctcg
    23701 aactgcgctg ctgaccgccc attgggaacc tcctgaaaac ttgaaagcgc tggtgacgcc
    23761 ccacggctgc gggtatcatt acgcacgggc gttggatgta ttccgggaga ccggcggacg
    23821 gatcgaagtg cagccagccg gagaggacct catgccgtta catctctatc gcgccgttca
    23881 tggcgaaccg cccttcatga aacaaggcgc gcgcccgtaa cagacgacaa catcgacaag
    23941 gaatgactcc atgagatctc tcacgctcgt gacaggagtg gccggattca tcggttttca
    24001 tgtggcccgg cggttaatcg atcgcggcga tcacatcatc gggctcgaca atgtgaacga
    24061 ctactatgat gtgcgtctga aagaagcccg tctcgcgcaa ctcacggcgt tgaatgggtt
    24121 tcagttcgta aaattggact tggcaaaccg ccagggcatg aaggagtttt tctcggaata
    24181 tccgattcga cgcgtcgtgc acctggccgc tcaggccggc gttcggtatt cgctcatcaa
    24241 tccgcacgcg tacaccgaca gcaacattga aggattcatg aacatcctcg aaggctgccg
    24301 tcaactgaaa acagagcacc tggtctatgc ctcgtccagt tccgtctatg gcggcaacac
    24361 ccatatgccg ttttcaattc atgacaacgt tgaccacccc atttctctgt acgcagccag
    24421 taagaaagcc aacgagttaa tggcgcattg ctatgcccac ttgtacgaga ttccgtgcac
    24481 tggcctgaga ttcttcaccg tctatggacc gtggggacgg ccggacatgg cgctcttcct
    24541 cttcacgaaa gcgattctgg agggtcggcc gatcgaggtg ttcaaccatg gaaaaatgca
    24601 acgcgacttc acctatatcg acgacatcgt ggaaggggtc attcgaacgc tcgaccatcc
    24661 ggctcaacca aatccgtctt ggtccagcga caggccggac gcggggacga gcctcgcgcc
    24721 ggcgcggatt tacaatatcg gcaaccatca gcccgttgaa ctcctgcact ttatcgaggt
    24781 actggaaggc gcgttgggca agaaggcgga tatgacgctg ctgcctatgc agccgggaga
    24841 cgtgccggcc acgtatgcgg acatcgacga tctcgcccat gatgtggggt tttcgccatc
    24901 gacatcgatc gaggtcggca tccctcgttt tgtagagtgg tatcgatcgt tctacaaggt
    24961 gtagagggac cgaggcacct cgaacatgac gtggcagcga ggcttataga cagccgattg
    25021 gcacgaagcc ggcaccgaac atcttcgcca gggagagata gcgagcgttg tcatagaacg
    25081 agaagctcgc accacgctgg ttctgtcccg acagatcgcc gccgtaggaa gggtcagacc
    25141 caaaataaaa cccgccgcgg gatttctggt tcaagttcag ccactctcca tacagggcgc
    25201 agacctccgc ttggacgaca ccggttgatg cgacggtcgc atgccagtct ctggcccaat
    25261 cgggcacgtg ccgctgatca cctgtgacgg ctctccggcc gcggtctcca tatgccgcca
    25321 tatcctcatg agtagtggga gcgggccgat atgtcggcat ctggtccagt gacggcacga
    25381 ccgacagctc cttcagcgtc atcaacgtac aaccggtccg ttccttattc gtgtagatgg
    25441 atgagccatc agcctgcgtg cattcccaca tccgtgccgg atcgaaggac ggcgagtcgg
    25501 gagaagcggc caatgtctga gacgcaagca tggcgagcat ggttccaaga acgagtgaga
    25561 accaggttga tagccgcata atgttacctc cgctgaagct gtggaacagg cgcggtgtgc
    25621 gcttctgctc cacaagctat gccaccgcag aggaatgcgt ctattcatct ttggaggggg
    25681 tcgaaaaggc tgccggttgg catctggggc tggcgagcgc caacgcatcg atggctgtct
    25741 ggggcgtcat gtcgtggagg cctcggaaca ccaattcggc ttcatttagc atttcaaggg
    25801 gataggttgt gctcaccgcc aacacgcgca tgccggctgc tcttgccgat cggatgcccg
    25861 caagcgaatc ttcgatgacc agacactgag aaggggtgat cctctcggcg gaaccgcgat
    25921 tggcgttgag ccgatcgcag gcgaggagat agatcgccgg gtctggcttt ccgatcggac
    25981 aatcctcggc cgtgaccatc acctcaaatg cctgttcgat cgcactaccg gccaacgcgt
    26041 cctcgatctg cgcccgacgt ccgcccgagg cgatcgccaa tcggtagcgc ccttgtgccg
    26101 caaggacaaa atccgctgcg cccgcgaaca gcggtggtct gtgatgggca ctgtgcgccc
    26161 gaaagaggtc cgctttccgg tcggtgatac ggaggatccg ggcttcatcg cacatgccgt
    26221 cgcgctgttc gagcagcagc cttgtgcagg tgcgctcgtc cattcccaaa tacgctccat
    26281 agtagtcttg tgtggtcaat gtcaggccgg cctcggccag tgcctgctga aagcaaagga
    26341 ggtgaggcgt ctcgtcatcg acgatcacac cgttgaagtc gaacaccatg gcgccaatca
    26401 cgccggcact ctacgcgagc cttccaaccg gcacaagtgg caagaccttg ttcgtgtcgt
    26461 tgcactgggg tattctcccc cgacaggaag cccaaccacc tgaccgtctc attcatcaag
    26521 cggcgtgact aggcacgcca ttcgccagac ggcttttcta tcctgacgcc actgctgcta
    26581 tgactgatta cggcgtgctc ggcaatctct tggtcatttt ttccgtctcg attgccgttg
    26641 tcttcatgtt ccatcagttc cgactgccgt ccatcgccgg tttcctggtc gccggcgcgc
    26701 tcatcggccc tcacggcttg aacctcatcg ctgacatcgg cacggttcaa gtgctcgccg
    26761 aaatcggcgt cgtccttctc ttgttcacga tcggcattga attttctctc gtccagctcg
    26821 cgtcactgcg ccggctgctg ttcatggccg gacccattca ggtgggcacc gtgctggcgg
    26881 tcgcgtgggt ggccgccatg tcggtgggca tgacgtggca acaaggaatt ttttggggat
    26941 ttcttctctc gctcagcagc accgcgatcg tgctgaaaac gttggccgag aggggggaca
    27001 gcgattcgat tcacggtcgc acgaccatcg ggatcctgat ttttcaggat ctcgcagtcg
    27061 tgccgatgat gctgctcatg cccattcttg ccactcgagc cgaggggtcg ctgacgacga
    27121 tcctgttcac gctggctgag tcggttttcg tggtgggatt gatcattgcc gcagcctggt
    27181 acgccgttcc gaagctactc gagcacatcg tgcgcagcag aagccgcgag ctgtttttgc
    27241 tgacggtcat cgtgctctgt ctgggcatcg cctggttgac ctcactcgga gggttgtctc
    27301 tcgcgctcgg cgcgtttatt gccggactcg tgatctcgga atcggaatat agtcaccagg
    27361 ccatggccga tgtcctgccg tttcgcgaca gcttcaacag cctgtttttt gtgtccatcg
    27421 gcattctgat ggactggcgc gtgttgatcg accacccggt tctggtgttt ggcctgttgc
    27481 tggcgatcct cttcgtgaag tttctggccg gggcagcggc gtctttcgtc ctgcagattc
    27541 ccccccggtc ggcgatcatg gtcggcgtcg cgttggcaca agtcggagaa ttcagtttcc
    27601 tgctggccca gcaggcgcag gagagcggct tgctgcgcgg tgacccgtac caagtgtttc
    27661 tttcggtgtc cgttctgtcg atgatcgtga cgccgttttt aatgcaatgg tcgccggcaa
    27721 tggcccgccg ggcggaagcc attcagcggc tccggcgatg gctaccgaca agaaccgaag
    27781 cgcatgtcct ccaaacgcaa ggaagccaca tccgcctcaa ggaccatgtg atcattgtcg
    27841 gctacggctt gaacggccgg aatctggcgc gcgttctgag cgaaacggaa attcctcacc
    27901 tggcgctcga cctggacggt gacacggtcc gtcgagagtc ccgccacgga gtgcctatct
    27961 attatggtga tgcgacgaat cccaacgtct tacggcatgt caagatcgaa gaagccaagg
    28021 tattggttgt ggccatctcg gatccgttca ttacgaggcg gacggtccag gtagccaagg
    28081 tgctcaatcc gaagatccgc gtggtggtca ggacacgcta cctgcgcgag ttggaagagc
    28141 tgcacgatct cggcgccgac gatgtggtgc ccgaagagtt tgaaacttcg attgaaatct
    28201 tcgctttggt gcttcgaacc tacaatcttc cccaggactt catcaccatg aaggctgaac
    28261 aggttcggcg tgaagggtat gcgctgctgc ggcgcagcga gctgccggaa ctggcgcacc
    28321 acttacgcgg cggaacgttg accgacgcag aggttgagac ttgtcgaatc gacgatgagt
    28381 ctcctgccgt aggaaagacg tttgccgatc tctccattcg accgcgcacc ggagcgtccg
    28441 tcatcgcctg gactcgcaat ggcatcacgg aatcgaaccc ctccttaaag acgaaactca
    28501 aaagcggcga cattctcgtg ctcctcggct cccgcccgca gatccggcga gccatgagtc
    28561 ttctgttgtc cggagagtag tccatgactc aggactcgag gccccggcag attgcatcac
    28621 gatcgttgta ttgacatgaa aaagatgtgg agactatagt caaaaaccgt ggaaactcaa
    28681 tgaatttgat tctggagagg gacgtcatga caactaccac cccccaaatg gatccggcag
    28741 tggcacttgc cgatctccaa gcctcaaaac cggataaggt cacgatcgtg ctgctgagcg
    28801 gtgacttgga ccgtgcgatg gccgccttta ttattgcgac gggtgcggcc gccatgggca
    28861 tgcaggtgac gatgttcttc accttttggg gattgaacac cattcggcgc aaaggcgcct
    28921 cgagttcggc caaagattgg cttcgccgga tgttcggggt attgaacaag ggcggggctg
    28981 agagcttacc gctttcccgg ttccatttcg gcggtattgg gaccagcatg atgcacaaag
    29041 tcatgaaaca aaacagaatg cccggtgtcc ctgagttgat gcaaaccgca atagatttgg
    29101 gcgttcgctt cattgcctgt acgaccacga tgggcctgat gggtatctca aaggatactc
    29161 tcatcgacgg catcgatcag tttgccgggg tcacaacgta tctggcagag gccaagcaag
    29221 ggaatgtgaa tctgttcata tagctcttcc cgaggactat tgaccaacaa ccgggacagt
    29281 cctggttccc tgaggagttc caatgaaagc agacgtaaaa ctcgatacac tcgggtactt
    29341 ttgtccaatg ccgatcatca tgacgtccaa gaaaatcaag gaactcacct tggggcaggt
    29401 gctcgaagtc gtctcggacg acgaggggat caagaaggat atgccggctt ggtgcgacac
    29461 gactggtcat catctcatgg ggctcgaaga ggagcaggcc acctcgggac cgatatacaa
    29521 agcgttcgtc aagaaaacca agtaatacgc gcagtggttg ggcgatgtga gcggaagccg
    29581 gaggatgttc atcggacctc cggctttttt attgcggagt gacggcacgc gatggatagg
    29641 atcgtagagt gtgtgccgaa tttcagtgaa ggccgcagcc aagcgactgt tcaagcgctc
    29701 atcgatgcca ttgcgtcagt tccgggcgtg agactgttga atcacacctc ggatcgtgat
    29761 catcatcgct cagtcctgac tgtcgtgggg gacctggaag agatggtgga agcgatgttt
    29821 cgatcggttc gcgtggcgac cgatctgatc gatctccgca ggcattcggg cgtgcaccca
    29881 agagtaggcg ctaccgatgt cgttccgttc gtccccatca aaggcctctc aatgcgagat
    29941 tgcgtgcagg cggccaaacg tcttggccac cgcgtcggaa cggaacttga gattccggtc
    30001 tttctgtatg agcgtgcggc gtcgcaccgt gaccatgctt cccttgaatc ggttcgtcgc
    30061 ggcggcttgg atggcctcgc gttccgcatg gccaccgatc cggactggtc gccggacttc
    30121 ggtcccagtc agcttcatga gacagcgggt gccatcgtca tcggagccag accgcccctg
    30181 gttgcgttca atgtcaacct gttgtcgagc gatgtgaatg tggcccggtc catcgctggg
    30241 aagattcggc agtcgaacgg agggatcccg catctcaagg ctatcggtgt agagctggca
    30301 agccgtggca tagtgcaggt ggcgatgaac ctcacagact acgaagcgat accgcttcat
    30361 gccgcgttcg ccgcagttca gtcacaggcc gccgagcgag gcgtatccgt tgccggcacg
    30421 gaaatcgtag ggctggttcc acttgcagct ctcgttgaga cggccaagga ggctctcagg
    30481 ctcgatcact tcgaaccgtc gcagcttctt gaagcacaaa tcgaggccgc gtggtctcgt
    30541 ggagaaaagg ccaaaggcag ccggaccacc gtggcccaac cctttcgctt gcccgtgccg
    30601 cgattcatcg aagcggtggc tgcgcccacg gccgttccgg gcggaggtgc cgttgcagcg
    30661 ctggtcggtg ccctggcgtc ggcgctgggg atgatggggg ccgggctgag tcggcagcca
    30721 gccacagaac gtcgtctgaa ggacatcgtc gagcggttga gccatctgct gcaggccgac
    30781 ggtgaggcct accgtacctt tgtcgaagcg accagacggc cgaaaacgga tgagcagcgc
    30841 tcgatggccg tgtcatccgc tcttcacgtt gcaacggaaa taccccttga aattgcggaa
    30901 cagtcagcca aagccggtac gctgctccat gcctgttcat gtggggtgaa accccgcctc
    30961 caatcggaca tgcaggtggg tctgattctg gcgattgccg cggccgaggc cggtctacat
    31021 acggccggtg agaatctaaa aatacagccg aatcataagc ttaggggatc tctcgctgcc
    31081 agagtgcagc ggctgtcgca aaatcttgag gaactgaagg tgctatgcta cactccgccc
    31141 cctggtcaat cgggcaaaca ttcagcgcag gccttgcctg ggaaagtgcg gacgcgagac
    31201 gaatggaaat taaagtcttc aataacaacg tcgagaaagc gctcaaagtt gcgaagaaaa
    31261 agcttgccgg agaaggactc tttcgagaac tgaaacgtcg gcggtattac gaaaagccga
    31321 gtgtccgcaa gaaagctaaa cagcgggaag cacagcgccg ccgtcaaaag tggctgtcca
    31381 agcgccgccc cgaatagtct tccgtctcat cagtcgccat ccgctaccac cagtcttgtt
    31441 tcgcttctct catgcgtcag gaccagatcc atgaggcgat ccggatcctc aaacgggagg
    31501 tccggcagtg gcaggaaccg gtggttggtg tgattgccag aacctctgct cgggatccct
    31561 tccgcattct catttcctgt ttgttgagcc tgagaacaaa agacaagacg acgagtgaag
    31621 ccagcgcccg gctcttcgct ctggcataca ctcccgaggg actcctcaac ctacctcgga
    31681 gaaccattga gcgcgtcatt tatccggtgg ggttttatcg aacgaaggca aaagccattc
    31741 atgcaatttg ccgccggctc ttggaggtgt acgggggcac ggtacctgat tcgatcgatg
    31801 agcttgtcac tctgccaggg gtcggccgta agacggccaa tctggtggtc acggtgggct
    31861 acaacaagcc gggtatctgc gtggacatcc acgttcaccg catcagcaac cggtggggat
    31921 atgtgaagac cggaaagccc gaagagactg agcaggcgtt acgccataag ctgcccaagc
    31981 ggcattggat tatcttcaac gacttgctgg tgccctatgg gcaaaacatc tgccagccgg
    32041 tgtctccctt ctgtagccga tgcaaactgg ccgaattctg cgatcgcgtt ggcgtggtca
    32101 ggtcgaggta ggactcgagc aatcggctcg ctccgggcga tcaaataaac agcttcgttt
    32161 ctgcgtctgc cgtcagggag agaatcgcag gcaagcccat cacttcgtcg acctgctttg
    32221 agacggcctc gccgtccact cccatgtact ccagaccggc actgcaggcg atgatgcgaa
    32281 ccttccccac cagcttggcc tcttcgaaca tttgcgaaat cgtcggcacg tgcttctctt
    32341 tgagcaaacg ccccacgatt tccgccgaac cggaatattc cggaggaaag tcgatctgat
    32401 ctaacttgcc ttccgccagc tttttgatag tccagaacag caggatgacg atcacgtcct
    32461 tccccatggc ggctgcagtc aacccgagcg tcgccacctg atgaagctta tcgtaggtgg
    32521 cattatgggc aaagagaacg aacttgggtg aaggcgggct catgctcacg gtttgggaac
    32581 cctggctttg acgctgttga tataatcatt gattgccgcg tcaacctccg taggcgccag
    32641 cggccggtca tgtgtttcac tctcatcaag cagataggtg tcccgctctt tttcctggcg
    32701 gacgatcacc gtattctcag gtgtgacctc caccaacatg aaccattcgc gaaggccctg
    32761 ggtgatgacc acttccttac ccaaaccctt cttgttgatg gcaatgacga gatcatcgga
    32821 ccccgcagac aggactgggg aggtgcatac ggctaaccaa aggagtacgc aacatagtcg
    32881 cgtggtgaac atggtcgcgc gcatggcgcg attatagctg cggccagaac ctcagtaaac
    32941 cgcccaggcg tcgggctgat gatctacgtc ccataggtcg catgttcgcc aggtcgcgtg
    33001 ttacgatgcc gccccgatgg attggcttct ttcgtcagtt cagcactatg tgtctactga
    33061 gaccttgatt tggctcactg tcctgtcaat catctttttc gtcgggacat tgattgcgat
    33121 ccccttcatt ctggtgcgct tgcccgcaga ctatttcgac gtgcgggtcc cgcgtccctg
    33181 gatgcaggat caccatccgc tgcttcgggt tgtcggccac atagtcaaga atgcgctggg
    33241 agcggtcttc gtattcgccg gctttttgat gttgttcttg ccgggccagg gcatcctgac
    33301 catgctcatc ggcatctcga tgctcgactt tcctgggaag cgtaacatcg aagccaagct
    33361 gatcggccaa ccgaccgtat tgggtgtcat caacggcatg cggaagaggc gtgggaaacc
    33421 gccgctcatt ctcgcgccgg actcgtaacc gaaactggtt gaattctatt ccgacctcaa
    33481 gaccttatgt cagaagctgc tgtctccagc cggccgaccc tctacttgat tgacgggagc
    33541 gcgtacatct atcgggcctt ttttgccttg cctccgttga acaattccaa aggcctgcaa
    33601 accaatgcgg tctacggctt taccacgacg ctgatgaaga tcatccgcga acaccagccg
    33661 caaggtctgg cggttgcctt cgacgagccc ggacccacct tgcgacatga gacctacaag
    33721 gcctacaagg cgcaacggcc gcccatgccg gaagggatga gtggtcaaat cccgtatatt
    33781 catcgcatcg tcgacgcgat gaatattcct gtcgttaagc tggccggcta tgaagcggat
    33841 gacctgatag gcaccttggc ctgtcaggca gagcgtgcgg ggtacgacgt ggtcattgtg
    33901 acgggagata aggacatgtt tcaacttttg gcgcagcaca ttcggatcta cgatccggtg
    33961 aagagcaaat ggttcggaga agcggaatgc cgtgctcgct tcggcgtcga accggctcgc
    34021 gtcgtggaag ttatgggcct gatgggagac agcaccgaca acattcccgg tgtcagaggg
    34081 atcggcgaga aaaccgccat caaattaatc acggagttcg gcacgatcga cgagctgttg
    34141 cgccgcctgg atgatgtcac cccccctcgc atcaaaactc ttttggtgga acaggccgac
    34201 aatgcccgac tcagccggac gctggccacc atcgacacgc aaagtccggt cgagtttcag
    34261 gccgacacct accgggtcaa atctttgcat catgacgtat ttgccgatct cctgcgcgag
    34321 ttggaattta cgaccctgct caagaatctc cagccggcgg aacgtcatgc cgaaccgaaa
    34381 gcggtaagcg tcgtgtctct tgaagaccac ccttcgatcc atcgcttcat cggcgagctg
    34441 cctccaggcg ttccgctagg ccttcagtgc ctgctcgcag gcggatccgg catgcaagcg
    34501 gaagtcctgg gacttgcgct gtcagtgggg gacagcacag ggttcgtgcc gctcaaatca
    34561 ggaccactgg agaactcctt ggcggcgctg ctgcgcgaca gtggtcggca gaaagtcgtt
    34621 catgatctca aggccgcact cctggcctgc agccgggcgg gactcatcct gaacggtccg
    34681 tacgtcgata ccatggttgc cgattacctc ctcaatccca accgccgcga tcatcagctt
    34741 gagacgatcg ctctggaggt gttgggtgaa cgactggaga agacctcaga ggagaagtcc
    34801 aagcaatccc tgttcgaggt tgaattggcg tcgcgggaag atgtggccca atccgctgca
    34861 gccttggcgc gtcttggccc cgtcatgact caacggatag cagaacaggg aagcgcgaag
    34921 ctctttgccg aggtcgaaat gccgctcgtt ccggtcttgg tcgagatcga gcgaaacggg
    34981 ttcttgttgg acgtcgaaaa gctgcatgcg ctcgggaagg aattggatcg agaactcgaa
    35041 cgcatgatgg acaccattgc cggtttggcc ggaggagaat tcaacatcaa ttcgccgaag
    35101 caactcgcca tcgtgctttt cgaaaagctc ggcctcaagc ccgtgcgaaa aacaaaaacg
    35161 gggtattcga cggatgaaga taccctgaca caactggccg ctcagcacga actgccttcc
    35221 cagatcctga attgccgcag tctgagcaag ctgaaatcca cgtacgttga tgcgctgccg
    35281 caattggtca atccgcacac gcggagactc catacctccc tcaatcaaac cgttgctgcc
    35341 accggacggt tgtcgtcgac cgagccgaat ctccaaaaca ttccggtcaa aggcgacttc
    35401 gggttgcgga tccgggaggc gttcgtggcg gccgacggac acgcattgct ctgtgccgat
    35461 tacagccaga tcgaaccacg cattctggcg catttgtcgc aggatccacg gctgctggcc
    35521 atttttgcgc agagcgagga tatccacttg gccacggcga tggagatctt cagcctgccg
    35581 gccgatcaga ttacgaaaga gatgcggcgg gcggcgaaga ccgtcgtctt tggcattgtc
    35641 tacggaatca gtcctttcgg cttgtcgcaa aatatcggcg tcccacaagc agaggctaag
    35701 aaatatatcg aaacattttt tgaacgcttt ccggcggtgc gtgcgctcat ggatcggaac
    35761 atcgccgaag gcaaggccaa agggtacacg acgacgatcc ttggccgtcg ccggccgatt
    35821 ccggaattgc aaagtggcga tccggtccag cgcagcttcg gcgagcgcat ggcggtgaac
    35881 agtccgatcc agggctctgc ggcggacctc atcaaagtgg ccatgatccg ggtccaccag
    35941 gcattgcagg aacagcttcc gcagacgaag atcatcctgc aagtccatga cgaactgatc
    36001 tttgaggtgc ccgaagcgaa cctggaagct gcgaagcggc tcgtcaaaac ggagatggaa
    36061 gcagtggggc agacgctcgg cctttccgtc ccgctcaagg tggatctcgg cgtcggcaaa
    36121 aattggcgcc tcgcgcatcc ttaatgcggt cctgctcgat ctcgctgcgg actgacgttc
    36181 aaacctgtgc cctctgtcgg gactctcctt caggtcaccc tgtgcttccg cttctttgcg
    36241 cggagttagg ggtggcactc ctcttgccat gactctctcg tttcctcgtc gaccgacatc
    36301 cagaaagaaa gggactcaca tgatccatgc tgtggaattg gacagccaga cttcttcgtt
    36361 tgtgcggcaa ctggaagagc agcggcgaat tgaaaaaaat ctgatggttg aacgcgtacg
    36421 gggctcaaaa gaaggccttg aatccgtctt ccacgacatc aaagaacggg tcgatgcgct
    36481 tgagcggatg accaactcgg tgagcaccag ctatatcgcg cccatcggct ggagtcagtt
    36541 gatcttcgca gggacggacg gtttgtccgc aggtccgacc atggggttcg gtgatcgcga
    36601 tgacagcatc tgcaaacacg atcgaattcg gtatctcgaa gacgcaaatt atctgcggct
    36661 gagtgccaat cctcattacg tcgagcgcgt gctccccaat aagtcagcgc acgcgcgcgc
    36721 cgccgctaat ccatcgacat gggacaacct tggctacgtg cacgtcggct attcgaccgc
    36781 gctcattctt ccggcctctc tggaaccgac cacggtctcc atcctgtcga gggtgaacac
    36841 gctgttcaat tgggtcgaag tcaatgccgt ggcggacgat tggtatgctc atgtggcggc
    36901 gaccgcccgg ctgtgggttg cctatgatgg ggaatttcgt cagacgccgc cggcgcaatt
    36961 tgtcaacctt ctggcgacgc cgaagcaggt caagactcac caacatatcg attcgctggc
    37021 gccgagcgca gcgctggcca tgcagctgga ggtgtcacct gggagcggga cgaccctcat
    37081 gatgtatgag accattgagt tggtcgcgac cactcccaac gcacatgcct atattgacgg
    37141 agtgtttgcc tgggaaccgg tggcggttca gttgcggtga ccagggtctg atggaacact
    37201 aggactgata gaacgtgtcg acgatctgtc cggtgttgaa cagcatggca tcacgcctgg
    37261 tgtaggcggc gtggccgtaa taatgatcgc gcggattcgt ggagccggtc gagtctttgt
    37321 agtccacaag caaacagcgg ccttcgctgg ggcaacggat cgcgcgcctc gcacattcca
    37381 gccagcgctc gaagccatcg aatggctcca ccaattccca ggccgcctta ttggcggcct
    37441 tcgcctgctc ggttgccggc agcgcgtcgc tgccagcgac ggcaagttcc tgcacatagt
    37501 caccccccca tgcgatcggc atctccatcg tgatctgagg caactccatc tttgcaagcc
    37561 aggtcttggc atctgtacgg agattgcgat ggttcacaat atgaaggagc ttcccgattc
    37621 ccgaagggtc ccatccgtag cgcaccggct gcccaaaggt cacgatgtcc aatgaagcgc
    37681 cgttcagcag cgaacccgcc gacagcagcg gagcgagcct gttgaccact gctgccaagt
    37741 ccggttggtt ggtctgttcg gcataggatg tgaggatggc aagcagcctt ggtctcccgg
    37801 tgatagcgct cgggcaaagg aggttcgagg cgagtgcgag gacgaggccg gcctggccat
    37861 gcgcttgaat gaggatgcga tcaccggacg ccagctgatg caactcgcag tgcatgcgaa
    37921 gctcatcgag catacgcacc gcggccacag ccctcccaag gtgatgatgc tccgatgacc
    37981 acaagaggcg cacgcatctc aacggtgtgg atgctttgtt cagcgctcgt tgcagcatgc
    38041 caattgcggt ttctgtaaag ttcccggcgt cgccggcgtg atggtcgatg agctgcttcg
    38101 ttgcgtcgtc gttaatgagc ggaggtcgaa gcccgccggg taaaagcggg atcccattcg
    38161 tttcctggcg catggcggcg aggagcgcat cgagtcccga tacccctcgt gagtagcctc
    38221 gtttgagccc ccctgcctcg tcgaggcgct gggtcccgaa gacatcggta ccgtggatcg
    38281 caccgtgtac gaacatgacg gtgcgcaccc cggcacgacc catcgccgac ccgagtttga
    38341 ccatttccgc cgtccactcc ggagagtccg gctgggggcc cgccgtaaac tccctggcgg
    38401 ctaaccgctc tcctggatgt tttctggaca gctcgtcatg ctgaaaattg ttctcaatcg
    38461 gcatcgtatg atttcatact cgatctcggt cgcgcactgc aatggaagga tagttagcgg
    38521 gtaatgacag gcgtcgattc ctgacgttcg atgagaggct ttggctgtaa gatctgacgt
    38581 ttgggcatta ccgcggtcgc agagcagatg ttcctttcta catcaattcg acggtcgacg
    38641 atcttcacgc cttgaaggta ttcctgcacg atctcttcac gagtcaattc gatgtcctga
    38701 tcgacgcctc ttcctgaccg ttcccggacg cgatcaatca tgtgctcttt gactaagacc
    38761 ctgatctgct tggccagatc agtacgcgca gacaattcgg acacccgttg acataccagg
    38821 gcacctttcg ccatatcgcc ttggcctttg cctatcacaa attcatcggg tgaaaagccg
    38881 gcttcattgc tgtggccggg tgattcattg taggagacag tgaagccagt tggctgttgc
    38941 cgaagttttt ggaatgagtg ctcggcgtga agcctaacgc gccctgactc agagacaaga
    39001 ggagacatcc acgctgttga aggggttcca agcgccagag ttgccaacag aagcggtaag
    39061 acgttcgtca ccgattatct tccctttcca cccgcccgcc cgccgcctgc tcgtccgaat
    39121 ccgtttccac cacgtcctcc tcgtccgtaa cggcccggac cttctgaaac ccgaggaaca
    39181 ccatagtggg gcagatgcgg gaccaagagg ggcgattgaa tgtgatgagg tgaggtcggc
    39241 actgcccgcg taactggcgg ctgtcctgtc ggagggacgg tggccggtgg ttggaggaat
    39301 tgtctcagcg actgaatgga tcgggagccc tgccgaaggt cacgtgatcc tcttgaaggc
    39361 gcaatggccg tcggggcttg taagaattgc tggagtgatt gaacggatgg caaggcgggc
    39421 ggacgaggct gcccgcctgc cggtgcattg ccagaagggg gcgaggagag cggagccaac
    39481 tgtcgcatgt ctgtgaaggt ccgggtgccg actcgagtcg gcagagtaat gccgcccggc
    39541 tgattgccga gctgcacggg catcgtcggt gttctatggg aaatttcgtt gagtggtaaa
    39601 ggagtcgttc tagcgccggg ctgaaccggt atcacatgag tggtcgggac aatggtctgc
    39661 gtggctgcgg ccggcgtgct cgccaccatg acacgctggg cggtcgggac atgaggacta
    39721 cctggataga gttcccgcgc ctttgtcaac agcatgtgcg aaggtgtgat accgaacaac
    39781 tgagacggat aggtcgcagg tccgatcatg acccagtccg tccatgcttg agaaaccatt
    39841 gcatgtgatt gcactcgatc caacagttct tgccgctgga ggctggcttg ctgagcctgc
    39901 tcggccgaga gggcgacggc gagcgattga gcggccactt ccagttcctg atgcaggtca
    39961 tcgatgtctc gttttaatgc cgcgagttgc tgtcgcgcgt cctcattttc acgaagggca
    40021 aggatcgctt gcgccacctc ttcagtgtca atttgggatg tcagatcaac agtgatgatc
    40081 acggcgtcac cgtcgagcga cagggttgtc tgttgatcgt gaaccacaac cagtccggcg
    40141 gtgtaggtgg tgatctcgtc cctggtgacg tcgacacctt ccacgaccgt aatgctttcc
    40201 agataggtgg ccacctgttc caacgcattt cgtttggcgg cttccgttgc caggcgcatg
    40261 gcatcttcct gcgtatcgcg ggcacccatt cgatatgcac cctgggacaa gacgattcgc
    40321 gtctcggcgt atgcgatctc accgagacac catagaagga ttgggaagca gagaagcgag
    40381 aaaaggagca gcaaagtgca gcgctgtacg cacgagagat tcaggcagcc aggcgggtcg
    40441 gacatggtcg tcacccttaa tccagaagac tgacgcttca cccgtgccgg cgctcagcat
    40501 aacacccggg aaagccgttt cgccagcgct tccccgcctg cggcacctgg cttgccttca
    40561 agaaaattcg cgtgctaagg taaccattcc ctttttgact tgaagcggag acgcacgcag
    40621 atgagatgga aaaagagctg ggttggcgca ctcacgatga gcctgtgctt ggtctctatt
    40681 atggtctcct cagcggctgc tccactgcgc ccttcgtccc gcgaggggaa tgcgattcca
    40741 acaagtcctc cgactgaaca tgtcatcctc tttgtgctcg aaggattcag ccaagactcc
    40801 ctcaagggcg gcacgatgcc cacactgagc aagttagtca aagagggggc ggtgacctgg
    40861 tcagcgagcg cagtcaaacc cgccctgcga ttgccgacaa tggcgtcgat catcaccggc
    40921 gtccccgtcg agaaacatgg cattcactgg aacaccttcg agtttagccg ggggtatcct
    40981 cgagcaccca gtgtctttga ctacctggat ttgagcggcg gaagagacag cgccatcttt
    41041 tttatggacg agtcgctcta tcaattggca cgaccggagc cctatacgga ttatcagatg
    41101 tgcggacccc tgaagcccga gtgcagtccc gatcgcgtgg tctcatacat tcagcagtac
    41161 ttcaagaagg cgacgagcgg acatgggtac ggacacgcga ttctctcgct gccacatttc
    41221 cttgtcgtgc accttccgga ggccggacgg gtcggcgcag cgcgcggatg gacttccaaa
    41281 gagtaccgtg aggcgctccg agcggtcgat aaggccatgg cttccgtgtt ggacatctac
    41341 aagagcgagg atctcacgaa gcggaccacg gtgtttgtga cctcattgag cgcggaaggg
    41401 atagacgcgg gtcatgagca ggccacgaat ggaacggctc cagtcgtgcc ctggatcgcc
    41461 tccggcgtcg ggatcaaaca cgggcacgcg attcatcagc acgtgtcaat cctcgacacc
    41521 ggggcgacag tcatgcgcac cctaggcttg cagacgcata cggagtggga gagccggcca
    41581 gtcgaagaaa tttttcagtt cgccttcatc gcggcggcgc ccaattcgaa tgtgccgtag
    41641 ggatgaagtc atggacagtc ccggcgggct cgacgcatgg cggacgctcc tcgtcagcgt
    41701 gtcgttgttt ctgtgcgcag gcctcgtgtc gctgccggtc agcgaggccg caccgccgcc
    41761 gagttacacg aaagagcacg ccatcatgat cgatccgaca gtactcgatc ctccatcttg
    41821 gtggtacgtg ccggggatca cgccatctat ctacaactcg gaccctgatt cgctggatgc
    41881 ctataagaca accgataagc ggacgctcgc attgaagccg gggaagtaca aatttatcag
    41941 ctttacgttt gatttccctt tttccgtgaa tctcgagggc aaattggatt ttgcgccatc
    42001 gctcgatcaa tgcgtggaag gacgcgggac acaaaccttg gttgtccgct gcaagcggac
    42061 atacccctat ggtggacagc ctgactatac ctatcagtga gtctggccat ccggcgcatg
    42121 caggtgatga aaagctgatg agtccggtgc tgtgttggcg accagaccag atccgatcaa
    42181 gatgaaaagg acatgaagat gacgcaggcg ctagacgatt tactggaagc actcgaggac
    42241 gtcgacgatg ccactcgtga agaagctgcc aaggcgctcg ggacgatggc cgatcccaag
    42301 agcttggatg cgctgatcgg tgcctgtgag gacgaatatt gggccgtccg tgcacatgcg
    42361 gggtcggctc tggcgaaaat cggaggccag cgggccgctg atgcgctcgt cggcttgttc
    42421 aatgacccga tcatggaagt acggaaccag gcggtcgaag cggcggcgac cttgggtgtc
    42481 ggggtgctgg accggatgat aacggcattg aaggacgagc gctggcgggt gcgggagcat
    42541 gcggccaaga cgtgcggtga catcggtgat cggaaagcag ttgacgcctt gatcgtggcc
    42601 tgtcgtgatc gtgacggtgc cgtcaaaagt gccgccgcag aagcgctggg caaaatcggt
    42661 gatccgagag cggtgccagt catgattaaa ttgtttcgcg atacgtccaa gatcgtgcgc
    42721 gagactgccg gcactgcgct ggtctatatc ggacagcctt ctgtggatcc gctgatcgaa
    42781 tgtttcaagg acaaggattt tgtcgtgcgc tgccatgccg cacgggccct gggcgggatg
    42841 acgaccgact accaaatcgg caggacatgg gtgcgtgagc cgaaagttgt tgatgcgctc
    42901 attgccgcgc tgaaagatcc ggatcgcgcc gtccgcgaag atgcgacgat cgcgctgggc
    42961 atgatcggag atcctcgtgc catcgatgct ttgatcgaag ccatgaagga cggtgcggtc
    43021 aagcgccatg ccatcgcgtc tctcggcatg atcggtgacc cacgggcgct gggacccgtg
    43081 ctggatgcct tgaaagggaa agggatccgg caggatggca cgccgacgcc gggctgcatc
    43141 gtcagcgagg atgcgttcat caaagaagca gcggctacgg cccttggtca attccgtgat
    43201 ccacgcgtca ttcccgatct gatcatgttg ctgaaggacg gagttctgcg tgaaaaggcg
    43261 agtgctgcgc tggccgcgat tggcgatacg gcgatcgaac cgctcattgc atttctctac
    43321 gatcccaagg cgtcggaagt ttcggccgag ggcgaacggg tgctttccta tgcttccgtt
    43381 cgactcagcg cgaaggacgc cttgcggcaa ctcgtcatgg aaacccttga aaagctgggc
    43441 tggacattgc cgcaggaaga tgtcgcagtg aattcaagct cagtggacaa tgcgcgcgtc
    43501 gataagcccc tcggtgaggc gggacgcttc ggcccagccg gagacctcgc caaatagtgt
    43561 catgagggcc tgacctctgc cctgaccgat cttgcccgcc cggtcaatgg aggagggacc
    43621 tgaaatccac aaacataata ggcatcgggt gaaccttcgt gagatccggt gctgaaccaa
    43681 atctaccgac tgcacgcagg ttgcacgagc gagcctacct ccatgttatt tccggccgaa
    43741 tcgatgctcg agcggtaccc tccgcgtgga cgcgtcagca tgcgtcagct ttgtcgttga
    43801 cgcatcggag aagcggatca ccagtaatga acaccccaca ctacgaagat caagtcccgc
    43861 gaggaacgac gtcgccccgg cagatgagga ctggtcgcgt tgaccctgtg aaaatggcta
    43921 tgttaggctc accaattccg aggaaattcg cctatggccg attccgtagc cgagcagatc
    43981 gccgcactgc aggacgacga ctgggcaatc cgcgaggaag ccgcagtggc attgggagcc
    44041 tttcgcgacc ctcgagcagt ggggccgttg gttggtcttc tgcgtgacag cgatcgggcg
    44101 gtgcgggaag cagctattgg cgccttgacc gcgattggcg ggccctctgt cccggttctc
    44161 ggtctctgtc tttccgatcc gcaattgggt gttcaggaag ccgcttcgtc cgtcctggca
    44221 tcgatcgccg acgaacgggt gatcgatccg ctgatcggcg ccttggcgaa tagcgattgg
    44281 attgttcgta tgcacgccac gaaggcgttg ggacgcatca aggccgcggg cgcaatcgac
    44341 ccgttgattc cgctcttgca agacaaggtg aaagccgttc gggaagaagc cacggtcgca
    44401 ttggcgaaaa tcggcgatgc agcgctttcc tcactgctgg acgccttaaa gcatcctgaa
    44461 tggttggtgc ggctgcacgc agtggaggcc ttggcgagga tgcggtcgcc acgcgcagtc
    44521 gaaccgctgc tttgggtatt gttcaatgat accgaccagg cgatccgaga agatgtcatc
    44581 cgagccctgg gccagatcgg cgacagccgg gcgacggaat ttctcgtgac cgccatgaaa
    44641 gagcctcgcc tgcgcacgct tgccgtggaa tccttgggtc aaatcggcga ccgctcggcc
    44701 gttccgctct tggtggccgt tctcgaaggc gccgatcgac ttgagggcct gcgtccggtt
    44761 gccggatgtg gcgacaggtg ggacgaggaa atggtcacga aaggcgccgc ggtgcgcgcc
    44821 ttgggcagca tcggggacga taccgccatt cccgccttat tgaaggcgct agatcagacc
    44881 gagacccgag ccgatgcggc cgcggcattg gtcaaattcg gatccaaagt gattgcgccg
    44941 ttattgacga tcatgacgca atcctccgat gagaacgtac gcttccatgt caaagacact
    45001 ctggcacaag tggggtggcg ggccggccgg atttaactca atgatgatga ggctggttgc
    45061 gtctgaccga aaagcctgat ttgctgatcg tgagacgcga tgtgagcccg atctatgtcg
    45121 aaggaaacca tcgagacgct cgtagcagag ttggtccatg aagaggactg gcggcgcatg
    45181 cgcgcgacgg ctgcctgctt ggccggaggt ccgagagccg tacaggcgct gatcgaggtc
    45241 ctacgttcag gaacgtccga tcttaaacgc gaagccgccg ccatgttggc acgaatcaag
    45301 gatccccatg cgggcgtcgc gttggtcggg ttgcttgaag accacgatga aggcgtccgc
    45361 cgggcgggtg ccacggcatt agagcagatg gccggggtgc tcgacccaga cacggctgct
    45421 gccttggtcg cgttgctgcc aaagacggcc gagggagaca ctcgcagact cttgtcgcat
    45481 ctggtcggag cgatcccgac ggcagtcgct cctctatgcg agatgatgaa gcagggggat
    45541 cccgaagcgc aagtcacggc tgcgaccatg cttgatcatt tactggatcc ccgatccgtc
    45601 gacgcattca ttgatgcgat ggcgcaccct gcagtccggg acatcgccgt ctggaccttg
    45661 aagaagctcg gggcgattcg agagcgcatc gatgactcgt tcaacgcctt gcgcgatgtg
    45721 gaaggcgcca gtgaacgtga agaggcccgt atggccacgg tcattaactt gcttggaatc
    45781 ggccgcccga gtgttgaaat tctgatcgaa tacctggaag acgacgattg gttggtgcgg
    45841 gaagcggcgg cggacctcct cgggaaaatc gcggatgtac gcgcggtgga acccttgatg
    45901 aagcgtctgg agcaagacaa ggacacagga gtgaaggagc tcgcgatcaa agcgctcgga
    45961 ttgatcggcg atgcccgtcc cacacgactg tacctcgagg cgattccgat tcgcccgtta
    46021 cgtgtgtatg cgatggaggc cctggccaag atcaaggatg tcgaggtgct gcgtcctgat
    46081 aaagaactct tcgaccggct acgcacggat cgtgacgggt tggtggcgta caatgctgga
    46141 ttgatcgccg ataaactgga agcgatcgac agcgcccatg caatcagccg ggaaggacat
    46201 ggcgatgagt gacgaaggcc ggtcggaacg ggtcgagcaa ttaatcggcg cattgcgtga
    46261 tgacaacgaa gccctccgtg accatgccat tgcaagcttg agccaggtag gcgaggaagc
    46321 gattggccca ttaatcggct tgatggccga cgaggatgtc ctgatccggg aagcagccac
    46381 agtcgcaatc gtcaggatgg gcccttccgt cgtggaccgc ttgatcgaag cgatcgaaga
    46441 tgacgaatgg gccattcgcg agcaagcggc gtccggtttg ggaaagctca aagatcctcg
    46501 ggcggtggaa ccgttggtga aagccctcaa ggataaggac ggggcggtac gcaccgccgc
    46561 cgtctgggcg ctcgagcgga tcggtgatgc tcgagcagtc cccggcttgg ttgaggcgtt
    46621 gacggacggc acggttcgcg aggatgtcgc acgcgtcctc aagaaaatcg gcgatgcgag
    46681 ggcagtggat gccttgatcg acggcttact gggaaacaat tggatggtca gacgccatgc
    46741 agcggaagcc ttggggaaga tcggtgatgc tcgcggcatt gagccgttga ttgaatctct
    46801 cagagacgaa gattggctgg ttcggcgcaa cgcggccgaa tcccttgcca ggctgggagc
    46861 gaagcaggca attgatccct tgctcccatt actggaagac gaaaatacga tggtgcagga
    46921 aacggtcgaa ggggttctag caagtctggg ttggaaacaa ggggatcagt aacatcattt
    46981 ccggatgtca ttgtaaagga acgagcacat ggcagatgaa gctcccgtga aactgattca
    47041 aatcggcccg aaaggcgggg cgaagaaaga cggcttcaac ctggtcactg aacgggtggt
    47101 agccgtcaat cctgaggcca aacaggtcga ggtcgaactc ttggcctacg acggcaaaac
    47161 cgtcgtgctt gatgtcgacg aggcggcgct cgaagaactt cagcggctga aggccggcga
    47221 cggcgcgacg atccgcgttg tcgaagaggg cggcaagcga attgccaaga gcttcaggat
    47281 ccgagccaag gatcctgatg ccgccaaggc tgacgcgatg ctgctggatc tgaaggacag
    47341 ccattggctc aatagaaaat atgcggcgga agtcctaggc gaactccgag aaccccgggc
    47401 cgtccgtcca ctggtcgacg cactcaccga cgaagtcggc gacgtcaggc agcgggccta
    47461 cgattcattg attaagatcg gcggtgtggc ggttccaaca ctcgtcccgt tgctggtgtc
    47521 cgaggaggat gagttgcgac agtcggcgac ggagattatc agaaagatcg gcaagccggc
    47581 ggtcgaaccg cttgcaactg cgcttgcgga agccgatgat cggctgaagg ctcgcgtcct
    47641 gaaagtcctg gaccggatgg gctataagcc gaagaccaaa gacgcggtga aagccgaaac
    47701 gcctcgactt actcagtcgc cgggctagcc gggattctta gtcggacgcc cgacagacac
    47761 atgcctgaag ccgaattgag tcacgcgact ggattcatag acattgcgta ggtcgatcaa
    47821 aatcgcccgg cgcatcaccg acttcagttg gtcgaagtcg agatttcgga attcattcca
    47881 ttccgtcatc aggactattg catcggcacc gcgtgcggtc tcataggcat tttgacaggg
    47941 tgtgatccga ggcaacagct tggagccttc ctcaagggca gcaggatcat aggcacgaac
    48001 ggtgaccccc tggtccataa gcagctgagc aatggaaagc gcgggggctt cacgcaggtc
    48061 gttcgtgttg ggtttaaagg acaaccccaa gaatcccacc gtcgttccct ctaatccgcc
    48121 gagcgcatct ctgatcttgt ccaccatgcg aacgcgttgc tccgcattga cggctgccgc
    48181 agcgccggcg atttgcatag ggtaaccggc cttctcgccc atttgaacga gggcggcaag
    48241 gtcctttgga aaacaagatc caccaaatcc gggaccggca tgaaggaact tggacccgat
    48301 gcgctggtcc aggcccattc ccttggctac ggtttgaaca tccgctccga cccgttcaca
    48361 gagtgtcgca atttcattga taaaggagac tttggtcgcg agaaatgcgt tcgaagcata
    48421 tttaatcatt tctgccgtgg ccacatccgt gacgacaaac ggagtttcaa tcaggtagag
    48481 ggggcgatac aaatctttca tcagggcaac ggcacgctca ctgtccgatc cgatcacgac
    48541 gcggtttggc ctcataaagt cttcgatagc cgagccttcg cgcaagaatt ccggattcga
    48601 tacgacatcg aaggcgacct tcctggtctg tgtaccggcg atgacctgtt gcaagaacgc
    48661 gcccgtcccc acgggaaccg tcgacttggt gacgaccact ttgtaggagg ccatcttccc
    48721 ggcaattccg cgtccaacct cttcaacgaa cgagagatcg gccgagccgt ccttcttagg
    48781 aggtgtgccg acggcgataa aaatcacttc accgtggtca actgcccttg taagttcggc
    48841 cgtaaaattg aggcggcctt ggccgatgcc tttcgcaacg agttcggtga tgccgggttc
    48901 atagaagggg acgtcccctt tctcgaggcg cgcgatgcgc ttcgcatcgt tgtccatgca
    48961 cagtacgttg acgccgaatt cggcgaagca ggcgcccgtg accagtccca catacccggt
    49021 tccgatgaca ctgatctgca tacgtcgtta cctcctctac tcgatggacc agcgcagtat
    49081 actctgcgtc gttgtctcgg gcaaagagga ttcatgtata cctgcagcca cacgcagagg
    49141 cacgcttcgt tgacagcttc cagaccaggt ggttactatg agcgcccctc gcgacacatg
    49201 cacaacaaag ccgccgccag aagagcattc atgaaacccc ttctttctgc atgacccagc
    49261 acgtgccaat gcaccatccc agttggttcc tgtttctcac cgcgttcatc acgggtgccg
    49321 tggtgatggc gctggaaatt ctgggtagcc gcctgctggc tcctgtcttc ggcaactcgc
    49381 tctttgtgtg gggggctctc atcggagtca tcctagcggc gatgagcagc ggatacgcct
    49441 tcgggggttg gatttctgat cgtctcccag ggggtcatgt gctcgcggga cttctgctgt
    49501 tttcgggatc gtggacgttt ctcatcgcat gggcgagcca gccaattctg ttcaaggtgg
    49561 cgggtgtgat cgaagatccc aggtggggac cgtgtcttgc ggccaccgtc ttgctcgccc
    49621 cgccggcatt tggattaagc ggcgtgttgc cggcgatgct ccgactcgcc gtctccgatt
    49681 tgaactatct cgggcgacat accggcagga tgatcgccct gtcgacagtc ggaagcctgg
    49741 cgggcacctg gggaacggct ttttttttcc tgtcatggat aggtagccag gccttagtcg
    49801 cgtggctcgg tgtcatgcag acggcactcg gattgtggtg gctcgtcaaa gggacggccg
    49861 ccaaacgggt gaccgtggtg tgcttcgcga tgatcattgg agggctggca gtcggtgcgt
    49921 ggtctcccat ccacatgctg aagccgccgg tctaccagga agacagtccc tatcagcagg
    49981 tcagaattcg agaagacgat ctgtttcgct acctcgtcct cgaccgaaca tttcatgccg
    50041 tcatgtggaa agctgaaccg gtcactctct ttctgccgta tagccagctc atggtgtcgt
    50101 ccctggcgct ggtgcctgag gcgaaacggg cgttggttct gggccatggc ggcggatcct
    50161 ttgccaagtg gctcgcccgg tattggcccg atttggacct ggatgttgtt gaatttgatc
    50221 ccgtggtcgt caggatggcc gaagactatt tttcctaccg tcctccttct cgacatcatg
    50281 tgtacgttcg tgacgccaga gcctacttga acgataccgc taacacgtat gatctgatct
    50341 gggtcgatgc gttcgctcga cacctcgtcc cgtttcatct gacgactcaa gaattttttt
    50401 cagacttgcg aaagcatctg acgccggagg gtgtcgtcgt cgtaaatctg gcggcatccg
    50461 ggaatagggg cgatctcgga agagccaaag cggtggtgca aacgatgaag caatccttcc
    50521 ccgtcatcga gacctatgcg gtcaaaggac catggccgtc caaatcacgt gccgaaaatc
    50581 ttttgttctt tgccggcgag cccgtcgaac acaatacggg atctgatttt gccgccaagg
    50641 ttgctgcact cgttgaacgt cagcgtcttc cctcggaggc gatcgccttg ttgtcgaccc
    50701 gtcggatgga accctgggaa tcaggcgctg tgttaaccga tgattacgcc ccttatgatc
    50761 tgctcatcgg aatggaaaca ggtgaagatc agcctggagg cgatcgctag caagacattg
    50821 attttgaatt gattgtcttg tggacaaacc agacagggtg accggtggtt gctcggaact
    50881 cgcgtcatga acgatgttgc ttccgttttc gagcctgtgc tataaggttg cctcccaatt
    50941 tagagtacca aagtgggcgt tcataacgcg gatcagatca acgggtgtcg gaagggtcaa
    51001 ggctacctga tcgtggcggt agttatttac caacctaatc acatctcttc ttctttttaa
    51061 ggaggtaggt catgggcaag acgatgttat gggtcttttt cggcctcata atggtcatgg
    51121 caggcgcgtc cccggcgctg gcgcaccagg caggcggagt cgaagttggc gatttggaga
    51181 ccggatcggt ggtcggtcag ccgttcaagg aattggatgt ggacgcagag ctctatgcca
    51241 ttgagattgg caacctcaaa acgtggtatc cgcccgtcac cgcaattgac ttcaaagcgc
    51301 gagccggccg gcctgtggtc atcaaggtga cgaacagttc agcggcagag cacggcttct
    51361 tgctgacggc agaggaaacg cagtcggctc ctacagtgtt gaaggctcag ttggtcctca
    51421 agcccggtga gaccaagtat gtgggtatcc caaccagcga ccttttttat gcgaccactg
    51481 gaaacatcct gaaataccgc tgccatctgc accccgcaca cgtaggtggt aagctgatta
    51541 tgtttaagta gtcgtcgaac gcgagagatg atcagcgccc ccgctgcctt gacggtggcg
    51601 ggggcgctgg tgttttatca cgcttcttcg gtccggcagt ctgcagcagg ctcaagcggg
    51661 acgttttggc tccccttcag ctccccggcc acgatcaaga gaagaaatcg actcaacgtc
    51721 ggctccgctt cccgtcgttc cttcacgatg gcctgaacgt cgacgatcat ttcatctcca
    51781 tcccattgcg tttgattaat gacccagtag tgactggccg catcagggaa ttgtcgaacg
    51841 acaaactttt cgacgatgct tccgatgtct ccggtactgg cttgggatgg ggagggccac
    51901 gtgcagatca cggaccacaa gagagttccg agcagaatcc atcgagagcg catagctcac
    51961 ctcgcgcatg atggctagat cggctccatt ggactaggtg ggcctgttag gagccgcgat
    52021 gtgggatcta agtgggattg tactgcttga gccgggacaa agctacccaa agcgcaggat
    52081 tcggcgtcat gaaaaagtca tgagccctgc ttctacaaaa gaagcaaggc tcgtgaccgt
    52141 cgcgatacgg cattagttcc gaacgccgct ccaaaccttg gcggcatcga tggccttatc
    52201 gggattcagt ttcttggcct tttcaagcag aacgtccgtg gtctcgggat aggcgggatc
    52261 ggaaatgcaa caattatcca caggacagac agcggcgcat tgcggttcgt caaagtggcc
    52321 gacgcactcc gtgcagcggt catgggtgat cacataaatg ttatcgccca ctccttgccc
    52381 gtcgccgaca tggttgcctt tcgtttccgc atcgctgcga gtttcaaaga tcgcttcgtt
    52441 cggacattcg ggtaggcagg ctccacagga gatacattcg tcagtaatca gcagtgccat
    52501 gaccctcgcc tccttctcac tacagctaga aaccaagttg acaagattgc gacgccacga
    52561 catagtgcgt gacgtgaata aggcgtcatt ctagtctgcg gctcttttcc aagtcaacag
    52621 aacggcgtct gtgcagaagg cctagatccc ctgttattat gggcctttgg gcccagattg
    52681 cgcttgccag aaagcgattt tatgacggtc acttcggccc aacgggatca gaagaaaacg
    52741 cggatcatta tgatgatcct gcttgccgtg ctgctcatca gtgtttccat gttcctcgtg
    52801 cagcgggacg attcgaaaac cattattgtg acgtttccca atgggactca gatcgaggca
    52861 gaagtcgccg atacgccaga aaagttgctg ttcggattgg catttcagga agcattgccc
    52921 tcgaatggag gaatgatcta tatctttgag tccacaggac accatcgcgt gaccactaaa
    52981 gcctaccgct ttcctgtcga catgatctgg gtcgacgaaa gtcgccacat cgtctccgtc
    53041 atggagaatg tccagccttg tcaggagggg acttgtccat ggcatgggtc gacttccgat
    53101 gacgtgcggt acttgattca aactgaggcg ggtttcatcc aacgtcaggc cttgaccgtt
    53161 ggcggcgagt tgaaatacac actgcatatg taagcgtcgg gtggagcgct gagctgaccc
    53221 cacgaccgga ggcaatgtgg cgattgaagg gtttcaagtg gtcgcgaagg tcgaagacct
    53281 ccctgcgggc caatccaaag ttgtcacgct caacaaccgt accatcgcct tgttcaacgt
    53341 ggacgggaac atctatgcca tacataacct ctgtccgcac gagggcggtc ccttgagtga
    53401 aggccgagtg aaggggttcg tcgtctcatg cccctggcat gacctcgcgt tcgatatccg
    53461 aaatgggcag ggcatcgatg gaggaggata ttgcgtcggc agttatgagg tacgtgtcga
    53521 gggcggcgag atccatgtcg gtgcgcgtcg gaaaagctag cagctctcac gaaacggatg
    53581 acatggacgc tacgggcgga ttacagggcg acggcaagag agtcgatgct acactcgatc
    53641 aaccgatcca cattcatctc tgggaagatc ggacgagggg ggaactctgg attcccagct
    53701 atgacgcgtc cgccttggcc ttgctcggtg acgattatgt cagggtagcg gggaacaatg
    53761 ctgtcgacaa cggtcaacga acgtttgaat tcaaggcgat gaaaccagga acctttacga
    53821 tactgttcga gaaacgcatg ggctggaagt ttacggcgga ggaccggcgc gtcttccacg
    53881 tcaccgtttc gcctaacggg agcgcatgac cgtgcccgag acggcattcc tcaatggacg
    53941 gttccttccg ttggccgagg ccacggtttc gatcgaagat cgtggctttc aatttgggga
    54001 cggagtttac gaagtcatta gaacctaccg cgggcgtccg ttcaaactgg atgcgcatct
    54061 cgctcgcttg gaccgcagtg cccaagccat tgaccttcgg cagccatatc catttgagcg
    54121 gtgggttgag tatgtcacgg agggcctgcg tctgggtgac tttccggaaa cgaagatcta
    54181 tgtgcagatt acaagaggga cggcgccgcg cgatcatgcc tatgcaccag atcttcaacc
    54241 gaccgtgctg atgacgtttc gtgaactgca gcccctgaat gcgaccttcc catccactgg
    54301 cgttgcggcc atgaccatga aagatattcg ctggggccga tgcgatatca aaagcctaaa
    54361 tctcttggcc aacgtactgg ctcgccagca ggccaagaag cgcggagtgt ttgagtcaat
    54421 cctcgtacga gacggccagg taacggaggg cgcagtcagc aatgtgttta ttgttcagga
    54481 cgggaaactc atcaccgcgc ccgaaggtgg ctggatcctc tcaggcgtga cccgccaggt
    54541 tgtgctggaa ttggcccggc acgaagggct catggtgcag gaacgctact gctcggagca
    54601 agagttgctc ggagcgacgg aagtgttttt aaccggaacg actgtcgaag ttctgggcgt
    54661 cgtgcacatc gatggcaagc agattggaaa gggacaaccg ggcccggtga cacgaagtct
    54721 ggccaagcgt tttctcgacg aaacccgata atgggcccct tgctttgaca agggtgacat
    54781 ggtattgtgc cacctctgca aagtgggctc aggcccactt ttttgtttga cgcgtacaga
    54841 aaacatcaag ggaagcgggg ctaggggtgt tggtgaagga gtcccgaccg gtcgtcgatc
    54901 gcatcagcga ggcagtgtcc cccatcctat ggacgttagg cctcgaattg gcggatgtcg
    54961 tatgtgtggg gcaagggtcc cgttcggtcg tgcgggtctt catcgataaa ccggaagggg
    55021 tcacgttgga ggattgcgag cgcgcacaca aggcgctcgg tcccgcgctg gatgtggccg
    55081 atccctttcc ccatgcctat acgctggaag tctcctctcc agggctggac cggcctttca
    55141 agcggcttca ggactatcgt cgggccatcg gaaaacgagt cacgttaaag ctccgagaac
    55201 ccctcgccgg ccaatggcgc ctggtgggaa cattgaccga cgtcgcggat cagacggtga
    55261 cgctgacggt tgacgatcga ccgaccgttc gtgctgtgac gctggagcaa cggttgatcg
    55321 cggaggccaa gcgggccgtg acgtggtaag catggagggt gttcgagtgg agaccgaaac
    55381 atgaaccgag aattaatttc agtcatcgat gaaattggcc gccagaaggg aatcgacaag
    55441 acaaaggtca tcggagcgat cgaggcggct ctgcagacag cggccaaaaa gcgcttcggc
    55501 caggcggaaa atatccaggt cgaaatcgac tccaagaccg gagaaatttc cgtggtctcc
    55561 aagaagatca tcgtcgagtc ggtcagtaat ccgaaagccg aaatctctct caaggaggct
    55621 cgggcctacg acagcgaggc ggaaatcgga gacgagatag gctcgctgat tgaaatggat
    55681 gagctcgggc gcatcgcggc tcagacggcc aagcaagtca tttttcagaa ggtccgggaa
    55741 gcggaatggg aagcggttca gaaagagtat tccaccaggc agggcgatct ggtgaatggc
    55801 atcatcctcg gcatggagcg gcgcaattac ctcgtcgact taggcaaaac agaagccatt
    55861 cttcccattc aagagcaaat tccacgtgaa acctaccgcc gcggcgaccg tgtcaaagcc
    55921 atgttattgg aagttcggcg gacgccgaaa gatgtgcagg tcattctgac acgcagccat
    55981 ccccaattcg tctcgaagct cttcgagttg gaagtgcctg aggtcatgga aaagattgtc
    56041 gagatcaagg cggtcgtacg ggagcctggc gatcgcacga agattgccgt cacctcccgc
    56101 gaaaaggccg tggaccctgt cggcgcctgc gtgggaatca aaggctcacg tgtccaggcg
    56161 gttgtgcgcg agctgcgagg agagaaaatc gatatcatta cgtggacctc ggatccgcgc
    56221 gtgttcatcg ccgaagcatt aaaccccgcc accatcgaaa aggtcggagt cgacgaagaa
    56281 aaaaaatccg ctctggtcgt ggttgcggat tcccaacttt cattggcgat cggcaagaac
    56341 gggcaaaatg tgcggctggc agcccgcttg accggttgga aaatcgacat catcagcgcc
    56401 accgagtacg aaaaagagaa ggctgagcgg gataaagaaa tcaaggcggc gctcgcggaa
    56461 gaagcggaag cgcaacggca acaggaagaa gcacgtcaac aggaaaaggc ccaacaagag
    56521 gcggaagggt aacgaactaa ctcgtatgcg cgtgtatgaa ctggcgaaac agcttgggat
    56581 ggagaaccgt gacttgatcc cggagctcaa gcgattggga attaccgtag cttcccacag
    56641 cagcgccctg gaaccggagg cggttcaaaa ggcgctggaa aagctcgcct caaagcacaa
    56701 ggcatcggtc aagggagccg ggcgacctgg cgagtcggat gcagggcggc agaaggacgc
    56761 agacggagcc caccctccgg caggtgcgaa agcaggagtt cacccgacgg caacggacga
    56821 ctccaagccg gataagcgcc gcatcctgat caaacgaaaa cgagatgagg caccttccga
    56881 ggaactcacc ccgcttggac acgaaccgct cgttgcccat tcaaccgaac ctccggcggt
    56941 caccgtgtcg ccgcttcatt cgccgtcgga aagtccggtg gttcatcacg aaccgcttgt
    57001 ttccgggacg acggcaacga ccgagtcgcc agtggccacc aagccggtgc cggcggcgac
    57061 ccctgagacc ctgacgggca aaaagaaagc ccttgaagag ctccaggctg aggggctgaa
    57121 ggacaagctc aagaaagtgc ggaagccggg acgcgttcgt gaagaggatg aagtgcgcgt
    57181 gcgtgatgac gcggctcgtt ggcaggatct tcgggccatc cccgttcagc ggcgcgatga
    57241 tcgcagcaag cacgtgcatc acagcacgcc ggccgaaata accaagcctc gcaaaaagag
    57301 cgtgaaactg acgccgggta tgaccgtcaa agagtttgcc gaaatgatcg gccagcgtcc
    57361 agctgacatc gtgcggaaac tgatggatat gggccaaatg ttgacattca accagccaat
    57421 gcacgtcgag gccgcgtcaa tgattgcaga agaaggcgga ttcaagatcg agatctcggt
    57481 tgagaaagcg ggagacgagc tcctcgaaga tatcattcaa gtgcaaggtg aagcgcatcc
    57541 cgagccccgg ccgccggtgg tgacgatcat ggggcacgtc gaccacggga aaacgtcgct
    57601 gctggacgcc attcgtgaaa ccaaggtggc ggaaggcgag gccggcggga ttacgcagca
    57661 tatcggcgcc tacacggtca cggtccacgg aaaacaggtc acatttctcg atacgccagg
    57721 ccatgaagcg ttcacggcca tgcgtgcgcg tggagccaag gtcaccgata tcgtcatctt
    57781 ggtggtcgct gccgatgacg gcgtcatgcc gcagacggtg gaagcgattc atcacgcgaa
    57841 agcggcgggc gtcccgctga ttgtggcgat gaacaaaata gacaagccca ctgccaaccc
    57901 ggaccgtgta cgcaatgctc tttccgagca cgggttgatc tcagaggcct ggggcggcga
    57961 cacgatcatg gtcgaggtat cagccaaaca gaaaacaggc cttgatcagc tgctggaaat
    58021 gattctgttg caagccgaag tgttggagtt gaaggctgac ccgcatcaac ttgcaaaggg
    58081 caccgttgtt gaagccaagc tcgaacgggg acgtggacca gtcgccactg tccttgtaca
    58141 aagcggcacg ctacacgtgg gcgacgtgtt cgtcgtcgga gccttcagcg gcaaagtccg
    58201 tgcgctgatc aaccacaccg ggtccaaggt tcagcaggcc gggccctcgg ttccagtcga
    58261 ggtgattggt ttgccgggag tgccctccgc aggcgacgta tttcaagcgg tcaaggacga
    58321 acgcatcgct cgtgagatcg ccgaagatcg ggcccgcaaa caacgggccg cagagttggc
    58381 cggtgccgga aaagtgtcgc tcgatgatct cttcgcgaag atccaggaag ggtccgtcaa
    58441 ggagttgtcg ctcgtcatca aagcagacgt ccaagggtct tccgaagccc tggctaccgc
    58501 catcgaaaag ttgccgacag aggtggtgaa aatccgcgtg atccataacg gcgtaggcgg
    58561 gattaccgaa tccgatgtgc tcttggctgc ggcgtccaat gccatcatca tcggattcaa
    58621 catcaggccc gagcccaaag catcagatct tgcggagaag gaacatgtcg acgtccggct
    58681 ctacacgatt atttacaatg ccttgaacga tatcaaagcg gcgatggaag ggttgctgga
    58741 acccacgctc aaagagcgcg tgctcggccg ggcggaagtc cggcaggtgt tcaccgtgtc
    58801 caaggccggc acgattgccg gatcctacgt gatcgatgga acgattaccc gggcaagcgc
    58861 gggcgttcgg gtgattcgtg acaatgtggt ggtgtatcag ggaaggctgg gatcgctccg
    58921 tcgtttcaag gacgatgttc gcgaagtgca gcaaggctac gagtgcggta tcgctgttga
    58981 gaatttcagc gacatcaaaa gtggtgacgt catcgaggcc tacgctatcg ataagattgc
    59041 ggcaaaactg tgaccagaat cgtgaaaagt tctgccggct gtgtgccccc gatgtcggga
    59101 ggcgtagcgc ttcatacgat ttatgctccg ccgcacaggc gtgacatttt ggacctaccg
    59161 tccgccagtg tgctgtcggc agtcctgaca cgatagaccg tgagcgtcgt tgtcggactg
    59221 tgtacaatcg aactctttct gggcgacagt caatcgctga aagataagcg gcagattctt
    59281 tcgagtctca aagatcgact gcgacagaaa ttcaatctgt cggtggctga aatcgacgct
    59341 caagatctgt ggcaaaaggg agtgctcgca ttggcgtgtg tggcgaacga cggccgctat
    59401 gtgaaccagg tgctggacca ggcgcagaac atgatcaaaa gcgatccgat catcgagatc
    59461 gtccagtctc gaatcgagtt gttgtgaggg cggtcggtgc ctgtcaaagc gacaatggac
    59521 cggccataag gagctgggcg atggccaaaa caggatacag ccgggccgag cgcgtcgccg
    59581 atcaaattcg catggaggta gccgatatcc ttatgcgaag aattaaggat ccgcgcgtcc
    59641 gctcagtgac cgtgaccgat gtggagctca ccaatgatct gcggatcgcc cgagtcttcg
    59701 tgacagcgct ggggacggag gcggaaacga aggatgtctt cgccggtctg agcaaagcca
    59761 gcgggttcat tcggagcgag ttgggtagac ggctgacctt gcgatacttg cctgaagtgg
    59821 tctttcacaa ggatgtcagc ggccctcggg gagatcgtgt gttgcgattg ttggatgaaa
    59881 ttcacgacaa gggtcatggc cagtcagacg aacatgacga cgctccttcc agtcatcacg
    59941 gataacgcaa tgaccgctac agctcgagag tgtgatgtga tggacggcgt gctggtcgtg
    60001 cataaggagg ccggctggac gtctcacgac gtggtggcaa aagtcaggca tctgttgggg
    60061 gctaccaagg ttggtcacgc cggcacactc gatcctgccg caacgggcgt actgcctgta
    60121 ttgatcggaa agggcacgcg cattgctgag tatctcatgc cctgggacaa agaataccgc
    60181 gcgtgtttac ggctcggcga aacgaccgat acgcaggatg ccaccggaac ggtgctgcaa
    60241 cggcaaatga cgagcgatgt gacgccggat gatgtcgagg aagccatagg gcgttttaaa
    60301 ggtgaaattc aacaaattcc gccgatgtat tcagcgctga aagtccgcgg cgtgccctta
    60361 taccggtctg cccgtgccgg caagacgatt acccgagaac ccaggacggt gacgattcat
    60421 gaattggaag tactcacgat cgaagggtgt gacgtcagcc ttcgtgtggc ctgctcgaag
    60481 ggaacttaca tccgtacgct ctgcgccgat atcggggccg cactgggagt cgggggtcat
    60541 ttgcgcgcct tggagcgaac ccgcgtcggg ccgctgacag tggaccgggc cttgaccatg
    60601 gatgtcgtca tcgcccgcca cgcgctcggt cggctggcag acgagatgtg gtcgctcgat
    60661 gagattctag gagcgctgcc agcgttcgtc gtggaccacc agaccgcaga tcgggtgtgc
    60721 catggcgtcc ccgttgccta ctcgagtatt gtgtcgcccc gagcggaaga ggtccggagg
    60781 attggcagga cgcccgttcg tatccatgat caaggcggcc ggttattggc ggttgggagg
    60841 gcacctcaga cccaggttga tcggattgca attgagaaag tgctcgtccc tcagtcagga
    60901 tagtggacat aacaaggaga gaactcacat ggcgttgtta aaggcagcga aagcggaatt
    60961 gatcaaaggg taccggcagc atgacaccga ttccggatct ccggaggtac aaattgccgt
    61021 gttgacgaac cgcatcagtt atctgacgga ccatttcaag ctccataaga aggatcatca
    61081 ttcgcggcgc ggattgctgc agcttgtcgg ccgccgccgg agattgttgg actatctccg
    61141 agacatcgat gatgcgcggt atcgggcggt cattgagcga ttgggtattc gcaagtagtc
    61201 ggaggccctg cggtcacgag aaacagcagg caaccgtcgc acaggtgaac actgacatgc
    61261 gctcccatgc acgctgtgat gcggaggctg agtgcgccat tggcgatcat tcaagcctat
    61321 cgcacgtgac gaaggagcgc atgtctgtgg gcatctgtgg ggcataacaa gaggagacca
    61381 tagatggcac acgcagttga actcgatctg gcggggcgca cgctaaggct ggagaccggc
    61441 cgggtcgcca aacaggcgga cggctcgatc tgggcatcgt acggagatac agtggtcctc
    61501 gcgactgccg tcgcgtcaca gaacgccaaa cccggcgtgg attttctccc gctgaccgtc
    61561 gattatcaag aaaaggccta cgccgccgga aaaatccccg ggggttactt caaacgggaa
    61621 ggccgtccct ccgaaaagga agtgctcacc agccgattga tcgaccgtcc ccttcgcccg
    61681 ctttttcctg aaggctacta cttcgaaacc caagtcatcg cgtcagtgct gtcgtccgat
    61741 cggacaggct cttccgacgt catcggcatt accgccgcgt cagcggctct ggccgtatcc
    61801 aatattccct tcctcggtcc catcgccggt gtcaaaatcg gccgggtcaa cggtcaattc
    61861 atcgtcaatc ccgatctcga agcgttgaag ggaagcgaat tggatctcgt cgtggccggg
    61921 acggcggatg ccgtgatgat ggtcgaagcg ggggccaatg agttgccgga agcgacgatg
    61981 ctggctgcga tcgagttggc tcacagtgag atcaaaaaga ttgtcgcgaa gatcaacgag
    62041 ctgcgggtgc tatcgggtaa taagccgaaa cggatgatcg ctacggagga gattccttcc
    62101 gacttgacgg gtcaagtcaa ggcgctggtt gctcaaggaa ttcgtgaggc gatcatgatt
    62161 cccaataaga cggcccgaca ggagcgcctc gatctcatcc tgagagacgc catagaaaaa
    62221 ctgaagcaga cggacgatcc gaatcgtgaa cgccatgtga agatcgtgtt tcacggcctc
    62281 gaatacacag aagtccggaa catgatcctc gaaaaaggct cacgagcaga cggccggggg
    62341 ccggcggata tccgtcccat taccagtgaa gtcggcgtgc tgccgcgcac gcatggatcg
    62401 gccctgttta cccgaggcga aacgcaaagc ctggcggttg tgaccctggg cactactgat
    62461 gacgagcaac ggattgacgc gttggagggg gagtacacac ggacgttcat gctgcattac
    62521 aactttccgc ccttcagtgt cggcgaggcc agaccgctcc gctcgccagg ccgccgagaa
    62581 gtcggtcacg gggcgctggc agaacgggcg ctcaagcccg tcattcccaa caaggatcag
    62641 tttccataca cgttgcgcat cgtgtcggat attctcgagt caaatggttc gtcgtcgatg
    62701 gcgacggttt gcggaggcac actggccatg atggatgcgg gggtgccgat caaggagccg
    62761 gtagcgggaa tagccatggg gctgatcaag gaagacgatc gcgtcatgat cttatccgat
    62821 atcttagggc ttgaggatca cctcggcgac atggacttca aggtgtgtgg aacgaaacgc
    62881 ggcgtgaccg cccttcaaat ggacatcaag atcggtggca tcaccccggc actgatgcat
    62941 caggcgttgg aacaggcgaa ggccggccgc ttgcacatcc tcagctgcat gcagaaagct
    63001 ctggaggcgc cgcgtaccaa tatgtcggcg tttgcgccgc gcatctacac catgaaggta
    63061 aagcaggaca aaattcgcga agtcattggt cccggcggga aaactatccg cggcatccag
    63121 gctgactgtg gagtcaagat caacatcgag gatagcggca tcgtcactat tgcatcagtg
    63181 gacggcgcgt cgctcgagaa agccaaagag atggtcaacc ggatcgtcga agaggtggaa
    63241 atcggcaaga cgtacttggg cacggtgcga aagattatgg atttcggggc gttcgtcgaa
    63301 gtcctgccgg gaaccgacgg gttggtgcac atctcccaat tagcccacca ccgggtcaaa
    63361 tcggtggcgg acgaggtggc ggaaggtgat cagattctgg tgaaggtgct ggaaatcgat
    63421 cgccagggca aaatccgcct cagtcgtaag gaaaccatgc cggccccaac cggggccgga
    63481 accaaagacc aaacgtccgg gtaagcgtct tgtaccgcaa gctcgtactc gacaacggca
    63541 tgcgggtggt gaccgagcgg attccgacgc tcaagtccgt gaccgtgggc atctgggtca
    63601 acaccggctc gcgcgatgag caacctgccc aagccggcta ctcccatttc atcgaacata
    63661 tgttcttcaa agggacgcgc tcgcgctctg ccagcgagat ctcacgggag atcgacggct
    63721 tggggggcga gatgaatgca ttcacgacgc gcgagacgac gacgttttac gtcaaggtgc
    63781 ttgatcagca gcttcaacaa gcgctcgaat tgttggccga tctctttcat cactcccgtt
    63841 tcgattcgaa agaagtcgaa aaagagaagc aggtcgtctt ggaagaaatt cggatggtcc
    63901 aggacgaccc cgaagatttg gtgcaagaac tgcataccgg ccaggtattg gggagacatc
    63961 cgctcggtcg atcgattctt ggtcgcgagg atactattca gagcctcaag cgaccggacc
    64021 tgctgggcta tatcgacgca cattacgatc ccagccagat agtcattgcc attgccggca
    64081 gctttgaaca ggcgacggtt gaacggctcg tccagcgata ttttggaaaa cggaaaccga
    64141 ttcatggttc gttgagagcc ccgcgccgcc ccccggatgt gcgtgggggc gtcctcgcca
    64201 agaagaaaca acttgaacag gtgcatctgt gtctcgggct caaaggcgtc gccgccggcc
    64261 atcgtgaccg ctatgccgtc tatgccttga acagtgtctt gggcggaagc atgagttcgc
    64321 gtttgtttca agaggtacga gaaaagcgag gcttggcgta ctcgatctat tcctttctgt
    64381 ccggctattc ggacgtcgga accatcaccg tgtatgcggc gaccaggccc agagaagtgg
    64441 agcgggtcat agatctcgtg tgtagagaga tcacacgtat atacaagaat ggcatcgatc
    64501 gcaaggaact cgagcgcgcg aaaaaccaaa tgaaaggcag cctcatgctc agcctggaaa
    64561 gttcacacag ccgaatgagc aagctggcaa aggacgaact gatctatggc agacgcacct
    64621 cgctggaaga aatgctggcg catatcgatc gagtcacttc cgagcaagtc tctaccgcta
    64681 gccaacaact cttcgtgctt gaccacctgg cgatcacggc gttaggcccc ttgtctgctc
    64741 gcgccttgca ggggtacaca tgagaatttt ctaatagatc ggtcgaattg accgttcgcc
    64801 ttcgcccgtc ttgctgacag gaagactgtt gtaagtcatt gtatgattta cgcaaatcat
    64861 ctaaatccag gcagatatcc acaggacaaa catttccttg actcgtttcc gtgattttca
    64921 gtacgatggc cgcgttttta cttgaagact cttccatcga cgacgtgcgg cgtgagagcc
    64981 aaacaaaagg aggtggcaaa gtcccgctct cctgactgct gaatcgtcac aatcatgttt
    65041 tcgcattcat tgtcttacaa aaaagcgcgt tctcactcat gtgtggttat tttttatgaa
    65101 ggagggattt gagatgaaaa gggtcgtact tctagccgca gttgcagtgt gctctgtgct
    65161 cggcttgctg acggcagagt ttgccgccgc cgcggatgcg ccgggcggtc cgtcgggccc
    65221 gaccgacatt gttgtcggag ggaagccgct caagggtgaa gtcacgaaga ccctcaaggg
    65281 gcgcgtgtgg tcccaatggg ccgacaatcc ggacggcgag ctgctcttcg gcatacaata
    65341 ttggaatacc ggggagcccg cttccgggac tcccgcaggc cgttcatcca ccgatctcga
    65401 tgtgaagccg cccgatccgt tgttcgtctg ctgcgcgtgg ggatttgtcg gcggaacgga
    65461 cagaaacaat ccgtacgccg gttggtatca cgcggcgacc acggttcgtc ttgcagtaaa
    65521 ggacaaggcg ttgatggacc agattatcaa ggcatctcag gaattggtcg caatggaagt
    65581 gagcctggat ggccggacca tcacggggtt taaagtcatt aagtaatcga cttcgacaag
    65641 atatcattct ttcatgctgt tgaaggagga tgcgattatg aagtttcata tgaaaggctg
    65701 ggcccttgcg gccgctgttg cgctggtggt ttccatagcc tccaccgcca tggccattga
    65761 atcgtttcag gaacgttttg aatggggcga catgagcaaa cccacgaccc tgcaaggtcg
    65821 tgtcatcgtg ctcgatccct atgacgaggc ggtgtggatc aacatctccg tcttcggagg
    65881 taatgcggag agcggcttgt tctggcaaaa ggtgcatccg gggaagaacg taaaattcta
    65941 tgcggacaag ggcgtgtggg aagaactgaa gaagatgggt cggccccatg ctggcccggc
    66001 tgccgcgaag gaagtgcctc cgggcagcac gacggatttg atcgagttca ctgcggtcga
    66061 gacggagcag aatcaccggg tgattcagtc cgtgaagaag ctcccggaaa tcgccggtgc
    66121 ccctggcaag accatgagta tctcggcgct tcgattgaaa gactgtgccg gcaagaatga
    66181 agccgatgcc ggttgcgctg cggccaagca agggctgaat acctcgccca agggtgaagg
    66241 cgcggttcca atgcagtatg acgtattgac tccggacaag aagccgctcg gcggcctgat
    66301 tccttggacg gcaaagtaca ataccggtaa gtaagtcgga tccgaagcgc gtacgagaag
    66361 gcggtggctc gaaagggctg ccgccttttt atttgcgctg gggcgactcg gttgcgcgtt
    66421 tttgaagttc agctaaacgg ttgagcgcgt cgagcggggt caaggaaaac aggtcgatct
    66481 gcctcacttc atcgatgagc ggatgtggtt gaggaagtac agagcaagtc gggctctcga
    66541 cgacaacccg acgacattcg cgctcgcgat ccggttgttc caactgttcc agcacttcct
    66601 gcgcacgggc gatgacggga gtagggagtc ccgccagttt ggccacatgg atgccgtagc
    66661 tccgatctgc tccccccggc agaatcttcc gcaggaacac gacatctcca tcacgttctt
    66721 gcaccgcgac tcggtaattg cggattccct cgcgcaaggt ttccagctgc gtcatttcat
    66781 ggtaatgcgt agcaaacagc gttcgtgccc ctagatgctc tcgcgcgtgg atgaactcgg
    66841 cgattgccca ggcaatgctc aagccgtcgt aggtgctcgt cccacgccct atctcatcca
    66901 agagaatcag gctctgccgg ctggcgcagt tgaggatctg cgcggtttcc agcatttcga
    66961 ccatgaacgt gctccggccg gcggcgagat tgtcggaagc accgactctg gtgaagattc
    67021 tgtcaacaag gccgatctgc gcttcggcgg ctggaacgaa gctgccaatc tgcgcgagca
    67081 ggacaatcag cgcgacttgc cgcaaatagg tgcttttccc agccatatta ggtccggtca
    67141 gaatgaccaa tcggttgttg tcttgatcga ggaccgtatc gttggggacg aacgttcgat
    67201 cgatcgagag ttgttcgact accggatggc gaccattttt aatacggagc acccccgact
    67261 cgtcgatcgc cggccggacg taccgttgaa gggctgcggt ctcagcgagg ccggctagga
    67321 cgtcgatcag tgccagggat tgtgccatgg cttgcagccg aactccctct tgtgcgatcc
    67381 gttgccgcac ctgaatgaag gtttcttcct ccagcgccaa cagcttcgtc tcggcccccg
    67441 tcactcgttc ctccaattcc ttgaggccgg ctgtcatgaa ccgctctgca ttgactaatg
    67501 tctgcttgcg tatgaaatcg gatggcacct tggaaagatt ggtcttggtc acctcgatgt
    67561 agtagccgaa tacgtggtta aagcggatct tcaaagagtc aatgcctgtc cgctcgcgtt
    67621 cctttacctc caaagcagcg atccagccct tgccttccat atgggctttc cgcagttcgt
    67681 cgacaccagg gtgatagccg gaacggatga tgccgccgtc tcgtatcgag agtggagcat
    67741 ccggatcaat ggcctgttcg atgaggtcat agagatcgcg acagtcgtcc cattgatcgc
    67801 gccgttcgac gaggagggtg gatcgacagc actcaagctg ctgacggacg gccggaagag
    67861 cgcccaagga gtgttttaag gcgagcaatt ctcggggccc cgccacgtcg agcgtcactc
    67921 gtgtgctcaa gcgtgcaatg tcctgtacgg ttcgtagcgc cgcacggatg gccacacgct
    67981 cgctgatccg ttctttgagt tcatcaactc catccaaccg tgtgtcaatc gcatggcagt
    68041 cggtcagcgg tctcaagagc cagtcgcgaa gcagccggct tcccatcgcc gtagtggttc
    68101 gatccaggac ggagaatagt gtcgtgttac gaccgctgct cggctcctct ccaagtgagc
    68161 gcaccaactc cagattgcga atcgtcgcgc cgtccagatg catggcatcg cccctcttac
    68221 gacgcaggag gcgccgaaca tgcgctggag aagcagtggg ctgcgtctcg cggaagtagc
    68281 gccagacagc acctgctgcg cccagacccg cggtggtgtc atggcatccg aatccctcga
    68341 gcgaatgaac gccgaaatgg ctttgcagca ctcgggacgc ttcctcgtga tcgaaccagg
    68401 cttggtcccg ggcacagagt cgcgtcccgt gtagttgcga gagcgactgt gtcacgaccg
    68461 tgtccgattc cgagtgaagc agttctctcg gttggaggcg agccagctca tcggcgacct
    68521 gttgctcgtc gccctcccat tccatcagcc agaaatcccc cgtggtcaca tccaacgcgg
    68581 ccaagcccat acgcgtggct cccccggagg ctcgccgtgg cagccgggtg acagctgcga
    68641 ggaaactcga ctcggcggga tccaaaaatt ccgtatccac cagagtgccg ggtgtataga
    68701 ggcgcacgac ctcccgacgc acgagacctt tcgccgcctt gggatcttcc acctgctcgc
    68761 agagagcgac ggtgcgcccg gccttcagta atcttgcaat gtagccttga gcggcgtgat
    68821 aaggaacgcc gcagagaggg acaggatcgg cgctggactt gtctcgcgat gtcagggcga
    68881 tggacaggag tttcgaagcc tcctgagcat cctcgtagaa catttcataa aagtccccga
    68941 cgcggaacaa cagaatgctt tcaggatagc cgcgtttgat ctcgcggtac tgccgcatca
    69001 ggggggtcgt ttcctcctca ctcatcgacg ccttcccgtt gctgcaaatc cccgggcacg
    69061 ggcggtgcgg ctcggcccgt gagtttttcc accgagtgcc gaagtcgttc caggtccacc
    69121 tcggcttcct ggagcgcctt ggtttttcga cgcagttcga tgcgaccttt cacccaagga
    69181 ggaaacagaa ggatgaggga taccaacagg ccgattccga accccgctaa gatcggcttg
    69241 tagatcggcg tggaggcctc aaacaatccg aaaaaatagc gcagcgtgac ttcttgttcc
    69301 tgattttgaa gaaaaaaggc caggcacaac aagagcagcg ttccaacgag gatcacgcga
    69361 atcatcggga ggataatcct cttgcgctgg ccgaacgggg tcgtccggag gggagacgtc
    69421 tggatacacg cttcgttctc gtgcgggcca gcagggcagc cgacagcggt ccggtagcca
    69481 tgagtctgat acttctactc tgaaccgttc gatggcgcaa ctggatttca agacggtcgg
    69541 gggggagaat tccgggagcc ttgtcggccc attcgatcac gacaaccgtc gatccggaga
    69601 aataatcgtc gagtccgatt gaatccacct ctttgctcga attgagtcga tacagatcaa
    69661 cgtgcgcaag cggcaatctt ccacggtact cgtggagcag cacgaatgta gggctgctca
    69721 cggcgccgga tgtcgcgccg aggccggcag caattcctcg gacaagcgct gttttcccgg
    69781 atcccagtgg tccgtagagc gccagtgatt cgcctccatg cagcgctgaa ccaatcgcgc
    69841 gtcccaggcg atcggtgtga cgcggcgaag gtaaggccag ttgccatacg cgctcggaag
    69901 ttccgggcgt tcgattactc gctgatggct cggaatgcat acggaatgtg ttcgatcacg
    69961 tcgccggcaa tcataccagc cgatcccaag tgctcggcgg caagatcgcc tgccttgccg
    70021 tgcagatagg tcgctacgca ggccgcttcc caagacggga caccttgagc cagaaagccg
    70081 acaagcatgc cagtcaacac atctcccgtt ccagcggtgg ccatgccggg attgcccgtt
    70141 ggacagattg ccagaaggcc atccggacgc gcaatgacgg ttcgcgcacc cttcaatacc
    70201 aagaacactc cgcgttcgcg ggcaaaacgt gctgcagtgc ccaggcggtc ggcattcacg
    70261 ctttgtgaag tggcctccgg ctccaaccgg gccatttcac cagggtgtgg agtgagaacg
    70321 ggagggattt tgcacccggt cagcaatgac gcacgactcg ccagcgcatt caacgcgtcg
    70381 gcatccagca ccgaaggccg gtcaaggtgt ttcatcaagg actggacgag ctcaacggtc
    70441 tcaggatgag tcgagagtcc cgggccaata gcgatcgccg tacgggcttg catgaaggcg
    70501 agtattcgat cgacgccggc ccgactgagg gtgcgagctt ttgtctcggc aagaggctgc
    70561 gtcatggctt ccagcagttt tgcctccaaa acgtcattga cactggccgg cacgccgacg
    70621 gtgactaagc cggttccaac gcgaagcgcc gcccttgcgg cgagcgctgc cgcgcctgtt
    70681 ttcccgactg agcctgcgag gatacctgca tggccaaatg tgcccttgtg cgacgacgcg
    70741 ggacgtgggg gaagcgcctt cctgaccatt tcttttgtca cgagcactgt ccggctgtcg
    70801 atctcgtcca caaatgccgc aggaatgcca atatcgacga catgaatcga gcccgcatgg
    70861 tcgatgccct cacccaggta gaggccatac ttcggtaatc cgaatgatac ggtcgccgtc
    70921 gcacgaacgg cctgccccaa gacagcgccc gtgtctgcag caagaccgga cgggatatcg
    70981 acggcaatga cgggtttccc ggctcggttt atcatctgga tggcctcgcc atagactccg
    71041 gtcacggcag tggagaggcc tgtgccaagc aacgcatcca caatgacgtc acttgtcctg
    71101 aggatcgatt ccacctgctc gggcgaccgg aaggttttag tcgcggaagg tccggcgatg
    71161 cgccgccagc gttgaaacat cgccgcggca tctcgactca actgtgtcgc cgccgacagg
    71221 cacaatactg tgacgtccgc gcgatactga cgtaaccggc gggcagcgac gaagccatcc
    71281 ccgccgttgt tgcccttccc gcagacgaca gtaacgcgct tcccgcgaag gggtccaaac
    71341 cggctttgaa tgaaatcggc caatccaatt cccgcgcgtt ccatgaggat gagactcggc
    71401 acgcgcgctt ctagaatggt tcggcgatcc aaggcctgca tctggatccc ggtgacgatt
    71461 ttcatggggt ggctcaagcc aacattgagg agagtccgac cgtcaggaga gtacgtctcg
    71521 tcgttcagcc gagcagtgcc ttcatctcac gcacggcctg atcaaggccg acgaggacag
    71581 ctcgagcaat aatactgtgg ccgatattgt attccacgat ctcagggatg tgggtcaagc
    71641 gcttcacatt ctgataattc aacccatggc cggcgttgac ccctattccc agcttgtagg
    71701 caagcttggc ggcctgggtg atggcttcga attccgcatc tgcctctttc gagcgcttgg
    71761 cattggcata tcgacctgta tgcaactcta caaaatcagc ggcgacctta tgcgctgttt
    71821 tgatttggcc gaggtccggc tcgacgaaca gactgacggg aatgccgccg tcgtgcatca
    71881 ttgtgatgat ttgctgcacg cgttccttat gggtcagcac gtccagtccg ccttccgtcg
    71941 tcaattcttg ccgcttttct ggcacgagag tcacgagatc gggcctcagg gtgagggcaa
    72001 tcttggccat tgtttcatcg gcagccattt ccatatcgag ttttgtcctg atgatttccc
    72061 gcagcaggtg aagatcacgg tcctgaatat ggcggcggtc ttctctcaaa tgaacgacga
    72121 caccgtcggc gccggctaac tccacaagca cggctgcggc cagcggatcg ggatcttgcc
    72181 cgccgcgggc ttgccgcagg gtggccacgt gatcaatgtt cacaccgagt cgggccacaa
    72241 ccctcctgtt gaagtcatct ggtgaggagc gtcacaggca gaacaatgtg aatggtgcgg
    72301 atgccgatgt tgtaacagaa agccgcctag aggggcaagg ctgcgtgacc gcaaagggag
    72361 caactctcct ggccgtgacc ctcgcgagat tgccgtgcgg aaccccacag aatcacaaga
    72421 taatttgact cagcaacttc ctcgcaatta caataggcct ccatcttaac gtcagagcgg
    72481 tttgtcctcc ccggtgagcc gttcgccagc agcagccatg ggattgtcag ataattttta
    72541 ccggctcaag cggctcccgc cctacgtgtt cgcccaggtg caaagcctga agttggaagc
    72601 gcggcatcgt ggcgaggaca tcatcgactt cggaatgggt aatcccgacc aacccacgcc
    72661 gacccacatc gtggacaaaa tgatcgaggc cgcgaggaaa ggcaagaatc accgctattc
    72721 ggcctcacga ggcatcacaa aactgcgtca tgccattgct gcctggtatc agcgcaacta
    72781 tgatgtcgat ctcgacccgg agacggaagc gatcgtgacg atcggatcca aagaaggtct
    72841 cgcccatttg tcgctggcca tgatcggtcc aggggacgtt gtgctgacgc cgacaccgac
    72901 ctatcccatc cacatgtaca gcttcgtgat tgcgggagga gaggtgcgtg gaattgaatt
    72961 gaggccggat agtgattttt ttgacgacct ccaacgcgtc taccgccaga cgtttccccg
    73021 gccgaggatc ctcgtcatca actttccgca taatccgacg acggccatcg tggacctcga
    73081 attcttcaag aagatcgtcg cgtttgccaa agaacaccac gtcattgtca ttcacgacct
    73141 ggcctatgcg gatcttgtgt tcgacggcta tcgggcgcca agctttctgc aagtacctgg
    73201 cgccaaagag gtgggcgtcg aattttatac gctgtcaaaa gcctacaata tgccgggatg
    73261 gcgggtcgga ttttgtgtcg gcaatcggga ggtcgtaggg gccctggcga aaattaagag
    73321 ttatttagac tatggtattt tccagccgct gcagattgcc agcgtgattg ccttgaacgg
    73381 gcctcaggac tgtgtgaagg acacggttca acgctatcaa aagcgccgtg atgttctcgt
    73441 tggaggacta aaccgcatcg gctggaacgt caacaagccg ctcgcgacca tgtttgtgtg
    73501 ggcgaggatt cccctgccgt atcggcatat ggggtcgctc gaattttcca agctgctgct
    73561 gcgagaggcc aaagtcgcag tctcccccgg catcggcttc ggagaaggcg gcgatgaata
    73621 cgtccgcttc gcgctggtcg aaaatgagca ccggacaagg caagccgttc gtgggatcag
    73681 gaaggcattg aagctcgaag gaggcgaggc gtgatctcgc gggtgggcgt tggcatcgtc
    73741 ggctttggca ccgtgggctc gggcgtggcc aaaatactcc tggagaacgc agccctcatc
    73801 cgccgccgcg tcggcgtgcc gatcgagctg gttcgaatcg ccgatctgga tacgacccgg
    73861 gaccgtggca tggccctgcc ggccgggctc ttggtgaagg acgcgaagca gatactggcc
    73921 gacccgacca tcgacattgt agtcgaatta gtgggaggat acgatttcgc caaacgcctc
    73981 attcttgagg ccatcgacgc cgggaaaagc gtggtgaccg caaataaggc cctcctggca
    74041 ctccacgggg aagaaatatt tgcggccgcc tcccggagag ggatcgacat cggatttgag
    74101 gccagcgtcg gaggcggaat tcccgtcatt catgcgctca aggaagggct ggcggccaac
    74161 tcgatccaat ccatttatgg catcatcaac ggcacgtcga attatattct ctcccgcatg
    74221 acgaacgagg gaaaggggtt tgccgaaacg ttggtggagg cgcagaaagc gggatatgcg
    74281 gaggccgatc cagcattcga tgtggcgggc accgacagcg cacacaaact ggcgattctg
    74341 gtcaatctgg ggttcggcac gccggtcaac ttcaaggatc tttacaccga aggcattacc
    74401 catatcaccg agtacgacat cgcttacgcg cgcgaattcg gctatacgat caagctgttg
    74461 gggattgcaa agctcattga cggtgaggtt gaggcgcgcg tccatccgac catgttgggt
    74521 gaggagtcac cgttgaccaa aattgaaggc gtgtacaacg ccattcaatt ggtgggcgac
    74581 gcggtcggcg acgtggtttt gagcggacgg ggagcgggag atctggctgc aggaagcgca
    74641 gtggtcagcg atatcatcgc gggtgcccgc aatcttctca agggcgccgc aggtcgtgtg
    74701 ccggcggcat cctttcaacc gaatgagcgg cggccccttc gtatgcggcg aatggaggaa
    74761 atacgctccc tgtactactt gcgtttcatg gtcaccgacc gaccgggcgt attggcacag
    74821 atcgcaggtg aactggggcg atatggcatc agcattgcct cgatgttgca gaagggacgc
    74881 cgggagggac agaccgttcc catcgtcatc aagacccata cggcgacaga gcgtgacgtt
    74941 cagaccgcac tcagtcacat caatcggctc tcgttcatgt ccgagcccac cactctgatc
    75001 cgcatcgagg gcaaggacga ataagtcatg actcgctggc gcggcatcat tgcagaatac
    75061 cgaaaatttc ttcccgtcaa cgatcagact cccgtcatca cgctcgatga aggtaatacg
    75121 cccttgatcc aggcggccag gctcgccaag aagatcgccc caggaatcga cctgtatttg
    75181 aaatttgagg gagcgaatcc gaccggatcg tttaaggaca gaggcatgac gctggcgatt
    75241 tcaaaggccg ttgagaatgg cgcccgcgcc gtgatttgtg cttccacagg caacacgtca
    75301 gcgtcagcgg cggcctatgg tgcccgggcc ggcttggcgg tgtatgtgct gatccctgcc
    75361 gggaagatcg ccatgggaaa actctcgcaa gccatgatgc accaggcgac cgtcatccaa
    75421 gtcgacggaa actttgatca ggccctctcg atcgtaaaaa ccctctccgc cgcacatcac
    75481 atcgagctgg taaattctct caatccattt cgcatcgagg gacaaaagac cgcagccatg
    75541 gaggtgtgcg atcaattggg cgatgccccg gcggtccatg tccttcctgt cggaaatgcc
    75601 ggcaatatca ctgcctactg gaaaggctat aaggaatatc gtgcagccaa tcaaagccgg
    75661 ggcgtaccac ggatgatggg ttttcaggca gccggggccg cgcccatcgt gctcggccgg
    75721 atcgttgaga atcctcaaac ggtggcgact gcaatccgga tcggcaaccc cgccagctgg
    75781 caagcggcgg ttgacgcggt gaaggagtca tccggagcca ttgattccgt gaccgacgaa
    75841 gaaatccttc aagcctatgt tgcggtagcc ggtacggaag gagtgttttg cgaacccgcg
    75901 tcggcggcct ccgtggccgg tgtgatgaaa ctgagccgtc aaggtcggct gcgcgagggc
    75961 gaaacggtcg tctgcaccct cacgggtcat ggcttgaaag acgccgacac agccatcagc
    76021 gtatcggtca aaccgcaaac cgtcaacgca acaacggagg aagtggctcg acttctgaag
    76081 gtataggcac gatgcgagtg tgtggtcgat gaaatatgtg attctgcatg ccgagggaat
    76141 gtccgaccat ccccggcagg aactggccgg caaaactccg cttcaagccg cctccactcc
    76201 ccaccttgat cgattggccg agcagagcga actcgggctc ctcacggtcg ctctggacaa
    76261 tggccgtcat gggagcgggc tgacggggac ctccatcctc gggtatgagc ccaaaaagta
    76321 ctatcagggg ccgggaccgt tggaagcggc tagcttaggg gtcacagtag gcgaacacga
    76381 tgtggtctac cgctgtacga tggtgacctt gaaagcggaa ggtccgtcgg gcggcaaggc
    76441 aggcacccca gatatcaaga agcttggccc tcacatcgtc atggatgacg cgacagcagg
    76501 gctgatcacg agcgaggagg cccgcgagct gattgacgcc gtcaatgagc agcttggctc
    76561 agagatgatc caatttttcc ccggagcagg gcatcgacac ttgatggtat gggtcaatgg
    76621 gaagcctcgt gccgtctgcg tagatccgca atccttggtg ggccgtccga tcggagactc
    76681 tctgccggca ggcgatggag ccgacatctt gcggaaactg atggaggcct cgttgatgat
    76741 cttgcgcgat caccccgtga acgatgaacg acgggaggcg ggccataagc cggcgaattg
    76801 cctgtggctg tggggggaag gccgagccat cccgcggccc agcctgtctg aacgtttcgg
    76861 cgtgtccgga gtggtcgttt cttccggcga cgtccatcgc gggctcggtt tgctgtctgg
    76921 gctcgaggtg gtcgaccctg atcgtttgac caaccttcgg actcatgcgt cggtggcgct
    76981 tgatgaattg aagtccaagg acttcgcata cgtgcacatc gagttgcctg atcaggtcgt
    77041 ttatgatccg gacgtcaagg ccaaggtgcg ggcgattgag gaagttgata ccgaatttgt
    77101 cggccccttc ctctccggca tgaagacctt gggtccctat cggttggtga cggtcagtga
    77161 cccgggcagt gtgcaccagg gaaacatggc gcaagcgcac tggccgtatg cgtatcagga
    77221 ccgtacggca aaaccgtccg gttccggccg acggtttacc gaggtcgatg cgaaggcgat
    77281 gacgaccgct ccgcgggatg cgacgaagtt cgtcgtgcgg ctcttcgcca aaggctcatg
    77341 acgtggcgct gatcgttcag aagtatggag gaacctctgt cggatccatt gagcgcattc
    77401 accgcgtcgc ggatcgtgtc gagcaggcgg tcaaggaggg caacaaggtc atcgtcgtgc
    77461 tctcggccat gagtggagag accgatcggt tgatgaaact cgctcacgac gtggcggcgc
    77521 tcccggatga acgtgaactg gacatgttac tctccacggg cgagcgcgtg accatcgcgc
    77581 tcttggcgat ggtgttgcgc ggacgcgggc tggacgcccg ttcgtttacg ggacgccaag
    77641 tcggcatcat gaccgacagt gcccacacca gggcgcgaat cacgagggtg gctgctgagc
    77701 ggattcgcga cgccctgacc cgtggagtca ttcccgtggt ggcaggtttt caaggaatca
    77761 acgagcactc agacgtcacg acgttgggcc gcggcgggtc ggatttgacg gcggtcgcgc
    77821 ttgccggagc gttgaaggcc gatcgatgcg tgatctttac ggatgtggac ggcgtctata
    77881 cggcggaccc gaatatcgtt ccctccgcca gacgaatcaa tacgattgcg tatgaagaaa
    77941 tgctggagat ggccagtctc ggcgcgaaag tcctgcaaag ccgctcagtc gagtttgcgg
    78001 ccaaatacaa cgtaccgctt gaggtcaact ccagtttcaa ggaaggaaag gggacgcttg
    78061 tgaaacgcga agacgcagat atggaggccg ttgctgtctc cggcatcacc ggcgatcgca
    78121 accaggcgaa aatcacgctg gtcggggtgc ccgataggcc cggtatcgct gcgcggctct
    78181 tgggcccgat cgccaaggcg aacatcttgg tcgacatgat catccagaac gtaagccatg
    78241 aatccctgac cgacttttcg ttcacgattc ctcgcgtcga cgtcaagaag gccatgccga
    78301 ttatcgaaga gacggcgaaa gacatcggcg ccaagtcggt ctccgtgacc gaagccatcg
    78361 ccaaggtttc actggtcggc gtgggtatgc gctcgcattc aggcgtggcg gccaaaatgt
    78421 tcgatgtgct gtcgcatgaa ggggtcaaca tcatgatgat cagcacgtcc gagatcaaga
    78481 tctcctgcgt gatcgacgag aagtacatgg aattggcaat gcgtgcgctg catgccgcct
    78541 tcggactgga ccaaactccc gtcggcaaat aacgctccac agaccctcga caggcccgac
    78601 ccgctcctgg taccctcttc gccatggcca aacacgtacc gcattccgtt cgaaaatctt
    78661 cggtaccgag aaaaccggcc acgaaggcgg tttccgcgcc attggaaatc tacgatacca
    78721 cgctacgcga cggagcccag gctgaagacg tcacattttc tgccgacgac aaagtgatgg
    78781 tcgcccaacg gttggacgat ctcggcgtcc acttcatcga gggcggctgg cccggcgcca
    78841 atccgaagga cattgaattt ttccgcatga tcaaagaaac gccgctcaaa caggccactg
    78901 tcgtggcgtt tggctccacg agaaaggcca gcaatcctgt acagaaagac gccaatattc
    78961 aggccctcct cgacgccgaa acgaaagtca tcaccctgtt tggaaaaagt tggtcgttcc
    79021 acgtgaccga tgccctgggc atcgctctcg ccgccaatct cgagatcatc agcgattcca
    79081 tcgcgtattt gcggtcaaag caccggcggg tgttttacga cgctgagcac ttctttgacg
    79141 gctataagac gaatccagac tatgccctgg aaaccatcac acgtgcggtg gcggccggtg
    79201 ccgagcgcgt gattctgtgc gatacgaacg gcggcaccat gccctgggag atcaaggaca
    79261 tttgtcgtgt ggtccagagc gagtgccggg ttccgttagg cattcatgcc cacaacgatt
    79321 gcgacatggc cgtggccaat tccctggtcg cgctcgaaag cggcgtcgta caagttcagg
    79381 gcacgatcaa cgggatcgga gaacgatgcg gcaatgcgaa tttgtgctcg atcattccca
    79441 atcttgaatt gaagatgaag cggcctgcgt tgggcgatcg gttacaccac ttgaaggatg
    79501 tctccggctt cgtgacggaa attgccaact tgatgcctaa caagcaccaa ccgtatgtcg
    79561 gcgattcggc ctttgcccac aaggggggcg tccatattca tgcggtgctc aaaaatcctg
    79621 ccacgtacga acacgtggtc ccgtctctcg tcggcaatcg gcagcgcgtg ctggtatcag
    79681 acaacgcggg tcgcagcggg ctggtggaaa aagtggaggc ttacgggatc aagctggata
    79741 agaacgactc gaaggcccag gaactcatgg ctaccctcaa agagcgtgaa aacgaagggt
    79801 atcagtttga gggagccgaa ggctcttttg aactgctgat gcgcaaagcc atgggagcgc
    79861 acaagccgtc cttccagttg ctcgggttca gggtcattgt tgagaaaaaa caagatcttg
    79921 ggcactcagg ttcagaagcg accgtgatgg taaaggtcgg agatgtgact gagttggccg
    79981 cggcggctgg cgccggccct gtcaatgcct tggaccacgc gcttcgaaag gccttggaga
    80041 aattctatcc gcaactccga gaggtcagat tg
//
LOCUS       DASSCQ010000125         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_180564, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000125 DASSCQ010000000
VERSION     DASSCQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180564"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..558
                     /locus_tag="VFH05_08860"
     CDS             136..558
                     /locus_tag="VFH05_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678367.1"
                     /translation="MPDNISPKVKALASRLMALEAAAAKRAGVHPSSAFRLCERLREP
                     LSRLAGIAGFRSLLSRALALATDEVRWLKAIHVAADGSLEGLDEAEAQQPEDQIAVGE
                     IVLVAQLLALLVTFIGQELTLRLVEEAWPRATFDDLND"
     gene            574..2118
                     /locus_tag="VFH05_08865"
     CDS             574..2118
                     /locus_tag="VFH05_08865"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET6678368.1"
                     /translation="MARKIKTTGRQRQETSTAKPGSVVIRQLPTGIHGLDAILGGGLP
                     EFSFNIIAGGPGCGKTTMAHQFMFANATPKRPALYFTILGESALKMLRYQQQYTFFDP
                     AKLNGAIHFINLSQIVLEQDLSTVLETIIKEVETTHPGIVVVDSFRTALRKKPDVTEM
                     DVQFFVQHLALHLTTWEATTFLIGEYVEAEIQENPVFTVADGLVWLSQVAERNSVVRK
                     LQVLKLRGQASVPGLHTFRITRDGLQTFSRTLGLSERKARPDHRPRLSIGIRELDTML
                     GGGIPFGDSLLVAGSSGTGKSVFASHFIAEGLRRGERGIMAVFEERPEDVKRRAKNLG
                     MDLAKAERNGTLKILYLRPLDLSIDESLQAILDAIKEIDAKRLVIDSLVGFEMALAPG
                     FRTDFRESLYRMMASVTGAGVTVLSTVELDENFTEFRFSHYMVSFLADDIIRLRYVDI
                     DGVLRRILTIGKMRDSQHSKDIREYEITKKGVALTETRLNQYRALITGIPAYWKPPGR
                     EGDPRK"
     gene            2115..>3773
                     /locus_tag="VFH05_08870"
     CDS             2115..>3773
                     /locus_tag="VFH05_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678369.1"
                     /translation="MKPRKKPNPLAANLPELCHLLMENAPVPMAECEGAGHIIRYVNP
                     AFCRLVGTDREALTGKPFAETTQEGDRCLAVLNRVFRTAECVSYTDSEQGESHPPYWS
                     YAIWPVLGPNQRPVGVMMQVTETTSFHQQAVAMNRELLLSSLRQHELTEVTTRTNDEL
                     EQRVLERTRALVASEEALRALATELNLAEQRERKRLATELHDYLAQMLVLGSMILGQA
                     KRTGLPPKGEDFVKQTEEVLGNALTYCRTLMAELSPPILQEQGLPAGLTWLADHMKRH
                     ELAVAVEIQDTSDMPLPIDRAVLLFQSVRELLINVAKHGTVKEATVKLSYKSGMLQII
                     VNDTNGFDLAAGSHDISPLSSKFGLFSIKERMKALGGSFELHSAVGQGTTATLTLPLA
                     SSSVAEGATYQTENSTTRVAKVLPLATVPSPSRERPPLRQQNAPIRVLLVDDHTVLRE
                     GLRSIVSAYQHLEVVGEASDGREAVNLARNLDPDIVVMDINMPHMDGIEATKQIKSYQ
                     PDIAVIGLSVNPSAVTEQKMKAAGASAYLTKESAADALYQAIQEA"
BASE COUNT          867 a         1052 c         1079 g          775 t
ORIGIN      
        1 ttccccgcgc cttgtgcccc agcagcttcc acaactgctc gcgtaacaaa cgcgggcagg
       61 cttcccgcgc gcatccgaag cccatatgca aaaggaatta cccacgtagg tgctgcgtgc
      121 agccaaggtt gcgacatgcc agataacatc tctcccaagg taaaagcttt ggccagccgg
      181 ctcatggcat tagaggcggc tgcagccaaa cgcgccggtg ttcacccatc atcggccttt
      241 cgcctctgtg aacgactgcg tgagcctctt tccagactcg cgggaattgc cggttttcga
      301 tcgctcttgt cccgcgctct ggccctcgcc actgatgaag ttcgctggtt aaaggccatc
      361 cacgttgcag cggatggctc cttggagggg ttagacgagg cggaggcgca gcaacccgag
      421 gaccagatcg cagttggcga gatcgtgctc gttgctcaac tgctcgctct gctggtgacg
      481 ttcataggac aagaactgac gctgcgcttg gtggaagaag cgtggccacg tgcaacgttc
      541 gatgacctga atgactaaac ctcaaggagc atcatggcga ggaaaattaa gacgacggga
      601 cgccaacggc aggagacatc caccgccaaa ccaggcagcg tggtcatccg ccaattgcct
      661 accgggatcc acgggctcga tgcgatctta ggcggcggcc tgccggaatt ttctttcaat
      721 atcattgctg ggggacccgg ctgcggaaaa acgacgatgg cacatcagtt catgtttgcc
      781 aatgccacgc cgaaacgtcc ggcgctgtac ttcaccatcc tcggcgagtc cgccctcaag
      841 atgttgcggt atcagcagca atacaccttc ttcgatcccg ccaaattaaa cggcgcgatc
      901 cactttatca acctcagtca gatcgttctg gagcaggatc tcagcacggt gctggagacg
      961 atcatcaagg aagtggagac gacccatccg ggcattgtgg tggtcgattc ttttcggacg
     1021 gcgctgcgga aaaaacctga cgtaacggaa atggatgtcc aattcttcgt gcaacacttg
     1081 gccctgcacc tgaccacttg ggaggccacg acctttctga tcggcgaata tgtcgaggcc
     1141 gaaatccagg aaaatccggt ctttaccgta gccgatggct tagtctggct ctcgcaagtg
     1201 gcggaacgca attcagtggt acgcaagctg caggtcttga aactgcgagg ccaggcctcc
     1261 gtgccgggcc tgcacacgtt tcggatcacg agagatggcc tccaaacctt ctcgcgcacg
     1321 ctaggactga gcgaacgaaa agcgcgtccc gaccatcgtc cgcgcctgtc gatcggcatt
     1381 cgcgaactgg acactatgct cggcggtggc atcccattcg gggatagtct cttggtggcg
     1441 gggtcttccg ggacaggcaa atcggttttc gcctcgcatt tcattgccga aggtctccgg
     1501 cgcggcgagc ggggcattat ggcggtcttc gaagaacggc ccgaagatgt gaagcggcga
     1561 gcgaagaacc tggggatgga cctagcaaag gcagaacgca atggaacatt gaaaatcctc
     1621 tatctgcgcc cgctggatct ctcgattgat gaaagcctgc aagcgatcct cgatgcgatc
     1681 aaagagatcg atgcgaaacg tctggtgatc gattcactcg tcggtttcga gatggcgctc
     1741 gcgcccggct tccgcaccga ctttcgcgaa tcgctttacc gtatgatggc ctcggtgacc
     1801 ggcgcggggg tgacggtgct gagcacggtg gaattggatg agaactttac ggaatttcgc
     1861 ttcagccact acatggtgtc gtttctggcc gacgatatta tccggctgcg gtatgtggat
     1921 atcgacggtg tgctgcgccg catcctcacg atcgggaaaa tgcgcgacag ccaacacagc
     1981 aaagacattc gcgaatatga gattacgaaa aagggcgtgg cactgaccga gacccgcttg
     2041 aaccagtacc gcgcgctcat caccggcatt ccggcgtact ggaaacctcc tggccgagag
     2101 ggagacccga ggaaatgaaa cctcgaaaaa agcccaaccc gctggcagcc aatctgccag
     2161 aactctgcca tttgctcatg gaaaatgctc cggtgccgat ggccgaatgt gagggcgctg
     2221 ggcatatcat ccgctatgtc aatcccgcct tttgccgcct ggtcggcaca gatcgcgagg
     2281 cactgacggg aaaaccattt gcggaaacca ctcaggaagg cgatcgctgc ctggccgtct
     2341 taaaccgcgt gtttcgcacg gccgaatgtg tcagctatac cgactcggaa caaggtgaat
     2401 cgcatccacc ttattggtct tatgccatct ggccagttct gggacctaat caacggccag
     2461 tgggagtcat gatgcaagtc accgagacca cttcgttcca tcagcaggcg gtggccatga
     2521 accgcgaatt actgctttcg tcattgcgcc agcatgaact cacggaggtc accacaagaa
     2581 ccaacgatga actagagcag cgtgtcttgg aacggacacg agcactcgtc gcctcggaag
     2641 aggcgcttcg ggcattggcc accgagttga atctcgcgga gcagcgggag cggaagcgtt
     2701 tggcgacaga gctgcacgac tatctcgccc agatgttggt gcttggtagc atgatactcg
     2761 gtcaagcgaa gcggactggc ctgccgccga agggggaaga cttcgttaaa cagaccgaag
     2821 aggtgttggg caacgccttg acctattgtc ggacattgat ggccgaactc agtccaccaa
     2881 tcttacagga gcagggactt ccggctggtc ttacatggct ggctgatcat atgaagcggc
     2941 acgaattggc ggtcgcggtt gagattcaag acacgagcga catgccgttg ccgattgacc
     3001 gcgccgtcct cctgtttcag tctgtccgag aattactgat caatgtggca aaacatggga
     3061 cggtgaaaga agccacggtg aaattgagct ataagtctgg aatgctgcag atcatcgtga
     3121 atgataccaa tggctttgac cttgccgcag ggtcccatga tatttcaccg ttgtcctcca
     3181 agttcggcct ctttagcatc aaggaacgga tgaaagcgct tggagggagt tttgaactcc
     3241 actcggcagt aggacaggga actaccgcaa cgcttacgct ccccttagct tcatctagcg
     3301 tggccgaggg tgctacttat cagacggaaa actcaacgac acgagtggct aaggtgctcc
     3361 ctcttgcaac cgtcccttca ccatctcgtg agcgtccacc tttgcggcaa cagaacgcgc
     3421 cgattcgcgt tcttcttgtg gatgatcaca ccgtgctgcg cgaagggtta cggagcatcg
     3481 tatcggcgta tcaacattta gaggttgtag gggaagcgag cgatgggaga gaagccgtca
     3541 accttgctcg gaacctcgat cctgacatcg tcgtgatgga catcaatatg cctcatatgg
     3601 atgggattga agcaacgaaa cagatcaaga gctaccagcc ggacatcgct gtcatcgggc
     3661 tgtcggtcaa tccatcggcc gtaacggagc agaagatgaa agcagccggc gcatccgcct
     3721 acctgaccaa ggaaagcgct gcggatgcct tatatcaggc catccaagaa gca
//
LOCUS       DASSCQ010000126         5932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1117292, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000126 DASSCQ010000000
VERSION     DASSCQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117292"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4239)
                     /locus_tag="VFH05_08875"
     CDS             complement(<1..4239)
                     /locus_tag="VFH05_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678370.1"
                     /translation="MTNHPELEEPIRAELFGVERLEQHAESLATAQIITGDSRRGRLL
                     TPRVLENGRVLVKCYRTIALAIREEKAITPAAEWLVDNFHIVDEQLREIIDDLPPGYY
                     RQLPKLASGHLENYPRVFGVAWAFVAHTDSRFDPEMLRRFVAAYQRVQPLTIGELWAV
                     AISLRVVLVENLRRLAERIVHSRAARLEADELADGLLGTGVLDVETSATALRRFEHRP
                     LATAFAVQLVQRLRDLDPKVRPVLLWLDERLAAQGTTANDIVHAEHQQQAAMSVTVRN
                     IITSMRLTSAFDWADFFERVSLVDDILHREPRYTEMDFVTRDTYRHAIEDLSRGSGFS
                     EIEVTTRVVDRARRAVTEPHESEQMERHSDPGYYLISQGRRAFERELGYRVSWKHRFL
                     RWYVQASAPGYLGSLAVVTAIVMALTLWRSVEAVETTAGLVLLGLLALVPASDLAIAL
                     INRTVMAILGPRPLPRMALRNGIPEELRTMVAIPTLLTNQQGIEEQVERLEVHYLANP
                     DDDLRFALLSDWRDAPSESIPGDAELLATAVEGIARLNKRYGPASGGGSRFVLFHRRR
                     VWNRSEHTWMGWERKRGKLHELNQLLRGSTHTTFISVGGQPPERIPSVRYVITLDADT
                     RLPRGAAHRLIGTMAHPLNRPRFDPLVGRVVEGYGVVQPRITPSLPTDGEGSCFQRTF
                     SGPSGIDPYASAVSDVYQDLFREGSYTGKGIYEIDAFEAALADKVPDNAMLSHDLFEG
                     LFARTALATDVELFEAFPSHYEAAAARQHRWARGDWQLLPWLLGRGHTGSEPHRAVMI
                     PAIGRWKILDNLRRTLSAPAAFLALLAGWLLPQGAAWVWSSFILATIAIPSLLSFVLG
                     LYPRRRGISIRSHVRGAVTDLKLAATQIALTVTFLAYQAWLMSDAILRTLSRLVITRT
                     HLLEWVTAAQAEDAYTCNLWGMYRRMAGGIVLTVMAACGVALFGWNDSWAAAAPFILL
                     WMAAPAVARWVSLPPRFTGAEPVTPADARTLRLIARRTWRFFETFVSPEDHALPPDNF
                     QEDPKPVVAHRTSPTNIGLYLLSTLVARDLGWLGTLEASERLEATLATMSGLELFRGH
                     FYNWYDTRTLHPLDPKYVSSVDSGNLAGHLVVLSNGCRELIQQSSVDVNLFAGIDDAI
                     RLLREALAEIPDQRQTHTVTRKQLSQAVEALAMLLLSRPLDAIGWTTRFGRLTALSNS
                     VADMAQALAQERSDGPEGELRIWADSVRKCVESHLRDVELLFPWVRLSAKDLASMFDS
                     PSGPVLEWETIEPFFHSVPTLAGAPERFAAALRALDRLREDSLSHPLQNRASLARIAL
                     LADAIRQSSADATALIRRLAAIAQTAEQMVRAMDFTFLFDPTRKLLSIGYRVAENSLD
                     PNCYDLLASEARLASFIAIAKGDVPASHWFHLGRA"
BASE COUNT         1345 a         1692 c         1748 g         1147 t
ORIGIN      
        1 ggcgcggccc aggtgaaacc agtgcgatgc cggcacgtcg cctttggcga tggcgataaa
       61 gctggccagc ctggcctccg acgcaagcaa gtcgtagcag tttggatcca agctgttttc
      121 ggctacgcga tagccgatgg acaacagctt gcgtgtcgga tcgaagagga aggtgaaatc
      181 catggcgcgc accatctgct ccgcagtctg cgcgatggct gcgagtcggc ggatgagtgc
      241 agtggcatcg gcggacgatt gccgtatggc atcagccagc agagctatcc ttgccaggct
      301 ggccctgttc tgcaacggat ggctgagcga gtcttcacgc aaacggtcga gcgcacggag
      361 cgcagccgcg aaacgttcag gggcgcccgc cagcgtggga acggaatgaa agaacggctc
      421 gatggtctcc cactccagta ccggcccgga cggactgtcg aacatggacg caagatcttt
      481 cgcactgagg cggacccacg gaaacaacag ttccacgtcc cggagatgac tctctacgca
      541 cttcctcacc gaatccgccc aaatgcgcaa ctcgccctca ggcccgtccg agcgctcctg
      601 tgccagggcc tgcgccatat ctgccacgga attggacaat gcggtcagtc ggccaaaccg
      661 cgtggtccag ccgatggcat cgagcggccg cgacagcagc aacatggcta gcgcttcgac
      721 ggcttggctc agctgtttcc gtgtgacggt gtgcgtctgt cgctggtcgg gaatctcggc
      781 gagcgcctca cgtaacaacc ggatggcatc gtctattcca gcaaacagat tgacgtcgac
      841 agaagattgt tgaatcaact cacggcatcc gttgctcaag accacgagat gtcccgcgag
      901 attgccgcta tccacggagg acacatactt cggatcgagc ggatggaggg tgcgtgtgtc
      961 gtaccagttg tagaagtgcc cgcgaaatag ctcgaggccg ctcatggtcg ccagcgtggc
     1021 ctccaggcgt tcgctcgctt ccagagtgcc caaccaacca agatcacggg caaccagagt
     1081 cgagagcaaa tagagcccta tattggttgg agacgtccgg tgggccacga cgggcttcgg
     1141 atcttcctga aagttatccg gtggcagcgc atggtcttcc ggtgacacaa atgtctcgaa
     1201 gaacctccag gtccggcgcg caatcaaccg gagcgtccgg gcatcggcgg gcgtaaccgg
     1261 ctcagcgccg gtgaaccgcg gaggcaggct gacccagcgc gccaccgccg gagccgccat
     1321 ccataggagg ataaacgggg ccgctgcagc ccaggagtcg ttccatccaa acagtgcgac
     1381 tccgcaagca gccatgactg taaggacgat accgccagcc atgcgcctgt acatgcccca
     1441 caggttgcat gtgtaggcat cctcagcctg tgccgcggtt acccactcca acaagtgcgt
     1501 ccgtgtgatg acgagccgac tgagggtccg aagaatcgca tccgacataa gccaggcctg
     1561 gtatgccagg aaagtgaccg tcaacgcgat ttgagtcgcg gctaacttca gatcggtaac
     1621 tgcaccacga acgtgactgc gtatggaaat accgcgccga cgtggataga gtccgaggac
     1681 aaaagataag agcgatggaa tcgcgatcgt ggccagaata aaggaggacc agacccaggc
     1741 ggccccctgt ggtaacagcc acccggctaa caaggccaga aatgcggcgg gagccgacag
     1801 ggtacgacgg aggttgtcga gaatcttcca acgaccgatt gcgggaatca ttactgcgcg
     1861 atgcggctca gatccagtat ggccgcgtcc gagaagccaa ggcaacaact gccaatcgcc
     1921 gcgtgcccac cgatgttgcc tggctgccgc ggcttcgtaa tgggaaggaa aggcttcgaa
     1981 cagctcaacg tcggtcgcga gcgccgtacg agcgaagagc ccttcaaaga gatcgtggct
     2041 gagcattgca ttgtccggta ctttgtccgc caaggcggcc tcgaaggcgt cgatctcgta
     2101 aatgcctttg ccggtataag acccttcacg gaacagatcc tggtacacat ccgacacggc
     2161 ggatgcatag ggatcaatcc cgctggggcc ggaaaacgtc cgttgaaaac aagagccctc
     2221 accatctgtc ggtagcgagg gcgtaattcg tggctgcacg acgccgtacc cttcaacgac
     2281 gcgtccgacc agagggtcga accgaggccg gttcagaggg tgggccatgg tcccgatcaa
     2341 gcggtgtgcg gctccgcgtg gcaaccgtgt atccgcatca agcgtgatga cgtaccgcac
     2401 agaaggaatg cgttccgggg gctgcccgcc gacggagatg aacgtcgtgt gtgtcgagcc
     2461 gcgcaacaac tgattcaact catgcagctt gcctcgcttc cgttcccacc ccatccaggt
     2521 gtgctcggac ctgttccaga cacgcctgcg gtgaaacagc acaaaacgtg aaccaccgcc
     2581 ggacgcaggg ccgtaccgct tattcaggcg tgcgatccct tcgactgccg tggcgagtag
     2641 ctccgcgtca cctggaatac tttcgctcgg ggcatctctc cagtctgaga gcagggcgaa
     2701 gcgcaggtca tcatccggat ttgccagata atggacttcc aatcgctcca cttgctcctc
     2761 gataccctgc tggttcgtga gcagggtggg aattgccacc atcgttcgca actcttcagg
     2821 aatgccattg cgcagtgcca ttcgaggcaa tggccgcggt ccgaggatgg ccatgaccgt
     2881 gcgattgatc agcgcgatgg cgagatccga ggccggcaca agcgcaagga gaccaaggag
     2941 cacaagaccc gctgttgtct cgacggcttc cacggatcgc cagagcgtca aggccatgac
     3001 tatggcggtc accactgcga gggatccgag atagcccggc gcggaagcct ggacatacca
     3061 acgcaggaat cggtgcttcc aggacactct gtagccgagt tcccgctcaa acgcccgtcg
     3121 tccttgcgag ataaggtaat agccagggtc cgaatgccgc tccatctgct cactctcatg
     3181 cggctccgtc accgcccgcc tggcgcgatc cacaacacgg gtcgtcacct cgatttctga
     3241 gaatcccgag ccacgggaca aatcctcgat ggcgtggcgg taggtatccc tggtgacaaa
     3301 atccatttcg gtgtatcgag gctcgcgatg aagaatgtca tccacgagac tgacgcgttc
     3361 gaaaaagtcc gcccagtcaa atgctgacgt cagtcgcatg ctggtgatga tgttccgcac
     3421 agtcacgctc atggccgcct gctgctggtg ctcggcgtgc acgatgtcgt tggcggtcgt
     3481 accctgcgcg gcaaggcgtt catcgagcca caggaggact gggcgcacct tgggatccag
     3541 atcacgtagt cgctggacaa gctgcacggc aaacgccgtt gccaacggtc tgtgttcaaa
     3601 gcgacggaga gcggttgccg aagtctctac gtccaatacc cccgtaccga gcagaccgtc
     3661 ggcgagttcg tcggcttcca gccgggccgc gcggctgtga acgatccgct ctgccagtct
     3721 gcgaaggttc tccaccagca cgacacgcag cgaaatggcc accgcccata attctccgat
     3781 cgtcagcggt tgcacgcgct gataggccgc cacaaaacgg cgcagcatct cggggtcgaa
     3841 tcgactgtcc gtgtgggcga cgaatgccca ggccactccg aagactcgtg gataattctc
     3901 cagatggccg gatgccagtt tgggcagttg cctgtaatac ccgggaggta aatcatcgat
     3961 gatctcgcga agctgctcgt ccacgatgtg aaagttgtcg accaaccatt cggcggcagg
     4021 ggtgatcgct ttttcttctc gaattgcgag ggcgatggtc cggtagcact tcaccaagac
     4081 ccgtccgttc tccaggactc gtggcgtgag taaccgaccc cgccgtgagt cgccggtaat
     4141 gatctgggca gtcgcgagac tttcagcatg ctgttcgagt ctctctacac cgaaaagctc
     4201 tgcccgaatc ggttcttcca gctctggatg attggtcata gatgaaccgc cctgattagg
     4261 aggaagtcac tgcgagatag atggcttgaa cgctattact gaaagcgcca gaccaataga
     4321 acttgccacc agccgtcatt aaaaaggtta gagcctaccg ctcacagtgc acaggactcc
     4381 gggtctacgc tcttctttat tccgagaaga tgtggcgtgg acatcgtgct tcgtcgaaca
     4441 gtagatggat ttggcaaccg tcttggtcag tatgtcgccc tgtacggcct ttcaagaggc
     4501 cgtcggcggt tggtatgtag aggggcagtg cgtactctag aagataccac agaagatacc
     4561 acccagatcc acatgccaga atcctcctcg tattgtcggt ggtaggcttg cttacagttg
     4621 ggacagttcc atagttcact caccccgtag ccgattggtg caacctgata gcagagacag
     4681 ccagcggtgt tcatgcccct ccattaacgc gcgatatgtg tgtggacgaa gacagacgca
     4741 ttctcgctga atagaggtct gagtaggtgg tcaaaactgc tcacgtgcgt cttgcatgtg
     4801 gttcgggctc cgtgaaagca aggggggcct cagaagcgga aacggacgta ggcgcgctta
     4861 tggcaccttc tgcactgcca tgggtaccat cccacccaac tgagcagatg atccgaccaa
     4921 tgccggcgag ccaggcgttg ccacgtgcac cgacattcgg gcgtgggcag tgcgcggctc
     4981 acggtttcgc gagttcgcgc ttgttggccg gtcacaggta atttttcttc caatagcata
     5041 gaagagacta cgacactgaa accatctgtt tagaatagtg aattctaaaa tgaggaggaa
     5101 cgggtaccga gcagaataat agtacgaggg aaacggctag ggtggtcaaa attgctcagg
     5161 cactggaaga agagaagggt acgattggcg tctcttcgtc aaaggcggcg gctgccatgg
     5221 ctgccaattg aagaatatct aggcgcagcc tggagccctc atctctgacg tagcttaccg
     5281 acggcgtctt accgcttctg aatcgaataa ggggaacggc ctatgagtga actagtctac
     5341 ggttactttc ttcacgtcaa cggacaaaga atgcaggaag tcttcgacca atttaaggac
     5401 gccattgaag cagccaggcc acatatggat atgacaaaga tggcagcact caaaattcaa
     5461 tccacaagtg acgtaccagc gaaaacttgg aattacgact atggactgag tacgtgggtt
     5521 cccctttggc aaagttaact aagcgcgcga ccagtaagcc agcggttttg cagcatggct
     5581 agtcgagatt ttaacgttta agggcgcata tctggagaat gggacgtgct tagagatcga
     5641 ggaggcgcga ggtcaagcag tcgcggtctg aagcaggcat ccgtaagggc attttacccc
     5701 tgaccatggc cgggtaaaga ctcaccccgt gcaacatttc cattgaggag gcaaacacca
     5761 tggcaacagt tcgagacact ttgcgatccc cgggcactcc tgatcagcaa tcgtttatcg
     5821 tgaggagaat actgggcacg cttaccgagc aaaaggagtg cagcttggaa gatttaatcg
     5881 agatttgtgg cgcaaccatc tcgaatcagg tcctcctcga ggtcgaccgg at
//
LOCUS       DASSCQ010000127        15853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1249910, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000127 DASSCQ010000000
VERSION     DASSCQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15853
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249910"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..788
                     /locus_tag="VFH05_08880"
     CDS             396..788
                     /locus_tag="VFH05_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678371.1"
                     /translation="MVEVGADNERFVLALPPGDYELIRIQITEGPFLSIAQLASGFSI
                     GPDPITYLGTWRFGVDSPKYGRMVSISMVNDEQDLADIVQTLALNDPSLDREVVTTVL
                     PDPPQLQARLFEVMPYPRVPRYFRRHWW"
     gene            917..1825
                     /locus_tag="VFH05_08885"
     CDS             917..1825
                     /locus_tag="VFH05_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET6678372.1"
                     /translation="MGTLVSITAVGQTQAEASAAITAGFEEVKRLELLLSTWIPGSEL
                     SRVNAAAGRSAVMVSAETMTVVRKSLQVAEITEGAFNIAIGPAVDAWNISAEPRLPTV
                     EELDALKPLVDLQYVHTDVWERSIHLQKPGMRIDVGGIGKGYAADQAVLVMKKAGAIA
                     GVVALSGDIKTFGQLAGGEAFPVRIRHPRKENEILAQVTLQEEAISTAGDYERFFEKD
                     GVRYHHILDPQTLQPARGCQSVSVIAKEGIWADGLDTGIFVLGVKRGMELVEQLENVE
                     AIIVDDVGRVHVSSGLRDRVVLMSDH"
     gene            1842..2147
                     /locus_tag="VFH05_08890"
     CDS             1842..2147
                     /locus_tag="VFH05_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678373.1"
                     /translation="MKRMWLLLFASTLVGALLACSDDGPYYKNKVNEVMMDHIAKRYG
                     SPHKVEELEGNQTRWTYFKRSSGTVGYSGIAREEACQAVALTFDRHNILRDWQQVKC"
     gene            complement(2169..3005)
                     /locus_tag="VFH05_08895"
     CDS             complement(2169..3005)
                     /locus_tag="VFH05_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET6678374.1"
                     /translation="MTADDLERNDFSIGWLASLGLHAALAFVALTVAQRVTFVPHIPF
                     TWQVAMVASSSNRADPSAALSGNPETSRQNLHARAPVPSPSSDTPQMPRTVRPTAPMA
                     TGSHIVPTSAPVEPAEKPIPQTIFKAETAAASRLDSRQAEWPSEPDSIRLPVPAQAIQ
                     QQSEGLSPAATPSEVAKVTSATRPDYTWLFETILRRMEELKKYPTEARLNQAQGKVVL
                     KAIIRSDGSVEDVEVAQSSGHQTLDEAAVELLKVAGPFELPRPLEKPQLTVKIPMSYR
                     LQ"
     gene            complement(3002..3376)
                     /locus_tag="VFH05_08900"
     CDS             complement(3002..3376)
                     /locus_tag="VFH05_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET6678375.1"
                     /translation="MDRELDQINVIPLVDVMLVLLVIVLTTATFITTGQIPVNLAKAK
                     EAGDRKDVPIVITLNAEGNLFVNDRPVTTDGLKETLRVEPRESAVVVRADKTTLLEKF
                     VSLVDDVRGLGFQHVSLEVVRL"
     gene            complement(3360..3791)
                     /gene="exbB"
                     /locus_tag="VFH05_08905"
     CDS             complement(3360..3791)
                     /gene="exbB"
                     /locus_tag="VFH05_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HET6678376.1"
                     /translation="MDALSNAVDYGIIGLLLVLSVWSVAIAVERFLYYRRIDLAQFHS
                     QQTLEIALTKRLVVIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGTMAVNTIMIGLSL
                     ALKATAMGLLVAIPCVVMNNILRRRVTELVAQYKVKHGSRA"
     gene            complement(3860..4990)
                     /locus_tag="VFH05_08910"
     CDS             complement(3860..4990)
                     /locus_tag="VFH05_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6678377.1"
                     /translation="MKPRQAITGESILQADLYQPVSSVLELRGVSCAYDAGRPAIENI
                     AFAAKEGEILCLLGPSGCGKTTILRAIAGFEPVRSGQIFLSGHLVSSPDHMTPTEDRH
                     VGMVFQEYALFPHLRVQDNIAFGLRHLSRHDRKIRVHDMLRLVGLEGFERRYPHELSG
                     GQQQRVALARALVQHPVVLLLDEPFSNLDPDMASRMRQELHDLLRRTKTTTILVTHDH
                     DEAFAMADRIAVLNQGKLEQFDTPEIIYHMPQTPFVADFVGQADFIAGTVTNGMVQTE
                     LGEFSNTLECEEGTEVVVMIRPDDIHLVPTKGATSHVLSRQFRGSENLYAVSLPSGQI
                     VHSSQGSTSVYEVGTPVELRVLASHSVIFKQTLSAPRATPAR"
     gene            complement(5008..6591)
                     /locus_tag="VFH05_08915"
     CDS             complement(5008..6591)
                     /locus_tag="VFH05_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET6678378.1"
                     /translation="MTTIRRQLTSPLQLTTLVTATLILLPLGYVMSQALSADPAVWSR
                     LWNTRIPELLTNTIRLAASVACFTLVLGVSAAWLVTRVEFPARRLLEGALVLPLAMPT
                     YVLAYIYSYLLGYGGPIEQLWQTVAGPQARVFSPHSFLGATLVMTLDTFPFVYLLSRS
                     ALLNMNVSFEEVSRASGISRMRTLSLVTLPLMRPSIVAGLALVVLYVVSDFGAVSLLR
                     YQTLTYAVFQQLTGRSDTTAASILSLLLVGMALVFLITERWFRHRSRFYQTTGRYRTP
                     ERHRYGWMGTSLALIYLVLVVAASFGVPAYLLITWSLSPEALATIDTRFYGFFGNTAF
                     LAACAATGGVVIGLPLAYMANRRPNWLNLGCLQAAYAGYVLPGPVAALAVLVLCLKLV
                     PFVYGSVIVLIVAYIIHFLPAALQSLEPALQQITPNLEEVARTLGFGVRETWQRVTLP
                     LMRSGFVVAWVLMFLQTMKELPASLLLRPVGFDTLAIRVWMEASEEYYQLAAPSALLI
                     VLLGLPTLLLLVSKDWRAA"
     gene            complement(6742..7758)
                     /locus_tag="VFH05_08920"
     CDS             complement(6742..7758)
                     /locus_tag="VFH05_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6678379.1"
                     /translation="MTIRIGLLALACLLATLLQPMSAPSAETLTVYSGRAERLIKPVL
                     DEFSAKTGVQVSLLSSGTTELVNRLKAEGDRTPADLFLTNDAGSLEQARVAGVLRPLN
                     MREVERAIPPQFRAPDNAWIGLSGRFWIIVYNKTLVKPGELKSLLDLGDPKWKDRIAI
                     PNAGSEYLQAGVSVIRATHGDAKTKQFLQGLKENAGGQVYQKSSQIVEAVAKGQASVG
                     IVNHYYVYRHLAAQPSAPIAVFMPDQQETGMGAIMNVAGVGIVKSTKHLETAKLLVEF
                     LVAQAGQKLFADLDKEYPLHPDVKADPALVERKTFRAAQVPLTRLAELREPTLTLIEQ
                     VGLR"
     gene            complement(7820..8257)
                     /locus_tag="VFH05_08925"
     CDS             complement(7820..8257)
                     /locus_tag="VFH05_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET6678380.1"
                     /translation="MGSGKFAKEMNALKEHLGKHQLKFTRQREMILTAFLRQEHITAE
                     AMYHQLAKTDPHLGLATIYRTLNLFCDAGIAQARHFGSQTQYDNVSHKGHHDHLICTG
                     CGKIVEFENCEIEKLQEEVAARNGFTIQTHRLELYGLCSRCRH"
     gene            8389..8952
                     /locus_tag="VFH05_08930"
     CDS             8389..8952
                     /locus_tag="VFH05_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678381.1"
                     /translation="MRTPHQITVDRALLLYVLHLAEPHGLLSDVKLQQLCFLCELQMF
                     GKGLKGFHFEFFRFAYGAFSKDLDNDLTSLRRKERVENFTLADQTQEQVLPLIEEGVA
                     GVDTNEKVMEILKAVMTTYGPQDSGTITSSVEAVELSTPQDPEFKIPIRDIVFHTTLL
                     VPQRIDVQAEFTFPPALLSRVNQAMGY"
     gene            complement(8974..10506)
                     /locus_tag="VFH05_08935"
     CDS             complement(8974..10506)
                     /locus_tag="VFH05_08935"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET6678382.1"
                     /translation="MKTLKTLEPLTRRLLEIQRVNSAASVLSWDQETYMPAGGGAARA
                     EQIATLQGIAHQKLVSLETERLLGGWLDLHTGLPLEQDGELWDESSRALLREVWRDYS
                     RSKKLPSDFVMQLSRECSLAQQVWAEAKAQNNFKLFLPSLRTVLSLKREEAQFLGFTG
                     SPYNALLDSYEPGSTVAALAPLFAQLKAHLVPLLTKIQQSRLQIDDSFLYDAYDQARQ
                     LEFGRLVLIAMGYDFEKGRLDLSAHPFTTSFHPTDVRVTTRVFEHDLPSCLFSCIHEG
                     GHGLYDQGLDPAHYGTPLGDSVSLGIHESQSRMWENCVGRSRAFWRFFYPILQATFHH
                     QLRAVNIDQFYTVINRVKPSLIRVEADELTYNLHIMLRFEIEQDLVEGNTNPDDLPAI
                     WNSKMKEYLGVSPSNDAEGVLQDVHWSMGGFGYFPTYTLGNLYAVQFYEQAKVEIPHL
                     EDEIAAGQLMVLRRWLAQKIHRWGRMFPPEHLSQRVTGVSLSPEPFLRYINRKYGDLY
                     HL"
     gene            10610..11260
                     /locus_tag="VFH05_08940"
     CDS             10610..11260
                     /locus_tag="VFH05_08940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6678383.1"
                     /translation="MGELVPAEIEAYAVEHSMEESELCRDLRAETHRHMESPQMLVGP
                     LEGALLKMLTQLVRATHVLEIGMFTGYSALCFAEALPHHGTVTTCEIDEASAALARRY
                     FARSPHGGKISIRMGPAIETMRTLERQYDVIFIDADKQNYVNYYLRGKELLAPEGVIL
                     IDNVLWDGDVLVMPPPDERTAAIQAVNRVVASDPEMGAVLLTVRDGVLLVKRKSVG"
     gene            complement(11286..13673)
                     /locus_tag="VFH05_08945"
     CDS             complement(11286..13673)
                     /locus_tag="VFH05_08945"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET6678384.1"
                     /translation="MIHRPRFDPPPHIYPPDEWNLIEKRYMPALLEQGETMFALGNGY
                     LGMRGCFEEGGPIGQNGTFINGFYESWPIVYPEDAYGLATAGQTIVNVTDSKIIKLYV
                     DDEPFWLPHGNARSFDRRLNMKSGTLDRDILWETPAGKQVSITSRRLVSFEHRHVAAI
                     FYEVTLLNAAAPVVLSSEIIANEPAPRRANYDPRQAKVFVEKVLNHRMSYAKEQRIVL
                     CHATNKSGLIVACAIDHRLETTSHVTWNTKYTPDAGQAVATIDAEPGCPITLVKYMVY
                     HTSQTAPPEEMCARAERTLDRAVAAGFPALLSGQEQYMNDFWQRSDVKVITNPARAKA
                     STTAVQQAIRFNLFHVLQASARAENTGVPAKGLTGAAYEGHYFWDSEIYVLPFLTYTA
                     PRIAKNLLRFRHGMLDKARERARQLNQRGAMYPWRTINGEEASAYYAAGTAQYHINAD
                     IMYALKKYIQATDEEEFLFDEGVEMLVETARLWRSLGYYSPRKGEQFSIHSVTGPDEY
                     NTVVDNNVYTNLMARENLLYAAETVERVKQRRPDLYTALVHKTRLEPSEVTEWKQAAD
                     RMYVPYDERLKINPQDDHFLEQEPWDFDRTPADKYPLLLYFHPLTIYRHQVIKQADLV
                     LAMFLLGHEFAPDLKRRNFAFYDPLTTGDSSLSACIQSIMAAEVDEMDKALEYAKAAT
                     LMDLGDVAGNVKDGCHIAAMGGVWMVLVYGFAGMRDYTGELCFWPSLPEVFNCIRFNL
                     IFKGQTLEVDVGHEVTHYTLKSGKGLTIRHEGQAIELSPGTPSVQQPNGRPSKDV"
     gene            complement(13698..14480)
                     /locus_tag="VFH05_08950"
     CDS             complement(13698..14480)
                     /locus_tag="VFH05_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HET6678385.1"
                     /translation="MRLNLKEFGAVLCDLDGVITQTARLHAAAWKRLFDDYLQEVVAR
                     TGAPFKPFDLEEDYRVYVDGKPRHEGVRDFLASRKVSLPPGTPSDGPDRETLYGLGQR
                     KDKHFKVALRETGVIAYPNTVKFLHLARAMGLKMAVVSSSHHCAEIIETVGLSSLFDV
                     RVDGHEIDRLQLAGKPAPDSFLEAARRLAIEPRRGIVIEDALAGVQAGHAGGFGLVIG
                     VDRHNQAQALREQGAEIVVADLGELLSPLMDTADSSTPSVHT"
     gene            14634..15539
                     /locus_tag="VFH05_08955"
     CDS             14634..15539
                     /locus_tag="VFH05_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6678386.1"
                     /translation="MAIQPAGFGYGAIVVAAVLWGGSIVAQKLALSGFSAVEASVLRD
                     IGGLIILMTTWWIQDGSLRGITRQDYRTLALLGLGVLGNHLLILIGLKYVSGAVAGVI
                     IGALPVVTALLSAVLSQDVPLRLVWAGSVLSFFGVALVSVAGFNAAGEQPLLGGGLVF
                     LGVASWALYSIGSRTIMERVPALTVNWTTLLAATVLQIPLLWTDQKMVRAGLASVTLL
                     DWLALAYLVVFATAVAQQAWLFGVKRIGPSRASVLGNLTPVSAVALSAVILKEPVGVV
                     EIIGIVLILAGVWVVHRQTSNLPSS"
     gene            15691..>15853
                     /locus_tag="VFH05_08960"
     CDS             15691..>15853
                     /locus_tag="VFH05_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6678387.1"
                     /translation="MLERRIEEVTKANQRFYEAFESLDIAKMDEIWAHLDYVTCVHPG
                     WTLRSDWPAV"
BASE COUNT         3446 a         4316 c         4602 g         3489 t
ORIGIN      
        1 ttcggacgtt gcgtgtgatg aataaggggg catcccgggg ttgcttctga tattgagaac
       61 aattattaaa atcgtcggca gtataagcga cgggcttttg actgtcaaga gggcactacc
      121 aacaggcttg gaaatgctac tgcccttgag gcggcaatgc cgtataatct cctgtgattg
      181 gaaggctgtc attcctccct tggtctagtt tggctttaac cctttttctg caggcgtgca
      241 caactggact cgctgccatc cctgaggagg gcacggtcca ttcgaacgtc gttgttggaa
      301 gagtgattgc ggtgatcaca ggagagcgga gcaggaaatt cgaaccggct gttaaatcgt
      361 ttgaagtgag gaaccgcgcg accaacgagc ggtttatggt tgaggtgggg gctgataatg
      421 agcgattcgt tctggcactg ccaccgggag actatgaatt aatccgcatt caaatcactg
      481 aagggccatt cttgtccatc gctcaactgg cctcagggtt ttcaatcggc ccagatccga
      541 tcacctattt gggaacctgg cgattcggag ttgactctcc aaaatacggc cgcatggtgt
      601 ccatttccat ggtaaatgac gaacaggacc ttgccgacat cgtccaaaca ctcgcactca
      661 acgatccatc acttgatagg gaagtcgtga ccactgtgct gcctgatcca ccccagctgc
      721 aagctcgact gttcgaagtc atgccatacc cgcgcgttcc gcgctatttc cgtcgtcact
      781 ggtggtgaaa accgtccgca tgcctttcaa cgtcgctatg tttcttatcg tttgcctatc
      841 gttcgcggcc gtgtcttgtg tcaaaggcgg ctctcccgga gcttcgccgg tcgcagtaaa
      901 gcgaacccag atgcatatgg gcacgttggt gtccatcacg gccgtcggcc agacacaagc
      961 cgaggccagc gcagccatca ctgccgggtt cgaggaagtg aagcgactag aactcctgct
     1021 cagtacctgg atcccgggaa gcgaactgtc ccgggtcaac gcggccgccg gccggagcgc
     1081 cgtgatggtc agtgcagaaa cgatgacggt ggtgaggaaa tcgttgcaag tggctgaaat
     1141 aaccgaaggg gctttcaaca tcgcaattgg tccggccgtt gatgcgtgga atatttcagc
     1201 ggaacctcgg ctcccgacgg ttgaagaatt agatgcgctg aagccgctgg tggatttaca
     1261 gtatgtacac acagacgtat gggagcggtc gattcatctt caaaaacctg gcatgcgcat
     1321 cgatgtagga ggtattggga aaggctacgc ggcagatcaa gctgtattgg ttatgaaaaa
     1381 agcaggagcc atcgcgggcg tcgtagcgct gtcaggagat atcaagacct ttggtcaatt
     1441 ggctggtggt gaagcatttc cggtcagaat cagacatcct agaaaggaaa acgaaatctt
     1501 ggcacaggtc accctgcaag aggaggccat ttcgaccgcc ggcgattacg aacggttttt
     1561 tgaaaaagac ggtgttcgat atcatcacat tctcgatccc caaacgttgc agccggctcg
     1621 aggttgccaa agcgtaagtg tgatcgctaa agaagggata tgggctgacg ggttggacac
     1681 cggcatcttc gtcttaggag tcaagcgggg gatggagtta gtcgagcagt tggagaatgt
     1741 tgaagccatc attgttgatg acgttggacg cgtgcacgtc tcatcgggcc tgcgagatcg
     1801 cgttgttctt atgtctgatc attaatcgag ggaaggacgc tatgaaaaga atgtggttgc
     1861 tgttgtttgc atcgacactc gttggggcat tgctcgcttg ttctgatgac gggccgtatt
     1921 acaagaacaa ggtcaacgag gtaatgatgg atcacatcgc taagcgctat ggttcacctc
     1981 acaaggttga agagcttgag ggaaaccaga ccagatggac ctacttcaag cgaagcagcg
     2041 gtaccgtcgg ctacagcggc attgcgagag aggaggcgtg tcaggccgtt gcattaacct
     2101 ttgacaggca caatatcttg cgtgattggc agcaggtcaa gtgctagcta aatgtggacc
     2161 gatccctgtt attggagacg ataactcatc ggaattttca ctgttaattg cggtttttcc
     2221 aggggtcggg ggagttcaaa agggccggcc actttcaaga gctcaactgc agcttcgtcg
     2281 agagtctgat gacctgagct ttgggcaacc tcaacgtcct cgacgctccc atcacttcga
     2341 atgattgctt tcaacaccac ctttccttgt gcctgattca atctcgcctc ggtcgggtat
     2401 ttcttgagtt cttccatccg acgcaggata gtttcgaaca accaggtgta gtctggacgg
     2461 gtagcggaag tcaccttcgc gacttcagat ggagtcgccg ccggcgagag gccttccgac
     2521 tgttgctgga tagcctgagc aggaaccggt aggcgaattg aatctggctc agaaggccat
     2581 tccgcctgtc gactatccag tcgcgatgca gcagccgttt ccgccttgaa gatggtctgc
     2641 gggattggtt tttccgctgg ctcaaccggc gcagaagtcg ggacgatgtg tgatccagtg
     2701 gccataggag cagtgggccg gacggtacgt ggcatttgcg gagtgtccga tgacgggctt
     2761 ggtacaggcg cccgggcgtg caggttctgc cgggatgttt cgggatttcc ggatagagcg
     2821 gcagacgggt ctgcgcgatt cgaagagctg gcgaccatcg ccacctgcca tgtgaacgga
     2881 atatgtggaa cgaatgtgac gcgctgggca acggttaatg cgacaaaggc cagggcggca
     2941 tggagaccga gcgatgccaa ccagccgata gagaagtcgt ttctttcaag atcatcggct
     3001 gtcatagccg gacgacctcg aggctaacgt gttggaagcc aagccctcgt acatcatcaa
     3061 cgagcgagac aaacttttcc aacaatgtgg tcttatccgc gcgaacaacg accgctgatt
     3121 cacgaggctc cactcgaaga gtctccttca atccgtctgt cgtcacaggt cggtcattga
     3181 caaacaaatt cccttcggca ttcagtgtga tcacaatggg cacatcttta cggtcgccgg
     3241 cttctttggc ttttgccaga ttgacgggaa tctggccggt agtgatgaac gtcgcggtcg
     3301 tcaacacgat gacgagcagg accagcatca cgtctacaag gggaatgacg ttgatttgat
     3361 caagctcgcg atccatgctt caccttgtac tgggccacca gctccgttac tcgccgacgc
     3421 aggatattat tcattacgac gcaggggatc gctacgagca ggcccatcgc ggtcgctttg
     3481 agcgccaggc ttaaaccgat catgatcgta ttgacggcca tggtgccaga cgtccccatg
     3541 gtgtgaaagg tgagcatgat gccgaggacc gtgccgagca aaccgatata cggcgcgttt
     3601 gcagccacgg tgccgatgac gacgagccgc ttggtgagtg cgatctccaa ggtctgctgg
     3661 gaatgaaact gggcgaggtc gatgcgccga tagtagagga accgttcaac tgctatcgca
     3721 accgaccaga cactcaacac cagcagcagg ccgatgatgc cgtagtcgac tgcattgctc
     3781 agggcatcca tgaagcgctc cttctccggg atcgttcttt cgatgaaagg gtgactgtgc
     3841 cagaagggga aaaccagtgt caacgtgcgg gtgttgcccg gggagcagag agagtctgct
     3901 tgaagattac ggaatgggaa gccaagacgc gcaattccac cggagtgcct acttcataca
     3961 cgctcgtgga accttgactg ctatggacga tctgcccgga cggcaggctg acagcataca
     4021 gattttcaga tcccctgaat tgccgcgaga gaacgtgtga tgtcgcgcct ttcgttggca
     4081 ctaagtggat atcgtcaggt ctgatcatta ccacgacttc cgtgccttct tcacattcga
     4141 gcgtattgga gaattccccc agctcggtct gcaccatgcc gttagtcacg gtgcccgcaa
     4201 tgaagtccgc ttgcccaacg aaatcggcta cgaacggcgt ttgcggcatg tgataaataa
     4261 tctccggcgt atcgaattgt tcaagtttgc cctgattgag cacggcaatg cggtcggcca
     4321 tggcaaacgc ttcatcatgg tcatgtgtga ctaatatcgt cgtggtcttc gttcgccgca
     4381 ggagatcgtg caactcctga cgcatgcggc tggccatatc tgggtccaaa ttgctgaagg
     4441 gttcgtcgag cagcaggacg acggggtgtt ggaccaacgc cctggcaagc gccacccgct
     4501 gctgctggcc gccggacaat tcgtgcgggt accggcgttc gaacccttcc aggccgacaa
     4561 ggcgcaacat gtcgtggaca cggatttttc gatcatgccg gctaaggtgg cgaagtccga
     4621 aagcaatgtt atcctgcacg cgcaggtgtg gaaacaacgc atactcctgg aagaccattc
     4681 cgacgtgccg gtcttcggtc ggtgtcatat gatcgggcga cgacaccagg tgcccggaca
     4741 ggaagatctg tcccgaccgt accggctcaa acccggcgat ggcacgcagg atggtggtct
     4801 tgccgcaacc tgacgggccc agtagacaga ggatctctcc ttctttggct gcgaatgcga
     4861 tgttctcaat ggcggggcgt ccggcgtcat aggcacaaga gacgccgcgg agctccagca
     4921 ccgaggagac cggctggtaa agatcggctt gcagaatgga ttcccctgtt atggcctgcc
     4981 gaggcttcac gtttgcagct tccttcttta ggccgcgcgc caatccttcg acactaatag
     5041 cagcaaggtc ggcagtccga ggagcacaat cagcagcgcc gacggcgccg ccaattgata
     5101 gtactcttca ctggcctcca tccacactcg aatggccagc gtgtcgaatc cgaccggccg
     5161 cagtaacaag gacgcgggca gctctttcat cgtttgcaaa aacatgagca cccacgccac
     5221 gacgaatccg ctgcgcatca gcggcaacgt tacgcgttgc cacgtctcgc gaacgccgaa
     5281 cccgagcgtg cgagcgactt cttccaggtt gggcgtgatt tgctgcagcg cgggttcaag
     5341 ggactgtagt gcggccggga ggaagtgaat gatgtatgcc acgatgagga cgatgaccga
     5401 gccgtacacg aaggggacga gcttcaagca caaaaccagg acggccagcg cggccaccgg
     5461 tccggggagc acatagccgg cataggccgc ttgcaagcaa ccgaggttca gccagttcgg
     5521 acggcggttt gccatatagg ctaatggcag accgatcact acgccacctg tcgccgcaca
     5581 agcggcgaga aaggcggtat tcccgaagaa gccgtagaag cgggtatcga tcgtagcgag
     5641 cgcttcgggc gaaaggctcc aggtgatcaa gagataggcc ggcactccaa aggatgcagc
     5701 gacgacgagc accaggtaga tcaaggccag ggatgttccc atccagccat atcgatggcg
     5761 ttcgggcgta cgataccgtc cggtggtttg gtagaagcgg ctgcgatggc ggaaccaccg
     5821 ttcggtgatc agaaacacga gcgccattcc taccagcaga aggctgagaa tgctggccgc
     5881 agtcgtgtct gaacggccgg tcaactgttg gaacaccgca taagtcaaag tctgatagcg
     5941 cagcaatgat accgcaccaa aatcggacac cacatagagc acaaccagtg cgagccccgc
     6001 gacgattgac gggcgcatca atggaagtgt caccagggac aacgttctca tacgcgaaat
     6061 gccactggca cgagatacct cttcgaatga gacattcatg ttgagcagcg cactccggct
     6121 cagcagatac acgaagggaa acgtatccag cgtcatcacg agtgtggcac ccagaaaact
     6181 gtggggggag aagacgcgcg cctgagggcc ggccacggtc tgccagagtt gctcgatcgg
     6241 cccgccgtag ccgaggagat acgaataaat atacgccagg acgtaagtcg gcatggccaa
     6301 tggcagcacg agcgcacctt ccaagagtcg gcgagcagga aattcgaccc gggtgacaag
     6361 ccaggcggcc gagaccccga gcaccaatgt aaagcaggct acgcttgcag ccagtcgtat
     6421 cgtgttggtc aacaactcag ggatacgtgt gttccatagc cggctccaga cggccggatc
     6481 ggccgacaag gcctgagaca tcacatagcc cagcggcagc agaatgagcg tcgcggtgac
     6541 cagcgtcgtg agttgcagcg gcgaggtcag ctggcggcgg atggtggtca caaatgactc
     6601 aactgggtgt gtgaaagacg acgattccac aggagtgttg ctgtatgagg tcgtgcctga
     6661 gcacgcgaca aaaaactggc caagcggggg tccgtgcacg ggagaatcgt cggtaagcag
     6721 caatgggtcc aggacattcg gttaccgaag gccgacttgt tcaatcaggg tcagcgttgg
     6781 ctctcgcaac tcagccagtc ttgtcagagg gacttgcgcg gcacggaaag tctttcgttc
     6841 cacgagggcc gggtcagctt tgacatcggg atgcaaggga tattccttgt ctaaatcggc
     6901 gaacaatttc tgaccagcct gtgcaacgag aaattcgaca agaagcttgg cggtctccag
     6961 atgtttggtt gacttcacga tgcccacacc tgcgacgttc atgatcgcgc ccatgcctgt
     7021 ttcctgttga tcgggcatga acacagcgat gggcgccgac ggctgtgccg cgaggtgacg
     7081 gtagacataa taatggttga cgatgccgac cgaggcctgc cctttggcga ctgcttccac
     7141 gatctgggaa cttttctggt agacctggcc tcctgcgttc tctttgagtc cctggagaaa
     7201 ttgcttcgtt tttgcgtcgc cgtgtgttgc ccggatgacg gatacgccgg cttgcaggta
     7261 ctcgctaccc gcgttaggaa tggcaatcct gtccttccac tttggatcgc cgaggtcgag
     7321 caaggacttc agctcacccg gtttcacgag cgtcttgtta tagacaataa tccaaaaccg
     7381 gcccgagaga ccgatccacg cgttatccgg cgctctgaat tggggaggta tcgcccgctc
     7441 gacttcgcgc atattcaagg ggcggagaac accggccacg cgggcctgtt caaggctccc
     7501 agcatcattg gtaagaaaca gatcggcggg cgtccgatcg ccctctgcct tgagccggtt
     7561 aaccagctcg gtcgttccgg aagagagcaa actcacttgt acgccggttt ttgcgctgaa
     7621 ctcgtccaac acaggtttaa tgagccgttc cgcgcgtccc gaatacacgg tgagtgtctc
     7681 cgctgagggt gcagacatcg gctggagaag cgtcgcgagg aggcatgcca atgcaaggag
     7741 acctatccgg atcgtcatga ggggccttaa ttgatattga taagctgtct caagacgata
     7801 tcaaaaagac ttgggatcgt caatgacggc agcgagaaca gaggccgtag agttccagcc
     7861 ggtgagtttg gatcgtgaag ccattgcgtg cggcgacttc ttcctgaagc ttttcgattt
     7921 cgcaattttc gaattcgacg atcttgccgc aacccgtgca gatcaggtgg tcatgatgac
     7981 ccttatgcga cacgttgtcg tactgtgtct gggagccaaa atggcgggcc tgcgcaatgc
     8041 cggcgtcgca aaacaagttc agggttcggt agatggtggc gagtcccaaa tgcgggtccg
     8101 tcttggcgag ttgatgatac atggcttcgg cggtaatgtg ttcctgccgt aaaaacgctg
     8161 tcagaatcat ttcccgctga cgcgtaaact tgagttgatg cttaccaaga tgctccttga
     8221 gcgcattcat ttcttttgca aatttgccgg accccatgcg gaacgcctcc gaccggtggt
     8281 cattaaaaac gaaaacgcgc tgtttggtca aggggcgtgc aattgacgcg gttttgcggg
     8341 gctgtttata gtgcagcgcc gctccctttt gtctaagagt gttaacagat gcgcacacca
     8401 caccagatca ccgtcgaccg ggcattgctc ctctatgtcc tgcatctcgc cgaacctcac
     8461 ggactgttga gcgacgtcaa gcttcaacaa ctgtgtttcc tgtgtgagct gcagatgttc
     8521 ggcaagggcc tgaaaggctt tcatttcgaa tttttccgct tcgcctacgg ggcgttcagt
     8581 aaagatctcg acaacgatct gacctcgctc agacgcaagg aacgagtcga gaacttcacg
     8641 cttgccgatc agacgcagga acaagtcctt ccccttatcg aggagggggt ggccggcgtt
     8701 gacaccaatg aaaaggtcat ggagattctc aaggcagtga tgacgaccta cggcccccag
     8761 gacagcggca caattacctc ctccgttgaa gcggtcgaac tcagcactcc ccaggatccg
     8821 gaatttaaaa ttcccattcg cgacattgtc tttcatacca cactgctcgt gcctcagcgc
     8881 atcgacgtgc aggcggaatt cacctttcct ccggctttgc tatcacgagt caatcaggcg
     8941 atgggatatt aggacggctc aagtcgggcc cggtcacagg tgatacaggt caccgtattt
     9001 ccgattgatg taacgcagga atggctccgg acttaagctg acgccggtca cccgttgtga
     9061 cagatgctca ggcggaaaca tccggcccca ccgatggatt ttttgagcaa gccacctgcg
     9121 caagaccatc aactgtccgg ccgcgatttc atcttcaagg tgcggaatct ccactttggc
     9181 ctgctcgtag aactgcacgg catacaggtt ccctaacgtg taagtcggga aatagccgaa
     9241 cccgcccatg gaccaatgca cgtcttgcag cacgccctca gcgtcgttcg acggagacac
     9301 gcctaagtat tccttcatct ttgaattcca gatggcaggc aggtcgtcag ggttcgtgtt
     9361 gccctcgacc aagtcctgct cgatttcaaa tcgcagcatg atgtgcaggt tgtacgttaa
     9421 ctcatcggct tcaacgcgga ttaacgaggg tttgacacgg ttgatgacgg tatagaactg
     9481 gtcgatgttc acggcgcgca attgatgatg gaacgtcgct tggaggatcg ggtagaaaaa
     9541 acgccagaag gcacgtgaac ggccgacgca attttcccac atgcgcgact gactctcgtg
     9601 aatgcccagc gacaccgagt cgccgagcgg cgtgccgtaa tgcgctggat cgaggccttg
     9661 gtcgtacaac ccgtggccgc cttcatggat gcagctgaaa aggcacgacg ggagatcgtg
     9721 ctcaaagacc cgagtcgtca cgcgcacgtc cgtgggatga aacgacgtcg taaaaggatg
     9781 tgcggaaaga tccaaccggc ccttctcgaa atcgtatccc atggcgatga gcaccaaccg
     9841 gccgaattcc aactgccgcg cctgatcgta ggcgtcatag agaaagctgt cgtcgatctg
     9901 aagccgactt tgctggatct tcgtgagcaa tgggaccaag tgggccttca actgcgcgaa
     9961 caatggggcc aacgccgcca ccgttgatcc aggctcgtag gagtccagga gcgcattgta
    10021 gggggacccc gtgaatccca ggaactgtgc ttcctcacgc ttcagcgaca agaccgttcg
    10081 caggctgggc aggaacaatt tgaaattgtt ttgcgctttt gcttcggccc acacctgttg
    10141 cgccaacgag cactcgcgac tcaactgcat gacaaaatct gacggtaatt ttttggaacg
    10201 gctgtaatct cgccacactt cgcgcagcag ggcgcgggac gattcgtccc acagctcgcc
    10261 atcctgttcg agcgggagac cggtgtgcag gtcgagccag ccgccgagca gccgctccgt
    10321 ctcgagggaa accagttttt ggtgggcaat gccttgaagg gtcgcaatct gttctgcgcg
    10381 ggccgcaccg ccgcctgccg gcatgtacgt ttcctggtcc cacgagagca cggaggctgc
    10441 actgttgacg cgctggatct ccagcaaccg gcgagtcaac ggttcaagtg tcttgagggt
    10501 tttcatggtg accgcctcct cagccctacg tgctatgatc gcattctgat cgcgcctgct
    10561 tcgtgcagtc tacgcaccgg cgtgccgatt ttcaaaccga ggagggacta tgggcgaatt
    10621 ggttcccgca gagatcgaag cgtatgccgt cgagcattcg atggaagagt ctgagctgtg
    10681 ccgtgacctc cgtgcggaaa cccatcggca catggagtct ccgcagatgt tggtcggtcc
    10741 cctggaaggg gccttgctga aaatgctgac tcaattggtc cgcgcgaccc atgtccttga
    10801 aatcggcatg ttcacagggt acagcgccct ctgttttgcc gaagccttgc ctcatcacgg
    10861 cacggtgacc acctgcgaga tcgacgaggc atctgccgcg ctggcccgtc gttattttgc
    10921 tcggtcacca catgggggta agatttccat ccgcatgggc ccagccattg agacgatgcg
    10981 gacacttgag agacagtatg atgtcatctt catcgatgca gacaaacaga actacgtgaa
    11041 ctactatttg cgtgggaagg agttgctcgc gccggagggg gtcatcttga ttgacaatgt
    11101 gctatgggat ggagacgtcc tggtcatgcc gccgccggac gaacgaacag ctgccattca
    11161 ggcagtgaat cgcgtggtgg cttccgatcc cgagatgggt gctgttctcc ttaccgttcg
    11221 ggacggcgtg cttctcgtca aaaggaaaag tgtcgggtga gccatgatca tgcgcgttcc
    11281 ggcaatcaaa catctttcga cggtcggcca tttggctgtt ggacggaagg cgttccgggt
    11341 gatagctcga ttgcctgacc ttcatgacgg atcgtcagcc ccttcccgct tttcagcgtg
    11401 taatgcgtca cctcgtgtcc gacatccact tccagtgttt ggcctttgaa gatcagattg
    11461 aaccggatac agttgaacac ttccggcagg ctcggccaga agcacagttc gccggtgtaa
    11521 tcgcgcattc cggcaaagcc atagacgagc accatccaca cgccccccat ggcggcaata
    11581 tggcatccat ccttgacgtt tcccgcgaca tcgcccaggt ccatcagggt ggccgctttc
    11641 gcatattcca aggccttgtc catctcatca acttcggcag ccatgatgct ttgtatgcac
    11701 gcggatagcg aagagtcgcc ggtcgttagc ggatcataaa acgcgaagtt tcggcgtttc
    11761 aggtcagggg caaactcatg gcctaataag aacatggcca ggacaagatc tgcctgcttg
    11821 atgacctgat ggcggtagat cgtcaggggg tggaagtaaa gaagcaacgg gtatttgtca
    11881 gccggtgtcc gatcgaaatc ccatggctct tgttccaaaa agtgatcgtc ttgtgggttg
    11941 atcttgagcc gttcatcgta cggaacgtac atccgatcgg ctgcctgctt ccactccgtc
    12001 acttctgacg gctccaatcg agtcttatgc acgagggccg tgtacagatc cggtctgcgc
    12061 tgcttgacgc gttcgaccgt ctcggccgcg tagagaagat tttctcttgc catcagattg
    12121 gtgtatacat tgttgtccac gacggtattg tattcgtccg gacccgtgac gctgtgaatg
    12181 ctaaactgtt cgcctttacg gggcgaatag tagccgagcg accgccataa ccgggcggtc
    12241 tccacgagca tttcgacccc ttcatcgaag aggaactctt cctcgtccgt tgcctgaatg
    12301 tattttttta acgcatacat gatgtcggca ttgatgtggt attgtgcggt acctgctgca
    12361 taataggcgg acgcctcttc gccgttgatc gttctccagg gatacatcgc cccccgctgg
    12421 ttcagttgtc gcgcccgctc gcgcgccttg tccaacatgc cgtggcgaaa ccgcaagaga
    12481 tttttcgcga ttcgcggtgc cgtataggtt aaaaaaggca acacgtaaat ttcgctgtcc
    12541 caaaaataat gtccctcata ggccgctccg gtcagtcctt tcgccggcac gccggtattt
    12601 tccgcacgcg cactggcttg caacacatga aatagattga agcgaatggc ttgctgaacg
    12661 gccgtcgttg acgctttcgc gcgggcggga ttggtgatga ccttgacgtc gctgcgttgc
    12721 cagaagtcat tcatgtactg ttcttgcccg ctcagcaagg cgggaaatcc tgcggcaacc
    12781 gcccgatcga gcgtacgttc ggctcgcgcg cacatttcct cgggcggcgc cgtctgactc
    12841 gtgtggtaca ccatgtactt caccagcgta attggacaac ccggctccgc atcaatcgtg
    12901 gccacggctt ggcctgcatc cggcgtatac ttcgtgttcc acgtcacgtg cgatgtggtt
    12961 tccagcctat gatcgatggc gcaggcgaca atcaggccgc tcttgtttgt ggcatgacag
    13021 aggacgatgc gctgctcctt ggcgtaactc atccgatgat tgagcacttt ctcgacaaac
    13081 actttggcct gtctcgggtc gtaattagcc cggcggggag cgggttcatt ggcaataatt
    13141 tctgacgaaa gaaccaccgg agcggcagca ttcaagagag tcacctcgta gaagatcgcc
    13201 gcgacatgcc ggtgctcgaa cgacaccagc ctccgtgatg tgatcgacac ctgcttgcca
    13261 gccggtgttt cccagaggat gtcccggtcc agcgtccctg atttcatatt gagtcgtcga
    13321 tcaaaacttc gcgcattgcc gtgcggaagc cagaacggtt cgtcatccac atacagcttg
    13381 atgattttgc tgtccgtcac attgacgatt gtttgtcccg ctgttgccaa cccgtaggca
    13441 tcttccggat acacgatcgg ccagctttcg tagaaaccat tgatgaaggt accgttctgg
    13501 ccaatcggcc cgccttcttc gaagcatccc cgcatgccca gatagccgtt tcccagggca
    13561 aacatggtct cgccctgctc aagcaaggcg ggcatgtagc gtttctcaat gaggttccac
    13621 tcatccggtg gatagatatg aggaggcgga tcgaatcggg ggcgatgaat cataggaaat
    13681 ccgcgtggag agccgaccta cgtatggacc gacggtgtgg aagaatctgc ggtgtccatc
    13741 agaggcgaca acaattcgcc caaatctgcg acgacgattt ccgctccctg ttcccggagc
    13801 gcttgagcct gattgtgccg atcgaccccg atgaccagcc caaaaccgcc tgcatggccg
    13861 gcttggacgc cggccaacgc atcttcaatc acgatcccac gccgcggttc aatggccaac
    13921 cttcgagcag cctcgaggaa agaatccgga gcgggcttcc ctgccagctg cagacgatca
    13981 atttcatggc catcgactcg gacatcaaag agcgagctca agccgacggt ctcgatgatc
    14041 tcggcgcaat gatggctcga ggacacgacg gccattttca atcccatggc tcgtgccaga
    14101 tgtaggaact tgaccgtgtt gggataagcg atgacgccgg tctcacgcag cgcgacttta
    14161 aaatgcttgt cctttctttg gccgagtcca tagagcgttt cacggtcggg cccgtcgctg
    14221 ggtgtccctg gcggaagact caccttgcgg gacgccaaga aatcccgcac gccctcatgg
    14281 cgaggtttgc cgtccacata cacccggtag tcctcttcca gatcgaacgg cttgaatgga
    14341 gctcctgttc gtgcgaccac ctcctgtaaa tagtcgtcaa acagacgttt ccaggcagcc
    14401 gcatgcaagc gggccgtctg ggtgatgacg ccatccagat cgcataagac cgcgccaaac
    14461 tctttgagat tcagccgcat gttagaagcc ggtgatccga tatgagctag tgtacagttc
    14521 catcaaacgg aattggattc cggaaaacac ctggcaaatt cccgagcgca gccacgcatg
    14581 gtccgctcaa gaatatgctt gaccgttggc cggaaggatt gtatccccac ggcatggcca
    14641 tacaacctgc cgggttcggc tatggagcga tcgtcgtcgc ggctgtcttg tggggcggat
    14701 ccatcgtcgc gcaaaagctt gcgctgagcg gcttttcagc cgtggaagcc tccgtgcttc
    14761 gggatatcgg cggactcata atcctaatga ccacctggtg gattcaggat ggatcactgc
    14821 gaggaataac gagacaagat tatcgaacac ttgctctcct cggcctgggg gtgctcggca
    14881 accatctgct aatccttatc ggcctcaagt atgtcagcgg ggcggtggcc ggtgtgatca
    14941 tcggggccct gccggtagtg actgccttgt tgtcagcggt cctgagtcaa gacgtcccac
    15001 tgcggcttgt gtgggcaggc agtgtcctgt cgttctttgg agtcgcattg gtttccgtgg
    15061 caggattcaa tgctgcgggt gaacagcctt tgctgggtgg agggctggtg tttctgggcg
    15121 tggcgagttg ggcgttgtac agcatcggca gtcgaacgat catggaacga gttccggcac
    15181 tgaccgtcaa ttggacgacg ctcctggcgg caacagttct gcaaatcccg ctcctctgga
    15241 cagaccagaa gatggtgagg gccgggttgg catcggtgac gcttttggat tggctcgcct
    15301 tagcttatct ggtggtattt gccaccgccg tcgcgcaaca ggcctggctg ttcggcgtca
    15361 aacgcatcgg cccatcacgg gcctccgtgc ttggcaatct cacgcccgtt tccgcagtcg
    15421 cgctctctgc ggtgattctt aaggaacccg tcggcgtagt ggaaatcatc ggtattgtac
    15481 tgatcctcgc gggcgtgtgg gtcgtgcatc ggcagacatc gaacctgcca tcctcttagc
    15541 cgacggcaaa ctcctctcgc gctgctgcag tggattccag tatgatagca cgtttgactg
    15601 aagtgacggt ctactgaatg ctcgagtgcg tgctcgagat gcatgggctg ggacgggaga
    15661 ggaagccatc gtttgccggt gagtgtgcgc atgctggaac ggcggatcga agaagtcaca
    15721 aaggcgaacc agaggtttta cgaagccttt gagagcctgg atatcgcgaa gatggatgag
    15781 atatgggcgc acctggacta cgtcacgtgc gtgcatccgg gttggaccct ccgttcggac
    15841 tggcctgctg tgc
//
LOCUS       DASSCQ010000128         9186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1135559, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000128 DASSCQ010000000
VERSION     DASSCQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135559"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1204
                     /locus_tag="VFH05_08965"
     CDS             338..1204
                     /locus_tag="VFH05_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HET6678388.1"
                     /translation="MAVSARNGHPGCEHVDAHVVERLAKGFRHDSAEDTKASHTSIIS
                     RGPDVERDVALGSSDLREGLHVVPYRQFGVFVYRSRRVNLAIRCGRGDVNRIGNHAHN
                     DNLSFEFALDGTPVITDPGTYVYTPSITMRNRFRSTGMHNTLVLDGKEQNDWKEEREG
                     LFQLQDRSNAKVIDCQATRFVGEHSGFGVPHRRELWISETGIHGRDQCEIEGQKQVMF
                     HVAPSLRTSLSSDGTSVELSKESCIVTLHSLSGAWSLVDSFHSNGYGSIQPATVLSLR
                     TMARTVEWTIQA"
     gene            1201..1983
                     /locus_tag="VFH05_08970"
     CDS             1201..1983
                     /locus_tag="VFH05_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6678389.1"
                     /translation="MNSGMKSEFINEAVKAFWEQEACGTQESIIGTLPELSPAWFRQI
                     EAYRYAMEPYIHSIAQFTRHSGSTVLEVGVGAGTDHVQWARAGARCYGVDLTQKAIDT
                     TEAHLSLYGLQSSLHRLDAETLPFPNNFFDVVYSWGVIHHAASPERIIAEIRRVLKPG
                     GTFLGMMYGRHSLVACKLWIKYALFQGSPWKSLTHVIAHHMESPGTKAYTCRELKVLF
                     SEFSTVHATPIMTVYDRKRLPSWFCKWIPDDFGWFIGLQATR"
     gene            2038..3201
                     /locus_tag="VFH05_08975"
     CDS             2038..3201
                     /locus_tag="VFH05_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6678390.1"
                     /translation="MSRKKLIVASANHWHSPFQVGSHHLARGFVNAGWDVAFVSDPLS
                     PWHVLGGNYTRLQERYRLYADGGHWDLDGHLWAYVPAAILTPHNKPILKSRWVARYWS
                     RAAWPRIGSVLRQAGFGSVDLVYCDSVVPFSWLQKVPHRKSLYRIADQYAAFGKFSAA
                     MVELEKDVAQWVDAVVYASRSLEEHAKSLRPRNLAHLPNGVNFDHFSLERTEIPKEYG
                     SIPKPIATYVGAMDVWFDWDLVNSAVERLPHVSFVLIGPDERARRVLKPARNLFLLGS
                     RPYQELPSYLQHADVGLIPFDVAHYENLVRSIHPLKLYEYLASGLPVVAVEWDELRWL
                     KSPARLTHNHDEFVEGIRQSLATPPDKTSLKRYAADKDWSLIVRQLIEIVDCS"
     gene            3234..4451
                     /locus_tag="VFH05_08980"
     CDS             3234..4451
                     /locus_tag="VFH05_08980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6678391.1"
                     /translation="MLNVVVVSDFAHVEGGNSAVALSSAIGLARSGHAVTLLSAVRPV
                     EPGLFESGIRVICTDQSAIGNDPQRIRALIQGIWNRRAAQSMANILRGFDPGNTVVHV
                     HGWTKALSSSVIKVAIDHRFKVVCTLHDYFSACPNGSFFNYQKNAVCELRPLSGACVS
                     TQCDRQHYGHKIWRLVRQYTQNSWGGMPKDVHHFIMVSEVSRIVLRPYFSPDSQMYFV
                     QNPVRAAYAEPVDISRNSAYLMVGRIAQEKGPHIFAEAARMAGCEAVLVGDGDKCAEV
                     LRLCPSATMLGWLAHEAVMAELRKARALVFPSLLYETDGLSVVEAAALGIPAIVSDVS
                     AARASIVDGVTGLLFKAGDPKDLAEKMSALRDDETGARMGRAAHAHYWKNPRTLERHV
                     QELEHTYEQVLAC"
     gene            4445..5362
                     /locus_tag="VFH05_08985"
     CDS             4445..5362
                     /locus_tag="VFH05_08985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6678392.1"
                     /translation="MLISDQMVSILVPTYNAGPSFRALLDALSRQQLRLFEIIVVDSS
                     SSDDTLSVAKSRGVQTHLIAKQAFDHGGTRNFMARLAKGDILVYLTQDALPVDDTSVP
                     ALVSGLLTDHRTAAAFGRQIPHANATAFAAHHRTFNYPAVSHTRAIQDCEQYGMKTFF
                     CSNSFAAYKRKALESIGWFKEGLLMGEDMYVCAKMLLHGYHIAYAAEAVVYHSHNYSV
                     PQEFKRYFDLGAFFSREPWILERYGNANSEGVRFVRSELSFLRNRGLTRFIPVSLLRN
                     AAKWCGYQLGLLYPYLPSWLIRRVSMHSH"
     gene            5406..6800
                     /locus_tag="VFH05_08990"
     CDS             5406..6800
                     /locus_tag="VFH05_08990"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET6678393.1"
                     /translation="MLKRHSQFLQSVLFLFDLTVICACWIASYHLRFLEGLAPVDKGV
                     PPLDIYLSLLLPIVFVWGLSFRAFNLYRPRRMGSHVAEFFDIAKANTLSVLVLVALTF
                     FVRQFEYSRLVFFYFWILNLVTLGFSRMAFREGLRFIRKQGFNQRHCLIVGAGKLGQR
                     VARLIAIHSEFGIKVQGYLTRHPNKLGRTYEGITVTGLYSEIEKYAPALDIVFLCLPP
                     DEEQGAEKMLQFLATTTIEVKVIPSIYEFITLRAEAEMFEGLPVITLQGSPLYGWNVV
                     LKSITDFVGAAVALTLTAPLMVVIALAVKLTSPGPVFYRQERMGIDGKPFMMLKFRTM
                     HIHQPGDTVLLTPKDDPRITTVGRVLRKTSLDELPQFLNVMKGEMSIVGPRPERSWVV
                     EEVRKQIPLYMLKHKMKAGITGWAQVNGWRGDTSLDKRIEYDLYYIEHWSVWFDVKIA
                     LLTIWRGFVHKHAY"
     gene            6881..7762
                     /gene="rfbA"
                     /locus_tag="VFH05_08995"
     CDS             6881..7762
                     /gene="rfbA"
                     /locus_tag="VFH05_08995"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET6678394.1"
                     /translation="MTRKGIILAGGSGTRLYPVTQAVSKQLLPVFDKPMIYYPLSTLM
                     LAGIRDILIISTPQDTPRFEQLLGKGDRWGLNLQYAVQQSPDGLAQAFIIGASFIGKQ
                     PSALVLGDNIFYGHDFHLLLRNAMARTVGASVFAYHVNDPERYGVAEFDMNGKVISLE
                     EKPKQPKSNYAVTGLYFYDQQVSELAKDLKPSARGELEITDLNRAYMEQGQLNVQIMG
                     RGYAWLDTGTHETLLSASQFIATLENRQGLKVACPEEIAYRQKWISAEQLEQLARPMS
                     KNGYGLYLLGVLKETVF"
     gene            8034..8783
                     /locus_tag="VFH05_09000"
     CDS             8034..8783
                     /locus_tag="VFH05_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6678395.1"
                     /translation="MVKREVLGRYRGSTLGLAWSFFYPVVMLGVYTFVFSVVFKARWG
                     IGGEESKANFAIILFVGMIVHGLFAECVNRAPGLILSNVNYVKKVIFPLEILPWVAMG
                     SALFHAVISLAVLLIAQFVLNFSLPWTAIFFPLVILPLVIATMGFAWVLSATGVYVRD
                     ISQMTGIITTVLLFVSPVFYPVSALPIEYRGWLLLNPLTFVIEEARNVLIWGRVIDWP
                     RWGISVLAGFTIAWIGFWWFQKVRKGFADVL"
     gene            8773..>9186
                     /locus_tag="VFH05_09005"
     CDS             8773..>9186
                     /locus_tag="VFH05_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6678396.1"
                     /translation="MSFEAGTEHRRVQDQADDAAICVHKVSKCYHLYDRPHDRLKQFI
                     VPHVRRLMGREPKNYAREFWALSDVSFNVKKGETVGVIGRNGSGKSTLLQIICGTLTP
                     TSGTVNTNGRTAALLELGAGFNPEFTGRENIYMNGA"
BASE COUNT         2006 a         2376 c         2572 g         2232 t
ORIGIN      
        1 cgttttggcc ttaggcaaag agaaatgtac agtgctgagc acatatgatc atacccggca
       61 cgccgcacaa cctccgctca gacctgcacc gatccgcgta tatcctcgat ctgggcagca
      121 tgccgcgatc ccattcccac cgtcgtattt cagtcgaatc ggtttaatgg ctcaatttac
      181 acaagacatg acaaggcctg atggtcgtat tacgcaaatc ggcgactgcg acaatggtcg
      241 ctttctgaaa cttcaccctg cttatgacga gaatgagtca acgttggaag aggaccacct
      301 caaccatagt catctcgtgt ctgcaatagg aggcctgatg gctgtgtcag cccgcaacgg
      361 ccatcccggt tgtgaacacg ttgacgcgca tgtggtcgaa cgccttgcga aaggatttcg
      421 gcacgactct gccgaagata cgaaggcgtc ccacaccagc atcatctcga gaggaccgga
      481 cgttgagcgc gatgtcgcat tgggatcaag cgatcttcgt gaaggattgc acgtcgtgcc
      541 gtatcggcag ttcggcgtat ttgtatacag atcgcgccgg gtgaaccttg ccatccgctg
      601 tggtcgcgga gacgtcaata gaatcggcaa ccacgctcac aatgataatc tatctttcga
      661 attcgcactc gacggcacgc cggtcatcac cgatccggga acctacgtct acactccgtc
      721 catcacgatg aggaatcgtt tcaggtctac tggaatgcac aatacgttgg tgctggatgg
      781 caaagaacaa aacgactgga aggaagaacg agaagggctg ttccagcttc aggataggtc
      841 gaatgcgaag gtcatcgact gtcaggcgac tcgctttgtg ggagaacatt ccggatttgg
      901 tgtgcctcac cgccgtgagt tatggatttc ggaaaccggg atccacggac gcgaccaatg
      961 tgaaatagag ggacagaagc aggtaatgtt tcacgttgcg ccatccctac gaacatctct
     1021 ttccagcgac ggcacgtcgg tcgagctatc gaaggagtcc tgtattgtta ctcttcacag
     1081 cctcagcgga gcatggtcgc tcgtggattc ttttcattcc aatggatatg gatcgatcca
     1141 gccggccact gtgctttctc tcagaaccat ggcgcgcacg gttgaatgga ccatccaggc
     1201 atgaacagcg gcatgaaaag cgaattcatc aacgaagcag tcaaagcttt ttgggagcag
     1261 gaagcttgcg gaactcaaga atcgattatt ggaacccttc cggaattgtc tccggcctgg
     1321 tttaggcaga tcgaagcata ccgatacgcc atggagccct acatacactc catcgcacaa
     1381 ttcacccgtc attccggaag cacggtgttg gaggtaggtg tcggcgcagg caccgaccat
     1441 gtgcagtggg cccgtgcggg ggcgaggtgc tatggagtcg acttgacgca gaaagcgatc
     1501 gacacgacgg aggctcatct ttctctctat ggattgcagt cgtccctgca tcgacttgat
     1561 gcggagactc tgccttttcc caataatttt tttgacgtcg tatactcatg gggagtcatt
     1621 catcacgctg cgagtcccga gcgcatcatt gcggaaataa ggcgcgtcct caagccagga
     1681 ggcacatttc ttggcatgat gtatggacga cattcgttgg ttgcctgtaa attgtggata
     1741 aaatatgcct tgtttcaggg aagcccatgg aaaagcttga cgcatgtcat cgcccatcac
     1801 atggaaagcc cagggaccaa ggcctacaca tgccgggagc tcaaggtcct gttctcggag
     1861 ttttcaaccg ttcacgcgac accgattatg acggtgtacg atcgcaaacg attaccctct
     1921 tggttttgca agtggatccc tgacgacttc ggatggttca ttgggctgca agcgaccagg
     1981 tgatgtggat gttgcagagt ctatggtctg ccgtcacgtc gagatcctgc cttcgtggtg
     2041 agtagaaaaa aactgatcgt ggcgtccgcc aatcattggc actcgccctt ccaggtgggg
     2101 agtcatcatc tcgcccgcgg gtttgtcaat gccgggtggg atgtcgcctt tgtgtccgat
     2161 ccgctttctc cctggcatgt gctgggcggc aactacaccc ggttgcagga acggtatcga
     2221 ttatatgccg acggcggcca ttgggatctc gatggccacc tatgggccta tgttccggcc
     2281 gccatactca cgcctcataa taagccgatt ctcaaaagtc gttgggtcgc tcgctattgg
     2341 tcacgtgcgg cctggcctcg aatcggatct gtgctccgtc aggcgggttt cgggagcgtg
     2401 gatctggtgt attgtgacag cgtggttccg ttcagttggc tgcagaaggt tccacacaga
     2461 aagtcgcttt atcggatcgc agatcagtac gcggcctttg gcaaattctc ggcggcaatg
     2521 gtggaattag aaaaagatgt ggctcagtgg gttgatgcag tggtctacgc ctcccgcagt
     2581 ttggaagaac atgcaaagtc attgaggcct cgcaatctgg ctcatctgcc aaacggcgtg
     2641 aatttcgatc atttttccct cgagcgaacc gagatcccaa aagaatacgg aagcattccg
     2701 aagcccattg cgacgtacgt gggagcgatg gatgtctggt tcgactggga cctcgtcaat
     2761 tctgccgtcg agcggctgcc tcatgtctcg tttgtcctga tcgggccgga cgagcgtgca
     2821 aggagggttc tcaagccggc gaggaatctc tttctcctcg gctcgagacc ttaccaagaa
     2881 ttgcccagtt acctgcagca tgcggatgtg gggttgatcc cttttgacgt ggcccattac
     2941 gaaaacctgg tgcgcagtat ccatccgctg aagctctatg aatatctggc cagcggactg
     3001 cctgtcgtcg cggtcgagtg ggacgagttg cggtggctga agagtcctgc gagattgacg
     3061 cataatcatg atgagttcgt cgagggcatt cgtcagtcgc tcgccacacc gcccgacaaa
     3121 acgtctctca aacggtacgc ggccgacaaa gactggagtc tcatcgtccg gcagctgatc
     3181 gagatagtgg attgttcgta gcgagtggcc ttctgttcgc agcccgtcct cccatgctca
     3241 atgtcgtcgt ggtgagtgat tttgcccatg tcgaaggcgg gaactccgcc gtcgcgctgt
     3301 cgagcgccat cggtctcgca cgcagcgggc atgctgtgac ccttctgtcg gcggttcgtc
     3361 ctgttgagcc cggcctgttc gagagtggga tccgggtgat ctgtaccgat caaagcgcga
     3421 ttggcaacga tccgcaacga atccgtgcgc tgatccaggg aatttggaac aggagagccg
     3481 cgcagtctat ggccaacatc ctccgtgggt ttgatccggg gaataccgtc gtgcacgttc
     3541 atggctggac gaaggccctg tcgtccagcg tcatcaaagt tgcaatcgac catcgcttta
     3601 aagtcgtgtg tactctccat gattactttt ctgcatgtcc caacggcagc ttttttaact
     3661 atcagaagaa cgccgtctgc gaacttcgtc cgctctcagg cgcttgcgtc tcgacgcaat
     3721 gcgacagaca gcactacggc cacaaaatct ggcgccttgt gcgacagtac acccaaaata
     3781 gttggggggg aatgccgaag gacgtccacc atttcatcat ggtatcggag gtgagcagga
     3841 tagtcttgag gccctacttt tcccctgatt cacaaatgta ctttgtgcaa aacccggtgc
     3901 gagctgccta tgcagagcct gtcgacatca gtcggaattc agcgtatttg atggtgggaa
     3961 ggattgcgca agaaaaaggg cctcacatat ttgccgaggc ggcgcgcatg gcaggctgtg
     4021 aggctgtgct tgttggcgat ggagataagt gcgctgaggt ccttcgcctg tgcccctctg
     4081 ccacaatgtt gggctggctc gcgcatgaag cagtcatggc ggaattacgc aaggcccggg
     4141 cgctggtgtt cccatcgctg ttatacgaaa ctgacggtct ttcagtcgtt gaggcggcag
     4201 ccttagggat tcccgcgata gtctcggatg tctcggcggc ccgggcctca attgtcgatg
     4261 gggttaccgg cttactgttc aaagccggcg atccaaaaga ccttgctgag aagatgtcgg
     4321 cattacgaga cgatgaaaca ggagctcgca tgggtcgagc ggctcatgcg cactattgga
     4381 agaatcctcg gactctggaa cgtcatgtcc aggagctcga acatacctat gagcaggtgt
     4441 tggcgtgctg atatcggacc aaatggtcag tatcctggtc cccacttaca atgcgggccc
     4501 ttcgttcagg gcattgcttg atgcgctcag ccgtcaacag cttcggctgt tcgagattat
     4561 tgtcgtcgac tcctcttcga gcgacgatac gttgtcggtc gcaaagagcc gcggagtgca
     4621 gacccatctc attgccaaac aggcgttcga ccacggcggg acacggaatt tcatggctcg
     4681 actggccaaa ggcgatatcc ttgtctatct gactcaggat gcgctcccgg tggacgacac
     4741 gtcggtgccg gcgctcgtgt cggggcttct gaccgaccac cgcaccgcag cggcgttcgg
     4801 tcggcagatt ccccatgcga atgcgacggc cttcgcggcg catcacagga cctttaatta
     4861 tccggcggta tcgcataccc gagcgattca ggattgtgaa caatatggta tgaaaacctt
     4921 cttctgttcc aactcctttg ccgcctataa gcgaaaagcc ctagaatcca tcggctggtt
     4981 caaagaaggc ttactgatgg gcgaggacat gtatgtctgt gccaagatgc tgctacatgg
     5041 gtaccacatc gcgtacgccg ccgaggcggt cgtctatcac tctcacaact attcagtgcc
     5101 acaggaattt aagaggtact tcgacctggg cgcctttttc agcagggaac cgtggattct
     5161 cgaaagatat gggaatgcca actcagaggg tgttcgattc gtccgatctg aactatcgtt
     5221 cctccgcaat cgcggcctta ctcgcttcat tccagtaagc ctactccgca acgcagccaa
     5281 atggtgcggg tatcagctcg gactgttata tccgtatctt ccgagttggc tcattcgacg
     5341 ggtcagcatg cattcacact agcctgtgct gttgcggcga atgttaggtt aagcgagatg
     5401 ctctgatgct caaacggcac tcacagtttc tgcaaagtgt gctctttctg tttgatctca
     5461 ctgtgatttg cgcttgttgg atcgcctcgt accacctgcg gtttctggag ggcttggcgc
     5521 cggtcgacaa gggcgtccct ccactcgata tttatttgtc gctgcttctg ccgatcgtct
     5581 ttgtgtgggg gctttccttc cgagccttca atctctatcg tcctcgccgc atgggatctc
     5641 atgtggcaga attctttgat attgccaaag cgaatacgtt gtcggttctt gtgctggtgg
     5701 cgctcacctt cttcgtccgt cagttcgagt attcacgact cgtcttcttc tatttttgga
     5761 ttctcaatct ggtgaccttg ggcttctctc gcatggcgtt tcgtgaagga ctgcgcttta
     5821 tccgcaaaca aggcttcaac cagcggcatt gtctgatcgt cggcgcaggg aagctggggc
     5881 agcgggtagc cagattgatc gccatccata gcgaattcgg aataaaagtg cagggatacc
     5941 tcacgcgaca tcccaataag ctcgggagaa cctatgaagg gatcactgtc actggtctct
     6001 attcagaaat tgagaaatat gcaccggcat tagacattgt gtttctctgt ttgcctcccg
     6061 atgaggagca aggcgcggag aaaatgttgc agtttctggc cacgacgacg attgaagtaa
     6121 aggtcattcc atccatttac gaattcatta ctttgcgggc cgaggccgag atgtttgagg
     6181 ggttgccggt gattaccttg cagggatcgc ctctctacgg ctggaacgtg gtcttgaaga
     6241 gtataaccga ttttgtgggc gcggcggtgg cactcacgtt gaccgccccg ctcatggtcg
     6301 tcattgcgct ggctgtgaag ctcacatctc cggggcccgt gttttatcgt caggaacgga
     6361 tgggcatcga cggaaagccg ttcatgatgc tgaaatttcg aaccatgcac attcatcaac
     6421 caggtgatac cgtgttgctg acgccgaagg acgatccgcg gattacgacc gttggccgag
     6481 tacttcggaa gacgagcttg gatgagttgc ctcagttcct gaatgtcatg aagggagaga
     6541 tgagcattgt cgggccgcgt cccgaacgtt catgggtcgt ggaggaagtc aggaaacaga
     6601 ttcctctgta tatgctcaag cataagatga aagccggcat aacagggtgg gcacaggtga
     6661 atgggtggag gggagatacg tcactcgaca aacggattga atatgacttg tactatatcg
     6721 agcactggtc ggtgtggttc gatgtgaaga ttgcgttgct cacgatatgg cgcggattcg
     6781 tgcataaaca cgcctattga acagagtcct gccgtaaggc atgaggacct catggtgagg
     6841 aatcacaggt gtcctttctg ggagaccgaa tagggaaagc atgacacgca aaggcattat
     6901 tctagcgggc ggctcgggta ccagacttta tcctgtgacg caagcggtct cgaaacagtt
     6961 gttgccggtc ttcgacaagc cgatgattta ctacccgttg agcactctga tgctggctgg
     7021 catccgagac attctcatca tctcaacacc acaggatact cctcgtttcg aacaactcct
     7081 tggcaaaggg gatagatggg gcttgaactt gcaatatgct gttcaacaat cccccgacgg
     7141 cctggcgcag gcctttatca ttggcgcctc ttttattggt aagcagccgt cagctctcgt
     7201 gcttggcgac aatatattct atggtcacga ttttcatctc ctgcttcgca acgcaatggc
     7261 tcgaacggtg ggggccagtg tcttcgcgta tcacgtcaac gacccggagc gatatggagt
     7321 ggcagagttc gacatgaacg gaaaggtgat atcgctcgaa gaaaagccga agcagccgaa
     7381 gtcgaattac gccgtcacgg gtttgtattt ttatgatcag caggtgagtg agcttgccaa
     7441 agatctcaag ccctcagccc gtggcgaatt ggaaatcacg gacctgaatc gcgcatacat
     7501 ggaacaagga caacttaacg ttcagatcat gggtcggggg tatgcgtggc ttgataccgg
     7561 tacccacgaa accctgctga gtgccagcca gttcatcgcg acgcttgaaa atcgccaagg
     7621 gctgaaggta gcctgcccgg aagagattgc ctataggcag aagtggataa gcgccgaaca
     7681 gttggagcaa ctcgcccggc cgatgtccaa aaacggctac ggcttatact tattgggtgt
     7741 gctcaaggaa acagtttttt aaccagttca aacctcacga gcgagtcttc gtcgatagtg
     7801 ttgcaatggc atgtcgccct atcgtcttgc cggagttggg aagtcgattt tcgtcataac
     7861 cgctcctgca tgaacgacac gacacaacac ctagaaacac tcgtagcagg gaatgtgctc
     7921 gctctacaga gaagcgtttg caccaccctc acgtggttct gatgcagcgc ttctctacca
     7981 ctcccatgga aatgattgcg agtgtctggc gaaatcgatg gcttactata caaatggtca
     8041 aacgtgaggt gctgggtcgc tatcgaggat cgaccctggg actcgcctgg tcgtttttct
     8101 atcccgtggt catgctgggg gtgtatacct tcgtattttc cgtcgtgttc aaggccagat
     8161 gggggatagg aggcgaggaa agcaaggcta attttgccat cattcttttt gtcgggatga
     8221 tcgtccacgg tctattcgcg gaatgtgtca atcgcgcccc tggcctcatc ctctcgaacg
     8281 tcaattatgt gaagaaagtc atatttccgc tggagatttt gccgtgggtt gccatggggt
     8341 cagcgttgtt tcatgccgtc attagcctgg cggtgttgtt gatcgcgcag ttcgttctga
     8401 acttctcttt gccctggacc gcaatttttt tcccccttgt tatcctgcca ctcgtcatcg
     8461 cgaccatggg ctttgcgtgg gtgctctccg ccacgggggt atacgttcgc gacattagtc
     8521 agatgaccgg gatcataacc accgtcttgc tgtttgtatc gccagtgttc tatcccgttt
     8581 ctgctttgcc cattgaatac cggggatggc tgctgctcaa tccgctgacg ttcgtcatcg
     8641 aggaagcacg aaacgtcctg atatggggaa gagtgataga ttggccgaga tgggggatct
     8701 cggttctagc cggctttacc atcgcctgga taggattctg gtggtttcag aaggtccgaa
     8761 aggggtttgc tgatgtcctt tgaggcgggc accgaacatc ggcgagttca agaccaagca
     8821 gatgatgctg cgatttgcgt tcacaaagtg agcaagtgct accacctcta cgatcgcccc
     8881 catgatcggc tcaaacaatt cattgttccg cacgtgcgac ggctcatggg cagggagccg
     8941 aagaactatg ctcgagaatt ctgggcatta agcgatgtat ccttcaacgt gaaaaaaggc
     9001 gaaacggtcg gcgttatcgg acgaaatggc tccggtaaat ccactctgct gcaaatcatt
     9061 tgcggcaccc tgacgccgac gagtggaaca gtcaacacca atggtcgtac ggcggcactg
     9121 ttggaacttg gggcagggtt caatcccgaa ttcacgggtc gcgagaacat ttacatgaac
     9181 ggtgcc
//
LOCUS       DASSCQ010000129        10840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_639414, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000129 DASSCQ010000000
VERSION     DASSCQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639414"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..910
                     /locus_tag="VFH05_09010"
     CDS             347..910
                     /locus_tag="VFH05_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678397.1"
                     /translation="MRMLGRELLAVLTMGCLAITAVSAAETPSEAQLERLMQMASQTP
                     DRPDHDYVSNSLQALLQQSQDLKLSREQTNKIKSITDQYERTRRDRETAYKRSEMEAL
                     KLIHDNRSSLFSIETAVQKADQEHSKLRMAGIKALREARDVLGPEQYGNWRQSHAASQ
                     LAQGSPHQERGEQYDSESSGSGRVAPH"
     gene            981..1532
                     /locus_tag="VFH05_09015"
     CDS             981..1532
                     /locus_tag="VFH05_09015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6678398.1"
                     /translation="MRRFNLFVVVSALVGLLIPPPMLVVSAENEPIIQDGSVVLMEFT
                     ITVPESRLVIPKSVSQFTPGHHEMLPNLEKALTGMKKGDEKRVDLSSDDAFGPYDETK
                     KGVISAESLPPDTKPGTILTTEEGVPFVVTELSGPVASVDFNHPLAGKHLIIDVKILN
                     VESPSGQEGMRMGSQEEYKDISI"
     gene            1550..2182
                     /locus_tag="VFH05_09020"
     CDS             1550..2182
                     /locus_tag="VFH05_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET6678399.1"
                     /translation="MLPDQPYKTKSFNLHGLQGISDKTIDTHLSLYEGYVKATNALQG
                     RLAQFLQDGNVDQEEFPAYSELTRRLGFEFNGMVLHEYYFENLTPQASIKPDSNSAFL
                     QQVEKSFGTYERWKTDFVSVGKMRGVGWAICFRNPSSGRLSNHWITLHEHGNVAGFQP
                     VLVMDVWEHAFLLDYSPSQRGEYINAFFANVNWRVVDGRIGSDAPNLRAA"
     gene            complement(2247..3575)
                     /locus_tag="VFH05_09025"
     CDS             complement(2247..3575)
                     /locus_tag="VFH05_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6678400.1"
                     /translation="MTTDAVERQQDVKAPTSRMPGWQLLKERDFSLLFWGQMTSQIGD
                     SLNRVALLWFVYQLTGSAMKMVMIGLLQTIPPLVLSPLIGVYLDRANKKTVMVWVDIL
                     RSALVLLIPLLYAFDQLTLERLYAAVFLLAVVSTIFGPALSASVPQLVRRDQLTAANA
                     LLQTTTNTGLLIGPLISGVGIALIGSQNVLYVDAVTFFVSALCLIGIRMRPLDKTQLE
                     KGRGTWTQELLAGIRFVYLEHTVLVLMVTAALYSLAASAFAFMLPVFAEQNLSAGALE
                     VGALWSALGAGMLATSTWLASLTQGDLHGRFTLIFRSMAVGGLAMCGLSMLTAPLIAG
                     ALILIIGGSTAIFMPIVWGVLQEVTPAPLLGRVFTTFSTGSMASAMAGMAGFGWAADT
                     MGPHVSLLGIGLVLLCTACLAFLSCRRFPSRPRPAPILSPLDASEQKLAA"
     gene            complement(3854..5230)
                     /locus_tag="VFH05_09030"
     CDS             complement(3854..5230)
                     /locus_tag="VFH05_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6678401.1"
                     /translation="MHPTRLDLDLSLGLFMEDRLTRVIKDLSTHSRQHHRQHHIKRRW
                     LLSADFSYVWWGQVVSQVGDGVSKLALLWFVYSVTGSPLKTSIIGILQTAPAIVLAPI
                     IGVAVDRLPKKALLIVSDLVRAVVIGLIPCWMSVDSFNVSSLYVLVTLHAVATAVFGP
                     ALTASVPSLVVPSQYTSANALLQITTSLGIILGPALSGLGIAALSSQEVLCLNALTYV
                     ISAACFIPIRFAPPVLSLVPHSPIMSAWRDVLEGLRYVLKGRRIIIFLTITASLYTFA
                     TSAFTTLFPVFARKLLDLGPVEVGYLWSMLGIGLLTVSLALTVISAWSVKRRIKVIAV
                     ASAVSGLALLALVMVTHPTEAAALLIVLGGGLGVLTPVAWGVLQEVAPAHMLGRVLAF
                     YTLGAMAAAMGGITLFGWVTGRFGVPAAVVAIGLVMGCTGLSAWRFLDNMSERPPKRE
                     PPLVRDQA"
     gene            complement(5275..6132)
                     /locus_tag="VFH05_09035"
     CDS             complement(5275..6132)
                     /locus_tag="VFH05_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6678402.1"
                     /translation="MFIWPLSGLLVLALISATGYAAEDTGGERAKDTPKTAEPKKKSE
                     GELKSDGNGKPGSEKAGAEEKKADDRKSDEKKADDKKVDDKKVEEKKAEEKKAEEKKA
                     EDKKAEDKKADEKKPDEKKAEEPSPPKPEEHKKPVTSLNLAIKLALMADPQLFPLEIE
                     VEMDKSKAVLTGAVPTEEDKARVAEIAAKVDGVEGVVNKVNVTPALRDKLAKKQDETI
                     AHVVRDRLSRSETLKAVGFDVKSENGIVYLSGKTRFQVIALEAAEAARQVPGVRAVDT
                     AGVQLVAKD"
     gene            6378..7616
                     /locus_tag="VFH05_09040"
     CDS             6378..7616
                     /locus_tag="VFH05_09040"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET6678403.1"
                     /translation="MKPKRILGIIMAGGKGERLLPLTEVRSKPAVPFGGKYRIIDFVL
                     SNFVNSGITSLYVLVQYRSQSLIEHLRHSWQIGGRLTQSFVSVVPPQMRAREGWYEGT
                     ADAVYQNLNLIDDWAPDVIAVFGADHVYRMDIQQMVQEHLEHDADATVAALPVPIEAA
                     GGFGILEADEAGRMVGFEEKPTAPKPMPARQGYALSSMGNYLFKTDTLVPLLKRDASR
                     PGPHDFGRNIVPELLEDHRVYVYNLHNNEIPGLRSYEEQGYWRDVGTLEAYWQANMDV
                     LGEHPLFDLHNTDWPIFSSGYDGPMAALVRTHADDSMIGPGSHCVDADIRKSIIGRGV
                     RIEQGAYLEECVILDDVHIGPHARLRRVIADRRSTVPAYSRIGYDAGHDRKRFHVSHS
                     GLVVLRHPSSTDPHMLRRAS"
     gene            7613..10510
                     /gene="treY"
                     /locus_tag="VFH05_09045"
     CDS             7613..10510
                     /gene="treY"
                     /locus_tag="VFH05_09045"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET6678404.1"
                     /translation="MTAATLSSIPLATYRLQLHHGFTFADAQRAVPYLHALGITDCYL
                     SPISKATPGSDHGYDVIDPVLLNPELGSEEDFQEFVRTVQEHGLGIVLDVVPNHMGIG
                     KTLNRWWRDVLENGPSSRYATAFDIDWHPIKRELENKVLLPILADQYGAVLESQEIEL
                     TYEDGVFFLRYYDHNLPLATKTWTHILSHRLDLLVAEGEQAMPVLELQSILTALKNLP
                     GAAERDPERITEHYREKEIVKKRLAALMDGSPVIRTFVLENVRLFNGERGRSESFDLL
                     DALLNEQAYRLASWKVASEEINYRRFFDINELAAIRMEEEAVFQESHQLVLRLVREGK
                     VRGCRIDHVDGLYDPGQYLRHLREFLQPQPDGAQAPLYLVVEKILGKDEPLPESWPVQ
                     GTTGYDFLNQVNGLFVAGAHEHFFTDVYAKTIDRREDYADLVYGCKRLIMRVSMASEI
                     NVLGHQLNGISERDRRSRDFTLNNLTHAIREIIACFPVYRTYVTESTETVADRDRAYI
                     HMAVARAKRRNPAISGQVFDFIRSILLKQREARTKEDQDEQMRFVMKFQQTTSPVTAK
                     GVEDTAFYRYHRLVSLNEVGGEPEQFGFSVEDFHKRMWERQSRWPHALSASSTHDTKR
                     SEDVRARINVLSEWPQEWKSKVSRWSRLNRPHFRESEGERMPDRNDEYLFYQTLIGAW
                     PLEAMGDEAYQEFCGRIQRYMEKAVHEAKVHTSWINPSPAYDQSMQTFVEAVLERSTS
                     NRFLEDFLPFQERVARYGLYNSLGQLLVKMTAPGIPDFYQGTELWSFHLVDPDNRCAV
                     DYDARAAMAEELRATLKQLGSDRAEFARSLFEQAQDGRVKMYTIMTGLEYRRLHPELF
                     QQGEYLQLECGGSKKQHLCAFARLYQDHALVTVIPRLVATLNPDSKHPPIGLPVWEDS
                     WMVVPPWPTLSRFRNLLTGEIITAESVNGRRVLPLAKVLHHAPIALLERLP"
     gene            10651..>10840
                     /locus_tag="VFH05_09050"
     CDS             10651..>10840
                     /locus_tag="VFH05_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678405.1"
                     /translation="MLEPEYRSGATTIAEESVELDQPSVSPVGWRQWSTRIESQIERY
                     PWPTILLALGCGYLLARRL"
BASE COUNT         2389 a         3090 c         3138 g         2223 t
ORIGIN      
        1 aaatcgagcg gcgtgatgag aacaggtggg aattgcatcc tgcctcctcg gaagactttg
       61 ccgagcgtac tcattcgacc gacatgtcgt cagcaacttc cgaagaggaa ccgtcttcag
      121 cacaaagccg ggaacccggg cccgacaagc gcaaggccgc ctgaggcgaa gcctcgttcc
      181 tcccgctgat ttcagcgaat ccctcaagac aagctgtccc acaccggaca gaatgccagc
      241 gggtgtcggc tatttgtgcc caagttcgga tggcttatgc ctgacaggta ggcggcacat
      301 cccttgctgt ttttacgaga atagctttgt tttgaaggag gacatcatgc gaatgcttgg
      361 gagagaattg cttgcggtct tgacgatggg atgtctcgcg atcaccgcag tcagcgcagc
      421 ggagactccc tcagaagctc aactcgaacg gctcatgcag atggcttccc agacacctga
      481 ccggccggat cacgactatg tcagcaatag cctgcaggcg ttgctgcagc aaagtcagga
      541 cctcaagctc tcgcgggagc agaccaacaa aatcaaatcg attactgatc aatatgagcg
      601 gaccagacgt gaccgcgaaa cggcgtacaa gcgcagtgaa atggaggcat taaaactgat
      661 ccatgacaac cgtagctcac tgttttccat cgaaactgcg gttcagaagg ccgatcagga
      721 gcatagcaaa ctgcgtatgg ccggtatcaa agccttgcgt gaggcgaggg atgtgttagg
      781 accggaacag tatggcaact ggcgccaaag ccatgcggcg agtcagttgg cgcagggaag
      841 tcctcatcag gagcgagggg agcaatatga tagcgagtca agcggttcag ggcgcgtggc
      901 gccccattaa ataccggctg cctgccgact ggtgaacccg ccactggaga cgcagcgcac
      961 aacgttggaa ggagcgcatc atgcgtcgat tcaacctctt cgtggtcgtc tcagccctcg
     1021 tgggtttact gattccccca ccgatgcttg tcgtctcggc ggaaaacgag ccgatcattc
     1081 aagacggctc cgtcgtgctg atggaattta ccattaccgt tccagaatct cggctcgtca
     1141 ttccgaagag cgtgagtcag ttcactcccg gtcatcatga aatgttgccg aatctcgaaa
     1201 aagcgctcac cggcatgaaa aaaggtgacg aaaagcgtgt ggatctgtca tccgacgatg
     1261 catttggccc ctatgatgaa acgaagaaag gcgtgatcag cgccgagagt ctgccacccg
     1321 ataccaaacc gggcacgatt ctcacgacag aagagggagt accgtttgtg gtcacggagt
     1381 tatccggtcc agtggcgtcg gtcgacttta accacccgct ggctggaaag catctgatta
     1441 tcgatgtcaa aatcctgaac gtggagtcgc cctctggtca ggagggtatg cgaatgggat
     1501 ctcaggaaga atataaggac atttccatct gaccagagga ggaacagtca tgctgcctga
     1561 tcaaccctat aagaccaaat ccttcaatct ccatgggctg caaggtatct ccgataaaac
     1621 catcgacaca catttgtcac tgtatgaggg atatgtcaaa gccacgaatg cattgcaggg
     1681 ccgtctcgca cagttcttac aggatggcaa tgtcgatcag gaagagtttc cggcctactc
     1741 cgagctcacg cgccgcctgg gttttgaatt caacggcatg gtcctgcatg agtattactt
     1801 tgaaaacctg acgccccagg catcgatcaa gccggacagc aattcggcct ttctccaaca
     1861 ggttgagaag tcgtttggaa cgtacgagcg gtggaaaaca gatttcgtga gcgttggaaa
     1921 gatgcgcggc gtcgggtggg cgatctgctt tcggaaccca tcatccggcc ggcttagcaa
     1981 ccattggata accttgcatg aacacgggaa cgtggccggt ttccaaccgg tgctcgtgat
     2041 ggacgtgtgg gaacatgcct ttctgctcga ttattctccg tcccagcgcg gtgagtacat
     2101 caatgcattt tttgccaacg tgaactggcg ggtggtggac gggcgcatcg gatcagacgc
     2161 tcccaacctc cgggcggcat agtagccgtc tttgttttgc agtcggaacg ttggcgtttg
     2221 cgaaggtagt gcggctacgg cagcggttat gcagccagtt tctgctctga cgcatccagc
     2281 ggggagagga tcggtgccgg gcgcggccgg ctcgggaacc ggcggcacga cagaaaggcc
     2341 aggcaggcgg tgcacaagag cacgagtccg atacccaaga ggctgacgtg cggccccata
     2401 gtatcagcgg cccagccgaa ccctgccatc cctgccatgg cagaggccat tgaccctgta
     2461 ctgaacgtcg tgaagacccg acccaagagg ggggcgggcg tcacttcctg caacaccccc
     2521 cagacgatgg gcatgaagat agcggtgctc ccgccgatga tcaggatcaa ggctccggcg
     2581 atcaaggggg ccgtcaacat gctgagcccg cacatcgcca gtcctccgac ggccatcgat
     2641 cgaaaaataa gcgtaaagcg tccatgtaaa tcgccttgag tcaaggaagc cagccaggtt
     2701 gatgtggcca gcatcccggc gccaagggcg gaccataacg ctcccacctc cagcgctccg
     2761 gcactcaaat tctgttcggc gaatacgggc agcatgaagg cgaaggcgct ggccgccaag
     2821 ctgtacaggg ctgccgtgac catcaacacc agcaccgtgt gttccaggta gacaaagcgg
     2881 attccggcga gcagttcctg tgtccaggta ccgcgaccct tctccagctg tgtcttgtcc
     2941 aatggacgca tgcgaattcc gataaggcag agggcggaca cgaaaaaagt cacggcgtcg
     3001 acatacagga cgttttgaga gccgataaga gcgattccca ccccgctgat cagcgggccg
     3061 atcaggagac cggtgttggt cgtcgtctgc agcagagcgt tggcagcggt cagctggtcg
     3121 cgccgcacaa gctgtggcac ggaggccgat aaagccgggc caaaaatcgt ggacacgaca
     3181 gccagtagga agacggccgc atacaaacgc tcgagcgtta actggtcaaa tgcatagaga
     3241 aggggaatga ggaggaccaa ggcgcttcgg aggatatcca cccagaccat gacggttttt
     3301 ttgttggctc gatcgagata caccccgatc aagggactga ggacgagtgg cgggatcgtc
     3361 tgcagcaagc cgatcatcac catcttcatg gcggaaccgg tcagttgata gacgaaccac
     3421 agcagcgcga ctcgattgag gctgtcaccg atctgggacg tcatttggcc ccagaacaag
     3481 agactgaaat cccgctcctt caataactgc catccgggca tgcgtgaagt gggggctttc
     3541 acatcttgtt gccgttcaac tgcatcggtc gtcatgctcg gctcctggtc acacacgttc
     3601 gacgatcgag tcttgcacac accttgccac gtctcttgta tcgaatccta tgagggaaaa
     3661 ttgtgccttg tagagcgagt gctccgcaag cgcagaccgg atttcatctt ttcgctcgtc
     3721 ggagacactc tctcaataac gtcgaaaccc ggacaatcaa gacctaggga gagtcctcgt
     3781 tgttggtccc tgcactaatt attcaatacc atacatacgc atggttccat gactcggacg
     3841 ctccctagct ggcctatgcc tgatctcgaa caagcggtgg ctcgcgcttg gggggccgct
     3901 ctgacatgtt gtcaagaaaa cgccaggccg ataagccggt gcagcccatc acgagtccta
     3961 tcgcgacgac cgcggcaggc accccgaatc gaccggtgac ccacccgaag agggtaatcc
     4021 ctcccatcgc ggcggccatg gcgccgagcg tgtagaaggc caggacgcgg ccgagcatgt
     4081 gggccggtgc cacttcctgg aggacacccc aagcgaccgg cgtgaggact cccaaacctc
     4141 cacccaagac gatcaacagg gcagccgcct cagttggatg ggtcaccatg accaacgcca
     4201 gaagggcgag accgctcacg gcgctggcca cggcaatcac cttaatccgg cgcttcacgc
     4261 tccaggcgct gatgacggtg agtgcgagcg aaacggtgag cagtcctatt ccgagcatgg
     4321 accacaagta gccgacttcc accgggccga gatcaagcag tttcctcgcg aacaccggga
     4381 acagcgtggt aaatgcgctc gtggcgaatg tgtacagcga ggcagtgatc gtcagaaaga
     4441 tgatgatccg gcggcctttc agcacatacc ggagcccttc aaggacgtcg cgccaggcag
     4501 acatgatggg agagtgcggc accaggctga gcacgggagg cgcgaaccgg atcggaatga
     4561 agcaggctgc ggaaataacg tacgtgagag cattcaggca cagcacttcc tgtgagctga
     4621 gtgccgcgat ccctaagccg ctcaaggccg gacccaaaat gatgcccaaa ctcgtggtga
     4681 tttgaagcag ggcgttggcg gacgtgtact gagacggcac gaccaaggag ggaacggacg
     4741 cagtcaatgc cgggccaaat actgccgtgg ccactgcatg cagggtcacg aggacgtaca
     4801 gggaagaaac gttaaagctg tccaccgaca tccagcacgg aatgagcccg atcaccaccg
     4861 cccgcaccag atccgagacg atcaagagcg cttttttcgg caggcggtcg acggccaccc
     4921 caatgatcgg tgcgaggacg atagcgggag cggtttgtaa gatgccgatg atgctggttt
     4981 tcagcggtga gcccgtgacc gaatacacga accagagcag cgccagcttg gagacgccgt
     5041 ctccaacctg ggagacgacc tggccccacc agacgtacga gaaatcggca ctaagcaacc
     5101 accgccgctt gatgtgatgc tgacggtggt gctggcggga atgcgtcgat aagtctttga
     5161 tgacacgcgt gagccgatcc tccatgaaca ggccgaggct caaatccagg tcaagccgcg
     5221 tggggtgcac gtgggcttgg ctcggatctc gggcttgatg acgacctctt gatgttagtc
     5281 ttttgcgacg agctgaacac cggcagtatc gacggcgcga acgccgggga cctgccttgc
     5341 ggcctctgcc gcttcgagcg cgatgacttg aaatcgcgtt ttgccgctca gatatacgat
     5401 cccgttctca gatttgacgt cgaatccaac ggccttcagg gtttcgctgc gcgacaggcg
     5461 atcccgaacg acgtgcgcaa tggtctcgtc ttgcttttta gccagtttat cccgaagcgc
     5521 cggcgtcacg ttcaccttgt ttacgactcc ctctacaccg tcgaccttgg cggcgatctc
     5581 ggccactcgt gctttatcct cttcagtcgg gacggcaccg gttaacacgg ctttgctttt
     5641 atccatttcc acttcaattt cgaggggaaa caactgcgga tcggccatca gcgccaattt
     5701 gatggccagg ttcagcgacg tgacaggctt cttgtgctcc tctggttttg ggggtgacgg
     5761 ttcttctgcc tttttctcgt ccggtttctt ctcgtccgct ttcttgtcct cagccttctt
     5821 gtcctcggcc ttcttctcct cggccttctt ctcctcggct ttcttctctt ctactttttt
     5881 gtcgtcaact ttcttgtcgt ctgctttttt ctcgtccgat ttcctgtcgt ccgctttttt
     5941 ttcctcggct ccggcctttt cagacccggg ctttccatta ccgtcggact tgagctctcc
     6001 ctctgatttt ttcttcggtt cggccgtctt gggagtgtcc ttcgcccgtt cgcctccggt
     6061 gtcctcagcc gcatagcccg ttgcactgat cagagccaat actagcaatc cagagagggg
     6121 ccagatgaac atgtgactga ccttgccaac ctgtcgctca gcctgtacac actcgggaaa
     6181 acccatgagt ccgttgcatc ttcgctggta gacccggtga agcgtactgg ggaattcccc
     6241 agtcagccac ccccctactg aaaaacagca caaccgctgg aaacaggggc atctcctagt
     6301 taggtggtcc ccctgcaagt tggtatcgtt cgctgttgtc atccagactt cgaacgagac
     6361 agaagggaag gaaccatttg aagccgaaac ggattttggg aatcatcatg gcgggaggga
     6421 agggggagcg tctcctgcca ttgacggagg ttcgcagcaa gccggccgtg cccttcggtg
     6481 gcaaataccg catcatcgat tttgtgctca gtaattttgt caattcggga atcacatccc
     6541 tgtacgttct cgtacaatac cggtctcagt cgcttattga acatctccgt cacagctggc
     6601 agatcggggg gcgtctgacc caaagcttcg tgagcgtcgt ccctccgcaa atgcgggcaa
     6661 gggaaggatg gtacgagggc accgcagatg cggtgtacca aaatctcaac ctcatcgacg
     6721 actgggcccc cgacgtcatt gccgtgtttg gcgcggatca tgtgtatcgt atggacatcc
     6781 agcagatggt ccaagagcat ctcgagcacg atgccgacgc gacagtcgcc gccttgcctg
     6841 tgccgatcga agcggccggc gggttcggga tcctggaagc cgatgaagcg ggacgaatgg
     6901 tcggatttga ggaaaagccg acggcgccga agcccatgcc ggcaaggcag ggatacgctc
     6961 tgtcatcaat ggggaactat ctgttcaaga ccgatacgtt ggttccattg ctgaagcgcg
     7021 atgcctcgcg accaggcccg catgactttg gccgcaatat cgttccggaa ttgctggaag
     7081 accatcgggt ctacgtctac aaccttcaca acaatgagat tccggggttg cgctcgtatg
     7141 aagaacaggg ttactggcgg gacgtgggga ctctggaagc ttattggcaa gcgaacatgg
     7201 atgttctggg cgaacatcct ctcttcgacc tgcacaatac ggactggccg attttctcga
     7261 gcgggtatga cggtccgatg gccgctctgg tccggactca cgccgatgat tcgatgatcg
     7321 ggccgggtag tcactgcgtg gacgcggaca tccggaagtc aatcattggc cgcggagtgc
     7381 ggatcgaaca gggtgcctac ctggaagaat gcgtcattct ggatgacgtt catataggtc
     7441 ctcacgcgcg gttgcggcgg gtgatcgcag accggcgcag cacagtgccg gcctacagcc
     7501 gcatcggcta tgacgcggga cacgaccgca agcggttcca tgtgtcgcat tcaggtctcg
     7561 tggtccttcg gcatccttcg tccaccgatc ctcacatgtt gagacgtgcg tcatgactgc
     7621 tgccactctg agttccatcc ccctggccac ctaccggctg caattgcatc acggattcac
     7681 ctttgcggat gcgcaacggg ccgtgcccta cttgcatgcc ttgggaatca ccgattgcta
     7741 tttgtctcca atcagcaaag ccacgcccgg tagcgaccat ggatacgacg tcattgatcc
     7801 cgtccttttg aatcccgagc ttggaagcga agaggacttt caagaatttg tccggacggt
     7861 ccaagaacac ggcctgggga tcgtgctgga tgtcgtgccc aaccacatgg gaatcggcaa
     7921 gacgctgaac cggtggtgga gggatgtctt ggagaacgga ccaagctcac ggtatgcgac
     7981 ggccttcgac atcgattggc atcccatcaa acgggaactg gaaaacaagg tcttgctgcc
     8041 gattttagcc gaccaatacg gcgcagtcct tgagagccag gaaatcgaac tgacgtacga
     8101 agacggcgta tttttcttgc gctactacga ccacaacctg ccgctggcga cgaagacctg
     8161 gacccacatt ctctctcacc ggctcgatct cctggtcgcc gagggggagc aggcgatgcc
     8221 cgtgttggaa cttcaaagca ttctgaccgc gttgaagaac ctcccgggtg ccgccgagcg
     8281 cgaccccgaa cggattacgg aacattatcg tgagaaagaa atcgtcaaga agcggctggc
     8341 cgcgctgatg gacggcagtc cggtcatccg caccttcgtc ctggagaacg tgcgcctctt
     8401 caacggggaa cggggacggt cggaaagctt cgatctcctc gatgcactgc tgaacgaaca
     8461 ggcctatcgc ctcgcatcct ggaaagtggc atctgaggaa atcaattacc gccggttctt
     8521 cgacatcaat gaactggccg ccatccgcat ggaggaggag gccgtctttc aggaatccca
     8581 ccaactggtg ctgcgcctgg tgcgggaggg aaaggtccgt ggctgccgca tcgaccacgt
     8641 ggacggcttg tacgatcctg gacagtacct tcgacacctg cgcgagtttt tacagccgca
     8701 gcctgacggg gctcaggccc cgctgtatct cgtggttgaa aagattctcg gcaaggatga
     8761 accgctgccg gaatcatggc ccgtgcaagg gaccacgggg tacgatttcc tcaatcaggt
     8821 caatggattg ttcgtcgccg gtgcgcacga gcatttcttt accgatgtct acgcgaaaac
     8881 catcgaccgc cgggaggact atgccgatct ggtctatggc tgcaagcggt tgatcatgcg
     8941 cgtctcgatg gcgagtgaga tcaacgtgct cggccaccag ctcaacggaa tttctgagcg
     9001 ggatcgacgg tcccgtgact ttaccttgaa caacctgacc cacgccatcc gggaaatcat
     9061 cgcctgtttt ccagtctacc ggacgtacgt gaccgaaagc acagagacgg tggcggaccg
     9121 cgaccgcgcc tacatccata tggcggtcgc acgtgcgaaa cgacggaatc cggccatcag
     9181 cgggcaggtg ttcgatttta tccgctcgat tctcctcaag cagcgcgagg cacgtaccaa
     9241 agaagatcag gacgaacaaa tgcgattcgt catgaaattt caacaaacca ccagccctgt
     9301 gacggcgaaa ggcgtcgagg atacggcgtt ctaccggtat catcgcctgg tctcgttgaa
     9361 tgaggtgggc ggggagccgg agcagttcgg cttctcggtg gaggatttcc ataaacggat
     9421 gtgggagcgc cagtcccgat ggccgcatgc cttgtctgcc tcctcgacac acgacacgaa
     9481 gcgcagcgaa gatgtgcgcg cgcgaatcaa cgtgctctcg gaatggcccc aagaatggaa
     9541 atcgaaggtc agccgatgga gccgcttgaa ccggccccac ttccgcgaga gtgaaggcga
     9601 gcgcatgccc gaccgcaatg acgagtacct cttctatcaa acgctgatcg gggcatggcc
     9661 gttggaagcg atgggcgatg aagcctacca ggaattttgt gggcgtatcc aacgatacat
     9721 ggaaaaggcc gtgcatgaag ccaaggtgca tacaagctgg atcaacccca gccctgcgta
     9781 cgaccagtcc atgcagacct tcgtcgaggc tgtcttggaa cgaagcacct caaaccgctt
     9841 tctggaagac tttctgccct ttcaagagcg agtggcgcga tatggtctct acaactctct
     9901 aggacagttg ctggtaaaaa tgactgcgcc cggcatcccg gacttctatc aggggaccga
     9961 actctggtcc tttcacctcg tggatcccga caaccggtgt gccgtggact acgatgcccg
    10021 tgccgccatg gcggaggaac tgcgagcaac cctcaagcaa ttggggtctg accgcgcgga
    10081 gttcgccaga agcctgttcg agcaggcgca ggatggccgg gtgaagatgt acaccatcat
    10141 gaccggcctg gagtatcgcc ggcttcatcc cgaactgttc caacaggggg agtacctgca
    10201 actggagtgc ggtggcagta agaaacaaca cctctgtgcc tttgcccgtc tctatcaaga
    10261 tcacgctctt gtcacggtca ttccgcggct ggtggcgacc ttgaacccgg acagtaagca
    10321 tccgccgatc ggtctgccgg tgtgggagga cagttggatg gtcgtccccc cgtggccgac
    10381 gctgagtcga ttccgtaatc tcctcacggg cgaaatcatt acggcggaat cggtcaatgg
    10441 ccggcgtgtg cttcccctcg cgaaagtgtt acaccacgcg ccgatcgcct tactcgaacg
    10501 gctcccataa gggctaacga tagaagtgtt ttgggcgatg ctgggctcta gggctcatcc
    10561 cagttggtgg gtcaccagtg catggtatcg tcaagagaac aaagccactt gatgtgacac
    10621 aggcgctttg ctgacacgag gtgtttgcgt atgctcgaac cggaatatcg atcaggcgcg
    10681 acgaccatcg ccgaggaatc cgttgagctt gatcaacctt ccgtctcacc cgtggggtgg
    10741 cggcagtggt ctactcgcat tgaatcccag attgagcgct atccctggcc cacgattctt
    10801 ctggccttgg ggtgcggcta tcttctcgct cggcgattga
//
LOCUS       DASSCQ010000130         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_47059, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000130 DASSCQ010000000
VERSION     DASSCQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47059"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..568
                     /locus_tag="VFH05_09055"
     CDS             344..568
                     /locus_tag="VFH05_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678406.1"
                     /translation="MTRILVFLSMGLLASPTLAFAVEHNAGYRGIGYIYFSFIAAVLI
                     YGVNDAFGKTAMYVMAPIILVGLFLMLPPV"
     gene            complement(607..1485)
                     /locus_tag="VFH05_09060"
     CDS             complement(607..1485)
                     /locus_tag="VFH05_09060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6678407.1"
                     /translation="MKHHDQTSRPSAEPFSPPGMTRRGFVTATLAAGFAFAVRPVSAA
                     TITTDETGLTTGEIRIRTADGHIPAYRAKPKKGSAFPVVLVVQEIFGVHEHIKDICRR
                     LAKLGYVAIAPELYARQGDVLKMTDIDEIRKVVSKVPDAQVLADLDATVAWIKESGEG
                     NVEQLAITGFCWGGRIVWLYAAHHPTLKAGVAWYGRLVGTPSALQPTHPIDVASSLKV
                     PVLGLYGAEDQGIPLDTVEQMKTALRAADSPSEIVIYPNAGHAFYADYRPSYRKDAAE
                     DGWTRLQEWLKRHGVS"
     gene            1778..2842
                     /locus_tag="VFH05_09065"
     CDS             1778..2842
                     /locus_tag="VFH05_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET6678408.1"
                     /translation="MLRSTRAVSQSLFIPADLRPGRMLLKRIAILLTLVALVLLMLWV
                     ERDGLKDHHHDGELSFTDVLYFAMVTITTVGYGDIVPITPRARLLDALVVTPVRVIIW
                     LLFLGTAYQLVVRQYMEGYRMGKVQAALNDHTLVCGFGYTGWSAAKELLAKGVAAEQI
                     VVIDPNEDRIRAALELGLVAFRADATQETTLEHAMIQCARAVIISAGRDDSNALILLT
                     ARHLNPTVEILVSAVEEENVKLFRQGGATGIISPSTFGGYLLAAAVGHPHFTEYVQDL
                     LTAGGRVTLTERQPKPEEIGQSAAALKPDILLRLYRRGEVYNFWDLQEGQNMEAGDTL
                     LLLTSAVRTSASRQDHSQAA"
     gene            complement(3342..3644)
                     /locus_tag="VFH05_09070"
     CDS             complement(3342..3644)
                     /locus_tag="VFH05_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678409.1"
                     /translation="MNADMYKSLQRSWALTATRRAVDKKGLAVALTFLVAMLFGVREC
                     SDRTTGPVTNALQQPDLSTEIEIRSAGRSPREAEFSGPGQSMTGVRRELDDPQGSR"
BASE COUNT          740 a         1124 c         1090 g          814 t
ORIGIN      
        1 caggccatcc aagtgccata cctatctggc ccgaatcgct agctgaaaca tcgatccctt
       61 tcccttattt aattacggaa gagcgtcgaa ttatgagcat acgattaatg ctcgactcgc
      121 taggaaaccc gtgaggaagc aatgaatccg atccggccct gaatcgccgg agtatgcggg
      181 cagcccggcc atcatttgac ttcctctagc gggaagcgca gcatgaggct ctcttcctca
      241 agaaagaggt gttctggtgc ctcccaccgg cagcgcgttg tgctatttgc tcccgggaga
      301 acagatcacg tcttttactc tcacctcaac caaggagtct gccatgacac ggattctcgt
      361 gttcttatcc atgggactac tcgcctctcc aacgctcgcc ttcgccgttg agcataacgc
      421 cggctatcga ggaatcgggt acatctattt cagcttcatt gccgccgtgt tgatctatgg
      481 tgtgaacgat gccttcggca aaacggccat gtatgtgatg gcaccgatca tcctggtggg
      541 cctctttctg atgttgccgc ccgtgtaagt ttgtagagtc ggggacaaga aaaggggctg
      601 aaccgactac gaaaccccgt gacgtttcaa ccactcctgt agtcgtgtcc atccgtcctc
      661 ggccgcgtct tttctgtagc tcggtcgata gtccgcatag aacgcatggc cggcgttcgg
      721 atagattacg atctccgacg gactgtccgc tgctcgaagc gccgtcttca tctgttcgac
      781 cgtgtcgagt ggaatgcctt gatcctctgc cccgtagagt cccaggaccg ggactttgag
      841 cgacgaggcc acatcgatcg gatgtgtcgg ttgaagcgcg ctcggtgtcc cgacgaggcg
      901 cccgtaccag gccacacccg ccttgagcgt cggatgatgc gccgcataca gccagacgat
      961 acgtcctccc caacaaaagc ctgtgatggc gagttgctcg acgttccctt ctcctgactc
     1021 cttgatccag gccactgtgg catcgagatc ggccaagacc tgcgcatcgg ggactttcga
     1081 gacgactttg cggatctcat cgatgtcggt catcttcaag acatcaccct gacgagcgta
     1141 gagttcgggc gcgatggcca cgtagcccaa tttggccagc cggcggcaaa tgtccttgat
     1201 gtgctcgtgc acgccaaaga tctcctgaac caccaacaca accgggaatg cgctgccctt
     1261 cttcggcttt gctcgatagg cggggatgtg gccatcagcg gtccgaatcc ggatctcgcc
     1321 ggtcgtcagc cctgtctcgt ccgtcgtgat ggttgcggcg gagaccggcc gcacagcgaa
     1381 tgcaaagccg gccgccagcg tcgcggtgac gaatccacgg cgcgtcatgc cgggagggga
     1441 gaagggttcg gctgacggtc tcgacgtctg gtcgtgatgt ttcatggcat tctaccccgc
     1501 taaaaagatg gactgatgtc tggattatca caagagaccg tgcgccgtcc tgccctcttc
     1561 attcccgccc gatgcagcca cagccggtgg atgccgctgt gcccgaggcc ctcccacgag
     1621 aagaccatgg ccacaagcac gcacgcgcgg gccagacaat aaacccagat gaccatgctc
     1681 cccaacacac gggactcgga aagatcctag gaggggggct gcccagaaac cggtagtctc
     1741 gagtaagtag tatctgttct ggatagcttg gtgtgtgatg ctccgctcga cccgcgctgt
     1801 cagccaatct cttttcatcc ccgcggacct gcggcccggg cggatgctgc tcaagcgcat
     1861 cgctatccta ttgacgctgg tcgcactcgt gctgctgatg ctctgggtgg agcgggacgg
     1921 gctgaaggat catcatcacg atggggagct gtccttcacc gacgtcttgt acttcgccat
     1981 ggtcacaatc acgactgtcg gctacggcga tattgttccc atcacgccac gcgcgcgcct
     2041 gctcgacgcg ctggtcgtaa cgccggtgcg ggttatcatc tggctgctgt tcctcggtac
     2101 ggcctatcaa cttgtggtcc gccagtacat ggagggctat cgtatgggaa aagtgcaagc
     2161 tgcgctcaac gatcacacgc ttgtctgcgg atttggctac accggctggt cggcggccaa
     2221 agaactactg gccaaaggag tcgccgcgga gcaaatcgtc gttatcgacc cgaacgaaga
     2281 tcgcatccgc gctgcgctag agctaggcct ggttgcgttc cgggccgatg ccacgcagga
     2341 gaccacgctc gagcacgcca tgatccagtg tgcccgggcc gtcatcatct cggccggacg
     2401 cgacgacagc aatgcgctga tcctcttgac tgcccgtcat ctgaatccga cagtggaaat
     2461 tcttgtcagc gcggtggagg aggagaacgt caaattgttt cgacagggcg gcgccacggg
     2521 cattatttcg ccgtcaacct tcggcgggta ccttctcgcc gccgctgtgg gtcatccgca
     2581 tttcactgaa tacgtgcagg atctgctcac cgcgggtgga cgggtcacgc tcacggaacg
     2641 acagccaaag ccggaggaga ttgggcagag cgctgcggcg ttgaagcccg atattctgct
     2701 gcgcctgtat cggcggggag aggtctataa cttctgggat ctgcaggaag gccagaacat
     2761 ggaggcgggc gacacccttc tcctcttaac ttcggccgtc cgcacgtccg catcgcgcca
     2821 ggaccattcc caggcggcct aggtttcgcc caccttgagt gaacctattc tacgtccagc
     2881 gcgtcatctg ggtgactatt ggatgcccca ggcgagtaac acgcgccagc ggccccgaga
     2941 tcgtagccgc cgatcaggtc gtgggagatc accggggagc acgtgggggg aagtctttcc
     3001 ttggagccgg gtcgatggtt acaacctggg gcatcgaaga aaacaacggt gagccgacag
     3061 cagactttcc gggagcattg gttcgctact tggctacaag atgtattgat tgggtccgta
     3121 ttgtggtttc ggcgtcattc gttgcatcgc ccaggccgtt ttcattcacg tggtccctgg
     3181 tagtgcgatg gtgacgatag gccttcatgg ccgcttggac cacatgcacc aggaacgtac
     3241 tttcgggttc ggcagtagta ccctcaatgg ttacagtgaa cgacgacatc cagacctcct
     3301 tagttcagaa agtgtttgag aacattgtgg acctcatggc gtcaccgcga cccctgcggg
     3361 tcgtccagtt ctcgcctgac ccctgtcatc gattgaccgg gccctgaaaa ctccgcttcg
     3421 cgaggactcc tgcccgccga acggatttcg atttcagtgg agagatccgg ctgctgcaag
     3481 gcgttggtga ccggcccggt cgtcctgtcg ctgcattctc gcaccccgaa gagcatggcg
     3541 acgagaaatg tcagagcaac tgccagacct ttcttatcca cggcccgtct cgtggccgtc
     3601 aacgcccaac tacgctgtag cgatttatac atgtctgcgt tcatggtggt ccttgcagaa
     3661 tcttctagtc ctaccggcac aatcgccgcc gatacaccgc tcccagccgc aaggcgtctt
     3721 gaaggaaacg ttggcttaga ggagtgtagg agggaagagc gatacggg
//
LOCUS       DASSCQ010000131         8260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_925498, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000131 DASSCQ010000000
VERSION     DASSCQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925498"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2392
                     /locus_tag="VFH05_09075"
     CDS             <1..2392
                     /locus_tag="VFH05_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678410.1"
                     /translation="QFDNQSSDGVITGFSYEQSVRPFTMLEKKVKKGLPLPMNTTFTK
                     AAKKGERIITVKHAAQYHPNVELLIGADNVKGNEIGRVKSIKGNEITLYRPLKNDHPV
                     KDIVTVEFVRQRFWVDSDVGTVFWHDHALGRVTWPHGGFGTIVIEPVGSTYHDPKTGK
                     QVRSGPLVDIRTAEPVGFGVNGSFRELVVQLNDTVPHTVNIVTEGNPPGQPIEVALEA
                     GKTVSFPMPEKIPMSPMAFLNGGTHTTGGGLNFKVEPISSRLAVNPDTSKLFSSEVHG
                     DPYTPMVRAYLGDTVVFRLLQTMANETMVWTLSGHTYLTERYAGDANRKNSIHIGIAE
                     RYDLVVPQAGGPRLQPGDYIHLNGRVSKFSEGAWGIMRVLDKEVPDLQKLPAGYSRRN
                     EIPKPLGVCPADAPVKAFNVVAMDYPGMKLNPNAPDVIEVDFERTIKMVNPEAKIYTL
                     EEEASKVASGIQPMPLTLRANVGDCIKVKLTNRMKEGRASFSAISLAFDPKDSLGANV
                     GNNPGDQTVGPGENRTYTYYADPFIGETASLVWDWGNIVVNPRNGLYGALVIGPKGAK
                     YRDPKTGADISMKNSWVADVIVDRTIQGYEHRTNYRDVALFFQDEDNIIGTSFMPYVQ
                     NTAGLTAVNYRAEPYKFREEKGCTLGKVFQPCVVDKPEDPATPIIEAHAGDPVRIHVF
                     GASNEQNGMFSVERHEWPIEPFMRGADMISVVEFSGSETLDAFIPTAGGPFRMPGDYV
                     WSNQRLPYAQSGQWGYLRVLPPGDNRIKGLTDARSGSQQAGVEGEGRGDVLPVSMK"
     gene            3109..3723
                     /locus_tag="VFH05_09080"
     CDS             3109..3723
                     /locus_tag="VFH05_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6678411.1"
                     /translation="MREQQALVVDDHPIIRDGIKQLLVRAFPALHIRDSSGTDRVLEE
                     ICSYPWAFVTLDINLPGQNGLHIIREIQGCCPKVPIIVFSLFSEDQYGARALRAGAAA
                     YLSKERSPLELVEVVRSVLRGEKKGQSQFAAPALSIRETEVLTLLGKGMNRQEIAHEL
                     GINEKTVSTYRARLLHKLNARTTVNLLRFAADEGLLGADQTPIG"
     gene            4486..5493
                     /locus_tag="VFH05_09085"
     CDS             4486..5493
                     /locus_tag="VFH05_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678412.1"
                     /translation="MMSWTRSARRGFFIGVIGTAAFWQPGGIGAQVPMEPQPGPPESG
                     TVQQGTTQTAPTTQAPGGTVQQGTTETAPTTQTPPTQTPRTDQAIAEQQRRAELEREV
                     RDARRYRDEHRHHGETYVAGFGGVTFGGTTRNVEGRGSALGASIDNPTLSDSAVYGVK
                     IGYFHPGRLNWLGLELEGFNTTPHIMQSGGQPGTHLRLTTLGLNVIARKKLACRDRGD
                     RREGDIRKSDAWEIDDYSALHDNARCPLQVYAGVGLGVFFAETSNQFGRATDNARAGL
                     NALAGTKFFFNEHIALFAEYKFNYVDLRFDQNQVLGGATAGLNGTYLINHVVGGLAFH
                     F"
     gene            5758..6378
                     /locus_tag="VFH05_09090"
     CDS             5758..6378
                     /locus_tag="VFH05_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678413.1"
                     /translation="MTVRAMTQPFDDPEWLYEIKHDGFRALTVIEDGRCRFYSKHKHR
                     LTGFRELAESIMRELRLDNAILDGELGASDELGRTVFTALMQRTKPARYFAFDLLWLN
                     GRDLRSLPLQWRKTELKRLLPARSADILYVDHTKGAGTDLYRLACQLDLEGIVAKRAD
                     SQYGEIEMARDWIKINNPYYSHHERRGEFFRKVEARRAAKDRGLRP"
     gene            6861..>8260
                     /locus_tag="VFH05_09095"
     CDS             6861..>8260
                     /locus_tag="VFH05_09095"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HET6678414.1"
                     /translation="MQGRGAAYGAVIVLLTLGIRMALAQRKQAEAQLKAIAWLLREGG
                     DPFTVPEPQPYGDLSELNTARTILRTVGKELLVNVAGDALHLIGTSGAVCERNGDYAS
                     CLVASDWCRFLDSTSRERCRTADNGAALASGEWHCRNSCRDVSARSMETGEPVDLPCN
                     GGINIYAAPIKVDGETVGSISIGYGDPPKDEAQLIEVAERYGVRAEQLRELSAGMPSR
                     PQFIVAVAKARLRSAALLLGEIIKRKRAEEALADLTKNLEARITERTEQLRALATELN
                     LAEQQERKRLATELHDHLQHLLVLAKLKVGQGKRYAEPIPALIELLKQLDDALSQALR
                     YTRTLVAELSPPVLRDYGLAAALKWLSEYMQKLSMTVMVHVPESSELTLPEDQAVLLF
                     QSVRELLINCYKYAGTSRATVLLEQREGQLRIQVRDEGVGFDPTAATPAEIPAGGPSS
                     KFGLFSIRERMTALGGS"
BASE COUNT         1919 a         2263 c         2358 g         1720 t
ORIGIN      
        1 gcagttcgac aatcagtcat cggatggcgt aatcaccggg ttctcttatg agcaatccgt
       61 acgccccttc accatgctgg aaaagaaagt gaaaaaaggt ctgccgttgc cgatgaacac
      121 cacgttcacg aaggcggcca aaaaaggcga gcggatcatc accgtgaaac atgccgccca
      181 ataccatccg aacgtggagc tcctcatcgg ggccgataat gtcaaaggca acgaaatcgg
      241 ccgcgtcaag tccatcaagg gcaatgaaat cacgctgtac cggccgctca agaatgatca
      301 ccccgtgaag gacatcgtga cggtcgaatt cgttcgtcaa cggttctggg tggactcaga
      361 tgtcgggacg gtgttctggc acgatcatgc gcttgggcgc gtgacatggc cgcacggtgg
      421 tttcggcacg atcgtgatcg agcctgtcgg ctcgacgtac cacgatccca aaaccggcaa
      481 gcaagtcagg agcgggccac tcgtcgacat ccggaccgca gagccggtcg gatttggcgt
      541 gaacggcagt ttccgcgagt tggtcgtcca gctcaacgat accgtgccgc ataccgtgaa
      601 cattgtcacg gagggcaacc cacccggaca gccgattgaa gttgcgctcg aggcggggaa
      661 gacggtgtcg ttccccatgc cggaaaagat tccgatgtcg ccgatggcct ttctaaacgg
      721 agggacccat accaccggag gcgggttgaa cttcaaggtc gagccgatct cgagccgcct
      781 ggcggtcaac ccggatacgt ccaagttgtt cagcagcgag gtgcacggcg atccctacac
      841 gccgatggtg cgcgcctatt tgggcgacac cgtcgtgttc cgtctgcttc agacaatggc
      901 caatgagacg atggtctgga cgctgtcggg ccatacctat ctcaccgagc ggtatgccgg
      961 cgatgccaat cggaagaatt ccatccacat cggcatagcc gaacggtatg accttgtcgt
     1021 gccgcaggca gggggcccgc ggctgcagcc cggcgactac atccatttga acggtcgggt
     1081 gtcgaagttc tcggagggtg cttggggcat catgcgtgtg ttggacaagg aggtgccgga
     1141 ccttcagaag ttgccggcag gctatagccg tcgaaacgaa attccgaaac cgcttggggt
     1201 ctgccccgcc gatgctccgg tgaaggcctt caacgtggtc gcgatggatt atccggggat
     1261 gaaattgaat ccaaacgcgc cggatgtcat tgaagtggac ttcgaacgaa ccatcaagat
     1321 ggtgaacccg gaggcgaaga tctacacctt ggaggaagag gcctccaagg tggcaagcgg
     1381 aattcaaccg atgccgctca cgttgcgtgc gaacgtcggt gattgcatca aggtcaagct
     1441 gacgaaccgc atgaaagagg gccgggcttc gttctctgcc atcagcctgg cttttgaccc
     1501 caaggattct ttgggggcga atgtcggtaa caatcctggc gatcagactg ttggcccggg
     1561 ggagaatagg acctatacct attacgcaga cccgttcatt ggcgagacgg cctcactcgt
     1621 ctgggattgg ggcaatattg tggtgaaccc gaggaacggc ctctatggag cgctggtcat
     1681 cggccccaag ggtgccaaat accgtgaccc gaagacgggc gccgacattt ccatgaagaa
     1741 ctcatgggtg gcggatgtca tcgtggatcg tacaattcag gggtacgagc atcgaaccaa
     1801 ctaccgtgac gtcgctctgt tcttccagga cgaggacaac atcattggga cgagtttcat
     1861 gccctatgtg caaaataccg ccggtcttac agcggtgaat tatcgggcgg aaccgtacaa
     1921 gttccgggag gaaaaaggtt gtactctcgg caaggtgttc caaccctgcg tcgtggacaa
     1981 accggaagat ccggctacac ccattatcga ggcgcacgcg ggcgatccgg tccgcatcca
     2041 cgtattcggt gcgagcaacg agcaaaacgg gatgttcagc gtcgaacgcc atgaatggcc
     2101 gatcgagccg ttcatgcggg gggccgacat gatcagcgtg gtggagttct cgggatcaga
     2161 aaccttggat gcgttcattc ccactgctgg cggcccattc cgcatgccag gcgactatgt
     2221 gtggtcgaac caacgcttgc cctatgccca gtcgggccag tggggctatc tgcgggtttt
     2281 gcctccgggc gacaaccgca tcaagggcct gacagatgcc cgctccggca gccagcaggc
     2341 cggcgttgaa ggggagggtc gtggagatgt ccttccagtg tcgatgaaat agccttcatc
     2401 aggcgaggga ggtggcactg ccgcttccct cgccctcaac tatcattatc ccgtcgtggc
     2461 tcgagctgct caccgtccct tgtctgccgc ccagtggaat ccctagatgt catgcaatcg
     2521 cacaagggtg taggctgaaa ctgatagaca aaacgccctc aggatcaata ttctctaact
     2581 cttatggctc aagagaacgc caagcacttt gaacgctgcc aaaaatgcca gggtagcctt
     2641 cgggcagaga ctggtattga tctcgtaaca gggaccacta ttctccagta cgtctgcttc
     2701 aactgtggcc gcaggtggca tactgaagag cccaagccgt tggcggccta aagcgcactt
     2761 atagaaccca gcatcgacag caatcggcac caatacttac cttgcctccc gccggtcctc
     2821 ttgaccccca tcgtccgtct cgtctgagaa aaagagattt gtaggtcatc gctgatagac
     2881 aacacgcgca ttttggcgga tagtcgggca atttgaatgt catccaccat gcaaaatgaa
     2941 tcggacacct tagccacgtt tggccagtgt agtgtccagg tagaccgggt gttgaaacag
     3001 ggacctcatt gactcaggag gagtatctgt ccatcgagaa ctgtctcctg gtcgttcaac
     3061 ttgcgttagc ctcctcgagg ctcgcgtcca aaaaacgagt acctcgtcat gcgtgagcaa
     3121 caagccctcg tcgtggatga tcatccgatc attcgagatg gcattaagca gttgcttgtg
     3181 cgagcgtttc cagcgttgca cattcgtgat tcttcaggga ctgatcgggt cctggaagag
     3241 atctgtagct acccttgggc gtttgtcacg ctggatatca atctcccggg tcagaatggt
     3301 cttcatatta tcagggagat acaaggttgt tgcccgaagg tccccattat tgtcttcagt
     3361 ctattttcag aagaccaata cggtgcgcgc gccttacggg caggggccgc cgcatacttg
     3421 tcaaaggagc gctcaccgtt ggagttagta gaggtcgttc ggtctgtcct gcggggagaa
     3481 aaaaaaggtc agagtcagtt tgctgctcca gcgttatcca ttcgagaaac tgaggtcctt
     3541 acactcctcg gcaaagggat gaatcgacag gaaatcgctc atgaacttgg aattaatgag
     3601 aaaaccgtga gcacatacag ggccagactg ttgcacaagc tcaacgctcg cacgacggtc
     3661 aatctcctgc ggtttgcggc tgacgaaggg ttgcttggtg ccgaccagac gcccataggc
     3721 tgagcgacgc ctgactcgtc gcttctcaag aagcccattg acgagcctgg tggattccct
     3781 gagggcacaa tcgtacaaaa aatctgacga atcctgtggc tatgacaaga agcatgatga
     3841 gtaaaataac gggtcatact tgaggcccca tggaatcgcc ggagctgaga aagttttata
     3901 ttgccatcca gaaccttcgt gaagcgatga cacacgatct gccgcttgat gaatttgacc
     3961 aaatttgcct cgaaaactac atagcgctac tccagatcac gtacatggag tggaagcggc
     4021 gcaacatcca acagcccgcc cacagacgcg cagcgtaacg ctggccctgc attccgcgat
     4081 cctgcaagaa aacaacacgt cttttagacg cgtggcggct taccgtcttg ctgaagcgtg
     4141 ggacgctcga ctccaccggg taccctcgtt cggaagccta gtaccacggc ggtttcaaag
     4201 aagtgagtcg cacgattgaa ttccgtacat cacagcgcct cccacttcgt ctttctcatc
     4261 acatcgcccg agtaaaatct aggcagcgcc ggataaaccg tctttcgtcg gacactctat
     4321 ctacagcccg actgagtgga acctgggaca ttgctcgggc gtgtctagct atttctcacc
     4381 ggtcccctag aggtgcttcg cacaaaacca ggttggtcca ccgcatcgtc tcaatggaat
     4441 tgctcttgca cattagatga gcacaacttg agcaaaggag attgcatgat gtcatggaca
     4501 agaagtgcga ggcgggggtt tttcatcgga gtgattggca cggcagcgtt ttggcagccg
     4561 ggagggatag gcgcgcaggt ccctatggag ccgcagccag ggcctcccga gagcgggact
     4621 gtccagcaag gaacgaccca gaccgcgccg acgacacagg caccgggcgg gaccgttcag
     4681 caaggaacga cagagaccgc tccgacgaca cagactcctc cgacacagac acctcgcacc
     4741 gatcaggcca tcgccgagca gcaacgccgt gcggaactag aacgagaggt tcgtgacgcc
     4801 cgtcgttatc gggatgaaca tcgacatcat ggtgaaacgt atgttgctgg gttcggcggt
     4861 gtgacattcg gtggcacgac gagaaacgtc gaagggcggg gtagtgcgct gggagcgtca
     4921 attgacaatc cgaccctctc cgattccgcc gtgtatggag tcaagatcgg ctattttcac
     4981 ccagggcgac tcaactggct cggcctggag ttggaaggct tcaatacgac gcctcacatc
     5041 atgcaaagcg gtgggcagcc cggcactcat ttacgcctta cgacgttagg gctcaacgtg
     5101 atcgcacgga agaagcttgc ctgtcgcgat cgaggcgatc gtcgggaagg cgatattcga
     5161 aagtccgatg cctgggagat cgatgattac tcagcgctcc atgataatgc acgctgtcct
     5221 ttgcaagtat atgcaggtgt agggttgggc gtgttttttg ctgaaacttc taatcaattc
     5281 ggccgtgcga cggacaatgc gcgtgcgggc cttaatgccc tggctggaac aaagtttttc
     5341 tttaacgagc acattgccct gtttgccgag tacaaattca actatgtcga cttgcgattc
     5401 gatcaaaatc aggtcctagg aggtgcaaca gccggtctaa acgggaccta tttgatcaat
     5461 catgtggtgg gtggactggc gtttcacttc tgagggatca aacatcttag aacccgcagg
     5521 agtctgcggc ggtgatgaaa gccccacata gccgcaacgg tctctaccct ccttgagcac
     5581 cagctctctg cgggaatagc gccggttatt aggaccggcg ctacctattt tgagcgcaga
     5641 gttctcgacc agcctagccg cttcgaacac aaacgagata agctactttt aagcccttcc
     5701 taatactatc ctaagagagc ctcggtatct tgtcagttga tggtgattcc gacgccgatg
     5761 acagtacgtg ccatgacgca gccgttcgat gacccagaat ggctctatga aatcaaacac
     5821 gacggatttc gtgcattgac agtaatcgag gacggacgat gccgctttta ctccaagcac
     5881 aaacaccgac tcaccggttt ccgagaactt gcagaatcca tcatgagaga acttcgcctg
     5941 gacaatgcca ttcttgatgg ggaactggga gcgagcgatg aattgggccg aacggtgttt
     6001 acggcactga tgcaacgtac gaagccggcc cgatactttg cctttgactt gctctggttg
     6061 aacggccgtg atcttcggtc gctgccgctc caatggcgca aaacagaact gaaacgtctc
     6121 cttccagctc gatccgctga catactgtac gtcgatcaca ccaaaggagc tgggacggac
     6181 ctctaccggc tcgcctgtca gttagacttg gagggcattg ttgccaagag agctgacagt
     6241 caatatggcg aaatcgaaat ggccagagat tggatcaaaa ttaacaatcc atactacagc
     6301 caccatgagc ggcgaggaga attttttagg aaggtcgagg ccagacgtgc ggccaaggac
     6361 agagggctcc gtccgtaacc agcatcagca ttcccattca agttttacct tcgagtccgg
     6421 gccagcacag agaatagatc cgaaatttga gcatccctat cggcgatacc ctgcgagaga
     6481 tcgtccgaaa ccgcgagttc atcacttaat cgagtgcagc ctcttccagc cgtcatctgc
     6541 tgaagctcct agtgtcaggt aaagaagaaa cgtaggtcct gcaatgacag gagcaactat
     6601 aggccaacaa ctgtcagact aagtccctat tggtttcgta ttcgcccacc tacaccctct
     6661 acaacagata gtcacttcaa ggcattcatc aacgtaaaat cagcaccttc tgatcgtcat
     6721 atgggccaat tctaggcatg aggattgccg agagggatct cggacacatg cgcctaccgg
     6781 cagatgtgag ttattcgatg cacgcaagca atgccctcat aaggctcgcc tcgtcgacaa
     6841 aggccttggt gccaaagcgt atgcaagggc gtggagctgc ttacggcgct gtcatcgttc
     6901 ttctgacgct tggcatccgc atggcgctag cgcaacggaa acaggcggaa gctcagctca
     6961 aggcgatcgc ctggctcctg cgtgaaggcg gagatccttt taccgtgccg gagccgcaac
     7021 cttacggaga cttatcggag ctcaacaccg cgcgcaccat tctccgtaca gtcggaaaag
     7081 aactcctggt caatgtcgcc ggtgacgcgt tgcacctgat cgggacgtca ggggctgtgt
     7141 gcgagagaaa cggagactat gcctcatgcc tcgtcgcgtc cgattggtgt cggtttctcg
     7201 acagcacgtc gcgagagcgg tgtcgtactg ccgacaacgg agctgccttg gcgagcggcg
     7261 aatggcattg ccgcaattca tgccgcgacg tctcggcccg ctcgatggag acgggcgaac
     7321 cggtcgatct accgtgcaat ggcggaatta atatctacgc cgctccgatc aaggttgacg
     7381 gagaaaccgt cggaagcatc agcattgggt acggggaccc gccgaaggac gaggcgcagt
     7441 tgattgaagt ggctgaacgc tatggggttc gggctgagca gcttcgtgaa ctcagtgccg
     7501 gtatgccgtc tcggccgcaa tttatcgtgg cggttgccaa ggcgcggttg cgtagcgccg
     7561 cccttctcct gggcgaaatt atcaagcgga agcgtgctga ggaagccttg gccgatttaa
     7621 cgaagaacct agaagcgcga atcaccgagc gcacggagca acttcgggcc ttggcgacgg
     7681 aactgaacct tgcggagcag caagagcgca agcgactggc cactgaactg cacgatcacc
     7741 tgcagcatct tctggtgctg gcaaagttga aggtcggtca agggaagcgg tatgccgagc
     7801 caatcccggc gctcatcgag ctcttgaaac agctcgacga cgccttatcc caggccctcc
     7861 ggtatacgcg gaccttggtc gcagaattga gtcctcccgt gctcagggac tacgggcttg
     7921 cggctgcgtt gaaatggctg agcgaataca tgcagaaact ctccatgacg gtcatggtgc
     7981 acgtaccgga atcaagcgag ctcacattgc cggaggacca agcggtgctg ctgtttcagt
     8041 ctgtgcgaga actcctgatt aattgctaca agtatgcggg cacctcccga gctaccgtcc
     8101 tactcgaaca gcgcgaaggg cagctgcgca tacaggtccg agatgagggg gtgggcttcg
     8161 atccgacggc cgccacgccc gccgagattc ccgccggagg tccgtcatcc aagtttgggc
     8221 tctttagtat ccgcgagcgc atgaccgcgt tgggtggatc
//
LOCUS       DASSCQ010000132        18824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1556140, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000132 DASSCQ010000000
VERSION     DASSCQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 18824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18824
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556140"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFH05_09100"
     CDS             complement(<1..477)
                     /locus_tag="VFH05_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET6678415.1"
                     /translation="MRWQGQRQSENVEDRRGFGPARIGGVGGLGLGGIVLVLAVSYFT
                     GMNPLTLIDLMSGIQSTTTEPPIESVPTGPVKDEIGQFASVVLADTEDTWQQLLPKAG
                     GHYENPRLVLFTGAVHSACGATSSAVGPFYCPQDRKLYLDVSFFKDMSQRLGAPGDF"
     gene            complement(598..1905)
                     /locus_tag="VFH05_09105"
     CDS             complement(598..1905)
                     /locus_tag="VFH05_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6678416.1"
                     /translation="MIPRRDCHHRILIQTSLIVSALLMGSGCVSTDTHQKALEELEKS
                     KKLSASQAAELDALKKKAKADSDQLQQQLAGLQHNLDEQTMQRKNAEQQAASLEKERE
                     ALTARSAELQNRLDGLEREKSQLGTELSDVRGQIATLEKKLADETAQVSALESDKQQL
                     ASGTATAQSEIIKLQQRAAELEAESARVAKEREQLRQEQSRLSAALDQERQRLKTETE
                     EKAKLAKEREQLQQEQSQLSASLDEERNRLKAEAEDKARLEQERAAKEDEIRRLTKTQ
                     EELSRSLKDEIAKGNITIQQVRDRLTINMVDRVLFDSGRADIKPAGVKVLKQVSDVLS
                     KVGDKQIRIEGHTDNVPISTKLQDKFKTNWELSTARATIVVRYLIDQGGVQREVLSAV
                     GYADTHPIASNDSEQGRSSNRRIEIVLYPKDLSQIAGGLPPAN"
     gene            complement(1902..2099)
                     /locus_tag="VFH05_09110"
     CDS             complement(1902..2099)
                     /locus_tag="VFH05_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678417.1"
                     /translation="MQGPLVLPTRIETVVSEASLIEDIEVIEYPAFVLAFPLHRLVLN
                     KGTTLLGPDSSSIYTFIQEGL"
     gene            complement(2225..2404)
                     /locus_tag="VFH05_09115"
     CDS             complement(2225..2404)
                     /locus_tag="VFH05_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678418.1"
                     /translation="MTRFAHLIVLAAFLALGVGAPVSFANEPAPAEQKEKKDSSGSKA
                     DDENKKDDKKGMGGK"
     gene            2733..3341
                     /locus_tag="VFH05_09120"
     CDS             2733..3341
                     /locus_tag="VFH05_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6678419.1"
                     /translation="MSRTIVFHKPYDVLPCFKDPDGRPTLADYITVPGVYAAGRLDRD
                     SEGLMILTSDGALVHRITDPQHKLPKVYWAQVERVPAEGALNQLRRGVMLRGIRTRPA
                     HVRLLRQEPLLWERPVPIRVRRSVPTAWLEVVIHEGKNRQVRRMTAAVGHPTLRLIRV
                     AIGAIRLDNLQPGQWRDASSAELRTLCHQPRLEAPAHASKRR"
     gene            complement(3813..4637)
                     /locus_tag="VFH05_09125"
     CDS             complement(3813..4637)
                     /locus_tag="VFH05_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678420.1"
                     /translation="MSERTFTDPLANEFYRQGASELESAQSAEAVLRKAEAFGQKDAR
                     TEIMQSAFYFLAAAHFLEGRNAGDSAQAYHQAAQQLHRIGHFTQAGRAYSNAGRLAEH
                     AAQAAASPSSRHDLQHLAVRSYSRANHCFAEVGELDWSQTEYLNERNARVTWAKMEGK
                     HPWAQLAWKFTSNYGTSFSRWGLWVVGTIGLFSLLYELFFHLRWLQPMETDAVAPWIP
                     FWSAVYYSVNVTAALGLVDYQPTHVISQGVVIVNVLAGYILLGIGIGIIGRMIRHR"
     gene            4804..5481
                     /locus_tag="VFH05_09130"
     CDS             4804..5481
                     /locus_tag="VFH05_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678421.1"
                     /translation="MKRLFYVLVMTVAFAGCAVEQEYSRTPRTAVEQLLLTQAIERSF
                     HNLRLELPPGATLELQATSLDSDRTLVRITGPELASAKHPALETTYIRDAVAAVLGRH
                     GYRIPNQDTNYLVRVMVESLGTMQGLTLIGLPPIQSTVIPFSLPELALFKFQKQSRYA
                     RLHVNMFDNRTGDSSVRPRRSSDGRIMISIRCCFISPGPQRICSLHHEAQERKGRPLW
                     LTAMSVA"
     gene            complement(5465..5617)
                     /locus_tag="VFH05_09135"
     CDS             complement(5465..5617)
                     /locus_tag="VFH05_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678422.1"
                     /translation="MIRKLSSGGYRLYSRKKDPKTGKRRNLGTFKTGAAAQKHEREVQ
                     YFKRHS"
     gene            complement(5689..6705)
                     /locus_tag="VFH05_09140"
     CDS             complement(5689..6705)
                     /locus_tag="VFH05_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6678423.1"
                     /translation="MRLSRRTLLQATGLGALAGMVGGCDAVGGVFGRMFAVPPRETTY
                     FTPNSKFYVVNYADSAVSVSREINIDQWKLTVKGDVKTPMSLGWRDILNRDSYDQVST
                     LMCIDTLPGGDSLGNATWRGISLKKLLQDAGADEDTARDVVFRGIDGYDDSIPFPRAM
                     QDDVMLAFLMNGEKLPKEHGFPLRAIVPGLYGIKNVKWIYEIEVYPGDYQGYWQRKGW
                     TDDGTIKIFSRIDSPGHYQTLRGDQQTFRGIAFGGPNSIGKVELSFDAGRTWNDCMIE
                     PPMSPYSWVLWSYTWQPPKPGKYQTVVRAIDTKGQLQIAELVRPQPAGASGYHTIISE
                     VSEV"
     gene            7006..7443
                     /locus_tag="VFH05_09145"
     CDS             7006..7443
                     /locus_tag="VFH05_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678424.1"
                     /translation="MNHPFRLRKSWIACPLIFMVCALVACAGGPSQSDPSWKSVPITD
                     VSMVVGEWDGTVTKDKAMFPEGAVRLIIRANSTYLFAGETLSKSAVGSGGVETREGRL
                     IGDTEHRAVTFTLYDHKGARMLVVDTTNKENGTQYHGQFTKVR"
     gene            7674..8321
                     /locus_tag="VFH05_09150"
     CDS             7674..8321
                     /locus_tag="VFH05_09150"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6678425.1"
                     /translation="MISCSNHEPSERSSQTYFTALQSGLILVLALNSCGEDRVTVLVE
                     AAEERMNPLSAADDHASEVRLHQALVDHFGFSALGLSTYVFMNRGYVVGYVDSLEQAQ
                     AVFETAKNVKGLQSVEAVLPVKQPPPTDVGKVISDSTLKSQIESALTRGTAMVSGRVH
                     VEVLNGRVVLLGVVSGSDERERAERAAAGTIGVTRVTNWLLLPETEYMAIRSQVF"
     gene            8331..9539
                     /locus_tag="VFH05_09155"
     CDS             8331..9539
                     /locus_tag="VFH05_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6678426.1"
                     /translation="MKPSCRLCLLVLVLAISPVSCAAARHGTTVPKPSGLPMQRVTVL
                     SYNTLHGLEPSGLIVKPSESNEARQARLDLQFEQLAVARPELILLQEVNPLPEMAETY
                     VAAMKRAGLEYSQVHQVDACGVRLAPGLAVVPHLNNGLVVLAKAPLRLRKVAGLKLSG
                     GVGRCVDYMGLQTGELRYALVAEVENPDTGKKLLAVSLHLHSGIERNAFFIQKINEAV
                     EQGRARREDFEGIVAAIEHDQNRRLDEIRLLVQEIQKLDARGQYIGTLVGGDFNFEPD
                     DPEYRELERAGLTDTYVMGNSQSEIYSLDPQKNVIAAKGVREVPSALREAMKRLPQSQ
                     QQKILEGYQTGISQARRVDFLFLMRKPSDGPTGCFHQELFGKPAMVSTQPGSDHYGVL
                     VTYLADASRC"
     gene            9585..9929
                     /locus_tag="VFH05_09160"
     CDS             9585..9929
                     /locus_tag="VFH05_09160"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HET6678427.1"
                     /translation="MLNMSMPLHELIGFVAGFGTTFAAMPDLISMLKRRSCVGMNPTM
                     AAIMGTFQIVWVYYGVLVESRPVIVWNTIAVGINFLVVGAYVYFACSRPRHAQVVNGS
                     HSSEGPRNSPPR"
     gene            9990..10559
                     /locus_tag="VFH05_09165"
     CDS             9990..10559
                     /locus_tag="VFH05_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6678428.1"
                     /translation="MRPGRRACLLILAMSLTACSSFKVNTDHDPTVDFSSFKTFAFAG
                     PSEANKGGIYDNSLTRKRIESAVVRELTGKGLRQVGLDEPQDLLVHYWVGIQDKQRLE
                     STGPTVGVARGPGGGYGWGAGYGGGVTTYEYKEGTLVLDLIEPPKKQLVWRATIVGTL
                     QDSPTDNVDLANKAIAKAFERYPPPVNTP"
     gene            10584..11273
                     /locus_tag="VFH05_09170"
     CDS             10584..11273
                     /locus_tag="VFH05_09170"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HET6678429.1"
                     /translation="MTKSATTTAALIALLLCGPGRALADDIGDAKNIINQAEATLKNF
                     MNDPDMKWFGSHLQEAKGVYIVPKLTKGAFIFGVEGGNGVVLAREDKSGGWSEPVFYE
                     TSAASFGLQAGAQSSEAVVLIMTPKAVDSLMANKIKLGADGSVAIGPKGGGADTNLTA
                     DFVTFTRAKGLYAGVSFDGASIRSRDELNAAYYGQDVRPSDIIIAHKAKPNSHSKSLQ
                     TMLTTASTAKP"
     gene            11315..12361
                     /locus_tag="VFH05_09175"
     CDS             11315..12361
                     /locus_tag="VFH05_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HET6678430.1"
                     /translation="MGHQWSPWWAAGICIVLIGSVSPLEKTAVGAEPPPSGLRDAIVC
                     SDLDSDVERLQCYDDEAVQRGVRRGKDSDAAAADVVRPPSLMGRRWELDPGTEHGLFS
                     IRPHKQVYLLPVRYTTNVNNQPSSPTQGTIPQSLGYSNTEVKFQFSAKLKAIEIKERF
                     DLWIAYTQLSFFQMYNKSKSAPFRESNYEPEIMGVIHTDYDLFGLKGRFINIGLVHQS
                     NGRQDPLSRSWNRIYAQFGFERGAFGLTIRPWYRIREDWTSDNNPDITAFVGRGDLVA
                     TYQKGGHNVSLLLRSNVDFWNLRGAVQLDWSFPLYRELKGYVQVFSGYGESLIDYNHN
                     QTTLGAGFLLIDWM"
     gene            12468..13088
                     /locus_tag="VFH05_09180"
     CDS             12468..13088
                     /locus_tag="VFH05_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678431.1"
                     /translation="MKPNVVVGMCLMVVLMVPSLHAQESTLPESTLKVVRIRPNLDPD
                     KLQYILSTERKEVFERNMDLDEQQSEIFWGVYHRYEKEKDQLEAKRLRLLGTYIEKYS
                     SLTNDDVMKIVKQSSENQQADLALRKKYFHIYSKKLNPVAAARFMQLDDIVGMVTRLA
                     ILGNLPLIGDVPHAAASGSSADETASPGNHDTSASSTDHPSMDLTK"
     gene            13118..13585
                     /locus_tag="VFH05_09185"
     CDS             13118..13585
                     /locus_tag="VFH05_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HET6678432.1"
                     /translation="MNCSRVIVIVIGLLFITSEALAAAEVFVYPSKGQTKEQQEQDEF
                     SCYKWAKEQTGFDPNQPMQQAAAPPPQGGAVKGAARGAAIGAIGGAIGGDAGKGAAIG
                     AGVGAAGGAMRRRHAAEQQAAAQEKAKQAYNADVGGYKRAFGACMTGRGYTVS"
     gene            13793..15064
                     /locus_tag="VFH05_09190"
     CDS             13793..15064
                     /locus_tag="VFH05_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HET6678433.1"
                     /translation="MNLDIRLPAEELAVAVDEGFPPVASREEDWNDAGTLGGQDDVRF
                     LYRLVRGSFRYRMNHDHFDVWFDHVRYRVWARKSQGQEFVEGRCGHGDDPPKRLNLAA
                     RSALTWSDQWRLQSHTTFAEPVFVEPCQLAGLDLDATPLLKSVLQPRLVALGQSIDRI
                     IRRRTEAKKRAETVWQKLQEPIELSPNQWLVSNLSDARVGPITSNGTLFVKTSVNLVM
                     EPKIVSGSKPEAVPTSLPPLQLAALPLEGFHLALPIALEYARINQRLEEEMLGQEFQT
                     PLGDTLKVEGVQFYGSGDKLILALRVSGGVNGNLYATGTPVFEEGLGILRFVDLDFTV
                     DTRNVLVRSANWMFHENILSSLKAHAVIDLSSQLQTLRSRLETALTRNLGPDARLQAE
                     VLTLRPRGVYPTASGVEVHIIADGAMWVELR"
     gene            complement(15103..15537)
                     /locus_tag="VFH05_09195"
     CDS             complement(15103..15537)
                     /locus_tag="VFH05_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6678434.1"
                     /translation="MARYRGSSSDDSVRLLLADDNPSMLQAARRILEPEFQVVGTVHD
                     GQALLEAADALRPDVLIIDISMGMLNGLEAARILTRSECKPKIVFLTVHQDQEFVEEA
                     FSVGALGYVVKPRLGTDLLPAVREALVGRAFVSPHLGSPKRC"
     gene            complement(15690..16340)
                     /locus_tag="VFH05_09200"
     CDS             complement(15690..16340)
                     /locus_tag="VFH05_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6678435.1"
                     /translation="MQRPRVLLADDHRMLAEACIRLLEPECEVVGTVTDGRALLPVAR
                     DLHPDVIVLDIGMPLLNGLEAARQIKQSMPGVKLVFMTMNEDPDLTREALHIGASGYL
                     LKSSAASELPEAIRAAVRGDTYVDGRASAKLDDSFIREGERPTAGGLTARQREVLQLL
                     VEGFSMKEAARVLRVTARTVAFHKYRVMQLFRLKSNAELIQFAIEQRVTLGTPHQH"
     gene            complement(16354..17442)
                     /locus_tag="VFH05_09205"
     CDS             complement(16354..17442)
                     /locus_tag="VFH05_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6678436.1"
                     /translation="MLTTTMSSSTPTQESFEAYSRGRSAACQPQPRYQDLVHVLDAIV
                     WRADTATLQITYVSERAKTILGYPLSQWRQPHFQETHLHPEDRLKVLACYREAIRDGE
                     NHRLDFRMTAADGREVWFHDCMHAIKVGTRKELIGVMVDITDRKAAEAGLIEMTGRLI
                     RAQEEERSRIARELHDDFNQRLALLAIGLQRLEHTIQSEIGVAAQVSDLYHMTQEIAS
                     DVHRLSHQLHPAKLQHLGLVPAIKGLCRELFEQYGAEIDFVHRNVPNPIAQESALCIF
                     RVAQEALSNTVKHSGVKNGKLELSVDRGVLHLCVSDSGAGFDLQAISAKGRLGLISMQ
                     ERVRAAGGTISVESRPSTGTRISVHLAA"
     gene            17775..>18824
                     /locus_tag="VFH05_09210"
     CDS             17775..>18824
                     /locus_tag="VFH05_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6678437.1"
                     /translation="MNQKAVAQWPRHPTIYEINTWVWLYELSERARRPITLGNVPTKQ
                     WDAIAAYGFDAVWFMGVWERSPAGVAISMRNAALVAEFRRVLPDFTDEDNVGSAYCIR
                     DYRVDARLGGPEGLAQARAQLAKRGMRLVLDFVPNHVAPDHPWARAHPDYFIQGNSDD
                     LSNNPNDFIRVGENILACGRDPYFPAWSDVLQLNAFNPHLRKAAIETVRSIAEQCDGM
                     RCDMAMLLVNSIFERTWGARAGRMPPSEYWSDVIGTVRQRFQDVLFIAEAYWDLEYQL
                     QQQGFDYCYDKRLYDRLEHGPADSIRQHLDADSAYQQKLIRFIENHDEPRAAAAFSSS
                     KAQAAAVTVSTLQGAV"
BASE COUNT         4039 a         5163 c         5479 g         4143 t
ORIGIN      
        1 aaaatcccca ggagcgccca gccgttggga catgtccttg aagaaggaga catcgaggta
       61 tagcttccga tcctgcgggc aataaaatgg cccaactgct gaggatgtcg cgccgcacgc
      121 ggaatgcacc gcgccggtaa acaacacgag gcggggattt tcgtaatggc cgcccgcttt
      181 gggaagtagc tgctgccagg tatcttcagt gtcagcgagc acgacagacg cgaattgacc
      241 gatctcgtct ttgacgggac cagtcggcac cgactcaatg ggcggctctg tcgtggtgct
      301 ctgaattccg ctcatcaggt cgataagcgt caacgggttc ataccggtaa aataactgac
      361 agccagcacc agcacgatgc cgccgaggcc gagtccgccg acgcctccga ttctcgccgg
      421 gccgaatccg cgtcggtctt ccacattctc gctttgacgt tggccctgcc agcgcatggc
      481 tctcctcagg atcagtttcg tccacgatac tgcgactcga aggagaagtc caggtgtgcg
      541 gtgggtgcga agccttcggc tgataaagcc tcgcacccat cgggtcccag ggagacgtca
      601 gttggcagga ggcaggccgc ccgcgatttg tgagagatct ttcgggtaga gcacgatctc
      661 gatgcgccgg ttggaggagc gaccctgctc tgagtcattg cttgcaatgg gatgcgtatc
      721 ggcgtatccc acggccgaga gcacttctcg ctgcacgcct ccctgatcaa tgaggtagcg
      781 caccacgatc gtcgcacggg cggttgagag ttcccaattc gtcttgaact tatcctgaag
      841 cttcgtgctg ataggtacgt tgtcggtgtg gccttcgatt ctgatctgct tatccccaac
      901 cttgctcaac acgtcgctga cttgtttcag aacttttact ccggcgggct tgatgtcggc
      961 gcggccggag tcgaacagga cacgatcgac catgttgatc gtcagacgat cacggacctg
     1021 ctgaatcgta atgtttcctt tcgcgatttc gtccttgagg gatctcgaga gttcctcctg
     1081 agtcttcgtg agacgacgga tctcgtcttc cttggctgct cgttcctgtt caagacgagc
     1141 cttgtcttcc gcttcggcct tcaggcgatt gcgctcttcg tccagactgg ccgacagttg
     1201 agattgctct tgctggagtt gctcgcgctc cttggcgagc ttcgcttttt cctctgtctc
     1261 ggtcttcaaa cgttggcgtt cctggtccag agcagctgag agcctggact gctcctggcg
     1321 caattgttcc cgctctttcg cgacccgtgc cgactcggcc tccaattcgg ccgcccgttg
     1381 ttgaagcttg atgatctcgc tttgagctgt ggccgttccg ctggcgagct gttgcttgtc
     1441 gctttcgagc gcggatacct gcgcggtctc gtcggccagt ttcttttcca gagtcgcgat
     1501 ttgtccgcgc acgtcactca actcggtccc caactggctt ttttcacgct cgaggccgtc
     1561 cagcctgttt tggagttccg ctgaacgggc ggtcaaggct tcgcgttcct tctcgagaga
     1621 agccgcttgc tgctccgcgt tctttcgctg catcgtttgc tcatccagat tgtgctggag
     1681 cccggccaat tgctgctgca gttgatccga gtcggctttg gctttctttt tcaatgcgtc
     1741 caattccgct gcctggctgg ctgacagctt cttgcttttc tccagttctt ctaatgcctt
     1801 ctgatgcgta tcggtcgaga cacagccgga tcccatgagc agggctgaca cgatcaggct
     1861 ggtctggatg aggatccgat gatggcaatc gcggcgcggt atcataagcc ctcctgtatg
     1921 aaggtgtaga tggatgatga atcaggtccg agcaaagtcg tgcccttatt tagcacgagt
     1981 cgatgaaggg ggaaagcgag aacaaacgct ggatactcaa tgacttcaat gtcttcaatc
     2041 aatgacgctt cactcaccac cgtttcgatg cgagtgggaa ggacgagcgg accctgcatg
     2101 gaccgtcgtc caggcaagcg gatcgctgac ttgtgggaca agacgattgc ggacgttcta
     2161 gaaatcggat gcaggaaggg aggcaaacgg gggcgcagcg agcgctgcgc ccctcggagc
     2221 gcgactactt gccgcccatg cccttcttat cgtccttctt gttctcgtcg tccgccttgc
     2281 taccgctcga gtccttcttc tctttctgtt ccgccggagc aggctcattc gcgaagctca
     2341 ccggcgcacc cacgcccagt gccaggaatg ctgccaatac gataaggtgt gcaaaacggg
     2401 tcatatcgaa cctccttgtg atgggaaaag aacgggtgtc aaccggtgac atccattcgg
     2461 ttaaaatgat cagcgaggcg cgatctcgcg gtgagcaatt gcgatgccgc atcacatggt
     2521 tcttcttgag ggcataaatc tagggaaatt acctgttggt gacgtcgggc aggagtcatt
     2581 tggctctcga caagtaggta caagggggaa tacgcatgaa gggacgggga taaccgtgtg
     2641 aaggggaatg catacaaacc tacatcatgg agggattgag gtcacgagcc aaaggcgaaa
     2701 cgatcccctc acaaatgtat tactgttgag ccatgtcacg aactattgtt tttcataagc
     2761 catacgatgt tctgccctgt tttaaggatc cagacgggcg gccgaccctc gccgactata
     2821 tcactgtgcc cggcgtctat gccgcagggc gcttggatcg tgacagtgaa gggctgatga
     2881 ttcttacttc tgatggtgca ctggtgcatc gcattactga tccgcagcac aaactaccca
     2941 aggtgtattg ggcccaggtt gagcgcgttc cagcggaagg agcgttgaac caattgcgcc
     3001 gcggcgtgat gctaaggggg attcgcacga gacccgccca cgtacgtctg ctcaggcagg
     3061 aaccgctgtt atgggagcgg ccggtgccga tcagggtccg ccgaagcgtg ccgacggcat
     3121 ggctcgaggt cgtgatccat gaagggaaga acagacaggt gaggcgcatg actgcagcgg
     3181 tcggccatcc gacactgcgt ttgatccgag tcgcaatcgg cgccatccgg ctcgacaacc
     3241 tgcagccagg ccaatggcgg gacgcatcca gcgctgaact gcggaccttg tgccaccagc
     3301 ctcgcttgga agcgcctgca cacgcttcta agcggcgata acctagctct gcgaaaattc
     3361 acgaaatcgt tgcattgagc gagcaccggc tcatacttac gattaacaac gtaagtagcc
     3421 accacatgct tctcaccatt cggtcgaacg gaggggacca tgggaactca ggcgcaatgc
     3481 accacttcag ggtccgtgga tcggacgata ctattgacgc tgaagcgggg tggaacatct
     3541 ttggcgggaa tcgggtgggc ccaggtgctt ctgtcggtgg accgactgag tcggagagcg
     3601 agggtctctc gaaatccggt cacaccctgt gaatatcgga tttcgatcgc ggggctcctg
     3661 aggcgctaga aggaggcgag cggacacgca taacgctcct gtgctcccgg agggcggcac
     3721 acggcactaa gcagaagcga catgcgtacc gttctgagga ggcaaacgag caagctcgga
     3781 aggatcagtt gtaccaacgg aaggaaaatc gcttaacgat ggcggatcat acggccgata
     3841 atgccgatgc cgatgcccag caggatatat cctgccagta cattgacaat gacgacccct
     3901 tggctgatga catgcgtggg ttgatagtct accaatccca gcgctgccgt cacattcacc
     3961 gaataataga cggccgacca gaacggaatc catggagcga cggcatccgt ttccatcggt
     4021 tgcagccatc gtaggtggaa gaacagctcg tacaacaggc tgaacagtcc gatcgtgccg
     4081 acgacccaca ggccccagcg cgagaagctg gtgccgtagt tactcgtgaa tttccacgcc
     4141 aactgagccc agggatgttt cccctccatc ttggcccacg tcactcgcgc gttgcgctcg
     4201 ttcagatatt cggtctgcga ccagtccaac tcgccgactt cggcgaagca atgattggcg
     4261 cgcgagtaag agcggacggc taagtgctgc agatcatgtc tcgatgaagg gcttgccgcc
     4321 gcctgagcgg cgtgttctgc caggcgtccg gcattggaat aggcgcgtcc cgcctgcgtg
     4381 aagtgtccga tccgatgcag ttgctgggcg gcctgatggt aggcctgtgc ggaatcgcct
     4441 gcattgcggc cttcaagaaa gtgtgccgcg gccagaaaat aaaaggccga ttgcatgatt
     4501 tccgtccggg cgtccttttg tccgaacgcc tcggcttttc tgaggaccgc ctctgcggac
     4561 tgggccgatt ccagctcgct cgcgccctgt cggtaaaatt cattggctag aggatcggta
     4621 aaagttcgct cagacatcga attcgatggt gcggtggccg tgggacgcca tgttactcga
     4681 aagacggccc tgcaggaaag cccatcaatc cactctctcc ttctgtaccg ctcccgtttc
     4741 accttgcggt cagtagatct ttcgattagg ctgtgcccaa tctcaaccta aggtcttggc
     4801 cctatgaaaa gactgttcta cgtgctcgtc atgacagtcg cctttgccgg atgcgcggtg
     4861 gagcaagaat attctcgtac tcccaggact gcagtggagc aactgctctt gacacaggcg
     4921 attgaacggt catttcacaa ccttcgactg gaactgcctc ccggtgcaac ccttgagctc
     4981 caggcgacca gcctcgacag cgatcgaacc ttggtacgga ttaccggccc tgaattggct
     5041 tcagcaaaac atcctgcgct cgagacgact tatattcggg atgcggtggc ggctgttctg
     5101 ggacggcatg gatatcggat accgaaccag gacacgaact atctggtccg tgtgatggtg
     5161 gagtcgctcg gaaccatgca gggattgacc ctcatcgggc ttcctcctat ccagagcacc
     5221 gtcatcccct tttcgcttcc ggaactcgcg ctatttaagt tccagaagca aagcaggtat
     5281 gccaggctgc acgtgaacat gtttgacaat cgtacggggg attcgtcggt tcgtccgcga
     5341 cgatcatcgg acggacgtat tatgatcagt atacggtgct gttttatttc acctgggccg
     5401 caacggattt gctcgctcca ccatgaggct caagagcgta aaggcagacc gctttggctg
     5461 actgctatga gtgtcgcttg aagtattgca cctcccgttc atgcttctga gccgccgccc
     5521 cggtcttgaa cgtacccagg ttgcgtcgct tgcctgtttt gggatccttc ttgcgagaat
     5581 agaggcggta gccgccagac gataattttc gtatcatgac ggtggtcctg tcgtaccccg
     5641 atcccgattc atgactgaag caggcgtagg agcagcggga cgaccttatc agacttccga
     5701 cacctcggag atgatcgtgt gatagcccga cgctcccgcg ggttgcggtc tgaccagctc
     5761 ggcaatctgc aattgtccct tcgtgtcgat tgcccggacg acggtctggt attttccggg
     5821 cttcggcggc tgccaggtgt agctccacag gacccatgaa tagggagaca tgggcggctc
     5881 gatcatacaa tcgttccacg tgcggccggc atcgaagctc aattcgacct tgccgatact
     5941 gttgggccca ccgaaggcaa tgcctcggaa cgtctgctgg tctcctcgca atgtttgata
     6001 gtgccctggc gagtcgattc gcgaaaagat cttaatggtg ccgtcgtctg tccatccttt
     6061 gcgttgccag tacccctgat agtcccccgg atacacttcg atttcgtaaa tccatttcac
     6121 gttcttgatg ccgtataagc ccggcacgat ggcgcggagt ggaaacccgt gttccttggg
     6181 cagtttctcc ccattcatga gaaaagcgag catcacgtca tcctgcatcg cccgtggaaa
     6241 gggaatgctg tcgtcgtatc catcgatgcc gcggaacacg acgtcccgtg ccgtgtcttc
     6301 gtcagccccg gcgtcctgaa ggagtttctt caatgaaatg ccccgccaag tggcattgcc
     6361 aaggctgtcg ccgcccggca aggtgtcgat gcacatcaat gtcgacacct gatcgtacga
     6421 atcacgattc agaatgtccc tccagccaag tgacatgggg gttttgacgt cgcctttgac
     6481 ggtcagtttc cattggtcga tattgatctc gcgcgacacg gacacggcgc tgtcggcata
     6541 atttacgaca tagaatttgg agttgggtgt gaaataggtc gtctcgcgag gaggaaccgc
     6601 gaacatgcga ccgaagacac cgccgacggc gtcacagccg ccgaccatgc cggccaacgc
     6661 ccccagtccc gtcgcctgaa gcaatgtccg tcgcgaaagc cgcatgtgct cattatcgta
     6721 catggaacca agggaccgca atgagcgacc atcggctcca cgctgtctca tagaggctgc
     6781 gacggatgga ggactacctc gcggttttgc gcggtttaca atgagcccag cgtactgcgc
     6841 aaggggaact cgtgagttga ccgcgactcc aatgtcgagt gctcttcgag agctcgagac
     6901 tgccaagcta ccgtccgaca aggtatggaa caagaaacac atgtcatgat atgaatcgct
     6961 ggtccccaga gcgcgacaac ggcgtcaggc tgcaggaggt tctgcatgaa tcaccctttc
     7021 cggttgagaa aatcgtggat cgcctgccca ctcattttca tggtctgtgc actggtcgcc
     7081 tgcgccggag gaccgtctca atctgatccc tcgtggaagt ccgtgccgat tactgatgtg
     7141 tcgatggtgg taggggagtg ggacggaacc gtgacaaagg acaaggccat gtttccggag
     7201 ggtgcggttc gcctgatcat ccgtgccaac agcacctatc tgttcgcggg tgaaaccttg
     7261 tccaaatcgg ccgtcggttc cggaggagtc gaaacgcgcg agggtcgatt gatcggtgac
     7321 acggagcatc gcgctgtgac atttaccctt tatgaccaca aaggggcgcg gatgctcgtc
     7381 gtagatacga ccaacaaaga aaacggcacg caatatcatg gacaatttac gaaggttcgg
     7441 taatgatcag actcatgtga agtcgccggt actctctatc ctcacaggac cccacccgca
     7501 catccaatag ggactcacgc tgctcctgtt atttttgaca gtttccccgt ttgcccagac
     7561 atgatacccc acacgtctcg atagtcgccg tatccggccg ctatctggtg ggacaggggc
     7621 ggctgcggcg tgacctgcat cggcgatgtt tcaggtgcat ctggtgcatc cctatgattt
     7681 catgcagcaa tcacgaaccg agtgagcgga gcagtcagac ctacttcact gctctccaga
     7741 gtgggctgat cctggtgttg gccctgaata gctgcggcga agatcgagtg acagtcttgg
     7801 tggaggcggc ggaagagagg atgaacccgc tttccgcggc cgacgaccat gccagcgagg
     7861 ttcgcctcca tcaagccctg gtcgatcatt tcggattctc agccctcggt ctttcgacct
     7921 atgttttcat gaacagggga tatgtcgtcg gatatgtcga cagccttgag caggctcagg
     7981 cggtcttcga aaccgcgaaa aatgtcaaag gccttcaatc ggttgaggcc gtgcttcctg
     8041 tgaaacagcc gccgcctaca gatgtcggaa aagtgatttc agattcaact ctgaagtctc
     8101 agatcgagtc cgcgctcacc cgcggtaccg ctatggtgtc gggccgggtg catgtggagg
     8161 tgctgaatgg ccgggtggtg ctcttgggcg tcgtctctgg cagcgatgaa cgggaacggg
     8221 cagaacgagc ggcggcggga acgatcggag tcacacgagt caccaattgg ctgttgctgc
     8281 cggaaacaga gtatatggcg atccggtcgc aagtcttcta ggagtggctc atgaagccgt
     8341 cttgtcgtct ctgcctcctt gtcttggttc tcgcgatttc gcctgtctct tgtgctgcag
     8401 cacggcatgg gacaaccgtg ccgaagccat ccggtttgcc catgcaacgg gtcaccgtgc
     8461 tgtcctacaa cacgctgcat gggctggagc ccagcggcct gatcgttaaa ccgagcgaat
     8521 cgaatgaggc acgccaggcg aggcttgatt tacaattcga acaactcgcg gtggcccgac
     8581 ctgagctcat cctcctgcag gaggtcaatc ctctgcctga aatggccgag acgtatgtcg
     8641 ccgcgatgaa acgcgctggg ttggagtata gccaagtgca tcaagtcgat gcctgcgggg
     8701 tacgccttgc cccagggctc gctgtggttc cccatctgaa caatgggttg gtggtccttg
     8761 ccaaagcgcc tttgagatta agaaaagttg cggggctcaa gctaagcgga ggggtcggac
     8821 gctgcgtcga ttacatgggt ctgcagaccg gcgaacttcg ctatgccttg gtcgccgaag
     8881 tcgaaaaccc ggatacagga aagaagctgc tggcggtgag tcttcatcta cattccggta
     8941 ttgaacgcaa cgcgtttttt attcaaaaga tcaatgaggc tgttgagcaa ggaagggcac
     9001 ggcgggagga cttcgagggt atcgtcgccg cgatcgaaca tgaccagaac aggcgccttg
     9061 atgagattcg cctcctcgtc caagagatac agaaacttga tgcaaggggc caatacatcg
     9121 gaacactcgt cggcggagat ttcaattttg agccggatga tcctgaatat cgagagcttg
     9181 aacgggcggg gctaacagat acctacgtga tgggcaactc ccagagcgaa atctacagtc
     9241 tggaccctca gaagaacgtg attgcggcaa agggagtgcg agaggtgccc tctgcgcttc
     9301 gcgaggcgat gaagcggttg cctcagagcc agcaacagaa aattcttgag ggctatcaaa
     9361 cgggtatcag ccaggcgagg cgagttgatt ttctcttcct catgaggaaa ccctcggacg
     9421 gtcccacagg gtgctttcat caggaattat tcggcaaacc ggcaatggtt tcaactcaac
     9481 cgggatcgga tcactatggt gtgctcgtga cttatctcgc tgacgcgtcc cgatgttgac
     9541 caggcagcct catgcttgcg atctgattag gtgaatggtg cgccatgttg aacatgtcaa
     9601 tgcccttgca tgaacttatc ggcttcgtgg cgggcttcgg caccacattt gccgccatgc
     9661 ccgatctcat ctccatgctc aagcggcgtt cctgtgtagg catgaatccg acgatggccg
     9721 cgattatggg cacctttcaa atcgtgtggg tctattacgg cgtcttggtc gaatcgaggc
     9781 cggtcattgt gtggaatacg atcgcagtgg ggatcaattt cctggtagtc ggtgcctatg
     9841 tctattttgc ctgcagccgg ccgcgccatg cgcaggttgt caacggttca cattcgagcg
     9901 aaggtcccag gaactctccg ccacgatagg ttgtcctgaa gaataggcga gctacggatg
     9961 gccggcactg ttggaagtgg aggaatctca tgcgtcctgg aagaagggcc tgcctgctca
    10021 tcctggcgat gagcctaacg gcctgtagct cttttaaggt gaacactgat cacgatccga
    10081 ctgtagattt ctcttcgttc aagaccttcg cctttgccgg accgtccgag gcgaataaag
    10141 gtggaatcta cgataattcc ttgacgcgaa aacgtattga gtctgccgtc gtgcgtgagc
    10201 tcacgggaaa agggctccgc caagtaggac tcgatgaacc gcaggacctt ctcgttcact
    10261 actgggtcgg catccaagac aaacaacgac tcgagagcac ggggccgacg gtcggcgttg
    10321 cccgtggacc gggaggtggt tacggatggg gcgccggcta tggcggcggc gtgacgacct
    10381 atgaatataa ggaaggaaca ttggtgttag atctgattga accgccgaag aagcagctgg
    10441 tctggcgggc cacgatcgtg ggcacattgc aggactcccc aacagacaat gtcgaccttg
    10501 cgaacaaggc catcgccaag gccttcgaaa ggtatccgcc gcctgtgaat accccgtgaa
    10561 cttttaaaca aggagggaca accatgacaa agtcagcgac cacgaccgcc gcactaatcg
    10621 ctctgctgct ctgcgggcca ggccgagctt tggcagatga tatcggcgat gccaagaaca
    10681 tcattaatca ggccgaggcc acgcttaaaa acttcatgaa cgatcctgac atgaagtggt
    10741 tcggcagcca tctacaagag gccaagggcg tctacattgt gcccaaactt acgaaggggg
    10801 ccttcatctt cggcgttgaa ggcggcaatg gcgtcgtact cgcgcgagag gataaatccg
    10861 gtgggtggag cgaaccggtg ttttatgaaa cgtcggcggc cagttttggc ctacaggcag
    10921 gagcccagtc atcagaagcg gttgtgttga ttatgacgcc taaagcagtg gattcgctga
    10981 tggcgaacaa gatcaagctt ggggcagatg gatctgttgc catcgggccg aagggaggag
    11041 gagccgacac gaatctgacc gctgatttcg tgaccttcac acgtgcgaag gggttatatg
    11101 ccggggtctc attcgatgga gcatcgatcc ggtcaaggga cgaactcaat gccgcctatt
    11161 atggacagga cgtgcgtccg tcggacatca ttattgccca taaggcgaaa ccgaactccc
    11221 actccaagtc attgcagacc atgctgacga cggcttccac ggcgaaacca tagaagacgc
    11281 gaagggtgga agaagcctga ccaccagcag agccgtggga catcagtggt caccttggtg
    11341 ggcggcaggc atctgcatcg tgctgatcgg cagcgtgtct cctctcgaga agacggccgt
    11401 tggtgcggag ccgccaccct ccgggctccg tgacgcaatc gtctgctccg atctggactc
    11461 ggatgtcgag cggttacaat gctatgacga tgaggcggtg cagcggggcg ttcgacgtgg
    11521 caaggattcc gacgctgcgg ccgctgatgt ggtgcggcca ccctcattga tgggcaggcg
    11581 ctgggaattg gatcctggca cagagcatgg gctcttttcg atccgtcccc acaagcaagt
    11641 gtatctgctg ccggttcgct ataccaccaa tgtgaacaac caaccgtcga gtcctacgca
    11701 aggtacgatt cctcagtcgt tggggtatag caacacggag gtcaagttcc aattctctgc
    11761 gaaactcaag gcaatcgaga tcaaagaacg attcgatttg tggatcgcct acacgcaact
    11821 ctcgtttttc cagatgtaca acaaatcgaa atcggcgccc tttcgcgaaa gcaattatga
    11881 gccggaaatc atgggcgtga ttcacactga ctacgatctg ttcggactga agggacgatt
    11941 catcaatata ggccttgtcc atcagtccaa cgggcgtcag gacccactgt cgcgaagctg
    12001 gaatcggatc tacgcacaat tcggattcga gcgaggagcg ttcggactga ccatcaggcc
    12061 gtggtaccgc attcgcgaag actggactag tgacaacaac cccgatatca ccgccttcgt
    12121 cggacgcgga gatctcgtcg cgacctatca gaagggcgga cacaacgtct cactccttct
    12181 gcgcagtaat gtggactttt ggaatttgcg cggcgcggtt cagttggatt ggagttttcc
    12241 attgtaccgg gaactcaagg gctatgtgca ggtgttctcc ggttacggcg aaagcttgat
    12301 tgattacaat cacaatcaga cgacgctggg agccggattt ttgctgatcg actggatgtg
    12361 agatgcttca gctgtcattt ttgacagtgt ctgtgcctgt caaatgatga tagcgtgaat
    12421 gtcggcgaaa acgtgcatct acctgctgtg aggaggaagg agccgtcatg aaaccgaacg
    12481 tggtggtcgg catgtgtctc atggttgtgt tgatggttcc gagtctgcat gcacaggagt
    12541 caacattgcc cgaatcaaca ctgaaggtgg tgcgcattcg gccgaatttg gaccccgaca
    12601 aactgcagta tattttgtcg acggagcgca aagaggtgtt cgaacggaac atggacctgg
    12661 atgagcagca aagtgagatc ttttggggcg tgtatcaccg gtatgaaaag gaaaaggatc
    12721 aacttgaggc gaaacgactt cgcttacttg gaacctatat tgaaaaatat tcatcgctga
    12781 cgaacgatga tgtgatgaag atcgtcaagc agtcgagcga gaatcagcag gccgaccttg
    12841 cactgagaaa gaagtatttc cacatttaca gtaagaaact gaatccggtg gccgccgctc
    12901 gcttcatgca gttggacgat atcgtcggga tggtcacacg tctcgcgatt ctgggcaatc
    12961 tgccattaat cggagatgtt cctcacgcgg ccgcttcggg atcctccgca gacgagaccg
    13021 cgtctccagg caatcatgac acgtccgctt cgagcacgga ccatccctcc atggacctga
    13081 cgaaatgact ctgtgtaaga ttcgtgagga ggcatgtatg aattgttcac gagtgattgt
    13141 catcgtgata gggctgctgt tcatcacgag tgaagccctt gcagctgcag aggtgttcgt
    13201 gtatcccagt aagggccaaa cgaaggagca acaggaacag gacgagttca gttgctacaa
    13261 atgggccaaa gagcaaaccg gattcgatcc gaaccagccg atgcaacaag ccgctgctcc
    13321 gccgccgcag ggcggcgccg tgaagggcgc ggcgagaggc gccgcaatcg gcgcaatcgg
    13381 gggtgccatc ggcggcgatg caggaaaggg agcggccatt ggagcgggag ttggcgctgc
    13441 aggaggtgcg atgcgccgcc gtcacgcagc ggagcagcag gctgcggccc aggaaaaagc
    13501 aaagcaggcg tataacgcgg acgttggcgg ctacaagcgc gcgtttggcg cctgcatgac
    13561 gggacggggc tacaccgtca gttaaccggt gtctgatttc ttcaccgcag ggagcgaatt
    13621 gacgcgtaca ggcagtgact caagacaaag atggttcggc acattgtgct gttcactctt
    13681 actgccgtcc ttctatgcga tcagcgcatg ggcagactcg ccactgaaca ttccaaagcc
    13741 aaacgatccg ctcaagcctg cgcctccgcc gcctgccttg gaggcgtctg tcgtgaatct
    13801 tgatattcga ctgcctgcgg aagaacttgc tgtcgccgtg gatgagggct tccctcctgt
    13861 cgcctcgcgg gaggaggact ggaacgatgc gggaaccctt ggagggcagg atgatgtacg
    13921 gttcttgtat cgcctggtca gagggtcgtt ccgataccgg atgaaccacg atcattttga
    13981 tgtgtggttt gatcacgtgc gctatcgggt ctgggcccga aagagccagg gacaagagtt
    14041 tgttgagggc cgttgcggcc atggcgacga tccaccgaaa aggctgaatc ttgccgcccg
    14101 ctctgccctg acctggtccg atcaatggcg ccttcaatct cacacaacgt ttgcggagcc
    14161 ggtgttcgtc gaaccctgcc agctggcggg ccttgatctc gatgccacgc ctctcctgaa
    14221 atcggtcctg caaccgcgcc ttgtcgcgct aggtcaaagc atcgatcgga tcatacgccg
    14281 acgtacggaa gcgaagaagc gcgccgagac ggtctggcag aaattgcagg aacccatcga
    14341 attatctccc aaccaatggc tcgtctccaa tctcagcgat gcgcgagtcg gcccgatcac
    14401 gtccaacggc acactgtttg tgaagacgtc cgttaatctg gtaatggagc caaaaattgt
    14461 cagtggctcc aagcccgagg ctgttcccac gtcgctgccg ccgctccaat tggctgccct
    14521 gccgctcgaa ggctttcacc tcgcactgcc gatcgcgctc gaatacgcac ggatcaatca
    14581 acggcttgaa gaggagatgc ttgggcagga gtttcagaca cctctcggcg atacgttgaa
    14641 ggtcgagggc gtgcaattct acggaagcgg cgacaagctg atcctcgcgc tccgggtgtc
    14701 tggaggagtg aacggaaatc tctacgcgac tggcacaccg gtgttcgagg aaggcctcgg
    14761 gatcctccga ttcgttgatt tggatttcac ggtggatacc cgcaatgtgc tagtccgttc
    14821 ggcaaactgg atgtttcacg agaacatctt gtcgagcctg aaggcccatg cggtgatcga
    14881 tctctcgagc cagcttcaga cgcttcgatc acgtctcgaa acggctttga ctcgaaatct
    14941 ggggcccgat gcccggcttc aagcagaagt tctgacgctt cgtcctcgcg gcgtgtaccc
    15001 cacagccagc ggagttgaag tgcatatcat cgcggatggc gcgatgtggg tcgaattacg
    15061 gtgagggagc tgggagcaac cgccgttcgc agacgcatgc catcaacacc tcttcggtga
    15121 tcccaggtga ggtgagacga aggctcttcc caccagcgct tcccgaacag caggcagcaa
    15181 gtccgtgccg agccgcggtt tgacgacata gcccagcgcc ccgacggaaa aagcttcctc
    15241 gacaaattcc tgatcctggt gaaccgtaag aaagacgatc ttgggcttgc actcactgcg
    15301 tgtcagtatg cgtgcggctt ccagaccatt cagcatcccc atggatatat cgattattaa
    15361 gacatcaggc ctcagcgcgt ctgcggcctc aagaagggcc tgaccgtcat gaacggtgcc
    15421 aaccacctgg aactcaggct ccaggatacg gcgcgcggcc tgcagcatcg atgggttgtc
    15481 atcggctaag aggagacgta cggagtcgtc agaactgctt ccccgatatc gagccatgga
    15541 tgctccatgg aaaaggagat gcgagcgcag cttcttatca cttccccctt gggggcgtaa
    15601 ctggcaaaag taacagattc acttggtcct ctccggatgg agaccggcaa gagagatcgt
    15661 aatataggcg tgcaggcagc gtggcgtggc tagtgttgat gcggagtgcc caaggtaaca
    15721 cgctgctcaa tcgcgaactg aatgagctct gcgttacttt tgaggcggaa tagctgcatc
    15781 actcgatatt tatggaatgc gaccgtacgc gcggtcacgc ggagaacacg ggccgcttct
    15841 ttcatggaaa acccttccac aagcagttga agcacctcgc gctggcgcgc agtcagtccg
    15901 ccggctgtgg gacgctcgcc ttctcggata aaggaatcat ccaacttggc agaagcacga
    15961 ccgtcgacat aggtgtcgcc acggactgcc gcccggatgg cttcgggcaa ctccgaggcc
    16021 gctgaacttt tgagaaggta tccagacgcc ccgatgtgaa gggcttccct ggtgagatcg
    16081 ggatcttcat tcatcgtcat gaataccaat ttgacgccgg gcattgattg tttgatttgt
    16141 cgggccgctt cgagtccgtt caacaacggc atgccgatgt ccagtacgat gacatcagga
    16201 tgaagatcac gagcgacggg cagcagcgcc cgtccatccg tcacggtgcc aacgacttcg
    16261 cattccggct ccaaaagcct gatgcaggct tcggcaagca tgcgatggtc atcggcgagc
    16321 aagacgcgcg gacgctgcat ggaatcctcc catctaggcg gccaaatgca ctgaaatgcg
    16381 cgttcctgta gacggacggg attcgaccga gatggtaccc cccgcggccc tcacccgctc
    16441 ttgcatgctg atcaagccaa gccgtccttt ggctgaaatg gcttggaggt caaagcccgc
    16501 gccggaatct gagacgcata aatgcaagac acctcgatcc acggacaact cgagctttcc
    16561 attcttcacg ccgctgtgct tgaccgtatt gctcaaggct tcttgcgcga cacgaaagat
    16621 gcaaagcgcc gattcttggg cgatcggatt cgggacattg cgatggacaa agtcgatctc
    16681 agccccatat tgctcgaaca gctcacgaca taatcccttg atcgccggga cgaggcctag
    16741 atgttgcagt tttgcggggt ggagctggtg ggacaggcga tgcacgtccg atgcgatttc
    16801 ttgtgtcatg tggtagagat ccgagacctg agcggcaact ccgatctccg attgaatcgt
    16861 gtgctcgaga cgttgtaaac caatagcgag cagcgccaac cgttggttaa aatcatcatg
    16921 cagttctcga gcaattctgc tgcgctcctc ctcctgcgct cggatcaagc gcccggtcat
    16981 ttcaatcaaa ccggcctcgg cggcttttcg gtcggtaatg tccaccatga cgccgatcaa
    17041 ttccttgcgt gtcccgacct tgatggcatg catgcaatcg tgaaaccaga cctctcgacc
    17101 atcggctgcc gtcatgcgga aatccaatcg atggttttca ccgtctcgaa tggcctcacg
    17161 gtagcaagcc agtaccttta atcggtcctc gggatgcagg tgggtctcct ggaagtgtgg
    17221 ttgtcgccac tggctaagcg ggtagccgag aatggtcttg gcccgttcac taacatacgt
    17281 gatctgaagc gtcgcagtgt ctgcgcgcca cacaatggca tctaacacat gcacgaggtc
    17341 ttggtagcgc ggttgaggct ggcatgccgc acttcttccc cgagaatatg cctcgaagct
    17401 ttcctgggtt ggcgttgaag atgacatcgt cgtggtcagc atgctgacct cccatagagt
    17461 gagtatgctg tgagggatgc ctagaatgaa cctgtctcgt attcgcagtc aagagcgcct
    17521 accggctttt gtcctataga gcagcgatgt tacaaccagg ttaccgtaag agccggctaa
    17581 acaaagattc atgcctagag gattcaacgt gcttccttca ctgtcaaatc tgacagtgtt
    17641 cttgagcact tccgcattgt aaagtgcttg ctctgacttt cgctgccaat ccatgaacga
    17701 agggactcgc tcgagaccgg aatggtcttc ggcgtgagaa ggagtcggcg gccttgaacc
    17761 ctgccgacgt ctgaatgaac cagaaagccg tggctcaatg gccccgtcac ccaacgattt
    17821 acgagatcaa tacgtgggtg tggctctatg aactgagtga gcgtgctcgg cgccccatca
    17881 cattgggcaa cgtgcccacg aagcaatggg atgccattgc ggcctatgga tttgatgccg
    17941 tctggtttat gggcgtatgg gagcgaagtc cggcgggtgt cgcgatctcc atgcgaaatg
    18001 cagcgcttgt cgcggagttc cgccgagtgc tgcccgattt taccgacgag gacaatgtcg
    18061 ggtccgctta ctgtatccgg gattatcgcg tggacgctcg actaggtgga cctgaagggt
    18121 tggcgcaagc cagagcgcag ctggccaaac ggggcatgcg attggtcctt gattttgtcc
    18181 ccaaccacgt ggcgcccgac catccctggg ccagggcgca tccggactac ttcattcaag
    18241 ggaactccga tgacctgtcc aacaacccta acgattttat ccgggtcggg gagaacattc
    18301 tggcctgcgg ccgcgatccc tattttccgg cctggtcgga cgtcttacaa ctgaatgcgt
    18361 tcaatcctca cttgcgaaaa gcggcgatcg aaacggtccg gtcgattgcc gagcagtgcg
    18421 acgggatgcg ttgtgatatg gccatgctac tcgtgaattc catttttgaa cggacctggg
    18481 gagcacgggc cggtcggatg cctccaagcg agtattggtc ggacgtgatt gggacggtca
    18541 gacagcgctt tcaggacgtc ttgttcatcg ccgaagcgta ctgggacctt gagtaccaat
    18601 tgcagcaaca agggttcgat tattgttatg acaagcggtt atacgatcgc ctggagcacg
    18661 gtccagctga cagtatccgt cagcatctcg acgctgactc tgcgtatcag cagaagctga
    18721 tccgcttcat cgagaatcat gatgagccgc gtgctgcagc ggcgttctcc tcctccaagg
    18781 cgcaggcggc ggccgttacc gtatcgaccc tgcagggcgc agtc
//
LOCUS       DASSCQ010000133         6530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1747554, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000133 DASSCQ010000000
VERSION     DASSCQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747554"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFH05_09215"
     CDS             complement(<1..749)
                     /locus_tag="VFH05_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET6678438.1"
                     /translation="MKQGARWGPMGWSAMAVIVMIGWTVYLSPVGAAPPAKELDPVSM
                     VTIPAGEFLMGNPEGVGRADEWPQRPVYLDAFRIDQVEVTNERYMAFVRATGHSNPPN
                     PYGTGPLVSINGIEQLPVVQTTWYDAKAYCSWAKKRLPTEAEWEKAARGTDGRKFPWG
                     NEAATPKRANFDREWDDERTLSPVGSLPGGDSPYGVKDMAGNAREWVSDWYDADYYRH
                     APDRNPAGPDKKGVVRSIRGGSWHSPVSDITT"
     gene            complement(746..1114)
                     /locus_tag="VFH05_09220"
     CDS             complement(746..1114)
                     /locus_tag="VFH05_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678439.1"
                     /translation="MTQHRRFLLVGLFCALLGTSSLQASPIDPGRHPHPVHAQAVHEA
                     EHSVDHAWEVYHRAALGGTIASPALQVEIEQHLHEARTLVTQAQEAAERGDKRQVERL
                     IGQIEIHTSHAIEGSKEHKK"
     gene            complement(1167..2219)
                     /locus_tag="VFH05_09225"
     CDS             complement(1167..2219)
                     /locus_tag="VFH05_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET6678440.1"
                     /translation="MAVTQMVTGFVAGIVMLAGSGHAEEPLIPRGMVYIAHGPSVMGI
                     DKDESPDSAKRLTAYEKRMKTPWSAEALNDEVPAHTVFLDSYLMDKYEVSNKEYGEFV
                     KAKGHPAPAYWDDPRLNKPEHPVVGVNWEDAKAFCEYRGKRLPTEAEWEKAARGPKAN
                     LYPWGNEFDSAKVNYGKNHDATMPVDAYPEGVSYYGLYNMAGNVFEWVSDWYDPRYYG
                     RLETMVNPTGPAKPLWLGGTGTYVDRLTVGEKRVIRGGSWIAPEGTVRATHRFWNHPL
                     NNSYGVGLGFRCAQTAPPEIEQRIRDASVSVLVEMGRERFAEAEQAALLGLAIDPKNV
                     ELLELKSLIESSMKRP"
     gene            complement(2294..3160)
                     /locus_tag="VFH05_09230"
     CDS             complement(2294..3160)
                     /locus_tag="VFH05_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET6678441.1"
                     /translation="MVLIPRGEFTMGSHEHADEATHQVVLDPYYIDKYEVSNARYKEF
                     ISAAGHPAPAYWDDPRLSKPNQPVVGVSWNDATAFCKWESKRLPTEAEWERAAKGPEG
                     DNHYPWGHKLDSTKANYGQNVGRTMPVDSYPDGVNGFGVYNMAGNVFEWVSDWYDPKY
                     YKQSVALNPQGPEKGYNFANQGPVKVLRGGSWLAPETSLHTSHRFWNQPENNSYGVGL
                     GFRCAKSAPAISDEAMQEGRDAFVQALVAMGAEKNTDAMAAIEKALAADPRNKEYLAT
                     RDLIKKSMKKKN"
     gene            complement(3336..3683)
                     /locus_tag="VFH05_09235"
     CDS             complement(3336..3683)
                     /locus_tag="VFH05_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678442.1"
                     /translation="MTTRGAVHFSLLMLLVFAAGCAANFSQPPSEQSTAQTFVTQDQA
                     RQMAYSYRRQAQDLRDLARRLETEALLSSGQSSTDSKQANMLEVKNVLAAADDADELA
                     REYRRQVPHGQLQ"
     gene            4071..4994
                     /locus_tag="VFH05_09240"
     CDS             4071..4994
                     /locus_tag="VFH05_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET6678443.1"
                     /translation="MNTHRIHRVAKLVGLSKDVIRVWERRYGLVKPSRSNNRYREYTD
                     EDIALLRFLKEEVARGQTIGGLAVEGRDAVLQRMRTTSAPTPQELEPHEHLLDELASL
                     LEPLEKTRFEQKLNGAVAVIPFEEAVQRILLPLQRRVGDLWHEGKLNVAVEHYVTKLI
                     QQKLFSVMNQLSINESGPRIVVACPEGESHELGAQAVAYLAAVRGCHVYYLGPNLPAV
                     ELAAFCERVRPDLVLLSLTEITSDQTARRLLDELGSLAVKWPVAMGGQGARTIDHHLL
                     NKYKIGLLDDLTALHNRIMTLISNRMLASRP"
BASE COUNT         1411 a         1882 c         1690 g         1547 t
ORIGIN      
        1 gttgtgatgt ccgagaccgg actgtgccag gacccgcctc gaatcgagcg gaccacgcct
       61 ttcttgtccg gtcctgccgg atttcgatcg ggcgcgtgcc ggtaatagtc ggcgtcatac
      121 caatcggaaa cccattccct ggcattgccg gccatgtcct tcacgccata cggtgaatct
      181 ccacccggta acgaccccac tggacttaga gtcctctcat catcccactc tcggtcgaag
      241 ttcgcccgtt tcggcgtcgc cgcttcgttg ccccagggaa attttcggcc gtccgtgccg
      301 cgagctgcct tttcccattc cgcttcagtc ggcagccgct ttttggccca actgcaatac
      361 gccttggcgt cgtaccacgt cgtctgaacc accgggagtt gctcaattcc gttgatggac
      421 actaatgggc ccgtgccata ggggtttgga ggattgctgt gtcccgtcgc cctgacgaac
      481 gccatgtacc gctcattggt cacttcgact tgatcgatcc tgaacgcgtc caggtacact
      541 ggtctctgcg gccattcatc agctcggcca actccctccg gattgcccat caggaattct
      601 cccgcaggaa tggtgaccat cgacaccgga tccagttcct tggccggagg cgccgctcca
      661 acaggcgaca aatagacggt ccaaccgatc atgacgatga ccgccatcgc gctccacccc
      721 attggtcccc accttgcacc ttgtttcatt tcttgtgctc cttgctccct tcgatggcgt
      781 gggaagtatg gatttctatc tgaccgataa gacgctccac ctgacgctta tccccgcgct
      841 cggcggcctc ttgcgcctgg gtgacgaggg ttctggcctc gtgcaggtgc tgctcgattt
      901 caacttgcag cgccggcgag gcaatcgttc cacctaacgc cgcacgatga tagacttccc
      961 aggcatggtc aaccgagtgt tcggcttcat gaaccgcctg cgcatgcacc gggtgcggat
     1021 gacggcccgg atcgataggg ctcgcctgaa gggacgaggt cccgagcagt gcgcagaaca
     1081 aacccacgag taagaaacgt ctgtgttgtg tcatcggtac gcctccggaa aaatgacgcg
     1141 cttcactgtg acgcatgctc gttctctcac ggccgcttca tcgatgactc gatcaacgac
     1201 ttgagttcga ggagctcgac attttttgga tcgattgcca atcctaagag ggctgcctgc
     1261 tcggcctctg cgaaccgttc acgacccatt tccactaata ccgacacgga ggcatccctg
     1321 atccgctgtt cgatctcggg tggagcagtt tgggcacagc gaaatcccaa acccacgcca
     1381 tagctgttat tgagcgggtg attccagaag cggtgtgtcg ccctgaccgt gccttccggc
     1441 gcgatccacg acccgccgcg aatgacccgt ttttcgccga ccgttaaacg gtccacatag
     1501 gtgcccgttc cccccagcca caagggcttg gccggaccgg tcggattcac catcgtttca
     1561 agccggccat agtaacgagg gtcgtaccag tccgagaccc actcgaaaac attcccggcc
     1621 atgttgtaca agccgtagta gctcactccc tcgggatagg cgtcgacggg catcgtcgcg
     1681 tcgtgatttt tgccgtaatt taccttggcc gaatcaaact cgtttcccca gggatagaga
     1741 ttagccttgg ggccacgggc ggccttttcc cattccgctt ccgtaggaag gcgcttcccg
     1801 cgatattcac agaaggcttt ggcgtcctcc caattcactc cgaccactgg atgctcaggc
     1861 ttattcaagc gaggatcgtc ccaataggcc ggggccggat ggcccttggc cttgacgaac
     1921 tcgccgtatt ccttgttcga cacttcgtac ttatccatga gataagaatc tagaaacacc
     1981 gtatgggccg gaacttcgtc gtttagggct tcagccgacc agggcgtctt cattcgcttt
     2041 tcgtaggccg tgagcctttt agctgagtcg ggagattcat ccttatcaat ccccatcacc
     2101 gatggaccat gcgcgatgta taccatccct ctggggatca acggttcctc cgcatggccc
     2161 gaaccggcca acattactat gcccgcaacg aaccccgtca ccatttgggt aactgccatc
     2221 gacatcgtag acctcctcac acactctgct tctgcagggg cgtcatcgcc gcccccttga
     2281 gacgacgggt ggactaattt ttctttttca tgctcttctt gatcaaatcg cgggtcgcga
     2341 gatactcctt gttgcgagga tcggcggcca gcgccttttc aatagcggcc atggcatcgg
     2401 tgttcttctc agcacccatg gcgactaatg cctgaacaaa ggcatcgcgt ccttcttgca
     2461 tggcttcgtc cgagatcgcg ggagccgact tggcgcagcg aaatcccagt ccgacgccgt
     2521 acgagttgtt ttccggctgg ttccagaatc gatgactggt gtgtaatgat gtttctggcg
     2581 ccagccagga cccaccacgc aagactttaa ccggcccttg gttggcaaag ttgtagccct
     2641 tctccggccc ttgaggattg agcgccacac tctgcttgta gtacttcgga tcgtaccaat
     2701 cagagaccca ctcgaaaacg ttaccggcca tgttgtacac accgaagccg tttaccccgt
     2761 cgggatagga atccaccggc atcgttcgcc caacattctg cccataatta gccttcgtgg
     2821 aatcaagctt gtggccccag gggtaatgat tgtcgccttc cggtcccttg gcagcccgct
     2881 cccattcggc ttccgtcggt aaccgcttgc tttcccattt gcagaaggca gtcgcatcgt
     2941 tccagctcac gcccaccacc ggttgattgg gcttgctgag ccgtggatcg tcccaatacg
     3001 cgggggcggg atgtcccgca gcgctgatga actccttgta gcgggcgttg gagacttcgt
     3061 atttgtcgat atagtaagga tcgagcacca cttgatgagt cgcttcgtca gcatgctcat
     3121 gactccccat cgtgaattcc cctcgaggga tcagcaccat atccttctcc gtcattgtcg
     3181 tatctgcgta cgacgcagct gtaggtccgg tgattagcaa taccccgaac cctagtgtga
     3241 gaaatgtgag actccgcatg ggaaccctcc ttggttgata cgccagaacc cctggctggt
     3301 tgcaacacgt cgattacgca atcaggtgaa ccggttcact gcaattgtcc gtgcggtacc
     3361 tgacgccggt attcacgcgc cagttcgtcc gcgtcgtccg ctgccgccag aacgtttttc
     3421 acttcgagca tgttcgcttg cttactgtct gtgctggatt gaccgctgga taaaagggct
     3481 tcggtctcca accggcgggc caggtcgcga agatcctgag cctgtcgacg ataggaatac
     3541 gccatctgcc gtgcctggtc ctgcgtcaca aaggtctgag ccgtagactg ctcacttggt
     3601 ggttgtgaaa agttagctgc gcaaccagca gcaaagacca acaacatcag taaggaaaaa
     3661 tggactgccc cgcgtgtagt catagtgtcc tccgtacaat gtgatgaggg tggggagatg
     3721 cggtttgttt tcgatgaagg ggtgccagac cgtcgtgtgc gattggccaa aatcacagga
     3781 tccactagct cgccgcattg cacacaacgt tgggctgcag tcgcgtgctc actgttggaa
     3841 aggtccatgc agtattcttc gaccaggagg ccgccacagc gagtgcagcc gccggcttca
     3901 cgttcgcctt gctgtatgtg tgctataggc atgacccctc cctcctagat gttcaatgtt
     3961 tgttcagtgt atgtcatatg tataccgcaa cattgtccaa tgtgtcaata gcctctatgg
     4021 acaaatattt gacaaacatt tgacaatgcg tttagaatcg ataacagcgc atgaacactc
     4081 atagaattca tagggttgca aagctggttg gcctgtcgaa ggatgtgata cgcgtctggg
     4141 agaggcggta tgggcttgtc aaaccatcga gaagtaacaa ccgctatcgt gaatacacgg
     4201 atgaagacat agcactcctg cgatttctca aggaagaagt ggctcgcggt caaacgatcg
     4261 gcgggctggc agtagaggga cgggacgctg tgctacaacg catgaggacg acttcagccc
     4321 cgactccgca ggaactggag ccgcacgaac atctgctcga tgagctcgcc tcgttgctcg
     4381 agccgctgga aaaaacgcgt ttcgagcaga aacttaacgg cgccgtggcg gtgattccgt
     4441 tcgaggaggc cgttcagcga atcttgctcc cgctgcagcg gcgggtcggc gatttatggc
     4501 atgaaggcaa gctcaacgtc gcggtcgaac actatgtcac aaaactcatc cagcagaaat
     4561 tattttcggt gatgaatcaa ctttctatca acgagtccgg cccacgcatt gtcgtagcgt
     4621 gcccggaggg ggaatcacat gagctcggcg cgcaagctgt tgcctacttg gccgccgttc
     4681 gcggatgtca cgtgtattat ctgggcccca atcttcccgc ggtcgagctt gcggcattct
     4741 gcgagcgcgt gaggccagat ctcgttctgc tttcgctcac ggaaattacc tcggatcaga
     4801 ctgctcgccg tcttctcgat gagcttgggt ctctcgctgt caagtggcct gtcgctatgg
     4861 gcgggcaagg cgcacgcacg attgatcatc atttactcaa taaatacaag atcggactcc
     4921 tcgatgacct cactgcactg cacaaccgca tcatgaccct tatttcaaat cgcatgctgg
     4981 cttcaaggcc atgacacgca gttgtaaccg catcggcctc actcttttgg cagcctgacc
     5041 tggcggaacg gcatccaacc tggctgaacc gccttctcta tcacttagtg gagtcagccc
     5101 agggcgggcc aatggcagtg ttgaagactg gcctccttgt caggagactc cttaatctaa
     5161 cgactgattt gtccttggtt gatgtgacga taaagcaaat cagctattgc atgatgcccc
     5221 acagaattga agtgtaaggg atcattaaat agatagatgt ctcgcgaggg aagcctattt
     5281 acattcaaat actccaaaag ctcatcataa gggtcataga tcgttactcc cgccctacgt
     5341 ccagcttcct tgataatatt ctgcggttca agaatttcaa atgaccctgt tctgagctga
     5401 tactcaaaaa gaaacccccg ctggaaaggg agctgccact aaggctactg ccacgggccc
     5461 ccaccatcaa atagagcaat gattagcaaa acctccgaag atccatccat ctgccatcgt
     5521 tatcacactc atgaatgtga ctattgatgc aaacttgaga tcctggagga tacgatttgc
     5581 cgttgtgagt gcattgttta atctcggcgc tatctgaaat agtggttccg actaagggta
     5641 aaaaagcttg agacgctagg attgccaagc ctacaacaat ttgaaatatt cgtttcatat
     5701 ctcctccttt ggatgggcgg cttgtttatg gggggagctg ctgcatcccc ccaatgaaag
     5761 cagctgcgat acatgattag ttacgacagt acgtattcta ggtgagttgc tcggaaaggt
     5821 tcaagaaaca aacggcggca atccgtacaa gacaaacgcc aggcccatct cttttcaagc
     5881 ttcctaacga agggacccag aaaaagaaac cgccccgtcg cctaccagaa acgcatacgg
     5941 attattctag ggccttttat acttactcaa gctgaattga gaggttgtga acctgatctt
     6001 tatatgacct atcatatgga ttcttctgga aatcatgacc acacgagacc acaatcaaca
     6061 ccgttacgtc tccagtacct cccaaagcga tcctccactg ctgactctga ttaatctgta
     6121 cgcagtcctg cgacttttct gctagaagcg gcgccggtca tgcttgtctg catgacaagg
     6181 aggtcaactg tgaatggtct tctaccaatc gggataaggg attcggaact ttgaattaga
     6241 caccctaccg ctgctgcttg ccttatgggg caatccgcgc gccgttagat ggcacactta
     6301 tatcttgtgg cggcgcgttc ccattttgtg cgctcaaaca cgaacgtcgc acaagccctg
     6361 acaaaagagc tacagactgg gaatatatga caatccctag ggacaatggg aacagccagg
     6421 ttgcggaccg cgctactgct ggcgctctct gcgcagtcgc agcggatggg aggcattgca
     6481 gggggtcaca gggatgaagt acctcgcgtt cgcagtgacc gttggtgcca
//
LOCUS       DASSCQ010000134         7550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_124418, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000134 DASSCQ010000000
VERSION     DASSCQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124418"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFH05_09245"
     CDS             <1..298
                     /locus_tag="VFH05_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678444.1"
                     /translation="DGGGLGKGGTGTLYVNDRKVAEGRIERTQPMVFSADETADVGID
                     LASPVVKAIGSEARSKFTGTIRKVTVEVQEIEKADAAKERIARRAFVHKKMLSD"
     gene            366..1391
                     /locus_tag="VFH05_09250"
     CDS             366..1391
                     /locus_tag="VFH05_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678445.1"
                     /translation="MFSLRTPTVPSVFTLGISGFVFLIALARPVHAEVGVNGSATLFW
                     TDDIGIFSATRRLSRDGDPTQPALDTRLTDKGSSMVFEPQLTVSDSFTNGLGILHVDL
                     MGQGFIFSEDARFNQGMFRIQAVQSFSPQTRLRLRYSFVPDQFLGENEDRHPGQLGLV
                     DERVTSHIWSVRMEHAIMEDLEVKLLGRYGLRRYNEAFAERDTNFFTIGPHVDWRLSR
                     RVTLGLGYHYERGLADGRNEPELKDDVSYVNHYMTAELDIDLMDRWSLLMAAHYERNN
                     WTSGIPGDERKGQHENIVQGEMILVREVTDHIRIFGGFQRSNRKESFETDAVKNTNLG
                     FGISGSF"
     gene            complement(1821..1898)
                     /locus_tag="VFH05_09255"
     tRNA            complement(1821..1898)
                     /locus_tag="VFH05_09255"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1860..1862),aa:Glu,seq:ttc)
     gene            2030..2512
                     /locus_tag="VFH05_09260"
     CDS             2030..2512
                     /locus_tag="VFH05_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678446.1"
                     /translation="MQGRNLTWFVTRVICCFLLTACAHDTYQQRADVIKDHTEAFYTH
                     LKANRVEAAIRENEQIEAMASDMGRTVRKRASLQGATPVEREFALMKTAKEAAATNWL
                     ALGQYFAIKRQYPQARATYQRILHTYDHETDRPYREQAARAIEDLNILDPPSASATNP
                     "
     gene            2509..3057
                     /locus_tag="VFH05_09265"
     CDS             2509..3057
                     /locus_tag="VFH05_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678447.1"
                     /translation="MSVQDSRDALSQVFALCLLFLGACAHSIHVTPVPPSVSTSPIDH
                     ALQVQVPLLALEGADRMPGVIMLDWPVSDLRQAVIEYARQRRTFTEVHDEHGDYTLAV
                     HAWLWLRSREAYRYIVHLEADLGPTGQPPKKSYVVEKETVGSRIRWVTTSDQAPIAAA
                     VQAVLDELFDHIEQDAAVFGKQ"
     gene            complement(3071..3283)
                     /locus_tag="VFH05_09270"
     CDS             complement(3071..3283)
                     /locus_tag="VFH05_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678448.1"
                     /translation="MKRFPWTSSVASEEWLGAESHLPNLKQELRNQNHRGLNTAEVVV
                     AGALFTAGLTLGTLAALWLTRSRRTG"
     gene            complement(3342..4037)
                     /locus_tag="VFH05_09275"
     CDS             complement(3342..4037)
                     /locus_tag="VFH05_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET6678449.1"
                     /translation="MAELSVLNQPAKPVPKDRLPMPVQLAAGLEKLSLALKSRTWRRE
                     GQAGLGPLQRQVLTLLRSKADHSAQVSAIAQELVVRLPTASEAVATLERKRLVKRFRS
                     HRDGRIVNVELTARGARACAPAVGIPNLLAEATSALTSTEQVAFLKGLMKVIRTLQQQ
                     GEISVARMCVSCRFFRPHQYAEAAQPHHCDYVNAPIGDHALRLDCAEYEPASISQAKE
                     SWDRFNESRTAIG"
     gene            complement(4227..4430)
                     /locus_tag="VFH05_09280"
     CDS             complement(4227..4430)
                     /locus_tag="VFH05_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678450.1"
                     /translation="MASDPAVLIRVCSLCQLIHDDGETAYERWVTRDVYKMANGVDPP
                     KSRLTYTYCPDCFSESADPLKAA"
     gene            complement(4543..5373)
                     /locus_tag="VFH05_09285"
     CDS             complement(4543..5373)
                     /locus_tag="VFH05_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678451.1"
                     /translation="MTGDYSAADLCRILRLDASQLRACLRAALLPPPGVGRSCTYSFQ
                     NLVVLRTAKGLTDAGVSVAKIRKVLECLERQLEEDTSIASVKIYASGKRVVVWDGASR
                     WQPDSGQFLLNFETAQMLPPRPLRSAPQVQRTERETAERWFQRALTLVEDSPEEARQA
                     YQEAIRLHPGFTDAHVNLGLLNHNLGLLADAEACYRRALEHEPKLALAHFNLGVLLED
                     RHQKSAAISAYKEALVHAPEFFEAHCNLAQLYEKLGRKRDAFRHYAAANRIQGRGINQ
                     "
     gene            complement(5370..6224)
                     /locus_tag="VFH05_09290"
     CDS             complement(5370..6224)
                     /locus_tag="VFH05_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET6678452.1"
                     /translation="MGRRLETAHISFGLVTIPVGIYSAIDEQDIHFNQLHAVCGSRIK
                     QQRFCPVCQREVKYEELTKGYEITKDEYVRIDQEELDRLQAAESEAMQILEFVPLSAV
                     DPIYFESTYYLGVEKQGEKAYQLLAQAMEDMQRVALAKYVWRGKDSLHVIRSVEGRLL
                     LHRMYYHDEIRPFIEVPKSDQKPASAELKLATQLIDSISTDRFDAGAYQDEYRQRVIE
                     LIEQKSKGKTVTLAPKAAAPATDVVDLMQRLKESLAQSAQKKTARSRALPAQTAREPA
                     KKASSGRR"
     gene            complement(6424..6954)
                     /locus_tag="VFH05_09295"
     CDS             complement(6424..6954)
                     /locus_tag="VFH05_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6678453.1"
                     /translation="MSLFGSSNPVFEMLERDHAKVKDLFEQFEDAQDARSRERIIQET
                     LLELDVHAKLEETLIYPAIRKKLDEEDIMDEALEEHHVAHVLMNELKRMRPSDRRYHA
                     KFKVLGENIKHHVKEEEGTMFPKAEKVDLDWEKLEQKANEQKEALMARRMNGTKRDRT
                     KKPRRKTARRPLRRAA"
     gene            complement(7281..7355)
                     /locus_tag="VFH05_09300"
     tRNA            complement(7281..7355)
                     /locus_tag="VFH05_09300"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7321..7323),aa:Cys,seq:gca)
BASE COUNT         1638 a         2066 c         2103 g         1743 t
ORIGIN      
        1 cgatggcggc ggactgggta aggggggaac tggaacgctg tacgtcaatg atcgcaaggt
       61 tgccgaaggt cgaatcgagc ggacgcagcc gatggttttt tcggccgacg agactgccga
      121 cgtgggcatc gatctggcct ctccggtggt caaggcgatt ggttcagaag cgcggtcaaa
      181 gttcaccggc acaattcgta aggtgactgt cgaggttcag gagatagaaa aagcggacgc
      241 agccaaagag cgaatcgctc ggagagcatt tgtacacaag aaaatgctgt ccgattgagt
      301 gcacgagata gggatatctg ctttgttctt gacttgatga atgttgcaga gctttccaag
      361 ctttcatgtt ctcactgaga acgcctaccg tcccgtcggt ctttacgtta ggcattagcg
      421 gattcgtctt cctcatcgcc ctcgcgaggc ccgttcacgc ggaagtaggt gtcaatggct
      481 ccgcgacgct cttttggaca gatgacatcg gcatcttttc agcgactcga cgcttatcgc
      541 gtgacggtga tcccactcag cccgcacttg atacacgcct gacggacaaa ggttcctcca
      601 tggtcttcga gccacagttg acggtgtcgg actcattcac gaacggcctg ggcatattgc
      661 atgtggatct catgggacag ggatttatct tctcggagga cgctcgtttc aatcaaggaa
      721 tgtttcgtat tcaggcggtc caatcgttct cacctcagac gcggctacga ctgcgctaca
      781 gctttgtgcc cgatcagttt ctcggcgaaa acgaagaccg acacccaggc cagttaggcc
      841 ttgttgacga gcgcgtgacc agccatatct ggtcggtacg gatggaacac gcgattatgg
      901 aagacctgga ggtgaagctc ctcggtcggt atgggctccg ccgctacaat gaggcgtttg
      961 cggagcggga taccaatttc ttcaccatcg gccctcatgt ggattggcgt ctctcgaggc
     1021 gcgtgacgct gggccttggg tatcactatg agcgcggctt agcagacggc cgaaacgaac
     1081 cggaacttaa agacgatgtt tcgtatgtga accactatat gaccgcggag ctggatattg
     1141 acctaatgga ccgctggtcg ctgctgatgg cggcccacta tgaacgaaac aattggacga
     1201 gcggcattcc tggagacgaa cgcaagggac agcatgagaa catcgtccaa ggagaaatga
     1261 ttctcgttcg ggaggttacc gaccatatcc ggatctttgg cggctttcag cggtccaatc
     1321 ggaaggaaag ttttgagacc gacgccgtga agaacacgaa tctcggcttt gggatttctg
     1381 gttcttttta atacgcttcc gtgggatggg gggggaagtt agtcaaaata gcctcattaa
     1441 gtagctccat tttaaccggg aatgatgaac ctgtttgagg tagcgtatga actcaggagc
     1501 gcctgttcga gattgcccct cgcttgctgc cagtcaatcc aatcgattgc tggcttcttt
     1561 gcagagcgga tggcgcggcg aaaacgattg gaacatcctc tatatctacg gagcgctcaa
     1621 tttttgaagc ggtgaacaaa cccgcgactc tttattactt tgcacaatcg ccaagtgggg
     1681 ccgtcccgtc agattctccg gaagctcttc aatggtgaaa agattccctt caatcctgtg
     1741 acaagtgaca gcggatcgag gtatgagttt cagggtacag ccgtccgtta gcaggcttct
     1801 tgtagggcgt gcaaaggagg tggtgtcccc aacgggattc gaacccgtgt tgccggcttg
     1861 aaaggccggc gtcctaggcc aggctagacg atggggacct aaggtgagca agaagggaaa
     1921 cacataccac actcaagatg ggcctgtcac tacaacctag agacggtcag tcgatatttc
     1981 ataatcgatc aggtaggatg agtcctcgtc ggctgagagg tcgagagtca tgcaaggaag
     2041 aaacctgact tggtttgtca cacgtgtgat ctgctgcttt ctgttgactg cctgcgcaca
     2101 tgacacgtat cagcagcgtg ccgatgtcat caaggatcat actgaagcct tctacacgca
     2161 cctgaaagcg aaccgtgtgg aggcggctat ccgtgaaaac gaacagattg aagccatggc
     2221 aagcgacatg ggccgcacgg ttcgaaagcg cgcctcgctg caaggggcga ccccggtcga
     2281 gcgagagttt gccttgatga agacggccaa agaagctgcc gcaaccaact ggctcgccct
     2341 cggccagtac ttcgccatca aaaggcagta cccacaggcc cgggcgactt accagcgcat
     2401 tcttcacacc tacgatcatg agactgaccg cccctaccga gagcaagcag cccgcgcaat
     2461 tgaagacctt aatattctcg atccaccgtc cgcgtcagcg acaaacccgt gagtgtgcag
     2521 gacagccggg atgcgttaag ccaggtgttt gccctgtgtc tcttgttttt gggcgcctgc
     2581 gcacacagca ttcatgtcac acccgttccg ccctccgtct caacctcccc tatcgaccac
     2641 gcgctgcagg tacaggtccc gttgctcgca ctggaaggcg cagaccggat gccaggcgtc
     2701 atcatgctcg actggcctgt gagtgacctt cgtcaggctg tgattgagta tgcgcggcaa
     2761 cgtcgtacgt tcaccgaggt ccacgatgag cacggggatt acacgttggc cgtccatgca
     2821 tggctctggc tgcgttctcg agaggcctat cgctatatcg tccatctcga agcggacctc
     2881 ggcccgaccg gtcagccacc caagaaatcc tatgtggtcg agaaagaaac agtggggtca
     2941 cggatccgat gggtcacgac ctcggatcag gcgccgattg cggcggccgt gcaggcagtc
     3001 cttgacgaac tattcgatca tatcgagcag gatgcggcgg tgtttggaaa gcagtagcga
     3061 gcgaagcgcg ttagccggtg cgtcgcgaac gagtcaacca gagggccgca agcgttccca
     3121 aagtgagtcc cgctgtgaac aaggcccctg ccaccaccac ctcggcggtg ttcaagccac
     3181 gatggttctg gtttcgcaat tcttgcttga ggttcggcaa atgcgattca gcgccgagcc
     3241 attcttcgga agcgacggaa gatgtccagg ggaaacgttt catgcctcgg tccttttcca
     3301 tgtgatgaac cacaactcct ctcgtcatgg cagcttcgca attagccgat cgctgtccgg
     3361 ctctcattga atcggtccca cgactccttg gcttgtgaga tggaagcggg ctcgtactcc
     3421 gcacagtcca accgcaatgc gtggtcgccg atcggcgcat tcacatagtc gcagtgatga
     3481 ggctgagcgg cctctgcgta ctgatgtggg cggaaaaacc ggcaggacac gcacatccgt
     3541 gccacagaga tttctccttg ttgttgcaag gtccggatca ctttcatcag gcctttcaag
     3601 aaggccactt gctccgtgga cgttaacgcg ctggtcgcct cggcaaggag attgggtatt
     3661 ccgactgccg gggcacaagc ccgcgcgccc cgggccgtca actcgacatt gacgatccgg
     3721 ccgtccctat gcgaacgaaa gcgcttcact aatcgcttcc gttcaagggt ggcgacggcc
     3781 tcggacgccg tcggtaaacg tacgaccagt tcttgagcaa tggcggagac ttgcgcgctg
     3841 tgatcggcct tgcttcggag caaggtgagc acttgccgct gcagaggacc aagcccggct
     3901 tgcccttcac gccgccacgt cctacttttc agcgccaggc tgagtttctc gagcccggcc
     3961 gcgagttgaa ccggcatcgg caggcggtct ttcggcaccg gtttcgccgg ttggttcaat
     4021 acgctcagtt cagccatggc agccccttag agaaccctct tagcgagtgt agtgggtcga
     4081 ggaaactccg tcaagcccca attgccatga ggaaatccca cggcgaatgc tcactcagcc
     4141 gatgatgacg aaagtttcga ctgaagagcg gaacaaccgg agctgatgac ggcgctcaac
     4201 tgacaagaga aacccgccga tgcgggctag gcggctttga gaggatcggc agattcgctg
     4261 aaacagtctg gacagtaggt gtacgtcagg cgtgatttcg ggggatcgac gccattcgcc
     4321 attttataaa cgtcacgagt gacccagcgt tcataggcgg tttccccatc gtcatggatg
     4381 agctggcaga gactgcagac gcggattaag actgctggat cagaagccat gggatccttt
     4441 catggcccag cataaaggac agcccccgtt cgatcattgg gatttcccct agggagtggc
     4501 tcagaagcca cctagtgcgg ggcgagaacg atcctaaagg cttcactgat tgatgccccg
     4561 accctgtatc ctgttggccg cagcataatg gcggaaggca tctcgttttc gcccgagctt
     4621 ttcatacaac tgtgccaagt tacagtgcgc ctcaaaaaac tccggcgcgt gcaccaaggc
     4681 ctctttgtac gccgaaatag ccgcgctttt ctgatgacga tcctccaaca gcacgccgag
     4741 gttgaagtga gccagagcaa gtttgggttc atgctccagg gcgcgacgat aacaggcctc
     4801 agcatcggcc aacagaccga gattgtggtt tagcaggccc agattgacat gcgcatccgt
     4861 aaaaccaggg tgtaaccgaa tggcctcctg ataggcctgt cgggcctcct caggagaatc
     4921 ttccaccaag gtcaaggctc gctgaaacca tcgttcggcc gtctctcgtt cagtccgctg
     4981 gacttgaggc gctgaccgta gcggcctggg cgggagcatc tgtgcggttt caaaattgag
     5041 aagaaattgg ccagaatccg gttgccagcg cgaggcacca tcccacacga ccacccgttt
     5101 gcccgaggca taaattttta cactggcgat ggaggtatcc tcttccagtt gccgttccag
     5161 gcattccaga acttttcgga tcttggccac cgacacgccc gcatcggtga gacctttcgc
     5221 cgttcgaagc acgacgagat tctggaatga gtacgtgcac gatctgccta cgcctggtgg
     5281 cgggaggaga gcggcgcgga gacaggctct caactgggac gcatcgagac ggaggatacg
     5341 gcagagatcg gctgcggagt agtctcccgt catcgtctcc cggacgaagc tttcttggct
     5401 ggttcacgag cagtttgtgc gggtagagcc cgcgatcgag cggttttttt ctgcgcagac
     5461 tgcgcaagac tttccttcaa ccgctgcatc agatccacga cgtcagtggc gggggcggca
     5521 gccttgggag ctagagtgac cgtcttccct ttggatttct gttcaatgag ctcaatgact
     5581 cgctgacgat attcgtcttg ataggccccc gcatcgaatc gatcggtgga aatcgagtcg
     5641 atcaattgtg tggcgagctt cagttcggca gaagccggct tctggtcgct cttcggcact
     5701 tctataaagg gccgtatttc atcatggtaa tacatcctat gcagcagtaa acgaccctcc
     5761 actgagcgga tgacatgcag gctgtctttt ccccgccaga cgtattttgc gagagccacc
     5821 cgctgcatat cttccatcgc ttgcgccaac agttggtacg ccttttcgcc ctgcttctcc
     5881 actcctaggt aataggtgct ttcgaaatag atcggatcga cggcgcttaa cggaacaaat
     5941 tccaagattt gcatggcctc actttcggcc gcttgcaacc ggtccagctc ttcctgatcg
     6001 atacgaacat attcatcctt cgtgatttca taaccctttg tgagttcctc gtacttcact
     6061 tcccgttgac agacaggaca gaagcgctgt tgcttgatgc ggctgccgca gactgcatgc
     6121 agttgattga aatggatgtc ctgctcgtcg atggcagaat aaatcccgac gggaatggtc
     6181 acaagcccga acgaaatgtg tgccgtttca agccgacgtc ccatcgtccg tagagtgcgt
     6241 cgcccgaatc gcgagggata ctaggagaac ccctaggtgc cgggccttgt acgacattcc
     6301 cgcaatcagg tgctagtggt acgtttttgt gctgctgtgc cggctaggac cgtatgttaa
     6361 atgagcgtta gatgatgcga ccgggccgag ccggcgcagc gccgcgcacg gattcgtcaa
     6421 cccttacgcg gcccgtcgca gaggcctccg agccgttttt cttctcggct tcttggtgcg
     6481 atcgcgcttt gtgccgttca tcctcctggc cataagcgct tccttctgtt cgttcgcttt
     6541 ctgctctaat ttctcccaat ccagatcgac cttttctgcc ttcggaaaca tcgtgccttc
     6601 ttcttctttg acgtggtgtt tgatgttctc tcctaagact ttgaacttcg catggtatcg
     6661 acgatcgctg gggcgcatcc tcttcaattc gttcatgaga acatgggcga cgtggtgctc
     6721 ctccaaggct tcgtccatga tatcttcttc atccagtttc tttcgaattg ccggatagat
     6781 gagggtttct tctaattttg catggacgtc caactcaaga agcgtttctt gaatgatccg
     6841 ttctctcgaa cgggcatcct gcgcgtcttc gaattgttca aataagtctt tgaccttggc
     6901 atgatccctc tccaacatct caaacaccgg attggacgat ccgaataatg acataatccc
     6961 tccttactct acggacgtga ttcgagtgac tctgccttaa gtaagaccac tagctcatcc
     7021 tatgcgtcaa tgaaatgaca ccgaactagt gagttccctt gcccgagaga tccttgttag
     7081 cgatgatctt cttcgaagtc cgtggttggc tacgtaggat taagttcgca gagaggggac
     7141 acgaaagtag atgcggtatt gaacagttct agactatcga ctcgtctgcc ccccttccga
     7201 ccaagcgctc tgactctcga ggaaaggttt atttattgga cactaatgct cgcggctttt
     7261 ggctggcagt gatgctgaag tggaggcgcc gagcggaatt gaaccgccga atcacagcgt
     7321 tgcaagctga gcctttggcc acttagtacg gcgccgagcc tagaatatgt gaatgcaagt
     7381 cggtgccggt actggttgtt cccctagcca cgaataccat tcgcttttcg agtggaaggc
     7441 aggcagagca gtcgcgagaa ctgggggacg actccccgta tacattttac ctcgcgcgaa
     7501 atttaacgcg aaatttacag atcggtggac actttgtcat ttctaaaaga
//
LOCUS       DASSCQ010000135         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_984230, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000135 DASSCQ010000000
VERSION     DASSCQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984230"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="VFH05_09305"
     CDS             <1..1754
                     /locus_tag="VFH05_09305"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678454.1"
                     /translation="DDSKFEQARHAVNRQLVEQLGKQRGSFSGLLHPVQISFDNSQSP
                     SDTIMDIRSDDTPAFLYAFANALAMRNVYIARAQITLEGGKLHDRFAVRNRYGQKFID
                     PVDQQQLRLTAVLIKQFTHALTWAPDPTKALEAFDQFLDLTVQETKGKAREEALAFLG
                     DKKTFPLLARLLGASDFLWEDFLRRQHDHLLPLLQDYRDAPLIKPQKVLRKALDRVVE
                     RHKDEAGRKEALNRFKDQELFRIDMKHIVAPDSSLADFSLALTELAEIIVQRSLKDCQ
                     AKLRKQYGSPRLANRKPCPFAIMGAGKFGGRELGYASDIEVLFIYGGAGRTDGKDRIE
                     NSEYFERLSQELLQWIETKQEGIFHLDVRLRPHGGKGTLANPLDEITAYYSDKGLAAP
                     FERQAMIKLRHVAGDEALGKQVEAHRDRYVYSGAPWDLTAALDLRRQQLKQLVERGTV
                     NAKLSHGGVVDIEYAIQYLQLMHGHRHAVLRTPNAMQALAGLIESGIVSRHDGENLRK
                     AYLFLRMLIDGLRMVRGHAKDLVIPPADSEEFIFLARRVGYTTDDWQAGARHLKTDID
                     EHMGRVKEFWERTFGKL"
     gene            2171..2497
                     /locus_tag="VFH05_09310"
     CDS             2171..2497
                     /locus_tag="VFH05_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678455.1"
                     /translation="MRIVLICLLTLILSGCINITTELQPSNTEVQSILLGEDCTPVVL
                     GVGLGTNTIEGAKKDGRPMGDVGEFRSRVKLNDRTVINRIRTIQITNFYVLLVGSSCI
                     QVVGEP"
     gene            complement(2624..>3405)
                     /locus_tag="VFH05_09315"
     CDS             complement(2624..>3405)
                     /locus_tag="VFH05_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6678456.1"
                     /translation="ITRADRLRVVTFEQHRESLDPRATLRRSLVPAGDAVVYQDRSIH
                     VISWAKRFLFKPEQLDLPVSRLSGGEQARLLIARLMLQPADLLILDEPTNDLDIPTLD
                     VLEDSLLEFTGALALVTHDRWLLDRISTRLLALDGTGCAQWFADYAQWEAAQSRNAGD
                     ARRSAPADVVNAVKAPSKRKGLSYREQQEWEKIEEQILKAEEIVAACQTAANDPAIAS
                     SAGTLQERYAALHTAQADVERLYARWAELDEKRAQAVKSAS"
BASE COUNT          727 a          961 c         1017 g          700 t
ORIGIN      
        1 tcgatgacag caagttcgaa caagcccggc atgcggtgaa tcggcaactg gttgaacagc
       61 tcggcaaaca gcgcggctcc tttagcggtc tgctccatcc ggttcaaatt tcattcgata
      121 acagccagtc gccgagcgat accattatgg acatccggtc ggacgacacg ccggcatttc
      181 tgtatgcctt cgcaaacgca ctcgccatgc ggaatgtcta tatcgccagg gcacagatca
      241 cgcttgaagg cggcaagctg catgaccgct ttgccgtcag gaaccgctac gggcagaagt
      301 tcatcgaccc ggtggaccag cagcaattgc gcctgaccgc cgtcttgatc aagcaattca
      361 cgcatgccct gacctgggct ccggacccca caaaggcctt ggaagcgttc gaccagtttc
      421 tcgacctcac cgttcaagag acgaaaggaa aggcgagaga ggaggcgctc gcctttctcg
      481 gcgacaagaa gacgtttcct ctgctcgccc ggctgttggg cgccagcgat tttttgtggg
      541 aggattttct ccggcggcag catgaccatc tgttgcccct gcttcaggat tatcgcgatg
      601 cgccgctcat caaaccgcag aaggttctac ggaaagcgct tgaccgtgtc gtggaacggc
      661 acaaagatga agccgggcgt aaggaggcgt tgaaccgctt caaggatcag gaattgttcc
      721 gcatcgacat gaaacacatc gtcgcgccag atagcagcct ggctgacttt tcgctcgcgc
      781 tgaccgaatt ggcggaaatc atcgtccaac gcagtctgaa agactgtcag gctaagttgc
      841 gcaaacaata cggctccccg cggcttgcga acaggaagcc ctgccctttt gcgatcatgg
      901 gcgccggaaa attcggcggg cgggagttgg gctatgcctc cgacatcgaa gtcttgttca
      961 tctatggcgg cgccgggcgc acggacggaa aggaccgcat tgagaacagt gaatactttg
     1021 aacggctcag tcaggaattg ctgcaatgga tcgagaccaa acaggaaggc atcttccacc
     1081 tggatgtacg cctccggcct cacggaggca aaggtactct cgccaatccg ctggacgaaa
     1141 tcacggcata ttacagtgac aagggactgg ctgcgccgtt cgaacggcag gccatgatca
     1201 aactgcgcca tgtcgccggc gatgaggcac tcggcaaaca ggtcgaagcc catcgcgacc
     1261 ggtatgtcta cagcggcgcg ccctgggatc ttacggcagc actcgatttg cgccgacagc
     1321 aactgaaaca gctcgtcgag cgcggcacgg tgaacgcgaa actcagccat ggcggcgtgg
     1381 tggatattga gtatgccatt cagtatctcc aactgatgca tggccacagg catgcggtgt
     1441 tgcgcacgcc gaatgccatg caggcactgg ccggactgat tgaatcgggc atcgtcagca
     1501 ggcacgacgg cgagaacctt cgaaaggcct atttgttcct ccgcatgctg atcgacggac
     1561 tccggatggt gcggggtcat gccaaggacc tggtcatccc gcctgccgat tcagaagagt
     1621 tcatctttct cgcacgccgt gtcggctata cgacagacga ttggcaggcg ggggcgaggc
     1681 atttgaagac ggatatagac gagcatatgg gaagggtgaa ggaattttgg gagcggacgt
     1741 tcggtaaatt atgaggcagc tggcctggtc ggtccctgtg ctcgccgaac gcgacacacg
     1801 ggaaccattg gatgatcaca ctgcgggagg tgttcgttca ggcgcgcacc atgggaccga
     1861 tccagccgct tagggccttt catccggtga ggtggaggga tatcagcaag ctcggtggga
     1921 gcatcatatt gatgcgagcc gtcgaagtcg ccgtccgacc caaatcctcc ccatccctcc
     1981 tcagagagga acgaacgagc gtgctttgaa ggagcattag atttaaataa gcccaccatc
     2041 cacacagccg tagagagggc gggctcagga tggcaactac aggtagcctt tcaatcgaat
     2101 tgacacactc gttctcgcgc agtaccctgt ctggcttttc tcgcttccct tttaagaagg
     2161 agtcggtctg atgcgaattg tgctcatctg tctgttgacg ctgatcctgt ctggttgcat
     2221 caatatcacc acggaactac aaccgagcaa taccgaggtc caaagcatcc tgcttggtga
     2281 agactgcacg cccgttgtcc tcggcgtggg actcggcaca aacaccatag aaggcgcgaa
     2341 aaaggatggc aggccgatgg gagacgttgg cgagttccgc tcgcgtgtaa aactcaatga
     2401 tcgaacggtg atcaaccgaa tccgcacgat tcagatcacc aacttttatg tattgttggt
     2461 tggttctagc tgcattcaag tagtcggcga gccgtgacca tctcttccat tcctccggtc
     2521 gactttagcc gactccgccg tccatgacgg acaccttccg gtgaactgat cgctttcgcc
     2581 ttgcgatttt ttgaaggact ggccacagtc cattggcccg gtactaggac gcgctcttca
     2641 cggcctgggc acgtttctca tcgagctcag cccagcgtgc gtaaagacgt tcgacgtcgg
     2701 cttgtgcggt gtggagtgcg gcgtaccgct cctggagagt tccggcagac gaagcgatgg
     2761 ccggatcgtt cgctgccgtt tgacaggccg cgacaatctc ctcggccttg agaatctgct
     2821 cttcgatctt ttcccattcc tgttgctccc gataggacaa ccctttccgt ttggaagggg
     2881 ccttcactgc gttcacgaca tctgcggggg cagatcgtct ggcatcgccc gcattccgag
     2941 actgtgcggc ttcccattgg gcatagtcgg cgaaccattg ggcgcagccc gtgccgtcca
     3001 gcgcgagaag ccgggtggaa atgcggtcga gcagccaccg gtcgtgcgtg acgagggcga
     3061 gcgctccggt gaattccaac aggctgtctt ccaacacgtc gagcgtcgga atatccaagt
     3121 cgttcgtcgg ctcgtccagg atcaggaggt cggccggctg gagcatcagt cgcgcaatga
     3181 ggagtcgggc ctgctccccg ccggacaggc gggagaccgg tagatccagt tgttccggtt
     3241 tgaagaggaa ccgctttgcc catgagatga catggatgga ccggtcttga tagacgacgg
     3301 cgtcaccggc cggtacgagg gagcgtcgca gcgtggctcg cggatccagc gattcgcggt
     3361 gttgctcgaa ggtgacgacg cgcagccggt cggcgcgtgt aatgg
//
LOCUS       DASSCQ010000136         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_486740, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000136 DASSCQ010000000
VERSION     DASSCQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486740"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..93
                     /locus_tag="VFH05_09320"
     tRNA            16..93
                     /locus_tag="VFH05_09320"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Glu,seq:ctc)
     gene            complement(148..510)
                     /locus_tag="VFH05_09325"
     CDS             complement(148..510)
                     /locus_tag="VFH05_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678457.1"
                     /translation="MTRLAPETPLFWSVWGRRSIGMHRAPMTGKNYLAAVQALWASHR
                     LSDAKASRHPSWCVAMEVLEQRHDLEQVRALLGHQRIDTTQIYTMIRPAQLKQAVSFY
                     EEPARRIGERRKRPQIHV"
     gene            1152..2261
                     /locus_tag="VFH05_09330"
     CDS             1152..2261
                     /locus_tag="VFH05_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET6678458.1"
                     /translation="MTGGREFHDGAKDGLEALEPLNPEKVRSFDDLLIAMRKTAFGGR
                     RLGEAYEVLWAMIEDPDCAVVMTLSGAMTIAKMGKIISSMIDYGMVQCVVSTGALIAH
                     GLSESVGKTHYRVPPSVSDEELFKKGYNRVYDTLEMEANLNYVEHVVGQTMKRITPEQ
                     TLSSEMLTRELGKTLTEEFDGDGILKSAYLKKVPVYIPAFTDSEMGLDVGTWAMGRVV
                     DQARAQAKGGSDLDILRIINKSYPSFNPYQDLNSYTALITSAKRLGIFTIGGGVPRNW
                     SQQVGPYIEISNMRLGLSVKPPRFQYGVRICPEPDYWGGLSGCTYQEGVSWGKFVPPE
                     EGGRFAEVLSDATVVWPLLMAGLLERTKAKQPART"
     gene            2258..2896
                     /locus_tag="VFH05_09335"
     CDS             2258..2896
                     /locus_tag="VFH05_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6678459.1"
                     /translation="MKGTTVLRSALLLLVCGLLLGASGTGPVTDDGRVQGKPDAPLTL
                     IEYSDFTCGYCLKFFQETLPRLQAAYLDTGKVKFVYRDYPRSDRGVGVEAAVAARCAG
                     AQGRYWAMHDRLFGEGRRLDSGSFKRVAKSVGLDEMQFGKCFDERRYLESIFQDRQEA
                     NRWGFHGTPGFILMQTVAGPTEKVPAVAIPGAFPFEAFAEEIDRMLSKVQGS"
     gene            2965..3234
                     /locus_tag="VFH05_09340"
     CDS             2965..3234
                     /locus_tag="VFH05_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678460.1"
                     /translation="MSDTQSFWSVPQRDGEPPFWVCMSCLSEAFYRKVPMPDCPNCHG
                     VSTYEAFAIEAIADWGTEDLVAKAKASEQAANVEPASASSVESST"
     gene            3261..4700
                     /locus_tag="VFH05_09345"
     CDS             3261..4700
                     /locus_tag="VFH05_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6678461.1"
                     /translation="MQGPRPFLVGGHWRQSDTRAPVHDPFTGKVLAEVSQGSEADAED
                     AIQSTAAAAQPMGALPSHARFHLLQRIAGALYDRREEFARIMTGEAGKPITDARREVA
                     RAVQTFTIAAEEAKRIPGEVIPLDWTPGMDSHLGILRRVPIGPVLGITPFNFPLNLVA
                     HKVAPALAAGNPILIKPAPQTPLTALLLAEVGLEAGVPPGGLNVLPCDNRVAERLVVD
                     PRFKLLSFTGSAAVGWMLKAKCGKKKVVLELGGNAGVIVEQDADLDFAAQRCASGGFG
                     YAGQTCISVQRIFVHHSVVDLFTTKLLLQVARLKGGDPNDEATVVGPLIDQHAAHRVE
                     EWIGEAVAQGARVLLGGKRMGSVVEATVLTNVTPTMKVSCQEVFGPVVTVTPYRQFDE
                     ALEARTRSEYGLQAGVFTQDVNKAFHAFRHLEVGAVLVNEIPTFRAEHMPYGGVKDSG
                     LGREGLRISIEEMTEPRFLVLNVRPPSSA"
     gene            4854..>4942
                     /locus_tag="VFH05_09350"
     CDS             4854..>4942
                     /locus_tag="VFH05_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HET6678462.1"
                     /translation="MFLTRGVGVHREKLASFEQALRSAGVAYC"
BASE COUNT         1043 a         1334 c         1474 g         1091 t
ORIGIN      
        1 attccttaca cctcgggtcc catcgtctag cctggcctag gacggagccc tctcaaggct
       61 cagacacggg ttcaaatccc gttgggacca ccaccttgaa attgttaggt aatttcgctc
      121 cgtgtttgag gaacattcaa atgttcctca aacatggatt tgcggacgct tccgacgttc
      181 tccaattcgt cgcgccggct cctcgtaaaa cgatacggct tgcttcaact gcgccggtcg
      241 gatcatcgtg tagatctgcg tcgtgtcgat ccgttgatga ccgagtaagg cgcggacttg
      301 ctcgagatcg tgacgttgct ccaacacttc catcgccaca caccatgacg gatgtcgtga
      361 ggctttagca tcggatagcc gatgagacgc ccatagagct tgcacagccg ccagataatt
      421 cttccccgtc atcggcgccc gatgcatgcc gatcgatcga cgtccccaca ccgaccagaa
      481 gagcggcgtc tctggcgcaa gcctggtcac gctaggtgcg acgatcacta cgaaggcagg
      541 gcgttggtca gaactcccgg aaagcgaaag agtggttcga taaagctgtg aaacaggggc
      601 atacgggcgc ccaagtcaat ctcggtacgc tgtatctccg cggggaaggc actcctcaaa
      661 gtagccaaat ggcattgttc tggtttacac gagcggcagg acaagaggat gctttggcgt
      721 tagcaaaact tgggttgatg tactcacagg gaaggggagc gcttcaagat ttcattcaag
      781 cccacatgtg gtacaacctg tcggcggccc gtggagaaac aagatttatc aatttcgctc
      841 gaacccttct gcggaagtat cctctcttca tgccgcacgc cctcgcatga gcgtgatgtc
      901 ccgcctcgaa tcgcatttcg acgtcgtatc tcgtattgga atggtgtctg tgggttgaag
      961 cggggtcacc tcgaagtcag tgtgttctga caaggtgccg ctctcagttt cgccagtggt
     1021 gtcccctaca agatccaggt gtccatgcat gctgctgatc cgcatcgtgc agctcttcat
     1081 tcgttgacgc ctcctgtatt ggttgtgtat aacggatgcg cttacacttc tcacattttg
     1141 cgggcagaca catgacgggc ggacgcgaat ttcatgacgg ggcgaaagat ggcttggaag
     1201 ccctggaacc cctgaatcca gaaaaggtcc gttcattcga cgacctcttg attgccatgc
     1261 ggaagacggc attcggaggc cggcggctcg gtgaagccta cgaagtgctg tgggccatga
     1321 tcgaagatcc ggactgcgcg gtcgtaatga cgctttccgg tgccatgacg attgccaaaa
     1381 tgggcaagat catcagcagt atgattgatt acggcatggt gcaatgcgtc gtgtcgacgg
     1441 gtgcgttgat cgcgcacggg ttgagcgaat cggtggggaa aactcattac cgcgttcctc
     1501 cctcggtgag tgacgaggaa ctatttaaaa agggatacaa ccgggtgtac gacacgctgg
     1561 agatggaagc gaatctcaat tacgttgaac atgtcgtcgg ccagaccatg aagcgcatta
     1621 ctcccgagca gacgttatcc tctgagatgc tcacgcggga gctgggaaaa acgctgacgg
     1681 aggagtttga cggggacggc atcctcaaga gcgcgtactt gaaaaaggtt cccgtgtaca
     1741 ttccagcctt caccgactcg gagatggggt tagatgtcgg tacctgggca atgggacgcg
     1801 tggtcgatca ggctcgtgca caagccaaag gcggaagtga tctcgacatc ctgcgcatca
     1861 tcaataagtc gtatccatcc ttcaatccct atcaagatct caacagttat acggcgctca
     1921 tcacctcggc caagcggctc ggcattttta ctatcggcgg gggagtgccg cgtaattggt
     1981 ctcaacaggt gggaccctat attgaaatca gcaacatgcg tttaggattg agcgtcaaac
     2041 caccgcgctt tcagtatggc gtgcgtatct gccccgagcc tgattactgg ggaggattga
     2101 gcggctgcac ctaccaggaa ggagtgtctt gggggaagtt cgtgccgccg gaagaaggcg
     2161 ggcgttttgc cgaggtactg agcgatgcca cggtcgtctg gccattgctg atggccggct
     2221 tgctcgagcg gaccaaggca aagcaaccgg cgcggacatg aaggggacga cggttctgcg
     2281 ctcggccttg ctgttgctag tctgcggact gcttctggga gcatcaggca ccgggccggt
     2341 gaccgatgac ggacgggtcc aaggaaagcc cgatgccccc ttgaccttga tcgaatattc
     2401 ggactttacc tgtggctatt gtttgaagtt ttttcaggaa actttaccgc ggttgcaggc
     2461 ggcgtatctt gataccggga aagtcaaatt cgtgtaccgc gactatccgc gatccgatcg
     2521 cggtgtcggc gtcgaggccg cggtggcagc ccgttgcgcc ggagcgcagg ggcgctattg
     2581 ggccatgcat gatcggttgt tcggtgaagg cagacggttg gattccgggt cttttaagag
     2641 ggtagccaaa tccgtgggat tggacgagat gcagtttgga aagtgttttg atgagcggcg
     2701 gtacttggaa tcaatttttc aagaccgtca agaggcgaat cgttggggat ttcacggtac
     2761 acccggattt attttgatgc agacggtcgc tggcccgaca gaaaaggtcc ctgctgtcgc
     2821 catacccggg gcttttccgt ttgaggcctt tgccgaggaa atcgatcgta tgctgtccaa
     2881 ggtacaggga tcgtagtcgt cacagcgcag ccgagaccga gcgagaatta cccatcacgt
     2941 tatccttaca cgaaggatta ccgtatgtct gatacacagt ctttctggtc cgtccctcag
     3001 cgcgatggtg aacctccctt ctgggtgtgc atgtcctgtc tttcagaagc gttctatcgc
     3061 aaagtcccga tgccggattg ccctaattgt cacggggtgt ccacctacga agcatttgcg
     3121 atcgaagcca tcgccgattg ggggaccgag gatttggtcg ccaaggccaa ggcttctgaa
     3181 caagcggcga acgtcgaacc tgcgtccgca tcctcagtag aatcctcgac gtaagccgat
     3241 cgtccaagcg gagtgtcttt ttgcaaggac cacgtccatt ccttgttggc ggccactggc
     3301 gccaaagtga cacacgcgct ccggtgcacg atcccttcac cggcaaggtc ctggctgagg
     3361 tttcgcaagg cagcgaggcc gacgctgagg atgccatcca atcaacggcc gctgcggctc
     3421 aaccgatggg cgcgctgcct tcgcacgcca gatttcacct tttacaacgc attgctggag
     3481 cgctctacga tcgacgggaa gaatttgcca ggatcatgac tggcgaagcg ggaaagccca
     3541 ttacggatgc acgtcgggaa gtcgcgcgtg ccgtccaaac cttcaccatc gcagcggaag
     3601 aggccaagcg aataccaggc gaggtgattc cactcgactg gacgcccggt atggactctc
     3661 atcttggcat tcttcgtcgg gttccgatcg ggccagtgct cggaatcacg cccttcaact
     3721 ttccgttgaa tcttgtcgcc cataaggttg cgcctgcctt ggccgccggc aatccgatat
     3781 tgatcaagcc ggcgccccaa actccgttga ctgccctgct gttggctgag gtgggactcg
     3841 aggctggagt tccccccggc ggcctcaacg tgctgccttg tgacaaccgt gttgcagagc
     3901 gcctggtcgt ggatcctcgc ttcaagctct taagtttcac cggcagtgcg gcggtcggat
     3961 ggatgttgaa ggcaaagtgc ggaaaaaaga aagtcgtact ggaactggga ggcaatgccg
     4021 gcgtcatcgt ggaacaggat gccgacctgg actttgcggc tcaacgctgt gcgagcggag
     4081 gattcggcta tgcggggcaa acctgcattt ccgtccagcg catttttgtg caccattctg
     4141 tggttgatct cttcacgacg aagttgctgc tccaggtggc ccgtttgaag ggcggcgatc
     4201 ccaacgacga ggcgaccgtc gtgggcccgt tgatcgatca acacgcggcg catcgcgtcg
     4261 aagaatggat cggagaggca gtcgcccaag gtgcacgggt gctgttaggc ggcaaacgca
     4321 tgggttccgt cgtggaagcc accgtgctca ccaatgtcac gccgacgatg aaggtctcct
     4381 gtcaggaagt gtttggtcca gtcgtcaccg tgacgcccta tcggcagttc gacgaggctc
     4441 ttgaggcccg gacccgttcc gagtatgggc tgcaggccgg agtttttacg caagacgtca
     4501 acaaggcatt tcatgcgttc cgtcatttgg aagtgggagc cgtgcttgtc aacgagattc
     4561 cgacgttccg ggccgagcat atgccttacg ggggcgtgaa ggactccggc ctcggccggg
     4621 aggggttgcg gatctcgatc gaagagatga ccgaaccccg attcctcgtc ttaaatgtcc
     4681 ggccgccgtc gagtgcataa gcgaaagaag catcagcata ttgcgatccg tggacattct
     4741 tgatacaaca gcgaccctgt gcctagaaaa ctcgcgccag ccttgacgtt tgtggccgac
     4801 tgcaatgccg accggacgaa gcaagggaga aggagacgat ggtacctacg cacatgtttc
     4861 tgactagagg agtcggcgtc caccgggaaa agctggcgtc ctttgagcaa gcgcttcgca
     4921 gcgccggcgt cgcctactgc aa
//
LOCUS       DASSCQ010000137        15802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1690412, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000137 DASSCQ010000000
VERSION     DASSCQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15802
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690412"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VFH05_09355"
     CDS             complement(<1..984)
                     /locus_tag="VFH05_09355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6678463.1"
                     /translation="MIKAMKDFQIPMRVEVDKETASATFGRFTTEAYERGFGTTVGNA
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTSIILNIKGLRLASHTDKPKTI
                     RLKKKGPGEAKGSDIIHDADIAILTPNLHIATLDKDATLDMEMTVKHGRGYVPAERNK
                     EEGLPIGVIAIDSIFSPIKRVNFHVENARVGRMTDYDKLTLEIWTDGTISPSDALSTA
                     AGILRDHVDIFINPEARVEGRAELSGDDAQREINKNLFRSVNELELSVRAANCLKNAN
                     IKTIADLVQKSEAEMLKTKNFGKKSLNEIKEILTDMGLSLGVKLDAMASGSG"
     gene            complement(1063..1689)
                     /gene="rpsD"
                     /locus_tag="VFH05_09360"
     CDS             complement(1063..1689)
                     /gene="rpsD"
                     /locus_tag="VFH05_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET6678464.1"
                     /translation="MAKYRGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QARPRISDYSTQLREKQKLRRIYGLQERQFRGVFERAERQSGVTGDALLRLLECRLDN
                     VAYRLGFGASRKEARQLVGHGHVTVNGRKNNIPGAQVRVGDVVAVRERSRTLVSIQTA
                     LESVEGRGIPEWLDLDKAGFKGTVRALPTKDQITLPVNEQMVVELYSR"
     gene            complement(1767..2150)
                     /gene="rpsK"
                     /locus_tag="VFH05_09365"
     CDS             complement(1767..2150)
                     /gene="rpsK"
                     /locus_tag="VFH05_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET6678465.1"
                     /translation="MSVKKGKKKERRIVQSGIAHVQASFNNTIVTITDMSGNTVVWAS
                     AGNQGFKGSRKSTPFAAQRAGEAAARKAMESGMRQVDVYVNGPGSGRESAIRSLQGAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(2169..2564)
                     /gene="rpsM"
                     /locus_tag="VFH05_09370"
     CDS             complement(2169..2564)
                     /gene="rpsM"
                     /locus_tag="VFH05_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET6678466.1"
                     /translation="MARIAGVDLPREKRSDIGLTYVFGIGRAAARNILDKAGIDGAIR
                     VKDLSEDQIVKIREIIDQEYQVEGDLRKTFSMNIKRLIDTGTYRGLRHRKGLPVRGQR
                     SKTNARTRKGRRAGVGSKPKPAARPAPTA"
     gene            complement(2877..2990)
                     /gene="rpmJ"
                     /locus_tag="VFH05_09375"
     CDS             complement(2877..2990)
                     /gene="rpmJ"
                     /locus_tag="VFH05_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET6678467.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRILCINPRHKQRQG"
     gene            complement(3004..3222)
                     /gene="infA"
                     /locus_tag="VFH05_09380"
     CDS             complement(3004..3222)
                     /gene="infA"
                     /locus_tag="VFH05_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET6678468.1"
                     /translation="MPKEDIIEIQGSVLETLPNAMFRVQLDNGHKILAHISGKMRMHF
                     IRILPGDKVTVEMSPYDLSRGRITYRFK"
     gene            complement(3263..4012)
                     /gene="map"
                     /locus_tag="VFH05_09385"
     CDS             complement(3263..4012)
                     /gene="map"
                     /locus_tag="VFH05_09385"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6678469.1"
                     /translation="MIILKTPDEIALMAKASRVVAEALEVLKSATKPGVTTDELDRLA
                     EAEIRSRGALPAFKGYRNYPKTLCVSVNEQVVHGIPSKRVLKDGDIVGLDLGAIVGGF
                     YGDSAVTIAVGRIDEKAATLVRVTEESLSLAIQQARVGNRLSDISHAVQQHVEAAGYS
                     VVTEFVGHGIGRQLHEEPQVPNYGKPGQGPRLQPGMVLAIEPMVNMGGSAVRVLDDRW
                     TAVTVDGSLSAHFEHTIAIQPSGPALVLSRL"
     gene            complement(4009..4665)
                     /locus_tag="VFH05_09390"
     CDS             complement(4009..4665)
                     /locus_tag="VFH05_09390"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET6678470.1"
                     /translation="MRVVFLGAPGVGKGTQADRVAAQFKIAKISTGDLLREAVRIQTP
                     LGREAKQYMDQGRLVPDSVVIGLVRDKLAEAPCANGFVLDGFPRTVPQAEELEQVLRA
                     TQRPLNRVVTFKVPREVVIKRLSGRRSCPKCQATFHVEFAPSQKGALCDRCGETLVQR
                     SDDKPEAIETRLKVYEEQTAPLIQYYANKRLLSELDASGSVDAVFEALFGVLAPYSAL
                     "
     gene            complement(4673..5992)
                     /gene="secY"
                     /locus_tag="VFH05_09395"
     CDS             complement(4673..5992)
                     /gene="secY"
                     /locus_tag="VFH05_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET6678471.1"
                     /translation="MFERLLTSFQNILKIPELRTRILFTLGMLVVYRVGAHIPTPGIN
                     GEALSEFLQKQGGALLGFLDIFSGGSLSRLTIFALGIMPYISASIILQLLTVVVPHLS
                     KLAKEGERGRKKIIQYTRFGTIGIALVQGFGIAIGLEQMNGGAFVLDKGWAFRLMTVI
                     TLTAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPSAVAQTYNLYEIGQLNAFLL
                     VGLAALMVAVVAAIVFLESGRRKVPVQYAKRVVGRRVYGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWIKAIGAQLSPGSLLYTLMYVGLILFFCFFYTAVVLNPV
                     DMADNMKKYGGFIPGIRPGQRTSDYIYKVLTHITFAGALYLAIVCVIPEFLIYKLNVP
                     FYFGGTSLLIVIGVGLDTAQQIESHMLMRNYEGFLGKGMGPLRGRSG"
     gene            complement(5985..6434)
                     /gene="rplO"
                     /locus_tag="VFH05_09400"
     CDS             complement(5985..6434)
                     /gene="rplO"
                     /locus_tag="VFH05_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET6678472.1"
                     /translation="MKLHDLAPAKGAKKKRKRIGRGPGSGHGKTATKGHKGLLARSGG
                     GKQPGFEGGQMPLIRRVPKYGFTNPFRKEYAIVNIERLADLPASGTITPQAMVDAGLV
                     KRVTLPIKILGNGELKKAISVQAHKFSKSAEAKIQAAGGSVEVIPGV"
     gene            complement(6431..6637)
                     /gene="rpmD"
                     /locus_tag="VFH05_09405"
     CDS             complement(6431..6637)
                     /gene="rpmD"
                     /locus_tag="VFH05_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET6678473.1"
                     /translation="MATAKPPKTAAHVRITLKKSPIGTPERHRLVLRGLGLRKIRQSV
                     VRPDTPQVRGLIHQVGYLLEVQPS"
     gene            complement(6637..7140)
                     /gene="rpsE"
                     /locus_tag="VFH05_09410"
     CDS             complement(6637..7140)
                     /gene="rpsE"
                     /locus_tag="VFH05_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET6678474.1"
                     /translation="MRVNPDELSLKDKVVFINRVAKVVKGGKRFNFCALVVVGDGQGW
                     VGIGKGKAAEVPVAIAKAVQQAKKNLVHVPLKSGTIPHEVHGLFGGEHVLLKPAVEGT
                     GIIAGGAVRAVVELVGAHNIISKTLGRGNPFNTVRATLDGLTQLRHPDEVLRLRRGEE
                     LQTGALA"
     gene            complement(7162..7527)
                     /gene="rplR"
                     /locus_tag="VFH05_09415"
     CDS             complement(7162..7527)
                     /gene="rplR"
                     /locus_tag="VFH05_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET6678475.1"
                     /translation="MNSAAKLRHLARRQQRVRQRVFGTDERPRLNVFRSSGHIYAQII
                     NDLKGTTLAAASSLDKSLRTSLKSTSSIAAAKAVGTLLAERAKSAKVQKVVFDRGGRL
                     YHGRIKALADASREGGLQF"
     gene            complement(7536..8072)
                     /gene="rplF"
                     /locus_tag="VFH05_09420"
     CDS             complement(7536..8072)
                     /gene="rplF"
                     /locus_tag="VFH05_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET6678476.1"
                     /translation="MSRIGKKPIPLPTGVDVKVAGSVVSVKGPLGKLDWTLKPGIGVT
                     VDKGQVVVTRSSDERKVRAMHGLVRAELNNMVEGVTKGYERNMEITGVGYKAAIQGRT
                     MSFNVGYINPVLYPVPTGIDVKVDKQTLINIKGSDKRLVGQVAANLRAIKPPDVYKQK
                     GVRYVGEVLRKKEGKTGK"
     gene            complement(8116..8511)
                     /gene="rpsH"
                     /locus_tag="VFH05_09425"
     CDS             complement(8116..8511)
                     /gene="rpsH"
                     /locus_tag="VFH05_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET6678477.1"
                     /translation="MVTDPISDLLVRLRNGFRRHHDVVTVPASKLKREVLRVLQAEGY
                     IQGIDSGTEDGHPVLKVQLRYVGEGQPMITGMQRVSKPGRRVYVGTKDIPRVRNGIGM
                     AILSTSKGIMTDQASRKAGLGGEVLCSIW"
     gene            complement(8580..8765)
                     /locus_tag="VFH05_09430"
     CDS             complement(8580..8765)
                     /locus_tag="VFH05_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET6678478.1"
                     /translation="MSRLALRNKTATKPKFATRGYHRCGLCGRVHGYLRRFKMCRICF
                     RLLSLKGEIPGVRKSSW"
     gene            complement(8786..9445)
                     /gene="rplE"
                     /locus_tag="VFH05_09435"
     CDS             complement(8786..9445)
                     /gene="rplE"
                     /locus_tag="VFH05_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET6678479.1"
                     /translation="MAEAKDPKKSKGAKPSGRPAKKDSGKAPQVLDDGSQESKFQPRL
                     RDTYRDRVVPALMKEFNYKNVMQVPKLERIVINVGMSEAIQNVKLLESAATELGTITG
                     QKAVVTRAKKAIAGFKLRQGMPIGTKVTLRSRRMYEFFDRLVTLALPRIRDFRGVSPK
                     AFDGRGNYTLGLKEQLIFPEIQYDEVGSIHGMDITIVTTARTNDEGKALLTQLGMPFR
                     T"
     gene            complement(9552..9872)
                     /gene="rplX"
                     /locus_tag="VFH05_09440"
     CDS             complement(9552..9872)
                     /gene="rplX"
                     /locus_tag="VFH05_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET6678480.1"
                     /translation="MQKAHIRKGDTVVVIAGRDRGKSGKVLSVDRSAGKVIVEKLNII
                     KRHTKPNQKVKQGGILEKEAPLAISNVMYLCPVTKKPTRLGIRVQPDGRRVRFSKKSN
                     ELVE"
     gene            complement(9900..10268)
                     /gene="rplN"
                     /locus_tag="VFH05_09445"
     CDS             complement(9900..10268)
                     /gene="rplN"
                     /locus_tag="VFH05_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET6678481.1"
                     /translation="MIQNYTYMDVADNSGAKQAMCFHVLGGSKRRYASLGDVIVVAVK
                     EAIPQATVKKGDVSRAVIVRTTKEVRREDGSYIKFDRNACVLINKEGEPIGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            complement(10305..10571)
                     /gene="rpsQ"
                     /locus_tag="VFH05_09450"
     CDS             complement(10305..10571)
                     /gene="rpsQ"
                     /locus_tag="VFH05_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET6678482.1"
                     /translation="MAETGVKRREWVGRVISNKMNKTVVVAVERQVVHPIYHKVLRRL
                     TKFKAHDEQNSCNIGDRVRMIETRPISKDKHWRVVEILEKGRVD"
     gene            complement(10592..10810)
                     /gene="rpmC"
                     /locus_tag="VFH05_09455"
     CDS             complement(10592..10810)
                     /gene="rpmC"
                     /locus_tag="VFH05_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET6678483.1"
                     /translation="MSTMDVKELRQLSSDELAEKAKQLAQELFNLRFQMGTGRLENPM
                     QIRKTKRSIAQVKTVLREVQTADHKKAS"
     gene            complement(10828..11241)
                     /gene="rplP"
                     /locus_tag="VFH05_09460"
     CDS             complement(10828..11241)
                     /gene="rplP"
                     /locus_tag="VFH05_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET6678484.1"
                     /translation="MLAPKKVKFRKMMKGRMRGKAYRGGHITLGEFGLKALEPGWVTS
                     RQIEAARIAITRYVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     LYEMGGVTPDIAYEAFRLASHKLPIATKCVTRGEF"
     gene            complement(11256..11927)
                     /gene="rpsC"
                     /locus_tag="VFH05_09465"
     CDS             complement(11256..11927)
                     /gene="rpsC"
                     /locus_tag="VFH05_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET6678485.1"
                     /translation="MGQKTHPIGYRLGYNYTWSSRWYASKDYAKLLHQDIKIRKVVKQ
                     KLFHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKAELEKQYGGQVYITV
                     KEIKKPELDAQLVSENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMIAGRLGGAE
                     IARTEWYREGRVPLHTLRAEVDYGFAEAHTTMGQIGVKTWIYKGEILPAQPLKPESAF
                     ERRLG"
     gene            complement(11979..12338)
                     /gene="rplV"
                     /locus_tag="VFH05_09470"
     CDS             complement(11979..12338)
                     /gene="rplV"
                     /locus_tag="VFH05_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET6678486.1"
                     /translation="MAEARAVLRFVRVAPRKARPVVDMIRGQQVPMALAMLKHLPRHA
                     ARVIEKVLRSAVANAEQKELGDSDSMTVSKAFVNCGPIYKRFRARSMGRANAIQKRTS
                     HITVVVEARGMQEKSRS"
     gene            complement(12388..12675)
                     /gene="rpsS"
                     /locus_tag="VFH05_09475"
     CDS             complement(12388..12675)
                     /gene="rpsS"
                     /locus_tag="VFH05_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET6678487.1"
                     /translation="MPRSISKGAFVDDHLLKKVEQMNQTKDRKIIKTWSRRSTVIPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGQAKTEKAVALK"
     gene            complement(12668..13510)
                     /gene="rplB"
                     /locus_tag="VFH05_09480"
     CDS             complement(12668..13510)
                     /gene="rplB"
                     /locus_tag="VFH05_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET6678488.1"
                     /translation="MGLKAYKPTTSGRRGMTIVTTEELTKKRPEKSLTSFLLRTGGRN
                     NDGRTTVRFRGAGHRRLYRTIDFRRDKVGISAKVSALEYDPNRSARIALLTYRDGEKR
                     YILAPIGLQVGDVLQSGVDAEVRTGNALPLSNMPLGTTIHNIELKPGKGGQLIRSAGG
                     FAQVMGRDGNYVQVRLKSGEMRRVLATCMATVGQVGNLDHENINVGKAGRSRWLGRRP
                     HVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGLPTKGYKTRQNKKTDKFIIARRKSG
                     VRNA"
     gene            complement(13658..13951)
                     /locus_tag="VFH05_09485"
     CDS             complement(13658..13951)
                     /locus_tag="VFH05_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET6678489.1"
                     /translation="MKLDLHSVLIQPLLTEKVTALRESSNTVGFLVHPDANRLQIKQA
                     VETLLKVKVARVNVLNVQGKIKRLGRFSGKRSDWKKAFVKLKEGEKLELYESA"
     gene            complement(13948..14571)
                     /gene="rplD"
                     /locus_tag="VFH05_09490"
     CDS             complement(13948..14571)
                     /gene="rplD"
                     /locus_tag="VFH05_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET6678490.1"
                     /translation="MPTVDVLDLNKRKVGSAELPPEVFGCKPHATLVHEAVVMQRACE
                     RQGTASTLRRGEVRGSGKKPWKQKHTGRARAGSVRSPVWRHGGSVFGPKPRSYAFNMP
                     KKKYRTALQSALSSKVTDGQLFVVSSLSLEQPKTKVLAKALQLFAAGAQVLVIAGEGQ
                     DGLLQAARNLSKVQVVTPDRLNVYDIVRAGTIVIPERALARVKEVWA"
     gene            complement(14574..15194)
                     /gene="rplC"
                     /locus_tag="VFH05_09495"
     CDS             complement(14574..15194)
                     /gene="rplC"
                     /locus_tag="VFH05_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET6678491.1"
                     /translation="MTNGLLGKKLGMTQMLDETRLTPVTVIEAGPCRVVMVKTKERDG
                     YEAVQLAFGQVKDRQLSKAQLGHLKKHEATASRILREFKKDGDVAVGQTVKADIFKKG
                     DWVDVIGVSKGKGFQGVVRRHHYAGGPESHGSMFHRAPGSIGASSFPSRVWKGKTLPG
                     HMGAERVTSQRLKVIESRPDENLLFVRGAVPGAANGLVVVRKSKKG"
     gene            complement(15257..15571)
                     /gene="rpsJ"
                     /locus_tag="VFH05_09500"
     CDS             complement(15257..15571)
                     /gene="rpsJ"
                     /locus_tag="VFH05_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET6678492.1"
                     /translation="MVKVDQRIRIRLRGFDYRVLDQSVAEIVETVRRSGAKIVGPIPL
                     PTRIEKFTVQRATHADKKAREQFEIRTHKRLMDIMEPTPETMDSLMKLNLAAGVDVEI
                     KL"
     gene            complement(15595..>15802)
                     /gene="tuf"
                     /locus_tag="VFH05_09505"
     CDS             complement(15595..>15802)
                     /gene="tuf"
                     /locus_tag="VFH05_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET6678493.1"
                     /translation="YRPQFYFRTTDVTGVVSLNPGVEMVMPGDNVSVTAELISPIAMD
                     QGLRFAVREGGKTVGSGVVTEILA"
BASE COUNT         3015 a         5035 c         3998 g         3754 t
ORIGIN      
        1 accgctgcct gaggccattg catcgagctt gaccccgagg ctcaatccca tatccgtcaa
       61 aatttctttg atttcattca gcgacttctt gccaaaattc ttggtcttca gcatctccgc
      121 ttctgacttc tgcacgagat ccgcaatggt cttgatgttg gcgttcttta agcagttcgc
      181 tgcgcgcacg gacaactcca gttcattgac gcttcgaaag agatttttat tgatctcccg
      241 ctgggcgtcg tcgccgctca actcagcgcg gccttccacc ctggcttccg gattgatgaa
      301 aatatccaca tggtcacgta agatccccgc ggcggtcgac aaggcatcgc tgggcgagat
      361 ggtcccgtcg gtccagatct cgagggtcag tttgtcatag tcggtcatcc ggccgacacg
      421 ggcattttca acgtgaaagt tcacgcgctt gatcggagaa aaaatggaat caatggcgat
      481 gacgccgatc ggcaagcctt cttccttgtt gcgttccgct ggtacatagc cgcgcccatg
      541 cttgacggtc atttccatgt cgagcgtggc atccttgtcg agtgtcgcga tgtgaagatt
      601 gggtgtcagg atggcgatgt cggcatcgtg aatgatatcc gaccccttgg cttcgccagg
      661 gcctttcttt ttcaaccgga tcgttttcgg cttgtccgtg tgggaggcca accgtaatcc
      721 cttgatgttc agaatgatcg acgtgacgtc ttccgtcaca ccaggaatcg tcgaaaactc
      781 gtgcagcacg ccttcgatct tcacggtcgt caccgccgct ccggtgaggg atgagagcag
      841 gacacggcgc agggcattgc cgacggtcgt gccgaagcca cgctcgtagg cttccgtagt
      901 gaatctccca aaggtggcgg aagcggtttc cttgtccact tcaacccgca tcgggatttg
      961 aaaatctttc atcgctttaa tcatgagtcc cccttcatcc tgtcagccgc cgcactttct
     1021 gcgatgcact ctgcacaaag ccggcgttgt atgaagttgc cgttaccgcg aatacaattc
     1081 gacgaccatc tgttcgttaa ccgggagcgt gatctggtcc ttggttggta acgcccgcac
     1141 ggttcccttg aagccggcct tatcgagatc caaccattcc ggaatgcctc gtccttcgac
     1201 cgactctaac gccgtctgga tcgataccag ggtccggctt cgttcccgca ccgccaccac
     1261 gtcgccgaca cggacttgcg ccccggggat gttgttcttc ctgccattga ccgtcacgtg
     1321 cccatgaccg accagttggc gggcttcctt gcgtgaggcc ccgaacccca ggcggtaggc
     1381 cacattatcg agacgacatt cgagcagcct cagcaaggcg tcgcccgtca cacccgattg
     1441 tcgttccgcc cgttcaaaca caccccggaa ctgcctttct tgaagtccat atatccgtcg
     1501 cagtttctgt ttctctcgca actgagtgct gtaatctgag atcctcggcc gggcctggcc
     1561 atgctggccg ggtggatagc tccggcgctc aatggcacat ttttcggtca tgcaacgcga
     1621 gccttttaag aacaacttct cgccttcacg acgacacagc cgacagactg gaccgcgata
     1681 ctttgccact tgctctacct ccggttttcg ggcacgccct tcatggtctg cccgcttggt
     1741 tccgattcac ctctgcgcat tttcttttag actcgccgtc gcttcggagg cctgcagccg
     1801 ttatgcggga ttggcgtcac gtcacggatt agattaatcc gtagtcccgc cccctgtaac
     1861 gaacgaatcg ccgactcgcg tcccgatccg ggaccattga catacacgtc cacctgacgc
     1921 atgccgcttt ccatcgcctt tcttgcggca gcctccccgg ccctctgtgc ggcaaacggc
     1981 gtgcttttac gcgatccttt gaagccttgg ttgccggcgc tcgcccacac caccgtattg
     2041 ccgctcatgt ctgtgatggt cacgatggtg ttattgaacg aggcctggac atgagcgatg
     2101 ccgctttgga cgatccgtcg ttctttcttc ttgccttttt ttacgctcat gacagtctcc
     2161 tacaatgact atgcggtcgg cgctggacgg gcggccggtt ttggcttgct gccaactccg
     2221 gcacgacgcc cttttcgcgt gcgcgcattg gtttttgaac gctggccgcg gacgggcaac
     2281 cctttacgat gacgcaggcc ccgatatgtt cccgtatcga tcagacgctt gatgttcatc
     2341 gagaatgttt ttcggagatc gccctcgacc tggtattcct gatcgataat ctcgcggatc
     2401 ttgacgatct ggtcctcact gagatctttg acccggatgg cgccatcgat cccggccttg
     2461 tccaaaatgt ttctggcggc cgcccggccg atgccgaata cataggtcag gccgatgtcc
     2521 gaccgttttt ctctcggcaa atccactccc gcaatacgtg ccatatctcc tcctaccttc
     2581 ccttcaaggt cttccgcagc cgaggctgga tgagcctctt ctcacccgcc cacccctcgg
     2641 cgccaagacg ccgccttccg gtggcgcgct tgaacgagtt ctccaggcta gcttgcttct
     2701 tctcataagg agcagcctgg atcagtccaa aggtgcgcgc gtgaaggagc accgcttacc
     2761 taaaaatacg cttggtctgg tgtgccgcct tccgcgagca caaggactga ccaggttgct
     2821 ccatcccatt ctggtgtccg cgttcggcga ggctcgccag cctccggatg ccccaactat
     2881 ccctgccgtt gtttgtgccg aggattgata cagagtattc ggaccacccc acggcgtctc
     2941 acgactttac acttggcaca aatcggcttg accgatgatt tcactttcat ggacgacaga
     3001 ctcctacttg aagcgatacg taatacgccc acggctcaga tcatacggcg acatttccac
     3061 cgtgaccttg tccccgggaa gaatgcggat gaagtgcatg cgcattttcc cggaaatgtg
     3121 cgcgagaatc ttatggccgt tgtcgagttg cacgcgaaac atggcgttgg gcagcgtctc
     3181 cagtaccgag ccttggatct cgatgatgtc ttccttcggc acgaaccgtt accctttctc
     3241 ccggtgaggc acgcctgctg tgctataacc gactcaggac gagtgcggga ccactcggct
     3301 gaatcgcaat cgtatgttca aagtgagccg agaggctgcc gtcgacggtt accgccgtcc
     3361 agcgatcgtc caacacccgt acagccgatc cccccatgtt caccatcggt tcaatcgcca
     3421 acaccatgcc cggctggagc cgaggaccct gccccggctt cccgtagttc ggcacctgcg
     3481 gctcctcgtg tagctggcgc ccgatcccgt gtccgacaaa ctcggtgacg accgaatagc
     3541 cggccgcttc gacatgctgc tgaaccgcat gagaaatatc ggacagccgg ttcccaaccc
     3601 gcgcctgctg aatcgccagt gacagcgatt cttccgtcac tcgaaccagc gtggctgcct
     3661 tctcatcaat tcgcccgaca gctatggtca ccgccgaatc gccatagaat ccgccgacaa
     3721 tcgcgcccaa atccaacccg acaatatcgc cgtccttcag cacccgcttg gagggaatgc
     3781 cgtgtacgac ctgctcattg acggacacgc aaagcgtctt cggatagttc cggtaccctt
     3841 tgaacgccgg aagcgcgcct ctgctgcgaa tttcggcttc cgccaaccgg tccaattcat
     3901 ccgtcgtcac gccaggcttg gtggcgcttt tcagcacttc cagcgcctct gccaccactc
     3961 ttgacgcctt tgccatcagg gcaatctcgt ctggcgtctt gaggatgatc atagcgcgct
     4021 gtagggcgcc aggacgccga acagggcttc aaacaccgca tcaacggaac cggaggcatc
     4081 cagttccgag aggagccgct tgttggcgta atactggatt aacggcgccg tttgttcctc
     4141 gtacaccttc aaccgtgttt cgatcgcttc cggcttgtcg tcgctccgct gaaccaacgt
     4201 ctcgccgcac cgatcacaca aagcaccctt ctgagacggg gcgaactcca catggaaggt
     4261 cgcctgacac ttcgggcaac tgcggcgccc gctcaaccgc ttgatgacca cctcacgcgg
     4321 gaccttgaag gtcacgaccc tattgagagg ccgttgggtc gcccgcagga cctgttccaa
     4381 ttcttcagcc tgtggaacgg tacgcggaaa gccgtccagt acaaatccgt ttgcgcaagg
     4441 agcctcggca agcttgtctc gaacgaggcc tatgacgacc gaatcaggga ccagcctacc
     4501 ctggtccatg tactgctttg cctcacgccc taacggggtc tgtatgcgaa ccgcctcacg
     4561 caacaagtcg cccgtcgaga tcttggcaat cttgaattga gccgcgaccc gatccgcctg
     4621 cgtccctttg ccaactcccg gcgcaccgag aaacacgacg cgcatgcggt cctcacccgc
     4681 tccggccgcg gagtggaccc attcccttac ccaagaatcc ttcgtagttg cgcatcaaca
     4741 tgtgcgattc gatctgctga gccgtatcca agccgacgcc aatgacgatc aacaacgacg
     4801 tcccgccgaa gtaaaacggg acgttcagct tgtaaatcag aaattcgggg atcacacaga
     4861 cgatggccag atacaatgcc ccggcaaatg tgatgtgcgt cagtacctta tagatataat
     4921 cggatgtcct ctggcccggc cggatgccgg gaatgaaccc tccgtatttc ttcatgttgt
     4981 cagccatgtc gaccgggttc aggaccactg ccgtgtaaaa aaaacagaaa aacagaatca
     5041 gaccgacata catcagtgta tagagcaaag atcccggcga cagttgagca ccgatcgcct
     5101 tgatccacgg cgtttcaaaa aatcccgcga tggtcgccgg aaacgcaatg atcgacgaag
     5161 caaaaatcgg cgggatcacg cccgccgtat tgattttcaa cggaatatga gtgctctggc
     5221 cgccatagac tcggcgaccc accacccgtt tcgcgtattg caccggaact ttgcgccgtc
     5281 cactctccaa aaacacaatt gcggccacga cggccaccat cagcgccgca aggccgacca
     5341 gcaggaatgc gttgagttgg ccgatctcat acagattata ggtctgcgcc acggccgaag
     5401 gcagacgtgc cacgatgccg gcaaagatga tcagggagat cccgttgccg atgccgcgtt
     5461 ccgtaatctg ctcgcccagc cacatcaaga acccggtacc ggccgtcagt gtaatcacgg
     5521 tcatcagccg gaacgcccag cccttgtcta agacaaacgc gccgccgttc atctgttcga
     5581 ggccgatggc gatcccgaat ccctgaacca atgcgattcc gatggtgcca aaacgcgtgt
     5641 actgaatgat cttcttgcga ccccgttccc cctccttagc cagcttcgaa agatggggaa
     5701 ccaccaccgt caacagctgc aagatgatcg atgcgctgat atagggcatg atgcccagcg
     5761 caaagatcgt cagcctcgac agtgaccccc ctgaaaaaat atcgaggaac ccaagaagcg
     5821 cccctccctg tttctgcagg aactcagaga gcgcttcgcc gttgatacca ggtgtcggga
     5881 tatgtgcgcc gacccgatac accaccagca tccccagcgt aaacaggatg cgtgtccgca
     5941 gctcgggaat ttttagaata ttctgaaagc tggtcaggag ccgctcaaac accggggata
     6001 acctcgactg accctcccgc tgcttgaatc ttcgcctcag ccgacttgct gaacttgtgc
     6061 gcctgcacgg aaatggcttt cttcaactcg ccattgccca gaatcttgat cggcaatgtg
     6121 acccgcttaa ccaggcccgc atcgaccatc gcctgcggcg taatggtccc cgaagctggg
     6181 agatctgcca gacgctcaat attgacgatt gcgtattctt tccggaaggg attggtgaag
     6241 ccgtacttcg ggacgcgacg aatgagaggc atctggccgc cttcgaaccc cggctgcttg
     6301 ccgcctccgg agcgagccag caatccctta tgccctttcg ttgccgtctt cccatgaccc
     6361 gagccggggc cacgtccaat acgcttccgt tttttcttgg cccctttagc cggcgcgaga
     6421 tcgtgtagtt tcatgatggc tgcacttcca gaagatagcc cacttggtga atcaatcccc
     6481 gtacctgcgg cgtatccggg cggacaaccg actggcggat cttgcgtaag cccagcccgc
     6541 gtaggacgag gcggtgccgc tcaggtgtgc cgatgggact tttcttcaac gtgatgcgca
     6601 catgagccgc cgtcttgggt ggttttgccg ttgccattac gccagagctc ccgtctgaag
     6661 ttcctcgccc cgacgcaatc gcaagacctc atcggggtgc ctgagctgtg tcaaaccgtc
     6721 gagggtcgcg cgtaccgtgt tgaacgggtt tcctcggccc aacgttttgg aaatgatgtt
     6781 gtgcgctccc accaactcaa cgaccgctcg gaccgctccg ccggcgataa tgccggttcc
     6841 ctcgaccgca ggcttcaata agacatgttc accgccgaac aacccgtgca cttcgtgagg
     6901 aatagtcccc gatttcagcg ggacatggac gagatttttc ttcgcttgct ggaccgcctt
     6961 tgcaatggcg acgggcacct ccgcggcttt gcctttgccg atgccgaccc atccctgtcc
     7021 gtcaccgacc accacgaggg cgcaaaaatt gaaccgcttg ccgcccttca caaccttcgc
     7081 aacacggttg atgaacacga ctttgtcttt gaggcttagt tcgtctggat tcactcgcac
     7141 gctcgttgct cccttcgtag cctagaattg caacccgcct tcacgggagg catcggcgag
     7201 ggccttgatc cgcccgtgat acaacctccc cccccgatca aagaccactt tttgaacctt
     7261 ggcacttttc gcgcgttctg ccaacaatgt acccacggct ttggccgcgg caatggagct
     7321 ggtcgacttc aaactcgttc gcagggattt atccagcgag gaggcggcag ccagcgtcgt
     7381 gcccttcagg tcgttgatga tttgtgcata gatgtgcccg ctgctgcgaa acacattgag
     7441 ccggggacgc tcgtcggttc cgaagacccg ctgacgaacc cgctgctggc gacgtgcaag
     7501 atgacgtaat ttagctgcgc tgttcatggt tcaacctatt ttcccgtctt gccttctttc
     7561 tttctcaaca cttcgcctac gtaacggact cccttttgct tgtagacatc aggtggcttg
     7621 atcgctcgca gattggccgc gacttgcccc accagccgct tgtccgaacc tttgatattg
     7681 atcagcgttt gcttgtcgac cttgacgtcg atgccggtcg gcaccggata caacaccgga
     7741 ttgatgtatc ccacattgaa gctcatggtc cgtccctgaa tggctgcctt ataaccgacg
     7801 ccggttattt ccatgttgcg ctcatagcct ttggtcacac cctcgaccat attattcaac
     7861 tcagcgcgaa ccaggccgtg catggcgcgt acttttcgct cgtcgctgga tcgcgtgacg
     7921 acgacctgtc ccttgtcgac ggtgacaccg attccgggct ttaacgtcca gtcgagcttc
     7981 ccaagcggcc ccttcacgga gaccacggat cctgccacct tcacatcgac gccggtcgga
     8041 agagggatcg gtttttttcc aatgcgtgac atgccttcct catctttcct gctgaaaaac
     8101 cccttatcaa aatccctacc aaatcgaaca cagcacttcg ccgccgaggc cggctttgcg
     8161 cgaggcttgg tcggtcatga tacccttcga tgtggaaaga atcgccatgc cgattccgtt
     8221 tcgcacacgg ggaatgtcct tcgtccccac atacacacga cgtcccggtt tgctgacgcg
     8281 ttgcatgccg gtgatcatgg gctgcccttc cccgacgtag cgcaattgga ctttcagaac
     8341 aggatgcccg tcttcggtcc cggagtcgat gccctgaatg tagccctcgg cttgcagcac
     8401 gcggagcact tcccgcttca attttgaagc gggaaccgtc acgacgtcat gatggcgccg
     8461 gaagccattg cgcagtctca ccaataaatc gctgatagga tcggtcacca tgcgaatccc
     8521 tttactccct cttcattcac cttcattgaa caacgcttcc cgcctgtcca agctgattgt
     8581 caccaactgg acttccgaac tcccggaatc tcgcctttca gactcaggag gcgaaagcaa
     8641 atccggcaca ttttgaatcg ccgcaaatac ccgtggacgc gcccgcacaa accgcatcgg
     8701 tggtagccac gtgtcgcgaa tttcggtttg gtcgcggtct tatttctgag cgctaatctc
     8761 gacacgctga ctccctccct tttggttacg ttcggaacgg catgcccaac tgggtgagca
     8821 gggctttgcc ttcatcgttc gtccgagccg tggtcacgat cgtgatgtcc atgccgtgaa
     8881 tcgaaccgac ctcgtcgtac tggatctccg gaaaaatgag ctgttccttc agacccaacg
     8941 tgtaattgcc ccggccatcg aacgcttttg gagacacgcc ccggaaatcg cggatgcgcg
     9001 gcagcgccag cgtcacgagc cggtcgaaaa actcgtacat gcgccggctc cgcaacgtca
     9061 ccttggtccc gatcggcatt ccctgccgca gcttgaatcc cgcaatcgct ttcttcgcgc
     9121 gggtcaccac ggccttttgt cccgtgatcg tacccaactc cgtcgcagcg ctttccagca
     9181 gcttcacgtt ttggatggct tcgctcatgc ccacgttgat cacgatgcgt tcgagcttgg
     9241 gcacctgcat gacgttctta tagttaaact ccttcatcaa tgccggcacc acccggtcgc
     9301 gatacgtgtc gcgcaaccgc ggctggaact tcgattcctg gctgccgtcg tccaacactt
     9361 ggggagcctt gccgctgtcc tttttcgccg gccggcctga cggctttgcg cccttgcttt
     9421 ttttcggatc ctttgcctcg gccatacaac cttcttttct ggcagcctgg tgaaaagttc
     9481 ctccagcttg tcctcgccct tactctcggc gcttcgctga aaggcctttg tgcctgctgc
     9541 tccctctctg actactcgac taattcgttg gactttttgc tgaaccgcac ccggcgtccg
     9601 tccggctgca cacgaatgcc caagcgcgta ggctttttcg tcaccgggca gagatacatc
     9661 acgttggaaa tggcgagcgg ggcttctttt tccagaatgc cgccctgctt caccttttga
     9721 ttcggcttgg tatgccgctt gatgatgttc agcttttcta cgatgacctt ccctgcggaa
     9781 cgatcgacgg agagaacctt gccggacttt ccacgatcgc ggccggcgat gaccaccacc
     9841 gtatcccctt tacgaatatg agccttctgc agcgcgtgca cgattacccc tacagttggt
     9901 taaagcactt ccggagccag tgagatgatt ttcataaatt ttttccagcg aagctcccgt
     9961 gcgaccggtc cgaaaatgcg ggtcccgatg ggctcaccct ctttgttaat gagcacacag
    10021 gcattccgat cgaatttaat ataggagccg tcttcgcgcc ggacttcttt cgtcgtccgc
    10081 acgatcactg cccggctgac gtcgcctttt ttcaccgtcg cctgtggaat ggcttccttg
    10141 acggccacaa caatgacatc tcccagtgag gcgtagcgcc gcttcgaccc gcccagtacg
    10201 tgaaagcaca tggcctgctt cgcaccggaa ttatcggcta cgtccatgta tgtgtaattc
    10261 tgaatcatga tgcgtttctc gcctcgtcgg gcacgtcccc gttactagtc gacacgcccc
    10321 ttttccaaaa tttcgacgac ccgccagtgc ttgtctttgc tgatgggtct ggtctcgatc
    10381 atccggacgc ggtctccgat gttgcaagag ttctgctcgt catgggcctt gaatttcgtc
    10441 aaacgccgga gcaccttgtg ataaatggga tgcacgactt gacgctcgac tgccacaacg
    10501 acggtcttgt tcatcttgtt gctaatgacg cgccccaccc attctcgccg cttgactcca
    10561 gtttccgcca tgctctgcaa ccctttcgcc gctaggacgc cttcttgtga tcggccgttt
    10621 gaacttcgcg cagcaccgtt tttacttgtg cgatcgaccg cttggtctta cgaatctgca
    10681 tgggattctc gagccgaccg gtgcccattt ggaaccggag attgaatagt tcctgggcca
    10741 attgcttggc cttctcggca agctcgtcgc tgctcaactg ccgcaactct ttcacatcca
    10801 tcgtgctcat ggtttcatgt cccagtctta aaactcaccc cgggtcacgc atttcgtggc
    10861 gatcggaagc ttgtgtgaag ccaggcggaa cgcctcatac gcgatatctg gagtgacacc
    10921 acccatttca taaaggatcc ggccaggctt gaccacggct acccaatatt ccggattgcc
    10981 cttgcctttt cccatgcgtg tttcggccgg ttttttcgtg atcggcttat cggggaaaat
    11041 tctcgtccac acctggccgc cgcgtttcac gtaacgcgtg atggcaatcc gcgcggcctc
    11101 gatctggcgg ctagtgaccc atcccggctc caatgctttc aacccgaact cgccgagcgt
    11161 gatgtggccg ccacggtagg ccttgccccg catccgccct ttcatcattt ttctgaattt
    11221 cactttcttc ggtgctagca caatgccctc gtttcctacc caagccgccg ttcaaatgcg
    11281 gattccggct tgagcggttg agccggaagg atttcaccct tgtaaatcca ggtcttgact
    11341 ccgatctgtc ccatcgtcgt gtgcgcttcg gcgaagccat agtcgacttc cgctcggagc
    11401 gtgtgcaacg gtacccggcc ctcgcgatac cattctgtgc gcgcaatttc agcgccaccg
    11461 agacggccgg caatcatgat cttgatgcct tgtgcaccaa ggcgcagtgc ggattggacg
    11521 ctgcgtttca tggctcgacg gaaggccacc cgtttttcca actgcgttgc cacgttttcg
    11581 ctgaccagct gcgcgtccag ctccggcttc ttgatttctt tcaccgtgat gtagacctgt
    11641 ccgccgtact gcttttccaa ttccgctttc agcttgtcca cctcggcgcc tttgcgtccg
    11701 atgatgattc ccggtcttgc cgtgtggata atgacgcggg tttgatcgcc ggaccgctcg
    11761 atctccacct tcgccacacc cgcatgaaac agcttctgct tcactacttt gcggatcttg
    11821 atgtcctgat ggagcagctt tgcatagtcc ttactcgcgt accagcgtga actccatgtg
    11881 tagttgtagc cgagccgata accgattgga tgtgttttct gacccatgcg atccgcacct
    11941 cagttctgcg gcggcacgtc tgccgatccg ctcaatgttc aggaccttga cttctcctgc
    12001 atgccccgcg cttcaaccac cacagtgatg tggctcgttc gcttctgaat ggcattcgcc
    12061 cgtcccatcg atctggcacg aaagcgcttg tagatggggc cgcagttcac gaatgctttc
    12121 gacaccgtca tcgaatcact gtcacccaat tccttctgtt ctgcattggc caccgccgac
    12181 cgcagcacct tctcgatcac acgtgccgcg tggcggggaa gatgcttcaa catggccagc
    12241 gccatgggaa cttgttgccc gcgaatcata tccaccaccg gacgggcctt gcgtggtgca
    12301 acccgaacaa atctgagcac ggctcgcgct tctgccatct ttcccatcct gtcatgcgtc
    12361 tcttccgacg cacgacaact gatctcccta tttcaatgcc acggcttttt cggttttggc
    12421 ctgcccgtgc cccttgaaga acctcgttgg tgcaaattcg cccaacttat ggccgaccat
    12481 gttctcagtc acaaagaccg gaataaattt tttgccgttg tgtacggcaa acgtatgccc
    12541 gatcatgtcg ggaatgacgg tggatcgccg tgaccaggtc ttgatgattt tgcggtcttt
    12601 ggtttgattc atctgctcga cttttttgag caggtgatcg tcgacaaatg ctcccttgct
    12661 aatcgatcta ggcattgcgc acccctgact tccggcgagc gatgatgaac ttgtccgtct
    12721 ttttattctg tcttgtcttg tagcctttcg tcggaagacc ccaaggagag acaggatgcg
    12781 gattgccctg acccgacttg ccttcaccgc ctccgtgtgg atggtcgacc gggttcatga
    12841 caaccccgcg aacatgcgga cggcgaccga gccaccggct ccgccccgct tttcccacat
    12901 tgatgttctc atggtccagg tttcctacct ggccaaccgt ggccatacag gttgccaaga
    12961 cgcgtcgcat ttctccggac ttgagccgca cctgcacata gtttccgtca cggcccatga
    13021 cctgagcgaa tcctccggcg ctgcggatca actgaccgcc tttgccgggc ttcaactcga
    13081 tattatgaat cgtcgtcccc aacggcatat tggaaagagg cagcgcattc ccggtcctga
    13141 cttccgcatc cacccccgat tgcagcacgt ccccgacttg caggccgatc ggtgcgagga
    13201 tgtagcgttt ctctccatct cgatacgtga gaagcgcgat tctggccgag cggttgggat
    13261 catactcgag cgccgaaacc ttggcggaga ttcccacctt gtcacgccgg aaatcgatgg
    13321 tcctgtacaa gcggcgatga ccggccccac gaaaacggac cgtcgtacgt ccatcgttat
    13381 ttcggccgcc ggtcctgagg agaaatgacg tcaacgactt ctcgggccgc tttttcgtga
    13441 gttcttccgt cgtcacgatc gtcatgcctc gccggcctga cgtcgttggc ttataggctt
    13501 tcagtcccat atcagcttcc tattcattcc acctgagggt gttgcagtgt ctagtcccaa
    13561 ctgcgcgcgt ccaccgagca ccgccctttt cgataaccat tcgatacaac cttgtgtgcg
    13621 cggtgcgcga gcacagggac tagccctgcg acgcccccta cgcactttcg tacaattcga
    13681 gcttctcgcc ttccttcaac ttcacgaagg ccttcttcca atcagagcgc ttgcctgaga
    13741 accggcccag gcgcttgatc ttgccttgca cgttcagcac gttgacgcgc gcaactttca
    13801 ccttcaagag cgtctcaacc gcctgcttga tctgtagccg attggcgtcg ggatgaacga
    13861 gaaaacccac cgtattgctg ctttcccgca gtgcagtcac tttctccgtc agcaacggtt
    13921 gaatcagtac actgtggaga tcgagtttca tgcccagacc tccttgaccc gagccaaggc
    13981 tcgttccggt atcacgatcg tgccggcacg cacgatgtca tagacattca gccgatcagg
    14041 cgtcacgacc tggaccttcg acagatttcg ggcggcctga agcagtccat cctgcccttc
    14101 gccggcaatg accaggacct gggcgccggc ggcgaagagc tgcagcgctt tagccaacac
    14161 cttggtctta ggctgctcga gcgagaggct ggacacgacg aacagctgcc cgtctgtgac
    14221 cttcgacgac aatgcgcttt gcagcgccgt gcgatatttc tttttcggca tgttgaacgc
    14281 gtagcttctc ggcttgggcc cgaaaacgga gccgccgtga cgccatacgg gcgatcgcac
    14341 cgacccggca cgggcccgac cggtgtgctt ctgcttccag ggttttttgc cggatcccct
    14401 gacttcaccc cgccggagcg ttgatgcagt gccctggcgt tcgcaggccc gctgcatgac
    14461 caccgcttcg tggacgagcg tggcatgcgg cttacatcca aacacttcgg gcggcaattc
    14521 agcggacccg actttccgtt tgttcaaatc aagcacatct accgtcggca tgcttatccc
    14581 tttttcgact ttctcacgac cacgagtccg ttcgctgcgc cgggaaccgc cccgcggaca
    14641 aacaacagat tctcgtcagg cctcgattca atgactttca atcgttggga ggtgacgcgt
    14701 tcagcgccca tatgtccggg cagggttttt cccttccaga cccgtgatgg aaacgagctt
    14761 gccccgatcg atcccggcgc ccgatgaaac attgatccgt gcgattccgg tccaccggca
    14821 tagtgatgcc gccgtaccac cccctgaaat cccttcccct ttgacacgcc gatgacatcc
    14881 acccagtcgc cctttttaaa aatgtcggcc tttacggttt gcccgacggc tacatctccg
    14941 tctttcttga attctcttaa tatccgactg gccgtcgcct cgtgcttttt caaatggcct
    15001 aactgcgcct tggagagctg gcgatccttc acctgtccga aggccaattg aacggcttcg
    15061 tagccatccc gttccttcgt tttgaccatg actacgcggc acggtcccgc ctcgatgacg
    15121 gtcaccggcg tcagccgggt ctcatcgagc atctgcgtca tgccgagttt tttgccaagt
    15181 aacccgttag tcataaatga tccgcttcgc agtggtgagt gccgcactca gtgtggtcgg
    15241 ctggggctga gcccactcag agtttaattt caacatcgac gccggcagcg agattcagct
    15301 tcatgaggga gtccatcgtt tccggagtcg gctccatgat atccatcaaa cgcttgtgcg
    15361 ttcggatttc aaattgctca cgcgcctttt tatccgcatg cgtcgcccgc tgaaccgtaa
    15421 atttctcaat gcgtgtcggc agcggaattg gacccacgat tttcgcaccg ctgcggcgaa
    15481 ccgtttcgac aatttccgca acggattgat ccaacacgcg ataatcgaac ccccgcagcc
    15541 taattctgat tcgctgatcg accttgacca tgcaatcccc tgttcgtccg tttcctaggc
    15601 cagaatttcc gtgacgactc cggatccgac cgtcttgccg ccctcgcgca cggcaaagcg
    15661 taacccctgg tccatcgcga tcgggctgat caattcagcc gtcacgctca cgttgtcgcc
    15721 cggcatcacc atctccaccc ctggattcaa cgacaccacg cccgtcacgt cggtggtccg
    15781 gaaatagaac tgcggccgat ac
//
LOCUS       DASSCQ010000138         8677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_563988, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000138 DASSCQ010000000
VERSION     DASSCQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563988"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFH05_09510"
     CDS             complement(<1..233)
                     /locus_tag="VFH05_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET6678494.1"
                     /translation="MILPISPFAILVLGLVLMLMGFNVLREYERAVIFRWGRLARGLV
                     GGNGPGVVIIIPFIDKLVRVSLRTVTMDVPPQDV"
     gene            complement(230..1558)
                     /locus_tag="VFH05_09515"
     CDS             complement(230..1558)
                     /locus_tag="VFH05_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET6678495.1"
                     /translation="MPVQGRRPLQSLHLSFSLVIALFSLPATSAAEPFVTLATYDGVI
                     NPVSAEYLHDALATAEASGAQALIFSLNTPGGLDSSMRLIIKDISGATIPVIVYVSPS
                     SARAASAGVFITMAAHVAAMAPGTSIGAAHPVNMGGGEMDKTMKEKVENDSVAYIKSI
                     AQQRERNAAWAEDAVRKSVSVTEREALKLKVVDLIAEGLPALLQQLDGRRVVLASGPT
                     VLKTAGARVQEFPMGVRLEWLKAISDPNIAYLLMTVGTIGIIAELYSPGAILPGIVGA
                     ISLILAFYSFQSLPVNYAGVLLFLLGIVFFVLEASVTSYGLLAIGGVLSMLIGSVMLI
                     KSDAEFFRISWSVILPVVTMASAFSLLSIGMGLKAMRRRPLTGREEMVGLVGIVKTAL
                     APQGQLAVHGELWEAVSEQPLSPGDEAEVTGVDGLRLRVKPYLRKKEVHT"
     gene            complement(1689..2708)
                     /locus_tag="VFH05_09520"
     CDS             complement(1689..2708)
                     /locus_tag="VFH05_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6678496.1"
                     /translation="MDVLSEVLKAVKLDGAVFYTAECSAPWGFRSPPSRVMASYLSPE
                     PKHVIVYHLVTEGRGYAHIEDDTKPVPLEAGEIVVFPHGHPHILGNGAPIVPVDSAKE
                     LQRILSEDLKIYRHGGGGELTRIICGYLACEPQLSKILLSGLPPILKVSIRNDASGEW
                     LENSLRYSVDNSDASRPGGETVLAKLSEVLFIETLRRYIEQLPQKQAGWLAGVRDPEI
                     GKALAFLHRQPARPWTIAALAAEVGMSRSVLAERFRQYLSETPMAYLARWRLQLGAQM
                     LHATSRSVAEIAGEVGYESEPSFNRAFKREFGLPPARFRSHSRAARRTSPAPAMANSR
                     PQMTR"
     gene            2903..3724
                     /locus_tag="VFH05_09525"
     CDS             2903..3724
                     /locus_tag="VFH05_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6678497.1"
                     /translation="MATSILTPEVETLKTRLRTMWMAGDYDRFSRYMEGSAREFYDRI
                     PRFPGCTFLDVGCGSGQLALIAARDGLHVTGVDIAANLIERARSRAQAENLQARFEEA
                     DAEALPFEDESFDVVGSLIGAMFAPQPEAVAGELVRVCKPGGTIAMANWTPQGFVGHM
                     FKAVSKFIAPSGMPSPVLWGDEATVRERFGQGLTELRLTRRHYTFMYDFSPAAVVELF
                     KLYYGPINQAFGALDAAGRDSLRRELEELWAEHNRSGLDRTTVYAEYLEVIGIRA"
     gene            complement(5039..5428)
                     /locus_tag="VFH05_09530"
     CDS             complement(5039..5428)
                     /locus_tag="VFH05_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6678498.1"
                     /translation="MPVQYRVLLVDDDPGTRQLLQDILEKYKDIRIVGQAGDGKQAVA
                     MAVEHQPDVILMDIGLPYLDGVEATQCIRKSCPETVVICLTGHFSPPRYSAMRTAGAA
                     AFVCKNQVLAIHEMILSTLGQWTSWKY"
     gene            complement(5846..6238)
                     /locus_tag="VFH05_09535"
     CDS             complement(5846..6238)
                     /locus_tag="VFH05_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6678499.1"
                     /translation="MRRLYDHIDLRVHSLQEAREFYEAVLPALGFTRRMEVDGWLQFE
                     AVGAAGESTEFFGVTEASGHTANECRIAFWAQSTAEVDRLAHIGAEKGGRQIEGPGYL
                     EGAGYYAVFFEDPSGNRLEICHRVAGAI"
     gene            6374..7282
                     /locus_tag="VFH05_09540"
     CDS             6374..7282
                     /locus_tag="VFH05_09540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6678500.1"
                     /translation="MFRLIQHRASSMPTRVLGKTGVNLPILGFGTAHSGLRLNTREAV
                     HLYERAFGEGITYFDTAPEFAGYGKAQMQLSYFLKQVRPQVFLVTKCYEPDGERALQL
                     LQKSLKELGTSYADLVFVHSVGADKMDPRIVFGNKGTYAALMKAKALGLTRYVGLSGH
                     SRPGRFAQAIRQFDVDVLLNAVNFVDQHTYNFEAEVWPAAARANIGLIAMKVYGGAQD
                     PPPPPDRLSHSMMAAEYHDLAFRYALSLPQVCCAVIGMATTRELEQNVARARAFTPLS
                     GSEVRRLRDAGGLMARQWNDHFGPVT"
     gene            complement(7356..8054)
                     /locus_tag="VFH05_09545"
     CDS             complement(7356..8054)
                     /locus_tag="VFH05_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678501.1"
                     /translation="MHPRFNGSSTKGPEGLRRIGSAFLFGLILAAAVTGCSNAEFVPD
                     ITLAPARPIIDATAEFHDLHPAPALLSGKESFGLAGPDAKAVPPRVLANQVQEELLDA
                     FEQAGVFSKITPFDRQPDLILTGRINALHEHYRPRVWTKLPLPYFGKMAAGMLDLKTH
                     SSSGEADLTLFLLTTNGELIGTYRGSSSFDENFNPTGEMAPGARLNRALADAVQKIQD
                     QMLHDSRLRTIASR"
     gene            complement(8023..8337)
                     /locus_tag="VFH05_09550"
     CDS             complement(8023..8337)
                     /locus_tag="VFH05_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678502.1"
                     /translation="MKTVLYSMITAVIAMNMTLAGCAKNASERRSASPTMSERISKDS
                     VKGRVSEIGAKYVSIRDTHGESRRVRVDDNTKMDQVAVGDQVKAYVTEDGYASTIQRV
                     VD"
BASE COUNT         1896 a         2438 c         2538 g         1805 t
ORIGIN      
        1 acgtcctgcg gcgggacatc catggtgacg gtccgcaaac tgacgcggac cagcttgtcg
       61 atgaacggga tgataatgac gacgcctgga ccattcccgc ccacgagccc gcgggccaga
      121 cggccccagc ggaagatgac ggcccgttcg tactctcgca gcacgttaaa gcccatgagc
      181 atgagcacga ggccaaggac gagaatcgca aaaggactga tggggagaat catgtgtgga
      241 cctctttctt cctcaggtaa ggtttgacgc gtagtcgcaa gccgtcgacg cctgtgactt
      301 ctgcttcgtc gccaggcgac aagggttgct cgctgaccgc ttcccagagt tctccgtgga
      361 cagccaactg cccctgcggc gcgagcgccg tcttgacgat accgacgagg ccgaccattt
      421 cttcgcgccc ggtcagaggc cgccggcgca tggccttcaa gcccatcccg atgctgagca
      481 gggaaaacgc cgaggccatc gtgacgaccg gcagaatgac cgaccacgaa atccggaaga
      541 actcggcatc gctcttgatc agcatcaccg atccaatcag catcgacaac acgccgccga
      601 ttgccagcaa gccgtaactg gtcacactgg cttccaacac aaaaaagacg atgccgagca
      661 ggaagagcag gaccccggcg taattgaccg ggagtgattg gaaggaatag aacgcgagga
      721 tgaggctgat cgcgccgacg atgccgggaa ggatcgcccc cgggctgtag agttctgcga
      781 tgatgccgat tgtaccgacc gtcatgagca ggtaagcgat gttgggatcg ctgatcgctt
      841 taagccattc gagacggacg cccatgggaa attcctggac cctggctccg gctgttttca
      901 gcaccgtcgg acctgacgcc agaacgacgc gacggccgtc caattgctgg agtaaggccg
      961 gcagtccttc tgcgatgaga tccacgactt tgagcttcaa ggcctcgcgc tctgtgacgg
     1021 acacgctttt gcgcaccgca tcctccgccc aggctgcatt gcgctcccgc tgttgagcaa
     1081 tcgacttaat gtaggcgacc gaatcgttct ccaccttttc cttcatcgtc ttgtccatct
     1141 caccgccgcc catgttcacg ggatgggcgg ctccgatgct cgtgccggga gccattgcgg
     1201 ctacatgggc ggccatggtg atgaacacgc cggcagaggc ggcacgtgcg ctggatggag
     1261 acacgtagac gatgaccggt atggtcgccc cggaaatatc tttgatgatc aggcgcatcg
     1321 aactgtccaa tccacccggc gtattgagcg aaaaaatcag tgcctgggcg ccgctcgctt
     1381 cggccgtggc caacgcatca tgcaggtact ctgctgaaac cggattgatc acgccgtcgt
     1441 aggtggcgag ggtcacaaaa ggctccgcgg cgctggtggc cgggagagaa aagagggcaa
     1501 tcacgaggct gaatgaaagg tgaagggact gcagcggacg gcgaccctgt accggcatga
     1561 acgcgtccct cccaccaagc aagggtcttg gagacggaac aaatcgacag gagcatacga
     1621 gccgaatttt tcactgtcaa ggaaggggaa tccagcgggt tggtctgagg gcgagcagca
     1681 ggccgcttct atcgtgtcat ctgtggtcga ctgttcgcca tggcaggggc gggggaggtg
     1741 cggcgggctg ctcgcgagtg gctacggaat ctggccggcg gcagcccaaa ctcccgtttg
     1801 aaggcacgat taaaggacgg ttccgactcg taccccacct caccagcgat ctctgcaaca
     1861 ctgcggctcg tggcatggag catttgcgcg ccgagctgaa ggcgccagcg ggcgaggtag
     1921 gccatcggcg tttcggatag atattgccga aagcgctcgg ccagtaccga acgggacatc
     1981 ccaacctcgg ccgcgagtgc ggcgattgtc catggacggg caggttgacg atgcaggaac
     2041 gccaaggctt tcccgatttc cggatcccga acacccgcca gccagccggc ctgtttctgt
     2101 ggaagctgct cgatgtagcg tcgcagggtt tcgataaaca acacctccga caacttcgcc
     2161 agcacggtct caccaccggg tctggacgca tcggaattgt caaccgaata gcgaagagag
     2221 ttttccaacc actcgccgga ggcgtcattc cgtatactga ccttcaggat gggcggcagt
     2281 ccgctgagaa gaatcttgct cagctgcggc tcgcaggcca aatatccgca aatgattctc
     2341 gttaattctc cgcctccgcc gtgccggtaa atcttaaggt cctcagacag aatgcgctgc
     2401 agttctttgg cgctgtcgac aggcacgatg ggcgcaccgt tgccgaggat gtgcggatga
     2461 ccgtgtggaa agaccacgat ttctcctgcc tcgagaggaa ccggctttgt gtcgtcttcg
     2521 atgtgtgcat agccgcgccc ctcggtcacc aaatgataga cgattacgtg tttaggttcc
     2581 ggagacagat aagaagccat gacgcgtgag ggaggcgaac gaaagcccca cggtgcagaa
     2641 cattcagcgg tgtagaagac agccccgtcg agtttgacgg ccttgaggac ttcggacaat
     2701 acatccacgg aatgtgcctc tcagcgcgtc cggcaaacga aaccggacgg tcggtcaagt
     2761 gatgcgcagc ctagcacgcc cgcgtcaaga gaaccagact ctcggataag cctaccccca
     2821 atggatcgcc tatagtgacg acacatcaaa ggagcagcgg aggacggtgc ttcgattgga
     2881 tcacccaaaa caggaggtca acatggcgac cagtatcctc acaccggagg tggagacatt
     2941 aaagacgcgc ctgagaacaa tgtggatggc gggggactac gatcgattct ctcgctatat
     3001 ggaaggcagc gcgagggaat tctacgaccg catccctcgt ttcccgggct gtacgtttct
     3061 ggatgtcggc tgtggctcgg gtcagctggc gttaatagcg gcacgggatg gattgcacgt
     3121 gaccggcgtc gacatcgcgg ccaatctcat cgaacgcgcg aggagccggg cccaagccga
     3181 aaatctgcag gctcgattcg aagaagcgga tgcggaggcc ctgcccttcg aggacgaaag
     3241 tttcgacgtc gtgggaagcc tcatcggggc gatgttcgca ccgcagccgg aggcggtcgc
     3301 cggggaactg gtgcgtgttt gcaagccggg tggaacgatt gcaatggcca attggactcc
     3361 gcaggggttc gtgggacaca tgttcaaagc cgtgtcgaaa ttcattgccc catccggcat
     3421 gccctccccg gtgttgtggg gtgacgaagc cacggtgcga gaacggtttg gacaggggct
     3481 cacggaactc cgcttgacca ggcgtcacta taccttcatg tatgattttt cgcctgcggc
     3541 cgtggtggag ctgtttaaac tgtactatgg accaatcaat caagcattcg gtgcgcttga
     3601 cgctgcgggg cgcgacagcc ttcgcagaga actcgaagag ctgtgggctg agcacaaccg
     3661 ttcagggtta gatcgcacga ccgtctatgc ggagtatttg gaagtcatcg gcattcgggc
     3721 atagctgcgg tggagacaac accacgggat ccagacgggg cggtaggcac gacatgaagc
     3781 attcgagaaa tttgatcaag cggaaacccc tgtcccggaa gcctgccatg acagaggttg
     3841 ccgggctatc cgacagccag gtttgcggtc gcgcgacaaa agcagtgatc aatcagcggc
     3901 cgacttagga gaatccctag taggggggtg acgatatcag accgtatcat tttaaagagg
     3961 ctgatgttcg ataacccaag gagggacgag ttatgaagac atggacattc atagcgttct
     4021 tgatcgccgg cgtatgcctt attgctgcag atcatgcgct ggctcaagca ggatccggag
     4081 gttcgggttc tggtggatca agcggaagcg gcatgacctc tcagggtctt gcgtctgaac
     4141 cggccggtat aggtgagcga ggaactgggc aaacccaaag cacgctcgag cgatccggag
     4201 ctcctgcaca gaaaggaatg tcctctgaac ccgcagggat tggcgagaag gggacgggac
     4261 agacacaaag tacgactgag cggtctgggc ttggacaaag cagtgctcaa agtcaatctc
     4321 cacttccttc cggtgtgggc gaacagggta cagggcagac ccagagcaca ctggagcggt
     4381 ccggtggtgg acaaggtgcg actcaaggcc aaacctctca aagcgcaatg gaccgtcccg
     4441 aacgttgacg gtcgaattcc cttccctctc atggaggcgc cggacttccg gcgcctccat
     4501 gcgcaacttc tccgcgcaac ttctctacat ctctcttgag ttcaacgcgt tcctcatatc
     4561 ccagtgatct cggcatgact gcgttcccca ccgctcgaag accagggaat ttccttgctt
     4621 gacacgtttg ctgagtcggc gtacaggtga gattgctgac tcccgatatt ccttccgaac
     4681 gatggaggag gtcatcatgc ccgacctgaa tccaccggac catcaccgcc ctcccagtca
     4741 tggctgggct gctcgtcggt catgctagca ggtcttcatc agccgccgcc gtcgtcccgc
     4801 gaagggacta tcagatagac tttttagggc gcagtgacga agacgggact agcaacgcct
     4861 ggaatgtcag ttgactgtcc gctgacgagt tgccctgttt atcatggcga ggagggtctg
     4921 tgttgcagac atagcaatcg atctcggcgt cataagcgcc acaaacgggc gaccttcgca
     4981 tgtctgcgtg gtcgcccgtg ttgtatgatc agatgtaagg cagacgcgag gtgtcagtct
     5041 agtacttcca gctcgtccat tgtccgagcg tcgacaagat catctcatgg atggcaagca
     5101 cctggttctt acacacgaat gcggcggcgc cggcggttct catggcgctg tatcggggag
     5161 gggaaaagtg gccggtgagg cagatcacga cggtctccgg acatgatttc ctgatgcact
     5221 gggtcgcttc aactccgtcg agatagggga gcccgatgtc catcaagatc acatcgggtt
     5281 ggtgttcgac agccattgcc acagcctgct tgccgtctcc ggcctggcct acgatcctaa
     5341 tgtctttgta tttttcaagg atgtcttgaa gaagctggcg cgtccctgga tcatcgtcga
     5401 cgaggagcac acggtattgg actggcatgg cgtcaatcga agatgccgac actgaaggac
     5461 ctcgggaagc caagccattg tggaagccat gattgccgtc tactgcgaac ataacgacct
     5521 ctctgtacga ataacgatca catcgggcat gacagaagca cacggtgctg tcacgtttct
     5581 gtatctttct ctcttataaa ggggtcgatc atgaacgcgg cctagtaata acccaagtct
     5641 cgaccggttt cttccttcat gcagcacgcg tctagtattg gggtggatca caacgtgaat
     5701 tgcgggaccc atcatatctt gggaaaatct ccgactacca atggtctatg agcattggta
     5761 gtactaagtg agcaggccta ggaagtgtta aaaactcagg ttggtgttca catcggcgtt
     5821 gggaacctga agagcgagta tcgggtcaaa tggccccggc tactctatga cagatctcga
     5881 gtctgttacc gctcggatcc tcaaaaaaca ccgcataata gccagctccc tccaaatagc
     5941 ctggcccttc aatctgacga cctcctttct cagcaccaat gtgagctaat cggtcgacct
     6001 cggctgtact ctgtgcccaa aatgcaattc gacactcatt cgcggtgtgt ccactggcct
     6061 ccgtgacgcc aaaaaattcc gtcgattcac ctgctgctcc aaccgcttca aattgcaacc
     6121 agccgtcgac ctccatccgg cgggtgaatc ccaacgcggg caacacagcc tcataaaact
     6181 cgcgggcctc ctgcaggcta tgcacccgaa gatcaatgtg atcgtaaagc cttcgcatca
     6241 attttcattc ataggggaag cgctattcta ctggtccgct gttcggcggt ctatcgtcta
     6301 ggttccttcc cagtcactga gacgctggcg agtcagttat gcttgcactc cattatggac
     6361 attgtcacct agtatgtttc gcttgataca acaccgggcc tcatccatgc cgacgcgcgt
     6421 gctcggaaag accggtgtga acttgcccat cctggggttc ggcaccgcac atagcggcct
     6481 tcgcttgaac accagagagg cggtccatct ctatgaacga gccttcggcg aaggcattac
     6541 ctattttgat accgcgccag agtttgcagg ctacgggaag gctcaaatgc agttgagtta
     6601 ttttctcaag caagtgcgtc cccaagtgtt tcttgtcacg aagtgctatg agccggacgg
     6661 tgagcgggcc ctgcagctcc tgcaaaagag cctgaaggaa ctcggcacca gttatgccga
     6721 cttggtgttc gttcacagcg tcggtgccga caaaatggat cctcggatcg tgtttgggaa
     6781 caagggtacc tacgctgcat tgatgaaggc caaggcgctc gggctgacac ggtacgtcgg
     6841 tttatcagga cacagccgcc cggggcgctt cgcgcaagcg atacgccaat tcgacgtcga
     6901 tgtgttgttg aacgccgtca atttcgtcga ccagcatacc tacaattttg aagcagaggt
     6961 gtggccggcg gcggcccggg ctaacattgg tctcatcgcg atgaaggtgt atggcggcgc
     7021 tcaggatccg ccaccgcctc cggaccggct cagtcattcg atgatggcgg ccgaatatca
     7081 cgatcttgcc tttcggtatg ccttgagtct gccacaggtc tgctgcgccg taatcggcat
     7141 ggccacgacg cgtgagctcg agcagaatgt tgccagggca cgggcattta cgccgctatc
     7201 gggcagtgag gtgagacgct tgcgagatgc cggcgggctg atggcccgcc aatggaacga
     7261 tcatttcgga ccggtcacgt aactgcgagg agatcacggc gagtgactct gtgatgcgtt
     7321 ctgccggtgg tctttagctg gctccaactc tgcagtcagc gtgaggcgat tgttctcaga
     7381 cgggagtcat gcagcatctg atcctgtatt ttttgcaccg catcagcgag cgcgcggttg
     7441 agccgcgcgc ctggggccat ttctccggtc ggattgaaat tctcatcgaa tgaggaagaa
     7501 ccacgatacg tgccgatcag ttcaccgttt gtggttaaga gaaaaagcgt gagatcggcc
     7561 tccccgctgc ttgagtgcgt tttcaagtcc agcatgccag ccgccatttt gccgaaatag
     7621 ggcaaaggaa gtttggtcca cacccttggg cgatagtgct cgtgtaaggc gttgatccgg
     7681 ccggtgagaa tcaggtccgg ctgccggtcg aatggggtga ttttcgaaaa tacccctgct
     7741 tgctcgaatg catccagcag ctcctcttgc acctgattcg ccagcacgcg aggcgggact
     7801 gctttcgcat caggccctgc gaggccaaag ctctccttgc ccgacagcag cgccggggcc
     7861 ggatgcaggt cgtggaattc cgcggtggcg tcaatgatcg gacgtgccgg cgcgagggta
     7921 atatccggca caaattcagc gttgctgcag cctgttacgg cagccgccag aatcaatcca
     7981 aagaggaaag cggatcctat ccggcgcaat ccctcaggac ctttagtcga cgacccgttg
     8041 aatcgtggat gcataaccat cttccgtgac gtaggctttc acctgatcac cgactgccac
     8101 ttggtccatt ttcgtgttgt catcgacgcg tacacgcctc gactctccat gagtatccct
     8161 gatcgagacg tacttggctc cgatctcact cacgcggcct ttcactgaat ctttagagat
     8221 gcgctcgctc atcgtcggcg atgcggaccg gcgctcggac gcattcttgg cgcaccccgc
     8281 gagcgtcatg ttcatcgcga tgacggctgt aatcatcgag tagagaactg tcttcatagg
     8341 acacctcctt tgcgttccct tctgaatgta cctgtcggac ctggctggcc acgctaggaa
     8401 cgccactagg tacaggcctc ctcaacccaa gcactgaagt aatgtagggt ttttacttct
     8461 ctgactgaag gggtacgtag ctagcgaaac ctctagcctt cggaaaaccc cttgctttgg
     8521 gccatcttgc gcaggccggc taagcggcat gtggcgtgca atccttcact gccagctagc
     8581 ttccaatgcc ttgacgatag atgaagagtg aattgaataa gagacacaag aaaggaagat
     8641 cgacatgaga atcgcacaag tctcgcctct gtgggaa
//
LOCUS       DASSCQ010000139         8064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_449628, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000139 DASSCQ010000000
VERSION     DASSCQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449628"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1165..1335
                     /locus_tag="VFH05_09555"
     CDS             1165..1335
                     /locus_tag="VFH05_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678503.1"
                     /translation="MLSTITLVVPCVEKESVFLQLAIQAAIQAHRDHPKSYEVKEGTV
                     NNDRSGEFSLPL"
     gene            1916..3544
                     /locus_tag="VFH05_09560"
     CDS             1916..3544
                     /locus_tag="VFH05_09560"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HET6678504.1"
                     /translation="MSITTTSLPEGEDNEPYSASLSGSGGTLPYRWFVSPALPANLSL
                     DTATGAITGTPTTQATTTHTFTLRDSSVPSETVQQTLRLTIRPVPAFLGIITASLPNG
                     TVNQAYNRPVQASGGTPPLTWNIVAGTGTLPPGLNLNSTTGAISGTPTAAGTSSFTVR
                     VTDARGESDTERLSITIGAGPAPPNPPNITTTTLPAGTVGQPYSQPVQVTGGTGALTW
                     SISAGTLPAGLELNQINGAISGTPTAAGTSPFTVRVDSGGQFDMQALSITINEPAPPP
                     SPSITTTTLPAGTVGQTYNQTLGATGGTGGLTWSIVAGNLPAGLNLDQPSGVISGTPI
                     VPAGASSFTVRVADAAGQDDTQALSITINLPAPPNITTTMLPGGTVGQPYAQTLQATG
                     GIGTLTWSFTGSLPAMLSFSPGGVISGTPTNTGTSNFTVTVRDTLNLSDTQPLSITIS
                     AANPAPDLVTLSVNTSGPGEGIVTSNPGGISCPSTCVASFPRGTIVTLTATANQGSTF
                     DEWRGGPCNNRSGTCQLTMNDNQSANARFEDEDD"
     gene            4910..6232
                     /locus_tag="VFH05_09565"
     CDS             4910..6232
                     /locus_tag="VFH05_09565"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HET6678505.1"
                     /translation="MFTALYRTAAVLLTLFLSLSGCSSGGDTQSASTTTTGTSPAAND
                     PSTPATPNTGPETGGSESTGSDGTIPVHQLADLNGDVTDLALHPDGDIVVAGTFSTYG
                     GQPVPHVIKLRPDGSLAPWTLGVSLISSLPIETAISPDGKVWIAQRQGEVARLWRVSD
                     DGTLDSGFVPVDFLFDETGGGVIPSQVFQLEPIGNGVYVVGTFTRVNGQPRRGLVRIS
                     ATGQVDGGFNADIASSAYRLAPNPDGTLYVTNFERLDVSVVGSQILYRLNADGSRVSG
                     FSPVNVYTVGFNARIYALARVGDDTNDVLIGGGFVLDYSGTVNPLLDPTAIHNLARVN
                     PDGSRDRVQPRPQLGTDSTNTPIVRAIAKTIDGSGDFILTASGIRRFRPDGTPVLGFQ
                     VGQTNSVDDTDKLLCMLDGDTIVGGTFTTYNGTSRGHIVRINRDGSQQ"
     gene            complement(6345..7034)
                     /locus_tag="VFH05_09570"
     CDS             complement(6345..7034)
                     /locus_tag="VFH05_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET6678506.1"
                     /translation="MPNSEIHNTHRVGWLRAAVLGANDGIVSTASLVLGVAAAGTSSK
                     GILIAGIAGLVAGAMSMAAGEYVSVSSQADAERADLDREQKELATRPTQEHKELTGIY
                     VERGLDPGLASNVATQLMAHDALGAHARDELGISDAFTARPIQAALASAITFSVGAAL
                     PLVIVLLVPVSALMWVVSGSALLFLALLGSLAARTVGASMVTAAARVAFWGALAMALT
                     AGVGALFGVVA"
     gene            7630..8028
                     /locus_tag="VFH05_09575"
     CDS             7630..8028
                     /locus_tag="VFH05_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678507.1"
                     /translation="MFMYLFVLPIVMGFPHSSFAQFVDKGEYVEVAKGTKLCSNIILE
                     KSKGKLDPEKFFWRVTSNVRANGTQTIAAFVLETVYDRQSGKTTIKENRYDQDYATFF
                     KRDPEHEGMLLMISPQDGRVEAIVEMCKKK"
BASE COUNT         1867 a         2284 c         2131 g         1782 t
ORIGIN      
        1 tacgaccggc ctttacaatt tatgcagata tatcgcagca ccgaagtccc atccttcagt
       61 ggaaccacac ggtaggcaac agggcaccat tacactcaca gcactgcagc ggatcacttt
      121 tgtgatttcc ataattcttc cgtccaaaaa gcaaagggcc ggtcctttat gaggaccagc
      181 cctttgctaa ttccacttca cttcaaagag aggggttcag gcttgccggg aaacctttct
      241 ccaaatcaca agcccgctta accccgcact caataggatc aacgtgtcag ggccaggaac
      301 tgaccgaccg ttggttgagc taccgttcgt attagccgta tagttagtgt tctggctaga
      361 ctgaccgttc ccattcttat cccaaccata atggttgcca ttattgctgt tctggttact
      421 gttcagctgg cccaagtggt ttccattatt gctgttcgag tttgagttca aatactgact
      481 aagaggtcct ttccatccgt ccgcgtgcgc taacggagct atacccaaca caccgaccag
      541 agacaggacg gccaacctcg cgattctcat cttgttacct ccctgcttta cgagaagatt
      601 gattgcagat gtccttaagg taatgccggt aagggttcac tggcaattgt ggattatacg
      661 tagtccctca actaagtgag ttttggggtc gctatcatcg gtgcttgatc tcatagatcc
      721 aggcgggatc gtcgaaaggc tcccattgtt gaatcaacgc attaggggtg ggaatgccag
      781 gagatattgt atcaaccgcc ttaggcagtc agcttctggt aagcccaagc acgcataact
      841 aggatattcc ctagtcacaa cattttttcg agcttcgtat actgcacgcg catctgtggg
      901 gcttgtagca atgactacac cgcgtccgag ggaatgcgaa ccctctcgat atcgtcatgg
      961 ctgtgacccg ccaaaaacga cacacgttgt ttactcctct gactgcacga gcctcgacat
     1021 tgtaaggcca ccacgagcgg caacctctct agcttcaaca cagtccctca ttcggttagt
     1081 acagttacag cccctcgctc ttacccttag tcttttgatt tcgtccaaag caaggaagta
     1141 atgaatgaag aaaggagatc ttcgatgtta tccaccatta ctctcgtggt tccatgtgtt
     1201 gagaaggaat ccgtttttct ccaacttgct attcaagcag ctattcaagc gcatcgtgat
     1261 cacccgaaga gttacgaagt gaaggaaggc actgtcaaca acgaccgcag tggagagttt
     1321 tcgcttcctt tataacgacg cacctacgct tcgcagccat gaactctggt ttgcatcaag
     1381 gaccaacctc ctcacgacca cgggcggctc aggctgtcaa cgatgtctgt ccaggcatgc
     1441 tcacagctct tatcggaagc ttggtcgcag ggagcatcaa gccaccatca atttgcgtat
     1501 ggcagcgtgt gccccgaaag cggtgggaag atcgccttct aagctacttg gggtggtatc
     1561 catggcggtg caagcggtgc cggactcgca cctatttccg ttcccgtttt taagaccgct
     1621 acatcgaata tccaaaccgt ggagtctcac atccgtgaat ttggtcttgt acgatccgat
     1681 atcacgagcc ttggcaaggg gaagcgggca acctcatgca cgatgacctc aaggcggaaa
     1741 accagtgtgc aaaaacaccg ctgtagcccc ttgggtacgg ttcctcgtac aggaaaagat
     1801 ctcaatgagg cgcggatact caatcggtaa tatcgtttgt gcaatcctct tgtcttgtct
     1861 ttacgttctg gggttgaccg gctgcggcgg cgacagtggt cctacccctg gaggtttgtc
     1921 gatcaccacc acctcacttc ccgaggggga ggacaacgag ccgtactcgg cgtcactcag
     1981 cggttcggga ggcacgctgc cgtacaggtg gttcgtatcg ccggcccttc ctgccaatct
     2041 ctcgttggat acggcgaccg gcgcaatcac cggcacaccc acgacgcagg caaccacgac
     2101 ccacacgttt acgctgcgcg acagctcggt cccttccgaa accgtccaac aaacgcttcg
     2161 cttaacgatt cgtccggtcc cagcgtttct gggaattatc accgcttcgc tgcctaacgg
     2221 gacggtcaat caagcctata accgccccgt gcaagcgagc ggcgggactc caccgctgac
     2281 ctggaacatc gtcgcaggga cagggacact tccaccaggc ctcaatttga attcgacgac
     2341 cggcgcgatt tcaggcacgc cgactgcggc aggcacttcg tcatttaccg tccgcgttac
     2401 agacgcgcgt ggagaatcgg acacggaacg tctgtcgatc acgatcggcg cagggcccgc
     2461 ccctccgaat ccgccgaata tcacgacgac cacgctcccg gcaggaaccg tcggtcaacc
     2521 ctatagccag cccgtgcagg tgaccggcgg gaccggtgca ctgacttgga gtatcagcgc
     2581 agggaccctc ccggcggggc tcgagttgaa tcaaataaac ggcgcgattt caggcacgcc
     2641 gactgcggca ggcacctcgc cgtttaccgt ccgggtcgac tcaggtgggc aattcgacat
     2701 gcaagcgctg tcgatcacga tcaacgaacc cgccccgccg ccaagtccca gtattacgac
     2761 cacgacgctg ccggcaggaa cggtcggtca aacttataac cagaccctgg gggcaaccgg
     2821 gggaacagga ggactcacct ggagcatcgt agcagggaac ttgccagccg gactgaattt
     2881 ggatcaaccg agcggcgtga tttctggaac accgatcgta ccggcgggcg catcgtcgtt
     2941 taccgtccga gtggcagacg cagccggaca agacgacacg caggcgctgt cgatcacgat
     3001 taacttaccc gctccgccga acatcacgac cactatgctc ccaggaggaa cagtcggtca
     3061 gccctatgct cagacgctgc aagccacggg aggtatagga acactcacct ggagtttcac
     3121 tggttcgctt ccagcgatgc tttcctttag tccgggtggt gtgatttcag gcactcccac
     3181 caatacggga acctcaaatt tcacagtaac ggtcagagat acattgaatt tgtccgacac
     3241 ccagcctctc tcgattacga taagcgcggc caatcctgcc cccgatttag tgaccctgtc
     3301 tgtgaacaca tccggccctg gtgaaggcat agtgaccagc aatccgggtg gcatttcttg
     3361 cccttccacc tgtgtagcgt ctttcccccg cggaacgatc gtcaccttga cggcaacggc
     3421 caaccaaggc tcgactttcg atgaatggcg aggagggcct tgtaacaatc gctcagggac
     3481 ctgccagctt acaatgaatg ataatcagtc ggcgaacgct cggttcgagg acgaggacga
     3541 ctgaggacgc gcaggcgctc aaaggaacaa gggaggtgca aaagtgcgac gtcgccatgc
     3601 tagtgacggt tgcgaatctc ccaccgctct gcttcttgaa tcagcatcga ggttttaacg
     3661 aaaggctcgc tccaaggggg cgggcctttt ctttttggag taaagcacat ggctgggttt
     3721 tcaatagcag cgcctcataa gctctggaga tcccaatcaa ttaagtgctg acgggaatac
     3781 ctgaatcgtg ggtcaatggc tccgtatatc gagctttatg ccccctggtc tctttcatca
     3841 cctactgagc aattttgaac agcctgcgat ctgccttctc gatatgctgg ccccggttgt
     3901 ctgaacatga agaaaggcag tcatgacctg ccgagtggct cgatcgttcc aaactacacc
     3961 gtcgacacct tgtgagaaag gccgtggcgc catggactac tcgtctctct acttcaccaa
     4021 agaaattcag gactaccttc actgttgcga gaatcttttg gcagcactga gtgcacccaa
     4081 caccgcttct cggatctcga tggaagaacg ggaaatgctt gggtactccg tgacagaact
     4141 ccagaaggtc ctcctcgtct gggataaagc agtaagcacc aaggcttcca cagacgcatg
     4201 tatagtggcc gtatgaatga cgcagaaaaa gagactgaga agctaatcgc tcatttcatc
     4261 tccgccggct gagatttttc tctcccctca tccttcatcc tgatctgcta ccaccgctgc
     4321 taccaggaca ggacaactca gccccatctg tacccacttc gatgtcagcc tggtttgttg
     4381 tttcttgtgg ggttaggttg tctgaggttg gactttcgga atttcgtaaa ccgcaggtcg
     4441 caccgaaatc acgaacaaca gcttcaacgt cggtaactgt aacatcccca agcgtgcggc
     4501 tgtcctatat aggacagcca aactgtaact cgttgtaaat gcctgtcact catcggacgg
     4561 ttcggaacaa tcatcgtgtt gattttcttc attagtggtg gtgcttttaa ggcgagggcc
     4621 ctgggttcga gtcccaggcg gctcaccgtt tgatgtggac gcagagacag tgcgaccata
     4681 cctgatccgg cactgctagc tcaccctctc tgcttaactc accctctaaa tgtggtctgc
     4741 tttcagttcg atcgagtgct gctaccctgc tacaccaact agggtcaaca gccggcttcc
     4801 ctagtgccaa ccctaatagg gaaggaatca aaacacctgt aagcatactg gaggttcgcc
     4861 tagtccaccc atggacgaaa gcgcctcgaa aacatttttc acagaattga tgtttacagc
     4921 tttatatcgc accgcggcag tcctgctaac tcttttcctc agtctttccg gctgcagttc
     4981 cggaggcgac acgcagtcag cctccacgac cacaacagga accagcccag cggcaaacga
     5041 tcccagcacc cctgcgacac cgaatactgg tcctgaaacg ggcggctctg aaagcaccgg
     5101 ctctgatggc actattccgg tccatcaact cgccgacctt aacggcgacg tcacagacct
     5161 cgctttacat cctgatggcg atatcgtcgt cgccgggacg ttcagcacct atggtgggca
     5221 gccagtcccg catgtcatca agcttcggcc ggatggctcg ttggctccct ggacgcttgg
     5281 tgtgtctctt atcagtagcc tgcccattga gaccgctatc tctccagacg gaaaggtctg
     5341 gattgcgcag cgtcaaggtg aggtggcgcg actgtggcgt gtctctgacg atggaacact
     5401 ggacagcggg tttgtgccgg tcgacttttt gttcgatgag acgggcgggg gcgtcattcc
     5461 ctcccaagtc ttccagttag agccgatcgg taacggtgtg tatgtcgtcg gtaccttcac
     5521 gagagtgaac gggcagccac ggcgtggact tgtgcgaatt tcggcgacgg ggcaggtaga
     5581 cggcggattc aatgcagaca tcgcgtcatc agcgtatcgg ctggctccga acccggacgg
     5641 cacactttac gtcacaaact tcgagcgact ggacgtgagc gtggtgggca gtcagatcct
     5701 gtatcgattg aacgcagacg gctcccgtgt cagcgggttc tcgcccgtga atgtctatac
     5761 cgttggattt aacgcccgaa tctatgcgct tgcccgagtg ggtgatgaca ccaatgatgt
     5821 gctgattggt ggcggattcg tgctcgacta tagtggaacc gtcaaccccc ttttggaccc
     5881 gacggccatt cacaatctgg cgcgcgtgaa cccggacgga agccgggatc gtgtgcagcc
     5941 acggccgcag ctcggcacgg actcgactaa tacgccaatt gtccgcgcca tcgcgaaaac
     6001 gattgatgga agcggcgact ttattctcac cgccagcgga atccgccgtt tccggcctga
     6061 cgggacgccc gtacttgggt ttcaggtggg ccagaccaac agcgttgatg acactgacaa
     6121 gctcctatgt atgctggacg gggatacgat cgtggggggg accttcacca catacaacgg
     6181 gaccagccgg gggcatatcg tgagaatcaa tagagacggg tcacagcagt agagctttgt
     6241 gcggaagaaa cttgagctaa tgtcaaggcg tcgcaaacac ttgggaacgt gtcgtccagt
     6301 aaacggggca gggccgtcag agcgtgggtg tcgttgctag gatctcaagc cacgacccca
     6361 aacaacgcgc caactccagc ggtcaaagcc atggctagtg cgccccaaaa ggccacccgt
     6421 gcagcagcgg tgaccatcga agcccctacg gtgcgtgcag ctaagctgcc gaggagcgca
     6481 agaaacagga gggcactgcc ggataccacc cacatgagtg cggagaccgg taccaacaag
     6541 acaatcacaa gaggcagggc agcgcccacc gaaaacgtaa tggccgacgc caatgcggcc
     6601 tgaatcggtc gtgcggtgaa ggcatcggat attcccagct catcccgagc atgggcgccg
     6661 agtgcatcgt gcgccattaa ctgagtggcg acgttagagg cgagcccagg atcaaggccg
     6721 cgctcaacgt aaatgccggt cagttctttg tgctcctgcg tggggcgggt cgctaattcc
     6781 ttctgttcgc gatccagatc ggcgcgctca gcgtcggctt gcgaactcac cgacacatat
     6841 tcgcccgccg ccatcgacat cgctccagcc accagaccgg caatgcccgc gatcagaata
     6901 ccctttgaac ttgtccctgc tgctgctacg cctaatacaa ggcttgccgt tgaaacaatg
     6961 ccgtcattcg cgccaagcac agccgcacgg agccatccga cgcggtgggt attatgtatt
     7021 tcagaattcg gcattatccc ttctgctgca agtagtaagt gctcggcttc agagttcgtt
     7081 gtgcgggcca gccattcaag agggctggag tcccaacaac ctgtcggcaa gtctactgca
     7141 aagtcagaag cgtatccatt aaccacagtg agcgacggga tacgctgatc atctctgttg
     7201 ccggattcca gaatcaccca aggagttctg ctcgaagtag tccagcaagg caggattctc
     7261 caaagaaagg cacccccttc cagctcgagg ggcagttcca gtcgtccagc cttggaatca
     7321 cccacctcag tatgttctga tgcctgacag aatttgcatg tgaatgccac tctgcaacgg
     7381 gatgaccagt aacaggtatg ttcaactccg tggaattctc atgaaaatga gaggtcgaat
     7441 tcatgcattc acttaagcaa cactgggtat tacgggtcag aagagcgggt atgccctgca
     7501 ctctaatgta caatcgagat gccgtggcgg gttcggtcta tgcatgactc actcgaagga
     7561 gagacagtga gtattgtcga tggtggtcaa gaatgcaatc tcttcatcgc ccttcaggga
     7621 ggctgatcta tgttcatgta cctatttgta cttccaatcg tcatgggatt cccccactca
     7681 agcttcgctc aattcgtgga taagggcgag tatgtcgagg tggccaaggg cacgaagctc
     7741 tgttcgaaca ttattctgga aaaatccaag gggaagctcg atccagaaaa attcttttgg
     7801 cgcgtcacga gcaacgtgcg ggccaacggc acgcagacca tcgcagcatt tgtccttgaa
     7861 accgtgtatg accgccagag cgggaagact acgattaagg aaaaccgtta tgatcaggat
     7921 tatgctacgt ttttcaaacg agaccctgag cacgagggga tgcttttgat gatcagtcca
     7981 caagatggca gagtcgaagc gattgtggag atgtgtaaaa aaaaatagag cacatcgcct
     8041 ggcaggatgt ctgaatggaa ttgc
//
LOCUS       DASSCQ010000140         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_124975, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000140 DASSCQ010000000
VERSION     DASSCQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124975"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VFH05_09580"
     CDS             <1..824
                     /locus_tag="VFH05_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET6678508.1"
                     /translation="GLVLTGLVEWVGGEIFDVQTFLFHIGGLVFLLGFPYLGLVLGAR
                     FGKEKLPSPQQKFFELDGNTVCPKVLDTSVIIDGRVADLCETRFLEGTFLVPHFILDE
                     LQHIADSSDSLKRARGRRGLDILNKIQKMGDVDVRIIDEDFPHVKEVDAKLVVLAKKM
                     NAKIVTNDLNLNKVAELQGIRVLNINELCNALRPVVLPGEAIRVFVLKEGKEAGQGVA
                     YLDDGTMIVVDNARRYIGKNVDVIVTSVLQTTAGRMIFTRLKEETEREELQITRG"
     gene            864..1607
                     /gene="ispD"
                     /locus_tag="VFH05_09585"
     CDS             864..1607
                     /gene="ispD"
                     /locus_tag="VFH05_09585"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET6678509.1"
                     /translation="MAGRPHIQPVSGQAVRTVALVPAAGLGLRMGGSVRKQFRTVGGF
                     PLVVHALRVLQSSPIIDGIVLAVPATDLHYCRTDIVEAHGFTKVLHVVAGGQERQDSV
                     RQALAVVDDRVDIVVIHDAVRPCVTELMVKQVVEAAKAEGAAIIALPMRDTVKQVGPD
                     GLIERTIDRKPLWLAQTPQAFRRDWLQDAHRKAHAEGLAATDDAYLLEWMGHPVKVLE
                     GSGENIKVTRPEDMILGEAILSSRAAQGT"
     gene            1638..2111
                     /gene="ispF"
                     /locus_tag="VFH05_09590"
     CDS             1638..2111
                     /gene="ispF"
                     /locus_tag="VFH05_09590"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET6678510.1"
                     /translation="MRIGYGYDVHPLGPGRKLILGGIDIPHSKGLLGHTDSDVLVHAV
                     CDALLGAMGEGDLGRHYPSSDSKYKGLSSLKLLEDVMTKLKAKGYRVGNIDTVIVAQA
                     PRLGPHLAAMQKKMAEAAGLDPDQVNVKVKSGEGLDAVGKEEGMTAHAVCLIEPA"
     gene            2131..2781
                     /gene="cysE"
                     /locus_tag="VFH05_09595"
     CDS             2131..2781
                     /gene="cysE"
                     /locus_tag="VFH05_09595"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET6678511.1"
                     /translation="MFKAIRQDLKAVFDRDPAATSALEVVFTYAGFHALIAYRLSHWL
                     KSYGVPLVPRVISQVARWLTGIEIHPSARIGTGFFIDHGMGVVIGETAEIGDFVTLFQ
                     GVTLGGTGKERGKRHPTLGNHVVVGAGAKILGGITIGDNVKIGANSVVLKNVAANSTV
                     IGVPARVIKTQGERLPDATMDHINLPDPISDRFLALEQELIELRKKLENRDDSLPR"
     gene            complement(2889..>3231)
                     /locus_tag="VFH05_09600"
     CDS             complement(2889..>3231)
                     /locus_tag="VFH05_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678512.1"
                     /translation="HLVGSHDFSSFEGTLTDNEDPVCHLRQLSLTRREDRLTIHAYAD
                     RFLKHMVRAIVGTLVEIGHAKRTPESLRKILAARNRTAAGRTAPPQGLFLLRVDYDPE
                     LDSAPADPPTT"
BASE COUNT          677 a          889 c         1011 g          654 t
ORIGIN      
        1 cggggctggt attgaccggc ttagtcgagt gggtgggagg tgagattttc gacgtccaga
       61 ccttcttgtt tcacatcggc ggattggtgt ttctcctggg atttccctac ctggggctgg
      121 tattaggggc gcggttcgga aaggaaaagc tacccagccc acagcagaaa ttcttcgaac
      181 tcgatggcaa cacggtgtgt ccgaaggttc tcgatacgag cgtgatcatc gacggccgag
      241 ttgcggatct gtgtgaaacc cgttttctcg aaggaacgtt cctcgtaccg cattttatcc
      301 tggacgaact tcagcatatt gccgattcgt ctgactcgct gaagcgggcc aggggacggc
      361 gcgggctcga cattctcaac aaaattcaga aaatgggcga cgtggatgtg cggatcatcg
      421 acgaagattt tccacatgtg aaagaagtcg atgcgaagct cgtcgtgctg gcgaaaaaaa
      481 tgaatgccaa gatcgtgacc aatgacctga acctcaacaa ggtggcagag ctgcaaggca
      541 ttcgcgtcct caacatcaac gaactgtgca acgccctccg gccggtcgtc ttgccgggcg
      601 aggccattcg agtgttcgtc ttgaaagaag gcaaggaagc cggacaaggg gtagcctacc
      661 ttgatgatgg cacgatgatt gtcgtggaca atgccaggcg gtacatcggc aagaatgtgg
      721 atgtcatcgt caccagcgtc ctccaaacga ccgcgggccg gatgattttt acgcgcctca
      781 aggaagaaac cgaacgagag gagttgcaga ttacgcgtgg atagctgcga accctcacat
      841 ccaccgcagc tacggcgctg actatggccg gccggcctca tattcaaccg gtctcaggcc
      901 aggcagttcg cactgtcgcc ctcgtacctg ctgcaggact ggggctccgc atgggcggct
      961 cggtgcgcaa acagtttcgc accgtcggag gatttcccct cgtcgttcat gcgcttcgtg
     1021 ttcttcagtc ttcccccatc atcgacggaa ttgtcctcgc ggttccggca acggatctgc
     1081 actattgccg cacggacatc gtcgaggctc atggcttcac caaagtgctg catgtggtcg
     1141 caggcgggca ggagcgccag gactccgtgc gccaggctct ggcggttgtg gatgaccggg
     1201 tggacatcgt cgtcattcac gacgctgtcc ggccctgtgt gacggaattg atggtgaagc
     1261 aggtcgtgga ggcagctaaa gccgagggcg ccgcgatcat tgcgctgccc atgcgggata
     1321 cagtgaaaca ggtaggaccg gatggtctta tcgagcggac gatcgaccgg aaaccgctgt
     1381 ggttggctca gacgccgcag gccttccgtc gggactggct gcaggacgcg catcgcaaag
     1441 cgcacgcgga aggactcgct gccaccgatg atgcttatct cctcgaatgg atgggccatc
     1501 cggtcaaggt cctcgagggc agtggagaaa acatcaaagt caccagaccc gaagacatga
     1561 ttcttggaga ggcgattctg tcttccaggg cggcgcaagg tacatagacc cgatcgatgg
     1621 ggaacgaagg gagacatatg cggattggct atggatatga cgtgcacccg cttgggcccg
     1681 gacgcaaact gattctgggc ggcattgaca tcccacacag caagggtctg ctcgggcata
     1741 cggattccga cgtccttgtc catgccgtgt gtgacgcgct gcttggagcc atgggggagg
     1801 gcgaccttgg acggcattat ccgagttctg attcgaagta caaaggcctc tcgagtttga
     1861 agttgctcga agacgtcatg accaagctta aggcgaaggg ctaccgagtg gggaacatcg
     1921 atacggtcat cgtggcgcag gccccgcgtc tggggcccca cctggcggct atgcaaaaaa
     1981 aaatggcgga agcggcgggc ctcgatcccg atcaggtcaa cgtgaaagta aagagtggtg
     2041 agggcctcga tgcggtcggc aaggaagaag ggatgaccgc ccacgccgtc tgtttgattg
     2101 agccggcgtg accatcgtaa aatagcggac atgttcaagg cgattcggca agacctcaaa
     2161 gctgtgttcg atcgggatcc ggcggccacc agcgccttgg aagtcgtctt cacctatgct
     2221 ggtttccatg cgttgatcgc gtaccggctc tcccattggt tgaaatcgta tggcgtgccg
     2281 ctcgtgccgc gagtcatttc gcaggtcgcc cgctggctga ccggaatcga gatccatccc
     2341 tcagcgcgca ttggcaccgg cttctttatc gatcatggca tgggtgtcgt gattggggag
     2401 acggccgaga tcggggactt tgtgacgctg ttccagggcg tgacgctcgg cgggacggga
     2461 aaagaacgag gcaagcgtca tccgaccctc ggcaaccatg ttgtcgtcgg cgcgggcgcg
     2521 aaaattctgg gaggtatcac gatcggcgac aacgtgaaga tcggggccaa ttccgtcgtg
     2581 ctcaagaacg tcgcggccaa ctccacagtg atcggtgttc cggctcgcgt gatcaagacg
     2641 caaggcgagc ggcttcctga cgccacgatg gaccacatca accttcccga tcccatcagc
     2701 gaccggtttc tggcgctcga gcaggaactg atcgaactcc gcaagaaact cgagaaccgc
     2761 gacgactctc taccccgcta gcgacagtcc cacagaggcc cgccggattg tccgattccc
     2821 agctggccca ccttttacat ttccgctcgg tcaaggcgta ccgcctgagg gcaccgtctg
     2881 tcgctgtatt atgtggtcgg gggatcggcg ggtgcagaat caagctcggg gtcgtagtcg
     2941 acgcgcagga ggaaaagccc ctgggggggg gccgttctgc ctgcagccgt tcggttcctt
     3001 gcagccagga tctttctcag gctttctggc gtccgtttcg cgtggccgat ctcgacaaga
     3061 gtgccgacaa tcgcgcggac catgtgtttc agaaaacggt ctgcgtaggc gtgaatcgtg
     3121 agccggtcct ctcgccgcgt caaagacagc tgccgaaggt ggcagaccgg atcctcgttg
     3181 tcggtcaagg tgccttcgaa ggaagaaaaa tcgtgcgagc ccaccagatg a
//
LOCUS       DASSCQ010000141         5551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1366309, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000141 DASSCQ010000000
VERSION     DASSCQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366309"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFH05_09605"
     CDS             <1..223
                     /locus_tag="VFH05_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678513.1"
                     /translation="SGPFRVGPGTYGLVAFEDVKRGRRVSTHRTVPPLDNDSRGRQSG
                     GAERGKIKRFLQAAGKDEPAKKLWEHQRA"
     gene            334..1203
                     /locus_tag="VFH05_09610"
     CDS             334..1203
                     /locus_tag="VFH05_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678514.1"
                     /translation="MSHSVLGRKALTLLIISTVVLTLTPSVHAEEKTDTPFHDKLMIR
                     GGWAYVFGATTDVSIGGPVLGVGANVDFTRTLGGDTSTDAFRLDGLYRFNDRHAIGLS
                     WYRVGLGGEKILNQQVQIRDQIVAAGASTQSSLSFNIYRLLYNYSFYRNDKVELGFSP
                     GLYIAQTKFNFAGQGTITPPGGVPTPGSTTIVNEQVTLPLPSVGFLANYNITPKLQFQ
                     SRFDFFYLAVNEYTGSMFEIYLGLEYRLHQHFAMGAAYDRLTTELSGSGDEGFNVNFG
                     YNLAYVYATLYLF"
     gene            1348..3153
                     /locus_tag="VFH05_09615"
     CDS             1348..3153
                     /locus_tag="VFH05_09615"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6678515.1"
                     /translation="MRNLTRPLYVAVIGATALILGTIWLTPLGAEAESGQPSLKEIID
                     KRSKDDAERTAAGVPDDPLGRGTPRSSVRGFLLSAKDRNYAQAAEYLDLRNLPPGMTE
                     SQGPELARQLRIVLDRTLWIDLELLSTNPEGDQSDNLPVVRERVGRISAEGKTYDLLL
                     QRVPRGDGVYIWKFADVTVADIPELYQQVGYGQLERIFPAWMFDVSVLGINLWLWAAV
                     IALAIVLYPAAMLVTRLAAAALRLLHVQFAEQFERLFSGPITLLIWTVMVRSAGTFLG
                     SSIALGALSQARTVQMIGLAWLLMRVVDFMAQRAGANLERKGLGGSRVLLMPIARLIK
                     FVALAGAVLLWLDNAGYKVTTLLAGLSISGVAVALASQKSLENLFGAVTLFTSRPVKV
                     GDFCGFGDKVGFVEEIGLRATRIRTLERSIITVANAEFANLHLDNYSARDRFWYHPTL
                     QLRYETTPDQIRYILVEVRKMLYSHPKVLPEPLHVRFKSFGQYSLDVEVFAYIGVLDY
                     GESLEVAEDLNLRMMDIVTAAGSDFAFPSEVQYSLPGKPLDEERAKAVSQQVKEWKAK
                     QELYLPNFPADKIAAIKNSLEYPVQGSPQFSPARG"
     gene            3246..4649
                     /locus_tag="VFH05_09620"
     CDS             3246..4649
                     /locus_tag="VFH05_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6678516.1"
                     /translation="MRRRLRSVCTCLLMLLLLAVHAGCEYVRPTLNAPLEKWEPGGGY
                     RFTNVAPPGGNNSDSFLFIAAFSGGGTRASSLAFGALRELANQQIVWEGKQKRLLDEL
                     DLIHALSGGTFTGGYYALYRDQIFHDFEYRFLRKDWESELRHRVFRSPSNWVRLWSPY
                     FGRAHLMAELLNEALFDKKTYGDLSALQQRPMLIIHASDMATLARFEFTQFQFDFLCS
                     DLSQLPIADASAASAALPLVLSPISLKNYANQCDFHAPDWLAQAKGGGRIGAQRANEL
                     LSLTDAEKRPYIHLLDGGLSDNLALRGIIEGSGLQGSFEKLLVMGGVKNVKKLVILAV
                     NAETSPDVLEFRSDHIPVISKAMSSMIDIPINRYSFDTTTLINMGVEKWRAELKNKPR
                     PASSPWAPDADIYFINASLSEVVDPDERVALMKIPTTLYLKDEQIDRLVMAASKLIRG
                     NKEFQRLMMDIQAKPNP"
     gene            4778..5254
                     /locus_tag="VFH05_09625"
     CDS             4778..5254
                     /locus_tag="VFH05_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678517.1"
                     /translation="MMSRLMCCMVIGALMAAVPAFSQPVSDTNMEILKEKLKADKKLL
                     VAGNMELTDAEAKQFWPLYDSYQKELDGINKQLGQTIKDYADAFNKGNVPNDTAKKLL
                     GEALSVEEQEVKLKRTYADKLGKVLPATKVARYIQLENKIRSLIRFELAQQIPLVY"
BASE COUNT         1258 a         1496 c         1556 g         1241 t
ORIGIN      
        1 gtcagggccg tttcgcgtcg gcccggggac ctacggactt gtcgccttcg aggacgtcaa
       61 acgcggacgg cgtgtatcaa cccaccgaac cgttcctccc ctcgacaacg atagccgcgg
      121 ccgccaaagt ggcggagcag aacgaggaaa gatcaaacgg ttcttgcaag ctgccggcaa
      181 ggatgagcca gccaagaagc tgtgggaaca tcagcgggca tgaaagaggg cataagagaa
      241 tgacctcgtt cccccctgat tgcatagaca gcctaagcaa ttttcgctag actacgcggc
      301 tttgaatcct cgattccgca tggaggtcgt gtcatgagtc actcagtgct gggcagaaag
      361 gcgctgacgc tgcttatcat ctcaaccgtg gtgctgacgc tcacaccctc tgttcatgcc
      421 gaggaaaaga ctgatacgcc gttccacgat aagttaatga tccgaggggg ctgggcgtat
      481 gtcttcgggg caaccaccga cgtctcaatc ggaggccctg tcttaggggt cggcgcaaac
      541 gtggacttca cccgaaccct tggtggggac acgagcaccg atgcattccg gttagacggt
      601 ttgtaccggt tcaacgaccg gcatgccatc gggctgtcat ggtatcgggt cggcttgggc
      661 ggagagaaaa ttctcaatca acaagttcag atccgagacc aaatcgtagc agccggtgcc
      721 tccacgcaga gcagcttaag ctttaatatc taccgattgc tctacaatta ttcgttttat
      781 cgcaacgata aagttgagct cggattctca ccgggacttt atatcgcaca aacgaagttc
      841 aacttcgcag gtcaagggac cattacgcca ccggggggcg ttcctacacc cggcagtacc
      901 acaattgtga acgaacaagt cacacttccg cttccgtcgg tcggctttct tgcgaactac
      961 aacatcacgc caaaattgca attccaaagc cgttttgact ttttctattt ggccgtgaac
     1021 gaatacacgg gatcgatgtt cgagatctat ctcggactcg aatatcggct gcaccaacat
     1081 ttcgccatgg gtgctgccta tgaccgtctg actaccgagt tgagtggaag cggggacgaa
     1141 gggttcaacg tcaatttcgg ctacaacctg gcctacgtct acgccaccct ctatcttttc
     1201 taaccggcca atcgttgatc cttcagtgaa gaactgtcct caagacacca atctgagaag
     1261 tctacgaatc ccgataaaag aattcgtata acgtctgcct caaaattccg gagtccatat
     1321 gcggactagt ggaaggagga actggatatg agaaacctga ctcgacctct gtatgtggct
     1381 gtcattggag ctacagcact cattctcggg acgatttggt tgacgccgct gggcgcagaa
     1441 gcagagtccg gacagcccag cctcaaggaa atcatcgaca aacggtcgaa ggacgacgct
     1501 gaacgcacag cggcgggtgt gccggatgat ccgctcgggc gggggacgcc ccgatccagc
     1561 gtcaggggat tcctcctctc ggcgaaagac cgcaactatg ctcaagccgc ggaatatctg
     1621 gatctccgta acctgccgcc aggtatgacc gagagtcagg ggcccgaact ggcgcgacag
     1681 ttgcggatcg tgctcgaccg aacactctgg atcgacctgg aattgctgag cacaaatcct
     1741 gagggggatc agagcgacaa tctgcctgtc gtgcgggagc gggtcggacg catttcggcc
     1801 gagggcaaga cctatgacct cctcctgcag cgcgtcccgc gaggcgacgg ggtgtacatc
     1861 tggaaatttg ccgatgtgac cgtcgcagac attccagagc tatatcaaca agtgggatac
     1921 ggccagctgg agcggatttt cccggcgtgg atgttcgacg tctcggtcct cggcatcaat
     1981 ctgtggctct gggctgcggt catcgcattg gcaatcgtgc tctacccggc tgccatgctt
     2041 gtgacgaggc tggcggccgc cgccctgcgt ctgctccatg tgcagttcgc ggaacagttt
     2101 gagcggctct tcagcggtcc gatcacgctg ctgatctgga ccgtgatggt cagaagcgcc
     2161 ggtacctttc tcggatcgtc aattgcgctc ggggcgctga gtcaagctcg gactgttcag
     2221 atgatcggcc tggcctggtt attgatgcgc gtggtcgatt ttatggctca gcgcgctggc
     2281 gcaaacctcg aacgaaaagg tctcggagga tctcgggtgc tgttgatgcc tattgcccgg
     2341 ctgatcaagt ttgtcgccct tgccggtgcg gtgctgctgt ggctggataa cgccgggtac
     2401 aaggtcacca ctctcctggc cggtctcagc atcagcggcg tcgccgtggc gctggcgtcg
     2461 caaaagagcc tcgaaaatct tttcggtgca gtgacactgt ttacgtccag acccgtgaag
     2521 gtcggagatt tctgtggttt tggcgataag gttggttttg tggaagaaat cgggctgcgg
     2581 gctacacgga ttcgtacgtt ggaacggtct atcatcacgg ttgccaatgc cgagttcgcc
     2641 aaccttcatc tcgacaatta ctcagcacgg gaccggttct ggtatcatcc gacgctccaa
     2701 ttgcggtatg agaccactcc agatcaaatc cgatacattc tcgtggaagt tcggaaaatg
     2761 ctgtactccc acccgaaagt gctgccggag ccgctccatg ttcgcttcaa aagctttggc
     2821 caatactccc tggacgttga ggtgttcgcc tacattggcg tgctggatta tggcgagtcg
     2881 ttggaagtgg ccgaagatct caatctgaga atgatggata tcgtgaccgc tgccggatcg
     2941 gattttgcgt ttccatccga ggtgcagtac tcgttgccag ggaaaccgct cgatgaggag
     3001 cgtgccaagg ccgtgagcca gcaagtcaag gagtggaaag cgaagcagga actctacctg
     3061 ccaaactttc ctgccgacaa gattgccgca atcaaaaact cgttggagta tcctgtccag
     3121 ggctcgccac aattttcacc ggcccggggg tgacggaatt ggtggaccca cgataagact
     3181 ttggttgaga tgagaaagcg cccaggttct ggtacaacgc tgttcaggcc atcggaaagg
     3241 acaacatgcg aagacgtttg agaagcgtgt gcacttgcct gctgatgctc ctcctgctcg
     3301 ccgtccacgc gggatgcgag tatgtgcgtc cgaccctcaa cgctcccttg gaaaaatggg
     3361 agcctggcgg cggatatcgt ttcaccaatg ttgccccgcc agggggaaat aactcggaca
     3421 gttttctctt tattgctgcc ttttcaggcg gaggtacccg cgcatcaagc ctcgcgtttg
     3481 gcgcgcttcg agaactcgcc aaccagcaga ttgtctggga aggaaaacag aagcgtctgc
     3541 tcgatgaact cgatctgatt catgccctgt ctggaggcac gtttaccggc ggctattacg
     3601 cgctgtatcg cgaccaaatc tttcatgact ttgaataccg cttccttcgg aaggattggg
     3661 agagcgaact cagacaccgg gtctttcggt cgcccagtaa ttgggtccgg ttgtggtcgc
     3721 cctatttcgg ccgggcgcac ctcatggccg aacttctgaa tgaagcactg ttcgataaga
     3781 agacctatgg agacctctcg gccctccaac aacgacccat gctcatcatc catgcgtctg
     3841 acatggccac cttggcgcga tttgagttta cgcaatttca gttcgatttc ttgtgttccg
     3901 atctcagcca attgccgatc gcggacgcct ccgcggcctc tgcggcgctt cctcttgtac
     3961 tcagtccgat ttccctgaaa aattatgcaa accagtgcga ctttcacgcg ccggattggt
     4021 tagcgcaggc caaggggggc ggtcggatcg gtgcacagcg tgccaacgaa ttgctctccc
     4081 tgacggatgc ggaaaaacgt ccctatattc atttgctcga tggagggctc tcggacaatc
     4141 tggcgctccg aggaatcatc gaaggcagcg gcctgcaagg atcgtttgaa aagttattgg
     4201 taatgggcgg cgtgaagaac gtgaagaaat tggtgattct cgccgtcaat gccgaaacgt
     4261 cccctgatgt gctcgaattt cggagcgacc atattcctgt catctccaag gcgatgagct
     4321 ctatgattga tattccgatc aatcggtatt cgttcgatac gacgaccctc atcaatatgg
     4381 gagtggaaaa gtggagagcc gagctgaaga ataaacccag acctgcctcg agcccctggg
     4441 ctccggatgc cgacatttat ttcatcaatg ccagcctcag cgaagttgtc gatcccgacg
     4501 aacgtgttgc cctgatgaaa attccgacaa cgctgtattt gaaggatgag cagatcgatc
     4561 gcctggtcat ggcggcgtcc aagctgatcc gcggcaacaa ggagtttcag agactgatga
     4621 tggatatcca ggcgaaaccc aacccttgac caccgattgg gcaggccgac cgatgagcgg
     4681 cctgtcactt ttgacagtta caagtgccca tgaaggatgc tatgcatgac cgtttgcttc
     4741 agtacgagaa agtctaaccc aacaggaagg atgaatcatg atgtcaagac tgatgtgctg
     4801 tatggtcatt ggtgcactaa tggcggctgt tccggccttt tcccaaccgg tctccgatac
     4861 gaatatggag atcttgaagg aaaagctcaa agcggacaag aaacttctgg tcgccggcaa
     4921 catggagctc accgacgcgg aggccaagca gttctggcct ctctatgatt cctatcaaaa
     4981 agagctcgac ggcattaaca agcagcttgg tcagacgatc aaggattatg cggacgcctt
     5041 caacaagggc aatgttccca acgacacggc gaagaaactg cttggcgaag cgctctctgt
     5101 cgaggagcaa gaggtgaagt tgaaacgcac ctatgccgac aagctcggaa aggtgctgcc
     5161 cgcgacaaag gtcgctcgct atatccagtt ggaaaataag attcgatccc tgatcagatt
     5221 tgagttggcg cagcagattc ccctggtcta ctagatccga acaggtgaga cgccggtccg
     5281 tcatcctctg cgtcaatgag acgagatgcc ggactctcgc gtcatgcacg gggcggcagc
     5341 ccaagccttt ggagtctgca gaataccggg gtaatggagc gtatgactaa agccgattcc
     5401 gctgatcttg ctctgttgca acaagtattt catccttcag attttacgcc ggcgagcgag
     5461 gtggcgttta tccacgcgtt gaaagcggct ctggtcgcca aagcggagct caccattctg
     5521 catgtctcac ccaagcacga attggagtgg a
//
LOCUS       DASSCQ010000142         5099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1537803, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000142 DASSCQ010000000
VERSION     DASSCQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537803"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2405)
                     /locus_tag="VFH05_09630"
     CDS             complement(<1..2405)
                     /locus_tag="VFH05_09630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678518.1"
                     /translation="MRTDKRSSQRIEKKAPSRVHKRGVVGYSKKTALLEEAITHMNSG
                     KYGRSSAALKELLALDPQNTEARRLFATLHLRLGSLVTARQAFESLANEAIGRQDYWL
                     AESLLGEYLAAGPRCVPFLEQLAYVYQEKGDELAAVGELGKAIEILRDDPDPDNPQKA
                     AQLYSKIRELAPASSVAMQLATLFDVQTGEFLVSPSAQSAAHVDASPSVADISAAAQV
                     DHPLPDVMPWEVTEEASPVPDSEASFTASSAASEDAAVSASPGIEDEDVGPLGQAVSD
                     PVPSTAVDEPPLAFTLKTEETFIDPSVDASNTTTVPSESVSLQESAGTPSHMPWEQMT
                     DASNTTTVPSEPVSLQESAGMPSHMPWEQMADASIRIIEPEPPSPDPGLLESILASLR
                     TEDNGVASEAPVAEVRVPEAEAPEEPMAPAPAPLSDGDIAFVSLSSPMPWEQVSDAAM
                     QIPEEEPAPLSPAEDRPFHIATAEPELAVSSAAVDVEPSPAKPALDQDAAPSSDSTPP
                     PDSPSLPSTDSAPPASFSWNSVFDKAWKFTAGTMALSTPVPSLIGHEPGDQEQPGQAA
                     PPCPETTDDPDLKQSIAIVSSPHLETPLSPLEPAVSASSSAIVEEGAVHESVVEPTSP
                     PAVPSEHWNTGEVAIQVHRPSKKRKRSRHGPEEAEPKASPASPTTESLSDELSQTRRE
                     WISTSNEATPLIEETVSASASPDTRPDWMQATDAITFDRPATPPVATWSDAPAVTASA
                     REAEVPPSAAASAVDVLFARSGPIDEVRPHIPLWSKPAPRLIARLHRIRISIWSFIWS
                     CFST"
     gene            2779..4425
                     /locus_tag="VFH05_09635"
     CDS             2779..4425
                     /locus_tag="VFH05_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET6678519.1"
                     /translation="MTQFRSGGRLARLLRIFPQIPSHAWYGPLRVWVLCVMVMFGLLV
                     PLSVPAQTNGNSRVALDFNDVELSVFVRFISELTGKNFVLDDAVKKAGGKITVFSPTK
                     VTPDQAYSLFVSALEVSRLAVVPRGPVTQIVQMGELPPERGVFVYKLKHANATDLAAV
                     LTNLVARSQTVSQTTPGIRPPIRPLTEFEAPVQVFADKATNSIIISSTKSAYARLQSV
                     IRDLDGRRKQVFVEAVILEVQVDRLRQIGTDPVQVLAAGKSGQFQGIGSINRAPEDIA
                     TIAQTISGIAAGTSTGGAVSVLNTINIRAFLQLLLSLTDTNVLSTPQVLAADNQKAKI
                     VVGENRPFPTGQAQGITGGTLVTIERKDVGVTLELTPQVLENDLIRLEIKQEITAIAE
                     AVAQTIGTGAATVPVGPTTTKRSMETTTIAKDGQTLVVGGLVRDNLTISERKVPLLGD
                     IPLLGWLFRFQSRQSEKLNLLVFLTPSLVRDEVDMVDLNTRKSAELNSLQRENRVEEP
                     TRLKQEVIEKLQRSTIPLQPTQPSQTTEPLVLPPPVSKPE"
BASE COUNT         1088 a         1428 c         1514 g         1069 t
ORIGIN      
        1 gtggaaaagc acgaccagat gaacgaccag atgctgatcc ggatccgatg caggcgagcg
       61 atcaaacggg gagccggttt cgaccacaaa ggaatatgcg gccggacctc atcgatgggt
      121 ccgctccgtg cgaacaacac atcgacggct gaggcggcgg cagatggagg aacctctgcc
      181 tctctagcgg aagccgtcac ggctggtgca tcgctccatg tcgctacagg gggcgtcgcc
      241 ggccgatcga acgtaatggc atccgtggcc tgcatccagt ccggacgggt atccggcgag
      301 gctgaggctg agacagtctc ttcgatgagg ggcgtcgcct cgttggaggt cgatatccat
      361 tcccgtcggg tctgactgag ttcatccgac aaggactccg tcgtgggaga agctggggaa
      421 gccttaggct cagcctcctc cggtccgtgt ctcgaacgtt tgcgcttctt ggaaggccga
      481 tgcacctgta tcgccacctc accggtgttc caatgttcag acggaactgc cggtggagag
      541 gtcggctcga cgacggattc atgtacggcg ccttcttcga cgatggccga agacgaagcg
      601 gacactgccg gctcgagtgg tgagagaggc gtctctagat gaggcgagga tacgattgca
      661 atcgactgtt tcaaatcagg gtcgtctgtt gtttcaggac aaggcggcgc agcttggccc
      721 ggctgctctt gatcgccagg ctcgtgaccg attagtgaag gaaccggggt actcagcgcc
      781 atggttcctg ctgtgaattt ccacgccttg tcaaagactg aattccatga aaaggaggcg
      841 ggcggtgcac tgtccgttga cggcagcgac ggagaatctg gcggaggagt ggaatcggat
      901 gagggagccg cgtcctggtc aagagccggc ttggcagggg aaggctcaac atcgactgcg
      961 gcggaagaaa ccgccagctc gggttcagcg gtcgcgatat gaaacggtcg gtcttcggca
     1021 ggagaaagag gcgcaggctc ctcttcagga atctgcatcg ccgcatcgga aacttgttcc
     1081 cagggcatcg gtgacgacag cgagacgaat gcaatatcgc catcactcag cggcgccggt
     1141 gctggcgcca tcggctcttc gggggcctcc gcttcaggca ctcttacctc tgcgaccggc
     1201 gcctcagaag ccacaccatt atcttcagtt cgcaatgagg cgaggatcga ttcgagcaag
     1261 cccggatctg gagagggagg ttcaggttcg atgatccgta tcgaagcgtc agccatctgc
     1321 tcccacggca tgtgcgaggg catgccggcc gattcttgca gcgataccgg ctcgctcggc
     1381 accgtcgtcg tgttggaagc gtcagtcatc tgctcccatg gcatgtgcga gggcgtgccg
     1441 gccgattctt gcaacgatac cgattcactc ggcaccgttg tcgtgttcga agcgtcaacc
     1501 gagggatcga tgaaagtctc ctctgtcttc aacgtgaagg cgagcggtgg ttcatcgaca
     1561 gccgtactcg gcacggggtc cgacaccgct tggcccagcg gtccgacatc ttcatcttcg
     1621 atgccaggag acgcggatac agccgcgtct tcgcttgccg ctgaggatgc cgtgaaactc
     1681 gcttcagagt cagggaccgg agacgcttcc tcggtcactt cccaaggcat cacgtcggga
     1741 agggggtgat caacttgcgc ggcagccgat atgtcagcga ctgacggcga ggcatcaaca
     1801 tgcgcagctg actgagcgga cgggctgacc aggaactctc cagtctgcac atcgaataag
     1861 gttgccagct gcatggccac cgagctggcg ggtgccaatt ccctaatctt cgagtagagt
     1921 tgtgcagctt tctgcggatt gtccgggtcc gggtcgtctc gcaggatttc gatggctttg
     1981 ccgagctcgc cgacggcagc caattcgtca cctttttcct gatagacata tgcgagctgc
     2041 tcgaggaagg gaacgcaacg aggaccggcg gccaagtact cgcccagcag ggactcggcc
     2101 agccagtaat cttggcgccc aatcgcttcg ttggcgagcg actcgaaggc ttgtctggca
     2161 gtgacgagac tcccaagcct gagatggagc gtggcaaaca atcggcgggc ttcggtattc
     2221 tgcggatcga gcgcgagcag ttctttgagt gcagcggacg accggccgta tttgccggag
     2281 ttcatgtgcg tgatcgcttc ctcgagcagc gcagttttct tgctgtatcc gacgacaccc
     2341 cgcttatgca ctcgtgaggg tgccttcttc tcgatccgct gggaggagcg cttgtccgtc
     2401 cgcacgtgtt aaccttagca gaggttcgat gcaaaccggc tctgacggct ggtttcagcc
     2461 gcggtgcgtc tccgccaacc ggtaccggga tgactcatgg gggaaactgg tgacagatct
     2521 gcaatcctga tgccatcttg cctcactctt tcacggccct ttctctttgt ttatcttatg
     2581 ttaccgcggg tcgtgacagc ggactccgat cagaccactt caaatttgca cagctgtacc
     2641 gagaatgggc aagggacggt cgacgcctga cttgtctggc cggaaaagca ctgaaagccc
     2701 cagttggttg tgtccagcag tgccttgtgt tacggtctgg cccatgcttt tcctccatcc
     2761 actacagggt ctaagcggat gacacagttc cggtccggcg gccgcctagc ccgccttctg
     2821 cggatcttcc cacagattcc gtctcatgca tggtacggac ctcttcgggt ctgggtgctc
     2881 tgtgtgatgg tgatgttcgg actgctggtc cctctgtccg tgcctgcaca aactaacggc
     2941 aattctcggg tagcgttgga tttcaacgac gtcgaacttt ccgtcttcgt gcggtttatc
     3001 agcgagctta cggggaaaaa tttcgtcttg gacgatgcgg tcaagaaagc gggcgggaag
     3061 atcaccgtgt tttctccgac caaggtgacg ccggatcagg cttacagcct cttcgtttcc
     3121 gcgctcgaag tcagccggtt ggctgttgtg ccccgaggac ctgtcacaca gattgtgcag
     3181 atgggagaac tcccaccgga acgcggcgtc ttcgtctaca aactgaaaca cgccaatgcc
     3241 acggatttag cggcggtctt aacgaacttg gtcgcccgct cgcagacggt ctcacagaca
     3301 acgcccggca tcaggccgcc gatcaggccc ttgaccgaat ttgaagcccc cgtccaggtg
     3361 tttgccgaca aggcaactaa ttccatcatc atcagttcga cgaagtcggc ctatgcgaga
     3421 ctccaatcgg tcattcgtga cctcgatggc cgacgaaagc aggtgttcgt cgaagccgtg
     3481 attttggaag tgcaagtcga tcgactccgg caaatcggca ccgacccggt tcaggtgttg
     3541 gcagctggaa agagcggcca gttccaaggc atcggcagca ttaaccgagc gcccgaggat
     3601 attgcgacga tcgcacagac catcagtggt atcgcagcag gaacctcaac gggtggagcg
     3661 gtgtcggtcc tcaataccat caacatccgg gcgttcttac agctcctgct gagtctgacg
     3721 gatacgaacg tgctttcgac tcctcaggtg ttggcggccg acaatcagaa ggcaaagatt
     3781 gtcgtgggag aaaacagacc ctttccgaca ggccaggcac agggcatcac cggcggcacg
     3841 ctcgtgacca ttgaacggaa ggacgtgggc gttacgcttg agttgactcc acaagtattg
     3901 gaaaacgact tgatccgtct cgaaatcaaa caggaaatca ccgccattgc ggaagccgtc
     3961 gcccagacca tcggcacagg ggccgcgacg gtcccggtgg gaccaacgac gaccaaacgt
     4021 tcgatggaaa ctaccacgat agccaaggac ggccagaccc tcgttgtggg cggactcgtg
     4081 cgggataact tgactatcag cgaacgcaaa gtcccattgt tgggagatat tccgctgttg
     4141 gggtggctct tccgattcca aagccgacaa tctgaaaagc tcaatttgct cgtgttcctg
     4201 actccctccc tggtaaggga tgaggtggac atggtggatc tcaatacgcg taagtccgcc
     4261 gaactgaatt cgctgcagcg cgagaaccgc gtggaagagc ccacgagatt gaagcaagag
     4321 gtcattgaaa agcttcaacg gtccaccata cctttacagc caactcagcc atcccaaaca
     4381 accgaaccat tagtgctccc accaccagtc tcgaagcccg aatagtccaa ctcggtctcg
     4441 tgtgttggtt taccacgcga gggtgggttt cggcgtctgg aaagccatgc gtgactcact
     4501 tgctgtaggc gacgttgcgc ttgagagcgc tgtcggtccg gaaactcctg ccgcttcttg
     4561 cataaagaca acataccgta ctgcacttca aaagctgggg gagacactta ttcattcaaa
     4621 tcaagcatcc caacgaagga gccgcaagcc tgtgagaacg cacatttcat tttccgttgt
     4681 agcgatcacc ctcagcctcg tggttgcatg cgtggaccat cggcccagat ttgtcgatgt
     4741 cctcaaggat caatctggag agtcaaatca gcaccaagcg ctgaacgcga tcgacttgca
     4801 gatgacatga gcccaagtca aatcgctgaa gcacgaaaat tggcgcgtga gtggaatgtc
     4861 agaatgagcg cccgacagag ctaagagaga ctgtgatggt ggctgtgaaa ggaagttatt
     4921 tctctttcgg gcgatcaaaa gcggaagcga agataggaag tcttatggca tcggcagcac
     4981 tgccagggat accaccccag gctgctcaga atgtggtgcc tccattgatg acgagccaaa
     5041 cgttttagca cgcattgcct ctgatgcttt gacgtcagcg gaatgaagtg tctctgccg
//
LOCUS       DASSCQ010000143        11756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_755317, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000143 DASSCQ010000000
VERSION     DASSCQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755317"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFH05_09640"
     CDS             <1..733
                     /locus_tag="VFH05_09640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6678520.1"
                     /translation="NIVDCLASVAWAGERIVIDAESSDRTAELARAAGARVIVRPWPG
                     FGAQKNYGMDQVGTEWILILDADERVPPALRDEILGRIGSWKPGEPVAYEIPRRNFFY
                     GAWVRHAGVYPDYQIRLFRRGYARYNDVPVHENLMVQGVIGKLANPLDHFTERSIRDH
                     FRKFGHYTTLAAQEKAGRVRHVGASDLLFRPLVVFVKSYVLKRGYLDGIRGLIVSVFA
                     GMYTFVKYAKLWDMTRSSVGVRDDS"
     gene            733..1872
                     /locus_tag="VFH05_09645"
     CDS             733..1872
                     /locus_tag="VFH05_09645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6678521.1"
                     /translation="MARPILYIHGVAAVGGAERDLLAILRTLARLDWEPHVACPSEGP
                     LAKLMIQAGVVLHPMVFPPWRKWFSPLIRWLAIKKLRTLLEQLKPALIHVNDIWWVPQ
                     TLAAVGGRPGLRIPVVAHVRQEIEPEKVRRYRLHELDGVIAISRHVERALIAGGVDQG
                     TVRTIYSGVELLRSDAGMSDRTAICRSLAVSDEAVLLGTVAHLFPRKGYDVMLKALPG
                     IIRTAPAVHYVIIGTGDGGYEQRLRALSAELGIADHVHFVGFQEDVKPFLGALSCYVH
                     PARMEGFGIAVVEALAAGKAVVATNVGGLPEVVEEGETGLLVAPDDPGALTAAVLSLL
                     RDDGRRRTMGERAVKWVHERFDLRESVAAMEQFYQEVLSAHPNRT"
     gene            1869..2972
                     /locus_tag="VFH05_09650"
     CDS             1869..2972
                     /locus_tag="VFH05_09650"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6678522.1"
                     /translation="MRIGIDASPVAGDRGGVGWHTYYLLRAMLAAPSEARFVAYLRPG
                     MTPPSEASEWAGVDRLEWVTVSKWAMTRRGTADRLDLYHGTNFRMHTVGRFGGIVTIH
                     DLWLERFPEYSSKLLGQRLSSYKTKRIARRAKKVVTVSQFSARELMSVYGLCEQHIAV
                     IPNGVSEEFSPRQDAAEMSILKQRMGLKTDRFILFVGGADPRKNHRKVLEAASLIRGH
                     LDGRTVLLVGSPTHPFGSYERTAASYGLAAHVLCPGRLSREDLRLLYSYADLFVFPSL
                     YEGFGMPVLEAMACGAPVITSKDTALGEVAGDAAVVIDPHDARALADSMIRVLENDSF
                     RVSLREKGFLRVKDYRWSHVAARTLSLYSSLCR"
     gene            2983..4002
                     /gene="rfaQ"
                     /locus_tag="VFH05_09655"
     CDS             2983..4002
                     /gene="rfaQ"
                     /locus_tag="VFH05_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HET6678523.1"
                     /translation="MAPHNILTIKLRYLGDVLLATPTWQALKAAYPRAKLTAIVNRGT
                     EDILLSNPHVDEVLSLERGSLVQQYRFIASIRRRGFDTVVDLTDGDRAALLTWMSGAR
                     VRIGFNAEHRWTGRCYTRVITSDAGAHRIERDLSALAPLAIPVIDRIPRMWLTEEDDA
                     AADDLLRRVGVQSDRRWVVIQPGARYWFKAWPPERFAELADRVADRYGCQVLVAGTPQ
                     EAALAQTVVAQAKHRLISIAGLSRVRTLAALLKRSALFVGNDTGAMHIAAAVGTPVVG
                     LFGPSNPLEWGPRGGPAEVIYKGLDCRECFHPTCRRGEQNCMKLISVSEVMSAAERLL
                     AHGLS"
     gene            4031..4540
                     /locus_tag="VFH05_09660"
     CDS             4031..4540
                     /locus_tag="VFH05_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6678524.1"
                     /translation="MGAILDIGCGANKHEGALGIDCRKVTGVAVICDFEQGLPFREGS
                     VAVVYLHHIVEHMHDLIAFMVELYRVCEPGAKLFVRTPYYASREAFVDPTHVRFITEA
                     TFEYFDYPNYYGLNTNIKIRSIDYKMRKSLRFLPRYFQKRCRQFLWNACIEMDLLLEV
                     VKPLRPRSE"
     gene            4549..5445
                     /locus_tag="VFH05_09665"
     CDS             4549..5445
                     /locus_tag="VFH05_09665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET6678525.1"
                     /translation="MCKVTVVIPVYNGGRYLRESIDSVLAQTFHDYEIVCVDDGSTDD
                     SSVLLQEYGQRLRVVRQENSGQSAARNVGVKLARGEYIAFLDQDDVWYPSKVQNQVAV
                     LDAEPDVVLVHCNFDRIDERGRMLERGAGMIERTSALGSSMGQLIGEALIFPSAMMIR
                     KEGYERIGGFHHALQGFEDFDLIARLKAQGRFVMVEEPGMAYRMHGKGFTRTGGIHVI
                     RSRETFLRRMQTLYRGHPSKQAIVKHMLADCYSDWGMHDVRIGNRQEARAKLLQSIRC
                     NPFKFRTYTRLLRAILPSSRLV"
     gene            5518..6105
                     /locus_tag="VFH05_09670"
     CDS             5518..6105
                     /locus_tag="VFH05_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET6678526.1"
                     /translation="MDLQGQRDLLLLSELDRNGGATQRTLASKLGVALGLTNLYLKRL
                     VRKGHIKIMTIPRNRIRYLLTSQGFAEKSRLTYQYMQYSLSYYRDMRGRLNDMLSTLE
                     WTHGQRVAICGTGELAELAYLALREMNVNCVGFVDGNTRESFLSCPVSSPDGIAGWQL
                     DRVLIADFDHAETYEEQLVRSGIPREKLLRLGLRH"
     gene            6159..6683
                     /locus_tag="VFH05_09675"
     CDS             6159..6683
                     /locus_tag="VFH05_09675"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HET6678527.1"
                     /translation="MSVEPATNQTNSSHWYALRTRSRHEKMVRDQLMQQGIEHLLPTV
                     KRLSQWKDRKKEVEVPLFSGYCFVRFGPDQKLPILKTVGVVDIVGSGQRPEPIPDEEI
                     IAIQTLMASVLPYDPHPYLQEGMLVEVIRGPLQGIRGMLLRKDKRHRLVIGVRLIQQA
                     AAVEIDTGDVVAVG"
     gene            6816..7964
                     /gene="wecB"
                     /locus_tag="VFH05_09680"
     CDS             6816..7964
                     /gene="wecB"
                     /locus_tag="VFH05_09680"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET6678528.1"
                     /translation="MVKFAVIAGARPNFVKVAPLMEALKKAKDVQPILVHTGQHYDHL
                     MSAAFFQDLEMPSPDIYLKVGSGTHAEQTAKILLAFEDYLLNNTVDCVIVVGDVNSTL
                     ACTLASSKCHVPVAHVEAGLRSFDRTMPEEVNRVVTDALSDYLFTPSQDGDENLRREG
                     VPEDRVFQVGNIMIDTLRRYEQAARARLLAARHGLKAKDYTLCTLHRPSNVDNEDTLR
                     GIVAVIRRLAEKQPVVFPVHPRTRARMSAFGLENHMQDTRVLLLDPLGYLDFLSLLVD
                     AGLVLTDSGGIQEETTVLGIPCLTLRESTERPVTVTVGTNRIVGTDPKRIVEAAKSAW
                     AGDWAKGGIPDGWDGHTAERIVDILVRKKPSLAGRKHSSVCELKMPVF"
     gene            7991..9241
                     /locus_tag="VFH05_09685"
     CDS             7991..9241
                     /locus_tag="VFH05_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6678529.1"
                     /translation="MPSYALGNRVHARPVIFQLQTYGLAGVTFLAFFPSLFHYQEYAF
                     FLLLALALALARVERINPHVRTSLDLPLFAFVSWVLLTVPFSIDPAYSFSEWRKFIAH
                     MLVFYWAMFVLRLDADVVPARKALVAIVLGSLALSLFALQDFLIRGGSWRDRLVRAGA
                     PFSDYNWLATYLVLVIPVLIGWLVTQSNLATRAVGLCSLVASVLAQAAAYTRAGWVAH
                     FVQALSFVLLIGRHRLLVWFLSGVILAGSIGLFALSSRGYQQGTLDPWTFSARVKTWQ
                     LGFQQVLEHPIVGIGFGNNIFSQVYTAEIEAGRSKGEVEKVLPGLHNTFAMVLMGSGV
                     PALTFFVWFIIRALRELLAGVRAADVARTRHMVFRLAIALAVIGFVIRNLFDYMFAGS
                     LASLFWIFIAVGLSMKTEQALENN"
     gene            9238..11391
                     /locus_tag="VFH05_09690"
     CDS             9238..11391
                     /locus_tag="VFH05_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HET6678530.1"
                     /translation="MKQVLIKQGQVVVEEVPVPTIGAGTVLVQVRQSCISIGTELSGL
                     RASGEPLWKKAIRYPAKVKKAIDMVAERGLARTISHAQGALTSGQPTGYSAAGVVMAV
                     GPDVHDIAIGDRVACAGAQCAHHAEVINVPRNLTVPIPDGLDFDAASTVTLGAIALQG
                     VRRAAPTLGESFVVLGLGILGQLTWQLLKANGCRVIGTDVSSDRIDLGKRLGMDVGVY
                     PEEGASIDQVVRLTDGIGADGVIITAASPSDAIVSTAFQMCRKKGRVVLVGDVGLQLN
                     RADFYQKELDFLISSSYGPGRYDRRYEEEGLDYPVAFVRWTENRNMAEYLRLLNERKV
                     VLSPLVEATYPISEAPVAYERLKTDHKKPLMVLLTYPQPKEQYSLVRLVSNPLAQPAG
                     KNCIRMAVVGAGGFAKAMHLPNLKALSELFSVHAVMSRSGHNAVDTARQFGAAYSTTD
                     YEAVLKDADIQAVLIATRHHLHATMALEALHAGKHVLLEKPLALTRNELASIKEFFSQ
                     ASGARRPILLTGFNRRFSPAVRRIGELIANRTNPMVLNYRMNAGALPPGHWVHGQEGG
                     GRNIGEGCHIYDLFTALTNSQAEHVHCDTIRPATGHYRRQDNFIATVTFKDGSVATLT
                     YTALGSSEYPKERLEVFVDGKVLFLDDYKRLTIAGATAKGVQSASAEKGQKEELESFG
                     RAIQNGGDWPIPLWQQVQATELSFQVEDQINPGWE"
     gene            11528..>11756
                     /locus_tag="VFH05_09695"
     CDS             11528..>11756
                     /locus_tag="VFH05_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HET6678531.1"
                     /translation="MCGIVGTLVFDGASFRVTEPYISRMRDVMRHRGPDGAGVWIATD
                     ARVGLAHRRLSIIDLSEAATQPMSNEDGTLWI"
BASE COUNT         2468 a         3063 c         3509 g         2716 t
ORIGIN      
        1 gaatatcgta gactgcctcg catcggtcgc gtgggccggc gagcggatcg tgatagatgc
       61 ggaaagcagc gaccgcacag ccgaactggc gcgtgccgcc ggcgcaaggg tgatcgtgcg
      121 gccatggccg ggttttggcg cccaaaaaaa ctacggcatg gatcaggtcg ggaccgaatg
      181 gattctgatt ttggatgctg atgagcgggt gccgccggct ttgcgcgatg aaatcctggg
      241 acggatagga tcctggaaac caggggaacc tgtggcgtat gaaattccgc gcaggaattt
      301 cttttatgga gcatgggtgc gtcatgccgg tgtctatccc gattaccaga tccgcctctt
      361 ccggcgaggg tacgccaggt ataacgacgt gcccgtgcat gaaaacttga tggtccaagg
      421 cgtgattgga aaactggcca atccgttgga ccacttcacc gagcgcagta tcagggacca
      481 ttttcgaaag tttggccact atacgaccct ggcggcgcaa gagaaagccg gcagggttcg
      541 ccatgtgggg gcttcggacc tccttttccg gccacttgtc gtctttgtaa aaagttatgt
      601 actcaagcgg ggctatctcg acgggatacg tggtctgatc gtgtccgtct ttgccggcat
      661 gtacaccttc gtcaagtatg ccaagttgtg ggacatgacc cgatcctccg tcggcgttcg
      721 ggatgacagc tagtggcccg accaatcctg tatatccatg gggtcgccgc cgtgggcggg
      781 gccgaacgtg atctgctggc catactccgg acactcgcca ggctcgactg ggagccgcat
      841 gtcgcctgtc cctccgaagg gccacttgcg aaactcatga ttcaagccgg cgtcgtgctt
      901 catccgatgg ttttcccccc gtggcgtaaa tggttttcgc cgttgatccg gtggttggca
      961 atcaaaaaac ttcggacctt gcttgagcag ctcaaacctg cgctgattca cgtgaatgat
     1021 atctggtggg tgcctcagac ccttgcagcg gtcggcggcc gtcccggtct tcgaataccg
     1081 gtcgtggcgc acgtgcgtca agaaattgag ccggaaaaag tcaggcgata ccgcctccac
     1141 gagctcgacg gcgtgatcgc gatttcccgg cacgtcgaac gggcgttgat cgccggcggc
     1201 gtcgaccagg ggactgtccg gaccatttat agcggagtag aactgctgag aagcgatgct
     1261 ggaatgtcgg atcgaacggc aatttgccgc tcactcgctg tgtcggacga agccgtcttg
     1321 ttggggacgg tggcgcatct ctttccgcgg aaggggtatg acgtcatgct caaggcattg
     1381 cccggtatca tacggactgc gccggcagtg cattatgtga tcatcggcac cggcgacggg
     1441 ggttatgaac agaggttgcg agcgttgtcg gccgaactcg ggattgctga tcacgtccat
     1501 ttcgtcggat ttcaggagga cgtgaagccg ttcctgggag cgttgtcctg ctatgttcat
     1561 ccggcgcgta tggagggctt tggcattgca gtcgtggaag cgctcgcagc gggaaaagcc
     1621 gtggtcgcga cgaatgtggg cgggcttccc gaggtcgtcg aggagggaga gaccggactt
     1681 ttggtcgccc ccgatgatcc tggcgcattg actgctgcgg tgctgtctct actgcgtgat
     1741 gacggccgga gaaggaccat gggcgagcga gccgtgaaat gggtccacga gcgattcgat
     1801 ctgcgcgagt cggtggcagc gatggagcag ttctatcagg aggtgctctc agcgcacccg
     1861 aaccgaacat gaggatcggc atcgatgcca gtccagtcgc aggcgatcgc ggaggggtgg
     1921 gttggcatac ctactatttg ctgcgagcca tgttggccgc tccttcggag gcgcgcttcg
     1981 tggcatacct gagaccgggc atgactccac catccgaggc atctgagtgg gctggcgtcg
     2041 atcgtcttga gtgggtgacg gtttcgaagt gggccatgac aagacgagga acagccgatc
     2101 ggttggatct atatcatggg acgaattttc gaatgcatac ggtgggacgg tttggcggca
     2161 tcgtcacgat tcatgatctg tggctcgaac gatttccgga gtattcgtca aaattgctcg
     2221 gccagagatt gtcgtcatac aaaacgaaac gcatcgcccg gcgcgcgaaa aaagtcgtca
     2281 cggtttccca attttcggcg cgggaattga tgtcggtgta cggtctgtgt gaacaacaca
     2341 tcgccgtcat cccaaatggc gtctccgaag agttcagccc gcgacaggac gctgcggaga
     2401 tgagcatctt gaagcagcgc atggggttga agactgatcg gtttatcctc tttgtcggag
     2461 gcgcggatcc acgcaaaaac catcgaaagg tcctcgaggc tgcgtcactc attcgaggac
     2521 acctggatgg tcgaacggtc cttcttgtcg gttcaccgac gcatccattc gggtcgtatg
     2581 aacggacggc tgcttcatac ggattggctg cccacgtcct gtgtccgggg cgattatcga
     2641 gagaggatct gcggttgctc tactcctatg ccgatctctt tgtgtttcca tccctgtatg
     2701 aggggttcgg catgccggtg ttagaggcca tggcgtgcgg tgcgcccgtc atcacatcca
     2761 aggacacggc tctgggcgag gtggccggcg acgccgcagt ggtcatcgat ccgcacgatg
     2821 cacgggcatt ggccgactcc atgatccggg tattggagaa tgacagtttc cgcgtctcac
     2881 tgagagagaa gggtttcctt cgggtcaagg actaccgctg gtcccatgta gcggcacgga
     2941 ccttgtcgtt atactcgtcg ctctgccggt agaattggtc gcatggcacc gcacaacatt
     3001 ctcaccatca aattgcggta tctcggtgat gtgctgctgg cgacgccgac atggcaggcc
     3061 ctaaaggcgg cctatccgag agccaagttg accgcaatcg tgaatcgggg tacagaggat
     3121 atcctactgt caaatcctca tgtcgatgaa gtcctttcac tggagcgcgg ttcactggtg
     3181 cagcagtacc ggttcattgc cagcatccga cgacgtggct tcgacacggt cgtggatttg
     3241 accgatggcg accgggcagc ccttctcacg tggatgagcg gtgcacgcgt gcgcatcggc
     3301 tttaatgcag agcaccgctg gaccggacgc tgttacacgc gtgtcatcac cagcgatgca
     3361 ggcgctcatc gaatcgaacg cgatttgtca gcccttgccc cgctggctat tcccgtcatc
     3421 gaccgcatcc cgagaatgtg gttaacggag gaggatgacg cagcggcaga cgatcttctg
     3481 cgacgtgtcg gcgtgcaatc ggacaggcgg tgggtagtga tccaaccggg cgctcggtat
     3541 tggtttaaag cctggccgcc agaacgattt gccgagctcg ccgaccgggt ggccgaccgg
     3601 tacggatgtc aggtgttggt ggccggcacc ccacaagagg cggcgctcgc gcagacggtc
     3661 gtggcccagg cgaagcaccg gttgatctcc attgccggtc tgtctcgtgt caggacgctc
     3721 gcggcgttgc tgaaacggtc ggcgttgttc gtcggcaatg acacgggagc catgcatatc
     3781 gccgcagcag tgggaacgcc ggtggtcgga ctgttcggtc cgtcgaatcc tctggaatgg
     3841 ggaccacgtg gcggccctgc ggaagtgatt tacaaaggtc ttgattgccg ggagtgtttt
     3901 catccgacct gccggcgggg ggagcaaaat tgcatgaagc tcattagcgt gagcgaagtg
     3961 atgtcggccg cagagcggct actggcacac ggactaagct gaaaggcata ggccctgcgg
     4021 gaggttattg gtgggcgcga ttcttgacat cggttgtgga gcgaacaaac acgagggagc
     4081 acttggtatc gattgccgga aggtgaccgg ggttgctgtc atctgcgatt ttgagcaggg
     4141 actgccgttc cgagagggga gcgtggcagt cgtatatctc catcacattg tcgagcatat
     4201 gcatgacttg attgccttca tggttgaact gtaccgggtg tgtgagccgg gcgccaagtt
     4261 gtttgtgcgg acgccgtact acgcctcccg ggaagcgttc gtcgatccaa cgcacgtccg
     4321 gtttatcacc gaagcgacct tcgaatattt cgattaccca aattattacg gtctcaatac
     4381 gaacattaag atacgttcca ttgattataa gatgcggaag tccttgaggt tcttgccacg
     4441 ctactttcaa aaacgctgtc ggcagttttt gtggaacgcc tgcattgaaa tggatctcct
     4501 gcttgaagtc gtgaagccct tacgcccacg aagcgagtga gacgtgcgat gtgcaaggtg
     4561 actgtcgtca taccggtcta caacgggggc cgatatctcc gcgaatcgat agactcggtc
     4621 ttggctcaaa catttcatga ttatgaaatt gtctgcgtgg acgatggctc gacagacgat
     4681 tcgtcagtcc tgctccaaga atacggccag cgtcttcgag tggtgcgtca agagaactca
     4741 ggccagagcg cagcacgaaa tgtcggtgtg aagctggcgc gtggtgaata catcgctttc
     4801 cttgatcagg atgacgtgtg gtacccatcc aaagtccaaa atcaggtggc tgtgctggac
     4861 gcggagcccg atgtcgtctt ggttcattgt aactttgacc ggatagatga gcgaggccgg
     4921 atgcttgaac gcggagcggg gatgatcgaa cggacgtcag cgcttggttc ttcgatggga
     4981 caactgatcg gcgaagcact catttttcca tcggccatga tgatacgcaa agagggttat
     5041 gaacgaatcg gcgggtttca tcacgccctg caaggattcg aagacttcga tctcattgcc
     5101 agacttaaag cccaaggacg attcgtcatg gtggaggaac caggcatggc ctacagaatg
     5161 catggaaagg ggttcacccg aacaggaggg attcacgtca tccgtagccg ggagacgttt
     5221 cttcgccgca tgcaaacgct gtatcggggg catccgagca aacaggccat cgtcaaacac
     5281 atgctggcag attgttattc cgattggggg atgcatgatg tccgaattgg aaaccgtcag
     5341 gaagcacggg caaaactcct tcaatcgatc cggtgtaacc cgttcaaatt tcggacctac
     5401 acccgattgc tccgcgctat tcttccttcg agtcgtcttg tgtagccgct cttccttgac
     5461 taggcaaacc agtttagttc atattgtgaa cgcttcgccg aattacctgc acatttcatg
     5521 gatttacaag gccaacgcga tctcttactg ctgagcgaac tcgatcgaaa cgggggagcg
     5581 acgcagcgca cccttgcgag caaacttggg gtggccctgg gtttgaccaa tctgtatctg
     5641 aagcgcttgg tccgcaaggg ccacatcaag attatgacca ttccgcgcaa tcgcatccgg
     5701 tatttgctca cttcccaagg attcgcagag aagtcccgcc tgacctatca atacatgcag
     5761 tattcgctgt cttattaccg cgacatgcgt ggacgtctca acgacatgct gtcgacgttg
     5821 gaatggaccc acggacagcg tgtggcgatc tgcggcacag gggagctggc tgagttggcg
     5881 tatctggcgc ttcgggaaat gaatgtgaat tgcgtcgggt ttgtggacgg caatacacgg
     5941 gagagcttct tgtcgtgtcc cgtgtcttcg ccggatggga tcgccggttg gcaattagat
     6001 cgagtgctca ttgccgattt cgaccatgcc gagacctacg aagagcaatt ggtacggtcc
     6061 ggcatccctc gcgagaaact gctgagactg ggcctgcgcc actagggcga taatcccggc
     6121 agtttttgta gaggcactgt aagtgaaagg gcgaagctat gtccgttgag ccggcaacga
     6181 atcagacgaa cagttcccat tggtacgcgc tgcggacgcg atcgcgtcat gaaaaaatgg
     6241 tgcgggatca gctgatgcaa caaggcatcg agcatctgtt gccgaccgtc aagcgcctca
     6301 gtcaatggaa ggatcgcaag aaagaagtcg aagtgccgtt gttctccgga tattgtttcg
     6361 tccgattcgg tccggaccag aagctgccaa ttctgaaaac ggtcggcgtg gtcgacattg
     6421 tgggcagtgg gcaacgccct gaaccgattc ctgatgagga aatcatcgcc atccaaacgt
     6481 tgatggccag tgtcctgccg tacgatccgc atccttatct gcaggaaggc atgctcgtcg
     6541 aagtcatcag agggccgctt caaggtattc gagggatgct gctccgcaaa gataagcgcc
     6601 atcgattggt gatcggtgtg cggctcatac aacaggcggc cgccgtcgag atcgatacgg
     6661 gcgatgtcgt ggctgttggc taaccgcgca catgtcatca attgtttcct gttttcgggc
     6721 tccttcttgt cttaccaaca actgcttccg atcacgagac ctactcatcg gcgttattga
     6781 tctcatcctc ccagtcgcgg taaggataaa acactatggt taagtttgcg gttatcgcag
     6841 gcgcccgccc aaattttgtg aaggttgctc cgctgatgga ggccctcaaa aaggccaagg
     6901 acgttcaacc cattctcgtg catacaggac agcactatga ccacctcatg tctgcagcat
     6961 tcttccaaga cttagaaatg ccgtctccgg atatctattt aaaggtcggt tcggggaccc
     7021 atgctgagca gaccgccaag atcctattgg ccttcgaaga ctatctcctg aataataccg
     7081 tcgattgcgt gatcgtcgtg ggagatgtga attcgactct ggcctgtacc cttgcttcgt
     7141 caaagtgcca tgttccggtg gctcatgtgg aggcgggact acgaagtttc gatcgaacga
     7201 tgccggaaga agtgaatcga gtcgtgaccg atgcactgtc ggattacctg tttacgcctt
     7261 ctcaagacgg tgacgaaaac ctgagacgtg aaggcgttcc agaggaccgc gtgtttcagg
     7321 tgggtaatat catgatcgat acactccgtc gatatgaaca ggcagcccga gcacgcctcc
     7381 tcgcggctcg acacggcctc aaggcaaagg actatacgct gtgtacattg catcgcccaa
     7441 gcaatgtgga caatgaggac accttgcggg gcatcgttgc ggtcattcga cgattagcag
     7501 aaaagcaacc cgtggtgttt cctgttcatc cgcgtactcg cgcgagaatg agtgcgtttg
     7561 gattggagaa tcacatgcaa gacacgcggg tgcttctact cgatcctttg gggtacctcg
     7621 attttctcag cttgctagtt gatgcaggtt tggtcctcac tgattctgga ggcattcagg
     7681 aggaaacgac cgtcttaggg atcccgtgcc tgacattgcg agagagcacc gagcgccctg
     7741 tcacagtgac cgttggaacc aaccgtatcg tagggactga tccaaaaagg attgtggagg
     7801 cggcgaagag cgcttgggca ggtgactggg cgaaaggcgg tattccggac ggctgggacg
     7861 gtcatactgc tgagagaata gtagacatat tagtccggaa aaagccgagc ttagcagggc
     7921 gcaagcattc ttcggtctgt gaactgaaga tgcctgtctt ctgaccgttt ctttcgaaag
     7981 tcgggtcaac atgcccagct atgccttggg aaatcgtgtt catgcaagac ccgttatctt
     8041 ccagcttcaa acctacgggc tggcaggagt cacatttctc gcgttctttc ccagtctatt
     8101 ccattatcaa gaatatgcct tcttcttgtt actcgctctc gcactggcct tggcccgcgt
     8161 ggagaggatc aacccgcatg ttcgaacgag cctggacctg ccgttattcg catttgtcag
     8221 ttgggtgttg ctgacggttc ccttttcgat tgatccagcc tacagttttt ctgaatggcg
     8281 aaaattcatt gcgcacatgc tggtctttta ctgggccatg ttcgtcttgc gtttagacgc
     8341 ggatgtcgtg ccagcacgca aagccttggt ggcaatcgtg ttgggcagcc ttgcgttgtc
     8401 attgtttgcg ctacaggatt tcctcatccg tggaggttca tggagagatc ggttagttcg
     8461 tgctggcgcg cccttttccg actacaattg gttggccacc tatctcgtgc ttgtcattcc
     8521 tgtgttgatt ggatggctcg tgacccagtc gaatctggca actcgagcgg taggactgtg
     8581 tagtctggtc gcatcggtgc ttgcgcaggc tgcggcgtac acgcgagccg gctgggttgc
     8641 ccatttcgtc caggcattga gctttgtgct tctcataggc cgacaccgat tgctggtgtg
     8701 gtttctcagt ggcgtcatcc tggcgggaag cattggattg ttcgcgttgt cttctcgcgg
     8761 ctatcagcag gggactctcg atccgtggac gttcagtgca cgtgtcaaaa catggcagct
     8821 tgggtttcag caagtgctcg aacaccccat tgttggcatc gggtttggca acaacatctt
     8881 ttcccaagtg tacacggctg aaattgaggc gggcaggagc aaaggagagg tagaaaaagt
     8941 tcttccaggc ttacacaaca cctttgcaat ggttctcatg ggaagtggtg tgcctgcgtt
     9001 gacgtttttc gtctggttca tcatccgtgc tttgcgggag ctgctcgccg gtgtgcgagc
     9061 ggctgatgtt gcgcgaacaa gacacatggt attccgcttg gccattgccc ttgccgtcat
     9121 tggatttgtc atacgaaacc tttttgatta catgtttgcc ggaagcctcg cctcattgtt
     9181 ttggattttc atcgctgtag gactttccat gaaaacagag caagcgttgg agaacaattg
     9241 aagcaagtcc tcattaagca agggcaagtt gtcgtcgaag aagtaccggt ccctaccatc
     9301 ggggcgggca ccgtgcttgt acaagtgcgc caatcgtgta tttccatcgg aacggagctc
     9361 agcggtctta gagcaagcgg cgagccgttg tggaaaaagg cgatccgata ccccgcgaaa
     9421 gtgaaaaaag ccatagatat ggtggccgaa cgaggccttg ctcgcacgat cagccatgcg
     9481 caaggtgctc tcacttccgg tcaaccgacc ggctattccg ctgccggcgt cgtcatggct
     9541 gtggggcctg acgttcatga catcgccatc ggggatcgtg tggcctgcgc tggagcccaa
     9601 tgtgctcatc atgccgaggt cattaatgtt ccccgaaatc tgacggttcc gattcctgat
     9661 ggcctcgatt tcgatgccgc cagtactgtg actctaggtg cgattgcgct acaaggggtc
     9721 cggcgcgccg ctcccaccct tggagagagc tttgtggtcc ttggattggg tattctgggc
     9781 cagttgacgt ggcagttgct gaaggccaat ggctgtcgtg tcatcgggac ggatgtgtcg
     9841 tcggaccgga tcgatctcgg aaagcggctg ggtatggatg tcggcgtcta tccagaagag
     9901 ggagcgagta tcgaccaagt agtccgtctc acggacggga ttggtgcgga tggtgtgatc
     9961 atcaccgcag ccagtccttc cgatgccata gtctccacag cgtttcaaat gtgccgaaaa
    10021 aaagggcgtg tcgtgctcgt gggcgatgtc ggacttcagc tcaaccgggc tgatttctat
    10081 cagaaggagc tggatttctt gatttcgagt tcttacggtc cgggtcgtta cgatcgccgg
    10141 tacgaggaag aggggctcga ctatccagtc gcgttcgtgc gctggacgga aaaccggaac
    10201 atggcagagt atctgcgact gttgaacgaa cggaaagtcg tactgtctcc actagttgag
    10261 gcgacctatc cgatcagcga agccccggtt gcgtacgagc ggctaaaaac cgatcacaag
    10321 aagccgctga tggtcttact aacttatcct cagccgaaag aacagtattc acttgtccgt
    10381 cttgtgagta atccgctcgc tcagcctgcc ggcaagaatt gcatccgcat ggccgtggta
    10441 ggcgccggcg gatttgcgaa ggcgatgcac ctgccgaact tgaaagccct atcagaactc
    10501 ttcagcgtgc acgccgtcat gagtcgctcc ggtcataacg cggttgacac agctcgacaa
    10561 ttcggcgccg cctattcgac tactgactat gaggcagtgc tgaaagacgc tgacattcaa
    10621 gctgtgctta ttgcgacgcg ccaccatctc cacgcaacaa tggcgcttga ggcactgcat
    10681 gccggcaagc atgtgttact tgaaaagcct ctggcattga ccaggaatga actcgcttcc
    10741 atcaaagaat tcttttcgca ggcgagtggg gcgaggcggc ccattttact gacagggttc
    10801 aaccgccgtt tttcgccggc agtcaggcgc atcggtgagc tcatcgccaa tcgaaccaac
    10861 ccgatggtgc tcaactaccg gatgaacgcg ggcgctcttc cccccggtca ctgggtgcac
    10921 ggccaagaag gtggtggtcg caacatcggc gaagggtgcc acatctatga tctgttcacc
    10981 gcgctgacga acagtcaagc ggagcatgtc cactgtgaca ccattcgccc ggctacaggc
    11041 cattaccgcc gccaggataa tttcatcgcg accgtgacat ttaaagacgg ttctgtggcg
    11101 accctgacct acaccgcgct cggaagttca gagtacccca aagaacgttt ggaagtgttc
    11161 gtcgacggca aagtcttgtt tttggacgac tacaaacgtc tcacgattgc cggtgccacg
    11221 gcaaagggcg tccagtcggc gtcagcggaa aagggacaaa aggaagaatt ggaatccttc
    11281 ggacgagcaa ttcaaaatgg cggcgactgg ccgattccgc tgtggcagca ggtgcaagcg
    11341 accgagttaa gttttcaggt ggaggatcag atcaatccag gctgggagta agagtgttga
    11401 ctatcgcccc gtcagactcc tgcaaagtac ccaccgctgt cctccaaaca tgacgatcct
    11461 cccgcgcccg agttcacacg gtacatgcgc tcggttggca gcagctgagg taggtaggaa
    11521 catttaaatg tgcgggatag tcggcacatt ggtatttgac ggggcttcat tccgagtcac
    11581 tgagccgtac atctccagaa tgcgggatgt gatgcgccat cgtggccccg acggggctgg
    11641 cgtctggatt gctacagatg cacgagttgg attggcccac cggcgtctgt ccatcattga
    11701 tctttcggaa gcagcgacac agccgatgtc caacgaagat ggcacgctgt ggatct
//
LOCUS       DASSCQ010000144        18767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1671818, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000144 DASSCQ010000000
VERSION     DASSCQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 18767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18767
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671818"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFH05_09700"
     CDS             complement(<1..249)
                     /locus_tag="VFH05_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678532.1"
                     /translation="MQGTDEQAAGRKRTVHPLHLPKVHLPTYDETGAQAADWPLRRRR
                     VTIGPIAYLAFCLLLWASCEPIATQTYQVRQRAFTYTPS"
     gene            481..924
                     /locus_tag="VFH05_09705"
     CDS             481..924
                     /locus_tag="VFH05_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HET6678533.1"
                     /translation="MSVPVGSEETILPSSAPAASPSVPVQDMVGEGKAWGLCTAVDLQ
                     DCHPDLIRDAEHIRRYVAELCDLIRMQRFGECQVVDFGSGRVAGYSMVQLISTSLISG
                     HFANDTNSAYLDIFSCKGYDPSVVEEFSKEFFRARRSTATVTLRY"
     gene            985..1326
                     /locus_tag="VFH05_09710"
     CDS             985..1326
                     /locus_tag="VFH05_09710"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET6678534.1"
                     /translation="MTAPTIKDFFKHLPGKFDAEAADDLEAVYQFDLSGPQGGQYVIT
                     IRKGACQVREGRDDDPHVTLSMASEDCIKVLNGQLSGPAAAMSGRIKITGDMGLAMQL
                     KALFPTIGEQL"
     gene            complement(1377..4568)
                     /locus_tag="VFH05_09715"
     CDS             complement(1377..4568)
                     /locus_tag="VFH05_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6678535.1"
                     /translation="MWLTLLALRNRIGILMLSLAMVVLGATSLNRLPVDLFPNIQVPV
                     AFVGVIYKGAPPLDIEQSVVYPIEKAVSSASNVEHVESFAKQGIGAVQIWFNWGADIN
                     VGQMEVMQRITQILNSLPPGILQPFIVKFDVSNIPVSFVTVSSDALDERALYDLAFNT
                     IAPQIEQIANVAAATVEGGKIRQININLDPALLNARGLSILDVVKSVKAANLILPSGD
                     IKAGNLDYNVFTNNQFRTVEPIQDVIVKVNQQGNPVRVRDLGTVTDSSDIQTNIVRTD
                     GKRAVYLRVNKQPIANTVEVVDALRAALPKMIGIPPGVKLGISFDQSVYIRQSIGNLV
                     EQALHGSLLAAAVILVFLRNVTSTLIISVAIPLSIMVTFIVLYFTGQTLNVFTLGGLA
                     LGIGRLVDDSIVELENIQRHLNTNQRRWDAILEAAREVAMPIFASTVTTVVVFLPIFF
                     VVGIARLLLIPLTLTIAIALFTSFFVSRTVTPALCFKFLKPEQESHRSMPAWFVSLMT
                     WSRERYESLDRGFEESLRWVLTHRRLFIVGILVIFGASLALVPRIGTEFLPVSDESQF
                     RIVLRAPVGQRVEKTEQQVAEVERVLRTTIPAHELETIVSSTGVLAQGRSSLFNPNTG
                     PHTSVISVYLSSPEHRSRNQVQIMNDVRPKIVKLFPGVAMFFDPGGLVKRVTSFGSQK
                     AVDVEIYGYDFENARTVIGQVEGVMHKVDGLADIEVSREENYPEVNVVVDREKAALLG
                     ISETDIANAVLFSLNGNGQTDPIIYTDPQNGNEYYISAWLAEEHRKDLTDIENIILTA
                     RNGEPVLLKNVATLKLNAGPVKIDRKFFQRVIHITANPLNRDLGAIAEDLETAFAKLQ
                     LPNGFSIRLAGQIQQQRETFVGLMFATVLALILVYMVMAAQFKSLVDPFIIMFSVPMG
                     FPGVILVLFLTDTTLSTTSMMGIIMMLGIVVSNGVLLVDYTNVLRRKGLALQEAVVLA
                     ARTRLRPILMTSLATVFGLLPMAIGWGTGGETNAPLARAVVGGLSVSTLLTLFLVPTM
                     YLILEERFPRRATQEPTVFEELPSEAPLVPANR"
     gene            complement(4644..5819)
                     /locus_tag="VFH05_09720"
     CDS             complement(4644..5819)
                     /locus_tag="VFH05_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6678536.1"
                     /translation="MNPVRQHPLVTLGIIIFFAVTALVVFRLSSGAKIDQKKSRVITV
                     GTVTPLKQDLEIRLGYTADITPNQVVNVFSRVDGYISKLHVDKGDFVKANQLLLEIDH
                     QDYLHNVNQAKANLAAARAKVTQQEASVRNAKLTLDRMQALIRDQFVSQQDVDTAEVN
                     SDAAIAALESLRAQVKQMEVALAQAETNLTYSYIRAPFSGYVAERNLDLGAYVTGAAT
                     SNSTTSRGIVSLHDISTVRTLIEVVEKDVPLVRIGQKAEIRAEAYPDRVFEGTVTRVV
                     QALNRATRTMTVEVDLPNNDRLLKGGMFARVEVLVGKHQNALQIPIDAVSRLEDAQYV
                     YIVRDGKAERVSVEIGVRDENRVEITKGLVGSEQVIVSGKDLVHDGMPVQTEPLTRS"
     gene            complement(5816..6430)
                     /locus_tag="VFH05_09725"
     CDS             complement(5816..6430)
                     /locus_tag="VFH05_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6678537.1"
                     /translation="MATRSTSQQRQAGLIAAAASLFAAKGFNGTTTKEIAKAAGVSEA
                     LVFKHFPTKRALYGAILAEKVTLDELLGAVEESARKRDDRRVFTLIAGFRIKPSVDST
                     LLRLLLFSALEGHELSDMFFGKHHRVLYDHLATYIKTRIDEGAFRQVDPLLAARAFIG
                     MVVHHRLLHEIFGVPMHQTHDATVRTYVDLFLTGIMKARRSRPL"
     gene            complement(6530..6910)
                     /locus_tag="VFH05_09730"
     CDS             complement(6530..6910)
                     /locus_tag="VFH05_09730"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678538.1"
                     /translation="MKQDRARLSKTLHRTHGVCDRRNEPRVPVDVRLMYSAQDEENVL
                     ITGDGSVVDLSNSGLRIHGNVAVRSGLEVTLFLYLPDGRDPLLVMSAKVTWSRGHAFG
                     VEILKMNVRERNRLRYFLGTNLPR"
     gene            complement(6925..7320)
                     /locus_tag="VFH05_09735"
     CDS             complement(6925..7320)
                     /locus_tag="VFH05_09735"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678539.1"
                     /translation="MKHTSTDRSRRTADKPPDDNRRGTERVPTQFSLMYSGMYDGRML
                     IGNGVVRNISEAGIGIHGNQVVKLGMDLSLFIDLPGVEEPVCIAESRVSWVSGRRFGV
                     EMIAPKLEVQNELRFYVWNNLFRKKANKS"
     gene            complement(7363..7782)
                     /locus_tag="VFH05_09740"
     CDS             complement(7363..7782)
                     /locus_tag="VFH05_09740"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678540.1"
                     /translation="MKCSLLEQNLSAPRAHAPLDRRYAERALIHHEVTYTGTEGARFT
                     TSIGSLKDLSKTGCKILGTTLPVIGGHVTVTLYLNDGQAPLCLTDATVSWIKGHVFAV
                     RFPKLTDDERKRIQGIIWKHVRLPEANHHRTAFRIVP"
     gene            complement(7933..9612)
                     /locus_tag="VFH05_09745"
     CDS             complement(7933..9612)
                     /locus_tag="VFH05_09745"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET6678541.1"
                     /translation="MHNATKLRAALKKPGALKVVGAHDALSARLIERAGFDGVWASGF
                     AISASLKCIPDASFITSSEQLEVERNIAEAVNIPVIADCDTGYGNALNVMRTVNDRER
                     AGVAAICIEDNVYPKRCSFYAGVRRELIPIDEHCGKIKAAKAAQTVPDFMVIARTEAL
                     IAGWGQEEALKRAEAYAEAGADAVLIHSKSKTFDELKTLYRAWSGRVPLVVVPTIFDQ
                     TTAQEMEQAGAKIIIYANQPVRAAIRAMRETLDLIRTDTRPGAANDRIVTLPEVYDIV
                     GVPQMEQDEKAFLPIGGEKITAIIPAAGFEKQLLPLIEDKPKCLLDIKGKTILERAVA
                     ALNEANIKEIAVIRGYKKEAIALPNIRYYDNDRYEDTGELFSIFCAEPEMKGRTIVLY
                     GDIIFDTTILEKLLKSSADFALVIDLAWRDHVDRGGAPVHLNPDLVTLADPPGSSYLS
                     RFVMGEDEHRIVKIGQHLPLDQVHGEFIGMAMFSEKGTQAMRECYRSSQERYKSAAFH
                     EARSVSKASFTDLVQELIDEGHRIDAVPVFKGWMEVDSFEEYQKAWAKIRQ"
     gene            complement(9681..10229)
                     /locus_tag="VFH05_09750"
     CDS             complement(9681..10229)
                     /locus_tag="VFH05_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678542.1"
                     /translation="MFIRDFLLRQVFLAMLLSGNLLVVTSTLAIEMKDDPKGFEGIPW
                     GATLSESDTFGKVEDAGRLKTYELKGEPPTFGPAKVESMRFTTVDDRFARVTVRYRGR
                     EAHDQLLGFLQSRYGPLDRTPGQFAGGAVKIFAWQGLETVVSLRYETRSDRGIIFFEN
                     PALAARIEGAMAPDQDLGGATY"
     gene            complement(10233..10739)
                     /locus_tag="VFH05_09755"
     CDS             complement(10233..10739)
                     /locus_tag="VFH05_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678543.1"
                     /translation="MRLLFLLVSGMIAFSPGPAGAVSMTNDPKGFHNIVWGTALNTRP
                     DLELTRQGRNIIEYQFKDTPPSFATIPVESLRLSTIDDQFARVTIRYRGAETHKKILS
                     YLESEFGRTEHLPGQMLRGLNQQYNWRGSESEINLTYQSSTERGYIFLDSRTLAPRFN
                     DLLADTAE"
     gene            10861..11358
                     /locus_tag="VFH05_09760"
     CDS             10861..11358
                     /locus_tag="VFH05_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET6678544.1"
                     /translation="MIESDAFVQALQDMGVNFFTGVPDSMLGGIIAELMDRRLYTPAV
                     REDEAVAMAAGAYMGGKIPAVLMQNSGLGTSLNTLISLNLIYHQPCILIVSWRGQGGK
                     DAPEHLVMGEVMPQLLDTMKIPHRTLTEAGAIEDFKWIAETFMKQRIPVALFITKGVV
                     KGLHP"
     gene            11355..11933
                     /locus_tag="VFH05_09765"
     CDS             11355..11933
                     /locus_tag="VFH05_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6678545.1"
                     /translation="MRPEQGTLISRAQAMAALLELLTDQPVIICNGFPSREAQKLVDR
                     PTHFYMIGSMGSAPAIALGVALAKPHKQVITFDGDGNVLMGMGTLATVAALKPKNFIH
                     VVFDNEVYGTTGNQPTISNVVPLEKVAKAAGYTNVERVLDLDDLVYEFKDMLKKDGPS
                     MLLIKVNEFAEDAGRVVLDPPDITKRFMKAIE"
     gene            12048..13133
                     /locus_tag="VFH05_09770"
     CDS             12048..13133
                     /locus_tag="VFH05_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET6678546.1"
                     /translation="MMLLNPGPVNVSERVRQALTRPDICHRESDFAELQQGIQAKLLK
                     AFVPGAEAEYAAILITGSGTAAVESALMSSLPHGKRALIINNGVYGERMSQMVGLHRL
                     GVSELKHEWTERPDPERLRLALRQHPEVHAVGMVHHETTTGLLNPGKAIAEVVDGLNR
                     VFILDAVSALAGESIDIAGSKIYMVAGTAGKCIQGFPGVSFVLVRKGFLERMRAYPKR
                     SLYLHLTHYVDDQGRATVPFTPAVQLYYAFDEALSELLEEGVAKRIQRYKKMAQLIRE
                     RMAKIGIKPVLQPDRQSNSITAYYLPEGVAYPTLHDRLRAMGYVIYAGQGNLENKIFR
                     IANMGAVTEAQFIAFLDAFEQIVGGHP"
     gene            13283..14002
                     /locus_tag="VFH05_09775"
     CDS             13283..14002
                     /locus_tag="VFH05_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HET6678547.1"
                     /translation="MKAVILAAGVGKRLWPVTQHRPKCLIELGGQTLLSRYLSSLASE
                     GIRRADIVVGYKQEMIREAANSESFGVQVNFLVNEQFHRGSISSLWVARTALDDDVVI
                     MDADVLFHREILRRLVHSPYPNALLMDDSVKQTGEECMVVAEGGRVIALTKKLPLRYD
                     HAGEGVGFLKVRHMDTPHVVASLRTFVDREAWQMEYEDALIEFFREVKVGYEVIGGLP
                     WTEIDFPVDVEKAERDVLPKL"
     gene            14065..15450
                     /locus_tag="VFH05_09780"
     CDS             14065..15450
                     /locus_tag="VFH05_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6678548.1"
                     /translation="MTERALDKETELQGLSTAVLMPSIDLLGAPERKAVDPLTPIVGV
                     GLFQRTVLTLQRAGIRQLIVVAGQEEDRLKQALLKGPRVSIPVRWMPIREFPLDDPRT
                     WESLAAEVRGFCLLSGVATVFSRALIEQLRRDVQEGLAIIVTQREHRPDVQKRWLHEG
                     WSGMSDGLADVLVVPTTELSAAAQVAGELETVPISRWLQRAMVDGRVRVTSIDADPSC
                     WYQTVVTSEDLQRAEHRLYTSLKSEFEGFVDRYFNRKLSRWCTRVFIAMGWSPNAITL
                     TATVIGLVSAAAFAVGTYAAGVAAALLFQLAAVIDCSDGEVARLTFTDSPFGAWLDIT
                     LDNLVHMAIFGGIAIGVYSNASGSEYAWVGLALGAAAILGNALSFALVNKAQKITTGR
                     KWDSPKHAALSEFMLKNVASRDFSVLVLLFAVFGKLEWFLWIAAIGSMVFAAVMLWIV
                     RPSGLFLRPVR"
     gene            15571..16593
                     /locus_tag="VFH05_09785"
     CDS             15571..16593
                     /locus_tag="VFH05_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6678549.1"
                     /translation="MAIFTRLTVFRYLLLAAGLLTLSFLVWHIGPVHIYEAVARFGPG
                     ALVVMLIPSVVMYVLEAYGWKIVLGSTAERISFGKILSIRTAGEVVNMTTPTAYIGGE
                     PLKAYLLQRHHIPMVEGLASVVIAKTIMTIAQVLFILIGIALAFWTLGAAGSSLQMTA
                     AALLSAALLSCGIAAFVLVQRRGLFTWLLETFRRVGLRMTFLEVRAEKLRSLDRTILG
                     FYAHHQSAFYASTGLYLLGWLAEAVEVFVIISFLGGPASLLSATAIGALSVLIKGGTF
                     FIPGSLGAQDGGNLLLLRAFGYSDLTGMTFALLRRFRELVWIIIGLACLAILGKKSPG
                     SQRPKS"
     gene            complement(16590..16955)
                     /locus_tag="VFH05_09790"
     CDS             complement(16590..16955)
                     /locus_tag="VFH05_09790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678550.1"
                     /translation="MKESRPYHRGLYRLPLSYPVMYCVAATVGEGTISNLSAVGCSLK
                     TDEPLQENQQVALRLLLPDQSESLPIDAAEVKWVKGTHAGIEFVHVDREANLRLHSFV
                     WDKMIERIHFLQQQRSGSS"
     gene            complement(17016..18002)
                     /locus_tag="VFH05_09795"
     CDS             complement(17016..18002)
                     /locus_tag="VFH05_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6678551.1"
                     /translation="MPILSMLGVVALLSTITPAMKYVLEHSGLTFLSIANGRIVIGFL
                     FLTVMTACWDWKGVRALTLSDGARFGALGLLGVGSYVVAAYGLLYTNVTHYALIYSLL
                     PTFTAFFSWCLFKEQVSWSGFSGILLSWAGCLAALATNLEGGAIEVSFGDPLVFLFTL
                     MMAAHIVMSMHIVRRHGVLTANTAMFGTSAVLVSGCSMAWSEPAPAADLSWKVWSAVL
                     FIGLGTAGVFLLRCRSLQSLPPATVGAYHNLIPICTIAIAHWYLGEPLTRTTLIGALA
                     VLLGTELVRRKPPWTNVLSAVFKKESGDRAVPLVGMAVAPVAAQSTVRHGRE"
     gene            complement(18059..18382)
                     /locus_tag="VFH05_09800"
     CDS             complement(18059..18382)
                     /locus_tag="VFH05_09800"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678552.1"
                     /translation="MTNQRHQFRMPVARRGLVTKGQTTALCDVIDITEQGLHFSSELP
                     LTVAETVRIECQLDENSVVHCELLITHAEPPRFGGKMTHLLPEHRQQLVSFIQRLIMS
                     SMAGL"
     gene            complement(18379..18528)
                     /locus_tag="VFH05_09805"
     CDS             complement(18379..18528)
                     /locus_tag="VFH05_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678553.1"
                     /translation="MTPLMLITLISLAPFPILSVALLAGRWLKPHCQSVVIAHAAYNQ
                     NGPYR"
BASE COUNT         4065 a         5137 c         5427 g         4138 t
ORIGIN      
        1 ggacggggta taggtaaaag ctcgttggcg gacttgatac gtttgggtgg cgataggttc
       61 acaggacgcc cacagcagga ggcagaacgc caaataagct atcgggccga tcgtgacacg
      121 acgtcgtctc aatggccagt cggcggcttg agcccccgtc tcgtcgtatg ttggaagatg
      181 cactttgggc aggtgtaatg gatgaactgt ccgcttgcga ccagccgctt gttcatcggt
      241 accttgcacc aggtgcacag gcggtcgggt aattcagacg cgggagaagc aggagcgtta
      301 gggcttgttg acatggcggc attgtccccg agcaccaaga atcttgtcaa ccgagacagg
      361 atgaagcggt ctcgaaaaat attttcgcag tcgccgatgc agatgaaaaa aatcgtggta
      421 tagttgcaca acattccagc atgagtcttt tcacccacac acagaaaggc ggactgccgt
      481 atgagcgttc cggttggttc ggaagagacg attttgccat cgagtgcccc agcggcttct
      541 ccctccgtcc cggttcagga catggtggga gagggcaagg cctggggatt gtgcaccgcc
      601 gttgacctgc aagattgcca tcctgatttg atccgcgatg ccgagcatat acgccgctat
      661 gtggcggagc tgtgcgactt gatccgcatg cagcgattcg gcgaatgcca ggtcgtcgat
      721 ttcggctccg gacgggtggc tggatattcc atggtccagc tgatctcgac gtccttgatt
      781 agcggccatt tcgcgaatga cacgaacagc gcctatttgg atattttcag ttgcaagggc
      841 tatgacccga gcgtggtcga agaattttcc aaggaattct tccgagcacg gcgatcgacg
      901 gccacagtca cgcttcgtta ttgatcaaaa ccaagggggc ggtggaaact ccgctccctt
      961 acactctcag ccgccactcc gatcatgact gccccaacga tcaaggattt tttcaaacac
     1021 ctgcccggta agttcgatgc cgaggccgcc gacgatctcg aggcggtcta tcaatttgac
     1081 ttgagcgggc cgcaaggcgg gcaatatgtg atcaccatcc ggaagggcgc gtgtcaagtc
     1141 agggaaggca gagatgacga tccgcatgtg acgctttcca tggcgagcga agattgcatc
     1201 aaggtgttga atggtcagct cagtggaccg gccgcggcca tgtcgggacg gattaaaatc
     1261 accggtgata tgggcttggc gatgcaattg aaggcgctgt ttccgacgat tggtgaacaa
     1321 ctctagcctg cagacagccg ctctcccact caatcttcct cgcgaggacc attggcctag
     1381 cggtttgccg gcaccagcgg cgcttcggag ggaagttcct caaacacagt cggttcctgg
     1441 gtcgcacggc gcgggaagcg ttcttcgagg atgagataca tcgtgggcac gagaaacagc
     1501 gtcaagagcg tcgatacact gaggccgccg acgaccgccc gagccaacgg ggcgttcgtt
     1561 tcgccgccgg tcccccagcc gattgccatt ggcagtaagc cgaacaccgt cgcaagcgac
     1621 gtcatcaaga taggacgcag cctggtgcgg gccgccagaa ccaccgcttc ctgcagggcc
     1681 aatcccttcc gccgcagcac attggtatag tcgacgagta acacgccgtt ggacaccaca
     1741 atgccgagca tcatgatgat gcccatcatc gaggtcgttg acagcgtcgt gtcggtaaga
     1801 aacagcacca ggatcacccc gggaaatccc atcggaaccg aaaacatgat gatgaagggg
     1861 tccacgagtg acttgaactg ggcggccatg accatataca cgaggatcag agcgagaaca
     1921 gtggcgaaca tgaggcccac aaacgtttca cgctgctgct gaatctgtcc ggccaatcgg
     1981 atactgaaac cattgggcaa ttgcagtttg gcaaaggcgg tttcgaggtc ttcggcaatg
     2041 gcccccagat cacggtttaa cggattggcc gtaatgtgga tcacccgttg aaaaaacttg
     2101 cgatcgattt tcaccggtcc ggcgttcaac ttcagcgtcg cgacgttctt caggagaacc
     2161 ggttcgccgt ttctggccgt caagataatg ttctcgatgt cggtcagatc cttccggtgt
     2221 tcttctgcca gccaggcgct aatgtaatat tcattcccat tttggggatc ggtatagatg
     2281 atcggatccg tctgtccgtt cccgttgagt gaaaacagaa cggcgttggc aatgtcggtt
     2341 tcgctgattc caagcagggc ggccttctca cgatcgacca cgacgttgac ctccgggtag
     2401 ttctcctccc gactgacttc gatatcggcc aagccgtcta ccttgtgcat gaccccctcg
     2461 acttggccaa tgaccgttcg ggcgttttca aagtcatagc cgtagatctc aacgtcaacg
     2521 gctttctgtg agccgaaact ggtcacccgc ttgacgagcc cccccggatc gaaaaacatc
     2581 gccacgccgg gaaataactt gacgattttt ggccgcacgt cgttcatgat ctggacttga
     2641 tttcgcgatc gatgttccgg cgaagacagg tacacggaaa tgaccgaagt gtgcgggccg
     2701 gtgttgggat taaagagaga cgatcgaccc tgggccagaa caccggtgct ggacacgatc
     2761 gtttccaact cgtgggccgg gatagtggta cgcaagactc gctctacctc ggcaacctgt
     2821 tgctccgttt tctccacacg ctggccgacc ggcgcacgaa gcacaatgcg aaactgactc
     2881 tcatctgaaa cgggcagaaa ttcggtgcca atccgcggca ccaaggcgag cgaggctccg
     2941 aagatgacga ggatgccgac gatgaataac cggcgatgag tgaggaccca gcggagtgac
     3001 tcttcgaatc cgcggtccaa cgactcatac cgctcacgac tccacgtcat gagactgacg
     3061 aaccaggccg gcatcgaacg gtgcgattcc tgttccggct tcaggaattt gaagcagagc
     3121 gccggcgtga ccgttcgcga gacgaaaaag gaagtgaaga gagcaatcgc gatggtgagg
     3181 gtcagaggaa tcagcaacag ccgggcaatg cccacgacaa aaaagatcgg gagaaacacg
     3241 accaccgtcg tgaccgttga tgcgaaaatc ggcatcgcca cttcccgggc ggcttctaaa
     3301 atggcatccc aacgacgttg gttcgtattg agatggcgtt gaatgttttc cagttcgacg
     3361 attgagtcgt cgaccaggcg accgattccc agcgccaatc ctcccagggt aaagacgttc
     3421 aacgtttgac cggtgaagta cagaacaatg aacgtcacca tgatggagag tggaatggcg
     3481 actgaaatga tgagcgtgct ggttacgttg cgtaggaaga cgagaataac cgcggcagcc
     3541 agcagagaac catgcagggc ttgctcgacg agattgccga tcgattggcg gatgtatacc
     3601 gattgatcga aggagatgcc gagcttcacg ccgggaggaa tgccaatcat tttcggcaac
     3661 gccgcacgca aggcatcgac cacttccacg gtatttgcga tcggttgctt gttgacgcga
     3721 aggtagactg cccgtttgcc gtctgttcga acgatgttcg tttggatgtc ggaagaatcg
     3781 gtcacagtgc ccaggtccct gacccgaaca gggtttccct gttggttcac tttgacaatg
     3841 acatcttgaa tcggctcaac ggtgcggaat tggttattgg tgaagacgtt gtagtcgaga
     3901 ttgccggcct tgatatcgcc agaagggagg atcaaattcg cggctttgac cgacttcaca
     3961 acatccagga tcgacagccc acgcgcattg agcagtgccg gatccagatt aatgttgatc
     4021 tggcggatct tgccgccttc cacggtggcg gcggcgacgt tggcaatctg ttcaatctgg
     4081 ggagcgatcg tattgaacgc aaggtcatac agcgcccgtt cgtccagcgc gtcgctggaa
     4141 acagtgacga aggacaccgg aatattcgag acgtcgaact tgacgataaa cggttgaaga
     4201 atacccggcg gcaggctgtt caagatctgg gtaatccgct gcatgacctc catctgtccg
     4261 acgttgatgt ccgcgcccca gttgaaccag atttgaacgg cgccgatgcc ttgcttggcg
     4321 aatgattcca catgctccac attggatgcc gagctgacgg ctttctcgat gggatacacg
     4381 acgctctgtt cgatatcgag tggcggggcg cctttgtaga tgacaccgac aaaggcgaca
     4441 gggacctgaa tgttcgggaa cagatcgacg gggaggcgat tcagcgaggt ggcaccgagc
     4501 acgaccatcg caagcgacag catcaggatg ccgatgcgat ttcgtaacgc gagaagggtg
     4561 agccacataa tgtcgcgcgc tgagcgagac tcaatacgat tgatctatct caaggtctcc
     4621 tgatccgagc caacccggac ggcttacgag cgggtcaagg gttctgtctg tacgggcatc
     4681 ccgtcgtgaa cgaggtcttt ccctgacacg atcacttgtt cggagccgac cagtcctttg
     4741 gtaatctcga cccggttctc gtcccggacg ccgatttcaa cggatactcg ctcggccttc
     4801 ccgtcgcgta cgatatacac gtattgggcg tcttccaatc ggctgaccgc atcaatcggt
     4861 atctgcaatg cattctggtg ctttcctacc aggacctcga cccgcgcgaa cataccgcct
     4921 ttcagcaagc ggtcgttgtt cgggagatcc acctcaaccg tcatggtccg ggtggcgcgg
     4981 ttcaaggcct ggacgacgcg cgtcactgtc ccttcgaaca ctcggtccgg atacgcttcc
     5041 gcgcggatct ctgccttttg tccgatccgc accagcggaa catccttctc cacgacttcg
     5101 atgagggtac ggaccgttga gatatcatgc aaactcacaa tgcctcggga ggtggtggaa
     5161 ttgctggtgg cggcccccgt gacataggcg cccaaatcga ggttccgctc tgcgacgtat
     5221 cctgagaagg gggctcggat gtaagagtac gtcagattgg tctcggcctg ggcgagcgcc
     5281 acctccatct gcttgacctg tgcgcggagc gactcgagag cggcgatcgc tgcatccgag
     5341 ttcacctcgg cggtatccac atcttgttgt gaaacaaact ggtccctgat cagggcctgc
     5401 atgcggtcaa gcgtcagttt ggcattgcga accgatgcct cttgctgggt gaccttcgcc
     5461 cgggctgccg cgagattggc tttggcctgg ttgacgttgt gcaaataatc ctgatgatcg
     5521 atttccagaa gcaactgatt cgccttcacg aaatcaccct tgtccacatg cagcttggag
     5581 atatacccat cgacacgtga gaagacattg accacctggt tgggagtgat gtcggcggtg
     5641 taccccaacc ggatctccaa gtcttgcttc aagggggtga ccgtgcccac cgtgatgacg
     5701 cgtgatttct tttgatcgat cttggcgccg ctgctcaggc ggaacacgac aagtgccgtc
     5761 acggcaaaga agataatgat gccgagcgtg acaagggggt gctgccttac agggttcata
     5821 gtggccggct ccgccgcgcc ttcatgatgc ccgtgaggaa aaggtccacg taagtcctca
     5881 ccgttgcatc atgagtttga tgcatcggga cgccgaaaat ttcatgaagg agacgatgat
     5941 gtacgaccat gccgataaag gctcgggcgg ccagcagagg atcgacctga cgaaacgcgc
     6001 cctcgtcgat cctggtttta atataggtcg ccaggtgatc gtacagcacg cgatggtgtt
     6061 tgccgaagaa catgtcggac aactcatggc cctcgagcgc gctgaaaaga agtagtcgca
     6121 agagggtgga atccaccgag ggtttgatgc gaaagcctgc gattaaggtg aatacccgcc
     6181 ggtcatcacg ctttcgcgcc gattcctcga ccgctcccaa cagctcatcg agggtgacct
     6241 tctcggccaa gatcgctccg tagagcgcgc gcttggtggg gaagtgcttg aagacgagcg
     6301 cttcactgac gccggcggcc ttggcgatct ccttggtcgt cgtgccgttg aatcccttgg
     6361 ctgcaaagag ggaggccgcc gccgcaatga gaccggcctg acgctgctga ctggtcgatc
     6421 gtgtggccat aaccgtcatc taagtgagtg agtaattact tacctagaag gtaccacgaa
     6481 catctggcag gggacaatga aaagactgct tctccgagaa agagggagtt tatcgaggaa
     6541 gattggtgcc cagaaagtac cgcaagcggt tccgctcccg tacgttcatc ttgaggattt
     6601 ccacaccgaa ggcgtgccct cgtgaccagg tgaccttcgc actcatgacc aagaggggat
     6661 cgcgaccgtc agggagatag aggaacaagg tcacctcaag gccggacctt actgccacgt
     6721 tgccatggat gcgcagtcca ctgttggaaa ggtcgacgac actgccatca cctgtgatca
     6781 acacgttttc ttcgtcctgt gccgaataca tgagccggac atcgacggga acccgaggtt
     6841 cgttacgtct gtcacaaacg ccatgggtgc ggtgcagtgt tttcgacaat cgagctctgt
     6901 cctgcttcat ggcgtcacct ctgatcaaga tttgttggct tttttccgaa acagattgtt
     6961 ccacacataa aaccgcaact cattctgcac ttcgagtttg ggcgcaatca tctcgactcc
     7021 gaagcgccgt ccagacaccc aggaaacacg gctttccgcg atgcacacag gttcctcgac
     7081 tccggggagg tcaataaaga gcgataagtc cattccaagc ttcacgacct ggttgccgtg
     7141 gatcccgatg cctgcttctg agatgttgcg cactacgccg ttgccgatca acatgcgacc
     7201 gtcatacatc ccggagtaca tcaggctgaa ctgtgtcggc acgcgctctg tcccgcggcg
     7261 gttgtcgtcg ggtggcttgt cggcggttcg gcgtgaccgg tcagtgctcg tgtgtttcat
     7321 gccgtttcct tccatggttg aaatcgacta actcccaggc agttagggga cgatacgaaa
     7381 ggccgtccga tggtggttgg cctcgggcaa cctgacatgt ttccagatga tgccctgtat
     7441 tcgcttccgt tcgtcgtccg tcagcttggg gaaccgcacg gcgaacacat gacctttgat
     7501 ccaggatacg gttgcatccg tgagacacaa gggcgcctgt ccgtcgttta aatacagtgt
     7561 aaccgtgacg tggcctccga tcaccggaag cgtcgtaccg aggattttac agccggtttt
     7621 tgacagatct ttgagagagc cgatcgatgt ggtgaacctg gctccttccg taccggtgta
     7681 cgtgacctca tgatggatga gcgctcgttc ggcataccgc cgatcaagcg gcgcatgagc
     7741 gcggggcgcc gacaggttct gttcaagtaa gctgcatttc attggtgcct cctggttgcc
     7801 tacacctcga gaatacccca ggggccaggt tcggccattc ccaagtagag gggcaccggt
     7861 ctcctacaga agagggggca acgagtgctg aagcctgcgg atacacgaag actacgtggt
     7921 tttacctggc agtcactggc ggattttggc ccaagccttc tgatattcct cgaatgaatc
     7981 tacctccatc cagcctttga acaccggcac tgcgtcgata cggtgtccct cgtcgatcaa
     8041 ctcttgcacc agatcggtga aggaggcttt gctcacactg cgggcttcat gaaaggcggc
     8101 agacttgtac cgctcttgtg agctgcgata acactcccgc atggcctgcg ttcccttttc
     8161 ggaaaacatc gccatgccaa tgaactctcc atgaacttgg tccaaaggaa ggtgctgacc
     8221 gattttgacg atccggtgtt cgtcctcccc catgacaaat cgcgacaggt aactactgcc
     8281 ggggggatcg gccaaggtca caagatcagg gttgagatgg acgggcgctc ctccacggtc
     8341 cacatgatcc cgccaggcta gatcaataac gagggcaaaa tcggctgagc tcttcagcag
     8401 cttttccagg atcgtggtat caaaaataat gtcgccatac agcacgattg ttcggccctt
     8461 catttcgggt tcggcacaga agattgaaaa gagctctcct gtgtcttcgt aacgatcgtt
     8521 gtcgtagtag cggatatttg gcagcgcgat cgcctccttc ttgtatccac ggataacggc
     8581 gatctccttg atgttcgcct cattgagcgc cgccacggca cgctcgagga ttgtcttgcc
     8641 tttgatatcc agtaagcact tgggtttgtc ttcgattaag gggagcagct gtttttcgaa
     8701 tccggccgcg ggtatgatcg ccgtaatttt ttccccaccg atcggaagga aggccttttc
     8761 gtcctgttcc atttgaggca cgccgacaat gtcgtagact tcgggcaagg tcacaatccg
     8821 atcattggcc gcaccaggcc gcgtgtcagt ccggatgaga tcgagggtct ctcgcatggc
     8881 ccgaatagcc gctcgcacgg gttggttcgc atagatgatg atcttggcgc ccgcctgctc
     8941 catttcttga gccgttgttt gatcaaaaat cgtggggacc acaaccaacg gcacgcgccc
     9001 ggaccaggct ctgtataaag tcttcagctc gtcgaacgtc ttggacttgg agtgaatcaa
     9061 tacggcatca gcaccggcct ccgcataagc ctcggcgcgc ttcaacgctt cctcctgacc
     9121 ccaaccggca atcaaggctt cggtgcgagc gatgaccatg aaatcgggca ccgtttgtgc
     9181 cgctttggca gccttaatct tcccgcaatg ttcatcgatg gggatcaatt ctcggcggac
     9241 gccggcgtaa aaactgcacc gcttgggata gacgttatcc tcaatgcaga tcgcagcaac
     9301 acccgcccgc tcacgatcgt tgacggtgcg catgacgttc aaggcattac cgtatccggt
     9361 gtcgcaatcc gcaataacgg ggatgttcac tgcttccgca atgttacgtt ccacctcgag
     9421 ttgctcactc gatgtgatga aactggcgtc gggaatgcac ttcagcgagg ccgaaatggc
     9481 gaacccgctg gcccacacgc cgtcgaagcc ggctcgctcg attaaccggg cgctgagcgc
     9541 gtcatgagcc ccgacgacct tcagggctcc cggctttttg agggctgctc gtagcttggt
     9601 ggcgttatgc atggtgaccg ggcatgatgc gaaagcccat ggttgttgtc aatgaaaagt
     9661 tttgtacggc gggcgaggaa tcagtacgtg gcaccgccca aatcctgatc gggggccatg
     9721 gcgccttcaa tcctggcggc aagcgccgga ttttcgaaaa agatgatgcc acgatcggag
     9781 cgtgtttcgt agcggaggct gaccaccgtt tccagaccct gccaggcaaa aatcttcacg
     9841 gcaccccccg caaactgtcc cggcgtgcga tcgagtggcc cataccgtga ctgaagaaat
     9901 cccagcagtt gatcatgggc ttctctgccg cgatatcgaa ctgtgacgcg ggcgaatcgg
     9961 tcatcaaccg tggtaaatct catggattcg accttggctg gcccaaaggt cgggggttct
    10021 cctttcagtt catatgtctt caaacggccc gcatcttcca ctttaccgaa cgtgtccgac
    10081 tccgacaggg ttgcacccca gggaatgcct tcaaagcctt tggggtcatc cttcatctcg
    10141 atcgccagcg tagaggtcac gacgaggaga ttgccggaga gcaacatcgc gaggaaaacc
    10201 tgacgcagga ggaagtcccg gataaacatg agctattcag ccgtgtcggc cagcaggtcg
    10261 ttgaagcgtg gggccagcgt ccggctgtcc agaaaaatat agccgcgctc ggtgctcgac
    10321 tggtaggtca aattgatttc cgattcgctg ccgcgccaat tgtactgctg attgaggccg
    10381 cgcagcattt gtcccggcag gtgttccgtc cgaccgaatt ccgattccag gtacgagagg
    10441 atctttttgt gggtttccgc accgcggtag cggatggtca cgcgggcgaa ttggtcgtcg
    10501 atagttgaca gtcgaaggct ctcgacggga atggtcgcaa acgatggcgg cgtatccttg
    10561 aactgatact cgatgatgtt gcggccttgc cgtgtcagtt cgagatccgg ccgtgtgttc
    10621 aatgcagttc cccagacaat gttgtgaaat cccttgggat cgttggtcat tgacacggcg
    10681 cctgccggac cgggcgagaa cgcaatcatg ccgctcacaa gaaggaagag taaccgcatg
    10741 atgtaacgct atcatgctgt gtaaagtggc gcaacggctt cgtttccttg tgaaatcctc
    10801 acggcatggc ttataatgcg ccttttttcg gcaggatatg attctcgaag aaaggtcaga
    10861 atgatcgaga gcgatgcatt cgtacaggcc ttgcaggata tgggtgtcaa tttcttcacg
    10921 ggggtgcccg attccatgct cggaggcatt atcgccgaac tgatggaccg gcgcctgtat
    10981 acgcccgccg tccgtgagga cgaagccgta gccatggctg ctggcgccta catgggcggg
    11041 aagattcccg ccgtgctgat gcaaaattca ggactgggga cctcactcaa tacgctgatt
    11101 tccctgaacc tcatctatca tcagccttgt atcctgattg tgtcttggcg cgggcagggc
    11161 gggaaggatg ctcccgagca tctcgtcatg ggcgaagtca tgccccaact gttggatacg
    11221 atgaaaattc cgcaccggac gctgaccgaa gccggcgcga tcgaagactt caagtggata
    11281 gcggagacct tcatgaaaca gcggatcccg gtcgccttgt tcatcaccaa gggagttgtc
    11341 aaaggtttac atccatgagg ccggagcaag ggacattgat cagccgtgcg caagcgatgg
    11401 cggcgctgct tgaattattg accgatcaac cggtgatcat ctgcaatggg ttcccttccc
    11461 gcgaggctca gaaacttgtc gaccggccga cgcattttta catgatcggt tccatgggaa
    11521 gcgctccggc tatcgccttg ggcgtcgccc tggccaagcc gcacaagcag gtcattacgt
    11581 tcgatgggga cgggaatgtc ttgatgggta tgggcacgtt ggcgacggtc gccgccctga
    11641 agccgaaaaa tttcatccac gtggtctttg acaatgaagt gtacggtacg acaggtaatc
    11701 aaccgacgat ctcaaatgtc gtgcctttgg aaaaggtcgc caaggcggca ggctacacga
    11761 atgtcgagcg tgtactggat ctcgatgatc tggtctatga gttcaaagat atgctcaaga
    11821 aggacggacc gagtatgcta ttgatcaagg tcaatgagtt cgcagaagat gcgggacgag
    11881 ttgtgctgga ccctcccgat atcacaaaac gatttatgaa ggcgatagaa taagaccgct
    11941 aaccccatgg acttgcctat gactggactt ttcacagccc ccattgagtg ctcgtcgccc
    12001 ggtttcgcca cacctcgatc gtcggggctc ggttctgagg ccgccttatg atgctgttga
    12061 accccgggcc agtgaacgtc tctgaacgtg tccgtcaggc gctgacccgt ccggatatct
    12121 gtcaccggga atctgatttc gccgaactac agcaaggcat tcaagcgaag ttactgaagg
    12181 ccttcgtgcc gggtgcggag gccgagtatg ccgccatcct gatcaccggt tccggcacgg
    12241 cggcagtcga atcggccttg atgtcctccc ttccgcacgg gaagcgcgca ttgatcatca
    12301 acaacggcgt ctacggcgaa cggatgtcgc agatggtggg cctgcaccgc ctcggagtat
    12361 cagaactcaa acatgaatgg accgagcgac cggatcccga gcggttgcgt ctcgcactca
    12421 ggcagcatcc tgaagtgcat gcggttggaa tggtccatca tgagaccacg accggtttac
    12481 tcaatcccgg caaagcgatc gcagaagtcg tcgatggtct caaccgcgta ttcattctgg
    12541 atgccgtcag tgccttggct ggggaaagta tcgacattgc gggttcaaaa atctatatgg
    12601 tggccggtac ggcaggcaaa tgcattcaag gatttccagg cgtgtcattt gtccttgtgc
    12661 gcaaaggttt tttggagcgg atgcgagcgt atcctaaacg gtctttgtat ttgcacctca
    12721 cgcactatgt cgacgatcag ggccgcgcga cagtgccctt cacgccggct gttcagctct
    12781 attacgcttt cgacgaggcg ctcagcgaac ttttggaaga aggcgtcgcg aagcgcatcc
    12841 agcgctacaa gaagatggcc cagttgatcc gagaacggat ggcgaagatc ggcatcaaac
    12901 cggtgctgca gccggaccgg caatcgaaca gcatcaccgc ctactatctg cccgaagggg
    12961 tggcatatcc cacattgcac gatcggctca gggccatggg ctatgtcatt tacgcgggcc
    13021 aaggcaacct cgagaacaaa atttttcgca ttgccaatat gggcgccgtg accgaagcac
    13081 aattcatcgc gtttctcgat gcgttcgaac agattgtcgg cgggcatcca taacaaccgg
    13141 aagcacaggt ccgcgaggag ctctgcaagc tcgtcccctg cgcaggtaag cggacctgct
    13201 gaatcgggac ccagcttgtt caacgacgat gccaagctct agggttcgat ttccacttca
    13261 tgcttaaggc aagccgctgc atatgaaagc cgtcattctc gccgccggag tgggaaagcg
    13321 cctgtggccg gtgacacagc atcgtcctaa atgtttgatc gagcttggcg gccagaccct
    13381 gctgtcccgc tatctatcct ctctcgcgag cgagggaatt cggcgtgccg acattgttgt
    13441 gggctataag caggagatga tccgcgaggc cgcgaattcc gagtccttcg gcgtgcaggt
    13501 aaacttcctc gtgaacgagc aattccaccg aggcagcatt tcctcacttt gggtggcacg
    13561 tacggcactg gatgacgacg tcgtgattat ggatgccgat gtgctgttcc atcgggaaat
    13621 tctccgccgg ttggtacact caccgtaccc gaacgcgttg ctgatggatg attcggtcaa
    13681 gcaaacgggt gaggagtgca tggttgtcgc tgaaggaggc cgcgtgatcg ccctcacgaa
    13741 aaagttgccg ctgaggtatg atcatgcggg cgagggtgtg ggttttctca aagtgcgcca
    13801 tatggacacg cctcatgtgg tggcctcgtt gagaaccttc gtggatcgtg aggcatggca
    13861 gatggaatac gaagatgccc tcatcgaatt ctttcgcgaa gtaaaggtcg gctacgaagt
    13921 cattggaggg cttccctgga ccgaaatcga cttccctgta gacgtggaaa aggcagaacg
    13981 ggacgtctta cccaagttgt agcgacgtct gttgcgaaat tcagttcgac gcctcggaat
    14041 cttttccctc caacgagtat atccatgact gagagagcat tggacaaaga aacggaactt
    14101 cagggactct cgaccgccgt cttgatgccg tcgattgatt tgcttggagc cccggagcga
    14161 aaggctgtgg acccgctcac gcccatcgtc ggcgtcggac tgttccaacg cactgtgctc
    14221 acgcttcagc gcgcgggaat caggcagttg atcgtggttg ccggtcagga agaagaccgg
    14281 ttgaaacaag ccctgctcaa gggcccccgc gtaagcattc cggttcgttg gatgccgatt
    14341 cgcgaatttc cgttggacga cccgcgcacc tgggaatcgc ttgcggcgga agtgcgcggc
    14401 ttttgcctct tatcaggtgt cgccacggtc ttttcgcgtg cgctgattga acaattacgc
    14461 cgtgatgttc aggaaggact ggctatcatc gtgacacagc gcgagcatcg accggatgtt
    14521 caaaagagat ggctacacga gggatggtcc ggtatgagtg acgggcttgc agacgttctt
    14581 gttgtgccca caacggaatt gagcgcagcg gcacaagtgg cgggcgaatt ggaaaccgtc
    14641 cctatcagtc gctggttgca gcgtgccatg gtcgatggcc gcgtacgggt gacctcaatc
    14701 gacgccgatc cttcatgctg gtatcaaacg gttgtcacgt ccgaagatct tcagcgggca
    14761 gagcatcgat tgtacacttc cttgaaaagt gagttcgaag gattcgtaga ccggtacttt
    14821 aatcgcaaat tgtcacgttg gtgtacgcgc gtgttcatcg cgatgggatg gtcccctaac
    14881 gcgatcacgc taacggccac cgtgatcgga cttgtgtcgg ccgccgcgtt tgccgtgggc
    14941 acctacgccg ccggtgtcgc cgccgcgctc ttgttccaac tggcggcagt catcgactgc
    15001 agcgacggcg aagtcgcccg cctgacattt accgattctc cgttcggagc atggttggac
    15061 atcacgttag acaacctcgt gcacatggcg atcttcggcg gcatcgcgat cggggtctac
    15121 tcgaatgcgt ccggcagcga atatgcctgg gttgggctgg cgctgggcgc cgcggccatc
    15181 ctcggcaacg ccctctcctt tgcgttggtg aacaaggcgc aaaagatcac aacgggacgc
    15241 aaatgggatt ccccgaaaca tgcggccttg tccgaattca tgctcaagaa tgtggcgagc
    15301 cgggattttt ccgtactcgt gctgctcttt gcggtgtttg gaaaactgga atggtttttg
    15361 tggattgcgg cgatcggatc gatggtcttt gcggcagtca tgctgtggat cgtgagaccc
    15421 tctggcctct tcctgagacc tgtacggtga tgctggagtg ccgctctgct cagccaattg
    15481 cacgtgtgca catcacgcga gggaagccgc tgtgagtgag gaccgtagtc tcagggtttc
    15541 caagtctctg gtcatgggag agcaaagccc ttggccattt tcacccgcct aaccgtgttc
    15601 cgatatctcc tactggccgc cggtcttctc acgctgtcct ttctcgtgtg gcatataggt
    15661 ccggtgcaca tttatgaggc ggtcgcccgc ttcgggccgg gtgccctcgt cgtaatgctc
    15721 attccatctg tggtcatgta tgtgctcgag gcctatggct ggaaaattgt cttgggttca
    15781 acggcagagc gcatttcctt tggcaaaatc ctctcaatcc ggaccgccgg tgaagtggtg
    15841 aatatgacga cgcctactgc ctacatcggc ggggagccgc ttaaggcgta cctccttcaa
    15901 aggcaccaca tcccgatggt cgagggcctc gcctcggtcg tgattgccaa aacgattatg
    15961 acgatcgccc aagtcttatt catcttgatc ggaatcgcgc tggccttctg gacgctgggg
    16021 gcggccggat cgtcgctcca gatgactgcc gcggcactgc tcagcgcggc cttgttgtca
    16081 tgtgggattg ccgcattcgt cctcgtgcaa agacgcggac tgtttacgtg gttgctggag
    16141 acatttcgcc gggttggcct ccgcatgacc tttcttgaag tgcgcgccga gaaacttcgg
    16201 tcgctcgaca ggacgattct ggggttctat gcccatcatc aatcggcatt ttatgcttca
    16261 acgggtctgt atctgctggg atggctcgct gaagcagtgg aagtcttcgt gatcatttcc
    16321 tttctcggcg ggcctgcgag tcttttgtct gccactgcga tcggggcgtt atccgtcttg
    16381 atcaaaggcg ggacattctt cattccgggg agtttgggtg ctcaggatgg aggtaatttg
    16441 ctcttgctga gggccttcgg gtactcagac ctgacgggaa tgaccttcgc gctcttgagg
    16501 cgtttcaggg aattagtctg gatcataatc ggtctggctt gcttggcgat tctcggaaag
    16561 aaatcacctg gcagtcagcg accgaagtcc taggacgatc ctgagcgctg ctgctgcagg
    16621 aagtgaatcc gctcgatcat cttgtcccag acaaacgaat gcaagcgcaa gtttgcttcg
    16681 cggtccacgt ggacgaattc gatgccggca tgtgtgcctt tgacccattt gacttcggct
    16741 gcgtcgatgg gcagggattc gctttgatcc ggaagaagga gtcggagtgc cacttgttga
    16801 ttctcctgca gcggttcatc cgttttaagt gagcatccca cagccgacag gttggatatc
    16861 gtaccttctc caacggtggc tgccacacaa tacatgaccg ggtagctcag cggcaggcga
    16921 tagaggccgc ggtggtaggg cctgctctct ttcatcgtag cgtcagtcta ctgtgtcgcc
    16981 cgtgaccgaa tgtatccctc cttagagggg cgacatcatt cacgaccatg ccggacagtg
    17041 ctttgagcgg ccacaggagc aaccgccatt ccgaccagcg ggacggccct gtcgccgctt
    17101 tctttcttga agaccgcaga caacacgttg gtccacggcg gcttgcggcg gacgagctca
    17161 gtgcccagga gtacggccag cgctccaatc agggttgtcc tcgtgagtgg ctcacccaga
    17221 taccaatgag cgatcgcgat ggtacagatc ggaatgagat tgtgataggc gcctacagtt
    17281 gcaggcggca aggactgcag ggatcggcaa cgcaagagaa agacaccggc cgtccctagc
    17341 ccgatgaaaa gcacggcact ccagaccttc catgacaaat cggccgccgg cgctggctca
    17401 ctccaggcca tcgaacagcc cgataccaaa acggcactgg ttccgaacat ggccgtgttc
    17461 gctgtcagca ccccatgccg tctgacaatg tgcatactca tgacaatatg ggctgccatc
    17521 atgagagtga agagaaacac gagcgggtct ccgaaactaa cctcgattgc ccctccttca
    17581 agattggtgg ccagcgctgc caggcaccct gcccatgaga gaaggatgcc ggagaatccg
    17641 gaccatgaca cttgctcctt gaagaggcac caactgaaga acgccgtgaa cgtgggcagt
    17701 aggctgtaaa tcagcgcata gtgtgtgacg ttggtgtata agaggccata ggcagctacg
    17761 acgtaagatc cgacgccgag caacccaagc gcgccgaacc gcgccccgtc agagagagtc
    17821 aaggcacgca cacccttcca atcccaacaa gctgtcatca cagtcagaaa aagaaagcca
    17881 atgacgattc tcccgttggc gattgacaaa aaggtcaggc cgctgtgctc aagcacgtac
    17941 ttcatcgccg gcgtgatcgt gctgagcaat gccacaacgc cgagcatgct caagatgggc
    18001 acggcttgcg agggtgatcc ttcactgtct gaaccacccg agtgccaagg aagcattgct
    18061 atagccctgc catgctggac atgatcaagc gttggataaa ggagaccaat tgctgccgat
    18121 gctccgggag cagatgcgtc attttcccgc cgaagcgagg aggctcggca tgtgtgatca
    18181 gcagttcgca gtgaacaaca gaattttcgt ccagctggca ttcaatgcgg actgtctccg
    18241 ccactgtcag aggcaattcc gaggaaaaat gcagcccttg ttcggtgatg tctatgacgt
    18301 cacatagagc cgtcgtttgg cctttcgtca ccaggccacg tcgggctacc ggcatgcgaa
    18361 attggtgacg ttgattcgtc atcggtacgg tccattctga ttataggccg catgtgcgat
    18421 caccacagac tgacagtgtg gcttcaacca gcgcccagcc aggagcgcaa cactgaggat
    18481 gggaaatggg gcgaggctga tcaacgtgat gagcatcaaa ggtgtcatgg tcttctcctt
    18541 ggcagagagg tcgttcgccc ggacatggag cattgacagg gaatgtgcaa tgcaggggcc
    18601 agaggaatac ctacaaagaa gggcataaga ttccgctgca gatacaagcc cttggcggaa
    18661 ctgtggcacg gtcagtcagt cgtgacggca gaggaaaaag agacgtcggg tgaagaagca
    18721 gacggaaatt taccccgctt ttgccaagaa tttcctccct cttcaga
//
LOCUS       DASSCQ010000145        32286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_659833, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000145 DASSCQ010000000
VERSION     DASSCQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 32286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32286
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659833"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFH05_09810"
     CDS             complement(<1..227)
                     /locus_tag="VFH05_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678554.1"
                     /translation="MGLVLAVSVVLWFCGLEQPASAQTISSVRSFDGGVAPLFSLTGP
                     GSLYLDNQGTQGFLYNPGNNFQSYSFRNPTTG"
     gene            311..1876
                     /locus_tag="VFH05_09815"
     CDS             311..1876
                     /locus_tag="VFH05_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6678555.1"
                     /translation="MRSRLAAGLPGVLLLSAASTLAMTAGPALGADDVTKATPGERRE
                     EISLSEAALRALKNNLDISISRHTKESRLTDIVVEQSKFDPTLSVNGQYNRTVNPLNR
                     PVFGGTGGSLNEITIFDQRNGSVTVDATTNLLTGGNVDLNYSPARTNVNQDVASGFLF
                     NPAWTGGLALTLTQPLLRNAGIDINKTFIRIAQNNAMVEEHVFRDRVLTVVATVEQTY
                     WEVVFANENLKVAEAALKAAQELLATNRAKAKAGVMSIVDVLQAEAAVASRVEQVLVA
                     EKSIRDQEDLLRRLLNPGEEELRQDVRLTPKDHPVVALEPISLPEAIDLAIDLRPEIV
                     QAKKNLETSELNTKFAKNQLLPTLSFQGTMGLAGLGKDYTDSVNRNFSGDFYNYGAGL
                     VLSYPLGNRSAWSTFNRRQIEGKNAETSLVSIRQQIIIGVKEAVRRVQTDFKRIETTK
                     SARIMAEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAIVDYNKSLSNLSRH
                     KATTLERYSLQVQ"
     gene            1883..2593
                     /locus_tag="VFH05_09820"
     CDS             1883..2593
                     /locus_tag="VFH05_09820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6678556.1"
                     /translation="MTPPGRLGISVQANERGALLALLPIAATIDYYALPDSLQEQVLI
                     QFAPQLIGYLALVLWAVHNDSVLRRLGLERGLWNRGAKLGIVTGLLLGCLNSLVILQV
                     LPWLGWDITFLTQTPHARVPVMVMVPWCIAVIAIVVEINFRGFLLGRLAALESGLWPQ
                     PALKQLSPAALLTSALVFATDPFMVNTFKQLHWIALWDGLIWAVLWIRTRNLFATIVA
                     HAIEVIIVYSAVRTVLMS"
     gene            2596..3615
                     /locus_tag="VFH05_09825"
     CDS             2596..3615
                     /locus_tag="VFH05_09825"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678557.1"
                     /translation="MTPSFSSYLVNDCFGDPGVYVEVRWSKRALLFDLGQNDALGPTR
                     LLRANDIFISHTHMDHFIGFDAVVRVALGRGKKLRLYGPPGLIDNVHGKLRGYTWNLV
                     DGYPLIIEVREFDHAEVRQATFHAANGFMLSTPTIHPLVVHPDTGLFTVLEDPMFTVK
                     AVALNHRIPSFAYALEEQFHINVNKQKLHEAGLPVGGWLRDVKQYVWAGKPDDFRFTA
                     LLFDEHRQMEQEFVLGDVRNRFLTISRGQKLAYVVDARFDEANEARIVALAAHADLFY
                     CESPYLDADAEKARDRYHLTARQAGIMARKAQVRDLIVFHFSPRYTGQGEAIEQEAHD
                     AFLGH"
     gene            3626..3964
                     /locus_tag="VFH05_09830"
     CDS             3626..3964
                     /locus_tag="VFH05_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HET6678558.1"
                     /translation="MNELGKYAVYFLLGGTIVSVSTYLGAQGRPYLAAFASTFPAITG
                     ATFILLYVNGGSDAIVAYAEKLFWFVPPWIIYVLAMIFIVPRLGFWPAMTLSLVLYMG
                     CVWLVRLAVR"
     gene            4143..4661
                     /locus_tag="VFH05_09835"
     CDS             4143..4661
                     /locus_tag="VFH05_09835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678559.1"
                     /translation="MISLVEFVAICSVATLTAAGASQGWDRLRRPRVNRSTDREKSRD
                     DGSGRPSDGAWHEPRYGKRHAVRCAIEYQVGAQKQEGILIDVSRRGWRATGPSPVLAG
                     TSLSVRVFLPDTAQSVFIEEAVVRWSDGLDFGVELMRISPEDGQVLSEYLSVHYPMEP
                     SPSHAFSPFSYN"
     gene            complement(4755..5333)
                     /locus_tag="VFH05_09840"
     CDS             complement(4755..5333)
                     /locus_tag="VFH05_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678560.1"
                     /translation="MTQPIVPTDYLGIGRQLARLLGSSLEGPSGEALKELARAYDPSA
                     NEARISAEVFLFHKYLIVQTCVGMFPSPEVERVIGGLFAALNERATGLEFSQERQNAM
                     ERMWQMRAKQFDQPFTQDREHFLDEGSDGVFWKRSLTQFCQNVLEIDHPPDIWAGGKG
                     PSHKASTCVTEAVDQLVAAMTEMSKLHFGSAA"
     gene            5516..8365
                     /gene="uvrA"
                     /locus_tag="VFH05_09845"
     CDS             5516..8365
                     /gene="uvrA"
                     /locus_tag="VFH05_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET6678561.1"
                     /translation="MLRREFDVSHLPDAESASQALIVEGARQNNLKNISVLIPHNQVT
                     AITGLSGSGKSSLAFDTLFAEGQWRYVESLSSYARMFLDKVARPDVDRVLNIRPAIAI
                     EQKNQVRTARSTVGTTTEIADLLRLLFAKIGKPICPDCHEEARSFQPDTVTSDLLERF
                     AEAKAMLLFPVTPPSAKSESAFVQSLLTRGFTRLRMGHETIDLHQDALPPSVHGQSSL
                     SVVLDRVVIREDNRGRLCEAIEMAFREGDGRCDVEVIGHGLQQYHAGFCCQRCGRTFE
                     PLRPILFSFNHPLGACPDCKGFGNVLRYDPALVIPDHSKSLVQGAIEPWSKPGTDWWQ
                     KQMLLAMKRKGVDITRPFKLLDEDVKTLLWKGDKTFDGIDDFFQYLEGKRYKLHVRVL
                     LSRYRTPFDCPGCHGSRLKPTARFVKIGGVDIHDVTAMTIEHLMAWLQGLALRPYERG
                     IATDLLRQLTAKLGFLIRVGLGYLTLGRQTKTLSGGEAQRVSLANQLGAHLVGTLYVL
                     DEPTIGLHARDTDLLAGILKDLAAAGNTVVVVEHDRQMIESADHIIELGPHSGEKGGE
                     VVCAAPAGEFLRDRRAMTARYLRGEEHIPLPASRRSGTGKVLVIAGASEHNLKDLVVR
                     FPLGMLICITGVSGSGKSTLVEDTLYAALARAFRTKTLPMGRFKAIRGIEHLKGVRLI
                     DQQPMGRTPRSNPVTYLKAFDEIRRLFAGEREALRQGFTAGHFSFNAGGGRCERCEGA
                     GVEKLEMYFFEDIYAPCERCEGRRFKPEILAIRYRGKTISEVLALTVDEARSFFSGTP
                     KLQERLHLLSSIGLGYLRLGQPATTLSGGEAQRLKIAAELKDAGAKDLLYIMDEPTTG
                     LHMDDVKKLLVVLQKLVNAGNTLVVVEHNLDVIKSADWVIDLGPEGGQAGGHIVAEGR
                     PELIAKCDASYTGRCLAQTMTEAVDRFILEPHPIA"
     gene            complement(8337..9713)
                     /locus_tag="VFH05_09850"
     CDS             complement(8337..9713)
                     /locus_tag="VFH05_09850"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HET6678562.1"
                     /translation="MERARGQSGADALIKKLHERPHIPLADWLRAPAHVHYKAFRMAD
                     PPADRKASREEFQSCLTAFGVPKDETVLHETFGYGVKQAGNGERLIIIWQAHTEYYNY
                     QIWHLPDGVHREIAFGPLTFPEYTFPISPLGTEVCRLDILLSSDSLPPREQMRSMMPG
                     PVLYGSRILDEQTSLVTSFTHDEFERERYWVALGPTRAQASRLKEIVDAIVRIETYYH
                     LLLMQKPLFSAAIDQVYKFEQVHLKQREIISGHIAHADPQTLQRWLNSLTQDLLKTNR
                     IAGKLHFELSASVPYDKIVHTTLASLNEKALDAYRPVSDYVLSGVTGVAEGYQQLLKR
                     IDTLRAGFEGIISIIRARVDLILEAQNLALLQSVDKTTKSQVLLQHTVEGLSVIVIAY
                     YVAGLGGYIFKGLQEIGWLRNANLTSAIFVPIAIGLAFGITTFSKKLLHRKLSREQPP
                     TQSGAAQG"
     gene            complement(9740..10927)
                     /locus_tag="VFH05_09855"
     CDS             complement(9740..10927)
                     /locus_tag="VFH05_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HET6678563.1"
                     /translation="MPTLFFGCGLAYTIVKSESKLDNLSVPMTKRQVIDEIGRPDRVL
                     RDDGRMLVWEYSLTARRQWLYELGLCPVSVWIGGCIIYPFTNVAMEHEREYPHHVVLV
                     NEELCAWGTPSAILQRRRACESTGMHPGRPPGAAGRPEPVVTGLGPINRDTVDRYQTM
                     AVMLLEDAPGVAGSGSRVAGIVTTLLLDLDINVVERAKLDEVLKEQVIQLTHADDANV
                     LKVGKLVGAQAIIVGGVQEWERHEQSRMNKVSLSFRMIDVETGQLLFNGEGHLTDFTT
                     DDPEGSARLIVHRILARFGSQTGLLGSGRIGVNWELLEDQGARFYLVRELRSGLPAER
                     AGLKVGDHIVACNGASLSGARNDREAKHLCQVQAGGTLQLDVSRANHPVRLSVTAEKR
                     PGL"
     gene            complement(11176..11748)
                     /locus_tag="VFH05_09860"
     CDS             complement(11176..11748)
                     /locus_tag="VFH05_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET6678564.1"
                     /translation="MQIPLRAQLGKGIFLVAAPNLRDPNFRQTVVLLCEHGAEGALGV
                     VVNRPTAMSVSEALPQMPVLEGQRHVLFSGGPVQPNQVMLLYRLDQLPDNSHHVFDGV
                     CLGGDMELVDKILTTTHGKEAFRAYVGYSGWGPGQLESEMKIGSWITVPADPATVFEK
                     DPTRVWSEILTTLGEEFRVYADMPFDPSLN"
     gene            complement(11811..12599)
                     /locus_tag="VFH05_09865"
     CDS             complement(11811..12599)
                     /locus_tag="VFH05_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET6678565.1"
                     /translation="MRVGYYQNNPEFGKVAENVDRIAAKLEDAEADLLVLPELCASGY
                     QFVSMDEVRALAEPIPAGQTTKRLLDLAKRRQMVIVAGVPEQAGAAYYNSAVVVAPQG
                     FIGSYRKTHLFFEETLYFTPGDTGFHVWDIGPAKIGVMICFDWYYPEAARALALKGAD
                     IICHPSNLVLPNCPDSMPVRCLENRVFAVTCNRTGSEARGGKDRLTYIGNSEVVSPRG
                     AILQRAPRDQEALCVVDIDPAEARNKSLTPYNDLLKDRRETFYR"
     gene            complement(12770..13105)
                     /gene="smbP"
                     /locus_tag="VFH05_09870"
     CDS             complement(12770..13105)
                     /gene="smbP"
                     /locus_tag="VFH05_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HET6678566.1"
                     /translation="MTMSIRRGAAAMAAVALLGLPLVAQADNKHVSEALEHAKSAVNH
                     GKQGHADAVAQHSEEALKHAQAAGVKNPHLDEGIKHLKEAVTHGKAGHADQATEHAEG
                     AVTHLSEVK"
     gene            complement(13195..14073)
                     /locus_tag="VFH05_09875"
     CDS             complement(13195..14073)
                     /locus_tag="VFH05_09875"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET6678567.1"
                     /translation="MNHPLLIDTETLQKQLGKPKLVVIDVRGNAAYEYGGHIPGAVHT
                     TWHQYSDPNAVAKGLLDPDMNRMEQKMRALGLSEDSEVVIYSNPFDNWGDEGRMFWML
                     EYFGHRNMRILDGGWVKWITEQRPFEHGRVTPKPGNFSVKPATHTIIGKDELRAMVKH
                     PPPDMKILDARSLEEYLGKEVSGIPRPGHIPSAIHLSWNGFLNTNATVKDLTAIKAAL
                     EEKGLDPDHDIVCYCTGGVRSSWLYFVLKLAGFQKVRNYPGSWWEWSRDFACPVEKDF
                     QGLQKVLGIEAPARPS"
     gene            complement(14083..15375)
                     /locus_tag="VFH05_09880"
     CDS             complement(14083..15375)
                     /locus_tag="VFH05_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET6678568.1"
                     /translation="MSRWKFIVLGLLVVFGATYLYYTEIKPVVIFGLRSDYAHAIPFQ
                     RVPEGLTSLKAESCGQCHREIYDEWKSSIHARAYEDPYFQAYWKKDKNVWVCLNCHAP
                     LENQQPTLITDIPRGRVEKAVQEPNPHYDAEYQKESVTCAACHVREGIIYGPFEDSAA
                     PHPTQFDPNFRTAQLCYRCHNVVSGPAQFYRVGPCGTYAEYEGKFFMQERGFICQSCH
                     MPEIDRPVATNSPIRRGRQHLWRGGHDPDMIKRAVAVEVKADPPSPKPGDRVAFTLTL
                     VNAGAGHKIPTGDPDRFFTVEFSVEDRLGHVLKEETSTMGRWIMWQPAILELYDNRLL
                     PLASREYPFAYQVPDKAEGLKLKTRVQYHIVTDKQHEMLQRTYGLTGNDPYRFVIYER
                     EFPLTDQLKAALDANVRLPVAGSSRHGSSCAVDTVGRG"
     gene            complement(15372..16328)
                     /locus_tag="VFH05_09885"
     CDS             complement(15372..16328)
                     /locus_tag="VFH05_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET6678569.1"
                     /translation="MDDEIMRTGLVYHPAYLEHDMGAGHPESPNRLRAIMQQLEQSGT
                     AARVTRIEPRKAEDEWITQIHSPGYLAALNRHAPTSGRVALDPDTSMSPGSLPAAYLA
                     AGGALAGVDAIMQGQVEHVFCAVRPPGHHAEADRAMGFCLFNNVAIAARYLQNKYGLR
                     RVLVVDWDVHHGNGTQHSFEQDPSILFFSTHQYPHYPGTGRASEHGTGAGEGFTINVP
                     MEAGEGDDEYRATFLQALLPAAEEFKPDFVMISAGFDAHRDDPLAGMGLTEAGYADLT
                     GLVAGVAKRHAQGRILSCLEGGYNLTALAASVDAHLKALLAA"
     gene            16516..16836
                     /locus_tag="VFH05_09890"
     CDS             16516..16836
                     /locus_tag="VFH05_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678570.1"
                     /translation="MPNPRIEPLKRVLGMDPNDEVAWFGLGKAYMDDGNFEEAAKALR
                     QCIIVKPTYSAAYYALAQSLHKLNLVDECRTVCGTGIEVSTKNGDAMVTKNLEQLRNS
                     LQSS"
     gene            complement(16824..17666)
                     /locus_tag="VFH05_09895"
     CDS             complement(16824..17666)
                     /locus_tag="VFH05_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678571.1"
                     /translation="MARFRPRKTFRLVSHLREGHREPLPGWKREEQGLAHDLEERWNT
                     LHRHCADQSSHLRLARLVRRITNGDYGVRESALAELELATQLIRIGAKVTFLPESQAR
                     TADLECRFGRERFFVEVTAMVGSSGRQRIPRRLLPDDEWSEGMDPGAMLIHRVLARIQ
                     QKTKQLTDYADAVVLSISIPRADLRGERTVRSEVLWLDLKALAGSVTLLLTKLRMLSA
                     VMISLWDVEPMPAQAGTRLANVELVERSKQQRGFPRVRILIQNPAATAPLTERQQEGF
                     CQLL"
     gene            complement(17669..17998)
                     /locus_tag="VFH05_09900"
     CDS             complement(17669..17998)
                     /locus_tag="VFH05_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678572.1"
                     /translation="MNDHEINRAVQYVTASTCYGRETVATILKTGFGELTDLAVQSKR
                     LFERDALLEYVSQWTIRRTGQPEPLVREVLSCAGRWLDEVYEELSPTPSEPPGPAPHD
                     DEGGEPV"
     gene            18139..19932
                     /locus_tag="VFH05_09905"
     CDS             18139..19932
                     /locus_tag="VFH05_09905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET6678573.1"
                     /translation="MAAMKKPRQTPLSNPPDRWNLSDLVNEPVRKLEQHLASLEADVA
                     QFEAARETLRPDMASSEFQSIVKVSERVTGNVSRLGAYAYLWFSENTKDAKARSFKAQ
                     VEEHLTVLQNRLLFFDLWWQGVDEQNAARLMADAGDVRYHLETIRRYKPHTLSEPEEK
                     VINVKNVTGRSAVNTLYDLVTNGLTFTLRVNGKKKLLNREELMTFVRSHKASMRQAAY
                     QELYRVFFAQRDLLAEMYKTLVNDWKSENLSLRRFTSPIATRNLGNDVPDGAVEALLS
                     VCAENADLFQQYFKLKASICGIRNMSRYHLYAPHRSETKRYRYGDAVRMVLEAYRKFS
                     PKLADLAQQVFTDRHIDAPTRSGKLGGAYCYSVVPGLTPYVMLNYTGDARDIATMAHE
                     LGHAIHGMLAAHHSVFTFHSTLPLAETASVFGERILSDALMQQETNKKVRQGLLLSQL
                     DDIYATVLRQAYFVRFEKTAHEMIAGGATGDQLAQAYLGELRQQFGKAVKVPNEFQWE
                     WLTIPHIFASPFYCYAYSFGNLLVLALYRMYQQEGPAFVPKYLDLLATGGSRSPERIL
                     SAVNVDMTSKQFWQSGFTTIREMVGQLEATL"
     gene            20072..20278
                     /locus_tag="VFH05_09910"
     CDS             20072..20278
                     /locus_tag="VFH05_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678574.1"
                     /translation="MYLCLCKGIKDSDVRDAGRAGIVMPCQIKARFGLKDSGCCGRCS
                     KNIQAFVELATSETCVPCKNTVSS"
     gene            complement(20402..21028)
                     /locus_tag="VFH05_09915"
     CDS             complement(20402..21028)
                     /locus_tag="VFH05_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6678575.1"
                     /translation="MNRRDAIRSISCLILCLLSLLSPFGCGFVRAEEPAPSAVKVLVA
                     YHSLSGNTERMAEAVVQGAKSVSGTDVVMKRVTHVTADDLFSSDALVVGSPVYWSNMS
                     GEVKTFFDNWQFKFGVFPEFRMKNKIGAAFATGGQISSGKEVTMLTILAAMLGNQMII
                     VSAGGAFGASATTEGESHGIDKKELADAQALGRRVAEVAGMLKRRPSE"
     gene            complement(21025..21210)
                     /locus_tag="VFH05_09920"
     CDS             complement(21025..21210)
                     /locus_tag="VFH05_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678576.1"
                     /translation="MKEVRKVHYVKGVFVCPKCRQSYVQEKWIESWRVRCHRCDYRGA
                     LTDVSVEERMEEETFRR"
     gene            21319..21780
                     /locus_tag="VFH05_09925"
     CDS             21319..21780
                     /locus_tag="VFH05_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET6678577.1"
                     /translation="MPPVHTDWVIAAVLALILALPSCTQADDGLIPIQLPGGVIIQAE
                     IASTMKKRAEGLMYREHLAKDRGMLFTFDQAQPWTFWMKNTKIPLDIIWMNEKKQIIH
                     IARNVPICTRTDDGCPQYQPNDPATYVLELGGGEAERLKLEKGTTLQFRIP"
     gene            complement(22024..24201)
                     /locus_tag="VFH05_09930"
     CDS             complement(22024..24201)
                     /locus_tag="VFH05_09930"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET6678578.1"
                     /translation="MVNQTVRDIDQLLDRVKSYNAEADLNIVRKAYEFSAKVHEGQRR
                     RSGEPYLQHPIAVAGVLTFLRTDVTAIVAALLHDTLEDTVATPEELESEFGKDVVHLV
                     DGVTKIGKITFKSHEEKQAENFRKMLLSMADDIRVVLIKLADRLHNMRTLEHLGQAKR
                     HAIAEETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYEVLRTRVAKRDEDRQQYI
                     QEVIELVTKALQEHGLPGQVYGRPKHLYGIYQKMNKQSISFEEVYDLTALRIVTDTKM
                     NCYALVGVIHSLWRPVPGRFKDYIAIPKSNLYQSIHTTVVGPKGEHVEFQIRTEEMHR
                     VAECGIAAHWKYKEQGRVTDRDSKTFGWLHQFVEWHRDLPDNRQFMDSVKLDLFHDVV
                     YVFTPKGIVKELPRGSTPVDFAYAIHTEVGDHCVGAKVNGKIVPLKHQVESGDTIEIL
                     TSPTQTPHKDWLKFVRTSRAKTKIKHWIKIEEQKRSLEIGRRLLESEFRRHGMAPAQM
                     LKSSELAAAARQAGYDTTDELIASVGFGHLPTANVMEKLAASSASLVPEKRATPSTRK
                     AVAGKSDDKGVKVKGARDVLMQLSRCCNPVPGDRILGYITRGRGLTIHAVDCPNLEAL
                     DYDRERLVEVEWDSATPSTHAVKISVMAVDKTGVLANVSSAIAECRANISRAEIATRE
                     DRKAVLDFIVEISGTDHLDQVLKAIERVDGVITARRVRAWQEK"
     gene            complement(24201..25904)
                     /gene="recJ"
                     /locus_tag="VFH05_09935"
     CDS             complement(24201..25904)
                     /gene="recJ"
                     /locus_tag="VFH05_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET6678579.1"
                     /translation="MKSKLWVFRDINPSNRAALAQALSISSATASLLLARGVTTPDQA
                     TTWMSHQFPHDPFLIPDMDQAVDRLHRAVVAHEPVCFYGDYDVDGMSATAVYLSFFGS
                     LGANVRAYIPHRKREGYGLNAKAVQWLHDEGISLLVTSDCGTTSHREVALAAQLGMDV
                     IVTDHHQTDEELPPAVAVMNPHRADAQYPFLGLCSAGLAYKVAQAYQLRYGEAGVPLE
                     SLLDLVALATIADVVPLHDENRGFVREGLKQISRGARCGIRALKQVAGAVRDCTAETI
                     AFKLAPRLNAAGRLDHAMLGVRLLTTESPAEAQQLADRLEQLNRERQKIEAELMAEAV
                     ASLKDQELPAAVVLASRRWHVGVVGIAAARLVEKFHRPAIVIAIDEGGIGKGSARTTG
                     GFDLYQGLAACRDLLEAFGGHPSAAGVTVRESNLEALRERFSAVVAEWNGGETTVPTL
                     QVDAEVQLSEITARLINEIGTLHPFGAGNPEPTFAVKGLEVMNARTVGEKHLKMTVRQ
                     GRSMPFDSIGFGMKSLIERGIPAGTPVDLAFLPEFNHWNGYDRIQLRIRDLRVSDRGT
                     A"
     gene            complement(26067..26264)
                     /locus_tag="VFH05_09940"
     CDS             complement(26067..26264)
                     /locus_tag="VFH05_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HET6678580.1"
                     /translation="MEKQERKQEVRREPQGKEEVKANPKVVETGKKLKEDIDKLVDEI
                     DDVLEKNAEEFVKNYVQKGGE"
     gene            complement(26403..28460)
                     /locus_tag="VFH05_09945"
     CDS             complement(26403..28460)
                     /locus_tag="VFH05_09945"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET6678581.1"
                     /translation="MSDSAIVTFALVAACAGIGYGLYLAMWVFRLDAGNAKMQEIAKA
                     IQEGAGAYMNRQYKTVGVVAAVLFVVLAAAGAVSDKFGILTAVGFLVGAGASAIAGYV
                     GMIIAVRANVRTAQAAHQGMNAALTVAFRGGAVTGLLLIGLGLLAITGFFMIAQSISG
                     QEKAVHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAYTMFKGASAPILYPLALGGVTIFAT
                     IIGIFFVKVGPGGEIMPALYKGLFVAGGIAAIAFYPVTTQVMEGIGGVSGMNYYIAAL
                     IGLVVTLALVFITDYYTSKSYAPVQYIAKASETGHATNIIAGLAVGMQATAAPVMVIA
                     SAILGSFWICGGVESGGLYGVAVAAVSMLSMAGIVVAIDAFGPITDNAGGIAEMSHLG
                     KAVRDITDPLDAVGNTTKAVTKGYAIGSAGLAAVVLFAEYSREVAAHNAALAAFDLSN
                     PKVLVGLFLGGMLPFIFGALCMKAVGEAGGLIVEEVRRQFRTIKGIMEGTGKPDYATC
                     VDIVTQAAIQKMMVPGLIPVLSPVIVGVVLGPQALGGVLVGSIVTGLFVAISMTSGGG
                     AWDNAKKFIEEQGKKGSETHKAAVTGDTVGDPYKDTAGPAVNPMIKVINIVALLIVSL
                     IVK"
     gene            28793..29146
                     /locus_tag="VFH05_09950"
     CDS             28793..29146
                     /locus_tag="VFH05_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678582.1"
                     /translation="MVSLLLAATPAVAELLEVADVIARPQHYDRKEVVVMGQVSGIQA
                     ITDKQGQPAFQFLLKDNIGTIKVISRREVLDGDQIIVEGTFTRRRQGGRLAVYNEVNA
                     IVIRPLNQFDADLVG"
     gene            29282..29593
                     /locus_tag="VFH05_09955"
     CDS             29282..29593
                     /locus_tag="VFH05_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678583.1"
                     /translation="MKSIMLSFLAAGLLCFGIGVAQAANPMSEKEASPTLKERITKDT
                     VKGTLMKTEGEYYWIKDTDGKEVKIHVDKSTKMDKVVVGDMVKAYITDKGHTTTLQRA
                     D"
     gene            29680..29862
                     /locus_tag="VFH05_09960"
     CDS             29680..29862
                     /locus_tag="VFH05_09960"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET6678584.1"
                     /translation="MARYGKAAQKSVKRTMHKRKKGTLTSGKSKKRVKNRKQAIAIGL
                     SEARRKGAKVPRKKAA"
     gene            29983..30360
                     /locus_tag="VFH05_09965"
     CDS             29983..30360
                     /locus_tag="VFH05_09965"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET6678585.1"
                     /translation="MGAPHRNATDTFHHFARQLAEAVGSPWAFLLALIVVIVWLASGS
                     TFHYSDTWQLVINTGTTIVTFLMVFLIQNTQNRDAKAIHLKLDELIRGVAGARNRLID
                     LEELSEAELDDLQRQFAKFRRQR"
     gene            30434..30823
                     /locus_tag="VFH05_09970"
     CDS             30434..30823
                     /locus_tag="VFH05_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678586.1"
                     /translation="MPNTNASFSPRMRSDELKEQIGKLERLEHRTPVLPTLDSFDEET
                     EALLGRLYGDSHKYLEAYKYATVAESEALVNLPESAQEPMAKDLPKTAVQQRRQVLLG
                     ILTEMQDMEANEVAMLTGEDREDPPSI"
     gene            30898..31146
                     /locus_tag="VFH05_09975"
     CDS             30898..31146
                     /locus_tag="VFH05_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678587.1"
                     /translation="MEICTMLGEVNMKNLRKEVRELISINEKIQSALLHGDRMTDDEA
                     TLIRQCASELLEKIPPPSRTWIRAGRTEEGGEAVAGNP"
     gene            31390..31950
                     /locus_tag="VFH05_09980"
     CDS             31390..31950
                     /locus_tag="VFH05_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6678588.1"
                     /translation="MKNAAGFILIGTLGLGSMLMQACASQSGSVGGDEFAQEQITEPM
                     IKHIPPPEYSMNTSRRPSQRAELSAVNATGLPFGALSDVLFDFDQAVLRRDALPLVEA
                     NARRLVQDGVTRLLLEGRGDEVGTSAYNIVLGERRARSVKSYLQERGLSLQLKTTSYG
                     KDRPLCYQHNEECMQKNRSVHFLVKD"
BASE COUNT         7042 a         9505 c         9002 g         6737 t
ORIGIN      
        1 ccggtcgtgg gattacggaa actgtatgac tgaaagttgt tgccgggatt atagagaaat
       61 ccctgcgtgc cctgattgtc cagatagaga cttccgggac cggtgaggct gaagagcggc
      121 gcgacgccgc cgtcaaacga acgtacactc gaaatagttt gcgccgaagc cggctgctcg
      181 agaccgcaga accaaagcac aaccgagaca gcaagcacta accccaagcg ccttggaaga
      241 agtccatgac agatgtccat gaagcgatta tagtatggga ccacgaagct tgtcgaccaa
      301 aggaggttct atgagatctc gactcgctgc cggcctccct ggtgttctgc tgctctctgc
      361 cgcgagcacg ctggcaatga ccgccggacc tgctcttggt gccgatgacg tcacgaaggc
      421 tacacccggc gagcggcggg aagagatttc cctttcggaa gccgccctcc gggcactcaa
      481 aaacaatctg gacatcagta tcagccgcca cacgaaggag agccgcctga ccgacatcgt
      541 tgtcgaacag tcgaaattcg accctacgct cagcgtcaac ggacaataca accggaccgt
      601 caacccgctc aaccggcccg tcttcggcgg aaccggcggc agtctgaacg agattacgat
      661 cttcgatcaa cgcaacggct ccgtgaccgt ggacgccacg acgaacctct tgaccggcgg
      721 caacgtcgat ctgaattaca gtcccgctcg tacaaacgtc aatcaggacg tggcgagcgg
      781 cttcttgttc aatccggcct ggaccggcgg tctcgcgctc acgctgacgc agccgttgct
      841 gcgcaatgcc gggatcgaca tcaacaagac cttcatccgg atcgcccaga acaacgctat
      901 ggtcgaagag catgtgtttc gcgaccgtgt gctgactgtc gtcgcgaccg tcgagcagac
      961 atattgggaa gtcgtctttg ccaacgagaa cctaaaagtg gcggaagcgg cactgaaagc
     1021 cgcacaggaa ttgcttgcca ccaacagggc gaaggccaag gcgggcgtga tgtcgatcgt
     1081 ggacgtgctt caggcagaag cggctgtcgc atcgcgtgtc gaacaagtgc tggtcgccga
     1141 aaagtcaatc cgcgatcagg aagacctgtt gcgccggctg ctgaatccgg gggaggagga
     1201 gttgcgccaa gacgtgcgat tgaccccgaa ggaccatccg gttgtcgctt tggagccgat
     1261 cagtctgccc gaagccatcg atctggccat cgaccttcga ccggagatcg tccaggcgaa
     1321 gaaaaatctg gagaccagcg aactcaacac caaattcgcc aagaaccaac tgcttcctac
     1381 cctctcgttc cagggcacga tgggcctggc cggtcttgga aaggactata ccgattctgt
     1441 caaccgcaat ttcagcggtg acttctataa ctatggcgcc gggctcgtgt tgagctatcc
     1501 cctcggcaac cgctcggcat ggagcacatt caacagacgg cagatcgagg gcaagaacgc
     1561 agagacatca ttggtgagca tcaggcaaca gatcatcatt ggcgtgaaag aagccgtccg
     1621 ccgggtccaa accgatttca aacgcattga aacgaccaag tcggcgcgga ttatggcgga
     1681 gaagcagctg caagccgagc aagaacgcct gaaagtcggg ctaagcacca cgcgtttcgt
     1741 gctcgatttt caacgggacc ttgcgaccgc tcagggcaat gaattgcgtg ccattgttga
     1801 ttacaacaaa tcattgtcca atctgtcccg ccataaagcc acgacgctgg agcgctacag
     1861 cttacaggtc caataacgag cgatgacgcc gcctggtcgt ctcggtatct ccgtccaggc
     1921 aaacgagcgc ggtgcactgt tggcgctcct gccgattgcc gcgaccatcg attattatgc
     1981 cctgcccgat tcgcttcaag aacaagtcct gatccaattt gctcctcaac tcatcggtta
     2041 cctcgccctg gttttatggg cagtgcacaa tgattccgtc ctaagaagac tcgggttgga
     2101 gagggggcta tggaaccgcg gagcgaaact gggcatcgtg accggcctgc tccttggctg
     2161 cctcaattcg ttggtgatcc ttcaggtcct gccgtggttg ggatgggaca tcaccttctt
     2221 gacgcagacg cctcacgccc gggtgccggt aatggtcatg gtgccctggt gcattgctgt
     2281 gatcgcgatc gtcgtcgaga tcaacttcag gggttttctg ttaggccggc tggcggcact
     2341 cgaatccggc ctctggcctc aacctgctct taagcagctt tcgcccgcgg ccctattgac
     2401 cagcgcattg gtcttcgcta ccgatccgtt catggtcaac accttcaaac agctgcactg
     2461 gatcgcgctc tgggacggcc tgatatgggc cgtcctctgg atccggacga gaaacctgtt
     2521 tgcaacgatc gtcgcccatg cgattgaagt catcatagta tatagtgcag tgcgaaccgt
     2581 gctgatgtca tagtcatgac gccttcattt tccagctacc tggtgaacga ctgcttcggc
     2641 gacccgggcg tctatgtaga ggtccgatgg tcgaagcgcg cactgctgtt cgatttaggg
     2701 cagaacgacg cgctcggccc cacccgcctg ctgcgtgcga acgacatttt catttctcat
     2761 acgcacatgg accacttcat cggattcgac gccgtggttc gtgtcgcgct gggtcgtggc
     2821 aagaagctcc gcctgtatgg cccccccgga ttgatcgaca acgtgcacgg caagctgcgc
     2881 gggtacacct ggaatctggt ggatgggtat ccgctgatta tcgaagttcg ggaatttgac
     2941 cacgcagaag ttcggcaggc gacgtttcac gccgcgaacg gcttcatgct ctcgacgccg
     3001 acgattcatc cgctcgtggt tcatccggac accggccttt ttactgtgct cgaagatccg
     3061 atgtttaccg tcaaggcggt cgccttgaac catcgcattc cgtccttcgc ctatgcgttg
     3121 gaggaacagt tccacatcaa cgtgaacaaa caaaagctgc acgaagcggg attgccggtg
     3181 ggcggatggc taagagacgt caagcagtat gtgtgggcag gaaagccgga tgactttcgg
     3241 ttcaccgctc tcctgttcga cgaacatcgt caaatggaac aggagtttgt gctgggggac
     3301 gtcaggaacc gcttcctgac catctcgcgc gggcaaaaac tggcatatgt cgtcgacgca
     3361 cggttcgacg aggccaatga ggccaggatc gtcgcgctcg ccgcgcatgc agacctgttt
     3421 tattgtgagt caccgtacct ggatgccgat gcggaaaagg cgagggaccg ttatcacttg
     3481 acggcaaggc aggcgggcat catggcgcgc aaggcgcaag tccgggatct catcgtgttt
     3541 catttctcac cacgatatac aggtcaaggt gaggccatcg aacaagaagc tcacgacgcg
     3601 tttctaggtc actgaggagc gacacgtgaa cgaattagga aaatacgccg tgtactttct
     3661 attaggaggc accattgtca gtgtctcgac ctatttgggc gctcagggca ggccgtacct
     3721 tgccgccttt gcgagcacgt ttccggccat caccggcgcc acctttattc tgctctatgt
     3781 caacggcggc agcgatgcca tcgtcgccta tgcagagaag ctgttctggt tcgtcccgcc
     3841 ctggatcatc tatgttcttg ccatgatctt catcgtcccg cggcttggct tctggcctgc
     3901 catgactctc tccctggtgc tctacatggg ttgcgtctgg ctggtcagac tggccgtacg
     3961 ctaactccgt ccgaagcagg gattcgccct tgaagatggg aacttaccgc ggggaagggg
     4021 cgttgtcacg aatacccctc cataggtcta ccctctttga atactaccgg acaggccctg
     4081 ccggcgatag agttactaca tgctttcaac tctccacaag gaccgtccgg ccgggaggcg
     4141 ccatgatcag tcttgttgag tttgtcgcga tttgtagcgt ggcgacctta accgctgccg
     4201 gcgcatccca agggtgggac cgcttgcggc gaccgagggt caaccgctct accgatcgtg
     4261 agaaatctcg cgacgatgga tccggacgac catccgatgg ggcatggcac gaacctcgct
     4321 atggcaagcg acacgcagtc cggtgcgcga tcgagtacca ggtgggagca cagaagcaag
     4381 agggcatact gatcgatgtg tcgcgccggg gttggcgggc aacgggtcct tcgccggttc
     4441 tcgctggcac cagcctatcc gtgcgggttt ttttgccgga caccgctcag tcggtcttca
     4501 tcgaggaagc agtggtgcgt tggtccgatg gcctggactt cggagtagaa ctgatgcgta
     4561 tcagccctga ggacgggcaa gtgctcagcg agtatctgtc cgttcactat ccaatggaac
     4621 cctccccttc gcacgccttt tcgccatttt cctacaacta ggctgagagc gcagctcatc
     4681 gttgctgatg caactgatcc aaacgatgtg cgattgccga gtaggcctaa gggagagaaa
     4741 tcgagtcagg cgaatcaggc tgcactaccg aaatgcagct tactcatttc cgtcatggct
     4801 gcgaccagct gatcaaccgc ttcggtgaca caagtactgg ccttgtggga gggacctttc
     4861 ccgccggccc agatatccgg cggatgatcg atctcaagaa cattttgaca aaattgggtg
     4921 agggaccgct tccaaaacac tccgtcactc ccctcgtcga ggaaatgctc gcgatcctgt
     4981 gtaaagggct gatcaaactg cttggctcgc atttgccaca tccgttccat cgcattctgc
     5041 cgttcctgac tgaattcgag accggtcgca cgctcgttca acgcggcaaa caggccacca
     5101 atcacccgtt cgacctcggg gctgggaaac atccccacac aggtttgcac gatcaagtat
     5161 ttgtggaaga ggaacacttc agcgctgatt cgtgcctcat tggctgaggg atcatacgcg
     5221 cgcgcgagtt cctttaaggc ctcaccggaa ggtccttcca agctcgagcc cagcaatctc
     5281 gcaagctggc gtccgattcc tagatagtcg gtcggcacga tcggctgggt catacaagag
     5341 tgattccttt aatgacgagg gtaagcttag aggattgcat ttgagcctgt ccatcctctc
     5401 acccagccca agaatcccca agacaaaact acaattcgcg ccgtgctaag cggtccactc
     5461 tcatggacag acgacgctgg caggaaccag cgcgcgcctg ctaggatgcc gggtcatgct
     5521 ccgaagggaa ttcgacgtgt ctcaccttcc agacgcagaa tcggcgtcac aagcgctcat
     5581 cgttgaagga gcacggcaaa acaatctgaa aaatatctcc gtcctcatcc cgcataatca
     5641 ggtgaccgcc atcaccggac tctccggttc tgggaaatcc tcactggcgt tcgatacgtt
     5701 gtttgccgaa ggccaatggc gctacgtgga gtcgctctcc tcgtatgcca ggatgtttct
     5761 cgacaaggtt gcccggcccg atgtcgatcg tgttctgaac atccgtccgg ctattgccat
     5821 tgaacaaaaa aaccaggtac ggaccgcccg ctcgacggtg ggcacgacga cagaaatcgc
     5881 ggacttgctt cgcctgctct ttgccaaaat cggaaagccg atctgtcctg actgtcatga
     5941 agaggctcgg tcatttcaac cggataccgt cacgagcgat ttgctcgaac ggtttgcgga
     6001 agcgaaggct atgctgttat tcccggtgac gccgccttcc gcaaagtcgg aatccgcttt
     6061 tgtgcaatcc ctcctcaccc gtgggttcac cagactgaga atgggtcatg aaaccattga
     6121 ccttcatcag gatgcactcc ccccctcggt gcatgggcag tcgtctctgt ccgtcgtcct
     6181 cgaccgcgtc gtgattcgag aagacaaccg gggacgtctc tgtgaggcga tcgaaatggc
     6241 gtttcgtgaa ggagacggcc gctgtgacgt ggaggtcatc ggccacggat tgcaacagta
     6301 ccatgcaggc ttttgctgcc agcgatgcgg acgcaccttc gaaccgctca ggccgattct
     6361 cttttccttc aatcacccac tgggcgcctg cccggattgc aaagggttcg gcaacgtgct
     6421 gcgatacgat cccgcgctgg tcattcccga tcacagcaaa tcgttggtgc aaggcgccat
     6481 cgagccatgg agcaaacctg gaaccgactg gtggcaaaaa cagatgctcc ttgcgatgaa
     6541 acgaaaaggc gtggacatca cacgtccttt caagttgctg gacgaagacg tcaaaacgct
     6601 gttatggaaa ggcgataaga cattcgacgg aatcgatgac ttctttcagt atctggaggg
     6661 caaacgttac aaacttcatg tgcgcgttct tctcagccgc tacaggacgc cgttcgattg
     6721 ccccggctgt cacgggagcc ggctcaagcc tactgcccgg ttcgtcaaaa tcggcggggt
     6781 cgacatccat gacgtcaccg caatgaccat cgaacacctc atggcgtggc tgcaaggcct
     6841 ggccctacgg ccgtatgagc gaggcattgc taccgatctt ttacggcagc tcaccgcgaa
     6901 gttgggcttt ctcatccggg tcggtctcgg ctacctgact ttagggcgac agacaaaaac
     6961 gctgtcgggc ggcgaagctc agcgtgtatc gttggcgaat caattgggcg ctcacctggt
     7021 ggggacactg tatgtgctcg atgaaccgac gattggcctc catgcccgcg atacggattt
     7081 gctggccggg attctgaagg acctggccgc ggccggcaat acggtggtcg tcgtcgaaca
     7141 tgaccggcag atgatcgaat ccgcggatca tattatcgag ctggggccgc actccggtga
     7201 aaagggcggt gaagttgtct gcgccgcgcc ggccggcgag tttctccgtg atcgccgtgc
     7261 gatgaccgcc cggtatctca ggggggaaga acacattccc ctcccggcct ccaggcgctc
     7321 ggggacgggc aaagtccttg tgatcgccgg cgcgagcgag cacaacctga aagacctggt
     7381 cgtgcggttt cccctgggca tgttgatctg cattacaggg gtctccggct ccggcaagag
     7441 cacgctcgtg gaagatacgc tgtatgccgc attggcgcgc gcgtttcgga cgaaaactct
     7501 gccgatgggg cgcttcaagg cgatcagggg gatcgagcat ttgaaaggtg tacgattgat
     7561 cgaccaacaa ccgatgggcc ggacaccccg atccaatccg gtgacctacc tgaaagcctt
     7621 cgacgaaatc agacgccttt ttgccggcga acgtgaggcg ctgcggcagg gtttcacagc
     7681 tggacatttt tctttcaacg ccgggggcgg tcgttgcgag cgctgtgaag gagccggcgt
     7741 cgagaaactg gaaatgtatt ttttcgagga catttatgcg ccgtgcgagc gttgcgaagg
     7801 cagacggttc aagccggaga ttttggccat tcggtatcgg ggcaagacga tctcagaggt
     7861 gctcgcattg acggtcgacg aggcgcgctc gtttttttcc ggaaccccga agctgcagga
     7921 acggctgcat ctgctgtcgt ccatcggtct gggctatctg cggctcggcc aaccggccac
     7981 cacactgtcc ggcggcgaag cccagcggtt gaaaatcgcg gccgagttga aagatgccgg
     8041 cgcaaaggat ctcctctaca tcatggatga gcccacgacc ggtctccaca tggatgatgt
     8101 aaaaaagctg ctcgtcgtac tgcaaaagtt ggtgaatgca ggcaatacgc tcgtcgtggt
     8161 tgaacacaat ctggatgtga ttaagtcggc tgactgggtc atagacctgg gaccggaagg
     8221 gggccaggca ggcggtcaca tcgtcgctga aggcagacct gaactcatcg caaagtgcga
     8281 tgcgtcatac acggggcgat gcttggctca aaccatgacg gaagccgttg accgatttat
     8341 ccttgagccg cacccgattg cgtaggaggc tgctcgcgcg acagcttcct gtgtaacaat
     8401 ttcttgctga acgtcgtgat gccgaaggcc aagccaatgg cgatcggaac aaaaatcgcc
     8461 gaggtcagat tggcgttgcg caaccatcct atttcttgga gacccttgaa aatgtagcca
     8521 ccgagcccgg cgacataata ggcaatcacg ataaccgaca atccttccac cgtatgttgc
     8581 aacaagacct ggctcttcgt agttttatcg acgctctgga gcagcgccag attctgcgct
     8641 tccagaatta aatcgacgcg ggcccgtatg atcgaaatga tgccttcaaa accggctcgc
     8701 agcgtgtcga tgcgcttcag caactgttga tagccttcag caacacccgt gacgccgctc
     8761 aacacgtaat cggagacggg tcgataggcg tccagcgcct tctcgttcaa tgatgccagg
     8821 gtggtatgca caatcttatc gtacggcacc gaagctgaca gctcgaagtg gagcttcccc
     8881 gcgatccggt tcgtcttcag caagtcctga gtgaggctat tgagccagcg ttggagcgtc
     8941 tgcggatcgg cgtgcgcgat gtgcccgctg ataatctcgc gttgtttcaa atggacctgc
     9001 tcaaacttat acacctgatc gatcgcggcc gagaacaacg gcttttgcat gagcaacaga
     9061 tggtaatagg tttcaatgcg gacgatcgcg tcgacgattt ctttcaggcg agacgcctgc
     9121 gcccgcgtcg ggccgagggc cacccagtag cgctcccgtt cgaattcgtc atgggtaaaa
     9181 ctggtgacga ggctggtctg ttcatcgaga attctactcc catacaacac agggcctggc
     9241 atcatcgacc gcatctgttc tcgcggcggc agggaatcgg atgacagcag gatgtccaat
     9301 cggcagactt ccgttccgag cggcgagatg ggaaaggtat actccgggaa ggtcaacggc
     9361 ccgaatgcga tctctctgtg gacaccgtcc ggcaaatgcc agatttgata gttgtaatat
     9421 tccgtatgcg cctgccagat gataatcaac cgttcgccgt tgccggcttg tttgactccg
     9481 tacccgaacg tctcgtgtag cacggtctca tccttcggga cgccgaacgc cgtaaggcag
     9541 gattgaaact cctccctgct cgcctttcgg tccgccggcg gatcggccat gcgaaatgcc
     9601 ttgtagtgga cgtgcgcggg cgcacggagc cagtcggcga gcgggatgtg cggccgctca
     9661 tgcaatttct tgatcaacgc atcggcaccc gattggcctc gtgcgcgctc catgcgattc
     9721 ctctcctacc ttctggaggt tacaagcccg gtcgcttctc ggccgttacc gacaatcgca
     9781 ccggatggtt cgcccggctg acatccaact gcaatgtgcc cccggcttgt acctggcaaa
     9841 gatgtttggc ttcacggtcg tttctggctc cggagagcga agcgccgttg cacgccacaa
     9901 tgtggtcgcc gactttaagt ccggcccgtt cagcaggaag cccgctccgg agttcccgta
     9961 ccagataaaa ccgcgctccc tggtcctcca gtaactccca attcacgccg atacgtcctg
    10021 aacccagcaa gccggtctgg gaaccaaaac gggcgagaat acgatggacg atgagacggg
    10081 ccgaaccctc gggatcgtcg gttgtaaaat cagtcaggtg gccctcgccg ttgaacagca
    10141 actgccctgt ctcgacgtcg atcattcgaa acgaaaggga caccttgttc atgcggctct
    10201 gctcatggcg ttcccattct tgcacgccgc ctacgatgat ggcctgtgcg cctacgagtt
    10261 ttccaacctt gagcacgttt gcatcgtccg catgggttag ttgaatgacc tgttctttca
    10321 acacttcgtc gagttttgcc cgctccacca cgttgatgtc gagatcgagc aacaacgtcg
    10381 tgacgatgcc cgccacgcga gagccggatc ctgctacgcc cggcgcatct tccaacaaca
    10441 tcaccgccat ggtctgatag cggtcgacgg tgtctcgatt gatggggccg aggcctgtca
    10501 cgacaggctc gggcctcccc gcagctcccg gcggccggcc cgggtgcatc ccggtcgatt
    10561 cgcaggctct ccggcgctga aggatcgctg agggcgtgcc ccacgcgcat agctcttcat
    10621 tcacgagcac gacatgatgc ggatactccc tctcatgttc catggcgaca ttggtaaaag
    10681 gatagatgat gcatccgccg atccagaccg aaaccggaca gagaccgagt tcgtaaagcc
    10741 attgccggcg tgccgtcaaa gaatattccc acaccagcat gcggccgtca tcacgaagca
    10801 cccgatccgg ccggccgatt tcgtcgatga cctgccgttt cgtcatgggc acggacaggt
    10861 tgtcgagctt cgattcagat ttgacgatgg tgtacgcgag gccgcagcca aaaaacaaag
    10921 tgggcacgat cagcgcaaga atgaaggtgc aggagcccgt gaaccgaaac cacgcttctt
    10981 ccggtggccg cgacatcgcg tggagagtag cactccgtac gcacggccgc aatggatgca
    11041 tcccgcgact gtagccggat cactttggag gccaacgctt gcgttgaacc gctggtgtgg
    11101 aatggatgga gacggtgctg gcgcccagta cgtggacgcc gcctcctttg cgggcacggt
    11161 gtgtacagga cccgttcagt tgagggaggg gtcgaagggc atatcggcgt acactcgaaa
    11221 ctcctcgccc aacgtggtga ggatttcgga ccacacgcga gtgggatctt tctcaaagac
    11281 cgtggcggga tcagccggaa cggtgatcca agacccgatt ttcatctcag actcgagctg
    11341 ccccggaccc cagccggaat agccgacgta ggcgcggaag gcttctttgc catgagtggt
    11401 tgtgagaatc ttatctacca attccatatc cccccccaaa cagacgccgt caaagacatg
    11461 gtgggaattg tcaggcaatt gatccaatcg gtacaacagc atgacctgat tgggctgcac
    11521 gggcccaccg gaaaacaaga catgacgctg tccttccaac accggcatct gtggcaaggc
    11581 ttcggaaacc gacatcgccg tcggccggtt cacgaccacg ccaagggcac cttccgctcc
    11641 atgctcacag aggaggacca cggtctgtct gaagttggga tcgcggagat tcggcgcggc
    11701 tacaagaaag atgcctttgc cgagttgcgc cctcaacgga atctgcatga gaaatatcct
    11761 atcctgccga tgcgccacac gcaacccagc aaaaggcagg gcctgtaaac ttaccggtaa
    11821 aacgtctcgc ggcggtcctt gagcaaatcg ttgtatgggg tgagtgattt attgcgggct
    11881 tcagccggat cgatatcgac cacacacagc gcttcctgat cacgcggcgc gcgctggaga
    11941 atagcgcccc gcggagaaac cacttcgctg ttgccgatat aggtaagtcg gtccttgcct
    12001 ccgcgcgcct cgctgccggt tcgattacat gtcacggcaa acacgcgatt ctcgagacaa
    12061 cggaccggca tggaatccgg gcagttcggc agcacgagat tggaaggatg acagatgatg
    12121 tccgcgcctt tgagcgccaa ggcccttgcg gcctcgggat agtaccagtc gaaacaaatc
    12181 atgacgccga tcttggcagg tccgatatcc cagacatgaa agcccgtgtc tcccggcgtg
    12241 aaatacagcg tctcctcgaa aaagagatgc gtcttacggt agctcccgat aaagccctgc
    12301 ggagcaacaa cgacagccga gttgtaatac gctgcgcctg cctgctcggg cacgccggcg
    12361 acgatcacca tctgtctgcg cttggcgaga tccagcaggc gcttcgtcgt ctgtccggcg
    12421 ggaatcggct cggccagtgc gcgcacttcg tccatcgaga caaattgata cccagaggcg
    12481 cacagctcag gcaggacgag cagatcggct tcggcgtctt cgagctttgc cgcaatgcga
    12541 tccacattct cggcaacctt cccgaattcc ggattgtttt ggtagtaccc gacccgcatt
    12601 gggtgctccg attcacgcga gggtgtcaag acgtggaaga cgatgcttct cagggatgcg
    12661 aggatgaatc cgacaagctg ttccccatcc tgtacggctt acagacgaca acgggcggga
    12721 catcatcatc cccgcccgtc atcagtacag cacacgtagc tgaaaaacgt tacttcactt
    12781 cggacagatg cgtcaccgcg ccttccgcat gctcggtcgc ctggtcggcg tgaccggcct
    12841 tgccgtgggt gacggcttcc tttaagtgct tgatgccctc atccaaatga ggattcttga
    12901 cgccggcggc ctgcgcgtgt ttcaacgctt cttccgaatg ctgcgccaca gcatcggcat
    12961 ggccctgctt gccatgattg acagcgctct tcgcatgttc aagggcttca cttacgtgtt
    13021 tattgtcggc ttgagccacc agcggtaggc ccagcagtgc gaccgcagcc attgccgccg
    13081 ctccacgtcg gatgctcatc gtcatgggtt cctcctcatg gtttgagtgc caagtcgtga
    13141 tagctgaaga aatcgtacgt agcgaagtca ccttacacag ccactttttc actgtcaaga
    13201 aggccgggcc ggcgcctcta tccccaacac tttctgcagg ccttgaaagt ccttctcaac
    13261 cgggcaggca aaatcgcggc tccattccca ccaggaaccg gggtaattcc tgaccttctg
    13321 gaatccggcc agcttcaaga caaaatacag ccacgaagac cgtaccccgc ccgtgcaata
    13381 acagacgatg tcatggtcgg gatccaaccc tttctcttcg agagcggctt tgatggctgt
    13441 gaggtctttc acagtggcat tggtattcaa gaacccgttc caggaaagat gaatcgcgga
    13501 aggaatatgg ccaggacgag ggatgccgga tacttctttt ccgagatact cttcgagact
    13561 cctcgcgtcc agaatcttca tgtcaggcgg aggatgcttg accatcgccc gcagctcgtc
    13621 tttaccgatg atcgtgtgag tggccggctt tactgaaaag tttccaggct tcggggtcac
    13681 ccgaccatgt tcgaacggcc gctgctccgt tatccacttg acccagccgc catctagaat
    13741 gcgcatgttc ctatgaccaa aatattcaag catccagaac attctgcctt cgtcgcccca
    13801 gttgtcgaac gggttcgagt agatgacgac ttcgctgtcc tcgctcagcc caagcgcgcg
    13861 cattttttgc tccatccggt tcatatcggg atcaagcaag cctttggcca ctgcattggg
    13921 gtcactatat tggtgccagg tcgtatgcac tgctccgggg atgtggccgc catattcata
    13981 cgcggcgttg ccacgcacgt cgatgacgac aagcttcggt ttccccaact gcttctgcaa
    14041 tgtctcggta tcaataagta acggatggtt catgagactc cttcaacccc gcccgacagt
    14101 atcaaccgcg cagcttgacc catgccgcga actgcccgcg acgggcagcc ggacgttcgc
    14161 atccagggcc gccttcaatt gatcggttaa tggaaattcc ctctcgtaga tgacaaagcg
    14221 gtagggatca ttccccgtca gaccatacgt tcgttgcagc atctcatgct gcttatccgt
    14281 cacaatgtgg tactgcacgc gcgtcttgag tttcaagccc tccgccttgt ctggcacctg
    14341 atacgcaaac ggatactccc ggctcgccaa cggcagaagg cggttatcat acaactccag
    14401 gatggccggc tgccacatga tccaacgtcc catcgtcgat gtttcttcct tgagcacatg
    14461 acccagccta tcctcgaccg agaactcgac ggtgaaaaac cgatccggat cacccgtcgg
    14521 aattttatgt cccgcgccgg cattgaccag cgtcaaggtg aaggcaacgc gatcgcccgg
    14581 cttgggcgac ggagggtccg ccttcacttc gacggccact gcccgcttga tcatgtccgg
    14641 atcgtggccg ccgcgccaaa ggtgctgcct accgcgtcgg atgggactgt tggttgcgac
    14701 aggcctgtcg atctccggca tgtgacagct ctggcagata aatccgcgct cctgcatgaa
    14761 gaatttccct tcatactccg cataagtccc gcaaggaccg acccggtaga actgcgcagg
    14821 gccggagacg acattatggc agcgataaca aagttgtgcg gtccgaaaat tcggatcaaa
    14881 ttgggtcggg tgcggcgcgg cggaatcctc gaacgggccg taaatgatgc cctcccgcac
    14941 gtggcaggca gcgcaggtga ccgattcctt ctgatactcc gcgtcgtaat gcgggttcgg
    15001 ctcctgaacc gctttctcga ctcggccgcg gggaatgtct gtaatgagag tcggctgctg
    15061 gttttccaac ggtgcatgac aattcaagca gacccaaaca ttcttatcct ttttccaata
    15121 cgcttggaag tagggatcct catacgcgcg agcgtgaatg ctggatttcc attcatcata
    15181 aatttcgcgg tggcattggc cgcaggactc cgccttgagg cttgtcagtc cctccggaac
    15241 cctttggaag ggaatggcat gggcataatc cgagcgcagc ccaaaaatga cgaccggctt
    15301 gatctccgtg taatacaggt acgtcgcgcc gaacacgacc agtagcccga ggactatgaa
    15361 cttccaacga ctcatgcggc aagcaacgct ttcaaatgag catccacgga cgccgcaaga
    15421 gccgtgagat tgtagccgcc ctcaaggcag gaaaggatgc ggccttgagc atggcgtttc
    15481 gccacgccgg ccaccaaacc ggtcaaatct gcgtagcctg cttcggtcaa ccccatcccg
    15541 gccagcggat cgtcgcgatg ggcatcgaag ccggctgaga tcatgacaaa gtcaggcttg
    15601 aactcctcag ccgcggggag cagggcctgc aagaacgtgg cacgatactc gtcgtcgcct
    15661 tcgcccgcct ccatcggcac attgattgtg aagccttccc ccgcgccggt cccgtgctcg
    15721 gacgcccgac cggtacccgg ataatgcgga tactgatggg tgctgaagaa gagaatggaa
    15781 ggatcctgct caaagctatg ctgggtgccg tttccatgat gaacatccca gtcgacgaca
    15841 aggacgcgcc tgagcccata tttgttctgc aggtagcggg cggcgatggc cacgttgttg
    15901 aacaggcaga atcccatcgc ccggtcggct tccgcatgat ggccgggagg ccgaacggcg
    15961 caaaacacat gctccacctg cccctgcatg atcgcatcga ctcccgcaag tgcaccgccg
    16021 gccgccagat aggcggcagg caaggaaccg ggtgacatcg acgtatccgg atcgagtgca
    16081 acccgcccgc tggtgggggc atgtcgattc aacgcggcaa gataaccggg gctatggatt
    16141 tgtgtaatcc attcgtcctc ggcttttcgc ggttcgatcc gggtcacgcg cgccgctgtg
    16201 ccactctgct ccaactgttg catgatcgca cgcagacgat tcggggactc cggatggccg
    16261 gcgcccatgt cgtgttcaag ataggccgga tggtacacaa gaccggttcg cataatctca
    16321 tcatccacgc aagacgctcc gctcgctttg atcacaggcg agcggatagg cgcttgatct
    16381 ccggcctcgg ctcgttgtgg ttcagcgatg cgagcacgaa gctggcagat ttccagcgtc
    16441 ttggaatgta gcatgcagga atcggcatag acaacgcgcg tacagcgctg ctatcgtcta
    16501 ccctgaaagg aacacatgcc taatccgaga attgaaccgc tcaaaagagt cctggggatg
    16561 gatccgaatg acgaagtggc gtggttcgga ttggggaaag cctacatgga cgacggaaat
    16621 ttcgaagaag ctgccaaggc acttcgacaa tgcatcatcg tgaagccgac ttactcggcg
    16681 gcctactatg cgcttgccca atcgctgcac aagctgaacc tcgttgacga atgccgaacg
    16741 gtctgcggca caggaatcga agtgtcgacg aagaacggcg atgccatggt gaccaagaat
    16801 ttggagcagt tgaggaactc gcttcaaagc agctgacaaa aaccttcctg ctggcgttcg
    16861 gtcagggggg cagtggcagc cggattctgg atgaggatcc gcacacgggg aaagccccgt
    16921 tgttgtttcg accgttcgac cagctccacg ttggccagtc tcgtcccagc ctgggcaggc
    16981 attggctcga catcccaaag tgagatcatc acagcgctga gcatgcgcag tttcgtgagc
    17041 aggagggtca ctgaccccgc cagggccttc aggtccagcc acaacacttc tgatcgcacg
    17101 gttcgttcgc cgcgcaaatc ggcgcgagga atggatatac tcaagaccac cgcatcggcg
    17161 tagtccgtga gctgtttggt cttttgctgg atgcgtgcaa gaacgcgatg gatcagcatc
    17221 gcgcccggat ccattccctc tgaccactcg tcatcgggga ggagacgccg tggaatccgc
    17281 tgtcggccgg acgacccaac catggcggtc acttcaacaa aaaaacgctc gcggccaaaa
    17341 cggcattcca agtccgccgt tcgcgcctgc gattcgggaa ggaaagtcac ctttgcgccg
    17401 atacgaatca gctgggtagc caactcgagt tcggcaagtg ccgactcccg aacaccgtaa
    17461 tccccgttgg tgatccggcg gacgagacgt gccaagcgca ggtgactgga ttggtcggcg
    17521 caatgccgat gcagcgtgtt ccaacgctct tccagatcgt gtgccagccc ctgctcttcg
    17581 cgcttccagc caggaagcgg ctcacgatgc ccctcacgca gatgcgagac aagccggaag
    17641 gtcttccgtg ggcgaaaccg tgccatatct agaccggctc gccgccctcg tcgtcatgtg
    17701 gcgcggggcc cggtggttca gacggtgtcg gcgacaattc ctcatagact tcatcgagcc
    17761 accgtccggc acaactcaac acttctcgga ccaacggttc gggctgtcct gtccgtctga
    17821 tcgtccattg tgagacgtat tccagcaagg cgtcgcgctc gaacagccgc ttggattgaa
    17881 cagccaaatc cgtcaattct ccaaaacccg ttttgaggat tgtggcgacg gtctctcgcc
    17941 cgtagcaggt gctcgccgtg acatattgaa cggcgcggtt gatttcgtgg tcgttcatgg
    18001 tatgacctct gaaggatttt cacatcctgc tcaacacgtg tcaacgcgca aggcgggtcg
    18061 actgggtttt tttctgctcg aagtgtctat gcggatggta agatcgtcag ggcatgaccc
    18121 tctgagaagg agcagcgcat ggccgcaatg aagaaaccca gacagacacc tctgagcaac
    18181 ccgccggatc gatggaactt gagcgatttg gtgaacgagc cggttcgtaa gcttgagcaa
    18241 catctggcaa gtctcgaggc agacgtggcg caatttgaag cggcacggga gacgcttcgc
    18301 ccggatatgg cgagcagcga gtttcaatcg atcgtcaaag tcagcgagcg tgtgaccgga
    18361 aacgtttctc ggctcggcgc ctatgcctat ctgtggttct cggaaaacac gaaggacgcg
    18421 aaggcgcgct cgttcaaggc gcaggtcgaa gagcacttga cggtgttgca gaaccgcctc
    18481 ctgttttttg atctctggtg gcagggagtc gatgaacaga acgccgcacg attgatggcc
    18541 gacgcgggcg atgtccggta ccatctggag accatccgcc gttataaacc gcatacattg
    18601 tcggaaccgg aagaaaaagt catcaacgtg aagaacgtga ccggtcgcag tgcggtcaac
    18661 actctgtacg atctcgtcac gaatgggttg accttcactc tgcgcgtcaa cggaaagaaa
    18721 aagctcctca accgcgagga actgatgacc ttcgtacgca gtcacaaggc ttcgatgcgc
    18781 caggccgcgt atcaggaact gtaccgcgtc ttctttgctc aacgagattt gcttgcggag
    18841 atgtacaaga cgctcgtcaa cgactggaaa tccgagaatc tgagcctgcg ccgtttcacg
    18901 tcacccattg cgacacgcaa cctcggcaac gacgtgccgg acggcgccgt ggaggcattg
    18961 ctgtccgtct gcgctgagaa tgccgatctc tttcaacagt acttcaaact gaaagcgagc
    19021 atttgcggca tccgcaacat gagccggtac cacctctatg cccctcaccg gagcgagacc
    19081 aagcgctatc gatacgggga cgccgtgcga atggtgctgg aagcctatcg caaattttca
    19141 cctaagctgg ccgatctcgc ccaacaagtt tttacggatc gccacatcga tgctccgacg
    19201 agatcgggca agctgggcgg agcgtattgc tatagcgtgg tgccaggcct gactccctat
    19261 gtgatgttga actacacggg ggacgcccgc gatatcgcca cgatggcgca tgaacttggc
    19321 catgccatac acgggatgct ggcggcgcac cattccgtct tcaccttcca ttcgacgttg
    19381 ccgctcgctg aaacggcgtc cgtattcggt gaacgcattt tgtccgatgc gctgatgcag
    19441 caggaaacca ataaaaaggt ccgccagggt ctgctattga gccagctcga cgatatttat
    19501 gcgacggtgc ttcgccaggc ctacttcgtt cgattcgaaa agaccgccca tgagatgatc
    19561 gcgggaggtg cgacaggaga ccaacttgca caggcctatt taggggagct gcggcagcaa
    19621 tttggaaagg ccgtgaaagt cccaaatgag ttccaatggg aatggctgac catcccccat
    19681 attttcgcga gtcccttcta ctgctacgcg tacagtttcg gcaatctctt ggtcctagcc
    19741 ctgtatcgca tgtatcagca ggaaggcccg gccttcgtcc cgaagtacct cgatctcctg
    19801 gcgaccggcg gctcccgctc gccggagcgc attctctcgg cggtcaacgt cgacatgacc
    19861 tcaaaacagt tttggcaatc gggttttacg acgattcgtg aaatggttgg acaacttgaa
    19921 gcgacgctct agcgtctccg ggattacgcc cgtctcctaa ctacaggaaa atcaagtact
    19981 ttaagcaaca gatcgaccct gttgacaaga tccatcagac gtggcatact gagaatgctt
    20041 ctcataatcg ttcttggaca gggaggcgcc catgtatctg tgcttatgca aggggatcaa
    20101 ggattcggat gtgcgcgacg caggccgggc cggaatcgtg atgccctgtc agatcaaggc
    20161 aaggttcggg ctgaaagact ccggctgctg cggccggtgc tcgaagaata ttcaagcctt
    20221 cgtcgaactg gctaccagtg aaacctgcgt cccctgcaaa aacaccgtga gcagctaatt
    20281 ttctccacca accttccaat cactcttccc caacgatcat ggctgatggt gcatgcgcga
    20341 accgctgcct cttgagcacg ctctttctga atgaaatgag accgtgggcg aggaaaatac
    20401 actattcgga aggccgtcgc ttcaacatgc cggccacttc ggccacccgc cggccgagcg
    20461 cctgggcgtc cgccaactct ttcttgtcga taccgtgact ctcgccttct gtcgttgcag
    20521 aagcgccgaa cgcgccgccg gcactgacaa tgatcatctg attgcccagc attgcagcga
    20581 ggattgtcag catcgtcact tccttgccgc tcgagatctg gccccccgtc gcaaaggcgg
    20641 cgccgatctt gtttttcatt ctaaactccg ggaagacacc gaacttgaat tgccagttgt
    20701 caaagaaagt tttgacctct ccagacatgt tcgaccagta cacaggggag cccacgacaa
    20761 gggcgtcgga agaaaacaag tcgtctgccg tcacgtgagt gacccgcttc atcacgacat
    20821 ccgtgccgga cacgctcttg gccccttgaa cgacagcttc cgccatgcgc tccgtgtttc
    20881 ccgacaacga atggtaggca accagcacct tgacggcgga aggagccggc tcctccgcac
    20941 ggacgaagcc acatccgaac ggcgagagta atgataggag gcaaagaatc aggcaggaga
    21001 tggagcggat cgcgtcacgc cgattcaccg acgaaacgtt tcctcttcca tgcgttcttc
    21061 gaccgagaca tcggtcagag cgccccggta gtcacagcgg tggcaccgca cacgccagga
    21121 ttcgatccat ttttcctgga cgtaggactg gcggcatttg gggcagacga agacgccctt
    21181 gacatagtgc actttcctca cttctttcat acaaacctcc acggttcgag tgagaatcgc
    21241 acaggtccat gcgggaatgc aaggcgagcc ggcgtgactg gcgtgtcgcc ttgactcggt
    21301 gtcgacgcag tcgataggat gccgcccgtg cacaccgact gggtcattgc tgctgtactt
    21361 gcgctgatcc tggcgcttcc ttcctgcact caagccgacg acggactcat tcccattcag
    21421 cttcccggcg gggtcatcat tcaggcagag atcgccagca cgatgaagaa acgcgccgaa
    21481 ggattgatgt accgcgaaca tttggcaaaa gaccgcggca tgctgtttac cttcgatcag
    21541 gcgcagccct ggactttctg gatgaaaaat acaaaaattc cgctcgatat catttggatg
    21601 aatgagaaaa aacagatcat tcacatcgca cggaatgtcc cgatttgcac gcgcacggat
    21661 gatggctgtc cccagtacca gccgaacgac ccggccacat atgtcttgga acttggcggt
    21721 ggcgaagcgg agcgcctcaa actcgagaag gggacaacgt tgcagttccg aattccgtaa
    21781 gcagacgcat gtgccatccg aaactgagac tattcacagg agcctcccac ccaaccccga
    21841 agccccaaga cgcttccttc cacgagaagc cgcagcgcgc gagggcttga gcgaggcgcg
    21901 aacactcgag ttcagctgcg gtcaggggag atcacaaggc gctcttcgaa ttgggcgggt
    21961 ggggaaaaaa tcgtctgcag tggaagggtg agaagcccag cgggtccttt tagacggccg
    22021 ttcttacttt tcctgccagg ctctgacacg ccgggccgta attacaccgt ccacgcgctc
    22081 gatggctttg agaacctgat caaggtgatc cgtcccggaa atttccacaa taaaatcgag
    22141 gaccgccttg cggtcttccc tggtggcaat ctcggcccga ctgatattgg ctcgacattc
    22201 agcaatggcg gaagagacat tggcgaggac gcccgtcttg tccacagcca tcaccgagat
    22261 tttgacggcg tgcgtactgg gtgtggcact atcccattcc acttccacca atcgctcgcg
    22321 atcgtagtcc agcgcctcaa gattcggaca gtcgacggca tgaatagtca atccacgccc
    22381 gcgcgtgatg taaccaagaa tccggtcgcc gggaacggga ttgcagcacc gggacaactg
    22441 cattaatacg tcgcgcgcac ctttcacttt cacgcctttg tcgtcggact tgccggccac
    22501 ggccttgcga gtgctcggcg tcgcacgttt ttccgggacc agacttgccg atgaagcggc
    22561 cagcttctcc atcacgttgg ccgtcggcaa atggccgaac ccgaccgatg cgatcaattc
    22621 gtccgtcgta tcgtacccgg cctgccgggc agcggccgcg agttcagagg acttgagcat
    22681 ttgcgccggc gccatgccgt gccgacggaa ctccgattcc agcagccggc gcccgatctc
    22741 caagctgcgc ttctgttcct cgattttgat ccaatgtttg atcttggttt tggcccgaga
    22801 ggtgcggacg aatttgagcc aatccttgtg aggcgtttgc gtcggagacg tgagaatctc
    22861 gatcgtgtcg ccgctttcaa cctgatgctt cagcggcaca atctttccgt tgaccttggc
    22921 tcccacgcag tggtcgccga cctcggtgtg aatggcatag gcaaaatcaa ccggcgtcga
    22981 tcctcttgga agctccttca caatcccttt cggtgtaaag acataaacga catcgtggaa
    23041 gagatccagc ttgacggaat ccatgaactg gcggttgtcc ggcaagtcac gatgccattc
    23101 gacgaattga tgcagccagc cgaaggtctt gctgtcgcgg tctgtcacgc ggccctgttc
    23161 cttatatttc caatgcgcgg caatgccgca ctccgcaacc cgatgcatct cctccgtcct
    23221 aatctgaaat tcgacgtgtt cgcctttggg accgaccaca gtcgtatgga tcgattggta
    23281 gagattcgac tttggaatcg cgatgtagtc tttgaaccgc ccgggaaccg gacgccacag
    23341 agagtggatg acacccacga gcgcatagca attcattttg gtgtcagtca cgatacgcaa
    23401 ggcggtcaaa tcgtatactt cctcgaacga gatcgactgt ttgttcatct tctgatagat
    23461 gccgtagagg tgttttggcc tgccgtacac ctgtccgggc agtccgtgtt cctgcagggc
    23521 cttggtgacc agttcgatga cctcctgaat gtactgctgc cggtcttcat ctcgtttggc
    23581 gacgcgggtc cgcaagacct catagacgtc cggcttgaga tgcttgaggc agagatcttc
    23641 cagctcgttt ttgatccagc cgatgcccag gcgattggcc agcggcgcgt agatttcgag
    23701 cgtctcttcc gcaatggcat gccgctttgc ttggccgaga tgctcgagcg tgcgcatgtt
    23761 atgcagccga tcggccagct tgatgagcac gactcggata tcgtccgcca tggaaagcag
    23821 catcttgcgg aaattttccg cctgcttttc ttcatggctc ttgaaggtga tcttgccgat
    23881 ctttgtgacg ccgtcgacaa gatgcacgac gtcttttcca aactcgcttt ccagctcctc
    23941 gggggttgcg accgtatctt caagagtgtc gtgcagcaag gcggcgacga tggccgtcac
    24001 atcagtcctc aaaaacgtca gcacgcctgc gacggcgatc gggtgttgca ggtacggctc
    24061 gcccgagcga cgccgctggc cttcgtgcac tttggctgag aactcgtagg ccttccgcac
    24121 gatgttcaag tccgcttccg cgttgtaact cttcacgcgg tccagcagtt gatcaatgtc
    24181 tctgaccgtt tgattcacca tcaggcggtt ccccgatcgc tgacgcggag atcgcgaatg
    24241 cgcagctgaa tcctgtcata gccgttccag tgattgaact cgggtaggaa tgccagatcg
    24301 accggcgtgc cagcgggaat gccacgctcg atcaaggatt tcatcccgaa gccaatgctg
    24361 tcgaagggca tcgatcgtcc ttgccgaacc gtcatcttca aatgcttctc tcctaccgtc
    24421 cgcgcattca tcacttcaag acccttgacc gcgaaggtcg gctccggatt tccggcgccg
    24481 aacgggtgta acgtaccgat ctcgttgatg agcctggccg taatctcgga taattgtacc
    24541 tccgcatcga cttgcaatgt cggaacggtc gtttctcccc cattccactc cgccacgacg
    24601 gccgaaaacc gctcccgaag cgcctcgagg tttgattctc gcaccgtcac acctgcggcg
    24661 ctcggatgcc cgccgaaggc ctcgaggaga tcacggcagg cggcaagccc ttgatacaaa
    24721 tcgaatcctc ccgtggtcct cgcagatccc ttgccaatgc ctccctcatc tatcgcgatg
    24781 acaatcgccg gccgatgaaa cttctcgacc aaccgagccg ccgcgatgcc cacgaccccg
    24841 acgtgccacc gacgggacgc caggaccaca gccgccggta attcctggtc ttttaaagat
    24901 gcaacggctt ccgccatcag ttcggcctca atcttctgcc gctcgcgatt gagctgctcc
    24961 aaccgatcgg ctaactgctg cgcttcggct ggagactcgg tggtcagcag ccgaactccc
    25021 aacatcgcgt gatctaatct gccggcggca ttgagccttg gggccaactt gaatgcaatc
    25081 gtctcggctg tacagtcgcg taccgctccg gcgacctgct tcaacgccct gatgccgcaa
    25141 cgagcgccac gcgaaatttg cttaagaccc tcacgtacaa agccgcgatt ttcgtcatgc
    25201 aacggcacga cgtccgcgat ggtggcaagc gccaccagat cgagcagcga ttccaatggg
    25261 acgccggcct cgccataccg cagctgatag gcctgcgcga ctttgtaggc cagccccgct
    25321 gagcacaacc caagaaaggg atactgcgca tccgcacgat ggggattcat gacggccacg
    25381 gcgggaggca gttcctcatc cgtttggtga tgatcggtca cgatcacatc catgcccagc
    25441 tgggcggcca aggccacttc ccgatgcgat gtggtcccgc agtccgaggt gacgaggagc
    25501 gaaatcccct cgtcatgcag ccattggacc gccttcgcat tcaatccgta gccttcccgt
    25561 ttcctatggg ggatgtaggc ccgcacattg gcgccgagcg atccaaagaa agagaggtac
    25621 acagccgtcg ccgacatccc gtcgacgtca taatcaccat aaaaacaaac cggctcatgc
    25681 gccaccacgg cccgatgcag tcggtcaacc gcctgatcca tgtcgggaat caagaagggg
    25741 tcgtgcggga actggtgtga catccaggtc gtggcttgat cgggagtagt caccccgcgg
    25801 gccaacaata atgaggcggt cgctgatgaa attgagagag cctgcgccag ggcagcgcgg
    25861 ttggacggat tgatatcgcg gaagacccag agcttggact tcatcccacc tcgaagaagc
    25921 ctgagcctct cggcacacct aaactgctga gttttcagcg catagaagaa tcgggccgga
    25981 tactataagc tcaccggagc tttgtcaaac cagcagagga aaggagagga atagagagtg
    26041 cggtaggaag cacgagggag agcgagctac tctccccctt tttgcacgta gttcttgacg
    26101 aattcttcgg cgtttttttc gagcacgtcg tcgatttcgt caaccagctt gtcgatgtct
    26161 tctttaagtt tcttccccgt ctcgacgacc ttggggttgg ccttgacttc ttctttaccc
    26221 tggggttccc gtctgacctc ttgcttgcgt tcctgttttt ccatcgcata cctcccagat
    26281 ggaatcgtgg atccgccctt agcctaagat cacgatactc taggaggtca gcaccgtcaa
    26341 gcttcgacgc ggaacgggca tcattgccta gtgttttacc gagttgttcc tgaatgccac
    26401 ctctacttca cgatcagcga gacgatgagc agagccacaa tattgatcac cttgatcatc
    26461 gggttcactg ccggaccggc cgtatcctta taaggatcgc cgacggtgtc gccagtgacc
    26521 gccgccttat gcgtttcact gcccttttta ccctgttctt cgatgaattt cttcgcattg
    26581 tcccacgcgc cgccgccgct cgtcatagag atcgcgacga acaggccggt gacgatgctt
    26641 ccgaccagca cgccgccgag cgcttgtggc ccgagcacca cgccgacgat cacgggcgac
    26701 aggacaggga ttagtccggg aaccatcatt ttttgaatgg cggcttgtgt gacgatgtcc
    26761 acgcaggtgg cataatccgg cttccccgta ccttccatga tccccttaat ggtgcgaaat
    26821 tgccgccgga cttcttccac gatcagtcca ccggcttcac cgaccgcctt catgcacagg
    26881 gcgccgaaga taaacggcag catgccgccc aggaataacc ccacgaggac tttaggattg
    26941 gacaagtcga aggccgccaa tgcggcattg tgtgcagcca cttcacggga atactcggcg
    27001 aaaagcacca cggcggcaag acctgcagag ccaatggcat accccttcgt caccgcctta
    27061 gtagtattac cgaccgcatc gagcggatcc gtgatatccc tgacggcttt gcccagatgc
    27121 gacatttcag caatgccacc ggcattgtcg gtaatcggcc cgaacgcatc gatcgccacg
    27181 acgattccag ccatcgataa cattgatacg gccgccacag ccacgccata cagtcctccg
    27241 gattccaccc cgccacagat ccagaagctc ccgaggatcg ccgaagcgat gaccatgaca
    27301 ggggccgccg tcgcctgcat gccgaccgcg agtccggcaa tgatattggt cgcatgaccc
    27361 gtctcactcg ctttggcaat atactgaacc ggcgcatagc tttttgacgt gtaatagtcg
    27421 gtgatgaaca ccaacgccaa ggtcacgacg agtccgatca aggctgcaat gtagtagttc
    27481 atgccactca cgccgccgat gccttccatg acttgcgtcg tgaccgggta aaacgcgatc
    27541 gccgcgatcc cgcccgcgac gaacaggcct ttatacagcg ccggcatgat ttccccgcct
    27601 ggccccacct tcacgaagaa aatgccgatg atcgtcgcaa agatcgttac gccgcccaac
    27661 gccaatggat agaggattgg tgcgctggcg cctttgaaca tcgtgtatgc caacaccatc
    27721 gcggcgacgg tggtcacggc ataggtttcg aagaggtctg ccgccatgcc ggcgcaatcg
    27781 ccgacgttgt cgccgacgtt gtccgcaatc acggccgggt tgcgtgggtc gtcctcggga
    27841 attcccgcct cgaccttgcc gaccaggtcc gccccgacat cggccgcctt ggtgtagatg
    27901 ccgccgccga cacgcgcgaa caccgaaatc aggctgccgc cgaatcccag actcagcaag
    27961 gcgtgtacgg ctttctcctg gcccgagatc gattgcgcga tcatgaaaaa tcccgtgatg
    28021 gcgaggagcc ccagtccgat caacaacaac cccgtgacag cgccgccgcg aaaggccacc
    28081 gtcaaggcgg cattcatccc ctgatgcgcc gcctgcgccg tacgcacgtt tgcacgcact
    28141 gcgatgatca ttccgacgta gccggcaatc gctgaggctc cggctcccac aaggaaccct
    28201 accgccgtca gtatcccgaa cttgtccgag accgcgccgg ccgcggccag gaccacgaag
    28261 agcactgccg ctacaacgcc gacggtcttg tactgccgat tcatgtacgc accggcgcct
    28321 tcttgaatgg ccttggcaat ttcctgcatt ttggcgttcc cggcgtccaa tctgaatacc
    28381 cacatcgcca gatagagacc gtagccgatg ccagcacagg cggcaaccaa cgcaaacgtc
    28441 acaatggccg aatcactcac tggatgtcct cctcccaggt tacgaagtct gtcgtcacaa
    28501 ccattccctt tgcgctcacc atacgtcaca cttcatgtgg atcgaggggg aaccgaaggt
    28561 ggtgcagagc ggctgaggca actgcattga aaaaacgcgc cattctatac gcatggggcg
    28621 gggggatcaa ggtgtaaaag gcagtaaatg ctcaggcgag acatctaagg tgaaggccca
    28681 gcttgtcaaa ggaaacaaaa tcgctataat aggcccgcag atgacaggca aaaccctagt
    28741 tcacacctcg gtccgaccct tgcttcaccc ctcaatttgg atggttttcg gattggtttc
    28801 cctgctgctc gcggcgacgc ccgctgtagc ggagttgctc gaagtggctg acgtgatcgc
    28861 acggccccag cattacgatc gaaaagaagt ggtcgtcatg gggcaggtca gcgggatcca
    28921 ggcgatcacc gacaagcaag ggcagcccgc cttccagttt ttgttgaaag ataacattgg
    28981 caccataaaa gtgattagcc gtagagaagt tctagacggt gaccaaatta tcgtcgaagg
    29041 cacgttcact cgccgccgcc agggcggacg cctcgccgtc tacaatgaag tgaatgccat
    29101 cgtgatccgc cctctcaacc agttcgacgc agacctcgtt ggatagtccc gcaccttccc
    29161 aaatttccta ggcggtttac tagtacacac tcagggaatc ccgtaggaca gtgtgtggag
    29221 ggagatgtcg tgcgccatct cagggcgtac gtgtgacagt caataccaca aggaggattc
    29281 catgaagtcg attatgttgt catttctcgc ggccgggcta ctttgcttcg gcataggtgt
    29341 cgcgcaagcg gccaatccga tgtcagagaa agaagcctcg cccacgctca aagagcgcat
    29401 caccaaggat acggtgaagg gtaccttgat gaagacggaa ggcgagtatt actggattaa
    29461 ggacaccgac gggaaagaag tgaagattca cgtcgacaaa agcacgaaga tggacaaggt
    29521 cgtcgtggga gacatggtca aggcctacat cactgacaag ggccacacca ccacactgca
    29581 acgcgctgac tagctgtttg cgaccacgtt atgacgccgg caacctgccg gcgtctgctc
    29641 atccgcacat gattgttcgc cgcgaagggg ggtgtgtgca tggctcgcta cggtaaggct
    29701 gctcagaaat cggtgaagcg cacgatgcat aagcgaaaaa aagggacact gaccagcggc
    29761 aagagcaaga aacgggtaaa aaaccgcaaa caggcaatcg ccatcggatt gtcggaggcc
    29821 agacggaaag gcgccaaagt tccgcgaaag aaggcggcgt aacgctcagc gtccatcagc
    29881 tgcctggttt tgcacggccg tcctggcctt tccaggacgg ccgtttttgc tctcctcttg
    29941 tggccctccc cacaccagag aacgatggca ggagaaacac caatgggcgc accacatcgc
    30001 aatgcgaccg atacctttca tcactttgcc aggcagttgg cggaagcagt cggctcgccg
    30061 tgggcattcc tgctggcctt gatcgtcgtc atcgtatggc tcgcgagcgg ttcgaccttc
    30121 cactattcgg acacgtggca actggtcata aacaccggca ccacgattgt gacctttttg
    30181 atggtgttcc tcatccagaa cacacaaaac cgggatgcca aggcgattca tctgaaactc
    30241 gatgaactga tccgcggcgt ggcgggcgct cgcaataggt tgatcgatct ggaggagctg
    30301 tccgaagccg agcttgacga cttgcaacgt cagttcgcga agtttcggcg acagcgttag
    30361 tgagggtcct tcccagttca agaagcgagc cgaattgtag aagctactca caacatacag
    30421 gaaggagccc cacatgccca acaccaacgc ctcgttttct ccgcgcatgc gcagcgatga
    30481 gctcaaggaa cagatcggaa aactcgaacg gctggagcac cgtacgcctg tcctgccgac
    30541 cctggatagt ttcgatgaag agactgaagc gctcttgggc cgcctttacg gggacagtca
    30601 caaatacctc gaagcctata agtatgctac cgtcgcggag agcgaagcgc tggtcaacct
    30661 tccggaatct gcgcaagaac cgatggccaa ggatttaccc aagaccgccg tccaacagcg
    30721 ccgccaggtc ttgcttggca ttctcacgga aatgcaggat atggaagcca acgaagtcgc
    30781 gatgctgacc ggagaggacc gagaagaccc tccgagtatc tagccgccca cctggtacca
    30841 taccttgact atgtcgagtg catgattatg tgatccccca tgcgacatgg agatctcatg
    30901 gagatttgta ccatgctcgg ggaggtcaac atgaagaacc tgcgcaaaga ggttcgtgag
    30961 ctcattagca taaacgaaaa aatccagagc gctctgctgc atggagatcg catgaccgac
    31021 gatgaagcga ccctgatccg ccagtgtgcg agtgagctgc tggaaaagat cccgcctcca
    31081 tctcgaactt ggataagagc cggccgaacg gaggagggcg gtgaagcggt cgcagggaat
    31141 ccgtaagccg ctcagtcgag cgacaaaaaa ggcaacttct gtacaaacct cgcatgcgac
    31201 aggcttcaga caaggtacgg ctctgagact gcactggccc atccacctgc cctaacatca
    31261 gatttcctct taatctatga ggcaccgcgg caccgggagt cctattttcc gagtccgcga
    31321 ctgtcgacga tccaatggca ccccccttgc ttacctgtga aacggttcat ccgcacaagg
    31381 aggggcagca tgaagaacgc agctggattc atcctcattg gaacgcttgg ccttgggtcg
    31441 atgctgatgc aggcctgtgc tagccaatca ggttcagtcg ggggcgatga atttgcccag
    31501 gaacagatta ccgagcccat gataaaacac attcctcctc cggaatacag catgaacacc
    31561 tctcggcggc cgtcccaacg agcggagctg tcagcggtaa atgcgacggg tctgccgttc
    31621 ggcgccttgt ctgacgtgct cttcgatttt gatcaggccg tgttgcggcg cgatgccctt
    31681 cccttggtag aggcaaacgc gcgacgcctc gtgcaggacg gagtcacgcg cttattgctc
    31741 gagggtcgcg gcgacgaagt cggcacgtcg gcctacaaca tcgtgctggg cgagcgccgg
    31801 gcgagaagtg tgaagtctta cctgcaggag cgaggacttt cgcttcagct gaagacgaca
    31861 agctatggca aagatcgtcc gctgtgttat caacacaatg aagaatgcat gcagaagaat
    31921 cgcagtgtcc actttttagt gaaagactaa tccggcattt ctcaccgcaa taggtgaaga
    31981 caggtgtaag gcccgcttct taaaggaagc gggccttatc tttttgcgtt gggaaaacgt
    32041 tccggtcgct atccctaatc acgttcacgt gagcaagcct ccaccaaatg atcctggaac
    32101 ttcaataaca ccatcgttgc gtcaagagcg ctgatccttg tttaaaagaa tggcccgccc
    32161 cgctggccta ttaccccctt cgtccatcag gctgaattac atgggttttt ttaataccgc
    32221 cgtgggcagg agtctcaact aaacaaacga tgtctaggga agttcctagt gggagccact
    32281 ttgtag
//
LOCUS       DASSCQ010000146        48665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_831976, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000146 DASSCQ010000000
VERSION     DASSCQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 48665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48665
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831976"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..859)
                     /gene="fabG"
                     /locus_tag="VFH05_09985"
     CDS             complement(116..859)
                     /gene="fabG"
                     /locus_tag="VFH05_09985"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET6678589.1"
                     /translation="MSLQGRVAIVTGAAQGIGRAIAETLAEAGADVVVADLDPVRSQD
                     AVAAIEKIGRKALNVKVNVADSNDTKTMADQVLKQWGKIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGTFHCTKAVLQPMTKQRYGRIVNIASIVGAMGNVGQANYAASKAA
                     VIGFTKTAAREYASRNVTVNAVAPGFIDTAMTQGLSPEVKEALQKQIPLARLGTPADI
                     ANAVRFLVSEDAAYITGHVLHVNGGMLMS"
     gene            complement(877..1815)
                     /gene="fabD"
                     /locus_tag="VFH05_09990"
     CDS             complement(877..1815)
                     /gene="fabD"
                     /locus_tag="VFH05_09990"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET6678590.1"
                     /translation="MNPAVGFLFPGQGSQSVGMGKALFEAHSELKQVYAEATEVLGYD
                     IADLCFNGPAETLNLTEHTQPALLVSSVAALRVLESAAITPLAVAGHSLGEYTALVAA
                     GGLTYRDAVAIVQKRGRYMAEAVPAGTGLVAALLGLATDAVKEACREAASVGIVQAAN
                     FNSPGQVVIAGEKAAVERAIELAKSKGCKKAVPLPVSVPVHTPLMQKAADRLAKELAA
                     ITWSDLRMPLINNAEARPIVKAGDIPASLVRQLPSSVLWEDSVMRMASMGIKTFVEVG
                     PGTVLTGLVRRIVPDAVTLNVNDPKSLTATRTALGL"
     gene            complement(1940..2914)
                     /locus_tag="VFH05_09995"
     CDS             complement(1940..2914)
                     /locus_tag="VFH05_09995"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET6678591.1"
                     /translation="MRAHITGTGSYVPSKILSNADLENMVATSDQWITERTGIRARAV
                     CGPGEACSDLGVKAAQRALEDAKISASDVDMILLATCTGDYPLPATACLIQQQLGAKR
                     AAACDLSAACCGFVYALSIADAYIRTGMRHVLVIGSEVMSAITDWTDRNTCVLFGDGA
                     GAAVVSATEGDHGILSTHLRSDGALWELIAVPGGGSRLPPCEKVLAEKLNCIKMKGNE
                     TFKVAVRTLEEIARETLTANRLTVSDLDLYVPHQANVRILKAVADRLGLPLEKVMLNL
                     DRYGNTSAASIPIALDEAIRQGRITKGSLVMLGAFGAGLTWASALIRW"
     gene            complement(2911..3954)
                     /gene="plsX"
                     /locus_tag="VFH05_10000"
     CDS             complement(2911..3954)
                     /gene="plsX"
                     /locus_tag="VFH05_10000"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET6678592.1"
                     /translation="MTTSSRMKIALDAMGGDHGPVPCIEGALQAAQELNVDVILVGDE
                     KVLSDACHRLGCTDGRLSIKHAPQVVEMHESPAAVARKKRDSSIWIATELVKSGEAQA
                     VVSPGNTGASMVASFFVLGLAKGVERPAIATSLPTLTGEAIMLDVGANVDCTAQHLIQ
                     FAIMGNEYGQHLFKKPNPRIGLLSIGEEDSKGNEVTKEAFKLLRNSSLNFIGNVEGRD
                     VYSGNADIVVCDGFIGNIALKISEGVADTIKKLLFKEIAGSFFGRLAYPLIATPLVKL
                     KRRIDYAEFGGAPLLGVNGVTIICHGRSSAKAIKNAIRRAKGLAESHVDQLIQRDIEE
                     SRCQSRPAEEPPA"
     gene            complement(4157..4342)
                     /gene="rpmF"
                     /locus_tag="VFH05_10005"
     CDS             complement(4157..4342)
                     /gene="rpmF"
                     /locus_tag="VFH05_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET6678593.1"
                     /translation="MPNPKHKHSRARRDKRRTQKLRVTPPGMSVCPQCHELKLPHYTC
                     LNCGTYKGKAVIQVEEV"
     gene            complement(4370..4981)
                     /locus_tag="VFH05_10010"
     CDS             complement(4370..4981)
                     /locus_tag="VFH05_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET6678594.1"
                     /translation="MDIQAGMTMGVLTPLVADISTEGLSLVGEATAEELGLTAADATL
                     RGPVSVSLDLMQADDMIAVTGVLEGTAVRECVRCLKEYEDPLAFSVHAAFAREGKEFK
                     PGVRPLKGAEPRKGRPTPGKPEADAEPEDEGDDRYFYQGDHIELAPMLREHIILASPM
                     QPLCRDNCAGLCARCGKDLNEGPCQCPTEAPPSAIRVIRTTKN"
     gene            complement(4997..5269)
                     /gene="rpsR"
                     /locus_tag="VFH05_10015"
     CDS             complement(4997..5269)
                     /gene="rpsR"
                     /locus_tag="VFH05_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET6678595.1"
                     /translation="MERERGGSGGGRGDGGGGGRFFQRRRPCRFCIEKGSIDFKDVGL
                     LRNFLTERGRIVPRRISGNCLGHQRELTTAVKRARHIALISFAEER"
     gene            complement(5278..5691)
                     /locus_tag="VFH05_10020"
     CDS             complement(5278..5691)
                     /locus_tag="VFH05_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET6678596.1"
                     /translation="MAGFNKVILIGNLTRNPELRYTPNGTPVAALGLAVSRRYKQGDD
                     LKEEVCFVDIVVFGKQAEHCGQYLSKGNGIIVDGRLQQRRWETEDGQKRSKHEVVAQT
                     VTFLPKRGDGGGGQHAGGESGMHEDSNYEYEEPQG"
     gene            complement(5678..6025)
                     /gene="rpsF"
                     /locus_tag="VFH05_10025"
     CDS             complement(5678..6025)
                     /gene="rpsF"
                     /locus_tag="VFH05_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET6678597.1"
                     /translation="MELYESLFIIRPSVSDEETNALIEKMKGVADRTGAQFIKSENWG
                     RKKLAYEVRRERKGTYAYFYFKAPNNTVGELEHAYRLEDNIIKFLTVHLEKELVPRRV
                     AETPSEEFTGGRV"
     gene            6213..7391
                     /locus_tag="VFH05_10030"
     CDS             6213..7391
                     /locus_tag="VFH05_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678598.1"
                     /translation="MDRQIVYVDIPSFGIALARAKNAALRNRPVAMAPVHTPRALIRE
                     VSAEASSQGLQPGMSVELARLLCPDLRLVAPDVSHAQAAHRELQQRVLSFAPVWESIR
                     PGSLFLDLTGTTRLLGAPIDIATRIGRELTQHHRWHSVIGLAGNKLVSQLAATTLHRP
                     PQVLWIHPGSEQPFLAPLPSMWLPGLPRSRASRLVQRLEDLNLRTLGAIASVSVAQLE
                     AAFGASAALLHDWALGVDSSPVRSPATQPMIEWTLSLDPDEVDDHLLLGRLYGLLERM
                     CATLRHQQRICRFLRLSVRHSDHVERDAQERLTHGTYWEADLQPVLARLFYRCMRRRV
                     RLTRLTLQAGRLEPPAEQLSLFDVWTSAPPASHRLSRALDAIRAKFGEQSLSWGRTLR
                     "
     gene            7388..10465
                     /locus_tag="VFH05_10035"
     CDS             7388..10465
                     /locus_tag="VFH05_10035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6678599.1"
                     /translation="MMPPFLHLHVHSEYSPMRGVSSLAALCRSAGQDGFSAMALTDTN
                     GLYGAIRFIEEAKQQGLRPILGAELTADNHRAVLLAKTLAGYASLCRLLSERHCDPSF
                     DFIQAVARYRDGLIVATDDEVALRTWASESRQDLYVELTPGPAMHRTVWFSRRETLPC
                     VATNRAYFSRAHEFATHRLLRAIATNTTLSRLPAQECCAPTQWLMPGTLMASQFPHVP
                     DALENTIRIAEACHSDWRFGDTIFPAFRRLTDDDAFAELKAKTYQGALARYGRLSSDV
                     RKRIEKELAVIRQKSFAHYFLVVEEIVRQAPRTCGRGSVAASIVSYCLQITHVDPLKH
                     HLFFERFLNPGRKDPPDIDIDFPWDERDQILAWVFQHYGDRQAAMVANQNSLGFRAAI
                     RETAKVYGMSAAEITAIASRITRQTGLLQFADVPSNEQWVNRLSQALRLQAPWPEILA
                     QALRAQHHFRHLSMHCGGLVIVPDEIRRYVPVEFTAKGLPVIQWEKDQTEEAGLVKID
                     LLGNRSLAVIRDAIAAVACHTGRTIDYASWDPLTDEATRDLIRRGDTIGCFYIESPAT
                     RLLLKKLWKGMPADRLAVADVFDYLVMVSSLVRPAAITFVNDFVRRAHGHSHDPLHPL
                     MESILEETHGIMVYQEDVTKVAMALANFSLEDADQLRKVISKKHKHRQLRDYYHQFCR
                     GAAKNGASPATIAKIWTMIMSFAGYSFCKPHSASYAQVSFKSAYLRAHYPAEFMAAVI
                     SNEGGFYSTFAYLSEARRMGLAILPPDVNTSDWAYRGEGDRLRMGLMQVKTIPKAVAT
                     AIVSERDQAGPFRSFQDFLRRVPLESMHVRALIRAGCCDSICGELTRPALLWRVFAGK
                     EAGLLPVPDEYPASQKRGHEIEAFGFLASCHPLTLYRKQIEQLRPLPASQMPQFIGCR
                     VTMVGWLITEKPVETKHGLAMEFITLEDHTALYDATLFPDVYRRRCHVLSSNRAYVVQ
                     GLVEEAFGVATLTVLDLHPLDSTTDVAWSHEKTWYDGLCDAQPDQPTHSSPLT"
     gene            10417..11055
                     /locus_tag="VFH05_10040"
     CDS             10417..11055
                     /locus_tag="VFH05_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET6678600.1"
                     /translation="MRRPARSTDSFIPSHLSLPTLREAAARCTGCDLYKQATQTVFGE
                     GATQASILLVGEQPGDQEDRAGHPFVGPAGKILDKALSEAGIDRRDLYVTNAVKHFKW
                     EPQGKRRKHKKPSAAEIAACRPWLEAELQVVQPRVVVCLGATAAQSVFAKAVRLGDMR
                     GRAWSTPHAASVFVTVHPSAILRHPESAQREQEYRRFVQDLHHMTSYLRKAA"
     gene            11063..11635
                     /locus_tag="VFH05_10045"
     CDS             11063..11635
                     /locus_tag="VFH05_10045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET6678601.1"
                     /translation="MPHSRRSAKAGLLIIDMINEYRFDDADKLFPAIEQTATHIADLK
                     RQMKARSLPVVYINDNFGKWQSDFRKLVTRCLHEECRGKTVAQILLPDEDDYFVLKPK
                     HSGFYATPLELLLRYLQVERVIITGVAGDNCVFFTAADAYMREFEVTVPSDCVISIDA
                     DANRSALNHMRVTFKAEIGPSEALLTHLAA"
     gene            complement(11755..12846)
                     /gene="ychF"
                     /locus_tag="VFH05_10050"
     CDS             complement(11755..12846)
                     /gene="ychF"
                     /locus_tag="VFH05_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET6678602.1"
                     /translation="MGLCCGMIGLPNVGKTTVFNALTGGGALAANYPFATVDPNTGIA
                     LVPDPRLLTLTEIFKSKKTTYSTLEVRDIAGLVEGASKGEGLGNQFLAHIREVDALLH
                     IVRCFHGTDVVHVNGSVDPLRDIGVIETELMLSDLDTLDRRKQKTEKKVRAGDKKAAF
                     EVEFLTRLMGLLDKGEWLGNLIYTPEERLILSDCQLLAAKPVLFVANVSEDMNADEQT
                     VKKIRDFAQKRGARVVTICGQLEAELASLPESERAEFLNEMGLTESGLVRLTREAYTL
                     LNLITFFTAGETESRAWPIPRDTKAPQAAGKIHSDMERGFIRAEVYDYDDLLACGSEA
                     KVKEKGLFRLEGKEYVIKEADIVYFRFNV"
     gene            complement(12912..13517)
                     /gene="pth"
                     /locus_tag="VFH05_10055"
     CDS             complement(12912..13517)
                     /gene="pth"
                     /locus_tag="VFH05_10055"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET6678603.1"
                     /translation="MRLIVGLGNPGAAYAQTRHNAGMWVLERAAARWSIRLVRHGTAQ
                     RGSGRQGSALIELAGTLGFMNVTGPPLKGLLRELQLTSDDLILVHDDLDLEPGRLRIK
                     QAGGHGGHNGIKSVIESLGTSQFVRIKIGIGRPAPMQDSADYVLETVTKDEMDVIEPC
                     LERAVDALEILIHRGTETAMNQFNARERDASNGEENLKTEN"
     gene            complement(13520..14317)
                     /locus_tag="VFH05_10060"
     CDS             complement(13520..14317)
                     /locus_tag="VFH05_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET6678604.1"
                     /translation="MRFDLAVTVREKSGKGAARQLRRSGKVPGVLYGQGECLLLTIEP
                     EPLTKILKSQAGSTALITLTVNGAKAKPKRTALLRDFQVDPVEGYVLHADLFEISMDK
                     PIRVKVPVHVIGGTPAGVKEGGILHYNTRELHVECLPGALPDHIEVDASPLTIGQGIH
                     LKEIAKTEGVRYLDDPDQMVVSVAVPMSDAKLEALLTSQAAGPEGAKEPEVVAKGKAA
                     VEGAEGVAAAPGAAAPAGGEAKAGDKKEATAAAAAPKAEKKEAEKKK"
     gene            complement(14366..15307)
                     /locus_tag="VFH05_10065"
     CDS             complement(14366..15307)
                     /locus_tag="VFH05_10065"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET6678605.1"
                     /translation="MSRELMIFSGNANLALAHEICAYLGAKLGEATVSSFSDGEIRIK
                     IEENVRGADVYVVQSTCCPVNDSLMELLIMIDALKRSSAYRITAVMPYFGYARQDRKD
                     QPRVPITAKLVADLITTAGADRVLTMDLHAGQIQGFFNVPVDHLYALPVLLDYITKKK
                     VSDLVIVSPDAGGVERARAFAKRLQAHLAIIDKRREGPNQTQIMNIIGDIKGKSTLLL
                     DDMIDTAGTIVQGAQACADKGAREVWTACTHAVLSGPALERLQQSCLTQVIITNTIPI
                     QEKQRLCPKIHQLSVAPLLGEAIRRIHADESVSSLFA"
     gene            complement(15501..16421)
                     /gene="ispE"
                     /locus_tag="VFH05_10070"
     CDS             complement(15501..16421)
                     /gene="ispE"
                     /locus_tag="VFH05_10070"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET6678606.1"
                     /translation="MTNGESGSFHSGPSSITVHAPAKINLILRILDRRSDGYHNLWSV
                     MQTVALEDEVQIRVTSPGGIRLSCDTELLSVDSSNLVYRAASAVLEQASLSIGLDVEL
                     RKRIPMGAGLGGGSSDAAATILGLNQLLKLGWSREQMQSVAEQLGSDVPFFLFAPAAV
                     VSGRGEAVKPVWMDESRWVVLAKPGFGVETKWAYQELAATRADVRPLSSAHTAIDRHS
                     RLTWTQLATAAENDFEAPVFARYPVLRDIKEALRLQGAELALLSGSGATMFGIFSTES
                     AARRAQAHLARDQRLQVFVVPTCSEPVTVS"
     gene            complement(16414..17796)
                     /locus_tag="VFH05_10075"
     CDS             complement(16414..17796)
                     /locus_tag="VFH05_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6678607.1"
                     /translation="MEKILVVDDEKSLREVMSIMLKRAGYEVTEASDGEQAIGQVNKE
                     IYDLVITDLRMPRADGMDVLKAVKSSSPETVVLVVTAFGTADSAVEAMKQGAYDYLTK
                     PFQVDEVQLIIRNALEKRRLSTENMLLKREMASQSSFAQIVGQSEAMQKVFDVVRKVA
                     DSKSNVLICGESGTGKELVARAIHYNSARSPMPFVAVNCSAVPETLLESELFGHMKGS
                     FTGAVANKAGLFEIANGGTILLDEIGDTTPAIQVKLLRVIQEREFRRVGGNQDVKVDV
                     RVIAATNRELEKAVADGSFREDLYYRLDVIPIRLPPLRLRAGDIPLLIAHFLEKFSHE
                     SGKAAPTLTPEAMHVLLQHEWRGNVRELENLIERVVAFSTGTVSDTDVRGWLHHALAP
                     QQSQGVPTELPEDGLDLEGLINGIEKDLLMRALERSKWVKKKAARLLKLNTRSFRYRL
                     EKYAIKGGRD"
     gene            complement(17781..19478)
                     /locus_tag="VFH05_10080"
     CDS             complement(17781..19478)
                     /locus_tag="VFH05_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6678608.1"
                     /translation="MDVLRTRLQWLMGLRVTFVTLMLGLSLIFQATKGERVDTFYTLI
                     VLTYALTIPSAVLLRTLKTPSALTTFFWIQVGIDFLLETVLVARTGGVESPFAVLYVI
                     TVAVASLIPQRRVGLFAACTCIIVFGVVTNAQLYGFASSWGSVPAGHVGAADVFQTFG
                     VYALALLVVGLLSGTLADQLYRVDQSLREKEQGLNRLQAFHENIVRSISSGIFTADAT
                     GALTSFNPAAQELTGYTAGQVLGRAWREIFNWHPNQPSDDATAGALPAMTRFEVECKN
                     ANGNRLVLGMTVSPLYEQREEHGLVGVFKDLTQIRELEAEMRRKEWLANLGEMSAGMA
                     HEIRNPLGALAGAMQMLRKESTADATDRRLMDIAIREATRLDAIITEFLQYARPPELD
                     LAEHNINKILAETMDLVQHEARIRKRITIATSLAVGPLVVQVDQNQMRQVFWNLATNA
                     FDAMPDGGQLAISTGCRHVDAAGRSGDIVEIAFQDTGEGIAQDNLDKIFLPFFTTKKE
                     GSGLGLAAVHRIVDLHGGWIKVESQPQRGTRFVVCLPRSGGDGVRLWHEGRTPWKRS"
     gene            complement(19549..20763)
                     /locus_tag="VFH05_10085"
     CDS             complement(19549..20763)
                     /locus_tag="VFH05_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6678609.1"
                     /translation="MATFAYVGRSRSGAVKKGELTAKTKDEAVDQLRKQSVVVTSLEE
                     KTGKGGLKLSFGSGLTDKDLVVFTRQFGTMINAGLPLVQCLEILSTQSENKVLRETVG
                     EVRGQVEAGSTFSDALRRHPKVFDDLYVNMVHAGEVGGLLDTILTRLAKHIEKAMKLK
                     GQIKSAMIYPSAILGVAGIVITVLMIFVIPIFAKMFEELSNGKVGLPGPTQLVIDISN
                     AFQSFWYIGVGLVVGAVTLVKKYYATPKGRMAIDRFLLKTPIFGDLIRKASVAKFTRT
                     LGTLLSSGVPLLDGLSICAKTSGNKVIEEALLNARVSISGGKTIADPLTQSQVFPKMV
                     THMIAVGESTGALDAMLGKIADFYEDEVDQAVASLTSLLEPMMMVFLGVVIGFIVIAM
                     YLPIFKMASAIG"
     gene            complement(20910..21446)
                     /locus_tag="VFH05_10090"
     CDS             complement(20910..21446)
                     /locus_tag="VFH05_10090"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6678610.1"
                     /translation="MNIPNSLTILRILLIPVYVGLLIYERFNGAMIVLLVAGVTDALD
                     GTIARAANQRTRLGAFLDPLADKLLLTSGFVTLSAIHLIPSWVTILVVSRDAMLLLGT
                     AVAQFTESNIDIAPTFFGKGATFLQLAYVLLVIFLSSRHLDLSVLNPLLIAMVAFTVS
                     SGCHYLYRGYRSVGASPT"
     gene            21497..21892
                     /locus_tag="VFH05_10095"
     CDS             21497..21892
                     /locus_tag="VFH05_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678611.1"
                     /translation="MSNEVNGLQSAVDRPILQLRLHEEIPMNVLASLFKRDGLVEKHQ
                     LEGVDPSDRYFNRAVLVNRTASGYSAKMMYEALTVEGLSHPTIAAAITELVVKLQSLG
                     FSRIRTRANFKGTKYLAEKETWIEYQDPA"
     gene            complement(21894..23018)
                     /locus_tag="VFH05_10100"
     CDS             complement(21894..23018)
                     /locus_tag="VFH05_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6678612.1"
                     /translation="MKGSRVIQAAMDQAVRDGVFPGGVLAVRLAGELKCLVVSGCLSS
                     DPTDLVQASTVYDLASLTKPLATVTSILLLVQSGTLDLDAPVEHTLDDLHGTPVGVAT
                     LRDLLQHRSGLPGWRPLYEGLTANGIEWGRSESVRQTVLHLIGQEPLKYRRGERSEYS
                     DLGFILLGLAVERASGKPLDEYFDESVARPLGAQPLCFNPTETESCSSRPAVAVSSIA
                     PTEWDEWRRQLLKGQVHDENAAAMGGVAGHAGLFGTAEAVLAVSDVWLRSYEGQRSML
                     EQRLVREFTRRASSCSQSSWALGWDTPTAPSSSGSHLSPESFGHLGYTGTSLWVDPRR
                     KLEVLLLTNRVHPTRKNEKIRAFRPLIHDVVFQEIVSGTK"
     gene            complement(23181..23693)
                     /locus_tag="VFH05_10105"
     CDS             complement(23181..23693)
                     /locus_tag="VFH05_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET6678613.1"
                     /translation="MKITPLDIQQMVFKTGFRGYDKEEVNRFLEEIAETVEELNRENA
                     VQREKITFLEQQLIELKRTEVTLSNTLVSAQSLAEDVKRSAQRESELVIKEAELKAGE
                     MIRQARVELSNTQRDLSVLQKQRLLMVERLRATLRTFERMLEVEEQEAFQDGASSAEH
                     KLEGESSPAL"
     gene            complement(23715..24011)
                     /locus_tag="VFH05_10110"
     CDS             complement(23715..24011)
                     /locus_tag="VFH05_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET6678614.1"
                     /translation="MFVVENFFTALASIVYYLLELYMYVVIARAVISWVNPDPWNPIV
                     QFLEKVTEPALAPIRRMIGWRLGVDVSPIILILLLMFLQRFIVPSLHQMATAIG"
     gene            complement(24011..24826)
                     /gene="proC"
                     /locus_tag="VFH05_10115"
     CDS             complement(24011..24826)
                     /gene="proC"
                     /locus_tag="VFH05_10115"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET6678615.1"
                     /translation="MGSQMAKKIAFIGSGHMAEAMIGGLLAAGVCDSDALWASDPVAA
                     RRDRLKSLFSIRVGAENRQAVTWADIVILAVKPQSLPPVLSEIGADLNRVLVISIAAG
                     VTIQSIASQASPGTRIIRAMPNTPALVREGITALAWGEEVREEDSLVARTIFEAVGRV
                     IPVEERLMDAVTGLSGSGPAYVFLVLEALADGGVKMGLPRQTAELLAVQTVLGAARMV
                     LETGTHPATLKDRVASPGGTTIAGLHQLEQGKFRATLMGAVEAATKRSQELAR"
     gene            complement(24819..25529)
                     /locus_tag="VFH05_10120"
     CDS             complement(24819..25529)
                     /locus_tag="VFH05_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET6678616.1"
                     /translation="MDAEAGDSIENRVAFVFNQIRAATRKAGRVAGSVQLVGATKSVA
                     IGDIRRAVDAGLTLAGENRLQEALPKIEALKDTGVIWHFIGRLQRRKVRSVVGIFDLI
                     HSVDSLELAHEIDRRARAAGLRQKILLEVNIGEEHSKAGFLGDEVAGVLPQLGALDHV
                     AVCGLMAIPPLMREPERARRYFRALRDLAARLRQQKISRISMDELSMGMSHDYEVAIE
                     EGATLVRLGTALFGERRG"
     gene            complement(25586..26320)
                     /gene="pgeF"
                     /locus_tag="VFH05_10125"
     CDS             complement(25586..26320)
                     /gene="pgeF"
                     /locus_tag="VFH05_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET6678617.1"
                     /translation="MTASVITVPAFGLPHDGVRHFFGTRHHTGTLVYETGVPAQTRDV
                     SGQSWMLSVKQVHGTDALIVDRPLTGSDRFEGGWDALITDQPGVTVAVRTADCVPVLV
                     YDRRRKVAAAIHAGWRGALAGILPKTIQLMVARFSTDEADVRVSIGPSAGSCCYEVDE
                     AVLEPLRSGSTEWSSLLKDRRGNRAKLDLKALVKRQAAQIGIDREHITTVNLCTICHP
                     DLFFSYRREGRVNGTMLNGITLMPHW"
     gene            complement(26361..27539)
                     /gene="ftsZ"
                     /locus_tag="VFH05_10130"
     CDS             complement(26361..27539)
                     /gene="ftsZ"
                     /locus_tag="VFH05_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET6678618.1"
                     /translation="MFSFQEDCLSPVRIKVIGVGGAGCNAVNTMITSGLARVDFIAAN
                     TDLQALDRSRAAYKVQLGPERTRGLGAGAKPEIGKESALESKDQIRECLEGADMVFVT
                     AGMGGGTGTGAAPIVASIARELGILTVGVVTKPFSYEGHRRMTHADEGIRDLRRHVDT
                     LLVIPNQRLLGIVDKATPLLDAFKVADDVLRQAIQGIADVITTTGHVNVDFADVRTVM
                     SHTGRAVMGMGVSRGTNRAIEAAQKAICSPLLEEGSVEGARGVLLNISGGPNMSLHEI
                     EEAASIIQQTADPEANIIVGQVINPDMGDDLVVTVIATGFERDDQPTPVGVASQARMA
                     RTGQPSFNGVAVADRPHKDLDRPTYLRRMGDHRESMERLAVAADDEWDVPTFLRKQAD
                     "
     gene            complement(27584..28819)
                     /gene="ftsA"
                     /locus_tag="VFH05_10135"
     CDS             complement(27584..28819)
                     /gene="ftsA"
                     /locus_tag="VFH05_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET6678619.1"
                     /translation="MAKRDQILVGLDIGTTKICAIVSEVTEEGTLSIIGVGSSPSRGL
                     RKGVVVDIESTVESIKKAVEEAELMAAVQINSVYTGIAGSHISAENCKGVVALKKAEV
                     TRDDIHRAIESARTLAVIPHERRILHVLPREFMVDGQEGVREPLGLSGNRLEVNVHVI
                     TGAVTSAQNIIKSVNRAGLDVVDIILQPLASSEAVLSQEERDLGVAMVDLGGGTTDLA
                     IYLDGSIRHSAVLPIGGQNLTKDLAIGLLTSQTEAEKIKVQHGVTRTELVQGHETVEV
                     PSVGDRPARTFSRRDIAEILEPRVEEMFELVRREITRAGYEGMLGAGVVVTGGTSLLE
                     GMPDAAEHVLNLPARRGVPTGIGGLRDIVSNPMHATGVGLLLHARRHTEETATVGLRS
                     GRPIGKMVDRMKSWMFEFF"
     gene            complement(28824..29693)
                     /locus_tag="VFH05_10140"
     CDS             complement(28824..29693)
                     /locus_tag="VFH05_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET6678620.1"
                     /translation="MRLLRRKPPAKAAGHRSNEWKGPRAAQSMDHRQRARQIERLTRC
                     RRALRLSGWLGGAVAVCWMIVMLSQQLGPMLHRGLEIKQVSVEGTHQVTKQEVIDRLA
                     LKKGLAQYQVSLSYLAERVRTHPWIKEATIERLPLHELRVTVVERTPAAIVRLGSEQV
                     LIDKEGVILARLGTRDEATLPLLTGVDGTLLAQGDLGIKQRLQSAVELARWMAESLDG
                     RIELDLAHPVNLVASARGVRFHFGSDALKDQWERFMKVKAAFRMPALDGRKHEGHDVD
                     LRYDNRVIVRERG"
     gene            complement(29690..30670)
                     /locus_tag="VFH05_10145"
     CDS             complement(29690..30670)
                     /locus_tag="VFH05_10145"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET6678621.1"
                     /translation="MKTERRRLTQARIGVLMGGQSSEREVSLRTGAAVHGALCRLGYD
                     AVTVDVGPTLSRDLQEQKIAVAFLALHGPGGEDGSIQGFLETVGIPYTGSGVQASAVG
                     MHKVTTKTVLAAHAIPVPAGMVIKRGERISHTQALRSTKLRWPVVVKPASEGSTIGVT
                     IVKKPAQWNPALSLAHRYDREAMVEAYIPGHEVTVSLLGRENETPVVLPAVEIVAPGG
                     FYDFSAKYEKGKTQYLCPAPLPAAVTKEIGVLALRAFEVLGCAGAARVDFRITPRGRP
                     YVLELNTVPGMTETSLLPMAAVQAGIGYEELTERILESALARMKNKEHAA"
     gene            complement(30695..31642)
                     /gene="murB"
                     /locus_tag="VFH05_10150"
     CDS             complement(30695..31642)
                     /gene="murB"
                     /locus_tag="VFH05_10150"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET6678622.1"
                     /translation="MNADGQRTKRPRTRSRFDRKALEAVVADLRGRIRFNASLEAYTS
                     FKIGGPADVLVEPADVEDVRRLVSRASAARIPIFVLGGTNLLIRDGGIRGIVVSLSKL
                     RGIHEEPGAVLYAEGGVGMPTLIGHAIRRSLSGLEWAAGIPGTVGGCLVMNAGTRLGE
                     MKDAVKAVRLITMKGELRDLPASSIPFQYRRAGLPKGIVVGVWLQLRQGLRAEIERVV
                     KDYLHYRKDTQPLTMPSAGCVFKNPPGDSAGRLLEAAGLKGARVGDAEVSMKHGNFIV
                     NRGHASAADVIQLIGKVRQAIKRKAGVRLDLELKIVGEA"
     gene            complement(31647..33137)
                     /gene="murC"
                     /locus_tag="VFH05_10155"
     CDS             complement(31647..33137)
                     /gene="murC"
                     /locus_tag="VFH05_10155"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET6678623.1"
                     /translation="MSTILLEQQGSDGTGQRNGFTSSQAAGGLRRTSMFRKTQHIHLV
                     GIGGSGMSGIAEVLLTLGYKVTGSDQQASDTTRRLEELGGRIFIGHHESQIGEAQVVV
                     ISSAVSPQNPEVTAAKAKQIPVIPRAEMLAELMRLKFGVAIAGAHGKTTTTSMVANVL
                     AQGGLDPTMVIGGKVNALGSHARLGRGELLVAEADESDGSFLRLSPTIIAVTNLDREH
                     LDHYGSMERIYESFLEFINKVPFYGLAVLCADDQRLRGLFPNIVKRYYTYGLSESDGV
                     TPDFRATDIVAKQGGSEFRAFFRTRNLGPFRLAVPGIHNVSNALAAIAIGVELDVPVD
                     LIRKALAAFTGVERRFHIRGESNGVMVVDDYGHHPTEIKATLAAAKQGWPERRLVVLF
                     QPHRYTRTRDLLEDFSKAFQDADRLFLTEIYAASESPIPGVSGARLAEAIRAVGHPPV
                     TFVERKDQLTDQVLPQLKAGDLVITLGAGDIWKTGPALLEKLAAAS"
     gene            complement(33103..34227)
                     /gene="murG"
                     /locus_tag="VFH05_10160"
     CDS             complement(33103..34227)
                     /gene="murG"
                     /locus_tag="VFH05_10160"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET6678624.1"
                     /translation="MTIVIAAGGTGGHLYPAVALAREFLHRDPMTKVLFVGTSRGLES
                     KVLAHEGFELVLINAKPVMGKGLTDVLKGLCAMPLSLWQCGRILSQRQARLVIGVGGY
                     TSPMMVLAAALKRIPRVILEPNAYPGLANKAVAPFVQRVFLAFESAARFFDQRKGRVV
                     GTPVRKEFLTQCRPPGRERQRGGHHVLVFGGSQGAKALNSAAIEGLPDLLKQRPHMTV
                     THQTGEADHDRVSQAYRRAGVTATVLPFVYDMPAAINAADIVVARAGAMTVAELTTCG
                     KPAILIPLPTAIYDHQMKNAKVMEAAGAAVVLPQADLTGATLARSVAQILDDPQRMKA
                     MEDASVGLRRLDAAEAIVRECYALIGDQRHVNHSLGAAGI"
     gene            complement(34230..35339)
                     /gene="ftsW"
                     /locus_tag="VFH05_10165"
     CDS             complement(34230..35339)
                     /gene="ftsW"
                     /locus_tag="VFH05_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET6678625.1"
                     /translation="MDHVLLVVTLTLALMGLVMVFSASAIVAGNRFQDPEFFLKRQIA
                     WLGFGFLLMHLTSRIDYPLWKKLSMPILISMAILLVMVLVPSLGVAAKGARRWLRWGP
                     ISIQPAEMVKLVSVIYLATYLAKKADKLTLFRSGLLPALIVMAVLSGLVLLEPDLGTV
                     VVMGLVTVSLLFLGGARIGHLVSLGLCAIPVIFILVLSSSYRRQRLMTFLAPWKDASD
                     AGFQITQSFLAFGSGGPFGVGLGEGKQKLFFLPEAHTDFVLALVGEELGLAGTAAVIV
                     LFGLFVWRGFHIAARARMPFGKYLGMGITLLIGIQALVNAAVVTGLLPTKGLTLPFIS
                     YGGSSLVVSFVGVGILLSISRDRHGGASKGGRSGD"
     gene            complement(35449..36858)
                     /gene="murD"
                     /locus_tag="VFH05_10170"
     CDS             complement(35449..36858)
                     /gene="murD"
                     /locus_tag="VFH05_10170"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET6678626.1"
                     /translation="MLEVEEVDLSGAQVTVLGLARSGVAACHLLREAGARVTVADRKE
                     EVELATVLRTVDRNSVQVSVGSQYERSLERADLVVISPGVPYRMEALERVRRRGVQVI
                     SELELASRFLSAPILAVTGTNGKSTTVTLIGKMLAEQGKRAFVGGNLGTALSEAAWTT
                     LQAKKQGKPDPYEYLAVEVSSFQLETIDRFHPWAAAILNVTVDHQDRYDSIDEYVAAK
                     RRIFENQTSDDFALFNLDDPRVANLKSTVQARRLGFSRTQSIGAGFDGGTYLDGDEIV
                     TTVAGGRQHICRKSDLKIIGHHNVENVMAAATYAALWGCPFDIISRVVTAFPGLEHAL
                     ELVRERRGVRFVNDSKGTNVDATLKALEGIERPIWLIAGGRDKGGDFSRLTEAIRRRV
                     KHVVLIGEAAPLLKAAWTGATTISEAESLRDAVEWAAREAAAGDVVLMSPACASFDMF
                     QDYQDRGRQFKSAVQSLPE"
     gene            complement(36864..37940)
                     /gene="mraY"
                     /locus_tag="VFH05_10175"
     CDS             complement(36864..37940)
                     /gene="mraY"
                     /locus_tag="VFH05_10175"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET6678627.1"
                     /translation="MLYNLLYPLHTEYGFLNVFRYLSFRIIYAAVTAFLIAFVLTPWL
                     IRKLQEIKLGQQIRDDGPKHHVAKSGTPTMGGLLIIFAVLLSTMLWADITKPYVWLVM
                     IATLGFGAIGFVDDYLKFIKARSKGLSPTQKFSAQIAVALLIAVALYFLPSYSTRLNV
                     PFFKNFMPDLGWFYIIFVVLVIVGSSNAVNLTDGLDGLAIGPIMIASLAYTIVAYVAG
                     HRIMSDYLLIPHIDGAGEIAVFTAAILGSSLGFLWFNTYPASVFMGDVGSLPLGAALG
                     TVAVISKHELLLLMVGGVFVIEAVSVIFQVASFKSRGKRIFLMAPIHHHFEMKGWEEP
                     KVVVRLWIIAILLALLSLSTLKLR"
     gene            complement(37971..39422)
                     /gene="murF"
                     /locus_tag="VFH05_10180"
     CDS             complement(37971..39422)
                     /gene="murF"
                     /locus_tag="VFH05_10180"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET6678628.1"
                     /translation="MGLLTVEELREVISVKVLAGDPSLSMRRRIRNISTDSRSIRRGD
                     LFVALKGERFDGHDFVQAVLAKGATGAIVHDTYRWEAKTQRSSGISRHDPFLFGVRDP
                     LFAYQQLATHHRCRFGIPVVAVTGSNGKTTTKDMVASVLGQRWRTLKTEGNFNNRIGV
                     PHTLLRLTARHQAAAIEMGVDHRGQTTRLCEIVRPTIGLITNIGPDHLEFFGGMEGSA
                     QAKAELLDMLPADGTAVLNADDAYFDYLAARAQCRVISFGLSERADVRASDLSTDVRT
                     GTTFGLILPGRSRPMSVTIKVPGTHNVTNALAAAAVGFSLNLPGALIAQGLARFRPAA
                     MRSQVMTHQGVHIINDCYNANPASMKAALKVLADWAPARARVAVLGDMLELGRQALPL
                     HREVGQFAAALKLTRLIVCGDLGREIAVGAREAGMSSHAIDQLPDASSAADLLKKTIR
                     QGDVVLVKASRGMRMEQIVQGLTGMKSVTKQAS"
     gene            complement(39464..40984)
                     /locus_tag="VFH05_10185"
     CDS             complement(39464..40984)
                     /locus_tag="VFH05_10185"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET6678629.1"
                     /translation="MQPRALTVTQLLAALNGQVAIVDRLGDLDGSITSVTDDSRSVSS
                     GSLFVAVKGERVDGHKYVGQAIKAGAAAIVGQDFVETASVPFVQVTDSRKALGLIGSR
                     FYGDPSAQLAMIGVTGTNGKTTTTYLCKALLENIGRRVGLIGTVAYQIGAETIPASHT
                     TPGAVELQRLLARMNEAQLDSVVMEVSSHALAMDRTAGCEYDVAVFTNLTQDHLDYHR
                     TMEEYFQAKLRLFTGLGQGKKTRQRAIVNMDDPRGVYVRAACRVPVWGYALNVSADLV
                     AENVRLSMNGSTFTAATPAGTFTVESRLVGEHNVYNLLGAIGVALHRGATCEQVREAV
                     ARVTNVPGRFERVSAGQDFTVVVDYAHTEDALVRLLTAARTLKTQRIITLFGCGGDRD
                     RGKRPKMGRAAVEFSDVVILTSDNPRTEDPLAILREVEAGVCEALQRRPSVQYHMVAD
                     RREAIRTAVGLARPGDILLIAGKGHEDYQIVGTKKMHFDDREVAREAIQQLRKNCA"
     gene            complement(40993..42702)
                     /locus_tag="VFH05_10190"
     CDS             complement(40993..42702)
                     /locus_tag="VFH05_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6678630.1"
                     /translation="MGPSRGRRYSLLVLMLAGFGIILFRLVTLQVLQAAELTARADRQ
                     HQKSVTLEGARGTVVDRHGKVLAMNVEVPSIFGVPTSLDNPANAARFLSPVLHIRREE
                     IEKKLRQEKHFVWLARKIEPEQGHRLEQLSIDGIGMVMEGRRFYPKGPLLAHVLGFVG
                     MDGIGLEGLERRYETQLHGEKRMTILQRDALGRTVFPKGLREQVPAAGHALTLTIDEV
                     IQYIAEKELEQAVDHSRAKSGTIIVLEPQSGAILAMAVSPRFDPNAVAALAADRWRNR
                     ALTDTYEPGSTMKVVVAAAALEERVMMPGSMLFGENGRMTVASTTIHDHEKLGWMTFA
                     EMIQKSSNIGAAKTGMLLGESRLYRYLQAFGFGQRTDVDLPGEVAGLLKSPREWGRRS
                     LASISMGQEVGVTPLQMVSAVAAIANDGVLMKPYVVSGMRDQKGQLVKETFPHVKRRV
                     VSPVTARTLTTILEGVVTSGTGTKAAIPGFRVAGKTGTAQKVDPRTGAYSSALSVGSF
                     VGFVPADSPRLAMIVVIDEPQGEAWGGVVAAPVFRRVGEQVLTYLGVSRDDQVKIAMV
                     APQ"
     gene            complement(42704..43027)
                     /locus_tag="VFH05_10195"
     CDS             complement(42704..43027)
                     /locus_tag="VFH05_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET6678631.1"
                     /translation="MKSVVITAGLCLVFFFVWERVDIVRLGYHIERLKAQKVLLERER
                     DQLQVKFSALTAPERIAKVATDKLGMVPPQQGQVLIVHPQQAPPPSLEQVRIARTIST
                     AKGER"
     gene            complement(43024..43980)
                     /gene="rsmH"
                     /locus_tag="VFH05_10200"
     CDS             complement(43024..43980)
                     /gene="rsmH"
                     /locus_tag="VFH05_10200"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET6678632.1"
                     /translation="MAWENVGEIGGSQFGGNREHIPVMPAEVLFWLRPRSQGIYLDCT
                     VGYCGHALDILEASDPDGKVFGIDRDPEAVDAARRRLQPFGDRAVIIKGHFMELKALL
                     VEHNISHVEGVLFDLGASSPQLDDPARGFSFQSEGPLDMRMDRSGPTAADIVNGLDEI
                     GLADMIYRWGEERYSRRIARALVRARQQRTISTTRELVSVIESAVPGHYRRGRIHCAT
                     RTFQALRIAVNNELDHLEASFRDAADVLAPGGRLCVISFHSLEDRIVKQSFKAFSIRN
                     PGEFEVLTKRPQVPTEDEMRRNPRSRSAKLRVLQRIAHEGHA"
     gene            complement(44212..45504)
                     /gene="asnS"
                     /locus_tag="VFH05_10205"
     CDS             complement(44212..45504)
                     /gene="asnS"
                     /locus_tag="VFH05_10205"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET6678633.1"
                     /translation="MPVMTIDAAAQHVGQEVTLRGWLRSRRDSGKLHFLTLRDGTGDM
                     QAVVSKKAVGDDQFALSASLTQESSLIVTGQLRQDHRAPGGYELDVRSLEALQIAGPF
                     PIQPKEHGVGFLMEHRHLWLRSHRQHAILRIRHEIIRACRNFFDERGFVLVDAPIFTP
                     NACEGTTTLFQTQYFDETAYLTQSGQLYSEATAAAFGKVYCFGPTFRAEKSKTRRHLM
                     EFWMVEPEVAFAQLADMMDLAEEFLAAIVQQVLKTRRTELTTLERDISKLERVVPPFP
                     RLTYEEAVGILRKKNNPTQMGDDFGGDEETILSQEFDRPVIIHRYPAALKAFYMQPDD
                     HRPDLALCMDVLAPEGYGEIIGGGQRIHTLELLRTRLQEHRLPEEAFKWYLDLREFGS
                     VPHAGFGMGIERAVAWICGLEHVRETIPFPRMLYKLYP"
     gene            45631..46650
                     /locus_tag="VFH05_10210"
     CDS             45631..46650
                     /locus_tag="VFH05_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678634.1"
                     /translation="MYPGRHLGSHFAMAIVAVSATYGALSLSSALAASAIYYCADRRA
                     DQLISATPGPGCVPLADTKLEPRDESIQGKGKQDFHVENLEQDVSAFLKRYRHFLECC
                     KNDLSELSHVEELGDEVNELLISVQGNLSNNALASRAIMLRELIPRVAKARSDLKTLR
                     ATLEKISELSATRDQLDFEEAGRQSQRIRELEESIEREIHAPKLPGSAKTGANIGVAP
                     AAGPAIGRSSKTGTDIGGIGSSGQDIGASPRSSREIGQSGPSGFEIGATGRAGPAIGQ
                     SSLNQETSSDVGSTLQRSTVGSTISDSTVGSSINSSTIGSDLRDASGSSSPENSASRP
                     GENTR"
     gene            complement(46654..47703)
                     /locus_tag="VFH05_10215"
     CDS             complement(46654..47703)
                     /locus_tag="VFH05_10215"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678635.1"
                     /translation="MISGATTRRNRFMKRLTVAWLFLPILLVACNRNEPIVVIQLHPK
                     NPDIIYVTTNDYIYKSRDGGQTWANISKGMSHSRVISMAVDPSYPATVYAGTKGDAVY
                     KSHDGGQRWTSMRSGLDDATISSVVNQFVFDPFDSNHIFVATTMGIFETKSGGGSWAK
                     RMEGMKEVLMVVTLGMDPTRPSILYAGTSGGVYKTVNEGTHWEKVNNGLVPPEMVKTS
                     RALNVTSIEVDRFAPDTVYAATLSGVFKTTDGARSWSRIGELLSDQMIMSMILDRTKK
                     GVVYLAGRDGVHRSDDGGMTWKTLNNGLTTTNVRSLVQSPIDPDLLYAGTNGSGLYRS
                     RDAGESWTPMPPLTP"
     gene            complement(47700..48197)
                     /locus_tag="VFH05_10220"
     CDS             complement(47700..48197)
                     /locus_tag="VFH05_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET6678636.1"
                     /translation="MADQFSFDVVSEVNMQELKNALDQATKEIKQRFDFKDSKTEITL
                     KEKEKELVVLSDDEYKLKAVQDIIKGKCVKRGVSLKAFDYGHIEPALSGTVRQTAKIQ
                     SGLASEKAKEIAKTIKDSKLKAQAQIQGEQVRILSKSKDELQSAIALLRGRDFGIDLQ
                     FTNYR"
     gene            complement(48221..>48665)
                     /locus_tag="VFH05_10225"
     CDS             complement(48221..>48665)
                     /locus_tag="VFH05_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HET6678637.1"
                     /translation="TIWPMFGAANQLLGTLALCIGTTVLIKMRKSSYLWITALPMLFI
                     GVVTLTGSYEMFWMFLRKAAASAAEDAVALYLDAVLVAVVALLGLIVLSDSMRQWYGY
                     VVLKQPFTSTEIVVMTGGSSAGSLPAMVHDEHDGGFRLPKGTGCC"
BASE COUNT        10204 a        14925 c        13125 g        10411 t
ORIGIN      
        1 ccataaacgt gcaatcctcc ttccagatct gtcggttcct ctactgtcgt atccgccgcg
       61 ggacattgtc cgcagttatg tggtctgtca tccaagctca agcggcgaag gtctattaag
      121 acatgagcat gccgccgttc acatgcagca catgccccgt aatataggcc gcatcctcag
      181 agacgagaaa gcgcacggcg ttcgcaatgt ccgccggcgt gccgaggcgg gcaagaggaa
      241 tttgcttttg cagcgcctcc ttgacctcag gagacaagcc ctgcgtcatc gccgtgtcga
      301 tgaagcctgg agccaccgcg ttgaccgtga cgttccggct ggcgtattcg cgcgccgccg
      361 tctttgtaaa gccgatgacg gccgccttgg atgccgcgta atttgcctgt cccacgttcc
      421 ccatcgcgcc gacaatcgaa gcaatattca ctattcgacc atagcgctgt ttggtcatgg
      481 gctgcaagac cgctttggtg cagtgaaagg tgccgtttaa attgacctga agcaccagat
      541 tccaatcttc ttccttcatc cgcagtagca gcccgtcgcg ggtgatgccg gcattattga
      601 ccaggatatc gatcttcccc cactgtttga gcacctggtc ggccatggtc ttggtgtcat
      661 tggagtctgc cacgttcact ttgacattga gggctttcct gccgatcttc tcgattgccg
      721 caaccgcatc ctgagagcgc accggatcga gatcggcaac gacgacgtcc gctcccgcct
      781 ctgccagcgt ctccgctatg gcccggccta tgccctgtgc cgctcctgtg acgattgcga
      841 cgcgaccctg tagtgacatg cctgctgctc cttgttctat agacccaatg ccgtgcgggt
      901 ggccgtcagg gattttgggt cattcacatt cagggtaacg gcgtcaggta cgattcgtcg
      961 caccagcccc gtcaggacgg tgcccggccc cacttcgacg aaggtcttga tgcccatact
     1021 cgccatgcgc atcaccgagt cttcccagag cacggatgag ggcagttggc gcactaagga
     1081 tgccggaata tcgccggctt tcacaattgg cctggcttcg gcgttattga tcaatggcat
     1141 gcgcaagtcg gaccaggtaa tcgctgcaag ctctttcgcc aaccggtcgg cagccttctg
     1201 catgagcggt gtgtgaacgg gcacgctcac gggcaatggc actgccttct tgcatccctt
     1261 gcttttggca agctcaattg cgcgttccac cgcggccttt tctcccgcaa tgaccacctg
     1321 gcccggagag ttgaagttcg ccgcttgaac gattcccacg gacgctgctt cccggcacgc
     1381 ctccttgacc gcatcggtgg caagcccaag aagagcggcc actaaaccgg tgccagcggg
     1441 caccgcttcc gccatgtatc gtccccgctt ttgcacaatg gcgacggcgt cgcgataggt
     1501 cagacccccc gctgccacga gcgccgtgta ttcaccgagg ctatggccgg cgacagccag
     1561 cggtgtaatc gctgcagact ctaaaacccg cagcgcggcc acactgctga cgagcaacgc
     1621 cggttgagta tgctccgtca ggtttaaggt ttccgcaggc ccgttaaagc agaggtcggc
     1681 aatatcgtat cccagcacct ccgtggcttc ggcatagacc tgcttgagtt ctgaatgcgc
     1741 ctcgaaaagc gcctttccca ttcctaccga ctgggagcct tgtccaggaa ataggaagcc
     1801 gactgctgga ttcataagcg gttagatatc ttcgaaatgc cctggaagtc aacgggaaaa
     1861 gttccccgga ggcttccgcc acggaccgca tcgtgctgct tgatgatgcg ggcaaccacg
     1921 tcgattgcgg aatgacagat taccaccgga tcagtgctga cgcccatgtc aacccggccc
     1981 caaacgcgcc taacatgacg agcgagccct tggtgatccg gccctgccgt atggcttcat
     2041 caagggcgat gggtattgac gcggcggatg tgttgccgta gcgatccagg ttcagcatga
     2101 ccttctcgag cggtaatccc aaccggtctg ccaccgcctt gaggatccga acgttggcct
     2161 gatgcgggac atacagatcc agatcggaaa cggtcagacg gttggcggtc aacgtctctc
     2221 gcgctatttc ttccaacgta cgcacggcga ccttgaaggt ctcattgcct ttcattttaa
     2281 tgcagttgag tttctcggca agcaccttct cgcaaggcgg caatcgtgag ccgccgcccg
     2341 gcacggcgat caattcccac agtgccccgt cggatcgtaa atgtgtggag aggatgccgt
     2401 ggtcgccttc agtggcgctg accaccgccg cccctgcgcc gtccccgaaa aggacgcagg
     2461 tgttccggtc cgtccaatcg gtgattgccg acatgacttc cgatccgatc accaagacat
     2521 ggcgcatgcc ggtccggatg taagcgtccg cgatcgacag agcatacaca aatccgcaac
     2581 acgccgcgga gagatcgcag gctgcggccc gtttggcgcc caattgctgt tgaatcaagc
     2641 aggcggtggc cggaagcgga taatcgccgg tacaggtagc cagcagaatc atgtcgacgt
     2701 cggaggcgct gatcttcgcg tcctcgagcg cgcgctgcgc tgccttgacc cccagatccg
     2761 aacacgcttc tccgggaccg cataccgcac gggcccggat gcccgtacgc tccgtgatcc
     2821 attggtccga ggtcgccacc atattctcca agtcggcatt ggagaggatt tttgaaggca
     2881 cgtaggaccc ggtccctgtg atgtgcgccc tcatgccgga ggctcctccg ccggccggct
     2941 ctggcaacgg ctttcctcga tgtctcgttg aatcaattgg tcgacatggc tttcggcaag
     3001 gccctttgca cggcggatgg catttttaat cgctttggct gacgaacgcc catgacagat
     3061 gatcgtcacc ccattcaccc cgagtagcgg cgcgccgccg aactcggcgt aatcgatccg
     3121 gcgtttcagt ttcactaaag gagtggcgat caacggatag gctaaccgtc cgaaaaacga
     3181 ccctgctatt tccttgaaga gcaatttctt gatggtatcg gcgactcctt cggaaatctt
     3241 cagggcgatg ttcccgatga agccatcgca gacgacaatg tccgcattgc cgctgtacac
     3301 gtcacgacct tcgacgttgc ctatgaaatt caatgagctg ttccgtaaca gcttgaacgc
     3361 ttctttcgtc acttcgttgc ctttactgtc ctcttcgccg atgctcaaca gcccgatccg
     3421 tggattgggc tttttaaaca gatgctgtcc atattcgttc cccatgatcg cgaattgaat
     3481 gagatgctgc gccgtgcaat cgacgttggc gccgacatcc aacatgatgg cttctccagt
     3541 caacgtaggc agacttgtcg cgatggccgg ccgttcaacg cctttggcca agccgagaac
     3601 aaaaaatgat gccaccatgc tggcgccggt attccccgga ctcaccaccg cctgtgcttc
     3661 tccgctcttc accaactcag tggcgatcca aatggacgaa tcccgtttct tgcgcgccac
     3721 cgctgcaggg gattcatgca tctctacaac ctgcggtgca tgcttgatgg acagacggcc
     3781 atcggtgcac ccaagccggt gacaggcatc tgaaagcacc ttttcgtcac cgacgagaat
     3841 gacgtcgacg ttcagttcct gagcggcttg aagtgccccc tcgatacagg ggacaggacc
     3901 atggtctcca cccatggcat caagcgcgat cttcatgcgc gaggatgtgg tcatgacgtc
     3961 cgcttaccct taagaaaaca ctactgaaga gttcgagtac ggggggagtg tagagcagta
     4021 ggcccaacga cgcaaccgat tggcgacgcg tagtcctgcg ccaacacacc ggtgcctgtc
     4081 acccgcgcca gccctgcgaa cagcgccttt ccatagacag tggcaacaca acggcggcag
     4141 ctttgacgtc cagcccttac acttcttcga cctggatgac ggctttcccc ttgtaggtcc
     4201 cgcaattcaa acacgtgtaa tgaggcagct tgagttcgtg gcactgtggg cacacggaca
     4261 taccgggagg cgtgacccgt agtttctgtg tccggcgctt atcgcgtctg gctctcgaat
     4321 gcttatgttt cggattaggc atcgtctttc ctccagcaac cagtctcgat cagttttttg
     4381 tggttcgaat tacccggatc gccgacggtg gcgcttcagt gggacactgg caaggcccct
     4441 cgttcaaatc tttcccgcat cgcgcacaca gcccggcgca attgtcccgg cagagcggct
     4501 gcatcggaga agctaaaata atgtgctcgc gtaacatggg cgccagttca atgtgatcgc
     4561 cctgatagaa atagcgatca tccccttcgt cttctggctc tgcatccgct tccggtttgc
     4621 ccggcgtcgg ccggcccttg cggggctcgg cgcccttcag aggacgcacg ccgggtttaa
     4681 attccttgcc ctcacgagcg aatgccgcat ggaccgaaaa cgccaagggg tcctcatatt
     4741 ccttcaggca tcgaacacat tctcggacgg ctgtcccttc cagcacaccg gtcaccgcaa
     4801 tcatgtcatc agcctgcatg aggtccagac tcacggacac cggacctcga agggttgcat
     4861 cggccgccgt tagcccgagt tcttcagccg tcgcttcgcc gaccagtgac agcccttctg
     4921 tcgaaatatc ggctaccagc ggtgtgagca ctcccatcgt catacctgct tggatgtcca
     4981 ttcctcagac cgtcttttac cgctcttcgg caaaactgat cagtgcgata tgccgggccc
     5041 gcttgacggc ggtcgtcaat tcgcgctgat gtcccagaca gttgccggag attcggcggg
     5101 gaacgatgcg cccccgctcc gtcaaaaaat tcctcaggag gccgacatcc ttaaaatcta
     5161 ttgagccctt ttcgatacaa aaccggcatg gccggcgccg ttggaaaaac cgcccgcccc
     5221 cgcccccatc gcctcgccct ccgccactgc cgccgcgttc acgttccatg ttgtccctca
     5281 tccttggggt tcttcgtact catagttgga atcttcatgc atcccggact cgcctcccgc
     5341 atgctggcca ccgccgccgt cgccccgttt cgggagaaat gtgaccgtct gagcgaccac
     5401 ttcatgcttg ctgcgcttct gaccgtcttc cgtttcccaa cgccgttgct gcaatcgccc
     5461 atccacgatg atgccgttcc ctttgctaag gtactgtcca cagtgttcgg cctgcttccc
     5521 gaacacaacg atgtccacaa aacagacttc ttctttcagg tcgtcaccct gcttatagcg
     5581 gcggctcact gccaagccca gtgcggcgac cggtgtgccg ttcggcgtat accgcaactc
     5641 ggggttgcgg gtcagattcc cgataagaat gactttatta aacccggcca ccggtaaact
     5701 cctcagatgg tgtttccgcg acccggcggg gcaccagttc cttttcgaga tggacggtga
     5761 ggaatttgat gatgttgtct tctaatcgat aggcatgctc caattcgccg accgtattgt
     5821 tcggcgcctt gaaatagaag taggcgtacg tacccttccg ctcacgccgg acttcataag
     5881 ccagcttctt tctgccccag ttctctgact tgatgaattg cgcgccggtc ctgtcggcga
     5941 cacccttcat cttttcgata agtgcattgg tctcctcatc ggagacggac ggacgaataa
     6001 tgaatagaga ctcgtagagc tccatgcaga gttcctcctg gacaaaaggc cccggaacgc
     6061 gtccggagcg aggtgtaggt gcgcggcctt tcagccggcg cgaagcgcag aaactaacat
     6121 agccgacaag ccggtgcaaa tagggtcttt catcgggttg acttttcttt catccgcttg
     6181 taaccattgg ttacattcca tggtttttgg atatggatcg gcagatcgta tatgtagaca
     6241 ttccttcgtt tgggattgcg ctcgcccgtg ccaaaaacgc tgcattgcgg aacagaccgg
     6301 tggccatggc gccagtccat acaccgcgtg ccttgattcg agaagtttct gctgaagcct
     6361 cttctcaagg actccagccg ggcatgtccg tcgaattggc gcgcctgctt tgccccgatc
     6421 tacggttggt cgctccagat gtatcgcacg cgcaggccgc gcaccgcgag ctgcaacagc
     6481 gtgtcctgtc ctttgcaccg gtatgggaat cgatccggcc tggctcgctg tttctcgact
     6541 tgaccggcac gacccggctg ttgggtgcgc ctatcgatat cgccacgcgc atcggtcgtg
     6601 aattaaccca acaccatcga tggcacagcg tgatcggatt ggctgggaac aagttggtct
     6661 cacagctggc tgcgacgaca ctgcaccgac cgcctcaagt cttatggatt catcccgggt
     6721 ccgaacagcc gtttctggcg ccgttgccgt ccatgtggct tcccggcctc cctcgaagcc
     6781 gcgcatcaag gctcgtgcaa cgactggaag atttgaacct tcgcacactg ggcgcgatcg
     6841 cttcggtctc ggtggcgcag ctcgaagccg cattcggtgc ctccgccgct ctgctgcatg
     6901 actgggcctt gggggttgat tcatcaccgg tccgctctcc tgccacacag ccgatgatcg
     6961 aatggaccct ctctctcgat cccgatgaag tggacgatca cctcctcctt ggacggctgt
     7021 acggtttgct ggaacggatg tgcgccactc ttcgccacca acagcggatc tgccgctttc
     7081 tccgtctctc tgtccgccac agcgatcacg ttgagcgaga cgcgcaggaa cgattgacgc
     7141 atggcacgta ttgggaggct gatcttcagc cggtgctcgc gcgcctgttt taccggtgta
     7201 tgcgccggcg agtgcgcctg acacgactga cgctgcaggc gggaagactg gagccgccgg
     7261 ccgagcaact gtccctcttt gacgtgtgga cctcggctcc gccggcgtcc catcggctgt
     7321 ctagggcact cgatgcgatt cgcgcgaaat tcggcgagca gtccctgtcc tggggaagaa
     7381 ccctgcgatg atgccccctt ttctccacct gcatgttcat tctgagtatt cgccgatgcg
     7441 cggggtgtcg tcgctggcgg cgctctgccg ctcggcgggc caggacggat tttccgccat
     7501 ggccctgacg gataccaacg gattgtacgg cgccatccgg tttatcgagg aagcgaagca
     7561 gcagggcctt cggcccattc tcggggcaga gttgacggcc gacaaccatc gggcggtcct
     7621 gctggccaaa acgctggccg gttatgcgtc gctctgccgt ctcctgtccg aacgacattg
     7681 cgatccctcg tttgatttta ttcaagccgt ggcgcgttat cgtgatggcc tgattgtggc
     7741 cactgatgac gaagtcgccc tccgaacctg ggcatcggaa tcgcgacagg acctgtacgt
     7801 cgaattgacc ccggggccgg ccatgcatcg caccgtgtgg tttagccgtc gtgaaaccct
     7861 tccctgcgtt gccaccaatc gggcgtactt ttcacgcgcg catgagtttg cgacgcatcg
     7921 tctcttgcgg gccatcgcca cgaatacgac gctctcacgg ctgccggcac aagagtgttg
     7981 cgcaccaacc caatggctga tgcccggcac tctcatggcc tctcagtttc ctcacgttcc
     8041 tgatgcactg gagaatacga ttcgtattgc ggaagcctgc catagcgatt ggcgtttcgg
     8101 cgacacgatt tttccggcct ttcgccggct gaccgatgac gatgcctttg ccgagctcaa
     8161 agcgaaaacg tatcaggggg cactggcacg atatggccgc ctatcgagcg acgtccgcaa
     8221 acgaatcgaa aaggaattgg ccgtcatccg gcaaaaaagt ttcgcccatt attttcttgt
     8281 ggtggaagaa atcgtccgtc aagccccgcg cacttgcggc cggggatcgg ttgcggcttc
     8341 gattgtctcg tactgtctcc agatcacaca tgtcgacccg ctcaagcacc atttgttctt
     8401 tgagcggttt ctcaatcccg gccggaaaga tccaccggac atcgacattg attttccgtg
     8461 ggacgagcgg gatcaaatct tggcatgggt cttccaacat tatggcgacc gtcaggctgc
     8521 catggtggcg aatcagaaca gtctgggctt ccgcgcggcc atccgcgaga cggcgaaggt
     8581 ctacgggatg tcagccgcgg aaatcacggc gatcgcttca cgcatcacac ggcaaacagg
     8641 tcttctgcaa tttgccgatg tcccaagcaa tgagcaatgg gtgaaccgac tctcgcaggc
     8701 gctgcgactc caagcgccat ggccagagat actcgcccaa gcccttcgtg cacagcatca
     8761 ctttcgccac ctctcgatgc attgcggcgg cctggtgatt gttccggacg agattagacg
     8821 atacgtgccg gtcgagttca cagcaaaagg cctaccggtg attcaatggg aaaaggatca
     8881 gacggaggaa gcggggctcg tcaaaattga tctgctgggt aaccgctcgc ttgcggtcat
     8941 ccgtgatgcg attgccgccg ttgcctgtca caccgggcgg acgatcgact atgcgagttg
     9001 ggatccactg acggacgaag ccacccgcga tttgatccgt cgcggagata ccatcggctg
     9061 tttctatatt gaatctcccg caacgagact cttgctcaaa aagctctgga aggggatgcc
     9121 tgccgatcgt cttgccgtgg ccgacgtctt cgactatctc gtgatggtct cttcgctggt
     9181 ccggcctgcc gcgatcacct tcgtgaacga cttcgtcagg cgcgcgcacg ggcactcgca
     9241 tgatcctctt caccccttga tggaatccat cctggaagaa acccatggca tcatggtcta
     9301 ccaggaagac gtcacgaaag tggcgatggc actcgcgaat ttttcattgg aagacgcgga
     9361 ccaactgcgg aaagtgatca gcaaaaaaca caaacaccgg cagctacggg actattatca
     9421 tcagttttgc cgcggtgcag caaagaacgg cgcctccccg gccacgattg ccaaaatctg
     9481 gacaatgatc atgagctttg ccggctacag tttctgcaag cctcattcgg ccagctatgc
     9541 tcaggtctcg ttcaagtccg cctatctgcg ggctcattac ccggccgaat tcatggccgc
     9601 tgtcatcagc aatgagggcg gtttttattc gaccttcgct tatctctcgg aagcgagacg
     9661 gatgggactg gcgatcctcc ctcccgatgt caatacgagc gattgggctt atcggggcga
     9721 aggagatcga cttcgaatgg gcttgatgca ggtgaaaacg attcccaaag ccgtcgccac
     9781 ggcaatcgtg tccgaacgtg accaggcagg tccgttccga tcgtttcagg attttctccg
     9841 gcgtgtgccc cttgaatcca tgcatgtccg ggcgctgatc cgcgccggct gctgcgattc
     9901 catttgcggc gagttgacac gaccggctct gctgtggcgg gtgttcgctg ggaaagaagc
     9961 cggcctctta cctgtcccgg acgagtatcc cgcttcccaa aagcgaggtc atgaaatcga
    10021 ggcgttcggc tttctcgcca gctgccatcc cttgacgctg taccgtaagc agatcgagca
    10081 actgcgcccg ttgccggcct cgcagatgcc tcagttcatc ggttgccgcg tcaccatggt
    10141 gggatggctc atcaccgaaa agccggttga gaccaaacat ggtctggcga tggaattcat
    10201 cacgcttgaa gatcatacgg cactgtatga cgctaccctt ttccccgatg tctatcggcg
    10261 gcgttgccat gtgctttcat ccaaccgggc ttatgtcgtc cagggacttg tagaggaagc
    10321 ctttggtgtc gcgaccctca ccgtacttga tttgcacccc ctggattcca ccaccgacgt
    10381 cgcatggtcg catgaaaaaa cgtggtatga tggcctatgc gacgcccagc ccgatcaacc
    10441 gactcattca tcccctctca cctaagtctt cccacgctac gagaggctgc tgcccgctgc
    10501 acaggatgcg acttgtacaa gcaggccacc cagaccgtct ttggagaagg cgcgacacaa
    10561 gcttcaatcc tcctggtggg cgagcaaccg ggagatcaag aagaccgcgc gggtcatccc
    10621 tttgtaggac cggctgggaa aattttagat aaggcgctat ccgaagccgg cattgaccgt
    10681 cgggacctgt atgtcacgaa tgccgtcaaa cacttcaaat gggaaccgca gggcaagcgc
    10741 cgcaaacaca agaagccatc ggccgctgag atcgccgcct gccgaccctg gctggaagcc
    10801 gaattacagg tcgtacagcc gcgggtggtc gtctgcctcg gtgcgaccgc agctcaatca
    10861 gtcttcgcca aggccgtacg actgggtgac atgcgaggtc gggcgtggtc gaccccgcac
    10921 gccgcctccg tttttgttac ggtccaccct tctgcgatcc ttcggcatcc cgaatcggcg
    10981 cagcgcgaac aagaataccg ccggttcgtc caggacttgc accacatgac gtcgtacctg
    11041 cggaaggccg cttaaacaga tcatgccgca ctcacgtcgc tctgccaagg ctggcctact
    11101 cattatcgat atgatcaatg agtatcgttt cgatgacgct gataagctgt ttcccgccat
    11161 cgaacaaact gcaacacata tcgcggacct gaaacggcag atgaaagccc gcagcctacc
    11221 ggtcgtttac attaacgata atttcggcaa gtggcagtcg gactttcgta aactggtgac
    11281 tcgatgtctg catgaggaat gccgtggtaa gaccgtcgcc cagattctgc tgccagatga
    11341 agacgattac tttgtcttga aaccgaagca ctcgggcttc tatgcgacgc cgctcgagtt
    11401 gctgcttcgc tatctgcaag tcgagcgagt gattattacc ggcgtggctg gcgacaattg
    11461 cgtcttcttc actgcagccg acgcctacat gcgcgagttt gaagtgaccg tgccttcgga
    11521 ctgtgtcatc tcaattgatg ccgacgccaa tcgctcggcc ctgaaccaca tgcgagtcac
    11581 cttcaaagca gagatcggcc cttcagaagc attattgaca cacctcgcgg cgtaacgaca
    11641 tgtgctgggt agggcgtttc tgagacagcg tcgcctgcgc acggatgtcc ggcatgtgga
    11701 acgtgtggat gtaacggcaa gaaccgagga aaactgagaa gacgtggcgg tcaatcacac
    11761 attgaaccgg aaatagacaa tgtcagcttc tttgatgaca tattccttgc cttccagtcg
    11821 aaacaaccct ttttctttca ccttggcttc tgatccgcat gccagcagat catcgtagtc
    11881 gtagacctcg gcacggatga agccacgctc catgtcagaa tgaatcttgc cggcggcctg
    11941 gggagccttg gtatccctcg gaatcggcca ggcgcgtgat tcggtctcgc cagccgtgaa
    12001 gaaggtaatg agattgagca gggtgtaggc ttctcgtgtc aggcgcacga gaccggactc
    12061 ggtcaatccc atctcattca agaactctgc tcgttcactc tccggcaagg aggcaagctc
    12121 agcctccaac tgaccgcaga tcgtcacaac gcgggcgccg cgtttctggg cgaagtcgcg
    12181 aatcttcttg acggtctgtt catcggcatt catgtcttct gacacattgg ccacaaagag
    12241 caccggcttg gcggcgagca gctgacaatc gctgaggatg agccgctcct ccggtgtata
    12301 gattagattg cccagccact cgcctttatc gagcagtccc atgagtctgg tcagaaactc
    12361 cacttcgaag gcggctttct tgtcaccggc tctgactttt ttctcagtct tctgcttccg
    12421 gcggtcgagt gtgtctaagt cggacagcat gagttcggtc tcaatcacgc cgatatccct
    12481 gagcggatcg acgctgccat tgacgtgcac gacatccgtg ccatgaaaac agcgtacgat
    12541 gtgcaacaat gcatccacct cacgaatatg cgcgaggaac tgattaccca gtccttcccc
    12601 tttactcgcg ccttcaacca aaccggcgat atcacgaact tcgagcgtac tataggtcgt
    12661 cttcttggat ttaaatatct ccgtcagcgt gagcagacgt ggatccggaa ccagcgcgat
    12721 cccggtgttg ggatcaacgg tggcaaacgg ataattggca gccagagctc cgcctccggt
    12781 gagggcattg aacaccgtag tcttgccgac gttcggcaag ccgatcatcc cgcaacaaag
    12841 tcccatacct gacgtcctga gtccggaaca gtgagccgtg gctgcaccca cctgcgtggt
    12901 gggcgcagga gttagttctc ggttttcaga ttctcttctc cattcgacgc atcacgttcc
    12961 cgagcgttga actgattcat ggcagtttca gtgccgcgat ggatgaggat ctccagcgcg
    13021 tccaccgctc gctcgagaca aggctcgatg acgtccattt catccttcgt gaccgtttcc
    13081 aagacgtagt ctgccgagtc ttgcatcggc gcggggcgcc cgataccaat tttaatcctg
    13141 acgaattgag aagtgcccag cgattcaatc accgatttga tgccgttgtg accgccatgc
    13201 cctccggcct gtttgatgcg aaggcggcca ggttccagat ccagatcgtc gtggacgaga
    13261 atgagatcgt cgctggtgag ctgcaactcc cggagaagac ctttcagcgg agggcccgta
    13321 acgttcatga acccgagggt gccggcgagc tcaatcaggg cagatccctg gcgaccggaa
    13381 ccacgttgag cggtaccatg ccggacgaga cggatggacc agcgagcagc ggcccgctcg
    13441 aggacccaca tgccggcatt gtggcgggtc tgggcatacg ccgcgccggg attgcctagg
    13501 ccgacgatga ggcgcaacgt tacttctttt tctcggcttc tttcttctcg gcttttggag
    13561 ctgcggccgc ggcggtagcc tctttcttgt cgccggcttt tgcctcacca ccagccggcg
    13621 ccgctgcgcc aggggcagct gccacacctt cggcaccttc gaccgccgcc ttaccctttg
    13681 cgaccacttc cggttctttt gcgccttcag gaccagcagc ctggctcgtc agcagggctt
    13741 caagcttggc atccgacata ggaaccgcca cgctcaccac catttgatcc ggatcgtcga
    13801 gatagcgcac cccttcggtc ttcgcgatct ccttcaagtg gatcccctga ccaatagtca
    13861 gtggcgaggc atcgacctcg atatggtccg gcaaggcacc cggcaaacat tccacgtgca
    13921 gctcgcgcgt attatagtgg agaatcccgc cttctttgac accagccggc gtcccaccaa
    13981 tcacgtgcac ggggaccttt actcgaatcg gcttgtccat cgagatttcg aacaaatcgg
    14041 catgcaaaac atatccttcg accgggtcca cctgaaaatc gcgtaagagg gcggtccgtt
    14101 ttggcttggc cttcgctccg ttgaccgtca gcgtgatcaa tgccgtgctt ccggcctggg
    14161 acttcaaaat cttcgtcaag ggttctggtt cgatcgtaag caacaggcac tccccttgcc
    14221 catagagcac gccgggcact ttcccgctgc gccggagctg gcgcgccgca cctttcccgg
    14281 atttttccct gactgtgacc gccaaatcaa atctcatggt actcctccgt ccccctctgg
    14341 cttttgagat gttcccgttt aagcgttagg caaacagcga actgaccgac tcatctgcgt
    14401 gtatgcgccg gatggcttca ccaagcaagg gcgccaccga caactgatga atcttcgggc
    14461 acagtcgctg tttctcttgg attggaatcg tattcgtaat gatcacttgt gtgaggcagg
    14521 attgctggag ccgttccagc gcgggtccgg ataggacggc atgcgtgcac gctgtccaga
    14581 cttcgcgcgc ccccttgtcc gcacaagcct gtgcaccttg aacaatcgta cccgccgtgt
    14641 cgatcatgtc gtccaataac agcgtgctct tccctttgat gtcgccgata atgttcatga
    14701 tctgtgtctg gttcggaccc tcacgacgtt tatcgatgat agccagatgg gcctgtaagc
    14761 gcttggcaaa cgcgcgggca cgttccacgc cgccagcgtc gggtgagaca atgaccaagt
    14821 cactgacttt ctttttcgtg atgtaatcca gcagcaccgg caaggcatac aggtggtcca
    14881 cgggcacgtt gaagaaacct tgaatctgtc cggcatgaag atccatggtg agaacccggt
    14941 cggcgcctgc cgtcgtgatg agatctgcca ccagcttggc ggtaatcggc acacggggct
    15001 gatcctttcg gtcctgcctg gcatagccga aatatggcat cacggccgta atgcgatagg
    15061 cagaggagcg tttgagcgca tcgatcatga tcagcaattc catcagggaa tcgttgacgg
    15121 ggcagcacgt cgattgcacg acatagacgt ccgccccccg cacattttct tcaatcttga
    15181 tgcggatttc cccatcgctg aacgatgaca ccgtcgcctc tccaagcttg gcgccgagat
    15241 aggcgcaaat ctcatgggcg agtgccaagt ttgcgttgcc agagaaaatc atcaattctc
    15301 tgctcatcga tccctcgaac acttttcctt atcgaaaatg gaaatactat cgtacgtggt
    15361 cgtttcgacg gcgggcatcg gtgggcgtat tctcgagccc accaccctca tgcgctcgcg
    15421 cggttgagga agccaattaa gggactgaac tgtaacggaa ttcgggcaag attgtcaacc
    15481 gagccggatc aggggcagca ctaggagact gtcactggct cagagcaagt tggaacgaca
    15541 aagacttgga gccgctggtc ccgggcgagg tgcgcctgcg cgcgccgggc tgccgattcg
    15601 gtgctgaaga tcccaaacat tgtcgcccca ctgcctgata gcagggcaag ctcagcaccc
    15661 tggaggcgca gagcctcctt gatgtcgcgt aacactggat agcgggcaaa gacgggggcc
    15721 tcgaagtcgt tttccgcggc ggttgctagc tgtgtccagg tgagccggct gtgtcggtcg
    15781 atcgccgtgt gggcagaaga cagcgggcgt acatctgctc gagtcgcagc gagttcctga
    15841 taggcccatt ttgtttccac gccgaatccc ggtttcgcca acacaaccca gcggctctcg
    15901 tccatccaca ccggtttcac ggcctcacca cgcccagaaa ccacggcggc aggtgcgaat
    15961 aaaaagaacg gcacatcgct gcccaactgt tcggcgactg actgcatctg ttctctcgac
    16021 cacccaagct tcaacagctg gttgaggcca aggatggtgg cggccgcgtc gctgctgcct
    16081 ccgccgagcc ccgctcccat tggaatccgt ttgcggagtt ccacgtcgag accaatggag
    16141 agagaggctt gttcgaggac ggcagatgct gccctatata cgagattgct ggagtctacc
    16201 gacaaaagct cggtgtcaca gctcaggcgg attccccctg gggaagttac acggatttgc
    16261 acctcgtctt ccagcgcgac cgtctgcata accgaccaga gattatgata gccatcggag
    16321 cggcgatcga ggatgcgaag aatcagattg atcttggcgg gggcatgaac cgtaattgaa
    16381 gagggaccgg agtgaaacga tcctgactcg ccgttagtca cgacctcctt ttatagcata
    16441 tttttccaat cggtaccgga acgatctggt gttcaacttc aagagacggg ccgctttttt
    16501 cttgacccat tttgaacgtt ccaacgctct catcaaaaga tccttctcaa tcccgttgat
    16561 aagcccttcc aaatccagcc catcctcggg gagctccgtc ggtacccctt gtgattgctg
    16621 aggcgcgagc gcgtgatgga gccatccacg gacatctgtg tcgcttacgg tgcccgttga
    16681 aaaggcgacg acacgctcga tcaagttttc cagctcccgt acattgcctc gccattcatg
    16741 ctgtaaaagg acatgcatcg cctccggcgt cagcgtaggc gcggcctttc cgctttcgtg
    16801 cgagaacttc tcaagaaaat gtgcgatgag cagcggaatg tcgccggccc tgaggcgcag
    16861 cggcggaagg cggatgggaa tcacgtcgag acgataatag agatcttctc ggaaggagcc
    16921 atcggccaca gctttttcaa gctcgcggtt cgtcgccgca atgacccgca catcgacctt
    16981 cacgtcttgg ttgccgccca ctcggcggaa ttcccgctct tgaatgactc ggaggagctt
    17041 gacctgtatg gccggagtgg tgtccccaat ctcgtccaac aaaatggtcc cgccgtttgc
    17101 gatttcgaac aaaccggctt tgttcgccac cgcgccggta aacgatccct tcatatgtcc
    17161 gaacagctcg ctctcgagta aggtttctgg cactgcactg caattgaccg cgacaaaggg
    17221 cataggactg cgagcgctgt tgtagtggat tgcccgggcg accaattcct tccccgttcc
    17281 gctttccccg cagatcagca cattgctctt cgagtcagcc accttgcgca cgacgtcaaa
    17341 aactttttgc atcgcctcac tttgtcccac gatctgagcg aaagaggact gactggccat
    17401 ttcccgcttg agcagcatgt tctctgtgga cagccggcgc ttttcaagtg cgttacgaat
    17461 gatgagctgg acttcgtcta cctggaaagg cttggtaaga tagtcatagg ccccttgctt
    17521 catcgcttcc acggcggaat ccgccgtccc gaaagcagtc acgaccaaca cgaccgtttc
    17581 aggtgaggag gacttcaccg ctttcagaac gtccattccg tccgctcttg gcatgcggag
    17641 atcggtaatg acgagatcgt agatctcctt gttgacctgg ccgatcgctt gctcgccatc
    17701 ggaagcttcc gtgacctcat atccggcgcg cttgagcata atgctcatga cttcccggag
    17761 actcttttca tcatcaacga ctaagatctt ttccacggcg ttcgtccctc atgccataac
    17821 cgcacaccat ctccacccga gcgaggcaga cacaccacaa agcgtgttcc ccgttgcggc
    17881 tggctctcaa ccttaatcca gccaccgtgc agatcgacaa tgcgatgaac ggcggccagc
    17941 cccaatcctg aaccttcttt cttcgtcgta aaaaacggga gaaagatctt gtctaaattg
    18001 tcctgcgcaa tgccctcgcc cgtgtcttgg aaagcgattt cgacgatgtc gccgcttcgt
    18061 ccggcggcgt cgacatggcg gcaccctgtc gaaatggcaa gttgcccgcc atccggcatg
    18121 gcgtcaaacg cgttcgtggc gaggttccaa aacacttggc gcatttgatt ctggtccacc
    18181 tggaccacca ggggtccgac ggccaatgac gtggcgatgg taatccgttt tctgatccgc
    18241 gcttcatgtt gtactagatc catcgtctcg gcaagaattt tgtttatatt gtgttcggcc
    18301 aaatcgagct ccggcggcct ggcgtactga agaaactctg taataatggc gtccagcctc
    18361 gtggcctccc ggatggcgat gtccatgagc cgccggtcgg tcgcatcggc cgtcgattcc
    18421 tttcgcagca tctgcatcgc gccggccaag gcgcccaacg gatttctgat ctcatgggcc
    18481 atcccggccg acatttcacc cagattggcc agccattcct tgcgccgcat ttctgcttcg
    18541 agctctcgga tttgcgtcaa gtccttgaac acaccgacca acccgtgttc ttcccgctgt
    18601 tcatagagcg gcgagacggt catgccgagc acgaggcggt tgccgtttgc attcttacac
    18661 tccacctcaa accgcgtcat ggcaggcagt gcccctgcgg ttgcgtcgtc ggagggttga
    18721 ttcggatgcc agttgaaaat ttctcgccaa gcccgcccga ggacctgacc ggctgtgtag
    18781 ccggtcagtt cttgcgccgc gggattgaat gacgtgagcg cgcctgtggc gtcggcggtg
    18841 aagatcccgc tactgatgct ccggacgatg ttttcatgaa aggcctggag ccggttcaac
    18901 ccttgttctt tttcgcggag cgattgatcg acgcgataga gttggtccgc cagcgtgcca
    18961 ctcagcaagc ctacgacgag aagggccaac gcatacacac cgaacgtctg gaacacgtcc
    19021 gccgcgccga cgtgcccggc cggcacgcta ccccaacttg acgcgaagcc gtagagctgg
    19081 gcattcgtca ccacgccgaa cacaatgatg caggtgcatg ccgcaaacag gccaacgcgg
    19141 cgctgtggga tgaggctagc gacggcaacc gtgatcacat agaggaccgc gaagggactc
    19201 tcgactcctc cagtgcgcgc gaccagcaca gtttcgagga gaaaatcgat gccgacttga
    19261 atccagaaga acgtggtgag ggcggaaggc gtcttgagcg tacgaagcaa gactgcagag
    19321 ggaatggtca gcgcataggt gaggacgatg agtgtataaa aggtgtcaac acgttcgcct
    19381 ttggtcgcct ggaagatgag cgacaagccc agcatgaggg tgacgaaggt cacccgaagc
    19441 cccatcaacc actgaagcct ggttcgcaga acatccatgc gttgttacgg ttcgaagagg
    19501 cccctcctgg accgggcatg cgaggaaccg gcggagcggc acaagagcct acccaatcgc
    19561 ggaggccatc ttgaagatgg gaagatacat cgcgatgaca atgaagccga tcacgacgcc
    19621 caggaacacc atcatcatgg gttcgagtaa tgacgtcagt gaggccaccg cctgatcgac
    19681 ctcatcttcg tagaaatcgg cgatttttcc gagcatggca tcgagtgcgc ccgtggattc
    19741 tcccacggca atcatgtgcg tgaccatctt ggggaacacc tgactctggg tcaacggatc
    19801 cgcaatcgtt ttgcctccgc tgatgctgac gcgcgcgttc aacagcgctt cctcgatgac
    19861 cttgttgccg gaggttttgg cacatatgga taatccgtcg agaagaggga ccccgctcga
    19921 aagcaacgtt cccagcgtac gggtgaactt cgcaacggac gctttgcgaa tcagatcgcc
    19981 aaatattggc gtcttcagga gaaacctgtc gattgccatc ctaccttttg gcgtcgcgta
    20041 gtatttcttc acgagcgtca ccgctccgac gaccaggccg acgccaatgt accaaaacga
    20101 ctgaaaggca ttgctgatgt cgatgacgag ctgcgttggc cccggcaagc cgactttgcc
    20161 gttggagagc tcttcgaaca tcttcgcgaa aatgggaatc acgaagatca tcaggacggt
    20221 gatcacgatg cccgcgacgc cgagaattgc cgacggatag atcatggccg acttgatctg
    20281 ccccttgagc ttcatggcct tttcaatatg cttggcaagg cgagtcaaaa tcgtatcgag
    20341 caagccgccg acctcgcctg catgcaccat gttcacgtac aggtcgtcga atactttggg
    20401 atgccgccgc aacgcgtcgg aaaacgtgga gccggcttcc acttggcccc tcacttcacc
    20461 caccgtttcg cgcagcactt tattttcaga ttgcgtcgaa agaatttcga gacattgcac
    20521 cagcggaaga ccggcattga tcatggttcc gaactgccgc gtgaaaacca ccaggtcttt
    20581 gtcggtcaga ccggacccga agctgagctt aagccctccc ttccccgtct tctcttccag
    20641 actcgtgacg actacacttt gcttgcggag ctgatcgacc gcctcatctt tggtcttggc
    20701 agtcagttca cccttcttga ccgctccgga ccgtgaacgc ccgacatacg caaaggtcgc
    20761 cataagtcac tctcaccttt tttgagaggt cctcaacaac cgaattttcg atggaaatac
    20821 ctacgtggcg agtctactgg tgacctgaaa aactgtcaaa caaatgcaca atctgtgcga
    20881 agtagcggcg cagcgagccc ggctaacgat caagttggag atgcaccaac agatcggtac
    20941 ccacgataaa gataatgaca gccggaggag accgtaaatg cgaccatggc gatcaagagc
    21001 gggttcagca ccgacagatc caagtgacgc gagctcaaga aaatcacaag cagcacgtag
    21061 gcgagttgca ggaaggtggc cccttttccg aaaaaggtcg gggcgatatc aatattcgac
    21121 tctgtgaact gcgccacggc ggttcccagc agaagcatcg catcgcgact gaccacaaga
    21181 atggtgaccc aggaaggaat gagatggatc gcggaaagcg tcacgaaccc tgacgtcagc
    21241 agcagtttat cggccaatgg atccaaaaac gcacccaagc gggttcgttg atttgccgcc
    21301 cgagcgatgg ttccatcgag cgcatccgtg acgccggcca ccagcaggac gatcatggcc
    21361 ccgttgaacc gttcgtaaat caataacccg acgtagaccg gaatcagcaa aatgcgaaga
    21421 atggtcagac tgttggggat attcatgcgg cgtaggaagc atccgacccc ggctgagcag
    21481 cgaaataata ggaggcgtga gcaatgaagt caacggcttg cagtcggcgg tcgatcgccc
    21541 tatactgcaa ctccgcctac acgaggaaat accgatgaac gtgctggcct cgctgtttaa
    21601 acgagacgga ttagtcgaaa aacaccagct ggaaggggtt gatcccagcg atcgttattt
    21661 caaccgagcc gttctcgtca accgcaccgc ttccggctac tcggccaaga tgatgtacga
    21721 ggcactcacg gtcgaaggcc tatcgcatcc caccattgcc gccgccatca cggagcttgt
    21781 tgtaaaattg cagtcgcttg gcttcagccg gatcaggacc agggcaaatt tcaaaggcac
    21841 taagtatctc gcagaaaaag aaacctggat cgaatatcag gatccggctt gattcacttc
    21901 gtcccgctca cgatttcctg aaacaccaca tcatgaatca gcggacggaa ggcccggatc
    21961 ttttcattct tccgggttgg atgtacgcga ttggtgagta acagcacttc caattttctc
    22021 cggggatcaa cccataacga ggtccccgta tatcccagat ggccaaacga ttccggagat
    22081 aaatggctcc cggatgacga aggcgccgtc ggcgtatccc atcccaatgc ccaactggac
    22141 tgcgaacaag atgacgccct tcgcgtgaac tcgcgaacga gtctctgctc aagcatggac
    22201 cgctgtcctt cataggagcg aagccagaca tcggagacgg ccagcaccgc ttccgcggtg
    22261 ccgaacaacc ctgcgtgccc tgcgaccccg cccatggcag cggcattctc gtcgtgcact
    22321 tgccctttca gcagttgccg cctccactca tcccattccg tcggggcgat gctcgagaca
    22381 gccaccgccg gtcgactcga acaggactcc gtctcagtgg gattgaagca cagaggctgc
    22441 gctcccagcg gccttgccac ggactcgtca aaatactcgt ccagcggttt tccactcgcc
    22501 cgttcgaccg ctaaccccaa cagaataaat ccaaggtcgc tgtactcact ccgctcgcct
    22561 ctccggtact ttaatggttc ttggccgatg aggtgcaaca ccgtttgtcg tacggattca
    22621 gatcggcccc attcgatgcc gttggctgtg agtccctcat acagcggacg ccatcccgga
    22681 agaccagacc gatgctggag aaggtcccgt agcgtggcta ccccgacggg cgtgccatga
    22741 aggtcgtcga gagtgtgttc tacgggtgcg tcgaggtcca gcgttcctga ctgcacaagg
    22801 aggagaatgg aggtcacggt cgccaacggt ttggtcagcg acgccaagtc ataaaccgtg
    22861 gacgcctgca caagatcggt tggatctgac gagagacaac cggacacgac aagacacttg
    22921 agctcaccgg ccaaccgcac ggccaacacc ccgccgggga atacgccgtc ccgcaccgcc
    22981 tgatccattg cggcctgaat gacacgagag cctttcatag acaacggcca caggcgatgg
    23041 aagaagcgac ttgaaagaac cgtgcatcgc tccacgtgga ccggaggctc aaaagcaatg
    23101 cgctaggtat tcgcacgggg ataggttcga aaacaggtga agaaggaagg gcgcagagga
    23161 aggatcgctt taccatcctc tcacaatgca gggctcgatt ctccctcgag cttatgctcg
    23221 gcactcgagg caccgtcttg gaaggcttct tgttcctcca cttccagcat ccgctcgaag
    23281 gtgcgcaacg tcgcacgcaa gcgttcgacc attaagagcc gctgcttttg cagaacagag
    23341 agatcacgct gagtgttgga cagctcgacc cgagcctgac gtatcatctc gccggccttg
    23401 agctcagctt ctttgatgac gagctcggac tcccgttgcg cgctgcgttt gacatcctct
    23461 gcgagcgact gcgcagacac aagcgtgttc gacagggtca cctcggtccg ctttaattcg
    23521 atcaactgct gctccaaaaa cgtgattttc tctctctgca ccgcattctc acggttcagc
    23581 tcttcgaccg tctcggcaat ttcttccaaa aaacgattga cttcttcctt gtcgtatccg
    23641 cggaagccgg ttttgaacac catttgctga atgtccagcg gtgtgatttt catgactgtc
    23701 ctcctctagg atgcttatcc aatcgccgtt gccatttgat gaagagaagg cacaataaat
    23761 ctctgtaaaa acatgagaag aaggatcagg ataatcggag acacatctac gccaagcctc
    23821 cacccgatca tccttcggat gggcgccaac gctggttcag tcactttttc aagaaactgc
    23881 acgatcggat tccacggatc agggttgacc caggaaatca ccgcacgggc gatgacgacg
    23941 tacatataca actccaagag ataatagacg attgaagcca atgccgtgaa aaaattttcg
    24001 acgacaaaca tcagcgcgcg agctcctgcg aacgttttgt cgcggcttcg acggccccca
    24061 tcagtgtagc acggaacttg ccctgttcaa gctgatggag cccggcgatc gtcgtgccgc
    24121 cgggcgaggc gacacggtcc ttcagtgttg cgggatgggt gccggtctca agaaccattc
    24181 ttgctgcgcc cagcactgtt tgaacggcca ataactctgc cgtttgacga ggtaatccca
    24241 tcttgactcc gccgtctgcc aaggcttcaa ggactaggaa gacataggca gggccgcttc
    24301 cactgagtcc tgtcacggca tccatgaggc gctcttccac gggaatgacg cgcccgacgg
    24361 cttcgaatat cgtgcgggcg acgagactgt cttcttccct cacttcctca ccccatgcca
    24421 aggccgtgat gccctcacgg accagggcgg gcgtattagg cattgcgcgg atgatgcgcg
    24481 tacctgggga cgcctggcta gcaatggact gaatggtcac gccggcggca atggaaatga
    24541 ccagcacccg attgagatcg gcgccgatct cgctcagcac tggagggagc gattgaggct
    24601 tgacagccag gatcacaatg tccgcccatg tcacggcctg ccggttctct gcaccgaccc
    24661 gaattgaaaa cagactcttc aaccgatcac ggcgagcggc gaccgggtcg gacgcccaga
    24721 gcgcatccga gtcacagacg cctgcggcga gcaggccacc gatcatggcc tcggccatgt
    24781 ggccactgcc aataaaggca atttttttcg ccatctgact acccacgtcg ctctccgaac
    24841 agtgccgtgc caaggcgcac caaggtcgcc ccttcctcta tggcaacctc ataatcatgg
    24901 gacataccca ttgagagttc gtccattgag atccgagaaa tcttctgttg tcttaagcgt
    24961 gccgccaaat cccgaagtgc tcgaaaatag cgcctcgctc gctccggttc gcgcatgagc
    25021 ggtggaatcg ccattaatcc acacaccgcc acatgatcga gtgcccccag ttgaggaagg
    25081 acccctgcca cttcatcacc cagaaacccg gctttgctgt gttcttctcc gatattcact
    25141 tccagcagga tcttttgccg taatcctgcc gccctcgccc gccgatcgat ctcatgagcc
    25201 aattccaagc tgtcgacgga gtgaatcaga tcaaaaattc cgacgactga gcggaccttt
    25261 cgtcgctgga ggcggccgat aaagtgccag atgacgcccg tgtctttcaa cgcctcgatt
    25321 tttggaaggg cctcttggag ccggttctca ccggcaagtg tcagtcctgc atccactgcc
    25381 cttcgaatat ctccgattgc gactgatttc gtcgcgccga ccaattgtac actgccggcc
    25441 acgcgaccgg ccttgcgcgt cgccgcgcgg atttgattga agacgaatgc gacgcgattt
    25501 tcgattgagt caccggcttc ggcatccatc tttgcccgat caaacccacg gctccttgga
    25561 atgcgtcgcc gtattgtaca ccggcctacc agtggggcat caacgtgatt ccgttgagca
    25621 tggtgccatt cacgcggcct tcacgccgat aggagaagaa tagatcggga tggcagatcg
    25681 tgcagagatt gaccgtcgta atgtgctcac ggtcgatgcc gatttgggcg gcctgtcttt
    25741 tcaccaaggc tttcaagtcg agcttggctc ggttaccgcg acgatctttg aggagcgaag
    25801 accactcggt agagccgctg cgtaacggct cgagcaccgc ttcatcgacc tcatagcaac
    25861 aggatccggc ggacggaccg atactcacgc gcacatcggc ctcatcggtc gaaaagcggg
    25921 cgaccattaa ctggatggtc ttaggtagga tgccagccag cgcaccccgc catccggcat
    25981 ggattgctgc tgcaactttc cggcgccggt cgtagacaag tacgggaacg caatccgccg
    26041 tacgaactgc gaccgtcaca ccaggctgat ccgtgatcaa cgcgtcccat ccaccttcaa
    26101 atcggtcgga tccggtcagc ggacgatcaa cgatcagtgc atccgtgcca tgcacctgtt
    26161 tcacggagag catccagctc tgcccgctga catcacgagt ttgtgcaggc acaccggttt
    26221 catacacgag cgtcccggta tggtgccgcg ttccgaaaaa atgcctcacg ccgtcatgcg
    26281 gcaagccaaa ggcaggcacg gtgatcaccg aggcggtcac gctgctgctc cctcatccga
    26341 ctcaaaccgg aaagcttcgc tcagtcggcc tgtttcctca aaaacgtcgg cacgtcccac
    26401 tcgtcatcag ccgccacggc cagtcgctcc atggattcac ggtgatctcc catccggcgt
    26461 agataggtcg gacggtccaa atctttgtgc ggccgatcgg cgaccgccac accattgaaa
    26521 gacggctgcc cagttcgggc catgcgagct tggcttgcaa ctcccaccgg cgtcggctga
    26581 tcgtctcgct caaagccggt cgcaatcacg gtgacgacca gatcgtcgcc catgtcggga
    26641 ttgataacct gtccgacaat gatgttcgcc tcgggatccg cggtctgttg gatgatggac
    26701 gctgcctctt caatttcgtg cagggacata ttcggcccac cgctgatgtt cagaagcacg
    26761 ccgcgagcac cttccacact gccttcctcc agcagcgggc tgcaaatggc cttttgcgcc
    26821 gcttcgatcg cgcgattcgt cccccgagaa acacccatgc ccatgacagc gcggccggtg
    26881 tgagacatga cggtacgcac gtcggcaaag tccacgttga catggccggt cgtcgtgatg
    26941 acgtctgcga tgccttgaat ggcctggcgc aggacgtcgt cagcgacttt gaaggcgtcc
    27001 aacaaaggcg tcgccttgtc gacgatgccc agcaaacgtt ggttgggaat cacgagcaac
    27061 gtatcgacgt gacgccggag atcgcgaatg ccttcatcgg catgcgtcat tcttcgatgg
    27121 ccctcatacg aaaacggttt ggtgacgacg cccaccgtca gaatccccag ttcacgtgcg
    27181 atgctcgcga cgatgggtgc ggcccccgtt cccgttcctc ctcccattcc cgctgtcacg
    27241 aacaccatgt cggcgccttc caggcattct cgaatctgat ctttactctc aagcgccgat
    27301 tcttttccaa tttccggctt cgctccggct ccgagtcctc ttgtccgctc aggtccaagt
    27361 tgaaccttat acgcagcacg ggatcgatcc agcgcctgca ggtccgtatt cgcggcaatg
    27421 aaatcgacgc gggccaggcc cgacgtgatc atcgtattga ccgcattgca gcccgcgccc
    27481 cccactccga tgactttaat gcggacgggc gacagacaat cttcttggaa tgagaacatc
    27541 aggacacctc cattggcgcg aggaggtccg cgcgccggtt aagttagaag aactcgaaca
    27601 tccaggactt cattcgatct accatcttgc caattggccg cccgctcctg agtcccaccg
    27661 tggcggtttc ttcggtatgg cgtcgcgcgt gcaacagtaa tcccaccccg gttgcatgca
    27721 tgggattgct gacgatgtcc cgcaatccac caatgccggt tggcactccc cgacgggcgg
    27781 gaaggttcag cacatgctca gcggcatccg gcatgccttc cagtaaggaa gtgccgcccg
    27841 tcacgacgac tccggcgccc aacatccctt catagcccgc acgggtgatc tctcgcctga
    27901 ccaactcgaa catctcttcg acccgtggct cgaggatctc ggcaatatcg cgtcgcgaga
    27961 acgttcgtgc tgggcggtct ccgactgaag gcacttctac cgtttcatgg ccctgcacca
    28021 attccgtcct cgtcacacca tgctgcactt tgatcttctc ggcttctgtc tgggaggtga
    28081 gcaacccgat ggccaaatcc ttggtcaggt tttgtccccc gatcgggagc acggccgaat
    28141 gtcggatgct tccatccaga tatatggcga ggtcggtagt gccgcctccc agatccacca
    28201 tcgccacgcc gagatctcgc tcttcctggc tgagcaccgc ttcgctggag gcgagaggct
    28261 gcagaatgat gtcgacgaca tcgagccccg cacgattcac gctcttgatg atgttctgtg
    28321 ccgacgtgac ggcaccggtg atcacgtgga cattcacctc caaacgattg ccggaaagtc
    28381 ccaagggctc gcgaacgccc tcctggccat ccaccatgaa ttcccggggc agtacgtgaa
    28441 gaatccggcg ctcgtgagga atgacggcca acgttctggc gctttcgatc gcccgatgga
    28501 tgtcgtcgcg cgtcacctca gcttttttga gggcgacgac acctttgcag ttttcagcag
    28561 agatgtggct gccggcgatc cccgtgtaga cggaattgat ctgcaccgcc gccatcaatt
    28621 ccgcttcttc gaccgctttc ttgatggact ccacggtgct ctcgatatcg acgaccacgc
    28681 ccttccgaag tccgcgcgac ggactcgaac cgacgccaat gatgctcaaa gtcccttcct
    28741 cggtcacttc ggacacgatt gcgcagatct tcgtcgtccc aatatccaat cccacgagaa
    28801 tttggtcccg cttcgccaca gcctcacccc ctttccctga cgatcacccg gttgtcatag
    28861 cgcaaatcta cgtcgtgacc ctcatgcttc cgtccatcga gagccggcat cctgaacgcc
    28921 gctttcactt tcatgaagcg ctcccattga tccttgagcg catcgctacc gaagtgaaat
    28981 cgaactcctc tggctgaggc gacaagattt acggggtgag ccaggtcgag ctcgatccgt
    29041 ccatccaggg attcggccat ccatcttgcc agttcgaccg cggactgaag tcgctgcttg
    29101 atgccgagat caccttgcgc caggagcgtg ccgtccacgc ccgtcaagag cggtaaggtc
    29161 gcctcgtcgc gggtgccgag gcgggcgagg atgactcctt ccttatcgat gaggacctgt
    29221 tcggacccta gacggacaat ggctgcgggc gtccgttcaa cgaccgtcac gcgcagctca
    29281 tgcaatggca accgctcgat ggtcgcttcc ttgatccagg gatgggtccg gacacgctcg
    29341 gcgaggtaag acaggctgac ctgatattga gcgagccctt tcttcaaggc caaccggtcg
    29401 atcacctcct gcttggtgac ttgatgcgtc ccttccactg atacctgttt gatctcgagc
    29461 cctcggtgaa gcatcggtcc gagctgttgc gagagcatga cgatcatcca gcacacggca
    29521 actgctcccc ccagccatcc ggagaggcgg agggctcttc ggcaccgagt cagccgctcg
    29581 atctgtctgg cccgctgccg gtgatccatc gactgagccg ctcgtgggcc tttccattca
    29641 ttactgcgat ggccggctgc ttttgcgggg ggcttgcgcc taagaagtct catgccgcgt
    29701 gctctttatt tttcatcctg gcgagcgcgg attcgagaat ccgctccgtc aattcttcgt
    29761 agccaatgcc ggcctgaacc gcggccattg gcaaaaggct ggtttccgtc atgcctggta
    29821 cggtattgag ttccagcaca tacgggcgtc cgcggggcgt aatccgaaaa tccacgcggg
    29881 cagcccctgc acagcccaag acttcgaatg cccgcaacgc caagacgccg atttccttcg
    29941 tgacggcagc cggaagaggt gccgggcaga ggtattgggt cttccctttt tcatactttg
    30001 ctgagaaatc gtaaaaaccg cccggggcga cgatctccac ggccggaaga accaccggcg
    30061 tctcgttctc tcgccccagc agcgagacgg tcacttcatg gcctgggatg taggcttcca
    30121 ccatcgcttc ccgatcgtaa cggtgggcga gtgacagggc aggattccac tgcgcaggct
    30181 tcttcacaat ggtcacgccg atcgtggacc cctcggatgc cggcttcacg accacgggcc
    30241 accgaagttt ggtcgatcgc agagcctgcg tatgggaaat ccgctctccc cgcttgatga
    30301 ccatgcctgc cggaaccgga atggcatgcg ctgcaaggac cgtttttgtc gtcaccttgt
    30361 gcatgcccac ggcgctggct tgcacaccgg agcccgtgta gggaatgccc accgtttcca
    30421 aaaacccctg aatggatccg tcctctccgc ctggaccatg gagcgccagg aacgcaacgg
    30481 caatcttttg ctcctgcagg tcgcgactca acgtcggacc gacgtcgacg gtcactgcgt
    30541 catatcccaa gcggcagagt gcgccgtgga ccgctgcccc cgtccgcaaa gacacctcgc
    30601 gctcggatga ttgccccccc atcagcacgc cgatccttgc ttgggtgagc cgtcgtcgtt
    30661 ccgtcttcat ctgttgacca accgcattat tcagtcaggc ctcgcctacg atcttcaatt
    30721 cgaggtcaag ccgcacaccg gctttccgct tgattgcctg ccggactttg ccgattaatt
    30781 gaatgacgtc agccgcactc gcatgtccgc ggttgacgat gaagtttcca tgcttcatcg
    30841 atacctccgc atcaccgact cgagcgccct tcaaccctgc cgcttcgagc agccgacccg
    30901 ccgaatcacc aggaggattc ttgaacacgc agccggcact gggcatcgtg agcggctgcg
    30961 tatccttcct ataatgaaga taatctttga ccactcgttc gatttccgcg cgaagaccct
    31021 ggcgcaactg gagccatact ccaaccacga tgcccttggg cagcccggcc ctccggtact
    31081 gaaatggaat ggacgaagcc ggcagatcgc ggagctcgcc cttcatggtg atgagcctga
    31141 ccgccttcac ggcgtctttc atttcgccca accttgttcc tgcgttcatc accaaacaac
    31201 ctccgacggt cccgggaatg ccggccgccc attccaggcc ggacaatgac cgccggatgg
    31261 catgaccgat caaggttggc atccccacgc cgccttccgc atagaggacc gctcccggct
    31321 cctcatgtat cccgcgaagc ttcgacaaac tcaccacgat gccgcgaatc cctccgtctc
    31381 ggatgagaag attcgtcccg ccgagcacga atatgggaat gcgtgctgcg ctcgctcggc
    31441 tgacgagccg gcgaacatct tcgacgtcag ccggctccac aagcacatcg gcaggcccgc
    31501 cgatcttgaa ggaggtatag gcctccaacg acgcgttgaa gcggatgcga ccgcgaagat
    31561 ctgcgacgac ggcttccaaa gccttgcggt cgaatcggga tctcgtcctg ggtctcttcg
    31621 ttcgttgtcc atcggcgttc atcgcatcat gaagcagcgg ccagcttctc cagtaatgca
    31681 ggccctgtct tccagatgtc tcccgcccca agcgtgatca ccagatcacc ggctttcaac
    31741 tgaggaagca cttgatcggt caattggtcc ttccgctcga caaaggtgac cggcggatgg
    31801 ccgacggcac gaatcgcctc cgctaatctg gcgcccgata ctccgggaat cggtgactcg
    31861 ctggccgcat aaatctcggt caaaaagagg cgatcggcat cttgaaatgc cttggaaaaa
    31921 tcctccaata agtctctggt ccgcgtgtag cgatgcggct gaaagagcac gaccagccgc
    31981 cgctcgggcc acccttgttt tgcggctgcc agcgtcgctt tgatttcagt cgggtggtgg
    32041 ccatagtcgt cgacgaccat gaccccgttg gattctccgc gtatatgaaa tcgccgctcc
    32101 acaccggtaa aggcggccaa tgccttgcgg atcaaatcca ccggcacgtc gagctcgacg
    32161 ccgatggcga tcgccgccaa ggcgttcgat acgttgtgaa tacccggcac cgcgaggcga
    32221 aacggtccta ggttcctggt cctgaaaaac gcccggaatt ctgaaccgcc ttgtttggcc
    32281 acgatgtcgg tcgcacgaaa atcgggggtc acgccatccg actccgacaa gccgtacgtg
    32341 taataccgtt tgactatgtt ggggaacagc ccccgtagcc gctgatcatc ggcacacaac
    32401 accgccagcc cgtagaacgg gaccttattg atgaattcaa ggaagctttc ataaatacgt
    32461 tccatgctcc cgtagtgatc cagatgctca cgatccaggt tggtcacggc gatgatggtt
    32521 ggagacagtc gtaaaaacga tccgtcgctc tcatcggcct ccgccacaag cagctctccc
    32581 cgacccagcc gggcgtggct acccaaggca ttcactttgc ccccaatcac catcgtgggg
    32641 tccaagcctc cctgcgccag gacattcgcc accatggacg tcgtggtcgt ctttccatga
    32701 gcgccggcga tggccacgcc gaatttcagc cgcatgagtt cggcgagcat ttcggctcgc
    32761 gggatgaccg gaatctgttt agccttggcc gccgtcacct ccgggttttg cggcgaaacg
    32821 gcggacgaaa tcaccacaac ctgagcctcc ccgatctggg attcgtgatg gccgataaaa
    32881 atccggcctc ccaattcctc caaccgcctg gtggtatcgg aggcctgttg atcggagccg
    32941 gtcaccttgt agccgagggt cagcagcacc tcggcaatcc cactcatgcc cgagccgccg
    33001 atccccacca aatgaatgtg ctgtgttttc cgaaacatcg atgtccgcct cagaccacca
    33061 gctgcctgcg aggaggtgaa gccgttgcgt tgccctgtcc catcagatcc ctgctgctcc
    33121 aagagaatgg ttgacatgac gctggtctcc tatgagtgca tagcattcac ggacgatggc
    33181 ttcagcggcg tccaatcgcc tcaagccaac actcgcatct tccatcgctt tcatccgctg
    33241 tgggtcgtcg aggatttggg ccaccgaccg ggccaaagtc gcgccggtga ggtcggcctg
    33301 gggcaatacc accgctgccc ctgcagcttc catgaccttg gcattcttca tttgatgatc
    33361 ataaattgcg gtcggcaacg gaatgagaat ggccggcttg ccgcaagttg tcagctccgc
    33421 cactgtcatg gcgccggcac gcgctacaac gatatccgcg gcgttgatgg ccgctggcat
    33481 gtcgtacaca aacggcagga cagtggccgt gactccggcc cgccgatagg cttgactcac
    33541 ccgatcatgg tccgcctcgc ccgtctgatg cgtgacggtc atgtggggcc gttgtttgag
    33601 aagatccgga agaccctcga tcgccgcgct gttgagggct ttggcgcctt gactgccgcc
    33661 aaagaccaat acgtgatgac cgcctcgctg gcgctctctt ccaggcggac gacattgagt
    33721 gagaaactct ttccggaccg gtgtccctac gacacgtcct ttgcgttgat cgaagaagcg
    33781 cgccgccgat tcaaacgcga gaaacacgcg ctgaacgaat ggggcgaccg ctttattggc
    33841 cagaccagga tacgcattag gctccaaaat gacccggggg atgcgcttca atgcggctgc
    33901 caataccatc ataggactgg tataccctcc gaccccgatg acgagacgcg cctgtcgctg
    33961 gctcaagatc cgaccgcatt gccagagact gagcggcatg gcgcacaacc ccttcaacac
    34021 gtcagtgagt cctttgccca tcaccggctt cgcattaatt aagaccaact cgaatccctc
    34081 gtgagccagc actttggact cgagaccgcg tgacgtcccc acgaacaaga ctttcgtcat
    34141 cggatcgcgg tgcagaaatt cccgcgccag cgccacagcc ggatacagat gtcctcccgt
    34201 tcctccggcg gcaatgacga tggtcattct cagtccccac tacgtccgcc tttagatgcc
    34261 ccgccatgcc gatccctcga aatgctcaga aggatcccaa cgccgacgaa actcacgacg
    34321 agcgatgacc cgccgtagct gataaaaggc agcgtcagcc ccttggttgg cagcaacccg
    34381 gtcacaacgg cggcgttgac caatgcttgg atcccgatta acagggtgat gcccattccg
    34441 agatacttgc cgaacggcat ccgcgcccgc gcggcaatgt gaaaccctcg ccacacaaag
    34501 agcccgaaaa ggacaatgac cgcggctgta ccggctaagc cgagttcttc ccccacaagg
    34561 gccaacacga aatcggtgtg cgcttccggt agaaaaaaca atttctgctt cccttcgcct
    34621 aggccgacgc caaaagggcc gccgctgcca aatgcgagga aagattgggt gatctgaaat
    34681 ccggcgtcgg aagcgtcttt ccacggggcg agaaaggtca tcagccgctg ccgtcggtag
    34741 ctggagctca ggacaagtat gaagatcaca ggaattgcac agagaccaag gctgaccaga
    34801 tgacctatcc gcgcaccgcc gaggaacagc aaagacaccg taaccagtcc catgacgacg
    34861 acggtaccca gatccggctc cagcaacacg agcccgctga ggaccgccat cacgatcaac
    34921 gctggcagga gaccgctccg gaagagcgtc aacttgtccg ccttcttggc caggtaggtc
    34981 gccaggtaga tcaccgaaac cagcttcacc atttccgccg gttggatcga aatgggaccc
    35041 catcgaagcc agcgcctggc gcccttggcc gcgacgccca gcgaagggac caagaccatg
    35101 acgagcagga tcgccatact gatgaggata ggcatcgaaa gttttttcca cagcgggtaa
    35161 tcgatgcggg aggtcaaatg catgagcaag aagccgaagc ccagccaagc gatctgccgt
    35221 ttcaaaaaga actcgggatc ctggaatcga ttgccggcca caatcgcgct ggcactgaac
    35281 accatgacca gacccatgag ggcgagtgtc agcgtcacaa caagcagtac atgatccatc
    35341 gctacgcgtg actggcccga tcgttgtcct gtcgtcggcc aaggcaacga taacgttcct
    35401 gcagttcgag atcccattga tcagagtcgg gcctcctgat ttcgtccgct actccggtag
    35461 cgactgcacc gccgatttaa actggcgacc gcggtcctgg tagtcctgga acatgtcgaa
    35521 actcgcgcag gccggcgaca tcaagacgac atcaccggcc gccgcctcgc gagctgccca
    35581 ctctactgcg tcccgcaagg actccgcttc gcttatggtc gtcgcgccag tccaagccgc
    35641 cttcaacaac ggtgcggctt ccccaatcaa cacgacatgc ttgacccggc gccgaatggc
    35701 ttcggtaaga cgggaaaaat cgccgccctt gtcacgaccg cccgcgatca gccagatcgg
    35761 tcgctcgatt ccctcgagcg ccttgagagt ggcatccacg ttcgttccct tcgaatcgtt
    35821 cacaaagcgc accccacgcc gttcccttac aagctccaag gcatgctcca gccctgggaa
    35881 cgcggtcaca acacggctga tgatgtcgaa agggcatccc cataaggccg cataggtggc
    35941 cgcagccatg acattctcca cgttgtgatg tccgatgatc ttaaggtcgc tcttacgaca
    36001 gatgtgctgt cggcctcctg cgacggtcgt aacgatttcg tcgccgtcca gataggttcc
    36061 gccatcgaag ccggcgccga ttgactgtgt cctggaaaat ccgaggcgcc tggcctgcac
    36121 cgtactcttc aggttggcta ctctcgggtc atcgagattg aacagcgcga aatcgtcgct
    36181 cgtttgattt tcgaaaatcc gccgtttagc tgcgacgtat tcgtcaattg agtcataccg
    36241 gtcctgatga tcgaccgtga cgttcaagat cgccgccgcc cagggatgga accgatcgat
    36301 cgtctccaat tggaaactgg acacttcaac cgcgaggtac tcataagggt ccggcttgcc
    36361 ttgcttcttt gcctggagag tggtccaagc ggcttcgctg agcgcagtac ccaggtttcc
    36421 tccgacgaat gcacgcttgc cctgctcggc cagcatcttt ccgatcagcg tcacggtcgt
    36481 gctttttcca ttcgtccccg tcacggcaag gatcggcgcc gagagaaatc gggaggctag
    36541 ctcaagctcg ctgatgactt gaacgccccg tcgtcggacg cgctcgagtg cctccatacg
    36601 gtagggcaca ccgggactaa tgacgacgag atcggcgcgc tcgagcgaac gttcgtactg
    36661 ggagccgacc gacacctgga cggaattgcg atccactgtc cgaaggaccg tggccaattc
    36721 cacctcctcc tttcgatccg ctaccgtcac acgagctccg gcttccctga gtaggtgaca
    36781 ggcggcgact ccgcttcgtg cgaggccgag caccgtcacc tgcgctcctg acaagtccac
    36841 ttcttcgacc tccagcatgt ccctcaccgc agtttcaagg tgctcagact taagagcgcc
    36901 agaaggatcg cgatgatcca gagccgcacg acgacctttg gctcctccca tcccttcatt
    36961 tcaaaatgat gatggatcgg cgccatcaaa aatatccgtt tgccacgcga cttgaaggac
    37021 gccacttgaa aaatgacgga caccgcttcg atcacgaaca cgcctccaac catgaggagt
    37081 aataattcgt gtttgctaat gaccgccacg gtcccaagtg ccgcgcccag gggcagtgaa
    37141 ccgacgtcgc ccataaagac cgatgcggga taggtattga accagaggaa tccgaggctc
    37201 gatcccaaaa ttgcggcagt aaagacggcg atctcacctg cgccgtcaat atgagggatg
    37261 agcagataat ccgacatgat gcggtgcccg gcgacatagg cgacgatggt gtaggccaag
    37321 gatgcgatca tgatcggacc gatcgcgaga ccgtccagcc cgtcagtcaa gtttacggcg
    37381 ttcgaactgc cgacgatgac caggacgacg aagatgatgt agaaccaacc gaggtccggc
    37441 atgaaattct taaagaacgg gacgttcagc ctcgtggaat agctggggag gaaatacagt
    37501 gcgacggcaa tcaacagtgc cacggcaatc tgggcgctga atttctgtgt tggggaaagg
    37561 cctttggacc gtgccttgat aaatttcaaa taatcgtcca caaacccgat cgccccgaac
    37621 cctaacgtcg cgatcatcac gagccagaca tagggtttcg tgatatctgc ccacagcatc
    37681 gttgacagca ggacggcaaa gatgatgagg aggccgccca tggtcggtgt gccgctcttg
    37741 gccacgtgat gcttgggccc atcgtcgcga atctgttgcc cgagcttgat ctcttgcagc
    37801 ttgcggatga gccacggggt gagcacgaag gcaatgagaa atgccgtcac tgcagcataa
    37861 atgatccgaa aactcaggta ccgaaacaca ttaaggaagc catattcggt atgaagcgga
    37921 tatagaaggt tgtacagcat gcgtgccttg atctcgttcc gcccgcttct ttatgaggcc
    37981 tgtttggtga cggatttcat gccagtcaaa ccctgcacga tttgttccat cctcatgccc
    38041 cgtgacgcct ttacaagcac gacatctccc tggcgaatcg tcttcttcaa aaggtcggcc
    38101 gcggaggaag catcgggcag ctgatcgatt gcatgactgg acattcctgc ctcacgcgct
    38161 ccgaccgcta tctcgcgtcc caggtctccg cagacgatta atctggtgag cttgagcgcg
    38221 gcggcgaact gtccaacctc gcgatgcaga ggcagggcct gccgcccgag ctccagcata
    38281 tctcccagca cagccacacg tgcgcgcgca ggtgcccaat ccgcgaggac cttcaacgcg
    38341 gccttcatcg aggcaggatt tgcgttgtag caatcattga tgatgtggac accctggtgg
    38401 gtcatcactt gcgaacgcat ggcagcagga cggaatcgcg cgagaccctg cgcaatgagt
    38461 gcgccaggca ggttcaacga gaaacccacg gctgcagcgg ccaacgcatt ggtgacgttg
    38521 tgcgtaccgg gcactttaat cgtcacggac attggccggc ttcttccagg aagaatcagc
    38581 ccaaaggtcg taccggtacg gacatctgtc gacagatcgc tcgcccggac atctgccctc
    38641 tcggacagac cgaatgatat gacgcggcac tgggccctcg cagccagata gtcaaaatac
    38701 gcatcatcag cgttcaatac cgccgtcccg tcggctggca gcatgtcgag aagctcggcc
    38761 ttcgcttgcg ccgacccctc catgccgccg aagaattcca agtggtcggg gccaatattc
    38821 gtgatgagtc cgatggtcgg acgtacgatt tcacaaaggc gggtcgtttg ccctcggtga
    38881 tcgaccccca tttcgattgc agctgcctga tggcgggcgg tcaggcgtaa cagcgtgtgg
    38941 ggaacaccga tccggttgtt aaagttacct tcggttttca gcgtacgcca ccgctgacca
    39001 aggacgctgg caaccatgtc ttttgtggtc gtcttgccgt tactgcccgt gacggccacc
    39061 acaggtattc caaaccggca tcgatgatgg gtcgccagtt gttgatacgc aaacagcggg
    39121 tcccgaacgc cgaaaaggaa cgggtcatgg cgggaaatgc cgctcgatct ttgcgtctta
    39181 gcctcccagc gatacgtatc gtgaacgatg gcgcccgtcg ctccttttgc gagtacggcc
    39241 tgcacaaaat catgtccgtc gaaccgctcc cccttcagcg cgacgaagag atcgcctcgc
    39301 cgtattgatc tggaatcggt agaaatgttc cgaatacgtc gcctcatgga gagcgacgga
    39361 tctcccgcca acaccttgac gctgatgact tcgcgcagtt cttctaccgt caaaagcccc
    39421 atcgactcct ctcgccgttc agtgcttcct cccacatgaa gcttcacgcg caattcttcc
    39481 tcaactgctg gatggcttca cgcgcgactt cccgatcgtc gaaatgcatc tttttcgtgc
    39541 cgacgatctg gtagtcttca tggcctttgc ccgcgatcag caggatgtct ccgggccgag
    39601 ccagacccac ggccgttcga atggcttcgc gacggtcggc aaccatgtgg tactgcaccg
    39661 acggtctgcg ttgcagcgct tcgcaaactc cggcctccac ctcccgaagg atcgccagcg
    39721 gatcttctgt tctggggtta tccgatgtca gaatcacaac atcgctgaat tccacggcgg
    39781 cgcggcccat tttcggacgt ttcccgcggt ctcgatcacc accacatcca aatagggtga
    39841 taatccgctg tgtttttagc gtccgcgcgg ccgtcaagag ccgtaccagc gcgtcctcag
    39901 tgtgggcata atccaccacg acggtaaagt cttgtccggc agacacgcgc tcgaaccggc
    39961 caggaacgtt ggtcactcgg gctacggcct cccggacttg ctcgcaggtg gctcctcgat
    40021 gaagcgccac accgatcgcg ccgagtaagt tgtacacgtt gtgttcgccc actaagcgac
    40081 tttcaaccgt aaaggtgccg gcgggagtgg cagctgtaaa cgtggagccg ttcatcgaca
    40141 ggcgaacatt ctcggccacg agatcggccg agacattgag cgcgtagccc catacgggaa
    40201 cccggcaggc ggctcggaca tagacccctc gaggatcgtc catattgaca atggctcgct
    40261 gcctggtttt tttcccttgg ccaagccccg tgaacagccg cagcttggct tggaagtatt
    40321 cttccattgt gcggtggtaa tcgaggtgat cctgcgtcaa gttcgtgaag acggcgacgt
    40381 cgtactcaca tcctgcggtt cggtccatgg ccagcgcgtg cgaggacacc tccatgacga
    40441 cgctatccaa ctgcgcttca ttcatcctgg ccagcaatcg ctgtaattcc accgcaccag
    40501 gagtcgtgtg cgacgccggg atcgtttccg ctccgatctg ataggcgacg gtcccgatta
    40561 acccgacccg acgaccgatg ttctccaata gcgccttgca caaataggtc gttgtcgttt
    40621 ttccattcgt tccggttaca ccgatcatcg ctagctgcgc cgagggatca ccgtaaaagc
    40681 ggctcccaat caaacccaat gctttgcgtg aatccgtcac ctgcacaaac ggaaccgatg
    40741 ccgtttctac gaaatcttgt ccgacaatcg ccgcggcgcc ggctttaatc gcttggccga
    40801 catacttgtg accatccacc ctctcacctt tgaccgcgac gaagaggctc cccgaggaaa
    40861 cggacctcga atcgtccgtc acggaggtaa tcgagccgtc caggtccccc agacggtcaa
    40921 caatggccac ttgaccgttc aacgccgcca acaattgcgt gaccgtcagc gctcgaggct
    40981 gcatcgtcac cttcactgcg gtgcgaccat ggcaatcttt acctggtcgt ctcgcgatac
    41041 accgagatag gtaagcacct gttctccgac acggcgaaat accggagccg ccacgacacc
    41101 accccaggct tcgccttgcg gttcatcgat cacgacgatc atcgccagcc ttggagaatc
    41161 agccggcacg aaaccgacaa aagacccgac ggagagtgcg ctggaatagg ctccagtacg
    41221 tggatccact ttctgtgctg tcccggtttt gccggccaca cgaaatccgg gaattgccgc
    41281 tttcgtgccc gtcccgctcg tgactacgcc ttccaagatc gtcgtcaaag tccgagcggt
    41341 gacgggggag accacgcggc gcttgacgtg aggaaaagtc tccttcacga gctgtccttt
    41401 ctgatctctc atccctgaca ccacataggg cttcatgaga acgccgtcat tggcgatggc
    41461 ggcgacggcg gacaccattt gtaaaggcgt cacgccgact tcctggccca tggaaatcga
    41521 cgcgagggag cgccggcccc actcacgcgg agacttcagc aatccagcca cttcacccgg
    41581 aagatcgaca tccgtgcgct gcccaaaccc gaacgcttgg aggtagcgat acagccgtga
    41641 ttcaccgagc agcatgcccg tttttgccgc tccgatattg ctggactttt gaatcatctc
    41701 ggcgaaggtc atccagccga gcttttcgtg gtcatgaata gtcgtactgg cgacggtcat
    41761 cctgccgttt tcgccgaaca acatcgagcc gggcatcatg actcgctcct cgagagcggc
    41821 agccgctacc accaccttca tcgtcgagcc aggctcatag gtgtctgtca aagcccgatt
    41881 ccgccagcga tcggcggcca gcgccgctac cgcatttgga tcaaatcttg gactgacggc
    41941 catagcaagg atcgctccgc tttggggctc caggacgatg atggttcccg atttcgcacg
    42001 agaatggtca acagcctgct ccaactcttt ttcggcgatg tattgaatga cctcgtcaat
    42061 tgtcagcgtg agcgcatgcc ctgcggcagg cacttgttct cggagccctt tgggaaacac
    42121 ggttcgtccc agcgcatcgc gctggagaat cgtcatccgt ttttccccgt gcaactgcgt
    42181 ttcgtaccga cgttccaaac cttcaaggcc gatgccatcc atcccgacga accccagaac
    42241 atgggcaagt agtggaccct ttggataaaa ccgccgccct tccatcacca tcccaatgcc
    42301 gtcgattgac agctgctcca gtcgatgtcc ctgctcgggc tcgatctttc tggccagcca
    42361 cacaaaatgt ttctcctggc gaagtttctt ttcgatttct tcgcggcgaa tgtgcaggac
    42421 cggggacagg aatcgagcgg cgttggctgg attgtcgagc gatgtcggga cgccaaagat
    42481 ggacggcact tctacgttca tcgccagcac ttttccgtgt cggtcgacca ccgttcctct
    42541 cgcgccttct aatgtgacgc ttttttgatg ttgccgatcc gccctcgctg tcagctcagc
    42601 cgcctgcaag acttgcagcg tgacaagccg gaataagatg atgccgaatc ccgccagcat
    42661 caggactaac aaggaatacc ggcggccccg ggatggaccc atcttatcgt tctccttttg
    42721 ccgtcgagat cgtgcgagcg atgcgcacct gctcaaggga cggaggcgga gcctgctgcg
    42781 gatggacgat taacacctgt ccttgctgcg gcggcaccat tcccagttta tctgtggcaa
    42841 ctttcgcaat gcgttccggt gccgtcaggg cggaaaactt cacttgcagc tggtctcgct
    42901 cacgttccag caacaccttc tgcgctttca agcgctcgat gtgatatccc aaccgcacga
    42961 tatccacgcg ctcccagaca aagaaaaaca cgaggcagag accggccgtt atgacgacgg
    43021 atttcatgcg tgcccctcat gtgcgatccg ttgcagaact cgaagcttgg cactgcggga
    43081 tcgaggattt cggcgcatct catcttcggt gggcacttgt ggccgcttcg tcaggacttc
    43141 gaactcgccg gggttccgaa tggaaaaggc cttgaaggac tgtttcacaa tgcggtcttc
    43201 gagagaatga aacgagatga cgcagagccg tcccccaggg gccaagacat ccgctgcatc
    43261 acggaacgac gcttcgaggt gatccaactc attattgacg gcgatgcgaa gcgcctggaa
    43321 agtacgcgtc gcacaatgaa tgcgtccgcg gcgataatgt cctggcactg cggattcaat
    43381 tacagatacc aattcccgag tcgtcgaaat cgtccgttgc tgccgagcgc gaacgagtgc
    43441 tcgagcaatc cgccgcgaat atcgctcctc accccagcgg taaatcatgt ccgccaaccc
    43501 gatctcatcg agtccattta cgatatcagc cgccgtcgga ccactgcgat ccatgcgcat
    43561 atccaacggc ccctctgatt gaaaactaaa accacgggcc ggatcgtcga gctgtggaga
    43621 cgacgcaccg agatcgaaca gaactccctc cacatgtgaa atgttgtgct cgacgagaag
    43681 cgccttcagt tccatgaaat gtcccttgat gatgactgcc cggtcgccga acggctgcag
    43741 tcgccgccga gccgcatcaa cagcctccgg gtcgcgatca atgccgaaga ctttgccgtc
    43801 cggatcactg gcttcaagga tgtcaagggc gtgcccacaa taacccacgg tgcagtccag
    43861 gtagatcccc tgactacggg gcctgagcca gaagaggact tctgcgggca taaccgggat
    43921 gtgctctcgg ttgccgccaa actgacttcc accaatttca cccacattct cccacgccat
    43981 cgcggagtat actcttcact tttacattgt caacaataat ttcatggact tatggatatc
    44041 gtttccagca ctgatcagcg gacgatgtat accgcttgac cccctcgagc aggggcagat
    44101 atccacagcg gaaacgtgga gattttaggg actgtccgtt tttacgacca ctaggcgttg
    44161 tgctggactt gaaaggttcg agcgcggagg gcgctccggg caagcgacaa atcagggata
    44221 gagcttatac agcatgcgag ggaaggggat ggtttcgcga acgtgttcca ggccgcagat
    44281 ccaggcaacc gcgcgttcga tgcccatccc gaagcccgca tgcggcacgg aaccgaactc
    44341 gcgcagatcc aggtaccatt tgaacgcttc ttccggaagc cgatgctctt gtaaacgcgt
    44401 ccgcagcaat tccagcgtgt ggatccgttg cccgccgcca ataatctcac cgtatccttc
    44461 aggcgccaac acgtccatac agagagcaag atcgggccga tgatcatcag gctgcatgta
    44521 aaaggctttg agtgcggccg gatagcgatg gatgatcacc ggtcgatcga attcctgcga
    44581 cagtatggtt tcttcgtcgc cgccgaaatc atcacccatc tgcgtgggat tattcttctt
    44641 gcgtagaata ccgactgctt cttcatacgt gagacgtgga aacggcggca ccacacgctc
    44701 aagcttacta atatctcgtt ctaacgttgt cagttctgtt cttctggttt tcaatacttg
    44761 ctgcacgatt gcggcaagga attcttcggc caagtccatc atgtctgcca actgtgcgaa
    44821 ggcgacttca ggctccacca tccaaaattc catcaaatgt cgccgcgtct tcgatttctc
    44881 tgcgcggaac gtcggcccga aacaatacac cttaccgaaa gcagcagccg tggcttcact
    44941 atagagctgt ccgctctgtg tcaaataggc cgtttcgtca aaatactgcg tctgaaagag
    45001 cgtggtcgtg ccttcacagg cattcggcgt gaaaattggt gcatccacca aaacgaaacc
    45061 gcgttcgtcg aaaaaatttc ggcacgcacg gataatctcg tgacggatgc ggagaatggc
    45121 atgttgtcga tgtgaacgca gccagagatg ccggtgttcc atgagaaatc cgacgccatg
    45181 ctccttcggt tggattggga acggcccggc aatttggagc gcttctaggc tgcgcacgtc
    45241 aagttcatac cctcccggtg cgcgatggtc ttgccggagc tgcccggtca ctatcagtga
    45301 ggattcttgt gtcaaactcg ccgacagtgc gaattgatca tcaccgacgg ctttcttgct
    45361 gacgaccgcc tgcatgtctc cggttccgtc ccgaagagtc aagaaatgaa gcttgccgct
    45421 gtcacgacgg ctgcgcaacc agcctcgaag agtgacttcc tgtcctacat gctgtgccgc
    45481 ggcatctatc gtcatcacag gcattgcctg tcctcctcag aaaaattgaa ttcgactcgg
    45541 gcagactagc cgatttgtcc gggctcgttc aagaagagca acattcctcg tttcattgac
    45601 aatatgtagc cgtgagagaa gaatggaaac atgtatccag gtcggcatct gggcagtcat
    45661 tttgcgatgg ctatcgtggc ggtaagcgcc acttacggcg cactttcgct ctccagcgcc
    45721 ttagccgcct cggccattta ctattgcgca gatcgacggg cggatcagct tatctcggcg
    45781 acacccggac caggctgcgt cccgcttgca gacacgaagc tggaaccacg agatgaatct
    45841 atccaaggga aggggaaaca ggattttcac gtcgagaatc ttgagcagga cgtttcggcg
    45901 ttcctgaagc gatatcggca cttcctggaa tgctgcaaga acgacctcag cgaattgagc
    45961 cacgtcgaag agctcgggga cgaagtgaac gaattgctga tctcggttca gggtaatctg
    46021 tccaacaatg cgctcgcatc aagagcgatc atgctgcggg agctgatccc gcgagtcgcc
    46081 aaggccagaa gcgacctaaa aacactgcgc gcaacgctgg aaaagatcag tgaactctcc
    46141 gccacacggg accagcttga ctttgaagaa gcgggacgtc aatcgcaacg aatccgagaa
    46201 ctcgaggagt cgattgaacg ggaaatccat gccccgaagt tgcccggaag cgccaagacc
    46261 ggagccaata tcggggttgc ccccgccgcg ggccctgcga tcggccgcag ctcgaaaacc
    46321 ggcacggaca tcggaggtat cggatcgagc gggcaggata tcggagcttc accccgaagc
    46381 agtcgagaaa tcggacagag cggcccgtcg ggatttgaga tcggcgcgac aggccgcgcc
    46441 ggtccggcga ttggacagtc ctcgcttaac caagagacgt cgtccgacgt tggttcgacg
    46501 ttgcagcggt cgaccgtcgg ctcgaccatc tccgattcaa ccgtcggatc cagtatcaat
    46561 tcatccacca tcggctccga cctccgcgac gcctcaggca gttcgtctcc ggagaattca
    46621 gcgagcaggc ctggcgagaa cacccgttag tgactacggg gtgagaggcg gcatgggtgt
    46681 ccaactctcc cccgcatccc gactccggta aaggccgctg ccattcgttc ccgcatagag
    46741 gaggtcgggg tcgatcgggc tttggacaag tgagcggacg ttggttgtag taaggccgtt
    46801 attgagcgtc ttccaggtca tccctccatc atcactacga tgcacgccat ctcgaccggc
    46861 aagatacacg actcctttct tcgtgcgatc cagaatcatc gacataatca tctgatccga
    46921 tagcaattcg ccaattcggg accaagacct cgctccatcc gtcgtcttga atacaccgga
    46981 aagcgttgcc gcatacacgg tgtctggcgc aaaccgatca acctcgatgg atgtaacatt
    47041 gagtgcccgt gatgtcttga ccatttctgg aggcacgagg ccgttattca ccttttccca
    47101 atgggttcct tcatttaccg ttttgtacac gccaccgctc gtcccggcat ataagattga
    47161 tggacgcgta gggtccatcc cgagcgtcac taccatcagg acctctttca tgccctccat
    47221 tctcttggcc caactgccgc cgccgctctt cgtctcaaat atccccatgg tcgtcgccac
    47281 gaaaatatgg ttgctatcga aggggtcaaa gacgaattgg ttgacgaccg atgagatggt
    47341 cgcatcatcc aaacctgagc gcatggaggt ccagcgctgt cctccgtcgt gacttttgta
    47401 gacggcgtct ccctttgtcc cggcatagac cgtcgccgga taagatggat ccaccgccat
    47461 ggatatcacg cgggaatgac tcatcccttt tgaaatattc gcccatgtct gaccgccgtc
    47521 gcgcgactta taaatatagt cgttggtcgt cacgtagata atgtcggggt tcttcgggtg
    47581 aagctggata acaacgattg gctcgttccg gttgcaggca acaagcaaga tgggaagaaa
    47641 cagccacgcc acagtcaatc gcttcatgaa ccgattgcgg cgtgtggtgg cgccactgat
    47701 catcgatagt tcgtaaactg aaggtcgatt ccaaaatcgc gtccacgaag caacgcaatc
    47761 gccgattgca gctcgtcttt gctcttactg aggatccgaa cctgctcacc ttgtatctgg
    47821 gcctgagcct tcagttttga atccttgatc gtcttcgcaa tttctttcgc cttctctgaa
    47881 gccaagccgc tttgaatctt cgcggtttgc cgtacggtcc cgctcagcgc cggttcgatg
    47941 tgaccataat cgaaggcctt tagcgagacg ccgcgcttca cgcattttcc tttgataatg
    48001 tcctgaactg ccttcaattt atattcgtca tctgagagga ccacgagttc cttttccttc
    48061 tctttcaagg tgatttccgt cttcgaatcc ttaaaatcga aacgttgctt gatttctttt
    48121 gtcgcctgat cgagtgcatt tttcagctct tgcatgttca cttctgagac cacatcgaac
    48181 gagaattggt cggccacctt cgcctcctct tacaccacgg tcagcagcat cctgttcctt
    48241 tgggcaaccg gaacccgcca tcatgctcgt catgcaccat cgcaggcagg ctcccagccg
    48301 aactgccccc cgtcattacg acgatctcag tgcttgtgaa cggttgcttc aacacgacat
    48361 atccgtacca ctgcctcatg ctgtcgctca acacaatcaa gcccaacagc gcaactaccg
    48421 caacgagaac cgcgtcgagg tagagcgcga ccgcatcctc ggccgccgat gccgcggcct
    48481 tccgcaagaa catccagaac atttcgtacg accccgtcag cgtcactaca ccgataaaca
    48541 acatcggcag cgccgtgatc cacagatagg aggacttccg catcttgatc aacaccgtcg
    48601 tgccgatgca aagtgcaagc gttcccagga gttgattcgc ggcaccgaac atcggccaga
    48661 tggtc
//
LOCUS       DASSCQ010000147        41289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1500951, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000147 DASSCQ010000000
VERSION     DASSCQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 41289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41289
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500951"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..833
                     /locus_tag="VFH05_10230"
     CDS             90..833
                     /locus_tag="VFH05_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678638.1"
                     /translation="MISAPEFSMSLGARPPRRTRWVLFLCAVLVGSVGCASSHGLSRD
                     SLHDVILREEAGFLDPPSAAAAPAPRHAPTLGFYFKPTAFVHREFEWTSRDRDAVLTW
                     AQGLSLGEGGRSTGFLTPSSLKGQSLRELRATAARYGVDWVLVFDGAAAVDRYNNYKA
                     PLLYWTIVGAYLAGGTHSDALCLMKATVWDVKTGARLFDEHADAITRTVGPAALVDDG
                     REIERAKTRAMETLLQRLTDRFALLVGRR"
     gene            833..2014
                     /locus_tag="VFH05_10235"
     CDS             833..2014
                     /locus_tag="VFH05_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6678639.1"
                     /translation="MPPIDACNAVAVRTVFLLSLITPVCSMAQTPVSEPPPVEKSSDW
                     HLSAYADLAYLLDFNFPENHLWRSRSTSQRVNELAPNMGFLNLTKEMTTDSRWGMEVG
                     IQGGYDAKTFGFLQDEPRVGGSDTLRHIQRANVSYLAPVGNGLSIQAGLFNSLIGYES
                     FYAKDNANYTRSWIADNTPYMMFGVNARYPVNDRLTIAGFIINGYYHLSRPNDQPSYG
                     TQIVYRPAADVTVTETLYAGPDQSKTAFEFWRFYLNSIVERKTDDFILAFSYDIGTES
                     IANQPGTPRTFVTGGNVVARWRIDGHWAVALRPEFYWDRNGRWTGHEQFLKAVTTTLE
                     YAVPFGWTRTRARLEYRWDESVGAGGGFYKGGDVAPGTPGLTASQHLLIMALLWSFYS
                     P"
     gene            complement(2037..2492)
                     /locus_tag="VFH05_10240"
     CDS             complement(2037..2492)
                     /locus_tag="VFH05_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HET6678640.1"
                     /translation="MRLTLARMTFVLLWSLSDAGHAAPSDGRFSLVMDGAAVKDNQTG
                     LIWEQEPDREHDVWSRSNERCAAKEVGGQKGWRGPSVDELKTLIDPAQRDPALPAGHP
                     FSNIKSEIYWTSTPDPKDDIVAWQISFYSGEPVTDQKSGTRRMWCVLAR"
     gene            2658..3257
                     /locus_tag="VFH05_10245"
     CDS             2658..3257
                     /locus_tag="VFH05_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HET6678641.1"
                     /translation="MQDEERRRRIDVLAIGLFGLAVGALTLGIAQVGAISESNKVGTL
                     VIALMFGGVVQILAGITDIRYHEQLGGTALTMYGFFWTTVCIAKLVSESTSFHLDMTL
                     YAPINFVYAAFSAVMVYLTAYRNVTLCMLHIVITVTFSSSVLVQLNLITETVPGLFHM
                     IVGFMAFYHAVASLTQAFTGRQLVPLGPPLLQQDVSMAR"
     gene            complement(3288..4247)
                     /locus_tag="VFH05_10250"
     CDS             complement(3288..4247)
                     /locus_tag="VFH05_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6678642.1"
                     /translation="MDHPLGNLKTAGPAGAVRTPVEQEAEMSGAEEESPFSRWTLPLL
                     LFLLTVFTTLWAGAYQAYSGTIRGPLVFLWEHPEMLWKGIPFAGTLLFILVTHEFGHF
                     ILSRIHRVPASLPLFIPGPPHFIGTFGAIIRMRGPILNRRALFDIGVAGPLAGFVVAV
                     VALVIGLFLSTVVDRTSTFGLQLGEPLLLKFMAWLIIGALPPEADVLLHPIAFAAWFG
                     LFVTSLNLIPIGQLDGGHVAYALWGEGQRTMAFAVLPLLLMLGFVGWPGWFVWAFMAG
                     MWGLAHPPVLDPQAPLGRGRILVGWIAFAVFVLTFAPIPFSFH"
     gene            complement(4258..5097)
                     /locus_tag="VFH05_10255"
     CDS             complement(4258..5097)
                     /locus_tag="VFH05_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6678643.1"
                     /translation="MPLEDDLCDIMKKARTGLKLSVGDVARLTGLPGADITALERGDQ
                     LRDRTELRAISTALGLRLAALEQIALEKWVPKTLPLMPGIETVLGEISGYGVKGYIVH
                     DSGEAVLVDTAYNADMMLEILEARRLRLVGICLTHGHADHAEGIHQIVSRWPVPIYLG
                     TEDVTLLHWKPSEQQRATPHDGRIIQVGRLAMTCLTTPGHTPGGVCYRLEMPWGPVAF
                     VGDTLFAGSIGRSNPPGLYATHLESVRRDVLTLPADCLLLPGHGPGTTVREELDHNPF
                     YVS"
     gene            complement(5116..6567)
                     /locus_tag="VFH05_10260"
     CDS             complement(5116..6567)
                     /locus_tag="VFH05_10260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6678644.1"
                     /translation="MMTRRTWVTTGAKLAVLVLGLVAAATTFAAGVTRDPRTMNFTPV
                     EFSPPEPARVVLENGMVVYLLEDHELPLVNVTATMRTGGWLEPQDKIGLAALTGSVMR
                     SGGGGSFTAEQIDEELEQYAIDIGISMSRQSGSASLDVLRKELGRGLRIFASLLRQPA
                     FEAGRVELAKLQMIEGIRRRQDSPGSIVGREFLKQLYGPDHPSARESTIDSVKRITRD
                     DLIAFHQRTVRPNGIILGVTGDFRQAEMLELLRQTFGDWKKGDVPDLSIADARESNGP
                     SIVRFVNKDTSQTHLRIGHLSIKEHDPDYVALAIANDILGGSSFRSRLFNDVRTKRGL
                     AYSVGSRLNVGMHDQGVWLMRAETKLPSTQEVISRFVANMERMRTELVTNEELAEAKE
                     AYVNSFVFSFASPSAIVGRFVELEYDGLPKDFLQQLRARVVALTREDVLAAAQKHFHP
                     DRLTIVAVGPGEALPKLLSSFGEVKEIKLIPEG"
     gene            complement(6564..8177)
                     /locus_tag="VFH05_10265"
     CDS             complement(6564..8177)
                     /locus_tag="VFH05_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6678645.1"
                     /translation="MNRARTNRARLAQPMGRVSRWLQVCCVAVVLGLASLSAAGSSGL
                     ADRVIEHKLANGLTVLLVERHQAPVVSINMTFGVGGVNEQVGQTGLAHLYEHMAFKGT
                     RTVGTKNYEKEKPILDELHQVGTELEQRQRELARRTADTPVDPAETRTIESLQKRFKE
                     LQDQAAQYVAGNEMALLYQRHGGVGLNASTGKDLTRYVISLPANRLPLWAAIESDRMA
                     SPVLREFYKERGVVMEERRLRNDDSPSGLLYETFTSAAFRAHPYGVPTIGWESDILSL
                     TPAVTEAFFKTYYGPGRATIGIVGDIDAKEVMTLIENTFGKVPAAPPPPQIVTVEPPQ
                     RGERRVEVEFDAEPSLAIGFHKPALGHPDDYVFDVIDELLTDGPTSRLYSKLVRDSRL
                     AASVNSDANYPGVRSANLFVLSATPLAPHSTAEVETAILAELERLKTEPVAPKELERI
                     VNNLDADMVRTLRSNSGLASQLALYQTVAGDWRFILKSRDKIAAVTPADIQRVATQYF
                     TKSNRTVATLVKKTASKQVATMTSDKGAR"
     gene            complement(8174..8821)
                     /locus_tag="VFH05_10270"
     CDS             complement(8174..8821)
                     /locus_tag="VFH05_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6678646.1"
                     /translation="MGKAPIDLLIFDLDGTLIESKWDIAASVNFTLEELGLPQRPLEE
                     IFGFVGDGVKKLLRLAVGETNRVSFEEALGVFRRHYLAHCLDRTVYYPGVEKVLGYFG
                     DKRKAVATNKSIEYTNVILQGLGPQYFNFVVGGDNGFGLKPEPGMLLHIMERLNTVPE
                     RTVLVGDSTNDINGGHNAGIRVCAVGYGMGNRERMSACQPDWFIERPEELMEIFE"
     gene            complement(8830..9363)
                     /locus_tag="VFH05_10275"
     CDS             complement(8830..9363)
                     /locus_tag="VFH05_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678647.1"
                     /translation="MAIMWMLSVLAWFNGAALAQTGGLSHSPAEIVKKYFELDQKGAK
                     LDSMSFEALAPYRSWQAEPAWGKVIVIRGFSVPEHHRHWEIVNPLEVVIPVTFQVIGS
                     VYLETAGFLPDDTVEEIRVRVKSIRNRWRIIEPVLPPHVGQKRMINYVREAWVKETEP
                     AKRESLAVLQDELRKVK"
     gene            9603..10028
                     /locus_tag="VFH05_10280"
     CDS             9603..10028
                     /locus_tag="VFH05_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HET6678648.1"
                     /translation="MPSPITYWVLLLAVCAAAPCGSPSGSRAHALELATEVNEAVVMP
                     DRPSCVPRSVIERTAPPKAYDVLAALQQRSGVPLPGYIGGNEFRNRERRLPHGRYREY
                     DVNRKIPGRSRGAERLVIEQRSGKAYYTGDHYRTFIPLN"
     gene            10034..10570
                     /locus_tag="VFH05_10285"
     CDS             10034..10570
                     /locus_tag="VFH05_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HET6678649.1"
                     /translation="MASHTSPLSPTRHLQSTKPPWTILVVLKTGQRAEAAITIPAGYV
                     LRVVRGAKCRYTAGLFSEFATAMDFPDYFGHNWDALEECLTDLEWLPGKGYVLLATGA
                     EQILTADEEEFATFLEVLSDAGEAWASGQAGAEGKPAKPFHMVLAVSERDQSKLAHWG
                     IPQVTEIPSPRSSRKRRR"
     gene            complement(10589..10831)
                     /locus_tag="VFH05_10290"
     CDS             complement(10589..10831)
                     /locus_tag="VFH05_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678650.1"
                     /translation="MKIVCSWCRKEGKHEFVGEKAPFDDARETHGICLLHRDQIREHW
                     QASTRYTGTSSFVRAATTFTLLRWSGLLNFTKNKRA"
     gene            11112..12395
                     /gene="hemL"
                     /locus_tag="VFH05_10295"
     CDS             11112..12395
                     /gene="hemL"
                     /locus_tag="VFH05_10295"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET6678651.1"
                     /translation="MKITRSIKLFEKAQHLIPGGVNSPVRAFRSVGGQPRFIKRAKGA
                     RLYDLDGNTYIDYVLSWGPMILGHASPTVVSAIKNAAERGTSYGAPTELEVELADQIR
                     GAFPSMEKSRLVSSGTEAVMSAIRVARGFTKRDGVLKFEGCYHGHSDYLLAKAGSGLT
                     TLGIPDSLGVPADFAKHTLTAPYNDIRTVQRILRDRGKDLACIIVEPVAGNMGVVPPA
                     PDFLPALRQLATDHDILLIFDEVISGFRVHYGGAQALYGVTPDLTVLGKIIGGGLPVG
                     AYGGRRDIMDLIAPAGPVYQAGTLSGNPLAVTAGLATLKQLRAKNLYKQLEERSAALA
                     RGLADEAKRAGIALTQTRVGSMLTSFFTQEPVVDWDSAKKADTKRYGLFFHGMLEQGI
                     YLAPSQFEAAFLSTAHTSADIEKTIRAARVAFKSL"
     gene            complement(12458..12688)
                     /locus_tag="VFH05_10300"
     CDS             complement(12458..12688)
                     /locus_tag="VFH05_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678652.1"
                     /translation="MEMFGRVGLVEIPILIAPDQKVWMDKMIKEGKIAIPPGGTLEKG
                     SLYSMFIRMLLHNAMEEQKRQEALEADDEDDE"
     gene            complement(13334..13726)
                     /locus_tag="VFH05_10305"
     CDS             complement(13334..13726)
                     /locus_tag="VFH05_10305"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HET6678653.1"
                     /translation="MEIKIYYEDTDCGGVVYYANYLKYFERARTDYLEARGLSVAALM
                     KQGCLFVVVHAEVDYRAPARYGDILDIATMIPEMSPASITFSHVVRERQSGRVIVEGS
                     ARLAVTDGNGKVRRLDKETVVALQFNHG"
     gene            complement(13850..15208)
                     /locus_tag="VFH05_10310"
     CDS             complement(13850..15208)
                     /locus_tag="VFH05_10310"
                     /inference="COORDINATES: protein motif:HMM:NF021207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HET6678654.1"
                     /translation="MKTLAGSLSAFQKTVKHVTTSREFDRCSCYSPLEMAQDQPVAAL
                     VVALKDDLPAVVYSLNRLRPECVCFVLPESAKALVETDVQPHISHMPRRWDWVPLSDV
                     DHFPSCYRTLARSLPPMLRAWEIGLGALVVDMSGATAAMAGALTLVTMPWTSRIASLV
                     EQREGREGDPAALEGGERLWISVNPWDEGAEVARREAFDFFNRGAFVAAGAIFRRIES
                     RVSGGQKPLYRALADLADGYDLWDRFQHRQAWEKLKVATKAIEMASLFGGPPGLNASL
                     PAVKANAGFLEKLVLDPAEVKELLAFDLLAHAGRKLRVANDAEAAMQSLVRALEAFSQ
                     RQLFKQYRIKTWDVQPEQLPQPMQDVCRSCYSDDIDGKYVLSLQAQFRVLAGLGDQMG
                     ESFLRQWPTMKPLFNAANRAVLGHGGEPVKAERVQQLFEVVMKVTHADENSLPKFPTL
                     TV"
     gene            15231..15665
                     /locus_tag="VFH05_10315"
     CDS             15231..15665
                     /locus_tag="VFH05_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET6678655.1"
                     /translation="MVWWYWMVLGLALLGAEMTTPGGFYILFFGLSALVVGTLAGLDM
                     VEAAWLQWLLFSMIAVGSLLIFRGPLLSRLRDNRRGDADIDSMVGEVAIPLETLAVGA
                     TGKAELRGTTWSAKNVGTTSFSKGQRGKVTKVEGLTLWIVAE"
     gene            15724..16662
                     /locus_tag="VFH05_10320"
     CDS             15724..16662
                     /locus_tag="VFH05_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HET6678656.1"
                     /translation="MSAGTWVVLVLALLVLYVIAKTAVVVPQQSAYVVERLGKYSDTL
                     NAGFHILLPFLDSIRYRHSLKETAVDIPEQVCITRDNVQVSVDGVLYLKVLNPERASY
                     GISDYHFALSQLAQTTLRSEIGKIELDRTFEERTNINIQVVNELDKASEPWGVKVLRY
                     EIKNITPPKDVLNAMEKQMRAEREKRALILTSEGERDAAINQAEGEKQQVIKASEAKK
                     QQQINEAEGAAAAILAIAQATAEGLRKVADTIQLPGGQEAVQLRVAEQYITKFGELAK
                     TNNTLILPASVSDVGSMIAVAMSAIRQTTPSASGKS"
     gene            16756..17334
                     /locus_tag="VFH05_10325"
     CDS             16756..17334
                     /locus_tag="VFH05_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HET6678657.1"
                     /translation="MKDLVVQAFTESAAVKQQFVREHADRIVEVAKLIVRTFREGHKV
                     LLFGNGGSATDAAHIAAEFVGRYKRERAPLPAIALATDVAAITCIANDYGYDELFARQ
                     VRAHGQKGDLAIAISTSGNSVNVLKGVEAAKACGMTTVAWTGGTGGKLAGLVDYPFVV
                     PSKVTARIQESHITLGHVLCELTEEELLGKAS"
     gene            17318..18310
                     /locus_tag="VFH05_10330"
     CDS             17318..18310
                     /locus_tag="VFH05_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678658.1"
                     /translation="MAKPPDRRGHSPSPRRLISPINVSDLKTYPLKKRHSKVRVSDFA
                     QPWRKGGSFSRFYRALPDILAVKTLRAVARAIAQAHRRGRPVIVGIGAHVIKVGLTPI
                     LVTWMEQGLVTAVAMNGAGIIHDFELALMGHTSEEVDDEIDDGRFGMTEETGRMLNEA
                     ITQGHRNGQGMGEAVGHYITRSKNQFPNRRTSILAMGATLGIPVTVHVAVGTDVIHMH
                     PAADGAAIGATSLLDFRRLAAVVAGMEGGVYLNIGSAVVLPEVFLKTVSLGRNLGHTL
                     SNITTVNMDFLSHYRPLTNVVRRPTQKGGKGYSLIGHHEIMLPLLVAAVAEELG"
     gene            18317..18979
                     /locus_tag="VFH05_10335"
     CDS             18317..18979
                     /locus_tag="VFH05_10335"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HET6678659.1"
                     /translation="MPGHAVPLSVSRITAIWRDLNTRYFDGALPPIAVVWSRRLTASV
                     GMFVSRAGPRSAWRANRSANSREIRLSLPLLTPLLTETPYGTQELISTLAHEMIHQWQ
                     FDVLKRRPNHGPDFLRKMTEMNRDGALAVTIYHSLKQEVLALSRFAWRCGRCGRIYRR
                     QRRTIQPRRHHCGSCRGALHELSSWEVAGRSSMETARLTAPCTSLQPARQLELDFSSA
                     SL"
     gene            19017..20174
                     /locus_tag="VFH05_10340"
     CDS             19017..20174
                     /locus_tag="VFH05_10340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET6678660.1"
                     /translation="MLRWSRQILFGDVDAMFASAAAVKDPTLTGKPIAVGGPPPRGII
                     AAASYAVRAFGVRSAMPTAEALRRCPHLLLIPPDRALYRRLHDDMRRVTDRLFPVTEW
                     GSIDEFYADTTDLQTRHPDPRLLAQLVKDSIFEATGLRCTIAVATGKTVAKIAADASK
                     PDGLAIVEPGREAAFLLPRPLRALPGIGPKTAARLEPFSIKTIGDLLDPRWDQVLARM
                     FGHRLLWIRELAQGRDREPVIGDRESKSSSHETTFERDTADPAFLEDVLRGFLRMLAR
                     DLRQEGLAAEGCTVKLKDARFAVTTRQRRFSHPLNYDPEMWPTVRQALHELMKPHAQY
                     RLAGLTLFSLVPIPAGLYDQRRRKAVQAMDTIIARHGAVIGLGGVCDDDNE"
     gene            20223..20705
                     /gene="rfaE2"
                     /locus_tag="VFH05_10345"
     CDS             20223..20705
                     /gene="rfaE2"
                     /locus_tag="VFH05_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET6678661.1"
                     /translation="MPGKVVTSAELVPFLKRARAQKQRVVFTNGCFDLMHVGHTRYLQ
                     AAKALGDILVVGVNSDASVRSLKKGAGRPIVGEAQRAEVVAALGAVDYVVIFPEPDPL
                     TLITALEPDVLVKGGDWTLDRIVGREVVEQRGGTVKTIPLVPGMSTTALIQRIRSQPH
                     "
     gene            20711..24181
                     /gene="mfd"
                     /locus_tag="VFH05_10350"
     CDS             20711..24181
                     /gene="mfd"
                     /locus_tag="VFH05_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET6678662.1"
                     /translation="MSHALSSSTTLPCCAPIAAALARKGARPCLTGLHGSTAGFALTQ
                     LMHPRAGVLGSEPWLIVTKTDEAAERLFEDLRFYHGLQGVPVTALALFPRWETLPYES
                     TAPHVGLIARRMNALHQIRSMPAAKVVTSIEAVMQRLMPVDTFQHTVLQFKVGAGIER
                     EVLTAGLLRLGYRRVSVVEIPGEFSIRGGIVDIFSTAYAEPLRVEFLGETVESLRLFD
                     PATQKSTAKMDRAMVLPAREYLKPDTAADALAPIAADAEWRAPDLYGQMHSLLDYFPT
                     QPILALDQPAALKQSCADLWERIDDGYLRHSDKEDVSYPSPERLFLTWQDIMDRTAEC
                     TQLALEPLAVAEGSWEPVQAFPGQVPASAGLGARGTPFSQTLSILEHLREACRVLLVA
                     RSRGQVDRLLALLREHDVPAIEWSTSSWSTSTDTRAPFFVLNGDLSAGFLSSELRLAI
                     LTEEELFAKGARHKPQAKSKAATFLSSLEDLNVGDYVVHVQHGIAKYRGLKRLSVQDF
                     DSDYLILEFYGSDTLYVPLDRLNQIQRYSGAEGHVPRLDRLGGTSWAKTTARVKKDIE
                     EMAQELVDLYANRELAKRHSYGHDSTLYHEFEAAFEYEETPDQLKAIEDITRDMEASK
                     PMDRLVCGDVGYGKTEVAMRAAFKAVEDNRQVAVLVPTTLLAHQHYDNFSERFAPFPT
                     RVALLSRFQSPKEAKTILKEVEAGIIDVVIGTHRLLQKDVRFRDLGLVIIDEEQWFGV
                     KHKERLKQLRAQVDVLTLTATPIPRTLQMAMASVRDLSIIDTPPAGRLSIRTQVVRSS
                     DKLIREAILRELGRGGQIYFVHNRVETMERTGAWLQQIVPEARVVMAHGQMDAKPLEA
                     VMLKFFRREADVLVASAIIQSGIDVPHANTIIVNRADTFGLAQLYQLRGRVGRSGDQA
                     YAYFLVPDEGRLTDDAQKRLTAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHIA
                     AIGLDLYMQMVEQAVQRLKGQIVEEEPDPTLRLNVSAFIPEDYVADPHHRLSLYKRLS
                     SSTQVGDLALLHGEIQDRFGPPPEPVERLFEVMQIRLQAKALRLVSIELKAGAVAVTL
                     DGKSRVPQTAIQQLMDRYKTRIRFLSPLAFELQMPHQDWASIFPELTATLQSLGVCDT
                     NTRATSPSPT"
     gene            24204..25106
                     /locus_tag="VFH05_10355"
     CDS             24204..25106
                     /locus_tag="VFH05_10355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6678663.1"
                     /translation="MHRRPTEYATASAVLHMCLMMMAVGGLLLPLVGCTPPQAEERVV
                     AFVNGKPITQTEFDHEWADLPDATKARYEKEGGRQVFLKELVDHEMLLQEARRQGLDL
                     DDAIRDRVRRYKEKLLIDELLKDRMKTTVELTKEELDRYYEQHASELLTPLKVRVAQM
                     LLPNYPAAKDLETQVNRGGDFGKFAQRYSIDYKTKAKGGDLGPYRKGLVIPEVDDAIR
                     TLKPGMISAPIKAESGYYLVMITALEPEIIQADLAKRERLRQELLYLKRKQYFDSVIA
                     EIKGKAVVRLADGSRFSGDGLTKR"
     gene            25284..26306
                     /locus_tag="VFH05_10360"
     CDS             25284..26306
                     /locus_tag="VFH05_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET6678664.1"
                     /translation="MSTSFRTATWLRLGISALLGLSSVGAPFPSFAAKVEDRIAAIVN
                     SDLIMLSEVRRELAPERERLEKHYRGEELSRRLQMAEAMALTTMIERRLQLQEAKARS
                     VEVGDQEVKQAVKQLIQQGEKIDDKDPVSTNSVRDQLTLLKVVDREVRSGVMVADPEM
                     KRYYQEHRDRFALPEEYTLSQILIKPRSPDEVADARAKGREVTALLKQGESFEDLALR
                     YSDGPTASRGGRLGLVRQGELLPAIERGVSNLVPGGISDIIESPEGLHIVRLDDKKPK
                     QFRPFEEVRQEIQALVFQQKSEDMFQSWLADLKNKAYIEIKFDSAPLRQTTSVPAPPP
                     SPTSKP"
     gene            complement(26230..26829)
                     /gene="yihA"
                     /locus_tag="VFH05_10365"
     CDS             complement(26230..26829)
                     /gene="yihA"
                     /locus_tag="VFH05_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET6678665.1"
                     /translation="MKILKADFIRSCVGAAQFPTDSIPEIAFVGRSNVGKSSLINSLL
                     QRKGLAKVSRTPGKTRAINLFAVVTDDPQLSRFVLADLPGYGYAKVSKTERAQWAPLI
                     EAYLGRDAQVCIVMLVESRIISPQDAETLLWLRAIGYEPVVVATKADKLKAGERVAAL
                     RRLQEGLGLSGSPTFMAYSSVTGEGRERLWSVLKEHCQT"
     gene            26864..27598
                     /gene="mtgA"
                     /locus_tag="VFH05_10370"
     CDS             26864..27598
                     /gene="mtgA"
                     /locus_tag="VFH05_10370"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HET6678666.1"
                     /translation="MRKSTKRRGRPLWLILCALVAIPCAALALLWLVTLPDVSSLRSV
                     NPTTTALIEARQIEAEAKGLRIGRRWIWVPLSRISPYLRRAVVAAEDASFFTHEGFDW
                     EGMKDAAIYDLEKGELKRGGSTITQQLAKNLYLSSERSLLRKAQEALITRSLEHELSK
                     ERILELYLNVAEWGTGVYGAEAASRHHFNKPARDLTEDEAAWLAAILPSPRRYDPLRK
                     THALAKRYERVLHRMAQNSRHQIRMD"
     gene            complement(27740..28180)
                     /locus_tag="VFH05_10375"
     CDS             complement(27740..28180)
                     /locus_tag="VFH05_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6678667.1"
                     /translation="MKDILFHLAFPIHDVNQAKQFYVEGLGCKLGRESAQAVTFGLAD
                     HQLVAHVVDGSPSEQRGIYPRHFGLVFLSKHDWQALADRAKAKGLRFFQQPRVRFPGT
                     RIEHHTFFLQDPSENLLEFKHYTHESAIFGERDFRAVGDASPAD"
     gene            complement(28177..29061)
                     /locus_tag="VFH05_10380"
     CDS             complement(28177..29061)
                     /locus_tag="VFH05_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HET6678668.1"
                     /translation="MTRPHPLLLAHGGAGRRPITRRQSSCLADALATGYAILEQGGPA
                     VVAVEVTIGFLEQSGLFNAGRGSKRQLDGIQRMDASLMEGAGLRAGAVASLEGFVHPI
                     TAARLVMEETTHVLLAGPLAGRFAQHFGLERHRPPGRRRQLGYDRILAGKGSVKDRHG
                     TVGAVALDRARTVAAGASTGGIEVMVPGRVGDTPLIGCGVYADNRCGAVSMTGQGEGI
                     IRLAVAKAICERLAAGKSPSTAARMVLRELVSRIAGAAGTLVVAPDGRFTIAHVTPHM
                     AAGWWNGSARPTVKGSWT"
     gene            complement(29058..29624)
                     /locus_tag="VFH05_10385"
     CDS             complement(29058..29624)
                     /locus_tag="VFH05_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678669.1"
                     /translation="MGTSIPARHPLRQLFGALTEKTFTEHLGWPDLNVTTYVTNLLVE
                     FTRTDQLFKIRNRQGQPVDSVVDLLFESEVLLEAQSLDRERDVHQHIGDFTLFMAGLF
                     PEYLRRLKTAGLVYHKDFLVDYVKTGKRSYGIVAQMADQPAQDERPLFAKLSDNFELC
                     VTGLGFVRSDLDRMKNPAYQQAKDLLLN"
     gene            complement(29688..30137)
                     /gene="dtd"
                     /locus_tag="VFH05_10390"
     CDS             complement(29688..30137)
                     /gene="dtd"
                     /locus_tag="VFH05_10390"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET6678670.1"
                     /translation="MKAVIQRVTRASVEVEGQVVGSIGAGLVVLLGVAKGDGDGDCRF
                     MVEKLRGLRIFADDAGKMNRSLTETGGGILLVSQFTLLGNTKSGRRPGFEDAAPPDEA
                     RHWYERVSKELKVQGTQVEMGVFAAHMKVELVNDGPVTFVLDSRNGF"
     gene            complement(30134..30664)
                     /locus_tag="VFH05_10395"
     CDS             complement(30134..30664)
                     /locus_tag="VFH05_10395"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET6678671.1"
                     /translation="MTRALIFKSSWGWMGVAESREGLAAIVLPQTSKVAVTTALGKGG
                     SGFNGTSSSALLREARKQLTEYLAGSRTSFDLPLDLSRGTAFQQRVWKKLGAIPYGQL
                     WSYRGLASRVGGVQYARAVGGAVGANPLPIVLPCHRIIAQDAGLGGFSCGLPAKRKLL
                     ALEGSLSRVRHADRDR"
     gene            complement(30674..32284)
                     /gene="murJ"
                     /locus_tag="VFH05_10400"
     CDS             complement(30674..32284)
                     /gene="murJ"
                     /locus_tag="VFH05_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET6678672.1"
                     /translation="MSDTATALSKTSQSENHSLVKAAGVIGVATFASRILGFIRDMVL
                     ARLFGATPAADAFYVAFRIPSLLRELFAEGSMSAAFIPVFTEYHSLKSKREAWELASA
                     VFTTLLTIVTLVTLLGILIAPWLVQALAPGFHASPEKLALTTVLARIMFPYLLFISLA
                     ALAMGILNSLRAFAVPAYAALFLNVCIIGCALFLSPQLEEPIVGVAIGVVAGGAAQFL
                     MQIPSLKARGFLFGIRFEPGHPGVKRIGRLMLPSLLGLSVTQVNLTISTILASFISGA
                     PTYLFYGMRLVQFPLGIFGVALAMAILPTLSAQAARGAMEELRTTFGFGLRMIVFIMF
                     PAMLGLICLRTPIVHVFFEHGTFTAHDTAETALALLCYAVGLWAFGGVRIIVAAFYSL
                     QDTTTPAISGAIAVAANIVFSLLLMSPLGAAGLALATALAGMVNGIILIALLNRRLGG
                     VAWGLVGRSAGRVLVACIPLVLSCLWVAGAQVWTHEGEWAAKSVMLTVAIGLSVSGYL
                     GVHALMRSEELDLLWSMVKKRFGRLIGR"
     gene            complement(32334..32996)
                     /locus_tag="VFH05_10405"
     CDS             complement(32334..32996)
                     /locus_tag="VFH05_10405"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET6678673.1"
                     /translation="MATGKAGPLTDEDKARISDAVRAAERLTSAEIVPMLVARSGLYH
                     DAQHRAGLALALMVLTGLLMSEAFWVPWGWHAANAAWLIVLTLMGYVIGSWLGTFDPV
                     IRAVTSTERLRSKVQLRAERAFAQHSLFRTRDRTAVLLMVSLMERHVYVLPDSGIGPR
                     ITAAQWNEVVEAVVTRLKADDIAGGFCAGIERCGVLLALAYPASSNDNPDELSNRLVQ
                     EP"
     gene            complement(32983..33864)
                     /locus_tag="VFH05_10410"
     CDS             complement(32983..33864)
                     /locus_tag="VFH05_10410"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET6678674.1"
                     /translation="MSRVWREPARLVVFLGFLLNPLLLSALEVPPLTGRIVDLAGALP
                     AHDSEQLSARLEAHEQQTGNQVAVLILPSLEGEPVEEYSHRVATTWRLGRKGTDNGVL
                     LLIALKERKLRIEVGYGLEGTLTDLRSAHIIRHEIVPRFKAGDIPAGVRAGTDAILRT
                     IEGTYQAAESPTRDRQLLQSSAFQYVVIGVFVGFLAGLVLSQGLRRARALLGAVLTFM
                     VAQAASILWGVAAAGLTAVLLWMALSAKGPGRRRGFDDWTWYRSSRGGWSGGSFDHGG
                     FSGGGGDFGGGGASGDW"
     gene            complement(33893..34483)
                     /locus_tag="VFH05_10415"
     CDS             complement(33893..34483)
                     /locus_tag="VFH05_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET6678675.1"
                     /translation="MGRVYMIVLGVLMTGGLWGCGYNDLQGLDEDTKAAWSEVVNQYQ
                     RRADLIPNLVATVKGYAAHEKETLEGVVQARAHATGIQVTPEMLKDPAAFEQFQKAQA
                     GLTSALGRLIAIAENYPNLKADQNFRDLQSQLEGTENRITVARKRYIDRVAEYNKMVR
                     YFPTNLTAKFILHLDERPNFSVADEKAVAKPPEVKF"
     gene            34560..34865
                     /locus_tag="VFH05_10420"
     CDS             34560..34865
                     /locus_tag="VFH05_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678676.1"
                     /translation="MSLERSSNQAGGFQLRHRVLGIYQGSAIELAFWHPSSGMPEYGL
                     CRFSTEDKAQALVDFLTSPACPEPFDPKDLTIEPYDLVEHERLTTDYPLPSAWEQPT"
     gene            complement(34925..35446)
                     /locus_tag="VFH05_10425"
     CDS             complement(34925..35446)
                     /locus_tag="VFH05_10425"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678677.1"
                     /translation="MGRSLNCPQCRHEQVLRARPLSASERVAAIFLLSPFSCQHCSCR
                     FLASRIGLDRPRHPIDRREHLRIPVRLYLSFSGGKVRGEGTVLDLSMGGCIIRSETQV
                     RVDDIFYLEIATGSHEQPLEVAAMVRSVSPRGIAFKFLRAAQENKRLLAFIQAQTADH
                     LQKSTALAVPVKS"
     gene            complement(35631..36452)
                     /locus_tag="VFH05_10430"
     CDS             complement(35631..36452)
                     /locus_tag="VFH05_10430"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET6678678.1"
                     /translation="MIEAEGNGRATMTFTLKIWRQNGPADKGRLVTYQVRDISPSMSF
                     IEMLDVVNQQLTLKDEEPVAFEYDCREGICGSCSLVIDGVPHGPDRGTVCQLYMRRFP
                     DGATITIEPWRAKAFPIIKDLVVDRSAFDRIMQAGGYISVNTGGAVDGNVLLIGKDQA
                     ETAMDAASCISCGACVAACKNASAMLFVAAKVSHLALLPQGQPERTKRAKAMVAAMDR
                     EGFGSCGNQYECEAVCPKSISVRFIAKLNREFLRATIEEPSPLAEQPADPHDESH"
     gene            complement(36486..38399)
                     /locus_tag="VFH05_10435"
     CDS             complement(36486..38399)
                     /locus_tag="VFH05_10435"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET6678679.1"
                     /translation="MITLDSKVPHGPLETKWDRHRFSEKLVSPTNKRKVGIIVVGSGL
                     AGASAASTLGQLGYQVDCFCFQDSPRRAHSIAAQGGINAAKNYQDDGDSVERLFYDTI
                     KGGDFRSREANVYRLAQVSMQIIDQCVALGVPFAREYGGQLANRSFGGVQVSRTFYCR
                     GQTGQQLLLGAYSALSWQIDRGQVRMHPRTEMLDLIVVDGHARGIVVRDLVTGAIRSH
                     TAEAVVLATGGYSNIFFLSTNASGSNVTATYRAWKQGAAFANPCFTQIHPTAIPPSEA
                     YQAKLTLMSESLRNDGRLWVPKQKGDSRSPEQIPEPERDYFLERRYPRFGNLAPRDLA
                     SRAVKHICDDGCGVGPGGLGVYLDLSDAISQQGLEVVQERYGNLFEMYQRITGEDAYK
                     TPMRIYPAPHYTMGGLWVDYNLMSTVPGLFVIGEANFADHGANRLGASSLMQGLADGY
                     FILPYTIGHYLATTKSGPVGTGHPDLRAAEERVTNRVARLLRGRGRQTAASYHRSLGR
                     LMWDLCGMAREEAGLKKALEAIPALREEFWHEVAVLGSGATFNQALEYAGRVADFLES
                     AELLCYDALHREESCGAHFREEHQTPLGEPLRDDANFSYVSAWEYVPNGRPVLHKEPL
                     VFEFVKPTMRTYQ"
     gene            complement(38396..39109)
                     /locus_tag="VFH05_10440"
     CDS             complement(38396..39109)
                     /locus_tag="VFH05_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HET6678680.1"
                     /translation="MKKAARFDSLPMIRFFGHILTSLGSKVLIALSGLGLAGFVVFHM
                     LGNLQVFQGQDALNAYAAFLRDMPMALWSARVALLAIVGLHIGLSVRLAIQNRRARPA
                     GYVVHHYRRASLASRTMTLTGSLLVLFIVFHLVHLTAGWLDPEFHNRVDLRGQRDVFG
                     NIVHAFQNPLLVAIYLAGQAVLGLHLSHALSSSLQTLGLEHPMLDRLFRGAGPLVALL
                     VVVGNVAIILAIALGLVHA"
     gene            complement(39157..40407)
                     /locus_tag="VFH05_10445"
     CDS             complement(39157..40407)
                     /locus_tag="VFH05_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6678681.1"
                     /translation="MDAADLVIVGGGAAGLAAAIAAAERIGGRHVVLLDGAKTLGAKI
                     LISGGGRCNVTHEAVSASDFFGNRRIIKNVLAAFPVRETIDWFHALGVELKREDTGKL
                     FPTTDKARTVLNALLNRCQALDINMFTGHRVSRIECGLDGRKGFTVVHSRGAIHTRTL
                     ILATGGRSLPKSGSDGSGDVLANDLGHSVTATVPALVPLLLDDKMFHRELSGVSQEVT
                     LMTVLAGKAVDVRTGSLLWTHFGVSGPVVMDASRVWTLARERGEHAEIYLNVLPGRTF
                     EQARHWFIMQASESPRRSVVKTLALVVPERFAQAVCAAVGCNPQRAAAQMPRSERDRL
                     LSVLTRLALPATGDRGWNVAEVTAGGVPLEEINFRTMESKVVRGLYLIGEILDCDGRI
                     GGFNFQWAWATGHLAGCAAAASVS"
     gene            complement(40513..>41289)
                     /gene="otsB"
                     /locus_tag="VFH05_10450"
     CDS             complement(40513..>41289)
                     /gene="otsB"
                     /locus_tag="VFH05_10450"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET6678682.1"
                     /translation="NHDGRSGVIHALSEAGLRALRDAASRPLLYAFDFDGTLAPISSD
                     RHAVTLTLGVAEWLRELAKRVPCAIVSGRALADVSLRTNGIVPHVIGNHGIESPLTSR
                     STLTEAERICADWKRELERTLAPMILLGAEIEDKRYSLTVHVRLAQDPADAVRTSLTW
                     LRRLSPEPRLIEGKYSINALPPGLGGKGEAALALMNHLGRTGLFYIGDEETDETVFSL
                     PDGVTMGVRVGRQAESRARFFLHEQSEVEELLRLLVQCMA"
BASE COUNT         8517 a        12729 c        12026 g         8017 t
ORIGIN      
        1 acttgagttt tcagggtctc acggtcgctg gagaacggga gggcgtgatc caagccgtag
       61 ccgaccgcat cacgccgctc atggagtaaa tgatctccgc accggagttc agtatgtcgt
      121 taggtgcgag acctcctcgg agaacccgat gggtcctttt cctgtgcgcc gtcctggtgg
      181 gatccgtcgg ctgtgccagc agtcatggtc tttcccgtga ctctcttcac gacgtgatct
      241 tgcgggaaga agccggtttt ctcgacccac cgtcggcagc ggccgctcct gctccgcgcc
      301 atgcgcccac gctcggtttc tatttcaaac cgacggcatt cgtgcatcgc gaatttgaat
      361 ggacgagccg cgaccgcgat gcggtactga cctgggctca aggactctcg ctgggcgagg
      421 gagggcggag caccggcttt ctgacaccat cctcattgaa aggtcagagc ttgcgggagc
      481 tccgggccac tgctgcgcgc tatggggtcg actgggtgtt ggtgtttgac ggggccgccg
      541 ccgtcgaccg ctataacaat tacaaggcac ccttgctgta ttggacgatc gtgggtgcgt
      601 atctggccgg cggtactcac agcgatgcgc tgtgtctcat gaaggcgacg gtgtgggacg
      661 tcaagaccgg agcccgtctc ttcgacgaac acgccgacgc aatcacccgc accgtcggcc
      721 cggcggcatt agtcgacgac ggccgggaga ttgaacgagc gaagacccga gcaatggaaa
      781 cgttgctgca gcggctcact gaccggttcg ccctgttggt cggaaggcgt tgatgccgcc
      841 gatagacgcc tgcaatgccg ttgcggtccg gacggtgttc ctgctctcgc tgattacacc
      901 ggtttgcagc atggctcaaa cgccggtcag cgaacccccg cccgtagaga agtcatccga
      961 ctggcatctg agcgcgtatg cagacctcgc ctacctgctg gacttcaatt ttccggagaa
     1021 ccatctgtgg cgcagccgca gcacgagtca gcgagtcaac gaactcgctc ccaacatggg
     1081 ctttctcaat cttacgaaag agatgacgac cgattcgcgc tgggggatgg aagtcggcat
     1141 ccaaggcggt tacgacgcaa aaacctttgg cttcctgcag gacgagccga gggtcggggg
     1201 ctcggatacc ctccggcaca tacagcgggc gaacgtgtcc tatctcgccc ctgtcggaaa
     1261 tggcttgagc atacaagccg ggctgttcaa cagtttgatt ggatatgaat cgttctatgc
     1321 caaagacaac gccaactaca cccgttcgtg gatcgcggat aacacgccct atatgatgtt
     1381 cggagtcaat gcgcgctatc ctgtgaacga ccggctgacc atcgcgggct tcatcatcaa
     1441 cggctactat cacctgtccc gtccgaatga tcagccgagc tatggcacgc aaatcgtgta
     1501 cagacccgcc gcagacgtga cggtgaccga aacgctctac gccggaccag accagagcaa
     1561 gacggccttc gagttttggc ggttctatct gaacagtatc gtggaacgga aaactgacga
     1621 cttcatcctg gccttcagct atgacatcgg aaccgaatcc attgcgaatc aaccaggtac
     1681 accgcggacg ttcgtcacgg gaggcaacgt cgtcgctcgc tggcgcatcg atggccattg
     1741 ggccgttgcc ctacgaccgg aattctattg ggatcggaac ggcagatgga ccggacacga
     1801 gcaattcctg aaggcggtga cgacgacgtt ggaatacgcg gtgccgttcg gctggacgag
     1861 gactcgagcg aggctggaat accggtggga cgaatccgtc ggggccggag gcgggtttta
     1921 caaaggcggc gatgtggcgc caggaacacc gggtctcacc gcctctcagc accttctgat
     1981 catggccttg ctttggtcct tctactcacc gtgatggaca acggcgacac acccgactac
     2041 cttgccagga cacaccacat ccggcgtgtg ccagactttt ggtccgtgac cggctcaccg
     2101 ctgtagaagc tgatctgcca ggcaacgatg tcatctttgg gatcaggcgt gctcgtccag
     2161 tagatttctg acttgatatt cgaaaaggga tggccagccg gaagggcggg atcacgttgg
     2221 gccggatcga tcagggtctt cagctcgtct acagaaggac cgcgccagcc tttctgtccg
     2281 cccacctcct tggccgcaca gcgttcgttg gagcgactcc acacgtcatg ttcccggtcg
     2341 ggttcctgct cccagatcaa ccccgtctga ttgtccttca ccgcggcgcc gtccatcaca
     2401 agagagaatc ggccatcgct tggtgcggca tgacccgcat cagaaaggga ccagagcagg
     2461 acgaacgtca tacgcgcgag cgtcagtctc acagcgaatg cactcgcttt tgcatgggtg
     2521 atctccgttc ttccggttat ggaataagag tgtgacgaat gcagcttgtc ttttcaatgg
     2581 tcagtcaggc atgctcagcg cgcttttgcc gctttagctt cgcacctgtt tcctaccaga
     2641 ccatgaagga ggcgctcatg caggacgagg aacgacggcg gaggattgat gtcctcgcca
     2701 tcggattatt tggactggct gtcggggcat tgacactcgg catcgcacag gttggggcaa
     2761 tttccgagtc gaataaggtc ggcaccttgg tcattgcgct gatgttcggc ggcgtggtcc
     2821 agatattggc cggcattaca gatattcggt atcacgaaca actcggcggc accgctctca
     2881 ccatgtatgg atttttctgg acgaccgttt gtatcgcgaa gttggtcagc gaaagcacgt
     2941 cgttccacct cgatatgaca ctctatgctc ccatcaattt cgtctatgcg gccttttcgg
     3001 ccgtcatggt ctacctcacg gcctaccgca atgtgacgct ctgcatgctt cacattgtca
     3061 ttacggtgac cttctcctcc agcgtcctgg tgcaacttaa tctgatcact gaaacagtgc
     3121 ccggcctctt ccacatgatt gtcgggttca tggccttcta ccatgccgtc gccagcctca
     3181 cgcaagcttt cacggggcgg cagctggtcc cgctgggccc tccccttctg caacaggatg
     3241 tgagcatggc acgatagctc atgccagggc atgtccagga gacctaccta gtggaatgaa
     3301 aaggggatcg gcgcaaaggt cagtacaaac accgcgaacg cgatccatcc gaccagaatt
     3361 cttcctcgtc cgagcggcgc ctgcggatca agaacgggtg ggtgggcgag gccccacatg
     3421 ccagccatga aggcccacac aaaccatccc ggccaaccga cgaatcccaa catgagcagc
     3481 aaggggagga cagcaaaggc catcgtacgc tggccctctc cccataaggc ataagcgaca
     3541 tggccgccgt cgagctgtcc gatcggaatt aaattcaacg acgtcacaaa cagaccgaac
     3601 caggctgcaa acgcgatcgg atggaggagc acgtcggcct ccggcggcaa tgcaccgatg
     3661 atcagccagg ccatgaattt cagcaacagc ggttcaccca actgcaggcc aaacgtactc
     3721 gttcgatcca cgacggtcga caggaataat ccgatgacga gcgcaaccac ggccaccacg
     3781 aaccccgcca gcgggccggc gacgccgata tcaaagagcg cacgccggtt caaaatcggg
     3841 cctcgcatgc gaataattgc gccgaacgtg ccgatgaagt gaggcggtcc ggggatgaac
     3901 agcggcaggg aggccggtac tcgatggatt cttgacaaga tgaagtgacc gaactcgtgc
     3961 gtcaccagga taaagagcaa cgttccggcg aatggaatgc ccttccagag catctccgga
     4021 tgttcccaga gaaagaccag cggtccgcga atcgtgccgc tgtaggcttg ataggcacct
     4081 gcccagagcg tggtgaatac ggtcaacaga aacaggagga ggggtaacgt ccaccgagaa
     4141 aaaggcgatt cctcttcggc gcccgacatc tcagcttcct gttcgacagg cgtccgaacg
     4201 gcgcccgctg gcccggcggt cttcaggttg cccagtgggt gatccatgcg gtccagccta
     4261 gctcacataa aaagggttat gatccagttc ttcccgcact gtcgtgccag ggccatgtcc
     4321 cggcaacagc aaacaatcgg caggcaacgt taacacgtcg cggcgaaccg actcgaggtg
     4381 ggtcgcatac agccccggtg gattggagcg gccgatggag ccggcgaaca gggtatcgcc
     4441 cacgaacgcc accggacccc atggcatctc gaggcgatag cagacgccgc ccggcgtatg
     4501 ccccggggtg gtcaggcacg tcatagccaa ccgtcctacc tgaatgatcc ggccatcgtg
     4561 cggcgtggct cgctgttgct ctgaaggctt ccagtgaaga agcgtcacat cctctgttcc
     4621 gagatagatg ggtacgggcc aacgactcac aatctgatgt atcccctccg catgatccgc
     4681 gtggccgtgg gtgagacaaa tgcctaccaa gcgcagacgg cgggcctcga ggatttccag
     4741 catcatgtcg gcattgtatg ccgtgtcgac gagcaccgcc tcgccgctat catgcacgat
     4801 gtagcctttc actccgtagc cgctgatttc tcccagcacg gtttcgatgc ccggcatcaa
     4861 gggcagggtc ttgggaaccc acttttcaag cgcgatctgt tcaagcgctg cgagacgcaa
     4921 cccgagggcg gtcgaaatgg ctcgaagctc agtccgatcg cggagttgat cgccccgttc
     4981 gagggccgtg atatccgctc ccggcaagcc ggtgagacgt gccacatccc cgacggacag
     5041 cttcaatccc gttctagcct tcttcataat gtcacagaga tcgtcttcaa gcggcatcgt
     5101 acgccttcca gcctctcacc cttctggaat caacttaatt tctttgacct cgccgaaact
     5161 ggacagaagt ttcggcaacg cttcgccggg gccgactgcc acaatcgtca ggcggtccgg
     5221 atggaaatgc ttttgagccg cagccagcac gtcttctctg gtcaacgcca ccactcgggc
     5281 gcgtaattgc tgcaagaaat ccttgggcag accgtcgtac tccagttcga caaagcgccc
     5341 gacaatggcc gagggactcg cgaatgaaaa cacgaatgaa ttgacgtacg cctctttggc
     5401 ttccgccagt tcttcattgg tgactaactc cgtgcgcatt cgttccatgt tggcgacgaa
     5461 ccggctgatg acctcctggg tggaaggcaa cttcgtttca gcccgcatca gccacacccc
     5521 ttggtcatgc atgccgacgt tcaaccggct gcccactgaa taggcgaggc cgcgtttcgt
     5581 gcgaacgtcg ttgaagaggc ggctccggaa cgagctgcct ccgagaatgt cgttggcaat
     5641 ggccaaggcg acataatcgg ggtcatgctc tttaatgctc agatggccga tccgcaaatg
     5701 cgtctgggag gtatccttgt tcacgaaccg gacgatcgaa ggcccgttgc tttcacgcgc
     5761 atcggcgatg ctcaagtccg gcacatctcc cttcttccag tcgccgaacg tctggcgtag
     5821 cagctcgagc atctcggcct gccggaaatc gcctgtcacg ccaagaatga tgccgttcgg
     5881 ccgaaccgtc cgttggtgga atgcgatcaa atcatcgcgg gtaatccgct tgaccgagtc
     5941 gatcgtgctt tccctggcgc tgggatggtc cggcccatag agttgtttga gaaattctcg
     6001 gccgacgatc gagcctgggc tgtcctggcg gcggcgaatg ccctcgatca tttgcagctt
     6061 ggccagttcc acgcgtcccg cttcgaacgc cggctgacgg agcaggctgg caaatatccg
     6121 gagcccccgt cctaactcct ttcggaggac gtcgagcgaa gctgaaccgg actgtcgact
     6181 catcgagatt ccaatgtcga tggcatactg ttcgagttct tcgtcaattt gctcggccgt
     6241 gaagctcccg ccgcctccgc ttcgcatgac ggatccggtt aaggctgcca gtccaatctt
     6301 gtcctgtggt tcaagccacc caccggtccg catcgttgcc gtaacgttca cgagaggcaa
     6361 ttcatggtct tcaagcaaat acaccaccat gccgttctcc agcacgacgc gcgcaggctc
     6421 tggtggcgag aattcgaccg gtgtgaaatt catcgttcgt ggatcgcgcg tcacacctgc
     6481 agcaaaggtc gtcgccgctg caaccaatcc caagacgagc actgcaagct tcgcccctgt
     6541 ggtgacccat gtgcgccttg tcatcatcgc gcccccttgt ccgacgtcat ggtcgccacc
     6601 tgcttcgacg cggtcttttt cacgagcgtg gccacagtgc gattggactt cgtgaagtac
     6661 tgggttgcga cacgctgaat gtcggcgggc gtcacggcgg cgatcttgtc tcgcgacttg
     6721 aggataaagc gccagtctcc ggcgacggtc tgatagaggg ccagttggga cgccagcccg
     6781 ctattagatc gcagtgtccg aaccatgtcg gcatccagat tgttcacgat tcgctcaagc
     6841 tccttagggg cgaccggttc agttttaagc cgctcaagct cggccaagat ggccgtctcg
     6901 acttctgcgg tggaatgagg cgccagcggc gtcgcactga gcacaaacaa attggccgag
     6961 cgaacaccgg gatagttcgc gtccgaattc accgaagcgg ccaggcgaga atcgcgtaca
     7021 agcttgctat aaagccgcga tgtcggtccg tccgttaata actcgtctat gacgtcgaac
     7081 acataatcgt cgggatggcc caaggccggc ttatggaagc cgatggccaa cgacggctcg
     7141 gcgtcgaact caacttctac acgacgctcg cctcgttgtg gcggctccac ggtgacgatc
     7201 tgaggcgggg gcggtgcggc cgggaccttg ccaaacgtat tttcgattaa ggtcatcacc
     7261 tccttggcgt cgatatcccc gacgatgccg atcgtcgctc ggccgggacc atagtacgtc
     7321 ttgaagaagg cttccgtgac ggcgggggtc aatgacaaga tgtccgactc ccagccgatg
     7381 gttggcaccc cgtacggatg ggcgcggaag gcagccgacg tgaaggtctc atacaggagg
     7441 ccgctggggc tgtcgtcgtt ccgcaaacgg cgctcttcca tcacgactcc ccgttctttg
     7501 tagaactcac ggaggaccgg actcgccatg cgatccgatt cgattgcggc ccaaagtggg
     7561 agccgattgg ccggcagact gatgacatag cgggtgagat ccttcccggt cgatgcattg
     7621 aggccgacgc cgccgtggcg ttgatacaag agcgccatct cattgccggc cacgtactgg
     7681 gcggcctggt cttgcagctc cttgaatctt ttttgcagac tttcaatggt gcgcgtctcg
     7741 gcagggtcaa ccggcgtgtc ggcagttcgc ctggccagct cccgctgtcg ctgctcgagc
     7801 tcagttccca cttggtgcaa ttcgtcgagg atcggttttt ccttctcgta gttttttgtc
     7861 ccgaccgtcc tcgtgccttt gaatgccata tgctcgtata agtgggcaag gccggtttgg
     7921 ccgacctgtt cgttgacccc gcccacaccg aaggtcatgt tgatcgatac gacgggcgct
     7981 tgatgccgtt cgaccagcag tacggtcagg ccgttcgcca gcttgtgctc aatgactcga
     8041 tcggccaggc cggaagaacc ggcagcactc aagctcgcca gaccgagcac gaccgccaca
     8101 cagcacactt gaagccagcg tgacactcgc cccatcggtt gcgccagcct cgctctattc
     8161 gtcctcgctc tattcattcg aatatctcca tcaattcttc cggtcgttca atgaaccaat
     8221 ccggctgaca ggcggacatc ctttcccgat tacccattcc gtagcccacc gcacacaccc
     8281 gaatgccggc attgtgtccg ccgttgatgt cgtttgtact gtctccgacc agcaccgtcc
     8341 gctctggcac ggtgttgagc cgttccataa tatgcaacaa catgcctggc tccggcttca
     8401 atccaaatcc attgtcgccg ccgacgacga agttgaaata ctgcggtccc agtccttgca
     8461 ggatcacatt ggtgtattcg atcgacttgt tcgtggcgac tgccttgcgc ttgtcgccaa
     8521 aatatccaag cactttttcc actcccgggt aatacaccgt ccgatcgagg cagtgggcga
     8581 ggtagtgccg gcgaaacacc cccagcgcct cttcgaagct cacgcgattg gtctctccga
     8641 cggccagccg aagcaatttc ttgacgccgt cgccaacaaa cccaaaaatt tcttcaagcg
     8701 gccgttgcgg aagccccaat tcttcgagcg taaagttgac cgaggccgca atgtcccatt
     8761 tggactcgat gagcgtgccg tccaggtcga aaatcaacaa gtcgatcggc gcctttccca
     8821 ttgcctttcc tacttgacct ttcgcaattc gtcctggagt acggccaggc tctcgcgctt
     8881 ggccggttcc gtttccttca cccatgcttc ccgcacgtaa ttgatcatcc gcttctggcc
     8941 gacatgcggg ggcagcacgg gttcaataat gcgccagcgg tttcgtatcg atttgactcg
     9001 gacgcgaatt tcttccacgg tgtcgtccgg gagaaacccc gccgtctcga ggtagacaga
     9061 gccaataact tgaaacgtga ccgggatcac cacctccaac gggttcacga tttcccaatg
     9121 ccgatgatgc tcagggacgg aaaatccccg gatgacgatc accttgcccc aggccggctc
     9181 agcctgccag cttcgatagg gcgccaacgc ctcgaacgac atcgaatcga gcttagcgcc
     9241 cttttgatca agctcaaaat acttcttcac tatttcggcg ggagaatggc tcaagccccc
     9301 cgtttgagcc aatgcggccc cgttgaacca tgcgagcaca ctcagcatcc acatgatcgc
     9361 catgatggat cgaccagcga tccctatggg cttgcacagc agcattgtca gcgtgctcgg
     9421 acggttgaga tggtttcttc ggttatcgcg ggaaaacatt cacaccggcg acgacattct
     9481 accaaaggcg ttcttcgaaa gcatgtttcg acgggaccgc gaagaggacg gcgttgacgt
     9541 atctcgagcc tgcgtgctag cgttttcaca gccgcaactc tcttccgcaa gcccttgatg
     9601 agatgccgag tcccatcacg tactgggtgc ttctgctcgc tgtctgcgcg gcggcgccgt
     9661 gcggaagtcc ttccgggagc cgcgcccacg cactcgaatt ggcaaccgaa gtcaacgagg
     9721 ccgtggtcat gccggaccgg ccctcgtgtg ttccgcgatc ggtcatcgag cgcacggcac
     9781 ccccaaaagc ctatgacgtg ctggcagcgc tccaacaacg gagtggagtc cccctccccg
     9841 gttacatcgg aggaaacgaa ttcaggaatc gggaacgccg gctgccgcat ggccgctatc
     9901 gggaatatga cgtcaatcgg aaaataccgg gccgatcgcg cggcgcggaa cgcctcgtca
     9961 tcgagcagcg cagcggtaag gcctactata ctggtgacca ttaccgaacg ttcattccat
    10021 tgaattagac gacatggctt cgcacacatc gccgctgtct ccgacgcgcc atttgcaatc
    10081 gacgaaacct ccctggacca tcctggtggt tctgaagacc ggacagcgcg ccgaggccgc
    10141 aattacgatt ccggctggat acgttctgcg tgtcgtgcgg ggcgccaaat gtcggtacac
    10201 cgcggggctc ttttctgagt tcgccactgc gatggatttc cccgactact tcggccacaa
    10261 ctgggatgcc ctcgaagaat gtttgaccga ccttgaatgg ctgccgggca aggggtatgt
    10321 gctactcgcc acgggggcgg aacagatcct gaccgccgac gaggaggaat tcgccacgtt
    10381 cctcgaagtg ctgagcgatg ccggagaggc ctgggcatcg gggcaggcgg gtgcggaggg
    10441 taaaccggct aaaccttttc acatggtgct ggccgtgtcc gagcgcgacc aatccaagct
    10501 ggcgcattgg ggtattccgc aagtcacgga aataccttcc ccgcgatcca gcaggaaacg
    10561 ccggcggtaa caagacagaa cggccggctt acgcccgttt gttttttgtg aaatttaaga
    10621 gcccggacca gcgaaggagc gtgaaggtcg tggccgctct cacgaagctg ctcgtgccgg
    10681 tataccgggt tgaggcctgc cagtgctcgc ggatctgatc tcgatgcagc agacaaatgc
    10741 cgtgcgtctc acgcgcgtca tcgaaggggg ccttttctcc gacgaactcg tgcttgcctt
    10801 ccttccgaca ccatgaacac acaatcttca ttcgaaatcc ttaagctgtg aataccgttt
    10861 gaaaacatgt acgcatctcg actctacgca atagtcaggc cgcgaagcag agacaggagg
    10921 aactctacga attgccgtat aaccgacccc ttctattgct tcaacacccc cctcaactgt
    10981 atcaaaatgg tgatgggcga cgtatccatt tatacacttt ttgacctcac ctgaggcaat
    11041 tctcggcacc taactgcgca gcagcgctcg cttgaccgtc caacattgac atgttacggt
    11101 tcgcggcctt tatgaaaatc acacgttcta tcaagttgtt tgagaaagcc cagcacctca
    11161 ttcccggagg tgtcaacagt cctgtccggg ccttccgatc cgtcggaggt caaccacggt
    11221 tcatcaagcg ggccaaaggc gcgagacttt acgatttgga tggaaatacc tacatcgatt
    11281 atgtgctctc ctggggaccg atgattctcg gtcacgcctc accgaccgtc gtgtccgcga
    11341 ttaagaacgc tgctgaacgg ggcacaagtt acggagcgcc gaccgaactc gaagtcgaac
    11401 tggctgatca gatccgtggt gccttcccct cgatggaaaa gagtcggctc gtcagctcgg
    11461 gaacggaagc ggtgatgagc gcgatcagag tggcacgtgg cttcaccaaa cgcgacggcg
    11521 tcttgaaatt tgaaggttgc taccacggtc acagcgacta tctgcttgcg aaggccggct
    11581 ccgggctgac gacgctcggc attccagatt cactgggcgt cccggccgat tttgcgaaac
    11641 atacgttgac ggctccgtac aacgacattc gtaccgttca acggattctc cgggaccgtg
    11701 gaaaggacct ggcctgtatc attgtcgaac ctgtagccgg aaatatgggc gtcgtccctc
    11761 ccgcacctga ttttctgccg gcgcttcgcc aattggcgac cgaccatgac atcctgctga
    11821 ttttcgacga agtcatttcc ggctttcgcg tgcactatgg cggggcgcaa gcactctatg
    11881 gcgtcacacc cgacttgacc gtgctcggga aaatcatcgg cggcgggctc ccggtggggg
    11941 cctatggcgg acgaagagac attatggatc tcatagcgcc ggctggtccg gtctaccaag
    12001 cgggaacgct atcggggaat cctctggcgg tgacagcagg actcgcgacc ttgaagcaat
    12061 tgcgggccaa gaatctgtac aagcagcttg aggaacggtc ggccgccttg gcacgaggcc
    12121 tagcggatga agccaaacgc gctggtattg cgctgaccca gacgagagtc ggatcgatgc
    12181 tgacgtcgtt ttttacgcag gaaccagtcg ttgactggga cagcgccaag aaagccgaca
    12241 cgaaacggta cggattgttt tttcatggga tgctcgagca aggcatttat ttggcccctt
    12301 cgcaattcga agccgccttt ctctccacag cgcacacgtc agccgatatc gagaagacca
    12361 tccgcgccgc tcgcgtagcc ttcaagagtt tatagttcac gaaagagctg tattaggtca
    12421 gctacagggt gagctgattt gatacactat cggcggtcta ttcgtcgtcc tcgtcgtctg
    12481 cttccaacgc ctcctgcctc ttctgctctt ccatggcatt gtgcaatagc atgcgaatga
    12541 acatcgaata cagcgagccc ttctccagtg tgccgccagg aggaatggcg attttccctt
    12601 ccttgatcat cttatccatc cacaccttct gatctggcgc gatcagaatc gggatctcga
    12661 ctagaccgac ccggccgaac atttccatcg tcgcctcctc agcatgaagt gtcgttcgtc
    12721 tctcgtgtcc ggcgaaaatg aatgtcccct taggagattc gtcctccgag cgacgagata
    12781 tgtgggtatt gcagcatgcg gttttcttgg atgtcaactt cgcggcgccc gtaatggcac
    12841 gaagcctgct tcagacgtaa ccatgccacc acgccgcgta ttggatgtga tacctatcgc
    12901 agtcctcgcg atcctcggtt taattgaaag tgctcattca caaccatcca ccgcgcctcc
    12961 atcctcgttg ccgaccacgc cgttgacgcc aaacatgaac aatccggctg gtccggcgac
    13021 ctgcgagctg tgccgagccc ttgaagggcg agcaggaagc gatcttcccc gccatcgacg
    13081 tggacgacag aaggacttac gccctcacaa gcaggcgccg gggatcacca catcgcccaa
    13141 gcgaagtctc atgagccaac tgccccacca caagctggaa gcgaatcatg atcacgaaga
    13201 ccgatgaagg caggagcgga ccggtcactt cttgtgcttg ttgtgcttct ttgttggtcc
    13261 ctcggaaaac agcaaccacc ccaacacgat gaagccaacg gcgacgcccg cgatggccaa
    13321 ccacgtcccg atcttatcca tgattgaact gtagcgcgac gaccgtctcc ttgtcgaggc
    13381 gcctgacttt tccgttgccg tcggtgacgg cgagccgcgc ggacccctcg acgatcacac
    13441 gtccgctctg ccgctcgcgc acgacgtggg agaaggtaat cgaggccgga ctcatctcgg
    13501 gaatcatggt ggcaatgtcc agaatatcgc cgtaacgggc tggtgctcga tagtccacct
    13561 ctgcatgcac gaccacaaac aggcacccct gcttcatcaa tgcggcgacc gagagcccgc
    13621 gcgcttccag gtagtcggtc ctcgcccgct cgaaatattt gagatagttc gcgtagtaga
    13681 cgaccccgcc gcaatcggtg tcttcgtaat agatcttgat ttccatcgaa tcgtcgttcg
    13741 tatctctgaa gcgtcactcg agaaagacgc ctgatgagag cttcatcagc tgacactcat
    13801 gaactgggag taggttccca tgacgttccg acatgaaaac gtcgcttcac tacacagtga
    13861 gcgtggggaa ttttggcaag gaattttcgt cggcatgcgt gaccttcatg acgacctcga
    13921 acagttgctg gacccgttcc gctttgaccg gttcgccccc atggcccagc acggcacggt
    13981 tcgcggcatt gaacaacggt ttcatcgtcg gccactgccg cagaaacgat tcgcccatct
    14041 ggtcccccag tccggccaag acacggaatt gcgcctggag ggacagcaca tatttgccgt
    14101 cgatatcgtc cgaataacag gagcggcaga cgtcctgcat cggttgaggg agctgctccg
    14161 gttgcacgtc ccaggtcttg atccggtact gtttgaacag ctggcgctga gaaaaggctt
    14221 cgagtgcccg aaccaacgac tgcatggctg cctctgcatc gttggccacg cgcagtttcc
    14281 gcccggcatg cgccagcaag tcgaacgcga gtaactcttt cacttccgcc gggtcgagca
    14341 cgagcttttc caggaaaccg gcgttggcct tgaccgccgg gaggctcgcg ttcaagccgg
    14401 gcggcccgcc gaacaaggac gccatttcaa tcgccttggt cgccactttt aacttttccc
    14461 aggcctgacg atgctgaaag cggtcccaga gatcgtagcc atcggccagg tcggccagtg
    14521 cacggtacag cggcttctgg cctccgctga cacggctttc gatccgccgg aagatggcac
    14581 cggccgccac gaacgcgccg cggttgaaga aatcgaatgc ctctcgtctc gcgacttctg
    14641 cgccctcatc ccaagggttg accgaaatcc agaggcgctc accgccttcg agtgccgcag
    14701 ggtcgccttc acggccttcc cgttgttcaa ccaacgaggc aatgcgcgac gtccatggca
    14761 tcgtgaccag ggtcagcgcg ccggccatgg ctgcggtcgc gccgctcatg tcgacgacga
    14821 gcgcacccag tccgatttcc cacgcccgaa gcatgggggg cagagagcgg gcgagggtcc
    14881 gataacacga aggaaagtga tccacatccg acaacggcac ccaatcccac cgtcgtggca
    14941 tgtgactgat gtgcggttgg acatctgttt ccaccaacgc tttcgcggac tccggcaaga
    15001 cgaagcagac gcattccgga cgcaggcgat tgagcgaata cacgaccgcc ggaagatcgt
    15061 ccttcagcgc gacgacaagg gcagcgactg gttggtcttg agccatttcg agcggactat
    15121 aacacgagca tcggtcgaat tcccggctcg tagtcacgtg cttgacggtc ttctggaagg
    15181 cgcttagact ccccgccagc gttttcatca gcacccaatg aggtttctcg atggtctggt
    15241 ggtattggat ggttctcggc ttagcgttgc tgggggcgga aatgacgacg cccggcggat
    15301 tttacatttt gtttttcggc ctctctgctc tggttgtcgg aaccctggcc ggcctcgaca
    15361 tggttgaggc ggcttggctc cagtggttgt tgttctctat gattgccgtc ggctccttac
    15421 tgatctttcg cggtccgttg ctttctcgac tccgcgacaa tcgccgcggg gacgcggaca
    15481 tcgactccat ggtcggcgag gtggcgatcc cgcttgaaac gctggcggtc ggcgccacag
    15541 gcaaggccga actgcgcggg acgacatgga gcgcaaaaaa tgtcggcacg acaagcttca
    15601 gcaaaggtca gcggggaaaa gtgacgaaag ttgaaggtct gacgctgtgg atcgtggccg
    15661 aatgagtgaa tggagcgcgg cacgtcggcg cgtgcaacag gaattctgat ggaggaggtc
    15721 agcatgtcag caggcacgtg ggtggtcctc gttctcgcgc tgctcgttct ctacgtgatc
    15781 gcgaagaccg ccgtcgtcgt gccgcaacaa agcgcctatg tggtcgagcg gctggggaag
    15841 tattcggaca cgctgaatgc cggctttcac atcctcttac cgtttttgga ctccattcga
    15901 taccggcatt cgctgaagga aaccgccgtc gatatccccg aacaagtctg catcactcgc
    15961 gacaacgtcc aagtgtccgt ggatggcgtc ctctatctca aagtgttgaa tcccgagcgg
    16021 gcctcgtacg gcatcagcga ctatcatttc gccttgtccc agcttgcgca gacgacgctc
    16081 cgcagcgaga tcggaaagat cgaattagac cgcacctttg aggaacgcac caacatcaac
    16141 atccaggtcg tcaatgaatt ggacaaggca tcggagccct ggggtgtcaa ggtcttgcgc
    16201 tacgaaatca agaacattac gcccccaaag gacgtgctca atgcgatgga aaagcagatg
    16261 cgagccgagc gcgaaaagcg tgcgctcatc ctcacgtcgg aaggcgaacg ggacgccgcc
    16321 atcaaccagg cggaaggcga aaaacaacaa gtgatcaagg cgtcggaggc caagaagcag
    16381 cagcaaatca acgaggcgga aggcgccgca gcggccatcc tggcgattgc gcaggccacc
    16441 gccgaaggat tgcgcaaggt cgccgatacc attcaacttc ccgggggcca ggaagcggtg
    16501 cagcttcgtg tcgcggaaca atacatcacc aagttcggcg aactggccaa gacgaacaac
    16561 acgctgatcc ttccggccag cgtctcggac gtgggttcga tgattgccgt ggccatgtct
    16621 gccattcggc agacgacgcc gtcagcttcg ggaaaatcgt aagttccgtg ccggatcttc
    16681 atgagtccat ttcccatctc aatccggatc ctgcgtgttt gacaggatgg gacccctcgc
    16741 ctagaattgc acacgatgaa agacttggtc gtacaagcct tcaccgagag cgccgccgtc
    16801 aagcagcaat tcgtgcgcga gcacgcggac cgcatcgtcg aagtggcaaa gctgatcgtg
    16861 aggacgttcc gtgaggggca taaagtgttg ctgtttggga acggaggcag cgcgacagat
    16921 gccgcccaca tcgcggcgga attcgtcgga cggtataaac gcgagcgggc gccgttgcct
    16981 gccatcgcgc tcgccacgga cgtggccgcg atcacctgca tcgccaatga ttatggatac
    17041 gacgagctct tcgcgcggca agtgcgggct cacggtcaaa aaggcgatct cgccatcgcg
    17101 atcagtacca gtggaaattc cgtcaatgtg ttgaagggcg tcgaggcggc aaaggcgtgc
    17161 gggatgacga ccgtagcgtg gaccggcgga accgggggga agttagccgg cttggtggac
    17221 tatccctttg tcgtaccgtc gaaggtgaca gcgcggatcc aagaaagcca catcaccctg
    17281 gggcacgtgc tgtgcgaatt gactgaggaa gagctccttg gcaaagcctc ctgaccggag
    17341 agggcactcc cccagccccc gccgcttgat ctctccgatc aatgtctctg atttaaaaac
    17401 ctatccgctc aagaagcgtc acagcaaagt gcgcgtctcc gattttgccc agccctggcg
    17461 caaaggaggc agcttcagtc ggttctatcg agcgcttccc gatattcttg ccgtcaaaac
    17521 gctccgcgct gtggcccgtg ccattgccca ggctcaccgc agaggccggc cggtgattgt
    17581 cgggatcggc gcccacgtca tcaaggtcgg tctcactccg attctcgtca cctggatgga
    17641 gcaggggctc gtcaccgccg tcgccatgaa cggcgccggc atcattcacg attttgagct
    17701 ggcgctcatg ggccatacct cggaagaggt cgatgacgag attgacgacg gacggttcgg
    17761 tatgaccgaa gagaccggac gcatgctcaa tgaagccatc acgcaagggc accggaacgg
    17821 ccagggaatg ggtgaagccg tcggtcatta catcacccgt tcgaaaaacc agtttcccaa
    17881 ccggcgcacc agcatccttg ccatgggcgc aacgctcggc attccagtga cggtccatgt
    17941 cgcggtcggc accgacgtga ttcatatgca tccggcggcg gacggggcag cgatcggcgc
    18001 gacctccctt ctggacttcc ggcggctcgc ggcggtggtg gccggtatgg aaggcggggt
    18061 ctatctcaat atcggttcgg cggtcgtcct gcccgaggtg tttctgaaaa ccgtgtcgct
    18121 cggacggaat ctcggccata ccttatcgaa catcaccacc gtgaacatgg actttctgtc
    18181 tcattatcgc ccgctcacca atgtcgtccg ccggccgact caaaaaggcg gcaagggata
    18241 ttcgttgatc ggccatcatg aaatcatgtt accgttactc gttgcggcgg tcgcagaaga
    18301 gctggggtaa ctcctgatgc cgggtcatgc cgttccgctc agcgtctcac gcatcacggc
    18361 aatctggcgc gacctgaaca ctcgatactt tgatggtgcg cttccgccga ttgcggtcgt
    18421 gtggagcagg cggctgaccg cttccgtcgg catgttcgtc agtcgggccg gtccacgctc
    18481 ggcatggcgt gcgaacagat ccgccaacag ccgcgagatt cgcctctccc ttcccttgtt
    18541 gacacccttg ctcacagaaa cgccgtacgg cacacaggaa ctcatcagca cgctcgcgca
    18601 tgaaatgatc caccagtggc aattcgacgt gctcaagcgg cggccgaatc acgggcccga
    18661 ctttctgaga aagatgacgg agatgaaccg cgacggtgct cttgccgtga ccatctacca
    18721 ctcgctcaag caggaagtgc tggcgctgtc ccggtttgcc tggcgttgcg gacgctgtgg
    18781 gcgcatctac cgccgtcagc ggcggacgat ccagccgcgc cgtcaccatt gtggcagctg
    18841 ccgcggcgcg ctgcatgagt tgtcctcatg ggaggtcgcc gggcgttcat ccatggaaac
    18901 tgctcgattg accgctccct gcacgtcgct acaacctgcc cggcagcttg agctggattt
    18961 ttcctccgcg agcctctagg ctctctttgc actgactgct cactggacgg tgttcgatgc
    19021 tgcgatggtc gcgccagatt ctgtttggtg acgttgacgc gatgtttgcc tccgccgccg
    19081 ccgtcaaaga cccgaccctg actgggaagc cgatcgccgt cggaggcccg ccgccccgag
    19141 gcatcatcgc cgccgcaagc tatgccgtgc gggccttcgg tgtccggtcg gccatgccga
    19201 cggcggaggc cttgcgacga tgccctcacc tcctgctgat cccgccagac cgggcgctgt
    19261 atcgtcgtct gcatgacgac atgcgcaggg tcacggaccg gttgtttccg gtgaccgaat
    19321 ggggcagtat cgatgaattc tacgccgaca ctacggacct ccaaacccgg catccggatc
    19381 cccgactcct cgcgcaactg gtgaaggact cgattttcga ggccaccggc ctgcgatgca
    19441 ccatcgccgt ggcgacggga aaaaccgtgg ccaagattgc cgccgatgcg tcgaagcccg
    19501 acgggcttgc catcgtcgaa ccgggccggg aagcggcgtt tctgctgcca cggccgctgc
    19561 gggcccttcc tggaatcggg ccaaagaccg ccgcgcgcct cgagcccttc agcatcaaaa
    19621 cgatcggcga tctcttggac ccgcggtggg atcaggtcct cgcgagaatg tttggccacc
    19681 gtctgttgtg gattcgagag ctggcgcagg gccgcgatcg cgaaccggtc attggcgacc
    19741 gggaatccaa gagctcgagc cacgagacca cgttcgagcg ggataccgcc gatccggcat
    19801 ttctcgaaga cgtgttgaga ggatttttgc gcatgctggc gcgagacctg cgccaggagg
    19861 gactggcggc ggaaggatgc accgtcaaat tgaaggatgc ccgctttgcc gtcacgacca
    19921 ggcaacgacg gttctcccat ccgctcaatt atgatcccga aatgtggccg acggtccgac
    19981 aggccttgca tgaactcatg aagccgcacg cacagtatcg cctggccggc ctcacactct
    20041 tttcactcgt tccgattccc gcgggcctgt acgaccagcg ccggcgcaag gcggtccaag
    20101 ccatggacac catcatcgcc aggcatggtg cagtgatcgg gttgggcggc gtatgtgacg
    20161 atgacaacga gtagtccgcc gggcgtcttt ctatctttca atactgtcgg tatgatgaag
    20221 cgatgccggg caaagtcgtg accagtgcgg aattggttcc ttttctgaaa cgggcgcgtg
    20281 cccaaaagca gcgcgtcgtc ttcaccaacg gctgcttcga cctcatgcac gtcggccata
    20341 cgcggtatct gcaggcggcc aaggcgcttg gcgacatcct cgtggtcggg gtcaacagcg
    20401 atgcgtcggt ccggagcctg aagaaaggcg ccggtcgccc gatcgtcggt gaagcccagc
    20461 gggccgaagt ggtcgcggcg ttaggggcgg tggactatgt cgtcatcttt cccgagcccg
    20521 atcctctgac cttgatcacg gcgctcgagc cggatgtgtt ggtcaagggc ggcgattgga
    20581 cgctcgatcg catcgtcggg cgcgaagtcg tcgaacaacg aggcggaacg gtcaagacca
    20641 ttccactggt gcccggcatg tcgacgacgg cactcatcca gcgaatccgg tctcaaccac
    20701 actgagtcct gtgtcacacg ccctgtcgag ctcaaccacg ctcccctgtt gcgcgccgat
    20761 tgccgctgcc ctcgccagga agggcgcacg gccctgcctg accggcctgc atggttccac
    20821 cgcagggttc gccctcacgc aactcatgca ccctcgagcc ggggtgctcg gctccgaacc
    20881 ctggctgatc gtgaccaaaa cggacgaagc cgccgagcga ctgttcgagg acctccgctt
    20941 ctatcacggt ctccagggag tgcccgtcac ggctctcgcc ctctttcctc ggtgggaaac
    21001 cctcccttac gaatcgacgg cccctcacgt tgggctgatc gcccgccgga tgaacgccct
    21061 gcaccagatt cgatcgatgc ctgccgccaa ggtggtgacc tccatcgagg ccgtcatgca
    21121 gcggctcatg ccggtggaca cgttccagca cacagtgctg cagttcaagg tgggagccgg
    21181 cattgaacgc gaagtgttga cggctggcct tctccggctc ggctatcgcc gggtgtctgt
    21241 cgttgaaatc ccgggtgaat tcagcattcg cggcgggatc gtggacatct tttcgacggc
    21301 atatgcggaa ccattgcgcg tcgagttcct gggcgaaaca gtggaatcgc tccgactgtt
    21361 cgatcctgct acacagaaat ccacggccaa aatggaccgg gccatggtct tgcccgcccg
    21421 tgaatatctg aaaccggaca ccgccgccga tgcgctcgcg ccaatcgccg ctgacgccga
    21481 atggcgcgct cccgatctct atggccagat gcacagtctg ctcgactact tcccgacgca
    21541 accgatcctc gcgttggacc agcctgcagc cttgaagcag tcctgtgcgg acctctggga
    21601 gcggatcgac gacgggtatc tgcggcacag cgacaaagag gacgtgtcct atccctcacc
    21661 cgaacggctg ttcctgacgt ggcaggatat catggaccgc acggctgaat gcacccaact
    21721 ggcgcttgaa ccgctggctg tagccgaggg ctcgtgggaa ccggtccagg cattccccgg
    21781 ccaggtaccg gccagcgccg gcttgggtgc gcgtggcacg ccgttcagcc agaccctgtc
    21841 gatccttgag cacctgcggg aggcctgccg ggtcttgctc gtggcgagaa gccgcggcca
    21901 ggtggatcgc ctgctggcac ttctccgcga gcatgatgtg ccggcgattg aatggtcgac
    21961 gtcaagctgg tctacctcga cagacacgcg cgcgccgttt tttgtcctca acggcgatct
    22021 ttccgcgggc tttctgtcgt cggaactccg gctggcgatc ctcacggagg aggagctttt
    22081 tgcaaagggc gcccgtcaca agccgcaagc caagagcaag gccgcaacgt tcctgtcgtc
    22141 cctggaagat ctcaatgtcg gcgattacgt cgtgcacgtc cagcacggca tcgccaaata
    22201 tcgcgggctc aagcgtctgt cagtccagga cttcgacagc gattacctga ttctggagtt
    22261 ttacggcagc gacacgctct atgtcccgct ggatcggctt aatcagatcc agcgttatag
    22321 cggcgccgag ggacacgtcc cccgccttga ccggctcggt ggcacgagtt gggccaaaac
    22381 gacggcgcgc gtgaagaaag acatagaaga gatggcacag gaactggtcg atctctatgc
    22441 caaccgcgag ctggccaaac ggcactccta tgggcacgat agcaccttgt atcacgagtt
    22501 cgaagccgcc tttgaatacg aagaaacgcc cgatcaattg aaagcgatcg aggatatcac
    22561 gcgcgacatg gaagcgagca aacccatgga ccgtctggtg tgcggtgacg tgggttacgg
    22621 caagactgag gtggccatgc gggccgcatt caaagcggtg gaagacaatc gccaggtggc
    22681 cgtgctggtc cccaccacgc tcctggcgca tcagcattac gataattttt ccgaacggtt
    22741 tgcgccgttt ccgacccgcg tggcgctcct ttcacgcttt cagtcgccga aagaagcgaa
    22801 aacgatcctc aaagaggtgg aggcgggaat catcgacgtc gtgatcggga cccatcgcct
    22861 cctgcaaaag gatgtgcgtt tccgggatct cggtctggtg atcatcgacg aggaacagtg
    22921 gttcggggtc aagcacaagg agcggctgaa gcagctgcgc gcgcaggtcg acgtcctcac
    22981 gctcaccgcg acgccgattc cccgcacctt gcaaatggcc atggcgagcg tgcgggatct
    23041 ctcgatcatc gacactcccc cggccggtcg cctgtccatt cgcacacagg tcgtccgctc
    23101 cagcgacaaa ctgattcgag aagccattct ccgggagctc ggacgaggcg gtcagatcta
    23161 ctttgtccat aaccgcgtgg agacgatgga acggaccggt gcgtggctgc agcagatcgt
    23221 tcccgaagcg cgcgtggtca tggcgcatgg acagatggat gccaaaccgc tggaagccgt
    23281 gatgctcaaa ttcttccgcc gcgaggcaga cgtcctggtg gcctccgcca tcattcaatc
    23341 aggcatcgat gtgccccacg ctaacaccat catcgtcaat cgagccgaca cgttcggtct
    23401 cgcccaactg tatcaactgc gcggtcgcgt gggccgcagc ggcgaccagg cctatgccta
    23461 ctttctcgtc cccgacgaag ggaggctgac ggacgatgcg caaaagcgct tgacggccat
    23521 ccaacagttc accgagctgg gttcgggatt ccggattgcg gcggccgatc tcgagatccg
    23581 cggcgcgggc aacctgctcg gcaaacagca gtcgggacac atcgcggcca tcggactgga
    23641 tctctacatg caaatggtcg agcaggccgt gcagcggttg aagggacaga tcgtcgaaga
    23701 agaaccggac ccgacactcc gtctcaacgt ctccgctttt atcccggaag actatgtggc
    23761 ggaccctcat catcggctgt cgctctacaa acggctctca tcctccaccc aggtgggcga
    23821 ccttgcgctg ctccatggtg aaatacagga tcgattcggt ccaccccctg agccggtcga
    23881 acggctgttc gaggtgatgc agatccggtt gcaggccaag gccttgcggc tggtctcgat
    23941 cgaactgaaa gccggggcgg ttgcggtcac gctcgatggg aagagccgcg taccgcagac
    24001 ggccattcag cagctgatgg accgatataa gacgcgtatc cgttttcttt ccccactggc
    24061 gtttgagctc caaatgccgc atcaggactg ggcttccatc tttcccgaac tcaccgcaac
    24121 cttgcaaagc ctgggagtct gtgataccaa caccagagcg acatcgcctt cgcccacgta
    24181 actccacaag gattctcatc tgcatgcatc gacgaccgac tgaatacgcc actgcctccg
    24241 ccgtcctaca catgtgtctg atgatgatgg cggtcggggg cctgctcctg ccgctggtgg
    24301 gctgcacgcc gccgcaagcc gaagagcgtg tggtggcatt cgtcaacggc aaaccgatca
    24361 cacaaaccga gttcgaccac gagtgggcag atttgcccga cgccacgaag gcgcgctatg
    24421 agaaggaagg gggcaggcag gtcttcttga aggaactggt cgaccacgaa atgctcttac
    24481 aggaggctcg caggcaaggg ctcgatctgg atgacgcgat ccgcgatcgg gtgcggcgct
    24541 acaaggaaaa actgctgatc gatgagctgt tgaaagaccg gatgaagacg acggtcgagc
    24601 tcacgaaaga ggaactcgac cggtattacg agcagcatgc cagtgagctc ttgacgccgc
    24661 tcaaggtgcg ggtggcccaa atgctgcttc ccaattatcc tgcagccaag gacctggaaa
    24721 cgcaggtcaa ccgggggggc gatttcggca agtttgccca gcgctactcg atcgattaca
    24781 agacgaaagc caaaggcggg gaccttgggc cgtatcgcaa ggggctggtc atccccgaag
    24841 tggatgacgc catccggacg ctcaaacccg gcatgatcag cgcgcccatc aaggcggaga
    24901 gcggctacta tctcgtcatg atcacggcgc tcgagccgga aatcatccag gccgacctcg
    24961 cgaagcgcga acgacttcga caggaactgc tgtatctcaa acgcaagcag tatttcgaca
    25021 gtgtgatcgc tgaaatcaaa ggcaaggctg tcgtgcgact cgccgatggg tcacgcttca
    25081 gcggggacgg tctgactaag cgctagcaga cctgctgaaa acgcccgaca ttacacccac
    25141 ccttgcccga cgcgcagagg cgcgtccctc cactaagctg tgttttcgcg tcgcctatcc
    25201 ttctcttccg catgcgacgt tggatcttgt acaatcaccc tccacgtcgc gcgacagcgt
    25261 caaccccttc gcgcccacgg accatgagca cctcgttccg cacagccact tggttacgcc
    25321 tcgggatctc tgcactgctg ggcctcagct ctgttggagc gccatttccg tcttttgccg
    25381 ccaaagtcga agaccgcatc gcggcaatcg tcaactcgga cttgatcatg ctgtccgaag
    25441 tcaggcggga gctggcgccg gagcgggagc ggctggagaa gcactatcgg ggggaggagt
    25501 tgtcccgcag actccaaatg gcagaagcga tggccttgac caccatgatc gaacgacgac
    25561 tgcagctcca agaggcaaaa gcccgtagtg tggaagtggg agaccaggag gtcaaacagg
    25621 cggtcaagca gttgattcaa cagggcgaaa aaatcgacga caaagatccg gtcagtacga
    25681 atagcgttcg ggatcagttg acgctgctca aggtcgtcga tcgcgaagtc cggagcggcg
    25741 tgatggtcgc cgacccggaa atgaaacggt actatcaaga gcaccgtgat cggtttgcgc
    25801 ttcccgaaga atatacgttg agccagattc tcatcaaacc ccgctctccc gacgaggtcg
    25861 cagacgcgag agcgaagggt cgcgaggtaa ccgctctgct gaagcaagga gaatcgtttg
    25921 aggatctggc gcttcgctat tcggacggac cgacggcgtc acgagggggg cgattggggc
    25981 tggtccgtca aggcgaattg ctgcccgcga tcgagcgagg cgtctcgaat ctcgttccgg
    26041 ggggcatttc ggacatcatt gaaagcccgg agggcttgca catcgtgcgg ttggacgata
    26101 agaaaccgaa acaattccgg ccgtttgaag aagtgaggca ggaaatccaa gcgctcgtct
    26161 ttcagcaaaa aagcgaggac atgtttcaat cgtggctggc cgacttgaaa aacaaggcct
    26221 atatcgaaat caagtttgac agtgctcctt taagacagac cacaagcgtt cccgcccctc
    26281 ccccgtcacc gacgagtaag ccatgaacgt cgggctcccc gatagaccaa gtccttcctg
    26341 gagccgccga agcgccgcca cacgctcgcc ggctttcaat ttgtcggcct tggttgcgac
    26401 gacgaccggc tcatagccga ttgccctgag ccagagaagc gtctcggcat cctgcgggct
    26461 gatgatgcgg ctttccacca gcatgacgat gcagacctgc gcgtcccgtc cgaggtacgc
    26521 ctcgatcaac ggcgcccatt gggctcgttc cgttttcgat accttcgcat agccgtagcc
    26581 gggaagatcg gccagcacaa accgcgacaa ttgtggatcg tcagtgacga ccgcaaacag
    26641 gttgattgcc cgcgtcttgc caggcgtgcg gctcaccttc gccagccctt tccgctgcag
    26701 caatgaattg atgagcgagg atttgcccac gttggagcgt ccaacgaagg cgatttcggg
    26761 tatggaatcc gtcggaaatt gtgctgcccc gacgcaactt ctgataaaat cggccttcag
    26821 aattttcatg agcggtcctc tgcacttgtc atacggttcc cgcatgcgga agtcaaccaa
    26881 acggcgcggc agacccctgt ggctgatact ctgcgcgctg gtggccattc cgtgcgccgc
    26941 cctggcattg ctctggctgg tgacgcttcc cgacgtgtcc tcgctgcgat cggtcaaccc
    27001 gacaaccacc gccttgatcg aagcgcgcca gatcgaagcc gaggcgaagg gcctgagaat
    27061 cggccggcgg tggatttggg tgccgctgtc ccgcatctcg ccgtacctcc gccgtgcggt
    27121 cgtggcagcg gaagacgcct cattctttac gcacgaagga ttcgactggg aaggcatgaa
    27181 ggacgcggcg atctacgacc tcgaaaaggg agaactcaaa cggggcggca gcaccatcac
    27241 ccagcagctc gcaaaaaatc tgtatctctc ttcggagcgc tcgctcctcc gaaaagctca
    27301 ggaagcgctg atcacccggt cgctggagca tgagttgtcg aaagagcgca tcctcgagtt
    27361 gtacctcaac gtggccgagt ggggcaccgg tgtctacggc gccgaagcgg cctcccggca
    27421 ccacttcaat aaaccggcgc gtgacctgac cgaagacgag gccgcttggc tggccgccat
    27481 tcttccgtcg ccgcgccgct acgatccgct tcgaaaaaca catgcgctgg ccaagcgcta
    27541 cgagcgggtg ctccatcgga tggcccagaa ctcacggcac cagatccgga tggattaacg
    27601 gacctccatg gttcatctcg gtgaggggga atggagaaaa tctcaccgcc cggcgtacat
    27661 tgcgacgcag accggtccgg tgaaaaccct tggaagtccg ttcttgatca tgggtgggat
    27721 gcgcgccggg ttctcacagt cagtcggcgg gcgaggcgtc gcccacagca cgaaaatcgc
    27781 gctcaccgaa aatggcggac tcatgggtgt aatgtttgaa ctcgagcagg ttctcggagg
    27841 gatcctgaag aaagaatgta tggtgctcga tgcgcgtgcc gggaaaccgg actcgcggct
    27901 gttgaaaaaa ccgcagcccc ttggcttttg cccggtccgc caatgcctgc caatcgtgtt
    27961 tcgagagaaa caccaagccg aaatgacgcg ggtaaattcc tctctgctca ctcggagaac
    28021 cgtcaacgac atgggcgacc agttggtgat cggccagtcc gaacgtcacg gcttgggcgg
    28081 attcgcgtcc gagcttgcac ccgagtccct cgacatagaa ctgcttggcc tggttgacat
    28141 cgtggattgg gaaagccagg tgaaacagga tgtccttcat gtccaagacc ctttgactgt
    28201 cggtcgcgcc gagccgttcc accacccggc ggccatgtgc ggagtcacat gggcgatcgt
    28261 gaatcgcccg tccggtgcca ccaccaatgt accagccgca cccgcgatcc gcgagaccag
    28321 ttcgcgcaag accatcctgg ccgccgtgga cggagacttt cctgccgcga gccgctcgca
    28381 gatcgccttg gcgaccgcaa ggcggatgat gccttcgcct tgacccgtca tcgacaccgc
    28441 gccgcagcga ttatccgcgt agactccgca tccgatcagg ggcgtgtccc ccacccggcc
    28501 gggcaccatg acctcgatcc ctccagtcga ggctccagcc gcgacggtgc gagcgcgatc
    28561 gagggccacg gcaccgacgg tcccatgacg atccttcacg cttcccttgc cggcgaggat
    28621 ccggtcatag ccgagctgac gacgtcttcc aggcggacga tgtcgctcga gaccaaaatg
    28681 ctgcgcaaat ctgccggcga ggggccccgc taacaacacg tgagtcgtct cctccatcac
    28741 aagcctggct gcggtgatcg gatggacgaa gccttccagc gaagccaccg ccccggccct
    28801 gagaccggcc ccttccatga gagaggcatc catccgttgg atgccgtcga gttgccgctt
    28861 cgagccgcgg cccgcattga agagaccgga ttgctcaaga aagccgatcg tgacttcgac
    28921 cgcgactaca gccggacctc cctgctcaag aatcgcatag ccggtggcga gcgcatcggc
    28981 gaggcaggac gactggcgtc tggttattgg acgccggccg gcaccgccgt gtgccagaag
    29041 aaggggatga ggacgggtca attgaggagg agatccttgg cctgttggta ggccggattt
    29101 ttcatacggt caaggtcgga acggacgaat cccagccccg tgacgcacag ctcaaagttg
    29161 tcagaaagtt ttgcgaaaag aggacgttcg tcctgggcag gctggtccgc catttgcgca
    29221 acgatgccgt aggaacgttt gcctgtcttc acgtaatcca ccaggaaatc cttatggtag
    29281 accaacccgg cggtcttcag ccgacgcagg tattccggaa agagcccggc catgaacaac
    29341 gtgaagtcgc cgatgtgctg gtggacgtcc cgttcccgat ccaatgactg cgcctcaagg
    29401 agcacctcgg attcgaacag caggtccacg acagaatcga ccggttggcc ctggcggttt
    29461 ctgattttaa acagttgatc ggtccgggtg aactcaacga gcaaattggt cacgtaggtg
    29521 gtcacattga gatcgggcca gccgagatgt tccgtgaagg ttttctcggt caaggcgccg
    29581 aagagctgac gcagcggatg tcgagcgggg attgacgttc ccatctcacc tctcactgga
    29641 tcacaagtag ttagacctat tatagcagag tctgacccga agaccgctta aaagccgttc
    29701 cgactgtcta acacaaaggt gacggggccg tcgttcacca actcgacctt catgtgagcg
    29761 gcaaacacgc ccatctcgac ctgcgtcccc tgcaccttca attcttttga cacacgctca
    29821 taccagtgtc tggcttcatc gggcggcgcg gcatcctcaa agccggggcg ccgtccgctt
    29881 ttggtgttgc ccaagagcgt gaattgggac accaggagga taccgcctcc cgtctcggtc
    29941 agcgaccggt tcattttgcc cgcgtcatcg gcaaagattc gcaggccccg caatttctcg
    30001 accatgaacc ggcaatcccc atctccgtcg cccttcgcca caccaagcag gacaaccagt
    30061 ccggctccga ttgagccgac gacttgcccc tccacttcca ccgatgcgcg agtcacccgc
    30121 tggatgaccg ctttcatcgg tctcgatccg catggcgaac ccgggatagc gagccttcca
    30181 gggccaataa cttgcgtttg gccggcagcc cacatgaaaa gccaccgagc ccggcgtcct
    30241 gggcaatgat ccgatggcac ggcaagacga tgggcagggg attggcgccg actgccccac
    30301 ccaccgctct cgcatactgt accccgccga cccgtgaggc cagacctcgg taagaccata
    30361 gttgaccata ggggatcgcc cctagcttct tccatacgcg ctgctgaaag gcggttcctc
    30421 gcgagaggtc aaggggaaga tcgaaggacg tccggctccc ggccaaatac tccgtcaatt
    30481 gcttgcgcgc ttcccgtaga agagcagacg aagatgtgcc gttgaagccg cttccccctt
    30541 ttcccaaggc cgtcgtcacg gccaccttcg aggtctgcgg cagcacgatg gcggcaaggc
    30601 cttctcgtga ttcggccacg cccatccatc cccaactcga cttgaaaatc aacgcgcgtg
    30661 tcatcagatc ctcctagcgg ccgatgagac ggccgaacct tttcttgacc atgctccaca
    30721 ggagatcgag ttcttcggac cgcatcagcg catggacgcc gagatagccg ctcacactca
    30781 atccgatggc gaccgtcagc atgacggatt ttgccgccca ctcgccctca tgcgtccaca
    30841 cctgtgcgcc ggccacccac aggcaggaca ggaccagcgg aatgcacgca accagcacgc
    30901 gtccggccga acggcctacc aatccccacg cgacaccgcc gagacgacgg ttgagcaagg
    30961 cgatcaaaat gattccgttc accatcccgg cgagcgccgt ggctaacgcc agaccagcgg
    31021 cacctaaggg agacatcaga agcaaggaga agacaatgtt ggcggctacg gcaatggcgc
    31081 ccgagatggc aggagtggtg gtgtcctgaa gcgagtaaaa cgccgccaca atgatgcgca
    31141 cgcccccgaa tgcccaaagg cccacggcat aacaaagcaa ggccaaggcg gtttcggcag
    31201 tgtcatgcgc cgtaaacgtg ccgtgctcga agaacacgtg cacgatcggc gtccgcagac
    31261 aaatcagccc gagcatcgcc ggaaacatga tgaacacaat catccgcagt ccgaatccga
    31321 acgtggtccg gagttcctcc atggcaccgc gggccgcctg ggccgagagg gtaggcagaa
    31381 tggccatggc aagcgccacg ccgaagatcc ccaagggaaa ctgcaccagc cgcatgccgt
    31441 agaacaaata ggtcggcgcg ccgctgatga aggatgccag gatcgtgctg atcgtcaaat
    31501 tgacttgtgt caccgacagc ccgagcagcg acggcagcat cagccggccg atccgcttca
    31561 cgccgggatg gcctggctca aagcggatgc cgaataaaaa ccctctcgcc ttgaggctgg
    31621 gaatctgcat gaggaactga gccgctcctc cggccacgac gccgatcgcc acaccgacaa
    31681 tcggttcctc taattgcggg gacagaaaca gcgcgcatcc gatgatgcac acgttcagga
    31741 agagcgccgc ataggccggg acggcaaagg cccggagtga attcaagatc cccatcgcca
    31801 gcgcggcgag actgatgaag agaagatagg gaaacatgat acgggccagg accgtcgtga
    31861 gcgcgagttt ctccggcgag gcatgaaaac ccggtgccag agcctggacg agccagggcg
    31921 cgatgagaat gccgagcaat gtcacgaggg tcacgatcgt gagcagcgtg gtgaagaccg
    31981 cgctcgccaa ttcccacgcc tcacgtttgg atttgagcga gtgatattcc gtaaacaccg
    32041 gaataaaggc ggcggacatc gagccttccg cgaatagctc gcgcagcaga ctggggatac
    32101 ggaaggcgac gtagaaagcg tcagcagccg gcgtggcacc gaacaggcgt gcgagaacca
    32161 tgtcccggat gaaaccgagg atgcggctcg caaaggtggc aaccccgatg acccccgcgg
    32221 ctttcaccag tgaatggttt tcgctctggg atgttttcga gagagccgtg gcggtatcag
    32281 acatctgggc ggattctaac ggaacgctgc cgcgcagtct attgaacagc gcgttatggt
    32341 tcctgcacga gtcggtttga gagttcgtcg ggattgtcat tcgacgacgc aggatacgcg
    32401 agcgccagca ggactccgca gcgttcgatg ccggcgcaga acccgcccgc gatgtcgtcc
    32461 gctttcagcc tcgtcaccac ggcttccacc acctcgttcc attgtgccgc cgtgatccgc
    32521 gggccgattc cggagtccgg caagacatac acatgccgtt ccatcagcga gaccatgagc
    32581 aagacggcgg tgcggtcgcg agtgcggaag aggctgtgtt gtgcgaacgc gcgctccgcc
    32641 ctcagttgca ctttgctgcg cagccgttcg gttgaagtga ccgcccggat gaccggatcg
    32701 aacgtaccga gccacgagcc gatcacatac cccatcaggg tcaagacgat gagccacgcg
    32761 gcattggctg catgccaacc ccatggcacc cagaacgcct cgctcatcag caggccggtg
    32821 agtaccatca gtgccaaggc caggccggcg cgatgttgcg catcatggta aagtccggag
    32881 cgtgcgacga gcatcggtac gatctctgcg ctcgtcagcc gttcagcggc acgaaccgca
    32941 tcactgatgc gggccttgtc ctcgtcagtt aatggccccg ccttaccagt cgccactggc
    33001 ccctcctccc ccgaaatccc cgccgccccc gctgaatcct ccgtggtcaa acgaaccgcc
    33061 gctccatccg ccccgactgc tacggtacca agtccaatcg tcaaatcccc gccgtcgccc
    33121 gggtcccttg gcactcagcg ccatccagag caggacagcg gtcaatcccg ctgcagctac
    33181 tccccataga atgctcgccg cctgtgccac catgaacgtc aacacagcgc ccagcaacgc
    33241 acgggcgcgc cgcagacctt ggctcagcac taatccagcc agaaacccca cgaagacacc
    33301 gatgacgaca tactggaagg cactcgactg tagaagctgc cgatcacgag tgggcgactc
    33361 agccgcctga taggtacctt cgatcgtccg cagtatagcg tcggtaccgg cccgcactcc
    33421 ggccggaata tcaccggcct tgaaccgcgg cacgatctca tggcgaatga tgtgggctga
    33481 acgcaagtcg gtcagcgtcc cttcgagccc atagccgacc tcaatccgaa gcttccgctc
    33541 cttgagcgcg atcagcaaga ggacgccatt gtccgtccct ttgcgcccga gcctccatgt
    33601 ggtcgcgacg cgatgggaat attcctccac cggctctccc tcgagagacg ggagtatcag
    33661 caccgcgacc tgattcccgg tttgctgctc gtgagcttcg agccgagcac tcaactgttc
    33721 agagtcgtgg gcaggcaacg caccggcaag gtccacaatg cgacccgtca acggcgggac
    33781 ctccagcgcc gagaggagca gcggattcag cagaaatccc aagaagacga cgagccgcgc
    33841 cggctctcgc cagacacgac tcatacagag gagagacatc atgcaggcag gtctagaact
    33901 tgacctccgg cggcttggcg acggcttttt catctgcgac cgagaagttg ggccgctcgt
    33961 ccagatggag gatgaatttc gcggtcaaat tggtcggaaa ataccgcacc atcttgttgt
    34021 attccgcgac gcgatcgatg tatcgcttgc gagccacggt gatccggttt tcggtgcctt
    34081 ccagctgact ttggagatcg cggaaattct gatcggcctt taagttggga tagttttccg
    34141 caatggcaat gagacggccg agggcagacg tcagaccggc ctgcgctttt tgaaattgct
    34201 cgaacgccgc cgggtccttc agcatctcgg gggtgacttg aatgccggtg gcgtgcgccc
    34261 tcgcctgaac cacgccttcg agtgtctctt tttcatgcgc ggcgtatcct ttcaccgtcg
    34321 ccacaagatt gggaatgagg tcggctctcc gctggtattg attgaccact tcgctccagg
    34381 ccgccttggt gtcttcatcg agtccctgga gatcgttata gccgcacccc cagagaccgc
    34441 cggtcatgag tacgcccagg acgatcatgt aaaccctgcc catagctctc ctccttgtga
    34501 tgaacgtgca aacggatcat gctatcattg gtcgaacacg gaagatagcg ggagacttca
    34561 tgtcactgga acgctcatcg aatcaagcgg gaggatttca gctcaggcac cgtgtgctgg
    34621 gcatctatca gggaagtgcg attgagctgg ccttctggca tccttcgtcg ggcatgcccg
    34681 aatatgggct ctgccggttc tcgaccgagg acaaggcgca ggcgctggtc gatttcttga
    34741 catccccggc gtgcccggaa ccgttcgatc ccaaggacct gacgatcgaa ccgtatgacc
    34801 tcgtcgaaca tgaacgactg acgactgact accccctgcc ttccgcctgg gaacaaccga
    34861 cataacgccg tgtcatgtgc cggcggcgag aatctgcgcg ctggatggga gaagcagagg
    34921 cgtgctagct tttgaccggg acggccaggg ctgtcgattt ctgcagatga tcggcggtct
    34981 gcgcctgaat gaaggcgagc aggcgcttgt tctcctgggc cgcccgcaaa aacttgaagg
    35041 cgatgccgcg cggactcacc gagcggacca tcgcggccac ttccagcggt tgctcgtgtg
    35101 atcccgtggc gatctccagg tagaagatat cgtcgacccg cacctgcgtt tcgcttctga
    35161 tgatgcagcc gcccatcgac agatcgagca ccgtgccctc gccgcggact ttcccaccgg
    35221 aaaacgagag atacagccgc acgggaatcc ggagatgctc gcgacggtcg atcggatgac
    35281 gcgggcggtc caatccaatg cgggaggcca gaaagcggca actgcagtgc tggcaggaga
    35341 agggggacag cagaaagatc gccgccaccc gttcgctcgc cgagagcggc ctggcgcgca
    35401 gcacctgttc atggcggcat tggggacaat tcagcgagcg acccatagag ttgttcagtc
    35461 agaacctgcc acaggagcgg tgatatctca cttactcatc gatagtgcat gtcccccgat
    35521 ctgtcaagcg aagaggcgta aacagccaca cacacgtgac gtcagcgcag cgctccatcg
    35581 ccttcgggcc acgtgggaga tcagcgccga gagtgaacgg cgaggttgtc tcagtgagat
    35641 tcgtcgtgag gatctgccgg ctgttccgcg aggggactcg gctcttcgat ggtggctcgg
    35701 agaaattccc ggttgagctt cgcgatgaat cgcacgctga tggactttgg gcagaccgct
    35761 tcgcactcgt attggttgcc gcaggacccg aacccttcgc gatccatggc ggccaccatg
    35821 gccttggccc gtttcgtccg ctccggctgg ccttggggta acaaggcaag gtgcgagact
    35881 ttggcggcaa cgaacagcat ggccgaggca tttttgcacg cagcgacgca cgcgccacat
    35941 gaaatgcaag aggcggcatc catggccgtc tcggcctggt ccttcccgat caacaacacg
    36001 ttgccgtcca cggctccgcc cgtattgacg gagatatatc cccccgcttg catgatgcgg
    36061 tcaaatgcgc ttcgatccac gacgagatcc ttgatgatcg gaaaggcctt tgcgcgccaa
    36121 ggttctatgg tgatcgtggc gccgtcggga aatcgacgca tgtagagttg gcagacggtg
    36181 ccgcgatccg gaccatgcgg cacaccgtcg atgacgagcg agcagctgcc gcagatccct
    36241 tcgcggcagt catactcgaa tgccaccggc tcttcatcct tcagggtgag ctgttgattg
    36301 acgacgtcca gcatttcgat aaaggacatg gacgggctga tgtcccggac ctggtaggtc
    36361 accaagcggc ctttgtcggc cggtccgttt tgacgccaga ttttcagcgt gaacgtcatc
    36421 gtcgcccttc cgtttccttc tgcttcaatc acatccactc gtgaccgagc gcgcaccgca
    36481 ccggactatt gataggttcg catggtcggc ttcacgaatt cgaacaccaa tggttcctta
    36541 tggagcacgg gccggccgtt cggcacgtat tcccaggccg acacatacga aaagttcgcg
    36601 tcgtcgcgga gcggctcgcc aagcggcgtc tgatgttctt cgcgaaaatg cgctccgcac
    36661 gattcttcgc gatgcagcgc gtcatagcac agcagctcgg cagattcgag aaaatcggcc
    36721 acccgacccg catattccag tgcctgattg aatgttgccc cggatccaag gaccgctact
    36781 tcgtgccaga actcctcgcg caaggccgga atggcttcga gcgccttctt gagccccgct
    36841 tcctcgcggg ccatcccgca cagatcccac atcaggcgtc ccaggctacg atggtatgac
    36901 gcggcggttt ggcgtccacg gccccgaagc agacgcgcga cccggttggt cacacgctct
    36961 tcggccgccc gcaggtctgg atgacccgtc ccgacgggac cactcttcgt ggtcgccaga
    37021 taatgaccga tcgtataggg aagaatgaaa tagccgtccg ccagaccctg catcagcgag
    37081 ctggcgccga gtcgattggc gccgtggtcg gcaaaattgg cttctccgat cacgaagaga
    37141 ccgggaaccg tactcatcaa attataatcg acccatagcc cacccatcgt gtaatgcggc
    37201 gccggataaa tacgcatggg cgttttgtac gcgtcttcac ccgtaatgcg ctgatacatc
    37261 tcgaacagat tgccgtagcg ttcctggacc acctcgagac cttgctggga aatggcgtcg
    37321 gacaaatcca agtacacccc tagcccgccc ggtccgaccc cgcagccgtc gtcgcagatg
    37381 tgcttcaccg cccgtgaggc cagatcgcgg ggggcgagat tgccaaagcg tggatagcgc
    37441 cgttccagaa aataatcccg ctccggttcg ggaatctgct cgggagaccg gctgtccccc
    37501 ttctgcttcg gcacccacaa ccgcccatcg ttgcggagcg attccgacat caacgtcagc
    37561 ttggcctgat aggcttcgct cggtggaatc gccgtcggat ggatctgtgt gaagcaggga
    37621 ttcgcgaagg cggccccctg tttccaagcc cggtaggtcg cggtcacatt gctgccgctc
    37681 gcgttcgtcg agagaaaaaa aatgttgctg tatccgcccg tggccaacac caccgcctcc
    37741 gcggtatggg accggatcgc tccggtcacg agatcccgca cgacgattcc acgggcgtgt
    37801 ccgtcgacga cgatcagatc cagcatctcg gtgcgcgggt gcatccggac ctgcccgcgg
    37861 tcgatctgcc atgaaagggc cgaataggcg ccgagcagga gctgttggcc ggtctgtccc
    37921 cggcagtaaa atgtgcggga cacctgcacg ccgccgaacg aacgattggc taattgtccg
    37981 ccgtactccc gggcgaacgg cacgccgagc gccacgcact gatcgatgat ctgcatgctg
    38041 acctgtgcca agcgatagac attcgcttca cgggaacgga agtcgccgcc cttgatcgtg
    38101 tcgtagaaga ggcgctcgac gctgtcgcca tcatcctgat aattcttggc cgcattgatg
    38161 ccgccctgcg ccgcgatact gtgcgcccgc cgcggactat cctggaagca gaagcagtcc
    38221 acctggtagc cgagctgccc gagcgttgac gccgcgctgg cgccggccaa gccggaaccg
    38281 acgacgatga ttccgacctt cctcttgttc gtcggactca cgagcttttc actgaaccgg
    38341 tggcggtccc atttcgtctc gagcgggcca tgcggcactt tagaatcgag ggtgatcacg
    38401 cgtgcaccag ccccaacgcg atcgcgagga taatggccac atttcccacg accaccagca
    38461 atgccaccag cggcccggca cctcggaaca gacgatccag catgggatgt tccaagccca
    38521 aggtctgcag gctgctcgac agggcgtggc tcaaatgcag tcccaacacg gcttggccgg
    38581 cgaggtaaat cgccaccagc agcggatttt ggaaggcgtg cacgatattg ccgaagacgt
    38641 cacgctggcc gcggagatcc acgcgattat ggaactcggg gtccagccag cctgccgtca
    38701 aatgcaccag atgaaagaca atgaacagga ccaggaggct gccggtcagc gtcatggtgc
    38761 gggaggccag ggaggcccga cgatagtgat ggaccacgta accggcagga cgcgcgcgcc
    38821 ggttttgaat cgccaggcgg accgacagac cgatatgcag gccgacgatg gcgagcaacg
    38881 cgacgcgcgc actccagagg gccatgggca tgtcgcgcaa gaatgcggca tacgcattca
    38941 gggcgtcctg cccttggaat acttgcaggt ttccgagcat gtgaaagacg acgaatccgg
    39001 ccagtcccaa tcccgaaagg gcaatgagga ccttgctgcc gagggaggtg agaatgtgac
    39061 cgaagaatcg aatcatgggc aacgaatcaa atcgcgccgc tttcttcact tgtactccgc
    39121 tcggttccat agctcaagtg tctgcgggaa acgtgatcac gagacggacg ccgcagcggc
    39181 gcaaccggcc aaatggccgg tcgcccaggc ccattgaaaa ttgaaaccgc cgatgcgtcc
    39241 gtcacaatcc agtatctccc cgatcaaata cagaccgcgc accactttcg actccatcgt
    39301 ccggaaattg atttcttcaa gcgggacacc tcccgccgtc acctccgcaa cgttccatcc
    39361 acgatctccg gtcgccggaa gggccagccg ggtgaggacg ctcagcaacc ggtcccgttc
    39421 gcttcgcggc atttgtgccg ccgcccgctg tgggttgcaa ccaaccgccg cacacactgc
    39481 ttgggcaaac cgttcaggca cgacgagcgc caaggtcttc accacagacc ttcgcggact
    39541 ctccgatgcc tgcatgatga accagtgcct agcctgctcg aacgtccgcc ccggaagcac
    39601 attgagatag atctcggcgt gctcgccccg ttccctggcc aacgtccaca cgcggctggc
    39661 atccatgacc accggcccgc tgacgccgaa atgggtccag agcaaactgc cggtccggac
    39721 gtcgacagcc ttgccggcca ggactgtcat gagcgtcacc tcttgcgaca cgccggaaag
    39781 ctcccgatga aacatcttat cgtccaagag caatggaacc aaggcgggaa cggtcgcggt
    39841 caccgaatgg ccgagatcgt tggccaagac atcgccggaa ccatcgcttc ccgatttagg
    39901 aagcgagcga cctcccgtgg cgagaatgag agtcctggtg tgaatcgctc cccgtgagtg
    39961 aacgactgtg aaccctttcc tcccgtcgag accgcattcg atgcggctca cccgatggcc
    40021 tgtgaacatg ttgatgtcca gggcctggca acggttgagc agcgcgttca gcacggtccg
    40081 cgccttatcg gtggtgggaa agagcttgcc ggtgtcttcg cgcttgagtt cgacgcccaa
    40141 tgcgtgaaac cagtcgatgg tctctcgaac cggaaaggca gcgagcacat ttttgatgat
    40201 gcgccgattg ccgaaaaaat cgctggccga gaccgcttcg tgggtgacgt tgcatcgacc
    40261 gccacccgag atgaggatct tcgcgccaag tgtctttgcc ccatcgagga ggaccacgtg
    40321 ccgaccgcct attcgttcgg cggcggcgat tgccgcggca aggccagcgg ctccccctcc
    40381 gacgatcacg agatccgccg catccatcag gcgcacctta ctcgagactg gagcggcacg
    40441 gcgcctgtcg cgtgatcggt gcccggccgt gtccctccag gaacgcacct gcatgtgcag
    40501 acacgggagg catcaggcca tgcattggac taacaggcgt aagagctctt cgacttcgga
    40561 ttgttcgtgg aggaaaaagc gcgcgcggga ctcggcctgt ctgccgacac ggacgcccat
    40621 cgtgaccccg tccggcaacg agaacacggt ttcatccgtt tcctcatctc caatataaaa
    40681 gagtccggtt cggccgagat ggttcatcag ggccaacgcg gcttcaccct tcccgcccag
    40741 gccgggaggc aacgcgttga tcgaatactt gccttcgatc agtctcggct ccggagacag
    40801 ccgccggagc caggtgagtg aagtccttac ggcgtctgcc gggtcttggg caaggcgcac
    40861 atgaaccgtc aatgaatagc gcttgtcctc gatctccgca ccgagcagta tcatcggcgc
    40921 gagcgtgcgc tcgagttcgc gcttccagtc ggcacagatt cgctcggctt ccgtcagcgt
    40981 gctgcgagac gtcaacggac tttcgatgcc gtggttgccg atcacatgag gcacgattcc
    41041 gttcgtccgc agcgacacgt cggcgagcgc ccttcccgac acgatggcgc acggcacccg
    41101 cttggccaac tcacgcaacc attctgccac gccaagcgtc agagtaacgg catggcgatc
    41161 gcttgagatc ggcgcaagcg tgccgtcgaa gtcgaaggca taaagaagcg gtctggacgc
    41221 ggcgtcgcgc aatgcacgaa ggccggcttc cgagagggca tggatcacgc ctgacctccc
    41281 gtcgtggtt
//
LOCUS       DASSCQ010000148         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1387257, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000148 DASSCQ010000000
VERSION     DASSCQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387257"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFH05_10455"
     CDS             complement(<1..249)
                     /locus_tag="VFH05_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678683.1"
                     /translation="MVDRWLRGFLTGTVVLASLLAISTRAQSQLDPLKDPSAQSGASS
                     ESDRELGPSDPLESANEPEDRLVILPEIKREGERFYLSS"
     gene            346..1050
                     /locus_tag="VFH05_10460"
     CDS             346..1050
                     /locus_tag="VFH05_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6678684.1"
                     /translation="MLHFGAMRSLAVLNQHIITCTACPRLVTYRQAVAQEKRRQYREW
                     IYWGRPVPGFGDPAARLYVLGLAPAAHGGNRTGRIFTGDRSGDWLYEALHHFGFANQA
                     TSTHRDDGLALSDCYIGATVRCAPPDNKPMPEEFERCRPFIREELRLLQKMRVVVVLG
                     KIAFDHYLKACREEGRMLPSPLPTFGHGTVYRLPWHVTLVGSYHPSQQNTFTGKLTKP
                     MFHAVFTLAKSAIERG"
     gene            complement(1113..1760)
                     /gene="fsa"
                     /locus_tag="VFH05_10465"
     CDS             complement(1113..1760)
                     /gene="fsa"
                     /locus_tag="VFH05_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET6678685.1"
                     /translation="MKFYLDTASVKEIQEAASLGLLDGVTTNPSLVAKEGRVFREVLL
                     EICNIVDGPISAEVVSIEADAMVKEGRELAKIHKNIVVKVPLIAEGLKATKRLAAEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDISSNGMELIRQIVTIYKNYDYKTFI
                     LVASVRHPQHVVEAALAGGHICTMPFAIFQQMVKHPLTDMGLKKFLADWDAQNKK"
     gene            1985..2299
                     /locus_tag="VFH05_10470"
     CDS             1985..2299
                     /locus_tag="VFH05_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678686.1"
                     /translation="MTRRWLLALIGCLFLSACGGNNPYLEQSLKPAELQGKDKSWFLK
                     NWGEPSGKAPRFFGGETWTYFRIAGGKSGPPLFNFSPNQCEITLKFDKEEKLSDYSYS
                     GC"
BASE COUNT          610 a          827 c          775 g          605 t
ORIGIN      
        1 cgaactaaga taaaatcgtt cgccttctcg cttgatctcc ggaaggatca cgaggcgatc
       61 ttccggctcg ttggcgctct cgaggggatc ggaaggtccc agctcacggt ccgattcgct
      121 gctcgctccg gattgagccg atgggtcttt caagggatcc agctgggact gcgcccgtgt
      181 ggaaatcgca aggagggacg ccaagaccac tgtgcctgtc agaaaaccgc gcaaccaccg
      241 gtcgaccata cgcacctatc ctccgagttg ttcgacctga tctctttcag tcggcatgct
      301 accagcaagc gacacggtcg gcaagcagga tcggctttca ctaacgtgct acacttcggc
      361 gccatgcgat cgctcgccgt tctcaatcag cacatcatca cctgcactgc ctgccctcgt
      421 ctggtgacct atcggcaggc cgttgcccaa gaaaagcgac gacagtatcg agagtggatc
      481 tactggggac gaccggttcc gggattcggc gatccggcgg cgcgcctgta tgtgttgggg
      541 ctggcgccgg cagcccatgg cggcaatcga acgggccgaa tctttaccgg tgatcgaagc
      601 ggagactggc tttacgaggc gctccatcac ttcggatttg cgaaccaggc gacctcgacg
      661 cacagagacg acggccttgc attgtcggat tgttacatcg gggcaaccgt tcgctgcgcc
      721 ccaccggaca ataagcctat gccggaagag ttcgaacggt gccgtccgtt tatcagggag
      781 gagttacgac tgttgcaaaa aatgcgggtg gtcgtggtcc tcggaaaaat cgcctttgat
      841 cactatctga aagcgtgccg cgaggagggg cgcatgcttc cttcgcccct tcccacgttc
      901 ggtcatggaa cggtctaccg ccttccgtgg cacgtcacgc tggtcggctc ctatcatccc
      961 agtcaacaaa ataccttcac cgggaaactg acgaagccga tgttccacgc ggtattcacg
     1021 ctggcgaaaa gcgccatcga gcgtgggtaa agcgcacgtc tcggcgtgcg ccgggtcggg
     1081 cgggtgagaa tagagcaggc ctaacaaccc ctttacttct tgttctgcgc gtcccaatcc
     1141 gcgagaaact tcttcaagcc catatcggtc agcggatgct tcaccatctg ctggaagatg
     1201 gcaaatggca tcgtgcaaat atgcccgccg gctaatgcag cttcgacgac gtgctgggga
     1261 tgccggacgc ttgcaaccag gataaaggtt ttataatcat agtttttata gatcgtgacg
     1321 atctgtcgga tgagctccat gccgttagag ctgatgtcgt cgagccggcc gatgaacggc
     1381 gagacgcacc aggcgccggc cttggctgcc agcaaagctt gcgtcggtga aaagcatagg
     1441 gtgacgttca ctctgattcc ttcggcagcc agccgtttcg tcgctttgag cccttcggca
     1501 atcaagggca ctttgaccac gatattcttg tgaattttgg ccaattcacg cccttccttg
     1561 accatggcgt cggcctcgat gctgacgacc tcggcgctga tcggcccgtc gacgatatta
     1621 cagatttcga gaagcacctc gcgaaacacg cgtccttcct tcgctacgag agacggattg
     1681 gtggtgacgc cgtccagcaa tcccaggctc gccgcttcct gaatttcttt tacactggcc
     1741 gtatcaagat agaacttcat agccgtgtcc tttcatgtct gcgcactcct acttacattg
     1801 taaaaagaat aacctgatga tgcaaacggg ctagtctcac ccgcccaccc cgcggcgccg
     1861 agacgccgcg tttgcgccca ccgccactcc tgtttgacac ccctatgacc cgcggttaga
     1921 atccctcgcg cgccgttctt gtagaaggac gcaacgaacc ggatgattgc gaggagagac
     1981 ctccatgacg cgacgatggc tgctggcgct gatcgggtgc ctgttcctgt ccgcctgcgg
     2041 cggaaataat ccctaccttg aacaatcctt gaaaccagcg gagctgcagg ggaaagacaa
     2101 atcctggttc ctcaagaatt ggggcgagcc gagcggcaag gcgcctcgct tcttcggcgg
     2161 cgaaacatgg acctacttcc gcatcgccgg cggaaaatcc ggtccgccac tcttcaattt
     2221 ctcaccaaat cagtgcgaga tcaccttgaa gttcgacaaa gaagaaaaac tgtcggacta
     2281 tagttattcc ggctgctgat tccccactgc gccggctacc cggtcatctg cctttgaaag
     2341 gttgttgcgc agccctggct gcaaaaacaa taggtctgac cgccaatgac ttcggtcacc
     2401 gccgaccctc tgggaacata tacacggcac acgggatcct gcaccatctg atctttgggt
     2461 ttcggcagac gttcctgggt tcgagtccca aactcccgga aggcgcttcg gagcaggaag
     2521 tacaggatga tcagcaggat aagaatgatg atgattctat acatagtaga ggtgcgagtc
     2581 ggctgccaca atcctatggg agcgtcgagg gtgtgtcaac ggccgatgtg aacgtacacg
     2641 aacggtcagt tcgaactcct atagctccaa tggtctagct tctaccccaa caaccaggcc
     2701 ttaattccct cacgacccca gtacttctga tggataggca cccttcggtc gcagtgttag
     2761 agtgaccgca tgaagacctg tgacaagtgt cgcggaacaa tgttgccgga acgggcg
//
LOCUS       DASSCQ010000149         4322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_469757, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000149 DASSCQ010000000
VERSION     DASSCQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469757"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..979)
                     /gene="modA"
                     /locus_tag="VFH05_10475"
     CDS             complement(152..979)
                     /gene="modA"
                     /locus_tag="VFH05_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6678687.1"
                     /translation="MWPRVLRCGLTLIVWMVTASAYATETLMIATSPSVAPAVEALGR
                     QFETTHPDVRVRLYLDSGLDLRRTIAGMENNPRGQYFIGSGPIHLVAPGGDELLTRLE
                     QKYYVLPGTTRPYAEVPLVMVVPEALVDAPASFESLTQNKTLRIAVGDPALTMLGQRT
                     MQLLMTLGVWEAVQPRLDRASDVRSVLDHVLNGEADVGIVFGPDAVQESQRIRVVAVS
                     ERDIIPPVIHSMAMERYCPNRALCKEFLSFIQSDEAQRELQRLGYTSPSQSQPTRNE"
     gene            complement(1014..2159)
                     /locus_tag="VFH05_10480"
     CDS             complement(1014..2159)
                     /locus_tag="VFH05_10480"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6678688.1"
                     /translation="MSEAPPIQNRLREVRIASRLSQGELALQAGMTRQAVSAIESDLY
                     LPSTAVALRLAGVLSCRVEDLFILAASEEVLEGRYLGAHEKQPTTPLRVKVTKVGKNI
                     IVRPVADLGEVLAYTVPADGYLLGTTTVKSGITGRVRLSRDRQAIDQEISVAGCDPAI
                     FLAGEHLQRHKDRTSVVGWSMGSMAALGALQRNEVHVAGLHLFDPLSGESNLPFLKRA
                     LKGSAYEVVTFATWEEGFLVRPGNPKTIRQAADLADEAVTLVNREEGSGARLLLDQRL
                     RAAGVDQARVRGYENTARSHFEVARTIAGRQADVGIGIRSAAQSFGLDFVPLQSARYD
                     LVVAKSILQSHPTLGHLFDAISSRRFRDEIEALGGYDTRDTGKLHAL"
     gene            2638..4272
                     /locus_tag="VFH05_10485"
     CDS             2638..4272
                     /locus_tag="VFH05_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678689.1"
                     /translation="MRISGAFVLVIAGMMLLNHAGPAAAVEEGQFVKKEGKWEYYSGE
                     DPGLKYLYLKGLITQEEYDKGLRVIETKERLSRPNFSVDVNNGLNVRVGDRFLLKLRL
                     LAQARYSYATYNSAWGTVGDSRNPEILGGQVEYRAVRYQSNSSSFTVPRTRLQFLGYA
                     WDPDFRYNVSWAFDQTAWDQEGGSGRARLLDAYIASWHIPWATVQLGQQRVWFNRSQI
                     SSIATSTFADNMRVQNAFAANLISNRDVGISILSDEDQYRFNYALGIWNGAGPNLARD
                     GTSVSQAVLPPTGAGGPPAGTTRTYNYNTRFLNGEMMYTARLLFKIAGNPGYGQGDIL
                     NSRTPQAAIAFGYAYNPGQNYLSSIRSDIVDRAYRQGVAKNGNGRLLAGGVYDFQTYE
                     VDLIAKYHGWSLQSEGFYRHQRVRHTDSGTVPFDLSTIPAPQFLGPPVELGQAWGWYV
                     QAGKYLIPRKLEVAVRYGMMDPSTKQQDDLTKELGVAINYSFDGTYNNRLVVDYSNIT
                     MGSGGRAPDRFPFESQPGFGRDLIENRINVQYQFYF"
BASE COUNT         1002 a         1236 c         1157 g          927 t
ORIGIN      
        1 catgaatgac cttggttgta tacggaagtc gcgcgcatcc tcccgccatg atttccatga
       61 acaccacaaa gataagacca tagggtgaca agcacgccat ccggctccac atcaacgcct
      121 ccctcctctc aaatacgtgc agcatttgta gtcattcgtt ccttgtgggc tgactctgtg
      181 acggcgatgt gtaccccagt cgctggagtt ccctctgggc ttcatcagat tgaatgaagc
      241 tgagaaactc tttgcagagg gctcgattgg gacaataccg ttccatcgcc attgaatgga
      301 tcactggagg aatgatgtcg cgttcggaaa cggccacgac acgaatgcgt tggctctcct
      361 gaaccgcgtc cggaccaaag acaattccga catctgcctc accattgagc acatgatcca
      421 ataccgaacg gacgtcgctt gctcgatcca atcggggttg caccgcttcc cagactccca
      481 acgtcatcag cagttgcatc gttcgttgcc cgagcatggt cagcgctgga tcaccaactg
      541 caatccgtaa ggttttgttt tgcgtgagtg attcaaacga cgccggcgca tcaacaagcg
      601 cctctggcac aaccatgact aacggcactt ccgcataagg tcgcgtggta ccgggaagga
      661 catagtattt ttgctccagg cgagtaagaa gttcgtctcc acccggtgcc acgagatgta
      721 tcggcccgga tccgatgaag tattggcctc tcggattgtt ttccattcca gcgatggtcc
      781 ggcgaaggtc gagcccagaa tcgaggtaca gacgcacgcg aacgtccgga tgggttgttt
      841 caaattggcg tcccaatgct tctacggccg gagccacact tggtgaagtt gcgatcatca
      901 acgtctcggt cgcataggca gaggccgtca ccatccacac tatgagagtc agaccacagc
      961 gcagcacgcg cggccacatg acgctctcat tcgtcggatc aagtcgttgt cggtcacagc
     1021 gcatgaagct ttcccgtgtc tcgtgtgtcg tacccgccca acgcttcgat ctcatcgcga
     1081 aaccgccggc tgctgatggc gtcaaataaa tggccgagag tcggatggct ttggagaatg
     1141 gatttcgcca cgacgaggtc gtagcgggcc gattggagcg gcacgaaatc cagaccgaag
     1201 gactgagcgg ccgatcgaat gccgatgccg acgtcggcct gtcggccggc tatcgtcctc
     1261 gcgacctcga aatgggagcg ggccgtgttt tcatatccgc gcaccctggc ctgatccacg
     1321 cctgcagccc gcaaacgctg atcgagcaag agccgggcac cagagccctc ctctcggttg
     1381 acgagagtca ccgcttcgtc ggccaaatcg gccgcctggc gaatcgtctt tggattcccg
     1441 ggccggacca gaaagccttc ctcccaggtt gcaaatgtca ccacctcgta ggccgaccct
     1501 ttgagcgcgc gcttgaggaa cggcagattc gattcgccgc tgagtggatc aaacagatgc
     1561 aagccggcca catgcacctc attccgctgc agggctccga gcgcagccat gcttcccatc
     1621 gaccagccga caacggaagt tcgatcctta tggcgctgca ggtgttctcc agcaagaaaa
     1681 atggctggat cgcagccggc aaccgagatc tcttgatcaa tcgcctgacg atcccgtgaa
     1741 agccggacac gccccgtgat tccagacttg accgtcgtcg tccccagcaa atacccatca
     1801 gccggcacgg tgtaggccag gacttctccc agatcggcga cggggcgaac gatgatgttc
     1861 tttcccacct tcgtcacttt cacacgcaga ggagtggtcg gttgcttctc atgtgcgccg
     1921 agatatctgc cctccagcac ctcctccgag gcagcaagga tgaatagatc ttcgacgcga
     1981 caagagagaa cacctgcgag ccgcaaggcg acggctgtgg atggaagata gaggtcggat
     2041 tcgatcgcag aaactgcctg tcgggtcatt ccagcttgca gcgccagctc gccttgcgaa
     2101 agccgactgg caatacggac ttccctcagt cggttctgaa taggtggcgc ttcggacatg
     2161 acggttctag gtagcactta tacttgcctt acgtcaagta tacttgccac agctctaccg
     2221 tttgctttgc tcacatagcc agccgatggg tacataaatt atttgttgac aaatatgatc
     2281 atattaatta ttaatacatc attactaact ctaccgagat actggtaatt caattaacat
     2341 ccaggcaata tgaggcccag catgcacaca acggatcaac attaccgggt agacctggcc
     2401 attcaggagg cgttgaggga tctccatttc ggatccgttg aaatcactgt ccacgattcg
     2461 aaggtagttc tgatcgagcg tagagaaaag atgcgcctgg actcacctgt gccccattac
     2521 atgaaaaaag catgatgaga cgattctagt cctgaggagg gacgactact tcttgtaatc
     2581 acatcgtcat gaactgaccg gaccaccgga agttcctatg tcacggagga cctcgacatg
     2641 agaatttccg gtgcgttcgt tcttgtcatc gcaggcatga tgctgttgaa ccatgccgga
     2701 cctgcagcgg ctgttgaaga aggacagttc gtcaagaaag agggaaagtg ggagtactac
     2761 tcgggcgaag atcctggcct caagtatctg tatctcaaag gccttattac gcaggaagaa
     2821 tatgacaagg gtctgcgagt cattgaaact aaagagcggc tctctcgacc gaatttcagc
     2881 gttgacgtca acaacggcct caacgtccga gttggagaca ggttcttgct caagcttcgc
     2941 ctccttgctc aggcccgtta cagttatgcc acgtacaatt cggcctgggg aaccgtcggg
     3001 gactcccgaa atcccgaaat cctcggtgga caggtcgaat atcgcgccgt ccggtatcag
     3061 agtaattcca gcagcttcac ggtgccgcga acgcggcttc aatttctcgg atacgcatgg
     3121 gatccggatt tccggtacaa cgtttcctgg gcgtttgatc agaccgcctg ggatcaagaa
     3181 ggcggcagcg gaagagcgcg gctcctcgat gcctacatcg cctcctggca tatcccatgg
     3241 gcgaccgttc agctcgggca acagcgcgtg tggttcaacc gttcccagat cagttcgatc
     3301 gcgacctcga cgttcgcgga caatatgaga gtgcaaaacg cgtttgccgc aaacctcatc
     3361 agcaaccggg acgtgggcat cagcattctc agtgatgagg accagtatcg tttcaattat
     3421 gcattgggca tttggaatgg agccggacct aatcttgcca gggatgggac atcagtcagc
     3481 caggccgtgc tgcccccaac cggtgccggc ggtccgcccg cgggaacgac acgaacgtac
     3541 aactataata cgagatttct gaacggtgag atgatgtaca ccgccagact gctgttcaag
     3601 atcgcaggca accccggcta cggccaggga gacattctga attcacggac acctcaagcc
     3661 gcgatcgcgt tcggctatgc gtataatccc ggtcaaaact atttgagttc tattcggtca
     3721 gacattgtcg accgcgccta ccgccaaggg gtcgcaaaaa acggcaacgg caggctcctc
     3781 gccggcggcg tctacgattt ccaaacctat gaagtggatc ttatcgcgaa gtatcatggg
     3841 tggtcccttc aatcggaggg cttctatcgg catcagcggg tcagacatac cgatagcgga
     3901 accgtgccgt ttgatctcag cacgatcccc gctccgcagt ttctcggccc tcccgtagag
     3961 cttggccagg cgtggggatg gtatgtccag gcgggaaaat atctcatccc tcgaaaactc
     4021 gaggtggcgg tcagatatgg catgatggat ccgtccacca agcagcagga cgacctgacc
     4081 aaagagctcg gcgtcgcgat caactacagc tttgacggca cctataacaa ccgtcttgtc
     4141 gtcgattact ccaatatcac gatgggaagc ggcggccggg ctccggatcg attcccgttt
     4201 gaaagccagc ccggctttgg tcgcgacttg atcgagaatc gcatcaacgt gcagtaccag
     4261 ttttacttct aacgatcaca tggttacgcc acaccgacat gaaatgagga ctcccatgac
     4321 ag
//
LOCUS       DASSCQ010000150        11849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_947231, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000150 DASSCQ010000000
VERSION     DASSCQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947231"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFH05_10490"
     CDS             <1..315
                     /locus_tag="VFH05_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET6678690.1"
                     /translation="VYDPGSDKWTKAPDLPTARSGITAAEIGGRVYVFGGEGANGTFH
                     ENEAYDPAREAWQVMAPMPTARHGLGSSVINGHIHVISGGPTPGGSFSNLNEVFSPPG
                     SR"
     gene            complement(331..885)
                     /locus_tag="VFH05_10495"
     CDS             complement(331..885)
                     /locus_tag="VFH05_10495"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET6678691.1"
                     /translation="MKLLSIQVGRPRKVTWRRRTVTTGIYKAPVEGRIMLRPHNLEGD
                     VQADLRVHGGWDKAVYVYPSEHYAFWRAELPGMQLPYGMFGENFTTEGLNESIVYIGD
                     RFSIGSAVVEVTQPRMPCYKLGVRFGRLDMPQRFHVSGRCGFYLAVVAEGDVGAGDAW
                     ELIERRVDTMSVLDRYRSTFEEAD"
     gene            complement(948..1844)
                     /locus_tag="VFH05_10500"
     CDS             complement(948..1844)
                     /locus_tag="VFH05_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6678692.1"
                     /translation="MTDHIPSIRYSKQDVDGINIFYREAGSPTSPAVLLLHGFPSSSH
                     MFRGLIPLLAQHFHVIAPDLPGFGFSDAPSRAHFAYTFDHLTDVMERFTQAIGLARYA
                     LYIFDYGAPVGLRLALRHPERITAIVTQNGIAYEEGLSAGWNPIQRYWAEPTQENRNA
                     LKTFLSPDATKWQYTHGTDESHIAPESYTLDSALLARPGNEDIQLDLFLDYGNNVTLY
                     PRFQQYFREHQPPLLAVWGKEDPFFLPAGAHAFMRDNPNARVVFFDTGHFALETHGAE
                     IGAKIVEFLRALEPVGVAVSGQ"
     gene            1987..2595
                     /locus_tag="VFH05_10505"
     CDS             1987..2595
                     /locus_tag="VFH05_10505"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HET6678693.1"
                     /translation="MRSKPQFLFVGNHPCLDFINTELVINGQPTDLLSSFQDLLAWLL
                     QGGLVTGEEAKRIERVGKRQEKDILKQAKAFRTDMHDMVRRLSDGEPVPQAALETINH
                     LLRHRIGYSQISRRSGNRFERNYQSELEDVNQLLGLLGKVASDLLVTCNLSLVKKCQN
                     PACVLFFYDTTKNHARHWCSMSLCGNRSKVAAHYHRHRKGRP"
     gene            2592..3215
                     /locus_tag="VFH05_10510"
     CDS             2592..3215
                     /locus_tag="VFH05_10510"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HET6678694.1"
                     /translation="MMATEPTVVGVDVGGTQKGFHAVALRHNQIVAKLTTCSALDVAT
                     LCREQGAAAVGIDAPCQWSLTGRARPCERELAGLGMSVFSTPSQAVGQVHPFYRWMVN
                     GAELFQLLVPDYRLYDGRSAPLDPLCFETFPQAIACSLAGRNLSAKNKRADRRRLLVQ
                     AGISSEALPTIDDIDAALCALSAQHVLAGRFKAYGDAAEGFILLPAR"
     gene            3734..4207
                     /locus_tag="VFH05_10515"
     CDS             3734..4207
                     /locus_tag="VFH05_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678695.1"
                     /translation="MACTVYLPPMLFMVGPAAIVTQHPLILIVLHSVIGFGMSWSLHR
                     MGHCLLMALPKTPQKIYLCGSLCLPVQLTLSQFICMMELLVAAGVFCGIVAKRLLAED
                     LVSSLLLFGFGIVLYFTPVYLTRLWTQRYYPIMTLVRPTEAVIRKSFPGLRSLFQ"
     gene            4524..4988
                     /locus_tag="VFH05_10520"
     CDS             4524..4988
                     /locus_tag="VFH05_10520"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HET6678696.1"
                     /translation="MKSLIGTAALALFFAPTLLGPVWAALGDTSSSVERDRVMMKGQR
                     HSRTGTGYSIDTITVAGIQVKEYVSPDGIVFAVVWSGTGTPDLHLLLGEYFEEYREGV
                     DAARSRRPKIRKPFQLKSERLVVERGGHSRSAWGRAYLPAALPAGVTPEDIQ"
     gene            4985..6148
                     /locus_tag="VFH05_10525"
     CDS             4985..6148
                     /locus_tag="VFH05_10525"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 domain-containing protein"
                     /protein_id="HET6678697.1"
                     /translation="MRLVTAVGVVVCLLAGCNGDDDNGPSSAGNVVPISVGESAVCRT
                     INQLCAVITICQPGTSNCQTVSDVLVDNGSVGLRVFGSVLSTPLMQTVDAQGRPVGEC
                     TSFADGGTTWGPVQVADVILGGAPAVRVPIQVIQSTFGGQSSSSNPCNDEVDIDPEAA
                     SFNGILGIGIFRQDCGPVCAANSNNTLYFSCAGGSCTGVAVPLADQVQQPVWLLPSGN
                     NGMVITLPNVPGMGAPSVSGSLILGIGTADNNTPPAGIPVMTTDDNGFVTTVYKGRTF
                     SQSIIDSGSNGLFFPDASLQQCATPLDPFYCPPSTVNLSGTVIGANGRQVVVPFQVAN
                     TESLVQTGNAAFSNLGGDFSIFDWGLPFFFGRTVFIGIEGQSSSLGTGPYFAF"
     gene            complement(6432..6659)
                     /locus_tag="VFH05_10530"
     CDS             complement(6432..6659)
                     /locus_tag="VFH05_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678698.1"
                     /translation="MMEETIELLFGNRFFQLPFLEAMSYIAAALLIGAIFVGLTRRLM
                     GSSAQPVESTVYRQPSYSQPYEESYEWRKAA"
     gene            complement(6896..7807)
                     /locus_tag="VFH05_10535"
     CDS             complement(6896..7807)
                     /locus_tag="VFH05_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET6678699.1"
                     /translation="MALSPLVRFGTSTWTYEGWRGEVYKQPYPKGRFKQDCLAEYARY
                     EHEGEPLFRTVGFDFTFYGPPTAKQLEHYASLLPPGFEACAKVWEEITIPLFPPGLRY
                     RSKAGPNPRFLDADYFLDQVLTPFTQGFEPHTGPFIFEFQRTGLEPDTFLPKLDAFFR
                     RLPTRFKYAVEVRNPAVIGPVYRDLLKAHGVSHVYNYLYGMPTLEQQHEKLGHEFTAP
                     FTLLRLLTPRDKKYHEAVKAYEPYDKLVRPLPDMRASSVRLVRQAIEERRRAYVLVNN
                     RSEGHAPGTVKAIRDILIQQEKTSENT"
     gene            complement(7938..8912)
                     /locus_tag="VFH05_10540"
     CDS             complement(7938..8912)
                     /locus_tag="VFH05_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6678700.1"
                     /translation="MAHDVTLHPGLSPRLTQWSVPTLLLLSAYLTGDCSVQAIAESQN
                     GLRPLRVVTFNLLHDGAGSGFLDGDTHLEERLEMTIRELKTLDPDIIAVQEASDSRRH
                     GNVPERLAKALGLHVVFEPATEHVFGLWPLDSIVVGMMGFKEGSAILSRFPIAASHVY
                     DLPRCKKWLEPRILLQAELSTLRGPLQVFSTHTGRGDECQMERVGEIVRARLGGGPSL
                     LMGDFNTPETSQVLMTLRNEAGFVDAYRLANPDAAGPTVWQRIKSKEPTVSRRVDFIL
                     LLNGRQQVASVRSSRLVLDHPGQLPDGTTLWPSDHYGVLAEVDIHSVP"
     gene            8904..9488
                     /locus_tag="VFH05_10545"
     CDS             8904..9488
                     /locus_tag="VFH05_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678701.1"
                     /translation="MRHPVILLFSACLVGGWLSIVPCMAAHGDADAHGMKVGQWTGGA
                     GVGFLGNTPDGVLEFGLDGHVEYVIAERFSIGPMVQYAGTGNDFLFGLSAQVKYWWDI
                     PGTQGATKLVLQGGLGFVRAGITDTDSGTGNTYGSFLIPIGVGVDHAMRPGMVLTADL
                     LLNFTSVGETVRAGGREFDLHTNIMPAFYLGVRF"
     gene            complement(9522..10247)
                     /locus_tag="VFH05_10550"
     CDS             complement(9522..10247)
                     /locus_tag="VFH05_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET6678702.1"
                     /translation="MSRDRHVPIPESSSRPSARGKFIVAAVFLAVICAFYFFDLMAYL
                     SLDMLKANRDKFLSFTEEHYAQAVLLFILVYIAQTAFSLPGATLLTLMGGFLFGTLLA
                     ALYVNIGATTGATLAFLAARYLFRDWVEQRVGDRLAAFQAGFARNAFLYLLTLRLVPI
                     FPFFVVNLLSGLTRINVGTYVAATALGIIPGSLAYTFAGRQLGTINSLAELASPRLLL
                     AFTVLGLLFLMPVVYRKFVRPQP"
     gene            10479..11252
                     /locus_tag="VFH05_10555"
     CDS             10479..11252
                     /locus_tag="VFH05_10555"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET6678703.1"
                     /translation="MKLSSPASDSKKARVPMVVVSLLVALLVVPVIVHAHDETADKPS
                     ITVSGHGHIALAPDTAFVTLGMETVGKSVGEAQRQNRQAMNKVLERLRGLQIEQERIQ
                     TASFTVSPQYKPPPKRTADAPPAPPEIIGYIVSTNLTVEVRNLEKVGAVIEESLAAGA
                     NRFQALHWGLLDEQQAKLSALKQAAVKAREKATALSESLKVKLIRLMSATEDSHLVRP
                     LPKLARSMMMEGGSEETPVSAGEIKIDAIVTLNYEVGQD"
BASE COUNT         2475 a         3286 c         3488 g         2600 t
ORIGIN      
        1 gtgtacgatc ccggctcgga caagtggact aaggcgccgg accttccaac ggccaggagc
       61 gggatcactg ccgctgaaat cggagggcgt gtctatgtgt tcggcggcga aggcgcgaac
      121 ggaaccttcc atgaaaacga agcctacgat ccggctcgcg aggcctggca ggtgatggcg
      181 ccaatgccaa cagcacgtca cggccttggg tcgagcgtca tcaatggcca catccatgtg
      241 attagcggcg ggccgacgcc tggcggctca ttcagtaatc tcaacgaagt tttctcacca
      301 ccggggagcc ggtgagacca ttgccgccgc tcagtccgct tcctcaaaag tactacgata
      361 cctgtcgagc acggacatcg tgtccaccct tcgctcgatg agttcccaag catctcccgc
      421 cccgacgtca ccttccgcca ccacggccaa ataaaagccg caccggccgc tcacatgaaa
      481 ccgttgtggc atgtctaggc gtccgaaccg cacgccgagt ttgtagcagg gcatgcgggg
      541 ctgcgtcact tcgaccaccg ccgagccgat gctgaaccga tcgccgatat agacaatcga
      601 ttcgttcaat ccctcggtcg tgaaattctc accaaacatg ccgtacggca actgcatgcc
      661 gggaagctca gcgcgccaga acgcataatg ctcggatgga tagacgtaga ccgccttgtc
      721 ccatcccccg tgcactctca gatcggcttg cacgtcgccc tctagattgt gcgggcgcag
      781 catgatgcgc ccctctaccg gggccttgta gatgccggtc gtcacagttc tgcgtcgcca
      841 tgttaccttg cgcggacggc ccacctgaat ggaaagcagt ttcatcgtga cctcccaata
      901 tagagccggc ccggcggtca gtgctccgtc gcgatataag tcctcactca ctgcccactg
      961 actgcaactc ctaccggctc cagtgcgcgc agaaactcca cgatcttcgc accgatttcc
     1021 gcgccgtgcg tttcgagcgc gaaatggccg gtgtcgaaga agacgactcg cgcattcgga
     1081 ttatcccgca tgaacgcgtg cgcccctgcc gggagaaaga atggatcttc tttgccccag
     1141 acggccagca gtggcggctg atgctcacgg aagtactgtt gaaagcgcgg atagagcgtc
     1201 acgttgttgc cataatcgag aaacagatcg agttgaatgt cctcgttacc ggggcgtgcc
     1261 aacagcgcgc tgtcgagcgt ataggattcg ggagcgatgt gcgattcatc cgtgccatgc
     1321 gtgtattgcc acttggttgc gtcgggcgag agaaaggttt tcaacgcgtt gcggttttct
     1381 tgagtgggct cggcccaata ccgctgaatc ggattccagc cggcgctgag accttcctcg
     1441 tacgcgatcc cgttttgggt cacgatggcc gtgatccgct cgggatgacg gagtgccagt
     1501 cgcaggccga caggagcacc gtagtcgaag atataaagcg catagcgagc gaggccgatg
     1561 gcttgcgtaa agcgttccat cacatcagtc agatgatcga aggtataagc aaagtgtgct
     1621 cggctggggg catccgagaa gccgaaccca ggcagatcgg gagcgatgac gtgaaagtgc
     1681 tgcgcaagca gtggaatcag cccgcggaac atgtgcgatg acgagggaaa gccatgcagt
     1741 aagaggacgg cgggagatgt cggtgagccg gcttcccgat aaaaaatgtt gattccatca
     1801 acgtcttgtt tggagtagcg gattgatggg atgtgatcgg tcatacgaac tcctttggta
     1861 tgtgcgccgg agttgccggc gtcgggtgat gaattatgcg aaatagcctg acgcacacta
     1921 actatctaaa cattagattg acggttatac caatattcgg ctaacctgtc aagcatggat
     1981 tcaccggtgc gatcaaagcc tcaatttctt ttcgtcggca accacccgtg tttggatttc
     2041 atcaatacgg agttggtgat aaacggacag ccgacggact tgttatcgtc gtttcaggac
     2101 ttgctcgcct ggcttcttca gggcggtctc gtgactggag aggaggccaa aaggatcgag
     2161 cgggtaggga agcgccagga gaaggacatc ttgaaacagg cgaaggcgtt tcgtacggac
     2221 atgcatgaca tggtcagacg tctctcggac ggcgagcccg ttccgcaagc ggcgctcgag
     2281 accatcaacc atctgctgcg ccatcggatt gggtattctc agatcagccg gcgaagcggc
     2341 aacaggttcg agcggaacta ccagtcggag ctcgaggacg tcaatcaact gctcggattg
     2401 ctggggaagg tggcgagcga tctcctggtc acgtgcaatc tctcattggt caagaaatgt
     2461 caaaacccgg cgtgcgtgct ttttttctac gatacgacca aaaaccatgc acggcactgg
     2521 tgcagcatga gcctttgcgg taatcgcagt aaggttgccg cccactatca tcgacacagg
     2581 aaaggcaggc catgatggcg acagagccca cagtcgtcgg cgtcgatgtg ggcggcaccc
     2641 agaaaggctt tcatgccgtt gccctgcggc acaatcagat tgtggccaag ctcacgactt
     2701 gcagcgcctt ggatgtggcg accttgtgtc gcgaacaagg cgcggcagcc gttggcatcg
     2761 atgcgccatg ccagtggagt ctcaccggac gggcaaggcc ttgtgagcga gagctagccg
     2821 gtctcggcat gtccgtcttc tctacgccga gccaggcggt cgggcaagtc catccctttt
     2881 acaggtggat ggtcaacggc gctgaactct tccagttgct cgtgccggac taccggttgt
     2941 acgacggtcg atctgcgccg ctcgatcccc tgtgttttga aacgttccct caagccatcg
     3001 cctgctcatt ggccggtagg aacctttcgg cgaagaataa acgggcggat cggcgtcggc
     3061 tgttagtgca agcaggcata tcgagcgaag ctcttccgac catcgacgat attgatgctg
     3121 cgctatgcgc tttgagcgct cagcatgttc tcgctggccg ctttaaggcc tatggtgacg
     3181 ccgccgaagg cttcatcctc ttgcctgccc gctagttcct gtcaaaaagc tattcaacga
     3241 ttcccaagcg agcgctgcgc gtgagagagc atgcattgcc agctcgtctg cactcgtgtg
     3301 ccaccgcccg taaggatgta tggccttacg gcgacttgcg atcggtcgta tgacgctgcc
     3361 gtgcgctcgc gttagcgatg gcgagcattg tttgaagttc cgttcggatc gaagcctcgg
     3421 acggaacaag ttgcggagcc aagcgcggca gcgtcatgcg gttttatcca tcgcaggagc
     3481 cggcaggcca tcatccgcaa acctcttgac ttcgttttgc gcttccatta caaaggccca
     3541 cgttcagtgg cgtgagcatg caggaacagg aacccggctg cacccctaat gcactccgcc
     3601 tcgatcacat cctctcatag gtaaccggaa cgattcgact ttcgctcgta tggaatccaa
     3661 cgagacgcct cggactgaca tcttcacaca aggctgccgt gacctcgttc ggaggttcac
     3721 atacgaggcg attgtggctt gtactgtcta tcttccgcca atgctcttca tggtggggcc
     3781 cgcggcaatc gtcactcagc atccgttgat cctcatcgtc ctccatagcg tcattggttt
     3841 tgggatgagc tggagtctcc accgtatggg acactgcttg ctcatggcgc tgccgaagac
     3901 accgcagaag atctatctct gtgggtcgct gtgcctgccg gtgcagctca ctctcagcca
     3961 gttcatttgc atgatggagc ttctcgtggc ggcgggcgtc ttttgcggga tcgtcgccaa
     4021 gcggctgctc gccgaggatc tcgtgagcag cctcttgctg ttcgggttcg gcattgtgct
     4081 gtacttcaca ccggtctacc tgacgaggct ctggacccag cgatactatc ccatcatgac
     4141 gctcgtgcgg ccgacagaag ctgtcatcag aaagtcgttc ccgggcctgc gctccttgtt
     4201 ccagtaagca cgacaatttc cctccctcta tgcccggaaa tccgattcga tggacgagac
     4261 tttgacgatc gtcttgacgt tttttggtgc cagggagcac ggtctttccc ctcttccatg
     4321 gggtggacaa ggtcgctcca caatcagcat gatggtagaa tcttccgctg ccccacctag
     4381 gcaaaatatt gaacctgttc ccgcgtaact tggatgataa ccgcgggcaa ggggccaaac
     4441 aaggaagagc tagggaacct atcaacgggg cattgtcctt aatgcgtctt agctatcgta
     4501 agccttaccg tgaaagtccc cggatgaaat ctttgattgg cacagcagca cttgccctgt
     4561 tctttgcgcc gacactgctt ggtccggtct gggcggcgtt aggagacacg tcgtcctccg
     4621 tggagcgcga ccgcgttatg atgaaggggc agcggcacag ccggacgggg acggggtatt
     4681 cgatcgatac gatcacagtt gccgggatac aggtcaagga atacgtctcg cccgacggga
     4741 ttgtgttcgc cgtcgtctgg tcagggacgg gaacgccgga cctccacctt ctgctcggtg
     4801 agtatttcga ggagtaccgc gaaggagtcg atgccgctcg aagccgccgg ccgaaaatac
     4861 ggaagccctt ccaattgaag tccgagcggc tggtcgtgga gcgcggcggg cattcccgtt
     4921 cagcatgggg acgtgcctat ctaccggctg ccttacctgc cggggtcaca ccagaggata
     4981 tccaatgaga ctggtaacgg ctgtcggtgt tgtcgtgtgc cttcttgccg gttgtaacgg
     5041 agacgatgac aatggaccgt catcggcggg caatgtggtg cccatatccg ttggggaatc
     5101 ggccgtctgt cgcacgatca atcagctctg cgctgtcatc acgatttgcc agcctggcac
     5161 ctccaattgc caaaccgttt cggacgtgct tgttgataat ggatcggtgg gcctgcgggt
     5221 gtttggctcg gtattgtcca cgccgcttat gcagacggtc gatgcccagg gccgcccggt
     5281 cggcgaatgc acatcttttg cggacggcgg aacgacatgg ggtcccgtgc aggtcgccga
     5341 tgtgatattg ggtggtgcgc cggccgttcg cgttcccatt caagtgattc aatcgacctt
     5401 cggcgggcaa tcctcgtcga gcaatccctg caacgacgaa gtggatatcg acccggaggc
     5461 ggcgagcttt aatggcattc tcggcattgg catctttcgg caggattgtg gaccggtctg
     5521 tgcagccaac agcaataata ccttgtactt ttcctgtgct ggcggctcct gcaccggtgt
     5581 cgcggtgccg ttagctgatc aagtgcaaca acccgtgtgg ttgctcccct ctggtaacaa
     5641 cggcatggtc atcactttac ccaatgtgcc tggcatggga gcgccgtctg tctccggatc
     5701 tctgattctc ggaatcggga cagcagacaa caataccccg ccagcgggca tcccggtgat
     5761 gaccacggat gacaatgggt ttgtcacgac ggtttacaaa ggcagaacat tcagccaaag
     5821 catcatcgac tctggctcta acggcttgtt ttttccggac gcgagcctcc agcagtgcgc
     5881 gactccgttg gacccattct attgcccgcc aagtacggtc aatctctccg ggacggtaat
     5941 cggagcgaac ggccgccagg tcgtcgtgcc ttttcaggtc gccaacacag aaagcttggt
     6001 gcagacaggc aacgcggctt tcagcaatct cgggggagac ttttcgatct ttgattgggg
     6061 gctgccgttt tttttcggcc gtacggtatt catcggaatc gaaggccaat cgagcagcct
     6121 cgggacagga ccctactttg ccttttgaga ctagtgcgtt tctggcagag cgctggtcaa
     6181 tttcctagga tccgtttctc cgccactgcg gtaaggtgaa attactttag aaaacggcag
     6241 tggaaacatt gccgcatctc cgaggtagga gcccgctttc cgaaagggag cgggctcttt
     6301 tttttcaggg cactagaagt ctagcaaggc cgtgatgtgt ctgaggaatg cgcggtgtat
     6361 tggtctctca tggccgtcct tcatagcacc gctcgcgtca ggcggacacg agagaccaac
     6421 agccttcctg tttaagcggc ttttcgccac tcgtaggatt cttcgtaagg ctggctatac
     6481 gagggttgac gatacaccgt gctctcgaca ggctgcgccg aggaccccat gagtcgcctt
     6541 gtcagaccga cgaagatggc tcctatcagc agggcggcgg cgatatatga catcgcctcc
     6601 aaaaacggaa gttgaaagaa acgattgcca aataacaatt caatggtctc ttccatcatg
     6661 aacctccttg tgagctggtt ttgtcccgat gctcgagacg ttgatactga gaatgaggta
     6721 cccgccaggg ggcggctggc actctaggac ggggcggcaa cctcacaggt tgttttgcaa
     6781 tgcagatgcc ttgtgtaagt gggcaaagtt gcgtgaagag gggtatgcgt cggttgaaac
     6841 gctttgtcct gcgacggaag aaatgtctgg tgcccgggaa atccccagac tcgaattagg
     6901 tattctccga cgtcttttct tgctggatta atatgtcgcg gatggccttg actgtgcctg
     6961 gcgcatggcc ttcgctgcgg ttgttgacca gcacataggc acgccggcgc tcttctatgg
     7021 cttgcctcac caggcggacg cttgatgcgc gcatgtcggg aagggggcgg acgagtttgt
     7081 cgtacggttc atacgctttc accgcctcgt gatacttttt atcacgcgga gtcagcagcc
     7141 gtagcagggt gaagggcgcg gtgaattcgt gtccgagctt ctcgtgttgt tgttcaagcg
     7201 tcggcattcc atataaatag ttgtacacgt gactgacgcc atgtgccttg agaaggtctc
     7261 gatagacagg gccaattaca gcgggattgc gaacttctac cgcgtacttg aagcgagtgg
     7321 gcaggcgtcg gaaaaaggca tctaacttcg gcaaaaatgt gtcgggctcg aggccggttc
     7381 gttggaactc gaagataaac ggtcccgtat ggggttcgaa cccctgcgtg aagggagtaa
     7441 ggacctgatc gagaaagtag tcagcatcga gaaatcgtgg attcggcccg gccttactgc
     7501 ggtagcgaag cccgggcgga aacagcggga ttgtgatctc ttcccacact ttggcacagg
     7561 cttcgaagcc gggcggcaat agtgaggcat agtgctcgag ctgtttggcc gtcggcggac
     7621 cgtagaacgt aaaatcgaaa ccgactgtcc gaaacagtgg ttccccttcg tgctcatagc
     7681 gcgcgtattc ggccagacag tcttgcttga accggccttt cggatacggc tgtttgtaga
     7741 cctcacccct ccagccctca taggtccaag tcgaggtgcc gaaccgtacc aatggagaaa
     7801 gcgccatgtg gactttttac tctcttttcc tctcttcatg agagggtacc cgattaagat
     7861 tactagagac aaagcgtgtg ctgcttttta ccgtaccaag gcacggcaaa ataaagctat
     7921 ctccttccgg caccagccta aggaaccgag tggatgtcca cctcggcaag gacgccgtaa
     7981 tggtcggacg gccagagcgt cgtcccgtcc ggcagctggc caggatgatc gagcacgagc
     8041 cggctggatc gtactgaggc gacctgctgt cggccattca atagcagaat gaaatcgacg
     8101 cgacgagaga ccgttggttc ttttgattta atccgctgcc acaccgttgg tcccgctgcg
     8161 tcgggattgg cgagacggta tgcatcgacg aatcctgcct cgtttctgag tgtcatgaga
     8221 acctgagagg tttccggtgt gttgaagtct cccatgagca gcgagggacc gccgccaaga
     8281 cgagcgcgga cgatctcgcc gacccgttcc atctggcatt cgtcgccgcg gcctgtgtgt
     8341 gtggaaaaca cttgcagcgg tccccgcagc gtggaaagct cagcttggag caagatgcgg
     8401 ggctccagcc atttcttgca gcgcggcaaa tcgtagacat gggatgccgc gatgggaaat
     8461 cgactcagaa tcgcagagcc ctccttgaac cccatcatgc cgacgacaat gctgtccagc
     8521 ggccacaagc cgaagacatg ttcggtggcc ggttcaaaga ccacgtggag gccgagcgcc
     8581 ttggcgagac gctccggaac attcccgtgt ctgcggctgt ccgacgcctc ttgcactgcg
     8641 atgatatcgg gatcaagtgt cttcagttcg cggatggtca tctccagtcg ttcctcgaga
     8701 tgcgtatcgc cgtcgaggaa gcctgagccc gctccatcgt gcaacaggtt gaacgtcacg
     8761 acgcgcaacg gacgaaggcc gttctgcgat tcggcaatag cctggactga acaatcaccg
     8821 gtcagatacg cactcaggag aagaagcgtc ggtaccgacc actgcgtcag gcgtggtgaa
     8881 agaccagggt ggagggtcac gtcatgcgcc atcccgtgat tctactcttt tctgcttgcc
     8941 tggttggcgg gtggctttcg attgttccct gcatggctgc tcatggcgat gcggatgccc
     9001 atggaatgaa agtaggccaa tggaccggag gagcgggagt aggctttctc ggcaatactc
     9061 ctgacggcgt cttggagttc gggctcgacg ggcatgtcga gtatgtcatt gccgagcgat
     9121 tctcgatcgg tccaatggtt cagtatgcgg ggaccggcaa cgactttctg ttcggtctct
     9181 cagcccaagt caagtactgg tgggacattc cgggaacgca aggcgccacc aagctggtgc
     9241 tccaaggcgg gctgggattt gtccgtgccg gcatcaccga cacggacagc gggacgggga
     9301 atacgtatgg atcgttcttg attccgatcg gcgttggcgt cgatcatgcc atgaggcctg
     9361 gaatggttct gacagccgat ttgctcctca acttcacatc cgtcggcgaa accgttcgcg
     9421 ccggcggccg tgaattcgat ctccacacca atattatgcc ggcgttctat ctgggggtcc
     9481 gcttctgatg aggccagttg ttcgaggcgc ggcaagatct ttcatggttg cgggcgaacg
     9541 aactttcgat agacgaccgg catcagaaag agcaatccga gtaccgtaaa cgccaggagc
     9601 agtcggggag aggccagctc cgccagcgag ttgatggtgc cgagctggcg cccggcaaag
     9661 gtgtaagcga ggcttcccgg aatgatacca agcgcagtag ctgccacata ggtgccgacg
     9721 ttgattctcg tcagcccgga gagcagattc accacgaaaa agggaaatat cggaaccaag
     9781 cgcagcgtga gcaggtacag aaaggcgttc cgtgcaaatc ccgcctggaa tgccgcgagc
     9841 cggtctccaa cacgttgttc gacccaatca cggaacaggt agcgggcggc gagaaaggcc
     9901 agcgtcgctc ctgttgtagc tccgatgttg acatacaggg cggccaacag ggttccgaag
     9961 aggaatccac ccatcagtgt gagtagggtt gcgccgggaa gcgagaaggc tgtttgtgca
    10021 atgtatacca gtatgaacaa taagacggcc tgtgcgtaat gctcctcggt aaaggacaaa
    10081 aacttgtcgc ggttcgcttt caacatgtcc agcgagaggt acgccatgag atcaaaaaaa
    10141 tagaacgcgc agatgactgc aaggaagacg gctgcgacga tgaacttgcc acgagcggag
    10201 ggccggctcg aggattcggg tatcggcacg tgccggtcac gagacattgc aaccctcctc
    10261 aattgtccgt acagtagttt acccagatgg atgcttcggg catcgtacct caccgtgtga
    10321 ggtgcagacc gccggccccg ccaatgattg ccggtttgcc gcgggatgtc gccgtcactt
    10381 ttctgaacat tgaggtgtgc tggtgccttg atggataggg gtggatgagc cgccatcata
    10441 atggaccact tcctgacact caactgcccc gaggtaccat gaagctgtcg tcgccggcga
    10501 gtgattccaa gaaagcgagg gtgcctatgg tggttgtatc attgttggtg gcattgctgg
    10561 tcgttccggt catcgttcac gcccatgacg agacggcgga caaaccgtct attacggttt
    10621 cgggccacgg ccacattgcg ctggcccccg atacggcctt cgtcacgctt ggcatggaga
    10681 cggtaggcaa gtccgttgga gaggcccagc ggcagaaccg ccaggcgatg aataaggtcc
    10741 tggaacggct tcgtggcctg cagatcgagc aggaacggat tcagacggcc tcgttcacag
    10801 tgtcgccgca gtacaagcca ccgccaaaac gtacggccga tgcaccgccg gcgcctcctg
    10861 agatcatcgg atacatcgtc agcaccaacc tgacagtcga agtgcggaat ctcgaaaagg
    10921 tgggagcggt gatcgaagag tccttggccg ccggtgccaa tcgttttcaa gcgttgcact
    10981 gggggcttct cgatgagcag caggcgaagc taagtgctct caagcaggcg gcggttaagg
    11041 cgcgtgagaa agccaccgct ttaagcgagt ccttgaaggt gaagctgatc cgactgatga
    11101 gcgccaccga ggacagtcat ctcgtgcgtc ctctgcctaa gctcgcacgg tcgatgatga
    11161 tggaaggggg gagcgaggaa acgcctgtct ccgcgggcga aataaagatt gatgcaatcg
    11221 tcaccttgaa ctatgaggtt ggccaggact aagcactagg gatttttgga ggtgacactg
    11281 gtcattttac cagcgcccgt cggtacggac ctttgataag ctgaaattac tttagaagac
    11341 ggcaatggaa acattgccaa aatccgaggt aggagcccgc tcccgaaagg aagtgggctc
    11401 ttttttttgg ccatttccaa ttcgcttcca cagcatccga gcctctcttg ggctgctacc
    11461 ggtcgccttt ttctcatcta cctttcagat ggcgcgctga ctgatctcca actgcgcgca
    11521 tgcaccaagc accgccctcc gcttccagca gggagcatgg caggatgttt tgcgacctgc
    11581 gacgggttgc atgacctttc tcatctctat cgttccgatg acggtctgat tgatctccaa
    11641 ctgcgcatgc accgcgcctg gcccaatgaa cagcttactt gctatccctt agatgaatga
    11701 tgcgcatgtc gctcccgctg cgtcttagat ggtccttcca agcttgctac tgatcctctc
    11761 ctcgacaagg ctgattagcc tctttgcacg atcattcttc acccgcccag accctggcga
    11821 ttcttttcga ccctcttgac ccacagatt
//
LOCUS       DASSCQ010000151         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1539255, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000151 DASSCQ010000000
VERSION     DASSCQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539255"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..629
                     /locus_tag="VFH05_10560"
     CDS             231..629
                     /locus_tag="VFH05_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6678704.1"
                     /translation="MAEKLQVKTGSLKEVVDLTDRLQALIGRAKMRDGLCTVFVTHTT
                     AALTTGEIGEGTEQDLLEVVQQVIPSIRFRHAHDPSHAWSHMAASILGPSLVIPVSDG
                     QLTLGTWQSVLLVELDGPRERNVYVTLLAS"
     gene            801..2267
                     /gene="zwf"
                     /locus_tag="VFH05_10565"
     CDS             801..2267
                     /gene="zwf"
                     /locus_tag="VFH05_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6678705.1"
                     /translation="MAEPSLEPHLFIILGATGDLTRRKLLPALFHLRTYGELEKQNTV
                     IVGAAQPEMSEEAFRLWAYEGLNNSVARHAPDLRKWCNEYLHYQTVRRGGPEDYQHLA
                     QYVRQLEVARNLPENRVFYLALPPTAVPPTVELLDQAGLLKSRGWVRVVFEKPFGHDF
                     HSARALNTLLHRYLDESQIYRIDHYLGKETVQNLLAFRFANPIFESLWNRDSVENVQV
                     TVAEDIGVEHRGAYYQEAGALRDMVQNHLTQLVTVVAMEVPVSFEAGAIQAEKLKVLH
                     SISPIPLSEVVFGQYQAWQIADQRIPGYREEKGVPPDSMTETFVALRMEIHNWRWRGV
                     PFYLRTGKRLPRKITQVGVTFRPAPIQVFRSLEPGSLDPNKLVITLQPSEGFALGFSV
                     KTPGRPFTFTSRALQFDYKQAFGGELPEAYETLLRDIMIGDQTLFVTADFTETAWRLY
                     DPLLAGKREVHPYTAGTWGPVEADQLLERYGHRWQLGW"
     gene            2271..3734
                     /locus_tag="VFH05_10570"
     CDS             2271..3734
                     /locus_tag="VFH05_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6678706.1"
                     /translation="MVEPPPFIVTDIPQRMDRLPWSKWHWLVVSALGITWLLDGLEVS
                     IVAALGPILTHDSTLGLTESQVGLAASAYLTGSVVGALVFSYLTDRQGRKKWFMITLL
                     LYLAATILTALAWDLPSLMFFRFLTGAGIGGEYAAINSAIDELIPARRRGHTDLAING
                     SWWLGSATGALLSLVLLNPRIFPESVGWRLCFVFGAVLGIAIVLVRRFVPESPRWLMT
                     HSRIPEAEEVVARIERQVTQDERFKVLAPPRGSITIRTRPHGTTISTVARELFRSYPR
                     RTALGLTLMVTQSFMYNAVSFTFPFVLTKYYDAPTSTIGLYLLPFALGNFLGPLLLGR
                     FFDTIGRKPMITSTYAIAGVLLAVTGYLFWTEAFTITTHLLAWSSIFFFASAGASAAY
                     LTVSEVFPMEIRAMAIAFFFMVAQGAGIVAPWLYGRMIETSVTSVYYGYLLGAGLMLL
                     GALAELVLGVKAEGRSLEQLAAPLSAQHISEDTSSAA"
     gene            3891..4145
                     /locus_tag="VFH05_10575"
     CDS             3891..4145
                     /locus_tag="VFH05_10575"
                     /inference="COORDINATES: protein motif:HMM:NF021169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HET6678707.1"
                     /translation="MQTVHVFDTWVTTPDSTLHFDVMTADQSTALRLANAYLAAQGHT
                     TISVTAEECRFCHAEPLVLFSEQQQREFRDCGGFIVPLSA"
     gene            4198..4641
                     /locus_tag="VFH05_10580"
     CDS             4198..4641
                     /locus_tag="VFH05_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HET6678708.1"
                     /translation="MGKGIVSAFVLCLIVSTGTTAGAEGGSKGKSLYERLGGKGAITA
                     VVDTFVGKVGADTRINGYFASTDLVKLKTHLVNQICEASGGPCQYTGRTMKQTHAGMG
                     VTDAAFGALVEDLVAALDHHKVGKAEKDELLGVLGPMKKDIVEKP"
     gene            4806..>5813
                     /locus_tag="VFH05_10585"
     CDS             4806..>5813
                     /locus_tag="VFH05_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET6678709.1"
                     /translation="MARRLSPAKIMQLGIGFWGSKALLSAIELGLFTELAKGPRDADT
                     LRQQLGLHPRSSRDFFDALVALGMLKRSGQRYANTPETAWFLDRNKASYVGGMLEMAN
                     ARLYRFWGSLTEGLQTGKAQNEVKTGEDFFGTLYSDPQRLEGFLKAMTGLSIGSARII
                     AKKFPWKQYRTFVDVGCAQGGVAVEIALAHKHLTGFGMDLPVVQPVFEAYAKARGVAS
                     RLRFTAGDFFKDPLPHADVIVMGHILHDWDLRDKMMLLHKAYEALPDKGALIVHEALI
                     DDERRTNAFGLLMSLNMLIETHGGFDFTGADCRKWMKEAGFSRTQVASLAGPDGMVIG
                     FK"
BASE COUNT         1139 a         1653 c         1738 g         1283 t
ORIGIN      
        1 ccctgtgaag ggcctcgctc aagggcgcag tcggaccagc atcgggcgcc cttcccgacg
       61 cggactgagg gaacaggtgc tcggtcaagc gcgcagtgag ggatagatcc agaccgcgcc
      121 tggccaacac ctgcgaatcc gctacaccac agtgtattcg ttcatttgcg tcgggtcttc
      181 ttcaggcacc cccttgtcta cgatcgtgag ctggtcaatg ggtgaatgga atggcggaaa
      241 agctccaggt caagacggga agtctgaaag aagtcgtcga tctcaccgat cggctccaag
      301 cgctgatcgg tcgtgcaaaa atgcgggacg gcttgtgtac cgtctttgtc acccacacca
      361 ccgccgcctt gacgacaggg gaaattggcg aggggacgga gcaggatttg ctcgaggtag
      421 tgcaacaggt gataccttcc atccgcttcc gtcatgcgca tgatccgtcg catgcctggt
      481 ctcacatggc cgcgtcgatt cttggtcctt cgctcgtgat tcctgtgtcg gacggacaat
      541 tgactttggg cacctggcaa tccgtgctgt tggtcgagtt ggacggccca cgtgaacgga
      601 acgtctatgt gacgttactc gcctcgtgat tgctcggcgt catcctgttg gttaggtctc
      661 cgccacataa gcgctccact tcctccattg gcccggttcc cttggtcaga gccatcctgg
      721 acgctcaggg gtttggtggg ttgacctggt caaccactaa atcgtacggt cagagagacc
      781 atacatgaga aagggagacg atggcggaac cttcgcttga accgcatctg tttatcattc
      841 tcggcgcgac cggcgatctg acgaggcgca aactgttgcc ggccttattt catcttcgca
      901 cctacgggga attagaaaaa caaaacaccg tgattgtcgg ggccgcacag cccgaaatga
      961 gcgaggaggc gtttcgcctc tgggcctacg aaggcctcaa caattccgtc gctcggcacg
     1021 cgccggatct gcgtaaatgg tgcaacgaat atcttcacta tcagaccgtc cgccgcggtg
     1081 ggcccgaaga ttaccagcac ctcgcccaat acgttcgaca attggaagtg gcccgcaatc
     1141 tcccggaaaa tcgggttttc tacctggcgc tgcctccgac ggcggtgccg ccaacggtcg
     1201 aacttctcga tcaagcgggg ctcttaaaaa gtcgggggtg ggtgcgggtg gtattcgaga
     1261 agccgttcgg tcacgacttt cattctgccc gcgcattgaa tacgctcctg catcgttatc
     1321 tcgatgaatc tcagatttat cggatcgacc actatttggg caaagagacc gtccagaatc
     1381 tgttggcgtt tcgtttcgcc aatcccattt tcgaatcgct gtggaatcgg gattctgtgg
     1441 agaacgtgca ggtgacggtc gccgaggata tcggagtcga acatcgcggc gcgtactatc
     1501 aagaggcggg tgccttgcgc gacatggtgc aaaatcatct cacgcagttg gtcaccgtgg
     1561 tcgccatgga agtgccggtg tcgtttgagg ccggggcgat ccaagcggag aagctcaagg
     1621 tcttgcattc gatttctccc attccattaa gcgaggtcgt cttcggtcaa taccaggcct
     1681 ggcagatcgc cgatcagagg attcccggct atcgagaaga gaaaggagtg ccgccggact
     1741 cgatgacgga aacgtttgtg gccttgcgaa tggaaatcca caactggcgc tggcgcggcg
     1801 tcccctttta ccttcgaacc ggaaagcggc tgccccgcaa gatcacgcaa gtcggcgtca
     1861 cgtttcgacc ggcgcccatt caggtcttcc gctctctgga gccgggcagt ctggatccca
     1921 ataagctggt gattacgctt caacccagtg aaggattcgc actcgggttt tccgtcaaga
     1981 ctcctgggcg tcccttcacg ttcacaagcc gcgcgctgca gttcgattac aagcaggcgt
     2041 tcgggggaga attacctgag gcctacgaga cgttgctgcg ggacatcatg atcggcgatc
     2101 aaacgttatt cgtgacggcc gatttcacgg aaacggcttg gcgactatac gacccgctgc
     2161 tggccggcaa gcgcgaggtg catccctaca cggctgggac gtgggggccg gtcgaggcgg
     2221 accaactgct ggaacgatac ggccatcgct ggcaattggg atggtgacgg atggtcgaac
     2281 cgcctccctt cattgtgacc gatattccac agcggatgga tcgcctgcct tggtcgaaat
     2341 ggcactggct tgtcgtgtcg gctctcggga ttacctggct gcttgatgga ctcgaggttt
     2401 cgatcgtggc ggcgctcggt ccgatattga cgcatgactc cacgttggga ttgacggaat
     2461 cacaggtggg attggccgcc tccgcctatc tgaccggttc ggttgtcggg gcgctggtct
     2521 tttcttattt aacggatcgg caaggccgaa aaaagtggtt catgattacg ctgctcctgt
     2581 acctcgcggc gaccattttg accgccctcg cgtgggacct gccaagcttg atgttctttc
     2641 gatttctgac cggggcaggc atcggcggcg agtatgccgc gatcaattcc gcgattgacg
     2701 agttaatccc ggctcgccgt cgagggcaca ctgatctggc gattaacggg agctggtggc
     2761 tcgggtccgc gacgggggca ttgctctcac tcgtgctgct gaatccccgt atttttcccg
     2821 agtcagtggg gtggcggctg tgcttcgtct tcggagcagt cctcggtatt gccatcgtgc
     2881 ttgtccgacg tttcgttcct gagagtcctc gctggctgat gacacatagc cggattccag
     2941 aagccgagga ggtggttgcc cgcattgaac ggcaagtgac gcaagatgag cgcttcaaag
     3001 tgctggcccc gccgagagga tcgattacga tccggacccg gccgcacggg acgaccatca
     3061 gcaccgttgc acgcgaactg tttcggtcct acccgcgtcg aacggcgctt ggattgaccc
     3121 tcatggtgac gcaatcgttc atgtacaatg cggtgtcctt cacattcccc tttgtcctga
     3181 caaagtacta cgacgcgccg accagcacca tcggcctcta tctcctgccg ttcgcactcg
     3241 gtaatttcct gggacccctg cttctcgggc gcttcttcga tacgattggc cgtaagccca
     3301 tgatcacctc cacctatgcc atagccgggg tgcttctcgc cgtcaccggc tacttatttt
     3361 ggacggaggc ctttacgatc acgacccatc tgctggcctg gtcgtcgatt ttctttttcg
     3421 cctcggccgg cgccagcgcc gcgtatctga cagtgagtga agtgtttccc atggagatca
     3481 gagccatggc aatcgccttc ttctttatgg tggcccaagg ggcggggatt gtcgcgccct
     3541 ggctctatgg caggatgatc gagacctcag tgaccagcgt ctattatggc tatctgctcg
     3601 gagcaggctt gatgctgctg ggcgcgctcg ctgagctggt gttaggcgtc aaagctgagg
     3661 gacgctcgct ggaacagctt gccgctcctt tatccgccca gcacatcagc gaagacacgt
     3721 cctccgccgc ctgaggcacg cctccaccgc cagaatcgac ccgtacaccc tcgcgaccat
     3781 ttcagtccct caagttgaaa acgatggcag tcatcccccc gctgtaggag gggcttgacg
     3841 ggggcttcgg cgcctgctat tagaaaggaa tcctaattaa tggaggtcgc atgcagactg
     3901 ttcatgtatt cgatacgtgg gtcacaaccc ctgattccac tctgcacttt gacgtcatga
     3961 ccgccgatca atccacggct ctccgactcg ccaatgctta ccttgcggcc caaggccaca
     4021 cgacgatctc ggtgacagca gaggagtgcc ggttttgcca tgccgagccg ttggtcctgt
     4081 tcagtgaaca acagcagcgt gagtttcgtg actgcggggg atttattgtg ccgttatccg
     4141 cgtgaaggcg gaacggtgtc gggctcgatc agtaccacca ccacaaggag ggcagctatg
     4201 ggcaagggca tcgtcagtgc gttcgtgctg tgtctcatcg tctcaacggg aaccaccgcc
     4261 ggggctgagg gtggctccaa ggggaagtcc ctctacgaac gactcggagg caagggtgcg
     4321 attacggcgg tcgtcgatac cttcgttggc aaggtcggtg ccgatacgcg tatcaacggg
     4381 tattttgcga gcactgatct ggtgaagtta aaaacccacc tggttaacca aatatgcgaa
     4441 gccagcgggg ggccatgcca gtacacggga cgcacgatga agcagaccca cgccgggatg
     4501 ggggtcactg acgccgcgtt cggagccctc gtggaagatc tcgttgccgc tctcgatcac
     4561 cacaaggtcg gcaaggcgga gaaggatgaa ttgctcggcg tgctcgggcc tatgaaaaag
     4621 gacatcgtcg agaagcccta gaccatcatg cggccgggag gtctcgacct cccggttatc
     4681 gtgtcgtcta ggtcaaccag gtctctcgga ttcctcttca gatctatcgt ggtcatccct
     4741 gcctgttgac agccagccgg gcggtgcgta gcctgtctct attttctgca gaaaaaggga
     4801 cgactatggc cagacgcctt tctcccgcaa agatcatgca actcggcatc gggttttggg
     4861 gatccaaggc gttgctgagc gccatcgaac tgggactctt caccgaattg gccaagggac
     4921 cgcgggatgc ggatacgttg agacaacagc taggactgca cccgagaagt tcgcgcgact
     4981 tcttcgatgc gctcgtcgct ttgggcatgc tcaagcggtc gggacagcgg tatgcgaaca
     5041 caccggagac tgcctggttc ctcgaccgca acaaggcttc ttatgtcggc ggcatgctgg
     5101 aaatggccaa cgcgcggctc tatcgttttt ggggctcgtt gacggagggc ttacaaaccg
     5161 gtaaggcgca aaacgaagtg aagacgggcg aggacttttt cggcaccttg tattccgatc
     5221 cgcagcggct tgagggattc ctcaaggcca tgacggggct cagcatcggg tcagcgcgga
     5281 ttatcgccaa aaagtttccc tggaagcagt accggacgtt tgtcgatgtc gggtgtgcgc
     5341 aaggcggtgt ggctgtcgag attgcactgg cgcacaagca cctcaccggc ttcgggatgg
     5401 atctgcccgt cgtgcaaccg gtgttcgagg cgtatgccaa ggcgagaggc gtcgcctcac
     5461 gcctgcgttt caccgccgga gatttcttca aagacccgct cccgcatgcc gacgtcatcg
     5521 tcatgggcca catcctgcac gattgggatc tccgcgacaa aatgatgctc cttcataaag
     5581 cgtatgaggc ccttcccgac aagggcgccc tgatcgtgca cgaagccttg atcgacgacg
     5641 agcggagaac gaatgccttt ggcctgctca tgagtctaaa catgctcatc gagacgcatg
     5701 gcggtttcga ttttacgggg gccgactgtc ggaagtggat gaaggaagcc ggtttcagcc
     5761 ggactcaggt cgcgtcattg gcgggaccgg acggcatggt gatcggattc aag
//
LOCUS       DASSCQ010000152         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1215437, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000152 DASSCQ010000000
VERSION     DASSCQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215437"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="VFH05_10590"
     CDS             complement(<1..1609)
                     /locus_tag="VFH05_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET6678710.1"
                     /translation="MLRPTLADVLVAQGMVTQSTVDHTLNKLGGVSADLGATLLGEGA
                     LSEDQLARALASQYGLPFDPLVDFRVEQSFYERMSVKLMKRHPFVPITECEGRLKVAV
                     PDPQDLLGIDELELLLGRELELVVSPKSAILAALEHSEGSTQALRELEAEYRSVLVKE
                     DERGEEILTLDRSGEDQSPAVKLLDSIFLSAMQRRASDIHIEAADRSTRVKFRVDGIL
                     VPAMEPLDIRLHAPLVSRLKVMSDLDIAERRVPQDGSFRMRLERKTVDFRVSILPSVF
                     GESAVIRILDREAITAGVSTLRLDRLGFNDEDLKRFRRAITRPYGMVLVTGPTGSGKT
                     TTLYAAITEMNIKEDKLITIEDPVEYQLPGVVQIPVNEKKGLSFARGLRSILRHDPDK
                     IMVGEIRDSETAQIAIQSALTGHLVLTTVHANNVFDVIGRFASMGIDSYNFLAALKCV
                     LAQRLIRMVCQSCRAPVALEKALAEESGLDYEEYKDVPFYQGTGCHECHGTGYKGRKC
                     ITEFLDLTDEIKEMILSERPLSEIRYRA"
     gene            complement(1677..2888)
                     /locus_tag="VFH05_10595"
     CDS             complement(1677..2888)
                     /locus_tag="VFH05_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET6678711.1"
                     /translation="MAVFAYRVARPDGSTLEGQMEGDEEPLVRAKLEGQGFLIFQLKR
                     RGVASSRPAIDLSGFRGLPISEFLVFNQELLALVKAGLPVLRVWDLLIERARHRGFQQ
                     ALRMVRQDIRGGASASEALTRHPGYFSELYVATIRAGEQSGNLVEVVKRFIGYLKLMI
                     GLRQKVGKALAYPAFLVLTGVAVVGFLLSYVMPTFISVYTETAKSLPFATQMLIDVVS
                     SARLYVIPVTALLVGLLLLGRAYYSTAHGRMRVDQLLLRAPVLGAVFVKHHTIQLART
                     LGTILAGGTPLVEALQITKSAVSNRYIAVGLTEAVNEIREGTTLASALERPKVFPKLA
                     IEMLSVGEETGSLEAMLQDVAEFYEADLDLRLTQLTTWIEPALLLVMGILVGGIVIVM
                     YLPVFQMAGNV"
     gene            complement(2939..3694)
                     /locus_tag="VFH05_10600"
     CDS             complement(2939..3694)
                     /locus_tag="VFH05_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET6678712.1"
                     /translation="MSPDNTAHHGLRGNDSRTDCSAVRGIALCILAVVALVASACGEL
                     EPMVEPEMVDLQLTLDTLRSQVREAQRNLAEVRTELESRRQELAEAQVARAQLEGRVR
                     EAERRVTEARQVIELQREELIVAKTERERVFRSSSQQLKPLQRRGVKTGGLDSADWGG
                     SAVPTDASLPWFFPNPSRSATQAMNGQSSKGTASAVAVRRQPSLTEIVVKPGDTLYSL
                     ARTYRMTLSRLRSLNHVSDDQIIVGQILLVTEK"
     gene            complement(3703..4104)
                     /locus_tag="VFH05_10605"
     CDS             complement(3703..4104)
                     /locus_tag="VFH05_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6678713.1"
                     /translation="MERNTEIGEFRLAGAPGFTLIELLIVVSIIGILATLAAPSYQTS
                     VVKAKEAALRQDLFTLRDVLDQHRADQGKYPASLGALVSAGYLRAIPKDPFTGSTTSW
                     QEMTDEMEGGTFDVFSGSELVGTNGTPYNRW"
     gene            complement(4079..4570)
                     /locus_tag="VFH05_10610"
     CDS             complement(4079..4570)
                     /locus_tag="VFH05_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678714.1"
                     /translation="MTLLELLVTLTIVMILASVAMPLSRVSTKRSHEIELRQHLRIMR
                     AAIDTFKAEWNRDGDVLIGPLCVKNKLSCKDVTSIYGYPKSLEMLLGVKLTSEEATLR
                     GTTIRRYLRSLPLDPLTGEANWQFRCYKDPPNASSWCGSDVYDVMTVSQETALDGTKY
                     RDW"
     gene            complement(4582..>5298)
                     /locus_tag="VFH05_10615"
     CDS             complement(4582..>5298)
                     /locus_tag="VFH05_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678715.1"
                     /translation="IPWIGDLPFIGNLISSFKTQRVTTEVILTLTPHIVQAMTPPSLS
                     RQAFWSGTDSIYSNSTMFTTQQPKATPTRITRAQVSSPASLPMAQRGLAAKVPSGALT
                     KMLTVGPIASIKPNETIVEVGKEFKIAIQDDRLRPERDGLFHLQYDPNVLDLKKLIDA
                     EQIQLDPAELPPSAVAEAVVAFRVGASAPRASTGGRTVTARFRAKAPGVSPIRVALVN
                     ANGEPTADASPEGRGIVRVR"
BASE COUNT         1138 a         1625 c         1411 g         1124 t
ORIGIN      
        1 ccgctcgata gcgaatctcg gagagcggtc gctcggacaa aatcatctct ttgatctcat
       61 cagtgagatc cagaaattcc gtgatgcatt ttcggccctt atagccggta ccatggcatt
      121 catgacagcc ggtcccttga tagaacggca catccttata ctcttcgtaa tccaggccag
      181 attcttccgc cagcgccttc tcgagcgcga ccggtgcacg gcaggactga cacaccatcc
      241 ggatcaagcg ctgggcgagg acgcacttca atgcggcaag aaaattgtac gagtcaatcc
      301 ccattgaagc aaaacgcccg atcacatcaa agacattgtt ggcgtgtacg gtcgtcaaca
      361 ccagatgacc ggtcaaggcc gactggatgg cgatctgggc cgtttccgaa tcgcggattt
      421 caccgaccat gatcttgtcg ggatcgtgac gcaaaatcga gcgcaggccc cgcgcgaagg
      481 acaggccttt cttttcattc acgggaatct ggacgactcc gggaagctga tactcgaccg
      541 ggtcttcgat cgtaatgagc ttgtcttctt tgatgttcat ttcagtgatc gcggcataca
      601 gagtcgtcgt ttttccgctt cctgtcggac cagtgacgag caccattccg tacggtctcg
      661 taatcgccct gcgaaaccgt ttcagatctt cgtcattgaa gcccagtcgg tccaggcgca
      721 aggtggatac gccggccgta atggcttcac gatccagaat acggatgacc gccgattctc
      781 cgaacacact gggaagaatg gagacgcgaa aatcgaccgt cttgcgttcc aaccgcatgc
      841 gaaaactgcc gtcctgcggg acacgacgct cggcaatatc gaggtcagac atgactttca
      901 agcgagagac gagtggtgca tgcagccgaa tatccagcgg ctccatcgcc gggaccagaa
      961 tgccatccac acggaacttc acgcgcgtcg aacgatcagc cgcttcaatg tgaatatcgc
     1021 tggcacgccg ctgcatcgcg ctcaagaata ttgaatccag cagtttgacg gcgggacttt
     1081 gatcctctcc gctgcggtcg agcgtgagaa tctcttctcc ccgttcatcc tccttcacca
     1141 gcacggaccg atactccgcc tcgagttccc gcaaagcctg agttgatcct tcgctgtgtt
     1201 ccaatgcagc aaggatcgcc gacttcgggc tcacaaccag ctccagctcc cggcccagca
     1261 ataattccag ttcatcaatg cccagcaggt cctgtgggtc aggaactgca actttgaggc
     1321 ggccttcaca ctctgtgatc ggcacaaatg gatgccgctt catgagcttg accgacatgc
     1381 gctcgtagaa cgattgctcc acccgaaaat caaccagcgg atcgaagggc agtccatatt
     1441 ggctggcgag tgcacgcgcc aactgatcct ccgacagcgc cccttcgccc aacaatgtgg
     1501 ctcccaaatc cgccgagacg ccgccgagct tgttcagggt gtggtccacc gtcgattgag
     1561 tcaccatccc ctgtgccacc agcacatcgg ccagtgtcgg tctcagcatg cttcgagctg
     1621 ccaccgcacc tctcttggga ctcgaatgtc gctcgctcag atagctcatg aatcgctcac
     1681 acgtttcctg ccatttggaa gaccggcaaa tacatgacga tgacgattcc tcctacgaga
     1741 attcccatca ccaataggag cgccggctcg atccacgtgg tcagttgtgt caaacggaga
     1801 tcgagatcgg cttcataaaa ttcggccaca tcctgtaaca tcgcttcgag agagccggtt
     1861 tcctcgccga cggacaacat ttcgatcgcg agcttcggaa atacctttgg tcgctccagt
     1921 gccgaggcga gtgtcgtacc ctcacgaatc tcattcaccg cttcggtcaa tcccacggca
     1981 atgtatcggt tcgagactgc gctttttgtg atctgcaatg cttcaacgag aggcgtccct
     2041 ccggccagta tcgtccccaa tgttcgcgct aactgaatgg tgtgatgctt gacaaagacc
     2101 gcccccaaga ccggtgcacg cagcagcaat tgatccaccc gcatgcggcc atgagctgtt
     2161 gagtagtagg ctcggccgag caacaggaga ccgacgagca gcgcggtgac ggggatgacg
     2221 tacagtctgg cgctcgacac gacatcgatc aacatttggg tggcgaaggg cagggatttg
     2281 gcggtctctg tgtagacgga aatgaacgtc ggcatgacat aactcaacag aaatccaacg
     2341 acggcaacgc cggtcaacac caaaaaggcc ggataggcga gcgccttgcc gactttctgt
     2401 cgaaggccga tcatcaactt cagatagccg atgaatcgtt tcaccacttc caccagattt
     2461 cccgactgtt cccccgcccg tatcgtggcg acatacagct ccgaaaaata gcccgggtgc
     2521 ctggtgaggg cctcggaagc cgaggctccg ccgcggatgt cctgacgcac cattctcaat
     2581 gcctgctgaa aaccgcgatg gcgtgcgcgc tcaatcagca gatcccacac gcggaggacc
     2641 ggaaggcccg ccttcaccag cgccaacagt tcctggttga agaccagaaa ctcgctgatt
     2701 ggaaggcctc gaaatccgct gaggtcaatc gccggccgac tggaggcgac gcctcgacgc
     2761 ttgagttgaa agatgagaaa tccctgcccc tcgagcttcg cccggacgag cggctcctcg
     2821 tcaccctcca tctggccttc gagcgtcgat ccatcaggac gcgcaacccg gtacgcgaat
     2881 acagccataa gaatttctca tgaatgaggg gcttgccgat cgccgccctg tggcacggtc
     2941 acttctccgt caccaacagg atctgaccga cgatgatctg gtcgtcgctc acgtgattga
     3001 gcgaccgcag gcggctgagt gtcatccgat atgtgcgcgc gaggctatac agcgtgtcac
     3061 ccggcttcac cacgatctct gtgagggaag gttgccgcct cacggccact gcactggccg
     3121 tccctttcga cgattgcccg ttcatcgcct gcgtcgccga ccgtgacgga ttggggaaga
     3181 accacggcaa cgatgcgtca gtcggaactg ccgagccacc ccagtctgca ctgtccagac
     3241 ccccggtttt tacacctctt cgttgcagtg gcttcagctg ctgcgagctg ctgcggaaga
     3301 cccgctcccg ctcggttttc gcgacaatca gttcttctcg ctgcagttcg atgacttgac
     3361 gggcttccgt aacccgacgt tccgcctctc gtacccgacc ttcaagttgg gcccgggcca
     3421 cctgggcctc agccaactct tgacggcgcg actccagttc agtccgcact tccgccagat
     3481 tccgttgggc ttctcgcact tgggatctca atgtatccag cgtgagctgg agatcgacca
     3541 tctccggttc aaccatcggc tccaactccc cgcaggcgct ggcgacgaga gccaccacag
     3601 cgagaataca gagggcgatc ccccgaactg cagagcagtc ggttcggctg tcattccctc
     3661 tcaggccatg gtgtgctgta ttgtcaggag acacgtgcat actcaccagc ggttatacgg
     3721 tgttccgttc gtaccgacta actctgagcc tgaaaacaca tcaaaggttc cgccttccat
     3781 ctcatcggtc atttcctgcc acgaggtggt ggagcccgtg aaggggtcct tggggatcgc
     3841 ccgcagataa ccagcgctca caagtgctcc cagtgaagca ggatatttcc cttggtccgc
     3901 ccgatgttga tccagtacat cacgcaaagt gaacaggtcc tgccgcaatg ccgcctcctt
     3961 ggccttgacc acggacgtct gataactcgg tgcggccaac gtcgccaaga taccgatgat
     4021 cgacacgacg atcagcagct cgatcaaggt gaaccctggc gccccggcga gacggaattc
     4081 accaatctcg gtatttcgtt ccatccaacg ccgtttcctg actgacagtc atgacgtcat
     4141 agacgtcgct cccgcaccag ctcgacgcat tgggcgggtc cttataacag cggaattgcc
     4201 aattcgcctc accggtcagt ggatcaaggg gaaggcttcg aaggtaccgc ctgatcgtcg
     4261 tgccccgtag agtggcttcc tcgctcgtca gtttgacgcc caaaagcatc tccaacgatt
     4321 ttggatagcc atagatgctc gtgacatctt tgcacgacag cttgtttttc acgcataacg
     4381 gaccaatgag gacatcgccg tcccgattcc attccgcctt aaacgtgtcg atagccgccc
     4441 gcatgatccg cagatgctgc cgcaactcga tctcgtgcga tcgtttagtc gagacccggc
     4501 tcaatggcat ggcaaccgag gccaggatca tgacaatcgt caacgtaacg agaagctcaa
     4561 ggagcgtcac tccttcctct ctcaccgcac cctcacaatg cctcttccct cgggtgacgc
     4621 atcagcggtc ggctctccgt tcgcattcac cagagcaaca cgaatgggag agacgccggg
     4681 cgccttcgcc ctgaacctgg ccgtgaccgt tctgccgccg gtcgaggcgc gtggagcaga
     4741 cgcgcccacg cgaaacgcga caacggcttc ggccacggcc gagggaggca gttctgccgg
     4801 gtccagttga atctgttctg catcgatcaa cttttttagg tcaagcacgt tgggatcata
     4861 ctgaaggtga aagaggccgt ccctctctgg ccgcaatcgg tcgtcttgaa tggcgatttt
     4921 gaattccttt ccaacttcta cgatggtctc attgggcttt attgaagcga tcggacccac
     4981 cgtcaacatt ttagtcaggg caccggacgg caccttggcg gcaagtcctc gttgggccat
     5041 aggtaaggat gcgggcgacg acacctgggc tctcgtgatt cttgtcggcg tagccttggg
     5101 ttgttgcgtc gtaaacatgg tcgaattcga gtagatcgag tcagtcccag accagaaagc
     5161 ctgtcggctc aaacttggag gggtcatggc ctggacgata tgtggggtca acgtcaagat
     5221 tacttcggtc gtgaccctct gggtcttgaa cgagctgatg agattgccga tgaaggggag
     5281 atcacctatc catggaat
//
LOCUS       DASSCQ010000153         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_202442, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000153 DASSCQ010000000
VERSION     DASSCQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202442"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..619)
                     /locus_tag="VFH05_10620"
     CDS             complement(65..619)
                     /locus_tag="VFH05_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6678716.1"
                     /translation="MLRQDMRWSHPAYWMLLCAFAVGCGSPKIGYDYDRSADFSRYHT
                     YGWIAGAQEATGDRRVHSSLVDARIRTAIDAQLRAKGYTVSANGRPEFLVAYQVGMKD
                     LIVGASTQNYIGDRVHGTYTTISDIQPYHEGTLLIDIVDAGSQILVWQASARAEVDQS
                     LGPKERDERISTIVRAMLAHFPPQ"
     gene            749..1684
                     /locus_tag="VFH05_10625"
     CDS             749..1684
                     /locus_tag="VFH05_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6678717.1"
                     /translation="MNCTKCKTKAVIKIPRHHAAFCKGCFTHFVHDQVARAVRSERMF
                     GKDDRILVAVSGGKDSLALWDILLKLGYKADGFYVNLGIGGYSERSYTMVRRFADSVA
                     APLGAALHTHTVEHEEGGGIKELAQVVHRPTCSTCGTIKRYQFNRAAVEREYDVMATG
                     HNLDDEAARLLGNVLHWQEDYLEKQGPTLPASIEGFAKKVKPLYRLSERELAAYCVLS
                     KIEYIVDECPMAQGARTLLYKEVLNRLETESPGTKQYFYWGFLDKQRRKQDAPLTMSE
                     KDQALLHPCATCGQPTTAEVCSYCKLMARAKLPAR"
     gene            1754..2791
                     /locus_tag="VFH05_10630"
     CDS             1754..2791
                     /locus_tag="VFH05_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET6678718.1"
                     /translation="MATTPRDFYQVLGLPRTASADDIKKAYRRLARQYHPDLHSGSKK
                     TEMEKKFKELNEANEVLSDPDKRKKYDQYGAQWEQAEAYEKARQQAGNQGFSWQQQQS
                     GGDFRGEDFSDIFENLFGGRGRAGTSNRGFSASGEDLEAEVELTLREVLSGVTKRVSL
                     REPVPCPTCQGSGALRGRTCPTCLGAGTTTELKTIEVKIPAGVQDGTRVRVAGKGQPG
                     SNGGKRGDLYLHVTIKPDPIFRREGSDIHVTLPVYPWEAVLGAEVMAPTLTEPVKVKV
                     PAGSKAEGKLRLKDKGLQSAGGGRGDLFLHLQIVVPPTVTEEEQTLYQRLARDQHPDP
                     RAELLKQTRMR"
     gene            2796..3431
                     /locus_tag="VFH05_10635"
     CDS             2796..3431
                     /locus_tag="VFH05_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET6678719.1"
                     /translation="MTPTEYGVLAFSSLFVIVDPLATVPAFLAMTARYTLSQRLRMAR
                     VACCVAFLILSGFALAGQWLFSVLGITLAAVQVAGALVLLLVALDMLRAQRSAVVETA
                     AETAEGAAKDDIAITPLAVPMLAGPAAISTIILLDAQAASWLYRGILLACVALVGLAS
                     YAILALGASGAKWMSPLAEKIITRLMGLLLAALAVQFLFNGLKGEGGLYMR"
BASE COUNT          746 a         1022 c         1031 g          700 t
ORIGIN      
        1 agaacaccaa tactcaagag tctccgtgtc atcaccgtca tgctcatgcg agtgcccgac
       61 acatttattg aggtgggaaa tgcgcaagca tcgcacggac gatagtgctg atgcgttcat
      121 cacgttcttt gggaccgaga ctctgatcaa cctccgcccg ggctgaggct tgccacacca
      181 gtatttgtga tccggcatcc acgatgtcga tcaagagcgt gccttcatgg tatggctgta
      241 tgtcgctaat ggttgtgtag gtaccgtgca ctcgatcgcc gatatagttc tgagtggatg
      301 cccctacgat cagatccttc attcccacct ggtaggcgac caagaactcc ggccggccgt
      361 ttgccgagac agtataccct tttgcacgca gttgggcgtc aatggccgtt cgaatgcggg
      421 catcaacgag agagctatgc acgcgccggt cgcccgtcgc ctcttgtgcg ccggcgatcc
      481 agccgtaggt atgataccgg ctgaaatctg cgctccggtc gtaatcgtaa ccaatcttcg
      541 gcgagccgca gcccacagca aaggcacaga gaagcatcca atatgcgggg tgactccatc
      601 tcatgtcttg acgcagcatg ggaacaacat tgccgtcctg tcaagagggt gatgacggca
      661 atcgagccct catgtccgct ccatcattgg tcaattcgta ctgacaccat atttgcattt
      721 gccataagtc cgctcgtaag atcgactggt gaactgcaca aagtgcaaga cgaaagcggt
      781 catcaaaatt ccccggcacc atgccgcgtt ttgcaagggc tgcttcacgc acttcgttca
      841 tgaccaggtg gcccgtgcgg tcaggtccga gaggatgttc gggaaagatg accgaattct
      901 ggtcgccgtt tctggcggaa aagacagctt ggcgctctgg gacattctgc tgaagctcgg
      961 ctacaaggcc gacgggttct acgtgaacct cggaatcggc ggctattcgg aacggtccta
     1021 cacaatggtg cgccgctttg cggactcggt cgccgcgcct ctcggcgctg cgttgcacac
     1081 acacacggta gaacacgagg aaggcggcgg gatcaaggaa ttggctcaag ttgtccatcg
     1141 tccgacttgt tcgacctgcg gcaccatcaa gcggtatcaa ttcaaccggg cggccgtcga
     1201 acgggagtat gacgtcatgg ccaccggtca caacctcgac gacgaagcgg cacgcttact
     1261 cggcaacgtc ttgcactggc aagaggacta cctcgagaag cagggaccga cactcccggc
     1321 ttcgattgaa gggtttgcga agaaggtgaa acccctgtac cggttgtccg agcgggagct
     1381 tgcggcctat tgcgtcctga gcaaaattga gtacatcgtg gacgaatgtc cgatggcgca
     1441 aggggcgcgc accctcctct acaaagaggt gctcaaccgg ttggagaccg aatctcccgg
     1501 cactaagcag tacttctact ggggattctt ggacaaacag cgccgcaagc aggatgctcc
     1561 gcttaccatg agcgaaaagg atcaagcgct gctccatcct tgtgccacgt gcggacagcc
     1621 gaccaccgcc gaggtgtgtt cgtactgtaa attaatggcg cgagccaaac taccggcgcg
     1681 ataagccaat gtgttgcata cgcagcgccc gtagcgcttg atctgtgcta acccggcatt
     1741 atctctctga cgcatggcca ccactccacg tgatttctac caggttctcg ggcttcctcg
     1801 aacggcctcg gccgacgaca taaagaaggc ctatcgccgg cttgcccgcc aataccatcc
     1861 cgacctccat tccggatcaa agaaaaccga aatggagaag aaattcaaag agctcaacga
     1921 agccaacgag gtcctgtccg acccagataa acgcaagaaa tacgaccagt acggggctca
     1981 atgggaacag gcagaagcct atgagaaggc gcgtcaacag gctggtaacc aggggttctc
     2041 ttggcaacag caacaatcgg gcggcgactt ccgcggtgag gatttttcgg atatttttga
     2101 gaacctgttc ggtggacggg gccgtgctgg aacatccaac cgcgggtttt ccgcctcggg
     2161 tgaagacctc gaagcggaag tcgagttgac cttgcgagaa gtgctgagcg gtgtcacgaa
     2221 gcgagtgagc ttgcgcgaac cggtcccctg cccgacctgt cagggaagcg gcgcactgcg
     2281 cggacggacc tgccccacct gcctcggtgc cggtaccacg acggaattga aaaccattga
     2341 ggtcaagatc ccggccggcg tccaggacgg cacgcgggtg cgcgtcgcag ggaagggcca
     2401 gcccggctcg aacggtggca agcgcgggga tctgtatctc catgtcacga tcaaaccgga
     2461 cccgatcttt cgccgcgagg ggagcgacat tcacgtcacg ctgccggtgt atccctggga
     2521 ggcggttctg ggcgcagagg tgatggcgcc gacactcacg gagccggtca aggtcaaggt
     2581 gccggcgggg agcaaggccg aaggcaaatt acggctcaaa gacaagggcc ttcagtcggc
     2641 cggaggaggt cgaggcgatc tcttcttaca ccttcagatt gtcgttccac cgacggtgac
     2701 cgaagaagag cagacgctgt accagcgatt ggcacgagac caacatccgg atccccgcgc
     2761 cgagctgctc aagcaaactc gaatgcgatg accgcatgac tccgactgaa tacggggtgc
     2821 tcgcgtttag ctctcttttc gtgatcgtcg atcctctcgc caccgtcccg gctttcctcg
     2881 ccatgaccgc tcgttacacc ctcagccagc gcctgcgcat ggcgcgcgtc gcctgttgtg
     2941 tcgctttcct gatcttgagc ggctttgccc tcgccggcca atggctcttc tcggtgctgg
     3001 gcatcacgct tgccgccgtg caggtagccg gagcgctcgt cttgctgctg gtggccttgg
     3061 acatgcttcg ggcgcaacgt tctgcggtgg tcgagactgc tgccgaaacg gccgaaggtg
     3121 ccgccaaaga cgacatcgcc atcacgccgc tcgccgtccc gatgttggcc gggccagccg
     3181 ccatctcgac cattatcctg ttggatgcac aggccgcgtc atggctctat cgaggcatcc
     3241 tgctggcttg cgttgcgttg gtcggtctgg caagctacgc catcctggct ctcggtgcat
     3301 ccggagccaa gtggatgagc ccactggcag aaaagatcat cacgcgtttg atgggcttgc
     3361 tgctcgccgc cttggccgtt cagttcctgt tcaacgggct gaagggcgaa ggcggtttgt
     3421 atatgcgata atgtcgtgac atgacaagaa accgtaggac gttcaaccga aggaggattg
     3481 catgaagaag gagacgcag
//
LOCUS       DASSCQ010000154        22163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1272979, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000154 DASSCQ010000000
VERSION     DASSCQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 22163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22163
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272979"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..300
                     /locus_tag="VFH05_10640"
     CDS             52..300
                     /locus_tag="VFH05_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678720.1"
                     /translation="MTRTTIITAAGAFMVPSFMSACNRDQDQTTPVGQQQGQGSAQDY
                     PSTRNMEQPNPAIGGTNSATNKPAPSGNQEPQAGSTTR"
     gene            449..715
                     /locus_tag="VFH05_10645"
     CDS             449..715
                     /locus_tag="VFH05_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET6678721.1"
                     /translation="MPNITLLISPSCGACPSAKSLWKQLRVKYSFSYREVDITTPDGQ
                     ELANRHAVRAVPATIIDGRLTFVGVPSRESAEKALQLKMRPREA"
     gene            1062..4220
                     /locus_tag="VFH05_10650"
     CDS             1062..4220
                     /locus_tag="VFH05_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6678722.1"
                     /translation="MLSVVTGRFQPALESALVTHIQGIKAVDVWSQIAIVVPSKPLLD
                     RIRWLLAVEHQLAFVNVHLLTFHQLALRLAEEIHREGTGHPPRVVDDLFFEYLVRHLV
                     QRRLAELPALRQLGRSFGTWGGLWSTVRDLNDGGVDPATVLRAVGEGYFDPDEADWLR
                     ALFSLHAAVREVGRGLHFVTADDLTEAVLPAVSTSPFLTSLSHIAYYGFYDLTQVQLS
                     MFQAVSGTVSTTLFFPLATDSSFDFARRFFDRHVQPLLKSNDALVEAAETGEAFARVD
                     VAVRSVVGREEELAVACRTILDLVETHGYRFDEIGVVARTLDPYHSAACNIFDRHRIP
                     FSTTARRPLSHEPLCKTLLALASLSANDYYRAAMLDLVTSPFYRVPHSSGRFERGPAY
                     RPEQWKLVLEALHITHGLDEWTRLKQASQSALEWEVEDEAAGTIGSVKIAPDVIGLLW
                     ETVSELLKSYSSLPARGTIGSLLPAFRELMANHVHRVEEEHAIWSAVDQACAAVNELD
                     AIGEDITWAEFAELLAHAFERVTIPLEATPHQGVRLLDAMTARGVPFKALFVLGLNEK
                     VFPRHVREDAFLRDRHRRVLDATLGFKIDEKLAGYDEERLLFSLICGSAVRRLYLSFP
                     RADDAGRMLAASPYIGGELRRLGFREVEVEMVPRRLSDRIKQRPTISRFLPPEDLAQW
                     MAGRGEDPAELLQATGRDAALYHRGVEALGLIEDDRAALTAFDGMTGPLKNHWARIAE
                     RGIAPTPLERYASCPFKYFSADVLRLDPVRVPPADEPDARLLGTVCHAALRRCYELLL
                     PTGWPVKPVTNDTIDWCVETAVEQAAGEQEILHRTGHYLLWQLAKSQIIDVVTAAVDD
                     DTRAYLDEPFIPILFEVDADGSIDDLPLNVPSPLRMRGRIDRVDRHRISGALRVIDYK
                     FKIGNSMKPQDRNLLLSAVRGYRLQPPMYARLNVPDHGRVHEVQLFFLAPHWPTPVVR
                     STFKTDLWSSDSGTSLRTTFALLVDGLRNGTFFILPDTYCETCDYRVACRREHTLTSW
                     RASRAAEAKALTLLRTSRVNDA"
     gene            4213..7557
                     /locus_tag="VFH05_10655"
     CDS             4213..7557
                     /locus_tag="VFH05_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET6678723.1"
                     /translation="MPNRSSLSDTAAREAAETTYDRNVVVVAGAGTGKTTLLVNRLVH
                     LLMREPDPVGLLQVVALTYTNKAATEMKIRLRERLTMLAQVPRPGETGPDGGTMAIED
                     LLLRYGITVEDIAGRATTALQDLEKAQIGTVHSFAGHLLRLYPLESGVDPAFREDDGT
                     RFNEHFRVAWDVWIDRELSRQGTQHKLWRALLESTTVAQLRTLTFCLCSDLIDIEALT
                     RQLEMESPSDLAQWIGRMATRSQALLDAYDRPKRRKIEHMLAAAATLMKLVRDEGPDA
                     TTQLDAAERAWLEKKTSDEVSGWEEHDFTEATKLIKLAQQLLLLDHHYFNKFLKLVSP
                     LITSVLATFADAGWLSFDGLLARARTLLWEHPAIRERVKREYRAVLIDEFQDTDPLQY
                     EIILAVSEEPRHHSVNWQSIVLEPGKLFIVGDPKQSIYAFRRADMEAFDRVVQKIEAD
                     GGIVHNLTTNFRSDASVLEPVNDIFDRLFVRRPLVQPGNVRLEGGERRRRSRLDPGVL
                     LQITASRPGDPPFDAAGATRAEAEALACWLREEVLENRQVSAGQIALLFRTLTQADAY
                     LDALRRYDIPYLIEGEKHFYRRQEVIDLVNVLRVLDHPHDHIALVGVLRSPLGGLTDR
                     DIYDLHEAGLFHYLNEAGTAQWSHPRADNVRRLYRRLTLLHQQVRTLPLPEAIQVIFD
                     ELPVLELAAASLHGEQAVANLLKVKQTATALLDRPYLTFSGFIDLMVTRLDEQPEEPE
                     SPLAEESSDAVQILTIHKAKGLEFSVVVLPGLHQGKGREGTSPSVVYDWSTGNYGLSL
                     GRLHTMGYLRVQQKLVERQEAERKRVCYVGMTRSKDLLLLSGAVASRSAGDTVLDWLH
                     DIGEGEIGNPMTGAWKVGSSAIPHRVVYAPERKSGRRPLSTHETVPPLHPATLAQLWD
                     ERTTRRGAVQGISWRLTPTSLNAIMPTHRAESGRTTADRDVGRLAGVLAHRILEGWDF
                     SRSPVELLDQIAPALDTELTPERLRPAVAASLKAIFTAFGWSDVYMRLRSATILGREV
                     PFIMPWGDRQVMEGVIDLIYRLDGKLWIADYKTDQTTAEEAPTKAAFYSYQADVYRRA
                     ATKCLGLPHVSFHLLFLRAGTSVELT"
     gene            7634..8047
                     /locus_tag="VFH05_10660"
     CDS             7634..8047
                     /locus_tag="VFH05_10660"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678724.1"
                     /translation="MHIVWSVLLLSAMFGCSGQSSKAISVLAAPAGTKSTLATQLEQG
                     NALFASSDYAGAEQAFRQALAIDATSAEAHYNLAVTLDRTGNKTEAKRHYIQAANLAP
                     GNKVIWDSPPLRAQTNGLNHDISRKSHLDPSNRGF"
     gene            8049..8432
                     /locus_tag="VFH05_10665"
     CDS             8049..8432
                     /locus_tag="VFH05_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET6678725.1"
                     /translation="MARPRRRDVSPEQFDLLVQQALAELPAPYARLAQEVSVVVEEEP
                     PPDVLEDLELDSADDLLGLYQGLPVAAESFFHTGGQQPPRIAIYRGPILRLCETAEDV
                     RREIRDTVVHELGHHVGLGDDDMPY"
     gene            complement(8457..9197)
                     /locus_tag="VFH05_10670"
     CDS             complement(8457..9197)
                     /locus_tag="VFH05_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6678726.1"
                     /translation="MKGSVKQDQLKIEQLCTRHRLLSRLSGTDRRRMARKMQVVSLSS
                     LHPLYEMSGVISNVFFPLSAVLFFLGTTLDGQVIEVGQIGSEGIAGVEVAMGTPYAGM
                     SLMTAAIQIPGLALQMNADDFAEEVDRYSAVNRCVRRYLGFFLAQLQFLIACNRHHNV
                     EQRGARWLLALQDRTGSRKLRITQQHLADMLGVTRQSAERLLNDFEVAKILDSQRGAV
                     TIKDRSKLHDRACACYRMLSKRLRALFV"
     gene            complement(9308..10033)
                     /locus_tag="VFH05_10675"
     CDS             complement(9308..10033)
                     /locus_tag="VFH05_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6678727.1"
                     /translation="MDELESFARKNRILNAIPTSELHRLLALMERVQLYPRQVLERPG
                     GGSEYIHFPIDGVLSMIASTREGDTVEVATVGNEGATGVIAMLSGSKQRAQQVPLVKV
                     RALIPGAVVRMRVDAFEAAIERSSEVNRCVMRYLNVLFSQLVLSASCNRLHSLEQRCA
                     RWLLGCRDRTQFEEVPVTQELLAEMLGVRRQSVDHVLGELETKQMIECLRGSVKIHDV
                     SSLRSVACECYPLAKERLDNSLT"
     gene            complement(10187..11017)
                     /gene="lgt"
                     /locus_tag="VFH05_10680"
     CDS             complement(10187..11017)
                     /gene="lgt"
                     /locus_tag="VFH05_10680"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET6678728.1"
                     /translation="MSWLQAIPYPHLSPVFFEIGPLQFRWYGLMYLIGLTAAYFLITR
                     KVREKGLAITKDQIYDMVVWAALGVFIGGRIGYTLFYNFSYYAQHPLKIFAVWEGGMS
                     FHGGLIGTIVSLIWFSKRQGIPVYTIADLAASVTPIGLGFGRLGNFINGELYGRPADV
                     PWCMVFPTGGPACRHPSQLYEAGLEGLLLFTVLWLIGRKVTPPGTVFWSFLTGYGLCR
                     IVVELFREPDAHIGFFFGSISMGQLLSLPMIVMGIFMLTLGYQRHAIAQRRNPSLKPQ
                     "
     gene            11345..11581
                     /locus_tag="VFH05_10685"
     CDS             11345..11581
                     /locus_tag="VFH05_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET6678729.1"
                     /translation="MSSEGKQVARWAALVAGSAAVGAGIGLLFAPQPGTETRREIARY
                     AKKAQLQATRLSRAVQSGVKEAMDRTKRPMVEAA"
     gene            11821..11909
                     /locus_tag="VFH05_10690"
     tRNA            11821..11909
                     /locus_tag="VFH05_10690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11855..11857,aa:Leu,seq:taa)
     gene            complement(11954..12328)
                     /locus_tag="VFH05_10695"
     CDS             complement(11954..12328)
                     /locus_tag="VFH05_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678730.1"
                     /translation="MAIGKQQACWATPTTTINCVLIRVDRQIEAEGCVLKVSESSSQL
                     AAPEFLAPGDFVKVRLWLEGENTSIHIGLAEVRRIQNHWITLEVVQVSRQERSRLQRY
                     VESRQTVLRNQPVFIDHLLIRA"
     gene            12425..12820
                     /locus_tag="VFH05_10700"
     CDS             12425..12820
                     /locus_tag="VFH05_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HET6678731.1"
                     /translation="MTSHRTSHRFFVLGAFFAGTAVAAGAFGAHLLKPVLDAPMLAAF
                     ETAARYQMYHALALCLISSAGGRYPNLNGTAVGWLFTAGILMFSGSLYALSLVGIPWL
                     GALTPFGGAAFMTGWALVAWGVWRSDRTL"
     gene            complement(12823..13734)
                     /locus_tag="VFH05_10705"
     CDS             complement(12823..13734)
                     /locus_tag="VFH05_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678732.1"
                     /translation="MLVSVTSRSLQALRALLMIACFVLVPTFLSAASEERASSSPPAW
                     HKFINLHHDRLAQVETDQAALDLFVAAIGRAAEVNDVAATIAAKGLSPQVTKDLLVPE
                     ITKSAQRLVAALVAWDIGDRMMRGLEEKSARTDVLTTPSQVAWLTANGPFTSLQEVVR
                     QWDSGDATAAAGVAAGQLAQDAAQRTMEEWWRLKTWKDRVRTARGQSRLCGTWLWVVH
                     NHQRHHEERKLSLVFPPPGMGTAIPGLTETVVLGDAVYLRWETDGRIQEDSLLFSKEG
                     QRLEGSFVNSQGGWGSITGKRTATCTP"
     gene            complement(13772..14569)
                     /locus_tag="VFH05_10710"
     CDS             complement(13772..14569)
                     /locus_tag="VFH05_10710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6678733.1"
                     /translation="MVSTTAPFTLQQIGTGTSRFPSGVALPTLTDASVDPYIRTRPIQ
                     TVQVECIEFWPQEKGTYPGIVLLHEWWGLNSQIKDLGARLACEGYGVIIPNLYGRIGG
                     MVTGNAEVAEALMGKLNDSMALQDINSCCEFLNTREFIKRNIHGVVGYGMGGTLAMKF
                     ACQRKRLRGAVSYYGRMIQPDSALENALCPILYHYAEQDPCVSGQDVQRLRELAAKYN
                     KPIAIESYSNAPHAFSDETRPESYREAAATEAWATTAMFLKKHLQGS"
     gene            14701..15699
                     /locus_tag="VFH05_10715"
     CDS             14701..15699
                     /locus_tag="VFH05_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6678734.1"
                     /translation="MHSSISSLSTAMLGIGLIGIGRHGSRYVKHLLRDLPDVALAAVC
                     RKSKSGVFSDATIPVYDDYRAMISDPRVHAVVVVTPPSLCYDICLAAVHARKPILIEK
                     PLALNGSQARTMVAAASQSGVLLMTAHTLRFDPVIKMLREQIHTIEPLQSACLTTHID
                     TKPNVMSVGDGSVKVGALLELGIHLLDLVRFLTKDNILEVQCTMSPLPSVAPETQVEV
                     RLRTSSGIACTLDIARVEVQRTGRAVWTGEKGTLTADWVARSVMRTAKDGRVEAWTLD
                     VKPTVLATLQAFVHAIRTATVPPITGLDGCLAVEAADACYRSAANHGAITLVGATR"
     gene            complement(15751..15987)
                     /locus_tag="VFH05_10720"
     CDS             complement(15751..15987)
                     /locus_tag="VFH05_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET6678735.1"
                     /translation="MATKAYILIKVKTGRTKDVLQALKKLAGVEQAHSCFGQPDIFLF
                     ISVQDERSLSDVVITKIHQIEGVEETDTHIVADS"
     gene            16716..18242
                     /locus_tag="VFH05_10725"
     CDS             16716..18242
                     /locus_tag="VFH05_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET6678736.1"
                     /translation="MLAKVLTVALTGLDAHLIDVEVDIAGGLPQFSVVGLPDATVRES
                     RDRVRSALKNTGFHFPAKKITVNLAPADIKKEGAGLDLAIAIGILVAEEVIAAEQLDH
                     RVLVGELSLDGQIKPVSGALSIALACRNHSLLLPAQNGPEAAMIDGVAVYGLHTLPEA
                     VAFLRGECKLEPHDADRTKWFSPAPADDEDYGDVRGQDHAKRALEIAAAGGHNVLMVG
                     PPGSGKTMLAKRLPTILPLMEPDEAIETTRVHSVASQLPADRPLLTVRPFRAPHHSIS
                     DAGLIGGGSIPRPGEVSLAHNGVLFLDESPEFRRPVLDGLRQPLEEGAVTLTRISGSL
                     RYPARFMLIAAMNPCPCGYYGDRSRECVCTPYQIRRYRARLSGPLQDRIDIHIEVPPV
                     PIRDLQDTNAAPEGSISIRTRVIEARKRQAGRYGSDGIHTNAQLKPRLVKRYCGLDTK
                     GQDLLEHSMMRLGLSARAHGRILRVARTIADLAGSDNIQAVHVAEAIQYRSLDRRVEF
                     "
     gene            18249..18464
                     /locus_tag="VFH05_10730"
     CDS             18249..18464
                     /locus_tag="VFH05_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678737.1"
                     /translation="MTAAMKVFVWWFLVGATMALAVIMLQGGMREVMQAQGSVWELKL
                     MELVTTIVGGGLLGGCVALILDRIKKS"
     gene            18499..18918
                     /locus_tag="VFH05_10735"
     CDS             18499..18918
                     /locus_tag="VFH05_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678738.1"
                     /translation="MDLEVGSNLYQNTDGTIEIEGVPQIQVALKPNALLINFAVFDAN
                     GKMTAKLVDSTLMFNERRAYEVTRAPTSVTLRHLESKNVVLQMDVKKPDVVTFTRGGF
                     HTVKGHLLEVSSTEWKVDKQRQSGTTQNAEGGPVKIG"
     gene            complement(18972..21569)
                     /gene="clpB"
                     /locus_tag="VFH05_10740"
     CDS             complement(18972..21569)
                     /gene="clpB"
                     /locus_tag="VFH05_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET6678739.1"
                     /translation="MDMNRMTIKLQEALQTASAHAMRRSHQGMDVEHLLLAFLEQEGG
                     IVPALCEQAGVSVPAVRQAAEQALSKLPQVQGTGGGPGQLHLTSRLNQVLTKAEDEQR
                     ALKDDYLSVEHVLLAMVHEGGIFKKLGLSKDKLLAALQQVRGHQRVTSQDPEGTYQSL
                     EKYGRDLTKLAGQGKLDPVIGRDDEIRRVVQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AIRIVKGDVPEGLKQKRVVGLDMGALVAGAKFRGEFEERLKAVLKEIQASQGQVLLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGELHLIGATTLDEYRKHIEKDAALERRFQT
                     VFVDQPSVENTISILRGLKERYEVHHGVRIKDAALVAAAKLSHRYIADRFLPDKAIDL
                     MDEAAARLRTEIDSLPADLDEVSRKVLQLEIEREALRKEKDPVSQARLTALEKELSER
                     QRDLQALKTRWDSEKASVARLRKTREAIEEVKLAIEKAERAYDLNRVAELRYGELPRL
                     ERELSIEQQQLGKKQGESRLVKEEVDEDDIAAIVSRWTGIPVSRLMEGETEKLLKLEE
                     LLHQRVVGQDEAVRAVADAVLRARSGIKDPNKPIGSFLFLGPTGVGKTELARALATTL
                     FDDETNMIRIDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPFSVILFDEIE
                     KAHHDVFNVLLQVLDDGRLTDSQGRTVDFKNTVLIMTSNIGSPQILEAQQKGSSYDDV
                     RAVVMGELRRHFRPEFLNRVDEIVVFHPLETDQLTKIVEIQLERLRGRLAERRITLTM
                     SPAALQHLAARGYDPVYGARPLKRLIQQYLETPMARQLVKGELRDGDTAMVDVKDDGI
                     VIVPTVTKS"
     gene            21689..>22163
                     /locus_tag="VFH05_10745"
     CDS             21689..>22163
                     /locus_tag="VFH05_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678740.1"
                     /translation="MINHLVGAFDLYRQALRQTWQSLQRGWITILAVIGFLFLLILAQ
                     QIAAPLGIAGGFLLGAVNALLVGVTLSLIEQSISYARTLTIKDVVESFGHYFWDVISV
                     GFVLWLPIMALDMGTRQNPNGPLISYAVMLLIFILLNPAPEIIYQVRHDSPLDVFK"
BASE COUNT         4596 a         6519 c         6304 g         4744 t
ORIGIN      
        1 agcaaaccgc ttggcccgag ggccgataaa tatcatggca aaggagcact catgactcgc
       61 acaacgatca tcaccgccgc cggggcattc atggtaccga gcttcatgtc tgcctgtaat
      121 cgtgaccaag accagacaac gccagtcgga caacaacaag gacaaggcag cgctcaggac
      181 tatccgtcca ctcgcaacat ggaacaaccc aatcctgcaa tcggcgggac caacagtgca
      241 acgaacaagc cggctccatc cggaaaccag gagccgcaag ctggttcaac tacgcggtag
      301 tgtcgttccc atcgcgcctt tctgcgtttc gaagctggtc gtgcctttgc cgcacgacca
      361 gcccgttcag cacagggctt cttgcaccgg cttttcccac ggtgtaagat ccaacgtctc
      421 gctcgtaacg tcctgagccc tatcaacgat gccgaacatc actttgttga tctctcccag
      481 ttgcggggcc tgtccgtcgg ccaagagcct atggaaacag ctccgggtca aatacagttt
      541 ttcataccgc gaagtggata ttacgacgcc agacggtcag gaactggcca atcggcatgc
      601 ggtgcgtgcc gtgccggcga cgatcatcga cgggcggctg acgttcgtcg gcgtgcccag
      661 ccgggaaagt gccgaaaagg ccctgcaact gaaaatgcgc ccgcgagaag cgtaggatga
      721 aatgttgttc gaactgacca tcccggaagg caggcgcgaa gcagagacgt aacctccgtc
      781 ggtcggtatg gtcatagatt ccaacggggt caagatggac gaagacgata ttgagcttcc
      841 gatcctgcct cgaatcgata aaggaccgcc tcctgactgt ccgatctgcg gcgaccccat
      901 gtccttcatc gacggtgatt gggcatgcgt tgactgtaat ggcgaattgc tgggtcccga
      961 gacaggatag tgcacggtgg ctagcgatcg gtggtgagtt gaccgaccac gcacccttgg
     1021 ggcactccca ctcacccccc tgccaccatc tcggtggctg aatgttgagc gtcgtcaccg
     1081 gccggtttca gcctgctctc gaatccgccc tcgtcaccca tatccaaggg atcaaagcgg
     1141 tcgacgtctg gtcgcagatt gccattgttg tgccatcgaa gccgttgctt gaccggatca
     1201 gatggctgtt ggccgttgag caccagctgg cgttcgtcaa cgtccacctt ttgaccttcc
     1261 atcagctcgc gctgcgctta gcggaggaga tccatcgcga aggcacaggg catccaccgc
     1321 gtgtcgtcga cgacctgttc tttgaatacc tcgttcgcca tcttgtccag cgacggctgg
     1381 cggagctgcc tgccctccgg caactcgggc gttcgttcgg gacgtgggga ggcctctggt
     1441 cgacggtgcg ggacttgaac gatggcgggg tcgatccggc caccgtactc cgcgcggtag
     1501 gggaaggtta tttcgatcca gacgaggcgg attggcttcg ggcgctgttt tcactgcacg
     1561 cggctgtaag ggaagtggga cgcggcttgc acttcgttac ggcggatgat ctgacggaag
     1621 ccgtgctccc tgcagtgtcc acttcaccat ttctgacgtc gttgagccac atcgcctatt
     1681 acgggttcta tgacttgacc caggtgcagc tttccatgtt tcaagcggtc agcggcaccg
     1741 tgtccaccac gctcttcttc cccctggcga cagattcttc attcgatttt gcccgacgct
     1801 ttttcgaccg tcatgtccag ccgctgctca aatcgaatga cgccctggtt gaggctgcgg
     1861 agactggcga agcgtttgcg cgcgtcgacg tcgcagtgcg aagcgtcgta ggccgggagg
     1921 aggaacttgc agttgcctgc cggaccatcc tcgatttggt cgaaacgcac gggtatcggt
     1981 tcgatgagat cggggtggtc gctcgaacac tcgatccata ccacagcgcc gcgtgcaaca
     2041 tcttcgatcg gcatcggatt ccattttcta caacggcgcg ccggccgctc agtcatgaac
     2101 ccctttgtaa aacgctgctt gccctggctt ccctgtcggc caatgattac taccgtgcgg
     2161 ccatgctcga tctcgtgacg tcaccatttt atcgggtgcc ccattcctca ggccggttcg
     2221 agcgtggtcc tgcatacagg cctgaacagt ggaagctggt tctcgaggcc ctgcatatca
     2281 cacacggcct tgatgagtgg acgcgtctaa agcaagccag tcaatcggca ctcgaatggg
     2341 aggtcgagga cgaggcagcc ggaaccattg gatcggtcaa gatcgccccg gacgtcatcg
     2401 gcctgctgtg ggagaccgtc tcggagctgc tgaagtctta cagtagtctt cctgcccgtg
     2461 ggacgatcgg ctcattgctt ccggcgttcc gagagctcat ggccaatcat gtgcatcgcg
     2521 tcgaagaaga gcacgcgatc tggagcgccg tcgatcaagc ttgcgcagcc gtcaacgaac
     2581 tggacgcgat cggtgaagac atcacctggg cggagttcgc ggaactgctc gcacatgcct
     2641 tcgaacgtgt gaccattccc cttgaggcca cgccccacca aggtgtgagg ctgttggatg
     2701 cgatgaccgc gcgaggtgtg cccttcaaag ccttgttcgt ccttggtctc aacgaaaaag
     2761 tctttcctcg acacgtgcgt gaagacgcat ttttgcgtga ccgtcatcga cgggtattgg
     2821 acgccacgct cggattcaag atcgatgaaa aactggcagg atacgacgag gaacgcctgc
     2881 tcttcagttt gatctgcggg tcggccgttc gtcggttata tctgtcgttc ccgcgtgccg
     2941 acgatgccgg acggatgttg gctgcgtctc cgtacatcgg aggtgaacta cgccgcctcg
     3001 gattcagaga ggtcgaagtg gaaatggttc ctcgtcgcct gtccgatcga atcaagcaac
     3061 ggccgacaat cagccgattc cttcccccgg aagatctggc tcagtggatg gcggggcggg
     3121 gggaagatcc cgccgagctt cttcaagcca caggccgcga cgccgctcta tatcaccgtg
     3181 gggtcgaggc gctggggctc attgaagacg atcgtgccgc attgacggca ttcgacggta
     3241 tgacaggccc actgaaaaac cactgggcgc gcatcgccga acgtgggatc gcgccaacgc
     3301 ctctcgaacg atatgcgagt tgccccttca agtacttttc agccgacgtg cttcgattgg
     3361 atccggtcag agtacctcct gcggatgagc cggacgcgcg gttgctcggc accgtatgcc
     3421 acgcggcttt gcgacgctgt tatgaattgc tgcttccaac agggtggccg gtgaaaccgg
     3481 tcactaacga caccatcgac tggtgcgtcg agacggcggt tgaacaggcg gccggagaac
     3541 aggaaatcct tcaccgaacc gggcactatc tcctgtggca actggcgaag agccagatca
     3601 ttgatgtcgt gacagccgct gttgatgacg atacacgcgc ctatcttgac gagcctttta
     3661 tacccatcct ttttgaagtt gatgccgacg gcagcatcga cgatctgccc ctgaatgtgc
     3721 cttcaccgtt acgtatgcgg ggccgcatcg accgagtcga tcgacatcgc atttccggtg
     3781 ctctgcgggt catcgattat aaattcaaaa tcggcaactc aatgaaaccg caagatcgta
     3841 accttttgct atcggcggtg agaggctacc gacttcagcc gcccatgtac gctcggttga
     3901 acgttccgga tcacggcagg gttcacgaag tccaactctt ctttttggca ccgcattggc
     3961 cgactcccgt cgtgcgctca acgtttaaga ccgatctctg gtcttctgac agcggcacct
     4021 cgctccgaac cacctttgct ctactggttg acggcctgcg gaacggcacc ttcttcatcc
     4081 tcccggacac gtattgcgag acatgtgact atcgcgtggc ctgccggcgt gagcatacgc
     4141 tgacgtcgtg gcgtgcatcg cgggccgccg aagcgaaggc gctgacattg ttacggacct
     4201 cgcgagtcaa cgatgcctaa ccgatcgagc ttgtctgata cggccgctcg agaagcggca
     4261 gaaacgacgt atgaccgcaa tgtggtcgtg gtggccggag caggaaccgg aaagaccact
     4321 ttgctcgtga accgtctggt gcatctgctg atgagggaac cggatccggt cggactgctt
     4381 caggtcgtcg ccctgaccta cacgaacaag gcagccacgg agatgaaaat ccgcctccgc
     4441 gagcggttga cgatgctggc tcaagtcccc cgcccgggag aaaccgggcc cgatggcggc
     4501 acgatggcca tcgaagatct gctgcttcgg tatggcatta cggttgaaga tattgcgggt
     4561 cgggcaacaa ccgccctgca ggatctggaa aaagcccaga ttggcactgt acatagtttt
     4621 gccggccacc tccttcgcct gtatccgttg gagagcggag tggaccctgc attccgcgag
     4681 gacgatggga cgcggttcaa tgaacatttt cgggtggcgt gggacgtctg gatcgaccgc
     4741 gagctgagcc ggcagggcac acagcacaag ctctggcgag cgctactcga atcgacgaca
     4801 gtcgcgcagc ttcgcacact gaccttctgt ctgtgcagtg atctgatcga tatcgaagcg
     4861 ctcacgcggc agcttgagat ggaatcgcct tccgaccttg cacagtggat cgggcgtatg
     4921 gctaccagat ctcaggcttt gcttgatgcc tatgaccggc ccaaacggcg caagatcgag
     4981 cacatgttgg cggccgcagc gacattgatg aagctcgttc gagacgaagg tccggacgcg
     5041 accacccagc ttgatgcggc cgaacgtgca tggcttgaga aaaaaaccag cgatgaggtc
     5101 agcggttggg aagaacatga cttcacagag gcaacgaaac tcattaagct ggctcagcaa
     5161 ttactgctgc ttgatcacca ctacttcaat aaattcttga agttagtttc gcccctcatc
     5221 acctccgtcc tggcgacctt tgcggacgcc ggctggctgt cctttgacgg gctcttggcg
     5281 cgcgcgcgaa ctctcttgtg ggagcatccc gccatccgtg aacgggtcaa acgggagtat
     5341 cgcgctgtct tgatcgatga gtttcaagac accgatcctc tccaatacga gattatcctg
     5401 gccgtgtccg aagagccgag acaccactca gtaaactggc agagcatcgt gctcgagccc
     5461 ggcaaactct tcatcgtcgg cgaccccaaa caatcgattt atgcctttcg ccgtgccgat
     5521 atggaagcct tcgatcgcgt cgtacagaaa atcgaagccg atggcgggat cgtccataac
     5581 ttgaccacaa actttcggag cgacgcgagc gtccttgaac cggtcaacga catattcgat
     5641 cgactcttcg tccggagacc gctggtgcaa cccggtaacg tccgcctcga aggcggcgag
     5701 aggcgtcgac gcagccgact tgaccctggc gtactcttgc agatcacggc atcgcgccct
     5761 ggcgatcctc cgttcgacgc ggccggagcg accagggcag aagccgaagc cctggcatgc
     5821 tggcttcggg aagaggtatt ggaaaaccgg caggtttccg caggtcagat tgcattactg
     5881 ttccgcacct tgacccaggc ggatgcctat ctggatgcgc tccgtcgcta cgacattccc
     5941 tatctcatcg aaggagaaaa gcacttttac cgccgccaag aagtgatcga cctcgtcaat
     6001 gtgctgcgtg tgctcgatca tcctcacgat cacatcgctc tggtcggtgt gctgcgttcg
     6061 ccgcttggtg gcttgacgga tcgcgacatc tacgacctgc acgaggccgg cttgttccat
     6121 tacttgaacg aggccggaac ggcgcagtgg tcccatccac gtgcagacaa cgtccgccgt
     6181 ctgtatcgtc ggttgacact cctgcatcaa caggtccgga ccctgccgct gccggaggcc
     6241 atccaagtaa ttttcgatga actaccagtg ctcgaattgg ccgcagcctc cttgcacggt
     6301 gagcaagcgg tagccaacct gctcaaggtc aaacagacgg cgacggcact cttggatagg
     6361 ccatatctga cattcagcgg attcatcgac ctgatggtga ctcggttgga tgagcaaccg
     6421 gaagaaccgg aaagcccgtt agccgaagaa tcgtctgatg ccgttcaaat cctgacgatc
     6481 cacaaggcca agggcctgga gttttccgtt gtcgtacttc ccggacttca ccaggggaaa
     6541 ggccgggagg gaacgtcacc gtcggtggtt tacgactggt cgacgggaaa ctacggcttg
     6601 tcactgggca ggctccatac aatgggctac ctgcgagttc aacaaaaact cgttgaacgg
     6661 caggaagccg agcggaagcg cgtctgttac gtgggcatga cgcgctcgaa ggatcttctg
     6721 ctcctctccg gcgccgtcgc ttcccgttcc gccggcgaca cggtgcttga ctggctgcac
     6781 gacatcggcg aaggcgaaat cggcaatccc atgaccgggg cgtggaaggt cggttccagt
     6841 gccattcccc atcgagtggt ctatgcccct gagcgaaaat cagggcggcg tcctttatcg
     6901 acgcatgaaa cagtgccgcc gctgcatcct gcgacgctcg cccaactgtg ggacgaacgc
     6961 actactcgcc gcggggcggt tcagggtatc tcatggcgcc tgactccgac gagcctgaac
     7021 gcgatcatgc cgacacatcg tgcggagagc ggtcgcacca ctgctgatcg cgacgtcggt
     7081 cggctggccg gtgtgttggc gcaccgtatc ctcgagggat gggatttttc acgatctccc
     7141 gtggaattgc tggatcagat tgcaccggct cttgataccg agctgactcc agaaaggctt
     7201 agaccggctg tcgcggcctc cctcaaagcg atcttcacgg ccttcggctg gtctgatgtc
     7261 tatatgcggc tgcgttcggc gacgatcctc ggccgagagg ttccgtttat catgccgtgg
     7321 ggcgatcggc aggtgatgga gggggtcatc gaccttattt accggcttga cggtaaactc
     7381 tggattgcgg actataagac agaccagacg acggctgagg aagccccgac caaagccgct
     7441 ttctattctt accaagcgga cgtttatcgg agagcggcaa cgaagtgcct gggtctgccg
     7501 cacgtgtctt ttcacctttt gtttcttcgg gcaggcacga gcgtggagct cacttgatcc
     7561 agcaaacggc ggcgcaacaa ccgcgaaaga ccgtcatcgt tgagtggctg attcaaacag
     7621 aggaggtgaa ccaatgcaca tcgtgtggag tgtcttgctc ctgtctgcga tgttcggatg
     7681 ttccggtcag tcgagcaagg ccataagcgt tcttgcagca cctgccggaa ccaaatcgac
     7741 cctcgccacg cagctcgagc agggaaacgc gctcttcgca tcgagcgact atgccggcgc
     7801 tgagcaggcc ttccggcaag ctcttgccat tgatgcgact tcagctgagg cccattacaa
     7861 tctggccgtc actctcgatc gaacgggaaa caaaacggaa gcgaaaagac attacattca
     7921 agccgccaat ttggctccgg ggaacaaggt gatctgggat tccccaccgc tccgtgcaca
     7981 aacgaacggt ctcaaccacg atatcagcag gaaatcgcac ttggacccgt cgaatcgtgg
     8041 tttctagaat ggcacggccg cgccgccgcg atgtgtctcc tgagcaattc gatctgctgg
     8101 tgcaacaagc gctggccgaa ttgcccgccc cctatgcgcg cctggctcag gaggtttcgg
     8161 tggttgtgga ggaagaaccg cctcccgacg tcttggagga tctggaattg gattctgccg
     8221 acgacctcct gggactgtac cagggtctcc ctgtggcagc ggagtctttt tttcatacgg
     8281 gcggccagca gcccccgcgc atcgccattt atcgcggacc gattttacgt ctctgcgaaa
     8341 ccgccgaaga cgtcagacgc gaaatccggg acacggtggt ccatgaattg ggccaccatg
     8401 tcgggctcgg cgatgatgac atgccgtatt agctcgccga caaacagatc gaacgcttac
     8461 acaaagagcg cgcgcagacg cttcgaaagc atgcggtagc aggcacatgc ccgatcgtgc
     8521 agtttcgacc gatccttgat ggtgactgcg ccacgttgac tgtccaggat cttggccact
     8581 tcgaaatcgt tcaggagccg ctcagcactt tgccgcgtga ctccaagcat atccgccaga
     8641 tgctgttgtg tgatccgcaa cttacgggac ccggtgcggt cttgcagagc cagcaaccat
     8701 cgcgcgcctc gttgctcgac attgtggtgt cggttacagg caataaggaa ctgtagctgg
     8761 gccaggaaaa agccgagata gcggcgtacg cagcggttga cagctgagta ccggtcgacc
     8821 tcttcggcaa aatcatccgc gttcatctgc agggccagcc cgggaatctg tatggccgcg
     8881 gtcatgagtg acatccctgc gtaaggcgtg cccatcgcca cttctacacc ggcaataccc
     8941 tcgctgccga tttgacccac ttcgatcacc tggccatcca atgtcgtacc gaggaaaaag
     9001 agcacggccg agagcgggaa gaacacatta ctaatgactc cgctcatctc gtagagggga
     9061 tgcagcgagc tgagtgagac gacctgcatt ttcctggcca tccttcttcg gtccgttccg
     9121 gacagtcgcg atagcagtcg atgcctggta cacagttgct cgatctttaa ttgatcctgc
     9181 ttgacgcttc ctttcacgga tcgctccttg cttttgatca tacgaaagga aacgcagggt
     9241 gcgaagagga aatacactgc aatccgtaga ggggatttac tgaagacgcc gtaagggaaa
     9301 ggaggagtca ggtcagggaa ttatccaggc gctccttcgc aaggggataa cattcgcacg
     9361 cgactgaacg taagctgctg acatcgtgaa ttttgacgga cccgcggagg cactcgatca
     9421 tctgtttcgt ctcgagttcg cccagtacat ggtccacact ctggcgtctg acgccgagca
     9481 tttctgccag cagctcttgc gtcaccggaa cctcttcgaa ctgcgtgcga tccctgcagc
     9541 ccagcagcca tcgcgcgcac cgctgctcga gcgaatgcag ccgattacag ctcgccgata
     9601 agacgagttg gctaaacagc acgttcaaat agcgcatcac gcatcggttc acttcactgc
     9661 ttcgttcaat ggccgcctca aaggcatcga ctctcatacg cacgaccgcg ccgggaatga
     9721 gcgcacggac tttgaccaat gggacctgct gcgcacgctg cttagagccg ctcaacatcg
     9781 cgatcactcc agtggctccc tcattgccga ccgttgcgac ttccacggta tcgccttcac
     9841 gcgttgaagc aatcatcgac aggactccat cgatgggaaa atggatatac tcacttcccc
     9901 caccgggccg ttccagaact tgccgcggat acagctgcac tcgttccatc aaggcaagca
     9961 gacggtgaag ctcggaggta ggaatggcgt tcaggattct gtttttcctg gcaaagctct
    10021 cgagttcgtc catggagcag cctcagtcgc ttctcagcgg cgcagtatcg ggcatcggcg
    10081 aagatgcagt ctgccaccta tatggcaatc ctgttgattt ccggtgcacg cgcgcaagag
    10141 ccccctggcg ggtgactcga cggtgggcag gaagctgcaa ccagacctat tggggcttga
    10201 gggaagggtt ccttcgttgg gctatcgcgt gtcgttggta cccgagggtg agcatgaaga
    10261 ttcccatcac aatcatgggt aacgacagca gctgtcccat ggagatcgac ccaaagaaga
    10321 acccgatatg agcgtccggc tcccggaaaa gttctacaac gatgcgacag aggccgtaac
    10381 cggtcagaaa gctccaaaac accgtgccag gcggggtgac tttgcgtccg atcaaccaga
    10441 gcacagtaaa caacagcagg ccttcaagac cggcttcata gagttgcgag gggtggcgac
    10501 aggccggtcc gccggtggga aacaccatac accagggaac atcggcgggg cgtccgtaca
    10561 gctctccgtt gatgaaattt ccaaggcggc caaatcccag gccgattggc gtgacggagg
    10621 ccgcgagatc ggcaatagta tagacgggaa tgccctgacg tttgctgaac cagatcagcg
    10681 agacgatggt cccgatcaac cctccatgaa acgacatgcc gccttcccac acggcgaaga
    10741 ttttcagggg atgctgcgcg tagtaggaaa aattatagaa cagggtatat ccgatccggc
    10801 cgccgataaa tacccccaac gccgcccaga cgaccatatc gtaaatctgg tctttcgtga
    10861 ttgccaatcc tttttcacgc actttgcgcg taatgaggaa atacgctgca gtcaatccga
    10921 tcaggtacat gaggccatac cagcggaact gcagtggacc aatttcgaaa aagaccgggc
    10981 tcagatgggg atagggaatg gcttgaagcc agctcattct tgtctcatgt ggcattctgg
    11041 ctggatcata agtgacacct aaaggcattg caagcctggc accgatgagg caagcgcctg
    11101 aagagaagcc ctggtcgcgg agttttcagc cttcctggct cgatgctgtt ggttttcccg
    11161 cacgactgtg tcgccctctg tgagaaaaag gccaccgaaa attagcaggc gttaatcggc
    11221 ttgaccgcct ttgaacgaat tatcggtcaa tccgtctacg gtacgactgg cacgcttgat
    11281 gctgaagcga atgaacgtga ggcgtacaac actcgatcaa cgttattttc caaaggaggt
    11341 tgccatgtct agtgaaggaa agcaggttgc tcgatgggcc gcactggtag ccggaagcgc
    11401 agccgtcggt gcaggaatcg gcttgctctt tgcgccccag ccaggaacgg agacacggcg
    11461 tgaaatcgcc cgctacgcca agaaagcgca gctgcaggcg acacggttga gccgggcggt
    11521 tcagtcggga gtgaaggaag caatggaccg cacgaagcgg cctatggtcg aagctgcgta
    11581 ggtcccctta tttttcaaat ccgcaaataa tcaccgacgc attcagtctg ttgccacagt
    11641 ggctgcaggc tgtttgcgtg tgttctgctc cttgaccgtc ctccacctcg atgctagagt
    11701 ccgcctgctt tctcattcag ttcggttagt tctaacagct ctccaggcca gctgtacctg
    11761 tccgctctcg gctcgacaga taccaggtga gcggaaggct ccgcgtgtac gactgaaact
    11821 gcaggagtgg cgaaactggc atacgcacag gacttaaaat cctgtggccc gaaagggccg
    11881 tacgggttcg atccccgtct cctgcaccag cgctcctgtg ttcagcgcgg ctcgtgtaat
    11941 ttttaaaggc ggatcaggcc cgaatgagga gatggtcaat aaagaccggt tgattgcgga
    12001 ggaccgtttg gcgtgattcg acgtatcgct gcaaccggct tcgttcttgc cggctcactt
    12061 gtaccacctc gagggtgatc caatggttct ggattcgcct cacctcggca agcccaatgt
    12121 ggatactggt gttttccccc tccaaccaca gacgcacttt gacaaagtcg ccaggagcca
    12181 ggaactcagg ggccgcgagc tgggaactgg actcggacac tttcagtaca cagccttcgg
    12241 cttcaatttg gcggtccaca cggatcagca cgcagttgat cgtcgtcgtg ggagtggccc
    12301 aacaagcctg ttgtttccct atagccatag gctgaggcta gcgtagagcc gatgaggcgt
    12361 ggttggctat ccctcaagag gaggggatag ccggaccttc atataatagg tcaggcgagt
    12421 gaacatgaca tcccaccgaa catcccaccg atttttcgtt ctgggagcgt tctttgcggg
    12481 cacggccgtc gcggctggtg cctttggcgc gcacctgttg aaacctgtcc tggacgcgcc
    12541 gatgttggcc gctttcgaaa ctgcagcacg ctaccagatg taccatgcct tggccctctg
    12601 cctcatctca tcggcaggcg gacgctaccc caacctaaat ggcacggcgg tagggtggct
    12661 tttcacggcc ggaattctga tgttttccgg aagtctgtac gctctctcgc tggttgggat
    12721 tccgtggctt ggtgcgctca ccccattcgg tggagccgcc tttatgacgg gctgggcctt
    12781 agtggcgtgg ggagtctgga gatccgatcg gactttgtag cgtcatggcg tgcaagtcgc
    12841 cgtgcgcttt cctgtaatgg aaccccaccc cccttgggaa ttgacgaaac tcccttccag
    12901 ccgctgacct tccttggaaa aaagcaggct gtcttcctga atccgcccgt ctgtttccca
    12961 ccgcagatag acggcatcac ccagtacgac cgtttcagtg agaccaggga tagccgtgcc
    13021 catcccgggt ggcgggaaca cgagcgacag cttacgctct tcgtgatgcc gctgatggtt
    13081 gtggacgacc caaagccatg tcccgcagag gcgagactgt ccccgcgccg tgcgcacgcg
    13141 atctttccac gttttaagcc gccaccactc ttccatcgtc cgttgagcgg cgtcttgcgc
    13201 cagctgtccg gccgcaacgc cggcggcggc cgtcgcgtcc ccgctatccc attgcctcac
    13261 cacctcctgc aaagatgtaa atgggccgtt tgccgtcagc catgcaactt gggatggcgt
    13321 cgtgaggaca tcggtacggg cgcttttctc ttccaaaccc cgcatcatcc ggtccccgat
    13381 gtcccaggca accaacgccg caaccaatcg ctgcgcggat ttcgtgattt ctggcaccag
    13441 cagatctttg gtgacttgtg gagagagtcc cttggcggcg atcgtcgccg ccacgtcgtt
    13501 cacctcagca gctcggccga tcgcagcgac gaacaggtcg agtgccgctt gatcagtttc
    13561 aacttgagca agccgatcat ggtgcaggtt gatgaacttg tgccaagccg gcggagacga
    13621 ggaagcgcgt tcctcagagg cagcggaaag gaaagtcggg acgaggacga agcacgcgat
    13681 catgagcaac gcccgtaagg cttgcagact gcgcgatgtg acggagacga gcatggtgac
    13741 ggacagatga ttcgcggcag agggacggtc gttatgatcc ttgaaggtgc tttttcaaga
    13801 acatggcggt tgtcgcccat gcttcagttg ccgcggcttc gcggtagctc tcaggtctcg
    13861 tttcgtcact gaaagcatgc ggcgcattgg agtaggactc aattgctatc ggcttgttgt
    13921 atttggcagc gagctcgcgc aaacgttgaa catcctgtcc tgacacacag ggatcctgtt
    13981 ccgcataatg atagagaatg ggacagagcg cgttctccaa agcggagtcc ggctgaatca
    14041 tgcgtccata ataagagacc gctccccgca ggcgcttccg ctgacacgca aatttcatcg
    14101 ccagcgtccc acccattccg tatcccacca caccgtgaat gttccgctta atgaactcac
    14161 gggtgttcag gaactcacaa cacgaattga tatcttgcaa agccatcgag tcgttcagtt
    14221 ttcccatcaa ggcctcggcc acctccgcat tgccggtcac cattccaccg atgcgaccgt
    14281 acagattggg aatgatcacg ccgtagccct cgcacgccag gcgggcgccc agatccttaa
    14341 tctgggaatt gagtccccac cattcgtgca ggagtacgat acccggatag gtgcctttct
    14401 cttgcggcca aaactcgatg cactcgacct ggacagtttg gatcggacgc gttctgatat
    14461 agggatcgac cgatgcatcg gtgagggtag gcagtgccac tccgctcgga aaccgagaag
    14521 ttcctgtgcc gatttgctgt aacgtaaacg gagcagtcgt cgaaaccata atccggcaaa
    14581 ctccctacca gcgtagcatc gcgcgataaa actctgcgaa aaattcatcg tactataggg
    14641 accgctccgc acgcttgtca atgtaacctc aaaggcagta gtctgcctca ggttttcgcc
    14701 atgcactctt caatcagttc tctctcgacg gcaatgctcg gcatcggcct gatcggcatc
    14761 ggccggcatg gcagccgtta tgtgaagcac ttgctccgcg atcttcctga cgttgccctc
    14821 gccgccgtgt gccgcaaaag caaaagcggt gtgttttctg acgccaccat tcccgtgtat
    14881 gacgattatc gagccatgat ttcggatcct cgcgtgcatg cggtcgttgt cgtgactcca
    14941 ccctctctct gctacgatat ttgcctggcg gcggttcacg cccgaaaacc gatcctgatt
    15001 gaaaagccgt tggcgctcaa tggatctcag gctcgcacga tggtggccgc ggccagtcaa
    15061 agcggggtac ttttgatgac ggctcacacg ttgcgatttg accccgtcat taagatgctg
    15121 cgagaacaaa ttcacaccat tgagccgctg cagtcggcct gtctcaccac gcatatcgac
    15181 acaaaaccca acgtcatgtc cgtgggcgat ggatcggtga aggtcggtgc actgcttgaa
    15241 ctcgggatcc atctgcttga cctcgtccgc tttctcacga aggacaatat tctcgaggtg
    15301 caatgcacga tgagcccctt gccctcagtg gcaccggaaa cccaggtgga ggtacggttg
    15361 cggacatcca gcggcattgc ctgcaccctc gacatcgccc gcgtcgaggt ccagcgcacg
    15421 ggaagggcgg tgtggaccgg agagaagggt acactcacgg cggattgggt ggcgcggagt
    15481 gtcatgagaa ctgcaaagga cggacgcgtg gaggcctgga cgcttgacgt caaaccaacc
    15541 gtcctggcga cgttgcaagc cttcgtgcac gccatacgga ctgcaactgt cccgccgatt
    15601 accggcctcg atggctgtct cgccgtcgaa gcggccgacg cctgttatcg ttcggcggcc
    15661 aatcatggcg ccatcacttt ggttggggca acccgctaga gacgcgtatc cgcccgtaat
    15721 cgaaatccgt cgccagcaat atctgcagcc ttagctgtct gccacgatgt gcgtatcagt
    15781 ttcctccacc ccctcgattt gatggatctt cgtgatgacc acatcggaga gtgaacgctc
    15841 atcctggacg ctgataaaga gaaaaatgtc cggctgcccg aagcaggagt gcgcctgttc
    15901 cactcccgca agtttcttca aggcctgcaa cacgtctttc gtccggcccg ttttcacttt
    15961 gatgagtatg tacgccttgg tcgccatggg gtctctcctt tcgatcaaag tgtttcccgc
    16021 ccggcatgct ccgtcggtgc tccgcgaaga tctgatggat atcgcgcctc tgctcgataa
    16081 acacggcacg cgccttggta tagagctcgc acagccattc caaatccgcc aatcgcatgt
    16141 tcacacgagc gaaaggtgct gctaacctgt cctgctcttc tctcgaaatt gtggcataac
    16201 gggcgcatat cgcttccagg acctgagaag tccaccctcc ttgacggaat tccgtttccg
    16261 cttcctccgt gcgatcggca aacttcgacg ccaacgcgtg atccagaaat tgccgtacgg
    16321 cggaatcggt gctgttcatg aatacacctt gaaactgaat cacgatctga atcacacgat
    16381 tcgcccgcgg agtcccttca gggggaagaa tgctggcatc ttgcagcgtg gctaaaagcg
    16441 ccatcgaggc tcccaccggt ccggatggag tccggatctg ccccggggcc atcggtgaca
    16501 cttccgcttg atgcagcaag acgagcgaca agacgatgcc cggccaacgc atgccgcgga
    16561 ttataccgaa gggacctcat gtgcaccagc ttgcatcaac agcatgaaac gggttggcca
    16621 atggaagcat ttcccccttg ctggacgtgc tttttcttga atccagcaca gacgctttct
    16681 ataattcagc gccccagtga ctgcatggag ttttcatgct ggcgaaagtg ctcaccgtcg
    16741 cgctgaccgg actcgacgca catttgatag acgttgaagt agatattgcc ggcgggctgc
    16801 cgcagttttc cgttgtcggg ctgccggatg ccaccgtccg tgagagccgc gaccgagtcc
    16861 ggtccgcact caagaacaca ggctttcact tccccgcaaa aaaaatcaca gtcaatctcg
    16921 cgcctgccga catcaagaaa gaaggggcag gcctcgattt agcaatcgcg atcggcattc
    16981 tggtcgcaga agaagtcatt gccgctgagc agctcgacca tcgcgtgctc gtcggggaat
    17041 tgtctctcga cggacagatc aaaccggtgt ccggagccct ttcaatcgct ctggcctgtc
    17101 gcaaccacag tctcttgtta ccggcacaga atggacccga agccgcgatg atcgatggag
    17161 tggctgtgta cggtctgcac acgctgcctg aagcggtcgc attcctcagg ggtgagtgta
    17221 agctcgaacc gcatgacgcc gaccggacga aatggttctc ccccgcgcct gcggacgacg
    17281 aggactacgg cgatgtccga ggacaggatc acgccaagcg cgcgttggaa atcgccgctg
    17341 cgggcgggca caacgttctc atggtgggtc cgccaggctc gggcaaaacc atgttggcca
    17401 aacgtctgcc gactattctt cctctcatgg aacccgatga agcgattgaa accactcgtg
    17461 tccacagcgt ggcttcccag ctccccgcgg atcgtccact gctgaccgtc cggccctttc
    17521 gcgcgccgca ccatagcatt tcggacgccg gcttgatcgg cggcggaagc atcccccggc
    17581 cgggtgaggt ctccctcgct cataacgggg tcctgttcct ggatgaatcg cccgaattcc
    17641 ggcgaccggt ccttgacggc cttcggcagc ccctggaaga aggcgctgtc acgttgacga
    17701 ggatcagcgg ctcgctccga tatccggctc gattcatgtt gattgcggca atgaaccctt
    17761 gtccctgcgg gtattatggg gaccgctcac gggaatgtgt ctgcacgccc tatcaaatcc
    17821 ggagataccg cgcacggctc tcaggcccgc tgcaggaccg tatcgacatt cacatcgagg
    17881 ttccaccggt gccgattcgc gatctgcaag acaccaacgc agcacccgag ggctcgatca
    17941 gcatacgcac gagggtcatt gaagcgcgca aacggcaggc tggccgctat ggatcggacg
    18001 ggattcacac aaatgcccag ttgaagcctc gactcgtgaa gcggtattgt gggctcgata
    18061 cgaaagggca ggatcttctg gaacacagca tgatgagact gggcctctcg gcgcgcgcgc
    18121 atggtcggat cctcagggtc gcgagaacca ttgcggattt ggcaggctca gacaacattc
    18181 aagccgttca cgtggccgaa gccattcagt accgcagctt ggaccggcgt gtggagttct
    18241 agaatcctgt gaccgccgct atgaaagtgt tcgtctggtg gtttctcgtc ggagcgacaa
    18301 tggcgctcgc ggtcatcatg ttgcaaggcg gcatgcggga agtgatgcag gcgcaaggct
    18361 ccgtctggga gttgaagctc atggaactgg tgacgaccat cgtaggaggc ggtcttctcg
    18421 gcggctgcgt tgccctcatc ctggatcgga tcaaaaaatc gtagcctcca cctgacggca
    18481 ggggacaaag gagtgacaat ggatctggag gtcgggtcga atctgtacca gaacacggac
    18541 gggacgattg aaatcgaggg cgtcccgcaa attcaagtgg cgctgaagcc gaacgccctg
    18601 ctgataaatt ttgccgtatt cgatgccaac ggcaagatga cggcgaagct ggtggacagc
    18661 accctcatgt tcaatgagcg cagggcctat gaagtcacaa gagccccgac cagcgtgacg
    18721 ctcaggcacc tggagtcaaa aaacgtggtg ctgcaaatgg acgtcaagaa gcctgacgtt
    18781 gtcaccttta cgcgaggcgg atttcatacc gtcaagggtc atctgctcga agtgtcttcg
    18841 acggagtgga aggtggacaa gcagcgtcaa agcgggacca cgcagaatgc agaaggtggt
    18901 ccggtaaaaa tcggatagct accctgtgag actgggtcag ggcgcgccgg gccgggtggg
    18961 atgaaattga atcaggactt agtcaccgtc ggcacgatga cgataccgtc gtccttcacg
    19021 tccaccatcg cggtatcgcc gtcccgtaac tcgcctttca ccagttgtct ggccatcggc
    19081 gtctcgagat actgctgaat cagccgcttt aacggccggg cgccgtacac cggatcatat
    19141 ccgcgtgctg ccagatgttg aagcgccgcg ggcgacatcg tgagcgtgat gcgtcgttcg
    19201 gccagtcgcc cccgcaaccg ctccagttgg atttccacga tcttagtcaa ctggtccgtc
    19261 tccaggggat ggaatacgac gatctcgtcc acgcggttca agaactcggg acgaaaatgc
    19321 cgccgtaatt cgcccatgac tacggcgcgg acatcgtcgt aactggaacc tttctgctgc
    19381 gcctcgagaa tttggggact gccgatgttg gacgtcatga tcaacaccgt gtttttgaag
    19441 tccacggtcc gtccttgaga atcggtaagg cgcccgtcgt ccaagacctg aagcagcacg
    19501 ttgaagacgt cgtgatgggc cttctcgatt tcatcgaaga ggataacgga gaaagggcgt
    19561 cgccgcaccg cttcggtcaa ctgcccgcct tcctcgtaac cgacatagcc gggcggagct
    19621 ccaatgagcc gggcaaccgt atgcttctcc atgtactccg acatatcgat acgaatcatg
    19681 ttggtttcgt catcgaacag cgtggtagcg agcgctctcg ccaattccgt tttaccgacg
    19741 cccgtcggcc ccagaaagag aaaggacccg attggcttgt tcggatcctt gatgcctgat
    19801 cgcgcgcgca ggaccgcgtc ggccacggcc cgaaccgcct cgtcctgacc gacgacccgt
    19861 tgatgaagca actcttctaa cttgagaagt ttttccgtct cgccctccat caaccgcgag
    19921 acggggatgc cagtccaacg gctcacgatg gccgcgatat cgtcttcatc cacctcttcc
    19981 ttgaccaggc ggctttctcc ctgttttttc cccagctgct gttgctcgat cgataattca
    20041 cgttcgagac ggggcagttc accgtaccga agctcggcca ctcggttgag atcgtatgcg
    20101 cgctctgctt tctcgatcgc cagcttcacc tcttcgatgg cttcacgagt ttttctgagc
    20161 cgtgccaccg aggctttctc ggaatcccat ctggtcttca gggcttgcag atcgcgttgc
    20221 cgctcactca gctctttctc aagggccgtc aaccgcgcct gactgaccgg atctttttcc
    20281 ttgcgtaatg cttcgcgctc gatttcgagc tgcagcacct tgcgggacac ttcgtcgaga
    20341 tcggcgggga ggctgtcgat ctccgtccgg aggcgcgccg cagcttcatc cataagatcg
    20401 atcgctttgt caggcaggaa ccgatcggca atgtagcgat gcgagagctt cgccgccgcc
    20461 acaagcgcag catccttgat ccgcacgcca tgatgcactt cgtaccgttc cttcaggcct
    20521 cgcaggatgg aaatcgtatt ttcgacggag ggttgatcca cgaacacggt ttgaaaccgc
    20581 cgctccagcg ccgcatcttt ttcgatatgt ttgcgatact cgtccagcgt cgtcgcgccg
    20641 atcagatgca attcgccgcg ggccagcatg ggcttgagca ggttggcggc atccatggcg
    20701 ccctctgcgg ctcccgcgcc gacgaccgtg tgcaattcat cgatgaacag cagcacctgg
    20761 ccctgcgaag cctgaatctc tttcaagacg gccttcaacc gctcttcaaa ctcgccgcgg
    20821 aatttcgccc cggcgaccaa ggcgcccatg tcgagaccga caacgcgttt ctgcttcaat
    20881 ccttccggta cgtctccctt cacgatccgg atggcaagcc cttcgacgat ggctgttttg
    20941 ccgacgccgg gctctccaat gaggacggga ttattttttg tgcgacggga cagaatctgg
    21001 acgacacggc gaatctcgtc gtcgcgcccg atcacgggat ccagtttgcc ctggccggcc
    21061 agtttggtca ggtcgcgtcc gtacttctcg agcgattgat acgttccttc aggatcctga
    21121 ctggtgacgc gctgatggcc acggacctgc tgcaaggctg caaggagctt gtctttggac
    21181 aatccgagtt tcttgaaaat gccgccttca tgcaccatgg caagcagcac atgctccaca
    21241 ctgagatagt cgtctttcaa cgcgcgctgc tcgtcttccg cttttgtgag gacctgattg
    21301 agccgcgagg tgaggtgaag ctgccccggg ccgccgccag taccttgcac ctgtggcaat
    21361 ttgctcaagg cttgttccgc cgcctggcgg acagcaggga ccgaaacccc ggcttgctcg
    21421 caaagggctg gaacgatgcc gccctcctgt tcgagaaacg ccaggagcag atgctcgaca
    21481 tccatgcctt ggtgactgcg gcgcatggcg tgggctgatg cagtctgtag ggcttcctgc
    21541 agcttgatgg tcatccgatt catatccatg atggtggctc cagattcagt ctattcactc
    21601 gtataccgac ataatcacag ggagtcgaaa gtcaacttgg gacggagact gccttgaccg
    21661 gccgcgaggg accagactat tgtcattcat gatcaatcac cttgtaggag ctttcgatct
    21721 ttatcgccag gcgttgcgtc agacatggca atccctgcag cgaggctgga tcactatctt
    21781 agcagtcatc ggattcttat ttctcctcat cttggcccag caaatcgcag ctccgctggg
    21841 aattgcaggc ggttttctgc tgggcgccgt caacgcctta ctcgtggggg tgacattaag
    21901 tttgattgag caatcgattt cctatgctcg gacattgacg atcaaagacg tcgtcgagag
    21961 ttttggtcac tacttttggg atgtcattag cgtgggcttc gtcctctggc ttcccatcat
    22021 ggcgctcgat atgggcacga ggcagaatcc gaacggtccc ctgatttcct acgccgtgat
    22081 gctgttgata ttcattctat tgaatcctgc tcctgaaatc atttatcagg tgcgtcatga
    22141 ttccccgctg gatgtgttca agc
//
LOCUS       DASSCQ010000155        16378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_241083, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000155 DASSCQ010000000
VERSION     DASSCQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 16378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16378
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241083"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFH05_10750"
     CDS             complement(<1..505)
                     /locus_tag="VFH05_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678741.1"
                     /translation="MTTKNGIESGLKKRVLLVDDEARVRASLKAVLEPNYEVIQAADA
                     QEGLDLFHKEAPHVVLLDVILPGTDGLSVLQTLRTEQNTTPVIMLTGTKSVKTAVDAM
                     KLGAADYLSKPFDVEELRIVVDRALNSHELEREVKQLRAQVTQRYAFHNLIGKSRPMQ
                     EIYGKIEQ"
     gene            complement(502..1767)
                     /locus_tag="VFH05_10755"
     CDS             complement(502..1767)
                     /locus_tag="VFH05_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6678742.1"
                     /translation="MSARPTEMGSDATPSSIELLDSVTRFSHEIGTLSDLEALGERII
                     RELCRVSSTRHGALYVLDREHEHYRRACIVGVTAESCCPVILALSDTIPQHLFSAPQT
                     VTRSCCVPIRRGDASNEAGMMPFEWALPHISRGRLIAFSLFGRSLSQDSDSPSTVSLL
                     NALAQAATNALDTRMLLEDLQRSHLLMKRTDRLKSLETIAGGFAHEIRNPLTSIKTFI
                     QLAPERKDDPDFIREFSRVVLDDVYRIERLIEEILDYARYMEPKLTEEDPNDIVSSCL
                     YFIEVKADSRGIKIEKELVSDLHRVMLDRQQIKQVFLNLFLNALDAMKEGGGVLRVRT
                     RNLIKPGGTSWVQIETQDTGHGIQEANLEHIFDPFFTTKHESGEHEGTGLGLTIVHQI
                     IEEHHGEIQVTSEVGSGTTFLVNLPAIPS"
     gene            complement(1877..6052)
                     /locus_tag="VFH05_10760"
     CDS             complement(1877..6052)
                     /locus_tag="VFH05_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678743.1"
                     /translation="MPQSEPITILLINEHAEEIKVVTISLRGFFHDVRIDAAYSTEEA
                     SRMASATTSGWAVVLIDEGCLPSGRSTLVHELKRQIPHASVILQSTRTDAAAALEALE
                     NGADFFLNKQSPGFLTELLFCAKEAFDKRDLRVAADRAQVRHSWLVDSLTDIVYELDA
                     DGRFLTLGRSLSALLGYHPDELIGRPYDTLFPQDQQPLARFRFNERRTGIRASTGVEL
                     TLLGKPTAAGVSQVTAAVSARGVYDLSRRFVGTVGLIRQVPEQKKGQPRPREIAPQQR
                     TDDVRALVRQMGDLAQRLQHPLSTLLQEAQQLSDRLRASGVIEGLGALIEHAAASAQL
                     GEQLSRAAEEVPRADTINRLLEDLLTSAFSDAAAGVVVSDLTSALPVYEGDREKTTLL
                     LRRLLTYAGGFIAAVGRSHGLAVKTSVDTPPGFADAPTLFPLTQSRQVQVDITETEHT
                     VAAAAWPTRHSPLDAAELFQLAGELGAALDISAPAQGPFRITVRFPAISFPSLPQPSA
                     PPEPDVPLAAHPPAVTPPPPPRDIRRATAARTDRRSYDRVPTAMQAKVHSGSSEWDGT
                     VVNMSVGGACIQMPEDFPSIALQEAQVRVTTSVATLELHGLIYERVVPESIAQPSPVS
                     PSHLIVALNRTPQIEAAVLASLITATHERSLLFTLDIRLAPRRRTGTLEPGKDVTLDQ
                     IDDDRREAVRITLGLPARLEASRRPEPAGHIPAQVTNLSRSGAALLIQARPEDLQGSL
                     RIHFASAHRGDGLETGAPDTILPAHVIWSAPDRTASTALHVPGTDFAARAGLRFDPLT
                     PYAERELNRVIRQYLRAQRASEPSSAAPIVSIPRECRNARGQAISIMDDHHLQLTDAS
                     RPVIIIAPGYGQVALDYAALAYYLAAHQFRVLRYDPTNHLGNSDGELQHTSLRSLQHD
                     LDKVIEFVRDTWPQAPVLVIASDLGARAALKSAAQIRPLDLLLLVNPAVDVRHLLMTA
                     HGHDLLADYQFGLRRGVCNVLGLNVNVDQFVGDLIGARCADLDSTLEDLRLIRSPLCI
                     ATSPAVSAALPPADLPHAFMTALGAHTKLVNLSTPLTQRRLDVHGTLPAAFKQLLMHI
                     GSVISVQGLSAQQDISAHPFIARQRRIEEEFTFLRHEGSQINREALCAAHLTQLPQLG
                     NLHEYRKLLDDLYGLMSPLDSGSVVVDAGVGHSDLTRAALVNHTYRAGQASPLGRRPP
                     LMIGVGRARERLVQARHAVLLLQRELASGFAGRLSAMPPLAIGWNQADWMSSLPFKTG
                     SVTRLVCNLSLAYVPSPAVALQEWHRTLHPEGSLIVTTFHPDTDLSPLYRRHLRQANQ
                     DEFSAQAQPLLHYFARLREAIRHRLLHTFDEAGLASLLRHCGIESFRILPIFDGQALV
                     AVVGKQNSSSSIR"
     gene            complement(6344..7336)
                     /locus_tag="VFH05_10765"
     CDS             complement(6344..7336)
                     /locus_tag="VFH05_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678744.1"
                     /translation="MFVLLAVVLCLWSAPVMADGGHDHHAVPTLKNQTTTVVAITDNA
                     VTLTFGPIDLPSGHEGDLAASMPKHVFQLPKDMYMVGFKSAVFTKDGKPLPKNYLHHI
                     LMLNNDKQSVSCPGEPLFFSGAGLEMTEARFPDGYGVKLGKGQRLMSVVAFYHKAPPT
                     KNVMASFTMYMAPENRPVKEMEVYQVGVNVVCYSKFAQRGPDQTDEGIEIKPGVQVHS
                     APLKFSMDGCVKFAYPHGHDELLLIALENKTRQQTLLRTVPDVTLDGTFLEFQPHQVY
                     KDPQGFSVSKEEDYEMIMVHHHPLQKQDPQHGMGNYLLYMTPGTCPTQKTASAH"
     gene            complement(7423..8286)
                     /locus_tag="VFH05_10770"
     CDS             complement(7423..8286)
                     /locus_tag="VFH05_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6678745.1"
                     /translation="MNRGPCVWVLSAIVMLASVSSGRAADDQSPEATVRALVRANAEK
                     DMETMSRLIAHDADITSYTIGGRKYVGWGDLERDMEEEFKSAATIEIPIRELKVWTRG
                     DLAWYAMEVDYIRTVTTGSQPQRALLPLRETGVLERRDGHWVLLSWHESFRTAGGAVA
                     LNEHASVTLASSNNHLAQTDLSGEWEIWEVEDDKRYRATLDKAGNGPYTQHGGRFVTS
                     RFSDRLWQGTWQQPGNDREGGFEILLSEDGKEAKGVWWYTRVGTHKNIPPREHGGTYQ
                     WKRITPVPTAP"
     gene            complement(8357..9166)
                     /locus_tag="VFH05_10775"
     CDS             complement(8357..9166)
                     /locus_tag="VFH05_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET6678746.1"
                     /translation="MKQSPRWLGVGRTETGHVRKTNQDVFAAINSCNFWIIADGMGGH
                     PAGDVAAHVAVDVATQTAKTLLSTREQSSQPPDQSLIETVIAANKAIHEKIRENSSLK
                     GMGTTVVAMTIPPGPTPVAHVAHLGDSRAYRFHAGVLTQITRDHTLVEVLLQRGLINE
                     ATARAYPDRHVLAKGLGMGLEMTPDLTSTPLAPDDTLLLCSDGLTKMLSDAQIGSILA
                     TTHANPRRACHELVEQALRAGGEDNVTVIVCAYTTPGKVGSLAIDKTPPAM"
     gene            complement(9171..9968)
                     /locus_tag="VFH05_10780"
     CDS             complement(9171..9968)
                     /locus_tag="VFH05_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET6678747.1"
                     /translation="MLTSTFVLLKGIGPATERRLWQDGVADWSAFLGRALITGISATR
                     KGWYDEVLATAQSRLAGGSADFFRTCLKSRDHWRLFHTFRHRATYLDIETTGQSSGQA
                     VITVVGLYRNGRMTSLVRGENLTEDRLAEALEGTDLLVTFFGSAFDIPFLQQAFPRLN
                     LQKPHFDLCFAARRLGLTGGLKYIEREMHIDREPEIQGLDGWDAVRLWYQWRGGDEGA
                     LDLLLRYNAADTRNLEPLAEVLYEQMVSRFGPPSIGPVLAQVKPAGQ"
     gene            10073..10456
                     /locus_tag="VFH05_10785"
     CDS             10073..10456
                     /locus_tag="VFH05_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HET6678748.1"
                     /translation="MADPGNKIRIIVGGVQVEAELKGTKTAREVYAALPVEAPVNTWG
                     EEFYFKLDGVRDYRETATNQVKVGDVAFWGAGQVLAIFFGRTPMSMGPDPVPADRVNV
                     IGRILGDPTVLRSVMAAPSIRVERV"
     gene            10460..10741
                     /locus_tag="VFH05_10790"
     CDS             10460..10741
                     /locus_tag="VFH05_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HET6678749.1"
                     /translation="MRLSKERVRHMAETLAGRLVQESHVALTGTQKAFIESLDQVVTD
                     ELSIEDRLNSEVRQLLKAYERQIETGQVDYQKMFTMIKQKLVRERGIIL"
     gene            10801..11073
                     /locus_tag="VFH05_10795"
     CDS             10801..11073
                     /locus_tag="VFH05_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HET6678750.1"
                     /translation="MLSEDKVSHLSHVILLAVKNSPHVEVKSEDARILKDIKRVLATE
                     LAQEQEIDRKVRAKLGSYTRGIVEGSPEWDVLYRKTFEEESRKHTK"
     gene            11099..11539
                     /locus_tag="VFH05_10800"
     CDS             11099..11539
                     /locus_tag="VFH05_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678751.1"
                     /translation="MKFGFPVLVALVLIVSLGACTKGKARPLVPLALEPHAKAPAVAL
                     TEQGMQAYQTRKFDEAKRYFSQAVTEAPQSGPAHYNYALALNALGDTEEARQHFIEAA
                     NLAPGDKTIWDSPALHQYGNPQTEKETKEHRTTTSRPTFGGPPR"
     gene            11554..12021
                     /gene="bcp"
                     /locus_tag="VFH05_10805"
     CDS             11554..12021
                     /gene="bcp"
                     /locus_tag="VFH05_10805"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HET6678752.1"
                     /translation="MPKDIAVGDKAPDWSLPDHTGKPVTLKSLKGKQIVLYFYPKDDT
                     PGCTKESCDFRDVESQIIRAGGVILGVSLDDKESHQKFVKKFGLPFPLLSDEDAAVSK
                     AYGVYKEKNMYGKKYWGIERSTFVIDPDGKLKAVFRKVKVDGHVDEVLTALRS"
     gene            12147..12932
                     /locus_tag="VFH05_10810"
     CDS             12147..12932
                     /locus_tag="VFH05_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678753.1"
                     /translation="MEKITPTVLVKDSLSAPHQQTTIEAKLFAKGLLKDSPLGGEPVE
                     LLVKGIVSATAMTGGDGRAFLSFIPKGKDILPIQVRTGSSPRVSSGEGEAHLVVWERR
                     TPILIVELTALMEDVHAETPVSRLVPRIDPEARPIADAADELGKLTQFYYGVIYVVTV
                     PAGADVFVTSVEARTWLSTHKFPRGFVLALSGGEDALGAKIDELHQAGWKSAKTGIGR
                     SKAFAETFLKRRLEAIIVPEPPVGEVPRKGKVAKDWKDVRKKL"
     gene            13044..14405
                     /gene="radA"
                     /locus_tag="VFH05_10815"
     CDS             13044..14405
                     /gene="radA"
                     /locus_tag="VFH05_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET6678754.1"
                     /translation="MKSKSIFSCQACGHQSPRWLGRCPDCGGWNTIKEERHAPTGKGR
                     PVAMNLAQPTATPIAKIEVIGEDRRLTRISEFDRVLGGGVIPGAVILIGGDPGIGKTT
                     LLLQALPRLASNVQPVLYVSGEESPRQIKMRGQRLGVDHPNLLVLAETSLEQILKAVQ
                     DIQPAAVVVDSIQTVYTEQLTSAPGSISQVQEVSGQLMWFAKRSGVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKGHSYRILRAVKNRFGSTNEIGVFEMKESGLEEVTNP
                     SQLFLGERPQRSTGSVVVSSVEGSRPILVELQALVSSTSYAMPKRMTNGVDLNRVSLL
                     LAVMEKRLGMHLSGQDVYVNVVGGMHIDEPAIDLGIVAAVTSSLREVPVEPGLLVLGE
                     VGLGGEVRGISQAELRIREAAKMGFKRCLLPERNLAKLEPVDGMELVGIHEVGEALDV
                     VLA"
     gene            14368..15291
                     /locus_tag="VFH05_10820"
     CDS             14368..15291
                     /locus_tag="VFH05_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678755.1"
                     /translation="MKSEKRWMSYWRKSALCLLPYLTVLCFVAAGCTTPVQREEADTK
                     AATADELTALLEQRERAVQTMKGLFSAKVRGGIIPITTRVEGAVYYRRPSAMRLRGFT
                     AIGSELFEFLQTDDQFTLRLPTMGRVVSGHPSDMSEMGKLARPFQLSVWAMSGVLGTG
                     TINAGDTVRVVDEGDRHRLEVFRASPSGGLTIHRRLWFNRETRLVVKEDRLTELGEID
                     ATIVYEDFRPVGEMTGLSISGDGQLLRPFKISLEDGQGRGSVQVTFHELIPNATLKPS
                     ELAHASNALPDTGRLATGTDGARQLPNQDLP"
     gene            15349..16131
                     /gene="tsaB"
                     /locus_tag="VFH05_10825"
     CDS             15349..16131
                     /gene="tsaB"
                     /locus_tag="VFH05_10825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET6678756.1"
                     /translation="MKVLAVETATSWQSVAILDDDRVLARCDQDAAGAHARLLLPAVD
                     KLFSQTGLGPAKLDGLVVSIGPGSFTGLRVGLSTVLGFRTITRVPLAVVPTLEGMAWS
                     LRASNERLCPVLNSRRGELYWAIFQWQGRDRLDRLTPEQVGSVETLAKQLSGSVMMFG
                     EGWETERAAIQAMGRSVKIMEADQLLRPSAVSIGMAGIARLRRGEQAGVGISPLYVQR
                     PEAEIKYEESGGISPMSRRQQKLARKLRNRPPLSATKTRRQT"
     gene            16128..>16378
                     /locus_tag="VFH05_10830"
     CDS             16128..>16378
                     /locus_tag="VFH05_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678757.1"
                     /translation="MMPPDWTIGAANLESLSEILAVEEACFSAPWTRKMLEGELSGNP
                     FAHFLVARPRRVEEASKQAILGYLCYWIVFEEVRLMNLAV"
BASE COUNT         3452 a         4490 c         5032 g         3404 t
ORIGIN      
        1 cctgttcgat tttgccgtaa atttcctgca tcggacggct ctttccgatc aggttgtgaa
       61 acgcgtaccg ctgggtgacc tgtgctcgaa gctgtttgac ctcgcgttcg agttcgtgag
      121 agttgagcgc gcgatccacg acaatacgca gctcttcgac gtcgaacggc ttcgagaggt
      181 aatcggcggc gccgagtttc atcgcatcga ccgccgtctt gacggatttt gttccggtta
      241 gcatgatcac cggcgtggtg ttctgctccg ttcgcagggt ctgcaacacc gacaatccgt
      301 ccgtccctgg aagaatcacg tcgagcagca caacatgcgg cgcttctttg tggaacaagt
      361 cgagcccttc ctgggcatcg gccgcctgga tcacctcata attcggctcg agcaccgctt
      421 tcagcgacgc ccggacacgc gcttcgtcat cgaccaatag cacgcgcttt ttgaggcccg
      481 attcgatacc gttttttgtc gtcatgatgg aatggccgga aggttaacaa gaaatgtcgt
      541 gccgctgccc acctcactcg ttacttgaat ttcgccgtga tgttcctcga tgatctggtg
      601 cacgatcgtc aggccgaggc cggtcccttc atgttcaccg ctctcgtgct tcgtcgtgaa
      661 gaacggatcg aaaatgtgtt ccagattggc ctcctgaatc ccatgacccg tgtcctgggt
      721 ctcgatctgc acccacgacg ttccgcctgg cttgatcaga ttcctcgtcc gaacgcgcag
      781 caccccgccg ccttccttca tcgcatccaa ggcgttcagg aacagattga gaaacacctg
      841 tttgatttgc tgacggtcca gcatgacacg atggagatcg gacaccaact ctttttcgat
      901 cttgatcccg cggctgtccg ctttgacttc aatgaaatac aggcaggagg agacgatgtc
      961 gttcgggtcc tcttcggtca gctttggttc catgtacctg gcataatcca aaatttcctc
     1021 gataagccgt tcgatgcgat agacatcgtc aagcacgacg cggctaaatt cgcggatgaa
     1081 atccggatca tctttgcgtt cgggagccag ctgaatgaag gtcttaatgg aggtgagcgg
     1141 attgcggatt tcgtgcgcaa atcctccggc gatcgtttca agagatttca aacggtccgt
     1201 gcgcttcatc aagagatgtg accgctgaag gtcttccagc aacattcgcg tgtcgagggc
     1261 gttggtggcg gcttgggcga gggcattcag gagcgacacg gtggatggag agtcagagtc
     1321 ttgggagagg ctgcgaccaa aaagcgagaa cgcgatgaga cggccgcgac tgatatgcgg
     1381 taaggcccac tcgaatggca tcatccctgc ctcgttcgaa gcgtctcccc gtcgaattgg
     1441 gacacagcac gaccgggtca ctgtctgtgg agccgaaaac aggtgctggg ggatggtgtc
     1501 actgagcgcc aggataacgg gacagcagga ttcggccgtg acaccgacga tgcaggcacg
     1561 ccggtaatgc tcgtgttcgc gatccagcac atagagcgcg ccgtgacgcg tggaacttac
     1621 tcggcacagt tcccggataa ttcgttcacc cagtgcttca agatccgaca gggtgcctat
     1681 ttcatgggaa aaacgagtaa ccgaatccaa aagctcgatc gacgaaggcg tcgcgtctga
     1741 acccatctcg gttgggcgtg cgctcatagt gagagggagg cacaacgatg catcggcttt
     1801 gtcggcttag cgccgcctaa ggatctggag atgaattgga tgttcaaagt tgtacagcgg
     1861 tggagtatac accggcctag cgaatggagc tagaggaatt ctgtttcccg acgacggcga
     1921 ccagggcttg gccgtcaaaa atgggaagaa tgcgaaagga ctcgatgccg caatgccgaa
     1981 ggagggaagc cagcccggct tcgtcgaacg tatgaagaag ccggtggcgg atcgcttcgc
     2041 gtaagcgggc aaagtagtga aggagtggtt gagcttgggc gctgaactcg tcctggttgg
     2101 cctgcctgag atggcgtcga tagagcggcg agaggtcggt gtccgggtgg aacgtcgtga
     2161 cgatcagtga cccttctgga tgcagcgttc gatgccattc ctgcagcgcg accgccggag
     2221 atgggacata ggccagtgag agattacaga caagacgcgt caccgatccg gtcttaaagg
     2281 ggagggaact catccagtcg gcctggttcc atccgatagc cagcggcggc atcgctgaga
     2341 ggcggccggc aaatcccgag gcgagctcgc gctgtaggag cagcaccgca tggcgggcct
     2401 ggacgagtcg ctcgcgagcc cgtccgacac cgatcatgag tggcggccgc cggcctagag
     2461 gactcgcttg gcccgcccga taagtgtgat tcaccaatgc cgcacgcgtc aaatcgctgt
     2521 gccccacccc ggcatcaacg accacgctgc cggaatcgag cgggctcatc aaaccgtata
     2581 aatcgtccag taattttctg tactcatgca gattcccgag ttgaggcagt tgcgtcaggt
     2641 gggcggcgca gagcgcttca cgattgattt gcgaaccctc atgcctcagg aaggtgaact
     2701 cctcttcgat ccggcgctgt cgtgcgatga aggggtgggc cgaaatatcc tgttgggcgg
     2761 aaaggccctg aacggaaatg accgatccaa tgtgcatgag gagttgcttg aaggctgccg
     2821 gcagtgtgcc gtggacatcc aaccgccgct gggtcagcgg cgtggagagg tttacgagtt
     2881 tggtgtgtgc gcccaaggca gtcatgaacg cgtgcggaag atcggctgga ggaagggccg
     2941 ctgagacggc ggggcttgtg gcaatacaca agggcgatcg gatcaaccgg aggtcctcca
     3001 gagttgagtc aagatcggcg cagcgtgcgc cgatcaagtc gccgacaaac tggtcgacgt
     3061 tcacattgag tcccagcaca ttgcacacac cccgtcgcaa gccgaactga tagtccgcga
     3121 ggagatcatg cccatgcgca gtcatgagga gatgcctcac atcaacggca gggttgacga
     3181 gcagcagcag gtcgagcggg cgaatctgtg cggctgactt gagagcggcc cgtgccccaa
     3241 ggtcgctggc aatcacgagg accggagctt gaggccaggt atcccggacg aactcaatga
     3301 ctttgtccag atcatgttgc agactgcgca ggctggtgtg ttggagctcg ccatcgctgt
     3361 tgcccaggtg attggtgggg tcataccgga ggacgcggaa ttggtgtgcg gcgagataat
     3421 aggcgagtgc ggcatagtcc aatgcgactt gcccgtaccc gggggcgatg ataatgacgg
     3481 gccgactggc gtcggtcagc tgcaggtgat ggtcgtccat aatggaaatg gcctgacccc
     3541 gcgcatttct gcattcccgt gggatactga cgatcggcgc cgccgaagag ggttcgcttg
     3601 ctcgctgagc acgcaagtat tgccggatca cgcgattcaa ctcacgctcg gcgtaggggg
     3661 taagcgggtc aaagcggagt ccggcccggg cggcgaaatc cgttcccggt acgtgcaggg
     3721 cggttgaagc cgttcggtcc ggcgccgacc agatcacgtg agccgggagg atcgtgtccg
     3781 gagcaccggt ctcaagaccg tcgcctcggt gggcagaagc gaaatgaatc cgaagacttc
     3841 cctgtagatc ttccgggcgg gcttggatga gaagagcggc gcctgacctg ctgaggttcg
     3901 tcacttgggc cggaatgtgg ccggctggct ccgggcggcg actcgcttcc agccgggccg
     3961 gtaatcccag cgtaatccgg acggcttcgc gccggtcatc gtcaatttgg tccaacgtca
     4021 catccttgcc gggttcgagc gtgccggtcc tcctccttgg tgcgagacgt atatccagcg
     4081 tgaacagcag tgatcgttca tgcgtcgcgg taatcagcga ggcgagcact gccgcttcaa
     4141 tttgcggcgt tcgattcaag gccacaatca gatgagaagg agaaaccggc gacggctgtg
     4201 cgatggactc cggcaccacc cgttcataga tcaatccgtg caattccaag gtggccacgg
     4261 acgttgtcac cctgacctgc gcttcctgga gtgctatcga cggaaagtct tcgggcattt
     4321 gaatgcaggc tccacccaca ctcatattca cgactgttcc atcccactcg gatgacccgc
     4381 tgtgaacctt tgcctgcatg gcggtgggca ctcgatcgta actgcggcgg tcggtgcgcg
     4441 ccgccgtagc ccgcctgatg tcacgaggtg gcggaggcgg cgtcacggcg gggggatgag
     4501 cggccagggg cacgtctggt tccggaggcg cactcggttg tggcagagaa ggaaaagaga
     4561 tcgcagggaa gcgcacggtg atgcgaaacg gaccttgtgc cggtgccgag atgtcgagtg
     4621 cggcgccgag ttctccggct agttgaaaca gttcagcagc atcgagcgga ctgtgacgag
     4681 tgggccatgc ggctgccgcg accgtatgct cagtttcagt gatgtccacc tggacctgac
     4741 gggactgggt caagggaaac agggttggcg catccgcgaa tccgggcgga gtatccacgc
     4801 ttgttttgac ggccagtcca tgagaacgac cgactgccgc gataaagcca cccgcatacg
     4861 tcagtagccg tcggagaagg agagttgttt tttcgcggtc accctcgtag acgggaagag
     4921 ccgaagtcaa atcggacacc actacgccgg cagcagcgtc ggagaacgcc gacgtgagga
     4981 ggtcttcgag caagcgattg atcgtgtctg cgcgcgggac ttcttcggca gcgcgcgaca
     5041 attgttcgcc caattgtgcg gacgcggctg cgtgctcgat caacgcaccg aggccttcaa
     5101 taacgccgga tgcccgcagc cggtccgaaa gttgttgtgc ctcttgcaga agggtggaca
     5161 gcggatgttg gaggcgttgt gcgagatccc ccatttggcg gacgagcgcg cgaacatcgt
     5221 cagtccgctg ttgaggggca atttcgcgtg gtcgcggctg ccccttcttc tgctcaggga
     5281 cttgccggat gagacctacc gttcctacga atcgccggga gaggtcgtag accccccggg
     5341 cgctaacggc tgcggtcact tgcgacacac cggcagctgt cggctttccc agaagcgtca
     5401 actctacgcc agtggaggcc cggataccgg tacgtcgttc attgaagcga aatcgcgcga
     5461 gcggctgttg atcctgtgga aagagggtgt catacggacg accaatcagc tcatcgggat
     5521 gataacccag caaggcagac agactcctgc caagcgtaag gaagcggcca tcggcatcaa
     5581 gttcatacac aatgtcagtg agtgagtcga cgagccaaga atggcgaact tgcgcccggt
     5641 cggccgctac cctcaagtcc cgcttatcga atgcttcctt ggcacagaat agcaattcgg
     5701 tcaaaaagcc cggagactgc ttgttcagaa agaagtctgc gccattttca agcgcttcga
     5761 gggccgccgc ggcgtcggtt cgcgtgctct gcaatatgac agaggcgtga ggaatctggc
     5821 gtttgagttc atggacgagt gtcgaccgcc cgctcggaag gcagccttcg tcgatcagga
     5881 cgaccgccca tccactcgtc gttgcagagg ccatacggga ggcctcctca gtggaatagg
     5941 ctgcgtcaat gcggacgtcg tgaaaaaaac cacgaaggct aatggtaacg actttgatct
     6001 cttcggcgtg ctcgttgatg aggagaattg ttatgggctc gctctgcggc atcgaccgat
     6061 ttccaatgcg tagatcgtgt caaaggaaac acggttccag agcggagagg tcaccatcct
     6121 gcgcttgaaa aggccgacct tgtctcggcg cgggaagacg aatttctgct gctcggggtc
     6181 ggtatgtgat tagcacaggt gcgaggaaga aacccagcct ggctctggag gaaatggctc
     6241 ctactttcgt aggggaaggg aatcaagcgc actcaatcca cgaacaaaat tgcacaatac
     6301 tcggtggcat cggagggttc cccgcgggag cctcatccga tggttaatgg gcactggccg
     6361 ttttttgggt cggacaggtt cccggcgtca tgtagaggag ataattgccc atgccgtgct
     6421 gtggatcctg tttctgcagc gggtgatggt ggaccatgat catctcatag tcttcttcct
     6481 tgctaacgga aaagccttga gggtccttgt agacctgatg cggttggaat tccaggaatg
     6541 tcccgtcgag cgtgacatcc ggcacggtac gcaagagggt ctgctgtctg gttttattct
     6601 cgagggcgat gagtaacagt tcgtcgtgac catgcgggta cgcaaacttg acacagccat
     6661 ccatggaaaa tttcaagggg gcgctatgca cttggacgcc aggcttgatt tcaatcccct
     6721 catccgtttg gtcaggtccc cgctgggcaa atttactgta acacacgaca ttgaccccaa
     6781 cctgatagac ctccatttcc ttgacgggcc tgttctcggg cgccatatac atcgtgaaac
     6841 tcgccatgac gttcttggtg ggcggcgctt tgtgataaaa cgccacgacc gacatgaggc
     6901 gttgaccctt tcccagtttc acgccatatc cgtcagggaa tcttgcttcg gtcatctcta
     6961 atcccgctcc gctgaaaaac agcggctcgc ccgggcagga aacgctttgc ttgtcgttat
     7021 tgagcatcaa gatatgatga aggtagtttt tgggcagcgg cttgccgtct ttggtgaaga
     7081 ccgccgattt aaaccccacc atgtacatat ccttcggaag ctgaaacaca tgtttcggca
     7141 tggaagccgc caagtcgccc tcatggccgg aaggcaggtc gatcggtccg aatgtgagcg
     7201 tgaccgcgtt gtcggtgatg gccacaaccg tcgttgtctg atttttcaag gtcggaaccg
     7261 catggtgatc atggcccccg tctgccatca ccggcgcaga ccacagacac agcaccactg
     7321 ccagaagcac aaacatagct cctccaaaga ttaatgacag actcgtggct ggagtccgct
     7381 gccatcctcg cacccgccct ctatgatccg atcgccggct catcaagggg cggtggggac
     7441 cggagtgatc cgtttccact gataggtgcc accgtgttcg cggggcggga tatttttatg
     7501 ggtgccgacc ctggtatacc accagacgcc tttggcctcc ttgccgtctt ccgaaagcag
     7561 aatctcgaaa ccgccttcac ggtcattgcc cggctgctgc caggtgcctt gccagagacg
     7621 gtcagaaaac cggctggtga cgaaccgtcc cccgtgttgg gtatacggcc cgttgccggc
     7681 cttgtccagt gtcgcgcggt agcgtttgtc gtcttccact tcccatattt cccattcgcc
     7741 gcttaaatcc gtttgcgcga ggtgattgtt cgatgatgcc agggtgacgg aggcatgctc
     7801 gttcaaggca actgccccgc cggcagtgcg aaacgattcg tgccaggaca ggagcaccca
     7861 gtgtccgtct cgtcgctcca atacgcctgt ctcgcggagg ggaagcaggg cgcgctgcgg
     7921 ctggctgcct gtcgtgaccg tgcggatgta gtcgacttcc atcgcatacc aggctagatc
     7981 acctctcgtc caaactttga gttcccggat tgggatttcg atggttgccg ctgatttgaa
     8041 ttcctcctcc atgtcccgtt caaggtcgcc ccagccgacg tattttctcc ccccgatggt
     8101 gtaactggta atgtcggcgt catgagcgat cagtcgcgac atggtctcca tgtctttttc
     8161 ggcattcgcc cgcaccaggg ctcgcacagt agcttcgggg ctctgatcgt cggccgcgcg
     8221 tccgctggag actgatgcca acataacgat ggcactcagg acccacacac acggaccgcg
     8281 attcatgacg ttttggatct cccgagctcg tacgtacgga tggtgcgcag aagcagcttg
     8341 tggctaaatt gtgaggctac atggctggtg gagtcttgtc aatggccaga cttccgacct
     8401 tccccggcgt cgtataggcg cagacgatga cggtgacatt gtcttctccg cccgccctca
     8461 aggcttgttc gaccaactca tgacaggctc gtcggggatt tgcatgagtt gtcgctaaaa
     8521 ttgacccgat ctgggcatcg ctgagcattt tcgtcaaccc atcactgcac agcaacagcg
     8581 tgtcgtcagg cgccaaagga gtcgacgtga gatccggtgt catttcaagc cccatgccaa
     8641 gtccctttgc cagcacatgt ctgtccgggt acgctcgcgc ggtcgcttcg ttgatcaatc
     8701 cccgctgcag taagacttcc actaaggtat gatcccgcgt gatctgagtg aggacgccgg
     8761 catgaaatcg gtaggcgcgg ctgtcgccca aatgggctac gtgcgcaacg ggtgtcgggc
     8821 cgggaggaat ggtcatggcg acaacggtcg ttcccattcc tttcagagag gaattttcac
     8881 ggattttttc atggatggcc ttattggctg caataacggt ctcaatcaga ctctggtccg
     8941 gcggttggga cgattgttca cgagttgaga gcagagtctt ggcggtctgg gtggccacat
     9001 cgactgccac atgcgccgcg acatcccctg ccgggtgtcc gcccatgccg tcagcgatga
     9061 tccaaaagtt gcaactattg atcgcggcga agacgtcctg atttgtcttg cggacatggc
     9121 ccgtttccgt gcgtccgacg cccaaccagc gcggtgactg tttcataaaa tcattgaccc
     9181 gctggtttga cctgcgcgag gacagggccg atcgagggcg gaccgaagcg tgacaccatc
     9241 tgttcgtaga gcacttccgc caagggttcg aggtttctcg tatccgcggc gttgtagcga
     9301 agcagcaggt ccaatgctcc ttcatcgccg ccgcgccatt gataccacaa acgcacggcg
     9361 tcccatccgt ccaaaccttg aatctccggc tctcgatcaa tgtgcatttc acgctcgatg
     9421 tatttcagcc cccccgtgag tccgaggcgg cgagcggcaa agcacaaatc aaaatgcggt
     9481 ttctgcagat tgagccgcgg gaaagcctgt tgcaagaatg gaatgtcgaa tgcgcttccg
     9541 aaaaacgtca ccagcaaatc cgtgccttcg agtgcttccg ccaggcggtc ctcagtcaaa
     9601 ttctccccgc gaacaagact ggtcatgcgt ccgttccgat acagacccac caccgtgatg
     9661 acggcttggc cgctggactg gccggtcgtt tcaatgtcga gataggtggc gcgatgacgg
     9721 aacgtgtgaa acagacgcca atgatcccgg ctcttgagac aggttcgaaa aaagtccgca
     9781 ctgcccccgg caagccgcga ttgggccgtc gcgaggactt cgtcatacca gccttttctc
     9841 gtcgctgaga tgccggtaat gagagcgcga ccgagaaacg cggaccaatc tgcaacgcca
     9901 tcctgccaga ggcggcgttc cgttgccgga ccgattccct tgagcagtac gaaggtcgag
     9961 gtcagcatgg tggaggggat tgtagccttg atttcaccag ttaaacaagc tagagtccgc
    10021 gatgggagcg ggttttcaat cgccgcgctt cacgcaagga gagcactgtt cgttggctga
    10081 tcccgggaac aaaatccgca tcattgtcgg gggggtgcag gttgaggcgg agctgaaagg
    10141 cacgaagacg gctcgcgagg tctatgcggc gttgccggtg gaggcgcccg tgaacacctg
    10201 gggtgaagag ttttatttta agctggacgg cgtccgggat tatcgggaaa cggcaacgaa
    10261 tcaggtcaag gtgggcgatg tcgcgttttg gggagcgggc caagtgttgg cgattttctt
    10321 cggccggacg ccgatgagta tgggcccgga cccagtgccc gccgatcgtg tcaacgtgat
    10381 cggacgcatc cttggagatc ccacggtgtt gcgaagtgtg atggcggcgc cgtcgattcg
    10441 cgtggagcgg gtgtaacgca tgcggctttc caaagagcgg gtccgccata tggccgaaac
    10501 tctggccggg cggttggtcc aagaaagtca tgtggccctc accggtacac aaaaggcgtt
    10561 catcgagtcg cttgaccagg tggtgaccga tgagctgtcg atcgaagacc ggttgaatag
    10621 cgaagtccgg cagctgctca aggcctacga gcgtcaaatc gaaaccgggc aagtggacta
    10681 tcagaagatg tttaccatga tcaagcagaa gctggtacgg gagcggggta ttatcctgta
    10741 aacgcgatcg ggcgctcgcg gatcgaggca atagacggcc ggtcaggacg gactgaccct
    10801 atgttgagcg aggacaaagt cagccatttg tcgcacgtca tcctgcttgc ggtaaaaaac
    10861 agcccgcacg ttgaggtgaa aagcgaggac gcacggatat tgaaggatat taaacgcgtc
    10921 cttgccaccg aattggccca ggaacaggag atcgaccgaa aagtgagggc gaagctgggc
    10981 tcgtacaccc gcggcatcgt ggaaggcagt cctgagtggg acgtgcttta tcgcaagacg
    11041 ttcgaggaag agtcgcggaa acacacgaaa tgagaaggcg tatgagtagg agggtgtcat
    11101 gaaattcgga ttcccggtcc tcgttgcgct ggtgctgatc gtcagcctcg gagcctgcac
    11161 aaagggaaaa gccaggccgc tcgttccctt ggccctcgaa ccccatgcca aagcgccggc
    11221 tgtggccttg actgagcagg gaatgcaggc ctatcagacg cggaaattcg atgaggccaa
    11281 acggtatttt tcccaggcgg tgacggaggc gccgcaatca ggaccggcac attacaatta
    11341 cgcgctggcg ctgaacgcgt tgggcgatac cgaagaagcg aggcaacatt ttattgaagc
    11401 ggccaatctg gcgccgggcg acaaaaccat ttgggattcg ccggcgctcc atcagtacgg
    11461 gaatccgcaa actgaaaagg aaaccaagga acatcggacg accactagcc gcccgacgtt
    11521 cggcggccct cctcggtaga gaggactgag aatatgccca aggacatcgc ggtgggagac
    11581 aaagcgccag actggtcatt gcctgaccac acgggcaagc cggtgacatt gaagagtctg
    11641 aagggcaaac agatcgtgct ctatttctat ccaaaagacg atacacccgg ctgcaccaag
    11701 gaatcgtgtg attttcgaga cgtggaatca caaattatcc gggcgggcgg cgtcattctc
    11761 ggtgtcagtc tcgacgacaa ggagtcgcac caaaaattcg tgaaaaaatt tggcttgccg
    11821 tttcccttgc tcagcgatga agatgccgcc gtctcgaagg cctacggcgt ctataaagaa
    11881 aaaaatatgt acgggaaaaa gtactggggc atcgagcgta gcactttcgt catcgaccct
    11941 gacggcaagc tcaaggccgt tttccggaag gtcaaagtcg acgggcatgt cgacgaagtt
    12001 ctcacggcgt tacggtcgta gtctctccca ctatccttcg cagtcccatg gcttgatgcc
    12061 ggtcaatgcc cttctccctt tcagtccgca caaccgcgct tgtggttttg gtccttttcc
    12121 tgaccgttgc cgcgccgata ccagcggtgg agaagatcac cccgacagtg cttgtgaaag
    12181 atagcctgag cgcaccccac caacagacca ccatcgaagc caaattgttc gccaaagggt
    12241 tgttgaaaga ctcgccgctt ggcggagaac cggttgaact cctcgtcaag ggaatagtgt
    12301 cggcaacagc gatgaccgga ggagacggaa gggcattcct ctcgttcatc ccaaaaggca
    12361 aggacatcct gcctatccag gtgcgaaccg gcagcagccc gcgcgtgtcg tcaggggaag
    12421 gggaagcgca tctggttgtt tgggaacgtc gcacgccgat tctgatagta gaactgaccg
    12481 cgctgatgga ggacgttcat gcggagacgc cggtttctcg gttggtcccg cgcatcgacc
    12541 cggaagcaag gccgatcgcc gatgccgccg atgaactcgg gaagctcact cagttctact
    12601 acggtgtcat ctatgtcgtg acggttcctg caggggccga tgtgtttgtc accagcgtgg
    12661 aagccaggac ctggctctcc acccacaagt tcccccgggg gttcgtgctg gcactctcag
    12721 ggggagaaga cgcgctcgga gcaaaaatcg atgagcttca tcaggctggc tggaagtcgg
    12781 ccaaaacggg gatcggtcgt tcgaaagcct ttgccgagac gttcctcaag cgtcggctcg
    12841 aggccattat cgtacccgag ccgcccgtcg gggaggtgcc acgcaaaggc aaggttgcca
    12901 aagactggaa ggacgtgcga aaaaaattgt agcagccgac cggtgccctt cttgctcgcg
    12961 cagtcgacta tccatctcct tcctttggtg tcagctttgc tctatgataa agcctcgttc
    13021 cactccatga ggctttccga ttcatgaaat ccaaatcgat cttttcctgc caggcatgcg
    13081 ggcatcaatc gccacgttgg ctcggacgct gtccggattg cggcggatgg aacacgatca
    13141 aggaagaacg gcatgccccg acggggaagg ggcgccccgt tgccatgaac ctggcgcagc
    13201 caacggcgac cccgattgca aagattgaag tgatcggtga agaccgccgg ctgactcgca
    13261 tcagcgaatt cgatcgagtc ctgggcggcg gagtgattcc cggcgccgtc attttgatag
    13321 gaggagatcc gggaatcggc aagaccaccc tgctattgca ggctctgcct cgcctggcgt
    13381 cgaacgtgca accggtgctc tacgtctcgg gggaggagtc gcctcggcag atcaagatgc
    13441 gcggccagcg gcttggagtc gatcatccga acctcctggt cctcgccgaa acgtcgctcg
    13501 aacagatctt gaaggctgtt caggacatac aacctgctgc cgttgtggtc gattccattc
    13561 agacggtcta cacagagcaa ttgacttcgg ctcccggcag catcagtcag gttcaggaag
    13621 tgtccggtca gctcatgtgg ttcgccaagc gatcgggcgt gcccgtattc atcattgggc
    13681 atgtcacgaa agaaggagcg attgccggtc cgcgcttgct ggaacacatc gtcgataccg
    13741 tgctgtattt cgaaggagac aagggacaca gctatcgcat cctccgtgcc gtgaaaaatc
    13801 ggttcggctc taccaatgag atcggtgtgt ttgagatgaa agagtccggg cttgaagaag
    13861 tcacgaatcc ctcacaactg tttttgggag aacgcccgca gcgaagcaca ggctctgtgg
    13921 tggtgtcgag cgtcgaggga tcgcgtccga ttctcgtcga gttacaggcg ttggtgtctt
    13981 cgaccagtta tgccatgcct aaacggatga caaacggcgt ggatctaaat cgcgtctcac
    14041 tcctcttggc ggtcatggaa aagcgcttgg gcatgcattt gtccggtcaa gatgtgtatg
    14101 tgaatgtggt cggaggcatg catatcgatg agcctgcgat cgatctcggc atcgttgcgg
    14161 cagtgacctc gagtttgagg gaagtgccgg tcgagcccgg attgctggta ttgggcgagg
    14221 ttggtttggg gggcgaggtg cgcggcatta gtcaggctga attacggata cgtgaagcag
    14281 cgaagatggg tttcaagcgg tgtttgttgc cggagcggaa ccttgcaaag ctcgagccgg
    14341 ttgacgggat ggaactcgtg ggaattcatg aagtcggaga agcgctggat gtcgtattgg
    14401 cgtaagagcg ccctgtgcct gctgccttac ctgacggtgc tgtgcttcgt ggccgccggc
    14461 tgcacgactc ccgtgcagcg ggaggaagcc gacacgaagg cggccaccgc agacgagttg
    14521 acagctcttt tggagcaacg ggagcgcgcg gttcagacga tgaaaggctt gttcagtgcc
    14581 aaggtccgcg gaggcatcat ccctattacc acacgcgtgg aaggggcggt gtattaccga
    14641 cgccccagcg cgatgcggtt acgcggcttt accgccatcg gcagtgaact gttcgaattt
    14701 ctccagacgg atgatcagtt cacattacga ttgccgacga tgggccgggt cgtgtccggt
    14761 catccttccg acatgagcga gatgggcaag cttgcgaggc catttcaact gagcgtctgg
    14821 gcaatgagcg gagtccttgg aaccggcacc atcaacgcgg gcgacacagt cagggttgtg
    14881 gacgaaggag atcgtcatcg gctcgaagta ttccgggcct cgccttctgg aggtctcacc
    14941 attcatcggc gactctggtt caatcgagag acacgcctgg tggtcaaaga ggatcgattg
    15001 acggagctcg gtgagatcga tgccacgatt gtatacgaag attttcgtcc ggttggcgag
    15061 atgaccggtc tttccatttc aggcgatggc cagttactcc ggccgttcaa gatctccttg
    15121 gaagatgggc aggggcgtgg cagcgtccaa gtgaccttcc acgagttaat tccgaatgca
    15181 acactgaagc cgagtgaact cgctcacgcc tcaaacgctc ttccagatac cggccgactc
    15241 gcgaccggaa cagatggcgc acgccagctt ccgaatcaag acctgcccta gagtcgttgc
    15301 cgctcgatga cttaggcgat aacgagaacg cggtaaagca atgattcgat gaaagtcctc
    15361 gcggtcgaaa cagcgacgtc atggcagagc gtggcgatcc tcgatgacga tcgcgtgctg
    15421 gcacgctgcg atcaggatgc cgccggtgcc catgcgagac tcttgttacc ggcggtggac
    15481 aaacttttct cacaaaccgg gctgggaccc gccaagctgg acggactcgt cgtctccata
    15541 ggcccaggct cgtttacggg cctccgggtc ggcctttcca cggtgctggg atttcggacg
    15601 atcacacggg tgcctttggc tgttgtgcca acactggaag ggatggcctg gagtttgcgg
    15661 gcctcgaacg aacgtctctg ccctgtgttg aatagcagac gaggcgagct ctactgggcc
    15721 atctttcaat ggcaaggccg cgaccggctg gaccgtctca cgccagaaca ggtcggatca
    15781 gttgagacgt tggcgaagca actgagcgga tccgtcatga tgttcggtga agggtgggag
    15841 acggaaaggg cggccattca ggcgatgggg cgttcggtga agatcatgga agctgaccag
    15901 cttctgcggc cctcggctgt ctccatcggg atggccggca ttgctcggct caggaggggt
    15961 gagcaggccg gtgtgggcat cagcccgttg tacgtgcagc gcccggaggc tgagattaag
    16021 tatgaggagt ccggcgggat ctcaccgatg tcaagacggc aacaaaaact cgcacggaaa
    16081 cttcgcaacc gcccgcctct ctcggcaacc aagaccaggc gccagacatg atgccgcctg
    16141 actggacgat aggggccgca aaccttgaat ctctttcaga aattttagca gtagaagaag
    16201 cttgcttctc ggcaccctgg acgcgcaaaa tgctggaagg agagttgagc ggaaaccctt
    16261 tcgcccactt tctggtcgca aggccgaggc gcgtggagga agcgtcgaaa caggcgatcc
    16321 tcggctatct gtgttattgg atcgtcttcg aggaagtccg gctgatgaac ctggcggt
//
LOCUS       DASSCQ010000156        28563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1807498, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000156 DASSCQ010000000
VERSION     DASSCQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 28563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28563
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807498"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..529)
                     /locus_tag="VFH05_10835"
     CDS             complement(125..529)
                     /locus_tag="VFH05_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HET6678758.1"
                     /translation="MAVLRDELIALRSTDDIIKARQLVRDCAVLQGLSLVDQTKLVTA
                     ASELARNTLVHGGGGEMCLQAVHEGTRRGVQVTFTDQGPGIADVELAMTDGYTTRGGL
                     GLGLSGSKRLVNEFAIESKPGKGTTVRIVRWK"
     gene            complement(510..893)
                     /locus_tag="VFH05_10840"
     CDS             complement(510..893)
                     /locus_tag="VFH05_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6678759.1"
                     /translation="MERIPILKMGPYLLVTIQVDMHDRVAMTLQDELTERIVAERARG
                     VLLDISSVDVVDSFIGRMIGNIASMSRVLDAETVVVGMRPAVAITLVELGLALPSIKT
                     ALNVDAGMALLKSAGKMSGNGRLTR"
     gene            complement(946..1809)
                     /locus_tag="VFH05_10845"
     CDS             complement(946..1809)
                     /locus_tag="VFH05_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6678760.1"
                     /translation="MSQNSQMADEIRNRQDHILKHWMDYQLSALTLRRDRVKESDLRE
                     MSRRFLDLFSEAFSASADASSPSWKPVKEHLAEVTRSRAEQGFSPSETATFVFSLKQP
                     LFDLAQQRAGQDSKKLAEMLWDLTALLDKLGLYTTEAYQETRESVILRQQQELMELST
                     PVVRLWDEVLALPLIGTLDSARTQVVMENLLQQVVQTGARIAIIDITGVPTVDTLVAQ
                     HLMKTVAATRLMGADCIISGIRPQIAQTIVHLGVNLNDIVTKATLADAFLIALQRLGA
                     TVTRNTASTSG"
     gene            complement(1914..3686)
                     /locus_tag="VFH05_10850"
     CDS             complement(1914..3686)
                     /locus_tag="VFH05_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678761.1"
                     /translation="MATLRVLIVEDDATDAELVVRELKKSGFESESRRVDTRDAFMIA
                     LEESTPDVIIYDHNLPHFSSSEALELTRQRDQAIPFIIVSHAIGDEDAVALMRAGAAD
                     YLLKDRLGRLGEAVRHALEQRRLREEHTAARNAIVSLNADLELRIASRTAQLKVANHA
                     LEMELGQRKRAEEALRRLNGDLEQRVTERTRDLVASNQRLQALATELTLAEQRERKRL
                     ASELHDYLAQMLALGRIKVTQLRQKLSGQAPAIQQLVGEVDNLLEKAGEYTRSLMAKL
                     NPPVLDELGLPSALTWLAKQMPLHGLLVEVRLLNEQVSLPDDQSVLLFQSVRELLINV
                     AKHAGTDRATVSLKVDQEQQLLIEVQDSGRGFDPVAMEAKSENGHFGLFSVRERMQAM
                     GGRLEVKSIPGQGTTVTMMLPLAKAKTAGKPSDVAAMATEPRQESEQSIYRIPHAPFH
                     PPPGNTIRLLLVDDHTMVRQGLRSLLESYEDVVIVGEGSNGEEAIELVEQLRPALVVM
                     DINMPKKNGIEATACIKARFPETVVIGLSVQAGEEARRAILNAGGAVLLTKEAAVDEL
                     YRTIREVLDAQPADGSREGVAEVR"
     gene            complement(3707..4177)
                     /locus_tag="VFH05_10855"
     CDS             complement(3707..4177)
                     /locus_tag="VFH05_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678762.1"
                     /translation="MTNGTEILLIEDNPEDVEITLRAFHKHHIANRVHVVRDGEEALE
                     CLFGTGRYAERSPCRNTKLILLDLKLPKVDGLEVLQKCKSDPRTKGIPIVVLTSSREE
                     QDLVKSYNLGVNSYVVKPVDFPQFSDAVRQVGLYWILLNQFPSELALESASQSN"
     gene            complement(4174..7302)
                     /locus_tag="VFH05_10860"
     CDS             complement(4174..7302)
                     /locus_tag="VFH05_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6678763.1"
                     /translation="MTHREASGVTFEAGIGGHLKQSGLTAVIKEHLVALFATSLALGC
                     RLVLDPYLGDHLPYVTFFVSIAFTTWYAGVSAALTATLLGGVAAVWFFVPPRFSFDIT
                     GLSQQVGLVAYGGVGLTFIAVGQVMHRARRRAEELAQGLRVTEERLTLAQQASQVGSF
                     DWNLDTGVNSWSPELYAMYGLHPDKFDKTQAAWESCLHPDDRHEMVEAVERSRRSGEP
                     EDREFRIVRPDGEIRWLAGRWRWLRDQTGQPVRLTGVNFDITERKQDQEALRLSQSRY
                     RALIQSLPAAVYTCDAQGRIQLYNQAAVSLWGREPEIGKDLWCGSWKIYRPDGTPLPL
                     DEFPMAVTLREGRAVRGEEIVIERPDGSKRHVMPHPDPIRDASGTVIGAVNMLIDVTE
                     HKRIDDHLRESEDRTRAIFESSLDALITMDADGLVQDFNPAAEKTFGYTQAQALGRPV
                     ADLIIPPRLRESHKVGLRRYLETGEASVIGRRIQMPALRADGSEIPVEFSIAVTRRPG
                     CPPFFTAYLRDISDRQRSERATAHLAAIVASSDDAIVSKNLQGMVTSWNRAAERLFGY
                     RAEEMIGRPVTLIIPADRQDEERRILDAISHGRTVEPYETIRRRKDGSDVHVALTFSP
                     LFDTLGKVIGASKIARDITPRVEQERALKESRERLRHALAYQEAVINNMGEGLYTVNR
                     HGLVVSMNQAAEKLFGWNKEELLGLKMHDVTHYKHRDGTPFPAQECAELQVLQKGTSL
                     INHQDVFVRKDGTCFDVVYSAMPIRAGEEIAGLVVVFRDVTEEKRAEQALRDRDRALT
                     IANDELTQQKSGLADANKELQSFSYSVSHDLRAPLRTIDAYIRILEEDHGAHLNSEAR
                     RCLGIVRKAAGQAGELIDDLLEFSRLGRIGMDFRPTKMAEIAREVADELNVTHDGRTV
                     DLTIGDLPPCQGDWRLLRLVWMNLLANAFKFTRHRPVAQIEVGWLPDDRQADASVYFV
                     RDNGVGFDMKYVHKLFGVFQRLHLKEEFEGTGVGLAIVQRIVQRHGGRVWAEGKVDGG
                     ATFFFSLRKALR"
     gene            complement(7443..7754)
                     /locus_tag="VFH05_10865"
     CDS             complement(7443..7754)
                     /locus_tag="VFH05_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678764.1"
                     /translation="MNAIALFLMLVLNGNMTCGAEPPDQAMLGDQAVQVQVFDCYWSE
                     RESASHQFVVISPVCPTYVAQPILLKEVHAHKGWVMNRFGEFYPATTNIEMMDVYRPP
                     C"
     gene            8143..9258
                     /locus_tag="VFH05_10870"
     CDS             8143..9258
                     /locus_tag="VFH05_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6678765.1"
                     /translation="MTRKALPNEPSSITRRQSRSHAIEFGSRRPDLRSVSLTGLFILA
                     LVWFLHQAQDFAVPLTLALLLHFLFMPIVRWFKRHHVVEPVAAAIILLAMTGLVTMTM
                     YELAAPAAQWLEKAPRAFRVIESKLLPVKESVSQLSEATEAMNRVGQTDSSPAVRKVE
                     VVPDRRAAVVSLLGKTMAGIGTTLLLLYFLLASGDLFAKKLLTVLSRREDRGCAADIL
                     EGLERQMFQYLFQTVVIQSGFGFVLWMSLWALGMPNPGLWAAAAAILQIVPYIGGLAV
                     LVLIAVVAAVTFDNTWSIAGPVIAYLSLTTLKGFVAPVLMGQRLVLNPVAVLVSLVFF
                     GWMWGLAGTLLAVPLLACFKIMCDHIESLERTGEFLA"
     gene            complement(9518..10264)
                     /locus_tag="VFH05_10875"
     CDS             complement(9518..10264)
                     /locus_tag="VFH05_10875"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET6678766.1"
                     /translation="MKQLRRIRELTDLTLLATDGEIGNVQELYFDDRTWAVRYLVVKA
                     GGWLLSREVLLAPAAVADINDANGTMSIALTKEQIKAAPSIEAAKPLSREYEEAYFRH
                     FQWAPYWEPGPTALGSSLPFPETPPIPIDTTLPADAPKNPHLRSTSEVIGYDIQAKNG
                     TIGHVEDLVVDDEAWTVRYVEVDTKNWLPGKKVLLQTMRIDHISWAERSVAVMLSQQA
                     IESAPGYDPSRLITPAYEVQLFKHYGSEAA"
     gene            complement(10261..10581)
                     /locus_tag="VFH05_10880"
     CDS             complement(10261..10581)
                     /locus_tag="VFH05_10880"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HET6678767.1"
                     /translation="MTPLAHILLGAVIMGDVLAALFFVRFWKMTGDRFFLFFSASFLA
                     IAVSRVVVDESIPPFGMEVFGYMIRILSYLFIIAGILYKNRGTKTSRNLAPPSTVFPQ
                     EHLS"
     gene            complement(10578..10847)
                     /locus_tag="VFH05_10885"
     CDS             complement(10578..10847)
                     /locus_tag="VFH05_10885"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HET6678768.1"
                     /translation="MIIAPIVIYSLCMLTALTCAWLLLQAYIKTRYRLLFWCSVFFCI
                     AAMNNVFLVLDKVVFPDVDLMVYRYAVALTGLMILLPALILEKEV"
     gene            complement(10844..11269)
                     /locus_tag="VFH05_10890"
     CDS             complement(10844..11269)
                     /locus_tag="VFH05_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678769.1"
                     /translation="MEGETSRKHPMDAVVPSEVQRFVLDRIDSIAQLEAILMMRNAPD
                     TWWPSSTLAERLYIADQTCRSELDGLCQNGLVVAREEDIGWSYRYAPSNGELREFVDR
                     LVYYYSHHLVPISNLIHGKPRTRIHEFADAFALKKKDGK"
     gene            complement(11272..13698)
                     /locus_tag="VFH05_10895"
     CDS             complement(11272..13698)
                     /locus_tag="VFH05_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678770.1"
                     /translation="MPKANILIVEDEAVVAADLAGKLERAGYRTIAVASDGEDAIETA
                     KALGPDLVLMDIRLAGSLDGIKAAERIQAFRSIPIVYLTAHSDMATLRRAAATEPFGY
                     ILKPFDERDVTTQIEIALYKHQAERRLRESEERYRKLVETAMDSIITFDHAGVIHSCN
                     PATEQMFGYRVEEMVGRSISMLIPAWFAQGAEESSADAANRLTRLIGLWRELNGQRRQ
                     GDAFPVEIAVSESFSGGEIRYTGILRDISERKRTEAELRWRAGLLAQTHDAVVVWRMG
                     GGVIYWNHGAEELYGWKVAEAAGQPIHQLLSTALPLSEEEFHRQLIEDGRWEGEIRQL
                     TKGGQSVVVESRMVTLTEEYGGILVLETNRDVTERRAMHEKVCRLAEGLEDRVKERTK
                     ELVQSQSLLRQLTSELTIAEQRERRRIATDLHDYLAQLLVCARLKVSQSRGRMAEAEV
                     EGWLGESDDILQQALTYTRSLVAQLTPMALHEFGLVAALKWLGDQMRQQYRLSVDVDV
                     QAGLSVKLPEDQAVLIFQSIRELLINVAKHARVDQATIRIQPQDGQLSIAVVDEGLGA
                     DTLTSSSGARSSKFGLFSIGERMRALGGAFDFTSSPGKGTIATLTMPLPPADAPAEAP
                     PVTTGETMTDRRRPSDEEYAQQLTAQRSAFRSAPELVSSASHLEERLRIRVLLVDDHA
                     LVRNGLSSVLRYNTDLQVVGEAADGQEAVALAAALKPDVVVMDVNMPKMDGVEASRRI
                     KQHRPSTVVIGLSMHEGGHHEAAMRESGAAAYLTKDAAAERLIETILRCRAASLTGSS
                     ASFSSSALTE"
     gene            complement(13670..14827)
                     /locus_tag="VFH05_10900"
     CDS             complement(13670..14827)
                     /locus_tag="VFH05_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET6678771.1"
                     /translation="MTNAMTTFKEPANILLVDDDSRNLDVLDSILACPDYRLFRARTA
                     DEALLALIAEEFAVIVLDVRMPEVSGYELAQLIKQRKRTQHIPILFLTAYYREDDDVL
                     QGYGAGAVDYLSKPVNPLILKSKVAVFVDLFRKTRALATMNRAMEVEIEERLKQLKAS
                     LHEKDILLKEVHHRVKNNLQVISSLIDLQAERLTDPAMRTLFRDTRDRVRSMALVHEK
                     LYQSADLAHTELGAYIRNVMKELFLAHGEVSSKVTLQLQLEPVFLPVDMAIPCGLILN
                     ELATNSLKHAFVGRERGTIEIQLTSVRNESVRLRFRDDGWGLPAGLNLDAAESLGLRL
                     IRMLSKQLRGEVKTHSGEGTTFELHFQLQNTRGGSSDEKAVLDAQGEHTHR"
     gene            complement(14935..21240)
                     /locus_tag="VFH05_10905"
     CDS             complement(14935..21240)
                     /locus_tag="VFH05_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET6678772.1"
                     /translation="MKHQNDEQETTALEDAPVAANGGTRRRHDINEQLGEILKVMVAY
                     RNGDFSVRLPATWTGVLGKMSDTMNDILSVSQRRAEEVVRISRVVGKEGRLRQRLSVP
                     GVTGGWSDEIESLNTLIDDLASPTTEVTRAVGAVGKGDLSQAMALEKDGRPLEGEFLR
                     SAKLVNKMIEQLAVFTSEVTRVAREVGTEGKLGGQAQVKGASGVWKELTDSVNQMAGN
                     LTAQVRNIAEVTIAVANGDLSKKITVDVRGEILQLKEAINTMVEQLRSFASEVTRVAR
                     EVGTEGKLGGQAVVPGVAGTWKDLTDNVNSMASNLTAQVRNIVEVTVAVARGDLSRKI
                     TVDVRGEILQLKETINTMVEQLRSFASEVTRVAREVGTEGKLGVNADVPGVGGTWKDL
                     TDSVNAMANNLTAQVRNIAEVTTAVARGDLSRKITVDVKGEILALKNTINTMVDQLNG
                     FAAEVTRVARDVGTEGKLGVQAVVPGVGGTWKDLTDSVNAMGVNLTAQVRNIAEVATA
                     VAKGDLSRKITVDVKGEILELKNTLNTMVDQLNGFAAEVTRVAREVGTEGKLGVQAVV
                     PGVGGTWKDLTDNVNSMANNLTAQVRNIAEVATSVARGDLSRKITVDVSGEILQLKET
                     INVMVEQLRSFASEVTRVAREVGTEGKLGGQAQVKGVSGVWKELTESVNEMAGNLTAQ
                     VRNIAEVTVAVANGDLSKKIIVDVRGEILQLKEAANSMVEQLRSFASEVTRVAREVGT
                     EGKLGGQAQVKGVSGVWKELTDSVNQMAGNLTDQVRNIAEVTIAVANGDISKKITVDV
                     RGEILQLKDAANTMVEQLRAFSSEVTRVAREVGTEGKLGGQALVPGVAGTWKDLTDNV
                     NSMASNLTAQVRNIAEVTIAVANGDLSRKITVDVRGEILQLKEAINTMVEQLRSFASE
                     VTRVAREVGTEGRLGVQAVVPGVAGTWKDLTDSVNAMGANLTAQVRNIAEVTTTVARG
                     DLSRKITVDVKGEILELKNTINTMVDQLNAFAAEVTRVAREVGTEGKLGGQAVVQGVA
                     GTWKDLTDSVNVMATNLTDQVRGIVKVVTSVANGNLRQRLTVEAKGEVAALGETINNM
                     TDTLATFADQVTNVAREVGVEGRLGGQANVPGAAGTWKDLTGNVNLLAANLTTQVRAI
                     AEVATAVTKGDLTRSIQVETRGEVAELKDNINTMIDNLRVTTERNQEQDWLKTNVAKF
                     TRMLQGQRDLFTVGQMLLSELAPLVEAQEGTIYQMDIVEGQKPSLRLLASYASHREPP
                     ERVGVGEGLIGQCAVEKQRILLTDVPPSYTVIHSSLGEALPANIVVLPVLFEGQTKAV
                     IELASLHTFRVSHITFLEQLTQSIGVVLNTIEATMRTENLLQQSQQLTSELQSQQKEL
                     TQTNEELERKAKQLAERNTEVERKNKEVEQARRALEEKAAELALTSKYKSEFLANMSH
                     ELRTPLNSILILGQQLGDNTTGNLTQKQVEFAKNIHSAGSDLLNLINDILDLSKIESG
                     TVTVEAEEMTFSALRESVERNFKHIAEQKNLPFHLEFESGLPRAFTTDPKRLQQILKN
                     LLSNAFKFTSHGHVSMNVRIATEGWTTEHPVLSRSHTVMALAISDTGIGIAPEKQKLI
                     FEAFQQADAGTSRKYGGTGLGLAISRELATLLGGEIKLTSEPKQGSTFTLFLPLVYTG
                     PSSAGVRPSAAAVALPGQAPALAILPVVGEEKIADDRDTVQDGELVVLIVEDDPHYAR
                     VLLGLARDKGFKGIVATRGKQALDLALEYVPAAITLDIFLPDMLGWTVLNNLKLNPKT
                     RHIPVQIISLEEERQHGLAHGAFSYMVKPVTTEGLESCFDRIRNFITPHLKRLLVVED
                     NELESRSITELLLHDDIEIVNVGTGAGALEALRSQSFDCCVLDLRLPDMSGFELLDQV
                     KADPTLGNVPIVVFTGKDLSTEEERRLKAVAKSVVLKDVQSPERLFDETALFLHRVVA
                     DLPEPKQQLLERLHQSNEALHGHKVLVVDDDARNIFALTTVLENQEMDVMSATNGRHA
                     IELIENTPDLSVVLMDIMMPEMDGYTTIREIRKKPEFRSLPILALTAKAMKGDREKCL
                     EAGASDYVAKPVNTEQLLSMLRVWLYR"
     gene            complement(21699..22691)
                     /locus_tag="VFH05_10910"
     CDS             complement(21699..22691)
                     /locus_tag="VFH05_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678773.1"
                     /translation="MRSIPLCLVAVSSLLLTACASTLPPGKATSFDRRCLPASMDLRR
                     MPLSEMDKPAATSLSAERQEAVKLYSTIAVHVADVMDLLPLLNHLARLENQHASSAEI
                     ERARRKLTTRLQLANMEVSSLVAEIECEVQRADEVQDRLKQVQTTRTTTQTILGIIAG
                     GLANILSGGIGMATRAGDAADIASVAGGTLEVLFGTSANFTKVRQEFTHPHNHLQAFW
                     NGEGKEKEFFPPGIWRFLTEPDIRDLEGHSLRDVLVQTWNEEGRLGAPGSNQEKERKA
                     LLFGEGGLYDSEDLHVREAMLHQLESSIQLMHQDLETLLREVLLRQALEEDGVS"
     gene            complement(22710..23843)
                     /locus_tag="VFH05_10915"
     CDS             complement(22710..23843)
                     /locus_tag="VFH05_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678774.1"
                     /translation="MSERERTIGRKSVQSPHVGGHAVADGKSVARSAGLHYVDDRRPG
                     IARVRQRHGMRYLWPDGRPVRDGETLTRIRSLAVPPAWRDVWICLDADGHVQATGRDA
                     RGRKQYRYHPRWREVRDGNKYGRMIAFGRALPLIRKRVEKDLARKGLPREKILATVVR
                     LLEVTLIRVGNEEYAKKNDSFGLATMRDRHVRVNGSALRFQFHGKSGILHAVDLQDRR
                     LASIVRQSRDLPGFELFQYLDETGQRRTIDAADVNAYLKTIAGDEFTAKDFRTWAGTV
                     LAAYALREFSAVDSQAQAKRNVLRAIETVAKRLGNTKAVCRKCYVHPDIVNAYFDGTL
                     AASLNRRTGRELRSHVRDLPPEEAAVLALLHRRLGQDACRAAA"
     gene            complement(23958..24824)
                     /locus_tag="VFH05_10920"
     CDS             complement(23958..24824)
                     /locus_tag="VFH05_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET6678775.1"
                     /translation="MPRVVWKGAISFGLVHIPVGLYSAEKRNSFDLTMLDRRNMKPVG
                     FKRYNKENGKEVTWDDIVKGYEYEKDRYVVLTDEDFRRANVEATQTVDILTFVEQEQI
                     EPIYFETPYYLAPDKRGHKGYALLRETLKQTGKTGIANVVIRTRQYVAALIPVGDVIV
                     MNTLRYSNELRDTAELDLPSSNLKSVGVNPREIEMARRLVDGMSAAWKPQEYKDTYHE
                     DLLALIEKRIKAGQTEVVTEPSPEEKKRPAKGEVIDLMALLKKSVESKGKAHRPAAPA
                     KARSRRSGRRAA"
     gene            complement(24900..25832)
                     /locus_tag="VFH05_10925"
     CDS             complement(24900..25832)
                     /locus_tag="VFH05_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6678776.1"
                     /translation="MSRTAGTSPAIRRTVSHPWTLAGMTLAELGRRLWRKSQEHELLG
                     RAAQLSYYALLALFPALIFLTALMGLFSVQSFMPELMSYLHNVLPEDALSMVERFLTQ
                     VAEGSGANILSLGALGALWASSSGVTAIMDALNVVFGVKEDRRPFWRVRLTAMVLTIG
                     LAGFVILSLALVLYGPTIGRWIANLFGLGVAFTWLWNVLQWPIIVALMLVVVAAVYHI
                     CPDTREKRWHWLTPGSVFAVSMWLVVSLGFKAYVDNFGDYNKVYGSIAGVIVLMLWFY
                     WSGMVLLLGGEINAEIAQASREGAGHKGEVRQAA"
     gene            complement(25832..27406)
                     /locus_tag="VFH05_10930"
     CDS             complement(25832..27406)
                     /locus_tag="VFH05_10930"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET6678777.1"
                     /translation="MSKHRRAWTIALIAVAIVILAVVILAAMADEPLRRYIEAEANSR
                     LPGFHITIGAVDLHPLTLSVAVRDIVVRQDIRPDPPVVSIPNISADAELGPLLLGKIG
                     ADLRIEEPVFALTQKHVDGFLRKGDKEVVKEEAEAWQDRLRESMAFRGAFYVTNGHMT
                     YDDGKAASESLRVERFDIEATNITNRPGENEEYPSKLRVHANFPDQSQINLEGRADWF
                     AIPVPKVDADLKIERLQLKNLLPVAGRFNVQCREGVLDVSGHVQYSETSSVVAINQFQ
                     LDDAKIDYVHSAATKHKEVQRVKKAAETVKEAQHGSTMRVKVEQGKIRNSEIGFVNKA
                     TSPDYRVFLTDMEVELQNLSNRLEEGTGVVKITGKFMGSGPTVMNATFRPEKPRPDFS
                     LNAKIVKTNVSAFNDVLRAHGDMDIHQGTFAFFSEMTVKDNQIRGYVKPMLKDVEVYD
                     AEQDKDKALSKKVWEAVAGGVLGIFENKPRDEAATVTNLSGPVENPQANTWQVVAKLV
                     QNAFFKAILPGFERVA"
     gene            complement(27489..28169)
                     /locus_tag="VFH05_10935"
     CDS             complement(27489..28169)
                     /locus_tag="VFH05_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678778.1"
                     /translation="MDQRAHDVEEDLKNILQTRLALADKIDTLERRVQETVQTTKTAA
                     LDALDQAKNTAADWIESTTQQLNPSLQAGRRPWVMVGTAIAIGLLAGLAEQHRRGTRV
                     YPYYPPRAGGADVMPEKGQSDLPSGVYPFYGREAPRRSEGSRKPAGSRSDRPMGELLG
                     PLRDMWNELTDELAQERSRLQNTAIHAGRLFLQDVVRIAGQSLLDQLTRPERKAEHVS
                     GERRTLCE"
     gene            complement(28133..>28563)
                     /locus_tag="VFH05_10940"
     CDS             complement(28133..>28563)
                     /locus_tag="VFH05_10940"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6678779.1"
                     /translation="IEVMMQDRPRGTGFGLMNLVTGILDDAKLLMRQEAQLLRDEVKL
                     ELSKAGRAASGFGIGAVLGAVGALFLLLMLVHGLHEWTGLPLWGSYGVVGGMLAGVGL
                     TLILRARSLAGTVHTTPRRSLHTMKENVQWIKERMTLKRI"
BASE COUNT         5612 a         8740 c         7986 g         6225 t
ORIGIN      
        1 ttgccgatcg tcgcctcacc gatccccgcc tcctgggcga gccatacggc ggcccgcctc
       61 gccgcggccg gttgactctg ttccgtcacc ggaacacaca cttgtcggtc tgtcgcctcg
      121 ccgatcactt ccacctcaca atgcgcaccg tcgtgccttt gccaggcttc gattcgatcg
      181 caaattcgtt gacgagtcgc ttcgagccgc tcaaccccaa tcccaatccg ccgcgcgtgg
      241 tatagccgtc agtcatggcc agctcgacat cggcaatgcc ggggccctgg tctgtgaatg
      301 tcacctgcac accgcggcgt gtgccctcat ggaccgcctg caggcacatc tcgccgccac
      361 cgccgtgcac cagggtgttc cgggcgagct cgcttgccgc tgtcacgagt ttggtttgat
      421 cgaccaggga gaggccctgc aagacggcgc aatcgcgcac caactgtcgg gccttgatga
      481 tatcgtccgt ggaccgcagg gcgatcaatt catcgcgtaa gacggccatt ccccgacatc
      541 tttccagccg acttcagcag tgccatgccg gcatcgacgt tgagggcagt cttgatgctg
      601 ggcagcgcca gacccaattc caccagggta atggccaccg caggccgcat gcccaccacg
      661 acggtctcgg catcgagtac gcgcgacatg gacgcaatgt tgccgatcat gcggccgata
      721 aaggaatcga cgacatccac cgaagaaata tccaagagca cgccacgtgc gcgctccgcc
      781 acgatccgct cggtcagctc gtcctgcagc gtcatcgcca cgcgatcgtg catgtcgact
      841 tgaatcgtca ccaacagata ggggcccatc ttcaatatgg gaatgcgttc cattacttcc
      901 tcaattgtgg tcggcggacg ttgcactcaa accgggccac cggcattatc cagatgtgga
      961 ggcggtattt cgggtcacgg tggcccccag gcgctggagc gcaatcagga atgcatcggc
     1021 cagcgtggct ttggtgacga tatcgtttaa attgacaccc aggtgcacga tggtctgggc
     1081 aatctgagga cggatgccgc tgataatgca atcggcaccc atcaaccggg tcgccgcgac
     1141 cgttttcatc agatgctggg cgaccagggt atcgaccgtt gggacgccgg tgatgtcgat
     1201 gatcgcaatg cgtgcgcctg tctggacgac ctgctgcaac aggttctcca tgacgacctg
     1261 cgtccgcgcg ctgtcgagcg tcccgatgag cggcagcgcc agtacttcgt cccacaaccg
     1321 gacgaccgga gtggagagct ccattaactc ctgttgctgt ctcaggatga cggactcccg
     1381 agtctcctga taggcctcgg tcgtgtatag acccagctta tccagcaagg cggtcagatc
     1441 ccagagcatt tcagccagct ttttgctgtc ctgaccggcc cgttgctgag cgagatcgaa
     1501 caggggttgt ttgagtgaga agacgaaggt cgcggtctcg gaagggctga agccctgttc
     1561 ggcgcggctg cgggtcactt cggcgagatg ttccttgacc ggcttccaac tcggtgatga
     1621 cgcatccgcg ctcgccgaga acgcttcgga gaacaagtcc aaaaaacgcc gtgacatctc
     1681 ccgcaagtcc gattccttca cgcgatcgcg tctcagcgtc agggcggaaa gttgatagtc
     1741 catccagtgc ttcaggatgt gatcctgcct gtttcgaatt tcgtcagcca tctggctatt
     1801 ctgcgacatc ggcatcctcc cctttttgat gatgatccgc cacacagtct ccctgttcgt
     1861 ggttggcttg cacaccggag caagacacgc tccgcgcacg ccgagtgtca gactcagcgg
     1921 acctcggcaa cgccttcacg gctgccatcg gcaggctgtg cgtcgagaac ctcccgtatc
     1981 gtgcggtaga gctcgtctac tgcggcttcc ttcgtcagca ggacggcgcc gccggcattg
     2041 aggatcgccc tccgtgcttc ttcacctgcc tgcaccgaga gtccgatgac gaccgtctcg
     2101 ggaaagcgag ctttgatgca ggccgtcgct tcaatgccgt tctttttcgg catgttgatg
     2161 tccatcacga ccagtgcagg tcggagctgc tcgacgagct caatcgcttc ctccccgtta
     2221 gacccttctc ccacgattac tacgtcctcg taggattcca gcaggctccg caagccctgg
     2281 cgcaccatcg tatgatcatc aacgagcaac aaccggatgg tgttgccagg aggcggatgg
     2341 aacggcgcat ggggaatacg gtaaatggat tgttcagatt cctgtctggg ctctgttgcc
     2401 atggccgcca cgtcagatgg cttgccggcc gtcttcgcct tcgcaagggg cagcatcatc
     2461 gtcacggtgg tgccctggcc cggtatgctc ttgacctcga ggcgtccacc catcgcctgc
     2521 atgcgttccc gcacgctgaa cagaccgaaa tgcccgttct ctgatttggc ctccatcgcg
     2581 acaggatcga agccgcggcc gctgtcctgc acttcgatga ggagttgttg ctcctgatcc
     2641 accttcaagg ataccgtggc gcgatcggtc cccgcatgtt tggcgacatt gatcagcaac
     2701 tcccgcaccg attgaaacag caagaccgat tgatcgtccg gcagggacac ttgctcattg
     2761 aggagacgga cttcgaccag cagaccatgc aaaggcatct gcttggccaa ccacgtgaga
     2821 gccgacggca gacccaattc atccagcacc ggtggattca gcttagccat gagtgatcgg
     2881 gtgtactcgc cggccttttc cagcaggttg tcgacttccc ctaccagctg ctggatagcc
     2941 ggggcctgcc ctgagagttt ctgacgaagc tgtgtgacct tgattcgtcc caatgccagc
     3001 atctgcgcca ggtagtcgtg gagctccgag gccagccgtt tccgttcgcg ctgctccgcc
     3061 aatgtgagtt ctgttgccag cgcctgcagc cgttggttgg acgccacgag atcccgcgtc
     3121 cgctccgtga cacgctgctc gaggtcgcca ttcagccgtc gcagcgcttc ttcggcgcgt
     3181 ttgcgttggc ctaactccat ttccaacgca tggttggcca ccttcaattg tgcggtcctg
     3241 gaggcaatcc gaagttccaa atcggcattc aacgagacga tcgcattacg ggctgcggta
     3301 tgctcctcgc gtaatctccg ctgctcgagt gcgtgtcgca ccgcctcccc caaccgtccc
     3361 aggcggtcct tgagcagata gtcggccgcg cccgcccgca tcagcgccac ggcgtcctca
     3421 tctccgatgg catgggagac gataatgaac ggaattgcct gatcgcgttg acgggtgagc
     3481 tccaacgcct cggacgaact gaaatgcggg aggttgtgat cgtaaatgat cacgtcagga
     3541 gtgctctcct cgagtgcgat catgaacgcg tcccgtgtat caacgcggcg ggattccgat
     3601 tcgaatccgc tctttttcag ttcacggacg accaattccg catcggtcgc atcatcttcc
     3661 acgatcaaca cgcgcagcgt tgccatagcc cacactcctt ccgaggtcaa ttggattgtg
     3721 atgcggattc gagcgccaat tccgaaggaa actgattgag caatatccaa tacaagccga
     3781 cctgcctgac tgcatcacta aactgtggaa agtcgaccgg tttgacgacg taactgttca
     3841 ctccgagatt gtagcttttg acgagatcct gctcttcacg cgaagaggtc agcacgacta
     3901 tcggaattcc tttggtgcgt ggatccgact tgcatttttg taagacttcc aacccatcca
     3961 ctttgggcag cttgaggtca agaaggatga gcttggtgtt tcgacatgga ctgcgctcgg
     4021 cgtaacggcc ggttccgaac aggcattcca gcgcctcttc gccgtctcgc acgacatgga
     4081 ctctgttggc gatatgatgc ttgtgaaacg cccgtaacgt gatttcgacg tcctccggat
     4141 tatcttcgat caggagaatt tctgtaccat ttgtcatcgc aatgcctttc gtaatgagaa
     4201 aaagaaggtg gcacccccat cgaccttgcc ttccgcccac acgcgtcctc cgtgccgttg
     4261 cacgatgcgc tgcacgatag ccaagcccac gccggtgcct tcgaactcct ccttcaggtg
     4321 taatcgttgg aacactccaa acagtttgtg gacgtatttc atgtcgaagc cgacgccgtt
     4381 gtctcggaca aaatacaccg aggcgtcagc ctggcggtcg tctggcagcc acccgacctc
     4441 gatctgagcc acggggcgat ggcgcgtaaa tttgaaggca ttggccagga gattcatcca
     4501 tacgagcctc aacagccgcc agtctccctg gcagggcggc agatcaccga tcgttaaatc
     4561 aacggtgcga ccgtcgtgcg tcacgttgag ttcatcggcc acttcccgtg cgatctcggc
     4621 catcttcgtc ggacggaaat ccatgccgat gcggcccaag cgcgagaatt ccagcaagtc
     4681 gtcgatcagt tcaccggctt gaccggcagc cttcctgacg atcccaaggc agcgccgggc
     4741 ctcggagttc aagtgcgcgc catggtcttc ttctaagatg cggatgtacg cgtcgatcgt
     4801 gcgaagcggc gcacgaagat cgtgcgacac agagtaggag aagctttgca gttccttatt
     4861 cgcgtcggcg agacccgatt tctgttgagt caattcgtcg ttcgcgatcg tcagcgcgcg
     4921 gtcacgatcc ctgagagctt gctcggccct tttttcctcc gtgacgtcac ggaagacgac
     4981 gacgaggccg gcgatctcct cgcccgcgcg aatcggcatc gcactataca ccacgtcgaa
     5041 gcacgtgccg tccttgcgca cgaagacatc ctggtggttg atcaaggacg tgcccttctg
     5101 gagcacctgc aactccgcgc actcctgtgc aggaaagggc gtcccatcac gatgcttgta
     5161 atgggtcacg tcatgcattt tgagtccgag caattcctcc ttgttccagc cgaacagctt
     5221 ttccgcagcc tgattcatgg aaacgacgag tccatgccga ttgaccgtgt acagcccttc
     5281 acccatgttg ttgatgaccg cttcctgata ggccaaggcg tgccgtagcc gttcacggct
     5341 ttccttcaac gcccgctcct gttcaacccg gggagtgatg tcccgggcaa tcttcgatgc
     5401 gccgatcacc tttccgagcg tatcgaacag cggcgaaaac gtcaacgcaa cgtgcacatc
     5461 gcttccgtct ttgcgccgcc ggatcgtttc gtagggctcg accgtccgcc cgtgcgaaat
     5521 cgcgtcgaga atgcggcgct cttcatcctg tctatcggct ggaatgatga gggtcacggg
     5581 ccggccaatc atctcctctg cccgatagcc gaacagtcgt tccgccgccc ggttccaact
     5641 cgtcaccatg ccttgtagat ttttgctcac aatggcatcg tccgacgatg cgacaatggc
     5701 cgctaaatgc gccgtcgccc gctcagaccg ttggcggtcc gagatatccc tgagataggc
     5761 tgtgaaaaac ggagggcagc ccggtcgccg agtcacggca atagagaact cgaccggaat
     5821 ttcggacccg tcggcccgga gcgcgggcat ctgaatccgc cgtccgatca cggacgcctc
     5881 accggtctcc aaataccgtc gtaatccgac tttatgactc tcccgcaaac gcggcggtat
     5941 gatcaggtcc gcgaccggac ggcccagggc ctgcgcctga gtatagccaa acgtcttttc
     6001 agccgccgga ttgaaatcct ggaccagtcc gtcggcatcc atcgtaatca aggcgtcgag
     6061 cgacgattca aaaatcgcac gtgtccggtc ttcgctttct ctgaggtgat catcgatccg
     6121 cttgtgctcc gtcacgtcaa tcagcatgtt gaccgcgccg atcacggtgc cggaggcatc
     6181 acggatgggg tctggatgag gcatcacgtg ccgcttggat ccgtcaggcc gttcgatgac
     6241 gatttcctct ccgcggacgg cccgtccttc ccgcagggtg accgccatcg gaaattcgtc
     6301 caagggcaac ggagttccgt caggccgata gattttccat gacccgcacc agagatcctt
     6361 tccgatttcc ggctcacgcc cccataagct caccgccgcc tggttgtaga gctgaatccg
     6421 cccttgggcg tcgcaggtat agacggcggc ggggaggctt tgaatgagag cccggtaccg
     6481 actctggctg agccgcaagg cttcctggtc ctgctttcgt tcagtgatgt caaaattcac
     6541 accggtaaga cgcaccggtt gccccgtctg atctctgagc caccgccacc gcccagccaa
     6601 ccaccgaatt tctccgtcgg gtctgacgat gcgaaactca cgatcttccg gctcgccgct
     6661 ccgacgcgat cgttcaacgg cctccaccat ttcgtggcgg tcatcaggat ggagacaaga
     6721 ctcccaggcc gcctgggtct tgtcgaactt gtccgggtga agaccgtaca tggcatagag
     6781 ttcaggactc caactgttca cacctgtgtc aaggttccag tcgaacgagc ctacctgaga
     6841 cgcttgttgg gccagcgtca gccgttcttc cgtgacacgt aggccttgag ccagttcttc
     6901 ggcccgtcgc cgggcccgat gcatgacctg tccgaccgca atgaaggtca gaccgacgcc
     6961 tccgtaggcg acaagaccga cttgttgaga caggccggtg atgtcgaatg aaaatcgcgg
     7021 tgggacaaaa aaccagaccg cagcaactcc gcctagcaac gtggcggtca acgcggcaga
     7081 cactcccgca taccatgtgg taaacgcaat ggacacgaag aaggtgacgt aaggaagatg
     7141 atcgccaaga tacggatcaa gaacaagacg gcaaccgagt gccagactcg tcgcaaacag
     7201 tgcgacaaga tgttctttga tgaccgcggt caatccgctt tgtttcaaat gcccaccaat
     7261 accggcctcg aaggtcacac cacttgcctc ccggtgagtc accaggctcc gtaagtgatt
     7321 ggggaaacga gggaagtata ggggatttta gacaagaggc acccctaggg ggacccctag
     7381 tttttggtgg atgtcagcaa aagcaagagc gaaccggagc ggctctgcag aagaattcta
     7441 tttcaacagg gcggccggta cacgtccatc atttcaatgt tggttgtggc aggataaaac
     7501 tccccgaacc gattcatcac ccacccctta tgggcatgca cctctttgag cagtatcggt
     7561 tgagcgacgt aggtcgggca gacaggagag atcacgacga attgatgaga cgcgctttcg
     7621 cgttcgctcc aatagcagtc gaagacctgg acctgcaccg cctgatcacc aagcatcgcc
     7681 tggtccggcg gctctgcacc acaggtcatg ttgccgttca ataccagcat aagaaacaga
     7741 gcaatggcgt tcatacgcat ctcctctttg aggctcactg caccgcgacc aattctccca
     7801 tgctcatcgc gccattcagt atcgcgtgct tgggggctcg cccctacatt cctgcaagaa
     7861 gagttccggc atgaatgttg agcactacgt ctgcgagagt gatgaaagaa ctgattaata
     7921 gcccaaaccg gggacgacga tagcggcgcc tctatacaga ccgggacaaa ctgtccgccg
     7981 tgaggccacc tatccttttt cgctaacaca tagtatctag cctcaaatgc ctagtgagaa
     8041 cgtgacgtga aaaagacgac gcgtgtttga ctcgggtgaa tcctcactgc tttcaggggt
     8101 tccccctgta aggcaagccg gacgaatcgg tcaggatcag ttgtgactag gaaagcgctt
     8161 cccaacgagc cgtcatccat cacccggcgt caaagccggt cccacgcgat tgagtttggc
     8221 tcacgtcgtc cggacctgcg atccgtttca ttgaccggtc tgttcatcct tgccctcgtg
     8281 tggttcctgc atcaagctca ggattttgcg gttcccctga cgctggccct gctcctgcac
     8341 tttttgttta tgccgatcgt gcgctggttc aaacgtcatc acgtcgtgga gccggtggcc
     8401 gccgccatca tcctcctggc catgacggga ctggtgacca tgacgatgta tgaattggcc
     8461 gcgcctgcgg cgcagtggct cgaaaaagca ccacgcgcgt tccgtgtgat cgaaagcaag
     8521 ctgctcccgg tcaaggaatc ggtgtctcaa ctctccgaag ccacggaggc catgaaccgt
     8581 gtcggacaaa ccgactcgtc tcccgccgtc cgcaaggtgg aagtggtgcc ggaccggcga
     8641 gcggcagtcg tgagcctctt gggaaaaacc atggccggga tcgggacgac ccttcttctc
     8701 ttgtacttct tattggcgtc cggggatctg tttgccaaaa aactcctcac cgtcttgtcc
     8761 cggcgcgagg atcgtggctg tgccgccgac attcttgaag gccttgagcg acaaatgttc
     8821 caatatttgt ttcaaactgt cgtgattcag agcggatttg ggtttgtctt gtggatgtct
     8881 ctttgggcac tggggatgcc gaatcctggt ctgtgggcgg ccgcagccgc gattttgcaa
     8941 atcgtcccgt acattggtgg tctcgcggta ctggttctca ttgccgtggt ggcagccgtg
     9001 acgtttgaca acacgtggtc tatcgccggg cccgttatcg cctatctctc gttgaccaca
     9061 ctcaaagggt ttgttgcgcc tgttctcatg gggcagcggc tggtcctgaa cccggtcgcc
     9121 gtactggtga gcctggtatt ttttggatgg atgtggggcc ttgctggaac gctgctggcc
     9181 gtgcccctcc tcgcctgctt caagatcatg tgcgatcaca tcgagagtct ggaaaggaca
     9241 ggtgagtttc tcgcctgacc aacaccacgc cacgaatagg acgacaggac acgccagtca
     9301 gggagccgcg gtcgaagaag gcccccgtga aggagccacc cccacgccag cctcagaagg
     9361 gtcctgtcgg cgatcctccc acgaagcgac agccagaaaa aaccagagcg tgaacagcac
     9421 gagcgcgagg gagagaagaa aatggctttg aagacagggt tacgccgcga acctgagagt
     9481 ctggccgatc tcaaacctga acaagcgtgg ctggtgctca ggccgcctca ctcccgtaat
     9541 gcttgaacag ctgcacttca taggcgggcg tgatcagtct cgagggatca tagcccggcg
     9601 cggattcgat cgcctgttgc gaaagcataa cggcaaccga tcgctccgcc cagctgatat
     9661 ggtcgatgcg catcgtctgc agcaggacct ttttgccggg caaccaattc ttcgtatcca
     9721 cttcgacata ccggactgtc cacgcctcat catccacgac caggtcctcg acgtggccga
     9781 tcgtcccgtt tttggcctgg atgtcgtagc cgatgacctc gctcgtgctg cgcaggtgcg
     9841 gattcttggg cgcatccgca ggaagcgtcg tatcgatcgg gatcggtggc gtttccggaa
     9901 atgggagtga gcttccgagg gcggtcggcc ccggctccca atagggagcc cactgaaaat
     9961 gccggaagta ggcctcttcg tattcccgtg agagcggctt cgcggcttcg atcgaaggcg
    10021 cggctttgat ctgctccttc gtgagcgcga tgctcatggt tccgttggcg tcattgatgt
    10081 cggcgaccgc cgcaggagcc agcaggactt cgcggctcaa gagccacccg ccggccttca
    10141 ccacgaggta gcggaccgcc caggtgcgat cgtcgaaata aagctcctgc acattgccga
    10201 tctccccgtc ggtagccaga agcgtcaaat cagtcaactc ccgtatgcga cgcagttgct
    10261 tcatgacaag tgttcttgag gaaaaacagt ggacggcggc gccaggttgc ggctggtttt
    10321 cgttccgcgg ttcttataga gaatgccggc gatgatgaac agataggaga gaatcctgat
    10381 catatagccg aaaacctcca taccgaacgg gggaatgctt tcgtctacca ccacgcggct
    10441 gacggcgatg gccaggaacg aggccgagaa aaacaagaaa aaccggtcgc cggtcatttt
    10501 ccaaaaccgc acgaaaaaca gggcggcaag gacatccccc ataatcacgg cacccagtag
    10561 aatatgtgcc agcggtgtca tacttccttt tccagaatca gtgccggcaa cagaatcatg
    10621 agaccggtca gggccaccgc gtagcggtac accatcaagt cgacatcggg gaacacgact
    10681 ttgtccagga ccagaaagac attgttcatc gcggcgatac agaaaaacac actgcaccag
    10741 aacaacaggc gataccgcgt cttgatgtag gcctgcagca ggagccacgc gcaggtcagc
    10801 gcggtgagca tgcagaggct atagatgacg atcggggcaa taatcatttt ccgtctttct
    10861 ttttcaacgc gaacgcatcg gcgaactcgt gaattcgtgt ccgaggcttg ccatgaatca
    10921 ggttcgaaat aggcacgaga tgatgggagt agtagtacac gagccggtcg acgaattccc
    10981 ggagctcacc gtttgacggg gcgtagcgat acgaccagcc gatgtcctct tctctcgcca
    11041 ccaccagccc attttgacag agcccatcca gttcactccg acaggtctgg tcggcgatgt
    11101 ataaacgctc ggccaacgtg ctcgacggcc accaggtgtc tggcgcgttg cgcatcatga
    11161 ggatcgcttc gagctgggcg atggagtcga tgcggtccag gacgaatcgc tggacctcag
    11221 aaggaacgac cgcatccatg ggatgcttgc gggacgtttc gccttccaag ttcactccgt
    11281 gagggcggac gatgaaaaag acgcactgct cccggtcaaa ctggccgcgc ggcatcgtaa
    11341 aatggtttca atcaatcgct cggctgcggc atccttcgtc aagtaggcgg cagctccgga
    11401 ctctctcatg gcggcttcgt ggtgtccgcc ctcatgcatg gacaggccaa tgaccaccgt
    11461 cgaaggtcgg tgttgcttga tgcgtcttga ggcctccacg ccgtccatct tgggcatgtt
    11521 gacatccatg accacgacgt caggcttcaa ggctgcggcc agcgcaacgg cttcctggcc
    11581 gtcggcggct tccccgacga cctgcagatc cgtgttgtac cgcaacacgc tcgagaggcc
    11641 gtttctcacg agcgcatgat catccaccag caggacccgg atgcggagtc gctcttcgag
    11701 atgtgatgcc gacgagacca attctggagc gctccgaaac gctgatcgtt gggccgtcaa
    11761 ttgttgagca tactcctcgt cgcttggccg ccgacggtcc gtcatggtct cccctgttgt
    11821 cacaggtgga gcctcggctg gtgcgtcggc tggcggaaga ggcatggtga gcgtcgcgat
    11881 cgtgcctttg ccgggcgagg aggtaaaatc aaatgcccct cccagcgcac gcatgcgttc
    11941 gccgatgctg aacaaaccga atttggaaga ccgtgcgccg gatgaggacg tgagcgtatc
    12001 agcacccagg ccctcgtcga cgaccgcaat gctgagttgt ccatcctgcg gttgtatgcg
    12061 gatcgtcgcc tgatcaacgc gcgcatgttt tgccacgtta ataagcaact cgcgaatgga
    12121 ttgaaagatg aggaccgcct ggtcttcagg cagcttgacg gacaggcctg cctgaacgtc
    12181 cacgtccact gacaggcggt actgttggcg catctgatcg cccagccatt tcagggcagc
    12241 cacgagtcca aactcatgca acgccatcgg cgtcaactgg gccaccagcg agcgggtata
    12301 cgttagggcc tgctggagga tgtcgtccga ttcgccgagc catccttcca cctccgcctc
    12361 agccatccgg ccgcgcgatt gggacacttt gaggcgtgca cagaccagca actgcgcgag
    12421 ataatcgtgc aaatcggtgg caatcctgcg ccgttcccgc tgttcggcaa tggtcaattc
    12481 ggacgtcagc tgacgcaaca agctctgcga ctgcaccaac tccttggtcc gctccttgac
    12541 gcgatcctcg agaccttccg ctaaacggca cactttctca tgcatcgccc ttcgctcggt
    12601 gacgtcacga ttcgtctcca gcacgagaat gccgccgtac tcctcggtga gagtcaccat
    12661 gcgactttca acgacgacgg actgcccgcc tttggtcaac tgcctgatct ccccctccca
    12721 gcggccatct tcgatcaatt gccggtggaa ctcctcttcg gacagcggca aggccgttga
    12781 gaggagttgg tggatcggct ggcccgccgc ttcagcgacc ttccatccgt acaattcctc
    12841 agcgccgtga ttccaataga tgacacctcc gcccatccgc cacacgacca ccgcatcgtg
    12901 tgtctgcgcc agaaggccgg cgcgccaccg caattccgct tccgttcgct tgcgttcgga
    12961 gatgtcccgc aaaatgcctg tatagcggat ctcgccgccc gaaaaacttt cgctgacggc
    13021 aatttccaca ggaaaggcgt cgccctgccg gcgttgcccg tttaattccc gccagaggcc
    13081 gatcagccgc gtcaaccggt tcgctgcgtc cgcggacgat tcctcagccc cctgagcgaa
    13141 ccaggcaggg atgagcatgg agatactacg tccgaccatc tcttcgaccc ggtagccgaa
    13201 catctgttcg gtagccggat tgcacgagtg gatcacgccg gcgtgatcga acgtgatgat
    13261 gctgtccatg gcggtttcga ccagcttgcg ataccgctct tcgctttccc gcaagcgccg
    13321 ctccgcctga tgcttgtaga gcgcgatttc aatctgagtc gtgacgtcac gctcgtcgaa
    13381 gggcttgaga atgtatccga acggttccgt tgctgctgca cgccggagag tggccatgtc
    13441 tgaatgggcg gtgagataga caatcggaat gctgcggaat gcctggatgc gttcggcggc
    13501 cttgatcccg tccagggaac cagccaagcg gatatccatg aggacgaggt cgggccccag
    13561 tgctttcgcc gtttcgatgg catcttcacc gtcagatgcg acggcaatgg tgcgatagcc
    13621 ggctcgttcc agcttgccgg ctaaatcggc cgcgacgact gcttcatctt caacgatgag
    13681 tatgttcgcc ttgggcatcg agcaccgcct tttcgtctga ggaaccgccc cgtgtgttct
    13741 gaagctggaa gtgcaattca aacgtggtgc cttcacctga atgcgtcttc acttcccccc
    13801 gcagctgctt gctcaacatg cggatcaggc gcaagcccaa cgactcggcc gcgtcgaggt
    13861 tcagaccggc aggaaggccc cacccgtcgt cccgaaaacg cagacgcacg gattcattcc
    13921 gcacactggt caattggatt tcgatcgtgc cgcgttcccg tccgacgaac gcatgcttga
    13981 ggctgttggt cgccaactcg ttcagaatta atccgcatgg aatggccatg tccacaggaa
    14041 ggaagaccgg ctcaagttgc agctgcaagg tgacctttga cgacacttct ccatgcgcca
    14101 ggaagagttc cttcatgacg ttccggatgt aggcaccgag ttccgtgtga gcaagatcgg
    14161 cggattgata cagtttctcg tggaccagcg ccatcgatcg gacccgatcg cgcgtgtcgc
    14221 ggaagagcgt ccgcatggca gggtccgtca aacgttcggc ctgcaaatca atgagactgg
    14281 agatgacttg gagattattc ttgacgcgat ggtgaacctc cttgagcaaa atatccttct
    14341 catggagcga ggctttgagc tgcttgagcc gctcctcgat ttcgacttcc atcgcccgat
    14401 tcatcgtggc aagggcccgg gtcttacgaa agaggtccac gaagaccgcg accttcgatt
    14461 tcaggatgag cggattgacg ggcttgctca aatagtccac cgcccctgcc ccataccctt
    14521 ggagcacgtc atcatcttcg cggtagtagg cggtcaggaa caggatggga atatgctgcg
    14581 tccgttttcg ttgcttgatg agctgcgcga gttcataccc gctgacctcc ggcatccgga
    14641 cgtcgaggac gatgacggcg aactcttcgg cgatcagtgc cagtaaggct tcgtcagcag
    14701 tgcgggcgcg gaacaggcga taatcgggac aagccagaat gctgtcgagc acgtcgaggt
    14761 ttctggaatc gtcgtccacc agcaggatat ttgcgggctc tttgaaagtg gtcatagcgt
    14821 tagtcatccg ttccttccat gcaagaacat cctcccatca cgctgccaag cccgctgaga
    14881 ctgagtgtac gtgccaggtt tttaccattt caacccggcc ctcgtgcgac ctccctaccg
    14941 gtacaaccac acccgtagca tggacagcag ttgctcggta ttgaccggtt tcgcgacgta
    15001 atccgaggcg cccgcctcca aacacttctc gcggtcgccc ttcatggcct tggcggtcaa
    15061 ggcgagaatg gggagcgagc gaaactccgg ctttttccgg atttcgcgga tcgtcgtata
    15121 tccgtccatc tcgggcatca tgatgtccat caacaccacg ctgagatcgg gcgtgttctc
    15181 gatgagttcg atggcatgac ggccgttagt ggcgctcatc acatccattt cctggttttc
    15241 gaggacggtg gtcagggcga aaatgttccg cgcatcgtcg tcgaccacca ggactttatg
    15301 gccatggagg gcttcattgg attggtgtag tcgctccaat aattgttgct tcggttccgg
    15361 cagatccgcc acgacccggt gcaggaacaa cgccgtctcg tcgaacagcc gctcgggtga
    15421 ttgaacgtcc ttcaacacca cgctcttcgc cacggccttg agccggcgtt cttcttcggt
    15481 gctcaaatct tttccggtga acacgacgat gggcacatta ccgagcgtcg gatccgcctt
    15541 gacctgatca agcagctcaa agccgctcat gtccggcagg cgcaaatcaa gtacgcaaca
    15601 gtcgaacgat tggctgcgga gcgcctcgag cgctcccgct cccgtgccca cattgacgat
    15661 ctcgatgtcg tcgtgcagga gcagctccgt gatgctgcgg ctttccagct cgttgtcctc
    15721 gaccaccagc aaccgcttga gatgcggcgt gatgaagttc cggatccggt cgaagcagga
    15781 ctcgagtccc tcggtcgtga ccggtttgac catgtaggag aaggcgccgt gggccagccc
    15841 gtgctggcgc tcctcctcca acgaaatgat ctgaacggga atgtgtcgtg tcttggggtt
    15901 cagcttgagg ttgttcaaga ctgtccaacc aagcatgtcg ggcagaaaga tgtccagggt
    15961 aatcgcagcc gggacgtact cgagtgcaag atccagcgcc tgcttgcctc tggtcgccac
    16021 gatccctttg aagcctttgt cccgggccag ccccaagagg acccgggcgt agtgcggatc
    16081 gtcttcgaca atgaggacca caagctcgcc gtcctgtacg gtatcccggt cgtctgcgat
    16141 cttttcttcg ccaaccacgg gcaggatcgc cagcgccggc gcttgacccg ggagagcgac
    16201 ggcagcggcc gacgggcgga cgcccgcgct cgagggaccc gtgtagacca gcggcagaaa
    16261 caacgtgaac gtgctgcctt gcttgggttc gcttgtcagc ttgatctcgc cgcctaaaag
    16321 ggtcgccaat tcgcggctga tggccagacc taaaccggtt cccccatatt tgcggctcgt
    16381 tcccgcatcc gcctgttgga aggcctcaaa gatcagcttc tgtttttcgg gcgcaatgcc
    16441 gatgcccgta tcggaaatcg cgagggccat gaccgtatgc gaccggctca acaccggatg
    16501 ctcggtcgtc catccttccg ttgcaatccg gacgttcatc gacacatgcc cgtgagacgt
    16561 gaacttgaag gcgttcgaca ggaggttttt gaggatctgc tgcagacgtt tggggtcggt
    16621 ggtaaacgcg cgaggcaatc cgctctcgaa ctccagatgg aacggcagat tcttttgttc
    16681 ggcgatgtgc ttgaagttgc gctcgacgct ttcgcggagt gcgctgaacg tcatctcttc
    16741 ggcctcgacc gtgaccgtcc cggactcgat cttcgacagg tccaagatgt cgttgatcag
    16801 gttcagcagg tccgaaccgg cgctgtgaat gtttttcgcg aattcgacct gcttctgcgt
    16861 caaattgccc gtcgtattgt cgccaagttg ctgcccgaga atcagaatgc tgttcagcgg
    16921 ggtacgcaac tcgtgcgaca tgttcgccag gaattccgat ttgtatttcg aggtgagcgc
    16981 gagctccgcc gccttttcct ccagcgcgcg gcgtgcctgc tcgacctctt tgttcttgcg
    17041 ttcgacttcc gtattgcgct cggccagctg cttggccttg cgctcgagct cctcgttcgt
    17101 ttgagtcaac tctttctgct gcgattgcag ttccgacgta agctgctgcg actgttgcag
    17161 caggttttcc gttcgcatcg tggcttcaat ggtgttgagc acgacgccga tgctttgcgt
    17221 gagctgctcg aggaaggtga tgtgggagac cctgaacgta tgcaacgacg ccaattcgat
    17281 gaccgccttc gtctgtcctt cgaacaacac ggggagcacc acgatattgg ccggcaacgc
    17341 ttcgcccagg ctcgagtgga tcacggtgta ggagggcggc acgtcggtca aaagaatgcg
    17401 ctgcttttcc acggcgcatt ggccgatcaa tccctcaccc actcccacac gctccggtgg
    17461 ttcgcgatga ctcgcgtagg aggccagcag acgcaacgag ggcttctgcc cttccacgat
    17521 gtccatctga tagatggtgc cttcctgcgc ttcgaccagc ggggccagtt ccgaaagcag
    17581 catttggccg accgtaaaga ggtcccgttg tccctgcaac atgcgggtga acttcgccac
    17641 gttcgtcttc agccagtcct gctcctggtt gcgctccgtc gtgacgcgca gattgtcgat
    17701 catcgtattg atgttgtcct tgagctctgc gacttctcca cgcgtttcca cctgaatgga
    17761 ccgcgtcagg tcgcctttcg tcaccgcagt cgccacctcg gcgatggcgc gcacctgcgt
    17821 ggtgagattg gctgccaaga ggttcacgtt gccggtcaaa tctttccacg tgcctgcggc
    17881 gccgggcacg ttggcctgac cgcccagccg tccttcgacg ccgacctcac gggccacgtt
    17941 cgtgacttga tcggcgaacg tggcgagcgt gtcggtcatg ttgttgatgg tttcgcccaa
    18001 ggcggccact tcgcccttgg cttccacggt caaccgctgt ctcaaattgc cgttcgccac
    18061 cgacgtcacg accttcacga tgccgcgtac ctggtcggtc aagtttgtcg ccatgacgtt
    18121 gacggagtcg gtcaaatcct tccaggtgcc ggcgacgccc tggacgacgg cctggccgcc
    18181 cagcttgccc tccgtcccga cttcgcgcgc cacacgtgtt acctcggcgg cgaacgcgtt
    18241 caactggtcc accattgtat tgatggtgtt ctttaactcg agaatctcgc ctttgacgtc
    18301 gacggtgatc ttgcgcgaga ggtcgccgcg ggcgacggtc gtcgtgacct cagcgatgtt
    18361 gcgcacctgc gccgtcaggt tggcgcccat ggcgtttacc gagtcagtga ggtctttcca
    18421 ggtgccggcg acgccgggca ccacggcctg aacgcccaac cggccttcgg ttcctacttc
    18481 gcgggccaca cgggtcacct ccgacgcaaa ggagcgcaat tgctcgacca tcgtattgat
    18541 ggcttccttt aactggagaa tttcgccgcg cacgtcgacc gtgatcttac gggacaagtc
    18601 accgttggcc acggcaatcg tgacctcggc gatgttccgg acctgggccg tcagattgga
    18661 cgccatcgag ttcacgttgt cggtcaggtc cttccaggtg ccggcgacac cgggcacgag
    18721 agcctggccg ccgagcttgc cttccgtgcc gacctcgcgc gccacacgcg tcacttccga
    18781 agagaaggcg cgcagctgtt ccaccatcgt gttggccgca tccttcagct gtaagatctc
    18841 gccgcgaacg tcgaccgtga ttttctttga gatgtcgccg ttggccacgg caatcgtgac
    18901 ctcggcaatg ttccggacct ggtcggtcag gtttccggcc atctggttca cggagtccgt
    18961 caactctttc cacacgccgc tgacgccttt gacttgggcc tggccgccga gcttgccttc
    19021 cgtgccgacc tcgcgcgcca cccgcgtcac ttccgaggcg aacgaacgca attgctccac
    19081 catgctgttg gcggcttcct tgagctgcag aatttcgccg cggacgtcca cgatgatttt
    19141 ctttgagagg tcgccattgg ccacggcgac ggtgacttcg gcgatgttcc ggacttgcgc
    19201 tgtgaggttg ccggccattt cgttgacgct ttccgtcaac tctttccaca cgccgctgac
    19261 gcctttgact tgggcctgac ccccgagctt gccttccgtg ccgacctcgc gcgccacacg
    19321 cgtcacttcc gaggcgaacg aacgcaattg ctccaccatc acgttgatgg tttccttcaa
    19381 ctgtaaaatt tcaccgctca cgtccaccgt aattttgcgt gagaggtctc cgcgcgcgac
    19441 cgacgtcgcg acttcggcga tgttccggac ttgcgccgtc aaattattcg ccatgctgtt
    19501 gacgttgtcg gtcaggtctt tccacgttcc gccgacgccc gggacgaccg cctgcacccc
    19561 gagcttgcct tccgtgccga cctcgcgcgc cacacgagtg acctcggcgg caaatccgtt
    19621 caattggtcg accatggtgt tcagcgtgtt cttcagctcg aggatttcgc ccttcacgtc
    19681 caccgtaatc ttgcgcgaga ggtcgccctt cgcgacggca gtcgcgactt cggcgatgtt
    19741 ccggacctgg gccgtcaaat tgacacccat cgcgttgacc gagtccgtca ggtctttcca
    19801 cgttccgccg acgcccggga cgaccgcctg caccccgagc ttgccttcgg tcccgacgtc
    19861 gcgcgcgacg cgcgtgactt cggccgcaaa tccgttcaat tggtcgacca tcgtattgat
    19921 ggtgtttttc aacgccagga tttcgccctt gacgtccacc gtgatcttgc gtgacaaatc
    19981 gcctcgcgcg acggcagtcg tcacttcagc gatattcctg acctgcgccg tgaggttgtt
    20041 ggccatggcg ttcaccgagt cggtgaggtc tttccatgtc ccgccgacgc cgggcacgtc
    20101 cgcgttcacc ccgagtttgc cttcggtgcc cacctcgcgc gcgacacgcg tcacttccga
    20161 ggcaaaggag cgcagctgct ccaccatcgt attaatcgtt tctttgagtt gaagaatttc
    20221 cccccgcacg tcgacggtaa ttttgcggga gaggtcgcct cgggcgacgg caacagtgac
    20281 ttcgacgatg tttcgaactt gggcggtgag gtttgacgcc atcgaattca cgttgtcggt
    20341 gaggtccttc caggtgccgg cgacaccggg cacgaccgcc tgcccgccga gtttgccttc
    20401 ggtgcccacc tcacgcgcga cacgggtcac ttccgaggca aaggaccgca gctgctccac
    20461 catcgtattg atcgcctctt tcaattggag gatttcgccc cggacgtcga cggtaatttt
    20521 cttcgagagg tcgccgttgg ccacggcgat ggtgacttcc gcgatatttc gcacttgtgc
    20581 ggtcagattg ccggccatct gattgactga atcggtgagc tctttccaga cgccgctggc
    20641 gcctttgact tgggcctggc cgccgagctt gccttcagtc ccgacttcgc gtgcgacacg
    20701 tgtgacctcg gaagtaaaga ccgccagctg ctcgatcatc ttattgacga gttttgcact
    20761 gcgaaggaat tcgccttcga gcggccgccc gtctttctcc agtgccatgg cctgactgag
    20821 gtcccctttt ccgactgccc cgacggcacg ggtgacctcg gtcgttggag acgcgaggtc
    20881 gtcgattaac gtgttcagtg actcaatctc atccgaccac cctccagtga caccaggcac
    20941 cgacagccgc tgccgcaacc gcccttcctt cccgacgacg cgagaaatac ggacgacttc
    21001 ttctgcccgc ctttggctga cagaaagaat atcgttcatc gtgtcggaca ttttcccgag
    21061 cacacccgtc catgtggccg gcaagcgcac ggagaaatct ccgttccggt acgccaccat
    21121 gaccttaaga atttcgccaa gttgctcgtt gatgtcatga cggcggcggg taccaccatt
    21181 ggcggcaacc ggcgcatcct cgagagccgt ggtttcttgc tcatcgttct ggtgtttcat
    21241 cgtgcctccc ggcgggcaaa atcggtagaa cggtaacccg ttggtctata tgaaccgagg
    21301 caacgtaccg cgagggaaga gaccttgcta actagggaga cccctagtac ctaagtgcat
    21361 acagtcatgc gcgttctacc cgatgcggac gacgtaaagt acaagtcagt acagcagaat
    21421 tttcaccgtc gcaagcaaga gggacagcta gccttaaccc gagaaaccca tccagagccc
    21481 tatgcatgga atgcgctgtt tccgctgtaa aggatcggtt gcaatgtcct tcgagctttc
    21541 aaagacttgc atcgaagaga gaggcatgtt gagccagcat taacgaagtc cgggctgacg
    21601 ctgagattca atcgagagca agcggacacc agatggcgtt tggaagggaa aactgaaaac
    21661 aagaccgttg tccctgcaac gcgaccagga tcgactgttc acgaaacccc gtcttcctcc
    21721 aacgcttggc gaagcagaac ttcacgcagc aacgtttcca ggtcttgatg catcaactga
    21781 atgctggatt ccaattgatg cagcatcgcc tctcgcacat gtaaatcttc gctgtcatag
    21841 agcccgcctt ctccgaatag gagagccttg cgctccttct cctggttcga accgggcgca
    21901 cccaatcgtc cttcttcgtt ccaggtctga acaagtacat cgcgcaggct gtgcccctcc
    21961 agatcacgga tgtccggttc agtcaggaac cgccagatgc cgggaggaaa aaattccttt
    22021 tccttgccct cgccgttcca aaatgcctgg agatgattgt gggggtgggt gaattcctgc
    22081 cggaccttcg tgaaattcgc ggacgtaccg aaaagaactt ccaatgtccc gccggcgacg
    22141 gatgcgatgt ctgcggcatc ccccgctctt gttgccatgc cgatacctcc agacaggatg
    22201 ttcgctaatc cgcccgcgat gatgccgaga atcgtctgcg tcgtcgttcg tgtggtctgc
    22261 acttgcttga ggcggtcttg tacctcatca gcacgttgaa cttcacattc tatctcggcg
    22321 actagactcg acacttccat gttggcgagc tggagccgtg tcgtcaattt tcgacgggcc
    22381 cgttcgatct ctgcagagga ggcatgctga ttttccagac gcgcgagatg gttcagcagc
    22441 ggtagcagat ccatgacgtc ggccacatgc acggcgatgg tcgagtagag tttgacggcc
    22501 tcttgccgtt cggccgacag gctggttgca gccggcttat ccatttcgct gagcggcatg
    22561 cggcgaagat ccatcgaagc cggcagacag cggcgatcaa acgaggtcgc tttccccggt
    22621 ggcaatgtcg aggcacaggc ggtgaggaga agagaagaaa ccgcgaccag acagagcggg
    22681 atggatcgca tggcgcgtcg atcgctcctt catgccgccg cccggcaagc gtcctgcccc
    22741 aaccggcgat gaagcagggc cagcaccgct gcttcttccg gcggaaggtc ccgcacgtgt
    22801 gagcgaagct cccggccggt tcgtcgattg agcgacgccg cgagcgtccc gtcgaaatac
    22861 gcgttgacga tgtcgggatg gacatagcac ttccggcaga cggccttggt gttgccaagg
    22921 cgtttggcca cggtttcgat ggccctaagc acgttacgtt tcgcttgcgc ctgggaatca
    22981 accgcactga attcccggag tgcgtaagcc gcaaggacgg tgccggccca cgtccgaaaa
    23041 tctttggcgg taaactcatc gccggcgatg gtcttcaaat aggcattgac gtcggccgcg
    23101 tcgatcgtcc gtctctgccc ggtctcatcg aggtactgaa atagttcgaa tccaggaagg
    23161 tcccggcttt ggcgcacaat tgacgccaag cggcgatcct gcagatcgac ggcatgaagg
    23221 atgccgcttt tgccgtggaa ctgaaaccga agggcggaac cgttgacccg gacatgacgg
    23281 tcacgcatgg tggcgagacc gaacgaatca ttcttcttgg cgtattcctc gttcccgacg
    23341 cgaataagcg tgacctccaa caaacgcacg acggtggcga ggatcttctc tctgggaagg
    23401 ccttttcgag caaggtcctt ctccacgcgt tttcggatca gaggaagcgc gcgcccgaat
    23461 gcgatcatcc gtccatattt gtttccgtct cggacttcac gccatcgtgg atggtaccga
    23521 tattgtttgc ggccccgcgc atcccggccc gtggcctgga cgtgcccgtc ggcgtctaga
    23581 caaatccaca catcccgcca ggcgggaggt acggcaaggg aacggatcct ggtcaacgtt
    23641 tccccgtcgc ggaccggccg gccatccggc cacagatacc gcataccatg tcgctgccgg
    23701 accctcgcaa tgcccggtcg gcgatcatcc acgtaatgca aaccggcgga acgggctaca
    23761 gattttccgt cggcaacggc gtgccctccg acatgtggac tctgaacgga ctttcgcccg
    23821 atggtgcgct ccctctcgct catatctcaa aaactgggat tcgccatggc cagaccccct
    23881 tttgcatggc gaaacggatc atttatatat agccgctcac cgacgtttcg gtcttcaatg
    23941 gcttatccat catattatta agccgctcga cggccggacc tccgactgcg ggctttggct
    24001 ggtgccgccg gcctgtgagc tttccctttg gactcgacac tcttcttcaa tagagccatg
    24061 agatcgatga cttcgccttt ggccggccgc ttcttttcct cgggtgacgg ttccgtcacg
    24121 acttcggttt gaccggcctt aatgcgcttc tcgatcaatg ccaagaggtc ttcatgatac
    24181 gtgtccttgt attcctgagg tttccaagcg gcgctcatgc catcgaccaa ccggcgagcc
    24241 atttctattt cacgaggatt gacaccgact gatttgagat tactcgaagg cagatcaagc
    24301 tcagccgtat cccgcaactc attactgtag cgaagcgtgt tcatgacaat cacgtcaccc
    24361 accgggatga gagcggccac gtactgcctc gtgcgaatga cgacgttcgc aataccggtc
    24421 ttcccggttt gcttcagtgt ttcgcgtaac agcgcatagc ctttgtgtcc gcgtttgtcc
    24481 ggcgccaggt aataaggcgt ttcaaaatag atcggttcaa tctgttcctg ttcgacgaaa
    24541 gtcaggatgt cgacagtctg tgtggcctcc acgttcgcgc gtctgaaatc ttcatcggtg
    24601 agcaccacgt accggtcctt ttcatattcg tatcccttga cgatgtcgtc ccatgtcacc
    24661 tctttgccgt tctctttgtt gtaccgcttg aatccgaccg gcttcatatt gcgacgatca
    24721 agcatcgtga ggtcaaaact gttgcgcttc tcggcggagt acaatcccac cgggatgtga
    24781 accaatccga aactgatcgc acctttccag acgacgcgcg gcataagcac tcccctatga
    24841 tctgcgatcc cgagcgctga gtggtccatt gacgtctcgg gacggtgaac ttcaatgcat
    24901 caggctgcct gcctcacctc gcctttgtgc cctgcccctt ccctcgacgc ctgtgcaatc
    24961 tccgcgttaa tttcgccgcc aagcagcagc accataccgc tccaatagaa ccagagcatc
    25021 aggacgatga cccctgcgat cgacccgtat accttgttgt aatcgccaaa attgtcgacg
    25081 taggctttaa aaccgaggga gacgacgagc cacatactga cggcgaacac ggaaccaggc
    25141 gtgagccaat gccagcgttt ctcccgggtg tccgggcaga tgtgataaac cgcggccacg
    25201 accacgagca tgagggcgac gatgatcggc cactgcaaga cattccacaa ccaggtgaag
    25261 gccacaccca gcccgaacag gttcgcaatc cagcgcccga tcgtcggccc gtacagaacg
    25321 agtgccaacg agagaatgac gaagccggca agaccgatgg tgagcaccat ggcagtcagg
    25381 cggacacgcc aaaatggacg acggtcctcc ttcacaccaa acacgacgtt gagtgcatcc
    25441 atgattgccg tgacaccgct ggacgatgcc cacaaagctc ccaatgcgcc gagggagaga
    25501 atgttggccc cgctgccttc tgcgacctgg gtaaggaatc gctcgaccat ggagagcgca
    25561 tcctcgggca gcacgttatg caaatagctc atcagttcag gcatgaagct ctggactgaa
    25621 aagaggccca tcagagcggt caagaaaatt aaggccggga acagggcaag cagggcgtaa
    25681 taagagagtt gggcggcgcg accaagcagc tcgtgctctt ggcttttgcg ccatagccgc
    25741 cgtcccagtt cggcgagagt catgccggcc agcgtccatg gatgggacac cgtgcgacgg
    25801 atagcgggcg acgtgccggc ggtgcgactc attatgcgac tcgttcgaag ccggggagta
    25861 tcgctttgaa gaaggcgttt tgcaccaact tcgcgacgac ctgccacgta ttggcctgtg
    25921 gattttcaac cggtcccgac agattggtaa cggttgctgc ttcatcacga ggtttatttt
    25981 cgaagatgcc gagcacgccg ccggcgaccg cttcccacac ctttttcgac aaggctttat
    26041 ccttatcctg ttcggcatca tagacttcaa catctttgag catcggcttt acatacccgc
    26101 ggatctgatt atctttcacc gtcatctcgc tgaagaatgc aaacgtgcct tggtggatat
    26161 ccatgtcccc atgggcacgc aggacgtcat taaaggcgct gacattggtt ttgacgattt
    26221 tggcgttgag actgaagtcc ggccggggct tctccggacg gaatgtggca ttcatcacgg
    26281 tgggaccgct ccccataaat ttgcccgtga tcttgacgac tcctgttcct tcttcgagcc
    26341 gattactgag gttttgcagt tcgacctcca tgtcggtgag aaagacacga tagtccggcg
    26401 acgtcgcctt attgacaaac ccgatttcgc tgttgcgaat cttcccctgc tcgactttga
    26461 cccgcattgt tgaaccgtgc tgcgcttctt taacggtctc cgccgccttc ttcacccgtt
    26521 ggacttcttt atgcttcgtc gccgccgaat gcacgtagtc aatcttggca tcgtccaact
    26581 ggaactgatt gatggccacg acggaggacg tttccgaata ttgcacgtgg ccgcttacat
    26641 caagcactcc ctcacgacat tgcacgttga agcgtccggc caccggcagc aagttcttga
    26701 gttgcagccg ctcgattttc agatccgcat ccactttcgg caccgggatg gcgaaccaat
    26761 cggccctgcc ctcaagattg atctgtgatt ggtcgggaaa gttcgcgtgc acgcgaagct
    26821 tcgaaggata ttcttcgttc tctccaggcc tgttggttat gttggtcgct tcaatatcaa
    26881 acctttcgac acggagcgac tcagaagccg ccttgccgtc atcataagtc atgtgtccgt
    26941 tcgtcacgta gaacgcgccg cgaaatgcca tactctcccg taaacggtct tgccaggcct
    27001 ccgcctcttc cttcaccact tctttgtcgc ctttccgtaa aaatccgtct acatgctttt
    27061 gggtgagggc aaacaccggt tcctcaatcc gaagatcggc gccgatcttc cccagcaaca
    27121 aaggaccgag ttctgcatcg gcgctgatat taggaatcga gacgaccggg ggatctggac
    27181 ggatatcctg gcggacgacg atgtcacgaa ccgcaacgga caaggtgagc ggatggaggt
    27241 caaccgcacc gatggtgata tgaaagcccg gaagacgcga attggcttca gcctcaatgt
    27301 accgccgaag cggttcgtcg gccatggccg cgagaatcac cacggcgaga atcactatcg
    27361 cgacggcaat cagcgcaatc gtccacgcgc gacggtgctt cgacatgccc ttcggtggcg
    27421 gacttgtatc agataggacc ctggatcgga cataagaggt cctcttgccg ggtgccgacg
    27481 acaccgtatt actcgcagag cgtccggcgc tcgcctgaca cgtgctcggc cttcctctct
    27541 ggccgagtga gctggtcaag aagagattgg ccggcgatcc gaacgacgtc ttggagaaat
    27601 aaacggcctg cgtggatcgc ggtgttttgc aacctgctcc gctcctgcgc aagttcgtcc
    27661 gttagttcat tccacatgtc acgcaagggt cccaagagtt cacccatcgg tcggtcggac
    27721 cgtgaaccgg ccggcttgcg actgccctct gagcgtcggg gagcttcacg tccgtagaat
    27781 ggatacacgc cgcttggcag atcggactgg cctttttccg gcatcacatc cgcaccaccg
    27841 gcgcggggtg gataatacgg atacactcga gtcccccgcc tgtgctgttc cgccagaccg
    27901 gccaacaagc ctatcgcgat cgcggttccc accatgaccc atggccttcg gccggcttgc
    27961 agtgaagggt tcagttgttg cgtggtggat tcgatccaat cggcggccgt gtttttggcc
    28021 tggtcgagcg cgtccaacgc cgcggtcttg gtggtctgaa ccgtctcttg gactcgccgt
    28081 tcgagcgtgt caattttgtc ggccaacgcc aacctggtct ggaggatgtt cttcagatcc
    28141 tcttcaacgt catgcgctct ttgatccatt ggacattttc cttcatggta tggagactac
    28201 gacgaggcgt ggtatgaact gtgccagcca gcgaccgcgc ccggagaata agcgtcaaac
    28261 ccacgccggc cagcatgcca cccacaacac cgtaggagcc ccataatggc agccccgtcc
    28321 attcgtgcag gccatgcacc aacatgagca acagaaataa cgcaccaacc gcgccgagga
    28381 ccgcaccgat tccgaatccg gacgcagctc gtcccgcctt tgacaactcc agtttgacct
    28441 cgtcgcgcag cagttgggct tcctgccgca tcaggagttt ggcatcgtcg aggatgcccg
    28501 ttacgaggtt catcagacca aaaccggtcc ctcgcggtct gtcctgcatc atcacctcaa
    28561 tcg
//
LOCUS       DASSCQ010000157        21899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1635590, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000157 DASSCQ010000000
VERSION     DASSCQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 21899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21899
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635590"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..457
                     /gene="secG"
                     /locus_tag="VFH05_10945"
     CDS             104..457
                     /gene="secG"
                     /locus_tag="VFH05_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET6678780.1"
                     /translation="MHTLVIIIHIIACFLMIGAILLQSGKGAEIGAAFGGSSQTVFGS
                     RGPANFLSKFTVVVAAVFMLTSMGLAILAKEKTFSSTVIDLKKKDASQEVPATAPAQK
                     PDTDAHSSSDSPTAH"
     gene            complement(528..1439)
                     /locus_tag="VFH05_10950"
     CDS             complement(528..1439)
                     /locus_tag="VFH05_10950"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET6678781.1"
                     /translation="MDTQGKIWMDGSFVDWADANVHVLTHSLHYGLAAFEGLRCYKGK
                     SGSAIFKLQAHVDRLFESAHIGMMTIPFDKKQVAEAIVETVRVNRLEACYIRPLLYIG
                     YGAMGVYPGDNPVKLAIAAWKWGSYLGDDALANGMRACVSSFTRHHVNVSMTRGKISG
                     YYVNSILAKREAKANGYDEAILLDTEGYVSEGTGENIFIVRHGRIKTTPLTSILEGIT
                     RSAVIELAREQGIIVVEERFTRDEMYIADEVFVTGTAAELTPVREIDNRRIGTGKPGV
                     VTAALQKRFFAIVRGEDPSHDSWLTRV"
     gene            complement(1509..1880)
                     /locus_tag="VFH05_10955"
     CDS             complement(1509..1880)
                     /locus_tag="VFH05_10955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678782.1"
                     /translation="MKRAGTSTPRRGFVLRPYRRIPSWYGSYYLSGSVIGKGVVMNVS
                     CTGLRVLGDHSLKPGTDVSIRVTVDHGPPLEISRASVRWVKEYEFGLKIDQITSQTAN
                     RLVRLIHDSIDARPSPVRENA"
     gene            2025..2339
                     /gene="rplU"
                     /locus_tag="VFH05_10960"
     CDS             2025..2339
                     /gene="rplU"
                     /locus_tag="VFH05_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET6678783.1"
                     /translation="MYAIVETGGKQYRVENGTLVQVERLSGDVGGTVELSDVRLVHGD
                     NGLVVGQPLVKGAKVTAEIVDQGRTRSIMVFKKQRRKNYRRTKGHRQSFTKLRITGIH
                     TA"
     gene            2387..2650
                     /gene="rpmA"
                     /locus_tag="VFH05_10965"
     CDS             2387..2650
                     /gene="rpmA"
                     /locus_tag="VFH05_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET6678784.1"
                     /translation="MATNKGGGSSRNGRDSNPQYLGVKAYGGETVKAGAIIVRQRGTK
                     FFPGFNVGLGKDHTLFARVSGIVKFEGGRARQKVSVYPVSATS"
     gene            2774..3799
                     /gene="obgE"
                     /locus_tag="VFH05_10970"
     CDS             2774..3799
                     /gene="obgE"
                     /locus_tag="VFH05_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET6678785.1"
                     /translation="MFVDEVTITVKAGRGGDGSCSFRREMFVPRGGPDGGDGGDGGDV
                     ILIASRRLTTLLDLRYQRHYEAEDGKPGSGSNCTGRRGKSIQILLPVGTATYDQRTGE
                     VLADLVADGDSVTIAHGGKGGRGNSHFATSTNRVPTHFERGTAGEELELRLELKLLAD
                     VGLIGFPNAGKSTLIAAISAARPKIADYPFTTLVPNLGVVRYAEDRSFVVADIPGLIE
                     GAHEGKGLGIQFLRHIERTSLLLHLIDVSEWTAEDPVASFDIMRHELAAYNPSVAARP
                     FAVVATKIDAAGDGTRLSELQAFCQQGGIPCLPVSSATRAGLTELIGFVGQRLEQLRG
                     MACETKS"
     gene            3781..4902
                     /gene="proB"
                     /locus_tag="VFH05_10975"
     CDS             3781..4902
                     /gene="proB"
                     /locus_tag="VFH05_10975"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET6678786.1"
                     /translation="MRDQVLKQARRIVVKIGSSLVSSREAGLEPERIDQLADDIALLR
                     SAGREVLIVSSGAIVSGINKLALKEYPKSLPVKQAAAAVGQSRLMWAYEKSFERLGLK
                     VAQILLTHHDLADRRRFLNARHTLTALIEFQVIPIINENDTVAVDEIRVGDNDTLAAE
                     VAHLVDADLLVILSDIDGLFTEDPRKSPSAQLIPLVTDINEDIERRAGVSVTFGATGG
                     MATKVRAAKKVGEYGVATLIVNGQTRGLLPQILSGIPGGTLFLAKERRLTSRKHWIGF
                     TLRARGTVSVDQGAVEALTKRGKSLLASGIVQVTGSFDAGDAVSCLDPDGKEFAKGLV
                     NCSSDTLSKIKGLKTAEIQQRLGPQDYEEVIHRDNLVIF"
     gene            4971..5630
                     /gene="nadD"
                     /locus_tag="VFH05_10980"
     CDS             4971..5630
                     /gene="nadD"
                     /locus_tag="VFH05_10980"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET6678787.1"
                     /translation="MGVGLLGGTFNPVHNGHLDIARQTRDVLHLERIVFIPTGDPPHK
                     HQEDLASARDRYEMVQLAIASDPSFTVSDIEVRRPGKSYSVDTIRLLLQQHGGSVPLY
                     FLIGLDAFLELPTWRDPAIALTLCSFVVISRPGASFQALGTLPLIPRIPTQSLVDLDE
                     GCSSRLDVPIGEQVLICLRLPPCEVSASDIRTRVRQGLPVATLLPPAVESYILQHHIY
                     S"
     gene            5674..6063
                     /gene="rsfS"
                     /locus_tag="VFH05_10985"
     CDS             5674..6063
                     /gene="rsfS"
                     /locus_tag="VFH05_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET6678788.1"
                     /translation="MARAMLDKKATDVLILHVAKLTSVADYLVLGSAESDRQTRSIAD
                     HVDGILSQAGGGALSIEGKASSQWVLMDFGDVIVHVFRQDARHHYGLERLWADAKRVA
                     IPGYESPPPLLTSKSVTRTRARAAKRA"
     gene            6068..7462
                     /locus_tag="VFH05_10990"
     CDS             6068..7462
                     /locus_tag="VFH05_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678789.1"
                     /translation="MLRLLTTIFLISTGIFVYSYFRELNPGTITVHTGPSSQFELSAV
                     TLVVFSMAVGAVLVALAVGMRQTVHVIGNWRSTRLQRRKEKVDALHREGTHAFMSKRT
                     SDAVGLFEKALSIDPNRIDSLLWLGNIYRSETNYAEAIRLHQNAHRIDDRNIEILLEL
                     SKDLEGAKRYEEALQALQKILRIEPDNLTGLTRKRDLLIRLEKWSDALEIQHRMAKAN
                     LPEAERRAEGQLLTGCMYEVGRQLLERGHADKARRYFRGAIKKDRTFLPAYIGLGEVL
                     IREGKTKEAVEILKKVYAKTHNVIILHRLEELFLEQGEPSEIIRIYQEALQQDPHNAA
                     LQFYLGKLYYRLEMVDEAFDILSTVEGPQDQLADYHKVMANLFLRKQHMEQAVMELKK
                     ALQFKKRVVVPYVCTACQQDTTEWSGRCRRCGNWNTFLALPWEGGAGAGRDGAPAAGR
                     TVPYQGIASPFETV"
     gene            7548..8564
                     /gene="bioB"
                     /locus_tag="VFH05_10995"
     CDS             7548..8564
                     /gene="bioB"
                     /locus_tag="VFH05_10995"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET6678790.1"
                     /translation="MTDYAVFAQKALNDEVLSRQECRALLNAPDDDLLEVLQAAYLVR
                     SRYFGKTVRLQMLQNAKSGACQEDCHYCSQSAVSTAAIERYNLVPRAQMVAGARQAAA
                     SKAQRYCIVISGRSPLDREIDEIAGAVREIKQELPIQICCSLGLMNEGQARRLKAAGV
                     DRVNHNLNTSESFHSSICTTHTFQDRLATIRNARAAGLEICSGGIVGMGEHDDDLIDL
                     AMALRDVKPDSIPLNALHPVEGTPLEDCDHLTPARCLKILCLFRFLHPRTEIRIAGGR
                     EHNLRSLQPLALYPADSVFVNGYLTTPGAPAPEVWGMIRDLGFTIEVDYQPAPAADSL
                     NVQR"
     gene            8706..9890
                     /locus_tag="VFH05_11000"
     CDS             8706..9890
                     /locus_tag="VFH05_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6678791.1"
                     /translation="MATDERTTTCSPGVYPQVGQPKAQDPPLTTVNLPADSRSLAEVI
                     VAVVASIFALYWAQRFFIPLFLGIILAYTLNPLVTWMERIKIPRLVGTTMVMMTLLFG
                     LALGTLSLRGQVERILDQVPDAAGKLSVALKDMAAQPNTVLKVQAAAREIEQATTQAA
                     SGTTASQKPPTRVVIEEPKFKLLTWLWTGSLGMFGIIGETAMMLILVIFLLLSGDTFK
                     RKLMRISGPSLSKRKITAAILDDIDRSIQNYMLTLLMANVLLGVLTWLAFRLIGLDNA
                     GAWAVAAGVLHFIPYLGPALTAVATGMAGFMQFESLSMAMLVAASSLIIATLVGMVLV
                     TWMCGKISNMNTTAVFVALLFWGWLWGIWGLLFAIPITGMVKVFAERVEDLRPVAELL
                     SD"
     gene            9883..10758
                     /locus_tag="VFH05_11005"
     CDS             9883..10758
                     /locus_tag="VFH05_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678792.1"
                     /translation="MIDPPVTSHARITAMIRGCFPYLCGLVIVAAFFVTLCETEARPI
                     AVRFLEGLSHGFLLVRSSAGEIIGEGELTQVMKESGVTESRLVFRFKDGSLHDERVAF
                     SQQRVFTLIRYQLVQRGPSFPEQIDVTVDRATSKYEVRSRVRENDNEKVLAGEIDLPK
                     DAYNGMIVTTLLNLPKASSESVQVLAFTPKPMAIPLELSFVGEQVIRVGEQSRKALQY
                     TFKPDIGPIKKFLGKVLGKLPADFHYDCLILADDVPSFVRFQGPLQLMGPILTIELTS
                     PQAAETPGDQKHVSK"
     gene            10773..11018
                     /locus_tag="VFH05_11010"
     CDS             10773..11018
                     /locus_tag="VFH05_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678793.1"
                     /translation="MNPIFSLLIKQLPRLLPVVTSMLERPPSRNTEDGRLAGIEQSIE
                     WLAERSDAIERRLKRLTMLVVAGLLLSVVAFVLMLVR"
     gene            complement(11024..11725)
                     /locus_tag="VFH05_11015"
     CDS             complement(11024..11725)
                     /locus_tag="VFH05_11015"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HET6678794.1"
                     /translation="MTASNDSLTTASKSLIPQVMLAWYAVLFLFLAISPFDPRIWWGS
                     NILPVAFVAVLVLTHRTFPLSNTSYALITLWLTLHTVAVHYTYPKVPVGDWLDRWFDF
                     HRNHFDRIVHFSFGFLMTLPLVEVFRRRCNAKGWLLPYLVVMTVLGFSAFWEIVEAWI
                     GQLAHPDIEKAMVGHQGDVWDPQRDMASALYGSLVYVGYLAWSRALHEERATPDCVSD
                     SDGLLNSRMDAGQGI"
     gene            11910..13088
                     /locus_tag="VFH05_11020"
     CDS             11910..13088
                     /locus_tag="VFH05_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET6678795.1"
                     /translation="MRIDDPAFRTSLEAFAGAPILGDNRVDILLNGEETFPALLDAIR
                     SAHHTITFEAYIFHEGKIADQLVGAFVERCKAGVRVAILLDAHGSDGLPERYIQDLRD
                     AGCILVPDFRPLSLWSLERTNKRNHRRIMVIDGLVGFTGGYGVDDTWSGNGRTEGRWR
                     ETNVRLQGPIVQSLQEAFVEHWREATSVLLGGKDYFPYPPVTVKDVPVLAQVVRSSPL
                     QGNDAMYRVFLQAISSARTSILISTPYLLPGEQLTEALLDAVRRGVRVRVLVPSVVKS
                     SGVEFVTQASQREGFGALLEGGVQLHEYSPALLHTKMMIIDGTWATVGSTNLDNRSMA
                     MNDELNVMFYDRTIAKRLEEIFAADVAYSHKVSREQLENQEWLHRALGVLLSPFHAWF
                     "
     gene            complement(13096..15726)
                     /locus_tag="VFH05_11025"
     CDS             complement(13096..15726)
                     /locus_tag="VFH05_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET6678796.1"
                     /translation="MPKSTMTAWLRLVGMLIGAHGWQRPFRTALTVAGVALGVLSSVA
                     ILTANREVLRAFERAVLTVAGPASLEVSGRDLGLDETVLEVIRHAPGVQRVIPLIEEA
                     VVLGEGAPAGESVQVYGMDLVAEAESLGFRVERADGDDLLNDLLEPDRVFLGRQLSDE
                     LNLVPGSTFKARAGSRWMPLKVAGLVQGSDRPKSYWNRVAIMDLAAAQVLFQMVGRLD
                     RVDVVTAPNRTPEEVAVALRAVLSPSVVVQRPAQRTAQVEKMVGSFQLNLAVLSWVGL
                     LVGIFLVYNTMSFSVAQRRREIGIYRALGMTERRVAGLFLLEAGLLGCLGGLLGGVAG
                     LWMARSMVVLVSRTISDLYVPLGVTGAFSIVDGQSAEAVAQGLVVGIVVSMAGAVAPS
                     IDASRTVTTKALAPGDYETSVAMRSQRYLLGSLALLIAGALCTLGPPFKGLPIFGYAA
                     TLCLLAGFALLAPVCILALRWSTQHPKEAPSLGTNLRRMAADHAARHPGRNAVTVSAL
                     LVGLAIMIGVVIMVRSFRETVEAWVNETVMADLIIAPPAWLHSKQSGQASRSLPAGWL
                     PLLQALNGVAAVDAYRDVEAEVEGEPVALISRDLRLHAEHGRYLMVEGEGASVLRRAA
                     DTGGVLVSEVLVNRLGLQPGSLVTITTPKGPHTVPIEGVFYDYATDGGKMVMERSVYR
                     RFWDDDRVTVFGIYVSPDADEERVARSITEGLAGHELSVAPPIIIRNKDLRREILTIF
                     DRTFVLTYVLEAIAVFVAVLGIVNTLVTAVMERRRELATLLAIGASRAQVERLVLWEA
                     IYLGLIGALLGVMAGLALAWVLIAVVNKQSFGWTIRMTIPLEVLIQAVLLAIGAAGVA
                     GYLPARWAARQPLVEGLRDE"
     gene            15885..16151
                     /locus_tag="VFH05_11030"
     CDS             15885..16151
                     /locus_tag="VFH05_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6678797.1"
                     /translation="MKTALRRKVDTLIEESVDLELLTPRQRDILRLVSIGHTNREIAN
                     MLQISVRTVEVHRFNLMRRLNVRNVAQLLRRALQLGLLSKSFAK"
     gene            complement(16226..17152)
                     /locus_tag="VFH05_11035"
     CDS             complement(16226..17152)
                     /locus_tag="VFH05_11035"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET6678798.1"
                     /translation="MDLTVDIAGVKFPSCFMNASGALCVTEDELMALGGSDSGAIVTK
                     SMTIEARQGNPMPRYHGFPGGSINSMGLPNLGYKAYAALIPALKQFGKPVIASVAGLC
                     EEDFPTIAQVINHAKPDLIEVNLSCPNIPGKPQIGYDADTSERIVKRLRKIVTVPMGV
                     KLPPYFDPAHHKLMGDVLARCGVDFLTLINSVGNGLVVNPDAETVVIKPKGGFGGLGG
                     AMIKPVALANVRAFWKHFHGTMPIVGTGGVVDGVDAFEHVLCGASAVQIGTVLVEEGV
                     QVFARLEHELTACLEKKGYKTIGDCRGKLKEL"
     gene            complement(17187..17903)
                     /locus_tag="VFH05_11040"
     CDS             complement(17187..17903)
                     /locus_tag="VFH05_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6678799.1"
                     /translation="MLPPLIAPIVKLTEVSKLYERGDDRICGLDRVTLEVTAGAFCAF
                     VGPSGCGKSTLLNLIAGLDLPTSGDIQLNGQSTAHYNADDWTRVRRQCIGIVFQAFHL
                     VPGLTVEENVAFPLLLRGDERRRVAERVSEVLALVRMNHRARHRPSELSGGEQQRVAV
                     ARAIVHQPRIVLADEPTGNLDSQHGGEIISLLRTLARDFQLTVLLVTHSAAAAEAADY
                     IWAMKDGRLLTRTERSAASC"
     gene            18002..18796
                     /locus_tag="VFH05_11045"
     CDS             18002..18796
                     /locus_tag="VFH05_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET6678800.1"
                     /translation="MLRWCRLALRIVLPVDCISCDQPLRTDQAPRFCDACWQRIHPLS
                     GPRCSHCDQPFVAAAATSWTPGHTCQHCREHPPAYHRAWTLYPYIPPLQDAICALKYR
                     GVSALAGPLAGLMIRALPPDIECDVIIPVPLHPSRLRVREFNQSLLLADRLGRFFMRP
                     VSMTSLIRTVATEPQTALTRRQRLRNLRRAFAVRDAEPLVGRRILLVDDVYTTGTTLN
                     ECAKTLLEAGALSVSALTLARTLDSSLVPDRIWADHADRSLTGFGL"
     gene            18801..18935
                     /locus_tag="VFH05_11050"
                     /pseudo
     CDS             18801..18935
                     /locus_tag="VFH05_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
     gene            18963..19142
                     /locus_tag="VFH05_11055"
     CDS             18963..19142
                     /locus_tag="VFH05_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678801.1"
                     /translation="MESLADDSALAGLDENDPGSMERLMKRMGNEMGAEAGDDLEAMD
                     NSDEGVGDGDGLDSL"
     gene            19248..19445
                     /locus_tag="VFH05_11060"
     CDS             19248..19445
                     /locus_tag="VFH05_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6678802.1"
                     /translation="MTKAPKSELMTAEETCRYLKITSRTLYRYLHNHQIPAFKLGKEW
                     RFVRSDLEQWIRDRTRGPVPS"
     gene            19462..19902
                     /gene="mraZ"
                     /locus_tag="VFH05_11065"
     CDS             19462..19902
                     /gene="mraZ"
                     /locus_tag="VFH05_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET6678803.1"
                     /translation="MFAGEYLCKVDEKGRFIVPSPIREQVDSEGQAVVFLKGPEESIL
                     IYSMKEWEKVLERTKTTLDEEQSRLFMHFVVSEAGTSELDKAGRILIPGKLRKLVPLD
                     DDQEIILVGLYHRLEIWNPSEWRRYIARSEGRYEQNMAKILNLL"
     gene            19965..20510
                     /locus_tag="VFH05_11070"
     CDS             19965..20510
                     /locus_tag="VFH05_11070"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HET6678804.1"
                     /translation="MPGYRRPIRIPASSLPPTLVSFRTSRSIRRPAQPQAAILTHLNA
                     AALSALHEEQRSITLTLPVPPSINHQYATVNGRRLLSSTGRSYKAQVGQLVWIRLAAS
                     PHKTVLLSQLQSSALALSIRFYFTSALRRDVDGGLKIAQDAICEGLGLNDNRIIETHL
                     YKHVDRRNPRIEVVLSATSLA"
     gene            complement(20519..21754)
                     /locus_tag="VFH05_11075"
     CDS             complement(20519..21754)
                     /locus_tag="VFH05_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6678805.1"
                     /translation="MPAFIWLTVMTALRILARNRLRAGLTMLGIVIGVGAVIAMVSIG
                     EGAKRAVQAQIATMGTNVIIVWPGATTVSGVRGGQGGAVTLTVSDALDLKKKIPLLSE
                     TGWARREVMQMVNGNRNWNGPINGVSPSYLTIRDWAFSSGGPFTQADLDSAARVALIG
                     QTIVDNLFDEGEEPVGAVIRIRNVPFRVIGVLSPKGQSAQGSDQDDVIFIPFTTAERK
                     VFGTMFLGSVAALFASTDRPEDLPEAVERIREVLRGRHRLQAEQGDDFTVKTQVDIGK
                     VQEGTNYTLTLLLFSVASVSLLVGGIGIMNILLVSVTERTREIGVRMAVGAKRRHIVM
                     QFLIEAMTLSLLGGAVGVVLGVVGAKLTTVVAGWPTVISTDVILIAFFFSLAVGLFFG
                     LYPANKASRLNPIEALRYE"
BASE COUNT         4701 a         6552 c         6096 g         4550 t
ORIGIN      
        1 cggagatcga tggcgcgctc gtcggcgggg cttgtctcaa aacagagtca tttgctacaa
       61 ttgcgctcgc tgctttgaac cgacggagtg ggtgacgggg tctatgcata ctttggtgat
      121 catcattcat atcattgcat gcttccttat gatcggagcg attctcctcc agtcgggaaa
      181 aggagcggaa atcggcgcgg ccttcggcgg atcgagccag acggtattcg gtagccgcgg
      241 ccccgcgaat tttctcagta agttcacggt cgtggtcgca gcggtcttca tgttgacctc
      301 catgggactt gcgatacttg cgaaggaaaa gacgttctcg tccaccgtca tcgatcttaa
      361 gaagaaagac gcttctcaag aagtgcccgc cactgcaccc gcccaaaaac cggacacgga
      421 cgcacactcc tcgagcgatt cccctactgc ccactgatcg acacgaagcc cggacaattc
      481 ggtgtggcta gacttgggcg ggattgatga agtttctcag cgtctcgcta gactcgggtg
      541 agccaggaat catgcgaggg atcttctccc cgcacgatcg cgaaaaaccg tttttgcaac
      601 gcggccgtca cgacgcccgg ctttccggtc ccgatccggc gattgtcgat ttctctgact
      661 ggagtcagct cggcagcagt ccccgtcaca aatacttcgt cggcaatata catttcatcg
      721 cgcgtgaacc gttcttcgac cacgatgatg ccctgctccc gtgccagttc gatgacggca
      781 cttcgtgtaa tgccttcgag gatggacgtc aggggggtcg tcttgatccg gccgtgccgc
      841 acgatgaaga tattctcgcc tgtcccctca gacacgtacc cttccgtatc caggagaatg
      901 gcctcatcat agccgttagc cttggcttcc cgtttggcta agatcgagtt gacgtagtag
      961 ccggaaatct ttcctctcgt catcgacacg ttgacgtggt gtctcgtaaa tgaggagacg
     1021 caggcccgca tgccgttagc caaggcgtca tcgcccaggt acgaacccca cttccacgcg
     1081 gcaattgcca atttgaccgg attatcgccg ggataaacac ccattgcgcc atacccgatg
     1141 tacagaagcg gacggatgta acaggcctcg aggcgattga ctcggaccgt ctcgacgatg
     1201 gcctcggcca cctgtttctt gtcgaaggga atggtcatca tgccgatatg ggccgactca
     1261 aacaatcgat cgacatgtgc ctgcagcttg aaaatggccg aaccggactt tcccttgtag
     1321 caacggaggc cttcgaaggc ggccagtcca taatgcaaag agtgagtcaa gacgtgcacg
     1381 tttgcgtccg cccaatcgac gaacgaaccg tccatccaga tcttgccttg cgtgtccata
     1441 ctcgagcgga ctatagcagc ggttttgaag aagcgtcaag caaacgagga agtcaatcgc
     1501 gcggtacgct atgcgttttc ccgcacaggg gaaggacgag cgtcgatgga atcgtgtatg
     1561 agtctgacaa gacgattcgc agtctgagac gtgatctgat cgatcttcaa cccgaactca
     1621 tactccttga cccagcggac cgaggctcga gaaatttcca gaggaggacc atggtccaca
     1681 gtcacccgaa tggacacgtc ggttcccggc ttgagagaat gatctccgag cacacgcagt
     1741 ccagtgcagc tcacgttcat gaccacgcct ttgccgatca cgcttccgct caggtagtag
     1801 gaaccatacc agctagggat gcgccggtac ggacggagca cgaatcccct ccgcggcgtc
     1861 gatgttccgg ctctcttcat ggcttcactc tacgccgccc ctactgccaa gaacagccta
     1921 cgaacagggg ggctcctgcg actggctgcc agtgcgtccg cttccttgac actcttgaaa
     1981 tcactgtgat acataacgac gttctgttag ggaggcaagg gctcatgtat gcaatagtcg
     2041 aaacaggcgg caagcagtat cgggtcgaga atggaaccct cgttcaagtg gaacgtcttt
     2101 ccggcgatgt gggtggcacc gtggaactga gcgatgtgcg gctcgtccat ggcgacaatg
     2161 gcctggtcgt cgggcagcca ttggtcaagg gcgccaaggt caccgctgaa atcgttgacc
     2221 agggccggac tcgttcgatc atggttttta agaaacagcg ccgcaagaat tatcgccgca
     2281 ccaagggaca tcgtcaaagt ttcaccaaac ttcggatcac aggcatccat acggcatagt
     2341 agtcggctca tgaagagcga tatcgcaccc gacgcaagga tctgtcatgg caacgaataa
     2401 aggcggcggt tcgtcaagaa acgggcggga cagcaaccct caatatctcg gggtgaaagc
     2461 ctacggcggt gagaccgtga aagcaggcgc catcatcgtg cgtcaacggg ggacgaagtt
     2521 cttccctggt tttaatgtcg gcctcggcaa agaccacacc ctcttcgccc gcgtgtcggg
     2581 aatcgtgaaa ttcgagggcg gacgcgcgcg ccagaaggtc agcgtctacc ccgtgtccgc
     2641 gacctcttaa catcgcacac tccccttgag tcatcacgca gtccgttccg gctgacggcg
     2701 agcttgcgtg cagcggtgcc cgcgcggtcg gcatcgtcac gattgcttcg tcaccgtcca
     2761 cgagtaggac ttcatgttcg tcgacgaggt caccatcaca gtaaaggcgg gtcgcggcgg
     2821 agacggcagc tgtagtttcc gacgagaaat gttcgtgccc cgcggcggtc cggacggcgg
     2881 cgatgggggt gatggcggtg acgtgatcct gatcgcttcc cgacgcctga cgacgctcct
     2941 cgaccttcgc tatcaacggc attacgaggc agaagacggg aagccgggca gcggatcaaa
     3001 ttgcacgggc cgaaggggca agtcgattca gatcctgctg cccgtcggca ccgcgacgta
     3061 cgaccaaagg accggcgagg tcttggctga cttggtggcg gatggggact cggtgacgat
     3121 tgcacacggg gggaaaggtg ggcgaggaaa cagccatttt gcaacgtcaa ccaaccgcgt
     3181 ccccacccac tttgagcgcg gtacagccgg cgaggaactc gaactgcggc tggagttgaa
     3241 gctgctggcc gacgtcggcc tcatcgggtt tcccaatgcg ggaaaatcca cgttgatcgc
     3301 cgcaatctcg gcggcccgcc cgaaaattgc ggattatccc ttcaccaccc ttgttccgaa
     3361 tctgggtgtc gtgcggtatg ccgaagaccg aagttttgtg gtcgccgata tcccgggact
     3421 gatcgaaggc gcgcatgaag gaaaaggcct tggcattcaa ttcctacgac acattgagcg
     3481 cacgtcactg cttcttcact tgatcgacgt atcggagtgg acggcggaag atcctgtggc
     3541 gagctttgac atcatgcgcc atgaactggc ggcatacaat ccgtcggtcg ccgcgcgtcc
     3601 ctttgccgtg gtcgccacga agatagatgc ggctggcgat gggaccagac tcagcgagct
     3661 tcaagcattc tgtcagcaag ggggcattcc gtgtcttcct gtttcatcag cgaccagagc
     3721 cggactgacg gagttgatcg gtttcgtcgg tcaacgcctc gaacaactgc gcgggatggc
     3781 atgcgagacc aagtcctaaa acaagccaga cgaatcgtcg tcaaaatcgg gagcagtctg
     3841 gtgagctctc gcgaggccgg tctcgaaccg gagcgcatcg atcaactggc ggacgacatc
     3901 gctctgctcc gctcggcagg tcgtgaagtg ctgatcgtgt cctccggcgc catcgtttcc
     3961 ggcatcaaca agctcgcgct caaagaatat cccaagagcc ttcctgtcaa gcaggcggcg
     4021 gcagcagtgg gacaaagtcg gttgatgtgg gcgtacgaga aatcctttga gcgccttgga
     4081 ctaaaagtgg cgcaaatact tctgacgcat cacgatctcg cagaccggcg ccggtttttg
     4141 aatgcccggc atacgctgac cgctctcatc gagttccagg tgattccgat catcaatgaa
     4201 aacgacaccg ttgccgtaga cgaaattcgt gtcggtgaca acgatacact ggctgccgaa
     4261 gtggcccatc tcgtggatgc tgatctgctc gtcatcctgt cggacatcga cgggttattt
     4321 acggaggatc cgcgcaagag tccctccgcc cagttgatcc cactcgtcac ggacatcaat
     4381 gaagacatcg aacgacgggc cggcgtatca gtgacgtttg gtgccaccgg tggcatggca
     4441 accaaggttc gtgccgctaa gaaagtgggt gagtacggag tggcaaccct gattgtgaac
     4501 gggcagactc gtggcctgct tccccaaatc ctgtcgggca ttccaggcgg caccttgttt
     4561 ctcgcgaagg aacgccgact gaccagccgt aagcactgga tcggtttcac gttgcgggca
     4621 cgagggacgg tcagcgtcga tcagggagcg gtcgaagccc tgacgaagcg ggggaagagt
     4681 ctgctggctt ctggcatcgt ccaggtcacc ggttcgttcg acgccggcga tgcagtcagc
     4741 tgtctggacc cggacggcaa agaattcgcg aaaggtctcg tgaattgttc ttccgacacc
     4801 ctttccaaaa tcaaaggcct gaaaacggcc gagattcagc aacgacttgg ccctcaggac
     4861 tacgaggaag tcatccatcg cgacaatctc gtcattttct gagccgtgag tgttgggtga
     4921 tgcaacccgc cggcgaaaac tcagcacccg atgtacagac ttcggaaccg atgggcgtcg
     4981 ggctcctggg ggggactttc aaccctgttc acaacggcca tctcgatatt gcacggcaga
     5041 cacgggatgt ccttcatctc gaacggatcg ttttcattcc gaccggcgac cctccgcaca
     5101 aacatcagga agatctcgcc tcggccagag accgttatga gatggtccaa ctggctatcg
     5161 cttccgatcc ttccttcact gtctccgaca tcgaggtgcg ccgacctgga aaatcctatt
     5221 ccgtcgacac catccggctt cttctgcagc agcatggggg gagcgtcccc ctgtatttct
     5281 tgatcggatt ggacgccttc ctcgagttgc cgacctggcg tgacccggca atcgctctca
     5341 cgctctgttc gttcgtcgtc atttcgaggc ccggtgcctc ctttcaggcg ctcggcacct
     5401 tgcccctcat tccccgcatc cccacgcaat cactggtaga cctggatgag ggatgcagtt
     5461 caagactcga cgtccccatc ggcgaacaag tgcttatctg ccttcgattg ccgccgtgcg
     5521 aggtatcggc ttccgatatc cgcaccagag tcaggcaggg gcttcccgtg gcaactctat
     5581 tgccgcctgc cgtggaatcc tatatacttc aacatcatat ctactcataa gaggacgccg
     5641 atcgcacgaa catcgaaggc cactgcccta gccattgcga gggccatgct ggacaaaaag
     5701 gccacggatg tcctcatcct ccacgttgct aaactgacct ccgtcgccga ttacctggtg
     5761 ttgggttccg cagagtccga ccggcaaact cgatcgattg ccgatcatgt tgacggcatt
     5821 ctatctcaag ccggaggtgg tgcgctgagc atcgaaggga aagcttcgtc tcagtgggtg
     5881 ctgatggatt tcggcgacgt gatcgtccat gttttccggc aggatgcccg ccatcactac
     5941 ggattggagc gcttgtgggc ggatgccaaa cgtgtcgcta ttcctggcta cgagtcgccg
     6001 ccgccgctgc tcacctcgaa atcggtgacg cgcacgagag cgcgtgccgc aaagcgagca
     6061 tagtgcaatg ctcaggctgc ttaccacgat ttttctgatc agtactggca tcttcgttta
     6121 cagctacttc cgcgagctca atcctggcac gatcaccgtt cacaccggac catcgagtca
     6181 atttgaattg agcgccgtga cgctggtggt cttttcgatg gcggtcggcg cggtattggt
     6241 ggccctcgcc gtcggcatgc gacagaccgt gcacgtgatc ggcaactggc gaagtactcg
     6301 gctccagcgg cgcaaggaga aggtcgacgc cctgcatcgt gaagggactc atgccttcat
     6361 gtccaaacgc accagcgacg ccgtggggct ctttgaaaaa gcgctttcga tcgatccgaa
     6421 tcggatcgac tcattgctat ggctgggaaa catctaccgc tctgaaacca attatgccga
     6481 ggccattcgc ctgcatcaga atgcacaccg catcgacgac cggaatatcg agattcttct
     6541 cgagttgtcg aaagacctcg aaggggcaaa gcgatatgag gaggcgctcc aggcgctgca
     6601 aaagatcctc agaatcgaac cggataacct gacgggactc acgcggaagc gggatctcct
     6661 gattcgtttg gagaaatgga gcgacgcgct cgaaattcaa caccgcatgg ccaaggctaa
     6721 cctccctgaa gcggaacggc gagcggaagg ccagctgctg accgggtgca tgtatgaagt
     6781 gggccgccag ttgctggaac gcggccatgc tgataaggcc aggcgatact tccgcggcgc
     6841 aatcaagaag gatcgcactt tccttccagc ttacatcggc ttgggcgagg tgctgatccg
     6901 tgaaggcaag acgaaagagg cggtcgaaat cctgaaaaag gtctacgcga agacccacaa
     6961 cgtcatcatt ttgcaccgcc tggaagagtt atttctggaa cagggcgaac ccagcgagat
     7021 catccgcata tatcaggagg cgctgcagca ggatcctcat aacgccgcgc tccagttcta
     7081 tctcggaaag ctctactacc ggctggaaat ggtcgacgaa gccttcgata tcctatcgac
     7141 cgtcgaaggc ccccaggatc aactggcgga ttaccacaag gttatggcca atctgtttct
     7201 tcgcaagcag cacatggaac aggcggtcat ggaattgaag aaggcgctgc agtttaaaaa
     7261 acgcgttgtc gttccctacg tctgcacggc atgccagcag gacaccactg agtggtcggg
     7321 acggtgccgt cggtgcggca actggaacac cttcctcgcg cttccgtggg aaggaggcgc
     7381 gggagccggc cgcgatggcg cacctgcggc gggacgcacc gtcccctacc aaggcattgc
     7441 ttctccattc gaaacagtgt agttttttcg cgcggctacg cagggcgaga ttgttgaatg
     7501 cgtcttcgcg ctccacgtcg atgatgatcg ccgtgaaagg gactgagatg acggactatg
     7561 cagtgttcgc tcaaaaggcg ctcaacgacg aggtcctttc gcgtcaggag tgtcgtgccc
     7621 tgctcaatgc tcctgacgac gacctgctcg aggtgctgca agccgcttac ctggtgcgct
     7681 cccgctattt cggtaagacg gtgcgcctgc aaatgctgca aaatgcgaag agtggcgcgt
     7741 gccaggagga ttgccactat tgctcgcaat cggcggtgtc aacggcggcg atcgaacgat
     7801 acaatttagt gccgcgcgca cagatggttg cgggtgcgcg gcaggccgcc gcgtcgaagg
     7861 cgcagcggta ctgtattgtc atcagcgggc gcagcccgtt ggatcgcgag atcgatgaga
     7921 tcgccggcgc cgtccgagag atcaaacagg agctgcccat ccaaatctgc tgctcgctcg
     7981 gcttgatgaa cgaaggccaa gccagacggt tgaaggccgc gggagtagat cgggtcaacc
     8041 acaacttaaa cacgagtgag tcctttcaca gctccatctg caccacgcac accttccagg
     8101 atcgtctcgc cacgatacga aacgcacggg cagccggcct cgaaatatgc tcgggcggca
     8161 ttgtggggat gggagagcac gatgacgacc tcatcgatct cgctatggcg ctacgcgacg
     8221 tgaaaccgga ttccattccg ttgaatgccc tgcatcccgt ggaagggacg ccgctggagg
     8281 attgcgacca tctcactcca gcacgctgtc tcaaaatact ctgtctcttc cgtttccttc
     8341 atccccgcac agaaattcgc attgccggcg gtcgagaaca taacttgcgc agcttgcaac
     8401 ccttggccct ctatccggcg gattctgtct ttgtaaacgg gtacttgacc accccaggcg
     8461 cgccagcgcc agaggtgtgg ggcatgattc gtgatctcgg tttcaccatc gaagtggact
     8521 accagccggc tcctgctgcg gattcgctca acgtgcagcg ctaagtttcc cccatatcgc
     8581 ggtgttccct ccatctgaca cgaaccattc caccgtcgac caggacttct cctagtaggg
     8641 atttcccctg gtgcgtggtg tcatggctgt agagcggcct tgacttcccc gggcactgat
     8701 gcggcatggc gacagacgaa cgtacaacaa cgtgctctcc cggcgtctac ccacaggtcg
     8761 ggcagccgaa ggcgcaagac cctccactga caacggtgaa tctgccggcg gattcacgaa
     8821 gcctggcaga ggtcattgtc gcggtcgttg cgagcatttt tgcactctat tgggctcaac
     8881 ggttttttat tccgctcttc ctcggcatca tcctcgccta cacattgaac cctctcgtca
     8941 cgtggatgga acggatcaaa atcccgcgtc tcgtcgggac gactatggtg atgatgacac
     9001 tgctgttcgg tctagcgctg gggactttgt cgctccgcgg acaagtggaa agaattttgg
     9061 atcaggtgcc ggatgcggcc gggaaattgt cggttgcgct caaggatatg gccgctcagc
     9121 ctaatacggt actgaaggtc caagcggcgg ctcgagagat cgagcaggcg accacccagg
     9181 cggccagtgg aacgaccgcc tcacagaaac ctccgacccg tgtggtgatt gaagaaccaa
     9241 aattcaaact ccttacctgg ttatggacgg gatctctcgg aatgttcggc atcatcggcg
     9301 aaacagcgat gatgctgatc ctcgtgatat ttctcttgtt atccggtgac acattcaagc
     9361 ggaaactcat gcgcatcagc ggcccttcgt tatcgaagag aaagatcacc gccgcgatcc
     9421 tcgacgacat cgatcgatcc atccagaatt acatgctcac gcttctgatg gccaacgtcc
     9481 tgctcggagt cctcacctgg ctcgcgttcc gcttgatcgg gcttgacaac gccggcgcat
     9541 gggcggtcgc cgccggcgtg ttgcatttta ttccttatct ggggccggcg ctgaccgccg
     9601 tagccactgg gatggccggg ttcatgcaat tcgagtcatt gtccatggcc atgctcgtgg
     9661 cggccagctc tctcatcatt gccacgctgg tgggcatggt tctcgtcaca tggatgtgcg
     9721 gcaagatttc aaacatgaac acgactgccg tgtttgtcgc gttgctgttt tgggggtggc
     9781 tctgggggat ctggggactc ttgtttgcca ttcccatcac tggaatggtg aaggtgtttg
     9841 ccgagcgcgt cgaagacctg cggccggtgg cggagttgct gagtgattga tccaccggtc
     9901 acatcacacg cgcggatcac cgccatgatc cgaggctgtt tcccctatct ttgtggcctc
     9961 gtcatcgtcg cggccttttt cgtcaccctt tgtgaaacgg aggcccgtcc gatcgcggta
    10021 cgatttctcg aagggttgtc gcacggattc cttctcgttc ggtcctctgc cggcgagatc
    10081 attggcgagg gcgagctgac ccaggtgatg aaagaaagtg gtgtgacgga aagccgtctg
    10141 gtgttccgct tcaaagatgg gtcgctccat gacgaaagag tcgcgttttc acaacagcgg
    10201 gtgtttaccc tgattcgcta tcaactggtg cagcgaggac catcttttcc cgagcagatc
    10261 gatgtgacag tcgatcgagc gacatccaaa tatgaagtcc gatcccgagt gcgagaaaac
    10321 gacaacgaaa aagtcctggc aggagagatc gatctgccta aggatgctta caacggcatg
    10381 attgtcacga ccctcttgaa cctcccgaag gcctcaagcg aatcagtgca ggtccttgcc
    10441 tttacgccga agcctatggc catcccactc gaattgagct tcgtcggtga acaagtcata
    10501 cgggtgggag agcagtccag gaaggcattg caatacacct tcaaaccgga tatcggccca
    10561 atcaaaaaat ttctagggaa ggtgctgggg aagttgccgg ctgacttcca ttacgattgc
    10621 ctcattctgg ccgacgacgt tccgagcttc gtgcggttcc aagggccttt gcaactgatg
    10681 gggccaattc tgaccattga actgacaagt cctcaagcgg cggaaacgcc gggggaccag
    10741 aagcatgtgt ccaaataacg ggcgagcctc acatgaatcc gattttcagc ctgttgatca
    10801 agcagcttcc aagactactt ccggtcgtga catccatgct ggaacgacca ccgtcgagaa
    10861 acacggaaga cgggcgactg gcaggcatcg aacaatccat agaatggctt gccgaacgtt
    10921 cagatgcgat cgaacggcga ctcaaacgac tcaccatgtt ggttgtcgca ggactgcttc
    10981 tctccgtcgt cgcgttcgtg ttgatgctgg tccgttagat ccgctagatc ccttgtccag
    11041 catccatgcg ggaattcagc aaaccatcgg agtcgctgac gcagtcaggg gtggccctct
    11101 cctcgtgaag cgccctgctc caggccagat agccgacata caccagggag ccatacaagg
    11161 cggatgccat atcgcgctgg ggatcccaga cgtctccctg atgtccaacc atggccttct
    11221 cgatatcggg atgtgccaac tgtccgatcc aggcttcgac gatttcccag aaggcgctaa
    11281 agcccaatac ggtcatcacg accagatagg gaagcaacca gcctttggca ttgcaccggc
    11341 gtcgaaacac ttcgaccagt ggcagggtca tcagaaaacc gaagctgaaa tgcacgatcc
    11401 gatcgaaatg attgcggtga aagtcaaacc accgatccag ccagtcaccg accggcacct
    11461 tgggataggt atagtgcact gcgactgtgt gcagcgtcag ccacagcgtg ataagggcat
    11521 aggatgtatt cgataaaggg aatgtgcgat gggtcagaac gagcacggcg acaaacgcca
    11581 ctggcagaat attcgacccc caccagattc tcggatcgaa gggcgaaatt gccaagaaca
    11641 aaaataacac cgcataccag gcgagcataa cttgtgggat cagagacttt gatgcggtcg
    11701 tcaacgagtc attcgatgca gtcatagctt tacgcctcac tttcagacaa acaggtcatg
    11761 gaatggggat aaaacacctt accacaggga tgttccgacg tgtagtgaga ggtctttcac
    11821 ctaagggatc agtgacagtg cgtgtcttgg tactcagctc ttggcttgcc ggttgtgcat
    11881 ccacaccgac tccgctcaca gtgccgtcca tgcgcatcga tgatccggcg tttcgcactt
    11941 cgttggaagc ctttgcaggg gcgcccatcc tcggtgacaa ccgcgttgac atcctcctca
    12001 acggagaaga gacatttccg gccttgctcg atgccatcag gtccgctcat catacgatca
    12061 cgttcgaagc ctacattttt cacgagggca agattgcaga ccagctcgtc ggcgctttcg
    12121 tcgaacgctg caaggccggt gttcgagtgg ccatactgct cgacgcccat ggctccgatg
    12181 ggctgcctga acggtatatc caggacctcc gtgacgcagg ctgcatactc gtaccggatt
    12241 ttcgtccctt aagcctctgg agcctcgaac ggacgaacaa gcggaaccat cgccgaatca
    12301 tggtcattga tggcctcgtc ggatttactg ggggatatgg agtggacgat acctggagcg
    12361 gtaacggacg tacggaagga cgctggcgag aaacgaacgt acggctgcaa gggccgatcg
    12421 tccaaagcct gcaggaagcc tttgtcgagc actggcgcga ggccactagc gtgctgctgg
    12481 gaggaaaaga ttactttcct tatccgcccg tgacggtcaa agacgtcccc gttctggctc
    12541 aggtcgtccg aagctcaccg ctgcaaggca acgacgccat gtaccgggtt ttcttgcagg
    12601 ccatttcctc agcccgcacg tctattctga tttcgacgcc ctatctctta ccgggtgagc
    12661 agttgaccga ggccctgttg gacgccgtcc gacggggcgt tcgcgttcgc gtcttggttc
    12721 cgagcgtggt gaaaagctcc ggggtcgaat tcgtgacgca ggcatcgcaa cgagaaggct
    12781 tcggagcctt gctggagggt ggcgttcagc tgcacgagta ttcgccagcc ctgctgcaca
    12841 cgaaaatgat gatcatcgac gggacgtggg cgacggtcgg cagcaccaac ctggacaacc
    12901 gttcaatggc catgaacgac gagctgaacg tcatgtttta tgatcgcacg attgcgaagc
    12961 ggctggagga aattttcgca gcggacgtcg cgtattcgca caaggtctcg cgcgaacaat
    13021 tggagaacca ggaatggctc catcgtgccc tgggcgtcct gctcagtccg tttcatgcct
    13081 ggttttgaaa caggctcatt catcgcgtaa gccctcaacg agcggttgtc tggctgccca
    13141 gcgcgcaggc agatagccgg ccaccccggc cgccccgatc gccagcaaca cggcttgaat
    13201 caacacttcc agcggaatcg tcatgcggat ggtccatcca aacgactgtt tattgaccac
    13261 cgcgatcagc acccaggcca gcgccaatcc ggccatgacg cccaacagcg cgccgatcaa
    13321 tcccaggtag atggcctccc acagaacgag ccgttccact tgcgccctgc tggccccgat
    13381 cgccagcaag gtggcgagtt cacgacgccg ctccatgacg gcggtgacga gggtattgac
    13441 gatgcccagg acggccacga agacggcaat cgcctcgagc acatatgtca ggacaaaggt
    13501 gcggtcaaag atcgtgagga tttctcggcg aaggtccttg ttcctgatga tgatgggcgg
    13561 tgccaccgac aactcgtggc cggccagtcc ttccgtgatc gagcgggcga cccgctcttc
    13621 gtccgcgtcg ggagagacat agatcccgaa aacggtcact cgatcgtcat cccaaaaacg
    13681 gcggtagacg cttcgttcca tcaccatctt tcccccgtcg gtcgcatagt cgtaaaacac
    13741 gccttcgatg ggaacggtgt ggggtccttt gggcgtggtg atggtgacaa ggctgcctgg
    13801 ctggaggcct aagcggttca cgaggacttc tgacacgagc actccacctg tatccgcagc
    13861 ccgcctgagc acgcttgccc cctccccttc caccatcaga taccggccgt gctcggcatg
    13921 gagacgtaaa tctcgtgaga tgagtgccac gggctcgccc tccacttcag cctctacatc
    13981 acgatacgcg tcaaccgccg ccaccccatt gagcgcctgt aggagaggca accagccagc
    14041 gggcaagctt cgcgaagcct gtcccgattg cttgctatgc aaccaggccg gtggcgcaat
    14101 gatgagatcc gccatgaccg tttcattgac ccaggcctcc accgtctctc gaaaactccg
    14161 caccatgatg accaccccga tcatgatggc caatcccacc aataatgccg atacggtcac
    14221 ggcatttcgg ccgggatgac gtgcggcatg atcggccgcc atgcgtctta aattggtgcc
    14281 cagcgacggg gcctccttcg ggtgctgcgt tgaccatcgc agtgccagga tgcacacggg
    14341 agccagaagt gcgaagccgg ctaggagaca caaggtcgcg gcatacccga aaatggggag
    14401 gcccttgaag ggaggtccta acgtgcacag cgctcccgct atcaagagcg ccaggcttcc
    14461 caataagtag cgttggcttc gcatggcgac cgacgtctca taatcccccg gcgccagcgc
    14521 tttcgttgtc accgttctgc tcgcgtcgat actcggtgcc actgcgcctg ccatcgacac
    14581 cacgattccg accaccagac cttgggccac ggcctcggcg ctctgtccat cgacgattga
    14641 aaacgctccg gtcacgccca acggcacata gagatcactg atcgtgcgac tcaccagcac
    14701 gaccatgctg cgtgccatcc agagaccagc aacaccgccc aagagtcccc ccagacaccc
    14761 gagtaatccg gcttcgagca ggaacaggcc ggcgacccgt cgctccgtca ttcccaaggc
    14821 gcgataaatc ccgatctccc gtcgtcgctg ggccaccgag aaactcatcg tgttgtacac
    14881 caagaagatg ccgaccaata accctaccca gctcagcacc gccaggttca actgaaacga
    14941 ccccaccatc ttctcgacct gcgccgtgcg ctgagccggc cgctggacca ccacagaagg
    15001 cgaaagcacc gctcgcaacg ccacggccac ttcctcgggc gttcgattcg gtgcggtgac
    15061 gacgtcaacg cggtccaatc ggccgaccat ttgaaacagc acttgggccg ccgccaaatc
    15121 catgatggcg acgcgattcc aataggattt gggccggtcg ctgccctgta ctagccccgc
    15181 caccttcagg ggcatccaac gcgagcctgc cctggccttg aacgtgcttc ccggaacaag
    15241 gttcaactcg tcgctcagct gacgcccgag gaacacacgg tccggctcca agagatcgtt
    15301 cagcaggtcg tcaccatcgg ctctctcgac acgaaaaccg aggctttcgg cttccgcgac
    15361 caggtccatc ccgtagacct gcacactttc accggcaggc gccccttcac caaggaccac
    15421 cgcttcttcg atgaggggaa tcactctttg gacgcccggt gcgtgtcgaa taacctcgag
    15481 aacggtttca tcgagtccga gatcgcggcc cgacacttcc agactcgccg ggccggcgac
    15541 ggtcaaaacc gctcgttcaa aagcccgaag cacctcacgg ttggccgtga ggatcgcgac
    15601 ggaagacaag acccccaagg cgacacccgc caccgtcagc gccgtcctga acggccgctg
    15661 ccacccatgg gctccaatca gcatgcccac caacctgagc caggctgtca tagtcgattt
    15721 tggcatacgg gtaagtgcgt agctacaaat ctgttgagac ggcgctttac cagtgtggat
    15781 gcgacaccgc gaggatctgc acaaatcggc agtttcgttt acagagtgcc attcctttgc
    15841 tagactcgct tcaagttatc gctggctatc gaaggtgggg aaacatgaag accgcgttac
    15901 gacgaaaagt tgacacgctg atcgaggaat ccgttgatct cgaattgttg acgcctcgcc
    15961 aacgagacat cctgcgtctg gtctctatcg ggcatacgaa tcgcgagatt gcaaatatgc
    16021 tgcagattag cgtgcgtacg gtggaagttc atcgcttcaa tctcatgcga cgattgaacg
    16081 tacgcaatgt ggcgcaactg ctcagacgag ccctccagct gggcttgctt tccaaatcct
    16141 tcgcaaaata actttgcggt cccacgaccc gctctactgc actccgcccg atagttctcg
    16201 atgctcctcc ggtgcgccgg ttctgctaca actccttgag cttgccgcgg cagtccccaa
    16261 tcgttttata gccttttttt tccaaacagg ccgtaagctc gtgctccaag cgtgcaaaga
    16321 cttggacgcc ttcttcaact aataccgtcc cgatctgtac cgccgaggcg ccgcagagca
    16381 catgctcgaa ggcatcgacg ccgtccacga caccgccggt tcccacgatc ggcatagtgc
    16441 cgtggaagtg tttccaaaag gctcgtacgt tagccagtgc gaccggttta atcatcgctc
    16501 cgccgagtcc gccaaagccg cctttgggct taataaccac ggtttccgcg tctggattga
    16561 cgacaaggcc gttgccgacg gaattgatca aagtcagaaa atccactccg caacgagcga
    16621 gtacatcgcc catcagtttg tgatgagccg ggtcaaaata gggcggcaac ttcaccccca
    16681 tggggaccgt cacgatcttc cgtagccgtt tgacgatccg ttcggaggta tccgcatcgt
    16741 agcctatttg cggtttgccc ggaatgttcg ggcaggaaag attgacttca atgagatccg
    16801 gttttgcgtg attaataacc tgagcaatgg ttgggaaatc ctcttcacac agccctgcca
    16861 cactggcaat gaccggcttg ccgaactgct tcagtgcagg gatcaatgcc gcgtaggcct
    16921 tgtagccgag gttcggcagt cccatggagt tgattgagcc accggggaac ccgtgatagc
    16981 gcggcatggg attgccttgc ctcgcttcga tcgtcatgga cttggtcacg atggctccgg
    17041 aatcagagcc gcccagtgcc atgagttcat cctcggttac gcacaaggcg ccggacgcgt
    17101 tcatgaaaca cgaggggaat ttcacgcccg caatgtcgac tgtaagatcc atcacagtcc
    17161 tactgcactc cgctcaccca ctccgttcag caacttgccg cgctgcgttc ggtgcgcgtt
    17221 aacaatcgtc cgtctttcat cgcccagata taatcggcgg cctccgctgc agctgcacta
    17281 tgcgtaacca gaagcaccgt aagctggaag tctctcgcca aggtgcgtag aagcgagata
    17341 atttcgcccc catgctgcga gtccaggttg ccggtcggtt cgtcagccag cacaatccgg
    17401 ggctgatgca caatcgcgcg tgctaccgct acccgctgtt gctccccccc cgacagctca
    17461 ctcggtcgat gtcgcgcgcg atgattcatg cggacgaggg ccaatacttc ggaaactcgt
    17521 tcagcaactc tgcggcgttc atcgcctcgc agcagcagag ggaaggcgac attttcttca
    17581 acggtcagac cgggaacaag atgaaaggct tgaaagacga ttccgatgca ctgacggcga
    17641 actctcgtcc agtcgtcggc gttgtagtgg gcggtggatt gtccgttcag ttgaatgtcc
    17701 cccgatgtgg gaaggtcgag ccctgcaatc aggttgagca acgtgctctt gccacatccg
    17761 ctggggccga caaacgcgca gaaagcaccg gctgtcacct ccaacgtgac ccgatccaac
    17821 ccgcagatcc ggtcgtcgcc ccgttcgtag agcttggaca cctccgtcag cttcactatc
    17881 ggcgcaatta acgggggcaa cattcgtgtc agacctcgtg ttctatacca taccccttca
    17941 cggagcgaca accttgacag ccgtcgccgg ttctcctacc ttcagcctga tgcgtgacca
    18001 catgctgaga tggtgccggc tggcgctccg cattgttctt cccgtcgact gcatcagttg
    18061 tgaccagccc ttgcggacgg atcaagcccc tcgtttctgc gatgcctgtt ggcagcgcat
    18121 ccatccgctc tcaggccctc gctgctccca ctgcgatcag ccgttcgtgg ctgcagctgc
    18181 cacgtcctgg actcccggtc acacgtgtca gcactgccgc gagcatcccc ctgcttatca
    18241 tcgggcctgg acgctgtatc cttacatccc accgttgcag gacgcgatct gtgcattgaa
    18301 gtaccggggg gtatcggcgc tggctggacc tctggcggga ttgatgatcc gcgcactccc
    18361 gccggacatc gagtgcgatg ttatcatccc cgttccgttg cacccgtcga ggctgagagt
    18421 tcgagagttc aaccaatcgc ttttgcttgc cgacaggctt gggcgctttt tcatgcggcc
    18481 ggtatccatg accagcctca tccgcactgt tgccacagag cctcaaacgg cgctgacgcg
    18541 ccgccagcga ttgcggaatc tccgccgcgc atttgccgtg cgagacgccg agcccctcgt
    18601 cggccgtcga attttgctgg tcgacgatgt ctataccacc ggaacgacgc tgaacgagtg
    18661 cgccaagacg ctgctagagg ccggggccct ctcagtctcg gcgctcacgc tcgccaggac
    18721 acttgactcg agccttgtac cagaccgtat ttgggccgac cacgctgacc gctcgttgac
    18781 aggattcgga ctttgacagg atgccgattt acgaatatcg ctgtgccgag tgccacaaac
    18841 ggagcagttt tctcgtcctg agccgctcca ctgaaagctc gctccgctgc tcccattgtc
    18901 agagtctcag actcgaacgg ctcttgtcgc gcttcgcgtc gccgaaatcg gaagaggcgc
    18961 ggatggaatc cttagccgac gacagcgcac tggccggtct tgatgagaac gatcccggaa
    19021 gcatggagcg tttgatgaaa cggatgggca atgagatggg agcagaagcc ggggatgatc
    19081 tggaagccat ggacaattct gatgaaggcg tcggtgatgg ggacggtctt gacagcctat
    19141 gacaggtatt gtcatacaaa ttccttgaca gatgtcgttg atttatttac cctgacgcca
    19201 attggagtgt aggcatatgg tgccggcgag cccgtggcgg atgttcaatg accaaagcgc
    19261 ccaagagcga gctcatgacc gcggaagaga cctgccgcta tctcaaaatc acttccagaa
    19321 cgctgtatcg ctatcttcac aatcatcaaa ttcccgcttt caagcttggg aaagaatggc
    19381 gattcgtccg ttcagatttg gagcagtgga ttcgagaccg gactcgggga cccgtcccct
    19441 cgtgaaggag cgcgttcgct gatgtttgcg ggagaatacc tctgcaaagt cgacgagaaa
    19501 ggacggttca tcgtcccatc gcccattcgc gagcaagtcg actcagaagg gcaggcagtg
    19561 gtgttcctca aaggcccgga agagtccatc ctgatctatt caatgaagga atgggagaaa
    19621 gtgctcgagc ggacgaaaac caccctcgat gaagagcaga gccgactatt catgcacttc
    19681 gtcgtctccg aagccgggac gtccgaactc gacaaggccg ggcgaatcct tattccagga
    19741 aagttgcgaa agctggtgcc gctggacgat gaccaggaaa tcatcctcgt cggactctac
    19801 catcgtctgg aaatttggaa tcccagcgaa tggcgccgct acattgctcg ctccgaaggt
    19861 cgctatgaac aaaacatggc gaaaattctt aacctcctct aggcacgcgt tgaaccttcc
    19921 tacgacttcc cgagctaggc cacttcgaga cacagcgtga actgatgccc ggctatcggc
    19981 gcccgatccg aattccggct tccagccttc cacccacttt ggtctctttt cgcacctcga
    20041 gaagcattcg ccgtccagcg caaccccagg ctgccatcct gactcacctg aatgctgcgg
    20101 ccctctcggc tcttcatgaa gagcaacggt ccatcaccct cacgcttccc gttcctccca
    20161 gcatcaacca tcaatatgcc acggtgaacg ggcgccgtct gctctcatcg accggccgat
    20221 cgtataaggc acaggtcgga caactcgtct ggatacggct cgcagcatct cctcataaga
    20281 ccgtcctctt gtcccaactc caatcctctg cgctcgcctt gtccatccgc ttctatttta
    20341 cgtcggcctt gagacgggat gtggatggcg gcttgaaaat tgcccaggat gccatctgcg
    20401 aaggtctcgg tttaaatgac aatcggatta ttgaaacgca cctctacaaa cacgtcgacc
    20461 ggagaaatcc gcgcatcgaa gtggtgttgt ccgccacttc gctggcgtga tcgtacggtt
    20521 attcgtatcg gagcgcttca atcgggttga gtcgagacgc cttgttggcg ggatagagac
    20581 cgaaaaacag tccaacggcg agagaaaaaa agaaggcgat caggatcacg tctgtggaaa
    20641 tgacggtggg ccatccagcc actacggtcg tcagttttgc tcccacgacc cccagcacga
    20701 cacccactgc tcctcccaag agacttaagg tcatcgcctc aatcaggaac tgcatcacga
    20761 tgtgacggcg cttggcgcca accgccatcc ggacgccgat ctcccgtgtc cgttcagtga
    20821 cggacaccag gagaatgttc atgatgccga tcccccccac cagcaaggat acggaagcca
    20881 ccgaaaacag caacagggtg agcgtgtagt tcgttccttc ctgcactttt ccaatgtcga
    20941 cctgcgtctt caccgtaaaa tcgtcgccct gttcagcctg aagccgatga cgtccgcgca
    21001 acacctcgcg aattcgctcc acggcttccg ggaggtcttc aggccgatcg gttgacgcga
    21061 acaaggctgc gaccgagccc aagaacatcg tgccgaagac tttccgctct gcggtcgtaa
    21121 aaggaataaa gatgacgtca tcctgatcgg atccctgcgc cgactggcct ttcggcgaga
    21181 ggacaccaat gacgcggaac gggacgtttc taattcggat cactgcgccg accggttctt
    21241 cgccttcatc aaacaggttg tccacgatgg tttgaccgat caaggccacg cgggcagcgc
    21301 tgtcgagatc cgcctgagtg aaaggccctc cgctcgaaaa cgcccaatcc cgaatcgtga
    21361 ggtagctcgg cgacacgccg ttaatcggac cattccagtt ccggttcccg ttgaccatct
    21421 gcatgacttc tcgccttgcc catcccgtct ccgacagcaa cggaatcttt ttcttgagat
    21481 ccaacgcatc cgagacggtg agggtcacag ccccgccttg gccgccgcgc accccactca
    21541 ccgtcgttgc gccgggccaa acgatgatga cattcgtccc catcgtggca atttgggcct
    21601 gcacagcccg cttcgctcct tctcctatgc tgaccatggc gatgactgcg ccgactccaa
    21661 tgacgattcc caacatggtc aacccagcac gcaggcgatt acgggccaga atgcgcaagg
    21721 ccgtcatgac ggtgagccaa ataaaggccg gcatctatac ctcgacggat tgtgcactcg
    21781 gtttcttcat tcgatcgctt aagactttgc cgtctttcaa cacgatctcg cgccgtgcgt
    21841 acgcggcgat gtccgcttcg tgcgtgacga gaataatcgt aatgccctcg ccattcagt
//
LOCUS       DASSCQ010000158        10816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_470630, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000158 DASSCQ010000000
VERSION     DASSCQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10816
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470630"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /gene="alr"
                     /locus_tag="VFH05_11080"
     CDS             complement(<1..998)
                     /gene="alr"
                     /locus_tag="VFH05_11080"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET6678806.1"
                     /translation="MHTTYASIDLAALGRNLSRLQTRLHPTCGVMAVVKANAYGHGAV
                     EISRALMELGISNLAVASVQEGVALRHAGITADIVVLADLFDEHCEDLFAYRLKPVIN
                     DLRLLPALAKKAETTQHPFPIHLKIDTGMGRLGFPPSEFERVLDALPSWKSLTIEGLM
                     THLADSDGEETGHTERQLELFRTMLDGCKRRGMMVPMIHAANSAALIRYPHSHFSLVR
                     PGIALYGYHTLPQAVQCDELHPILSLRSTVMQLRTIKPGDSISYNRTFIAKRPSIIAV
                     LPIGYADGYSRKLSNKGCVLIKGRRAPIVGLVCMDMTMVDVTDLPSVRIGDAVTVIG"
     gene            complement(1117..1680)
                     /gene="frr"
                     /locus_tag="VFH05_11085"
     CDS             complement(1117..1680)
                     /gene="frr"
                     /locus_tag="VFH05_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET6678807.1"
                     /translation="MSNSAAIRQAVTGKMEGALEHLKRDLAGLRTGRASVALLDNVRV
                     DYYGTMTPLRQVANLSTPEARLITIQPWEPQLIKEVEKALSISGLGITPSNDGKIIRV
                     PLPPLTEERRKELSKICKKHGEDTKVHIRGFRRDANEELKKLQKDAKLTEDELRKSEA
                     EIQKLTDQYVQKIDDAIKKKEQEILEV"
     gene            complement(1698..2423)
                     /gene="pyrH"
                     /locus_tag="VFH05_11090"
     CDS             complement(1698..2423)
                     /gene="pyrH"
                     /locus_tag="VFH05_11090"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET6678808.1"
                     /translation="MSSAKYRRILLKVSGEMLAGEQGYGIQPSVLEGLAAEIAEVVSL
                     DIQVAVVIGGGNIFRGIAASASGMERASADYMGMLATVLNALALQNALERAGVMTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKKRVVIFAGGTGNPYFSTDTAAALRAMEIGAQVIMK
                     GTKVDGIYDADPAKNPGAKKYTEISFLSILNQHLAVMDSTAISLCMDNQLPLVVFNLK
                     IPGNFKRVALGEQIGTIVTVDKR"
     gene            complement(2420..3016)
                     /gene="tsf"
                     /locus_tag="VFH05_11095"
     CDS             complement(2420..3016)
                     /gene="tsf"
                     /locus_tag="VFH05_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET6678809.1"
                     /translation="MAGASQLVKELREKTGAGILDCQKALQESGDDIEKAIDYLRQKG
                     LAAAQKKSGRETNQGLIHAYIHMGGKIGVLIEVNCETDFVARNEEFKAFVNDLALQVA
                     AAKPSYVKREDIPKEVVDKERAIYEGQAKEMGKPPAAWPKIVDGKLEKFYQESCLMEQ
                     AFIKDPAVMVKDLLAQKIAKIGENMNIRRFTRYQLGEA"
     gene            complement(3074..3847)
                     /gene="rpsB"
                     /locus_tag="VFH05_11100"
     CDS             complement(3074..3847)
                     /gene="rpsB"
                     /locus_tag="VFH05_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET6678810.1"
                     /translation="MGVVSIKELLEAGVHFGHQTNRWNPKMKKFLFGERNGIYIIDLQ
                     QTVARMEQAYAFVRDTVASGQSVLFVGTKRQAAEILQEEAVRSNMFYVNQRWLGGMLT
                     NFQTIRRSIDKMKKLEGMLADPAQYGLTKKEIAQKQKQIVKLQKFLTGIKNMRGLPGA
                     VFVLDTRVERIAVQEATRLEIPVLAIIDSNCDPDNITYPIPGNDDAIRSIKLITSRIA
                     DACIEGAHLRTQRDEAEFHSSPAAEDKAPTASLAGAPAS"
     gene            complement(4077..5285)
                     /gene="argJ"
                     /locus_tag="VFH05_11105"
     CDS             complement(4077..5285)
                     /gene="argJ"
                     /locus_tag="VFH05_11105"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET6678811.1"
                     /translation="MSQNDSRGITAPLGFRAAGLHCGIKKAGVLDLALVVSDRSGPIG
                     GVFTTNRVVAAPVALDRLHLRHGVGRAILINSGNANACTGRPGMAAAQRTAAVVARTL
                     GIPIHEVFIGSTGVIGRPLPIDRIIKAVPALVERVSSLGGLSAARAILTTDLKPKAIA
                     RRARINGRTVIIGGMAKGSGMIHPAMATMLGYLTTDAAITRTALQRALTQAVDDSFNC
                     ISVDGDTSTNDTVLCLANGLAGNRPITEGTSAFRQFVRLLTEVCQPLALAICRDGEGV
                     TKVVRIEVEGARTTLQARQVANTIGTSNLVKTALFGEDANWGRVMAAIGRAGVTMDPA
                     KINVSFGGVPMVRNGVGLGPAAERRIAKVFHRKEFTIQVKLGQGRHRSHLWTTDLSYD
                     YVRINASYRS"
     gene            complement(5318..6391)
                     /gene="argC"
                     /locus_tag="VFH05_11110"
     CDS             complement(5318..6391)
                     /gene="argC"
                     /locus_tag="VFH05_11110"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET6678812.1"
                     /translation="MRKIRIAIAGASGYAGAELVRLAALHPYFEVVIVTSEKSAGTPV
                     ATVFPSLAGTVALTFQSLAPEALAQQADAVFLALPHTASMAPVAACAETGTFVVDLSA
                     DYRLKDPAAYERWYQTAHTHPQLLKSAVYGLPELHREAIAKAKLVASPGCYPTAAILQ
                     LAPLFAQDLVQPHTVVIDAKSGISGAGRSPALPYHFPEAHESLEPYKIGQHRHIPEIE
                     QELQALLPRQAGSTKVDPDSGTVTFTPHLVPMNRGILSTAYCRLKKPLPLADVRALYH
                     TFYKNDRFIRLSDDIVPNPRYIKGSNYCDIGVYADQRAGWLVTVAALDNLVKGAAGQA
                     IQAMNLMLGLPEETGLTAPAAYP"
     gene            complement(6610..7002)
                     /gene="rpsI"
                     /locus_tag="VFH05_11115"
     CDS             complement(6610..7002)
                     /gene="rpsI"
                     /locus_tag="VFH05_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET6678813.1"
                     /translation="MAVATEYATGRRKCAIARAWVTATAGDITVNDQPLEKAFPPLTL
                     RQIIQLPLEMAGVTGKFSIKATVHGGGPTGQAGALRHAIARALVSLSQPLRAPLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            complement(7040..7471)
                     /gene="rplM"
                     /locus_tag="VFH05_11120"
     CDS             complement(7040..7471)
                     /gene="rplM"
                     /locus_tag="VFH05_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET6678814.1"
                     /translation="MSTYLQKPADVQEKWYLVNAEGKTLGRLAARVAGMLRGKHRPTF
                     TPNVDMGDHVVIINAEKIHLSGDKMETKLYRSHSGYPGGLKTATAKHVFRKDPTRLLV
                     AAIEGMLPKTPLGNHMAKKLRVYVGATHPHQAQNPEPITLN"
     gene            complement(7516..8013)
                     /locus_tag="VFH05_11125"
     CDS             complement(7516..8013)
                     /locus_tag="VFH05_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET6678815.1"
                     /translation="MEQPPSSPDQGLIAYKVDRVLDETNTDTKILVLSRQDGQSDTFP
                     IWIGAAEGHAIKLALDTTLTPRPMSHDLIKSIAEHLNVHVRRIVIGDVKNSTYYATVY
                     LENNGVERTVDARPSDAISLALRTNAPLYITEDVLARRNTANLDLWLAKQDKNSMNQQ
                     EVRES"
     gene            complement(8013..8468)
                     /locus_tag="VFH05_11130"
     CDS             complement(8013..8468)
                     /locus_tag="VFH05_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET6678816.1"
                     /translation="MITQMHLKGLMFDPYNNAYIVVLRDEEGHEMLPIWVGKAEAGAI
                     SMALENVAPPRPMTHDFMKSVLDAYDAKVISVVITDLNENTYFAKIHLMYEDSEYTVD
                     ARPSDAIALALRSNAPVFAKETVLNKQSSEELEQWLENLKPEDFGKLDS"
     gene            complement(8628..8909)
                     /locus_tag="VFH05_11135"
     CDS             complement(8628..8909)
                     /locus_tag="VFH05_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HET6678817.1"
                     /translation="MADVQCVTCGQTGEPITDPLFMGKLETEIKAKVCKPCWKKWEAM
                     RVMVINEYQVNLGDESGRELVRKQMKAFLKIGEEQADTAKLDQNYRPQS"
     gene            9042..9128
                     /locus_tag="VFH05_11140"
     tRNA            9042..9128
                     /locus_tag="VFH05_11140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9076..9078,aa:Leu,seq:caa)
     gene            9327..10091
                     /locus_tag="VFH05_11145"
     CDS             9327..10091
                     /locus_tag="VFH05_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678818.1"
                     /translation="MRKVLALGAAVLFVGSVSVAVAQERLQSNQSGMGVGEGVGDVSG
                     NSTRVNVFDRTSFLERLSQAETVYGRVLGVDLPGRKLHLESGGSSHDEGRAGGGAMNY
                     RTVYFDDKTNMDQVRALEAGDDIILQVFEETTQQQPFGTGRKLVREISVMRGNEKLAG
                     FGGLGQRPNPMTERGIVTNSGGITGGISGGVLPGTAQGGSISTTIGEFTGSAPCWNCE
                     PQPGWGYQTVAPETKVQNKSDYGTEVSKPNLVKGLQ"
     gene            10223..10483
                     /locus_tag="VFH05_11150"
     CDS             10223..10483
                     /locus_tag="VFH05_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET6678819.1"
                     /translation="MGKDVNEQQEAVQPHAELDLRGVICPYNFVKTKLKLETMEPGQI
                     LSVILDAGDPIRNVPMSVRNEGHEVLSQEPLQQSYRVLIRRS"
     gene            complement(10456..>10816)
                     /locus_tag="VFH05_11155"
     CDS             complement(10456..>10816)
                     /locus_tag="VFH05_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678820.1"
                     /translation="AALSGYSGDRLIFLGRLPRTRPLLDQLCSTLKREPATTIMFVHP
                     PILPHVLNSIGRVLPNRAVTLAVNMTRKDERLCQGSATSLLEIARSLSRNAELTLVLA
                     GARERPGTTQLRRINTR"
BASE COUNT         2138 a         2982 c         3210 g         2486 t
ORIGIN      
        1 ccgatcacgg tgacggcatc accgatacga accgacggga gatcggtgac atcgaccata
       61 gtcatgtcca tgcagaccaa gcccacgata ggagctcgcc ggcctttgat caagacacaa
      121 cccttatttg aaagcttgcg gctgtacccg tcggcatacc cgatcggcag cacggcgatg
      181 atcgacggcc tcttggcgat gaaggtgcga ttgtagctga tgctgtcgcc gggcttgatc
      241 gtccgcaatt gcatgaccgt actgcgaagc gagagaatgg gatgcagttc gtcacactga
      301 acggcttgcg gcaaagtgtg atagccgtac agcgcgatgc ccggcctgac gagcgagaag
      361 tgcgagtgcg gataccgaat aagggcggcg ctattggccg catgaatcat cggcaccatc
      421 atgcctcgtc gcttgcatcc gtccagcatc gttcggaaca attccaactg ccgttcggta
      481 tgaccggtct cctcaccgtc gctgtccgcc aaatgggtca tcaggccttc aatggtgagc
      541 gatttccatg aaggcaaggc gtccagcacc ctctcgaatt ccgacggcgg aaatccgaga
      601 cgtcccatcc cggtatcgat cttcagatga ataggaaagg gatgctgggt tgtctcggcc
      661 tttttggcga gcgccggcag aaggcggaga tcgttgatga caggcttgag ccggtaggcg
      721 aaaaggtctt cgcagtgttc gtcgaacagg tccgcgagga caacaatgtc ggcggtgatg
      781 ccggcgtgac gcaaggccac accttcttga accgacgcca cggccagatt cgaaatcccg
      841 agttccatca gcgcgcgcga gatctcgacg gcgccatggc cgtaggcgtt cgctttgacg
      901 acggccatga ccccgcaggt aggatgcagg cgggtctgaa gtcgtgagag attccggccg
      961 agcgcggcga gatcgatgct ggcgtaggtg gtgtgcaagg ttacttacgc tggccgggtg
     1021 aaaaggcggc catgttgacc ggccagcacc tgttcacaaa gggataatga tagcgttctg
     1081 acgttcagag ggaccggagg atccgatgag tcgtgatcaa acttcgagga tttcctgttc
     1141 cttcttcttg atggcatcgt cgatcttctg cacgtactga tcggtcagct tttgaatttc
     1201 cgcttccgat tttcgtaatt cgtcttcggt caatttggca tctttttgga gtttcttcaa
     1261 ctcttcgttg gcatcgcgac gaaatccccg gatgtgaacc ttggtgtcct cgccgtgctt
     1321 cttgcagatc ttgctgagtt ccttgcggcg ttcttcggtg aggggaggca gcggcacgcg
     1381 aatgatcttg ccgtcgttgg acggcgtaat gcccaacccg gagatggaga gcgctttctc
     1441 gacctccttg atcagttgcg gctcccaggg ttgaatcgta atcagccgag cttccggtgt
     1501 cgagagattc gcaacctgcc tcaacggcgt catcgtgccg taataatcca cacgcacatt
     1561 gtcaaggagc gccaccgagg cccgtcccgt tcgcaagccg gccaaatccc gcttcaagtg
     1621 ctccagcgcg ccttccatct ttcccgttac agcttggcgt atggcagctg agtttgacat
     1681 aacgactccg caagaaatta gcgtttatcg acggtgacga tcgttccaat ctgctcaccg
     1741 agggctaccc gcttgaaatt cccggggatc ttcagattga agacgaccag cggcagttga
     1801 ttatccatgc agagactgat ggctgtcgag tccatgactg cgagatgttg gttcaggatc
     1861 gaaagaaacg agatctccgt atatttcttc gccccgggat tcttcgccgg gtcagcatcg
     1921 taaatgccgt caaccttggt gcctttcatg atgacctggg ccccgatctc catggcgcga
     1981 agcgcggcgg cggtatcggt cgagaagtag ggattgcccg tgccgccggc gaaaatcacg
     2041 acccgttttt tttccagatg gcggatggcc ctgcgacgaa tatacccttc ggccagctgc
     2101 cgcatctcga tcgcagattg gacgcgcgtc atcacgccgg cgcgttccaa cgcgttctga
     2161 agcgccaacg cattgagcac cgtggccagc atgcccatgt agtcggcgga ggcccgttcc
     2221 atcccggagg cgctggcggc aatgccgcga aagatattgc ccccgccgat gacgactgcc
     2281 acttgaatgt cgagggaaac cacctcggcg atttcggcgg cgaggccttc gaggacggag
     2341 ggctggatac catagccctg ctcaccggcc aacatctctc cactgacttt gaggaggatg
     2401 cgccggtatt tcgcagagct catgcttcgc ctagttgata gcgtgtgaag cggcggatgt
     2461 tcatgttctc gccgatcttg gcgatcttct gggcgagcag gtctttgacc atgacggcgg
     2521 ggtccttaat gaacgcctgt tccatcaggc agctttcttg atagaacttt tccagtttcc
     2581 cgtccacgat cttcggccag gcggctggcg gcttgcccat ctctttggcc tgaccttcat
     2641 aaatggcgcg ttctttgtca acgacctcct tggggatgtc ctcgcgcttc acgtatgagg
     2701 gcttggcagc ggcgacctgc aaggccaggt cattgacgaa ggccttgaat tcttcgttcc
     2761 tggccacaaa gtccgtttca cagttgactt cgatgagtac tccgattttg ccacccatat
     2821 gaatgtaggc gtgaatcaac ccctggttgg tttcccggcc ggacttcttt tgggcagcgg
     2881 ccagaccctt ttgtctgagg taatcaatgg ccttttcgat gtcgtcgccc gactcctgca
     2941 gcgctttctg gcaatccaga atgccggcgc ccgttttctc tctcaattct ttgaccaact
     3001 gacttgctcc agccatgcgc aatcgtcctt cttcgtggaa tcaatggatg tgtgcggtcg
     3061 ctcgaaacga tggttatgac gcgggggcgc cagccagcga tgccgtcggg gctttgtctt
     3121 cggcggcggg actcgagtgg aattcggcct cgtcgcgttg cgtgcgcaaa tgggcgcctt
     3181 caatgcaggc atccgcgatg cgcgacgtaa tgagcttgat cgagcgaatc gcgtcgtcat
     3241 tgccgggaat cgggtatgtg atgttatcgg gatcgcagtt gctgtcgatg atggcgagaa
     3301 ccggaatctc caaccgggtc gcctcctgga ccgcaatccg ctcgacccgc gtatcgagca
     3361 cgaagacggc gcccggaagg cctcgcatgt tcttgatgcc ggtgagaaac ttctgcagct
     3421 tgacgatttg cttctgtttt tgtgcgatct cttttttggt caggccgtat tgggccggat
     3481 cggccagcat gccttccaat tttttcattt tgtcgatgct gcgccggatg gtctggaaat
     3541 tcgtgagcat tccgccgagc caccgctggt ttacatagaa catgttcgag cgaacggctt
     3601 cttcctggag gatttcggcc gcttgacgtt tggtgccgac gaagagcacg gactgtcctg
     3661 aggcgacggt gtcacggacg aaggcatagg cctgttccat tctggcgacc gtctgctgca
     3721 gatcgatgat gtaaatgccg ttgcgttcgc cgaagaggaa tttcttcatc ttcgggttcc
     3781 accgattggt ctggtgtccg aaatggacac cggcttccaa taattccttg attgacacga
     3841 ctcccatacc ttcacctccc ctcaaggcct gcagagcgcc gtgtgacggg cgagggagtc
     3901 cgcctccctt attctcaagc caagcgacgt caaagacgcc gatgtggatt ttaataattt
     3961 cccctctcgt tcgccgtgag acggaagagg gggcctggat acgtcagccc gaaagccttg
     4021 gaacgttagc acaatgcccc cgagtttaca aggtttcaag gtgaaggcaa gtggcctcat
     4081 gaccggtaac tggcattgat tcgcacgtag tcgtaggaca agtctgtcgt ccacagatgg
     4141 gaccggtgac ggccctgtcc cagtttcacc tgtatcgtga actctttgcg atgaaagacc
     4201 ttggcgattc ttcgttctgc ggccggtcca agaccgactc cgttcctgac catcggcacg
     4261 cctccaaagg acacattgat ctttgcggga tccatggtga ctcccgctct accgatggcc
     4321 gccatgacgc ggccccagtt ggcgtcttcc ccgaacaggg cggttttcac caggttcgac
     4381 gtgccgatcg tgttggcgac ctgcctggct tgtagggtcg tacgggcacc ctccacctcg
     4441 attctgacga ctttggtgac cccttcaccg tcacggcaaa tcgccagcgc caacggctga
     4501 cagacctcag ttaacaggcg cacaaactgc cggaaggccg aggttccctc cgtaatggga
     4561 cgattgccgg ccaagccgtt ggcgagacac agcaccgtgt cattggtgct ggtgtctccg
     4621 tcgacggaga tgcaattgaa ggagtcatcg accgcctgtg tcagcgctct ttgcagggcg
     4681 gtacgggtaa tcgccgcatc ggtcgtgagg tagcccagca ttgtggccat ggcaggatgg
     4741 atcatccccg agcccttcgc catccctccg atgatgaccg tccggccgtt gatgcgcgct
     4801 cgccgggcga tggctttcgg tttgagatcg gtcgtgagaa tggcacgggc tgcgctcagt
     4861 cccccgaggc tactgacgcg ttcgaccaaa gcgggaaccg ccttgatgat gcggtcgatg
     4921 ggaagcgggc gtccgatgac tcctgtcgag ccgatgaaga cttcgtgaat gggaatgccc
     4981 aatgttcgtg cgacgacggc cgcagtgcgt tgggccgcag ccatgcccgg acggccggtg
     5041 caggcgttcg cattgccgct gttgatcaaa atggcacgcc cgacgccgtg gcgcaggtgc
     5101 aggcggtcga gcgcgaccgg tgccgcgacg acgcggttgg tcgtgaacac gccgccgatg
     5161 gggccgctgc gatcagacac aacgagcgcg agatcgagta cccccgcctt cttaatgccg
     5221 caatgcaggc cggcggcgcg aaagcccagg ggcgctgtaa tgccacgaga gtcgttctgg
     5281 ctcatggaga cctgtgcatt gaaggatgac cggagggcta gggataggcg gcgggcgcgg
     5341 tcaggccggt ctcttccgga agtcccagca tcaagttcat cgcttggatc gcctgcccgg
     5401 cggcaccttt caccagattg tcgagggccg cgacggtcac gagccagcct gcccgttgat
     5461 cggcgtagac accgatgtcg caatagttcg accctttgat gtaccgggga ttgggcacaa
     5521 tgtcgtcaga gagtcggatg aaacgatcgt ttttataaaa cgtatgatag agcgcgcgta
     5581 catctgcgag cgggagaggt ttcttcaagc ggcaatacgc tgtgctgaga atgccgcgat
     5641 tcatcgggac gagatgcggc gtaaaggtca cggtaccgga gtcgggatcc accttggttg
     5701 agccggcctg acgtggcagc agcgcctgga gttcctgctc gatttcagga atatggcgat
     5761 gctgaccgat cttgtagggc tcaagcgact catgggcctc gggaaaatga tagggcagtg
     5821 ccgggcttct tccggcgccg gagatgcctg acttggcgtc gatgacgacc gtgtgcggtt
     5881 gcacgagatc ctgggcgaag agcggcgcca attgtaaaat tgccgctgtc gggtagcacc
     5941 cgggcgatgc cacgagcttc gccttggcaa tggcttcgcg atgcaattcc ggtagtccat
     6001 agacggcgga tttgaggagt tgaggatggg tatgagccgt ttgataccag cgttcgtagg
     6061 cggccggatc tttcaagcga taatccgcac tcaggtcgac gacgaacgtt ccggtttccg
     6121 cacaggccgc gaccggagcc attgatgcgg tatgtggcag ggcgagaaac acggcatcgg
     6181 cctgttgagc cagggcctcc ggggcgagcg attgaaaggt cagcgcgaca gtgcccgcca
     6241 ggctgggaaa gacggtggcg acgggggttc ctgcggattt ctccgacgtg acgatgacga
     6301 cttcaaaata gggatggagc gcggcaagac ggacgagttc ggctccggca tagccgctgg
     6361 ctcctgcgat cgcgatccga atctttctca tagtggtgtg ccgatgcggc aacagccctc
     6421 cagctggatt ctcgcagcgc tcaaaccttc aacgtaccta cagccatacg cctcgggctt
     6481 tcgcttgttg cggccttgct ggaagaacgt gttcatcatc cttctgaata atcacagtgg
     6541 ttatagctgt agacataaaa aaagggaagg ccggaagcct tccctttttc cagatgctgg
     6601 ctccgacgct tagcgtttcg agtactggaa ccgctttctc gctccctttt ggccgtattt
     6661 tttccgctct tttacgcgcg agtcgcgggt gaggagacct tccttcttga gaggagcgcg
     6721 aagcggctgg ctcaaagaca ccagggctct cgcgattgca tgccgcagtg caccggcttg
     6781 tccggtcggt ccgccgccgt gcacggttgc cttgatggaa aacttgccag tgaccccggc
     6841 catctcgagc gggagctgaa tgatctggcg aagtgtgagc ggaggaaatg ccttttcgag
     6901 cggctgatca ttcacggtaa tgtcccccgc cgtggcggtc acccaagcgc gggcgattgc
     6961 gcatttcctt cgtcctgtcg cgtattcggt agctactgcc atgctctctc cttatttgtg
     7021 aggatgctga acaagccaat caatttagcg tgatcggttc cggattctgt gcctgatgcg
     7081 gatgggtggc gcccacatag acgcgaagtt tctttgccat gtggttgcca agcggcgttt
     7141 tcggtaacat gccttcgatc gccgcgacga gcagtcttgt cggatctttt cggaagacat
     7201 gcttggccgt cgcggtcttt aagccgcccg ggtaaccgct gtgacttcga tagagtttgg
     7261 tttccatctt atctccggac aaatggatct tttcggcgtt gatgatgacc acgtggtcgc
     7321 ccatgtctac gttcggggtg aaggtcggcc gatgcttgcc ccgaagcatc cctgccacgc
     7381 gggctgcgag gcgtcccaac gttttgcctt cggcattgac taggtaccat ttctcttgca
     7441 cgtcagctgg tttctgtaaa taagtcgaca tcgtctcgcc gcgctccttc acgccaaggg
     7501 atcacaattg aaagatcaag actcccgtac ttcctgttgg ttcatgctgt tcttatcctg
     7561 tttagccagc cacaaatcga gattggccgt gttccggcgc gcgagcacgt cttcggtgat
     7621 gtacagcggc gcattggtgc gcaatgcgag cgagatggcg tcgcttggcc gagcatccac
     7681 ggtgcgttcc acgccgttgt tttccagata caccgtcgca tagtaggtgc tgttcttcac
     7741 gtcgccgatg acgatacgtc gcacgtggac gttcaaatgc tctgcgatgc tcttgatcaa
     7801 atcatggctc atcggccgtg gagtcaacgt cgtgtcgagc gccagtttga tcgcatgccc
     7861 ttcggccgca ccgatccaaa tcggaaaagt gtccgactgg ccgtcctgac ggctcaacac
     7921 caggattttc gtgtcggtgt tggtctcatc caggacacga tccactttat aggcgatgag
     7981 tccctgatcg ggcgaggaag gcggctgttc cattatgagt ccagtttccc gaaatcttcg
     8041 ggtttcagat tctcgagcca ctgttcgagt tcttctgagc tttgcttgtt gagcacggtt
     8101 tccttggcga acaccggggc attggatcgg agcgccagcg caatagcgtc gctggggcgg
     8161 gcgtccacgg tgtattccga atcttcatac atcaagtgga ttttcgcgaa gtaggtattt
     8221 tcgttgaggt ctgtgatgac gacgctgatg accttggcgt cgtacgcatc gagaaccgat
     8281 ttcataaaat catgggtcat cggacgggga ggggcgacat tttccaatgc catgctgatg
     8341 gcgccggctt cggccttgcc gacccagatt ggaagcatct cgtggccctc ttcgtcgcgc
     8401 aggacgacga tgtaggcatt attgtaggga tcgaacatca agcctttgag atgcatctgt
     8461 gtgatcacag gaaacgaagc tccaccggct gttgggaaga gagctctcca gaccgcaaca
     8521 ggaactgtgc accctagcat ttttgaattt cgcatgtcaa cgaagccggc gacttcgctg
     8581 aagaaaccgc cgcacgttgt gacctggaga gttccgaaat acccgggtca agattgaggg
     8641 cgataatttt gatccaactt ggcggtgtcc gcttgctctt cgccaatctt gaggaacgct
     8701 ttcatctgtt tcctgacgag ttctcgtccg ctttcatcgc cgagattgac ctgatattca
     8761 ttgattacca tcacccgcat cgcttcccat ttcttccagc aaggtttgca caccttggcc
     8821 ttaatctctg tctcaagttt gcccatgaac aaggggtctg taatcggttc cccggtctgg
     8881 ccgcaggtga cgcactgcac gtctgccata agatatccgc ctcctctaat gtaggtggtt
     8941 cggccgaatt gtaccatcgg gtttccgtca attaaaaggg ggagctttca attgaccctt
     9001 gtgaaacctt cgtgttagaa gtggcgcccc ttcacttctc tgcccggatg gcggaactgg
     9061 cagacgcgcc ggactcaaaa tccggttctc gcaagagagt gggagttcga ccctccctcc
     9121 gggcaccacc agggtggctc atgagagcca gacggaagac ggatggtcct ctttaacctc
     9181 gccttcaaac cctcttgact tgttttttag actggcctat agtccggccc ctagtattat
     9241 gaaaatcaaa cgaattcatg gatcacccgt cgtaccatcc tgttgtctgt attatttcta
     9301 ttttctactc accaaggagg cagcacatgc gtaaggtgtt ggcgctagga gcggcagttc
     9361 tctttgtcgg ctccgtgagt gtagcggtcg cccaggagcg gttgcagagc aaccaatccg
     9421 gtatgggcgt cggtgaaggg gtcggtgacg tgtccggaaa tagtactcgc gtgaatgtgt
     9481 ttgaccggac gagttttctt gagcggttat cgcaggctga aacggtgtat ggccgagtgc
     9541 tgggagtgga tctacccgga aggaagttgc atctggaaag cggcggcagt tctcatgatg
     9601 aaggacgtgc gggcggcggc gcgatgaatt accgcacggt gtattttgat gacaagacga
     9661 acatggacca ggtgagagcc ctggaagccg gtgacgatat tattctgcaa gtgttcgagg
     9721 aaacgactca acaacagccc tttgggacag gccgcaagct ggttcgtgaa atcagcgtaa
     9781 tgcgtgggaa cgagaagctc gccggcttcg gcggtctggg tcagcgcccg aacccgatga
     9841 ccgagcgcgg catcgtgacg aacagcggtg gaatcaccgg cggtatcagc ggcggagttc
     9901 tcccaggcac ggcacagggt ggatcgatca gcaccacgat cggcgagttc accggttcag
     9961 caccttgctg gaactgcgaa ccgcagccag gctggggcta tcagaccgtc gcgccggaga
    10021 ccaaggtgca gaataaatca gactacggaa ctgaggtgtc gaagccgaac ttggtcaagg
    10081 gtctgcagta agccaagttt tgcctcgaat cgatgcgaag gggcagccgc aaggctgccc
    10141 ctttttattg gtctcagacc gacgggccca attgtccatt gcagccggtt gatgtccttg
    10201 atacgatttt tctgaacgcc tgatggggaa agacgtgaac gagcagcagg aagcggttca
    10261 acctcacgcc gaattagatc tgcgaggggt gatttgcccc tacaacttcg tcaagacgaa
    10321 gttgaaactg gaaaccatgg agcctggaca aatcctttcg gtgattctcg atgccgggga
    10381 tccgatcaga aacgtcccga tgagcgtcag gaacgaaggt cacgaggtgt tgtcccagga
    10441 gcctctccaa caatcctatc gcgtattgat ccgacgcagc tgagtcgtcc ccggtctttc
    10501 ccgcgcgcct gcgagcacca gcgtgagctc tgcgtttcgc gaaagagacc gagcgatctc
    10561 aagcagcgat gtcgcggatc cctgacacag ccgttcatcc tttctcgtca tgttcaccgc
    10621 cagtgtaacg gctcggtttg gaaggacacg cccgatgctg ttgaggacat gcggaagaat
    10681 cggtggatgc acaaacatga ttgtggttgc tggttcacgt ttgagtgtcg aacacaattg
    10741 atccagcagg ggacgtgtcc gtggtaagcg accgaggaag atgagccggt cgcctgagta
    10801 tccagacaag gcggcg
//
LOCUS       DASSCQ010000159         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_681032, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000159 DASSCQ010000000
VERSION     DASSCQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681032"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /gene="topA"
                     /locus_tag="VFH05_11160"
     CDS             complement(<1..1447)
                     /gene="topA"
                     /locus_tag="VFH05_11160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET6678821.1"
                     /translation="MAKSLVIVESPTKAKTISKYLGRGYTVMASVGHIKDLPTSKLGV
                     DLEHDFKPQYVTIKGKSKVLADIKKKAEESDKIFLAPDPDREGEAIAWHLSEELRGKS
                     KKKSDGKVFRVLFNEITERAIKQALQSPGQIDMKLVNAQQARRVLDRIVGYQGSQLLW
                     NKVRRGLSMGRVQSVAVRLMCEREAEREAFRAEEYWSIIVLLAGANPPPFEAKLHSIN
                     GEEASIGNAEQAGQIVSAIEGKAFAVTSIERKEKKRNPVAPFITSRLQQEAARKLHFT
                     PKKTMTLAQQLYEGIEIGSEGSTGLITYMRTDSPRISQEATADARQVIQERFGAEYLP
                     DVPNVYKTSKAAQEAHEAIRPTSAARDPESIRPYLEQDQYNLYKLIWNRFIASQMVPA
                     ILDVTRVESTPLETSARYVFRSSGTIVKFPGHTIVYMEGVDKELFAQKPKQEEEVEET
                     AERQLPELTEGERLRLVEQEEQTIPGVTSKQH"
     gene            complement(1534..2667)
                     /gene="dprA"
                     /locus_tag="VFH05_11165"
     CDS             complement(1534..2667)
                     /gene="dprA"
                     /locus_tag="VFH05_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET6678822.1"
                     /translation="MQSLAASKTGSLEAWMRLRTLEGIGDHTLLALVREWHSPDAVLA
                     APADELVQRGCSPRLAEVIRRGPDRDACRRIARELDAIARKRITVRTILDPAYPSHLR
                     TIADPPPLLYVSGSLEESDQLAVAVVGARRATPAGQMITERLANELAAAGFTIVSGLA
                     RGVDAVAHRGALTAGGRTVAVLGCGLGRTYPAEHERLRHEIEERGAVVSELPLDAPPH
                     SGHFPRRNRIISGLSLGVIVTEAAIDSGSLITARLAAEQGREVFAVPGFVRAETSRGT
                     HALIKEGAALIEEGQDVIDAIAPQLEPSTRARLVTPRKHTVAEDLGNHERLVYDALSY
                     DPLTVDHLYERTGLPVAAVMASLLSLELQRRVRQLPGQRYLKT"
     gene            complement(2728..4236)
                     /locus_tag="VFH05_11170"
     CDS             complement(2728..4236)
                     /locus_tag="VFH05_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET6678823.1"
                     /translation="MGVEIAITVAREETRVAVLDNGVVTDLFGDRAKYKDFVGNIYKG
                     KVAKVLPGMQAAFVDIGLEKAAFMHVSDLSLDAEPSDTLLDSDEDDKDGDDMRPRRES
                     SKPIEQLLTEGQELMVQISKGPIGTKGPRVTTYVSLPGRYLVFMPNVEHIGVSRRIAR
                     DEERARLKDIMKRVRNPGCGYIVRTVSEGVKEEELRSDVDFLHVLWQDIKTKRDQLAA
                     PALLHTDLSLSFRVVRDLFGKKVERLWIDSRQEYDAVRDFVQRFSPEQTSRIHFYDKD
                     ESLFDHLGVEQEMARALSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETIL
                     RNNLEAAKEVAYQVKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMASDKARTRISR
                     ISDLGLIEISRERVREDLLRSLSEPCRYCEGRGYTKSPTTVAYEIFREIRKIGSAAEQ
                     QRIVVGAHPSVAGLLQDEERQGLEALERECVAKVLVMPDEQLHLEQYDLVVM"
     gene            complement(4215..>4411)
                     /locus_tag="VFH05_11175"
     CDS             complement(4215..>4411)
                     /locus_tag="VFH05_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678824.1"
                     /translation="TAAIEFVLLNGWRGVVTTRLCYMCADREIGGKECIGTWGSPRST
                     SRRFDGAAERKESVWVWKLR"
BASE COUNT          845 a         1394 c         1195 g          977 t
ORIGIN      
        1 agtgctgctt tgacgtcact ccgggaatgg tctgctcctc ttgttcgacc agacgcagac
       61 gttcgccctc cgttaactca ggaagttggc gctccgctgt ctcctcaacc tcttcctcct
      121 gcttgggctt ctgtgcgaac agttctttgt cgactccctc catatagaca atcgtatggc
      181 cgggaaactt cacgatggtc ccgctggacc ggaagacata gcgggcggat gtctcgaggg
      241 gggtcgattc gacgcgtgtg acgtccaaga tggcggggac catctgagaa gcaatgaagc
      301 ggttccaaat taacttgtac agattgtatt gatcctgttc cagatagggc cggatggatt
      361 ccggatcacg cgcggccgag gtcggcctga ttgcttcatg agcttcctgt gccgccttgg
      421 aagtcttata gacattcggc acatcgggaa ggtattcggc gccgaaccgt tcctgaatca
      481 cctgccgggc gtcagccgtc gcttcctgag aaattctcgg cgagtccgtt cgcatataag
      541 tgatgagacc ggtggaacct tcgctgccga tctctatccc ttcgtagagt tgctgcgcaa
      601 gggtcatcgt cttcttgggc gtgaagtgca gtttcctggc ggcctcctgt tgcaaccggc
      661 ttgtgatgaa cggggcaacg ggattccgtt tcttttcctt ccgctcgatg gacgtgaccg
      721 caaacgcttt gccctcgatc gccgatacga tttgaccggc ctgttcggca tttccgatcg
      781 acgcctcttc tccatttata ctatggagct tggcctcgaa tggcggcggg ttggctccgg
      841 cgagcagcac aatgatcgac caatactctt ccgcacgaaa tgcttcgcgc tcggcctccc
      901 gttcgcacat cagtcggaca gcgacggatt ggacccggcc cattgagaga ccgcgacgga
      961 ccttattcca cagcaactgg ctgccttggt accccacaat ccgatcgagc acacggcgag
     1021 cctgttgtgc gttcaccagc ttcatgtcga tctggccggg agactgcagg gcctgcttga
     1081 ttgcccgctc cgtaatctcg ttgaaaagaa cccgaaagac tttcccgtcg ctctttttct
     1141 tggacttgcc gcgcagctct tccgagagat gccaggcaat ggcttcccct tcgcgatccg
     1201 ggtcaggcgc caaaaagatc ttgtcggact cctcagcctt cttcttgata tcagccagaa
     1261 ctttggactt tcccttgatc gtgacgtact ggggcttaaa atcgtgttcg agatcgacac
     1321 cgagtttgct ggtcggcaag tctttgatgt gcccaaccga cgccatgacg gtgtagcctc
     1381 gccccagata cttgctaatc gttttcgcct tcgtgggaga ctctacgata accagcgatt
     1441 tcgccatgtg gactccagat tcacaaccgt tcccctatca tccgtaccaa atatagcaga
     1501 tgagtgcaac caggccgccg gacaatggac ggtttaagtc ttgagatacc gctgtccagg
     1561 tagttgacgc acgcgccgtt ggagctcaag tgacaggagc gatgccatca cggcggccac
     1621 gggcaacccg gtccgctcat agagatggtc caccgtcagc ggatcgtacg agagcgcatc
     1681 atacaccagc cgttcatgat ttcccaaatc ttcggccact gtgtgcttgc gaggcgtgac
     1741 cagtcgggcg cgcgtggacg gttcgagctg cggcgcaatg gcgtcaatca catcctgccc
     1801 ctcctcgatc aatgcggcgc cttccttgat caaggcatgt gtgcctcgac tcgtttcggc
     1861 tctcacgaat cctggaaccg cgaacacttc gcgtccctgt tccgccgcga ggcgtgcggt
     1921 aatcaacgat ccgctgtcga tggccgcttc cgtgacaata acgccaagcg acagcccgct
     1981 gatgatacga ttgcgcctcg gaaaatgtcc gctatgcggc ggagcgtcga gcggtaattc
     2041 cgaaacgacg gctcctcgct cctcgatctc atgacggagc cgctcgtgtt ctgcgggata
     2101 ggttctcccg agtccgcacc ccagtacggc gacggtgcgg ccgccggccg taagggcacc
     2161 gcgatgcgca acggcatcaa ctccgcgcgc caagccgctg acgatggtga agcctgcggc
     2221 tgccaactca tttgccaaac gctcggtgat catctgccca gccggcgtcg cgcgccgcgc
     2281 gccaacgacg gcaacggcca gctggtcaga ctcctcaagc gagcccgaga cgtagaggag
     2341 cggaggcgga tcagcaatcg tccgcaggtg cgacggatag gcaggatcca gaatcgtccg
     2401 cacggtgatg cgcttccgcg caatcgcatc aagttcccgt gcgatgcgcc tgcacgcatc
     2461 acggtccggt cctcgcctga tgacctcggc caatcgtggg ctgcaacccc tctgcaccag
     2521 ctcatccgcc ggtgcagcga gaaccgcatc cggactgtgc cactcccgca ccagagcgag
     2581 cagagtatga tcgcctattc cttcgagcgt gcgaagcctc atccaggctt cgagggaacc
     2641 ggtttttgaa gccgcgagag attgcacgta gcctcacgtg tcctgggact caaccgccgg
     2701 acacgtaccc ggccgtggag ttcgctgcta catgaccaca agatcgtact gttctaaatg
     2761 caattgttca tcgggcatga caaggacttt cgcaacgcat tcccgctcga gcgcttccag
     2821 gccctgccgt tcttcgtcct ggagcagacc tgccaccgag ggatgcgccc cgacgacaat
     2881 tcgctgttgc tcggcggcac tgccaatctt tcgaatctcg cggaagattt cgtaggccac
     2941 cgtggtcggt gacttcgtat agccgcggcc ttcgcaatac cggcacggtt ctgagagcga
     3001 gcgcagcaga tcctcccgca cacgttcacg cgagatttcg atcaatccca ggtcggaaat
     3061 gcgggagatc cgagtgcgag ctttgtcgga tgccatggca tccacgaggg catggtacac
     3121 cttgtcccgg ttcttttccc gctccatatc gatgaagtcg acgatgatga tcccgccgat
     3181 tccacgcagt ttcacctgat aggccacttc cttcgccgct tccaaattgt tgcggagaat
     3241 cgtttcctct tgatcgcgtt tgccgacgaa gcggcccgtg ttcacgtcga tcaccgtcat
     3301 ggcttccgtg tgatcgatca ccaggtatcc gcctgatttc agccacacct ttcgactcag
     3361 tgccctcgcc atttcctgct cgacacccag gtgatcgaag aggctctcgt ccttgtcgta
     3421 aaaatggatg cgcgaggtct gctcgggaga aaaccgctgg acgaaatcgc ggacggcgtc
     3481 gtactcctgg cgtgaatcga tccacaagcg ctcgaccttt ttgccgaata aatcacggac
     3541 gacccggaag ctcaggctca gatccgtatg caaaagcgcc ggcgccgcca gttgatcgcg
     3601 cttcgtcttg atgtcttgcc agaggacgtg caggaaatcg acgtccgatc gtagttcttc
     3661 ctccttcacc ccctcgctga ccgttcggac gatgtaccca cagccgggat tgcggacccg
     3721 cttcatgatg tccttcaagc gcgcacgctc ctcgtcccga gcgattctgc gggaaacacc
     3781 gatatgttcc acgttcggca tgaacaccaa gtagcgtccc ggcaaggaca cataggtcgt
     3841 cacacgtggg cccttggtcc cgatcggccc cttcgagatt tgcaccatca gctcctgccc
     3901 ttctgtgagc aattgctcga tgggcttgct gctttcccga cgggggcgca tgtcgtcccc
     3961 gtctttatcg tcttcatccg aatccaacag cgtatcgctg ggttctgcat ccagggacaa
     4021 gtcggacacg tgcatgaacg cggccttctc cagaccaatg tccacgaagg ccgcctgcat
     4081 gcctggcagg accttggcga cctttccttt atagatgttg ccgacgaagt ctttgtactt
     4141 tgcccggtcc ccgaacagat cggtgaccac gccgttgtcc aataccgcca cacgagtttc
     4201 ctcccgtgcg acggttatcg caatttccac acccatactg actccttccg ttcagcggct
     4261 ccatcgaagc ggcgcgaggt agaacgcggg ctgccccagg ttccgatgca ctccttcccg
     4321 ccgatctcac ggtccgcaca catgtagcac aggcgggtcg tcaccacgcc ccgccatccg
     4381 ttcaataaaa caaactcaat cgccgccgtt t
//
LOCUS       DASSCQ010000160         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_394670, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000160 DASSCQ010000000
VERSION     DASSCQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394670"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1319)
                     /locus_tag="VFH05_11180"
     CDS             complement(3..1319)
                     /locus_tag="VFH05_11180"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET6678825.1"
                     /translation="MSEQSGEADRSQDTPEASPDTQSIPEYVEELVTRAREAANRLAT
                     LSTSVKNQALLAMADALEAQSERILDANGQDVDAFATRDKSAMADRLRLTEARIRDMA
                     DGIREVAKLPDPLGEMAKMWTRPNGMQVGRVRVPIGVIGIIYESRPNVTADSAALCLK
                     SGNACVLRGGSEAIKSNSAIAAILSEAAEKCGIPAGAISFVARPDRELVPLLLKKDRS
                     IDLIIPRGGESLMRIVAEHSTIPVIKHDAGVCHIYVDDDADPAMAERVCLNAKVQRPS
                     TCNAMETLLVHQSLARTWLTGFIDKLVAAGVEVRGCQKTCALSSAAKPASDSDYGKEF
                     LDLTLAIKVVKHIDEAMEHIVRYGSRHTEAIVTSDYKKAMRFLREVDASAVLVNASTR
                     LNDGYQFGLGAEIGISTSRIHARGPMGLEELTCSKFIVLGSGQVRE"
     gene            complement(1425..2435)
                     /locus_tag="VFH05_11185"
     CDS             complement(1425..2435)
                     /locus_tag="VFH05_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET6678826.1"
                     /translation="MRIVITGGAGFLGSHLCDSLIEAGHSVICMDNLLTGRMENISHL
                     LGHERFVFLRYNVCDYLHVDGPLDAIMHFASPASPQDYMEFPIATLKVGALGTHKVLG
                     LARAKGARFLLASTSEVYGDPLVNPQPEAYWGNVNPISPRGVYDEAKRFAEAMTMAYH
                     RYHGLDTRIVRIFNTFGPRMRANDGRVVSNFIVQALQGKPLSVFGEGQQTRSFCYVDD
                     LVRGIVGLLLVNSDKTIEERTDKKFLYARAGDRVVASVHDPVNIGNPRELTVIEIARL
                     IVKLTGSSSDISYHPLPTDDPKVRRPDISRAHALLKWEPQVPLEDALSRTIAYFRKAL
                     SQ"
     gene            complement(2515..3081)
                     /locus_tag="VFH05_11190"
     CDS             complement(2515..3081)
                     /locus_tag="VFH05_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET6678827.1"
                     /translation="MPDTIFPRLQSQNGVTYLMLMFAIVLIGLSMTVAAKQWKTMVQR
                     ELEADLMAKGIEIQNALALYSATMKAGRVTQTEVYPQSLADLTRLPKPFLRKAYTDPM
                     GHRDWEYLRGPTGGIMGVRSSSRAKPLKKTDFPQVVRHFEGRASYHDWVFQHPNPSSS
                     GSFPFSPVQAGTLVSPPPVAPQPILPPS"
     gene            complement(3088..>3449)
                     /locus_tag="VFH05_11195"
     CDS             complement(3088..>3449)
                     /locus_tag="VFH05_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678828.1"
                     /translation="REMVFATPRNIFALPSASVANQPAPMEASDRALRQQSIAAELSQ
                     FHYVGFVRTGEQWEKKQDMAVLTKKDDLHIVRKGETVEAHVVVKKITQESVTLLDKDS
                     RLEYTVLLSEEPQTPSP"
BASE COUNT          722 a         1008 c          953 g          766 t
ORIGIN      
        1 cactactcac ggacctgccc gcttcccaac acgataaact tggaacaggt cagctcttcc
       61 aatcccatgg gtccgcgcgc atggatgcga gaggtactga tgccgatttc tgcacccagc
      121 ccgaactggt atccatcgtt gagtctggtc gacgcattga ccagcacggc gcttgcatcc
      181 acctcgcgca ggaatcgcat cgccttctta tagtcggacg tgacgatggc ctccgtgtga
      241 cgagacccgt agcgaacaat gtgctccatg gcttcatcga tatgcttcac gaccttgatt
      301 gccagcgtca aatccaaaaa ctccttccca tagtcactgt cactcgcagg cttggctgcg
      361 gacgataagg cgcaggtctt ctggcagccg cgaacttcca cgcctgctgc aaccagtttg
      421 tcgataaaac ccgtcagcca ggttctcgcc agactttgat gaaccaacag cgtctccatg
      481 gcgttgcacg tcgagggccg ctgaaccttg gcattgagac atacacgctc ggccatggcc
      541 ggatcggcat cgtcgtcgac atagatatgg cacacgcctg catcgtgctt gatgacaggg
      601 atcgtcgagt gttcggcgac gatccgcatg agggattctc cccctcgtgg aataatcaga
      661 tcgatcgacc ggtctttctt caagagcagc ggtaccagct cccggtccgg cctggccaca
      721 aacgagatcg caccggccgg aatcccgcac ttctctgcag cctccgagag aatcgcggcg
      781 atggcactgt tcgacttgat cgcctcactt ccacctcgta acacgcacgc gttgccggac
      841 ttcaggcaca atgccgccga atccgccgtg acattggggc gcgactcata aatgatgccg
      901 atcacgccaa tgggcacgcg aacgcgtcca acctgcatgc cgttgggcct ggtccacatc
      961 ttcgccattt ccccgagcgg atcgggtaat ttggccacct ctcggatacc atcagccatg
     1021 tcccggattc gtgcctcggt gagacgcaga cgatcagcca ttgccgactt gtcccgcgtg
     1081 gcaaaggcat cgacgtcttg cccgttcgcg tccaggatac gttccgattg tgcttccaac
     1141 gcatcggcca tagccagtaa cgcctgattc ttgacagatg tcgacaaggt cgccaatcga
     1201 ttggccgcct cgcgcgcacg cgtgacgagt tcttcgacat actcagggat ggattgcgta
     1261 tctggggacg cttctggagt gtcttgcgaa cgatcggcct cgccggactg ctcagacatg
     1321 aagtggtgct cctcttgtgg tcgctggggc tctacaggat acagtgggga tttccgaagg
     1381 gtcaaggcat cccgggagac gcctcgcaac aaaacagatg gtgattattg agagagtgct
     1441 tttcgaaagt acgcgatggt acgactcaac gcatcctcga gggggacctg cggctcccat
     1501 ttgagcaatg catgcgctct gcttatatcg ggccgacgga ccttgggatc gtcggtaggc
     1561 agcggatgat agctgatgtc gctggaggag ccggtcagct tgacgatcag tctggcgatc
     1621 tcaatgaccg tcaactctcg cggattgcca atgttgacag gatcatgcac gctggccacc
     1681 actcgatcgc cggctcgggc gtacaggaac ttcttatccg tccgctcctc gattgtctta
     1741 tcggagttga ccagtaatag accgacgatg cctcggacca ggtcgtcaac gtagcagaag
     1801 ctcctggtct gctggccctc gccgaacaca ctcagcggct ttccttgcaa ggcctgaacg
     1861 atgaagtttg acaccacccg cccgtcattt gcccgcatgc ggggaccaaa tgtattgaaa
     1921 attcgcacga tgcgcgtgtc gaggccatga tagcggtgat aggccatcgt catcgcttcg
     1981 gcaaatcgct tggcttcgtc gtagacgcct cgcgggctga tcgggttgac gttcccccaa
     2041 taggcttccg gctgcggatt caccagcgga tccccataga cctcggaggt gctggccaga
     2101 agaaatcggg cgccctttgc gcgcgccaat ccgaggactt tatgcgtccc gagcgcaccg
     2161 accttgaggg tcgcaatcgg aaactccata tagtcttgcg gactggcagg cgaggcgaag
     2221 tgcatgatcg cgtcgagcgg gccatccaca tgcagatagt cgcaaacgtt gtacctgaga
     2281 aacacgaacc gctcatgccc taacagatgg gaaatgtttt ccatccgacc cgtcaacaga
     2341 ttgtccatgc agatgaccga gtgcccggct tcgatcagcg agtcacagag atggctgcct
     2401 aaaaaccctg ccccgcccgt aatcacaata cgcatgtttg cactccttca tgagccgtta
     2461 caccacgact gtagcagttt tgcttcataa atcccaacgt gttcttcata tgagttatga
     2521 ggggggcagt atcggctgcg gagcaaccgg cggaggacta actagtgtcc ccgcttggac
     2581 cggcgagaag gggaacgatc ctgagcttga cggattagga tgctggaaca cccagtcatg
     2641 gtaagaggct cgtccttcaa aatgtctcac cacctgagga aaatcggttt ttttaagagg
     2701 cttcgcacgg ctgctgcttc ggacgcccat gatgccgccg gtcggaccac gcaagtactc
     2761 ccaatctcga tgccccatag gatccgtata ggccttgcga aggaatggct ttggcagcct
     2821 cgttaaatcg gccagggatt ggggatatac ttccgtctgc gtgacccggc cggctttcat
     2881 agttgcggag taaagtgcca gcgcattctg gatttcgatg cctttcgcca tgaggtcggc
     2941 ttccagttcc cgttgcacca tggtcttcca ttgcttcgct gcgacggtca tcgacaggcc
     3001 gatcaacacg atggcaaaca tcagcatcag gtaggtgaca ccattttggc tctgaaggcg
     3061 gggaaagatg gtgtcgggca tggcgactca gggggatggt gtttgaggct cttcggagag
     3121 cagcacggta tattccaagc gtgaatcttt atcaagcagg gtgacacttt cctgagtaat
     3181 cttttttacg acgacgtgag cttcgacagt ctcacctttc ctcacgatgt gcaaatcgtc
     3241 cttctttgtc agcacggcca tgtcctgctt tttctcccat tgctctcctg tccgaacgaa
     3301 ccccacgtag tggaattgtg acagctcggc ggcgatggat tgctggcgta aggcgcggtc
     3361 agacgcctcc atcggtgctg gctgattcgc caccgacgcg gagggcaaag caaaaatgtt
     3421 cctgggggtg gcaaacacca tttctcgct
//
LOCUS       DASSCQ010000161         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1293064, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000161 DASSCQ010000000
VERSION     DASSCQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293064"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1083
                     /locus_tag="VFH05_11200"
     CDS             259..1083
                     /locus_tag="VFH05_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678829.1"
                     /translation="MAARWNVIVMSVLFLLQGMHASAMGGTTSASAEGQKLGESKEQV
                     VTEPTQNQLPGNRLVTGRILSIRSNQMEIDIGNVKSLYVPLRPAQVKGQTFEVGQPII
                     VTLNDHNAVVDYHHPNERSDHQVLHGRLTTPLTVGLDKAVIETKDGTKTLMVTDRAKG
                     KLAAIPVGVDAYFLADESGQLVDAQLASSEAVRESGQNNKARIKGAHEQVRATFKGQG
                     GEGKLKIAEAGKEREVPFRPPLEKLDRLKPGQDVVLLMDDQGYVLEIATPEVTPAR"
     gene            complement(1187..1750)
                     /locus_tag="VFH05_11205"
     CDS             complement(1187..1750)
                     /locus_tag="VFH05_11205"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET6678830.1"
                     /translation="MTRLTGLMTAVLAHGLFYGCAFIQQSLPGLAMTDAEMVGVLHSF
                     SEEEVEAAELARRTSTTSDVQAFAGRVLNEHRQLAQQNERLARQLHITPREPALGSEL
                     KATHQQAMHNLEGKVGSDFDRAYVEYEIARHVQAFGLMETAAESEATLPLQQELIRTG
                     PDLLSHLSAARALERRLVAKDDQSESP"
     gene            complement(1767..2357)
                     /locus_tag="VFH05_11210"
     CDS             complement(1767..2357)
                     /locus_tag="VFH05_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678831.1"
                     /translation="MLLRHYLLFFILPLWVAAGLTDYVLHKRAQIEDNAGTKESILHA
                     LQLGEAGIPVVLALLLEINALIFLVMVLAFILHELTSLWDVSYASAHRDVSPLEQHVH
                     SFMEVLPLMALSFVTILYWNQFVALIGLGPEPPRFELRPKSNPLSTGYLVGLFLCIML
                     FVVLPYGEELWRCLRASRSRHIRDNTKQAFRPSQAA"
     gene            2664..>2846
                     /locus_tag="VFH05_11215"
     CDS             2664..>2846
                     /locus_tag="VFH05_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678832.1"
                     /translation="MARKGGKRYRYRTNKQDIDWQEERADASQTIGEMQRLFSAIAGV
                     GLILGGLRRRSLGGGAL"
BASE COUNT          647 a          737 c          867 g          595 t
ORIGIN      
        1 cgggctatcc catgggctgt cgagcctacc gagcgtcaaa tcacgtttag cagggagtga
       61 agggattcgc agcttcgtct caatttctcg ggtagttctt agacagcctg tctgaatgac
      121 tcgggtgctg cctagtcgct cgactattcg aaatcccgag accggccgtt ttccggtccg
      181 caggaatccg ccgattggtc caacacttgc tcgcaataat tgtattgaga atccatgaca
      241 caacaagggg ggtgtgcaat ggctgcaagg tggaacgtca tcgtgatgag tgtgctcttc
      301 ctgctgcaag gcatgcatgc atctgcaatg ggcggtacta ccagcgcgtc ggcggagggg
      361 caaaagcttg gggaaagcaa agagcaggtt gttactgaac ccacgcagaa ccagttgcct
      421 ggcaatcgcc tggtcaccgg ccgcattctg tccattcgca gtaaccaaat ggaaatcgat
      481 atcgggaacg tcaagtcgct gtacgtgccg ctccggcctg ctcaggtgaa gggccaaacg
      541 tttgaggtcg ggcaaccgat tatcgtcaca ctcaacgatc acaatgccgt ggtcgattat
      601 catcatccca atgaacgatc cgaccatcaa gtattgcacg gtcgtttgac cacgccgctg
      661 acagtgggat tggacaaggc ggtgatcgaa accaaagatg gaaccaagac gttgatggtg
      721 accgatcgtg ccaaaggaaa gctcgcggcc attccggtgg gggtcgacgc gtactttttg
      781 gctgatgaga gcggccagtt agtagacgcc caactcgcca gctctgaggc ggtccgagaa
      841 tcagggcaga acaataaagc ccgaattaag ggcgcgcatg agcaagtgag ggccactttc
      901 aaaggacagg gcggcgaagg caagttaaaa attgccgaag cgggcaagga aagggaagtc
      961 ccgtttcgtc ctccgctgga aaagctggac cggctgaaac cggggcagga cgtcgtctta
     1021 ttaatggacg atcaagggta cgttctggag atcgctacgc cggaggtcac gcctgcgcgg
     1081 taggcaacac gtcgggggct ttaatgggaa catgagcatt acggcccctg acactccact
     1141 cgaatggcgt ggaaagggga gaacgctatc agggcgaccc ggcggcctag ggggattcag
     1201 attgatcatc tttcgcaacg aggcggcgtt ccaatgcccg cgccgcagac aaatggctga
     1261 gaagatcggg tcccgttcgg atgagttcct gttgcagcgg gagggtcgct tcagattcgg
     1321 cagccgtctc catcaggccg aaggcctgca cgtgtcgagc aatctcatat tccacgtagg
     1381 cgcggtcaaa gtcggatccc accttccctt cgaggttatg catcgcctgt tgatgggtgg
     1441 ctttcagctc ggagcccagg gccggctcac gcggggtgat gtgtaattgc cgggccagac
     1501 gttcattctg ttgtgctaat tgtcgatgct cgttgagcac gcgtccggca aacgcctgga
     1561 catcggaagt ggttgacgtg cgtcgcgcca gctcggctgc ctcgacctct tcctcagaga
     1621 aactgtgtag gacgccgacc atctcggcat cagtcatggc cagccccggt agggattgtt
     1681 gaataaaggc acacccatag aagagcccgt gcgccagtac ggccgtcatc aaaccggtaa
     1741 ggcgagtcat cggtgtctcc tataagttag gccgcctgtg acggccggaa cgcctgtttc
     1801 gtgttgtccc ggatgtgccg agagcgcgaa gcgcgcagac agcgccagag ttcttcaccg
     1861 tacggcagca ccacgaagag catgatgcac aagaacagtc cgacgagata tccggtcgaa
     1921 agtggattgg atttgggtcg cagttcaaat ctgggtggtt cagggcccag gccgatgagg
     1981 gccacaaact ggttccaata caggatcgtc acgaacgaca gtgccatcaa gggcagcact
     2041 tccatgaagc tgtgtacgtg ctgttctaat gggctcacgt cgcggtgtgc cgatgcgtaa
     2101 ctgacgtccc agagtgaggt caattcgtgc agaataaatg cgagcaccat gaccaggaag
     2161 atgagtgcgt tgatctccag caggagggcc agcacgacgg gaatgccggc ctcgcctaac
     2221 tgcaaggcgt ggaggatcga ttctttcgtc cctgcgttgt cttcgatctg agcgcgtttg
     2281 tgcaggacgt aatcagtgag acccgcggcc acccataagg gaaggataaa gaacaacaag
     2341 tagtgtctga gtagcatcgc gggatcccac atgccttcat gctagtccgt cggactctcg
     2401 ttgtggcctg gggaagatcc taaggcctac cgctgatgca ttgaggaccc cggtgcaagc
     2461 caatggaaaa gctgtccggc gtaagacagt ctgtctaaat ccatcatagt catccgacaa
     2521 ttcatgtcgt tcgttgcaga cctgttcggt ttttgaggct ctggtcaggc gtttctaaag
     2581 gtctgtgttt tgcaagacta tccgattgca aatgaagtca cgctactgcc cggtcaaccg
     2641 ttcgaatgtg gagggagtat ttcatggcac ggaaaggcgg caaacgatat cgctatcgga
     2701 cgaacaagca ggacattgat tggcaggaag agagagccga tgcctctcag accattggcg
     2761 agatgcagcg gttgttttcg gcaatcgccg gtgttggatt aatcctgggc gggttgcgcc
     2821 gtcgatcctt gggcggcggc gccctg
//
LOCUS       DASSCQ010000162        10549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_50342, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000162 DASSCQ010000000
VERSION     DASSCQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50342"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFH05_11220"
     CDS             <1..1017
                     /locus_tag="VFH05_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6678833.1"
                     /translation="ALLELVNQMAGKKDFSKVPNLIYRQNGKITVNQPFYSENVNQLP
                     APNYDGFPLDRYLSPEPVLPVQFSRGCYYKDCAFCALTLDHQNFRQKDPGRTIEELEW
                     LKQRYGVRNFFFTDECFALSPTKRLCQQMIERRVDVKWTCEMRFEKHLTRELLTSMRD
                     AGCLKIVFGLESFNQRIMDFMKKGIQQEWVRRISSDCVDLGIAVHCYIIVGFPTEREE
                     EALETMNFIVENKKLNESYGFSCQPCLFDLEKEAPIMSDPGGYGIRRIMRPSAEDLSL
                     GFFYEVQEGMTPDQAERLYQHVYEKVSEVVCELPFNYSMADGLLYIARAKAEERRAVT
                     AAQT"
     gene            1040..2155
                     /locus_tag="VFH05_11225"
     CDS             1040..2155
                     /locus_tag="VFH05_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678834.1"
                     /translation="MSATATTIERITGKLSDQPLLFQYTIKLVLLVAFLELILYRLVS
                     RLGMHLSKLAADHQWIIPTFTALTEVGQWLLNVVAILLFLGLTVGMVNRVAHRGFTGL
                     TRFVVPCIALLLLLTVGFLFVPPSMLGSAIYNTVALAAIVLLMSEYLATHHDRSHRVL
                     GLTYLLGISGWLYYQIVSTFFSFSGSVVAPPMVYESHRAGEAFMVLASILVFWAYGRG
                     VSFRSRNQRQRHRALWFWSTAATIFLTMFFLDYLLNRYNPALASNVRKAADGIGWIFQ
                     FGMGYTFYLPFALYMAGLLCWSYTVIKLLTMGRLAGYGLGLMFIAGYALLYSNLTLMV
                     VLGVMLLTMDRYRRDAADSIADRTVLRTTDSLVGGHV"
     gene            2178..2978
                     /locus_tag="VFH05_11230"
     CDS             2178..2978
                     /locus_tag="VFH05_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6678835.1"
                     /translation="MEETTSSEQTRAAVDPGDQPLNPDEEIAEIEKLLAAEPDDFQAR
                     CRLGELYFSKGRLDDALVEVKKSIEMAEGIRAEMNRSLAMYYSNLGTIYATKNMADEA
                     EAEFKRALDVFQYDVLALFNLGRLCADKKKYMDAKGYYERLVEITPDDPMAWYNLAGV
                     YVELDNPQVSDYNTIDAAIQCYLRTLELDPKHLEASFKLMELALNHKKSDLAIKVMED
                     AVEHNADEPLAYYNLISVYDKCKMFEQAEQARQRLKERFAKRSKTSAS"
     gene            3006..3275
                     /locus_tag="VFH05_11235"
     CDS             3006..3275
                     /locus_tag="VFH05_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6678836.1"
                     /translation="MFGSMGITELILILFIVLVIFGAGKLPQLGEGLGKAIKGFKKSV
                     HEADAIEAEAEAAAAQQQMASPQPSISTTPASGVEQPAAQSATRA"
     gene            3297..4550
                     /locus_tag="VFH05_11240"
     CDS             3297..4550
                     /locus_tag="VFH05_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678837.1"
                     /translation="MDTELQLAVGYIETFGGNGKARSTGDGKRAVKAGIPLPHHVALD
                     KNERWLYFAESGSDRIRRIDLQEGTLQNFAGIGETCYSGDEGPCGEAGLYLPLDVAFD
                     SQNHLYICDSGSNRIRKVDRDTGVITTIIGTGQHGFNGDGPALEVNLTWPAAMAFDKD
                     DVLYIADTQAHKIRRYDPRTGMVTTIAGTWTPEDDAREQPLVARNLVVLSGDAIGIDF
                     SDDHGWLMPVCSDGLDMSMYLDDGKPAMEARLYDVVGIAVDGKGDVVFVDKGSNRVRK
                     IDSATGVISTLAGVCRYGYDGDNKPATRAMLHAPEAVVFDAQDNLYVSDTMNHRVRRV
                     DAATGIITTVAGNGDSGYEDKNMGGCGAARFVAKESAGALKHGDGLLGVEAVVNSPVG
                     LALDSLGHLYICERGENKIRRVRLW"
     gene            4677..5507
                     /locus_tag="VFH05_11245"
     CDS             4677..5507
                     /locus_tag="VFH05_11245"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HET6678838.1"
                     /translation="MARKTTTSPSRSLLLLFFCILVSGAVLGGLGIPLVSSEGMVIRA
                     HLIEGDLPVTPEDAGWAKVAPMTLPLSGQVITRPVWPEPTARALTVRSVHNGTDIAFL
                     LEWQDNTKNDRLTPGTFRDGVAIGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDR
                     RASRSNDKQREGVRTFEVIPRRVSSVEDLIGGGFSTLTTKEKQGRVQGKATWKDGVWH
                     VVMRRPLGAEEQDNEAKLVPGRVQTVSFAVWNGENKERNGQKAVAPWFQLVIDPIAKI
                     "
     gene            5640..6056
                     /locus_tag="VFH05_11250"
     CDS             5640..6056
                     /locus_tag="VFH05_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6678839.1"
                     /translation="MCGVLLGLLGIPLPGRTANDRAADGMTVDEAAKLGEDFGVIVGD
                     VDEEIQKELKLQRPQGVAVFEVIGNSRADYAGIKVRSVIKEIDKKEVRNMLDFGRHIK
                     KAINECNFTVGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            6175..7500
                     /locus_tag="VFH05_11255"
     CDS             6175..7500
                     /locus_tag="VFH05_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET6678840.1"
                     /translation="MQFRRMLTKRRIAATCLVLLLFGVIAFYQDRAVAQKADGQALNG
                     QASDGWVGEIEQVFIRSEECKQCHDRHYEEWKGAREQTPDLKTFGRVDAALLHGTSLE
                     SPVFRTVLGVWLQTNPTAEERKRCLSCHVPATTVFPQHAEKITAQILSGTPQIEGIGC
                     ASCHLIKSVETTMGPPPTFKIEPGKTMHGPYADPEENLVHPAVQSPLFRGANYCASCH
                     FDKVKDVTQKDLPGEILQGTICQDCHMEQSTGSSTSKRGAMTRSIGRHYFRGVVIPGI
                     LLKNRNLQAEWMPRIDIDSTKSAGKVEGTALVKVGSLPHSFPDGDPVLKQFMLAIAVK
                     DARGKVLAQETKQFGLPYDKILRGPIPDPFIKGGNTRKVPFSLAVPGGASAASVEAVL
                     SYSLIPTPEPALKDKYLATLKDDKEREKAKKILDEYTQPRMLTYRAKTF"
     gene            7523..8770
                     /locus_tag="VFH05_11260"
     CDS             7523..8770
                     /locus_tag="VFH05_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET6678841.1"
                     /translation="MSVRKTIVPVALTAMFLVAGWLSAPLFGDRIFAQQDGKAPITMD
                     KAFPNSNKCKRCHERVFEEWETSPLSRSIHSPAFRASLDAFLSSPAGKDKALCFRCHA
                     PHVREFPDQVQLFIDQAKSGDPQMDGVACSQCHLIKQVDRTKQPPEPKYEPAGSKTLY
                     GPYKDFAQNLAHQSLELGLFRKSDLCLNCHQAVPSAANLGKSNDLLGNWDQSKAIKSG
                     KECQSCHMPEQTGESANGEKKRTVANHTFPGRIGKLRQEAAKLDIETKIEGEKTTVVL
                     KVQSLVPHNLPTTHPAWASVVLQLDIKGKNLKTVFSDKRVYGRVFQDAKGQKTVFDFE
                     AVKVLEDTVLKPEELRVETFTFPTPKDTRTFDVEATLSYAPVSGPASFLQRIEAESSK
                     GSSDPVFQPIDIVKKTENIPVNK"
     gene            8898..10250
                     /locus_tag="VFH05_11265"
     CDS             8898..10250
                     /locus_tag="VFH05_11265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6678842.1"
                     /translation="MRLSKTSVIVVGAGLAGLSAAVRLMKDGAHVTVLEARDRVGGRV
                     LTVRDAFARGQHAEAGGDFIDDSQEDIRRLADQYGLTVRTILRQGFSFVRDKGKGQIH
                     GRPLKGERAWKPIVDRAKPLIEAYRRAEQQWESAVVRSIAQRSVAQWLDEIDADGNMR
                     AMVRGLRGFFLADPEDLSLLVLIEQLASEAPGQEGMGRIEGGNDRLPRVMAVELGESL
                     HLNTAARAIHDDQPFVHVTVESAGGDRTQIKADYVILSVPATALRAIEITPDLPLEQA
                     KVFERLKYGRVTKSLLQFDRRFWKRKGRSPAYGTDAPTGAIWDANEEQRGSAGILTLM
                     AGGQASEDTQKILAQEGVGGLVRALEWLKPGDAKLLHSHHVTWEHDSWSQGGYAYFDP
                     SFDPAWRPWLARPHGRLLFAGEHTSIKWQGYMNGAVESGLRAAAEVWALAAAGNRRAP
                     "
     gene            complement(10257..>10549)
                     /locus_tag="VFH05_11270"
     CDS             complement(10257..>10549)
                     /locus_tag="VFH05_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678843.1"
                     /translation="EDFVIVRAGETGMGLEPNYIVVDGQTFSKTKLALDNHVYKNCNI
                     DDCDLYYSGGQYELLDTHITNSRLILNHPAKGIYNALQIFKMKSPGSRIVYE"
BASE COUNT         2216 a         2977 c         3276 g         2080 t
ORIGIN      
        1 gccctactcg agctcgtcaa ccagatggcc gggaaaaagg attttagcaa ggtgccgaat
       61 ctgatttatc gtcagaacgg caagatcacc gtcaaccagc cattctattc cgagaacgtc
      121 aaccagttgc cggcacccaa ttacgacggc tttccgctgg accgctatct ctcccccgag
      181 ccggtcctgc cggtgcagtt ctcccggggt tgctactaca aggactgcgc cttttgcgcc
      241 ttgacgctcg atcatcaaaa cttccggcaa aaagatccgg gccggacgat cgaggagctg
      301 gaatggctca agcagcgtta cggcgtgcgg aacttctttt ttacggatga atgcttcgcc
      361 ctgtcgccga cgaaacgcct ctgtcagcag atgatcgagc gccgggtcga cgtgaaatgg
      421 acctgcgaga tgcgtttcga aaagcatctc acgcgcgagt tgctgacgtc gatgcgagac
      481 gccgggtgtt taaaaatcgt cttcggcctc gaatcgttca atcagcggat catggatttc
      541 atgaagaaag gcattcagca ggagtgggtg cgacgtatct cgagcgactg cgtggatctc
      601 gggatcgccg tccactgcta catcattgtc ggcttcccga ccgaaaggga agaggaagcg
      661 ctggagacga tgaacttcat cgtcgagaat aaaaagctga acgagtccta cggcttctct
      721 tgccagccct gcctgttcga tcttgaaaag gaagcgccga ttatgagcga tccgggaggc
      781 tacggcatca gacggatcat gcggccctcg gctgaagatt tgagccttgg atttttctat
      841 gaagtgcagg aggggatgac gccagaccaa gccgagcggc tctatcagca tgtgtacgaa
      901 aaagtcagtg aggtggtgtg cgagcttccc ttcaattact cgatggcgga tgggctcctc
      961 tacatcgcac gggccaaagc ggaagaacgg cgcgcggtga ccgccgcgca gacataaggt
     1021 gagaagacag gagataggcg tgtccgcaac tgcaaccacc atcgagcgca tcaccggaaa
     1081 gctgagcgac cagccgctgc tgttccaata cacgatcaag ctcgtgttgc tggtcgcgtt
     1141 tctggaactg attctgtacc ggctggtctc ccggctgggc atgcacttga gcaagctggc
     1201 tgccgatcat cagtggatca ttccaacctt tacggccctc acggaagtcg gccagtggct
     1261 cttgaatgtg gtcgcgattc tcttgttcct cggcttgacg gtgggcatgg tcaaccgcgt
     1321 ggcccaccgg ggctttacgg gcctgaccag gttcgtcgtg ccctgtatcg ccttactgtt
     1381 gctgctgacc gtcggctttc tattcgtgcc gccctcaatg ctcgggtcgg cgatctacaa
     1441 taccgtcgcc ctggcggcca tcgtattgct gatgagcgag tacctggcga cacatcacga
     1501 tcgttcgcac cgtgtgctcg gcctcacata cctcttgggc atttccggct ggctgtacta
     1561 ccaaatcgtc tcgacgttct tcagtttctc agggagcgtg gtggcgcccc caatggtata
     1621 cgaatcacac cgcgccggtg aagcgttcat ggtgctggcg agcattcttg tgttttgggc
     1681 ctatgggaga ggcgtgtcgt tccggtcgcg caaccagcgt cagcgacacc gggccctctg
     1741 gttctggagc acggcagcga ccatttttct gacgatgttc ttcctggatt atctgctcaa
     1801 ccggtacaac ccggcgctgg cgagcaacgt gcgcaaggcc gccgacggca tcggatggat
     1861 tttccagttc gggatgggct acacctttta tctcccgttc gcgctctata tggccggatt
     1921 gctctgctgg tcctatacgg tgatcaagtt gttgaccatg ggccggctcg ccggctacgg
     1981 tttggggctg atgttcatcg ccgggtatgc gctgctgtat tccaatctga cgctgatggt
     2041 cgtgctgggc gtgatgctgt tgacgatgga tcgctaccga cgtgacgccg cggactcgat
     2101 cgcggatcgg acggtactac gaacgaccga ctcgttggtc ggcggacatg tgtaatcttt
     2161 tgccataggg gcttgccatg gaagaaacga cgtcttcgga gcagactcgg gcggcggtgg
     2221 atccgggcga tcagccgttg aacccggatg aggagattgc cgaaattgaa aagctcttgg
     2281 ccgccgagcc ggatgatttt caagcccggt gccggctggg tgaactctat ttcagcaagg
     2341 gtcggttgga cgacgccttg gtcgaggtca agaaatcgat cgagatggcc gagggcattc
     2401 gcgcggaaat gaatcggtcg ctggcgatgt actattcgaa tctcggcacg atttacgcga
     2461 cgaagaacat ggctgatgaa gcggaagccg agtttaagcg tgccctggac gtgtttcagt
     2521 acgacgtcct ggcgctgttc aatttggggc gtctctgtgc cgacaagaaa aagtacatgg
     2581 atgcgaaggg gtattatgag cggctcgtcg aaatcacgcc ggacgatccg atggcctggt
     2641 acaatctcgc cggcgtctac gtggaactcg acaacccgca ggtgtcggac tacaacacga
     2701 tcgacgccgc gattcaatgc tatctccgga cgctcgaatt ggaccccaag catctggaag
     2761 cgagcttcaa gctgatggag ctggctctca atcacaagaa atccgatctc gccatcaaag
     2821 tgatggaaga cgccgtcgaa cacaatgcgg acgagccgct ggcctactac aacctgatca
     2881 gcgtctacga caagtgcaag atgttcgagc aggccgagca ggcccggcag cggttgaaag
     2941 aacgatttgc gaagcggagt aaaacgagcg catcctgatt cacccttatc gcggaggaac
     3001 acaccatgtt tgggagtatg ggtatcacag agctgattct gatcctcttc atcgtgctgg
     3061 ttatttttgg cgccggcaaa ctgccgcaat tgggagaagg actcggaaaa gccatcaagg
     3121 gattcaagaa gtcggtgcat gaagcggatg ccatcgaagc cgaagctgag gccgcagcgg
     3181 cccagcagca aatggcgtcg cctcagccgt ccatttcgac gacgccggcc tcaggcgtgg
     3241 aacaaccggc cgcgcaatcc gcgactcgcg catagcgcga cgatcggccg aacgccatgg
     3301 acacagaact tcagctcgcc gtcggctaca tcgagacctt cggaggcaac ggtaaagcgc
     3361 gcagcaccgg cgacgggaag cgggccgtca aggccggtat tccgttgccg catcatgtgg
     3421 cgcttgacaa gaacgagcgc tggctgtatt tcgccgagtc cgggtccgac cggatccggc
     3481 gcatcgatct ccaggaaggc acgctgcaga acttcgccgg catcggcgaa acctgttaca
     3541 gcggcgacga gggtccttgc ggcgaggcgg gtttgtacct gccgctggac gttgccttcg
     3601 attcccagaa tcatctgtac atctgtgact cgggcagcaa ccgcatccgc aaagtcgatc
     3661 gcgacacggg cgtgattacc acgatcatcg gcaccggcca gcacggattc aacggggacg
     3721 gaccggcgct cgaggtcaat ctgacctggc ccgcggcgat ggcgttcgac aaagacgacg
     3781 tgctctacat tgccgatacg caagcgcata aaatccgccg ctacgatccg cgtaccggca
     3841 tggtgacgac gatcgccggc acctggaccc cggaggacga tgcgagggag cagccgctcg
     3901 tggcccgtaa tctcgtggtg ctctcgggtg atgcgatcgg catcgatttc agcgacgatc
     3961 acggctggct catgccggtc tgctcggacg gtttggacat gtcaatgtat ttggacgacg
     4021 gcaagccggc gatggaagcc cgcctctacg acgtggtggg catcgcggtc gacggcaaag
     4081 gcgacgtcgt gttcgtggac aagggcagca accgtgtaag aaagattgac agcgccaccg
     4141 gcgtgatttc cacactggcc ggcgtctgcc gctacggcta tgacggcgac aacaagccgg
     4201 ccacacgtgc gatgctgcat gcgcctgagg ccgtggtctt cgacgcgcag gataatctct
     4261 acgtctccga cacgatgaat caccgggtgc gtcgagtgga tgcggcgacc gggatcatta
     4321 ccaccgtcgc aggcaacggc gacagtggct acgaagacaa gaacatgggc ggctgcgggg
     4381 cagcgcggtt cgtcgcaaag gaatctgccg gtgcgctcaa acatggcgac ggcctgctcg
     4441 gcgtcgaggc cgtcgtcaat tcgccggtgg gactagcctt ggattcgctc ggccatctct
     4501 acatctgcga gcgcggcgag aacaagattc gccgcgtccg tctttggtag aaacccgaat
     4561 ccgatcgaat tggtcgctcg aatctgttgt ggtatggtga cacggtcccc gcggcatcac
     4621 ggtccaggga cggtgtcgga gagtctgctg tcggcttcgt taaccaggac gtgagcatgg
     4681 cgcgcaagac gaccacgagt ccttcccgct ctctcctgct cctctttttc tgcatactcg
     4741 tttcgggcgc ggtcttgggc ggcctcggta ttccccttgt cagctcagaa ggcatggtca
     4801 ttcgtgccca tttgatcgag ggcgatctcc ccgtgacccc agaagacgcc ggctgggcta
     4861 aggtggcccc catgacgctt ccgctcagtg ggcaggtcat cacgcgcccg gtctggccgg
     4921 agccgactgc ccgggcattg accgtgcggt ccgtccacaa cggtaccgat atcgcgtttt
     4981 tgttggagtg gcaggacaat acgaaaaacg accggctcac gcctggcacg ttccgcgacg
     5041 gcgtcgcgat cggcttgcca cttggcgatg caccggcatt tttttgcatg ggccaactcg
     5101 accactacat caatatttgg cactggaagg ccgattggca gagcgatatc gaccgccgtg
     5161 cgtcccgaag caatgacaaa cagcgggaag gtgtgcggac gttcgaggtg atcccccgtc
     5221 gagtgtcttc tgtcgaagat ttgatcggcg gcgggtttag cacactcacg acaaaagaaa
     5281 agcaaggccg tgtccagggc aaggcgacct ggaaggacgg agtctggcac gtagtcatgc
     5341 gccggccgct gggcgccgaa gaacaagaca acgaggcgaa gctcgttccc ggccgcgtcc
     5401 aaaccgtctc cttcgcggtc tggaacgggg aaaacaagga acgaaacggc caaaaagcgg
     5461 tggcgccgtg gtttcaattg gtcattgatc cgattgcgaa aatttaagtt tcatggtgtg
     5521 atgcacgaga gaccatcgcg cgggtaatcg ggcatcacga ggcgccgact ttggaggaac
     5581 atcgctacag gggtatgagt ggcaccgcaa gggcccttcg ttggggaagg gcccttgttg
     5641 tgtgtggggt attattgggc ctgttgggca ttccgcttcc cggacgaacg gcgaacgatc
     5701 gagcggccga cggcatgacg gtggacgagg cggcgaaact gggtgaagac ttcggcgtca
     5761 tcgtcggcga tgtcgacgag gagattcaga aagagttgaa gctgcagcgg ccccaaggcg
     5821 tggcggtgtt cgaagtcatc ggcaattcac gggcagatta tgccggcatc aaagtccggt
     5881 cggtcatcaa ggaaatcgac aagaaagaag ttaggaacat gctcgacttc ggacggcaca
     5941 tcaagaaagc catcaacgaa tgcaatttca cggtcggcac ctacgagcct gccgaccctg
     6001 gcgaccccgt cggttgggga gtgaattttc actttgtcgg ttgcaagcgg gattaggatg
     6061 tggggaaaac gctcttctca cccggccagt ctagcgtctc ggcgcttcgc ggggtaggtg
     6121 ggtgaagata agactgtttt gaacctctag tcaccggaga ggatttcacg ttcgatgcag
     6181 tttcgacgaa tgctgaccaa aagacgcata gccgcaacct gtctcgtgct gcttctcttc
     6241 ggcgtcatcg ccttctatca agatagagcg gtggcccaaa aggccgacgg tcaggccctg
     6301 aatgggcagg cgtcggatgg ctgggtcggc gagatcgagc aggtcttcat ccgctccgaa
     6361 gagtgcaagc agtgccacga ccgccattac gaagaatgga agggtgcacg ggaacagacg
     6421 ccggatctca aaacctttgg ccgggtggac gccgcgcttc tgcacggcac gtcgctggag
     6481 tcgccggtgt ttcgtaccgt gctgggagtg tggctgcaga ccaatcccac ggcggaagaa
     6541 cgcaagcgtt gcctctcatg ccatgtgcca gcgacgaccg tgttcccgca gcatgccgaa
     6601 aaaatcaccg cgcagatctt gtcgggtaca ccccagatcg agggcatcgg gtgcgcttcc
     6661 tgccatctca ttaaatccgt cgagacgacg atgggtccgc ctccgacgtt caagatcgag
     6721 ccagggaaga ccatgcacgg tccctatgcg gatccggagg aaaacctcgt gcatccggct
     6781 gtgcagtcgc cgctcttccg aggtgccaac tactgcgcct cctgccattt cgacaaggtc
     6841 aaggacgtga cgcagaagga tttaccgggt gagattctgc aagggacgat ctgtcaggat
     6901 tgtcacatgg agcagtctac ggggagttcg acttcgaagc ggggcgccat gacgcgctcg
     6961 atcgggcgcc attatttccg gggcgtggtg atccccggca tcctgctcaa gaatcgcaac
     7021 ctccaggccg agtggatgcc gcgcatcgac atcgacagca caaaatccgc cggcaaggtt
     7081 gagggcaccg cgctggtcaa agtcggcagt ctcccgcata gcttcccgga cggcgatccg
     7141 gtgctcaagc aattcatgct ggccattgcg gtaaaagatg cgcgagggaa ggtgctggct
     7201 caagaaacga aacagttcgg actgccgtat gacaagatcc ttcggggacc gattcccgac
     7261 ccattcatta aagggggcaa tacgaggaag gtgccgttct cgctggctgt gccggggggg
     7321 gcgtcagcgg cctccgtgga agcggtgctg agctattcgc tgattccgac gcctgagccg
     7381 gcattgaaag acaagtatct tgcaacgctc aaggacgaca aggagcggga gaaggccaag
     7441 aagatcctcg acgaatacac acagcctcgt atgctgacct accgggcgaa gaccttctga
     7501 cggatttgga gcagcggaac ccatgagcgt gcgcaagacc attgtgccgg tagccttgac
     7561 cgcgatgttt ttggtcgcgg gctggctgtc tgcgcccctg ttcggcgacc ggattttcgc
     7621 gcagcaggac gggaaagcgc ccatcacgat ggacaaagcc tttcccaact ccaacaagtg
     7681 caagcgctgc catgagcgcg tatttgagga gtgggaaacc tctcccttgt cacggtcgat
     7741 ccattcgccc gcttttcgcg cctcccttga cgcctttctg tcatcgcctg ccgggaagga
     7801 caaggcgctc tgcttccgct gccacgcgcc acacgtgcgg gaatttcccg atcaggtgca
     7861 gctcttcatc gatcaggcga aatcgggcga tccgcagatg gacggtgtgg cctgctccca
     7921 atgtcacctc atcaagcagg tggatcgcac caaacagccg ccggagccga agtatgagcc
     7981 ggccggcagc aaaacgctct atgggcccta taaagatttt gcgcaaaatc ttgcccacca
     8041 gtcgttggaa ttaggtctgt tccgaaaatc agacctctgc ttgaactgcc atcaggcggt
     8101 gccatcggcg gccaacctcg gcaaatcgaa cgatttgctc ggcaactggg accagagcaa
     8161 ggccatcaag tcaggcaagg aatgccagtc ctgccacatg cccgagcaga cgggcgaatc
     8221 agccaacggc gaaaagaagc gcaccgtcgc caatcataca ttcccaggtc gtatcggcaa
     8281 gctgcgccag gaagcggcga agcttgatat cgagacaaag attgagggag agaagacgac
     8341 cgtcgtgcta aaagtgcaga gcctcgtccc gcacaacctg ccgacgaccc atccggcttg
     8401 ggcgagtgtc gtgctgcaac tggatatcaa aggtaagaat ttgaagacgg tgttttcgga
     8461 caagcgcgtg tatggcagag tctttcagga cgccaaagga cagaagacgg tcttcgactt
     8521 cgaagccgtg aaggtgctgg aagatacggt gttgaagccc gaagaactgc gcgtcgaaac
     8581 ctttacgttc cccactccga aggacacgcg aacgttcgac gtggaagcga cgctcagcta
     8641 tgcgccggtc agcgggccgg catccttcct tcagcgcatc gaagccgagt cctccaaggg
     8701 gtcttccgat ccggtgttcc aaccaatcga catcgtgaag aaaaccgaaa acattcccgt
     8761 caacaagtag tctgtctgtc caggctcctc ctccaatttg tcttttcaaa caaagagttg
     8821 gggaccaggc tgcctcaaag cgtgagctag ggaacccggc tctggcggac ggacagaggg
     8881 gggagtaggc tgtccccatg cgtctcagca agacatcggt catcgtggtc ggggctggcc
     8941 tggccggact ttccgctgcc gtccggttga tgaaggatgg cgcgcacgtg acggttttgg
     9001 aggcccggga ccgcgtcggc gggagggtcc tgacggtccg cgacgccttt gccagaggcc
     9061 agcatgcgga ggcgggaggc gattttatcg acgacagcca ggaagacatc aggcggctcg
     9121 cggaccagta cggcctgacc gtgcgcacga ttctgcgcca gggattttcc ttcgtgcgcg
     9181 acaagggaaa agggcaaatc catggccgtc cattgaaggg agagcgggcc tggaagccga
     9241 tcgtcgaccg cgccaagcct ttgatcgaag cctatcggag ggcggagcaa caatgggaga
     9301 gcgccgtcgt caggagcata gcccagcgat cggtcgctca atggctcgat gaaattgacg
     9361 ccgatgggaa catgcgggcg atggtccgcg gcctgcgcgg gttcttcctc gccgatccgg
     9421 aggacctttc gctgctggtc ctgattgaac agcttgcctc cgaagcgcca ggacaggaag
     9481 ggatgggacg catcgaaggc ggcaacgatc gattgccgag ggtcatggct gtggaattgg
     9541 gcgagtcgtt gcacctgaac acggcggccc gcgcgatcca cgacgatcag ccgttcgtgc
     9601 acgtgacggt cgagagcgcc ggcggggatc ggacgcagat caaggcagac tatgtcattc
     9661 tctccgtgcc ggccaccgcg cttcgtgcca ttgagatcac gcccgatctg ccgctcgagc
     9721 aggcgaaagt ctttgagcga ttgaagtacg gccgggtcac caagagtctg ctgcaatttg
     9781 accggcgttt ctggaagcgc aagggccgtt cgcctgccta tggcaccgac gccccaaccg
     9841 gcgccatctg ggacgccaat gaggagcaga gaggctccgc ggggatcctg acgctcatgg
     9901 ctggcggcca ggcgagcgaa gacacccaaa agattttggc ccaagagggc gtcgggggtt
     9961 tagtgcgtgc cttggagtgg ttaaagccgg gggatgccaa gctgctgcac agccatcatg
    10021 tgacgtggga gcacgattcg tggtcccaag gcggctacgc ctatttcgat ccttccttcg
    10081 atccggcctg gcggccgtgg ctggcccgtc ctcacgggcg gctgctgttt gccggtgagc
    10141 acaccagcat caagtggcaa ggctatatga acggggcggt ggagagcggc ctccgcgccg
    10201 cggcggaagt gtgggcgctc gcggcggcgg gcaaccgtcg cgctccctag cgctcgctat
    10261 tcgtagacaa tccgcgaacc cggcgacttc atcttgaaga tctgaagcgc gttgtaaata
    10321 cctttggcgg gatggttgag gatcaagcgt gaattcgtga tgtgggtatc gagcagttcg
    10381 tactgcccgc cgctgtagta gagatcgcaa tcatcgatgt tgcaattctt gtagacgtgg
    10441 ttgtccagcg ccaacttggt cttgctgaac gtctgcccgt cgacgacgat gtaattcggc
    10501 tccaagccca tccctgtttc tcccgcgcgg actataacaa agtcttccc
//
LOCUS       DASSCQ010000163        13045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1521604, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000163 DASSCQ010000000
VERSION     DASSCQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13045
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521604"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFH05_11275"
     CDS             complement(<1..368)
                     /locus_tag="VFH05_11275"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET6678844.1"
                     /translation="MKKATVSSPIDARCHTGVEGLDEVVAEGLPCNCFYLIQGDPGSG
                     KTTLALQFLLEGVRRGEKTLYITLSETKEELLKVAHSHGWSLDSIGLFELSAIEALVR
                     PDAHTTVFHPSEIELTKVTD"
     gene            complement(585..890)
                     /locus_tag="VFH05_11280"
     CDS             complement(585..890)
                     /locus_tag="VFH05_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678845.1"
                     /translation="MYRFGQENVARFDPLSGITDFSEVTEVAFSPQTMRDLARTEPIM
                     RDPRRLVIFVAPTPDLFGMARMFELEGAEARPNLRVVHTAEEAYQILNVRDPQFSPF"
     gene            1141..1707
                     /locus_tag="VFH05_11285"
     CDS             1141..1707
                     /locus_tag="VFH05_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET6678846.1"
                     /translation="MAVESVMLPLGTTAPSFFLPDVVNQQVYSLDSFHGKDALLVMFI
                     CRHCPYVKHVEQEIARIGKDYQHKKLGIVGISSNDPATYPDDAPDRLKEMAKRLDFRF
                     PFCFDESQDVAKSYKATCTPDFFLFDAQRRLVYRGQLDDSRPGNNKPVTGRDLRAAID
                     AVLAGKPVDRTQQPSIGCSIKWKEGRMG"
     gene            2186..2542
                     /locus_tag="VFH05_11290"
     CDS             2186..2542
                     /locus_tag="VFH05_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678847.1"
                     /translation="MKDTSRAFMWRMRTVTMLTAVTLLIAFGGNTASADDSGSASGAG
                     LQAASWIATVPYGAVKVVYALSGGVVGSLAWMMSGGNTEVAKAIWNPSMTGHYIVQPQ
                     NLTGDKALHFIGGSSD"
     gene            complement(2581..3843)
                     /locus_tag="VFH05_11295"
     CDS             complement(2581..3843)
                     /locus_tag="VFH05_11295"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HET6678848.1"
                     /translation="MIRDPAAESSFSHYEHARAMATGLLVCMAGLFVAALIWEPRYPS
                     LSWLRAFAEAAMVGGLADWYAVTVLFRRPLGLPIPHTEIIPRNQARIATRLGSLTQRQ
                     LLTPDAVGKLIASWRIPDECMAVLLDPARRLLLTQEAARLLARMVEASEDAAMQQFTR
                     HLATRMLRGLHIAPLIGQVLTGFLQSGQRDRLVNDVLTLAHDSVEAHREQVCRLIADK
                     LPWSRFLTFVRLDEKVAGKLVDWFITVLQEMRDHPEDPMRQEFIERLEAAAQWLMHSE
                     QALEREASIKEKLLAYDALLRFLDESWHSLKNWLVADLRREESEIRAYLDAALAGLGE
                     TLKSDGEFVTLVEQGLQDVVLDLATRHRDRIGELVTATVRDWSVVHMVDTIEREVGTD
                     LQFIRINGAVVGGLVGLLLHGAALLLSK"
     gene            complement(4065..4235)
                     /locus_tag="VFH05_11300"
     CDS             complement(4065..4235)
                     /locus_tag="VFH05_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678849.1"
                     /translation="MSKLGRPSKALKAFVWGCESLLAAVESGSEFSLEELHLIDLYAE
                     VIQQTSAAGLFH"
     gene            complement(4448..5728)
                     /locus_tag="VFH05_11305"
     CDS             complement(4448..5728)
                     /locus_tag="VFH05_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6678850.1"
                     /translation="MSTENRWLLTRDFRLIWWSQVLSQVADGVSRLALLWFVYSVTGS
                     ALQTSIVGLLQTLSPIVLGPFIGVTVDRLPKKAILIVTDVARGILIGLIPCVLSIEVF
                     TVDMLYVLVFLYGIATAMFVPTLSSSVPFLVDRSRFVAANAMLQSTTSIGIIIGPVLS
                     GLGIAFSGSQDVLCANALIYFASAVCLVPIRLPWSRLPHPQGGTIAATFQDLMEGLRY
                     ALVSQRTIVVLILLASIYTFAAGAYTTLFPVFAKKMLALGPVEVGYLWSWLGIGLFLV
                     SLALVPLSKWDSSRRVLVVAVSSVIGGAALCGLVWTQSLVVATVLVTIIGMGFGTWTP
                     IAWSMIQEFVPAHMVGRVMAIYTAVATATSMAGISFFGWVTERADEYISVIGIGCVLF
                     LLGLGSAWFSRSVEGNGRNATPIAPVLPECARGT"
     gene            complement(5884..7188)
                     /locus_tag="VFH05_11310"
     CDS             complement(5884..7188)
                     /locus_tag="VFH05_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6678851.1"
                     /translation="MMRRKPEHDVVPPESGDPESRGWRLIQTRDFGLLWWGQVTSQIG
                     EGLNKVALLWFVYELTGSALMIATVGLIQTVPPLIFGPLIGVYLDRLRKKPIMVWVDL
                     IRTVLTVLIPALYGLGLLRLEGLFILIFLTSMVSTVFGPALVSAVPLLVRRGELVSAN
                     ALIQSTNNIGTLLGPAVSGILIALIGAQHVLYVNAATFLVSALCLIPIRCHETVPERQ
                     QQNADSATSVFRDLLVGFRFVFGDRSTVLLLVTITALYNLGASAFVFVLPVYAKELLH
                     MGPIQLGWLWSALGVGMLTASSWLALRQSTDAQSRMKILVSGTTMGGLATCTLGLLES
                     PLLAAGLVILVGAGAALLNPVVFALLQELTPSHLMGRVLTTFSTGSMAAAMVGMSGFG
                     WIADKIGPAESLVGLGLVLLLTASVAMLFSRKAMDTSMAATA"
     gene            7832..8794
                     /locus_tag="VFH05_11315"
     CDS             7832..8794
                     /locus_tag="VFH05_11315"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6678852.1"
                     /translation="MVQQARRTRPTRAQRSERPWQMVKSLAKKSVDKWNDEPGPRFAA
                     ALAFYTAFTIVPFVVLVVMVSTTFMGEEAIQGDLHKHVGDLIGEPSANGLFHLIDQWR
                     ESGSPVLNEMVALGVFLLGTVHVMDQLKDSLDCIWGLKTKRPRTMMARLRQRFASMSG
                     LLGIAFILLSSLTLSAWMSIGIQSGVGLVGGPMWIQKVIETIISFVIVAALFALIYKW
                     VPQAKVAWADVWIGSGITAMLYTAGSVLIVVKLGHSPLVTFYGGAGSLVLVLLWAYYA
                     SQIFLFGASFIAVYASQYGSHVRPAEGAVAVHSAAKGESADEED"
     gene            complement(8799..9170)
                     /locus_tag="VFH05_11320"
     CDS             complement(8799..9170)
                     /locus_tag="VFH05_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678853.1"
                     /translation="MRSPGVLWIKAAVVYLVMGVGLGIYMGASGDHVMVPVHAHFNLL
                     GWASLALVGLIYHQFPAAGSSRLATVQFWLHNIGLLAAMALLVSALRGNTGLDPFLGL
                     ASVIIGSAIVLFAINVFQRVR"
     gene            complement(9263..9826)
                     /locus_tag="VFH05_11325"
     CDS             complement(9263..9826)
                     /locus_tag="VFH05_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678854.1"
                     /translation="MTTHQFAVDREERVDARSALLAAPGRSPEIPESADAYGWLVGSW
                     NLDVRVYWAMDVSAQRLKGEAHFGWALEGRAIQDVWIMPCVADRTANLDKRLNMYGTT
                     LRVWDPSIQAWRITWINPAGDHREEQIGRWSGKNVVQVGVRPDGTPTRWMFSDITTNS
                     FHWTGEALNPDGHTWRLEGDFRVTRMR"
     gene            complement(10026..10442)
                     /locus_tag="VFH05_11330"
     CDS             complement(10026..10442)
                     /locus_tag="VFH05_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678855.1"
                     /translation="MGHFYRHVLTMFFSLAVIASAFAGDRLSVRTILERPADYQAKVV
                     TVEGTAKSITSAQVHRGAHRCAGSPVYDTQSFMLEDESGTIRVGTAGTCQPNATQPVR
                     ENEHLRIRGVVVTDEKDPKGIPVLYADAIDRITTSP"
     gene            complement(10613..11005)
                     /locus_tag="VFH05_11335"
     CDS             complement(10613..11005)
                     /locus_tag="VFH05_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HET6678856.1"
                     /translation="MKVTPVITGVFLGILCTVVGLAAEPAEVEKYVKARIEIGEMMTN
                     YFKGGEAAPPTQEQRREMGKDINAKLSTVLSKYGLTVEEYRAHSRDVFADEAAVTGFL
                     NEHPDLKQRYDALPLDRMGRGGSSGRGY"
     gene            complement(11521..12762)
                     /locus_tag="VFH05_11340"
     CDS             complement(11521..12762)
                     /locus_tag="VFH05_11340"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET6678857.1"
                     /translation="MTRVSGKPIEIRVSQPTAGSDEPTFPPYNGPAFFSYGFRPFFLG
                     AAVFAGAAVPVWILILAGAMGPNFLYPPREWHVHEMLFGFLPAVMTGFLLTAIPNWTG
                     RLPLRGMPLVLLWVLWLAGRLAMAAPGSVPFLSAVIDASFLVMLAALVWHEIAAAGMW
                     NRSPIGALITLYAAANMLFHTFALHAVPTDAPERLALSILFMLLTVIGGRVTPAFTGE
                     YLKEQRITPPPVPFSRFDGLSMLLVLIAILAWNVQPESYAAGALFLAAGVATLVRLSG
                     WRGWLAWREPLVFILTVGYGWVGLSLLALGGASLGMLPAANAVHVLTTGAVGTMTLAV
                     MTRASLGHTGRPRHAGLMTVVIYVLVNIGAVLRIVSPASDAPTALTLLLLGLAAVAWS
                     GAYVLFAMVYGPVLVRASVEE"
BASE COUNT         3006 a         3887 c         3619 g         2533 t
ORIGIN      
        1 tgatcggtca ccttggtcaa ctcgatttcg gagggatgga acaccgtcgt gtgcgcgtca
       61 ggacgaacga gtgcttcgat cgcggataat tcaaacaaac cgatgctgtc cagcgaccac
      121 ccatgcgagt gcgcaacttt aagcaactcc tctttcgttt ccgagagcgt gatgtaaagg
      181 gtcttttctc cgcggcgtac tccctccaga aggaactgca gggccaaggt ggtctttccc
      241 gagcctgggt cgccctgaat gagatagaag caattacagg gcaatccctc agccactact
      301 tcatcaagtc cttccacgcc ggtgtggcaa cgtgcgtcga tgggagagga gacggtcgct
      361 tttttcatac tcgtccaacc agcctttctg gctttacctg agtagatcaa aagtatgggt
      421 gtgttcttaa gaagccgatg ggacctattc ataccgcgac tctgtaggag ccttgtccta
      481 ggggaacccc tagcttttag cgtcctgggt cattgaagcg gaagtcagtg tggtaggcag
      541 tcttgtaagg gggccccggt accaagaaac agccatgaag aggttcagaa aggcgagaat
      601 tgagggtccc ggacgttcaa gatctgatag gcttcttccg ccgtgtgcac gacacgcaga
      661 ttcggacgag cctctgcacc ctccaactcg aacattcgcg ccatcccaaa aagatcaggg
      721 gtcggcgcca cgaagatcac aagccggcgg ggatcgcgca taattggctc ggtccgggcg
      781 aggtcgcgca tcgtctgcgg agagaacgcc acctccgtca cctcggaaaa atcggtgatg
      841 cccgagaggg ggtcgaaccg ggcgacgttc tcttggccga atcgatacat gtccctcagg
      901 tcgtcatcag tcacccgccc tatacaccgc gctcgaagga tccgattggt tgcgtcgaag
      961 tcgatcagat agggcacttg gctcctttga cgtgagtttc tagttgccag cgagaacgac
     1021 tggcgagaca atagccgacg tgggaggtac agtaacatgt tcgggtatga accgatgtcc
     1081 gtataacgga ctcagatact gtcctacata agcgaacgaa cgcgatccaa cgaggactcc
     1141 atggctgttg aatccgtcat gcttccgctc ggaaccactg ctccgtcgtt tttcttgcca
     1201 gacgtggtca accagcaggt gtattcgctc gactcgtttc atggcaagga tgccctgctc
     1261 gtcatgttca tctgccggca ctgtccctac gtgaagcacg tcgaacagga gatcgcccgg
     1321 attggcaagg actatcagca taaaaagctt ggcattgtgg gcatcagcag caacgacccc
     1381 gcgacgtatc ccgatgacgc cccggaccgg ctgaaagaga tggctaaacg gctcgatttc
     1441 cgtttcccct tctgtttcga cgagtcgcag gatgttgcca agagttacaa ggccacctgt
     1501 acgcctgact tctttctctt tgatgcgcag cggcgattgg tgtatcgcgg gcagctcgac
     1561 gacagccggc cgggcaacaa caagccggtg accggtcgcg atctgcgtgc cgcgatcgac
     1621 gccgtgcttg ccggcaagcc ggtggaccgc acgcaacagc ctagcatcgg ctgctccatc
     1681 aagtggaaag aggggcgtat gggctgatga tctcctgtgc tcgccgaacg cgcacaccag
     1741 atatgggtgg ctcagcctgg tcagtccttg tgctcgcgga aggcggaaca ccagaaacac
     1801 aaagatggat cgccagccac gctggtcctt tgctccccga acgcgcacgc aatgccgatc
     1861 atatttctag atctagggcg gtgctcgtcc aagggcgctg taggaccagc atggctacca
     1921 ctccatcaag aggagacata agcaagcttt tgaaggaaca tccaaacgcc gcacgtttgg
     1981 actgatccag gctgctcctt ggcccgaaca ggccagcttt cagggaacaa cgcgcagtgg
     2041 agatcattca gcccgcccag gagatcgaac gagcgagctt ggaaggagca tttagagcct
     2101 tcatctccct gaggcttgcc tcatcatcct tttgatatat aatgcggccg ttcatcgcga
     2161 cccacctcaa acgaggagaa caagcatgaa agacaccagc cgtgcattca tgtggcgcat
     2221 gcgcactgtc acgatgctca ccgccgtcac gctgctcatc gcattcgggg ggaacaccgc
     2281 ttcagcagat gattcgggaa gcgccagcgg agcgggattg caagcggcga gctggatcgc
     2341 gaccgtaccc tacggtgcag tgaaggtcgt ctatgccctc agtggaggcg tggtaggcag
     2401 cctggcctgg atgatgtcag gaggtaatac cgaggtcgcc aaggcgatct ggaatccttc
     2461 gatgacgggc cactacattg tgcagccgca aaacctgacg ggcgacaagg ccctccattt
     2521 catcggcggc tcctccgact aaacggtcgg tccgcgccag tgcctcaggg gctgctcagc
     2581 tcacttgctc aggagcaggg cggcaccgtg aagcagcagt cccacgagac cgccgacgac
     2641 cgcgccgttg atgcggatga actgcaggtc ggtgcccacc tctcgctcga tcgtgtccac
     2701 catgtggacg accgaccagt cgcggaccgt tgccgtgacc aattcgccga tcctgtctcg
     2761 atgccttgtg gccagatcca gcacgacatc ctgtaatcct tgttcgacca gcgtgacgaa
     2821 ttcaccatcc gacttgagtg tctcaccgag accggctaag gctgcatcga gataggcgcg
     2881 gatctccgac tcctcccgtc ttagatccgc caccaaccaa tttttcaagc tgtgccagga
     2941 ttcgtcgagg aatcgcagca gcgcgtcgta ggccaagagc ttctctttga tggacgcctc
     3001 ccgctccaag gcttgctccg agtgcatcaa ccactgcgcc gccgcctcca ggcgctcgat
     3061 gaactcttgt ctcatcggat cctcgggatg gtctcgcatc tcctgcaaga ccgtgatgaa
     3121 ccagtccacc agcttgccgg cgactttttc atcgagcctg acaaacgtga gaaatcggga
     3181 ccacggcagc ttgtctgcaa tgagtcggca cacctgttcc cgatgagctt cgacactgtc
     3241 gtgtgccaac gtgaggacgt cattcacgag ccggtcccgc tgcccactct gtaaaaaacc
     3301 ggtcagcacc tgccctatca gcggcgcgat gtgtaggcca cgcaacatcc tggtcgcgag
     3361 gtgacgggtg aactgctgca tcgcggcatc ttcagacgcc tcaaccatgc gggccaacag
     3421 gcgcgccgct tcctgagtca ggagcagcct tcgcgccgga tcaaggagca ccgccataca
     3481 ctcgtcgggt atgcgccagg acgcaatcag ttttcccacg gcatccggtg tgaggagttg
     3541 gcgttgcgtc aggctgccaa gcctggtcgc gatgcgcgcc tggtttcgcg ggatgatctc
     3601 cgtatgcgga atcggcagac cgagcggccg tctgaacagg acggtgacgg cataccagtc
     3661 ggcaagtcca ccaaccatcg ccgcttcggc aaacgcccga agccagctca gcgacggata
     3721 cctgggctcc cagatcagcg cagccacgaa cagacccgcc atacacacca gcagtcctgt
     3781 cgccatcgcg cgggcatgct cgtagtgact aaaagacgac tcggctgcgg gatcacgaat
     3841 catgatgcac ctctgttatt tttgtgaaaa ctcgtttcgt gatctgacct gttcgaccac
     3901 ggaccgatcc agaccatcca tgagcaagaa agccgagcat gctgtgcggg atcatcgtat
     3961 tctaacgcga gcgggtgacc ggctgggaag gtgttcctta cagagaagaa atgcggttct
     4021 acggtgcccc gactctcccg atgaaagtca ggtgcaacgt gacgtcagtg aaagagacct
     4081 gcagctgagg tctgctggat cacttctgca tagaggtcga tcaggtgcag ttcttctaac
     4141 gagaactcgc tgcccgattc gaccgcggcc agaagagatt cacaacccca aacaaaggct
     4201 ttcagcgcct tagatggtct tccaagcttg ctcatatact ttcttaaagg gagccccgat
     4261 tggtctccaa ctgcgcgaac attctcaccc gcccaaccct gcgcacgccg agacgtgcgc
     4321 ttccccacgc gagcaccgcc ttataaatgg tcgctcccag ctcgctcgta cgctttctaa
     4381 ggaatgacac gcctgtcgct catactgcgc ccgtggaggg agcacctccc ttaatttcaa
     4441 aatccgatca agtcccgcgt gcgcactccg ggagcacagg agcgataggc gtggcattcc
     4501 tcccattccc ctccacactc ctgctgaacc atgccgaccc aaggcccaac aggaaaagta
     4561 cgcacccgat tccaataaca ctaatgtatt cgtcggcccg ctccgtgacc cacccgaaaa
     4621 aggagatgcc ggccattgat gttgccgtcg cgactgccgt atagatcgcc atcactcgcc
     4681 ccaccatgtg agccggtacg aattcctgga tcatgctcca ggcgatcgga gtccatgtgc
     4741 caaatcccat accgatgatg gtgacgagca ctgtcgcgac caccaggctc tgtgtccaga
     4801 ccagcccgca gagcgcggct cctccgatca cgctcgacac cgcgacgacg agcacccgtc
     4861 tgctgctgtc ccatttgctc aagggcacga gcgccagtga gaccagaaac aagccgatgc
     4921 cgagccagga ccagagataa ccaacttcca ccggacccag ggccagcatc ttcttggcaa
     4981 agaccgggaa taacgtcgta tacgcaccgg ccgcaaacgt atagattgac gccaagagaa
     5041 tcagcacgac gatggtccgc tgagagacca gcgcatagcg caatccttcc atcaaatctt
     5101 gaaaggtcgc ggcgatggtt ccgccctgcg gatggggcaa ccggctccaa ggaagccgga
     5161 tcggcacgag acaaactgcc gacgcaaaat agatcagcgc attggcgcaa agaacgtctt
     5221 gtgaaccaga gaacgcaatc ccgagaccgc tgagtacggg tccgataatg atgccgatgc
     5281 tcgtcgtact ctgcagcatc gcgttagccg cgacgaatcg agatcgatcg accagaaagg
     5341 ggacggacga ggacagtgtc ggcacaaaca tcgccgtcgc gatgccatag aggaacacca
     5401 ggacatacag catgtcgacc gtaaagactt cgatcgagag cacacagggg atcagcccga
     5461 tcagaatccc ccgggcgacg tcggtgacga tcaagatcgc ttttttgggc aggcggtcaa
     5521 ccgtgacgcc aatgaacggt cctaagacaa tgggcgagag cgtctggagc aggccaacga
     5581 tgctggtttg cagtgccgat cccgtcactg aatagacgaa ccagagaagc gccagccggc
     5641 tcacgccatc ggccacttgc gacagcacct gactccacca aattaggcgg aagtcacgtg
     5701 tgaggagcca gcgattctca gtcgacatgt caacaccttg tgttctgaag atagcggttt
     5761 tctgcgctga aagacagagt gcgaagaggc ggaacgagca agcttggagg gaatattgta
     5821 aaggagctcg ccgcggctga accagacggc gagcttcggt agagacgtaa aagagcgtga
     5881 tagttaagca gtggccgcca tcgacgtgtc catcgccttg cgtgagaaaa gcatggctac
     5941 ggacgccgtc aggagcaaca caaggccgag cccgacgaga ctctccgctg gtccgatttt
     6001 atctgcgatc cagccgaatc ccgacatgcc caccattgcg gccgccatcg aacccgtgct
     6061 gaacgtggta agcactcgcc ccatcaggtg cgagggcgtg agttcctgca gcagtgcgaa
     6121 caccaccgga ttaagcagcg ctgctcccgc tcccaccagg atgacgaggc ccgccgcgag
     6181 cagcggggac tcgagcaagc cgagcgtgca cgtggccaac ccccccatcg tggttccact
     6241 gacgaggatt ttcatccgac tctgtgcatc ggtactctgt ctcaaggcca gccatgacga
     6301 cgctgtgagc atgcccactc ccagggcaga ccagagccag cccaattgaa tcggccccat
     6361 atgcagcagc tccttcgcat acaccggcag cacgaagaca aacgcactcg ctccaagatt
     6421 gtatagagcc gtgatggtga ccagcagcaa cacagtcgag cgatcgccga agacgaaccg
     6481 gaatcccacc aggagatccc tgaacaccga cgtcgccgag tctgcgttct gctgctgcct
     6541 ctctggcact gtctcgtgac aacgaatcgg aatcaggcat agcgcagaga cgaggaaggt
     6601 tgccgcattc acgtagagga cgtgttgtgc gccgatcagc gcaatcaaga tcccgctgac
     6661 ggccggcccc aacaaggtcc cgatattgtt cgtgctctga ataagggcat tggcggaaac
     6721 gagctcgcct cgccgaacca acaagggaac agctgagacc aaggcgggac caaacaccgt
     6781 agagaccatc gaggtcagga agatcagaat aaagaggccc tcgagcctca ggagtccgag
     6841 tccatacaag gccggaatga ggacggtcag caccgtacga atcaggtcca cccacaccat
     6901 gatgggtttc tttcgaagac gatcaagata gacgccgatg agcggaccaa aaatcaacgg
     6961 cggcacagtt tgtatcaaac cgaccgtcgc aatcatcagt gcggagccgg tcagttcata
     7021 gacgaaccac agtaaggcaa ccttattgag tccctcacca atctgcgagg tgacctgtcc
     7081 ccaccacaac aggccgaaat cccgggtttg gataagacgc caacccctgc tctcgggatc
     7141 gcccgactcg ggtggcacta cgtcatgttc tggtttccgc ctcatcatgt tcacgcccac
     7201 tccttcttcg ttgttcatgc tgttgagtag agcaaaggac gggccagacg gtatcgtccc
     7261 taaaagaagg tggagatgag caatatcaaa agaattggca aattcctgtg agaggaatac
     7321 gtgtgttcga tcacttgatg gggtgcaaat gcgccacagt ctaagacacg ctgtctgcaa
     7381 caaatccggc gtcactcact cgcaggtttt agggaaagcg tgaagccgcg acaaaaacgt
     7441 cacgctgatg atttgctgac atgtgggcag aagctgacaa atctgtaggc agtgttcgtc
     7501 aattcatagg tgtatttttg cgacaaaaaa tgtaggcgag tcccgctagg agggacgcta
     7561 gaaaacgccg acctaccctc tagccaatgc gtcgtcacaa acgcgtttca agaacgttca
     7621 agaacgctgc catattaact taaagtgaca catgataagt tgtcgcttaa ctcactgaca
     7681 gtctgtcgct tacactcctc tgcgcaggcg cgcttggggc tatatgagat agaggagttg
     7741 gctttctcat tcaacagggt taccgtacat ggaatgctcg cgtgcatcga ggcgcgggct
     7801 gaaactctct ggtcttcctc agaggattcg aatggtacag caagcacgcc gaacccgccc
     7861 tacccgggca cagcgatccg agcggccatg gcagatggtc aagtcgctcg ccaaaaaatc
     7921 agtcgacaaa tggaacgacg agcccggtcc gcgttttgct gcagcgctcg ccttctacac
     7981 agcgttcacc atcgtgccct tcgtcgtgct ggtcgtcatg gtctcaacaa cattcatggg
     8041 agaagaggcg atacagggag atttgcacaa gcacgtcggc gacctcatcg gcgaaccgtc
     8101 ggcgaacgga ctgtttcatc tgatcgacca atggcgggag tctggctccc ctgtgctgaa
     8161 tgaaatggtg gcgttgggcg tcttccttct cggaacggtc catgtcatgg atcaactcaa
     8221 ggactctttg gattgcatct ggggcctgaa gaccaaacga ccacgcacca tgatggcgcg
     8281 gctcaggcag cgattcgcct cgatgtccgg cctcttgggc attgccttca tcctgctctc
     8341 ctcactgacg ctcagcgcct ggatgagcat cggcattcaa tcgggagtgg gactggtagg
     8401 cggaccgatg tggatacaga aagtcatcga gacgatcatt tcgttcgtca tcgtggccgc
     8461 gctcttcgcc ctcatttata aatgggtgcc gcaggcaaaa gtggcctggg ccgacgtctg
     8521 gatcggatcc ggaatcacgg cgatgcttta tacggcggga agcgtgttga tcgtcgtgaa
     8581 actggggcac agcccgttgg tgacgttcta tggaggagcc ggctcgctgg tgctggtgtt
     8641 gttgtgggcc tactacgcct cgcaaatttt tctgttcggc gcctcattca tcgccgtcta
     8701 tgccagccag tatggctcgc atgtccgccc tgcggagggt gctgtcgccg tccattctgc
     8761 tgctaaaggc gagagtgcgg acgaagagga ctgagcgatt atcgaaccct ctgaaacacg
     8821 ttgatggcga acaagacaat ggccgaacca atgattacgg aagcgagccc gagaaacgga
     8881 tccagcccgg tgttgccgcg cagagcactg accaacaagg ccattgctgc gagtaaaccg
     8941 atgttgtgga gccaaaactg aacggtcgcc aaacggctag accccgcggc gggaaattga
     9001 tggtagatca atccgaccag cgccaatgat gcccacccta ggagattgaa atgggcatga
     9061 acaggaacca tgacatgatc accggacgca cccatgtaga tgcccaaccc tactcccatg
     9121 acgagataga ccaccgctgc cttgatccac aagacgccgg gacttcgcat cagagactcc
     9181 tcggttgatg gtacgtagcg cgtagaggtg tgcgaacccg ggcaggatac cccagcggca
     9241 gaattttgca actgcagcga ctctagcgca tccgcgttac acggaaatcg ccttcgagcc
     9301 tccacgtatg accatccgga ttgagggctt caccggtcca atgaaatgaa ttggtcgtga
     9361 tgtcggagaa catccagcga gtgggcgtcc catcgggacg aacccctacc tgcaccacat
     9421 ttttaccact ccagcgaccg atctgctcct cccggtggtc tcccgctgga ttgatccagg
     9481 taatgcgcca agcctgaatc gacgggtccc agactcgcaa ggtcgtgcca tacatgttca
     9541 gccgcttgtc cagattcgcg gtgcggtccg ccacacaggg catgatccac acatcctgaa
     9601 tcgcacgacc ttcgagtgcc cacccgaagt gggcctcgcc tttgagacgt tgcgcactca
     9661 catccatcgc ccaatacaca cggacgtcca agttccagct ccccaccagc catccatagg
     9721 catccgcgga ttcaggaatt tcaggcgagc ggccaggagc cgccaacaat gcgctgcgcg
     9781 cgtccactct ctcttcacga tcaacggcaa attggtgtgt ggtcatggtg cacctccgga
     9841 aaaacgcgtg cgtggtgtga atgataccga ctctggcgta tctgaagaat gtttggagct
     9901 gggaaagcga ctagttgtgt ggccggttaa ccgaacccat tgaacacgaa gaagcctctc
     9961 gtcattgaca gaacccgccg gtcaaagccg aaggcagaag caggaggaca tcgccgtccg
    10021 ggaccttacg gtgatgtcgt gatgcggtcg atggcatccg catagaggac gggaatgccc
    10081 ttggggtcct tttcatccgt caccacgacc ccacgaatgc ggagatgttc gttctcgcga
    10141 accggttgcg tggcattcgg ttggcaggtg ccggccgtcc ccacccgaat ggtgcccgac
    10201 tcatcctcaa gcataaaaga ttgtgtgtca tagacagggc tgccggcaca gcggtgcgcg
    10261 ccccgatgaa cctgagcgct cgtgatagat ttggccgtcc cttcgacagt cactactttg
    10321 gcctgatagt cggcaggacg ctctagaatt gttcggacgg acaggcgatc gccggcgaag
    10381 gccgaagcga tcactgccag agaaaaaaac atcgtcaaga cgtggcgata gaaatgcccc
    10441 attgcgtgat cctccctaac aacatcggtt ctcctaatgg tgatacacca agaatgccgc
    10501 caggccaatc aggaaaccaa tattgagcag agcattcgtc gtggacgacc accctttgcg
    10561 aagtcgatca cgctcatcga gcgaatgtcg atctgtaaca aagtcacggt ctttagtatc
    10621 cgcgtccgct gctgccgccc ctgcccatcc gatccaacgg aagggcgtcg tagcgctgct
    10681 tcagatcggg atgttcgttc aggaagcctg tcaccgctgc ttcgtcggca aagacatctc
    10741 tgctgtgtgc gcgatactcc tccaccgtca gcccgtattt ggacagcacg gtgctcagtt
    10801 tcgcgttaat gtccttgccc atctcgcgcc tctgctcttg agtaggaggg gctgcctctc
    10861 cacctttgaa atagttcgtc atcatctcac cgatctcgat acgggccttc acatacttct
    10921 ccacctctgc cggctcagcc gcaagaccga caacggtaca caggatgccc aggaacactc
    10981 cggtaataac aggggtcact ttcatcgtga aatctcctat ttgttccatt ctcagtgccg
    11041 ctaagtggct tgtcaatgag cgcctgtggc tgattgtcgt aacttcgatg ggtgcagggc
    11101 tcaatatgcg ccttcttccc gttgcaccgc ctataaatgg tccttccaag ctcgcttgct
    11161 ttctcttcag gcggggctgg tttgatctcc actgcgcgct tcgacgagcc atgtaatagt
    11221 ctttcaaagc ttccttgttc cttcccttat cataaggagc agcctggatc agtccaaacg
    11281 tgcgcgcgtg tacgagcacc gactatatat aaatggtcct tccaagctag cttgttgcct
    11341 tccaggaacg acacgcatat cgttcctact gcgcccgtgg agggagcacc tcccgcaatt
    11401 tagatctgaa ttagtcccgc gtgcgcgctc cgggagcaca ggaacgatat gcgtgtcgtt
    11461 ctctcctttc ttgtgcgcgc gttcggcgag cacaggagat taaccagccc tcacgcccct
    11521 ttattcctcg acgctggcac gaacgaggac cggtccatag accatggcga aaagcacata
    11581 ggcaccactc catgcgaccg ccgccaggcc gagcagcaga agcgtcagag cagtcggagc
    11641 gtcggaggcc ggggaaacaa ttcgtaagac cgcaccgatg ttgaccagca catagatgac
    11701 aacagtcatg agaccggcgt gcctggggcg gcctgtgtga cccaaactcg ctcgtgtcat
    11761 aacggccaac gtcatcgttc caacggcgcc ggtcgtgagg acatgcacgg cgttcgcggc
    11821 gggcagcatg ccaaggcttg cacctccgag cgccagcagc gacagcccca cccacccata
    11881 tccaaccgtg agaatgaaca ccaatggctc tcgccacgcc agccatcctc gccaaccgga
    11941 aaggcggaca agggtcgcca cacccgccgc gagaaacaac gcgcctgctg cgtaactttc
    12001 cggctgaacg ttccacgcga gaatggcaat caggacgagc agcatcgaaa ggccatcgaa
    12061 acgggaaaaa ggaacaggcg gaggggtgat gcgttgctcc ttcaggtatt cgccagtgaa
    12121 ggccggtgtc acccgtccac cgatgacggt caacaacatg aaaaggatcg agagcgccag
    12181 gcgttcgggc gcatcggtcg gcacggcatg cagcgcgaat gtgtgaaaca gcatgtttgc
    12241 ggcggcatag agtgtaatca aggccccgat tggcgaccgg ttccacatgc ctgctgccgc
    12301 aatctcatgc cacacgagcg cagccaacat caccagaaag ctcgcatcga tgaccgcgga
    12361 cagaaacgga actgatcccg gtgctgccat ggccaaccgt ccagccagcc acaggaccca
    12421 caacaaaacc aagggcatgc ctctcaacgg aagtcgtccc gtccagtttg gaatcgcggt
    12481 cagaagaaag ccggtcatca cggctggcag gaatccaaac agcatttcgt ggacgtgcca
    12541 ctcgcgcggg ggatagagga agttggggcc catcgcgccg gccaggatca ggatccagac
    12601 aggcacggcg gctccggcga acaccgcggc ccccaaaaag aatggcctga agccatagga
    12661 gaaaaacgcg gggccattgt aaggagggaa ggtcggttca tccgacccgg ctgtcggctg
    12721 gctaaccctg atctcgatcg gcttgcctga cacgcgagtc attctcgaca accggccaaa
    12781 ggattgtcaa ctccgcggaa ggaaatcact gcatcccttt tcaaagaagc gacgcgccaa
    12841 tgaggatcag ggcgtcgcaa gcaggctccc tctccacttt cacgaaatta acgaccgcta
    12901 cccacggatc acaaaacacg aagaaagcaa gcggtgagcg gcttggtcgt cggtgagcgt
    12961 ctcgagaacg tcgcctgtgt ttctttactc ggtgtgcccc gcttcgaagg ccttgaccac
    13021 gagcgttttc tctcgcatga gctcc
//
LOCUS       DASSCQ010000164         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1273852, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000164 DASSCQ010000000
VERSION     DASSCQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273852"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1398
                     /locus_tag="VFH05_11345"
     CDS             34..1398
                     /locus_tag="VFH05_11345"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET6678858.1"
                     /translation="MPEVPVKLYLDKILKVCRNAVRRTSVISGPVKDRALRAMADRVI
                     QDEAAILSANERDVDALGKSFGSEVSKDRLKAAVARVRMTADDVKEMADRLRAIADQP
                     DPVGAVTGRWERPDGLQVSRVRGPIGVIGIISELSPLVTVESIALCLKSGNVSVFRGA
                     PDWSQTHEVIGKGLREAAAQQGIPPDAWVFIDRPEKDVAVELIRSGRALDAIIPRGGA
                     GLRKAVLEGARMPVLCHDGGQTYLYVDADVDLPLVQNVVINSKVQRPAATNALDTLLV
                     QQGVARQFLPALINRLLVEFKIDVRGCPKTLALMGQMPISGHTSIIPAGEEDWSRQFQ
                     SPTMAVKMVTDMDEALTHIAEHGLCMTAVIATSNYSSALRFTREVDASAVFVNASSRL
                     HDAEGFGFGSDIGLSSARLHAKGPIGLEQLTCEKYVVFGNGQLRLPHPVPETYFDAIM
                     LKRP"
     gene            1653..2837
                     /locus_tag="VFH05_11350"
     CDS             1653..2837
                     /locus_tag="VFH05_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6678859.1"
                     /translation="MAHSPLQEALHDHLAQWGLRLFSSDQAYARWQRQELSPDELSAL
                     HQHVERKRHGSSADETAFYDLTASPRILPVLYSQRYEYYVAIGKIVEAHLGEAMTILD
                     FGCGPGILTTFYARRLPHAEVTGIDRSAACIAQAQRKADELGLKNVRFVCADLVEGPA
                     IGTYERVVATHALVQSERDPGLPSISWRTFERARDAQAQAAFEDRTGLGRRLDRVAAA
                     LVPTGRLVVFEKTRQLSRRIPLQRAFSVRGLKLLEPPRPIRYSLVEEVSDDGPLFVLA
                     LGEEPACAWTEDPEPDEGLPFDRVGLTSRSSGPEAPLYENHWPSAQREWEQLHDRKVI
                     KEASAEESDGRQLHVELGTAEGYRYLYCANTFDQRQLVIVEPAQAAMLHTYYQEIIQG
                     SA"
     gene            2847..4067
                     /locus_tag="VFH05_11355"
     CDS             2847..4067
                     /locus_tag="VFH05_11355"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HET6678860.1"
                     /translation="MPAHTMPTSIELLIIIFSALVILSILTIKLSVRLGIPSLTLFLV
                     IGMLAGSDGVGGLYFDNPALVQTLGVIALVLILFSGGLDTEWTGVRPVLWNGLSLSTI
                     GVVLTALLVGSFVSWVQGFSFLEGLLLGAIVASTDAAAVFMVLRARNVKLPRPLAHLL
                     ELESGSNDPMAVVLTVALIQLMTNPSTNLWDLLWFFVVQMTVGAAAGVAMGELIRWTL
                     NTLKLELRGIYPVLSVAFALLTYGLTAELHGSGFLAVYLAGLFMRRHSVVHQKNLVQF
                     HEGLAWLMQITMFLILGLQVFPARLIPIAGVGLLVSLFLIFIARPVSVYTSLALSRLS
                     FREQTLVAWVGLRGAVPIVLATFPVLAGIQKADTIFHLVFFIALTSVLLQGPPIPWMT
                     RLLGLDRVLQEGRR"
     gene            complement(4075..>4765)
                     /locus_tag="VFH05_11360"
     CDS             complement(4075..>4765)
                     /locus_tag="VFH05_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET6678861.1"
                     /translation="VIFRWGRLARGLVGGNGPGVVIIIPFIDKLVRVSLRTVTMDVPP
                     QDVITKDNVTVKVNAVIYFRVVDQERAIIQVEDYLYATSMMSQTTLRSVLGQSQLDDL
                     LSKREQINADLQRIIDQQTEPWGVKVTAVEVKNVDLPQEMQRAIARQAEAERERRAKV
                     IHAEGEFEASQRLADAANVISQNPAALQLRYLQTLVEIAAEKNSTTIFPIPIDTIAPF
                     FKHWTKPADAT"
BASE COUNT          883 a         1345 c         1469 g         1068 t
ORIGIN      
        1 gagggtaatt catcgcacat cacgagggcc tgaatgcctg aagttccagt taagctttac
       61 ttagacaaga tattgaaggt gtgtagaaat gccgtccgca ggacttccgt gatttccggt
      121 ccggtcaagg accgggccct gcgagccatg gccgatcgag tgatccagga tgaggcagcc
      181 attctctcgg cgaacgagag agatgtggac gcgctcggca aatcgtttgg cagtgaggtc
      241 agtaaggacc ggctgaaggc ggccgtcgcc cgggtgcgta tgacggcgga tgatgtcaaa
      301 gaaatggccg atcggcttcg agccatcgcc gaccaacccg atccggtggg cgccgtgacc
      361 ggacgatggg agcgtcctga tgggcttcaa gtgtcgcgtg tgcggggtcc catcggggtt
      421 atcggcatca tttccgaatt gtcaccgctg gtcacggtcg agtcgatcgc cttgtgtctc
      481 aaatccggca atgtgagcgt gtttcgcggc gcacccgatt ggagccaaac gcacgaggtc
      541 atcggcaagg gattgcggga agcagccgcg cagcagggca ttcctccaga cgcctgggta
      601 ttcattgatc ggcccgagaa agacgtggcg gtggaactga tcaggtccgg cagggcgctg
      661 gacgccatca ttccgcgggg aggggccggg ttgcgcaagg cggtgcttga aggagcccgg
      721 atgccggtcc tctgccacga cgggggtcag acgtaccttt atgtggatgc cgacgtggat
      781 ctgccgttgg tgcaaaatgt ggtgatcaat tcaaaagtgc agcgaccggc tgccaccaat
      841 gcgctggata ccttgctggt tcagcagggc gtcgcacggc aattcctgcc cgccttgatc
      901 aaccgtctgc tcgtcgagtt caaaatcgac gtgcgcgggt gcccgaaaac gctggcactc
      961 atgggccaaa tgccgatcag cggccacacg tccatcattc cagccgggga ggaagattgg
     1021 tcacgacagt tccagtcacc cacgatggcc gtgaaaatgg ttaccgacat ggacgaagcg
     1081 ctgacacata ttgccgagca cggtctctgc atgacggccg tgattgcgac ctctaactac
     1141 tcgtccgctc tgcgcttcac gcgcgaagtg gatgccagtg ccgtgtttgt gaacgcctcg
     1201 tcgcgtcttc acgatgcgga aggattcgga ttcggcagcg acatcgggct cagcagtgcg
     1261 cggctccatg ccaagggtcc gatcgggtta gagcagttga cctgtgagaa atacgtcgta
     1321 ttcggaaacg gtcagctgcg cttgcctcat ccggtacccg agacgtactt tgatgccatt
     1381 atgctcaaac gtccgtgaga ggatgttgaa aacgcccgcc tgcgtcgctc ctacatcgtt
     1441 cggatcctcg acgcaccact tgggcaaatg cctgttctac aggcggtggg tgggtgagat
     1501 ggcgtacgtc tcggacataa aacactcggc gctcatcact cgcgcccgtc cgcaaacgtc
     1561 acgttcatga gtcttcgcgt cgcggacctc actgcggcct aacgggcagg cgttttgaat
     1621 atccttcgca cgtgatcctg ctcagaggta ccttggccca ctcgcctttg caagaagcgc
     1681 ttcacgatca tcttgcccaa tggggcttgc gactattttc gagcgaccag gcgtacgctc
     1741 gctggcagcg gcaggagttg tctccggacg aactttcagc cttgcatcaa catgtcgaac
     1801 gaaagcggca tggctcttcc gccgacgaaa cggccttcta cgatctgacc gcgtcaccac
     1861 gcattcttcc agtcctttac agccagcgtt atgagtacta tgtggcgatt gggaagatcg
     1921 tagaggctca tctgggcgag gccatgacga ttcttgattt cggatgcggt cccggaatcc
     1981 tcacaacatt ttatgcccgg cggcttccgc atgccgaagt cacaggcatc gatcggtcag
     2041 ccgcctgcat cgctcaggcc caacgcaagg ccgacgaatt gggtctgaag aacgttcggt
     2101 ttgtgtgtgc cgatctcgtc gaaggaccag ccatcgggac atacgaacgg gtcgtcgcca
     2161 cccatgccct cgttcaatcg gagcgggatc ccggactacc aagcatcagc tggaggacat
     2221 tcgaacgggc gcgggatgcg caagcgcaag cggcgtttga ggaccgaaca gggctcggtc
     2281 gccgtcttga tcgggtggcc gccgctctgg ttccgaccgg acgtctcgtc gtgttcgaaa
     2341 aaacgcgtca actgtcgcgg cggattcccc tacagcgggc gttttcggta cgcggcctga
     2401 agctgctcga gccgccgcgc cccatccgct attcgttggt tgaagaggtc tctgacgacg
     2461 ggccgctgtt tgtgctcgcg ctcggggagg aacctgcgtg tgcctggact gaggacccgg
     2521 agccggatga ggggctgccg tttgatcgtg taggcctgac atcccgttct tcaggtccgg
     2581 aggcgccgct ctatgaaaac cattggccat cggcgcagcg ggagtgggag cagctacacg
     2641 ataggaaagt gatcaaggaa gcgtctgccg aagaatccga cggccgtcaa cttcatgtcg
     2701 aactgggcac cgccgaaggc tatcgctacc tgtattgcgc caatacattc gaccagcgcc
     2761 agttggtgat tgtcgaaccg gcgcaagccg cgatgcttca tacctattac caggaaatca
     2821 ttcaaggcag tgcttgacgg agtgtgatgc cagcacacac catgccgaca tcgatagagc
     2881 tgttgatcat catattttcc gcattggtta ttcttagtat tctcacgatc aaattgtccg
     2941 tcaggttggg gattccgtcg ctgactctct ttttggtgat cggcatgctg gccggttccg
     3001 acggggtggg cgggctgtac ttcgacaatc ccgctcttgt ccagacgttg ggagtcatcg
     3061 cactcgtctt gatcctgttt tccggcggac tggataccga atggaccggt gtgcggccgg
     3121 ttttatggaa cggcctctcg ctctccacca tcggcgtcgt gctcacggcg ctgctcgtgg
     3181 gctcgtttgt gtcctgggtg caggggttct cgtttctgga gggactgctg cttggggcga
     3241 ttgtcgcctc gacggatgcc gccgccgtct ttatggtgct gagggcgaga aatgtgaagt
     3301 tgccgcggcc gctggcgcat ctattggaat tggagtcagg cagcaacgac ccgatggccg
     3361 tggtgctgac ggtggccttg attcaactca tgaccaatcc gagcacgaac ttgtgggacc
     3421 tcctctggtt tttcgtcgtg caaatgacgg tcggggcggc ggccggtgtg gcgatgggcg
     3481 aactcatccg gtggacgctc aatacgttga aattggaatt acgcggcatc tacccggtgc
     3541 tgtcggtggc ctttgcgctc ttaacctatg ggctgaccgc cgaattgcat ggcagcggat
     3601 ttctcgcggt gtatctggcg ggactcttca tgcgacggca ctcggtcgtc caccagaaga
     3661 acctggtgca gtttcacgag ggtctggcct ggctgatgca aatcacgatg ttcctgatac
     3721 tcggattgca agtctttccc gcccgcctga ttcccattgc gggcgtcggt ctcctcgtgt
     3781 cccttttcct gattttcatc gcccgtccgg tcagcgtata cacgtcgctg gcattgtcca
     3841 ggctctcgtt ccgcgagcaa acgctggtgg catgggtagg tctgcggggt gcggtgccga
     3901 tcgtcttggc gacgtttcca gtcctggccg gcatccaaaa ggccgacacg atctttcacc
     3961 tggttttttt catcgccctg acgtccgttt tactgcaagg gccgccgata ccttggatga
     4021 cgcggctgtt ggggcttgat cgagtgctac aagaggggcg acgatgattg ccggtcaggt
     4081 ggcgtctgca ggcttggtcc aatgcttgaa gaacggtgcg atggtgtcga ttggaatcgg
     4141 aaagattgtg gtggagtttt tctcagcggc gatctccact agcgtctgga ggtagcggag
     4201 ttgcaaagcc gcaggattct ggctgatgac gttggccgca tcggccaacc gttgtgaggc
     4261 ctcgaattca ccttccgcat ggatgacctt cgcccggcgc tctcgttccg cttcggcttg
     4321 gcgggcaatt gcccgctgca tttcctgcgg caggtccacg ttcttcactt ccacagcggt
     4381 gactttcacg ccccatggct ctgtctgctg gtcgataatc cgctgcagat cggcattgat
     4441 ctgctcccgc ttggacagca ggtcgtcgag ctggctctgc cctaacacgc tgcgcagcgt
     4501 cgtctgcgac atcattgacg tggcgtacag atagtcttcg acttggatga tggctcgctc
     4561 ctgatctacg acacgaaagt agatgacggc gttgaccttg acggtgacgt tgtccttcgt
     4621 aataacgtcc tgcggcggga catccatggt gacggtccgc aaactgacgc ggaccagctt
     4681 gtcgatgaac gggatgataa tgacgacgcc tggaccattc ccgcccacga gcccgcgggc
     4741 cagacggccc cagcggaaga tgacg
//
LOCUS       DASSCQ010000165         4436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1521714, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000165 DASSCQ010000000
VERSION     DASSCQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521714"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..700)
                     /locus_tag="VFH05_11365"
     CDS             complement(251..700)
                     /locus_tag="VFH05_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678862.1"
                     /translation="MPTLTRTGQVKDVVIRDVVEPTSLAVNPGDEVRWINKRQGAVRV
                     IFLNPMTENLNCVRNFGGPLGFGTKRNEYSAKLGSNETASVCFRDSGEVKYVVRAESA
                     SDPSGEQNIAGTISIGSEDQASRPVAKPVEKNEGLAQSDKDEAASSR"
     gene            complement(1071..1544)
                     /locus_tag="VFH05_11370"
     CDS             complement(1071..1544)
                     /locus_tag="VFH05_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678863.1"
                     /translation="MMRKRMSRPPQEGAEAVCNWLNVEELAEVEITSEDAAHPVESAL
                     VAGSTSGWRAAEPGDQTIRLLFNHPQPLKRIWINFVELETERTQEYVLRWSSDDGQSF
                     REIVRQQWNFSPPNTTNEIEDHRVELPATKILELSIKPDISRGTAVASLACLRLA"
     gene            1891..2949
                     /locus_tag="VFH05_11375"
     CDS             1891..2949
                     /locus_tag="VFH05_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6678864.1"
                     /translation="MTVLLIALLTCFSHGQPEAIVGSGVSVDPSIEAYIAHAQVRGRM
                     TIVGSEATKPLLLKLAMEFRSRQPEATIAVQGPRGSKKSPVDAFLSGKSRMRRGDGDT
                     AGHLGSYDVELLASPRALTEQEFKRFTSKHGHPPTGFVIAYGALALYVNRENAIKSLT
                     LEQIDGIFSSTHKRGMADMRTWGQVGLAGAWVDAPIHMYGPHDRLSGTRSFFKEAMRL
                     NGNFKRSLKTEPGFASVIVAIGKDRYGIGYSLLGLETSAVRAVSVSENSGRPAVAPTA
                     ASVMSGEYPLSHPFYLYINQHPDKEWDPEVHEFLRFVNSRDGQDIVARTGLFPLSASQ
                     VSAHHTMLRARGIRGGPP"
     gene            complement(3039..3851)
                     /locus_tag="VFH05_11380"
     CDS             complement(3039..3851)
                     /locus_tag="VFH05_11380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6678865.1"
                     /translation="MPSWPLPQHDPWSTPFAESLLKHLDLFPGATILDVASGHGIPAF
                     YLAEQVGPTGQVVGIDLNAHQVRSARMIQGQQLPWLRFECLDMRAIPSNLPSADRITG
                     NLSVMFFRPDRFATLQNLSAHLKSGGQMVLTFPSLGTFDSLWKRVDQEMAARRLSEER
                     RRLEAYVAERPSAEQGREWLSKLNFERIIVREYPLEVQAGRGRAFLQDPLLRGGFLDD
                     VYECFDDATLAHEIMTAVSEDVASFMPLLAMRCVLSGWKPSGRAGAEVSNTS"
     gene            complement(4020..>4436)
                     /locus_tag="VFH05_11385"
     CDS             complement(4020..>4436)
                     /locus_tag="VFH05_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678866.1"
                     /translation="VFLFSKVNSWHTIRSAMRRETFADIWGSVWHNHVLASGEIRESM
                     MYGRTKLMGSAMGAVVFSVALSACRPVPIATAPPKSDPVVDLSGKVQSLQKQVQERDK
                     RIEELEAQLDALKLIDQDREKQRKPLRAPTSVEPFQ"
BASE COUNT          992 a         1255 c         1209 g          980 t
ORIGIN      
        1 ggacgagagt aagtggaatg agaagagagt tcgcgaccca cggcgaacga ccgtgatcgc
       61 gcttcgattc gatgtcggcc cgacgatcag ttcgttgaga ggtttctgag gtttctaatg
      121 actgcctcgt cgtcacccgc attccgtttt gtcgtgtcga aaaccaagac aggttgtctc
      181 cgcagacctt tttctcagtg ctccttggcg tccatccact gtgatggacg ccaagaattc
      241 aatcacgctg ctaccggctc gaagcggcct cgtccttatc agactgagcc aggccctcat
      301 tcttctcgac cggcttggcc accggtcggc tggcctggtc ttcggaaccg atagagattg
      361 tcccggctat attctgttct ccgcttgggt cactggccga ttcggccctg accacgtatt
      421 tcacctcgcc ggaatcccgg aaacacacgc tggccgtctc gttcgagccg agtttggccg
      481 aatactcgtt gcgctttgtt ccgaatccca acggtccccc gaaattgcgc acgcaattaa
      541 ggttctctgt catgggattc aagaatatga ctcggacagc gccctggcgc ttgttgatcc
      601 agcggacctc gtcaccgggg tttacggcca gggatgtcgg ttcgacgaca tctcggataa
      661 cgacatcctt aacttggccc gtacgagtca aggttggcat gctttcccca ccgatgctct
      721 ggcagccggt aagtaggact agagccgacc cgaggatgat gcggtggacg tgcattggta
      781 atccctcctt ttttgttgaa ggtgaaacat ctctcgccat ctcaaaaaga tgggatgaag
      841 ccacccatgg tgaagcatct gcaaaaggcg caccgccatc gtttcgctag ttctatatat
      901 atgtatgccc cagtttgtga cgacttcgcc ttagcttgga agacaacttg ttctcgtgag
      961 atgacaactt gcacgcaata tccagcatcc ttcaacacgg tgcagaggat ccccaacgca
     1021 tgtgtcccgg gggtggcgag gggcgtaaga aaaaggacga cgactgttcg ctaggcgagt
     1081 ctcaggcacg cgagcgaagc gactgctgtt ccacggctga tgtctggctt gatgctcagc
     1141 tccaggattt ttgttgcagg cagttcaaca cgatggtctt ctatttcgtt ggtcgtgttt
     1201 ggaggactga aattccactg ttgccgaaca atttcacgga aagactgccc atcatccgat
     1261 gaccagcgca ggacatattc ttgtgtacgt tcggtttcca gctctacgaa attgatccag
     1321 attcgtttga gcggttgcgg gtgattgaac agcagccgga ttgtctgatc accgggttct
     1381 gcggcccgcc atcctgaagt gctgccggcg accaaggccg actcgaccgg atgagcggca
     1441 tcctcagaag tgatttccac ctccgcgagt tcctccacgt ttaaccaatt gcacacagcc
     1501 tcagcgcctt cttggggcgg acgactcatt cgtttacgca tcatgtgcct cttgaacgtc
     1561 gctcaggctt gttggggaga cggagaaaca atgaccatcc tagtcacggg accattcccg
     1621 ggatactcgg agaaccccta cttgaatgat ttgccacgag aagcatgggc cacctctgaa
     1681 caatcacatc aacggcgact tcccctatac actgtatagc gtatagccta gcgagccgta
     1741 cgccatgtcc acactggacc tggaacacgg gagcgcagga aggcgctttg attgcattac
     1801 attagcactt atccctgaaa atgctatatc cactccctca gaattgagat gacgaataga
     1861 agaaacatac attccgcctt cagaacggtg atgaccgtgc tcctgattgc tctgttaact
     1921 tgtttttcac acggtcaacc ggaagctatc gtcggatcgg gggtctcggt cgatccctca
     1981 atagaggcct acatcgcgca tgcacaggta cgtgggcgca tgacaattgt cgggtcggag
     2041 gcgacgaaac cgctgctgct gaaattagct atggaatttc ggtcacgaca gcccgaggcg
     2101 acgattgcag tgcaaggccc tcggggatct aagaaaagcc ctgtggatgc gttcctcagc
     2161 ggcaaatcca ggatgcgccg aggtgacggt gatactgcag gccacctcgg atcctatgat
     2221 gtggaactct tggcgtctcc ccgcgcactg actgagcaag aattcaagcg attcacctcg
     2281 aaacatgggc atccgccgac aggatttgtc atcgcctacg gggcgctcgc cctctatgtg
     2341 aatcgagaga atgcaatcaa gagtctgacg ttagagcaaa tcgacgggat attttcctcg
     2401 actcataagc gcggcatggc ggacatgaga acatggggtc aagtcgggtt ggcaggcgct
     2461 tgggtcgacg ctccaattca catgtacggg cctcacgatc ggttgagtgg cacgagatcg
     2521 ttcttcaagg aagcgatgcg gctcaatggc aattttaaac gttccttaaa aaccgaaccc
     2581 ggttttgcat ccgtcatagt agccatcgga aaagaccgct atggcattgg ctatagtctc
     2641 ctagggcttg aaacatcggc agtcagagcg gtctcagtca gcgagaacag cgggagacca
     2701 gcagttgcac cgaccgctgc atcggttatg tcgggagagt atccgcttag ccacccgttc
     2761 tacttgtaca tcaatcagca tccggacaag gagtgggatc ccgaggtaca cgaatttctg
     2821 aggttcgtga acagtcgcga cgggcaggac atcgtggccc gcacaggcct cttccctctt
     2881 tccgcgtcgc aggtgagtgc gcatcacacc atgttgcgtg caaggggaat acgaggcggt
     2941 ccaccttaga gaaccttcca caattcgtcg cagtcgccgg tcggcacgtt caccggcagg
     3001 ttgccaggca caaagcaagg caaagtgaca cacgagggtc agctcgtgtt gctgacttcc
     3061 gcacccgccc ttccagaggg cttccatccg gatagcacgc accgcatcgc tagcagaggc
     3121 ataaagctcg ctacgtcctc tgacaccgcc gtcatgatct catgggcaag tgtggcgtcg
     3181 tcaaagcact catacacatc atcgaggaac ccgccacgca acagcgggtc ttgcaggaat
     3241 gcgcgtccgc ggccagcctg gacttccaag ggatattcac gaacgatgat gcgttcgaag
     3301 ttgagcttgg acagccactc acggccttgt tcggcagatg gtcgctcagc gacataggct
     3361 tcaagccgtc gtcgttcctc actcaagcgc cgtgctgcca tttcttgatc cacccttttc
     3421 cagagggaat cgaacgtgcc cagcgacgga aacgtcaaga ccatttgtcc cccagatttg
     3481 aggtgtgctg atagattttg tagggtcgca aaccgatccg gccgaaagaa catcaccgac
     3541 aaattaccgg tgatccgatc agcacttggc agattcgagg ggatcgcgcg catatcaaga
     3601 cactcgaacc gtaaccatgg gagctgctgc ccctgaatca tcctcgcgct cctcacctga
     3661 tgcgcgttca gatcgatacc cactacctgc ccggttggac cgacctgctc ggcaagatag
     3721 aacgcaggaa tgccatgccc cgaagccacg tccagaatcg ttgcccctgg aaaaagatca
     3781 agatgcttga gcaacgattc ggcaaacggc gtcgaccagg gatcatgttg gggaagaggc
     3841 caggacggca cggcagcctt tcaatgtcta acgactctcc acagacggta cagtctgagg
     3901 gcgcgcgctc aagacggctg gcaatgcggc tccctcacgg agagcgtctg tgacattctt
     3961 agaaggacga gaaatgggag cgttgaattg cacgtcccgt cgccacggta gaacgcccgt
     4021 tactgaaacg gttccaccga cgtgggcgcc cgaaggggct ttcgctgctt ttcgcggtct
     4081 tggtcaatga gcttgagtgc gtcgagttgg gcctccaact cctcaatacg tttatcccgc
     4141 tcttgaacct gtttctggag agattgcacc ttgcctgaca gatcgacaac gggatcgctc
     4201 ttgggcggtg ccgtggcgat gggaaccggt cgacacgcgc tcagagcgac ggaaaacacc
     4261 accgccccca tcgcgctgcc catgagcttc gtccgcccgt acatcatgct ctctcggatc
     4321 tcccctgacg ctaatacatg attgtgccag actgagcccc agatgtcagc aaaggtttcg
     4381 cggcgcatgg cggacctgat ggtatgccat gagttgactt tgctgaagag aaatac
//
LOCUS       DASSCQ010000166         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1063706, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000166 DASSCQ010000000
VERSION     DASSCQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063706"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFH05_11390"
     CDS             complement(<1..188)
                     /locus_tag="VFH05_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET6678867.1"
                     /translation="MSAPSAERFDVALNTPAGRLNTVLDVPTGFVPITSIIPLTRRLG
                     EEMLKLEEHQSREAGHTIS"
     gene            303..668
                     /locus_tag="VFH05_11395"
     CDS             303..668
                     /locus_tag="VFH05_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678868.1"
                     /translation="MALSGHVVGLLKEYMHDLVIQAEQEAAAHQSFGFSQTSYRSDQA
                     ISDLLAILDDRIESEGVQVGLPEDVLHEMWSLCNEARDHLTDQVWLDRNVSDQPASKA
                     KVRELTYHALIKFLEAKQR"
     gene            707..1861
                     /locus_tag="VFH05_11400"
     CDS             707..1861
                     /locus_tag="VFH05_11400"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6678869.1"
                     /translation="MWPLLLLLALGALTGVASTAGRPPEAGLELVPIVTHGLQLPLFA
                     THGADGSGHLFVLEQPGLIRVIDHGVLREAPFLDLRDRVWTKGNEQGLLGLAFHPEHR
                     GNGRFFVNYNRREDGATVVAEFTRNGRSLDVSAETERILLVVPQPYLNHNGGMMAFGP
                     DGMLYIGRGDGGSKGDPQNRAQNPQELLGKILRIDVNRGQPYAVPRDNPFVTGKGRPE
                     VFALGVRNPWRFSFDRETGLLWLADVGQYKWEEIDIVVAGGNYGWRIMEGAHCYNPEE
                     GCSPDGLMFPIAEYGHEEGRCSITGGYVYRGPSVQALRGTYVFGDYCSGELFGISADA
                     NRKGSTVPRVLMRTGLRISSFGEDQAGEIYIVDHRGGVYRLAASRASRTR"
     gene            complement(1889..2701)
                     /locus_tag="VFH05_11405"
     CDS             complement(1889..2701)
                     /locus_tag="VFH05_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET6678870.1"
                     /translation="MELTPFLFGLYKLVKYLLYPLTWVLLLLTMTTVLLWLPPSHRRL
                     QWARIWAVSALMLLVAISTPLVARPLLGTLEAWYPRPSLSSQEQYHAVVVLGGGVLQR
                     GTLRPMPDLSAFSKNRTACGVDLFKQGYADKLVFTGGSSHVFKEGPRDALEMKRWAER
                     LGVPAHAIVTEEQSRTTYENATETKRLLGPASILLVTSASHLPRAVALFTKQGFRVTP
                     VPCDFLMQNRPEDSWNQLDLFDVLPNEHAIEHTTVAVTEWAGMAMYWLNGKL"
     gene            2762..3244
                     /locus_tag="VFH05_11410"
     CDS             2762..3244
                     /locus_tag="VFH05_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET6678871.1"
                     /translation="MTRSPRYWLVKSEPTAFSIDDLARAPRQTTCWDGVRNYQARNFL
                     REMAIGDQVLFYHSNAEPPAVVGRAEVVKTAYPDDTQFDRRSHHFDPASTRERPRWDM
                     VDLKYKGKFHTALPLDRLRQEPQLKEMVLLRKGSRLSVQPVTGREWSVIMRLAGSGKG
                     "
     gene            complement(3326..3571)
                     /locus_tag="VFH05_11415"
     CDS             complement(3326..3571)
                     /locus_tag="VFH05_11415"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HET6678872.1"
                     /translation="MRIPSRVVLPFGYRISVRQLSDTEMDRRDPNADGIWDDELKTIY
                     LRKRLPVTRRRYILAHELGHAWLDWQHRHLDNGKAKT"
     gene            3747..>3978
                     /locus_tag="VFH05_11420"
     CDS             3747..>3978
                     /locus_tag="VFH05_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678873.1"
                     /translation="MIVVRRPFGIGDCFAAIARMLLMGFLAGPSLALAVPHAPHGMVT
                     VDGVTVPEIGPMPTQVPTPPTNLSYSEKVELGK"
BASE COUNT          832 a         1101 c         1200 g          845 t
ORIGIN      
        1 gaaatcgtgt gtcctgcctc gcgcgactga tgctcctcca gtttcagcat ctcttcgccg
       61 agacgccgcg tgagcgggat gattgacgta attggaacga accccgtggg gacgtccaac
      121 accgtattga gacggccggc aggggtgttc agcgcaacgt caaagcgttc ggcggagggt
      181 gctgacatcg gtgcatcata gaaaaccgcc aaccggttgg tcaattgaag actggccggt
      241 gcatgcgtct aggttgctct cccttccgct tcccccgcat aatgcgccgg aggagaaggg
      301 gcatggcgct gtcgggccac gtcgtgggcc ttttgaagga atacatgcac gatctggtca
      361 tccaggccga gcaggaggcg gcagcgcacc agtcatttgg gttttcgcag acatcctatc
      421 gatcggacca ggccatctcc gatttgctgg ccatcctcga cgatcgcatc gaatctgaag
      481 gcgtccaggt cggattgccg gaagatgtgt tgcatgagat gtggagcctg tgcaacgagg
      541 cgcgggatca cctgacggac caggtatggc tcgatcgaaa tgtgagtgac caaccggcgt
      601 cgaaggccaa ggtccgtgaa ttaacctatc acgcactcat caagtttctg gaggctaaac
      661 agcgatgagg ggatgcctgc acccgccccc cttcccggtg ttccgtgtgt ggcctctcct
      721 gttgctactt gcattaggcg cgctcacagg tgtggcgtct acggcgggcc gaccccctga
      781 agccgggctc gagctcgttc ccattgtgac gcatggctta cagttgccat tgtttgcgac
      841 gcatggggca gatggttcgg ggcacctgtt tgtcctcgaa cagcccggcc tgatccgggt
      901 catcgatcac ggggttctca gggaagcgcc cttcctggat ctacgcgacc gtgtgtggac
      961 aaaaggcaat gagcagggcc tcttaggctt ggcttttcat cctgagcatc gcggcaacgg
     1021 ccggttcttc gtgaactaca accggcgtga ggacggtgcg acggttgtgg cagagtttac
     1081 tcgcaatgga cgcagtttgg acgtgtcggc cgagaccgaa cgcattctcc tggtcgtgcc
     1141 gcaaccctat ctcaaccata acggaggaat gatggcgttc gggcctgatg ggatgctgta
     1201 catcggtcgc ggagacgggg gctcgaaagg cgatccgcaa aatcgggctc agaaccctca
     1261 ggagttgctg gggaagatcc tgcggatcga cgtgaatcgg ggccagccct atgcggtccc
     1321 tcgggataat ccctttgtca ctggaaaggg tcgaccggag gtgtttgcct tgggagtcag
     1381 gaacccctgg cgattttcat ttgatcggga aaccggcctg ctctggctgg ccgatgtcgg
     1441 gcagtacaaa tgggaagaga tcgatatcgt ggtcgccggc ggcaattacg ggtggcgcat
     1501 catggaaggc gcacactgct acaacccgga ggaaggctgt agcccagatg gcctcatgtt
     1561 ccccatcgcg gagtatggac acgaggaggg tcgctgttcg atcaccggcg gctatgtcta
     1621 ccgcgggcca tccgtccagg ccttgcgggg gacctatgtc tttggtgatt actgtagcgg
     1681 agaactgttc ggcatctccg ccgatgcaaa tcgcaaaggc tcgaccgtcc cccgagtgct
     1741 gatgcgaacg ggattgcgca tttcttcttt tggcgaagac caggccggag aaatctatat
     1801 cgtggaccat cgaggtgggg tctaccgact cgccgcttca cgagcatcgc gtacccgcta
     1861 ggctcatctg cccgttctgc aactgtggct agagtttccc gttgagccaa tacatcgcca
     1921 tccccgccca ctctgtgaca gccaccgtcg tatgttcgat cgcatgctcg ttgggaagga
     1981 catcgaagag gtcgagttga ttccaactgt cttcgggacg gttttgcatc agaaagtcgc
     2041 aaggaaccgg tgtgacgcga aatccttgct tcgtaaagag ggcgacggcg cggggcagat
     2101 ggctggccga ggtgaccagc aaaatggatg caggtcccag gagccgcttg gtctcagtgg
     2161 cgttctcata agtcgtacgc gattgttctt ccgtgacgat ggcatgagcg ggtactccca
     2221 gccgctcggc ccagcgtttc atttcgagag cgtccctcgg cccttccttg aacacatggc
     2281 tactgccgcc agtgaagacg agcttgtcgg cgtacccttg tttgaacagg tccacgccgc
     2341 acgccgtccg gttcttagag aaagcagaga gatccggcat ggggcggagg gtacctcgtt
     2401 gaagcacccc tcctccaagg accacgactg catgatactg ttcctggctt gataaggatg
     2461 gccgtggata ccaggcctcc aaggtgccga gtaagggacg agccacaaga ggagtggaaa
     2521 ttgccaccag taacatcagc gcgctgactg cccagatccg cgcccattga agccggcgat
     2581 ggcttggcgg caaccacaac aatacggtcg tcatcgtcaa caagagcaag acccacgtca
     2641 gcgggtatag aaggtacttg acgagcttat acagcccaaa caagaatggc gtgagttcca
     2701 ttgcagcgcc tcatagagaa atgtctttag gaacgtttat cacacgctcg tcgccgttta
     2761 catgacacgt tcgcctcgtt actggctggt caagtccgaa cccacggctt tttccattga
     2821 tgatctggca cgcgccccgc gacagacgac gtgttgggat ggagtccgga attaccaagc
     2881 gagaaacttt ctgcgcgaaa tggccatcgg cgaccaggtg ctgttttacc acagcaacgc
     2941 ggagcctcca gccgttgtcg gccgtgccga ggtggtaaaa acggcttatc cagacgatac
     3001 acaattcgac cggcggagcc accactttga tcccgcaagc accagggagc ggccccgttg
     3061 ggatatggtc gatctgaaat ataaaggaaa atttcacacg gccttgcccc ttgatcggct
     3121 caggcaagaa cctcaactca aagagatggt gctgcttcga aaggggtcac gcttgtctgt
     3181 tcaaccggta acggggcgtg aatggtcggt cattatgaga ttagccggca gtgggaaagg
     3241 atagctacca gactgtgacc gcaaggcaat aggaagttgc gtacaagtca gtagaacggc
     3301 gagcctcacc gttggccctc gtgggtcatg tcttggcctt cccgttgtcg agatgcctgt
     3361 gctgccagtc cagccaggca tggccaagtt catgcgcgag gatgtagcgg cggcgggtga
     3421 cggggagccg ttttctcagg taaatcgttt tcaactcgtc gtcccagatg ccatcggcgt
     3481 tggggtcgcg acggtccatc tcggtgtccg aaagctgccg gacagagatg cgatacccga
     3541 acggcaagac cacacgtgaa gggatgcgca tgaggtgccc caattgttta cgaagatagc
     3601 ctaccacaag tggcttttgg ccttcatgcc gcaatattca tataaatcaa cagtttattc
     3661 gatagatggc cctccaagac cgtggtcaat ccaaacatgg tactgttggc tcggcagtct
     3721 ccaacatttc aaaaatagga ggcactatga tcgttgtgcg acgtcccttt gggatcggtg
     3781 attgcttcgc ggccattgcg cggatgctgt tgatggggtt cctagcgggc ccttccctcg
     3841 ccctggctgt tccacatgca ccgcacggca tggtcaccgt cgatggcgtc accgtgcccg
     3901 aaatcgggcc tatgccgacg caagtgccga cgcctcccac gaatctgagt tactccgaga
     3961 aagtcgagtt gggcaagc
//
LOCUS       DASSCQ010000167         4260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1178494, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000167 DASSCQ010000000
VERSION     DASSCQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178494"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFH05_11425"
     CDS             complement(<1..462)
                     /locus_tag="VFH05_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678874.1"
                     /translation="MATSSTMRGSIPAMASQSEWHMTLGAQVDTGGVRFRVWAPKRAR
                     VDVVLEEGGRSFPLMKDEAGYFSGLVPIATAGMLYRYRLDNGDAYPDPCSRYQPQGPH
                     GPSLIVDPSTYAWRHPEWQGVRMQGQVIYELHVGTFTPEGTLDATIAQLDEL"
     gene            799..1272
                     /locus_tag="VFH05_11430"
     CDS             799..1272
                     /locus_tag="VFH05_11430"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HET6678875.1"
                     /translation="MRNQNWYTMLITGLCMIASGCITDATTELTKAPFDATSQLTNGT
                     TAALSEFTDPLTDFTSSTTPGAEGGIVPSRAKKRLQIFTASSYENLRADISRGHGEYL
                     VSLATLAGVPSGQVRQFQTFMQDSYSTMFEEEIPIPESTHQIVEIAWAAGYGQRN"
     gene            complement(1347..3530)
                     /locus_tag="VFH05_11435"
     CDS             complement(1347..3530)
                     /locus_tag="VFH05_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678876.1"
                     /translation="MSNPISVVEINYEDDLVTARQRARQVARLLTFEEQDQTRISTAV
                     SEIARLFVHSKRPALIEFQIEGTTVPQLLLVDIGWPRGGSAKSGSTLPSNSRTFDPEW
                     ESAVTSARRLMDHGEVRSESPERSLIRLVKILPARAPLFVGKALERVIKQLGKQEPQN
                     PMEEVQQQNQELLRALEELHERQQELVRLNGELEDTNRGVVALYAELDEKAGHLRRAD
                     EMKSRFLSNMSHEFRTPVNAILALSHLLLERTDGELNSEQEKQVGYIRKSGEDLLELV
                     NDLLDLAKIEAGKITVKASEFEVANLFSALRGMLRPLLVTDAVNLVFEDPSGIPLMTT
                     DEGKVSQILRNFVSNAIKFTPRGEVRVTAALADDGRAVAFSVTDTGIGIASEDQSRIF
                     EEFSQVDHPLQLQVKGTGLGLPLCRKLASILGGSITVTSRLGVGSTFVATIPIQYSVN
                     TIEVAEQIEPVQVRADDFRLPILVVEDEPETRLLYDKYLRNTAFRPVAAGSIRQAREQ
                     LRRQSFAAIVLDVLLPDESAWQWLAELKSQEQTKKLPVLIVSSVEDPRKAMALGADDY
                     CVKPLRRIWLLDRLHRVTGIVREPSPGPPLVLVIDDQEADRYILRHYLEDFRCSIVEA
                     TGGEEGLKLARQLKPALILLDLNMPKIDGFEVLAQLKANPDTASLLVVIVTSHVLAPE
                     QSTTLAHARAVLMKHELSPAAWQKLFQEIGLTGIELSVPETLKPS"
     gene            complement(3509..>4260)
                     /locus_tag="VFH05_11440"
     CDS             complement(3509..>4260)
                     /locus_tag="VFH05_11440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6678877.1"
                     /translation="GLGAVLRTAGEFDVYSQPGKGTAIVARVKGRQNGREEPQAQAVG
                     VVQQAKPGETVCGDNWIVRWSADGWVCAIADGLGHGVIASQAATAIVEAVRRAPANRT
                     PVELVEAAHQAAKPTRGAAVGIAVLDRPKGLLRFAGIGNIAALVVNGSERRHLVSHNG
                     IIGHDYRKLAEFSQPWHAHSLLLLHSDGIATHWDLDRYPGLLSRDPSLIAGILYRDFK
                     RGRDDATVVVVKQRLSAGVPSPESPCPTRSV"
BASE COUNT          855 a         1293 c         1219 g          893 t
ORIGIN      
        1 cagttcgtcc aattgtgcga tcgtcgcgtc gagcgtgcct tccggtgtga aggtacccac
       61 atgcagttcg tagatgactt gaccttgcat ccgcacgccc tgccattcgg ggtgacgcca
      121 ggcgtaggtg gacggatcaa caatcagcga tggaccgtgc ggtccttggg gttgatagcg
      181 ggaacaggga tcaggatagg cgtcaccgtt atccagccgg tagcggtaga gcatgccggc
      241 ggttgcgatg gggacgaggc cggaaaagta ccccgcctcg tctttcatta atggaaaact
      301 ccgtccgccc tcttccagaa cgacatccac tcgcgcccgc ttgggcgccc atacacggaa
      361 ccggacgccc cccgtatcca cctgggcgcc caacgtcatg tgccactcag actgacttgc
      421 catcgcgggg atactacccc gcatggtcga gcttgttgcc atacatcctc atcctgtgag
      481 ccatgggcga agagtagccg cggaatgcaa cattccttac ccgtttcaca atattcatat
      541 ttatccgatt gttcagtttg tagcattctg gaacgggaag gacagcctgg gaaggaccct
      601 agtatagagg ctcctcccga tgagtgaaca tccacacgag caaaagggct ggatgcgctg
      661 acgcagagcg cgtacttaca ccactatcag tgagagaaaa tgatgcgcct atgggtcttc
      721 ctaggcgcgg gtcttcacgc aacgacgtat tcatatttat agggaaagca gttggcattc
      781 tcgcgatgag gaggacccat gaggaaccag aattggtaca cgatgctgat aaccggcctg
      841 tgtatgattg cctctggttg cattaccgac gcaacgacgg aactcaccaa ggcgcctttc
      901 gacgccacat cccagctgac caacggcacg accgcggcct tatccgagtt cacggatcca
      961 ttgacggatt ttacctcgag cacgacgccc ggcgcggaag gagggatcgt tccgtcacga
     1021 gcgaagaaac ggttgcagat cttcacggcg tcttcctatg aaaacctccg cgcagatatt
     1081 tcgcgcggtc atggggagta cctggtgtcg ctcgccacac tggccggtgt cccgtcggga
     1141 caagtgaggc agttccaaac cttcatgcag gattcttaca gcacgatgtt cgaagaggaa
     1201 attccgattc ccgagtcgac ccaccagatc gtggagatcg cctgggcagc tggatatggt
     1261 cagcggaact gacgatgccg gcggtgagag ctgttcacgg aagcggcgcc acgtgcagtc
     1321 tctgtttgtc ggtggcggga tcgacgtcag gaaggtttta gcgtttcagg aacggagagc
     1381 tcgataccgg ttagtcctat ctcctggaac agtttctgcc aggcagccgg cgacagttcg
     1441 tgtttcatca gcaccgctcg agcatgcgcg agcgtcgtgc tctgttcagg cgctaataca
     1501 tgagaggtga cgatgaccac gaggagtgac gcggtgtccg gattcgcctt cagttgtgcc
     1561 agcacctcaa agccgtcgat cttcggcatg ttgagatcca acagaatcaa cgccggcttc
     1621 aattggcgag ccagcttcag tccctcctcc cctccggttg cctcaacaat ggaacatcga
     1681 aaatcttcga gatagtgtcg gagaatgtac cggtccgctt cctgatcgtc aatgacgaga
     1741 accagcggtg gacccggcga cggttcccgt acgatgccgg tgacacgatg caggcggtcc
     1801 agcagccaaa tgcgtcgcag cggcttgacg cagtagtcat ccgctccgag cgccatcgcc
     1861 tttcgcggat cctcgaccga ggagacaatg agcaccggaa gcttcttcgt ctgttcctga
     1921 cttttgagct cggcaagcca ttgccacgct gactcatcgg ggagaagcac atccagcacg
     1981 atggcggcga aagactggcg gcgcagttgt tcgcgggcct ggcgtatgct gcctgcggcg
     2041 accggccgaa atgctgtatt gcggagatat ttgtcgtata ggagacgggt ctcgggctca
     2101 tcttcgacca ccaagatcgg caggcggaag tcgtccgccc gtacttgcac cggctcgatc
     2161 tgctccgcca cttcaatcgt gttgacggaa tattggatgg gaatcgtggc cacgaaggtg
     2221 gatccgacac ctaatcggct ggtcacggtg atggacccgc ccaaaatgct ggccagttta
     2281 cgacacaggg gcaatcccag accggtccct ttcacctgta gctgcaatgg atgatcgact
     2341 tgtgaaaatt cttcgaagat gcggctctgg tcttccgagg ctatgccgat tcccgtatcc
     2401 gtcactgaaa aggcgaccgc acgtccgtca tcggccagcg ccgccgtcac gcgcacttcg
     2461 ccccgcggcg taaatttgat cgcattcgac acaaaattac gcaggatttg agacaccttg
     2521 ccctcgtcgg tggtcatgag cgggatcccg gaggggtctt cgaacaccag attcacggcg
     2581 tcggtcacca gcaacggacg tagcatgcct ctcagggcgc tgaacagatt ggccacttcg
     2641 aattccgagg ctttgacggt gatttttccg gcttcaatct tggcgaggtc caggagatca
     2701 ttgaccagtt caagcagatc ttcgccgctc ttccggatgt agccgacctg tttctcctgc
     2761 tcgctgttta actccccgtc cgtacgctcg agcagcaagt gggacaaggc gagaatcgcg
     2821 ttgacgggcg tcctgaactc atggctcata ttcgacagga agcgtgattt catttcgtcg
     2881 gcccgccgca agtgacccgc cttttcatcc agctctgcgt agagggccac cacgccccgg
     2941 ttggtgtctt ccaactcgcc gttgagcctg accagctcct gctgtcgttc atgcagctct
     3001 tcgagcgccc gcagcaattc ctgattttgc tgctgcactt cctccatcgg gttttgcggc
     3061 tcttgcttgc cgagttgctt gatgacccgc tccaacgcct tccctacgaa cagcggagcg
     3121 cgggccggca gaattttcac gaggcggatg agactcctct ccggcgactc cgaccggact
     3181 tccccatgat ccatcaaacg tcgcgcggag gtcaccgccg actcccactc gggatcgaag
     3241 gttcggctgt ttgacggcag cgtcgaaccg gacttcgcgg atccgccgcg gggccatcca
     3301 atgtccacaa ggagcagttg cggcacggtc gtcccttcga tctgaaattc aatcagggcc
     3361 ggtcgttttg aatgcacgaa caatcgagca atttccgaca cggccgtcga gatacgcgtt
     3421 tggtcctgtt cctcaaacgt cagcagccgt gccacctgcc gcgcccgttg ccgtgcggtg
     3481 accaggtcgt cttcgtagtt gatctcgact acactgatcg ggttggacat ggtgactccg
     3541 gagatggcac gccggcggag agacgttgct tgaccacaac cacggtggca tcgtcccggc
     3601 ctcgcttgaa gtctctgtac aaaatgccgg cgatgaggct cggatcgcgt gacagaagcc
     3661 cggggtaacg gtccagatcc cagtgcgtag cgatcccgtc gctgtgcagc agcaacagcg
     3721 agtgcgcatg ccacggctgc gaaaactctg cgagcttgcg atagtcatgg ccgatgatgc
     3781 cgttatgcga tacgagatgc cgccgctccg acccgttcac caccaaggcc gcaatattgc
     3841 cgataccggc gaatcgcaac aggcccttgg gtctatccaa gacggcgatg ccgactgcag
     3901 cgccgcgcgt gggtttcgcg gcctgatggg cagcctccac taattcgacc ggcgtccggt
     3961 tggccggagc ccgacgcacg gcttcgacga tggccgtcgc ggcctgcgac gcaatcacgc
     4021 cgtggcccaa gccatcggct atggcgcata cccaaccgtc tgccgaccag cggacaatcc
     4081 aattatcgcc gcacaccgtt tctccgggct tggcttgttg gaccactcct acggcctgtg
     4141 cttgcggctc ctcacgccca ttctggcggc ctttcactct cgccacaatg gcggttccct
     4201 ttcccggctg tgagtagaca tcaaactccc cggccgttcg aaggacagcg cccaaccccg
//
LOCUS       DASSCQ010000168         5169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1159545, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000168 DASSCQ010000000
VERSION     DASSCQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159545"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..1685)
                     /locus_tag="VFH05_11445"
     CDS             complement(357..1685)
                     /locus_tag="VFH05_11445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET6678878.1"
                     /translation="MRNFLSREATVAKAGFNRWLVPPAAIAVHMCIGEVYGFSVFNVP
                     LTQAGAGWTIPQVGWIYSIALFMLGVSAAVFGKWVERSGPRKTMAASCACFCGGLLIS
                     ALGVKLNMLWLLYVGYGFIGGIGLGLGYISPVSTLIKWFPDRPGMSTGMAIMGFGGGA
                     LIGAPLGVKLMDHFTSATSVGVAEAFIAMACLYAVYMLFGAFVVRVPPQGWKPDGWEP
                     EKHLKPMMTNANVGVDLAWKTPQFWCLWVVLCMNVSAGIGILGQASKMCSDMFGVSAA
                     VGGGFAGLLSIFNMGGRFVWSSISDYTGRKTVYCIYFILGALLYCAVPWTQHIQHKAL
                     FVVVTAIIITMYGGGFATIPAYLRDMFGTYQVGAIHGRLITAWSIAALLGPNLVNYIS
                     TAKIEAGVPAAEAYNSTMFIMAGLLVIGLIANLLVRPVDPKYYLAESPKG"
     gene            complement(1776..2360)
                     /locus_tag="VFH05_11450"
     CDS             complement(1776..2360)
                     /locus_tag="VFH05_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678879.1"
                     /translation="MQSARVLIIEDDPVFLDTLSSTLRLKLPQAQVDTADSALTALER
                     IRSMDYAAILCDAHQPRLEGIGFVRAVHKLHPECSVLLLLEKQQEDLLRQAMHAGAYD
                     VLVKPVEEGTLLFAVRRAIEASQLRCQVKREEAQLVASVGSMMKDLEVLYGAYGLHAH
                     FEAFMASWEIEKGEERRAHPSCAPNVRPRDSSNL"
     gene            2579..4726
                     /gene="glgX"
                     /locus_tag="VFH05_11455"
     CDS             2579..4726
                     /gene="glgX"
                     /locus_tag="VFH05_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET6678880.1"
                     /translation="MRIWPGSPYPLGATWDGEGVNFALFSENATAVELCLFERPDDAR
                     ETHRIRIEECTDFVWHVYLPESRPGQLYGYRVHGLYEPEAGLRFNPAKVLIDPYAKAI
                     AGNIDWSDALFGYRIGDPKADLSLDDRENAGSIPKCVVVDQAFTWGGDQLLRTPWDRT
                     VIYEVHVHGFTARHPDVPPNLRGTYAGLATPEVIEHLQHVGVTAVELLPVHHFVRDKH
                     LVDRGLTNYWGYNSIGFFAPDIRYAASPERAHHVWEFKTMVKALHNAGIEVILDVVYN
                     PTGEGNHLGPTLSFRGIDNASYYRLVPDHPRFYQDYTGCGNTLNVRHPRVLQLIMDSL
                     RYWVLEMHVDGFRFDLASTLARELHDVDRLSAFFDIIHQDPVLSQVKLIAEPWDLGEG
                     GYQVGNFPAGWAEWNGRYRDAIRRYWKGDGGQVAELAYRLSGSSDLYEGGGRRPHASI
                     NFVTAHDGFTLHDLVSYNQKHNEANGEDNRDGTDDNLSWNCGVEGPTNKPSIAALRER
                     QKRNMLATLLLSQGVPMLCSGDEIGRTQGGNNNAYCQDNEISWVDWTLSKPQQALLTF
                     TRGLIALRQKHPVFRRRRFFQGRRIRGMEVKDLYWLRPDGKEMTDEDWTQGYVRCLGV
                     RLAGDAMEEKDAKGRPLLDETFLVLLNAHHEPRPFTLPAHKRGVRWQPVFDTASYDTN
                     GKPVTLFRGGDQYDLEARSLAVLRLRSKQPSAS"
BASE COUNT         1115 a         1554 c         1533 g          967 t
ORIGIN      
        1 cggtatagcc gtcaactttc agatcgttca gcaatcggcg tatttctccc tccaacgtat
       61 tgcggcagat gatggtcagc atcttcatgg cgtccgtctc atggtacacc attttttacg
      121 tgacgcagtc cataacagca aacttctctc ccgtctcact cgaaatccct gccgccgagc
      181 agtttcctgg ccgcccgaag caacaacatg actggctcgc ttggctgctc ggcacagcag
      241 gctctgattg tccctgaaaa agaggcatcg acttctgtac cgattgatac acgccccagc
      301 ccagcggcac ggcaatcaac atccaggcga tggcgagagc gatggttttc atacgtttag
      361 cccttgggcg attcagcgag gtagtatttc ggatccaccg ggcgcacgag gagattggcg
      421 atgaggccga tcaccaaaag gccggccatg atgaacatgg tggagttgta agcctcggct
      481 gcgggcactc cggcttcgat cttggcagtg gagatgtaat tgacgaggtt ggggccgagc
      541 agcgcggcga tggaccacgc ggtgatgagg cgtccgtgaa tggcgccgac ctggtatgtg
      601 ccgaacatgt cccgtaaata tgcggggatc gtggcaaagc cgccgccata catggtgatg
      661 atgatggccg tgacgacgac gaacagcgct ttgtgctgga tgtgctgcgt ccacgggacg
      721 gcgcaataca ggagcgcccc gaggatgaag tagatgcaat agaccgtctt gcgtcccgtg
      781 tagtcggaga tggatgacca cacgaagcgg ccccccatat tgaagatgct cagcaggccc
      841 gcgaagccgc caccgacggc cgcgctgacg ccgaacatgt cggaacacat ctttgatgcc
      901 tgtccgagaa tgccgatgcc ggcgctgacg ttcatgcaca gcacgaccca gagacaccag
      961 aactgcggtg tcttccacgc gaggtcgact ccgacgttgg cattggtcat catcggtttg
     1021 agatgcttct ccggctccca gccgtccggc ttccagccct gaggaggaac gcggacgacg
     1081 aatgcgccga acaacatgta gaccgcatac agacatgcca tggcgatgaa tgcctcggcg
     1141 accccgacgg aggtggctga tgtgaaatgg tccatcaatt tcacgccaag aggcgcgccg
     1201 atgagcgcgc cgccgccgaa tcccatgatc gccatccccg tggacatgcc gggccggtcc
     1261 gggaaccatt tgatgagcgt ggacacgggc gagatgtagc cgagtccaag gccgatgccg
     1321 ccgataaagc cgtacccgac gtacaggagc cacagcatgt tcagtttcac gccgagggcg
     1381 gaaatgagca ggccgccgca gaagcaggcg caactggctg ccatcgtctt gcgcggtccg
     1441 ctgcgttcca cccacttccc gaacaccgcc gccgacacgc cgagcatgaa gagtgcgatg
     1501 gagtaaatcc atccgacctg aggtatcgtc cagcccgcgc cagcctgggt caacggcaca
     1561 ttgaacacgc tgaacccata cacctcgcca atgcacatgt gcacggcgat ggcggcgggc
     1621 ggaaccagcc agcggttgaa tccggctttc gcgacggtgg cctcacggga gaggaaattc
     1681 ctcatcaaag ttccattggt ggaaagcggg aagggacggc tcatagccgt cctagatggc
     1741 ggcccgatta ttccgacagg gatccaacgt ctcgcttata gattcgatga gtcccgcggc
     1801 cgcacgttgg gagcgcagga gggatgggcg cgtcgttcct cccccttctc aatctcccat
     1861 gacgccatga atgcctcaaa gtgtgcgtgc aatccatagg cgccgtacaa cacttcgaga
     1921 tctttcatca tgcttcccac cgaagcgacg agctgcgcct cttctcgctt gacctggcat
     1981 cgcagttgag atgcttctat agcgcggcgg acggcgaaca ggagtgtgcc ctcctcgacc
     2041 ggcttcacca acacgtcgta ggcaccagcg tgcattgctt gccgcaggag atcctcctgt
     2101 tgtttttcga gcagaagcaa gaccgagcac tcgggatgaa gtttgtgtac cgcccggaca
     2161 aatccgatgc cttcgaggcg gggctgatgg gcatcgcaga ggatcgccgc atagtccatc
     2221 gaccggatcc gctccaatgc cgtcagtgcg ctgtctgctg tgtcgacctg agcctgtggg
     2281 agcttgagac gcaaggtcga cgacagggtg tcgaggaaca caggatcatc ttcaatgatg
     2341 aggacacgag cagactgcat ggtgtttccc ataattccca taataaagat gtggatgacc
     2401 gatgtccgcg tgtcgtgagg cagatgtgcc gtgacataaa ggcgtccggg aatggggcgg
     2461 agtcgagagc aacgcgatga ctctttcacc agcggacatg tgccattata tgataatttg
     2521 atccaagcgc ccggacaatt ttcacaatcc tcacggcacg caacgagagc gcagccctat
     2581 gagaatctgg cctggcagtc catatccctt gggagccacg tgggacggcg aaggagtgaa
     2641 cttcgcattg ttttcggaaa atgcgacagc cgtggagctt tgtctcttcg agaggccgga
     2701 cgacgcgcga gagacgcacc gcatacggat cgaagaatgc accgatttcg tctggcacgt
     2761 ctacctgccg gagagccggc ccggacaact ctatggatac cgggtccacg gcctgtatga
     2821 gccggaagcc ggcctccgat tcaatccggc caaggtcctg atcgatccgt atgccaaggc
     2881 gattgcgggc aatatcgatt ggtcagacgc gctcttcggc taccggatcg gtgatcccaa
     2941 ggccgatctc tccctcgacg atcgggagaa cgccggctcg atacccaaat gtgtggtcgt
     3001 cgaccaggcg ttcacctggg gcggcgacca actgttgcgg acgccctggg atcggaccgt
     3061 gatctacgaa gtgcacgtgc acggatttac cgcgcggcac ccggatgtcc ccccgaacct
     3121 gcgaggcacc tatgccggcc tcgcgacgcc ggaggtgatc gagcatctgc aacacgtggg
     3181 cgtcacggcc gtcgaactct taccggtcca tcatttcgtc cgcgacaaac atctggtcga
     3241 ccgaggtctc accaattact ggggctataa ctcgatcgga ttcttcgcgc cggacatccg
     3301 ctacgccgcc tcaccggagc gcgcccacca cgtctgggag ttcaagacga tggtcaaggc
     3361 gctccacaac gccggtatcg aagtcatcct cgacgtggtg tacaacccca caggagaagg
     3421 caaccatctg ggcccgacgc tgtcgttccg tgggatcgac aacgcctcgt actaccggct
     3481 ggtccccgac catccgcgct tctatcaaga ctacaccggc tgcggcaaca cgctgaacgt
     3541 ccgccatccc cgggtcctcc agctcatcat ggacagcctg cgctactggg tgctcgaaat
     3601 gcatgtggac ggcttccgat tcgatctcgc ctccacgctg gcgagggagc tgcacgacgt
     3661 ggaccgtctc agcgcatttt tcgacatcat tcatcaagat ccggtgctct cgcaggtgaa
     3721 actgatcgcc gaaccctggg acttaggcga aggcggctac caggtcggta actttccggc
     3781 cggctgggcc gagtggaacg gcaggtatcg cgacgcgatc cggcgatatt ggaaaggcga
     3841 cgggggacaa gtggcggagc tcgcctatcg gctctccggg agcagcgatc tctacgaagg
     3901 aggcggccgc cggcctcacg ccagcattaa ttttgtaacg gcgcacgacg ggttcaccct
     3961 ccacgatctc gtctcctata accaaaagca caatgaagcc aacggggaag acaaccgtga
     4021 cgggaccgac gacaatttga gctggaattg cggcgtggag ggtccgacga acaaaccgtc
     4081 gatcgccgcg cttcgcgaac gacaaaagcg caacatgctg gcgacgctgc tcttgtcaca
     4141 aggtgtcccg atgctctgca gtggcgacga aatcgggcgg acacaaggtg gcaataataa
     4201 cgcctactgc caggacaatg agatcagctg ggtggactgg acgctgagca aaccgcagca
     4261 ggcgctcttg accttcacca ggggcctgat cgcgctgagg cagaaacatc cggtgttccg
     4321 gcgccgccgg ttctttcaag gccgccggat ccgtgggatg gaggtcaaag atctttactg
     4381 gctgcgcccc gacggaaaag agatgacgga tgaagactgg acccaaggct atgtccgttg
     4441 cctgggcgtc cgattggcgg gtgacgccat ggaagagaaa gacgcgaagg gaagaccact
     4501 cctggacgaa acctttctcg tgttgctgaa tgcccaccat gaacccaggc cgttcacgct
     4561 gcccgcgcac aagcgcggcg tgcgatggca accggtgttc gacaccgcca gctatgacac
     4621 gaacggaaag ccggtcacgc ttttcagagg gggagatcaa tatgatctcg aagcccgctc
     4681 cctcgccgtg ctccggttac gttcgaaaca gccttccgcc tcataaaccc tgggcgttgt
     4741 tttccacccg cccgacccac cgattgcttt gcaatcgatt tcccgggaca cgcccttttc
     4801 ccaggcgagc accgcctaac aatggatccc tccaagctcg ctcgtttcct tccgtacgga
     4861 ggtggatggt cgactgagcg acttgcgatg ggtccagggc cttgtcgcta cacggtcgcc
     4921 gcaggcgatg gcgagacgca ctgttcgaag ttccgttcgg acgtagtcga gaacggaaca
     4981 agtttgcgga gccaagtagt gacatgagcc tagggcctca cccatcgctg gagcaaacac
     5041 gaccatccat atccgtaagc tcgctctagg gggcactcca gttccacatt cctacgcaga
     5101 cgcagagaat cgaggcgtcg acgcggcttt gtgagctcgc aaggaggtag cagatggacg
     5161 cgaccagac
//
LOCUS       DASSCQ010000169         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_13015, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000169 DASSCQ010000000
VERSION     DASSCQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13015"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFH05_11460"
     CDS             complement(<1..437)
                     /locus_tag="VFH05_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678881.1"
                     /translation="MSRTAEPASVTVSTPSVLLNPSHTQPRPAAVGSADRIRVMKFVG
                     IFAIGGTERQVVNIGKGLDKSRFDLRFATLNKVGELLAECDAQSWAIDEYKIRRLYGL
                     ATMRKQIEFGKSLRRARTQILHAYGIYPNVFAIPAARLAGVPVT"
     gene            complement(407..1741)
                     /locus_tag="VFH05_11465"
     CDS             complement(407..1741)
                     /locus_tag="VFH05_11465"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET6678882.1"
                     /translation="MNHAQIQWWRPESATREWRSEDGQPAGYWEPPNTAVPFWATMVF
                     IAILLFSPQAYFPALESIRPAMIPAVIGIGAYVLDRVGSRTPFFIPRREVWVAGALLA
                     WATVTIPLSIWPGGSANFLVGYFLKTLAMSWLLSHSAATLGRLRQTTWCLSIMAVGLG
                     LFAFNNYLTGVFVERERVVGNEGALTKNPNDLALMVNIIVPLTMALFLSNRRPVIRLA
                     LFGILVVEGLTVVSTFSRAGFLTLGAILLTYAWKLRRRAERVWVNAILIFALLLIPLL
                     PASYFDRMSTIIHTEQDATGSAGERWSDMMIAVRSALSSPVIGAGIGMNVLAMNEARG
                     GEWRPVHNVFLELMMDLGVPGLLLFAWLLWTSVTGSADVHRQANQTVEMQELAYLAEG
                     IHISLLAFALAAMFHPVSYHPYFYYLAALALAAQAVLQSMRNTCLEQLNRHP"
     gene            complement(1754..>2084)
                     /locus_tag="VFH05_11470"
     CDS             complement(1754..>2084)
                     /locus_tag="VFH05_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HET6678883.1"
                     /translation="VMSRARLVLTDSGGIQEETTVLGIPCLTLRSNTERPVTLTHGTN
                     RLIGTNPEAIVSMALQALNESARSSDRPPLWDGLASERIIEKLDGRRLRNSADPMAVR
                     EAASTFS"
BASE COUNT          472 a          607 c          615 g          390 t
ORIGIN      
        1 gtgacgggta cgcctgcgag tcgcgcggct ggaatggcaa acacattcgg gtagatgccg
       61 tacgcatgca ggatctgtgt tcttgcccga cgcaaggact ttccaaactc gatctgcttc
      121 ctcatcgttg ccaagccata gagtcgacgg attttgtact cgtcaattgc ccaggattgc
      181 gcatcgcatt cagcgagaag ctcaccgacc ttattcaagg tggcgaaacg taagtcgaag
      241 cgtgacttgt ccaaaccctt tccgatgttc acgacctgac gttcggtacc gccaatagca
      301 aagatgccga cgaacttcat cacccgtatg cgatctgcgg atccgacagc cgccggccga
      361 ggctgagtgt ggctcggatt gagcaggacg ctcggcgtag agacagtcac ggatgccggt
      421 tcagctgttc gagacatgta ttcctcatag actgcaagac ggcctgtgca gcgagagcta
      481 aggccgccag atagtaaaag tatggatggt aactcacggg atggaacatg gccgccaacg
      541 caaaggccag caggctgata tggattccct cggccagata ggcgagctct tgcatttcga
      601 ctgtctggtt ggcctgtcgg tgcacatccg ccgagccggt cacgcttgtc catagtaacc
      661 acgcaaacaa caggaggccc ggcacgccaa ggtccatcat gagttcgagg aaaacattat
      721 gcacaggtcg ccattctcct ccccgcgctt cgttcatcgc gagtacgttc atgccgattc
      781 ccgcaccgat gaccggcgac gacaaagcgg aacggacggc gatcatcatg tccgaccacc
      841 gctcgcctgc cgagcctgtc gcatcctgtt cggtgtggat gatggtgctc atgcggtcga
      901 agtagctggc gggcagcagc ggaatgagaa gcagcgcgaa aatcaggatg gcattgaccc
      961 agacccgctc ggcccggcgt cgaagcttcc acgcataggt gagaaggata gcccccagcg
     1021 tgaggaagcc tgctcgggag aaggttgaga cgaccgtcag cccttcgacg acgagtatgc
     1081 cgaacaatgc cagacgaatc accggccggc ggttgctcag aaacaacgcc atcgtgagcg
     1141 gaacgatgat gttcaccatc aacgcgaggt cgttgggatt cttggtcaag gccccctcgt
     1201 tgccgaccac gcgctcacgc tcgacaaaga cccccgtgag gtagttgttg aacgcgaaga
     1261 gtcccagccc gaccgccatg atgctcaagc accaggtggt ttgtctcagt cggcccagag
     1321 tggctgccga atgactcagc aaccaggaca tggcgagggt tttcaaaaag tatccgacca
     1381 aaaaattcgc actgccgcct ggccaaatag acaaaggaat ggtgacggtc gcccatgcga
     1441 gcaaggcccc ggctacccac acttctcgtc gagggatgaa gaacggcgta cgagaaccga
     1501 cccgatccag cacatacgca ccgataccga tgacggcggg tatcatcgcc ggccggatcg
     1561 actccaaagc ggggaaatag gcttgcggag aaaaaagaag gatggcgatg aagaccatcg
     1621 tggcccaaaa gggaacggcc gtgttcgggg gctcccaata accggccggc tggccatcct
     1681 cggaccgcca ctcgcgggta gccgattccg gcctccacca ttgaatctga gcgtggttca
     1741 tggggtcgta ggttcaggaa aaggtggacg ctgcctcgcg gacggccatg ggatcggcgc
     1801 tgtttctcag ccgccgtcca tccagctttt cgataatgcg ttcggatgcc aggccatccc
     1861 agagaggagg ccgatcgctt gacctggcgg attcattcag cgcctgcagg gccatcgaga
     1921 cgatcgcctc aggatttgtg ccgatcagcc ggttggtgcc gtgggtcaag gtcactggtc
     1981 gctcggtatt gctgcgcaag gtcagacacg gaatgcccaa cactgtcgtt tcctcttgaa
     2041 taccccctga atccgttagg acgagtcgag cccggctcat cacg
//
LOCUS       DASSCQ010000170         6391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1064406, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000170 DASSCQ010000000
VERSION     DASSCQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064406"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VFH05_11475"
     CDS             <1..1096
                     /locus_tag="VFH05_11475"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HET6678884.1"
                     /translation="GTIVKFPGHTIVYMEGVDKELFAQKPKQEEEAEETAERQLPELT
                     EGERLRLVEQEEQTIPGVTSKQHFTQPPPRYNEALLIKELEDKGIGRPSTYAAIISTI
                     QERKYVEKVDGRFLPTETGRTVNDFLLKGFPDVVNVDFTSQLEAELDEVEEGNKPWVD
                     AVRDFYDPFTADLEKAKNIPGPKDTVEPPTNIPCEKCGRMMEIKWGRNGKFLACPGYK
                     EDPPCKNTQNFEKLPDGTIKIIPKQELTTDQVCEKCGSPMLVKTGRFGKFIACSAYPE
                     CKTTKPLALGVKCPQPGCGGDLVQKRTRKGRSFFACSKYPACEYALWDRPIPKACPTC
                     SAPFLIEKVSKQAGRSVQCRNEDCGYREAG"
     gene            1338..1814
                     /gene="bfr"
                     /locus_tag="VFH05_11480"
     CDS             1338..1814
                     /gene="bfr"
                     /locus_tag="VFH05_11480"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET6678885.1"
                     /translation="MKAKEGVVNLLNKILTADLTAINQYFVHGKMCENWGYERLYHKV
                     RERSIDEMKDADELIGHILYLEGLPNVQRMNTVHVGETVPEQLKLDLKAEQEMLALLN
                     DGVVHCAKVMDFTTRHMLEDMAKDVDSHIDWIETQMETIKQIGLENYLAEQIKKES"
     gene            complement(1913..3637)
                     /locus_tag="VFH05_11485"
     CDS             complement(1913..3637)
                     /locus_tag="VFH05_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678886.1"
                     /translation="MKRLSLRFLLLTSPIGLFILTLVGACSGSGSSTPPTAQVTGTPG
                     AEQAVGERLFMETRFAQAFQAFLDAGGDVNDPNAGDPVVDSVETLGVPITAEAPFKGM
                     SMNCRSCHFVDDLLAAPGGSMRTYTDFAKRSPIPARADGKTHAPRNSPPLVNATLGRP
                     GGMLLHFDAEFNSIEELVAGTFTGRNFGWLPGERAQAIAHIARVVRGDDGSFDPSGQF
                     EGLSYRILFTGNNPSIPDESRLPPEFRAFVGSASDQEIFDAVVKVVSAYVNGLLFSQT
                     EDTGALIRSPFDVFLETNGLPQQPDLSESPIDYSRRLRTLVNGLASPQFVTSNPNRSD
                     GQFQFHSQPFEFGPNEFAGLKMFLAEPAAPVASPAELTAGRIGNCIACHQAPNFTDFK
                     LHNTGTTQKEFDSLPSHGPGAFSVLSIPTLVTRTANDLPASEAHPTASERFRSIPSDG
                     TTLTDLGVWNVFGNPDMPATQMKIRTILCDDQQPCPLSETVLLDRAIARFKTPGLRDL
                     DHSAPYMHNGQFTTLDEIITFYRDTSTLARAGTLRNGAPELKGIALTAADAVPLVGFL
                     KALNEDYQ"
     gene            complement(3657..4328)
                     /locus_tag="VFH05_11490"
     CDS             complement(3657..4328)
                     /locus_tag="VFH05_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6678887.1"
                     /translation="MIHPTALEIFRILAECDSIPPSASQHNFEVTFARRAACLLDEAL
                     PWQRGHGRRTAHLAQRLGLAAGFCREAIHHLTLAALLHDIGLLALPSSLASPTGYLDL
                     HAYAARQSHPRLGAQWLEPYRFLRKASVIIAHHHERWDGSGYPYGMRGTTIPIEARVL
                     AIADAFDAIDVIGVDDPKMRDEASYRIIRSASGTQFDAHLVKLCPLACSHQIGPSEHP
                     HQSVA"
     gene            complement(4425..5951)
                     /locus_tag="VFH05_11495"
     CDS             complement(4425..5951)
                     /locus_tag="VFH05_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6678888.1"
                     /translation="MRMRFLIVAMSLSAMLPLSAWAEDSVMEQRMKKLEEDVRQYKNE
                     LRTYQERERRESPAERRMEPIEGPPGSQYPMATGPMTDVRVERKGEEKVPLGFGSTGS
                     GRLVYAKPFVAAPKAIVGGYMDIQYRSHRNGVLETGDYNSIRGGQGTTNGFDQQRFVP
                     FIYADITEHVKFASELEIEHGIRGAGEIEISLEFAHLDYLVSEPFNIRAGILLIPIGK
                     FNLLHDSPLNDLTDRPLVSQFVIPSTMSETGAGVYGTFYPGRTSKLDYELYFSTGPCG
                     YNSDGSPRINEETGTKDSRQRKCEQSADGLDINNGKAVSGRVAFSPMLGVEVAGSGYF
                     GNSSPSSYNPLSITAIDWTLQRGPFELIGEAAWAYARGNSRAIPGNGFGSSPGSLLTG
                     VNTFNPLAAPPQRMEGYYIQANYHFMPAFLANWSPKRFGEGSTFTAVIRYDRVNLNMD
                     NKGENQGDLEQVSFGLNYRPVEDAVFKISYQYLPKSFNPNSGQRIHDSAFVISAATYF
                     "
     gene            complement(6143..>6391)
                     /locus_tag="VFH05_11500"
     CDS             complement(6143..>6391)
                     /locus_tag="VFH05_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678889.1"
                     /translation="TNATSGLKALVTWGGFGGVILDLISPWLITYVSPIFVLTMLTGD
                     MLMMLSFLVMMAIPMYEMWILQQPLMAGKRPDDPMSSR"
BASE COUNT         1516 a         1700 c         1790 g         1385 t
ORIGIN      
        1 cgggaccatc gtgaagtttc ccggccatac gattgtctac atggagggag tcgacaaaga
       61 actcttcgca cagaagccca agcaggagga agaggctgag gagacagcgg agcgccaact
      121 tcctgagttg acggagggcg aacgtctgcg tctggtcgaa caggaggagc agaccattcc
      181 cggggtgacg tcaaagcagc acttcactca gccgcctccg cgctacaacg aggcgctgtt
      241 gatcaaggaa ctggaagaca aaggcattgg aaggccctcc acctatgcgg ccatcatttc
      301 gaccatccag gagcgcaaat acgtcgaaaa agtcgacggg cggtttcttc caactgaaac
      361 cggccgtacc gtcaacgact tcctgctgaa agggtttccc gatgtcgtga atgtcgactt
      421 tacctcacag cttgaagcgg aattggatga agttgaagaa ggcaacaaac catgggtcga
      481 cgccgttcgc gatttttacg acccgttcac ggcggacctc gagaaggcca agaacattcc
      541 cgggcccaag gataccgtcg agcctccgac gaacattccc tgcgagaaat gcggccggat
      601 gatggagatc aagtgggggc ggaacggcaa gttcctggcc tgccccggct acaaggagga
      661 tccgccctgt aagaacaccc aaaacttcga gaaactgcct gacggcacga ttaagatcat
      721 tccgaagcag gaactcacca ccgaccaggt gtgcgagaaa tgcggcagcc cgatgctggt
      781 gaagaccgga cgcttcggca agttcatcgc ttgctccgcg taccccgagt gcaaaacgac
      841 taaaccgctc gccttgggcg tgaaatgtcc acagccgggc tgcggtggtg atctggttca
      901 gaagcgcacg cggaaaggcc ggtcattttt tgcgtgcagc aagtatccgg cctgtgaata
      961 tgccttgtgg gatcgtccca tcccgaaggc ctgccccacc tgtagtgccc catttctgat
     1021 tgagaaggtg agcaagcaag ccggccgaag cgtgcagtgt cggaacgaag actgcggcta
     1081 ccgcgaagcg ggataagcgc gtctccgcac gccctgcttt ttcgtctcat tcctcttgac
     1141 ccccttgatc acatagtcaa tcttcgttcg acgttcctct tcgctcgtcc gagattgaga
     1201 atacatgtcg tcgacactga gagatgaaat caaaatgcgt cgtcctgtga gttgacgagc
     1261 gctgtcagtc atgatgataa tgaatcacgt acctctgcag aaacggcatt ttactcgttg
     1321 gtgaagggag gaccgctatg aaggcaaagg aaggagtggt gaatctcctc aacaagattc
     1381 ttacggccga tctgacggcc atcaaccaat attttgtgca cggaaagatg tgcgaaaatt
     1441 ggggctacga gcgcctctat cacaaggtgc gcgaacgcag catcgacgaa atgaaggacg
     1501 cggacgaatt gatcggacac attctgtatc tggaaggcct tccgaacgtc cagcgaatga
     1561 ataccgttca cgtcggagag accgtgcccg aacagctcaa acttgatctg aaggccgaac
     1621 aggagatgct ggcactgttg aacgatggcg tcgtccactg cgcgaaagtc atggatttca
     1681 ccacgcgtca catgctggaa gacatggcca aggatgtgga ttcgcacatt gattggattg
     1741 aaactcagat ggaaacgatc aagcagatcg gattggaaaa ctatctcgcc gagcagatca
     1801 agaaagaatc gtaaggcaaa gagggcggga ggccccagga ggaagcaagg cctcccgcca
     1861 gtagagcagc gcaagtcgtc cgtctcccgc tccgatttcc tgcactgtct ccttattgat
     1921 agtcctcatt cagcgcttta agaaatccaa ccagcggcac cgcgtcggcc gcggtgagcg
     1981 caataccttt gagctcggga gccccgttcc tcagcgttcc cgcgcgtgcc agcgtcgacg
     2041 tatcccgata aaaggtaatg atctcgtcaa gcgtcgtgaa ttggccattg tgcatgtagg
     2101 gggcggaatg atcgagatca cgaagtcccg gagtcttgaa tcgggcgatg gcacggtcca
     2161 agagaacggt ctcggacaag gggcaaggct gttggtcgtc gcagaggatg gtgcggatct
     2221 tcatttgggt agccggcatg tccgggttgc cgaagacatt ccagactccc aaatccgtca
     2281 gggttgtgcc atcagacgga atggaccgga accgttcact ggccgttgga tgcgcctcgc
     2341 ttgcgggcag atcattggcc gttctcgtca ccagggtggg gatggacaaa acagagaatg
     2401 cccctggacc atgacttggc aaactgtcga actccttctg tgtcgttcca gtattgtgta
     2461 acttgaaatc cgtaaagttc ggcgcttgat gacaggcaat acaattgccg attcttcccg
     2521 cggtcaattc cgccggcgaa gcgaccggtg ccgccggttc cgctagaaac attttcagcc
     2581 ccgcgaattc attcgggcca aattcaaatg gttgagagtg aaactggaat tgaccgtctg
     2641 atcggttggg attcgaagtc acaaactgcg ggctcgctaa accattgacc agcgttcgca
     2701 ggcgcctgct gtagtcgata ggcgactcgc taaggtccgg ctgctggggg agcccgtttg
     2761 tctcaaggaa cacgtcgaag ggtgaacgaa ttagagcgcc ggtatcctcc gtctgcgaga
     2821 agagcaggcc gttcacatat gcggacacga ccttgacgac cgcatcgaaa atttcctggt
     2881 ccgaagccga tccgacgaat gcacggaatt ctggcggtaa ccgcgattca tcggggatcg
     2941 acggattgtt gccggtaaaa agaatacgat acgaaagccc ttcgaactga ccggacggat
     3001 cgaatgaccc gtcgtcgccc ctcaccactc gggcgatgtg ggcgatcgcc tgtgctcgct
     3061 cgccggggag ccaaccgaaa ttgcgtccag taaaggttcc tgcgacgagt tcctcgatgg
     3121 agttgaattc tgcgtcaaaa tgcaaaagca tgccgcctgg gcggccgaga gtcgcgttga
     3181 ccagcggagg cgaattgcgc ggcgcatggg ttttgccgtc ggcgcgggca ggtattggac
     3241 tccgcttggc aaagtcagta taggtccgca tgctgcctcc tggagccgcc aacaagtcgt
     3301 caacaaagtg gcaggagcgg cagttcatcg acatcccttt gaacggtgct tcagcagtaa
     3361 tcggaactcc taaggtttca actgaatcga caacgggatc accggcgttg ggatcattga
     3421 cgtcaccgcc ggcgtccaaa aatgcttgaa acgcttgcgc aaagcgcgtt tccataaaca
     3481 accgctctcc gacagcctgc tctgcccccg gtgttccagt tacttgagcc gtaggcggtg
     3541 ttgaagaccc gctacctgag caggctccaa caagagtgag gataaaaagt cctatcggag
     3601 aagtgagcag taaaaatcgc aagctcaagc gtttcatgat gactcccttt agacggtcag
     3661 gctacagact gatgtgggtg ttcagatggc ccgatttggt ggctgcaggc gagcgggcat
     3721 aatttgacca ggtgggcatc gaattgcgtg cccgaagcgg atcgaatgat gcgatagctc
     3781 gcctcatcgc gcatcttggg atcatctacg ccgatcacat caatggcgtc gaatgcatcc
     3841 gcaatggcaa ggactctggc ctcaatcgga atggtggttc ctcgcattcc ataaggatat
     3901 ccggatccat cccatcgttc gtgatgatgt gcaatgatga ccgaggcctt ccggagaaag
     3961 cggtagggct ctagccattg agcgcccaat cgcggatgcg attggcgggc agcataggcg
     4021 tgaagatcca ggtatccggt aggcgacgcg aggctgctgg gcaaggccaa gagcccgatg
     4081 tcatggagca gtgcggcgag cgttagatgg tggatggctt ccctgcagaa tccagccgct
     4141 aagccgagtc tctgtgcaag gtgtgcagtt cgccgaccat gtcctcgttg ccagggaaga
     4201 gcttcatcca agaggcacgc cgcccgtctg gcgaacgtca cttcaaagtt gtgctgtgag
     4261 gcggatgggg gaatgctgtc acactctgct aatatcctga aaatttccag agccgtagga
     4321 tgtatcatga ctgcctcgcg tcccgcacaa ataagtggct gtaagtgatc ccgcgctctc
     4381 agggattcgg cgcctccatc ggggaggatt tacaagccgt atatctagaa atacgtcgcg
     4441 gcagagatca caaatgcgct gtcatgaata cgctgacctg agtttggatt aaaagacttc
     4501 ggcagatact ggtagctgat cttaaacacg gcatcttcga ccggtcgata gtttaagccg
     4561 aacgacactt gctcgaggtc gccttggttt tcccctttat tatccatgtt gagatttacc
     4621 cggtcgtacc gaataacggc cgtgaaggta gatccctctc cgaatcgttt cggtgaccag
     4681 ttggcaagga acgcaggcat gaaatgatag tttgcttgga tgtagtatcc ctccatccgc
     4741 tgtggtggag cggccaacgg attaaacgta ttgacacccg tgagtaagga gccaggactg
     4801 gaaccgaaac catttccagg aatcgcccgg gagttacccc gagcataggc ccaggctgct
     4861 tcaccgatca gctcaaacgg ccctctttga agtgtccagt cgatcgcggt gatacttaag
     4921 gggttatagc tcgatggtga tgaattgcca aaataccccg agccggcaac ttccacgccg
     4981 agcatcgggc tgaaggccac acgacctgag acagcctttc cgttgttaat gtcaagaccg
     5041 tcagcgctct gttcacattt acgttggcga gaatcttttg tgccggtttc ttcattgatt
     5101 cgtggagagc catcactgtt gtacccacag ggcccggtag aaaaataaag ttcatagtcc
     5161 aatttacttg ttcggccagg atagaaggtt ccgtaaactc ctgcgccggt ttctgacatg
     5221 gttgatggga tgacgaactg gctgacaagt ggccgatcgg ttaagtcgtt caagggcgag
     5281 tcgtgcagca ggttaaactt gccgattgga atgagcaaaa ttcctgcacg aatattgaag
     5341 ggttcactca ccaggtagtc gagatgagcg aactccaaac tgatttcgat ttcgccggcc
     5401 cctctgatcc catgttcgat ctcaagttct gaggcgaact ttacatgctc ggtgatatcg
     5461 gcataaatga acggcacgaa tcgctgttgg tcgaatccat tggtcgtacc ctgtccaccc
     5521 cgtatactgt tatagtctcc tgtttcgagg acaccattgc ggtgacttcg atattgaatg
     5581 tccatatagc cgcccacaat agctttcggc gccgcgacaa acggcttagc atagaccaaa
     5641 cggcctgatc cggttgagcc aaagcccaaa ggaacctttt cttctccctt ccgctcgact
     5701 cggacatcgg tcatcggccc ggtagccatg gggtactgac ttccaggggg accttcaatt
     5761 ggctccatcc tccgttccgc ggggctttca cggcgttcgc gttcctgata cgttcgtaat
     5821 tcatttttgt actggcgcac gtcttcttcc agtttcttca ttcgttgttc cattacagaa
     5881 tcttctgccc aggctgacaa tggcaacatg gcagacaggc tcatcgccac gatcaagaat
     5941 cgcatcctca tataaccctc caaaaacaaa atggtgtatg agtgctcgac tcggggcgct
     6001 gcggcctctg gtctgtcggt caacgctaca tgtttctcgc tgatcgttga ggtgtgaaaa
     6061 gcaaaataaa aagccagaaa tcacgaaccg tgatttctgg ctctgggcga ataacctctg
     6121 gccgaactac attgtgcgaa tgttagcgcg acgacatggg gtcgtccggt cgtttccccg
     6181 ccattaacgg ttgctgcagg atccacattt catacatggg aattgccatc atcacgagaa
     6241 aactcagcat catcagcatg tcgccggtca acattgtcag cacgaagatg ggcgagacgt
     6301 acgtgatgag ccagggcgaa atcaggtcga ggatcacgcc gccgaagccg ccccacgtca
     6361 ccaaggcctt gagcccgcta gtcgcattgg t
//
LOCUS       DASSCQ010000171        20624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_892515, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000171 DASSCQ010000000
VERSION     DASSCQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 20624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20624
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892515"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFH05_11505"
     CDS             <1..1002
                     /locus_tag="VFH05_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6678890.1"
                     /translation="PLKQLLTIGRFAGLSPAERWKLLSWLEQLWEGSVQLAPDLEHRT
                     AQQWLESLDVRRTSLQTIWNPLARWLTGSDIQSLSADAFVTALKPFFLSGAANSRIWV
                     AHPSRELLVEPIKDHLTKDGATLAFRTQAVRLEFDHERVTGVRVGDGSVLEADWYLAA
                     VPPQELTPLLPERWLSRYAYFQHIADLEMSSSTFLHVRTATILTKPRHILLSSGPLHW
                     MMCSSRAPDRRLLAVIKIQPDHPAPDLERHVSTLLGSLHLLEPGRHPDGLGQHEIQHA
                     VLPLKPGTKLLRPIPRSPIPNLLVAGAWTDTGWPANLESAVVSGERCADIITGRLSA"
     gene            1082..1723
                     /locus_tag="VFH05_11510"
     CDS             1082..1723
                     /locus_tag="VFH05_11510"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6678891.1"
                     /translation="MTLQELAKSLHNCQQCKLAKLGRTQVVFGVGNPRASIMFVGEAP
                     GFNEDQKGEPFVGAAGKLLNDLLASANLSRDQIYIANVIKCRPPNNRDPEPDEVETCK
                     PFLMQQIQMIRPKLVCTLGNWATQTLLERKVGITKVKAQAFYLKDFVIFPLLHPAAAL
                     HQGNLLDTLKEDFRKLKEFLDRHSQPPPEEAPAPAVSALNIEPPNPAQMDLFG"
     gene            complement(2247..2714)
                     /locus_tag="VFH05_11515"
     CDS             complement(2247..2714)
                     /locus_tag="VFH05_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678892.1"
                     /translation="MPLDAELGKKMLQLVTSRYDDRHWRKKIEKTLSLPQSGVGDPLQ
                     QQIFMYLKVGLKAYKSRRADPDSWIIGGYATKEVIERAKHQPKQVGADVTKDDVAFLG
                     NDPGTEVDEAWWDDMLVQWFDVPEEDKQVEGESEEQVTEPTSATSPERKPTKA"
     gene            complement(2771..3328)
                     /locus_tag="VFH05_11520"
     CDS             complement(2771..3328)
                     /locus_tag="VFH05_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET6678893.1"
                     /translation="MRRACQMMLLAALLFPTAGMAADFRLSSPTVKDKGTIGHEHVFN
                     GFGCTGNNTSPELRWERAPKGTKSFAVTVYDPDAPTGSGWWHWVIFNIPPDTTSLPAG
                     AGKPESGAAPQGSIQSMTDFGQPGYGGPCPPPGDKPHRYIFTVYALKADQLPLQKEAS
                     GAMVGYYLNQNKLAKASFTSKYSRK"
     gene            complement(3415..3945)
                     /locus_tag="VFH05_11525"
     CDS             complement(3415..3945)
                     /locus_tag="VFH05_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET6678894.1"
                     /translation="MLVAPLALILPAMAVSARGEGNMHITSSAFTNQGAIPVLYTCEG
                     QDVSPPMAWSDVPPGTKSLVLIVDDPDAPDPAAPKMTWVHWVLYNLPPTANGLPERVK
                     TLPEGTREGVNDWKRTGYGGPCPPIGRHRYFHKLYALDTLLPDLKQPAKSKLEEAMKG
                     HILSEAQLIGTYQKGG"
     gene            complement(4006..5391)
                     /locus_tag="VFH05_11530"
     CDS             complement(4006..5391)
                     /locus_tag="VFH05_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET6678895.1"
                     /translation="MHQENPDRAIQPANGHLLQLAADVLAMAKKDGATEADVVVADGE
                     NLSVQVRLGAVDRLTKAREKRLGLRVFVGKRSATTSTSDFSSESLRRLVSETCTLARA
                     VVEDSMSGLPEPEQLAQEQPDLDIYDDTKVDAERQIELARRAESAAMATDERIANSEG
                     ADFDSSWGRVVLGNSHGFVGEYRSSSYSLAVSPVAVDPASGAMQRDAWYDVQRKFIKL
                     ETPEAIGIEAAKRTVRRLGARKIATKRVPVIFDRETAGSLLGNLCSAASGYGLYKGAS
                     YLAGQLGQQVAPDWITVVDDGRMIGGLGSRPFDGEGLPTRRNTIIDKGMLASYLLDTY
                     SGKKLGLPSTGNASRSVGESPSVGPTNFYLVPGTTPAEDIIRSVKDGLYVTELIGFGI
                     NMVTGDYSRGAGGFWIENGQLAYPVEEITIAGNLKRMFMDIESVGSDLVFRGRIASPT
                     LKIAEMMVAGS"
     gene            complement(5408..6841)
                     /gene="tldD"
                     /locus_tag="VFH05_11535"
     CDS             complement(5408..6841)
                     /gene="tldD"
                     /locus_tag="VFH05_11535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HET6678896.1"
                     /translation="MAQLIPLDSFGVTERDVEGALGRVKVRNVDYADLYFESCVSESV
                     SMEESLVKRATKSISQGVGVRATAGEKTGFAYSDELTKRDLELAADTARYIAHSPGGT
                     EPVPVPVRSRPTRDLYPVSHSQLEVATAERVALLNRIDEEARRYDPRIKNVMASFNTE
                     YKIVMVATSEGTLIGDVQPLSRLQVTCIAEEHGNRQIGSFGGGGRIGFEFYRERDRYL
                     DYAKEAARIAILNLSSVDAPAGVMPVVLGGGWPGILLHEAIGHGLEADFNRKKTSAFS
                     NLIGKRVASEVCTIVDDGTLPGRRGSLNMDDEGTPTSRTTLIEKGILRGYITDRLNAH
                     LMGIPVTGNGRRESYQSIVLPRMTNTFMLAGQSDPDEIIRSVDKGLYAVSFGGGQVDI
                     TNGKFVFSASEAYLIEGGKITRPVKGATLIGSGPDILTKVSMVGHDLKLDEGIGTCGK
                     EGQSVPVGVGLPTIRIDQITVGGTQAA"
     gene            7061..7846
                     /locus_tag="VFH05_11540"
     CDS             7061..7846
                     /locus_tag="VFH05_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET6678897.1"
                     /translation="MDTQPPSRIDDLSETELTAKLDPDLLPKHVAIIMDGNGRWAEAR
                     SLPRIAGHREGIKSVRDMISLCLELGIQALTIYAFSQENWNRPTTEIDGLMGLLEHYL
                     STERNKLIEQGVRFRTIGRTEALPTSALQWVRAAERETARMEKLQLTVALSYGGRAEI
                     VDAVRGLVRDAQEGKLQPEDIDEPQIQQYLYTRSLPDPDLLIRTSGETRISNFLLWQL
                     AYTELYFTPTLWPDFRRRELLLALLEYQRRERRFGRVFSSLSS"
     gene            7867..8742
                     /locus_tag="VFH05_11545"
     CDS             7867..8742
                     /locus_tag="VFH05_11545"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET6678898.1"
                     /translation="MLECGTTNRDSAHTVHKAAGRFDARRIYTVLFVVPLLYTAIRFF
                     PPAVFTGLVAVAGCLALRELYRLCFQVVRQPLMVGIGLAGCVALIVSPHVPALAQPGL
                     LVSLVAILSIPLLIRTPLDQSVKDAALTLTGMMYIGLMLGYLVAVRLLPQGEWLLFFL
                     LLVTWAADTGAYYVGTLYGQRPLAPRISPKKTIEGLAGGLFAAVLIAFVARWGFVSDF
                     SHWDGLILAVLLTFAGLWGDLVESAIKRSVGVKDSGGLLPGHGGMLDRLDSLLFTAPT
                     FYYYVTSIRDIAPSP"
     gene            8952..10112
                     /locus_tag="VFH05_11550"
     CDS             8952..10112
                     /locus_tag="VFH05_11550"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET6678899.1"
                     /translation="MKSIVILGSTGSIGTNTLDIVERFPEEFRVVGLTAGNNDEMLAE
                     QIRRFHPLAVAMPTESGAARLKQRCGGQPVEILSGPEGLAAVASLPEAELVVSAIVGG
                     AGLVPTLAAIRSKKHIALANKEPMVMAGQLMQEEARRQGVRIFPVDSEHSAIFQSLEG
                     HRIKDVRRLILTASGGALWNVPKEQLQDVTPERALQHPNWKMGAKITIDSATLMNKGL
                     EVVEARWLFDIPESRIDVLIHRESIIHSLVEYEDRSMISQLGLPDMRTPISYAMRYPD
                     RLPLDLPSLDLTEIGTLTFCKPDHDRFPCLRLGYESLRIGGTMPAAMNAANEIAVDAF
                     LNGGIRFTDISDIIQSTMQTHVPQAVRSLEAALEADRWAREKADSLVHALAR"
     gene            10164..11561
                     /gene="rseP"
                     /locus_tag="VFH05_11555"
     CDS             10164..11561
                     /gene="rseP"
                     /locus_tag="VFH05_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET6678900.1"
                     /translation="MMASVLAWSPDSVWLLLQKTWWFLVVLGILVAFHELGHFLAARW
                     VGVKVLKFSLGFGPKLLGRQVGETEYLVSAIPLGGYVKLFGEEETEAITPEDRRRSFS
                     HQSLWGKVLIVSAGPGFNFILAYLIFAAWLATGYTLPVPSFKDIAPVLEAVAPDSPAA
                     RAGLMVGDQIKKIDGRDIATRVELLDAAAKGKGAPLTLEVLRDGRIQTLTVTPIPAPA
                     PTQRNDDSGYYLGIEEIPPVVNEVVPGKPAAAAGFREGDRVLSIDGRTIHTWTQMTGL
                     VKDSPGKTLQVDVLRDGHRVSLSVTPLAESATVNGQAVQVGKIGIAGPDRSLMRSANP
                     LMAVYNGLEATWKWTELTIVGLYKMIVGDISSKNIGGPLTIAKISGEAAAQGPANVIF
                     LIALLSINLGVLNLLPIPILDGGHLLFFLIEGILRKPLGERQRELAQQVGLVLLVGVM
                     IFAFWNDLERIFFSH"
     gene            11567..13270
                     /locus_tag="VFH05_11560"
     CDS             11567..13270
                     /locus_tag="VFH05_11560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET6678901.1"
                     /translation="MRTSQLLLPTLREDPGEAETISHRLMLRSGLIRKVAAGIYTYLP
                     LGLRIIRKVEHIVREEMNRAGAQELLMPIASPAELWRETGRWDFYGKELLRFKDRHER
                     DFCLGPTHEEVITDLFRREVRSYRQLPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDKDDVGARTSYQKMYDAYHRIFSRCGLNFRAVEADTGLIGGTSSHEFMVLADTGE
                     ETVVYSESGTYAANVERAEALPPIETSREELRPLETVSTPGRRTVEEVTAFLKVEASK
                     LVKTLLYSTGTETVAVLIRGDHDANEIKIKRLLGVAQLELAGPATVEKVTGAPAGFAG
                     PVGLKLRIVADHAVKSATNVVVGGNRTDTHLLNANVDRDFQVEQFADLRNARAGDPSP
                     RKDGVLKATKGIEVGHVFMLGTKYSQSMNASFLDPNGKECLAVMGCYGIGIGRTAAAA
                     VEQHHDDKGIIWPYPIAPFHVHLIPLGQSGGVAEATTSLYHALETAGLEVLWDDRDER
                     AGVKFNDADLIGAPFQVIVGDKGLTEGLLEVKTRKTGHKTKLAPAEIPARILQMASQP
                     S"
     gene            13477..15804
                     /locus_tag="VFH05_11565"
     CDS             13477..15804
                     /locus_tag="VFH05_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET6678902.1"
                     /translation="MPTQPPTQNLQELQQKVEFAEHVKRITNQIHAASNLDQILLDLH
                     KDILSLFDAEDLTLFAFDADKKEIFYKVPHIDTIEEVRIPITEQSLAGFCAKYLRPVN
                     IADAYNVAELQGIHPSLNHDTSYDKRTGFKTRQVLTYPIVADNKYLMGVLQLLNKKSG
                     LRFTRKDEESVAEIAKALGIAFFNLKKVSTKKASTKFDLLVTNGKITQNELDNAIAEA
                     RKSISSDLESILMEKYKVPKLEIGRSIAQFHKCPYIEYSERTLVDIELLRNLNVDYLK
                     KNHWMPLKRDRTAIEILTDDPGDLDRVADIKRTFPGLNIRFAVSLRRDISQFLNSATG
                     GSDGQPGRKLDENVSDILGELVTEAQAEAMEESGAGGGLDENDSAIVRLANQIIADAY
                     RQTASDIHIEPYGEKRETLVRFRVDGDCFEYMKIPQSYRRAIVSRLKIMASLDIAERR
                     KPQDGKIKFKLSETREIELRVATIPTAGYNEDVVMRILAASEPLPLDKMGFSDRNLKH
                     IKDIAEKPYGIILCVGPTGSGKTTTLHSVLGFINTPDIKIWTAEDPVEITQYGLRQVQ
                     VQPKIDFTFAKAMRAFLRADPDVIMVGEMRDKETAEIGIEASLTGHLVLSTLHTNSAV
                     ETVTRLLDMGCDSFSFADAMLGVLAQRLARRVCKDCKEQYTGTKEEYEEMRLGYGPEF
                     WDKLAIPMDNTFRLSRGKGCETCNRTGFKGRVALHELLLGTDKLKRLIQTRAKTEEMV
                     RAAIEDGMTTLMQDGIQKCLQGLTTFKEVKAVAIK"
     gene            complement(15929..16306)
                     /locus_tag="VFH05_11570"
     CDS             complement(15929..16306)
                     /locus_tag="VFH05_11570"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678903.1"
                     /translation="MAELERQPQPSVELRRYPRHRVSAPFPCAFSLLGLKRWAAVDRN
                     GLGVVLDVSLGGAKVMSAAAMRPGDHLAVSFRMPDQASALQLDATVKWVNDQLFGLEF
                     LSVPLSVESRLKKFLSRSAYTCS"
     gene            complement(16313..16708)
                     /locus_tag="VFH05_11575"
     CDS             complement(16313..16708)
                     /locus_tag="VFH05_11575"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678904.1"
                     /translation="MQGHPVGLDGRRPSALNRFRRHPRVRINTPFACALSPVQSRSWL
                     RKPVINVGLVYDLSVRGARVSTEAPFKPGDQVALSLRLPKQIRSAEIAVATVRWTKDQ
                     FYGLAFRRLSLDAYDRLKRFVAIASRAVA"
     gene            complement(16863..17813)
                     /locus_tag="VFH05_11580"
     CDS             complement(16863..17813)
                     /locus_tag="VFH05_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678905.1"
                     /translation="MSYRYSSVDIIVGVGVCAIVFGALLMFVATTGTFLVGNPPTAMA
                     EGSHVTTESAWLQPALGQAIVERALLQIQTDRMTASATSEWRQALQSHRSLHTVIDSP
                     FAMVISRAKDIPREHEARVQTVMGRSIVNFTQRGIRSGVFSADLYLSDYNQRMIGATE
                     QRGKRMHEGFASTWQSLLGKSIVDASQEHLQRVEVVQEQLGSAILHVAQARMLLDEAW
                     SSNQYQLGSLMAALDRTGGMSEAVTRPVSAVAGQVEVARSSAGMPGIPDIPMGYLMAA
                     ALALFSIFFGGLMLSAATREAKALTEARRNTGRWVYRMAS"
     gene            complement(18121..18765)
                     /locus_tag="VFH05_11585"
     CDS             complement(18121..18765)
                     /locus_tag="VFH05_11585"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6678906.1"
                     /translation="MKPRTMSVCIAMLAGCLAAYTVKAAEPSFELTARYILEVVKAFR
                     TAYVLKVVEHARDGGVKPNENWQSDAHFIPLPAQFVKAAADQLDNFEIGLIGLTPVNQ
                     ANYPRTQAESDALIQLMKNRERSVTTFVDGDQFKAISADLALVQSCVDCHNHHPKASR
                     RDFQRWDVMGGLVVRLKRDARPEGSPIGPEPPGRSSGPIERLTPPLTAPPPWVR"
     gene            18927..20261
                     /locus_tag="VFH05_11590"
     CDS             18927..20261
                     /locus_tag="VFH05_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678907.1"
                     /translation="MWIRAVLCALLCLLTACGGSDSSSPSTSEAPPPGQQPPPGQQPP
                     PTSGPAGPWPLADLTVYSAAQSLTGTIIDASPDDAQNIWAVTPDALYVLRPGEATFRR
                     FTAADGLHVQTYTDPANGQQTVSNITAMAGGISNEVFVGYRGYEAPIPPPAPPPCCVA
                     NADFSDPRWHLGQADKVTLDPGGGIQVRRYEFLCDITVNCWEERSARRMIFGHNGTAA
                     GHLFIGFDHGVAHVFNDVFGDHTHIVTFWHYADGRNVMRMGEHYGLALYPNGEMIDGT
                     GYGIGSHEWNADPRAWVNGRFVWGFTTYGPAEPYNNGEHDLIVPEGYREDNRGAAVTP
                     DGTAWWVSLHTGLSSYNHTIAQGNTSLIRHYTDVAGLPAAGLMDIAADPDGSLWIVDN
                     SGRLLRFTPSTQAVQVWPGVSSVRRIVMDTTVTPRALYVSMGSQGLAVIRAR"
BASE COUNT         4433 a         6123 c         5744 g         4324 t
ORIGIN      
        1 ccgctgaagc aactcctcac gatcggacgt tttgcgggac tatcgccggc cgagcgttgg
       61 aaactcctgt cctggttgga acaactttgg gaaggctcgg ttcagctcgc cccagacctg
      121 gaacatcgca cggcacaaca gtggctcgaa tcgctggatg tccgccgaac ctctttgcag
      181 actatttgga atccgctcgc acgctggttg acgggcagtg acattcaaag cctgtccgca
      241 gatgccttcg tgaccgcact gaaaccgttc tttctttccg gcgcggccaa cagccgaatt
      301 tgggtggcac atccatcccg cgaactgctc gtggaaccga tcaaagacca cctgaccaaa
      361 gacggcgcga cgttggcttt ccgcacacag gctgtacgac ttgagtttga ccacgagcgg
      421 gtgaccggcg tacgggtagg agacggaagc gtcctcgaag ccgattggta cctcgccgca
      481 gttcctcccc aggagttaac gccccttctg cccgagcgtt ggctctcgcg ttatgcctac
      541 tttcagcaca tcgccgacct tgaaatgagt tcttccacgt ttcttcacgt acgcactgcg
      601 acaatcctga cgaagccacg gcatatcctt ttaagctccg gacccctcca ctggatgatg
      661 tgctcgtcaa gagcaccgga ccggcgcctc ctggccgtca taaaaatcca gcccgaccac
      721 ccagcgcccg atcttgaacg gcacgtttcc acgcttctgg gttcgttaca tctcctggag
      781 cccggccgtc atcccgacgg attgggacag cacgagattc agcatgccgt cctgccactt
      841 aagcccggaa cgaaacttct ccgaccgata cctcggagcc ccattcccaa tctgctcgtg
      901 gccggggcct ggaccgacac gggctggccg gccaatctgg aaagcgcggt tgtcagcggc
      961 gagcgatgcg ccgacattat caccggccgc ctctcagctt aagtgcgggc gattttctgc
     1021 gttgacaact ttttgccccg ctcctaagat gcctcgcgtc gattcatcgt cacggctcac
     1081 catgacgttg caagaactcg cgaaatcgct tcacaactgt cagcagtgca agctcgccaa
     1141 actggggcgc acacaggtgg tatttggggt gggcaatcct cgggccagca tcatgtttgt
     1201 cggcgaagca cccggattta acgaagatca gaagggcgag cctttcgtcg gcgcggccgg
     1261 caaattattg aatgatctac tggcatccgc caatctctcg cgcgatcaaa tctatatcgc
     1321 gaacgtcatc aagtgccggc cgccgaacaa ccgcgatcct gaaccggatg aagtcgagac
     1381 ctgtaagccg tttttgatgc agcagatcca gatgatccga cctaagctgg tgtgcacgct
     1441 tgggaactgg gcgacgcaaa ccttgctcga gcgaaaggtc ggcattacga aggtgaaggc
     1501 ccaagcgttt tacctgaagg actttgtgat tttccccctg ctgcacccgg cggccgcgct
     1561 ccatcaaggc aacctgctcg atacattgaa ggaagatttc agaaagctga aggaattcct
     1621 agaccggcat tcccaaccgc cgcccgagga ggcccctgca ccggctgtat cggcgctcaa
     1681 tatcgaaccg cccaatcccg cccaaatgga cttattcgga tagtggggct gccggcttca
     1741 gcgatgggta ataccgcata ggcatgtcgc cacttggctc cgcaactcgt tccgttcagg
     1801 atttcattcg aacggaactt cgaacaatgc tcgccatcac ttcgcgatcg cagcgacagg
     1861 tctatgcacc catcgcaagt cgccggagcc ccaactatcc tcatcccacg agaaggaaac
     1921 aaaagcgccc atgtctccta tgaatcgctc gtgagaatcg cggcgtcgtc ggtgaccgga
     1981 tgaatcggaa acacaacaag cacaataatc catccgggct tgcagatctc ttctcacgtg
     2041 aagccgcccg cggttgccca tccgtgccct cgcgctctgc atacttgcct tagcgaagtc
     2101 ctgcggcctg cgtcgagcag gtagcgcaga tgtctgagtt ccgtcatcca acgaacagga
     2161 cggaatgagt ttctgcgcta agcaggaatg agcgtgaagg caagccagca agcgccggca
     2221 actgggggca acggcgggcg gctaatctag gctttcgtcg gttttcgttc cggcgatgtt
     2281 gcggaggtag gttctgtgac ttgttcttcg ctctcgcctt caacctgttt gtcttcttcc
     2341 ggcacgtcga accactgcac caacatgtca tcccaccagg cttcgtccac ttccgtgccg
     2401 ggatcgttac caagaaaagc cacgtcatcc ttggtgacat cggctccgac ctgtttgggc
     2461 tgatgcttgg cacgctcgat tacttccttt gtggcatacc cgccgatgat ccaggaatcg
     2521 ggatcggccc gccgggactt gtacgctttc aggccgacct ttaagtacat gaagatttgt
     2581 tgttgaaggg gatcgcccac gcctgattga ggcaagctga gtgtcttttc aatcttctta
     2641 cgccagtggc ggtcgtcgta acgcgaggtc acgagctgca gcattttctt tcccaattcg
     2701 gcatcaagcg gcattgactt cctccatcag cggcgtctct gcgtccctcc gctgctcatc
     2761 ctcgtcgcgg ttatttgcgg ctgtacttgc tcgtgaacga ggccttagcg agcttattct
     2821 gattgaggta atagcccacc atcgcacccg aggcctcttt ctgcagagga agttgatccg
     2881 ctttgagcgc atacactgta aagatatacc gatgcggttt atcaccgggc ggtggacagg
     2941 gaccgccgta tcccggctga ccgaaatcag tcatggattg gatgctgcct tgcggcgctg
     3001 ctccgctctc cggcttaccg gcaccggctg gcagggatgt cgtatccgga gggatattga
     3061 agatcaccca atgccaccac ccgcttccgg tcggcgcatc cggatcgtag accgtcaccg
     3121 cgaaactctt cgtccctttc ggtgccctct cccatcgtaa ttctggcgac gtgttgttgc
     3181 cggtgcatcc gaatccgtta aagacatgtt catgaccgat ggtccctttg tctttcacgg
     3241 tgggactgct gaggcgaaag tcggcggcca tgccggcggt cggaaacagc agcgcagcca
     3301 agagcatcat ctgacatgca cgtcgcataa cccactcctc tctgtggatc tcgtgacccg
     3361 cttgcagccg cgcggctgcc cggatgactg cactgagtac gatcacgtga aacttcatcc
     3421 acccttctgg tacgtcccga ttaactgcgc ctcgcttaag atgtggcctt tcatcgcttc
     3481 ctcaagcttg ctctttgccg gctgcttcaa gtcgggcagc agggtatcca acgcatagag
     3541 cttatggaag taccggtggc gcccgatcgg cgggcacggc cctccataac ccgtgcgctt
     3601 ccaatcattg accccttctc tggttccctc cggtagtgtc ttcacgcgtt ccggcaaacc
     3661 gttggccgtc ggcggcaaat tgtacagcac ccaatgcacc caggtcattt tcggtgcggc
     3721 cggatcgggt gcgtcagggt catccacaat caacaccaaa cttttcgtac caggcggcac
     3781 gtctgaccag gccatcggcg gtgagacatc ctggccttcg catgtgtaca acaccggaat
     3841 tgctccctga ttagtaaagg cgctcgaggt aatgtgcata ttgccctccc cgcgggctga
     3901 gacggccatg gcaggaagaa taagggcgag gggcgctacc agcaaggaat gacgaactcc
     3961 tgcagacaag cgtccgaagg gtctaatgct cttcactatg cttgctcagc tacccgcgac
     4021 catcatctct gcgatcttga gcgtcggact agcaatgcgt cctcggaaca caagatcgct
     4081 gccaaccgat tcgatgtcca tgaacatccg tttcaaattt ccggctatcg taatctcttc
     4141 aacgggataa gccagttggc cattttcgat ccagaacccg ccggcaccgc gcgaataatc
     4201 accggttacc atgttaatgc cgaacccgat caactccgtg acgtagaggc cgtccttcac
     4261 gctccggata atgtcttctg ccggcgtcgt accgggcacg agatagaaat ttgtcggtcc
     4321 gacagaagga ctctccccca cgctgcgcga tgcattaccg gtcgagggca gccccaactt
     4381 tttcccggaa taggtgtcga gcaggtaact tgccagcatg cctttgtcga tgatcgtgtt
     4441 ccttcgggtc ggcagtcctt ccccatcgaa tggccgtgag ccaagccctc cgatcatgcg
     4501 gccatcgtca acgaccgtga tccagtccgg cgccacctgt tgtcccagct gccccgcaag
     4561 atacgatgca cccttgtaca ggccgtaccc agacgcggca ctacagagat taccgagcag
     4621 gctgccagca gtttcgcgat cgaatatgac gggaacgcgc ttcgtggcga tttttcgtgc
     4681 gcccaatcgt ctgaccgtcc gctttgcggc ttcaatccca atagcttccg gcgtttccag
     4741 cttgatgaac tttcgctgga cgtcgtacca ggcgtcgcgc tgcatggcgc cggacgccgg
     4801 atcgactgcc accggtgaga cagccaatga atagctcgaa ctgcgatact ctcccacaaa
     4861 cccgtgactg tttccgagca cgacgcgacc ccatgaagaa tcaaaatccg cgccctcgga
     4921 attcgcaatt cgttcgtctg tcgccatggc cgccgactct gcccgcctcg ccagctcgat
     4981 ctggcgttcg gcatccacct tcgtgtcatc ataaatatcc aggtccggtt gttcctgcgc
     5041 cagctgctca ggttcaggca gccccgacat ggaatcctcg acgacagccc gggcgagcgt
     5101 acaggtttct gacaccagac gtcgcaacga ctcggaggag aaatcggatg tggaggtcgt
     5161 ggcggagcgc ttgccgacga agacgcgaag ccctagccgt ttttcacgtg ccttcgtgag
     5221 ccgatcgaca gccccgagcc ggacctgcac cgacaggttt tccccgtcgg cgacgactac
     5281 atcagcttcc gtcgcgccgt cttttttcgc catggccaag acatcggcgg cgagctggag
     5341 caggtgcccg ttcgcaggct ggatcgcgcg atctgggttc tcttggtgca tcacaaacca
     5401 ccttgcttca tgctgcctga gttcctccaa ccgtgatctg atcgatccga atggtgggca
     5461 atccgacgcc gacgggcacc gattggccct ccttgccgca ggtgccgatg ccttcatcga
     5521 gtttcaaatc gtggccgacc atcgagacct tcgtcagaat atcgggaccg ctgccgatca
     5581 gtgtcgcgcc cttgacgggc ctcgtgatct tcccgccttc gatcaaatac gcttcactgg
     5641 cggagaacac aaacttcccg ttggtgatat caacctgccc tcccccaaac gagacggcat
     5701 acagcccttt gtcgaccgat cggataatct cgtcgggatc cgactgtccc gccagcatga
     5761 aggtattggt catacgcggc aggacgatgc tttggtagct ttcgcgccga ccgttaccgg
     5821 tcaccggaat tcccatcaaa tgcgcattca acctgtccgt gatgtagccc cgcaagatgc
     5881 ccttctcgat gagggtcgtc cggctggtcg gcgtgccttc gtcatccata ttcaaggacc
     5941 cacgccggcc gggcaacgtc ccatcatcca cgatggtaca gacctcggag gctacccgtt
     6001 tgccgatcaa gttggaaaac gccgatgtct tcttgcggtt gaaatccgcc tccaggccat
     6061 ggccaatggc ttcgtgcagg agaatgccag gccagccgcc gcccagcacg accggcatga
     6121 ctcccgccgg agcgtcgacc gacgaaaggt tgaggatggc gatgcgggca gcctctttcg
     6181 catagtccaa gtaacggtcc cgctcgcgat aaaattcaaa cccgatgcgg ccgccgcccc
     6241 caaaagagcc aatttggcgg ttgccgtgtt cttcagcgat gcaagtcacc tgcaatctgg
     6301 acaagggctg gacatcgccg atcaacgtcc cttcagacgt ggccaccatc acgatcttat
     6361 actcggtgtt aaaggaagcc atcacgtttt tgatgcgcgg atcgtaccgg cgtgcttcct
     6421 cgtcgatacg gttgagcagc gccacgcgct ccgctgtcgc cacttccagc tgtgaatggg
     6481 aaaccggata caaatcacgc gtcggcctgg aacgcaccgg cacggggacg ggttcagtgc
     6541 cgccgggaga gtgggcgatg tagcgggcgg tatccgcagc aagctcgagg tcccgcttcg
     6601 tcagttcatc ggaataggca aacccggttt tttctccggc tgtcgcgcgg acacccaccc
     6661 cttgggaaat actcttcgtc gcccgcttca cgagcgactc ttccatcgat acggattccg
     6721 aaacgcacga ctcgaaatag aggtccgcat aatccacatt gcgaaccttg acccggccca
     6781 aagccccctc gacatcgcgc tcagtaacgc cgaatgaatc caatggaata agttgtgcca
     6841 tcgcgcaaga atccttagaa gatggtttta aaattgatcc atgaatgcga gaagtcagag
     6901 tatagccgat tcaattttcc aggcgcaatc agcgcgcgtc gaactcttcg cttttccaag
     6961 gattttagga aatatcgttt gactttcaca gagaccgtca gtagactttc ctcacctgcc
     7021 attcgcacgc catagaaagg accatatcca tacagagctc atggacactc agccaccttc
     7081 cagaattgac gatctgtcgg aaacagaact gacggcaaaa ctcgatcctg accttcttcc
     7141 caaacacgtc gccatcatca tggacggcaa cggtcgctgg gccgaggctc gaagtttgcc
     7201 caggattgcc ggccaccgcg agggcatcaa gtcggtccgc gacatgattt ccttatgtct
     7261 ggagttgggc attcaggctt tgactattta tgcgttttcg caggaaaact ggaacaggcc
     7321 tacgacggaa atcgacggcc tcatggggct gttggaacat tacctgtcaa cagagcgaaa
     7381 taagttgatc gaacagggag ttcgttttcg cacgatcgga aggaccgagg ccctgccgac
     7441 ttctgccctg cagtgggtgc gcgcggctga acgggaaacg gcacgtatgg agaagctgca
     7501 actgacggtg gcgctcagct acggagggcg cgctgagatc gtcgatgcgg tccgaggact
     7561 ggttcgggac gcccaggagg gcaaactgca gcctgaggat atcgatgaac cacagattca
     7621 acaatacttg tatacgcgga gtctgcccga tccggacctg ttaatccgca cgagcgggga
     7681 aacacgcatc agcaactttc tcctatggca gttggcttac actgaattgt atttcacacc
     7741 aaccctctgg ccagacttcc gccgccggga gctattattg gccctactcg aatatcaaag
     7801 gcgcgaacgg cgtttcggcc gcgtcttcag cagcctgtct tcgtagtctg ccggagaatc
     7861 accgacatgt tggagtgcgg aacaacgaat cgggactcgg cgcataccgt ccacaaggcc
     7921 gcgggtcgat tcgatgcccg tcggatctac acagtcctgt tcgtcgttcc cctcttgtat
     7981 acggccatcc gcttttttcc tcccgctgtg tttaccggcc ttgtggcggt ggccggctgt
     8041 ctcgctctcc gtgagttata tcgcttgtgt ttccaggttg tacgtcaacc attgatggtc
     8101 ggaatcggcc tggcagggtg tgtcgcgttg atcgtcagtc ctcacgtacc tgctctggca
     8161 cagccgggtc tgctcgtatc cctcgtcgcc atcctctcca tcccgctgct gatcagaacc
     8221 cccttggatc aaagcgtaaa ggatgctgcc ctcactttga ccggcatgat gtacatcggc
     8281 ctgatgctgg gctacctggt cgcagtccgg ctgcttccgc aaggcgagtg gctgctcttc
     8341 tttctgctct tggtcacgtg ggctgccgac acgggcgctt actacgtggg tacgctgtac
     8401 ggtcagcgcc cgctggcccc gcggatcagt ccgaagaaaa cgatcgaagg tctggcaggg
     8461 ggcctatttg ctgcagtgct gatcgccttt gttgcacgat gggggttcgt gtcggacttc
     8521 tcccattggg atggtctcat actcgccgtg ctcttgacgt tcgccggctt gtggggagac
     8581 ttggtggaat cggccatcaa acgcagtgtc ggcgtgaaag attccggggg gctgttgccg
     8641 ggtcatggcg gcatgctcga tcggctcgat agtctcttgt tcaccgcgcc cacgttctac
     8701 tactatgtca cgtcgattcg tgacatcgca ccgtcgccat aaaccaggtg tcaggaagag
     8761 aggatctgtt gcaggatgtt cacaatcagc gcccgacgag tgaagaagcg agtcatattt
     8821 ttacgtccgg gcttctcgac gatgtgagaa cgaagcccgc ggtcaggcga gtcccgggag
     8881 atcgattgcg aagcaatcgg tgggtcgggc gggtgagacg acatgttcaa catcctgcct
     8941 agggagcggt tatgaaatct atcgtgatcc tggggtcgac cggatccatc gggaccaata
     9001 cgctggacat cgtggagcga ttccctgagg aattccgcgt cgtgggcctc acggccggga
     9061 acaatgacga aatgttggcc gagcagatcc gccgttttca cccactggcc gtggccatgc
     9121 cgaccgaatc aggcgcggcc cggcttaagc aacggtgcgg cggtcagccc gtcgagatcc
     9181 tgagcggacc ggaaggcttg gccgccgtgg cctcgcttcc ggaagccgaa ctggtggtat
     9241 cggccattgt cggaggcgcc ggcctcgttc cgaccctggc cgcgatccgc agcaagaagc
     9301 acattgccct ggccaacaaa gagccaatgg tgatggccgg ccagttgatg caggaagaag
     9361 ccaggcgtca aggcgtcaga attttccccg tcgacagcga gcacagcgcg atctttcaat
     9421 ccttggaggg ccatcgcatc aaagacgttc gccgtctgat tttaaccgcc tcaggagggg
     9481 cgctgtggaa cgtgccgaag gagcaactcc aggacgtgac gccggagcgg gcgttgcagc
     9541 atccgaattg gaagatgggt gccaaaatca ccatcgactc ggctaccttg atgaacaaag
     9601 gtctcgaggt ggtggaagcc cgctggttgt tcgatatccc ggaatcgagg atcgacgtct
     9661 tgatccaccg ggaaagcatc attcactctc tggtagaata cgaagaccgc tcaatgattt
     9721 cacaattagg cttaccagac atgcgcacac cgatctccta tgcgatgcga tatcccgacc
     9781 ggcttccgct cgacttgccg tcgttggatc tgaccgaaat cggcacgctc acgttctgca
     9841 agcccgacca cgatcgcttc ccctgcctgc ggctgggcta tgagtctctc cgtatcggcg
     9901 gcaccatgcc ggccgcgatg aacgccgcca atgaaatcgc ggtcgatgca tttttgaacg
     9961 gtggcatccg ttttaccgat atttctgata tcattcagag caccatgcag acgcatgtcc
    10021 cgcaagccgt ccgctcccta gaggcggcct tggaggctga tcggtgggct cgggagaagg
    10081 ccgactcact ggtccatgca ctggcccgct gacagggaac gaagaacggc attttagcta
    10141 acccccaacc atcgagtatg aatatgatgg catcggtttt ggcttggtct cccgatagtg
    10201 tctggcttct gttgcagaag acctggtggt tcctcgtggt cctcggcatt ctcgtcgcat
    10261 tccatgaact cggccatttt ctggctgcgc gctgggtcgg cgtcaaggtg ctgaagtttt
    10321 ccttggggtt tgggccgaag ctgctcggtc gccaggtggg tgagaccgag tatctcgtat
    10381 ccgcaattcc gttgggcggc tacgtcaagc ttttcggcga ggaggagacg gaagccatca
    10441 cgcccgagga tcgccggcgg tctttttcgc accagagcct ttgggggaaa gtcctgattg
    10501 tctctgcagg cccgggattc aatttcatct tagcctactt gatctttgcc gcttggctgg
    10561 caaccggtta caccttgccc gtcccctcat ttaaggacat cgctcccgtt ctggaagccg
    10621 ttgcgcccga ctcgccggca gccagggccg gcctcatggt cggcgaccaa atcaagaaga
    10681 tcgacgggcg ggacattgcc acgcgcgtgg aactgctcga tgccgccgcc aaagggaaag
    10741 gcgcgccgct gacactcgag gttcttcgag acggccgcat ccaaaccctc acggtcacgc
    10801 cgattccggc gccggcgccg actcaacgga acgacgactc aggctattac ctgggaatcg
    10861 aagagattcc accggtcgtc aatgaagtgg taccaggcaa acctgctgcc gctgccggat
    10921 tcagggaagg agaccgggtg ctttcgattg atggtcgcac gatccacacc tggacgcaga
    10981 tgacgggtct cgtcaaagac agcccgggca agacgcttca ggtggatgtc ttgcgcgacg
    11041 gccaccgggt atcgttgtcg gtgacgcctc tcgctgaaag cgcgacggtc aacggtcagg
    11101 cggttcaagt cgggaagatc ggcattgccg ggccggatcg atcgctcatg cgatcggcga
    11161 atccgctgat ggcggtgtac aacggcctcg aagccacatg gaagtggacg gaactcacga
    11221 tcgtcgggct gtacaagatg atcgttggcg atatctcgag caaaaacatc ggtggcccat
    11281 tgaccatcgc gaaaatttcc ggagaagccg ccgcccaagg tccggccaat gtaatttttt
    11341 tgatcgcgct cctcagcatc aacctgggtg tcctgaatct gctgcctatc cccattctcg
    11401 atggcggtca cttgcttttc ttcctgatcg aaggcatcct gcgcaaaccg ttgggagagc
    11461 gacagcgtga gctcgcgcaa caggtcggcc tggttctgct cgtgggcgtc atgatctttg
    11521 ccttttggaa cgacctcgaa cgtatcttct tctctcactg acattcatgc ggacttctca
    11581 actcctgctt cccaccctgc gggaagatcc gggtgaagcc gaaaccatca gccaccggct
    11641 tatgcttcgc tcgggattga ttcggaaggt cgccgcaggc atctatacgt acctgccgct
    11701 cggtctgcgg atcattcgga aagtcgagca catcgtccgc gaggaaatga accgggcggg
    11761 ggcgcaggaa ctgttgatgc ccatcgcctc tcccgccgag ctctggagag aaaccggccg
    11821 ctgggatttt tacggcaagg aactgctgcg gttcaaagac cgtcacgagc gggatttttg
    11881 tctcggcccc actcacgagg aggtgatcac cgatctcttc aggcgtgagg tccggtctta
    11941 ccgtcaatta ccactgaact tttatcagat tcaaacgaaa tttcgcgatg aaatcaggcc
    12001 gcgcttcgga cttatgcgcg gccgcgaatt catcatgaag gacgcttaca gcttcgataa
    12061 agatgacgtc ggcgccagaa ccagctatca gaagatgtac gacgcctatc accgcatctt
    12121 ttcccgctgc ggtctcaatt tcagagccgt ggaagccgat acagggttga tcggcgggac
    12181 ctcatcccac gaattcatgg tactggccga taccggagag gaaaccgtcg tctacagcga
    12241 atcgggaacc tatgccgcca acgtcgagcg cgccgaagct cttcccccga tcgagacaag
    12301 cagggaagag cttcgcccac tggagactgt cagcacccct ggccgccgca ccgttgagga
    12361 ggtgacggca ttcttgaagg tcgaggcctc caagctcgtc aagacgttgc tctattccac
    12421 cggaaccgaa accgtggccg tgttgattcg aggcgaccac gatgcgaatg agatcaagat
    12481 caaacgtctg ctgggcgtcg cccagttgga gctggccggt cccgcgaccg tggaaaaggt
    12541 caccggtgca ccggcgggat tcgcaggtcc ggtcgggctc aaattgcgca ttgtggcaga
    12601 tcacgccgtc aaatcggcca caaacgtcgt cgtcggaggg aatcgcaccg atacgcatct
    12661 gctgaatgcc aacgttgacc gcgattttca ggtggaacaa ttcgcagatt tgagaaatgc
    12721 ccgggccggt gatccatctc cgcggaaaga cggcgtgctc aaagccacga aaggcatcga
    12781 ggtgggccat gtcttcatgt tggggaccaa gtacagccag agcatgaatg cctcctttct
    12841 cgatcccaac ggcaaagaat gcctggcggt catgggatgt tatggaatcg gaatcgggag
    12901 gacggcggcc gctgccgtgg aacaacatca cgacgacaag ggcattatct ggccctatcc
    12961 gatcgccccc tttcatgtgc acctcattcc attgggccaa tcaggtggcg ttgccgaggc
    13021 taccacctct ctgtaccatg cgctggagac ggcaggtttg gaagtcttgt gggacgatcg
    13081 cgacgaacgc gccggggtca agttcaacga cgccgacttg atcggagccc cttttcaagt
    13141 catcgtcggc gataagggtc tgactgaagg cctcttagag gtaaaaacca gaaaaaccgg
    13201 tcacaagacc aaacttgccc ccgcggaaat ccctgcgaga attctgcaga tggcctctca
    13261 accctcttga acgagcatcg cttcgatgcg cgttctcccc gttccctcaa gtcgttcgcc
    13321 gtcgcgcatc gtgaaagcgc cttttccttg ccttcccatc ctgttgggct agactgagtc
    13381 tcgacttatc actgtgaggc aattgagcca cggctgccaa cggccgccgg cctgaccgtc
    13441 ctccatgtgg tccgcgttcg ttacaggagc tgagtgatgc cgacccaacc gccgacacag
    13501 aacctgcagg agctgcaaca aaaagttgaa ttcgcggaac acgtcaaacg catcacgaat
    13561 cagattcacg ccgccagcaa tctcgaccag attctgctcg accttcacaa agacattctg
    13621 agcctgttcg atgcggagga tctgaccctc ttcgcctttg atgccgacaa gaaggagatc
    13681 ttctacaagg ttccgcacat cgataccatt gaagaagttc gcattccgat caccgagcag
    13741 agcctggccg gattctgtgc gaagtatttg cgcccggtga atatcgccga cgcctataac
    13801 gtggctgaac tgcaagggat ccacccttcg ttgaatcacg atacctcgta cgacaagcgc
    13861 acgggattta agaccagaca ggtgctgacc tatccgatcg tcgccgacaa caagtacctg
    13921 atgggtgtgc tccaactcct caacaagaag agcgggctgc gctttacccg caaggacgag
    13981 gaatccgtag ccgaaatcgc caaggcgctc ggcattgcat tcttcaacct caaaaaggtt
    14041 tccacaaaga aagcctcgac caagttcgat cttttggtga ccaacggtaa gatcacgcag
    14101 aacgaactcg ataacgccat cgccgaagcg agaaaaagca tctcctccga ccttgaatcg
    14161 atcctcatgg aaaagtacaa ggtgccgaag ctcgagatcg gcagatccat cgcccagttt
    14221 cacaagtgtc cctacatcga atacagcgaa cgcacactgg tcgacatcga gctgctcaga
    14281 aatctcaacg ttgattacct caaaaagaac cactggatgc cgttgaaacg cgaccgcaca
    14341 gcgatcgaaa tcctcaccga cgaccccggc gatctcgatc gagtcgcgga catcaaacgg
    14401 acattccccg gtcttaacat ccgtttcgcc gtcagcctca gacgggacat ctcacaattc
    14461 ctgaactctg caaccggcgg cagcgacggg caaccgggac gaaaactcga tgaaaacgtg
    14521 tccgacattc tcggcgagct cgtgacggaa gctcaggccg aggccatgga agagtcgggt
    14581 gccggcggag ggctcgacga gaacgatagc gcgatcgtcc gtttggcgaa tcagatcatc
    14641 gcggacgcgt atcggcagac tgcctcagac attcatatcg agccgtacgg agaaaaacga
    14701 gaaaccctcg tgcggttccg ggtcgacggt gattgtttcg aatatatgaa gatcccgcaa
    14761 agctatcggc gcgccatcgt gtcacgcctg aagatcatgg ccagccttga catcgctgag
    14821 cggcgaaagc cccaggacgg caagattaaa ttcaagctca gcgagacgcg ggaaatcgag
    14881 cttcgcgtgg cgaccatacc aacggccggg tacaacgaag acgtggtgat gcgtatcctt
    14941 gcggccagcg agccgctgcc cctcgataag atgggatttt ctgatcggaa tctgaagcac
    15001 atcaaggaca tcgccgagaa gccgtacggt atcattctct gcgtaggacc gaccgggtcc
    15061 ggtaagacaa ccacgctcca ctcggttctc gggttcatca acacccccga catcaagatt
    15121 tggacggctg aagaccccgt cgaaatcacg caatacggac tccggcaggt tcaagtacag
    15181 cccaaaatcg attttacctt cgcgaaagcg atgcgcgcgt tccttcgagc cgatccggac
    15241 gtgattatgg tcggagaaat gcgggataag gaaacggccg agatcggcat tgaagcgtcg
    15301 ctcaccggac acctggtctt gagtacactg cacaccaaca gtgccgtgga aacggtcacc
    15361 cgtctgctcg acatgggctg cgattccttc agcttcgccg atgccatgct cggtgtgctg
    15421 gcgcaacgat tggcgcgaag ggtctgtaaa gactgcaagg agcaatatac gggcacgaag
    15481 gaagagtatg aggaaatgcg gctaggctac ggacctgaat tttgggacaa actcgccatc
    15541 ccgatggaca atacgttccg cctctcacga gggaaaggct gcgaaacctg taaccggacc
    15601 ggcttcaaag gtcgggttgc attgcacgaa ttgcttttag gcaccgacaa gctgaagcgg
    15661 ctcatacaga ccagggccaa gactgaagag atggtcaggg ccgccatcga agacggtatg
    15721 accacgctca tgcaggacgg cattcagaaa tgcttgcagg gactcacgac gttcaaagaa
    15781 gtgaaagccg tcgccatcaa gtaaggagag gatctcaact ccacggttga gatcctcttc
    15841 cattcccctc cgggcgaact acacacatga cacgaaccgg tctcgtcctc acaggacggg
    15901 accatcgctg acccggtgta ggcgaacgtc aggagcaggt gtatgccgag cgagaaagga
    15961 atttcttgag gcgagactct accgacagag ggacagatag gaattccagt ccgaatagct
    16021 gatcattgac ccatttgacg gtcgcgtcga gctgcagggc ggaggcttgg tcaggcattc
    16081 gaaagctgac cgccagatgg tcgcccggtc tcattgccgc ggcactcatg actttcgcgc
    16141 cacccaacga gacgtccagt acaacgccca gcccgttgcg gtcaacggcg gcccagcgtt
    16201 ttagcccgag gagggagaat gcacacggga agggagcgga gactcgatga cgaggatagc
    16261 gtcgcagctc cacgctcggc tgaggctgtc gttccagttc agccatggaa ggttatgcga
    16321 ccgctcgcga ggcgatcgcc acgaatctct tcaatcgatc atacgcatcg agcgagaggc
    16381 ggcgaaatgc cagcccatag aactgatcct tcgtccatcg cacggttgcg acggcaatct
    16441 ccgcggaacg gatttgcttc ggcagacgaa ggctcaaggc cacctgatcc ccgggcttga
    16501 agggagcttc cgtgctgacc cgcgcgcccc gaacagaaag atcatagacc aatcccacat
    16561 tgatgaccgg ctttctcagc cagcttcgcg actggaccgg agagagggca caagcaaacg
    16621 gcgtgttgat tcggactctt ggatggcgtc gaaagcgatt caaggcagac ggtcgacgcc
    16681 catccagccc tacggggtgt ccttgcatac ggctactcta caacagggat ggatggatcg
    16741 aaaaatgatt tcgaagaaag tacagtcggc ctagaaggta gacggccatc ctgcttcggc
    16801 gatgagtgga aggaccgacc acaggcccct atcggacctg tggtcccggg ttcctacagt
    16861 ggtcacgacg ccatgcgata cacccacctg ccggtatttc gtcttgcctc agtcaaggct
    16921 ttggcttcac gggtggcggc agaaagcatg agccctccga agaagatgct gaacagcgca
    16981 agggcggctg ccatcaggta gcccattgga atgtcgggaa tgcccggcat gccggctgaa
    17041 ctcctcgcca cttccacctg cccggccacg gcagaaactg gtcgcgtgac agcctcgctc
    17101 atcccgccgg tgcgatcgag tgcggccatg aggctgccga gttgatattg attggacgac
    17161 caggcttcgt ccaaaagcat tctcgcttgg gccacatgga gaatggccga ccccaactgc
    17221 tcttgcacta cctcgacgcg ttgaagatgt tcttgactgg cgtccacgat gcttttaccg
    17281 agtagggatt gccaggttga ggcaaagccc tcgtgcatcc gctttcctcg ttgttctgtg
    17341 gcgccgatca tcctctggtt gtaatccgaa agatacaaat ccgcggagaa gacgccgctt
    17401 cgaatgcccc gctgtgtgaa attcacgatc gagcgtccca tcacggtttg cactcgggcc
    17461 tcatgctcgc gtggaatgtc cttcgcgcgg ctgatgacca tggcaaacgg actatcgatg
    17521 acggtatgca ggctgcgatg cgactgcaga gcctgtctcc attcggacgt ggccgaagcc
    17581 gtcatgcgat cagtctgtat ctggagcagc gcccgctcga caatggcctg ccctaaagca
    17641 ggctgaagcc atgccgactc agtagtgacg tgagagcctt cggccatcgc cgttggcgga
    17701 ttgcccacaa gaaatgttcc cgtggtcgcg acaaacatca acaatgcacc gaagactatg
    17761 gcgcacacac caacgcctac aatgatatcg acgctcgaat atcggtagga catgagatcc
    17821 tcctttccgc gtcgggcaag acctcagcct ccggcatgag ggatacctgg ccgactcggt
    17881 catgcatggt gatactgcga tgcgcttaag tcgaacgaaa ggatggcgca gcaacggcga
    17941 tccacggcgg acggaaggca atatgatgac agagatcccg tctggtgaga agcaccgggc
    18001 aggtccgggc cgtcggttct gaatcccacg gaggaccatc ggggcaccga aatggtatgt
    18061 gacggcaaga aaactatgta aagcctacgc cccgggcgca tgaagcgtca aggggaagac
    18121 tcaccggacc cacggtggcg gagccgtgag tggaggcgtc agtctctcaa tcggaccaga
    18181 ggaacggccc ggaggctccg gtccgatcgg tgatccctcc ggacgggcat cccgcttcaa
    18241 ccgaacgacg agccccccca taacgtccca tcgttgaaaa tcacgtctgc tggctttggg
    18301 gtggtggttg tggcaatcca cacaggactg cacgagggca agatccgctg atatcgcctt
    18361 gaactgatct ccatcgacga acgtcgtgac ggaccgctca cggttcttca tgagttggat
    18421 caaggcatcg ctctccgctt gagtccgggg atagttcgct tgattgacgg gcgtcaatcc
    18481 aatcagaccg atctcaaaat tgtcgagctg gtctgcagcg gctttgacaa attgagcagg
    18541 cagtggaatg aagtgcgcgt cgctctgcca attttcattc ggtttcacgc ccccgtccct
    18601 tgcatgctca acgactttga gcacgtaggc cgtccggaac gctttcacga cctctaaaat
    18661 gtatcgagca gtaagttcaa atgagggttc cgccgccttg actgtgtacg ccgctagaca
    18721 acccgccaac atcgcaatgc aaacggacat cgtacgcggc ttcataccca gctcctctca
    18781 aaaaagtgca gggtagcttg cgctcacgcg gtgggtcaag gggtcataac ttcccccgca
    18841 ggggtgtttc ctagctaggg gaagctcgga acagcggtag gactttagaa gagacgtttg
    18901 aaaaggaata ccttaaagga gctcacatgt ggatccgggc agtcctctgt gcccttctct
    18961 gtctgctgac agcgtgcggc ggttcagata gctcatctcc ttcgacatcc gaagcaccgc
    19021 cgccaggtca gcaacctccg ccaggtcagc agcctccgcc gacttcgggt ccagccggcc
    19081 cgtggccgct ggctgatctc acggtctaca gtgcggctca aagcctgacg ggcaccatta
    19141 tcgatgcaag tccagacgat gcccagaata tttgggcggt gacgccggat gccctgtacg
    19201 tcctccgacc cggagaggcg acgttcaggc ggtttaccgc ggctgacggt ctccacgtcc
    19261 aaacctatac cgatccggcc aatggccagc aaacggtgag caacatcacc gcgatggcgg
    19321 gcggaatctc caatgaggtg ttcgtcggct acagaggtta tgaagcacct attccgcctc
    19381 cggcgccacc accctgctgt gtcgcaaatg cggatttcag cgatcctcgt tggcacctcg
    19441 gccaggcgga caaggtgacg ctcgatcccg gcggggggat tcaagttcgg cggtatgagt
    19501 ttctctgcga tatcaccgtc aattgctggg aagaacgctc tgcacggcgg atgatctttg
    19561 ggcataatgg taccgcagct ggacatttgt tcattggatt cgatcatggc gttgcacacg
    19621 tgttcaacga cgtgttcggg gatcacacgc atatcgtgac attctggcat tacgccgacg
    19681 gcagaaacgt catgcgaatg ggcgagcact atggtctggc tctgtatccg aatggtgaaa
    19741 tgatcgacgg caccggatat ggcatcggtt cacatgagtg gaatgctgat ccacgagcct
    19801 gggtcaacgg tcggtttgtc tggggcttca ccacctatgg gccggccgaa ccgtataaca
    19861 atggagaaca tgatttgatc gtcccggaag gctaccgcga agataaccgg ggtgcggcag
    19921 tgacgccgga tggcacggca tggtgggtca gtcttcacac cggattatcg agctacaatc
    19981 acacgatcgc tcaaggaaat acgtcgttga ttcgccacta cacggatgtg gcgggattgc
    20041 ctgctgccgg tctcatggac atcgctgccg atccggatgg atcgctttgg atcgtcgata
    20101 acagcggacg cctgttgcgc ttcactccct cgactcaagc cgtgcaggtc tggcccggag
    20161 tctccagcgt gcgccgcatc gtgatggata cgactgttac tcctcgtgcc ctctatgttt
    20221 caatgggcag ccaaggtttg gcggtaatcc gggcaagata acaccatccc cacgctcctg
    20281 ctctcttcga agcaggtggt agtcttcatc gactactacc tgcttacttg cttgctcaca
    20341 gacattctcc gtactttctc cgccatcacg catgacgttc atcctcctca tcgccgttga
    20401 acctcttatt gcttgcatcg cgatattctc tctggtacaa gaacctacaa aaaaaccggc
    20461 acccttattg aaagaaggtg tacatgattc ggtatctcat gctgtttctg atcatgattg
    20521 cgctgagcca ttgcaccagc gtcaaataca gtgaaacgga ctgtggcagc cttccaagcg
    20581 acaccttttg gtccggcaac gtcgccctgc aatgccaaga atat
//
LOCUS       DASSCQ010000172         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_644000, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000172 DASSCQ010000000
VERSION     DASSCQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644000"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFH05_11595"
     CDS             <1..690
                     /locus_tag="VFH05_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6678908.1"
                     /translation="AAIGAFWYFSSTDPRVGDQLDSSAVTEMLKDNQPVGMSVPVANL
                     QTAAAVATPQPGAIHADLYFEVGRKGLSDEARKELQEEAALLKGDENLGVLIQGYTDQ
                     QGSAGFNLALGRKRAEAVKTELVRAGVADHQIKVMSLGKEGALCIDSSDVCRRMNRRV
                     HLEIRPIGREHMMPPAAAVTTQAGVAPASADSDKETGDAGVLTDSVLPAANASPDTHN
                     GPSALEPASGS"
     gene            complement(796..1239)
                     /locus_tag="VFH05_11600"
     CDS             complement(796..1239)
                     /locus_tag="VFH05_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678909.1"
                     /translation="MLVLPGGRMEQWIPRDALMWVSVGSGVALVIGAIAVPWIVINLP
                     QDSFSNPRRPRWLDQQPIAIRLPVRLIKNLLAGALIVLGIVMLVLPGQGIFAIVLGVL
                     LADIPGKLKLQQRIVGRQNVMNSLNWLRRKFGRPPLERPSVQTPA"
     gene            complement(1352..1849)
                     /locus_tag="VFH05_11605"
     CDS             complement(1352..1849)
                     /locus_tag="VFH05_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6678910.1"
                     /translation="MTAAQKIRVLIVDDHAMVRQGLRAVLQSYPNIEVVGEAANGEEG
                     VACVQKLQPAVIVMDIGMPNLDGIGATRLIKIQYPQIAVVGLSVNAQSFHVDAMLKAG
                     AFEVLTKDKAVDELYSAIQRAIAAVQPILILEEKPATAGSTAETKQTEKRAPSDVTRS
                     QEHNR"
     gene            complement(2231..>2692)
                     /locus_tag="VFH05_11610"
     CDS             complement(2231..>2692)
                     /locus_tag="VFH05_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6678911.1"
                     /translation="FDTTDGRARKVNWQMTPLSVQSSPPSKTIVLIEDNEEDLQYWSK
                     TLRSAPFNYTVLETSDAQSGLELCRERAIDCVLLDLDMPESGFHVLLELIPNRSHPRI
                     PVIILTHLPHPNLWEMAKHNGAQACLLKQRTSAQELHCAIQTAITSVKSQN"
BASE COUNT          582 a          756 c          788 g          566 t
ORIGIN      
        1 gccgcgatcg gcgccttttg gtacttctct tcgaccgacc cccgtgtggg agatcagtta
       61 gacagttcag cggtcacgga gatgctcaag gataatcaac cggtgggcat gtccgtgccg
      121 gtagccaacc tgcaaaccgc cgccgctgtg gccacgcctc aaccgggcgc gatccacgct
      181 gatctctact tcgaagtggg ccggaaaggc ttgagcgacg aagccaggaa agagctgcaa
      241 gaagaggcag cgcttctcaa gggcgatgag aatttggggg tgttgatcca aggttatacg
      301 gatcagcagg gctccgcagg ctttaacctc gcgctcggcc gtaagcgggc agaagcggtc
      361 aagaccgagc tggtccgtgc cggtgtggcc gaccatcaaa tcaaggtgat gagcctcggc
      421 aaagaggggg cactctgcat tgacagcagt gatgtctgcc gtcggatgaa tcgccgtgtg
      481 catctggaaa tccgtccgat cgggcgggag cacatgatgc caccggccgc cgccgtaacg
      541 acgcaggcgg gtgttgcccc ggcatccgca gattcggaca aggaaaccgg cgacgccggc
      601 gtgctcaccg acagtgtgct tcccgctgcc aacgcctctc ctgacactca caacggccct
      661 tccgctctcg aaccggcatc gggaagctga gtctggcagc gttgcgtctg cacgacatcg
      721 ctcgtgcaga cgtcgctgca caccacccac ccgatcatgc aggagcagga gacacgcgag
      781 atggtcagca ggaactcacg cgggcgtttg aacggatggc ctttcgaggg gtggccgccc
      841 aaatttgcgt ctgagccagt tgaggctgtt catcacgttc tggcggccga caattcgctg
      901 ttgtagtttc agtttgccgg gaatgtctgc caacaagacg ccaagcacaa tggcgaaaat
      961 cccttggccg gggaggacaa gcatgacaat gccgagcaca atcagggcac cagctaacag
     1021 attcttgatg agccgaacgg gaaggcgtat cgcgatcggt tgttggtcga gccaccttgg
     1081 gcgtcttgga ttggagaagg agtcttgagg caggttgatc acgatccagg gaacagcgat
     1141 ggcaccgatc accagggcta ctcccgatcc aacggagacc cacatcagcg cgtctcgagg
     1201 aatccattgc tccatgcgtc ctccgggcag caccagcacg accgtcagcc gctcctatgt
     1261 accgtacgta tcggcaacgc tatgaggtac gagggaaagc cccggacaca agctccagaa
     1321 agacttgcgc gatacggtga gtaagcaccc gtcagcgatt atgctcttgg cttctcgtca
     1381 cgtctgaggg cgctcgcttt tccgtctgtt tcgtttcagc ggtagatccg gcggtggcag
     1441 gcttctcttc gagaatcagg atgggttgga cagcggctat tgcgcgctgg atggcactgt
     1501 atagttcatc gacggctttg tccttggtca ggacctcgaa cgcgcctgcc ttgagcatgg
     1561 cgtcaacatg aaagctttga gcgttcaccg ataatcccac caccgcaatc tgagggtatt
     1621 gaattttaat cagcctggtc gctccaatcc cgtcgaggtt gggcatgccg atgtccatca
     1681 cgatgacagc tggctggagc ttttgtacgc acgccactcc ctcttctcca ttggcggctt
     1741 cgcccacgac ctcgatattt ggataagact gcaggaccgc ccgcaatccc tggcgtacca
     1801 tggcgtgatc atcgacgatc aaaacccgaa ttttttgtgc tgcggtcatt tcagagaatg
     1861 atgtcaaact ttccggcttc ggagcgtaat cataagctag aaatgtcgct ctgtctgcat
     1921 gtatctgtca gagaatagcc tacagggcga tatggctgct atcaaccagc agaccgcttt
     1981 cggtcgtccc ttacggtgga agacgcctcg gctgccaggg tgggcagatc tgtcttattc
     2041 cgaagatcta cgcccgtgtg aggaactttg agaacccttc gtctgccaca cgtgtcgcaa
     2101 ggagctcgat gagtggacgc ccgacctgat caagatgcgc taggggcacg atctgacggg
     2161 ttcagcgtgt catggaatcg cagcccgaaa gggtcctacg atcccggcac cggtcactgc
     2221 ctaaaatgcc ctagttctgc gatttgacgg aggtgatcgc cgtctgaatg gcacaatgca
     2281 gctcttgagc cgacgtacgt tgtttgagca ggcacgcctg cgctccatta tgcttggcca
     2341 tttcccagag attggggtgc ggcagatggg tcaggatgat gacagggatc cgagggtgac
     2401 ttcggttcgg aatcaattcc agcaggacat gaaaaccgga ttcaggcata tccaaatcga
     2461 gcagcacaca atcaatggcg cgctctcggc acagctcaag acctgattgc gcatcggacg
     2521 tttcaagcac ggtatagttg aacggggcac tccggagagt tttggaccag tactgcaaat
     2581 cttcttcgtt gtcctcaatg agcacaatgg tcttggacgg agggctcgac tgaactgaaa
     2641 gcggagtcat ctgccaattc acctttctcg cacgtccatc agtcgtgtcg aa
//
LOCUS       DASSCQ010000173         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_644043, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000173 DASSCQ010000000
VERSION     DASSCQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644043"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1129
                     /locus_tag="VFH05_11615"
     CDS             182..1129
                     /locus_tag="VFH05_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET6678912.1"
                     /translation="MATATDLMTTEVPTASEQQTVSETLSALRKRSWDEVGHIYLVDG
                     KRRLVGQVPMERFLVEDGATTLTTLRGAPPIEVQPDEDAETVALRAVVRHDADVTVVD
                     RHHHFLGVIPIGRLLALLHEEHVDNFLRLGGVSERHPHPLAHQDALAAFRARIPWLAI
                     GLIGGFLAGGIATLFEVALRKEIALAFFLPLVVYMADAVGTQTETVLVRALAYGKISI
                     ADQLLREGRVGSMIGGAMGLSAGLGLWIVQGRPAVALVVALTLAVTALVATMVASLLP
                     MGLDRLGVDPALASGPVATVLQDILSVAIYLSIGTTILG"
     gene            1328..1693
                     /locus_tag="VFH05_11620"
     CDS             1328..1693
                     /locus_tag="VFH05_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6678913.1"
                     /translation="MTMPLLARISGLMIASLAVPAMLLAQEYPPDLSRGKAVYERHCL
                     SCHGASGRGDGPAAASLRVAPANFQRFGSFLKSDEELLRTIEHGVVFSPMHSWRGQLT
                     DEEMSDVLSYVRFLSQQAR"
     gene            1772..3193
                     /locus_tag="VFH05_11625"
     CDS             1772..3193
                     /locus_tag="VFH05_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET6678914.1"
                     /translation="MTSAHRVLGQAVVRLGFAVILVGTALTETWPADMSNASGCTSPY
                     KNKAVAANRLRAIVQSHVRWLEQWYEGGQRADLCQSDLRQASLNGANLERANLEGAML
                     RQANLYQSLLTQASLAGADLTSAGLEDSNLSGADARKARLSHANLFRAIGDEAALFDA
                     TLIGAQLHESSFERAHFEGADLTSADFSNANLVEAFFYGANLHRAILSGADLLQADLR
                     RANMTKAILNKTHMQGALLDHAHLSGARLIEADLESAFLDEADLTEASLPDALLRGAD
                     LRFANLHATGLQRADLEGVNFEGARLVKANLQTANLKRAILYHAVFDEADLRDARLHQ
                     AVLIGARGSGAVFTKADLSDVYAPRVVLQNAIFSEAKLESANLVAANLRRANLSSTNL
                     AHANLQETNLQEALCPGADFTGAQMDGADLRLADLRDAKLAEAIGLTQGQLDAACLNE
                     RTTLPPDLTRPPPCTAHNKRRRD"
     gene            complement(3315..3677)
                     /locus_tag="VFH05_11630"
     CDS             complement(3315..3677)
                     /locus_tag="VFH05_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6678915.1"
                     /translation="MFHRNAVVAVITLPVLLAGQPSFIHAGNADMTAGKRIYFELCKT
                     CHGLDGKGPGVMKFNPPAADLTSRQVQAKLDSGLYNSIHEGRANTAMGAWKHALSDQE
                     IRDVVAYIRTFGQGLSAP"
BASE COUNT          816 a         1152 c         1182 g          867 t
ORIGIN      
        1 gcacaggcgg aaggtatgga tctgatcgtc gtctcgaccc aggggcgcca tggattggac
       61 cgcttcctga tggggagcgt ctcgcatgcg atcgtccacc gcgcgtctgg accggtcttg
      121 atcgtccatt gacgggcatc ttcttcgact ggcaggttcc gataatagat gaggcgaggt
      181 gatggcgaca gccactgatc tgatgacaac ggaagtgcct accgcgtctg agcagcagac
      241 ggtgtccgag acgttgtcgg cacttcgcaa gcgatcgtgg gacgaagtcg ggcatatcta
      301 tcttgtggac ggcaagcgtc ggctggtcgg ccaggtgcca atggagcgct tcctcgtcga
      361 ggacggcgcc acgacgctca cgactcttcg aggggcgccg ccaatcgagg tgcagccgga
      421 tgaggatgcc gagacggtgg cgctgagagc cgtcgtccgc catgatgcgg acgtcaccgt
      481 cgtggaccgt caccaccatt tcctgggggt tatccccatc gggcggcttc tagcactctt
      541 gcatgaggag cacgtggaca actttcttcg gctcggcggc gtcagcgaac gtcatcccca
      601 cccactggcc caccaggatg cgttggcggc ctttcgagcc agaattccat ggctagccat
      661 cgggctcatt ggcggatttt tggcgggagg aatagcgacc cttttcgagg tggcactcag
      721 gaaggaaatc gcgctcgcct ttttcctccc gctcgtggtc tacatggccg atgcggttgg
      781 gactcagacg gaaaccgtcc tcgtgcgggc cctggcctac ggcaaaatct ctatagccga
      841 tcagcttctt cgtgaaggca gagtcggctc aatgatcgga ggagccatgg ggttatcggc
      901 cggattgggc ctatggatcg tacagggacg accggcagtt gcgctcgtcg tggcattgac
      961 gctcgccgta acggctctcg tcgctacgat ggtcgcaagt cttcttccca tgggactcga
     1021 ccgtcttgga gtcgatcctg cgcttgcgag cgggcctgtc gccaccgtgc tccaagatat
     1081 cctcagtgtg gcgatttatc tcagcatcgg aacgacgatt cttggctgac aatcagggca
     1141 actagggtcg ggaggtgttt gatgcccatg tatgactaca aatgcttaga ctgcggaaag
     1201 gagtctttgg tggtgctgac attgaaggag catgaagccg gcaccgctaa gtgtccggcg
     1261 tgtaacagca ccaacatgaa gcagctgtac acctctttca tcgcccatac caccaagaag
     1321 agctgacatg accatgcccc tcttggcacg aataagtggt ctgatgatcg cctctcttgc
     1381 agtgcctgcc atgcttttgg ctcaggagta tcctcccgat ctctcgcgcg gcaaggcggt
     1441 gtatgaacgc cattgtctga gctgtcatgg cgcgagcggg cgtggcgacg gaccggccgc
     1501 ggcatcattg agagtcgcac cggccaactt ccaacggttt ggatccttcc tgaaatctga
     1561 cgaggaactg ttaaggacca tcgagcatgg ggttgtattc agtccgatgc attcctggcg
     1621 aggccaactc acggatgagg aaatgtctga cgtcttgtct tacgtccgct ttctctctca
     1681 gcaggcccgg tgattgcctt cgcacgcgtc cttcgatcac acatgttgtt tcctgatggt
     1741 gtcatgagtc cattcgaaaa aggaaagtca tatgacaagc gcacataggg ttctcggcca
     1801 ggcggtggtg aggctgggct tcgccgttat cctcgtcggc acggctttga cggagacctg
     1861 gccggcggac atgtccaatg cgtccggatg caccagcccc tataaaaaca aggcggttgc
     1921 tgcgaacagg ctgcgcgcca tcgtgcaatc gcatgtgcga tggctggaac aatggtatga
     1981 ggggggtcag cgtgcggatc tttgccaatc ggaccttcgc caagcctcgc tcaacggcgc
     2041 caacttggag cgcgccaacc tggaaggggc catgttacgg caggccaatc tctatcagag
     2101 cctcctgact caggccagcc tcgcgggagc ggacctcaca agcgcgggtc tcgaggacag
     2161 caacctgtcg ggagccgatg cccggaaggc gcgtttgtca cacgccaatc tctttcgagc
     2221 cataggagat gaggcggcgc tatttgacgc cacactgatt ggagctcaac tgcacgagtc
     2281 cagcttcgaa cgggctcatt tcgaaggcgc ggatctgaca tcggccgatt tcagcaatgc
     2341 gaacctcgtc gaggcgttct tctacggcgc caatctccac cgtgcgatct tgtcgggagc
     2401 tgatctcctg caagctgatc tccgccgggc gaatatgacg aaggccatcc tcaacaagac
     2461 ccatatgcag ggcgcccttc tcgaccatgc gcacctgtcg ggcgctcgcc tgatcgaggc
     2521 tgatttagaa agcgcttttc tggatgaagc cgacttgaca gaagccagcc ttcccgatgc
     2581 cttgctccga ggcgctgacc tccgcttcgc gaatcttcac gcgacgggtc tgcaacgtgc
     2641 cgatcttgag ggagtaaatt tcgaaggggc ccgtctggtc aaggccaatt tacagacggc
     2701 gaatctgaaa agggcgattc tgtatcatgc ggtgttcgac gaggctgact tgcgcgatgc
     2761 cagacttcat caggcggtct tgatcggtgc gagaggcagc ggcgccgtct ttaccaaagc
     2821 ggacctgagc gacgtgtacg ccccgagagt ggtgttgcag aacgccatct tcagcgaagc
     2881 gaagcttgaa tcggcgaatt tggttgccgc aaacttgaga cgagccaatt tgagcagtac
     2941 gaatcttgct catgccaatc tgcaggaaac caaccttcaa gaggccttat gcccaggggc
     3001 cgatttcact ggtgcgcaaa tggatggcgc cgatctccgt ttggccgact tgcgcgatgc
     3061 gaagttggcc gaagccatcg gtttgacgca ggggcaactt gatgcagcct gtttgaacga
     3121 gcgaacgaca ttaccgcctg acctgacccg gccgccccct tgtacagcgc acaataagag
     3181 gcgtcgtgac taaaagcgga ggcgcaatcc ctccttgcac gcattcccct ccgatgttcg
     3241 caggagggtt gatcctcaca ggaccgatgc cccaaacgta agttgagtgc gtttcaagct
     3301 cctccgcacc cgtcttacgg tgctgagagg ccctggccga aggttcggat ataggcaacg
     3361 acgtcccgaa tttcttgatc cgacaatgca tgtttccacg cccccatggc cgtattggct
     3421 cgcccttcgt gaatcgagtt gtagagtccg gaatcgagct tcgcctgcac ctgtcggctg
     3481 gtcaggtcgg cggccggcgg gttgaacttc atgacccctg gtccctttcc gtccagtcca
     3541 tggcacgtct tgcacaattc aaaatagata cgcttacctg ccgtcatgtc ggcattgcct
     3601 gcgtggatga acgacggctg tcctgcgaga agcacaggca gcgtgatcac ggcaaccaca
     3661 gcattcctat gaaacattct gccctccttg gcttaaatta agcttgatgt aagaccgctg
     3721 aggttcatgg ttttgccacc accgactttc gtgcaccgag tattgtcagg tcaatgactg
     3781 tgagatggct ccgctcggcc gcgcgtcctg ctgtggagac aaaggctcca tctaacctat
     3841 ccagctgttc tctccagcaa cggatccaac tatacgaaag cgaacgacgg agacgatcac
     3901 actagaaccg gtactgcccg ccgatcgaga caattgtgtc gttgaaattc ctcagcgcat
     3961 tcgtcgacgc gcgctgagta tgttgaaaac cgagcgtgac catggcgaca ggggtca
//
LOCUS       DASSCQ010000174         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1694199, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000174 DASSCQ010000000
VERSION     DASSCQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694199"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFH05_11635"
     CDS             complement(<1..479)
                     /locus_tag="VFH05_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET6678916.1"
                     /translation="MLKASLKRYFLTGLLVITPIWGTFLILKTLFVAVDGILGDLLAQ
                     LVPNHYVPGLGIVTLILLVFTTGLFAANFIGRQIVKLWEGWLNRVPLVRGIYSTLKSM
                     MDILSFSDRGSYHRVVLIQFPKNGHYCFAFVTGVSKGDMQDLAQEPLVHVYVPTSPNP
                     "
     gene            608..1282
                     /gene="tmk"
                     /locus_tag="VFH05_11640"
     CDS             608..1282
                     /gene="tmk"
                     /locus_tag="VFH05_11640"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET6678917.1"
                     /translation="MARPGTRRQARGVFLTLEGPEGSGKSTQIAFLGAALRKAGYPVV
                     YTREPGGTKLAEIIRGTLLSASSAEPIAPETEAFLVLAARSQHVTHLIRPALARGSIV
                     LCDRFADSTFAYQGFGRRLSLSWLQTANHAATRGLLPHFTLLLDVPVSVGLARRRQAS
                     GKLNRLDRESQQFHMRVRKGFLTLASRTPRRIKVINANRPAHVVRAEIEAVVLGWLGK
                     RRRRGG"
     gene            1289..2314
                     /gene="holB"
                     /locus_tag="VFH05_11645"
     CDS             1289..2314
                     /gene="holB"
                     /locus_tag="VFH05_11645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET6678918.1"
                     /translation="MPFGSIVGHHSTIASLQTAVSRQRLAHAYLFHGENSIGKRTTAV
                     SFAQALNCDSAEPTRIDGCGVCRSCQQIEARTHPDYFVIEPDRELATPQIKIEQVREI
                     EQQIVYRPLIGERKICLIDDADRLTISAANALLKTLEEPPDHSLFLLITSRPASLPVT
                     IRSRCQSLRFATPARTDVEAALILKREMPPSDARLLAMISEGRLGEALSTDLKQVRAE
                     QQELLELMSPDSLRSITTLLAAAESVAKADRATELLTWLARWIRDLVIIQVGGDRDQL
                     LHLDHMPVLERYARQAQTDTLLSVLSDIDHTLRQATRHLNLQMALENILFRLRDAIMT
                     SVPQPVS"
     gene            2376..>3046
                     /locus_tag="VFH05_11650"
     CDS             2376..>3046
                     /locus_tag="VFH05_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6678919.1"
                     /translation="MRDQHTFYITTPIYYVNDVPHIGHAYTTIAADVLARYWRLRGRD
                     VFFLTGLDEHGQKVQQAAAKAGIEPQAYCDRLAPQFDALWKRLNVTNDAFIRTTEPPH
                     KEIVQRYLQQLFDKQLIYKDSYTGWYCTFDERFWTEKDVVDSLCPDCKRPVERLSESN
                     YFFKMGQYQDSLIKYIKTHEKFIRPNSRRNEVLGFLETQKLGDLSISRPKSRLSWGIE
                     LPFDK"
BASE COUNT          692 a          871 c          813 g          670 t
ORIGIN      
        1 ggattcggcg aggtcggcac atacacgtgc accagtggtt cctgcgccaa atcctgcata
       61 tcgcccttcg acacgcctgt cacaaatgca aaacaataat gtccgttttt tggaaattga
      121 atgagcacga cgcgatggta ggacccacga tccgagaagg agaggatgtc catcatggat
      181 ttcaatgtcg agtagatgcc gcgcaccaac ggcactcgat tgagccagcc ttcccacaat
      241 ttgacgatct gccttccgat gaaattggcc gcaaacaagc cggtcgtgaa caccaggagg
      301 atcagcgtga cgatccccaa gcccggaaca taatggtttg ggaccaactg tgcgaggaga
      361 tctccaagga tgccatctac ggccacgaaa agagtcttta aaatgagaaa ggttccccag
      421 atcggggtga tgacgaggag accggtcaga aagtagcgtt ttagcgaggc tttcaacata
      481 aatagtggta gacagggatt gccgctcatc atacagatat tgctggcgcg ctcgcaaggg
      541 ttttattgct attttcaata acttggacta ctatcccgcc cgtttttcag tcgcactgga
      601 gtgagtgatg gcgagaccgg ggacgaggcg tcaggcgcgg ggggtgtttc tcacgctcga
      661 aggcccggag ggcagcggga aaagtacgca aattgctttc cttggtgctg ctcttcgcaa
      721 agccggatat ccggttgtgt atacccgcga acccgggggc acgaagctcg ctgagattat
      781 tcgaggaacc cttctttccg cttcatccgc ggaaccgata gcgcctgaaa ccgaggcatt
      841 tcttgtgctt gcggccagaa gccagcatgt cactcacctc atccggcctg ctcttgctcg
      901 aggctcgatt gtcctttgtg atcgttttgc ggattccacg ttcgcctatc aaggctttgg
      961 ccgacgtctt tcgctttcct ggttacagac tgccaatcac gcagccaccc gcggtctgct
     1021 gccgcatttc accttgctcc tcgatgttcc ggtgtcggtg ggtttggcgc gccgacggca
     1081 agcgtccggc aaactgaacc ggctggatcg agagtcccag cagtttcata tgcgcgtccg
     1141 caagggattt ctcacacttg cttcacgaac accacgccga attaaggtca tcaatgccaa
     1201 tcggccggcc catgtggttc gagcggagat agaagctgtt gtgttaggct ggctaggaaa
     1261 acgacgtcgt agagggggat gagcgcgcat gccgtttgga agtattgtcg gccaccattc
     1321 gacgatcgca tccctgcaaa ctgctgtgag ccgccagcgt ctcgcccatg cctacctctt
     1381 tcacggcgaa aattcaatcg gtaagcgaac gacggccgtg tctttcgcac aggccctcaa
     1441 ttgcgacagc gccgagccaa cccggatcga tggctgcggc gtgtgccggt catgccaaca
     1501 gattgaggcc cggacgcatc cggactactt tgttattgaa ccggaccggg aactggcaac
     1561 tccgcagatc aagatcgaac aggtccgaga gatcgaacaa cagatcgtgt atcggcctct
     1621 catcggcgaa cggaagatct gcctgatcga tgacgccgac cgcttaacca tcagtgcggc
     1681 caacgcgctg ctcaaaacac tcgaagaacc accggatcac agcctgtttc tcctgatcac
     1741 cagccggccc gcctcgttgc ccgtgaccat tcgctcgcgc tgccagtctc tccgctttgc
     1801 gacgccggca cggactgatg tggaggcggc tctcattctc aagcgggaga tgccgccatc
     1861 agatgcccga ttgcttgcaa tgatcagcga agggcgcctc ggagaggcct tgtcgaccga
     1921 cttgaaacag gttcgtgccg agcaacagga actgcttgag ctcatgagtc cggattccct
     1981 ccggtcaatc accacgctgc tggccgcggc agagtccgtc gcaaaagccg accgagcgac
     2041 ggagctcctt acatggcttg cccgctggat ccgagatctc gtgatcatcc aagtaggcgg
     2101 cgatcgcgat caactgctcc acctcgatca catgcctgtg ttggaacggt atgccaggca
     2161 ggcgcaaacc gacaccctgc tttccgtgct gagcgacatc gatcacaccc tacgacaggc
     2221 gaccaggcac ctgaatttgc agatggcgct tgagaacatt ctgtttcgcc tgcgcgatgc
     2281 gatcatgacg tcggttcccc agccagtttc gtgagacgag tcaataccag gcgttggttt
     2341 tcgtcctctt cgactgttaa ggttgaaatc tgaccatgcg cgatcagcat accttttaca
     2401 tcaccacgcc aatttattac gtcaatgacg tcccgcacat cggccatgcc tacacgacga
     2461 tcgccgcaga tgtgctcgct cggtactggc gacttcgagg gcgcgacgtc ttttttctta
     2521 ctggccttga cgaacatggt cagaaagtcc agcaagccgc cgccaaggcc ggcatcgagc
     2581 cccaagccta ttgcgacagg ctggcacctc aatttgacgc gctctggaaa cgcctgaatg
     2641 tgaccaatga tgcctttatc agaacgacgg aacctccaca caaagaaatc gttcaacgat
     2701 atcttcagca gttgttcgac aagcaactga tatataaaga ttcatataca gggtggtatt
     2761 gcactttcga tgaacggttc tggactgaaa aggacgtcgt cgacagtctc tgccccgact
     2821 gtaagcgccc tgtcgaacgg ctcagcgagt ccaattattt ctttaagatg ggccagtacc
     2881 aggacagtct gatcaaatac attaaaacgc acgaaaagtt tatccgtcct aactcgcgac
     2941 gtaatgaggt actgggattt ctcgagacac agaagctcgg tgatctctct atttcccgtc
     3001 cgaagtccag actttcctgg ggcatcgagt tgcccttcga taagga
//
LOCUS       DASSCQ010000175        16256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1522832, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000175 DASSCQ010000000
VERSION     DASSCQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 16256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16256
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522832"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFH05_11655"
     CDS             <1..940
                     /locus_tag="VFH05_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET6678920.1"
                     /translation="VRHGFLGLANNWIEAVTEEDTRGMSSHASSPIGSSRFEDFKEEA
                     VQQAAMRNLKPYLDDAAVVVLGGDGSLRGARAVYEGFGVQVVGIPGTIDNNIEGTVSL
                     GYHSAIALADQSIESLKATSAAMGSIFFVEVMGAGSGHLALACAYQARAEGLLVNEHP
                     DPNAYIDDIILGTLKRTLGVPNKSHLFVVAERTPHRHHSEGGVHGLVDYVASVIAQWP
                     QRQVQSNHYPMTLATKATILGHTLRGAPPTPEDKAIGQHLAFETVQRLVERPEDVVGS
                     MVAYRERGVIENIPLHAVTAKQFDWDLFARMHGSES"
     gene            complement(1015..1986)
                     /locus_tag="VFH05_11660"
     CDS             complement(1015..1986)
                     /locus_tag="VFH05_11660"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6678921.1"
                     /translation="MEGDEKTLIDCHVHLAALPEGDNGCYISPKMLKSPLFRFLLWKH
                     GLSPDKPREANRKYVDDLLVELRASRQVQRAVLLGMDGVYDHAGELARKETDFLISNE
                     YVLQTARAHPDEFLPGVSINPQRRDAVEEVHRCADAGAALVKVLPNAQQFNPADGKYT
                     AFYRALAERNLPFLSHVGYEFSLIGKDQSVGDPGRLRLALDEGVTVIAAHACSYGLML
                     YERFLPVLLTFAKQYPNFYADISALTLPNRFRMLLHLRRHPEVHHRLLFGTDYPLSVM
                     HVAAWGRVGLRSLARMMQTTNRFDRQALVCRSLRVNFSSFDRLLSRR"
     gene            complement(2056..5460)
                     /locus_tag="VFH05_11665"
     CDS             complement(2056..5460)
                     /locus_tag="VFH05_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HET6678922.1"
                     /translation="MTHPLRGLLIAQFCGAFNDNAWKLMVALLGIRQLASTMAPGPEF
                     ETASQTQTTLAFVIFTFPLAVVSMVAGLLADRVSKRTVIILMKAVEILLMSAATLALW
                     MNPAGGFLPLIVLAGMGVHSALFSPAKYGILAELLPHARLAAGNGQLEMWTFLAILTG
                     TAAPGILLSVTGTAVWMAPLLLTALALIGFSAAWTIPRVPPARSEGGLANTLQGAWLA
                     LARDRMLRLAICGAVFFWTIASLFAQNILVYAKAVLGVSDWQSGLPLMMLSIGIGIGA
                     RWVGRLSQGRVEYGLIPLGAAGVAVMLLTLGLLAPGLPATLALMVILGISSALIFVPL
                     NALIQWRAPDDRRGSVIAFENTCVFTGILLGSLGAGALASGGTSTTGIFTAAAAFTVL
                     GTAWAMRLLPEALLRVVLVIVTNSLYRLRIVGHAHVPTTGGALLVPNHISFVDGLLLI
                     ASLDRPVRFVVDAQYAEQPFFKPIMKVFGVIPIAPYGGLRVVLRALREAGAAIDRGEL
                     VCIFPEGQITRTGTLLPFRRGFERIMKGRTAPVIPIHLDRVWGSIFSFDRGRFFWKIP
                     ERLPYPVTVSFGKPLPPQATAHEVRSAIQALGEAAWALRKPDRRPLHREFVHAMRRRP
                     FQFVMSDQSRPHVSAMQALIGSIALARALRTSWENQHHVGILLPPSVAGALVNVAAPL
                     CGKTSVNLNYTVGKSGLDASVRLAGLRTIVTSRTFVEKAKLELPDAPAIVWLEDLAQT
                     IRRKEKFLAAMLACMAPVRLIERVCGQRIPLSIDAPATIIFSSGSTGEPKGVVLSHYS
                     VDANAQGATQVLHLYRHERVLGILPFFHSFGYLVFWFVMFNDAGLVFHPSPIDAAGIG
                     ELVRRQRVTFLVTTPTFLQLYARRCSPEQFSSLRVALTGAEKLSLRVSQAFKDRFGIE
                     PIEGYGVTECAPVIAVNCPDFRAAGYYQPASRRGTVGQPLPGISVSIVDPDSFTPLPL
                     GTPGMLLVKGPNVMTGYLGRDDLTASVMRDGWYITGDIATLDDDGFLTITDRLSRFSK
                     VGGEMIPHGRVEEALHQAAESEVPVFAVTGIPDERKGEQLAVLHTLDESRIPDILARL
                     SSNGLPNLFIPARANFVKVESLPLLGTGKMDLRALKRIAVERLTGR"
     gene            complement(5470..7401)
                     /locus_tag="VFH05_11670"
     CDS             complement(5470..7401)
                     /locus_tag="VFH05_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678923.1"
                     /translation="MDLLNESCREHEAGCSTAWFIIDAMVSFQYRHRTMAVRVRARQL
                     QQLIGRIERLIARGTAASSRFTTYRLAIFIAGTLTSITLFRSGQYHTGNIALAAFVIL
                     FLIVASYHNRLEQRIHRLRLWALIKSAHLARVQLAWSQVPHKTSESLSSHLYAHDLDL
                     VGPHSLLHLIDTTVSDYGRTRLTSWFLTQPPDPKVWDTRQRLVKELAPRSLFRDRLVL
                     EATLAGEQEINGRRLAAVLEHPVGFPGLPRTLILQAMLAAGTIVLGVGALLDWLPGYW
                     MLTFGMYALIYLMTDQGEELLEHAVGLHHEMERLSRVLRFLERHAARPDSAMAQTCAP
                     LLEAGRSPSAFTRHIARIFHGISVKAHPLIHLGLNALVPWDLFFAFRLRQFQERVRTR
                     LPLWLDRLAEVEAASALATFAYLHPDYAWPTPAPAVGLASHNLGHPLIPSTTRVGNDI
                     ALEKLGTILFITGSNMSGKSTFLRTVGINLCLAQAGAPVCASTFTWTWSRLGCCIRVN
                     DSLDAGLSFFYAEVKRLKTILHATTDRASPPVLFLIDEIFKGTNNRERLIGSRSYIKA
                     LATGNGYGLVSTHDLELTDLEKEIPSLVNAHFQETVSDGALSFDYQLRPGPCPTTNAL
                     RIMELEGLPIPRTSSHEAQ"
     gene            7402..8259
                     /locus_tag="VFH05_11675"
     CDS             7402..8259
                     /locus_tag="VFH05_11675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET6678924.1"
                     /translation="MASDYIEVQISSRVDAGEVMSLLGDSSVQGAWEDERSIHLYWPT
                     TEWSPDRLAQLRRTLRHLIGPGLPEADIVVRTMQNQDWNRQWAESVKPLWIGRRLVVR
                     PSWESVAIGSDQIEIILDPKQAFGTGHHATTRMLLEWLEHVIQGGETVLDVGTGSGLL
                     AMVALRLGADTAVGIDHDADAIDCAREYASVNGFGPELSLQCGILAGDRPYDLVLANL
                     DRQTLMMLAAPLEASTGRMLLVSGLLRDQCAEIVEAFARVDLFPCSVREQDGWGAIEF
                     RRAQSCEGA"
     gene            8262..8774
                     /locus_tag="VFH05_11680"
     CDS             8262..8774
                     /locus_tag="VFH05_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET6678925.1"
                     /translation="MGPQGRPPYPHVHQINVSDGGVPKYPVFEAKIDKEGLDGDRQRN
                     LKVHGGPDRALCLFSLELIERLQDEGHSIDSGYSGENLTLAGLEWDHIEPGVQLRIGP
                     EVCLEMTSYCAPCEWNAQWFRDRDISRISQKANPGWSRLYARVLQGGVVRPGDKVEIS
                     NRPSASERPR"
     gene            8771..9433
                     /locus_tag="VFH05_11685"
     CDS             8771..9433
                     /locus_tag="VFH05_11685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6678926.1"
                     /translation="MMERTPEPELMDDPDQTKAYAAADFAEENQGFVDRFGEYFPDFS
                     GGRVLDVGCGPADIPIRFARRYATCHVLGVDASEPMIRLGQEAVQRAGLGERITLRCE
                     RFEAIAGANVFDAALSNSLLHHLPNPLQFWHKLRQLVKPGAPVLVMDLLRPDSREEAQ
                     AIVDRYAATEPDILRRDFYNSLMAAYTEDEVTSQLARMNLTRLLIDVIDDRHWVVGGT
                     IY"
     gene            9396..10304
                     /locus_tag="VFH05_11690"
     CDS             9396..10304
                     /locus_tag="VFH05_11690"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET6678927.1"
                     /translation="MTAIGWLVEPSTERMVERVEATHYLRRALRRMYPILTIALVMLF
                     VCDATVSRAQLDRLRKSKSMMANPIPDTPMVEIPAGPFVMGSDGSQALEDERPAHRVW
                     LDGFSIDAEEVTTAQYAAFLAATTRAAPWQWETVDLMQHADRPVIGVDWHDADAFCRW
                     KGKRLPTEAEWEKAARGIDGRLYPWGNQVPTPDLANFALGARFSYNQVLLPVRSHEQG
                     KSPYGLYHMAGNAYEWVQDWYGTNYYEMSPDRNPQGPAQGQFKVLRGGSWSDLPKYLL
                     TYGRFKLLPDTKNSYTGFRCAMSEKK"
     gene            complement(10345..10575)
                     /locus_tag="VFH05_11695"
     CDS             complement(10345..10575)
                     /locus_tag="VFH05_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678928.1"
                     /translation="MSWTSNSEADLAGYKLYVGTAPGRYTYAGSPVIIGLMGSYTISG
                     LPIGQTYYFALSAFDSSGNESALSSEVSKSIY"
     gene            10577..10720
                     /locus_tag="VFH05_11700"
     CDS             10577..10720
                     /locus_tag="VFH05_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678929.1"
                     /translation="MALPVPDDVDAGGCDKVVDGSDEGGAEDCRGADGGGGEEIVTVG
                     AII"
     gene            10805..11164
                     /locus_tag="VFH05_11705"
     CDS             10805..11164
                     /locus_tag="VFH05_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678930.1"
                     /translation="MLARPPTAVLVMGAALVSGTVVEVLEAVAGALGTREPADARPVA
                     GSEARLSLPVFCMSVVTDSVVAAVVCGVAATAPRIVVCAGRFASFRGSGRGAGVDEAA
                     AVGGKLVAGSSGRRSDA"
     gene            complement(11475..11924)
                     /locus_tag="VFH05_11710"
     CDS             complement(11475..11924)
                     /locus_tag="VFH05_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678931.1"
                     /translation="MITEGVQQTLTVLYPLYKNEVYQRREHMIRLTAFGAFGLIAMIF
                     ALLLSPVKSRLSSPESVLLALGSLTWCALFCGLVLQQQHRHRLAKQVLIQLEQALGFY
                     EDGLFVDHQTLYPAKWKTAWLTDRTGLHYYASLCGLTGIFLFALILV"
     gene            complement(11964..12728)
                     /locus_tag="VFH05_11715"
     CDS             complement(11964..12728)
                     /locus_tag="VFH05_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6678932.1"
                     /translation="MEESLTFHDERGHRISAILSRPAQPTTAVCVLCHGFLSGKNSTT
                     NKTLTRLLNEKAIATFRFDFFGQGESDGHFEDITPSLAVGQTHAAVTLVHARGFGKIG
                     LVGSSFGGLVSILTAAQRPDIACLALKCPVVDFAEELRLEFGPDELTRWQVTDTIPNL
                     MGGPERIRLKYGLYEDSLQQIAYGPAAQIVAPTLIVQGACDELVPLHQSRRLLDALRG
                     PKRLDLLPQADHQFTRGEDFRTMTTAMTDWLVRYLV"
     gene            complement(12886..14220)
                     /locus_tag="VFH05_11720"
     CDS             complement(12886..14220)
                     /locus_tag="VFH05_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678933.1"
                     /translation="MKRSLLSSQSEGLERRPTSRLVRPSMSGTPLTFLLTGFMWLSVS
                     FLLGAALLLGLVNGTPLPHWLKPVHVHGALLGGFLQMIIGGFLFSRANGHTHASATSH
                     SSLFWALNGATVGLLVSFWLGHMTMAGLAALVLIGIVLWISPKAWLRIGNAYQPAGAV
                     WIARAAMVALLLGLIAAALMTFRIIEGAQSYLRLFHVHFLVLGFLTVTFILALHQILP
                     VLMQAPLASHALTRIALWFLPVGFAALLGGFVTSSLWLEITVGGLLVAAVAMCTYDFL
                     VGWIKSGAPEHAASDHLLLGVCFLFLSTVAGLAMGANYLRTPPVMPIGSLHLVAYTHL
                     AFIGFMTQVICGSLATGVPDILAVTRVPNSKKRQAYRAQLDGIMNRWRTAQLAGMSLG
                     TMALAVLAALTWSVPLGSSSVQITVWIAAGLLILSLTLFAVKLTWAVGFRPS"
     gene            complement(14217..15020)
                     /gene="thiD"
                     /locus_tag="VFH05_11725"
     CDS             complement(14217..15020)
                     /gene="thiD"
                     /locus_tag="VFH05_11725"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET6678934.1"
                     /translation="MSRSLKQVLTIAGSDSGGGAGIQADIKAMSANGVFAMSVITAIT
                     AQNTEEVTEVFDLPCSIIAAQIDAVFDDFDVAAVKTGMLSSDSIVETVAKMLKPQEVA
                     NLVVDPVMISKSGHSLLKPDAIEAMKKQLFPLAILVTPNIHEAQQLSGIQITSLADAR
                     RAAKVIHGLGSKHVLIKGGHLLAERATDLLYDGRFFNVFKGEFIDTPHTHGTGCTYAS
                     AIAAQLARGKTITDAVAAAKTYVTEAIRHSLAIGHGAGPTNHFYFLPES"
     gene            complement(15079..15567)
                     /locus_tag="VFH05_11730"
     CDS             complement(15079..15567)
                     /locus_tag="VFH05_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678935.1"
                     /translation="MSSTATDSPRRFTVLTLVLYWSCTLSGLAIPWIATVAVDMAKHD
                     QSLAGAVRQLRLHLFAPGYNLFVIALINAMPFMIFAVFLLFHLGRAPMDDRTLRGRRS
                     AGVLMTVIGLVAFSLWTHLTTLWQADAQAALAYLFSPFLLLLLIPICYAFGRALSTLV
                     FR"
     gene            complement(15564..>16256)
                     /locus_tag="VFH05_11735"
     CDS             complement(15564..>16256)
                     /locus_tag="VFH05_11735"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6678936.1"
                     /translation="AARKVMVRWLPKLQDPDPQVRRDAAAALGALGPVAKPAVHSLLG
                     IANDPNTIVRDRVVRALAAIALPPELVARGLTQALQDPEWTVRYAAISPFSFGGVLNP
                     ESLAALRPLVHDPNEMVARLAQSAVAAADHPIPVSVHIFSLDQGSDRTLPLLQLTKLG
                     PRAAEAVPKLASLLTAERPLERYLAACTLQSVGPSAVQAVPALQRTLDDPDPIVREAA
                     AEALQAIEARRP"
BASE COUNT         3479 a         4575 c         5162 g         3040 t
ORIGIN      
        1 cgtccggcac ggcttcctgg gactcgcgaa caactggatc gaagcggtga ctgaggaaga
       61 cacccggggg atgagcagtc atgcgagcag cccgatcggg agcagccggt tcgaagattt
      121 caaagaagag gccgtgcagc aggcggcgat gcggaatctc aaaccgtatt tggacgatgc
      181 ggcggtggtc gtcctgggcg gcgacggaag tctccggggt gcacgagccg tgtatgaagg
      241 atttggtgtc caggtcgtcg ggatccccgg cacgatcgac aacaatattg aaggaacggt
      301 ctcgcttggc taccactcgg ctatcgccct cgcagaccaa tccatcgagt cgctgaaggc
      361 gacgagcgcg gcgatgggaa gtattttctt cgtcgaagtc atgggggccg gttcgggaca
      421 tttggccttg gcctgcgcgt accaggcgcg ggcggaagga ttattggtga acgagcatcc
      481 ggacccaaac gcgtatatcg acgatattat cctcgggaca ttgaaacgga cgttaggagt
      541 gccgaacaaa agccatttgt tcgtcgtcgc cgaacgtacg cctcatcgcc atcattcgga
      601 gggcggcgtg cacggcctcg tggattatgt ggccagtgtg attgcgcaat ggccacagcg
      661 gcaggtccag tcgaaccact atcccatgac actcgcgacc aaggcgacta ttctaggaca
      721 cacactgaga ggcgctcctc cgacgccgga agacaaagcc atcggccagc atttggcgtt
      781 tgagaccgtc caacgcctgg tcgagcgccc tgaggatgtg gtgggcagta tggtggcgta
      841 tcgggaacga ggggtcattg aaaacatccc gctgcatgcc gtcacggcaa aacagttcga
      901 ttgggacttg tttgccagga tgcacggcag cgagtcgtga ggaaaggggt cagcgaagag
      961 cgttccggtt gctgcccgag ccgaccgcga gacgcccgtt gtctcccatt gctgctatcg
     1021 tcgactgagt agtcggtcaa acgacgagaa gttgacccga agcgagcggc acacgagcgc
     1081 ttggcgatcg aagcggttag tcgtttgcat cattctggcg agggagcgga ggccaacacg
     1141 cccccatgcg gcgacatgca tcactgacaa gggatagtct gtgccgaaaa gcaaccggtg
     1201 gtgaacttca ggatgccggc gcaagtggag cagcatacgg aagcggttcg gaagcgtgag
     1261 cgcggaaata tcagcgtaga aattcggata ctgtttcgca aaagtcagca gcaccggaag
     1321 aaaccgctcg tacaacatca atccataact gcaggcatgc gccgcgatca ccgtcacgcc
     1381 ttcgtccagt gccaaccgga gccggcccgg gtccccgaca gactggtcct tgccgatcag
     1441 gctgaattcg tatcccacgt ggctcagaaa cggcaggttc cgttcggcga gcgcccgata
     1501 gaaggcagtg tatttgccat cggcaggatt gaattgttgc gcgttcggca ggaccttgac
     1561 taacgccgcg ccggcatccg cacagcggtg gacttcttcg accgcatccc gccgctgagg
     1621 attgatggac acgcctggaa ggaactcatc tggatgggcg cgagcggttt gtaagacgta
     1681 ctcattgctg atgaggaaat cggtctcctt gcgggcgagc tcccccgcat ggtcatacac
     1741 gccgtccatc ccaagcagga ccgctcgttg cacctgtcgg gaggcgcgca attcgacaag
     1801 caaatcatcc acgtatttgc ggttggcttc ccgtggcttg tccggcgaga gcccatgttt
     1861 ccacagtaaa aaccggaaca gcgggctctt gagcatcttc ggcgagatat agcagccgtt
     1921 atcgccctcc ggcagcgcag ccagatggac gtggcagtcg atcagcgtct tttcatcacc
     1981 ttccatcgtt gcgtttgagt gctcactttc tgttgaatgt ctggtctcgt gtgtaaggtt
     2041 tggatttcca aaaccttatc gccctgtcag ccgttccacg gcgatgcgct tgagcgcgcg
     2101 cagatccatc ttgccagtgc ccagcagcgg gagactctcc actttaacga agtttgcccg
     2161 tgccgggata aagagatttg gcaagccgtt agaagacagg cgcgcgagaa tgtccgggat
     2221 gcgtgattcg tcgagcgtgt gcaggacggc gagctgctcc cccttgcgtt catcgggaat
     2281 accggtcacg gcaaacaccg gcacctcgct ctctgcggcc tggtggaggg cctcttcgac
     2341 cctgccatgc ggaatcattt ctccgccgac cttcgaaaag cgtgacagcc ggtcggtgat
     2401 cgtcaaaaac ccgtcgtcgt cgagcgtggc gatgtcgccg gtgatgtacc atccgtcccg
     2461 catgacgctt gccgtgagat cgtcccgccc gagatagccg gtcatcacgt tgggcccctt
     2521 gaccaacagc atgccgggag ttccaagagg cagcggggta aaactgtccg gatcgacgat
     2581 gctgacggag atgccaggca gcggctgacc gactgttcct cggcgcgagg ccggttgata
     2641 gtagcctgcg gcgcgaaagt ccggacagtt gaccgcgatg accggcgcac attccgtcac
     2701 gccgtagccc tcgatcggtt cgatgccgaa gcggtccttg aatgcctggg agacgcgaag
     2761 ggacagcttc tccgcacccg tcagggcgac gcgcaaagaa ctgaattgct cgggactaca
     2821 ccgccgggcg tagagctgca aaaaagtggg cgtggtcacc agaaacgtca cccgctgccg
     2881 gcggaccagt tccccgatcc cggccgcatc gatcggagag ggatgaaaga ccagtccggc
     2941 gtcgttgaac atcacgaacc agaacaccag atacccgaac gaatgaaaga acggcaggat
     3001 tcccaggact cgttcatgtc gataaagatg gagaacctgc gtcgcgccct gtgcgttggc
     3061 atccacgctg taatgcgaca gcaccacgcc tttcggctcg cccgtactgc cgctgctgaa
     3121 gataatggtg gccggagcgt cgatggaaag gggaatgcgt tggccgcaga cccgctcgat
     3181 caaccgaacc ggcgccatgc acgcgagcat ggctgcgagg aacttctcct ttcgacggat
     3241 cgtctgagcg aggtcttcca gccacacgat tgctggtgca tcgggcaatt cgagtttggc
     3301 tttttcgacg aaggtccggc tggtgacgat ggtgcgaagg ccggcaaggc ggacggaggc
     3361 gtcgagacca gacttgccca ccgtgtaatt gagattgacg ctggtctttc cgcacagagg
     3421 cgccgcgaca ttgaccagtg cgccggcgac gctcggtgga aggagaatgc cgacatgatg
     3481 ctggttctcc catgacgtcc gcaacgctcg ggccagggca atggatccga tcaaggcctg
     3541 catggctgac acatgcggcc ggctctgatc cgacatcacg aactggaagg ggcgtcttct
     3601 catcgcgtga acgaattcgc gatgcagagg gcgtcgatcc ggtttgcgca gcgcccaagc
     3661 ggcttctccc agcgcctgaa tcgcgctgcg aacctcgtgg gccgtcgcct gtggaggcag
     3721 gggcttacca aaggacaccg tgacgggata gggcaggcgt tccgggattt tccagaagaa
     3781 ccggccccga tcgaaactga agatgctgcc ccagacgcgg tccagatgga tcggaatgac
     3841 gggcgcggtt ctgcccttca tgatccgttc gaagccccgt cggaacggca acagcgtgcc
     3901 cgttctcgta atttgtccct cggggaagat gcagaccagc tctccccggt cgatggcggc
     3961 accggcttct cgcaacgcgc gcagcacgac gcggagcccg ccgtagggag cgatggggat
     4021 gacgccgaat accttcatga tcggtttgaa aaacggctgt tcggcatatt gtgcgtcgac
     4081 gacaaagcgc acaggtcgat cgaggctggc gatcaggagc aggccgtcca cgaaggagat
     4141 atgattgggc accagcagcg caccacccgt ggtcggtaca tgagcatggc cgacgatgcg
     4201 gagccgatac aggctgttgg taacaatgac gaggacgacc cgcagcaacg cttcgggcag
     4261 cagccgcatc gcccacgctg tgccaaggac agtgaacgcg gccgcagccg tgaaaattcc
     4321 tgtcgttgac gtgccgccgc tggcaagcgc ccctgcgcca agcgatccca ggagtatgcc
     4381 ggtaaacaca catgtgttct cgaaagcgat gaccgatccg cgccggtcat ccggcgcgcg
     4441 ccattgaatc agggcgttca gtgggacgaa gatcaatgcg ctcgagatgc cgaggatcac
     4501 catcagcgcc agcgtggccg gcagaccagg agcaagaagg cctagtgtta agagcatgac
     4561 cgccacgccg gctgccccca agggaatcag gccgtactcg acccgtccct gggagagccg
     4621 tccgacccat cgggctccga tgccgatgcc gatcgacaac atcatgagcg gcagtccgga
     4681 ctgccaatcc gagacgccca gcacggcctt ggcatagacc aggatgttct gggcaaagag
     4741 actcgcgatt gtccagaaga agacggcgcc gcatatggct aaccgcaaca tgcggtcacg
     4801 cgcgagcgcc aaccaggccc cctgcaatgt attggcgagc ccgccttcgc ttctcgccgg
     4861 cggcacgcgt ggaatggtcc aggccgcgct gaatccaatg agtgccaaag ccgtgagcaa
     4921 cagtggggcc atccagacgg cggttcccgt caccgacagc agaatgccgg gtgccgcagt
     4981 gccggtcaaa atggcgagga acgtccacat ctcgagctgt ccgttgccgg ccgccagtcg
     5041 tgcgtgcggc aagagttcag caagaatccc gtatttggcc ggactgaaga gggcgctgtg
     5101 cacgcccatg cctgcgagca cgatcagcgg caggaatcct ccggccggat tcatccataa
     5161 ggcgagcgtg gcggcactca tcaagaggat ctcgaccgcc ttcatcagga tgatcacggt
     5221 ccgcttgctg acccggtcgg ccagcaagcc agccaccatc gacacgacgg caagcggaaa
     5281 ggtgaagatg acaaaggcta atgtggtttg ggtttgcgag gccgtttcga actcagggcc
     5341 tggcgccatc gtggaagcca gctgacggat gcccagtagc gcaaccatca gcttccacgc
     5401 attgtcgtta aacgcgccgc agaactgggc gatgagcaat ccgcgcagag gatgagtcat
     5461 gcgtgagcgc tattgtgcct catgtgagga ggttctggga ataggcagcc cttccaattc
     5521 catgatccgc agcgcgttcg tcgtcgggca agggccggga cgaagctggt agtcgaagga
     5581 cagtgcaccg tcagagacgg tttcctgaaa gtgcgcgttc acgagtgaag ggatctcctt
     5641 ctcgagatcc gtgagttcca gatcgtgcgt gctgaccagc ccatagccgt tgccggttgc
     5701 cagggcctta atgtaggagc ggctgccaat caggcgctcg cggttgtttg ttcccttgaa
     5761 aatctcgtcg atgagaaaca ggaccggtgg cgaggcccga tcggtggtgg cgtgcaggat
     5821 ggtcttcagg cgtttgacct ccgcatagaa gaacgacaga ccggcatcga gcgagtcgtt
     5881 cacgcggatg cagcacccca ggcggctcca ggtccaggta aaggttgagg cgcagaccgg
     5941 agctccggct tgcgctaagc agaggttgat gccgacggtt cgaagaaagg tgcttttgcc
     6001 cgacatgttc gaacccgtaa taaacagaat cgttccgagc ttttccaagg caatgtcatt
     6061 gccgacgcgc gtcgtgctgg ggatcaacgg gtggcctaag ttgtggctgg cgagacccac
     6121 ggccggggcc ggggtcggcc aggcatagtc gggatgcaga taggcaaagg tggcgagagc
     6181 ggaggccgct tccacttcag ccaaccgatc gagccacaaa ggaagccgtg tccgcacacg
     6241 ttcttgaaac tgtctcaggc gaaacgcgaa gaacaggtcc cacgggacga gcgcattaag
     6301 cccaagatgg atcaggggat gcgccttcac gctgattcca tggaagatgc gggcaatatg
     6361 ccgtgtgaag gcggagggtg aacgacccgc ctcgagtaga ggggcgcagg tctgtgccat
     6421 ggccgaatca ggccgcgcgg cgtgtcgttc gaggaagcgc aggactcggc tcagccgttc
     6481 catctcgtgg tgcagcccga cggcgtgttc gagtagctct tcaccctggt cggtcatgag
     6541 atagatcaag gcgtacatgc cgaacgtcaa catccaataa ccaggcagcc aatctaacaa
     6601 cgccccgact ccaagcacga tcgtgcctgc cgccaacatc gcctggagaa tgagggtgcg
     6661 cggcaatccg ggaaagccga ccggatgctc gagcaccgcc gccaatcggc gaccgttgat
     6721 ttcctgttca cccgccaggg ttgcttcaag gaccaaccgg tcacggaaca gcgaacgtgg
     6781 agcgagttct ttgacgaggc gctggcgggt gtcccatacc ttcggatcag gtggttgggt
     6841 caggaaccat gatgtgaggc gggttcgtcc gtaatccgac acggtggtat cgatcagatg
     6901 cagcagtgag tgtggtccca ccaaatccaa gtcgtgggca tagaggtgtg acgacagcga
     6961 ttcagaagtt ttgtgaggaa cctgcgacca ggcaagctgg acacgcgcga gatgggcgga
     7021 cttaatcaac gcccacaggc gcagccgatg aatccgctgt tcaagcctgt tatgatacga
     7081 cgcgacaatg aggaagagga tgacgaatgc ggccagggcg atgttgccgg tgtggtactg
     7141 gcctgatcgg aacagcgtga tgctggtgag cgttcccgcg atgaagatgg ccagacggta
     7201 ggtggtgaat cgggagctgg cggcggtccc gcgcgcgatc aaccgttcga ttctgccgat
     7261 cagctgctga agttgccggg cgcgcacgcg taccgccatt gtccggtgac gatactggaa
     7321 gctcaccatg gcgtcaataa tgaaccaggc cgttgaacaa ccggcttcgt gttcacggca
     7381 gctttcattg agaaggtcca tatggcatct gactatatag aggttcagat ctcctctcgc
     7441 gtggatgccg gcgaagtgat gagcctgctt ggggattcgt ctgttcaagg cgcctgggag
     7501 gatgagcgca gtattcatct ctattggccg accaccgaat ggtcgccgga ccgtttggca
     7561 caactcaggc gcacccttcg tcacctgatc gggcccggct tgcctgaagc ggatatcgtt
     7621 gtgcgcacga tgcagaatca ggattggaac cggcaatggg ctgagtcggt caagccgttg
     7681 tggatcggca ggaggctcgt cgtcagacca agctgggagt ctgtggcgat cggttcggat
     7741 cagattgaga ttattctcga tcccaagcag gcgttcggca ccggccacca tgccacgacc
     7801 cgcatgttgc tggaatggct cgaacatgtg attcaaggag gcgagacggt gctcgatgtc
     7861 ggcaccggca gcggtctctt agccatggtt gcgctccgac tcggagccga tacggccgtg
     7921 ggaattgacc atgacgcaga cgcgattgac tgtgcacgcg aatatgcctc cgtcaatggc
     7981 ttcgggccgg aattgtctct gcaatgcggg atccttgcgg gcgatcgacc ctatgacctg
     8041 gtcctggcca atctcgatcg gcagacccta atgatgttgg ccgcaccgct cgaagccagt
     8101 accggcagaa tgcttctcgt ctctggcctg ttacgtgacc agtgtgctga aatcgtggaa
     8161 gcctttgccc gggtcgatct cttcccatgc tctgtacgag agcaagacgg gtggggggcg
     8221 atcgaattcc gacgagccca atcgtgtgaa ggagcgtgaa gatgggtcca caaggtcggc
     8281 cgccataccc ccacgtccat cagatcaatg tttcggatgg aggagttccc aagtatccgg
     8341 tctttgaggc caagatcgac aaggagggac tggacgggga tcgtcaacgc aacctgaaag
     8401 ttcacggcgg gccggaccgc gcactgtgtc tgttttcttt ggagctgatt gagcggttgc
     8461 aggacgaagg ccactcaatc gactcggggt attccggtga aaatctgacg ttggccggcc
     8521 tcgagtggga tcatatagaa ccaggggtac aactccgaat cggtccggaa gtgtgtctcg
     8581 aaatgaccag ttactgcgcg ccctgtgaat ggaatgccca atggtttcgt gaccgcgaca
     8641 tcagccggat atctcagaag gccaatccag gatggagtcg tctgtatgcc agggtcctgc
     8701 aaggcggagt cgtgcggcct ggcgataagg tcgaaatctc caacaggccg tcggcttccg
     8761 agagaccacg atgatggagc gcacaccgga accggagttg atggatgatc ccgatcagac
     8821 gaaagcctat gccgccgccg atttcgccga ggagaatcaa ggtttcgtcg accggttcgg
     8881 cgagtatttt cccgattttt caggcgggcg cgtgctggac gtgggctgtg gtccggccga
     8941 cattcccata cgatttgccc gacgctatgc gacctgccat gtcctcggcg tggatgcctc
     9001 ggagccgatg atccggttag gccaagaggc cgtgcaacgg gccgggcttg gcgagcgcat
     9061 tacgctgcgc tgcgagcggt tcgaggccat cgccggagcg aacgtgttcg atgcagcact
     9121 ctccaacagt ctcttgcatc atctgcccaa cccgctgcag ttttggcaca agctccgtca
     9181 actcgtgaag cccggcgcgc ctgtactcgt catggatctt ctccggccgg actcacgtga
     9241 ggaagcacaa gcgatcgtgg atcgatacgc agcgacggag ccggacattt tgcgccggga
     9301 cttttacaat tcgctgatgg ccgcctacac cgaagacgag gtgacgtccc agcttgcccg
     9361 gatgaacctg acgcgcttgc tgatcgatgt gattgatgac cgccattggg tggttggtgg
     9421 aaccatctac tgaaaggatg gtggagcgcg ttgaggcgac gcattacctg aggcgcgctc
     9481 tccgtcggat gtatcccatc ttgaccatcg cgttggtcat gcttttcgtt tgtgacgcaa
     9541 cggtcagccg ggcgcagctc gaccggctca gaaaatccaa atcaatgatg gcgaatccga
     9601 tacctgacac gccgatggtt gaaattccgg ccggtccatt cgtgatgggg tcggacggca
     9661 gccaggcact cgaagatgag cgcccggctc atcgcgtctg gctcgacggc ttttccattg
     9721 atgcggagga agtgacgacc gctcagtacg cagcatttct ggcggcgacc acacgtgccg
     9781 cgccctggca gtgggagacg gtggatctga tgcagcacgc cgaccgcccg gtgatcggag
     9841 tggattggca tgatgcggac gccttctgcc gctggaaggg caaacgactt ccaaccgagg
     9901 ccgaatggga aaaagccgcc agaggaatag atggtcggtt atatccttgg ggcaaccagg
     9961 tcccgacccc ggacctggca aattttgcgc tgggcgcgcg ctttagttac aaccaagtcc
    10021 tgttgccggt ccgttcgcac gagcagggca agagccctta tgggctctac cacatggcgg
    10081 gcaacgccta tgaatgggtc caggattggt atgggacaaa ctattatgag atgtcaccgg
    10141 accgtaatcc ccaggggccg gctcaaggcc aattcaaagt cctgcgcgga ggatcctggt
    10201 ccgaccttcc gaagtatctg ctgacgtacg gccgcttcaa actcctgccg gacaccaaaa
    10261 acagttatac aggcttccgc tgcgcgatgt ctgagaagaa gtgatcgatg agagacagtg
    10321 agaaggatgc ggcgtgagca gctgctagta gatgctcttg ctgacttccg aagagagtgc
    10381 gctttcattg ccagacgagt caaaggctga cagggcaaag taataggttt gccctatcgg
    10441 aagacctgag atcgtgtagc tgcccataag gccgatgatc accggggatc cggcgtacgt
    10501 atatcggccc ggcgctgtgc ccacatagag tttatacccc gcgagatcgg cttcactgtt
    10561 cgatgtccag ctcaaggtgg cgctgcctgt tccagacgat gtcgatgccg gcggctgcga
    10621 caaggtcgtc gatggctcgg acgagggcgg cgcagaagat tgccgcggag ctgatggcgg
    10681 cggaggagaa gagatcgtca ccgtcggcgc aatcatttga gagaccggta gttgatcaga
    10741 gagacgttgg agtccgcgtc cgccagtccc atggcttcct gaagaaattg aggaaactgt
    10801 tcctgtgctc gcgaggccgc ccacagcggt cttggtcatg ggggcggcac tcgtcagcgg
    10861 cactgtggtg gaagtgctcg aagctgtcgc gggcgcgctc ggcaccaggg aacctgccga
    10921 tgccaggccg gtcgcaggca gcgaggcgcg gctctcgcta ccggttttct gtatgtctgt
    10981 ggttaccgac agcgtcgtgg cagcagtcgt ctgcggcgtc gcagccaccg ctccaaggat
    11041 cgtggtctgt gcgggtcgtt ttgcgagttt ccgaggcagc ggacgtggag caggggtcga
    11101 tgaggcagca gccgtcggag gcaagctcgt tgccggctcg agcggtcgca gatcggatgc
    11161 ctgacgatgg atcaaactgt gatgtcgggc cgactgctgc tcctccgcga ggagggaggt
    11221 ggcaatgccg attacattca ctgttgcgag gagcgccaca ataatgagga aacgatgaaa
    11281 gtgtgggtga gtgagcatcc gggtcattcc ttgatggaac gtgatcgtgc caggctctgt
    11341 ccggtttatt cagtcgcggt aagggcattc tctatccgaa gccttcaaga ggccagagaa
    11401 aatggatttt gtgcggatgg tctgcgaaga gcggaaaagc agcgacgagg tggtcgagtc
    11461 cgccgtgggg gcccttaaac caagatcaga gcaaagagaa agatgccggt caatccgcac
    11521 agggaggcat agtagtgcag gccggtccgg tcggtcagcc aggccgtttt ccatttggcg
    11581 gggtagagcg tctggtgatc gacaaacagg ccgtcttcat aaaagccgag cgcctgttcg
    11641 agttgaatca gcacctgttt cgccaggcgg tgccgatgct gctgttgcag gaccagcccg
    11701 cagaacagtg cacaccaggt caggctcccg agggccaaca gtacgctttc cggagaagag
    11761 agccgcgact tcacgggact cagaagcagt gcaaagatca tcgcgatcaa gccgaacgca
    11821 ccgaacgccg tcaaccggat catgtgctcg cgccgctgat acacttcatt tttgtagagt
    11881 ggatagagaa cggtgagggt ttgttgaacg ccttccgtga tcatggccgt tccttcaatt
    11941 cgtgcccggg cagtcggtcc gtatcatacc agataccgga cgagccagtc ggtcatggcg
    12001 gtggtcatgg ttctgaaatc ctcgccccgc gtaaactgat ggtccgcctg cggcaagagg
    12061 tccagccgct tcggacctcg cagcgcgtcc aacaagcgcc ggctttgatg aagcggcacc
    12121 agctcgtcgc atgcgccctg gacgattaac gtcggcgcca cgatctgggc ggccggcccg
    12181 taggcaatct gttgaaggga atcttcatac aggccgtact tcaatcggat tcgttccggt
    12241 cctcccatga ggttcggaat cgtgtccgtg acttgccacc gagtcagttc atccggtccg
    12301 aattctaagc gaagttcttc ggcaaaatct accaccgggc atttcaaggc taggcaggca
    12361 atgtccggcc gttgagcagc ggtaaggatc gacaccagcc cgccgaagct cgatccgacg
    12421 aggccgattt ttccaaaacc ccgtgcgtgg accagggtga cggccgcatg ggtttgtcca
    12481 accgccagcg acggcgtgat gtcttcaaaa tggccgtcgc tttcgccctg accgaagaag
    12541 tcgaaccgaa aagtcgcaat ggccttctcg ttcaatagcc tcgtgagcgt cttgttcgtc
    12601 gtactgtttt tgcccgaaag aaagccatgg caaaggacgc agacggcggt ggtaggctgt
    12661 gcggggcgac tcaggatcgc ggagatgcga tgccctcgtt cgtcgtgaaa cgtcagggat
    12721 tcttccatgc aggggcattg tagcagactt cttcggacgg acgtggggag ccggagttac
    12781 attttgctcg atcgcacgcg gtagtcgacc gggtcttgca gccatttcca gaatcgatct
    12841 tgccgtgcga gatcacggtc gggctgcagc ttgtgagatg cgcgattagg aaggccggaa
    12901 gcccacggcc caggtcagct tgacggcaaa caaggtcaga ctcaagatca gcagtccggc
    12961 ggcgatccag acagtgatct gcacggatga agagccaagc ggaacgctcc atgtcagcgc
    13021 ggcaagaacc gccaatgcca tcgtcccgag actcattcca gcgagttgcg ctgttctcca
    13081 ccggttcatg atgccgtcga gttgcgcgcg gtaggcctga cgcttcttgc tgttcggtac
    13141 gcgggtcacc gccaggatat ccggcacgcc tgtcgcgaga ctgccgcaga tgacctgcgt
    13201 cataaacccg atgaacgcga ggtgagtata ggccaccaaa tggagcgatc caatcggcat
    13261 caccggcggc gtgcgcaggt agttggcccc cattgccagg ccagcgacgg tgctgaggaa
    13321 aagaaaacag acaccgagta agagatggtc ggatgcggca tgttccggtg cgccagattt
    13381 gatccagccg acgaggaaat cgtacgtgca catggccacg gcggccacca agagccctcc
    13441 gacggtgatc tccaaccaga gcgatgatgt gacaaatccg ccgagcaagg ccgcgaaacc
    13501 gaccggcaga aaccagagtg cgatgcgggt cagtgcatgg ctggcaagcg gtgcctgcat
    13561 gagaaccgga agaatctgat gcagcgccaa gatgaacgtg accgtcagga atccgagcac
    13621 gaggaaatgg acgtgaaaaa gtcgcagata actctgagcg ccctcgatga tgcggaaggt
    13681 catcagggcg gcggcgatca ggccgagcag taaggcgacc atggccgcgc gagcaatcca
    13741 tactgcaccg gccggctggt acgcgttgcc gatccggagc caggcttttg gtgaaatcca
    13801 caggacaatc ccgatgagca cgagtgctgc caggcctgcc atcgtcatgt ggccgagcca
    13861 aaaactgacg agaaggccga ccgtcgcacc attgagagcc caaaataaag acgagtgaga
    13921 tgtcgcagag gcgtgggtgt gcccgttggc acgagagaag aggaagccgc cgatgatcat
    13981 ttgcaggaag cccccgagca gcgcgccgtg gacgtgcacc ggcttgagcc agtgaggcag
    14041 tggcgtacca ttgaccaagc ccaacagcag ggcggccccc agcagaaacg acactgacag
    14101 ccacatgaat ccggtgagca ggaacgtgag cggcgtaccg gacatcgagg ggcgaacaag
    14161 acgcgatgtc ggacggcgtt cgaggccctc tgactgagag gacaggagtg atcgcttcat
    14221 gattccggta gaaagtagaa atgattggtg ggaccggcac cgtgcccgat cgccaaactg
    14281 tgacggatcg cttcggtcac ataggtcttg gctgccgcga cggcgtccgt gatcgtcttg
    14341 ccacgggcca gttgtgctgc aatcgctgag gcgtaggtgc acccagttcc atgcgtgtga
    14401 ggcgtatcga tgaattcacc tttgaagaca ttgaagaacc ttccgtcata gagaagatcg
    14461 gtcgcccgct cggcgaggag gtgcccgccc ttgatgagca catgcttgga tccgagtccg
    14521 tggatcacct ttgccgcacg gcgagcatcg gccaatgagg tgatctgaat gccggagagt
    14581 tgctgcgcct catggatatt aggcgtgacg agaatcgcga gtgggaacaa ctgctttttc
    14641 atcgcctcga tggcatccgg cttcaagagc gaatgcccgc ttttggagat catgaccgga
    14701 tcgaccacca gattggccac ctcttggggt ttcagcatct tcgcgaccgt ctcgacaatc
    14761 gaatccgacg acaacatgcc ggttttgacg gcagccacat cgaaatcgtc aaagacggcg
    14821 tcgatttgtg ccgcgataat ggaacagggc aagtcgaaca cctccgtcac ttcttccgtg
    14881 ttttgagcgg tgatcgccgt aatcacggac atcgcgaaga ctccgttggc cgacatggct
    14941 ttgatgtcgg cctggatccc ggccccgccg ccggaatccg atccggcaat cgtcaagact
    15001 tgtttgagtg accggctcat ctcggtgagt gggaatcagt gggcatatat atacgttgcc
    15061 acagaagcct gtccagcgtc agcgaaagac caacgtggag agcgcgcgac cgaatgcata
    15121 gcaaatcggg atcaaaagga gcaagaggaa gggcgaaaag agataggcga gcgcggcctg
    15181 ggcgtccgcc tgccagagcg ttgtgagatg cgtccacagg ctgaaggcga ccaggccgat
    15241 cacggtcatg agcacaccgg cgctgcgacg tccgcgaagg gttcggtcgt ccataggagc
    15301 tcggcccaga tgaaacagca agaacacggc gaatatcatg aaaggcatcg cgttgatcag
    15361 cgcaatgacg aagaggttgt atccgggagc aaagagatgc aggcgcagtt gacgaacggc
    15421 tccggcaagt gactggtcgt gcttggccat gtcgacggcg acggtagcaa tccagggaat
    15481 ggccaggccc gacagggtgc aggaccagta caggaccagg gtcaataccg tgaagcgccg
    15541 aggtgaatcc gtcgccgtag aactcatggc cgccgggcct cgatggcctg caatgcctcc
    15601 gcggccgctt cacgcacgat cggatccggg tcatccaggg ttcgttgcag cgcgggcacc
    15661 gcctgcactg cgctgggccc aacggattgg agtgtgcagg ccgccagata acgctcgagc
    15721 ggccgctctg ccgtcaacag cgatgccagc ttgggaacgg cttcggccgc acgaggcccc
    15781 aatttcgtca attgcaacaa gggaagcgtt cggtcgcttc cctgatccag tgagaaaata
    15841 tggaccgaga caggaatcgg atgatcggcc gccgcgaccg ccgattgagc caagcgagca
    15901 accatctcgt tcgggtcgtg cacgaggggc cgcaaagcgg caagcgactc aggattcagc
    15961 actccgccaa acgaaaaggg gctaatggca gcatagcgta cggtccattc cggatcctgc
    16021 agcgcctggg tgagtcctcg tgcgaccagt tcgggaggca gcgcgatcgc cgccaaggcc
    16081 cgcaccacgc ggtcgcgtac gatggtgttg ggatcgtttg cgattccaag caatgaatgg
    16141 accgccggtt tcgccactgg tcccagtgct cccaacgctg ctgcggcgtc gcgtctcact
    16201 tgcggatcgg gatcttgaag cttgggcagc caacgcacca tcaccttccg tgcggc
//
LOCUS       DASSCQ010000176         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_13435, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000176 DASSCQ010000000
VERSION     DASSCQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13435"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFH05_11740"
     CDS             <1..506
                     /locus_tag="VFH05_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET6678937.1"
                     /translation="IIPTTPLPTQGGSADIYGGEAGVEFVLTRWLSGFANYAYQEVGQ
                     TFTGDAKRSPPRFKWNVGMRGDWDRLNAEILYHHVGAATYPINQAFSSFTAFGVVPPD
                     PRVGSYDLLNLRAGYKFWKQKASAGYLREAEVAISAFNALNDKHKEHPLGDTIGSRVM
                     GWLTVRF"
     gene            628..>2451
                     /locus_tag="VFH05_11745"
     CDS             628..>2451
                     /locus_tag="VFH05_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6678938.1"
                     /translation="MVIPGKRYSILVVEDNPDIVIGLQDLLQHDGYDVTVAGTCAAAI
                     ALISQQRFNAILLDLGLPDGDGLDVLREVQRRDPSLPVIIVTAHIAQERTVGSLMKGA
                     FAYLTKPYNREELRQNLRRAIGVKELAVKAERAEQSLTESEHRFQSLVESATDAIVVA
                     NARGIIVSGNRSASRLFGYAVEEMIGQPLTILMPARYREAHERGLARIEATGTGRLIG
                     SVVELHGLRKTGEEFPIELSLATWKTATGSYYSGIIRDISERKKAAHALGKLQRQHTL
                     ILNQAGEGIYGVDERGNTTFVNPSGASMLGYQVDELLGRHMHEILHHSRSDGTPYPVA
                     ECPVYAAIRDGLVHRVVDEVFWRKDGTAFPVEYVSTPITEAGQVVGAVVVFRDTTARR
                     ETELALKASEERLEMVIQGSTDGFWDSQPLPNEPWSSPRTPIWFSPRVKSMLGYTAEE
                     FPDVLESWLNRLHPDDRERVVNAITAHIERHVPYDEEYRLLTKSGDYHWVRARGQAIW
                     DADGRVIRMSGSFQSIMERKRAEDALRRSQQLLQDMADNTIAVIYVKEASGRYILVNR
                     RFEDIFGLKAEQIIGFTDHDIFPPDFADVFRANDVEVLERNA"
BASE COUNT          535 a          716 c          720 g          480 t
ORIGIN      
        1 tgattattcc tacgactccc ttgcccacac aaggtggatc ggccgacatc tatggtggag
       61 aagccggtgt ggagtttgtg ctcacgcgtt ggctgagcgg gttcgctaat tacgcctacc
      121 aggaagtcgg ccaaacattt accggcgatg ccaaacgaag tccgccccgt ttcaaatgga
      181 acgtcggcat gcgaggcgat tgggacaggc ttaacgcaga aattctctac caccacgtgg
      241 gggcggcgac ctatcccatc aaccaggcct tctcgtcctt cacggccttc ggggtcgttc
      301 ctcccgaccc ccgggtcggc agttatgacc tgttgaacct ccgcgccggc tacaagttct
      361 ggaagcagaa agcctcggcg ggctatctgc gcgaggccga agtggccatt tcggcgttta
      421 acgccttgaa cgacaaacac aaagaacatc ccttgggcga cacgatcggc agccgggtca
      481 tgggatggct gaccgtacgg ttctaagtgc tagcattgcg agcgttccca aatatgcaca
      541 gtcgcttttt ctcctacggt ggaggacaat acatgcagca gcgccacgta tctaaagaga
      601 ccttcacatg tctcagctga gggagtgatg gtcattccgg gaaagcgtta ctccatccta
      661 gtggtggagg acaacccgga catcgtcatc ggactgcagg atttactgca gcacgacggc
      721 tatgacgtca ccgtggccgg cacctgtgct gctgcgatcg ccctcataag ccagcaacgc
      781 ttcaatgcca ttctcctcga tcttggattg cctgacgggg acggcttaga cgtcctgaga
      841 gaagtgcaac gtcgggatcc ctccctgccg gtcatcattg tcacggcgca catcgcccaa
      901 gaacgcaccg tcggatcatt gatgaaaggt gcgttcgcct atctcacaaa accgtataac
      961 cgggaggaac tcagacagaa ccttcgacgc gcgatcggcg tcaaggagct cgccgtcaag
     1021 gctgagcggg cagaacagtc gctgaccgaa agcgagcata ggtttcagtc gctcgtcgaa
     1081 tcggcgacgg acgccatcgt cgtcgccaat gcgcgtggaa tcatcgtgtc aggaaatcgc
     1141 tcggcttcac ggctgttcgg atatgccgtg gaagagatga ttggccaacc gctgacgatc
     1201 ctcatgcctg cgcgctaccg tgaagcccat gaacggggat tggcccgaat cgaagcgaca
     1261 ggcacaggcc gcttgatcgg ctcggtggtg gaactgcacg gtctccggaa gacgggggaa
     1321 gaattcccga tcgagttgtc gctcgccacc tggaaaactg ctaccggcag ctactacagc
     1381 ggcatcattc gcgatatctc cgaacgcaag aaggccgctc acgccctcgg caagctccaa
     1441 cgccagcaca cgctcattct gaaccaggcc ggggaaggga tctacggggt cgatgaacgg
     1501 ggaaacacga ccttcgtgaa cccgagcggt gccagcatgt tgggatacca ggtggatgaa
     1561 ctgctgggcc gtcacatgca cgaaatcctg caccattcgc ggtcggacgg caccccctat
     1621 cccgtcgcag aatgccctgt gtatgccgcc atccgggatg ggcttgtcca tcgggtcgtg
     1681 gacgaagtct tctggcgcaa ggacggtacg gctttccccg tggaatatgt gagcactccc
     1741 atcactgaag ccggccaggt cgtcggcgcc gtcgtggtct tccgggatac gaccgcaaga
     1801 agagaaaccg agttggcatt gaaggccagc gaggagcggt tggagatggt catccaagga
     1861 tcgaccgacg gcttctggga cagccagcct cttccgaacg aaccatggag ttcgccccga
     1921 acgccgatct ggttttcgcc acgcgtgaaa agcatgctcg gttataccgc tgaggaattt
     1981 cccgatgtct tggagagttg gctgaaccga ctgcacccgg acgaccggga acgcgtcgtc
     2041 aatgcgatca cggcccatat cgagcgccat gtgccgtacg acgaggagta ccgcctcctc
     2101 acaaaatcgg gtgattatca ctgggtgaga gcccggggcc aggccatctg ggatgccgac
     2161 ggcagggtca ttcgtatgtc cggctctttc caatcgatca tggagcgcaa gcgtgcggaa
     2221 gatgcactgc gtcgcagcca gcaactcctt caggacatgg ccgataacac gattgccgtc
     2281 atctacgtga aggaggccag cggccggtac atattggtca accggcgttt tgaagacatc
     2341 ttcgggctga aggcggaaca gatcatcggt tttaccgatc atgacatctt tccccctgat
     2401 ttcgccgacg tctttcgcgc aaacgacgtc gaggtgctgg agcgaaacgc c
//
LOCUS       DASSCQ010000177        14928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_567981, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000177 DASSCQ010000000
VERSION     DASSCQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567981"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFH05_11750"
     CDS             <1..290
                     /locus_tag="VFH05_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6678939.1"
                     /translation="QNVMERASVLVDGPTVMSTHLPDRLQALADRDSVPAESASFKQA
                     KQQAVESFERNFLIDLLKRNEGHMSNAAREAGVDRKTIERMVKKHGLRGTF"
     gene            complement(332..847)
                     /locus_tag="VFH05_11755"
     CDS             complement(332..847)
                     /locus_tag="VFH05_11755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET6678940.1"
                     /translation="MEFKKIDPRVTITTKFGEIKIRFYPEDAPRHIENFINLIKMEFY
                     NGTTFHRIVPGFVIQGGDPLSKEPDRLRHGSGGPGYWLNPELSDRPHKRGAISMAKTP
                     RDGSSTRDFNDNGSQFFICLADNSGLDRRYSIFGEVVRGMDVVDKIAAVPRDEYNNPL
                     EPIMMTMTVKE"
     gene            complement(957..2249)
                     /locus_tag="VFH05_11760"
     CDS             complement(957..2249)
                     /locus_tag="VFH05_11760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6678941.1"
                     /translation="MINPARTLPIHWLILFLLFCTSVVTYMDRVNISVTARQMMPALG
                     LTQQEMGWVFSAFVVGYALFQIPGGWLADRWGARAVLTAALIWWSVCTAWTALAASSS
                     LADAVGIVGALVTVRFALGMGEAVALPSFNRAVANWVPADSRGFGIGIAIGGIGLGSA
                     VTPPVAAWVMVNWGWQTVFYLSAGLGICMGILWWLLARDRPSDHPWALDRHGVSVTSA
                     GQDGKHPVSWRWLLRSPAIWWLVLSYSCLGYVAYVYLSWFYLYLVNVRGFDVLRGGLY
                     ASAPFLAILIACPLGGWVTDRVAAAYGVTRGRVAAGMSGMILAGCAILVGAMADSATA
                     AVVSLSLGAGCLYFSVGAYWASTTDYSKTHAGLLSGLMNTGANLGGAVSPSLTPWIAA
                     QWGWPVSLATAGGIALIGAALWLKIDPGKGVHVPPDKW"
     gene            2541..5891
                     /locus_tag="VFH05_11765"
     CDS             2541..5891
                     /locus_tag="VFH05_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HET6678942.1"
                     /translation="MSPIRLVVALITLIVVGGVAFLLLSPSLTGDDYLKDFFLRQLEQ
                     NLGRKIEVHRIKLVIFPSIRLEMKDIIIYDRNAKSVMLSAKKLDLVLRLVPLLRKQVV
                     GKRLLVEEPTFTIRRDGSGRWNFLDRDNPLPTDDDEALQMMARIFRIREATIVNGTVV
                     MIDEGRPDGPRTVTLQSVEAALEIYLERGQADLHVAAAHNSSQGHSALSVSGTFKKAE
                     QQTLAVDEGKRLVFPLQFDGLIEVANLSIRDAADFLGPRPVPESLHGRVNARSAIKIF
                     PGVAGYDAVLSDLTGNMEQFAVTGKASLSGLLAPQPTFAVTFSSSPVQISELLDKIPA
                     VWIHSQLPSVMQDRQIKGLVEVVSATVTGSYVEGPQLAVTGEFHVQKGQALLGESRVP
                     AKDLDAHVVIEAGRIRVTKLSGLYGTIHMTDSKGQLSFLEQGPWLELEISGTMAAGDL
                     LQFLSTTVKSELLSRVLTSARDVEGMASPVFRLVGPPNAITFAGGEVKAQHVNLTSSY
                     LPERLTGLQGRFILAEGETQFDQVTGHLGDLLVQVQGGITGGTGSIFRDFAVRISGDA
                     AHMANLMPAKAVPPGTMEGMASTIVVITGASGAPHLRGEIALTDSKVSLPGIMEKPAG
                     APVSVTFEGDVEQSKHMTLTRVEIAAPPIRLPIKGKIQLGDKFFIDASLATGTVSLSS
                     VPEWIVKGGFEAGNLELSLEIKGRERDWHSWKTTGWLALSNGLMVTKGTDGPIHDLYV
                     RLLLTRDTAELKRLSFRLADSDLTMEGTVRNWATKPVMTAKLESNQMDIDLLIPKGQR
                     APIRDVLEWLAATSKVSATASIARGRYKHLKFGAMSARMTIQDGVLDLDRMSGQSTNG
                     DIAGRIVVQLPRGEPAEAEIALRATGLLVEDMYRLAGSKGGGITGEARITGTVRGHGR
                     NPHGIYPTLNGRVDLLLENGRIFKSEERAIWKIISILNLPAVLQGKVDLEKEGLPYNK
                     ITTTVIMRNGLFETENLIIDSPIVKITAAGNYDLPTDQVDMVWAVSPFGSYSQFLKTI
                     PLFGRLFAGERKGFATAMFSVKGAIEDPEVTYLPMKSFATGLTGLAQLAVDVLKNTVM
                     LPIDLVTPDENKAVSKDVIPQEPAPAVP"
     gene            6055..7200
                     /locus_tag="VFH05_11770"
     CDS             6055..7200
                     /locus_tag="VFH05_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET6678943.1"
                     /translation="MSRSSSQSHKATVFIAASELDSNLYHATKFIAPDPFIYLEIKGE
                     RLLIMNDLEMDRAKSQAAVDRVLSYSEIERRARDHGVAAPGSVDIVHVVLREAKIKQV
                     LVPANFPYSHAARLQELGYQVHAKPDPFYEQRVVKTAEEVRHIEEAQRATEEAVAAAH
                     DRLRQALIKEGQLWLDDEPLTSERIKKLINVKLMERDCVAQHTIVAGGEQACDPHNEG
                     SGPLPAHRSIIFDVFPRSASSRYFADMSRTVVRGTPSVELKRLYQTVLDAQEEAIAQI
                     KDGADGMKIHRGICERFEKAGYKTGLVNGRMQGYFHGTGHGVGLDIHEAPRISRTGSL
                     LLEGHVVTVEPGLYYPGLGAVRIEDMVLVTGDGCRNLTNFSKAFELH"
     gene            7269..7703
                     /locus_tag="VFH05_11775"
     CDS             7269..7703
                     /locus_tag="VFH05_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HET6678944.1"
                     /translation="MSYAFRFLDDIAIADIAFEAEGDSAEELFVGATRALLEVLANPV
                     TIADTWQHQVLKREGTLDDLLIEWLSEIVYWKDAAGVVFRDAPLTLRGENGGWTMKAT
                     LIGAPVDGERQELRNDVKGITKHRYRMERNGAMWKVTVVVDV"
     gene            7714..9165
                     /locus_tag="VFH05_11780"
     CDS             7714..9165
                     /locus_tag="VFH05_11780"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET6678945.1"
                     /translation="MRLNTDMRVNRISDEVWEIPVSEKPGMLVPARVYATQSILQSMD
                     RGVFDQVTNVACLPGIQRYALCMPDGHWGYGFPIGGVAAFDAQQGIISPGGVGYDVNC
                     GMRLIRTDLSLMDVQPRLERLMTELFRRVPAGVGAAGAVRLDRSELTRVMLKGARWCV
                     EQGYGWHQDLARIEEGGCISGADPSTVSDQALGRGINQLGTLGSGNHYLEIQVASNER
                     IFDAATATALGITGQDQIVVMVHCGSRGFGHQIASDYLKIFEKAMRRYNITVNDQQLA
                     CAPFRSPEGQDYFAAMNGAANMAFANRQVITHSIREAFADVFGTSAEELGMQLVYDVA
                     HNIAKVERYAEGELLVHRKGATRAFGPSSAELAPEFRQTGQPVICGGSMETGSYLLVG
                     TDRAVQETFGSTMHGSGRTMSRAQAKKQVRGEQVQRLMKERGIMVKAVSLSGLAEEAG
                     FAYKNISEVVETVEGAGITRKVAELRPIGNIKG"
     gene            9202..9639
                     /locus_tag="VFH05_11785"
     CDS             9202..9639
                     /locus_tag="VFH05_11785"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6678946.1"
                     /translation="MIFEKQWRIGAGLAQYGQPFILEEVGHMEQRKNPRFPVRFRSSF
                     TSLNIVGGDGNITDLSLRGCRVESHTAVRTGTSLELRVHVSEDEPPLKVQEGVVRWCR
                     AQQFGLEFVTLEPEEWARLQHTVTQLELHPYQKEPAKDPSEAA"
     gene            complement(9741..10382)
                     /locus_tag="VFH05_11790"
     CDS             complement(9741..10382)
                     /locus_tag="VFH05_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6678947.1"
                     /translation="MSKPRILLADDHLLLLDGLRHLLERHYDLVGVVQDGHSVVSAAQ
                     RLQPDLVLMDVAMPILNGLQAGQRLRERLPNVKLLYVSMYGDTPYVEEALRIGASGYV
                     LKRSGWEELSRAIETVLGGKQYISPELFSALQRPEVQPPTTRDADMRSLTDRQRQILR
                     LVAAGYTAKEIGRMLHITSKTVEFHKGRMMRQLQLQSTVELARYALTHGLSNL"
     gene            complement(10354..11856)
                     /locus_tag="VFH05_11795"
     CDS             complement(10354..11856)
                     /locus_tag="VFH05_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6678948.1"
                     /translation="MNSSGVKKTRSQSWFIAAIAGMTIGIFVLDSFFPLGFVVPALYL
                     IPITLTTRVRYPLAPFVVAVSSTGLTFAGIWTNWTSPVVSLQLGLFNRLLVVLACWMT
                     ATIIWYTTQKYAREELEALVAARTAALRQSETTLQSFFNSTDLMMGVIEVIEDRPVMA
                     ITNRAASKADDCADRAPESLPPALRWASDAPTFWIPHLEAARRTGKTVHVKYEIPSVP
                     PTALSGRIMTSALSFIGYSAAGNGLYAWMGQDITENMHIDQKMEANRRSLEVSQAQLR
                     QLTAQLLTAQDDERRRIARELHDEVNQRVISLAFEIDDQLQQLPELPAAARTALQVVK
                     NEVVELSDQLRELAHRLHPSVLEDLGIASALRGYAQEFEQREHVRVDLTLEEYQRPLD
                     RHLAECLFRVTQEALRNVAKHAGATMVSLALSYHDDRIMLRIEDNGRGLTSHDRNAVQ
                     RGLGLVSMGERVRLLDGTLTLTSDPGSGTCLSVSIPFTGISHVQTTHLTR"
     gene            12238..14367
                     /locus_tag="VFH05_11800"
     CDS             12238..14367
                     /locus_tag="VFH05_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET6678949.1"
                     /translation="MDNRDKPATVLVADDDNAVRQFVRTAIEQIGLNVCEASNGSEVL
                     EQFTLRHPDVIVLDVMMPVMDGFTACSKVRGEPRGGQIPILMMTGLDDAEAIARAYEH
                     GATDFIAKPLNPTMLSQRIRYLLRGSQTVRALLRSEARLGLAQRIAKIGNWEWQPESG
                     QFSASYELCRLIGIRPQDFGGTLDAFLQAVDAEDRERVHHTLTSILPERTPCDIDHRI
                     VLPNGSEFTVNLQAEAVFDDQLNALTIVGTAQDISERKRSEREIHRLAYFDSLTGLPN
                     RVLFKDRVTQAIAHARRYQYNLALLFLDLDRFKATNDTLGHHIGDLLLKHVADRLMDS
                     VRHSDSIGRTTENDRNHELARLGGDEFTVLLTNLRDVQDAGKVARRILEALARPFLIN
                     GHEIFVTVSIGIAIFPTDGDSVDLLLKSSDTAMYHAKEQGRNNFQYYSNAMNAAASER
                     LMLEAEVRQATEREEFVLYYQPQIDLRSGRIVGAEVLIRWLHPQRGLLAPNEFLQAAS
                     DTGIIRSIDEWVLRTACRQARTWQQRGFMVPCVSINVSNSLFHGSTLLSAVQETLDET
                     GLTANRLELELTEPTAMRNLEASLTILHQLKTLGVQLSIDDFGTGYSSLSYLHQLPFN
                     RVKIDHSLIRDMLIKAQPVRIVRAIIAMAHSLQLEVLGEGVEQEQQREILIAEGCDQA
                     QGYLFGHPMPAAEFELHLPTRSFFKAS"
     gene            complement(14368..>14928)
                     /locus_tag="VFH05_11805"
     CDS             complement(14368..>14928)
                     /locus_tag="VFH05_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678950.1"
                     /translation="QPALDALRRFCWEATIWKKDYENGYSGAFLGDGFASPLLFQIAD
                     ELRQKFPRIFSGHRMTQAWAFKHDSTRRGLNIHADAAAVNVNFWITPDEANLDPATGG
                     LVVYDKEAPLEWNFREYNSDRNKPKILAWLKEVGAQAIKIPYRANRAVVFNSDLFHET
                     DDLSFKDDYLSRRINITLLYGYRSKS"
BASE COUNT         3270 a         4164 c         4439 g         3055 t
ORIGIN      
        1 tccaaaatgt catggaacgg gcgtccgtgc ttgtggatgg tcccacagtc atgagcaccc
       61 atttgcccga tcggttgcag gcgctggctg acagagattc ggtgcctgca gaatccgcgt
      121 cgtttaagca ggccaagcag caggcggtcg agtcctttga gcgcaatttc cttatcgacc
      181 tgctgaaacg caacgaaggc catatgagca acgcagcccg tgaagccggc gttgatcgaa
      241 agacgatcga gcgcatggtc aagaagcatg ggcttcgagg gacgttctag cccgcagtcg
      301 tttgtgtctt tctaactgtg cggtttcgga attactcttt gaccgtcatc gtcatcatga
      361 tcggctcgag gggattattg tattcgtccc gcggaacggc ggcgatcttg tccaccacgt
      421 ccattccgcg gaccacttca ccgaagatac tgtaccgtcg atctaatcca ctgttatccg
      481 ccagacaaat gaaaaactgt gagccgttat cgttgaaatc ccgtgtgctc gagccatccc
      541 tcggtgtttt tgccatggag atggcccccc gcttatgcgg gcggtcgctc aactccggat
      601 tcaaccagta gccgggccct cccgatccat gcctgagacg atccggttct ttgctgaggg
      661 gatcgccacc ttggatgacg aatcccggca cgatccggtg aaaggtcgtg ccgttgtaaa
      721 actccatttt tatgagattg atgaagtttt cgatgtgacg cggagcatcc tcgggataaa
      781 agcgaatctt gatctcgccg aacttcgtcg tgatcgtcac acggggatcg atttttttga
      841 actccatcac agggaatcta acaggggagc gctccgggcg gcaagaggga gggaatgggt
      901 gccggccagc gcgatggcga ttccgcacga tctgcgaggg ccgtggccga ccaaggtcac
      961 cacttgtccg gtgggacgtg gacgcctttc cctggatcga tcttcagcca aagagccgcg
     1021 ccgatcaacg ctataccgcc ggctgtcgct agtgaaactg gccagcccca ctgtgccgcg
     1081 atccaaggcg tgagacttgg ggaaacggcc cctccaagat tggcgcccgt gttcataagg
     1141 cctgataaga gtcccgcgtg ggtcttcgaa tagtccgtcg tcgatgccca ataggcgcca
     1201 acagagaagt acaggcagcc agccccgagt gagagactca ccacggcagc ggttgccgaa
     1261 tctgccatgg cgcccaccag gatggcgcac ccagcgagaa tcatccccga cataccggcg
     1321 gccacgcgcc ctctcgtcac gccatacgcc gcggctactc gatccgtcac ccatcctccc
     1381 aatggacaag cgatcaagat ggccaagaaa ggtgcggacg catagagccc tcccctcagc
     1441 acatcgaatc ctctgacgtt gacgagatac agatagaacc acgagaggta gacgtaggcg
     1501 acataaccca ggcagctgta gctcaagacc aaccaccaaa tcgccggact tcgcaggagc
     1561 catctccagc tcacggggtg cttcccatcc tgcccagctg aggtcaccga gacgccatgt
     1621 cgatccagag cccatggatg atcactcggg cggtcgcgag ccagcaacca ccaaaggata
     1681 cccatacaga tgcccagccc cgccgataga tagaacacgg tttgccaacc ccagttcacc
     1741 atgacccagg cagccaccgg tggcgtcacg gccgagccca ggccgatgcc tccgatcgca
     1801 atgccaattc cgaagccccg tgaatcagct ggtacccaat tggcgacggc ccgattgaat
     1861 gagggcagtg cgacggcttc tcccatgccc aaggcaaatc gtacggtcac cagcgcaccg
     1921 acgatcccca ccgcgtcggc aagcgacgag cttgcggcga gcgctgtcca tgccgtgcag
     1981 acggaccacc atatcaaggc tgccgtgagt acagctctcg cgccccagcg gtctgccagc
     2041 catccacctg gtatttgaaa caaggcatat cccacgacaa acgcagagaa cacccagccc
     2101 atttcctgct gcgtgagccc gagagccggc atcatttgac gcgccgtgac agaaatgttg
     2161 acccgatcca tataggtgac gacactggtg cagaacagca ggaaaagaat cagccaatgg
     2221 atgggaagcg tccgagcggg attgatcacg cgatgttgac cgccgatgga gagacgaacg
     2281 gtggcggatt atagcgggtc tttcggattg tcgctagaag aaatgtatgc gcaagaaacg
     2341 aaaatctcct gttggttcgc ggacttcctg gctttcaggg gggtgattcg gtaagatgac
     2401 cgcaggcctg tgcagtcgtg tctctcgtca tgccggatgt cccgcacacg tcggtcgcga
     2461 ggcgccggtt ctgatcgtga caggcacggg tcgcgaacga ttagattgtg ctgagtgttc
     2521 gtccatcgcg gagaaactga gtgtccccga ttcgtctcgt tgtcgccctt atcacactga
     2581 ttgtggtcgg aggcgtcgcc tttctcttgc tgtcaccctc cctgacgggc gacgactacc
     2641 tcaaggactt tttcctccgt cagcttgagc agaatctcgg ccgaaagatc gaggtgcatc
     2701 gtatcaagct cgtcatcttt ccctcgatcc gcttggagat gaaagacatc atcatttatg
     2761 accgcaatgc aaaaagtgtc atgctctcgg cgaaaaagtt ggatctcgtc ttgcgactcg
     2821 tgccattgct gcgtaaacag gtcgttggaa aacgattgct cgtggaagag ccgaccttca
     2881 cgatacgacg tgacggttca ggtcgctgga acttcctcga tcgcgacaat cccttgccga
     2941 ccgacgacga cgaagcgctc caaatgatgg cgcggatctt ccgcatccgg gaggccacga
     3001 ttgtgaacgg tacggtcgtc atgatcgatg agggcagacc ggatggaccc cgcaccgtga
     3061 cattgcagtc cgtggaagcc gcgctcgaga tttacctcga gcgtgggcaa gcagacttgc
     3121 acgtggctgc cgcgcacaac agctcccagg gccactccgc cttgtctgtc tccggcacct
     3181 tcaaaaaggc tgaacagcaa acgctggccg ttgacgaagg caaacggctt gtgtttcctt
     3241 tgcaattcga cggcctgatt gaagtggcca acctcagcat ccgcgacgcc gcggatttcc
     3301 tcggcccacg tcccgttccc gaatccttgc atgggcgtgt gaacgcgcgc agcgcgatca
     3361 agattttccc cggtgtggct ggatacgatg ctgtgctctc ggacctcacg ggcaacatgg
     3421 agcaattcgc cgtgacgggc aaggccagtt tgtccggcct tctcgctcct cagccgacct
     3481 tcgctgtgac gttttcgtct tcgccggtcc aaatcagcga gctcctcgac aaaatccctg
     3541 ccgtgtggat tcattcccaa ctgccgtcgg tcatgcagga tcgtcagatc aaagggctgg
     3601 ttgaggtggt gtccgccacc gtgacgggct cgtatgtcga agggccgcaa ttggccgtca
     3661 ccggagagtt tcatgtccag aagggacagg ccctacttgg cgaaagccgc gtgcctgcca
     3721 aagatttgga tgcgcacgtc gtgattgaag ccggaagaat ccgcgtcacg aaattgagcg
     3781 gcctctacgg gacaatccat atgacggaca gtaaagggca actctcattt ctcgagcagg
     3841 gtccctggct ggaactggaa atcagcggga caatggcggc tggagacctg ctgcaattcc
     3901 tttcgaccac cgtcaagtcc gaactcctgt cacgtgtcct gacgtccgca cgcgatgtgg
     3961 aagggatggc atcgccggtg tttcgtctgg tggggccgcc caatgccatc acgttcgcag
     4021 gaggcgaagt gaaggcgcag catgtgaatc tgacgagcag ttacttgccg gaacggttga
     4081 ccggcctcca ggggcgattc atcctggccg agggagaaac gcaattcgac caggtgaccg
     4141 gccatctggg cgacttattg gtacaggtcc agggcggtat tacagggggc acagggagca
     4201 tcttccgcga cttcgccgta cggatcagcg gcgacgctgc gcacatggcg aacctgatgc
     4261 cggcgaaagc cgtcccgccg gggacgatgg aagggatggc gagcacgatt gtggtgataa
     4321 caggagccag cggcgcgccg catttgcgag gagaaatcgc actcacggac tccaaggtca
     4381 gcctacccgg catcatggag aaaccggccg gtgcacccgt cagcgtcacg ttcgaaggcg
     4441 atgtcgagca atcgaagcac atgacgttga ctcgagtgga gatcgctgcg ccaccgatcc
     4501 gtttgccgat caagggtaag attcaactgg gagacaaatt cttcatcgat gcttcccttg
     4561 cgacgggcac ggtgtccttg tcgtcggtgc ccgaatggat cgtcaaggga gggttcgaag
     4621 ccggaaatct ggagttgtct ctggagatca aaggccgtga gcgagattgg cattcctgga
     4681 aaacgacggg gtggctggct ctgagtaacg gattgatggt caccaagggt accgatgggc
     4741 cgattcatga tttgtatgtg cggctgctcc tgactcgcga cacggctgag ctcaagcggc
     4801 tctccttccg tctcgctgat agtgacctga ccatggaggg aaccgtgcgc aattgggcga
     4861 caaagcccgt gatgacggcc aagttggaat ccaatcagat ggacattgac ctgctgattc
     4921 cgaaaggtca gcgtgccccc atccgtgacg tgctcgagtg gcttgcggcc accagcaaag
     4981 tgagtgcgac ggcgtccatt gcccgcgggc ggtacaaaca tctgaaattc ggcgcgatgt
     5041 cagcacgaat gaccattcaa gacggcgtcc ttgatctcga ccggatgtcc ggacagtcga
     5101 ccaacgggga tattgccggt cgcatcgtgg tccaactccc gcgcggagag ccggcggagg
     5161 ctgagatcgc gttacgcgca accggattgc tggtcgaaga tatgtatcga ttggcgggct
     5221 ccaaaggcgg tggcatcacc ggcgaggcgc ggataaccgg gacggtacga ggccatgggc
     5281 gcaatcctca cgggatctat ccgacgctca acggcagagt cgatctcttg ctcgagaacg
     5341 gtcgtatctt taaatcagag gaacgtgcga tttggaagat tatcagcatt ttgaacctgc
     5401 cggccgtcct gcaagggaag gtcgaccttg agaaggaagg gttgccctac aacaagatca
     5461 cgacgaccgt catcatgcgc aacggcctgt tcgaaaccga gaatttgatc atcgacagtc
     5521 ccatcgtgaa aattaccgcc gccggaaact acgatcttcc gaccgaccaa gttgatatgg
     5581 tgtgggccgt cagccccttc ggatcctatt cgcaatttct caagacgatt ccgttgttcg
     5641 gcaggctgtt cgccggcgag cggaaaggct ttgccacggc gatgttttcc gtgaaaggcg
     5701 ccatcgaaga tccggaggtg acgtatttgc ccatgaaatc tttcgccacc ggcctcacgg
     5761 gcttggcgca attggccgtc gatgtgttga agaacaccgt catgctcccg atcgacttgg
     5821 tcacgccgga tgaaaataag gccgtctcga aggacgtcat tccgcaggaa ccggctccgg
     5881 ctgttccttg accctctacc gaccccgtgt taaattccgc caggataggc caataccccc
     5941 gccggcttcg tttcccatca cgccccacag cgtcgagggt catacaaccg ggcggggagg
     6001 accgccgcgt ggaagagcca cggccgcggt gagtacagtc aggggtccag tcgtatgtca
     6061 cgatcatcgt cgcagtccca taaagccacg gttttcatcg cagccagcga gctcgattca
     6121 aacctctatc acgccaccaa attcattgcc ccggatccgt tcatctacct ggaaatcaag
     6181 ggtgagcgcc tgcttattat gaatgatctt gagatggatc gggcgaagag ccaagctgcc
     6241 gttgatcgtg tattgtccta ctcggagatc gagcgccgtg cgagagatca tggcgtcgcg
     6301 gcacccggga gcgtggacat tgtgcatgtg gtcttgcgcg aggcgaagat caagcaggtg
     6361 ctcgtgccgg ccaattttcc ctacagccat gccgcgcgat tgcaagagct cggctatcag
     6421 gttcatgcca aaccggaccc gttctacgag cagcgcgtcg tgaagaccgc cgaagaagtc
     6481 cgtcacattg aagaggctca acgcgcgacg gaagaggccg tggctgctgc gcatgaccgg
     6541 ctacgccagg ccctgatcaa ggaagggcaa ctatggctgg acgacgaacc gttgacatcc
     6601 gaacgcatca agaaactcat caacgtaaaa ctgatggaac gcgattgcgt ggcgcaacat
     6661 acaatcgtcg ctggcggtga gcaggcgtgt gatccccaca acgaaggaag cggtccattg
     6721 ccggcccatc gcagcatcat atttgatgtc ttcccgcgat cggcttcgtc acggtacttt
     6781 gcagatatgt cgcgcacggt cgtacgcgga acgccgagtg tcgaactcaa gcggctgtac
     6841 cagacggtgc tcgatgcgca ggaagaagcc atcgctcaaa tcaaggacgg cgccgacggt
     6901 atgaaaattc accgtggcat ctgcgagcga ttcgaaaagg ccggatacaa gacgggtctg
     6961 gtgaacggcc gcatgcaggg atacttccat ggcaccggcc atggcgtcgg actggatatt
     7021 catgaagcgc cgcgcattag tcgcacagga tcattgttgc tggaagggca cgtcgtcacc
     7081 gttgagccgg gcctctatta tcccggactg ggcgccgtgc ggatcgaaga tatggtgctc
     7141 gtcacgggcg acgggtgcag gaaccttacg aatttctcga aggcgttcga actccattag
     7201 ccggccgttc atgcccgcca cctgctttct cgcactgtgc agggagcgcc attcccctca
     7261 gcagccccat gtcgtacgcc tttcgcttcc ttgacgatat tgccattgcc gacattgcgt
     7321 tcgaagctga aggcgattca gccgaggagc tctttgtcgg agcgacaagg gcgttgctcg
     7381 aagtcctggc gaatcccgtg accatcgcgg acacctggca gcatcaggta ctgaaaaggg
     7441 agggcaccct agacgatctc ctgattgaat ggttatcgga gatagtttat tggaaagatg
     7501 cggcgggcgt cgtgttccgg gacgcaccgc tcacccttcg tggcgagaac ggcggctgga
     7561 cgatgaaggc gacgctgatc ggcgctcctg tggatgggga gagacaagaa ctccgcaatg
     7621 acgtgaaagg gatcacgaag catcggtaca ggatggagcg caacggtgcg atgtggaagg
     7681 tcacagtggt cgtcgatgtc taagagtggt ccgatgcgat tgaataccga catgcgcgtg
     7741 aacaggatct ccgacgaagt gtgggagatt cctgtctcag aaaaaccggg catgctggtg
     7801 ccggcgaggg tgtacgccac gcagagcatc ttgcaatcga tggatcgggg tgtgttcgat
     7861 caggtcacca acgtggcctg tctgccaggc atccaacgct atgccctctg catgccggat
     7921 ggccattggg gctacggatt tccgatcggc ggcgtcgccg cgtttgatgc acaacaggga
     7981 atcatttccc ctggcggtgt aggctatgac gtcaattgcg gcatgcgcct gatccgcacg
     8041 gatctatcat taatggacgt gcagccgcgc ttggagcgcc tgatgacgga gctcttccgc
     8101 cgcgtgccag ccggtgtcgg cgcggctgga gcggtcaggc tcgatcgcag cgaacttacc
     8161 cgcgtcatgc tgaaaggcgc gcgatggtgc gtcgaacaag gctatggatg gcatcaagat
     8221 cttgcgcgga tcgaagaagg cggttgcatc agcggagccg atccttcgac ggtgtccgat
     8281 caggcgctgg ggcgtggaat caatcaactg gggacgttgg ggtctggcaa tcattatctt
     8341 gaaatccaag tcgcctccaa tgagcggatt ttcgatgccg caacggccac ggcgctcggg
     8401 attaccggcc aggatcagat cgtcgtcatg gtgcactgtg gttcccgcgg ctttggtcat
     8461 cagatcgcca gcgactatct caaaatattt gaaaaggcga tgcgacggta caatattacc
     8521 gtcaacgatc agcaactggc ctgtgccccc ttccgttcgc cggaagggca ggattacttc
     8581 gcggccatga atggtgcggc caacatggcc tttgccaacc ggcaggtcat tacgcattcc
     8641 attcgggagg cgtttgccga cgtcttcggc acgtccgccg aggaactggg catgcaattg
     8701 gtttatgacg tggctcacaa tatcgccaaa gttgaacgct atgcggaggg tgagttgctc
     8761 gtacatcgca agggagcgac gcgtgccttt gggccgtcaa gcgcggagct ggcaccagag
     8821 tttcgacaga cgggacagcc ggtgatttgc ggcgggtcaa tggaaaccgg gtcgtacctg
     8881 ttggtcggaa cggaccgcgc cgtccaggaa acctttgggt cgaccatgca tggttcggga
     8941 cgaaccatgt cgagggccca ggcaaaaaaa caggtccgtg gcgaacaggt tcagcggctc
     9001 atgaaagagc gtggcattat ggtaaaagcc gtgtcactgt cgggcctcgc cgaggaagcg
     9061 gggtttgcct acaaaaatat atccgaggtt gttgagaccg tcgaaggcgc gggcataacc
     9121 aggaaagtcg cggagcttcg accaatcggg aacatcaaag ggtagataga ctgggtctgc
     9181 tggagagagt ttcggcactg tttgattttt gaaaaacagt ggcgaattgg cgccggcttg
     9241 gcacaatacg gccagccgtt cattcttgaa gaggttggcc atatggagca gcggaagaac
     9301 ccacggtttc ctgtccggtt ccggagttcc ttcacatcgt tgaatatcgt cggaggagac
     9361 ggcaacatta ccgacctctc acttcgcgga tgccgagtgg aaagtcatac ggcggtcagg
     9421 acagggacct ctttggaact acgcgtgcat gtctcggaag acgagccacc attaaaggtt
     9481 caggaagggg tggtccgttg gtgccgtgcg caacaattcg gcctggaatt cgtcaccttg
     9541 gaacctgaag aatgggcgcg cctccagcat accgtcaccc agctcgagct gcatccctat
     9601 caaaaagagc cggctaaaga tccctcagaa gccgcctagc ctcctcgaca tccgtacgtc
     9661 tgaaattgac aggacgggtc aaagtgttcc catgcgcccg caataggacg aaggccgtac
     9721 tccgctttcg atacgagtcg ttagaggttg ctgaggccgt gcgtaagggc atagcgcgcc
     9781 aattcgacgg tggattgaag ctgcagctgc cgcatcatgc gtcccttgtg gaactccacg
     9841 gttttcgacg tgatgtgcaa catgcggccg atctctttgg ccgtatagcc ggccgcgacc
     9901 agtcttaaaa tttgacgctg gcgatccgta agcgaccgca tgtctgcgtc gcgtgttgta
     9961 ggcggctgca cttctgggcg ctgtaaggcg gagaacaatt ccggagagat gtattgcttg
    10021 ccccccagga cggtttcaat ggcgcgcgaa agttcttccc aaccggaccg cttgagcacg
    10081 tatcccgacg cgccaattcg taatgcctcc tcgacatacg gtgtatctcc atacatggaa
    10141 acgtagagca gtttcacatt cggcagccgt tctcgcagcc gttgtccggc ttgcaaacca
    10201 ttgaggatcg gcatggcgac atccatgagc acgagatccg gctgtaaccg ctgtgcggcg
    10261 ctgacgaccg agtgtccatc ctgtacgaca cccaccagat cgtaatgccg ttccaggaga
    10321 tgccgcagcc catcgaggag cagcaggtgg tcgtcagcga gtaagatgcg tggtttggac
    10381 atgcgaaatt cctgtgaacg ggattgacac tgacaagcag gttccgctgc cggggtcgct
    10441 ggtaagtgtc agcgttccat ccagaagacg cactcgttcc cccatgctga ccaaccccaa
    10501 gccacgctgg actgcgtttc ggtcgtgtga ggtaagcccg cggccgttgt cctcgatccg
    10561 cagcatgatc cggtcgtcgt gataggaaag cgcaagtgag accattgtgg caccggcatg
    10621 cttcgcgaca tttcgcagcg cttcttgcgt gacacggaag aggcattcgg ccaaatgccg
    10681 gtccaatggc cgctggtatt cttccaaagt caaatcgacc cgaacgtgtt cccgttgctc
    10741 gaattcctgc gcgtaccccc gaagggcgct ggcgatgccg aggtcctcga ggaccgaggg
    10801 atgaagccga tgggcaagct cgcggagttg atccgacaac tcgacgacct cgttcttgac
    10861 gacctgcaag gccgtgcggg cggcggcagg cagttcaggg agttgttgca attgatcgtc
    10921 gatttcaaac gctaaactga tcacacgttg gttgacttca tcgtgcaact cgcgggcaat
    10981 gcgtcgtcgt tcatcatcct gggcggtgag cagttgtgcc gtaagctgtc gtagttgcgc
    11041 ttggctcacc tccaaggaac ggcggtttgc ctccatcttc tggtcgatgt gcatgttttc
    11101 ggtgatgtct tgccccatcc aggcatacag gccgttgccg gcggcgctgt aaccgataaa
    11161 agagagtgcc gacgtcatga tccgtcccga gagcgccgtg ggcggcacgg acggaatctc
    11221 gtatttcacg tgcacggttt ttcccgttcg gcgagccgct tcgagatgcg gtatccagaa
    11281 ggtcggcgcg tcagacgccc accgcaaggc tggaggaagg gactctggtg cgcggtcggc
    11341 gcaatcgtct gccttcgaag ccgcgcgatt ggtgatcgcc atgaccgggc ggtcctcgat
    11401 cacttcgatg acccccatca tcaagtccgt ggagttgaaa aaactttgga gtgttgtctc
    11461 gctttggcgg agcgccgctg tccgggctgc gacgagcgct tccaattcct ctcgggcata
    11521 tttttgcgtg gtataccaga tgatcgtggc tgtcatccaa caggccagaa cgaccaacaa
    11581 tcgattgaac agaccgagtt gaagggagac gacaggcgac gtccaatttg tccagatgcc
    11641 ggcgaacgtc aggccagttg aagagacggc caccacaaac ggcgccaatg gatagcggac
    11701 tcgcgtggtg agcgtgatgg ggatcagata aagggccgga acgacaaacc cgagggggaa
    11761 aaaactgtcg aggacgaaaa tgccgatggt cataccggcg atcgcggcaa tgaaccagct
    11821 ctgcgatcgt gtcttcttga cgcctgacga gttcatagct gtcagggaga tgggatgtcc
    11881 acaaaacgca atggcctgat tataggaggt ttctcgaaca atgagaaagt gaaaaggcag
    11941 ccggatgatg cacctagcaa tactcctagc aacttcgagg gtctcgaacg atgcgaatgt
    12001 gaaaataact agcgattcca agccgttttg ctaggctaag ggctgtccca cacgaaaagc
    12061 ctcaattcac aatttgctcc gtgaatcagt aaccctgagc gcatggaaca gaagtggtgg
    12121 ggaagattgc tgtgaaggga tcagacaaat acttacaaat gtctttggga ggttccgtga
    12181 cggcaacacg tgatgtttcg tgtctgactt catcgcaaga ctcgcgggaa gattcacatg
    12241 gataaccggg acaaacctgc caccgttctg gtggccgacg acgacaacgc ggtcaggcag
    12301 ttcgtccgca cggcgattga gcagatcggg ctgaacgtct gtgaggcttc gaacggcagc
    12361 gaggtgctgg agcaatttac cctccggcat cctgacgtca ttgtcttgga tgtcatgatg
    12421 ccggtgatgg acgggtttac tgcctgctcg aaggtgcgcg gggaacccag aggcggccaa
    12481 atccccattt tgatgatgac cggcctcgac gatgccgagg cgattgccag ggcttatgag
    12541 catggggcga ccgattttat cgccaagccg ctcaatccga cgatgttgag tcaacgaata
    12601 cggtacctct tacgcgggag tcagaccgtc agggcgttac tacgaagtga agccaggctg
    12661 ggtctcgccc agcggattgc gaagattggc aactgggaat ggcagccaga aagtgggcaa
    12721 ttctcagctt cctacgaact gtgccggctg ataggcatcc gaccacaaga ttttggtggt
    12781 acgctggacg cgttcctgca agccgtcgac gccgaggacc gtgagcgggt ccatcacacg
    12841 cttaccagta ttcttcccga gcgaacgcca tgcgacattg atcaccggat tgtgttgccg
    12901 aacggaagtg agttcacggt gaacttgcag gccgaggcgg ttttcgacga tcaattgaac
    12961 gccttgacca ttgtgggaac ggcgcaagac atcagtgagc gcaaacggtc cgagcgggaa
    13021 atccaccgac tcgcgtactt cgacagcctc acaggcctcc ccaatcgagt gctgttcaag
    13081 gaccgcgtga cgcaggcgat cgctcatgcc cggcgctacc agtacaatct ggcgctcctc
    13141 tttctggatt tggaccgttt caaggctacc aatgacacat taggacatca catcggtgat
    13201 ctactgctca agcacgtcgc ggaccgactc atggattcgg ttcgtcacag tgattccatc
    13261 ggtcggacga ctgagaacga tcgcaatcat gagctggcac gtctgggcgg ggatgagttc
    13321 accgtgctgc tgacgaacct acgggatgtc caagatgcgg ggaaagtggc cagacggatt
    13381 cttgaagccc tggcccgccc gttcctcatc aacggacatg aaattttcgt gaccgtgagc
    13441 atcggaatcg ccatctttcc gaccgacggc gactccgtgg atctcctgct caagagctca
    13501 gacacggcga tgtatcacgc caaggagcag ggtcgcaaca acttccaata ctattcgaat
    13561 gcgatgaacg cggcagcgag tgaacgcttg atgctcgaag ctgaggtgcg tcaagcgacg
    13621 gaacgggaag agtttgtcct gtattaccag ccgcagatcg acttgcggag cggccggatt
    13681 gtcggtgcgg aagtgttgat cagatggctc catccgcagc gcggcttact ggcacccaat
    13741 gaatttcttc aggctgcttc cgatacgggc attatccgct caatcgacga atgggtgctg
    13801 cgaacggctt gccgtcaggc gcggacctgg caacagcgcg ggttcatggt cccatgcgtc
    13861 tccatcaacg tatcgaactc attgtttcac ggcagcacgc tcttaagtgc agttcaagag
    13921 accttggacg aaactggtct gactgccaat cggcttgaac tggagttgac ggagcccact
    13981 gcgatgcgca atctggaggc atcgctgacg atcctgcatc aattgaaaac cttgggcgtg
    14041 caattgtcca ttgacgactt cgggacggga tattcctcgc tgagctacct ccaccagttg
    14101 ccgtttaacc gagtgaaaat cgatcactcg ttgatccggg atatgctcat caaggcccaa
    14161 ccggttcgca tcgtgcgcgc gatcatcgcg atggcccaca gccttcaact ggaggtgcta
    14221 ggggaggggg tcgaacagga gcagcaacgg gagatcctga tcgccgaagg ttgcgatcag
    14281 gcgcaaggct acctgttcgg acatccgatg ccggcagcgg agttcgaact gcacctcccg
    14341 acgcgctcct ttttcaaggc ctcctagtca ggacttggag cgatacccgt agaggagtgt
    14401 gatgttgatg cggcggctga gatagtcgtc cttgaaagac aggtcatcgg tttcatgaaa
    14461 caaatctgaa ttgaagacaa ctgcccggtt ggcccgatag ggaatcttga ttgcctgcgc
    14521 ccccacctcc ttgagccatg ccaaaatctt gggtttgttg cggtcgctgt tgtactccct
    14581 gaaattccat tcgagcggcg cttccttgtc atacacaacg agaccgccgg tcgctggatc
    14641 caggtttgcc tcgtcgggag taatccagaa attgacgttc acggcggcgg catcggcatg
    14701 aatgttcagc ccacgcctgg tgctgtcatg tttaaaggcc caggcttgag tcatgcgatg
    14761 gccagaaaaa atccgaggaa acttctgacg cagttcgtcc gcaatctgaa aaagcaaggg
    14821 tgaggcgaat ccgtcgccaa ggaacgcgcc gctgtagcca ttctcataat ctttcttcca
    14881 gatcgttgct tcccaacaaa accggcgcaa tgcgtcgagg gcgggctg
//
LOCUS       DASSCQ010000178         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_490976, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000178 DASSCQ010000000
VERSION     DASSCQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490976"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFH05_11810"
     CDS             complement(<1..754)
                     /locus_tag="VFH05_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678951.1"
                     /translation="MTHEELEEAVPLYATGALDRTERQALEAHLLSGCPSCHNALKEY
                     QSVASLLPFGLSPMQAPRDLKSKIMAARNPDPITDDRVFKDSARPSLEPGEWMNHLFP
                     PIAPARSFSLPWAIGLGILLVVGVGSYFGWTYATRISDDSVKIRQLETSLQQQSDRMA
                     TLQQDLRVRDRTLAELQVDAQRKANDAIELKSQLMEREVELEAIREQLTAAGRGKGQD
                     ELATLLRRPDARVVTLAGSDVARRASAVLLYNP"
     gene            complement(751..1353)
                     /locus_tag="VFH05_11815"
     CDS             complement(751..1353)
                     /locus_tag="VFH05_11815"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6678952.1"
                     /translation="MDTPTRRATSAIDPALLTSVAKGDQQAFSQLYDHSSTLLYTLAF
                     RILGNHEEAADLLQDVYLEVWRKVVRYDVGRGTPIAWLITLTRSRAIDRLRSRNTRGH
                     NATVGLDNPVSSRVADLGPSPFETQADQELRMAVGSAMTILPPAQQQALELAYYEGLS
                     HSEIAARLNQPIGTVKTRIKLGMSKLRDSLRHFWEHGDIG"
     gene            complement(1502..>2368)
                     /locus_tag="VFH05_11820"
     CDS             complement(1502..>2368)
                     /locus_tag="VFH05_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678953.1"
                     /translation="DIDIPSTWNALAEFHIGWEARVAVEAKAMKMAAPALVLADTPYL
                     AASAAKHAGIPGAVVASFTWSDVLDCFAGQSPQQHTILAAIRKAYGDADVGLRIAPGL
                     PMAVVKRVHDIGPIAEPAPSRREELRSCLKLKNAERLVLVGFGGIPLNALPWQAMERL
                     QGHHFIVDRAPASASSRVHPLSSLPFSFKTVLASADLVMTKPGYGTIVEAVALGLPVI
                     YVRRYNFADEAPLVDFLLRHGCGQELSREDFFSGNWQPVLDALTERKAERVRPSVTGA
                     TDAARFLIQYFQ"
BASE COUNT          481 a          687 c          713 g          487 t
ORIGIN      
        1 ccgggttgta caggagcacc gcactcgctc ttcttgcgac gtctgacccg gccagcgtca
       61 cgaccctggc gtccggccta cgcagtaatg tggcgagttc gtcctgaccc ttgccgcgcc
      121 ccgctgccgt gagctgttcc cgaatggctt cgagttcgac ctcgcgctcc atgagctgac
      181 ttttgagttc gatcgcgtcg ttcgcttttc gctgggcatc cacttgcagc tcggccaagg
      241 tacggtctct gactctcaga tcctgttgca gagtcgccat cctgtccgac tgctgttgca
      301 gcgaggtttc cagttgtcgg atcttcacgc tatcgtccga gatccgtgtc gcgtaggtcc
      361 agccgaagta gcttcccact ccaaccacaa gcagaattcc cagtccgatt gcccatggca
      421 gcgaaaacga gcgggcgggg gcgattggcg gaaacaggtg gttcatccat tcgcctggct
      481 caaggctcgg ccgggcggaa tctttgaaga cgcggtcatc cgtaattgga tcagggttgc
      541 gagcggccat gatcttcgat ttcaggtcgc gcggggcttg catgggcgag agaccgaagg
      601 gcaggaggga ggcgaccgac tgatactctt tcaaggcgtt atgacacgac ggacaaccgg
      661 acaagagatg agcctccagc gcctggcgtt ccgttcggtc aagagcaccg gttgcgtaga
      721 gcggtacggc ttcttccagt tcctcgtgcg tcatccgatg tcaccatgct cccagaagtg
      781 gcgaaggcta tcacggagct tcgacatgcc cagcttgatt ctggttttga cggtgccgat
      841 tggttgattc agccgtgccg caatctcact gtgcgacagc ccttcataat atgcgagctc
      901 gagggcctgc tgttgggcgg gcggaaggat cgtcatcgcg ctgccgaccg ccatccgaag
      961 ttcctgatcg gcctgggtct caaaggggct tggacccaaa tccgctacac gtgaagagac
     1021 cggattgtcc agtcccacgg tcgcattatg gcctctcgtg ttccttgaac gtaaccgatc
     1081 gatggcacgg ctgcgagtca gagtgatcag ccaagcgatc ggtgttccac gtccgacgtc
     1141 ataccgtacg acctttcgcc agacttccaa atacacgtcc tgtaacaggt cagcggcctc
     1201 ttcgtgattg cccaatatgc gaaacgccag tgtatacagc agtgtgctgg aatggtcgta
     1261 cagctgactg aatgcctgct gatctccctt cgcgacgctc gtgagcagcg cgggatcgat
     1321 tgcggaggtg gcgcgtcgtg tcggtgtgtc cataaagaag cgctgacctg aatgccaggt
     1381 aggatcactt cacgtccgcc acaactatag catctacgca cagccccgcc aaaaggatgt
     1441 gtattttgat atgtgacctc tcaacgggat atcaaggagt tgctcgatga gtctggaagc
     1501 gtcactggaa gtattgaatg aggaagcggg ccgcatcggt tgccccggtg accgacggcc
     1561 tgacgcgttc ggccttgcgt tcggtcagcg catcgaggac cggctgccag ttgccggaga
     1621 aaaagtcctc acgactcagc tcctgcccgc agccgtgccg aagtaaaaaa tcgaccagcg
     1681 gagcttcgtc ggcaaagttg tagcggcgta cgtagatgac aggtaagcca agggcgaccg
     1741 cttcgacgat cgttccataa cccggtttag tcatgacgag gtccgccgag gcaagcaccg
     1801 tcttgaagga gaagggcaga gacgacaaag gatggacgcg tgaggatgca gacgccgggg
     1861 cacgatcaac gatgaaatga tgaccctgca accgctccat ggcctgccat ggcagggcgt
     1921 tgagcggaat gcctccaaaa cctaccagca ccaagcgttc ggcattttta agtttgagac
     1981 aggaacgaag ctcttcacgt cttgatggcg ccggctcggc aatcggaccg atgtcatgaa
     2041 cccgtttcac cacagccatg ggcagacccg gcgcgatccg taatcccacg tcggcatcac
     2101 cgtacgcctt ccgtatcgcg gcgagtatgg tgtgttgttg aggtgactgg cccgcaaagc
     2161 agtcgagcac gtcggaccag gtgaaactcg cgaccaccgc accgggaatc cccgcatgct
     2221 tggcggcaga agcggccaga tacggcgtat cggcgagcac gagagcggga gctgccatct
     2281 tcatggcctt cgcttcgacg gcgacgcgcg cctcccagcc gatgtggaat tctgccaagg
     2341 cgttccaggt ggaggggatg tcgatgtc
//
LOCUS       DASSCQ010000179         8135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1732377, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000179 DASSCQ010000000
VERSION     DASSCQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732377"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFH05_11825"
     CDS             <1..1140
                     /locus_tag="VFH05_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6678954.1"
                     /translation="KGTAILFAVGAVSGTVLSFELGLLWPRFMERAGPSIGPLFALEG
                     FAFFTEAIFLGIYLYGWSRIPPTVHFLSGLIVVASGIASAVFVVAANAWMNAPSGFVV
                     QEGHLSHIDPVAPLWHPVAWHEAGHLLLGGFAATGFLVAAIHAFWLLREPDSAFHRLA
                     LSIALAIGGPAALLQPLSGDLIAKSVAVHQPAKLASMEALFTTKAGAEFVLGGFPDPA
                     TRTVRYGLLIPNGLSLLLYRDANAQVIGLDHFPPEDWPPVAVVHVAFQIMVGCGLVMA
                     AIATWTGWRWWKSGRLTLDRPWLWALIASGPLGFVAIEAGWVVTEVGRQPWIIDHVMR
                     TADAVTPMPGLWIPMVIFTLLYVVLAGVVLWAIWKHIAATYVSPT"
     gene            1265..2296
                     /locus_tag="VFH05_11830"
     CDS             1265..2296
                     /locus_tag="VFH05_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET6678955.1"
                     /translation="MNGTLEDLLGASLVGALTCYLLTGGADFGAGIWTFFAFGRSGQR
                     QRALIDQAIGPIWEANHVWLIVAVTILFTAFPPAFSLIATGLHIPLSLMLVGIVLRGT
                     AFAVRTHDITSRDGSSSVSPIWGYLFAVSSIITPALLGISVGAVASGRLPVASLPSGS
                     FTERFIAPWLAAFPLSVGLLTTILVAYLAAVYLLVESQDPELRAAFRRRAQAACLALL
                     LTGGYVLVQARQGAPEIYAGLRHTPVGLLAIVVTFGLHLAALVTLWKVRDRAARIFAV
                     AGAVIMLWGWALSQYPYLVEPNLTIADAAPAETLRLLLVSLILGSIILFPLLYYLYRI
                     FKGRVLFDA"
     gene            2346..2597
                     /locus_tag="VFH05_11835"
     CDS             2346..2597
                     /locus_tag="VFH05_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678956.1"
                     /translation="MSTSLVRFLMGTIVLAAVCWSILSWQHPGARGQEAKRYVYKVVD
                     VPPDNTAMQNTLNEYGAAGWELVVIGMGDMTTPRLVFKK"
     gene            3048..5108
                     /locus_tag="VFH05_11840"
     CDS             3048..5108
                     /locus_tag="VFH05_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6678957.1"
                     /translation="MSSVRSVTYPRTIAILRALQLGDLLCSVPAFRALRAAFPKARIT
                     LIGLPWAKAFVQRFTHYLDEFIEFPGYPGLPEQDTDFGRLATFLEYMRARRFDWLIQM
                     QGDGSYVNDLVRLCGARERMGFALPSADGSDLSGFLPYPSDCPEIHRHLRLMEFIGVP
                     SVGDHLEFPLGAQDHAEFKALPEAASLRPGSFVCLHPGGRGLARRWSTQGFAEVAKGL
                     AHRGLQLALTGVAEERSLVEHVARSLPVASVNLAGRTTLGSLGVVLSRSRMLVANDTG
                     VSHVAAALRVPSVILSVGSDPLRWNPLDSSRHRVLGGELTDPQDVLTQVDDLLSRGPT
                     VEPTRVNTADRPLRILTWHVHGNYLFYLTHTPHDWFLPVGFDRPGYSGRAPGFPWSER
                     VKDVPVSQLKRLAFDCILFQSRSAYLEEQFELLTPEQRRLPKVYLEHDPPDDPTDSRH
                     IVNDATMQLVHVTHYNRLMWNNGDTPAEVIEHGVPDPGDVRYSGELPRGLVVINDLPR
                     RGRRVGADLVARIRRTIPLDVIGMNSEAAGGLGEISHDDLPRFMARYRFFFHPVRYTS
                     FGLAVCEAMRIGLPIVAWSVTEMPTVLQDGVSGYLGNDPDRLLQRMALLLADPDEARR
                     LGENAREAARTRFNLERFSADWHRLLTAHTSRHRATRTVPGEAQRTVEPSLNKGTPCA
                     AKLH"
     gene            5087..7018
                     /locus_tag="VFH05_11845"
     CDS             5087..7018
                     /locus_tag="VFH05_11845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6678958.1"
                     /translation="MRGQIALISEHASPLSVLGGVDCGGQNLYVGKVAKALSSLGYEV
                     DVFTRRDDDQLPHMIEWEQGIRVIYVPAGPPRFVRKEDLYPFMQEFTDYMVQFCRGQR
                     KAYDLVHANFWMSGLVAAEMKRRLGLPFVITFHALGRVRRLHQQQADGFPDVRFTVED
                     RLVGEADRLIAEAPQDAGDLIRLYGADPSRIVIVPCGFDPEELWPLDKTSARTTIGLP
                     ADEGMILHVGRMVPRKGIDTVIRAYARFLADYDRPTKLVIVGGESDEPDPERTAEIGR
                     LQQLAADLRVQDHIWFAGRRGRSDLKYYYSAADLFVTTPWYEPFGITPLEAMACGTPV
                     IAANVGGLKFSVRDGETGYLVPPGDHEAIAEKFHLLYSSPPLLQLFGQQAIARVNESF
                     TWMHVGEGLSKIYGEVMLQCGIRQNTAVATSIDRAMDRAIDALCEAKRRLRLPMTEAA
                     VRLGDCLTRGGRVLVYGPSDSADTADEWVTELTGRLISHPGWPVITLRDGGKPAWLTQ
                     DRHLLQQMETLARTDDALLVICTEPVSPSIIACMRQARALGLQRILLSGQEVDQLQDL
                     TDVLLDIPLTDSRHIKHVQKLLLNVLLDLVQEHMAAEDRRTGAHSLPSVLTRARRTKI
                     GANTRRHQLINTTGKTMPP"
     gene            7015..7620
                     /locus_tag="VFH05_11850"
     CDS             7015..7620
                     /locus_tag="VFH05_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6678959.1"
                     /translation="MIPAVFLDKDGTLVEDEPLNVDTSRLRFYPDVFAALRLLQRAGF
                     ALVIVTNQGGVAHGRFSETDVREMRSYLERRLQEAGISLRGFYYCPHAPDGIIESYAI
                     NCLCRKPQPGLLMQAGGELRIDMSQSWMVGDILHDVEAGRWAGCRTILLNNGHETEWL
                     LTESRWPDYIAETLLEAAQLITRSAPHRPGHATPERQEEER"
     gene            7617..>8135
                     /locus_tag="VFH05_11855"
     CDS             7617..>8135
                     /locus_tag="VFH05_11855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET6678960.1"
                     /translation="MTGWKSARNILCVRLDAMGDVLMTTPALQALRHSLPHCRLTLLT
                     SASGAEAGRLAPSIEEVIVYEAPWMKSAAPRESSSAPDHAMAQQLRNRHFDGAVIFTV
                     YTQSALPAAFLCLLADIPLRLAHCRENPYRLLTDWVNELEPDTCIRHEVRRQLDLVAA
                     IGCTGPDDRLTIT"
BASE COUNT         1499 a         2379 c         2453 g         1804 t
ORIGIN      
        1 aagggaaccg ctattttgtt cgcggtcggg gcggtctccg ggacagtgct ttcattcgaa
       61 ctcggtctcc tgtggccgag gttcatggaa cgagcagggc cgtccatcgg tccgctattc
      121 gccctcgaag ggttcgcctt ctttaccgag gccatttttc tcggcatcta tctgtacggc
      181 tggtcacgga ttccgccaac cgtccacttt ctttctgggc tcatcgtcgt cgcgagcggt
      241 atagcctccg cggttttcgt cgtcgccgcg aatgcgtgga tgaacgcgcc gagcgggttc
      301 gtcgttcaag aggggcatct ttctcatatt gacccggtgg cgcctctctg gcacccggtc
      361 gcctggcacg aggcaggaca cctgctgctt gggggctttg ctgcgacagg atttctcgtc
      421 gcagccatcc atgcattctg gctcttgaga gaaccagaca gtgcctttca ccgcctggcc
      481 ctgtcgatcg cgcttgccat tggtggtcct gctgcgctct tgcagccgct gagcggggac
      541 ttgatcgcaa aatcggtggc tgtccatcag ccggccaagc tggcatccat ggaagccttg
      601 ttcaccacga aggccggtgc ggaattcgtg ctcggcggat ttcccgatcc tgcgacacga
      661 accgtgcgct atggactcct gattccaaat gggctcagcc tgctgctcta tcgagacgcc
      721 aacgcccaag tcatcggatt ggatcatttt cctcctgagg attggcctcc tgtggcggtc
      781 gttcatgtcg cgttccaaat catggtgggg tgcggactag tcatggcggc tatcgccaca
      841 tggacgggct ggaggtggtg gaagtccgga cgtctcacgc tcgatcgccc gtggctctgg
      901 gccttgattg cttccgggcc gttggggttc gtggcgatcg aagctggctg ggtcgtgact
      961 gaggtgggcc gacagccatg gattatcgat catgtgatgc ggacagctga tgccgttacc
     1021 ccaatgcccg gactgtggat tcccatggtg atctttacgc tcctctacgt cgtgctcgcg
     1081 ggggtcgtgc tgtgggccat ctggaagcac attgcagcca cttatgtttc gcccacataa
     1141 gggaacgacg agcgccgaat ggccgcttcg acatcaaatg tggaacgaag gaaagaggtg
     1201 cgtttcgaga ccggccctgc tttcagcgac cgggaacctg cggaacaacc ggtgagggtg
     1261 tgacgtgaac gggacccttg aagaccttct aggcgcttca ctagtagggg cgttgacctg
     1321 ttatcttctg acgggaggag cagactttgg cgccggcatc tggacgttct ttgcatttgg
     1381 ccggagcggc cagcggcaac gggccttgat cgatcaagca attggtccca tctgggaagc
     1441 caatcatgtc tggctgatcg ttgccgtgac catcctcttc actgcctttc cacctgcgtt
     1501 ttccctgatt gcgacaggat tgcacattcc gttgtctctc atgttggtcg gtatcgtgtt
     1561 acgaggaacc gcctttgcgg tccggacgca tgacattacc tcacgcgacg gcagcagttc
     1621 cgtctcaccc atatggggat atctgttcgc cgtctccagc atcatcactc ctgcgctgct
     1681 cggcatcagt gtcggagccg tggcctccgg ccggctccca gtggcgtcgt tgccctccgg
     1741 ctcgttcacc gaacgcttca ttgcaccctg gttggcagct tttcccctga gtgttgggct
     1801 cctcaccacg atccttgtgg cgtatttggc tgccgtgtac ctgctggttg aatcgcagga
     1861 tcccgagctt cgagctgcgt ttaggcgtcg agcccaagcc gcatgcctag ccctactgct
     1921 gacgggtggc tacgtgctgg tccaagctcg ccagggggct cccgagatct atgccggcct
     1981 tcgccacacg ccagtcggcc tcctggcgat tgtggtcacg ttcggattgc acctggctgc
     2041 tctggtgacc ctctggaaag tccgcgatcg cgcggccagg attttcgccg tggccggcgc
     2101 cgtgatcatg ctgtggggct gggccctttc ccagtatcct tatctcgtcg agccgaacct
     2161 taccatcgcc gacgctgccc ctgctgaaac cctccgctta ctgctcgtga gtctcatttt
     2221 aggatcgatc attctgttcc cactccttta ttatctctat cgcatcttca aaggacgcgt
     2281 cctgtttgac gcgtaactag agcctgatcg tgagaaaccc aggaaaggcg acgatggagg
     2341 tgagcatgtc tacaagcctc gttcgattct tgatgggaac tatcgtgctg gcagcggtct
     2401 gttggtcgat cttgtcttgg cagcaccctg gtgcgcgagg ccaggaggcc aaacgctacg
     2461 tgtataaggt ggtcgacgtc ccgcccgaca atacggccat gcagaatacg ctcaatgagt
     2521 acggcgccgc cggatgggaa ttagttgtta tcggaatggg ggacatgacc accccgcggt
     2581 tggtattcaa aaagtaacaa cgatagcgtc acctcagaca cactgtctgt gcggggacag
     2641 cttgtcgtgc tgctaccggg ctttgccgcg aggcgtgtgg atcgggaaat gtgacgaacg
     2701 tctcttcctt gaagcgtttc cgtcgtttgg aagcggaagg tctgtgcttt gcacaacgac
     2761 acctgactcc ttttgaagga cagggaggaa ccgagcacat gccaaggaag acagaccgcc
     2821 gccgatcgaa gaaacctagc actcagcgga aaaccgctcc gatgaaagcc tcggaggcac
     2881 gcgggacgac acgaaaaaga gggagttccg ctggcggctc tctcaagcgc ggcagtcgag
     2941 cagtgacctc aaacgatcgt ccgaccaaac gatctcaaac agcacgccgc tcgtcacctc
     3001 gcgcgtagct gttcctcact gggccacacc gaaaatcggt acgttcaatg tcatctgtcc
     3061 gttcggtcac atatccgcga acaatcgcta ttttgcgcgc attgcagctc ggggatttgc
     3121 tctgctcggt gccggcattc cgcgcattgc gcgccgcatt cccgaaggcc cgtatcacgc
     3181 tcatcggatt gccttgggcg aaagcgttcg ttcagcgctt cacgcactat ctggatgaat
     3241 tcatcgaatt cccgggctat cccgggctgc cggaacagga cacggatttt ggtcgactgg
     3301 caaccttcct agagtatatg cgggcgcggc gcttcgattg gctgattcag atgcagggag
     3361 acggctcgta tgtcaacgat ctcgtccgcc tgtgtggagc gcgcgagcgg atgggatttg
     3421 cgctgccctc tgccgacggg agcgaccttt ccggttttct cccctaccca tctgactgtc
     3481 cggagattca tcggcatctg agactgatgg agttcatcgg cgtgccttcc gttggggatc
     3541 acctcgagtt tcccctgggt gcacaagatc atgccgaatt caaagcgctt cccgaggccg
     3601 cgtccttacg gcccggctcg tttgtctgtc tccatccggg aggcagaggg ctcgcgcgtc
     3661 gctggtcgac tcagggcttt gccgaggtcg cgaagggatt ggctcatcgg ggtctgcaac
     3721 tggccttgac gggagttgca gaggaacggt cgctcgtaga acacgtcgct cgttcccttc
     3781 ccgttgcctc cgtgaatctg gcgggcagga cgacgctggg gtcgctcgga gtggtattga
     3841 gccgctcccg catgctcgtc gccaacgaca ccggggtgtc acatgtggca gcggctctcc
     3901 gagttcctag tgtcattctt tcagtcggtt ctgatcctct gagatggaat cctctcgata
     3961 gctctcggca tcgggtgctg gggggagagc tgaccgaccc tcaggacgta ctgactcagg
     4021 ttgacgatct cctcagccgc gggccgaccg ttgaaccgac gagggtgaat acagccgaca
     4081 ggccgctgag gattttgacc tggcacgtgc atgggaatta cctcttttac ctcacccata
     4141 ctcctcatga ctggttcttg cctgttggct tcgatcgccc aggatacagc ggccgagcgc
     4201 cgggatttcc ctggtccgag cgagtaaagg atgtgccggt ttcgcaactg aaacgcctgg
     4261 cctttgactg cattctgttt caatccagat ctgcttacct cgaagagcaa ttcgagctcc
     4321 tcactccgga gcagcgcagg ctgccgaaag tgtatctcga acatgacccg ccggatgatc
     4381 cgacggattc gcgccatatc gtcaatgatg caaccatgca gcttgtgcac gtcacgcact
     4441 acaaccgcct catgtggaac aacggtgaca cgccggccga ggtgattgag cacggcgtgc
     4501 cagacccggg agacgtccgt tacagcggcg aactgccccg tgggttggtc gtgatcaatg
     4561 acttgccgcg gcgaggccgt cgagttggcg ccgatctcgt ggcacgcatc agacgcacga
     4621 ttccgttgga cgtgatcggg atgaactcgg aagcggccgg cgggctgggg gagatctcgc
     4681 atgacgactt gccgcggttc atggcgcgtt atcggttttt ctttcatccc gtgcgctaca
     4741 caagtttcgg tcttgccgtc tgcgaggcga tgcgtatcgg ccttccgatc gtggcctggt
     4801 cggttaccga aatgccgact gtgctgcagg atggcgtttc gggctatctc gggaatgatc
     4861 ccgaccggct gctgcaacga atggccttgt tactggccga tccggatgaa gcccgacggc
     4921 tgggtgagaa cgctcgcgaa gcggcacgga cccgcttcaa ccttgaacga ttcagcgctg
     4981 attggcaccg cctgctgaca gcgcatacat cccgtcaccg tgcgacacgg acggtgccgg
     5041 gcgaggcaca gaggacagtc gagccgtcat taaacaaagg aacaccatgc gcggccaaat
     5101 tgcattgatc agcgaacatg cctcccctct cagtgtactg ggtggtgtcg actgcggcgg
     5161 ccagaatctc tacgtgggca aggtggctaa ggcactgagc tcactcggat acgaggtcga
     5221 tgtgtttacg cggcgtgacg acgatcagtt gccgcacatg atcgagtggg agcagggcat
     5281 tcgtgtgatc tacgttccgg caggaccgcc tcggtttgtc cgcaaagagg acctttatcc
     5341 gttcatgcaa gagttcaccg actacatggt ccagttctgt cggggacagc gaaaagccta
     5401 cgaccttgtc catgcaaact tttggatgtc aggtctcgtg gctgcggaaa tgaaacgtcg
     5461 tctgggcctt ccctttgtca tcacgtttca tgcgctgggt cgtgtgcggc ggttgcatca
     5521 acagcaggct gacggcttcc cggatgtgcg atttaccgta gaagaccgtc tcgtcggcga
     5581 ggccgatcgg ttgatcgcag aggcgcctca ggacgccggc gatctcatcc ggctctatgg
     5641 cgccgatccg tcacgtatcg tgatcgttcc gtgcggcttt gatcctgagg agctttggcc
     5701 gctggacaag acctccgcgc ggacaaccat cggcctgccc gccgacgaag ggatgattct
     5761 ccatgtcggg cgcatggtgc cgcggaaggg catcgatacg gtgattcgag cttatgcccg
     5821 ctttcttgcg gattatgacc gtccgacgaa actggtgatt gtcggcggcg agtcggatga
     5881 acccgatcca gagcgcacag cggaaatcgg ccgcttgcag cagctcgcgg cagatcttcg
     5941 cgtgcaggat cacatctggt tcgcgggacg gcgcggtcga agcgatttga aatattacta
     6001 cagcgcagcc gatctcttcg tgaccactcc ctggtacgag ccgtttggca ttacgccgtt
     6061 ggaagcgatg gcgtgcggga ccccagtcat tgccgcgaac gtcggcggcc tgaaattctc
     6121 cgttcgtgac ggggagacag gttatctggt gccgccgggc gatcatgaag cgatcgcaga
     6181 gaaattccac ttgctctatt cgagcccgcc gctgttgcag ttgttcggtc aacaggccat
     6241 cgcgcgggta aacgagtcgt tcacatggat gcatgtcggt gaagggttgt cgaagattta
     6301 cggcgaggtc atgctccaat gcggcatcag acagaatacc gctgtggcga cctccattga
     6361 tcgagccatg gatcgagcca ttgacgcgct gtgtgaggcg aagcgccggc ttcgtcttcc
     6421 tatgaccgaa gcggcggtgc gattgggcga ctgtttaacg cgtggtggac gtgtgctcgt
     6481 ctatggaccg tccgacagcg ccgacaccgc ggatgaatgg gtcactgagt tgacgggccg
     6541 cctcatcagt catccaggtt ggccggtcat tacgctgcgg gatggcggga agccggcatg
     6601 gctcactcaa gatcgccacc tgctccagca gatggaaacg ctggcacgca ctgacgatgc
     6661 cctgcttgtc atctgtactg aacctgtctc gccctcgatc atcgcctgca tgagacaggc
     6721 gcgtgcactc ggtctccaac ggattctcct ttcgggtcag gaggtggatc aactgcagga
     6781 tcttaccgac gtgttgctcg acattcccct gacggatagc cggcatatca agcacgtcca
     6841 gaagctgctc ctgaatgtgc tcttggatct cgtgcaagag cacatggccg ccgaggatcg
     6901 acgaacaggt gcgcattccc tgccatcggt cctcacgaga gcacggcgga cgaagatcgg
     6961 tgcaaacacg cgtcgacatc aactgatcaa cacgactgga aagacaatgc ctccatgata
     7021 ccggcagtat ttctcgacaa ggatggcacg ctcgttgaag acgagccgct gaacgtcgac
     7081 acgtcgcggc ttcgtttcta tcccgatgta ttcgcagcct tgcggttgct tcagcgggcg
     7141 ggatttgccc tcgtcatcgt gacgaaccaa gggggcgtcg ctcacggacg tttcagcgaa
     7201 accgacgttc gggaaatgcg ctcctatctc gaacgtcgtt tgcaggaggc gggcatctcg
     7261 ctgaggggct tttattattg ccctcacgct cccgacggca ttatcgagtc ctatgcgatc
     7321 aattgtctct gtcggaaacc tcaacctggc ctgctcatgc aagcgggtgg cgagttgcgc
     7381 atcgacatga gtcaatcctg gatggtgggg gacatcctgc atgacgtcga ggccggtcgt
     7441 tgggccgggt gccggacaat tctgttgaac aacgggcacg aaaccgaatg gctgcttacc
     7501 gaaagccgat ggcctgatta tattgccgag accctactcg aggcggcaca gctcattacc
     7561 cggtccgctc cccacaggcc ggggcacgcg acgcctgaac gtcaggagga ggagcgatga
     7621 ccggctggaa gtcggcgcgt aacatcttat gcgttcgcct cgacgcgatg ggcgacgtct
     7681 tgatgacgac accggctctg caagcgttgc ggcactcgct tccgcattgc cggctcacgc
     7741 tgttgacatc ggcgagcggc gctgaagcag gccggcttgc gccgtcgatc gaagaggtca
     7801 tcgtctacga ggctccgtgg atgaaatcag cagcgccgag agagagcagc agcgcccccg
     7861 accacgcgat ggcacaacaa ctgaggaatc gacatttcga tggggcggtg atctttacag
     7921 tgtacacaca aagtgccttg cccgcggcgt tcctctgtct gctcgccgac attcccttgc
     7981 gtctcgccca ttgccgtgaa aatccgtacc gcctcttgac ggactgggtc aacgagcttg
     8041 aacccgacac gtgcatacgg cacgaggtcc gccggcagct cgatttggtc gcggcgatcg
     8101 ggtgcacagg tcctgatgac cgcctgacca tcaca
//
LOCUS       DASSCQ010000180        14305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_701925, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000180 DASSCQ010000000
VERSION     DASSCQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14305
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701925"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1168)
                     /locus_tag="VFH05_11860"
     CDS             complement(41..1168)
                     /locus_tag="VFH05_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6678961.1"
                     /translation="MQPSLRLGRLGGIEIGIHYTWLLIAVLLTGSFFGYFSNTYAHWP
                     LRTTWSAAIVISVLFFASIILHEMAHALVAKAYGMQVRTITLFALGGVAHIEKQAATA
                     AAEFWMGLAGPVMSAAVGFLCGWLGSWLGASAQGGFTPVAAVLLSLSSLNLMLAMFNL
                     IPGFPLDGGRILRAIIWGLSGDENRATRIASRAGQGVALGFFGLGLWEFLGAESLSGL
                     WLGLLGWFLLDAATASYVQAEVLTGLRGMRVSELMSNDCMRVPGDMPLQRFADEYVLK
                     TGRRCYAVEDRDTIIGLITAADLTKVDSSRWTQIPVMQVAHPLAEVRSVSPDTPVAEA
                     LQTMGREDLNQLPVVEAGRMEGMLSRGHILRALQTRSELSM"
     gene            complement(1203..1679)
                     /locus_tag="VFH05_11865"
     CDS             complement(1203..1679)
                     /locus_tag="VFH05_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6678962.1"
                     /translation="MATAVCPGGCAMLDSGYLNLLGRVLLGLIFLISGINKIADPQGT
                     QHYMTLMGMTTMTTLFYIGAVAIEIAGSLSLLLGYRTKAGAWLLFVFMIPTTLVFHTN
                     FGDQNQMIHFLKNLAMMGGLLYAAAYGGGRLSMDAGLERVERREEPRRPLRKVVHQ"
     gene            complement(1688..2548)
                     /locus_tag="VFH05_11870"
     CDS             complement(1688..2548)
                     /locus_tag="VFH05_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678963.1"
                     /translation="MHQNAQDQVLGYNGSLQSVGLPADPAHEVYREALSALSKAQIPF
                     LVGGAFGLESYTGLVRRTKDLDLFVRPGDRMELMQRLSEAGFQTEWRFSHWLGKAHKD
                     NRSIDVIFNSGNGVCTVDDAWFRHALPAEILGLHVEVVPAEEMLWSKAFVMERNRYDG
                     GDIAHLLRARAAVLDWARLLFRFGVHWRVLFSHVVLFGFIYPEQRLRIPDWFMHEMIR
                     RLKLDMQCLPSDRRVCQGTLLSWSQYLVDMDRGDYEDARQMPRGRLSPEETQYMTDVL
                     QREEKDAQEH"
     gene            complement(2572..3345)
                     /locus_tag="VFH05_11875"
     CDS             complement(2572..3345)
                     /locus_tag="VFH05_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6678964.1"
                     /translation="MDNRDVKETGVIRVAAVGDLHCPRTSADELRELFENLANEADVL
                     LLCGDLTDYGKPDEARVLADHLRQVKPVPIIAVLGNHEYECGKHDEITAILTEGGATV
                     LDGTAIEVLGVGFAGIKGFCGGFGARALQPWGETVLKQFARESVEETVKLESALAKLR
                     TASKVVLMHYAPVVDTVRGEPPEIFAFLGSSRLEEPLNRYEVTAVFHGHAHNGSLEGR
                     TLTGTPVYNVALPLLNKQFPGRRAVRLIEVPSAAAPVAS"
     gene            complement(3358..4335)
                     /locus_tag="VFH05_11880"
     CDS             complement(3358..4335)
                     /locus_tag="VFH05_11880"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6678965.1"
                     /translation="MTYSPAAALSGVELWDAVIVGGGPAGLSAAVYLGRSRRRVLLFD
                     LGESMAKWEPEVENYLGFPEAITGQSLLERGRAQVRRFGVPVVEGRVEGLTRQSDRFV
                     LASGPATYRSRRVLLATGLTHLLPEIQGAQACLGRTVFFCKDCDAYRVQGKRIAIYGH
                     RNEAARYALAMLAFSPFVTIVTDGRRPVWDVAWGAALAEYGVPVRAERIKELSHRDGR
                     LAAVVFEEGPSCEVQAMFTTRGDVFHTGLAEGLGAAEDEEGQLIVDADMRTTISGLYA
                     AGCVTPANCQLIIAAGQGATAAQAIDRDLFAENLRRHDLPRFGGDFVST"
     gene            complement(4373..4705)
                     /locus_tag="VFH05_11885"
     CDS             complement(4373..4705)
                     /locus_tag="VFH05_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET6678966.1"
                     /translation="MRNGDYEYYEDDAMGWSAFVAGAFIGAGLALLFAPQTGTELRGM
                     LRNYAARAKDEAWERGRTAWDSAVERGKEYYEKGQEAVQDAGRSAREYVQSGKESIRE
                     GREGSHRG"
     gene            5065..6861
                     /locus_tag="VFH05_11890"
     CDS             5065..6861
                     /locus_tag="VFH05_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6678967.1"
                     /translation="MPSKRMYPADNRRSSVYLLQRAWPFIAPFRGQLTCLCFVVLLSI
                     PLSLLTPLPLTLAVDSVLGTKPLPVWLQAWLPPTVSSSPGSLLFFVCAAYIGVALLIH
                     VQSMVLWLLSSYTGERLIYAFRQRLFEHLQRICASYHEAHGPTDSVYRIQHDAASVKQ
                     IPIDAFLPFVKACCLLSGLAALMLLIDAEVAAVALTMLPVLFWLTHRCGRRLRQQWTD
                     VKSTETATVACAQEVLSASRLVKAFGREEHEQRRFLAHAMNWVRQHNALASICSGFDF
                     LFGMTITIGTAIALFIGISHVKGGRLSLGDLLLLMAYMAQLAGPLDTAAKKLTELQSH
                     LVGFRRALAILDTPPLVTDRPASTPLNKAKGHVAFRGVSFAYSSSAAVLRDVSFHLPA
                     GTRVGIVGPSGAGKSTIVNLLTRFHDPTSGAVLLDGTDLREYRLTDLRKQFAIVPQDP
                     QLFSTTIAENIRYGDLLATNAQVEAAAEAAHAAGFIRSLPDGYETPVGERGSRLSGGE
                     RQRIALARAFLRDAPIVILDEPTSALDAGTEKDLIEVMERLTSGRTTLMIAHRLNTLR
                     HCDFQLVLQQGRVIVQAPDWALQSPSGDFVSV"
     gene            complement(6858..7172)
                     /locus_tag="VFH05_11895"
     CDS             complement(6858..7172)
                     /locus_tag="VFH05_11895"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HET6678968.1"
                     /translation="MTQTRGKSGKAKAVFKRSKSEAPSETASRERQEPGRSSSTDSRR
                     DETSSEQDPHVIARIQQRAYWLYEASGFEEGRALEHWLEAERQVTGSAQRQSDPPSTE
                     QS"
     gene            complement(7286..7999)
                     /locus_tag="VFH05_11900"
     CDS             complement(7286..7999)
                     /locus_tag="VFH05_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678969.1"
                     /translation="MNWTQGIGRGVLSMLLVGACAGSTFAEQQSAGKGVGEGETSQQK
                     SQQGIGVEGGGSSNVLLGGPEIIIGKIEQVQGEEYSIQGDHGQSVKLRLTKDTNMVCA
                     GGEGAKMSTGKQDPKEGHEIPISPATEGQMQSHDASKAQQQMQALNDPNQQQTEHQMP
                     APSKDPSQLKDVVGSTDEAANKDIARGSGFSVGGSSGCQFKQGDMVRVEASDMGTITT
                     IKALTDQAGSAIAGKGQEQ"
     gene            8790..10049
                     /locus_tag="VFH05_11905"
     CDS             8790..10049
                     /locus_tag="VFH05_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6678970.1"
                     /translation="MRRWFIVPFSALLIVVFNAGAWADVSLPTYALADIIGIAVERNP
                     LVAGAQAAIDQSTGQRTAAGAYPNPTLHGDGGKGYLRDAGPLTNEIPRSANEYMAGFS
                     QPIEWPAKRAARQRAAEAGLAGASVGLVETRLNLTADVKVAFYDLLLAQRDLDLAKNN
                     LAIVEDVRRIVGVRVRLGEAPQFDLIKAEVEVLKANQAVTRAENTVRVNRVILDTFTA
                     GALGPTYAVSGDFLMFPAGLDLGQLATRSIREHPSIQRLTRSIDQADRTVEFERQSRV
                     PNMNLNTSYTREYGREGFDVGLSVPAPLWYQRQGEIAGSLGAKRREEAELLRTRNELL
                     RQVNQYFQDAQTTAKLIEVLEKGLLKQAEEALRIAKFSFQQGAASLLEVLDAQRVQRQ
                     TLFDYAQARFELSVALARLERAVGGLL"
     gene            10123..11319
                     /locus_tag="VFH05_11910"
     CDS             10123..11319
                     /locus_tag="VFH05_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6678971.1"
                     /translation="MFDLISRLTQSGCLLMVLCTIGCNSAAEESPGQHSTPKAHETGI
                     VTLTAEEIRSGAVVVEPAARGEFKLHRDFPATVVPDHHATADITALVRGRVVDVYADL
                     GQQVKGGDLLAMMYSSDLGMAQSAYLKAIAKLYVAERAYERATMLLREKVIGLAESQR
                     RQGEMLSLRAEKRESEDRLRLLGMSEEQIAKLNKEQKILSYVPITAPFDGRVIARNLT
                     KGEVVEVTEKLFTVADLSEVWVLANIPEKDITFIRADGESERSGVEQLVEVLVTAYPG
                     EVFHGKVTYVGDVLDVATRTMNLRLELPNPQRKLKPEMYATIRVYSLPEPGVLIVPEA
                     AVQRERDRRFVFVQRDAHTFEARDVKLGDGNGDVVKVLDGLQEGEPVVVKGAYVLKSE
                     LLSDQI"
     gene            11399..>14305
                     /locus_tag="VFH05_11915"
     CDS             11399..>14305
                     /locus_tag="VFH05_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET6678972.1"
                     /translation="MSAFLELALRQRVLVLALACLMAAGGIYSFRTIAIDAFPDVTTV
                     LVQVVTEAQGLSPAEIERFVTFPLELQLMGAPGLTDIRSLSKVGLSMITVVFRDDVDI
                     YLARQVVLERVLEVQDSLPPGARSELVPNSPGLGEVYQFYLEGPHDNDADYKLTETEL
                     MERRTLEDWIIRPLLKGTPDVVDVNSIGGFVKQYQVMVEPGLLRKYDLALHDVFMAVA
                     NNNANAGGNILEKGSEKYIVRGIGLIKTLQDIENIVVKEARGTPVYVRDVAEVQIGHA
                     VRHGAAVVNGKREVVAGIVLLLRGGNAREVVEAVKEKIDEIHEQKLLPGGLRIVPFYD
                     RLELIVAALHTVYKALLEGIVLVILVLFLFLGNVRSALIVTATLVVAPLATFIVMDQV
                     GLTANLMSLGGLVIAIGMIVDGSVVVVENVYRHLSEHAPVSSTSRGVIVLKATKEVAQ
                     PVIFGILIIILVFLPILSLQGMEGKMFKPLAYTIMIALLVSLLLSLTLSPALCSLALT
                     TATEEDTWLLRQAKRWYSPALTWSLAHRRMILTSAVTVLGLSLMLFPFLGGEFIPILN
                     EAAITPQTIRLPSISLEKSIEIELEMQRAVMEFPEVRMVVSKIGRSELGNDPQEPNAS
                     DPVVSLKPMDEWTTAKTKPELDDAVRRRIERVPGANFLLSQPIQQRVDELLSGVRSEA
                     TVKIIGEDLEQLRKMAEQVQAIMAGISGVKDVRVEQLFGQAYLSIDIDRGKIARYGIN
                     VAHIREIISTAIGAEAATRVYEGQKRFDLILRYPKKYRDSVETISNILLRASTGALIP
                     LGELAKVELREGPALISREGLQRRIYVGFNTLGRDIESIVGEAQAKIAKQVQLPTGYR
                     LIWGGSFENMQRAMARLKIIVPITIGLIFLLLFASFNSARHAALIIMNLPFALIGGIV
                     ALWVTGEYLSVPASVGFINLFGVAVLNGIVLVSYINQLRAEGLPDHEAIVRGCLL"
BASE COUNT         2887 a         4344 c         3988 g         3086 t
ORIGIN      
        1 aagctgttcg tccgtgcatg ctgacctccc tgtggtggca ttacatactc aattcgcttc
       61 gggtctgcag ggctcgcaaa atatggccgc gcgacagcat accttccatt cgtccggctt
      121 cgacgactgg cagttggttg aggtcctccc gtcccatggt ctgtaacgcc tctgcgactg
      181 gagtatcggg agacaccgac cggacttcag caaggggatg cgccacttgc atcaccggaa
      241 tttgcgtcca cctagaactg tcaacctttg tcagatccgc agccgtgatt aatccgatga
      301 tcgtgtctcg gtcctccacg gcataacatc gccgccccgt tttcagcaca tattcatcgg
      361 caaaccgctg cagcggcatg tcaccgggca ccctcataca atcgttggac atgagctcgc
      421 tcacccgcat tcctcggagg cccgtcagca cctccgcctg gacgtaactg gcggtcgccg
      481 cgtccagcaa gaaccatccc agcaatccaa gccagagacc gctcagactc tcggctccga
      541 ggaattccca taatcccagc ccgaagaaac ccaatgccac tccctgtccg gcgcgcgagg
      601 cgattcgagt ggcgcgattc tcgtctccgc tgagtcccca aataattgct cgtaatattc
      661 tgccgccatc caatgggaag cccggaatca aattgaacat ggccaacatc aagttcagtg
      721 aactcaacga gagcaatact gccgccaccg gcgtaaatcc accctgcgct gatgcgccca
      781 accaggaccc tagccatccg cataggaagc cgaccgccgc gctcatgacg gggccggcca
      841 gtcccatcca gaactcagca gcagccgtgg cggcttgctt ctcgatgtgc gcgaccccgc
      901 ccaacgcaaa gagcgtgatc gtgcgcacct gcatcccata agctttcgcc acgagggcgt
      961 gagccatctc atgcaggatg atcgaggcga aaaaaagcac gctgatcacg atagccgccg
     1021 accaggtcgt tcgcagcggc caatgcgcat aggtattgga gaagtacccg aagaacgagc
     1081 ccgtaagcag caccgcaatc aggagccacg tatagtggat gccgatctct atgccaccca
     1141 gacggccgag tcgtaatgac ggttgcacgc gcgtgtcctc gtttgcctgt ttagtgactt
     1201 gcttactggt gcacgacttt cctcaagggg cgacgcggct cttctcttcg ctctacgcgt
     1261 tctagccctg catccatgct caagcggcct cccccgtacg cagcggcgta taacaggccg
     1321 cccatcatcg cgagattctt caggaagtgg atcatctggt tctgatctcc aaagttggta
     1381 tgaaagacca gcgtggtagg aatcatgaat acaaacagga gccacgcacc cgccttcgta
     1441 cggtacccga ggaggagaga aagagaaccc gcgatctcaa tggcaaccgc tcctatataa
     1501 aatagggtgg tcattgtcgt catgcccatc agggtcatgt agtgttgagt cccttgggga
     1561 tcggcgattt tattgatgcc tgaaatgaga aagatgagac cgagaagcac tcggccgagc
     1621 aaattgaggt aaccggaatc gagcatggca cagcctcccg gacagacggc agtggccatc
     1681 ggcccaccta atgttcttgt gcgtcctttt cttcgcgctg cagcacgtcg gtcatgtatt
     1741 gagtctcctc cggtgacagt cgtcccctcg gcatctgcct ggcgtcctcg tagtccccac
     1801 gatccatgtc aaccagatat tgggaccacg acagcagtgt gccctgacaa acgcgccggt
     1861 ccgaaggcag gcactgcatg tccaatttca gccgtcggat catttcatgc atgaaccagt
     1921 caggtatccg taaccgttgt tcggggtaga tgaagccaaa gagcaccaca tggctgaaca
     1981 ggaccctcca gtggacaccg aaacggaaca gcaatcgggc ccaatcaagc acggcggccc
     2041 gagcgcgcaa cagatgtgcg atatcgccgc cgtcgtaccg gttgcgctcc atgacgaacg
     2101 cctttgacca cagcatttcc tctgcgggaa caacctctac atgtagaccg agaatctcag
     2161 caggcaacgc atgccggaac cacgcatcgt cgaccgtgca cacgccgttg ccggagttga
     2221 agatgacatc gatgctgcga ttgtctttgt gggccttgcc gagccagtgg ctgaaccgcc
     2281 actccgtctg aaatccggcc tccgagagcc tctgcatgag ctccattcga tcgcctggac
     2341 ggacaaataa atccagatct ttggtgcgcc ggaccagccc cgtataactt tccaatccga
     2401 acgccccgcc gacgaggaag gggatctgcg cctttgacag agccgacagg gcttcacgat
     2461 acacctcatg ggccgggtcg gcaggaagac ccacggactg aagcgagccg ttgtatccga
     2521 gtacttgatc ttgagcattc tgatgcatag aaggggtttt cctccgtgtt ctcaggacgc
     2581 aacgggtgca gccgcagacg gtacctcaat cagccggacc gcccggcgac cagggaattg
     2641 cttgttcaag agcggcaaag cgacgttgta gacgggagtg cctgtgaggg ttcttccttc
     2701 gaggctcccg ttgtgagcat gcccgtgaaa caccgccgtg acttcatagc ggttcaatgg
     2761 ttcctccaac cggctcgagc cgagaaacgc aaagatctcc ggaggctcgc cgcggaccgt
     2821 atcgacgaca ggcgcatagt gcatgagcac caccttggag gctgtccgca gcttggccag
     2881 cgcagattcc aacttcaccg tttcctccac ggattcccgc gcgaactgct tgagaaccgt
     2941 ctcaccccag ggttgcaagg cacgcgcgcc gaatcccccg caaaaccctt taataccggc
     3001 gaatcccact cccagaactt ctatcgcagt gccgtcgagg actgtcgccc cgccctccgt
     3061 aagaatagcg gtgatctcgt cgtgcttgcc gcattcgtac tcgtggtttc ccaggacggc
     3121 gatgattggc acgggtttga cctgcctgag gtgatcagcc agtactcgag cttcgtccgg
     3181 tttgccataa tcggtcagat ctccgcaaag gagcagcaca tccgcttcat tcgcgagatt
     3241 ttcgaacagt tcgcgcaact catcggccga cgtccgggga caatggaggt ccccgacggc
     3301 agcgacccgt atgactcccg tttctttgac gtccctgtta tccatcactt ccccatttca
     3361 cgtggacacg aaatctccgc cgaaacgcgg cagatcatgg cggcgcaagt tttccgcgaa
     3421 aagatcccga tcgatggctt gagcggccgt cgctccctgt cccgccgcga tgatcagttg
     3481 gcagttagcc ggagtcacac agccggccgc atacagaccg gagatagtgg tccgcatatc
     3541 cgcatcgacg atcaactgcc cttcctcgtc ttccgccgct ccgagtcctt cggctagtcc
     3601 cgtatgaaac acatcgcccc gggtggtgaa catcgcctga acctcgcacg atggtccctc
     3661 ttcgaacacc acggcggcga gtcgcccgtc ccggtgagac agttccttga tgcgctccgc
     3721 ccgtacaggg acaccgtatt ctgctaatgc agcaccccac gccacatccc acaccggcct
     3781 tctgccatcc gtcacgatgg tgacgaaggg cgaaaaggcc aacatggcaa gcgcatagcg
     3841 cgccgcctca ttgcgatgcc cgtaaatggc gatgcgcttc ccctgcacgc gataggcgtc
     3901 gcagtctttg caaaagaaga ccgtccgccc cagacaagcc tgcgcgccct ggatctcggg
     3961 cagcaaatga gtcaagccgg tcgcgagcag cacacgtctg ctccgatagg tcgcaggccc
     4021 agacgcgagc acgaacctgt cgctctgccg ggtgaggcct tcgacccggc cttcaactac
     4081 cgggacaccg aagcgccgca cctgtgcccg accccgctcc agcaaggact gcccggtgat
     4141 ggcttcggga aagcccaaat agttctcgac ctcgggctcc catttcgcca tgctttcgcc
     4201 gaggtcgaag agcaaaactc gacggcggct ccgacccaga tacaccgctg ccgaaagccc
     4261 ggcggggccg ccccccacga tcaccgcatc ccaaagctct acaccgctta gcgctgcggc
     4321 cggggagtac gtcacctccg tgttcaaggt cgggacggaa cggttggcga cttcagccgc
     4381 gatgggagcc ctctcggccc tcgcgaatgg attctttgcc gctttgcacg tactctcgtg
     4441 ccgagcgccc ggcgtcctgg accgcttcct gacccttctc gtagtattcc tttccccgct
     4501 caacggcact gtcccaggca gtccgtccac gttcccaggc ttcatccttg gctcgcgctg
     4561 cgtagttccg cagcatgcct ctcagttcag ttccagtttg gggtgcgaag agcaacgcca
     4621 gtccagcccc aatgaaggcg ccggccacga aagctgacca gcccatggcg tcatcttcat
     4681 aatattcgta atcaccattc cgcatcgttt gcctcctttg ttatgtgggt tcttgaaatg
     4741 tgggttatcg gtctgctctg cgcattcctt tcccgtcgga atgaagtgca atccgcggac
     4801 cgcctcgacg ccaggacgct gggcctgatt tcaagaggtt taagcgtccc attcgatcca
     4861 gcaggttggg ggaacgcgcc ggcgcgacag catgtccgtc aggtcggaca gtatgtccga
     4921 gctctaattg tgactcgtga gtgcggaatg agaatcgttt ccaaattttt acatcatatt
     4981 tacgctacgc tttcttccaa cgattgcacg tccgagaacc ggcgactact atgcaccgtc
     5041 ctttgattgt gaacccactt ctcgatgcca tcaaaacgga tgtatccagc agacaatcgc
     5101 cgctcttccg tgtatctctt gcaacgggca tggcctttca tcgcgccatt tcgcggacaa
     5161 ctgacttgcc tgtgtttcgt cgtcctcctg tccattcctc tcagcctgtt gacacccctg
     5221 cccctcacct tagctgttga cagcgtcctc gggactaagc ctcttccggt ctggctgcaa
     5281 gcctggcttc cacccaccgt ctcttcatcg ccggggagct tgctgttttt cgtatgcgcg
     5341 gcctacatcg gcgtcgccct gctgattcac gtacagagca tggtgctgtg gcttttgtca
     5401 tcctacaccg gggaacgctt gatctatgct tttcgtcagc gattgtttga acatcttcag
     5461 cgcatctgcg cctcttatca tgaggcacat ggacctaccg actcggtcta tcgcatccag
     5521 catgacgcgg cctcagtcaa acaaatcccg attgatgcgt ttttgccgtt cgtcaaggcc
     5581 tgctgcctct tgagcggact ggcggcgtta atgttgctga tcgatgcgga agtcgccgcg
     5641 gtcgctttga cgatgctgcc ggtgcttttt tggctcaccc accgctgcgg ccgccggttg
     5701 cggcaacaat ggaccgacgt caagtcgacg gagacagcga cagtggcgtg cgcccaggag
     5761 gtcttgagcg cttcgcgtct ggtcaaagcg ttcggacgag aagaacatga gcagcgacgg
     5821 tttctggctc acgccatgaa ctgggttcgg caacacaatg ctctcgcgtc catctgcagc
     5881 gggtttgatt tcttattcgg catgacgatc acaatcggaa ccgccatcgc gctgttcatt
     5941 gggatcagtc acgtgaaggg cgggcgtttg agcttgggcg atctcttgct gctgatggcc
     6001 tatatggcgc aactggccgg cccgttggac acagcggcca aaaaattgac tgaattacag
     6061 tcgcatttgg ttggctttcg gcgggcgctc gccattctcg acacgccgcc tctcgtcaca
     6121 gaccggcctg cctccacacc cttgaacaag gccaaagggc acgtcgcgtt ccgcggcgtg
     6181 tctttcgcct attcctcttc agccgccgtg ctcagggacg tgtcctttca ccttcccgcc
     6241 ggcacgagag tgggaattgt ggggccgagt ggcgccggca aatctacgat cgtcaatctt
     6301 ctcactcgat tccatgatcc gacctccggc gccgtgctcc ttgacggcac cgacctgcga
     6361 gagtaccggt tgaccgacct gcgcaagcag tttgcgatcg tccctcagga tccgcaattg
     6421 ttttccacga cgatcgccga gaacatccgt tacggcgacc tcctcgcaac gaatgcacag
     6481 gtcgaggccg cagccgaagc cgcccatgcc gccggattta ttcggtcatt gccagacggc
     6541 tatgaaacgc ccgtcggcga gcgtggctct cgactctccg gaggcgaacg ccagcggatc
     6601 gcgctggcgc gggcgttctt acgcgatgcg cccatcgtga ttcttgacga gccgacgagc
     6661 gcgctggatg cggggactga gaaggatttg atcgaggtga tggaacggct gaccagcggg
     6721 cgcacgacac tgatgatcgc ccatcggctg aacacgttgc gtcactgcga ctttcagctc
     6781 gtcctgcagc agggacgcgt cattgttcag gcgcctgatt gggcactcca gtcaccgtcc
     6841 ggagacttcg tgtcggtcta ggactgttct gtggaaggcg gatctgactg tctctgggct
     6901 gagccagtca cctggcgctc cgcttccagc cagtgttcca gagcgcgccc ctcctcgaat
     6961 ccgctggctt catacagcca gtacgcgcgt tgttggattc gcgcgatgac gtgcggatct
     7021 tgttcactcg acgtctcgtc ccgacgtgaa tccgttgacg aactcctgcc cggttcctgc
     7081 cgttctcgag acgccgtttc ggacggcgct tccgacttgc tgcgcttgaa tacggctttg
     7141 gcttttccgc tcttgcccct tgtctgcgtc atgctctctg gttcagtcat tcttgccccc
     7201 agtttccctt cacccatcac agagttcgtc agattcgcaa ggtgagacct ccttaaaggt
     7261 ctcaccttgg gatctgtgct tgaggttatt gttcctggcc ctttcctgca atagctgacc
     7321 ctgcctgatc tgtcaaggcc ttgatcgtgg taatcgttcc catgtccgac gcttccactc
     7381 tgaccatgtc gccttgctta aattgacatc cgctggagcc cccgactgaa aagccggaac
     7441 cacgagcgat gtccttgttg gccgcctcat cggtcgatcc gacaacgtcc ttcaactgtg
     7501 atggatcctt ggacggcgcg ggcatttgat gctcagtttg ttgctggttg ggatcgttca
     7561 acgcctgcat ctgctgctga gcttttgagg cgtcatggct ttgcatctgt ccttcggtcg
     7621 caggggaaat tggaatttcg tgcccttctt tcggatcctg cttgccggtg gacatcttgg
     7681 caccctctcc gcccgcacac accatgttgg tgtctttcgt gagacgaagt ttcactgatt
     7741 gtccatgatc gccttggatg gaatactcct cgccttgcac ctgttcgatt ttaccgataa
     7801 tgatttcagg gccacccaac agcacatttg acgagccccc tccttccacc ccaatcccct
     7861 gttggctctt ctgttgagat gtttctccct cgccaacccc tttgccggcc gattgttgct
     7921 cggcgaatgt agaccccgca cacgctccca ccaacagcat gctcaacacg cctcgcccta
     7981 tcccttgtgt ccagttcatc ggcaccctcc tttcagtagt agccattgtg gtttctttgc
     8041 cgggtcgctc cgtcactcca tgcggccctt acggcaaagg ctcgcaaaag acgggcctta
     8101 tcaagaagca atggaagcgt gaataatctg agaaaatact gccgtcagtc cctgcacgct
     8161 tggtcagatt gtcttccatg cgacatcgcg tcccaccatg ttgattccac caatgataag
     8221 acgcaacaat gaggaccaac gactcgggag gcccccagta tagaaagcct acacgtcatg
     8281 ggatctttag gtacgttcag tgtggttccg cttgacttgg atggagaccc gaggcaattg
     8341 cggatgtggt actcatcatg gtacggtgag gccgacctct gtggggccac atgtttatgg
     8401 tgcgttcctc gacaggtatt ttgcttctcc tctttctttc ggtttgcgcc gttcaaacca
     8461 tgggagcggc gtgccccgat gattgccttc gttactggtc ttccgccccc catcacgcgc
     8521 atgaacaaca tccgggcgct caggacagcc agacagaacc ggatggcagt gaaggggaaa
     8581 gcgcgttgga ggtggccctg tctcagggag acggcttacc cgttattcgc ctcacaacct
     8641 gtcgtgttgt ggaaccgtca ttgcgtccgc gaagcgcgtt tccgtcggac ttgtttcgcc
     8701 ctccgacggg catctgaccg cacactccga ttccatttcc accatgctga ggcgccgctg
     8761 ttcctgtctg gaacggaggg aggcttgcta tgagacgttg gtttattgtg ccgttctccg
     8821 ctttgctcat cgtcgtattc aacgcaggcg catgggcgga cgtatcgctt cccacatacg
     8881 ctctcgccga tatcatcggc attgcggtcg agcgcaatcc gctcgttgcc ggcgcccaag
     8941 cggcaatcga tcagagcacc ggccagcgga cggctgccgg cgcctacccg aatccgaccc
     9001 tgcatggcga tggcggaaag ggctacttgc gcgacgcggg tcccttgaca aatgaaattc
     9061 cgagatcagc aaacgaatac atggccgggt tcagtcagcc gattgaatgg ccggccaaac
     9121 gggctgcccg gcagcgagcg gcagaggcgg gactggccgg cgcatcggtt ggcctcgtcg
     9181 agacccgctt aaatttgacc gccgacgtga aagtggcctt ctatgacctt cttttggccc
     9241 aacgcgattt ggaccttgca aaaaacaacc tggctatcgt ggaggacgtg cgtcgaatcg
     9301 tcggcgtgcg tgtgaggctg ggtgaagcgc cgcaatttga cctcatcaaa gcagaagtgg
     9361 aagtccttaa ggccaatcag gccgtgacga gggcagaaaa caccgtccgt gtgaaccgcg
     9421 tcatcctgga caccttcacg gcgggcgcgc tcggtcctac ctatgccgtt tctggagatt
     9481 ttttgatgtt tccggccggc ctcgatctcg ggcagctcgc cactcgatcg attcgcgagc
     9541 acccttccat ccagcgtctc acccgctcga ttgaccaggc agaccggacc gtggaattcg
     9601 agcgtcaatc acgggtaccc aacatgaatt tgaacacgag ctatacacgc gaatacggcc
     9661 gtgaaggctt cgatgtaggc ctcagcgttc cggcgcccct ctggtatcag cgacagggcg
     9721 aaatcgccgg ctcgctgggc gcaaagcgac gggaagaggc cgaactcctt cgcacccgta
     9781 atgaactgct tagacaggtg aaccaatact ttcaggatgc acagacaacc gccaagctga
     9841 ttgaagtctt ggaaaagggg ttgttaaagc aggcggaaga agccctgcgc atcgccaaat
     9901 tcagttttca acagggcgcc gcgagtctac tggaagtctt ggacgcccag cgggtgcagc
     9961 ggcaaaccct ctttgactat gcgcaggcac ggttcgaact gtccgtagcc ttggcgagat
    10021 tggaacgtgc agtgggaggc cttctctaaa atcgatgtgg gtgtggaccg ctgagtcacc
    10081 cgctcggcgg ccggcactcg gagcctagaa cgcaggaatc atatgttcga cttgatctca
    10141 cgcctcacac agtccggctg cctgctcatg gtgttgtgca caataggctg caacagcgcg
    10201 gcagaggaat cgcccggaca acactccact cccaaagctc atgaaaccgg catcgtcacg
    10261 ttgaccgctg aagaaatacg aagcggagcg gtcgtcgttg agccggctgc ccgtggcgag
    10321 ttcaagctgc accgagattt ccccgcgacg gtcgtgccgg accatcatgc caccgcggat
    10381 atcaccgcgc tcgtccgggg tcgcgtcgtg gacgtctacg cggatttggg gcaacaggtc
    10441 aagggcggcg atctcctcgc catgatgtac agcagcgatc tggggatggc gcagtcggcc
    10501 tatctcaaag ccatcgccaa actctacgtg gccgaacgcg cgtacgagcg cgcgaccatg
    10561 ctcttacgag aaaaagtgat cggcctggcc gaatctcaac gccgtcaggg agaaatgctc
    10621 agcttacgag cggaaaaacg tgaaagtgaa gatcggttgc gcctcttggg catgagcgaa
    10681 gagcaaatcg ccaaattgaa caaagagcag aagatcctct cgtatgtgcc gattaccgcg
    10741 ccattcgatg gtcgggtgat cgcacgcaac ctcacgaagg gagaagtcgt cgaagtcacc
    10801 gaaaaactgt ttacggtcgc cgatctttct gaggtgtggg tgctcgcgaa tatcccggag
    10861 aaggacatca cgtttatccg cgcggacggc gaaagcgaga ggtcaggagt agagcagctc
    10921 gtagaagtcc tggtgacggc atatcctggt gaagtcttcc acggaaaagt cacgtacgtc
    10981 ggcgacgtcc tggatgtggc cacacgcacg atgaacctgc gcctggaatt gcccaaccca
    11041 caacgtaagt tgaagccgga aatgtatgcc acgatccgtg tctactcgtt accggagcca
    11101 ggggtgctga tcgtgcccga ggcggcggtg caacgcgaac gggaccgacg gtttgtcttc
    11161 gtccaacgcg acgcccatac cttcgaagca cgcgacgtca agctgggcga cggcaacggc
    11221 gacgtcgtca aggtgctcga cgggctccaa gagggcgaac cggttgtcgt caaaggggcc
    11281 tatgtgctga aatccgaact gctttccgat caaatctaga gagtccgaaa ccgcttcatg
    11341 cgagataccg tgttcaacgc ccacgctcct tgtatgtttt ccaccggcct aattacccat
    11401 gtcggcgttt cttgaattag ctcttcgcca gcgggtgttg gtccttgctc tggcctgcct
    11461 catggccgcc ggcggcatct attcatttcg aacgatcgcg atagacgcct ttccggacgt
    11521 caccacggtc ctcgtccaag tcgtgacgga ggcacaagga ctgtcgcccg ccgaaatcga
    11581 gcgcttcgtc acatttccac tggagcttca attgatgggg gccccaggac tgaccgatat
    11641 ccggtcgctg tccaaggtcg gcctttccat gatcaccgtc gtgtttcgcg acgatgtgga
    11701 tatttatttg gcacgccagg tcgtgttgga gcgtgtattg gaggttcagg atagcttacc
    11761 ccccggcgcc agatcggaac tggtgcccaa ttctccgggt ctcggcgaag tgtatcaatt
    11821 ctatttggag ggtcctcacg acaacgatgc ggactacaag ctcaccgaga ccgagctcat
    11881 ggagcggcgg acacttgaag actggatcat ccgtcccctc ctcaagggca cgccggatgt
    11941 ggtcgacgtc aactcgatcg gggggttcgt caagcaatac caggtcatgg tggaacccgg
    12001 tctgctgcgc aaatacgacc tcgcgctcca cgacgtcttt atggccgtgg caaacaacaa
    12061 tgccaacgcc ggcggcaaca tcctggaaaa aggctcagaa aaatacattg tcaggggcat
    12121 cggcctgatc aagacactgc aggacatcga aaacatcgtc gtgaaggagg cgcgtggcac
    12181 gccggtgtat gtcagagacg tggccgaagt gcagatcggc catgcggtac gacacggggc
    12241 ggcagtcgtg aacgggaagc gcgaagtggt cgcaggcatc gtcttgctgt tgcgcggggg
    12301 caatgcccgc gaagttgtgg aagccgtcaa ggagaagatc gatgagattc acgagcagaa
    12361 gctccttcca ggcggccttc ggatcgtgcc cttctatgac cgccttgaat tgatcgtcgc
    12421 cgcgcttcac acggtgtaca aggccctgct cgaaggcatc gtgctcgtca ttctggtgtt
    12481 gtttctcttc ctcggcaacg tcaggagcgc gttgatcgtg acggcaacgc tggtggtggc
    12541 gcccctggcc acgttcatcg tcatggatca ggtgggcctg acggccaatc tcatgtcgct
    12601 gggagggctt gtcattgcta tcggcatgat agtcgacggg tcggtcgtcg tggttgaaaa
    12661 tgtctatcgc catctgagcg aacatgcgcc cgtctcgtcc accagccgcg gtgtcatcgt
    12721 gctgaaggcg acgaaagaag tcgcgcaacc ggtcattttc ggcattctca ttattattct
    12781 cgtgtttctg ccgatcctgt ccctgcaggg catggaaggg aagatgttca agccgctcgc
    12841 ctacacgatt atgattgcgc tcctggtgtc actcctgctg tcgcttaccc tgtcgccggc
    12901 gctctgttcg ttggcgctca ccacggccac ggaggaagac acctggctgc tgcggcaggc
    12961 caagcgttgg tactcaccgg ccctgacctg gtcgctggcc catcgccgca tgatcctcac
    13021 ctccgcggtg accgtgctgg gtctgagtct catgctcttt ccgtttctcg gcggcgaatt
    13081 cattccgata ctcaacgagg cggccattac accccagacc atccggctac ccagtatttc
    13141 gttggagaaa tcaattgaaa ttgagctcga aatgcagcgc gcggtgatgg aattccctga
    13201 agtccgcatg gtggtctcga aaatcggtcg ctcggaactg ggcaacgatc cgcaggagcc
    13261 caatgccagc gatccggtcg tttcgctcaa gccgatggac gaatggacga cggccaagac
    13321 caagccggaa ttggacgatg cggttcgccg gcgcattgag cgtgtgccgg gagcgaattt
    13381 tttgctcagt cagccgattc agcagcgggt ggatgaactg ctctcgggtg tgcgctcgga
    13441 agccacggtc aagatcatcg gagaagacct cgaacaactt cggaagatgg ctgaacaggt
    13501 tcaggccatc atggcgggaa tcagcggcgt caaagacgta cgcgtcgagc aactgttcgg
    13561 acaggcctac ctctcaatcg atatcgaccg ggggaagatc gccagatacg gtatcaacgt
    13621 cgcgcacatc agggaaatca tttctacggc catcggcgcg gaggccgcga cacgtgttta
    13681 cgaaggccag aaacgattcg acctcattct ccgctatccc aagaagtatc gggacagcgt
    13741 ggagacgatc agcaacatcc tgctgagagc gtccacgggg gccctgattc ccttgggcga
    13801 gctggccaag gtcgaactcc gcgagggccc ggcgctgatc agcagggaag ggcttcagcg
    13861 cagaatctac gtcggcttca atacccttgg ccgcgacatt gaaagtatcg ttggggaagc
    13921 tcaggccaaa attgccaaac aggtgcaatt gccgacgggt tatcgtttga tctggggcgg
    13981 atcgttcgaa aatatgcaga gagccatggc ccgtttgaag atcattgtgc cgattacgat
    14041 cggactgatt tttttgctgc tgtttgcatc gtttaattcc gctcgtcatg cagcattgat
    14101 tatcatgaat ctcccgttcg ccttaatcgg aggaatagta gcgctgtggg tcaccggtga
    14161 atacctcagc gtgccggcat ctgtcggatt catcaatctc ttcggagtcg ctgtgctcaa
    14221 tggcatcgta ctcgtgtcct acatcaacca gctgcgagcc gaaggattgc cggaccacga
    14281 ggcgatcgtg cgcggatgcc tgctt
//
LOCUS       DASSCQ010000181        17125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_319762, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000181 DASSCQ010000000
VERSION     DASSCQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 17125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17125
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319762"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..184)
                     /locus_tag="VFH05_11920"
     tRNA            complement(109..184)
                     /locus_tag="VFH05_11920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(149..151),aa:Arg,seq:acg)
     gene            431..697
                     /locus_tag="VFH05_11925"
     CDS             431..697
                     /locus_tag="VFH05_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678973.1"
                     /translation="MPSLHVVADDEVLLAKTSTRAFPVKLIVQWCVGIFAAFVLGTWL
                     VWWSAVVQRTFTTEDIRSACQRVMPESVERCIDTVTIQRGGARR"
     gene            694..1713
                     /locus_tag="VFH05_11930"
     CDS             694..1713
                     /locus_tag="VFH05_11930"
                     /inference="COORDINATES: protein motif:HMM:NF036558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678974.1"
                     /translation="MMLRSRLAAGLFAVLSLIGGATHLYAQDGASERRPRVEIGLGGW
                     VITQGETKWAHNASTIAGLGNPTSKLTYKDTGTNIIEGTVRFWVTPSWFGRLTAGYGT
                     VGGGRLTDDDYLSVDGGAPSSRTFSDIKGDSTWYLNADVGRRVVEFPRSRGWLDVFAG
                     YQYWYQRYSANGLGQASCSTAGQTLDLDPGSPGSQTLCSPNTTVSSSIEAITNTTQWH
                     SIRVGASTEYRFTRRLSVLGTVALIPVSIVDNTDVHHLRPDLKQNPSISMVGYGIGAD
                     VDVGARYMIFRNFYANVGYRLWWNRTVDGTVTFHNAIGGSDEFPLTELQSFRHGVTFG
                     ISYAF"
     gene            complement(1821..2885)
                     /locus_tag="VFH05_11935"
     CDS             complement(1821..2885)
                     /locus_tag="VFH05_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6678975.1"
                     /translation="MTTDELERLLAQQPLGVLHRLARGRVHRHFRAGKRRLIELLLRH
                     SAENRAGFESDLLALISEREARPAIEPVRHQRSERPKTSQPGHRLHSAHHEPDHHEPA
                     ASLAAWLEGIGVPLPQPFVPDPWQEDSLAKLQSGDVIVSVPTGSGKTYVAVEAARRAM
                     DENRTVIYTSPLKALSNTKYTEFCRIFGADRVGILTGDRRDNGQAPLLIMTTEILRNL
                     LYDAAGGEIDLRLDTLGLVILDESQYLADPERGVVWEETIIFCPTQARLLLLSASIGN
                     PHDIADWLTSIRSTPCHLIKHSKRTVPLRAGYLHPDGKLTPLFRTIGIPSGHPVHLHP
                     EARRLFARYEDETMPSGRRR"
     gene            complement(2968..4824)
                     /locus_tag="VFH05_11940"
     CDS             complement(2968..4824)
                     /locus_tag="VFH05_11940"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET6678976.1"
                     /translation="MPPSIHPVDLIIALRQKHLTPAIVFLTSRRACDEAMESFEHTQS
                     FLPPARQEMIGKVLRAVIDQYPSIAEHPLIPTVQRIGVAAHHAGHLPSWKIAVEELMR
                     SGCLDAVFATTTLAAGVDFPARTVVITQSSIRKARDFTDLTIGEVQQIAGRAGRRGKD
                     LVGFAIVTPSPYIDLTVLTKGLTGHPEAINSQFVISYPMVLNLLKAHPLDQIQPILAK
                     SFAQFQLNQRAEVLERKLDELHRQLEPYGPRVCTDWISQWQLFDHVRRQRTHRTPVRR
                     NEPPEIAARFHFLTPGRVAGLSKSRGVVLRQYRSKGQKSPMVTLLRHGGAITECPAAI
                     VTEVFDHMLDCAEAPVYPWCTPESIESMLRDLEELPPRLPVLPILVPKDELIPDSIVQ
                     TLGDFSCPTCPSRSACQKDHAHASKLRQEQHRHTKAIQALRTSLWHRFQEKIDALQRF
                     GYLSPTAHLTDTGEWARLIRIDHSLLITELVRADAFTGASPALLAAVMSSIAHDDDRP
                     GAFPRISTGLASLLAQVRKLAESLSPYEDPPLLRADIAAITEQWIGDPTLTWIGLCKK
                     TTMAEGDIYRLLARTLEFLSQLHTLRDTHPALADCASQAIAILRRGVLEELP"
     gene            complement(4799..6037)
                     /gene="glgC"
                     /locus_tag="VFH05_11945"
     CDS             complement(4799..6037)
                     /gene="glgC"
                     /locus_tag="VFH05_11945"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET6678977.1"
                     /translation="MQNLFTMVLAGGRGERLHPLTEHRAKPAVPFGGKYRIIDFTLSN
                     CLNSGLRKVAVLIQYKSHSLDRHIRTGWDIFNAELGEYIASIPPQQRISEEWYRGTAD
                     AVYQNLFLLEADQPEYVLILAGDHIYKMNYMEMFHWLIAKGADVVVGALDIPIEESKR
                     FGIMSVDEDFSVKEFNEKPAKANHIPGDPSKAFASMGIYLFRAKSLREELIADAQENT
                     AHDFGRNIIPRMIGSRRVYAYNFQDANKKAVKYWRDIGTLDAYWEANLDLVAVDPLFN
                     LYDQQWPIRTYQGQFPPAKFVFAQDYQGGRMGVALDSIVCGGCIISGGRVQTSVLSPN
                     VRVQDHADVRESVIMENVVIGEHCRIKRAIIDKDVDIPPKTEIGYDRAADAQRFHVTD
                     SGIVVISKGMKLNASLDSSG"
     gene            6165..6458
                     /locus_tag="VFH05_11950"
     CDS             6165..6458
                     /locus_tag="VFH05_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678978.1"
                     /translation="MKEDVLVSSLAKKTAVLAKRKAAPEDQDTLRWRLEHLESQMQKL
                     SQLVRDHAEDVDRLVRIVAEDRNAIRQQLLRKHHQQVRVRKHLRASDSKPADY"
     gene            6637..7953
                     /locus_tag="VFH05_11955"
     CDS             6637..7953
                     /locus_tag="VFH05_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET6678979.1"
                     /translation="MFPQHTRCGVITISSFLCIISISLPQAWPAAPSVHQAAEPRPTP
                     SARLSLNDALALFLQQNLDVLIAKYGIEYSKGQQITARLFPNPVMSIGTLSSYTQGRT
                     LSTGGQLFFQAQQLFELAGKRGYRIESAGFGAQSAEAAFEDAVRQLGFTVKDSYYRVQ
                     LAQRRLALAEENRDRFSRILEINTIRFKKGYIAEVDLIRIRLQMVDFQSQVIQSLQEA
                     ESARGDLRQLLRLSPAMTVELSTDLEFRRLDPEIAKLRVAAIDVRPDLRSKRFTYSQR
                     EADLRLAQAYRVPDVTVGAGYAIQGSLGPDNPQQWALNLGLPVPLFNRNQGGIRQAEV
                     AVQTAEADLNKAVNVVENEVEVAYRNLLQSRRLVEAYVGGVLEDARATFTIVERAYER
                     GGATILDLLDAARTSRTIQQNYIEALFNYQRNVLQLESAVGQEIAS"
     gene            7950..9173
                     /locus_tag="VFH05_11960"
     CDS             7950..9173
                     /locus_tag="VFH05_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6678980.1"
                     /translation="MTAHHRIQPEVARDQPLDEARVRLLALRCAHVIWVLAITALVGC
                     GKGDQPANSDAATPPHTKSGSGPHEAVSRVETGIVDYSGSQQDLALSGKMAYGEDRYS
                     KISSPLQGRVVEVRAHLGDRVKAGAILLVVDSPDIAQAYSEFVKEDSDLQYATRSHEL
                     AKDLYANKALPLKDLKQAENELVKARAEFRRAKERLLSLRIPAEEVNKPLDKQQITSR
                     FEMKSPLTGIVVERMVTPGQSVGGDANQVLFTVADLDMLQVVADVYERDLALVKEGQF
                     AKVKVEAYPDIDFPATVASIGDVVDPASRTIKLRAWVNNPDHRLKPEMFARLHIQVGD
                     STRILVVPKESVLESDGKQFVFVVEEANRYIKREVKVANFTPDQMRVLDGLTPGQRIV
                     TKGAVLIKGQEVKGG"
     gene            9222..12290
                     /locus_tag="VFH05_11965"
     CDS             9222..12290
                     /locus_tag="VFH05_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET6678981.1"
                     /translation="MIARLVEISLVQRILLCALGLTLLFGGLYSFQLLDIIAYPDPSP
                     PMVELITQNPGWSAEEIERQITIPMEVALTGMPGLTDIRSLSIFGLSDIKVYFDFGTD
                     IFRDRQEVLNRLAAVDFPLGVQPVLSPWWAIAEIYRYELTGDPGINLTELKTIQDWQV
                     RRAFRRIPGVIDVTAFGGTTKEFHVDIDPGKLISYGVSLSQVMNALQNSNANVGGNYL
                     TLGAQNYNVRGVGLINSIEDIENVMVAQKDGTPIYVKTLGQVKVGSRIRLGKVGIDER
                     DDVVEGVVLLQRGYKALPVLDKVREKMDELNEWKLPAGVKIRTFYDRTTLIHTTVETV
                     TDIMISGMVLVFLLLYAFLGNLRAAGIVALTIPLSLLFTFGMMVLVGQSANLISLGSI
                     DFGIIVDATLIMVESIFFHLAHAKTPGLTVHQQIVRAARQVGQPIFYSTAIIVVAFIP
                     LFTMTGVPGKIFAPMSITYGFALLGALLMAFTLAPVLCSFLLTGPITEHDTMVVKPLR
                     KLYQRTLDWALDHRKMVVALAASLLIVALVALQFLGGEFMPALEEGNLWVRATMPVDI
                     SFDQAARLTSDIRAMFRESPEVTTVVSQLGRPDDGTDPTSFFNAEFLANLKPQAHWRR
                     GLGKDDLVEEIESRLKTIPGVIFNFSQVIQDNVEEAMSGVKGENSIKLFGADLKTMET
                     LAGDIEKAMHQVRGVKDLGIFRLLGQPNLLIKVDRQASARYGLQVADVNAVVQAAIGG
                     QAVTQVYEGERLFDLVVRFLPEYRQDVEAISNILVSTPDGARIPLKQVATITSQTGAF
                     IIYRENNERYIPIKFSVRDRDLESTVEEAQALLAKRITLPERYRMEWAGQYDQLKDEQ
                     KRLAKIVPLSLLLILFLLYITFDSLKNALLVLSAVPFALIGGVIALVATHTNFSISAA
                     VGIISTLGVAILGGVLLISRIEDGRMAGLNLREAIIQAAAVQMRPILMATIGAAIGLL
                     PAAVATGIGSQAQKPLARVVVGGMLTAAFLILVVLPVLYEMVHRRTDQDE"
     gene            12312..13103
                     /locus_tag="VFH05_11970"
     CDS             12312..13103
                     /locus_tag="VFH05_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6678982.1"
                     /translation="MRWKWMLVTVVMMGLGLSPGGPELFAQSGLRGLTVPVQIPYEMP
                     PQFQETAPAVSVPPNTRPLTPEELQRAESLLPMLEGKQEFWAMGEFVHLGEPSVPVLV
                     KALTMPGPRTRYNAIETLSMLNAASAVPALIASAKDPNEIPRVREHALRVAIRLDASK
                     TPEAIQAMATDQNSSIRKAAAFGSRYVRDKAVVPVLIGMIGDDERFVALSAVQSLWIL
                     TLHETEFHDWEASTKADRQEWMQEWTEWWSGQKETFQIPEPRRRS"
     gene            13233..13763
                     /gene="def"
                     /locus_tag="VFH05_11975"
     CDS             13233..13763
                     /gene="def"
                     /locus_tag="VFH05_11975"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6678983.1"
                     /translation="MAVLPIAKLGNPILRKIAAPVDPRDIRSREFQQLIDDMYETMLE
                     EPGIGLAAPQVSRSIQLVVMACEGEGGFPATTLINPTIVYYGPQQVENWEGCLSLDGL
                     RGKVTRPALVRVKALDRNGKPLDLEADGLYSVCIQHEMDHLIGKVFVDRMTDLSTLTQ
                     LNEFDKYWRKEPTPVI"
     gene            13785..15509
                     /locus_tag="VFH05_11980"
     CDS             13785..15509
                     /locus_tag="VFH05_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6678984.1"
                     /translation="MKTLLRVLQYLRPHRSMVIGTFLFAGLTTAFELVPPWLIKVLID
                     DVIQAGKTQLLSLVFGGLCTAYVLRSVCASMRIKLNNSLEQQIVHDLHVQVFGALQRL
                     SISFFENRPTGEIMSRVLNDTEHMQRIFVDGLEEIITAGLTLIGIMAVLFWLNWKLAL
                     LALVPIPLLVLGAIAFTKRIHKYYRKIRKGSAELNALLQDTLAGIRETMGFNRQPYEE
                     DRFGRKSDQCRKDTLKAMYLWSYYSPGMMLAGSLGAALVLWNGTHEVLGQRLSVGELV
                     MFMSYLALFYVPINQIHSVNHMLQHALAASERVFEVLDIVPEVKDEPGLRAPVPRLTG
                     EIAFRRVHFHYRPDAPVLYDVSLIVRAGERVALVGPSGAGKSTTLKLLTRFYDVTGGE
                     ITIDGHDLREMPLAFLRSQIGLVQQEPFLFNGTVRENILYGNLSASHDDVEAAATAAR
                     AHEFIVNLPEGYDTWIGERGVKLSVGQRQRVSIARVLLKDPPIVMFDEATSNIDTETE
                     VKIREALEELTKDLTKIVIANRLSTVHGMYRIIVLEHGRVVEEGPHEVLITRGGVYAG
                     LYEAQFQT"
     gene            15751..15960
                     /locus_tag="VFH05_11985"
     CDS             15751..15960
                     /locus_tag="VFH05_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678985.1"
                     /translation="MTRSAGRKTVAVMRGLMMLCDSCYDQVLAEHLTDHKNFVQNYEA
                     LFDTICPGCTDINRPLVDDMLGSSE"
     gene            complement(15985..16308)
                     /locus_tag="VFH05_11990"
     CDS             complement(15985..16308)
                     /locus_tag="VFH05_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678986.1"
                     /translation="MAYMRTILLAVAVLLIARPGLTEDKSYYSPVIQVDKDNHRILIS
                     TLGSVFYVDVPAAAQPHMEKLPISGLVDFVVEMRGDGQMPLVKTWKVKSGESTCMYFN
                     GKECK"
     gene            complement(16412..16894)
                     /locus_tag="VFH05_11995"
     CDS             complement(16412..16894)
                     /locus_tag="VFH05_11995"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET6678987.1"
                     /translation="MNDLVAHARQLDPYECCGFLAGTDKTVTRLYRIKNVVSLDGASE
                     AASFDEAKMNHLSQLSPEERAEIAFIMDAQEMFQAVKDMRKHGLTLQVVYHSHPHDPA
                     RPSVTDIKIANEWEENWSRLNLSVPIYMLVSLMDKARPDVRAYWIKNASVSSADVVTL
                     "
     gene            complement(16926..>17125)
                     /locus_tag="VFH05_12000"
     CDS             complement(16926..>17125)
                     /locus_tag="VFH05_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyltransferase"
                     /protein_id="HET6678988.1"
                     /translation="ILQASEAIKEILAIGETLADKLLIYDALEMKFRKVSRPKDPDCP
                     LCGPNPRIKDLSTDYTVSCSL"
BASE COUNT         3642 a         4716 c         5044 g         3723 t
ORIGIN      
        1 tgagtgacca tttacgtcca cgaagggcta aagcagtttc ttcacacggt tccagacggt
       61 tcccgcgcta gaaagaattt tgaggatgat tcgtaattgc ttgaaaaatg gcgcgcccga
      121 caggagttga acctgtaacc cccagatccg tagtctggtg ctctatccat tgagctacgg
      181 gcgccctgaa gaactgctgg agtgctgagt gagtgaatgc cgagatagag tgacgtgaat
      241 agaaaatctc aggctcaaca cgcggtctca gccctgctga agaggcagcc agcggttata
      301 caggtccaag ggccaaaggg tcaagggttc gcacgcgatc gcgtcgactc aagaaccaca
      361 aaataatgtt cagatcgatc gaactccagt aggataggga ccggtgtcct agacctgctg
      421 gagttgtact atgccaagtc tccatgtagt agctgatgac gaggtcctgc tcgccaagac
      481 ttccacacga gcgtttccgg tcaaactgat cgtgcaatgg tgcgtcggga tctttgccgc
      541 cttcgttttg ggaacttggc tggtctggtg gtctgccgtc gtgcagcgaa cgttcacgac
      601 cgaagatatc cggtcggcct gtcagcgggt gatgccggag tcagtcgaac gttgcattga
      661 tacggtgacc atccagcgag gaggcgcaag acgatgatgc tgcggtcgcg cctggccgca
      721 ggcctcttcg ctgtgctttc cctgataggg ggagcgactc atctctacgc gcaggatggt
      781 gccagtgagc ggcgtccgag ggtggagatc ggcctcggcg gctgggtcat cacccaggga
      841 gagaccaagt gggctcacaa tgcctcgacc attgccggtc tgggaaatcc gacctcgaaa
      901 ctcacttata aagacactgg aacgaacatc attgaaggca cggtccgttt ttgggtcacg
      961 ccatcatggt tcggtcgttt gactgccggc tatggcaccg tgggtggagg acgtctcacg
     1021 gacgatgatt atttgtccgt cgatggagga gcgccgtcct cccggacttt cagtgacatc
     1081 aagggcgaca gcacatggta tctgaacgct gacgtcggcc ggcgcgtcgt agaattcccc
     1141 cgatcgcgtg gctggctgga tgttttcgct ggatatcaat actggtatca gcgctatagc
     1201 gccaacggtc ttggccaggc gagttgttcg actgccggtc aaacgctgga tctcgatccg
     1261 ggcagcccgg gaagccaaac cctctgcagc cccaatacca ccgtcagtag ctccatagaa
     1321 gccattacca acacgactca gtggcactcc atccgggtag gagcatcgac tgagtatcgc
     1381 ttcacgcgac gactaagtgt cctggggacg gtggcattga tccctgtcag tattgtcgat
     1441 aacacagatg ttcaccatct gcggccggac ctgaagcaaa atccaagcat ctccatggtg
     1501 ggttatggca tcggggctga tgtggatgtg ggcgcccgtt acatgatttt cagaaacttc
     1561 tatgcgaatg tcggttatcg cctctggtgg aaccgcactg tcgacggaac cgtcaccttt
     1621 cacaatgcca tcggcggctc agacgagttt cccctcacgg agcttcaaag cttccggcac
     1681 ggcgtcacgt tcggcattag ctacgcattc taaaccaata accgcttgtc ctgactcaga
     1741 ggagtcacct tccccgtcgc gctccgttaa gagcctcacg tcctgcgaga tcccgtcggt
     1801 tggagattgg tcgtagctgc tcagcggcgc ctgccagacg gcatcgtttc gtcttcgtag
     1861 cgggcgaaga gtcgtcttgc ttcaggatgt aaatgcaccg gatggccgga gggtattccg
     1921 atggtgcgga agagtggcgt gagcttgcca tctgggtgca gatacccggc acgcaagggc
     1981 accgttcgct tactgtgctt gatcaagtga cagggcgtgg aacggatgga cgtgagccag
     2041 tccgcaatgt cgtgcggatt cccgattgag gcggagagga gcaacagtct cgcctgcgtc
     2101 ggacaaaaga tgatggtctc ttcccagacg actccacgtt ccgggtcggc cagatactgc
     2161 gattcatcca agatgaccaa tccgagcgta tcgagccgca ggtctatctc gccaccagct
     2221 gcgtcgtaaa gtaaattgcg cagaatctca gtggtcatga tcaagagggg agcctgcccg
     2281 ttatcgcgac gatcgcccgt caggatgccg acgcggtctg ctccaaagat gcggcaaaat
     2341 tcagtgtact tcgtattcga aagcgccttc agcggcgagg tataaatgac ggtgcggttt
     2401 tcgtccatgg cacgtcgtgc cgcttccacc gcaacatacg ttttcccgct gcctgtcggc
     2461 acgctgacga tcacgtctcc gctttgcagc ttggccagcg agtcctcctg ccacggatcc
     2521 ggaacgaacg gttgagggag aggcacgccg attccttcga gccaggcggc taacgacgcc
     2581 gcaggctcgt gatgatccgg ctcatggtgc gctgaatgaa gccgatgtcc gggctgcgat
     2641 gtcttggggc gttcggatcg ctggtgccga acaggttcga tcgcaggcct cgcttcacgc
     2701 tcactaatca gcgccagcaa gtccgattca aagcctgccc ggttttcggc cgaatgacgg
     2761 agcaagagct caatgagtcg gcgctttccc gcgcgaaaat gccgatgcac tctgccgcgg
     2821 gcaagacggt gcagaacgcc gagcggctgt tgggcaagga gacgttcgag ttcgtcggta
     2881 gtcatggctt tagtctaaag ttcaacacat cgaacactgg cagggcaccg cgcgccatgc
     2941 ctacgacttc gagtacaccc attcgcgtca cggcaattcc tccagaactc ctcggcgcaa
     3001 gatggcaatc gcttgggagg cgcagtccgc taatgccggg tgcgtgtccc tgagggtgtg
     3061 gagttgtgaa agaaactcga gcgtgcgtgc aagcaggcga taaatatcgc cctcggccat
     3121 cgtggtcttc ttgcagagac cgatccaggt gagggttgga tcgccgatcc attgctcggt
     3181 aatcgcggca atgtcggccc tgagaagggg aggatcttca tagggcgaaa gcgactcggc
     3241 cagttttcgc acttgtgcca gcagcgaagc gagcccggtg ctgatacggg ggaacgcccc
     3301 gggccggtcg tcgtcgtgcg caatgctcga catgacggca gccaggagag ccggagaagc
     3361 cccggtgaag gcgtcagccc ggacgagttc ggtaattaag agcgaatggt cgatgcgaat
     3421 gagcctggcc cattcaccgg tgtcggtcaa gtgggcggta ggcgacagat atccgaaacg
     3481 ttgcaacgca tcgatttttt cctgaaagcg atgccatagg ctggtccgga gcgcttgaat
     3541 cgcttttgta tgacggtgct gctcctgacg cagttttgaa gcgtgggcat gatccttctg
     3601 acaggccgag cgggacggac aggtcgggca ggagaaatcg cccaatgttt gcacgatgga
     3661 atccgggatc agctcgtctt tcggaaccag aatgggaagc accggcaggc gtggcggcag
     3721 ctcttcgaga tcgcgaagca tggattcgat gctttccggt gtgcaccagg ggtagaccgg
     3781 agcttcggcg caatccagca tgtggtcgaa gacctccgtg acgatcgcag caggacattc
     3841 ggtgatggcc cctccgtgtc gcaacagtgt gaccatggga cttttctgtc ccttgctccg
     3901 gtattgtcgg agcaccacac cccggctctt ggaaagtcct gcgacgcgcc cgggcgtcag
     3961 gaaatgaaaa cgtgcggcaa tctccggcgg ttcgttcctt ctgaccggcg tgcgatgagt
     4021 gcgttgccgg cgaacatgat cgaatagctg ccactgcgag atccagtcag tgcagacacg
     4081 ggggccgtaa ggctccaact gccggtgcaa ctcgtcgagc ttccgttcga gcacctcggc
     4141 tcgttggttc agttggaact gcgcgaagct cttggcaagg atcggctgaa tttgatcgag
     4201 cggatgggcc ttcaggagat tcaagaccat gggatagcta atgacaaact gactgttgat
     4261 cgcttccggg tggcccgtga gccctttggt aaggacggtc aggtcgatat aaggggacgg
     4321 cgtgacaatg gcaaagccga ccaagtcttt tcccctacgg ccggcccgtc cggcgatctg
     4381 ctggacctct ccgatagtca gatcggtgaa gtccctcgcc ttgcgaatgc tggattgcgt
     4441 gatcacgacc gtccgggcag gaaaatccac accggccgcc aacgtggtgg tggcgaagac
     4501 cgcatccaga cagccgctcc gcatcaattc ttcgacggca atcttccagg agggcagatg
     4561 cccggcatga tgtgcggcga caccgatccg ctggaccgtg ggaatcaaag gatgttcggc
     4621 aatgctcgga tattggtcga tgacggctct caggaccttt ccgatcatct cctgccgcgc
     4681 cggcggcaaa aaggactgcg tgtgttcgaa ggactccatc gcctcgtcgc aggcgcgtct
     4741 cgacgtcagg aacacgatgg cgggggtgag gtgtttctgt cgtagggcga tgatcagatc
     4801 aaccggatga atcgagggag gcattcagtt tcataccttt tgaaatgacc acgatcccgg
     4861 agtcggtcac atggaagcgt tgggcatcgg ccgcgcggtc gtagccgatc tcggtcttag
     4921 gcggtatgtc cacgtcctta tcgatgatgg cgcgcttgat ccggcagtgt tcaccgatca
     4981 ccacattctc cataatcacg gattctcgca cgtccgcgtg gtcctggaca cgcacgttag
     5041 gcgacaacac gctggtttgc actcgccctc ctgaaataat gcagcctccg cagacgattg
     5101 agtctaacgc cacccccatg cgccctccct ggtaatcctg ggcgaagaca aacttcgccg
     5161 gcggaaactg tccctggtag gtgcgaatgg gccattgctg gtcgtagagg ttgaataggg
     5221 ggtccaccgc cacgagatcg agattggcct cccagtaggc gtcgagcgtt ccgatatctc
     5281 tccagtattt gacggccttt ttattggcat cttgaaagtt ataggcgtac acgcggcggg
     5341 agccgatcat ccggggtatg atgttcctgc cgaaatcgtg cgccgtgttt tcctgtgcat
     5401 cggcaatcag ttcctcgcgc aaactcttgg cccggaagag atagatgccc atcgatgcga
     5461 aggcttttga cggatcaccg ggaatgtgat tcgctttcgc cggcttttca ttgaattcct
     5521 tgacgctgaa gtcttcatca acactcatga tgccgaatcg cttcgattcc tcgatcggaa
     5581 tatcgagggc gcccacgacg acgtcggcgc ctttagcaat gagccagtgg aacatttcca
     5641 tataattcat cttgtaaatg tgatcgccgg ccaaaatcag gacatattcc ggctgatccg
     5701 cctcgaggag gaacagattc tgatacaccg catcggcggt gccgcgatac cactcctcgc
     5761 tgatccgttg ttgcggggga atcgaggcga tgtattctcc cagctcggcg ttaaagatgt
     5821 cccatcccgt tcggatgtgg cgatcgagcg agtgcgactt gtattggatt aacaccgcga
     5881 ctttgcgaag gccggagtta agacaattgc ttaaagtaaa gtcgatgatg cggtattttc
     5941 cgccaaaggg cacggccggc ttggctcgat gctcggtgag cgggtgcaac cgttcacctc
     6001 gccctccggc gagcaccatc gtaaaaaggt tctgcattgc gccgattgta gcaggaaccc
     6061 tcagcaaata gaaagaaagc gaatccgttg acttccagca tggggcagtt gatactacga
     6121 tctcgaatgc gcgttcatcg tgatggcggg ctgaaggggt ggatatgaaa gaagacgtgc
     6181 tggtgtcgtc actcgcaaag aaaacagccg tgcttgcaaa gagaaaggcg gcgcccgagg
     6241 accaagatac cctccggtgg cgcttggaac atctcgaatc ccaaatgcag aagctctctc
     6301 aactcgttcg agaccatgcc gaggacgtcg accggttggt ccgcatcgtc gccgaggacc
     6361 gtaacgcaat ccgccagcag ttgcttcgta aacaccacca gcaggtcaga gtgcggaagc
     6421 acttgcgcgc atccgattcc aagccggctg actactagct ttccggcacc tcttccactt
     6481 cctgcgtcca ggctgccagt tcgactaagt gctgatttat tcagagaata tttgcatcat
     6541 atgtaggaca ctgtataatg cagctgtctt gtgccgacct tgaggtggct tccctgaacg
     6601 cctcgacgcg cagtggttgt ctgtgaggac ccgtacgtgt ttccgcaaca tacgcgatgc
     6661 ggagttatca cgatctcgtc gttcttgtgc atcatcagca tctcccttcc ccaggcgtgg
     6721 ccggctgcac cctctgtcca tcaggcagct gaaccgcgcc cgacgccgtc cgcacggctg
     6781 agcctgaatg acgcgctggc cttgttcctt caacaaaatc tcgatgtctt gattgcgaaa
     6841 tacggcattg aatacagcaa gggccaacaa atcacggcga ggctgtttcc caaccccgtc
     6901 atgtcgatcg gcacgctgag ttcctatacc cagggacgga ctctgtccac gggcggtcag
     6961 ctattctttc aagcccaaca gttgtttgaa ttagccggta aacgagggta tcgcatcgag
     7021 agtgccgggt tcggagcgca gtccgccgag gccgcgtttg aggatgcggt ccgccagttg
     7081 ggcttcacgg taaaagacag ttactaccgg gtgcagttgg cccagcggcg gctcgccctg
     7141 gctgaagaaa accgtgatcg gttttcccgc attctcgaga tcaacacgat ccggttcaag
     7201 aagggctata tcgctgaggt ggatttgatt cggatcaggt tgcagatggt tgacttccaa
     7261 tcccaagtga tccaatcctt gcaggaagcg gaatcggctc gcggcgattt gcgacagctc
     7321 ttgcgcctct cgccggcgat gaccgtcgag ttgtcgaccg atttggaatt ccgccgcctc
     7381 gatccggaaa ttgcgaaact gagggtcgcg gcgatcgacg tgcgtcccga cctgcgttcc
     7441 aagcgcttta cctactccca acgggaagcc gatctccggc ttgcccaggc ctaccgcgtc
     7501 cccgatgtga cggtaggagc cggctatgcg atccaaggct cgctcggtcc tgataatccg
     7561 caacaatggg cgctcaacct cggattgccc gtgccgctgt tcaatcgaaa ccagggcggg
     7621 atccgacagg cggaagtcgc ggtccaaacg gcggaagccg acttgaataa ggctgtcaac
     7681 gtggttgaga atgaagtgga agtcgcttac agaaatctcc tgcagagccg gcggctggtc
     7741 gaagcgtacg tggggggagt gttggaggat gcgcgcgcca ccttcacgat cgtggaacgg
     7801 gcctatgaac ggggcggtgc gaccattctc gatctgctcg atgccgcacg gacgtcgcgg
     7861 accattcaac aaaattatat cgaggcgttg ttcaattacc agcggaacgt ccttcagttg
     7921 gaatcggcgg tcggccaaga gattgcgtca tgactgcaca ccataggatt cagcctgagg
     7981 tcgcgaggga tcagcctctg gatgaggcgc gcgtccgact ccttgctctc aggtgcgcac
     8041 acgtcatctg ggtgctggcc atcaccgctc tcgttggctg cgggaaggga gatcagcccg
     8101 ccaactcgga cgccgccact cctccgcaca caaaatccgg cagcggcccc cacgaggcgg
     8161 tctcgcgcgt ggaaaccggc atcgtggact acagcggatc acagcaggat cttgccttat
     8221 ccggcaagat ggcttacggg gaagaccgct attcgaaaat ttcctcgccg ctgcagggtc
     8281 gggtagtcga ggtccgtgcc catctcggcg atcgggtcaa ggccggagcg attcttctcg
     8341 tggtcgacag tccggacatc gcgcaggcct actccgaatt tgtgaaggaa gactccgatc
     8401 tacagtatgc cacccgttcg cacgagctgg cgaaagatct gtacgcgaat aaggcgcttc
     8461 cgctcaagga tctgaagcag gctgaaaatg aattggtgaa ggccagggct gagttccgcc
     8521 gggcgaaaga acgcttgttg tccttgcgca tcccggccga agaagtcaac aagcctctcg
     8581 acaaacagca gatcacctcc cgctttgaga tgaagagccc tttgaccgga atcgtcgtcg
     8641 aacgcatggt gacgccggga cagtcggttg gaggggacgc caatcaggtg ctgtttaccg
     8701 ttgccgatct tgacatgctg caagtggtgg ctgatgtcta tgaacgcgat ctggccttgg
     8761 tcaaggaagg gcagtttgcc aaagtcaagg tcgaggcgta ccccgatatc gactttcccg
     8821 caaccgtggc gtcgattggt gacgtggtcg atccggcgag ccgcacgatc aagctccggg
     8881 cctgggtgaa caatccggac catcgtttga agccggaaat gttcgcccgg ttgcacattc
     8941 aggttggcga ctcaacgcgc atccttgtcg taccgaaaga atccgtcttg gagtccgacg
     9001 gcaaacaatt tgtcttcgtc gttgaggagg ctaaccgcta catcaagcgg gaggtcaagg
     9061 tcgcgaactt tacgccggac cagatgcggg tcctcgacgg tttgacgcct ggccagcgga
     9121 tcgtgaccaa gggcgccgta ttgattaaag gtcaagaagt caaaggcggc tgactgagtg
     9181 tctccttcct atcgctgtaa tcccccggat ccaatgagtt gatgatcgcg cgtctcgtcg
     9241 agatatcgct cgtccagcgt attttgcttt gcgccttagg attaacgctc ctgtttggcg
     9301 ggctctactc cttccaatta ctcgacatca tcgcctatcc ggatccctcg ccgccgatgg
     9361 tggaattaat cacgcagaat cccggatggt cggctgagga aatcgaacgg caaatcacga
     9421 ttccgatgga agtggcgctg accgggatgc ccggtcttac cgacatccgc tcactgtcca
     9481 tcttcggctt gagtgacatc aaggtctact tcgactttgg caccgatatc ttccgcgacc
     9541 gtcaagaagt gctcaaccgc cttgcggctg tcgactttcc tctcggcgtt caaccggtac
     9601 tttcaccctg gtgggccatc gcggaaattt atcggtacga gctcacggga gatccgggca
     9661 tcaacttgac cgaactcaag accattcagg attggcaggt gcggcgggca ttccgacgca
     9721 tccccggcgt cattgacgtc accgccttcg gcggcacgac gaaagaattc cacgtggaca
     9781 tcgatccggg aaaattgatc agctacgggg tcagcctgtc ccaagtcatg aatgccctac
     9841 agaacagtaa tgcaaacgtc ggaggcaact atctcacgtt gggagcccag aattacaacg
     9901 tgcgtggggt cggtctgatc aacagcattg aagacattga aaacgtgatg gtggcgcaaa
     9961 aggacggcac gccgatttat gtcaaaacgc tcggtcaggt caaagtgggc agccgaatcc
    10021 gactgggaaa agttggcatc gacgagcggg atgacgtcgt ggagggcgtc gtgctgctcc
    10081 aacgcgggta caaggcgctg ccggtgctcg acaaggtccg cgagaagatg gatgagctca
    10141 acgagtggaa attacctgcc ggtgtcaaga tccggacgtt ttatgatcga acgacattga
    10201 tccatacgac cgtcgaaaca gtcacagaca tcatgatcag cgggatggtg ttggtcttcc
    10261 tcttgctgta tgcgttcttg ggcaatctcc gggcagccgg cattgtcgcg ctcaccatcc
    10321 ccctgtcgct cctctttacg ttcgggatga tggtgcttgt gggccagtcg gcgaacctga
    10381 tttccctcgg ctccatcgat ttcggcatta tcgtggacgc gacgctcatc atggtcgaga
    10441 gcatcttctt tcatttagcg catgcgaaga ctccaggcct gaccgtgcac cagcaaatcg
    10501 tgcgggcggc acgccaggtc ggtcagccca ttttttattc gacggccatc attgtcgtgg
    10561 ccttcattcc gctgttcacg atgacggggg tgccgggaaa aatttttgct cccatgtcca
    10621 ttacctatgg attcgcctta ctcggcgcgc tgttgatggc ctttacgctg gccccggtgc
    10681 tgtgctcctt cctgttgacg ggaccgatta cggagcacga tacgatggtc gtcaagccac
    10741 tgagaaagct gtatcaacgc acgctcgact gggcgctcga tcaccggaaa atggtcgtgg
    10801 ccctggctgc ctctctcctc atcgtggccc tcgtggcgct gcagtttctt ggcggtgaat
    10861 tcatgcccgc gttggaagaa ggaaatctct gggtgcgcgc caccatgccg gtcgatatct
    10921 cattcgacca ggcggcgcgg ctcacgagcg acatcagggc gatgttccgg gagtctccgg
    10981 aagtgacgac ggtcgtctct cagctaggcc ggcccgatga cggtaccgac cccacgagtt
    11041 ttttcaacgc tgagttcctt gccaacctga agccgcaagc gcattggcgc cgagggctcg
    11101 gcaaggatga cctggttgaa gagatcgaga gccggctcaa gacgatccca ggggtcatct
    11161 tcaatttttc acaggtcatt caggacaacg tcgaagaggc gatgtccggc gtcaagggag
    11221 aaaactcgat caagttgttc ggggccgatt tgaaaacgat ggaaacgctg gccggcgaca
    11281 tcgaaaaggc catgcatcag gttcgaggtg tgaaggactt gggaattttc cgcctgctcg
    11341 gccagcccaa tttattgatc aaggtcgacc ggcaggcgag tgcccgctac gggttgcagg
    11401 tggccgacgt caacgccgtt gtgcaggcgg caatcggagg tcaagccgtg acgcaggtgt
    11461 acgaaggcga gcgcttgttt gatctcgtcg ttcggtttct ccctgaatat cggcaggacg
    11521 tcgaggccat cagcaacatt ttggtgagta cgcctgatgg cgcccgcatt cccttaaagc
    11581 aggtcgccac gatcaccagt caaaccggag cgttcattat ttatcgcgaa aacaacgagc
    11641 ggtacatccc cattaaattc agcgttcgag atcgggatct tgaaagtacc gtcgaggaag
    11701 cgcaggcgct gcttgccaag cgcattacgt tgcctgagcg atatcgcatg gaatgggccg
    11761 gccagtacga tcaattgaag gatgagcaaa agcgcttggc caaaatcgtg ccgctgagcc
    11821 tcctgttgat tctcttcctg ctctacatta ccttcgactc gctcaaaaat gccttgttgg
    11881 tcctttcggc ggtacccttc gcgttgatcg gaggcgtgat cgcgctggtc gccactcata
    11941 cgaacttcag catctcggcg gcggtgggta tcatttccac cttgggggtc gcgatcctgg
    12001 gcggcgtctt actgatctct cgcatcgaag acggccgtat ggcaggactg aacctccgcg
    12061 aagcgatcat ccaggccgct gccgtacaaa tgagaccgat tttaatggcg acgatcggcg
    12121 cggcgatcgg actgctgccc gcagccgtgg cgacgggtat cggctctcag gcccaaaagc
    12181 ccctagccag ggtggtcgtc ggtggaatgc tcacagccgc cttcttgatt ctggtagtgt
    12241 tgccggtgtt atatgaaatg gtgcatcggc gaacggacca agacgaataa cgaaagagga
    12301 cgatcgtgta tatgagatgg aaatggatgt tggtcacagt cgtcatgatg ggtttgggcc
    12361 tcagtccggg cggacctgag ttgtttgcac aatcaggcct gcgcggcctg accgttcccg
    12421 tgcaaattcc ctatgaaatg ccgccccagt ttcaggagac ggcccctgcg gtcagcgttc
    12481 ctcccaatac caggcccctg actccagagg agctccaacg tgcggaatcc ctgctgccga
    12541 tgctggaggg aaagcaagaa ttctgggcta tgggggagtt cgttcaccta ggggaaccga
    12601 gtgtacctgt tctggtcaaa gccttgacca tgccggggcc gaggactcgc tataatgcaa
    12661 ttgagacgct ctcgatgctg aatgcggcct ccgccgtccc tgcgttaatt gcgtccgcaa
    12721 aggacccgaa tgaaattccc cgggtaaggg agcatgcgct gcgcgtagcg atccgtctgg
    12781 atgcctcgaa gacgccggag gcgatccagg cgatggccac cgatcaaaat tcttcgatca
    12841 ggaaggcagc ggcgttcggt tcgcggtatg tccgtgacaa agcggtcgta ccggtgctga
    12901 tcgggatgat cggcgacgat gaacgctttg tcgccctgtc cgccgtccaa tcgctctgga
    12961 tcctcacgct acatgagact gaattccacg attgggaggc gtccacaaaa gccgatcgcc
    13021 aggaatggat gcaggaatgg acggaatggt ggtccggaca gaaggagacc tttcagattc
    13081 cagagcctcg gcggcgctcg tgatcatgca acagtgatga gacgctggaa agggcccatc
    13141 ctgcacagct ttccacatgt tgcggctcaa aaaaacactg tgttatacgt catgcagggc
    13201 cctcactcag aggccgtggc gaggcgaagg taatggctgt tctcccgatc gcaaaactgg
    13261 gaaatccgat cctgcgtaaa attgcggctc ctgtagaccc gcgcgacata cgcagcaggg
    13321 agttccagca actcatcgat gacatgtacg aaaccatgtt ggaagagccg ggcatcggtc
    13381 tcgcggcgcc gcaagtctcg aggtctatcc aactggtagt catggcctgt gaaggcgagg
    13441 gcggatttcc cgcgaccacg ctgattaatc cgacgattgt ttattatggt ccgcaacagg
    13501 ttgaaaactg ggagggctgt ctgagcctag acggcttgcg aggaaaagtg accaggcccg
    13561 ccctcgtgcg agtgaaggcg ttggacagga atggcaagcc actcgacctt gaagccgacg
    13621 gcctctattc cgtctgtatc cagcacgaga tggaccattt aattgggaaa gtctttgttg
    13681 accggatgac ggatctgtcc acgctaaccc agttgaatga gttcgacaag tactggcgga
    13741 aagagcctac ccccgttatc tgagccaccc ttcgattgat cgctgtgaaa acgctattgc
    13801 gagtcctcca gtatcttcgt ccccatcgct cgatggtcat cggcacgttt ctttttgcag
    13861 gactgacgac ggcgttcgaa ctggtcccgc cttggctgat taaggtgctc atcgacgatg
    13921 ttattcaggc cggcaagaca cagctcctgt cgctggtctt cggcggactg tgtaccgcct
    13981 acgtgctgcg tagcgtgtgc gcctcaatga gaatcaagct gaacaacagc ctggagcagc
    14041 agatcgttca cgacttgcac gtgcaggtct tcggcgcgtt gcagcgtctg tcgatcagct
    14101 tttttgagaa ccgccccacg ggtgaaatca tgtcgcgcgt gctcaacgat acggagcaca
    14161 tgcaacggat tttcgtcgac ggcctggaag agatcatcac cgcgggcctg accttgatcg
    14221 gtatcatggc cgtcctcttt tggctcaact ggaagttggc gttgctcgcg ttggtgccga
    14281 ttcctcttct cgtgctcgga gcgatcgcct ttacgaaacg aatccacaag tattaccgga
    14341 aaatacggaa aggatccgcg gagttgaacg ctctgctaca agatacactc gcggggattc
    14401 gtgaaacgat ggggttcaac cggcagccct acgaggagga ccgtttcggc cgtaagagcg
    14461 atcaatgccg caaggacaca ctcaaagcga tgtacctctg gtcctattac tcgccgggga
    14521 tgatgcttgc cggcagcctt ggagcggcgc ttgtcctgtg gaacggcacg catgaggtcc
    14581 tgggacagcg cctctccgtg ggcgaattgg taatgttcat gtcctacctg gccttattct
    14641 atgtgcccat caatcagatt cattcggtca atcacatgtt gcagcatgcc cttgcagcaa
    14701 gcgaacgggt ctttgaagtg ctcgacatcg tgcctgaggt gaaggatgag ccgggactcc
    14761 gggctccggt gcctcgcttg accggtgaaa tcgcctttcg cagggtacac tttcattatc
    14821 gcccagatgc acctgtgcta tacgacgtgt cgctcatcgt gcgggccggg gagagagtgg
    14881 cgcttgttgg cccgagcggc gccgggaaga gtacgacgtt aaaactgctg acccgtttct
    14941 acgacgtcac gggaggggag atcaccatcg acgggcacga ccttcgagaa atgccgctcg
    15001 cctttctccg cagccagatc ggccttgtcc aacaagaacc cttcctattc aacggcacgg
    15061 tgcgggagaa tattttgtac ggcaatctct cggcgagtca tgacgacgtg gaggcggctg
    15121 ctacagcggc gcgtgcccat gaattcatcg tcaacctgcc cgaaggatac gatacctgga
    15181 tcggcgagcg cggggtgaag ttgtcagtcg gtcagcgaca gcgtgtgtcg attgcccggg
    15241 tgctattaaa agatcctccg attgtcatgt tcgacgaagc gacgtccaac atcgacaccg
    15301 agacggaagt aaagattcga gaagcgctgg aggagctcac caaagacctg acgaagattg
    15361 tcatcgccaa taggctttcg accgttcacg ggatgtaccg gatcattgtg ctggagcatg
    15421 gccgtgtggt cgaggagggt ccccatgagg tactcatcac ccgaggcggt gtctatgccg
    15481 gactttatga ggcccagttc cagacttagc aacgtcccag atctcctcac ggactacgcc
    15541 tgacggcatt cctatggtct tgatctacga aaacgatccg ctggataatg aagatgctcg
    15601 cggacgcttt tatcatgtct gtcgagggac gatcgtgcgg acggagatac aaataccatc
    15661 gggtgcgacg gtgtacgaat gcgctgtctg cgggattgac ctggagccgg aagatttctg
    15721 gctcgcccga cagaaaggat cggtgtagat atgactcgca gtgcaggcag gaaaaccgtc
    15781 gcggtcatga ggggactgat gatgctgtgt gacagttgct atgatcaggt cctcgcagaa
    15841 cacctgaccg accacaagaa tttcgtgcaa aactacgagg ccctgttcga caccatttgt
    15901 cccggctgta ccgacatcaa tcgtccgttg gtcgacgaca tgctgggcag ctcggagtag
    15961 tccctggcac ggtgcaacgc ctatctattt acactccttc ccgttgaagt acatgcaggt
    16021 cgattctcct gatttcacct tccacgtttt cacgagcggc atctgaccat cgcctcgcat
    16081 ttcaacgacg aagtccacta aacctgaaat cggcagcttc tccatgtgcg gctgagcggc
    16141 cgccgggacg tcgacgtaga acacggaacc gagcgtggaa atcagaatgc ggtggttgtc
    16201 tttatcgact tggatgacgg gactgtaata acttttgtct tctgtcaggc ccggacgggc
    16261 aatcagcagc actgcgacgg cgagaagaat cgtacgcatg taggccatga cgatcacccc
    16321 ttctcaaaaa ggtcgaagat ggcgtcatta tacgcaccgg tccaaaaaga aggaaactgc
    16381 tatttcgtaa ccagcggatg agaggatcga cttacagggt gacgacgtcg gcggaagaga
    16441 ccgaggcgtt cttgatccag taggcccgca cgtccgggcg ggctttgtcc atcaaggaaa
    16501 cgagcatgta gatcggcaca ctcaaattaa gccggctcca gttctcttcc cactcgttcg
    16561 caattttgat gtcggtgacc gacggcctgg ccggatcgtg agggtgcgaa tgatagacga
    16621 cttgcagtgt caaaccgtgt tttctcatgt ctttgaccgc ctgaaacatc tcttgggcat
    16681 ccatgatgaa cgcaatctcc gcccgctctt ccggtgatag ctgtgagaga tggttcatct
    16741 tggcttcatc gaaggaagcc gcctcggaag cgccgtccaa actcaccaca ttcttgatcc
    16801 gatagagccg tgtcaccgtt ttgtctgtgc cggccaaaaa tccacagcac tcgtatggat
    16861 cgagttggcg cgcgtgagcg acgaggtcgt tcataatctg tcggggaatg gtcaggtcgg
    16921 acacgttata gcgagcaact gacggtgtaa tcggtgctga gatctttgat gcgaggattt
    16981 gggccgcaga gaggacagtc gggatctttt gggcgactga cctttctgaa cttcatctcc
    17041 agggcgtcgt aaatcaacag cttgtcagca agcgtctcgc cgatggcgag tatttcctta
    17101 atcgcttcgg acgcctgtaa gattc
//
LOCUS       DASSCQ010000182         7675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_777869, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000182 DASSCQ010000000
VERSION     DASSCQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777869"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFH05_12005"
     CDS             <1..744
                     /locus_tag="VFH05_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6678989.1"
                     /translation="QGVKDFKYYEPWTSQEFAQASVDRGQGLYKEYCAQCHGFTGKGD
                     GPAASGLEPKPAIHANIPFEKLPIEYLYNVVNHGGAAMGKSPSMPYWNLTIGQQGVAD
                     VIAYLKATFKGAPDMATAPAGGQGDACIQPRKTAKAPEEFFTKTNPLPASEGTIQAGK
                     TLFLKTAQPVACAMCHGDQGNGKGFMGAALTPPPRNFTCGAMMKNIPDGQLFWVIKNG
                     SPGTGMMSFAGLPDDQVWQLIHYIRSLAK"
     gene            822..1220
                     /locus_tag="VFH05_12010"
     CDS             822..1220
                     /locus_tag="VFH05_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6678990.1"
                     /translation="MGGYVVNIEEATATNVNYRRVLFTAKHAQLVLMNLMPGEEIGEE
                     VHELDQFIRFEAGEGSVILDGKTHSVSDGFAVVIPAGTRHNVVNRSKTADLKLYSLYS
                     PPEHKDGTVHKTKQEADADEHHHFDGKTSV"
     gene            complement(2343..2825)
                     /locus_tag="VFH05_12015"
     CDS             complement(2343..2825)
                     /locus_tag="VFH05_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6678991.1"
                     /translation="MNTLLRWDPTSRTRWNPFKDHDHNELESRLATLLATREGMGNGE
                     KEALTVTQWSPLVDISEDDKEYLIKAELPDMKKEDVRLTVEDDVLSISGDRKFEKEEK
                     GRKYHRIERAYGSFLRSFSLSEDADGRNVTANFKDGVLQVHLPKSVKAKQKAVEVKIG
                     "
     gene            complement(2881..3111)
                     /locus_tag="VFH05_12020"
     CDS             complement(2881..3111)
                     /locus_tag="VFH05_12020"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HET6678992.1"
                     /translation="MMREKVSNPTTPGVSASRSTGSQGDEQSDPPFQISKAGWDYERI
                     ARRAYELYEQRGRQEGRDLEDWMKAEQQLAGE"
     gene            complement(3221..3388)
                     /locus_tag="VFH05_12025"
     CDS             complement(3221..3388)
                     /locus_tag="VFH05_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678993.1"
                     /translation="MLYYALVFLAVGLITGALTITGMSSLPVEIPLVLSLTGIVLVAT
                     YMFVRRTARIA"
     gene            complement(3944..4090)
                     /locus_tag="VFH05_12030"
     CDS             complement(3944..4090)
                     /locus_tag="VFH05_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678994.1"
                     /translation="MVSPYRSPIVPLLGQLTTVLYREVMYAAATSAPDMHTKKSASKS
                     SGAM"
     gene            4089..4991
                     /locus_tag="VFH05_12035"
     CDS             4089..4991
                     /locus_tag="VFH05_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6678995.1"
                     /translation="MSKERITISPDLYDIMPSDYQDLVNHATYGKEDRGWKDIGSSKE
                     LIEQHSLCAGCPESMAFRYVIAALPNPEDTVMVGSTGCTSLVFPHVAIHNIHSLFGNQ
                     NAVASGLKRALTVRFPNRIKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYIVNCTVSKPSVVEKV
                     IRNAVLIAREIGPTYVQLYTPCILEIGKDSMEGLQEMRDSEKPNERFAFKEYVSDPAK
                     ALLAELAAKAKERKAAAKTVLQEA"
     gene            5326..5562
                     /locus_tag="VFH05_12040"
     CDS             5326..5562
                     /locus_tag="VFH05_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678996.1"
                     /translation="MANHTESLAPVNSQYTLGYGMRMDEGTAPELAHPNVSVILPDGS
                     EGHMTLHVINGTPAEIKARLLESVDAFFDIHAEL"
     gene            5585..6253
                     /locus_tag="VFH05_12045"
     CDS             5585..6253
                     /locus_tag="VFH05_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678997.1"
                     /translation="MQTLNLHRPHMPDLQFVLLVTALCTSRLPALNIPNGLRTTIFDR
                     CWMLIHDSPPPRKPEERVLDLRPWSEMTVEAMVETIRATLTEAGIETLAWNHPPGDPT
                     PTSTPEAQMLIDRLERLYPHPSEEKDAASGTVLETTGVHPDPGDNEVWQLIGEMATLM
                     AALAPAMKRRAADLAAGGKAGEVVDKLLKGAEVMHGSGHMYLTWARHYASLSENAAEE
                     AQDT"
     gene            complement(6388..6783)
                     /locus_tag="VFH05_12050"
     CDS             complement(6388..6783)
                     /locus_tag="VFH05_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6678998.1"
                     /translation="MSIACPAGLLRILVADDFAPFRESLCRLFQDYKSLTVVGAAVDG
                     EAAVEMAFQLLPQIIVMDVKMPRISGIEATRRIKRFLPDVHIIGISTQDDTAVKESMK
                     AAGSSHFVTKDCAHTLPDIISSIAGWPIT"
     gene            6979..7278
                     /locus_tag="VFH05_12055"
     CDS             6979..7278
                     /locus_tag="VFH05_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6678999.1"
                     /translation="MKHTSHPNIRKELHECFHHCERLLASALMSDNPPFSQEELAMMK
                     YYAEEIGKISVSHDTFPISESRPVRGLRESHTSSLNRGWGGWEHHESGWWNVGKR"
BASE COUNT         1798 a         2117 c         2104 g         1656 t
ORIGIN      
        1 cagggtgtca aagacttcaa atactatgag ccctggacga gtcaggaatt cgcgcaggca
       61 agcgtcgatc gcggccaggg gttgtacaaa gagtattgcg cacaatgcca tggctttacg
      121 ggaaagggcg acggacctgc cgcgagtggt cttgaaccga agcctgccat tcatgccaac
      181 attccgttcg agaaattgcc gatcgagtac ctctacaacg tcgtgaacca cggcggcgcg
      241 gcgatgggca agtcgccgag catgccctat tggaacctga cgatcggcca gcagggcgtt
      301 gccgatgtga ttgcatactt gaaagcgacg ttcaaggggg cgccggacat ggcgaccgcg
      361 ccggcgggag gacagggcga tgcctgtatc cagccgcgca agactgccaa ggcgcccgaa
      421 gaatttttta caaagacgaa tccgctgccc gcctctgagg ggaccattca ggcagggaaa
      481 acactctttc tgaagacggc acagcctgtc gcttgtgcca tgtgtcatgg ggatcagggc
      541 aatggaaagg ggtttatggg tgcggcactc actcccccgc cgagaaattt cacctgtggg
      601 gcgatgatga agaacattcc agacggtcaa ttgttctggg tcatcaagaa tggatcgcca
      661 ggaaccggca tgatgtcttt cgcaggattg ccggacgatc aggtctggca gttaattcac
      721 tatatccgga gcctggcaaa gtaaccgaat catggcatcg acttctttca cagccgtaat
      781 tggtggtggc gttgaattga tgtaagaagg gaggcgtgtt tatgggcggg tacgtggtca
      841 acattgaaga ggcgacagcg acaaatgtga attatcgccg ggtacttttt acggctaaac
      901 atgcgcagct cgtcctcatg aacttgatgc cgggagagga aatcggagaa gaggttcacg
      961 aactcgacca atttatccgt tttgaggccg gcgaaggttc cgtgattctc gatggcaaga
     1021 cacattctgt ctcagacggg tttgcggtcg tcatcccggc aggcacaaga cacaatgtgg
     1081 tgaaccgttc caagacggcg gacctcaagc tatatagcct gtacagtcct ccggaacaca
     1141 aggacgggac tgttcacaaa accaagcagg aagctgatgc cgatgaacac catcacttcg
     1201 atggcaaaac gtccgtgtga gaggcctgct gacgagcatg atccaagtaa ccgatggagc
     1261 cgcctgaaag ctgtgggaat tcgtccgctg tcggtgggcc agtgatttta ggagtccgcg
     1321 attgggacct aaacctgatg gcctcgcctt aaaagttcaa ttgaacccgt tttaggggac
     1381 acactcgttt gttgcaaatc atttccccaa cgtttatttt cgaagcgaca gccgccctcc
     1441 agtacagcgc acctagtcct cggccctgca ataaggtggt tccacaaaaa tacgtgtaat
     1501 ttccaccggc tgtctctatt gaacagacaa agggaagagc ggctcgctat actattctcg
     1561 caaaccttac gcgagcggtc agacagaacc tactaacttt gaattgcaag aaaggaaaac
     1621 catgaagtca tccattaccg cgcatgaaaa ggccaatctt gcatttcttg gaatgttggg
     1681 aatattcatc gtgactctgc tatacggcgt gttcgcgatg ggaatacgat aaactttatt
     1741 gtactggtcc tgacagaact attctgcgac ggtctatgcc tttaccggac aggcaagact
     1801 tctggcaaac aggtcctcat ccaaggtgcc ccctcaactt ccctactcct acctgccgct
     1861 gattccatga cgggtcccct ggacgcgggt cccctggaca tggagttggt ggttcatagt
     1921 ttgctttggc acctgttcgc ttccaagatg caccgccagt caatgaacct ttcgttctca
     1981 accggagcca tttgcagcgt taagccacct ccgtggtttt gagactggag ggatgaggat
     2041 gattcgacga tggagagtct acgtccttat tggctaccca acacatacca aagcagcatg
     2101 aaataagctc ccaagatgcc catcataagg gcaaagagtg ttgccgcaag agcccaattt
     2161 tcactcacga tagtcatggc gtgcattcct ttttcacaag aagcgagaat gatggtcctt
     2221 accacggtcc gcttcacggg tttggagagg aactgacgac ggtcgcgcgc gtggagcgtc
     2281 agcaaaactc catcgtcgca tgcgcgtacg aagcctcacc gacgccccac gaatcagcac
     2341 cactagccaa tcttaacctc cacagctttc tgtttcgcct tgacggattt ggggagatgc
     2401 acctggagca cgccgtcctt gaaattggcg gtcacgttgc gcccatccgc atcctccgaa
     2461 agggagaagc ttcgcaggaa gctcccgtag gcgcgttcga tgcggtgata ttttctgccc
     2521 ttttcttcct tctcgaattt ccgatcaccg gaaatcgaca gcacgtcgtc ttcaaccgta
     2581 agccgcacat cctctttctt catgtccggc aactcggctt tgatgaggta ttccttgtca
     2641 tcctcggaaa tgtccaccag cggcgaccac tgggtgaccg tgagggcctc tttttcgccg
     2701 ttccccatcc cctcccgtgt cgcaagcaac gtcgccaacc ggctttccaa ttcattgtga
     2761 tcgtgatctt tgaaagggtt ccatcgagtt ctgctggtgg ggtcccagcg taatagcgtg
     2821 ttcatggctc gtctcctttg atgtcgaatg gagttcccat tcgaccaccg cttcatgtga
     2881 ttactcgccg gccagttgct gctctgcttt catccagtcc tctaggtccc ggccttcctg
     2941 ccgccctcgt tgctcataca gctcgtaggc ccgccgggca atgcgctcgt aatcccatcc
     3001 ggccttcgat atctgaaaag gcggatcgga ctgttcatcc ccctgactgc ctgtggatct
     3061 gctggccgac acaccgggcg ttgttgggtt cgaaactttt tccctcatca tcgtgcacct
     3121 cctcacgcct ccgtgtggtt cgtgggggaa tgacttcccc acttcctttt caggttgtct
     3181 ttgaactcta tccgttgtcc cttgagtgct cagcaccccg tcacgcaatc cgagcggtgc
     3241 gtctgacgaa catatatgtc gccaccaaca cgatccccgt caacgacaac accaagggaa
     3301 tttcgaccgg taacgacgac attccggtga tagtcagggc accggtgatc agaccgaccg
     3361 ccagaaagac tagtgcatag tagagcatgg ttcactcctc tctatggaag cgagcgtgaa
     3421 tcgaccagcc tctgacgcag tggccggggc cttcgccgcc tccatcagcg gatcaggaaa
     3481 ccggacaggc aaatgagtca gggcacccgg tgtagcaaaa cggcggagac tggggaatcg
     3541 gtctcgctgg gaaggagtaa gaagcgggag gataggacag tcaacggagc gttacacctc
     3601 atcaacaacc ctgggcgcaa tgggccaggg agtcaagcga tataataagg acgcgatcat
     3661 agaatggatg aactaaacac caagctaccc tttatagcgc ttgacacctg tgtctatagc
     3721 gcttagcgtg aagctatgac gtattaagca cgccgactgt cctgtccctc gcgcgttcct
     3781 tccttcctgc gcgactgatt tcctggtctc cgccgcttct acgccgaatg tcttttcaca
     3841 acgtgccgtg aattcgcatg acccgaggcg atgccatccc tctggcgaag aagtgcctcg
     3901 gcacgcaagt cgatcgaagg gttgcgcaga tggactttat tcgtcacatg gcaccggaac
     3961 tcttgctggc gcttttcttg gtgtgcatgt caggagcgct ggtggcggcg gcatacatca
     4021 cttcgcggta gagcacagtc gtcaattgcc caagcaacgg cacaatcgga cttctataag
     4081 gagacaccat gagcaaggaa cgcattacca tatcaccgga tctttacgac atcatgccat
     4141 cggattatca ggacctcgtc aaccatgcca cgtatggcaa ggaagaccga ggatggaaag
     4201 atatcggcag ctcgaaggaa ctcattgaac agcactcact ctgtgccgga tgtccggagt
     4261 ccatggcgtt tcgttatgta atcgccgcgc ttcccaaccc ggaagatacc gtcatggtcg
     4321 gctcgacggg gtgcaccagc ctggtctttc cgcacgtcgc catccacaac atccattcat
     4381 tgttcggcaa tcagaacgcg gtggcctccg gcctgaagcg ggccttgacc gtccgttttc
     4441 cgaatcgcat caaggatgtg gtcgtgctgg cgggagacgg cgcaacggtg gatatcggct
     4501 tggacatgac gctccaagcc tggttccgcc aggaaaagtt tacgaccatt tgcttcgaca
     4561 acgagttgta cgctaatacc ggtgggcagg aaagcggcct gatgcagaag ggcttcgtag
     4621 ccaagatggc cccggtcgga aaactcttcg ataaagtccg cctccccgag atcgcgcgcg
     4681 aatccgggtg ccactacatc gtgaattgca cggtgagcaa gccctcggtc gtcgagaaag
     4741 tcatccgcaa tgcagtcttg atcgcacggg aaatcggccc cacctacgtc cagctttata
     4801 cgccatgcat cctcgagatc ggcaaggaca gcatggaagg cctgcaggaa atgcgtgatt
     4861 ccgaaaagcc gaacgagcgc tttgcgttta aggaatacgt cagcgatcct gccaaggcgc
     4921 tgctagcgga actcgccgcc aaggcgaagg aaaggaaggc ggcggcgaag acggtattgc
     4981 aagaggcgta atttaccgag gcgattgcga ctcacagcga aagagcgagg tgttcggatg
     5041 agtctcgata atgtgaagat ttcactgaga cctgacgcgt tcatgctcaa gaaacctcgc
     5101 gggctcttcg caaagacggc gcggtatgga tgcagggagt gtaaccagaa gccggcggcg
     5161 gcgattggaa cggcatgagt acgagaaggc taccctccag cctacttggt cacttccacg
     5221 ggttggattg gagtagggtc ggaatgacct acagcgcaaa cacggaatgg aatgaacgcg
     5281 acgtcacccg tcttcaacac actgccattt gcagaaggag catccatggc caaccacaca
     5341 gaatctctcg cgcccgtcaa ttcgcaatac acgctgggct atggcatgcg catggacgag
     5401 ggcacagctc ccgagctggc gcatccgaac gtgtccgtga tactgccgga cggatcggaa
     5461 ggacacatga ccttacacgt cattaacggc acaccggccg aaatcaaagc gcgattgctc
     5521 gaaagcgttg acgcgttctt cgacatccat gccgagcttt gagggaagac gggacacatc
     5581 caccatgcag acgttgaatc tccatcgtcc ccacatgcct gatctgcagt tcgtgctgtt
     5641 ggtgacggca ctctgcacct ctcgcctgcc cgcgcttaac atcccgaacg ggcttcgcac
     5701 aacgattttc gatcgctgtt ggatgctcat ccacgacagc ccgccgccgc gcaaacccga
     5761 ggagcgggtg ttagacttgc ggccctggtc ggagatgacc gtggaggcaa tggtggagac
     5821 catccgcgcg acactcaccg aagcaggcat cgaaacgctg gcctggaatc acccgcccgg
     5881 cgacccgaca cccaccagca caccggaagc gcagatgctc atcgatcgcc tcgagcgcct
     5941 gtatccgcac ccctccgaag agaaagacgc agcaagtgga acggtgttgg agaccacagg
     6001 agtccacccc gatcccgggg acaatgaagt gtggcaactc atcggtgaga tggccacatt
     6061 gatggccgca ttagcccccg caatgaaacg ccgcgccgca gacctcgcgg cgggaggaaa
     6121 agctggcgag gtggtcgaca agttgctgaa gggcgccgag gtgatgcacg gcagcggaca
     6181 tatgtatctg acttgggcac gccactatgc ctctctcagt gagaatgcag ccgaggaggc
     6241 ccaagacacc tgagccatga acgccttcga gaaatagccg tccaaactta gggagaggca
     6301 atcgtacgcg tggttgcctt cgctcatccg gttatctctt aacgtgctgg gttcttttct
     6361 ggtcaagagg acctttaaaa aaggtggtca cgtaatgggc caaccggcaa tgctggaaat
     6421 gatatcagga agggtgtggg cacagtcctt cgtgacaaag tgcgaggatc cggcagcttt
     6481 catggattct tttacggcag tgtcatcctg cgtggagatt ccaatgatgt gtacgtctgg
     6541 aagaaaacgt ttgatccgcc gagtggcctc gattccactg atccggggca ttttcacatc
     6601 catgacaatg atctgaggaa ggagttgaaa ggccatctca acagctgcct ctccgtccac
     6661 ggccgcaccg acaacggtta agcttttgta atcttgaaag agacgacaga gggactcccg
     6721 aaatggggca aagtcatcgg ccacgagaat gcgaagcagt cctgctgggc atgctatcga
     6781 catcgctgta atattttctg cctttgaatc actcttgtct gttcaaaagg agacacagac
     6841 ggtctgagtg ttgagtgcta actcgcaggc ttgacacagc ttttgagtga gcccagacta
     6901 ccttgacaca accacagcct gccgacggcc tcttggaagg cccgaccgcc gacaccttgt
     6961 gagaaaggcg gtcgatctat gaagcacact tcacatccaa acattcgcaa agaactccac
     7021 gagtgtttcc atcactgcga gcggctgtta gccagcgctc tcatgtccga caatccacca
     7081 ttttcccaag aagaactggc gatgatgaag tactacgcgg aggaaatcgg taagatttcc
     7141 gtttcccacg atacatttcc aatttcggag agcaggccgg ttcggggtct ccgagagagt
     7201 cacacgtcgt ctttaaatcg tggatgggga ggctgggaac atcacgagtc gggatggtgg
     7261 aatgtcggaa agcgctaaga attacggcct ccgtgaagag cccgagcgcg gtcttttgta
     7321 ggcaggaaag gcggtcgccc atgcagagtt cttcggcgtc tacatcatcg gcttgcaggg
     7381 ttctcttaat cgaggatgac ccagaacaat tggccttttg gtcagctgca ctcagaaatt
     7441 gcgcatccca ttattcggtt ttagaggtgg caggcggtca ggagggcctc gaactgcttc
     7501 ggcaccagaa agtagattgc gtagtcttgg atttggatct gtctacctca tcaggtttcc
     7561 aagtcttgtt ggacatagtc cccgaccgaa aacggccaaa gaccgccgtg ctcgttttca
     7621 ctcgtctaca gaatcccact ctggcaaaga tgtcactgga gaatggtgcc caagc
//
LOCUS       DASSCQ010000183        10788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_644525, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000183 DASSCQ010000000
VERSION     DASSCQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644525"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(580..993)
                     /locus_tag="VFH05_12060"
     CDS             complement(580..993)
                     /locus_tag="VFH05_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679000.1"
                     /translation="MADPKSILLIDGSARDRRYYGSVVQGALPDCRILEAENGEDGLK
                     LYQSQSIDCVILELALPDIHGTDLLKQLDVPGCLARKPVIVLTRLFYPTILTIARENG
                     AIATFVKKVTSEQVLQDAVLAALSIIPASLSAEKG"
     gene            1645..1932
                     /locus_tag="VFH05_12065"
     CDS             1645..1932
                     /locus_tag="VFH05_12065"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET6679001.1"
                     /translation="MRGSRKLLIGLAAMTMGACAEEHGKSYWLRHATLTLSQAAQIAE
                     KNGPGRAVRAELGQSGTRVFYDIEIIDTFNKSRRLRVDAETGKIIQGLGLP"
     gene            complement(1979..2055)
                     /locus_tag="VFH05_12070"
     tRNA            complement(1979..2055)
                     /locus_tag="VFH05_12070"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2019..2021),aa:His,seq:gtg)
     gene            complement(2132..2842)
                     /gene="pyrF"
                     /locus_tag="VFH05_12075"
     CDS             complement(2132..2842)
                     /gene="pyrF"
                     /locus_tag="VFH05_12075"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET6679002.1"
                     /translation="MKVVARDRLILALDLPSAVDAERLMDQVQDQVTFVKVGLELYTA
                     AGPDIVQRLLKRGKRVFLDLKFLDIEETVRRATERVAAMGVEFLTVHANRKALAAAVK
                     GRGDSALKLLAVTVLTNFDSHDLREMGIQHTVQELVTARALLAAEMGCDGVVASGEEP
                     GVLRPKVGPRFVIVTPGVRPAGEDVDDHARSTTPTQTIAAGADYLVIGRPIRAAADPA
                     AAAAAVLSEMQAAFDARA"
     gene            2938..3213
                     /gene="rpsT"
                     /locus_tag="VFH05_12080"
     CDS             2938..3213
                     /gene="rpsT"
                     /locus_tag="VFH05_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET6679003.1"
                     /translation="MPVIHKSTIRRARQSEKRQARNRAALSAVKSLVKKVHAAVTDKK
                     ADEAKASLQIATSALSKAVSKGVLKPNTASRRISRLTRHVNSLVASR"
     gene            complement(3188..4279)
                     /gene="holA"
                     /locus_tag="VFH05_12085"
     CDS             complement(3188..4279)
                     /gene="holA"
                     /locus_tag="VFH05_12085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET6679004.1"
                     /translation="MPQTVPVSQLPAHLTKVVAPLYAVLGEEDLLRDTALALIRDAVL
                     GADGNDFNCDVFYGDEAEGAEIAACASEIAAFAPRRLVIVKAADKLAARQSEMLLPYL
                     KSPNDTTSVVFVAAKLDGRLKFSQALLQASVVVDCGPLKDSQLLPWLQQEADRLGVRI
                     DEEALHLLREVSGGSLSAVRRELEKLASYVPADRAATAADVAALRGTQPGASVFDLAA
                     AIGAQQRGATLAILARNLEAGEAPLRILGSLVWQYRRLWKVKELVRQAGREGEAARVL
                     RMDPYRIRPFLGQFSETHLREAFRLFLETDQKLKGGSGGKPAMLLDQLMLKLCSRPQP
                     TRSPSPAAPASRGRTLSNVRTVSVTRPKN"
     gene            complement(4291..4926)
                     /gene="lptE"
                     /locus_tag="VFH05_12090"
     CDS             complement(4291..4926)
                     /gene="lptE"
                     /locus_tag="VFH05_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HET6679005.1"
                     /translation="MHGRAANQMHSLVALSARLSILSLCLPLLVSCGYQFRVEGPGPT
                     IGGTASAAPSTPPPRVVIRPLRNATFEPNIEARFTNYLRHEFSSGSGAQVVPEGEAAD
                     LVLSGDILAVILPTLSFTQTTTLESRAEVIVAVRVEETRTKRLVWSQVAKGASEFYVT
                     PDLQFNRVLQNRAVEQAGRYIAEDLASRFLLQLESGQLSKPVVDTKPEQKP"
     gene            complement(4958..7393)
                     /gene="leuS"
                     /locus_tag="VFH05_12095"
     CDS             complement(4958..7393)
                     /gene="leuS"
                     /locus_tag="VFH05_12095"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET6679006.1"
                     /translation="MSKTYDHRAIEAKWQAYWEAHRPFRAVEDPAKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMKGFNVLHPMGWDAFGLPAEQYAVKTGIHPAITT
                     AQNIATFKRQMKRVGLSYDWDRELSTTDPGYYRWTQWIFLQLFKRGLAYVAEVPVNWC
                     PALGTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYADRLLDDLSLVQWPPSTLE
                     MQKNWIGRSIGAEVEFGIQGINGTIRIFTTRPDTLFGATYIVLAPEHPLVEVVTPATE
                     REAVNAYRQAAARKSDLQRQELDKEKTGVFTGGYAINPVNQERLPIWIADYVLMGYGT
                     GAIMAVPAHDARDWAFAKTYHLPIREVIAGGDVQKEAFVETDKGVVVNSTSPDGRFSI
                     DGLAPVDAIPKITAWLESVGTGRKSVNYKLRDWLFARQRYWGEPFPIVWVDGEARPLP
                     EEQLPLALPETKDFTPSGNGESPLANLEHWLKTTDPESGEPARRETNTMPQWAGSCWY
                     YLRFLDPKNNEQVVDPGKEQYWMPVDLYIGGSEHAVLHLLYSRFWHKVLFDIGVVSTP
                     EPFRKLVHQGIVLGEDNQKMSKSRGNVVNPDEMIDRFGADAVRLYEMFMGPLEAMKPW
                     STRGVEGVTRFLERVWRLMVDEAGQLSSAIVDSVPPVEQQRILHQTIKKVTEDIEHLR
                     FNTAISQMMVFTNEMTKLPQRPRTLIEPFLLLLSPFAPHLTEELWSIIGNSPSISQQP
                     WPSFDSALTMSDRLTIPVQVNGKLRGKLEVSTDADRQQVQDLAREVAAEWLTGKELKK
                     VIYVEKKLINFVV"
     gene            7546..8121
                     /locus_tag="VFH05_12100"
     CDS             7546..8121
                     /locus_tag="VFH05_12100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6679007.1"
                     /translation="MSGQVWQGLRDDLLKDAHGDVLEIGFGTGLNLPHYPATVSQLWM
                     VDPAGFLPSRVRARVAQAAFPVHMEQLTAESLPFPERRFDSIVSTWTLCSIPDPLKAL
                     REIRRVLKPQGVFLFLEHGRSREARIAAWQDRLNPIQNLIACGCHLNRPIDLLIERSG
                     FHLTDLSRFQVPHVPRIIGEMFQGHARTTRC"
     gene            complement(8128..8787)
                     /locus_tag="VFH05_12105"
     CDS             complement(8128..8787)
                     /locus_tag="VFH05_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6679008.1"
                     /translation="MLNWDGIDDVLLDMDGTLLDRHFDNFFFEEELPRRYATAQGLSF
                     EASRACLMAMYRSVEGQLAWTDLHYWSDRVGIDVVAMHKELDHMIGFLPGAEAFLQEL
                     RTRGKRIIVVTNAHQAGVAVKSAKTGLSRYVDRIVDAFEVGYLKMRSEYWPACRRLLK
                     FDPVRSLYIDDDESCLHAARHYGIGSIIHSAKSSSQLPAVYSTNFASVDRLSTLLTRT
                     L"
     gene            8879..10276
                     /gene="der"
                     /locus_tag="VFH05_12110"
     CDS             8879..10276
                     /gene="der"
                     /locus_tag="VFH05_12110"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET6679009.1"
                     /translation="MLCYGPITVSTTDPTPTPSVLSPQPTIVAPTVAIIGRPNVGKST
                     LFNRILGTKTAIVDDVPGVTRDRNYADANYRNRPFRLVDTGGLEPSASEGMLALIKRQ
                     SELAIEEAEILILLMDGRTGLMPQDREVVHLLRGTTKPLFVAVNKIDTPKVEPLVADF
                     YQLGIEQLFPVSAEHGIGISELLDAIYPLLPAPEEQTGPHGMPRIAVVGRPNVGKSTL
                     VNAVLGEERVVVSDVPGTTRDAIDSLAVYDGRRYLFTDTAGIRRRGKVDRGIEGHSVA
                     RSLRAIGRSDVAVLLLDAEEGVTEQDTKIAGVVIRQGRACVLLANKWDRREADPLARQ
                     KFEKDLKRRFPFLSWAPVLFGAAVQPDSLRRLFPLIDQVFHSFSKRIPTGQLNQFLQN
                     LLAVHPLPVRKGKPAAITKSAFMTQVAVRPPVFALFVGHPDNITPTYLRFLENQIRET
                     YGFAGTPLRLLVRKK"
     gene            10394..>10788
                     /locus_tag="VFH05_12115"
     CDS             10394..>10788
                     /locus_tag="VFH05_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679010.1"
                     /translation="MKTSRRIIRGFRNPGRLLPLRLLLFISLAAGSSPAGLWAAEVGQ
                     ANDASPQTTAPDSQADAAPVEVDPSITLKGAVWRTKAGIVFLKTPVGLLTLSSKTTLK
                     DLKASHEVSFWVHDRHSAVEIRKRSDGSLV"
BASE COUNT         2353 a         3181 c         3009 g         2245 t
ORIGIN      
        1 ctgggggaga cacttattca ttcaaatcaa gcatcccaac gaaggagccg caagcctatg
       61 agaacgcgcc tttccttttc cgttgtagcg atcaccctca gcctcgtggt tgcatgcgtg
      121 gaccatcggc cgggatctgt cgatgtcgtc aaggatcaat ctaaggatca atctggaggg
      181 tcaaacatga gcccaagtca aatcgctgaa gcacaaaaat tggcgcgtga gtggaatgtc
      241 agaatgagcg cccgacagag ctaagagaga ctgtgatggt ggctgtgaga ggaaattgtg
      301 tctctttcgg gcgatcaaaa gcggaagcga agataggaag tcttatggca tcggcagcac
      361 tgccagggat accaccccag gctgctcagg atgtggtccc tccattgatg acgagccaaa
      421 ccctttagca cgcattgccc cttatgcttt gacgtcagcg gaatgaagtg tctctgccgg
      481 gaaaaggttc ggttcgtcga ggaggaaatc tgacgggtgt agtacatgat ccatgtcaga
      541 gtagaactgg gaaatgtttg cgaggaatcc actctctcat caacctttct cagcgcttaa
      601 tgaagcagga ataatcgaga gggccgcaag cacagcatcc tgtaggacct gttccgaagt
      661 cactttcttg acgaacgttg cgatggcgcc gttttccctg gcgattgtta atatagtcgg
      721 ataaaagagt cgagtcagca cgataactgg ttttcgggca aggcagcccg gaacgtctag
      781 ctgtttaagc aaatcggtcc catgaatgtc gggcaacgcc agttcgagaa tcacgcaatc
      841 aatcgactgc gactggtaaa gtttcaggcc gtcctctccg ttctccgctt ccaagatacg
      901 gcaatcgggg agcgcacctt ggacaacact gccataatac cgtcgatcgc gggcactgcc
      961 gtcgatcagg agaatggatt taggatccgc catgcacaac cgcccttgtc aggatgtcgg
     1021 cggtcgggcc ttccaggagg ccggcggcat ctgagatgag aagtgaaggt atattctgaa
     1081 ctcctttatg agctggcgtc aattgaaaac atgcaacaaa aggagctgcg gccgcagcca
     1141 gctcatcacg ccatcaggac accggcaaat accgcactcc acaaggagtc agacacgtac
     1201 gtggagagaa gctgtgcgag gacctcgcct cccctcttca catcatcggg tatccttctg
     1261 agtgggcggc ctcactaaac acagatctct caccaggatg ggtttaaact cgggcagctt
     1321 tgtctctagg agatcagcgc gtcattgctt tgaatgttca gaaatattgg ctttagttga
     1381 gccacctggt atgacaaacc aagcatgttt gacggaccca cagacacgag gttatggaca
     1441 gaccgcaatg tgatctcgag gtgttttcct ccgttcatgg gcagacgtat ggcgagtatt
     1501 acgccatgtt gcctcatggg cgaaccgcgg tgtcccctca taccactgca agccccacga
     1561 ctaccggcag gcttgccaag cctgatcaag gagcgattca cgttggcgat catctcgtct
     1621 catacttgtc ataacggata aaacatgcgg ggatccagga agctgctgat cggattggct
     1681 gccatgacga tgggagcttg tgcggaggag cacgggaaaa gttactggct gcgtcacgcc
     1741 acgctgacgc tctcgcaagc cgcacagatt gcggagaaga atggacctgg gcgagctgtg
     1801 cgagccgagc ttgggcagtc tggaacccgc gtcttttatg atatcgagat catcgatacc
     1861 ttcaataagt cccggcgcct acgtgtggat gctgaaaccg gaaaaatcat ccaaggtttg
     1921 ggtctgccct agttccctac caggcgtgct ggagtacttt gtaattcgga agaaaggctg
     1981 gggtggatga cgggtttcga acccgcgacc tccggatcca caatccggcg ctctaaccaa
     2041 ctgagctaca cccaccgtgc agggactgag gggaaaactg aggaaattag atcttagcaa
     2101 agtgagaaga acggccgcaa ctcatcggcc gtcaggctct ggcatcaaac gccgcctgca
     2161 tttcactcag aaccgccgca gctgcggcag cgggatcggc agcggcgcga atgggacgac
     2221 cgatcacgag ataatcggca ccagccgcga tggtttgagt tggagtcgtc gacctcgcat
     2281 gatcgtcgac gtcttcaccg gcggggcgga cgcccggagt cacgatgaca aaccgtggcc
     2341 cgactttcgg cctcagcacc cccggctcct cgcctgatgc cactacaccg tcacaaccca
     2401 tctccgccgc caataacgcg cgggccgtca ccagttcctg cacggtgtgt tgaataccca
     2461 tctcgcgcaa gtcatgactg tcgaaattgg tgaggaccgt cacagccagc aacttgaggg
     2521 ccgaatcgcc ccgacctttg actgcagccg caagcgcttt gcgattcgca tggacggtca
     2581 gaaactcgac gcccatcgct gccactcgtt ccgtggcgcg ccgaaccgtt tcctcaatat
     2641 ccagaaactt caaatcgaga aagacgcgct tgccccgctt gaggagacgc tgcacgatgt
     2701 ccgggccagc cgctgtatag agctcgagcc ctaccttaac gaaggtgact tgatcctgca
     2761 cctgatccat caaccgttcg gcatccaccg ccgaaggtaa atccaaggcg agaatcaacc
     2821 ggtcacgtgc gacgaccttc accgcagtat cctcccacca cttttctcga ctcgtctaag
     2881 gttgacgatc cgctgaagcc tatggtacaa atgcgctcct ttgtacagga gtttgctatg
     2941 ccagtcatcc ataaatcgac gatccggcgg gcccgtcagt cagaaaagcg gcaggctcgc
     3001 aaccgcgccg ctttaagtgc cgtgaagagc ctggtgaaaa aggtgcacgc ggcggtcact
     3061 gataagaagg ccgacgaagc caaggcctct ttgcaaatcg cgacatcggc tctcagtaaa
     3121 gccgtcagca agggcgtcct caaacccaac accgcatccc gccgcatttc gagactgacc
     3181 agacacgtca attctttggt cgcgtcacgc taaccgttcg aacattcgac aaggtcctcc
     3241 ctcgcgacgc cggagcagca ggcgatggcg agcgtgtcgg ctgtgggcgg ctacacagct
     3301 tgagcatcag ctgatcgagc aacatcgccg gcttccctcc gctaccaccc ttcaatttct
     3361 gatcagtctc caaaaacaag cggaacgcct cccgcaggtg tgtttccgaa aattgcccaa
     3421 ggaagggtcg gattctatag gggtccatcc taagcactct ggccgcctcg ccttcacgac
     3481 cagcctgtct gaccagttcc ttgaccttcc agagccgccg atactgccag acgagagaac
     3541 ccaagatccg caagggcgct tcccctgcct ctagattgcg agccagaatg gccagcgttg
     3601 ccccgcgttg ttgcgcgccg atcgcggcgg ccaaatcaaa aaccgaagcg cccggttgag
     3661 tgccgcgcaa tgccgcgaca tcggctgccg tggcagcacg atcggctggc acatatgatg
     3721 ccaacttctc caattcccgc ctcaccgcag agagcgaccc gcccgagact tccctgagca
     3781 aatgcagcgc ctcttcgtcg atccggacgc ctaagcgatc cgcctcttgc tgtaaccagg
     3841 gcagcaattg cgaatccttg agcggaccac aatccacaac gactgacgcc tggagtaacg
     3901 cctggctgaa tttcagccta ccgtcgagct ttgcggcaac gaagactaca ctcgtggtgt
     3961 cattgggtga cttgagatat ggcaacagca tttcagattg tctggcggca agtttgtccg
     4021 ccgccttcac aatcacgagt cgacgcggtg caaaagcggc gatctccgac gcacaggctg
     4081 cgatctccgc tccctctgcc tcatcaccat agaatacatc gcaattgaag tcgttcccgt
     4141 ccgccccaag caccgcgtcc ctgatcaggg ccaaggcggt gtctctcagc aggtcttcct
     4201 cgccgagtac ggcatagagc ggtgccacga ccttggtgag gtgcgcaggc aactgagaaa
     4261 cggggactgt ttgaggcata ggggatccct ttagggtttt tgctccggct tcgtatccac
     4321 gaccggcttt gacagttggc cggattccag ctgcaacaaa aatcgagatg caagatcctc
     4381 ggcaatatat cgtcccgcct gctcgacggc ccggttttga agcacccggt tgaactgcag
     4441 atccggcgtc acgtaaaact ccgatgcacc ttttgccact tgcgaccaga cgagacgctt
     4501 ggtcctcgtc tcctcgactc tcacagcgac gatcacttct gcgcgacttt ctagcgtcgt
     4561 ggtttgcgtg aaactgagag tcggcaagat gaccgcgagg atatcgccgg ataggaccag
     4621 gtccgccgct tcgccctcgg gcacgacctg cgcaccgctg cccgacgaaa actcgtgccg
     4681 gagataattg gtgaaccgcg cctcaatatt gggttcgaag gtcgcattcc ttaatgggcg
     4741 gatcactacg cgaggcggag gcgtcgacgg cgctgcggag gccgttcctc ctatcgtggg
     4801 gccgggtccc tcaacgcgaa attgatagcc gcagctgacc aacagtggca ggcagagaga
     4861 cagaattgag agtcgagcac tcaatgcgac aagcgagtgc atctgattgg ccgcgcgtcc
     4921 gtgcattcgt ttgcgatccc tcagctcacc gcggtcatta gacgacaaag ttgatgagct
     4981 ttttctcgac atagatgacc tttttcagct ctttccccgt cagccattct gcggccactt
     5041 ctcgggccaa atcctgaacc tgctggcgat cggcatcggt actcacctcg agttttcccc
     5101 gcagcttccc gttcacctga accgggatgg tcaaccgatc actcatcgtc agggcagaat
     5161 caaaggaagg ccatggttgc tgagagatgc tcgggctgtt tcctatgatc gaccatagtt
     5221 cttccgtcag atgaggagca aatggggaca ggagcaggag aaatggctca atcaaggtcc
     5281 gtggtcgctg aggaagcttc gtcatctcat tggtgaagac catcatctga gagatggccg
     5341 tgttgaatcg gagatgttcg atgtcttcgg tgaccttctt aatcgtttga tggaggatcc
     5401 gctgttgctc gactggaggg acggagtcga cgatcgcaga cgagagttgg cctgcctcgt
     5461 cgaccatcaa acgccagacc cgttcgagaa agcgggtgac gccctctacg cctcgcgtac
     5521 tccagggctt catggcttcc aatggtccca tgaacatttc atagagtcga acggcatcgg
     5581 cgccgaagcg atcgatcatc tcatcggggt tcacgacgtt gccgcgagac ttggacatct
     5641 tctgattgtc ctcacccaac acgatgcctt ggtgaacgag cttcctgaac ggctcgggtg
     5701 tcgagacgac gccgatatcg aacaggacct tatgccaaaa tcgggaatac agaagatgca
     5761 gtaccgcatg ctcgctgcca ccaatgtaga ggtcgaccgg catccaatac tgttctttcc
     5821 cagggtcgac gacctgctcg ttgtttttcg gatcgagaaa acgcaaatag taccagcagg
     5881 acccggccca ttgcggcatg gtattcgttt cgcgccgcgc cggctccccg ctctccgggt
     5941 ctgtcgtttt gagccaatgt tccaggttgg ccagcgggct ctccccgttg cctgacgggg
     6001 tgaaatcctt cgtctcaggt agcgcgagtg gcaattgttc ttccgggagg ggtctggctt
     6061 cgccatccac ccatacgatg ggaaacggct ctccccaata gcgctggcgg gcgaacagcc
     6121 agtcgcgaag cttgtaattg acggacttgc gccctgtccc aacggattca agccatgcgg
     6181 tgatcttggg aatggcatca accggcgcga gtccgtcaat cgagaagcgt ccgtcgggcg
     6241 atgtcgaatt gacgaccacc cctttgtcgg tctcgacgaa ggcctccttc tgcacatcgc
     6301 cgcctgcaat gacttctcgg atgggcaagt gataggtctt cgcgaatgcc cagtcgcgcg
     6361 catcgtgcgc cgggacggcc ataatggcac cggtgccgta gcccatcagg acgtaatcag
     6421 cgatccagat gggaagccgc tcctgattga ccggattgat ggcatagcct ccggtaaaca
     6481 ctcccgtctt ctctttgtcc agctcttgcc gctgtaaatc gcttttgcgg gcggccgcct
     6541 ggcgataggc attgacagcc tcacgctcgg tggcgggcgt cacgacctcg acaagcggat
     6601 gctccggggc gaggacgatg tacgtcgcgc caaacaacgt atcaggcctg gtcgtaaata
     6661 ttctgatcgt tccgttgata ccttgaatgc cgaattccac ttccgcacca atggaacgcc
     6721 cgatccaatt cttttgcatc tccagggtgc tgggcggcca ctgcaccagc gacaaatcgt
     6781 caagcaaccg gtccgcatag gccgtgattt tgagcaccca ctgccgcatg ggcttgcgga
     6841 tgacgtcgaa gccgccgact tcacttttcc cgtcgacgat ttcttcgttg gccaacaccg
     6901 ttcctagcgc cggacaccag ttgacgggaa cttccgcgac gtaggccagg cctcgcttga
     6961 acaattgcag gaagatccat tgcgtccacc ggtaatagcc ggggtccgtc gtactcagtt
     7021 cgcggtccca gtcgtacgac aatccgacac gcttcatttg ccgtttgaag gtggcaatat
     7081 tctgcgccgt cgtgatggcc ggatggattc ccgtcttcac cgcatactgc tcagcaggca
     7141 atccgaaggc gtcccagccc atgggatgca gcacgttgaa gcccttcatc cgtttatacc
     7201 gggagacgat atcggtcgcc gtatagcctt cgagatggcc gacatgaagg cccgacccgg
     7261 agggataggg aaacatgtca aggcagtaaa atttgggctt ggccggatct tcaaccgccc
     7321 tgaacgggcg gtgcgcttcc cagtaggcct gccacttcgc ttcgatggcg cgatgatcgt
     7381 aggtcttact catgcggtcg ggatcggtct ggaagaggaa ggactctaac acagacttct
     7441 cgaacgcaac aacattcgga gggcctcatc agggccgctt tgctatactg catcccgatg
     7501 gggttgtacg gccagcacgt gtttccccgc cttatggact gggtcatgag cggccaggtg
     7561 tggcaaggcc tgcgtgacga tctgctaaag gacgctcacg gggatgtctt ggaaatcggc
     7621 ttcggcaccg gactgaacct gccccactac ccggcaacgg tttcgcaact ttggatggtg
     7681 gaccctgccg gcttcctgcc gagccgggtg cgagcgcgag tggcgcaagc ggcctttcct
     7741 gttcacatgg agcagctcac tgcggaatca ctcccgtttc ccgaacgacg attcgattct
     7801 atcgtcagta catggacgct ttgctccatt cccgatccat tgaaagccct tcgcgagatc
     7861 agaagggtgc tcaaaccaca aggcgtattt ctctttcttg agcatggacg cagccgggag
     7921 gcgcgcatcg ccgcctggca ggatcgactc aatccaatcc aaaacctcat tgcatgcggc
     7981 tgtcatctga accgcccgat cgatttactc atcgagcgaa gtggatttca cctcacggac
     8041 ctcagccgct ttcaggttcc gcatgtgcca cgcatcatcg gggagatgtt tcaaggtcac
     8101 gcgcgcacga cccgctgctg accacgctta caacgtgcgg gtcagtagcg tcgagagacg
     8161 atccacggat gcgaaattgg tggaatagac ggcgggtaat tgcgaactgg atttagcgct
     8221 gtggatgatc gacccgatgc catagtgcct ggctgcatgg aggcagctct cgtcatcatc
     8281 aatatagaga gaccgcactg gatcaaattt tagcagccgt cggcaggccg gccagtattc
     8341 cgatcgcatc ttgagataac ccacttcgaa ggcgtccacg atccgatcga catatcggct
     8401 gagaccggtt ttggccgatt tcactgccac accggcctga tgcgcattcg tcaccacaat
     8461 aatccgtttg cctcttgtcc ggagctcctg taaaaaggcc tcggctcccg gcaaaaaacc
     8521 gatcatgtgg tccagttcct tgtgcatcgc cacaacatcg atgccgaccc ggtcgctcca
     8581 gtaatgcaaa tcggtccagg caagctggcc ttcaacggac cgatacatcg ccatcaagca
     8641 cgcgcgagaa gcttcgaatg agaggccctg ggccgtcgca tagcggcggg gcagctcttc
     8701 ctcgaagaaa aaattgtcga aatggcgatc gagcaacgtg ccgtccatgt ccagaaggac
     8761 gtcgtcgatc ccgtcccaat tcagcatggg gatgggccgg atgttgattg ttgctgaatc
     8821 atgagcagag tatatcgaag cgtcaaaagg aagacgagac gggttgtctt tgagaaaaat
     8881 gctgtgctac ggtccgatca ccgtgtccac tactgatcca acacccacgc cttcagtgct
     8941 cagccctcag ccgacgatcg tggctcccac agtcgccatc atcggacgac cgaacgtggg
     9001 caaatccacg ttgttcaacc ggattcttgg gacaaaaaca gcgatcgtcg acgatgtgcc
     9061 gggagtgaca cgggaccgga actatgccga tgcgaactac cgaaaccggc cctttcgtct
     9121 ggtcgatacg ggtgggcttg aaccgtcggc atcggaaggc atgctggcgc tgatcaaacg
     9181 acagtccgaa ctggccattg aggaagcgga gattctgatt ctcctcatgg acggtcggac
     9241 cggactgatg cctcaggacc gtgaggtcgt ccatctgttg cgcgggacga caaagccgct
     9301 gttcgtcgcg gtcaacaaaa tcgatactcc gaaggtggag ccgttagttg cggattttta
     9361 tcaactcgga atcgagcagc tgttccccgt ctcagcagag catggcatcg gaatctccga
     9421 actgctcgat gccatttacc cgctgctgcc ggcgccggaa gagcagacag gaccacatgg
     9481 catgcctcgc attgccgtcg tcggacggcc gaacgtcggc aaatccaccc tggtcaatgc
     9541 cgtcctcggc gaggagcgcg tcgtcgtcag cgacgtaccg ggaacgacga gggatgccat
     9601 cgactcgctg gccgtctatg atggccgtcg atatttgttc accgacacgg ccggcatcag
     9661 acgccggggg aaggttgatc gcggaatcga aggccacagc gtcgcgcgtt cgctcagggc
     9721 catcggacgg tctgacgtcg ccgtcctctt gctcgatgcc gaagaaggcg tgacggaaca
     9781 ggacaccaag atcgccggcg tggtgatacg gcaggggcgc gcctgcgtgt tgttggccaa
     9841 caaatgggac cggcgcgagg ccgacccgct ggccaggcag aaatttgaaa aggacttaaa
     9901 acggcgattt ccgtttctct catgggcgcc cgtgctcttc ggggccgcgg tacaaccgga
     9961 ttctctccgc cggctttttc cgctcatcga tcaggtcttt cactcatttt cgaagcgcat
    10021 ccccaccggc cagctcaatc agttcctgca aaatctctta gcggtgcatc cactaccggt
    10081 gcggaaagga aaaccggcgg ccattacgaa atcagccttc atgactcaag tggcggtcag
    10141 acctcctgtg tttgcgctct tcgtcggcca tccggacaat atcacgccga cctatctccg
    10201 cttccttgaa aaccaaatcc gcgaaacgta cggattcgca ggcacgcccc tccgcctgct
    10261 cgtcagaaaa aaatagccag agccattcca tcagtggcca tgctcgggcc ggccaacgac
    10321 agacagcgct ccgagggctt cagcttgact ctgcgacatc atcgtggtta attcgcgtcg
    10381 ggcacggtcg tcaatgaaga cttcacggcg tatcattcgc ggtttccgca atcccgggcg
    10441 actattaccg cttcggctgc tgctgttcat cagcctggcg gccgggtcct cgcccgctgg
    10501 gctttgggct gccgaggtgg gtcaggccaa tgacgcgtct ccccagacga ctgcacccga
    10561 ctcccaagcg gacgctgcgc ctgtggaggt ggacccttcc atcacgttga agggcgcggt
    10621 gtggcgaacc aaggcaggga tcgtcttctt gaaaactccg gttggactgc tgaccctcag
    10681 ctccaagacg acgttgaagg acttgaaagc ctcccacgag gtctcgttct gggtgcatga
    10741 ccgccactcc gccgtcgaaa ttcgcaaacg aagcgacgga tcgctcgt
//
LOCUS       DASSCQ010000184         5161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_454218, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000184 DASSCQ010000000
VERSION     DASSCQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454218"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFH05_12120"
     CDS             <1..391
                     /locus_tag="VFH05_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET6679011.1"
                     /translation="QLRMDVIRMIKAAVLNKEVELKKDLDDAEMSRIMTTMIKQRKES
                     VEQFEKGQRAELAAKERQEISIIETYLPQALSAEELAQAVDAVIRETGATSAKEMGLV
                     MKAVMARLAGQSVDGKSVSDLVRSKLK"
     gene            473..1111
                     /locus_tag="VFH05_12125"
     CDS             473..1111
                     /locus_tag="VFH05_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679012.1"
                     /translation="MRILIVDDSPDQQTLLRSILSKAGHTDVLSADGATSAAKVLNLD
                     GDSIPEHIDLILMDVLMPGQDGVETCRQIKRCAHLQDIPVIMVTAKSDLSNLQDAFAA
                     GAMDFISKPVSSIELLARVSSALLLKQEMDRRKNRELELRRSNEELQKALKEVKVLRG
                     LIPICASCKKVRNDGGFWQQLEEYLGEHSEAEFSHGLCQPCIKKLYPGVYQD"
     gene            1168..2172
                     /locus_tag="VFH05_12130"
     CDS             1168..2172
                     /locus_tag="VFH05_12130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET6679013.1"
                     /translation="MSILIVDDSPDDRTLLQSILTAAGYEHILVAESAVTAFHLLGLD
                     GGKQGAARVDLILMDLIMPEMNGIEACRQIKAVERFRETPIMMVTVKTDPVDLQLAFA
                     AGALDYIGKPINKIELLTRVRSVLRLVHEIERRRAREQELLEVMRQLQEANQMLLRLS
                     SLDGLTGITNRRQFDDFLDQEWRRAVRESTPLSLIMLDIDRFKIYNDTNGHTAGDECL
                     RQVANAISGAVNRPGDLVARYGGDEFVVVLPGTATDGAAQVAETLRKRIETLGITHSE
                     GDHITISVGYASIIPNRNASPTDLIKAADQALYQAKQEGRNRAKQAGILTLVPHSDDE
                     "
     gene            2202..3941
                     /locus_tag="VFH05_12135"
     CDS             2202..3941
                     /locus_tag="VFH05_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679014.1"
                     /translation="MPRPHASSRKPPRTSRRPTLAQTQAALRRERATRRREALQHRQT
                     EQALVVAKEAAEVANKAKSDFLASISHEIRTPMNAIIGMADLLWETDLTAEQRKYLRI
                     FRRAGGSLLQLINDILDLSKVEAGHIELESIEFDLGDLVEKAIEILAMRANEKGLELA
                     CHLAPDVPCSLIGDPNRLHQILVNLISNAIKFTETGSVIVEVTNDADEAAPGTIRFSV
                     TDTGIGIPQDKLSTIFERFTQAHASISRQFGGTGLGLTISKQLVGLMHGSIWAESRPG
                     QGSTFHCRVKLGINPSATIRPAAFPLDLHNLRTLVVDDHAMNRKILMETLKSWNADVT
                     TVGDGQQALAEVRQAALSSRPYGLLLVDCHMPGMDGFQVVEALRQLALAEGVAIIMLA
                     SDHWADDIARTYDMALGGYLTKPIRRSDLLQTVSIALDRAHGVSATPTARATASAPIA
                     SRPLHILLVEDSPDNQVLVRSYLNATPYQLDVAEHGGIAIEAFKKRPYDVILMDMNMP
                     VMDGYEATRNIRAWERTHDLPKTQIIALTALVMKEDGEKILQAGCNAHMTKPIKRQTL
                     LEVLHACEGYRPK"
     gene            3938..4261
                     /locus_tag="VFH05_12140"
     CDS             3938..4261
                     /locus_tag="VFH05_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET6679015.1"
                     /translation="MTTNGNSPERVMVAVDPDLQDLVPGFLENRRKDAAALDRAAQAK
                     DFSTVRLLGHRMKGDGGGYGFQEISRIGNALEQAAIREDWNTIRQETAGLIAFLAQVD
                     VVYRR"
     gene            4431..4730
                     /locus_tag="VFH05_12145"
     CDS             4431..4730
                     /locus_tag="VFH05_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679016.1"
                     /translation="MAQQERTQEWRGRDARGVVSQNLDSVESGVDEAIDSAKEAVHEF
                     RGKAEEVADAVLDRVNRSWERQRPRIEAYMTSHPWIVFGGLILLAYLFSAKERTR"
     gene            complement(4842..>5161)
                     /locus_tag="VFH05_12150"
     CDS             complement(4842..>5161)
                     /locus_tag="VFH05_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679017.1"
                     /translation="CGDGLVEDRFMDWTARWRCFKCGHVQDSLSVKSLPTRPEESRAM
                     THAEPDYRDEEVHLGSESFVGNVTYGYHRATVQINRHSQKLQPPRSKNTRRLGQQRNP
                     ISS"
BASE COUNT         1170 a         1516 c         1443 g         1032 t
ORIGIN      
        1 tcagttgcgg atggatgtca tccgcatgat caaggccgct gtgctgaaca aggaagtcga
       61 gttgaaaaag gacctcgacg atgcggaaat gagccgcatc atgaccacca tgatcaagca
      121 gcggaaagaa tcggtcgagc aattcgaaaa gggccagcgc gccgagctcg ccgcgaagga
      181 acgacaagaa atttccatca tcgaaaccta cctgccccaa gccctctccg ctgaagaact
      241 cgcccaagcc gtcgatgccg tgatccgcga aacgggtgcc acgtccgcca aggagatggg
      301 actcgtgatg aaagccgtca tggctcgtct cgccggccaa tcggttgatg ggaagtccgt
      361 cagcgacctc gttcgctcca aactcaagta agccaatcgt cgcagagtcg tctgctatac
      421 ttggtggcgt actaatggct ccccgcatta ggaaccacca cgtaggagtt tcatgcgtat
      481 tctgatcgtt gacgactcac cggatcagca gaccctcctg cgctccatcc tgagcaaagc
      541 cggccataca gacgtcctta gcgctgatgg ggcaacgagc gcggcaaagg tgctcaattt
      601 agacggtgat tccatccccg agcacatcga tcttatcctg atggacgtcc tgatgcccgg
      661 acaggacggc gtcgagacct gccggcaaat caaacgctgc gcacacctcc aagacattcc
      721 cgtcatcatg gtgacggcca agagtgatct ctcgaatctg caagacgcgt ttgccgccgg
      781 ggcaatggac tttatcagca aaccagtcag cagcattgag ctcctcgcgc gcgtgtcctc
      841 cgctttgctg cttaagcagg aaatggatcg ccggaaaaat cgagagcttg aactgcgccg
      901 cagcaacgag gaactgcaaa aagcgctgaa ggaggtgaag gtgctacggg gtcttattcc
      961 tatctgcgcc tcatgcaaaa aggtcagaaa tgacggcggg ttctggcagc agttggaaga
     1021 gtatctcggg gagcattccg aggccgaatt cagccacggc ctctgtcaac cctgcattaa
     1081 aaagctttat cctggagttt accaggatta gctgttacga tttctttacg taccgcgacg
     1141 tccctctcaa gccgaggcgc catttccatg agcatattga tcgttgacga ctctcccgac
     1201 gaccggaccc tgcttcagtc gatccttacc gccgcaggct acgaacacat cctggtcgct
     1261 gaatcggctg tgaccgcctt tcatctcctg ggcctcgacg ggggcaagca aggtgccgcc
     1321 cgcgttgatc tcatcctgat ggacctcatc atgccggaaa tgaacggtat cgaagcctgc
     1381 cggcaaatca aagccgtgga gcggttccgc gaaaccccga tcatgatggt caccgtgaag
     1441 accgatccgg tggatttgca gttggccttc gccgcagggg ccctggatta tatcggcaag
     1501 cccatcaata aaattgagct cttgacgcga gtacgatcgg tgctgcggct cgtgcacgag
     1561 atcgagcggc ggcgggcacg cgagcaggag ttgctggagg tcatgcgcca gctccaagaa
     1621 gccaatcaga tgcttcttcg gctctcttct ctggacggct tgaccggcat cacgaaccgc
     1681 cggcaattcg acgatttcct cgatcaagaa tggcggcgtg ccgtacgtga atcgacgccg
     1741 ctatccctga tcatgctcga tatcgaccgg ttcaagatct acaacgacac caacggccac
     1801 acggccggcg acgaatgtct acgccaagtt gccaatgcca tctctggggc tgtcaaccgg
     1861 ccgggcgatt tggtcgcccg gtacggcggt gacgagtttg tcgtggtgct tcccggcaca
     1921 gcgacagatg gagctgcgca agtcgcggag accttgagga agcggatcga aacgcttggc
     1981 atcacccact cggaaggcga tcacatcacg atcagcgtgg gatatgccag tatcattccc
     2041 aatcgaaatg cctcccctac cgatttgatc aaggccgcgg accaagcgct ctatcaagcc
     2101 aaacaggaag gccgcaaccg ggccaagcag gccggcattc tgacactcgt tccccactca
     2161 gacgacgagt agtccggccg cgccaccacg cgcccccgac catgcctcga cctcacgcgt
     2221 catctcgaaa gccccctcga acctcccgcc gtcccacact tgctcagaca caggcggcgc
     2281 ttcgccggga acgggccaca cgccgacggg aagcgcttca acatcgccaa accgagcagg
     2341 cgttggtcgt ggcgaaggaa gctgcagaag tcgcgaacaa agccaagagt gactttctgg
     2401 cgagcatcag ccatgaaatc cgtacaccga tgaacgcgat catcggcatg gccgatctgc
     2461 tgtgggagac ggacctgaca gccgagcagc gcaagtacct tcgcattttc cggcgcgccg
     2521 gcggcagtct cctgcagttg atcaacgaca ttctggacct ctcgaaggtc gaggccggtc
     2581 acatagaatt ggagtcgatc gagttcgatt taggggatct cgtcgaaaaa gccattgaga
     2641 ttttggcgat gcgcgccaat gaaaagggct tggagctcgc ctgccatttg gcgcctgacg
     2701 ttccctgttc gttgatcggc gaccccaacc ggctccacca aattctcgtc aatctcatca
     2761 gcaatgcgat caaattcacc gagaccggat ccgtcatcgt cgaagtgacg aatgacgccg
     2821 atgaggcagc gccggggacc attcgttttt cggtcaccga cacgggaatc ggcatccctc
     2881 aagataaact gtcgacaatc ttcgaacgct ttactcaggc ccatgcgtcc atcagcagac
     2941 aattcggcgg caccgggctc ggcctgacta tcagtaaaca gctcgtgggg ttgatgcacg
     3001 gctccatttg ggcggagagt cgccccggac aaggcagtac ctttcactgc cgggtcaagc
     3061 ttggaatcaa tccgagcgca acaatacggc ctgcggcctt tccactcgac ttgcacaact
     3121 tgcgcacgtt ggtggtggac gatcacgcga tgaatcgcaa gatcctgatg gaaacactga
     3181 agtcatggaa tgccgacgtt acgaccgtgg gcgatggaca acaggccttg gcggaagtac
     3241 ggcaggcagc tctgtcttct cgcccctatg gtcttcttct cgtggattgt cacatgccgg
     3301 gcatggatgg attccaggtc gttgaagcgc tccgtcaatt ggctctggcg gagggagtgg
     3361 cgatcatcat gctggcttcg gatcattggg cggatgatat cgcccggacg tatgacatgg
     3421 cgcttggcgg ttatctgacg aaacccattc gccggtccga cctgctgcag accgtcagca
     3481 ttgccttgga tcgagcacac ggagtctccg cgacgccgac ggcgcgcgcc acggcctcgg
     3541 ctccgattgc ttccagaccg ctgcacattc ttcttgtgga agactctccg gacaaccagg
     3601 ttcttgttcg ctcctatttg aatgccactc cgtatcaact cgacgtggcc gagcatggtg
     3661 gcattgccat cgaggcattc aagaaacgac catatgacgt gatcctgatg gatatgaata
     3721 tgccggtcat ggatggctac gaggcaacca ggaacattcg agcgtgggag cggacgcatg
     3781 accttcccaa gacccaaatt atcgcgctga ccgctctggt catgaaagaa gacggagaaa
     3841 aaatattgca agccggctgt aatgcgcaca tgacgaaacc catcaagcgg caaacgctcc
     3901 tggaagtcct tcacgcctgt gaaggctacc gacccaaatg accacaaacg gcaatagtcc
     3961 agagcgtgtg atggtcgcgg tcgatcccga cctgcaagac ctggtgcccg gattcctcga
     4021 aaaccgtcgc aaggatgcag cagcactgga ccgcgcggcg caagccaagg atttcagcac
     4081 cgtgcgtcta ctcggtcacc gtatgaaggg ggatggcgga ggctacgggt ttcaggagat
     4141 cagcagaatc ggcaacgccc tggagcaagc cgcaatcaga gaagactgga acaccattcg
     4201 ccaggaaacc gccggtctca tcgcctttct tgctcaggtc gatgtcgtct atcggcgctg
     4261 aagcggccga cagctttcaa tacgtcgtcg gacggcaacc cttccaagtc acgtgccacc
     4321 tcactccgca acttcgagat ggtgacccac tccctccatc tagggaccag gagagctgtt
     4381 cctggcgccg tttcttgaaa tactgaacct ctcaccgaaa ggaggacctg atggctcaac
     4441 aggaacgaac gcaagaatgg cgcggaagag atgcccgagg tgtggtcagc caaaatttgg
     4501 acagcgtcga atcgggggta gatgaggcaa tcgatagcgc caaagaggcg gtacacgagt
     4561 ttcgcggtaa ggcagaagaa gtcgccgacg ccgtattgga ccgagtgaac aggtcctggg
     4621 agcgccaacg gcctcgaatc gaagcctata tgacgtctca cccctggatc gtgttcggag
     4681 gactgattct gttggcgtat ctgttttccg cgaaggagcg gacacgttag agtgccgctc
     4741 ttcgaataga gctgtcgcct cgtagtcgtg tctccttctc tcactcgggt agtacgatct
     4801 acgaggcggt gtgttcaaga taagaacaca tcctgtatag gctacgaact gatgggattc
     4861 cgctgttgcc ctagccgccg ggtgttctta cttcgtggtg gttgtaactt ctgactgtgt
     4921 cgattgatct gcactgtggc tcggtgatag ccgtatgtga cgttccccac gaatgattcc
     4981 gatccgagat gcacttcctc gtcccgatag tccggctcgg cgtgtgtcat ggccctgctc
     5041 tcttccggcc gcgtcggaag gctcttcacg ctcagcgaat cctgcacgtg gccacatttg
     5101 aaacaacgcc atctggccgt ccagtccata aagcgatcct caacaagccc atctccgcaa
     5161 c
//
LOCUS       DASSCQ010000185         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_989270, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000185 DASSCQ010000000
VERSION     DASSCQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989270"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1372
                     /gene="tyrS"
                     /locus_tag="VFH05_12155"
     CDS             161..1372
                     /gene="tyrS"
                     /locus_tag="VFH05_12155"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET6679018.1"
                     /translation="MKELSHQLNLILRGVVEVIQQAELESKVTRSIKEHRPLRIKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHHVIFLIGDFTGTIGDPTGVSETRKVLTKAQVE
                     DNAKTYQRQIFKILDPQKTTIEFNSRWMGPMKADGLIELAAHYRVARMMERDDFQKRY
                     QEQKPISVHEFLYPLVQGYDSVALKADVELGGTDQKFNLLVGRDLQRDYGQEPQVVIT
                     MPLLEGTDGVKKMSKSLGNYVALEDVPNEMFGKIMSISDDLMIRYYELLTTENLSDIK
                     RAHPMEAKQRLAKLIVERYHGEEAARQAGESFQQRFQDRGFPSEPDMRLTLTRADLNT
                     DGAIALTDLIAKTGLVPSKSEARRLVIQGGLEVDGEKQTDANTVLRPIAGRAYQMKIG
                     RRKFALIQLQD"
     gene            1485..1865
                     /locus_tag="VFH05_12160"
     CDS             1485..1865
                     /locus_tag="VFH05_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6679019.1"
                     /translation="MAHAGQIRVLIADDHTMVRQALRNALQAFPNIDVVGEAGDGDEA
                     VAATAKLQPEVVVMDVNMAKMDGITATRLIKAQHQEIAVIGLSVDLKDYQFHALKNAG
                     ASVVLSKENAALYDLYGAIQQAAA"
     gene            2860..>4269
                     /locus_tag="VFH05_12165"
     CDS             2860..>4269
                     /locus_tag="VFH05_12165"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HET6679020.1"
                     /translation="MPSPLALAQTITSSGLNTAVSGPIALPNGQTQIDITNGTRPGGG
                     GNVFHSFGNFSVPNNTTANFLNNSGLPTSNILARVTGGNISNILGTIQTTGFGNANLF
                     LMNPAGFLFGPNATLNVGGMVAFTSADYLRLADGGRFTVNANAGSADILTAAPVAAFG
                     FLGANPGAISLQGSQLVVAEGTGISLVGGDITGQGATLTAPSGQINLVGVKQISNPNV
                     PGEVLIHGSGQGATFTPTGFATFGTISLSQGSTIDVTGLLGSGSEQAPGKVLIRGGQF
                     IMNASSVLANAYSDAFGPPPGSIEVTVDHALLEHSVIRASAGTAHHGGTIMLNVDTLS
                     ATDSFILTNGVSRGDGSGGTVEIKAGTRIDLAATEVNAGTLTAQGGTVTMASPVISVN
                     GSRVNVSGGGLDGGPGGTINLAATQSVNLINTRLDAGSGNESRLVSAGAILINGGELF
                     IAQQSVISAQATLGGRILVQ"
BASE COUNT         1016 a         1149 c         1185 g          919 t
ORIGIN      
        1 tggccctacg gattatcgag tatcggaagg cacgaggtgc ctttcgagat gtcgagcaac
       61 tgcgtcgtgt aaagggaatc gggaagaaga cagtcgaccg catccgtccc ttggtccatg
      121 tgacctcgtc actgagcatg caacccggga gaaaggcggc atgaaggagc tgtcccatca
      181 actgaatctg atactccgcg gagtggtcga ggtgattcag caggccgagc tggaatccaa
      241 ggtgacgcgc tcgatcaagg aacaccgacc attgcggatc aaagccgggt ttgatccaac
      301 ggctcccgac ttgcatctcg gccataccgt cctgattcac aagctcaaac atttccagga
      361 tcttgggcat cacgtcattt ttttgatcgg cgactttacg ggcacgatcg gcgatccgac
      421 aggggtttcc gaaactcgaa aggtccttac caaggcacag gtggaggaca acgccaagac
      481 ctaccaacgg caaattttca agattttgga tccacaaaag accaccattg aatttaacag
      541 ccgatggatg ggaccgatga aggccgacgg tctcatcgaa cttgccgccc attatcgcgt
      601 cgcgcggatg atggaacgcg atgattttca aaagcgatat caggagcaaa agccaatcag
      661 tgtccacgag ttcctctatc cgctggtaca gggctatgac tccgtggcac taaaagccga
      721 cgtggaactt ggcgggactg atcaaaagtt caacctgttg gtgggacgtg atctccagag
      781 agattatgga caggaaccac aagtcgtcat caccatgccc ttgcttgagg ggaccgatgg
      841 cgtcaaaaaa atgagcaaga gcctcgggaa ttacgtggcg cttgaggatg ttccgaatga
      901 aatgtttggc aaaatcatgt ccatcagcga cgatctcatg atccggtact acgaattgct
      961 gaccactgaa aacctgtccg acatcaaacg ggcacatcct atggaagcga agcagcgatt
     1021 ggccaagctc atcgttgagc gctatcacgg tgaggaggcg gcacgtcagg cgggcgagag
     1081 ttttcaacag aggttccagg accgaggctt tccgagcgaa ccggatatgc gactcacact
     1141 gaccagggcc gatctcaaca cggacggcgc gatcgctctt accgatctga ttgccaagac
     1201 ggggctcgtg cctagcaaga gcgaggcacg tcggctggtc atacagggag gattggaagt
     1261 ggacggggag aagcagactg atgcgaatac agtgttgaga ccgatcgccg gacgcgccta
     1321 ccagatgaag atcggccgaa gaaaatttgc cctgattcaa ttgcaggact aggcacgtca
     1381 acggaaacgc gcagtttatc tagggaagct cctagttggc gaaacccgca gataagcgtc
     1441 tcctggtctc tttatccggt cttttcaatt ttatagagga cattatggct catgccggtc
     1501 aaattcgagt gctcatagct gacgaccaca ccatggttcg ccaagcgtta cgaaatgctt
     1561 tgcaagcgtt tcccaatatc gacgtggttg gagaagcagg tgacggggat gaagcggttg
     1621 ccgctacggc aaagcttcag ccggaggttg tcgtgatgga tgtcaacatg gcgaagatgg
     1681 atggtatcac ggcgactcgg ttaatcaagg cgcagcatca agagattgca gtgatcggac
     1741 tctccgtcga tttgaaggac taccagtttc acgcgctgaa gaatgccgga gcctcagtgg
     1801 tcctcagcaa agagaacgcc gccttgtacg acttgtacgg tgccatccaa caggctgcag
     1861 cgtgaactgg cgaggacgcc gctgaagctc attgtgtccc agcggccttt cgttcttaac
     1921 gacccgagag ccaagaggga ttgtgtgccg catcatcgcc catgtccgca aacatagtga
     1981 cgaggcctac gagtatttcg cgtacacatt cgaaaggtct tgagccgttc gaggagcgct
     2041 tcactcgctt agacaatttc tgaatgttgg cactcaagcc atgaggtgac gcctcacagt
     2101 gaggcgcaaa gtgtagctcc ttgtaggact gcatctccgt ctgctttcct tgtcatccat
     2161 ccccctcatt ctacctatca aaatacttcg agtgtctgag caaactccgg cttcagcgag
     2221 tcggacgaat tgtccgggcg cagcgaatcc aattgcgaga aattatggcg aatgctgtcg
     2281 cgagagacag ttcttccttc acttgcggac aagctatccc gcttactgac ctcgtctagg
     2341 ataaatactt gcttcttcgc ggtattcgat aagccagcaa gggcatgagc cttgccttgt
     2401 gaggtggacg actagagtca ccttgaccta gagggaactc catggtttca atgagagact
     2461 gcatgttccg cccttcagtc ggctcgcaga gacgcgccgc cagcccgaaa ccccctcgtg
     2521 tctcaaacga ggtacggctc tgagtgcgtc tgcgcacgag gaagagggcg gcgggtcaat
     2581 gcacgtcgcg tgcaggcgaa gcctctgcca gagctttcga ttacacagaa cccatcctca
     2641 tcctgccaaa cccaccgttt cgatcgtctt ttctccgtgc caaagcagga agccccatag
     2701 tcggtccaac cggcatcctc atgatgaatg ctggcatcag tcagcaaggg gtgcccaaca
     2761 gcgccgggga ggtcaaacat gtgcaacaac gtaaaacccc atcgtgaacc tgccaggttg
     2821 gttcggtgcc ttgcatttgt tctccctctc ctttttctaa tgccttcccc gcttgcactg
     2881 gcgcaaacga ttacatcttc tggactgaat accgcagtgt ccggcccgat tgcactccct
     2941 aacggtcaaa cccagatcga catcacaaat gggacgcggc ccggtggcgg cgggaatgta
     3001 ttccacagtt tcggtaactt cagtgtgccc aacaacacga ccgccaactt tctcaataac
     3061 tccggccttc ccacatccaa catcctggcg cgagtgacgg gaggcaatat ctccaacatt
     3121 ctgggcacca ttcaaaccac gggcttcggc aacgcgaacc tcttcctgat gaatcctgca
     3181 ggatttctgt ttggtcccaa tgcaaccctg aacgtcggtg ggatggtcgc ctttactagc
     3241 gccgactact tacggcttgc agacggcgga cgtttcaccg tgaatgcgaa cgctggctca
     3301 gcagatatcc tcaccgctgc tcctgtcgcg gcattcggct ttctaggcgc aaatccaggg
     3361 gccatttccc ttcagggcag tcagttagtt gtcgcagaag gaactggaat ctcgttggtg
     3421 gggggcgata tcacaggcca aggagccaca ctcacggctc cgagtggtca gatcaacctg
     3481 gtcggcgtca aacagatatc gaacccgaat gtcccgggag aagtcctcat acatggatcg
     3541 ggacaggggg cgacgttcac tccgaccgga ttcgcgactt ttggaaccat cagcctctct
     3601 caaggcagca ccattgatgt gacgggattg ttgggaagcg gtagtgagca agcgcctggc
     3661 aaggtgctga tccgtggagg acaattcatc atgaatgctt cctccgttct ggcgaacgca
     3721 tacagtgatg cctttggccc tccgcccggt agcattgagg tgacggtcga tcatgcgtta
     3781 ttggaacata gcgtcatcag agcttccgca gggaccgcac atcatggcgg aaccatcatg
     3841 ctcaatgtcg atacgttgtc tgccaccgat tccttcatct taaccaatgg agtttccaga
     3901 ggggacggtt cgggtggaac ggttgagata aaagcaggga ccagaatcga cctggctgct
     3961 acggaggtga atgcgggtac actcacagct cagggtggaa cggttaccat ggcgtccccc
     4021 gttatctccg tgaatggtag tagggtcaac gtatcgggag gcggattgga cggtggtcct
     4081 ggcgggacga tcaacctggc ggcaacgcag agcgtgaatc tcatcaatac cagactcgat
     4141 gcaggcagtg gaaatgaaag ccgccttgtg tcagccggtg ccattctcat caatggggga
     4201 gagttgttta tcgctcaaca gagcgtcata tcggcacaag cgacacttgg tggccgcatc
     4261 ctggtgcag
//
LOCUS       DASSCQ010000186        11699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1295047, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000186 DASSCQ010000000
VERSION     DASSCQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295047"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFH05_12170"
     CDS             complement(<1..732)
                     /locus_tag="VFH05_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6679021.1"
                     /translation="MQPTEKVPDVRPLEPKPRVGDKDVLRNAPKEQADRGHNKSDIVL
                     LSVQRLLRRGAITNLAKMLGRMHPADVAKIIVHLSSPKEKREVFELVRGDSKRGQVLS
                     ELDTDSITQVLSDLLQSDIAWLIKDLAPDDVAYILGVLPEERAKEILSLMRTEDSTEV
                     ADILKYPKDTAGGIMTTEFFALSEDATAQDAIRRLQQATDAEMVFYIYVTDKTDHLVG
                     VLSLRQLLTVPPATPLKNIATREVIS"
     gene            complement(985..1869)
                     /gene="era"
                     /locus_tag="VFH05_12175"
     CDS             complement(985..1869)
                     /gene="era"
                     /locus_tag="VFH05_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET6679022.1"
                     /translation="MKFGTVAIIGRSNVGKSTLLNRLLNEKIAIVSEKPQTTRTRILG
                     VVHAPGAQIALLDTPGLHKPQHLLNRRMVRTAVETLEEADVLFVLMDATSLPGPGDLL
                     VLDHVTAAVNKRRRPVLLVLNKIDLVNKIKLLPVLESYARLFAWTEVVPVSAQTGDNV
                     QRLLTVAVSLLPEGEAAYDDDTVTDQTMRTLAAEMIREKLLRQTHEEIPYSLAVEIDE
                     FIEEGKLARITASVLVERESHKAIVIGKHGERLKTVGTEARLDMERVFGMKVFLQLWV
                     KVREAWREDEHALIELGY"
     gene            complement(1866..2216)
                     /locus_tag="VFH05_12180"
     CDS             complement(1866..2216)
                     /locus_tag="VFH05_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET6679023.1"
                     /translation="MIVKRNRGRAWLDWQRRMVTGAHYVAAGMGLLLLLALCFGEMGL
                     PRFFSMRDHAQELERDIQDLQRMTKMLRGDIERLERDPARIEQLARERLGYVRKGETV
                     YQLVPDDGQERSRP"
     gene            complement(2223..3512)
                     /gene="eno"
                     /locus_tag="VFH05_12185"
     CDS             complement(2223..3512)
                     /gene="eno"
                     /locus_tag="VFH05_12185"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET6679024.1"
                     /translation="MSTIREIKGRQVIDSRGNPTVEAEVTLESGAKGRAAVPSGASTG
                     EKEAIELRDGDKKRWMGKGVSKAVGNISKTIAPELLGRDALDQVGIDQAMIELDGTKT
                     KGKLGANAILGVSLAVAKAAAIETGQPLYRYLGGTNARVLPVPLMNIINGGAHADNRL
                     DLQEFMIVPIGASHFSEAVRMSTEVFHSLKALLKKKGLSTAVGDEGGFAPDLQSNEEA
                     LTLIMEAIEEAGYQPGQDMALALDCAASELYDKNRYVLEAEKTRERSSEEMIAYYGKL
                     LDRYPILSIEDGLSEVDWKGWRLLTEKLGERVQLVGDDIFVTNVEIFAKGIDEGIGNS
                     ILIKLNQIGTLTETLEAIELAKRSGYTAIISHRSGETEDTTIADVAVATNSGLIKTGS
                     LSRTDRVAKYNQLLRIEEELGAAAVYRGREAMPGRSR"
     gene            complement(3544..4995)
                     /gene="gatB"
                     /locus_tag="VFH05_12190"
     CDS             complement(3544..4995)
                     /gene="gatB"
                     /locus_tag="VFH05_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET6679025.1"
                     /translation="MIPQSASQTGYEVVIGVEVHAQLRTQSKMFCGCGTTFGLSANSQ
                     TCPVCLGLPGSLPVINKAAVEMAVRAGLALNCRIGEHNRFARKHYFYPDLPKGYQISQ
                     YESPICEDGWIDIATGEAHKRVRIRRAHLEEDAGKNIHGAAAGVSVVDFNRAGTPLLE
                     IVTEPDMRSADEVVAYLRSLRDILMYLQVCDGNMEEGSFRCEPNLSLRPVGQQEFGTK
                     VELKNINSFKLVKDAIEYEIKRQTRVLSEGGTIQQETRLWNLDRGETAVMRSKEEAHD
                     YRYFPDPDLVPLKLEREWIEGFQGGLPELPAIRARRFVEQYGLPEYDAAVLTASKDMA
                     DYFEAATQLFNQPKTVSNWVMGELTRELNNSGTTVAGSPVSPHRLVTLLEMVEKGTIS
                     LKVARDIFPELYRSEKSPEQIVQEQGLTQVSDEEALSTIIDEMLLNNPVQVAQFKEGK
                     QQVLGYLVGQAMKASGGKANPSKVNELLKRKLG"
     gene            complement(5098..5415)
                     /locus_tag="VFH05_12195"
     CDS             complement(5098..5415)
                     /locus_tag="VFH05_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET6679026.1"
                     /translation="MGHERGHERESGAATVLAFLGGAAIGAIAALLLAPQSGRTTREQ
                     LRGYARKAEDALREAANEAGQRLEEAVNEGKEYVEARKSVLREAFEAGREAMKREQTR
                     GES"
     gene            complement(5405..5812)
                     /locus_tag="VFH05_12200"
     CDS             complement(5405..5812)
                     /locus_tag="VFH05_12200"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET6679027.1"
                     /translation="MTLIELAACLAAAAFAVLVGFVVPVLINLRKTVAESERLMTRLN
                     ADLPALSSDLRSVTQNLNALTDQARNGVEHASVLLHAVGEVGESVQEVHNVMRGSSGT
                     LLTNVASLVAGVKAATRVMRNRYRQEGEPYNGA"
     gene            complement(5856..7322)
                     /gene="gatA"
                     /locus_tag="VFH05_12205"
     CDS             complement(5856..7322)
                     /gene="gatA"
                     /locus_tag="VFH05_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET6679028.1"
                     /translation="MSLHKLSLCELQKKFTAGEVTATEIVRAYFLRIGQVEPKVKAYV
                     SQTKEAALAQASALDERLKGWRKTKPMTGMPLAIKDNLCTEGVRTTCSSRMLQQFIPP
                     YDATVIAKLRDQDYILLGKANLDEFAMGSSTEHSAFGPSRNPWNLQHVPGGSSGGAAA
                     AVAADECVAALGSDTGGSIRQPAAFCGVVGLKPTYGRVSRYGLVAFASSLDQVGPITK
                     DVPDAALLLGAIAGHDPLDSTSADLPVPDYMKALKKKDLKKLNVGVPVEYFADGLDPE
                     VESSVRAAIDELKSLGGTIKEIHLPRTDAAVAVYYVIATAEASSNLARYDGVKFGLRA
                     KETKDLLDLYMKTRQEGFGPEVKRRIMLGTYALSAGYYDAYYGKAQAVRTLICEDFRA
                     AFKDVDLIVTPVTPTPAFKLGEKSEDPLQMYLSDIYTISVNLAGLPAIAVPCGFSKAG
                     LPIGVQLIGRPFEEETLLRAAHAYEQSTQWHLKKAIVR"
     gene            complement(7328..7672)
                     /gene="panD"
                     /locus_tag="VFH05_12210"
     CDS             complement(7328..7672)
                     /gene="panD"
                     /locus_tag="VFH05_12210"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET6679029.1"
                     /translation="MLRSKIHRVTVTGAHLEYEGSLTIDEDLMEAAAILPYEAIVCSN
                     LNNGERFMTYAIGGRRGSGDIVLNGPTARKAAVGDQIIIFCYEYYGDEEIKKHAPRIV
                     RVNEKNQIVESR"
     gene            complement(7717..8010)
                     /gene="gatC"
                     /locus_tag="VFH05_12215"
     CDS             complement(7717..8010)
                     /gene="gatC"
                     /locus_tag="VFH05_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET6679030.1"
                     /translation="MKITLQDVEKVAKLARLEVSPAEKEAFAKQLSQILTHVEKLREY
                     DTKGIEPTAAVLGAVNVFREDIVRPSLAVDKALTNAPEREGDAFAVPKIIEDR"
     gene            complement(8098..9927)
                     /gene="glmS"
                     /locus_tag="VFH05_12220"
     CDS             complement(8098..9927)
                     /gene="glmS"
                     /locus_tag="VFH05_12220"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6679031.1"
                     /translation="MCGIVGYVGSQEAVPILIAGLAKLEYRGYDSAGVAVLQGEKIEV
                     RRSVGKLANLQKSLAEKAINGVLGIGHTRWATHGKPSEQNAHPHRSNGCVLVHNGIIE
                     NYQPLKQQLQREGYAFQSETDTEVVAHLIDKHLQRGLNLADAVRAATKEVRGSYALAV
                     ICEKEPERLIAARSGCPLVVGRTKQASYVASDVMAMLAHTRDVIYLEEGDVASVTQSE
                     VQVIDAEGREVSRKTSRVTWDASAAEKSGYPHFMLKEIHEQPQTILDTMRGRYSYETG
                     EADLADIGLTPSEFLAVDRIWIVACGTSWHAGLVGKYLLEEMVRTPVQVDIGSEFRYR
                     DPLVGKHDLFITISQSGETADTLAAAREAKEKGARVLSIVNVVGSTLARESDGVLYTH
                     CGPEIGVASTKAFTAQLTALYLLALHLARVRNVMNVADGKAWLDRLVRLPSLVEGVLG
                     REAEIVAIAKRYYKKRNFLFLGRGINYPIALEGSLKLKEVSYIHAEGYAAGEMKHGPI
                     ALIDRDMPVVVLAPRDRLYEKTVSNLMEVKARRAPVIALVAEGERELGRTADAVFTIP
                     DTHPLMSPILFTIPLQLLAYHIAVLRGADVDQPRNLAKSVTVE"
     gene            complement(9943..11532)
                     /gene="glmU"
                     /locus_tag="VFH05_12225"
     CDS             complement(9943..11532)
                     /gene="glmU"
                     /locus_tag="VFH05_12225"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET6679032.1"
                     /translation="MSRLSRQDATRHQEPRETADRPVPDLAAIVMAAGLGKRMHSKQA
                     KVLHPVAGQAMVLYSVQLALRVAGHHVAIVVGHQADRVSQVIKQATGERSGHAPVSIV
                     EQRELLGTGHAVLQTRPVFLESGRSTPGRFLILNGDTPLLKETTLRRLLDAHQQQQAA
                     VTILTAVLDDASGYGRVVRQASTDYVSRIVEERDADVATKAIHEINVGTYVVEGDFLF
                     SALEKIQPTNAQGEFYLTDIVHMAVEEGRPVAAVLLEDPHEGLGVNTRRQLAEAEQVV
                     RAQIRDRWLDAGVRMIDPASTWIDAEVRIGKDSVLYPNVMLEGLTVIGEDSIVRSGCR
                     ITNCNIGDRVEILDHCVFCDSEIEDDATLGPFTHLRPHVVVRKGAKVGNFVEVKKTEL
                     GEGSKANHLSYLGDAKIGKGVNIGAGTITCNYDGMRKAETIVQDGVFIGSDTQLVAPV
                     TVGEGSVIAAGTTVTENVPKDSLVISRGRQVTRPGWAAKRRALFGAGVAPVSNRKQSS
                     GKSRKAASRKRTVKPSAKRSR"
BASE COUNT         2286 a         3663 c         3098 g         2652 t
ORIGIN      
        1 actgatgact tcgcgggtgg caatgttctt gagcggcgtg gcggggggca ccgtcaacag
       61 ctgtctgaga gacaagaccc cgaccagatg gtcggttttg tcggtgacat aaatgtagaa
      121 gaccatttcg gcgtccgtcg cctgctgaag gcgccgaatc gcatcctgcg ccgttgcatc
      181 ctcagacagg gcgaagaact cggtggtcat gatgccgccg gccgtgtcct tgggatactt
      241 cagaatgtcg gcaacttcgg tagaatcctc ggtccgcatt aacgagagga tttccttggc
      301 acgctcttca ggcaagacac cgagaatgta ggcgacgtcg tcgggagcga gatccttgat
      361 cagccaggca atgtctgatt gaagcaaatc ggataacacc tgtgtgatgc tgtcggtatc
      421 gagttcggac aacacctggc ctcgcttcga gtcgccgcgg actaactcga agacttcacg
      481 cttctctttt ggagaggata gatgaacgat gatctttgcg acgtcggccg gatgcatgcg
      541 gcccaacatc ttcgccagat tcgtgatggc gcctcgtcgt agcaaacgct ggacggacaa
      601 cagcacaata tccgacttgt tgtgcccgcg atccgcctgc tccttcggcg cattgcgcag
      661 cacgtctttg tccccgacac ggggtttcgg ttcgagcgga cgcacgtctg gaactttttc
      721 tgtcggctgc atgtttcgcc tttatgctgc cgctagatac ctattaatcc tacaggatgc
      781 tcaaaaaggc cttgtttcca cccggcccac cccgaagcgc cgagacgctt cgatccgcga
      841 acggccgcag cgagcgaaga ggcgcaggcg tactttttat cgtacgttgg gcctctaagc
      901 ggcgcgagaa caagcttgga gaagggcgcg tctcggcgcg ccggggcgtg ggtgggtgag
      961 aaaggcgact ttttcagcat catgtcagta gcccagttcg atgagcgcat gctcatcttc
     1021 acgccacgct tcccgcacct tgacccacaa ctgcaaaaac accttcatgc cgaatacccg
     1081 ctccatatcg agccgcgctt ccgtgccgac cgttttcagg cgttcgccgt gtttcccgat
     1141 cacgatcgct ttatgggact ctcgttcaac caggaccgaa gcggtgatgc gtgcgagttt
     1201 gccttcttcg atgaactcgt cgatttctac ggctaacgaa tagggtattt cttcatgagt
     1261 ttgccgcaat agcttttccc ggatcatttc ggccgccaag gtccgcatcg tttggtcggt
     1321 gaccgtgtcg tcatcgtaag ccgcctctcc ctcgggaagc agtgacaccg cgacggtcag
     1381 caaccgttgg acattatcgc ccgtctgtgc cgacacgggt acgacttccg tccaggcgaa
     1441 caatctcgcg tagctctcga gcaccgggag cagcttgatc ttgttcacga ggtcgatctt
     1501 gttgagcacg aggaggaccg ggcgccttcg cttgttcacg gcggccgtca catgatccag
     1561 caccagcaga tcaccgggtc ccggcaaact ggtcgcatcc ataaggacaa ataagacatc
     1621 ggcttcttcg agcgtttcca cggccgtccg gaccatgcgc ctattcagga gatgctgcgg
     1681 cttatgaagg cctggcgtat ccagcagggc gatctgcgcg cccggtgcat gcacgacacc
     1741 gagaatcctg gttctcgtgg tctgcggctt ctctgagaca atggcaatct tctcgttcaa
     1801 caagcgattc agcaatgtgg acttgcccac attggatcgt ccgatgatgg ccacggtccc
     1861 gaatttcacg gccgcgatcg ctcctgtccg tcatccggga ccagttgata cacggtttcg
     1921 ccttttcgaa cgtatcccag ccgttcccgg gccaactgtt ctatccgtgc gggatcacgt
     1981 tccaagcgct cgatgtctcc gcgcaacatt ttggtcatcc tctgaaggtc ctggatgtcc
     2041 cgttcgagct cttgagcatg atcgcgcatg gaaaaaaatc gcgggagacc catctcacca
     2101 aagcacaagg ccaggagtaa cagcaggccc ataccggcgg cgacgtaatg ggcgccagtc
     2161 accatccgac gttgccagtc caaccatgct cgcccgcgat tgcgcttgac gatcatacgt
     2221 gcctatcgac tgcgaccagg catcgcctcg cgtccacgat agacggctgc ggcgcccaat
     2281 tcttcctcga ttcgaagaag ctggttgtac tttgccaccc gatcggtgcg ggacagagag
     2341 cctgtcttga ttaaaccgct gttcgtcgct accgccacat ctgcaatcgt tgtgtcctcg
     2401 gtctctcctg atcgatgcga aataatcgcc gtatagccag accgcttggc cagttcaatc
     2461 gcctctaacg tttcggtcaa tgtgccgatc tgattcaact tgatcaggat cgaattaccg
     2521 atgccttcat cgatgccttt agcgaaaatc tcgacgttgg tcacaaagat atcgtcgccc
     2581 acgagttgga ccctctcacc cagtttctcg gtgagcagtc tccatccctt ccagtcgacc
     2641 tcgctcaagc catcttcgat cgagaggatc ggataacgat ccagcaactt gccataatag
     2701 gcaatcatct cttccgacga gcgctcccgt gttttctccg cctccagcac atagcgattc
     2761 ttgtcataca gctcgctggc tgcgcagtcc aacgccagcg ccatatcctg gcccggttga
     2821 tagcccgctt cctcgatcgc ttccatgatc aacgtgaggg cttcctcatt ggattgaagg
     2881 tccggcgcaa acccgccttc atcgccgacg gccgtactga ggcctttctt tttcaataag
     2941 gccttcaaag aatgaaacac ttcggtcgac atccgaaccg cctcgctgaa atgacttgct
     3001 ccgatcggca cgatcatgaa ctcttgaaga tccagccgat tgtcggcgtg ggctccgccg
     3061 ttgataatat tcatgagggg aaccggaagc acccgcgcat tggttccacc gagatagcga
     3121 tacaacggct ggccggtttc aatggcagcg gccttggcca cagccagcga gacgcccagg
     3181 atcgcattgg ccccgagctt tcccttggtc ttggtaccgt ccaactcgat catggcctgg
     3241 tcgattccga cctgatccaa ggcgtctctt cccaacaatt ccggtgcgat ggtcttgctg
     3301 atgttcccca ccgccttcga cacgcccttg cccatccagc gctttttgtc tccgtcgcgc
     3361 agctcgatcg cttccttttc ccccgttgat gcgccagacg gcaccgccgc ccggcctttc
     3421 gccccgctct ccagtgtcac ttccgcttca acggtgggat tgccccgcga atcgatgacc
     3481 tgcctgccct tgatctctct gatcgtgctc atgctctcgt tctccccctg ctggctcgct
     3541 ccttcatccc aacttccttt tcaataactc attgaccttg cttgggttgg ccttccctcc
     3601 gcttgctttc atcgcctgac cgaccaaata tcccagcact tgctgcttgc cttccttgaa
     3661 ttgggctact tggacaggat tgttcagcag catctcgtcg atgatcgttg aaagcgcctc
     3721 ttcatcggac acctgggtca gaccttgctc ttgcacgatc tgctcgggcg atttttcgct
     3781 gcgatagagc tcagggaaaa tgtcacgcgc gacctttaag ctaatcgtcc ccttctccac
     3841 catctctaat aacgtgacga gacgatgagg agaaaccgga gagcctgcga cggtcgtgcc
     3901 gctattgttc aactctctcg tcaattcacc catcacccag ttgctgaccg tcttcggctg
     3961 attgaacagc tgggtggctg cctcgaaata gtccgccatg tccttggagg ccgtgagcac
     4021 tgcggcgtca tactcgggca gaccgtactg ctcgacaaac cggcgcgcac gtatggcggg
     4081 cagttcaggc agaccgccct gaaacccctc gatccattct cgctcgagct tcaaggggac
     4141 gagatctgga tccgggaaat atcggtagtc atgcgcctct tctttgcttc gcatcaccgc
     4201 cgtctcgccg cgatccaaat tccacaagcg tgtttcctgc tggatcgttc cgccctcgct
     4261 caagaccctt gtctggcgct taatctcata ctcaatggca tccttcacca acttgaacga
     4321 gttgatgttc ttcaactcga ccttggtgcc gaactcttgt tggccgactg gacgcaacga
     4381 caagttgggt tcgcaccgga aactcccctc ttccatgttg ccgtcacaga cttggagata
     4441 catgaggata tcgcgaaggc ttcgcaagta ggcgacgacc tcgtcggccg atcgcatatc
     4501 cggttcggtc acaatctcca acagcggcgt gccggcacga ttgaagtcga cgacgctgac
     4561 gcctgcagcc gcgccatgga tattcttacc ggcatcctct tccaggtggg cgcgtctaat
     4621 ccgcacgcgc ttgtgcgcct cgccggtcgc gatatcaatc cagccgtctt cgcagatagg
     4681 agactcgtat tgggaaattt gatagccctt cgggagatcg ggataaaagt aatgtttccg
     4741 cgcaaatcgg ttgtgctcgc cgatcctgca attcaaggca agacccgccc gcacggccat
     4801 ctcgaccgcg gccttgttga tgaccggaag gctgcctgga agaccgaggc agacggggca
     4861 ggtctgactg ttggcagaca ggccgaacgt cgtcccacat ccgcaaaaca tctttgattg
     4921 cgtccgcaac tgggcatgga cctcgacgcc gatgacgact tcatagccgg tttgactagc
     4981 actctgcgga atcatcgcag ctccatttgc ataagacagg cgtgtggcaa aatatctctc
     5041 acccggcgag cgaaaagaga ggagaagata cgtgtctcga aagaattgtg ggcgccctta
     5101 gctttcgccc ctcgtctgct ctcgcttcat cgcctcccga ccggcttcaa aggcctcacg
     5161 caacaccgac ttcctcgcct cgacgtactc tttgccttca ttgaccgctt cttcaagccg
     5221 ttgcccggcc tcgttggccg cttcgcgcaa ggcgtcctcc gctttgcggg catagccgcg
     5281 tagctgctct cgtgtggtgc gtccggattg tggcgccaac agcaatgcgg caatcgctcc
     5341 aatcgccgcg ccccccagaa acgccaatac tgtcgctgct cccgattccc gttcatgccc
     5401 tcgttcatgc cccattgtat ggttctcctt cctgacgata acgattacgc atcacccggg
     5461 tcgcggcctt aacgcctgcg acaagactcg cgacattcgt caacagcgta ccgctcgagc
     5521 cacgcatgac attatgcact tcctgcaccg actcacctac ctcaccgacc gcatgcagca
     5581 gcacagacgc atgctcgacg ccattccggg cctgatcagt caatgcattc aagttttgcg
     5641 tgacggaacg aagatccgac gacagcgcag gaagatccgc attcagcctc gtcatgagcc
     5701 gttcggactc ggcgaccgtt ttgcgcaggt tgatcagcac cggcaccacg aagccgacga
     5761 ggacggcaaa agccgcagca gcgagacaag cagccagttc aatgagcgtc ataccaacct
     5821 ccgggctacg gacgagaagg gtgttccgct tgttttcatc ggacaatcgc tttcttgagg
     5881 tgccactgtg tggactgctc gtaggcatgc gcagccctga gcagcgtctc ctcctcaaag
     5941 gggcgcccga tcaactggac gccgatggga aggcccgcct tgctgaatcc gcatggcact
     6001 gcaatcgccg gcaaccccgc aagattgacc gagatggtgt aaatgtccga caaatacatt
     6061 tgcagaggat cctcgctctt ctcccccagt ttgaacgccg gcgtcggcgt gaccggcgtc
     6121 acaatcagat cgacgtcctt gaaggcggcg cgaaaatcct cgcagatcag cgttctgacc
     6181 gcctgtgctt ttccgtagta ggcatcgtaa tagcccgcgc ttaaggcata ggtgcccagc
     6241 atgatgcggc gttttacttc cggtccgaac ccttcttgtc tggttttcat gtacaggtcg
     6301 aggagatcct tcgtctcctt tgcacgcagg ccgaatttca caccgtcata acgtgccaga
     6361 tttgaactgg cctcggccgt cgcgatcacg taatagaccg caacggcggc atctgtcctg
     6421 ggcaaatgaa tctccttgat ggtgccaccc aggcttttca gttcgtcgat tgccgcacga
     6481 acggacgact cgacttccgg atccaggccg tcggcgaagt attccaccgg cacaccgaca
     6541 ttcaactttt tgagatcttt tttcttcagc gctttcatgt aatcgggaac gggcaggtca
     6601 gcagaggtcg aatccaacgg gtcgtgaccg gcgatggcgc cgagcaagag cgccgcatcc
     6661 ggcacatctt tggtgatcgg accgacctga tccaatgacg aggcaaacgc cacgagacca
     6721 tagcgggaca cccgtccata cgtgggcttc agacccacaa cgccgcagaa tgccgccggc
     6781 tggcggatcg agccgcccgt atctgagccg agcgccgcca cacactcatc cgccgccacg
     6841 gctgctgccg cgcctccgct cgagccgccg ggcacatgct ggagattcca ggggttccgg
     6901 cttggaccaa atgcggagtg ttcggtggat gagcccatgg caaattcgtc gaggttcgcc
     6961 ttgcccagga ggatatagtc ttggtcccgc aatttggcga taaccgttgc gtcatacggt
     7021 gggatgaact gctgcaacat ccgtgaagag caggtcgtcc gcactccttc cgtacagaga
     7081 ttgtccttaa tagccagagg catccccgtc atcggcttgg ttttgcgcca ccccttcaag
     7141 cgctcgtcca gtgcgctggc ctgcgccagg gcggcttcct tggtctgcga cacataggct
     7201 ttcactttgg gctctacctg cccgatccgg aggaaatagg cgcgcacgat ttccgtggcc
     7261 gtgacctcgc cggcagtaaa tttcttctgg agttcacaga gtgagagctt gtgcagagac
     7321 atgactgtta tctcgattcg acgatctgat ttttttcgtt gacgcgcacg atcctcggcg
     7381 catgtttttt aatttcctca tcgccgtaat actcgtaaca aaagatgatg atctggtccc
     7441 ccaccgctgc tttgcgcgcc gtcggtccat tcaacacgat atcgccgctg ccccgacggc
     7501 caccgatcgc atacgtcatg aagcgctcgc cgttgttcag attcgagcag acaatcgcct
     7561 cgtacgggag aatcgcggcc gcctccatca gatcttcgtc aatggttaaa ctaccttcgt
     7621 actcgagatg cgcgccggtc actgtgacgc gatgaatttt tgatcgtaac atttgccgga
     7681 acatcgttac ttcaccttac ctctcatgcg atatcgttac cgatcctcaa taatcttcgg
     7741 caccgcaaat gcgtcccctt ctcgttccgg tgcgttggtc aaggccttgt ccacagcgag
     7801 cgacggacgg acgatgtcct cccggaagac gttcaccgcc ccaagtacag cagcggtggg
     7861 ctcaataccc tttgtgtcgt actctctcag tttttcgacg tgcgtcaaaa tttggctgag
     7921 ttgtttggca aacgcttcct tctcagctgg ggacacctcc agccgcgcca acttcgccac
     7981 cttctcaaca tcctgcagtg tgatcttcat cgctcaatca tttccgtagc gaatgttcaa
     8041 catggccgtc actctcaccc gcccaccccg tcgtgcttag aagcacgctc gtcccggcta
     8101 ttccaccgtc acgctcttgg ctagattcct cggctgatcc acgtccgccc cccgcagcac
     8161 agcaatatga taggcgagca gctgcaaggg aatcgtaaac aagatgggcg acatcaaggg
     8221 atgcgtatcg ggaatggtaa atacggcatc cgccgtcctg cctaattctc tctccccttc
     8281 ggctacgagc gcaatgaccg gtgcccgtcg cgccttgact tccatcaggt tactcacggt
     8341 cttttcatac aggcgatctc gcggtgccaa cacaaccacc ggcatgtccc ggtcaatcag
     8401 cgcgatcggc ccatgtttca tctcgccggc cgcatatccc tccgcatgga tatacgacac
     8461 ttccttcaat ttgagggaac cttccagcgc gatgggatag ttgatgccgc gtccgagaaa
     8521 cagaaagttg cgctttttat aatagcgttt ggcaatcgcg acgatttccg cttcccgtcc
     8581 caacactcct tcgaccagcg aaggcagccg cacgagccgg tccagccatg ccttcccgtc
     8641 cgccacattc atcacattgc ggacgcgggc caaatggagc gccagcagat agagggcggt
     8701 cagttgggcc gtaaatgcct tggtggaggc gacaccgatc tccggcccgc aatgggtata
     8761 gagcacgccg tcagactcgc gggccagcgt gctgccgacg acattgacga tcgacaggac
     8821 gcgagcgcct ttctccttgg cttcccgcgc ggcggcgagt gtgtcggctg tttcgccgga
     8881 ttgggaaatt gtaatgaaga gatcgtgctt cccgacgagc ggatcgcggt accgaaactc
     8941 actgccgatg tcgacttgga ccggcgtccg aaccatttcc tccaacagat atttgccaac
     9001 cagtcccgca tgccacgacg tcccgcaggc cacgatccag attcgatcca cggccagaaa
     9061 ctcgcttggc gtcaggccga tgtcggcgag gtccgcttcg ccggtttcat acgaatagcg
     9121 gcctcgcatc gtatcgagaa tcgtctgagg ctgctcgtgg atctctttca acatgaaatg
     9181 agggtatccg cttttctccg ccgcagaggc gtcccatgtg acgcgtgaag tcttccgcga
     9241 gacctcccgg ccttcggcat ctattacctg cacctcggac tgggtcacgc tcgccacgtc
     9301 gccttcttcg agatagatga cgtctcgggt gtgcgccagc attgccatga catccgatgc
     9361 cacgtaagag gcctgcttcg tgcgccccac gacgagcgga cagccggagc gcgctgcaat
     9421 gagccgctcc ggctcttttt cgcagatcac ggccagcgcg tagctccccc gcacttcctt
     9481 ggtggccgca cggacggcat cggccaggtt caacccgcgt tggagatgtt tatcgatgag
     9541 gtgggccacc acctcggtgt cggtttccga ctggaaggca tagccctctc tttgcaactg
     9601 ctgcttgagc ggttgataat tctctatgat cccattgtgg acgaggacgc atccattcga
     9661 tcgatgcggg tgcgcattct gttccgacgg ctttccgtga gtggcccatc gggtatgtcc
     9721 gatgccaaga acgccattga tggctttttc cgcgagcgac ttttgcagat tggccaattt
     9781 gcccacgctt ctgcggacct cgattttctc accctgcaag acggccacgc cggcggagtc
     9841 atagccccga tattcaagct tggctaaccc ggcgatcaga atcgggaccg cttcctggct
     9901 gccgacatat cccacgatac cgcacatagt acgaactccg tcttatcgtg atcgttttgc
     9961 tgacggcttg accgtccgct ttcgcgacgc ggccttgcgg gattttccgc tggactgttt
    10021 ccgattggag accggagcaa cgcccgcacc aaacagcgct cgccgcttgg cggcccaccc
    10081 gggtcttgtg acctgacggc cccgcgagat cacgagggaa tccttgggga cattttccgt
    10141 gaccgtcgtt ccggccgcaa tcacggaccc ttcgcccacc gtcacaggcg cgaccaattg
    10201 cgtgtcactg ccgatgaaca ccccgtcttg gacgatcgtt tcggcttttc gcattccatc
    10261 ataattacag gtgatggtgc cggcgccgat gttcacgcct ttgccgattt tcgcatcccc
    10321 aaggtagctg agatgattgg ccttcgaccc ttctcccaat tcggtttttt tgacttcgac
    10381 gaaatttcca acctttgcgc ctttgcgcac gacgacatga ggacggaggt gggtaaatgg
    10441 ccccagcgtc gcatcgtctt caatctctga atcgcaaaac acgcagtggt cgagaatctc
    10501 gacgcgatcg ccgatgttgc aattggtgat tcggcagccc gatcgaacaa tactatcctc
    10561 gccgatcacc gtcagccctt ccaacatgac gttcggatag agcaccgagt cttttccgat
    10621 cctcacctct gcgtcaatcc atgtcgaggc gggatcgatc atccgcactc cggcgtcgag
    10681 ccaacgatca cgaatctgcg ctcggacgac ttgctcggcc tcggccaact gccgtctggt
    10741 gttgacaccg agaccttcat gcgggtcttc gaggagaaca gccgcgaccg ggcgcccttc
    10801 ttcaaccgcc atgtgcacga tgtcggtgag ataaaattcg ccctgcgcgt tggtgggctg
    10861 gattttttcg agagcggaaa acaagaaatc tccttcgacg acatacgtcc cgacgttaat
    10921 ctcatggatg gctttggtcg ccacgtcggc atcacgctct tccacgatcc gcgagacata
    10981 gtcggtcgaa gcctgtctca ccacgcggcc atagccgctg gcatcgtcga ggacggccgt
    11041 cagaatggta acggcggcct gttgctgctg atgggcgtcg agcagtcgcc gcagagtcgt
    11101 ttccttgagc aacggagtat cgccattgag aatgagaaag cgcccgggtg tggaccgtcc
    11161 agactcgaga aacacgggcc gcgtctgtaa gacggcatgg ccggttccca acagttcccg
    11221 ttgttcgacg attgaaaccg gtgcgtgtcc gcttctttct cctgtggcct gcttgatgac
    11281 ttggctgacg cgatcggcct gatggcccac gacgatggcg acgtgatggc cggcgacacg
    11341 tagagccagc tgaaccgagt acaagaccat cgcctgcccc gcgaccggat gcagcacctt
    11401 ggcctgcttg gaatgcatcc gttttcccaa gccggcagcc atgactatcg ccgccaggtc
    11461 tggaacggga cggtcggccg tctctcgagg ctcctgatgt ctggtggcgt cctgacgcga
    11521 tagccggctc atccgatcgg cactcccgct tccggctgct tgacggcccc ccatttggct
    11581 tccggatcta acgagatgcc cagtgaggag gatggtgaat agagtccacc tgacacgatc
    11641 agcttcatcg cttcttcaac actgatgttg accgcggtga cctcccgttc gggaaccag
//
LOCUS       DASSCQ010000187         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_34049, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000187 DASSCQ010000000
VERSION     DASSCQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34049"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFH05_12230"
     CDS             complement(<1..310)
                     /locus_tag="VFH05_12230"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET6679033.1"
                     /translation="MTRVSGKPIEIRVNQPTAGSDEPTFPPYNGHAFFSYGFRPFFLG
                     AAVFAGAAVPLWILILAGAIGSGFLYAPREWHVHEMLFGFLPAVMTGFLLTAIPNWTG
                     R"
     gene            complement(605..1261)
                     /locus_tag="VFH05_12235"
     CDS             complement(605..1261)
                     /locus_tag="VFH05_12235"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6679034.1"
                     /translation="MNSRKRATFLFFDRSMLLVMVLWLAPVSPALAALPQTPTESVRS
                     TIEDVIRILTNEQLKQPDRLAERRQEIERIVRDRVSYEDMAKQALGLPWLNLEAEERQ
                     EFVDLFVQLLRDSFAAKIDAYADEHVLYLSELRDDHMAEVRTKLAGRKTDSLLNFRLA
                     DRAGTWLVYDVVIDGASIVHNYRAQFARIIRDVSYPGLVELMREKTLVVKAFEAGHTE
                     "
     gene            1598..3667
                     /locus_tag="VFH05_12240"
     CDS             1598..3667
                     /locus_tag="VFH05_12240"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET6679035.1"
                     /translation="MAALSYHHPAVKVTGWVLIACAYFFAGKIGLGFASVNVSASPVW
                     APAGMAVALFLLLGFSIWPAILAGAFLVNVTTAGSVLTSANIAVGNTLEGMLGAWFVQ
                     RFAHGIHAFESPPDILKTALLAGALAPTVSATIGVTSLTMAHFASPHDYADIWLTWWL
                     GDATGILIVTPAILLWARNPRIEWTSRERLELALLLLVLVVTCTVMFGPFINTGDRYS
                     FRVLLVPILIWAAFRFGPRETATLAVLLAGWVTWGTLNGRGLFTGDSENEAFLFGQGF
                     LGVNTVMALALAAAVQERKEAEVRFQDTAERLVQKRTEELLHTQARLRTMAQALTLTE
                     QRERKRMAGELHDYLAQLLVLSKMKLSRVHAQIQTIGGPVAAVVEEIDHTLAKALDYT
                     RTLIAELSPPVLHELGLPMALQWLADQMKKHQLNVDVQLGTSQLPLTENQAILLFHSA
                     RELLINAAKHAGTNKATLTLQAADNMLTIAVQDRGKGFQTDKIEPQQLGQHFGLFSIK
                     ERMEELGGWLRVESVPGVGTTVTLGLPLTPAALSPAPLGNEGQVHPPAKTAGVRRVLL
                     VDDHAMVRQGLRAVLDAYPDLFIIGEAADGREAVSIAKKRIPDVIIMDINMPRMDGIE
                     ATRRIKKEQPGTVIIAVSVNDTPDIRESLQKAGASAFVSKDEAGERLYETIMTMALPQ
                     QEATGLS"
     gene            3681..4295
                     /locus_tag="VFH05_12245"
     CDS             3681..4295
                     /locus_tag="VFH05_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679036.1"
                     /translation="MRSLFVLHSALLLLILVSPSPSYDQAFRLGSEGEWTASQQRHAP
                     FHTVLEKNDTRPDPSKELLRDGEYGPAREIKRFTGPTIQLRGTAEEIGLDSRALASGL
                     EMKFLQDFAFLQGNFAFDKTYETWEIGLFDCEAWTVGLTYPIAFHLQCVAGSMDEPRH
                     WRYATLGYGPKEKIVETIRKALDSIIGEYASFIRKAHSKKDGGT"
BASE COUNT         1006 a         1293 c         1331 g          911 t
ORIGIN      
        1 gtcgtcccgt ccagtttgga atcgcggtca gaagaaagcc ggtcatcacg gctggcagga
       61 atccaaacag catttcgtgg acgtgccact cgcgcggagc ataaaggaaa ccggaaccaa
      121 tcgcgccggc caggatcaag atccagagag gcacggcagc tccggcgaac accgcggcgc
      181 cgagaaagaa tggcctgaag ccataggaga aaaacgcgtg gccattgtaa ggagggaagg
      241 tcggttcatc cgacccggct gtcggctggt taaccctgat ctcgatcggc ttgcctgaca
      301 cgcgagtcat tctcgagaac cggccaaagg gttgtcaact ccgcggaagg aaatcactgc
      361 atcccttttc agagaagaaa acgcgccaat gaggatcagg gcgtcgcaag caggcggacc
      421 tcacgctgag ggaagggaac ctcaatgcgt gccgagcgaa atcgctcgat gatggcctgg
      481 ttcagctccc tctccacctt cacgaaattg acgaccgcta cccacggatc acaaaacacg
      541 aagaaagtaa gcggtgagcg gcttggtcgt cggtgagcgt ctcgagaacg tcgcctgtgt
      601 ttctttactc ggtgtgcccc gcttcgaagg ccttgaccac gagcgttttc tctcgcatga
      661 gctccaccag ccccgggtac gagacatcgc ggatgatgcg cgcgaactgc gcgcgatagt
      721 tgtgcacgat gctcgcaccg tcgatcacga cgtcatacac gagccacgtg ccggcccgat
      781 cggccagccg gaagtttaag aggctgtcgg ttttgcggcc ggcgagcttc gtccggacct
      841 cggccatgtg gtcgtcccgc agttcggaga gatacagcac gtgctcatct gcgtaggcat
      901 cgatcttcgc ggcaaacgaa tcccggagca gttgaacaaa cagatcgacg aattcctgac
      961 gctcttccgc ctcgaggttc agccatggga ggcccaacgc ctgtttcgcc atatcttcat
     1021 agctgacccg atctcgcacg atccgttcga tctcctgacg ccgctcggcc agacggtcag
     1081 gctgtttcaa ctgctcgttc gtgaggatac gaatcacgtc ctcgatggtg ctgcgcaccg
     1141 attccgtcgg cgtttggggc aatgcggcga gagccggcga taccggcgca agccacagca
     1201 ccatgaccag aagcatgctc cggtcaaaga aaagaaatgt cgctcgtttc ctcgaattca
     1261 tgggcgcctc cggtaagacg tcatccggtt cgccggtgcg agagcgcaag atcagtgccg
     1321 gaagggccat gaatcgtgta ctgaaaagaa tctgaaaagc gtcgcaaatc cgcgatgcta
     1381 tcggccacgg acgaatgccg ggccaaaagg ctcagagcga aaaaggcttc aggtgtggta
     1441 gcaaacactc acgcttttct ctccccatgc gccaatgttc acacccaaaa gaacgccttc
     1501 gttgaggatg attgagccgc gccactgtac tgccaatttg gtctagatcg tagcgctgcc
     1561 ttcctttata gtgagtgttt cgtcacccca catcaacatg gcagctctgt cctatcatca
     1621 tccggccgtg aaagtgacgg gctgggtgct catcgcctgc gcatatttct tcgccgggaa
     1681 aatcgggctt ggcttcgcgt cggtgaatgt cagtgcgtcg ccggtatggg ctccggccgg
     1741 catggcggtc gccctcttcc tgctcttggg gttctccatt tggccggcaa tcttagccgg
     1801 ggcgtttctg gtcaatgtca cgacagctgg atccgtcctc acgtccgcga acatcgcagt
     1861 cggaaataca ctggaaggga tgcttggcgc gtggttcgtg caacgctttg ctcatggcat
     1921 tcatgcgttt gaatctccgc cggatattct gaagacggcg ctgcttgctg gagcgctcgc
     1981 gccgaccgtg agcgccacca ttggcgtcac gagcctgacg atggcgcact tcgcgtcccc
     2041 gcatgactat gcagacattt ggctcacctg gtggctcggc gatgcgaccg ggattctgat
     2101 tgtcacaccg gcaatactat tgtgggcccg caatccccgc atcgagtgga ccagccggga
     2161 gcgactcgag ctcgccctct tattactcgt gctggtggtg acctgtacgg tgatgtttgg
     2221 tcctttcatc aatacgggag acaggtattc ctttcgggtc ctgctcgttc ccatcctgat
     2281 atgggcggcg ttccgcttcg gtccccgaga gaccgccaca ctcgccgtgc tcctggctgg
     2341 gtgggtcacc tggggtaccc tcaacggccg agggctcttc accggcgact ccgaaaatga
     2401 ggcgttctta ttcgggcaag gctttctcgg ggtgaatacg gtcatggcct tggcactcgc
     2461 cgcggccgtg caggaacgca aggaagctga ggtgcgattc caggacactg ctgaacggtt
     2521 ggtgcagaag cgtaccgaag agttgctgca cacccaggcc cggttgcgca ccatggccca
     2581 ggctctcacc ttgaccgaac agcgcgagcg caaacgcatg gccggcgaac tgcacgatta
     2641 tctcgcccaa ctgctcgtgc tcagcaaaat gaagttgtcg cgagtccatg cacaaatcca
     2701 gaccattggc ggtccggtgg ctgccgtggt cgaagagatc gaccacacac tggccaaagc
     2761 actcgactac acgcgcacgc tgatcgcgga attgagcccc ccggtcttgc acgagttggg
     2821 tctgccgatg gcgctccaat ggctggcgga tcagatgaag aaacatcagt tgaacgtgga
     2881 cgtccagctg ggaacatccc agctcccatt aacggaaaat caggcgatct tattgtttca
     2941 ttctgcccgg gagctcctca tcaatgccgc gaaacatgcc ggcacaaaca aggcaacctt
     3001 gacactccag gcggcggaca acatgttgac cattgcggtc caggaccggg gaaaaggatt
     3061 ccagaccgac aagattgagc cgcaacagct ggggcagcat tttggactct tcagcataaa
     3121 agagcgcatg gaggaactgg gaggatggct gcgagtcgag agcgtgccag gtgtgggcac
     3181 gaccgttacg ttgggactgc cattgacacc ggcggcgtta tccccggccc ctttggggaa
     3241 tgaaggacag gtgcatccac cggcaaagac ggccggcgtg cggagggtgt tactggtgga
     3301 cgatcatgcc atggtccgcc aaggacttcg agccgtgctg gacgcctatc ctgatctctt
     3361 tatcatcggc gaggcggcag atggccggga agccgtgtcc attgccaaga aacggatacc
     3421 ggacgtcatc atcatggaca tcaatatgcc gcgcatggac gggatcgaag ctacaaggcg
     3481 gatcaagaag gaacaacccg ggacggtcat cattgccgtc tcggtcaatg acacgcctga
     3541 catccgcgaa tcgttgcaaa aagccggcgc ctccgcgttc gtatcgaaag atgaagctgg
     3601 cgaacgactg tacgagacga tcatgacgat ggcacttccg cagcaagagg ccacgggact
     3661 atcctgatgc ggataatcgg atgagatcat tgttcgttct tcatagtgcg ctcctgctcc
     3721 tgattctggt ttctccatct ccaagctatg atcaagcgtt caggctgggc tctgaaggtg
     3781 aatggacggc atcacaacag cgacatgctc cctttcacac cgtcctcgaa aagaacgaca
     3841 cccgacctga cccctccaag gaactgctgc gagatggtga gtatggacca gcacgcgaga
     3901 taaaaagatt taccgggcca accatccagt tgagagggac ggccgaagag attggactcg
     3961 acagccgcgc actcgcgagt ggtcttgaaa tgaagttcct gcaagatttt gcctttctcc
     4021 aaggcaactt tgccttcgac aagacctacg agacgtggga gatcggccta tttgactgtg
     4081 aagcctggac agtgggcctt acctacccga tagctttcca tcttcaatgt gtagctgggt
     4141 cgatggatga accccggcac tggcgctatg ccacgctggg ctacgggcca aaagagaaaa
     4201 tcgtggagac catcaggaaa gcgttagatt ctattattgg tgaatacgcc tcctttatcc
     4261 gtaaagccca tagcaaaaaa gatgggggca cgtgaggagt ggggggaacg acacgcatat
     4321 cgttcctgtg ctcccggagc gcgcacgcgg gaagaggaaa gacgaaggct gttggcctct
     4381 tatgctcgcc caacgcgcac gcatgatgaa actcattgtt acactttggg cggtgctcgt
     4441 cgaagcgcgc ggtaagaggc caatcagcct tgtcgcggag agcatcagta gcaagcttgg
     4501 aaggaccatc tacgagcgca gtaggagcga tatgcgtgcc g
//
LOCUS       DASSCQ010000188         6981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_320209, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000188 DASSCQ010000000
VERSION     DASSCQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320209"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFH05_12250"
     CDS             complement(<1..535)
                     /locus_tag="VFH05_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6679037.1"
                     /translation="MRQDRVRIFDSQGEAYKQAFQIFLDHTDQKRNAKRWLQEVVNGL
                     PARKVFIDAGAGNGELTKAFADVFTRTIAIEPNVYLLNQLQHSLPTVEVLGLPILAAQ
                     PAAQGDLVLCSHTFFYIPADEWLMHLERLVSWMAPTGVTIVVLQHCESGCMHMVRHFF
                     GHRFELRQVAEAFRAKHG"
     gene            711..1289
                     /gene="pyrE"
                     /locus_tag="VFH05_12255"
     CDS             711..1289
                     /gene="pyrE"
                     /locus_tag="VFH05_12255"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET6679038.1"
                     /translation="MSVLNELARSFHETHSFKWDREKGFTLASGYTSPFYVDCRALMA
                     HPSARRLVGQLAHEALIGVEIDCLGGLEIGAIAIATTISDYAFAASARREWRTFVVRK
                     QAKDHGLGKMIEGAVRSGDRALIVDDVLTSGGSLLKAITAAREAGLTVTDALVIVDRK
                     EQDGRAKVEQCGVKVTSLLTIDDLIAQQSRRS"
     gene            complement(1303..1608)
                     /locus_tag="VFH05_12260"
     CDS             complement(1303..1608)
                     /locus_tag="VFH05_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679039.1"
                     /translation="MKTWSSLACVLVLCAAGCAEGAKLAQSNEDGGVVTYPFKGDQGP
                     MLSSFRKDALALMKDTCSGGYLIVREGEARGRARVVGVEGGQEILRERRWGIEFRCK"
     gene            1669..2082
                     /locus_tag="VFH05_12265"
     CDS             1669..2082
                     /locus_tag="VFH05_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6679040.1"
                     /translation="MIRRIAMTVLGLLLVTGLTTTVTAHHSYLLSVQQLRATLTKAPS
                     MAQKGFVLIDVRSPEEHAAGFIPGTDLNIDFREIKTRHREIGVALDEHVVVYCQSGHR
                     SNIAAETLADLGYTHAYNVRGSMNAWVEAGYPVTR"
     gene            complement(2113..2979)
                     /locus_tag="VFH05_12270"
     CDS             complement(2113..2979)
                     /locus_tag="VFH05_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679041.1"
                     /translation="MKLLNDPLRVRALLIAALSLSGFALEPAWAQTIAWDRPSEGLAI
                     GVWNPSTACPDVPALLVSEIDPVRYRVSVHYFRNEPLPEPPDILEWQRRTGHDLVFNA
                     GLFRENFSYLGLLYGKGKPMGGKRHASWMGLFVAEPTAGGPSRAGILDLSIDSFDEQQ
                     PAYGEAAQSLMLLDRTGKVRVNQTGKVSQQTIVGELRSGHILIMKTTEMTSLHAIGQC
                     LRDAYPTIRQAMAMDGGSSSDLMISPGLRQAVQKTTGTHEWMTLVNSGPTGHVGLPAV
                     IGISPRQQSGRP"
     gene            complement(3033..4649)
                     /locus_tag="VFH05_12275"
     CDS             complement(3033..4649)
                     /locus_tag="VFH05_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679042.1"
                     /translation="MKTPLRLLLLEDDPVDAELVAATLSEAGVVCTAQRVDTREAFLK
                     ALREGGYDLILADYSIPGFDGMTALGLARKEAPEIPFLFVSATIGEELAIDAMHQGAT
                     DYVLKQRLGRLVPSVLRAIRERDERRERKKAEDALVQSEKQFRQAQKMEAVGRLAGGI
                     AHDFNNLLTVIMGYSHVLSAELGSEHPLHDKIEETQKAGEKAAMLVRQLLAFSRKQPL
                     EPKHLSVNNVVSNLEEMLRRLIGADIRLVIKLDPSNSQVLADQAQLEQVLMNLVVNAR
                     DAMPHGGTLTIETARTELAKSPLYHVEPLPPGQYVKLSVTDTGSGMDRDTQAHIFEPF
                     FTTKEEGKGSGLGLSTVFGIVTQSGGGIDVNSRVGYGTKFDIFFPRVAADAQAAAGRR
                     AGEQPIGGTETILLVEDDSSVRILLRDSLRKLGYRVIEAKHGIEACLMASQELANLHL
                     LLTDMVMPGMGGRELAQHLITMKPDLRVLFMSGFTDDVGILAGHEQGTSGFLQKPFTP
                     EVLARTVRKILDAASSNASQPTGNRASSSG"
     gene            complement(4699..5142)
                     /locus_tag="VFH05_12280"
     CDS             complement(4699..5142)
                     /locus_tag="VFH05_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679043.1"
                     /translation="MTPVKPILLAEDNPRDAELALAAMEEHHIADKVVVCHDGAEVLD
                     YLYCRGSFKSRQWGNPVVVFLDLKMPKVDGIEVLRTIKNDVNLKPIPVVMLTSSREER
                     DLAQSYALGANAYVVKPVEFQQFISAVKELGVFWGVINEPPPEGS"
     gene            complement(5139..6647)
                     /locus_tag="VFH05_12285"
     CDS             complement(5139..6647)
                     /locus_tag="VFH05_12285"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6679044.1"
                     /translation="MESATQDHPATTPSPPSPVPDPVPTGPAPIAPIRLAEQARRLAT
                     WISHWSIEQRVLAGFGLVFLGILVISVMSYRNTAVVVKNSRLDTASHELIQLLGSIEE
                     ALVAAESGHRRYLVTGDDSYLKAYRGIVEQMPEFSRYLHALAGPSPEQRERVSQLEQL
                     IKQQLDIETNAIRERDTRGAEAVRQLALPGVAKRELDNIHRLIADFETTEQQATGARV
                     LQSAGTTRSTIALLALGGVLQLVLLGSVYYLIHHDITNRRRVAAELRRQGELLQAANK
                     ELEAFSYSVSHDLRAPLRHIDGYAALLSKAAGQALDEKAQRYLQTISDSAKQMGQLID
                     DLLVFSRMGRQEMLRTTVNLNQLIKAVIQDLRLDLQGRTISWKIGTLPDVPGDPAMLR
                     QVFMNLLTNALKFTATRQNAQIEIGAKRDVPGEVTVFVRDNGVGFDMQYVHKLFGVFQ
                     RLHRNDEFEGTGIGLANVRRIVHRHGGRTWAEGVLDQGATFYVALPLKRTSS"
     gene            complement(6768..>6981)
                     /locus_tag="VFH05_12290"
     CDS             complement(6768..>6981)
                     /locus_tag="VFH05_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679045.1"
                     /translation="YITEPHDPSRVTVSGPMKAVIYVGLAGTLIVGIYPQPVIDWVVA
                     ATMMFADLGGQTAGVPDMSSVVASGH"
BASE COUNT         1434 a         2042 c         2013 g         1492 t
ORIGIN      
        1 ctccgtgctt ggcccggaac gcctctgcga cctggcgcag ttcaaaccgg tggccaaaaa
       61 agtgacgcac catgtgcatg cacccgctct cgcaatgttg caacaccact atggtcacgc
      121 cggttggtgc catccaggag acgagacgtt caaggtgcat caaccactcg tccgcaggaa
      181 tgtaaaagaa ggtatgagag cagaggacca gatcgccctg tgcagccggt tgagccgcca
      241 agatcggcaa gccaagcacc tcaaccgtcg gtaggctgtg ttgcagctgg ttcagaagat
      301 agacgtttgg ctcgatggca atggtgcgcg taaagacatc ggcaaacgcc ttggtgagct
      361 ctccattccc cgcgcctgcg tcgatgaaga ctttccgtgc cggtaatcca ttgaccacct
      421 cttgcagcca acgtttggcg ttccgttttt ggtccgtgtg atcaaggaat atttgaaagg
      481 cctgcttata ggcttctcct tgagaatcaa aaatgcggac gcggtcttgc ctcatgcggt
      541 acgcccctta agcaggaaaa gttggcaacg gcgcggttac tatacgctct tttgtctccc
      601 cattcactcg ccttcagacg gacaatgtct ccttaggctg gaccatgctt atgcatgtgg
      661 tgtacagtcc atcgccgttc taagggctcg aacctgcagg agtgagcatc atgtcggttt
      721 tgaacgaact ggcccgatcc tttcacgaga cccactcatt caaatgggat cgggaaaaag
      781 gatttacgct cgcatccggc tacaccagtc cgttttacgt ggattgccgt gccctgatgg
      841 cgcatccgtc cgcacgccgg ctggtcggcc agctggcaca tgaggcactg attggagtgg
      901 agatcgattg tctgggaggg cttgagatcg gcgccatcgc cattgccacg acgatctcgg
      961 attatgcctt tgcagccagt gcacgaagag aatggcggac gttcgtggtc cgcaagcagg
     1021 cgaaggatca tgggctggga aagatgatcg aaggtgccgt caggtcaggc gatcgcgccc
     1081 tcatcgtcga tgatgtatta acgagcggcg gctcgttgct gaaagcgatc accgcagcac
     1141 gggaggcggg gttgacggtt accgatgcac tggtgatcgt cgatcggaaa gagcaggatg
     1201 gtcgggccaa ggtggagcaa tgcggcgtga aggtcaccag tttgctgaca atcgacgacc
     1261 tgatcgctca gcaaagccgc aggagctgac atcgaatttg tcctacttgc agcggaactc
     1321 gatgccccat cgacgctccc tgaggatctc ctgtcctccc tcgactccga ccacgcgcgc
     1381 tcgtcctctc gcttctcctt cccgcacaat cagatagcct ccgctacacg tgtctttcat
     1441 caacgcaaga gcgtctttgc gaaatgacga cagcataggg ccctgatcgc ctttgaaggg
     1501 ataagtcacg acgcccccgt cttcgttgga ttgggctaac tttgccccct ctgcgcatcc
     1561 ggctgcgcag agcacaagga cacatgcgag acttgaccag gtcttcattc ggctcctatg
     1621 ataatcatca cgattcccct tcagacacgc tgcatgtagg aggtcaccat gattcgtcga
     1681 attgccatga cggtactcgg tctgttgttg gtcaccggcc tgacgacgac ggtcaccgcc
     1741 catcattcgt atttgctgtc ggttcaacaa ctgcgcgcga ccctgaccaa agcaccgtcg
     1801 atggcccaaa agggcttcgt gctgatcgac gtccgttcac cagaggagca tgccgcggga
     1861 tttatacccg gcactgatct caacattgac tttcgcgaga tcaagacgcg ccatcgcgaa
     1921 atcggcgtgg ctctggatga acacgtcgtc gtctactgtc agtccggtca ccggagcaac
     1981 atcgcggcag agacgttggc ggaccttggc tatacccatg cgtataacgt ccggggaagc
     2041 atgaatgcct gggtcgaggc cgggtatccg gtcactcgct agtacatctg ccaacagcgc
     2101 cgttcatcga gctcacggtc gtccgctctg ctgacgcggg ctgatgccga tcacggccgg
     2161 caggccgaca tggcccgtgg gtccggagtt caccagcgtc atccactcgt gagttcctgt
     2221 cgttttctgt acggcctggc gaaggccggg actgatcatc aggtccgaag aagaccctcc
     2281 atccattgcc atcgcttgac ggatggtcgg gtatgcatca cgcaggcatt gtccgatcgc
     2341 atgaagcgag gtcatttctg tggtcttcat gatgagaata tgcccgctcc gcagttcccc
     2401 aacaatggtt tgttgagaga ctttgcctgt ctgattcacc cgtaccttcc ccgtccggtc
     2461 cagaagcatg agggactggg ccgcctcccc gtacgcgggc tgctgctcat cgaacgaatc
     2521 gatcgacaag tccaggattc cggccctcga tgggccgccc gccgtcggtt ccgccacgaa
     2581 tagtcccatc catgacgcat ggcgcttgcc tcccatcggc ttgccctttc catacaagag
     2641 tccgagatag gaaaaattct cgcggaagag gccggcattg aagaccaaat catgaccggt
     2701 ccttctctgc cactcgagaa tatccggcgg ttcggggagc ggttcgttcc gaaagtagtg
     2761 gacagagacc cgatagcgaa caggatcgat ctccgaaacc agcagcgccg gcacgtcagg
     2821 acatgccgtg gatggattcc agacgccgat ggccaatcct tccgatgggc ggtcccaggc
     2881 aatggtctga gcccatgccg gctccagtgc gaaacctgac agagagaggg cggcaatcag
     2941 cagtgcgcga acgcggagcg ggtcattcaa gagtttcatg ggcttcaacg gagtataggg
     3001 atggtccgat tcagcgtcaa actcgacggc ggttatcccg agctggaggc gcgattgcca
     3061 gtcggttggg aggcgttgct cgaagcggcg tcaagaatct ttctcaccgt gcgcgccaag
     3121 acttccggcg taaacggttt ttgcaggaac ccactggtgc cttgctcgtg accggccagg
     3181 atgccgacgt cgtcggtaaa gcctgacatg aagagcacgc ggagatccgg tttcatggtg
     3241 attaggtgtt gggcgagctc ccttccgccc atccctggca tcaccatatc ggtgagcagc
     3301 agatgcaggt tcgcgagttc ctgcgaggcc attaggcagg cttcgatccc gtgcttggcc
     3361 tcgatgaccc gatagccgag ctttctcagg ctatcacgca ggagaatgcg cacactcgag
     3421 tcgtcttcga ccaacaagat ggtttctgtc ccgccgatag gctgttcgcc ggcgcgccgg
     3481 ccggccgccg cctgcgcatc cgcggcgact ctcggaaaaa aaatgtcaaa cttggtccca
     3541 taccctaccc ggctgttgac atcgatgccg ccaccggact gagtcacgat cccgaacacc
     3601 gtggaaagac cgagcccgct tcccttccct tcctccttcg tcgtgaagaa gggctcgaag
     3661 atatgggctt gagtgtcacg gtccatccca ctgccggtgt ctgtgaccga cagtttgacg
     3721 tactggccgg gcggcagagg ttcaacatgg taaagcggac ttttggccag ctccgtccgg
     3781 gccgtttcaa tggtcagtgt gccgccgtgc ggcatcgcat ctcgtgcgtt cacgacgagg
     3841 ttcatgagca cttgttccag ctgggcttga tcggcaagga cctgactgtt ggaaggatcg
     3901 agcttgatga cgagacgaat atcggcgccg atcaagcgtc gcagcatctc ttcgagattg
     3961 gataccacat tgttgacgct gagatgcttg ggttctagcg gctgcttgcg gctgaaggcg
     4021 agcagctgtc ggacgagcat ggcagccttc tcgcctgctt tctgcgtttc ttcgatcttg
     4081 tcgtgcagag ggtgctcgct ccccaattct gccgagagaa cgtggctgta tcccatgatc
     4141 actgttaaga gattgttgaa atcgtgtgcg atccctcctg cgagccgtcc caccgcttcc
     4201 attttttggg cttgccggaa ctgtttttcg ctttgaacca gcgcgtcctc ggcttttttt
     4261 cgttcccgtc gctcgtcgcg ctcccgaatc gctcgcagca cggaaggaac gagccgtccc
     4321 agccgctgct tcagtacata atccgtggca ccttgatgca tggcatcgat ggccaattct
     4381 tcgccgatgg tcgccgagac aaagaggaaa gggatctccg gagcctcctt ccgggccagc
     4441 ccgagcgcgg tcatcccgtc aaagccggga attgagtagt cggcaaggat gaggtcatac
     4501 ccaccctccc gcaatgcctt caggaatgcc tccctcgtgt ccacgcgctg cgcggtgcag
     4561 acaacgcccg cttcggacag ggtggcggca acaagctcgg catcgacggg atcgtcctca
     4621 agaagcagca gacggagcgg ggttttcaca ggtcctctcg ggcaggcttc aaagatggtg
     4681 gcgttgtgag cagcgaggct agctcccctc ggggggtggt tcgttgatca ctccccagaa
     4741 gacaccgagc tccttgacgg ctgaaataaa ctgctggaac tcgaccggtt tgaccacata
     4801 ggcgttggcg ccaagcgcat agctttgcgc gagatcgcgt tcttcacgcg acgacgtcag
     4861 catgaccacc gggatcggct tgagattcac gtcattcttg atcgttctca agacctcgat
     4921 gccgtcaact ttcggcatct tgagatcaag aaacacgacg acgggattgc cccattgccg
     4981 gctcttaaag gatcctcgac agtagagata atccagtacc tctgcgccgt catgacacac
     5041 gaccactttg tcggcaatat gatgctcctc catcgcggcg agcgccaatt cggcatcccg
     5101 gggattgtct tccgccagca ggatcggttt cacaggggtc atgaggacgt ccgtttcaag
     5161 ggaagggcca cataaaaggt cgccccctga tcaaggacgc cttcggccca cgtccggccg
     5221 ccgtggcgat gcacgatgcg cctgacattc gccagcccga ttcctgttcc ctcgaattca
     5281 tcgtttcgat ggaggcgctg aaatacgccg aataacttat ggacgtattg catatcgaag
     5341 cctactccgt tatcgcggac aaacaccgtc acttcgccag ggacatcccg cttcgccccg
     5401 atttcgattt gagcgttctg cctcgtcgcg gtgaatttga gggcattcgt cagcaagttc
     5461 ataaatacct gtcgcaacat ggccgggtca cccggtacgt ccggcagcgt gccaattttc
     5521 caagagatcg tccttccttg caagtcaagt cgtaaatctt gaatcacggc cttaatcagc
     5581 tggttgagat tgacggtcgt ccgcaacatt tcctggcggc ccatccggga gaatacaagg
     5641 agatcgtcaa tcaactgacc catctgtttc gcggagtcgg agatggtttg caggtagcgc
     5701 tgggccttct cgtccaaggc ttggccggcc gcttttgaca acagcgccgc atacccgtcg
     5761 atatgccgca gcggcgcgcg aagatcgtgg gacaccgaat aactgaaggc ttccaactcc
     5821 ttgttcgccg cctgaagcag ctcaccctgg cgtcgcaatt cggccgcgac gcggcgccga
     5881 ttcgtaatgt cgtgatgaat gagatagtac acggagccca ggagaacgag ttgcaacacg
     5941 ccgccgagtg cgagcagtgc aatggtactg cgggtagtcc ccgccgactg cagcactcgg
     6001 gcaccggtcg cttgctgctc cgtcgtttcg aaatccgcga tgaggcgatg gatattgtcg
     6061 agttcgcgtt ttgcgacacc gggcaatgcc agctgccgga cggcctcagc gccccgggtg
     6121 tcccgctccc tgatggcatt cgtttcaatg tccagttgct gcttgatcag ctgttcgagc
     6181 tgagagacgc gctcccgctg ctcaggcgac ggtccggcca gggcatgtag ataccgcgag
     6241 aattcaggca tctgctccac gattccgcga taggccttga ggtaggaatc gtcgccggtg
     6301 accaggtagc ggcgatgacc gctttcggcc gcgacgagcg cctcctcaat ggagccgagc
     6361 aattgaatga gttcatgact ggctgtatcc agccggctgt tcttcacgac gacggcggta
     6421 ttgcgatacg acatcacgct gatgacgagt atccccagga agaccagtcc gaatccggcc
     6481 agaacgcgct gttcaatcga ccaatgactg atccatgtcg ccaatcgtct ggcttgttcg
     6541 gcgagacgga tgggcgcgat cggtgccggt ccggtcggta ccgggtcggg gacgggggaa
     6601 gggggtgacg gcgtagttgc cggatggtcc tgagtggcgg attccatgag ttcgtggcag
     6661 cacgaaggcc gaaataggat gaaaacctaa caaactctac tctggccgat ccggccctgc
     6721 aagcaatatt tcagatagct aactaacagg ggaggagtaa agacagatca gtggcccgag
     6781 gcaacgaccg aggacatatc cggcactccg gctgtttgac ctcccaaatc agcgaacatc
     6841 atcgtggcgg ccacaaccca gtcgatcacg ggttgaggat aaataccgac gatcagcgtt
     6901 cctgccaatc cgacatagat cacagctttc atgggacctg agacggtcac ccgggatgga
     6961 tcgtgaggct cggttatgta c
//
LOCUS       DASSCQ010000189         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1409827, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000189 DASSCQ010000000
VERSION     DASSCQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409827"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFH05_12295"
     CDS             complement(<1..391)
                     /locus_tag="VFH05_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET6679046.1"
                     /translation="MAESMHFATTVKRWVFGLAAACLLALGGYALLAKSGEEPSRAGQ
                     MPGPARAMPVQVAAARIGDIGIYLNGLGSVAPLNTVNVKSRVDGQLMKVLFQEGQLVK
                     SGDLLAQIDPRPFEVQLIQAEGQMVRDL"
     gene            complement(415..837)
                     /locus_tag="VFH05_12300"
     CDS             complement(415..837)
                     /locus_tag="VFH05_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679047.1"
                     /translation="MRSRRKLWAGLIILFGAGTLTGIVAGYVYSHTERTTRSEHGPAA
                     HHERIMKRLTQDLSLTEQQQAEIEPIVTRAHVAVLELRFAHQAEVEQILAKGMTEIKT
                     KLSASQQPGLDKLYQELEQRWQASRHYLATQKQTLTSQ"
     gene            complement(853..1167)
                     /locus_tag="VFH05_12305"
     CDS             complement(853..1167)
                     /locus_tag="VFH05_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679048.1"
                     /translation="MNQHTDVDVHKLERALVDSYRSRSGASYEVDVTQQVMRDVRRLD
                     SEREGWTPPAMLDQLIWQTATRAAAIVLIAAMLTVGLFRLQSDRPALVAEDFEPAPLF
                     EE"
     gene            complement(1164..1835)
                     /locus_tag="VFH05_12310"
     CDS             complement(1164..1835)
                     /locus_tag="VFH05_12310"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6679049.1"
                     /translation="MMSHVAFAVDMQALSGWHRSMTDDSLPVVKPRQDERELLRRIRQ
                     GEIERYADLIALHQRHVIRIVGRRVPSDRVAEIVHDVFVRAYVNLGQFSESVPFDHWL
                     TGIAIRTCYDFWRDHARHAVPVSSLTEEHHRWIEQTLSAQSDAQFRRQAETRDAAEVL
                     EWALKQLSPENRAVLTLVHLDGYSVREAAQLLGWTLVNVKVRAHRARRALRNLLRHTS
                     PRSAS"
     gene            2113..2844
                     /locus_tag="VFH05_12315"
     CDS             2113..2844
                     /locus_tag="VFH05_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HET6679050.1"
                     /translation="MSDDLTMARLLSRRDALRLLGGCVACVIAGSPVSLSAIGGPSPA
                     CVFRPEQTEGPYFVDERLNRSDIRSDPMTGQVKSGTPLGLTVRLSRVEKGQCHPLPGA
                     QVDIWHCDARGVYSDVQDAGFSTIGQKFLRGYQISDPQGEARFLTIYPGWYPGRTVHI
                     HLKVRTAPETKQRFELTTQMYFDDALSDRVFADPPYAEKGSRAGRNQDDRIFRRGGEQ
                     LLLSLMPAAEGFSATFSLGLQLPSS"
     gene            2871..4151
                     /locus_tag="VFH05_12320"
     CDS             2871..4151
                     /locus_tag="VFH05_12320"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6679051.1"
                     /translation="MDQKRIVIIGGGFGGLTAARSLRRADVVLIDRTNHHLFQPLLYQ
                     VATAALSPSDIAWPLRTVFRSQRNVRVMMDEVLSIDRADRSICLRESPPVSFDVLIVA
                     PGSRHAYFGHDEWEEFAPGLKTMTDAVRLRQHMLSAFEDVERRLASTGKHEPLTFVIV
                     GGGPTGVELAGALAEIGRKAMGPDFPNLRLDDLCVLLVEGGPRILPGFSPDLSDKAAA
                     ALSRMGVTIRLNSLVSAVDKGGVKIGQDFVPSAGIIWAAGNRASGLLDTLKAARDGAG
                     RVMVNPDLTVPGDPWIFVIGDAAHCRGQDGKALPGLAPVAMQQGRYVAQLIEEGAAPN
                     TRPPFIYADRGMLATIGRAKAVAQIGRFHVSGLLAWLLWCIVHIFFLIGVRSRLRVMS
                     EWIWYYVTFKPGARLLFEQPEPAHRESRGSHRTG"
     gene            4269..4691
                     /locus_tag="VFH05_12325"
     CDS             4269..4691
                     /locus_tag="VFH05_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6679052.1"
                     /translation="MKPRITIVTIGVDDLDRSLRFYRDGLGFPTRGIVGTEYEHGAVA
                     FFDLTNGQKLAIWARRDLAHEAKVPLGPRSASEFSLGHNVDSKAEVDAVISQAQRAGA
                     MITDPPHEAFWGGYTGSFQDPDGHLWEVAWNPEMNPPE"
BASE COUNT          945 a         1433 c         1429 g         1053 t
ORIGIN      
        1 ccaggtcgcg caccatctgg ccctcggcct ggattaactg cacctcgaag ggccgcgggt
       61 caatctgcgc caacaggtcg ccgcttttga cgagttggcc ttcctgaaac agcactttca
      121 tcaactgacc atccacccgg cttttcacat taacggtgtt caagggtgcg accgagccga
      181 gcccattcag ataaatgccg atatcgccga tgcgtgccgc ggccacctgc accggcatcg
      241 cgcgcgcggg gccgggcatc tggccggcac gggatggttc ttcgcccgat ttagctaaca
      301 gggcgtaccc ccccagggcg agcaaacagg cggcggccag cccgaacacc catcgcttaa
      361 cggtggtcgc aaagtgcatc gattcagcca tggtctattt ccagttcact cagattattg
      421 cgatgtcagc gtctgtttct gggttgccag atagtggcgt gaggcctgcc accgctgctc
      481 caattcctga tacagcttgt ccaggcctgg ctgctgcgac gccgacagct tcgtcttaat
      541 ctccgtcatt cccttggcca agatctgttc gacttcagct tgatgggcaa accgtaactc
      601 gagaactgcc acgtgggccc gggtgacgat tggctcgatc tcggcctgtt gctgttcggt
      661 tagcgacagg tcctgtgtca ggcgtttcat gatccgttcg tgatgagcgg caggtccgtg
      721 ctcgcttcgg gtagtccgtt cggtatggct atacacataa cccgcgacaa tcccggtcag
      781 cgttcctgcg ccgaacagga tgatgaggcc cgcccataat ttgcgtcgag atctcatgga
      841 tcctcctcgc tatcactctt cgaataacgg agccggctcg aagtcttcgg ctacgagagc
      901 cggacggtcc gactgtaacc gaaacaagcc gacggtcaac atggccgcga tcagcacgat
      961 cgccgcggca cgcgtggccg tttgccagat caactgatca agcatggccg gcggcgtcca
     1021 tccctctcgt tcagagtcca gtcgacggac atcgcgcatg acttgctgag tgacgtcgac
     1081 ctcataggac gcaccagacc gtgagcgata gctgtcgacg agggcacgct cgagtttatg
     1141 aacgtcaacg tcagtatgct gattcatgac gcactcctag gcgaggtgtg tcgtaaaagg
     1201 ttgcgcaggg cccgacgggc ccgatgtgct ctcactttca cgttgaccag ggtccacccg
     1261 agcagctgcg cggcctcacg aaccgaataa ccgtcgaggt gcaccagcgt caagacggca
     1321 cgattttccg gagagagctg cttgagcgcc cattccaaga cttcagccgc gtcgcgggtt
     1381 tcagcctgac gccgaaactg tgcgtctgac tgtgcggaaa gtgtctgctc gatccagcgg
     1441 tggtgctctt ctgtcaagga actgacaggc acagcatggc gagcgtggtc tctccaaaag
     1501 tcgtaacagg ttcggatcgc gatgcctgtc aaccaatgat cgaatggaac cgattcagaa
     1561 aactgaccca aattgacata ggctctcacg aagacatcat gaacaatttc cgcgactcga
     1621 tccgacggca ctcgtcgccc gacgatacga atcacatggc gttggtgaag ggcgatcaga
     1681 tcggcatagc gctcgatctc gccttgcctg atgcggcgga gaagctcgcg ttcgtcttgt
     1741 ctaggtttga ccacaggcaa cgagtcgtcg gtcatcgacc gatgccatcc ggacagtgcc
     1801 tgcatgtcga cggcaaacgc aacgtggctc atcataccac cgccctatgg tccaaggtgc
     1861 agctcttgtt ttctgctgtt catgagtcgc ggcgctcgtt gaaaaggtta cagggtataa
     1921 gcggttgaat ttcctgcttg taactggagc tcggtcggac ccctcgttcg ccagcggatt
     1981 ggatcggcaa gacagtcaga ctttatttct tgaaagcgcc tcatcaatct gttcacactt
     2041 ggttcggtgg tggatcgaca gcatccatcc accctgtgtt ggccttatcg ctcgagtcga
     2101 gtcgagggga tcatgtccga tgacctgacc atggcccgcc tcctgtcgcg ccgtgatgca
     2161 ttgcggttgt tgggtggttg tgtggcttgt gtcatcgccg gaagccccgt atctctcagt
     2221 gcgatcggcg gaccaagtcc tgcttgcgtg tttcgcccgg agcagaccga aggcccctat
     2281 ttcgtcgatg agcgcttaaa ccgctccgat atccgttctg atccgatgac tggccaggtc
     2341 aaatccggaa ccccgttagg attaacggtt aggctctccc gcgttgagaa ggggcaatgc
     2401 cacccactgc ccggtgctca ggtcgatatc tggcattgcg atgcgcgggg tgtctactca
     2461 gacgtgcagg atgccggctt ctctacgatc ggacaaaagt ttctgcgcgg gtaccagatt
     2521 tcggatccgc aaggagaagc acggtttctc accatttatc cgggctggta tcctggccgc
     2581 actgtgcaca tccatctcaa agtccgtacg gcgccagaga cgaagcagcg cttcgaactc
     2641 acgactcaga tgtactttga cgacgcgctc tcggatcgtg tctttgcaga tccgccgtat
     2701 gccgagaaag gatctcgcgc cggtcgaaac caagatgatc gcattttccg tcgcggagga
     2761 gaacagctcc tgctcagtct tatgcccgcg gccgagggct tcagtgcgac cttttccctg
     2821 ggcctgcagc ttccctcgtc ctgagccggc gcctgcgtag agttccaaca atggatcaga
     2881 agaggatcgt catcatcgga ggcggctttg gcggactcac agccgcccga tcactccgtc
     2941 gggccgacgt cgtgctcatc gaccgcacga atcatcacct gttccaaccg ctgctctatc
     3001 aggtggcgac cgctgccttg tcgccgagtg atattgcatg gccattgcgc accgtttttc
     3061 gctcgcaacg caacgtccgc gtcatgatgg atgaagtgct gtcgatcgat cgcgctgatc
     3121 gctcgatctg cttgcgtgag agcccgcccg tctcgttcga cgtcctcatc gttgcgcctg
     3181 gttcgcgtca tgcctatttt ggccacgacg aatgggaaga atttgcgcca gggctgaaga
     3241 ccatgaccga tgccgtacgg ctacgccaac acatgctgtc ggcctttgaa gacgtggaac
     3301 gccggctcgc ctctaccggc aaacatgaac cgctcacatt tgtgattgtc ggcggcggtc
     3361 ccaccggcgt ggaattggcc ggcgccctgg cagagattgg acgaaaggct atgggcccgg
     3421 attttcccaa cttgcggctc gatgatctct gcgttctact cgtcgagggc ggtccgcgca
     3481 ttttacccgg tttcagccca gatctttcag acaaagccgc ggcggccttg tctcgaatgg
     3541 gagtcaccat tcggctcaac agtctcgtga gcgctgtcga caaagggggt gtgaagattg
     3601 ggcaggactt cgtcccttcg gccggcatca tctgggcggc gggcaatcga gcttctggac
     3661 tcctcgatac gctgaaggcg gcccgcgatg gtgcggggcg ggtgatggtc aacccggatc
     3721 tgacggttcc tggggatccg tggatcttcg tcatcggcga tgcggctcat tgccgggggc
     3781 aggatggaaa ggcattgcca gggctggctc ccgtcgcgat gcagcagggc cgttacgtcg
     3841 cccagttgat cgaggagggt gcggctccca acactcgccc gccatttatc tacgctgacc
     3901 gagggatgct ggcgaccatt ggaagggcca aggcggtggc ccaaatcggc cggtttcatg
     3961 tttcaggact cctcgcctgg ctgctctggt gcattgtgca catctttttc ctcatcgggg
     4021 ttcgcagccg cctccgcgtg atgtcggaat ggatctggta ctacgtcacc ttcaagccgg
     4081 gcgcgagatt actcttcgaa caaccggagc cggcgcatcg ggagtcgcga ggatctcacc
     4141 gcaccggctg aacctcctgc ggattctctg gaccggttct cggcgaccat cagaatggcg
     4201 atggatcatc cgttgacacc gcgtgcagtg gctcgtagcc tatccacagc tgcccggtgg
     4261 aagaccgcat gaagccgcgt ataacgatcg tcacaatcgg cgtcgacgat cttgaccgct
     4321 cactacggtt ctaccgggac gggctgggtt ttcctactcg aggaatcgtc gggactgagt
     4381 atgaacacgg agccgtcgcc ttctttgacc tgacgaacgg ccagaaactg gcgatctggg
     4441 cccggaggga tctcgcccat gaggccaagg ttccgctggg acctcgcagc gcaagcgaat
     4501 tctctctcgg acacaatgtc gacagcaaag cggaagtcga tgcggtcatc agtcaagccc
     4561 agcgcgccgg agcaatgatt accgatccgc ctcacgaagc gttttggggt gggtacaccg
     4621 gatcgttcca agatcctgac gggcatctgt gggaagttgc gtggaatccg gaaatgaatc
     4681 cgcctgaata attggcagca agataggctg agacacaccg cagttcttat cggaggggtg
     4741 acctgatgcg ctcaagaatg gcatggacac tattgtggat gagtggattt gccttgtcag
     4801 cactagcggc aaacggggcc ggaaagaccg aagtgatgcc gatgatcaag gccgcgcccg
//
LOCUS       DASSCQ010000190         9005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_435700, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000190 DASSCQ010000000
VERSION     DASSCQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435700"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFH05_12330"
     CDS             complement(<1..1033)
                     /locus_tag="VFH05_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6679053.1"
                     /translation="MGLTDRDEGRNAEAGREMLETGNWVSPTFNYEPRFAKPALVYWL
                     MSLSYRWLGIDEFAARFPSAAFGGVLIVLQYLFVSRFCGAVVGLLSALMLLLNIEMIG
                     LSRMALTDGVLICFTTLSLYSFWLGFHGRQNERWWRWICYLAMGMATLAKGPVGFLVP
                     LVTIALYLTVTKQWRRFWHEGTPVAGLLLFALVALPWYLVMWSHHGAQYAASAQANTV
                     GRFLSPMEGHGFTILFYVPVLLLGFFPWSGWLPFAWYQAYRSWREDGGAIRISSGAQK
                     STPVVPASPSHSTTASLEWFAAAWVFGVFMFFTLSSTRLAHYIGPLFPAAALLTAMYW
                     HRSLHDVQTR"
     gene            complement(1138..1461)
                     /locus_tag="VFH05_12335"
     CDS             complement(1138..1461)
                     /locus_tag="VFH05_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HET6679054.1"
                     /translation="MLPDSMFSVEILWLAIGFLGQGLFFGRWLVQWIVSERKAESRMP
                     IAFWYLSLVGGLITLAYAIYRRDPVFIAGQGIGALVYIRNLMLIYRQGSHQHPGPVPS
                     VGKPQ"
     gene            complement(1465..2199)
                     /locus_tag="VFH05_12340"
     CDS             complement(1465..2199)
                     /locus_tag="VFH05_12340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6679055.1"
                     /translation="MTHSSQPWISVVVPIKDERDNLVSLTDQVMKVLHAREESHHAPF
                     EIIYVDDGSTDGSSQLLDRLAAEHASVIVLHFDRHYGQSSAFDAGFKRSTGALVVTMD
                     GDLQNDPADIGTLLAYTEKYDLVCGWRTERHDSLTRKISSRVANAVRSAVTGDHVHDT
                     GCSLKVFRREVVDRLQLFVGMHRFFPALALMHGFTVTEVPVRHHPRMHGISKYGVGNR
                     LFKGLYDLLAVRWMQDRVLKYRFRDK"
     gene            complement(2275..3978)
                     /locus_tag="VFH05_12345"
     CDS             complement(2275..3978)
                     /locus_tag="VFH05_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6679056.1"
                     /translation="MTDPVPHTSFPQYRLTNIVQDIVTQLGLLIRLERKMLGIIASYA
                     VAIGMFMLCVPIAVQELVSTFSFAIEPIMILTLTLVVGSALTGAAAFRILQARAVETL
                     QQRIYARIAIAFTETLPKIREEVFLPQHAHRFAEADLLTRALVAMVADLFYVVVVGSV
                     GMSLLVFFHPYFLLYNVILVVGFVFLLTVFGKGGFLITLEMSRLNYEMYHWMQNIAAN
                     LPHLRAVGNSPYLIRKTDELTRAYAEVRQRRSDLLTGRQYKAAALWQVVGHAGLIGTA
                     GMLVSQGQLTVGQFAAAELVAGNLLLNMDTLARRMVHMFFAFVSFRELNAFFSLPQDE
                     TASRTVVPLSQFGLAGIKVTARNLSFAYAGAPILFEHFHLDVAPGEKVVVLCHSNMQK
                     IALSRILAGLLAPSTGVVRYNDMNLVEVSLDSIDTCRSLMLDSHPTLLEGTIEDNITL
                     GRQSIGYEDVQWALEFVELDEEIDRMQEGLTTNVTGQSTQFTLSQILRLLLARAIVTR
                     PHVLIVEGTLHSMAPSVREVILRRLCSKDEPWSIIFVSNDPTFSRYVDRQIALEHLPS
                     V"
     gene            complement(4007..4384)
                     /locus_tag="VFH05_12350"
     CDS             complement(4007..4384)
                     /locus_tag="VFH05_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679057.1"
                     /translation="MAEGFEEELGFRRTHEIVPVKPTERFSPDVSGIFIVFTLHQHYQ
                     AFQVFGRCYPESVSGLDPQTMIGQDVMYIALEDESGYLKLSSPEGRWKPGRYKVEIHA
                     GEQVNEVSLMGTMRFTVAASHEP"
     gene            4673..6103
                     /locus_tag="VFH05_12355"
     CDS             4673..6103
                     /locus_tag="VFH05_12355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6679058.1"
                     /translation="MLQDADALPQLIGDWKAVDQWQAHINQLFYRFRGDQVRNFYQTF
                     ASADYRLAHALAADYFEQVSRRDKASRAQQAVAASQPLTVVELGPGNGNLAASFLSHL
                     RTLDREGRVYPRVRYVFVDWEQTVLDGAWNYPGLMDHRDKVQMLRSTIDHIEGLADGA
                     VDRIFCNELWNDLATKLMSRQGGDIEEEFLRPNLSDVLHAKIEDWAGFIRAFEAKDVD
                     QLKQFPPFFDDLVWEREYRTVEWKDVPYRKTITEFLRRIDDQVVVPVNIGAYLTMREA
                     KRLLAPDAVGFSSFDAGAADLKVLNDPDKPCSGQFGGQQSFMVNFALAEAVAKQVGGV
                     TASIEPQREFIGRSLGTNVLTLMDLLAMHPTAGHKLEPWEQDRLILKTIKALNVTYNS
                     PYAAKMDFPIRANVPSEAREALQALAEELKPAGVPDTVSYLTEEELTRASKDLEEVGY
                     DPQMFHIALTAPPAPVDYYHLSIRPS"
     gene            6275..6538
                     /gene="tatA"
                     /locus_tag="VFH05_12360"
     CDS             6275..6538
                     /gene="tatA"
                     /locus_tag="VFH05_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6679059.1"
                     /translation="MFGSFGWMELLLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSV
                     HEADAIDVTPPPVEPAPPQPTPMAQGQAAQPAPAQPEQQVKQG"
     gene            6622..6921
                     /locus_tag="VFH05_12365"
     CDS             6622..6921
                     /locus_tag="VFH05_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET6679060.1"
                     /translation="MFGLGAGEILIILVIAFLLFGPKQLPEVGRQVGKAVKGFKETAE
                     DLRKSVEPELNMIQQEVKMVEQDFQASMKEAEEEINQATTPADKPAGSSGTGNQA"
     gene            7245..8954
                     /locus_tag="VFH05_12370"
     CDS             7245..8954
                     /locus_tag="VFH05_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HET6679061.1"
                     /translation="MRRLLGRTGWVSISTFFSAALMAAGLSFTGAPAQAAFELPEGEK
                     ITNLPAIPRAIPQKEAYELYDPKIGKNFDIKNFWMRADLRVRPEMRNNVCFGGGAPVG
                     GACNGPAAIGAAPGARTANAGTKANDVYVQQWVRLGLGYDLSPDVNFYMEIIDAAVWG
                     GNGNQLNAGNGGDPLNHNGTSTGTGINGNGGRLGVRAAYMLVRNFAGIQGLSVKAGRQ
                     YVIFGNHSLFGHFDWANTGYSHDGIMLQYSTKAWDVYGGWFRNSEGDLPQASPVGSLA
                     GPVAGNAQNGQDGGRDADMFIFYNQIKSVPGFLIEPYYVYYSNRYASQDNSGQGLGTA
                     KHSNQSRHMVGNRIEMRKGNFDFINETSYQFGSMGSAGGVNAIQNGYGQQQNLHINAW
                     ATRNWIGYTHYQSSWKPRIAVNFDYASGDGRANCTISAAAGNCSSANTFENFFPTNHI
                     HMGYMDIQAWKNMMSPSGNLQFRPSKDDHFEIWYTHLNLANSRDNWYRGAQGVYVFSK
                     AGNTARHIGQEVDFTWTRMFMDGKMAFQATYGHLFAGSYIEQNLGQANDQQWAYVQLW
                     MNF"
BASE COUNT         2144 a         2457 c         2614 g         1790 t
ORIGIN      
        1 ctctcgtctg aacatcgtgg aggctccggt gccagtacat tgcggtgagc agcgcggcgg
       61 ctggaaacag tggtccgata tagtgagcga gcctggtgga cgacaacgta aaaaacatga
      121 agacgccgaa gacccaggcc gccgcgaacc attccaagct tgcggttgta ctgtgagacg
      181 gtgaagcggg tacaaccgga gtactctttt gcgcgccgct tgagatgcgt attgcgcctc
      241 cgtcctctcg ccaacttcga taggcttgat accaggcaaa tggcagccag ccgctccagg
      301 ggaaaaatcc caacaagagg acggggacat agaacaggat tgtgaaacca tggccttcca
      361 tcgggctgag gaagcggccg accgtattgg cctgtgcaga tgccgcgtac tgcgccccgt
      421 ggtgagacca catgacgaga taccagggga gtgccacaag ggcaaacagc aaaagtcccg
      481 ccaccggagt tccctcgtgc cagaaccgac gccattgttt ggtcaccgtc agatacagcg
      541 caatggtgac gagcggaaca aggaatccga ccggcccttt tgccagggtg gccattccca
      601 tcgcgagata acagatccac cgccaccagc gttcgttctg ccgtccatga aagcccagcc
      661 aaaaactgta caaggacagt gtcgtaaaac aaatcaacac gccgtcggtc agggccatac
      721 ggcttagacc gatcatctcg atgttgagca gtaacatcaa ggcgctgagc aggcccacca
      781 ctgccccgca gaatcggctg acaaagagat attgcagcac aataagcact ccgccgaagg
      841 cggctgaggg aaagcgggcg gcgaattcgt caattcccag ccatcggtag gacaggctca
      901 tcaaccaata cacgagagca ggtttggcaa aacgtggctc gtagttgaaa gtgggactca
      961 cccagttgcc cgtttccagc atctctcgcc ccgcttcggc attgcgccct tcatcccgat
     1021 cagtcaatcc catcgagcct aatccaagaa aaaacaggag acctgacaaa acgatgagga
     1081 tagccaggtt caccatgtgt ccggatatcg gcgaagcggg cgtgtgcatg agaggcgtta
     1141 ctgaggtttt ccgaccgaag gaaccggtcc tgggtgctgg tgtgatcctt ggcgataaat
     1201 cagcatgagg tttctgatgt agaccagtgc tccaattccc tgtccggcga taaagaccgg
     1261 atcacggcgg taaatcgcgt aggccaatgt aatcaaccct ccgaccaggc tcagatacca
     1321 gaaggcgatg ggcatccgac tttcggcttt tcgctccgag acgatccatt gtacgagcca
     1381 tcggccgaaa aacaggcctt gcccgaggaa accaatggca agccagagga tttcaactga
     1441 gaacatggaa tccggcagca tcgatcactt gtcgcggaag cggtatttta agacacgatc
     1501 ctgcatccag cgaacggcca gcaggtcgta gagtcctttg aagaggcggt taccgacgcc
     1561 gtatttcgat atgccatgca tccgcggatg atgacggacg gggacttcag tcacggtgaa
     1621 tccgtgcatg agggcaaggg cgggaaagaa ccggtgcatg ccgacaaaga gttgcagccg
     1681 gtcgacgacc tcccggcgaa agaccttgag cgagcatccg gtgtcgtgaa catggtcgcc
     1741 tgtgacagcg ctccgcacgg cgtttgccac ccgggacgaa attttccgtg tgagactatc
     1801 atgccgttca gtccgccacc cgcagaccaa atcgtacttt tccgtgtagg ccagcagcgt
     1861 gccgatgtcg gccggatcgt tctgaaggtc accgtccatc gtcacgacca gcgcgccggt
     1921 cgaccgcttg aatcctgcat cgaatgccga agattgtcca tagtggcgat cgaaatgcag
     1981 cacgatgaca gacgcatgct cagcggcgag gcggtccaat aactggctgc tgccgtcggt
     2041 gctgccgtcg tccacataaa tgatctcgaa cggggcatga tgcgattcct ctcgagcgtg
     2101 caacaccttc atgacctggt cggtcaatga gacaagattg tcgcgttcgt ctttaatagg
     2161 aacgacgact gagatccatg gctgggatga gtgggtcatg aggagtggat acgcgcagca
     2221 cgtcaatcat gcggagaaat caccacggca ttgtacagaa ggccttgggg agattcaaac
     2281 tgaaggcagg tgttcgagcg cgatctggcg gtcgacatac ctgctgaacg tggggtcatt
     2341 ggagacgaaa atgatggacc atggttcatc cttggaacat agacggcgca aaatcacttc
     2401 tcgcacagag ggggccatgc tgtgcagagt gccttcgacg atcaggacgt gcggtctagt
     2461 aacgatggcg cgcgcgagga gcagcctgag gatctggctc aaggtgaact gggtgctctg
     2521 gccggtgaca ttggtcgtga gcccttcctg catccgatcg atttcttcat cgagctccac
     2581 gaattcaagg gcccattgga cgtcttcgta cccaatcgat tggcgcccga gcgtgatgtt
     2641 gtcctcgatc gtgccttcga gcaacgtagg atgcgaatcc agcatcagac tgcggcatgt
     2701 gtcgatcgag tccagactga cctcgacgag gttcatgtcg ttgtaccgca ccacgccggt
     2761 cgatggcgcg aggagtccgg ccagaattct ggaaagcgcg attttttgca tgttggagtg
     2821 gcagagcact accactttct cgcccggggc gacgtcgagg tgaaagtgct caaagaggat
     2881 cggagcgcca gcataggcaa aagataagtt tctggctgtc actttgatgc ctgccaggcc
     2941 gaattgactc aggggtacga cggttcggga ggccgtctcg tcttgaggaa gtgaaaaaaa
     3001 tgcgttcagt tctcgaaatg agacgaacgc aaaaaacatg tgaaccatcc ggcgggccag
     3061 ggtgtccatg ttgagaagga ggttgccggc aaccagctcg gctgccgcaa actggccgac
     3121 ggtgagctga ccttgtgaca ccagcatgcc ggcggttccg atcaacccgg catgtccaac
     3181 cacctgccag agggcggccg ccttatattg ccgtccggtc aagagatccg accgccgttg
     3241 gcgaacttcg gcataggccc tcgtcagttc gtccgtcttc cgaatcagat acgggctgtt
     3301 gccgaccgcc cgcaaatgcg gcaggttggc agcgatattc tgcatccaat gatacatctc
     3361 gtaattgagc cgggacatct ccagggtaat gaggaacccg cctttcccaa acacggtcag
     3421 aagaaagacg aatccgacga cgaggatgac gttgtagagc aaaaaatacg gatggaaaaa
     3481 gacgaggagc gacatgccaa cggagcccac gaccacaaca tagaagagat cggccaccat
     3541 cgcgaccagc gcgcgtgtca acaggtccgc ctcggcgaac cgatgggcat gctgcggaag
     3601 gaatacttct tcccgaatct tgggaagcgt ttccgtgaat gcgattgcga tgcgcgcgta
     3661 aatccgctgt tgcaacgtct cgacggcccg ggcttgcaag atacgaaagg cggcggcacc
     3721 ggtcagcgcc gacccgacca cgagggtgag cgtcaagatc ataatgggct cgatggcaaa
     3781 ggaaaatgta ctgaccagtt cttgtacggc aatagggacg cacagcatga acatgccgat
     3841 ggcgacggca tacgacgcaa tgatgccgag catcttccgt tcgagacgga tgagaagtcc
     3901 gagttgagta acgatatctt ggacgatgtt cgtgagccga tattgtggaa aggaggtgtg
     3961 cggaacggga tcggtcatgg tcggccagtt ctggccttcg gcgttgtcat ggttcgtgag
     4021 aggccgccac ggtgaagcgc atggtaccca tgaggctgac ctcattaacc tgttcccctg
     4081 cgtgaatctc taccttgtac cggcctggct tccaacgccc ctccggggac gacagcttca
     4141 aatagcctga ctcatcttca agggcgatgt acatcacatc ctggccgatc atcgtctgcg
     4201 gatcgagtcc ggaaaccgat tcgggatagc agcgaccgaa gacttggaag gcctgatagt
     4261 gctgatgcag cgtgaacacg ataaaaatgc ccgacacatc ggggctgaac cgctcggtcg
     4321 gcttgaccgg gacaatttcg tgggtgcggc gaaagcctaa ctcttcctcg aacccttcag
     4381 ccatcgtgat actccggaac agcccttgag gaggcgcgtc gaaatcgtaa ccgccttgtt
     4441 ctagtggcgc ggattctcct tcagggctcg ggcgtgtcat aagtgcttcg ccagccggga
     4501 ggatcggggg ggaggccagc caccagagaa gcaggggaac ccagatagcc agacggatac
     4561 ccatgaatcg ttcgtacact gccagactgg gactgtcaag gagcagcgcc agttgcggcc
     4621 gttgaaatcc atctgttaga atttcggccg cgacaaggaa tctccacgga ctatgctgca
     4681 ggacgcggac gctttacccc agctcatcgg cgattggaag gccgtcgacc aatggcaggc
     4741 gcacatcaac caactcttct atcgcttccg gggcgatcaa gttcgtaatt tttatcagac
     4801 gttcgcctcc gccgattacc gcctggccca tgcgttggcg gcagactact ttgaacaggt
     4861 atcccgccgc gacaaggcct cacgggccca gcaggctgta gccgcttccc aacctttgac
     4921 ggttgtcgaa ctcggcccgg gcaatggcaa tttggctgcc tcctttctca gccatcttcg
     4981 cacactcgac cgcgaggggc gggtgtaccc gcgagtccgc tacgtctttg tggattggga
     5041 gcagacggtg ttggatggag catggaacta tccagggctg atggaccatc gtgacaaggt
     5101 ccagatgctc cgatcgacga tcgatcacat cgagggactc gccgatgggg ccgtggatcg
     5161 catcttctgt aatgaacttt ggaacgactt ggccaccaag ctgatgtcac gacaaggcgg
     5221 cgacattgaa gaggaatttc ttcgccccaa tttgagtgat gtcttgcatg cgaaaatcga
     5281 ggactgggcg ggatttattc gtgccttcga agccaaggat gttgaccaac tgaagcaatt
     5341 tcctcccttt ttcgacgatc ttgtgtggga gcgcgaatac cgtacggtag aatggaaaga
     5401 tgtcccgtat aggaaaacga tcacggagtt tctgcgtcgc atcgacgatc aggtggtggt
     5461 gccagtcaat atcggcgcct atctcaccat gagagaagcg aaacgccttc tcgcgccgga
     5521 tgcggttgga ttcagcagtt ttgacgccgg agccgccgat ctcaaggtcc tcaacgatcc
     5581 ggacaaaccc tgctcggggc aattcggagg acagcaaagc ttcatggtga atttcgcact
     5641 cgctgaggca gtcgccaagc aagtcggcgg cgtcacggct tcgatcgaac ctcagcggga
     5701 atttatcggc cggagcttgg gaacgaatgt gttgacgctc atggatctct tagccatgca
     5761 tccgacggca ggccataagc tcgaaccatg ggagcaggat cgcctgattc tcaagaccat
     5821 caaggcgctt aatgtgacgt ataacagccc gtatgcggcg aagatggatt tcccgatccg
     5881 cgcgaatgta ccgtctgagg cgcgagaggc cctgcaggca ttggccgaag agctcaagcc
     5941 cgctggagtc cccgatacgg tttcctacct caccgaggag gaattgactc gtgcgtcaaa
     6001 agaccttgaa gaggtggggt atgatccaca gatgtttcac atcgcgctga ccgcgcctcc
     6061 cgctccggtc gattactacc acttgtccat ccgcccttcc tgatattggg ttccagaccc
     6121 ctacccatgg cccccttccc tgaaattaga cttgactaat ctcacgtatt tctatagcct
     6181 cccacccaat ttgacaaaga tccccacctt ttgggttacg taatcttgcg tttgccgtgt
     6241 ccgggggaga catggaagaa agagaaattt cctgatgttc ggttcgttcg gctggatgga
     6301 attgctcctc atcctgatca tcgtgctcat cattttcggg gccggcaaaa ttcctcaatt
     6361 aggcgaggga ttaggaaagg ccattaaggg atttaaaaag agtgtccatg aagccgatgc
     6421 cattgatgtg acgccgccgc cggtcgagcc ggcgccgccc cagccgacac ctatggcaca
     6481 aggacaggct gcccaaccgg ctccggctca accagagcag caagtcaagc aaggataact
     6541 gacgtgaggc gttgtgcgtc agccgtgaag cggatgagga aaatccacgc attacgtttc
     6601 acgcatgacg tatgacgacc tatgttcgga ttaggtgcag gcgaaatact cattatcctg
     6661 gtcatcgcgt ttttactgtt tggccccaag cagttgcccg aagtcgggcg gcaagtgggc
     6721 aaagcggtaa aaggctttaa ggaaacggcc gaagacctgc ggaaatcagt cgagcccgaa
     6781 ctcaacatga ttcagcagga ggtcaagatg gtcgaacagg atttccaggc ttcgatgaaa
     6841 gaagcggagg aggaaatcaa ccaggccact acgccagctg acaagcccgc tggatcttcg
     6901 ggaacgggca atcaagcgta ggacctggag tagccgggct cttcgataag gcagggaagg
     6961 gggtgaccgc ctctcgaccc gatcggcggt ggcagggcag cgaggacgaa gggtcgtatt
     7021 caaaaagagc aaaattccta ggcaaacaag gagtattgat agacactacc aggtgtcatg
     7081 agtccgtggt tcgaggaaag cgtgggcaca cggctttcca aaccggcaaa cgaaggaaaa
     7141 gaagtttgtc agtgcgagcg cagttggatg tcaagtcggc agccaggacc ggtagagagt
     7201 cccggagcgg tgaaggctgt aatccaattc acaggaggaa tgtcatgaga agattgctag
     7261 gtcggacagg gtgggtcagc atttccacct tcttcagcgc cgcccttatg gcggcgggcc
     7321 tgtcgttcac aggagcacca gcccaagcgg ccttcgagtt gccggaggga gaaaagatca
     7381 cgaacctccc ggccatcccc cgtgcgatac ctcaaaaaga agcctacgaa ctctacgacc
     7441 cgaagatcgg gaagaacttc gatatcaaga acttctggat gcgcgcggac ctccgcgtgc
     7501 gccccgaaat gcggaacaac gtctgcttcg gcggcggtgc gccggtgggc ggagcgtgta
     7561 acgggccggc tgccattggt gcagcgccgg gagcccgaac cgcgaatgca ggcacgaaag
     7621 ccaatgatgt gtatgtccag cagtgggttc ggttgggctt gggctatgac ctctcgcccg
     7681 acgtgaattt ttacatggaa atcatcgatg ccgccgtatg gggcggcaat ggcaaccagc
     7741 tcaatgcggg caacggcggt gatccactca accacaatgg tacatccaca ggaaccggta
     7801 tcaatggaaa cggtggacgt cttggcgtcc gtgctgccta catgttggtg cgtaatttcg
     7861 ccggcatcca gggcctcagc gtaaaagcgg gccgccaata tgtgatcttc ggcaaccact
     7921 cgctgttcgg ccacttcgat tgggccaaca ctggctacag ccatgacggt atcatgctgc
     7981 agtacagcac caaggcctgg gatgtgtacg gagggtggtt ccgcaattct gaaggtgatt
     8041 taccgcaggc ctctccagtc ggaagcctcg ccggtccggt cgccggtaac gcacaaaacg
     8101 gacaagacgg tggccgagat gccgacatgt tcattttcta taaccagatc aaatctgtgc
     8161 ctggtttctt gatcgaaccc tattatgtct actactccaa tcgatatgca tcacaagaca
     8221 actcaggaca aggtcttgga accgcgaagc actcgaatca aagtcgtcac atggtcggta
     8281 accgcataga gatgcgaaaa gggaacttcg acttcatcaa cgagacctcc taccagttcg
     8341 gatccatggg tagcgcgggt ggcgtcaatg ccattcagaa cggctatggt cagcagcaga
     8401 acctgcacat taatgcctgg gcgacaagaa actggatcgg ctacacgcat taccagtcat
     8461 cgtggaagcc cagaatcgcc gtcaacttcg attatgcctc gggtgatggc cgcgcgaact
     8521 gtacgatcag tgcggccgct ggaaactgta gctcggcgaa tacattcgaa aatttcttcc
     8581 ccacaaacca catccacatg ggctatatgg acatccaggc ctggaagaac atgatgagcc
     8641 cttcgggcaa ccttcaattc cgtccctcca aggatgatca cttcgagatc tggtacacgc
     8701 acctgaactt ggcgaactcc agggacaact ggtatcgtgg cgcccagggt gtctacgtct
     8761 tctcaaaggc cggcaatact gcgcggcaca tcggtcagga agtcgacttc acctggaccc
     8821 gtatgttcat ggatggcaaa atggccttcc aggccaccta tggacacctg ttcgccggct
     8881 cgtacatcga gcagaacttg ggccaggcta acgaccagca gtgggcctat gtccagctct
     8941 ggatgaactt ctaagacgaa gctcgtcaaa gctgggtgtg aggcgcgatc cgcctgatgc
     9001 ctgaa
//
LOCUS       DASSCQ010000191         5596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_931040, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000191 DASSCQ010000000
VERSION     DASSCQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931040"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..655
                     /locus_tag="VFH05_12375"
     CDS             473..655
                     /locus_tag="VFH05_12375"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET6679062.1"
                     /translation="MGKIVECAKVDPSSGCTHVIRGTTEEEVLKNAAEHAKQHGIREV
                     TPELKAKVKAAIRDEK"
     gene            1106..2770
                     /locus_tag="VFH05_12380"
     CDS             1106..2770
                     /locus_tag="VFH05_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6679063.1"
                     /translation="METLKTHFKRMSALGFAATVLLFCAAPSWGTESAVAPGPKDLSH
                     TVPLYTNLGSHHKPISTQVAATQEYFDQGLRFMYGFNHAEAIRSFTRAAELDPTCAMC
                     YWGIALAYGPNVNAPMDSASGVAAYAAAQKAMTLRSHATEPERAYIEAVAQRYEAVPP
                     TDRARLDTAFSRAMKRVVNTYPDDLDAATLYAESLMDLRPWNYWQPDGTPYPGTNEIV
                     RQLQRVISRNPNHPGACHYYIHAVEAVNPEAAVPCAERLARLMPGEGHMVHMPAHIYI
                     RVGRWNDAVQANQHAIHNDEVFIEGQHPTGVYPLAYYPHNIHFLAFASTMAGRSMQAI
                     EASTTLTSKVDLDAARQVGMLQEMLPYRALTLTTFGKWDEVLAEPLPPADLRFSYAMA
                     HYARGVAQAAKARWAEAQAALDTVREINAATPEGADGKTALSIAVSALSGEIATRRGD
                     LDTGINYFREAAKIEDAGLYFEPPKWYYPIRHSLGAALLKKGQHAEAEKVYREDLRRF
                     PENGWSLFGLAQALRAQSKNTEAAEVESRFSRIWSSTDVTLTASRF"
     gene            2806..4026
                     /locus_tag="VFH05_12385"
     CDS             2806..4026
                     /locus_tag="VFH05_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HET6679064.1"
                     /translation="MTPPESFDDHSQTLSALSGVSRRDFLIRGSTLLAALALSDSPLY
                     AEMWRISEGEKVVPFLDQAPAPPDAAIRQYGDLNTRNWEQLSSWTTPNEDFFTVSHYN
                     RPVLKAEEWRLNIDGLVERPISLNLEQIHARPRQEVTFTLECGGNHGFDWFTGGIGNA
                     RWAGTPLAPILRAAGIKKAGIDVVFFGSDEGDEEVRDIKMKQNFSRSMSIEEALAPAN
                     LLCYEMNGEPLPPLHGFPLRLIAPGWYGVANVKWLKRIEVVDKRWAGRFMAKDYVTIR
                     QEPRDNGELVWTQKVVGPALLKSVTAKVTVKNGTHRIYGAAWGRPIQRVEVRIDDGPW
                     RAATIDRGQEHEFAWKFWHLDWKEAVAGEHTITSRAIDRAGEVQPAMGEPVIANKRTY
                     WESNGQITRRIRIG"
     gene            4192..4518
                     /locus_tag="VFH05_12390"
     CDS             4192..4518
                     /locus_tag="VFH05_12390"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6679065.1"
                     /translation="MDLRRHQRFPVRFHTVFSGPKLSESVGTVINLSQSGCCLETDCQ
                     VYTGIQLALRLEVPDETPIHIERAAVRWNRGRQLGVGFITIASPHQERMAQLLAKLKP
                     GSEAAP"
     gene            4821..5348
                     /locus_tag="VFH05_12395"
     CDS             4821..5348
                     /locus_tag="VFH05_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679066.1"
                     /translation="MRSMQRVAGFLIGAVVGAVLLGTTSLAVAAEKGKGHEAHKHFKG
                     EVIEKAGALAIKTEEGTTYQLSENEARRQGREFKAGDKVEVSVNENNAIVDVHLQGEK
                     AEHKFVTGKLIHVGRMKKEIKLQTAEGEQSFPLLLQETKTKDLPEGTEVTVELNEAGT
                     VIDLHKGKHVGQKKQ"
BASE COUNT         1292 a         1635 c         1568 g         1101 t
ORIGIN      
        1 catgtcaacg ttctcgtaac tatacgcggg tcgttggcga cctggattag ggtcatcggc
       61 acaaccagcg accatgaggc cggccataag gacaacgggc agggtccata aaatacgact
      121 catgctgaac ctccttggtc accctcagtt attccatctt tcactttagg aaggttgttc
      181 ttcccttcct tttcatacaa gcagtctatg tccttaccag acaatgcata cctagcaagc
      241 ccccttgaaa cacgaaaagg ggggacattc actcacgaaa tatacccagt ttaataccat
      301 ttcggcgtcg cgggcacccc ataattgcat ccatagctag tgatgactgc agacggccat
      361 tgactctctc ctccaatgtc ctcaaaattt ccctatcgtt cgcgcattca ttaacaaggt
      421 tttgacagcg gtcggatacg agcaggtcct ggctgaacaa ggaggtgcga tcatggggaa
      481 aatcgttgag tgcgccaagg ttgatccatc gtccggatgc acacatgtca taaggggcac
      541 caccgaagag gaagtgctga agaatgcggc ggaacacgcg aagcagcacg ggattcggga
      601 ggtcacacct gagctcaagg caaaagtcaa agccgcaatc cgagacgaga agtaacttat
      661 cctagactat ccctctggct cgctagcgcg cattttaata ttgagagcca gtggcgtgat
      721 ccgtcccgcc tcgctaatgg acgagggaga accctcccgg gtttcttccc tcgttctgtt
      781 ctgctaaccc ggtaacaaaa acgcactgtg acaccgtgac gtatgtcgag actcgccatg
      841 cgaatcgact cctctcttca ctgttttgag tattcgccaa catctcccct gtgaattcca
      901 ccctcgttgc ggcgcttcgg caaataaatc cggacgccaa agcaagtaca cgcaggccag
      961 cacgcctctg ttaagagaat cagactcccg gataagactt caacgcccat agcccctata
     1021 gtgcgaggcg tcgttgcgca ctgatgcgag aaccgtcacg cacacacagg aggacaatat
     1081 gaggaccaac gtacgatcat ccgacatgga aactttgaag actcatttca agcgaatgtc
     1141 agcgctcgga ttcgccgcga cggttttgct cttctgtgcc gcccccagtt ggggcacaga
     1201 atcggcggtg gcacccgggc cgaaggacct ttctcacaca gtccccctct acacaaacct
     1261 cggctcccat cacaagccca tctccaccca agtcgcggcg acacaggagt acttcgacca
     1321 ggggttgcga ttcatgtacg gattcaatca cgcggaagcc atccggtcgt tcacgcgggc
     1381 cgcagaactc gatcccacct gcgccatgtg ctactggggc atcgcattgg cctacggccc
     1441 gaacgtcaac gctccaatgg actcggcgag cggagttgcc gcgtacgccg cggcgcaaaa
     1501 ggcgatgacg ctcagatccc acgccacgga acctgagcga gcctacatcg aggcggtggc
     1561 gcagcggtac gaagcggttc cgccgaccga tcgtgcccgg ctggacaccg cgttctcgcg
     1621 cgcgatgaag cgggtcgtca acacctaccc tgacgatctc gacgcggcga ccttgtacgc
     1681 tgaatcgctg atggatctcc gcccgtggaa ctactggcaa ccggacggta ctccctaccc
     1741 cggcaccaac gaaatcgtcc gtcaactcca gagagtcatc tcgcgcaacc cgaaccaccc
     1801 cggcgcgtgc cattactaca ttcacgccgt cgaagcggtg aacccggagg ccgcggtgcc
     1861 ctgtgcggag cggctcgctc ggctcatgcc gggcgaagga cacatggtcc acatgcccgc
     1921 acacatctat atccgcgttg gacggtggaa cgatgccgtt caggccaatc aacacgccat
     1981 tcacaacgac gaagtgttca ttgaagggca acacccgaca ggtgtctatc cgctcgccta
     2041 ctacccgcac aacatccatt tcctcgcctt cgcctccacg atggccggac gcagtatgca
     2101 ggcgattgag gcctccacga cactcacctc caaagtggac ctcgacgcgg cacgtcaagt
     2161 cgggatgctg caggagatgc tgccgtaccg cgcgctgacg ctgacgacgt ttggaaagtg
     2221 ggatgaagtg cttgccgaac cgcttccgcc ggctgacctt cgcttctcgt acgccatggc
     2281 acattacgct cggggcgtcg cgcaggccgc caaagcacgg tgggcagagg cgcaagccgc
     2341 gctcgacacc gtgagggaga tcaacgcggc gacgcccgaa ggtgccgatg gcaagacggc
     2401 gctctcgatc gcggtgagtg cgctgtccgg cgagatcgct acccgacgag gcgacctcga
     2461 caccggcatc aattacttcc gggaggcggc caagattgag gacgccggac tttacttcga
     2521 accgcccaag tggtactacc cgattcgcca ttcgcttggc gccgccctgt tgaagaaggg
     2581 gcaacacgcc gaagctgaga aagtgtaccg cgaggacctt cggcgcttcc ccgagaacgg
     2641 ctggtccttg ttcgggctcg cacaggcgct tcgggcacag agcaagaaca ccgaagcggc
     2701 cgaagtggaa tcgcgcttca gccgtatctg gtcaagcacg gatgtgacgt tgacggcatc
     2761 gaggttctag cacgccaatg cctacgctca gaaaggaaga ctacgatgac accgccggaa
     2821 tcttttgacg atcactcaca gacactttcc gccctctccg gcgtttcacg acgcgatttt
     2881 ctgattcgtg gaagcaccct gctcgcggcg ttggccttgt cggattctcc gttgtatgcc
     2941 gaaatgtggc gtatcagcga gggcgagaag gtcgtccctt ttttggatca agctcccgcc
     3001 ccaccggatg cggccattcg acaatatggc gacttaaaca cgcgcaattg ggagcaattg
     3061 agttcctgga ctactcctaa cgaggatttt ttcaccgtca gtcactacaa ccggccggtt
     3121 ctcaaggctg aagaatggcg gctgaacatc gatgggttgg tcgaaaggcc gatctcgctc
     3181 aacttggaac agatccacgc gcggccgcgg caggaggtga cgtttacgct ggagtgcggc
     3241 ggcaatcacg gcttcgactg gttcaccggc ggcatcggga atgcgagatg ggcaggaacg
     3301 ccgctcgctc caattttacg cgcggccggc atcaagaagg ccgggattga tgtcgtcttc
     3361 ttcggcagtg acgaaggcga cgaagaggtt cgtgacatca agatgaagca aaacttcagc
     3421 cgaagcatgt cgatcgagga ggcgttggct cccgcaaatc tcctctgcta tgagatgaac
     3481 ggcgagccgc tgcctcccct gcatgggttt ccgctgcgtc tcattgcgcc gggatggtat
     3541 ggggtcgcca atgtcaaatg gctcaagcga atcgaggtcg tcgacaagcg ctgggccggc
     3601 cggttcatgg ccaaggacta tgtcacgatt cgtcaagagc cgcgcgacaa cggggaactc
     3661 gtgtggacgc agaaggtggt tgggccggcg ttgctcaaat ctgtgacggc gaaggtgaca
     3721 gtgaagaacg gaacgcatcg aatttatggt gccgcctggg gaaggcccat tcaacgtgtg
     3781 gaagttcgca tcgatgacgg accatggcgt gcggccacca ttgatcgtgg gcaggagcat
     3841 gagttcgcgt ggaagttctg gcacttggat tggaaggagg ccgtagcggg cgagcatacc
     3901 atcacatcgc gggccatcga cagagccggg gaggttcaac cagccatggg ggaaccggtc
     3961 atagcgaaca agcgtaccta ctgggagagc aatgggcaga tcacgcgccg cattcgaatt
     4021 ggttgagacg gcggccgcat caccttgcca atgtaagagt caggcagctc catgggcgat
     4081 tgggcatgcg cttatcggcc ttctgagtcc ttgacacatc ttcaccgagg gcctagcctt
     4141 aaacagttta cccagcagtt caaccgcaca ctcagcggaa ataggttcct catggacctt
     4201 cgtcgtcacc aacggtttcc ggtgcgtttt cacaccgtgt tctccggacc gaaattgagt
     4261 gaatctgttg gaacggtcat caatctgtca cagagcggtt gctgccttga aaccgactgt
     4321 caggtctaca caggcatcca attggcctta cgccttgaag ttcccgacga gacgccaatc
     4381 catattgaac gagcggcagt gcgttggaac cgcggacgtc agttaggcgt gggattcatc
     4441 acaatcgctt caccgcatca ggaacgaatg gcccaacttc ttgccaaatt gaaaccaggc
     4501 tcagaagcgg ctccataggt ctactcaatt cgcccatttt tttgtcttgc ctacccttca
     4561 cagcggggac atgctgaatt gtgtggcacg tggcgggcgg ctaaatcagc cgatctttgt
     4621 tgaacgtctg ccatccatgc actgcggacc cctaccgcca atttcaacgt tcagagttta
     4681 acgaggtcgg tgataaaatt tcggctgccg tcccaagctc aagtgaatct cgcattgatt
     4741 ccgatatctc aagacactat gatacttcgc aaggccgctc atcgatgagg gccatccatt
     4801 cgcaaactag gaggatgcac atgcggagca tgcagagagt cgctgggttc ctgatcggtg
     4861 ccgtagtcgg agccgtcttg ctgggaacaa ccagcttagc tgtagcggct gaaaaaggaa
     4921 aaggacacga agcgcacaaa cacttcaagg gcgaagtcat cgaaaaggcc ggcgccctgg
     4981 ccatcaagac agaggaagga acaacgtacc aactcagtga aaatgaggca cgtcgccaag
     5041 gtcgcgagtt caaagccggc gacaaggtcg aggtgtcggt caacgagaac aatgccatcg
     5101 tggacgtgca cctgcaagga gaaaaggccg agcacaagtt cgtgacggga aagctcatcc
     5161 acgttggcag aatgaagaaa gagatcaagc tgcaaacggc ggaaggggag caatcgtttc
     5221 cgctcctcct gcaggagacg aaaaccaagg accttcccga gggaacggaa gtcactgttg
     5281 agttgaacga ggcagggacg gtcatcgatt tgcacaaggg caagcacgtt gggcagaaga
     5341 aacagtagtg atccacaccg aggtgcgtac ctgcttttga gctcagaacg cacacccgtc
     5401 acgtgtaaga cgtgacgggg tgctgtctga atttcgcggc acctggcagg cccttccctc
     5461 gttcgccttc cggccccaat cgccgttcat cgcacgtcat acctaggtag accctgctac
     5521 cttaaaacgt agaccgtccc ggctccttcg attctcggtt atatccccat tgaacgtgcc
     5581 cccaatcctg cgattt
//
LOCUS       DASSCQ010000192         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_339361, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000192 DASSCQ010000000
VERSION     DASSCQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339361"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFH05_12400"
     CDS             <1..757
                     /locus_tag="VFH05_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6679067.1"
                     /translation="RELVPVPSGLDAAEAVSLVLNYVTAYQMLHGSANVRPGQRVLIH
                     GAAGGVGSALLQLGRLARLQMYGTCASHGASTVSSLGGIPIDYQHQDFVTEIRRFTNE
                     GVDVVFDGIGGTHIWRSRKALRSGGTVVAYGITSSLHGGRLTSGRSRGRHRFRAVAIF
                     GLYIAAGWLLPGRKRVVPYSIQWLKRLRPGLFRENLIALFDLLHEQKIKPLIAKRFPL
                     VEARQAHELLEKGGVTGKIVLVSDGFASSEPGV"
     gene            775..1725
                     /locus_tag="VFH05_12405"
     CDS             775..1725
                     /locus_tag="VFH05_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6679068.1"
                     /translation="MRTALWFFFTAVIAALGTATYIYQTDIQEARTRVATGSSLAHTP
                     CGPIEYAVRGDGPPVLVVHGAGGGFDQGLDFGAPLAKLGLRIIAMSRFGYLRTPLPPD
                     ASPAAQADAHACLLDALGIEKVAIVGGSAGAPSSMQFALRHPARLTALVLVVPATFVP
                     RPGNAPALHTPPETQLLFETALKWDVVFWAVSRLAPDAVIRSILATPPNLVRDAAPEE
                     QARVQQALKHILPISQRRQGLLNEAAVIPSLQRYELEKIHVPTLTISCADDAFGTYDG
                     ARYTAEHIPGARFIGYEAGGHLWVGHQQEMTDEIASFLKS"
     gene            complement(1785..2048)
                     /locus_tag="VFH05_12410"
     CDS             complement(1785..2048)
                     /locus_tag="VFH05_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679069.1"
                     /translation="MMPEREAWLYAAAVLGALASIAVGRTSGTAEELPVQARLWKFVK
                     SWLLVVALAALSILHGKWQPMVFFALVGGMSSALFRWGCKTSK"
     gene            complement(2131..2727)
                     /locus_tag="VFH05_12415"
     CDS             complement(2131..2727)
                     /locus_tag="VFH05_12415"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6679070.1"
                     /translation="MTRKTVTWIAVMTTMAALGAPGIFAVEKQSGPVGVPPETVADYL
                     RAVIMAHRHFYTIHIANRLRQERIVDVSENWRTTHSLPLPVQLVRETSEIAELTGPNV
                     RYRLISQWPINKANAAGTEFERRGLKEVQARPNRRYSSASTVPASAEQVFEAVYADIA
                     VTSACVECHNTHPSSPKKDFKVGDVMGGLVISFPLSKQ"
     gene            complement(2724..>2981)
                     /locus_tag="VFH05_12420"
     CDS             complement(2724..>2981)
                     /locus_tag="VFH05_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679071.1"
                     /translation="LVYRVDSINSGYVRISDEDPFGVLVPVQFGQAVASTCRPALRST
                     AEQFSSFLMTPRIQKLLVKYGFDSVPLPVSHVPIERKERAS"
BASE COUNT          614 a          873 c          869 g          625 t
ORIGIN      
        1 acgcgaactg gttcctgtgc cttcgggatt agacgctgcc gaggctgtca gcctggtgct
       61 gaattatgtg actgcgtatc aaatgctgca tggctccgct aacgtcagac caggccagcg
      121 cgtgttgatt cacggcgcgg caggcggagt cggctcggca ctgttgcagc ttggtcgtct
      181 cgcgcggctg cagatgtacg ggacgtgtgc ttcgcatggg gcatcgaccg tttccagcct
      241 cggcggtata ccaatagatt accaacatca ggacttcgtg acagagattc gccggttcac
      301 gaacgagggc gtggacgtcg tcttcgacgg catcggtggc acgcacatct ggcgttcccg
      361 caaggcccta cgttccggcg ggacagtcgt ggcctatggc attacctcgt cgcttcatgg
      421 agggcgattg acttcaggcc gttcacgagg tcgccatcgc tttcgtgcgg tcgctatctt
      481 cggattgtac atcgccgctg gctggcttct gccgggcaga aaacgcgtgg tcccctacag
      541 catacagtgg ctcaaacggc tccggcctgg gctgtttcgg gagaatctga tcgccttgtt
      601 tgacctcctt cacgaacaga agatcaaacc acttatcgca aagcggtttc cgcttgttga
      661 ggccagacag gcgcacgagt tgctcgagaa gggaggcgtg accggcaaaa ttgtgctcgt
      721 gtccgatgga ttcgcgagct ctgagccggg cgtgtgaaca tgacgagccg acctgtgcgc
      781 acagcgttat ggtttttttt cactgcggtc attgccgcac tgggaacggc gacgtacatc
      841 taccagacag atattcaaga agcgcgcacc cgcgtcgcga ctggcagcag ccttgctcat
      901 accccctgtg ggccgattga atacgccgtg agaggagacg gtccacctgt gttggtcgta
      961 catggcgccg gtgggggatt cgaccaagga ttggacttcg gtgcgccgct ggccaagctg
     1021 gggcttcgca tcatcgcgat gtcgcgcttc gggtacctgc gcacaccgct accgccagat
     1081 gcttcgccgg cagcgcaggc cgatgcacat gcttgcctct tggatgcgct cggtatcgag
     1141 aaagtcgcga tagtcggcgg ctctgccggc gccccatctt cgatgcagtt cgctttgcgg
     1201 catcctgcca gattgaccgc attagtcctc gtggttcctg cgacttttgt cccccgacca
     1261 ggcaacgcac cagccctgca cacaccgcct gaaacccagt tactattcga gacggcgttg
     1321 aagtgggatg tggtgttttg ggctgtgagc cggctcgccc ccgacgccgt aatccgttca
     1381 atcctggcca caccgccaaa tctggtgagg gacgcggccc cggaagagca ggcgcgagta
     1441 cagcaggcgc tgaaacacat attaccgatt tcacaacgtc ggcaggggtt gctcaacgag
     1501 gcggccgtga taccttcact ccagcgctat gagctcgaga aaatccacgt tccaacgttg
     1561 accatcagtt gtgctgacga tgcattcgga acctatgacg gcgcgcgcta taccgcagag
     1621 catattccgg gagcacgctt catcgggtac gaggcgggcg gccacttgtg ggtgggccat
     1681 cagcaagaga tgacggacga gattgcgagt ttcttgaagt catgatgcgg agcaagacga
     1741 aaaggtatcg aggaaccatt tccggcgcgt gacatggacc agacttattt gctcgtcttg
     1801 caaccccacc gaaacaaggc tgatgacatc ccgccgacaa gggcgaagaa gaccatcggc
     1861 tgccatttcc cgtgaaggat actgagggcg gctaaggcaa caaccagtag ccagcttttg
     1921 acgaatttcc acagacgcgc ttgcacggga agctcctccg ctgttcccga cgtccgccct
     1981 accgctattg aagcgagtgc gccaaggaca gccgcggcat acaaccacgc ctctctttca
     2041 ggcatcatcg agacaatgag tcccaaggca atgccgaacg gcgtccatgc aatcagtcgg
     2101 taagagtgtg tccgcgcatg caccggctgc ttactgttta cttaacggaa agctgatgac
     2161 cagtcctccc atgacgtcac cgaccttaaa atctttcttc ggactgctgg gatgcgtgtt
     2221 gtgacattca acgcacgccg acgtgacggc aatgtcggca tagaccgctt caaagacctg
     2281 ttccgcgctg gccggcacag tacttgcgct ggaataccgt cgattgggac gggcctgcac
     2341 ttccttcaga ccccgccgct cgaattcggt gcctgccgca ttggccttat tgatcggcca
     2401 ttgactgatc aagcggtagc ggacattcgg gccggtcaat tcggcaatct cgctcgtctc
     2461 tcgcaccagc tgtacgggta acgggagcga atgcgtcgtc cgccagtttt ccgagacatc
     2521 gacaatccgc tcttggcgaa gccgattggc gatgtgaatg gtgtaaaaat ggcggtgggc
     2581 cataatgacg gcgcgcaaat agtcagccac ggtttcggga ggcacgccta ccggccctga
     2641 ttgtttctca acggcgaaga tccccggcgc acccagcgcc gccatcgttg tcatcacggc
     2701 aatccaagtc acagttttcc gggtcatgat gccctctctt tcctctcgat ggggacgtga
     2761 ctcacaggta acggaaccga gtcaaatccg tatttcacca acagtttttg tatgcgaggc
     2821 gtcatcaaaa aagaagagaa ctgctccgca gtcgagcgca acgcaggtcg acaggttgag
     2881 gcgaccgctt gcccgaactg aaccggcacc aacacgccga agggatcttc atcgctgatg
     2941 cggacatagc cgctgttgat cgaatcgacg cgatagacca g
//
LOCUS       DASSCQ010000193        21927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_359639, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000193 DASSCQ010000000
VERSION     DASSCQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 21927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21927
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359639"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFH05_12425"
     CDS             complement(<1..578)
                     /locus_tag="VFH05_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6679072.1"
                     /translation="MQSDSRSRVGQCRSERRVGGAVTPLRAGVIGVGHLGQHHARLYA
                     SLPGSRLVAVNDQSAERAHTIADRHGARVCKSLDELLPLVDVVSVAVPTSSHYAVAKA
                     CLLAGKHVLVEKPIAVTMAEARELVRLAEDKHCRLQVGHSERFNPVMQVMRPHIGRPV
                     FIECHRLSSFSERGTDVDVILDLMIHDLDMVLS"
     gene            complement(512..1375)
                     /gene="lpxI"
                     /locus_tag="VFH05_12430"
     CDS             complement(512..1375)
                     /gene="lpxI"
                     /locus_tag="VFH05_12430"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET6679073.1"
                     /translation="MTQAPLVGGGERLGLIAGNGRFPIIFADNARKLGYQVSAVAHEG
                     ETDPELANHVDRIHWVKIGQLNKLIHAFKEDGVHHAVMLGGIKKTHVFTTVRPDFRTL
                     ALATRLALWKDDDILREFARELEGEGITICESTFGLEGILAEEGTLTSRKPTEKEWED
                     IRYGWDVAQEIGRLDIGQCVVVKDRMVVAVEAVEGTDEAIKRGGQLAKGGAVVVKRCK
                     PQQDLRFDLPAVGPRTIDVMASVSAAVLAVEAGRTILLDREMTLQKAQSSRIAVVGLV
                     NADQSGGSEAR"
     gene            complement(1379..2185)
                     /gene="lpxA"
                     /locus_tag="VFH05_12435"
     CDS             complement(1379..2185)
                     /gene="lpxA"
                     /locus_tag="VFH05_12435"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET6679074.1"
                     /translation="MEIHPQAIVHPKATLADDVSIGPFCVVGEHVTIGPGTRLLSHVN
                     VEGWTKIGARCELHPFVSMGGPPQHLGYQGEPTQVVVGDDNIFREYVTVNRATVQGGG
                     ITSIGDKNFLMAYCHVAHDCRLGSHLIMANAASLAGHIAISDHAIIGGLTGVHQYVRI
                     GAYAMVGGCCAVTQDVPPFMRVAGGYPARMYGLNSVGLRRHGFSAERIAVLKRAYDIL
                     FRSGHRMAEAAKLAKREFGDQPDVMAVLAFLEGTKRGICRSVGKDQADEE"
     gene            complement(2255..2722)
                     /gene="fabZ"
                     /locus_tag="VFH05_12440"
     CDS             complement(2255..2722)
                     /gene="fabZ"
                     /locus_tag="VFH05_12440"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET6679075.1"
                     /translation="MAVMEQAEIQALLPHRYPFLLVDRVLELEPDRRIVGIKNVTVNE
                     PFFQGHFPGRPVMPGVLIVEAMAQVGGLLAFKSLGPAAKPLVYLTGIDGAKFRKPVVP
                     GDRLRIEIDVLKKRSPFWKMLGKAFVESEVVCEAEVTAMVSEDKSAGGGPSAM"
     gene            complement(2942..3478)
                     /locus_tag="VFH05_12445"
     CDS             complement(2942..3478)
                     /locus_tag="VFH05_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6679076.1"
                     /translation="MKRNRRVFLASIGSLWWIVSSMALAADPVKVGVMDQQLVIEKSK
                     AGKRALEELKAYSATRQKIINSDDQELKELEQTIQDAKLSEAAKQEKQNQFQAKLEAY
                     QRRLAEFNREIQQKQREMVTEYSKKVQEAAQAVAQREGYTAVIDRGSEAAIRIVIYHQ
                     PALDVTEHVVKEFDKQNK"
     gene            complement(3513..4070)
                     /locus_tag="VFH05_12450"
     CDS             complement(3513..4070)
                     /locus_tag="VFH05_12450"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET6679077.1"
                     /translation="MRIEWPWVLARRLWVFSLAMWIATAFGCAGAGPKIDGKIGLVDP
                     GRVLNDSNAGKKAKESLTAFSKNRQALIEIEEKELRRMEEDFVKQASVLSPAAKNERE
                     QVFRRRMAEYQQKAAELNREVQEKQKDVLEAFRDKVEAVVAKVAKRNGLQVVIDKGKG
                     GPAIFGAEELDLTNQVIEEFNREYP"
     gene            complement(4101..6398)
                     /gene="bamA"
                     /locus_tag="VFH05_12455"
     CDS             complement(4101..6398)
                     /gene="bamA"
                     /locus_tag="VFH05_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET6679078.1"
                     /translation="MQRTRWLLVVLGLVLLTAGGVYEAPAQAPTAKVASIDIRGNQKI
                     ELPAIQGRLTLKPGDAYSPENVRGQIKILYDTGYFEDVQAETESTSDGIALVFVVREK
                     PFITEIVFDGNEELSDDKLKEKITIKSQAFLDQQQAKESAEKIRLAYQEDGYFSAQVI
                     PIVQTLDADRKRLTFFIKEGEKAKVKTVNFEGMRAASKAEIFKVTATREWVPWYGLVT
                     QLKLPSFLSDAGVLKREEMNNDVERIREVLLNKGYLNAQVGLPSVELSDDKKWFVVTY
                     AVVEGEPFTVAEIGFRGNTVFEDPELRDKMKIKPGEIFQRQKIRDEIGRLTDLYGSKG
                     YAFAEVIPNVNPNPEERTATIILNIKEGEMMRIRQINVHGNDKTKDNVIRRELRVDEQ
                     DVIDTPSLKRSFQRLNNLNFFETVEILPQQVDADKVDLNVRVKEKPTGQFSIGGGFST
                     LDKLVAIADITEGNLGGNGWMGRIRGQLGQQRTLGLITFRNPYLNDSLTSLQLDIYRS
                     MTNYITYFEEKSGASITFGRWLSEYVTGSISFVAEELNFSDPTIDAPEIILSQLGKQT
                     TTGFRTTLARDTRDYFLDPRSGLRTAVGFDFGTPYLGGTNNFYKYYLDAIKYTPLPFD
                     TRFSIHARYGVADGIDGKPIPLTERFFVGGINTMRGFVFGRAGPVTPSGSLIGAAKEL
                     IFNNDFIFTISSEAKLNGVIFFDWGKGFDDNEAISFGALRKAAGLEGRWISPFGPLRA
                     AYGINLDPREGERKGVFEFTIGSLF"
     gene            6606..7646
                     /gene="galU"
                     /locus_tag="VFH05_12460"
     CDS             6606..7646
                     /gene="galU"
                     /locus_tag="VFH05_12460"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET6679079.1"
                     /translation="MKNCRIIWGKYFNVKPIWSGTPRRRNPRADWRFLDITYPITYYR
                     RMSRADVRKAILPAAGLGTRFLPATKASPKEMLPLVDKPLIQYAVEEAVASGIEDIIV
                     ITGRGKRAIEDHFDRSVELEENLKGNGKGQLLSQIRHISNLANFCYVRQSEALGLGHA
                     VLCAQHLIGDEPFAVMLGDEIIDASVPALAQLIHVYKKRHGAVLGIQEVPHQEVNRYG
                     IISPDRLSDGLYKVTDLVEKPAPSDAPSNLAVIGRYVLPPEIFPILRKTKPGKNGEIQ
                     LTDALRELAKSSPMYALEVKGNRYDAGDKLGFLIATVEFALKNPSLGSDFAEYLRNRM
                     ESAGEQRRTSRA"
     gene            7696..10197
                     /gene="lon"
                     /locus_tag="VFH05_12465"
     CDS             7696..10197
                     /gene="lon"
                     /locus_tag="VFH05_12465"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6679080.1"
                     /translation="MSESIEQDLTPPQNVEIPDQLPLLPVRDIVVFPYMVLPLFVGRE
                     MSIKAIEAALAGNRMIFLATQKALDVENPAPDDIHSIGTVGIIMRMLKLPDERIKILV
                     QGLSKAKISGYIQTEPYYSVRIDKITEAKAPSSTLEIEAVMRTVKEQIERIVSLGKVL
                     IPDVMVVIENLEDPGRLADMIASNLGLKVEVTQSVLEVTDPLKRLRQISEILAKEIEV
                     LSMQQKIQAQAKGEMDKTQREYFLREQLKAIQKELGELDERAEEVAEFRKRVKDAKMP
                     EKVLKETEKQLKRLEKMHPDTAESATVRTYLEWMVELPWSKKSKDNLDLKAAHKVLNE
                     DHYDLEKVKERILEYLAVRKLKEKMKGPILCFVGPPGVGKTSLGKSIARALGREFVRI
                     SLGGVRDEAEIRGHRRTYVGALPGRMIQGMKQAGTSNPVFMLDEIDKVGMDFRGDPSA
                     ALLEVLDPEQNNTFTDHYLGVPFDLSEVMFITTANLIDPILPALRDRMEIIEIPGYTE
                     EEKLGIAQRYLIPRQLNEHGITEKHVKIAEAAIRQIIMHYTREAGVRNLEREIANVMR
                     KVAKKVAEGKGLGFPVNAGNLHKYLGVPKFVPESELEKDEIGVATGLAWTESGGDVLY
                     IEATVMKGKGVLTLTGHLGDVMKESAQAALSYVRSREKSLGINPDMFTKQDLHIHVPA
                     GAIPKDGPSAGITMATAIASALAQVPARRDLAMTGEITLRGRVLPIGGLKEKILAAKR
                     AKLTTVILPKRNKKDLDEIPKHILKGIQLVFADTMDDVMKVALRRRQPAKKKTGQPQA
                     PQEPTIPPTRIDKSRRAERTAHARPARLHTAPLLR"
     gene            10199..11248
                     /gene="thiL"
                     /locus_tag="VFH05_12470"
     CDS             10199..11248
                     /gene="thiL"
                     /locus_tag="VFH05_12470"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET6679081.1"
                     /translation="MASRPWRKAISTINEFDLIRALQRRYRINGPGVLRGIGDDAAVI
                     ASEPRRRLLFTTDLLAEGIHFDLATASFVDLGFRAATANLSDIAAMGGTPEYILVSLA
                     MPQSASKVQVEQMYRGLMTACRPHGVRLIGGDTSASATGWFLNITLVGSAPARDVLLR
                     SGSKPGDTLYVTGTLGDSRAGLQLLQHSPRRSRPSISFRHRRFLVTRHLRPTARIREG
                     CWLARGRWATSAIDISDGLSGDLRHLCDASQVGVDLDLSMLPISPACRAYAHGLNQDP
                     AMVALAGGEDYELLFTVPSRRSARFEQATRAQRIHVTRVGSMLPRGRGLHMIMPSGRR
                     QPLPVVSYEHFRSQR"
     gene            11264..11743
                     /locus_tag="VFH05_12475"
     CDS             11264..11743
                     /locus_tag="VFH05_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET6679082.1"
                     /translation="MASFRSLLRQVLHLEESPQRTALAFAVGAAIAFCPLYGFHMVLV
                     GICSWAFRLNFVALLAGAFINNPWTLVPVLGATYWIGAVILGRSDRPSFDWNDLGFRA
                     IYEQVMPYAVPFAVGGMVLSLVAAIVSYPLALYFITKHRQQTSARQVEPLPPADPLR"
     gene            11759..13186
                     /locus_tag="VFH05_12480"
     CDS             11759..13186
                     /locus_tag="VFH05_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6679083.1"
                     /translation="MARQEPTRQFNAPYDGSALIADPIHKYVSFTVPSSHADPHEQTE
                     KDLIDSPWVQRLRHIYQLQSARWVYPSAEHTRFVHSIGTMHVAGRFARHLYPFLKKST
                     PDVPSANYVEEFLRVTALVHDIGHGPFCHFFDDNYLHALHASHEKLGQLIIRDHLGPI
                     IRKIRRSPSGPFAKGEELHPDQIAHVILKEKGKDNSRLPRWLNMLQPVISGSYTADNL
                     DYVLRDSYMCGVAVGPVDLTRLIHYTIVTEKGFTIHKTGLPALQMFLNTRMYLYSNVY
                     FHRTTRAIDIHLRDIFGDTMKLLFPHDPRKKMDAYLTLTDSSLLETVRGWKTSRHNGE
                     RRLGEEWARILGRDVKWKMAYSTVLKEKGQERGMDFPSHSHFEKQIRKELPKHLQQVE
                     FRVDMAPLDPRPDPKDQRGNPLYVYDPGTGTVSTEPLEEFLDLLPTRLIQFRIYSLDH
                     RHDAVLSRAAGTVLNKTPSSIESHY"
     gene            complement(13271..14281)
                     /locus_tag="VFH05_12485"
     CDS             complement(13271..14281)
                     /locus_tag="VFH05_12485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6679084.1"
                     /translation="MIIPALKQVSQRVSRLDRMLITVIVLLTSVFPVELFPNAPPLPK
                     GIDGQYSGRQGQVVLVKVAVREEPTDVTGTFLDRTIPFFREMRTGEPSGYLGLVGIDM
                     QDAPGTYELAVTVKYGEKVQRLSYHVLVSKEKFAVEHLKLPKDKVDLDEKTLTRWKAE
                     QEQVRRALADNSALRLWQSSFVEPVNGKRTGIFGSVRIMNGQPRNPHNGEDIGAPLGA
                     DVAATNDGVVRLVVDHVFSGRGVFVDHGLGFYSMYFHLSEVLVKEGDLIQAGQIIGKV
                     GATGRATGPHLHWGVKLNGARVNPYALLQLPFPSRSLPGATSVAPPPSSEISPEALPV
                     KE"
     gene            complement(14364..15758)
                     /gene="pafA"
                     /locus_tag="VFH05_12490"
     CDS             complement(14364..15758)
                     /gene="pafA"
                     /locus_tag="VFH05_12490"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HET6679085.1"
                     /translation="MPSSGPMKQRIFGLENEYGLIFSPNGRIYLPMEKVLGYIFEGLI
                     PNSWPSNAFLVNGARFYQDTGCHPEYSTPECDNILDLVVHDKAGERLLEACLPAAEER
                     LREEGLSGEIYIFKNNTDSLGNTYGCHENFLMRRDVDFWKVTEQLIPFFVTRQIYSGA
                     GKVLKVSGKPQFFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAERYRRLHIIVGD
                     SNMSELVTYLKVGTATLVLSMIEEGFSVHGMELEDPVKAIREISRDPTVKRKVKLDDG
                     RQMTAVEIQRVYLERAHDYLTQQDHDPILDDVWKRWATILDKLEEDPMQLVREIDWIT
                     KRHLIQSYVDKKGCSWDDPRVFLLDLQFHDVKRTRGLYYLMESRGLVERVVEEDAVQR
                     AMSTPPQTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRV
                     DELVSQVSGLRFYR"
     gene            complement(15834..16517)
                     /gene="prcA"
                     /locus_tag="VFH05_12495"
     CDS             complement(15834..16517)
                     /gene="prcA"
                     /locus_tag="VFH05_12495"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HET6679086.1"
                     /translation="MPMPYYVSPEQMMQDKAEYAKKGIAKGRSIIAMEYVDGILLAAE
                     NPSASLHKISEIYDSIAFAGAGKYSEFENLRKAGIRHADLKGFMYSREDVTARSLANG
                     YSQSLGTIFSQEMKPLEVEILVVQIGHNGHSNEMYRISFDGSIIDEKQFAVIGGKSEA
                     VQAALKEKVPAKPPDLKAALNLSILALEQTGNQKLSLETLEVAVLDRTRENRKFRRYT
                     PAETKQFLA"
     gene            complement(16520..17347)
                     /gene="prcB"
                     /locus_tag="VFH05_12500"
     CDS             complement(16520..17347)
                     /gene="prcB"
                     /locus_tag="VFH05_12500"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HET6679087.1"
                     /translation="MKLPFLPNHDDASFFDFLTRQHPELTPGGRRPPEPPLPVHELPR
                     AVSPVIVPQATTVLAVKSQAGVVIAGDRRATEGFQIAERRIEKVFKIDDYSAMAIAGA
                     AGPCIEMAKLFQTELEHYEKLEGMQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYVG
                     YDLKRGEGRIFKYDLAGGRYEESDYHAIGSGGKDARNTMREHFKKAMTEQDAIKLALL
                     ALYNAADDDVGTGGPDLVRGIYPTAKIVNGQGITDVSESEIRSVYEAMLATRIAKER"
     gene            complement(17347..18846)
                     /gene="dop"
                     /locus_tag="VFH05_12505"
     CDS             complement(17347..18846)
                     /gene="dop"
                     /locus_tag="VFH05_12505"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HET6679088.1"
                     /translation="MRDSTPLTTPRVLGTETEFGIASRDASAVDPISNSLAVIGHYPG
                     ISVPHAVWDYENENPLLDARGFEVEGERERPNPDYNRQLNKVLANGGRLYVDGAHPEY
                     STPECTNPREVVAYERISERILGQCLDNMARLRGRDQFVLYKNNSDGKGNSYGYHENY
                     MLSRSVPFERIVKTLAPFFVTRLIYAGAGKVGAENQTSFTEFQISQRADFFECLVDLN
                     TMVKRPIVNSRDEPHADYGKYRRLHVIVGDANMAELSTYLKVGTLGIVLDLLEAGVDV
                     PDIELDDPVMGIKQVSRDLTMKGTLRMTDGRSTTAVEIQRAYLKSAMNFYACHELDQV
                     TKDVLVRWEDVLDKLERDPRSLAGELDWAAKRHMIESYMDRKGCGWDDPRVRLMDLQY
                     HDIRPDRGLYYTLERSHLIERVVQDVEVIRAEFTPPSGTRAYFRGRCIGKFSKSIYGA
                     SWTSVLFDVGNTTIKKVPLMDPLRGTESLTAELLDQSDTAEALLSKLKA"
     gene            complement(18853..20622)
                     /gene="arc"
                     /locus_tag="VFH05_12510"
     CDS             complement(18853..20622)
                     /gene="arc"
                     /locus_tag="VFH05_12510"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HET6679089.1"
                     /translation="MADTKGRPSRLRSLRDSVKHITKRLSEGDPDVIHRNDEQAREIE
                     KLRVQIQSMEEEIRRLYQSRYQLDQANKQNEKLVNTLQEAKAQIEALRAEVEKLTAPP
                     STYAIFSSLNQDGTGNVYVSGRKMKVSLHPSLMGKGLRKGQEVILNEALNVIEARGFD
                     VQGEVVRLKDLLEGNRALVTLHFDEEKVAELGDPLLAERLSVGDHLLFDPRSGCVIEK
                     LPKSEAEELVLEEVPDVDYANIGGLQHELEQVRDAVELPFLHPHLFAEYQLSAPKGVL
                     LYGPPGCGKTLIAKAVANSIAKKLGHLTGKEVRSYFLHVKGPELLNKYVGESERQVRE
                     VFKKAKERAADGNPVIVFFDEMDALFRTRGTGISSDIESTIVPQFLSEIDGVERLRNV
                     IVIGASNRQDLIDPAVLRAGRLDVKVKVGRPDAVAAKDIFSKYVGTSLPFDEDELKRH
                     GGDPKALVEHLTDLTVGAMYATSEENKFIEVTYANGEKEILYFKDFASGALIEGIVSR
                     AKKYAVKRALAQEGRGLRSDDLIRAIREEFKEHEDLPNTTNPDDWAKVAGKKGEKIVH
                     IRTISGGPAESRQIETITTGHYL"
     gene            complement(20756..21388)
                     /gene="thiE"
                     /locus_tag="VFH05_12515"
     CDS             complement(20756..21388)
                     /gene="thiE"
                     /locus_tag="VFH05_12515"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6679090.1"
                     /translation="MAALESRLLVITDRQHTKGRPLLSVLAQALRAGNIAIQLRERDL
                     CAKDLLTLASEIQQLMEPHNGQLLINDRLDVALSLEEAGVHLRSNSLPVGVVRRLLPP
                     LRLLGASAHSVAEALEVEANGADYVVLGPVYDTPSKRLFGPPLGLSILEEAARAVRLP
                     IFAIGGVTAGRARELRRAGAFGVAVITAILCADDVEAATTELLEAVNAPV"
     gene            complement(21648..>21927)
                     /locus_tag="VFH05_12520"
     CDS             complement(21648..>21927)
                     /locus_tag="VFH05_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET6679091.1"
                     /translation="IRNPYNLKIIMETVKVPIVVDAGVGTASDAALAMEYGADAVLMN
                     TAIAGAQDPIAMAEAMKYAVHAGRLAYKAGRIPRKLYATASSPIEGML"
BASE COUNT         4549 a         6711 c         5907 g         4760 t
ORIGIN      
        1 gagagcacca tgtccaaatc atggatcatg agatccagaa tcacgtcgac gtcggtgccg
       61 cgctcactga agctgctcaa gcgatgacat tcgatgaaga ccgggcgccc gatatgcggc
      121 cgcatcacct gcatgacagg attgaaccgc tcgctgtgtc cgacttgcaa ccggcaatgc
      181 ttgtcctccg ccagccggac cagttcccgg gcttctgcca tcgtgacagc gatcggcttc
      241 tccaccagca catgcttgcc ggccagcaga cacgctttcg cgacggcgta atgcgaggat
      301 gtcggcacgg cgacactgac gacatcgacg agaggcagga gttcgtcaag ggatttgcag
      361 acgcgggccc cgtgtcggtc cgcaatcgtg tgagcccgct cagcagattg gtcgttcacg
      421 gccacaaggc gcgacccggg aagcgaggcg tagaggcgtg cgtgatgctg gccgagatgt
      481 cctactccga tcacacccgc tctcaacggt gtcaccgcgc ctccgaccct ccgctctgat
      541 ctgcattgac caaccctact acggctatcc gactggattg cgccttctgc aaggtcattt
      601 cgcgatccag caggatggtg cgtccggcct ccacggccaa cacggctgca ctgactgatg
      661 ccataacatc gatggtccgg ggtccgaccg ccggtaggtc gaatcgtaaa tcttgctgag
      721 gcttacaacg tttgacgacg acggccccgc ccttggccaa ttgcccgccg cgtttgatcg
      781 cttcgtcggt accctcaacg gcttccacgg cgacgaccat gcggtctttg acgacgacgc
      841 attgcccgat gtcgaggcgg ccgatctctt gcgcgacgtc ccatccatag cgaatgtctt
      901 cccactcttt ttctgtgggt ttccggctgg tgagcgtccc ctcttccgcg agaatccctt
      961 caagaccgaa ggtcgattca catatcgtga tgccttctcc ttcgagctcc ctggcgaact
     1021 cccgcaggat gtcgtcgtct ttccacagag ccaatcgagt ggccagcgcc aaggtgcgga
     1081 aatctggacg cacagtcgta aatacgtggg ttttcttaat gccgccgagc atgacggcat
     1141 gatgcacgcc gtcttcctta aaggcatgaa tgagcttatt gagttggccg atcttgaccc
     1201 agtggatacg atcgacatgg ttcgccaact cgggatctgt ttctccttcg tgcgcaacag
     1261 ccgaaacctg gtatcccagc ttcctcgcat tgtccgcaaa gataatggga aaccggccgt
     1321 tgccggcaat caaacccagc cgctcaccgc cgccgactag aggcgcctgc gtcaccggct
     1381 actcctcgtc tgcctggtct ttaccgacgg aacggcagat cccccgcttg gttccctcca
     1441 ggaaggccag tacggccatc acgtccggct gatcgccgaa ctcccgtttg gcaagtttgg
     1501 ccgcttcggc catccggtgc cccgaccgga acaaaatgtc gtacgcgcgc ttgaggaccg
     1561 caatccgctc ggccgagaac ccatgccggc gcaatccgac ggaattcaac ccgtacatcc
     1621 gggcgggata tcctccggcc acacgcatga acggcggaac gtcttgagtc acggcgcagc
     1681 agccgcccac catcgcgtag gcgccgattc ggacgtattg atgcacgccg gtaagcccgc
     1741 cgatgatcgc atgatcgctg attgcgatgt gccctgccag gctggctgcg ttcgccatga
     1801 tcagatggct gcccagacga cagtcgtgcg ccacatggca ataggccatg agaaaattct
     1861 tgtcgccgat agacgtgatt ccgccgccct gcaccgtcgc gcgattgacg gttacatact
     1921 cgcggaaaat gttgtcatcc cccaccacga cctgagtcgg ctcgccctga taccccaaat
     1981 gctgaggcgg cccgcccatc gacacgaagg ggtggagctc gcaacgcgca ccgatcttgg
     2041 tccagccttc gacgttgaca tgggagagca gacgggttcc tggtccaatg gtgacatgtt
     2101 ctccgacaac gcaaaacggt ccgatgctca cgtcatcggc gagcgtggcc ttgggatgga
     2161 caatggcttg tggatgaatc tccacgcagg acctcctcct agggacgata cgtcagcgac
     2221 cggtctcgct cctcccaacc cttctcaacg ccggtcacat ggccgatgga ccgccaccgg
     2281 cgcttttgtc ttccgacacc atggcggtga cttccgcttc gcagacgacc tccgactcca
     2341 cgaaggcttt gcccaacatt ttccagaatg gagaccgttt tttgaggaca tcgatttcaa
     2401 tgcgaagacg gtcgcccggc accaccggct tgcgaaattt tgccccgtca atgccagtca
     2461 aatagacgag aggcttcgcc gccggcccca acgatttgaa ggccaagagt ccgccgacct
     2521 gcgccatcgc ttccacgatc aggacaccgg gcatgaccgg ccgtcccggg aaatgtcctt
     2581 ggaagaacgg ctcatttacc gtcacgttct taatgccgac tatccgacga tccggttcga
     2641 gttcgaggac ccggtcgacg agcaaaaacg gataacgatg aggtaagagg gcctggatct
     2701 cggcttgttc catgacggcc atcgcggaac ctccaaatgg caagcgtgcg cgttacatct
     2761 ctctcgcgcg ggctgcctgg gtcctgctgc cacccaccca accctgcgca tcttgtgttc
     2821 ccgccgaccc accgttgctt cgcaagcgat tcctcgacgt gcgctggact cagacgagcg
     2881 tcgcctgttc gactctcgcg tgcacgtggg gcagtaccag atgcgagcgg cctgcgccac
     2941 cctatttgtt ctgcttatcg aattctttca cgacgtgttc ggtcacatcc aaggccggct
     3001 ggtgatagat cacaatcctg atggcggctt cactgcctcg gtcgatcacg gcggtgtagc
     3061 cctcccgttg cgcgaccgct tgcgcggcct cttggacctt cttggagtac tcggtcacca
     3121 tttcgcgctg cttctgctgg atctcccgat tgaattcagc cagacggcgc tgatacgcct
     3181 cgagcttcgc ctggaactgg ttctgcttct cctgcttggc cgcctcgctc agtttcgcat
     3241 cctggatcgt ctgctccagc tctttgagtt cctgatcgtc tgagttgatg atcttctgcc
     3301 tcgtcgcgga ataggccttt aactcctcga gggcacgttt gcctgctttg gacttctcaa
     3361 tcacgagttg ctggtccatg acaccgacct tcaccggatc cgccgccagc gccatcgacg
     3421 acacgatcca ccacagggag ccgatacttg ccaaaaagac tcgtctattc cgcttcatga
     3481 atactcctcc tcgaccatcc ctcgatcagg cctcacgggt attctctgtt gaactcttcg
     3541 atgacttgat tcgtgagatc caactcttcg gccccgaaaa tcgcagggcc tcctttgcct
     3601 ttatcgatga cgacttgcaa gccattcctc ttcgccacct tggcgacgac ggcctccact
     3661 ttatcacgga acgcttcgag aacgtccttt tgtttttcct gcacttctcg attcagctca
     3721 gccgctttct gctgatactc cgccatacgt cgtctgaaaa cctgttcccg ttcgttcttc
     3781 gcggccggac tgagcacgct ggcctgtttg acgaaatctt cctccatgcg ccgaagctct
     3841 ttctcctcga tctcgatcag cgcctggcga ttcttggaga acgcagtcag cgattctttc
     3901 gcctttttcc cggcattcga gtcgttgagc acgcgtcccg gatcgacgag tccgattttt
     3961 ccgtcgatct tgggaccggc ccccgcacag ccgaaggctg tggcgatcca catggccagt
     4021 gagaataccc acagcctgcg tgccaatacc catggccact caatgcgcac tcctgggctc
     4081 acctttcacc tcgacacgtg ttagaacaac gacccgatcg tgaactcaaa cacgcctttc
     4141 cgctcgcctt cgcgaggatc cagattgata ccgtatgcag cgcgcagcgg gccgaacggg
     4201 gagatccacc gtccctccag accggccgct ttgcgcaatg caccaaagga aatcgcttcg
     4261 ttgtcatcaa accctttccc ccagtcgaaa aaaatcacac cgttcaattt tgcctcggag
     4321 gaaatcgtga aaatgaaatc gttgttgaaa atgagctcct tggcggcacc gatcagcgaa
     4381 ccgctgggcg tcaccgggcc cgcccgaccg aagacgaatc cgcgcatcgt attgatgccg
     4441 ccgacaaaaa accgttctgt caacggaatg ggtttcccat ctattccgtc ggctacacca
     4501 tagcgggcat ggatggagaa tcgtgtatcg aacggcagcg gcgtatattt gatggcatca
     4561 aggtaatatt tatagaagtt gttcgtgcct cccaaatagg gcgtcccgaa atcgaaaccc
     4621 acggcagtcc gcagaccgct tcgggggtcc agaaaataat cccgtgtgtc gcgcgccagc
     4681 gtcgtgcgga atccggtcgt ggtttgcttg cccaattggc tcagaatgat ctcaggcgca
     4741 tcgatcgtcg gatcgctgaa attcaactct tccgcgacga agctgatgct tcccgtcaca
     4801 tattcggaca gccaccggcc gaaggtaatg ctcgcgcccg atttctcttc gaagtacgtg
     4861 atgtagttgg tcatgctgcg gtagatgtcc aactgcaggg aggtcagcga atcgttcaag
     4921 tacggatttc gaaacgtgat caagcccaag gtccgctgct ggccgagctg tccacgaatc
     4981 cggcccatcc atccgttgcc cccgagattt ccttcagtga tatcggcgat ggccaccagt
     5041 ttgtccaaag tactgaatcc gccgccgata ctgaactgcc ctgtcggttt ttcttttacc
     5101 ctgacgttca agtccacctt gtccgcgtcc acctgctgcg gcaggatttc gaccgtttcg
     5161 aagaagttga gattattcag gcgctgaaag cttcgcttga gcgacggcgt gtcgatgacg
     5221 tcctgttcgt cgacgcgcaa ctcgcgtcgg atgacgttgt ctttggtctt gtcgttgcca
     5281 tgaacattga tctgccggat gcgcatcatc tcgccttcct tgatattgag aatgatggtc
     5341 gcggtgcgtt cttcgggatt cggattcacg ttgggaatga cctccgcgaa ggcgtagcct
     5401 ttgctgccgt agagatcggt cagtcggccg atttcatcgc ggattttctg ccgctgaaag
     5461 atttctcctg gcttgatctt cattttgtcc cgtaactccg gatcttcgaa aaccgtattg
     5521 ccgcggaacc cgatctcggc cacggtgaac ggctctcctt cgacgacggc ataggtgacg
     5581 acgaaccatt ttttatcgtc gctcaactcc actgagggta agccaacctg ggcgttcaga
     5641 taccctttgt tgagcaacac ctccctgatc cgctcgacgt cattgttcat ttcctcgcgc
     5701 ttcaggacgc cggcatcgga gagaaaggag ggcagcttga gctgcgtcac caggccatac
     5761 cagggtaccc actcccgagt cgcggtcacc ttgaatatct cggccttgga cgcggcccgc
     5821 atcccctcga agtttacggt cttgaccttg gctttctcgc cttccttgat gaaaaaggtg
     5881 agccgcttac gatcggcatc caaggtctgg acgatcggga tgacttgggc gctgaaatag
     5941 ccgtcttctt gataggcaag gcgaattttt tccgcgctct ccttcgcctg ctgctgatcc
     6001 aaaaatgcct ggcttttgat cgtgatcttt tccttcagct tgtcgtcgct taattcctca
     6061 ttgccatcga agacgatttc ggtaatgaag ggtttctctc ggaccacgaa caccagggca
     6121 ataccgtccg aagtcgactc ggtctctgcc tgcacgtcct caaagtatcc ggtgtcatag
     6181 aggatcttga tctggccccg cacattctcg ggggagtagg catcgcccgg tttcaaagtc
     6241 aatcgaccct gaatggcggg aagctcgatt ttctggttac ctcgaatatc gatcgaggct
     6301 accttggctg tcggggcctg agccggcgcc tcatacaccc cacccgccgt caggagaacc
     6361 aggccgagca ccaccagcaa ccagcgagtg cgttgcacca gcgacaactc tctcactcgc
     6421 accagattcc cgaacgaacc acagagaata cagcttccat ctcaaggccc cgatcagtgg
     6481 agacatgacg cccttcacct gggcactgtc gccgcgcatc cgaagccgtc gatgacagca
     6541 gcaaggaagg ttgctgacgt gagatgccta cgcgtgcggc gttgcagcat cagccatgaa
     6601 cccgtgtgaa aaactgtcgg attatatggg gcaagtattt caatgtaaag ccgatctggt
     6661 ccggaactcc gcgccgacgt aaccctcgag ccgattggcg attccttgac atcacctacc
     6721 ccatcacata ctatcgccgt atgtctcgag ccgatgttcg taaagccatt ctgcccgccg
     6781 ccggtctcgg caccagattt ctgcccgcga cgaaagcgtc tccgaaagaa atgcttccgt
     6841 tggtcgacaa gccgctcatt caatacgcgg tagaagaggc cgtcgcctcg ggcatcgagg
     6901 acatcatcgt gatcaccgga cgaggcaagc gcgcaatcga agatcatttc gaccgttcag
     6961 tcgagctcga agaaaatctc aagggcaacg gcaaaggaca gctgctcagt caaatccggc
     7021 atatttccaa tctggctaat ttctgctacg tgcgacagtc cgaggcgctg ggtctgggac
     7081 atgccgtgct ctgcgctcag catttgatcg gcgatgaacc ctttgccgtc atgttgggtg
     7141 acgaaatcat cgacgcgtca gttcccgcgc tcgcgcaatt gattcacgtc tataagaagc
     7201 gtcacggcgc cgtcctgggg attcaggaag tcccgcatca ggaagtgaac cgctacggca
     7261 tcatttcacc ggatcgactc tcggacggtc tctacaaagt gacggatttg gtggaaaaac
     7321 cggccccatc cgatgcgccc tcgaatctgg ccgtcatcgg ccgctacgtc ttgcctccgg
     7381 agattttccc gattctccgg aagaccaagc ccgggaagaa cggggagatt caactgaccg
     7441 atgcgctgag agagttggcc aagagttcgc cgatgtatgc gctcgaggtg aagggcaacc
     7501 gctacgacgc gggggacaag ctgggctttc tgatcgccac agtggagttc gccttgaaaa
     7561 acccgtcatt aggctccgat ttcgccgaat atcttcgaaa ccggatggag tcggccggag
     7621 agcagcgccg caccagcaga gcctgaccat cgcatcgctt ctttcgaacg attcagtaga
     7681 gatcgtgaga cgccgatgag cgaatcgatc gaacaagacc tgacgcctcc tcagaacgtc
     7741 gagattcccg accaactgcc cctgcttccc gttcgcgata tcgtcgtatt tccctacatg
     7801 gtgcttccct tgttcgtcgg tcgagagatg tcgatcaagg cgattgaggc ggcgctcgcc
     7861 ggcaaccgca tgatcttcct cgccacgcag aaggcgctgg acgtcgaaaa tcccgctccc
     7921 gatgacattc attccatcgg cacggtcggc atcatcatgc gcatgctcaa gctgccggac
     7981 gagcggatca agatcttggt ccaaggactc tccaaggcca agatctccgg atacatccag
     8041 accgagccgt actactccgt gcggatcgac aagatcaccg aagcgaaggc cccaagttcg
     8101 actcttgaaa tcgaagccgt catgcgtacg gtgaaagaac agattgaacg gatcgtcagc
     8161 ctggggaagg tgctgattcc cgacgtgatg gtcgtcattg aaaatctgga agatcccggc
     8221 cgcctcgccg acatgatcgc ctcaaacctc ggcctcaaag tcgaagtgac gcagtcggtc
     8281 ctggaggtca cggacccgct gaagcgcctg aggcagatca gcgaaatcct cgccaaagag
     8341 attgaagtcc tgtccatgca acagaagatc caggcccagg ccaagggcga gatggacaag
     8401 acccaacgcg agtacttctt gcgcgagcaa ctcaaggcca tccagaaaga gctcggcgag
     8461 ctggatgaac gggcggagga agtcgccgaa tttcgtaagc gcgtcaaaga cgccaagatg
     8521 cccgagaaag tgttaaagga aacggaaaaa cagctgaaac ggctggaaaa gatgcacccg
     8581 gacacggcgg aatctgccac cgtccgcacg tatctcgagt ggatggtcga actcccgtgg
     8641 tccaaaaaat cgaaggacaa tctggatctc aaggctgcgc acaaggtctt gaacgaagac
     8701 cactacgacc tcgaaaaggt caaagagcgc attttggagt atctcgccgt ccggaagctg
     8761 aaagaaaaga tgaaggggcc gattctctgc tttgtcggcc cgcccggcgt cggcaagact
     8821 tcgcttggca agtcgatcgc ccgcgcgctg ggccgcgaat tcgtccgcat cagtctcggc
     8881 ggggtacgcg acgaagcgga aatccgcggg catcggcgga cctatgtcgg tgcgctgccc
     8941 ggacgtatga ttcaaggcat gaagcaggcc ggcaccagca atcctgtgtt catgctggac
     9001 gaaatcgaca aagtggggat ggacttccgg ggcgatcctt cggccgccct gctcgaggtg
     9061 ctcgacccag aacaaaacaa cacctttacc gatcattacc tgggcgttcc gttcgacctg
     9121 tcggaagtga tgtttatcac gaccgccaac ttgatcgacc ccattctgcc cgccctgcgc
     9181 gaccgcatgg agatcattga gattcccggc tacaccgagg aagaaaagct cggcatcgcg
     9241 cagcgctatc tgatcccccg gcaactcaac gagcacggca tcaccgaaaa gcacgtcaag
     9301 attgccgagg cggcgatccg ccaaattatc atgcattaca cgcgggaagc gggcgtccgg
     9361 aatctcgagc gcgaaattgc caatgtcatg cggaaagtgg cgaaaaaagt ggccgaaggg
     9421 aaaggactgg gctttccggt caatgccgga aatctccaca agtatctggg cgtccccaag
     9481 ttcgttccgg aatccgaact tgaaaaggac gagatcgggg tcgcgacggg attggcctgg
     9541 actgagtcgg gcggcgacgt cctgtatatc gaagccacgg tgatgaaggg caaaggcgta
     9601 ttgaccctga ccggacacct gggcgatgtc atgaaagaat cggcacaggc cgcgttgagc
     9661 tatgtgcgat cgcgcgaaaa gtctctgggg atcaaccccg acatgtttac caagcaggac
     9721 ttgcacatcc atgtcccggc cggcgccatt ccgaaagacg gaccgtcggc tggcatcacg
     9781 atggccaccg ccattgcatc ggcgctggcg caggtgccgg cacgccggga tctcgccatg
     9841 accggcgaaa tcacgttgcg cgggcgggtc ctgccgatcg gcggcttgaa ggaaaaaatt
     9901 cttgccgcga aacgggccaa gctcacaacg gtgattctgc ccaaacgcaa caagaaagat
     9961 ctcgacgaaa tcccaaaaca cattttaaaa ggcattcaac tcgtctttgc cgatacgatg
    10021 gacgacgtca tgaaagtggc cttgcggcgc cggcagccag ccaagaaaaa gaccggccag
    10081 cctcaagcgc cccaggaacc cacgataccg cccacacgaa ttgacaaatc gaggcgggcc
    10141 gagcggacag cccacgcacg accagctcgc ctccatacgg ctcctcttct ccggtagcat
    10201 ggcatctcgg ccctggcgca aggccatctc caccatcaac gaatttgacc tcattcgagc
    10261 gctgcagcga cggtatcgaa tcaatggccc tggagttctc cggggcatcg gcgatgatgc
    10321 cgccgtcatc gcctcggaac ctcgtcgcag actcttgttc acgaccgatc tgctggcgga
    10381 aggtatccat ttcgaccttg cgaccgccag ctttgtcgat ctagggtttc gtgcggcgac
    10441 ggccaatttg agcgatattg cggccatggg cggaacccct gaatacattc tggtctctct
    10501 cgccatgcct caatccgcct cgaaagtgca ggtcgaacag atgtatcgtg gtctcatgac
    10561 cgcctgccga ccccatggtg tcagactgat tggcggcgat acctctgcat cggcgacagg
    10621 ctggttcctc aacatcacgc tggtcgggtc ggccccggca cgcgatgtcc tgctccgcag
    10681 cggttccaag cccggcgaca ccctatacgt caccggcacc ttgggggatt cccgggcggg
    10741 actacagctc ttgcaacaca gccctcgaag gagccgaccc tccatttcgt tccgccatcg
    10801 acggttcttg gttaccagac atctgcgccc gactgcgcgc attcgggaag ggtgctggct
    10861 ggcaagagga cggtgggcga catcggccat cgatatctcc gacgggctct ccggcgatct
    10921 ccgacacctc tgcgacgcaa gccaagtcgg cgtcgatctc gatctttcga tgttgccgat
    10981 ttctcccgcc tgccgcgcct atgctcacgg cctcaatcag gatccggcga tggtcgcgtt
    11041 ggccggcgga gaagactacg aactcttgtt taccgtcccg tcgcgccgat ccgctcgttt
    11101 cgaacaggcg acgagggcac agcgtatcca tgtcacgcga gtcggaagca tgctccctcg
    11161 cggccgcggt ctccacatga tcatgcccag cgggcgtcgg cagccgttgc cggttgtcag
    11221 ttacgaacat ttccggtctc aacgctaatg taaacccact cccatggcct cttttcgatc
    11281 actgttaaga caggtgctgc atctcgagga atcgccccaa cgcacggcgc tcgcctttgc
    11341 agtgggcgcc gcgattgcgt tctgtccctt gtacggcttt cacatggtcc tggtaggcat
    11401 ttgtagctgg gctttcagat tgaattttgt ggccctgctg gcaggggcgt tcatcaataa
    11461 tccctggacg ctggttcccg tcctcggcgc cacctattgg atcggcgccg tcattctcgg
    11521 acgctccgac cggccttcgt tcgactggaa cgacctgggc ttccgcgcca tctatgaaca
    11581 ggtcatgccc tatgccgtcc ccttcgccgt tggcggcatg gtgctcagtc tggtggcggc
    11641 tattgtgtca taccctctgg ccttgtattt cattaccaag catcgacaac agacatcagc
    11701 gcgccaggtg gagccattgc cgcccgccga ccccctacga taagatgctg tcgcgcccat
    11761 ggcacgacag gagcccacac gccaattcaa cgctccctac gatggctcgg ccctgatcgc
    11821 tgatccgatt cacaagtatg tatccttcac ggtgccatcg agccatgccg atccgcacga
    11881 acaaaccgaa aaagatctca tcgattcccc ctgggttcaa cgcctccgtc atatatatca
    11941 actccaaagc gctcgctggg tctatccctc tgcggagcac acccgctttg tccattccat
    12001 cggcaccatg cacgtggccg gccggtttgc acgccatctc tacccgttcc tgaaaaaaag
    12061 cacgcccgat gtcccttcgg ccaactacgt cgaggaattt cttcgtgtca ccgcgcttgt
    12121 ccacgacatc ggtcacggcc ccttctgcca tttcttcgac gacaattact tgcacgccct
    12181 tcacgcgagc catgaaaaac tgggacagtt gatcattcgg gatcatctgg gcccgatcat
    12241 cagaaagatt cgccgcagtc catccgggcc gtttgccaag ggcgaagagc ttcaccccga
    12301 tcagatcgcc catgtcatct tgaaggaaaa gggcaaggat aactcgcgtc tcccccgctg
    12361 gctgaacatg ctgcagcccg tgatctcggg aagttacacg gccgataatc tggattacgt
    12421 cctccgtgac tcctacatgt gcggagtcgc cgtcggtccg gtcgacctga cccggctcat
    12481 tcattacacg attgtcacag agaaaggatt tacgattcac aaaacgggac ttccggcgct
    12541 gcaaatgttc ttgaacacca gaatgtacct ctactcgaac gtgtactttc accggacgac
    12601 gagagccatc gacatccatc ttcgcgacat tttcggggac actatgaagc tgctgtttcc
    12661 ccacgatccg cgcaagaaga tggatgcgta tctgacgctg accgattcct ccttgctcga
    12721 aacggtacgc gggtggaaaa catcccgtca caacggcgaa cggcgtctcg gcgaggaatg
    12781 ggcacgcatt ttaggacgtg acgtgaagtg gaaaatggcg tacagcacgg tgttgaagga
    12841 aaaggggcaa gaacggggaa tggacttccc gagccattcg cacttcgaaa agcagatccg
    12901 aaaggaactg cccaagcacc tgcagcaggt cgagtttcgt gtcgacatgg caccgctcga
    12961 tccacgcccg gaccccaagg accagcgggg caatcctctg tacgtgtacg accccggaac
    13021 cggtaccgtt tccaccgagc cgctcgaaga attcctcgat ttgttgccga cccggctgat
    13081 ccaattccgc atctattcgc tggatcaccg gcatgacgca gtgctttccc gcgccgccgg
    13141 gacggtgctc aacaaaaccc cgtcgagcat cgaatcacac tactaaacga gcgtggtaat
    13201 tcgtctgtgt gtccgtctgg aacaggcgcg atgatggatg tgtgcttcca caggcgtcgt
    13261 caccgacacg ctattctttg accggaagcg cttcgggaga gatttcggag gaaggcggcg
    13321 gagccaccga agtggcgcct ggcagagatc ggctgggaaa cggaagctgg agcagtgcgt
    13381 aggggttcac gcgggcgcca ttgagtttga caccccagtg caaatgcggg cctgtggcac
    13441 ggccggtggc tccaaccttg ccgatgattt gcccggcctg tatgagatct ccctctttca
    13501 cgagaacctc tgagagatgg aagtacatac tgtaaaaccc caacccatga tcgacgaaga
    13561 caccccggcc tgaaaatacg tgatccacga ccagccgtac cacgccatcg ttggtcgctg
    13621 ccacgtcagc tcccagcggg gcgccgatgt cctctccatt gtgggggttt cggggctgac
    13681 cattcatgat ccggacactg ccaaagatac ccgtgcgctt cccattcacc ggttccacga
    13741 aactgctttg ccagagacgc agtgccgaat tgtccgcgag agctcgccga acctgctcct
    13801 gctcggcctt ccagcgtgtc aaggtctttt cgtccagatc caccttgtcc ttcggtaatt
    13861 ttaggtgctc caccgcgaat ttttccttgc tcaccagcac atggtagctg aggcgctgta
    13921 ccttctcgcc atatttcacg gtaacggcga gctcgtaggt gccgggggca tcctgcatat
    13981 cgatgccgac gagccccaaa tagccagatg gctcgccggt tctcatttcc cggaaaaagg
    14041 gaattgtgcg gtcgagaaag gtgcctgtga cgtcagtcgg ctcttcccgg accgcaactt
    14101 tcaccagcac gacctgtccc tggcgcccgc tgtattgccc gtcaatgccc ttaggcaagg
    14161 gcggcgcatt cggaaacagt tcgaccggaa agacactggt cagcaacaca atgacggtga
    14221 tgagcatccg gtccagccga gaaacccgtt gactgacctg cttcaacgcg ggaatgatca
    14281 tcttttcctc gaacctacga ctcggacgga ctacacgcta ctcggtgtgc aacaaggccg
    14341 atgacagctt ttttacagct ctctcatcgg taaaaccgca gaccggacac ctgtgacacc
    14401 aactcatcga cccgccgatt gaccgcactg aacggatcca tgcagagaat cgtttcctcc
    14461 cagtagccgt tcagcttcag ataggtccaa tcgacggtgt aggaacgatt cttcgcccgg
    14521 gcaaatcgaa tgaagtcccc acgcactttt gccctggtcg tttgcggcgg cgtcgacatg
    14581 gcccgctgta cggcgtcttc ttcgaccacc cgctcgacca atccacgtga ctccatgagg
    14641 tagtacagcc ctctcgtgcg tttgacgtca tggaactgca aatcaaggag aaagacccgg
    14701 ggatcgtccc agctgcagcc cttcttatcg acataggact ggatcagatg ccgttttgtg
    14761 atccaatcga tttcacgcac gagttgcatg ggatcctctt cgagcttgtc cagaatcgtc
    14821 gcccatcgct tccagacatc gtccaaaatc ggatcgtgat cttgctgagt caggtagtca
    14881 tgcgcccgct ccaaatacac gcgttggatc tcgaccgccg tcatctgacg accgtcgtca
    14941 agtttaactt tgcgtttcac cgttgggtct cgggagattt cgcggatcgc cttgacggga
    15001 tcttccagtt ccataccgtg cacggaaaac ccttcctcga tcatcgagag cacgagcgtt
    15061 gcggtcccca ccttgaggta ggtgaccagt tccgacatgt tggaatctcc gacgatgata
    15121 tgcagccgcc gatagcgctc cgcatccgcg tgcggttcat cgcgcgtgtt gatgatgctg
    15181 cgcgaggaag tggtcgagga ggaggttttt tcgtgtatgt gttgcgcgcg ctgagaaatg
    15241 aaaaactgag gtttgccgga aaccttcaac actttccccg cgccgctgta aatctgccgg
    15301 gtgacaaaaa atgggatgag ctgctcggtc actttccaga aatccacatc ccgacgcatg
    15361 aggaaatttt catggcaccc gtaggtgttc cccagtgaat ccgtattatt tttgaaaatg
    15421 tagatctctc cggaaagccc ttcttcacgc agccgttctt cggccgccgg caaacaggcc
    15481 tccagcaatc gctcaccggc cttatcatgg acgacgagat cgagaatgtt gtcgcattca
    15541 ggggtggagt attccggatg gcagccggtg tcctgataga aacgcgcccc gttcaccagg
    15601 aacgcattgg atggccagct gttgggaatc aagccctcaa agatatagcc taggactttc
    15661 tccatgggca gatagatccg cccgttcggc gagaaaatga ggccatactc attttcaaga
    15721 ccgaagatcc gttgcttcat tggaccggaa gatggcataa gcggctcgag acagaataca
    15781 cggcctcccg cccatcttca acagaagcgg aaggattcaa catgcaggat tgattatgcc
    15841 aggaactgct tcgtttcggc gggggtgtac cgacggaact ttcggttttc tcgtgtgcgg
    15901 tccaacacgg ccacttccaa cgtctcgagc gacagtttct gattccccgt ctgctcaaga
    15961 gccaggatgg agagattcag cgccgctttc agatcaggcg gcttagctgg cactttttcc
    16021 ttgagcgccg cctgcacggc ctcggatttt ccaccgatga cggcaaactg cttctcatcg
    16081 atgatgctgc cgtcgaaaga aatccggtac atctcgttgc tatgcccgtt gtgcccgatc
    16141 tggaccacga gaatttcgac ctccagtggc ttcatctcct ggctgaaaat cgtcccaaga
    16201 ctttgcgagt agccattggc cagcgagcgg gccgtgacgt cctcacggct atacatgaag
    16261 cccttgaggt ccgcatgcct gatcccggcc ttccgcaaat tttcgaattc gctgtatttg
    16321 ccggcgccgg cgaaggcgat gctgtcataa atttccgata ttttgtgcaa gcttgcgctc
    16381 gggttctccg ccgcaagcaa aatgccgtcc acgtactcca tggcaatgat cgaacgcccc
    16441 ttggcgatgc ccttcttggc atactccgcc ttgtcctgca tcatctgctc gggcgagacg
    16501 tagtagggca tcggcatgtc tatcgctcct tcgcaatgcg ggtcgccagc atcgcctcgt
    16561 atacgcttcg gatctcggac tcggacacgt cggtaatccc ttgaccgttg acgatctttg
    16621 cggtgggata aatgcctctg accagatcgg ggccacccgt cccgacgtcg tcatccgccg
    16681 cattatagag ggccaggagc gccaacttga tggcatcttg ttcggtcatg gccttcttga
    16741 aatgctcgcg catcgtgttg cgggcatcct ttccgcctga cccgatcgcg tgatagtccg
    16801 attcttcgta gcgtccgccg gccagatcgt acttgaagat gcgtccctct ccccgcttga
    16861 ggtcgtatcc gacatacagc ggcatgacca ccaatccttg gaagaccatc ggaagattag
    16921 ccttgaccat ctgcccaagc ttgttggcct tcccttcgca agacagttgc atgccttcga
    16981 gcttctcgta atgctcaagc tcggtttgga agagtttcgc catctcgatg cacggtcccg
    17041 cggcaccggc gatcgccatg gccgagtagt cgtcgatctt aaagactttt tcaatccggc
    17101 gttcggcgat ctgaaagcct tccgttgcac ggcgatcccc ggcaatgacc acccctgctt
    17161 gcgacttgac cgccaacacg gtcgtcgcct gcgggacgat cacagggctc accgcccgcg
    17221 ggagctcatg aaccgggaga ggcggttcgg gcggccggcg tcctccgggc gtcagctcag
    17281 ggtgctgcct ggtcaggaag tcaaaaaaac tcgcgtcgtc gtggttggga aggaagggga
    17341 gcttcattat gccttcagct tggagagcaa cgcctctgcc gtgtccgact ggtcgagcaa
    17401 ttcggccgtc agagattccg tgccccgcaa gggatccatc agcggcacct tcttgatcgt
    17461 ggtattgccc acatcgaaga gcaccgacgt ccagctggcg ccatagatgg atttcgagaa
    17521 cttgccgata caccgtcccc ggaagtaggc acgcgtgccc gaaggcgggg tgaattcggc
    17581 gcggatgact tcgacatcct gcaccacccg ctcgatcaga tggctccgct ccagcgtata
    17641 gtagagtcct ctgtcaggcc ggatgtcatg atactgcagg tccataaggc gcacgcgtgg
    17701 atcgtcccac ccgcatccct ttcggtccat atacgactcg atcatgtgcc gcttggccgc
    17761 ccaatcaagt tctccggcca gcgatcgcgg atcccgttca agcttgtcca gcacgtcttc
    17821 ccaccggacc agaacgtcct tggtcacctg atccaattca tggcaggcgt aaaaattcat
    17881 ggcagacttg agatacgcgc gctggatttc cacggcggtc gtcgagcggc cgtccgtcat
    17941 tcttaacgtt cccttcatcg tcaaatcgcg cgagacctgt ttgatcccca tgacaggatc
    18001 gtccagttca atatcaggga catccactcc ggcctcgagc agatccaaga caattcccag
    18061 ggtcccaacc ttgagatacg tggacagctc cgccatgttc gcatcgccga cgatgacgtg
    18121 aagccggcgg tacttgccat agtcggcatg cggctcatcc cgcgagttga cgatcggccg
    18181 cttgaccatg gtattcaggt caaccaggca ttcgaaaaaa tcggcccgct gagatatctg
    18241 aaattcggtg aaactggttt gattttctgc accgacttta ccggcgcccg catagatcaa
    18301 tcgggtgacg aagaagggag ccagcgtctt cacgatccgt tcaaaaggca ccgaccgtga
    18361 aagcatatag ttttcatgat acccgtaact gtttcccttg ccgtctgaat tgttcttgta
    18421 cagcacgaat tggtctcgcc ctctgagccg cgccatgttg tcgaggcatt gaccgagaat
    18481 ccgctcgctg atgcgctcgt atgccacgac ttcacgcggg tttgtgcatt cgggcgtgga
    18541 atattccgga tgagcgccat cgacatagag ccggccgccg ttggcgagga ccttgttcaa
    18601 ttggcggttg taatcggggt tcggccgttc ccgctcgccc tccacttcaa accctcgagc
    18661 atcgagcagc ggattctcgt tctcatagtc ccacaccgca tgaggaaccg aaatgccggg
    18721 ataatgacca atgactgcca gtgaatttga tatcggatcg acggcggaag catcccgtga
    18781 ggcgatgccg aattccgttt cggttcccag tacgcgaggc gtcgtcaggg gtgtggagtc
    18841 ccgcatgggg ggttagagat aatggccggt ggtgatagtt tcgatctgac gggactcggc
    18901 cgggccgccg ctgatcgtcc tgatgtgcac gatcttctca cctttcttac cggcgacttt
    18961 cgcccagtca tccgggttag tcgtattggg cagatcttcg tgctctttga actcttcgcg
    19021 aatcgcccgg atcaggtcgt ccgatcgaag gccgcggccc tcttgggcaa gagcccgttt
    19081 gaccgcatac ttcttggcgc gcgacacgat gccttcaatc aaggcaccgc tggcaaaatc
    19141 cttgaaatac agaatctcct tctcgccgtt ggcgtaagtg acctcgataa atttattttc
    19201 ttcggaggtc gcatacatgg ctcccacagt caggtcggtc aaatgctcga ccaacgcctt
    19261 gggatcgccc ccgtgacgct ttagctcgtc ttcatcgaag ggcagactcg ttcccacgta
    19321 tttcgagaaa atgtccttgg cggcgaccgc atcggggcgc cccaccttca ccttcacgtc
    19381 caagcgtccc gcacgcagca ccgccgggtc gatgagatct tggcgattgc ttgcaccaat
    19441 cacgatgacg ttgcggaggc gctcaacacc gtcgatctcg gagaggaact gtgggacgat
    19501 ggtggattca atatctgagg aaatcccggt tcccctcgtg cggaacaacg catccatctc
    19561 gtcaaaaaaa acgatgaccg ggttcccgtc ggcggcgcgc tctttggcct tcttgaacac
    19621 ctcacgcacc tgccgttcgg actcgccaac atatttattg aggagctccg gtcctttgac
    19681 gtgaaggaaa tagctccgaa cttcctttcc ggtgaggtgc cccagctttt tcgcaatgct
    19741 gttcgccacg gccttggcaa tgagcgtctt gccgcagccc ggagggccat acagcaacac
    19801 gcctttgggt gcactgagct ggtattccgc aaacagatgg ggatgcagga atggcagctc
    19861 gaccgcgtcg cgtacctgct ccagttcgtg ctggagtccg ccaatgttgg cataatcgac
    19921 atccggcact tcctccaaca ccaattcttc ggcttcggac ttcggcagct tttcgatcac
    19981 gcagcccgag cgtggatcga acagaagatg atcgccgaca ctcagccgct cagccagcag
    20041 cggatcgccc aattcggcca ctttttcttc atcgaaatgc agagtgacga gcgcgcggtt
    20101 gccttccagt aaatccttca accgcacgac ttcaccctgg acatcgaatc cgcgagcctc
    20161 gatgacgttc aaggcttcat tcaaaatcac ttcctgtccc ttccgcaaac ccttgcccat
    20221 aagggacggg tgcaagctca ctttcatctt ccgtcccgac acgtacacgt tgcccgtccc
    20281 gtcctggttg agactggaaa agatcgcgta tgtcgaaggc ggagcggtca gtttttcgac
    20341 ttcagcacgc agcgcttcaa tttgcgcttt ggcttcctgg agggtattga ccagtttttc
    20401 gttttgttta ttggcttggt cgagctggta acgggattgg tacagccgac ggatttcttc
    20461 ttccatcgac tgaatttgga cgcgaagctt ttcgatctct ctcgcttgtt cgtcgttacg
    20521 atgaatcacg tctggatctc cctcggacaa ccgcttggtg atgtgcttca cggaatctcg
    20581 gagggaccgt aggcggctgg gtcttccttt cgtatcagcc atggaaccac ctgggacaag
    20641 cgacaagccc tgctaaatcg catcgtccaa gtcttgagcg gattctaaca ctcgttattt
    20701 ttacggtcaa ctagacgact gattccggcg cgcagaatgc gccgatgcgg cacgatcaca
    20761 ccggcgcatt caccgcctcg agcagctcgg tggtcgcggc ttcgacatcg tctgcgcaaa
    20821 ggatggctgt gatcacggcg acgccaaaag cgcctgcacg ccgcaactct cgtgcgcgac
    20881 cggcagtcac gccgccgatt gcaaagatcg gcagtcgaac ggctcgtgca gcctcttcca
    20941 atatcgacaa cccgagcgga ggcccgaaca gtcgtttcga cggtgtatca tacaccggac
    21001 cgagcacgac gtagtccgcc ccgtttgctt cgacttccaa tgcttccgcc actgaatgcg
    21061 ccgacgcccc gagcagtctg agagggggga gcaacctgcg gacgacgccc accggcaggc
    21121 tgttgctgcg cagatggacc ccggcttctt caagcgacag cgccacatcg agacggtcgt
    21181 tgatcaacag ctggccgtta tgaggctcca tcaactgctg tatctcactt gcaagagtga
    21241 ggagatcctt cgcacaaagg tcgcgctctc gcaattgaat cgcaatgttc ccggctctca
    21301 aggcttgggc aaggactgac agtagcggtc tgcccttggt gtgttgacga tccgtgatta
    21361 cgagaagacg cgactcaagc gcagccatga aaggccagaa aagtacgcag gatcattggt
    21421 aggttcgttc aggattgctc aaaacggttt tctgttctca cccgcccacc cgagcgcgcc
    21481 gagacgcgct tgtcaccatg agcaaggccg cagcaagcgc agggacgaag cgccaacctt
    21541 tctcacccgc ccgccccgag ctgccagagc agctctttgc ccgcgactgt acgttgagct
    21601 tcctgagcga tgcgaggacc aagctgacgg gcgttttcag catcctgcta gagcatgcct
    21661 tcgatgggac tgctagccgt cgcgtacagt ttccggggaa tgcggccggc tttgtaagcc
    21721 aaccggccgg catgcacggc gtatttcatg gcctctgcca tcgcaatggg atcttgcgca
    21781 cctgcgatgg ccgtattcat gagtacggca tcggctccgt actccatcgc cagcgccgca
    21841 tctgaagccg tgccgactcc cgcatcaaca acgatgggga ccttgaccgt ttccatgatg
    21901 attttcaaat tatacggatt gcgtatc
//
LOCUS       DASSCQ010000194         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_281926, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000194 DASSCQ010000000
VERSION     DASSCQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281926"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFH05_12525"
     CDS             complement(<1..654)
                     /locus_tag="VFH05_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679092.1"
                     /translation="MATDRSERFLGKVREDTARCRGGERTLQERPLPWVDWRNYWATG
                     DADSKVPGFGALFGNLGGNGRGIRGALLDLEFQRMEMIKFNLFDNSGTYERYAQILRD
                     PAGVFHTPWVEMRVPDRKPPGNDMDDQEYARLVARLKKDGLATHPVDGSQECRGESIR
                     FRDLNGYCNDIYNPLMGSTNQPLARNVQFEAVTFPDLEEQKQSPETRNRHAKRLSLLE
                     "
     gene            complement(895..2418)
                     /locus_tag="VFH05_12530"
     CDS             complement(895..2418)
                     /locus_tag="VFH05_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6679093.1"
                     /translation="MKAEQLASHGSSVLIVEDDPDLSLALDDYLQHEGYTVRSVASGR
                     QALQEAEEHSFGAVVLDLGLPDLSGFAVLRGLEEMDPLLPVIVLTASIQEECTVEALR
                     RGAFAYITKPYNRDELRIILRQAVDVRRLVNKMKLVENALTAKEEQFRQVVQTAPDGI
                     VLADGDGRVVSWNTAAERLFGYSEGEILGQPLTKIMPTRYREDHLRGLERVKAIGGSI
                     QTGRTIEVYGLRKDGSEFPIELSLSTWRSSGQMLSCSIIRDITLRKEAEAQLQLQQIE
                     QQVLLDLIPAMVWYKDSQNRILRVNRHAAESINKTVAELEGQSTYDLYPEEAEKYYQD
                     DLAVMASGQSKLGIVELYEIAAGHKRWVQTDKVPYRDSQGNVIGVLVFAQDITERREA
                     EAALQESERQYRLLMEEASDAIVAVDMDGYFKLANPKACELFGYTREELLGLHVIETY
                     VPAEKEMAQQCLEQLREGKTLSLTRLIQRKDGTAISAEIRGRKMSDDRYFAIIKQVD"
     gene            complement(2547..>3696)
                     /locus_tag="VFH05_12535"
     CDS             complement(2547..>3696)
                     /locus_tag="VFH05_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6679094.1"
                     /translation="SDRALNWTRAQLLEMSVSLPLLCAVTWMVFQSSQAMTGPNYPLA
                     FLTLSILIWVAVRLGPRETVTAILLCAGIAIWGTLRGSGPFARVDHNESLLLLQAFIG
                     VIAVTALVLAVGVAERTRAEQELDELNRSLERRIQDRTSTLQATVEQLQEFNQLKTAF
                     VGIVSHELRTPLTSIKSLSENLFEGVAGPLNEKQKYYASRIQLNTDRLTRMLNELLNL
                     SKIEAGKMDLRPAVLSLQELLIDLVEVFQPLAQRKSITMEIGTMDEMPKVCVDRDKLY
                     EVLANLLENAIKFTPADGRVHIGAQVLDDRHIKMDVTDTGCGIPEEHLPKIFDKFYRV
                     QPGSEPVSGSGLGLAIAKGLIELHGGTIGVESAPGKGTRFYFTLPFVT"
BASE COUNT          772 a         1053 c         1003 g          868 t
ORIGIN      
        1 ttcaagtaac gagagccttt tcgcatggcg gttccttgtt tcggggcttt gtttctgttc
       61 ctcgaggtca ggaaaggtca ccgcttcgaa ttggacgttt ctggcaagcg gctggttcgt
      121 ggagcccatc agggggttgt agatgtcgtt gcagtatccg ttcaggtctc ggaaccggat
      181 cgattccccc cggcattcct gggagccgtc taccggatgg gtcgccagcc cgtccttctt
      241 cagccgggca acgaggcggg catattcctg gtcgtccata tcgttgccag gaggtttccg
      301 gtcgggcact cgcatctcca cccagggggt gtgaaagacg ccggcaggat ctctcaagat
      361 ttgggcgtat cgctcatagg ttccactgtt gtcgaagagg ttgaacttga tcatttccat
      421 ccgctggaat tccagatcga ggagcgcgcc tctgatcccg cgtccgttcc cgccgagatt
      481 gccgaacagc gcgccgaagc ccggcacttt agaatcggcg tcaccggtgg cccaataatt
      541 ccgccagtcg acccatggga gcggtctttc ctgtagggtt ctctcgccgc cgcgacagcg
      601 tgccgtatct tcccgaacct tgccgagaaa tcgctcgctc cggtcggtcg ccacgtctct
      661 tggtccgcgc gtgacgtatg tgaagcagga gctcagctct tttggattaa gggaagcgca
      721 accggaaaca gcaagcagaa gggatgtcag cagcagtgac gtgatcagtc tgtgcattcc
      781 agcgaacgat ttccagtgga tcaatgtcct gtcggtgctc ttctacgctc ttcatcacaa
      841 aatctcaata taagtcttcc tgcaagtggg aggaggcgag ggaatccggt ctgtctagtc
      901 gacttgtttg ataatagcaa agtagcgatc gtcggacatc ttcctgcccc tgatttcggc
      961 agaaatggcc gtgccatctt tgcgctgtat cagtcgtgtc aagctcaagg tcttgccctc
     1021 gcggagttgc tcaaggcact gctgggccat ctctttttct gccgggacat aggtttcaat
     1081 gacatgaagc ccaagtaatt cctctcgagt atacccaaac agctcacacg cctttggatt
     1141 cgccaactta aaatatccgt ccatatctac agccacgatg gcgtccgaag cctcctccat
     1201 taagagtcgg tattgacgct cactctcctg taacgccgct tccgcttccc ggcgttcggt
     1261 aatatcttga gcaaagacca ggacaccgat cacattgccc tgggaatcac ggtagggcac
     1321 cttgtctgtc tggacccagc gtttgtgtcc cgctgctatt tcatagagct cgacaatgcc
     1381 gagtttcgac tggccagagg ccatgacggc aaggtcatcc tgataatatt tttctgcttc
     1441 ctcgggatag aggtcatagg tggattggcc ttcgagctct gccaccgtct tgttgataga
     1501 ttctgcggca tgccgattga cccgtaaaat gcgattctgc gaatctttgt accagaccat
     1561 cgcaggaatg agatccaaca gaacctgctg ctcaatctgc tgcagttgca gttgcgcttc
     1621 agcctccttg cgcagcgtga tgtcgcggat gatactgcag ctcaacatct gcccgctgga
     1681 tctccatgtg ctcagcgaca gctcaatggg aaactcgctt ccatcttttc tgaggccata
     1741 gacctcgatc gttctcccgg tctggattga tccgccgatg gccttgacac gctcaagccc
     1801 tcgcagatga tcttctcggt agcgggtggg catgattttg gtcagcggct gtccgagaat
     1861 ctccccctct gaatagccaa agaggcgttc ggcagcggta ttccacgaga ccactctgcc
     1921 gtcgccatca gccaacacga tcccgtcggg tgccgtctga accacctgtc ggaactgttc
     1981 ctctttcgca gtgagggcat tctccacaag tttcatcttg ttcaccaacc gccgaacgtc
     2041 cactgcttgc cggaggatga tcctgagctc gtcccggtta tacggtttgg tgatataggc
     2101 aaaggccccg cggcggaggg cctcgacggt acactcttcc tggatggacg cggtcaggac
     2161 aatgacggga aggagagggt ccatttcctc caagccgcga aggacggcga agccgctcaa
     2221 gtcgggcaat cccagatcca aaaccactgc tccgaacgag tgctcttcgg cttcctgcag
     2281 cgcctgcctt cccgatgcga cgctgcgcac cgtgtacccc tcatgttgaa ggtaatcgtc
     2341 gagggcgaga gagagatccg gatcgtcctc cacaataagg acggatgaac catgggaagc
     2401 gagttgttca gctttcacgg tctcaccagc tcacgaggtg agggaggtaa tcgacgtcca
     2461 gctttcaaga gcgaaaagga tgggacaaac gcacgcgaaa atcaatagtt ggaagccgaa
     2521 atgttaaggt atacgttcta tttgctctat gtgacgaacg gcagggtgaa gtagaagcgg
     2581 gttccttttc ccggtgcgct ctcgacacca atggttcctc catgcaactc gatgagtcct
     2641 ttcgctatcg ccagccctaa gccggatccc gaaacgggtt ctgaccctgg ttgcacccga
     2701 tagaacttgt cgaagatctt cggcagatgc tcttcaggga ttccgcagcc agtgtccgtc
     2761 acgtccattt tgatgtgccg atcgtccagg acttgtgcac cgatgtggac acgcccatcc
     2821 gctggggtga actttatcgc gttctcgagc aaattggcga gcacttcgta cagtttgtcc
     2881 cggtcaacgc acaccttcgg catctcgtcc atggtgccga tctccatcgt gatcgatttc
     2941 cgctgggcaa gcggctgaaa gacttccacc agatcgatca gcaactcttg gagtgacagt
     3001 accgccgggc gcaggtccat tttccccgct tcaattttgg acaggttcaa cagttcattc
     3061 agcatgcggg tgagccggtc tgtattgagt tggattcgag aggcatagta cttttgcttc
     3121 tcattcaatg gaccggccac tccttcaaat aagttctctg aaaggctctt gatggaagtg
     3181 agcggagtcc gcagttcgtg cgagacgatg ccgacaaagg cggtcttcag ctgattgaat
     3241 tcttgaagct gctcaacggt cgcttgaagc gtactcgtcc ggtcctgaat cctccgttcc
     3301 agcgagcggt tcagctcatc gagctcctgt tccgcacgtg tgcgctcggc aacaccgacc
     3361 gctaacacca aagctgtcac ggcaatgacg cctatgaagg cctgtaataa gagcaagctt
     3421 tcattatggt cgacacgggc aaagggacca gagccccgta acgtccccca gatggctatg
     3481 ccggcacaca acagaatggc ggtgaccgtt tctcgaggcc ctagccggac cgcgacccat
     3541 atcagaatag acagcgtcag aaaggcgagc ggatagttcg gcccggtcat ggcctggctg
     3601 ctctgaaaga ccatccatgt caccgcgcac aggagcggga gcgatacgct catctcgagc
     3661 agttgagcgc gagtccaatt gagcgcacgg tctgaa
//
LOCUS       DASSCQ010000195         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1658587, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000195 DASSCQ010000000
VERSION     DASSCQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658587"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VFH05_12540"
     CDS             <1..1549
                     /locus_tag="VFH05_12540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET6679095.1"
                     /translation="TAFADLVAAHPHDGFLQYRIDQSRLPEVARFLHERLKGRLALLF
                     AIDCRPRAQKYEIQYLFALSSGQPWVLLTVELVGSASLFTSITPSVHAAQWYEREIRD
                     LFGLIPQGHPDLRRLVRHEHWPQGLHPLQKAFPWDKTMGWQQGEYRFRHIEGEGVFEV
                     PVGPIHAGIIEPGHFRFSVAGEPIMQLEVRHFWKHRGIEKLFEQCTLSAALPLAERVS
                     GDTTIGHSLAYCQAVESLLHLEIPRRARFLRSFFLELERLHNHIGDVGAICNDTAYTL
                     AHAHCSRMKEQLMQLNDWLTGSRFLRGTMAIGGVTKDVPAIQQRAVIDSLDRIEKDFA
                     ELESIIFANASLTDRLETTGVLTERTAWDHGAVGVVGRASGIDHDVRRDRPFAAYDEV
                     PVNPVVYRYGDVRARLRVRMDEIHESIRLIREIRQQIPHGSILAAGDHMPQPGEWALS
                     AVESWRGEVLYFVMAGDDGRIHRCKVRDPSFVNWPAIQWAAVGNIIPDFPLINKSFNL
                     SYAGNDL"
     gene            1562..2080
                     /gene="nuoB"
                     /locus_tag="VFH05_12545"
     CDS             1562..2080
                     /gene="nuoB"
                     /locus_tag="VFH05_12545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET6679096.1"
                     /translation="MFRILKKSLKTGLVTGQYPRAQPLVTPTPAAHEKAEPFKSSLTI
                     REVDTGSCNACEMEMNALTNPVYDIERFGVHIAASPRHADALVVTGPVAVNMEHPLKE
                     VYKQTPHPKLVIALGDCAINCGIFKGSYAVTGPVERHIPVDVRIPGCPPRPVEILEAL
                     ASIRSSRTPSDE"
BASE COUNT          507 a          659 c          639 g          498 t
ORIGIN      
        1 gacggccttc gctgacctgg ttgcggcaca tccccacgac ggtttcttac agtatcgcat
       61 cgaccagagc cgacttccgg aggtcgcccg gtttcttcac gagaggctga agggccggct
      121 ggcattgctg tttgccatcg attgtcggcc gcgcgcacag aaatacgaga ttcagtatct
      181 gtttgctttg agctctggtc agccctgggt tttgctcacg gtggagctcg tgggatcggc
      241 ctctctcttt acttcgatca caccctcggt ccacgctgcg caatggtatg agcgggaaat
      301 ccgggatctc ttcggcttga tcccacaagg tcacccagac ctgcgtcgtc tagtgcgcca
      361 cgagcactgg ccccagggac ttcatccact acaaaaagcc ttcccatggg acaagaccat
      421 gggctggcag cagggtgagt atcgctttcg tcacatcgag ggcgagggtg tcttcgaagt
      481 tccagtaggt ccaatccatg ccggcatcat cgaacccgga cacttccgct tctcggtcgc
      541 cggagaaccg atcatgcaac tcgaagtccg ccatttttgg aagcatcgtg ggatcgaaaa
      601 actgtttgag caatgcaccc tgagcgcagc gctccccctc gcagaacgcg tctctggaga
      661 cacgaccata ggacacagcc tcgcctactg ccaggcagta gaatcgctct tgcacctcga
      721 gattcctaga cgtgcccgct tcctgcgctc attctttctg gaattggaac ggcttcacaa
      781 tcatatcgga gacgtcggag cgatctgcaa cgataccgcg tatacgctgg ctcatgctca
      841 ttgcagcagg atgaaagagc aattgatgca gctcaacgac tggttgaccg gatcacgatt
      901 cctccgaggc acgatggcga tcggtggcgt cacgaaagac gtgcccgcca ttcaacaacg
      961 cgcagtcatc gatagcctag atcgcatcga gaaggacttt gcagaactcg aatccatcat
     1021 cttcgcgaat gcctccctca cagaccgttt ggaaacgacc ggcgtgctga cagagcgtac
     1081 ggcgtgggac catggagcgg tcggtgtggt gggtcgggcc tcagggattg atcacgatgt
     1141 ccggagagac cgcccgttcg ccgcctacga tgaagtgccg gtcaaccccg tcgtctaccg
     1201 gtatggagat gtccgtgcac gcttgcgcgt gcgcatggac gagattcatg aatccatcag
     1261 actgattcgc gagatccggc aacaaattcc gcacggctcg atcctcgcag cgggtgatca
     1321 tatgccacag cccggtgaat gggcgctctc agccgttgag agttggcgag gagaagtgct
     1381 ctatttcgtc atggccggag atgacggtcg cattcatcgc tgcaaggtgc gcgacccgtc
     1441 gttcgtcaac tggccggcca tccaatgggc cgccgtcgga aacatcattc ctgactttcc
     1501 gctgattaat aagagcttta acttgtccta cgccgggaat gatttgtagc aggaggagcc
     1561 gatgttccgt attcttaaga agagcctaaa gaccggactg gtgacggggc aatatcctcg
     1621 agcccaacct ttggtaacac caacaccggc agctcatgag aaagccgagc cgttcaagag
     1681 ctccttaacg attcgagaag tggacacagg ctcttgcaat gcctgcgaga tggaaatgaa
     1741 cgctttgacg aacccagtgt acgacatcga gcggttcggg gttcatatcg cggcctcccc
     1801 gcgccacgcc gacgcattgg tcgtcaccgg accagttgcg gtcaatatgg agcacccttt
     1861 gaaagaagtt tataagcaga cgccgcatcc gaaactcgtc atcgccctcg gagactgtgc
     1921 catcaactgc ggaatcttta aaggaagcta tgcggtgacc gggcctgtgg aacgtcacat
     1981 tccagtcgat gtccgtattc ccggctgtcc cccacgacca gttgaaatcc tggaggcatt
     2041 agcgagtatc cggagttctc gaactccgtc tgatgagtga ttctttggtg aaggatgcct
     2101 gagtcatgag ttccagcctg ctatgccgat cgaggcagtg gagggggtct cgcccacctg
     2161 cctcagagaa ccatgtaaaa aagtgacagg ctgaccttga agtatccaca cctgtcactt
     2221 ttgacagtgt cgccgtttgg cccaagttac ataggatgac gaccggcagt tcgtccacct
     2281 cggcatgctt aagaaagaga tca
//
LOCUS       DASSCQ010000196         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_721953, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000196 DASSCQ010000000
VERSION     DASSCQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721953"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFH05_12550"
     CDS             complement(<1..570)
                     /locus_tag="VFH05_12550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6679097.1"
                     /translation="MSAGHAPSLACILISKNEAANIVDCLASVAWAGERIVIDAESSD
                     RTAELARAAGARVIVRPWPGFGAQKNYGMDQVGTEWILILDADERVPPALRDEILGRI
                     GSWKAGEPVAYEIPRRNFFYGAWVRHAGVYPDYQIRLFRRGYARYNDVPVHENLVVQG
                     AIGKLANPLDHFTERRIRDHFRKFGHYTTL"
     gene            complement(567..1589)
                     /locus_tag="VFH05_12555"
     CDS             complement(567..1589)
                     /locus_tag="VFH05_12555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6679098.1"
                     /translation="MLSVIVAVHNQIGHNRLFLKGIRQYTTGAYEVVVIDNHSTDGSA
                     EFFESEGCRVIRNTANLCYPESMNLGTRNSSGAYVCHINNDLYVGPDWNGRLIEAMER
                     FNLDAITPLGLEMMPTPALTDWIQARWATIGQGRLSSGKSEVQLHAKIRAMYGDWEAF
                     CSEVYRSFSEVIFDGLNGSCVMLRRSTLEKIGLLDERVQAADWDLYYTLRKREVELGD
                     VRRCKIFGGCYVHHFIRATVKGRREPFACTHPRLSIHDKWSREEQERLWYKSNYFQPA
                     SGRRSFRDLLRRHVGKPLEKLAREWQRAVSWRWLYVQPEKIVELHRRQIEVLGTGAWQ
                     RGESSP"
     gene            complement(1583..2221)
                     /locus_tag="VFH05_12560"
     CDS             complement(1583..2221)
                     /locus_tag="VFH05_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6679099.1"
                     /translation="MRLSLAIRRWKFRLRRKLQRGPQRRVLDVGSGHRPHEDATHLVD
                     LLPEDNSERGKPIQDLGLPLLVATVESLPFKDKTFDYVYASHVLEHTQDPASACKELM
                     RIARAGYIETPSPFYEQGYNFPHAERGWSFHRWFVYLDEGDTLTFEPKTAKSIDDFCH
                     CRHGQFIRRIYASLKDLNQLQSVLPRQCNYTVLDWKDEFKFAVKPDGTGTAC"
     gene            complement(2288..3058)
                     /locus_tag="VFH05_12565"
     CDS             complement(2288..3058)
                     /locus_tag="VFH05_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6679100.1"
                     /translation="MKRLSWWRRPYFSRRVLEIGPGHNPFAGVTHLLERNVADGRERG
                     GHALLVPAAAHLIVGEATVLPFAAGSFDFVYASHVLEHVDDPEAACREIMRVAAAGYI
                     ETPSPFLEQGLALKDETSPDHWFHRWFVFSPSSGRLVFEPKSPAEVRQFCSCADGQFI
                     REFYESLDFGQAQHFFRRSAKTTVFHWKRPFQVEARDHMVDCRQERRPCRFVGMRRAL
                     IANCNDLLRAGRVRRLPRQFPKSRDVFRKYGQYSLFVH"
     gene            complement(3076..3921)
                     /locus_tag="VFH05_12570"
     CDS             complement(3076..3921)
                     /locus_tag="VFH05_12570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6679101.1"
                     /translation="MKISGFTFCRNAVKYDYPIVESITSALPLCDEFIVNVGRCGDGT
                     LELIQSIRDPKIKIVESVWDESLRQGGLIYSQQTNIALAHCTGDWAFYIQADEVIHED
                     DLPVLRRSMEQHAGNPNVKGLLFRYLHFIADYWSTNPWFYHKAVRVIRNNGEIESCGD
                     AVGFHFKATQQYLQSGPREWLAYSGARMFHYGWVKDPKTMLEKKREQASVYHGANLPP
                     AAAKELTGDAFRFEDYGILKEFRSSHPAVMKARISPTRRWGRRRTRWLNWRFYREVAR
                     RGFRG"
     gene            complement(3940..>4467)
                     /locus_tag="VFH05_12575"
     CDS             complement(3940..>4467)
                     /locus_tag="VFH05_12575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6679102.1"
                     /translation="SLDTDERMTPELKQEIEQVVGQPNASDAYFVPRKNYFLGRWIKH
                     CGWYPDYRQPQLFRKNRLRYREELVHESFDCDGKVGHLSQPALQYPFRDIDHYIAKQD
                     RYSDLMARRMVEQGCRFSAHQLVTHPLAAFVKMYGLRRGFVDGMPGLILSGLYGYYTF
                     MKYAKFWELTKTSER"
BASE COUNT          966 a         1333 c         1215 g          953 t
ORIGIN      
        1 cagggtcgta tagtggccaa actttcgaaa atggtcccgg atgcggcgct cggtgaagtg
       61 gtccaacgga ttggccagtt ttccaatcgc gccttggacc accaggttct catgcacggg
      121 cacgtcgtta tacctcgcgt accctcgccg gaacaggcgg atctggtaat cgggatagac
      181 accggcatga cgcacccatg ctccataaaa gaaattcctg cgtgggattt catacgctac
      241 aggttcccct gctttccagg atcctatccg tcccaggatt tcatcgcgca aagccggcgg
      301 cacccgctca tcagcatcca ggatcagaat ccattcggtc ccgacttgat ccatgccgta
      361 gtttttttgc gcgccaaaac ccggccacgg ccgcacgatc acccttgcgc cggcggcacg
      421 cgccagttcg gctgtgcggt cgctgctttc cgcatctatc acgatccgct cgccggccca
      481 cgcgaccgat gcgaggcagt ctacgatatt cgccgcttcg ttcttcgaga tcagaataca
      541 ggccaaggat ggggcatgcc ctgcgctcac ggggatgact cgcccctctg ccaggctccg
      601 gtcccgagca cctcaatttg gcggcggtgc aactcgacga tcttttcagg ttgcacatac
      661 aaccagcgcc aggacacggc ccgctgccat tccctggcca atttctcgag cggcttgccg
      721 acgtgtcgac gcaggaggtc ccgaaacgaa cgccggccgg aagccggctg aaaataattg
      781 gacttgtacc acaaccgttc ctgctcttct ctcgaccatt tgtcatggat cgacaaccgc
      841 gggtgcgtac aagcaaacgg ctctcgtctt cctttgacgg tcgctcggat gaagtgatgg
      901 acgtagcagc ctccgaaaat cttgcagcgt cggacatccc cgagctccac ttcacgtttt
      961 cgaagtgtgt aatacaagtc ccaatccgcc gcctgaactc gttcatccaa caatccgatt
     1021 ttctcgaggg tagaacgccg aagcatgacg caggaaccgt tgagcccatc gaagatgacc
     1081 tccgagaaac tccgatagac ttcgctgcag aacgcttccc agtccccgta catcgcacga
     1141 atcttcgcgt ggagttggac ttcgctcttc cccgatgaga gacggccttg cccgatcgtc
     1201 gcccatcggg cctggatcca atccgtcagc gccggcgtcg gcatcatttc caagcctaac
     1261 ggcgtgatgg catcaagatt gaagcgctcc attgcctcga tcagccgtcc gttccaatcc
     1321 ggtccgacgt agagatcatt gttgatatgg cacacatagg ctccgctcga gttccgtgtc
     1381 ccgagattca tggattcggg atagcacaga ttcgccgtgt ttcgaatgac gcggcatcct
     1441 tccgattcga aaaactcagc cgaaccgtca gtggaatgat tgtcgatgac caccacttcg
     1501 taggcaccgg tcgtgtattg gcgaatgcct ttgagaaaca gtcgattgtg cccgatctga
     1561 ttgtggacgg cgacgatcac gctcaacatg ccgtacctgt tccgtccggt ttcacggcga
     1621 atttaaactc atccttccaa tccaacaccg tatagttaca ttgccggggg agcacgctct
     1681 gcagctggtt gagatccttc agggaagcgt atattcgcct gatgaattgt ccatgccggc
     1741 aatggcaaaa atcgtcaatc gacttggccg tcttgggttc gaatgtgagc gtgtcccctt
     1801 cgtccaaata gacgaaccac cggtgaaacg accagccgcg ctccgcatga gggaaattgt
     1861 acccctgctc gtagaacgga cttggcgttt cgatgtagcc ggcccgtgca atgcgcatga
     1921 gttctttaca cgcggaagca gggtcctggg tatgctccaa cacgtgggag gcatagacgt
     1981 agtcaaacgt cttgtctttg aacggcaggg attcaaccgt ggcgaccagg aggggaaggc
     2041 ccagatcttg aatcggtttc ccgcgctctg aattgtcttc aggaaggagg tcaaccaagt
     2101 gggtagcatc ctcatgcggg cgatgaccgg atccgacgtc caagacccgc cgttgaggcc
     2161 ctcgttgcaa cttccgcctg aggcgaaact tccaacgacg tatggccagg gacaatctca
     2221 ttgacttgct ctccttcaga cgcgcgtggc gtacgggacg ttgttcggcg cgacgaatac
     2281 gtcgaggtca atggacaaag agcgagtact gtccatactt gcgaaagaca tccctcgact
     2341 tgggaaactg ccgtgggaga cggcgaactc tacccgcccg cagcagatcg ttgcaattgg
     2401 cgattagcgc ccgcctcatc ccgacgaagc ggcatggcct tcgttcttgc cggcaatcaa
     2461 ccatgtgatc gcgcgcctcc acctgaaaag gcctcttcca gtggaacacg gtggtcttcg
     2521 ccgaccgtcg aaaaaaatgc tgggcttgac cgaagtccaa cgactcataa aattcccgga
     2581 tgaattgtcc gtccgcgcag gaacagaatt gtctgacctc cgccggcgac ttgggctcaa
     2641 agacgaggcg gccggacgac ggagaaaaca cgaaccaccg gtgaaaccaa tggtccggag
     2701 atgtctcgtc tttcagggcc agcccctgct cgaggaacgg ggaaggggtc tcgatgtagc
     2761 cggccgcagc aacccgcatg atttcccggc aagccgcttc gggatcgtct acatgctcca
     2821 ggacatgcga cgcgtagacg aagtcgaatg atccggcggc aaaagggagg accgtagctt
     2881 ccccgacaat gaggtgggca gcggccggca cgagcaaggc atgtccgccg cgttcccggc
     2941 cgtccgcaac attcctttcc agtaaatgcg tcacgccggc aaagggattg tgccccgggc
     3001 cgatctccaa cacgcgacgc gagaaatatg gccgtctcca ccaagacaat cgcttcatga
     3061 cggtcggatc ggtcgctacc cgcgaaaccc tctgcgggcc acttcgcggt agaaccgcca
     3121 gttcagccag cgggttcgcc tgcgacccca ccgacgtgtt ggagagatcc gcgctttcat
     3181 aacggcggga tggctgctgc gaaattcctt caaaatgccg tagtcctcga atcggaaggc
     3241 gtcccccgtt aattctttgg cagcggcggg aggaagattg gcgccgtgat agaccgacgc
     3301 ctgttcccgc tttttctcga gcatggtttt cggatccttc acccatccat aatggaacat
     3361 ccgtgcgcct gaataggcga gccactccct cggaccgctt tgcagatatt gctgagttgc
     3421 cttgaaatga aatccgacag catcgccgca cgactcaatc tcgccgttgt tccggatcac
     3481 ccggacggcc ttgtgataga accagggatt cgtcgaccag taatccgcga tgaagtgcag
     3541 gtaccggaac agcagcccct ttacgttggg attcccggca tgctgctcca tcgatcgccg
     3601 caggaccggc aggtcgtctt catgaatcac ttcatcggcc tggatgtaga aggcccaatc
     3661 gcccgtacag tgggcaagtg cgatattggt ctgctgcgaa tagatcaggc cgccttgtcg
     3721 gagcgattca tcccagactg actcgacgat tttgatcttt ggatctcgga tcgactggat
     3781 gagctccaag gttccatccc cgcaccggcc aacgttgaca atgaactcat cgcacagagg
     3841 cagcgccgac gtgattgatt cgacgattgg ataatcatac ttcacggcat tgcggcagaa
     3901 ggtgaatcca ctgatcttca tgacaggcga cgggtgcgtc tacctttcgc ttgttttggt
     3961 cagctcccag aactttgcgt acttcatgaa cgtgtaatag ccatagagcc cggaaagaat
     4021 caaccccggc ataccgtcga caaagccccg tcgcagtcca tacatcttga cgaaggccgc
     4081 cagaggatgc gtcaccagct gatgtgctga aaatcggcac ccctgttcga ccatgcgccg
     4141 cgccatgaga tccgaatacc ggtcctgctt ggcgatgtaa tggtcaatgt cccgaaaggg
     4201 gtattgcagc gctggttgtg atagatggcc gaccttgccg tcacagtcaa agctttcatg
     4261 cacgagctct tcacggtagc gcaagcggtt cttgcggaag agttgcggct gccgataatc
     4321 cggataccag ccgcagtgct tgatccagcg tcccaggaag taattctttc tggggacaaa
     4381 ataggcatcg gacgcgttcg gttgtccgac cacctgttct atttcctgct ttaattcagg
     4441 agtcatccgc tcatcggtat cgagact
//
LOCUS       DASSCQ010000197         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1867802, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000197 DASSCQ010000000
VERSION     DASSCQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867802"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="hemC"
                     /locus_tag="VFH05_12580"
     CDS             complement(<1..563)
                     /gene="hemC"
                     /locus_tag="VFH05_12580"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET6679103.1"
                     /translation="MTGVIHERSTLVLGTRGSKLAVHQSEWVQARIRELAPHLTVTLR
                     RIQTSGDKILDVPLAKIGGKGLFVKEIEEALLSGDIDFAVHSMKDVPTQLPAGLDLLC
                     VPVREDPRDALVSRDGRRFKDLPSGARIGTSSLRRQAQLLQARPDVSISVLRGNLDTR
                     LKKLREGHFDAIVLAAAGLRRLGWERD"
     gene            complement(565..909)
                     /locus_tag="VFH05_12585"
     CDS             complement(565..909)
                     /locus_tag="VFH05_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679104.1"
                     /translation="MGVSRTGPKLFTCPQCRKTYLGHDPVPDCPSCGYDYRAKGGFRW
                     DVLVYLLSVLGMLSYFLVSSSYRSLITESVTTSPGSASPHSTSAPASGLEKLPGADRS
                     PFVYPPERGADR"
     gene            complement(927..2264)
                     /gene="hemA"
                     /locus_tag="VFH05_12590"
     CDS             complement(927..2264)
                     /gene="hemA"
                     /locus_tag="VFH05_12590"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET6679105.1"
                     /translation="MHIVVVGLSHKTAPVEIREKLAVPESRLGEALTRLCAYQGIKEG
                     ILLSTCNRVEVYAVVEDLEAGYSRIQEFLADAHLSVASEQLTPHLYWQTGDRAITHLF
                     RVACSLDSMVVGESQILGQVKDAFETALTHKTTGLILNKVVKKAISVAKRVRTDTRIA
                     ETAVSVSYAAVELAKKIFSDLRDKTVLLVGAGEMAKLAARHFMAAGVRHVLVTTRTPQ
                     HAVVLADRFNGTAVPFEQFREEMAAADIVLVSTGAAHYLVGSDDVQRAVKQRMNRPMF
                     LIDISVPRNIDPAVRHVDNAFLFDIDDLKFRVEQNRGERLQEAERAEHMVIEEVAAVR
                     QWLQTLEVTPTIVALRGRVEDIKRAEVEKALARLGHLPPQERALVEAMASSIVNKLIH
                     NTMVTLKSEVTTADGAAFVEAARRFFNLGEAGPIRDEIEIDESTTRTSVNKPG"
     gene            complement(2266..3069)
                     /gene="ccsA"
                     /locus_tag="VFH05_12595"
     CDS             complement(2266..3069)
                     /gene="ccsA"
                     /locus_tag="VFH05_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET6679106.1"
                     /translation="MAAVFFMVTMALYFAATVSFLALLLRRADALSTFALTITAVGFA
                     THTVALVMRMLTGPGASTPSIHEALSFFSWMLILVFLAVEFRHRLHVLGSFIVPLALV
                     SLVSAAALPETVPTLQPVFRTLWVHVTLSMLGTVGFAVAFVAGVMYLIQDGLLKSKRF
                     NVLYSKLPALDFLDDLNQQSIVLGFPLLTLGIITGAISAEFARGTYVNWNPEQTWALV
                     TWLFYFCVLVGRLTVGWRAKRAAYLTIIGFAGVVLTLVGVALKSFGSVS"
     gene            3131..3907
                     /locus_tag="VFH05_12600"
     CDS             3131..3907
                     /locus_tag="VFH05_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6679107.1"
                     /translation="MARQKSQQTSRKASNSPERRRRSGTLFIVGTPIGSLDDLTIRAR
                     AVLRQVSIVAAENPLATQALFSKHRISNTITSYGRRDEHKIAILLDRLNAGHDIALVS
                     DCGMPVIYDPGRLLITAARNAGYPVTVVPGASAVTAAAALSGYSGDRLIFLGRLPRTR
                     PQLDQLCSTLKRESATTIMFVHPPVLPHILNSIRRVLPNRAVTLAVNMTKKDERLCQG
                     SATSLLEIARSLSRDAELTLVLAGARERPGTTQLRRINTR"
BASE COUNT          877 a         1191 c         1113 g          759 t
ORIGIN      
        1 atgtcccttt cccatcccaa gcggcgcaga ccggcggccg ccagcacaat ggcatcgaaa
       61 tgcccttccc gcagcttctt caatcgggta tcaaggtttc cgcgcaacac gctgatagag
      121 acatcgggcc gcgcttggag caattgcgct tgacggcgaa ggctgctcgt gccgatcctc
      181 gcgccggatg gcagatcttt aaatcgtctg ccgtcgcgac tgaccagggc gtctcgcgga
      241 tcctctcgta cgggcacgca gagcaagtcc aaccccgccg gtaattgggt cggcacatct
      301 ttcatgctgt ggacggcgaa atcaatgtcg ccggacaaga gcgcctcctc aatttctttg
      361 acgaagaggc ctttgccgcc gattttagcc agcgggacgt cgaggatttt gtcgccggac
      421 gtttggatgc gccgcagcgt gaccgtaagg tgcggagcga gctcccggat tcgggcctgc
      481 acccactcgc tctgatggac cgccaacttg ctgccgcgtg tcccgagaac caatgtggag
      541 cgctcatgaa tcacgcccgt catgtcagcg gtccgcgccg cgttccggcg ggtagacaaa
      601 cggcgaccgg tctgctcctg gaagcttttc tagacccgat gcaggcgcag acgtggaatg
      661 tggcgaggcg gatccggggc ttgtggtcac ggattcagta atcagactgc gataagagga
      721 cgagacaaga aaataactga gcatccccag caccgacagc agatagacga gcacatccca
      781 ccgaaatccg cccttcgcac gatagtcgta gccgcaggag gggcaatcgg gtaccggatc
      841 atgccccaga taagttttcc ggcactgcgg acacgtaaaa agtttcggac ccgtcctcga
      901 cactcccatg aatgttcctt gcgccatcaa ccgggtttgt tgacgctcgt gcgagtcgtg
      961 gactcgtcga tctcaatctc gtcacgtatg ggcccggcct ctcccaggtt gaaaaaccgc
     1021 cgggccgctt cgacaaacgc cgcgccatct gcggtggtca cttccgactt cagcgtcacc
     1081 atcgtgttat ggattaactt gttcacgatc gaggaggcca tggcctccac cagcgcccgc
     1141 tcttgtggtg gaagatgccc caggcgagcg agcgccttct cgacctcggc ccgcttgatg
     1201 tcttcgacac gacccctcag cgcgacgatc gtgggtgtga cctccaacgt ttgcaaccat
     1261 tgccggactg ccgcgacttc ttcgatcacc atgtgctcgg cccgttcggc ctcttggagg
     1321 cgctctccgc gattttgctc gaccctgaat tttaagtcat cgatgtcgaa gagaaatgcg
     1381 ttgtcgacgt gtcggaccgc cggatcgatg ttacggggaa cggaaatgtc gatcaagaac
     1441 atcggccggt tcatgcgttg cttgacggct cgttgaacgt cgtcgctgcc cacgagataa
     1501 tgtgcagcgc ccgtcgagac gagcacgatg tcggccgcgg ccatttcctc ccggaactgc
     1561 tcgaacggca cggcggtccc gttgaaccgg tcggccaaga cgacggcatg ctgcggcgtt
     1621 ctcgtggtca cgagcacatg ccgcacaccg gcggccataa aatgccgcgc cgccagcttc
     1681 gccatttcgc cggcaccgac gagcaacaca gtcttgtccc gcagatcgga aaaaatcttt
     1741 ttcgccaact cgacggccgc atagctgacc gagacggccg tctcggcaat tctcgtatct
     1801 gttcgcaccc gtttcgcgac cgagatcgcc ttcttgacca ctttgttgag aatcaggccc
     1861 gtggttttgt gggtgagcgc ggtctcaaat gcgtctttga cctgacccaa aatctgagat
     1921 tcaccgacca ccatggagtc cagactgcag gcgacgcgaa ataaatgcgt gatggcccga
     1981 tcgccggttt gccagtagag atgaggcgtc aattgctccg atgcgaccga caggtgggca
     2041 tcagccagga attcctggat gcggctgtac cccgcttcaa gatcctcgac gacggcatag
     2101 acctcaaccc gattgcaggt cgacaagagg atgccttcct tgattccttg atacgcacac
     2161 aatcgagtca gggcttctcc gaggcggctt tcgggaacag ccagtttttc ccggatttcc
     2221 acgggtgcgg ttttatggct gagtccgacc acgacgatat gcatatcagg agactgaccc
     2281 gaaacttttg agggccacac ccaccagcgt gagcaccact ccggcgaatc caataatcgt
     2341 caaataggcg gcgcgtttgg cacgccatcc gaccgtgagc cgcccgacaa gtacgcaaaa
     2401 gtaaaacagc caggtcacca acgcccaggt ctgttccgga ttccaattca cgtaggtgcc
     2461 gcgtgcaaat tcggctgaga ttgcgccggt aatgatccca agcgtgagaa gcggaaaacc
     2521 caacacaatc gactgttggt tcaaatcatc aagaaaatcc agggcgggca gcttcgaata
     2581 cagcacgttg aagcgtttgg acttgagaag gccatcctga atcagataca tgactcccgc
     2641 gacaaaggcg acggcaaagc cgactgtgcc gagcatgctg agcgtcacat gaacccacag
     2701 cgtacgaaac accggctgaa gggtgggaac cgtttcggga agcgcagcgg ccgacaccag
     2761 agagacgagc gccagcggca cgatgaacga tcccagcaca tgaaggcggt gacgaaattc
     2821 cacggcgaga aacacgagga tcaacatcca ggagaaaaac gacagcgctt cgtgaatgct
     2881 cggtgtcgag gcccccggcc ccgtcagcat ccgcataaca agcgcgacgg tgtgtgtggc
     2941 aaaaccaacg gcagtgatcg tgagtgcgaa ggttgagagg gcatcagccc gccgcagcag
     3001 cagggcaagg aaggacaccg tggcagcaaa atacagtgcc atagtgacca tgaaaaacac
     3061 ggcggccatg aagtctatcc cctgaacttt tcaggacaat tggcctgggc gagtgcgtag
     3121 aattatatcg atggcgcgtc aaaagagtca acaaacctct cggaaggcgt ccaattctcc
     3181 ggagagacgt cgtcgctcag gcacgctctt tatcgtggga acgcctatcg gatcgctgga
     3241 cgatctcact atccgcgcgc gagcggtcct cagacaggtt tccatcgtgg cggcagaaaa
     3301 cccgctggcc actcaagcgc tgttttcaaa acatcgaatc agcaacacca ttaccagcta
     3361 tggccgcaga gacgaacaca agattgccat cctgctggac cggctcaacg caggccacga
     3421 tatcgcactc gtctctgact gcgggatgcc ggtcatctat gatccagggc gactcttgat
     3481 taccgctgcc cgcaatgccg gctatcccgt cacggtcgtg ccgggcgctt cggcagtgac
     3541 cgcagccgcc gccttgtctg gatactcagg ggaccggctc atcttcctcg gtcgcttacc
     3601 acggacacgt ccccagctgg atcaattgtg ttcgacactc aaacgagaat cagcaaccac
     3661 aatcatgttt gtgcatccac ctgttcttcc gcatatcctc aacagcatca ggcgcgtcct
     3721 tccaaaccga gccgtcacac tggcggtcaa catgacgaaa aaggatgaac ggctgtgcca
     3781 gggatccgcg acatcgctgc ttgagatcgc tcggtctctc tcgcgagacg cagagctcac
     3841 gctggtgctc gcaggcgcgc gggaaagacc ggggacgact cagctgcgtc ggattaatac
     3901 gcgataggat tgttggagag gctcctggga caagacctcg
//
LOCUS       DASSCQ010000198         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1505536, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000198 DASSCQ010000000
VERSION     DASSCQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505536"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFH05_12605"
     CDS             complement(<1..870)
                     /locus_tag="VFH05_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET6679108.1"
                     /translation="MRESRLSTITLGQSQPRSSLAPCLVSLFLVAAPVFAGTPEGEQD
                     RGAWRTAAPAAMKRTEVAAATVGGKIYLVGGFEEPSISNVVNLSITPVVEEYDASTDR
                     WSSKAPMPVGLHHVGVGVVDGRLYVIGGYKQSGLSVWGPVATLYSYDPAADAWTELPA
                     MPTARGALSVTAHEGKLYAIGGFDGKGNSAAVEVYDPVRNAWSTRASLPTPRDHLATA
                     TLAGKVYAIGGRLKGDYHRNLSVTEVYDPGSDKWTKAPDLPTARSGITAAEIGGRVYV
                     FGGEGANGTFHENE"
     gene            complement(913..2046)
                     /gene="ydiK"
                     /locus_tag="VFH05_12610"
     CDS             complement(913..2046)
                     /gene="ydiK"
                     /locus_tag="VFH05_12610"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HET6679109.1"
                     /translation="MKNLEIQPPETDPTESDRPINAPTTPHDLARSTLSVLVMVGLIA
                     ASFWILRPFIPAMLWATMIVVAGWPLMLQLEALLWGRRGLAVTVMTLALLLVFVVPFS
                     LAVTALVTHLDEIVDWAQSLKSLTLPEPPSWVESIPLAGTKIASSWRDFASYGPDELA
                     RSAAPYARVTGSWLLAQLGSLGAMTGQFLVTVLISAVLFARGDSAVTDLRRFGRRLAG
                     ARGEQAVNLAGQAIRGVALGIVLVAMGQAVLGGIGLFVAGVPFAAVLTALMFLFGIVQ
                     IGVVPVLAIAVGWLYWQGSHGWATGLLAWTIFVGTIDNVIRPILIQKGARLPLVLIIA
                     GVIGGLVAFGVVGIFVGPVVLGVGYMLFEAWIQEEPLDSEEGV"
     gene            complement(2033..2182)
                     /locus_tag="VFH05_12615"
     CDS             complement(2033..2182)
                     /locus_tag="VFH05_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679110.1"
                     /translation="MKAGYRLILVDHDGVMVSEFQLTERDLADPPAFVAAIKQSIEQV
                     EDEEP"
     gene            complement(2412..2654)
                     /locus_tag="VFH05_12620"
     CDS             complement(2412..2654)
                     /locus_tag="VFH05_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679111.1"
                     /translation="MDIQRAVKAFIAEGKDLYHRLRSSGEGLSDLDLVALREQLYILD
                     TEAGHLQELKDSRSDGAEFIFSNRPSVVQPFSRKAA"
     gene            2944..3249
                     /locus_tag="VFH05_12625"
     CDS             2944..3249
                     /locus_tag="VFH05_12625"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET6679112.1"
                     /translation="MQTYVSLVKFTQHGLQTMKDKGIERAEAVKHNAQSLGGKLIQAY
                     YCLGEYDVIAIWEFPDNKTAMKAAVLNASLGHIEITTMPAVDREEWKSLLQQTIGKK"
BASE COUNT          820 a         1139 c          979 g          668 t
ORIGIN      
        1 ttcgttttca tggaaggttc cgttcgcgcc ttcgccgccg aacacataga cacgccctcc
       61 gatttcagcg gcagtgatcc cgctcctggc cgttggaagg tccggcgcct tagtccactt
      121 gtccgagccg ggatcgtaca cttccgtgac ggaaagattg cgatggtagt cgcctttcaa
      181 ccgcccgccg atggcgtaga ccttcccggc caacgtcgca gtcgccagat gatcacgcgg
      241 agtgggcagc gacgcgcgcg tgctccaagc gtttctcact ggatcgtaca cttcgactgc
      301 tgcgctgttg ccctttccat caaagccacc gatcgcatac agtttgcctt catgcgccgt
      361 gaccgacaag gccccccggg cggtcggcat cgcggggagc tccgtccagg cgtccgcggc
      421 cggatcgtag gaatacaggg tggcgactgg tccccagacg ctgaggccgg actgtttata
      481 gccgccgatc acgtacaggc gcccgtcgac tacgccgaca cccacatgat gcagaccgac
      541 cggcatgggc gccttcgagc tccatctgtc ggtagaggcg tcgtactctt cgacaacggg
      601 tgtaatcgag agattcacca cattgctgat gctcggctct tcgaagccgc ccacgagata
      661 gatcttgcct ccgaccgttg cggcagccac ttccgtccgc ttcatggccg ccggcgctgc
      721 agtgcgccag gcaccacgat cctgttcccc ctccggcgtt ccagcaaaaa ccggtgctgc
      781 gacgagaaac agagatacta ggcagggtgc caacgatgac cgaggttgtg actggccaag
      841 tgtgattgtc gatagccgac tctctcgcat ggcttgcctc atcaatctca atctcctacc
      901 gaagaaccac atctacacgc cttcctcaga atcgagcggt tcttcctgta tccaggcttc
      961 aaacagcata taccccactc ccagcacgac cggtcccacg aaaattccca ccacgccgaa
     1021 tgcgaccaga ccgccaatga cgccggcgat gatgagcacc agcggtaacc gcgcaccctt
     1081 ctgaatgaga atcggccgta tcacgttgtc gattgttccg acaaagatag tccaggccag
     1141 gagcccagtg gcccagccat ggctcccctg ccaatagagc cagccgacgg caattgcgag
     1201 caccggcaca acgccaatct gcacgatgcc gaacagaaac atcaacgcgg tcaacaccgc
     1261 tgcgaacggc acgcccgcca caaacagacc gattcccccc aacaccgctt gccccatggc
     1321 cacgagaaca atgccgagcg cgacgccgcg aatcgcctgc ccggccagat tcactgcctg
     1381 ttcgccgcgt gctcctgcga ggcgtcgccc gaaccggcgc agatccgtca cggcgctgtc
     1441 tcctcgggca aagagcacgg ccgaaatcag gactgtgacc agaaactgcc cagtcatggc
     1501 gcccagacta ccgagttgag ccagcagcca gcttccggtc accctggcat acggcgccgc
     1561 ggatcgggcg agctcatcag ggccgtacga ggcgaaatcg cgccatgaag atgcgatctt
     1621 cgtaccggcg agagggatgc tttcgaccca ggatggcggc tcaggcagag tgagggattt
     1681 gagcgactgc gcccagtcaa cgatctcgtc gagatgggtc acgagcgctg tcactgccag
     1741 cgaaaaggga acgacgaata cgagcaacag cgccaacgtc atgaccgtca cggccaagcc
     1801 gcgacgtccc cagagcaacg cttcgagctg caacattaat ggccagcccg cgacgacgat
     1861 cattgtcgcc cagagcatcg caggaataaa tggccgcaga atccaaaacg aggcggcgat
     1921 gaggccgacc ataaccagaa cggagagagt agaccgcgcg aggtcatggg gtgtggtcgg
     1981 cgcatttatc ggccggtcgc tctccgtggg atcggtttcg ggaggctgta tctcaaggtt
     2041 cttcatcttc aacctgctcg atcgactgtt taatcgcggc cacaaacgca gggggatcgg
     2101 ccagatcacg ctccgttagc tggaattccg acaccatcac gccatcgtga tcgacaagga
     2161 tcaaacgata accagctttc acagtcacac ctctcaatag gctggcctga ggaacgtgat
     2221 cctcccccca atatctactc attgtcaaac gggtacttca gatgccggtt caacgactga
     2281 tgctcggcag gcggttctca gagtcaggca caccgacgac cccgaaattg tataggcgcc
     2341 tccccggcgg aaagggccgc cgggctgagg caccgtcgga tctcatgacc gattcagaag
     2401 gctatgttca actaggccgc tttgcggctg aacggctgga caacagatgg cctgttgctg
     2461 aagatgaact cggctccgtc cgatcgggaa tccttaagct cctgcaaatg accagcctcc
     2521 gtatcaagaa tgtataactg ctctcggagc gcgaccagat ccagatcgga gagtccctca
     2581 ccactggagc ggagtcgatg gtacaaatcc ttgccttctg cgatgaatgc cttgacggcg
     2641 cgttgaatat ccatggtacg tgcctccatg ttttacatga tgcgcaacgc ctggtcgatt
     2701 ctactatgtg ctgctttttg cagagggcgg cctagtaaaa ccccttgtct gcgtgagcgg
     2761 atgcctccag gaacgcacct catataataa aatggtccta accctgctct ttcttctcgc
     2821 tccgggtgat ggaggccatg cgttcacctc tcgcttgagc agcggggtct atcatccatc
     2881 ccctacattc ccctacaatt tgttcgaaca tcgcacttca cagctcaccc gggaggaccc
     2941 actatgcaga cctacgtcag cctcgtgaag ttcacgcaac acggtctcca aacgatgaag
     3001 gacaagggga tcgagcgagc cgaagccgtc aagcacaatg cccaatccct gggcggaaag
     3061 ctcatccagg cttactactg cctcggcgaa tacgacgtca tcgccatctg ggaatttccg
     3121 gacaacaaga cggccatgaa agccgcggtc ctcaatgcct cgctcgggca cattgaaatc
     3181 acgaccatgc cggccgtcga ccgcgaggaa tggaagagtc tgctgcagca gacgatcggc
     3241 aaaaaataag acggatgatg aggagctgtt ggtctccttt gctcgcccaa cgcgcacacc
     3301 ggaacaatcg tatttttaga cagacggtgc tcgttgaagc gcgcagtgag agaccaatca
     3361 gccctcatcc taggagaaac acacaagcgc gccttgaagg agccttcaag cctgcaccca
     3421 tcgcaggtcg cgtgaggact accatccacc tcgccaggga agcgcacacg aaaccagcct
     3481 ggtcacgaga aaacgaacca gcaagcttgt ctgaccatta atggggaagc tcactgagga
     3541 aattatgcgg tgtcgtccga gtaagagact cagccggaac atcacggtgg gccttgcagc
     3601 tgtgct
//
LOCUS       DASSCQ010000199        34120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_416106, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000199 DASSCQ010000000
VERSION     DASSCQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 34120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34120
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416106"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..895
                     /locus_tag="VFH05_12630"
     CDS             176..895
                     /locus_tag="VFH05_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679113.1"
                     /translation="MRVLTVGGLKIRLAGGMDGSGSGKGPAVILVHGYGAPGDDLVVL
                     AEVLGGPTGTRWIFPEGPLPLSMGYGDSRAWWVIDMARLEADRAAGRVRDLSGEVPRG
                     LPQARHAFETFLTALPQVLPVDYRQTVIGGFSQGAMLTCDIVMRTSFPVAGLIQLSGS
                     LLAKQDWRPTVGKRAGLRVFQSHGTQDPILPYVMAERLRDELVKAGLSVEWQSFRGGH
                     EIPQSVIKRLGEFLVQVLANS"
     gene            934..1680
                     /locus_tag="VFH05_12635"
     CDS             934..1680
                     /locus_tag="VFH05_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6679114.1"
                     /translation="MIRGDRLDGTDRHIVFITGLLSTRWGNKSRALRQWCEEQGWSFC
                     CYDVRGFGDSEGDFTDYTLSDWLADARAVLSMLRNDAAARSIGGGFTLVGNSLGGWIA
                     WLIAQECEEVDRLILIAPAFNMMGVRAQTISPERRHEWHSAGWMPWDDDPAHRDWPLS
                     WKWVVESEAYWQSNFDRLRHVRTTIVHGLGDTVIHPEGSRAFVEDLRRHDLSFPVELL
                     LVAGDHRLSSPDHMEQLRRLLLDGTISPLC"
     gene            1674..1844
                     /locus_tag="VFH05_12640"
     CDS             1674..1844
                     /locus_tag="VFH05_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679115.1"
                     /translation="MLTLVHKECGGPALEPNTSVGEVCAVPIDRFPFACFTCLEEIID
                     ESEVRLSEELGI"
     gene            1930..3612
                     /gene="ettA"
                     /locus_tag="VFH05_12645"
     CDS             1930..3612
                     /gene="ettA"
                     /locus_tag="VFH05_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET6679116.1"
                     /translation="MATNDKQVIFSLVNVGKVYPPKKQVLREIYLGFYYGAKIGVLGL
                     NGSGKSSLLRIIAGIDQNFTGEISRSKGYSIGLLEQEPQLDPAKTVKEVVEEGKHELV
                     ALLKEYEAVSNKIGDSSPDEMEKLLDKQAQLQEKIEAANGWELEHHLEIAMDALRCPP
                     AEQKVGTLSGGEKRRVALCRLMIQEPDILLLDEPTNHLDAESVQWLEQHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGHGIPFQGNYSSWLEQKQERLAQEEKAESKRKKTLEH
                     ELEWIRMSPKARQSKGKARLNRYEELVSQREDLRTDDLEIYIPPGPRLGDVVVQAKGV
                     TKGFGDKLLYEDISFDLPKGGIVGVIGPNGAGKTTLFKMIIGKDKPDAGSIKIGETVK
                     LGYVDQDRSLDGNKTVYEVISEGQDTIKLGKVEVNARGYCARFNFAGTDQQKKVKDLS
                     GGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRALEEGLENFAGCAVISSHDRWFL
                     DRIATHILAFEGDSKVVWFEGNYSDYETDRKKRLGKEADQPHRIRYRKLTRS"
     gene            3638..4525
                     /locus_tag="VFH05_12650"
     CDS             3638..4525
                     /locus_tag="VFH05_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6679117.1"
                     /translation="MPSERKPLVWITGAGGLIGRYLLQSAPRWASDYRAQGLTRAELD
                     LTNPSALAAAFERQQPDIVIHCAALSRTKDCERDPDAARRINVDATAQLADLASDRPF
                     LFVSSAEVFDGGKGWYDEADAPAPINVYGATKLAAERIILQNVGHTVVRIVLTAGTSL
                     LRDRSFVEDMCRAARAGSFMTLYADEFRCPLPAGVIARAIWELICGGRTGLYHLGGRE
                     RLSRWEIGQLLLEWFPELQGRLVKGSSRDHTGAPRPADLSLSTTKIQHVLSFPIPGMQ
                     SWLGERRGRGDDLWDYPVP"
     gene            4522..5328
                     /locus_tag="VFH05_12655"
     CDS             4522..5328
                     /locus_tag="VFH05_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HET6679118.1"
                     /translation="MNSDPLFLVMVGYGAMVVFMLVLWGVQVVHRNASIADVGFCAGL
                     IVVVLWYARYGFGDPERKLFVAAMVCVYAGRLGLFLLFNRVIGKAEDARYQEMRRSWG
                     ASEQAKMFGYFQLQAAAVAAFSLPFLVLIQNPRPAFGFVELAGFFIWIAAISGEAAAD
                     RQLARFRSRPWNRDRVCRDGLWYYSRHPNYFFEWLHWWSYVVMAIGAPGWPLTLIGPA
                     GMGWALLRVTGVPLAERQALKTRGADYRRYQETTNAFVPWLPKRLPVKSD"
     gene            complement(5315..5527)
                     /locus_tag="VFH05_12660"
     CDS             complement(5315..5527)
                     /locus_tag="VFH05_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679119.1"
                     /translation="MTQLLTALFLVFVMACTGDKAKDLLETAEFEERQMNIPHAKQLY
                     EEVIRLYPSSQEAEKAKARLAKLSQT"
     gene            complement(5579..7165)
                     /locus_tag="VFH05_12665"
     CDS             complement(5579..7165)
                     /locus_tag="VFH05_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET6679120.1"
                     /translation="MNSILGTAVPSSFSLLIGGLRHDGLASVVVFLVALPLCIGITIA
                     SGAPTGSGIVTGIVAGLVVGWLSGCPLQVSGPAAGLTVIVYELIREQGLEAFAVIVIA
                     AGFIQILWSWLQLGQWFRAVSPAVIHGMLAGIGALIFASQFHIMIDDLPKGSGLENIL
                     SLPLAVWKGLIDDDSTPMNHHLAARIGVLTIGTMVLWNVVLPKRFHVMPAVLLGVASA
                     TAVSALLDIPIQRIEVQDNLLSIVHFPSIQMLSHFFDTSILAEALGLAFIASVETLLS
                     ASAVDQLHTGPRTRYNRELLAQGVGNMICGFLGSLPMTGVIVRSSANVEAGARTRASA
                     ILHGVWLLLFVSLLPSVLRFIPTASLGAVLVYTGYKLMNFGILPELKRHGRGEVVIFF
                     VTMGTIVFVNLLTGVVLGLVLALAKLLHTTQNLEAFYAHNTESGKMTLHLQGIATFVS
                     LPRLAATLEIAPPHAEIHLDFASLRHIDHACLNLLQNWQQQHEAHHGRVVVDWDKMRG
                     ASYARRQTLRKTSWWQHWLE"
     gene            complement(7250..7555)
                     /locus_tag="VFH05_12670"
     CDS             complement(7250..7555)
                     /locus_tag="VFH05_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679121.1"
                     /translation="MLPVPQTARQRMIELLSGTRLSAYQLAQMLGISERQVEDHLVHV
                     VKTIARDRKRRFILEPSTCPECHFIFQDRRKLTRPSRCPRCRSESITAPRYGIDGSS"
     gene            7631..9076
                     /locus_tag="VFH05_12675"
     CDS             7631..9076
                     /locus_tag="VFH05_12675"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET6679122.1"
                     /translation="MTLSDRDEMAELALKRVRESGAEYGDIRLMQSMSQTISGEDRRI
                     ASVRDVADSGFGVRVLYHGGWGFAASSILSLEEVPRVADLAIEIAKGSASLASERARL
                     VDEPIHRDRVVTANRIDPFTVPLDQKTMLLQDVMEYVQRQPEVVRSHASLWARGDKKL
                     FVSTEGTRLEFDLLAVQGEFTATARHDGRFASRSFTTPHLRTGYELIESANFMAEAPR
                     IASQAVEKVKAPAVRPGTYDLVLDPEHLSLTMHESCGHPSELDRALGYEANYAGTSFL
                     TPEKRGSFQYGSSQVNLVADNTEPDTLAATGYDDDGVACQKWDIVREGVFVGYCTNRE
                     VASKIGEARSKGSNRADSWGSVPIVRIANIGLEPGQASLQDLLADVKRGIYIEGHGSY
                     SIDQRRYNFQFGGDAFWLIENGKRTHMLRDVIYHGITPEFWNSCDGVADRRYRRRYGF
                     ITCGKGQPGQSGWMTHAASHARFRRAAVIKG"
     gene            9084..10427
                     /locus_tag="VFH05_12680"
     CDS             9084..10427
                     /locus_tag="VFH05_12680"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET6679123.1"
                     /translation="MSITTVKPWPKMTGRDEFRFLADLVLKRSSADQTVVNLYDQHSG
                     TTRFASSRIIQNVDTRRGTLTVTVAFDGHRGSASTTDFSAGSVQDTLARAERIARVSP
                     EDPEYLPPPDPQIFPTLQTSRPETAAAGPPRRLEYANEAIGQCRIENLEAAGIVSSSV
                     ASVGIAASNGLFAQEERTEARFSLTVQAEDATGWSAAAHRSIDHLRIQDRTLTAINKA
                     KRSRDVQELPPGRYWVILEPAAVAGLWSWMLWTLDAKSYEKGTSPFADQLGRQILDER
                     LTLQNLPGHPDLLGTGFTHEGLPSEDAVWIEKGVLKQLAYDRFTAKQQRIKRIATLEA
                     PCLSASGPTALKTQELIKQCERAILVTNFWYIRTVNPTDLTLTGMTRDGTFLVEDGQI
                     TSAVRNFRFHESPLRVFRHVDACTEPAEAVTSETGKMLVPAVILRDFHFSSVTRF"
     gene            10438..12081
                     /locus_tag="VFH05_12685"
     CDS             10438..12081
                     /locus_tag="VFH05_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679124.1"
                     /translation="MTSDREFEAGWLTRLAAALLGLLMSSPPLYAETPLYDNLGSLHH
                     PITTTSALAQRYFDQGLRLVYAFNHEEAIRAFEEAAKQDPNAPMPYWGVGLALGPNIN
                     AAMSKEDERRAIEAVKQARNRLAAASPAERAYIEALTKRYVATRRAARASHDKAYADA
                     MRTVWRAYPDDPDAGVLFAEALMDLRPWDFWTAEGKPKPGTEEIVSTLEEVIKKHPDH
                     PGACHYYIHAVEASGKPERALACADRLPGLMPGAGHLVHMPAHIYARVGKYHESAVRN
                     AEAAQVDEHYLTSAHLTGDYADGYYSHNLHFLWSSLMMEGRSAEALQAAASLVSTITE
                     DEARSNRSKEPYLTTKLFALIRFGRWEELLREPPPSKGLRLVEGIWRLGRGLALVGTG
                     RFPGAEGERQALMTLTKQFRRSKNQQDKLERDLLRIAERLLAGQIAAQRQRYDEAIRA
                     LKEAVALDDSLQGAEPPHWPLPVRQYLGPVLLLSSRAAEAEAEYRADLKWYPENGWSL
                     FGLLQSLQAQRKESEAQEIAGRFEKAWTHADVTLAASRF"
     gene            12210..12743
                     /locus_tag="VFH05_12690"
     CDS             12210..12743
                     /locus_tag="VFH05_12690"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679125.1"
                     /translation="MRYVVGVMGPAKARRKDVENARVLGELIARREWVLLTGGRDVGV
                     MHAASQGAKKVARSLTVGVLPTTRDKISKYVDVAIVTEMGSARNNICVFSSQVIVACG
                     LGGSGTVSEVALALKAGKEVVLLGATPVEETFFKNLGGRLVSSVQTPEDAINVILRRY
                     ERSDGDAQHPAQSWGHF"
     gene            12841..13464
                     /gene="msrA"
                     /locus_tag="VFH05_12695"
     CDS             12841..13464
                     /gene="msrA"
                     /locus_tag="VFH05_12695"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6679126.1"
                     /translation="MTALTWAMPILLVLGIVAGEQSTVHEGQAAESGTMAKAYFAGGC
                     FWCMEEAFEKVDGVLDVLSGYMGGSVKNPSYEDVSAGRTGHAESVEVRYDPRKLTYPQ
                     LLEVFWRNVDPVTPEAQFCDHGNQYRAAIFYQGEEEKRVAEESKRAIEQSKRFSTPIA
                     TQVTAASEFYPAEEYHQDFYKKNPFRYKYYKFTCGRAQRLEALWGKS"
     gene            13610..14215
                     /locus_tag="VFH05_12700"
     CDS             13610..14215
                     /locus_tag="VFH05_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6679127.1"
                     /translation="MLTWLGGMSPCHTERLRRIGQASCTVLLAMLIVVVVPTATNSDF
                     DSHQHYSPQEIRRAIVFYARRYRLDPALLRAVMKAESGYRQHVVSRKGAIGLMQLTPD
                     TAATLRVANVYDSMENIRGGAKQLRHLLNLYGSDLHLALAAYNAGVHRVKGRTVPRIR
                     ETRAYVRKVLKYYRALKGEQRWVGPVHPGPKSRGGVNPPVK"
     gene            14534..15694
                     /locus_tag="VFH05_12705"
     CDS             14534..15694
                     /locus_tag="VFH05_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6679128.1"
                     /translation="MTKRLTNQRMAELGQSEIRAMTQACVAAGGLNMAQGVCDTPVPP
                     VVLKSAAEAMVQGRNSYSRFDGLPELRQALAVKLDRDNGIRADPETDITVSAGATGAF
                     HSACMALLNPGDEVILFQPYYQYHISALLAVEGRPVIVPTTGPDWTFDPEAVERAITP
                     STRAILVNSPGNPSGKVFSREELEVVARIATEHDLFVFTDEIYEYFVYDGRRHVSMAS
                     LRGMADRTITIGGYSKTFSITGWRIGYSVAASRWAQAIGAMNDLLYVCAPTPLQAGVA
                     AGIQELPDAFYRELAKEYQHKRDRFCAALAGAGLPPSVPQGAYYVLADAIRLPGRTGK
                     DRAMHVLKHAGIAGVPGEAFFAGLDGSRYIRFSYAKTDADLEEACRRIAGLP"
     gene            15736..16218
                     /locus_tag="VFH05_12710"
     CDS             15736..16218
                     /locus_tag="VFH05_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679129.1"
                     /translation="MKMYMIPSSWKIALVSLPLVLSVACTSPPVMHEEIGQPSLPSTS
                     GIPQDRRVLAGEWDYEDGAVVTLTLDEQGNGSYPWKDGRFVTHSLSDHVWQGMWIQKE
                     NDREGGFTVQFSPDFSDGEGQWWYTRIEDDRAPTQKGGTFRLTRKNSSSAANETSPAP
                     "
     gene            16224..16538
                     /locus_tag="VFH05_12715"
     CDS             16224..16538
                     /locus_tag="VFH05_12715"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6679130.1"
                     /translation="MHERKHVRFAVQRPVSFRGDLLSGEGTILNISRQGCAILSDTAL
                     ETEAYLQLHLQLLEQEQPVHVHLAAVRWCTARKFGVEFIKMHHDVGERLKQFVELLES
                     NA"
     gene            complement(16545..18305)
                     /locus_tag="VFH05_12720"
     CDS             complement(16545..18305)
                     /locus_tag="VFH05_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET6679131.1"
                     /translation="MIVQRNVNKTIDPRAFDLLIDESFIRQGDLIKAIQDSLQQGIEL
                     EDLLIQQYRVPKAAIGKALSKFFECPYIPYDERMVVDPQLIKDLSHDYLWKHVWVPLK
                     ANGSVLDVLIDDPQDIVRGHDIQRAFPGMTIRYAVALKGDIKRFLQSTIGMTDTGSIE
                     DTLGELVHEARAEESLAGDVDAIDENDSAIVRLANQIIVEAYRQEASDIHIEPYSDRK
                     ETAIRLRVDGTCSTYMRIPAAYRRALVSRIKIMSNLDIAERRKPQDGKLRFRLNRDRE
                     IEVRVATMPTAGNNEDIVMRLLTAKEPMPLEAMEFAPSTLAGLLEVIDKPHGILLCVG
                     PTGSGKTTTLHAVLHRLNTDARKIWTAEDPIEITQEGLRQVQVLPKIGFTFAAALRSF
                     LRADPDVIMIGEMRDKETAEVAVEASLTGHLVLSTLHTNSAVETVTRMLDLGCDPFNF
                     ADAMQGVLAKRLCKRICGHCKELYHPTVQEYDKLVREYGSNSWDTLAVPPYGDAFMLS
                     RGRGCDHCNHTGFKGRVALHELLRGSDELRNLIQNRAKTAEMISLAKKAGMITLIQDG
                     ILKVIAGLTTYDQVRTVAMK"
     gene            complement(18494..19399)
                     /locus_tag="VFH05_12725"
     CDS             complement(18494..19399)
                     /locus_tag="VFH05_12725"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6679132.1"
                     /translation="MLPSLKIALLGTGLLGKAIAERLTAVGHRVVVFNRTRTKALPLE
                     AQGIAVVGSAEIAVTQADLVLLLLTDAPAIHSVLFSPACSAALSGKTIVQMGTIDSNE
                     SLEIEREVKRRRGTYCEAPVLGSLAEAKAGTLLVMVGGTEDQFAALAPLFRSLGREPR
                     FVGAVGKAAALKLALNQLIAAETLAFGLSLGLIRRAGISVDTFMTILRESALYAPTFG
                     KKLPRLLKRDYHHPNFSTRHLLKDVRLFVREAGARGLTTRPLEGMASLLEAAIAQGLG
                     EADYSALYDLLDPPPSGSPPSPHNG"
     gene            complement(19399..20199)
                     /locus_tag="VFH05_12730"
     CDS             complement(19399..20199)
                     /locus_tag="VFH05_12730"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET6679133.1"
                     /translation="MESELAQLSSTIRKAGARAKDLAHKGFEIHTKKDRSPVTTADLE
                     VNRILREMQEMHFPDDGWLSEESPDDPARLDKARVWIVDPIDGTKAFVNKLPEYCISV
                     GLIQGGVPVLGAIFNPSTDELFTAIRGQGVRLNGSPHTPETPGDAMSHILVSPWEYRA
                     GRWAGLDNQMRFRPMFSIANALAVVAAGRVQATLTIEPENEWDLAAGVLLVQESGGSV
                     ADAEGRPFTFNQPIPRFRGVIAVAATAPTDLHPLLQTHADRARTKKKR"
     gene            20370..21425
                     /gene="corA"
                     /locus_tag="VFH05_12735"
     CDS             20370..21425
                     /gene="corA"
                     /locus_tag="VFH05_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET6679134.1"
                     /translation="MKLVQQRSKKTGLPPGTLVHIGERRTAKVTFTVFHYGAAGCEEL
                     QPHQADQLAPPADESVVWINVSGVHNVEVLEAIGKQFVLHPLLLEDIANTDQRPKLDD
                     YETYLFLVIKMMSLTDRKEIAVEQVSLVLGRNYVLSFQENGTDVFQPVRERLRGGKGR
                     LRQSGADYLLYALVDAIVDQYFAVLEFMGEKIELLQQTVVADPKPETLNEIHGLKRQL
                     LFVRRAVWPLRDVMNNLSRSDCPFLQEATKLFFRDVYDHVVQIVDTIETLREMVSASL
                     DIYLSSVSYRLNAVMRVLTVITTIFMPLSFIASIYGMNFEHMPELKSTWGYPIVLVAM
                     TVVGLGMLILFRNKRWL"
     gene            complement(21549..21905)
                     /locus_tag="VFH05_12740"
     CDS             complement(21549..21905)
                     /locus_tag="VFH05_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679135.1"
                     /translation="MWLFRPRFFLVLLFLAAGALLVAFNYSYSDGNRAGYVQKFSKKG
                     WFCKTHEGELAMTTVPGTAPVLWEFTVWDDAVADQLSEAMGKRVVLHYKEYRYLPTTC
                     FGDTPYFVDRVEVQND"
     gene            complement(21947..22570)
                     /gene="leuD"
                     /locus_tag="VFH05_12745"
     CDS             complement(21947..22570)
                     /gene="leuD"
                     /locus_tag="VFH05_12745"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET6679136.1"
                     /translation="MQAFTELTGLVAPLDRVNVDTDQIIPKQFLKTIKRTGLREGLFF
                     DWRKQKDGSPDPLFFLNQPRYQGATILLTRDNFGCGSSREHAPWALLDQGFRCIIATS
                     FADIFYNNCFQNGILPVVLQADAVESMMKDVLAMPQYRATIDLGQQTVTTPHGTVYGF
                     SIDPFRKDCLYRGLDSIGLTLQHEPAIAAYEARRRAEAPWLFSDFPS"
     gene            complement(22606..24009)
                     /gene="leuC"
                     /locus_tag="VFH05_12750"
     CDS             complement(22606..24009)
                     /gene="leuC"
                     /locus_tag="VFH05_12750"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET6679137.1"
                     /translation="MAGQTLFDKIWESHVVRAERDGTTLLYIDRQLVHEVTSPQAFEG
                     LKLAGRRPRRPGATLAVPDHNVPTTDRRLGIADQVSALQIQTLEDNCRDFGIRLFNMS
                     DIRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLI
                     QKRPKTMEVRVDGVLSDRCSAKDVILAIIGEIGTAGGTGYVIEYTGSVIRGFSMEGRM
                     TLCNMSIEAGARAGMVAPDEHTFNYIKGRPLAPTGAAWDQAVRAWQHLQTDDDATYDA
                     TVVVKAEDIAPQVSWGTSPGMVTGVDGKVPDPKTIVDEKTRQATERALAYMGLKANMP
                     ITDIRIDRVFIGSCTNSRMEDLRLAASLAKGKHIAKTVRAMVVPGSGLIKQQAEAEGL
                     DRIFTAAGFEWREPGCSMCLAMNADVLEPGERCASTSNRNFEGRQGAGGRTHLVSPAM
                     AVAAAVEGHFVDIRRWS"
     gene            24142..24594
                     /locus_tag="VFH05_12755"
     CDS             24142..24594
                     /locus_tag="VFH05_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HET6679138.1"
                     /translation="MNQTIVQWTQRLLDSYRHWMGTELLERTGDPELQAHALFESPVV
                     VVSHGMQTDPVLNYGNRMALKLWEMTWEQLIETPSRLTAEPVNRAEREWMLEQARVRG
                     YLDTYRGVRISRSGRRFLVEGAVIWNVLDAHRQQIGQAATFAHWTWLS"
     gene            complement(24610..24840)
                     /locus_tag="VFH05_12760"
     CDS             complement(24610..24840)
                     /locus_tag="VFH05_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HET6679139.1"
                     /translation="MITPGRYRHYKGHDYEVIGVARHSETEESFVVYRALYGERGLWV
                     RPAAMFLDTVTVDGRSVLRFTRLPVQPSEEDG"
     gene            24881..25576
                     /locus_tag="VFH05_12765"
     CDS             24881..25576
                     /locus_tag="VFH05_12765"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6679140.1"
                     /translation="MGKQTASLTFALLAATGLAAMVPSCQQQTWESVMTAGQEAVRQG
                     NYAEAEHMFQVAVQKAETFGREDRRVAVSLSQLAQVYAGQGKHAEAEPVYLQALKIYQ
                     AVHGENHADVAATLNNLGVLHRMYGQYAEAEPLLSRSLAIKERLLGPNHPDVALSVAN
                     LGQLYVAQGQPAKAEPLYRRALSIKREALGMSHPEVAKTLEDLANVLRKLGRSPEAVA
                     LDLQAREIRAKRS"
     gene            25609..25851
                     /locus_tag="VFH05_12770"
     CDS             25609..25851
                     /locus_tag="VFH05_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679141.1"
                     /translation="MPNANQPLSPNWRMSAMVARFVGIIAIALGFMLGMYGLDEQGSR
                     WLHTALGLLVTGMLAQGYALYCSIKRMQQLHDRPKT"
     gene            25848..26279
                     /locus_tag="VFH05_12775"
     CDS             25848..26279
                     /locus_tag="VFH05_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679142.1"
                     /translation="MKRAGRTVPSLSISLLLVFATLAACGGPWRDGYFKKGVNRLTQD
                     EISEKLGPPHTAKTPALGGDTIWTYRVPYSDKDLDPMDLSGITNAANEAASLIGKGAQ
                     DGPKPMLYCYRYVLTFNEQKVLKRWKREECVPGTRDTLHAG"
     gene            26304..27122
                     /locus_tag="VFH05_12780"
     CDS             26304..27122
                     /locus_tag="VFH05_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679143.1"
                     /translation="MITWLPQVDSSIVMDSLKSLLLLLSVLIARTLIVRSISRNAALT
                     MEAKRRWVVSVRNSMVFVFLLGLVVIWAHELQAFAVSLVALAAALVLATKELLLCWSG
                     AALRVGGKVYMVGDRIQIAGHRGVVLDHDIFATKLLEIGPGQASHLYTGRVTVFPNSL
                     LFTNALVKENPNQEYGLYTLTVPLAAGEDWKQAEARLLEAGRAECASFMAEASRQMKL
                     LEQQNLLEAPSPEPRITIQLSDVGRIVLVLRFPAPDRGRSRIEQAILRRFLGEA"
     gene            27243..27611
                     /locus_tag="VFH05_12785"
     CDS             27243..27611
                     /locus_tag="VFH05_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679144.1"
                     /translation="MRTMLTVAVVVLWLMNVTVPAVADDAITGEMNQLQADIKAEKEA
                     MKADTKTKKAEMKQLKKEHKKNIKAKKREMREKNKANKNRMKAQKDAMKTQGQDLNAV
                     GQDMKPAVPDLPAPQAPGAQ"
     gene            27714..28490
                     /gene="xth"
                     /locus_tag="VFH05_12790"
     CDS             27714..28490
                     /gene="xth"
                     /locus_tag="VFH05_12790"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET6679145.1"
                     /translation="MKIATYNVNSLRRRLPVVLDWLEKEKPDVLCLQETKVQDSEFPL
                     MALLPSGYEITYRGMKSYNGVAVLTRTKPESVRAGLDDGGEPDEPRLLHVVVNGIAII
                     NTYIPQGFEIGSPKYAYKLEWLKRLRAYFEKHLSPSLPAIWCGDMNVAPRAMDVHSPE
                     KHLKHVCYHETAREAYASTVAWGFYDVFVRLYPERQQFTFWDYRAPSSLDANKGWRID
                     HMLATASLADRCLRADVDVEPRRARDASDHTFLWAEFSLP"
     gene            complement(28934..29104)
                     /locus_tag="VFH05_12795"
     CDS             complement(28934..29104)
                     /locus_tag="VFH05_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679146.1"
                     /translation="MTCQKCKGLMIAESRPDYSTDGTVLRCINCGLVLDPLIEQNRLA
                     RTRSTHAAMHAA"
     gene            29336..29695
                     /locus_tag="VFH05_12800"
     CDS             29336..29695
                     /locus_tag="VFH05_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET6679147.1"
                     /translation="MGFLHLMTACALLIGSHVMAAAGGSVAMDRTEVARGKQIFAKYC
                     AGCHGSQGKGDGYQLLGPSPANLISPTTEERSDDDLLRTIHEGKPNMPAWKHRLSRQD
                     SRDVLTYIRSLSAVQRK"
     gene            complement(29780..30664)
                     /locus_tag="VFH05_12805"
     CDS             complement(29780..30664)
                     /locus_tag="VFH05_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6679148.1"
                     /translation="MKIVAGVDWSDAAFAAVEQLGLLYQPTDITLVHGVDLGLFEYPI
                     VAEAANLQGYDIFRKSMFEAGCESLDRAATMLPNDIPKPRQLCEFRKPARFLLDTATA
                     MAADLIVVGTHDHSRVAELVLTSVSHRVLLHAMQPTLLVKGDAKPIRRVLVAIEGRDD
                     GRQICAWLSRHPFRTSVEATVLTAVPMLPTPMPELSPIPPFESWSEELMAQAQASVRA
                     CAEALLPSRYSASSRVMKGEPAPVISETAKEFDLVVVGSHGREGLDRFLLGSVSHAIV
                     HQVTCPVLVVRSGQPTPS"
     gene            complement(30695..31366)
                     /locus_tag="VFH05_12810"
     CDS             complement(30695..31366)
                     /locus_tag="VFH05_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET6679149.1"
                     /translation="MVVVFRDRAEAGRLLAEKLESYRGDPSGLILALPRGGVAVGYEL
                     SVSLRLALDVFVTRKLSTPDNPEYAIGALSETGALYLNPEAVEAFRLSHADLETIIDN
                     ERQELARRQQLYRHGLPLAQIRDKTVILVDDGIATGATFLATIEGVRKLSPGRLIAAI
                     PVAPLDSLPSVRNRVDELIVIATPEPFMAVGHHYHNFTQVDDAQVLAYLNAARQSESA
                     QTASG"
     gene            complement(31566..32072)
                     /locus_tag="VFH05_12815"
     CDS             complement(31566..32072)
                     /locus_tag="VFH05_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679150.1"
                     /translation="MPTITLTKPFERHPSSVQNKTEAGLRWRVLIVDPHSSIREMIRM
                     ILDGYADLIEVAGEAADADEAVEQAKAMAVDLVLMDAHLANGVEATRQIRQILPQVVI
                     LGMSAEYTPYLYNAMIAAGAVAFVRMEDAADHLFRSIVFAMCTYGPMHRHVHPAAPSV
                     RHNAASFA"
     gene            32413..32679
                     /locus_tag="VFH05_12820"
     CDS             32413..32679
                     /locus_tag="VFH05_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679151.1"
                     /translation="MKISHVWKSGSAKKKTKPTYKIRWELLGLSRPDQMQSSLSMDAI
                     RSEVSLLATQGLSERTQSRSTGPAVSGRGHKRSITGSRRRGAEK"
     gene            33177..>34120
                     /locus_tag="VFH05_12825"
     CDS             33177..>34120
                     /locus_tag="VFH05_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6679152.1"
                     /translation="MLPGHWRPGWNEHWADNKPVPDTAGSWKLTELEVTDGRFLALAI
                     SGGGSRAANFGAAVMLELEHRKLLEQVDVISGVSGGTLPAAYYGLGEQAGAFTESALR
                     KALGFDFQGSWIRRWFLPHNIFRYWLSDFTRSDIMVQVFNNQLYHDATFRHLLPHPKI
                     LLNSTVHNDHTRFTFTDERFAKLHSVLATYPVANAVNASSAFPGAFQDVTLQWYMEPP
                     QYMHLYDGGPIDNLGVQAITEYINRNILGTSLDRLFPNGCLIVIIDATPASEHPELNA
                     GKSSRSLIDYFVDTNALDAMDALLLESRRSLLAGMGIP"
BASE COUNT         7191 a         9743 c        10253 g         6933 t
ORIGIN      
        1 ggcattggcc ggcatcgatc acgatatccg cacgaaacag gtccaattca tcatcaacga
       61 agtggaaggg aacgagcagg gtgcgttcgt tcctctctat caacaacccg tcatcgagtc
      121 ctccctctac ggtctcgtgg cactgcttca atacttatac gttcggtaat cattcatgcg
      181 agtgctcacg gttggcggac tgaagattcg gctggcgggc ggtatggatg gcagcggcag
      241 cggcaaggga cccgccgtca tcctcgtcca tgggtatggc gcgcccggag acgatctcgt
      301 cgtgctggcc gaggtcctgg gcgggccgac cggaacccgc tggatttttc ccgaggggcc
      361 cttgccgctc agcatggggt acggagattc gcgcgcctgg tgggtgatcg atatggcacg
      421 tctcgaagcg gatcgcgccg ccgggcgcgt gcgcgatttg tcgggtgagg tcccgcgtgg
      481 gcttccgcag gcacgccacg ccttcgagac gtttctgacg gccttgcctc aggtgctgcc
      541 cgtggattac aggcagaccg tcatcggagg gttctcgcag ggtgccatgc tcacctgcga
      601 catcgtcatg cgcacgtctt ttcccgtcgc ggggctgatc cagctctcgg gctctctcct
      661 tgccaagcag gactggcgac cgaccgttgg aaaacgggct ggcctccgcg tgttccaaag
      721 ccatggcaca caggacccga ttcttcccta cgtgatggcc gaaaggctgc gggacgaact
      781 ggtcaaagca ggcctgtcgg tggaatggca gagtttccgc gggggtcatg agatccccca
      841 gtcggtcatt aagcggttgg gcgaattctt agtccaagtt ctggccaact catgacgctc
      901 aggcgtttcg agctcaaagg ttcagacggg aagatgatac gcggggatcg cttggacgga
      961 acggaccgcc acattgtctt catcaccggc ttgttgtcga cacgctgggg gaacaagagc
     1021 cgggccttaa ggcaatggtg cgaggagcag ggatggagct tttgctgtta cgatgtccgg
     1081 ggattcgggg attccgaggg ggactttact gattacacgc tgtccgattg gctcgcggat
     1141 gcgcgagccg ttctctcgat gctccggaac gacgcggctg cccgcagcat cggtggcggg
     1201 ttcaccctcg tcggcaactc actcggcggc tggatcgcat ggttgatagc ccaagaatgc
     1261 gaagaggtgg acagattgat cctcattgcg ccggccttca acatgatggg agtgcgagcg
     1321 caaaccatct ctcccgaacg gcgccatgag tggcacagtg ccggctggat gccctgggac
     1381 gatgatccgg cgcatcgtga ttggccgcta tcctggaaat gggtggtgga gagcgaagcc
     1441 tactggcaat cgaacttcga ccggctcagg cacgtgcgga caaccattgt ccacgggctt
     1501 ggagatacgg tgattcaccc ggaaggcagc cgagcgttcg tggaggatct gcgccgtcac
     1561 gatctgtcgt ttccggttga acttctgttg gtcgctggcg atcatcgact cagcagccct
     1621 gaccatatgg agcagctgcg tcggctcctg cttgacggaa cgatctcacc cctatgctga
     1681 cgcttgtgca caaagaatgc ggcggcccgg ctctcgagcc gaacacatcc gtcggggagg
     1741 tctgtgccgt gccgatcgat cgcttcccgt ttgcctgctt tacctgtctc gaagaaatta
     1801 tcgatgaatc cgaggtgcgg ctctccgaag agctgggcat ctaggaggat tgtaggagcc
     1861 ctccggcctc tggtaaggtg cctcttttgc gaaggactcg cgaacagaag aaggagcagc
     1921 gaccaactca tggccacgaa cgacaagcaa gtcattttct ctctcgtcaa tgtcggcaaa
     1981 gtgtatccgc cgaagaagca agtgctgcgt gagatctatc tgggcttcta ttacggcgcc
     2041 aagatcggcg tgctgggttt gaacggatcg gggaagagca gtttgttgag gatcattgct
     2101 ggtatcgatc aaaacttcac cggcgaaatc tcacgatcga aagggtacag catcggtttg
     2161 ctcgaacagg agccgcagct tgatcccgct aagacggtca aggaggtcgt tgaggagggc
     2221 aagcacgagc tggttgcgct cctgaaagaa tacgaagccg tcagcaacaa gatcggcgat
     2281 tcatccccgg acgaaatgga gaagctgctg gataaacagg ctcagctgca ggaaaagatc
     2341 gaggcagcaa acggctggga gctggaacat catctcgaga tcgccatgga tgcgcttcgt
     2401 tgtccccccg ccgagcaaaa ggtcggcacc ctgtccggag gagaaaagcg gcgtgtggca
     2461 ctctgccggt tgatgatcca ggaaccggat atcctgctgc tcgacgagcc caccaaccat
     2521 ctggatgccg agtccgttca atggctggaa cagcacctcc agcaatacaa gggaaccgtc
     2581 atcgcggtga cgcacgatcg ctatttcctc gacaatgtgg cgggctggat tttagagctc
     2641 gaccgcggcc acggcattcc atttcaaggc aattactcct cgtggttgga gcaaaagcag
     2701 gaacggctgg cccaggagga aaaggcggaa tcgaaacgca aaaagacgct cgagcacgag
     2761 ttggaatgga tcagaatgtc gccgaaggcg cggcaatcga agggcaaagc ccgcctcaat
     2821 cgctacgaag aattagtcag tcaaagggaa gatctgcgca ccgatgattt ggagatttac
     2881 atcccgccgg gcccgcggct gggcgatgtg gtcgtacaag ccaaaggcgt gaccaaaggc
     2941 tttggcgaca aactgctcta cgaagatatc agcttcgacc tgcctaaggg cggcatcgtc
     3001 ggcgtcattg gccctaacgg cgctggcaaa acgacgctct tcaagatgat catcgggaag
     3061 gacaaaccgg atgccggctc aatcaagatc ggcgaaacgg tcaagctggg gtacgtcgac
     3121 caggaccgca gccttgacgg caacaaaacg gtctacgaag tgatttcgga agggcaagac
     3181 accatcaagc tggggaaggt ggaagtcaac gcgcgcgggt actgtgcgcg gttcaacttt
     3241 gcggggaccg atcagcagaa gaaggtcaag gacctgtcgg gtggtgagcg caaccgtgtg
     3301 catctcgcgc ggatgctcaa ggaaggcgcc aacctcatca tcctggacga gccgacaaac
     3361 gatctcgacg tgaacacctt gcgcgcgctc gaggaagggc tcgagaattt tgcgggctgt
     3421 gcggttatca gcagccacga ccggtggttt ctcgaccgga ttgcgacgca catcctggcg
     3481 ttcgaaggcg acagcaaggt ggtctggttc gaaggcaact acagcgacta cgagaccgac
     3541 aggaaaaaac gcctcggcaa ggaagccgac cagccacatc gcattcgtta tcggaagctc
     3601 actcgcagct agcaggccta atagagaacc ccccactatg ccttccgagc ggaagccgct
     3661 cgtctggatc accggcgcag gaggcttgat cggccggtat ctgttgcagt cggcccctcg
     3721 gtgggcttct gattaccggg cccagggtct gacccgggcc gaactcgatc tcacgaatcc
     3781 gtcggccctt gcagccgcgt tcgagcggca acaaccggat atcgtcatac attgtgcggc
     3841 gttgagccgg acgaaagact gcgagcggga tcccgacgcc gccagacgca tcaatgtcga
     3901 cgcgacggca cagttggcag acctggccag cgaccggccc tttctgttcg tgtccagcgc
     3961 ggaagtcttt gacggaggca agggatggta tgacgaagcc gatgcaccgg ctccgatcaa
     4021 tgtgtatggt gccacgaagc ttgccgccga gcgcatcatt ttgcagaatg tcggacacac
     4081 cgtcgttcgg atcgtgctga ccgcgggcac ctcattgctg cgtgaccgca gttttgtgga
     4141 agacatgtgc cgggcggcca gggccggcag tttcatgacg ctgtatgcgg atgagtttcg
     4201 ctgtccgcta ccggcagggg ttattgcccg tgcgatctgg gaactgatct gtggcggaag
     4261 aacaggcctc tatcatctcg gtggccgcga gcgtctctcg cgctgggaaa tcggccagct
     4321 gcttctcgag tggttcccgg agctgcaggg ccggttggtc aaagggtctt cacgtgatca
     4381 cacgggcgct cctcgaccgg ccgatctctc attatccacc acaaagatcc agcatgttct
     4441 gtcttttccg attccaggca tgcagtcctg gctgggtgaa cgacgtggcc ggggagatga
     4501 tctctgggat tacccggtac catgaactcc gaccccctct tcctcgtcat ggttggctat
     4561 ggggccatgg tggtcttcat gcttgtcctg tggggggttc aggtggtcca ccgcaatgcc
     4621 tcgatcgccg atgtcggctt ttgtgccgga ttgattgtcg tggtgctctg gtacgcccga
     4681 tacggattcg gggaccccga acggaagctg ttcgtggccg ccatggtctg cgtctatgcc
     4741 ggccggctcg gcctcttcct cttgttcaat cgtgtgatcg gtaaagccga agacgcccgg
     4801 taccaagaga tgcgtcgcag ctggggtgcc tcggagcaag ccaagatgtt cgggtacttc
     4861 cagttgcaag cggcagcggt ggccgccttt tcactccctt ttctggtgct cattcaaaac
     4921 ccgaggccgg cgttcggatt cgtcgagctc gcaggattct tcatctggat cgcggccatt
     4981 tcgggtgagg ccgctgccga ccggcaattg gcgcgatttc ggtcacgacc atggaatcgt
     5041 gatcgggtct gccgggatgg tctctggtat tactcgcgcc acccgaatta tttcttcgaa
     5101 tggcttcact ggtggagtta cgtcgtgatg gcgattggag cgcccggttg gcccctgacg
     5161 ctcattggtc cggctgggat gggttgggcg ctcctgcgtg tgactggtgt tcccttggcg
     5221 gagcggcagg ccctgaaaac ccgtggggcg gactaccgcc ggtatcagga aacgacgaat
     5281 gcgtttgtgc cgtggctgcc gaaacggtta cctgtcaagt ctgactgagc ttcgccagtc
     5341 tcgccttggc cttctccgct tcctgactcg agggatacag acgaattact tcttcatata
     5401 gctgcttggc gtgaggaata ttcatctggc gttcctcgaa ttctgccgtt tccagcaggt
     5461 ccttcgcctt gtcgcccgtg caggccatga cgaaaaccag aaagagcgcg gtgagcaact
     5521 gggtcatgtc gggtgtgctc catggttggg ggcccaccgg gaggcaagca ccgtgaggct
     5581 actcgagcca atgctgccac caggatgtct tccgaagcgt ctgcctcctc gcataggaag
     5641 cccctcgcat tttatcccag tcaacgacga ctctgccatg gtgcgcctca tgctgctgtt
     5701 gccagttttg gagcaggttc aagcaggcgt gatcgatgtg ccggagcgag gcgaagtcca
     5761 ggtggatttc tgcgtgcggc ggcgcaatct ccagcgtggc ggccaatcgg ggaagactga
     5821 cgaaggtggc gattccctgc agatggagcg tcatcttccc tgattcggta ttatgcgcat
     5881 aaaacgcttc cagattctgc gtcgtgtgca gaagtttcgc cagtgcgagg accaagccga
     5941 gcacgacgcc ggtcagtaaa ttcacgaaga cgatcgtgcc catggtcaca aagaaaatga
     6001 cgacttcgcc cctgccatgc ctcttcagtt ctggcagaat cccgaagttc atgagtttat
     6061 accctgtata gacgaggacg gcgcccaggc tcgccgtcgg aatgaaccgg agaaccgacg
     6121 gcagcaagga aacgaagagc agcaaccata cgccgtgcag gatcgcggat gctctcgtgc
     6181 gcgcgcccgc ctccacgttg gcggagctgc gaacaatgac ccccgtcatc ggcagggagc
     6241 cgagaaaacc gcagatcatg ttccccacgc cctgtgccag cagttcccgg ttgtagcggg
     6301 tccgagggcc ggtatgcagc tgatcgaccg ccgaggcgga aagaagcgtt tccacgctcg
     6361 caatgaaggc gagccccagc gcctccgcga gaatcgacgt gtcgaaaaaa tgagacaaca
     6421 tctggataga gggaaagtgc acgatggaca gcagattgtc ctgtacctcg atgcgctgaa
     6481 tcgggatatc caggagtgcg gaaacggcgg tggcagacgc aacgccgagg agcacggccg
     6541 gcatgacatg gaaccgcttg ggaagaacaa cattccacag aaccatcgtc ccgatggtca
     6601 ggacgccaat acgtgccgcc agatgatggt tcatcggcgt gctgtcgtcg tcgatcaggc
     6661 ctttccagac cgctaacgga agcgagagga tgttctcgag ccctgacccc ttgggcaagt
     6721 cgtcgatcat gatgtggaat tgactggcaa agatcagggc accgatgccc gccagcatgc
     6781 catgaataac ggccggcgaa acggcgcgaa accactgccc caactgcagc cacgaccaga
     6841 gtatttgaat gaaaccggcg gcgatcacga ttaccgcgaa ggcttccaag ccctgttcgc
     6901 gaatgagctc atagacaatg acggtcaacc ccgcggcggg tccgcttacc tgcaagggac
     6961 agcctgaaag ccacccgacg acgagaccgg cgacgatgcc cgtcacgatg cccgagccgg
     7021 tcggggcccc tgacgcgatc gtgatcccga tgcacaatgg cagggccacc aggaagacca
     7081 cgacggaggc caacccgtcg tgtctcaggc cgcctatcag gagggagaat gaggatggca
     7141 cagcggttcc gaggatgctg ttcataggga tctcgttgcg tgtaggctca ctttagtcga
     7201 cgaaggatga tcccccaagc aaatgctgaa aagccgcagt agtggcttct caggaggatc
     7261 catcgatgcc atagcggggt gcggtgatgc tttcgcttcg acagcgcggg cagcggcttg
     7321 gtctcgtcag cttcctgcgg tcctgaaaaa tgaaatggca ctccggacag gtggagggct
     7381 ccagaatgaa acgccgcttc cgatctcggg cgatcgtctt cacgacatga acgaggtggt
     7441 cctcgacttg ccgctccgag atgccgagca tctgcgcgag ttggtaggcg gaaagtctgg
     7501 ttccgctcaa cagctcgatc atccgctgtc gtgccgtttg tggtaccggc agcatccagg
     7561 ccatcctagg gaagaatgcg gtgaattcca agttgaacgg ctcacgtgac tttggcagga
     7621 tgaaggcacg atgacgcttt cagaccggga tgaaatggcg gaactggcgt tgaagcgcgt
     7681 gagggagtcc ggcgcggaat acggggacat ccgcctcatg cagagcatga gccaaacgat
     7741 cagcggcgaa gaccggcgta ttgcttcggt tcgtgatgtg gccgacagcg ggttcggcgt
     7801 gcgcgtcctg tatcatggcg ggtggggatt tgccgcgagt tcgattctgt ccctcgagga
     7861 agtgccgcga gtggctgatt tggcgatcga gattgccaaa ggatcggcct ctctggccag
     7921 cgagagggcg cggcttgtgg atgagccgat acatcgcgat cgagtcgtca ccgccaaccg
     7981 gatcgatccc ttcaccgtcc cgctcgatca aaagaccatg ctgcttcagg atgtgatgga
     8041 gtacgtgcag cgccaaccgg aagttgtgcg cagccatgca agtctgtggg caaggggcga
     8101 caagaaactg ttcgtttcga ccgagggcac acgtctggag tttgatttgc ttgcagtaca
     8161 gggagaattc acggcgactg cccggcatga tggccgcttc gcctcgcgat cgttcacgac
     8221 accgcacttg cgcacggggt acgagctcat cgagtcggca aattttatgg ccgaggcccc
     8281 tcgtatcgca tctcaggcgg tggaaaaagt caaagcgccg gccgtgcgac cgggcacgta
     8341 tgatctcgtg ctcgatccgg agcacctttc attgaccatg catgaatcct gtggccatcc
     8401 gagcgagctc gaccgggcgc tgggctatga ggcgaactat gccgggacca gctttctcac
     8461 gcccgagaag cgcggctcgt ttcagtacgg ctcttcgcaa gtcaatctgg ttgcggacaa
     8521 cacggaaccg gacacgctgg ctgctaccgg ttacgacgac gatggcgtgg cctgtcagaa
     8581 gtgggacatc gttcgcgaag gggtattcgt cggctattgc acgaaccgcg aggtggcctc
     8641 aaaaatcggc gaggcccgat cgaaaggttc aaaccgggcc gatagctggg ggagcgtgcc
     8701 catcgtgcgg attgccaaca tcggtttgga gcccggccag gcttcgctcc aagacctgct
     8761 cgccgacgtg aaacggggaa tctacattga aggccatggg tcctacagca ttgaccagcg
     8821 ccgctacaat tttcagttcg ggggcgatgc cttctggctc attgaaaacg gcaagcgcac
     8881 ccacatgctc cgggacgtca tttatcacgg catcactccg gagttttgga acagctgcga
     8941 tggggtcgcc gatcggcggt accgccggcg atatggcttc atcacgtgcg gaaagggcca
     9001 gcctggccag tcgggctgga tgacgcacgc cgcctcccat gcgcggttcc gccgtgccgc
     9061 cgtgatcaaa ggatgaaccg atcgtgagca tcacgactgt caaaccctgg cccaaaatga
     9121 ccgggcgcga tgaattccga tttctggccg accttgtgct caagcgttcg tctgcggatc
     9181 agacggtggt gaatctgtac gatcagcata gcgggacaac gcgtttcgca agcagccgta
     9241 tcatccaaaa tgtcgatacg aggcggggga cattgaccgt caccgtggcg ttcgatggcc
     9301 accggggatc tgcaagcacg acggatttct ccgccggctc cgtgcaagat acgctcgccc
     9361 gggcagagcg gattgcccgt gtgtctcccg aggatccgga atatctgccg ccaccggatc
     9421 cgcaaatctt tccaacgctt cagacgagcc ggccggagac ggccgcggcg ggtccgccgc
     9481 gacgtttgga atatgcaaac gaagccatcg gacagtgccg tattgaaaat cttgaagccg
     9541 ccggtattgt ctcctcttct gtcgcgtcgg tagggatcgc cgccagcaac ggcttgtttg
     9601 cacaggaaga gcgtacggag gcgcgtttca gtcttaccgt acaagcggaa gatgccaccg
     9661 gttggagcgc ggcggctcac cgctcgatcg atcatttgcg gatccaggat cggaccttga
     9721 cggcgatcaa caaggccaag cgaagccggg acgttcaaga actgcccccc ggtcggtact
     9781 gggtcatctt ggagccggcc gcggtggcgg gactgtggag ctggatgctc tggacgctgg
     9841 acgccaaatc ctatgagaag gggaccagtc ccttcgccga tcagctcggc cgtcagatcc
     9901 tggacgaacg actcacgcta cagaacctcc ccggacatcc cgacctgttg ggtacggggt
     9961 tcacccatga aggactgccg agcgaagacg cggtgtggat agagaagggc gtgttgaagc
    10021 agttggcgta tgaccgattc accgccaaac aacagcgaat caagcgcatc gcgacgctgg
    10081 aagcgccgtg cttgtccgcg tccgggccga cggcattgaa gacacaggaa ctgatcaagc
    10141 agtgcgagcg ggcgatcctt gtgacgaatt tctggtacat cagaaccgtc aatccaacgg
    10201 acctcacgct gacaggcatg acacgagacg gcacatttct ggtcgaggac ggccagatta
    10261 cgtccgccgt gcgcaacttt cgttttcacg agagtccatt gcgcgtgttc aggcatgtgg
    10321 acgcttgcac ggaaccggct gaagccgtca cctccgaaac gggcaaaatg ttggtgccgg
    10381 ccgtcatcct ccgggacttc catttttcaa gcgtcaccag attttgagga accatgaatg
    10441 acgagtgatc gagagttcga agcaggatgg ctcacgcggt tggccgccgc actgctcggt
    10501 cttctcatgt cgagtccacc gttgtacgcc gagacgcccc tgtacgataa tctcggttcc
    10561 ctgcatcacc ccatcactac cacatccgcg ttggctcagc ggtactttga tcaaggcctg
    10621 cggctggtct atgcattcaa tcacgaagaa gccattcgcg cgttcgaaga agcggcgaag
    10681 caggatccga acgcccccat gccctactgg ggggtcggat tggctctcgg tccgaacatc
    10741 aatgcggcaa tgtccaaaga ggacgagcgc agggccatcg aagcggtgaa acaagcacga
    10801 aaccgccttg ctgccgccag ccccgccgag cgggcctaca tcgaggccct gaccaagcgg
    10861 tatgtggcca cacgccgtgc tgcgcgcgcg tctcacgata aagcctatgc ggacgccatg
    10921 cgcaccgtgt ggcgagcgta tcctgacgac ccggacgccg gcgtgctgtt tgccgaagct
    10981 ctcatggacc tgcggccctg ggatttttgg acggcggagg gtaaacccaa gccagggacg
    11041 gaagagatcg tgtcgacact cgaggaggtg atcaagaagc accctgatca tcccggcgcc
    11101 tgtcattact acatccatgc cgtcgaagct tcagggaagc cggagcgcgc gctggcttgc
    11161 gcagaccgtt tgcccggctt gatgccgggt gctggccacc ttgtccatat gccggcgcat
    11221 atttatgccc gcgtcgggaa gtatcacgaa tcggcggtca gaaatgccga ggcggcgcag
    11281 gttgatgaac actacctcac gagtgcgcat ctgaccggcg attatgcgga cggctactat
    11341 agtcacaacc tccatttttt gtggtcttcg ctcatgatgg aaggccggag cgcggaggcc
    11401 ttacaggccg ccgcctcgct cgtttctacg atcacggagg atgaagctcg aagcaaccgc
    11461 agcaaagagc cctatctgac aacgaaactc tttgccttga tccgtttcgg gcgctgggaa
    11521 gagctcttac gcgagccgcc gccatcgaaa ggcctgcgac tggtcgaagg gatctggcgg
    11581 ttgggacgtg gcctggcctt agttggcaca gggcgattcc ctggcgcaga aggggagcgg
    11641 caggcgctca tgacgctgac gaagcagttc cgacggagca agaatcaaca ggacaagctc
    11701 gaaagagacc tactgaggat cgccgaacgg ctgctcgccg gacaaatcgc cgcccagcgt
    11761 cagcgttacg acgaagcgat cagggcattg aaagaggcgg tcgcccttga tgactcgtta
    11821 cagggtgcgg agccgccgca ttggccgctt cctgtgcggc agtatctcgg tcccgtcctc
    11881 ttgttgtcga gtcgtgctgc cgaagccgaa gcggaatata gggcggattt gaaatggtac
    11941 cccgaaaatg gatggagtct gttcggcctc ctgcagagtc tacaggccca gcggaaggaa
    12001 tcggaggctc aggagatcgc cggacggttc gaaaaggcgt ggacgcatgc cgatgtcacg
    12061 cttgcggcgt cccgattcta acagggcgtg acgtatgacg taccaggtcc ccattcaaac
    12121 cacgcgtttt ccaccgagcg gtactcactt tgacaagcct gggctcaaac cggctacaat
    12181 cgcctcattt ccgaacagga ggggcatgcg tgcgatatgt cgtgggcgtc atggggcctg
    12241 ccaaagcgag aagaaaagat gtggagaatg cacgagtgct cggggaactc attgctcggc
    12301 gcgagtgggt gctgctgacg ggcggccgcg atgtgggcgt catgcatgcc gccagtcagg
    12361 gggcaaaaaa agtggccagg agcttgacgg taggtgtttt gcccacgaca cgggacaaga
    12421 tttccaagta cgtggacgtg gcgatcgtca cggaaatggg cagcgcgaga aacaacatct
    12481 gcgtgttttc aagtcaggtc attgtggctt gcggtttggg cggatcaggg accgtttccg
    12541 aggtggcact ggcactaaag gcgggaaagg aagtggtgct gcttggggcg acgccggtcg
    12601 aggagacatt tttcaaaaat ctcgggggcc ggctggtgtc gagcgtacag actccggagg
    12661 acgccattaa tgtcattctc aggcggtatg aacggagcga tggagacgct cagcatccgg
    12721 cacaatcctg ggggcatttt tagctgcttg aacgacgcat ccgaagccac gcaccggccg
    12781 tagagtgatt gacccggccc atcgcgaccg atgcacgcga tgcgcgagat gaggataacc
    12841 atgacggctc tcacctgggc gatgcctatc ctattggtcc tcgggatcgt tgcaggcgaa
    12901 cagtcgactg tccatgaagg gcaggcagcc gaatccggca ccatggcaaa agcctatttt
    12961 gcaggcgggt gcttctggtg catggaggaa gccttcgaaa aggtggacgg ggtcctcgac
    13021 gtgctgtccg gttatatggg aggaagcgtc aagaatccaa gctatgaaga cgtgtcagcg
    13081 ggccgaacag gtcacgcgga atcggtcgaa gtacgctatg atccgagaaa actcacgtat
    13141 cctcaattgc tcgaagtctt ctggcgcaat gtcgatccgg ttacaccgga ggcgcaattt
    13201 tgcgatcatg ggaatcagta tcgagccgcc attttttatc agggggaaga ggaaaaacgc
    13261 gtggcagagg aatcaaaacg cgcgatcgaa cagtcaaagc ggttttcgac gccgattgcg
    13321 acgcaagtga cagccgcttc ggagttttat cccgccgagg aataccatca ggacttctat
    13381 aaaaagaatc cctttcgcta taaatactac aagttcacct gtggccgggc gcagcgcctt
    13441 gaagcgctat ggggaaagtc ttaacgacag cctgcgtcac ttcccatact gcatcaaaaa
    13501 tgacgcagcc tgtcgtactt tgaacagttc tgttccgttg ttaccaccca ggtacccacg
    13561 gttcctcctt accttttcgc agccttacgc aaacgagagt gcggcatgaa tgttgacctg
    13621 gttaggcggg atgagcccat gtcacacgga gaggctgcgg cgcatcggac aagcctcctg
    13681 caccgtactg ctggcgatgc tcatcgtggt cgtggtcccg accgcgacaa attccgactt
    13741 cgacagccac cagcactatt ccccgcagga gatccgtcgt gcgattgtct tctatgccag
    13801 acgctaccgg cttgaccccg cattgctgcg tgcagtgatg aaggccgagt ccgggtatcg
    13861 tcaacacgta gtttcaagaa agggcgctat aggattgatg cagcttacgc cggataccgc
    13921 tgcgacgttg cgcgtggcca acgtgtacga ttcgatggag aacatccggg gaggggccaa
    13981 acaacttcgc catctcctga acctctacgg aagcgacctt catctggcgc tcgcggccta
    14041 taatgcggga gtgcatcggg tgaagggccg tacggtgccc cggatacgcg agacacgtgc
    14101 ctacgtcagg aaagtgctca agtactaccg agccctcaag ggagagcagc gatgggttgg
    14161 accggtgcac ccagggccca aatccagggg aggtgtgaat ccaccggtga agtagggccg
    14221 cgtggacatc agtcctcggc cgtcatcaag atcgatcgca ggcgttcacg ctgagccggc
    14281 gaaaatgaat tccatgtcga aaggctgaca cccaccggac gaccgcatga tcgctccgtg
    14341 ccaccctcac ccaccccatg cttgcccctc tggtagtgtc cctagctctc ataccgaggt
    14401 atagagcacc gctctctagc cgcgactgtc ccatgtgcct cgcaagcatt cgagaagaaa
    14461 gaaatgtctg agctctctct gacccttgac gggcttagac aggctctgcg acaaaaggga
    14521 agggaaacgt caagtgacca aaagactgac caatcaacgg atggccgagc tgggacaatc
    14581 cgagattcgg gccatgacac aggcttgcgt cgcagccggt ggcctcaata tggctcaggg
    14641 cgtttgcgat acgccggttc cccccgttgt cttaaaaagc gccgcagagg ctatggtgca
    14701 ggggcgcaac agctattcgc ggttcgatgg actgccggaa ttacggcaag ccctcgcggt
    14761 caagttggac cgagacaacg gcatacgggc tgatccggaa acagacatca cagtcagcgc
    14821 cggggcgacc ggagcttttc attcggcttg tatggcgctg ctgaatccgg gagacgaagt
    14881 cattctgttt cagccgtact atcagtatca catcagcgcc ctgctggcgg tggaagggcg
    14941 acctgttatc gtaccaacga cggggcccga ctggacgttt gacccggaag cggttgaacg
    15001 cgccatcacg ccgagcacga gggcgattct cgtcaattca ccgggcaacc cctccgggaa
    15061 agtcttttcg agggaggagc tggaagtcgt ggcacggatc gccacggaac acgatctatt
    15121 tgttttcacc gatgagatct acgaatattt tgtctacgac ggccggcggc atgtcagcat
    15181 ggcctcatta cgaggtatgg cagatcgaac catcacgatc ggcggctatt cgaaaacgtt
    15241 cagtatcacg ggatggcgca tcggctacag cgtggccgcc tcccgatggg cgcaggcgat
    15301 cggcgcgatg aacgatctgc tctacgtgtg cgccccgacg ccgttgcaag cgggagtggc
    15361 ggccgggata caagaactgc ccgatgcctt ttatcgggaa ctcgccaagg agtaccaaca
    15421 taagcgagac cggttctgcg ctgcactggc cggggcgggg cttcccccct cagtccccca
    15481 aggcgcctat tacgtgctgg cagacgcgat caggcttccc ggcaggacag gaaaagaccg
    15541 cgccatgcac gtgctcaagc atgccggcat tgccggagtc ccgggcgagg cgtttttcgc
    15601 tgggctggac ggttcacgct acattcgctt cagctatgcc aagacagatg ccgatctgga
    15661 ggaggcctgc cgccgcatcg caggtcttcc gtgacccatg aaacgcgtca cttgtcctga
    15721 tcgcaaggcc gtgatatgaa aatgtatatg atcccgtcat catggaaaat agcattggtt
    15781 tccttgcccc tggttttgag cgtggcctgc accagcccgc ccgtcatgca cgaagagatc
    15841 ggacagccct ctctcccttc gacgtcaggc atccctcaag accggcgtgt gctggccgga
    15901 gagtgggatt acgaagatgg cgccgtcgtc acactcacgc tcgatgaaca gggaaacggt
    15961 tcctatccct ggaaagacgg ccggttcgtc acgcattccc tgtccgatca cgtctggcaa
    16021 gggatgtgga ttcagaaaga gaacgaccga gagggagggt tcaccgtgca gttctctccg
    16081 gatttttccg acggagaagg tcagtggtgg tatacgagaa ttgaagacga ccgggcgcca
    16141 acccaaaaag gcggcacctt ccgcttgacg agaaaaaatt cctcctcagc agcgaacgag
    16201 acctctcccg caccgtgaga tcaatgcacg agcgcaagca tgtccggttc gcagtgcaac
    16261 ggccggtctc ttttcgcggt gatctgctgt ccggtgaggg gaccatcttg aacatttcgc
    16321 gacagggctg cgccattctg tccgacacgg cgctcgagac tgaagcctat ctccaactcc
    16381 atctgcagtt gctcgagcag gagcagccgg tacatgtcca tttagctgcg gtgcggtggt
    16441 gtaccgccag gaaattcggg gtcgagttca tcaagatgca tcacgatgtc ggagaacgcc
    16501 tcaagcaatt tgtggagttg ctggaatcaa atgcatagcc cgcgttattt catcgcgacc
    16561 gttcgcacct ggtcataggt cgtcaggccg gcaatgactt tcaagatccc gtcctgaatc
    16621 agcgtgatca tcccggcctt cttggccagg ctgatcattt ctgctgtctt cgcccggttc
    16681 tgaatcaaat tcctcaactc atccgatcct ctcaaaagtt catgcagggc aacgcgtcct
    16741 ttgaatcccg tgtgattgca gtggtcgcag ccgcgcccgc gggaaagcat gaaggcgtct
    16801 ccgtaggggg ggacggcgag cgtatcccag gaattggacc cgtactcccg aaccagtttg
    16861 tcatactcct gcaccgtcgg atgatacagc tctttacaat ggccacaaat ccgtttgcag
    16921 agacgtttgg ccaggacccc ctgcatcgca tcggcaaagt tgaaggggtc gcaccccagg
    16981 tcgagcatgc gggttacggt ttcgaccgcg ctgttcgtgt gcaacgtact caagaccaga
    17041 tggccggtca atgacgcctc aacggctact tcggctgttt ccttatcccg catttcgccg
    17101 atcatgatca catccggatc ggctcgaaga aacgaccgca acgccgcagc aaacgtgaat
    17161 ccgattttgg gcagcacctg cacctgcctg agtccttcct gcgtgatctc gatcgggtcc
    17221 tcggccgtcc agattttcct ggcatcggtg ttcaggcgat gaagaactgc gtggagcgtc
    17281 gtcgtctttc ccgatccggt cgggccgacg cacaagagga tcccatgagg cttgtcgatc
    17341 acttccaaga gaccggcgag ggtggacggg gcgaactcca tggcttcaag cggcatgggt
    17401 tccttggcgg tcagaagccg catgacgatg tcctcgttat ttcccgccgt cggcatggtg
    17461 gcgactcgta cttcgatttc cctgtcccga ttcagccgga aacgcagctt gccgtcctgc
    17521 ggctttcttc gctcggcgat gtcgaggttc gacatgatct tgatgcgcga cacgagggcg
    17581 cggcggtacg cggccggaat gcgcatatag gtcgaacacg tgccgtcgac gcgaaggcgg
    17641 atggccgttt ccttgcgatc ggagtacggt tcgatgtgga tgtctgacgc ttcctggcgg
    17701 tacgcctcca caatgatctg gttggccagg cggacgatgg ccgagtcatt ttcgtcgatc
    17761 gcatccacat cgccggccaa ggactcttcc gcacgtgcct cgtgcacgag ttcgccgagg
    17821 gtgtcctcga tcgagccggt atccgtcatt ccaatcgtcg attgcaggaa gcgcttaatg
    17881 tcgcctttga gcgccactgc atagcggatg gtcatgccgg ggaatgcgcg ttggatgtca
    17941 tgccctctga cgatgtcctg cgggtcgtca atgagcacgt cgaggacgct gccgttggct
    18001 tttaacggca cccacacgtg tttccaaagg tagtcgtggc tcaagtcttt gatgagttga
    18061 gggtccacga ccatacgttc gtcgtagggg atgtaggggc attcgaaaaa cttgctcaag
    18121 gcctttccga tcgccgcttt cggaacgcga tattgctgga tgagcaaatc ttccagctcg
    18181 atgccttgct ggagcgaatc ctggatggct ttgatcagat ctccttgccg gatgaagctc
    18241 tcatcgatca ggagatcaaa ggcgcgtgga tcgatcgtct tgttgacgtt tctttgcacg
    18301 atcatgttga taccgtacac cctgcctgcc gggatacgcc ctgtgaggcc ccgcggatgt
    18361 tggccgagtg tgtggctcca gtctttggcg gtacgcccgg acaaggacca gtcattggtg
    18421 ataggtccga gtatacgggt ctcggaccga tcctcaacga ataagtgaaa agaaaccgag
    18481 ccgcggcggg atcttatccg ttgtgagggg agggaggcga accggagggc gggggatcca
    18541 gcaggtcata caacgctgaa taatcggctt cgccaagccc ttgtgcgatg gccgcctcca
    18601 aaagcgaagc catcccttcg agtggacgag tggtgagacc gcgagccccg gcctccctca
    18661 caaacagcct gacatccttg agcaaatggc gggtcgagaa gttcgggtgg tggtagtcgc
    18721 gcttgagcaa acgaggaagc ttcttgccga acgtcggggc atacaacgca ctctctcgca
    18781 agatcgtcat gaacgtgtcg acggaaatac cggcccgtcg gatgagccca aggctcaaac
    18841 caaaggccaa tgtctcggca gcgatcagct ggttgagcgc caatttgagt gcggcagctt
    18901 tgccgaccgc cccgacgaaa cgaggctcgc ggccgagcga ccgaaacagc ggtgcgagtg
    18961 cggcaaattg atcttcggtg cctccgacca tgactaagag cgttccggct ttggcttcgg
    19021 ccaaactccc caggacgggc gcctcgcaat atgtccctct ccgtcgtttc acttcccgtt
    19081 cgatctcaag gctttcgttg gaatcgatgg tgcccatttg cacgatcgtc tttccggaaa
    19141 gcgcagcgga gcaggcaggc gaaaacagga cagaatgaat ggctggtgca tcagtaagaa
    19201 gcagcaggac aaggtccgct tgagtgactg cgatctctgc ggagccgacc acggcaatgc
    19261 cctgcgcttc aagtggaagg gccttggtcc gtgttcggtt gaacacgacg accctgtgac
    19321 ccaccgccgt caagcgttcg gcgatggcct tgccgagcaa acccgttccg agtaaggcga
    19381 tcttcagcga agggagcatc accgcttctt tttcgtgcgc gcccggtctg cgtgcgtctg
    19441 tagcaacgga tgtaaatcgg ttggagcggt tgccgcaacc gcaatgacac cacggaatcg
    19501 gggaatcggc tggttgaagg tgaatgggcg gccctcggca tcggccacag acccgccgct
    19561 ttcctgcacc aagaggactc ccgccgcgag gtcccattca ttctccggtt cgatcgtcag
    19621 ggtcgcttgc actcggccgg ccgcaaccac cgccagcgca ttggcaattg aaaacatcgg
    19681 acggaaacgc atctggttat ccaggccggc ccaccgaccg gcgcgatatt cccaagggct
    19741 taccaggatg tgggacatcg catcccctgg agtctccggt gtatgcggtg aaccattgag
    19801 gcggacacct tggccgcgga tggccgtgaa caactcgtcc gtcgacggat tgaaaatggc
    19861 tcccagcacc ggaacgccgc cttgaatgag cccgaccgag atgcaatatt caggaagttt
    19921 gttcacgaag gccttcgttc cgtcgatcgg gtcgacgatc cacaccctgg ctttgtcgag
    19981 cctggccgga tcgtcggggg attcctcgga aagccacccg tcgtcgggaa agtgcatttc
    20041 ttgcatctcg cggaggatcc ggttcacttc gagatcggcg gtagtgaccg gcgagcgatc
    20101 tttcttggtg tgaatttcga agcccttgtg ggccaggtcc ttggccctgg cgccggcttt
    20161 cctgatggtg ctgctgagtt gggcgagttc tgattccatg gtgctgcgag cgatgcgtcg
    20221 tgatcagagt accaggtctt gaggtatcgg aaaagctcaa cctcgagtca cattcccctt
    20281 gaccgctcgt tcgaacctgc gtacaagctg gccagctcgg gaagacggct gcgggtgctt
    20341 tctggtaaca gcctgagaca cgaccacgta tgaagctggt tcaacagcgc tcaaaaaaaa
    20401 ccggcctgcc gccggggacg cttgtccaca tcggggagcg gaggaccgca aaagtcacgt
    20461 tcaccgtctt ccactacggc gcagccggct gcgaggaact gcagcctcat caagccgacc
    20521 agctggcgcc gcccgccgac gagtcggtcg tctggatcaa cgtcagcggg gtccacaacg
    20581 tcgaggtact cgaggcgatc ggcaagcagt ttgttctcca cccgctgttg ttggaagaca
    20641 tcgccaatac cgatcagcgg ccaaagctcg atgactacga aacatatttg tttctggtca
    20701 tcaagatgat gtccttgacg gatcggaaag agatcgccgt tgagcaagtc agtctggtgc
    20761 tcggacgaaa ttatgtcctc tcgttccagg aaaatggcac cgatgtcttt cagccggtcc
    20821 gcgagcgtct tcgaggagga aaaggaaggc tgcgtcaatc aggcgccgac tatttgttat
    20881 atgccttggt ggatgcgatc gtcgatcaat atttcgctgt cctcgaattc atgggcgaaa
    20941 aaatcgagct gctgcagcaa acggttgtcg cagatcccaa gcccgaaacg ctcaatgaga
    21001 tccacgggct gaagcgccag ctcctgtttg ttcgacgggc cgtctggccc ctgcgggatg
    21061 tgatgaacaa cctgtcgcgt tcggactgcc cgttcctcca ggaagccacc aagctgttct
    21121 tccgcgacgt ctacgaccat gtcgtgcaga tcgtcgatac catcgaaacg ctgcgcgaga
    21181 tggtctcagc cagtttggac atttatcttt caagtgtcag ctatcgcctg aatgccgtca
    21241 tgcgggtgtt gaccgtcatc accaccatct tcatgccgct tagctttatc gcaagcattt
    21301 atggcatgaa cttcgaacac atgccggagc tgaaatcgac gtggggttat ccgatcgtgc
    21361 tggtcgcgat gacggtggtt ggactgggga tgctgatctt gttcaggaac aaacgctggc
    21421 tctaaaactg cccgctctcg cccgcggcaa gccctgggca ccgccatggc ttgcccgcac
    21481 gaatcctctc ggcacgtaga cgctggcgtg ctcgatgtgt caggagtggg gcaggatgaa
    21541 gcgtccgact agtcgttttg aacctcaacg cgatccacga agtagggcgt gtcgccgaag
    21601 caggtcgtgg gcagataacg atattctttg taatgcagta cgacgcgctt tcccatcgcc
    21661 tctgacagct ggtccgccac agcatcgtcc catacggtaa attcccacag aaccggcgcg
    21721 gtgccgggga ccgtcgtcat ggccagttct ccctcatggg tcttacagaa ccatcctttt
    21781 ttggaaaatt tctgaacgta gccggcacga ttgccgtcag agtaactgta attaaaggcg
    21841 accagaagcg cgccggcggc cagaaacaag agcaccagga agaatcgggg tctaaacagc
    21901 cacatgggag cacaatcgcc gcctagttcg tgcggccctt ccagactcag gatggaaaat
    21961 cagagaacaa ccacggggcc tcagccctgc gccgggcttc ataggctgcg atcgccggct
    22021 cgtgctgtaa tgtcaagccg atggaatcca gtccgcgata caggcagtcc ttccgaaacg
    22081 gatcaatcga gaaaccgtac actgtgccgt ggggagtcgt cacggtctgc tgaccgagat
    22141 cgatcgtggc ccgatactgt ggcatcgcca gcacatcctt catcatcgac tccacggcgt
    22201 cggcttgcaa cacgaccggc agaatgccgt tctggaaaca gttgttataa aaaatgtccg
    22261 caaaactggt cgcgatgatg caacgaaaac cctgatccag taaagcccac ggcgcatgtt
    22321 cacgcgacga gccgcagccg aagttgtcgc gggtcaagag gatggtggca ccctggtacc
    22381 gcggctgatt caagaagaac aacggatccg gagaaccgtc cttctgcttg cgccagtcaa
    22441 aaaagaggcc ttcccggagg ccggtgcgtt tgatcgtctt gagaaactgc ttcgggataa
    22501 tctgatcggt gtcgacattg acccgatcca gtggggcaac gagaccggtc aattcagtaa
    22561 acgcttgcat caacgtcctt gcgtgagcat gtatcgggcc agccgctacg accagcgcct
    22621 gatgtcgacg aagtggcctt cgacggctgc agccacggcc atggccggcg acaccaaatg
    22681 cgtccggccg ccggcgcctt gacgaccttc gaagttgcga ttgctcgtgg aggcgcagcg
    22741 ctcccccggt tccagcacat cggcattcat ggcgaggcac atgctgcaac ccggttctcg
    22801 ccactcgaaa cccgccgccg tgaagatgcg atcgagtcct tcggcttcgg cctgctgttt
    22861 gatcaggcct gatccaggga ccaccatcgc gcggacggtc ttcgcaatgt gcttcccttt
    22921 tgccaggctg gcggcaagtc gcagatcctc catgcgagag ttcgtgcacg aaccgatgaa
    22981 gacgcgatca atccggatgt cggttatggg catgttggcc ttgaggccca tgtaggccaa
    23041 ggctcgctcg gtcgcctgcc gggttttctc atcgacgatg gttttgggat ccggcacctt
    23101 tccgtcgacg cccgtcacca tgccggggct tgtcccccaa ctcacttggg gggcgatgtc
    23161 ttcagctttc acgactacgg tggcatcata ggtcgcatcg tcatcggtct ggagatgctg
    23221 ccatgcccgg acggcctgat cccatgcagc gccggtcggt gcgagtggac gacccttgat
    23281 gtaattgaaa gtgtgttcat ccggcgcgac catgcccgcg cgtgcaccgg cttcgatcga
    23341 catgttgcac agcgtcatgc gcccttccat gctgaagcct cggatcacgg agccggtgta
    23401 ctcaatgaca tacccagtcc cgccggcggt tccgatttcc ccgataatgg cgaggatgac
    23461 gtccttcgca gagcagcgat ccgacagcac gccgtcgaca cgcacctcca tggtcttcgg
    23521 tcgcttctga atcaggcatt gcgtcgcaag cacatgctcg acttcgctgg tgccgatgcc
    23581 gaacgccaat gcgccgaagg cgccgtgcgt ggacgtgtga gagtctccac acacgatggt
    23641 ggtaccggga agcgtaaaac cctgctccgg gccgatcaca tggacgattc cctgccggat
    23701 gtcgctcatg ttgaacagcc taatgccgaa atctctgcag ttgtcttcca gcgtctgaat
    23761 ctggagggcg ctgacctggt ctgcgatgcc aaggcgccgg tccgtggtcg gcacattgtg
    23821 atcgggcacg gccaaggtgg cgcccggacg gcgaggtcgg cgaccggcga gcttcaatcc
    23881 ttcgaaggct tgtggcgacg tgacttcgtg aaccagttgt cggtcgatgt agagcagtgt
    23941 cgtcccatcg cgctcggctc gaacgacgtg ggattcccaa attttgtcga acagcgtctg
    24001 gccggccatc tcctctctac cccatgatcc tcatcttgcg aggcgtcatt atacagagac
    24061 tgcctatagg atgtgcaaga tcgtttgcct tgccctccag ggacgtgcta ccacgctgtc
    24121 ggcatgagtt ctcagccctg gatgaaccag accattgtgc agtggacgca gcggctgctc
    24181 gacagttacc ggcattggat ggggacagaa ttactcgaac gaaccggaga tcctgaactg
    24241 caagctcacg ccttgttcga gtcgccggtg gtcgtagtgt cccacggcat gcaaacggat
    24301 cccgtgttga attacggcaa caggatggcc ctcaaattgt gggaaatgac ctgggagcaa
    24361 ttgatcgaga ctccgtcgcg gctgaccgcc gagccggtca atcgtgctga gcgggaatgg
    24421 atgctcgagc aggcacgcgt ccgcggatat ctcgatacct atcggggcgt gagaatatcc
    24481 cgaagcggcc ggcgcttcct tgtcgaaggc gcggtcattt ggaacgtcct cgatgcccac
    24541 cggcaacaga tcgggcaagc cgcaacgttc gcccactgga cgtggctgtc ataacgactg
    24601 aggccgacat tacccgtcct cctcggaagg ctggaccggg aggcgggtga agcgcagaac
    24661 ggaacggccg tcgaccgtga ccgtatcgag aaacatcgct gcaggccgga cccaaagccc
    24721 tcgttcgcca tacaaggcgc gatacacgac gaaggattcc tccgtttccg aatgccgggc
    24781 aaccccaatg acttcgtagt catggccttt gtagtgccga tagcggccgg gcgtgatcat
    24841 cgtgtattct ttcgctgatc accatatata ggtgatgacg atgggaaagc aaacggcctc
    24901 actcacgttc gctttattgg cggccaccgg tctcgccgca atggtgccct cctgccagca
    24961 acagacctgg gaatcggtca tgaccgccgg ccaggaggca gtgcggcaag gtaactacgc
    25021 agaggccgag cacatgtttc aggtggccgt gcaaaaggct gagaccttcg gtcgcgaaga
    25081 tcgtcgtgtg gccgtcagcc tttcccagtt ggcgcaggtc tatgccggac agggtaaaca
    25141 cgctgaggct gaacccgtct atcttcaggc gctgaagatc tatcaggctg tacatggcga
    25201 gaatcatgcc gatgtcgcgg cgacattgaa caatctcggt gtgctccatc gtatgtacgg
    25261 tcagtatgcg gaagccgagc cattactgag ccgatcgctc gcgattaaag aacggctgct
    25321 gggaccgaac catcccgatg tggcgttgag cgtcgcaaac ttggggcagt tatacgtggc
    25381 ccagggtcag cccgcaaaag ccgagccatt gtaccgtcgg gcgttgtcca tcaagcgtga
    25441 ggctcttggg atgtcgcatc ctgaagtggc caagaccctt gaagatctgg ccaacgtctt
    25501 gcgcaagctc ggtcgcagcc ctgaggcggt ggctttggat ctgcaggcac gggagatccg
    25561 tgcgaagcgg agttgaagat gcgcggtatt ccgttgtagc gtatttgaat gccgaacgcg
    25621 aatcagccgc tctctccgaa ctggcgcatg agcgccatgg tggcgcgctt tgtcggaatc
    25681 atcgccatcg cgctcggttt catgttgggc atgtacggcc tggatgagca gggctcgcgc
    25741 tggctgcaca cggcactcgg actgttggtc acgggtatgc tcgctcaagg gtatgcgcta
    25801 tactgctcga tcaagcgtat gcaacaactt cacgaccggc ccaagacatg aagcgtgccg
    25861 gtagaacggt tccttccctt tccatctcgc tcctgctcgt gttcgccacc ctcgcagcct
    25921 gcggcggtcc atggcgcgac gggtatttca agaagggtgt caatcggctc acacaggacg
    25981 agatctcaga aaaattgggt cctccccaca cggccaagac tcccgctctg ggcggcgata
    26041 cgatctggac ctaccgagtc ccctacagcg acaaagacct ggatccgatg gacctgtccg
    26101 gaatcacgaa tgcggcgaac gaggccgcat cactgatcgg caaaggggcg caagatggtc
    26161 ccaagccgat gctctactgc tatcgctatg tcctgacctt caacgagcaa aaagtcctta
    26221 aacgttggaa gcgagaagag tgtgtacccg gtacccggga tactctgcat gccggctagc
    26281 tgaaggtcgc gttaggtcca tctatgatca catggcttcc ccaggtcgat agctcgatcg
    26341 tcatggacag cctgaagtcc ctcctgttgc tgttgtccgt tctcattgca cgcacgctta
    26401 ttgtccgctc gatctccagg aatgccgccc tcaccatgga agccaagcgc cggtgggtgg
    26461 tctccgtgcg taattcaatg gttttcgtct ttctcctggg tctcgtcgtg atttgggccc
    26521 acgagctgca ggcattcgcc gtgtccctgg ttgcgctggc ggccgctttg gtgctggcca
    26581 ccaaggagct tctcctctgt tggagtggcg cggcactgcg ggtcggcggc aaagtgtaca
    26641 tggtgggcga tcgaatccag attgcgggcc atcggggcgt cgtgctggat catgatattt
    26701 ttgcgacgaa gctgttggaa atcggtccgg ggcaggcctc gcatctctat accggacggg
    26761 tcacggtttt tcccaacagc ctgctcttca cgaacgcctt ggtcaaagaa aacccgaacc
    26821 aggaatacgg cctctatacg ctgaccgtcc cgctggcggc tggcgaggac tggaaacagg
    26881 ccgaggcccg gctgctcgag gctggtagag ccgaatgcgc ctccttcatg gcggaagcca
    26941 gccggcagat gaaactcctc gagcaacaaa atctcctcga ggctccgtct ccggaaccgc
    27001 gcattacgat tcaattgtcg gacgtgggcc gaatcgtgct cgtgctgcgt ttccccgctc
    27061 ctgaccgagg ccgatcgcgc attgagcagg cgatactgcg gcggtttctc ggggaagcct
    27121 gagacgtgtc ggcgcctcag ggtctgtcag tccggctctc ttgacagtcc gtgatgctcg
    27181 tgcgactctg ccccgttctg atgaccgatc gtgcaattca tcggcggatt tggagggtgc
    27241 ctatgcgaac catgctgacc gtggccgttg tcgttttatg gttgatgaat gtgactgtac
    27301 cggccgtcgc cgacgacgcc atcaccggcg agatgaatca actgcaagcc gacatcaagg
    27361 ccgaaaagga agcgatgaag gccgatacga agacgaagaa agccgagatg aagcagttga
    27421 agaaggaaca caagaaaaac atcaaggcca agaagcgaga gatgagagaa aagaacaagg
    27481 cgaataagaa caggatgaag gcgcagaagg atgccatgaa gacgcagggc caggatctga
    27541 atgcagtcgg tcaggacatg aaacctgccg ttccggatct tcccgcacca caagctcccg
    27601 gtgcacagta acctcggcta gccgtctaca gcagctgatc tgatcgcggg ccgctccagt
    27661 cggcccggcc cgcctgaatt cgagccaccg cctcttgtcg gctgcggaac gtcatgaaaa
    27721 tcgccaccta taatgtaaat tcgcttcgaa gacggcttcc tgtcgtcctc gactggctcg
    27781 agaaagaaaa acccgatgtc ttgtgcctgc aagaaaccaa agtccaggac agcgagttcc
    27841 ctctgatggc tctcctgccg tccgggtacg aaatcactta ccgaggcatg aaaagctaca
    27901 atggtgtggc ggtgctgacc cgtacaaagc cggaatcggt gcgggccggt ctcgacgatg
    27961 gtggagagcc ggacgagccg cgactgttgc atgtcgtcgt gaacgggatc gccatcatca
    28021 atacgtacat ccctcaggga ttcgagattg gttcacccaa atatgcctac aagcttgagt
    28081 ggttgaagcg gctgcgtgcc tactttgaaa aacatctttc gccgtcgctg cccgccatct
    28141 ggtgcggcga catgaatgtg gcgccgagag ccatggacgt gcacagtccg gaaaagcatc
    28201 tcaagcacgt gtgctatcat gaaacggcac gagaggcgta tgcgtcgacg gtcgcatggg
    28261 gattttacga cgtcttcgtg agactctatc cggagcgaca gcagttcacc ttctgggatt
    28321 accgggctcc aagttcgctg gacgccaaca aaggctggcg catcgatcac atgttggcga
    28381 ccgcatccct ggcggacaga tgtctgcggg ctgacgtcga cgtcgagccg aggcgggcca
    28441 gggatgcgtc agaccataca tttctttggg cggaattttc gctgccgtga cgtgacgttg
    28501 cttcttcaga tgttcaaacg ctcatcactc cacgccggct tgtcgcccgt cagtccggcg
    28561 agcactccat gaccactgtc tgatcaataa ggttcggttc gatccatcca ttgactcgaa
    28621 aggcaagtcg ctttcccctg ctgatgaccc gggaatacgg ggttaacgag cgtcgtgaca
    28681 gcggactctc cctatttgtt cgtgtcaaat atcgtgagat gagtcacgag ctggcaggtc
    28741 aacggtcaaa cggtcaatgg tgaatccgaa ggtgaggtca gaagcagatg tcgagccccg
    28801 ttgagcggaa tggtcgccag gttctaacca agcaaacgga gatctccggt caaaggcgaa
    28861 cgcgacatcc gcacctgccc tattcgacgg agtgcaagtc gttcactccg ccgaacgaga
    28921 ggatcacgcg ctgtcatgca gcatgcatcg ccgcgtgagt cgagcgggtg cgggcaagcc
    28981 ggttttgctc gatcagcgga tcaagaacca gtccgcagtt gatgcagcgc agcacagtac
    29041 cgtctgtgga gtaatccggt cgcgattccg cgatcatgag tcctttgcat ttttggcaag
    29101 tcatcttccc acctcctgca ttggatggca tggttgctcg agcgcgaatg taacaaccaa
    29161 agattaacgc ggtgttaatg gaccgttaaa accctctaat gtgaaacacc gatgaattca
    29221 ttaagaagat gctttttgtc aatcacttag gagaatatat ggcctggact aggggccgac
    29281 cgagtactgc tcttttgcga gggatggcct catagaacgt cgatcctatc agcctatggg
    29341 gttcctccat ttaatgaccg cctgtgccct tctgattggc tcgcacgtca tggcggcggc
    29401 gggaggatcg gtggcgatgg acaggaccga agtggccaga ggaaagcaga tcttcgccaa
    29461 gtactgcgcc ggatgccacg gatcccaagg aaagggcgat ggctatcaac tgcttggtcc
    29521 cagtccggca aatctgatat cgccgacgac agaagaacgg tccgacgacg acttactccg
    29581 cacgattcat gaaggtaagc ccaatatgcc ggcatggaag catcggcttt cgcgtcagga
    29641 tagccgggat gttctcacct atatccgctc cctgtcggcc gtgcaaagga agtagggtgg
    29701 ggataccgtc gggccgcgat gacgcgacaa aaccgaacgc ggccaagccg gcgcgcacca
    29761 tacgaaccag cgctgaacac tatgaagggg ttggctgtcc tgaacgtacc accaggaccg
    29821 gacatgtgac ctggtgcacg atcgcatggg agacgctccc caggagaaac cgatccagcc
    29881 cttcccgtcc gtgcgaaccg accacaacga ggtcgaactc cttcgcagtt tccgagatca
    29941 caggtgccgg ctcccccttc atcacacgac tcgacgcact gtagcgtgac gggagcagcg
    30001 cctcggcgca ggctctgacg cttgcctgcg cttgggccat caattcctct gaccaggatt
    30061 cgaatggcgg gataggagag agttccggca tcggcgtcgg gagcatcggc acggccgtga
    30121 gcaccgttgc ttctaccgaa gtgcgaaacg gatggcggct cagccacgcg cagatttgac
    30181 ggccatcgtc acgtccctcg atggcaacta acactcggcg gatcggcttc gcatcgcctt
    30241 tgacgagcaa ggtcggctgc atcgcgtgca acaggacacg gtgggagaca ctggtgagca
    30301 ccagttcggc cactctcgaa tgatcatgcg tgccgacgac gatcaggtcc gcggccatgg
    30361 cggtggcagt atcgaggagg aaccgtgccg gtttacgaaa ctcgcagagt tgacgcggct
    30421 tgggaatatc gttcggcagc atcgtcgcgg cccggtccag cgattcgcag ccggcctcga
    30481 acatcgattt tcggaagata tcgtacccct ggagattggc ggcttcggcg acgatggggt
    30541 attcaaagag tcccaaatcg acgccatgca ccagcgtgat gtctgtcggc tgatagagca
    30601 aaccgagctg ttcgaccgcc gcgaaagccg cgtcagacca gtctacgcct gcaacaatct
    30661 tcatgtctca ctccctgtgc acgccggtgt ctcgtcaacc actggccgtc tgcgcgctct
    30721 ctgattgtct tgccgcgttc agataggcca ggacctgcgc atcatccacc tgcgtaaaat
    30781 tatggtagtg atggccgacc gccatgaacg gctctggcgt tgcgatcacg atcaattcgt
    30841 cgacgcgatt ccgcaccgat gggaggctat ccaggggcgc gacggggatg gcagcgatga
    30901 gtcgaccagg agacaatttc ctgacacctt cgatggttgc taagaaggtg gcgccggtcg
    30961 cgatgccgtc gtcgaccagg ataacggtct tgtctcgaat ctgggcaagc ggcaatccgt
    31021 gacggtacag ttgttgccgt cgcgcgagct cctgccgctc attgtcgatg atcgtttcca
    31081 gatcggcgtg ggaaagacga aaagcctcga ccgcttcagg attcagatag agagcgccgg
    31141 tttcgctgag cgcaccgatt gcgtactctg gattgtccgg cgtactcagc ttccgagtca
    31201 cgaacacgtc gagcgccaga cgcagagaaa cgctcagttc gtatccgact gccactccgc
    31261 cccgtgggag cgccaaaata agtccgctgg ggtcgcctcg gtaggattcg agcttctccg
    31321 ccagtagtcg gccggcttct gctcgatctc ggaacaccac aaccacctcg ttcaatggtg
    31381 ttctcttcac aggaggaagt tatgtcactg ttttacacag gtgtcgccgg caatggcgac
    31441 aggtgagatc cctggcaccc tcagcggacg gagccggtct cgaaagaccg ggaagaccta
    31501 agcggacggc atacctcgag gagaggatct gggacattgg ctcgccaagc agggcgccga
    31561 cgatgctatg caaatgacgc tgcgttgtgg cggacggatg gggcagccgg atgcacatgc
    31621 cgatgcatcg ggccataggt gcacatggca aacacgatac ttctaaagag atgatcagcg
    31681 gcatcctcca tccgtacgaa ggccactgct cctgccgcaa tcatcgcgtt atagaggtac
    31741 ggcgtatact ctgcggacat tcccaaaatg accacttgag gaagaatctg cctgatctgc
    31801 cgagtcgctt ccactccatt cgcaagatga gcatccatca gcaccaggtc gaccgccatg
    31861 gctttggcct gctcaaccgc ctcgtccgca tcagcagctt caccggcgac ctcaatgaga
    31921 tcggcatagc cgtccaagat catgcgaatc atttcccgta tcgacgaatg ggggtcgaca
    31981 attaacactc gccatcgaag gccagcctca gtcttgtttt gaacagacga tggatgacgt
    32041 tcaaacggct tggttagggt aatggttggc atgtgaccct cctcggctgg cagtggctgg
    32101 atgcgtgtac gtaccccttc cacatttttt cgataactag ggaggtccct agtctgtgta
    32161 ttctgatact taccaacccc ttagcggtcc tttgaccgcc gctcggtgag aatgttacac
    32221 tgtgtcgccg tacgagctat atgttgtaac ctcgcgctct tcccctccta ttacctgtgt
    32281 tgcgcgataa gtcttaaagt tgctcctacg tgttgcgggt gccgatcgga agcaaagaat
    32341 cgggttcgga cgacgctttc tcattcggat gccgaaatct gaaactgtta gcgactgatg
    32401 aaaggaatgc cgatgaaaat ctcgcatgtg tggaagtccg gtagcgccaa gaagaaaacc
    32461 aagccgacct acaagatccg ttgggaactc ttaggattgt cccgtccgga tcaaatgcaa
    32521 tcgagtctct ctatggacgc aatacgaagc gaggtctcgc ttctggcaac acaaggtttg
    32581 agcgaaagga ctcagtcccg atcgactgga cccgccgtca gtggacgagg ccacaagcga
    32641 agcatcactg gaagccggcg tcggggtgct gaaaagtaac aaccagtttt gtaacgaagg
    32701 agggttacca tcgcacgagc aggaaagaca acgctagcca aacgcgaccg cgaaaaagct
    32761 ttgcaacagc agcgtcggga caaagaagcg aaacgagtgc tccgcaaagc cgagaaagcc
    32821 gcgcgtagca ccgctcctga aggtgaagat ccggatttgg ctgggcttca atggggaccg
    32881 cagccgccgg cgttttaagc tgagattccc tcatggcgac tgcctcgggg cagctcgcct
    32941 gaggcgagtc acaacactcc acgtagcctg ttcccatttt gcagaaagac ttgcgcctgc
    33001 ttcctcatgc ctcttacaat cgcccccgct gagaggcgtt catgccgtca ggtttgtccc
    33061 gttggttcat ccgcccatcg tactcccact cgttgtctcc aaccatagtg gtcgacaaga
    33121 gatcgcggac caaatgtgtt ttcgtcgggt tcctgctgtt gatcgccgga tgcgccgtgc
    33181 tgccgggcca ttggcggccc ggatggaacg agcactgggc agacaacaag ccggtgcccg
    33241 ataccgccgg ctcatggaaa ttgaccgaac ttgaagtcac agacggtcgt tttctggcgc
    33301 tagccatctc gggcggtgga agccgcgctg ccaactttgg cgccgccgtg atgctcgaac
    33361 tcgagcatcg caagctcttg gagcaggtcg atgtgatttc tggggtctcc gggggaactc
    33421 tcccggccgc atattacggg ctgggcgagc aggcgggcgc cttcacggag tctgcccttc
    33481 gaaaagcgct gggattcgat tttcagggca gttggatcag gcgatggttt ctgccgcaca
    33541 acatttttcg ctattggctc tccgacttca cccgttcgga cattatggtt caggtgttca
    33601 acaaccaact ctatcacgac gccacgttcc ggcacctcct gccgcatcca aaaatcttgc
    33661 tcaattccac ggttcacaac gatcacaccc gatttacgtt tacggacgag cgcttcgcta
    33721 aactccattc agtgctggcc acctatcccg tcgccaacgc cgtgaacgca tcgtcagcat
    33781 ttccgggtgc gtttcaagat gtgaccctgc agtggtacat ggaaccgccg caatacatgc
    33841 atctgtatga cggcggtccc atcgacaatc ttggcgtgca ggcgattacg gagtacatca
    33901 atcgaaacat cctgggcacg tcccttgatc gattgtttcc caacggttgc ctgatcgtca
    33961 tcattgatgc cacgccggcg agcgagcatc ccgagctcaa cgcggggaag tccagccgct
    34021 cgttgattga ctacttcgtc gataccaatg cgttggatgc gatggatgcg ttgctcctcg
    34081 agtccaggcg gtccttgctg gcaggcatgg gcattcccat
//
LOCUS       DASSCQ010000200         9560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1810576, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000200 DASSCQ010000000
VERSION     DASSCQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810576"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..1224
                     /locus_tag="VFH05_12830"
     CDS             415..1224
                     /locus_tag="VFH05_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET6679153.1"
                     /translation="MSLESQPATSSPPPNPLGGKTLIVDWQDPNVYPRPSAALKDAGD
                     QDQVFVRAGIYEDKLFISARPVLLTGAGRDLVQIYSRLGGPLYLQRVPSGRISGLTFR
                     YVGSDQHSAINVLDSSCMITSCRATEGILSGVVIYGPECRPAFIDNEVSGNRESGIFV
                     FAGAQPRVADNVCRGNHHFGIAVRDRGSRPELVRNRCSDNWLSGMLFFHHAEALLVDN
                     VCTDNVHWGVVATPDSRLSPPPDGLAGSNVLVPNPRGALHITDEPLSEIGR"
     gene            complement(1266..2195)
                     /locus_tag="VFH05_12835"
     CDS             complement(1266..2195)
                     /locus_tag="VFH05_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HET6679154.1"
                     /translation="MISVLLIEDNDVDARLTQDLLAEWSVEEFKVTHAKTLSDGLAYI
                     DKGRFDAILLDLSLPDAFGLPTVRQVHATNPSIPVVVLSGVSDQSLALQAVQQGAQDY
                     LVKGQGHPELLARSVRYAIERKRSEERLTYLAQYDQLTGLVNRTLFRDRLIQAMARSK
                     RLQQPLGLMLLDLDRFKSVNDTLGHDVGDELLKAVAERLKGCVREVDTVARMGGDEFT
                     IILEGVASEQSIVVVAKRITESITSVFPLKGHTVSVGISIGITVYPHDDHSIDELLKH
                     ADSAMYRAKQQGGSGFAFHEASGYNHAATYSAS"
     gene            complement(2417..2794)
                     /locus_tag="VFH05_12840"
     CDS             complement(2417..2794)
                     /locus_tag="VFH05_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679155.1"
                     /translation="MEDLVIEEEGASSALKITLGDFLVFINWMNQTEKSLERGEALPT
                     TPDLDSGKVKGWIQLVQRLEERFRRSEEKKIALEANVIAQEAQLGQLLTHLHANIASL
                     YDNLGQAKKAEEVRRRAEALHAT"
     gene            complement(3066..3389)
                     /locus_tag="VFH05_12845"
     CDS             complement(3066..3389)
                     /locus_tag="VFH05_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679156.1"
                     /translation="MTGRLVMLLSCILLSGCAEPFTVFHSQTGEQILLGRRSFSYDDC
                     LSSIREEANRLGMTLRYVHVRGSAIGRSLLWPFEPGYACEAAAGPEGHPNGIYYNVPP
                     RVPQG"
     gene            complement(3427..3765)
                     /locus_tag="VFH05_12850"
     CDS             complement(3427..3765)
                     /locus_tag="VFH05_12850"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6679157.1"
                     /translation="MKIRYSERVLTDFPVIFAGDAYVGEGVVNNVSVPGCEILSNKSV
                     EPGTYLEMKMMMPDSGPSLSVGLAKIRWCRGRRFGVEFIRMPGGDQVRLGRLVKQQRA
                     FRSTARLHHS"
     gene            4014..4664
                     /locus_tag="VFH05_12855"
     CDS             4014..4664
                     /locus_tag="VFH05_12855"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6679158.1"
                     /translation="MTKNEKQSGSSQLISVLLVLVGTVSLGISTPSTVADSGFYGRVP
                     IFHEPMEYRDISHVPARTPDNPMASGTELQHAVTRAARNHRLHPALLYAVMKAESSFN
                     PLVVSKAGAVGLMQLVPETAMRHGVRNLYDTNENVAGGARHLRYLLDRFHGNTRLALA
                     AYNAGEGKVDRYQQIPPYKETRHYVQKVLTYYREYRREVPIAAAEGHSGGYTIRAL"
     gene            complement(4699..4899)
                     /locus_tag="VFH05_12860"
     CDS             complement(4699..4899)
                     /locus_tag="VFH05_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679159.1"
                     /translation="MNFIRIGKKALNLDQVTYAEAQVWQDEMSVKVYFVGSANNAPVV
                     FAENEAKELWKYLEYVAEKPVV"
     gene            5110..8253
                     /locus_tag="VFH05_12865"
     CDS             5110..8253
                     /locus_tag="VFH05_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET6679160.1"
                     /translation="MNQLSVLIAEDEAILAAHLASKVKLLGYRVIGPVSSGEEAIELA
                     EKEGPDLALLDIRLDGQLDGIQTAQRLKRWRDIPVIFLTAHSDHETIKKASSIGPFGY
                     ILKPFEERDLATQLEITFYKHQAEASLRKSEERYRAFIQNSSEGIWRMEFDPAVDVAL
                     PVEEQVERIYDSARLVECNDAMGRMYGFSNAEGLIGKTLDFMLPASDPSAREFLASIV
                     RAGYRATDMESMERNVFGETVYFLNSMVGIVHDGFLVRMWGTQQNITDRKCAEEAIRE
                     SEARTRIAQQAARWGVFEYNYKTGKNYWSVELEALYGLRPGSFEGTYEAWLGRVHPND
                     CIDADRAMNQALSTNEYTHDFRVIWDDGSVHWLFARAKIFRDANGAPERMLGVNVDIT
                     ERKQAEEALRRSEEQLELISDTVPALISYVNRDRQYVTCNEAYTKWFGLSRNDIVGKT
                     MQEVVGAEAWGAMAAYVEAALQGQTVDYETEARYRYGGTKWIHAVYTPHRDAEGHIAG
                     FIVLVTDISARKRTEAALRESEMRFRTIADSAPVLIWMNGLDGCEFVNHAYLEFIGSS
                     QQKDVLNYEWAQYIHDDDRESYLQLYFDAMRDHRPFDSVCRFRRFDGQYRWMRSVGRP
                     RLAPNDKLIGYVGANVDITDIQEAQDRLQRWSDELTKAVSLKTHELLESQDRLRSLAA
                     EVGRAEQRERKRIATELHDHLQQMLVFCKLRLGQGRQQAAAIPACAEVIKQVDDVLSE
                     ALTYSRTLVAQLCPPVLQEFGLAAALQWLGEQMSQRKLHVSVHVEHIPVPEEQAVLLF
                     QSVRELLMNVVKHAGTAYASVEMQRDKKALRIEVRDSGSGFDPAVVQLYAGAQPPKFG
                     LFSIRERMKAFGGMLEVETGLGKGTSAVLLLPLEETKNETSGSPVYDQPVAVGQLKPG
                     AREMPRPSEKHRLTVLLVDDHAMMREGLRSVLEAHSDIHIVGEASDGQEAIALVDELR
                     PSLVVMDINMPRLNGIEATARIKARYAEIKVIGLSVNAGPNNKEAMLRAGADMLLTKE
                     AAVEELYRSIQEVMPRI"
BASE COUNT         2231 a         2495 c         2711 g         2123 t
ORIGIN      
        1 gtgcggcttc ataatcccgg tgtttgaact cttccatcac gcggtcgtaa agatgacgag
       61 aagtgtcctg aacttgtgct ggagcggcca gggtccccgt gggctgtccc gcgatcagga
      121 ccagacatgc gagaattccc agtggaacac gtgcaagagt tttcatcgat ccgcctccat
      181 ggtccgcggc ctctgccaag gcacgacacc gagttcgcaa tccacatgcc acggcaaggg
      241 ccgccggtca gagaaaaacc ggtgcaatat cgggtgcttt cctcgtgcgt tcgaacggcc
      301 cattgatcga tacaaggcct tacacgacca gttttgtacg tcccgcggct tgacaagctc
      361 aaacgggttg ttccacatga tggggcgaaa acgctgattt aggcgggagg agcgatgagc
      421 ttggaatctc agccggcgac gtcaagtccc ccgccgaatc cactcggggg aaaaacatta
      481 atcgtcgatt ggcaagatcc taacgtctat ccacggccga gcgcggcgtt gaaggacgcc
      541 ggtgatcagg accaagtgtt tgtcagggcc ggcatttatg aggacaaact gtttatttct
      601 gcccgccccg ttttgttgac gggggcgggg cgagatctgg tccagatcta ttcacgactc
      661 ggggggccgc tctatctgca gcgagtcccc agcggccgca tttctggtct gacattccgg
      721 tacgtcggaa gcgaccaaca ttcggccatc aatgtgctgg attcttcctg catgatcaca
      781 tcgtgccggg ccacagaggg cattttgtct ggcgtagtga tttacgggcc tgaatgtcgg
      841 ccggccttta tcgataatga agtcagtggc aaccgggaat cgggcatctt tgtctttgca
      901 ggtgcgcagc ctcgtgtcgc agacaacgtc tgccgcggca atcatcattt tgggatcgca
      961 gtacgggatc gggggagccg tcccgagctt gtacgcaatc gctgctctga taactggttg
     1021 agcggcatgc tgttcttcca ccacgctgaa gcgctgcttg tagacaacgt atgcaccgac
     1081 aacgtgcatt ggggcgtggt ggcgactccg gacagtcgtc tttcgccccc tccggatggt
     1141 ctggctgggt cgaacgtttt agtgccgaac cctcgcgggg ctctgcacat cactgatgag
     1201 ccgctttcgg aaatcggacg ctgagagcgg ccaatgccta gactgtgtca ttggaatgcg
     1261 gagggtcagg aggcggaata ggtggcggcg tgattgtagc ccgaggcttc atggaacgca
     1321 aagccgctgc caccttgctg cttcgctcga tacatggcgg agtcggcatg tttgaggagc
     1381 tcgtcgatgc tatggtcgtc gtggggatat accgtgattc cgatactgat tccgacggag
     1441 accgtgtgcc ccttgagcgg aaagaccgac gtgatggact cggtaatccg cttcgcgacg
     1501 accacgatac tttgctcgga cgcgacacct tccaaaataa tggtaaactc gtccccaccc
     1561 atgcgtgcga cggtatcgac ctcgcgtacg catcccttca atcgctccgc aactgctttg
     1621 agaagctcat ccccgacatc atggccgagc gtatcgttca ccgacttgaa gcggtctaaa
     1681 tccagcagca tcaggcccag cggttgctga agacgtttac tcctagccat ggcttgaatt
     1741 aagcgatcgc gaaatagcgt tcggttgacg agccccgtca attgatcata ttgagccaga
     1801 tacgttaatc gctcttccga gcgcttccgc tcgattgcat agcgcaccga tcgagccagc
     1861 agctccgggt gcccttgacc tttgacgagg taatcctggg ctccttgctg cacggcctga
     1921 agggcgagcg attggtcgct caccccgctc agcacgacga cgggaatgga ggggttggtg
     1981 gcatgaactt gtcgcacggt tggcaggccg aaggcatcag gaagcgatag gtcgagcaga
     2041 atcgcatcga agcgcccctt atcgatgtac gccaggccgt cggagagcgt ctttgcatgt
     2101 gtgaccttga attcttcgac gctccattcc gccagcaagt cctgggtcaa gcgggcatcg
     2161 acgtcattgt cttcaatcag gagaacgcta atcatcgtgc aaaaatccgg ctgtaaacgc
     2221 tcgacatttc aggccgatgt tgcagattga aagagcctgg accgctaccg agtcgattat
     2281 aacgaacggc tgctgggcgc gaagcaaaat gtggaattac tcatgccggg gcgtcaaggg
     2341 acaccaatcc ggtcactcag ggcgggaaaa aaccgtggtg aatgagaaac ggtcaaacgg
     2401 gggacccagg atcccgtcag gtggcgtgca acgcctcggc gcgccgacgc acttcttcgg
     2461 ccttcttggc ctgcccaaga ttgtcgtaga gcgacgcaat gttggcatgc aaatgcgtaa
     2521 gtaactggcc gagctgcgcc tcttgtgcga tgacgttggc ttcgagagcg atcttttttt
     2581 cttcactccg gcggaaacgc tcttccaaac gctgcacgag ttgaatccac cccttcacct
     2641 tccctgaatc cagatcaggg gtggtgggca gtgcctcgcc tcgttcaagc gacttctcgg
     2701 tttgattcat ccagttgatg aacacgagga agtctccgag ggtgatcttc aatgctgagg
     2761 aggcgccttc ttcttcaatc acgagatctt ccatgatgac tcctttgaga tgatgagacg
     2821 acgaatgagg ccagtcgatc caagctgtga cggacgatga cagagttggc gcctggctcc
     2881 acgcaggccg gctcctttca ggtgagcagg gaaagttttg gcggatgcac gagcaggtgt
     2941 ttcatcaggc gtgatactag cgtcgtgcag tcatactgcg attgcggttc gcttgtcaac
     3001 aacttcaact cccattgagt cgaattttac atgttgtgtg gaacgaggaa ggcaagaagc
     3061 gagatttatc cttgaggcac ccggggaggc acattgtaat aaatgccgtt ggggtgtcct
     3121 tcaggtccgg cggcagcttc acaagcatac cctggttcga aaggccagag taaagaccgg
     3181 ccgatcgccg atccgcgcac atgcacatag cgaagcgtca ttccgaggcg gttggcttcc
     3241 tcacggatgg atgaaagaca gtcatcatag gaaaaggaac ggcggcccag aagaatctgt
     3301 tcgcccgttt ggctgtgaaa cacggtaaat ggctccgcgc agccggagag cagaatgcaa
     3361 gataacagca tgacaagacg accggtcatg acgagagctc caagaaccta aaaggaagtg
     3421 tagcattcac gagtggtgta gtcgagcagt cgaccggaaa gcacgctgtt gcttcacgag
     3481 tcggcccagc cggacctgat cgccacccgg catgcggata aattccaccc caaaccgacg
     3541 tcccctgcac catcgaattt tggccagacc tacgctgagt gatggtccgc tgtcaggcat
     3601 catcattttc atctcgagat aggtgcctgg ttctaccgac ttgttgctga gaatctcgca
     3661 cccaggcact gagacgttat tcaccacgcc ttcgccgaca taggcatcac cggcaaaaat
     3721 gacagggaaa tcggtgagga ctcgttcgct gtagcggatt ttcatgcctc gccctccatg
     3781 ctccgatgac gggactactt tgtcaaagac gacaaattgc gcttgtcaga cattataggg
     3841 aatcagcgca gcgtgccatc cctacaaaag atgggagtga acgaattgtc taagtcatta
     3901 ttttattgga tgaatgtctt cattttaagg tctcgtcctg gggcacgaaa actgcggttg
     3961 ggtaaccagg tggacagttg aatgaaatga gtgtctgcct cttggaggac gacatgacca
     4021 agaatgaaaa acagtccgga agcagtcagt tgatctctgt gttattggtc ctcgtcggca
     4081 ccgtgagctt aggtatctcg accccctcta cggtggccga cagcggattt tatggacggg
     4141 tcccgatctt ccacgagcca atggaatatc gcgacatttc ccatgttcct gccaggactc
     4201 ccgacaatcc aatggcttca ggtacggaat tacaacacgc cgtgacaagg gcagcccgta
     4261 atcatcgcct acatccggcc ttgctctatg cagtcatgaa ggctgagtct tcgtttaacc
     4321 cgcttgtcgt ctcgaaagcc ggggcggtcg gccttatgca attagtacca gagacagcca
     4381 tgagacatgg cgtgcggaat ctatatgata ccaatgagaa tgtggcagga ggcgcccgac
     4441 atcttcgcta tcttctcgac cggtttcacg gtaacacgcg tctggcgttg gctgcctaca
     4501 acgcagggga aggaaaagtc gatcggtacc aacaaattcc gccatataag gagacccggc
     4561 actatgtcca gaaggtgttg acctactatc gtgagtatcg gagagaagtt ccgattgctg
     4621 cagcggaagg ccattcagga gggtatacga tacgggcctt gtgaggcgcg gaagtcagag
     4681 attgactgat cgggctggtt ataccacagg cttttccgcc acgtactcca agtacttcca
     4741 taattccttg gcttcgtttt ctgcaaaaac cactggcgca ttgttcgccg aaccgacaaa
     4801 atacactttc acgctcatct catcctgcca gacctgggct tcagcataag tgacctggtc
     4861 caaattcaga gctttctttc caatccggat gaagttcatg gatacccctt taggtaagta
     4921 gccttcgaac tatagcatcc gtcgtgagtc cgtcaatgag ttacccattt tctgcccgca
     4981 atacagaaaa cttgaatgag cccccccgtg agatcgagcg ggggacgaag ccagggtttc
     5041 ccctaggccc ttgtaagacc gactcccact tatacttgcc ccgcttttcc tatccactgg
     5101 ttggtcggta tgaaccaact ttctgtccta atcgcggaag atgaggccat cttggcggct
     5161 catctcgcca gcaaagtcaa gttgctcggc tatcgtgtca tcggtccagt ttcctccgga
     5221 gaggaagcca tagaactcgc tgagaaagaa ggtcccgacc ttgccctcct ggacatccgc
     5281 ctcgacggcc aattggacgg aattcaaacg gctcaacgct tgaagaggtg gcgggatata
     5341 cctgtcattt tcctgactgc gcattcggac catgagacca ttaaaaaggc cagttcgatc
     5401 ggtccatttg gctacatctt gaagcccttc gaagagcgag acctggccac tcagctcgag
     5461 atcacgttct acaaacatca agccgaagcc tcgttgcgga aaagtgaaga acgatatcgg
     5521 gccttcatcc agaacagctc ggaaggcatc tggagaatgg agttcgatcc ggccgtggat
     5581 gtcgctcttc cggtagagga gcaagtcgag cgcatctatg attcggcacg tcttgtcgaa
     5641 tgtaacgatg cgatgggacg catgtatgga tttagcaatg cagaggggct catcggtaag
     5701 actctggact tcatgctgcc cgcctccgat ccttcggcgc gcgagtttct cgcttccatt
     5761 gtgcgggccg ggtatcgggc cactgatatg gaatcgatgg agcgcaacgt attcggggaa
     5821 acggtgtact tcctcaatag tatggtcggc attgtccacg atggttttct cgtacgaatg
     5881 tggggcacgc aacaaaatat caccgatcga aaatgtgccg aagaggccat tcgtgagagc
     5941 gaggcacgca ctcggatcgc gcagcaggcg gcccgctggg gagtattcga atataactac
     6001 aagaccggaa agaactactg gtctgtcgaa ctcgaagcgc tgtatgggct ccggccgggg
     6061 tcgttcgagg gcacctatga ggcctggcta gggcgtgtcc atcccaatga ttgcatcgat
     6121 gctgaccgag ctatgaacca ggcgctctcg accaatgagt atacgcatga ttttcgggtt
     6181 atttgggacg acggtagcgt ccattggttg tttgcccgag caaaaatctt ccgcgacgcg
     6241 aatggggctc cagagagaat gctgggggtc aatgtcgaca tcacggagcg caaacaagct
     6301 gaggaggcgt tacgccgtag cgaggagcag ttggagctca tcagtgatac cgtacccgcg
     6361 ctgatctcgt acgtgaaccg cgaccgccaa tacgtcacat gcaatgaggc ctacaccaaa
     6421 tggtttggtc tatcccgaaa cgacatcgtc ggtaagacga tgcaagaagt cgtaggggca
     6481 gaggcgtggg gcgcaatggc ggcctatgtg gaggcggctc tccagggtca gacggtcgat
     6541 tatgagaccg aagcccgtta ccgatacggt ggtacaaagt ggattcacgc ggtctatacc
     6601 ccgcatcgag atgccgaagg ccatatcgcc gggttcatcg tcctggtgac tgacattagt
     6661 gccagaaagc gtacagaagc cgctttgcgt gaaagcgaga tgagatttcg aaccatcgct
     6721 gatagcgcgc ccgttttaat ttggatgaat ggattggatg gttgcgagtt cgtcaatcat
     6781 gcctatttgg aattcatcgg gagcagtcaa cagaaggatg tgctcaacta cgagtgggca
     6841 caatacattc acgacgacga tcgagagagt tacttacagt tgtattttga tgccatgcga
     6901 gaccaccgcc cctttgactc cgtgtgtcgc ttccgacgct ttgacgggca atatcgatgg
     6961 atgcggtcgg tcggaaggcc acgcctcgcg ccgaacgaca aactgatcgg atatgtgggg
     7021 gccaatgttg atatcactga catccaagag gcgcaggatc gtctccaacg atggagcgac
     7081 gaacttacga aggccgtgag cctcaagacg cacgagctgc tggaatctca agatcgactg
     7141 cggtcattgg ccgcagaagt gggtcgtgcc gaacaacggg aacgaaagcg aatcgccacg
     7201 gaattgcacg atcatctaca acagatgctg gtgttctgta agctcagact gggacagggc
     7261 aggcagcagg cggccgccat ccctgcctgt gccgaggtga ttaaacaagt tgatgatgtc
     7321 ttgagtgaag ccctgacgta ttcgcgaacg ttggtggccc agctgtgccc tccggttctg
     7381 caggagttcg gccttgccgc cgctcttcaa tggttgggcg agcagatgag ccaacgtaag
     7441 ttgcacgtct ctgtacacgt ggagcatata cccgtccctg aagagcaggc cgtgcttcta
     7501 ttccaatcgg tccgggagct gttgatgaat gtcgtgaagc atgctggaac cgcttacgcg
     7561 agtgtggaga tgcagaggga taaaaaagcc ctacgcatcg aggtgcgcga cagcggcagc
     7621 ggattcgatc ccgcggttgt tcaactatat gccggcgctc agccacctaa gttcggcctt
     7681 ttttccattc gcgagcggat gaaggccttt ggcggtatgt tggaggtgga aacgggtctt
     7741 ggaaaaggaa catccgctgt gctgttgctt ccacttgagg agaccaagaa cgagacgtcc
     7801 ggatcgcccg tctatgacca accggtggcg gtcggccagc tgaaaccggg tgcaagggag
     7861 atgccgcgac cgagtgagaa gcaccggctg acggttcttc tggtcgacga ccatgcgatg
     7921 atgcgagagg gtttgcgatc ggtattagag gcgcattcag acattcatat cgtcggtgag
     7981 gcgtctgatg gccaagaagc catcgctttg gtggatgaac tccgaccgtc actcgtcgtg
     8041 atggatatta atatgccccg actcaacggc atcgaggcta ctgcacgaat caaggcgcga
     8101 tacgctgaga tcaaggtgat cggtttgtcg gtgaatgcgg ggccgaataa caaggaggcc
     8161 atgctacgag ccggggccga tatgctctta accaaggaag ccgcggtgga ggagctttat
     8221 cgcagcattc aagaagtcat gcctcgcata taaaaacaac tttagatccc aagcactagt
     8281 gttctgacgc atatcaacgc ccccacaagg cggcgccaag cctagcttcc cttggccttg
     8341 ttttccaaca cctcgatgat tgaagagcag gccgacgctt ctagagactc gacagaagat
     8401 tgtgatgctg cctcgtcgac aggagacgca tgtaaacttc gaaacgtctt aacagcggtc
     8461 tgaacagcca gaaccagaaa ttgttgctca ttctcagcat agggaacttc tagagacaca
     8521 gtcatggggg acggcattca acctcctaat tcatacaatg caaggtgaag agtgcagacc
     8581 ttgggatgtc tgcctcgtaa gggtcactgg acgcgttccc ggctaaaagg gtttatgcct
     8641 accagtgaga aggaattgat cggggcggta catcatcgat agctctttag agatcgggta
     8701 agacttgaaa cggattacgc ttgagtagcc caaaaggaaa tacagtgaga ggcagtgttg
     8761 catctccggt ccgatggctc actcgtcaac atgaaagcat ggaaccttcg caccggccgc
     8821 atgaacatgt gctcacccag cccattttac atcagtcaaa gtgcgtccac tcatgatggg
     8881 gttaagacgg ttgactaaag catgagaagt cccgaagacc acgcttgcac gccaccgcat
     8941 tgctcttcga gccattcaag actgtactat gtagtcgccg tgcgacctct ttccgaggct
     9001 ccgtttgaaa tgtcccggga atgcttatgc aaacatggtt cgatcttttc gagaatttcc
     9061 ttgcagtaga acgctatgac ttctgcttca tcattcgaca gcgacctgtg cgtagctatg
     9121 gatgagacga atcgttcgca ggcatcagag aaactgtgga atgctttggc atgctgcatg
     9181 gtacctccat gagtagtggt agtacctcat cagccttcgg gcatttcggg acagctcacc
     9241 acgaggcacg agcgtaatgg cgagcaatct atggttaatc gcatgaaccg tctcctggga
     9301 tgttccctag ctactgcagg gacagaacga agagcttaat aagttaatcg cagtgtcaga
     9361 ttacgtatgt atgaagagcg acaggttggt tgctcgtgca atagaatgag cgtcgagaag
     9421 tctgcgctgc cgcgagaaaa acttaaacat ctcaaataag gagtcataac cttttagggg
     9481 gaaactggaa ccagtcgggg gcgaactggc tattggtgtg caacaaccgt ccagtcgttg
     9541 cacatcgcac caggccctcg
//
LOCUS       DASSCQ010000201         6351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1887015, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000201 DASSCQ010000000
VERSION     DASSCQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887015"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="gspG"
                     /locus_tag="VFH05_12870"
     CDS             <1..430
                     /gene="gspG"
                     /locus_tag="VFH05_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET6679161.1"
                     /translation="EIMVVVAILAILAALVVPRIMGRTDDAKRTAAKVQIRNIEGALQ
                     LYKLDNGVYPSTEQGLKALVEKPSVGVVPKKWKLGGYLPSIPEDPWGNPYKYLSPAPV
                     QQGQYGQIKADYEITSYATDGEAGGEGVNADITNWNMEKD"
     gene            435..1103
                     /locus_tag="VFH05_12875"
     CDS             435..1103
                     /locus_tag="VFH05_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679162.1"
                     /translation="MEPKARFELLRGEKSEAALGSASIVKSRRLIDASGFTLLEIVMV
                     LFLLTGMLSLVIPKVSIGQNLGSVGRKWVGSLKTLQDLSMTTQKTVRLYVDIDRGMYW
                     PMVLDGNEEKTPQDPIWAYPLSLPETVRFSDIQVGATRKETGRVDIFFYPNGRIDQAT
                     VHLTDSNNNVMGIFVEPVTALIRVTDHRIDPPKPLAMLERIRPLLQPITPGLRPASVM
                     GAPK"
     gene            1134..1577
                     /locus_tag="VFH05_12880"
     CDS             1134..1577
                     /locus_tag="VFH05_12880"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET6679163.1"
                     /translation="MPRQHVSRHSSAKADGFTLLEVLIAMAILAFALPVLLGLRNRDL
                     DFHARAADITAATMLAQEKLIEAELSPVYPIGETTGDFRNPPPGYQVPGDIANRAQRF
                     RWKRLITSTPLPSVREVKIQILWEQGAADEVLEASTYVFASTTGF"
     gene            1549..2169
                     /locus_tag="VFH05_12885"
     CDS             1549..2169
                     /locus_tag="VFH05_12885"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HET6679164.1"
                     /translation="MSLHQRPGFDEGFTLVEVLLATALVAVIAAMVFGSLHVSAAAID
                     GARNAAAREQLLRSTLRIMMEELTAAVANPLAPMVGVNATQDGQPADTVVFLTLSQFH
                     AGNAGQESEMVRIVYTRENDRLLRFIRRNLYGMTDETIDQSELAKLVKGFNLRYYDSM
                     ANAWLDEWDGRSKTAPPAAMLIELMLLEDNDEVRTFRQWVPIGMKS"
     gene            2169..3062
                     /gene="gspN"
                     /locus_tag="VFH05_12890"
     CDS             2169..3062
                     /gene="gspN"
                     /locus_tag="VFH05_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HET6679165.1"
                     /translation="MARLSWTPTIALKKEPVAWVLGGLATFAVFMFVTFPFGTLQARV
                     LSELVKATGGDIRAAEWSPGIPIAVEWRDLTWTKPGTSSIPIQVMRLNVGVMGLLMGQ
                     KTVEALIQFPGSGQAAKATGTVTASSWSFEGPVSLKARAQHIDLATVIKPYVTRGLLE
                     ADIAQRWENRGKEGIAFKGNGSWKAEIKELTLERLPVGTITVPSLTFTRVTATLSCHD
                     AICDVTDFKGDGPDGTITALGRLLLRQPLQSSGLELTVTVVPGAGWAQKAGTLPIPPL
                     PPGTPLTLKLAGSVANPKLTL"
     gene            3102..4676
                     /gene="pilM"
                     /locus_tag="VFH05_12895"
     CDS             3102..4676
                     /gene="pilM"
                     /locus_tag="VFH05_12895"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HET6679166.1"
                     /translation="MVAECVGLDLGQTAIKAVRFRRRLSGRESVEYFHLPLPYGKPVQ
                     AEPARLAGHLRNFLWQHGLYGSGDIVTALPCQDLFIRTLSFPFRDSAKLEQVVPFEVE
                     NLIPMALDEVAMGSVVLPAKVTGGDTQKPKGADVLITAAPKDKVAEHLKFLATADLKP
                     AAVGVDGMALYSVTKFLQEEGAHVPGDLAVIDVGATKTTLCLIHDGRPVVLRTVLWGG
                     NHLTHALAVRYACTFAEAERRKRAMGVQELEMWLEPLLRELRVSLHAYEGTAHQRLSH
                     CWVSGGGSKLRELSGHVAHELGLTPVGPRQGFGANCPRAFSIAFGLAIHPKIVRPRWK
                     TKAIGSDVALDLAATADGSAARSKESQQDRRLALWGGLILGVLVLVDLSVRVYVKEST
                     LTQLKQALQAQFAQSFGEGGPPGQELDVARSRVAQLDKSLAVIDRSRASVLVNLSDLA
                     KQLPAGIPLTIRELTVEGASVHLEGETNSFDSVEKIKQAFTSNQLFQAVSVTDTRVGA
                     VPSQVVFRLTYTVQRP"
     gene            4673..5221
                     /gene="gspM"
                     /locus_tag="VFH05_12900"
     CDS             4673..5221
                     /gene="gspM"
                     /locus_tag="VFH05_12900"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HET6679167.1"
                     /translation="MIATLQERWRHLASRERIIVASGGAVVLASLLFLLVVDPLFTRI
                     DKLERQAVRKSKERVELSALASEYAAKQARITKLEQRLPASEGQFSLLAFMEDAAASV
                     QIRDRIVGMQPQQATTQQGYEETAVDLRLDGVQLPQLLGLLVALEQAPYDVQVHHLQI
                     KPKYDNPINLDATLRIVSYAKS"
     gene            5310..6233
                     /gene="gspK"
                     /locus_tag="VFH05_12905"
     CDS             5310..6233
                     /gene="gspK"
                     /locus_tag="VFH05_12905"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HET6679168.1"
                     /translation="MALLLALLVLALLVALILEFDTDARREYRDAAAFRDNFKATVLT
                     RAAVQAARAVLQQDHLRDRQTGQAYDATTDLWAFPIEKYAIGDGLMTAQIEDERSKLN
                     LNDLAAGVDPNVKKTKMLRLKRLFNLLQINPDLVDAIADWVDADDIPEAAGAETVYYQ
                     SLRPSYRAANAPLQTLREIRLIKGMTPEIVDKLLRYVTVYPQEGESRVNINTADFLVL
                     QSLDPRISQTMAGEIIQGRPFKTVQDLDRIGSFAEIAKELRLLSLYDVKSNMFSAQMT
                     LTVNETTKSGAVVLQRDEATGNSMVRYFRFM"
BASE COUNT         1338 a         1783 c         1895 g         1335 t
ORIGIN      
        1 cgaaatcatg gtagtcgtgg cgattctcgc catcctcgcc gcactggtgg tgccacggat
       61 tatgggacga acggacgatg cgaagcggac ggctgcgaag gtccagatcc gtaatatcga
      121 aggtgcactc cagctgtaca aactcgacaa tggtgtctat ccgtcgaccg agcaaggtct
      181 caaggccttg gtcgagaaac cttcggttgg tgtcgtgccg aaaaaatgga agctcggtgg
      241 gtacctgccc agtattcccg aagatccctg ggggaatcca tacaagtatc taagcccggc
      301 gcctgttcag caggggcagt atgggcagat caaggccgat tatgaaatca cgtcgtacgc
      361 gacggacggc gaagccggcg gagaaggcgt caacgccgat atcacgaatt ggaatatgga
      421 gaaggattag gatcgtggag ccgaaagcca ggttcgagct gctcaggggt gagaaaagcg
      481 aggctgcgct cggttcggct tctatcgtga agtcccgcag actgatcgat gcaagcggtt
      541 tcacgctgct tgaaattgtc atggtgctgt tcttgttgac tgggatgctc agtctggtga
      601 ttcccaaagt atcgatcggg caaaatctcg gcagtgtcgg aagaaaatgg gtcggatctt
      661 tgaaaacgct tcaagacttg tcgatgacga cgcaaaagac ggtgcgattg tacgtcgaca
      721 tcgatcgcgg gatgtactgg cccatggtgc ttgatggcaa tgaagaaaaa actccacaag
      781 atccgatttg ggcctacccg ctcagtcttc cggagaccgt gcgattctca gacatccaag
      841 tgggtgcaac gagaaaggag accggccgtg tggatatctt tttctacccg aacggccgca
      901 tcgaccaggc tacggtgcat ctgaccgata gcaacaacaa tgtgatgggg atttttgtgg
      961 aaccggtgac ggccctgatc cgcgtcacgg atcaccgtat cgatccgccg aaaccgctgg
     1021 ccatgctcga acggatccgc cccttgctcc agccgatcac ccccggcctc aggccggcat
     1081 ctgtcatggg cgctccaaaa taacgcatta caggaacctg agtatggatg gtcatgccac
     1141 gtcagcatgt gagtagacat tcttccgcca aggcagacgg gttcacgctg ctcgaagtgc
     1201 tgatcgcgat ggcgattctg gcgtttgcgc tgcctgttct gctgggactg cgcaatcggg
     1261 atttagattt ccacgcgcgg gcggctgaca tcaccgccgc gacgatgttg gctcaggaaa
     1321 aactgatcga agccgaattg agtccggtct accccatcgg cgaaaccacc ggtgattttc
     1381 gaaacccgcc accggggtac caggtacctg gcgacatcgc caatcgtgcc caacggttcc
     1441 gctggaagcg cctcatcacg tcgacaccgt tgccatcggt tcgcgaagtg aaaattcaaa
     1501 ttctttggga gcaaggggct gccgatgaag tactcgaggc cagtacctat gtctttgcat
     1561 caacgaccgg gttttgacga gggcttcacc cttgtcgaag tgttattggc gacggcgctc
     1621 gtggcggtga tcgccgccat ggtcttcggc tcattgcatg tgagcgccgc ggcaattgat
     1681 ggcgcccgca acgcggctgc ccgggaacaa ctgttacgaa gcaccttgcg aatcatgatg
     1741 gaagaactca ctgcggcagt ggccaatccg cttgcaccca tggtgggagt caacgcgact
     1801 caggacggac agcctgccga tacggtggtc ttcctcacgt tgagtcaatt ccacgccgga
     1861 aatgctgggc aggaaagcga gatggtccga atcgtctata cgagggaaaa cgatcgcctg
     1921 ctccgcttca tcagacgcaa tctgtacggc atgacggacg agacgatcga tcaatccgag
     1981 ctggcgaaac tcgtgaaagg tttcaacctc cggtattacg actcaatggc caatgcctgg
     2041 ctggacgagt gggacggtcg ttcgaaaacc gctcccccag cggccatgct gattgaattg
     2101 atgttgttgg aggacaatga tgaggtgcgc accttccgtc aatgggtgcc gattggaatg
     2161 aaatcgtgat ggctcgcctg tcctggacgc cgacgatagc gttgaaaaaa gaacccgttg
     2221 cctgggtgtt gggcggactc gctacctttg ccgtcttcat gttcgtcacc ttccccttcg
     2281 gtaccctgca agcgagagtg ttgtcggagc tcgtcaaagc gaccggtgga gacatccgtg
     2341 cggccgagtg gtctcccggg attcccatcg ccgtggagtg gcgcgacctg acctggacca
     2401 agccggggac gagcagcatt ccgatccagg tgatgcggct taacgtcggt gtgatgggtc
     2461 tgctgatggg gcaaaaaacg gtcgaggcgc tcatccaatt tccagggagc ggacaggccg
     2521 cgaaggcgac ggggacggtc actgcctcct cctggtcgtt tgagggcccg gtttctctca
     2581 aggcccgcgc tcagcacatc gatctcgcga cggtcatcaa accgtatgtc accagggggc
     2641 tgttagaggc ggacatcgca cagcgatggg agaatcgcgg caaagaaggc attgcattca
     2701 aaggcaacgg ctcttggaag gcggagatca aagaactgac gcttgagcgg ctgcctgttg
     2761 ggacgatcac ggttccgtct ctgacgttca cccgggtgac ggcgaccttg agctgccatg
     2821 acgccatctg tgacgtgacc gatttcaagg gagacggtcc agatgggacc atcactgccc
     2881 tgggtcgtct cctgcttcgg caaccgttgc aatccagcgg attggagctc acggtgacgg
     2941 ttgtaccggg tgcaggctgg gcgcagaagg cgggaacatt gccgattcct ccccttccgc
     3001 cgggaacccc tctgactctc aaacttgcag gatcagtcgc aaatcccaag ctaaccctgt
     3061 agtatacagg cttaatctct tcaccggagc gcgcgtaggt catggttgca gagtgtgtcg
     3121 gactcgatct tgggcagacc gccattaagg ctgtccgatt ccggcggcgc ttgtccggac
     3181 gcgaatcggt cgagtatttc catctgccgc tgccttatgg gaagccggtt caagccgagc
     3241 cggcccgtct tgccggacac ctgcggaatt ttttgtggca acacggcttg tacggcagcg
     3301 gcgatatcgt gacggcgctc ccctgccagg atctcttcat tcgtaccctc tcgtttccgt
     3361 tccgtgactc ggcaaaactg gaacaagtcg ttccgttcga agtcgagaat ctcataccga
     3421 tggcgctcga tgaagtcgcc atgggcagcg tcgtgctgcc tgccaaagtc acagggggag
     3481 acacccagaa acccaagggc gccgatgtgc tcatcacggc agcacccaag gacaaggtgg
     3541 ccgagcatct caagttcctc gcgacggcgg atctcaaacc ggcagccgtc ggggtcgacg
     3601 gcatggcgct gtactcggtc acgaagtttc tgcaggaaga aggcgcgcac gtccctggcg
     3661 acctggccgt tatcgatgta ggtgcgacga aaaccacact atgcctcatt catgacggcc
     3721 gtcccgtggt gcttcggacg gtgctctggg gaggtaatca tctgacccat gcgttagccg
     3781 tccgctatgc ctgtaccttt gccgaggccg aacggcgcaa gcgcgcgatg ggggttcaag
     3841 agctggagat gtggctcgaa cctttattga gggagctgcg agtttcgctc cacgcgtacg
     3901 aaggaacggc gcatcagcgg ctctcgcact gttgggtctc cggcggtggc tccaaactgc
     3961 gcgagttgag cggtcacgtc gcacacgaat tgggattgac gcctgtcggt ccccggcaag
     4021 ggtttggagc caactgccca cgcgcgttct cgatcgcctt tggactggcc attcatccaa
     4081 aaatcgtgcg tccgcgctgg aagaccaagg ccatcggctc cgacgtggcg ctcgatcttg
     4141 ctgccaccgc ggacgggagt gcggcacgca gcaaagaatc acaacaggat cggcgtctcg
     4201 cgctgtgggg cggattgatt ctcggagtcc tggtcctcgt cgatctttcc gttcgtgtct
     4261 acgtcaagga gtcgacactg acgcaattga aacaggcgct gcaggcgcaa ttcgcgcaat
     4321 cgttcggcga aggcgggccg cctggccagg aactcgacgt ggcacgctcc cgcgtcgccc
     4381 agctcgacaa gagcttggcc gtcatcgacc ggagtcgtgc tagcgtgctg gtcaatctgt
     4441 cggatttggc gaagcagctt cctgccggca ttcctctcac gattcgcgag ttgacggttg
     4501 aaggagcttc cgttcacctg gaaggcgaaa caaactcatt cgattccgtt gaaaagatca
     4561 aacaagcctt cacctctaac cagctgtttc aggcggtctc ggtcaccgat acgcgggttg
     4621 gcgccgtccc gagccaggtt gtctttcgac ttacctacac ggtgcaacga ccatgatcgc
     4681 tacccttcag gaacgctggc ggcatctcgc ctcgcgagag cggattatcg tggcgagcgg
     4741 cggggccgtc gtgctggcga gtctcctgtt tttgctcgtc gtcgatccgc tctttacgag
     4801 gatcgataag cttgagcgtc aggcggtcag aaaatcgaag gaacgtgtcg aattgtcggc
     4861 gctggcatcc gagtatgccg caaaacaagc ccggatcacc aagttggagc aacggcttcc
     4921 ggccagcgag gggcaattct ctcttctggc ctttatggag gacgctgcgg cttctgtaca
     4981 gattcgggat cgcattgtcg ggatgcagcc gcagcaggcg acaacccagc aagggtacga
     5041 ggaaacggca gtggacctca ggttggatgg agtccaattg ccgcagctgt taggcctgtt
     5101 ggtcgcactg gagcaggcac cgtacgatgt ccaggttcac cacctccaga tcaagcccaa
     5161 atacgacaac ccgattaacc tggacgcgac gctccgcatc gtgtcctatg ccaaatcttg
     5221 accgttcggt tggtcgccgg cgatcccctg tgcagagcag acaggacgcc ggtgtccttt
     5281 gttccatcgc tagagatgac gaacgtggtg tggctctgct cctcgcactc ctcgtcctgg
     5341 ctctccttgt cgccctgatc ctcgaattcg acaccgatgc ccggcgcgag tatcgcgacg
     5401 ccgctgcctt ccgagacaat tttaaggcca ccgtgctcac ccgggctgcg gttcaggccg
     5461 cacgtgccgt gttgcaacag gatcatctgc gggataggca aacggggcag gcgtacgatg
     5521 caacgaccga cttgtgggct tttcccatcg aaaaatatgc gattggcgac ggcctgatga
     5581 cggctcagat tgaagatgag cggagcaaat taaatctgaa cgatttggcg gccggcgtcg
     5641 atcccaatgt caagaagacg aaaatgctga ggctaaagcg gctgtttaat ctgctgcaaa
     5701 tcaatcccga tctcgttgat gccattgccg attgggtcga tgccgacgac attcctgaag
     5761 cggccggcgc tgagaccgtc tattatcagt cgcttcgacc ttcttatcgg gcggccaacg
     5821 ctccgctgca aacgctccgt gaaatccggt tgatcaaggg catgacgccg gagattgtcg
     5881 ataagttgct tcgctacgtg acggtctatc cccaggaagg cgagagtcgc gtcaacatta
     5941 atacggcgga ctttttggtc ttgcaatcgc tcgatccacg catcagccag acgatggccg
     6001 gcgagatcat acaaggccgc ccgttcaaaa ccgttcaaga tctggatcgc atcggtagtt
     6061 tcgctgaaat cgcaaaagaa ctccgcctgc tcagcctcta cgatgtcaag agcaatatgt
     6121 tctcggcaca gatgaccttg acggtgaacg agacgaccaa atcgggcgcg gtcgtgcttc
     6181 agcgggacga ggcgaccggt aacagcatgg tccggtactt tcgttttatg tgagcgattg
     6241 acccttcggt gtcccattct ctgtgaaagt taacaattct gtaacagtgc cgtcatccgg
     6301 gcgacatccc gggtaggtat cctcctagca tcaatattag aggaggtcac c
//
LOCUS       DASSCQ010000202         8006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1505603, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000202 DASSCQ010000000
VERSION     DASSCQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505603"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFH05_12910"
     CDS             <1..450
                     /locus_tag="VFH05_12910"
                     /inference="COORDINATES: protein motif:HMM:NF019938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HET6679169.1"
                     /translation="SNGIRVVCLRTGMVLGQDGGALPRIALPFRFFLGGPVMPGTQWI
                     SWIHRDDLVGLIEWALATPAVSGPINGVAPSPVTMAEFCRLLGLVLRRPYWLPVPEFV
                     LNTALGEMGSMMTTGQRAVPAVAEAHGYPFRFREAQRALQAIFSSLR"
     gene            540..986
                     /locus_tag="VFH05_12915"
     CDS             540..986
                     /locus_tag="VFH05_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET6679170.1"
                     /translation="MKSAILFVTLSVLSGLLFWGGASVADQSTEPSLKQEGMQLPKGV
                     IGVSLDVSAERIGDPAILYVAMVNPEGPAHRAGLAHGDEIQAVDETPVAGKTYEQVVK
                     MIRGEPGTTVKLTVKGEKGAREVSVTRVAADRLPMGPKGSHGSQAR"
     gene            992..1522
                     /locus_tag="VFH05_12920"
     CDS             992..1522
                     /locus_tag="VFH05_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679171.1"
                     /translation="MRRTAIILSLCVAIDLVPAVLLSVEPPQPVCGAVALMTKLDRYL
                     AAGDYRRALELAQREVDETPSAASYIHLTYVYHAIDAYLAYLSQEESWTAVEHLYLNL
                     TYRDSEDLVDPPGGLARMAKEMIQTSVRQQSDVSSAMAARLDKTESDRLWVEQAGWRR
                     TNEKTWWQSVPSSWRG"
     gene            1532..1954
                     /locus_tag="VFH05_12925"
     CDS             1532..1954
                     /locus_tag="VFH05_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679172.1"
                     /translation="MKILSGGTDIKLLGGSFIIVGMVDAIIIVLFPDYALKLFGAAVT
                     GPASLLIKLHSPLVHLLIGYGFVFLRPWGWGLAVAYSGFGLVSETMNQWSFGVSLIRS
                     GFMMTIALFAGYLIWRRHRFADEQPGEKSAKSVSQGSH"
     gene            1954..3399
                     /locus_tag="VFH05_12930"
     CDS             1954..3399
                     /locus_tag="VFH05_12930"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET6679173.1"
                     /translation="MRGIIWFRRDLRLADQAALSEACKDCREVIPLFIFDEPLLRSRQ
                     FGSACVNFMLGCLRELASSLAASGLALQWRRGDPVEEVVRAAREWNADVVYWNRDYEP
                     DAIARDREVVQRLGQAGIAVRTFKDHVVFETEEVRGVTGEALQRYSAYRARWWTKWHA
                     TKPALRGLPPDLSRKFGAPIPAVSPIPGAEELGYETVRPWIESGERAAQARLRWFVDG
                     PIHRYVDGRNQPAMDGSSKLSPHFRFGTLSPLAAVHAALRVLASGGHVSRPDVLTWID
                     ELIWREFFQQVLASFPRVANGPFRQASVPPCRLAGLERDRLFQRWSEGRTGYPIVDAG
                     MRQLNQTGWMPNRVRMITASFLVKDLRIDWQSGERYFMNHLLDADVAANNGNWQWCAS
                     TGTDAMRGYRIFNPALQSKKFDPEGRYIREYVPELAQVMDRHIHEPHLMTDEEQARAG
                     CRIGVEYPEPIVDHKQAREDYLALGKAEAMK"
     gene            3396..4349
                     /locus_tag="VFH05_12935"
     CDS             3396..4349
                     /locus_tag="VFH05_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HET6679174.1"
                     /translation="MTGQMLRLGISRCLLGESVRFDGGHKHDHFLTDVLGRYVEWVPV
                     CPEVEAGLGTPREAMRLVGDPDRPQLITVKSGMDHTEAVERVSANRVQNLKRLSLSGY
                     VFKKDSPSCGIERVRLYNRSGMPSRQGVGIFARAFMNQFPLIPVEEEGRLCDPDLREN
                     FIERVFSYRRYQDLLVKSPSRQSVVQFHTMHKYLLLSHSHPHYEALGRLVGQAHRYHP
                     KELAMKYGELFMKALAVKATVRKHVNVLQHIVGFFKTRLTAQEKRELLGVIDDYHRGL
                     TPLIAPLTLIRHYVTIFDVSYIHAQVYLNPHPKELMLRNHV"
     gene            4431..4871
                     /locus_tag="VFH05_12940"
     CDS             4431..4871
                     /locus_tag="VFH05_12940"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HET6679175.1"
                     /translation="MTKLKRLEAQRRAQKLPPSQEETELDQTIRRWPRTVATDPYRAG
                     LEAVGAALRPLLDGALFALAYNEFCSPTVEESIEELIRQGATHITVATTMFTPGGAHS
                     EVEIPEILTGVRARHPEVAIHYAWPFDASTIAHFMNEQVRRFMS"
     gene            complement(5027..6265)
                     /locus_tag="VFH05_12945"
     CDS             complement(5027..6265)
                     /locus_tag="VFH05_12945"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HET6679176.1"
                     /translation="MYKTTEIDETIQTDQPGTGIHREEDVTLFDYWQVIYSRRKAIVI
                     FCTVMVLATLCVSLLMPKIFESTATLLPQLESNTGLGMGLGSLLSSSGAGSAAQSLGI
                     SLPGAPATPTDVFNAMLKSRIMADEVIHKFNLMEHYETKTMQDTRSALEGATRIVLSK
                     EKVIKVTVEDKDPQLAADIANFYVSNLDHLNQTLSVSKARENRKFIEQRVAETQTELV
                     KVENALKEFQTQNRTVAIEAQSKAMIEATAMIQAQIMAQEVQLQVMGSYLSPNNPEVA
                     RVQSSIHELRKQLQTMETGKSGKDRLPGDRLRPAITSVPTLALEYGRLARDLKVQETL
                     YALLISQYEQAKLTEARDTPTVQVLDPAIPAERKSRPKIALNLLIAGILSLLIGIFWA
                     FLREAIDRRKSRFSPLPSFS"
     gene            complement(6320..>8006)
                     /locus_tag="VFH05_12950"
     CDS             complement(6320..>8006)
                     /locus_tag="VFH05_12950"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HET6679177.1"
                     /translation="AIPVGGLTFSQAERAITNRLGAHLKRFEVHVAMARLRTIKVYVV
                     GEVVRPGAYEISSLATVSNALYAACGPARSGSLRQVRLVRENQAIGEVDFYQFLMAGD
                     RSKDARLQSGDVIVVPPLGPVAAVGGAVKRAAIYEIKSGTRLVDLLQLAGGTTPSANL
                     QRCQLLRLDQEKGRIMTDIDLAQIIDASGKKRPTSNEADVTLQDGDFLRVASLPTQVV
                     NVVTLAGAVKNPGPYEFRPGMAVRDILKADQLAVDAYVDKAEIVRTDPLTYETQVISF
                     SPKALFDNEKAGDIPLQRMDQVVVGTQMRPPSVVFVEGEVRRPGYFTLETGEKLSSVL
                     KRAGGFTTTAFPEGLVLVRESVRKRQQLELDRFLAAERQRLTAQSAAIAAGTVGMSPV
                     GGAAAGQAAEQQVLNLRLQQLEAVAARVELGRVVVNVRTIEELEGSEDNLMLEAKDRI
                     MIPQPPKTVSVIGSVKNPSTVVYRVGLNLEDYVQQTGGMTEDASKSEMYVVKANGSTE
                     GAYVRLKDMKPGDTIVIPQKIEAKTPQLSLWQSVASIIGSVALTAAGIAVIGR"
BASE COUNT         1653 a         2243 c         2344 g         1766 t
ORIGIN      
        1 tcgaatggga tccgagtcgt atgtctgagg acagggatgg ttctcggaca ggatggagga
       61 gccctgccgc gtatcgcact ccccttccgt ttcttccttg gtggccctgt gatgcccggc
      121 actcaatgga tctcgtggat ccatcgggac gatctggttg gtttgatcga atgggccttg
      181 gcaacgcctg ccgtgagcgg tccgatcaac ggcgtcgctc cctcgcctgt gaccatggct
      241 gaattctgcc ggctgctggg tctcgttctc agaaggcctt attggcttcc cgttcctgaa
      301 tttgtgttga atacggcgct cggagagatg ggatcaatga tgaccacggg gcagagggcc
      361 gtgccggcgg tcgcggaagc tcacggctac ccattccggt ttcgggaagc tcagcgagca
      421 ttgcaggcca tcttttcttc attgcggtga ccatgaaaac atcgtccccc tatttcacac
      481 actctagcgg ccggtatcct caagaggagg aaaaactctt gaatcgtaag gagggaacca
      541 tgaaatccgc aatactattc gtgacgttga gcgtgctgtc aggactgttg ttctggggag
      601 gagcctcggt cgccgaccag tctacggagc cctctttgaa acaagaaggc atgcagcttc
      661 ctaaaggagt gatcggcgtg tcgctcgatg tcagtgctga acgcatcggt gacccggcca
      721 ttctatatgt cgctatggtg aatccggaag gaccggctca ccgggcaggc ctggcgcacg
      781 gcgatgagat ccaagcggtt gatgaaacgc cggtcgcggg caagacctac gagcaggtcg
      841 tcaaaatgat tcgtggagag ccgggcacga cagtcaagct cacagtcaaa ggtgagaagg
      901 gcgctcgtga agtgtcggtc actcgggtcg ctgcagaccg tcttccgatg ggaccgaagg
      961 gctcgcacgg cagccaggca aggtaggagt catgcgacgc acggcgatca tcttatccct
     1021 ctgcgtggca atagatcttg tgccggccgt tctgttgtcg gtagaaccgc cacagccggt
     1081 gtgcggcgcg gtcgccttga tgacgaagct cgatcgctat ctggcggcgg gtgactaccg
     1141 tcgcgcactc gaactcgctc agcgagaagt cgacgagacc ccttcggcgg ccagctatat
     1201 ccatctgact tacgtgtatc acgcgatcga cgcgtatctg gcgtaccttt cgcaagaaga
     1261 gagctggacc gccgttgaac acttgtactt gaacctaacg taccgcgaca gcgaggatct
     1321 ggttgaccca ccgggcggcc tggcacggat ggcgaaggag atgattcaga cgagtgtgcg
     1381 tcaacagtcg gacgttagct ccgcgatggc ggcacgcctc gacaagacgg aatcagaccg
     1441 cttgtgggtg gagcaggcag ggtggcgcag gacgaacgag aagacctggt ggcagagcgt
     1501 gccaagttcc tggcgaggat aagaggtacc catgaagatc ctatccggag ggacggacat
     1561 caagttgctt ggaggatcat tcatcatcgt gggaatggtg gatgccatca tcatcgtcct
     1621 atttccggat tatgcgctga agctgtttgg cgcggcggta accggtcccg cgtctctgtt
     1681 gatcaagctg cattcccccc tggtgcatct gctgatcgga tatggcttcg tttttctgag
     1741 gccctgggga tgggggttgg ccgtggccta ctccggcttt ggtctggtga gcgagactat
     1801 gaaccagtgg tccttcgggg tgagtctcat acggagcgga ttcatgatga cgatcgcctt
     1861 gtttgcaggc tatctgatct ggcgtcgcca ccggttcgcc gacgagcagc caggcgagaa
     1921 gagcgcaaaa tctgtgtcac aggggtcaca ctgatgagag gcatcatttg gttcagacgg
     1981 gacctgcgcc tcgccgacca agcagcgctg agcgaggcct gcaaagactg tcgcgaagtc
     2041 attcccctat tcatcttcga cgaaccgctg ctgcgctccc gtcaattcgg ttccgcctgc
     2101 gtcaatttta tgctggggtg cctcagggaa ctggcttcgt cgctggctgc cagcggcctg
     2161 gcgctgcagt ggcgtcgagg ggatccggtc gaagaagtgg ttcgcgccgc acgtgagtgg
     2221 aacgccgacg tggtctattg gaaccgcgac tacgagcccg atgccattgc gcgtgaccgg
     2281 gaggtcgtgc agcggctcgg ccaggcggga attgccgtcc gcacgttcaa agaccacgtc
     2341 gtgttcgaga cggaggaagt gcgcggcgtg acgggtgaag cgttgcaacg atacagtgcc
     2401 taccgggccc gttggtggac caagtggcat gccaccaaac cggctttacg gggtctccct
     2461 cccgacctgt cacgaaaatt cggcgcaccg attcccgcgg tcagtccgat tcccggcgcg
     2521 gaagaactgg gttatgaaac ggtcaggccg tggattgaat ccggagagcg ggcagcgcag
     2581 gcgcgattgc gctggttcgt cgacgggccg atacaccgct acgtcgacgg ccgcaatcaa
     2641 ccggcgatgg acggaagctc caagctgtcc ccccatttcc ggtttggcac gctgtcgcct
     2701 ctcgcggcgg tgcatgcggc cttaagggtt ctggccagcg gtggccacgt gtcacgtccc
     2761 gacgtgttga cctggatcga cgaactgatt tggcgggagt tctttcagca ggtcttggcg
     2821 tcgttcccgc gcgtcgcaaa tggtccgttc cgacaggcgt ctgtgcctcc ttgccgcctg
     2881 gcagggctgg aacgtgaccg attgttccaa cgctggagcg aagggcgaac gggatatcct
     2941 atcgtggatg cggggatgcg gcagctgaat cagacaggct ggatgcccaa ccgcgtgcgc
     3001 atgatcacgg cttcttttct cgtaaaagac ctccggatcg actggcagag cggcgagcgg
     3061 tattttatga accatctgct ggacgccgac gtggcggcca acaatggaaa ctggcagtgg
     3121 tgtgcttcca ccggaacgga tgcgatgaga ggataccgca tattcaatcc ggctctccag
     3181 agcaagaagt tcgatccgga aggccgatat atccgagagt atgttccaga actggcacag
     3241 gtcatggacc ggcatattca cgagccacat ctgatgaccg atgaggaaca ggcacgtgcc
     3301 ggctgccgga tcggtgtcga gtatcccgag ccgatcgtgg atcacaagca ggcccgcgaa
     3361 gactatctcg cgctgggaaa agctgaggcc atgaaatgac cggccaaatg cttcgcctcg
     3421 gaatcagccg ctgtttgctt ggagaatcgg tccgctttga cggaggtcac aagcacgacc
     3481 atttcctcac cgacgtgctc ggccgttacg tcgaatgggt gccagtttgt cccgaagtag
     3541 aagctggcct gggaactccc agagaggcca tgcggctggt cggcgatcct gaccgtccgc
     3601 aattgatcac ggtcaagagc gggatggatc ataccgaagc ggtagaacga gtgtcggcga
     3661 accgtgttca gaatttgaag aggttgagtc tttccggcta tgtcttcaag aaggattcac
     3721 cgagctgcgg tatcgaacgt gtgcggctgt acaaccgatc tggcatgccg agccgccagg
     3781 gcgtcggcat cttcgcgcgg gccttcatga atcagttccc cttgattccc gttgaggaag
     3841 agggacgact gtgcgatccc gacttgcgtg aaaatttcat cgagcgggtg ttctcctacc
     3901 ggcgctatca ggatcttctt gtgaaaagcc cttccaggca gtctgtcgtg cagttccaca
     3961 cgatgcacaa atatttgctc ctctcccata gccatcctca ctatgaggcg ctgggaaggc
     4021 ttgtcggtca agcccaccgc tatcatccaa aagagctggc catgaaatat ggagagctct
     4081 tcatgaaggc gctggccgtg aaagccacgg tgcggaaaca cgtaaatgtg cttcaacaca
     4141 ttgttggatt ctttaaaact cggctgacgg cccaggaaaa gcgcgagctc ctcggcgtca
     4201 tcgacgacta ccaccgaggg ctcacaccgc tcattgcgcc gctgacactt atcaggcatt
     4261 acgtgacgat cttcgatgtg agctacattc acgcgcaggt ctacctcaac ccgcatccga
     4321 aggaattgat gttacgcaac catgtctagg gggacccaat ggtgacaggt aaaacaggcc
     4381 ttgtgctcgt cggtcacggg ggaatcccta aggactgtcc gcaggagctc gtgacaaagt
     4441 tgaagcggct tgaagcgcag cgccgcgccc agaagctgcc gccgtctcag gaggaaactg
     4501 aactcgacca aacgatccga cggtggccaa ggactgtcgc caccgatccc taccgcgccg
     4561 gattggaagc cgtcggtgcc gcattgcgac cgctgcttga tggtgctctc tttgcattgg
     4621 cttacaatga attctgctct ccgacggtgg aagaatcgat cgaagagctt atcagacaag
     4681 gtgcgacaca catcacggtg gccaccacga tgttcacacc cggcggagcc cactctgagg
     4741 tcgagattcc ggaaatcctt actggtgtca gagcgagaca tccggaagtc gcaatccact
     4801 acgcatggcc gttcgacgcc tcgacgattg ctcatttcat gaacgagcaa gtacgacggt
     4861 tcatgtcatg atagttggcc acggaccgca gcgccgttag gacgcttcgg tatgagagaa
     4921 agaatggcgt cgatccgagc ccggtggatc ggacttatca caaatgattg tggaaaaggt
     4981 gaagggaaag gtgcggtggg agctcagtgg aagaacgagt ctgtgatcag gagaaggagg
     5041 gaagtggtga gaatcgggac ttccggcggt ctatcgcttc gcgaaggaat gcccagaaga
     5101 ttccaattag caggctaagt atccctgcga tcagaagatt caacgcgatc ttgggtcggc
     5161 tctttcgctc ggcaggaata gcggggtcga gcacctgcac ggtaggagta tcccgcgctt
     5221 ccgtcagctt ggcctgctca tattgggaga ttaataaggc atacagggtc tcttgaactt
     5281 tcaagtctcg ggccaaccga ccatactcta aggcgagcgt cggtacactc gtgatggctg
     5341 gtcgtaatcg atctcctggc agacgatcct ttccgctctt gcctgtctcc atagtttgca
     5401 gttgctttcg taattcatgg atgctggatt ggactcgggc cacctcaggg ttgttcggag
     5461 acagatagga tcccatcacc tgcagctgaa cctcctgcgc cataatctgg gcttgaatca
     5521 tcgcggtggc ttcgatcatc gcttttgatt gtgcctctat ggccaccgtt ctattctgtg
     5581 tttgaaactc cttgagcgcg ttctcgactt tcaccaattc cgtttgagtt tcggctactc
     5641 gctgttcgat gaacttccta ttttctctcg ctttactgac actcaacgtt tggttcagat
     5701 ggtcgagatt cgacacatag aagttcgcga tatcggctgc cagttgagga tctttgtctt
     5761 cgaccgtaac cttgatgacc ttttctttgc tgagaacgat acgggttgct ccttccaagg
     5821 cggaacgcgt gtcctgcatg gtcttcgtct cgtaatgttc catcaggtta aacttgtgga
     5881 tgacttcatc cgccatgatt cgcgatttga gcatggcgtt aaacacatcc gtcggtgtcg
     5941 ccggtgcgcc ggggagcgaa attcccaagc tctgagcggc gcttccggca ccgctggaag
     6001 acagcagcga acccagcccc attcccaacc cggtgttaga ctccaattgt ggaagcaatg
     6061 tcgcggtcga ttcgaagatt ttgggcatca acaggctcac acataaagtc gcaagaacca
     6121 tcacggtgca aaagatcacg atggccttac gcctcgagta aataacctgc caataatcaa
     6181 acagggtgac gtcctcttcg cggtggatgc ccgtcccggg ctggtcagtc tggattgttt
     6241 cgtctatctc ggtggttttg tacatcggtc cgtccagccg attggttgtt ccggtgagat
     6301 cagatatccg cgggcgatac tatcgcccga ttaccgcaat tccggcagcc gtcagtgcta
     6361 cgctcccgat gatactcgcg acgctctgcc ataatgacag ttgcggcgtt ttggcttcga
     6421 tcttttgtgg aatgacgatg gtatcgcccg gcttcatgtc tttaagacgg acataggcac
     6481 cctccgtcga gccgttggct ttcaccacgt acatttcgct cttactcgca tcttccgtca
     6541 tgccaccggt ttgttggacg taatcctcca ggttcaaacc aactcgatag acgaccgtgc
     6601 tcgggttctt gacggacccg atcacactga cagtcttcgg cggttgggga atcatgatgc
     6661 gatccttggc ctccagcatg aggttgtctt ctgatccttc caattcttcg atcgtccgga
     6721 cattgaccac gacgcggccc aattccacac gagcagccac cgcttcaagc tgctgaaggc
     6781 gcagattgag cacctgctgt tctgccgcct gcccagccgc cgcgccgccg acgggtgaca
     6841 ttcccactgt gcccgcagca atggcagccg attgggccgt gagccgttgc ctttctgccg
     6901 caagaaatcg atcaagttcg agctgttgac gcttccgcac cgactcacgc acgagcacaa
     6961 gcccttccgg gaatgcggtg gtcgtgaagc cgcctgcccg cttcaagaca gagctgagct
     7021 tctcacccgt ctcgagcgtg aaatagcccg gtcgcctcac ttccccttcc acaaacacca
     7081 cgctgggtgg acgcatctgg gttccgacca cgacttgatc catccgttgc agtggaatgt
     7141 cccctgcttt ttcgttatcg aacagcgcct ttggactgaa tgagatgact tgggtttcgt
     7201 atgtgagtgg atccgtgcgg acgatttcag ccttgtctac ataggcatcg accgcgagct
     7261 gatcggcttt gagaatatcg cgcacagcca tgcctggacg gaattcgtaa ggtcctggat
     7321 ttttcaccgc accagccagg gtcaccacat tcaccacttg cgtaggcagc gaggcgacac
     7381 gaagaaaatc cccgtcttgt agtgtcacat ccgcctcatt actggtcgga cgttttttcc
     7441 cgctcgcatc gatgatttgg gcaaggtcga tatcggtcat gatccggccc ttctcctgat
     7501 caagccggag taactggcag cgctgaaggt tagccgaagg agtggtacca ccggccaact
     7561 gaaggagatc taccaatcgc gttcccgact tgatctcata aatcgcggcc cgcttcaccg
     7621 ctccgcccac tgcggcaact ggcccgagcg gtggaaccac gatgacatcg ccggactgca
     7681 atctggcatc cttactgcgg tcgccggcca tgaggaattg ataaaagtcc acctccccta
     7741 tggcttggtt ctcccgaacc aaacgaacct ggcgcaatga cccggatctc gcgggaccgc
     7801 aggctgcata cagcgcgttg gaaacagtcg ccagggaact gatctcatag gccccgggtc
     7861 gtaccacttc tccgacgacg tacaccttga tcgtgcgcag acgggccatg gccacatgga
     7921 cttcgaatcg ttttagatga gcgccaagcc gattcgtaat ggcccgctct gcctgggaaa
     7981 aggtaaggcc accgactgga atggcc
//
LOCUS       DASSCQ010000203         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_72429, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000203 DASSCQ010000000
VERSION     DASSCQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72429"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..2201
                     /gene="asnB"
                     /locus_tag="VFH05_12955"
     CDS             261..2201
                     /gene="asnB"
                     /locus_tag="VFH05_12955"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6679178.1"
                     /translation="MCGIAGIMSVNGRPVLEDELRAMCAAMIARGPDDEGVYLDRGIG
                     LAMRRLSIIDLHTGHQPVSNEDGSIWVVLNGEIYNFQELREELSKRGHTFSTATDTEV
                     IVHLYEEFGDRCVDRLRGMFAFALWDERRCRLLIARDRLGIKPLYYAMVQGRLLFASE
                     LKAILQLPEVDRTVNVTALDHLLAYKTTPERHSIVSAVRKLEPGHCLTLTGSGEPRVE
                     RYWSLRFEPETGRTEDDVAEELRQRLMESVRLHMVSDVPVGAFLSGGIDSSAVVAAMS
                     AVGSGRVKTFSIGFHDEEFNELPFARMVANRLGTDHHELILEPNVVELLTDLAWDLDE
                     PFGDASAIPTYMVSKMAAQHVKVVLSGDGGDELFAGYTEYAVERRERRARFLPGIARQ
                     ALGIISDAMPDGVRGKNWLHHFSLPDAERYLDANTMFKRADRCRLLTKETQAQLLNGQ
                     TERSRLDRLTHGVGHWLSTLQSMDLDHYLPLDILTKVDRMSMAHSLEARVPLLDHRFV
                     EFAATIPPNWKMRRGRTKHIFVKALRGWLPDEILDRPKQGFAVPLDRWFRGPLRPLFR
                     EILLSDTCRRRGIFDEQFLRRLFARQEQGRQLDPHIWTLMSFELWCRRFLDGSRTPCV
                     RPASHSTLIAPSRVRRPLAASS"
     gene            2224..3327
                     /locus_tag="VFH05_12960"
     CDS             2224..3327
                     /locus_tag="VFH05_12960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6679179.1"
                     /translation="MIKTQRPPNIAMVAPTFGSAGGQAVQAKVLADHLRRDRYIVDVV
                     PINPPFPRGARWLKRLRYVRTVAHEGLYLPTLRKLRRADVVHVTAASYWSFLLAPLPA
                     IVAAHRWGKPILLHYSSGEADDHLANWGSLVHPWLKMVDAIVVSSVFLRDVFARHGYE
                     AQVIRNVIDTEQFRYRQRDPLLPEFLSVRNFEPHHGVENTLVAFALIQTAYPHASLTM
                     VGQGSQEAELKHLGQALALRNVRFLGPVEPASMPGLYDAHSIFLNSSFVDNQPLSVLE
                     AMASGMPVVSTGVGDIPNMIEDGESGTLVPAADPSAMAKAARVLLEHPARARLMAQRA
                     KESLVHYDWSTVGPAWAETYRRLATRFSEREVA"
BASE COUNT          636 a          970 c         1046 g          732 t
ORIGIN      
        1 tgaacgtgat actgattttc atcggaaggc tcatgcgggc atcgcgcaaa ttgaatgcgg
       61 ggaagccggt ggcccgtgaa gtcgtcagag ccagagcggc gtgagtggag cctgtgttca
      121 tcatcggcgg attccttctc attgcgtgta ggtgattgcg gcgggtgggt cacgcagtcc
      181 ggcgatgcaa tctcacgtgg acggcgtgct catcatgctt gagccccgtc gaattagcac
      241 cttgcgggag gacgattggt atgtgcggta ttgccggcat catgagtgtg aacggtcgtc
      301 cggtcctcga ggacgagctg cgggccatgt gcgcagcgat gattgctcgt ggaccagacg
      361 acgagggggt gtatctcgac cggggtatcg gtcttgcgat gcgccgcctg agcattatcg
      421 acctgcacac ggggcatcag cccgtctcca acgaggatgg atcgatctgg gtggtattga
      481 atggggaaat ctataacttt caggaactgc gggaggaatt gagcaagcgc ggccatacgt
      541 tttcgaccgc cactgacacc gaagtcatcg tgcatctgta cgaagagttc ggagatcgat
      601 gcgttgaccg tctccgtggg atgttcgcct tcgcgctgtg ggacgaacgc cgatgccggc
      661 tgctgattgc ccgcgaccgg ctgggcatca agccgttgta ttacgcaatg gttcaaggcc
      721 ggttgctgtt cgcgtctgaa ctgaaagcca tcttgcagct gcccgaggtg gaccggaccg
      781 tgaatgtcac cgcgctcgat catctgctgg cgtacaaaac gacgccggag cggcacagca
      841 tcgtgtcagc cgttcggaag ctggagcccg gccattgcct caccctcact gggtcaggcg
      901 aaccacgtgt cgagcgctat tggtcgttgc gctttgaacc ggagacggga cggacggaag
      961 acgacgtcgc agaggaattg cggcagcgtc tcatggaatc ggtcaggctg cacatggtga
     1021 gcgatgtgcc ggtgggcgcc tttttgagcg gcggcatcga ttccagcgca gtcgtcgccg
     1081 cgatgtcggc cgtcgggtcc gggagggtca agacgttttc cattggattt catgacgagg
     1141 agttcaacga gcttccgttc gcccggatgg ttgccaaccg gctgggcacc gatcatcacg
     1201 aattgattct ggagccgaac gtcgtggaac ttctcacgga tcttgcctgg gatctcgatg
     1261 agccgttcgg ggatgcatcc gcgattccca cctatatggt ctctaaaatg gctgcgcagc
     1321 atgtgaaggt ggtgctgtcc ggtgatggag gcgatgaatt gttcgccggg tacacggagt
     1381 atgccgttga gcgccgggaa cgtcgggcac gatttcttcc tgggattgcc cgacaggcac
     1441 ttgggattat ttctgacgcc atgccggacg gtgtcagagg gaaaaactgg ctgcatcatt
     1501 tctccctccc cgacgcggaa cgatacctgg atgccaatac gatgttcaag cgagccgacc
     1561 ggtgccgcct gttgacgaaa gagacgcagg ctcagctgct gaatggtcaa accgaacggt
     1621 cacgtctcga ccggctcaca cacggcgtag ggcactggtt gtctaccctt caatcgatgg
     1681 atctggacca ttatctcccc ctcgacatcc tcacaaaagt cgatcggatg agcatggcgc
     1741 attccctgga ggcgcgcgtc ccattgctgg accaccgctt tgtggagttc gcggccacga
     1801 ttccacccaa ttggaaaatg cgtcggggga ggaccaaaca tatcttcgtc aaggccttac
     1861 gcgggtggct tcccgacgaa attctcgatc gtccgaaaca aggcttcgct gtaccgctcg
     1921 accggtggtt tcgtggcccg cttcgtccgc tgttccggga gattctgttg tctgacacct
     1981 gtcgtaggcg cgggatcttc gacgagcaat tcctccgtcg attattcgcc cggcaggaac
     2041 aggggcgcca attggatcct cacatctgga cgctcatgtc gtttgaactc tggtgccgca
     2101 ggttcctgga tgggtcacgc acgccctgcg ttcggcccgc ctcgcattcg accctgatcg
     2161 ccccctcacg tgtgcgtagg ccactcgccg cctcgtcgta gtccgactca cgaaacagaa
     2221 gccatgatca agacgcagcg ccctccgaac atcgccatgg ttgcgcccac tttcggcagt
     2281 gccggtggac aagccgttca agccaaggtg ctggcggatc atttacgacg ggatcgatat
     2341 atcgtcgacg tggtgccgat caacccgccg tttccgcgcg gagcgcgctg gctcaagcga
     2401 ctccggtacg tccgaacggt ggcacacgaa gggctctatc tgcccaccct gcgaaagctg
     2461 cggcgtgccg atgtcgtgca tgtcacggcc gcatcctatt ggtcgtttct cctggcgcct
     2521 ctccccgcaa tcgttgcagc gcatcggtgg gggaaaccga tcctcttaca ctattccagc
     2581 ggtgaagcgg acgatcacct tgccaattgg ggatctctcg tgcatccgtg gttgaagatg
     2641 gtcgacgcca tcgtcgtgtc ttcggtgttc ctgcgcgacg tgtttgcccg tcatggctac
     2701 gaggcgcagg tcattcgcaa tgtgatcgat acggaacagt ttcggtatcg gcagcgcgac
     2761 cccctgctgc cggaattctt gtccgtacgg aattttgaac ctcatcatgg ggtcgagaat
     2821 acgctcgtgg cttttgccct gatccagacc gcctatcctc atgccagctt gacgatggtg
     2881 ggccaaggtt cgcaggaagc cgaactcaaa catctcggtc aagcactcgc tcttcgaaac
     2941 gtgcggttcc tcgggcctgt ggaaccggca tcaatgccgg gcttgtacga cgcccattcg
     3001 atattcctca attcttcgtt cgtggacaac caaccgttgt cggtactgga agccatggca
     3061 tcgggtatgc ccgtggtgag caccggagtc ggcgacattc ccaacatgat tgaggacggt
     3121 gagtccggga cgctggtccc cgccgccgat ccatccgcga tggcaaaggc tgcgagagtg
     3181 ctgctggagc acccggctcg cgcacggctc atggcgcagc gcgcgaagga gtcccttgtg
     3241 cattacgatt ggtccaccgt ggggccggcc tgggccgaga cgtatcgtcg tctcgcaaca
     3301 agattctccg aacgggaggt cgcctgacaa tgtgcgccat agcaggaatt ctgaaattcg
     3361 atcaccggga gcgggcagag cgat
//
LOCUS       DASSCQ010000204         7707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_512809, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000204 DASSCQ010000000
VERSION     DASSCQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512809"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1095
                     /locus_tag="VFH05_12965"
     CDS             247..1095
                     /locus_tag="VFH05_12965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6679180.1"
                     /translation="MQKLIAGSGLASRRKAEGLIAAGRVMVNGKIVTELGTRVNPERD
                     HVKVDGKHLSAAQPFVYLMLNKPRNVVSTLDDPGGRTTVKDYLRGVSVRVFPVGRLDF
                     DSEGLMLLTNNGDLAQALLHPRYHVPKTYLIKVKGVLTDEDIAQLEQGVRLEDGMTSP
                     AQVKKVRKVEANSWLEITIREGRKHQVKRMLESVGHPVIKLMRIRMGPLSLGHLEPGE
                     FRFLTDREANALRQLVDDRIALVEGEGEPVRPSRRQPKRSGWARSSKTKSPRGRAAWS
                     SMRKRV"
     gene            1097..2860
                     /gene="aspS"
                     /locus_tag="VFH05_12970"
     CDS             1097..2860
                     /gene="aspS"
                     /locus_tag="VFH05_12970"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET6679181.1"
                     /translation="MKSRTHRCGELNRSQVGQTVVLNGWVQRRRDHGMVLFIDLRDRS
                     GITQVVFNAERNAAVHKAAHTLRSECVVSVTGTVAARPGESRNPNLSTGDIEVFADAV
                     EILNEAKTPPFVIEDDAEVTEAIRLKYRYLDLRRPRMQRLLSLRHAIMQGTRGFLNAE
                     GFLEVETPILTKSTPEGARDYLVPSRVNPGQFFALPQSPQLFKQVLMVSGVDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLELSFVDRGDVMSLTERMIATIFREAGGVQLPTPFPRM
                     SYAEAMGKYGTDKPDLRFDMPLYDVTAFAGTSEFKVFREAAGKGKLVKALIVKGGSSI
                     ARSRIDALGETARSFGAKGLAWLKVASDGQLDSVIAKFLDAKAFAAALPEAKPGDLVL
                     FGADTPAIVHDVLGRIRLLLGEELGVIDKTAWKPLWVTEFPLLDYSQEEKRYVFMHNP
                     FAAPLDEDLALLDSEPLKVRAKAYDMVLNGNEIGGGSIRNHRSDVQLRILDLLGIGKE
                     QAQAKFGFLLDALDYGAPPHGGIAFGLDRLIMLLGGAESIRDVIAFPKTQRAQCPLTD
                     APSPVGADQLKELRIKLDLLE"
     gene            2881..4044
                     /gene="aroC"
                     /locus_tag="VFH05_12975"
     CDS             2881..4044
                     /gene="aroC"
                     /locus_tag="VFH05_12975"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET6679182.1"
                     /translation="MAGNTFGHIFTVTSFGESHGPAIGCVVDGCPPGLALSAQDIQQD
                     LDRRKPGTSRHVTQRQEADTVEILSGVFEGRTTGTPVALLIRNEDARSKDYGSLIDTF
                     RPGHADYTYWQKYGIRDHRGGGRASARETAVRVAAAAIARKWLRETYGVLIHGYLSQL
                     GPHQVPFKTWEAVTGNPFFAPDADVVATLEAFMDELRKSGDSVGARITTVAEHVPVGW
                     GAPVYAKLDAELAAAMMSINAVKAVEIGSGFASVMQRGSEHGDELTPEGFVTNHAGGI
                     LGGISTGQDIVVTIGIKPTSSIRISRRSIDKQGNPVTVETNGRHDPCVGIRATPIAEA
                     MMALVLMDHALLHRAQNAEVKTATPKIGGSVAASPSGRRGKPIAKVNPEPHEA"
     gene            4134..4937
                     /locus_tag="VFH05_12980"
     CDS             4134..4937
                     /locus_tag="VFH05_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6679183.1"
                     /translation="MTAPGSYDGRILDIKHATIYRGDTCVFEDLSFVLEEDQHTAVLG
                     PNGAGKSTILKLLAGEVHAVPNDVTSIRLFGEEQWNVWNVRRRLGMVSHDLQHQYMEQ
                     VTGLKVVLSGYYASIGTYGQQEFSYAHIARAHSLMEDLGIGFLTDRRYAAMSTGEQRR
                     CLLARALVHGPSVLVLDEPTSGLDLTATFHYLDLVRGYMRKGKTILLVTHHIHEIPPE
                     IRRVVLLKKGKVIQDGEKKAVLSEKHLSNLFDCRIALACANGWYQALPG"
     gene            complement(5030..6055)
                     /locus_tag="VFH05_12985"
     CDS             complement(5030..6055)
                     /locus_tag="VFH05_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6679184.1"
                     /translation="MCRGSYNRPAFLRSILTLLACLLMASHASAGDETRSGALERAKR
                     VTLGILEDTQDPRAPEKPGKIKIRGTGFHLHDGYIVTARHAVEKSSPSGQVVPKVIHV
                     LTTDLHEVAAQIVGESAYLDVVVYRLNQQDRPVLPVEAPFAVETPPVGSEVFTVGYPM
                     GWGPTMAFGRIGNANTFLQTVETRLIQADLSACSGNSGGALFNVEGEVVGILHAVIQT
                     DKDESHVYCSQMAFAIPGTLAARIVSAAIEGKPLAFSKLGIHMTAVKDGTKWRIAVKD
                     VAEPAKSAGIQKHDILLAIEDTEILDAAHLKNYLIERTAPGQRVAVKVRRAEADLTFT
                     VTLTGGG"
     gene            6129..6419
                     /locus_tag="VFH05_12990"
     CDS             6129..6419
                     /locus_tag="VFH05_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679185.1"
                     /translation="MRATLLTLCLAVALTPSPLLWAGEASTGSETSLADKIGDTAKKI
                     GKKIEEGVTKTAKKIEEKRIPEKVERKLKKAVDKTAEGFGKAEKKLKQKLAD"
     gene            complement(6439..6888)
                     /locus_tag="VFH05_12995"
     CDS             complement(6439..6888)
                     /locus_tag="VFH05_12995"
                     /inference="COORDINATES: protein motif:HMM:NF016798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HET6679186.1"
                     /translation="MVEEDACSAAPSPAPSGWEAMLADPPWAGRSFLAVSVATIGGLA
                     SWIGNYTSSTVAQLGGSYLGGYLIGWAFRRFLRMAAIMVGIGLALIGAMKTTGWVDVD
                     WNAVEAQVSQTVQWLHGEAQGLKNLLAGFLPSAGAAGAGSFFGFRKK"
     gene            complement(6907..>7707)
                     /locus_tag="VFH05_13000"
     CDS             complement(6907..>7707)
                     /locus_tag="VFH05_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET6679187.1"
                     /translation="GGVGGLGLGGIVLVLAVSYFTGMNPLALIDLMSGIQSTTTEPPI
                     ESVPTGPLKDEIGQFASVVLADTEDTWQQLLPKAGGHYENPRLVLFTGAVHSACGATS
                     SAVGPFYCPQDRKLYLDVSFFKEMSQRLGAPGDFAQAYVIAHEVGHHVQNLLGIAEKV
                     HQLQSRTGTAEGNALSVRLELQADCFAGVWGYHANRERKLIEPGDFEEGLAAAAAIGD
                     DRLQNMSSGYIQPESWTHGSSEQRKTWLRKGLDTGNPEACDTFSSQQL"
BASE COUNT         1655 a         2180 c         2328 g         1544 t
ORIGIN      
        1 cgaagtcaaa ggcgtaaacc gtgtcgtcta cgacattagc tcaaaaccac cgagcaccat
       61 cgaatgggag tgaggtgtgg atgaaagcta tcggtctcca ttgctcgccg aacgcgcaca
      121 ccggatccat cgtattaata gatagccggt gctcgtccaa gcgcgcagtt ggagaccaat
      181 agcttccatc cgggttaagt tatgaaagcg agcttgagaa catctcaata gtttatggaa
      241 gttcgattgc aaaaattgat cgcgggcagc ggcctcgcgt ctcggcgaaa agccgagggc
      301 ttgatcgcgg ctggtcgagt gatggtcaat ggaaagatcg tcacggagct agggaccagg
      361 gtgaatccgg aacgggatca cgtcaaggtc gacggcaagc atctcagcgc agcccagccg
      421 tttgtgtatc tgatgctcaa caagcctcgg aatgtcgtgt cgacgctcga cgatccggga
      481 gggcgcacca ccgtcaagga ttacttgcgt ggcgtgtccg tgcgtgtgtt tccagtcggc
      541 agattggatt tcgacagcga aggactgatg ctgttgacca acaacggaga tcttgcccag
      601 gccctgctcc acccacgtta ccatgtgccc aagacgtatc tgatcaaagt gaagggcgtg
      661 ctgacggatg aagacattgc gcaactggaa cagggtgtgc ggctggagga cgggatgacg
      721 agcccggcgc aggtcaagaa ggtccgaaag gtggaggcga attcctggct ggagatcacc
      781 attcgcgagg gacgtaaaca ccaagtcaag cggatgctcg aatcggtcgg ccatccggtc
      841 atcaaactga tgcggatcag aatggggccg ctctccctcg gccatttgga gccgggcgag
      901 ttccggttct taaccgatcg cgaggcgaat gcgctgcgcc agctcgtaga cgatcggatc
      961 gcactggtag aaggcgaagg ggaaccggtt cgcccatcca ggaggcaacc gaagcgttcc
     1021 gggtgggccc ggtcatccaa gacaaaatcg ccgcgcggtc gcgccgcatg gtcctccatg
     1081 agaaagcgag tctgacgtga aaagcagaac acaccggtgc ggagagttga acagatcgca
     1141 ggtcggccag accgtggtgt tgaacgggtg ggtgcagcgt cgccgcgatc acgggatggt
     1201 gctctttatc gacctgagag accggagcgg tatcacgcag gtcgtcttca atgcagagcg
     1261 caatgccgcc gtgcacaaag cggcgcacac cctgcggagc gagtgcgtcg tgtcggtgac
     1321 cgggacggtg gcggcccgtc cgggtgagtc caggaatccc aatctgtcga ccggtgatat
     1381 tgaagtcttt gcggatgccg tggagatttt gaacgaggcc aaaacgccgc cgttcgtcat
     1441 cgaggacgat gccgaagtga cggaagccat ccgcttgaaa tatcgctatc tcgacttacg
     1501 ccggccgaga atgcagcggt tgttgagctt gcgacacgcg atcatgcagg ggacgcgggg
     1561 cttcttgaat gcggagggct ttctcgaagt tgaaacgccg attctgacga aaagcacgcc
     1621 ggaaggcgcg cgcgactatc tggtgcccag ccgggtcaac cccgggcagt tttttgccct
     1681 gccgcagtca ccacagctgt ttaagcaagt gctcatggtg agcggcgtgg atcgttacta
     1741 ccagattgcc cgttgtttca gggatgaaga tctcagaaac gaccgtcaac ccgagttcac
     1801 acagatcgat ctggagctgt cgtttgttga ccgcggagac gtgatgagtc tcaccgagcg
     1861 aatgattgcc acgatcttcc gcgaggctgg cggcgtacag ctgccgacgc cattcccgcg
     1921 aatgtcgtat gcggaggcga tggggaagta tggaacggac aaaccagacc tccgattcga
     1981 catgccgctg tacgatgtca cggcctttgc cgggacgagc gagttcaagg tgtttcgcga
     2041 agcggccggg aagggaaagc tcgtcaaggc gctcatcgta aaaggtggga gcagcatcgc
     2101 gcgtagccgg atcgatgcgt tgggagaaac cgccaggagc ttcggggcca agggcctggc
     2161 gtggctgaag gtggccagtg atggccaatt ggattcggtc attgctaaat ttcttgatgc
     2221 caaagccttt gctgccgcct tgcctgaagc caagcccggt gacctggtgc tgttcggggc
     2281 ggacaccccc gccatcgtgc atgacgtatt gggccgcatc cggctgctcc tcggagaaga
     2341 gctgggtgtg atcgacaaga cggcatggaa gcccctttgg gtcaccgagt ttcctttgct
     2401 cgattattcg caggaagaga aacgctacgt cttcatgcac aatccctttg ccgctcccct
     2461 cgacgaggac ctggccttgc tggactcgga gccgctcaaa gtgcgcgcca aggcctacga
     2521 catggtgctc aacgggaacg aaatcggcgg aggcagcatc cgcaatcatc gcagtgatgt
     2581 gcaactgcgc atcctggatc tcctgggaat cggcaaggag caggcgcagg ccaagttcgg
     2641 ctttttgctc gatgccttgg attacggtgc gcctccgcat ggcggcatcg ccttcggcct
     2701 cgaccggctg atcatgctgc tcgggggcgc ggagtccatt cgcgacgtca ttgcgtttcc
     2761 caaaacgcaa cgcgcccaat gtcccctcac agacgccccg tcaccggtcg gcgccgatca
     2821 gttgaaggag ttgcgcatca agctcgatct gctggagtga accagagagg atttcccgac
     2881 atggccggca ataccttcgg acacatcttc acggtcacct cttttggcga gagtcacggg
     2941 ccggcgatcg gctgtgtcgt tgatggctgc cctccggggc tcgcgctttc cgctcaggat
     3001 atccaacagg acctggaccg ccgcaagccc ggcacgtcgc gccatgtgac gcagcggcag
     3061 gaagctgata cggtcgaaat tctttccggc gtcttcgaag ggaggacgac gggaacaccg
     3121 gtggccttac tgattcgcaa cgaagacgcg cggagcaaag actacggcag cctcatcgat
     3181 acgtttcgtc ccggtcatgc cgattatacg tattggcaga aatatggaat ccgagatcac
     3241 cgcggcggag gccgtgcgtc tgcgcgcgag actgccgtgc gtgttgccgc ggccgcaatt
     3301 gccaggaagt ggctccggga gacatacggc gtcctcattc acggttacct gagccagctg
     3361 ggcccacacc aggttccgtt taaaacatgg gaggcggtca ccggcaatcc attttttgcc
     3421 ccggatgccg acgtcgttgc gacactcgaa gcgtttatgg atgagctgag gaaatccggc
     3481 gattcggtcg gcgccaggat caccacggtg gccgagcatg ttccggtcgg gtggggcgcc
     3541 ccagtgtatg ccaagctgga tgccgaactg gcggcagcca tgatgagcat caacgcggtg
     3601 aaggcggtgg aaatcggctc agggttcgcc tccgtcatgc aacggggttc agagcatggc
     3661 gacgaactga cgcctgaggg tttcgtcacg aatcatgccg gcggcatcct gggcggtatt
     3721 tcgacgggtc aggatatcgt cgtcacgatc ggcatcaagc caacctcgag catacggatc
     3781 tcccgccgct ccatcgacaa gcagggcaat cccgtgacgg ttgaaacgaa tggacgccat
     3841 gacccctgtg ttggcattcg ggccactccc atcgcggaag ccatgatggc actggtgctc
     3901 atggatcatg cgttgcttca tcgggcacaa aatgcagagg tcaagactgc gactccgaaa
     3961 atcggtggat cggtcgcggc ttcaccctcc ggcaggagag gaaaaccgat tgcgaaggtg
     4021 aatcctgaac cccacgaagc ctgacgtctt gagatgtcga acgcgttgtt ccgcaccatc
     4081 cagcccgcaa ggtttttcca ctgtgtgagc cttctgcggt gtcccctcac ggcatgaccg
     4141 ccccgggttc ttacgacggc cgcatcctcg acatcaaaca cgcgaccatc tatcgcggcg
     4201 atacctgcgt ctttgaagac ctctctttcg tgctggaaga agaccagcat acggcggtgc
     4261 tggggccaaa cggggcaggg aagtctacca tcctgaaact gctggcaggc gaagtgcacg
     4321 cggtgccaaa tgatgtgacg tccatccggc tgtttggcga ggagcagtgg aacgtctgga
     4381 acgtgcggag gcgtctcgga atggtctcgc acgatctcca gcaccagtat atggagcagg
     4441 tgaccggctt gaaagttgtg ctatccggtt attacgcgag catcgggacg tatggacagc
     4501 aggagttctc ctatgcgcac attgccaggg cccattccct gatggaagat ttgggcatcg
     4561 gctttctgac cgaccggcgg tatgccgcca tgtctaccgg cgagcagcgt cgctgtctgc
     4621 tcgccagggc tctcgttcat gggccatcgg tccttgtgct cgatgagccg acgagcggcc
     4681 tcgatttgac ggcaaccttt cactatttgg atcttgtgcg tgggtacatg cggaaaggga
     4741 aaaccattct gctcgtgacg catcacatcc acgaaattcc tccggagatc cgaagagtcg
     4801 tcctgttgaa gaaaggaaag gtcattcagg acggagagaa gaaagcggtg ttgtcggaga
     4861 aacatttgag caacctgttc gattgccgca tcgccttggc ttgtgccaat ggctggtatc
     4921 aggcattacc gggctgacgg actggttccg gcacgccggt gcatgtcctt agatggagac
     4981 ggggactgtc gtcgggaaga gaggggcgac agtccccacg cctagtccgc tagccgccgc
     5041 ccgttagcgt cacggtgaac gtcagatctg cctcggctcg acgcactttc actgcgaccc
     5101 gctggccagg agccgttcgt tcaatcaggt agttcttcaa atgcgcggca tccagaattt
     5161 ccgtgtcctc tatcgccagc aggatgtcat gcttttgaat gcccgcgctt ttcgctggct
     5221 ctgccacatc cttcacggcg atccgccatt tcgtgccgtc cttcactgca gtcatgtgaa
     5281 tgcccagttt tgagaacgcc aagggttttc cttcgatggc ggccgacacg atccgtgcag
     5341 ccaaggtccc gggaatggca aaggccatct gactgcaata tacgtggctt tcatctttgt
     5401 cagtttgaat cacggcatgc aggatgccaa cgacttcgcc ctcaacatta aagagcgcgc
     5461 ctccggaatt gccgctgcat gccgaaagat cggcttggat cagccgcgtc tcaaccgttt
     5521 gaagaaaggt gttggcattg ccgatacggc cgaacgccat tgtcggaccc catcccatcg
     5581 gatacccaac cgtaaagact tcgctgccga ctggcggtgt ttccacggca aatggagcct
     5641 cgacaggcag cacaggacgg tcctgctgat tgagacggta gaccacgacg tccaaatagg
     5701 cgctctctcc aacgatctga gccgcgactt cgtgaagatc agtcgtcaga acatgaatga
     5761 ccttggggac cacctgaccg gacgggctgc ttttctcaac tgcgtgcctg gctgtgacga
     5821 tatagccatc gtgcagatgg aatcccgtgc cgcgaatctt gattttgccc ggcttctctg
     5881 gagcgcgggg atcctgggtg tcttccaata tgccgagcgt cactcgtttg gcacgttcga
     5941 gcgcacccga acgtgtctca tcgccggcgg acgcatggga agccatgagg aggcaggcaa
     6001 ggagggtcag tatggatcgg agaaacgccg gccggttata cgagccacgg cacatgaatc
     6061 atcctttcac tgtatgaggc tggtcttcca cagtataatc agctttcatt ttcaatgaca
     6121 ggggaacgat gcgagcaaca ctcctcacgc tgtgtctggc agtggccttg accccgtctc
     6181 cgctgctctg ggccggggaa gcttccactg gaagcgagac gtcattggcc gacaagatcg
     6241 gtgacaccgc gaaaaagatc ggcaagaaaa tcgaggaagg tgtaacgaag accgccaaaa
     6301 aaattgagga gaagcgcatt cctgaaaagg ttgaacgaaa gctcaagaag gccgtggaca
     6361 aaaccgccga aggcttcggt aaggcggaga agaaactgaa acaaaagctt gccgattaac
     6421 cggcgactcc ggttcaggtc atttcttccg gaagccaaaa aacgatccgg cgcctgctgc
     6481 accggctgat ggaagaaatc ctgccagcag gtttttcaat ccttgggctt cgccatgaag
     6541 ccattggacg gtctgggaaa cctgagcctc aaccgcgttc cagtccacat ccacccagcc
     6601 ggtcgttttc atcgcaccga tgagggcgag accgatgccc accattatcg ctgccattct
     6661 caagaaacgg cgaaaagccc agccgatgag ataaccgcca agatagcttc caccgagttg
     6721 agcgacagtc gaagacgtat agtttccaat ccaagacgcc aacccgccaa tcgtggccac
     6781 tgaaacggcg agaaacgatc ttcccgccca gggaggatca gcgagcattg cctcccagcc
     6841 cgacggtgca ggggaggggg cagcggaaca ggcgtcttct tcgaccatgc gttttttctc
     6901 ctggatctac agctgttgtg acgaaaatgt atcgcaggcc tcgggatttc ctgtgtccag
     6961 ccctttgcgc agccacgtct tccgctgttc agaggagccg tgtgtccagc tctctggctg
     7021 aatatatcca ctcgacatgt tctgcaagcg atcgtctccg atagccgctg ccgcggccag
     7081 tccctcttcg aaatcgcccg gctcgatcaa cttccgttca cggttggcgt ggtagcccca
     7141 gacaccggca aagcaatcgg cttgtaattc caggcgaaca gacagggcgt tcccctcggc
     7201 tgtcccggtc cgactctgaa gttggtggac tttctcggca atcccgagca aattctgaac
     7261 gtggtggccg acctcgtggg cgatcacata cgcctgggca aaatccccag gagcgccaag
     7321 ccgttgtgac atttccttga agaaggagac atcgaggtag agcttccgat cctgcgggca
     7381 ataaaacggc cccactgccg aggatgtcgc gccacaggcg gagtgcaccg cgcctgtgaa
     7441 taacacgagg cgtggatttt cgtagtggcc tcccgctttg ggaagtagct gctgccaggt
     7501 atcttcagtg tcggcgagca ccacagacgc gaattgaccg atctcgtctt tgaggggacc
     7561 cgtcggcacc gactcaatgg gcggctccgt cgtggtgctc tggattccgc tcatgaggtc
     7621 gataagcgcc agcgggttca tgccggtaaa ataactgacg gccagtacca gcacgatgcc
     7681 gccgaggccg agtccgccga ctccgcc
//
LOCUS       DASSCQ010000205        26057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1906217, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000205 DASSCQ010000000
VERSION     DASSCQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 26057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26057
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906217"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /gene="tpiA"
                     /locus_tag="VFH05_13005"
     CDS             complement(<1..727)
                     /gene="tpiA"
                     /locus_tag="VFH05_13005"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET6679188.1"
                     /translation="MAQATVRTTFIVGNWKMNKTASEAAGFVAELSRAIPNNPTVEIV
                     IAPPFTALASVRTALGPASNIALGAQNMHWEQQGAFTGEISGAMLRDLGCTYVLVGHS
                     ERRTLFGEQDGDAHKKVRAALLNGLRPILCVGESLDQRTAGQTESLVTGQLAAALTDL
                     TSNDLERVTIAYEPVWAIGTGHAATTEQAAKVHRTIRAHIAAGWNQATAARMRILYGG
                     SVTPQNAESLLSESEIDGALVGGA"
     gene            complement(730..1923)
                     /locus_tag="VFH05_13010"
     CDS             complement(730..1923)
                     /locus_tag="VFH05_13010"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6679189.1"
                     /translation="MHKQTIDDMSLAGKRVIIRADFNVPLDDSLQITDDTRIRSTLPT
                     INRVVDDGGKAILCSHLGRPKGKFDPKFSLAPIAKRLGRLLGKDVIFAPDCIGPNIEN
                     LVAKMKAGDVMLLENLRFHAGEEQNDEAFSKALASLGDVYINDAFGAAHRAHASTVGI
                     TKYVPIAGAGFLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKRVDKVI
                     IGGGMAFTFLKAKGLEIGNSLVENDMLDFAKGIEEHAFSRGVKFYLPVDCVVAASREP
                     GAETKIVPVQEIPKGWYALDIGPASVKLFTEAVQNAKTILWNGPMGVFEIDAYARGTL
                     SIAHAIADAYALTIVGGGETAMAIHRAGESENISFISTGGGAALELLEGKPLPGLVAL
                     PERKD"
     gene            complement(1986..2999)
                     /gene="gap"
                     /locus_tag="VFH05_13015"
     CDS             complement(1986..2999)
                     /gene="gap"
                     /locus_tag="VFH05_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET6679190.1"
                     /translation="MAIKVGINGFGRIGRNVLRASLGDPDLHFVAINDLTDAKTLAYL
                     LKYDSVHGTLKGSVEAKDDQIIVDGKAIKVLAIKDPKELPWKDLHVEVVIESTGRFTD
                     REGAGKHLSAGAKQVIISAPAKDPDVTIVLGVNEDTFDPKTHHIVSNASCTTNCLAPV
                     AKVLLETFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHL
                     VLPQLKGKMDGLAIRVPTPNVSLVDLTVETEKDCDVAAVNEAFRKASNGALKGILDYS
                     EDPIVSIDQKGDDHSATVDAPLTTVIDKRLVKVIAWYDNEWGYSCRVRDLIKVLAAKS
                     KGR"
     gene            3221..5599
                     /locus_tag="VFH05_13020"
     CDS             3221..5599
                     /locus_tag="VFH05_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET6679191.1"
                     /translation="MTEADQTPQVLEWDKLLELLAAHTRSTRGEACVRSCALAADVED
                     ARRWQQETVEMTMLQDSADPMPTLAFPDVAEALKRAGKGAVLESHELRDQAWVLAVWE
                     EVQRYLGRHAPNAPALGSVAAALLSGPALRKLKHTLDASIDPDGSIRDSATPELRRLT
                     HHANDLKQQIRDQLEHILRSSRYADLLQEQYFAQREGRYVVPIKIDMQRRVAGIVHDV
                     SSSGATVFLEPRELVDLNNAIKVADREVDREVLRILRDLSSRIAEQADVILMGIEALA
                     HLDAIAARAALGRQLRAHPVALNGEGRIRLKQARHPLLVLSKQQVVANDILLDDQVHV
                     LVLSGPNTGGKTVTLKIVGLFALMVRAGMLLPCEPESEMAVFPELFADIGDAQDLARD
                     LSSFSAHMTHMVRLLKGEHGVGRALILLDEPVTSTDPSEGAALAEALLCRFADMGMKV
                     IVTTHYRSLKALAQTTPEFANASVGFDLETLTPTYRLSMGVPGGSSAIDIAGRLGMDS
                     AVLDAARQKLHTDDRRVERLLVDLQAKQRQLTADLAQAVQARTEAEAAAEEAKARLAK
                     LEATEQEQRKGIKKKLQEQFSRARAEVQATLDEVKREQKLVKAKEAKERLVRLEAEAQ
                     ADLGPHVEQIPVEQLESGDQVEIGGLGMTGKLLESPQGKKRVRVKVGEGELLATVANL
                     VGISREREAPQPAAPISRTHRGGERSYHDETPAVVDVRGQAVDEAVDQVLAALDRATL
                     EGAHSLRIIHGHGTGKLKSTLRTYLKSSAYVAHLRAGERHEGGDGVTIVTIR"
     gene            complement(5635..6987)
                     /locus_tag="VFH05_13025"
     CDS             complement(5635..6987)
                     /locus_tag="VFH05_13025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6679192.1"
                     /translation="MHTPIVALVSADPTSRTVLEQSLSDRGGTLLTATTTEAGLRLLA
                     TRQDITLVMCDASSGRISTAAVLAQARQIPGTVPVVIIGRSDTAKAAIEAIREGAFDY
                     LPTPLAADAVNTSIHNAHAIRSSADTPKDLLGFEHIISVSPQMQLVKRLAAEVAATDA
                     TVLITGESGTGKELFARAIHATSLRSKGPFVALNCAGIPEPLLESELFGYQRGAFTDA
                     KQAKPGKFLMATGGTLFLDEIGEMSLSAQAKLLRVLEDHVIDPLGDTSSIKVNIRVIA
                     ATNEDLPAQIKAGRFRLDLFYRLNVYQLRIPPLHERPDDIEPIFSSFLDHARTHHGCR
                     IRAISPEALSLLQRHDWPGNVRELHNIVEGLTISCKSDTIHPEHLPASFRPAKAPSPM
                     VGAEKTSMLAFGLSFHEMEKTILEEALRKAAGNVSQASRLLKITRNTLRYRMAKYHIS
                     "
     gene            complement(7089..8042)
                     /gene="queG"
                     /locus_tag="VFH05_13030"
     CDS             complement(7089..8042)
                     /gene="queG"
                     /locus_tag="VFH05_13030"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET6679193.1"
                     /translation="MSISQAIKREARYLGFDAVGITSVVPEPPTHGLLDRLQEWLRRG
                     YHGTMAWMARDPARRAHPQIVLPGCRSIISLGMNYDSGLRAFEGRGHGRIARYAWGKD
                     YHGVLGDRLALLENKVRDLAPEAITRSYVDTGPVMEKAWAQQAGLGWIGKHSNLVSSD
                     HGSWLVLGEILTTLELPPDVPATDLCGTCSLCIQACPTNAIVEPYVVDATRCISYLTI
                     EFRGSRRDMPVDLTARMGNHIFGCDDCLDVCPFNLRAEPTTEPAFQPDTTTLGPRLDD
                     LAALDEETFAGLFRQSPIRRAKLSGLHRNVAIARQNDERNT"
     gene            8648..9760
                     /locus_tag="VFH05_13035"
     CDS             8648..9760
                     /locus_tag="VFH05_13035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HET6679194.1"
                     /translation="MLATRIRQKEGTFYFIAYNAADLLDKVRFTSRYFFEGEEIAQGK
                     ISEHDDVAQFIAGIERSEKGFQRQLNRQKIKQIVNFYETVVAQPMIPGTVLIFTDETL
                     RFQRAGESESVGHLSEPKGKYLIIDGQHRLAGLHFFRVKHPDQIANVEVPCVLFDGRS
                     GDFATEMFVVINSTHTRINRSHLVDLYEKVSWETPEKKFAAKVVNLLYAEDDSPLQYK
                     INRLGGRSKQEKWILQSEVFNELLKVVVAHKRWMESHLGMKAERCYALVRDYLKGVKE
                     VMEEVWGNNDRYMFTRDVTLKALIRVLDDLIVDRKLINDWDDQRSHKPFAEIVKPWAA
                     LSKEFRAEGFYERFAAKGQLERVRKIHQRLLDAIVG"
     gene            9858..10328
                     /locus_tag="VFH05_13040"
     CDS             9858..10328
                     /locus_tag="VFH05_13040"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6679195.1"
                     /translation="MKTDPAGGVIATARLVFPARPDLIQSLLTDYPHWPDLFEVRMRI
                     VDLQIARGVVTTDLRIDHALLPGERRLVTESRVISGAIYTDLVAGDFKRYHRVWRLAP
                     TNEGRETRAEFELTVAIDSILPDWLVALAMRRELEAHFRIVKEKARALAASSEK"
     gene            complement(10312..11550)
                     /gene="thiI"
                     /locus_tag="VFH05_13045"
     CDS             complement(10312..11550)
                     /gene="thiI"
                     /locus_tag="VFH05_13045"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HET6679196.1"
                     /translation="MQRSFWNSQDTADAKARCRQMRCAIVHYHELALKGRNRDFFEQR
                     LIHNIRSVLKDLGATRIESLRSRIRVVLPEHISDQAAKSRLALIFGIANFALAHSVPL
                     NLLEPDLGELTEGIVHAVKQRTFDTFRVTAKRADKRLPLTSMDVEKAVGQYVCDRTGK
                     KVRLTDPDLIIYVELLAKDAYYYMDKFPGPGGLPVGVSGKVACLVSGGIDSPVAAYRM
                     MKRGCTAVFVHFSGRPLVSRDSEDKVNDLVQTLTTHQSHSKLFIVPFGEIQREIVAQA
                     PAPYRIVLYRRLMVRIASELAQREGCWALVTGDSLGQVASQTPENLSVIEEAAEFPVL
                     RPLIGMDKLEITDEAQRIGTFTTSIEPDQDCCSLFTPSHPSTKTRMDDIRRIERGLDI
                     GSLVKQGVDRVESSEFTFPS"
     gene            complement(11513..12436)
                     /gene="trxB"
                     /locus_tag="VFH05_13050"
     CDS             complement(11513..12436)
                     /gene="trxB"
                     /locus_tag="VFH05_13050"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET6679197.1"
                     /translation="MRNVVIIGSGPAGLTAAIYAARANLAPMLVEGWQSGGQLTTTTE
                     VENFPGFAKGIMGPDLMKEMRAQAERFGTDFLTGDVSSVEFGTRPLKITVDHERTIEA
                     RTVIIATGATAIQIGLKNEARLTGHGVSTCATCDGFFFRGKELVVVGGGDSAMEEANF
                     LTKFATKVYVVHRRDKLRASKIMQDRAFKNDKISFIWNTIVEDILGEDTVSGVRLNNL
                     VMGTTTTLPASGVFVAIGHRPNTALFAGQLNMDAKGYIVTKHGTTTSVPGVFAAGDVQ
                     DSIYRQAITAAGTGCMAAIDAEKFLELTGHR"
     gene            complement(12467..13078)
                     /gene="coaE"
                     /locus_tag="VFH05_13055"
     CDS             complement(12467..13078)
                     /gene="coaE"
                     /locus_tag="VFH05_13055"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET6679198.1"
                     /translation="MILVGLTGGIATGKSTVADLFRRFGAAVIDADQLARDVVQPGKP
                     AWREIVKCFGKRVLLPDRTIDRPALGSIVFRNRRKLRRLERIIHPRVAREQQRRARRI
                     ARRTPNAIIIYEVPLLFEAGVNTRVDKIIVVTADRETQVARLKQRNGLTRRQAFQRIR
                     SQMPLAEKARRADIVLDGTKSKSLLSREIRRLVRDLRARDRPV"
     gene            13177..14187
                     /locus_tag="VFH05_13060"
     CDS             13177..14187
                     /locus_tag="VFH05_13060"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HET6679199.1"
                     /translation="MLMRIIGCVTLWSLVVCAPLHGESGSAQTPRTIVVALDGSGDFR
                     SIQEAVDAAAKGDTVFLKPGRYEQDITIHSKDHVKLVGSGQDKVILVGREDLVGVLHV
                     GKWPYGATNIEISDMTINEHGGHAVGLFNGRGLLLKRLTVNGMLFTQQVEDTRIEDCT
                     IGGSETTGVQFADSQAVLVGNFIHDNDHGVNVAGKSKVRLERNVITRSLFEAVVVNDR
                     AKAVLISNTLVKNGGGAAFLGQSQSDVSGNVVGLNRVGFLVASSSRAATSYNALYNSD
                     GDYLREGNPNQIAPELKAQSDITGDPYFVDPAKGDFRLRADTPLRKIGSFPFLGALPP
                     AE"
     gene            14330..14515
                     /locus_tag="VFH05_13065"
     CDS             14330..14515
                     /locus_tag="VFH05_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679200.1"
                     /translation="MASYKQELEKRGPEGIPSLDDIEPGKICGAVIPMSERAQTQMRD
                     SIEVIDYLLNQERVVWS"
     gene            14622..15170
                     /locus_tag="VFH05_13070"
     CDS             14622..15170
                     /locus_tag="VFH05_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET6679201.1"
                     /translation="MLFLKRLRLSHSYATVRLIATGLVMLFCPAFSFADELPKESVLP
                     LAMASKAAQAAVEACKKDGYRVSASVVDRAGVLRTMGRADGAGSHTVESSRKKAYTAA
                     SLRRATTELAELIGKVPTLQSLREMNDEILILGGGLPIEIGGDVVGGIGVGGAPGAHL
                     DDACAQAGLDAVGAAPKIPTVK"
     gene            15236..15622
                     /locus_tag="VFH05_13075"
     CDS             15236..15622
                     /locus_tag="VFH05_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679202.1"
                     /translation="MPVSAVTQVTPPGVIVTVTRIATHPFLSRFNLALTVSTAGGCVA
                     TSELFPDTGGVSRRNLYVAASDRLYVVGQFDVRRFDLQRCLIELIEFRSVEDGLRFIG
                     SFDIDRSGHWVFHAATTRAERPFEPR"
     gene            complement(16008..17117)
                     /locus_tag="VFH05_13080"
     CDS             complement(16008..17117)
                     /locus_tag="VFH05_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HET6679203.1"
                     /translation="MSPQTRDIVQIDYWPIDIPLTDPFVVATGSRHIAENVCVRMTLS
                     NGTQGYGEAAPFPEVSGETRASCLAAIGLVGRTMLGHRTDQFSNIATRLHEESPAQPA
                     ARCALETAMIDAHCRALGLPLWALWGGADLRERETDITIPIGTLHQTLRLAQAWYAKG
                     FRLFKMKVGADVETDIRRLEAVHRALPEVAFIGDGNQGFSQRECLAFAKGVNRFGGKL
                     VLLEQPVLREDLEALAAIRRETGIPVAADESVRSLVDAKQVVERRAADYINIKTMKTG
                     VIEAHEIALFALASGLKLMIGGMVETRIAMGCSFSLVLGVKGFEVLDLDTPLLLDNDP
                     VIGGYHYRGPCLVPWHRPGLDLRVHSPDAIATLTS"
     gene            17226..20012
                     /locus_tag="VFH05_13085"
     CDS             17226..20012
                     /locus_tag="VFH05_13085"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HET6679204.1"
                     /translation="MKQTFRSMQLMVVLLVVLIGGWLALPSIASTILERWLERQGYED
                     VGVTVGRPGFRSLTVPRMVLTKRLTGEVVTVSLTHAQAEYTLLGLLSGRIDILTLRQL
                     TIEILASPAPDDGQPRPDEGAPDAPDSLLNALTAGDVVQRLPFFPCDEVRLEEIKVFR
                     EQATGPLRTVMMGGTIKHERGALVADMVLQGIDTIPYELRVTGQSAADMSLQLRAAQP
                     EATPFVLWRSQSVPKQAQVHLEGVLEVNVKDLAPFLALALPIGSEWQRAGGNVTIHWT
                     GTAASGVPVSLLWKDPGTEVHATVQTTASLPELKGYGKDLVVKATGTLSGNARLVHWT
                     LAGGSSATAIVGGGAVKGKERLVSLAAYGSQPVRLESAQDTTGELFWGESPPRFTASG
                     PVRIAFGSEKGPIYAECVVSQIYGSGRVIDRAEAKMFMKGALVVAPSERSKNAQLTGE
                     IRGDLAWTGRTFRGTISPSSVASFTDFKQASFHVSGGSVQVEESLPIEVDLSTGRWAS
                     GPGLVAWRSPSIELSGSSLTMQRALTRVDRIEGASMVQRAQMTATLEGLVLAQSSFRS
                     LPVDVTVRVQADPSGVKADIHPKNQHQGIKLTGQLEHQWAEGHGTVHAVVGPLAFNRE
                     TFRLGQLWTPWPYAADVTGGELTGIVDMRWATDSRQRLQIQGGSVDISADRLAGRYRD
                     MLFSGFSTNVKVALEGLQRLSTSRPAEVTVGSLQTGVEVTDLKMTVEGEWDVRERLPL
                     MEIRNIRCEVLGGTVTSQGLRADLAHPPYGLTMLARQLDLQKILTLEQQKGLQGTGVL
                     DGSVPVMVTSRGLTVRDGSFEARPPGGVIRYLASPQATHAVTQANANMEAVLQALNNF
                     QYNVLQVGAEYIEDGTLQLKARLEGRNPDQKKSPPIHFNLTVQENIPALLKSLRLVNG
                     VEDSMRKAFSGSQR"
     gene            20022..20222
                     /locus_tag="VFH05_13090"
     CDS             20022..20222
                     /locus_tag="VFH05_13090"
                     /inference="COORDINATES: protein motif:HMM:NF025003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HET6679205.1"
                     /translation="MVRVVSMVTGGVVGLYLTVGGCTPRVEMATPDKPITINLNVKID
                     HEIRVKVDKELDEVLSQDGGLF"
     gene            20241..20576
                     /locus_tag="VFH05_13095"
     CDS             20241..20576
                     /locus_tag="VFH05_13095"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HET6679206.1"
                     /translation="MRNTHIGRRVAWLVFFLLAVSPAFGISVEEAKTKGLVGERPNGY
                     LGVVNPAAPEAQALANEVNEKRRQAYEDIAKRNRTQLDAVEALAGEKAIQNTKPGYFV
                     EGPGGWTRK"
     gene            complement(20688..21332)
                     /locus_tag="VFH05_13100"
     CDS             complement(20688..21332)
                     /locus_tag="VFH05_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET6679207.1"
                     /translation="MDEPTRLTKTKEQWAKARRGGEEREVFYAGDERLPPGQHVVETF
                     PVLDLGYKPDIPLSTWTITIGGFVASPLTWTWEQFLAQPQFKDVSDFHCVTSWSRYDN
                     QWEGVSFKHILSVVRPLSLAKFVLFKSYDDYTTNLPLEACDDDDVLLTYKWNGKPLAK
                     EHGGPVRMIVPKRYAWKGAKWIKEITFSDKDEKGFWEVRGYSNSALPWDNDRYG"
     gene            21489..21983
                     /locus_tag="VFH05_13105"
     CDS             21489..21983
                     /locus_tag="VFH05_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679208.1"
                     /translation="MKTCLLVLVITGSWWLCMTIVAMEETQAEQRVRKEGSSSGMPAK
                     SLRPVEGRQKQAETKTLSSVKPAAGRKPHRVIKSSKKVPRKILVAQPRTDLIHYGVLE
                     DSQRYDPRPHAHAAGVPHPQTPDLTHDHFQELDRNQDGKIDPVERAFGRIDMDRDLNT
                     RTFR"
     gene            22276..23832
                     /locus_tag="VFH05_13110"
     CDS             22276..23832
                     /locus_tag="VFH05_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HET6679209.1"
                     /translation="MSDMELSGLLPDDQDDRILVENVHPTKWVNPAANGRYNIVVLGA
                     GTAGLITAIVGASLGAKVALVEKHLMGGDCLNVGCVPSKSVIRAARAWADLRNAEQFG
                     LRIPAGVQYDFGAVMARMRKLRARISHNDSAQRYTNLGVDVFIGSGRFASADTIQVEG
                     VAGNRTLRFARAAICTGTRASSPAIPGLKEAGYLTNETVFALTELPQRIGVIGAGPIG
                     CELAQAFARFGSHVYVIEALHGILSNEDRDAAQVVEQQMMKDGVKLLCCGRDLHIVQT
                     ADGKKLSVQSHGQQYDITVDEILVAVGRTPNVEGLGLEEAGVQFDRRGVQVNGRLQTT
                     NPKIFAAGDVCSPYKYTHAADAMAQIVVQNALFPHPFSLGYASIDSLIMPWCTFTDPE
                     IAHVGLYEKDAEAKGIGVETYTFKLDEVDRAILDGQEEGFARVHIRKGSDKILGATIV
                     AAHAGDLINEFSVIMKAGKGARTIAGTIHPYPTQAEVNKKVINLWRKAHFTPATKHKL
                     MRLFAWMRRG"
     gene            23839..24588
                     /locus_tag="VFH05_13115"
     CDS             23839..24588
                     /locus_tag="VFH05_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HET6679210.1"
                     /translation="MRMFHPGTPWLAAVWLLSSSASAATEPPPVLEVGRFASSHAGAP
                     LADGWKPLRFKKVPNHTVYELVEDAGAVVVRATSEASASGLIKEITIDPSAFPVVRWR
                     WKIENLLKHSDVRRKSGDDYPARLYVTFEYDPDKVSFPKKLKYKAGQALFGDIPIAAL
                     NYIWDTKAPIGTVVDNAFTDFAKMIVVESGPAKVGLWVEESRNIYEDYKAAFGEEPPK
                     INGVAIMSDTDNTKERTIAYYGNIQFVPAGQ"
     gene            complement(24688..25083)
                     /locus_tag="VFH05_13120"
     CDS             complement(24688..25083)
                     /locus_tag="VFH05_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679211.1"
                     /translation="MNAKRKTIFPIAIAMVSILAIAVAPALAKDSKKKAPPSHKSAAA
                     AAASMGHVPQVSEAPKRGHHDEKPGPAWKTVGGTVKDIQDNAYTVEDYEGNQVKLYVG
                     QGTKHIKNKKVGDTVRAEITRGGFANSIQ"
     gene            25448..>26057
                     /locus_tag="VFH05_13125"
     CDS             25448..>26057
                     /locus_tag="VFH05_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-A polymerase"
                     /protein_id="HET6679212.1"
                     /translation="MADEREVGLYLVGGVVRDLLLKRSNWDLDLTVEGDGIAFARLVA
                     SRYGAGVALFERFATARLTMSDHTKVDIASTRRESYADPAALPEVRPARLEEDLFRRD
                     FTINAMAIELNDAQWGRLHDPNGGREDLKTKIIRVLHAKSFVDDPTRIFRAIRFAERF
                     GFRLEATTHRLLKQAAETNIVAQLSGPRLANEIFMLMRERHPE"
BASE COUNT         5504 a         7176 c         7836 g         5541 t
ORIGIN      
        1 aagccccgcc gacgagtgcg ccatcgatct ccgactcgct cagcagagat tccgcatttt
       61 gcggcgtcac gcttccacca tacaaaatcc tcatgcgtgc cgctgtcgcc tggttccagc
      121 cggctgcaat gtgcgcgcga atagtccggt ggactttcgc ggcctgctcg gttgtggcgg
      181 catgtcccgt gccgatggcc cacacgggtt cataggcgat ggtgactcgt tcaagatcgt
      241 tggatgtcag atctgtcagt gctgcggcga gctgcccagt cacgagcgat tccgtttggc
      301 cggctgtccg ttggtcgagg gattcaccca cacaaagaat cggacgaagg ccgttgagca
      361 atgccgcgcg aactttcttg tgagcgtccc cgtcctgctc gccgaagagc gttcgtcgct
      421 cggagtgtcc gacgagcacg tatgtgcacc ccaggtcgcg caacatcgct ccggaaattt
      481 cgcctgtaaa ggcgccctgc tgttcccagt gcatgttttg ggcgccaagc gctatgttgg
      541 atgccgggcc cagcgcggtc cgtacggacg ccaatgcggt gaagggcggg gcgatcacga
      601 tttcaaccgt cggattattg ggaatggcac gggacaattc tgcaacgaag ccggcggcct
      661 ccgaggccgt cttgttcatt ttccagttgc cgacgatgaa ggtagtccgc acggtagcct
      721 gggccactgc tagtctttgc gctcaggcag ggccaccaag cccggcaatg gtttgccttc
      781 cagtaattcc agtgccgcgc cgcctcccgt cgagatgaac gagatatttt ccgattcccc
      841 ggcccgatgg atcgccattg cggtttcgcc gccgccgaca atcgtcaagg cgtaggcatc
      901 agcgatggca tgcgcgatgg acaacgtgcc gcgtgcatag gcgtcaattt caaaaacgcc
      961 catagggccg ttccagagaa tggtcttggc gttctgcacc gcctccgtaa agagcttcac
     1021 cgatgcgggc ccgatgtcga gcgcgtacca gcctttcggg atttcctgga cgggaacgat
     1081 cttggtttca gcgccgggct cgcgacttgc ggccaccacg cagtcaacag gcaaatagaa
     1141 tttcacaccg cgcgagaagg catgctcttc aatgcctttg gcaaagtcga gcatgtcatt
     1201 ttccaccagt gagtttccga tctcgagccc ttttgccttt aaaaaggtga atgccatacc
     1261 gccgccgatg atgactttgt ccacccgctt cccgagattc tcaatgacgc cgatcttgcc
     1321 cgaaactttg gctccgccaa gaatggcgac gaacggacgc acagggtttt ccactgcgcc
     1381 ctcgagatat tcgatttcct ttttcaagag gaagccggct ccggctatcg gcacatattt
     1441 cgtgatgccg accgtggagg catgggctcg gtgtgccgcg ccaaacgcat cattgatata
     1501 cacgtcgcca agcgaggcga gagccttgga aaaggcttcg tcgttctgct cttctccggc
     1561 atggaaacga agattctcga gcagcatgac gtcgcccgct ttcatcttcg cgacgaggtt
     1621 ttcgatgttc gggccgatgc agtccggcgc aaagatcacg tcctttccca acagtcttcc
     1681 cagccgtttg gcaataggcg cgaggctgaa ctttggatcg aacttgcctt tcggccggcc
     1741 gaggtgcgaa cacagaatgg ctttgccgcc gtcgtcaacc acccggttga tggtcggaag
     1801 ggtggaacgg atacgggtat cgtcggtaat ctggagggaa tcgtccaagg ggacattaaa
     1861 gtctgcgcgg atgatgaccc gcttgccggc cagggacatg tcgtcgatcg tttgcttatg
     1921 cagattcatg gcgatcccta ttggtcagcg tgaagcaggc cgacgtgcag tgggggagtt
     1981 gcggtttagc ggcccttgct cttggccgcg agtactttaa tgaggtcgcg aacgcggcac
     2041 gagtaacccc attcgttgtc gtaccaggcg atgaccttga cgagtcgttt atcaatgacc
     2101 gtggtcaacg gcgcatccac cgtggccgag tggtcatcgc ctttttgatc gatcgagaca
     2161 atcggatctt cggagtagtc caggatgcct ttcaaggcgc cgttcgaggc cttgcgaaac
     2221 gcctcattga ccgctgccac atcgcagtcc ttttcggttt cgacggtgag gtccaccaat
     2281 gagacgttcg gggtgggaac gcgaatcgcg agaccgtcca tcttgccttt caattgcggc
     2341 aacacgagat ggagcgcctt tgcggcgccg gtactcgtgg gaatcatgga catgccggct
     2401 gcacgcgctc gccgtagatc cttgtgagga agatcgagca attgttggtc attggtatag
     2461 gaatggattg tggtcatgac tccatgcttg atgccgaagg tctcgagcag gactttggcc
     2521 acgggagcga ggcagttggt cgtgcaggat gcattgctca cgatatggtg tgtcttggga
     2581 tcgaacgtgt cttcattgac ccccaagacg atcgtcacat ccggatcctt cgcgggggcg
     2641 gagatgatga cttgtttggc gccggccgac aggtgcttgc ccgctccttc gcgatcggta
     2701 aagcggccgg tcgattcgat gaccacttcg acatgcaaat ccttccaggg cagttctttc
     2761 gggtccttga tggccagcac cttgatcgct ttgccgtcaa cgatgatttg gtcatccttg
     2821 gcctcgacgc ttccctttag ggtgccgtgc acggagtcgt acttcagcaa ataggcgagc
     2881 gtcttggcat cggtcagatc attgatggcg acaaagtgca aatcgggatc gccgagtgag
     2941 gcacgcagca cattacgccc gattcgtcca aacccattga taccgacttt gatagccatg
     3001 ataaagcagt ccctcctaaa ctcggccgcg tgtgtcccgt tgaaacgacg gccgatagta
     3061 tcggcggttc accctctgag tcaagcgata caaaagtggt aaccccgcgt ccgtagtgag
     3121 gttttgactg acttctggct tgtcgcacct gccggggcgg gccgccttgc gactcgctcc
     3181 cgggtccgtt acagtttttg actgattcac ttgagtggcc atgacggaag ccgatcaaac
     3241 accgcaggta ttggaatggg acaagctcct cgaattgctt gcggcccata cgcgctccac
     3301 tcgcggcgag gcatgcgtga ggtcctgcgc tttggccgca gacgttgaag acgccaggcg
     3361 ctggcagcag gaaaccgtgg agatgaccat gctccaggac agtgccgatc cgatgccgac
     3421 attggcattt cccgatgtcg cagaggcgct gaagagggcc ggcaaaggtg cagtactaga
     3481 gagccacgaa ctgcgcgatc aggcctgggt cctagccgtg tgggaagagg tccaacgcta
     3541 cctcggacgg catgcgccga atgcgccggc cctgggcagc gtcgctgctg cgctcctgtc
     3601 cggtcctgct ttgcggaagc tcaaacacac actggacgcc tcgatcgacc cggatggttc
     3661 catccgagat tcggcaacgc cggagctgcg ccggctcact catcacgcga acgatctcaa
     3721 acagcagatt cgcgatcaac ttgagcacat tctacgttcg agtcgatatg cggatcttct
     3781 tcaggagcag tatttcgcac agcgggaagg ccgctacgtg gtgccgatca agatcgacat
     3841 gcagcgccgt gttgcaggca tcgttcacga cgtctcgtcc agcggcgcca cagtgttttt
     3901 ggaacctcgc gaattggtcg acctgaacaa cgcgatcaag gtggccgacc gtgaggtcga
     3961 ccgggaagta ctgcgaatcc ttcgcgactt gtccagccgc attgccgaac aagccgacgt
     4021 catcctgatg ggcatagagg ccctcgctca cctggatgcc atcgcggctc gggcggcatt
     4081 gggacgacag ttgcgggctc atcctgttgc gctcaacggg gagggccgca tacgcctgaa
     4141 gcaggcgagg catccgttgc tcgtgctctc caagcaacaa gtcgtggcca atgacatcct
     4201 tctggatgac caggtccacg tcctcgtgct atccggacct aatacgggcg ggaaaaccgt
     4261 cacgctgaaa atcgtgggcc tctttgctct catggtcaga gccggcatgc tgctgccgtg
     4321 cgagcccgaa tccgaaatgg cggtgttccc ggagctcttt gcggacatcg gagatgcgca
     4381 ggatctcgcc cgcgacttgt cgagcttctc cgcacacatg acgcacatgg tccggctgct
     4441 caaaggcgag catggcgttg gtcgtgcctt gatcctgctc gacgagccgg tcacgtcgac
     4501 agatccgagc gaaggggccg cgctggcgga ggccttgctg tgccgttttg ccgacatggg
     4561 gatgaaggtc atcgtgacga cgcactaccg ttcgctcaag gcgttggcgc aaacgacgcc
     4621 ggaatttgcc aatgcgagcg tcggattcga tctggagacg ttaacaccta cctatcggct
     4681 gtccatggga gttcccggcg gctcgtcggc aattgacatt gccggacgtc ttgggatgga
     4741 ctccgctgtc ctggacgcgg cgagacaaaa attacacacc gatgatcggc gggtggaacg
     4801 gctgctcgtg gacttacagg caaaacagcg gcaattgaca gcagatcttg cccaggcggt
     4861 tcaagcgagg accgaagctg aggctgcggc ggaagaggct aaagcgcgac tggcgaagtt
     4921 agaggcgact gagcaggaac agcgcaaagg cattaaaaag aaattgcagg agcagttcag
     4981 ccgcgcacga gcggaggtgc aagcgacgct ggacgaagtc aaacgtgagc agaaattggt
     5041 caaagcgaaa gaagccaagg agcggctcgt tcggcttgaa gcggaagcgc aagccgattt
     5101 gggccctcac gtcgaacaga ttccagtcga gcagctggag tctggggatc aggtggaaat
     5161 cggcggcctg gggatgactg gcaagttgct cgagtctccg cagggaaaaa agcgcgtccg
     5221 cgtcaaagtc ggtgaggggg agcttttggc aaccgtggcc aatctggttg gcatcagtcg
     5281 agaaagggaa gcgccgcagc ctgccgctcc gatttcgcgc actcaccggg gaggagaacg
     5341 ctcatatcat gacgaaactc ctgcggtggt cgatgtccgc gggcaggcgg ttgatgaagc
     5401 tgtggaccag gtgttggccg ccctggaccg cgccactctg gaaggagcgc actcgctgcg
     5461 gattatccat ggccacggca ctggtaagct caaatcaacc cttcgaacgt atctgaaaag
     5521 ctcagcatac gtggcacacc tccgtgcggg cgagcgtcat gagggcggtg acggagtcac
     5581 aatcgtcacc atacgctagg ctcaggtagg gctcaaaacc gacgtatcac gtggctaaga
     5641 gatatggtac ttggccattc ggtagcgaag ggtattgcga gtgattttga gtaaccggct
     5701 ggcttgggaa acgttccctg cggccttacg gagcgcttcc tccaaaatgg tcttctccat
     5761 ttcatgaaac gagagtccga acgctaacat cgacgttttt tccgccccca ccatcggtga
     5821 aggcgctttt gccgggcgga acgaagccgg taaatgctca gggtgaatgg tgtcgctctt
     5881 gcagctgatc gtgagtcctt ccactatgtt gtgcagttcg cgcacgttgc cgggccagtc
     5941 atgccgctgt aacagcgaga gtgcttcagg agagatggcc cgaattcggc agccatgatg
     6001 cgttcgtgca tgatcgagaa aagatgagaa gatcggctca atgtcgtcgg gccgttcatg
     6061 cagcggaggg atccgcagtt gatacacatt taagcgataa aacaggtcaa gtcgaaagcg
     6121 tcccgcctta atttgagcag ggaggtcttc attggtggcc gcgatgactc gaatgttgac
     6181 cttgatgctg ctggtatcgc cgagcggatc gatgacatga tcttccaaga cacgcagaag
     6241 cttggcttgc gctgagaggc tcatctcacc gatttcgtcc agaaagagcg ttccgccggt
     6301 cgccatcaga aacttgccgg gcttggcctg cttggcatcg gtaaatgcgc cgcgctggta
     6361 gccgaataac tcggattcga gcaacggctc gggaataccg gcacagttga gggccacaaa
     6421 gggacctttc gatcgcaggc tggtcgcatg gatcgcacgc gcgaatagtt ccttgcccgt
     6481 tccgctctct ccggtgatca ggaccgtggc atcggtggca gcgacttcgg cagccaatcg
     6541 tttgacgagt tgcatctgcg gagacacgct gatgatatgc tcaaatccca gcaggtcctt
     6601 tggagtgtct gctgaggaac ggatggcatg ggcattatga atggacgtat tgacggcgtc
     6661 cgcagcgagt ggggtgggaa ggtagtcaaa ggctccttcg cggatcgcct cgatggcggc
     6721 tttggcggta tccgaacggc caatgatgac gacgggaacc gtaccgggga tctgtcgggc
     6781 ttgtgccagc acggcagcag tggaaatacg gcccgaactg gcgtcgcaca tgaccagcgt
     6841 gatgtcctgt cgtgtggcga gaaggcggag tccggcttcg gtggtcgtcg ccgtcagaag
     6901 cgtgccgccg cgatccgata gcgactgttc gagaaccgtt ctcgatgtgg gatctgcaga
     6961 aaccagtgca acgatcgggg tgtgcataac aacgctccac cgcgagctgc cgaacaacga
     7021 ggttagccta ctcctatttc caggctgatg gaagaggcaa ggctttgctg cagactaaga
     7081 gttagcatct aagtattcct ctcatcgttc tgccgcgcaa ttgcgacgtt acggtgcaag
     7141 ccggacagct tggcccttcg aatcggactc tggcggaaga gtccagcgaa agtctcttcg
     7201 tcgagggcgg ccaagtcgtc caagcgggga cccaacgtcg tggtgtcggg ctgaaacgcc
     7261 ggttccgtcg ttggttccgc cctgagattg aacggacaga cgtccagaca gtcgtcgcat
     7321 ccgaaaatgt ggtttcccat acgggccgtc agatccaccg gcatatcgcg gcgagagcct
     7381 cggaactcta tcgtcaaata cgaaatgcag cgtgttgcat cgacgacata cggttccaca
     7441 atggcattgg tcgggcacgc ctggatgcac aacgagcacg tcccgcacag atccgtcgcg
     7501 ggaacatccg gcggcaattc cagggtggtc aggatctccc cgagcaccag ccacgagccg
     7561 tggtctgagg aaacgaggtt ggagtgtttg ccgatccacc caagaccggc ctgttgcgcc
     7621 caggccttct ccatcaccgg ccccgtatcc acgtaagacc gtgtgatggc ttcgggcgcc
     7681 aaatcgcgca ccttgttttc aaggagcgcc agacgatcgc ccaatacgcc atggtagtcc
     7741 tttccccatg catagcgggc gatacggcca tgtcctcgtc cctcaaaggc acgaagtcca
     7801 ctgtcgtagt tcattcctaa tgagatgatt gagcggcatc caggcaggac gatctgagga
     7861 tgtgcgcgtc gggcaggatc gcgcgccatc cacgccattg tcccgtgata gcctcgccgc
     7921 agccactctt gaaggcgatc gagtaacccg tgcgtcggtg gttccgggac cacggaggta
     7981 atgccgacgg cgtcaaaccc cagatatcga gcttcgcgct ttatggcttg gcttatcgac
     8041 atggtgagtc agacgactcc tggacatcgc caccacgtgc tagaaagtgg tagtccagca
     8101 atgtgcccgc catcgtcttg cgggcggagc gcaggaagcg tttatgatcc ttgctagtgg
     8161 gggttgcagt cgaatcggac gatgaactgg acggaacagt gaccggtgct gcaacgaccg
     8221 caagtacctg tgattttggt cttccaatcc gtctgttttt cgtcgaagcg gcagaaggtc
     8281 tttccccctt tggaaatcgt cgtccaaggt ttgtcagtgc cgggtgcgtt cgccaccaga
     8341 cagccgtgag gaattgggac ctggcaatgg tgggagggtt cgcctttggc catcccaaaa
     8401 ctacacgcct gttcggtagt ggcgggaaac tccgaaggca cctggctttg agcacccgcc
     8461 ggccgtaaca ggaagaaggc aagaagcatg agcccgccga gctttaggtg gaatggcatc
     8521 acgcgcatgc acggatcgta tcacagtctc tttgcttgcg gccaccgact cgactgcgta
     8581 gaatctgtca gggtaacggc ggtgttcggc tatcgggacc tcgggccagt cattagggga
     8641 gggtaccatg cttgcgacgc ggatccgtca aaaggaaggc accttttact tcattgctta
     8701 caatgcggcc gatctcctgg acaaggtccg cttcaccagt cggtactttt tcgagggcga
     8761 ggaaatcgcg caagggaaaa tctctgagca tgatgatgtg gcgcagttca ttgctggtat
     8821 tgagcggagt gaaaagggct ttcaacgtca actgaaccgg caaaagatca agcagatcgt
     8881 caatttctac gagactgtcg tcgctcaacc tatgattccc gggacggttt tgatcttcac
     8941 ggacgagacg ctgaggttcc aacgcgcagg ggagtcggag tccgtcgggc atttgagcga
     9001 gccgaaaggc aagtacctga tcatcgacgg ccaacatcgg ttggcaggac ttcatttttt
     9061 tcgggtgaag catcccgatc aaattgccaa tgtcgaggtg ccgtgcgtct tgtttgacgg
     9121 ccgtagcgga gactttgcga ccgaaatgtt cgtcgtcatt aacagtactc acacccgcat
     9181 caatcgttct cacttagtcg acctgtatga aaaggtctcc tgggaaacgc ctgagaagaa
     9241 gtttgctgcc aaagtcgtca acctgctcta cgcggaggat gacagtcctc ttcagtataa
     9301 gatcaaccgg ttgggcggca ggagtaagca ggaaaaatgg atcctgcagt cagaagtctt
     9361 caatgaactg ctcaaggtag tggtcgctca caaacgttgg atggaatcgc atcttggtat
     9421 gaaggcggag cggtgttatg cgctggtccg cgactatttg aaaggcgtga aagaggtgat
     9481 ggaggaagtg tggggtaaca atgatcgata catgtttacc cgcgacgtca ccctcaaagc
     9541 gttgattcgt gttcttgacg acctgatcgt ggatcgcaag ctgatcaatg attgggatga
     9601 ccagcggtca cataagccgt ttgcggagat tgtcaaaccg tgggccgcct tgtccaaaga
     9661 atttcgcgcc gaaggatttt acgagcgatt tgccgccaaa ggacaactcg agcgggtccg
     9721 gaaaattcat cagcggctgc tggatgcgat cgtcgggtag cgtggttccc tctgtatgtt
     9781 ggttcctcat cctctgcatc tgctatctgt ggccaagcct tccggcctcg gctgaggctc
     9841 tgcaggagca tctcgaggtg aagacggatc ctgccggcgg agtgatcgcc acggcccggc
     9901 ttgtgtttcc ggccaggcct gacctcattc aatcgctgct gaccgactat ccccattggc
     9961 ccgatctctt tgaagtgcgg atgcggatcg tcgatttgca gatcgcccgc ggcgtcgtga
    10021 ccaccgatct gcgcattgac cacgctttgt tgcccggaga gcgccggctg gtcacggaat
    10081 cgcgcgtgat atcgggagcg atctatacgg atcttgtcgc gggagacttc aagcgctatc
    10141 accgcgtatg gagattagcg cccacgaacg aaggacggga aacgcgcgcc gaattcgaac
    10201 tgacagtcgc catcgattcc atcctccccg actggcttgt ggccttggca atgaggcgcg
    10261 agcttgaagc gcatttccgg atcgtgaagg aaaaggcgcg ggcactggcc gctagctcgg
    10321 aaaagtgaac tccgaggatt cgacccgatc cacgccctgt ttgacgagcg agccgatatc
    10381 gagaccgcgt tcaattcggc gaatgtcatc catccgggtc ttggtactgg ggtgcgaagg
    10441 cgtaaagagg gagcaacagt cctgatccgg ctcaatcgaa gtcgtgaacg tgccgatccg
    10501 ctgcgcctcg tcagtgattt cgagcttgtc catcccgatc agcgggcgaa ggacaggaaa
    10561 ttccgccgcc tcttcaatga ctgacaaatt ttccggtgtt tgtgaggcca cttggccgag
    10621 actatcgccg gtcaccagag cccagcaccc ttcccgttgc gccaactccg aggcaatgcg
    10681 gaccatcaga cgacgataga gcacaatacg gtacggtgcg ggagcctggg ctacgatttc
    10741 ccgttgaatt tcgccaaagg gcacgatgaa gagcttggaa tgagactgat gggtagtcaa
    10801 ggtttgaacg agatcgttga ccttgtcctc cgaatcccgg ctcaccagcg gacgtcccga
    10861 aaaatgcaca aagactgcgg tgcaaccccg tttcatcatg cgataggccg ccaccggtga
    10921 atcgatcccc ccggagacca gacaggcgac cttcccactc acgccgacag gcaggccgcc
    10981 cgggccgggg aacttgtcca tataatagta ggcgtctttc gccagcaact cgacatagat
    11041 gatcaggtcc gggtcggtga gcctgacttt cttgcctgtg cggtcgcaga catattggcc
    11101 caccgctttt tcgacgtcca ttgacgtcag cgggagacgt ttatccgctc gcttggcggt
    11161 cacacgaaag gtgtcgaagg tccgctgctt gacggcgtgt acgattcctt cggtcagctc
    11221 gccgagatca ggctcgagca ggttgagtgg cacggaatgc gcgagggcaa agttcgcgat
    11281 gccaaagatg agcgccaagc ggcttttcgc ggcttgatcg ctgatatgct cagggaggac
    11341 gacgcggatc cggctgcgta gactctcgat gcgagttgca cccagatctt tcaaaacgct
    11401 gcggatgtta tggatgaggc gctgctcgaa aaaatctcgg tttcggcctt tgagggcgag
    11461 ttcgtgataa tgaacgatcg cgcagcgcat ctgtcggcat cgggccttcg cgtcagcggt
    11521 gtcctgtgag ttccaaaaac ttctctgcat cgatcgccgc catgcagcct gttccggccg
    11581 cggtgatcgc ttgccggtat atcgaatcct gcacatctcc tgcggcaaac acgcccggca
    11641 cactggttgt cgtgccgtgc ttcgtcacga tgtacccctt ggcgtccatg tttaattgtc
    11701 cggcaaacaa ggccgtgttc gggcgatgcc cgatggccac gaacacccca gaggctggca
    11761 gcgtcgtcgt cgttcccatc accaagttgt tgagccgcac gccgctgacc gtgtcctcac
    11821 cgaggatatc ttcgacgatc gtattccaaa tgaaagagat cttgtcgttt ttaaaggcgc
    11881 gatcctgcat aattttggaa gcccggagtt tatcccgccg atgcacgacg taaactttag
    11941 tggcaaactt tgtcaaaaaa ttcgcctctt ccatcgcact gtctccccct ccgacaacca
    12001 ccaactcctt cccccgaaag aaaaatccgt cacatgtggc gcaggtggag acgccgtgcc
    12061 cggtgagcct ggcttcattc ttcagtccga tttggatggc ggtggcgcct gttgcgatga
    12121 tcacggttcg tgcttcgatt gttcgttcat ggtccaccgt gattttcaaa ggccgtgtgc
    12181 caaactccac ggagctgacg tcaccggtca gaaaatccgt cccgaaccgc tccgcttgtg
    12241 cgcgcatttc tttcatcagg tccggcccca tgatgccttt cgcgaagccg ggaaaattct
    12301 ctacttcggt cgtggtggtc aactgcccgc cggattgcca gccttcaacg agcatcggag
    12361 cgagattggc tcgagccgcg tagatcgctg cagtgaggcc agccggacca gagccgataa
    12421 tgacgacgtt gcgcatacag tcaatctagc acagcaaaca acgggattac acagggcggt
    12481 ctcgtgcacg caaatcgcga acgaggcggc ggatctctct gctcaggagg gatttgcttt
    12541 tggtaccgtc caacacgatg tcggcgcgac gcgccttctc cgcaagcggc atctggcttc
    12601 tgatacgctg gaatgcctgt cgcctcgtga ggccgttgcg ctgcttcaac cgggcgactt
    12661 gggtttcacg gtctgctgtg accacgataa ttttatccac gcgcgtgttg acgccggctt
    12721 cgaacaagag agggacttcg taaataatga tggcattggg agtgcgtctg gcaatgcgac
    12781 gcgcacggcg ttgctgttcg cgtgcgaccc gtggatgaat gattcgctca aggcgccgga
    12841 gctttcgccg gttgcgaaag acaatggagc cgagagcagg acgatcaatc gtccgatcgg
    12901 gcagcaggac tcgtttgcca aagcatttca cgatttcgcg ccaggccggt ttgccgggct
    12961 ggaccacgtc acgggccaac tgatcggcat cgataacggc ggcaccaaag cgtctgaaga
    13021 ggtcggcgac cgtgcttttt cccgttgcga ttcctcccgt caatcccacg aggatcatgc
    13081 ggcgaagaat accatgtagg tgttaagaaa gtcgtcgagc cgcttcgaac tcacgattga
    13141 cttcggacca gagccgcctg tatgaacgtg agaattatgc tgatgcggat aatcggctgc
    13201 gtgaccttgt ggtcacttgt tgtgtgcgct ccccttcacg gggagagcgg atctgcgcag
    13261 actccgcgta ccattgtggt cgctttggac gggagcggtg acttccggtc gatacaggag
    13321 gccgtcgatg cggcggcaaa gggcgacacg gtcttcctca aaccgggacg ctatgaacaa
    13381 gacatcacga ttcacagcaa ggatcacgtc aaactcgttg gatccgggca agacaaagtc
    13441 atcttggtcg gccgtgaaga tttagtggga gtccttcatg taggcaaatg gccgtacggc
    13501 gcgacaaaca ttgaaatcag cgacatgacg atcaacgagc atggcggtca tgcggtgggg
    13561 ctgttcaacg gccggggcct attgctcaag cgtctcacag tgaacggcat gctgttcact
    13621 cagcaggtcg aagacacgcg cattgaagac tgcaccattg gggggagtga gacgacgggt
    13681 gtgcagtttg cggactctca agcagtcttg gtcggcaatt tcatacatga caatgaccac
    13741 ggggtgaacg tcgcgggcaa atcgaaggtt cgtctggaac gaaacgtcat caccagaagt
    13801 ctgtttgagg ccgtggtggt caatgatcgc gccaaagccg tactgatcag caatacgctg
    13861 gtcaaaaacg gcggcggggc ggcctttctt ggccagtccc agagcgatgt cagtggcaat
    13921 gtggtcggcc tcaatcgagt ggggttcctc gttgcatcgt ccagtcgagc ggctacgtcc
    13981 tataacgccc tatacaatag cgacggtgat tatctccgag agggaaatcc caatcaaatt
    14041 gcccccgagc tcaaggcgca atcggatatc actggtgacc cgtattttgt cgatcccgca
    14101 aagggtgatt tccgactccg tgcagacaca ccgttgcgaa aaattggctc tttccccttc
    14161 cttggcgccc ttcctcctgc tgagtaaact acttagtatt caacggctta tgcggctagc
    14221 ctggttgctg ccaattcgtg tgctaagcta tcagtacgcc aggtgtcgaa ggactggcgt
    14281 gacaattgcg aaacgggcca ttggacgggc cataggtagg aggcacagcg tggctagcta
    14341 caaacaagag cttgagaagc gcgggccgga aggtatccca tctctcgatg acattgaacc
    14401 gggtaaaatc tgcggggccg tcattcccat gtccgaacgg gcacagactc agatgcggga
    14461 tagcatcgaa gtcatcgatt atctcttgaa ccaggaacgt gttgtctgga gttaaacacc
    14521 gcatctttcc tcttatcacc gtagactccc cttgacacct cttccctctt cgtagtctct
    14581 tcttggcatg tccgtccacc tgctcaagag gagatgcctg catgttattt ctgaaacgtc
    14641 ttcgtctatc ccactcgtac gcgactgtgc gcttgattgc gaccggtcta gtcatgcttt
    14701 tctgtcccgc attttcattt gcagatgaac tgccgaaaga atccgtcttg ccgttggcaa
    14761 tggcgagcaa ggcggctcaa gctgccgtgg aagcctgcaa aaaagacggg tatcgcgtca
    14821 gtgcttctgt ggtggaccga gccggtgtgt tacggactat gggacgggcc gatggagccg
    14881 ggtcccatac ggtcgagagc agcaggaaaa aggcctatac agcggcaagt cttcgccggg
    14941 cgaccacgga actggcagaa ctgattggga aagttccgac gcttcagtcg ctgagggaga
    15001 tgaatgacga aatcctcata cttggcggcg gtctgccgat tgaaatcggt ggcgacgtcg
    15061 ttggcggcat cggggtgggc ggtgcaccgg gggcacatct ggacgatgct tgcgctcaag
    15121 ccggacttga tgctgtgggg gcggctccca aaattccaac ggtcaagtga tcggcagctg
    15181 gggaaggggc agcctgcttg gttgtatcgg gctcctcaat ggatgtaaca gcgacgtgcc
    15241 tgtgtctgcg gtcactcagg tgacgccgcc cggagtcatc gtcaccgtca cgcgcatcgc
    15301 cacgcatcca ttcttgtctc gcttcaatct cgcactcacg gtgagcacag ctggtggctg
    15361 tgtcgcaacc agcgagctgt tccccgatac cgggggtgta agtcggcgca atctctatgt
    15421 cgccgcatcg gatcgactgt atgtggttgg gcagtttgat gtgcgccggt tcgacctgca
    15481 gagatgtctc attgaactga tcgaatttcg atcggtggag gatggcctga ggttcatcgg
    15541 ctcgtttgac atcgatcgtt caggtcactg ggtgtttcac gcagccacca ccagggcaga
    15601 acgtccgttt gagccgcggt gacactaccc cacaggcgat gcactgtacc accaaggtcg
    15661 tgatggacca acagatcgtc ggataccacc aagatgaagt cggggattgg gttgcggacc
    15721 tcgcctgcgg ccacggacag cacgtgcgtc atcggccacc gatgaccaac cggccttggg
    15781 tgttgactca atccggtcga gaccagcatc tgggcagcat actgaactgt ctgaagtgtg
    15841 aagagccgga gacctgaatc agatcgaatc ccccctggag ctgcacaccg ttttttacgt
    15901 gcaagctcac caaggcgtgc agggatggct tcccgcctcg cggtggccga agggcaacgg
    15961 tgtaatacgg aacagtcagt tccgtgtgat cgccgggact ggcatcgtta gctggtgagg
    16021 gtggctattg cgtcaggaga atgaacacgc aggtctagcc cgggtcgatg ccagggcacg
    16081 aggcaaggcc cacggtagtg atacccgcct ataactggat cgttgtccaa aagcagcggg
    16141 gtatccagat cgaggacctc gaaccctttt acaccgagca ccaggctgaa tgagcagccc
    16201 atcgcaattc tcgtttcgac catgcccccg atcatgagct tgaggccgga ggcgagggcg
    16261 aacagggcga tttcatgagc ctctatgaca ccagttttca tggtcttgat attgatgtaa
    16321 tccgccgcgc gccgttcaac tacctgcttc gcgtccacga gagatcggac ggattcgtcg
    16381 gccgcaacgg gaatacccgt ctctcgccgg atggcagcaa gcgcctctaa gtcctctcgg
    16441 aggaccggct gttcgagtaa gaccagcttg cccccgaacc ggttcactcc ttttgcgaac
    16501 gcgagacatt cccgctgaga aaatccctga ttcccatccc cgatgaaggc gacttccggc
    16561 aaggcacggt ggacggcttc caatctccgg atgtcagtct cgacatcagc acccactttc
    16621 atcttgaaga gccggaaccc ttttgcgtac caggcttgcg ccagcctcaa cgtctggtgc
    16681 agggtgccaa tagggatggt aatgtccgtc tcgcgttccc gtaggtcggc gccaccccac
    16741 aaggcccaca atggcagccc cagcgcacgg caatgcgcat caatcatggc cgtttccagt
    16801 gcgcaacgtg cggccggttg ggccggtgac tcttcatgca accgcgtcgc aatgttgctg
    16861 aattgatccg tccgatggcc cagcatggtg cgcccaacca ggccaatggc agcgaggcag
    16921 gaagcacggg tctcgccgct aacttcggga aagggtgcgg cttctccgta tccttgtgta
    16981 ccattggata gggtcatgcg tacacagacg ttctcggcga tgtggcgcga cccagtggcc
    17041 acgacaaagg ggtccgtcag aggaatgtcg atgggccagt agtcaatttg tacgatgtct
    17101 cgagtctgtg gtgacatggc cgcggcatct taacgagaaa cgagtatgat gccaatgaga
    17161 aggatctggg ctgtgaatca ccgccggtat ggtgggtcga tgcagacggc ctgtgatgat
    17221 ccatcatgaa acaaacgttc cgttccatgc agctcatggt cgtgttgctg gtggtcttga
    17281 ttggggggtg gctcgcttta ccttcaattg cctcaaccat ccttgaacgg tggttggaac
    17341 ggcagggcta cgaggatgtg ggcgtcacgg tcgggcgtcc cggctttcgg tctctgactg
    17401 ttccgcgcat ggtcctgacg aagcgactga cgggcgaagt tgttacggtc tctctgaccc
    17461 atgcccaagc cgagtatacc ttgctgggct tactgtccgg tcgcatcgat attctcaccc
    17521 tacggcagct cacgatcgaa atcttggcgt caccggctcc agacgacgga cagccgagac
    17581 cggacgaagg tgctccggat gcgccggatt ccttgctcaa tgcgctcacg gccggtgatg
    17641 tcgtccaacg gttgcctttt tttccgtgtg acgaagtcag gttggaggaa atcaaggtgt
    17701 tccgtgaaca ggctacaggt cctctgcgca cggtcatgat gggaggtacc atcaaacacg
    17761 agcgcggggc gttggtcgct gacatggttc tgcaaggcat tgatacgatt ccctatgagc
    17821 tacgcgtgac cggccagtcc gcggccgata tgtccctgca actccgagct gcccaaccgg
    17881 aggccacccc cttcgtgttg tggcgatcgc aatcggtccc caagcaagca caagttcatc
    17941 tcgaaggagt gttggaggtg aatgtcaagg accttgcgcc gtttttggct cttgcgctgc
    18001 cgattggcag cgagtggcaa cgggcgggtg gcaatgtgac gattcattgg acaggcacag
    18061 cggcctcggg ggtgccggtc tccctgttgt ggaaggatcc cgggaccgaa gtccatgcga
    18121 ccgttcaaac tacagcctca cttcctgagc tcaaagggta tggaaaagat ctcgtggtca
    18181 aggcgacggg cacgctctcc ggcaacgccc gactggttca ttggacgctc gcgggcggat
    18241 cgtcggcaac ggctatcgtg ggtggggggg cagtcaaagg aaaggaacga cttgtaagcc
    18301 ttgccgcata tggatcgcaa ccggttcgtc tcgagagcgc ccaagacacg acgggcgagc
    18361 tgttttgggg cgagtcgcct cctcgattta cggcctcggg gccagtgcgc attgcgtttg
    18421 gttccgaaaa aggtccgatc tatgccgaat gtgtggtgtc gcagatttat ggtagcggac
    18481 gcgtgattga tcgtgctgaa gctaaaatgt tcatgaaggg cgctctggtg gtagccccat
    18541 cggagcggtc aaaaaatgcc cagctgacgg gagaaattcg cggcgatctg gcgtggacag
    18601 gcaggacttt tcgcggcacg atcagcccct cctcggtggc atcgtttacg gatttcaagc
    18661 aggcatcctt tcacgtcagc ggcggaagcg tgcaagtgga agaatcgctt cccatcgaag
    18721 tggacctctc gactggacgg tgggcttccg gtccgggcct ggtggcctgg aggtcgccat
    18781 ctatagagct gagcggctcg tccctgacga tgcagcgggc cctcacgagg gtggatcgaa
    18841 ttgaaggcgc gtcgatggtg cagcgagcgc agatgacagc gacgctcgaa gggcttgttc
    18901 ttgcgcagtc atctttccgg agcctgccag tggatgtgac ggtgcgcgtg caggccgatc
    18961 cctccggggt gaaagcagac atccacccca aaaaccaaca tcagggcatc aaactcacgg
    19021 ggcagctgga gcaccaatgg gcggagggcc acggcaccgt ccatgcggtg gtagggccgc
    19081 tggccttcaa tcgagagaca tttcgcctcg gacaactctg gactccctgg ccttatgcgg
    19141 ctgacgtcac cggcggtgaa ctgaccggga tcgtcgatat gcgatgggcc acggacagcc
    19201 gccagcgact gcaaatacag ggtggatcag tggacatcag cgctgacaga ctggcgggtc
    19261 gttaccgcga catgctgttc tccggattca gcaccaatgt caaggtcgcg ctcgaaggtc
    19321 tgcaacgcct ctcgacttcg aggccagccg aggtcaccgt ggggtcgctc cagacggggg
    19381 tggaggtaac cgatctcaaa atgacagtcg aaggagaatg ggatgtcagg gaacggcttc
    19441 cgctcatgga aatacgcaac attcgttgtg aggtgttggg cggaacggtg acaagccagg
    19501 gtttgcgtgc agacctcgcc catcctccct atgggcttac gatgctggcc agacaacttg
    19561 acctccaaaa gattttgacc ctggaacagc agaaagggtt gcaggggaca ggcgtgctcg
    19621 acggctccgt tcctgtgatg gtgacgtctc gagggctcac ggtgagggac ggaagttttg
    19681 aggcccgtcc gccaggaggt gtgatccgtt atctggcatc accgcaagcc acgcatgcag
    19741 tgacgcaagc caacgcgaac atggaggccg tgctccaggc actcaacaat tttcagtaca
    19801 atgtgttaca agttggtgcc gaatatatcg aggatggcac attacagttg aaggcgcggc
    19861 tcgaaggcag gaatcccgat cagaaaaaaa gcccgcctat tcatttcaat ctcacggtgc
    19921 aggaaaatat cccggcgtta ttgaagagtc ttcgacttgt gaatggtgtg gaagactcga
    19981 tgcggaaagc attctcggga tctcaacgat aaggggggag gatggttcgc gtcgtctcca
    20041 tggtgacggg aggggtggtc ggcctgtatc tcacggtcgg gggatgcacg ccgcgggtcg
    20101 aaatggcaac acccgacaaa ccgatcacga tcaatttgaa cgtcaagatc gatcatgaga
    20161 tccgcgtgaa ggtcgacaag gagctggatg aagttctctc tcaagacggc gggctgtttt
    20221 aggttgtaga ggaggccgtg atgaggaata cgcatatcgg ccggagagtc gcctggcttg
    20281 tcttcttctt gttagcagtt tctccggcgt ttggcatctc cgtggaagaa gcaaaaacaa
    20341 aagggttggt gggtgaaagg ccgaacgggt acttgggcgt cgtgaatccc gccgctcccg
    20401 aggcgcaggc attggcgaac gaggtgaatg aaaagcggcg ccaggcgtat gaggacatcg
    20461 ccaagcggaa ccgaacgcag ctcgatgccg tcgaggcgct tgcaggagag aaagcgattc
    20521 aaaacacgaa gcccggctat ttcgtagaag gaccgggtgg gtggacaaga aagtaggttt
    20581 ggctgctttt cacacagcat ggctgacacc aagaggtcga gccgctgcgt tgcctcgtgt
    20641 aagcctgtgc gcgatgtgag aaggaagctg acagcctgct tcagagatta cccgtagcga
    20701 tcattgtccc atggcaacgc gctgttcgaa tagcctcgta cttcccaaaa tcccttttca
    20761 tctttgtcgg agaacgtgat ttcctttatc cacttcgcgc ctttccatgc gtatcgtttt
    20821 ggcacgatca tgcggaccgg gccaccgtgc tctttggcga gcggtttgcc gttccatttg
    20881 taggtcaaca gaacgtcatc gtcgtcacat gcttcgagcg gtagattcgt cgtgtaatcg
    20941 tcatacgact taaacaggac gaacttggcg agcgagagtg gtctcaccac cgacaggatg
    21001 tgcttgaaac tgaccccttc ccattggttg tcgtaacggc tccatgacgt gacacagtgg
    21061 aaatcagaaa catccttgaa ctgcggctgt gcgagaaact gctcccatgt ccatgtcagg
    21121 ggggacgcga caaagccacc gattgtgatc gtccaggttg agaggggaat atccggttta
    21181 tagcccaaat ccagaacagg gaacgtctcg accacgtgct gtccgggcgg caatcgctcg
    21241 tcccctgcgt aaaatacttc gcgctcttct ccccctcgcc gcgcttttgc ccactgttcc
    21301 ttcgtcttgg tcaaacgtgt cggttcatcc attgtgtgcc tcctcccaca gggtaggaca
    21361 gggcggtagg gtctggcaag cggcggccat gggttgcggc tcgacaatcg gcaggcgtgt
    21421 aaatcattgt gatcacagag aatcctactt acctgctaca cttaagggca ttgtcgcagg
    21481 ggagccttat gaagacatgt ctgcttgttc tagtcataac cggatcctgg tggctgtgca
    21541 tgacgattgt cgccatggaa gagacccagg cagaacaaag ggttcgtaag gagggatcct
    21601 catccggcat gccggcaaaa agtctcagac cggtcgaagg aaggcagaag caggcggaga
    21661 ccaagacgct ttcttctgtt aaaccggctg ccggtcggaa accgcaccgc gtcatcaaga
    21721 gctccaagaa agtgccgcgc aaaatcctgg tggcgcagcc acgaacggat ctcattcatt
    21781 atggtgttct cgaagattcg cagcgatatg atccgcgtcc ccatgctcat gctgccggtg
    21841 tgccccatcc ccagacgccg gacctcacgc atgatcattt ccaagaactg gaccgcaacc
    21901 aggatggcaa aatagacccg gttgaacggg cgttcggccg tattgacatg gaccgagacc
    21961 ttaatacccg tacgtttaga tagtttcctc cagccattct cattctgctc actcagccct
    22021 tatcgggagt ctccgcgttg agcctgccgg ccggtcggca caattatcat cgtctatcga
    22081 tggtcggttg ggcgttttcg ggcgagccat ccacattgac agttggaaga ggagtcccca
    22141 tcattagccc gcacgtccac cccgggaacg tgatcgatga atgcaacgac attgccgccc
    22201 cgtgccctac gagaagcggg caaacaaatt tcaaatgcgt catcttgatc ggccgtcgtc
    22261 ctgagaggag caggaatgtc tgatatggag ttgagtggac tactgcccga cgatcaagac
    22321 gatcggatcc ttgtggagaa tgtgcacccg acgaaatggg taaaccccgc tgccaacggg
    22381 cgttacaaca tcgtcgtact cggcgcggga accgccggct tgattaccgc gatcgtgggc
    22441 gccagcttgg gcgccaaagt tgcgctggtc gaaaaacatc tcatgggcgg cgactgtttg
    22501 aatgtggggt gtgtcccgtc aaagagtgtt atccgtgccg cccgtgcatg ggcggatctt
    22561 cgcaacgccg agcagttcgg actccgcatt cctgccgggg tgcagtacga cttcggtgcg
    22621 gtcatggccc ggatgagaaa actacgggcg cggatcagtc acaatgattc agcccagcgt
    22681 tacacgaacc tgggggtgga cgtgttcatc ggcagcggac gatttgccag tgccgatacc
    22741 attcaggttg aaggagtggc tggaaatcgc actctacggt ttgctcgggc cgcgatttgt
    22801 accggtacgc gcgcctcatc gcctgccata cctggactaa aagaagcggg ctacctcact
    22861 aacgaaacag tgttcgcctt gacggaattg ccgcaacgta tcggtgtgat cggagccggc
    22921 cccatcggct gcgagctggc gcaggccttt gcgcgttttg ggagtcatgt ctatgtgatc
    22981 gaagcgctgc acggcatcct ctccaacgaa gaccgcgatg cggctcaagt ggtcgagcaa
    23041 cagatgatga aggacggcgt gaagctgctc tgttgtggaa gggaccttca catagtccaa
    23101 acggcggacg gcaagaagct gtcggtccag tctcatggcc aacagtacga cataacagtg
    23161 gatgagattc tcgtggcggt cggacgcacg ccaaatgtcg agggtttggg cctcgaagag
    23221 gccggtgtgc aattcgatcg acgcggggtg caggtcaacg gccggttgca gaccaccaac
    23281 ccaaagatct ttgccgccgg cgatgtctgc tcgccgtaca aatacaccca tgctgccgac
    23341 gcaatggctc aaattgtcgt tcagaatgcg ttgtttccgc accccttcag cctcgggtat
    23401 gccagcatcg attcgctgat catgccgtgg tgcaccttca cggaccctga gattgcccac
    23461 gtcgggctgt atgaaaagga tgcggaagcc aaggggatcg gcgtggaaac gtacacgttc
    23521 aagctcgacg aagtggatcg tgccatcctt gacggccaag aggaagggtt cgcccgggtg
    23581 cacatccgaa agggcagcga caaaatcctg ggcgcgacga tcgtggctgc ccatgccgga
    23641 gaccttatca atgagttttc ggtaatcatg aaggcaggca agggcgcgag gactatcgcg
    23701 ggcaccatcc acccttatcc gactcaagcc gaagtcaaca agaaggtcat caatctgtgg
    23761 aggaaggcac atttcacccc ggccacgaaa cacaagctga tgcggctgtt cgcatggatg
    23821 cggcgcggat aggaccacat gagaatgttt cacccaggga ccccttggct cgccgcggtg
    23881 tggctcttgt cctcttcggc atcggcggcg accgagcctc ctcccgtgct cgaagttgga
    23941 aggtttgcct caagtcacgc cggtgcgccg ctggcggatg gctggaaacc gctcagattt
    24001 aagaaagtcc cgaaccacac ggtctatgag ctagtggaag acgcaggggc ggtggtcgta
    24061 cgggcgacga gcgaggcgtc ggcctcaggc ctgatcaaag agatcacaat cgatccttcg
    24121 gcatttcccg tcgtgcgttg gcggtggaag atcgagaatc ttctcaagca cagtgatgtg
    24181 cgccggaagt ccggggacga ctatccggcc aggctgtacg tgacgttcga atatgacccg
    24241 gacaaagtca gctttcccaa gaagctcaag tataaagccg gacaagcgct ttttggcgac
    24301 attcctatcg cggccctcaa ttacatttgg gacacgaaag cgccgatcgg aacggtcgtc
    24361 gacaatgctt tcaccgattt cgccaagatg atcgtggtgg agagcgggcc ggcaaaggtc
    24421 ggcctgtggg tcgaagaatc acgcaacatc tacgaggact acaaggcagc gtttggagaa
    24481 gaaccgccga agatcaacgg tgtggcgatc atgagcgata ccgacaatac caaagagcga
    24541 acgatcgcgt attacggcaa tattcagttt gtacctgccg gccaatgaag gagagaaagt
    24601 cctgagacgt gaaaaggccg acctattcag gtcggcctct tcgcagacac caccacgccg
    24661 ggagcgggtg cgcggattcg aaagagatta ctggatggaa ttcgcgaagc cgccccgcgt
    24721 aatctcagcg cggacggtgt ctccgacctt cttgttcttg atgtgctttg tgccctgccc
    24781 aacgtacagc ttcacctggt tgccttcata atcctcgacc gtgtaggcat tgtcttggat
    24841 atccttgacg gtgccgccga cagtcttcca ggcgggccct ggcttttcat cgtgatggcc
    24901 gcgcttggga gcttccgaga cttgcggcac atgtcccatt gacgctgccg cggcagcggc
    24961 tgacttgtgg gagggcggcg ccttcttctt cgaatctttg gccagggccg gtgcgacggc
    25021 gatggccagt atcgagacca tagcgattgc gattgggaat attgtcttcc tctttgcgtt
    25081 catggaaacc tccttaagac gacatggggt catagccttg cctgaccctt tcagcaaggc
    25141 atgtgcccgc tggtagcgct agcaattcat ggaaattcag taggttgtag atcctggtgc
    25201 tcccgcggcc ggtcaccctg tcacgaatgg aaaaaaaacg gcagaaatct gtccaaatat
    25261 gaccctctgt gccgatgtga ggaacagtga tggcagggcg aatgatcggg catctaccgg
    25321 ccaggtcgcc gtttcgagac atgtaggaaa ccgctacaat gcgcgagata tgccgcggag
    25381 agatccagac catcttctga gccgattgcc tcgaaaggcc tctctattgc tgcgcgatct
    25441 cgggagaatg gcggacgaac gagaagtggg tctgtatctt gtcggcggtg tcgtgaggga
    25501 tctcttgctc aaacgaagca attgggatct cgacctgacc gtggagggtg acggcattgc
    25561 atttgctcgc ctcgtggcca gccgctacgg cgccggtgtg gctctgttcg agcgcttcgc
    25621 gaccgcacgg ttgacgatgt ccgaccacac caaggtggac attgctagca cccggcggga
    25681 gtcctacgcc gatccggcag ccttgccgga agtgcgtcca gctcgcctcg aagaagatct
    25741 cttccgccgc gacttcacaa tcaatgccat ggcgatcgag ttgaacgacg cacagtgggg
    25801 acgcctacac gatccgaacg gcgggcggga ggatctcaaa accaaaatca ttcgagtgct
    25861 gcatgccaaa agttttgtgg atgatccaac aaggattttt cgggccattc gtttcgcaga
    25921 acgattcggc tttcgtttgg aggccacgac tcaccgtctc ttgaagcagg ctgcagagac
    25981 caacatcgtc gctcagcttt ctggtccccg cctggccaac gagatcttca tgctcatgag
    26041 ggaacgccat cccgaac
//
LOCUS       DASSCQ010000206         8251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_245039, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000206 DASSCQ010000000
VERSION     DASSCQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245039"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFH05_13130"
     CDS             complement(<1..199)
                     /locus_tag="VFH05_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679213.1"
                     /translation="MLVEAMQSSYPYFAHQHRFRGYAGGWDHCVLVQKLLSSPTPASK
                     RRDASTGPVSDPAVAIIPLWHV"
     gene            complement(350..1930)
                     /locus_tag="VFH05_13135"
     CDS             complement(350..1930)
                     /locus_tag="VFH05_13135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET6679214.1"
                     /translation="MRRIVWLGSWLLPLLSLTACSNLFPTLDLSPPYQPPEYVVPASW
                     HGSSPFIEANPSDGDLRPDWWTLYDDPALNTLIEQAMAANPDLEAAAERFVQARDMMM
                     KARSQYLPRLGLGFGASDNRASVNALFFPRDISQEASSVSLGGLASWEPDFWSALRNA
                     TRIETYRAEERAADYGLARLSLQAEIGANYFLLRGYDAQIAIYTQSIDLYRRSLDIVK
                     AQFAGAIASALDVARVESLLFTTETQLARVQGQRQVTEQAIAILVNMAPASFTIKPKA
                     AEDLRVASFALPRTLPSTLLERRPDIARMERQMAQASRAIGIARAAFFPDVRFRAGGG
                     FEDAGFNLLSLANSFWSYGAAVSMPLFQGGFRRAQLQQSWSAYRETEDRYRSTVLNAF
                     REVENNLSLTSQLTLAANRQDAAVGATFKAQHLTTELYQGGLASSLELIYAQVATLTA
                     RIESVQIKAELLQSSVGLIRALGGGWNRSQLPSDEQIQPFGTFQYVNLDKPAPIKGID
                     VNAPNNSVNNNLTKPFLP"
     gene            complement(1945..3186)
                     /locus_tag="VFH05_13140"
     CDS             complement(1945..3186)
                     /locus_tag="VFH05_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6679215.1"
                     /translation="MMRRLRDKFVALIMLIVFLFYVGYRIYETKSDAAALQVKTLEDA
                     VPTVAIVHPKPAEASETIILPGNITAWFEAPIYAQVSGYVKMWYKDYGAQVKKGDILA
                     EINAPALDAQYAQAKADLESERAKYALADITAKRWLALRPNQAVSEQSISVKVAEAKA
                     EAAKVKASEQNVRNYEALIRFKTIVAPYDGVVTVRNINVGDYVNKEGTISTPGSISNL
                     FTVADVSMMRLFVSVPESFGPFLNPGLTADVTVPQLPDRHFTGKFLTVAKGFDVSTRT
                     AVTVFTIDNDDKALWPGSYGKVVITAPVDRKVFLIPSTALVFQEHGTQVAVVTDEDRI
                     HLQTITVSRLLDSAVEVSEGIGASDRIVNNPSAALLEGDKVRIVTPAPGYDLDQTEPS
                     SPVDQSQQHAQTKQSALSTSR"
     gene            complement(3183..4868)
                     /locus_tag="VFH05_13145"
     CDS             complement(3183..4868)
                     /locus_tag="VFH05_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6679216.1"
                     /translation="MSQQPDQHMQPSRNVFVRFQKGFERRFDRFREGYGALLERVIAH
                     RSAFITISLTFAVGSTVLFYFLGRDYFPEIRSGVIQMHMRAPLGTRIEVAGRLATLVS
                     NSVEELLPGQVENIVSNCGLPVGPHNLAFIPTPTIGSQDCDLTILLENEKSPVWDYRR
                     ILRKGLRERYPGTEFTFQPSDLTAKILNFGAPAPIDVQVNGPDIYANYEFVRKLVGKF
                     RDVPGAVDVVIQQTMRTPTLMVEGNRTFGLGVNRTLKDMADNLLMTTAGSQQVDQIYW
                     LDPSTGMSYLINVYTPQPQINSLNSLKTIPIDSSANPSKDNDIQLLGNLADLSAEGTP
                     GVITHGNIMPLFDIYISAEGRDLGGVLTDVQEIARGMESEKPRSAELEIHGQASLMHE
                     AYSELIFGLLAAIVLVYLLIVVNFQSWLDPFIIITALPGALAGIAWALFLTHTRLSEP
                     ALTGAILTMGTATANSILVVSYARERIQEHGDALRAAIEAGKTRFRPVIMTASAMIIG
                     MVPMATGYSQNAPLGRAVIGGLFVATVFTLLFVPCVYAMFYNRRMARQKERAS"
     gene            complement(4852..6351)
                     /locus_tag="VFH05_13150"
     CDS             complement(4852..6351)
                     /locus_tag="VFH05_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6679217.1"
                     /translation="MNQIVLIALRRPYTFVVMSILILVLGTKTVLKMPTDVFPNITIP
                     VTSVVWIYTGLLPQQVEGRITYLFERFLTSTVEGIKYIHSHSYYGSSITNIFLQDGVD
                     VGRAEADIVGIAQNVVKALPPDISPPMVMRLAPSSIPVAMIQISSDNMTPAELYNLAY
                     MRIRPLLVTVPGIVLPHPYGGQDMQVMVNLDQQKLLARHLTPTDIHKVLMQQYLVLPA
                     GDIKIKSTDWIVLTNASPMKIEDFGEIPIKREGNAFIYLRDVATVQLMGRVQQNAVLV
                     KGKQTVIIVAMKSTEASTLDVVDGIKKMIPRAEQVSPPGVKIKLLDDASTFVKDSISD
                     VVHEMATAGALVGLIVLLLLGSWRATVIVWTSIPLSILTAIIGLHWLNETINVMTLGG
                     LALAVGILVDDATVMIENIDRHLEMGKPLEQAIIDAANQIVVPTLVATLCIAIVWIPL
                     FELGGVAGYLFKPMAEAVIIAMLASFILSRTLVPTMAKYLMKSHHVAAA"
     gene            6737..7537
                     /gene="modA"
                     /locus_tag="VFH05_13155"
     CDS             6737..7537
                     /gene="modA"
                     /locus_tag="VFH05_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6679218.1"
                     /translation="MMVRMRKFIIAVILAGAMTGVGMGVPASGETLTVAATPSLRAPF
                     QEILRMFEQEYGVSVEVRYGPSHTLRRQIEQGAPIDVFLSESWQEVETLHQKELTLFG
                     RPLQYAQTSMVVVMSSASTATVVSVSEASSHGVLRVALVDPKASSVGAIAAKALKEMD
                     PVAYHHARLIPAPNTDEVVRLVSAGEADVGIVYRADAINSGLVRIIDEAPAGRAIPVV
                     FGQAVVSTCREASRQAAQQFTNFMMSPRIQKLLLKYGFDAVPTKAAVG"
     gene            7700..8218
                     /locus_tag="VFH05_13160"
     CDS             7700..8218
                     /locus_tag="VFH05_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679219.1"
                     /translation="MAFLPFRDRTRMMQFRWPAIAAGLSVALGIHIVLTAFGLGVVFL
                     QSQSAEGASSTATAALLLWSGLSWVMAGFIGGYITAWVADASRYVESLFHGLVLWGAL
                     TFILMFLPPSTMGITSEAEPALSPSTISSVAWFVAIGGLLSLGTTIWGAVIGSRVVAE
                     VETKQTGTYRAA"
BASE COUNT         1737 a         2247 c         2497 g         1770 t
ORIGIN      
        1 atacatgcca aaggggaatg atcgccaccg ctgggtcgga gacaggtccg gtcgatgcgt
       61 cgcggcgttt cgaggccggc gtaggtgacg acagcaactt ctggaccagg acgcagtgat
      121 cccatccgcc cgcatagccg cggaacctgt gttgatgtgc aaaataggga tacgacgact
      181 gcatggcttc aacgagcatg tttgaattcg acctgagcgg caggtgcgga ttcgctcagt
      241 cgaaaacatt tcactcgacg atgtgatcag atgccgtggg tcgaggacct tctgcaagac
      301 aatccgggga cagggaaagt gaagaatttg gtgtggagcg cttcttcgct cagggaagga
      361 aaggtttcgt cagattatta ttgaccgagt tattcggcgc gttgacatcg atgcccttga
      421 tgggagccgg cttatcgaga ttgacgtact ggaacgtccc gaacggctgg atttgttcgt
      481 ccgagggcaa ttgacttcgg ttccagcctc cgccgagcgc acgaatcagt cccaccgagc
      541 tttgcagcag ctcagccttg atctgcacgg actcgatgcg ggcggtcaac gtggcgactt
      601 gcgcataaat gagttcaagg ctggatgcca gaccgccctg atagagctcc gttgtcaggt
      661 gttgcgcttt gaaggtggcg ccgaccgcgg catcctgacg attcgcggcg agggtcaact
      721 ggctcgtcaa actcaggttg ttttcgactt cgcgaaaggc gttgagcacg gtcgaccggt
      781 atcgatcttc cgtctcgcgg tacgccgacc aggactgctg caattgcgct cgacgaaatc
      841 cgccctggaa gagcggcatc gagacagccg caccatagga ccaaaaactg ttcgccagag
      901 aaagcaggtt aaagccagcg tcttcaaagc cgccgccggc cctgaacctc acatcgggaa
      961 aaaaagcggc cctcgcgatg ccgatggccc ggctcgcctg cgccatctga cgctccatcc
     1021 tggcgatgtc cggtcggcgt tccagcaacg tcgaaggcag cgtccgcgga agggcgaaac
     1081 tcgccacgcg cagatcctcg gctgccttgg gtttgatggt aaaactggcc ggcgccatat
     1141 tgacaaggat ggcgattgct tgttcggtga cttgtcgttg tccttgtacc cgtgcgagct
     1201 gcgtctcggt ggtaaacaac agagattcga cacgagccac gtcgagcgcc gaggcgatcg
     1261 cgccggcgaa ttgggccttg acgatgtcca acgaccgtcg atacaggtcg atcgattggg
     1321 tataaatcgc gatttgcgcg tcatagcctc gcagcagaaa ataattcgcc ccgatttccg
     1381 cttggaggct gagacgtgcc agtccatagt cggcggcgcg ctcctcagcg cgataggtct
     1441 caatgcgagt ggcatttcga agggccgacc agaagtcggg ttcccaggag gccagcccac
     1501 caagggaaac cgatgaggcc tcctgcgaaa tatcgcgtgg gaaaaacagc gcattgacag
     1561 aggctcgatt gtccgacgca ccgaacccga gacccaggcg cggaaggtac tgagaccggg
     1621 ccttcatcat catgtcccgg gcctgcacaa accgctcggc ggccgcctcc aaatcaggat
     1681 tggccgccat ggcttgctca atgagagtat tcagggccgg atcgtcatac agtgtccacc
     1741 aatcagggcg caggtcgccg tccgatgggt tggcctcgat gaatgggctc gatccatgcc
     1801 aggaggcagg gacaacatat tcaggcggct gataaggcgg cgagagatcc agcgtcggaa
     1861 acagattgct gcatgcagtc aggctgagaa gaggcagaag ccagctcccc agccagacga
     1921 tacgcctcat atgcgtgaac cccttcatcg tgacgtcgac agggccgatt gctttgtctg
     1981 cgcatgctgc tgcgattgat ccaccggcga ggaaggctct gtctggtcga ggtcatagcc
     2041 cggcgccggc gtgacgatgc gaactttgtc gccttccagc aatgccgcac tgggattgtt
     2101 cacgatgcgg tcgctcgcgc caatcccttc agacacttcg accgcgctgt cgaggagtct
     2161 gctcacggtg atggtctgca agtggatccg gtcctcgtct gtgaccacag ccacctgtgt
     2221 gccgtgctcc tggaagacca gcgcggtaga gggaatcaag aacactttcc gatccaccgg
     2281 tgccgtgatg acgactttac cgtacgagcc aggccacagg gctttgtcat cgttgtcgat
     2341 ggtgaagacc gtcacggcgg tgcgggtact cacgtcgaac cccttggcga ccgtcaaaaa
     2401 cttgccggtg aaatggcgat cgggcagttg gggaacggtc acatcggcgg tcaggcccgg
     2461 attgagaaac ggcccaaacg actcggggac actgacgaag aggcgcatca tgctcacgtc
     2521 ggcgacggta aagaggttgc tgatcgagcc gggagtactg atcgtgcctt ccttattgac
     2581 gtagtcgccg acgttgatgt tgcgcacggt caccacgccg tcatagggcg caacgatcgt
     2641 tttgaatcga atcagggcct cgtagtttcg gacgttctgt tccgacgctt tgaccttcgc
     2701 cgcctctgct ttcgcctcgg cgactttcac cgagatcgat tgctcggaca ctgcttggtt
     2761 agggcgtagc gcgagccagc gtttggccgt aatatccgcg agcgcatact tggcgcgctc
     2821 cgactcgaga tccgccttgg cttgtgcata ttgcgcatca agcgccggcg cattgatttc
     2881 ggcgagaatg tcgccctttt ttacctgggc gccgtagtct ttgtaccaca tcttcacgta
     2941 gccagagacc tgggcataaa tcggcgcttc gaaccaggcg gtgatgttgc cggggagaat
     3001 gatggtctcg ctggcctcag ccggcttcgg atggacaata gccacggtag gtacggcgtc
     3061 ttcgagcgtc ttcacctgca atgcggcagc gtcgcttttg gtttcataga tgcgatagcc
     3121 aacgtagaag aggaacacaa tcagcatgat gagggccacg aatttgtctc ggagacggcg
     3181 catcatgagg cacgctcctt ttggcgagcc attcgcctgt tgtaaaacat ggcgtagaca
     3241 caggggacga acaggagtgt gaagacggtg gcgacgaaca atccgccgat gacggcgcgg
     3301 ccgagtggcg cattctgaga atagccagtt gccatgggga ccattccgat gatcatggca
     3361 gaggcggtca tgatgaccgg gcgaaagcgg gtcttaccgg cttcaatcgc cgctcgcagt
     3421 gcgtctccgt gctcctgaat tcgttcgcgg gcataggaca cgacgagaat cgaatttgcc
     3481 gttgccgtgc ccatggtcag gatggcgcca gtcagtgccg gctcggacag acgcgtatgg
     3541 gtcagaaata atgcccacgc gatgcccgcc agcgcgcccg gcagcgctgt aatgataatg
     3601 aacggatcga gccacgattg aaagttgact acgatcagta gatataccaa gacgatggca
     3661 gccagcagtc cgaagatcag ctccgaatag gcttcgtgca tgagagaagc ctggccatgg
     3721 atttcgagct cggcgctgcg gggcttctca gattccatgc ctctcgcgat ctcttgaacg
     3781 tctgtcagca caccgccgag gtcgcgtcct tcggcggata tataaatatc aaagagaggc
     3841 atgatgttcc cgtgtgtgat cacccccggc gtgccttcgg ctgacaggtc ggccaaattg
     3901 ccgagcagtt ggatgtcatt atctttggaa ggattggccg aggaatcgat cggaatggtc
     3961 ttcagactat tcaagctatt aatctgcggc tgcggcgtat acacattgat cagataggac
     4021 atgccggttg agggatcgag ccaatagatc tggtcgacct gttggcttcc ggcagtcgtc
     4081 atcagcagat tgtcggccat gtccttcagg gttctattca caccgagtcc gaaggttcgg
     4141 ttaccttcga ccatgagggt cggcgtgcgc atggtctgct ggatgaccac atccacggcg
     4201 ccggggacgt cgcggaactt gcccaccaat ttgcggacga actcgtaatt ggcatagatg
     4261 tcggggccgt tcacctgcac gtcgatcggc gccggtgcgc cgaagttgag tattttggca
     4321 gtcaaatccg atggctggaa cgtaaattcg gttccggggt accgctccct caagcccttg
     4381 cgcaagatgc gccggtaatc ccacaccgga gacttctcat tctcgaggag gatcgtgagg
     4441 tcgcaatcct gagagccgat cgttggagtc gggatgaagg cgaggttgtg cgggccgacc
     4501 gggaggccac aattactgac gatgttttcg acctggccgg gcaacaactc ctcgacgctg
     4561 ttagagacga gagtggccag gcggcctgcg acttctatgc gcgttccgag cggcgcccgc
     4621 atatgcattt gaatgactcc ggaccggatc tcggggaagt aatcgcgtcc gagaaaatag
     4681 aagaggactg tggatcccac cgcgaacgtc agcgagattg tgataaaggc gctgcgatgg
     4741 gcgatcacgc gttcgagcag tgcaccgtag ccctcgcgga atcgatcgaa acggcgttcg
     4801 aaccccttct ggaaacggac gaagacgttc cgcgaaggct gcatgtgctg gtcaggctgc
     4861 tgcgacatgg tgactcttca tgaggtattt cgccatcgtc ggcaccaaag tgcgcgatag
     4921 aataaacgag gccagcatgg cgatgatgac tgcctcggcc ataggtttga acaagtaccc
     4981 cgcgacgcca ccaagctcga agagcgggat ccagacgatc gcgatgcaga gggtcgcgac
     5041 gagagtggga acaacgatct gattcgcggc gtcgataata gcctgttcca gcggcttgcc
     5101 catttccaga tggcgatcga tattctcaat catgacagtt gcatcgtcga ccagaatacc
     5161 cacagcgagc gccaagccac cgagcgtcat gacattgatc gtttcgttaa gccaatggag
     5221 accgatgatg gcggtgagga tcgacagcgg aatggacgtc catacgatca ccgtcgccct
     5281 ccacgagccg agcagcagca ggacgatgag gccgacgagc gctcccgcgg tggccatttc
     5341 atgcacgacg tccgagatcg aatccttgac gaaggtcgaa gcgtcgtcga ggagcttgat
     5401 ctttacgccg ggaggagaca cttgttcggc ccgcgggatc atcttcttga tcccatcgac
     5461 tacatccaag gtcgaggcct cggtgctctt catggcaacg atgatgacgg tctgtttgcc
     5521 cttgaccagc actgcgttct gttgcactcg gcccatcagt tgcaccgtgg cgacgtcgcg
     5581 cagatagatg aacgcattgc cttccctctt gatcgggatc tcgccgaaat cctcgatctt
     5641 cattggtgag gcgttcgtca gcacgatcca gtcggtggat ttgattttaa tgtcgccagc
     5701 aggcagcacg agatactgct gcatgaggac cttgtgaata tccgtcggag tcagatgacg
     5761 cgcgaggagt ttctgctgat cgaggttgac cattacctgc atatcctgac ctccgtacgg
     5821 gtgcggcaac acgatcccgg gtacggtcac gagcaagggt cggatccgca tgtaggccag
     5881 attgtagagc tccgccggcg tcatattgtc ggagctgatt tggatcatgg ccaccggtat
     5941 tgaggatggc gcgagacgca tgaccatcgg aggggaaata tcgggcggca gcgccttcac
     6001 gacgttctgc gcaataccca cgatatccgc ttcggccctg cccacgtcca cgccatcctg
     6061 cagaaaaata ttggtgatgc tgctgccgta atacgagtga ctgtggatat acttgatgcc
     6121 ttccactgtc gaagtcagaa agcgttcaaa tagataggtg atgcgccctt cgacctgctg
     6181 cggcaacagg ccggtgtaga tccagaccac ggaggtgaca ggaatggtga tgttcgggaa
     6241 gacgtcggtc ggcatcttga gtaccgtttt ggtacccagt accaggatca agatggacat
     6301 gacgacgaag gtgtagggcc ggcgaagcgc aatgagaacg atctgattca taggccgggc
     6361 gaactccggt cagaggcaac tctagtttag tgcaacgtgc ttcggcgtag caaacggctt
     6421 acctgtctcc taccgcccgc ttcccgaggt gtatgtttcc tgaatgagcg ccatttatcc
     6481 gctggatatc tccagaaccc tgatcgaatt gtttgcgaat gaaacaggaa cagccagaga
     6541 ggtggcgttt tcgcacagtg acgactgtgc gaaatctcac aggtctgctg aggcgacggg
     6601 ccgatcttag tccggaacct tccaaaatct cgtgatttcg attcatgtct ctcggggagt
     6661 catttgtagc ttcgatggtc ccgatcttgc gttgcacact atcgtacgct cagccatgga
     6721 ctttacgaag aggagcatga tggtgcgaat gcggaaattc atcatcgcag tgattctcgc
     6781 gggtgcaatg acgggggttg gaatgggagt gcctgccagc ggtgagacgt tgacggtggc
     6841 tgctactccg agcttgagag cgccatttca agagatcttg cggatgtttg aacaggaata
     6901 cggcgtatcc gtcgaggttc ggtatggccc ctcacatacg cttcgccgac agatcgaaca
     6961 aggagctcct atcgatgtgt ttctgtctga atcctggcag gaggtagaga cgctacatca
     7021 gaaggaactg acactgtttg gacgtcctct tcaatatgcg cagacatcaa tggtcgtggt
     7081 catgtcctcc gcgtccactg ccaccgtggt ctcagtgagt gaggcatcgt ctcatggggt
     7141 cttgcgtgtc gctctcgtcg atcccaaggc gtcgagcgtg ggagcgattg cggcgaaggc
     7201 gctgaaagag atggatcccg tggcgtatca tcatgcgcgt cttatcccag cgcccaacac
     7261 cgatgaagtt gtccgtctgg tctctgcagg agaagccgat gtcggcatcg tctatcgggc
     7321 cgacgcgatc aacagcggac tggtgcgcat cattgatgaa gcgccagccg gacgcgctat
     7381 tccggtcgtg ttcggacaag cggtggtatc gacctgtcga gaagcctcac ggcaggcagc
     7441 acaacagttc acgaatttca tgatgagtcc tcgtatccag aagttgctgc tcaagtacgg
     7501 cttcgatgcg gtgccgacaa aagcagccgt gggctaacgg tcgcaaatac gcgctgtcca
     7561 gatgctgtca accggatcct tcacgctttt gtccctagga gaagtccggg gttcgctccg
     7621 atgtcgccta ggtgtcgccc cgaaagctat ggttctcccc atgcgacctc caaaccgctg
     7681 acaggatatc gtgggaacca tggctttctt gccgtttcgt gatcgaacga ggatgatgca
     7741 attccggtgg ccggcgatcg ccgcaggact ttccgtggcc ttgggaatcc atatcgttct
     7801 cacggcgttt ggcttagggg tggtatttct ccaaagtcag tctgcggaag gcgctagcag
     7861 cacggccacg gcggcgttgc tgttgtggag cgggctgtct tgggtcatgg cgggattcat
     7921 cggcggttat attaccgcct gggtcgccga tgcctcacgc tatgtcgaaa gccttttcca
     7981 cggactggtg ctttggggcg cgttgacctt catcctgatg ttcttgccac cgagtaccat
     8041 gggcatcacc tctgaagcag agccggcctt gtcgccgagc accatctcat cagtggcctg
     8101 gtttgttgcc attgggggat tgctttcgct cggcaccacg atttggggtg ccgtgatcgg
     8161 ctcacgtgtg gttgccgagg tggaaacgaa acaaactggc acctaccggg ccgcataaga
     8221 atgacccatg gttcgtatct ttctcactgg a
//
LOCUS       DASSCQ010000207         5566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_263904, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000207 DASSCQ010000000
VERSION     DASSCQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263904"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFH05_13165"
     CDS             <1..695
                     /locus_tag="VFH05_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET6679220.1"
                     /translation="WSQGEAGSSFRHPVIVAPMPRVDFYRILGVSREASEDVIKKAYR
                     KLVFEHHPDRNPHSKQAEEKIREINAAYEVIGDAEARRNYDRLNWGDEFAPDVVDVAV
                     LLEEMERKLFDEGRKELFALLVKDVTTIKAELALIRDRVVKDQGYDSFKETLVHQRAA
                     EVMPDLVTDEMNGRKTRLVEVATQMMVSQGVVARNDEGGIRSLRSRLDEQFNRGRLHG
                     FASALELFYERR"
     gene            complement(716..1927)
                     /locus_tag="VFH05_13170"
     CDS             complement(716..1927)
                     /locus_tag="VFH05_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6679221.1"
                     /translation="MAIAIHNVRLLDGSGHAIERATVIVQEKRIHAAGPSRTVNIPRG
                     ASRIDGRGLTLLPGLIDCHVHFSLGAEPDVVKAIELETPSETLLKAAQLARQTLEGGV
                     TTVRDVGSRDHSIFTLKRAIDKGLTEGPRIIGAGLAICMIGGHARFIGQEVEGVEQVR
                     KVVREQIDAGAQVIKVIASGGVLTPGTSPDQAQMTMEELRTAVDEATRAGRKVAAHAH
                     GAAGMKNAIRAGVHSIEHATLMDQEAAALMLEHGVYMVPTLSALATTAACRRGCGIPD
                     SALEKAKTMTKRHETSFRQAHARGLLIAMGTDAGTPFNRHGENAQELERMVAFGMSPM
                     EAIIASTSAAARLIGVDDQVGTIARGKEADLLLIEGNPLRKVELLRDRSRIAGVMKAG
                     RFVAGPLRRPA"
     gene            complement(1932..2528)
                     /locus_tag="VFH05_13175"
     CDS             complement(1932..2528)
                     /locus_tag="VFH05_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET6679222.1"
                     /translation="MDNKPLLWVLGGGAFAFTAVVAYWIFAITLANHLKSDQVSPQKT
                     AEFVHAILEANRNNYTDNVVVKLSRDGIEAHEHWRDERGVPLPAQFLLESGRLVAVKD
                     LGFSFRLASLTPIYAWNGANSDLERVGLAAVQQDPSQPHYGIVRRGELRLFQAVYADR
                     AVSQACVDCHNRHPNSPRRDFKLNDVMGGIIITFPIGS"
     gene            complement(2536..3729)
                     /locus_tag="VFH05_13180"
     CDS             complement(2536..3729)
                     /locus_tag="VFH05_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET6679223.1"
                     /translation="MTIPEFTPPRRLLLGPGPSLVHPRVLRALSSPLLGHLDPVFLSV
                     MNDIQVSLRAVFQTANRFTIAISGTGSAGMEASIVNVVEPGDCVVVGINGVFGNRIAS
                     VVERCGGKVIRVEAAWGTCIEPDAIEQALVRSGPVKAVAIVHAETSTGAWQPLEDIGA
                     LCRRHEALFIVDAVTSLGGIPVEVDRVGIDVCYSGSQKCLSCPPGLSPFTMNDRAVGV
                     MKSRRSQCPSWYLDMGLIDAYWSEGLRVYHHTPPVSMLYALREALRLVEEEGLPARYT
                     RHQMNSDALVAGLKELGLVPLPAGGRALPMLNCMTLPAHIPEADIRAKLLSYYGIDIG
                     AGLGPLKGKVWRIGLMGESSTEAHVLTLLNALESLFMEGGWLATPGVALQAASRMYSR
                     LPDRR"
     gene            3888..5387
                     /locus_tag="VFH05_13185"
     CDS             3888..5387
                     /locus_tag="VFH05_13185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6679224.1"
                     /translation="MNEPLAIVTRPLARSWWRETEAPPTIGQFPNHYSSSISWLAGIF
                     LVAAFTSVLLMASTTSKIDRIDAPAQALSLMVGRTMDAQEGLSRAPAWEQRMMRWVSG
                     DGEAERAQAMAWYQELAARSDDPAVPLQQAILQAESGQLSPALLSAHEWSTREEPLPA
                     YGDIFRAAYGGDRPPDRHAVEALQSRMADVLPVGWFYDRLMMRLAQRAGHTELAAVID
                     EELSLRGDRLYRYSRRLTVLELLVMLAGTLLLFLIWQKHRAGINVVRLHDPGLPPPWP
                     GRVGTAVLLRGGAIGAIIMAVCLLYLPPENASLRALAIPLTNVPVLALAYYHLFRPAG
                     MTFEEGFGLSLPWSHIGRLMTAVLAVVAAGLWGEWLMDRVAEQWHLTSHWTEWFDEDL
                     VWGSSSLTRVSLLESVILAPLFEELVFRGLLFAILRRKFRFLPAALMSASIFGLAHGY
                     GAVGLISVCWSGVLWAWIYEKTGSLAPGILAHAINNLLVCLAVMSLLRF"
BASE COUNT         1086 a         1618 c         1677 g         1185 t
ORIGIN      
        1 tgtggagtca aggtgaagct ggaagctctt ttcgacatcc tgttatagtc gcgcccatgc
       61 cgcgcgtgga tttctatcgg attctgggcg tgtcccggga ggcgtcggaa gacgtcatca
      121 agaaggccta ccggaaactg gtcttcgagc atcatcctga ccgcaatccg cacagcaagc
      181 aggcagagga gaagattcgc gagatcaatg ccgcctacga ggtcatcggt gatgcggagg
      241 cgcggcgcaa ctacgatcgg ctgaactggg gcgatgaatt cgcaccggat gtggtcgatg
      301 tcgccgtgct gctcgaggag atggaaagaa aactgttcga tgaaggacgg aaagaactct
      361 ttgcgctgct ggtgaaggat gtaacgacga tcaaggcgga attggcgctc atacgggatc
      421 gcgtggtcaa agatcaagga tacgattcat tcaaggagac gctcgtccac cagcgcgcgg
      481 cggaagtcat gccggacctg gtgactgatg aaatgaacgg ccggaaaaca cgcctggtgg
      541 aagtggccac tcaaatgatg gtgtcgcaag gtgtggtcgc tcgcaacgac gaaggcggca
      601 tccgatcgtt acgcagtcga ctcgacgagc aattcaacag agggcgcctg cacggctttg
      661 cgtcagcgtt ggaactgttc tatgaacggc gatagtgcaa tgaaattcca gcatctcagg
      721 caggccggcg gagcgggccg gccacgaatc gtccggcctt catcactccg gcaatgcgac
      781 ttcggtcgcg caacagttcc actttccgca gcggatttcc ttctatgagg agaagatctg
      841 cctccttccc tcgtgcgatc gttccgacct gatcgtccac gccgatcaat cgggcggcgg
      901 ccgacgttga ggcgatgatc gcttccatcg gactcatgcc gaacgcgacc attcgctcca
      961 gctcctgggc attctctccg tgcctattga agggtgttcc ggcatcggtg cccatggcga
     1021 tcagcagtcc acgcgcgtgc gcttgccgga agctggtttc gtgacgcttc gtcatggttt
     1081 tggccttctc cagagcacta tcagggatgc cgcaacctcg gcggcaggcg gccgtcgtgg
     1141 cgagcgcaga aagcgtcggg accatataca cgccatgctc cagcatgagc gcggcggctt
     1201 cctggtccat caacgtggca tgctcgatcg aatgcacacc ggcacggatc gcattcttca
     1261 tccccgctgc tccatgcgca tgcgccgcga cctttcgtcc agcacgggtc gcttcgtcca
     1321 cggcggtccg cagttcttcc atcgtcattt gcgcttgatc aggggaggtg ccgggagtga
     1381 ggacgccgcc tgaggcgatc actttgatga cctgggcccc ggcatcgatt tgctcgcgca
     1441 ccacttttcg gacttgttcg acgccttcca cttcctggcc gataaaccgt gcatggccgc
     1501 cgatcatgca gatcgcgagt ccggcgccta tgatgcgagg cccctcagtc aagcctttgt
     1561 cgatcgcccg tttgagcgta aaaatcgagt gatcacgcga gccgacatcg cgaacggtgg
     1621 tcaccccgcc ctcaagcgtt tgcctggcca actgagcggc cttgagcaat gtttcagacg
     1681 gtgtttccag ttcgatggcc ttgaccacgt caggttcggc acccagagaa aaatgaacat
     1741 ggcaatcgat taaacctgga aggagggtga gcccgcggcc atcaatgcgg gaggcgccgc
     1801 gcggaatgtt cacggtgcgg ctgggaccgg cggcatgaat ccgcttctcc tgcacgatga
     1861 ctgtagcccg ttcgatcgcg tgaccgcttc catcaagcag tcggacgttg tggatcgcta
     1921 tggccacagt gttagcttcc gatcggaaat gtgatgatga ttccacccat gacgtcgttg
     1981 agcttgaagt cccgccgggg gctgttggga tgtcggttgt ggcaatcgac gcaggcctgg
     2041 gaaacggcac gatcggcata gactgcttga aacagacgca gctccccgcg tcgcacgatc
     2101 ccgtagtgag gctgggaagg atcctgttga acggccgcta atccgacgcg ttccagatcg
     2161 ctattcgcgc cgttccaggc gtagattggc gtcaggctgg ccagacggaa ggaaaagcca
     2221 aggtctttga cggcgaccag ccgtccagat tccaagagga actgagccgg aagcgggact
     2281 ccacgttcgt ctctccagtg ttcgtgcgcc tcgattccgt cgcggctgag tttgaccacc
     2341 acgttgtctg tgtagttgtt gcgattggcc tccaggatcg catggacgaa ttcagcggtc
     2401 ttctgtggtg agacctggtc cgacttcagg tggttcgcca acgtgattgc gaagatccag
     2461 taggcgacga cggccgtgaa ggcaaaagcc ccgccgccaa gcacccaaag caagggtttg
     2521 ttatccattc gaacgttacc tccggtcagg aagacggcta tacattcgcg aggcggcttg
     2581 cagcgcgaca ccgggtgtgg caagccatcc tccttccatg aagagcgatt ccagggcatt
     2641 caaaagcgtg aggacatggg cctctgttga agattccccc atgagaccaa tacgccacac
     2701 cttgcccttc aatggaccga gacccgctcc gatgtcgatt ccatagtagg agagcaactt
     2761 cgcgcggata tccgcttcag ggatatgggc agggagcgtc atgcagttca acatgggcaa
     2821 cgctcgtccc cccgcaggaa gcggcaccaa tccgagttcc ttcagtccag ccaccagcgc
     2881 atcgctgttc atctgatgac gcgtgtagcg ggcgggtaag ccttcttcct cgacgagtcg
     2941 caaggcttca cgtagggcgt agagcattga aacaggcggg gtgtggtgat agaccctcag
     3001 gccctccgac cagtaggcgt cgatcaatcc catgtccaga taccaactag ggcactgact
     3061 ccgtcgcgat ttcatgacgc ccactgcccg gtcattcatc gtaaacggcg ataatccggg
     3121 cggacagctc aagcattttt gtgacccgct gtagcacaca tcgatgccga cccggtccac
     3181 ctcgacggga atcccgccaa gagacgtgac ggcatcgaca atgaacagcg cttcgtgacg
     3241 gcggcacagg gccccgatgt cctcgagtgg ctgccaggcc cctgttgacg tttccgcatg
     3301 cacaatcgcg accgccttga ctggtcccga gcgaaccagt gcctgttcga ttgcgtcggg
     3361 ctcgatgcag gtcccccacg ccgcttcaac tcgaatcact tttccgccgc atcgttcaac
     3421 cactgaggct atcctgtttc caaagacgcc gttgatgccg acgacgacgc agtccccggg
     3481 ctcgaccacg ttcacgatcg acgcttccat ccctgcagag ccggttcccg aaatagcgat
     3541 tgtgaagcgg ttggcggttt ggaacactgc gcgcaacgag acctgaatgt cgttcatgac
     3601 gctgagaaag accgggtcca ggtgccccaa cagcggtgat gacagcgcgc gcaagacgcg
     3661 gggatgcact aggctcgggc cggggccgag aaggaggcgt cgcggagggg taaattccgg
     3721 aatcgtcata tctggccgct tgatgggaag agcatcttgg ctgggcagta taacggactc
     3781 tcgcttcatt cagccagttg gcgttctact tgccgttcta caaggaggca gcggttactt
     3841 ttgaggggtt tctggctcaa aaatacacat gttatagtcc aggcgagatg aatgaaccgc
     3901 tggcaattgt gactcgccca ctcgctcgtt cttggtggcg ggaaaccgag gcaccgccca
     3961 ctatcggcca attccccaac cattattcct cctcgatcag ctggctggcc gggatttttc
     4021 tcgtcgctgc ctttacatcc gtcctcctca tggcttccac cacttcgaaa atcgatcgga
     4081 ttgacgcacc ggcacaggcg ctgagcttaa tggtcggccg tacgatggat gctcaggagg
     4141 ggctctccag ggctcctgcc tgggagcaac ggatgatgcg ctgggtgtcg ggcgatggag
     4201 aagccgagcg ggcacaggct atggcgtggt accaggaact ggctgctcgc tccgacgatc
     4261 cggccgttcc cttgcagcag gctattctgc aagcggaaag cggacagctc tccccggctc
     4321 tcctgtcagc acacgagtgg agcactcgcg aagaaccgct tcccgcctac ggggacatct
     4381 ttcgagcggc ctatggcggg gatcgccctc cagatcgtca tgccgtcgag gcgctgcaaa
     4441 gccgcatggc ggatgtgctg cctgtcggat ggttttacga tcgtctgatg atgagattag
     4501 cccaacgagc ggggcacacg gagttggcag ccgtcataga cgaagagctt tccttgcgcg
     4561 gcgatcgctt gtatcgctac tcccgcagat tgaccgtgct ggagttgctg gtcatgcttg
     4621 caggaaccct cttactcttc ctgatctggc agaagcaccg cgcaggaatc aacgtcgtcc
     4681 ggttgcacga cccgggcctc ccgcctccgt ggccgggccg ggttggcacg gctgtcttgc
     4741 tgcgcggcgg cgcaatcggc gcgataatca tggcggtgtg tctcctgtat ctgcctccag
     4801 agaatgcgtc tttgcgagct ttagccatcc cattgacgaa tgtaccagtg ctcgcgctgg
     4861 cctactacca cctgttccgg ccggcgggaa tgacgttcga ggaaggattc ggtctgagcc
     4921 tgccatggtc tcacattggc cgcctcatga cggcggtgct cgccgtcgtc gctgccggcc
     4981 tctggggtga gtggttgatg gatcgcgtgg cggaacagtg gcacctgacc agccattgga
     5041 ccgaatggtt cgacgaggat ttggtgtggg gctcaagctc actcacgagg gtcagtctgc
     5101 ttgagtctgt cattcttgcg ccactcttcg aagagcttgt gttccgaggc ctgttgtttg
     5161 ccatactccg ccgcaaattt cgatttcttc cagcggccct tatgagtgcc tcaattttcg
     5221 gacttgctca tggctacggc gctgttggcc tcatcagcgt ctgttggagt ggtgtcttat
     5281 gggcatggat atatgaaaag acggggagcc tcgcgcccgg aattctggcc cacgccatca
     5341 ataacctctt ggtgtgcctc gcagtgatgt cgcttctccg gttttagccg tcgtccaggg
     5401 gaagggcatt ttgcggacga gcaccacgca cattctattc aactgcgtgt gttctcggtg
     5461 cacgaaagag aagcgtacaa atcgtcgtga cgaatcgatg cgccggcgtt gtatagcatc
     5521 gctagtagct tggcgttgct gatccgcttg ccggcggcgt ggttgt
//
LOCUS       DASSCQ010000208        20509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1124550, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000208 DASSCQ010000000
VERSION     DASSCQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 20509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20509
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124550"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFH05_13190"
     CDS             <1..425
                     /locus_tag="VFH05_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET6679225.1"
                     /translation="RRADNSAVDMRIRIAIGTQLRLKGYQTLPEGRPDFLVTYTIALK
                     DSSPTVSSQYFSEGMAGRPFLHSADSRSVRDAAPSNSEGHSYLTGTLLIDVIDGASEK
                     LVWRGTAAGEVDQGLTSEQRDERTRAIVRSILSHFPPQ"
     gene            complement(479..679)
                     /locus_tag="VFH05_13195"
     CDS             complement(479..679)
                     /locus_tag="VFH05_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6679226.1"
                     /translation="MHIQLSHPARTVEIKGPKRTKDLLRELNLVVEAHLVIRDDELVT
                     EDEMLYDKDRIEIRPVISGGTP"
     gene            complement(744..1748)
                     /gene="galT"
                     /locus_tag="VFH05_13200"
     CDS             complement(744..1748)
                     /gene="galT"
                     /locus_tag="VFH05_13200"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET6679227.1"
                     /translation="MPDLRRDPIVGRWVIISTDRNGRPHDFAQMQPVRPISTGLCPFC
                     PGQERLTPKEIMAYRPEPAEANAPNWSVRVVPNKFPALQVEGDLGREGMGLYDRMNGI
                     GAHEVIIETPVHKDSLADMPTKRLEDVLWAYRDRIIDLKRDVRLRYILIFKNHGAAAG
                     ATLEHSHSQLIALPVVPTSVMNEIEGCRLHYQHKERCMYCDILKQDLAHGERIVAENA
                     EYACVTPFAPRFPFEMWILPKRHAGHFEESEKQHFEFLAPILSESLRRMDRVLSKPAY
                     NFVLHSSPVHEKTGDFYHWHIELIPKLTQVAGFEWGSGFYINPVSPEESARSLREAVL
                     "
     gene            complement(1776..3974)
                     /locus_tag="VFH05_13205"
     CDS             complement(1776..3974)
                     /locus_tag="VFH05_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HET6679228.1"
                     /translation="MRTVRVCFLWHMHQPYYTDPVTRSVSMPWVRLHAAKAYFDMAYL
                     LDKFPRVQATFNFTPSLLVQLQEIGDGSVQDLFWERTQRQAARLNEEEQAFLIKHFFS
                     INWTTMIRPYPRYHELLSKRGFEIEGQDLHRLARQFTAQDFLDLQVWFNLAWFGYGAV
                     ARYPRLAALRAKNRGFTEEEKQEVLALQRSTVQDIVPMYRRLSERGQIELTTTPFYHP
                     ILPLVMDTEHTRRARPDLPLPSRFRAPDDAEAQLRQAVECHLNVFGRPPAGLWPSEGS
                     VCPELIPLLRRVGLRWLATDEGILARSLEASDVPWHRPTQLYRPYRVGPNGEDATIIF
                     RDRDLSDGFGFLYHKTTPDVAAEEVLRRIKQIAIDTPQDHVLLPIILDGENPWEHYQE
                     GGEQFLSHLYQALSDGVLNEGDSIRVATATVSNALEATPDRPYLPHLHSGSWINQDFK
                     IWIGHAEDNRGWDLLNHTRSRLLEISSTLPPDRAKAAWQELYAAEGSDWFWWYGDDFD
                     TTYKEEFDWLFRMHLRNVWTLAGMTPPAQLNQPLCEIRRFEGRDQVTMPLAILHPTLD
                     GVVTDFFEWRGAGTINPNPPLGAMWKAEGLFTAIFFVCDFEHLYLRLDPDEAFKSRTS
                     DLRIECVLQAPRGSYRLMVSSASPDRYRLARRRDDGSWEDIGSWELIGWNYVLELALP
                     FKEIQVGENEQMQMSLLVYEHEMEIARYPRHQPAVVTVPGPEFGATVWRV"
     gene            4054..4485
                     /locus_tag="VFH05_13210"
     CDS             4054..4485
                     /locus_tag="VFH05_13210"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET6679229.1"
                     /translation="MEKTCVESSQRPAAQWLRYWFPVIAYVTLIFYLSSLSHPEERLP
                     NFLFEQLGDKLLHGIEYAVLAVLCYRAFRWAAGPQAGEYAVMLAIVATSLYGLTDELH
                     QAYVPLRESSLADWIADTTGAVIGAVGSRRLMDPGTKPSIS"
     gene            4520..5620
                     /locus_tag="VFH05_13215"
     CDS             4520..5620
                     /locus_tag="VFH05_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6679230.1"
                     /translation="MATIDPVTAKPPLAPPDRALIAETIQSRLPLRATFKKLSPLAGD
                     ASNRRYFRLELGDAAQSVILMQLAEPEAFKQSEEAVSGAGHQIAELPFVNIQAHLAKA
                     GVPVPDLYYYDQTAGLLYLEDFGDLTLSEACAHAGLSQLETRYRQAIDVLVRMQVKAT
                     SPANPQCLAFHRSFDVPLLMWEFDHFLEYGIAAKQGAPIRESDAAAIRQEFRKISELL
                     AAQPRVFVHRDYHSRNLMVDGERLGVIDFQDALMGPATYDLASLLRDAYIALDESLID
                     RLVAYYLDQLQEGRQLWTHRDAFRRLFDFTSIQRNLKAAGRFVYIDRVKGNPKFLADI
                     PRTLGYVKRNLHKYPELFTLRKLMTLYVPELQ"
     gene            5711..6451
                     /locus_tag="VFH05_13220"
     CDS             5711..6451
                     /locus_tag="VFH05_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET6679231.1"
                     /translation="MVLAAGLGTRLRPLTNTIPKPLLPIAGTPLIVWNLLLLKRHGIH
                     DVVINLHHLGPMIEQALGNGSRYGLKIYYSHEPVILGTGGGIKQAECHFSGEPMLVLN
                     GDTLFELDLDALYACHQRRQAAATLVLRSDPDAARWGLVEVDMEDRIVRITGRGKTDV
                     AATKPRMFAGVHILSPRLLRDVPKGKASSIIDAYVAAIERGDAVVGYELTGYWSDVGT
                     PERYAQAEHDATSGLIRLSERLPAAPLA"
     gene            complement(6616..8031)
                     /locus_tag="VFH05_13225"
     CDS             complement(6616..8031)
                     /locus_tag="VFH05_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6679232.1"
                     /translation="MKAKILIVDDDRDIATMLEDRLQASDYGTILAEDGVQALEKVEQ
                     EAPSLMLLDLDMPRLGGLEVLKRLPTIKQAEDIPVIVMTAHGSIEAAVEAMKHGAYDF
                     LTKPLDKDHLLIVIGKALERASLKRHVACLKSEVDSRYASIIGTSTKIRAVLEAAQRA
                     AKSDAGVLLLGESGTGKELLARSIHQWSPRHAMPLVVINCVALTETLLENELFGHERG
                     AFTGADRLQKGKLEMADGGTVFLDEIGDMSLPLQSKLLRVLQDREFHRVGGTRLVSVN
                     IRIIAATNKDLKQAVKDGEFREDLFFRLNVIGLTLPPLRERLEDFPALAKFFLSRHAK
                     EAKRPGMWFTPAALDALSRYSWPGNIRELDNVIARAVILSQTDLIEPEVLALDFNSPV
                     IKEDVIPYLRMPYHESMDAHSRHIIEQALHDADGNQTKAAERLKLQRTYLARLLKQQR
                     QKLEARGDERDEDVTAQTAQL"
     gene            complement(8085..9404)
                     /locus_tag="VFH05_13230"
     CDS             complement(8085..9404)
                     /locus_tag="VFH05_13230"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET6679233.1"
                     /translation="MAQIIPFRGTLYDLASVGSVRNVVAPPYDIIDAAGQKALHDRHP
                     QNIIRLELGMDEPGDNATNNRYTRAAETLRAWIARGALKRDAQPAIYYHTIEYAPPSS
                     DARAPKKTLRGFLTTTKLEPLDSGHIYPHENTRSAAKTDRLNLLKSCKANLSPIWLLY
                     SDPHNAILGLFEKAVKGQPARIDFEDDNGCRQRLWTVTDPSILQQIMDIMASKPLFIA
                     DGHHRYETALNYQRFRRETDGTSGELKPYDSVLMLLSPLEDPGLTVLPTHRVITTPLP
                     PKRQVTARLADTFDVTEFSFTASTTVAVREQFLEALRINGRTAPTFGLALKGDDRYLM
                     LSLKAAHRPSAEASPRTKLDVSLLQQLVVSVLCPTQQEQEAILYTKDDHEALDWAAEG
                     PGTAALLLNATKVSEVQAVATAGERMPHKSTYFYPKPLTGLVIHVME"
     gene            complement(9550..10518)
                     /locus_tag="VFH05_13235"
     CDS             complement(9550..10518)
                     /locus_tag="VFH05_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6679234.1"
                     /translation="MADVWDAYRSELDGVEDRIHQNLTSSVTLVNTVAAHILNSGGKR
                     IRPLLLLLCARLCGYAGREHHQLGSLVEFIHTATLLHDDVVDDADIRRGRQTARKVWG
                     NQVSILVGDYLYSKAMCQVVEFRSQKINEVLAEACKKMAEGEVLQLYYNGNPAMPEGD
                     YIKIVEHKTAGLIAASCRMGAILADASEEQQDCVFRFGQYLGIAFQVVDDTLDYTANG
                     ASLGKTLGQDLRQGKATLPLLHLLNHCSDRDRQMIKDRMETRTLTEGDLQRLIDLMDE
                     SGSIVYALNHAQAYITAAKRELRQFEEGTAKRALSVAADYMVTRDR"
     gene            10775..15292
                     /gene="gltB"
                     /locus_tag="VFH05_13240"
     CDS             10775..15292
                     /gene="gltB"
                     /locus_tag="VFH05_13240"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HET6679235.1"
                     /translation="MELPGLPPKQGLYDPRHEKDSCGIGFVVNIKGHKSHDIIRQGLQ
                     VLENLTHRGAQGCDPCTGDGAGILLQVPHQFLRRAAGDAGVSLPGAGEYGVGQVFLPP
                     HAAARQQCETLFRTIIENEGSRLLGWRDVPVKSDTIGVQARKTEPYMRQVFIARDMLN
                     EAQFERKLYVIRKLVEKAVRESAIQGREHFYIPSLSASTIVYKGLLLPHQMAAYYQDL
                     TDASMVSALALVHSRFSTNTFPTWPLAHPYRHICHNGEINTLKGNVNWMKARQGRLHS
                     DLFGKDMEKVFPIVYEQQSDSACLDNALEFLLLGGRSLPHAMMMLIPEPWVANAQMDL
                     DRRGFYQYHAAMMEPWDGPAAVCFTDGRMIGATLDRNGLRPCRYQVTTDGTVVLASEA
                     GVLPVEAKQIRMKGRLQPGRMFLVDTVQGRIIDDEEIKADIVGRKPYRSWVTQYGVSL
                     DELPEPLNVPQPDHPTIRQRQQAFGYTVEELKMVVTPMIVTGEEAISSMGTDTPLAVL
                     SDRPQLLFKYFKQLFAQVTNPPIDPIREQLVMSLVTNIGPKPNLMDESPESCRRIKVQ
                     QPILTNADLQKIRGISDPHFKSKTLRMLFRVAEGPDGLGAAVDAMCRDASQAIKDGYK
                     FLILSDRGVDEEWAPIPSLLGISAVHHHLVRECTRTEVGLILETGEPRDVHQFACLIG
                     YGAGTINPYLVFESLVDMERDNYLPEGLDAQTAEGKFIKAINKGLLKIFSKMGISTVQ
                     SYCGAQIFEAIGLNHEIIDRYFTGTPSRVEGVGIREIGEETLRRHRVAYEPAAIRQLD
                     FGGEIHYRIQGEHHNWNPDTIYKLQHASKSNDPKTYAEFAQLVNDESTRRSNLRGLLD
                     FKFEQFPLPIEEVEPAREIVKRFNTGAMSFGSISKEAHETLAVAMNRLGGKSNTGEGG
                     EDPERFKPLPNGDSKNSYIKQVASARFGVTAHYLVNARELQIKMAQGAKPGEGGQLPG
                     HKVDENIARFRYATPGVQLISPPPHHDIYSIEDLAQLIFDLKNSNPDAAVSVKLVAEV
                     GVGTVAAGVSKAHADKVLISGDSGGTGASPLSSIKYGGIPWELGLAETHQTLVLNDLR
                     GRIRVETDGQMKTGRDVVIATLLGAEEYGFATAPLIVEGCIMMRKCHLNTCPVGIATQ
                     DPELRKKFNGKPEHIVNYLFFVAEEARKLMAKLGFRTINEMVGRVDKLKITKAVDHWK
                     AKGLDLTPLLTLPDVAPEVPRYCVQKQDHGLDCILDNTLVDLCNPAIEKGEKVQLDLP
                     IKNVNRTTGTVLSSKIAKKYGLEGLPEDTISIKFTGSAGQSFGAFLSRGITLTLEGES
                     NDYIGKGLSGGKIIVYPPKDVLFNPEETILIGNTSLYGATQGEAYFYGMAGERFAVRN
                     SGARAVVEGTGDHGCEYMTGGVVVVLGRTGRNFAAGMSGGIAFVLDDVGRFQSRCNTG
                     MVELEPVSTKEDKQQLHDLITKHFMYTGSRKAKQVLDAFDATLPKFVKIMPVDYKRVL
                     EERKRKAGAVH"
     gene            15362..16795
                     /locus_tag="VFH05_13245"
     CDS             15362..16795
                     /locus_tag="VFH05_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET6679236.1"
                     /translation="MGDPKGFMKYPREGPKRKPVELRVLDWKEMYEPISEDRLKIQGA
                     RCMDCGVPFCQGSTGCPVVNLIPEWNDLVYRGRWQDALKALHTTNNFPEFTGRLCPAP
                     CEGACVLGINEEPVSIRVIEWNIIDRGFNEGLVFPVLPVAKTGKTVAIVGSGPAGLAA
                     AQQLARAGHSVTLFEKADRIGGLLRYGIPDFKMEKWVIDRRLEQLKAEGVEFKTGVTV
                     GTDITGEQLRAQFDAVGLTMGAEQARELPIPGRELKGVHLAMEYLTQQNKRTAGIPIS
                     DEPITAKGKRVVIIGGGDTGSDCLGTTHRQGCVEVHQFELLPEPPPTRAESTPWPLWP
                     MQLRTSHAHEEGCDRQWSVATTKFTGHNGHLTKLHAQRVQFEGGKFTPIPDTEFAMDV
                     DLVLLAMGFTGPIKNGLLDHLGVAYDSRGAVAVDDNFMTNLEGVFAGGDTKRGASLIV
                     WAIAEGRKMAAGIDKYLQAGRSAKSRR"
     gene            17148..17453
                     /locus_tag="VFH05_13250"
     CDS             17148..17453
                     /locus_tag="VFH05_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679237.1"
                     /translation="MHMLMLVFRASLKERVYELLDRCHVKAFTEVNETVGYGQTGPAE
                     GLAFYPGTNSVIFVALDPDHAARVARAVKDWYAQAADHPGWEKPALRVFSWPAEQIV"
     gene            17648..19363
                     /locus_tag="VFH05_13255"
     CDS             17648..19363
                     /locus_tag="VFH05_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HET6679238.1"
                     /translation="METNLNLLLLEDSPADAELMIEALRESGYDPTVRRVDSKDAYLR
                     ELEQPPDFILSDFSMPQFTAQDALKLMKERGLDLPFIIVSGCMGEDMAVECMKAGASD
                     YLLKDRLARLGHAVTQALERKRLVEEKRQAEQRLFLETFHDSLTGLPNRALFMDRLDR
                     VFLQSRRQPAHLFALLHLNLDGLKMVHSGLGVSSADRLLIEVSQRILRRIRSADTLAR
                     MEGDAFTLLIDNLKAMGNATRVADRLQQEFATPFTLEDREVFLTPYIGIASSHTGYEN
                     GEPLLRDATTAMLQAKATGRAGFVIFDKAMHQQAMARLKMEGDLRQALDRKEFRLAYQ
                     PIVQLQEGRITGFEALLRWQHPEHGLTRPDAFLDIATELGLMKGIGEWSLGEGCRQLK
                     LWHDQFPRIRPLTISVNFSIQHFTQDHLALLIKHVLDKSGIDASSVKIEITESEMMQN
                     PEAVNRVLAEIKEQHVDTCLDDFGTGYSSLSHLQQLPITFLKIDQSFVRRLGTDDDAL
                     AIVKTIIALAHQLGRQVIAEGIETAEHLAILRSLGCEYGQGYFFSKPLSPEDAGTLLA
                     SARRW"
     gene            19428..20165
                     /locus_tag="VFH05_13260"
     CDS             19428..20165
                     /locus_tag="VFH05_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6679239.1"
                     /translation="MASVQRFSAKVIAVRQLTHDVRQIDFRLMEPREIRFKAGQFVSF
                     EVPDARTGRTVTRPYSIASAPSTPDRVSLLLNFVPDGPGSTYLFKLNEGDTTTFSGPA
                     GNFYLRDDPERELLFVATGTGIAPLRSMLLANADRPQPSRTVLFWGLRSQRDLYYEEE
                     LARMSRTRPGSSHVITLSRPEPGWHGPSGRVTRLVEREVHDVKRLAVYLCGNGGMITD
                     VTRIIQTRGLCPIFREKYYDDAESGDD"
BASE COUNT         4355 a         5740 c         6088 g         4326 t
ORIGIN      
        1 atcgacgcgc cgacaactcg gcggtggata tgcgcattag aatcgccatc gggacccaat
       61 tgcgattgaa gggctatcag acgcttccag agggcaggcc ggacttttta gtgacgtaca
      121 cgatcgcatt gaaggacagc agtccgacgg tttcgtcaca atatttttcc gaggggatgg
      181 ccgggcgtcc ctttctgcat tccgcggata gccgttctgt ccgtgatgcg gcaccctcga
      241 atagcgaggg gcactcctat ctcacaggca cactgctcat cgacgtcatc gatggcgcat
      301 cggagaagtt ggtgtggcgc ggaacggctg ccggagaggt cgatcagggc ctgacatccg
      361 agcaacggga cgagcgcacg agagcgatcg ttcggagcat tctctctcat ttcccgcccc
      421 agtaattccg cgggatggcg tgaccggcac cttagggaac gtggggcgag ttgcgctgct
      481 atggtgtccc gcctgagatg accggcctga tttcgatccg gtccttgtca tacagcatct
      541 cgtcttcagt caccagttcg tcgtcacgga tgacaagatg cgcctctacc acgagattga
      601 gttcccgcaa aagatccttc gtccgcttcg gccctttgat ttcgacggtt cttgccggat
      661 gactcaactg gatgtgcatg ccggtatctt atgcctgctc catgaacggg agcaaggagg
      721 gaaagtgtgg aggaggctcc agtctagagg accgcttcgc gcagagatct tgcagactct
      781 tccggcgaga ccggattgat gtaaaagcct gagccccatt caaaaccggc gacctgcgtg
      841 agtttcggaa tgagttcgat gtgccaatga taaaagtccc cggttttttc gtgcaccggt
      901 gagctatgga gcacaaaatt gtaggccggc ttggagagca cccgatccat ccgacggagt
      961 gattccgaga gaatcggggc aagaaactca aagtgctgtt tttcgctctc ttcaaagtgg
     1021 ccggcatgcc gcttgggaag aatccacatt tcaaaaggaa acctcggtgc gaacggcgtc
     1081 acgcaggcat actcggcatt ctccgccacg atgcgttcac cgtgcgcaag gtcctgcttc
     1141 aggatgtcgc aatacatgca ccgttctttg tgttgatagt gcagacggca gccttcgatc
     1201 tcgttcatca cgctggtggg aacgacgggg agcgcgatca actgggagtg gctgtgttca
     1261 agcgtcgcgc cggccgccgc gccgtggttt ttgaatatca gaatgtaccg caggcggaca
     1321 tccctcttga gatcgataat ccggtcgcga taggcccaca gcacatcttc tagccgcttg
     1381 gtcggcatgt cggcaagact gtctttatga accggagttt caataatgac ttcatgagcg
     1441 ccaatgccgt tcatccgatc gtagagtccc atgccttcgc ggcctaaatc cccttcgacc
     1501 tgtaaggcag gaaatttgtt gggtaccacc cgaacggacc aattcggagc gttggcttcg
     1561 gcgggttcag gcctataggc catgatctct ttcggagtga gccgttcctg ccctggacaa
     1621 aacgggcata gcccagtcga gatcggccgg acgggctgca tctgcgcaaa gtcatgcgga
     1681 cggccgttcc ggtccgtcga aatgatgacc cagcgcccga caatgggatc gcgccgtaag
     1741 tcaggcatgg cgcaccaaca cgtaaagagg gctgttcata ctctccagac tgtggctccg
     1801 aactccggtc ctggcaccgt cacgaccgcc ggctgatggc gcggatagcg ggcgatttcc
     1861 atttcatgct catacacgag gaggctcatc tgcatctgtt cgttctcccc aacctgtatc
     1921 tctttgaacg ggagagccag ttccaacacg tagttccagc caatcagttc ccaagaaccg
     1981 atgtcctccc agctgccgtc atcacgtcgt cgggccaggc gatagcggtc cggtgaggca
     2041 gaggagacca tgagacgata cgagccgcga ggagcctgca gcacacattc aatccgcaga
     2101 tcgctcgttc gggatttgaa cgcctcgtcc ggatcgagcc gcagatagag gtgctcgaaa
     2161 tcacagacga agaagatggc ggtaaacagg ccttccgctt tccacattgc gcccagcggt
     2221 ggattcgggt taatggttcc ggcgccccgc cattcgaaga aatccgtcac cactccatcc
     2281 agtgtgggat gtaggatggc cagaggcatc gtaacctgat cgcggccctc gaagcgccgg
     2341 atctcgcaca gcggctgatt gagctgagcc ggcggcgtca tgccggccag cgtccagacg
     2401 ttgcgaagat gcatacgaaa aagccaatcg aactcttctt tataggtcgt atcgaaatcg
     2461 tcaccgtacc accaaaacca gtcactgcct tcggcggcgt atagctcttg ccaggccgct
     2521 ttggcacgat ccggcggcaa tgtggaactg atttcgagca ggcgtgagcg ggtgtggttc
     2581 agcaggtccc atccccggtt gtcttctgcg tggccgatcc agatcttaaa atcctggttg
     2641 atccatgagc ccgaatgcag gtgagggaga tacggccgat ccggtgtggc ctccaacgcg
     2701 ttggagacgg tggcagtggc cacacgaatc gagtcgcctt cgttgagcac accatcggaa
     2761 agggcctgat agagatgtga gaggaactgc tcgccgcctt cctgataatg ctcccatgga
     2821 ttttcgccat ctaagatgat ggggagcaga acgtggtcct gcggcgtatc gatggcgatc
     2881 tgctttatcc gtcggaggac ctcctctgca gccacgtcgg gtgtcgtctt atggtagaga
     2941 aagccaaacc cgtctgacag gtctcggtcc cggaagatga tcgtcgcgtc ctctccattg
     3001 ggtcctacgc gataaggccg atacagctgg gtcggccgat gccaggggac gtccgaggct
     3061 tcgagcgatc gggccaggat gccttcgtcg gtagcgagcc agcggagacc gacacggcga
     3121 aggagcggaa tcaattcggg gcagacagac ccttcggaag gccaaagacc agccggcggc
     3181 ctgccgaaca cgttcagatg gcattcgaca gcttggcgca gctgggcctc ggcatcatcc
     3241 ggtgctcgga atcgtgaagg caacggaaga tccggtctgg cccggcgcgt gtgctcagtg
     3301 tccatgacca gtgggagaat cggatgatag aacggcgtcg tcgttaactc gatctgcccg
     3361 cgctcggaca ggcgccggta catgggcaca atgtcctgga ccgtgctgcg ctgcaacgcc
     3421 aacacctcct gcttttcttc ttcggtgaag ccccgatttt tggcgcgtaa ggcggcgagt
     3481 cgtggatacc gggcgacggc gccatagccg aaccaggcca gattgaacca tacttgtaaa
     3541 tccaggaagt cctgcgccgt gaactgccgg gcgagccgat ggagatcctg cccctcaatc
     3601 tcgaacccgc gcttgctcaa tagttcgtga tagcgggggt acggtcggat catggttgtc
     3661 caattgatcg agaagaagtg cttaatgagg aatgcctgct cttcttcgtt caagcgggca
     3721 gcctgccgct gcgtacgttc ccagaacaga tcctgaactg agccgtcccc gatctcttgt
     3781 agttgaacca gtaacgacgg cgtaaaattg aacgtggcct gcacccgcgg gaacttatca
     3841 agcagatacg ccatgtcaaa gtacgccttt gccgcgtgga ggcggaccca gggcatgctg
     3901 accgaccggg tcaccgggtc cgtatagtag ggctggtgca tatgccagag aaaacagact
     3961 cggacggttc tcataggcaa tgtgtgtaaa ggatattgtg gagtagcaca gagtatgtca
     4021 tagggatact gaggtggcaa tcaacggagg aaaatggaaa agacctgtgt ggaaagcagt
     4081 cagcgcccgg cagcgcaatg gctgcgatac tggtttccgg tgatcgcgta cgtcacgttg
     4141 attttttacc tctcatcgtt atcccatcct gaggagcggc tgcccaactt cctctttgaa
     4201 caactgggtg acaagctgtt gcatgggatc gagtatgcgg tgctggcggt gctctgctat
     4261 cgagcctttc gttgggccgc aggaccgcaa gccggagagt atgccgtcat gctcgcgatc
     4321 gtcgcgacgt ccttgtatgg cctcacggat gaacttcatc aagcctacgt gcccctccgc
     4381 gagtccagtc tcgccgattg gatcgccgat acgacggggg cggtgatcgg cgcagtcggg
     4441 tcacgccgcc ttatggaccc cggcaccaag ccgagcattt cgtgacaagc ttttcacgca
     4501 acagtataat gccgcgctga tggccacgat cgatcctgtc accgccaaac cgcccctcgc
     4561 tccgccggac cgggcgctca ttgcggaaac gatacagagc cggttgcctc tccgagcgac
     4621 gttcaagaaa ctgtctcctc ttgccggtga tgcctcgaac cgtcgctatt ttcggctcga
     4681 gctcggcgat gccgctcagt cggtcatcct gatgcagctg gccgagccgg aggccttcaa
     4741 gcagtcggaa gaggccgtga gcggggcagg tcatcagatt gccgagctgc ccttcgtgaa
     4801 catccaggcg catcttgcca aggccggggt accggtcccc gacctctact attatgatca
     4861 gacagccggg ttgctgtatc tcgaggattt tggagacctc acgctgtcag aggcctgtgc
     4921 ccatgccggt ctatcgcaac tcgaaacccg ctatcggcag gccatcgatg tgctcgtgcg
     4981 tatgcaagtg aaagcgacgt ctcctgccaa tcctcaatgc ctcgcattcc atcggagctt
     5041 cgacgtgccg ctgttgatgt gggaattcga tcactttctc gaatacggga tcgcggcgaa
     5101 gcagggagcg ccgatacgtg agagtgatgc agccgccatc cgccaagagt tccgcaagat
     5161 ttccgaattg ctcgcggcgc aaccacgagt cttcgtacac cgcgattacc attccagaaa
     5221 tttgatggtc gacggagagc gattaggcgt gattgatttt caggacgccc tgatgggccc
     5281 tgcgacctac gatctggcct ctttgctccg cgacgcatac atcgcattgg acgagtcact
     5341 cattgaccgg cttgtggcct attacctcga tcaacttcag gaaggccgtc agctgtggac
     5401 tcatcgagac gcgttccgcc gtctgttcga cttcacgtcc attcagcgca atctcaaggc
     5461 ggccggccgg tttgtgtata tcgatcgggt gaaaggcaac ccgaaattcc tggccgatat
     5521 tcctcgcacg ctcggatatg tcaaacgcaa tttgcacaaa tatcccgaat tgttcacgct
     5581 gcgcaaactg atgactctgt atgtgccgga actgcagtaa ggggactgac taccggaatc
     5641 tgaaatctgg cggccatccg gctcgctcag tgttcggcac gaccggctca gcgttggccc
     5701 agtgaaagcc atggtccttg ccgccggctt gggcacacgg ctgcggccgc tcaccaatac
     5761 gattcccaaa cccctgctcc ccatcgcggg aacacccttg atcgtgtgga atttgctgct
     5821 tctgaaacgt catggcattc atgacgtcgt catcaacttg caccatttgg gtccgatgat
     5881 cgagcaggcg ctcggaaacg gatccaggta cgggctcaag atctattact cgcatgaacc
     5941 ggtgattctc gggaccggcg gcggcatcaa acaggcggaa tgccatttct caggcgagcc
     6001 tatgctggtc ctgaacggcg atacgctgtt tgagctggac ctcgatgcgc tctatgcctg
     6061 tcatcagcgg cgtcaggcgg cagccacctt ggttctgcgg tcggatcccg atgcagcgcg
     6121 ctggggattg gtcgaagtag acatggaaga ccgtatcgtt cggatcacgg gtcgcggcaa
     6181 gacggacgtc gctgcgacga aaccgcgcat gttcgccggc gttcacatac tcagcccgcg
     6241 tctgttgcgg gacgtgccca agggcaaagc gtcttcgatc atcgatgcgt acgtcgcggc
     6301 gatcgagcgg ggagacgcgg tggtcgggta tgagctgacg ggctattggt cggatgtcgg
     6361 gacgcccgag cgttatgcac aggcagaaca cgatgccacc agcggtctga ttcggctcag
     6421 tgaacggttg cccgccgctc ccttggctta gcaggtggtg aagagatttt ggcggaggcg
     6481 ctcaccaaat cgcgtccgtc cgcaaccatg acatcggact tcgcagtttg gtgtggagca
     6541 acgcggtttc ctgtcctggg cgcgcagggc ttgaggacgc ggcctggtga ccatccttga
     6601 gaccgggttc cttcttcaca gttgtgcggt ctgtgccgtg acatcctcgt ctctctcgtc
     6661 gcctctcgct tcgagtttct gccgctgctg tttgagcagt cgcgccaagt aggtgcgttg
     6721 gagcttgagg cgctcggcgg ctttcgtctg gttcccatca gcatcgtgta atgcctgctc
     6781 aatgatatgc cggctgtgtg cgtccatcga ttcgtggtag ggcatcctga gatacgggat
     6841 gacatcctct ttgatgaccg gtgaattgaa atcgagcgcc aggacttcgg gttcgatgag
     6901 gtcggtctgg ctcagaatga cagcccgggc gatgacattg tccaattctc ggatgttgcc
     6961 cggccacgaa tagcgggaca aggcgtccag ggcagcaggg gtgaaccaca tgccgggacg
     7021 cttggcttct ttggcatggc ggctcaagaa aaattttgcc aatgcaggaa agtcctccag
     7081 ccgctcgcgc agcggcggca gagtcaggcc gatcacattc agacggaaaa acagatcttc
     7141 gcggaattcc ccatccttca cggcctgctt cagatccttg ttggtggcgg caatgattcg
     7201 gatattcacc gacacgagtc tcgttccgcc gacacgatga aattcccgat cctgaaggac
     7261 ccgcaagagc ttggactgca gtgggagcga catgtctccg atttcatcga gaaacacggt
     7321 gccgccgtcg gccatttcaa gcttgccttt ttgcagacgg tcggcgccgg tgaaggcgcc
     7381 gcgttcatga ccgaataatt cgttttccaa taacgtttcg gtcaaggcga cacaattgat
     7441 gacgaccagc ggcatcgcgt ggcgcggact ccactgatga atggaccgcg cgagcagttc
     7501 cttgcccgtg ccgctctccc cgagcaggag cacgccggcg tccgatttcg cggcacgctg
     7561 agcagcttcc aggacggccc tgatcttcgt actggtgccg atgatcgatg cgtagcgact
     7621 gtccacctct gacttgagac aggcgacgtg ccgtttcaac gacgcacgct cgagcgcctt
     7681 accgatcaca atcagcagat gatccttgtc gaggggcttc gtgaggaaat catatgcgcc
     7741 gtgcttcatt gcctcgactg ccgcttcaat tgaaccatgg gcggtcatga cgatgaccgg
     7801 aatgtcctcg gcttgtttga tcgtcggcag gcgtttcagt acctccagac cgccgagtcg
     7861 gggcatgtcg aggtccagga gcatgaggct aggagcctcc tgctccactt tctccagggc
     7921 ctggacgcca tcttccgcga ggatcgtgcc atagtcggag gcctgaaggc ggtcttccaa
     7981 catcgtcgca atgtcccgat cgtcgtcgac gatcagaatt ttagctttca cgtggttgcc
     8041 ctccatcgcc tagtcccccc aattgatgtc caactctccg gtcatcattc catgacgtgg
     8101 atgaccagcc ctgtcagcgg cttcggataa aaatacgttg atttgtgcgg cattcgttcg
     8161 cctgctgtcg ccaccgcctg gacttcgctc actttggtcg cattgagcag cagggcggcc
     8221 gtccctggtc cttcagctgc ccaatcgagc gcttcatgat cgtccttggt gtagaggatg
     8281 gcttcctgct cttgttgtgt cgggcacagc acgctcacga ccaattgttg aagcagtgag
     8341 acgtccagct tcgtacgcgg ggaagcctca gcggaaggac ggtgcgccgc cttgagcgag
     8401 agcatcagat agcgatcatc tcccttgagt gcgagaccga aggtcggagc ggtgcgtccg
     8461 ttgatgcgca atgcttccag gaattgttct cgcactgcaa ccgttgtcga tgccgtaaac
     8521 gaaaactcgg tcacgtcgaa tgtgtcagcc aaacgggcgg tgacctgccg cttaggcggg
     8581 agcggcgtcg tgatcacacg gtgagtggga agtacggtca agcccgggtc ttcgagggga
     8641 gacaacagca tgaggacgct gtcatagggc ttcaactcgc cggaagtccc gtctgtttcc
     8701 cgccggaagc gttgataatt gagcgccgtc tcgtaccgat gatggccgtc ggcaatgaac
     8761 agcggtttgc tggccatgat gtccatgatt tgctgaagga tggaaggatc ggtcaccgtc
     8821 cacagccgct gccggcagcc attgtcgtcc tcaaaatcga tccgagccgg ttggcccttc
     8881 acggcctttt caaacaggcc caagatcgca ttgtggggat ccgaatagag cagccagatc
     8941 ggactgagat tcgccttgca tgacttgagc agattcagcc tgtcggtttt tgcagccgac
     9001 ctcgtgtttt catgagggta gatgtgacca gagtccagcg gctccagttt cgtcgtcgtc
     9061 agaaagcccc gcagcgtctt cttcggcgcc cgggcatccg acgatggagg cgcgtactcg
     9121 atcgtgtgat agtagatggc cggttgcgcg tcccgcttaa gcgcgccacg cgcgatccag
     9181 gctcttaaag tctcggccgc acgcgtatac cggttgttcg tggcattgtc gccaggctca
     9241 tccatgccga gctcaaggcg aatgatattt tgcggatgcc ggtcgtgcaa cgctttttgc
     9301 cctgcggcat cgatgatgtc atagggcgga gccacgacgt tgcggacgga cccgaccgac
     9361 gccaggtcgt acaacgttcc ccgaaatgga atgatctgtg ccatacgttc ctctctgcag
     9421 gatgtgaaaa tcgttgctgc ggccgcgccg gacggtcttt gaccatcctg cgggttcctt
     9481 caaaggctgg gccgccattt gcgcgtgttg cccgtctcgt gccgaatggc gtgagaccac
     9541 gcggagcggc taccggtcgc gggtcaccat gtaatctgca gctaccgaga gagcccgttt
     9601 cgcggtgccc tcttcgaact gtctcaactc tcgcttcgcg gcggtgatgt aggcttgagc
     9661 atgattcaag gcgtagacaa tggatccaga ctcatccatc aggtcaatca gccgctgtaa
     9721 atcgccttcc gtcaacgtgc gtgtttccat ccggtctttg atcatctgcc gatctctgtc
     9781 ggagcaatgg ttcagcaaat gaaggagggg gagcgtggcc ttaccctggc ggagatcctg
     9841 gccgagcgtc ttccccaaag aggccccgtt cgccgtatag tccaatgtgt catcgacgac
     9901 ttgaaacgcg atgccgagat attgtccgaa gcggaacacg cagtcttgct gttcttcgga
     9961 cgcatcggcc aaaatcgcac ccatccggca ggaggcggcg atcagtcccg ctgtcttatg
    10021 ctccacgatc ttaatgtagt cgccttccgg catcgcaggg ttgccattgt agtacagctg
    10081 gagtacttca ccttcagcca ttttcttgca ggcctcggcg agtacttcgt tgatcttttg
    10141 gctgcggaac tcgacgacct ggcacatggc cttggaatag aggtagtcgc ccaccagaat
    10201 actgacctga tttccccaaa cttttcgagc ggtttgacgg ccccgcctga tgtcggcatc
    10261 atcgacgaca tcgtcatgca gcagggtcgc cgtgtggatg aactccacga ggctgccgag
    10321 ttgatggtgc tcgcggcctg cgtagccgca gaggcgcgcg cagagcaaga gcaggagggg
    10381 ccggatgcgc ttgccaccgc tgttgagaat atgcgcggcg accgtattga cgagggtgac
    10441 gctcgaagtc agattctgat ggatgcgatc ttcgacaccg tccagctcac tacgataggc
    10501 atcccatacg tccgccatcg tgtgaatcga ggaggtgaca ggaccctgtg gcatgcggcg
    10561 gatggtaggg cagcggatga aacaaagtca agggaatcgc ggaggaatcc atgcattgtc
    10621 ggcttgacag aagaatcaac cttccattaa tctgacccac gaatttgaac gaagacatgc
    10681 ctcgcgagca aggggtttcc gcatccctga ttcctctcga tagggcctct actcaacagg
    10741 aaaaagacct gcctttttca ctggataggt aagaatggaa cttccagggc ttccaccgaa
    10801 gcaagggctc tacgaccctc gacacgaaaa agattcgtgc gggattggat ttgtcgtcaa
    10861 catcaaaggt cacaaatcgc acgacatcat ccgccagggc ctgcaagtgc tcgaaaatct
    10921 cacccaccgt ggcgcgcagg gatgtgatcc gtgtactggt gacggcgcgg gaatactttt
    10981 gcaagtcccg catcaattcc tgcggcgtgc ggcgggtgat gccggcgtga gtctgccggg
    11041 agccggtgaa tacggcgtag gccaggtgtt tctgccgccc catgccgctg cacggcagca
    11101 atgtgaaacc ttgttccgca cgatcatcga gaacgaaggc tcacggctgc tcggatggcg
    11161 agacgtgccg gtcaaaagcg acacgatcgg cgtgcaggcg cgtaaaactg agccctacat
    11221 gcgccaagtg ttcattgctc gcgacatgct gaacgaggcg caatttgaac gaaaactcta
    11281 cgtgatccgt aagctggtcg aaaaagccgt gcgtgaatcg gccattcagg gccgcgaaca
    11341 tttctacatc cccagtctct ctgccagcac gatcgtctac aaaggcttgc tgctgccgca
    11401 tcaaatggcg gcgtattatc aggatctcac agacgccagc atggtcagcg cgctggcgct
    11461 cgtacattcg cgcttcagca ccaacacgtt tccgacctgg ccgctcgccc atccctatcg
    11521 tcacatctgc cataacggtg aaatcaacac cctcaagggc aatgtgaatt ggatgaaggc
    11581 ccggcaggga cggcttcatt ctgatctgtt cggcaaggac atggaaaaag tgttccccat
    11641 cgtgtatgag cagcaaagcg actcggcgtg tctcgataat gcgctcgagt ttttgctgct
    11701 gggaggccga tcgttgcccc acgcgatgat gatgttgatt cccgagccgt gggtcgccaa
    11761 tgcccagatg gatctggatc gtcgcgggtt ttatcaatat cacgccgcca tgatggagcc
    11821 ctgggacggt ccggccgcgg tctgtttcac ggatggcagg atgatcggag cgacgctgga
    11881 tcgcaacggg ctccgcccct gccgctatca ggtcaccacg gacggcacgg ttgtgctggc
    11941 gtcggaagca ggtgtcttgc cggtcgaggc gaagcagatc cgcatgaagg gccggttaca
    12001 gcccgggcgg atgttcctgg tcgacaccgt gcagggccgc atcatcgatg acgaggagat
    12061 caaggccgac atcgtcggcc gcaagccgta tcgcagttgg gtcacacagt acggcgtctc
    12121 actggacgaa ctaccggaac cgttgaacgt cccgcagccg gatcatccga ccatccgtca
    12181 gcgacaacag gcattcgggt acacggtgga agagctcaaa atggtcgtca cgccgatgat
    12241 cgtgacgggc gaagaagcca tctcatcgat ggggactgat acgcccttgg ccgtgttgtc
    12301 ggaccgcccg cagctgctgt tcaaatactt caagcagctg ttcgcccagg tcacgaaccc
    12361 gccgatcgat ccgatccgcg agcagctcgt gatgtcgctc gtcacgaaca ttggtccgaa
    12421 gcccaacttg atggacgagt cgccggagtc ctgccgccgg atcaaggtgc agcagccgat
    12481 tctcaccaat gccgacctcc aaaaaatccg cggcatctcc gatccgcatt tcaagagcaa
    12541 aacgctgcgc atgctcttcc gtgtggccga aggaccggac ggattgggcg ccgcggtgga
    12601 cgccatgtgc agagacgcct ctcaagcgat caaggacggc tacaagttcc tgatcttgag
    12661 tgaccggggg gtcgacgaag agtgggcgcc gattccgagt ctgctgggta tctccgcagt
    12721 tcatcatcat ctcgtgcgcg agtgtaccag aaccgaggtg ggactgattc tcgaaacggg
    12781 cgagccccgg gacgtccatc aattcgcctg cctgatcggc tacggcgccg gaaccatcaa
    12841 tccctatctt gtattcgaat cgctcgtcga catggagcgc gacaactatc tgccggaagg
    12901 gcttgatgcg cagacggccg aaggcaaatt catcaaggca atcaacaagg gcctcctcaa
    12961 aatcttctcg aagatgggca tctcaaccgt ccagtcgtat tgcggtgcgc aaattttcga
    13021 ggcaatcgga ttaaaccacg aaattatcga ccgttatttc accggcacgc cgtcacgcgt
    13081 ggaaggcgtg ggaatccggg agatcggcga agaaaccctg cgacggcatc gcgtcgcgta
    13141 cgagcctgcc gccatccggc agctcgattt cggcggcgag atacattatc ggatccaggg
    13201 agagcaccat aactggaatc ctgacaccat ttacaagctg cagcacgcca gtaagtcgaa
    13261 tgatcccaag acgtatgccg agtttgcgca gcttgtgaac gacgagagca cgcgccggtc
    13321 caacctgcgc ggcctcctcg attttaaatt cgagcagttt ccgctgccca tcgaagaagt
    13381 ggagcccgca cgtgaaattg tgaaacggtt taacacgggt gcgatgtcgt tcgggtcgat
    13441 cagcaaagaa gcgcatgaaa cattggcggt tgcgatgaac cgtctgggcg gcaagagcaa
    13501 taccggcgaa ggcggcgaag atcctgaacg gtttaagccc ctgccgaacg gcgattccaa
    13561 gaacagctat atcaagcagg tcgcctcggc tcgcttcggg gtcactgctc attacttggt
    13621 gaatgctcgc gaattgcaga tcaaaatggc gcaaggcgcc aagccgggtg agggcggcca
    13681 gttgccgggc cacaaagtgg acgaaaacat cgcgaggttc cgttatgcga cgcccggggt
    13741 acagctcatt tcgccgccgc cgcaccatga tatctattca atcgaagacc tggcgcaact
    13801 catttttgat cttaagaaca gcaatccgga tgcagccgtg tccgtaaaac tggttgcgga
    13861 agtgggtgtc ggcaccgtcg ccgcaggcgt atcaaaagct catgcggata aggtcctgat
    13921 cagcggtgat tccggcggaa ccggtgcctc gccgctttct tcgatcaagt atggcggaat
    13981 tccatgggag ctgggtcttg cagaaacaca ccagacactt gtgctcaacg atttgcgcgg
    14041 gcgcatcagg gtcgaaacgg acggccagat gaaaactgga cgagatgtcg tcatcgcgac
    14101 cttgctcgga gccgaagagt acggctttgc gacagcgcca ctgatcgtcg aaggttgcat
    14161 catgatgcgg aagtgccatt tgaacacctg ccctgtcggg atcgccaccc aggatccgga
    14221 gctgcgcaaa aagttcaacg ggaagcccga acacattgtg aactatttat tcttcgtcgc
    14281 cgaagaagcg cggaaattaa tggccaagtt ggggtttcgc acgatcaacg agatggtcgg
    14341 tcgcgtggac aaactcaaga tcacgaaggc cgtcgatcac tggaaggcga agggactgga
    14401 tctgacgcca ttattgacct tgccggatgt ggcaccggaa gtgccgcgtt attgcgtcca
    14461 gaaacaggac catgggctgg actgtattct ggacaacacg ctcgttgacc tatgcaatcc
    14521 ggccattgag aagggggaga aggtccaact cgatctgccg atcaaaaacg tgaaccgcac
    14581 cactggaacg gtgctttcca gcaagatcgc aaagaaatat gggttggaag gactgcctga
    14641 ggacaccatc tcgattaagt tcacagggtc ggccgggcaa tcgttcgggg cctttctttc
    14701 caggggtatc acgctcacgt tggaagggga atccaacgac tatatcggca agggcctgtc
    14761 cggcggcaag atcattgtct atccgccaaa ggacgtgttg ttcaacccgg aagagacgat
    14821 cctcatcggg aatacatcgc tgtatggagc gacgcaaggt gaggcctact tctacggaat
    14881 ggcgggggag cggtttgctg tgcgaaacag cggggcacgg gcggtcgtcg aaggcacggg
    14941 tgatcacggt tgcgaataca tgaccggtgg ggtggtggtg gtgctcggtc gaaccggtcg
    15001 caattttgcg gcaggcatgt ccgggggcat tgcattcgtg ctcgacgatg tcgggagatt
    15061 ccagagccgc tgcaacacag gcatggttga actcgagccg gtctcgacaa aggaagacaa
    15121 gcagcagctg cacgatctga ttacaaagca tttcatgtat acgggcagcc gaaaggcaaa
    15181 acaggtgctc gatgcattcg atgccacgct gccgaaattc gtcaagatca tgccggtcga
    15241 ctataaacgc gttctcgaag aacgtaaacg aaaagccggc gcggtgcatt gaaatgtgaa
    15301 gcatcgttcg tgacgcgtga aggcgcggtc gagcggcaag atacgaaaga ccaacgacga
    15361 aatgggtgac ccaaaaggtt tcatgaaata cccccgcgag gggcccaaac ggaaaccggt
    15421 cgagctccgg gtgctcgatt ggaaggagat gtacgagccc atttccgagg acagactcaa
    15481 aattcaaggg gcgcgctgca tggattgcgg cgtgccgttt tgccagggaa gcaccggctg
    15541 ccccgtcgtg aacctcatcc cggaatggaa cgatctcgtc tatcgggggc ggtggcagga
    15601 tgccctcaag gctttgcaca cgaccaataa ctttccggaa ttcaccggcc gactctgtcc
    15661 ggctccgtgc gaaggcgcct gcgtgctcgg catcaatgaa gaaccggtgt ccattcgcgt
    15721 gatcgaatgg aacatcatcg atcgagggtt caatgaaggc ctcgtatttc ccgttctgcc
    15781 ggtggccaaa acgggaaaaa ctgtggcgat cgtcggatcc gggccggccg ggcttgcggc
    15841 ggcgcagcaa cttgcgcgcg caggccatag tgtcacgctc tttgaaaaag cagaccgcat
    15901 cggcggcttg cttcgctacg gcattcccga tttcaaaatg gaaaagtggg tgatcgaccg
    15961 ccgcctggag cagctgaagg cggaaggcgt ggaattcaag acgggcgtca ccgtcggcac
    16021 agacattacg ggcgaacaat tgcgcgcgca gttcgatgcg gtgggcctca ccatgggagc
    16081 cgagcaggcg cgtgaactgc cgattcctgg aagggaactc aaaggcgtgc atttggcgat
    16141 ggagtatctg acgcagcaaa acaagcgcac ggcaggcatt cccatcagcg acgaaccgat
    16201 caccgccaag ggcaaacggg tcgtcattat tggcggggga gacaccggat ctgactgtct
    16261 gggaaccacg caccgtcaag gctgtgtgga agtgcatcag ttcgagttac tgccggagcc
    16321 gcctccgaca cgtgccgagt cgacgccgtg gccgctgtgg ccgatgcagt tgcggacttc
    16381 tcatgcccat gaggaagggt gcgaccgtca gtggagcgtc gcaacgacca agttcaccgg
    16441 ccacaatggc catctcacga agctacacgc gcaacgcgtg cagttcgaag gcggcaagtt
    16501 tacgccgatt ccggacaccg aatttgcgat ggacgtggat ctggtcctgc tcgcgatggg
    16561 gttcactgga cctatcaaaa acggccttct cgaccatctc ggggtggcct acgactcccg
    16621 cggtgcggtt gccgttgacg acaacttcat gacgaacctc gagggggtct ttgccggcgg
    16681 cgacacgaag cgcggtgctt ccctcatcgt ctgggcgatc gccgaaggcc gaaagatggc
    16741 agcgggaatc gataagtacc tgcaagccgg tagatccgcg aagtcacgtc gttgagcgca
    16801 cggccaaggc tgtctccgaa tctcctcctt ccccctctca cgtgccgtca gtcacaaacg
    16861 tacagaggct gaggcgaacc aggcaatgac gcatgtcgcg aggctgcgca tgtggaaccg
    16921 acttgggaga ctgtgtgaaa cgtgcaggag ctccgaggtg tttcgtgcgg ccttgtgtat
    16981 cgcggacgat ctacgctatc catgtgtgga ccaggtgatg gcggccttgc gcccaggaag
    17041 tgtgtccacc agtttgctgc ctcctgtgtc cagatgggca catctcattg aactggagga
    17101 cgaccggtca tgagtcgtgc caccaatgac gaaggaggaa acttcgcatg catatgctca
    17161 tgctggtgtt ccgtgcgtca ctgaaagaac gtgtgtatga attgctcgat cgttgtcacg
    17221 tgaaagcgtt tactgaggtc aatgagacgg ttgggtatgg tcagaccggc cccgccgaag
    17281 ggttggcctt ttaccccggc acgaattctg tcatcttcgt tgccttggat cccgatcatg
    17341 cggcgcgagt cgccagggcc gtcaaggact ggtatgcgca ggcggcagat catcccggct
    17401 gggagaaacc ggccctgcgc gtcttctcct ggccggcaga gcagatcgta tgacgggtcc
    17461 gagagttcgg aatcctgtcc attattccgc tgcgcagtcc tcatagggtt catgcttagg
    17521 ctctgataag ttccaaacat ttcttggtcg tttgctcatt ctcactctat actgcctacc
    17581 tacgtagctg tgcatagaag gaaggacggc ttcccgtgaa gcggtccgac ctcgatcgag
    17641 gaccaacatg gaaaccaacc tcaatctcct gctgctggaa gattcaccag ccgatgcgga
    17701 gttgatgatt gaagcactgc gagagtcggg gtatgacccc accgttcgcc gcgtggattc
    17761 caaggacgcc tatctccgag aactcgaaca gccgccggat tttatcctgt ccgatttttc
    17821 catgcctcag ttcacggcgc aggacgctct caagttgatg aaggaacggg ggttggatct
    17881 tccattcatc atcgtctcag gctgcatggg cgaggatatg gcggtggagt gtatgaaagc
    17941 cggtgcttcc gactacctcc tgaaagaccg tcttgcaagg ctcgggcatg cggtgacgca
    18001 ggcgctggaa cgaaagcggc tggtcgaaga aaaacgacaa gctgaacagc ggttgttttt
    18061 agaaacgttc cacgattcgc tgaccggctt accgaatcgg gccttattca tggatcgttt
    18121 ggatcgtgtg tttctacaga gtcgccgtca accggcccat ctcttcgctc ttctgcacct
    18181 gaacctggat ggcttgaaaa tggtgcacag tgggttggga gtttcgtctg ccgaccggct
    18241 cctgatcgaa gtcagccagc gaattctgcg cagaatccgg tccgcagata cgctcgctcg
    18301 gatggaggga gatgcgttta cgctcctgat cgataatctc aaggcgatgg gcaatgcgac
    18361 gcgtgtggca gaccgtctgc aacaggagtt cgccaccccg ttcacgttgg aggaccggga
    18421 agtgttcttg acgccgtaca tcgggatcgc cagcagtcac acagggtatg aaaatggaga
    18481 gccgctgctg cgtgatgcga cgacggccat gttacaggcc aaggcgaccg gccgtgcggg
    18541 attcgtgatt ttcgataaag cgatgcacca gcaggccatg gccagactga aaatggaagg
    18601 ggatctgaga caggcgctgg accgtaagga gtttcgattg gcctaccagc cgatcgtcca
    18661 gttgcaagaa gggcggatca cagggttcga agcgttgctc cgatggcaac atccagagca
    18721 tggattaacc aggccggatg cctttctgga tatcgcgacc gagctgggct tgatgaaggg
    18781 tattggtgaa tggagtcttg gtgaaggatg tcgccaattg aaactctggc acgatcaatt
    18841 tccccggatt cgcccgctca ccatcagtgt caatttctct attcaacatt ttactcagga
    18901 ccacttggcg ctgctgatca aacatgtcct cgacaagtct ggcatcgatg cctcgagcgt
    18961 caagattgaa atcaccgaat cggaaatgat gcagaacccg gaggccgtca atcgcgtact
    19021 cgcagagatc aaggaacagc acgtcgacac ctgcttggac gatttcggaa ccgggtattc
    19081 gtcactgagc catctccagc agttgcccat cacctttctc aagatcgacc agtcgttcgt
    19141 ccgtcgattg ggtaccgacg acgacgcgtt ggcgatcgtc aagacgatca ttgcactggc
    19201 ccaccaactc gggcggcagg tgatcgccga gggcattgaa accgccgagc accttgccat
    19261 cttgcgttcg ctgggctgcg aatacggtca aggctatttc ttttcaaaac cactgtcccc
    19321 cgaagatgca ggcaccctgc tggcctctgc cagacggtgg tgaggcctcc tgtgtccttt
    19381 tcggcaagct cccttgataa gatgagacgt gcgaagggag tggcgtcatg gcttccgtgc
    19441 agcgtttttc ggcgaaggtg atagccgtcc ggcaactcac gcatgacgtc cgtcagattg
    19501 atttccggtt gatggagccg cgtgagatcc ggttcaaagc gggccagttt gtttcgttcg
    19561 aggtgcccga tgcccgaaca gggcggacgg tgacccgacc gtattccatc gcctctgctc
    19621 caagtactcc cgatagggta tccttgctgc tcaatttcgt tccggacggg ccagggtcca
    19681 cgtatctttt taagttgaac gaaggtgaca caaccacgtt ttccggaccg gccggcaatt
    19741 tttacctgcg ggacgatccc gaacgagagc tgttgttcgt ggccaccggg acgggaatcg
    19801 cgcccctacg ctccatgcta ttggccaatg ccgaccggcc tcaacccagt cgcacagtgt
    19861 tgttttgggg tcttcgaagc cagcgcgatc tgtactatga ggaagaactg gcccgcatga
    19921 gccgtacccg accgggaagc tcgcatgtca tcacgctctc acggcctgag cccggatggc
    19981 acgggccgtc aggcagggtg acaaggctgg tcgaacgaga agttcacgac gtcaagcggt
    20041 tagccgtcta tctttgcggt aatggaggca tgattacgga tgtcacccgc atcattcaaa
    20101 caaggggact ttgtccgatc ttccgtgaga agtactatga tgacgctgaa agcggcgacg
    20161 actaataagt gcatttgatg acagggcatt gaggcaaaat ggtatacgag tgtagctctt
    20221 cggattcaga agtaatacgc agtcagacat ccgtcgattc caaaagaagt gaactgcagt
    20281 tggaatcaga gagtggtcgt cttggtggaa ttggcaccag gtttgctcta ggttgtgaca
    20341 accattgaaa ggggataggc ccctgacact gggaaaatta caggaggaca ggatgaagaa
    20401 gacgatttcc gcggtgaccg ctgctgcatt ctttgccgta tgtgccacgg ccttcgccca
    20461 aggccccgct ccagaatcag ctcctgcttc acccccgaat caagacgtg
//
LOCUS       DASSCQ010000209         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_799552, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000209 DASSCQ010000000
VERSION     DASSCQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799552"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFH05_13265"
     CDS             complement(<1..403)
                     /locus_tag="VFH05_13265"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET6679240.1"
                     /translation="MKRSRPFNLLVAASEAIPYAKTGGLADVAGALPQEFAKLGHDVI
                     LLLPRYREVNRSGQTFRPVVTLPVQTPIGMMNAVVEEALVPVGERRMRVWTIGHDPYF
                     DRTGLYQEAGVDYPDNLERFAFFCRAIIETMV"
     gene            complement(422..883)
                     /locus_tag="VFH05_13270"
     CDS             complement(422..883)
                     /locus_tag="VFH05_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679241.1"
                     /translation="MTLRRRALGVGLIAVYGLLVMSGCTGLSLAEQEQKIRNNDLILR
                     KLEPRAFVRAWGMPTYQRMEFMPFFGMKDGSLVPRSRLPVGASPPGWEAGVDAGEALF
                     LAYPDRGWLIVFLDETFVYREELSPDKLHEIGRTWAHEDKFRTKIELQPAP"
     gene            complement(880..1236)
                     /locus_tag="VFH05_13275"
     CDS             complement(880..1236)
                     /locus_tag="VFH05_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET6679242.1"
                     /translation="MAETQLEPRDLSWLDKGVLGIAWSDGHNGIYPVRHLRLHCPCAA
                     CVDEWTGVRRLKADDVPLLIMLQDIEPVGRYALQFKWSDGHDTGIYSYKLLRELCQCD
                     VCQPVKTNEPKSRRLL"
     gene            complement(1268..2020)
                     /gene="recO"
                     /locus_tag="VFH05_13280"
     CDS             complement(1268..2020)
                     /gene="recO"
                     /locus_tag="VFH05_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET6679243.1"
                     /translation="MPLVKTPAITLKSRKWGEADRIVTFYAKHLGKIRAVARGARRLK
                     SRMGAALEPLTLCELNLFEKSGDSLYRVSQVDLVEPFVRFRENLTLMAAAARMTNVVA
                     AVTPDGDPDPHLFETLEQGLRTLVTSEDPALTALLFQIRLLGLTGFRPQTDQCAACGK
                     TSVINEPQFSPASGGLVCALCAARQRVPCLPLSRGSLAFLHQALQLAPAMVTRLKAAG
                     QVRCEVEDAIERYVTVVAGRRIPPVDFLSQPL"
     gene            complement(2062..2208)
                     /locus_tag="VFH05_13285"
     CDS             complement(2062..2208)
                     /locus_tag="VFH05_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET6679244.1"
                     /translation="MATFMPIVLKRMGVDPAVAAGPFVTTANDITGITIYLTLATIFM
                     EYLR"
BASE COUNT          504 a          676 c          587 g          442 t
ORIGIN      
        1 acaccatggt ttcgatgatc gcccggcaaa agaacgcaaa ccgctccaga ttgtcaggat
       61 agtcgacccc agcttcttgg tagagaccgg ttcgatcaaa gtagggatca tgcccgatcg
      121 tccacacgcg catgcgccgc tctccgacgg gcacaagcgc ttcctcaacg actgcattca
      181 tcatccctat cggggtctgg accggtagag tcaccacagg tctgaaggtc tgaccagaac
      241 gattcacttc ccggtatcga ggaagcagga gaatgacgtc atggccgagt ttggcgaact
      301 cttgcggcag ggcgcccgcc acgtccgcaa gcccacccgt tttggcgtaa gggatggctt
      361 cagaggcggc aaccaagaga ttgaacggac gggaacgttt caccacggtc ctgatggcga
      421 gttatggagc aggttgtaat tcgatcttgg tccgaaactt gtcttcatgc gcccatgtcc
      481 gcccaatttc atggagcttg tccggcgaaa gttcttcgcg atacacaaag gtttcatcca
      541 gaaagacgat cagccagcct cggtcaggat aggccaaaaa taaggcctct ccggcgtcaa
      601 cccccgcttc ccatccgggc ggtgacgccc cgaccggcaa tctcgagcga gggacgagcg
      661 acccgtcctt catgccaaaa aacggcatga actccattcg ctgataggtc ggcattcccc
      721 aggcacgaac aaacgcccgc ggctccaatt ttctcaggat gagatcattg ttccgaatct
      781 tctgttcttg ttccgccaac gacaggccgg tacatcctga catcactagc aagccataga
      841 cggcgatcaa tcccacaccg agagcacggc gtcggagcgt cataggagtc tccggctttt
      901 tggttcattc gtcttcaccg gctggcacac gtcgcactgg cagagttcgc gcagcagctt
      961 ataggagtaa ataccggtat cgtgcccgtc gctccatttg aactgcaatg cataccggcc
     1021 gaccggttcg atgtcctgca acatgatgag cagcggcaca tcgtcggctt tgagcctccg
     1081 cactcctgtc cattcatcga cacaagccgc acaggggcaa tgcaagcgca gatggcgcac
     1141 cggatagatg ccgttgtgcc catcgctcca agcgattccc aatactcctt tgtcgagcca
     1201 ggataagtcg cgtggttcaa gctgcgtttc tgccatgttc actggtatcc tctgagaatc
     1261 tgacgcctca cagcggttgc gaaagaaaat cgacaggagg aatccgtctt cctgcgacca
     1321 ccgtcacgta cctctcgatg gcatcttcga cttcacaacg gacctggccg gctgccttga
     1381 ggcgggtgac catggccggc gccaactgca acgcctggtg gagaaacgct aaactccctc
     1441 gcgataacgg cagacagggc acccgctgcc gtgcggcaca gagcgcacac accagcccac
     1501 cggatgcagg agaaaactgc ggctcgttaa tcacgctcgt ctttccacaa gcagcacact
     1561 gatcagtttg cggacggaag ccggtcaaac cgagcagtcg aatctgaaac agtagcgcag
     1621 tgagtgctgg gtcctcactg gtcaccaaag tgcgaagtcc ctgctccaag gtctcgaaca
     1681 gatgcgggtc aggatctccg tccggcgtga cggctgccac cacattcgtc atccgagcgg
     1741 ccgccgccat cagcgtcaaa ttctcgcgga acctcacgaa cggttcaacc aaatccacct
     1801 gcgacacccg atacaacgaa tcgcctgact tctcaaacag attcaactcg catagcgtca
     1861 acggttcaag ggcagctccc atccggcttt tgagtcgccg cgcaccgcgt gccaccgcac
     1921 gaatcttccc cagatgcttc gcatagaagg tcacgatgcg gtcggcctcg ccccacttgc
     1981 ggcttttcaa ggtgattgcc ggagtcttta cgagcggcat gccatgtccc tgtgcgaatg
     2041 tgtcagaagg cgatgagacc gctatcggag atattccatg aatatggtgg ccagcgtgag
     2101 atagatagtg atgcctgtga tatcgttcgc cgtcgtaacg aacggtccgg ccgcaacggc
     2161 aggatcaacg cccatccgtt tgagtacaat cggcataaat gtagccatg
//
LOCUS       DASSCQ010000210         5591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1659390, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000210 DASSCQ010000000
VERSION     DASSCQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659390"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1016)
                     /locus_tag="VFH05_13290"
     CDS             complement(66..1016)
                     /locus_tag="VFH05_13290"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6679245.1"
                     /translation="MKQSNVERILLATDFSDSAVLTHDYAEYLALSLSASLDILYVSE
                     SARASSGAELAGQQHVQTLLHTLQDRMKEHGVRVTARWVAGNPGAQILTAARQLDADV
                     IAMGMQGHTQVSHGLLGSTVEPVTRAGYCPVLTVPLPQKETSSCGMTSTGTIGIRRIL
                     APIDFSTPSLDSLEYAVKLAQDLAANLVLLHVLEPEHGGWNLTRMEEAERIRSSWEGR
                     LRELVEIIKSLAVSATYDIRGGQPPDSILAGALQHKCDLIVIGTHGRQGRDRANVGSV
                     AEAVLKQTACPVLTVKNPKFPSDGRRIISGALSQPNAGTH"
     gene            complement(1180..1698)
                     /locus_tag="VFH05_13295"
     CDS             complement(1180..1698)
                     /locus_tag="VFH05_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679246.1"
                     /translation="MNTQAVLALACWFCLGAGATFGESRVTAREGPSGDFLEFEGQSH
                     GQIVKHSLPLYRTGAIRYFSAGVGSEERQAEYPPFSLKLVFTAGGKPYLVGVDVLIQS
                     VKGQAITISRDQVEGPWLFVDLPSGMYDISASYGEHKQALKGIKVVAGRQKTIHIRWA
                     DEAGASVKVPPQ"
     gene            2013..2918
                     /locus_tag="VFH05_13300"
     CDS             2013..2918
                     /locus_tag="VFH05_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6679247.1"
                     /translation="MRILCAVDGSDYSKWGVQMLEALAGREPEHVTLLHVINKPRPGA
                     TQGTMPRAEKQALASMEKAGELLLRDAERSAKGALAQAMTGPRTALHQVLTYGSIAPT
                     IAQQARRLRADLILMGSRGLSDIKGFLLGSVSRQVVSMAPCSVLVVKERCAVLRRVTL
                     AVDDSKPSRAAAQFLRSQILPPSAMVTILSSMESPVTDLAARYLSSSQLAELTEPVRV
                     RTLNLVNSFRDAFIKEGLSVTTAVHMDHVIDTIVKHVEAEDADLLVVGSRRLTKTERR
                     YLGSVSESLLRHAPCSVLIVRGARA"
     gene            2911..>5591
                     /locus_tag="VFH05_13305"
     CDS             2911..>5591
                     /locus_tag="VFH05_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET6679248.1"
                     /translation="MPDFRYHDINRLSTADVLNRLETAGGGLTTEEAIRRLIQFGPNV
                     LAAPERYSVLRGLARQFTHFLAILLWIAAGLSFVAELMNPGEGMATLGWAILAVIGIN
                     AVFAFLQEYKAERAVQTLRRLLPDKAWITRNGQSVGIRRSELVPGDVLLLEEGERVPA
                     DARLIEEAGMRVDTAALTGESKPKRRTAEPVEDGHWLDLPNLVFAGTMILSGHGRAVV
                     FATGMRTEFGKIADLTATVETGLSPLQKEIVKVTRIVAAISLAMGITFFGIGLLTGLS
                     FWISAIFGIGIIVANVPEGLLPTVTLSLAMASQRMAKRHALIKHLASVETLGCTTVIC
                     TDKTGTLTENRMKVDRFYVDGLVIESREGCFFTRGRIVSTTDAEQWRFFFDVLLHCHN
                     AKRVRQADGRYVVTGDPTEVALLQFAIEHGLAHRPPLRRMGELAFDADRKRMSTLHWS
                     EGQLIAFTKGAPESVVPLCTHIEIHGNAVPMTSDERARILSQSRSFAEQAYRVLAVAR
                     REVANQPEHIEVETIEADLTFLGLIAMMDPPHREVPGAVARCRNAGVRVIMITGDHPL
                     TALAVARKIGLVPHGIAQPPVGFVPVIEGLQVERMSDEYLRQLLTPSRSNEPEPVFAR
                     MAPRHKMRVVSILKEMGEVVAVTGDGVNDAPALKKADIGIAMGVAGTDVAKEIADIIL
                     LNDNFATIVSAIEEGRAVFANIRKFATYVLVSNVPEVVPFLGYGFFGIPLALTIPQVL
                     AVDLGTDMVPAIALGAEPPHPGIMDVPPRPRTERLLSTPLLLRAYLFLGLNSALLAMG
                     GFFLYLFANGWTWGVHLEWSSPLLQSATTVTLAGIVLAQVANVFACRSARLSAFRVGW
                     LTNPLIYWGIAVELTLFAMISYTSIGNQMFGTAPLPL"
BASE COUNT         1146 a         1612 c         1613 g         1220 t
ORIGIN      
        1 atgtgaagaa tgttatcaga ccgggtagaa cgccacttcc tcgccatccg tctcgaggcc
       61 attgttcagt gtgtgcctgc gttcggctgg gacaacgcgc cggagattat gcgacggccg
      121 tctgaaggga attttggatt cttgaccgtc agtacggggc aagcggtttg cttgagcacg
      181 gcttcggcaa cgcttccgac atttgcacga tcccgcccct gccgtccatg cgtaccgatg
      241 acaatgagat cacacttgtg ttgaagcgcc ccggcgagaa tggaatcagg tggctgccca
      301 ccacgaatgt cataggtggc cgatactgca agagacttga tgatctcgac caattcccgt
      361 agccgacctt cccacgagga gcgtatccgt tcggcctctt ccatccgagt gagattccag
      421 cctccatgct ctggttccaa cacatgcaac aagacgagat ttgcggctag gtcctgagcc
      481 aatttgaccg catactccaa ggaatccaag gagggcgttg agaaatcaat cggtgccaga
      541 atacgccgaa ttccaattgt gcccgtcgag gtcataccgc aggaactcgt ctccttctga
      601 ggcaacggga cagtgaggac cggacagtat ccggcccgtg tgaccggttc gacggtactg
      661 cccagcaatc catgagatac ctgtgtgtga ccctgcatac ccattgcgat cacatcggca
      721 tcgagttgtc gtgctgcggt caagatctgc gcaccgggat tccctgccac ccatcgagcg
      781 gtgacgcgca ctccgtgttc tttcatcctg tcctgtaacg tatgcaggag agtctggaca
      841 tgctgctgac ccgctagctc ggctccggag gacgcccgag cggattcgct gacatacagg
      901 atgtccaacg aagcgcttaa gctcagcgcg aggtactcag catagtcatg tgtgaggaca
      961 gcgctgtccg aaaagtccgt tgccagtaag attctctcta cgttggattg cttcatgcgt
     1021 cgcttccgaa tgatggtcgt gcacttcaga gaagtcttac tctgtcccag actctatgat
     1081 ggtgtcaaac cgttaggctt tggccgactt cgtacggctc gtcattgttt ttatagcatc
     1141 tcgccctttc tgtcgacacc aacaaattgg cgccaatttc tattgcggtg ggaccttcac
     1201 tgatgcgccg gcctcatccg cccaccgaat atgaatggtt ttctgtctgc cggcaacgac
     1261 cttgatgccc ttcagcgcct gtttatgctc gccatacgac gctgaaatgt catacatgcc
     1321 gctgggaaga tccacaaaaa gccaggggcc ctcgacctgg tcgcgtgaga tggtaatggc
     1381 ctgtcctttt accgactgaa tcaacacgtc gacgccgacg aggtaaggtt tccctcccgc
     1441 cgtgaatacc agcttcaacg agaacggcgg gtattccgcc tgccgttcct ccgatcctac
     1501 accggcgctg aaataacgga tcgctccagt ccgatagagt gggagggagt gtttgacgat
     1561 ctgcccatga ctttggccct caaattccag gaagtcaccg gaaggccctt cgcgagctgt
     1621 gacgcgagat tctccgaagg tggctccggc acccaggcag aaccagcatg ccaatgccaa
     1681 gacggcctgt gtattcaacg taagacctcc cggacggcat acctcgcaat cacggtgcca
     1741 atctgattgg tgagcaaaaa tccgaacttc ccctgcattg cgagacttct aattctgtac
     1801 catcaatcaa tagaagcagg actgacgcta atcaccccac tcagtctttt catgctgctg
     1861 caaaacgcag catcagcaga tcacaaactc gcctgattcc tctaagccct tggattgggg
     1921 ccattcatgc tccagatgat gatcgactcc aggcacatcg aatgcaaccg atgtgctatg
     1981 gatggaagcg gtctgatgag gagaaagccg agatgcgaat tctttgtgcg gtagatgggt
     2041 cggactattc gaagtggggt gtccagatgc tggaggcgct cgcagggcgg gagccggagc
     2101 atgtgaccct gctccatgtg atcaacaagc cccggccggg tgcgacacag ggaacaatgc
     2161 cgcgtgctga gaagcaggca ctggcctcga tggaaaaggc aggcgagctc ctgctgcgcg
     2221 acgcagaacg gtcggcgaaa ggcgcgcttg cacaggctat gaccggtcca aggacagcgc
     2281 tccatcaggt cctgacttac ggttccatcg cgccgacgat cgcgcaacag gcgcgccggc
     2341 tgcgagccga tctcatcctg atggggtcaa gagggttgag cgacatcaag ggcttcttgt
     2401 taggcagtgt ctcgcggcag gtcgtgtcta tggctccgtg ctcggttctg gtcgtcaaag
     2461 aacggtgtgc agtgctccgg cgtgtcacgc tcgccgtcga cgattcgaag ccatcgcgag
     2521 ccgccgcgca gtttcttcgc tcccagattc ttcccccgtc agcgatggtg accattctct
     2581 cttcgatgga gagcccggtt acggatttgg cggcacgtta cctatcgtca tcgcaacttg
     2641 ccgaactcac agaaccggtg agggtgcgaa cactcaactt ggtcaactct ttccgtgacg
     2701 ccttcatcaa agaaggcctg agcgtcacca cagcggtgca catggatcat gtcattgata
     2761 cgatcgtcaa gcacgttgag gcagaggatg cggacctgct tgtcgtcggc tcgcgcagat
     2821 tgaccaagac tgagcggcga tatcttggaa gcgtgtcaga aagcctattg agacacgccc
     2881 cttgctcggt gttgatcgtg cggggtgcgc gtgcctgact tcaggtatca cgacatcaac
     2941 cgactctcga cggcagatgt gctgaatcgg cttgaaacag ccggcggcgg tctcaccact
     3001 gaggaagcga tacgccggtt gatacagttc ggtcccaacg tcctggcggc accagagcgg
     3061 tattcagtgc ttcggggatt ggctcgccag ttcacgcact ttctcgccat cttgctctgg
     3121 atcgccgccg gtctctcatt cgtggccgag ctcatgaacc cgggcgaagg catggccaca
     3181 ttgggctggg ccatcctggc tgttatcggg attaacgctg tgttcgcgtt cctgcaggaa
     3241 tataaggcgg aacgagctgt tcaaacgctc cgccgtttat tgccggacaa agcgtggatt
     3301 acacgcaacg ggcaatcggt tgggattcgg agaagcgagc ttgtgccggg tgacgtgctg
     3361 ctcttagaag agggagaacg ggtaccagct gacgcgcgcc tcatcgagga ggcggggatg
     3421 cgcgttgaca cggctgcgtt gacaggagaa tccaaaccga aacgacgcac tgccgagccg
     3481 gtagaggacg gacattggct cgatctcccg aacctcgtgt tcgcgggaac gatgatcctt
     3541 tccggacatg gtcgcgccgt cgtatttgcg accggcatgc gcacggaatt tggcaagatt
     3601 gccgatctca ccgccacggt ggagacaggc ctgagtccgc ttcagaaaga gatcgtgaag
     3661 gtcacccgca ttgtcgctgc catctccctg gcgatgggca tcaccttttt cggtatcgga
     3721 ctattgaccg gattgagctt ttggatcagc gccattttcg gcatcggcat catcgtcgcc
     3781 aacgttccag agggactact tccgaccgtg acattgtcgc tcgcaatggc cagtcaacgc
     3841 atggcaaagc gtcacgcgtt gatcaaacac ctggcttccg tcgaaaccct cggctgtacg
     3901 acggtgattt gcacggacaa gacggggact ctgacggaaa accgcatgaa ggtggatcga
     3961 ttttacgtcg acggcctcgt catcgaatca cgcgaaggct gcttcttcac gcggggccgc
     4021 atcgtcagca cgaccgacgc cgagcagtgg cggttctttt ttgacgtcct gttgcattgt
     4081 cacaatgcca agcgtgtccg acaggccgac ggtcggtatg tcgtgacagg agacccgaca
     4141 gaagttgcct tgctccaatt tgcgatcgaa catggattgg cgcatcggcc tcctttgcgc
     4201 cgcatgggcg aactcgcctt tgacgccgat aggaagcgaa tgagcaccct gcattggtcg
     4261 gaaggccagc tgatcgcctt tacgaagggc gcaccggagt ccgttgtccc gctctgcacc
     4321 catattgaga tacatggaaa tgccgtgccg atgacctccg atgagcgcgc ccgcatcctg
     4381 agtcagagcc ggtccttcgc ggagcaggcc tatcgagttc tggctgtggc caggcgggaa
     4441 gtggccaacc agccggaaca tatcgaggtg gagaccattg aagcggacct cacattcctc
     4501 gggcttatcg ccatgatgga cccaccgcac cgagaagtgc cgggggccgt ggccaggtgc
     4561 cggaacgccg gtgtgcgcgt gatcatgatc acgggggacc accccctcac cgcgctggcc
     4621 gtcgcacgca agattggatt ggtgccgcat ggcatcgcac agccgcctgt cggctttgtc
     4681 cccgtgattg agggcttgca agtggaacgc atgagcgatg agtatctacg ccagttgctc
     4741 actccttctc gttccaatga accggagccc gtgtttgccc gaatggctcc tcggcacaaa
     4801 atgcgtgttg tctcgatctt gaaagagatg ggcgaagtgg tagcggtgac cggtgacggc
     4861 gtcaacgatg cgccggcgct caagaaggcg gacatcggaa tcgccatggg ggtcgccggt
     4921 acggacgtcg ccaaagagat cgccgatatt attctgctca acgataactt tgcgaccatc
     4981 gtcagcgcga ttgaagaagg acgcgccgtg tttgccaaca ttcggaaatt tgcgacctat
     5041 gtgctcgtca gcaacgtccc ggaagtggtc ccatttttag gctatggatt tttcggcatc
     5101 ccgcttgcgc tgacgatccc tcaagtcctg gcggtcgatc ttggaaccga catggtgccg
     5161 gccatcgcac tcggagcgga acccccccat cccggtatca tggatgttcc gccgcggcca
     5221 cgtaccgagc gcctcttgag cacaccgctt ctcctgagag cctatttgtt cttaggcctg
     5281 aattcggcgc tgctggcgat gggaggattc tttctgtatc tcttcgcgaa cggttggacc
     5341 tggggcgttc acctggaatg gtcctctcca ttgttacaga gcgcgaccac ggtcaccttg
     5401 gccgggatcg tgttggcgca ggtcgcgaat gtcttcgcct gccgttccgc ccgcctctcg
     5461 gccttcagag tcggctggct taccaacccg ttaatttatt ggggaatagc ggtcgaattg
     5521 accctgttcg caatgatcag ctacacctcg atcggcaacc agatgttcgg aaccgcaccg
     5581 ttgcctttgt g
//
LOCUS       DASSCQ010000211         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1182057, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000211 DASSCQ010000000
VERSION     DASSCQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182057"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFH05_13310"
     CDS             <1..408
                     /locus_tag="VFH05_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679249.1"
                     /translation="TRLDAGSGNESRLVSAGAILINGGELFIAQQSVISAQATLGGRI
                     LVQACNIALTDSQFITSTGGANPYTTAGQITLGGKSITLTNGQILSGAYGGSGGTIDI
                     TTPVLQRNGSSVIDASSTLGTDGTVTINGIIQP"
     gene            complement(668..742)
                     /locus_tag="VFH05_13315"
     tRNA            complement(668..742)
                     /locus_tag="VFH05_13315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(708..710),aa:Gly,seq:ccc)
     gene            867..1181
                     /locus_tag="VFH05_13320"
     CDS             867..1181
                     /locus_tag="VFH05_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679250.1"
                     /translation="MARQPLLLLLITTFIGGLVGGMLGEWLSLSGEVHAQKTNSVNAE
                     EFLLIDQNGRARAGLGLDPNGEVGLVLTSRDGSRTLALSPDGPVAVKLTDRSGRVLWS
                     AP"
     gene            complement(1268..1459)
                     /gene="rpmB"
                     /locus_tag="VFH05_13325"
     CDS             complement(1268..1459)
                     /gene="rpmB"
                     /locus_tag="VFH05_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET6679251.1"
                     /translation="MAFVCEICSKKHQSGHNVSHANNKTKRTFNPNLQRVRALIDGSA
                     RRIRVCTRCLRSGKVKKAL"
     gene            complement(1582..2997)
                     /locus_tag="VFH05_13330"
     CDS             complement(1582..2997)
                     /locus_tag="VFH05_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679252.1"
                     /translation="MKHLVIAIIILWAAFHFGGMFFSAKDRSPDVAFGRLLDAPQRSI
                     QNPYHNGYFYLIGLMAASPLDPGKTGYEIWVEETEDAGRGASHERKATRADLVFTLSS
                     DTTSSAWEADDPLHAFGKKEAPFRAAANQYHILLARYERWLGMPFDDWGFGRKVVPLG
                     KHILAIHRLYVADGFSVGTMQGLARLEKELQSWRTVLREAKTIDTKVLAQVLITDDLQ
                     LLSRMLSKPVVDKAILAMGLQLTLPLSTAEYSLRWPVRHQVALAVREGSPRSRSDLQQ
                     THQSSNHDWLTEAAHLPIYAFKSIEHPPAHSTMGVFQFGQASNAYAAYYEAIIMASES
                     HSQYLPRMQEVVGTLDRGRLESMLTPRLIEPSWEIFHGELIETDTRFRLASLQIQLRR
                     PSSQTAVPTRLAEVGSQYFDPFTGLPMLWSPTQQKLYSVGKDRLDDGGDPTFDISVPA
                     AVAPTIVKTSASPATTHSRRR"
     regulatory      3203..3398
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3792..>4055)
                     /locus_tag="VFH05_13335"
     CDS             complement(3792..>4055)
                     /locus_tag="VFH05_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HET6679253.1"
                     /translation="DSLIVCKFLRKCFTDFYGEAAEILRGVTGWDYTPAELRCTGERI
                     HTLKKVFNIRERWAPARELDGEPWRNTAFALNHPQTFGARCWP"
BASE COUNT          853 a         1092 c         1215 g          895 t
ORIGIN      
        1 accagactcg atgcaggcag tggaaatgaa agccgccttg tgtcagccgg tgccattctc
       61 atcaatgggg gagagttgtt tatcgctcaa cagagcgtca tatcggcaca agcgacactt
      121 ggtggccgca tcctggtgca ggcctgtaat atcgcactaa ctgacagtca gttcattacc
      181 tcgacgggcg gcgcgaatcc ctatacaaca gcaggccaga ttaccctggg tgggaagagc
      241 atcacgctga cgaacggtca gattcttagc ggggcgtatg gtgggtccgg cggcactatt
      301 gacatcacga cccctgtcct ccagcggaat ggcagtagcg tgattgacgc ttcttccacg
      361 ctcggcacag acggcacagt gacaataaat gggatcattc aaccttaggg acatcggcgc
      421 caacgctctg tttagccatg gtccctcata atccgctgtg aggtggaatc gtgtgcttca
      481 gtatcgacac ctagaggctc gcgtatagct gctgaaatac atcgatgaga gatgatatct
      541 tcgacgcgac ccgtttgact gccatgcaga tcgtttaatt tgagaaggaa tacatgcagg
      601 gtgtgtttcg gccccggcaa tcaagaggct tcgcctgaag agccgccggt acacacgaag
      661 tgtggtttgg agcgggcgat gggatttgaa cccacgacaa ctagcttggg aagctaggac
      721 tctaccactg agctacgccc gctcgggaac agaaggaaga atcctacgat ggctccgccg
      781 gcaagtcaag gaacgcgttt ccaggataga ggcgtttcta attgaagtgc acgaccatgc
      841 gctcgaaggt gcagaaagga atcatcatgg ccagacagcc gctacttctg ctgctgatta
      901 ctacattcat tggcggcctc gttggcggga tgctgggtga gtggctctct ctgtcggggg
      961 aggtgcacgc ccagaaaacg aacagcgtga acgccgaaga gttcctgctc atcgatcaaa
     1021 acggacgggc gcgggcaggg ctgggtttgg acccgaacgg ggaggtcgga ctcgtcttga
     1081 ccagtcggga cggcagcaga acgttggctt tgtccccgga tgggccggtt gccgtaaagc
     1141 tcactgatcg gtccggacgt gtcttgtggt cggcaccgtg aagtggccag cgtggtggca
     1201 aagaccaaga agtcgcctga gtagtagcgc ctgcgcttcc gtaacggtgc tgtgtgggag
     1261 gagtccgcta gagagctttt ttgactttgc cgcttcggag gcagcgggtg cacacacgga
     1321 tacgtctggc cgatccgtca atcagcgcgc ggacgcgctg caggttgggg ttgaacgtcc
     1381 gtttcgtctt gttattggcg tgactgacgt tatgtcccga ctggtgcttt ttggagcaga
     1441 tctcacatac gaatgccacg aggagactcc ttcgcaagaa agtgtgattg cgcgtgactc
     1501 tagcataggg tattcagcat tgacaagtct gcccgccctg caagtctttt gaacctagcg
     1561 ttccgcaggc gtgaatgaca ctcatcggcg tctggagtgc gtggtcgctg gactcgcaga
     1621 ggttttgaca atggttgggg ccaccgctgc gggaacgctg atgtcgaacg tcggatcacc
     1681 accgtcatcg aggcggtcct tccctacgct atacagtttc tgctgagttg gactccataa
     1741 cattggcaat cctgtaaacg gatcgaaata ttgcgacccg acttccgcta accgcgtagg
     1801 gaccgccgtt tgcgagcttg ggcgccgaag ttggatctgc agcgaagcca gacggaagcg
     1861 agtatcggtt tcgatcagtt caccgtgaaa gatttcccag ctcggttcga tcagacgtgg
     1921 agtgagcatg ctctcgagtc gtccgcgatc cagcgtcccg accacctcct gcatccgtgg
     1981 cagatattgt gagtggctct cggacgccat gatgatggct tcgtaatagg cggcatacgc
     2041 atttgaggct tgtccgaatt gaaagacgcc cattgtggaa tgcgccggtg ggtgttcaat
     2101 gcttttgaat gcatagatcg ggagatgggc cgcctctgtg agccaatcat ggtttgaact
     2161 ttgatgcgtc tgctggagat cgcttctgct acgcggggag ccctccctca cggccaatgc
     2221 cacttggtgt ctgacgggcc agcggagcga atactcggcg gtggatagcg gaagcgtgag
     2281 ctgcaagccc atggccagga tggccttgtc gaccaccggt ttcgataaca tgcgcgacag
     2341 cagctgcaag tcgtcggtga tcagaacctg tgccaacacc ttcgtgtcga tcgtttttgc
     2401 ctcacgaagt accgtgcgcc atgactgcag ttctttttcc agccgcgcga gcccctgcat
     2461 cgtcccgacg gaaaacccgt cggctacata cagccggtga atagccagaa tgtgcttgcc
     2521 gagcggaacg accttgcgcc caaagcccca atcatcgaac ggcatgccga gccagcgctc
     2581 atatcgcgcc agcagaatgt gatactggtt ggcggctgct cggaagggag cctctttctt
     2641 cccgaacgca tgcagcggat cgtcggcttc ccaggcggag ctggtcgtgt cggatgagag
     2701 agtgaacacg aggtcggctc gggtggcttt ccgttcgtga gatgcgcctc ggcccgcatc
     2761 ctcagtttcc tccacccaga tctcatagcc ggtttttccg gggtccagcg gagaggctgc
     2821 catgagccca atgagataaa agtacccgtt gtggtaaggg ttctgaatag accgctgcgg
     2881 cgcatcgagc aggcggccga acgcgacatc cgggctgcgg tctttggcag aaaagaacat
     2941 gccgccaaag tggaaggccg cccacaatat gatgatggca atgacgaggt gtttcatcgc
     3001 tggatccacg gtccgccggg cttcttaccg ggctcttctc tatgaaggga ggacgcgaga
     3061 gttctcccaa tacatctata agaggcaagc gctatgccgc agaaggcctc aaaaagtcgt
     3121 gccgtttcag caggttggtc ctaggaccgt gtttgtggct gtttgcagcg cgggcactcg
     3181 gacattattg aacttgacaa aaaatgccac tctctcctag agtgccgctc acaatcgtca
     3241 tattcgggta agggaagagg gtgcaaatcc ctcgcggtcc cgccgctgtc aatggggacg
     3301 aaacccgctt acgccactgt ccggttcgac ggatgggaag gcacggggag taggatgaac
     3361 catgagccag aagacctgcc cggaatggga atctttcagc ggatctctcg ccactcgcga
     3421 gtgatcctct ccttggtttc ctagaccatc gttccctcga gggctgggga acaggcgagg
     3481 atgaagaagc gggtgcaatc ctcagggtct ttttcaggcc ttggggattt tttatttgcg
     3541 cgcattgaaa acggtttcca gcggcgttct cgtttcgctt aacgcctgca acgtacggac
     3601 aaaagtacgt ttcggcgttt cgctcactgc ggccttgctg aaaagccgtt ttgaacgcac
     3661 tagccgttac aaagtggtga gtatcctcgc tctctgtacc ctattgctgg gttgtcttgc
     3721 tgctctcgcc gatgccgata cgaaagtaat tatgaagcga cgtcaacaag gcatcctgac
     3781 cggcatgccg ttcatggcca acatcgcgcc ccgaacgttt gtggatgatt cagggcgaaa
     3841 gctgtatttc gccaaggctc cccgtcgagt tctctcgctg gcgcccagcg ctcgcggatg
     3901 ttgaacacct tcttcaaggt gtggatgcgc tcgccggtac agcgcagttc ggccggggtg
     3961 tagtcccagc ccgtgacgcc gcggagaatc tccgcggcct cgccataaaa gtccgtgaaa
     4021 cacttgcgca ggaacttgca gacgatcagc gagtc
//
LOCUS       DASSCQ010000212        14576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_456264, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000212 DASSCQ010000000
VERSION     DASSCQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456264"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFH05_13340"
     CDS             complement(<1..310)
                     /locus_tag="VFH05_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate hydratase"
                     /protein_id="HET6679254.1"
                     /translation="MEKDEIRKAIKAKRLAKKVTIAEVAKAVGRNPTFVGAVLQGTHK
                     LPPEEAKKVGDLLGLDAELTSSLSKFPVRTDFPNTTDPFKYRLMEIIGVYGDSMREQA
                     N"
     gene            complement(338..1201)
                     /locus_tag="VFH05_13345"
     CDS             complement(338..1201)
                     /locus_tag="VFH05_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="This model describes the ATP binding subunits of
                     ATP-binding cassette (ABC) transporters for nitrate
                     transport, or for bicarbonate transport, in bacteria and
                     archaea; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HET6679255.1"
                     /translation="MAFLLIDHVTKYFANTSGSGQVCIFKDATIKIEKGEFVTCIGHS
                     GCGKSTLLNIIAGLETPTEGGIILNGREASGPGLDRMVVFQNFALMPWMTVFENIALA
                     VRSAYPDWSREQVSAHVTKYIQLVGLKGAENKRPVALSGGMKQRVGLARAFSIEPKVL
                     LLDEPFAQIDALTRGVIQEELIQMWNNSRNTVFMVTHDVDEAILLSDRILLMTNGPEA
                     RIAEEVHVSIPRPRSRETVIEHPHYYKIRNHIIHFLVRHAKHGVGEGITPGTNGSPVV
                     VNFEEDSLVTH"
     gene            complement(1205..2254)
                     /locus_tag="VFH05_13350"
     CDS             complement(1205..2254)
                     /locus_tag="VFH05_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6679256.1"
                     /translation="MESTDRTILPELTPVARAEPSVKLPIHWFQAGVASTKRRLMRWR
                     SASHAQRGGGMAAQSQGNPWYMAGFVLVVFLLSWHFFTLRSSFDQRGLTEEQLQMMEF
                     NGDIVRVPDGSYAWNPEKQKVSGIPGPLAVLAKAREELSEAFVKKGTNDHGIAYLVLY
                     TVTRFAGGFLAASIIAIMFGVILGLNKVLFRAANPFIQIMKPISPLAWMPLLLYTVKD
                     PKWTAVLVVFMAAVWPTLATTAFGVSSLRKEYLHVAAIVQLSWVERLFKVILPGAAPT
                     IVNGLRISFGSALVAVVPAEMLLGELGVGYLSWIEWNNLDIAGVIFAILVVGVVGVIL
                     DSGFNKLAGYVTYQE"
     gene            complement(2264..3571)
                     /locus_tag="VFH05_13355"
     CDS             complement(2264..3571)
                     /locus_tag="VFH05_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6679257.1"
                     /translation="MSDEQLSEDKTKRPSSADHYFERTLDQAVTAAVVEAIGPTRRQV
                     LLGLGAATLSALVADIFPLRTLKAFAADPVGKPEKKDVSIGFIPITCGTPIIMAEPLG
                     FYKKHGLNASVKRAAGWAMIRDWTVNKEVDAAHMLTPMPLAITLGAGSVPTPIYMPAV
                     ENINGQAITLHIKHKNVKTASEMKGFRFCVPFDFSMHNYLLRYFLAEGGVHPDKDVQI
                     RVVPPPEMVANLKAGNVDGYLGPDPFNQRAVYENVGFIYKLSREIWDRHPCCAFTVTK
                     EFATTYPNTFLAMWRAIVDATHYASDPAHRKEIASAIAPTNYLNQPVTVLEQVLTGTY
                     ADGLGNIKKEPNRIDFDPYPWHSMAIWILTQMKRWGHLKGDVNYKSVAEQVYRAADCD
                     RIAKELGYATHQSTTTRHVIMGKEFDSNNADAYVKSFKIHSMA"
     gene            complement(3992..5401)
                     /gene="glnA"
                     /locus_tag="VFH05_13360"
     CDS             complement(3992..5401)
                     /gene="glnA"
                     /locus_tag="VFH05_13360"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET6679258.1"
                     /translation="MNVREALEFAKKNRVQVVDLKFVDLIGTWQHFTIPASELTEDLF
                     KDGSGLDGSSIRGWKAINNSDMLVVPDPATACLDPFTAVPTLSLIGNVVDPISRETYE
                     RDPRYIAQKAEKYLQSTKIGDTSYWGPEAEFFIFDQARYDQTSHSGYYHIDSDEGVWN
                     MGQEGVNLGGKIRHKEGYFPVPPTDTQQDIRSEMILEMEKVGIAVEKHHHEVATAGQA
                     EIDIRFDSLLRTADKMMMYKYIVKNVARRHGKTVTFMPKPIFGDNGSGMHTHQSIWKE
                     GKPLFAGKEYAGVSQMCLHYIGGILKHAPALAAITNPTTNSYKRLTPGFEAPVLLAYS
                     SRNRSAGIRIPMYSPSPKAKRIEVRFPDPGANPYLAFSAMLMAGLDGIENKINPGEPA
                     EKDLYDLEAKEAAKIRTMPGSLDDALNNLEKDHKFLLKGGVFTEDLIEAWIGYKRSKE
                     VDPMRLRPHPFEFFLYYDV"
     gene            complement(5444..8116)
                     /gene="glnD"
                     /locus_tag="VFH05_13365"
     CDS             complement(5444..8116)
                     /gene="glnD"
                     /locus_tag="VFH05_13365"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET6679259.1"
                     /translation="MERESSSSSLSEAPSLVHVLEEHRSLIAQRVMAGVSGAETLASM
                     TEFVDGLILGRYRDALRQGGEERATVGMRQCCLMALGGYGRRELAPQSDIDLMFLFEP
                     GADKAVESLVRAVLHPLWDCGFQVGHSVRTIGDSIELAETDATVKTSMMEARFLAGSA
                     ELFQVFHRKYLRKVVFKNTDAYLDQKLEERRREYEKFGETVYLLEPNVKKSKGGLRDL
                     HLLQWAGMARYQAPTIRELSDRGILSRADLLALTEAREFLWRVRSLLHVHAGTAQEIL
                     TFDEQVWLAQHFGYHDQPHLLAVEQFMQQYYQHTMGLHERCMRFVERCRSVPFWRRMA
                     RFLPAPRVDGYFVVRGSLLSVADEHRDRVLDSPALLLHLFDLARDRNLTIVPPLIEDI
                     HRHVDQVSADAYRTPEVSRAFLRILSGPRTTGTLEAMHRAHLLEKLVPAMGTVRGLMQ
                     FNQYHKYTVDEHSLLAVGRAEALSQDTGVLGEVYRAIKRKDILHLAVLMHDLGKGREE
                     DHSDVGKRLSEEAALRLGLDEQETRTLAFLVHRHLLMAHTAFRRDPNDDKVVLPFARE
                     VGTPEVLKKLLALTAADIAAVGPGVLTKWKESLLIELYTRTMPEVSGDREVQGAPARW
                     SQVVEEVGRHMKSTGDPVADRVWIEAQLKQFPERYVYGTVPARIAAHLAAVSRLHQGD
                     VVVETDFNRPLGTCEYSVITHNELTPGIFSKITGVMAGNGLQILDAQILTRADGIVVD
                     TFQVTDPDYLGEPPADRREAVGDRIAAVLKGWEHVDEVIRRGARLRLTRPLPKAPDAT
                     EVRIDNETSDSFTIVDVFADDRQGLLHVITNAIFQLGLSIHAARISTRLDQVADVFYV
                     TDQAGKKLSDHAQLERVRAGVEQAVEQFLVIKAA"
     gene            complement(8125..8463)
                     /locus_tag="VFH05_13370"
     CDS             complement(8125..8463)
                     /locus_tag="VFH05_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET6679260.1"
                     /translation="MKLVEAIIKPFKLDEVKDALLEIGVQGMTVTEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVQDGQVPRVLETISRAAKTGSIGDGKIFVRDLSAAVRI
                     RTGETGETAL"
     gene            complement(8490..9761)
                     /locus_tag="VFH05_13375"
     CDS             complement(8490..9761)
                     /locus_tag="VFH05_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET6679261.1"
                     /translation="MSERRHWVSRGAITGLWVTVCATQAWAGQPEGSVDSGDVAWILA
                     ASALVVGMIIPGLAFYYGGLVRSKNVISTMVQAFAILCVVSVVWLVCGFTLVFGPDRG
                     GVIGGLDWALLMDVGAAPHPVYAPAVPLEAFMLFQLLFAAFTPALIAGAFAERIKFGA
                     MLLFAALWSVLVYTPIAHWLWGNGWLARIGALDFAGGAVVHVSAGFSAVACAMVVGKR
                     RGWRTDYMAPHNLPFILLGAGLLWLGWFGFNGGSARGANATAVGAVTVTHFTAVSAAL
                     AWMLAEWKHRGKPTVLGVASGAVAGLATSTAGAGMLGPLAAVALGIAAGVCSYMAIVW
                     KGKIGYDDTLDVMGTHGVGGMLGMLGVGFFAAHGHPAMQILAVVATGTFSFLGSYAIL
                     KLVDGSIGLRVTQEEESTGLDLTQHNERAYS"
     gene            complement(9824..11593)
                     /locus_tag="VFH05_13380"
     CDS             complement(9824..11593)
                     /locus_tag="VFH05_13380"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET6679262.1"
                     /translation="MRILRIAMVQMNPTVGDLDGNTRRIINWVVEARKAKPDLVAFPE
                     LAITGYPPEDLLLKPQFVADNRRALNEIVRACRHCNVVIGYVGQGRASGLREGAAAVL
                     PAGRHDLYNAAAVITDCRLALTYAKWYLPNYGVFDESRYFNPGRRLPLITVNGTVVGV
                     NVCEDIWLSEGPTRLQANAGAEVIVNINASPFHGGKSRSREEMLATRARDNRVIVTYT
                     NTVGGQDELIFDGNSVVMDHTGEPIARAKAFEEDLLVVDLNVESVARARAAEGRRPDV
                     RGRTPTAIDRVTLKGKPPARRTRLIPSIEPLADPLDEVYRALTLGVRDYVTKNGFKRV
                     VIGLSGGIDSALTAVLAVDALGPRNVLGVFMPSPYTSRESYEDTQELARRLRIQVRTL
                     PITPTFKSYLETLKPVFAGTKVDTTEENLQARIRGNLLMALSNKFGHLVLTTGNKSEM
                     SVGYATLYGDMAGGFAVIKDVPKTMVYALADRRNRQSAVIPKRTLHRPPTAELRADQK
                     DEDSLPPYPVLDPILKAYVEDDRSVDEMVGMGFARKTVLRVVSLVDRSEYKRRQAPVG
                     IKITHRGLGKDRRMPITNGYRGT"
     gene            complement(11992..12903)
                     /locus_tag="VFH05_13385"
     CDS             complement(11992..12903)
                     /locus_tag="VFH05_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET6679263.1"
                     /translation="MTTITKGARGSRAASSRSDSEGRTASIGRVGTLALEYVRRGDRT
                     IFGRTSCQTPWHLFPPMYLDDSGSAYTLLVNPSGGLVGGDRLSIDVSVGPKGHALIST
                     PSANRVYRSLSEDSVQEVTLKVGDGGIVEWIPEHTIPFAGSRFRQTIEVKLAARATIV
                     LWDAIASGRIAHGERWKFTTLSNRIHIESASGSSLAERYLIVPGNKWSGVGLAERWDY
                     IGSLFVVGDAITGTLRASLEADLADIVEKQPDQAILGGVSQPAASGLVVKLMSQSAPA
                     MTLALAELWGAVRGLLWQLPPLALRKY"
     gene            complement(12887..13558)
                     /gene="ureG"
                     /locus_tag="VFH05_13390"
     CDS             complement(12887..13558)
                     /gene="ureG"
                     /locus_tag="VFH05_13390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET6679264.1"
                     /translation="MHRADDQYCGSGRVTVARSKQIPVIGIGGPVGSGKTALVEALCR
                     TLRDRFSLAVVTNDIFTKEDAEFLTRRGALPQDRILGVETGGCPHTAIREDASHNQEA
                     LDELLRRHPEVELMFVESGGDNLAATFSPELVDHTIYVIDVAEGDKIPRKGGPGITRS
                     DLLVINKIDLAPYVGADLGVMDRDSRRMRGDRPFVFTNLMSGDGLDRVVSWVESHIHP
                     HDHHH"
     gene            complement(13634..14320)
                     /locus_tag="VFH05_13395"
     CDS             complement(13634..14320)
                     /locus_tag="VFH05_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HET6679265.1"
                     /translation="MDTLSLIQGLRFVDSFFPSGGFAFSSGLEAAVKCGAVHNGKDLA
                     GYVEDLLVQGIAKREAVGVAAGHLAATTDRLDLALAVDRQLDELKLGRDSRLASRQIG
                     RQVMKGAAGQTNENRVVKEFRRAIDEGRTPGHVPVAIGMTLAVCGWSRQDTVAAFLYQ
                     TVQGFVSAAMKLLPVGQQEGQQLLHDWGPLITELSRRAKPQTRLVSWSPVHDIYAMRH
                     ARLESRLFRS"
     gene            complement(14363..>14576)
                     /locus_tag="VFH05_13400"
     CDS             complement(14363..>14576)
                     /locus_tag="VFH05_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679266.1"
                     /translation="ERDVPHQLGLQRRIVAVKGCRTVHKRDLKLNDALPQIEVDPETY
                     VVTADGERLTCEPIIVQPMAQRYFLF"
BASE COUNT         2975 a         4552 c         3984 g         3065 t
ORIGIN      
        1 cgttcgcctg ctcacgcatc gagtcaccgt agacaccgat aatctccatc aaacggtatt
       61 taaacggatc ggtcgtgtta gggaaatccg tccgaacggg aaacttgctc aacgatgagg
      121 tcaattccgc atccaatccc aagagatcac cgaccttttt ggcttcttcg ggcggcagct
      181 tatgcgtccc ttgtagaacc gctcctacga acgtgggatt ccgaccgacg gctttggcca
      241 cctctgcgat cgtcactttt ttggcaagtc gcttggcttt aatcgccttc cggatctcgt
      301 ccttctccat gccgcccctc ctgtctgtgc gagcccttca gtgagttacg agagaatctt
      361 cttcgaaatt gaccaccacg gggctgccat tggtcccagg tgtgatgccc tctccgacgc
      421 catgtttggc atggcggacg aggaagtgga tgatgtggtt tctgattttg taataatgcg
      481 ggtgttcgat gaccgtttcc ctcgatcgcg gacgcggaat cgacacgtgg acttcctcgg
      541 caatccgcgc ctcaggtccg ttcgtcatga gcaggattcg atcggacaag aggattgcct
      601 cgtccacgtc gtgggtgacc atgaagacgg tatttctcga attgttccac atttgaatca
      661 attcttcctg aatgactccc cgcgtcaagg catcgatctg cgcgaacggt tcgtccagca
      721 gcaacacttt aggttcaatg gaaaacgccc tggcgagacc cacgcgctgt ttcatcccac
      781 ccgataacgc cacgggccgc ttgttctccg ctcccttgag acccaccaat tgaatgtact
      841 tggtcacgtg ggcgctcact tgctcacggc tccagtcggg ataggcggag cggacggcca
      901 aggcaatgtt ctcaaagacg gtcatccacg gcatgagcgc aaaattctgg aagacgacca
      961 tgcggtcgag gcccggtccg ctcgcctccc ggccattgag aatgattccg ccttccgtcg
     1021 gcgtctccaa cccggcaatg atattgagca aggtactttt accgcagccg gaatggccga
     1081 tgcaggtcac gaactccccc ttttcaatct tgatggtggc atctttaaag atgcacacct
     1141 gcccggagcc ggaggtattc gcgaaatact tggtcacgtg gtcgataagc aaaaatgcca
     1201 tggattactc ctgataggtg acatatccgg ccagtttatt gaatccggaa tcgaggatga
     1261 cgcccacgac tcccacgacc aggatagcga aaatcacgcc tgcaatgtcg agattgttcc
     1321 attcgatcca actcaaatat ccgacaccga gttcgccaag cagcatttct gcaggcacca
     1381 cggcgaccag cgcactgccg aaggagattc gcagcccgtt cacgatggtt ggagcggctc
     1441 ccggcaggat gaccttaaat agccgctcga cccacgacag ctgcacgatc gccgccacgt
     1501 gcagatactc ttttctcaag gaactgaccc cgaacgccgt cgtggccagg gtgggccata
     1561 ccgccgccat gaagacgacc agcacggcgg tccacttggg atccttgacc gtgtagagca
     1621 gcagcggcat ccaggccaag ggagaaatcg gcttcatgat ctggatgaat ggattggcgg
     1681 ctcggaacag caccttgttg aggcccagga tgacaccgaa catgatggcg atgatggagg
     1741 ctgccagaaa tcccccggca aaccgcgtta cggtgtacaa caccaaatag gcgatcccat
     1801 gatcgttcgt gccttttttg acaaaagcct cggacaattc ctcgcgcgct tttgcgagca
     1861 cggccaaagg cccggggatc ccgctaacct tctgcttctc cggattccag gcatagcttc
     1921 catcgggaac tcgcacgatg tcgccgttaa attccatcat ctggagttgc tcttcggtca
     1981 gtcccctctg atcaaaagat gaccgcaagg tgaagaaatg ccagctgagc aggaacacca
     2041 caagcacgaa tcctgccata taccacgggt ttccttgcga ctgagcagcc atccctcctc
     2101 ctcgctgagc gtgtgaggcg cttcgccacc gcatcagccg tcgcttcgtc gaagcgactc
     2161 ccgcttggaa ccagtggatc ggaagcttca ccgatggttc ggcgcgtgcg accggtgtca
     2221 gctcaggcaa tatcgtgcga tcagtcgatt ccatgaggtc tcctcaggcc atgctatgaa
     2281 ttttgaaact cttcacgtaa gcgtccgcat tgttggaatc aaattctttc cccatgatca
     2341 catgtctggt ggtcgtcgat tgatgggtcg cgtagccgag ttctttggcg atgcggtcgc
     2401 aatccgctgc ccgatagacc tgctcggcca ccgacttata gttcacgtcc cctttcaggt
     2461 gtccccaccg tttcatttga gtcaggatcc atatcgccat cgaatgccag ggatacggat
     2521 cgaagtcgat gcggttcggt tctttcttga tgttgcccag cccgtccgca taggtacccg
     2581 tcaacacctg ctccagcacg gtgaccggtt gattcaggta gttcgtcggc gcgatggccg
     2641 acgcaatttc cttccggtgc gcgggatcgg acgcataatg cgtggcgtcg acaattgccc
     2701 gccacatggc gaggaacgtg ttgggatacg ttgttgcaaa ttctttggtc accgtaaagg
     2761 cgcagcacgg atgccggtcc caaatctccc gcgataattt gtaaatgaaa cccacatttt
     2821 catagaccgc ccgttggttg aacggatcgg gtcccagata cccgtcgacg ttgccagcct
     2881 tgagattggc gaccatctcg ggcggcggaa cgacccgaat ctgcacgtct ttatcaggat
     2941 ggacgccgcc ctccgcgaga aaataccgta ggaggtagtt atgcatcgaa aaatcgaacg
     3001 ggacacaaaa ccgaaagccc ttcatctcgg atgccgtttt cacgttcttg tgtttgatgt
     3061 gcaaggtgat ggcttggccg ttgatattct cgacagcggg catgtaaatc ggcgtgggga
     3121 cggatcctgc acccaaggtg atggccagtg gcatgggagt cagcatgtgc gcggcatcga
     3181 cttccttgtt cacggtccaa tcgcgaatca tcgcccagcc ggccgcacgt ttgactgacg
     3241 cgttcagccc gtgctttttg taaaaaccca agggctcggc catgatgatc ggcgtgccgc
     3301 aggtgatggg aatgaagccg atgctcacat ccttcttttc cggcttgccc acaggatctg
     3361 ccgcaaacgc ttttaacgtc ctcaagggaa agatgtcggc gaccagcgcg ctcagcgtcg
     3421 cggcgcccaa accgaggagg acttggcggc gggtcggtcc gatggcttcg accactgcag
     3481 cggtcacggc ctgatccaat gtccgctcga aatagtgatc tgccgacgac ggcctctttg
     3541 tcttgtcctc gctcaactgt tcgtcactca aggcccgctc ctttcgtcac gaccccttcc
     3601 gccgtcaaca ccaaaacaaa aggcgtccct ctctcgctcg agagaaggac gcctttgtcc
     3661 gtcgtcagca ggtctacagc gacccgccca tcgtctattt caaaaccaca aaggcgtccg
     3721 ccattctttt ggagaatgac ggacgccttt gtccgttgcc tatcttttga agccggagca
     3781 ggcgccgttg ccttgcttag cttgttggaa cttgtagcac ggttgttccg ttgccgtcaa
     3841 ccctccgcgc cggatgctga aaaatcccgc cagcttcgtt ctcgaatcgc ttagagactc
     3901 aacgtacggc tcaaagtacg cttcgcctct gcgctcctgc ggccttgctg gacgggcttt
     3961 ttgaccatcc agcaaagctg gtacccgtct cctagacgtc gtagtacagg aagaactcaa
     4021 agggatgcgg ccgtaatcgc atggggtcaa cctctttact tcgtttgtag ccgatccagg
     4081 cttcgatcaa atcctccgta aatacaccgc ctttcagcag gaatttgtgg tccttttcca
     4141 gattgttcaa cgcgtcgtcc agactgcccg gcatcgtccg gatcttcgcg gcttccttgg
     4201 cttccagatc gtacaagtct ttttccgccg gttcaccggg gttgatcttg ttttcgatgc
     4261 cgtccaggcc ggccatcaac atggccgaaa acgccagata cggattggcg cccggatccg
     4321 gaaaccggac ctcgatgcgc ttggcctttg gactcggtga atacatcgga atgcggatgc
     4381 ccgcggaacg gttgcggctc gagtaggcca gcaagaccgg cgcttcaaat cctggcgtga
     4441 gccgcttgta agaattcgtc gtgggattgg tgatcgccgc caatgcgggc gcatgcttca
     4501 gaatgccgcc gatgtagtgc aggcacatct gcgagacacc cgcgtactct ttccccgcaa
     4561 agagcggctt cccctctttc cagatgcttt ggtgcgtgtg catgccgctg ccgttgtctc
     4621 caaagatcgg cttgggcatg aaggtaaccg ttttaccgtg acggcgtgcc acgttcttca
     4681 cgatgtactt gtacatcatc attttatccg ccgtacgcag caacgagtcg aaccggatat
     4741 cgatctcagc ctgcccggcc gtggccactt cgtggtggtg cttctcgacg gcgatgccga
     4801 ctttttccat ttccaggatc atttcgctgc gaatgtcctg ctgcgtatcg gtcggcggca
     4861 cggggaaata gccttctttg tggcggatct tgccgcccag gttgacccct tcctgtccca
     4921 tgttccagac gccttcatcg gaatcgatgt ggtaataccc gctatggctg gtctggtcgt
     4981 agcgcgcctg gtcgaatatg aagaactcgg cctcggggcc ccagtaggac gtgtcgccga
     5041 tctttgtgct ctgaagatac ttttccgcct tctgcgcgat gtaccgcgga tcgcgctcgt
     5101 aggtctcgcg tgagatcgga tcaaccacgt tgccgatgag gctcagcgtc gggaccgccg
     5161 tgaacggatc caaacaggcc gtcgccggat ccggcacgac gagcatgtcg ctattgttga
     5221 tcgccttcca accgcggatg gacgagccgt ccaaaccgga cccgtccttg aacaggtctt
     5281 ccgtcaattc gctggccggg atcgtaaaat gctgccaggt cccgatcagg tcgacgaatt
     5341 tcagatccac aacctgcacc ctgttctttt ttgcaaactc taatgcctca cgaacgttca
     5401 ttccctcctc ctttcagaca tcctgagagc cccttgcttc agcctatgcc gctttgatga
     5461 caagaaattg ttcgacagct tgttcgacgc cggcccgcac gcgttccagc tgcgcatgat
     5521 cgctgagttt cttccccgct tgatccgtca cgtagaagac gtcggcgacc tgatccaagc
     5581 gtgtggaaat ccgcgcggca tgaatcgaaa gtcccagctg aaagatggca ttggtaatga
     5641 catgcagcag gccctgccgg tcgtcggcaa atacatcgac gatggtaaag ctgtcggacg
     5701 tttcattgtc gatccgcact tcggtcgcat cgggagcctt gggcaacggc cgcgtcaacc
     5761 gcaatcgcgc tccacggcgg atcacctcat cgacatgctc ccagcctttc aacacagcgg
     5821 cgatgcgatc gccgacggcc tctctgcgat cggccggtgg ttcgcccagg tagtcggggt
     5881 cggtcacctg gaaggtatcg accacaatac cgtcggccct cgtcaggatc tgtgcatcaa
     5941 ggatctgcag tccatttccg gccatgacgc cggtgatttt ggaaaaaatc ccgggggtca
     6001 actcgttgtg ggtgatcacg gaatattcac aggtgcccaa cggacggttg aaatccgtct
     6061 cgacgaccac atcgccctgg tgtaaccggc tcaccgccgc caggtgggcg gcgatgcggg
     6121 ccggaacggt gccatagacg tatcgttcgg ggaattgctt gagctgcgct tcaatccaga
     6181 cacgatcggc cacagggtcg cctgtcgatt tcatgtggcg accgacctct tcgacgactt
     6241 gcgaccagcg cgccggcgct ccctgcacct cgcgatcacc ggagacttcc ggcatcgtcc
     6301 tcgtatacag ctcgatcagc aacgactctt tccattttgt cagcacgcca ggcccgacag
     6361 cagcgatgtc ggcggccgtc aacgccagca gtttcttgag cacctccggg gttccgactt
     6421 cgcgcgcaaa gggaagcaca actttgtcat cgttgggatc acggcgaaag gcagtgtgcg
     6481 ccatcagcaa atggcggtgc acgagaaacg ccaacgtcct cgtttcctgc tcatcgagtc
     6541 caagacgaag cgccgcctcc tcggacaacc gcttgcccac gtcgctgtgg tcctcctcac
     6601 ggcccttgcc cagatcgtgc atcaagaccg cgaggtggag aatgtctttg cgtttgatcg
     6661 ctcgatacac ctctcccaat acgccggtat cctgactcaa cgcttcagcc cggcccacgg
     6721 ccagcagact gtgctcgtcg actgtgtact tatggtattg attgaattgc atcaggcccc
     6781 gtaccgtccc catcgccggc accaacttct cgagcaagtg ggcccgatgc atggcttcca
     6841 gcgtgccggt cgtgcgcggc cccgacagga tccggaggaa cgcgcggctg acttcgggcg
     6901 tgcggtatgc gtccgcggac acctgatcga catggcggtg gatgtcctca attaacggag
     6961 gcacgatggt caggttcctg tcccgggcga gatcgaacag gtgaagcagc agcgccgggc
     7021 tgtccaacac ccggtcacgg tgctcgtccg caaccgaaag caggcttccc cgaacgacaa
     7081 agtagccgtc cactcgcggg gccggaagga agcgcgccat ccgccgccaa aacgggacgc
     7141 ttcggcagcg ctcgacgaat cgcatgcagc gttcgtgcaa tcccatggtg tgctggtaat
     7201 actgctgcat gaactgctcg accgcgagca gatgtggttg atcatggtag ccgaagtgct
     7261 gcgccagcca gacttgctcg tcgaacgtga ggatttcctg cgccgttccg gcatgaacgt
     7321 gaagcagcga ccgaacgcgc cacaagaatt cccgtgcctc ggtcagcgcg agcaaatccg
     7381 ctcgggagag aatgccgcga tccgaaagtt cacgaatggt cggggcctga tagcgcgcca
     7441 taccggccca ttgcagcagg tggagatccc gaagaccgcc tttgcttttt ttgacgttcg
     7501 gctccagcag atacacggtt tcaccgaact tctcgtattc ccggcgtcgt tcctcgagtt
     7561 tctgatccag ataggcatcc gtattcttga acacgacttt gcggaggtac ttgcgatgaa
     7621 agacctggaa cagctctgcg cttcccgcga gaaaccgcgc ttccatcatg gaggttttga
     7681 cggtggcatc cgtttcagcc aactcgatgc tgtcgccaat ggtcctgacg ctgtgaccga
     7741 cctgaaatcc gcaatcccac aatgggtgca ggacggcgcg gaccagggat tccaccgcct
     7801 tgtccgcgcc cggctcgaac aagaacatca gatcgatatc ggattgcgga gccaactctc
     7861 ttctgccgta gccgcccaac gccatcagac agcattggcg catgccgacg gtcgccctct
     7921 cttcgccgcc ctgccgcaag gcatcgcgat aacgtccaag gatcaacccg tcgacgaatt
     7981 ccgtcattga cgccagggtc tcggcaccag agactccggc catgacgcgt tgtgcgatga
     8041 gagaccggtg ctcttcaagc acatggacga ggctgggggc ttccgaaagg gaggacgaag
     8101 aagattcgcg ttccatgata ggtgttacag ggccgtctcc cctgtttctc cggtgcggat
     8161 gcggacggcg gcgctcaaat cacgcacaaa gatcttcccg tctccgatgc tcccggtctt
     8221 ggccgcccgt gaaatcgttt ccaagacgcg tggcacctgt ccatcctgca cggccacctc
     8281 gatcttcacc ttggggacga attcgatcgt gtattcctgg ccgcggtagg tttctttgtg
     8341 ccctttttgg cggccgaatc ctttgacctc cgtcaccgtc atcccctgca caccgatttc
     8401 caacaacgcg tccttcactt catcaagctt gaacggcttg atgatggctt caacgagttt
     8461 catatgcggt ctccatgctc agcattccgt cacgagtagg cacgttcgtt atgttgtgtg
     8521 agatcaagcc ccgtcgattc ctcttcctgg gtcacgcgca acccgatcga tccgtcgaca
     8581 agctttaaga tggcgtaact gcctagaaaa gaaaatgtgc cggtcgctac gacggcgaga
     8641 atctgcatcg ccggatggcc gtgtgccgcg aagaatccga cgcccagcat gccgagcatg
     8701 ccgccaacac catgcgttcc catgacgtcg agcgtgtcat catagccgat cttccccttc
     8761 caaacaattg ccatataaga gcaaaccccc gcggcaatgc cgagcgccac agcggccagc
     8821 ggcccgagca ttccggcgcc ggcagtcgac gtcgccaagc cggcaaccgc gccgctggcg
     8881 actcccaaca cagtcggctt tcctcgatgc ttccattccg ccagcatcca cgcgagggcg
     8941 gcggaaacgg cggtgaagtg cgtcaccgtc acggccccga ccgccgtggc attcgctccc
     9001 cgtgcgctgc cgccgttgaa accgaaccag ccgagccaca atagaccggc gcccaacaaa
     9061 ataaagggca gattatgggg cgccatgtaa tcggtgcgcc agccgcgccg cttgccgact
     9121 accatcgcac acgcgaccgc gctgaacccc gcgctgacat ggacaacggc gccgccggca
     9181 aaatcgagcg cgccgattct cgccagccac ccgtttcccc atagccaatg ggcgatcggc
     9241 gtatagacca ggacggacca gagagcagcg aacaacaaca tcgcaccgaa cttgatccgt
     9301 tcggcaaacg ctccggcgat caaggcgggg gtgaaggccg caaagagcag ctgaaagagc
     9361 atgaacgcct cgagcggaac ggccggcgca tacacaggat ggggcgcagc tccgacgtcc
     9421 atgagcaatg cccaatccaa tccaccaatc acacctcccc ggtccgggcc aaacaccagc
     9481 gtgaagccac agaccagcca gacgacgctg acgacgcaga gaatcgcgaa cgcctggacc
     9541 atcgtgctga tgacgttctt actgcgaacg agaccaccat aataaaaggc cagccccggg
     9601 atgatcatgc ccacgaccag cgccgatgca gccagtatcc aggcgacatc gccgctgtcc
     9661 acgctgcctt ccggttggcc tgcccaggcc tgcgtggcac aaacggtcac ccacaagcct
     9721 gtgatcgcgc ctcgtgacac ccagtgacgc cgttcactca tgccgcctcc ttgctaaaac
     9781 catgatgcaa aacaccttgc ctgctgtacc gcacctgacg agcctacgtg cctcggtatc
     9841 cgttcgtaat cggcatgcgt cgatcttttc ccagtccccg atgggtaatc ttgatgccca
     9901 ccggtgcctg gcgtcgcttg tattcactcc gatccaccag actgacgacc cgcaggacgg
     9961 tctttcgtgc gaatcccatc ccgaccattt catccacgga ccggtcatcc tcgacatagg
    10021 ccttcaaaat cggatcgagc accggatacg gcggcagact gtcttcgtct ttttgatccg
    10081 ctcgtaactc ggcagtcggg ggtctatgca gcgttcgctt cggaatgacc gcgctctgcc
    10141 ggttgcggcg gtcggccaac gcgtacacca tagtcttcgg cacgtctttg atcacggcaa
    10201 acccgccggc catatcgccg tacaaggtgg cgtagccgac gctcatttcg ctcttgttgc
    10261 ccgtcgtcaa cacgagatgt ccgaacttgt tcgataaggc catcaacaaa ttgccgcgaa
    10321 tccgcgcctg cagattctct tcggtcgtat cgaccttggt gccagcgaac acaggcttta
    10381 gcgtctccag atacgacttg aaggtcgggg taatcggcag cgtcctgact tgaatcctca
    10441 ggcggcgcgc caactcctgg gtatcctcgt agctttcgcg agaggtatag ggtgacggca
    10501 tgaacacgcc cagcacattc cggggaccca gcgcgtccac cgccaggacg gcggtcagcg
    10561 ccgaatcgat tcccccgctc agaccaatga cgactcgctt gaacccgttc tttgtcacgt
    10621 aatcccgtac tccgagcgtc aaggctcgat acacttcgtc caacggatcg gccaacggtt
    10681 cgatcgatgg gatcagtcgt gtccgtctcg cgggcggctt tccctttagg gtaacccgat
    10741 cgatggccgt gggcgtcctt cccctgacat ccggtcgtct cccctccgcc gcccgtgccc
    10801 tggcaaccga ctcgacgttc agatcgacga ccaataagtc ctcttcaaag gccttcgctc
    10861 tggcaatggg ctcgccggta tgatccatga caacgctgtt gccgtcgaaa atcagctcgt
    10921 cctgtccgcc gacggtgttc gtatacgtga cgatcacccg gttgtcgcgg gcacgcgtcg
    10981 ccagcatttc ttcgcgtgac cggctcttgc ccccgtggaa gggcgaggca ttgatgttga
    11041 cgatcacctc tgctccggca ttggcctgca gtctcgtcgg cccttcggaa agccagatgt
    11101 cttcgcagac gttcacgcca acgaccgtcc cgtttaccgt gatgagcggg agacggcgtc
    11161 ctggattaaa gtaccggctt tcgtcgaaga cgccataatt gggcagatac catttggcgt
    11221 aggtcagggc caggcggcag tccgtgatga cggctgcggc gttgtacaag tcgtgtcgcc
    11281 cggcaggaag caccgccgcg gctccctctc tcagccctga agcgcgtccc tgtcccacat
    11341 agccgatgac cacgttgcaa tgtcggcacg cacgaacaat ctcgttgagc gcacggcgat
    11401 tgtccgccac gaactgcggc ttcaacagga ggtcttcagg cggatacccg gtgatggcca
    11461 attcgggaaa cgcgaccagg tccggcttgg ccttccttgc ttccaccacc cagttgatga
    11521 tccggcgcgt attgccgtcg agatccccaa cggtgggatt catttgaacc atggcaatgc
    11581 gaaggatgcg catccaacta tccatttcca gtcggcactg ccgcagaatg ttcaatgcgg
    11641 ctgtccggca aagccacaac gaagctcata cgccgaggag gtacataccg agcttcgctt
    11701 gaaccgctcg cttcgatcaa atgcgagcgg ataggcattt tgcctccagt ggacgaaatc
    11761 atacgtcgag ggtccgcgcg agtgagaacg aagccggcgg tcgctttcag cattcttcct
    11821 aaaaaaaaac gccctcggat cccttgcgag gaccgaggac gtcattgtcc gtcatgctct
    11881 taatttggtg tggagactcc attgtctccg aatgacgggc ggaggatagc acccggctcg
    11941 atggagtgtc aaaccgtgga cgacgaatcc acgtgaccac ggacccgctg ctcaatattt
    12001 cctcaaggcc agaggcggaa gttgccacag tagaccccgt acggcacccc acaactccgc
    12061 caatgccaga gtcattgccg gggccgattg tgacatgagt ttgaccacga ggcctgaagc
    12121 ggcgggctga gacaccccgc caaggatggc ttggtccggt tgcttctcca cgatgtcggc
    12181 aagatccgcc tccagggagg ccctcagagt tccggtgatg gcatccccga cgacaaacag
    12241 cgagccgatg taatcccagc gttcagccag gccgacgcca ctccatttgt ttccgggaac
    12301 gatgaggtaa cgctccgcca gtgatgagcc tgaggctgat tcaatgtgaa tccggttgct
    12361 gagcgtggtg aatttccacc gctctccatg ggcaatccgc ccggaagcta tcgcatccca
    12421 caacacaatg gtggctcggg ctgccagctt cacctcgatc gtttgccgga atcgggaacc
    12481 ggcaaaagga attgtatgct ccggtatcca ctcgacaatt cctccgtccc ccactttgag
    12541 agtgacctct tggacggagt cctccgacag agatcgatac acacgattgg ccgaaggggt
    12601 cgagatcagc gcatgcccct tgggaccaac cgacacatcg atggaaagcc ggtcgccacc
    12661 gacaaggcct cccgaaggat tgaccagcag tgtgtacgca ctgcctgagt catcgagata
    12721 cataggagga aagagatgcc agggcgtctg gcagcttgtg cgtccgaaga tcgtgcgatc
    12781 gcctcgccgg acgtattcca gtgccaatgt gccgacccga ccgattgatg ccgtccggcc
    12841 ctccgaatcc gagcgagacg acgcggcacg ggaccctcgg gcgcccttag tgatggtggt
    12901 catgcggatg gatgtgcgac tcgacccagc tcacgactcg atccaggccg tcgccggaca
    12961 tgaggtttgt gaacacaaac ggccggtcgc cgcgcatgcg gcggctgtcg cgatccatca
    13021 cgccgagatc ggcgccgacg tagggtgcga gatcgatctt gttgatcacc agcaggtcgg
    13081 acctggtgat gcccggccct cccttgcggg gaatcttatc gccctctgcc acgtcgatga
    13141 cataaatcgt atggtccacc agctccgggc tgaacgtggc agctagattg tcgccgccgc
    13201 tttccacgaa catgagctcg acctcgggat gtctgcgcaa gagttcgtcc agcgcctctt
    13261 gattgtggga ggcatcttcg cgaatcgccg tatggggaca gccgccggtc tcgactccca
    13321 gaatgcggtc ctgcggcaat gcgcctcttc tggtgagaaa ttcggcatct tctttcgtaa
    13381 aaatatcgtt tgtcacgacc gccagactga atcgatcacg cagcgttcga cagagtgctt
    13441 ccaccagggc agttttgccg gatccgaccg gcccgccgat tccgatcacc ggaatctgct
    13501 ttgagcgggc tacggtgact cggccgcttc cacagtactg atcatcggct cgatgcattg
    13561 aacccctcaa aagatatccg gcctacccgt tccggcagcc acaggcataa gactggagct
    13621 gaccgtttga cgttcaggac cgaaacaacc gtgactccaa ccgggcgtgc cgcatcgcat
    13681 aaatgtcgtg caccggagac cacgagacta agcgtgtctg cggcttcgcc cgacgactga
    13741 gttcggtgat caacggcccc caatcatgga ggagctgttg gccctcctgt tgacctaccg
    13801 gcaacagctt cattgccgcg gacacaaatc cctgcactgt ctggtagaga aacgccgcaa
    13861 cggtgtcttg cctcgaccac ccgcacaccg ccagcgtcat accgatcgcg acagggacgt
    13921 ggccgggtgt ccgcccttcg tcgatggcac gacgaaactc tttgaccacc ctgttctcat
    13981 tagtctgccc ggcagcccct ttcataactt ggcgcccgat ttgtcgactt gccaagcggg
    14041 agtcacgacc cagctttagt tcatccaact gacgatcgac cgccagtgcc agatcaagcc
    14101 tgtctgtcgt ggcagcaaga tggccggccg ccacaccaac cgcctcccgt ttcgcgatac
    14161 cctgcaccag cagatcctcg acatatcctg cgaggtcctt cccgttatgg acggccccgc
    14221 atttgaccgc cgcttccagg ccggaagaaa aggcaaaccc tccagacggg aaaaaactat
    14281 cgacgaagcg caacccttga atgagagaca gcgtgtccat agcgtctgat tccactaagc
    14341 tgagtggcga ttttttatca gcctagaaca agaagtaccg ttgcgccatc ggttgcacaa
    14401 tgatgggctc acaggtcagt cgctccccgt cagccgtcac cacatacgtt tcagggtcga
    14461 cctcgatctg gggcaaggca tcgttcagtt tcaggtcgcg cttgtgaaca gtgcggcacc
    14521 ctttgaccgc cacgatccgt cgctgcaagc cgagctgatg gggaacatcc cgctcc
//
LOCUS       DASSCQ010000213         7267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_302284, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000213 DASSCQ010000000
VERSION     DASSCQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302284"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFH05_13405"
     CDS             <1..585
                     /locus_tag="VFH05_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679267.1"
                     /translation="LTIKDVVESFGHYFWDVISVGFVLWLPIMALDMGTRQNPNGPLI
                     SYAVMLLIFILLNPAPEIIYQVRHDSPLDVFKRAYEFVLENWIEWFLPMALLLLPLLV
                     APDGLRTFFLLSQRVGRGAGLDFSQLLIMPFTILGSWLASVGIDPDLSQYLVLFFTPP
                     LAVSILLFRGHLFALLSRSSHRQRQFASQVKGKD"
     gene            757..2229
                     /locus_tag="VFH05_13410"
     CDS             757..2229
                     /locus_tag="VFH05_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6679268.1"
                     /translation="MRLSIFWRIVLTSLLIIVVMAAVNFYALFQLRQLSALSTTLASQ
                     HYPAIEQAKHLQAVAFNQLNSEKKYLAVRDPTFLQHFDEEVEEFHRGVENLLRQDSSE
                     EGHQFLEQARRYQQDRLALFHAELENAASAPGSVSPEYESRRNEVMDHMTATLDHYVD
                     YHEGVISAGVGRSRESSAQAEAITEQLVLLALVFGVGLAGIASYTILRPLRQLQGHIK
                     QIGQGNFRASLNIRAPSELRDLVDTVNWMGGKLQELDDMKGEFLAHVSHELRTPMASI
                     QEGTHLLLDEIPGPVTQEQRTTLRIMADSSRRLITLISTILDLSKMDAGMMEYRIVPT
                     DLKRITDMSVNKIRLLADAKHVQLVVEAPAQRVWVKADAIRIEQVLDNLLSNALKFSP
                     EGGIVKTQMKSDHKAGVLEVAVSDVGPGIPADELPHIFERFYQGSTKSRHSTPGSGLG
                     LALAKKVVEAHGGRIWIESEVRKGTTVRFILRLTKPGAAT"
     gene            2226..3005
                     /locus_tag="VFH05_13415"
     CDS             2226..3005
                     /locus_tag="VFH05_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679269.1"
                     /translation="MKAMRMASGGLALILMTGGGCSSWSSPPQMSQPYFVVDPSDAKT
                     FHMIGKKQDAVAAKCQEHNSCDHAYFTRALIGLYESRDVAIRYFEKVIAVAPRGQLAA
                     SSRLWLEQIQQHPAPVERSWFASVMSAPAISVSQVTLGQTSDRLVRDLLDREMMIQQL
                     RASKDADAQILDQLQRELAERDRKVDAPTGKKDPGKGGTEGTNVQALQRQLSERDKKI
                     DELSSQLEALKRIDQEMRDKVRPIRPPSTMVPPTSPEPTNP"
     gene            3026..4444
                     /locus_tag="VFH05_13420"
     CDS             3026..4444
                     /locus_tag="VFH05_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6679270.1"
                     /translation="MEQEHILVIDDEEGLLQLVKMRLNAMGFTVTACTSGREAVNAAK
                     TNRFDLAITDLRLRGEDGLDVTEELLRIHPGLPVIILTAHGSIPNAVEAVQRGAFGYL
                     TKPFDDKELKAKIEEGLSPQRMSREIQRLKSLVNELYGMENVVARSPAMQRLLQQVIQ
                     VADSDATMLLFGETGTGKEVFARVTHANSRRSKGPFVALNCAAIPETLFESELFGHVR
                     GAFTSAHGPKKGLFQSANGGTLFLDEIGEMPLSMQVKLLRAVQEREVREVGAEHPSKV
                     DVRIIAATNKDLGEAVKNGSFRNDLYYRISVVPLFIPPLRDRRDDIPILAQYFLTSSA
                     KRANKDVRGFTPAALHRLVTNLWPGNVRELENAIEKAVVMSRQDMITPDLLPSIGTAP
                     DSQLKPLTEAKEEFEKTYLKNVLQLTGGNISRAAQFAGRYRADFYKMLKKYGLHPSTN
                     KGKPGPEIEEIEEEDHLTEAER"
     gene            complement(4551..5861)
                     /locus_tag="VFH05_13425"
     CDS             complement(4551..5861)
                     /locus_tag="VFH05_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679271.1"
                     /translation="MTNHRLRWTLALITAAATGALYAEGVLISGWIEVVLCVPIAMLL
                     FVARSESSLERLLQNGLITITAISLSLVCLDLILRPTFGHKLHYTPTNITSHKMPELP
                     IVGRWDPNLTIDAEGYGDLAAMAGDPAVQERRRIVFRTDAYGFRNIPRSDTTDLLILG
                     DSFPAGGGTSDEEIFARLLETTYGFHTYNLSFPGGPYDQFVNFAIEWRRLNVASHPRM
                     IWTFYTGNDMDDDGGEVWDISALPWKHGFSAWRVKFKSYRNRSPLRQWIEGIRMKMRG
                     WPKYVITRSLPDGHSVLFLDNQERWGKASRHDVEQHPNYTKLLRTMAAMQKLTAERHV
                     DVTGLILPTKGEVYRWLLEARGAQPEDERASGFAEAVLEACRTTGIVCHDTKPYLISE
                     AKRLYQQEGKLLWWRDDTHIGKYGHAAIAAFIAGSVLNTHAMDP"
     gene            6159..6425
                     /locus_tag="VFH05_13430"
     CDS             6159..6425
                     /locus_tag="VFH05_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679272.1"
                     /translation="MESTNAGYALQFDRHTCSVIESMKPGDEKTVLVVPEINSKGEPK
                     AARARRSQNGEEFVVIMPDGVEAKWAYDDLRSTIIPHYKPKPSA"
     gene            complement(7170..7244)
                     /locus_tag="VFH05_13435"
     tRNA            complement(7170..7244)
                     /locus_tag="VFH05_13435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7210..7212),aa:Thr,seq:tgt)
BASE COUNT         1728 a         1986 c         1987 g         1566 t
ORIGIN      
        1 ttgacgatca aagacgtcgt cgagagtttt ggccactact tttgggatgt cattagcgtg
       61 ggcttcgtcc tctggcttcc catcatggcg ctcgatatgg gcaccaggca gaatccgaac
      121 ggtcccctga tttcctatgc cgtgatgctg ttgatattca ttctattgaa tcctgctcct
      181 gaaatcattt atcaggtgcg tcatgattcc cccctggatg tgttcaagcg ggcctatgag
      241 tttgttttag aaaactggat cgaatggttc cttccgatgg cgctgttgct gctccccctg
      301 ctggttgcac ctgacggcct gagaaccttc tttctgttgt cccagagagt aggcaggggc
      361 gcaggtctcg acttctccca actgctcatc atgcctttta cgattctggg gagctggttg
      421 gcttctgtag gaattgatcc tgacctgtcg caatatctcg ttctcttttt cactccgcct
      481 ctcgcggtgt cgatcctgct gtttcgtggc cacctctttg cattactttc tcgatcttcc
      541 catcgccagc gccaatttgc atcgcaggta aaagggaaag actaggacgc tatccttgac
      601 agcctgcccc ggaaactcaa cggcttacag ataagagaac ggggttcatc gcaggtggac
      661 ccatgttcgc tcttgagtga caagtgaatt tagtgtatgg atttacctac acacagagcc
      721 ccctcgtctc tcgatacatc aacgggagct gcactcgtgc gtctgtcaat attctggcgc
      781 attgtcctca catcactgct catcattgtc gtgatggccg ccgtgaactt ctatgcgttg
      841 tttcagcttc gtcagttgtc cgcattgagc acaacgctcg cctcccaaca ttaccctgcc
      901 atcgagcagg ccaagcatct ccaagcggtg gccttcaatc agctcaatag tgagaaaaaa
      961 tacctggctg tccgtgatcc gacatttctt cagcactttg atgaagaggt cgaggaattc
     1021 caccgaggcg ttgagaattt attacgtcag gattcctccg aggaaggcca tcaattcctc
     1081 gagcaggcgc ggcgctatca gcaagaccgc ctcgccctgt ttcatgccga actggaaaat
     1141 gccgcaagcg cgccgggttc cgtgtcgccc gagtatgaga gccgccgcaa tgaagtgatg
     1201 gatcatatga ccgccacgct ggatcattat gtggattatc acgaaggagt catcagtgcc
     1261 ggagtcggcc gctcgcgcga aagttcggcg caagccgaag cgatcaccga gcagctggtc
     1321 ttgctcgcac tcgtcttcgg agtgggcctg gccggaatcg ccagttacac catccttcgt
     1381 ccgctgcgcc agctgcaagg ccacatcaaa caaatcggac agggtaattt ccgggcgtcc
     1441 ctgaacatcc gtgcaccgag cgaactccga gatttggtgg acaccgtcaa ttggatgggg
     1501 ggaaagctcc aagagttgga cgacatgaag ggcgaattcc tggcccacgt ctcacacgag
     1561 ctgcgcactc ccatggcttc catccaggag gggacgcacc tgctcctcga tgaaattcct
     1621 ggcccggtga cgcaggagca acggacgacg ctccgcatca tggccgacag tagccgacgc
     1681 ctgatcacgc tgatctccac catcctggat ctctccaaaa tggacgcagg catgatggaa
     1741 tatcgcatcg tgccgaccga tctgaagcgg atcaccgaca tgtccgtcaa taaaattcgt
     1801 ctcttggccg acgcgaaaca cgtgcaactc gtcgttgaag caccggcgca gcgcgtatgg
     1861 gtgaaggccg acgcgattcg gattgagcag gtcttggata atttgctatc taatgccttg
     1921 aaattcagtc ccgaaggagg cattgtcaaa acgcagatga aatcggacca taaggccggc
     1981 gtccttgagg tcgcagtctc cgacgtcgga cctggaattc ccgcagacga gctgccacat
     2041 attttcgagc gcttttatca gggtagtacg aaatccagac attctacgcc ggggagcggc
     2101 ttgggattgg ccctcgcgaa aaaagtcgtc gaagcacacg gaggcaggat ttggatcgaa
     2161 agcgaagtga ggaaaggcac gactgtacgg tttatcctgc gcctcaccaa acccggtgcg
     2221 gccacatgaa ggccatgagg atggcgagcg gtggactggc gctgattctc atgacgggag
     2281 gaggttgtag ttcgtggtcc tctcccccgc agatgtcaca accgtatttc gtcgtggacc
     2341 catcggatgc caaaacgttc cacatgattg ggaaaaaaca ggatgccgtg gctgcaaagt
     2401 gccaggagca caattcctgc gaccatgcgt attttacccg tgcattgata ggattatatg
     2461 aaagccggga cgtagccatt cgctactttg aaaaggtgat tgctgtggca ccgagaggcc
     2521 aattggctgc atcaagccgg ctgtggctcg agcaaatcca gcaacatcca gcccctgtgg
     2581 aacggtcctg gttcgcctcg gtcatgtcgg caccggccat atcggtcagc caagtcacgc
     2641 tgggacaaac atccgatcgt ctggtgcggg acttgcttga tcgggaaatg atgattcaac
     2701 agcttcgcgc cagcaaggat gccgatgccc agatactgga ccagctccag cgggaactgg
     2761 ccgagcgtga ccggaaagtc gacgccccga cgggcaagaa ggatcccgga aagggaggaa
     2821 ctgagggcac gaacgttcag gcactccaaa ggcagttgtc cgaacgggat aaaaagatcg
     2881 acgagctctc gagccagctg gaagccctta aacggatcga ccaggaaatg cgcgataaag
     2941 ttcgccccat ccgtccgccg tccaccatgg tgccgcccac gtcaccagag ccaaccaatc
     3001 cataacacaa aaggatcagc acctcatgga acaagagcac atcctggtca tcgacgacga
     3061 agaagggctg ttacaactgg tcaagatgcg gttgaacgcg atgggcttca ctgtcaccgc
     3121 ttgcacgtcc ggacgagaag ccgtcaacgc cgcaaaaacc aatcgattcg atttggccat
     3181 cactgacctc cgtctgcgcg gggaagatgg tttggatgtg accgaagaat tgctgcgcat
     3241 ccatcccggg ctgccggtca tcattctgac cgctcacgga agtatcccca atgcggtcga
     3301 agcggtgcag cggggagcct tcggctattt gacaaagccg ttcgacgaca aggagctcaa
     3361 agccaagatc gaggagggcc tgtcgccaca gcggatgagc cgcgagatac aacggctcaa
     3421 atctcttgtc aatgagcttt acgggatgga gaacgtcgtc gcccgcagtc ccgccatgca
     3481 gcgcctgttg caacaggtta ttcaggtggc ggattcagac gccacgatgc tgctcttcgg
     3541 agagacggga acgggcaagg aggtgtttgc ccgcgtcacg cacgccaaca gccgaagaag
     3601 caaaggcccg ttcgtcgcat tgaactgtgc cgccattccg gagacccttt tcgagtccga
     3661 gctgttcggt catgtacgcg gcgccttcac cagcgcgcac ggtcccaaga aaggtctgtt
     3721 ccaaagtgcg aacggaggca cgctgtttct cgatgaaatc ggagagatgc cgctgtctat
     3781 gcaggtcaag ctcctgcgcg ctgtgcagga gcgagaggtg cgcgaagtcg gtgccgaaca
     3841 tccgagcaaa gtcgatgtcc ggatcatcgc ggcgaccaat aaggatctgg gcgaagccgt
     3901 aaaaaatggg agcttccgca acgatctgta ctaccgcatt tcggtcgtgc cgttgttcat
     3961 tccgccgctt cgcgaccgac gtgacgacat tccgatcctt gcccagtact tcctgaccag
     4021 cagtgcaaaa cgggccaata aagacgtacg cgggttcacc ccagccgcgt tacaccgcct
     4081 ggttaccaat ttgtggccgg gaaatgtccg cgaactcgag aatgccatcg aaaaagccgt
     4141 cgtcatgtcc agacaggaca tgatcacacc ggatctgctc ccgtccatcg gcacggctcc
     4201 cgactcccaa ttgaaacctc tcacggaagc gaaggaagaa tttgagaaga cgtatctgaa
     4261 gaacgttcta caattgacag gcggcaatat ttcacgcgct gcccaattcg ccgggcgcta
     4321 tcgagcggac ttctataaaa tgctcaaaaa gtacgggttg catccttcga ccaacaaggg
     4381 caagcccgga cctgaaattg aagagatcga agaagaagat catctcacgg aggcagagcg
     4441 gtaggtcgcg cctcaccaca tcgtccgccc aggcgactac ccagtgttct cacccaagac
     4501 ggtgtccggt tcctgccgga ccttcaatcc agacgtcgtg cacgcacaat tcagggatcc
     4561 attgcatgcg tgttcagcac gctgccagca ataaaggccg cgatggctgc atgaccatat
     4621 ttcccaatat gcgtatcgtc tcgccaccac aagagcttgc cttcttgttg gtagagtcgc
     4681 tttgcctctg agatgagata cggtttcgta tcgtggcaca cgattcccgt ggttctgcac
     4741 gcctcaagga ctgcctcggc aaaaccggag gcgcgttcgt cctcaggttg cgcgccacgg
     4801 gcctccaaca gccagcgata gacctcaccc ttcgtgggaa gaatcagccc ggtgacgtcg
     4861 acgtgacgtt cagccgtgag cttttgcatt gcggccatcg tccgtaacag cttggtgtag
     4921 ttgggatgtt gttcgacgtc atgacgggat gctttccccc atctttcttg attatcgagg
     4981 aacaggacag aatgaccatc cggtagagac cgagtaatca catacttggg ccatccacgc
     5041 atcttcatgc gtataccttc gatccattgc ctgagtggcg agcgatttct gtaactttta
     5101 aacttcaccc gccaagcaga gaagccatgc ttccacggta gcgctgagat gtcccagacc
     5161 tcacctccgt catcgtccat atcgttcccg gtgtaaaaag tccagatcat ccgagggtgg
     5221 gaagcgacat tcaatcgtct ccactcgatg gcaaaattaa cgaactgatc atacggtcca
     5281 ccagggaagg aaagattata cgtgtggaag ccatacgttg tttccaacaa gcgagcgaag
     5341 atttcctcgt cgcttgttcc gcccccagcc ggaaaagagt cgcccagtat caacagatcc
     5401 gtcgtatccg agcgggggat gttccggaag ccgtatgcat ctgtccgaaa cacgattcgg
     5461 cgacgttctt ggacagccgg atcaccggcc atggcggcta aatcgccgta gccttcggca
     5521 tctatcgtga gattggggtc ccaccgaccg acgatgggca gttcaggcat tttatgagag
     5581 gtgatattgg tgggagtata atgcaattta tgaccaaagg tcggtcgcag gatgaggtcc
     5641 aaacacacga gcgacaggct gatggccgtg atggttatca gcccattttg caagagacgc
     5701 tcaagggatg actctgaccg ggctacaaac aataacatgg cgataggaac acacagcaca
     5761 acctcgatcc accctgaaat gagaaccccc tcggcataaa gagcccccgt cgctgcagcg
     5821 gtaatcaagg ccaaggtcca acgtagtcgg tggttagtca caccatgctc cttacgctcg
     5881 atgttggtgg aggttgtgtt tgatcgggga gatgcaggtt acaggacaag gccggctttg
     5941 cgcaacagca gcgtgcatgt tgcgggacgt cgttcttagc caatcgtagg catgtgatcg
     6001 cttacaaagg tgatcgcgat agcagctcac cgttcgcgaa ggcagtatgt aagaccggcg
     6061 cgcatgaggt cagttttgat tgcttgacct tttctcctcc attggtaaga ctaagagagt
     6121 ctgaactcat cattcgaatc tggctgggag gtactggaat ggaatcgaca aatgccggct
     6181 atgctctcca atttgacagg cacacatgtt cggtaattga gagcatgaaa cctggtgacg
     6241 aaaagacggt tctggttgtt ccggaaatca attcaaaggg cgaacctaaa gcagcgaggg
     6301 caagaaggag ccagaacggg gaagagttcg ttgtcatcat gccagatggc gtggaggcca
     6361 aatgggctta cgatgacctg aggagcacga tcatcccgca ctataaacct aaaccatctg
     6421 cctaaagaaa acgcgttgca tataggcagg cttgcaagtg tctgcctata atcttgactg
     6481 gtctacagcc ggctagcgtt cttcgcagca gcacctcagt cgatcgaccc gtccgactgc
     6541 tctccactga ccgcttagtg tgaggagttc taaccagaat tctgcagtta ctcttgagag
     6601 aacttgacgg cgttgggctt gataggatga aaagaggtag gtgatgtcga gagtagcatc
     6661 ctcagtggac cgtctcctac gaggccatag cgagaacaga gggaacttca agtggaccag
     6721 cccacatggc gtccacactt tgaagattga cctccgtgct agaagtcttt ggatagtggg
     6781 aagcaggaat tagcgccaac caacgacgag tctgccgatc aaatacgatc tgcactatcg
     6841 tcggtaggtt actcccttta ctggggctca acatccagta ccacccaggg gtgttgggat
     6901 tctgctctgt ccatgtcagc atccggtccc tccgtgtccc atcgtaatca tcaacgttgt
     6961 tcaccccatc gtaggcacac cggaaaagac gacgctattc cctaaaggag gtaggtaaag
     7021 gtggacgaat cgctaggggg gggaggccag gatttgctat aggaaatcct gtatgcagtc
     7081 gctcaaaaca ggtacaacat cgtgaatgaa ctgagcccga agcggtagga caactgatga
     7141 aggtttggtt ccggcatgac cggaaagcct ggagctggcg agaggaattg aacctccaac
     7201 ctgtggttta caaaaccact gctctgccat tgagctacgc cagccagcta gattgctgag
     7261 gaaggac
//
LOCUS       DASSCQ010000214         6425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1773183, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000214 DASSCQ010000000
VERSION     DASSCQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773183"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1300)
                     /locus_tag="VFH05_13440"
     CDS             complement(131..1300)
                     /locus_tag="VFH05_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET6679273.1"
                     /translation="MPTRAFLVILALLLWSIGCQPEEPSGKAQRPPLQADMQPVEVQV
                     MRPIRKDLAYTVKLPANIAPLYQTTLYAKVSGYLKWIGPDKGDPVKKGQVVSIIDAPE
                     IEEQYLQAAADYKIKKITFERLKNVWKESPDVIAKQDVDVAEAAYEGARTLMQQRAVL
                     RDYTKVRAPYDGTVTARFADPGALIQVATSSATSAIPLFTIMDLSTVRIYANVSQDDS
                     PWVTPGKTHATVTVPELPGRSFSGTITRSTLALDPSTRSLLVEIDLPNPHHLLRPGTF
                     AEMAIALREIPNALVVPPQAVSSGGKGPSLFIVEQGKAKVVPVQTGITDGKWVEITSG
                     LKGDEEVVAVGKRQLLDGSPVRVSPFQLPDATFSQQRYERRSPGVQEAETPAVPR"
     gene            complement(1305..4571)
                     /locus_tag="VFH05_13445"
     CDS             complement(1305..4571)
                     /locus_tag="VFH05_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET6679274.1"
                     /translation="MLTRSALKNPYAVFALCMIALVLGAVSYKKMRVDIFPEIKIPTI
                     LVTTFYRGMSPGEMEGAITLRMEQRFVEASYVEHIESQSLAGMSYIKVFFQPEYSIDA
                     AQSELTSLAYSIIRLLPPGVYPPSVYKFGVASLPVGFLSISSESLGPKDIRDLAYFTV
                     RNQIANIPGISFGPPLGGKVRQITVFMDQQRMLARGISPSDVVKAINAQSAIIPAGDI
                     KIGDLDYYVYSNSLVGVVEKINDLPIKVVNGTPILVRDIGTAADSAAIQTSIVRVNGR
                     EATYLPITRQEGANTLEVTDGVRAKLPKLTEIPAGTTVKFIYDQSLYIRQAIANLQKE
                     GLLGAGLAGLMIFVFLRSMKAALVVGLAIPLSLTAALVALFLSGQTVNIMTLGGLALV
                     IGTLLDNNIVVQENLHRHLEMGKDGRSAAEDSATELTLPIFVATLCILIVYLPIIFFA
                     GIVKYLFVPLAMTVAFAMLADYAVSMTVTPVVLARLYSRGHGNESGDDASAAHGWFRF
                     VLAAYEPVLRTGLRLKWLVLILALTALLVVSITLLPRLHTEFFPKVDAGNFTMLVTAP
                     EGSRIEKTTAIVADIERLVQETVPKQELEEVVSNTGLYFGDAARFAPNTGNHTAFVLV
                     NLVSGHTGRTDSYIDALREKTRKSLPGVEITFQTGGIISDVLNFGLRAPIDIQIKGPS
                     LDLIRPVAEHIRQEVAQVANTTDVRIKQGKSYPELHVNVDRTKAAYYGLTQDKVIVDV
                     ITGISSNLALSPNYWLDPKTANGYFLLAQYPEQSLTKTEDLLNIPVIGARTALNPTSS
                     LTTAGSSGSTIALQNTPFAGRNLDLSAGFFASDERRGPPVLLRDVASLEIKTGPDTVD
                     HYDLSRLLDVLVTPVGNDLGRVAADIEAVLQRIGLPKDVTIELKGEVANMRAAFNNFS
                     LALPLAVLLIYLVMVGLFRSMVDPLIILVAVPLGWIGTIIMLHLTDTSINVESMIGTL
                     MMMGIVVSNSILLVDFANRMVRAGASADRAVLEAGRRRIRPIVMTALATILGLLPLAL
                     GFGEGNETMVPLARAVVGGLAVSTVMTLLVVPVMHAFVLSRRIHIDPSLTPTASSEEI
                     "
     gene            complement(4565..4996)
                     /locus_tag="VFH05_13450"
     CDS             complement(4565..4996)
                     /locus_tag="VFH05_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679275.1"
                     /translation="MPRHASVLLIDDSPGERELFREALMRTDLDVVLYSEQDAEAALH
                     FLKHRPELPSLILLDWHLRHERGDGFLRRLRSERRFAAIPVVAFTTSDEVADLSAAYA
                     NAVNGYVVKPDTFEDLVQCVEDICRYWLTRNRTPYPVETPC"
     gene            complement(5290..6177)
                     /locus_tag="VFH05_13455"
     CDS             complement(5290..6177)
                     /locus_tag="VFH05_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET6679276.1"
                     /translation="MTHRLYYAVMLLVVFALQTTGCATNPYTKRWQLLMVPQSYEANL
                     GAQAYQDVLSSPKVKISDNPSEVTPVQRVASRIIEAAKKSKYADSAKDFKWEVAVIKD
                     DGTKNAFALPGGKIAVYTGIFPVADNESGLAAIMGHEVVHALARHGSERMSQGVLAQI
                     GLVGASIAMGTQGFSPVTSQAAMTALGVGTQVGVLLPFSRAHESEADYIGLLLAADAG
                     YDPQEAVRVWQRMQQSSDGKQPQEFLSTHPGHETRIKRLTEKMPEALALYNQAQKAPV
                     AQLPPVGGPATAAPLAQQQ"
BASE COUNT         1345 a         1766 c         1978 g         1336 t
ORIGIN      
        1 ggtcggaggt gcgtcctggc ggttgtcctc ccgcgaccga agggatcagc agcgtgaaca
       61 gcagtacaaa aagcgatttt cgtcgaagga tgtcagacat tttggtcctc ctccgtgcaa
      121 catgtgtagg ttagcgtggc actgccggtg tctctgcctc ctggactccg ggtgaacgtc
      181 gctcgtatct ttgctgagag aatgttgcgt cgggaagctg gaagggggac acgcgcacgg
      241 ggcttccatc caacagctga cgcttcccaa ccgcgacgac ctcttcgtcg cccttgagtc
      301 cacttgtaat ctcgacccac ttgccgtcgg taattcctgt ttggaccggc accaccttgg
      361 ctttgccttg ctcgacaatg aagagcgagg gacccttgcc gccgctgctg acggcctgtg
      421 gcggcacgac gagcgcattg gggatctctc gcagggcgat cgccatctcg gcgaaggtcc
      481 ccggccggag gagatgatgg gggttcggca ggtcgatttc gaccaagaga ctgcgggtcg
      541 aaggatccag cgccaaggtc gatcgagtga tggtgccgct gaacgaacgg ccgggaagtt
      601 caggtaccgt cacggtggcg tgggtcttgc caggcgtcac ccagggggaa tcgtcctgag
      661 acacgttggc atagatgcga acggtgctca gatccataat cgtaaacagc gggatggcgc
      721 tcgtggccga cgacgtggca acttgaatca aggcccctgg atcggcaaaa cgggcagtga
      781 cggtgccgtc gtagggggcc cgcaccttcg tgtagtctcg caagaccgcc cgctgttgca
      841 tgagcgtcct ggcgccttca tacgccgctt cagccacatc gacatcctgt ttggcaatga
      901 catccgggga ctccttccag acattcttca atcgctcgaa cgtgatcttc ttgatcttgt
      961 aatctgcggc agcctgaaga tattgctcct cgatttcggg cgcgtcgatt atcgacacga
     1021 cttggccttt cttgacaggg tcacctttat ccggcccgat ccatttcaag tagcccgaca
     1081 ctttcgcata cagggtggtc tgatacagcg gagcgatgtt tgccgggagc ttcaccgtgt
     1141 aggcgagatc cttccggatc ggcctcatga cttgtacctc gaccggttgc atatcggcct
     1201 gaagaggcgg ccgctgggcc ttgcctgatg gttcttctgg ctggcatccg atggaccaga
     1261 gcagcagagc cagaatgacc aggaaggcgc gtgtcggcat aaaactagat ctcctccgat
     1321 gaagcggtgg gagtcagcga agggtcgatg tggatgcgcc gcgacagcac gaacgcatgc
     1381 atgacgggga caacgaggag cgtcataacg gtcgagacgg caaggccgcc gaccaccgca
     1441 cgggccaatg gaaccattgt ctcattgcct tccccgaatc caagcgccag gggaagcagg
     1501 ccgaggatcg tcgcgagggc agtcatcacg atcgggcgaa tgcgccgccg tccggcctcc
     1561 aacaccgccc ggtcggcgga cgcccccgct cgcaccatcc ggttcgcaaa atctacaagg
     1621 agaatgctat tcgagacgac gatgcccatc atcatgagcg tgccgatcat cgattcgaca
     1681 ttgattgacg tatcggtcag atgcagcatg atgattgtgc cgatccaacc taaagggacc
     1741 gcgacgagaa tgatgagcgg atcgaccatt gagcgaaaca ggccgaccat caccaggtag
     1801 atcaacagga ccgcaagagg cagtgcgagc gagaagttgt taaaggcggc tcgcatattg
     1861 gccacctccc ccttcagttc gatcgtcacg tccttcggaa gcccgatcct ctgcagcacc
     1921 gcctcgatat ctgcggcgac acgcccgagg tcattgccga ctggcgtcac caacacgtcg
     1981 agcaaacgcg acaaatcata gtggtccaca gtgtccggac ctgtcttgat ctccagcgag
     2041 gccacgtcgc ggaggagcac cggtggaccc cggcgttcgt ctgaggcaaa gaaaccagca
     2101 gacaagtcga gattgcgtcc ggcgaacggc gtattttgaa gcgcgatagt cgagccgctg
     2161 gatccggccg tggtgagcga tgaagtcgga ttgagggctg tgcgggcgcc gataaccgga
     2221 atattgagaa gatcttcggt cttggtcagt gactgctccg ggtactgggc taagagaaag
     2281 tagccgttgg ctgtcttggg atcgagccag tagttggggc tcagggccaa gttcgagctg
     2341 atgccggtga tcacatctac gatgaccttg tcctgcgtca gtccgtaata ggcggctttg
     2401 gtccgatcca cgttcacgtg aagttccgga tagctcttgc cctgcttgat gcgcacgtcc
     2461 gtcgtgttgg cgacttgagc gacctcctga cgaatgtgct ctgcaaccgg acgaatgaga
     2521 tccaaggacg gtcccttgat ctgaatgtcg attggggcac gcaggccgaa attcaacacg
     2581 tcgctgatga tgccgcctgt ttgaaaggtg atttcgactc ccggaagtga cttgcgggtt
     2641 ttttctcgca gcgcgtcaat gtacgaatcg gtccggcctg tatgcccgga caccagattg
     2701 acgagcacga aggccgtgtg atttcccgtg ttgggtgcaa aacgggccgc atcgccaaaa
     2761 tagaggccgg tattggagac gacttcttcc agttcctgct tgggaaccgt ctcttgcacg
     2821 agtcgctcaa tgtcggccac gatggcggtt gtcttttcaa ttcgagagcc ctcgggggcg
     2881 gtgacgagca tcgtaaagtt tcctgcgtcg accttcggga aaaattccgt atggaggcgg
     2941 ggcagcagcg tgatgctcac gaccagcagc gcagtgagcg ccaagatcag cacgagccac
     3001 ttcagccgga ggcccgtgcg caggaccggt tcgtaggccg ccaagacgaa gcggaaccag
     3061 ccgtgggcgg ccgatgcatc gtcccccgac tcgttcccgt gcccgcggga atagagtcgg
     3121 gccaacacga cgggggtcac cgtcatcgat acggcgtagt ctgcgagcat ggcaaacgcc
     3181 accgtcatgg ccagggggac gaagagatat ttgacgattc ctgcaaagaa gataatcggc
     3241 aggtagacga tcagaataca gagcgtggcg acgaaaatag gaagcgtcaa ctcggtcgcg
     3301 ctgtcctcgg ccgctgatcg gccatccttc cccatttcca ggtgacgatg gaggttttcc
     3361 tgaaccacga tattgttgtc gagaagagtg ccgatgacga gggcgagacc tccgagcgtc
     3421 atgatgttga cagtctggcc gctgaggaag agcgcgacca gtgcagcggt cagcgaaagg
     3481 ggaatggcga gaccgaccac cagtgccgct ttcatgctgc gcaggaatac aaagatcatc
     3541 aagccggcga gcccggcacc caacaatcct tccttctgaa ggttggcgat ggcctgccgg
     3601 atgtacaacg attgatcata aataaatttc acggtggtgc cggcgggaat ctccgtcagc
     3661 ttgggcagct tcgcccgaac cccgtccgtc acttccaggg tattcgcccc ttcttgtctc
     3721 gtgatcggca gatacgtcgc ttcacggcca ttgacgcgga caatggaggt ttgaatagcg
     3781 gcgctgtcgg ccgcggtccc gatgtcgcga acgagaatcg gtgtgccgtt cacaactttg
     3841 atcgggaggt cgttaatttt ctcgaccacc cccacgaggc tgttcgaata cacgtagtaa
     3901 tccaggtctc cgattttgat gtctccggca ggaatgattg cgctttgcgc gttaatggcc
     3961 ttcaccacgt ccgacggcga gatgccccgg gccagcatcc gttgctgatc catgaacacg
     4021 gtgatctgcc tgactttgcc gcccaacgga ggaccgaagg aaatgccggg aatgttggcg
     4081 atctgattgc gcacggtaaa ataggcgagg tcgcggatat ctttcggtcc gagactctcg
     4141 ctgctgatgg aaagaaagcc gaccgggagg cttgcgacgc cgaacttata gaccgagggc
     4201 ggatagacgc cgggcggcag cagacgaatg atgctgtagg ccaaactggt cagttctgat
     4261 tgcgcggcat cgatcgaata ctccggctga aaaaagactt tgatataact catgccggcg
     4321 agtgactggg actcaatatg ctccacgtaa ctggcttcga cgaaccgttg ttccatgcgg
     4381 agggtgatgg cgccttccat ctcgccggga ctcatgccgc ggtagaaggt cgtgacaagg
     4441 atggttggta tcttgatttc cgggaaaatg tcgactcgca tttttttgta agagacggcc
     4501 cccagcacga gggcaatcat gcacagggca aatacggcat aagggttttt caaggcggat
     4561 cgtgtcagca tggcgtttcc accgggtagg gtgttcgatt tctggtcaac caataccggc
     4621 agatatcttc cacgcactgc accaggtctt cgaacgtgtc cggcttcacc acatagccgt
     4681 tgacggcgtt ggcgtacgcg gccgaaaggt cggcgacctc gtcggaagtc gtaaacgcga
     4741 cgaccggaat agctgcgaac cgccgctcgg agcgcagacg cctaaggaag ccatcgcccc
     4801 gctcatgtcg gaggtgccaa tcgagcagaa ttaacgaggg cagctcaggc cggtgtttga
     4861 gaaaatgcag cgctgcttcg gcatcctgtt cggagtacag gacgacatcg aggtcggtgc
     4921 gcatgagcgc ctcgcgaaac agttcgcgtt cgccggggct gtcatcgatc agtaagacgg
     4981 aggcgtggcg cggcatcgag aagcacttca gcagagatcg tgccgtgtgg gggatgaaaa
     5041 aatacgcgat gacttgttct aagagatcaa cgatttacag gcttatctcg taagcctgcg
     5101 cgcgaggagc ctgcggacgg gtctggagaa cttccatagc accatggagg ttttccctat
     5161 cgagaggagg gggaacctca ggacaggtga tcgttgacgt gattgcgctc actcgcagtt
     5221 ttggtcaaag cgctgtaggg gaccaacggc gaggaatgat tatctcgatg aagcggggcc
     5281 ttttctttct tactgctgtt gggccaatgg ggccgcagtc gctggaccgc cgaccggtgg
     5341 gagctgcgca accggcgctt tttgcgcttg gttgtagagc gccagagcct cgggcatctt
     5401 ttcggtgagc cgtttgatcc tggtctcgtg tccggggtgg gtcgagagaa attcttgtgg
     5461 ttgtttcccg tcagaggatt gctgcattct ttgccacact cgcaccgctt cttgcgggtc
     5521 gtagccggcg tctgccgcca acagcaaccc aatatagtcc gcttcagact catgggcacg
     5581 actgaagggc aagagcactc ccacctgtgt acccacgcct aatgcggtca tggcagcctg
     5641 actcgtcacc ggactgaacc cttgagttcc catggcgata gaggctccca ccagaccgat
     5701 ctgtgcgagg acgccctggc tcatgcgttc cgatccatga cgggcgagcg cgtggacgac
     5761 ttcgtggccc atgatcgcgg ccagcccgga ctcattatcc gcgacgggga agatcccggt
     5821 atacaccgcg attttacctc ccggcagggc aaaggcgttc ttggtgccgt cgtctttaat
     5881 gaccgcgacc tcccatttga agtcttttgc cgagtcggca tacttggatt ttttcgcggc
     5941 ctcgatgata cgtgaagcca ctcgttggac tggtgttact tcggagggat tgtcggagat
     6001 tttgaccttg gggctactca ggacgtcctg atacgcttgg gcgcctagat tggcttcata
     6061 ggactgggga accatgagaa gctgccagcg tttggtatag ggattggtgg cgcagccggt
     6121 cgtctgcagc gcaaaaacga caaggagcat caccgcgtaa tatagccgat gcgtcatgtc
     6181 agccctcctg gtagaggata cagagatatt ctaagaggct tggcggacgg gcagtgatta
     6241 ggacatcccc tagggaaagc aatctcgcgg cccggttcag ctgctagatc cttctgtgcc
     6301 accgggatac ccgcggcgga gggagcccgt gaccgagcga ttaggatgat gatgagttcg
     6361 actgaacgac gcggaagcgc acttcgtccg cttgagtata ggcagatgtg cgttcaccgg
     6421 caaag
//
LOCUS       DASSCQ010000215         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1163263, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000215 DASSCQ010000000
VERSION     DASSCQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163263"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1085)
                     /gene="cobT"
                     /locus_tag="VFH05_13460"
     CDS             complement(30..1085)
                     /gene="cobT"
                     /locus_tag="VFH05_13460"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET6679277.1"
                     /translation="MSLTQVLKQICDVDREVAARTQRRLDRLTKPQGSLGRLEQLAVQ
                     YCAITSEGKPAVPRGRVYTFAADHGVVDEGVSAYPRDVTAQMVLNFLRGGAGVNVLAR
                     HAGVEVRVVDIGVAHDFGSVAGLINRKLLMGTRNMVQGEAMDRRTVEEALEIGIELAS
                     EAVKEGVTLIGTGEMGIGNTTASAAITAVMTGASVEQVTGLGTGIDELGRTRKISVIE
                     RALARHEPNADDPIDVLAKIGGLEIAGMAGLMLGAAAARIPVVLDGFIAGAAALIAGG
                     LQPRSVQYMIASHRSVERGHRLVLETLGLEPLFDLDLRLGEGTGACLGMSLVQAAVKV
                     LTEMATFSEAGVSERNQ"
     gene            complement(1095..1640)
                     /locus_tag="VFH05_13465"
     CDS             complement(1095..1640)
                     /locus_tag="VFH05_13465"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET6679278.1"
                     /translation="MAPRRTGSRLVFVLGGASSGKSEVALQSACKGIAKNAPRAFVAT
                     GEGLDEEMAMKIARHRQSRASDWSTAEVPLELTAWVKEHGQAYRTIVLDCLTMWLSNR
                     WRQGSKEQEILQDGRTFLAALRGLEARVVIVSNELGMGLVPGEADSRRFREVAGALNR
                     LVAKEADEAYFVVSGLPLRLK"
     gene            complement(1627..3138)
                     /locus_tag="VFH05_13470"
     CDS             complement(1627..3138)
                     /locus_tag="VFH05_13470"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HET6679279.1"
                     /translation="MAARTLMIQGTASHVGKSVMTAALCRIFVRRGLRVAPFKAQNMS
                     NNSFVTPDGKEIGRAQAVQAAACRLVPRTDFNPILIKPESHSAAQLVVEGEVAGSLCA
                     KDFGAVRRQYWTTVQEAFARLSHTFDVVVLEGAGSPAEINLRHCDVVNMQMARHAAAP
                     VVLVGDIDRGGVFASLVGTWALLDEADRRHAKAFAINKLRGDASLLETGLVRVTDETG
                     MRCAGVVPHWGDLEVPEEDSLGWESWHDRTSKRQDRLVIGIVDVPGISNFTDFDPLAM
                     EPDVELVRLSEATNRPLDAVLFPGTKQTVQALGFVRQRGFDRLARRVLDQGGTVGGIC
                     GGYQLLGQTIRDPQHLESDEDHIDGLGMLPVHTTFAFPKIVEQVSARHAESGCTVGGY
                     QVRMGRTVVSPEARPFLAFQDGRIDTDTHDGICAHDGRLFGTSLHGLFDQSPFRRWWL
                     NRLRTSKEWPGLPETQVLSLDARLDRLADFVEQHLSMSIIDRLLEEGVSYGTS"
     gene            complement(3151..3744)
                     /locus_tag="VFH05_13475"
     CDS             complement(3151..3744)
                     /locus_tag="VFH05_13475"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET6679280.1"
                     /translation="MRITKVYTRTGDGGSTRLAGGQQVSKDCLRVEAYGTVDELSASI
                     GLARAMNEEATPAIAAAKRLEQDLRWVQNKLFDVGSLLATAPGQTFKNMPTIKAQDTV
                     KLERMIDRCQKDLAPLKEFILPGGGKVSATLHQARTICRRAERICVGLAKAEPLDAEI
                     IKFLNRLSDALFVLARWVAKTQGEPEFLWERNTVEGD"
     gene            complement(4022..4183)
                     /locus_tag="VFH05_13480"
     CDS             complement(4022..4183)
                     /locus_tag="VFH05_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679281.1"
                     /translation="MWNASFSYDVNKTVQIYGRADNIFNRKYEEVLFFGTPIRSVFGG
                     VRVNFDMPI"
BASE COUNT          900 a         1400 c         1119 g          883 t
ORIGIN      
        1 ggcaaaaatg aacggcctga gactggtcgt cactggttgc gctccgagac acccgcttcg
       61 ctgaacgtcg ccatctccgt gagtactttg acggctgcct gaacgagact cattccaaga
      121 caggctccgg tgccttctcc gagtcgaaga tccagatcga atagaggctc taaacccagt
      181 gtctcaagca ccagccgatg ccctctctcg acggaccggt gagaagcgat catatattgg
      241 acgcttcggg gctggagacc accagcgatc aatgcggctg ctccggcgat gaacccgtcc
      301 aacacgaccg gtatgcgcgc ggcggcggca ccgagcatga gaccggccat gcccgcaatt
      361 tccaagccgc ctatttttgc caggacatcg atcggatcgt ccgcattggg ctcatgacgg
      421 gccagcgccc gctcaatcac ggagattttt cgtgtccggc caagttcgtc gatcccggtt
      481 cccaggcccg tgacctgctc aaccgacgcc cccgtcatga ccgctgtgat cgccgcactc
      541 gccgtcgtgt tcccgatgcc catctcaccg gtaccgatca gcgtcacacc ttctttcacc
      601 gcctcggacg cgagttcaat cccgatctcc agggcctctt cgacagttcg ccgatccatg
      661 gcctccccct gaaccatatt tcgtgtgccc atgagcaatt tccggttgat caggccggca
      721 acggagccaa agtcatgcgc gactccgatg tccacgacgc gcacctcgac cccggcatgt
      781 cgggccaaca cattgacgcc ggcgcctcct cgcagaaaat tcagcaccat ttgtgccgtc
      841 acatctcgag gataggcact caccccttcg tcgaccaccc catgatcggc ggcaaaggta
      901 tacaccctcc cgcgtgggac agccggcttg ccctctgatg tgatcgcaca gtattggaca
      961 gcgagttgtt cgagccgacc caaactgccc tgaggtttcg tcaagcgatc gagccggcgc
     1021 tgcgtccttg cagccacttc cctgtccacg tcgcagatct gcttcaatac ctgggtcagg
     1081 ctcatagtga tcctttattt caatcgaaga ggaagtccgc ttaccacaaa ataagcctca
     1141 tccgcctctt tggccacgag acgattcaag gctccagcca cctcccggaa tcgccgagag
     1201 tctgcctcac caggaaccaa ccccataccc aattcattcg agacgatcac gacacgcgct
     1261 tcgaggcctc gcagcgccgc gagaaacgtt cgtccgtctt ggagaatctc ctgttccttg
     1321 cttccttgac gccaccggtt cgaaagccac atggtcagac aatcaagcac aatggtccga
     1381 tacgcttgtc cgtgttcttt gacccatgcg gtcaactcca gtgggacttc agccgtgctc
     1441 cagtccgacg ccctggattg ccgatggcga gcgattttca tcgccatctc ttcatccaac
     1501 ccctcacctg tcgccacaaa ggcacgagga gcgtttttgg cgattccctt gcacgccgat
     1561 tgcaaagcaa cctcgctctt tccggaggag gcgcctccga ggacgaacac cagacgcgat
     1621 cctgttctac gaggtgccat acgagacgcc ctcctccagg agccgatcga tgatagacat
     1681 ggaaagatgt tgttcgacaa aatcggcgag ccggtccagt cgtgcatcga gggacaacac
     1741 ttgtgtctcc gggaggcccg gccattcctt cgatgtccgc aatcgattca accaccagcg
     1801 acgaaatggc gactgatcga acagaccatg cagggacgtg ccaaagagcc ggccgtcatg
     1861 agcgcagatc ccgtcgtggg tgtctgtgtc aatgcggcca tcctgaaatg ccaaaaacgg
     1921 ccgggcctca ggggaaacga cggttcgtcc catcctgacc tgatagcccc ccacggtaca
     1981 gccgctctcg gcatgtcttg ccgagacctg ctcgacgatt ttcggaaacg caaacgtcgt
     2041 atggaccgga agcatcccaa ggccgtcgat gtgatcctca tctgattcaa gatgctgggg
     2101 atcgcgaatg gtttggccga gcagttgata gccgccgcag atcccgccga cggtccctcc
     2161 ctgatccagg acgcgacggg caagacgatc gaaccctcgc tgacggacga agcccagggc
     2221 ctgaaccgtc tgtttcgtcc ctggaaaaag cacggcgtcc aacggacggt ttgtcgcctc
     2281 gctcaagcga accagttcca catccggttc catggccaac gggtcgaagt ctgtgaaatt
     2341 ggaaatgcct ggcacatcca cgattccgat cacgagacgg tcttgccgct ttgacgtccg
     2401 gtcatgccag ctctcccatc ccagtgaatc ctcctcggga acttccagat ctccccaatg
     2461 agggacgacc cctgcgcacc gcattcccgt ttcatctgtg acgcggacga ggccggtctc
     2521 gagaagactc gcgtctcctc gcaatttatt gatggcgaag gcctttgcat ggcgacgatc
     2581 ggcctcatcc aacagagccc aggtccccac aagcgaggca aataccccgc ctcggtcgat
     2641 gtcccctacc agtacaacgg gtgcggcggc atggcgggcc atctgcatat tgacgacgtc
     2701 gcagtgtctc agattgattt cggcgggact accggcgcct tcgagcacca ccacgtcaaa
     2761 tgtgtgcgac agccgggcaa aggcctcctg caccgttgtc caatattgac ggcgcaccgc
     2821 accaaagtcc ttcgcgcaca gcgatccagc cacctcgcct tcgactacaa gctgtgcggc
     2881 cgaatgtgat tcaggcttga tgaggatggg attgaaatcc gttcgcggaa ccagacggca
     2941 agcagccgct tgaacggcct gtgcccggcc aatttccttc ccatccggtg tgacgaagga
     3001 attgttcgac atgttctgcg ctttgaatgg cgccacacgc agcccacgac gaacgaagat
     3061 ccggcacagg gcggccgtca tcacggactt cccgacatgc gaagcagtcc cctgaatcat
     3121 gagcgtgcgc gcggccatca ccgatcctga ttagtcgcct tcgaccgtgt tgcgttccca
     3181 cagaaattcc ggctcgcctt gtgtcttggc cacccatcga gccagcacga agagggcatc
     3241 gctcaatcgg ttcagaaatt tgatgatctc ggcatcgagc ggttccgctt tggccaaacc
     3301 gacacaaata cgctccgcac gccgacagat cgtgcgagct tgatgcaatg tggccgacac
     3361 ctttcccccg cccggaagga tgaactcctt gagcggagcc aagtcttttt ggcatcggtc
     3421 gatcatgcgc tcgagtttga cggtatcctg tgctttgatc gtcggcatgt ttttgaaggt
     3481 ctgtcccgga gccgtggcaa gcaggctgcc gacgtcgaac agcttgttct ggacccaacg
     3541 aagatcctgc tcgagccgct tggccgcagc gatcgctggg gtcgcctcct cgttcatcgc
     3601 ccgcgcaagg ccaatcgacg cgctcaactc atccacggtg ccgtaagcct cgacacggag
     3661 acagtctttg gagacctgct ggcctccggc cagccgcgtg ctgccgccgt caccggttct
     3721 cgtatagact ttcgtaattc gcacgatgct tcttactccg gttcggcaac aggtgccggt
     3781 cctaactcac cgttgacgac ccgctgaagg cagagcggac acaaacaatc atgaaaagac
     3841 ttctcgatcc acgtcatctg ctgctcgttc acttcgacct tggcgcacca acaaccgtat
     3901 tggccgcagg taaagccttg cttacagcat tcgcagactg tcgagatagg ccctctcatc
     3961 ccctgctccg atcgctcgtt ctacgccgac attcgtcgcc atgatcccat gcctcggccg
     4021 atcagatggg catgtcgaaa ttcactcgta ccccgccaaa caccgatcgg ataggcgttc
     4081 caaaaaacaa cacttcttcg tatttccggt tgaagatgtt gtccgcacga ccatagatct
     4141 gaaccgtctt gttgacgtca taactgaacg aggcattcca cacttagaaa tgcgggagtg
     4201 agtcgtcatt gctggggtcg ttgaaccgct gtcccacgaa tcgcccttcc agattcgcac
     4261 gcagcgcttc gataggctgg taactcaaaa tggcagacca tt
//
LOCUS       DASSCQ010000216         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1182432, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000216 DASSCQ010000000
VERSION     DASSCQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182432"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1336)
                     /locus_tag="VFH05_13485"
     CDS             complement(209..1336)
                     /locus_tag="VFH05_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679282.1"
                     /translation="MKWTIFLALVLSVAMGSCARTQQGIVGTGTYQVLDLGVHPGFDT
                     SEPSGINSLGQVSGFSIKTSDSSRRAFFYDTPHCQRIDVGAAVPQQASSDAMAINDSA
                     MVVGSFRRADDGRLRAFSFHQGVFRDLGGGEGVEHHALAVNKSGKIVGFESVPTLPTE
                     NAVAYTNGQSVPFPIVGTAYVNRATGISDSGEVTGPLKAAGGQTLGMKYFPNLTRWYQ
                     IRPAASHPQLPDFDRVMQPRAINASGNVTGTIGSTVPHAFLSRNHFELTRDLGTLDSA
                     HPEWFSVGYAINASAHVVGVAQTGSGITAFLHNTITMIDLNTKLSDAPGWRLIGALAI
                     NDKGLIAAQASLNNGPGHAVLLVPVSRVGPVTPLCQPSVFG"
     gene            complement(1380..1883)
                     /locus_tag="VFH05_13490"
     CDS             complement(1380..1883)
                     /locus_tag="VFH05_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679283.1"
                     /translation="MRNPGMSSWQLYGGGLVVAGILTGGCALRASPVQMYEGPSLPKE
                     QVSILRSACKTTPGLTIMIMRIDDKDVRNGCADFALLPGDHQLELSAKQLAPRIDTPM
                     IRSGSAMGAPPSTIGASQDEDLPVIWASSSPLRISCTLRPGQEVTITGNAGTRPDWQA
                     QCQERAR"
     gene            complement(1927..2421)
                     /locus_tag="VFH05_13495"
     CDS             complement(1927..2421)
                     /locus_tag="VFH05_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679284.1"
                     /translation="MKIHCIWMIGLAILAVAGCNGTVGTERVAAERHVHSRPDPVLDM
                     LHRGVIDLNESIDELKREIAELQQLPAVSDPHMQQLYGLDLAAWQLHLQQWVVQRDRL
                     LSSLNFIQQAQASPQDKAAIGNQWSERQAEYLKVIEELRTQRRTIEQKRSDVESQVLR
                     RYFN"
     gene            complement(2462..>2850)
                     /locus_tag="VFH05_13500"
     CDS             complement(2462..>2850)
                     /locus_tag="VFH05_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679285.1"
                     /translation="REVQDVAILSQFPSVQHQKIAELSETSSSENVSKLQRHLLDKAA
                     ELGADAVVFSPSITRTEQRMAYQPVYSPWGYYAPYYGPGPYGYGGPWGYRYGPWGGSM
                     MGYYQSVPVPYLVRSTTVKGTAIRYN"
BASE COUNT          566 a          848 c          837 g          599 t
ORIGIN      
        1 gtctgcgacg tgtcctctgg acgcttcggg catcctgcga ccgttcagat gtggcgttca
       61 attatctaaa ggaggtaata ctttgagcta caacagagga gagactgcgt atatgaaaat
      121 cagtgactcg attcgtggaa gctaaatgca cgtattctat tgctcacatt cggctctcaa
      181 caaaaatgtc aacgcgaatt gccgaacgtc agccgaacac ggagggctgg catagtggag
      241 tgacgggacc aacacggctg acgggcacaa gcaggacggc atgccccggg ccgttgttga
      301 gtgacgcctg cgcagcaatc agacccttgt cgttaatggc tagtgcgccg atcagccgcc
      361 acccgggagc atccgagagc ttcgtgttca ggtcgatcat cgtgatggtg ttgtgaagaa
      421 acgcggtgat tccggagccg gtctgcgcca cgccaaccac gtgggcggac gcgttgattg
      481 catatccgac gctgaaccac tccggatgtg ccgagtcgag cgttcccaga tcccgtgtaa
      541 gctcgaaatg gttccgcgag agaaacgcat ggggtaccgt gctaccgatg gtgcccgtca
      601 cgttcccgct ggcattgatg gcccgaggct gcatgacgcg gtcaaagtcc ggaagctgcg
      661 gatgcgaggc ggccgggcga atctgatacc accgggtgag gttcgggaag tacttcatgc
      721 ccagtgtctg acctccggct gctttgagcg gccctgtgac ttcacctgag tcactgatac
      781 cggtcgccct gttcacgtaa gcggtaccaa cgatcggaaa tgggacagat tgcccattgg
      841 tgtatgccac cgcattttcg gtagggagcg tcggcacgga ttcaaatccg acgattttcc
      901 cgctcttgtt caccgccagc gcgtggtgct ccaccccttc gccaccgccg agatcccgga
      961 acacgccctg atgaaaggag aaggctcgta acctcccgtc gtcagcgcgc cggaaggagc
     1021 cgaccaccat ggcggagtcg ttaatggcca tcgcgtccga tgaggcttgc tgcgggacgg
     1081 cggcgcccac atcgattctc tggcaatgcg gtgtatcgta aaagaacgcg cgccgggatg
     1141 agtcgctggt cttgatcgag aaaccagata cttgtcccaa gctgttaatg ccgctgggct
     1201 cggacgtatc aaatcccggg tgcacgccaa gatcgagcac ttgataggtt ccggtcccga
     1261 caatcccttg ttgggtccgg gcgcaggaac ccatcgccac tgagaggacg agggccagaa
     1321 agatcgtcca cttcatgacc atctcctttg ctttcaacct gaccatacgc gtgccatgac
     1381 tagcgtgccc gctcctgaca ctgcgcttgc caatcaggcc gcgtcccggc gttcccggtg
     1441 atcgtaactt cctgccctgg ccttagcgta caactgattc gcagcggcga tgaggatgcc
     1501 cagatgaccg gcaagtcttc atcctgactc gcaccgatcg tagacggcgg cgcccccatc
     1561 gctgacccgg acctgatcat gggcgtatcg attcttggcg ccagttgctt ggcactgagc
     1621 tcgagctggt ggtcaccggg tagcagtgcg aagtccgcac agccattccg gacgtcctta
     1681 tcgtcgatcc gcatgatcat gatcgtcaag ccgggcgtag tcttacaggc actgcgcagg
     1741 atgctgactt gctccttggg cagcgacgga ccttcataca tctggaccgg gctcgccctg
     1801 agagcacagc cgccggtgag gattcccgcc acgacgagac cgccaccata gagttgccag
     1861 gacgacattc cgggatttcg catgttgttc cctccactcg ctcttgagcg aggacatgtt
     1921 cgacgcctaa ttgaaatacc gccgcagaac ttgcgattcg acgtcggagc gcttctgttc
     1981 gattgttcgt cgctgagtcc tcaattcttc tatcactttc aggtattccg cctgacgctc
     2041 cgaccattga ttgccgatgg cggctttgtc ttgcggagac gcctgcgcct gctgaatgaa
     2101 gttgagcgag gagaggagac gatcccgttg gaccacccat tgctgcaggt gaagctgcca
     2161 ggccgcgaga tccaatccgt acagctgttg catgtgggga tccgagacgg ccggcaactg
     2221 ctggagctca gcgatctcgc gcttcagctc gtcgatactc tcgttgaggt cgatcacgcc
     2281 ccggtgcagc atgtcgagga caggatccgg ccgcgagtgc acgtgtcgtt ctgccgcgac
     2341 ccgctcggtc ccgacggttc cgttgcagcc ggccacggcc agtatggcca gtccgatcat
     2401 ccagatgcag tgaatcttca tagattgtcc tccgactgag cgggcgtcta gtgaatcgtc
     2461 gtcaattata gcgaatcgcc gtgcctttca cggtcgtgct gcgcactaga tacggcaccg
     2521 gaacggattg gtaatagccc atcatcgatc ccccccatgg tccataccga tagccccacg
     2581 gccccccata gccgtaggga cccggaccat aatagggcgc atagtatccc catgggctat
     2641 agaccggctg ataagccatg cgttgctcgg tccgcgtaat cgacggtgag aacaccaccg
     2701 cgtcggctcc gagctcagcc gccttgtcga ggagatgtcg ttgaagtttg gacacgttct
     2761 ccgaggagga cgtctcgctg agttcggcga tcttttgatg ctggacgcta ggaaactggc
     2821 tgaggatggc gacgtcctgc acctcgcgag
//
LOCUS       DASSCQ010000217        15642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_742733, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000217 DASSCQ010000000
VERSION     DASSCQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15642
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742733"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1328)
                     /locus_tag="VFH05_13505"
     CDS             complement(261..1328)
                     /locus_tag="VFH05_13505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET6679286.1"
                     /translation="MAKSWNANAYAAAGPTASLAPITIARRDPAERDVHIEILYCGIC
                     HSDLHTVRDEWKSVMPTVYPCVPGHEIVGRVTRVGSAVTKFKTGDLAAVGCLVDSDRT
                     CGDCQHGLEQFCPNLTLTYNSPDTHLGGVTYGGYSDAIVVDERFVLRVPPQLDLAGAA
                     PLLCAGITTYSPMRHWGVTKGKKVGVVGLGGLGHMAVKLAHALGAYVVVFTTSPNKKD
                     DAIRLGADEVVISRHRDEMKKQAGSFDYILDAVSADHDINAYIDLLRRDGTLTLVGAP
                     EKPLAVAAFSLIFGRRSLSGSPIGGIPETQEMLDFCGTHNITAEVEVIPIQQVNEAYD
                     RLLKSDVKYRFSIDMASLKSA"
     gene            1574..1999
                     /locus_tag="VFH05_13510"
     CDS             1574..1999
                     /locus_tag="VFH05_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679287.1"
                     /translation="MERTILVGLTVLTLLSAVGCSSLTVVTELNNQRFTLDEQPVAHL
                     SARVGGIYLFYYIPLVTGDSEQTDRIRFFRHEPDVERVVHMVTRKSKELGATRLTDLY
                     SWSDSVWASFSLIFWYRWTSVSGNASMAPDVPGSHQTIR"
     gene            2785..3486
                     /locus_tag="VFH05_13515"
     CDS             2785..3486
                     /locus_tag="VFH05_13515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6679288.1"
                     /translation="MTRVKSILGLFLLVLATACSNIQQERKIEFVYLALGASDVMGVG
                     ATPLTEGYAYLVSRDLEEHVPGTFLINLGIPGARIDALTEQVRLAKHFGGEADVATVW
                     VGANDLVHGVGPSRFQSDLRQLLRRLQTSLSKTVVIATLPDLTRLAAFGATPNPAVTD
                     ARVQAFNRAIEVEAPYVGASIVDLFHDTSPDDFAFDEDGFHPTKAGHRRMADLFGKSI
                     FERLGLQSLSNQAPP"
     gene            3610..3948
                     /locus_tag="VFH05_13520"
     CDS             3610..3948
                     /locus_tag="VFH05_13520"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6679289.1"
                     /translation="MPDCRNTRRLAVTGQASVVSLLTEDSEDGTLLDLSRKGARLRIN
                     HLFQRGQKLTLMLNLPWEEPPVQVRLAAVKWVQQDTVGVEFLKVEADDRVSLDVFLAS
                     RSKHQAGSPA"
     gene            complement(4086..4721)
                     /gene="tmpT"
                     /locus_tag="VFH05_13525"
     CDS             complement(4086..4721)
                     /gene="tmpT"
                     /locus_tag="VFH05_13525"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HET6679290.1"
                     /translation="MDANFWHQRWGKNEIGWHETKGNPLLVQHFNELGLEKGSRIFIP
                     LCGKTLDISWLHAQGYRIAGAELSKVAIEQLFTELDLQPNISALGEGEQWSANHIDIF
                     VGDIFALSTETLGHVDGIYDRAALVAFPEDMRNRYARHLIEITAKAPQLLICYDYDQR
                     CIDGPPFSIPDQEVKRQYASAYQLKLLSSIEVPGGLKGKCPANEKAWLLSN"
     gene            complement(4747..5373)
                     /locus_tag="VFH05_13530"
     CDS             complement(4747..5373)
                     /locus_tag="VFH05_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679291.1"
                     /translation="MKDVEARSDWVFGLSLIQVILLLFFAATLIYVTQNGDVSGREPS
                     PAFGETNHHDVSLRARLDAATEKNHALEQELGDMSALVDELKVMVGAKAPSKEGFREA
                     IETLKRGYALCQKDDNTLIEATIRQGAENVQVIGEIPSDLYVNLAKGNSTSDLDEIVS
                     FIQDVYEYEKDHKCRFNYRLKYATDNDYRKAREGYEKYFYPEKMIKVG"
     gene            complement(5360..6604)
                     /locus_tag="VFH05_13535"
     CDS             complement(5360..6604)
                     /locus_tag="VFH05_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679292.1"
                     /translation="MHRLPTIPFERPMPVDELEDHPPSSDDSALTHLKLATAWLCTTL
                     AGVAAIAYATSSETSPPLTMVTLAVLLVAYAGYGYFIPRKNSIQYADSLYYMGFLWAI
                     FALIAMFVIWPAPKLTTEAVLTSFGYALVTTFCGMFLRLVMIQFRDDTAPDRIEVAEA
                     MIQRRIAALIQEINEATLEMATFRDRAAGDLGGTMRDLVQSLTAVREKIAEQHRTMAK
                     AMSDGFESSLKDILGRLSAIQIPQEYLTGEVAKLASTLGRQGESFQTAAQRLETSLLR
                     AAESVTSFGDSLHGSEGAKQVGMAVNDLSMKIKERTEQFLKMTTTFEQSRADLDNQLI
                     NLQSLRSAASMVATRLSSFEHELLELSSASLSAEVKTGLMSVQQAIRSSLEGSKAIES
                     MMRDVLLLMRQRVTEEHSGERR"
     gene            complement(6916..7119)
                     /locus_tag="VFH05_13540"
     CDS             complement(6916..7119)
                     /locus_tag="VFH05_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679293.1"
                     /translation="MNDTDLSRILSLIITVLTDPYDQFIEKLRTDGRITDEEVQALHL
                     ALKEQIPKLSELIKPPQDHSPET"
     gene            complement(7160..7483)
                     /locus_tag="VFH05_13545"
     CDS             complement(7160..7483)
                     /locus_tag="VFH05_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679294.1"
                     /translation="MTPNQTTKSWKRIPDGTRVRLRENAQEGVIDGLTELVIGPARNP
                     DGRTQYRINLGDPERMLVSEDALLIVVDKEGLVFILKEKAEYRRLITEQLRGGFSADR
                     FVPLN"
     gene            complement(7518..8774)
                     /locus_tag="VFH05_13550"
     CDS             complement(7518..8774)
                     /locus_tag="VFH05_13550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6679295.1"
                     /translation="MDTSVQLSFRDIGVSEALVNDLAKAGLSSPTPIQAQAIPAALNG
                     RDVIGCAQTGTGKTAAFVIPVIERLARLPKGEPQALILAPTRELALQILKTIETLGHR
                     LGISATVIVGGADMQAQIRGLRQRPDILVATPGRLLDHMWNGTVNLLPIKILVLDEAD
                     RMLDMGFAPQISQILTALPEERQTLLFSATLPSDLTQFVQASVNDPVEVMVTPSATTA
                     DGITQALHYTTHTGKQELLLSLLKQEKETVLIFTRTKHRAEQVGHVLGRAGHRVGILH
                     GDRSLSQRRSALDGFKRGTFRVLVATDIAARGIDVANIGHVINFDLPYCPEDYVHRIG
                     RTARMKATGRATSFITAEDPQQLRAIERLLGHAVPLAAGSPQPVSIQPSQTGSRRHGR
                     PFSGRTARSRSHVHENHPMTQGSVLQ"
     gene            9155..9493
                     /locus_tag="VFH05_13555"
     CDS             9155..9493
                     /locus_tag="VFH05_13555"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6679296.1"
                     /translation="MPNKHMSLDRIFSALADPTRRSVLQRLSTRPAPVSDLAEPFDMA
                     LSSFMQHLDVLEDCGLVRSQKTGRVRTYELRLPSLRAAEKWLAKHRIVWETRLEQLDD
                     YLITMEEQTR"
     gene            9490..10020
                     /locus_tag="VFH05_13560"
     CDS             9490..10020
                     /locus_tag="VFH05_13560"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6679297.1"
                     /translation="MTRSIVQPDPRLDLIFERIVDVPQELVWKAWTTPEHLKPWFTPS
                     PWKTVDCEIDLRPGGLFRTVMRSPEGQEFPNVGCYLEVVKNEKLVWTNALLPGYRPAP
                     PPAADAPACDTFPFTAILMFERHQKGTKYTALVIHRDEAGRKQHEEMGFHEGWGKALD
                     QLVEYSKRQLTGADRR"
     gene            complement(10564..11262)
                     /locus_tag="VFH05_13565"
     CDS             complement(10564..11262)
                     /locus_tag="VFH05_13565"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET6679298.1"
                     /translation="MQQTATAVNVHVSEAVGDVEALLLRPNNARSLLVLAHGAGAGMH
                     HTFMESLARELAGVGVGTFRYQFPYMQQRRGRPDAPALLTATVQAAVKAASKAASDLI
                     LLAGGKSLGGRMTSLAFAEGPSTLQAGTAPVRGLVFFGFPLHPPGRPGTQRADHLTAV
                     QMPMLFLQGTRDTFADLTLLRPVCAKLAPHATLHVVDAADHSFHVLKRSGTTDAEVIT
                     ELARTVASWAHTLG"
     gene            complement(11367..12080)
                     /locus_tag="VFH05_13570"
     CDS             complement(11367..12080)
                     /locus_tag="VFH05_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679299.1"
                     /translation="MLYARKLSGCWLVLALLFSFADANLLASAGQNAASDRSPSVLVL
                     DPPSLKRPPVAEGIVDRYLLQPRGDVSGLLLRDGAQMHITNRAAQELINAVQPGDHVR
                     VHGRRASDSPLVKPDVIVNVTDGTTFTVPYRLDLPIPPPEDRPTVNEMKAAGKITVLL
                     YDPLKGVVNGVVLSDGTQVRLPPDVGERFHASLHPDMEVEVEGFGTSTRHGNALEATA
                     IGQKGRPLTHLDASIQQLR"
     gene            12233..12766
                     /locus_tag="VFH05_13575"
     CDS             12233..12766
                     /locus_tag="VFH05_13575"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HET6679300.1"
                     /translation="MTYNRQQRWVTSLALALTITGTLWACSSVPRRYIWIAEPGVTLT
                     MLSTHQQSYVGKVVLLGGTIIEQEDHEQFVLLRLKNRPLDQDYKPHRPADTASSEGGF
                     YWVMMAKGQVPLKYQDWARATVVGRVTGEYRLRSEPVLTLLYMRGWGASGNHAGVWEN
                     IDPNYVPGVPGSIDMRN"
     gene            12854..13315
                     /locus_tag="VFH05_13580"
     CDS             12854..13315
                     /locus_tag="VFH05_13580"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET6679301.1"
                     /translation="MTALEFKVPPVALTLLFALMMWLTSTYEAPLPVPLPWRRAIAFS
                     VCCAGLVIGLGGVVAFRRAKTTVNPITPEATTTMVTSGLYRFSRNPMYLGLLLVLIGW
                     AIFLSNLLAFALLPLFVLYMNRFQIIPEERVLSGKFAHQFTAYKKTVRRWI"
     gene            13402..14130
                     /locus_tag="VFH05_13585"
     CDS             13402..14130
                     /locus_tag="VFH05_13585"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HET6679302.1"
                     /translation="MQWLHGMLLITLALSISGCPATKQARTVERLGFLKDLYPRMTEG
                     DEHAGESLLIYKNPKVPNIPPNSYTKIMLDPVLFFRGPQSKMQGISQHQAQLMADTFY
                     AVIYQEVSKDYEMVDKPGPSTLRVQVAMTHLEESWPMLDVVSSIPAPMNALAAGSLLK
                     NVATGKPAFKGEAVIEAKVMDAQTGEVLRAIVDRRVGTKRLHAESFNSWSDVYESLRY
                     WAENGRYQLCKARGQLTDCPKPRA"
     gene            complement(14245..14652)
                     /locus_tag="VFH05_13590"
     CDS             complement(14245..14652)
                     /locus_tag="VFH05_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET6679303.1"
                     /translation="MTHATTRRQSRTCFWVLGLITVLLSGSMADAKSSSAPGGKTGKE
                     TPCDYRAHPTITAVTPTQVKPGQRITISGKNFGSKRCFHSVSFGAKSTDKWTYVSPTT
                     VEATVPSLLPGAVPVTVSTEAGTSQYKLDVQAK"
BASE COUNT         3403 a         4385 c         4327 g         3527 t
ORIGIN      
        1 gcgatgggaa ccggtcgaca cgcgctcaga gcgacggaaa acaccaccgc acccatcgcg
       61 ctgcccatga gcttcgtcca cccgtgaatc atgctctctc ggatcttccc tgccattaac
      121 aacgattgtg ccagactgag ccccagatgt cagcaaaggt ttcgcggcgc atggcggacc
      181 tgatggtctg ccaaactaat ggagttgact ttgctgaaga gaaatacgtg gtggttcgat
      241 catacggtca cggactccgg tcatgcggat ttgagcgaag ccatgtcgat ggagaaacgg
      301 tatttcacat ccgacttcag cagtctgtcg taggcttcat tcacttgttg aatagggatg
      361 acttccacct ctgctgtgat attgtgcgtc ccacaaaagt cgagcatctc ctgcgtctct
      421 ggaataccgc cgatcggtga gccggacagg ctccggcgtc cgaaaatcaa actgaaggca
      481 gcgaccgcga gcggcttctc aggcgcgccg acgagggtga gggtgccatc gcggcggagc
      541 aggtcgatat aggcgttgat gtcgtgatcg gccgagaccg catcgaggat gtaatcaaag
      601 ctacccgcct gcttcttcat ctcatcacga tggcgggaga tgacgacctc atcagctccc
      661 aggcggatcg catcgtcttt cttgttggga gatgtggtga agacgactac ataggcccct
      721 aacgcatgag cgagcttcac cgccatgtgg cccaaccccc cgaggccgac gaccccgact
      781 ttcttgcctt tggtgacgcc ccaatggcgc atgggcgaat acgtggtgat ccccgcacaa
      841 agcaggggcg ccgcgccggc gagatcgagc tgtggaggga cacggagaac aaaacgttca
      901 tccacgacga tcgcgtcgga atagcccccg taggtgacgc cgccaaggtg cgtgtccggc
      961 gaattgtagg taagcgtcag gttcggacaa aattgctcga ggccgtgctg acaatcgccg
     1021 cacgtgcgat cggagtcgac caagcagccg acagcagcca gatctccggt cttgaacttg
     1081 gtgacagccg agccgaccct cgtcacccga ccgacgatct catggcctgg cacgcagggg
     1141 tagacggtcg gcatgacgct cttccattcg tcgcgtaccg tatggagatc ggagtgacag
     1201 atgccgcagt acaggatctc aatgtggacg tcgcgttcag cgggatcgcg ccgcgcaatg
     1261 gtgataggtg cgagcgacgc agttggaccg gcggcggcgt aggcgttggc attccaactt
     1321 tttgccatgc tgtatctcct tcctacaggc gttcatgtgg actaaggact atccgatctc
     1381 tggccttatt gtcgagagtg tagaaccgtg ctagggcata tttgggaacc gaccatccaa
     1441 ttcaaggggg tgaatgacgc tctttcccag gggtgagtgg ttctaaccag caagaggatt
     1501 ggactggcgc agttccgccg cctaaaactt tttggggtaa atggacaata tctatagaga
     1561 ttaggaggtg aacatggaac gtactatcct tgtcggactc accgtgctta ccctgctctc
     1621 cgcagtgggt tgttcgagtc tcaccgtcgt cacagaactt aacaatcaac gattcacgtt
     1681 agacgagcaa ccggttgctc atctgagtgc gagggtggga ggtatttacc tcttctatta
     1741 tattccgctt gtgaccggag attccgagca aacggatcgt attcgcttct tccgtcacga
     1801 gccggacgtc gagcgtgtcg tgcacatggt gacaaggaag agcaaggaac ttggcgccac
     1861 acgcctcacc gatttgtatt cttggtcgga tagtgtgtgg gcttctttca gtctgatctt
     1921 ctggtaccga tggacatctg tttctggcaa tgcttcaatg gcacccgatg ttcctggctc
     1981 acatcagacg atccgatagg gacagcaacg aatgaacaaa tggacagtgc tgtctctccg
     2041 cattgcttgt tgccgttccc ttctcgatgt attcaaaggc gagtgcatcc agggtggaac
     2101 gcaagaatgt tgcttctgct ttccacatcc cacgaggtgc gatatataga ggaaagagtc
     2161 ctccatatta ctcacagctc tgggggaagg aacatggcac tatcaggaag atggcgacga
     2221 ttgtggaatt ttcgacgctg gaagaaggcc accaccccga tgttactcaa tgccgaaaca
     2281 ggggaactca tccctctttc cagctatacc gctcctccgg aaggcaagtg gatgcttcgc
     2341 cgtccactga cactgcctga tagtccggta cctctctttc tcgaggttcg cggtatccca
     2401 acgcggaaaa agcgcagtga cgtcaaacct gctgcgccgt ctgcgtagac agccaatacg
     2461 agcgcctgta cgccttgctg tggcaggtgt tatcctctcg tctgctcgct tgtaatcgat
     2521 aaagttcctc gtcagtcccc ccgtctccag atcgcttcgg tcactccttg gcacgtccat
     2581 tccggtggcc atcgagcaga tcgcttcgct tcgctcacct cgcggttacg ccgccctcta
     2641 atcctccttg tctacaattt ttctccgttg tccggcactt catgatggaa cacaggggcc
     2701 tgtagacgta gaatgcagcc atgtcctacc tgacgcagat tggtttacaa gagcgcaatg
     2761 gctgagcggg aagaggagac aggaatgacc agagtgaagt cgatccttgg actgtttctg
     2821 ttggtgttgg ctacggcctg cagcaacatt cagcaagaaa gaaagattga atttgtgtat
     2881 ctggcgcttg gagcaagcga cgtgatggga gtcggagcaa cgccgctcac cgaggggtat
     2941 gcctatctgg tcagtcgcga tttagaggaa cacgttccag ggaccttctt gatcaacctt
     3001 ggtatcccgg gagctcggat tgatgcgctg accgagcagg ttcgcctggc gaagcacttc
     3061 ggcggtgaag ccgacgtcgc taccgtgtgg gtcggagcca atgaccttgt ccacggggtg
     3121 ggcccttcac gatttcaaag tgatctcaga cagctccttc gacgccttca gacttcgtta
     3181 tctaagactg tggtgatcgc caccctgccc gacctcacac gactcgctgc gtttggtgca
     3241 acacccaatc cggccgtcac cgacgcacgt gtccaagcct ttaatcgtgc catagaggtg
     3301 gaagcgccgt atgtgggtgc ctccatcgtg gatctgtttc acgacacgtc gcctgatgat
     3361 tttgcctttg atgaagatgg gtttcatcct acgaaggccg gccatcgacg catggccgat
     3421 ctgtttggga agagcatatt cgagcggctg gggttgcaga gcctctcgaa ccaagctcct
     3481 ccataatgcg gcacgcccag gtcagagccc ggtttctcca tgcacaatat ctaggacatt
     3541 ccctctactc cggcccttgc tgccgcacca cgggagagcc tacaatatgc cactgggagt
     3601 gccatatcga tgcccgactg tcgcaacacg aggcgtctcg ccgtcacagg ccaagcctct
     3661 gtcgtatcgc tgctgacgga ggactctgag gacggaactc tgctcgatct ctcccgcaaa
     3721 ggtgcccggc tgcggatcaa tcacctcttt caacgcggtc agaagcttac gcttatgctg
     3781 aaccttccct gggaagagcc accagtgcaa gtgcggctcg ctgcggtcaa gtgggtacag
     3841 caagacacgg tcggcgttga atttctcaaa gtcgaagctg acgaccgtgt ttccctcgat
     3901 gtctttctcg cctcgcgttc caaacaccag gcaggtagtc ctgcgtgaga ggctgatctg
     3961 gcgcgtcgca tgcggatgag ccaatccctc tctaggcaac cttcttctcg tcctgcctca
     4021 atgtcctatc caggaaatat gttcacggaa aagcgaggtc gattagagag ggtctcggta
     4081 gggcatcagt tgctgagcag ccaagccttt tcgttcgcgg ggcattttcc cttcaatcca
     4141 ccagggacct ctatgctgga gagcagcttg agctgatacg cagatgcgta ctggcgcttc
     4201 acctcctggt caggtattga aaaaggagga ccgtcgatgc agcgctggtc gtagtcgtag
     4261 caaatgagca gctgcggagc ctttgctgtt atctctatca ggtgccgggc atagcgattc
     4321 cgcatgtctt cggggaaggc cacgagagct gccctgtcat atattccatc aacatgacca
     4381 agcgtctcgg tggacaacgc aaagatgtcc ccgacaaaaa tgtcgatatg attcgcgctc
     4441 cactgctctc cttcgcctag tgctgagatg ttcggttgca ggtcaagctc ggtaaacaat
     4501 tgctcaatgg cgactttact cagttcggcg ccggcaatcc gatacccttg agcgtgcagc
     4561 caggagatgt ccagagtctt cccgcacaag gggataaaga tccggctgcc tttttctaga
     4621 cccaactcgt tgaagtgttg aaccagcagt ggattgccct tagtctcatg ccacccaatt
     4681 tcattctttc cccacctttg gtgccaaaaa ttagcatcca tacgcagata cagtcgtcaa
     4741 cagcggtcaa ccgaccttga tcatcttctc aggataaaaa tacttctcgt atccctctct
     4801 cgcttttcta tagtcgttat cggttgcata cttcagtcgg taattgaatc ggcatttatg
     4861 gtccttctca tattcataca cgtcctggat gaacgacaca atttcgtcga gatccgacgt
     4921 gctgttcccc ttggccaaat tcacatagag atcggacgga atttctccga tcacctgaac
     4981 gttttccgct ccttgacgaa tggtcgcttc gatcagcgtg ttatcatcct tctgacacag
     5041 ggcatatccg cgcttgagcg tttcgattgc ctctcgaaag ccttccttag atggagcttt
     5101 ggcgcccacc atcaccttca actcatcgac caacgcgctc atatccccaa gctcttgctc
     5161 caacgcatgg ttcttctccg ttgccgcatc gagccttgcc cgaagactga catcgtgatg
     5221 attcgtttca ccgaatgctg gggacggctc tcgtccgctg acatccccat tttgtgtcac
     5281 atagatcaac gtcgcggcga agaacaggag gagtatcact tgaatcagtg acaagccgaa
     5341 cacccagtct gatcgcgctt caacgtcttt cacccgaatg ctcctcggtg actcgctgtc
     5401 tcatcaagag caggacatca cgcatcatgg actcgatggc tttgcttcct tcaaggctcg
     5461 aacggatagc ctgctgcaca ctcatcaggc cggttttgac ttcagccgac aacgaagcag
     5521 acgataactc gagcaattca tgttcgaatg acgacaaacg tgtggcgacc attgaagccg
     5581 ccgatcgcag agattgcagg ttgatcagct gattgtccag gtcagcccgg ctctgctcga
     5641 atgtggtggt cattttgaga aattgctcgg tcctttcttt gatcttcatg gacagatcgt
     5701 tgacggccat tccgacttgc ttcgcgccct ccgatccatg caaggagtca ccaaacgacg
     5761 tcacgctttc ggctgcacgc aacaaactcg tctcgagtct ctgcgccgcc gtttggaagc
     5821 tttccccctg cctgcctaat gttgaagcaa gtttggccac ctctccggta agatattctt
     5881 gaggaatttg gattgccgag agccggccga gaatgtcttt gagagacgac tcaaagccat
     5941 cgctcatcgc cttggccatc gtccggtgtt gctcagcgat cttctcgcgg acagctgtca
     6001 aagactgcac gaggtcccgc atcgttccac cgagatcacc ggctgctcga tctctgaacg
     6061 tcgccatttc taaggtcgct tcgttgatct cttgaatgag agccgctatg cggcgctgga
     6121 tcatggcctc ggcaacctca atccgatccg gagccgtgtc gtctcgaaat tggatcatca
     6181 ccagccgaag aaacattccg cagaaagtgg tgaccagcgc atatccgaag gacgtaagga
     6241 ccgcctcggt cgtgagcttc ggagctggcc agatcacgaa cattgcgatc agggcaaaga
     6301 tcgcccagag gaaccccatg tagtagaggg agtcggcata ttgaatgctg ttcttccgtg
     6361 gaatgaaata cccgtaaccc gcataggcga ccaacagcac ggcaagcgtc accattgtta
     6421 gaggaggcga ggtttctgag ctcgttgcat aggcaatggc ggcaacacct gccagcgtcg
     6481 tgcacagcca ggccgtagcg agttttagat gggtcagagc cgaatcgtcg cttgatggcg
     6541 gatggtcttc aagttcatca accggcattg gcctttcgaa cggtatggtg ggaagccgat
     6601 gcataacttc ctccccatgg ttatatgcaa ggtttctgtt cggccttatc ttctggccta
     6661 gccctagctc ccataactgg ggattaccct agcagcgtgt gaacgtagtc tctgcagcga
     6721 tcaatagcat caaacaaaat agtgtcacct tttcaatgca ttggtactcg ttccttaagt
     6781 tattgtttaa gcgatacccg aagctcgatg tgatccttcg ccgtcttctg ctgcttttct
     6841 caaagccaca gaacgggtgc gccgagatat tgttcgatcc attctgagcg gcgggatacg
     6901 acttgcagtt cgtcgtcagg tttctggcga gtggtcctgg gggggcttta ttaactcgga
     6961 caacttcgga atttgctctt tgagggccag gtgaagcgct tgcacttcct cgtcggtgat
     7021 tctgccgtca gtacgcagtt tctcaatgaa ctgatcatag ggatctgtca gcaccgtgat
     7081 gatcaagctg aggatgcgac tgaggtccgt atcattcatg catctgattc ttgatcatcc
     7141 ggagtggctg aagttctctt caattcaaag ggacaaacct gtccgctgaa aatcccccgc
     7201 gcaactgctc ggtgataagg cgtcggtact cggccttctc cttcaggatg aagaccaacc
     7261 cttccttgtc gaccacgatc agcagcgcat cttccgacac gagcattcgc tcaggatcgc
     7321 cgagattaat gcgatactgc gtccgcccat caggatttct tgcgggcccg ataactaact
     7381 ccgtcagtcc atcgatgacg ccctcttggg cgttctccct gagacgtacc cgagttccgt
     7441 cgggaattct tttccaactc ttcgtcgtct gatttggcgt catgatgccc cctttcacga
     7501 tgtagtacgc tgatccgcta ttggagaacg ctgccctgag tcatgggatg gttttcatga
     7561 acgtgcgagc ggcttcgtgc tgtacgccct gagaagggcc ttccatgtcg ccgagatcct
     7621 gtttggctcg gctgaataga gacaggctgc ggacttccgg cggccagcgg caccgcatgg
     7681 ccaagaaggc gttcgatcgc acggagctgc tgcggatctt cggctgtaat aaagctcgtg
     7741 gcccgtccgg tcgctttcat ccgggccgtc cggccgattc gatgaacgta atcctcaggg
     7801 caatacggta aatcgaaatt gattacgtgg ccgatattgg ccacatcgat accgcgtgcc
     7861 gcaatatctg tcgcgacgag cacgcgaaac gtcccccgct taaaaccatc aagcgcgctt
     7921 ctgcgctgtg acagactgcg gtcgccgtgc agaatgccga cacgatgtcc tgcacgaccg
     7981 agcacgtgcc ccacctgctc tgcccgatgc ttggtccggg taaaaatcag gacggtctcc
     8041 ttctcctgtt tcagcagaga aaggagtagc tcctgcttgc cggtatgggt cgtatagtgc
     8101 aacgcttgcg tgattccgtc cgctgttgtg gccgacggcg tcaccataac ctccaccggg
     8161 tcattgacgc tcgcttgtac gaactgcgtc agatcggatg gcaatgtggc ggagaacagc
     8221 agtgtctgcc gttcttccgg cagtgcggtg agaatttgac tgatctgcgg agcaaatccc
     8281 atgtccagca tccgatcggc ttcgtcgaga accaggatct tgataggaag caaattgacg
     8341 gtgccattcc acatgtgatc gagcagccgc ccgggcgttg ccacgagaat atccggtcgc
     8401 tgacgcagcc ctcgtatctg ggcttgcatg tccgcgccgc ccacaatgac ggttgcggaa
     8461 attccaagac ggtgaccgag tgtttcaatg gtcttgagaa tctggagcgc gagctctcgg
     8521 gtcggagcca agatcaaggc ttgcggctca cctttcggca atcgagccaa ccgttctatg
     8581 acggggatga caaatgcggc ggtttttccg gtccccgttt gagcgcaccc gatcacgtca
     8641 cgcccgttca gcgccgcagg aatcgcctgg gcttgaatcg gagttggaga tgaaagccct
     8701 gccttggcaa gatcgttgac caatgcctcg gacacaccaa tgtcgcgaaa acttaattgc
     8761 acagacgtat ccactacaca atcctttcag tgtgatcgca tgatgacggg atcagagaac
     8821 cgaggggaga aacaaccagg aagggtgccg ctccagactg gacctggtcg cgatgcgatc
     8881 gcgaagtccg tgatactctg catgtcccag gccggactca tacgagggaa cagccggaca
     8941 cagcatacag gacgcgcacg tcgcagtcaa tgtatggcga acttctccgt gcctcttacg
     9001 ctcatcccga tgtgggtctc atcgggccat gaggcagggc cggcgaggaa cgtccgcgtg
     9061 cgtggcagca tctacgctcc tacagctggg tcctgcctgg ttcactccat ttcggccgtt
     9121 gacttcccct tcgtgaaact ctacagttag gcacatgcct aacaaacaca tgtctctcga
     9181 tcggatcttt tctgccttag ctgacccgac cagacgttcc gtacttcaac ggctcagtac
     9241 gcgaccggct ccggtcagtg atcttgcgga gccgttcgac atggcgctgt cgtcattcat
     9301 gcagcacctg gacgtcttgg aggactgtgg cttggtccgc tcgcaaaaaa cgggtcgtgt
     9361 gcggacctat gagctgagac taccctcact gagagcagcg gaaaaatggc tggcgaagca
     9421 tcgcattgtg tgggaaaccc gccttgagca attggacgac tatctcatca ccatggagga
     9481 gcagacacga tgactcgctc gattgtacaa cccgatcccc ggttggacct catattcgaa
     9541 cgcattgtag acgtcccgca agaactggtg tggaaagcct ggaccacgcc cgagcatctc
     9601 aaaccctggt ttactccgtc cccgtggaaa acggtcgact gcgaaatcga tcttcgcccg
     9661 ggcgggctgt ttcgaacggt catgcgctct ccggaaggac aggagtttcc caacgtgggc
     9721 tgttatctgg aggtcgtcaa gaacgagaag cttgtctgga ccaatgcatt actgccgggc
     9781 taccggcccg ctcctccacc cgcagcagat gcgcctgcct gcgacacttt tccgttcacg
     9841 gcaattctga tgtttgaacg ccatcagaaa ggaacgaaat atacggcgct ggtgattcat
     9901 agggatgagg cgggacggaa gcagcacgag gagatggggt tccatgaagg ctggggcaag
     9961 gctctcgatc agttggtcga atattcaaag agacagctca cgggcgcaga tagacgatag
    10021 cacgggatgg gtcaaaatcc cgttagctcg atatcagccg ttatgactgg cggacatttc
    10081 cggtggagca taaagaaaaa tattcgtgat cacgtcagcg gtcttcgcgt acacttgcca
    10141 gcgcccgtca tgaccgtagc caaggagctc caatggggaa gccgcaaatc gggggcctag
    10201 ggttattgat tgcgctcgtg gccgtgttga gcactgaaat ggtgttggcg cggctgattg
    10261 atacctccac caataatcgg ccatcgcctg atcggatgag tgcaatcgtt cacgacagat
    10321 cagccgcatg cggcaccacc acaaggccag agtttcaaca ggacgacgac gagccgcgag
    10381 gtcaggtcga tggcgtcatg gtggctgaag acatgaggtg tcgccagtgc catgggaaat
    10441 gctcagccga tagtttgaga tgccggagcc agtgcgcaag cgacagcacc tgtttggttc
    10501 actgtgaaga gagaacgcga aattgcgaat ctatgtgcaa gcagatcttc caatgccagt
    10561 gagctaaccg agtgtatgag cccacgaagc caccgtgcgc gcgagctcag tgatgacctc
    10621 agcgtccgtc gtccccgatc gtttcagcac atgaaacgaa tgatcggcgg catccaccac
    10681 gtgtaaggtg gcatgaggcg ccagtttcgc gcagaccgga cgtaacaggg tgagatcagc
    10741 aaacgtatct cgggtgccct gtaaaaacag catcggcatc tggaccgccg ttaaatgatc
    10801 ggcgcgttga gtaccgggac ggcctggtgg atgtaaagga aacccgaaga agacgaggcc
    10861 acgcaccggc gccgttcctg cctgcagagt tgacggtccc tcggcaaacg ccagcgaagt
    10921 catccgcccg cccagcgatt tgcctcccgc caacaagatc aaatcggatg cggcttttga
    10981 ggcggctttc actgccgcct gcaccgtcgc agtcaataga gcgggggcgt cgggtctgcc
    11041 ccgtcgctgc tgcatgtagg gaaactgata gcgaaaggtt cccacaccga cgccggcgag
    11101 ttcacgcgcg agactctcca tgaaggtgtg atgcatcccc gcccctgccc cgtgtgctaa
    11161 gaccagaagc gatctcgcat tgttggggcg gagcagcaag gcctcgacgt cgccgaccgc
    11221 ttcggacaca tgcacgttca ctgccgtcgc agtctgttgc aaattactac tggacaatcc
    11281 attccctttt ttcgcatttt gagggtcaga acttcagccg gatcgcgcac gagcgtgata
    11341 gcaaggattt agcccggtaa gaccacttag cgaagctgtt gaatcgacgc atcaagatga
    11401 gtaagcggcc ggcctttctg cccgattgcc gtggcctcca gtgcgttccc atggcgggtt
    11461 gaagtaccaa agccttccac ttcgacctcc atgtcagggt gtaaggacgc gtgaaatcgc
    11521 tcccctacat ccggaggcaa acggacctgt gtcccatccg acaggaccac gccgttcacg
    11581 acgcctttca agggatcata gagcaacacg gtaattttcc cggcagcttt catttcattg
    11641 acggttggtc gatcttccgg tggagggatg ggaagatcga gccggtaggg caccgtgaat
    11701 gtggtcccgt cggtgacatt gacgatcacg tcgggtttca cgagcggtga atccgaggca
    11761 cgtcgtccat gcacccgcac atgatcacca ggctgaaccg cattaatgag ctcttgggca
    11821 gcccgattcg tgatgtgcat ttgtgctccg tcgcgcaaca acaagccact tacatctccg
    11881 cgaggctgca acaaatagcg gtccacaatc ccttcagcca cgggaggccg tttcagcgag
    11941 ggaggatcga gcacgagaac cgacgggctc cgatcagaag ccgcattctg ccccgccgaa
    12001 gcgagcagat tcgcatccgc aaagctgaac agtagggcaa ggaccaacca gcaccctgac
    12061 agctttcgtg catacagcat gtggtcacct gaagacgttg agatcgtatg actattgtgg
    12121 ggcaagatgc tggaaggcat caagaggaag ctccccgtag gaaaactaga ccggcttacc
    12181 cataaacacg taagatgcgc cagcgcttag ccaccggaga cactttccga tcatgacata
    12241 caatcgacaa caacgatggg ttacctccct tgcattggcc ttgacgatca caggcacgct
    12301 ttgggcatgt tcgtctgtac ccaggcggta tatttggatc gctgagcctg gtgtgacgct
    12361 caccatgctg agtacgcatc aacagtccta cgtcggaaag gtcgtgctgc tggggggaac
    12421 tattattgaa caggaagacc atgaacagtt tgtcttattg cgactcaaaa accgcccact
    12481 ggatcaggac tacaagcccc atcgccccgc agacacagca agctcggagg gcgggttcta
    12541 ctgggtcatg atggcgaaag ggcaggtccc cctcaaatac caagactggg cgcgcgcaac
    12601 ggtggtcgga cgggtaaccg gcgaataccg actcaggtcc gaaccagtgc tcacactgct
    12661 gtacatgcga ggatggggcg cgagcgggaa tcacgccgga gtatgggaga acatcgaccc
    12721 gaattatgta ccgggggttc caggtagcat cgacatgagg aattaggcgc catgcgggag
    12781 tgcacgtatt caccctttgc gtgacggttc ctctgacgtt tcattgcagc tgcgctctta
    12841 gccgagggca tgaatgactg ctttagagtt caaagtcccg ccggtggcgc tcacgctgct
    12901 cttcgctttg atgatgtggc tcacctcaac atacgaagct ccacttcctg tccccttgcc
    12961 ctggcgacgg gccatcgctt tctctgtttg ctgtgccgga ctcgtgattg gcttgggtgg
    13021 agtcgtggcc tttcgccggg caaaaaccac agtcaacccg atcaccccag aagcgacgac
    13081 aacaatggtc acttccggcc tgtaccgctt cagccgcaat ccgatgtatc ttggccttct
    13141 cctcgttctt atcggatggg cgattttttt gtccaacctg ttggccttcg cgctgcttcc
    13201 tctgttcgtc ttgtatatga accgctttca aatcattccc gaggaacgag tcttatctgg
    13261 caagtttgcg caccaattca cggcatacaa gaagaccgtt cgccgttgga tatgatgctc
    13321 atgaaatcgg ctgacctgtg ccatgaatga tgtgtccatc acctagcggt ggagccccca
    13381 ataggaaccg aggaggaagg tatgcaatgg ctgcatggga tgttattgat cactttggct
    13441 ctctcgatat ccggatgtcc agccaccaaa caggcacgca cggtggagcg gttaggattt
    13501 ctgaaggatc tctaccctcg catgacggaa ggcgatgagc atgcgggaga gtcgttgctg
    13561 atttataaga atcccaaggt gcccaatatc ccaccaaaca gctataccaa gatcatgctc
    13621 gatccggtgt tgttctttcg cggcccgcaa tcgaaaatgc agggcatctc tcagcatcag
    13681 gctcagctca tggccgacac gttttatgcg gtgatctatc aggaagtctc aaaggattat
    13741 gagatggtcg acaaacctgg cccgagcacg ctgcgggtgc aggtcgccat gacgcatctg
    13801 gaagagtctt ggcctatgtt ggatgtcgta tcgtccatcc ctgccccgat gaatgcgctt
    13861 gcggcgggat cgctgctcaa aaacgtggca accggcaaac cagcatttaa gggcgaagca
    13921 gtgatagaag cgaaggtcat ggatgcccaa accggagagg tgttgagagc gatcgtcgat
    13981 cggcgggtcg gcacgaaaag actgcatgcc gagtcgttta actcatggtc tgacgtgtat
    14041 gaatcgctac gatattgggc tgaaaacggc cgctaccagc tatgcaaggc gcgcggccag
    14101 ttgaccgatt gcccgaagcc tcgtgcgtga cagccgtgaa gtggaatcat gatcgaaggc
    14161 accactttta gagagccgga ccttttccac ttctctctca tcgggagcca gaggtcacac
    14221 tctggctccc tgtaatggcg gcggctactt ggcttggacg tcgagcttgt actgcgacgt
    14281 tccagcctcg gtggagaccg tgaccgggac ggcgcctggc agaagactgg gcactgtggc
    14341 ttccacggtc gttgggctca cgtacgtcca cttgtcggta ctcttggcgc caaatgacac
    14401 actgtggaaa caccgcttgc tcccgaaatt cttcccgctg atcgtgatcc tctgacccgg
    14461 cttgacttgg gtaggcgtga ccgccgtgat ggtcggatgg gcgcgatagt cgcacggcgt
    14521 ctccttcccc gtcttaccgc ccggcgccga agacgatttg gcgtcggcca tcgacccgga
    14581 cagcaacaca gtgatgagac ccaggaccca gaagcatgtg cggctctgcc ggcgcgtcgt
    14641 cgcgtgtgtc atgggtgtgt ctccttcctc ctttactgaa caaagacttg ccttactcta
    14701 agaagtgtag cagaggagat tcggtgcaaa acctaagtgc gcggacttgc ttgagaaatg
    14761 aaactctagc taaaaagaac gccggcggcg ctatatggct gggtgctctg ttcgactact
    14821 tgtgtaagat ccttcgagga agcatcggcc ggagcatgga gcaacgatgc ccaatcccct
    14881 ccgactggag cgttgagagg ctgacttgac gatctacgta tctgtgtgtg acgcccgaat
    14941 gaagaactgc agaacgtgcc cgttgaccaa atgatctttg acagagccac tcgggacttc
    15001 ccctatggct gttccaattt gtgagaggta tgctaaacat ctacgtgaaa tctgagaggg
    15061 tcccatgatt cgaatgccat ctcagaatga cgggatgtct ctgttgatcg tttcttgcgg
    15121 gctcattctc ctgtcagggg aacacagtct ggcggctgag agctcgccgc catctgcgga
    15181 taccaccatc cagcctcgag cattggaaga gtttaaaccg cttacgggtc ttgataacac
    15241 tcctcgaggc ggtactcttc ctggtggcac cttgccaggc gggacattgc ccagcggtac
    15301 tcttcctgat ggcactcttc caggaggcac tcttccagga ggcacgcttc cgggcggcac
    15361 attgcctggg ggaaccgtcc caggcggcac ggttcccggg ggaacgttac caggagggac
    15421 gttgcccgga ggcacgttgg aaggtggacc actttagtca cgtaccgttg gcgcctatac
    15481 gctaatcatg gcctgagggg agcagacgat gaaatccatc caactcgcac tgcgcgtgct
    15541 tgtaatctca gcccccgtgc tcttgctttc agcgccattg gggtgggctg atgaagacaa
    15601 aacaacacct cgaaaaggat ctgttcgggt cgaggatctg cc
//
LOCUS       DASSCQ010000218        18676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1907425, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000218 DASSCQ010000000
VERSION     DASSCQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 18676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18676
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907425"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="wecB"
                     /locus_tag="VFH05_13595"
     CDS             complement(<1..980)
                     /gene="wecB"
                     /locus_tag="VFH05_13595"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET6679304.1"
                     /translation="MKIVVIAGARPNFVKISPLLRAMRRRPAITPVLVHTGQHYDAQL
                     SDQFFTDLGIEAPAYNLEVGSGSHAVQTAEVMKRLEPVLGTEHPDRLMVVGDVNSTIA
                     AALVAVKLGIAVDHVEAGLRSFDRTMPEEINRILTDAIADQLFVSERSGVDNLLREGI
                     PAERIHLVGNVMIDALEACRPRWEQAPVLRRLGLEPTQPYALVTLHRPSNVDNPETLR
                     RIVDALETVSRELTLLWPVHPRTKQALQRDPRVQGRVTQEGNGATPHGLLALDPLGYL
                     DCLAVMSRARLVLTDSGGIQEETTVLGIPCLTLRSNTERPVTLTHGTNRL"
     gene            complement(977..2020)
                     /locus_tag="VFH05_13600"
     CDS             complement(977..2020)
                     /locus_tag="VFH05_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HET6679305.1"
                     /translation="MRIKPFVENILVLGQSHLATAITAEMKKRRRYRILDLMEPGEPG
                     RVFKDGVPFVGSLDRGLRTIPKTHRIVVALSERRGRLPIEALLDAKRQGVEVEDAVHL
                     YERLTGKLVLDQLQPSHLIFAPELGRGSLHSVWLRVISALVAAVGLVVCAPLFAVIAL
                     LIKWDSAGPVFFIQERVGQDGHKFPLLKFRTMRPVDRRPSEWVRDNGDRITAVGRWLR
                     KYRLDELPQLVNVLRGHMNLIGPRPHPASNYTLFMNRIPFYAFRSMVKPGITGWAQVC
                     YGYANSLEEETEKMCYDLYYIKHCSLSLDLNILIRTVKVILLGQQRVCHPEIQPVPQQ
                     PDKGTPDYSKEAA"
     gene            complement(2080..2913)
                     /locus_tag="VFH05_13605"
     CDS             complement(2080..2913)
                     /locus_tag="VFH05_13605"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET6679306.1"
                     /translation="MKEHTNPVLRTSPLESHPLSKSGRSDPRGEPVAHKIAARVKPDE
                     RLVSLVSPATIEAEQYRTLGLMLEQRRHSGHLQVVAVSSPTMGDGKTVTSINLAAALA
                     QSPQSRVLLVDADFRKPSVQAQLGMRDNNALGFRDAILNPDLALKDIVQRLPGTTLSV
                     LTVGRSQVMPHEVIKSTRFAEVLDEARRDYEWIILDTAPLVLAPDCLMMGRSVDGFVI
                     IVCAHKTSRKEIGEALDILGPSKLLGIVFNHDDSLLDSYGYRTYLSAAQAQQEELHQR
                     S"
     gene            complement(2945..4546)
                     /locus_tag="VFH05_13610"
     CDS             complement(2945..4546)
                     /locus_tag="VFH05_13610"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HET6679307.1"
                     /translation="MQDHDRRSIGVAIAEAWRRRKWLGLVCFSLPAAALLSLAMNLPN
                     LYSSTATVLVEQQHIPEGFVKSSVTGEADARLNLIREKLLNRAGLMALINRFNLYSEL
                     RKEAPEEAVVERMRKDVVLELKELQRQVYGRDNTFGFKVSYRGRNPETVATVANTLAA
                     QYVEENLSMRTNQATETTTLLKRQLNDIKQRHEEDERKIAAFKQRHMGELPEQVEVNL
                     STLGRLNQELTSNSEHQMRVLERRERLGKQLLDLNAGVPTTTVVNPDSMTHHLEKLRT
                     ELTELRTRASAKHPDVIRVQGEIAAIEKALAETAKDAKLDPAQSAALLSTPEDIKHAL
                     RELDHELKNLKADEQRLRAEAASIQRRVERSPQHAQEFQQLERDYKMTKDLYFSVLQR
                     YEDAQLAESMEQAKHVGQMRIVDPAMPSKAPVAPNRLKLILGALTLSLGLAAGCMILA
                     EQRDRSFHDVESLREFTKVPILVRIPEIVTKADRIRAYRRFTFKAAASMIGLVLIVGV
                     FSYIAWDNDQLVWMLSETKPAQQNR"
     gene            complement(4562..5206)
                     /locus_tag="VFH05_13615"
     CDS             complement(4562..5206)
                     /locus_tag="VFH05_13615"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET6679308.1"
                     /translation="MKTSRFIIIIGGVVALLSAAPCFAVDTPSSSKDSSVSNNSLSDQ
                     DHALAQAQEVGEYRIGPEDVLDISVWNNSAISRTVPVRPDGKISLPLVNDVQAAGLTP
                     NQLRSVLLKKLTEYVPSPEVSVIVREVHSFKVSVIGEVKKTGRHELKGRATVLDILAM
                     AEGFGEFAGRGRIVVLRPEGKTMRQIPFNYNKVMSNNGALENFFLQPGDIIVVP"
     gene            complement(5233..6483)
                     /locus_tag="VFH05_13620"
     CDS             complement(5233..6483)
                     /locus_tag="VFH05_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679309.1"
                     /translation="MRVLQWACCVLTMTMLVDPATVRAQLLQGIPRSPIETMGGSTER
                     MPEGVYLMPWLATGVVYDDNVFFSTRDRRQEDVFLRVTPGLQASYQSTRLTAIANYRF
                     DSEVYNKFSELSTVQQRQFGTVELRGRLSNNLNLNGIMGYAQTRTPYELNFLTSAQTA
                     RFETERFFINPSTEYRIDSLTKFRGEYAFSKDIFADEVNINSHIFNLGLERKVSAHDW
                     IGPAYVGRHFTFGGNLESPIAGFIGGNAEDVTSHAFLVSWAHDFTTDTRLDVRAGPRF
                     TNGSLDDRPEAYVGLRRRIPNGEVTLGYTSALTTIIGTVGPTRTDSVGVRVVYEPVKH
                     FTLTAAPSAAWVKSSSFQGTIYTAYVEAAYQFNKYVTAKGSAYFSYQESDFFTDGVVT
                     AENVVIPRNVYWLRLEFTYPSRWQ"
     gene            complement(6531..7124)
                     /locus_tag="VFH05_13625"
     CDS             complement(6531..7124)
                     /locus_tag="VFH05_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HET6679310.1"
                     /translation="MGNHGQTEGGGALSLLVNGNENRISEMIESGTRRWYALWTHSHC
                     EQLVRGQLDGRGFEVFLPTIGVWSSRAGVKHRIRRPLFPGYLFVRDELSGARYVELLK
                     ARGLVGVLGGQDGGPGEIPDTEIDAIRALVNSELPASPYPYLQTGQRVRITEGPLTGV
                     EGVLVDRRPGQSLLVLSVHLFRRSIAVEVDCALAIPI"
     gene            7497..8615
                     /locus_tag="VFH05_13630"
     CDS             7497..8615
                     /locus_tag="VFH05_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6679311.1"
                     /translation="MKIAQLSPLWESVPPQLYGGTERIVSYLTEELVRQGHEVTLFAS
                     GDSKTAAHLKACCDRALRLHDNGMINRDAPNILMQEWALGAESGHYDVVHSHLDFLSF
                     PMSRRAHAPVVTTLHGRLDLPELQPIFNEYSELPVVSISNAQRKPLPGANWAGTVYHG
                     IPKDLYSLHERPGSYLAFLGRISPEKCPDHAIEVAKRVGMPLRIAAKVDAADHAYFKQ
                     KIERLLDHPLVEYVGEINDAEKNDFLGEAAALLCPYDWPEPFGLVLIEAMACGTPVLA
                     YRQGSIPEVIDHGVTGYICDNIAEMAQAVAGLPLIDRQRCRDVFEKRFTVERMAKDYL
                     NLYESMVAAPHRPRLLTRPASHARVGAIHTLTPQADVA"
     gene            complement(8672..9808)
                     /locus_tag="VFH05_13635"
     CDS             complement(8672..9808)
                     /locus_tag="VFH05_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HET6679312.1"
                     /translation="MGRTANWRRKGGRRCTNRLRPDPDRLIGEIDDEGREWLMKVAFN
                     GLGRIGRAAFKILATNPELDVVAINDVHPPENLAYLLNHDTVYGRYPNRVTVRNGALM
                     MADRSFPTFMERDPARLPWGDLQVDIVFECTGQFNQRQDLSKHLDAGAKRVILSAPSM
                     DDDIPTVIFGVNVYDEKHGAILSCGSCTTNCVAPIVEIIGRRIGLRKAVMNTVHAYTA
                     SQGIVDGSKKNMRKGRAGAANLVPSTSGAATATARALPSLRGRFDATAVRAPVPICSI
                     ADVVMVTARTTSVEEVNTLFLEEARSERYEGVVGVTEDPVVSTDIIQDPRASIIDREM
                     TQVVDGDLVKVMSWYDNEWGYASQMVRQAVSMAQIVPVEPAFSR"
     gene            complement(9744..10877)
                     /gene="tal"
                     /locus_tag="VFH05_13640"
     CDS             complement(9744..10877)
                     /gene="tal"
                     /locus_tag="VFH05_13640"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET6679313.1"
                     /translation="MSRLLELYTCGQSYWLDNLSRGMIRSGDLKRRIGEEGLRGVTAN
                     PSTFALALKSGDYNADIQNLARQGHSTEQIYERLLVGDVQEACDLFMDMNHASDRLDG
                     YVSLEVSPHLAHDATGTMREARRFHELVDRPNLLVKVPGTMAGFLAIEQLLYEGIPIN
                     VTLLFSLPAYEAVIEAYLSALERRSAERRSLKEVTSVASFFLSRIDRLVDQLLRQLDR
                     PSEGRPMSAALRGKAAVASAKLAYRSFRRAFSGERWDRLAVKGARLQRPLWASTGTKD
                     PSYHDLMYVEPLIGPDTVNTMPEQTIRAFADHGQVKPDQIAQGVEEAARTIQNLSEAG
                     IDLRCVAWQLEHEGIRQFTEAFDGAHRELEEKRRQAVHQPAAA"
     gene            complement(11050..13698)
                     /gene="ligD"
                     /locus_tag="VFH05_13645"
     CDS             complement(11050..13698)
                     /gene="ligD"
                     /locus_tag="VFH05_13645"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET6679314.1"
                     /translation="MKRATVASQLQRYRRKRNFVKTPEPAGHPGAIRQTPAPLYVVQK
                     HAATRLHYDFRLELDGTLKSWAVPKGPSLDPSQKRLAVHVEDHPLEYAKFEGVIPPKQ
                     YGAGTVLIWDRGTWQPQGDPRDGYRKGVLKFRLDGRKLHGAWTLVRMHGRRDEGGHSD
                     KENWLLIKEQDADARKGAAGEIVDSSTKSVESGRDLKDIAGKGARVWQSNRSARASVA
                     QASVHSSRAPSSSRPVLSAQDFSSVTGAVKARQPDWIAPQLATLVDRMPTDEGWLHEI
                     KFDGYRLLCRVRNGEARLFTRNGNDWTSKLQTQADAIVRLGMKDAWLDGEAVVFTEDG
                     KSSFQALQNAFDSRFTGTIVYCVFDLLYANGYDLRQTPLMERKRLLASLLNGRRIDSV
                     LKYSDHIIGDNQASFEEACRQGLEGLIVKRMDSTYKAGRGRSWIKVKCEQRQEFVIGG
                     FTEPTGSRAGFGALLIGYYENGQLYYAGKVGTGFTDSLLKKLHRMLGELEQPDPPFAN
                     PPTGYEAKGAHWVTPKLVAEVRFAEWTQEGILRQASFQGLRTDKPAKAIGRERPQQSP
                     GEGPPVPVDKENGSQETSRKRSLKGLRLTNPDKVLYPDIGLTKAGLVRYYEQIGDWML
                     PHVKGRPLTLVRCPEGYQKCFYQKHINEKMPPAVGRIEIEEEKGRDTYMLAESLEALL
                     GLVQMGVLEIHTWGSLKDKVERPDRLIFDLDPDPSVPWRQVIEAAQLTKALLEELGFV
                     TFLKTTGGKGLHIVTPIQRTVEWEEAKGFAKMVADHLVSTIPQRFTSNMSKRARKEKI
                     FIDYLRNARGATAIAAFSTRAKPGAPVSVPIAWEELSETLPSDHFTVANLPARLEHLR
                     QDPWNTYAQSARRVTADMKARLKSGL"
     gene            complement(13705..14439)
                     /locus_tag="VFH05_13650"
     CDS             complement(13705..14439)
                     /locus_tag="VFH05_13650"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HET6679315.1"
                     /translation="MNRTQVIVALFVCIVMLGSGCAKSAHQTGEDQLQRLIPSSVLSQ
                     VDETVSFSDLLAEPGNYVGRTVMFSGLALKSRRAKDSTEIEVLQLPTDRGMSPSDRKA
                     RSEGRFLAVQSDGFLDPAVIEKDSPVTVVGEVKGATTKPLDEGEYQYPVLDIKQLVDW
                     NEVRTQERDTYYDSPYAGRYGGYYGPWSSWYYGFGSPFAGPYGFYPYSYYGPYYGPYY
                     GFPRGYSVPAPAPPPPSSIPPQFRKD"
     gene            14859..16220
                     /locus_tag="VFH05_13655"
     CDS             14859..16220
                     /locus_tag="VFH05_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679316.1"
                     /translation="MRLLGRFFIFILLSTYAVAFLTSCAGEDAGSSSALGTFTAAGGF
                     IEHVPGDLRPRWSAAELRTFLPTTRGKFMFPAPYNTEAFRITDASDCGGTDCVTPVGY
                     SYWRNSNAHEGSNDMWIFLSLNSQKGGSGPTLFRLDKATDSITKVGPLFPTTSKFSGR
                     SGDGWYFSASRANQLYINDGPKLLRYDVTTHTFETVFDLTGEFGSDREVWQMHSSNDD
                     LVHSATLRAKGAGTFLGCLVFTETTGQFSYFAKKGTYDECHIDKSGHYLMILENIDGR
                     NGSENVIVDLQTGIETVIHDEKGGVGHADMGYNYVVGADGYSPLPNAFITWSFAPSLV
                     KGPTVFHSSSSKIPRVNHVSHQNAKPGLPMHQQFACGSSADRGAAQNEILCFLLDGTE
                     RELVVAPVMTDLDASGGGSEYYKLPKGNLDITGQYFIWTTNTGGNRIDAFLVKVPAQL
                     LLS"
     gene            complement(16330..16914)
                     /locus_tag="VFH05_13660"
     CDS             complement(16330..16914)
                     /locus_tag="VFH05_13660"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HET6679317.1"
                     /translation="MSTVKRLMLFRPLVACGLLCTATACSGPQVIPSELEEQIDSSVS
                     FRDIQASPQSLQGKTVLLGGEVLAAKRVQEGTELEILQLPVTTDDPPAERRSESQGRF
                     LALDRTLTDPASLPPGTRLTLVGAVTGEEIRRLDESTYRYPTVEAKHMYVWEPDVYRE
                     RSRRPVGLFGGVGFGFGSGGRSGSFGGVGIGTGF"
     gene            complement(16928..17557)
                     /locus_tag="VFH05_13665"
     CDS             complement(16928..17557)
                     /locus_tag="VFH05_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6679318.1"
                     /translation="MRARAAWCVLLAGIACSVSLGGRAQAEYYVAGQIGVNFADRLKN
                     IEGTGSLLNLRAPDFDLKNAVSYGGKLGYFPGNGPTGVELDVFNSQPNIKNLDDVPGI
                     HLGVTNVGVNLLMRYPGQTFQPYVGIGGALLIARLSESATTRSDTDVGFGLNVLAGVR
                     AFITPYVAIFTEYKYTHGTLTFDDAFGPVGGFSGDYQVQNVMFGVSYHF"
     gene            complement(17687..>18676)
                     /locus_tag="VFH05_13670"
     CDS             complement(17687..>18676)
                     /locus_tag="VFH05_13670"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6679319.1"
                     /translation="LYAMGLLSLPVLYVLVFVIAMFSMAFGPALNATLPLIVKKDQLT
                     RINALMQSAMTIGQLLGPALSGILIAAIGAQNALYVNAGAFLISALCKLPLRLPDIRP
                     HRTSQNVWQQAVNDLQDGIRFVFVKQRLLFLLMIVASIFTLGSTAFVYLLPVIGDRLL
                     HVGAVELGWLWSSLSVGILITTAWLLWRPQQSFCARIWMIAVAAGVAAFAVPALLISE
                     SVLVAAGLIAGIGATSGLITPLVSASLQERTPKDLMARVFGLFNTGTMVFSMIGITLF
                     GWLADSFGPGVSLTTIGIVNGAAAVVSFVLLPWCYRLAKETSVRSEQSLRRAS"
BASE COUNT         3878 a         5570 c         5250 g         3978 t
ORIGIN      
        1 atcagccggt tggtgccgtg ggtcaaggtc actggtcgct cggtattgct gcgcaaggtc
       61 agacacggaa tgcccaacac tgtcgtttcc tcttgaatac cccctgaatc cgttaggacg
      121 agtcgagccc ggctcatcac ggcgagacaa tcgaggtatc cgagcggatc aagcgccagc
      181 aatccatgag gagtcgcgcc gttcccttcc tgggtgacac gcccctgcac gcgagggtct
      241 ctctgcaacg cctgtttcgt ccgcggatgg acgggccaca agagtgtgag ctcgcgggag
      301 acggtctcga gcgcgtccac aatccgtctg agcgtctcgg gattgtccac gttcgaggga
      361 cgatgcaggg tgaccagcgc atacggctgc gtcggctcaa gcccgagacg ccgcaagacg
      421 ggggcctgtt cccagcgagg ccggcaggct tccagagcat cgatcatgac gttgcccacc
      481 aagtgaatgc gctcggcggg aatcccctcc cgcagcagat tgtccacccc gctgcgttcg
      541 ctgacgaaca actggtctgc gatcgcgtcg gtcaaaatgc gattgatttc ttccggcatc
      601 gtacgatcga agctgcgaag ccccgcttcc acatgatcca ctgcgatgcc aagtttgacc
      661 gccacgagag ccgcagcgat ggtgctgttg acgtcgccca ccaccatgag gcggtccgga
      721 tgttcggtgc cgagcacagg ttccaggcgc ttcatgactt cagcggtctg gaccgcgtgc
      781 gaccccgatc ccacctccaa attgtaggct ggcgcctcga tcccgaggtc tgtaaaaaac
      841 tgatcgctga gctgcgcatc gtaatgttgg ccggtatgca cgagcacagg tgtgatagct
      901 gggcggcgac gcatcgcccg cagcagcgga ctgattttga caaaattggg gcgtgccccg
      961 gcaatgacaa cgatcttcat gccgcttcct tggaatagtc cggagtgccc ttgtcaggct
     1021 gttgagggac cggctggatc tcgggatggc agactcgctg ctgacccagc aggatgactt
     1081 tgaccgtccg gatcagaata ttcagatcca gactcaggga gcaatgcttg atgtaataca
     1141 aatcgtagca catcttctcc gtttcctctt cgagactgtt ggcatatcca tagcaaacct
     1201 gggcccaacc cgtgatgcct ggcttgacca tcgaacggaa cgcataaaat ggaatgcgat
     1261 tcatgaagag cgtgtaattg gaagccggat gcggtcgagg gccgatgaga ttcatgtgtc
     1321 ctcgtagcac attgacaagc tgcggcaatt catccagccg gtatttgcgt agccaccgtc
     1381 cgacggcggt aatacggtcg ccgttatccc ggacccattc cgatggacgc cggtcgaccg
     1441 ggcgcatggt acggaacttc aggagaggaa acttgtgtcc atcctgccca actcgctcct
     1501 ggatgaaaaa cacgggaccg gccgaatccc atttgataag cagggcgatg accgcaaaaa
     1561 gcggagcgca aacgaccaag ccgaccgctg ccaccagggc gctgatcacc cgcagccaca
     1621 cggaatggag ggagccgcgc cccagttcgg gagcaaagat caaatgactg ggctgcaact
     1681 gatcaaggac gagtttgccc gtcaatcgtt catacaaatg aaccgcgtcc tcgacctcca
     1741 ccccttgtcg cttcgcatcg agtagcgctt caatgggcaa ccgtccccgg cgctcgctca
     1801 aggcgacgac gatccggtgt gtcttcggaa tcgtcctcag cccccgatca agcgatccga
     1861 cgaaggggac gccgtctttg aatacgcgtc ccggctctcc cggttccatc agatccagga
     1921 tgcggtatcg ccggcgcttt ttcatttcag cggtgatcgc ggtcgcgaga tggctctgtc
     1981 cgagaacgag gatattttcg acaaaaggct ttatgcgcat ggtgttgtac tctggttcag
     2041 ggtggtgatg acgcttgtcg cttccgatct gcctccgact taagaacgtt gatgtaactc
     2101 ctcctgttga gcctgagcag cggagagata ggttcgatac ccatatgagt cgagcaggct
     2161 gtcgtcgtgg ttgaacacga tgccgagcag cttggatggg cccaggatgt ccaacgcttc
     2221 accgatttcc tttcgtgacg tcttatgcgc gcagacgatg atcacgaatc catccacgct
     2281 gcgccccatc atcaagcaat ccggagccag cacgaggggg gcggtatcca gaatgatcca
     2341 ttcgtagtcc cggcgcgctt catccagcac ctcggcgaaa cgcgtcgatt taatcacctc
     2401 gtgcggcatg acctgggatc gaccgaccgt cagcaccgac agcgtcgtcc ccggaagtcg
     2461 ctgcacgatg tctttgaggg caaggtcggg attcagaatc gcgtcacgga acccgagcgc
     2521 attgttgtct cgcatgccca gttgcgcctg caccgagggt tttcgaaagt cggcatcgac
     2581 gaggaggaca cgggactgag gagattgagc cagggcggcc gccagattga tggaggtgac
     2641 cgtcttgccg tcacccatcg tcgggctcga caccgccacc acctggagat gcccggaatg
     2701 gcggcgttgc tcgagcatca atccgagcgt tcgatattgc tctgcctcga tcgtggcggg
     2761 ggagacaagg ctcaccagtc gctcgtccgg cttgacccgt gcggcgattt tgtgcgccac
     2821 cggttcccca cgtgggtcgc tccggccgga tttggacaag gggtgggatt ccaaaggcga
     2881 cgtccgtaag acgggattcg tgtgttcttt catgtcgtca atccttcaac aatgaacgat
     2941 tgtcctaccg gttttgctgt gcgggtttgg tttcactgag catccacacc agttgatcgt
     3001 tgtcccaggc gatgtacgaa aacacgccga cgatcagcac taacccgatc atcgaggccg
     3061 ccgccttgaa tgtgaatcgc cgataggctc tgatccggtc ggcttttgtg acgatttcag
     3121 gaatgcgtac gagaatgggg acttttgtaa actcacgcaa gctttccaca tcatggaagg
     3181 accgatcccg ctgttcggcc aggatcatgc accccgcagc caggcccaac gacaatgtca
     3241 gcgcgccaag aatcagtttg agccggttgg gcgctaccgg cgctttcgaa ggcatggcag
     3301 gatccacgat gcgcatctgg ccgacatgct tggcctgttc catcgattcg gcaagctgcg
     3361 cgtcttcgta gcgctggagc accgagaaat acagatcctt cgtcatcttg taatcgcgct
     3421 ccagttgctg gaactcctgc gcgtgctgtg gagaccgttc gacccgccgc tgaatcgagg
     3481 cggcttccgc gcgcaaccgc tgctcatccg ctttcagatt tttcagctcg tgatcaagtt
     3541 cgcgcaaggc gtgtttgatg tcctccggag tggacaatag cgccgcggat tgagcagggt
     3601 ctaacttcgc gtctttggcg gtctccgcca gagccttttc gatcgccgcg atctcgccct
     3661 ggacccgaat gacatccgga tgcttggcgc ttgctctggt acgtaattcg gtgagctccg
     3721 tgcgaagctt ttccaggtga tgcgtcatgg aatcagggtt gacgactgtc gtggtgggga
     3781 cacccgcatt caaatcgagc agctgcttcc ccagccgttc ccttcgctcg agcacacgca
     3841 tttgatgctc gctgtttgac gtcagctcct ggttcaggcg tccaagcgtt gagaggttga
     3901 cttcgacctg ctcgggcaat tcccccatgt ggcgctgctt gaaggccgcg atcttccgct
     3961 cgtcctcttc atgccgttgc ttgatatcgt tgagctgcct tttcagcagg gtcgtggttt
     4021 ccgtcgcctg attggtgcgc atgctcagat tttcttccac gtattgggcg gccagagtat
     4081 tggcgacggt cgcgacggtc tccggattgc ggccgcgata actcactttg aagccgaagg
     4141 tgttgtcccg gccgtagacc tgccgctgca attctttcaa ctcaagcacg acgtctttgc
     4201 gcatgcgttc gacgacagct tcttcggggg cctcctttcg caattccgaa tagagattga
     4261 atcgattgat cagcgccatc aagccggcgc ggttcagcaa tttttcacgg atcaggttga
     4321 ggcgcgcgtc ggcttctccg gtcacggaag acttcacaaa gccttccgga atatgctgct
     4381 gctcgaccag cacagtggcc gttgaactgt agagattggg caaattcatc gcgaggctca
     4441 gcaaggcggc ggcagggagc gagaagcaga caagcccaag ccattttctg cgccgccatg
     4501 cctcggctat cgcgacaccg atcgatcgtc tatcgtggtc ttgcatgatc gctccacacg
     4561 gttacgggac gacgatgatg tcacccggtt gtaggaagaa attttccagc gctccgttgt
     4621 tggacatgac cttgttgtag ttgaatggaa tttggcgcat cgtcttgcct tcaggtcgca
     4681 ggacgacaat acgccctcgt ccggcgaatt ccccgaaccc ttcggccatc gcgagaatgt
     4741 ctagcacggt cgctcgtccc tttaattcat gcctcccagt tttcttcacc tcaccgatga
     4801 ctgaaacttt aaagctgtgc acctctctga cgatgaccga gacttccggc gagggaacat
     4861 attccgtcag ctttttcaga agcacgctcc gcagttggtt gggggtgagt ccggcggctt
     4921 gaacatcatt gaccagcggc agcgagatct tgccgtccgg ccggacagga acggtccggc
     4981 tgattgcact gttgttccag acagaaatgt ccaggacatc ctccggtccg atccgatatt
     5041 cgccgacttc ctgcgcctgc gctaaggcgt gatcctggtc cgacaggctg ttattcgata
     5101 cgctcgagtc cttcgaggaa gacggcgtat ccactgcaaa acagggcgcc gcgctcagta
     5161 gcgcaaccac accaccgatg atgatgataa atcgtgacgt cttcatataa acctccggtc
     5221 ccctatggac ggttactgcc accgtgacgg atacgtgaat tcgagccgca accaatacac
     5281 attgcgaggg atgacgacat tctcggccgt aacgacccca tccgtgaaga agtcgctttc
     5341 ctggtaactg aaataagccg atcctttggc tgtcacatac ttattgaatt gataggccgc
     5401 ctcgacatag gcggtataaa tggtgccctg aaacgaactg ctttttaccc aggcagcact
     5461 tggtgccgcg gtcagagtga aatgcttgac gggttcgtag acgacccgta ctcccacgct
     5521 gtccgtccgt gtgggcccga cggtgccgat gatcgtagtg agtgcactcg tatagccgag
     5581 ggtcacctcg ccgtttggga tgcggcgccg gaggcccaca taggcttccg gccggtcatc
     5641 cagcgacccg ttcgtgaaac ggggtcccgc ccgaacgtca agccgcgtgt ccgtcgtaaa
     5701 atcatgggcc caactcacca gaaaggcgtg tgaggtgacg tcctcggcat tccctcctat
     5761 gaatcctgcg atcggacttt cgagattccc gccaaacgta aagtggcgcc cgacatatgc
     5821 cggcccgatc caatcgtgcg cgctcacctt ccgctcgagg ccgagattga aaatgtggga
     5881 attaatgttc acctcgtcag cgaagatgtc cttggaaaaa gcgtattcgc cgcgaaattt
     5941 ggtcaacgaa tcgatgcggt attcggtgct tggattgatg aagaatcgct cggtctcaaa
     6001 acgggccgtc tgggctgacg tgagaaaatt caattcgtat ggtgtgcggg tttgcgcgta
     6061 tcccatgatg ccgttgagat tgaggttgtt cgagagacgt ccccgaagtt caacggtgcc
     6121 gaattgccgc tgctgtaccg tcgaaagctc gctgaacttg ttgtacacct ctgagtcaaa
     6181 ccggtaattg gcgatggcgg tcaggcgcgt cgattgatag ctggcctgca gaccgggggt
     6241 gacacggaga aagacatcct cctgtcgacg gtccctcgtg gaaaaaaaca cgttgtcatc
     6301 gtacacgacg cccgtcgcca gccatggcat caggtaaacg ccctcaggca tgcgctcggt
     6361 ggaaccgccc atggtttcga tgggagaacg cggaatccct tgtagcagtt gggccctgac
     6421 cgttgctgga tcaacaagca tcgtcatcgt caagacgcaa caggcccatt gcagaacacg
     6481 catcgcatcg cccttacggc ctcgtgcgaa gccacgccgt atgccgggag tcatatcgga
     6541 attgccaatg cacaatcgac ctcgacggcg atgcttcggc ggaagagatg cacggacaac
     6601 accaagaggc tttgtccggg acgccggtcc accaacacac cttcgacgcc ggtgagcggc
     6661 ccttcggtga tgcgcacccg ttggccggtc tgtaggtacg gatacggtga tgccggcagc
     6721 tcggagttca caagcgcgcg gatcgcatcg atctccgtat cgggaatctc tcccggccct
     6781 ccgtcttgtc cgcccagcac acctaccagt cccctcgcct tgagcagctc gacgtagcgg
     6841 gcaccgctca actcgtcccg cacgaacaga taaccgggaa acaaggggcg gcgaatcctg
     6901 tgcttgacac ccgcacgaga agaccacacg ccgatggtgg gaagaaagac ttcgaaacct
     6961 cggccatcga gctgtcctct caccaattgt tcgcaatggc tgtgggtcca aagggcatac
     7021 cagcgtcgag ttccgctctc gatcatttcc gaaatccgat tctcgttgcc gttcacgaga
     7081 agtgacaagg ctccgccccc ttcggtttgg ccatggttcc ccatcccgaa tctccattgc
     7141 ctttcttccg gcagccgacg atacttattt gcagaggccg tcgtctgcgc ttcagagaac
     7201 acgctcagcg gttccctagc accgccggtt tgtatcccct gttctggacc accgtaacga
     7261 atacaggagg tggggactat ccgtgatgtc actgaaactg ctggggagaa tacctgaggg
     7321 tgtatcggta gtattgtgga gggcccgtca aggggcctac tagtctctgc cctggggtgc
     7381 cgaaccgggt actctctaga agtttcccta gttcgagtgg cgcctactct tcgccatagt
     7441 aaagcaactc accattcata ttcgctcgaa tctctatttt cacagaaagg actgccatga
     7501 agatcgctca gttgtctccg ctctgggaaa gcgtcccgcc tcaattgtat ggcgggaccg
     7561 aacgaatcgt ctcctacctg accgaggagc tcgttcgtca aggtcacgag gtgacgctct
     7621 ttgcgagcgg ggactcgaag actgcagcgc atctcaaagc ctgttgcgat cgtgcactgc
     7681 gtctccacga caatgggatg atcaatcgcg acgcgcccaa tatcctgatg caggaatggg
     7741 cgctcggcgc tgagtcagga cactatgacg tggtgcattc gcatctggat ttcctgtcgt
     7801 tcccaatgag ccggcgcgct catgcacccg tggtgaccac gctgcatggt cgcttggact
     7861 tgccagagtt acagcccatc ttcaacgagt attcggagct gccggtggtc tccatttcaa
     7921 atgctcagcg aaaacccttg ccaggcgcaa actgggcagg gaccgtctat cacgggatcc
     7981 ccaaggattt atattcgtta catgagcgcc ctggatcgta tctggcgttt ctcggtcgga
     8041 tttcgccgga gaaatgcccg gatcatgcga ttgaggtggc caagcgggtc ggcatgcccc
     8101 tccgcatcgc cgcaaaagtc gatgcagcgg accacgccta tttcaaacag aagatcgagc
     8161 gtctgctcga ccatccctta gtcgaatacg tcggtgagat caacgatgcc gaaaaaaacg
     8221 attttctggg cgaagcagcc gcacttctct gcccctacga ctggcccgaa ccgttcggac
     8281 tcgtgttgat cgaagcgatg gcttgcggca cgccggtatt agcctatcgg cagggatcga
     8341 ttcctgaggt catcgatcat ggcgtcacag gctatatctg cgacaacatc gcggaaatgg
     8401 ctcaagcggt tgcgggactt ccgctcattg accgacaacg atgccgcgac gtcttcgaaa
     8461 agcggtttac cgtcgagcga atggcgaagg actatctcaa tctctatgag tcgatggtgg
     8521 ctgctcctca tcgaccccgg ctcctgacgc gacccgcgag ccatgcgcgt gtcggtgcca
     8581 ttcacacact gaccccacaa gctgacgtag cgtagctgat gaatgctgag tctccttcgg
     8641 gagactcagc actccccgag tattcctttg tttaacggga gaaggcgggt tcgacaggaa
     8701 cgatttgggc catgctgacg gcctgccgca ccatttggct ggcgtaaccc cactcattgt
     8761 cgtaccaact catcaccttc acgagatcgc cgtcgacgac ctgtgtcatt tcgcggtcga
     8821 taatagaggc ccgcgggtct tggatgatgt cggtagagac caccgggtcc tccgtgacgc
     8881 ccacgacacc ttcataacgc tcactcctcg cctcttcgag aaacagcgtg ttgacttcct
     8941 cgaccgaagt ggtgcgtgct gtcaccatga ccacatccgc aatgctgcag atggggaccg
     9001 gcgctcggac tgccgtcgca tcgaatcgac ctcgcaatga gggcaaggcc cgtgcagtcg
     9061 ccgtagccgc tccgctggtc gagggcacaa ggttcgccgc accggcacgg cccttccgca
     9121 tgttcttctt cgagccgtcg acgatcccct ggctcgcggt atacgcatgg acggtattca
     9181 tgacggcttt gcggagtccg attcgccgcc cgatgatttc cacaatcggg gcgacgcagt
     9241 tggtcgtgca actgccacac gagaggatgg cgccgtgttt ttcatcatag acgttgacgc
     9301 caaaaatgac cgtggggata tcgtcgtcca tggacggcgc cgacaggatg acgcgtttcg
     9361 cgccggcatc caagtgcttg gagaggtcct gccgctgatt gaattgcccc gtgcattcga
     9421 acacgatatc gacttggagg tcgccccatg gaagccgcgc cgggtctcgt tccatgaacg
     9481 tcggaaagga ccggtccgcc atcatcaacg cgccgtttcg aaccgtgacc cgattcggat
     9541 agcggccgta gacggtgtca tgattcaaca ggtaagccag gttctcggga ggatgcacat
     9601 cattgattgc gaccacatcg agttccggat tcgtcgccaa gattttgaag gccgcccgtc
     9661 cgatccgtcc caaaccattg aaggcaactt tcatcaacca ctccctcccc tcatcatcga
     9721 tctccccgat gagccgatcg ggatcaggcc gcagccggtt ggtgcaccgc ctgccgcctt
     9781 ttctcctcca gttcgcggtg cgccccatcg aatgcttccg taaattgacg gatcccttca
     9841 tgctcgagtt gccatgccac acagcgcaga tcgatgcccg cttccgatag gttctgaatc
     9901 gtccgagccg cctcctcaac cccttgcgca atttgatccg gcttcacctg tccatggtct
     9961 gcaaaggccc gaatagtttg ttccggcatc gtgttcacgg tgtcgggccc gataaggggc
    10021 tccacataca tgaggtcatg gtacgagggg tctttggtgc ccgtactggc ccagagaggt
    10081 cgttgcagcc ttgctccctt caccgccaat cggtcccatc gttcgccgct gaacgcccga
    10141 cggaatgatc ggtacgcaag tttggcagag gccacggccg cctttcctcg cagcgccgca
    10201 gacatcggac gcccttcgct cggccggtcc aattgtcgaa gcagttggtc gacaagtcgg
    10261 tcaatgcggc tgaggaagaa gctcgccacc gatgtgacct ccttcaacga tcgtcgttca
    10321 gccgatcgac gttccaatgc ggacaggtac gcctcgatca ccgcttcata ggcgggcaac
    10381 gaaaacagca acgtcacatt gatgggaatc ccttcataga gcagctgctc gatagcgagg
    10441 aacccggcca tcgtccctgg aactttgaca agcaagtttg gacgatcgac cagctcgtgg
    10501 aatcggcggg cttcgcgcat ggtgccggtc gcatcgtgcg caaggtgcgg ggacacttcg
    10561 agactcacat atccatcgag gcgatccgag gcgtgattca tgtccatgaa cagatcacag
    10621 gcttcctgaa cgtccccgac gaggagccgc tcataaatct gctcggtgct atggccttgt
    10681 cgagcaaggt tctggatgtc ggcattgtag tcaccgcttt tcagagcaag ggcaaacgtg
    10741 gacggattgg ccgtgacgcc gcgcaaacct tcctcgccga tccggcgttt cagatctcca
    10801 ctgcggatca tgccgcgact gagattatcc agccaatagc tctgaccgca tgtgtaaagc
    10861 tccagcaagc gtgacatcaa tccctccggc aagtgttgat tctggtttta agaatcggcg
    10921 ctgtacactc agatgctccc tagccgccgt ctttctgtct tgattgaatc ctatgcgcgc
    10981 gaacgatcat cgaagcggat ggtcgtacag tgatcggacg ctgagcgccc aaagaggcca
    11041 tagccaacct cagagcccgg atttgagccg tgctttcatg tcagccgtca cacgtcgggc
    11101 cgattgggcg tatgtgttcc atggatcttg ccgaagatgc tcgaggcggg ccggcaggtt
    11161 ggccaccgta aaatgatcgg atggcagcgt ctcggagagc tcttcccagg caatgggcac
    11221 agaaaccggt gcgcccggct tcgccctggt cgagaatgcg gcaatcgccg tcgctccccg
    11281 agcattgcga aggtaatcga taaatatttt ttctttccgg gcacgtttcg acatgttcga
    11341 ggtgaaccgc tggggaatgg tcgaaaccag atgatcggct accattttcg caaagccctt
    11401 cgcctcctcc cactccacgg tgcgctgaat gggggtgacg atgtgcagtc ccttgccgcc
    11461 cgtcgttttg aggaacgtga cgaacccgag ttcctccaaa agggctttgg tcaactgggc
    11521 tgcttcaatc acttgtctcc acggcaccga agggtcaggg tcaagatcga agatcaggcg
    11581 atcgggacgt tccaccttat ccttgagaga accccacgta tggatctcca agacgcccat
    11641 ctgcaccagg cccaacagcg cctccagcga ctcggcaagc atgtatgtgt cgcggccctt
    11701 ctcctcctcg atctcgatgc ggccaacggc cggaggcatc ttttcgttga tatgcttttg
    11761 gtaaaagcat ttttggtatc cctccgggca acggaccagc gtcagcgggc ggcccttcac
    11821 gtgggggagc atccagtccc cgatctgttc gtaataccgg acgagtcctg ctttggtgag
    11881 accgatgtca ggatacaaga ccttatcggg attcgtcagt cgtaatccct ttagggaccg
    11941 cttgcggctc gtctcctgac tcccgttctc cttgtcgacc gggacaggag gcccctcccc
    12001 tggagattgc tgcgggcgtt cccgtccgat ggctttggcc ggcttgtccg tcctgagacc
    12061 ctggaaactc gcctgccgca gaatgccctc ctgagtccat tctgcgaacc ggacttcagc
    12121 gaccagcttc ggagtaaccc agtgagcgcc tttcgcctcg taccccgtcg gcggattggc
    12181 gaacggtgga tcaggctgtt caagctcacc cagcatgcga tggagctttt tcagcaggct
    12241 atcggtaaaa ccggtgccga ccttgccggc atagtataac tggccgttct cgtaatagcc
    12301 gatgagaagc gcgccgaatc ctgcccgtga tccggtaggc tcagtaaacc ctccgatcac
    12361 aaattcctgc cgctgttcgc acttcacttt gatccagcta cgaccccggc ccgccttgta
    12421 ggtggaatcc atccgtttga cgatcaaccc ttcgagcccc tgccggcacg cttcctcaaa
    12481 cgaggcctgg ttatctccaa tgatgtgatc gctatacttg agcacggaat cgatccgtcg
    12541 accgttcaag agtgaagcca ggaggcgttt ccgctccatg agtggagtct ggcgcaagtc
    12601 atatccgttc gcatacagca ggtcgaagac gcagtacaca atggtacccg tgaatcggga
    12661 atcgaaggca ttctgtaagg cctggaagct ggattttcca tcctcggtaa agacgactgc
    12721 ttcgccatcg agccaggcgt ccttcattcc caaacggacg atggcatcgg cctgagtctg
    12781 cagcttgctg gtccaatcat tcccattgcg ggtgaacagg cgcgcctcac cgtttctcac
    12841 tcgacagagc aggcgatacc cgtcaaattt gatttcatgc agccacccct catccgtcgg
    12901 catgcggtca accaacgtcg ccaattgcgg cgcgatccaa tccggctggc gcgccttgac
    12961 ggccccggtt accgacgaaa agtcctgagc gctcagcacc ggacgcgaag aggatggcgc
    13021 gcgcgagctg tgaacggacg cttgagcgac ggaagcacgt gcacttcggt tagactgcca
    13081 gacgcgagca cccttcccgg cgatgtcttt cagatctcgc ccgctctcga cacttttcgt
    13141 cgagctgtcc acgatttcac cggctgcgcc ttttcgagca tccgcatcct gttctttgat
    13201 caacagccaa ttctccttgt cactatggcc cccctcatcc cgtcgaccat gcatgcgcac
    13261 gagtgtccac gcgccgtgaa gttttcggcc gtccaatcga aacttcagca ctcccttgcg
    13321 ataaccgtct cgcggatcgc cctgcggttg ccaggtgccc cgatcccaga tcagcaccgt
    13381 ccctgccccg tattgtttcg gcggaatcac gccctcgaac ttggcatatt ccagcggatg
    13441 atcttcgacg tgaaccgcca gccgcttctg ggacggatcg aggctggggc ctttgggaac
    13501 cgcccaactt ttcagtgtgc cgtcgagttc cagccggaaa tcatagtgta gacgtgtggc
    13561 ggcatgtttc tgcacgacgt acaggggggc tggggtctga cggatcgcgc cgggatgccc
    13621 ggcgggttcc ggcgtcttca caaaattgcg tttgcggcga tagcgttgga gctggctggc
    13681 aaccgtcgca cgcttcatgg gccgctagtc ttttctgaat tggggtggaa tggacgacgg
    13741 aggaggagga gcgggagccg gaaccgaata gccgcgggga aacccataat agggtccgta
    13801 atagggccca tagtaggaat agggatagaa gccataaggc cccgcgaaag gactgccaaa
    13861 gccgtaatac cacgatgacc agggcccgta gtatccgcca tatcgcccag cgtagggcga
    13921 gtcgtagtac gtgtcgcgct cctgagttct cacctcgttc cagtcgacca gctgcttgat
    13981 gtcgagaacc ggatattgat actcgccttc gtcaagcggt ttggtcgtcg cacccttgac
    14041 ctctcccacc acggtcaccg gcgagtcctt ttcaatgacg gcgggatcta aaaatccgtc
    14101 cgactgcacc gccagaaagc gtccctccga ccgtgccttt cgatccgatg gcgacatgcc
    14161 gcgatccgtc ggcagttgga gaacctcaat ctccgtgctg tcctttgccc gccgcgactt
    14221 cagggccaac ccgctgaaca tcaccgtacg tcccacatag ttaccgggtt cggcgagaag
    14281 atcggagaac gataccgtct catccacttg cgacagaacg gaggatggga tcagccgctg
    14341 caattgatcc tctccggttt gatgggccga ctttgcgcag cccgacccca gcatcacgat
    14401 gcagacaaac agggcaacga tgacttgtgt tcggttcata agccggatga atagatggcg
    14461 ttgtcctctt attacaccac cccattcctg aatttaccaa ggggtggctg cgaatgaagc
    14521 tggcgtttcc tccaggtaag tctcgttgcc caggacatct cccaagtggt ccgacttcgc
    14581 ccacagagcg agaaccgtgc aattcccttc gataccatgc atggtaatgg caccgttcca
    14641 catgtcgcac gaatagtggt tgagtccacc taggtagatc ctccgagaat cgatcttttt
    14701 accccttacc aacactctct tcgctgattt tttacatagc acacccgggt tctttgcaac
    14761 cgccggaaag ggtcgcgata tggttgcgcc gccagtacgt gtcgcgccgc gacactcaag
    14821 aacgccaatc caatcgccaa tccctgacga ggttgcccat gaggttgtta ggccggttct
    14881 ttatctttat cctcttgtcg acgtacgccg tggcgttcct tacttcctgc gccggcgagg
    14941 atgcgggctc ttcttctgcg ctcgggacat ttacggctgc aggtggtttc atcgagcacg
    15001 tgcctggcga ccttcgacct cgctggagtg cggcggagtt acgcacattc cttccgacga
    15061 cccgggggaa attcatgttt cccgcaccct acaataccga ggccttccgc atcaccgacg
    15121 catcggattg cggaggcacg gactgcgtga cccctgtcgg ctattcgtac tggcggaaca
    15181 gcaacgcaca cgaaggcagc aacgacatgt ggattttcct aagtctcaat agccagaaag
    15241 gtggttctgg tccgacgctg ttccgattgg acaaggccac cgacagcatt acaaaggtcg
    15301 ggccgctctt tcccacgacc agcaagtttt ccggcagaag tggtgatggc tggtacttca
    15361 gcgccagcag ggccaaccag ctgtacatca atgacggacc aaagctgttg cgctacgatg
    15421 tcaccacgca tacctttgaa acggtcttcg acctcacagg agaattcgga agcgatcgcg
    15481 aggtctggca aatgcactcg agcaacgacg acctcgtcca cagcgccacc ttgcgcgcga
    15541 agggagctgg cacctttttg ggctgtctgg tcttcaccga aaccacagga caattttcct
    15601 atttcgcaaa gaaaggcacg tacgacgagt gccatatcga caaaagcggc cactatctca
    15661 tgattctaga aaatatcgac ggccgcaacg gttcggagaa cgtcatcgtg gatttacaaa
    15721 ccggtatcga gaccgtgatt catgatgaga agggcggcgt ggggcacgct gatatgggct
    15781 acaactatgt ggtcggcgcc gacgggtaca gtcccctgcc caacgccttc atcacgtgga
    15841 gcttcgcacc ttcgttggtc aagggcccaa ctgtgttcca tagttcgagc tcgaaaatcc
    15901 cccgcgtgaa tcacgtctca catcaaaacg ccaagccggg attgccgatg catcaacagt
    15961 tcgcctgcgg cagcagcgcc gatcgagggg cggcacaaaa cgagattttg tgtttcctgc
    16021 tcgacgggac tgagcgggag ctcgtagtgg cgccggtcat gaccgatctc gatgcgtccg
    16081 gaggggggag cgagtactat aaattgccga agggcaatct ggacatcacc ggacagtatt
    16141 ttatctggac gacgaatacg ggagggaacc ggatcgacgc cttcttggtg aaggttcccg
    16201 ctcaactcct gctcagttga actcgttcag ggcgcgcatg ccttcaggca tgccgtccgg
    16261 cgatcacccg atgcgatacg gcgcgggata catgcgcagt caattgaaaa tggacagaga
    16321 gcagagcgat tagaatcccg tgccgatccc gactccgcca aaactgccgc tcctgccgcc
    16381 gcttccgaag ccgaatccca ccccgccgaa gagtccgacg ggccggcgcg atcgttcgcg
    16441 ataaacatcc ggctcccaca catacatgtg cttggcctcg acggtaggat atcgatacgt
    16501 ggattcgtca agccggcgga tctcctcgcc ggtcacggct ccgacaagcg tcaggcgcgt
    16561 gcctggcggt aaggaggccg gatcggtcag ggtccggtcc agtgcgagaa atctgccctg
    16621 ggattcgctt cgtcgttcgg cgggcgggtc gtcggtggtt acagggagtt ggagaatctc
    16681 cagctcggtc ccctcttgca cacgcttggc agccaagact tccccgccca gcaagacggt
    16741 tttcccctgc aggctttgcg gagaggcctg aatgtcgcgg aaggataccg acgaatcgat
    16801 ttgttcctcc agctcggaag ggatcacctg aggacccgag caggctgtcg ccgtgcacag
    16861 caacccgcaa gccacgaggg ggcggaataa catgaggcgc ttcacggttg acatgtccct
    16921 tcctcgctta gaaatggtag gagacgccga acatgacgtt ctgcacctgg taatcgccgc
    16981 tgaatccccc taccggccca aaggcatcat caaacgtcag ggtaccatgg gtgtatttgt
    17041 actcggtgaa gatcgccaca tagggcgtga tgaatgcccg tacgcccgca aggacgttta
    17101 acccgaatcc gacatccgtg tcgcttctgg tcgtcgcact ttccgacagc cgtgcaatca
    17161 gtaacgcgcc accgatcccg acatacggct gaaatgtctg cccgggatat cgcatgagca
    17221 aattgacccc gacgttcgta acgcctaggt gaatgccggg aacgtcatcg agattcttga
    17281 tgttcggttg actgttgaat acatcgagct cgacacccgt cggaccgttg ccagggaaat
    17341 accccagctt tcccccgtag gagacagcat tcttcagatc gaagtcgggt gcccgtagat
    17401 tcaggaggct gccggttcct tcgatgtttt ttagccggtc ggcaaaattc acgccgattt
    17461 gtcctgccac atagtattcc gcttgtgcac ggccaccgag ggacactgaa caggctatcc
    17521 ccgccagtaa gacacaccag gccgctcttg ctctcatgtg ttgagtagct ctgcaattcc
    17581 agggccacac ccgaagtgaa ttgagtcagc agataacagg gcgcgttcct aggaatctcg
    17641 gtcgaacact gccatgccgt ctcgagaggg aggacaatct gtccacttat gaggcgcgtc
    17701 gaagggattg ctccgatcgc acggacgtct ccttggcgag gcgatagcac cagggaagca
    17761 gcacgaacga cacgacggct gcggcgccgt tcactatgcc gatcgtagtc aggctgacac
    17821 caggcccgaa tgaatccgcg agccagccga agagggttat gccgatcatg gaaaacacca
    17881 tcgtgccggt gttaaacagg ccgaacaccc gtgccatcaa atccttcggc gtcctttctt
    17941 gtaacgatgc ggacaccagc ggcgtgatga gacccgatgt cgcgccaatg ccggcaatga
    18001 ggccggcggc gaccagtaca gactcgctta tcagcaatgc cggcacggcg aaggcagcca
    18061 cgccagccgc cacggcaatc atccagatac gtgcgcaaaa cgactgctgc ggcctccaga
    18121 gtaaccacgc ggtcgtgatt aagataccca cgctcagcga cgaccacaac catccgagct
    18181 cgaccgctcc gacgtgcagc agccgatcgc cgatgaccgg taagagatag acaaacgccg
    18241 tcgaccccaa tgtaaagatc gacgcgacaa tcatcaggag gaacagcaga cgctgcttga
    18301 cgaaaacgaa tctgatgccg tcttggaggt cgttcacggc ttgctgccag acattttgac
    18361 tagtccggtg aggacggata tcgggcaggc gcagcggcag cttgcacagc gctgaaatga
    18421 ggaacgcacc ggcattcaca tacaacgcat tctgggcacc gatcgccgca atcaatatgc
    18481 cgctgagggc ggggccgagg agttgtccga tggtcatggc actctgcatc aatgcattga
    18541 tgcgcgtgag ctggtctttt tttacgatga gcggcaacgt ggcgttcaac gccggcccga
    18601 aggccatgga gaacatggca atcacgaata ccagcacata gagtaccggc aaagacaaca
    18661 agcccatggc gtacaa
//
LOCUS       DASSCQ010000219        21431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_36138, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000219 DASSCQ010000000
VERSION     DASSCQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 21431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21431
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36138"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFH05_13675"
     CDS             complement(<1..221)
                     /locus_tag="VFH05_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HET6679320.1"
                     /translation="MTKNDHGTVTETVPELFWRIATDIAQAEQLYPGPTRLVHAARRF
                     YEFMARLEFLPNSPTLMNAGRPLQQLAAC"
     gene            425..838
                     /locus_tag="VFH05_13680"
     CDS             425..838
                     /locus_tag="VFH05_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET6679321.1"
                     /translation="MRSHREELWFETKTRRAYINITRQVESVVRNSGVQEGLVLVNAM
                     HITASVYINDDEQGLLADYDRFLERIAPHDDVYQHNETGEDNGDAHIKRQVMGREVVV
                     AITNGRLDFGPWEQIFYGEFDGRRRKRVLVKVIGD"
     gene            953..1966
                     /locus_tag="VFH05_13685"
     CDS             953..1966
                     /locus_tag="VFH05_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679322.1"
                     /translation="MQSWSRPDPLTRDLIHLINARRHDHGDTDAAIDTLQAFLEQGRE
                     DDLLTVLAALDEDMAEWLFDLLAEASCSIMLDGPADEKLSYAVMAALELPLQANLTHP
                     LKLKIDPVATAEVLHRHLRLSSGTVVRVEPRLLTDATLEALSLQTLNEHLSTVAESQR
                     GRSIAARLYPPVENTAFLFFELIGVPDAGLPDSLEDPHRRALLEGLVEQCPELALAPD
                     TIEAPVYAAYAMFDAQNAVRLHHLADETAAVWRDLDPLVRARTIVHLHTQCGNGVYMP
                     VWTDLFDPELPEEHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVEA
                     PEN"
     gene            2130..2732
                     /locus_tag="VFH05_13690"
     CDS             2130..2732
                     /locus_tag="VFH05_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HET6679323.1"
                     /translation="MSEHTLSNRILAGALTFIALGLIWVAIVSSLPSEQELAAKEAPI
                     VPRAVSKSLPEPTLDLSIPGVPATQPAGVPLTTVAAERPFVSKRLDPRVAQLSRLRCE
                     AEVEQLCPESLDGTARKQCFEQRTPQLSVSCQHQVRQRVVKWKEERSRLMAACQVDIK
                     RFCPMKPGGGELLQCLQQHAQELSDGCYERLPKGALHLKY"
     gene            complement(2739..3440)
                     /locus_tag="VFH05_13695"
     CDS             complement(2739..3440)
                     /locus_tag="VFH05_13695"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6679324.1"
                     /translation="MGTVTNSAKLLVKLQGQGSTLVELERDSFTIGRRPDNDLSIEDH
                     TVSSRHAKIIRVQSVYFLEDLKSTNGTAVNGQAIERAQLHDADVITIGQHRIIFQDSG
                     TIPACASSTSTGFDQTMAIPSRSVQGGPSITARILVTAGKTDRLEYSLTKPSNLIGSQ
                     DGATIRLTGWFAPKTAALISGRAGGFTVSPSQCNKPLLVNGHPVTVQQQLKDGDVIEV
                     AGVSLTFYVVQPNVR"
     gene            complement(3460..4284)
                     /locus_tag="VFH05_13700"
     CDS             complement(3460..4284)
                     /locus_tag="VFH05_13700"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET6679325.1"
                     /translation="MAITRLHFAQTDIGRKRSHNEDCYAADPALGLYVVCDGMGGGNA
                     GEVASHMAIDAILAHVKAEAQRGSVAGRGTVNRNLSPVTNQLADAVRAANETVYRASW
                     EQAKYAGMGTTVVAARLTERHVSVAHVGDSRLYLIRNGAIQTLTSDHSWVAEQVEKGY
                     MTEEEAERSPRRNIVTRALGVESAVDIDLAEIPIANGDLLLLCSDGLTRGVRCRDILN
                     TLEAPGDLCVKVQRLVSQANDAGGDDNITVLLVSVQESAAGRLWHRLAPQWLLNAS"
     gene            complement(4296..5693)
                     /locus_tag="VFH05_13705"
     CDS             complement(4296..5693)
                     /locus_tag="VFH05_13705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6679326.1"
                     /translation="MSKSLHWIIPHASGILAALLLGPLLGSMAWVEFFSIPLTAVTGP
                     NAVRLVTEGIALGLVVAFAVSAYEQLPDNGRGSSFLRSIVLPSAALIVVVFGAKALRS
                     VGLPLIHQMGVSAFVQGYTIGVVGCGLWLTIAWLRHLHLLRNMFSPPAVKRKPARAAE
                     EEESILSGSDDEIAEQATEKAPAIVLNGHRPTSLGRYKILKELGRGAMGLVYLGKDPT
                     IQRFVAIKTMRLDQIDDSEKLQEIRGRFFREAESAGRLSHPNIVTIYDAGEQHDLGYI
                     AMELVEGRSLKEWSRKPHLMPLPELIPTLASVAEALDYAHQQGVVHRDIKPANIMITK
                     ERLVKVMDFGIAKMTSNSKTQTDVVLGTPTYMSPEQIAGKKVDGRSDVFSLGVVIFEL
                     LTGQPPFTADNLSALLFAIAHNPHPELHTLRQDVPPMFEDVLNRALQKELLQRYRRAG
                     ELAQDLRSCLQSLAA"
     gene            complement(5812..6657)
                     /locus_tag="VFH05_13710"
     CDS             complement(5812..6657)
                     /locus_tag="VFH05_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET6679327.1"
                     /translation="MSRIDLHLHTTHSDGSLQPSEVLALAKKANVTALAITDHDITSG
                     ISEAKAAAQHLGIEVIPGVEVSSYDGRSELHILGYYVECDDSLFMSRLASLRASRHRR
                     NPLIIERLREAGLDVTYDEVRALAGTESVGRPHIAQLLMRKKYVSSAKEAFDRYLAEG
                     KPAYVARELPRPEEAIRWIREARGVAVLAHPTWVKDTGDGLRSCITALKEAGLGGVEV
                     HYSTHSKSQTASYLELTQRLGLLVTGGSDFHGITKPDIEVGYGRGDLRVDPALLEPLQ
                     AAARA"
     gene            complement(6654..7604)
                     /gene="folB"
                     /locus_tag="VFH05_13715"
     CDS             complement(6654..7604)
                     /gene="folB"
                     /locus_tag="VFH05_13715"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET6679328.1"
                     /translation="MADKIVIERLEFQGRCGITADERRQPQPMAVDLELDCETRSAGG
                     SDDLADTVDYAAVVERIVNLGEQEICALLESLAEKMLTMLFTEFPVSRARLWLRKLAP
                     PIAHTAGSVGIKVDRGRATHHEQLNHPMPSRFVAQHLHRLPKGRALDVASGIGRHALY
                     LASHGFEVDAIDRDGESLAKLSAEAARRHLTHVETKQVDLERIADERPEFPANTYDAI
                     IVCFYLHRPLFPWLLEALKPQGVLLYETFTIDNYVRHRHPRRWEFCLAQNELLRLTSS
                     LRVLSYDEGEHEGGHGLGSVFTAQLIAQKPRPAVRAHELP"
     gene            7852..8340
                     /gene="moaC"
                     /locus_tag="VFH05_13720"
     CDS             7852..8340
                     /gene="moaC"
                     /locus_tag="VFH05_13720"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET6679329.1"
                     /translation="MAEFTHFNESGRARMVDVSAKAQTERVATAQAKVYLQPETLEKI
                     QRGKIAKGDVLAVAQVAGVMGAKKTPELIPMCHPIFLSAIDISFKEEPQPDRDGRCSI
                     TVTATAKTSGQTGVEMEAMTAVSVAALTIYDMCKAIDRSMSFTDVWLVSKAGGKSGTY
                     TR"
     gene            8386..9084
                     /locus_tag="VFH05_13725"
     CDS             8386..9084
                     /locus_tag="VFH05_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET6679330.1"
                     /translation="MITVKLFGMTKLLAGNESTLTVALAKGHSVKNLIELVNKRYPKI
                     GELIQKKKILVSVNQEIAHEDTKVQEGDEVALLPPFAGGSQSASDLDDSQLVRVQQED
                     FSIDTELKRVRSRSKRIGGIAMFLGTARDRSQGRDVDGITFEHYEGMAQKKLREIRAR
                     ALKDFDILELLIIHRFGAITIGENIVLIIAGAEHRADAFRACKWAIDELKQITPIWKL
                     EHTPTGEVWVEEHP"
     gene            9188..10255
                     /gene="moaA"
                     /locus_tag="VFH05_13730"
     CDS             9188..10255
                     /gene="moaA"
                     /locus_tag="VFH05_13730"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET6679331.1"
                     /translation="MEEHLQQSASDQAVVDAFGRPLRSLRLSVTDRCNLRCAYCMPEE
                     EYVWLPREDVLTFEEMAQLAGYFTDLGVDKVRLTGGEPLLRRDLPRLIRLLLQNHNIK
                     EIALTTNGILLSDHAEALYQAGLNRVTVSLDTLRPERFRQLTRRDEFARVLEGIESVG
                     RAGFTGLKLDTVAIRGFNDDELIGLIEFGKHYQAEVRFIEYMDVGGANEWVPEKVLSR
                     ELILAAVGNYYGSIQPLPERGSAPAQRFQLPDGATFGIIPSTTTPFCATCDRSRVTAD
                     GMWYRCLYATAGTDLRKPLREGVSAAEMLALIRGGWQSRRDRGAEERKALERAGLREG
                     GLIGIDRLREDPHLEMHARGG"
     gene            10452..11159
                     /locus_tag="VFH05_13735"
     CDS             10452..11159
                     /locus_tag="VFH05_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET6679332.1"
                     /translation="MTDPSYLTVLGLGFVLGLRHALDSDHLAAVSTVLAERPSWRASG
                     LVGLSWGLGHTAMLLLVGAIVLFLRVPIPQPVAEVAECVVGLMLVALGGLLGMKLLRE
                     RWHLHQHDHDGTRHVHLHSHAQSQDHGHAHWWRESVRPLCIGMAHGLAGSAALLLFVV
                     ASAGSVLEGLLYIAVFGCGSIAGMMLIGLLLTIPVVWSFRLGRPVFLALQGLASAGSI
                     ALGLAIVYRTLTGAPLS"
     gene            11316..12152
                     /gene="accD"
                     /locus_tag="VFH05_13740"
     CDS             11316..12152
                     /gene="accD"
                     /locus_tag="VFH05_13740"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET6679333.1"
                     /translation="MAWFKKQKGSEPEAPKRSKVAEGMWLKCNHCREIVYRKEVERNS
                     KVCPKCDYHFPISVIERITLLVDFGTFKEWDAELGPLDPLNFHDTKSYKDRVKAQQEK
                     TGKKDAIMIGEGQMNGRRVALCLFDFGFMGGSMGSVVGEKICRAIDRAIQARLPVVLV
                     TASGGARMQEGILSLMQMAKTSAAVAKLGEAKLPLICLLADPTFGGVTASVAMLGDVI
                     IAEPKALIGFAGPRVIEQTIKQQLPDQFQRAEFLLEHGMIDMIVERKQLKETLSTLVA
                     HF"
     gene            12351..13643
                     /locus_tag="VFH05_13745"
     CDS             12351..13643
                     /locus_tag="VFH05_13745"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET6679334.1"
                     /translation="MTYDAAVSYLYNLQKHGIKLGLETMTALIARRGRPDTRYRILHI
                     AGTNGKGSTAAMTAAMLQAAGHRVGLYTSPHLVEFSERIRVDGRMISEADIGRFAEVV
                     QKAAEPDLSPTFFECTTAIALQYFAEAQVDVAVLEVGLGGRFDATNVVTPVACAVTTI
                     ALDHQEYLGTTLSSVAYEKAGIIKPLVPVVVGRMSDEARETIEQVAEERGAPLSRLGR
                     EFAVAGMPERFRFSGRTRRYDNLVCSLSGAHQVDNCACALSLIEAAGERGLDVGETAV
                     REGLRTVQWPGRLEVVDRTPLVVLDGAHNPAAAAAVAEYMQAFRRVNPESRVILVLGM
                     MRDKDHLGFAKSFRGLVDQVVLTQAGLSRSATVDELYGALHELWPSARAEAVLADALT
                     LARRLARTQDLICVTGSLMLIGDVKALRHVGRISPLRG"
     gene            13612..16107
                     /gene="lptD"
                     /locus_tag="VFH05_13750"
     CDS             13612..16107
                     /gene="lptD"
                     /locus_tag="VFH05_13750"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HET6679335.1"
                     /translation="MSEGYPRFGVEMAGTLARVLGLLFGIVIFNLFPVPGQAAGVAGP
                     SASAPASGSPPLEVKADRIDYLQDQDIYEAEGSVVVDQGSVHLTADHLIVQALPGIMI
                     ATGHVRLTDPKADIVAERLELNVNTEAGVITHGEVYMKASNSTLDGRLIQRFSEDHYR
                     FKEGRFTNCDAPEGETPAWRFRFKDLDLNAGDNLAFKGGWFCVADVPVIPIPTMTYPL
                     SPRQSGFLVPTPGYDNRFGFHYQQGYYWAINPSQDLTVSPSYYSNLGYGSDFEYRYVL
                     NRRSRGQWYVSYLQQTTLPNVAGVTDTPGADAKRARALVTGTHIQQITEDLLVRVNAN
                     LVSDPNYLQQLSNSGAQRALPSNESNILANQRLGYGNAYLLGQYLQPLQAGGNDTFQR
                     LPEIGYSQANIPLFDSPLLFGGDANAVNFYREEGFAQNRFDIVPGLSTDVIDVGHVVG
                     FTPQAKFREVYYARGAVDTSSQHRETFWAAMDATSKLSRRFHTDTGEAMLHTIEPSVI
                     YEYVPATDQSNITQIDQVDDLPKKNLMTYMLRSRLLEQDGHSSFNWLDLTLAQSYHVG
                     AVQTRARDFTPGVQPPLGSITQPLQPATVPVEGKRFSDLWLRAVIGNNDPQLTVAQMA
                     GPLFGRGSGTGPLAKPVINRYVTVDAFFDPYRATVSQFNTDFRLQQSNHWYVEVGQRY
                     SREGNRVRRGDIWNPVSFNEVYAPTEEIQFLTAGGAFRTPWGWTVGAKGYYDVKNGRS
                     PEYDVVALYQNPCRCWSLGLFYLQFPDRAQYNFMLSLTGIGWTENYGTNVVRTILSPL
                     VWGEKGLPWASPTGPYGRPETIQPQPRSRTAPR"
     gene            16104..16646
                     /locus_tag="VFH05_13755"
     CDS             16104..16646
                     /locus_tag="VFH05_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679336.1"
                     /translation="MNRSVRMSAYGSVVLWTMVLLVWPGCRAVSSTPDPVHSPHLQRA
                     RVFLAAGDFRRAVEACRQEVWEHPSARSYLYLTYVYQALDAYVESLAKSDYWVGMEHL
                     YLNLTSGSPEELLDPPNVLARMAKEMIQAATRQQADVTSELATRLDPAVVAVVWPQQT
                     AWRKARPDGWWFSVPAEWPW"
     gene            16745..17092
                     /gene="trxA"
                     /locus_tag="VFH05_13760"
     CDS             16745..17092
                     /gene="trxA"
                     /locus_tag="VFH05_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6679337.1"
                     /translation="MAGNALKVEDATWDSEVMKAAELVMVDFWAVWCGPCQMVAPIVD
                     ELATEYAGKVKVRKLNTDENPEVAGRYQVMSIPTILFFKNGQVVEKLVGARPKRQFKE
                     MIDQLLAQHAGSA"
     gene            17339..19024
                     /gene="recN"
                     /locus_tag="VFH05_13765"
     CDS             17339..19024
                     /gene="recN"
                     /locus_tag="VFH05_13765"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET6679338.1"
                     /translation="MLAELRIVNFALIEQLHIQFQSGFTVLTGETGAGKSLLIDAIGL
                     LVGGRASAEQIRSGEDEAHLEAAFHLPLSHPLLAHMRAQDLIGARETDLILKRILSRS
                     GRHRVYVNGSLCPLRVLEDLGGTLVDIHGQHEQQSLLASTQQLHAVDAFGSTATLRTQ
                     YETAYREWRDLTADIDALRCAGVERARMEELLRFQVQEIEQAALSVDEEDRLNMERQR
                     LSHAHRLRELAESSYAVLQEDERATLPQLATVARFVSELVQTDPGMEECEVQVRQAII
                     HLKDVTARLRGYADDLEADPARQATVEDRLDLIHRLKTKYGGSTEAVLETGKRARQEL
                     EMLEQHEAQMSGIVARLKEAESRVIDKAKQLTKKRREAAKRLTALVAKELAAVRMAHT
                     LFDIVVTADEQLVRFGPTGRDHLEFLLSSNVGEPPRPLNRVASGGELSRIMLALKTVL
                     AETDQVPVLVFDEIDTGVGGAVAAAMGTRLRKLGAYHQVFCITHLPQVASQAEHHVIV
                     EKGQDGRRTTTSVRLLSGVDREEEIARMLGGETVTKKIRETAAELIAGAKSKR"
     gene            complement(19108..20259)
                     /locus_tag="VFH05_13770"
     CDS             complement(19108..20259)
                     /locus_tag="VFH05_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET6679339.1"
                     /translation="MTIPALRNVPAQKPTVLVIDDEAGPRDALKVILRPFFNIQSAES
                     AQAAINVLNHQQIDLITLDQKLPDRQGMDLLQEIKQDHANIEVIIITGYGSLKSAMEG
                     IRHGAAGYLLKPFNVTELITLVNQTLEKKQRLDYLRRFLATSTGLWGTDRECAQAWHE
                     MREGYEAIGKCSGRGLSAHDDASNLLPMLSDLLEAKDRQLLNHCSRVSFYATLLANRT
                     NLTVEEQKSLALGAFLHDIGKVGKEAYRFADDEIVATNTTHELRSHPEIGAKLVAPLG
                     LPAATGQIIAYHHERWDGSGYPHGLKGEGIPILARIVCLAQTFDHLTAELPDRIALPI
                     DDASSFISARAGSYFDPQLTGLFTQVVQECKASLPAMAIATSPSTRSDV"
     gene            complement(20462..>21431)
                     /locus_tag="VFH05_13775"
     CDS             complement(20462..>21431)
                     /locus_tag="VFH05_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6679340.1"
                     /translation="QEIYGKIEQVADSRTTVLITGESGTGKELVAKALHYNSGRRDRP
                     FVALNCAALPETLIESELFGHEKGSFTDATARRVGQFELANSGTLFLDEIGDLSAMTQ
                     AKLLRVLQEREFTRIGGVQAIKVDVRIVAATNKNLEELVRKGQFREDLYYRINVIALY
                     LPPLRERGEDVPLLAKHFLSKRIEEDHRPSQDFSKEAIDLLARYPWPGNVREMENIIE
                     QAFIWSKGSDVVTPEHLPTILRNDTRSSSLRDDTLAGRLSLEKAVMEFEREIILDALK
                     KNNYVQTHAASQLGISRRMLKYRMDTLGIGRPDGESPPEPEPVAQE"
BASE COUNT         4363 a         5995 c         6496 g         4577 t
ORIGIN      
        1 aaacaggcgg ccaattgctg cagtggtcgt ccagcattca tgagcgtggg cgagttggga
       61 agaaattcca gccgagccat gaattcgtaa aaacggcgcg ccgcatgcac gagtctggta
      121 ggaccgggat agagctgctc agcttgcgcg atgtcggtgg cgatgcgcca gaacagctca
      181 ggaacggtct cagtaaccgt gccgtgatca ttcttcgtca aataacgtcc acgaagcacc
      241 agcttcgctt ctgctgacag ccgcggaaca ggcgatgtcg gcggcgcgga tgatggtaga
      301 gcggggaagg aacgcatgcg acattatagt cgccgcgggc cgggtttgac gatatgcgcc
      361 gcccgatgcc ggggattgca tcttcaggct gttgggacgc agaatgcacc tcggtccatc
      421 cgctatgcga tcgcaccgtg aagagctctg gttcgaaacc aagacccgcc gcgcgtacat
      481 caatatcacg cggcaagtcg aatctgtcgt caggaacagc ggtgtgcagg aaggtctcgt
      541 gctggtcaac gccatgcaca ttaccgcgag cgtctacatc aacgatgacg agcaaggcct
      601 gctggcagat tacgaccgct ttttggagcg tatcgcgccg catgatgacg tgtatcagca
      661 caatgagact ggcgaggaca acggggatgc tcatatcaaa cggcaggtga tggggcggga
      721 agtcgtcgtc gccatcacga acggccgcct ggattttggc ccatgggaac aaatttttta
      781 cggcgaattc gacggccggc gccgcaaacg agtgttggtg aaagtcatcg gagactgaca
      841 tgccggcgtc caatctgcgg ctcggtccga atgcagttga gcgcttgctc cctgatggaa
      901 agtttggcaa catggaagtc aaccccctgt tcgtgagtgc tggagcattg ccatgcaaag
      961 ttggtctcgt ccggatcccc tgaccagaga tctgatccat ctcatcaacg cgcgccggca
     1021 cgatcacggc gacaccgatg cggccatcga tacgttgcag gcctttctcg agcaaggccg
     1081 tgaggatgac ttgctgacgg tgttggctgc gctggatgaa gatatggccg aatggctctt
     1141 cgatcttctc gctgaagcgt cctgttccat catgctggat ggaccagcgg acgaaaaact
     1201 gtcctatgcg gtcatggccg cgctggagct tccgctccag gccaatctta cccatccatt
     1261 gaagctcaag atcgatccgg tcgcgacggc tgaagtgctg catcgacatt tgcggctgtc
     1321 ctcaggaacg gtcgttcgcg tcgaaccgcg cttattgacc gatgccaccc ttgaagcctt
     1381 gagtctgcaa accctgaatg agcatctcag cacggtggcg gaatcgcaac gggggcgttc
     1441 aattgcggca cggctctatc cacccgtcga aaacaccgcc tttctcttct ttgagttgat
     1501 cggtgtgccg gatgccggcc tccctgactc cctcgaagac ccacatcgtc gggcgctcct
     1561 ggagggattg gtggagcaat gtcctgaact ggcgctcgct ccggatacta ttgaggcgcc
     1621 ggtgtatgca gcctatgcca tgttcgatgc gcaaaacgcc gtccggcttc atcacctcgc
     1681 cgacgaaacc gcggccgtct ggcgcgacct tgatccgctt gtccgtgcga gaacgatcgt
     1741 tcacttacat acacagtgcg ggaacggcgt ctatatgccg gtgtggacgg atttgttcga
     1801 cccggagctg cctgaagagc atggccctgt ctggacctcg ccggatgtct gggtattcac
     1861 cgccgacctg ccaatcgagt ggccaggtga aatgctcacc aatcgtctac tggcagaggg
     1921 ttgcaccagg ttcgaaaacc atatcgtcga ggctcccgag aactaggagg ccggctccgg
     1981 cgaccggtgc ggtgacagcc agcgagcgga aaggtggctg ttcccttttc taatcattcg
     2041 tcctactgcc ctgccaaatc cccacatttc gtcgattttt tgtgtgcctt gccctatgct
     2101 gaaatctgtt ggggaagaga cacatcatca tgagcgagca cacgctttcc aaccggattc
     2161 tggccggcgc gctcaccttt atcgcgctgg ggctgatctg ggttgccatc gtcagcagcc
     2221 tgccttcaga gcaggagctc gctgcgaagg aagcgccgat cgtaccgcgg gcagtgtcga
     2281 aatcccttcc tgaacccaca ctcgatctgt cgattcccgg tgtgccggca actcagcctg
     2341 ccggagtgcc gctgacgact gtcgccgcag agaggccgtt cgtctccaag cggcttgatc
     2401 ctcgtgttgc ccaactatct cgactgcggt gtgaagccga agttgaacag ctctgccccg
     2461 aatctctgga tggaaccgct cgcaaacagt gctttgagca gcgcacgccg caactgtccg
     2521 tgtcttgtca gcatcaggtg cgccaacgcg tggtcaaatg gaaggaagag cggagccgct
     2581 tgatggccgc ctgtcaggtc gatatcaagc gattttgtcc catgaaacca ggaggcgggg
     2641 aattgctgca gtgtctccag caacatgccc aggagctgtc ggacgggtgt tatgagaggc
     2701 ttccgaaagg ggcgttgcac ctcaagtact agaatccgct atctgacatt cggctgcacg
     2761 acatagaatg tcaacgagac cccggccacc tcgatcacat cgccatcctt caactgctgt
     2821 tgcacggtca cgggatgtcc attcacgagc agcggtttgt tgcactgcga cgggctgacg
     2881 gtaaaaccgc ctgctcgacc ggaaatcaat gcggccgttt tgggagcgaa ccagccggtc
     2941 aagcggatgg ttgcgccgtc ctgcgacccg atcagattag agggcttggt gagcgaatac
     3001 tctaaacggt ctgtctttcc cgcggtgacc aggattcttg ccgtgatcga cggcccacct
     3061 tgcacagatc tgctaggaat ggccatcgtt tgatcgaacc ccgtagaggt cgatgaggca
     3121 cacgcaggga tcgtaccact gtcctgaaaa atgatgcgat gctgaccgat cgtgatgacg
     3181 tcggcgtcat gcagttgcgc acgctcaatg gcttggccgt tgaccgccgt gccgttcgtg
     3241 ctcttaagat cttccaggaa gtagacggat tgcactcgga taatcttcgc gtggcggctt
     3301 gacacagtgt gatcttcgat cgataaatcg ttatccggcc ttcgaccgat cgtaaaggaa
     3361 tcccgttcaa gctcgaccaa agtggagcct tggccctgga gtttgaccag aagtttcgcc
     3421 gagttggtga cggtgcccat cgtgcgctcc ggatgaaggt caagacgcgt tcaacaacca
     3481 ctgtggcgcg aggcggtgcc acagtcgacc tgccgcgctc tcctggacgc ttaccaacaa
     3541 cacggtgatg ttgtcgtctc caccggcgtc gttcgcctgc gagaccaatc gctgcacttt
     3601 gacgcacaga tccccgggcg cctctaaagt gttgaggatg tcgcggcagc ggacgccccg
     3661 ggtgagaccg tctgagcaca gcagcaggag gtcaccgttc gcgatgggga tctcggcgag
     3721 atcgatgtca acggcgctct cgacgcccaa cgctcgagtg acaatgttcc ggcgcggtga
     3781 acgctccgct tcctcttcgg tcatgtagcc tttttccact tgttcagcga cccacgaatg
     3841 gtcgctggtc aaggtctgta tcgcaccgtt gcggatcaag tacagccgac tgtctccgac
     3901 gtgggcgacg gacacatgcc gctcggtaag gcgggcggcg acgacggtgg ttcccatccc
     3961 ggcgtacttc gcttgttccc aagaagctcg atacaccgtt tcgttggcgg cgcggacggc
     4021 atcggccagc tggttggtca cgggagataa attgcggttg accgtgcctc gaccggcgac
     4081 agaccctctt tgcgcctcgg ctttcacgtg ggcgaggatg gcgtcgatcg ccatgtggct
     4141 tgccacttcg cccgcattgc cgccccccat cccgtcacag acgacataga gaccgagtgc
     4201 cggatcagcc gcatagcagt cttcgttatg agaccgcttg cgtccgatgt cggtttgggc
     4261 gaagtgaagc cgggtgatcg ccacaagcgc ttctcctagg cggctagact ttgcagacag
     4321 gagcggagat cttgcgcgag ctctccggcc cggcggtaac gctggagaag ctctttttgc
     4381 aaggctcggt tcaacacgtc ctcaaacatt ggaggcacgt cctggcgcag cgtgtggagc
     4441 tcgggatgcg gattgtgcgc gatggcaaac agcagggccg acaggttgtc cgcggtgaac
     4501 ggcggttgtc cggtcaacag ctcgaaaatc acgaccccga gtgaaaagac atcagaccgt
     4561 ccatcgactt ttttcccggc gatctgctca ggcgacatat aagtgggcgt gcccaggacg
     4621 acatcggtct gggtcttgct gttcgatgtc atttttgcga tgccgaagtc catgaccttc
     4681 acgagccgct ccttggtgat catgatgttc gcgggtttga tgtcgcggtg tacgacgccc
     4741 tgttggtgcg cataatcgag ggcctcagcg acggaggcga gcgtcgggat gagttcgggc
     4801 agcggcatga gatgcggttt gcgggaccac tctttcagcg accgaccttc gaccagctcc
     4861 atcgcgatat agcccaggtc atgctgctcg ccggcatcgt aaatggtgac gatgttggga
     4921 tgagacaggc gtcccgccga ttcggcttcc cggaagaaac gtccccggat ctcctgcaat
     4981 ttctccgagt cgtctatctg atccagccgc atggtcttga tggcgacgaa acgttgaatc
     5041 gtggggtctt tcccgagata cacgaggccc atcgcaccac gtcccaattc cttgagaatt
     5101 ttgtagcgcc ccaggcttgt cggacggtgg ccattgagga cgatggccgg ggctttctcc
     5161 gttgcctgct cggcgatctc atcgtcgctg cctgacaaga tcgactcctc ctcttctgcc
     5221 gctcgcgcgg gttttcgctt gactgctggc ggcgaaaaca tgttccgtaa gagatgcaaa
     5281 tgtcgaagcc acgcaatcgt cagccacagc ccgcacccga cgactccaat cgtatagcct
     5341 tgcacaaacg ccgatacccc catctgatgg atcaacggca ggccgaccga gcgcagcgct
     5401 ttcgcgccaa ataccacgac gatcagcgcg gctgacggaa ggacgatgga gcgaagaaag
     5461 ctcgatccgc ggccgttgtc tggaagctgc tcatacgcag agaccgcaaa cgcaaccacc
     5521 agcccgagtg cgatgccttc ggtgaccaac cgcaccgcgt tgggtccggt caccgcggtc
     5581 aagggaatgc tgaagaattc gacccaggcc atactgccga ggagggggcc gagcaggaga
     5641 gccgcgagaa tcccgctcgc gtgagggata atccaatgca agctttttga catggttttg
     5701 atcgggacga ttgaatggtc ttcagtctaa cggacggcgg tgaagagtca atgaatacca
     5761 ggcaaattgc ggggatggac gcttgtgaga gacggttctg agacggctac cttaggctct
     5821 tgcggcggct tggagcggct ccaataacgc gggatccacg cggagatccc cacgtccgta
     5881 tccaacctca atgtccggct tcgtgatccc atgaaagtcg ctgccgccgg tcacgagcaa
     5941 gccgagacgt tgtgtcaatt ccaggtaact ggccgtttga gatttgctgt gcgtgctgta
     6001 gtgcacctcg actcctccta ggcctgcctc cttgagtgcg gtgatgcagg agcggagtcc
     6061 gtcgcccgtg tctttcaccc atgtgggatg cgccaacacg gcgacgcccc tggcctcgcg
     6121 tatccatcga atcgcttctt ccggcctagg cagttcccgg gcgacgtagg cgggtttccc
     6181 ttcggcgaga tagcggtcga aggcctcctt ggctgacgac acatatttct ttcgcatgag
     6241 cagctgagca atatggggtc gtccgaccga ttcggtcccg gccagcgccc gcacctcgtc
     6301 atatgtcaca tcgagaccgg cttcacgaag acgttcgatg ataagcggat tgcgtcgatg
     6361 tcggctggct cgaagggaag ccagccgact catgaatagg ctgtcgtcac actcaacata
     6421 gtagcccaga atgtgcagtt cgctgcggcc gtcataggag ctgacttcca cccccgggat
     6481 cacttcgatg ccgaggtgtt gagcagcggc cttggcttcg gaaataccgc tcgtgatgtc
     6541 gtggtcggtg atcgccagcg cggtcacgtt ggctttcttg gccaatgcca acacctcaga
     6601 cggctgcaag cttccgtccg aatgggtggt atgaaggtgg agatcgattc gactcacggc
     6661 aactcgtgcg cccgtacggc tggtctcggt ttttgggcaa tcagttgagc ggtgaagacg
     6721 gagcccaatc cgtggcctcc ctcatgttcg ccttcgtcgt aggagagtac gcggagggag
     6781 gaggtcagac gaagcagttc gttctgagcc aaacagaact cccagcgccg gggatggcga
     6841 tgacgcacgt agttatcgat ggtgaaggtt tcatagagga gcacgccctg cggcttcaag
     6901 gcctccagga gccagggaaa taacgggcgg tgcagataga aacacacgat gatcgcatcg
     6961 taggtattgg cgggaaattc cggtcgctcg tcggcgattc gttcgaggtc cacctgcttg
     7021 gtctcgacgt gggtcagatg ccgcctggcg gcctcggccg acagtttggc gagactctcg
     7081 ccatcgcgat caatggcgtc gacttcaaaa ccgtggctgg cgagatacag agcatggcgt
     7141 ccgattccgc tcgcgacgtc caatgcccgg cctttcggta agcggtgcag gtgctgggca
     7201 acgaagcggg atggcatggg atggttgagt tgctcatggt gggtggcacg gccccggtcc
     7261 actttgatgc ccaccgatcc agcggtatgg gcgattggag gggctaactt acggagccag
     7321 agccgcgcgc ggctgaccgg aaactccgta aacaacatcg tcagcatctt ttcagcaagg
     7381 ctctcgagga gggcgcagat ctcttgttcc cccaagttga cgatccgctc gaccaccgcg
     7441 gcataatcca ccgtgtcggc caaatcgtcc gagccccccg cggatcgggt ttcacagtct
     7501 agctcaagat cgacggccat cggctggggt tgccgccgct cgtcggcagt aatgccgcat
     7561 cgcccttgga attccaaccg ttcgataacg attttgtcgg ccattgttgg cattttccgg
     7621 gatgccaaga gaagctgtca acctgtcgta ggaaggggga ggctccaacg caggcggccg
     7681 tcaaatgaat ggaaagaatc ggaccccgat gtggtcgccc tccggggaaa agccgacctc
     7741 cgatgaagtg cttctgttag agttggatcg aattcgcgcc ttgacgtgcc gttcgtgccc
     7801 ttcatataat gaccggtcgc cgcaatctaa ccggctgatt cgccgaggaa aatggccgag
     7861 ttcacacact ttaacgagtc gggtcgggcg cggatggttg atgtgagcgc caaggcccag
     7921 accgaacgag tggcgaccgc ccaggcgaag gtctacctcc aacccgaaac tcttgaaaag
     7981 atacaacgcg gaaagattgc gaaaggggat gtactcgccg tggcccaagt ggcaggcgtc
     8041 atgggggcca aaaagacccc cgagttgatt cccatgtgcc atccgatctt tctctcggcg
     8101 atagatatct cgttcaagga agaaccgcaa cccgatcgtg atggacgctg ttctatcacc
     8161 gtgacggcca cagccaagac gagcgggcag acaggggtcg agatggaagc gatgactgcc
     8221 gtctctgtcg ctgctctgac catctacgat atgtgcaagg cgatcgatcg gtcaatgagc
     8281 ttcaccgatg tgtggctcgt gtcaaaggcc ggcggcaagt ccgggacgta caccagatag
     8341 tatgcggacg acgagggccg gcgaacctcg gaggagtgag tgtcgatgat caccgtcaag
     8401 ttattcggca tgacgaagct gcttgcgggg aacgaatcta ccctgaccgt tgccctggcc
     8461 aagggacact ccgtgaaaaa tctcattgaa ttggtgaaca agcggtatcc caagatcggt
     8521 gaattgatcc agaagaaaaa aattctggtc tcagtcaatc aggaaatcgc ccacgaggat
     8581 accaaagtcc aagaagggga tgaggtcgcg ctgctgcctc cattcgccgg cggatcccaa
     8641 tcggcctcag acctcgatga cagtcaattg gtacgcgtgc agcaggagga tttttcgatc
     8701 gatacggaat tgaaacgtgt cagaagccga tccaagcgca tcggcggcat tgccatgttc
     8761 ctcggcactg cccgcgaccg ttcgcaggga cgtgacgtcg atggcattac cttcgagcat
     8821 tacgagggga tggctcaaaa gaaactgcgg gaaatacggg cgagggcgtt gaaggatttc
     8881 gacattctcg aattgctgat cattcatcgc ttcggggcaa ttacgatcgg tgaaaacatc
     8941 gtcttgatca tcgcgggcgc ggaacaccgt gctgacgcgt ttcgggcgtg taaatgggcc
     9001 atcgacgagt tgaagcaaat cactccgatc tggaagctgg agcatacgcc gacaggcgaa
     9061 gtctgggtcg aagaacaccc atgaggatgg cgaaacttcg ggcttgcttg ggcgagtgcg
     9121 cgtctcgacg cgccaggggt gggcgggtga gacatgaccg cagtgtgacc atcctctata
     9181 ccgcatgatg gaagaacacc ttcaacagag tgcttcagat caggccgtcg ttgacgcctt
     9241 tggtcggccg ctgagaagtc tgcgcctgtc cgtgaccgac cggtgcaact tgcgatgcgc
     9301 gtattgtatg cctgaggaag aatatgtgtg gttgccccgg gaggacgtgc tcaccttcga
     9361 ggaaatggcc cagctggccg gctactttac ggatctcggc gtcgacaaag tcagattgac
     9421 tggtggagag ccgttgctgc gccgcgatct cccgagactg attcggttgc ttcttcagaa
     9481 tcacaacatc aaagagattg cactgacaac caatgggatt ctcctgtctg accatgccga
     9541 ggcgctgtat caggccggtt taaaccgcgt gaccgtcagt ctcgatacac tgagacccga
     9601 gcgcttcagg cagctcacac gaagggacga attcgcccgg gtcctcgaag gcattgagtc
     9661 agttggtcgg gcgggcttta cgggactcaa gctcgatacc gtggcgattc gaggcttcaa
     9721 cgacgacgag ttgatcgggc tgattgaatt cggcaagcat tatcaagccg aagtgcggtt
     9781 catcgagtac atggatgtcg gcggcgcgaa cgaatgggtt ccggaaaagg tcctgtctcg
     9841 agagttgatc cttgcggcag tgggcaacta ttacgggagc attcaaccct tgccggagcg
     9901 aggatcggct cccgctcaac gcttccagct tccggatggc gcgacattcg gcatcatccc
     9961 gtccaccacg accccattct gtgcaacctg cgaccgcagc cgggtgacgg ccgacgggat
    10021 gtggtatcgc tgtctctacg cgacagcggg caccgacttg cggaaaccgc tgcgcgaggg
    10081 tgtgtcggct gcggagatgc tggccttgat tcgaggcgga tggcaatcgc ggcgagaccg
    10141 cggggcggaa gaacggaagg cactggagcg cgcggggttg cgggaaggcg ggctgattgg
    10201 tatcgatcgt ttgcgggaag atccgcatct ggaaatgcac gcgcgcggtg gttagccgca
    10261 gcgaaatgcc tcccggcgcc gcaccagcat cgctgggtgc ttggcgattt cagatcggtg
    10321 tcctagctgg cccttcagtc gcggccctgc cggaccgcct gatgcccatc ctgcgagatg
    10381 acacttcatg ccgcttattt tccgttccct ctcggtttca cgcgcaccag ccgatggact
    10441 aatcctgccc catgactgac ccttcctact tgacggtgct cggactgggc tttgtgctcg
    10501 gcctccgtca tgcattggat tcagaccatc tggcggctgt ctcaacggtg ctcgcggagc
    10561 ggccgtcatg gagggcctcg ggtctcgtcg ggctgagttg gggactcggt cacacggcga
    10621 tgttgctgct tgtcggagcc atcgtcctgt tcttgcgcgt cccgattccc caaccggtcg
    10681 cagaggtggc ggaatgtgtg gttggactca tgctcgtcgc gcttggcggt ctgctcggca
    10741 tgaagttgct gagagagcgc tggcatctgc atcaacacga ccatgacggt acgaggcatg
    10801 tgcatcttca cagccatgcg cagtcgcaag atcacggtca cgcgcattgg tggcgcgagt
    10861 ccgtgcggcc gctctgcatc ggaatggccc atggtctcgc cggctctgcc gcactgttgc
    10921 tgtttgtcgt cgcatcggca ggcagtgtcc tggaaggatt gctctacatt gccgtcttcg
    10981 gctgcggttc tattgcgggc atgatgttga tcggattgct cctgactatt cccgtcgtgt
    11041 ggtcctttcg gctggggcgc ccggtatttc ttgcactcca gggactggcc agtgccggca
    11101 gtatcgcgtt gggtctcgca atcgtctatc gcacgcttac cggcgcgccg ctgtcctaga
    11161 cgagatccct cacttaccgc gcgagggtga ttctacgtgc gccgcgcgct tcgctatggt
    11221 attctccaca cccaactgac gggttctctt gcgacaggca agttccttga ctcctcagac
    11281 ggcccagatt gattgattca tagaaggagc ctgccatggc gtggttcaag aaacaaaagg
    11341 gctcggaacc ggaagcccct aaacggtcaa aagtggccga aggaatgtgg cttaagtgca
    11401 atcattgccg tgaaatcgtc tatcggaaag aagtcgagcg caatagcaaa gtttgcccga
    11461 aatgcgacta tcattttccc atttcggtta tcgagcgtat cacccttctg gtcgacttcg
    11521 ggaccttcaa agaatgggat gccgaactcg gcccgttgga tccattgaac tttcacgata
    11581 ccaaatcgta caaagaccgg gtcaaggctc agcaggagaa gacggggaaa aaagacgcca
    11641 tcatgatcgg cgaaggccag atgaacggcc ggcgagtggc gctctgtctc ttcgacttcg
    11701 gattcatggg cggcagcatg ggttctgtcg tgggtgaaaa gatttgccgt gcgatcgatc
    11761 gcgcgattca agctcgcctt cccgttgtgt tggtgaccgc ttccggcggt gcccgcatgc
    11821 aggaaggcat tctgtccctc atgcagatgg ccaagacgtc agcggcggta gccaaattgg
    11881 gagaggccaa gcttcccttg atctgtctcc tggcggatcc aacgttcggc ggggtgacgg
    11941 cgagtgtcgc catgcttggc gacgttatca ttgccgaacc gaaggctctg atcgggtttg
    12001 ccgggcctcg ggtgatcgaa cagaccatca agcagcaact gccggaccaa tttcagcgag
    12061 ccgagtttct gttggaacac gggatgatcg acatgattgt cgagcgcaag cagctcaagg
    12121 agacgttgag cactctcgtc gcccatttct agcagagtgt tgacacagcc gtattcccac
    12181 ccgcccagcc ctggcgtacg gtcttaccca cccgtaccca ccgattgcta cgcaatcgat
    12241 accccgcgac gctcctttct ccaggcgtcg ttcctgcgtc gcctcagaat ccaccgtccg
    12301 gctgggaaat ggactgctcc cacgggtgat gggaagacgg atgaacgccc atgacgtacg
    12361 acgctgccgt ctcgtatctc tacaaccttc agaaacacgg catcaaactc gggctcgaga
    12421 cgatgacggc gttgatcgcc cggcgtgggc ggccggatac ccgctatcgc atcttgcaca
    12481 tcgccggtac gaatgggaaa gggtcgacgg ctgcgatgac ggctgccatg ttgcaggcgg
    12541 ccggacatcg ggtgggattg tatacgtcgc cccacctcgt tgaattcagt gaacgcatcc
    12601 gagtggacgg ccggatgatc tctgaagcgg acatcggccg gttcgcggag gtcgtgcaga
    12661 aggccgccga gccggacctg tcgccaacct tctttgagtg tacgaccgcc atcgccctcc
    12721 agtattttgc cgaggctcag gttgatgtgg cggtcttgga agtcggcttg ggaggccgat
    12781 ttgatgccac caacgtcgtc acgccggtcg cctgtgccgt cacgacgatc gccttggatc
    12841 atcaagagta tctgggcacg acgctgtctt cggtcgcgta cgagaaagcc ggcattatca
    12901 agccgctcgt cccggtcgtg gtcgggcgga tgtccgacga ggcgcgagag acgatcgagc
    12961 aggtcgcgga ggaacgtggc gcgccattga gccgattggg tcgggagttc gcggtggcag
    13021 gaatgccgga gcggttccga ttctcggggc gtacgcgtcg gtatgacaac ctcgtttgct
    13081 cgttatcagg agcgcatcag gtggataact gtgcctgtgc cctgtctttg atcgaagcag
    13141 ccggtgaacg aggattggac gtgggagaaa ctgccgtccg agaaggttta cggacggtgc
    13201 aatggccggg acgtttggaa gtcgtggatc ggacgccgct ggtggtactc gacggcgccc
    13261 acaatccggc ggccgccgct gccgtcgctg agtatatgca ggcctttcgt cgggtgaatc
    13321 ccgaaagccg tgtgatcctc gtgctgggca tgatgcggga caaggaccat ctcggattcg
    13381 cgaagtcttt ccggggactg gtcgaccagg tcgtcctgac acaagccggt ctctcacgct
    13441 cggccacggt cgatgagctc tacggtgcgt tacacgagct ttggccttcg gctcgcgccg
    13501 aagcggtgtt ggccgatgca ctgacccttg ccagacgcct cgcacggact caggatctca
    13561 tttgtgtgac cggctcgttg atgctcatcg gggacgtgaa agcgttgagg catgtcggaa
    13621 ggatatcccc gcttcggggt tgaaatggcg ggaacgttgg cgcgagttct cggcctcctg
    13681 ttcggcatcg tgatcttcaa tctctttccc gtgccgggcc aggcagcagg tgtcgctggt
    13741 ccttctgctt ccgctccagc ttcaggatca cctcctctcg aagtgaaggc cgatcgaatc
    13801 gactatctcc aagatcagga catctatgag gcggaagggt cggtggtcgt cgatcaaggg
    13861 tccgtccatc tcacggcgga ccatctgatt gtccaggcac tgccgggcat catgatcgcg
    13921 acggggcatg tcagattgac ggatccgaag gccgatattg tcgctgagcg gttggagctg
    13981 aacgtgaata ccgaagcggg tgtgattacg cacggggaag tctatatgaa ggcctcgaat
    14041 tcgacgctgg acggacgact gattcaacga ttttcggaag accattatcg cttcaaggaa
    14101 ggccggttta ccaactgcga cgcgccggag ggagaaacgc cggcctggcg atttcggttc
    14161 aaggatctgg atctcaacgc cggagataac ctcgcgttca aaggcgggtg gttttgcgtg
    14221 gcggacgttc cggtgattcc catccccaca atgacgtatc cgctttcgcc gagacaaagc
    14281 ggatttctag tgccgactcc gggctatgac aatcggttcg gcttccacta tcaacagggc
    14341 tactattggg ccattaatcc gagtcaggat ctcacggtct caccgtccta ttatagtaat
    14401 ttaggatatg gcagcgattt tgagtatcgc tacgtcctca acaggagatc cagaggccaa
    14461 tggtatgtca gctatctgca gcagacgacg ctgcccaatg tcgccggtgt gaccgatact
    14521 cccggagcgg atgcgaaacg ggcacgggcg ctcgtcaccg gaacgcatat ccagcaaatc
    14581 accgaggatc tcttggtgag ggtcaatgcc aatctcgtgt ctgatccaaa ctatcttcag
    14641 caattgagca attctggtgc ccagcgcgcg ctgccgagca atgaatccaa cattctggcg
    14701 aatcagcggt tggggtacgg caacgcgtat ctcctgggcc agtatctcca accgctacag
    14761 gctggcggca atgatacatt tcaacgactt ccggaaatcg gatacagcca ggcgaatatc
    14821 ccgctgttcg attctcctct gttgttcggg ggggatgcga atgcggttaa tttttatcgg
    14881 gaggaaggat tcgcgcaaaa ccgcttcgat atcgtgccgg ggttgtccac cgatgtcatc
    14941 gatgtaggac atgtcgtcgg ttttacgccg caggccaagt tccgcgaagt ctattatgcg
    15001 cggggggctg tcgataccag cagccaacat cgagaaacgt tttgggcggc catggacgca
    15061 acgtctaaat tgagccgtcg ctttcatacg gacaccggcg aggcgatgct gcacaccatt
    15121 gagccgagcg tcatctacga gtacgtcccc gcgacggatc agtcgaacat cactcaaatc
    15181 gatcaggtcg acgatctccc aaagaagaat ctcatgacct atatgctgcg cagccggctg
    15241 ttggaacaag acggccatag cagctttaac tggctcgatc tgaccttagc gcagagctat
    15301 cacgtgggcg ccgtgcagac acgggcaagg gatttcacgc ccggcgttca gcctccactc
    15361 ggcagcatca ctcagcctct ccagccggca acggttccgg tcgagggcaa gagattttcc
    15421 gatctctggt tacgggccgt catcgggaat aacgatccac agctcacggt cgcacaaatg
    15481 gcgggccccc tgttcggtcg gggatccggc accggaccgc tggccaagcc ggtcatcaat
    15541 cggtacgtta ctgtggatgc attcttcgat ccctatcgtg ccaccgttag ccagttcaac
    15601 accgactttc gattacagca atccaatcac tggtatgtcg aagtgggcca gcgctattct
    15661 cgggaaggca accgagtacg ccgcggtgac atctggaatc cggtgtcgtt caacgaggtg
    15721 tatgccccga ctgaagaaat tcaattcttg accgcgggcg gtgcttttcg aacgccgtgg
    15781 ggatggaccg tcggggccaa gggttactat gatgtcaaga acggcaggag cccggagtat
    15841 gatgtggtcg ccctgtacca gaatccctgt agatgttggt cgctcggctt gttctatctc
    15901 caattccccg accgcgccca gtataatttc atgctcagcc tgaccggaat cggttggaca
    15961 gaaaattacg gaacgaatgt cgttagaacc atcctgagcc ccctggtttg gggagagaaa
    16021 ggattaccgt gggcttcacc gactggaccc tacggacggc cggagactat tcaacctcaa
    16081 ccccgctcaa ggactgcgcc tcgatgaaca gatcagtccg catgagtgcc tatggttcgg
    16141 tggttctctg gaccatggtg ctgctggtgt ggccggggtg ccgagccgtg tcttcgacgc
    16201 cggatcccgt ccactcgccg catctccaac gggctagagt ctttctcgcg gcgggggatt
    16261 ttcgccgggc tgtcgaagcc tgccgtcagg aggtgtggga acatccatcc gcgagaagct
    16321 atctctatct gacctatgtg tatcaggcac tcgatgccta tgtggagtcg ctggccaaat
    16381 ccgattactg ggtggggatg gagcatctct atttgaactt gacctcgggc agcccggaag
    16441 agcttctcga tcctcccaat gtcctggctc ggatggccaa agagatgatt caagcggcga
    16501 cgcggcagca ggctgacgtc acgtccgagc tggcaaccag gctcgatccg gctgtggttg
    16561 cagtggtatg gccgcaacag actgcatggc gaaaagcgag acccgacgga tggtggttca
    16621 gtgtgcctgc tgaatggcca tggtagcgtc accggcgcca cacgtttgac tccgtcaaga
    16681 gggctggtat acgatgattt gttctggcgg atgcgttcga attgattccg aaggaggatg
    16741 cgacgtggca ggtaacgcgt taaaggtaga ggatgcaacg tgggattccg aagtcatgaa
    16801 ggccgctgag ttggtcatgg tcgacttttg ggctgtctgg tgcggacctt gccagatggt
    16861 cgctccgatt gtcgatgaac tggcgactga atacgccggc aaggtcaagg tccgaaagct
    16921 caatactgat gaaaatccgg aagttgccgg tcgctatcag gtcatgagca ttccgacgat
    16981 tctcttcttc aagaacggcc aggtcgtgga aaagctggtc ggcgccagac cgaaacggca
    17041 attcaaggaa atgattgatc agctgttggc gcaacacgcc ggctccgctt agcaggtcgg
    17101 tgaaaaaggc ctcagcgtcg tgctcgcgtc ggtcaaaggc tcaggaggcg gattcactgg
    17161 gggcaaaata cctaaccgct cgcatttgat cgaagcgagc ggttcaagcg aagtttggta
    17221 tgtacctcct cgcctttttg cgtgccgcgg ccttgctggc ccgccagccc gccttttcgc
    17281 ctaacctgca cggttcctaa ggcaggatcc gctcgattcg gagattacga ctcgccagat
    17341 gcttgccgag ctgcgcattg tcaattttgc cctcatcgaa cagcttcaca tccagtttca
    17401 gtcaggattt accgttctga ccggcgaaac gggcgccggc aaatcgttgc tcatcgatgc
    17461 gattggcttg ttggtgggcg gcagagcctc ggcagagcag attcgttccg gggaagacga
    17521 agcccacctt gaagcggcgt ttcatctacc gctcagccat cccctcctcg cacacatgcg
    17581 ggcgcaggac ctcatcggcg cacgagaaac tgacctgatt ctcaagcgga tcctctcccg
    17641 ttccggtcgc catcgtgtct acgtgaacgg cagcctgtgc ccgctgcgtg tactcgaaga
    17701 cctcggaggt acgctggttg atatccatgg ccagcatgaa cagcaatcat tgctggcttc
    17761 tacccaacaa ttgcatgctg tggatgcctt cgggtcgacg gcaactttgc gcactcaata
    17821 tgagaccgcc tatcgcgaat ggagagattt gacagccgac atcgacgctc tacgctgcgc
    17881 cggggtggag cgagcacgca tggaagagtt gttacggttc caagtccagg agatagagca
    17941 ggccgcattg tccgtcgacg aagaagaccg gctgaacatg gagcgacaac ggctgtcgca
    18001 tgctcaccgc ctccgggagc tagccgaatc gtcttacgcc gtgctccagg aagacgagcg
    18061 ggcgactctg ccacagttgg ccacggtcgc ccgctttgtg tcggaattgg tccaaacaga
    18121 tccgggcatg gaggagtgcg aagtccaggt ccgccaagcg atcatccact tgaaagacgt
    18181 gaccgctcgt ctgcgagggt atgccgatga tcttgaagcg gatcccgcaa gacaagcgac
    18241 cgttgaagac cggttggacc tgattcaccg gctgaagacg aaatatggag gctccaccga
    18301 ggccgtactg gagacgggta agcgtgccag gcaggaattg gagatgctgg agcagcatga
    18361 agcccagatg tccggcatcg tggcgcgact gaaggaggcg gagtctcggg tcattgacaa
    18421 ggcaaagcag ctgacgaaaa aacgccggga agccgccaag cgcctaaccg ccctggtcgc
    18481 gaaggaattg gcagccgtca ggatggcaca taccctgttc gacatcgtgg tgaccgcaga
    18541 cgagcagctc gttcggttcg ggccgaccgg tcgtgatcac ttggaattcc tcttgtccag
    18601 caatgtcggg gagccgcctc gtcctttgaa tcgcgtggcg tcaggcggag aactctcgcg
    18661 catcatgctg gcactgaaaa cggtactcgc cgagacggat caggtgccgg tcctggtctt
    18721 tgatgagatc gataccggtg tcggaggagc ggtggcagct gccatgggta cccggttaag
    18781 aaagctcggt gcctaccatc aggtgttctg tatcactcat ttgccgcagg tggcctccca
    18841 agccgagcac cacgtgattg tggaaaaggg gcaagacggc aggcggacga ccacgtcggt
    18901 gaggttgctc agcggggtgg accgcgaaga ggaaatcgcg cggatgctcg ggggagaaac
    18961 cgtgaccaag aaaattaggg agacggcagc agaactgatc gccggtgcca agagcaagcg
    19021 ctagctgcgc atgtgcgtgc accggctcat gggttgcaag aggtcaccac ttggccacac
    19081 aactgaggtg gtgaaccgag gcgaggccta gacgtccgag cgggtcgagg ggctggtggc
    19141 aatggccatg gccggcaatg aggccttgca ttcctgaaca acctgcgtga acaagccggt
    19201 gagctggggg tcaaagtagc tcccggcgcg agccgaaatg aagctgctcg catcatcgat
    19261 gggaagcgcg atccggtctg gaagttcggc ggtgaggtga tcgaatgttt gggcgagaca
    19321 aacaatcctg gccagaatag gaattccttc tccctttaag ccgtgcgggt agcctgaccc
    19381 gtcccaccgc tcgtggtgat aagcaatgat ctggccagtc gctgcgggta acccgagcgg
    19441 agccaccaac ttcgccccta tttctgggtg ggatcgcagc tcatgagtgg tgttggttgc
    19501 gacgatttcg tcgtccgcaa atcgataggc ttctttcccg accttcccga tgtcatggag
    19561 aaacgcccca agagcaagtg atttctgttc ctccactgtg agattcgtcc gattagccag
    19621 cagcgtggcg taaaaactga cgcgactgca atgatttagg agctggcggt ctttcgcttc
    19681 caggaggtcg gacagcatcg gcagcaaatt gctggcatcg tcatgtgcgg agaggccccg
    19741 tccgctgcat ttgccgatgg cctcatatcc ttcacgcatc tcgtgccagg cctgtgcgca
    19801 ttcccgatcg gtgccccaaa gaccggtgga cgtcgccaaa aaacgacgga ggtagtcgag
    19861 ccgctgcttc ttctcgagtg tctggttgac gagcgtgatg agctccgtga cattgaaggg
    19921 cttgagcagg tagcctgctg ccccgtgtcg gatgccctcc atcgctgact tcaaactacc
    19981 gtagcccgtg atgatgatca cctcaatatt ggcgtgatcc tgtttgatct cttgtaagag
    20041 gtccatccct tgccgatcgg gaagtttctg atcgagggta atcaggtcga tttgctgatg
    20101 gttcaatacg ttaatggccg cttgagccga ttccgctgat tggatattga agaacgggcg
    20161 caggatgact ttcaatgcgt ctcgaggccc ggcttcgtca tcaatgacga ggactgtggg
    20221 cttctgtgcc ggcacgttcc ggagtgcagg gatagtcata ttaaccgctc atgtaccctg
    20281 gaatacgtaa cctgtcaagc aatatatcat ctttttcagc cagttataca gctaacttct
    20341 agtagatgtg gtcgttcgct gccggggcta ccatatattg ttccacgtat gaatgaatac
    20401 ctagagcctc agcgaggcaa gaattggcgg ggtcaatcta ttggagcgga tggacggttt
    20461 gttattcttg cgcaacaggt tccggctcag gaggcgattc accgtcggga cggccgatgc
    20521 ccagcgtatc cattcgatat ttcagcattc gccggctgat accgagttgg ctggccgcgt
    20581 gagtttgcac ataattattc ttttttagcg catccaggat gatttcacgt tcgaattcca
    20641 tgacggcttt ttcgagcgac agacgtccgg ccaaagtatc gtcccgaagg gaggaggatc
    20701 ttgtgtcgtt ccggagaatg gtcgggagat gttccggcgt gaccacgtct gagcccttcg
    20761 accagatgaa cgcctgttcg atgatgtttt ccatttccct gacattgccc ggccatgggt
    20821 accgtgccag gaggtcgata gcctccttgg aaaaatcctg actgggtcgg tgatcctcct
    20881 caatcctttt cgacagaaaa tgcttggcca gcaggggcac gtcctcaccg cgttcgcgca
    20941 ggggcgggag gtacagcgca atcacattga tgcggtagta gagatcttct cggaactggc
    21001 ctttccggac cagttcttcg aggttcttgt tggttgccgc cacgattcgc acgtccactt
    21061 tgatggcctg cacgccgccg atccgcgtaa attcgcgttc ctgcaggact cgcaacaact
    21121 tcgcctgggt cattgcgctg aggtcgccga tttcgtcaag aaatagtgta ccgctgttgg
    21181 ccagttcgaa ctgccccacg cgacgcgccg tggcatccgt gaaggaacct ttttcgtggc
    21241 cgaaaagttc gctttcgatc agggtttccg ggagggcggc gcaattcagt gcgacaaacg
    21301 gacggtctcg tcggccgctg ttgtagtgga gagccttggc caccaattcc ttgccggtgc
    21361 cgctttcgcc ggtaatcaac acggtagtac gactgtcggc gacctgttcg attttgccgt
    21421 agatttcctg c
//
LOCUS       DASSCQ010000220        46204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_16513, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000220 DASSCQ010000000
VERSION     DASSCQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 46204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46204
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16513"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="VFH05_13780"
     CDS             complement(<1..1348)
                     /locus_tag="VFH05_13780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HET6679341.1"
                     /translation="MSKNHKAAQLSRSATVDPAVPDKALSKKPPQQVTAVEMGARQRE
                     ISVSEFFTKNRHLLGFDNPRKALLTCVKEAVDNALDACEEAGILPEVSVKLEVVSNGE
                     AIAPSQASKFRITVTDNGPGIIRQHIPRIFAKLLYGSKFHRLRMSRGQQGIGISAAGM
                     YGQLTTGKPVKIISRTGPRAAAHFFEVQIDTKKNEPLVHENKQIEWDQFQGTQVTLEV
                     EGKYQKGRASVDEWLEQTSIANPHVRLVYHTPEGETKEYSRSYHELPPSPREIKPHPY
                     GIEFGVLLKMLQDTKSHSLSNFLSSDFCRVSPQLAVDICKEAKVSPNLKPRELKGPAA
                     ESLYRTVQTTKIMAPPTNCLSPIGEKAILSGLYKQIKGEFYTAVSRPPAVYRGNPFII
                     EAGLAYGNRPGDQPKPEHATGPKAEGEDEDDNTELARVIRYANRVPLLYQQSACSTF"
     gene            complement(2193..2930)
                     /locus_tag="VFH05_13785"
     CDS             complement(2193..2930)
                     /locus_tag="VFH05_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679342.1"
                     /translation="MKRYSSVQTDLIKSFVEKQTNNPGKLMRILVVFFTLLALCTGNG
                     CVSQGTYDAVVQEGLMTRSELERMQEEHRALVRQAHDLEQMNADSVREAETIVAAVQQ
                     AKDDAEDERHAAEMRIARLKAKAIQAAKQHNALQYELNVAKENTGALQEMIDVYQRKM
                     RDGAPTHPAQGNDPAVHKPFDPSTIPPLQDLPAPAAVEPPKPVAPPLHSPTPAASPTS
                     RTQDPVETGWLDSIKGWLVSLWRSVFS"
     gene            3202..3627
                     /locus_tag="VFH05_13790"
     CDS             3202..3627
                     /locus_tag="VFH05_13790"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET6679343.1"
                     /translation="MGLGTSLIYMVLLGFGSLITPGVGISGRLLEWVPCPLSEFSHAP
                     AYGLLTWLLASGLRGREWPQHVALCVAMPAALVFGIWMEVFQGVVPGRVVEIGDVLMN
                     AMGIGAASLLIGLRLLPIDEASRRWGFRRVLDIKRGCSA"
     gene            3615..3965
                     /locus_tag="VFH05_13795"
     CDS             3615..3965
                     /locus_tag="VFH05_13795"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6679344.1"
                     /translation="MQRVTSHWNNRRCVRTPVSCAVYYSDGQLHASGMMQNLTEHGGC
                     VSGTHGVTVGMELMVLLIRPAKRALMIKKATVRWVRQSHFGVELDEAACGMIGECRDT
                     GNEPPPVPLSIRSH"
     gene            4093..5706
                     /gene="lnt"
                     /locus_tag="VFH05_13800"
     CDS             4093..5706
                     /gene="lnt"
                     /locus_tag="VFH05_13800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET6679345.1"
                     /translation="MSQWPVRLILLAAATGILLPYCFPKYDLGLLAWVALIPLHIALA
                     SSSRRSQAFWLGWLAGIVSSTGIMSWVVTAMNTYGKVPLVISYGIMLLLTAYLGLFVA
                     LYSAGVVWFRMLVPRYGLFAAPCLWVALELLRTYLFTGLPWSLLGYSQYRQLDVIQIA
                     DHLGVYGVSFLIVLTNVALAELYLWLMPLFRGFRPARLPWELVTTSAMLVGLSWAYST
                     SLIVSEGMEQLKTSLRVGVVQPNIDQAVKWDQAYRDETLKRYDRLTESFGYGVDLVVW
                     PEAATPFIYEREPVYQLQLVSMASRASAPLLFGSPAVRFDAERKPFLLNSAYLLSPQG
                     QLLGRYDKQHLVPFGEYIPLKSSLLFFLDKLVEGIGDFQAGPGPIVLSFQPSADSSIS
                     RSVKFGVVICYEVIFPDLVRRMALGGAEFLVTITNDAWFGDSSAPFQHFAMVVFRSVE
                     NHLAFARAANTGISGFIDPFGRIIEASPLFTEAALQATIPVRQTQTFYSRHGDVFAYG
                     CMLISLLLSLYGLFGTKGAEPDAIAATPV"
     gene            5725..6868
                     /gene="prfB"
                     /locus_tag="VFH05_13805"
     CDS             join(5725..5793,5795..6868)
                     /gene="prfB"
                     /locus_tag="VFH05_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET6679346.1"
                     /translation="MLDEIRVRVRAVDERVSELRGHLDLSRMTAQLEELEAKMGQADF
                     WNDARAAAGVSRQKVRLERELQQWRDIEAKLSDVTALLELAQESGDAGLERELASELA
                     QFEPRLAALRVEHLLSGDLDQNNALVAIHPGAGGTESQDWAQMLLRMYVRWAEGKGFK
                     VDTLDLQAGEEAGIKSATVSVAGAYAYGYLKAEAGVHRLVRISPFDANKRRHTSFASV
                     FVYPELNDDIEVAIEEKDLRIDTFRAGGAGGQNVNKVETAIRITHLPTGIVVQCQNER
                     SQLQNRNGAMKILKARLYEQEQKKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTGHEVGQVSAVMDGDIDGFIEAYLKQKLIRGSDQFSPAHESDDV"
     gene            6899..8380
                     /gene="lysS"
                     /locus_tag="VFH05_13810"
     CDS             6899..8380
                     /gene="lysS"
                     /locus_tag="VFH05_13810"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET6679347.1"
                     /translation="MDEANEQRQQRIKKLDQLRAAGVPPYGTRFDAKDRVSDLIKLHG
                     QKTKETLESEKIACTIAGRVVALRRFGKAAFAVLQDGADRLQVYLKKDLLSEKASTVT
                     EQLDLGDWIGVTGTLFRTKTNEFTVEVHDLSFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPEVHGIFAARSRIIAAIRAFLIQRGFLEVETPMMHPIPGGAAAKPFVTHHNA
                     LGVDLYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVAFADY
                     QDLIRLTEELISSLAQQLFGKTVIEYQGQVIDLTPPWRRWSYREAILEVNRLAPAVLT
                     DREQAVAAAKELHVPIDPKWPLFNIVNEIFEETVEPNLQQPTFITDYPMEISPLARRK
                     DADPSLTDRFELYIAGREIANAFSELNDPLDQRERFEGQAAQRAAGDEEAHVVDEDFL
                     RALEYGMPPTAGEGIGIDRLTMLFTNQASIRDVVLFPQLRPEK"
     gene            8385..9662
                     /locus_tag="VFH05_13815"
     CDS             8385..9662
                     /locus_tag="VFH05_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET6679348.1"
                     /translation="MAIPYEIFVGLRYLRAKRRNRTISLNTFVSVTGITLGVAALIGT
                     VGIMTGFKEDIQAKILGTTAHIIVQDRMKDGMSDYDAVAKQLETVPDVVAVTPFILKQ
                     VMLTTQGSAQGVVLRGIDPEREGFVTALAHNMVSGRLEDLSQPIAVKLPPPDNPSGPL
                     QSSEKPGIILGKELALRLGAFVGDTVSVVSPTGTISAMGMVPKIRIFAVAGIFQSGMY
                     EYDSSLAYIDLGEAQKFFSMGETVTGIEIKVIDVFRAGDIARRVEQSLGFSYGARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQKEIAILKAMGATKK
                     SIRRIFMLNGLIIGLSGTAIGIPLGYAFLWLIERLWTFDPTVYYISKIPVHVLAEDVL
                     LVAGSAILISFAATIYPSLQAARLEPVAALRYE"
     gene            9681..10343
                     /locus_tag="VFH05_13820"
     CDS             9681..10343
                     /locus_tag="VFH05_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6679349.1"
                     /translation="MIIVKDLHKSFPMGEQTLTVLRGIDLTIERGELIAVVGASGAGK
                     STLLHILGLLDRPSKGTVCFEGQDLFQLSEAAQAEFRNRRIGFVFQFHHLLPEFTALE
                     NTCMPALIQRREPEEVRQEAIGLLKDVGLGPRLQHKPGELSGGEQQRVAVARALLQKP
                     DLVLADEPTGNLDTHTGDALFALMRELNKARGTTFVIVTHNDKLSAQADRILHMQDGQ
                     II"
     gene            10549..11715
                     /gene="traF"
                     /locus_tag="VFH05_13825"
     CDS             10549..11715
                     /gene="traF"
                     /locus_tag="VFH05_13825"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HET6679350.1"
                     /translation="MRPGLSFGAIIFLALLFVHGKALGAEFVIVGPRAMGMGGAGVAI
                     TTDALATYWNPAGLAMTQTVDIRIVGSAQAIDRLGIADAVGDLENFDTTDTSPANVAK
                     AQDIANRINRPGANTSFNGAAGVYVKGHFGEHAFGFNISNVPTGGGFVSSPVGVSCSP
                     NPNCAAPGPISSVSVSGAMAVRALEARQAAFSYAYAFADKTFAIGVTAKIIQGAAYNG
                     TQNLVGGQEIKLSDSLGEATITTTYGIDVGAIYRPSSWLRFGVVAKDLNQPEFDAPDG
                     TKIKLGPQVRGGMALNPYSSLTLSADVDVTSNKTLVPGVKSQVLSLGAEQTILTEFLS
                     FRLGAFKNMQDAATPFTPTAGLGVRIFMVRLDVGGGYDFREKGALVSGSLSLTF"
     gene            11751..12392
                     /locus_tag="VFH05_13830"
     CDS             11751..12392
                     /locus_tag="VFH05_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679351.1"
                     /translation="MFIRAQHWVRVASCCLTLIIAGCGGLQEVWEGPGAKQFRPQAIA
                     VLPPMASQYDSAREDIQDVLSGALTKTARIERVVTPESVTDIFQGSKEAFDSLVFYFS
                     RLEMTGQSDKESAIKLGKALNVDSFLVVRVNSWEYIRKEGDNVGRVGLSLRLVDATTG
                     TTVWKARHEKSSSYMFYRPNLKDVANDLAKEMIAYMPPQYQNRSSLSQGLASK"
     gene            complement(12419..13234)
                     /gene="trpA"
                     /locus_tag="VFH05_13835"
     CDS             complement(12419..13234)
                     /gene="trpA"
                     /locus_tag="VFH05_13835"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET6679352.1"
                     /translation="MRSSSRLDQTFERLKTKGEKALIAYLMAGDPGLAETEELVLALE
                     KAGADIIELGVPFSDPIADGPVIQQAAERALRSGTSLRKILLMVAAIRTKSQVPLVLM
                     AYYNSIHAYGPDRFCQDATAAGVDGLIVPDMPPDEAGPLRGPACASGLHLIFLLAPTS
                     TPQRRAFVARESKGFVYYVSLTGITGAKLADMADVGANVEKIRELSDTPIAVGFGVAT
                     PQDAARLSRYADGVIVGSAIVRQIAAHQNGGMVSEVSNFVHALKTAMVPGTAP"
     gene            complement(13279..14493)
                     /gene="trpB"
                     /locus_tag="VFH05_13840"
     CDS             complement(13279..14493)
                     /gene="trpB"
                     /locus_tag="VFH05_13840"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET6679353.1"
                     /translation="MNTKLPRPATIPDQQGRFGAYGGRYVPETLMPALLELEREYAQA
                     RKDRAFQQQLAYYLKEYVGRPTPLYFAERLTKRLGGAKIYLKREDLCHTGAHKINNAI
                     GQGLLAKRMKKPRIIAETGAGQHGVATATVAAMFGLQCEIYMGTEDMQRQALNVFRMR
                     LLGSTVTGVDAGSRTLKDAISEAMRDWTTNIRTTHYILGSVLGAHPYPMMIRDFQSII
                     GKEARKQILAAEGRLPDCLVACVGGGSNAIGLFHAFLGDKKVRMIGVEAGGLGVASGK
                     HAARFSGGKPGVLQGTMTYLLQNEDGQINLTHSVSAGLDYAAVGPEHSYLREIGRTTY
                     TSVTDDEALAAFDLLAREEGIVPALESAHAIAHAVQIAPKMQRNRILLINLSGRGDKD
                     VQQVARMRGISL"
     gene            complement(14514..15155)
                     /locus_tag="VFH05_13845"
     CDS             complement(14514..15155)
                     /locus_tag="VFH05_13845"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET6679354.1"
                     /translation="MSKRLKVKICGITNAEDASVAVAAGADALGFIFYEKSPRYIVPA
                     VAARIIAGLPPLVTAVGVFVNEGLATVRSIMDTCGLALAQLHGNEDATYCRELSRPAM
                     KALRLQDRNSFLALAEYQGRAGVRGFVIDTFSEFAYGGTGQVADWALASEAAKTASIL
                     LAGGLTPGNVADAVHAVRPYGVDVSSGVELVPGKKDHEKVRAFIDAVRVVSSS"
     gene            complement(15158..15979)
                     /gene="trpC"
                     /locus_tag="VFH05_13850"
     CDS             complement(15158..15979)
                     /gene="trpC"
                     /locus_tag="VFH05_13850"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET6679355.1"
                     /translation="MILARILEHKKAELRHKQSRGYLADLKAQIRDTSGTLGFSVALD
                     ATRSSVSPALIAEVKKASPSLGLLRPEFVDAFDHVAIAQRYEEAGASALSVLTDKDFF
                     QGSLDYLRAIKRSVPLPVLNKEFMVEEIQFYEARAYGADAVLLIVAALERRQLVDFHA
                     LAGELSLDVLIEVHHERELDTVLEWIPNARMIGINNRDLTTFTTDLTVTKRLAPRIPS
                     DRLIVSESGIQNRGHVEQLLEMGVHAMLIGESLIRADDARTKIQELRGMRSSAQA"
     gene            complement(15976..17004)
                     /gene="trpD"
                     /locus_tag="VFH05_13855"
     CDS             complement(15976..17004)
                     /gene="trpD"
                     /locus_tag="VFH05_13855"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET6679356.1"
                     /translation="MAMIRDAIAKLADRLSLTEKEAEEVMNQIMDGIATPAQISAYLM
                     GLRLKGETVEEIAGSVRAMRARAVRIAVGAPLVIDTCGTGGDGRHTFNISTTTAFVVA
                     GAGLTVAKHGNRSVSSKSGSADVLSELGVNISLSPDLVSECVNEVGIGFLFAPLYHGA
                     MRHCAGPRQEMGIRTMLNLLGPLTNPAGATVQVLGVYEPRLTELLGKVLMHLGSQHCF
                     VVHGMDGLDEITLTERTLVSEAKAGVLSNYVLTPQEFGLNRVSPKELAGGSPQENARI
                     TREILQGKKGAKRDVVCLNAAPALVAGKKAKTLQEAYQLAGDVIDSGAAADKLARLAS
                     FTSKGAHA"
     gene            complement(17013..17576)
                     /locus_tag="VFH05_13860"
     CDS             complement(17013..17576)
                     /locus_tag="VFH05_13860"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET6679357.1"
                     /translation="MLLMIDNYDSFTYNLVQYLGELGEDVRVYRNDKISIEQIESLKP
                     TRIVVSPGPCTPNEAGISVETMKHFAGRLPLLGVCLGHQSLAVAFGGEVVRAMRLMHG
                     KTSPIQHDGRTIFRQLPNPFDATRYHSLIVNRSSLPACFEVSAETAEGEIMGMRHKSL
                     GVEGVQFHPESILTAAGKDLLRNFLKL"
     gene            complement(17659..19155)
                     /gene="trpE"
                     /locus_tag="VFH05_13865"
     CDS             complement(17659..19155)
                     /gene="trpE"
                     /locus_tag="VFH05_13865"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET6679358.1"
                     /translation="MSVPAYSLSLEQFRHYAGQGNLIPLYREILADYETPVSAFAKID
                     HGPSAYLLESIEGGEKWARYSFLGSGSPVVIYEDRGDLIQVRGKRKKRILSRGNPLER
                     LREVMAAYHPVTVPDLPRFVGGAVGYLSYDMVRTFEELPVRQKDHTNLPDFAFLLTDT
                     LLIFDNVSHKIKVVANAHVASTRERDVREAYRAATDRIERMIARLKRPLARTRPHRRR
                     LPISFTSNMNRADFEKMVTRTKEYIRSGDVVQAVLSQRWETNVQASPLQLYRALRVIN
                     PSPYMYYLRVAGLELVGSSPETLVRCEDGVISVRPIAGTRRRGQSTEEDQQFERRLLA
                     DEKERAEHVMLVDLGRNDVGRVARPGSVIVDSLMHVERYSHVMHIVSNVTGQMDSGKT
                     CYDVLRACFPAGTVSGAPKIRAMEIIDELEPTRRGPYAGAVGYFSFSGNMDMCINIRT
                     VVIKKHQAFVQAGAGIVADSNPEHEYEETCNKARAMMKAIELAEQGLE"
     gene            complement(19167..19805)
                     /locus_tag="VFH05_13870"
     CDS             complement(19167..19805)
                     /locus_tag="VFH05_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET6679359.1"
                     /translation="MLEATSVRPSLRPAVRVVALICGGLCLSGCVLSEKYEAEKARSL
                     NFQRLLAQEEKRTGELDNEVKRTKRELAEYETRNRELTAQVQAVREQMSRVQEEAEAI
                     KEASLLERKAKADMKRSAPPAVKAPPVESSPAITELLGTAPNAQSDVAFSTSDAQVEG
                     KGASTVHVVKPGETLYRISRRYGVKVDTLRKWNKLPDDIIEVGQKLVVSQEQ"
     gene            20038..22041
                     /gene="ligA"
                     /locus_tag="VFH05_13875"
     CDS             20038..22041
                     /gene="ligA"
                     /locus_tag="VFH05_13875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET6679360.1"
                     /translation="MGTTDIQARVAALREEIRRHDYLYYVQAKPEISDSAYDRLFREL
                     TELEQAHPELVTADSPTQRVGAPPLDSLSKVRHEQAMLSLDSLVEQSDVLAFDQRMKR
                     ELGVSSVGYTAEPKFDGLSVELLYERGFFTRGATRGDGVTGEDVTVNLKTIRALPLQL
                     MPGTGYPDRLVVRGEVYMKLDEFQAMNRKLTERGEDGFANPRNAASGALRQLDSAITA
                     ARPLALTCYEIMAWSGKGLPTHWDELEALARWGFPIPLHRLKCDSIQEVIAYHRETEH
                     LRDSLPYEIDGVVIKVDRRDWQERLGYKSRSPRYAIAYKFTPRKEITKVKDIVVSVGR
                     TGTLTPLALLTPVEVGGVTISRATLHNADEVARKDIRVGDTVRVERAGDVIPAIAERV
                     PVPDERRGEPFRMPDHCPVCQSSVAREGAYYYCTGQSVCMAQLKGAIEHFASKSALNI
                     AGLGRKTVAQLVDAGLVHSLADLYRLTREQLLALEGFGERSAGLLLEAIERSKRVSLD
                     RFLMGLGIRQVGQHIAKVLAREFGSLTRIIDSDDARFQQVSAIGPEISSSLVSYFSEA
                     HNRRVIQDLLDSGFDILAESVAASARQTLAGKTFVFTGGLKDLTREQAGKLVEERGGR
                     IASTVSKKTAYVVAGADAGSKLEQAAKMGITVLSEEEFRQLVE"
     gene            complement(22052..23335)
                     /locus_tag="VFH05_13880"
     CDS             complement(22052..23335)
                     /locus_tag="VFH05_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6679361.1"
                     /translation="MDIAILLGLIVLSAVISTAEIGFFAVNETRLRALAQNQNSRAKM
                     ALHLRSDPQKLLATILVGDRLVGTAIPMYATFLTLNTYGADRTFFDDTIAVVVGLLTF
                     VLLVSVDVVPKTLAAKFAVPVTLNMAYPVYGVQLVLKPILALMVPFIHRLTGGKGLTL
                     PLVTEEELKIMLDEGGKTGAIEVEKVKMIKNVFQLKDITAEDAMTPRLYVFSLDGNLR
                     LKEAQELLYNSKYSRIPVYDGTLDNITGILYKSRALTELAKNQTDAKLKEIAYPPIFV
                     PAGKTADDLMKQFQQEKRHMAVVVNEFGGVMGIVTLEDLLEEVVGEIMDETDITEELI
                     KRLGKNHILVHGRTEVRKINDFLKVDLGDEAVTISGLIQQELGRIPKVGEELRIGNCR
                     IVIHEAEPRSIRSVQIYREEKTPVAVEAPNLNLVS"
     gene            complement(23349..23483)
                     /locus_tag="VFH05_13885"
     CDS             complement(23349..23483)
                     /locus_tag="VFH05_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679362.1"
                     /translation="MKLLVPPKVFRIDLGISAMCLACPRAGSGFLAPICQATVQVTRP
                     "
     gene            complement(23568..24296)
                     /locus_tag="VFH05_13890"
     CDS             complement(23568..24296)
                     /locus_tag="VFH05_13890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET6679363.1"
                     /translation="MPICPPGSSDGRVCLLTISRGLLSGFRFSPAFVSALVVTATVIG
                     CSSPPKPTYFPGYPIGYVEGGVASWYGPGFHGNRTANGERYDMHKLTAAHRTLPLGSI
                     ATIRSATTGREVTVRINDRGPFAKGRVLDLSMAGAQALGMIGSGTDRIELRVIGFQGR
                     TADMGFLRVQIGSFVEQQNALNLLERARRLYPGGRIQPVDLPEGRRYRVHVGEFKTEH
                     QAESAARRLEADLATQSFVFRDDN"
     gene            complement(24334..24744)
                     /locus_tag="VFH05_13895"
     CDS             complement(24334..24744)
                     /locus_tag="VFH05_13895"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679364.1"
                     /translation="MPTTTQFVISGQSRPGVMAEVASVLAAAGVNIKAFSAPEVTGTG
                     KLRLLVADVEGARTALRGAKIKFTEETALILSLENKPGALNEVADLLTRARINIKCGY
                     CTPSREGKRAIVVLTVANTEKALAILRDQSLDEF"
     gene            complement(24841..25248)
                     /locus_tag="VFH05_13900"
     CDS             complement(24841..25248)
                     /locus_tag="VFH05_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679365.1"
                     /translation="MTTSLYERGLETVHDQVVGLVAQRWAKAFVCKVTIKGSVEHHSW
                     ASPEQSCDIVGWYVSSGGDTMEWMAEVETEASLSDPQIADKWMRTMKRGIPFYLLVPR
                     GLKDSVQKLTQDASLVISCIYEYTVINGVCHIL"
     gene            complement(25306..28845)
                     /locus_tag="VFH05_13905"
     CDS             complement(25306..28845)
                     /locus_tag="VFH05_13905"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET6679366.1"
                     /translation="MRIERRFTRSGQSPYEGLKFVKRSSEIRNPDGSTVFKLDNIDIP
                     ESWSQLAIDILAQKYFRKAGVPQRDDAGNPILGPDGKPALGGERDAKQVFERMAGCWT
                     HWGKTYGYFKTIEDSAAFHDELCFMLAHQMAAPNSPQWFNTGLHYAYGLSGPAQGHFY
                     VDPKTREVVKATNAFEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGSGTGTN
                     FSKLRGDGEALSGGGRSSGLMSFLRIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDIEE
                     FIDWKVVEEQKVAAMVTGSKICAQRLNAVLKACHIVDAEGAPRVSVDPKQNSILREAL
                     AAARRDNVPEAYIQRMFSYAQQGFTHFVFHEYDTNWDGKAYQTVSGQNSNNSVRIPNE
                     FFAALEADGDWQLKRRIDGKVAKTVKARDLWDRVAWAAWICADPGTQYDTTINEWHTC
                     PEDGRINASNPCSEYMFLDDTACNLASLNLAQFFTSEGEFELEHFRHAVRIWTIVLEI
                     SVLMASFPSRSIAEKSYQFRTLGLGYANLGTILMRQGIPYDSDKALAICGALTSIMTG
                     EAYSTSAEMAAELWPFPGYAKNREAMLRVIRNHRRAAYSAPEEEYEGLTIKPTSIHAE
                     HCPPDLLIAARRAWDRALELGTAYGYRNAQVTVIAPTGTIGLVMDCDTTGIEPDFALV
                     KFKKLAGGGYFKIINQSLPPALSTLGYTEAQVQDIVNYCVGRQTLQGAPFINHETLKQ
                     KGFDDAALARMEAGLSQAFEIQFAFNKYVLGEEFCRAKLGLTDVQLIEANFNMLKALG
                     FTQEEIAAANDYCCGAMTVEGAPHLKSDHLPVFDCANRCGRIGQRYIAVDAHIRMMAA
                     AQPFISGAISKTINMPADATLEEVKSAYLFAWKSMVKAVALYRDGSKLSQPLSASSDG
                     GKSLETSTEVLTIAEKITERVLVRYLAKRRPLPARRNGYTQKAVIGGHKLYLRTGEYE
                     DGTVGEIFLDMHKEGAAFRSLMNCFAIAISLGLQHGVPLEEFVEAFVFTRFEPNGPVK
                     LNDRIKMSTSIIDYIFRELAVTYLDRYDLAQVKEEDLRMDSVKKDDQDPECSDEEADP
                     QMLASSSINTEMFPSRRGIQPRHGGNGHGNGNGSVTHKVELKRETLTMTAVQIARQKG
                     YEGDPCSECKQFTMVRNGTCLKCETCGATSGCS"
     gene            complement(29192..29563)
                     /locus_tag="VFH05_13910"
     CDS             complement(29192..29563)
                     /locus_tag="VFH05_13910"
                     /inference="COORDINATES: protein motif:HMM:NF025629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4359 domain-containing protein"
                     /protein_id="HET6679367.1"
                     /translation="MTLFRLGMLVAALSLCVGLALSNPTLDEYLTFVERELTKAIERS
                     DEAQLSPERAMVRTIFRSHSHELVDSVVRPHTVRRNWGLVSIYDSSVFNSRIVVLGVA
                     GRFIPLQGIDEAIVRLGRLAF"
     gene            29665..29964
                     /locus_tag="VFH05_13915"
     CDS             29665..29964
                     /locus_tag="VFH05_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679368.1"
                     /translation="MFVWSKKLIAALLALFLVLLGVFLIHGAPDSASGLKIHTTRWMA
                     LNYIGLMVWVFVWMIDQTRMRGKNVWIWLMPFALAPLPTLMLFVLFLQRRLKPVS"
     gene            complement(29948..31219)
                     /locus_tag="VFH05_13920"
     CDS             complement(29948..31219)
                     /locus_tag="VFH05_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6679369.1"
                     /translation="MAADSFAHILANKRVALMLPLGFASGLPLALTSGTLQAWLTVAG
                     LDLKTIGIFTLIGLPYSLKFLWAPFIDRISLPWLGRRRGWMLVTQIAVALGLALMGLI
                     GSSESPHWLGLIAVGVAFCSASLDIVFDAYRTDLLLPAERGFGAALWVNGYRLAMLVA
                     SAGALVLAEQIGWKGTYLVLAALMGAGIVTILLSPNPPGGGAAPRTLKEAVGFPIREL
                     FARPGIYGLLCLIILYKLGDAIAGSLQTAFFIGGLGFTAGEVGYVKGLGIAATLIGAL
                     AGGLIMARSGLIRSLLFFGLLQAFSNLSFMFLAWIGKSYEALAASVVIENVTGGMGTA
                     AFVALVMSLCDHRYTATQFALLSSLEALGRVFAGRPSAEAVGILGWDWFFFLSFVLAL
                     PGCWLVWLYRRQLPQDLSDETSPDSPVMKPA"
     gene            complement(31339..31755)
                     /locus_tag="VFH05_13925"
     CDS             complement(31339..31755)
                     /locus_tag="VFH05_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679370.1"
                     /translation="MRTPVMIRQFTACCVISLSILVQEHAWSKDVLPIEPDLSTRIDE
                     LYDHEARLFLMLYSLKGDGQIDYVTGRMVQEYARSNFGNPVYQTEIHPLFYWWNHNMW
                     NDPEQDGVNGNERIYQENIEFDVSRYKPCTFNGQPC"
     gene            31871..33172
                     /gene="mtaB"
                     /locus_tag="VFH05_13930"
     CDS             31871..33172
                     /gene="mtaB"
                     /locus_tag="VFH05_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HET6679371.1"
                     /translation="MIKRASLHSLGCRLNQAETSVLASQLRRQGYALVDFGEPTDLLV
                     VNTCSVTEEAERNCRYAIRKTLKHSPDAFVAVTGCYAQTGLQSLRSIPGIDLIVGNQF
                     KSDLPSFLPPPQILRKQPAPEVLHTKSIDRHDFVLPEFGESHSTRALVKIQDGCNVMC
                     SFCLIPFARGRERSRRVDDILSEATALAAAGHHELVLTGVNIGQFSQGGVTLVGLIDR
                     LEGIQGIERIRLSSIEPTTVTDDLLERMGASRKLCPYLHVPLQSGDDAILNAMNRPYD
                     VKTFISLVERATARVPDLTWGTDVMVGFPGESDRAFERTVDVCRQLPFSYFHVFTYSR
                     RPGTAAARMAGTIPVNVARLRAKTLADLSRDKRFAFAERWIGSSVAVLFESGTTEGFR
                     LGTTRHFLRVAVSSETQLANQIHPVIITGASDRWAVGHIHN"
     gene            33204..34592
                     /gene="miaB"
                     /locus_tag="VFH05_13935"
     CDS             33204..34592
                     /gene="miaB"
                     /locus_tag="VFH05_13935"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET6679372.1"
                     /translation="MRQVHIETFGCQMNEYDTELVRALLKKAGYGFTEDRECADVILM
                     NTCAIRENAHTKVYTHLSDLRALKKSRPLVVGVLGCMAQNLKAELTKQEPMVDLLAGP
                     DSYRQLPMLIDHAMQAQEVGVHMQHLAVDLSEYETYEGIVPARLSGVNAWIAVMRGCD
                     NFCSFCVVPYTRGRERSREPDGILEEARRIAAEGFKQITLLGQNVNSYRSGEWDFARL
                     LAAVADVPGIERVRFTSPHPKDFPPALLEAVAFHPKICKHIHLPVQSGNDRILNLMNR
                     SYTRREYLTLVEQIRSINPDIVLTTDIIAGFCTETEGEFADTYRLLEEVRYHSAFIFV
                     YSERANTIAARKFPDDVAPEVKSQRVTALVEMQRRISSERNREYVNRTLPVLVEGDAK
                     KSRLQAMGKTDGHITVIWNKCDLPSRPGQVVSCSIYDASASTLYAKAPTFSDLHRPEN
                     EHSCTNSDQGVA"
     gene            34879..35016
                     /locus_tag="VFH05_13940"
     CDS             34879..35016
                     /locus_tag="VFH05_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679373.1"
                     /translation="MNSSSNQTENAQERETPTLSLEAIIAMGLFGWLALSMTLMGLGI
                     W"
     gene            35291..35992
                     /locus_tag="VFH05_13945"
     CDS             35291..35992
                     /locus_tag="VFH05_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679374.1"
                     /translation="MKGVHSSNAQQGSMRMSAGGSSKPMRVFSDEDAEFLAPLLAQGL
                     SKAKPEQLVGFRVSSSAGSGSEPTAGSIYVRQDAIHFTVSRGAGPTIFLPESVARSEQ
                     APAYATGGKTGAVAHVIDYHMLARAPMPALPAMKTSAAQTASSPVTAPGVMTSATAVS
                     AYEGIENGSSVETQTQLRRAKDMLTKKDTEINMLRKESTWMKRELRERDDEIKALKAT
                     VKKTPKKKAEAYPTH"
     gene            complement(35997..36704)
                     /locus_tag="VFH05_13950"
     CDS             complement(35997..36704)
                     /locus_tag="VFH05_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET6679375.1"
                     /translation="MKVVLDIETIQSTREDWARLAGKCYSPIGEEPEYDLFAAGEAEA
                     QRQAENEQYAKSAFDGTYSSIVCIGLLEFSDQMEPRGAVAWYGNNERELLRQFWSRLA
                     QDKPSLFITHNGLGFDLPFIRKRSIIHQVKPSMEINLAKFRSEPVYDTMAIWSNWDMR
                     CWVKLDVLARALNVETKSGSGAQVAEMWGRGEGVELARYCLQDTYVTYACYCRMNFRQ
                     PLSGEVVLLQPELLDVG"
     gene            complement(36701..37108)
                     /locus_tag="VFH05_13955"
     CDS             complement(36701..37108)
                     /locus_tag="VFH05_13955"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HET6679376.1"
                     /translation="MTNVVEVAAGLIAHEGRYLIAKRRTGVHLAGLWEFPGGKREPGE
                     TLEECLQRELFEELSIRIDGPIPFRIIRHEYPEKTVELHFFRCTIGEGRATPLDCAEI
                     RWVHPSEMGQYEFPPADAPVIEALQRDSMGQNT"
     gene            complement(37108..37728)
                     /locus_tag="VFH05_13960"
     CDS             complement(37108..37728)
                     /locus_tag="VFH05_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET6679377.1"
                     /translation="MKWYASHGRDLPWRKTSDPYHILVSEVMLQQTQVDRVVPKYLQF
                     LDRYPSLQELAQAPVNQVKQIWYPLGYNIRPERLHSIACETVERYGGILPNDHEQLLS
                     FKGIGRYTAGAIRSFAFNEDAPILDTNVMRVLHRVFIGRGNPLRQKVKLWKLAEALIP
                     PGRGYDFNQAIMDFGALCCTARTPSCQGCTMKPICRTFPFTPRGKA"
     gene            37974..38465
                     /locus_tag="VFH05_13965"
     CDS             37974..38465
                     /locus_tag="VFH05_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679378.1"
                     /translation="MTFTASRSPWQPLLVFSVLLAGVDLLSVNNRATASPQQVPWECS
                     NYTDEAQTRCLSAFIERQQEQIGKLESQLQNQQDMVGQLKGQVERQSAATAQLQQQLA
                     QPPVTTTIVPPPYLYPYAFAYPPVGIGLYLGRPWIGPGYYGYYGRPWGPRFYYYGHHG
                     RRR"
     gene            complement(38484..39203)
                     /locus_tag="VFH05_13970"
     CDS             complement(38484..39203)
                     /locus_tag="VFH05_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6679379.1"
                     /translation="MSRLTGKVAIVTGSSSGIGKAIALRFGEEGASVIVAARNAEGCE
                     KTARAIRQKGGDARVIQTDIRHEGQVDALFERTVQDCGRVDVAVHNAGIFGGNRIAET
                     STEAFDEVIRVNLRGTFLCCRAAFKQMKKQGGGLIINVSSVAGVQAWAGTGSYSASKH
                     GVMALTRALADEGRPFHIKVSAICPGGVAEDLVDASESDILQSEKISPFDIAETAVYL
                     CSLSRFAVVPEVVVNRLGADW"
     gene            39391..39597
                     /locus_tag="VFH05_13975"
     CDS             39391..39597
                     /locus_tag="VFH05_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679380.1"
                     /translation="MSVATAIVYNPGMGGCKIVTNQALIVGGMQDVPGPLVPPLRLTA
                     RIPGMELDRKRLAQLLQLAAVPLK"
     gene            39611..39937
                     /locus_tag="VFH05_13980"
     CDS             39611..39937
                     /locus_tag="VFH05_13980"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6679381.1"
                     /translation="MVTRKHRRFVVTLPSTLVQRDQFRHKGSVRDLSAKGCRVESLIS
                     PFTGMQVSLLLQLPGESQPVRIENAAVRWCGSQGIGIEFLVVAEPEQARLGHFIQQLE
                     KTAPGN"
     gene            complement(40463..40885)
                     /locus_tag="VFH05_13985"
     CDS             complement(40463..40885)
                     /locus_tag="VFH05_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6679382.1"
                     /translation="MAGCIAEWNGRILLCRRAIEPKSGLWTYPAGFMEIGEGTEEAAR
                     RETFEEARAKVRITRLHSVLSLPHIGQVYLTFIGELLAEEFAAGEESLDARLFEKADI
                     PWQDLAFPVIKDALRRYIEDVRRGEFGVHVADLPGRLS"
     gene            complement(40913..41002)
                     /locus_tag="VFH05_13990"
                     /pseudo
     CDS             complement(40913..41002)
                     /locus_tag="VFH05_13990"
                     /inference="COORDINATES: protein motif:HMM:NF026153.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            41119..42210
                     /locus_tag="VFH05_13995"
     CDS             41119..42210
                     /locus_tag="VFH05_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HET6679383.1"
                     /translation="MSATIATHGGVGFHAAWSTVYRINLRSHIASRVLWEISHETYGT
                     EEDVYRAAYRLNWPEWFASSRTIKVKVTAQRCSFRSLDFLTLRIKDAVCDKFVALCGK
                     RPSVDRRQPDIRIDVFLSSDGVTFYLDTSGEPLFKRGYRQATAEAPLRENLAAGVLRL
                     AGWTPDEVLMDPMCGSGTIPLEAALMARRRAPGFGRSFAFEYLRSYDALQWADAREAA
                     KTERRTEVPLGIYASDHNVAAIELAARTFQVAGVASDIQLRQSDVLSIDPPADEGLLV
                     INPPYGVRLGDRTDLRAFYPQLGDCLKQRFVGWRAYVLTADFLFAKEIGLSPSRKIPL
                     YNGPIECRLYEFKIVRGRPKRSRKSPSIT"
     gene            42229..44295
                     /locus_tag="VFH05_14000"
     CDS             42229..44295
                     /locus_tag="VFH05_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679384.1"
                     /translation="MAMIKYNSSDLLPSKTVIRLLLSVSVLVLLALPGCSLVQAFFAS
                     LGVPEGAVTNTPMRDSSVMRLEPIVRPMFDRLLQVPQAELIEAHSTVETMSARYKRHL
                     TLTALKGPWEGFINVERQGLLLAELAEGRAVNLPALLDVLEAGMDRTSAFNRPISIPA
                     KATAQELITFMIESLEEASIHREKALASLPEDERRFLFSHAQTIVEQFTPQISSVSAT
                     TIAQAKMDQRFAELLEEQVDYANLMAAAQVLARLANESWLRQLAGAFGQALPRSEVPV
                     GITGDILLAQATSYGMIVIGGPGPNTYELDHRFALIVDLGGDDLYRGLIAASADIEHG
                     NAVIIDMSGNDTYDSAALGLATGRLGVGLLIDQAGDDVYQLEIGSGGAGFGGLGILFD
                     AKGNDLYMGTRLTQGAAIGGLGLLFDAAGNDRYASHGFALGFGGPQGVGAAIDLQGDD
                     EYQCGNKYPSAYNEEDAPNGKPGDPMFQYDCFGLGTGAGRRLLTKRQEWQAYDLAGGW
                     GLLLDVEGNDRYRSANFSQGHGYFFGVGAFLDLSGNDEYVAARYGHGSSAHYGVGFFT
                     DRQGADHYESTGPFYNGGVAWDHGMSVMIDAGIESDRYGFQSSNGLGKADYSGWGLFI
                     DEGGNDSYQTKDGYGLSSQHGIGGFFDLKGTDTYQMAPSTSDADLRPTDGKVLLYPSG
                     GLFVDR"
     gene            44404..>46204
                     /gene="lon"
                     /locus_tag="VFH05_14005"
     CDS             44404..>46204
                     /gene="lon"
                     /locus_tag="VFH05_14005"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6679385.1"
                     /translation="MNSTNLSVTLPVLPIKRTVLFPGILMPVTVGRERSVAAVNAALK
                     TEDKMILVVAQRDPAKENPGLDDLYTIGTKAIVKQVGRAEDGVIQALIQGIERVALLK
                     VEQADPYLMVQARRLELPTDNDREVQALQRAIQDSITDLPRLVQAPGIEEATAAFRNE
                     ENPATLAYRVASLLNLTVKEEQTLLESSSTIELLRSLYAFLSKEIQILQLREKINSDT
                     QAKIGKNQREYVLREQLKAIQQELGEGDAEESEVAHLKKRIQEADLPEHVRKEVEREI
                     ARLSKVPPSSPDHQVLRTYLELVLELPWTKASEEHMDLTNVRQVLEEDHYGIKEVKER
                     IVEHLAVLKLNPTAKAPILCLVGPPGVGKTSLGQSIARAMGRRFERFSLGGVHDEAEL
                     RGHRRTYVGALPGRIIQAMRRAGVNNPVLMLDEVDKLGRDFRGDPAAALLEILDPAQN
                     HTFRDHYLDLPFDLSKVFFITTANTLDTISQPLLDRMEIIRVQGYSEREKAEIARRYL
                     WPRRLKEAGLEADHVQLPDGVLDRIISRYTREAGVRQLEQMLGRLTRKIALTVAELPE
                     SHVRETVAVEEDKLPDWLGSERFMPEEARKHIPV"
BASE COUNT         9844 a        12960 c        13004 g        10396 t
ORIGIN      
        1 tgaatgtgga acaggcggat tgctggtaca gtaaggggac ccgattggcg taacggatga
       61 ctcgtgcgag ctcggtgtta tcgtcctcgt cctcaccctc ggcctttggt cccgtcgcgt
      121 gttccggttt gggctgatca cccggtcggt tcccataggc caagccagcc tcgatgatga
      181 agggattgcc gcggtagacg gccggcggcc ggctgacggc ggtatagaac tcacctttaa
      241 tttgtttgta gaggcctgac aggatggctt tctccccgat cggagaaaga caattggttg
      301 ggggggccat aattttcgtg gtttggactg tccggtacaa tgattcggct gcgggtccct
      361 tcagttcacg aggcttgaga ttcggggaaa ccttcgcctc cttgcagatg tccactgcca
      421 gttgaggcga gacccggcag aaatcactgg agagaaagtt ggacaatgag tggctcttcg
      481 tgtcctgcaa cattttgagt agcacgccga attcaattcc gtagggatga ggtttgattt
      541 cgcgcggcga gggcggcagc tcatgatacg aacgggaata ttccttcgtt tcgccttcgg
      601 gtgtgtggta cacgagtcgg acgtgaggat tcgcaatgga cgtctgttcc aaccattcgt
      661 cgactgaagc ccggcctttc tgatatttgc cttcaacttc aagcgtgact tgcgtgcctt
      721 ggaactggtc ccattcgatc tgtttgttct cgtgcacaag cggttcattt ttctttgtat
      781 cgatctggac ttcgaagaag tgagcggctg ctcgcggccc ggtccgggaa atgattttga
      841 ctggttttcc ggtcgtgagc tgaccataca tgccggccgc agaaatgccg atcccttgtt
      901 ggcctcggct catacgaagg cgatgaaatt ttgagccgta aagcaatttc gcaaaaatcc
      961 ttggaatatg ctgtcgtatg atgccagggc cgttgtccgt cacggtgata cggaatttgc
     1021 tggcctggct cggcgcaatc gcctctccgt tggagaccac ttcaagtttg acgctgacct
     1081 ccggcaagat cccggcctct tcgcaggcgt ccaaggcatt atccactgcc tccttgacgc
     1141 acgtgaggag ggccttccgc ggattatcaa aaccgaggag gtgccggttc ttcgtgaaaa
     1201 attccgaaac ggagatttct cgctgccgtg cgcccatttc caccgccgtc acttgttgcg
     1261 gcggtttttt tgaaagggct ttgtcaggaa cggcaggatc gactgttgcg gatcgagaaa
     1321 gttgagcggc cttgtggttc ttcgacattc cacctcgtat ggtatgggga gagtgcgtct
     1381 tggtatcaca aacgatagcg cgaggcaacg gtgattcgtc ggccgtcgat ttcctgctcc
     1441 atgtatttca tgcaccatgt agattgagac gctcgcgcga tacattagtt tgcttgacga
     1501 gcttcgagga gaagcctatc gttatttgtt gtgggaggag ttaacgccca gagggaatgt
     1561 ggtctatcag gggaccgagc gctgaggatg gtgtgcaggc ccagcttgta ctgggaagcc
     1621 taaagtcctc ccaaggtctc caccgtacct atcactctcg gtaggtgcgt gctcctccca
     1681 ccccaaacac tcgttgccaa tcagacttcc gaatcatccc tcccagacat cgacgtgtac
     1741 ctcagagtcg ctcgtgttcc tcctgctgga tgcatggatc tatcaaaaat ctgccatttt
     1801 accgatcccc attttctgtg gataagtctg tgaacaactt ccttcttgga tcaggaacag
     1861 tcgtgtaact cgcatcggca cagcgaactg cctaattttt aggcataggc ctgtttgtca
     1921 tctcaccacc actaatggtg tggaataaaa tcggcaagtc aattgctgta ccagcagaga
     1981 gatgtggacg tttcacccgc tgtagaagcg ctttttatgc ttggggtccg gtaaatatcc
     2041 acagggaaga ggcttatctc tttctcgaag cagagatctc aagcctgcca agacgtttcc
     2101 caattgcgca ttcattgctc ggattcctga ctggcaacgg catctaggaa aatcacaaga
     2161 caggcaaaaa aactgaaaga gaggcaggag ggttaagaga agacggagcg ccagagagaa
     2221 acgagccaac ccttgatact gtcaagccaa cctgtctcga ctggatcctg ggtacgcgag
     2281 gtagggcttg cggccggcgt gggcgagtga agcggcgggg caacaggttt cgggggttcc
     2341 actgccgcag gcgccggtag atcctgtagc ggtggaatcg ttgaaggatc gaatggtttg
     2401 tgaaccgcgg ggtcgtttcc ctgagccggg tgagtcggag ctccatcccg catcttccgc
     2461 tgatatacgt caatcatttc ctgcagtgcg ccggtatttt ccttggccac attcaactcg
     2521 tactgaaggg cgttgtgctg tttggcggct tgaatggctt tcgctttcaa cctcgcgata
     2581 cgcatttcag cagcgtgacg ttcgtcttct gcatcgtctt tggcttgctg tacggctgcc
     2641 acgatggttt cggcctcgcg cacgctgtcc gcgttcatct gttcaaggtc atgtgcttgc
     2701 cggacgaggg ctctgtgctc ctcctgcatc cgctcgagtt cagacctcgt catgagtcct
     2761 tcctgcacga cggcatcgta ggtgccttgg ctgacacagc cgttgcccgt acagagagcg
     2821 agcagtgtga agaagacgac gaggatgcgc atcagcttac ccgggttgtt ggtctgtttt
     2881 tcaacgaacg acttgatcag atctgtctga acggaagaat acctcttcac tcgtgagaaa
     2941 tcaccaactt gatgtgttcg tgctctcccc cgaaagatgg cagaactgct tggccggcaa
     3001 cagtcaagat caaccggagg aagtgcaaag tctaagccca tgattatcca gatactttgc
     3061 ttcaattttc atctgtgcga aaacccgtaa agctcgattc ctcttccatg cgatcccctc
     3121 tctcacgccc taccattgcc cagtagtttc tcagtaggtg atcttgtagg ttcctctcac
     3181 gtgcgttgat tggagtcatg catgggcttg ggtacgtcac tgatctatat ggtgctcttg
     3241 ggattcggct ctttgatcac gccgggagta ggaatttccg gccgtttatt ggaatgggtc
     3301 ccttgtcccc tttcggaatt ttcgcatgcg ccggcctatg ggctcttaac gtggttgctc
     3361 gccagtgggt tgcgaggtcg agaatggcct cagcatgttg ccttgtgcgt ggctatgccg
     3421 gcggcgctgg tgttcgggat atggatggaa gtgttccaag gcgtcgtgcc gggtcgagtc
     3481 gtggaaatcg gtgatgttct gatgaatgcg atggggattg gagcggcatc gctcctgatc
     3541 ggactgcggc tgctgcctat cgatgaagca agccgtcgct gggggtttcg tcgagtcctg
     3601 gacatcaaga gaggatgcag cgcgtgactt ctcattggaa caaccgacgc tgcgtccgca
     3661 ctcctgtgtc atgcgccgtc tattattcag atgggcagtt acatgcaagc ggtatgatgc
     3721 aaaacctcac agagcatggc ggctgtgtct cgggcaccca tggcgtcacc gtgggtatgg
     3781 aactcatggt cctcctcatt cgtcctgcga agcgtgcgct gatgatcaag aaagcaaccg
     3841 tacgttgggt gcgtcagtca catttcggtg tcgagctgga tgaagcggct tgcggaatga
     3901 tcggtgagtg ccgcgatacc ggtaatgagc ccccaccggt ccccctctcg atccggagcc
     3961 attagccgct catcccccta cggtcgcgcc gccacacagt gtcgcacttt tcgtcgggca
     4021 gagacccggc tccatcgggc cttctgccca agttggttgc aacagccttt atcgttccgc
     4081 taagttagcg tgatgagtca gtggcccgtc cgccttatcc tgctcgccgc cgcgacgggc
     4141 attctgcttc cctactgctt tcccaaatac gatctcggcc ttctggcctg ggtcgcgctg
     4201 attccccttc atatcgcctt ggccagcagc agtcgacgct cacaggcgtt ttggttgggc
     4261 tggctcgccg gcatcgtcag ttccaccggc atcatgtcct gggtcgtcac ggcgatgaat
     4321 acctacggca aagtcccctt ggtgattagc tatgggatta tgctgttgtt gacggcgtat
     4381 ctgggtcttt tcgtcgccct ctatagtgcc ggtgttgtat ggttccgaat gctggtcccc
     4441 cgctacggcc tattcgccgc accctgtttg tgggtggcgc tggaattgct gcgcacctat
     4501 ctcttcacag ggttgccctg gtcgcttctc gggtattcac agtatcgtca gctcgacgtg
     4561 attcagatcg ccgatcattt gggagtctat ggggtgtcgt ttctgattgt gctgaccaat
     4621 gtcgccctgg cggaattgta tctgtggctg atgcccctct ttcgcggatt caggccggcg
     4681 cgtcttccat gggaattagt gacgacctcg gcaatgctgg tgggcctgtc ctgggcctat
     4741 agtacctcat tgatcgtcag cgaggggatg gagcagctca agacctcatt gcgagtcggc
     4801 gtggtgcaac cgaacattga ccaggcggtg aaatgggatc aagcctaccg ggatgagacg
     4861 cttaaacgtt atgaccgact gacagaatcg ttcggctacg gcgtcgatct cgtggtatgg
     4921 ccggaggcgg cgaccccctt catctacgaa cgcgagccgg tgtatcagct gcaactcgtg
     4981 tccatggcca gccgtgcttc agccccgctt ctcttcggca gtcctgccgt gcggtttgat
     5041 gcggaacgca agccgttctt gctgaacagc gcctatctgt tatcgcccca gggccaactg
     5101 cttggccgtt atgacaagca gcacctggtt cccttcggcg agtatattcc gttgaaatcg
     5161 tccctgctgt tctttctcga caagctggtg gaaggcatcg gcgattttca ggccgggccg
     5221 ggtccgatcg tgctgtcgtt ccaaccttcc gcggactcga gcatctcccg atccgtcaag
     5281 ttcggcgtcg tcatttgtta tgaagtcatc tttcccgacc ttgtccgccg gatggcgctg
     5341 ggcggcgccg agtttctcgt gacgatcacg aacgatgcct ggttcggaga ctcgtccgcg
     5401 cccttccagc atttcgcgat ggtcgtgttc cggtcagtcg aaaatcatct ggccttcgcg
     5461 agggccgcaa acaccggcat ttcgggtttc atcgaccctt tcgggcgcat catcgaagcc
     5521 tctccccttt ttaccgaagc tgccctgcag gcgacgattc cggttcggca aacgcagacc
     5581 ttttacagcc gccatggaga tgtctttgcc tacggctgta tgttaatctc cctgctgctc
     5641 tcgctgtacg gcttgtttgg caccaaggga gccgaaccgg acgcgatcgc ggcgaccccc
     5701 gtctaaagga aaggattgtt gttcatgctg gacgaaattc gtgttcgcgt acgtgcggtc
     5761 gacgaacgag tatcggaact tcgggggcat ctttgacctc tcccgcatga ccgcccaact
     5821 ggaagaactc gaagccaaga tgggccaggc tgatttttgg aacgacgcgc gcgcggcggc
     5881 cggcgtgagc cgtcaaaaag tcaggctgga acgcgaactg cagcaatggc gcgacatcga
     5941 ggcgaagctc agcgacgtga cggcactcct ggaattggcg caggaaagtg gcgatgccgg
     6001 tctggagcgg gaactcgcct ctgagctcgc ccaattcgaa ccccgtctgg ccgcacttcg
     6061 tgtggagcat ttgctgtccg gcgacctcga tcagaacaat gcgctcgtcg ccatacatcc
     6121 gggcgcaggt ggcacggagt cacaggactg ggctcaaatg ctgctgcgca tgtacgtccg
     6181 ctgggcagag ggcaaagggt tcaaagtgga cacgctggat ttgcaggcgg gcgaagaagc
     6241 cggaatcaag agcgcgacgg tgtccgttgc cggtgcctat gcgtacggct atttgaaagc
     6301 ggaagccggc gtccatcggc tcgtacgcat atcgccgttc gatgccaaca agcgacggca
     6361 cacgtccttt gcctccgtgt ttgtctatcc cgagcttaac gatgacattg aagtggcgat
     6421 cgaggagaag gatctgagaa tcgatacgtt tcgggcgggc ggcgcgggcg gccagaatgt
     6481 gaacaaagtc gagacggcga tcaggatcac ccatttgccg accggcatcg tcgttcagtg
     6541 tcagaacgag cggtctcaac tccagaaccg caacggggcg atgaaaatcc tgaaagcccg
     6601 gctgtacgag caggaacaga aaaaaaagga agccgagttc aacgccattg tcggcgagaa
     6661 aaaagatatc gcgtggggga gtcaaatccg ttcttacgtg ttccagccct atcagatggt
     6721 caaggaccat cggaccggcc acgaggtcgg acaagtgtcc gcagtcatgg acggcgatat
     6781 tgacggcttc attgaagcct atctgaagca gaagctcatc agagggagcg atcaattctc
     6841 tccggcacac gaatccgatg acgtatagtt gcggcgtagc ggtacgacag ggttgagcat
     6901 ggatgaagcg aacgagcagc ggcagcagcg gatcaaaaag ctcgatcagc tgcgtgcggc
     6961 gggcgtgccc ccctacggaa cacgcttcga tgcgaaggat cgggtgagcg acctcatcaa
     7021 gcttcatggg caaaagacga aagagacgct ggagagcgag aagatcgcct gtaccatcgc
     7081 tggtcgcgtt gtcgcattgc gtcgattcgg caaggcggcc ttcgccgtgc tccaggacgg
     7141 ggccgaccgc ttgcaggtct atttgaagaa ggatctactg tcggaaaagg cctctacagt
     7201 gaccgaacaa ttggacctcg gcgattggat cggggtaaca gggaccttgt ttcgaacgaa
     7261 gaccaacgag ttcaccgtcg aggtccacga cctctcgttt ctcagcaagg cgcttcgtcc
     7321 tctgccggag aaatggcacg gcttgacgga tgtcgaaacc cggtatcgtc agcgttatgt
     7381 cgatttgatc gcaaacccgg aggtgcacgg cattttcgcg gccaggagcc gcattatcgc
     7441 ggccatcaga gcgttcttga ttcaacgggg gtttcttgaa gtcgagacgc cgatgatgca
     7501 cccgattccc ggcggcgctg ccgcgaagcc ttttgtcacc catcacaacg cgctgggcgt
     7561 cgatctctat cttcgaatcg cgccggaatt atatttaaag cggctcatcg tcggcggttt
     7621 tccgcgtgtc ttcgaaatca acaggaactt cagaaacgaa ggcatttcga ccattcacaa
     7681 tcccgaattc acgatgcttg aattctatgt ggcctttgcc gactaccagg acctcattcg
     7741 tctcaccgag gaactgattt catcgttggc ccaacaactg ttcggtaaga ccgtcattga
     7801 gtaccagggc caagtgatcg atttgactcc tccgtggcgg cgttggtcat atcgtgaagc
     7861 tattctggag gtgaaccggc tggctccggc cgtgttgacg gatcgtgagc aggcggtggc
     7921 tgcagccaag gaactacacg tgccgatcga cccgaaatgg ccactgttca acatcgtgaa
     7981 tgagattttt gaagaaaccg tcgagccgaa tctgcagcag ccgactttta ttaccgatta
     8041 tccgatggag atctctccgc tggccaggcg gaaggatgcc gacccctctc tcaccgatcg
     8101 attcgagctc tacattgcgg ggcgggagat tgccaatgcg ttttcggagc tgaacgatcc
     8161 gttggatcaa cgggagcgct tcgagggaca agcggcacag cgcgccgccg gtgacgagga
     8221 agcgcacgta gtcgatgagg atttccttcg ggccttggaa tacggaatgc cgccgacggc
     8281 gggagagggc atcggtattg accgtctcac catgctcttc accaatcaag cttcgatccg
     8341 cgatgtggtg ctctttccgc agctgcgtcc ggagaaataa cctcttggca atcccgtacg
     8401 agatcttcgt cggtcttcgc tacttgcgcg ccaagcggcg gaatcgcacc atttcattga
     8461 atacgttcgt ttcggtgacg ggaatcacct tgggcgtggc tgcactaatc ggcaccgtcg
     8521 gaatcatgac cggcttcaaa gaagacattc aggccaaaat actcggcacg accgctcaca
     8581 tcatcgttca ggatcgcatg aaggacggca tgagcgatta cgatgcggtc gccaaacagc
     8641 tggaaaccgt tcctgacgtc gtggcggtga cgccgttcat cctcaaacag gtgatgctca
     8701 cgactcaagg cagcgctcaa ggcgtcgtcc ttcgcggcat cgatcccgaa cgcgaaggtt
     8761 tcgtgacggc gctcgcccat aatatggtat cgggacgatt ggaagacctg agtcaaccga
     8821 ttgccgtgaa gctgcctccg cccgacaacc cgtctggtcc cctccaatct tctgaaaaac
     8881 ccggcatcat tttgggcaaa gagctggcac ttcgacttgg cgcgtttgtg ggggatacgg
     8941 tcagtgtcgt gtcgccgacg ggcacgatca gcgcgatggg catggttcca aagatcagaa
     9001 ttttcgcggt ggcaggcatt ttccaatccg ggatgtatga atacgactcc tccctcgcct
     9061 atatcgacct cggggaagcg cagaagtttt tcagcatggg agagactgtc acgggcatcg
     9121 aaatcaaagt gatcgatgtc tttcgggccg gcgacatcgc ccgccgcgtg gagcaatcgc
     9181 tcggcttttc ctacggcgcc agagactgga tgcagatgaa ccgtaatttg ttttccgccc
     9241 tcaagctcga aaagacgatg atgttccttc tgctcgtgct catcacgatt gtcgcctctt
     9301 ttaatatcgt gagtaccctg acgatgatcg tcacagaaaa acagaaggag atcgccattt
     9361 tgaaggccat gggcgcgacc aaaaagagca tccgacggat tttcatgctc aacggattga
     9421 ttatcggtct ttcgggcacg gcgatcggca ttccgctggg atatgccttt ttgtggctca
     9481 tcgaacgctt atggaccttt gatccgacgg tctattacat ctcaaaaatc cccgtccacg
     9541 tcttggccga ggacgtgttg ttggttgccg gctccgccat tctcatcagt tttgccgcga
     9601 ccatctatcc ctcgctgcag gccgcaagac tcgaaccggt cgccgccctt cgctatgaat
     9661 agctgaggga cgttgtgagc atgatcatcg tcaaagacct tcacaaatcc tttcccatgg
     9721 gcgaacaaac cttgacggtg ctcagaggga tcgatctgac cattgagcgc ggcgaactca
     9781 ttgccgttgt cggcgcatcc ggcgccggga aaagcactct tctgcacatt ctcgggctgc
     9841 tcgaccgtcc gagcaaaggc acggtgtgtt tcgagggcca ggatttgttt caactgtccg
     9901 aggcggcgca ggccgaattt cgcaacagac ggatcgggtt tgtctttcag tttcatcacc
     9961 tgctgccgga atttaccgcc ttggaaaata cctgcatgcc cgccttgatc cagcgccggg
    10021 agcccgagga agtgcgacag gaggccattg gtctcttgaa ggacgtggga ttgggtccgc
    10081 gcctgcaaca caagccgggc gaattatccg gaggggaaca gcagcgggtg gccgtggccc
    10141 gtgccctgct gcagaagcct gacctcgtgt tggccgatga gccgaccggc aatttggaca
    10201 ctcacaccgg agacgccctc ttcgctctca tgcgtgagct caataaagct cgcggcacca
    10261 cgttcgtcat tgtcacccat aatgacaagc tctcggctca ggctgatcgg atcctgcaca
    10321 tgcaagacgg ccagatcatc tgacacctcc ttgcgttgtc cgagaagtta tcttcgtctc
    10381 agacttgtct cttgcattca taaagagtcg gtactgcccg gcgacatgat ggcgctcttc
    10441 gctcgatctc gtgtgttgtc atcgcttgga gatcgctgag aaatccttga cgggcgccgg
    10501 ttcatccaat agactcagca acactgcgtc catacggagc gtcgatcaat gcggcctggt
    10561 ctgtcctttg gcgctattat cttcctcgcc ttgttatttg ttcatgggaa ggcgctcggc
    10621 gctgaattcg tgatcgtggg cccgcgtgcc atgggaatgg gcggcgcggg cgtcgcaatc
    10681 accaccgatg ccttggcgac ctactggaat cccgccggcc tggccatgac gcaaacggtc
    10741 gatatccgga ttgtgggcag tgcgcaggcg atcgaccgcc tggggattgc tgacgcggtc
    10801 ggcgaccttg agaactttga taccactgac acttccccgg ccaacgtagc caaggctcag
    10861 gacattgcaa accgcatcaa tcggcctggc gccaacacct cgttcaacgg cgctgcggga
    10921 gtctatgtga aggggcattt cggcgagcac gcgttcggct tcaatatttc caacgtgccc
    10981 accggcggtg gttttgtctc aagtccggtc ggcgtgagtt gcagtcccaa tccgaattgc
    11041 gctgcaccag ggcctatctc atcggtctct gtcagcggtg ccatggcggt tcgggcgctg
    11101 gaggcccggc aagccgcgtt ctcctatgcc tatgcgtttg cggacaaaac gttcgcgatc
    11161 ggcgtcacag ctaaaatcat tcagggagcg gcttacaatg gaactcagaa ccttgtcgga
    11221 ggacaagaga ttaagctctc ggatagttta ggcgaggcaa cgatcacgac gacgtacggc
    11281 atcgacgtcg gcgccatcta tcgaccctct tcctggctgc gctttggcgt ggtggccaag
    11341 gacctcaacc aacccgaatt cgatgcgccg gatggcacga aaatcaagct cggtcctcag
    11401 gtccgcggcg gcatggcact caacccctat tcttcgctga ccttgtcggc cgacgttgac
    11461 gtgacatcga acaagacgtt ggtgccgggg gtcaagagtc aggtcttgag cttgggcgcg
    11521 gagcagacca tcctgacgga atttctctcg ttccgtctcg gcgcgttcaa gaacatgcag
    11581 gatgccgcca ctcccttcac gccgacggca ggacttggtg tacgcatctt tatggtacgc
    11641 ctcgatgtgg gaggcggata tgatttccgc gagaagggag ccttggtgtc cggttccctg
    11701 tcgctcacct tctaacccct atcgtcatcc actaatggta tactgaattc atgtttattc
    11761 gcgcacagca ttgggttcgc gtggcgtcct gctgcctcac gttgatcatc gccggctgcg
    11821 gcggtttgca ggaagtctgg gaaggtcccg gtgccaaaca gtttcgtccg caagccatcg
    11881 ccgttctgcc tccgatggcc agccaatatg acagtgcccg tgaggatata caggatgtgc
    11941 tctcaggagc cctcacgaaa accgccagaa tcgagcgcgt cgtgacgcct gaaagcgtga
    12001 ccgatatttt tcaaggctca aaggaggcct tcgactcact ggtgttctat ttctcgcgcc
    12061 tcgagatgac ggggcaatca gacaaggagt cggccatcaa actggggaag gctttgaacg
    12121 tcgattcatt tctggtcgtt cgggtcaact catgggaata tattcgcaaa gaaggagaca
    12181 acgtgggtcg ggttgggctc agtttgcggc tcgtcgatgc gacgaccggg acaaccgtgt
    12241 ggaaggcgcg ccacgaaaag tcgagtagtt atatgttcta ccgtcctaac ttgaaggatg
    12301 tcgccaacga tctcgccaaa gagatgatcg cgtacatgcc tccccagtac caaaaccgct
    12361 cttcgttgtc acaagggctc gcaagcaagt agatcaatcc cgcacagtcg atcgtgcact
    12421 acggcgccgt accaggaacc atcgccgtct tgagcgcatg cacgaagtta gagacttccg
    12481 acaccatgcc cccgttctga tgtgcggcaa tctgcctgac aatggcgctt ccaacgatga
    12541 cgccgtcggc ataccgagac agacgcgccg catcctgtgg cgtcgccacc ccgaatccca
    12601 ccgcaatcgg agtatccgac agttcccgga tcttttccac gtttgcgccg acgtccgcca
    12661 tgtctgcgag ctttgcgccg gtaattcccg tcaaggaaac gtagtacaca aagcccttgg
    12721 attcccgcgc gacgaacgcc cggcgttgcg gcgtgctggt cggcgcgagt aaaaagatca
    12781 gatgcaaccc ggaagcgcag gccggtcccc gaagcggacc agcctcatca ggaggcatgt
    12841 ccgggacgat caaaccgtcc actccagccg ccgtcgcgtc ctggcaaaat cggtccggtc
    12901 cgtacgcatg gatgctgttg taataggcca tcagcacaag gggaacctgt gatttggttc
    12961 tgatggccgc gaccatcaaa aggatttttc gcaaggacgt gccgcttcgg agcgcccgtt
    13021 ccgctgcctg ttggatgacc ggaccatccg cgatgggatc ggaaaacggg acgcccagct
    13081 cgatgatatc agcgccggct ttttccaatg cgagaaccag ctcttcggtt tcggccaacc
    13141 cgggatcacc ggccatcaag taagcaatca gggccttttc acccttggtc ttcagccgct
    13201 cgaaggtttg gtcgagccgg cttgagctcc tcacagacgg atgtgtgatc gacgctcgag
    13261 catggcggcc agtgcttctc acaaagatat tcccctcatg cgtgcgactt gctgcacgtc
    13321 tttgtcccct cgtccggaga ggttgataag gagaatccga ttccgttgca tcttgggagc
    13381 gatctgaacc gcatgcgcga tggcatgagc gctctcgagt gcagggacga tgccttcctc
    13441 cctggcgagc aaatcgaaag cggccagcgc ctcgtcatcg gtcactgagg tgtaagtggt
    13501 cctgccgatt tcgcgcaaat agctgtgctc cggcccgacg gccgcataat ccagtcccgc
    13561 cgacactgaa tgagtcaagt tgatctggcc atcctcattc tggaggagat acgtcatggt
    13621 tccctgcagc acgccaggct tccctccgga aaaacgggct gcgtgctttc cgctggcaac
    13681 gccgagccct cctgcctcca cgccgatcat ccggaccttc ttgtcgccca agaacgcatg
    13741 aaacaacccg atggcattgc tgccgccacc gacgcacgcc accaggcagt ccggtaatcg
    13801 cccttcggct gccagtattt gcttgcgggc ttccttgccg atgatcgatt gaaaatcccg
    13861 gatcatcatc ggataaggat gtgcgccgag gaccgaacca agaatgtagt gagtcgtgcg
    13921 gatattcgtc gtccagtctc gcatcgcttc gctgatggcg tctttcagcg tccggctgcc
    13981 ggcgtccacg ccggtcacgg tcgacccgag caggcgcatg cggaacacgt tgagcgcctg
    14041 gcgctgcatg tcttccgtcc ccatataaat ttcacattgc aatccgaaca tggcagcgac
    14101 ggtggcagtc gccactccat gctggccagc tccagtctcc gcgataatgc gcggtttctt
    14161 catgcgtttg gccagcaggc cctgtccgat ggcattgttg attttatgcg caccggtatg
    14221 acaaagatcc tcccgcttca aataaatctt ggcacccccc agacgtttgg tcagccgttc
    14281 tgcgaaatag agcggtgtcg gacgcccgac gtattccttg aggtaatagg cgagttgttg
    14341 ttgaaaggcg cgatccttcc gagcttgagc gtattcccgc tccaattcca acaaagcagg
    14401 catgagcgtt tctggcacat agcggccgcc ataggcgccg aagcggccct gttgatcggg
    14461 aatcgtcgcc gggcggggaa gtttcgtatt cacgtcgggc agtataaaca gcctcaggaa
    14521 gaagagacaa cccttacggc gtcgataaag gcgcggactt tttcatggtc ttttttaccc
    14581 ggcaccaatt cgacaccgct gctgacatct acaccatacg gtcgcacggc atggacagcg
    14641 tcggcgacat tgccaggtgt gaggccaccc gcgaggagga tggaggcggt ttttgcggct
    14701 tccgacgcaa gggcccagtc tgccacctgc ccggttcctc cgtacgcgaa ctctgaaaag
    14761 gtatcaatga cgaatccccg tacaccggcc cggccctgat attcagccaa tgccagaaag
    14821 ctgttccgat cctgtaagcg caaggctttc atggccggac gagacagttc ccggcagtaa
    14881 gtcgcgtctt catttccatg cagctgagcg agagccagac cgcaggtgtc catgatcgac
    14941 cggaccgtcg ccagtccttc attgacgaag acgccgacgg cagtgaccag cggaggcagt
    15001 ccggcgatga tccgggcggc gacggcaggc acgatatacc gggggctttt ctcataaaaa
    15061 atgaatccca aggcgtcggc tccagccgcg acggcgacag aggcatcttc cgcattggtg
    15121 atgccgcaga tcttgacttt gagtctcttg gacatgacta agcttgcgcc gacgaacgca
    15181 tcccgcgcag ttcttgtatt ttagtccggg catcgtcggc tctgatcaga gattccccga
    15241 tgagcatggc gtggactccc atttcaagaa gttgttcgac atggccgcgg ttctggatgc
    15301 cgctttcgct gacgatgagc cggtccgacg gaatacgtgg agccagccgt ttggtcaccg
    15361 tgagatccgt ggtaaaggtg gtcaggtccc ggttgttgat cccgatcatt ctggcgttgg
    15421 gaatccattc caggacggta tcaagttcac gctcgtggtg aacttctatc aagacgtcaa
    15481 gtgaaagctc cccggccaag gcatgaaaat cgaccagctg ccgacgctcg agtgccgcga
    15541 cgatcaacag gaccgcatct gcgccatagg cccgcgcctc gtaaaactgg atttcttcaa
    15601 ccatgaattc cttgttcagc accggtaacg gaactgaccg tttgatggct cgcagatagt
    15661 caaggctgcc ttgaaagaaa tccttatcgg tcaacaccga aagagccgag gcacccgctt
    15721 cttcatagcg ttgcgcgata gccacatggt cgaatgcatc tacaaattct gggcgaagca
    15781 ggcccagact gggcgaagct tttttcacct cggcgatcag cgctggactc acggagcttc
    15841 tggtggcatc gagagcgact gaaaatccca atgtgccgga ggtgtcgcgg atttgcgctt
    15901 taagatcggc cagatatcct cggctttgct tatgccgtag ctcggccttc ttgtgctcga
    15961 gaattctggc gaggatcatg cgtgtgcgcc tttgctcgtg aaggacgcca gtcgggccag
    16021 tttgtctgcg gccgcgcccg agtcgatgac gtcgccggcc aactgatagg cctcctgcag
    16081 cgttttcgct tttttccccg ccaccaacgc cggcgcagcg ttcagacaga caacatcgcg
    16141 tttcgcgccc tttttcccct gcagaatttc tcgcgtgatg cgggcatttt cttgtggaga
    16201 tccgccggcc agctcttttg gagagacccg gttcagaccg aattcctggg gagtcaggac
    16261 atagttcgag aggactccgg ctttggcttc agacacgagc gtgcgctcgg taagggttat
    16321 ctcgtcaagc ccgtccatgc cgtgtacgac aaaacaatgt tgcgacccaa ggtgcatgag
    16381 caccttcccc agcagttccg tcagtcgagg ctcgtatacg ccgagcactt gaacggtcgc
    16441 gccggcagga ttggtcaacg ggccaagcag attgagcatc gtacggatgc ccatctcctg
    16501 cctgggccct gcgcaatgtc gcatggcgcc atgatacagc ggcgcaaaca aaaatccgat
    16561 tcccacttca ttgacgcatt cggagacgag gtctggagaa agactgatat tcacgcccag
    16621 ctcggacagc acatcggcac tgcctgactt ggatgatacg gagcggttcc catgtttcgc
    16681 caccgtcagt ccggcccccg ccacgacgaa tgcagtcgtc gtcgagatat tgaaggtatg
    16741 acggccgtcg ccacctgtgc cgcacgtatc gatcacgagc ggagcgccga cggcaatacg
    16801 aactgcacga gcccgcatcg cgcgcaccga ccccgcgatt tcctcaacgg tctccccttt
    16861 gagacgaagt cccatgagat aggccgaaat ctgcgccggc gtggcgatcc cgtccatgat
    16921 ctggttcatg acctcttccg cttctttctc tgtcagcgaa agtcgatcag ccagttttgc
    16981 gatggcgtct ctgatcatgg ccatgcgcgc ggttacagtt tgaggaaatt gcgcagcagg
    17041 tccttccccg ccgccgtgag aatggattca ggatggaatt gcacgccttc cacgccgagg
    17101 gacttatggc gcattcccat gatttctcct tcggccgttt ccgcggagac ctcaaagcag
    17161 gccggaaggc tcgagcgatt gacaatgagc gaatggtacc gcgtcgcgtc gaagggattg
    17221 ggcaattgcc ggaaaatcgt ccggccgtca tgctgtatgg gagacgtctt accgtgcatg
    17281 aggcgcatgg ctctgacgac ttctccgcca aatgcgactg ccagcgactg atgaccaagg
    17341 caaacgccca gaagcggcaa gcgtccggcg aaatgcttca tggtctcgac cgagataccc
    17401 gcctcgttcg gcgtgcacgg tccaggcgag acgacgatgc gcgtgggctt gagggactcg
    17461 atttgttcga tcgaaatctt atcgttgcga tagacccgta cgtcctcccc caactcgccc
    17521 aaatactgaa cgagattgta ggtgaacgag tcgtaattat cgatcatcag cagcatggtg
    17581 atcagtggtc tttgtggtcg tgagggcaga gtaccgtcac ctcatgattg acgtcgctgc
    17641 cttgtcggct cgcgcggatc attccaatcc ctgctcggcc agttctatcg ccttcatcat
    17701 ggcgcgggct ttgttgcatg tctcttcgta ttcatgttca ggattggagt ctgccacaat
    17761 tccggcaccg gcttgtacga aggcctgatg tttcttgatg acgacggttc gaatgttgat
    17821 gcacatgtcc atgttgcctg aaaaactgaa atatccgacg gccccggcat acggccctcg
    17881 tctggtcggt tccagttcat cgataatctc catggcacgg atcttcggtg cgccggaaac
    17941 cgttcccgcc ggaaagcagg cacgcaagac gtcgtagcac gtcttcccgg agtccatctg
    18001 tcccgtcaca ttggagacga tgtgcatgac gtgagaatac cgttcgacgt gcatgagcga
    18061 gtcgacgatc acggagccgg gacgggcgac tcgccccacg tcgttgcgcc ccaagtcgac
    18121 caacatgacg tgctcggccc gttccttctc gtccgccaac agacggcgct caaattgctg
    18181 gtcctcttct gtgctttggc ctcgccgacg ggtgccggcg atgggtcgga cggagatgac
    18241 cccgtcctca cagcgcacga gtgtttcggg ggacgaaccg accagttcaa gccccgctac
    18301 acgcagatag tacatatagg gagacggatt gatcacacga agggcccggt acagttgcag
    18361 cggagaggcc tgcacattgg tttcccatcg ttgggacaag acggcctgga ccacgtcacc
    18421 ggatcgaatg tattccttcg tccgtgtcac catcttctca aagtcggctc tgttcatgtt
    18481 cgatgtaaag ctgatgggaa gacggcgccg atgagggcgt gtcctggcga gcggacgctt
    18541 gagacgtgcg atcatgcgtt cgatgcgatc agtcgccgcg cggtaggcct ccctgacatc
    18601 ccgttcccgc gtggacgcga catgcgcgtt ggccacgact ttgatcttgt gagagacatt
    18661 gtcgaaaatc aacaaggtgt cggtgaggag aaaggcaaaa tccggcagat tcgtgtggtc
    18721 cttttgcctg acgggcaact cctcaaacgt tctgaccatg tcatagctca aatatccgac
    18781 ggcgcctccc acgaaacgcg gaaggtccgg cacagtgacc ggatggtatg cagccatgac
    18841 ctcccgcaat cgctcgagtg gatttcccct gctgaggatg cgttttttcc gcttgcccct
    18901 gacttgtatg agatcccccc ggtcttcgta aatgactacg ggcgagccgc ttcctaaaaa
    18961 ggaatagcgc gcccattttt caccgccttc gatgctttcc agcagatagg cactgggccc
    19021 atgatcgatt tttgcgaacg cggagacggg ggtctcgtaa tcggctagaa tttcgcgata
    19081 cagcgggatg aggttaccct gtcccgcgta atgccggaat tgttcgaggc tgagtgaata
    19141 cgctggtaca gacacggccg ctgtgcctat tgttcctggc tgacgactaa tttctgtccg
    19201 acttcaatga tatcgtccgg cagcttgttc cacttacgca gtgtgtctac tttcacacca
    19261 taacgccggc tgattcggta taacgtctcg ccgggcttga cgacatggac cgtggaagcg
    19321 cctttccctt cgacttgcgc gtcggacgtc gagaatgcca cgtccgactg agcgttgggc
    19381 gccgttccca ataactccgt gatggcgggg ctggattcga cgggaggcgc cttgacggca
    19441 ggaggtgctg agcgcttcat gtccgccttc gctttgcgtt cgaggagcga ggcttccttg
    19501 atcgcctcgg cctcctcctg aacacgcgac atctgctcgc gtacagcttg aacttgagcg
    19561 gttaactctc gattgcgcgt ctcgtattcc gccaactcac gcttggtccg tttcacctca
    19621 ttgtcgagct cgccggttcg cttttcttcc tgggcaagca gccgctggaa gttcaaactt
    19681 cgcgcttttt ctgcttcgta cttttcagac agcacacacc cgctcagaca cagccctcca
    19741 cagatgagag cgacgacccg taccgccggt cgaagcgatg gtctgacgct cgttgcttcc
    19801 aacatggcta cctcctcatc gcacgtgaac tctctcgtgc gctccgatca cacgcggttc
    19861 tgtggtataa aaccccttca gcaggattgg atagaataat ggctaacgca gcggaagtca
    19921 aagcgaataa ggtcacttcg tcgtttgacc atgccggcgc ggctatgata cggtcgtccc
    19981 gcttcggggg tcatccttcg cccgatcgtc tgcgcggttt ctccaaactc agtctccatg
    20041 ggcactactg atatccaagc tcgagtggct gccttacggg aggagattcg gcggcatgat
    20101 tatctgtact atgttcaggc caagccggaa atctccgatt ctgcctacga tcgcttgttt
    20161 cgagagttaa cagaactcga gcaagcccat cctgagctgg ttacggccga ctccccgacc
    20221 cagcgcgtgg gagctcctcc cctcgattcc ctgagcaagg tccgccacga acaggcgatg
    20281 ttaagcctcg attctctggt cgaacagagc gatgtcctgg catttgacca gcggatgaag
    20341 cgggaattgg gtgtgtcctc ggtcgggtat acggcagagc caaagtttga tgggctttct
    20401 gtggaattgc tctacgaacg aggcttcttt accagaggag cgaccagagg cgacggggtg
    20461 acgggagaag atgtcacggt caacttgaaa acgatccggg ccttaccgct gcaactgatg
    20521 ccgggaacgg gctatcccga tcgtctcgtg gtgcgtggcg aagtctatat gaagcttgat
    20581 gagtttcagg ccatgaatcg aaaactgacc gaacgcggcg aggacggttt tgccaatcca
    20641 agaaatgcgg cctcaggcgc attgcgacag ctggattctg ctatcacggc tgcccggccc
    20701 cttgccctga cttgttatga aatcatggca tggtccggca agggcttgcc gacacactgg
    20761 gatgagcttg aggccctggc gcgatggggg ttccctatcc cactgcatcg cctcaaatgc
    20821 gactcgattc aagaggtcat cgcctatcat cgagagactg aacacctgcg cgacagtctg
    20881 ccctacgaga tcgacggcgt ggtgatcaaa gttgatcggc gcgattggca ggaacgactg
    20941 gggtacaaat cgaggagccc gcgctatgca atcgcctaca aattcacacc tcgcaaggaa
    21001 atcacgaaag tgaaggatat cgtcgtgtcc gttggacgga ccggcacgtt gacgccgctt
    21061 gcgttgctga cgcccgtgga agtcggaggc gtaacgatca gccgagcgac tcttcacaac
    21121 gcggatgagg tggccagaaa ggacatacgt gtaggcgaca cggttcgagt cgagcgggcg
    21181 ggtgacgtca ttccagcgat cgccgaacgg gtacccgtcc ccgacgagcg ccgtggagaa
    21241 ccctttcgga tgccggatca ctgtccagtg tgccagtcca gcgtggcgag ggaaggagcg
    21301 tattattatt gcaccggaca atcggtctgt atggctcaac tcaaaggagc cattgaacac
    21361 tttgcgtcga aaagcgcttt gaacattgca gggcttggac ggaaaacggt ggcacagttg
    21421 gtcgatgcag gactcgttca ctcgcttgca gatctctaca ggctgaccag agaacaattg
    21481 ctcgcgctgg agggatttgg cgagcgatcg gctggcctgt tgcttgaggc catcgaacga
    21541 agcaaaagag tctcgctcga ccgtttctta atgggtctcg gcatccgaca ggtcggacag
    21601 cacattgcca aagttctcgc ccgggagttc ggttcactca ctcgtatcat cgactcagac
    21661 gacgcgagat ttcagcaggt gtcggcaatc ggcccagaga tttcttccag cttggtctca
    21721 tatttttcag aagcgcacaa ccgccgcgtg attcaagact tgctcgatag cggattcgac
    21781 atcctcgccg aatcggttgc ggcatccgca cggcagacat tagccgggaa aacctttgtc
    21841 ttcaccggtg gcctcaagga cttgaccagg gaacaagccg gcaagttagt tgaggagcga
    21901 ggaggacgaa tcgcctccac tgtgagcaag aaaaccgcct atgtcgtggc tggagcagat
    21961 gcggggtcga agctcgaaca ggcggcaaaa atgggtatta ccgttctcag cgaagaggag
    22021 tttcggcagc tcgtcgaatg accaagccgt gtcagctaac gagattcaaa ttgggagctt
    22081 ctacggcaac cggggttttt tcttcgcggt agatttgcac gcttcggatt gaacgtggct
    22141 ccgcttcgtg gatgacgata cggcagttgc cgattctcag ctcttcgccg accttgggga
    22201 tgcggcccag ctcctgttga atcaacccgc tgattgttac cgcttcgtcg ccgagatcga
    22261 ctttcaggaa atcgtttatt ttgcggactt cagtgcggcc atggacaagg atatgatttt
    22321 tgccgaggcg cttgatcaat tcctcggtga tgtccgtttc gtccatgatt tcgccgacga
    22381 cttcctcgag caaatcctcc agggtcacaa tgcccatgac gccgccgaat tcgttgacca
    22441 ccacggccat gtgccgcttt tcctgctgga attgtttcat gagatcgtcc gctgtcttcc
    22501 cggccggcac aaagatcggc ggataggcaa tctccttcag cttggcgtcc gtttgattct
    22561 tggccagctc cgtcaacgcc ctgcttttgt aaagaatgcc ggtgatgttg tcgagcgttc
    22621 cgtcgtagac gggaatgcgg gagtacttcg agttatacag gagctcttga gcttccttca
    22681 gacgaaggtt cccgtccagg gaaaacacat agagtcgagg cgtcatcgca tcttcagcag
    22741 taatgtcctt caattgaaag acatttttga tcatcttaac cttttcgacc tcgatggcgc
    22801 ctgttttgcc gccctcgtcg agcataatct tcagctcttc ttccgtcacg agcggcaggg
    22861 ttaatccctt tccgcctgtc agtcgatgaa taaacggcac catgagtgcc aagatcggtt
    22921 tgagaaccaa ctgcactccg tagaccggat aggccatgtt caatgtcacg ggcaccgcga
    22981 acttggctgc caaggttttc gggacgacgt cgaccgacac cagaagcacg aacgtcagaa
    23041 gcccgaccac gacggcaatc gtatcatcaa agaacgtccg gtcggcccca taggtattta
    23101 aggtgaggaa tgtcgcatac atcggaatgg cggttcccac gagtcgatcg ccgaccagga
    23161 tcgtagccag tagtttctgg ggatcactac ggaggtggag ggccatcttg gcccggctgt
    23221 tctggttttg agccaaggca cgcagtctgg tctcattgac cgcaaaaaac ccgatttcag
    23281 cagttgaaat tacagcagaa agaacaataa ggcccagcag aatggcgata tccatagagc
    23341 ttccaggttt agggacgggt cacttgcaca gtggcctggc aaatgggggc cagaaagccg
    23401 ctgccggctc tcgggcaggc gagacacatg gcggagatac cgagatcgat gcgaaagact
    23461 ttggggggga ctaagagttt catgaggagt acatctatca taggcaaggt tagggggcaa
    23521 gcacttcaac aaaagtgatc ataaaatcaa aggcttgtgg atcacgctta attgtcgtcc
    23581 cgaaacacga aggactgagt ggctaagtca gcctccaatc tcctcgctgc ggactcggct
    23641 tgatgctcgg tcttaaactc gcctacatga acgcgatagc ggcggccctc gggaaggtcg
    23701 accggttgga ttcggcctcc tggatataga cgtcgagccc gttccaatag gttcaaggca
    23761 ttttgctgct caacgaagga gccgatttgc acccgaagga atcccatatc ggcggttctc
    23821 ccttgaaatc cgatgacgcg cagttcgatc cggtctgttc cactgccgat cattccgaga
    23881 gcctgggcgc cggccattga aaggtccagc acgcggcctt tggcaaaggg gccgcgatcg
    23941 ttgatacgga cagtgacttc ccggccagta gtggccgagc ggatcgtagc aatggatccg
    24001 agcggcagtg tcctgtgagc cgccgtgagc ttatgcatat cgtatcgctc accgttggca
    24061 gtccgatttc cgtggaatcc cggtccgtac caggaagcca ctcctccctc gacataaccg
    24121 atggggtagc cgggaaaata ggtcggctta ggcggactgc tacatccgat gactgtggct
    24181 gtgacaacaa gagcgctgac gaaggccggt gagaaccgga agccggagag caaaccacga
    24241 ctgatggtca gtaagcagac acgcccatca gaggatccag gagggcagat cggcatcagc
    24301 caaggatgag agacagtggg gcaagcatgg ctgctaaaac tcgtcgagag actgatcgcg
    24361 caagattgca agcgccttct cagtattggc gaccgtgagg accacgatcg cacgcttacc
    24421 ttccctggag ggcgtgcaat acccgcactt gatgttaatg cgagcgcgcg tcaataagtc
    24481 ggccacctcg ttcagcgcgc cgggcttgtt ttcgaggctc agaatgaggg cagtttcctc
    24541 agtgaacttg attttagctc ctctcaaggc ggtacgcgcc ccctcgacat cggcgaccaa
    24601 aagcctgagc ttaccggttc ctgtgacttc cggagctgaa aacgccttaa tgttcacacc
    24661 ggctgcggct aacacagagg cgacctcggc catgactccc ggacggctct gtccgctaat
    24721 gacgaattgt gtggtggtag gcatatacct gtcctaattg aatacccccg cttaagactc
    24781 ctatcacttc acacgggtaa agcgcaacaa taaccctcca acgggtgcag tcctcagcgg
    24841 ctacaaaatg tgacagactc cgttgataac cgtgtattca tagatgcagc ttatgacaag
    24901 agaagcatcc tgcgtcagct tctggacgga atctttcaag cctcgcggca ccaagaggta
    24961 aaaagggatt cctctcttca tcgtccgcat ccacttgtcg gcgatctgag ggtccgacaa
    25021 tgacgcttcg gtttcaactt ccgccatcca ctccatcgtg tcgccaccgg agctcacata
    25081 ccaccccaca atatcgcaac tctgttctgg actcgcccag gaatggtgtt cgacactccc
    25141 ttttatcgtg actttgcata cgaaggcctt ggcccaccgt tgagccacca ggccgacgac
    25201 ctgatcatga accgtttcaa ggccacgttc gtaaagtgaa gtagtcatgt cccacggcct
    25261 ttatatgtca aaacctctcc ttccttccta gagagaggcg agtggttaag agcatccgct
    25321 ggttgcaccg caggtctcgc acttcaaaca cgtcccattg cgcaccatgg tgaattgctt
    25381 gcattccgaa cagggatcgc cctcatagcc tttttgccgc gcaatctgta cggccgtcat
    25441 cgtcagcgtt tcgcgcttga gctccacctt gtgcgtgacg ctgccattgc cgtttccatg
    25501 gccgttgccg ccatgtctcg gctggatccc tcgacgggac ggaaacatct ctgtgttaat
    25561 ggatgaggac gccagcatct gcgggtcggc ctcttcatca gaacattcgg gatcctggtc
    25621 gtcctttttc acggaatcca tccgcaagtc ttcttccttg acctgtgcaa gatcgtatcg
    25681 atccaaatat gtcacggcca gctcacggaa aatgtaatca atgatcgacg tcgacatctt
    25741 gatccgatcg ttgagcttca cgggtccgtt gggctcgaag cgcgtgaaca cgaaggcttc
    25801 gacgaactct tccagaggaa cgccgtgctg caggcccaac gaaatggcga tcgcgaagca
    25861 gttcatcaag ctgcggaacg cggccccttc cttgtgcatg tcgagaaaaa tttcgcccac
    25921 tgtaccgtct tcatattcac cggttcggag atacagtttg tgcccgccga tgacggcctt
    25981 ctgcgtatag ccattgcgtc gtgccggcag cggccggcgc ttggcaagat accgcaccag
    26041 gacccgttcg gtgattttct cagcgatggt gagcacctct gtcgaagtct cgaggctttt
    26101 cccgccatca ctggatgccg acaacggctg actcagcttg gaaccgtcgc ggtacaatgc
    26161 cactgccttg accatgctct tccaggcgaa gaggtacgcg gatttcacct cttccagcgt
    26221 cgcgtccgcc ggcatattga tcgttttgct gatggcgccg ctgatgaacg gctgcgcggc
    26281 agccatcatg cggatgtggg catcaacagc gatgtaacgc tggccgattc tgccacaacg
    26341 attggcgcag tcgaatacgg gaagatggtc tgatttcaga tgtggagccc cttcgaccgt
    26401 catcgccccg cagcaatagt cattggcggc cgcgatctcc tcctgggtga atccgagcgc
    26461 cttcagcatg ttgaaattgg cttcgatcag ttggacgtcc gtcagtccga gttttgcccg
    26521 gcagaattcc tcaccgagca cgtacttgtt gaaggcgaac tggatttcga acgcctgtga
    26581 caggccggct tccatgcggg cgagcgccgc gtcatcgaac cctttctgtt tcagcgtctc
    26641 atgattgatg aacggcgcac cttgcaaggt ttgccggccg acacaatagt taacgatgtc
    26701 ttggacctgc gcctccgtat accccaacgt gctgagcgcc ggtggcaagc tttgattgat
    26761 gatcttgaaa tagcctccgc cagccagctt cttgaatttc accaacgcaa agtccggctc
    26821 aatgccggtc gtatcacagt ccatgacaag accgatcgtt cccgtcggag caatgactgt
    26881 tacttgcgcg ttgcggtacc cgtaggcggt accgagctca agagcacgat cccaggcccg
    26941 tcttgcggca atgagcagat cgggcggaca atgttctgcg tgtatgctcg ttggtttgat
    27001 tgtcaagcct tcgtattctt cctccggcgc actataggcg gcacgacgat ggttgcgaat
    27061 cacgcgcagc atcgcctcgc ggttttttgc gtagccggga aacggccaga gttcggccgc
    27121 catttctgct gaagtactat aggcttcacc ggtcataatg gaagtgagcg cgccgcagat
    27181 ggccaaggct ttatcggaat cgtacggaat accctgccgc atgagaatcg tgccgagatt
    27241 ggcatagcca agaccgagcg tccggaactg atagcttttc tccgcaatgg aacggctggg
    27301 gaaggatgcc atcagcacac tgatttccaa cacgatggtc cagatccgta cggcgtggcg
    27361 aaagtgctcc agttcgaatt cgccttccga cgtgaaaaac tgtgccagat tcagcgacgc
    27421 caggttgcag gcggtgtcgt cgaggaacat gtattcggaa caggggttgg aggcgttgat
    27481 gcggccgtct tctggacagg tatgccactc gttgatggtt gtgtcgtatt gcgtgcccgg
    27541 atcggcgcag atccatgcgg cccacgcaac gcgatcccac aggtcccgag ctttcacggt
    27601 tttcgcgact ttcccgtcga tccgccgctt gagttgccaa tcgccgtccg cttccagcgc
    27661 ggcaaagaat tcattgggaa ttcgtacgct gttgttcgaa ttttggccgg aaacagtttg
    27721 ataggccttc ccgtcccagt tggtatcgta ttcatggaac acgaaatggg tgaatccctg
    27781 ttgagcataa gagaacattc gttgaatgta cgcttcagga acgttgtcac ggcgagccgc
    27841 cgccagcgct tccctgagga tggagttctg cttcgggtca acggagacac gcggggcacc
    27901 ttccgcgtca acgatatggc aggctttcag cactgcgttc aagcgttggg cgcagatctt
    27961 tgatccggtc accattgcgg cgaccttttg ctcttcgacg actttccaat caatgaattc
    28021 ttctatgtcg ggatggtcca agtcgaggca gaccattttg gcggcgcgcc gtgtggtgcc
    28081 tcctgattta atcgcccctg ccgcacggtc gccgattcgc agaaatgaca tcagaccaga
    28141 ggagcgccca ccgcctgaca gggcttcacc gtctccgcgc agtttcgaga aatttgttcc
    28201 cgtccctgat ccatacttga agagccttgc ctcgcggacc caaaggtcca tgatgccgtt
    28261 ctcgttgacc aagtcatcct cgatagactg tataaaacag gcgtgcggct gggggtgctc
    28321 gaaggcattt gtcgccttga cgacttctcg tgtcttcgga tcgacgtaaa agtgaccctg
    28381 ggcggggccg gacaatccat aggcgtaatg caagcctgta ttgaaccact gcggcgaatt
    28441 aggcgcggcc atttgatgtg ccagcatgaa gcacagctcg tcatggaacg cggcactatc
    28501 ctctattgtc ttgaagtagc cgtaggtttt tccccaatgc gtccaacaac cggccatgcg
    28561 ttcaaatacc tgtttggcat cccgctctcc gcccagcgct ggtttgccgt ctggtcccaa
    28621 gatcgggttg ccggcgtcgt ctcgctgcgg cacacctgct tttcgaaagt atttttgcgc
    28681 caagatatca atcgcaagtt gcgaccagga ttcggggatg tcgatgttgt caagcttgaa
    28741 gacggtcgat ccatcaggat tgcgtatctc cgatgagcgt ttcacgaact tcaacccctc
    28801 atagggactc tgtccggaac gagtaaatcg ccgttctatt ctcacgttgc ctcctccttg
    28861 gacgaggtaa tttgcaggct gatgttggct tggctaccct acggaagtac tagacaaaag
    28921 gcctcaaccc tcatgctttt tttccctact tgggctcgtt cggcataagt cctttagccc
    28981 catcgagatc gcggagtgac tacatatagc tattggagtg aattgtcaac actaaatatg
    29041 gtggtttatc tgtggaaacg gaggaaaggc tgtggataag agaaaggtct ttcccagggc
    29101 ccatccgcac aaaaagttcc aagcttatcc acagctgtgg atagtgaagg gggatcattt
    29161 ggctgcgtga agcgggaata aagatgaagg actaaaaggc caggcgaccc agacgaacga
    29221 tcgcttcgtc tattccctgc aatggaataa aacgtccggc aacgccgagc acaacaatgc
    29281 gggaattgaa cacgctgctg tcataaatgc tcacaagtcc ccaattgcgc cggaccgtgt
    29341 gcggacgtac aacgctgtcg accaactcat ggctgtgcga gcgaaagatt gtgcgcacca
    29401 tcgcccgttc cggcgagagt tgtgcttcgt cgctgcgctc aatggccttg gtaagttcac
    29461 gctcgacgaa ggtcaaatat tcgtcgagcg tcggattgga aagggcgagg ccaacgcata
    29521 ggctcagcgc cgccaccagc atcccgagcc ggaacagagt cacgacgatt cccctttgac
    29581 aaacgtgagg tgcatcatta gcctaccggc aagttcgtac tgccaattgg agtctaacat
    29641 cgcgaacagg gcggagggca tactatgttc gtatggagca agaagctgat cgcagcgctg
    29701 ctggcgttat tcttggtgct gttgggagta tttttgattc atggcgcgcc ggacagcgcc
    29761 agtgggctaa aaattcacac tacccggtgg atggcgctca attacatcgg actcatggtt
    29821 tgggtctttg tttggatgat tgatcagacg cggatgcggg gtaaaaacgt atggatctgg
    29881 ctcatgccgt ttgctctggc acccttgccg accttgatgt tgttcgtact ctttcttcag
    29941 cgtcgtctta agccggtttc atgactggag agtccggcga agtctcgtcg gataggtcct
    30001 gaggcagctg gcgccggtag agccagacga gccaacaacc gggcagcgcc aacacaaacg
    30061 aaagaaaaaa gaaccagtcc catcccaata tgcccacggc ttcagccgaa ggacgtccgg
    30121 caaagacccg tccgagggct tcgagagaag acaataacgc aaattgggtg gcagtgtagc
    30181 gatggtcgca gagcgacatc accaaggcca caaaggccgc ggttcccatc cctccggtta
    30241 cattttctat gaccactgat gccgcaagag cttcgtaact ttttccgatc caggccaaga
    30301 acataaagga aaggttcgag aaggcctgga gcaggccgaa aaacagtagc gaccgaatca
    30361 agccagacct ggccatgatc aggccaccgg ccagtgctcc gattaaagtc gctgcgatgc
    30421 ccagaccttt gacatacccg acttcgccgg cggtaaagcc aagtccgccg atgaaaaagg
    30481 ccgtctgcag cgagccagcg atggcatctc cgagtttata caggataatc aagcacagca
    30541 gtccatatat accagggcga gcaaaaagtt cccttatggg aaagcccact gcctccttga
    30601 gcgttctcgg ggcggcaccc cctcccggag ggttcggact aagaagtatg gtgacgatgc
    30661 ctgcccccat caaggcagcc agcaccaggt aggttccttt ccaaccaatc tgctcagcaa
    30721 ggacgagggc gcccgcgctg gccactaaca tagccagacg atagccgttc acccacaagg
    30781 cagcgccgaa cccccgctcc gctggtagga gtaggtcggt gcgataagca tcaaaaacga
    30841 tgtccaacga agccgagcag aaggcaacgc caacagcgat aagtcccagc caatgggggc
    30901 tctcactgct gccgatcaat cccattaagg ccaagcccaa cgccacagcg atttgcgtga
    30961 ccaacatcca gcctcgccgt cgccctaacc aaggcagcga gatccggtca atgaatggag
    31021 cccagagaaa tttcaagctg tacggcagac ctattagcgt gaagattccg atcgttttca
    31081 gatcgagtcc ggctaccgtc aaccaggctt gaagggttcc cgacgtcagg gccaatggca
    31141 gaccagaggc aaagcccaag ggcagcatca gcgcaacgcg cttattggca aggatgtgtg
    31201 cgaatgaatc cgcagccatg tgattctcgt gactcgtgtg ggtctagagt aacactggac
    31261 cgctgactcg ttgaggagaa tttggaattc tgtaggagca agcgattcgt tcaaaaccgg
    31321 acagaaagtg agaactgctc agcaaggctg tccgttgaag gtgcagggtt tgtagcggga
    31381 cacgtcgaat tcgatgtttt cctggtagat gcgttcattg ccgttgacgc cgtcttgctc
    31441 gggatcgttc cacatattgt gattccacca ataaaagagc gggtggattt ccgtttgata
    31501 gacggggttg ccgaaattac tgcgggcgta ttcctggacc atccgcccgg tcacataatc
    31561 gatctgaccg tcgcccttta gcgaatacag catgagaaac aaacgagctt catggtcgta
    31621 caactcgtcg atgcgagtgc tgagatcggg ctcaatcggc aagacgtcct tagaccaggc
    31681 atgctcctga acaaggatgc tcaaggatat tacgcaacaa gcggtgaatt ggcggatcat
    31741 caccggtgtc ctcatgcggt aagtcgttta cggcatgcta gcacggtgct tcgagccctt
    31801 caaccatggc tgtccccccg ttgccgcata cttgcccgcc gcgcaggcac gtctctataa
    31861 tgatcgcccc atgatcaagc gcgcgtcatt gcatagtctc ggctgccgtt taaaccaagc
    31921 cgaaacatcg gttctggcgt ctcaattgcg gcgccaggga tatgctcttg tcgactttgg
    31981 agagccgacc gatcttctcg tcgtcaatac atgctcggtg actgaggaag cagaaagaaa
    32041 ctgccgctat gcgatacgga aaaccttgaa acattcgccg gatgcattcg tcgcggtgac
    32101 cggatgttat gcgcaaaccg ggttgcaaag cctgcggagc attcccggca tcgatctcat
    32161 cgtaggcaat cagttcaaat cggatttgcc ctcatttctg ccgccgccgc aaattctgcg
    32221 caaacaacca gcgccagaag tacttcacac gaaaagcatc gatcgacacg acttcgttct
    32281 tccagagttc ggagagtctc attcaacaag agccttggtc aaaatccagg atggttgcaa
    32341 tgtaatgtgc agtttctgcc tcattccgtt tgcccgcggc cgcgagcgaa gccgcagggt
    32401 cgatgacatc ctttcggagg ccaccgcttt ggcggccgct ggccatcacg agctcgttct
    32461 cacaggtgtc aacatcggac aattttctca gggcggggtg accctcgtcg gtttaattga
    32521 tcggctggag ggaatacagg gcatcgaacg catcaggctt tcgtctatcg agccgactac
    32581 ggttacggac gatctgttgg aacgcatggg ggcgtctcgg aagctgtgtc cctatctcca
    32641 cgtcccgcta caaagcggag acgacgcgat tctcaatgcc atgaaccgtc cctatgacgt
    32701 caagacattt atctccctcg tcgagcgggc gacggcaagg gttccggatc tgacatgggg
    32761 aaccgacgtg atggtgggat ttcccggaga atctgaccgt gcttttgagc gcacggttga
    32821 tgtgtgccgt cagctgccgt tctcgtattt ccacgtgttc acctactcca gacggccggg
    32881 caccgcggcg gcaagaatgg ctggcacgat tcctgtcaac gtcgctcgat tgcgggctaa
    32941 gacgttggct gatctctcgc gagacaagcg gtttgccttc gccgaacgat ggattggatc
    33001 gtcagtggcg gtgctgtttg aaagcggcac caccgagggg ttccgccttg ggaccacgag
    33061 gcacttctta cgagtggctg tgtcgtcgga aacccaattg gcgaatcaga tccatcctgt
    33121 catcatcaca ggggcatcag accgatgggc cgtgggacac attcacaact gaaagggcac
    33181 ccacattgaa tcgtaaaccc ggcatgcgtc aagtccacat tgagaccttc ggctgtcaaa
    33241 tgaacgagta tgacaccgag ttggtccgtg ccctgctcaa gaaggcgggc tatgggttca
    33301 ctgaggatcg agaatgcgca gacgtgattc tcatgaacac ctgcgcgatt cgcgaaaacg
    33361 cgcatacgaa ggtgtacacg catttatcag acctcagggc gctgaagaag agcagaccct
    33421 tggtggtggg ggttctgggt tgcatggcgc aaaatctcaa agcggaactc accaaacaag
    33481 agccaatggt cgacctcctg gcgggaccgg actcctaccg tcagttgccg atgctgatcg
    33541 atcacgccat gcaagcgcaa gaagtaggtg tgcatatgca gcacctggcg gtggatttat
    33601 cggaatacga aacctatgag ggcatcgtcc cggctcgtct gtccggggtc aacgcctgga
    33661 ttgctgtgat gcgcggttgc gacaacttct gcagtttctg tgtcgtaccc tatactcgtg
    33721 gcagagaacg atcgcgcgag ccggatggga tacttgagga agcaagacgt atcgcagcgg
    33781 aaggtttcaa acagatcacc ttgctgggac aaaacgtcaa ctcttaccgg tcaggggaat
    33841 gggatttcgc gcggttgctg gccgccgtgg cggacgtgcc cggaatcgaa cgcgtaaggt
    33901 ttacatctcc ccatcccaag gatttcccgc cggctttgct ggaggctgtg gccttccatc
    33961 caaaaatctg caagcacatt cacctgccgg tccagtcagg aaacgatcgc atcctgaatt
    34021 tgatgaaccg ttcctatacg aggagggaat atcttacgct cgtcgaacag atccgatcga
    34081 taaatccgga cattgtcctg acgacggata ttattgccgg attttgcacg gaaacggagg
    34141 gggaatttgc agatacctat cgcttgctcg aagaagtccg ttatcattcc gcgttcatct
    34201 ttgtgtactc ggagcgcgcg aataccatcg cggctagaaa gttcccggat gacgtggccc
    34261 ctgaagtcaa atcccaacgc gtgacggcac tcgtggagat gcagcgccgg atctccagcg
    34321 aacgcaaccg cgaatacgtc aacaggacgc taccggtgct tgtcgaaggc gacgccaaaa
    34381 agtcccggct ccaagccatg ggaaaaacgg acggacacat tacggtgata tggaacaagt
    34441 gtgatctgcc ttctcgcccc ggccaagtgg tttcatgctc tatctatgac gcgtcggctt
    34501 cgacgcttta cgccaaagct ccgacttttt cggatctgca ccggccggaa aacgaacact
    34561 cctgcaccaa tagtgaccag ggcgtagctt aatgcaggcg gtgcctattc ttctcgagcg
    34621 ccgccggatc tcgcctcaga agcgtgaaca cctcggcagt tccagcatag attctctacc
    34681 gcttctgtca tttaccgcca attagatcaa agtcttatac agcgtccgga tgcgcgccgg
    34741 atcgacagac gcttctccac ttctcagtca ggatcagctt cacttctaac cattcaggta
    34801 aagattatca gggcatagtc tttgctttga agatgacaga ctttcgtaat tcccatccat
    34861 ttttgaacgg agcacgggat gaattcctct tcaaatcaga cggagaacgc tcaggagcgt
    34921 gagacaccca ccctcagcct cgaggccatc attgcaatgg ggttgtttgg atggctggcg
    34981 ctcagtatga ctctgatggg tttggggatt tggtagcgct tttttccgtt gtcggtctgc
    35041 cgattcgccg tcttgccccg cctgcttttg acaaaggaga acagggatga ccatcttttg
    35101 ttccgttacc cgggccatgg ccccgatgct ttgctgctgc ctcgtcctag cctcgcttac
    35161 gactgggtgc tcttccacgt caaagatcgc gcaaaatggg agaggcagtg tctttcttga
    35221 agaagtaatg gattggactt ttgaagcaag ccatcctgcc gtaatagacc agatcacaat
    35281 attgaagatc atgaaaggcg tgcacagtag caacgctcaa caggggtcca tgagaatgtc
    35341 cgccggtggc agcagcaagc cgatgagagt attcagcgat gaggatgcag aatttctagc
    35401 tcccctgctc gcacaaggct tatcgaaagc gaaacccgag cagttggtcg ggttccgcgt
    35461 gtcctcatcg gcagggtcag gctctgagcc cacggcgggc agcatctacg tacggcagga
    35521 tgcgattcat ttcacggttt cgagaggtgc cggaccgacc atttttctcc ccgaatcggt
    35581 cgcccgtagc gagcaggctc cggcctacgc cacgggtggc aagacgggag cggtggctca
    35641 tgtcatcgat tatcacatgc tggccagggc acccatgcca gctctgcccg cgatgaaaac
    35701 atccgctgcg cagaccgcgt cttctccggt caccgccccg ggagttatga cgagtgcgac
    35761 agccgtctcg gcctatgaag gcatcgagaa cggcagttcc gtggaaactc aaacacagct
    35821 gcgccgggca aaggacatgc tgaccaagaa agacacagag atcaatatgc ttcggaagga
    35881 atcgacgtgg atgaagcgcg agctacgtga gcgtgatgat gagatcaagg cgctgaaggc
    35941 caccgtcaag aagacgccca agaagaaagc ggaggcgtat ccgacccatt agcgggtcat
    36001 cccacatcaa gcaactccgg ctgtagcagt acgacttcgc cggacagcgg ttgacgaaaa
    36061 ttcatccggc agtagcaggc gtatgtgaca tacgtatcct gcaagcagta gcgtgccaac
    36121 tccaccccct ccccgcgtcc ccacatctct gcgacctggg ccccgctgcc tgatttggtc
    36181 tccacgttca atgcccgcgc caatacgtcc agcttgaccc agcaccgcat gtcccaatta
    36241 ctccagatcg ccatggtgtc gtagacaggt tcggatctga acttggccag attgatctcc
    36301 atgctgggtt tgacctgatg gatgatcgat cgctttctga taaacggaag gtcaaatccc
    36361 agtccattgt gcgtaatgaa caacgacggc ttatcttggg ccaaacggct ccagaactga
    36421 cggagaagtt ctcgctcgtt gttcccgtac caggccaccg ccccacgcgg ttccatttga
    36481 tcggaaaatt cgagaagccc gatgcagaca atcgaactgt aggtgccgtc gaaggctgat
    36541 ttggcatact gctcgttctc ggcctgccgc tgcgcctcgg cttccccggc ggcaaacagg
    36601 tcatattccg gttcttcgcc aatgggcgaa tagcatttcc cagccaagcg cgcccagtct
    36661 tcgcgcgtag actggatcgt ctcgatgtca aggacgactt tcatgtgttc tgacccatcg
    36721 aatcccgctg caacgcctca ataaccggcg cgtcggccgg cgggaattca tactgtccca
    36781 tctccgaggg atggacccaa cggatttccg cgcaatccaa cggagtcgcc cggccctcac
    36841 cgatcgtgca ccggaagaag tgcagttcga ccgttttttc aggatattca tgccggatga
    36901 ttcgaaacgg aattggtcca tcgatccgaa tgctgagttc ctcgaataat tcgcgctgca
    36961 aacactcctc cagcgtctcg cctggttccc ttttcccacc tggaaattcc cataggccgg
    37021 ccagatggac gcctgtccgg cgcttggcga tcaggtagcg cccttcatga gcaattaagc
    37081 ctgcagcaac ttcgaccacg tttgtcatca ggccttgcct cgtggcgtga acggaaaggt
    37141 cctgcaaatc ggcttcatcg tgcatccctg gcaggacggc gtccgggccg tgcaacagag
    37201 cgcaccgaaa tccatgatcg cctggttgaa atcatatccc cgccctggcg gaatcaacgc
    37261 ttcggccagt ttccacaatt tgaccttttg cctcagggga ttgcccctgc cgatgaagac
    37321 ccggtgaaga acccgcatga catttgtatc aagaatcgga gcatcttcat tgaaggcaaa
    37381 cgaccggatg gctccggccg tataccggcc gatccctttg aaagacaaca gttgctcgtg
    37441 atcattgggc aatataccgc cgtaccgctc gaccgtttca caggcaatgc tatgaaggcg
    37501 ttctggccga atgttgtagc ccagtggata ccaaatctgt ttgacctgat tgaccggcgc
    37561 ctgagcgagc tcctgcaaac ttggatagcg atcgaggaat tgaaggtact tgggaaccac
    37621 acggtcgact tgcgtttgtt gcagcatcac ttcggagacg agaatgtggt agggatcgga
    37681 tgtttttcgc caggggagat cgcggccatg gctggcgtac cacttcaaca agcgctgctg
    37741 gaaccggcgt tttaggatcc ggtccggcca ccgcgaggga cggggggttt tcttccgacg
    37801 aaaagatggg ttcgtgccaa gggccatggt aacgttgttg cggcggcatt gtaggagtct
    37861 gcacggacaa aatcaaacgc ggcaaccccg cagagattcg acgcccttac tcaccctggc
    37921 gaaacaatcc ctctcatggt aacgtgttgt tggaagcctc aaggaatcat cctatgactt
    37981 ttacggcctc ccgctccccc tggcagcccc tgttggtctt cagtgtgctg ctcgccggcg
    38041 tcgacctctt gagtgtgaac aaccgggcca ccgcgagtcc tcaacaagtt ccttgggaat
    38101 gctcaaatta cacggacgaa gcacagaccc gctgcctcag cgccttcatc gagcgtcaac
    38161 aggaacagat cggcaaactc gaaagccagc tacagaatca gcaggatatg gtcggacagt
    38221 tgaaagggca ggtcgaacgt caaagtgcgg cgacagcaca gctccagcag caactcgccc
    38281 aaccgccggt cacgacaacc atcgttcccc caccctacct gtatccctat gccttcgcgt
    38341 atccacccgt cggcatcggt ctctatctcg ggcgtccatg gattgggccc ggttattatg
    38401 ggtactatgg ccggccgtgg ggtccgcgct tctactacta tggccatcac ggacgacggc
    38461 ggtagactgg attcggatgc acatcaccaa tctgcgccca gccggttgac gaccacttcg
    38521 gggactacag caaatctgct gaggctgcaa agatagacgg cggtttcggc aatatcgaag
    38581 ggactgattt tctcgctttg caggatgtca gactcgctgg catcgaccag gtcttccgcc
    38641 actcctcccg gacagatcgc agacactttg atatggaatg gacggccttc gtcagccagt
    38701 gctctcgtca gcgccatcac cccgtgcttt gaagcgctgt aactgccggt gccggcccag
    38761 gcctggactc ctgcgacact cgacacattg atgatcagtc cgccaccctg cttcttcatc
    38821 tgcttaaagg cggctctgca acagagaaag gttccccgga gattcacgcg gatcacttca
    38881 tcaaatgcct ccgtcgacgt ctcggcgatc ctgttgccgc caaaaattcc tgcattgtgg
    38941 acggcaacgt ccactcggcc acaatcctgc accgtcctct cgaagagggc atccacctgt
    39001 ccttcatgcc tgatatcggt ctgaatgaca cgggcatcgc cacctttctg gcggatggcc
    39061 cttgccgtct tctcacaacc ttcggcattt ctcgcggcaa cgatcaccga agccccttcc
    39121 tccccgaaac gcagagcgat ggctttgccg atgccgctac tgctgcccgt gacaatagcc
    39181 acttttcctg ttaaacgact catgacgatt taaaaactat gtcaaacata cggttttctt
    39241 tgacaaagta cttagagacc cgtagcctca ttcttgcatc aatcgtcggg gaatatgggg
    39301 tgttctgtcc tggttgcatc catccggaaa gcgctcgtta tggaccagca ctggtcgaac
    39361 aaaacgtcgg aagtttaggg tgatttgcca gtgtccgtag ctacggccat cgtatacaac
    39421 ccgggaatgg gcggttgcaa gatcgtgacg aaccaagcct tgatcgtcgg tgggatgcaa
    39481 gacgtgccag gaccattggt gcctccacta cgtctcacag ctagaattcc tggcatggaa
    39541 ttggaccgca agcggcttgc tcaattactt cagttggctg ccgtgccgct taagtgaaag
    39601 gatccaacgc gtggtcactc gcaaacatcg ccggttcgtt gtcaccttgc cgagtacatt
    39661 ggttcaacgg gaccagtttc gacacaaagg ctcggtccgt gacctttcgg ctaaaggctg
    39721 tcgtgtggaa agcttgatta gtcctttcac gggcatgcag gtttcactgc tccttcagct
    39781 tccaggtgaa tcccagcctg tcaggatcga gaatgccgcg gtccgatggt gcggctcgca
    39841 gggcatcggc atcgaatttt tggtcgtggc ggagcccgag caggctcgcc tgggccattt
    39901 cattcagcaa ttggaaaaga cggcgccagg caactagtct ccgattgcat ttcttggctc
    39961 tgctctctat tctttccctc catggagctt tcctcgctct cggccagcca gctcaaggat
    40021 ttggtgcgcg gtcttgtcga cgaccgttta cgggagttga ttggtgatcc ggatttagga
    40081 ctcgaactcg gagaagcgct acgctccaga ctcaaaactt ctctcgaaaa aggcgacagg
    40141 ctatccggtg atgagctggc tgagaagcta ggactacgct ggtaaatcga tgccctggtt
    40201 tcagctgacc ttccaggctg gcgtcgctca agaccttacc gtcgcgggga gcccgatggc
    40261 gcagcggctt ctagacaaga caaagtggat tgcatccaac atagagaatc ttcgccatga
    40321 gccgatcggc gatctgccgg gattgcataa atacgctgtt gctgactggc gaatctttta
    40381 tgagatccta cggggggagt cgctcgtgaa cattcatgct attgtccaca agagtacctt
    40441 gcagcgatga ctccgcctgc catcaggaaa gacggcccgg caggtctgcg acatgcacgc
    40501 cgaactcccc tcgcctgaca tcctcgatat agcgacgcaa ggcatccttg atcacaggga
    40561 atgcaaggtc ctgccaggga atgtcggcct tttcaaacaa ccgtgcatca agactttctt
    40621 ctccggctgc gaactcttcg gccaacagct ctccaatgaa cgtcaggtac acctggccga
    40681 tatgggggag gctcagcacc gaatgcaatc tcgtgattcg cacttttgcc ctggcttctt
    40741 caaacgtctc gcgtctagcc gcctcttcgg ttccttcacc gatctccatg aatccggccg
    40801 gataggtcca cagtccagat ttgggttcga tggcacgtcg gcagagcaaa atgcgaccat
    40861 tccactctgc gatgcatcca gccacgatct ttgggttatg gtagtgaacg acctggcacg
    40921 agtcacaaac gaatcgtggc aagttgtccc cctgagggat ctttttcgtg actgatttgc
    40981 cacattcacc gcagtaattc atgggtcgtc cacgttgctc ggaacattct ccgaatggat
    41041 agcacaaaat acagattctt tgcaccctgc ccacgtggcc tggagggcgt tcttgaaagc
    41101 gaactcgacg agctcggagt gtccgccaca atcgcgacgc acggaggcgt cggctttcat
    41161 gctgcctggt cgaccgtgta tcggatcaat ctcagaagcc atattgccag cagagtcttg
    41221 tgggaaatta gtcatgagac ctatggcacg gaagaggacg tctatcgcgc cgcctatagg
    41281 ttgaactggc cggagtggtt cgcatcttcc cgcaccatta aggtgaaagt cactgcgcaa
    41341 cgttgttctt ttcgcagttt ggattttctg acccttcgta tcaaggacgc cgtctgcgat
    41401 aaattcgttg ccctgtgcgg aaagcggccg agtgtggacc ggcgacagcc ggatatcagg
    41461 atcgacgtct tcctctcatc cgacggcgtg acattttatc tcgatacgtc cggggaaccg
    41521 cttttcaaac gcggataccg tcaggcgacg gctgaagcgc ctttgagaga aaatcttgca
    41581 gctggagtgc ttcggctggc cggatggacg cctgatgaag tgctcatgga cccgatgtgc
    41641 ggcagcggca ctattccgct tgaagcggcg ctcatggctc ggcgcagagc gccagggttc
    41701 gggcgctcgt tcgcctttga atatctgcgc agctatgacg ccctacaatg ggctgatgcc
    41761 cgtgaggccg ccaaaaccga gcgtcggacg gaagtaccgt taggcattta tgcctcggat
    41821 cataacgtcg ctgcgataga actggctgcg cggacgtttc aagtggcggg cgtggcgtcg
    41881 gacattcaac tgcgacagtc ggatgtcctg tcgattgatc ctccggcgga cgaaggattg
    41941 ctcgttatta atccacctta tggagtgcga ctcggcgatc ggactgacct ccgggcgttt
    42001 tatccccagt tgggagattg tctgaaacag cgcttcgttg gttggcgtgc ctatgttttg
    42061 accgctgatt tcctttttgc gaaggagatc ggactttctc cttctcgaaa gattccactt
    42121 tataatggcc ccatcgaatg tcgtctttac gagttcaaaa ttgtcagggg aaggcccaaa
    42181 cgctcacgca aatctccttc aatcacttaa gcaaattatt gtcctgatgt ggctatgata
    42241 aagtacaact cttcggatct cctgccgtct aaaacggtga ttcgactgct cctgtccgtg
    42301 agcgtcctcg ttctgcttgc tctccccgga tgcagccttg ttcaagcttt ttttgcctcg
    42361 ctcggcgtgc ccgaaggggc tgtcaccaat actccgatgc gagactcgtc ggtcatgcgc
    42421 ctagagccaa tcgtgcgtcc catgttcgat cggctgctgc aagtccctca agcggaactg
    42481 attgaggcgc attccacggt ggaaacaatg agtgcacgct ataaacggca tcttacattg
    42541 actgcgctga aaggaccttg ggaggggttc atcaatgtcg agcgacaggg attgctgctg
    42601 gcggagttgg ccgagggacg tgccgttaat ttgcctgctc tccttgatgt attggaagca
    42661 ggcatggacc ggacctctgc gttcaataga ccaatttcaa tcccggcgaa agctacggcc
    42721 caagagctca ttacgttcat gatcgaaagc ctcgaagaag cgtcgatcca tcgtgagaag
    42781 gcgctcgcaa gtctacccga ggacgaacgc cggtttctct tcagccatgc gcagaccatc
    42841 gtcgagcagt ttacgccgca gatctcgagc gtgtccgcca cgaccattgc acaagcaaag
    42901 atggatcagc gttttgctga gctgctggaa gaacaagtgg actatgccaa tctcatggcg
    42961 gcagcgcagg tcttggctcg cttggccaac gagtcctggc tccgccagct tgccggtgcg
    43021 ttcggtcagg cgttgcccag gtcggaggtt cccgtaggca tcaccggaga tatcctgctc
    43081 gcccaggcga cttcctatgg catgatcgtc attggcggac ccggaccaaa tacctacgaa
    43141 ttggatcatc gcttcgcatt gatcgttgat ctcggagggg acgatttgta ccgcggcttg
    43201 atcgcggcgt cggccgatat cgaacacggc aatgccgtga taatcgacat gagcggcaac
    43261 gacacctatg acagcgcggc gctggggctt gccactggac gcctgggcgt gggcctgctc
    43321 atcgaccagg caggcgacga cgtgtaccag cttgagatcg gttcaggcgg ggccggattc
    43381 ggaggtttag ggatcctctt cgatgccaag ggcaatgacc tgtacatggg gacccgcctg
    43441 acgcaaggtg cagctatcgg ggggttgggt ttactgtttg acgcggcggg caacgatcgc
    43501 tatgccagcc atggattcgc cctgggtttc ggcggtcctc aaggagtcgg ggccgcgatc
    43561 gatttgcaag gtgacgatga gtatcaatgt ggaaacaagt accccagcgc ttacaatgaa
    43621 gaagacgctc ccaatggcaa gcctggagat ccgatgtttc aatacgattg ttttggtttg
    43681 ggaacagggg ccggaaggcg attactcacg aagcgacagg aatggcaggc ctatgacctg
    43741 gccggggggt ggggacttct gctcgacgtt gaaggcaatg atcggtacag gagcgccaat
    43801 ttttcccagg gtcatggata ttttttcggg gtcggcgcct tcctcgatct gagcggaaat
    43861 gatgagtacg tcgcggctcg ctacggccat ggttcttccg ctcactatgg cgtcggcttt
    43921 tttacagacc gtcagggcgc cgatcattat gaatcaacgg gccccttcta caacggcggg
    43981 gttgcatggg accatgggat gagtgtgatg atcgatgcgg gaatcgagtc cgatcgctat
    44041 ggctttcagt cctccaacgg tttgggaaaa gcagattact ccggctgggg cttgttcata
    44101 gacgaaggcg gaaacgacag ctatcagaca aaggacggct acggtctctc ctctcagcac
    44161 ggcattggcg gctttttcga tctcaagggg accgatacgt atcagatggc tccgtcaaca
    44221 tctgatgcag accttcggcc aacggatgga aaggtccttc tgtatccgag cggtggattg
    44281 tttgtcgatc gttagagcgc gctaatcttg ttgccgccca ctcttccttg acttctcctg
    44341 gacacaacct atctgtccgt tacaactcat gtcagctcac cgtgtgagcg gaggttatca
    44401 cgcatgaaca gcacaaatct ttcggtgacc ttgcctgtcc ttccgatcaa gcgcaccgtg
    44461 ttgtttcccg gaattctgat gccggtcaca gtgggacgtg agcggtccgt cgcggccgtg
    44521 aatgcggcgc tgaaaacgga agacaaaatg attctcgtcg tcgcccagcg ggatccggcc
    44581 aaagagaatc caggattgga cgatctctac acgatcggca ccaaagccat tgtgaagcaa
    44641 gtagggcgcg ccgaggatgg cgtcatccag gcgctcattc aaggtatcga gcgtgtcgcg
    44701 ttgctcaaag tggaacaagc cgacccctat ctcatggttc aggcgcgccg gctcgaactg
    44761 ccgacagaca atgatcgaga ggtgcaggca cttcaacggg ctattcaaga ctccatcacc
    44821 gatcttcctc ggctggttca ggcgcctggc atcgaagagg ccaccgcggc gtttcggaac
    44881 gaagagaatc cagccacgtt ggcctatcgt gttgcgtcgc tgctgaatct taccgtcaag
    44941 gaggagcaga cattactcga aagttcttcg acgatcgaac tgctacgctc gctctatgcg
    45001 tttctgtcca aagaaataca gattcttcaa cttcgggaga agatcaatag cgatactcag
    45061 gcaaaaatcg ggaagaatca gcgtgaatac gtgttgcgcg aacaattgaa ggcgattcaa
    45121 caggagctgg gcgaagggga cgccgaagag agtgaagtcg cccatctgaa gaaacggatt
    45181 caagaggcgg acttacccga gcacgtgcga aaggaagtcg agcgcgagat tgccaggctc
    45241 tcgaaggttc cgccttcttc acccgaccat caggtcttga ggacctatct agaactcgtc
    45301 ctcgagttgc cttggaccaa ggcttctgaa gagcacatgg atctcaccaa tgtgcgccag
    45361 gtgttggaag aggatcacta tgggatcaaa gaggtaaagg aacggatcgt cgagcacctg
    45421 gctgtgttga aactgaatcc caccgccaaa gctcccattc tctgcctggt cgggccgccc
    45481 ggtgtcggta agaccagtct tggccaatcg attgccaggg cgatgggacg gcgcttcgag
    45541 cgcttcagtc tcggcggcgt tcatgatgag gctgagctcc gcgggcatcg ccggacctat
    45601 gtcggcgcct tgcccggacg gatcatacag gcgatgcgtc gagccggggt caacaatccc
    45661 gtcctcatgt tggacgaagt ggacaagttg ggtcgcgact tccggggcga tcccgcggcg
    45721 gcgctcctgg aaatcctgga tccggcgcaa aaccatacgt tccgggatca ctatctcgac
    45781 ttgccattcg atctctccaa agtgtttttc attacgactg cgaatacctt ggatacgatc
    45841 agccagccgc tcttggaccg aatggaaatc atacgggttc aagggtacag cgaaagagag
    45901 aaagccgaga ttgctcggcg gtatttgtgg ccgcgacgac tcaaagaagc gggtctggag
    45961 gcggatcatg tccagcttcc cgacggagta ttggaccgca tcattagccg ctacacgagg
    46021 gaggctggtg tccggcaatt ggagcagatg ctgggccgat tgacccgcaa gatcgccctc
    46081 acggtagcgg aacttccaga aagccatgtg cgggagaccg tcgctgtgga agaggacaaa
    46141 cttcccgact ggctgggatc ggaacggttc atgccggagg aagcgagaaa acatattccc
    46201 gtcg
//
LOCUS       DASSCQ010000221         8691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1621596, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000221 DASSCQ010000000
VERSION     DASSCQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621596"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFH05_14010"
     CDS             <1..397
                     /locus_tag="VFH05_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679386.1"
                     /translation="MDGYGKRVLVVEDDDDVRTLIGVILRETGYNVYEACDGLEAIDA
                     LKKRRYDVLLTDYHMPKMDGLELLDLTRAMWPELPVILATSDVLLTEQTPNSLLEPAY
                     AILEKPFVRAELLTLIRSAIDGLPSIKRH"
     gene            complement(724..1005)
                     /locus_tag="VFH05_14015"
     CDS             complement(724..1005)
                     /locus_tag="VFH05_14015"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET6679387.1"
                     /translation="MRVLSGMIVGSLALLVSACAETEQGKSYWLRHAVVTLAQAAEIA
                     ETNGPGRAVGVELGQSGKSVFYDVEIIDTMNKSRHLRVDAETGKIVKGL"
     gene            complement(1027..2745)
                     /locus_tag="VFH05_14020"
     CDS             complement(1027..2745)
                     /locus_tag="VFH05_14020"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET6679388.1"
                     /translation="MRFAAVAVDYDGTLATEGLVQAPTVGALEQLIASGRQCILVTGR
                     MLRELLPLFPQAELCARIVAENGAVLYRPSTREQRLLAPQASTALADALRAKGVMPLD
                     VGDSIIATVRPHEVTVMEAIRDLGLEHQVIFNRESVMILPPGINKASGLKAALEELKL
                     SPHNVVGIGDSENDHALFQASELAVAVNSAVPTLREAADWVTLRPNGAGTAEAMLALV
                     ADDMATHAKRLTRRRVTLGSSREGVSLTVSPVEENVLIAGTSGSGKSTLAHGILEQLI
                     DKGYQTCVIDPEGDYQSIEKMIMFGNSQRGPTVAEVLTALDNPSTQVIVNLVGLPLQD
                     RPSFFFELLPQLQERRNRTGRPHRVLVDETHHLLPTKWRATVASMRDLKGMLFVTVHP
                     DQLSPHVLDTVDIAVALGERPSSTLQAVAVHKGLSLPPISDAELKPGEAVLWRVAAGQ
                     TPQPFMMTVSRMERRRHRRKYAEGELPPERSFYFRGPEGKLNLRAQNLIMFRQLADGV
                     DDETWLHHLRNGDYSRWMETAIKDRSLAQVVHEVEDMPDVSAHDSRQRVASAIEERYT
                     LPATGV"
     gene            complement(2875..3132)
                     /locus_tag="VFH05_14025"
     CDS             complement(2875..3132)
                     /locus_tag="VFH05_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET6679389.1"
                     /translation="MMQLVQDWYIAREVDRDESLTQTLMEWFSHRPALRDVTVFVLDG
                     VATLRGSVASNRDRALAIELAWDAGADDIQDDLKLTRPLAA"
     gene            complement(3282..3800)
                     /locus_tag="VFH05_14030"
     CDS             complement(3282..3800)
                     /locus_tag="VFH05_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679390.1"
                     /translation="MKARRLILGSALLMLAGCQTIGGEGMPTLTRTGEVKDVVIRDVV
                     EPTSVTVNPGDEIRWINKRQGDVRVIFLNPMTENLTCQRNFGGLMGFGTKRNEYSAKL
                     SPNETASVCFRDSGEVKYVVRAASGDPIGEQNIAGTIQIGGDRAAREFPREKEEHRSA
                     QQQPEEDTIRAR"
     gene            complement(4192..4875)
                     /locus_tag="VFH05_14035"
     CDS             complement(4192..4875)
                     /locus_tag="VFH05_14035"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET6679391.1"
                     /translation="MKTTMTVIIMALFTCFLVANGPVSAEPTGSSTMGGGSMPSPEKI
                     PQDKHTDLTGGQHGVPDEYSTTPVRQGKLKEVSDSKLLNQTVTNPQGEKLGKITKVLK
                     DETTQNIEYIMFQPQDSQNARPLPWSKFQEKGDKIVLNATKEELRPNVSSKDAKDMSP
                     DLALFMEEIEQKRNEPKASGGGNERPSGDPGSLSGAATMGEDKVGSTRSAPPTPAPQF
                     ENDAQKGKK"
     gene            5583..5960
                     /locus_tag="VFH05_14040"
     CDS             5583..5960
                     /locus_tag="VFH05_14040"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HET6679392.1"
                     /translation="MIAIGKKRWAIAEGYIPPTSTGPHPQMTSHETVCLLNTGPEDAH
                     VSITIYYVDQPPVGPYRVVVPSERTRHVRFNDLSDPAPIPSDRDFASLIESDVPIVVQ
                     HTRLDSRQAENALLSTIAFAGNE"
     gene            5920..7017
                     /locus_tag="VFH05_14045"
     CDS             5920..7017
                     /locus_tag="VFH05_14045"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET6679393.1"
                     /translation="MRFSARSPSQETNDANAYVTSGPSNGVADSDFPIGWPYNPSSWF
                     ERLWLVAVALVGFAVSSYLALYQLGYFSEVWEPFFGNGSAEVLHSALSRVLPIPDAAL
                     GAAAYMADGVTGLIGARDRWRSMPWMVILFGLAVGPLGLVSIVLVIAQPVWLHAWCTL
                     CLVSALISLVMIGPAMDEVLASLQFLRRSYERHLPLWRVFWHGTHESYDDPKTIAQRS
                     ESDQTCSSLAASSKSGGLAVWTQLLVAVVGVWITALPDVMGYEGPERLNDQIVGPLIV
                     SAAVIAAAETTRAVRWVNVMLGGWLVIAPLVLQYEPLHIGVRSSLLGIVVGGLSWLSG
                     ARTQELGGGWKRLWTSRSARVVHRPDIRFWS"
     gene            7062..7262
                     /locus_tag="VFH05_14050"
     CDS             7062..7262
                     /locus_tag="VFH05_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679394.1"
                     /translation="MLTLTTGLLLLAVIVIALYAFGVFNTPYLGAFRFAFYLLLVLLA
                     LAVWYGTMNPSHEGYDPTVGSR"
     gene            7304..8389
                     /locus_tag="VFH05_14055"
     CDS             7304..8389
                     /locus_tag="VFH05_14055"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6679395.1"
                     /translation="MKSLRRGKGNSEVVVVTGASAGVGRAIAREFARHGARVGLLARS
                     QDGLDGAARDVERLGGRALALTTDVSDDQQVEEAADRVEGDLGPIDIWINNAMVSVLS
                     PALQMTAEEFRRVTDVTYLGYVYGTLSALRRMVPRDRGVVVQIGSALGYRAIPLQSAY
                     CAAKHAIQGFTESLRSELIHDGSHVRLTTVQLPAVNTPQFSWIRTRMPNHPQPVPPIY
                     QPEVIARAVYWTAHHRRREFSVGYPTVQAILGDKFIPGLLDHYLAWVGYESQQTTQPI
                     PPNRADNLYKPLPGDFGARGEFSDRAHNYSIHSWINRYRGWLALGGLGLATWWWQDRQ
                     ARKENGPRERSSSSRTSKRRAVQRFAA"
     gene            8386..>8691
                     /locus_tag="VFH05_14060"
     CDS             8386..>8691
                     /locus_tag="VFH05_14060"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6679396.1"
                     /translation="MTDLIAARTQMAMSLGFHIVFAVLGIAMPALIAVAEWRWLRKGD
                     RNGLSLAKRWSKGTAILFAVGAVSGTVLSFELGLLWPRFMERAGPSIGPLFALEGFAF
                     "
BASE COUNT         1705 a         2574 c         2443 g         1969 t
ORIGIN      
        1 gatggatggg tacggcaagc gggtgctcgt tgttgaggac gacgatgatg ttcggacgct
       61 cattggcgtg attctgaggg agacaggtta caacgtctac gaagcatgcg acggcttgga
      121 agccatcgac gccctcaaga aacgccgcta cgacgtcctg ctcactgatt accatatgcc
      181 caagatggac ggcttagagc tcttggacct cacccgggcg atgtggcccg aactgcctgt
      241 gatcctcgcg acgagcgatg tcttattgac cgagcagacg cccaacagtt tactcgaacc
      301 ggcctatgcc atcctcgaaa agccctttgt tcgggccgag ctgttgacgc tgatccgttc
      361 ggctatcgat gggctgccca gcataaagag gcactgaccc ggtctcgaac ctgctgcatg
      421 actgagaggt ggcaaggtag cgggctcatc ggtgggggaa aaagaacgaa ccatgaagcc
      481 atgtcttcgt tggcccgagt tgaggcagct tcctcgagtc cttctcttcc tgggcgccat
      541 tctgattgtg ccgtcgtgga gtgccccaac ggccgatacg gggaagccgc cggcgtggca
      601 ggctgatccg caaccgattc cccccgcccc tgttccacgt ccgccgggtg ttccaattcc
      661 gccaacgccc aatcctcctc cgcaaccgga acccatacca ccggcgccga ttcggtgaga
      721 ggattacagt cctttgacga tcttgccggt ttcggcgtct actcggagat ggcgtgactt
      781 gttcatcgtg tcgatgattt caacatcata gaacacgctt ttgcccgact gaccaagctc
      841 aaccccgact gcgcgccccg gaccgttcgt ttcagcgatt tccgccgcct gagcgagcgt
      901 cacgacagcg tgacgcagcc aataactctt cccttgctcg gtctccgcac aagcgctcac
      961 cagaagggcc agcgagccaa cgatcatgcc tgagagcacg cgcatgtttc gcctttctgc
     1021 acaacgtcac actcccgtgg ccggcaacgt ataccgctct tcaatcgcgc ttgccacgcg
     1081 ctgccggcta tcgtgtgcgg atacatccgg catgtcctca acttcgtgga ccacctgagc
     1141 cagcgaccga tctttgatgg ccgtctccat ccagcgggaa tagtctccat tacgcaggtg
     1201 atgcagccac gtttcatcat caacgccgtc cgcgagttgc ctgaacatga tcaggttctg
     1261 cgcgcggaga ttcagttttc cctccggccc gcgaaagtaa aaactgcgtt caggagggag
     1321 ttccccttca gcgtacttcc gccgatgccg tcggcgttcc atgcgagaaa ctgtcatcat
     1381 gaacggttgc ggagtttgcc ctgccgccac tcgccacaat acggcttcgc cgggcttgag
     1441 ttcggcgtcc gaaatcggag gcaggctcag gcccttatgg accgccaccg cctgtagtgt
     1501 gctgctcggc cgttctccga gtgccaccgc aatatccacc gtatcgagca catgaggaga
     1561 caattgatcg gggtgcacgg tgacgaacag catgcccttc agatcgcgca ttgatgcaac
     1621 ggttgcccgc catttcgtcg gcaataggtg atgcgtttcg tcaaccagga cgcgatgcgg
     1681 tcttcccgtt cgatttctcc gttcctgaag ctgaggcagc agctcaaaga agaaggacgg
     1741 acggtcttgt agcggcaagc cgaccaggtt gacgatcact tgggttgatg gattgtccaa
     1801 ggctgtcagc acctcggcga cggtggggcc gcgctgggag tttccgaaca tgatcatctt
     1861 ttcgatcgac tggtagtcgc cctccggatc aatgacacaa gtctggtatc ccttgtcgat
     1921 gagttgctcg aggattccat gggctagcgt cgattttccg cttcccgacg tcccggctat
     1981 caatacgttt tcttccaccg gcgacacagt caaggagacg ccctcgcggg atgatccgag
     2041 cgtgacgcga cggcgggtca atcgtttggc gtgagtggcc atgtcgtcgg cgaccaaagc
     2101 gagcatggcc tccgccgttc cagccccatt gggccgcagg gtcacccaat ccgcggcctc
     2161 gcgcaacgta ggaacggcgc tgttgacggc caccgctagc tcactcgctt gaaagagcgc
     2221 atggtcgttc tcactgtcgc caattcccac gacgttgtgc ggagaaagct tcaactcctc
     2281 aagggcggcc ttgagtccgc ttgccttatt gatccccggc ggcaggatca tcacagattc
     2341 ccgattaaaa atgacctggt gttccagccc gagatcccgt atggcttcca tcacggtgac
     2401 ctcgtgaggc ctcacagtcg cgatgatgga gtccccgacg tcgagcggca tgacaccttt
     2461 cgctctcagc gcatcagcca gcgctgtgga agcctgcggc gcgagcaacc gctgctcacg
     2521 cgtcgagggg cgatagagca ccgcaccgtt ttcagcgaca attcgggcgc acaactcagc
     2581 ttgagggaag agaggcagca attcacgcag catccgtcct gtcacgagga tgcactgtcg
     2641 gccggaggca atgagctgct cgagcgcacc gaccgtaggg gcctggacca acccctccgt
     2701 ggcaagcgtc ccgtcgtaat cgactgctac tgctgcaaat cgcatagcgc tacccggtca
     2761 gcatgaagtg ggaacacctt gcctcagcct gtccgaccaa tttgtctgca atgtgtgggg
     2821 tagtggccgg cgtcgaggct tgtcgatgcc ggccaggaag atgagcggtc tcggtcaggc
     2881 ggccagtggc cgtgtcaact tcaagtcgtc ctggatgtcg tcagctcccg catcccatgc
     2941 taactcaatg gccaacgccc tgtcgcggtt gcttgcaacc gacccgcgta gcgtcgccac
     3001 gccatccaaa acgaagacgg tcacgtcgcg taaggccgga cgatggctga accattccat
     3061 gagcgtttga gtcagacttt catcgcgatc gacctctcgt gcgatgtacc agtcctggac
     3121 taattgcatc atgtgactcc tccttacaaa acaaaatgta gctcaatcga acgacaaccg
     3181 actcgacgtt gcccatcaaa acatacgcta gagacgaagg gcattagtaa attctctagc
     3241 gcccgaccgc tcaaacccca gtagtcaaac ccgtgtttcc cctagcgagc cctgatcgtg
     3301 tcctcttccg gttgttgttg ggcagaacga tgctcctctt tctccctggg gaattcacga
     3361 gcagcacgat ctcctccaat ctgaatcgta cctgcgatgt tttgttcgcc gatgggatct
     3421 cccgatgccg ccctcaccac atatttcacc tcacccgagt cgcggaaaca cacgctggct
     3481 gtttcattag ggctcaactt cgccgaatac tcattgcgct tcgtcccaaa tcccatcagg
     3541 ccgccaaaat ttcgctggca ggtaagattt tccgtcatgg gattcaagaa aatgacccgt
     3601 acatctccct gacgcttgtt gatccagcga atctcatccc ccggattgac cgtaactgat
     3661 gtcggctcaa cgacatcacg gataacgaca tctttgactt cgccggtgcg cgttaacgtc
     3721 ggcatgcctt cgcccccgat cgtttgacag ccggccagca tgagcaaagc ggagcccaaa
     3781 atgagccgtc gtgctttcat gttctttcct ccttcagtaa agcaagtgtg acgtgtgcca
     3841 tcgtttgagt cgttcatgcc tgcagataga taacagcaga gcgctgcaag agacaggccg
     3901 aataccaccc tgtacgtccc cacagtgccc gccgccttcg tacggctcta ttttctgccc
     3961 gacacaactt tgagacaaat tacgcctgcc atgggacaaa gcattcctac ggctctgtcc
     4021 ctcagcgtat tgattctagg acggagcaaa tcttccaagc cgagccgtta ctgagcagga
     4081 tggatgactt gtggtggagg gtcgggagcg tggagatccg gcacgccacg atcgatccga
     4141 gtgctcggac cgaccgggcg taaccctgat ctgagtggca gaatagggct gttacttctt
     4201 gcctttctgc gcatcatttt caaactgagg cgccggagtc ggaggcgcgc tgcgagtgct
     4261 tccgacctta tcctcgccca ttgtcgctgc acctgagagc gaaccgggat caccactcgg
     4321 gcgctcgttc ccgccgcccg acgccttagg ctcgtttctc ttctgttcga tttcctccat
     4381 gaacaacgct aggtcgggag acatatcctt ggcatccttc gaactgacgt tcggacgcag
     4441 ctcttccttc gtcgcgttca acacgatctt gtcccccttc tcctggaatt tgctccaagg
     4501 aagtggtcgg gcgttttgcg aatcctgtgg ctggaacatg atgtattcaa tattctgcgt
     4561 ggtttcgtcc ttcaagactt tcgtgatctt gccgagcttt tccccttgtg ggttggtgac
     4621 agtctgattg agcaatttgc tgtcgctgac ttcctttagt tttccttgac gcaccggcgt
     4681 ggtggaatat tcatccggaa caccgtgttg gccgcccgtg agatctgtat gtttgtcctg
     4741 tgggatcttt tccggagatg gcatactgcc gccccccatg gtcgaggatc cggtcggctc
     4801 ggccgagacc ggtccattgg ctacgaggaa acacgtgaac aaggccatga taatcacagt
     4861 cattgtggtc ttcatgaaag ccctcctgtt attagagaaa tgccgttgaa cccattcact
     4921 agccacttcg ttcacccatt ctgtcagccc tgagccctgc cgttccctgc ggcccccctg
     4981 atccgcggca ggcatcaact cactcttcaa acattcatgc gtgctactgg cactctgttg
     5041 catagtccac cttattggtt gtgcgctctg tccggtgatg ccgggctata gacaggctgt
     5101 cacagcgtca ggacgagtac cacgtggtat tgttagcggg gaacttggac cattgatggg
     5161 cccggcctta tccccgatca ctcctgtcct gtccactggt acttcgtgag tcgtcacgat
     5221 gaatcgggtt tcagtttctc ccttcttttg tgacccggct ccggaaacca cggtcacggg
     5281 aaaggtgaga atgacggtca acccgatgat gacaccggct ttacgattta tcagcctcat
     5341 ccccgttcag aaacgtcggc ctgaatgatg gatgtgaatg cccatcgcac gtgagtagtg
     5401 caagtcatag gccgcatcag aaagacgggg gtttccctag cgttatgctg cccgcgggct
     5461 ggaaattatt agacaacttg tctaccgccg gacagaagtt ccatcacttc ctggcctgct
     5521 cgcggaccgg ttgacgtaga atcagtccgg agtaggcact tgacttgcac attagctggt
     5581 tgatgattgc aatcgggaaa aaacggtggg cgattgccga agggtatatc cctccgacga
     5641 gcacgggacc gcatccgcag atgacgagcc atgaaaccgt gtgcttgttg aacactggtc
     5701 cagaggacgc gcatgtgtcc atcacgatct actacgtcga tcagccgccg gtgggacctt
     5761 atcgcgtcgt cgtgccgtcc gagcggacac ggcacgtgcg gttcaacgat ttatcggatc
     5821 cggcgcccat cccgtcggat agagattttg cgagtctgat cgaatcggac gtgccgatcg
     5881 tggtccaaca cacaaggctc gattcacgcc aggcggaaaa tgcgcttctc agcacgatcg
     5941 ccttcgcagg aaacgaatga tgcgaacgcc tacgtgacct ctggcccctc aaacggcgta
     6001 gccgattccg atttccccat tggatggccc tacaacccgt cgagctggtt cgagcgccta
     6061 tggctggtcg ctgtggcgct cgtgggattt gccgtctcaa gttaccttgc cctctatcag
     6121 ctgggatatt tctcggaggt gtgggaaccc tttttcggaa acggcagtgc cgaggtcttg
     6181 cactccgctc tgtcgagagt gctgccgatc ccggatgccg cgctgggggc agcggcgtat
     6241 atggccgatg gtgtaaccgg cctgatcggc gcacgcgacc gctggcgcag catgccatgg
     6301 atggtgattc tgttcggatt ggcggtcgga ccgctcggtc ttgtgagcat tgtgctggtg
     6361 atcgcgcaac cggtctggtt acacgcttgg tgcactctct gtctcgtttc ggcgctgatt
     6421 tccctggtca tgatcggccc agccatggac gaagtcttgg cgagtcttca attccttcga
     6481 cgctcctacg agaggcacct tccgctatgg agagtgtttt ggcacggcac gcacgagagt
     6541 tatgacgatc cgaaaaccat tgcccaacga agtgaatctg atcaaacgtg ctcttcactc
     6601 gcggcctcat ccaaatccgg aggactggca gtgtggacac agctcctcgt tgccgtggta
     6661 ggcgtatgga tcacggcatt acccgacgtc atgggttatg agggcccaga gcggctcaac
     6721 gaccagattg tggggccgct gatcgtcagc gctgccgtca tcgccgcggc agaaaccact
     6781 cgagctgtca gatgggtgaa tgtcatgctt ggcggctggc ttgtcatcgc tcccctcgtt
     6841 ctgcagtacg agcccttaca cataggcgta cgcagcagcc tactcgggat cgtggtcggt
     6901 ggattatcct ggctctccgg agcccggaca caagaactgg gaggaggctg gaagcgcctt
     6961 tggacgagca gatcggcacg agtcgtacat cggcctgata tccgattttg gtcttgacgc
     7021 acacccggcc ggcaacgagc tagctgaggc aggaggcttt catgttgaca ctcaccaccg
     7081 gcctattatt actggcagtc attgtcattg cgctgtatgc attcggtgta ttcaatacac
     7141 cttatctggg ggcttttcgt ttcgcctttt atctactgtt agttttgtta gcgcttgctg
     7201 tttggtacgg caccatgaat ccatctcacg agggatacga tccgacggtg ggatcgagat
     7261 gatacgcatg gtcaagaagc gtagattaaa atggaggatg gctgtgaaat cacttcgtcg
     7321 cggaaaaggg aatagtgaag tcgttgtcgt gaccggggcg tcggccggcg tcggacgagc
     7381 cattgcccgc gagtttgctc gtcacggagc cagggtcggc ctgttggcgc gtagccagga
     7441 cggattggat ggcgcggcac gagatgtcga gcgtctgggc ggccgcgctt tagccctcac
     7501 gaccgatgtc tcggacgacc agcaagtcga agaagcggcc gaccgcgttg aaggcgactt
     7561 aggcccgatc gacatctgga tcaataatgc gatggtgtcg gttctgtctc cggccctcca
     7621 gatgacggcg gaggagtttc gccgcgtcac ggacgtgacc taccttggct acgtctacgg
     7681 gacgttatcg gcgctgcggc gcatggtgcc gcgcgatcgt ggcgtcgtcg tgcagatcgg
     7741 ctctgcactg ggctatcgcg ctattccact gcagtccgct tattgtgcgg cgaaacatgc
     7801 gatacaaggt ttcacggagt cgcttcggtc ggaactcatt catgacggga gccatgtgcg
     7861 gttgaccacg gtgcagctgc ctgccgtcaa taccccgcaa ttttcatgga ttcgcacgcg
     7921 gatgccgaac catcctcaac cagtcccccc gatttaccaa cccgaagtca tcgcacgtgc
     7981 cgtctattgg acggcgcacc accggaggcg tgaattctcc gtcggctatc cgaccgtcca
     8041 ggccatcctc ggcgataaat tcattcccgg gctgctcgat cattatctgg catgggtagg
     8101 ctacgagtcg cagcaaacga cgcaacccat ccctcccaat cgcgctgaca acctctacaa
     8161 gcctcttccc ggtgacttcg gtgctcgggg cgaattcagc gatcgtgctc ataactacag
     8221 cattcactcg tggatcaatc gatatcgagg ctggctggcc cttggaggac tcggcctggc
     8281 aacctggtgg tggcaagaca ggcaggctcg gaaggaaaac gggccaaggg aaagatcttc
     8341 atcgtctcgc acatccaagc gtcgcgccgt tcagcgattc gccgcatgac ggatctcatt
     8401 gccgcccgta cgcagatggc gatgtccttg ggttttcaca tcgtcttcgc ggtgctcggt
     8461 attgccatgc ccgccctgat cgccgtggcg gaatggcggt ggttgaggaa gggagacagg
     8521 aacggtttat cgttagccaa acgctggtcg aagggaaccg ctattttgtt cgcggtcggg
     8581 gcggtctccg ggacagtgct ttcattcgaa ctcggtctcc tgtggccgag gttcatggaa
     8641 cgagcagggc cgtccatcgg tccgctattc gccctcgaag ggttcgcctt c
//
LOCUS       DASSCQ010000222         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1908125, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000222 DASSCQ010000000
VERSION     DASSCQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908125"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1113)
                     /locus_tag="VFH05_14065"
     CDS             complement(157..1113)
                     /locus_tag="VFH05_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET6679397.1"
                     /translation="MRQWHLLRLLESSRGVTLDEIADRLPADYPRHGRTIRRDLEGLA
                     TIFPILNERSDGRVRWRLMDGARQVPALSFSPTELMALMISRSLLKPLAGTEIQNALD
                     TAMAKASKLLPAASIDYVQQMQSVFAVGLGPHKNYRLHRETIDRLTEAIAKKRTVQMR
                     YYSASRLRTTRREVDPYRLWYASGGLYLVGYCHLRKEPRMFAVERMRAVTLTDHPYQM
                     PLGFDLEAFVRDSLTVMRGHPIEVELRFDKATAAWAKDRIWHPTQKVTLLKNGDMLMT
                     LTVANSRELIGWVLSFGSGVRVARPHSLLDAVSSEAKKILHA"
     gene            1545..2210
                     /locus_tag="VFH05_14070"
     CDS             1545..2210
                     /locus_tag="VFH05_14070"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET6679398.1"
                     /translation="MNPSRFFHVLLPALLICLCALLLLPSQSSAEMYVAGMVGYTSPN
                     DLSNIKGTGGGSALSLSDLALQDSIAYGGKVGYYFPQIKWLGIETELYNTTPNVKQQP
                     ATVSGFGISVPVGTLPGFDLNVLTWGINAVVRYPGKTFQPYAGVGLGMMFAKAKFGGQ
                     TDSDTAPGLNALAGIRVFATDHLALFAEYKYHRASFTFPTAIGFEADYSANLFMGGVS
                     FHF"
BASE COUNT          501 a          663 c          653 g          562 t
ORIGIN      
        1 gttcggccgg cacggatgag tccattcagt ctccttcttt ctcaacagag ataccgcttt
       61 cgatgcacaa aatcaaacac cagaccagta gaaatagttg tatttacgca gccttaggag
      121 tggattctca ggggtgactt aaagatctaa gcagtgtcag gcgtgaagaa tctttttcgc
      181 ctcggaggaa actgcatcta ggagagagtg aggccgcgcg acccgaacgc cactgccaaa
      241 gctcagcacc cagccgatca gttcacggct gttggccact gtgagcgtca tgagcatatc
      301 gccgttcttc agaagggtga ctttctgtgt tggatgccag atgcggtcct tggcccatgc
      361 tgctgtcgct ttatcaaaac gcagttcaac ttcgatcgga tgtccgcgca tgacggtcaa
      421 ggagtcgcgc acgaaggcct ccagatcgaa accgagcggc atttggtagg gatgatccgt
      481 gagtgtcacg gcgcgcatgc gctcaacggc aaacatccgc ggttcctttc gcaaatgaca
      541 gtagccgacc aggtagagtc cgcccgacgc ataccagaga cgatacgggt ccacttcacg
      601 gcgcgtcgta cgcaggcgtg atgccgagta gtagcgcatc tgcaccgttc gcttcttggc
      661 gatcgcttcg gtcaggcgat caatcgtttc tcgatgcaga cggtagttct tatgtgggcc
      721 gagtcccacc gcgaacacac tctgcatctg ttgcacatag tcgatcgagg ccgccggcaa
      781 gagttttgag gccttggcca tcgccgtgtc caaggcgttc tgaatctccg tcccggccag
      841 cggcttgagg agtgagcggc tgatcatcag cgccatcaac tcagtgggcg agaagctcag
      901 tgccggcacc tgacgagccc cgtccatgag ccgccagcgg acccgcccgt cgcttcgttc
      961 gttgaggatg gggaaaatcg tcgcgagccc ttccagatcc cgccggatcg tgcggccatg
     1021 tctgggatag tcggcaggca gccggtccgc gatttcatcg agtgtgacgc cgcgtgacga
     1081 ctccaggagt cggaggagat gccactgccg catgacctga tcgttgcgag gcatgggtca
     1141 gaagagaata tccgcatgtt cgcaagaaaa catcctactc gtttgtgggg gagaatccga
     1201 cttcggcccc gaggctggcg tggtccgact gaatctgaaa ggagtctaaa ttggatcaga
     1261 tcagattcca tttcacggca cactgtatga caaaagggaa cgatccgagg cctttccgca
     1321 gagactgtca acgggctgta ggtggagcgc ctatcacacg ttacctcata caccgtgcgt
     1381 aggctattca ttccggaagg ctttgatccg gatcgataag attcggtgtg tcaacgacat
     1441 ccccttctcg cgctgatcgt tctccggcat cctacttgct caattcccac agctaagtag
     1501 ttccttgctg ctgcaagtac ttagtgatcg ataaggaggt agacatgaat ccctcgcgtt
     1561 tttttcatgt cctgttgccg gcactactca tctgcctgtg cgccctattg cttctccctt
     1621 ctcaatcttc agccgaaatg tatgtggccg gcatggtcgg ttacacctcc ccaaatgact
     1681 tgagcaacat caagggcacg ggcggcggct ctgccttgag cctttcggat cttgccttgc
     1741 aggattcgat cgcctacggc gggaaagtcg gatattactt tccgcaaatc aaatggctgg
     1801 gaatcgagac ggaactttac aatacgacgc ctaacgtcaa gcaacagccg gctactgtct
     1861 ccgggtttgg catctccgtt ccggtaggta cgctaccagg ttttgacttg aacgtcctga
     1921 cgtgggggat caatgcagtg gttcgctatc ctggtaagac ctttcagcct tatgccggtg
     1981 tcggcttggg gatgatgttt gccaaagcaa agttcggggg acaaaccgac agcgataccg
     2041 cccccggtct gaatgcgctc gctggaatca gagtgttcgc cactgatcat ctggcgctgt
     2101 tcgccgaata caaataccat cgggcgtcat tcacctttcc cactgcgatt ggctttgagg
     2161 ccgattattc agccaatctt ttcatgggtg gagtttcctt ccacttttga ggaatcagca
     2221 tgctgacgca gacagcatga ttctcttaag gaaggggact gagcattctc gtgattcgtg
     2281 atccaattcc ccgggcaccg gcgatgatga cttcttgtcc ggcccgggtg cttagcacaa
     2341 tatcctcact cgttcgtgat ggtccccgcc tcccctatt
//
LOCUS       DASSCQ010000223         4797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1908222, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000223 DASSCQ010000000
VERSION     DASSCQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908222"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFH05_14075"
     CDS             complement(<1..901)
                     /locus_tag="VFH05_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679399.1"
                     /translation="MLSSGSGHCRLRRRWVVLVTVVFAVAEAPVRSHAEWAAIAEEKT
                     SYTTDAFQFSSARRLALTEDPSQPTVVPLNKQEDVIWEPSLEVIQSSSNSLGRNDLSI
                     KAHGYIYTNNPIFNHGEYRLQDRQSLGPDTSILLRYRYVPNLFLGPNFERRTGTRSIQ
                     EERVTSHQWRAELERRLTEEWTAVLVGRYGLRLYNEAFAERDTTFYGAGPRIEYRPTH
                     WTSLTLGYLYERGLADGRDEIQFADDVSYYLNMLSVGTELRLTARVELNLVYLYLRKT
                     FTSGLAGDSHVDRRDSTHQGLAEV"
     gene            complement(905..1348)
                     /locus_tag="VFH05_14080"
     CDS             complement(905..1348)
                     /locus_tag="VFH05_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6679400.1"
                     /translation="MRTLIAAIPLVTCVVGLPVDPTIASDAAVLQPRVPSDQIDEART
                     WKNVHEATPENVEKGKAIFHGKAFCVTCHGRDGRGLGDIPGLVGRLPRNFTDKAWQAA
                     RTDGELMWVLKNGSPGTDMASFIPLILTEEEAWYVLLYVRSFGQP"
     gene            complement(1415..1939)
                     /locus_tag="VFH05_14085"
     CDS             complement(1415..1939)
                     /locus_tag="VFH05_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HET6679401.1"
                     /translation="MPRCFRSLSVTSAAAFFLLICTTLPASAAEPASPVEGFDIHVQA
                     PHLMPNGEPGGPFHHYCKGISDKILQCLLFESTDPKAKLVAIEYFVSKDLTRKLPAIQ
                     WHRHFHDHKVEIATGRVQVLDMPAEQAAKVAEAASATDGVIYHLWQKGLEFPDGSVSF
                     PQSLGHTFPGHAEK"
     gene            complement(2146..2565)
                     /locus_tag="VFH05_14090"
     CDS             complement(2146..2565)
                     /locus_tag="VFH05_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET6679402.1"
                     /translation="MSIRSHSAASLFVLLCVFGLSTAIPVESQSEQVVEVVIKDFTFI
                     TKQEVLRLGLPTVIKIVNKDGERHDFGSTMFEGLQARIEKDGVIVYGRGLGGVMLDPK
                     RDATIRFNMERPGRHTFRCSIHPNMKGELLLLSTEAV"
     gene            complement(2686..3330)
                     /locus_tag="VFH05_14095"
     CDS             complement(2686..3330)
                     /locus_tag="VFH05_14095"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6679403.1"
                     /translation="MHSSAYTTTFTTLLHLYASMTKRITIIQGHPDSDSRHFGHALAD
                     EYANGARDSCYDVRKVDVGQLDFPLLRTKGDFEKGALPVGLAQAVEAIKWANHLVIIY
                     PLWLGSMPALLKGFFEQVFRPGVAFEYQPNGKMPRKLFAGKTARIIVTMGMPAFLYRW
                     FFFAHSLKSLKRNILEFCGITPVKATLIGNVEGLRPEQRTTWLDNVRKLGEMSK"
     gene            3496..4665
                     /locus_tag="VFH05_14100"
     CDS             3496..4665
                     /locus_tag="VFH05_14100"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET6679404.1"
                     /translation="MRIFVLGVGATGSLLMKLLDRHGHHVSCGDRDPVRARAFLGEKA
                     IFPIHRVNARNVFDIVKAAKGCHLLINTCPAVVNKIVLRAALRLRVHYLDTASQLRQH
                     PFRPEQLDFDHHFRRKRRTALIDAGAAPGLTNVLAMHASTAFEPLDRIQIRLFEGTSS
                     DHPVSQWSPDSSFDEAVSRPRLYRNGHFQFGKKFGEREMFRFLPPIGRVGVMLAAQDE
                     VVTLPNALRMDEMDAKIGGPDMDRFRRWYRQGKLNRSRGIAAVRFPATPSPRVISRLV
                     RRGLLRNARFAVAVLIYGRIHGRPMLVRWDATFPSLVDLRHRGLSCSPIAWANAQLAA
                     IFVKHFPRQQTGVVTPESLPATVLKCILRDVRSRGIRLTKKAMRQKISEGIDKEV"
BASE COUNT         1064 a         1284 c         1335 g         1114 t
ORIGIN      
        1 gaacctcggc gagcccttga tgggtggaat cgcgacggtc gacatgcgag tccccggcca
       61 ggccgctcgt gaatgttttc ctcagataga gatagacgag attcagctcc acccgggcag
      121 tcagccgaag ttccgtcccc accgaaagca tattgagata gtacgacacg tcatcagcga
      181 actgtatttc gtcacgacca tcggctagac cgcgctcata caggtacccg agcgtgagac
      241 ttgtccagtg cgttgggcgg tattcgatcc gcggccctgc gccatagaac gtggtgtctc
      301 gttcagcaaa agcctcattg tagagccgga gcccataccg tccaacaagt acggcggtcc
      361 attcctcggt gaggcgccgc tctagctcag cccgccactg atgcgaggtc acccgttctt
      421 cctgaatcga tctggttccg gtgcgccgtt cgaagtttgg gccaaggaag aggttgggta
      481 catagcgata gcgcagcagg attgacgtgt cgggaccgag cgactgtcga tcctgtagtc
      541 gatattcgcc atgattgaaa atagggttat tcgtgtaaat gtacccgtgc gccttgatcg
      601 agagatcatt gcgtcccagg gaattggagg aggattggat gacctccagt gatggctccc
      661 atatgacgtc ctcctgcttg ttgaggggca caacggtcgg ctgggaagga tcctcggtaa
      721 gtgccagacg tctggcagat gaaaattgaa acgcgtccgt cgtatagctc gtcttttctt
      781 cagcaatggc tgcccattcg gcgtgggacc ggacgggagc ttcggccacg gcgaatacga
      841 ctgtgacaag gacgacccaa cggcgtcgca gccggcaatg tcccgagccc gacgagagca
      901 taggttatgg ctggccgaaa gatcttacat acagcagcac ataccaggcc tcttcttcgg
      961 tcagaatgag cggaatgaac gaagccatgt cggttcccgg actgccattc ttgaggaccc
     1021 acattagttc tccgtcggtg cgagcggcct gccaggcttt gtctgtgaaa ttgcgcggaa
     1081 gcctgccgac gagaccgggg atatcaccga gcccccttcc atctcttcca tgacacgtga
     1141 cgcaaaatgc tttcccgtga aaaattgctt tgcccttttc gacgttctcg ggtgtggctt
     1201 catgcacatt cttccaagtt ctggcctcat cgatttgatc ggaaggaact cgtggttgta
     1261 ataccgccgc atccgaagcg atggtcggat cgaccggtaa tccgactacg catgttacca
     1321 gtggaatagc cgctatcagc gtcctcatcg ttccggcctt cgatgatcga actgccccca
     1381 atgacgggag cagttctcca tcttcgcacc ccacttactt ttcggcatgc cctgggaacg
     1441 tatgtccgag agattgcgga aaactgacgg atccatctgg aaactccagc cccttctgcc
     1501 acagatgata aatcacgcca tcggtggcgg aagcggcttc tgctaccttc gcggcctgct
     1561 cagccggcat gtcaagcact tgaacgcgac cggtggcaat ttcaacttta tgatcgtgaa
     1621 aatggcgatg ccattgaatc gccggcagtt ttcttgtgag gtctttggag acaaaatatt
     1681 cgatggccac gagtttggcc ttgggatcag tcgactcgaa caagaggcac tggagtatct
     1741 tatcggagat gcctttgcaa tagtggtgga aaggcccacc cggctctccg ttgggcatca
     1801 gatgaggagc ttggacatgg atatcgaatc cttcaaccgg tgacgcaggt tcggcagcgc
     1861 ttgcgggcag agtcgtacag ataagcagaa agaaggcagc tgcgctcgtg acactcagtg
     1921 aacgaaagca tctgggcata agaacctcct tggggcataa gggttgtgat ttaaacgcgc
     1981 acgccctgta tgaggcaggt ggccggtatt ggattcatac atttgcctgc gccctgcatc
     2041 gggcccggat agccgtgtga cgatcaccgg agcgagctga tgctgcacga ttgcatccgt
     2101 ccgtgcatcc atggcttcgc tgacaatcca aaggccaaca gccatttaga cggcttccgt
     2161 actcagcagt aacagttcac ccttcatgtt tggatggatc gaacagcgaa aggtgtgccg
     2221 tccgggtcgt tccatgttga accgaatcgt cgcatctcgc tttggatcca gcatgacccc
     2281 cccaaggcct cgtccataga caatgacccc gtctttctca atcctagctt gaagaccctc
     2341 aaacatggtg gacccaaaat catggcgctc gccatctttg ttcacgattt tgatcacggt
     2401 tggcagccct aaccggagaa cttcctgctt ggtgatgaaa gtgaagtcct tgataacgac
     2461 ttcgacgacc tgctcggatt gcgactcgac gggtatggcc gttgataggc caaagacaca
     2521 cagaagcacg aatagagatg cggccgaatg tgaacgaatc gacatgaaaa cctccttctc
     2581 gttattgacg cctagtttgc cgactgttgc cggcagtgac tgttccggct tgattcgctt
     2641 ccgaggacag ataagaagtg cgacagatca tggctacggg aaaggtcatt tggacatttc
     2701 accgagtttt cgcacgttgt ccagccatgt tgttcgctgt tccggccgca agccttcaac
     2761 atttccgatg agtgtcgcct tcacaggagt aatcccacag aactccagga tattgcgttt
     2821 gagacttttg aggctatggg cgaagaagaa ccaccgatac aggaaggctg gcatgcccat
     2881 ggtgacaatg atgcgtgctg tcttaccagc aaagagtttt cttggcattt tgccgttcgg
     2941 ttgatattca aacgccactc caggccggaa aacctgctcg aagaacccct ttagaagcgc
     3001 cggcattgaa ccgagccaga gtgggtaaat gatcacaaga tgattggccc acttgattgc
     3061 ctccactgcc tgtgcgagtc caaccggcaa agcgcccttc tcgaagtctc ccttggttcg
     3121 gaggagagga aaatccagct gcccaacgtc tacctttcgc acgtcataac aactgtctct
     3181 cgcgccattg gcatactcgt ccgccaatgc atggccaaag tgacgggagt cggagtccgg
     3241 atggccctga ataatggtaa tgcgcttcgt catcgaggcg taaaggtgga gcagagtcgt
     3301 gaaagtcgtc gtgtaagcag aagagtgcat tgtcggtgcc agaaccgcat ctgaccgctc
     3361 gggatgagtt tattcagcaa atgccgtatc ttaagctccg tgatggacgt ggacatattc
     3421 gctcccatac gcatctgtag cgttatgcga caacgacatc cttaaagccg acctgttgtg
     3481 atacattgcg gcccgatgcg gatttttgtt ctgggagttg gcgcgacagg atcattactg
     3541 atgaagcttt tggaccgtca tggccatcac gtgtcctgtg gcgatcgaga ccccgttcgt
     3601 gcgcgcgctt tcctgggcga gaaagccatt ttccctattc accgtgtgaa cgcccgtaac
     3661 gtcttcgaca ttgttaaggc ggcgaaagga tgtcaccttc ttatcaacac gtgtccggcc
     3721 gtcgtcaaca agatcgtact gcgtgcggct ttgcggttgc gcgtgcatta ccttgatacg
     3781 gcttcacagc tgagacagca tccattccgg ccagaacagc tcgattttga ccaccacttc
     3841 cggaggaaac gtagaacggc actcattgac gcgggtgcag ccccgggctt gacgaatgtc
     3901 ttggccatgc acgcttcgac tgcgttcgaa ccacttgaca gaattcagat tcggctgttt
     3961 gaagggacat cgagtgatca tcccgtttca cagtggtctc cggattcttc ttttgacgaa
     4021 gccgtttcga gaccgcgact ataccggaat gggcattttc aattcgggaa gaaattcgga
     4081 gagcgtgaaa tgttccgctt tttgcctcca attgggcggg tgggagtcat gttggccgcc
     4141 caggacgaag tcgtcactct cccgaatgct ttgcgaatgg acgagatgga cgcaaagatc
     4201 ggcggcccgg acatggatcg gttccgtcgc tggtataggc aggggaagct aaaccgttcg
     4261 cgtggaatag cagcagtcag gttccccgcc actccatcac ctcgtgtcat cagccggttg
     4321 gttcgaagag ggcttctccg aaatgcccgc ttcgccgtgg cagtgttgat ttatgggcgg
     4381 atacatggca ggccgatgtt ggttcggtgg gacgcgacgt ttccatcact ggtggatctc
     4441 cgccaccgcg gattgtcctg ctccccgata gcatgggcga acgctcaact ggcggctatc
     4501 ttcgtaaaac actttcctcg gcagcaaaca ggggtcgtca ctccggagtc gttgccggcg
     4561 acagtcctca agtgcattct tcgggatgtg cggtctcgcg gaatccgtct cactaaaaaa
     4621 gccatgcgtc agaaaatatc cgaggggata gacaaggagg tgtagtcgat agcacttggc
     4681 cattgcggga gcgccaacat tcgccatgcc gtgagtttgc tgaaggctct ttgggaaatg
     4741 gccaatacgt gatgacggtc tgatcagttg gtcaatcgat agacgagtat cggatag
//
LOCUS       DASSCQ010000224        24184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1107203, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000224 DASSCQ010000000
VERSION     DASSCQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 24184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107203"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..878
                     /gene="tadA"
                     /locus_tag="VFH05_14105"
     CDS             312..878
                     /gene="tadA"
                     /locus_tag="VFH05_14105"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET6679405.1"
                     /translation="MQSITRDALVPAGLTPPGKLATTGAMIDPATTSDSRFMRAALEQ
                     AACAAALGEVPIGALLVHDGAIVAQAHNFRERWQDPTAHAEMIVIREAAKALGRWRLI
                     DTTLYVTLEPCAMCLGAVILARIPRLVFAAKDPKAGACGSVLDFASHPRLNHRVTVLG
                     GILEGESQRMLAEFFKAIRAQDVDGGTL"
     gene            complement(885..1679)
                     /locus_tag="VFH05_14110"
     CDS             complement(885..1679)
                     /locus_tag="VFH05_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET6679406.1"
                     /translation="MISRSLQTVLAFPPVDPPTPATQVATVVLDDQTVDVWSFALVHK
                     TSVLQRCQAWLSEQERARAQRFIHAAQGNDFILAHGALRGVLARYLAVAPETIEFDVG
                     STGKPILLNASGSSHSLRFNLSHYHGRMLVAVAKGREVGIDLEQVRETLDALKLGERF
                     FAAAEYEDLKNRTEPDRTWQFYRYWVAKEAVLKGQGIGIESLQRCEILPDGCGPRPSA
                     TVRCLDSSARQGGWTIQWLNCGAGWQGAVSADGSDWTVRIMDGETP"
     gene            1831..1920
                     /locus_tag="VFH05_14115"
     tRNA            1831..1920
                     /locus_tag="VFH05_14115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1865..1867,aa:Ser,seq:cga)
     gene            complement(2321..3961)
                     /locus_tag="VFH05_14120"
     CDS             complement(2321..3961)
                     /locus_tag="VFH05_14120"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HET6679407.1"
                     /translation="MSDTVGHQPILTEAVLSGLDKHGEHQLGFADMTTFPEPDRLPPI
                     APRLSKSKFLSGLQCHKRLYLEIRRPELATPPDPSTQAILAMGTEIGVLARRRFPGGV
                     VVWPVPRQREAAIFQTAKLLEDSRIPAIFEGAFEHEGVVVRVDILERIPVADGTSPAW
                     RLIEVKSATRVKDVHLDDLAIQRHVLLGMGLNVAETCLMHINTGYLYQGGEIDLQALF
                     AIDEVSAAVVDRQGKVPEQLLAMKAVVSEPHPPAIEPDARCHSPYECQFWAHCTQDKS
                     PRWIYRLPGVKKMVEGLVQNGITTIDDIPDDVNLSITQRRVKDNVEWISTGLASVLRS
                     VVYPVHHLDFETVMLAVPRFPAMRPYQAIPVQWSNHIELESGEIIHQEFLHGEATEPR
                     KRWAETLIESLGDAGTIVVYSSYEEAMMRQVAEAFPEFRQALSAMTRRLWDLLPVIKE
                     HYYHPGFQGSYSIKFVLPAIVPGMSYRDLLIQDGGQAASEYYRMVFVEEDWIEQLKIR
                     EALLQYCARDTMAMVELRRVLMKKAATLSSQASSEVTS"
     gene            3941..5266
                     /locus_tag="VFH05_14125"
     CDS             3941..5266
                     /locus_tag="VFH05_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET6679408.1"
                     /translation="MANGVTQIRRSVMALALPVTVSSLLQRTEGIVAVFLVGGLGAVP
                     IAAVGLGQLLAFIASTLVSGLSVGTNVIVAQLWGARRRHDAGQAARHFLALSIAVSLV
                     LMAGGVSLNHFAMRLLGAAPEVIALSLPYSTLIFLVIPFTVLLQVLSSILQGTGNTKT
                     PMYAMIVVNVLHILIAFPLIYGHWGFPALGVQGAAIAVGIAESAGTLYLGLRCLPLLQ
                     RSQRVRWDLFRTMWQVGAPVSGERIVQQAGILAYTKIVLLYGTVSYAAHQVGLSIESL
                     SFLPGYGFAIAAATMVGQSIGARKYTRAKLENWEANRLAIGIMSAMGLVFFFFPYALL
                     RAFTSDESVIELGTLFLKIVALIQIPLALTMVLAGSLRGAGDTHFIMVATTIGMWAVR
                     VPIALITGVWLHLGIFYVWVAMIADWTLRMGLMLWRYRSERWKAIQVIR"
     gene            complement(5267..6634)
                     /locus_tag="VFH05_14130"
     CDS             complement(5267..6634)
                     /locus_tag="VFH05_14130"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HET6679409.1"
                     /translation="MNNILMVGAGSVGGFFGAHLAQHHPGVSFLLRPKTLAAVQKNGL
                     QVRSASGTVTVHPRAATDARQLPQPELIILAVKAYDLDEVLTQIEPVLTERTVILTLQ
                     NGIDTEDRIIERVKRDCVVGGVAYIYSKIVAPGVIDHYKKGNVAIGELMGHESARLLE
                     IRDLFASAGLPCQLSKDIRRAKWEKMCWNCVFNPLTVLVDDKVSAALDHPEMMHVIRQ
                     IVQEITAVSAALKVPLPSDMSDRVVRWSQEIRDIHTSMYDDWKAGRQTEIRNLNGYIV
                     ERGRELGIPTPVNEALTAMIKTVTEKPRTGPGIVEIDGAVVQPVLFDRMALRQLPAEC
                     QVSDVNTLMPSMRGRAITVKGLLEIPALAIAADHVTFHSNDGNYAATLTLQQARDFGL
                     LLYEVDDQPLPETKGGPYRLITPGMGDLCANVKAVRRIEVRIGSGKDTRPSERPSVCT
                     ESKRS"
     gene            6720..7445
                     /locus_tag="VFH05_14135"
     CDS             6720..7445
                     /locus_tag="VFH05_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET6679410.1"
                     /translation="MTSGSDLDLLKRKLQSARSLAILTGAGISADSGVPTFRGPDGLW
                     RNFRAEDLATPEAFARDPRLVWEWSNWRRELIATKSPNPAHVAVAEFERRFEEFWLIT
                     QNVDGLHRAAGSEKLSEIHGNIWKVRCTACGTVEDNHDVPIAILPTCRHCQGLLRPHI
                     VWFGESLCPDDLACCAAALRACEVLLVIGTSGVVYPAAGFTSVAKDTGAFVAEINFES
                     TPQSGTMDLTLKGRAKDLVPLLL"
     gene            complement(7480..8202)
                     /locus_tag="VFH05_14140"
     CDS             complement(7480..8202)
                     /locus_tag="VFH05_14140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6679411.1"
                     /translation="MSQSIQVVLFDAAETLFHVNGSVAEIYLRYAAEFGYPRKADSLQ
                     SITEAFRRAFRDAPPPVFAATESAQIKQSERLWWFDVVHNVFYRVGMFERFDEFFERV
                     FDVFADPSSWKLYPEVVPTLKELRSRGLELGIVSNFDSRLFNVLRGLGIAEYMDTVTI
                     SSLAHAAKPSPKIFELALEKHAVEAADALHVGDSLTDDVEGARKAGIRAVLLAREGKQ
                     PPPPGIAAIRALDDLLPLIAPH"
     gene            8343..9494
                     /locus_tag="VFH05_14145"
     CDS             8343..9494
                     /locus_tag="VFH05_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679412.1"
                     /translation="MPSTIFVIDSSPAVRRMVEQISTPEGFDVVGFQDGPAALEAARR
                     MSPALIITDYHLDSMTFSGFCKEVHKLDNLADTYIIALTGATDKLENTHLKALGVKAF
                     LKKPFQSEHLLDLIKDLDRTNTTNISGKKKRSWPPTSSALDSDDECAGDSADAMSDEE
                     LHPIIPMTLDVTPVKKEAMKPPVPESASTPPAAEPEEAMKGLFGQLLESMSGKTEKKI
                     ADMLPHMVNTGLEAQVSRAVEAEVRLQLGAAVSQERLTEIIEPLVMQALPKILTREMA
                     VLEPIIRHSMVEIASPLIKEHIEHMVREQAETVRQSLQDAVREHLRSVEEEVRAQLKE
                     AATAETKKLATDVVRTIAEDQIRTSVQMVAPSIVEEQIRAEIARLTEAA"
     gene            9779..12547
                     /locus_tag="VFH05_14150"
     CDS             9779..12547
                     /locus_tag="VFH05_14150"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET6679413.1"
                     /translation="MAPLQLDKTYDPKAVESRWYQFWAERQYFHADPSHPGRPYSIVI
                     PPPNVTGSLHVGHALNHSLQDILIRWRRMQGRNTLWLPGTDHAGIATQNVVEKQLLGE
                     GISREALGRDGFVERVWQWKAASGNRIIEQQKQLGESCDWERLRFTMDAGLSKAVLEV
                     FVRLYEDGLIYRGERLINWCPRCLTALSDIEVEHEDVKGTLYHIRYPLTDDPSTSLTV
                     ATTRPETMLGDTAVAVHPDDPRYRHLIGKSIRLPLTQRTIPVVGDAILVDLEFGTGAV
                     KITPAHDFNDYEAGERHHLPRLPIFNHQALLDPALMKTAGVDADVISAVASLPVSKAR
                     PKIEQALKDRALLSKVEDHRMALGKCYRCKTVVEPYLSPQWFVEIKPLAEPAIRAVEE
                     GRIRIIPEGWVNNYLGWMRDIKDWCISRQIWWGHQIPAWYCERCNEPFIHRQTSTNQA
                     GDVVTRITLLQGAKPVVARSTPAKCPQCGGEQWLRDPDVLDTWFSSGLWPFSTLGWPE
                     QTPELKTYYPTATLVTGLDILFFWVARMIMLGLRFMNDVPFRDVYIHALVRDAEGQKM
                     SKSKGNVIDPLHVMDQFGTDALRFTLASMASPGRDLKLAEERIEGYRNFANKIWNAAR
                     FGLMYLDGARTAVRPSERPFPDRWILSRLNHTIQTVTSELEAYRFDRAASALYQFIWH
                     EFCDWYVELIKPVLQNPSHADGPVTRQTLVETLEVTMRLLHPFMPFITEEIWQTIPHE
                     GESIVIRPYPASEQTWDDPVTEEQFLLLEQTVGLVRTGRVLLNYPPGQPIEFSMTHDD
                     PAKLRQLEHLQPHVSHLSRGTADMLSNGVSPVARRLRLVAGGLSIALVIAGDVDLKKA
                     LDRIVKQREQQEKELTRLEGKLNNHEFRGKAPADVLAEHESRLRSLQNDQALLASSER
                     QLRALLGP"
     gene            12550..13425
                     /gene="nadC"
                     /locus_tag="VFH05_14155"
     CDS             12550..13425
                     /gene="nadC"
                     /locus_tag="VFH05_14155"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET6679414.1"
                     /translation="MPELPAADIRRAVRLALEEDLGQGDVTTSALFPSAVPARARIVA
                     QQPLVVAGMAAAVQTFLAVDASLRLAVLKKDGAQALNRETLLRIEGDGRSILMAERVA
                     LNFLQQLSGIATLTQRFCQAVDGRVAILDTRKTIPGWRSIQKWAVALGGGTNHRRSLA
                     DGILIKDNHLKLLRHKQPVFQSCTLARQRGPAGMPVIVEAESLKEVAQAVAGQADIIL
                     LDNMTPAMVKKAIKIIDGRALVEVSGGITLDNVRAMAAAKPDRISIGALTHSAPAATI
                     TLELERAVQGRQQRR"
     gene            13429..14262
                     /locus_tag="VFH05_14160"
     CDS             13429..14262
                     /locus_tag="VFH05_14160"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET6679415.1"
                     /translation="MKHTSPLTVDDIERMLATRSLGRRIELRDRIDSTNREAMALGQA
                     DAEHGTLVLADSQTAGRGRLGREWFSPPGINLYASIVIRTTLDTQRLSQWLSWLPLMT
                     ALGTAEAIESAGAARVSLKWPNDLLIGDRKVGGILCESGASVRSGPFQVIGIGINVNG
                     ERASFPRGLRDFATSIRQETGREIDRHRLLVRLLEELELCLEEFLTRGIDRLAWEYRR
                     RCTTLGKTVKAVLADGNEYVGTAEGIGDDGSLTMVHEAARPGGQSEVRQLRAADITHL
                     R"
     gene            14308..15096
                     /locus_tag="VFH05_14165"
     CDS             14308..15096
                     /locus_tag="VFH05_14165"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET6679416.1"
                     /translation="MLLVADIGNTNVVLGIYDGQVLKAHWRLATDAKTTADEYGILFT
                     NLLASAGFLPRQVSGAIMSSVVPTLTATFETLIEQYFHQRPLIVTSETDTGLTIRYPN
                     PKEIGSDRLVNAAAAYHKYPRDLIIVDFGTATTFCAVTKEAEYLGGVIAPGLGISAEA
                     LFTRAAKLSKVELARPKTIIGTDTGSSIQSGLLFGYAGLVDTLVKRIEHELGRSTSVI
                     ATGGLSTIIASEATSIQIIEPLLTLDGLALLYRRANGSAPTAWV"
     gene            15186..15761
                     /gene="grpE"
                     /locus_tag="VFH05_14170"
     CDS             15186..15761
                     /gene="grpE"
                     /locus_tag="VFH05_14170"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6679417.1"
                     /translation="MTDESLNTNTFDNLDSDSGGPASSGDSLAESAARDGELKTLNDK
                     YLRLAAEFDNYKRLAQRDQRDQIRFGNEQLLKELLPVVDNLERAIKAAKDSKTAQGLV
                     QGVDLTLKQLESVLSKFGVQSIPTMGQRFDPSGHQAVASVPSTQVPDQHVVEEFQRGY
                     RLHDRILRPAMVTVSSGAGSGSDGDEIDNSN"
     gene            15847..17775
                     /gene="dnaK"
                     /locus_tag="VFH05_14175"
     CDS             15847..17775
                     /gene="dnaK"
                     /locus_tag="VFH05_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6679418.1"
                     /translation="MGKVIGIDLGTTNSCVAVMSGGDPVVIANAEGARTTPSVVAITD
                     KGERLVGQIAKRQAITNPENTIFSVKRLMGRKFRSKEVQEAMKRLPYKVVEADNGDAH
                     VELRGKRYSPPEISAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERISVYDLGGGTFDVSVLEIGEGVFEVKSTNG
                     DTYLGGDDFDLRVMDWLVDEFRKDQGIDLRKDRMALQRLKEAAERAKIELSSSQETEI
                     NLPFITADASGPKHMVIKLTRARLEQLVDDLIQRTVEPCRKALTDASVSARDINEVVL
                     VGGMTRMPKVISVVKDFFGKEPHRGVNPDEVVAIGAAIQGGVLKGDVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTVPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPSAPRGMPQIEVTFDIDANGIVHVSAKDLATQKEQSIKITASSGLSKDEVE
                     KLVRDAQTHTEEDKKRRKAAEAKNQADNLLYQTEKNLKEHGDKISADDRTKIEEAVTA
                     LKKAMEGTDPDAIESATQALTTASHKLAEEMYKKASASAATGAGTDTGGGAESPNGAK
                     TDEKVVDAEFEEVDKDKK"
     gene            17854..18978
                     /gene="dnaJ"
                     /locus_tag="VFH05_14180"
     CDS             17854..18978
                     /gene="dnaJ"
                     /locus_tag="VFH05_14180"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6679419.1"
                     /translation="MASVSKRDFYETLGVERTASEEDLKKAYRKLARQFHPDLQTGEQ
                     QKKHAEEKFKEINEAYEHLSDQEKRKRYDMFGHAGTQAGSGFEGFDFGRGGFGDVFND
                     IFEDFFGGQRGGNRAERGNDLQYNLEITFEESVYGKEAKLKIPRWETCSECKGSGAKS
                     ASSVKTCPSCKGAGQIRLQQGFFSVSRPCGQCEGSGHIITEPCTACQGRQRTYRERTI
                     SVHIPAGIETGMRLRLSNEGEHGINGGPAGDLYVAITVKAHPIFQRKGNDIVCDVPIN
                     FVTAALGGKIEVPTLKGTTVMKVPPGTQPDKVLRLKGLGIPSLKNQTTGDQLFTIKVH
                     IPTKLTAKQRELLTEFAKESGMSMEADGDGFFDKMKTFFE"
     gene            19010..19750
                     /locus_tag="VFH05_14185"
     CDS             19010..19750
                     /locus_tag="VFH05_14185"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET6679420.1"
                     /translation="MPTFFVAADSVVPPTVRITGPLLHHLRQSLRLQPGETLTVTDDG
                     GRRYRTEVGEITAGHLTARILETTTAPPKTSPSIVLAQALLKGEKMDWIIQKATELGV
                     DRIAPVVTTHGVVKPRAERLDHQRARWQRIALEAAQQSERWSVPTIDEPATLSQVMTA
                     YDASAIKLFLAERSSGTSLTGMAAGLGAEHAILLVVGPEGGWDKDELRFAQEQGCQAI
                     TMGKRILRAETAALAAISVLQSRFGELG"
     gene            complement(19753..20715)
                     /locus_tag="VFH05_14190"
     CDS             complement(19753..20715)
                     /locus_tag="VFH05_14190"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HET6679421.1"
                     /translation="MTILLFVPLSLLLITFIISPTRAAESQLKAAPQKTGTEVTLMSV
                     YFRDANLGWAVGSGGTVVKTVDGGQKWKKQTSGTSVQLTGVFFVDDKQGWIIGANGTI
                     RHTTDGGSSWRAQPIETVAPMYGLSFVSPDEGWVVGGNGTILHTTDGGAHWTDQSSPT
                     TAALYAVQFLTPKRGTAVGAVGTVLATEDGGTTWLAQETQGSVTLFDVYFSDPMTGWV
                     VGNAGAMFQTTDGGGRWVDRTLPCSRTCTRLTDLLRIRFTDPKTGWIVGERGMAYHTA
                     DSGFTWEEQGAVANVSLFGLSFPTTLHGWASGEKGTVVHLKLSK"
     gene            complement(20828..22525)
                     /locus_tag="VFH05_14195"
     CDS             complement(20828..22525)
                     /locus_tag="VFH05_14195"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET6679422.1"
                     /translation="MMVKTYLSRWIIVFGTVLLVALGSDQGTDASPSARLELQPATGV
                     PGATVSIVGKGLGHFKSAQVNRVLFRGVPALIQRWDSDVVEVKVPFKAQTGPVEVLIG
                     KKKLAAGTFSVVYPKILSIHPPAIEPGHAVEIVGEHFGITAGPRDPNTMFGVNDVMVG
                     NLVVRPRRWKDDKIEVDVPANATSGDIAVRLASSDPLPDGSCCAPVQHTVSNAVSLKL
                     IPTVQVDPLTGPVGTKVVLFGQGFGQERAAGDLITIGGHEAPVAQWSDRAIVVHVPLN
                     AETGPVVLSRHGQDRTLGTFTVQTPKATGLSPASAPIGTLVRIHGEHFGAYSESGATP
                     YNYTDFDTGQNRVEIGGAPAVIYRWHDDRIDVWVPFSAKSGPVVVTRGAPKPAADGSC
                     CSERGTVSTTAGTFTLVVPTIESFTPASAGLDEVVTIKGSGFGSFIKTTEATQPGLGE
                     GAFKRNQIELGENVSRTEVLFNNIGAIVVSWTDTEIKARVPHRHLFGIGKPGQFNADL
                     STGPLIIRRGSWDLLPDGTCCTPKKWVTLEAGTFTIEAKGMPDQSFFQSRPEGSTND"
     gene            complement(22684..23142)
                     /locus_tag="VFH05_14200"
     CDS             complement(22684..23142)
                     /locus_tag="VFH05_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6679423.1"
                     /translation="MTYEEKLTSLGLELPEAPKPVATYVPAVRTGNLLFLSGVLPMRN
                     GQLAFTGKLGRDLTVPEGVEAAKVAILNALAIAKQELGSLDRITRVVKVVGHVASADG
                     FAEQPQVLNGASDLLVAIFGDAGRHARVATGAAELPRGAAVEIEVILAVS"
     gene            complement(23201..>24184)
                     /locus_tag="VFH05_14205"
     CDS             complement(23201..>24184)
                     /locus_tag="VFH05_14205"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679424.1"
                     /translation="QEIKILEDLLACRPVGTGIRPVLREGLWAVVLAVEHELTAEVRL
                     DRAYSTAAIAALEAALGHKVVGGRVASGEEALKEFGARHLSDGAPIVWTDGRQTCHVA
                     AHDSVWSGEVLYQRVRESRKALRDAWGMRRLVAHPLIGAVGALRFGPGRKDYAGEPPG
                     QTMLDLLNRASQILIGVGKVGDLFGGRGLTRSVVTSSWSQALDEVSGMFNKVPRGLIF
                     TGLDVIGSDAVNSAAAIEEFDRRLPAFLDQLRPGDLFIITGDHGRDPNKPHAVPTREW
                     VPLLITGPKLAQGVNFGLRPTASDLGQTVVEALKGESLTVGESFLDVLRPG"
BASE COUNT         5149 a         7332 c         6930 g         4773 t
ORIGIN      
        1 cgcgcacctc ctggtcaggc ggttgccgga attcgagaag gccaaacgat ttgccgtcac
       61 gctcgaaccc cgcatcggca gtcctcaccc caacggcccc atctctctcg tgggtgagcc
      121 ctcctaagtt cttttctcac ccgcccgccc tcgcgatcgt tttcacccgc caacccaccg
      181 attgccaagc aatcgttaac ccgggacgcg ctccttttct cacccgccca ccctcgcgcg
      241 ccgagacgcg ctccttttct cacccgcccg ccctcggcga ttctttccac ccacccgacc
      301 caccgattgc tatgcaatcg attacccggg acgcgctcgt cccagccggc ttgaccccgc
      361 cgggaaagct cgctacaacc ggcgcgatga tcgaccctgc gacgacctcc gattctcgat
      421 tcatgcgagc agcgcttgag caggcggcct gcgctgcggc cctaggtgaa gttccgatcg
      481 gcgctctcct cgtgcatgac ggcgcgatcg tggctcaagc ccataatttc cgggaacgct
      541 ggcaggatcc cacggcccat gccgagatga ttgtgatccg agaggctgcc aaggcgctcg
      601 ggcggtggcg attgatcgat acgactctct atgtgactct cgaaccctgc gccatgtgtc
      661 tcggcgccgt gattctcgcg cggattcctc gcctcgtgtt cgcggccaag gatcctaaag
      721 cgggcgcctg cggatcggta ctagattttg ccagtcaccc gcggttgaac catcgggtta
      781 ccgtgctggg agggattctc gaaggggaaa gtcaacggat gctggctgaa tttttcaagg
      841 ccatcagggc gcaggacgtg gacggcggaa ctctgtaacc gatttcaagg ggtctctccg
      901 tccataatgc gaacggtcca gtcgctcccg tcggcagaca cggccccctg ccatccagca
      961 ccgcaattga gccattgaat cgtccacccg ccctgcctgg ctgatgaatc caaacacctg
     1021 accgtcgcag atggccgcgg accgcaaccg tcaggaagaa tctcgcatcg ctgaagtgac
     1081 tctatgccaa ttccctgccc cttcagcacc gcttcttttg ccacccagta gcgatagaac
     1141 tgccacgttc gatccggttc ggttcgattt ttcagatcct catattcagc ggcggcaaaa
     1201 aaccgttcgc ccagtttgag ggcatcgagt gtttcacgaa cctgctcgag gtcaatgcct
     1261 acctcgcgtc ccttcgccac cgcaaccagc atgcgtccat gataatgcga taagttgaat
     1321 cgcagcgaat gtgaagaacc ggacgcattc agcaaaatgg gtttgccggt cgagccgaca
     1381 tcaaactcaa tggtttccgg agccactgcc agataacggg ccagcacccc gcgaagggcg
     1441 ccatgcgcca agataaagtc gttcccctgc gccgcatgaa tgaagcgttg cgccctggcc
     1501 cgctcttgtt cgctgagcca ggcttggcac cgttggagta ccgacgtttt gtgtacgagg
     1561 gcaaagctcc acacatcaac ggtttgatca tcgagcacaa cagtggcgac ctgcgtcgca
     1621 ggtgttggcg ggtcaacggg cgggaaggcc aacaccgttt gtagcgagcg ggaaatcatc
     1681 gttctcttgg atgggttgag atgcctatcg taccgactcg tgcaccgaaa gaaaaccaat
     1741 tgaaaatttc atggccgcgg agtaagctcg cccgatcgac tccctgaagt cattactcac
     1801 catcccgccg ggcgttcagg ggacaatcac ggagaggtgc gagagcggcc gaatcgggca
     1861 gtctcgaaaa ctgctgtcgg ggtaactcga ccgtgggttc gaatcccacc ctctccgcca
     1921 tagcaaccgc ctctaccaga ccaaaggggt accacagaga gtgacatcgc cctttgacta
     1981 acagagagag ggtgggattc gaacaggggt tttgaagggg atgtctcccc ttcatcggga
     2041 gcgtacgagg gggcgtggcc ccctctgtag gagagttgct gcaacgcgac ttcgaatgcc
     2101 caccctctcc gccaaaccaa gctcgctcgt gccggcggct cctcaggcgc agcctctctg
     2161 cattgacgcc gccggataaa cacctccagt gtcgttgctg caaaccgagc tcgctcgacc
     2221 cggaggcgtt aaattgctcc tggcgacggg atgaaaccca acagtatcgg gattcgaaca
     2281 ggggttttga aggggatgtg tcccctgcac ctttttctgc ttagctcgtg acctcgctgc
     2341 tggcctgcga ggacaatgtc gcagcttttt tcatcaacac ccgccgcagc tcgaccatcg
     2401 ccatcgtatc cctcgcacaa tactgcaaca gcgcctctct gatcttcagc tgttcaatcc
     2461 agtcttcttc gacaaagacc atccgatagt actcactggc cgcctgcccg ccgtcctgga
     2521 tcaggagatc tcgatagctc atcccgggca caatcgccgg caatacgaac ttgatcgagt
     2581 aggagccctg aaaccccgga tgataataat gctccttgat gacgggaagc agatcccaca
     2641 accgcctcgt cattgcggac aacgcctgcc gaaattcggg aaaggcttcg gccacttgtc
     2701 tcatcatcgc ttcttcatag gaggagtaca ccacgattgt gcccgcatcg ccgagcgatt
     2761 caattaacgt ctccgcccat cgtttacgtg gctccgttgc ctctccatga aggaactcct
     2821 gatgaatgat ctcaccggat tcgagctcaa tgtgattgga ccattgcacc ggtatcgctt
     2881 ggtagggcct catggcagga aaccgcggaa cggccaacat gacggtttca aagtcgagat
     2941 gatgaacggg atagacaacg gatctcagca cgctggcaag ccctgttgag atccattcca
     3001 cattgtcttt cacccggcgc tgagtgatgg acaagttcac gtcgtcgggg atgtcgtcaa
     3061 tcgtcgtaat accgttttgg accaagcctt ccaccatctt tttcacaccg ggcaagcgat
     3121 agatccaccg aggagatttg tcttgtgtgc agtgagccca aaattggcat tcatagggac
     3181 tatggcaacg ggcgtccggc tcgatcgccg gcgggtgtgg ttcggacacc acagccttca
     3241 tcgcgaggag ttgctcgggt acctttcctt gccggtcgac gacggccgcc gaaacctcgt
     3301 cgatggcaaa gagagcctgt aagtcgattt cacctccttg gtacagataa cccgtgttga
     3361 tatgcatgag gcaggtctcg gccacattga ggcccatccc cagcagcacg tgccgttgga
     3421 tcgcgagatc atcgaggtgc acatccttca ctcgggtggc cgatttcact tcgatcagcc
     3481 gccaagccgg agatgttccg tccgcgacgg ggatccgctc gagaatatcc acacgcacga
     3541 caaccccttc atgctcgaac gcgccctcga agatggcggg tattcgagag tcttcgagca
     3601 gcttcgcggt ttgaaagatc gccgcctccc gttggcgagg cacaggccag accacaactc
     3661 cccccgggaa gcgtcgccgt gccaacacac caatctccgt tcccatggcg aggatggctt
     3721 gcgtcgaagg gtcgggaggc gtggccagct caggccgcct gatttcgagg tacagtcgct
     3781 tgtgacattg caagcctgag aggaattttg atttggacag ccggggcgcg attggaggca
     3841 accggtccgg ctctgggaat gtcgtcatgt cggcaaaccc taattgatgc tcgccgtgtt
     3901 tgtccaggcc gctcagtacc gcttctgtta ggatgggttg atggccaacg gtgtcactca
     3961 aatccgccgc tccgtcatgg cactggccct gcccgttacg gtcagcagtc tgctccaacg
     4021 cacggaaggg attgtcgccg tgtttctagt cggcgggttg ggcgcggtgc cgattgccgc
     4081 ggtgggtctc ggccagctct tggctttcat cgccagcacc ctggtctccg gcctctcggt
     4141 cgggaccaac gtcatcgtcg cgcaattgtg gggtgcccgc cgccgtcatg acgcaggtca
     4201 ggccgcacgg catttcctcg ccctctccat cgccgtgtcg ctcgtcttga tggccggcgg
     4261 ggtctcgctg aaccacttcg ccatgcggct cctgggagct gctccggagg tcatcgcgct
     4321 gtcactgccc tattccacgc tcatctttct cgtcattccg ttcaccgtgt tactgcaagt
     4381 gctgtcgtca atcctccaag gcaccgggaa cacgaaaacg ccgatgtacg ccatgatcgt
     4441 cgtgaacgtc ctgcacatcc tcattgcctt tccgttgatt tatggccatt ggggattccc
     4501 ggcgctcggc gttcaaggcg cggctatcgc cgtgggcatc gcggagagcg ccggcacctt
     4561 gtacttgggc cttcgctgtc ttccgctgct gcaacgctcg cagcgcgtcc gatgggatct
     4621 ctttcgaacc atgtggcagg tcggcgcgcc ggtttccggc gaacgcatcg tccagcaggc
     4681 cgggattctc gcctacacga aaatcgtgtt gctttacggc acggtgtcct atgccgctca
     4741 tcaagtcggc ctgtcaatcg aatccttgtc gttccttccc gggtatgggt tcgccatcgc
     4801 cgccgcaaca atggtaggcc aaagcatcgg tgcgagaaaa tatacgaggg cgaagctcga
     4861 aaactgggag gccaaccggc tggcgatcgg catcatgtcg gcaatgggct tggtgttttt
     4921 ctttttcccc tacgcgctcc ttcgcgcgtt cacgagcgat gaatcagtga tcgagttggg
     4981 caccctcttt ttgaagatcg tcgccctcat ccaaattccg ctggcgctga ccatggtgct
     5041 ggccggctca ctgcgcggcg cgggggacac gcatttcatc atggtcgcaa ccaccatcgg
     5101 catgtgggcg gtgcgggttc ccattgcctt gatcacagga gtctggctcc acttgggcat
     5161 cttctatgtg tgggtggcca tgattgcaga ctggacgctg cgaatgggct tgatgttgtg
     5221 gcgttataga tcggagcggt ggaaggccat tcaggtgatt cgatagtcag gagcgcttgc
     5281 tttctgtaca gacggacggg cgttcggacg gccgggtgtc tttcccggat ccgatgcgca
     5341 cttcgatgcg gcgcaccgct ttgacattgg cacagaggtc tcccatgcct ggagtgatca
     5401 ggcggtacgg cccgcccttt gtctcgggca gcggctgatc atccacttca tacagcaaca
     5461 gcccaaagtc tctagcctgc tggagcgtca gggtcgcggc gtagttccca tcattcgaat
     5521 gaaaggtcac gtgatcggcg gcaatggcca gcgcaggaat ttcgagcaag cctttcacgg
     5581 taatggcccg tcctctcatg ctgggcatga gcgtattgac gtcactgacc tggcactcgg
     5641 caggcagctg acgcagcgcc atccggtcaa agagcaccgg ctgaacgaca gcgccgtcga
     5701 tctcgacgat gccgggaccg gtccgcggct tctcagtcac ggtcttgatc atcgcggtca
     5761 acgcttcgtt gaccggtgtc ggaatgccca attcacggcc ccgctctacg atgtagccgt
     5821 ttaaattcct gatttcggtc tgccgcccgg ctttccaatc gtcgtacatc gaggtgtgga
     5881 tgtcccgaat ctcttgtgac catctcacca cccgatccga catatccgaa ggcaacggca
     5941 ccttgagcgc tgcagacacg gccgtaattt cttgaacaat ctgtctgatg acgtgcatca
     6001 tctccggatg gtccagcgct gcggacacct tgtcgtcaac cagaaccgtc aggggattga
     6061 acacacagtt ccagcacatc ttttcccatt tcgcgcgccg gatatcttta gacaattggc
     6121 agggaagacc ggcagaggca aacagatctc gaatctcaag gaggcgcgcg ctttcgtgcc
     6181 ccatcagttc accgatggcg acgttcccct tcttgtaatg gtcgatgacc ccgggagcga
     6241 cgatcttcga gtatatgtag gcgaccccgc cgacgacaca atcccgcttt acccgttcaa
     6301 tgatccggtc ttcggtatca attccgtttt gcaacgtcaa aatgacggtg cgctcggtaa
     6361 gcaccggctc gatctgtgtc agcacctcgt cgaggtcgta ggctttgacg gccaggatga
     6421 tcaactccgg ttggggaagc tgccgcgcgt ctgttgcggc gcgcggatga acggtgacgg
     6481 tgccggacgc gctgcggact tgaagtccgt tcttctgaac ggcagccagc gtcttcggcc
     6541 gcagcaaaaa agagacgccg ggatgatgct gggccaaatg cgcgccgaaa aagccgccga
     6601 ctgaaccggc tcccaccatc agaatgttat tcatcgagaa actccaaggc tcaatacgtt
     6661 gcggaaattg gtgcgcctac tatagcatgc ggcctccacc ggacacaggt gatgaggcga
     6721 tgaccagcgg cagtgatctg gatctgctca aacgcaaact ccagtccgcg cgatccctgg
     6781 cgatcttgac cggcgccggc atttcagcgg atagcggcgt cccgacgttt cggggccccg
     6841 acggcctgtg gcgcaacttc cgcgcggaag accttgccac ccctgaagcc ttcgcgcgag
     6901 atccgcggct cgtgtgggaa tggtccaatt ggcgccgcga gttgatcgca accaaaagcc
     6961 cgaatccggc ccacgtcgcc gttgcggaat ttgagcggcg cttcgaggaa ttttggctga
     7021 ttacgcaaaa tgtcgacggg cttcatcgcg cggcgggctc ggaaaaactg tccgagattc
     7081 atggcaacat ttggaaggtc cgctgtacgg cctgtggaac cgtagaagac aaccatgacg
     7141 tccctattgc aatcctgcca acgtgccgtc attgccaagg tctccttcgc ccccatatcg
     7201 tgtggttcgg ggaatcgtta tgcccggacg acttggcttg ctgcgcagca gcccttcgag
     7261 cctgcgaggt gctgctggtg atcggcacgt ccggtgtcgt ttatccggcg gccggattca
     7321 cctccgtcgc aaaggacacc ggcgcattcg tggccgagat caatttcgag tcgacgccac
     7381 aatccggcac aatggatctt accctgaaag gacgggcgaa ggatctggtg cccttgctgc
     7441 tctaaacggg ccgagacttt cttcctgccc ttcacttttc tagtgggggg cgatcagcgg
     7501 gagcaggtca tcgagcgcgc gtatggctgc aatccctggt ggtggcggct gctttccttc
     7561 acgcgcgagc agcaccgccc gaataccggc cttcctggcg ccttccacat cgtctgtgag
     7621 gctgtcgccg acgtgcagcg catccgccgc ttcgacggca tgtttttcca aggccagctc
     7681 aaaaattttg ggggacggct tggccgcatg ggccaagctg gaaatagtca ccgtgtccat
     7741 atactcggcg atcccgagcc ctctcagaac gttgaacaaa cgcgaatcaa agtttgaaac
     7801 aatccccagc tccaggccac gactccggag ttccttcagc gttggcacga cttcgggata
     7861 gagcttccat gaagagggat cggcaaagac gtcgaatacc cgctcaaaaa attcatcgaa
     7921 ccgctcgaac atcccgacac gatagaacac gttgtgcacg acatcgaacc accagagccg
     7981 ctcgctctgt ttaatctgcg ccgattcggt cgctgcaaac accggaggcg gcgcatcgcg
     8041 aaacgcccgc ctgaacgcct ccgtgatcga ctgcagggag tctgcctttc gtgggtagcc
     8101 gaactccgcc gcataccgga gatagatttc ggcgacggat ccgttcacat gaaacagcgt
     8161 ctcggccgca tcgaacaaga ccacctgaat cgactggctc atctgtggat gtcccagggt
     8221 gcgtgaagac tcgctgctac ggagaatacc tcaaatggca tggcgcatct attggccgtt
     8281 ttcttgacaa agaatacccc ccctgctagc ttcgaactag ctcaataaaa ttggagactt
     8341 aagtgccttc aacgatcttc gtcatcgaca gcagtccggc tgtccgccgt atggtggaac
     8401 agatctcgac ccccgaaggg tttgacgtgg tggggtttca ggacggcccg gcggcgctcg
     8461 aagccgcccg caggatgagt cccgcgctga tcatcaccga ctaccacctc gacagcatga
     8521 ccttttcggg gttctgcaaa gaagtgcaca aattggacaa tctcgctgat acctatatca
     8581 tcgcgctcac gggtgcgacg gacaagctgg aaaacacgca cctgaaggcg cttggcgtca
     8641 aagccttcct caagaaaccg tttcagtcgg aacatttgtt ggacctcatc aaagacctgg
     8701 accgtacgaa cacgacgaac atcagcggaa agaagaaacg gtcctggccc ccgacatcga
     8761 gcgcgcttga ttccgatgat gagtgtgcag gcgattcggc cgacgccatg agcgacgagg
     8821 agttgcatcc gatcattccg atgaccctcg acgtgacacc cgttaagaaa gaggccatga
     8881 aaccgcccgt gcccgaatcc gcctcgactc cccctgcagc ggaaccggaa gaagcaatga
     8941 aaggcctctt cggccagttg ttggaatcga tgtccggaaa aacagagaag aaaattgccg
     9001 acatgctgcc ccacatggtg aacacagggc ttgaagctca ggtgtccaga gccgtcgagg
     9061 cagaagtgcg tctgcaactg ggcgccgccg tgtcgcagga gagactgacc gagataatcg
     9121 aaccgctggt catgcaggcg ctcccgaaaa tcttgacgag ggaaatggcc gtactggaac
     9181 cgatcatccg acacagcatg gtcgagatcg ccagcccgtt gattaaggag cacatcgagc
     9241 acatggtgcg ggaacaggcc gagacggtca ggcaatcact tcaggatgcc gtacgcgaac
     9301 acttgagatc ggtggaagag gaggttcggg cgcaactcaa agaggctgcg acggctgaaa
     9361 cgaagaagct tgccactgac gtggtccgga ccatcgcaga agatcagatc agaacaagcg
     9421 tccagatggt agccccctca atcgtcgaag aacagattcg tgccgaaatc gctcggctga
     9481 ccgaggccgc ctgacagcga aatcttctag ccttttttcc cctttttcgt gccgcgtcgc
     9541 cctttcgcca acgttttcat ccgttggacg ttcttccgat gcttctttct ggtcttgcgg
     9601 tccttctctc gtcccgtggc cataatctct cctcggtcaa gcgtgaatga gcccgtcgac
     9661 tgcccggcaa ttcggcgatg atatcacaca agcctccctg cctctcaagg agaagaagta
     9721 accttgaggg gtcccggcgc gcatgttaag atgcctcgcc ttctctccag cgcaaaccat
     9781 ggcccctctc caactcgaca aaacgtacga tccgaaagcc gtcgaatcgc gatggtatca
     9841 gttctgggct gaacgccaat actttcacgc cgatccctcc catcctggcc ggccctacag
     9901 catcgtcatt ccgcctccga acgtcacagg ctcgttgcat gtcggccacg ccttgaatca
     9961 ttcgctgcaa gatatcctga tacgttggcg ccgcatgcag ggtcgcaaca cactctggct
    10021 tcccgggacc gaccatgccg gcatcgcgac gcaaaacgtc gtcgaaaagc agttgctggg
    10081 cgaagggatc tcccgtgaag cgctggggcg ggatggtttc gtcgagcggg tctggcagtg
    10141 gaaggcggcg tcggggaacc gcatcatcga gcagcaaaag cagctcggag aatcctgtga
    10201 ctgggaacga ctgcgcttca cgatggacgc gggattgtcc aaggccgtgc tggaagtgtt
    10261 cgtccggctc tacgaagacg gccttatcta tcgtggcgaa cgactaatta actggtgtcc
    10321 ccgctgcctg accgccttat ccgacattga agtggaacac gaggacgtga agggcacgct
    10381 ctatcacatt cgctatccgt tgaccgacga cccaagcacc tcgttgaccg tcgccaccac
    10441 acgaccggaa accatgctcg gcgacacggc ggtggccgtg catcccgacg acccgcgcta
    10501 tcgccatctc ataggcaagt cgatccgatt gccgctcact caacggacca ttcccgtcgt
    10561 gggcgatgcc atcctggtcg atctcgaatt cggcacgggt gccgtcaaga tcacgccggc
    10621 ccatgatttt aacgattatg aagccggtga gcggcatcac ctcccccggc tgccgatatt
    10681 caaccatcaa gcgttgctcg atccggcgtt gatgaagaca gccggcgtcg acgcggatgt
    10741 catctccgcg gtggcctccc ttcccgtgtc taaagcgaga cccaagattg aacaggcctt
    10801 gaaagatcga gccctgctca gcaaggttga agatcacagg atggcgctcg ggaaatgcta
    10861 ccggtgcaag acggtcgtcg agccctatct ctctcctcaa tggttcgtcg agatcaagcc
    10921 gctggcggaa ccggcgatcc gagccgtcga ggagggccgc atccgcatca ttcccgaggg
    10981 atgggtaaac aattacctcg gctggatgcg cgacatcaaa gactggtgta tctctcgcca
    11041 aatatggtgg ggccaccaga ttcccgcctg gtactgcgag cgttgtaacg agccgttcat
    11101 ccataggcag acgtccacca accaggccgg cgacgtcgtt acacgcatca ccctccttca
    11161 aggcgccaaa ccggtcgtcg cacgatccac gccggccaag tgtccgcagt gcggcgggga
    11221 gcagtggctg cgtgatcctg acgtgctgga cacctggttc tcctccggac tctggccgtt
    11281 ttccaccctg ggatggcccg agcagactcc tgagctcaag acctactatc cgacggcgac
    11341 gctcgtgacc ggactcgaca ttttgttctt ttgggtcgcc cgcatgatca tgctcggcct
    11401 ccgctttatg aacgacgtcc cattccgcga cgtctacatc catgcgctcg tgcgggacgc
    11461 tgagggccag aagatgagca agtcaaaagg caacgtcatt gatccgttgc atgtgatgga
    11521 ccagttcggc accgatgccc tgcggttcac actggcctcg atggcgtcgc ccggccgcga
    11581 tcttaaattg gcggaagaac gcattgaagg ctatcgtaac tttgccaaca aaatctggaa
    11641 tgcagcccgc ttcggattga tgtatctgga cggagcacga acggcagtac gcccgtcaga
    11701 acggccattt cccgatcgat ggatcttgag ccgccttaac cacacgattc agaccgtcac
    11761 gtcagagctc gaggcctatc gcttcgaccg cgcggcgagc gcgctctatc aattcatctg
    11821 gcatgagttc tgcgactggt acgtggaatt gatcaaaccg gtcttgcaga atccctccca
    11881 tgcggacggc ccggtgacgc ggcagaccct cgtggagacg ctcgaagtca ccatgcggct
    11941 cttgcatccc tttatgccat tcatcacgga agaaatctgg caaaccattc cacacgaagg
    12001 cgagagcatc gtcattcgac cttatcccgc atcagagcag acgtgggacg atcccgtaac
    12061 agaggagcaa tttctcctgc tcgagcaaac ggtcggcttg gtccggaccg ggcgggtgtt
    12121 gctcaactat ccgccagggc agccgatcga gttttcaatg acgcatgacg atcccgccaa
    12181 gctgcggcaa ctcgaacacc ttcaacccca tgtgtcccat ctcagccgcg gaaccgccga
    12241 catgctctct aatggggtga gtccggtagc acggcgcctt cgtctcgtgg ccggagggct
    12301 ttcgatcgca ttggtcatag ccggtgacgt cgatctcaag aaggcgttgg accgcatcgt
    12361 gaagcaacgg gaacagcagg aaaaggagct gacacgcctc gaggggaaac tgaataacca
    12421 tgagttccgg ggcaaagctc cggcggacgt cctcgcagaa cacgaaagcc ggctgcgaag
    12481 cctgcagaat gatcaagcgc tgcttgccag cagcgagcga caactgcgcg ccctgctcgg
    12541 gccgtgacca tgccggagct gccagctgcc gacattcgac gcgccgtccg gctggctctc
    12601 gaagaagacc tcggccaagg cgacgtgacc acatcggccc tttttccatc ggcggttccc
    12661 gcccgggcac gcatcgtcgc tcaacagccg ctcgtggtgg ccggcatggc agccgccgtc
    12721 cagacattcc tggcagtgga tgcctcgctc aggctcgccg tgctcaagaa ggacggcgcg
    12781 caggcgttga accgcgagac actcctgcgc attgaaggag acggccgatc aatcttgatg
    12841 gctgaacggg tcgcgctgaa cttcttgcag cagctgtccg gcattgccac gctcacacaa
    12901 cgattttgcc aggctgtgga tggccgcgtc gccattctcg acacgaggaa aacgattccg
    12961 gggtggcggt cgattcaaaa atgggccgtc gcccttggcg gaggaacgaa tcaccggcgc
    13021 tcgctcgctg atgggattct catcaaagac aatcatctca aactattgag acacaaacag
    13081 ccggtttttc aatcctgcac actggcacgc caaaggggtc cggccggtat gccggtgatc
    13141 gtggaagcgg aatctctcaa ggaagtcgct caggccgtcg cggggcaggc ggacattatt
    13201 ctcctcgaca acatgacgcc cgccatggtc aagaaggcca tcaaaatcat cgacgggcgc
    13261 gcgctcgtcg aagtctcggg aggcattacc ctagacaacg tgcgcgccat ggctgcggcg
    13321 aagcccgacc ggatttcgat cggcgcactg actcactcgg ctcctgctgc aaccatcacg
    13381 cttgaactgg aacgtgcagt ccaaggccgc caacagagac gctagccggt gaaacacacc
    13441 tcgcccttga ctgtcgacga tattgaaagg atgctcgcga cccgatccct gggccgccgt
    13501 atcgaattgc gcgatcgtat tgactcgacg aaccgggaag cgatggcgct aggccaagcc
    13561 gacgccgagc atggcacgct ggtgctcgcg gactcgcaaa cggcaggacg gggccggctc
    13621 ggacgagaat ggttttctcc accagggatc aacctctatg cctccatcgt gatccggacc
    13681 acgctcgata cccaacgtct gtctcagtgg ctctcttggc tgccgctgat gaccgcgttg
    13741 gggaccgccg aggccattga atccgccgga gccgccaggg tcagcctcaa atggccgaac
    13801 gatctcctca taggcgatcg aaaagtcgga ggcattctgt gtgagagcgg tgcttcggta
    13861 cgttcgggac catttcaagt gatcggcatc ggcatcaatg tcaatggaga acgagcgagt
    13921 tttcctcgcg ggctccgaga ctttgcaaca tccattcgac aggagacagg acgggagatc
    13981 gaccgccacc ggctcctcgt ccggttgctg gaggaactgg agctatgtct cgaggagttc
    14041 ctgacgcgtg gaatcgaccg gctcgcatgg gagtaccgtc gccgatgtac gacactcggg
    14101 aaaacggtca aggcggtgct ggcggatggc aacgaatacg tcggcacagc cgagggcatc
    14161 ggtgatgacg gttcgctgac aatggttcac gaggcggcgc gacctggagg ccagtccgaa
    14221 gtccggcagc tgcgtgccgc ggatattacg cacctccgat gaagctttcg gtagcgaaac
    14281 cacggccaag cccgtaaaat acaccgcatg ctgttggtcg ccgacatcgg gaacacgaac
    14341 gtggtgctgg gcatctatga cggccaggtg ctgaaggcgc attggcgact ggccacagat
    14401 gcgaaaacca ccgccgatga gtacggcatt ctgttcacca acctgctggc ttctgccggc
    14461 ttccttcccc ggcaggtgtc aggcgcgatc atgtccagcg tcgtgccgac gctgaccgcc
    14521 acgttcgaaa cgctgattga gcaatacttt catcaacggc cgctgatcgt cacgtcagag
    14581 accgatacgg ggttgacgat ccggtacccg aaccccaaag agatcggcag cgaccggctc
    14641 gtcaatgccg ccgccgccta tcacaagtat ccacgggacc tgattatcgt ggacttcggc
    14701 acggccacga cattctgtgc cgtgacaaaa gaagccgaat atttgggcgg agtcatcgcg
    14761 ccagggctgg gcatttcggc cgaagcgctc ttcacccggg cagccaagct gtcgaaagtc
    14821 gaattggcca ggccgaaaac gatcatcggc acggataccg gcagcagcat ccaatccggc
    14881 ttgctctttg gctacgccgg cctggtcgat acgctggtca aacgcatcga acacgagctc
    14941 ggccgctcga cctccgtaat tgccaccggc gggttgagta ccattattgc ctccgaggcg
    15001 acctccattc agatcatcga acccttgctc actcttgacg ggctggcact gctctatcgt
    15061 cgcgccaatg ggtccgctcc gactgcctgg gtttgagtct ctgtggctca caagtaatta
    15121 atttatctcg gtgctgttga cttattagtc gtggtgacta tctcgcctgt gtaacaggac
    15181 tgatcgtgac ggacgaatca ttaaacacca atacattcga caacttagac tctgactctg
    15241 gtggacccgc ttcttccggt gactcgctgg ctgaatcggc ggcgagggac ggcgagctga
    15301 agacgctgaa cgacaagtac ctccggcttg ccgccgagtt cgacaattat aagcggcttg
    15361 ctcagcgcga tcagcgggac caaatccggt tcggcaacga gcaacttctg aaggaactcc
    15421 tgccagtcgt cgataatctt gaacgggcca tcaaggcggc gaaggacagt aagaccgcac
    15481 agggactggt tcagggcgtc gatctcacgc tcaagcagct ggaaagtgtg ctgagcaagt
    15541 tcggagtcca gtccatcccg accatggggc aacgctttga cccgtccggt catcaagccg
    15601 tcgcttccgt gccttcaacg caggtgcccg atcagcatgt ggtggaagaa tttcagcgtg
    15661 gataccggct gcatgaccgg atcctcagac ccgccatggt gaccgtgtca tcaggcgcag
    15721 gatctggatc agatggggat gaaatagaca actcaaattg aacaggagaa tttcaggctg
    15781 ttcaaaaagg tcgtccggcg cgctggcgga ctttttcaac agcctgagat gaggaaggag
    15841 cacatcatgg gtaaagtcat cggcatcgat ttagggacca ccaactcatg cgtcgccgtc
    15901 atgagcggcg gcgatccggt tgtaattgcg aatgcggagg gggcccgcac gacgccatcc
    15961 gtcgtggcga tcaccgacaa aggcgaacgg ctcgttggtc aaatcgccaa acgccaggcc
    16021 atcaccaatc ccgaaaacac cattttctcc gtcaaacgcc tgatgggacg caagttccgc
    16081 agcaaggaag tgcaggaggc catgaagcgg ctgccgtaca aggtcgtcga agccgacaac
    16141 ggcgatgccc atgtcgagct gcgcggcaaa cgctacagtc cgccggaaat ctccgccatg
    16201 atcctgcaga aaatgaggca gaccgcggag gattacctgg gcgagaaagt cacggaagct
    16261 gtcgtgactg tcccggcata tttcgacgac agccagcggc aagccaccaa ggacgccggt
    16321 cagatcgccg gactgaacgt cttgcgcatc atcaacgagc cgactgcggc gtcgttggcc
    16381 tatggtctcg ataaaaagaa agacgagcgg atttccgtct atgacctggg cggtggaacg
    16441 ttcgacgtct cggttctgga aatcggcgaa ggggtgtttg aagtcaaatc gacgaacggt
    16501 gatacctacc ttggcggcga cgatttcgac ctgcgcgtca tggattggct cgtcgacgag
    16561 ttcaggaaag atcaaggaat cgatctgcgc aaggatcgca tggcgctgca gcggctgaaa
    16621 gaagcagccg aacgtgccaa gatcgagctg tcatcctcac aagaaacgga aatcaatctg
    16681 ccgtttatta ccgccgatgc gagcggtccc aaacacatgg tgatcaagct gacccgcgcg
    16741 cggctggaac aactggtgga cgacttgatc cagcggaccg tggaaccctg ccggaaagcc
    16801 ctgactgacg ccagcgtgtc ggcccgcgac atcaacgaag tcgtgctcgt cggcgggatg
    16861 acccgcatgc cgaaagtcat ttcagtggtg aaagactttt tcgggaaaga gccgcatcgc
    16921 ggcgtgaatc cagacgaagt ggtggccatc ggggcagcca tccagggcgg agttctgaaa
    16981 ggcgacgtca aggacgtgct tctgctcgac gtcacgccgt tgtcactcgg catcgaaacg
    17041 ttgggcggcg tctttaccaa gctcattgag cgcaatacga ccgttcccac gaagaagagc
    17101 caggtgttct cgacggcggc tgacaaccag acagcggtga ccatccgcgt gttccaaggg
    17161 gaacgtgaga tggccaacga caataagttg ctcggtcaat tcgatctcgt cggcattcct
    17221 tccgccccgc gcggcatgcc tcagatcgaa gtgacgttcg atatcgacgc gaacggcatt
    17281 gtgcacgtgt cggccaagga tctggccaca caaaaggaac agtccatcaa gatcaccgca
    17341 tccagcggat tgagcaagga cgaagtggaa aaactcgtca gggatgcgca gacgcatacc
    17401 gaggaggaca agaaacgccg gaaagcggca gaagcgaaaa accaggcgga caacctgctc
    17461 tatcaaacgg agaagaatct caaggagcac ggcgacaaga tctccgccga tgataggacc
    17521 aagatcgagg aagcggtcac tgcgctgaag aaagcgatgg aaggcaccga tcccgacgcg
    17581 atcgagtcgg ccacgcaggc gttgacgacc gcctcgcata aactggccga ggagatgtat
    17641 aagaaggcgt cggcctccgc agcgaccggt gccgggacgg ataccggcgg cggagccgaa
    17701 agtccgaacg gtgcaaagac cgatgaaaaa gtcgtggacg cagagtttga agaagtagac
    17761 aaagataaaa agtaagctag aatacggcta ctcagggacc gagtttcgtc gactacggaa
    17821 ctcggtcttt ctgcgcaacc accgaaaggg acaatggctt ccgtgtctaa gcgagatttc
    17881 tacgaaaccc tgggcgtcga acggacggcg tcagaggaag atctcaagaa agcctaccgg
    17941 aaactggccc gccaatttca tcccgacctg cagaccggcg agcagcagaa gaagcatgcc
    18001 gaggaaaaat tcaaagaaat caacgaagcg tatgaacacc tgagtgatca ggaaaagcgc
    18061 aaacgctacg atatgttcgg ccatgcgggc acccaggcgg gctcgggctt tgaaggcttt
    18121 gatttcggcc ggggcggctt cggggatgtc ttcaacgaca tctttgagga ctttttcggg
    18181 ggccagcgcg gcggcaaccg tgcagagcgc ggcaacgatc ttcaatacaa tctcgaaatc
    18241 accttcgaag agtcagtcta cggcaaagaa gccaaactca agatcccccg ctgggagaca
    18301 tgcagcgaat gcaaaggcag cggtgccaaa tcggcctcgt cggtgaagac ctgtcccagt
    18361 tgtaagggcg ccgggcagat ccggcttcaa cagggtttct tcagcgtcag ccggccttgc
    18421 ggccaatgcg aaggttccgg acatatcatc acggaaccct gcacggcctg ccagggccgc
    18481 cagcggacat accgcgagcg cacgatctcc gtccacattc cggccggcat tgaaaccggc
    18541 atgcgcttgc gcctatcgaa cgaaggcgaa catggcatca acggagggcc ggcgggcgat
    18601 ctctacgtcg ccattaccgt caaagcacat cctattttcc agcgcaaagg caacgacatc
    18661 gtctgtgacg tgccgatcaa ctttgtcacc gccgcgctcg gaggaaagat tgaagtgccg
    18721 acgctcaagg gcacgaccgt gatgaaggtg cccccaggca cgcagccgga taaggtgtta
    18781 cgactgaagg gactcggcat tcccagtctt aagaaccaga ccaccggcga tcaattgttc
    18841 acgatcaagg tccatattcc taccaaactg actgccaagc agcgggaact gctgaccgaa
    18901 ttcgccaaag agagcgggat gtccatggag gccgacggcg acggcttctt cgacaagatg
    18961 aagacctttt tcgagtaacg cgcgcaaccc actcgagatt cccttccgca tgccgacgtt
    19021 tttcgtggca gccgattcgg tcgtcccgcc gaccgtgcgt atcacgggcc ccttgctcca
    19081 tcatctccgg caaagcctcc gattacagcc gggtgaaacg ctcacggtga ccgacgatgg
    19141 tggccgacgc taccgcaccg aggtgggtga gatcacggcc ggtcacctca ctgcgcgcat
    19201 ccttgagacg acaaccgctc cgccgaaaac cagtccctcg atcgtcctgg ctcaagccct
    19261 cctcaaaggg gaaaagatgg attggatcat ccaaaaggct acggagttgg gtgtcgatcg
    19321 gatcgcgccg gtggtgacga ctcacggtgt cgtcaagcct cgtgccgaac gcctcgatca
    19381 tcaacgagcg agatggcagc gcattgccct cgaagccgca cagcagtctg agcggtggtc
    19441 ggtgccaaca atcgatgaac cggctacgct ctctcaggtc atgaccgcgt atgacgccag
    19501 cgccatcaaa ctcttcttag ccgaacgatc gagcggaact tcgttgacgg gcatggcagc
    19561 cggcctgggt gcagagcacg cgatcctcct cgtcgttgga ccggaaggcg gctgggacaa
    19621 agatgaattg cgcttcgccc aagaacaggg ctgtcaggcc atcaccatgg ggaaacgtat
    19681 ccttcgagcg gaaacggcgg cactcgccgc cattagcgtg ctgcagtcga ggttcggcga
    19741 attgggatag tcttacttgc ttaacttgag gtgcacgacg gtgccctttt ctccgctggc
    19801 ccagccgtgc agggtcgtag gaaaggacaa gccgaaaagc gagacattcg cgaccgctcc
    19861 ctgttcctcc caggtaaatc cgctgtccgc tgtatgatag gccatcccgc gttcgcccac
    19921 gatccagccg gttttggggt cggtgaaacg aatgcgaagc aagtccgtca gcctggtgca
    19981 ggtccgtgaa caagggaggg tccgatcgac ccagcggccc cctccgtcgg tcgtctgaaa
    20041 catggcgcct gcattgccga ccacccaccc ggtcattgga tcggaaaaat agacatcaaa
    20101 caacgtgacg gatccctggg tttcctgagc cagccaggtg gtcccgccat cttccgtggc
    20161 caggacggtc cccacggcgc ccacggcagt tccccgcttc ggcgtaagaa attgcacggc
    20221 atagagagcc gcggtggtgg gactggactg atccgtccag tgggctcctc cgtcggtcgt
    20281 atgaagaatc gttccgttgc ctccgacgac ccacccctcg tccggcgaga cgaaggacaa
    20341 cccatacatg ggcgccactg tctcgatggg ttgcgccctc caactggagc ctccgtccgt
    20401 tgtatggcgg atcgttccat tagcaccgat gatccatccc tgcttgtcgt cgacaaaaaa
    20461 tacgcccgtc agttgtaccg aagtgccgct ggtctgtttc ttccattttt gcccgccgtc
    20521 gacggtcttg acgaccgtcc ctcctgagcc gaccgcccat cccaggttgg catcgcggaa
    20581 atagacgctc atgagcgtca cttccgtccc cgttttctga ggcgcggctt tcagctgcga
    20641 ctcggcagct cttgtgggtg aaataatgaa tgtgattaaa agcagactga gggggacgaa
    20701 aagcaggatg gtcaaaaatc tcgtccagca aggccgcagc gagcgcagag gcgaagcgta
    20761 ctttgagttg tacgttgagc ctctaagcga tgcgagaaca cagctggcgg gattttggac
    20821 catcctgcta gtcgttggtg ctgccttcgg gcctggactg gaaaaagctc tggtcgggca
    20881 tccccttggc ttcgatcgtg aacgtgccgg cttcgagcgt cacccacttt ttcggggtac
    20941 agcaggtgcc atccggaagc agatcccagg agccccggcg gatgatcagg ggaccggtcg
    21001 agagatcagc attgaactgt ccaggcttgc cgatgccgaa caaatgacga tgcgggacgc
    21061 gcgctttaat ttccgtatcg gtccaggaca ccacaatggc gccgatgttg ttgaacagga
    21121 cctcggttcg cgagacgttt tcgccgagtt cgatctgatt gcgcttgaag gcaccctctc
    21181 caagtcccgg ctgtgtcgct tccgtcgtct tgataaacga gccgaatccg gaccctttga
    21241 tggtcaccac ttcgtccaac ccggctgagg caggcgtgaa ggactcgatg gtcggcacaa
    21301 caagggtgaa cgttccggcg gttgtcgata ccgtccctcg ttcgctgcag caggatccat
    21361 ccgctgccgg cttgggtgcc ccgcgggtga ccaccactgg accacttttg gcgctgaatg
    21421 gcacccagac gtcgatccga tcgtcgtgcc aacggtaaat caccgccggt gcaccgccga
    21481 tttccacgcg attttgaccg gtatcgaagt ccgtatagtt gtagggcgtc gccccgctct
    21541 ctgagtacgc gccaaagtgc tctccgtgga ttctgaccag cgtgccgatg ggggcgctcg
    21601 caggagagag ccccgtggct ttaggagtct ggacggtaaa ggtccccagc gtccggtctt
    21661 gtccgtgacg ggataacacc accggtcctg tttccgcatt gagaggcaca tgcaccacga
    21721 tcgcgcgatc gctccactgt gcaaccgggg cctcatgtcc gccgatcgta atgagatcgc
    21781 cggccgccct ttcttgacca aatccctgcc cgaacagcac gactttcgtg ccgacggggc
    21841 cggtcagcgg atcaacctgc acagtcggaa tcagtttcag cgaaacggcg ttgctgacgg
    21901 tatgttgaac cggggcgcag cacgatccgt cgggcaaggg atcggaagaa gccaagcgaa
    21961 cggcgatgtc tccggacgtc gcattggccg gcacgtccac ttcgatttta tcgtccttcc
    22021 agcgtcgtgg cctgaccact aagtttccca ccatcacgtc gttgacgccg aacatcgtgt
    22081 tgggatcacg gggtccggct gtgatgccaa aatgctcgcc cacgatttcg actgcatggc
    22141 cgggctcgat ggcgggagga tgaatggaaa gaattttcgg atagacgacc gaaaaggtac
    22201 cggccgcgag tttcttcttg ccgatcagca cctcaaccgg accggtctgt gctttgaacg
    22261 gcactttcac ttccacgacg tctgaatccc aacgttggat cagcgctggc actcctcgga
    22321 acaacacgcg gttcacttga gcagacttga agtgacccaa tcccttgccg acgatagaga
    22381 ccgtcgcgcc ggggacgccg gtcgcaggct gcagttcgag acgggcggag ggcgaagcat
    22441 ccgtcccctg atcactgccc aaggccacca gcaagacagt gccgaagaca atgatccaac
    22501 gactgagata ggtcttgacc atcatgtgtt gagagccctg cgtggcacga gaaccccacg
    22561 cacgagcctg gagggtaacc ggggtacagc gtgaagcgag ggacgacgga ctataacaag
    22621 cggttttttc gggtgtcaag ccgcagagcg cagggacacc ggcctctagg cccttcattt
    22681 tcttcaggaa acagccaaaa tgacttcgat ttccacagcg gcgccgcgcg ggagttcagc
    22741 ggcaccggtg gcgacgcgcg catgccgtcc tgcgtccccg aagatcgcaa cgagcagatc
    22801 ggatgcgccg tttaacacct gcggctgctc ggcaaatccg tcagccgagg cgacatgacc
    22861 gaccactttc acgacgcgcg tgatgcgatc gagacttccc agttcctgtt tcgcaatggc
    22921 aagagcattg aggatcgcga ccttggctgc ttcgacccct tccggaacgg tcaaatcacg
    22981 tccgagcttg cccgtgaatg ccaattgtcc gtttcgcatc ggcaggacgc ccgacaaaaa
    23041 taacaaattg ccggtccgaa cggcagggac ataggtcgcc acgggctttg gcgcctccgg
    23101 cagttctaat cccagggatg taagcttttc ttcatacgtc atcagaaaga gtccactcgg
    23161 ctcactgctc cggctgtccg gacgacaagc gtcatgttgg tcatcccggc ctcagcacgt
    23221 cgagaaaact ttctccgacg gtcaaggatt cccctttcag cgcttcgacg acggtttgac
    23281 cgaggtcaga ggctgtcggt ctcaggccga agttcacccc ctgggcgagt ttggggccgg
    23341 tgatgagaag cggtacccac tcgcgggtcg gaaccgcatg aggcttgttt ggatctcgtc
    23401 catggtcgcc ggtgatgata aacagatcgc ccgggcgcaa ttgatccaag aatgccggaa
    23461 gccgtcgatc gaactcctca atggccgcag cggagttcac cgcgtccgaa ccgatcacat
    23521 ccagtccggt aaagatcaac ccccggggca ctttattgaa catgccgctg acttcatcca
    23581 gggcctgact ccaggaggag gtgaccaccg acctggtcaa tcccctgccg ccgaacaaat
    23641 cacccacctt cccgactccg atcaagatct gactcgcccg attcaagaga tccagcatcg
    23701 tctggcccgg cggctcaccg gcgtaatcct tccggcccgg tccgaatcgc agcgccccga
    23761 cagctccaat gagcgggtgt gcgacgagcc gccgcatgcc ccaggcatct ctcagcgctt
    23821 ttctggactc tcgaacccgt tgatacaaca cctcaccgct ccacacgctg tcatgcgcgg
    23881 ccacgtgaca cgtttgccgg ccgtccgtcc agacaatcgg cgctccgtcc gagagatggc
    23941 gtgcgccgaa ctctttcaga gcctcctcac cggacgccac acgtccaccc acgaccttgt
    24001 gtcctagtgc cgcttccagg gctgcaatgg ccgctgtcga atacgcacga tcgagacgga
    24061 cctcggcggt caactcatgc tctactgcga gaacgactgc ccagaggccc tctctaagga
    24121 ctggtcggat acccgttccg actggccgac acgccaagag atcctcgagg attttgatct
    24181 cctg
//
LOCUS       DASSCQ010000225         9084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_666872, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000225 DASSCQ010000000
VERSION     DASSCQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666872"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..650)
                     /locus_tag="VFH05_14210"
     CDS             complement(291..650)
                     /locus_tag="VFH05_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679425.1"
                     /translation="MTHVALKACVLSVWVVLPPLDTVALERATIALSGPDCSLWHHAM
                     TEALEKHEGIARVEADTIPNHVLIDHDGRHSTDEELAALLNRLTVAHGCRAAVMKSCI
                     TAGSSTFTTPSTSHFSR"
     gene            complement(679..1551)
                     /locus_tag="VFH05_14215"
     CDS             complement(679..1551)
                     /locus_tag="VFH05_14215"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET6679426.1"
                     /translation="MKAVETEVVIEGSGEKYAAWTFDGQVPGPVVRVTEGDTVKFTLT
                     NPATNKYPHAMDFHAAEIDFLNNYKAINAGETISYTFVAKKPGIFFYHCGAPPMIQHV
                     ARGMFGAIIVDPKNASAWPKADREYVLIQSEYFKNADDVQAMFDRKFDGVMFNGGLFK
                     YHPFVSGGGKLDAKPGERVRIFFVNAGPNEFSAFHPIGEIWDNVYESGNPANKLSGVQ
                     TYVVGPGSAAIFDVVVESAGAYPLVTHSLTAALRGAIAVLVASPDAKPAPLMPMVPWK
                     ISVPQTEITPVPAH"
     gene            complement(1795..2544)
                     /locus_tag="VFH05_14220"
     CDS             complement(1795..2544)
                     /locus_tag="VFH05_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6679427.1"
                     /translation="MASERDPLSKPLTISSSKRPPASIDALLDRVPLFNILNRQDRQR
                     LLQELSETRYGKNDFIFREGEPSEYFHIVKEGTVKCVKSTSDGKECTLKMLMPGDLFC
                     CDAAAFDGANHPGNAQPMGNVSILRMHKSAYFNMLKRNPDAAIEVIKYLGNRLHEAQE
                     KNKILALDRADQRLAALLTDLATRNGIKERQGLRIPMRLTRQDMANMVGATTETVIRI
                     MSRFKRAHLVSGTANRLIILDFDRLRTLASG"
     gene            complement(2492..4357)
                     /locus_tag="VFH05_14225"
     CDS             complement(2492..4357)
                     /locus_tag="VFH05_14225"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6679428.1"
                     /translation="MPGTTLSHEQLTALRNTATHLRMESVRSTTAAGSGHPSSCCSAA
                     DIMAALFFSVMRYDPKNPKAPNSDRFVLSKGHAAPVLYAAWAEAGLFPKQDLLKLRTL
                     HSDLEGHPTPRLPFVDMATGSLGQGLPVGVGIALNAKHVDKLDYRTYVLMGDGESVEG
                     SVWEAVEVARQQLLDNLCAIVDVNRLGQSDPTMLQHDMESYRARWSGFGWHAVIVDGH
                     DLPAILGAFEEAARTKHKPTVLLAKTFKGKGISFMQDQPNWHGKPVPQGAEMQKAIDE
                     LTQQLQPNGTAPVIRRPSAPSASAPTITPLPAPPYKVGDSVATREAFGVALEALGAVN
                     PLVVALDADVKNSTYTEKFGKKFPNRFFENFIAEQSMLGSAAGLAACGKIPFAATFAA
                     FFTRAYDFIRMAAISQSNIKLVGTHVGVSIGEDGPSQMGLEDIAMMGAQPGVTVLYPS
                     DGTCTYRLMEIAANHKGLVYLRTGRPKSPVLYGPEERFHIGGCKVLRHSANDVLTIVA
                     AGVTLFEALKAHDQLKAKGVSVRVIDLYSIVPIDRTTLLESASATQGRLLTVEDHYAH
                     GGIGDAVLDAVGSEGIRVHKLAVRTIPHSGKPDELIDHYGIGTRSIVEATHHIIK"
     gene            complement(4692..5105)
                     /locus_tag="VFH05_14230"
     CDS             complement(4692..5105)
                     /locus_tag="VFH05_14230"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET6679429.1"
                     /translation="MAMTIPPLRLRHPDHALTLPFTTLRMLMLGLLLVVGWMLAGCGS
                     GDEGGPSASATLTWDPVDDVHGYYVYYGTESQGSSGSCSYPNKVFTSTPSAIVPGLAP
                     NTTYYFAVSAFNGIESECSAEHPSDSGDIDPVVIG"
     gene            complement(5525..5959)
                     /locus_tag="VFH05_14235"
     CDS             complement(5525..5959)
                     /locus_tag="VFH05_14235"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET6679430.1"
                     /translation="MKLSKKSEYALRALLELTLVHGKATLQRHEIAARQHIPVEFLEQ
                     ILLALKRAGLLSSRRGMRGGYGLLKSPEEVTLGQVIRVLDGPLAPIGCVSKTAYQKCR
                     DCPYTDNPRCPVQHVMGTVRDAIADILDHYTLADFAAGSPRG"
     gene            6177..7115
                     /locus_tag="VFH05_14240"
     CDS             6177..7115
                     /locus_tag="VFH05_14240"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET6679431.1"
                     /translation="MTFQELILTLNRFWADYGCVIQQPYDLEMGAGTFHPATFLRSLG
                     PEPWRAAYPQPCRRPTDGRYGENPNRMQHYYQYQVVLKPSPDNIQELYLDSLARLGID
                     PKKHDIRFIQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGIELSPITGEITYG
                     TERIAMYLQQVNNVFDLAWTDGMSYGDIHHETEVQFSRYNFEQGDVAMLMNAFQSYEA
                     ECKRLLAQSDQRLTLPAYDFCIKSSHAFNLLDARGAISVAERTGYIARVRALARQCAE
                     RYIEERASMGHPLIKHRQPAHSGTRPTDGVGRSTRR"
     gene            7112..>9084
                     /gene="glyS"
                     /locus_tag="VFH05_14245"
     CDS             7112..>9084
                     /gene="glyS"
                     /locus_tag="VFH05_14245"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET6679432.1"
                     /translation="MTRGKAPRGLSKVSSASGAELLFEIGVEELPYQVIAPTMSALKE
                     AAVRLLEEARLTSGSVNVYGTPRRLTLVISGLALRQASAKKEVMGPSKAVGVDSQGQP
                     TKAAIGFAAGQGLSVDRLEVRRTAKGDYLFAVKEEAGRPAQAVLLELLPQLVGKLSFP
                     KAMKWNETATRFARPVRWLLACFNKALLPIEAAGIRAGDRTYGHRVIGQRKALVVRNY
                     ESYRSGLKRAGVLVDPADRRQVIEQQLRQICSRAAVELNADESLMDQAVFTTEWPCAL
                     LGSLKSEYLSLPEEVLMTSMKEHQGFFSVRDRTTGKLAPHFIAVANNQPKDMSLIRAG
                     NERVLAARLADAKFFFDEDRRIRLEDRVKKLSGVTFHHKLGTMGQKAERLVKLVSLIA
                     AGVDRGSDSQTFVSACRRAAHLSKADLLTGIVGEFPELQGIMGGEYAKHDGEPEAVNQ
                     AIREQYLPKALEGELPHSLSGLVLSLADRLDTIAAFFHVGMIPTGSEDPFALRRHATA
                     IVRIVLEKELRLDLRKYLDHARTLIVDCGIKGTPDSEEHGSRRVTDFVFERLRHYGRT
                     VHAFRDDVMDAVLRPASAGPIDLLDLLSKLKAVQGVTAKPDFDPLIVGFKRASRIIEK
                     EQWDRKPVDAGMFKEAAEQDLHRQIRACQQDFE"
BASE COUNT         1980 a         2484 c         2681 g         1939 t
ORIGIN      
        1 attgggggat tgctttcgct cggcaccacg atttggggtg ccgtgatcgg ctcacgtgtg
       61 gttgccgagg tggaaacgaa acaaactggc acctaccggg ccgcataaga atgacccatg
      121 gttcgtatct ttctcactgg acaccgctgt tccccacatt cgtcagtaca tttcgctccc
      181 cttgaattct gtcgaagtgg cagaggatgt gtcgcattac acgcgcgatc ctcagcgacc
      241 gctcaagcga aaacgcatga tgctgatcgt tcaagggaac cgtccggcag ttatctactg
      301 aagtgactcg tgctgggcgt tgtaaacgtg gaggagcctg ccgtgatgca ggacttcatg
      361 accgcggcac ggcagccgtg tgcgacagtc agcctgttga ggagggcggc caattcttca
      421 tctgtgctat gacggccatc atggtcgatc aggacatggt tgggaatggt gtccgcctcc
      481 acccgcgcaa tcccctcatg cttctccaat gcctcggtca tcgcgtgatg ccacagactg
      541 cagtccggac ccgacaatgc gatggtcgct cgttcaaggg cgactgtgtc gaggggcggg
      601 agaacaaccc agacggacag aacgcaggct ttcaacgcaa cgtgggtcat agggagggcg
      661 tatgttgatg gtcggcgcct aatgggctgg caccggagtg atttcagtct gcggcacgct
      721 gatcttccac ggcaccatcg gcatcagcgg agccggcttg gcgtcaggcg acgcgacgag
      781 cacagcaata gcgcctctca acgctgcggt taacgagtgg gttacgagcg gatacgcacc
      841 ggcggactcg accacaacgt cgaatatggc cgcactaccc gggcccacca cgtaggtttg
      901 gactcccgac aatttgttgg ccggattgcc gctttcatag acattgtccc aaatctcgcc
      961 gattggatga aatgccgaga attcatttgg gccggcgttg acgaagaaga tccgtactcg
     1021 ttccccgggt ttcgcatcga gtttgcctcc gcccgaaacg aacgggtgat acttgaagag
     1081 accaccgttg aacatgacgc cgtcgaactt gcgatcgaac atggcctgaa catcgtcggc
     1141 attcttgaag tactcagatt ggatcaagac atattcgcgg tccgccttcg gccacgcact
     1201 ggcattcttt ggatcgacga tgatggcgcc gaacatgccg cgggccacgt gttgaatcat
     1261 gggaggtgcg ccgcagtgat aaaagaagat gcccggtttc ttggcgacga aggtgtagct
     1321 gatcgtttca ccggcgttga tggctttgta gttgttaagg aaatctatct ccgcggcatg
     1381 aaaatccatc gcgtgcggat atttgtttgt cgccggattg gtcagcgtaa acttaaccgt
     1441 gtctccctcg gtgaccctga cgaccgggcc tgggacctgt ccatcgaatg tccaggctgc
     1501 atacttctcg ccgctgccct ctatcacgac ctccgtttcg acagctttca tcccgatctc
     1561 atgagttttc gcgacaaccg tttccggcga tatcgcagtc aatactgtag aaatgctcag
     1621 ggcggcaaga ccaaaacctg gaaatgggcg catggacatc gacgggatcc tcctcagagc
     1681 aacggcaaat agagtccaga aaaatacagg ccatcctacg aaattgacct gggcgagcat
     1741 accgaagtgt cactgaccaa aacatgacca atgtcatgtt ttattgtggg ggcatcagcc
     1801 tgaggcgagt gttcttaacc ggtcaaagtc gaggatgatg agacggttgg cagttcccga
     1861 gaccaggtgc gcgcgcttaa agcggctcat gatccggatg acggtttcgg tcgtcgctcc
     1921 gaccatattc gccatgtcct gcctggtcaa tcgcatgggg attcggagtc cctggcgctc
     1981 cttgattccg ttcctagtcg cgaggtcagt caagagcgcc gccagccgtt ggtccgcacg
     2041 gtctaacgcg agtattttgt ttttttcctg cgcctcgtgc agcctattac cgaggtactt
     2101 gatgacttcg attgcggcgt cggggttccg tttcagcatg ttgaaatagg ccgacttatg
     2161 catgcggagg atgctgacgt tgcccatcgg ctgtgcgttg ccgggatgat tggcgccatc
     2221 gaacgccgct gcatcgcaac agaacaaatc acccggcatc aacattttga gcgtgcattc
     2281 cttgccgtcc gaggtggact tcacgcattt aacggtgcct tctttgacaa tatggaaata
     2341 ctcggacggc tcgccctccc gaaagatgaa gtcatttttg ccgtagcggg tttctgagag
     2401 ttcttgcaac agccgttgac gatcctgccg attcaggatg ttgaacagag gcacacgatc
     2461 gagcagtgcg tcgatcgagg cggggggccg cttacttgat gatatggtga gtggcttcga
     2521 caatggatcg cgttccgatg ccatagtgat cgatgagctc gtccggcttt ccgctgtgcg
     2581 ggatggtgcg cacggcgagc ttatggacgc ggatgccttc ggatccgacc gcgtcgagca
     2641 cggcgtcgcc aattccgccg tgtgcataat gatcctcgac cgtgaggagg cgcccctggg
     2701 ttgcggaagc gctttccaat aaggtcgtgc ggtcgatggg cacgatgcta tagaggtcaa
     2761 tgacgcgcac ggagactccc ttcgccttta gctgatcgtg agccttcagc gcttcgaaca
     2821 gcgtcactcc tgccgcgaca atggtcagga catcattcgc gctgtgccgc aggaccttgc
     2881 agccaccgat gtgaaaccgc tcctccggac catagagcac gggcgatttg ggtcgtcccg
     2941 tgcgcagata caccagtcct ttgtggttgg ccgcgatctc catcaagcga taagtgcacg
     3001 tgccgtccga aggatacagc acggtaacgc ctggctgagc gcccatcatc gcgatgtctt
     3061 ccaaacccat ttgcgaaggc ccgtcctcac cgatgctgac cccgacgtgg gttccgacaa
     3121 gcttgatatt ggattggctg atcgccgcca tgcggatgaa atcatacgct cgcgtaaaga
     3181 aggcggcgaa tgttgccgcg aacggaatct ttccgcaagc ggccaaacca gccgctgagc
     3241 caagcatgct ctgttcggca atgaagttct cgaagaacct attggggaac tttttgccga
     3301 acttttcggt gtacgtcgaa ttcttgacgt cggcgtccag cgcgacaacc agcggattga
     3361 ctgcccccag cgcctcaagc gcgacgccaa acgcttcacg tgtcgcgaca gagtcaccga
     3421 ccttataagg aggggcgggg aggggcgtga tcgtcggggc ggaagccgaa ggggcggacg
     3481 gtcgccggat aacaggcgcc gtgccattgg gctgaagttg ctgcgtcagc tcgtctatcg
     3541 ccttttgcat ctcggcaccc tgaggaaccg gctttccatg ccagttcggc tgatcctgca
     3601 tgaaggagat gcctttgccc ttgaacgttt tcgcgagaag caccgtgggc ttatgcttgg
     3661 ttcgagccgc ctcttcaaag gcgccgagaa tggctggcag gtcgtgacca tcgacgatca
     3721 ccgcatgcca cccaaaacct gaccaacgcg ctcgatagct ttccatgtca tgttgcagca
     3781 tcgtaggatc gctttgaccg agccggttga catcgacgat agcacacaga ttgtccaaca
     3841 gctgctgtcg cgcgacttcg accgcctccc agactgagcc ttcgaccgat tcgccatcgc
     3901 ccatcaacac atacgtacga taatcgagtt tgtcgacatg cttagcgttc agcgcaatgc
     3961 caacacctac gggtaatccc tgtcccaggg aaccggtagc catgtcgaca aatggaagcc
     4021 ggggagtggg atgtccctcc aggtcggagt ggagcgtgcg gagtttcagg agatcttgtt
     4081 tggggaagag tccggcttca gcccatgcgg catacagcac aggggcggca tgaccttttg
     4141 agagcacgaa tcggtcgctg ttaggggcct tcgggttctt cggatcatag cgcatgacag
     4201 aaaagaagag ggcggccatg atgtctgcgg cggagcagca gctcgaagga tgaccgctgc
     4261 cggcagcggt ggtcgatcga acgctttcca tgcgcaaatg ggtcgcggtg tttcgtaggg
     4321 cggttagttg ctcgtgagat aaggtggttc cgggcatgtg ctctccttgg tgtcggaggg
     4381 taatcgcaga aataaccgga ccgccctgtg agtggacggc tcctcttccg tgaaatctga
     4441 agctaacaaa ccgagggcga aggagtcaac gaagacgcac ggagtccggg cgagacgacc
     4501 aggctccgcg tgaggaagag aataaaatgg cgcgtgcgtc gtggtccgac atgaggatgt
     4561 tctggaagtg actcagttga ctcggtgcgt tctcatggtc cgcagacgat ctcctcagcg
     4621 aagcagcaac ctgttgccga ggtcgtcgga gctcgtgacg acctcggggc aacgtcttca
     4681 ctctgatggg atcagccaat caccacggga tcaatatcac cgctatcgct cgggtgttcg
     4741 gctgagcact cactttcgat accgttgaag gcgctcacgg caaagtagta cgtggtgttc
     4801 ggcgcgagcc cgggcacgat ggcggagggc gtcgatgtaa atactttatt cggataggaa
     4861 caggagccag aagaaccctg agactccgtt ccataataca cataatatcc atggacatcg
     4921 tccaccggat cccaggtcaa ggtcgcagag gctgagggcc ccccctcgtc tcctgagccg
     4981 caaccggcga gcatccaccc aaccaccagc agtaggccaa gcatcagcat cctgagcgtt
     5041 gtgaacggaa gtgtgagcgc atggtcgggg tgccgaagcc gcagcggtgg aatggtcatg
     5101 gccatagagt cagctccttt atgaacgtct cgagttggta aaattcggac tcgactggat
     5161 gtgagtgaag aaaccagcag gccgaaactc tgtggcgggt gatagcaata ccgatgcctt
     5221 ctcgagcgtg tggccggatg gcctgcaatc aactacttat gaagatttcc tgccgagggc
     5281 tgtctagaag gcagcaggcg atgaaggttg tgcccttcga agtgcatgcg aaattgaaca
     5341 cagtgctcgc tgaaggcggg taaagaccgt gaagagctcg agtaaaaacg cggaagaggc
     5401 gcagtaaaga ttcgtcgcgg actattcacc ccagactggc cgatcacaca taattagata
     5461 caccaccaat ggccagcggg cacaaggtac agaggtgcgg actcagagca aggaacactt
     5521 gtgatcaccc ccgaggggat ccagcggcga aatccgcgag tgtatagtga tccagaatgt
     5581 ctgcgatcgc atccctgacg gtccccatga catgctggac ggggcagcgc gggttgtcgg
     5641 tatagggaca gtcacggcat ttttgataag ccgtcttgct gacacaaccg atcggcgcga
     5701 gcggtccatc gagcacacga atgacctggc cgagcgtgac ctcttcaggt gatttaagca
     5761 atccgtaacc gccacgcatt ccgcgccggc ttgataggag gccggcacgc tttaaggcga
     5821 ggagaatttg ctcgagaaac tccaccggaa tgtgttgccg tgcagcaatt tcgtgacgtt
     5881 gtaacgtcgc cttgccatgc acgagcgtga gctcgaggag agcgcgaagg gcgtactcac
     5941 tttttttcga caacttcact ggaatccaag taaatcaatc aggctgcgca caatagatga
     6001 cgcttccatc acgcgtcaag gttcgcagcc cagagggccg attccattca cggtggacct
     6061 caactgcaac tccttgatct gtagacgttt gttgacagta tccggtggtt cctgttagct
     6121 tcgaacctca tttttctgca ctcctagacg cctctgaatg acggttggac tgcttcgtga
     6181 ccttccagga acttatcctc acactcaaca ggttttgggc cgactatgga tgcgtaatcc
     6241 agcagcccta tgacctggag atgggggccg gcaccttcca tcccgccacc tttttgcgat
     6301 cgctcggtcc ggagccctgg cgtgcggcct acccgcagcc ttgtcgtcga cctacggacg
     6361 gccgctacgg cgaaaatcca aaccgcatgc aacactacta tcagtatcag gtcgtgctca
     6421 aaccttctcc ggacaacatc caagagttgt atctcgacag ccttgcccgg cttggcattg
     6481 atcccaaaaa gcacgacatt cgatttatcc aggacgactg ggagtcaccg acgctcggag
     6541 cttggggatt gggatgggaa gttcgtttgg atggaatgga aattactcaa ttcacgtatt
     6601 tccaggagat aggcggaatc gagttgagtc cgattaccgg agaaatcacc tacggcaccg
     6661 agcgtatcgc gatgtacctc cagcaggtca acaacgtgtt cgatctggct tggacggacg
     6721 gcatgagcta cggagacatt caccacgaga ccgaagtgca gttctctcgc tataactttg
     6781 agcaaggcga tgtcgcgatg ctgatgaatg ccttccaatc gtatgaggcc gaatgtaaac
     6841 ggttactcgc ccagagcgat cagcggttga cgctgccggc ttatgacttc tgtattaaat
     6901 cttctcatgc cttcaatctc ttggatgccc ggggagcgat cagtgtcgcc gagcgcaccg
     6961 gctatatcgc ccgcgtccgg gcgctggccc gacaatgtgc cgagcgatat atcgaggagc
     7021 gggcttcaat gggccatccg ctcatcaaac accgccagcc ggctcattcg gggacgcggc
     7081 cgacagacgg ggtgggccgg agcacccgac gatgacgcgc ggaaaagcgc caaggggtct
     7141 atcgaaggtg tcttctgcca gcggcgccga gctcttattc gaaatcggcg tcgaggaact
     7201 tccctaccaa gtgattgctc ccacgatgtc ggcattgaaa gaggcggccg tccgtcttct
     7261 cgaagaagcc aggctgacaa gcggatcggt taatgtgtac ggcacccctc ggagattgac
     7321 cctcgtcatc agtgggctcg cgcttcgtca ggcgtctgcc aagaaagaag tgatgggtcc
     7381 ttcaaaagct gtgggtgttg attcgcaagg gcaacccacg aaagccgcga ttggattcgc
     7441 cgctgggcaa ggtctgtctg tcgatcgcct cgaagtacga cgaaccgcga aaggtgacta
     7501 tctgttcgcc gtgaaggagg aggcggggcg accggcgcaa gcggtgctgt tggaactcct
     7561 tcctcaactg gtaggtaagc tctcgtttcc caaggccatg aaatggaatg aaaccgcgac
     7621 caggtttgcc aggccggttc gttggctcct cgcttgcttc aataaggccc tcctgccgat
     7681 cgaagcggca ggaatacggg cgggcgaccg gacttacggg cacagggtga tcgggcagcg
     7741 caaggcgctt gtcgttcgga actatgagtc ctatcgcagc ggtctgaaac gggcgggtgt
     7801 gctcgtcgat ccggcagacc gtcgacaagt catcgagcag cagctccggc agatctgctc
     7861 aagagccgcg gtcgagctca acgccgacga atcgttgatg gatcaggccg tctttacgac
     7921 cgaatggccg tgcgccctgc tcggcagttt gaaatcggaa tatctcagcc tgccggagga
     7981 agttttgatg acctctatga aagagcatca gggattcttt tcggtgcggg acagaacaac
     8041 tgggaagctc gcgccgcatt ttatcgcggt ggcgaacaac cagcccaaag atatgtcgtt
     8101 gattcgagca ggaaacgagc gtgtgctcgc agccagattg gccgatgcca agtttttttt
     8161 cgacgaggat cgcaggatac ggttggaaga cagagtgaaa aagctcagcg gtgtcacctt
     8221 ccatcataag ctcggcacga tgggacagaa agcagaacgc ctggtcaaac tcgtgtccct
     8281 catcgccgca ggtgtcgatc gcggctcgga tagccaaacc tttgtgagtg catgccggag
     8341 agcggctcat ctcagtaaag cggatctcct cactggcatc gtcggcgaat tcccagagct
     8401 acaagggatc atgggtggtg agtacgcgaa gcatgatgga gagccggagg ccgtgaatca
     8461 ggcgattcga gagcagtacc tgccgaaggc gctggaaggg gaattaccgc actctctttc
     8521 cggactggtc ttgtcgcttg ccgaccgact ggacaccatt gccgcatttt ttcatgtcgg
     8581 catgattccg accggctcgg aagatccctt tgccctccgg cgtcatgcca ctgcgatcgt
     8641 gcgcattgtt ctggaaaagg agcttcgcct ggaccttcgg aaatacctcg atcacgcacg
     8701 gacactcatt gttgactgtg gcatcaaggg aacgcctgat tccgaagagc atggaagccg
     8761 gcgagtgact gattttgtct tcgaacgcct gcggcattac gggcggacag ttcacgcgtt
     8821 ccgcgacgac gtaatggatg ccgtcttgcg accggcttca gccggaccga tcgatctgct
     8881 cgatctcctg tcaaagctga aagcagtgca aggggtgacg gcgaagccgg atttcgaccc
     8941 actcatcgtc ggttttaagc gcgcttctcg gattattgag aaagaacagt gggatcgaaa
     9001 gccggtcgat gctggaatgt tcaaggaagc ggctgaacaa gatttgcatc ggcaaatccg
     9061 tgcctgtcag caggacttcg aaca
//
LOCUS       DASSCQ010000226         7906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_112754, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000226 DASSCQ010000000
VERSION     DASSCQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112754"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFH05_14250"
     CDS             <1..855
                     /locus_tag="VFH05_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679433.1"
                     /translation="GGIILSQSDAKILCEQTREWERSLEMSTGELVLGEWQEYIKRFG
                     HVLLWAFASLRFSAVADALTHIQYRTPEKEPYLYAIAMYDHHDYQLFAQGLSGIEGVE
                     PETPARPTRKDTPDSNIASSRSWVLRHTGAQATAIATRFTLTSSQLFVECDSPERLNH
                     MKHQLARTFGFSLHFRQETVTPPTIRFPIDQLMSEQPLTVVVTQEEDLALLKAFLEKA
                     YLEWSDQGHAMLSGKTPRHASASMTMRQSVIDLIDEMERNDLGLRRVGHRAYDYNVLR
                     GHVGLEEV"
     gene            1010..2389
                     /locus_tag="VFH05_14255"
     CDS             1010..2389
                     /locus_tag="VFH05_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6679434.1"
                     /translation="MFNIVRNVLVVDDDHEMRALLRDVLEEHGYTVTLASNGQEALAS
                     LRDREYPVVLTDLRMKGMQGIELLTQIKQSWADTNVILMTAFGSVETAIQAMKQGAYD
                     YLMKPVKNDDLLRATERGFREALLRSEINRLRREVGKEFSFSQILGKSKPMREVFELI
                     RRVANSPTNILITGESGTGKELVAKAIHYNSDRKDAPFIPVNCAAIPDQLLESELFGH
                     MRGSFTDAKGDKHGLFEEAHKGTLFLDEISELPITLQAKLLRAIQEKEIRRIGATKPI
                     SVDVRIIAATNLHLAEEVKAKRFREDLYYRLNVIEVKLPPLRDRREDIPLLVDGFLKK
                     CGTTRGKEVKGVTEAALAMLMDYMWPGNVRELENVVERAVTLSRGEKITPDDLPPGVQ
                     GARGDRRVLEEAAEKTLPLHEIEKEYIKKILEKTGGNKYQAAQVLGIDRKTLYRKLAE
                     IEGTPQPED"
     gene            2423..4009
                     /locus_tag="VFH05_14260"
     CDS             2423..4009
                     /locus_tag="VFH05_14260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6679435.1"
                     /translation="MQVPIEIVPRHARWMILLLTVGIFVVDLLTPVGLAVSVLYILPL
                     LLTFFSERQRDPLYFFFAATVLLWADLAWKPAALVLPYSVMNRALGTFVLWLVASGLV
                     MLKRMQSDLLNAEVTKTKVMQDLLSERIERSHAEGLMEAAQQARAHAETAVMGAVAGR
                     QRAEEQYMLSKLRLQGIIESAMDAIITVDYDQKVVVFNDAAERMFQCSSQDALGQPLD
                     NFIPPRYREAHRQHVKGFGQSGVTSRRMGQLGTVMGLRADGVEFPIEAAISHVTVEGK
                     HFLTAILRDVSERTRLQEQLRRTERVAELGTLASGMAHEIGTPMNVILGRAEYLLDRV
                     SDEPVKKGLQTIVAQVERITRVMNQLLTFARRKPPEPVPVDLQEVIENSIEMFQERLA
                     NHHVQLARHIDPNCPRIVADSDQMNQVLINLIMNAIHAMPQGGILRFGLDCERDIVKL
                     TVSDTGHGISKEVVNKVFDPFFTTKEFGKGTGLGLTVVKGIVEEHQGSIQVESEEGKG
                     TTFTIHLPTSQSGPQGTHDR"
     gene            complement(4055..4555)
                     /locus_tag="VFH05_14265"
     CDS             complement(4055..4555)
                     /locus_tag="VFH05_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HET6679436.1"
                     /translation="MASKLKSPAGHGPRRDRTVQEYQHDTYKLRGKLKEPTVCSGCGA
                     LFHKGRWTWGFVPKDATPTTCPACLRAHDRYPKGLVTITGKLNESQQEQVRGVIKNTE
                     AREIREHPLSRIMGIERRPEGLVISTTDTHLPRRIGEALKHAYHGELELHYDHDEDFV
                     RVNWRR"
     gene            complement(4621..5166)
                     /locus_tag="VFH05_14270"
     CDS             complement(4621..5166)
                     /locus_tag="VFH05_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6679437.1"
                     /translation="MNRVLFRVGCVFVFTPCLWIGLSMPAEAKTQLMPGGIQEVDSAT
                     LKDLVSTFEQAELAMQAHDLERIMALYSDDYLYHGLKKADVQKIWKQLFDHYKELESF
                     HSFSVVRLVGSGSKLVAEMTCSGVVWGTSKDTTLRSPVDSWYEEIHYLKKENGRWRIV
                     GNVGGESIPVLQFGVAPHPLF"
     gene            complement(5698..6369)
                     /locus_tag="VFH05_14275"
     CDS             complement(5698..6369)
                     /locus_tag="VFH05_14275"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6679438.1"
                     /translation="MHLLSYPDVSRRPRFGVVAASILTASLFAVSYAIGVGTAEEKGE
                     SPTEAVRETLTEIFRILEDPKLKEPAQQARRRHMLEESIGRRFDYGEMSKRTLASHWS
                     RLTDDERTQFVAVFKGFLSDRYAGKIEGYSGEKVQYLGERLEGQYAEVRTKLVSSKVE
                     YPLDYRLINKAGRWHAYDIVADGVSLVRNYRSQFDKILKTGSYEELISRLQNRSVPEA
                     PKEKR"
     gene            complement(6381..6584)
                     /locus_tag="VFH05_14280"
     CDS             complement(6381..6584)
                     /locus_tag="VFH05_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET6679439.1"
                     /translation="MSVAKIIEISCESPKSFEDAIQGGIMRASKTIHNIKGAWVKEQQ
                     VVIENNKITMYRVDLKVTFVLDQ"
     gene            6896..>7906
                     /locus_tag="VFH05_14285"
     CDS             6896..>7906
                     /locus_tag="VFH05_14285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6679440.1"
                     /translation="MNPVRPLYVPPLLYDKHESASLILRDGSTAMIRLADQNDAILMQ
                     EFVGRLSPESKHHRFFSESTPSSDLIRTLCDSSNPRSQLTLIVTRVFDGVFRIIASGS
                     YWARDMHIAEVAMAVDDQFHGKGLGTLLVERLALLAIRNGFTRLWAVTHAENLAMREV
                     FRESGFTAHEVYESGDIEVELSLIPTMTTVIRAELRERLATIASLRPFFHPRGVAMIG
                     ASRDTRSIGYRLLEALIANRFQGSIYPVNPFATEILGLRVYPSLRQVSEPIDLAVIAV
                     PRDQVIGVVEDCAIHGVRALVIVTAGFAETGVKGAALQEELLRKVRENGMRMIGPNCF
                     GLL"
BASE COUNT         1810 a         2164 c         2157 g         1775 t
ORIGIN      
        1 ggcggcatca ttctctcgca gtccgatgcc aagattctct gtgaacagac gcgagaatgg
       61 gaacgcagcc tggagatgtc caccggcgaa ctggtgctcg gcgagtggca ggagtacata
      121 aaacggttcg gccatgtcct gctctgggcc tttgcttccc tccgcttttc agccgtagcc
      181 gatgcgctca ctcatattca ataccgtact cccgagaaag agccctatct ttatgccatc
      241 gcaatgtatg atcaccacga ctatcaactt tttgctcaag gtctttctgg aattgagggc
      301 gtggaaccgg aaacgccagc caggccgaca cgaaaagaca ctccagactc caatattgcg
      361 agttcacgat cgtgggtcct gcgtcacaca ggagcgcaag cgacggctat tgcgacgagg
      421 ttcacgctca cttcttccca actcttcgta gaatgcgata gtccggagcg actgaatcat
      481 atgaagcatc aactggcgag aacgttcggc ttttcattac atttccggca agaaaccgtc
      541 acgccgccaa cgatccgctt ccccattgac cagctcatgt ctgagcagcc actgactgtg
      601 gtagtgacgc aggaggaaga tctggccttg ctcaaagctt ttcttgaaaa ggcatacctt
      661 gaatggtcag atcaagggca tgcaatgttg agtgggaaaa cgcctcgcca cgcatcagca
      721 tcgatgacga tgcggcaaag cgtcatagac ttgattgacg agatggaacg aaacgatctt
      781 ggcctacgac gagtgggcca ccgagcgtac gactataatg tcctgcgggg gcatgtagga
      841 ctcgaggagg tatgactgtc aagacaatgc cccgcgatgt cctccgctga acaccgggaa
      901 gcgtaagata ctcgcagagc acgcatgcgc agagagattc tggcgttcta gaaccactgg
      961 atttgcccac ctcattttga tattagtggc tcccttatcg gagattccta tgttcaacat
     1021 cgtccgcaat gtgctggtag tcgacgatga tcacgagatg cgggccttgc ttcgggacgt
     1081 gctggaagaa catggctata ccgtgacgct cgcctccaat ggacaggaag ctctcgcgag
     1141 tctccgagac agggaatatc cggtcgtgct cacggattta cggatgaaag ggatgcaagg
     1201 gattgagctc ctcacccaga tcaaacaatc gtgggccgac accaacgtaa tcctaatgac
     1261 cgccttcgga agcgtagaaa ccgcaatcca agccatgaag caaggtgcct acgactatct
     1321 gatgaagccg gtgaagaatg acgatctcct gagagccacc gagcgtgggt tccgtgaagc
     1381 gctgctgagg agcgaaatca accgcctccg gcgtgaagtg ggtaaggagt ttagcttcag
     1441 ccagattctt ggaaagagca agccgatgcg agaggtgttc gagttgatcc gccgggtcgc
     1501 gaacagtccg accaatatac tgattacggg agagagcggg acaggcaagg aactcgtggc
     1561 gaaggcgatt cactacaaca gcgatcgtaa ggacgcccct ttcatccccg tcaactgtgc
     1621 tgccattccc gaccagctct tggaaagcga gctgttcgga cacatgcgag gatcgtttac
     1681 cgacgccaag ggagacaagc acggtttatt cgaggaggcg cacaagggca cgctgtttct
     1741 ggacgaaatc agcgaattgc ccatcacctt gcaggccaaa ctgttgcgtg cgatccaaga
     1801 gaaggaaatc cggcggatag gcgccacaaa gccgatctcg gtggatgtgc ggatcatcgc
     1861 agccaccaac ctccatctag ctgaagaagt caaagccaag cgattccgtg aagatttgta
     1921 ctatcgactg aatgtcatcg aagtaaagct gccgccgctc cgagatcgcc gagaagacat
     1981 tccgttactg gtggacggct ttctcaagaa atgcggaacg acgcgcggca aagaagtgaa
     2041 aggcgtcacg gaagccgcgt tggcgatgtt gatggactac atgtggccgg gaaatgttcg
     2101 cgagttagag aatgtcgtcg agcgggcggt gacgctcagt cgaggagaaa aaatcacgcc
     2161 cgacgatctc ccccctggcg tgcagggggc acgaggcgac cgacgtgtgt tggaagaagc
     2221 tgcggaaaag acattgccgc tgcatgagat cgagaaggaa tacatcaaaa agatccttga
     2281 gaaaacggga gggaacaagt accaagccgc ccaggtgctc ggcatcgatc gcaaaacact
     2341 gtatagaaag ctcgcagaaa tcgaagggac gccgcagccc gaagattgac aacgttcagg
     2401 ttattcggcc gggatcgtgc ctatgcaagt tcccattgag atcgtgcccc gccatgcgcg
     2461 ctggatgatc cttctcctga ccgtcggcat ctttgttgtc gatctgctga ctcccgtcgg
     2521 actagctgtc tcggtcctct acatacttcc ccttcttctg acgttttttt ccgaacggca
     2581 gcgcgatccg ttgtatttct tcttcgctgc gaccgtgctg ctttgggccg acttggcctg
     2641 gaaaccggcc gccctggttc ttccatacag cgtgatgaac cgggcattag gaacgttcgt
     2701 gctttggctt gtcgcatcag gcttggtcat gcttaagcgg atgcaatcag atctgctgaa
     2761 tgccgaggtc accaagacga aggtcatgca ggacctgctg tcagaacgta tcgagcgatc
     2821 gcacgccgaa gggttgatgg aggcagcaca acaagcgagg gcccacgcgg aaacagccgt
     2881 gatgggcgcg gtcgctggcc gacaacgggc cgaagagcaa tacatgctca gcaagctccg
     2941 gctccaaggc attatcgaat ccgcgatgga tgcaattatc acggtcgatt atgaccagaa
     3001 ggtcgtcgta ttcaacgatg ctgccgagcg catgttccaa tgctcctctc aagacgccct
     3061 cggccagcca ctagataatt ttattccgcc tcgttatcga gaagcccatc gtcagcatgt
     3121 gaaagggttt ggacagtcgg gggtcacgag ccgaagaatg ggacaactcg ggacggtcat
     3181 gggtctgcgc gcggatggcg ttgaatttcc catcgaggcc gccatttccc atgtcactgt
     3241 cgaaggaaaa catttcttga cggccattct tcgcgacgtg tctgaacgca cacgattgca
     3301 agaacagctc cggcggaccg agcgcgttgc tgagctggga acgttggcgt ccggcatggc
     3361 gcatgaaatc ggcacgccga tgaacgtcat cctcggtcgc gccgaatatc ttttggatcg
     3421 ggtcagcgac gagccggtca agaaagggct gcaaacgatc gtggctcagg tcgagcgaat
     3481 caccagagtg atgaatcaac tcctaacatt cgcccgaagg aagcctccag agcctgtccc
     3541 cgtagatctg caagaggtga tcgagaacag catcgagatg ttccaagagc gattggccaa
     3601 tcatcacgtg caactagcta ggcacatcga tcccaattgc ccgcgcattg tggcggacag
     3661 cgatcagatg aaccaagtgt tgatcaatct catcatgaac gccatccatg ccatgccaca
     3721 gggtggcatc ttacgatttg gactcgattg tgagagggac atcgtgaagc tcaccgtctc
     3781 cgatactggc catggcatat cgaaagaggt cgtcaacaag gtcttcgacc ccttctttac
     3841 aaccaaagag tttggaaaag gcaccggctt agggttgacg gtggtgaaag gaatcgttga
     3901 ggagcatcag ggttcgatcc aggtcgaaag tgaggaggga aaaggaacca catttaccat
     3961 tcatctcccg acgagccagt cagggccgca agggactcac gaccgatgac gtctgctttt
     4021 agctaccgga ctcgctcgca tcatacgccc ggcgtcatcg ccgccaattc acccgtacga
     4081 aatcttcgtc gtggtcataa tgcagctcta gttcaccgtg ataggcatgc ttcaaggctt
     4141 cgccaatccg ccgtggcagg tgcgtgtcgg tcgtcgaaat cacaagtcct tcgggccggc
     4201 gctcgatgcc cataattcgt gagagcgggt gctctcggat ctctcgggct tccgtgttct
     4261 tgatgacccc tcgcacctgt tcctgctggc tctcattgag tttgcccgtg atcgtcacca
     4321 gacccttggg atatcggtcg tgggcacgaa gacaagccgg acatgtggtg ggtgtcgcat
     4381 ctttcggcac aaatccccat gtccatctcc ctttgtgaaa cagcgcgccg cagccgctgc
     4441 ataccgtcgg ttccttgagt ttcccgcgca acttgtaggt gtcatgttga tattcctgga
     4501 cggtccggtc ccggcgcggt ccatgaccag ccgggctttt caatttgcta gccattgctt
     4561 cacctttcct cgtcgaggtg tcgtcacaga tgtccctgtg catcaatgtg tctcatgcct
     4621 ttagaacagc ggatgcggtg cgaccccaaa ctgcaagacc ggaattgatt ctccgccgac
     4681 gttgcccaca atccgccagc ggccgttttc tttcttcaaa taatggattt cctcatacca
     4741 gctgtcgacc ggcgaccgca atgtcgtgtc tttggaggtc ccccagacca cgcctgaaca
     4801 cgtcatttcg gcaaccagtt tgctgccgga ccccaccaaa cgaacgaccg agaacgagtg
     4861 aaaactctcg agttccttgt aatgatcaaa aagctgcttc caaatcttct ggacgtctgc
     4921 ctttttgagt ccatgataga gatagtcgtc cgagtacagc gccatgatgc gttcgagatc
     4981 gtgcgcctgc atagccagct ccgcctgctc aaacgtcgac acgaggtcct tgagggtcgc
     5041 cgaatccact tcctgaatcc cgcccggcat caattgagtt ttcgcctcgg caggcattga
     5101 aaggccaatc cacaagcacg gcgtgaatac gaacacacaa ccgactcgga atagcactcg
     5161 attcatgaac aggcctcctt tgtgtgatgc gctgccaatg tgagcatggg ttctgcaagg
     5221 tagagaccga ggatgtgcct tcgatcgagg accttgcgat tcgcttgttg aatcgaacag
     5281 tccgacgcgg tcgatcgcct gaagcgcttt gggtcaactg tggcagaaat cagcagtgaa
     5341 gaaggatggc cggtggaaaa tcgcaccagc gcggtcaagg atgtagtctt cgtggagcgg
     5401 cggggcgcga cttccacaag accgctgttt tttatgtggg gtttccgcct accactaaca
     5461 ggcctcaaaa aggaatgctg gcttccggcc cgaaggattc taccattcag aacactgagg
     5521 aagagcggag acgggtctgg agagcttcgc tcgaattgtg aggccttgga ggggttttag
     5581 gcttcccgct cttctggcgg gcttgctcac cacccctgcg gctcggcctc tctatcagga
     5641 gtgttcctct caccgaggcc tccgctcaat tagcagatag cgcctctttt cgacgagtca
     5701 acgcttttcc ttcggtgcct cgggtaccga tcggttttgc aagcggctga tgagctcctc
     5761 atacgagccg gtctttaata ttttgtcgaa ctggcttctg taattgcgca cgagactcac
     5821 gccatccgct acgatatcgt aggcatgcca gcgccctgct ttgttgatca atcgataatc
     5881 caggggatat tcaactttgc tcgacacgag ctttgtgcgg acctccgcat attggccctc
     5941 cagccgttcg ccgagatact gcactttctc gcccgagtac ccttcgatct ttccggcgta
     6001 gcgatccgac agaaagcctt tgaagaccgc cacaaattgg gtgcgttcgt cgtccgtcag
     6061 gcggctccag tgagaggcca gggtccgttt ggacatctcc ccataatcaa accgacggcc
     6121 aatggactct tccagcatgt ggcggcgtct tgcctgttgg gcaggctcct tgagcttggg
     6181 atcctcgagt atccgaaaaa tctctgtgag cgtttccctg actgcctcgg taggggactc
     6241 gcccttttcc tctgccgttc cgactccaat ggcatacgag acagcgaaca gcgaagcggt
     6301 gaggatcgag gccgccacca cgccgaaccg cggtcggcgg ctcacgtccg gataagacag
     6361 caggtgcata cggaagactc ctattgatcc aacacgaacg ttaccttcag atcgacgcga
     6421 tacatcgtga tcttattgtt ctcgatgacc acctgctgtt ccttgaccca ggcgcctttg
     6481 atgttgtgaa tggtttttga ggcgcgcatg atcccaccct gaatcgcgtc ctcaaagctt
     6541 ttaggcgact cgcaactgat ctcgatgatt ttggcaacgg acatgggaga accctccata
     6601 tgtggatggt taagaaaagt ggtcaactcc tctgcagcaa gcaatcgata tgccatcctg
     6661 cccagagagt ttagaattgg acatccttct aagacagctc aacgctatgt cagtttttcg
     6721 atttcaggcc ttgaataacg gcgcagtggc gctcaaacac ccgatcttcg gcagacagtt
     6781 gattgtctaa aaggccgtcg agccaagccg atgcgacagg cattccctta tttcgtgtgg
     6841 caggcaaaca gtgttatatg aagcacaatg gcatttacaa gcacccaagg atcgtatgaa
     6901 ccctgttcgc ccactctatg tcccgcctct cctgtacgat aaacacgagt ctgcatcact
     6961 gatactccgg gatggatcaa ctgccatgat ccgtctggcc gatcagaacg atgccatact
     7021 gatgcaagaa tttgtggggc gtctctcacc agagtccaaa caccataggt ttttctccga
     7081 gagtacgccc tcttccgatc ttattcggac cttgtgcgat tcatccaatc ctcgttctca
     7141 gctcacgcta attgtcaccc gggtctttga tggcgtcttt cggatcatcg cgagcggctc
     7201 ttattgggcg agggatatgc acattgctga ggtagcaatg gcggtagacg accagtttca
     7261 tggcaaaggc ctggggacac ttctcgtaga acggctcgct ctgcttgcga ttcgaaatgg
     7321 gtttacacgt ctgtgggcgg tgactcatgc agagaatctg gcaatgcgcg aagtcttcag
     7381 agaatcaggc ttcaccgccc atgaagtcta tgaaagcggc gacattgaag tggaattatc
     7441 attaatcccc accatgacga ccgtcatacg ggccgaactg cgggaacggc tcgcaaccat
     7501 tgcctctctc cgaccgtttt tccacccacg aggtgtggca atgatcgggg catctcgtga
     7561 tacccgcagc attggctacc gtctactgga agcgctgata gccaatcggt ttcaaggttc
     7621 aatctatccc gtgaatccct ttgcaactga gattctggga ctgcgagtct atccatcttt
     7681 gcgccaagtc tccgaaccta tcgatttggc cgttattgca gtgccgcgtg accaggtaat
     7741 tggcgtggta gaggattgtg cgatccacgg agttcgagcg ctggtgattg tcacggctgg
     7801 attcgccgaa actggggtga aaggtgcggc ccttcaggag gaattactcc ggaaggtacg
     7861 tgaaaacggc atgcggatga ttggcccaaa ctgctttggt ttgttg
//
LOCUS       DASSCQ010000227        23951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1603081, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000227 DASSCQ010000000
VERSION     DASSCQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 23951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23951
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603081"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..2181
                     /gene="glgP"
                     /locus_tag="VFH05_14290"
     CDS             25..2181
                     /gene="glgP"
                     /locus_tag="VFH05_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET6679441.1"
                     /translation="MNTTSIDPVRTHIPTSLARLPELSQNLWWSWTVEARQLFETIDP
                     TLWFYTHHNPVKLLADIQPERLAKLAEDPSYLRQYSAVLKLFDEYRSNGRSWFTMQHA
                     SETKKVIAYFSAEFGLHTSIPIYSGGLGILAGDHCKEASDLGVPLVGIGFMYPQGYFR
                     QRITAEGWQEAAYAPFNREDSPIHPALTPSGEACRFTVDMGGRNVTAVVWKVLVGRIP
                     LYLIDTDVPENSAENRALSARLYGGDQEMRLCQEILLGIGGVRLLRALDIAPSVWHAN
                     EGHSAFLTLERLRELVQKGLSHAEASDMVRQSTVFTTHTPVPAGHDVFPHYLMDRYFA
                     GYWEQLNLSRDEFLGLGETPESRGHGFNMTALVMRLSAHVNGVSREHGRVSREMWQHF
                     WPGLPVEEVPIRSVTNGIHAPTWIAQDLHRLYSKSLHPAWSDHCDDPAMWQRVLDIPD
                     HELWAVRQTMKRKLMGFIRERARTGWMQGNLQPSQVLSRGTLLDPEALTIGFARRFAT
                     YKRATLLFQDLDRLKRLLQDKWRPVQLVFAGKAHPADEPGRYFIHEVMNFCHDHKLGG
                     HVAFLEDYEMHMAKYLVQGVDIWLNTPRFPMEASGTSGMKAALNGVLHFSTLDGWWTE
                     GYNGANGWGIQPLQGSQDLQAQDSHDAEQLYRQLEQEVVPLFYQRDLDGIPRGWLQMV
                     KESIRTIAPQFCTKRMVKDYVETLYCPAMTRTPSSW"
     gene            2210..3538
                     /locus_tag="VFH05_14295"
     CDS             2210..3538
                     /locus_tag="VFH05_14295"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6679442.1"
                     /translation="MLLAFAVPPDVVASTATSSEREAKDAFRRADYGTVLKLLPPASS
                     GITPSKAWLRLAVDSAIHVGRPEEGLEIYDRLVKPGQPEEVPLLQRLGIGFLRAYVRD
                     SREHLRIAAYSALAELNLPDQQPVLEDGLLDASPIVRARAIEALGRAGLGARSQALRR
                     AMQDEIPSVRIAAMNVLSEAQITEIIPHVEKVARTEDGPEGIFAYAALYRLGNRDMLV
                     DITGAATLPDRETRMAALGVLGRLKHSSSLAVVSQGVYDPEPQVRAFAAGALGELGQP
                     GAVAPLTHALGDENPRVRGAAAASLGRLGVSDARPLLHALVRDPSMQVRASAVEGLLR
                     LGDQTAVLVATDLARHPDPSVRAAAAQALGAGSDRQALAVLQTLLQDQQPQPRLFAAK
                     ALGKVSLPVLPLIKKALHDSDAAVRITSAGSLLRQLEPVAKSSPRRGRKG"
     gene            3541..3996
                     /locus_tag="VFH05_14300"
     CDS             3541..3996
                     /locus_tag="VFH05_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679443.1"
                     /translation="MWRFVGALVIAFAAFGLGYYFGQRPGGTLEQTVGDLQHSFKDMS
                     RNVVDTTMGIERDLRRRQALLDTKSRVVQAKSELFDKNFGEAAKQLAEGVDALESSVK
                     GAKPNEQTQAVRGLIGTLQEARLELTMGKPVPIRKLTELQQSIDRELNR"
     gene            complement(4000..4830)
                     /locus_tag="VFH05_14305"
     CDS             complement(4000..4830)
                     /locus_tag="VFH05_14305"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6679444.1"
                     /translation="MDDLRNKDNSQAPSGTDTASDELADKVSAQLTGDQTLTMELPGE
                     QAEPVMLEEEKVKDEIDIQIDLLKDPDWVVRREAAITLGEMGDERCVEPLCKALYDGD
                     WQVREVAVEALGQIGSPAVEMLVKMVRDWDVRKYVLMALGKIRDERVLDPLMLQLRSD
                     EFKDDATNALVELGEPALERLVKALKDKDEMVRKQAVLALGRIKHGGAVDPLIEMLSD
                     ADWFTRLTAAAALEAIGDPRGREAIKPLTKDPDMVVRMRVERILAKWKKQPAGSASLN
                     "
     gene            complement(4888..5106)
                     /locus_tag="VFH05_14310"
     CDS             complement(4888..5106)
                     /locus_tag="VFH05_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679445.1"
                     /translation="MPKKHTTESDEVRLKKKIAERVAKPSHPEGDAAMRSLRKRLKRE
                     QRKRRAVAMRKKHAGGAKQAEAPAPAAG"
     gene            complement(5224..5850)
                     /locus_tag="VFH05_14315"
     CDS             complement(5224..5850)
                     /locus_tag="VFH05_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET6679446.1"
                     /translation="MTNHTSHIRQAILWTVTLTGMLFGACSWVPKGDAQFDVGIKDRG
                     VASWYGEQFHGKQAANGEIFNMEALTAAHRTMPLGSVVRVVNLSNGRHIHVRITDRGP
                     YVNGRILDLSHAAAVQLGMEHGGLTYVQVEIVGQRHPELVQLSEDSRRSLLPIDVTRV
                     PARPDSSLAPFLRVLPSDLLLTRRVRRIPGMLAADHTAHAQIATLELS"
     gene            6665..7663
                     /locus_tag="VFH05_14320"
     CDS             6665..7663
                     /locus_tag="VFH05_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6679447.1"
                     /translation="MIDVHHITKRYAQHTAIDRVTFSVAKGEVLAFLGPNGAGKTTTM
                     RILTCFMPATEGTAMVAGFDCADQPLEVKRRIGYLPETPPVYQELTVDEYLRFVGRLR
                     GLSGAMLTGAMAREVERLGLSAVQHRLIGNLSRGYRQRVGLAQALLHDPPVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSHSVILSTHILPEATAVCQRVVIINGGRIVAEDTPDRL
                     SARLRHSEKIALTLSRAPGEVEARLRQVTGVQHVHANGPPGHFLLESELGHDIREDVA
                     RLAVTNNWGLLELKSISMTLEDVFLKLTRHEEGVAQPAAGASDRVVVTEDKTESPS"
     gene            7660..8427
                     /locus_tag="VFH05_14325"
     CDS             7660..8427
                     /locus_tag="VFH05_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6679448.1"
                     /translation="MTPVNTILLKELRSHFVSPVVYVVGAVFLLIVGLLAYLYIVFTG
                     AQAIQLMQVQGQAQINLNDLVFRNLFSSIRFVLLIILPILTMRLFAEEKKLRTFELLM
                     TSPIGLNEIVAGKFMSVFLLFMGLLGLTGLIPLTLWLFSDFDWYPVLTGYLGLALLGA
                     LFLSVGVLASALTENQVVAAFVSFGLLLLLWLLAGLGSLLGDSTIGQVVSYLSFMEHY
                     DHLVRGLIDTQDLVYFATALILMLFLTHRVIDSTRWK"
     gene            8424..9947
                     /locus_tag="VFH05_14330"
     CDS             8424..9947
                     /locus_tag="VFH05_14330"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET6679449.1"
                     /translation="MNLKAIPFGVIGSVLALGGLTAYHFSPEQLWLVTAVESLALVCL
                     ILFLVVHRETFKTFSSRRSTRMGANSILMAVLFLGILGIVNFLAARHSIRWDFSENQN
                     FSLAPQTHRVIRSLPRDVKITVFTREKDPGYQSYKERLDSYRQASSKIAVEFVDPERQ
                     PKMAQSYGITRSDTAIFESGLQTVRVTNPSEAELTGALIRVSKDDKKRVMFLEGHGEP
                     GTENRDRTGFSLAKEILIKQGYEIGILSLLTQATVPDQTAILAIVGPRKPLTSDELNR
                     IHDYVEKGGRLLLMVDPDTQADFTALLSQWGVGLGRGVLVDLQDRLAQGDLTALLVRT
                     FTEHEITQDINAAVLFPLARHITFNDQIGKDWDFVPLARTSPNSWAETDLKGRVVSLN
                     EKEDIKGPLPMAAALTPKTAPAEGKTKSAMVVIGNSTFASNAFVNFPGNSDFFLHTVA
                     WLAQDRDMISIGPRDQVFRPFVPNPLQERTLLYVQVIFLPALVLIAGVQVWRKRRRL"
     gene            9974..11317
                     /locus_tag="VFH05_14335"
     CDS             9974..11317
                     /locus_tag="VFH05_14335"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET6679450.1"
                     /translation="MALVLSVLGLYLYLIEFPAKESQEREAATKNKLLTVDEHALTSL
                     TFKTGREELVFERTPDKGWMLIKPIRTETDTREISGLIRAIALGSVHRVVETNPTSLT
                     PFGLDTPVTTLEIQAGSMADTFLIGDTGPLTSTLYVLRNSDHAVLLTDLAPKDFVNKS
                     LMTFRRKDLLHLTSGNVDRIRLTYPTTEIVLYRVSDKPQPKWKIRYPVEAEADINEVR
                     MLLFRLEDLKAMGIVDPGPARDTLARTLKQPKVKITVHGEDGDQTVKLYQPNPSSGEA
                     IAETTPDAPLFRVNPTFVKEFTKDLYALQDKRLLGADERDIAMLSVKTRSERYTLINQ
                     NGEWVLEDRPTEKLNQPVANLFVSRVANVPAEERVLKQAGPLAPYGLVNPVAEFIATG
                     RDGKVTGKLLVGDQANGLAFATGHRLPGIYQIRADLLKQIPPTSELLAGPGKSSG"
     gene            complement(11318..11815)
                     /locus_tag="VFH05_14340"
     CDS             complement(11318..11815)
                     /locus_tag="VFH05_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679451.1"
                     /translation="MTSFGYMSGLGVVVLMFTLLPVNNAWSLDNPEKVIEETCAREWS
                     HNSRMRAACIEQQNSVLEKSRSTPVDPRLQHEDLSLIQERCEKNWPDDVRMRLQCQQQ
                     EMRAFQKLQGPPPKGISLQDYSVAVAQCSKDFPDDYRLRARCMDEQIAEKRRHQEPID
                     RSTTQ"
     gene            11907..11983
                     /locus_tag="VFH05_14345"
     tRNA            11907..11983
                     /locus_tag="VFH05_14345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11941..11943,aa:Arg,seq:cct)
     gene            12722..12967
                     /locus_tag="VFH05_14350"
     CDS             12722..12967
                     /locus_tag="VFH05_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679452.1"
                     /translation="MTCRRCQGCMARDHFLDLQESGGEWWLQGWRCINCGHVFDPVLE
                     KNRQLHAAAMASAEAQQSVTSEPIAVHGQEESLDVAA"
     gene            complement(13638..13922)
                     /locus_tag="VFH05_14355"
     CDS             complement(13638..13922)
                     /locus_tag="VFH05_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679453.1"
                     /translation="MFFAIGRWSASGGVAFDTETDGEGELAVEGVDGCDGATSCVVEY
                     LTGSDGGRAGGEGEGGGTAALGAGTIGIGFVSCVRNNDEMGSMGTRGGID"
     gene            13810..14352
                     /locus_tag="VFH05_14360"
     CDS             13810..14352
                     /locus_tag="VFH05_14360"
                     /inference="COORDINATES: protein motif:HMM:NF037406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679454.1"
                     /translation="MAPSQPSTPSTASSPSPSVSVSNATPPEADHLPIAKNICQRFHF
                     VARQLRLRGEYRSSVSVEDESDVQDLLHALLRVQFDDIGTDEWTPEYTNGASRTTFLL
                     NHDRLAVVAKKTRAGLTMTDLKDQVRTDIERYRARGRCTSLLCFIYDPDGRIGNPRGI
                     ESELTSTSEQFTVDIVVAPK"
     gene            14360..14773
                     /locus_tag="VFH05_14365"
     CDS             14360..14773
                     /locus_tag="VFH05_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET6679455.1"
                     /translation="MKRPKPVSAGPYQRPGPASATLTGASGIRYVGKEPAIPAVPCTI
                     HAPVDGGVVMADRAYILIHVLPGQTSRVVRALGEIKQIKTIDPCWGKPDIIAVVEVPD
                     QDALTQLVLSRIHTIDGVSQTDTHLVYRLKDTKSK"
     gene            14770..15216
                     /locus_tag="VFH05_14370"
     CDS             14770..15216
                     /locus_tag="VFH05_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679456.1"
                     /translation="MTLRSTPLLVALSLLTGVACAGPPIPHEPDVIDVTLSASSTDVR
                     MAVIQVLTDGGYEVEREDDQNLTTSYRKEISGPWDGLLRWRFGTGRSRIDAFVTPTDE
                     HTTRLRLHVQYDGKDGIFTRWEDSSTPLPQSAENQLRLIKNALHIL"
     gene            complement(15220..16629)
                     /locus_tag="VFH05_14375"
     CDS             complement(15220..16629)
                     /locus_tag="VFH05_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET6679457.1"
                     /translation="MQAAKILVVDDDAVARELLAEALKKEGYDVEAFGSGEAAIARGR
                     EGRVDLVLTDIRMGAVDGLTVLREFKRMSQDTAIVVLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKKEEIKLVVQRSLDHCRLLRENTKFREELKSKDEWSPLVGSSPAMLEVYKLVAR
                     VSESKSTVLLQGESGTGKELIARAIHGNSPRRDKPFVPVNCGALPDTLLESEMFGYEK
                     GAFTGAVGTKTGLFESASGGTLFLDEVGELGQALQVKLLRVMQDHEVRRVGGTHSIKV
                     DVRIIAATNRDLEQFVKEGKFRDDLFYRLNVVRITLPSLTERQEDIPMLAHYFLQKCA
                     TGTTTPVRGFHPDTMELLNRYRWPGNVRELENAIERAVSLSHGPLLTPDDLPVAIRQA
                     PIHADAKPESPDTTEPVCLTLEEVEKRHLVRVLKETKGNKVKASKILGIDRRTLYRMA
                     ERFGLDLGDEGESTEKETG"
     gene            complement(16638..17795)
                     /locus_tag="VFH05_14380"
     CDS             complement(16638..17795)
                     /locus_tag="VFH05_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6679458.1"
                     /translation="MPKQRNWVILTIAAAVFVVIVVLETVAPANIVGAYAFVLPILLV
                     ATLHSRPLMLATVAACVAATYLGLLQPTKPGRFQAAVINRTVVAGVLLIVAYVLMTRE
                     ERKVREETARQALAHQTESLLRANALLVDAKDQLNRSERLAAVGQLVASVAHEVGTPL
                     HSIAWHVQALGEEPSVTPDMKKRITVIDEQLNRVVRIIQDLLSSTRQRKPDPKWMSVD
                     EVITPATVLMEPAFHAKGVTLTSEMAIGVPSVWADAERLHQVLVNLLTNALAATGQHG
                     TVTVHAGARAATTEELEAGQRVAPDMSPMVVTVAVRDTGVGMPQSDVQRAFQPFFTTK
                     EVGKGTGLGLFLSRETVQAHGGTLSLMSEQGRGTTVTISLPGKVFVPEGLA"
     gene            complement(17953..20196)
                     /gene="malQ"
                     /locus_tag="VFH05_14385"
     CDS             complement(17953..20196)
                     /gene="malQ"
                     /locus_tag="VFH05_14385"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET6679459.1"
                     /translation="MDDTEQTLLAGLAERVGIAAEYHDISGVRHVTPDHTKRDILRAM
                     GFQVDSIATLAVAVREWDEAPWRRPCDPVRILHDEEVGAPVACFFQLEDGKERSVVVK
                     WELKDESGELLQQGQAGPGLAATEVKFIDGARRARVELPAPKGLSLGYYDLIIRAEGL
                     VGGSTGTLRLIVAPRQCYVPTTLEVGQRYWGLALQLYSLSSGRNWGCGDFTDLNKIVE
                     WAGKLGAALIGLNPLHAIRNTAPHHTSPYAPYSRLAINELYIDLERLPEFFASEEAQQ
                     QYRAPEFQAKLQALRENRRVDYDAIAAAKRTMLDLAYRQFLKEAYTGVEPNLEPQTAR
                     AKLLERFIQAEGEALELYAIFQALEEERRLIQSKHVSWQEWPKQFLSPGSAVREYGKR
                     HRKRIRFFQYIQWIASEQLRDVQRTAEAAGMPIGVYNDLALGAERTGAEAWMYQSVLA
                     LNAECGAPPDAFAPEGQNWGLPPINPVALRPSRYELLIQLLRRNMRFGGAIRLDHVMA
                     LCRLFWIPKGKAAADGTYVHYPFEDLLAIIALESVRSKTIVIGEDLGTVPDWVRDQLS
                     KAGVLSYRVFYFERGGDGSWKAPNEYPQQALAVVTTHDLPTLTGFWSGEDIQLRAGLG
                     AFGDEAGRRQAWDERQRDKGRMLAALKQEGLLPDGVTDDPAAVPAMTPALCRAIHTYL
                     SRTPSWLVLANIEDGLEEISQTNLPGTVENHPNWRRKYASSIENLVSDERLRELGATL
                     RSTRPIS"
     gene            20450..21178
                     /locus_tag="VFH05_14390"
     CDS             20450..21178
                     /locus_tag="VFH05_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679460.1"
                     /translation="MSAHRMIGHHLCVPAIALLALTAQAAEPTSTRLEIWGDYRYKIQ
                     ASEQPADAKDLACREALRQAVTNSTLYREHTASIVDHLLLRDIAWTLATRHVQDQEIL
                     EQGEQGRTVFCRVKAYLPADESARAIRVQLAGGQVPSEGLDQNRALRLLSVKEESPGT
                     LVIQYQALKRLDWLNTHYQGGLRDSADIMVDFYDAQGLLLVNRRYEARRTITGEDVMS
                     PGAVGVLQVPKPAAAKTYRVWLVK"
     gene            21490..22725
                     /locus_tag="VFH05_14395"
     CDS             21490..22725
                     /locus_tag="VFH05_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6679461.1"
                     /translation="MIFFGLAYFAHGIAGGLAKQPFTNYFKSLGMTADMVAAWLSLAA
                     IPWMIKPLYGLLIDLVPLWGYRRKSYLVLMAGCAAAGYAALAHVVSAEFVIWALLIST
                     LGIAAIDVVVDTLMVEEGLALGVIRRFQGQQWTWLNIAAITAGLLGGWLSHSLLPEAA
                     IRTAALIMIGGPVGVIIATWLFVEEPQRSFSRLHARTTAHEIAKGLKSKAFWLVGGFL
                     AFWNLIPNFSTPLYYHMVDRLGFDQYFIGQLASIGSVGATLGSFAYRSLSDRFSIHQT
                     LVVSILLSVIMALGYLLLQDGVSAAVLSFCSGIVSMLALLSLFTLAASICPPHAAGFA
                     FATLMSIYSATAQIGAMMGGYLYEWVFQQHIVPLIYLAALGTLSALAWIPFFPARPES
                     VRDEPSDEARHSTLRGLPA"
     gene            complement(22801..23406)
                     /locus_tag="VFH05_14400"
     CDS             complement(22801..23406)
                     /locus_tag="VFH05_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679462.1"
                     /translation="MNYGQRLVGIALLSVTITGCTSDIAKPVGLDVALRGVEEDMKAA
                     SGVSLHDVLSRDAIQEDEFNRAILQAQCFYRRPNPLVPVMIKDFTLTLQGTFTSQGKF
                     VVFGLSVPTGGIEIGTAKALQQTVELPVNFTSISSLPDVYLQQKGGYVKDFPESEKIK
                     YVEEVVKDRDILRAKIKELIASYSEQRCRQLLANTPLVGRP"
BASE COUNT         5119 a         7075 c         6506 g         5251 t
ORIGIN      
        1 gatttcaccc gacaaggaga ctgcgtgaat accacaagca tcgatcctgt gcgcacacat
       61 atcccgacca gtctggcacg attgcctgaa ctgtcacaga atttatggtg gagttggact
      121 gtggaagcac gccaactctt cgagaccatt gatcccacac tctggtttta tacccaccac
      181 aaccccgtca agttgctcgc cgacatccaa ccggagcgct tggcgaagct ggccgaagat
      241 ccttcgtatc tcagacaata ctccgccgtg ctgaaattgt tcgatgaata caggtcaaat
      301 ggccgcagct ggttcaccat gcagcatgcc agcgaaacca agaaagtcat tgcctatttt
      361 tcggcggaat ttggcctgca tacgtccatc ccgatttaca gcgggggctt gggcattctg
      421 gcgggagatc attgcaaaga agccagcgat ttgggcgttc ccctcgtggg catcggcttt
      481 atgtatcctc aaggctattt ccgccaacgg atcacagccg agggttggca ggaagccgcc
      541 tatgctccgt ttaaccgtga agactctcca attcatccgg cgttgacacc ttccggcgag
      601 gcttgccgct tcaccgtcga tatgggtggc cgtaatgtga cggcggtcgt gtggaaagtg
      661 ttggtgggtc gtatccccct ctatttgatc gataccgatg tgcccgagaa cagcgcggag
      721 aaccgggcgc tctccgctcg tctatacgga ggcgatcagg agatgcggct gtgccaagag
      781 atcctgttgg gtatcggcgg cgtccgtctt ctgcgtgcgc ttgatattgc gccttccgtg
      841 tggcatgcga acgaaggcca ttccgcattc ctcacgttag agcgtctgag ggaactcgtt
      901 cagaaaggcc tctcccatgc cgaagccagc gacatggtgc gacagagcac cgtatttacc
      961 actcatacac ctgttcccgc cggacatgac gtgtttcccc attacttgat ggatcggtat
     1021 tttgccggat attgggagca actgaacctt tcacgggatg aattcctggg cctgggggaa
     1081 acgccggaat cacgaggaca tggtttcaac atgacggccc tggtgatgcg cctctcagcc
     1141 catgtcaacg gggtcagccg tgagcatggc cgcgtctctc gcgagatgtg gcagcatttt
     1201 tggccgggac tgccggtcga agaggtaccc atccgtagtg tgacgaacgg catccatgca
     1261 cccacgtgga tcgcgcagga ccttcaccgc ctgtacagca agtctcttca cccggcctgg
     1321 tcggatcatt gcgacgaccc ggcaatgtgg cagcgcgtcc tcgatatccc ggaccatgaa
     1381 ctgtgggccg ttcgccaaac gatgaagcgg aagctcatgg gatttattcg tgagcgcgcg
     1441 cgcaccggat ggatgcaagg aaatcttcaa ccctcgcagg tgttgagccg aggcacgctc
     1501 ctcgatcccg aagccctgac gatcggcttt gcgaggcgat tcgccacgta caagcgggca
     1561 acgctcctct ttcaggatct cgaccgtttg aaacggctct tgcaagacaa atggcgcccg
     1621 gttcagctgg tgttcgcagg taaagcccac cccgccgatg agccaggccg atatttcatc
     1681 cacgaggtca tgaacttttg ccacgaccat aagctagggg gacatgtggc gtttcttgaa
     1741 gattacgaaa tgcatatggc caaatacttg gtccaagggg tggacatttg gctcaataca
     1801 ccccggtttc ccatggaggc gagcggcacg agcggaatga aagccgcctt aaacggggtc
     1861 cttcatttca gcacactcga cgggtggtgg acggaagggt acaacggggc gaacggctgg
     1921 gggattcagc cgttgcaggg gagccaggac ctccaagctc aggattccca cgatgccgaa
     1981 cagctgtacc gccagctgga gcaggaagtc gtccctctgt tctatcaacg tgacctcgat
     2041 ggaattcctc gcggctggtt acagatggtc aaggagagca tcagaaccat cgcgccgcaa
     2101 ttctgcacca agcggatggt caaagattac gtggagacgc tgtattgccc cgcgatgact
     2161 cgcacccctt caagctggtg aggacgcttc gctgatctat ctcatctgca tgctgcttgc
     2221 ctttgccgtg ccacccgacg tcgttgcctc caccgcgaca tcttcggaac gagaagccaa
     2281 ggacgcattc cgccgggctg actacgggac cgtcctgaag ctcctgcccc ccgcatcctc
     2341 cggaataacg ccttcgaaag catggcttcg gctggccgtc gacagcgcaa tacatgtcgg
     2401 tcgtccggaa gaggggttgg aaatctacga ccgcttggtc aagccggggc agccggaaga
     2461 ggtccccctc ctccagcggc tcggtatcgg tttcttacgg gcctacgtcc gcgattcgag
     2521 ggaacatctc cgcattgccg catacagcgc gttggctgaa ctcaatctcc cggaccagca
     2581 acccgtttta gaggacggct tgttggacgc ctccccgatt gtgcgggcga gggccatcga
     2641 agcgctcggc cgcgcgggac tcggtgcgcg ttcgcaagcg ttgcggcgcg caatgcagga
     2701 cgagattccg tcagtccgta tcgcggcgat gaacgtattg agtgaagccc agatcacgga
     2761 gatcatccct catgtagaaa aagtggctcg aactgaagac ggcccggaag ggatattcgc
     2821 ctatgccgcc ttgtatcgac tcggcaatcg agacatgctg gtcgacatta ccggcgcagc
     2881 cacgttacct gaccgggaga cacgtatggc cgcactcggt gtacttggcc ggttgaagca
     2941 ctcctcgagc ctggccgtcg tgagccaagg agtctatgat ccggaaccgc aagtgcgcgc
     3001 atttgcagcg ggagccttgg gtgaacttgg ccaacccggt gccgtcgctc ctctcaccca
     3061 tgcgctcggc gatgagaacc cgcgcgtacg cggagcggcc gcagcaagcc tcggccgcct
     3121 gggcgtgagc gatgcccgtc cgctgttgca tgctctggtt cgagacccgt ccatgcaagt
     3181 gagagccagt gccgtcgaag gcctcttgcg attgggcgat cagaccgcag tcttggtcgc
     3241 cacagacctg gcgcggcatc ctgatccctc agtcagggcc gctgctgccc aagcgctggg
     3301 cgctggatcc gacaggcaag cattggccgt acttcaaacg ttgttgcagg atcagcagcc
     3361 tcaaccacgg ctttttgcag ccaaagcctt gggtaaagtc tcattgcccg ttcttccgct
     3421 catcaaaaaa gcactgcatg actcggacgc ggcggttcgc attacctcgg cgggcagcct
     3481 gcttcgccaa cttgaacccg tcgccaagtc ctcgccacgt cgaggacgga aaggatagtc
     3541 atgtggagat ttgtcggcgc gctggtgatc gcctttgccg catttggctt gggatactac
     3601 ttcggacagc gaccgggagg caccttggaa caaacggtcg gtgatcttca gcattcattt
     3661 aaggatatgt cgaggaatgt cgtcgatacc accatgggca ttgagcggga tctccggaga
     3721 aggcaagcgc tgttggacac caagtcacgc gtggtgcagg cgaaatccga gctcttcgat
     3781 aagaacttcg gggaagcggc gaagcagctc gcggaagggg tggatgccct ggaaagttcc
     3841 gtaaaaggcg ccaagccgaa tgaacagact caggccgtac gaggattgat tggaacacta
     3901 caggaagctc ggctggaatt gacgatggga aaaccggtac cgattcgcaa actcaccgag
     3961 ttacagcaaa gcatcgatcg ggaactgaac aggtagccgt cagttcagag aggcggatcc
     4021 ggccggttgc tttttccatt ttgccaggat gcgctccact cgcatgcgga cgaccatgtc
     4081 gggatctttg gtcagtggct tgatcgcttc gcgtccacgt ggatcaccga tggcttcgag
     4141 agccgctgcc gcagtaagcc gcgtgaacca atcggcatcg gaaagcatct cgatcaaggg
     4201 atcaacggct ccgccgtgtt tgatgcgacc gagagccagg acggcctgct ttcgcaccat
     4261 ttcatccttg tctttgagcg ccttgaccag ccgttccaac gccggctcac ccaattcgac
     4321 caaggcattc gtcgcatcgt ccttgaattc atcgctccgc aattgaagca tcaggggatc
     4381 aagaacccgt tcgtcccgga tcttgcccaa ggccatcagg acgtattttc tgacgtccca
     4441 atcacgaacc atcttgacca gcatctctac ggccggagat ccaatctgac ccaatgcctc
     4501 gaccgcgact tcgcggactt gccaatcacc gtcgtagagc gccttgcaga ggggctccac
     4561 gcaacgctcg tcccccattt cacccagcgt aatggccgcc tcacggcgca cgacccagtc
     4621 gggatccttc aagaggtcga tctgaatatc gatctcgtct ttgaccttct cctcttcgag
     4681 catgaccggt tcggcctgct cgccagggag ctccatcgtc agggtttgat cgccggtcaa
     4741 ctgagctgag accttatcgg ccaactcgtc gcttgccgtg tcggttccgc tcggtgcttg
     4801 ggaattgtcc ttgtttcgca gatcatccat aatcaggatt ccttcaacag agacagcttc
     4861 gaggctgtga taatcccagc tctcttgtta tccagctgcc ggagcgggcg cttcggcctg
     4921 cttggcaccg cccgcatgct tctttctcat ggctaccgca cgccgttttc tttgctcgcg
     4981 cttgagtcgc ttgcgcaatg accgcatagc ggcgtcgcct tcgggatgtg atggtttggc
     5041 cactcgctcg gcgatcttct ttttcagtct gacttcgtct gactctgttg tgtgtttttt
     5101 gggcatcacc ccaccgcctc cttgattgtc ctcatcagct gacccaccag ggagccagtg
     5161 agaagaatca gtctagtgga gcgcttcgca tactgtcaac ccgatgtggg agcgaagagg
     5221 tgcctagctt aactcaaggg ttgcaatttg cgcatgagcc gtatgatccg cggccagcat
     5281 gccaggaatt cggcggacac ggcgggtgag caacaagtcg ctggggagaa cacggagaaa
     5341 cggtgctaaa gacgagtcgg gcctcgccgg caccctcgtc acgtcgatgg ggagcaatga
     5401 cctccttgag tcctctgaca gttgcacgag ctcgggatga cgttggccga cgatttccac
     5461 ttgaacgtag gtcagccccc cgtgctccat tcccaactgg acggctgcgg catgggagag
     5521 atcaagaatc cggccattca cgtacggtcc gcgatcggtg atacgcacgt gaatatgcct
     5581 tccattggac aggttcacga cgcgtacgac gctgccaagt ggcatagtcc ggtgggcggc
     5641 agtcagcgct tccatgttga agatttcgcc gtttgctgcc tgcttcccat gaaactgttc
     5701 cccgtaccag gatgcgacac cacgatcttt gatgccgaca tcgaattggg catcaccttt
     5761 gggaacccaa gaacacgccc caaagagcat gcccgtgagg gtgacggtcc agaggatagc
     5821 ctgccttatg tgcgaagtat ggttcgtcat gttcaacacc tgtgtgactc ggttgatctg
     5881 acaaggccaa gaaaacctcg cttgtggtgt tcaagctaag cctcaaccga gcaaccaaca
     5941 ataccgcggg ggtaccattg ccccctacct tggtgtgaag gcaaacagcc cacgcaaagg
     6001 caaaacagca cgcctcagca gacctcctaa cgctttgctc ctattagacc tgacattgta
     6061 tccatgaaaa cccgctcagg gttccaacaa ggctgacctt gcattgacta ggtgccaagc
     6121 cgaatcacct ctgcgaggac tcccccgggc gctttaacgt aggttaacca gactatgtca
     6181 ccctctttaa tggtgttgag cgacactctc cgattccccc tgattatctt ggtctgtgcg
     6241 gtcactgctg ctccaacggt catatggttt ttgggaccta agggggtttt caccacaata
     6301 aggggcggag tttgcgttaa ggtgatcgca ataaccgtcc cgtgcacctt atacgtgtta
     6361 tgtgacctcg cctctccaac ggcggctgag aggcaagaga ccaaagaaat cacgccaacg
     6421 gtaaaccaat aatacgtgct catgacgtcg acggaaggga aagcggtatc cggacgggcg
     6481 ggactctaac aggacccggt tgcttctgta aaggttgtcg gtgttggcgg caggcccatg
     6541 ttgactgccc ggcgtctcct gagtaagatg cccagcaagt gaattccagc tatggtgatc
     6601 ggacgcccac ctcagtaatc cctaaggcag accccatcca ccaccgagcc ccagattcgt
     6661 tggcatgatc gacgttcacc atatcactaa gcgttacgcc caacacacgg ccatcgatcg
     6721 tgtcacattt tccgtcgcta aaggcgaggt gctcgctttt ttaggcccaa acggcgcagg
     6781 aaaaacgacc acgatgcgga tcctgacctg tttcatgccg gcgaccgaag gcacagccat
     6841 ggtagcaggc ttcgattgcg ccgatcagcc ccttgaggtc aagcgccgga tcgggtacct
     6901 gcccgaaaca ccccccgtct atcaggagtt gacagtcgat gagtacctgc ggttcgttgg
     6961 acggctgagg ggattatccg gtgccatgct gaccggcgcc atggcccgcg aagtggagcg
     7021 gttgggcttg agcgccgtgc aacatcggtt gatcggaaac ctctcacgag ggtatcggca
     7081 gcgggtaggc ctggcgcaag cgcttcttca cgatccgccc gtgttgattt tggacgagcc
     7141 gaccgttgga ttggacccga agcagattat tgaaatccga gagctcatca agagtctggc
     7201 cggatcccat tctgtcattt tgagcacgca tattctccct gaggcaactg ccgtatgtca
     7261 gcgagtcgtc attatcaacg gaggccggat cgttgccgaa gatactccgg accggctctc
     7321 cgcacgactt cgccactcgg agaagattgc gctgacgctg tcccgagccc cgggcgaagt
     7381 cgaggcccgc ttgagacagg tcaccggcgt ccagcacgtg catgcaaacg gtccaccagg
     7441 acattttctc ttggaaagtg agttggggca cgatatcaga gaagatgtcg cccgcctcgc
     7501 cgtcaccaac aactggggtc tcctcgaact taaatcgatt tcgatgacgc tcgaagacgt
     7561 cttcctcaag ctgaccaggc acgaggaagg agtggcccag cccgcagctg gggcatcgga
     7621 ccgtgtcgtg gtcaccgagg acaagacgga atcaccctca tgacgcctgt aaacacgatc
     7681 ctgttaaagg agttacgctc gcacttcgtg tctccggtag tctatgtcgt gggagcggtg
     7741 tttttgctga tcgtcggact gctcgcgtac ctgtatatcg tcttcaccgg cgctcaagcc
     7801 atccaactga tgcaggtgca aggacaggca cagatcaatt taaacgatct ggtctttcgc
     7861 aacctgttca gcagcattcg gttcgtcttg ctgattatcc ttcccattct gaccatgcga
     7921 ctcttcgcgg aagagaagaa actccggacg ttcgaactgc tcatgacctc tccaatcggt
     7981 ctgaacgaaa tcgtcgccgg caagttcatg agtgtgtttc tgttgtttat ggggttactc
     8041 ggcctgacgg ggctgatccc gttaacctta tggttattca gcgatttcga ttggtacccc
     8101 gtgctgaccg gctacctcgg tctggcactg ctcggggcgc tctttctttc tgtcggtgtc
     8161 ctcgcttcgg ctttaactga gaaccaagtc gtggcagcgt tcgtgagctt cggcctcttg
     8221 ctcttgctgt ggctgctggc aggacttgga tctctccttg gtgacagcac gatagggcag
     8281 gtcgtctcat acctgtcgtt tatggaacat tacgaccact tggtccgagg actcattgac
     8341 acgcaggacc tggtgtattt tgccactgca ttgatcctca tgctcttcct cacacatcgt
     8401 gtcatagact ccaccaggtg gaaatgaacc tcaaggccat cccgtttgga gtgatcgggt
     8461 cagtgctcgc gcttggcggc ctaacggcct accacttttc accggaacaa ctctggctcg
     8521 tgacggctgt agagagtctc gcactcgtct gcttaatcct ctttttggtc gtacaccgtg
     8581 aaaccttcaa gactttctcg tcacgccgct caacccgaat gggcgccaac agcatattga
     8641 tggcggtgtt gttcctcggc atcctgggga tcgtgaattt ccttgccgcg cgtcactcga
     8701 ttcggtggga cttttcagaa aatcagaact tttcacttgc gccgcagacg caccgggtta
     8761 tccgaagcct gccgcgcgac gtcaagataa cagtctttac gcgtgaaaaa gatcccggct
     8821 atcagtcata caaagagcgt ttggacagct atcgccaggc gagttcaaag attgcggttg
     8881 aattcgtaga cccggaacgc cagcccaaaa tggcacagag ctatggcatc acgagaagcg
     8941 ataccgccat cttcgaaagc ggcctgcaga ccgttcgcgt cacgaatcct tcggaagccg
     9001 agctcaccgg tgcgcttatc cgagtgtcga aagacgacaa aaagcgggtc atgtttttgg
     9061 agggacacgg ggaacccggc accgaaaacc gcgatcgcac cggattttca ctcgccaagg
     9121 aaatcctgat caaacaaggc tacgaaatcg ggattctgag cttgctgact caagcgacgg
     9181 taccagatca aacagccatc ctggcgattg ttggaccgcg taaacccctg accagcgatg
     9241 aattgaatcg cattcacgat tatgtcgaaa aaggcggccg tctgctgttg atggtcgatc
     9301 ccgacaccca ggcggatttt actgccctgc tgagccagtg gggtgttgga ttgggccgtg
     9361 gcgtgctcgt tgatttgcag gaccggctgg cccaaggaga cctcaccgcc ctgctggtca
     9421 gaactttcac cgagcatgag attacgcaag acatcaatgc ggccgtgctg tttcctcttg
     9481 cccgtcacat cacgttcaat gatcagatcg ggaaagactg ggattttgtg ccgctcgccc
     9541 gcacgtcccc gaatagctgg gcggaaacag atttgaaggg ccgcgttgtg agcctgaatg
     9601 agaaagaaga tatcaagggt ccgctaccca tggccgctgc cctcactcca aaaacagctc
     9661 cggcggaagg caagaccaag tctgccatgg tggtcatcgg gaattcgacc ttcgcgagta
     9721 acgccttcgt gaacttcccg ggcaacagtg acttcttcct tcacaccgtc gcctggctgg
     9781 ctcaagaccg ggacatgatc agcatcggtc caagagacca ggtattccgg ccatttgttc
     9841 cgaatcccct tcaagagcgc acgctcctct atgtacaggt cattttcctg cccgccctgg
     9901 tgctcatcgc cggagttcag gtctggcgca aacgccgccg tctttaatga agcgctactg
     9961 gcctacagcc gtcatggctc tcgtgctttc cgtgcttggt ctctatctct atctcatcga
    10021 gtttcccgcc aaagagtccc aggaacgaga ggcggccacg aaaaacaaac tgttgacggt
    10081 cgatgagcac gctcttactt ccttgacgtt caagacgggc cgcgaggaac ttgtctttga
    10141 acggactccg gataaagggt ggatgctgat taaacccatt cgtactgaga cggacactcg
    10201 tgaaatcagc gggctgattc gtgcgatcgc gctgggatcg gttcatcgag tggtggaaac
    10261 caatccgact tcattgactc ccttcggact cgacacgccg gtcaccactc tggaaatcca
    10321 ggccgggagc atggcggaca cctttttgat cggtgacacg ggtccgctta cctctacctt
    10381 atatgtcctt cgcaattccg accatgcggt gctcctgacc gatctggccc caaaagattt
    10441 tgtgaataaa tcgctcatga ccttccgaag gaaagacctc ctccacctga catcaggaaa
    10501 cgtcgatcga atccgcctga cctatcccac aaccgaaata gtgctgtatc gagtcagtga
    10561 taaacctcaa cccaaatgga agattcgcta tcctgttgaa gcggaagctg acatcaacga
    10621 agtgagaatg ctgctctttc gactggaaga tctgaaagcc atgggcatcg tagaccccgg
    10681 cccggctcgc gatacgctgg ctcggacctt gaaacagccc aaagtcaaaa tcaccgttca
    10741 cggagaggat ggcgaccaaa ccgtcaagct ataccagccg aatccgtcaa gcggtgaagc
    10801 catcgcagaa accacgccgg atgcgccgct ttttcgggtg aacccaacct tcgtcaaaga
    10861 attcaccaag gatctctatg ccctgcaaga caagcgcctt ctcggcgcgg atgaacgcga
    10921 cattgccatg ctcagtgtga aaacacgctc agagcggtac acgctcatta atcagaatgg
    10981 ggaatgggtt ctggaagatc gtccgactga aaaacttaat caacccgtcg ccaatctgtt
    11041 tgtcagccgt gtggccaatg ttcctgcaga agaacgggtt ctcaaacaag ccggtcctct
    11101 ggcgccttat ggactcgtga acccggtggc ggaattcatc gcaacgggga gggatggaaa
    11161 agtgacgggc aaactgttgg tcggcgacca agccaacggt cttgcattcg cgaccggcca
    11221 ccggcttccg gggatctatc agatccgcgc ggaccttctc aagcaaatcc cccctacctc
    11281 tgaattatta gccggacctg ggaaatcgtc tggttgatca ttgagtcgtg ctccggtcaa
    11341 tcggttcctg atgcctgcgt ttctccgcga tctgctcgtc catgcaccgc gctcgcaaac
    11401 gataatcatc aggaaagtcc ttcgagcatt gcgcgaccgc aaccgagtag tcttgcaggc
    11461 tgatgccctt cggtggagga ccttgcaatt tctgaaatgc tcgcatttcc tgctgttggc
    11521 actgtagtcg cattcgtaca tcgtcaggcc aattcttttc acagcgttcc tggatgagcg
    11581 acagatcttc atgttgaagg cggggatcaa caggagtcga gcgggatttc tcgaggacgc
    11641 tattctgttg ctcgatacag gcggcccgca tacgagaatt atgagaccac tcgcgtgcgc
    11701 aggtctcctc gatcactttt tcaggattat cgagagacca cgcgttgttc accggcagga
    11761 gggtgaacat taagacaacg actccgagtc ccgacatgta cccaaagctc gtcatgccac
    11821 cacattaccc aaaaatcctc atatcgacaa cccgccgacg tcgttcccta gacacacttc
    11881 gcgtaagtgc gctaaattca agccacgccc ccgtagctca gttggataga gcagcggttt
    11941 cctaaaccgc gggtcgtacg ttcgattcgt atcgggggca ccaattccat cgtggaatac
    12001 cacctcccga gtccactggg tctggaggtc gacggtagcc gctgtcggct gtcttcctcg
    12061 tttcatagga acgtctccgt aggccaaatc gcacaaaaac tgcttagaaa tcatcttgct
    12121 atgactagag agcatggtag aatctttcta tggacgaacc ggctgtgaaa aaattcttta
    12181 cctcggtacg cgaacttcga gaagcgatgg cctctggcat gcatctcgat gattttgatc
    12241 ggctttgtct agaaaattat attgctttgc ttcagctaag ctatatcgat tggaaaaggc
    12301 aatattatcc aagtcatccc gacatccctc tctcagatcc cattgaaagc aacgacaatc
    12361 gcggatcctc tcgttcgcat taagcgctga ccatcctctt ctctgttcca cccgaagata
    12421 gttgttgcgt ctcgcagttc agcgtcactt ctaattgcca ctcgacatga ccgttccctc
    12481 ttgacctttc ctccctgccc ttccaaacac tgcggaaccg gcaccccgtg ccatgaactg
    12541 taatgaatac agcttattca aggagtcgta tttactgcct atcttgaaga acggcctgaa
    12601 tgaacagagg ggagccaaag agcccactga tacgcccacc tttcggagta ccgcctttgg
    12661 gtctcaacaa gagccgcctc acccgttagg cttgatgcgg gcgttaacaa aggagggtat
    12721 tatgacttgc cgacgttgtc agggttgcat ggcacgggac cacttcctgg atttacagga
    12781 aagtggaggc gaatggtggc ttcaaggctg gcgctgcatc aactgcggac atgtgtttga
    12841 tcccgtgctc gagaaaaacc ggcagcttca cgcggcggcc atggcttcgg cggaagctca
    12901 gcagtcagta acttcagaac caattgcggt ccacggacaa gaagaatctc tggacgtcgc
    12961 cgcctgagag gtgtttgtag tctgtcgccc gagtgcagtg gtccttgctg actttcttct
    13021 gttttgttgc ctgtctacag gacgtgggct acactttgca cacattttat ggcacgtgca
    13081 aaatccgatc gggcgcggtc gaagacatct gcgatcgatg acctgacgca aagcatcgag
    13141 caattgcaag cattccttcc gaccgttgaa gatcttggaa gggaagggtt tccttacctg
    13201 gagggcgcga aggccagaac tgaacttcag cttcgtgagt gcataaaacg cgcctttggg
    13261 gataaatccc aggaattcca agcgcatcgc caccataagt tcgccgaacc aaagcagacc
    13321 gctgccctca tccgaagtct cgtagcagcc ttggaggatc ggaagctcga gctgcaaggc
    13381 ctaaagcccg cgactccaga gccacccgaa cgttcgaccg agcccgctcg cccaacgatg
    13441 gcgctggtgc ctccatctac acctcctaca tctccagcac cagctccctc agtcacctct
    13501 tcaatgacat catcgccacc ggttttcctg catgtgacac gaaacacgac gacagagcag
    13561 tcagcggccg ttcctgcagc cagcacttcg cctactgccc ctgcgcccgc tgtcgaggtg
    13621 agtgcggact caccgtctca atcgatacca ccacgagtac ccatcgagcc catctcatcg
    13681 ttgtttcgaa cgcaggagac gaaaccgatg ccgatagttc ccgcaccaag cgcagctgtg
    13741 ccgcctcctt caccttcacc acctgcacgg ccaccatctg aaccggtcag gtattcgacg
    13801 acgcaagaag tggcaccgtc acagccatca acaccttcca cggcgagttc gccttcgcca
    13861 tccgtctcgg tatcgaacgc tacgccgcct gaagcggacc atctgccaat cgcgaaaaac
    13921 atctgccaac gctttcactt tgtcgcgcgg cagctacgct tgcgaggcga ataccgctcc
    13981 agcgtgagcg tcgaagatga atcagatgtc caggatctgc ttcacgccct tttgcgcgtg
    14041 cagtttgacg acatcggcac ggacgaatgg acgccagagt atacgaacgg agcctctcgg
    14101 acgacctttc ttctgaatca cgaccgtctg gcagtcgtcg cgaagaaaac acgcgccgga
    14161 ctcacgatga cggacttgaa agaccaggtg cggacggaca tcgagcggta tcgggctcgc
    14221 ggtcgatgca cctccctgct gtgttttatc tacgacccgg acggccgcat tgggaacccg
    14281 cgagggatag aaagcgaact gaccagcacc agtgaacagt ttaccgtcga cattgtcgtc
    14341 gcaccaaagt gaggcaggca tgaagagacc gaaacccgtt agcgccggtc cgtatcaacg
    14401 ccctggaccc gcttccgcca cattgacagg tgcttcaggt atccgctacg ttggcaaaga
    14461 gcctgccatt ccggcggtgc cttgtacgat ccatgcaccg gttgacggtg gagtggtcat
    14521 ggccgaccgt gcgtatattt tgattcacgt cttgccgggg caaaccagcc gtgtcgtgcg
    14581 agccctcgga gagattaagc agatcaaaac gattgatccg tgctggggga agccggatat
    14641 catcgccgtc gtcgaagtgc ccgaccaaga tgcattgacg caactggttc tgagccgtat
    14701 ccatacgatc gacggagtct cacaaacgga tacccatctt gtgtaccggt taaaggacac
    14761 gaagtccaag tgactttgcg cagcactccg ctcctggttg ccctctcgtt gctcaccggc
    14821 gtggcctgcg ccgggccgcc tatcccccat gaacccgacg tcatcgacgt cacgttgtct
    14881 gcatcgtcca cggacgttcg gatggcggtc atccaggtgc tgaccgacgg cggatacgag
    14941 gtggagcgag aggacgacca gaatctgaca acttcatacc ggaaagaaat cagtggaccc
    15001 tgggatgggt tattgcgctg gcgcttcggc acaggcagaa gtcgtatcga tgcttttgtc
    15061 acacccaccg acgagcacac aaccagactc agactgcacg ttcaatatga cggaaaggat
    15121 ggaatcttta cgcggtggga ggattcgtcg acgcccttgc ctcagagtgc ggagaatcaa
    15181 cttcggttga tcaagaacgc cctgcacatt ctctaatacc tacccagtct ccttctcggt
    15241 gctctcgcct tcatccccga gatccagtcc aaaacgttcc gccatgcgat aaagggtccg
    15301 tcggtcgata cccaagatct tggacgcttt gaccttgttc cctttggttt ccttgagcac
    15361 gcgtaccaaa tgccgcttct caacctcctc gagtgtaaga cacaccggct cggtcgtgtc
    15421 cggcgactcc ggcttggcgt ccgcatgaat cggagcctgt ctgattgcaa caggcaaatc
    15481 atccggcgtc aaaagcgggc cgtgactgag ggagacggcg cgttcgatcg cattctccaa
    15541 ctcgcgaacg ttgcccggcc atcgatatcg gttcaagagc tccatggtat ccggatgaaa
    15601 acctcgcacg ggagttgtcg tgccggtggc gcacttctgg agaaaataat gcgcgagcat
    15661 cggaatgtct tcctgtcgtt cggttaagga cggcaaggtg atccggacga cgttcaagcg
    15721 ataaaacagg tcgtcgcgaa atttcccttc cttaacgaac tgttccaagt cccgattggt
    15781 cgcggcaatg atccgcacgt ccactttgat ggaatgtgtc ccacccacac ggcgaacctc
    15841 atgatcctgc atgacgcgca acaacttgac ttgcagagcc tgtccgagct cgccaacctc
    15901 gtcgagaaac agcgtgccgc cgctggccga ctcgaacagg ccggtcttcg tcccgaccgc
    15961 ccctgtgaac gcgcccttct cgtagccaaa catttccgat tcgagcagcg tgtccggcaa
    16021 ggctccgcag ttcaccggga caaaaggctt gtcgcgtctc gggctgttcc cgtgaatggc
    16081 ccgtgcgatc aactctttcc ctgtcccgct ctctccttgc aacaacaccg tgctcttgct
    16141 ctcggacaca cgagcgacca gcttgtagac ttccagcatc gccgggctgc ttccaaccag
    16201 cggagaccat tcgtccttac tcttgagttc ttcacggaat tttgtattct cccgtaacag
    16261 gcggcagtgg tcgaggctgc gttgaacgac gagcttgatc tcctccttct taaagggctt
    16321 ggccagatag tcgtacgccc cttgcttgat cgcctcaatc gcaccctcca acgatccgaa
    16381 ggcggtcaag actacgatcg cggtgtcctg gctcatgcgc ttgaattctc gcagaacagt
    16441 caatccgtcc acggctccca tgcggatatc cgtaagaacc aaatcgaccc ggccttcccg
    16501 tcccctggca atggccgcct cgccgctgcc gaaggcttcg acatcgtacc cttctttctt
    16561 cagcgcctcg gccaaaagct cccgagccac ggcatcatca tcgacgacta agattttcgc
    16621 tgcctgcatg acctccccta ggccagccct tccggcacga agactttgcc gggcaggctg
    16681 atcgtgaccg ttgttcctcg tccctgttcg ctcatcagcg acaacgtccc gccatgagcc
    16741 tggacggttt cccggctcaa gaatagaccc aagcctgtcc ccttgccaac ctccttggtc
    16801 gtgaaaaacg gttggaacgc ccgctgaaca tcggattgtg gcatgcccac acccgtatcg
    16861 cgaacggcaa ccgtgacgac catcggcgac atgtcggggg cgacccgctg gccggcctct
    16921 agttcttccg tcgtagcggc acgtgcgccc gcatggacgg tgaccgtccc atgctgtccg
    16981 gttgcagcca gtgcgttggt gagcaagttg acgaggacct gatgcagcct ttccgcatca
    17041 gcccagacgg atggaacgcc gatggccatt tcgctcgtca acgtgacgcc tttcgcatgg
    17101 aaagccggct ccatcaacac ggtggccggc gtgatgacct cgtcaaccga catccatttc
    17161 gggtccggtt tccgttgcct cgtagacgag aggaggtcct ggatgatgcg caccacgcga
    17221 ttgagctgtt catcaatgac cgtgatccgc tttttcatgt ccggcgtcac gctgggttcc
    17281 tcgccaaggg cttggacgtg ccaggcgatg gaatgcaagg gcgttcccac ttcgtgggcc
    17341 actgatgcca ccaactgccc aactgccgcc aaccgttccg agcgattcaa ctggtccttg
    17401 gcatcgacca ataaggcgtt cgctcgcagc agactttccg tttgatgtgc caacgcctgg
    17461 cgcgctgttt cctcccgcac tttgcgctct tctcgagtca tcagcacgta cgcgacaatc
    17521 agcaagacgc cggccacgac cgttcggttg atgacggccg cctggaagcg gcccggcttc
    17581 gttggctgca gcagacccag gtacgtcgcg gcgacgcagg ccgcaacggt ggccagcatt
    17641 aagggacggc tatgaagcgt ggcgaccagc agaatcggca atacgaaggc ataggcgccg
    17701 acaatattcg ccggcgcgac ggtttccaac accacgatga cgacaaacac ggctgctgca
    17761 atggtaagaa tgacccaatt tctctgcttg ggcatgttgg aacctgaagg actcaagcca
    17821 cccacagacg acagcagagg ctccgcgata cggcttcact taccggctgt cggaacaggg
    17881 cctccaggcg ggcgtgcagt ttcgtgtctc gcacaaggct gccagttatt gcagagtccg
    17941 ttctgcagcg tcttagctga tcgggcgggt cgaacgcaag gtcgcgccaa gctctcggag
    18001 tcgttcatca ctgaccagat tttcaatgga gctggcgtac tttcgccgcc aattcggatg
    18061 gttttcgacc gttccaggca ggttcgtctg cgagatttcc tccaggccgt cctcgatgtt
    18121 cgcaagcaca agccatgacg gcgtgcgaga cagataggta tggattgccc ggcacaacgc
    18181 tggcgtcatg gcgggcaccg ccgccggatc atcggtgacg ccatcgggta acagtccctc
    18241 ctgcttgaga gcggccaaca tacgtccttt gtcgcgttgc cgttcgtccc aggcttgacg
    18301 acgaccagcc tcatcaccaa atgcgcccaa gcctgcgcgt agctgaatgt cctcgccgga
    18361 ccaaaaaccg gttaatgtag gtaaatcatg cgtcgtcacg acggcgagcg cctgctgcgg
    18421 atactcattc ggggccttcc aggatccatc gccaccccgc tcgaaataaa agactcgata
    18481 tgacagcaca ccggctttcg acaattggtc tctgacccaa tcgggaaccg tgccgagatc
    18541 ttccccgatg acgattgtct tagatcgaac actttccaaa gcaatgatcg ccaacaaatc
    18601 ctcgaacgga taatggacgt acgtgccgtc tgctgcagcc ttgcccttgg gaatccagaa
    18661 gagccggcac agcgccatca cgtgatcgag acggatggca cctccaaatc gcatattccg
    18721 tcggagcagt tgaatcagca gctcatagcg actcggccgt aatgccaccg gattgatcgg
    18781 cggcagcccc cagttctgcc cttcaggcgc aaacgcgtcc ggcggagcac cgcactcagc
    18841 attcagcgca agcacagatt ggtacatcca tgcctccgct cccgtgcgtt ccgcacccaa
    18901 cgccagatcg ttatagacac caatcggcat cccggcggcc tcggctgtgc gctggacgtc
    18961 acgaagttgt tctgaggcga tccactggat gtattggaaa aagcgaatcc gcttccgatg
    19021 gcgtttgccg tattctctta cggcggatcc cggcgacaaa aactgcttgg gccactcctg
    19081 ccaactgacg tgcttcgact ggatcaaccg ccgctcctct tcaagtgcct ggaagattgc
    19141 gtagagttcg agcgcctcgc cttcagcctg gataaatcgc tcgagcagtt tggcccgggc
    19201 cgtttggggc tcgagatttg gctctacgcc ggtgtaggcc tctttgagga actgccgata
    19261 agccagatcc aacatcgtgc gcttagccgc cgcgatcgca tcataatcca cgcgccggtt
    19321 ctcgcgcagc gcttggagct tggcttgaaa ctccggcgcg cgatactgct gttgcgcctc
    19381 ctccgaggcg aagaactccg gaagccgttc gaggtcgata tataattcgt taatcgccag
    19441 ccgactgtac ggcgcatacg gactcgtgtg gtgcggcgca gtgttgcgga tggcgtgcag
    19501 cgggttgagg ccgatgaggg cggcgcccaa ttttccagcc cattcgacaa tcttattcag
    19561 atccgtaaaa tctccacaac cccaattgcg tccggaggac agcgaataca attggagagc
    19621 aaggccccag taacgttgac ccacctccaa tgtcgtcggg acatagcatt gccgcggcgc
    19681 cacgatcagt ctgagtgtgc cggtcgaacc accgaccagg ccttcggcgc gaataatgag
    19741 atcgtagtat ccaagcgaca gacctttggg cgcgggtagt tcgactcgtg cacgccgggc
    19801 gccgtcaata aacttcactt cagtcgcggc caggcccggc ccggcttggc cttgctgcaa
    19861 caactcgccc gattcgtctt tcaactccca cttcacaacg accgagcgtt ccttcccgtc
    19921 ttcaagctgg aaaaaacagg caaccggtgc gcccacttcc tcgtcgtgca agatgcggac
    19981 aggatcgcag ggcctgcgcc acggtgcctc atcccattca cggaccgcaa cggcgagtgt
    20041 cgcaatcgaa tccacctgaa aacccatcgc tcgaaggatg tctcgcttgg tgtgatcggg
    20101 cgtgacgtga cggacaccgc tgatatcgtg atattccgcc gcaatgccga ctcgctcagc
    20161 gaggccagcg agcagcgttt gttccgtgtc atccataaat cgtgagtctg cgtgaagacg
    20221 aaacgcaagt caagctatcc tgttgttgtc ctgaatgaat tttatacacg catagtaaaa
    20281 tggtggaccg aatcaatggc cctgtactta agacaatgga ccgtcggcgc cgtccggaga
    20341 cagccaggtt tgtgacccgc gtcctgaaag tggaagccac aacgcgtgcg tggtagcata
    20401 accgccgggc ggtgaacaat ttgccacctg ttactgagga gggcggaaca tgtcagccca
    20461 tcgaatgatc ggtcatcatc tttgtgttcc ggcgattgca ctcctggcgc tcaccgcaca
    20521 ggcagcagaa ccaaccagca cccgtctcga aatctgggga gactatcgct acaagattca
    20581 agcctccgaa caacccgccg atgccaagga ccttgcctgc cgggaggcgc tccgccaggc
    20641 tgtcacgaac tcgacccttt accgggagca cacggcatcg atcgtcgatc acttgctgtt
    20701 gcgcgacatc gcttggacgc tcgccacacg tcacgtgcag gatcaagaga tcttggaaca
    20761 aggcgaacag ggacgaacgg tcttctgtcg agtcaaggcc tacctgccgg cagatgagag
    20821 cgcccgtgcc atccgggtgc aactggctgg agggcaggta ccgtcagaag gattagatca
    20881 aaaccgcgcg ttgcgactgc tcagcgtgaa agaggagagt cccggtacac tcgtgattca
    20941 gtatcaggcg ctcaaacgat tggattggct caatacccat tatcagggag gcctgcgcga
    21001 ctcagccgat atcatggtgg acttttatga cgcgcaaggc ctgctcttag tcaaccgtcg
    21061 ctacgaagca cggcgaacga tcaccggtga agacgtcatg agcccaggcg ccgtcggcgt
    21121 cctgcaggtt ccgaaaccag cggccgcgaa aacctaccgc gtgtggctgg tgaaatagct
    21181 cgcccccgta ctacaccgca gcgccgcacg atcgagcact caattccagt cctgcacacc
    21241 ttcaagacgt ggctcgctca taacaagcac cgaattcccc tccgacaggc ggccaattga
    21301 aagagtgtag cgtggatcca tccttgatca gctcgagttg gagccgacca tgtctcggtg
    21361 cggccctccc cttacccgca agggctctcc cgagtgttcc tccgaccgcg gccgagtgca
    21421 gtgtcacggg ttatgtggag aggaggaatt gatgagagca gaggcacgtc actagctgaa
    21481 cgccgtctaa tgattttttt tgggctggcc tatttcgcac atgggatcgc cggcgggctg
    21541 gcaaagcaac ccttcaccaa ctatttcaaa tcattgggaa tgaccgcaga catggtggcc
    21601 gcctggctgt cgttggcggc gattccatgg atgatcaagc cactgtacgg tctcctgatc
    21661 gacctcgttc cactgtgggg ttatcgaaga aaaagttacc tggttcttat ggcggggtgc
    21721 gcggctgcgg gctatgccgc actggcccac gtcgtctcag cggagtttgt catatgggcg
    21781 ctcttaatca gcacgcttgg tatcgcggcg atcgacgtgg tcgtcgacac gttaatggtc
    21841 gaagaaggtt tggcattggg tgtgatccgg cgattccaag gtcaacaatg gacgtggctc
    21901 aacatcgccg caatcacggc ggggttgctc ggtggatggc tcagtcattc gctgttgcct
    21961 gaagccgcca tccggacagc ggcgctcatc atgatcggcg gaccggtcgg agtcatcatc
    22021 gccacctggc tcttcgtcga agagccacaa cgatcctttt ctcgcttgca tgcgcgtaca
    22081 acagcccatg agatcgccaa aggcctgaag tcgaaggcgt tctggctcgt cggtggcttt
    22141 ttggccttct ggaatctgat tcccaacttt tcgacgccgc tctattacca tatggtcgat
    22201 cgacttggct tcgaccaata ttttattggg cagcttgcct cgatcgggtc cgtgggcgcg
    22261 acgcttggga gctttgccta ccgctcgttg tccgaccgct tttccattca tcagacgctg
    22321 gtcgtgagta ttcttctcag cgtgatcatg gctctcggct acctgctctt acaggatggc
    22381 gtaagcgccg ctgtcctctc cttttgctcg ggaatcgtga gcatgctggc gctgttaagc
    22441 ctgttcacgt tagccgcttc catctgcccg cctcatgcag ccggctttgc ctttgcgacc
    22501 ttaatgtcaa tctatagcgc aacggcgcaa atcggcgcga tgatgggagg atatttgtat
    22561 gagtgggtgt ttcagcaaca catcgttccg cttatctatc ttgccgcgct aggcaccctc
    22621 agcgcacttg cctggattcc gttcttcccc gcacgtcctg agtccgtgag agatgaaccg
    22681 tccgatgaag cgaggcattc taccctgaga ggcctgccgg cctgatgtag tatacgttct
    22741 tcctgtgctc gacagtctct tcatcatcct tgttgaggag gaggtcagta acggcctgga
    22801 tcacggtcgt cctaccaacg gggtattcgc aagcaactgt cgacaccgtt gttcggaata
    22861 gctcgcgatc aactccttga tcttagcgcg aaggatgtcg cgatccttta ccacttcctc
    22921 cacatatttg attttttcac tctcaggaaa gtccttcaca tagccgccct tctgctgaag
    22981 gtagacatct gggagcgatg agatcgaagt aaaattgacc ggaagctcga ccgtttgttg
    23041 tagtgccttt gcggtgccga tttcgattcc tccggttgga accgaaagac cgaacaccac
    23101 gaactttcct tggctggtga atgtgccctg caacgtcaac gtaaagtctt tgatcatcac
    23161 agggacaagc ggattcggcc tccggtaaaa gcactgcgct tgaagaatgg ccctgttgaa
    23221 ctcgtcctct tggatggcat ctctgctgag cacgtcgtga aggctcacgc ccgaagcggc
    23281 cttcatatcc tcctccacgc ctctaagcgc cacgtccaga cccaccggtt tggcgatgtc
    23341 gctcgtacat ccggttatcg tcacacttag caaggcgatg ccgaccaggc gctgcccata
    23401 gttcataaaa gacctcacgg ttatgtgccc ttgaaatgtc gaagaagtga tcgtccatct
    23461 gacagaatgg gcaaggtagg ccgtcggttg gtcgctgtca agcgcaggag gaatgggtat
    23521 gtagacgagc ggcttacttc tacgacgctg ctcccaagtt ttctcacaca gtcatgcatt
    23581 ctcaaaacga ggatggtgga acacttcgcg acgattgtgt ctctgtaaga ttccgtcctc
    23641 acttgttgag cattttctgt ctcgcactat catttttggg accttgtttc taggcatgcg
    23701 aacagggagc gcgctacatc catttgtaaa tccttgaatt cgcgtcagac gactttggcg
    23761 ttgcctacgg cccaaggatt gcttatcccc gacgcggctc ggcaccgatc agctgaaagg
    23821 aggtcaattg acagggcggg ctcatacacg ctagtgagct gaagtcagct tcgctttcac
    23881 acaaggtcct ggtttaccaa actcacaatt tcatactgaa tatggaggaa tcgaatgagg
    23941 gcgatttcga t
//
LOCUS       DASSCQ010000228         7612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_934222, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000228 DASSCQ010000000
VERSION     DASSCQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934222"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1940
                     /gene="pgm"
                     /locus_tag="VFH05_14405"
     CDS             297..1940
                     /gene="pgm"
                     /locus_tag="VFH05_14405"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HET6679463.1"
                     /translation="MGVHPLAGQPAPETMLVDVNKLLEAYQDVPDPAIPGERVSFGTS
                     GHRGSSLKRSFNEHHILAVVQAICEYRHRQGTTGPLFVGIDTHALSRPALDTTLEVLA
                     GNGIEVMVDRHGGYTPTPVISHAILTYNRGRSSGHSDGIVITPSHNPPEDGGIKYNPP
                     QGGPADTTATKWIEERANSLLAAKLQGVIRRAGSSSKRNPLVHEHDYIGAYVADLEHV
                     IDFEPIRASKLKLGVDPLGGSGVAYWEPLAERYRLNIEIVNASVDPTFRFMPLDWDGR
                     IRMDCSSPYAMANLIALKDRFDVAFGNDADNDRHGIVTGSAGLMNPNHYLAAAIAYLF
                     SHRPHWRAGARVGKTLVSSSLIDRVAQKLRRMLVEVPVGFKWFVDGLLDGSLGFGGEE
                     SAGASFLRRDGLVWTTDKDGIIMDLLAAEMMATTGRDPSHLYRTLTVELGEPVYARID
                     APATPQQKAALSKLSSDQVPAQTLAGDPILARLTTAPGNGAAIGGLKVVTAQGWFAAR
                     PSGTEDVYKLYAESFKGPDHLSLIQAEAQALIAKVLTPA"
     gene            complement(1937..2836)
                     /gene="lipA"
                     /locus_tag="VFH05_14410"
     CDS             complement(1937..2836)
                     /gene="lipA"
                     /locus_tag="VFH05_14410"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET6679464.1"
                     /translation="MTSDSPKSLISIETLRSHSRPRLPAWFRVEARTGSDYLDIKQTV
                     DRLKLHTICEEGRCPNRWECWNARTATFLILGDVCTRRCHYCSVSTGRPGAVDQDEPR
                     RIAEAVKALRLRHAVITSVNRDELADGGAAIFAETIRHTRRVNPDCTIEVLIPDLEGN
                     AAALDVIFEAQPDVLNHNIETVPRLFPILRPQGKYQRSLEVLARAKRARLTTKSGLII
                     GMGETPDEIRLVLHALRRAGCDIVTIGQYLQPTTQHLPVAHFYEPASFTAFKTEALAM
                     GFVHVESGPLVRSSYHADQHVSR"
     gene            complement(2829..3197)
                     /locus_tag="VFH05_14415"
     CDS             complement(2829..3197)
                     /locus_tag="VFH05_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679465.1"
                     /translation="MDEKKDAINSLVDRFQLAGLDDRQRGFSRPVDIDPVEGQYRARL
                     RYESTQIVTGPAATEAAALLVLIHDLHAQGYRQLKTQLSFRKGTYLGSQEPWIEYADP
                     APDRGSGLLSTIRNWFHGSD"
     gene            complement(3373..3738)
                     /locus_tag="VFH05_14420"
     CDS             complement(3373..3738)
                     /locus_tag="VFH05_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679466.1"
                     /translation="MPATAKDQIEIAAALVRLYVFLSQYLDRCVDDVARASYPDSELE
                     AHLTETRRRLLSILSVNPVVKKKLTEECDRILALGASCLKAGPGNAQLHERLAAERTV
                     LTQKTLALSDLVAVFRALD"
     gene            complement(3766..4071)
                     /locus_tag="VFH05_14425"
     CDS             complement(3766..4071)
                     /locus_tag="VFH05_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET6679467.1"
                     /translation="MGEFVRVAAVDEVKPGQGLVAEVNGKALAVFNVDGQIHVIDNTC
                     VHRGGPLGEGELEATVVTCPWHGWQFDVTTGACVNNPAARVQTYPVKVEGSDVKVQL"
     gene            complement(4102..4965)
                     /locus_tag="VFH05_14430"
     CDS             complement(4102..4965)
                     /locus_tag="VFH05_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET6679468.1"
                     /translation="MADEEQAAQPKENLIPGVKHVIAISSGKGGVGKSTVAANLACAL
                     ALTGAKVGLMDADLYGPNIPMMMGSTAGPEQRDGKIVPVENHRVKLISMAYLVPEEAP
                     LVWRGPMVHQYLQAFFRDVLWGDLDYLLIDLPPGTGDVQLSLSQMVPLAGAITVTTPQ
                     EVALYDVRKGMAMFQKVNVPLLGIIENMSFFVCGHCGERTDIFSHGGGERAASKLGIP
                     FLGSIPIDPVIREGGDTGHPIVVADPGSPQAAAFREIAKKLMESLAGAAKGGASIDSL
                     LKKIKQPFTNQ"
     gene            complement(5154..6005)
                     /locus_tag="VFH05_14435"
     CDS             complement(5154..6005)
                     /locus_tag="VFH05_14435"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6679469.1"
                     /translation="MPDAVGTTPFAYKTFTLFCLVVAVTLIGTPLFGYLYGYTLFDWA
                     LFAILYVCTGLGITVGYHRLISHRSFECHPWVKALLLIAGGWALQNSALRWSADHLRH
                     HARTDQEEDPYNAQKGFWHSHCIWLFINTSHRTENYERQLRKDPIVMWQHRHYHAVVI
                     SGLAFPLLLGYLHGGLIGGIGCFFLAGMFRMFMVLNSTFAINSLCHMFGTQPHGTGDS
                     SRDNWLVSFLSFGEGYHNYHHTYARDYRNGPRWYNFDPSKWTIYCLSMLGLATNLRRL
                     DSTVLSE"
     gene            6291..>7612
                     /locus_tag="VFH05_14440"
     CDS             6291..>7612
                     /locus_tag="VFH05_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET6679470.1"
                     /translation="MAAIDPLESSVQHIGGELARLSAGQAPALFERQWWSQAAINLAM
                     HDLDFKTQLFRFIDVLPSVADDQRVVTLAQEYFGAMADQAFGLRWGLKALSATSIGAR
                     LSGRAIRAQVEQMARTFIAGSSIEDAVPVLGALWASGKAWSVDLLGEATISHQEADRY
                     RDRCVAALDSFAQAARVWPAIPLLENDHLGAVPRVQLSLKISALSPFLDPIDPEASFE
                     SVASRLRPIVDMAMRAPASLIFDMEQADTKSLVIEIFTRLFTETPYRQFPHAGLALQA
                     YHRETTQDVASLLGWVRTRGVPITIRLVKGAYWDSDTIRYRQRGWPVPLFEEKAETDF
                     NYESLVRILLEASSLIRPAFGTHNLRTLAYVEAVAEQLGLPPSTWEYQMIFGMAEPFQ
                     QAVLRRGRRLRLYTPVGDLLPGMAYLVRRLLENTSNESFLRKEYVESQP"
BASE COUNT         1530 a         2224 c         2266 g         1592 t
ORIGIN      
        1 tggccgcctg tccgtgaagc cgcaagacgt gcactttgcg gttcacgcgc tcggcaatcg
       61 ccacaacgga ctcatagacg gcgctgccgg tcagcgataa ttccctttgg aggcggccaa
      121 gaaattcgag tgacataaaa agatcgaagg tctgataagg gatattatgt caacttgcgt
      181 tcatttctca atctaggcct ggacgcccgt acttactctc ttctccccca tccggtcaat
      241 cgaatcgacc gctttcgtac attgacaagc cggggctgca acgttacacc aagagcatgg
      301 gtgttcatcc tttagctggt cagcctgcac cggagacgat gttggtggac gtcaacaagt
      361 tgctggaggc ctatcaggac gtcccggatc cggcaatccc cggtgaacgg gtaagttttg
      421 ggacctcagg acatcgcggt tcatccctca agcgatcctt taacgaacat cacattctcg
      481 ccgtcgtcca agcgatttgc gaataccggc atcgtcaagg gaccaccggt cccttatttg
      541 tgggcatcga cacgcatgca ctctcgcggc ccgccttgga cacgacgctg gaggtgttgg
      601 cgggcaacgg tatcgaggtc atggtggatc gtcatggcgg ctatacgccg accccggtga
      661 tctcccatgc gattcttact tataatcgcg gccgctcatc cgggcacagt gacggtatcg
      721 tgatcacgcc gtcccataat ccccctgaag acgggggcat caaatacaac cctccacaag
      781 gcggcccggc cgatacgaca gcgaccaaat ggatcgaaga gcgggccaat agcttgctgg
      841 cggccaagct gcagggtgtg atccggcgag ccggatcatc gagcaagcgc aatccattgg
      901 tacatgagca tgactatatt ggggcctacg tcgcagatct ggagcacgtg atcgatttcg
      961 agccgatccg agccagcaag ctcaagttgg gcgtcgaccc gctcggcggc tcgggagtcg
     1021 cctattggga accccttgca gaacgctatc gcttgaatat cgaaatcgtc aatgcgagcg
     1081 tcgatccgac ctttcggttc atgccgctgg attgggacgg cagaatccgg atggattgct
     1141 cctctccata cgccatggcg aatctgatcg cactcaaaga tcgatttgat gtggcgttcg
     1201 gcaatgatgc ggacaacgac cgtcatggta tcgtcacggg ctcggccggt ctcatgaatc
     1261 ccaatcacta tctggctgcg gccattgcct atctcttctc acatcggccg cactggcgcg
     1321 ccggggctcg cgtgggcaag acactggtca gtagcagctt gatcgatcgt gtcgcacaga
     1381 aactgcgacg catgctggtg gaagtgccgg tcggctttaa atggtttgtc gatggcctct
     1441 tggatggttc gctcggcttt ggaggggaag aaagtgcggg agcgtccttc ttgcggcgcg
     1501 acggtctcgt ctggaccacc gacaaggatg gcatcatcat ggatctgctg gcagctgaaa
     1561 tgatggcgac gactgggcgg gatccgtccc acctgtatcg cacgctcacg gttgaattgg
     1621 gtgagccggt gtatgcgcgt atcgacgcac cagccacccc tcagcagaaa gcggcgctct
     1681 cgaaactttc ctccgaccaa gtcccggccc agacgctcgc cggcgatccg atcctggcca
     1741 ggctcaccac cgcgcccggc aacggagccg cgatcggcgg actcaaggtg gtcaccgcgc
     1801 agggctggtt tgccgcccgt ccttcaggca cagaagacgt ctacaaactg tacgccgaaa
     1861 gcttcaaagg accggaccac ctctcgctga tccaggcgga agcgcaggcg ctgatcgcga
     1921 aagtgctgac gccggcttag cgggagacgt gctgatccgc atggtaggag ctgcggacta
     1981 agggaccgga ttcgacgtgg acaaagccca tggccagcgc ctcggtcttg aatgcggtga
     2041 aggaggccgg ctcgtaaaaa tgggcgaccg gaagatgctg ggtcgtcggc tgcaggtatt
     2101 ggccgatcgt gacgatgtcg cacccggctc gacgcaaagc gtgcaggaca agccggattt
     2161 cgtccggcgt ctcccccatc ccgatgatta gtcctgattt ggtggtcagt ctggcgcgct
     2221 tggcacgcgc caagacctcc agcgatcgct gatatttccc ttgcggacgc aggatcggaa
     2281 acagccgtgg aacggtctcg atgttgtggt tcaagacatc cggctgggct tcgaagatca
     2341 cgtcgagtgc ggcggcgttg ccttccagat caggaatcag cacttcaatc gtgcaatccg
     2401 gattgacccg cctcgtgtgg cgaatggttt cagcaaagat cgccgcgccg ccgtccgcta
     2461 attcgtctcg attcacggag gtgataacgg cgtgtcggag acgcaatgcc ttgacggctt
     2521 cggcaatccg acgcggttca tcctgatcga cggcgccggg gcgaccggtc gagaccgaac
     2581 aatagtgaca ccgcctcgta cagacgtcgc ccaagatcaa aaacgtggcg gtgcgggcat
     2641 tccaacactc ccaccgattg ggacagcggc cctcctcaca gatcgtgtga agcttgaggc
     2701 gatcgacggt ctgcttgatg tccaagtagt ccgaaccggt cctggcttcg acgcggaacc
     2761 acgccggcag gcgtggcctc gagtgcgagc gcagggtctc gatcgaaatc aaactcttgg
     2821 gtgagtcgct agtcacttcc gtgaaaccag ttacggattg tcgacagcag ccccgagcct
     2881 cgatccggcg ccggatcggc atactcgatc cagggttcct gtgagcccag gtacgttccc
     2941 ttccgaaaac tcaattgggt tttgagttga cgatacccct gcgcatgcag gtcgtggatc
     3001 aacaccagca aggcggccgc ttcggtcgca gcgggcccgg tgacgatctg cgtagactcg
     3061 tatcgcagtc gagcacggta ctgcccctcc acgggatcaa tatcgactgg ccggctgaag
     3121 cccctctgac ggtcgtcaag cccggcgagc tggaagcggt caaccaaact gttgatggcg
     3181 tccttcttct catccattcc aggttccggc gaatgtagac accctccccg ccccccacgg
     3241 ggtttgccag atatttgccg ggacgtgccc cgctccacac agcgcgtgcg tgagagggag
     3301 aggctgcgcc tctgcgtttt ttcttgccgc cctgcagcgt ttctgcacag tgtcctgcgc
     3361 atgcaattcc ggctagtcca gcgcccggaa cacggcaacc agatcgctga gcgcgagcgt
     3421 tttctgcgtc aagaccgtgc gctcggcggc cagtctctcg tgcagttggg cgttgccagg
     3481 gccggctttc aggcaggagg cgcccagcgc aaggattcga tcgcattctt cggttaattt
     3541 cttctttacg actggattga ctgagaggat gctcaataat ctgcggcgag tctcggtcaa
     3601 gtgagcctca agctcggagt ccggatagct ggctcgggct acgtcgtcta cacagcgatc
     3661 gaggtattgc gagagaaaga catagagacg gacgagcgcg gcggcgattt cgatctggtc
     3721 ttttgcggtg gcaggcatcg cggtctggtg ttttcacggg gactgctaca gctggacttt
     3781 cacatcagag ccttcgactt tgaccggata cgtctggact cgggcggccg ggttgttcac
     3841 gcaggcaccc gtggtcacat cgaactgcca tccatgccat gggcaggtca cgacagtcgc
     3901 ttccagctcc ccttcaccga ggggacctcc acggtggacg catgtattgt cgatgacatg
     3961 aatctgtccg tcaacattga aaacggccag cgctttcccg ttaacctccg ctaccaggcc
     4021 ctgtccaggc tttacctcgt cgactgcagc aacgcgaacg aactctccca tcggccccgc
     4081 ctccctcctt gtgaccagtc gtcactgatt ggtaaatggt tgcttgatct ttttgagcag
     4141 gctgtcgatc gaagcgccgc cttttgccgc tcccgccaat gactccatga gttttttggc
     4201 gatctcccga aaggcggccg cctggggcga gccgggatca gcgacgacga ttgggtgccc
     4261 cgtgtccccg ccttcccgaa tgaccgggtc aatcgggatg ctccccagaa agggaattcc
     4321 cagtttggat gcggcgcgct ctcctcctcc gtgagaaaaa atgtccgtcc gctcgccgca
     4381 gtggccgcac acaaaaaagc tcatgttctc gataatgcct aacagcggca cattgacctt
     4441 ttggaacatg gccatgccct tgcggacatc gtagagagcg acttcttgcg gcgtggtgac
     4501 tgtaatcgcc ccggccaacg gtaccatctg ggataaggac agctggacat cgccggttcc
     4561 cggcggtaga tcgatcagaa gatagtcgag gtctccccag agcacgtcgc ggaagaacgc
     4621 ctggaggtat tgatgcacca tcggcccacg ccacacaagc ggcgcttctt cggggacgag
     4681 atacgccatt gagatgagtt tcacgcgatg gttctcgacc ggaacaatct tcccgtctcg
     4741 ttgctcaggc ccggccgtac tgcccatcat catgggaatg ttcggcccat agagatcggc
     4801 atccatcagg ccgaccttgg cgcccgtcag ggccagcgcg caggcgaggt tggcggcaac
     4861 agtggatttg ccgacgccgc ccttcccact actgatcgcg atgacatgtt tgacgcccgg
     4921 gatcagattt tctttgggtt gggccgcttg ctcttcgtca gccatacacg actccttatc
     4981 gcgcgaaatc acacgctatg ctgcacgcgt gtggctgtgc tcctgtcgtc atgggtcgga
     5041 caggatctga atagggtcag tatcggttcg accactccct actacttcat cataagggat
     5101 acagaagccg aaagaaaatg ggcccattgg ccctggcagg accgctcgag agtctattcg
     5161 gacaagacag tcgaatcgag gcggcgaaga ttggtcgcca atccgagcat ggagaggcag
     5221 taaattgtcc acttggacgg atcaaagttg taccagcgag ggccgttgcg gtaatcgcgc
     5281 gcgtaggtgt ggtggtaatt gtggtacccc tcaccgaagc tgaggaaaga gaccagccag
     5341 ttgtcgcggc tgctgtcccc cgttccgtgt ggttgcgttc cgaacatatg acacagggag
     5401 ttgatggcaa aggtcgaatt gagcaccatg aacatccgga acattccggc gaggaaaaaa
     5461 cacccgatgc cgccgatcag tccgccgtgg aggtagccca gcagcaaggg aaacgctaac
     5521 ccggagatca cgaccgcatg atagtgccgg tgctgccaca taacgatggg atccttgcgc
     5581 aattgccgct cgtagttttc cgtccggtgc gaggtgttga tgaagagcca aatgcagtgg
     5641 ctatgccaaa agcctttctg ggcattgtag gggtcttctt cctggtccgt cctcgcgtga
     5701 tggcgaagat ggtcggcgct ccagcgaagg gcggaatttt gcagcgccca tcctccggcg
     5761 attaacagca gggctttgac ccagggatgg cactcgaagc tgcgatgtga aatcaaccga
     5821 tgatagccga ccgtgatgcc caggcccgta cacacgtaga ggatggcgaa gagggcccag
     5881 tcaaacagcg tatagccata gagatagccg aagagcggtg ttccgatcag cgtgacggca
     5941 acgacgagac aaaagagcgt gaacgtctta taggcaaacg gggtggttcc aacagcgtcc
     6001 ggcattctca ctccttctga acattcaaca gtaggttatg tcgttgtcgc tggctcgggc
     6061 acgttgttta gcagcacgca gagcttctca gcccactcaa agagctatat gcactccggc
     6121 gtgttaggga attcacgacc gctctggccg ggactgaagt ctcgctgagt aagagtacac
     6181 aagtagcaga ctttaatcaa gaaattcttt gcctgccgcc ggggcgagca ccagacggag
     6241 gtgtatactg aacggtgtga gaacgtgatc cgtaagccgg taggtccgcc gtggctgcca
     6301 ttgatcctct ggaatcttct gtccaacaca ttggaggaga actcgcccga ctctctgcag
     6361 ggcaggctcc tgctcttttt gaacgtcaat ggtggtcgca agcggccatc aatctggcga
     6421 tgcatgacct cgacttcaag acacagctgt tccgtttcat tgacgtcttg ccgtcggtcg
     6481 ccgacgatca acgggtcgtg accctggcac aggaatactt cggggcgatg gccgatcaag
     6541 cttttggtct tcgctggggg ttgaaggccc tgtctgcaac gagcatcggc gcccgtctga
     6601 gcggacgggc cattcgtgcc caggtggagc agatggcgcg gacctttatc gccggctcgt
     6661 caatcgagga tgccgtgccg gtcctgggcg cattgtgggc gagtggcaag gcctggtccg
     6721 tcgatctgct cggcgaagcc acaatcagcc accaggaagc cgatcgctac cgggatcgat
     6781 gtgtggctgc cctggacagc ttcgctcagg ccgcgcgagt ctggccggcc attccgcttc
     6841 tggagaacga tcatctcggt gccgtgccgc gggtgcagct ttcactcaag atttctgctc
     6901 tgtctccctt tctcgatccg attgatcccg aggccagctt tgaatcggtc gcgtcacgcc
     6961 tgcggcccat cgtggatatg gccatgcgag cgccggcttc gctgatcttc gacatggaac
     7021 aagccgatac aaaatcgctg gtgatcgaga tcttcacacg gctgttcacg gaaacgccct
     7081 atcggcagtt tcctcatgcg ggattggccc ttcaggctta ccaccgagag accactcagg
     7141 atgtggcatc cctgcttgga tgggttcgca cgcggggcgt gccgattacg atcaggctgg
     7201 tgaaaggcgc ctactgggat tcggacacaa ttcgttaccg ccagcggggc tggccggtgc
     7261 cgctatttga agagaaggcg gagaccgact tcaactacga atcgttggtg cgcatcctgt
     7321 tggaggcatc gtccctcatc cggccggcgt tcggcacaca caacctgcgc acactcgcct
     7381 atgtcgaggc cgtggctgag cagctggggc ttcccccttc gacgtgggag taccagatga
     7441 tcttcggcat ggccgagccc ttccagcagg ccgtgttgcg gcgaggccgt cgtctgcggc
     7501 tctatactcc ggtcggcgat ctgcttcccg gcatggccta tctcgtgcga cggctcttgg
     7561 agaatacgtc gaatgaatcc ttcttgagaa aggaatatgt ggagtcgcag cc
//
LOCUS       DASSCQ010000229        11173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_170398, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000229 DASSCQ010000000
VERSION     DASSCQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170398"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFH05_14445"
     CDS             <1..1047
                     /locus_tag="VFH05_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HET6679471.1"
                     /translation="GPTVDDCTREAVAQAAGRRLARRKEAFEVMQARLAQWGRVPNRG
                     QLRQALIPSGASVVPNPVGSAPGFWLSWRGALLIALPGVPGEMAAMMRETVVPLLASH
                     VPQAPGRSIRRLIFHTWGIPEADVDAKLLGLIPHAIPVDLGLLASPMGVLVSLTMKPR
                     AAAGDDALVSVSDEVRARLQEWIFAEGQESMEEVVGRLLAKQHRTVALAESCTGGLIG
                     HRLTQVPGSSSYMDRGVICYSNRAKVELLGVRPELIEQQGAVSAEVAAAMAKGVRERS
                     GVSVGLSVTGIAGPGGGTAAKPVGLVYIGLNADDGTSLVKEHRFHGDRTVIKQRSSQA
                     ALDLLRRWLLAGHP"
     gene            1133..1735
                     /gene="thpR"
                     /locus_tag="VFH05_14450"
     CDS             1133..1735
                     /gene="thpR"
                     /locus_tag="VFH05_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET6679472.1"
                     /translation="MIRTFLAVELSELLRAHLATIQADLKQRMANALTRNVSISWVRP
                     ASLHVTVKFLGDIDEGLVLPLKEAIAELLSAYRPIAVPVTRLGVFPRPQQPRILWAGP
                     SEQWERGEDASRLAALHRAIDACCGSLNLAPDSRPLSAHLTLARVKAGERVVGQLLAQ
                     SGVMDRPFTCESLDINAIALMKSELKPSGSVYTRLWDVRL"
     gene            complement(1852..2937)
                     /locus_tag="VFH05_14455"
     CDS             complement(1852..2937)
                     /locus_tag="VFH05_14455"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6679473.1"
                     /translation="MSHASVQDTADRKPTVLIVDDEAAPRAALTQILKEDFNILTAKD
                     GRHALAVINDRGVDLITLDLKLPDTSGADILKEIKRTHREIEVIMVTAYGSLQSAMDC
                     VRHGAAGFLLKPFNAGELLSISLQTSQKKQRLDMLREAMATHEALWQPDPGCTEAWQA
                     LLARYARQVKSGLLEVSSREVTSPLIQLVSDLLEARDRHLVNHGNRVSFYATLVGNKL
                     QLPLPEQQYLSLGALAHDLDLICHDSSPVLHHDSTSTQHRPDLGARIGRAIGLPSEAV
                     QIIALHHERWDGTGYPFALKEERIPYLARLVSLAQTFDELTADRPGAVPVPFKEALEV
                     IEAQSGTAFDPSLVEVFCRTIREQPPK"
     gene            3056..3448
                     /locus_tag="VFH05_14460"
     CDS             3056..3448
                     /locus_tag="VFH05_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679474.1"
                     /translation="MKWLMVITVLVSGLCTTAWAGESGGFDPEQPFKDAFNQRSLQSF
                     LNQALEALEDHFEITGNLNSDSGEGDRTNKLQFKFYPEGKSKSNDHVTAEGWFGPSKD
                     SLQDEFHFRFAVPKSSSKPSAEPLTNVL"
     gene            3564..4646
                     /gene="recA"
                     /locus_tag="VFH05_14465"
     CDS             3564..4646
                     /gene="recA"
                     /locus_tag="VFH05_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET6679475.1"
                     /translation="MAEKDDKKRALDLALSQIEKQYGKGAIMKLGDAEAPADIPAISS
                     GSLGLDIALGVGGFPRGRVIEIFGPEASGKTTLTLHAIAEVQKTGGVAAFIDAEHALD
                     LTYARKLGVQADDLLVSQPDTGEQALEIAETLVRSGAIDVIVVDSVAALTPRAEIEGE
                     MGDAHMGLQARLMSQALRKLTGAISKSQTTVIFINQIRMKLGVMFGNPETTTGGNALK
                     FYSSVRLDIRRIESIKEGQDVVGSRVRVKVVKNKMAPPFKQAEFDILFSQGISKAGEL
                     VDLGVDKKVLEKSGAWYSYHGERLGQGRDAVRDFLLTNQPLAREIEGKLRDLAGVPGR
                     GVEKPALAPAKEDNKRDEKRPHKVGV"
     gene            4671..5153
                     /locus_tag="VFH05_14470"
     CDS             4671..5153
                     /locus_tag="VFH05_14470"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET6679476.1"
                     /translation="MKRRIPQSGDPWFQCAVRYLAQFDRTAAQVERFLTRKGASSAQA
                     KQILSRLSTLRYLDDRAYAARWIETTLARRPMAAERLKSELLARGLSEAVADEMINKG
                     MEAVDEEALARRVVLLQSRQGRRFLPQRVAGLLRRRGFAEDTIERIIRDCTQTEGSPP
                     "
     gene            5150..7783
                     /gene="alaS"
                     /locus_tag="VFH05_14475"
     CDS             5150..7783
                     /gene="alaS"
                     /locus_tag="VFH05_14475"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET6679477.1"
                     /translation="MTQRARELRQAFIRYFEEHGHQAVPSSSLIPQADPTLLFTNAGM
                     NQFKRVFLGQETRSYHRAVSSQKCLRAGGKHNDLENVGYTRRHHTFFEMLGNFSFGDY
                     FKEDAIRFGWEFLTKSVGLDKSRLWITVFREDDDAEELWKKVGIAPSRIIRCGEKDNF
                     WQMADTGPCGPCSEIHFDQGPAVPGDDRPNGEGDRVIEIWNLVFMQFNRDASGTLHPL
                     PKPSIDTGMGLERLAAVAQGVHSNYDSDLFLPLLSAIAARAGREYGKQEQTDRSMRVV
                     ADHLRAITFLMTDGILPSNEGRGYVLRRILRRAARHGRLLGIVEPFLHDLTGTVVQQM
                     GDAYPEVRNAAPTVAEATKGEEERFIATLDQGLPILSELIAKTRSAGGTILPGADVFK
                     LYDTYGFPMDLITEASREQGMTVDEAGFDAAIEEQRTRARKTSGFEQEIARPAVADLA
                     KRVGATTFVGYDQLESDSIVQAILKGDRLVKEAHEGDEVEVVLDVTPFYAEGGGQVGD
                     QGVLVGAEGRLDIKETTRPVATVILHKGTVTTGRIREGEQLHLSVNRTTRQDAARNHT
                     ATHLVHAALRDLLGPHVKQFGSLVAPNRLRFDFAHFRPLSSRDIDEIETVVNGEIRRN
                     ETVRSEVMSIQDAVAKGALAFFGDKYGDEVRVVTVESFSKELCGGTHCRHTGEIGLFR
                     IVSETGVAAGVRRIEAQTGSGAMAMMKKLETEVRELSELLKVGPSELVAKTRKVMAQL
                     KDKERELEELKLKMASGSGVSATARTIAGVPVHVQRTDGLDMNGMRALADQLRDKLKT
                     GVVALGAAMEDGKVSLLVVVTKDLTGKIKAGDLIKVMAAEVGGTGGGRPEMAQAGGKD
                     SSKLDASLEKVFGLVEHALQR"
     gene            7788..8333
                     /gene="ruvX"
                     /locus_tag="VFH05_14480"
     CDS             7788..8333
                     /gene="ruvX"
                     /locus_tag="VFH05_14480"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET6679478.1"
                     /translation="MAGPRILALDYGTKRIGVALSDELGWTAQPLETFERRTLDRDVA
                     HIVSLVNTHEVGEVVLGFPLQLDGREGPAILAMRDFASRLEQGLPVPLVLWDERLTTK
                     AAEDLLIAADVSRKKRKGTVDRVAAAILLQSYLASRESAEHRVVESDAQTIDKTDEAS
                     EHFSHSAGRRRRSRHGRVSDH"
     gene            8257..9291
                     /gene="mltG"
                     /locus_tag="VFH05_14485"
     CDS             8257..9291
                     /gene="mltG"
                     /locus_tag="VFH05_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET6679479.1"
                     /translation="MKLRSIFLILLVGGAVLGMAGYQIIKWAQGPAVSARLNPPNQLI
                     RIPDRSSFQQVAALLERERLIRSRAAFVWMGRLQSADRKIQAGEYELNPSMLPAEILA
                     RLLNGQVVLHPVLIPEGLTMVQIADIFGQQGLVDREELLRLMKDPAFIASLGIRADTL
                     EGYLYPDTYKFPKSVKPKDVLVTMVEHLRQVYTADFHARAQELKMSQHEVLTLASVIE
                     KESASNGEREEIAAVFHNRLKKHIPLQSDPTVIYGLPNYDGNIHKKDLLSPSPYNTYR
                     ISGLPPGPIANPGIQSIRATLYPSDSRALFFVSKNDGTHQFSTTLAEHNQAVEKYQKR
                     PFRRAGHPRT"
     gene            9372..10052
                     /gene="deoC"
                     /locus_tag="VFH05_14490"
     CDS             9372..10052
                     /gene="deoC"
                     /locus_tag="VFH05_14490"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET6679480.1"
                     /translation="MKQTEWDLPALIDHTILRPEATRADVLRLCREAKEQGFRVIFIP
                     PCYAAEAVVAVSGSPVRVGIPIGFPLGGHTTGTKVAEALEGVRQGASVLDMVINVSRL
                     KSGDHDAVRQDIAEVVLATPQAEHKVILETCYLSDQEKMTACRLAVEAGADYVKTSTG
                     FGASGATVSDVKLMVAAVQGKAKVKASGGIRDWNMTRQLLEAGADRIGTSASLAILDE
                     WSAARATR"
     gene            10059..10382
                     /locus_tag="VFH05_14495"
     CDS             10059..10382
                     /locus_tag="VFH05_14495"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6679481.1"
                     /translation="MALRRDVRYQVQCPVAFVVDGKPGQGTTFNLSRGGCAIDSDLFA
                     AIDDPISLQITVSSQPTPIAIELGKVRWATRRELGVEFMVVERASKQRLDDFLLAVAK
                     EKLTS"
     gene            10482..>11173
                     /locus_tag="VFH05_14500"
     CDS             10482..>11173
                     /locus_tag="VFH05_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679482.1"
                     /translation="MIKRVILLVVEGLGVGATDDASTYGDTSCHTLAHLAESAGGLSL
                     PTLESLGLGHVTTIQGVRSMAQPVGCFGRLGFKSKGVDSLVGYWEIAGCVSEEEGRTF
                     PNGYSTAAIAALEAALGHKVVGGRVASGEEALKEFGARHLSDGAPIVWTDGRQTCHVA
                     AHDSVWSGEVLYQRVRESRKALRDAWGMRRLVAHPLIGAVGAVRFGPGRKDYAGEPPG
                     QTMLDLLNRASQ"
BASE COUNT         2310 a         3105 c         3579 g         2179 t
ORIGIN      
        1 gggccgacag tggacgactg cacgagagag gcagtcgcac aagcggccgg gcggcggctg
       61 gcgaggcgga aagaagcgtt cgaagtgatg caggcccgct tggctcagtg gggacgagtc
      121 cccaacagag gacagttgcg acaggccctg attccgtccg gggcgtcggt ggtgccgaat
      181 cccgtcggat ctgcgccggg attctggctg tcctggcgcg gggcgctgct gattgcgctg
      241 ccgggcgtgc cgggcgaaat ggccgccatg atgcgggaga cggttgtgcc cttgttggca
      301 tctcacgtgc ctcaagcccc cggccggtcg attcggcgtc tgatctttca cacatgggga
      361 atccccgaag ccgatgtcga tgcgaagttg ctcggactca ttcctcacgc catccccgtt
      421 gatctcgggc tgttggcttc gccgatggga gtgctggtct ctctgaccat gaagccgcgg
      481 gccgccgccg gggacgatgc cctcgtctcg gtatccgacg aggtgcgcgc ccgtctgcag
      541 gaatggattt ttgccgaagg ccaggagtcg atggaggaag tggtcgggcg gttgctcgcg
      601 aagcagcacc ggaccgtggc tctggccgaa tcctgcaccg gcgggttgat tggtcatcgc
      661 ctgacacaag ttcccggatc ctcctcttat atggaccgtg gcgtgatctg ttacagcaat
      721 cgcgcgaaag tcgaactgct cggtgtgcgg ccggaactga tcgagcaaca gggcgcggtg
      781 agcgccgagg tcgcggcggc gatggcgaag ggcgtgcgag agcgcagcgg cgtgtcggtc
      841 gggttgagcg tcacgggcat tgccggcccg ggcggtggaa cagcggcgaa gcccgtcggg
      901 ctggtctaca tcgggctgaa tgcagatgac ggcacgtcgc tggtgaagga acatcgattt
      961 catggcgaca ggaccgtgat caaacaacgc tcttcccagg cggcgctgga tctcctgcgg
     1021 cggtggttgc tggcagggca tccctaaacc ccgccagccg ttctccgtcg ctgagctcaa
     1081 catgcgggag aacccgccat tctagacaac ctgcaggagc ggatgagaga gcatgatacg
     1141 cacgttcctg gccgtcgaac tttcggaact gctaagagcg catctcgcca ccatacaagc
     1201 agacttgaag cagcgcatgg ccaacgcact gactcgaaat gtaagcatct cctgggttcg
     1261 gccggcctcc ctgcatgtga cggtgaaatt tctcggcgat atcgatgaag ggttggtcct
     1321 tccgctgaag gaagccatcg cagaactgct gagcgcgtac cgcccgattg cggttcccgt
     1381 gacccgtctc ggcgtgtttc cgcgccccca acagccgaga atcttgtggg cagggccttc
     1441 ggagcaatgg gagagaggag aggacgcgtc gcgattggcc gcgctgcacc gtgctattga
     1501 tgcctgctgc ggatcgctga atctggcccc ggacagccgg ccattgagcg cgcacctgac
     1561 cttggcccgc gtgaaagccg gtgagcgggt cgtcggacaa ctcctcgctc agagcggcgt
     1621 gatggatcga ccgttcacct gtgagtcgct ggacatcaac gcgattgcct tgatgaaaag
     1681 cgaactgaag ccgagcgggt ccgtctatac acgattatgg gacgtgaggc tgtgaggatg
     1741 gtaagacgct tcggcgaacg cagtgacagc ccatgcgacc catcgcaagt cgcagtccag
     1801 cttcaccatc ctcttcagtg aaaagaaaaa tagggcggaa agacggcaat cttatttggg
     1861 tggctgttcg cgaatcgtac ggcagaacac ttcgacgagg gacggatcga atgcggtgcc
     1921 ggattgcgct tcgatcacct cgagcgcctc tttaaaaggt acgggcacgg cgccgggtcg
     1981 atcggcagtg agttcgtcga acgtttgggc aagcgagacg aggcgagcca agtagggtat
     2041 gcgttcttct ttaagggcga acggatagcc agtgccgtcc cagcgttcat gatggagggc
     2101 gataatctgc acggcttcag acgggaggcc gatggcccgc ccaatgcgcg caccgagatc
     2161 cggacgatgc tgcgtcgagg tgctgtcatg gtgcaacacg ggactgctgt cgtggcagat
     2221 caggtccaaa tcatgagcca gagccccaag cgaaaggtat tgctgttcgg gcaagggcag
     2281 ctgcagctta ttgcccacga gagttgcgta gaagctcaca cgattgccgt ggttgaccag
     2341 gtgtcgatcc ctcgcctcca gtaagtccga gaccagttgg atcaggggcg acgtgacttc
     2401 ccggctcgac acttccagca agccgctctt gacctgtctg gcgtatcggg ccaggagcgc
     2461 ctgccaggcc tccgtacagc ccggatccgg ttgccacagc gcctcatgcg tcgccattgc
     2521 ctcgcgaagc atatcgagcc gctgcttctt ctgcgaggtc tgcaggctga tggacagcag
     2581 ctcgccggca ttgaagggtt tgaggaggaa gccggcggcg ccgtgacgca cacaatccat
     2641 tgcggattgg agcgagccgt acgccgtgac cataatgact tcgatctccc gatgtgttcg
     2701 cttgatttcc tttaagatgt cggctcccga ggtgtcggga agtttcaggt ccagtgtgat
     2761 caggtcgacc ccgcggtcgt tgatcaccgc cagcgcatga cgtccgtctt tggccgtcag
     2821 gatattgaag tcctctttca aaatctgtgt cagagccgcc cgaggcgccg cttcgtcatc
     2881 gacgatcaag accgttggtt tacggtcggc tgtgtcttgg acggacgcgt gtgacatacc
     2941 ttcagctgta tgatggcaag ccggtttcgt caaggaaatc acgattgggc gttgtaggag
     3001 aaagcctgag ccagatgcac ttgactgacc acacttcaca ttatagagtg tcaccatgaa
     3061 atggctgatg gtcatcacgg tactggtctc cggcctctgc acaacggcct gggcagggga
     3121 aagcggcggg ttcgatccgg agcagccgtt caaagacgcg ttcaatcagc gatcgctgca
     3181 atccttcttg aaccaggcgt tggaggcgct ggaggatcat ttcgagatca ccggcaatct
     3241 gaattcggat tctggtgaag gcgaccggac gaacaagttg caattcaagt tctatccgga
     3301 aggaaagtcg aagtcgaatg accatgtgac cgctgaaggg tggtttggtc cctcaaagga
     3361 ctcactgcag gacgagttcc acttccgctt tgccgtgccc aagtcatcca gcaagccctc
     3421 cgctgaaccg ttgaccaacg tgttgtaggc ctcttcgtct gtattgcggc cattttcttc
     3481 ctcccatcac attctctgct aggtttctct ccgccaactt gggtataatc cgccacgctt
     3541 caccctgatg aggaggattt tccatggcgg aaaaagacga caagaaacgc gcgttggatc
     3601 tcgctctatc ccaaatcgag aagcagtatg ggaaaggggc gatcatgaag ctcggcgatg
     3661 ccgaggcgcc ggcggacatc ccggcgattt cttcgggatc gctcggcctg gacatcgcgc
     3721 ttggcgtcgg cggatttccg cgtggccgcg tgattgaaat tttcggacct gaagcctcag
     3781 ggaagacgac gctgaccctg catgcgattg ccgaagtgca aaagaccggc ggagtcgccg
     3841 cgttcatcga tgcggagcat gcgctcgatt tgacgtacgc aagaaagctg ggcgtgcaag
     3901 ccgacgatct gttggtctca cagcccgaca cgggcgagca ggcgttggaa attgctgaga
     3961 cgctggtccg tagcggtgcc atcgatgtga tcgtcgtgga ttcggtggct gccttgaccc
     4021 cccgtgcaga aatagagggt gaaatggggg atgcgcacat gggcttgcag gcgcgcctga
     4081 tgtctcaagc ccttcgaaaa ctgaccggtg ccatttcaaa atcacaaacc acggtgatct
     4141 tcatcaatca gattcggatg aaactcgggg tgatgttcgg caatcctgaa acgacgacgg
     4201 gcggcaatgc gctcaagttc tactcgtccg tacgactgga cattcgccgg atcgaatcga
     4261 tcaaggaagg gcaggacgtg gtcggcagca gagtccgcgt gaaggtcgtg aaaaacaaga
     4321 tggctcctcc gttcaagcag gcggagttcg acatcttatt ctcgcagggc atttcaaaag
     4381 ccggcgaact ggtggatctc ggcgtggata agaaagtgtt ggaaaagtcc ggcgcctggt
     4441 attcctacca cggcgaacgt ctcgggcagg gccgtgatgc ggtccgcgac tttttgctga
     4501 ccaatcaacc cttggctcgc gagatcgaag gcaagcttcg ggacctggcg ggcgtgcctg
     4561 gacgcggcgt cgaaaaaccg gcactcgcac cggccaaaga agacaacaag cgggacgaga
     4621 agcgccccca caaagtcggc gtctaacgga ctccgtcaat aggcgggaga gtgaaacggc
     4681 gcattccgca gtccggggac ccatggttcc aatgtgccgt gcgctatctt gcacagttcg
     4741 acagaacggc ggcacaggtc gaacgttttc tcacgcgcaa gggcgcgtca tccgcacaag
     4801 ccaagcagat cctcagccgt ctctccacgc ttcgctatct tgacgatcgg gcttatgccg
     4861 cacgctggat cgaaaccacg ctggcacgac ggccgatggc tgccgagcgc ctcaagagcg
     4921 agttgctggc cagaggcctg tctgaagcgg tggccgatga aatgatcaac aaaggcatgg
     4981 aagcagtcga tgaagaggca ttggcacggc gggtagtgtt gcttcagagt cgtcagggtc
     5041 gccggtttct tcctcagcga gtggcggggc tcctacgtcg gcggggtttc gcagaagaca
     5101 cgattgagcg tatcataagg gattgtaccc agaccgaggg atcaccgcca tgacgcagcg
     5161 tgctcgagag ctcaggcaag cgttcatccg ctacttcgag gagcatggcc accaggccgt
     5221 gcccagttcc tctctcatcc cgcaggccga tccgaccctg ctgttcacga atgcgggcat
     5281 gaatcaattc aaacgcgtct tcctggggca ggaaacgcgg agctatcacc gggccgtgtc
     5341 gtcgcaaaag tgcctgcgcg ccggcggcaa gcacaacgat ctggagaacg ttgggtacac
     5401 gagacggcac catacgttct tcgagatgct cggcaatttc tctttcgggg attatttcaa
     5461 ggaagacgcg atcaggttcg gctgggaatt cctgacgaag tcggtggggc tcgacaagtc
     5521 ccgcctctgg atcaccgtgt ttcgtgaaga cgacgatgcc gaagagttat ggaagaaggt
     5581 cggcatcgct ccttcgcgca tcatccgttg cggagagaaa gacaacttct ggcaaatggc
     5641 ggataccggc ccgtgcgggc cttgctctga gattcatttc gatcaagggc cggctgtgcc
     5701 cggcgacgac cggccgaacg gcgaagggga ccgggtgatc gagatttgga atctcgtctt
     5761 catgcagttc aatcgtgatg catcgggaac gctccatcct ctgcccaagc ccagcattga
     5821 cacgggaatg ggattagaac ggctcgctgc ggtggctcag ggtgtgcaca gcaactatga
     5881 cagcgatctt tttttgcctt tgctgtctgc catcgccgct cgagccgggc gggaatacgg
     5941 caaacaggag cagaccgacc gttccatgcg cgtcgtggcg gaccatctgc gtgccatcac
     6001 cttcttgatg accgacggca ttcttccatc caatgagggc cgcggctacg tgctgcggcg
     6061 tattctgcgc cgcgccgccc gccatggcag actcctcggc atcgttgaac cctttctcca
     6121 cgacctgacc gggacggtcg tccagcagat gggggacgcc tacccggaag tgcgcaatgc
     6181 cgctccgacc gttgccgaag cgacgaaggg cgaagaagaa cgcttcatcg ccacactcga
     6241 tcaaggcttg ccgattttga gcgagctcat tgcgaagacc cgctcggccg ggggtaccat
     6301 cctgcccggg gcggatgtgt ttaaactcta tgatacgtac ggcttcccga tggatctcat
     6361 taccgaggcg tcccgggagc aaggcatgac ggtggatgag gcgggatttg atgccgcgat
     6421 tgaggaacag cggacgcgcg cgcggaaaac gagcggattc gagcaggaaa tcgccaggcc
     6481 ggccgttgcc gatttagcca agcgcgtcgg tgccacgacg ttcgtgggtt acgaccagtt
     6541 ggagtccgac agcatcgtgc aagcgattct caaaggcgat cgcctcgtca aggaagcgca
     6601 cgaaggggat gaggtcgaag tggtgttgga cgtgaccccc ttttatgcgg aaggcggcgg
     6661 gcaagtgggg gatcagggcg tgcttgtagg ggcggaaggg cgtctggata tcaaggaaac
     6721 gacccgtcct gttgcgacgg tcatcctgca caagggaact gtcaccaccg ggcgcattcg
     6781 agaaggggaa caactgcacc tctcagtgaa caggaccacc cggcaggatg cggcgagaaa
     6841 ccacaccgca acccatttgg tccatgccgc cttgcgggat ttgcttggtc cacacgtcaa
     6901 acagttcgga tcgctggtgg ctcccaaccg gcttcggttc gacttcgctc atttccgtcc
     6961 gctgtcttcg cgtgatattg acgagattga aacggtcgtc aacggcgaga tccgcaggaa
     7021 tgaaacggtg cggtccgagg tcatgagcat tcaagatgcg gtggcgaagg gggcattggc
     7081 gttcttcggc gacaaatacg gcgacgaggt gcgcgtcgtc acggttgaat cgttcagtaa
     7141 ggaattgtgc ggcggtacgc actgccggca cacaggtgaa atcggactgt tcaggatcgt
     7201 ctcagagacc ggcgtggccg ccggcgtccg gcggatcgaa gcgcagaccg gcagcggcgc
     7261 catggccatg atgaaaaagc tcgaaaccga agtcagggaa ctgtcggaac tcttgaaggt
     7321 cgggccttcc gagttggtgg ccaagacacg caaggtcatg gcgcaactta aggacaaaga
     7381 gcgggaattg gaagagttga aactcaaaat ggccagcggc tccggcgtgt ccgccaccgc
     7441 aagaaccatt gccggagtgc ctgtgcacgt gcaacgaacc gatggccttg acatgaacgg
     7501 catgcgtgcg ctggccgacc aactgcggga caagctcaaa accggcgtcg tcgcgttggg
     7561 agcggcaatg gaggacggca aggtttcctt gctcgtcgtg gtgacgaaag acctgaccgg
     7621 aaaaatcaaa gccggtgacc tcatcaaggt gatggcggca gaggtcggcg ggacgggagg
     7681 gggacgtccg gaaatggccc aagccggcgg gaaagactct tcaaagctcg atgcctcgtt
     7741 ggaaaaggtc tttggcttgg tcgaacacgc gctccagcgg taatattatg gctggaccac
     7801 ggatactggc gttggattat gggaccaagc gaatcggcgt tgcgctgagc gatgaactgg
     7861 gctggacggc acagccgctg gaaaccttcg agcgacgcac gctcgatcgt gacgtggcgc
     7921 acattgtctc cctcgtcaac actcatgagg tcggggaggt cgttctcgga tttccgctgc
     7981 aattggacgg tcgggaaggc ccggccatcc tggccatgcg ggattttgcc tccaggctgg
     8041 agcagggatt gcccgtgcct cttgtcctgt gggatgagcg gttgacgacc aaagcggcag
     8101 aagatctgct catcgccgct gacgtgagcc gcaagaaacg aaagggaacc gtcgatcgcg
     8161 tagccgccgc cattctgctt caaagttacc tggcgagccg cgaatcggct gagcatcggg
     8221 tcgttgagtc tgacgcccag acgatagaca agactgatga agcttcggag catttttctc
     8281 attctgctgg tcggcggcgc cgttctcggc atggccgggt atcagatcat taagtgggcc
     8341 caaggtcccg ctgtttctgc ccgactcaat cctcccaacc aactcatccg gattccagac
     8401 cgatcgagct ttcagcaggt cgccgcgctg ctcgaacgtg aacggctgat cagaagccgg
     8461 gccgcgttcg tgtggatggg gcgcctccag tcggcagatc gaaaaatcca agccggggaa
     8521 tatgagttga accctagcat gctgccggcg gagattctgg cgaggctgct caacgggcaa
     8581 gtcgtgcttc atccggtcct catccccgaa ggcttgacaa tggtgcagat tgccgacatc
     8641 ttcggacagc agggacttgt cgatcgtgaa gaactgttgc ggctcatgaa agatccggcg
     8701 tttatcgcgt cgctcggaat ccgggcggac acgttggaag ggtacctgta tccggacacc
     8761 tataaattcc ccaaaagcgt caaaccgaaa gacgtgctcg tcacgatggt cgagcatctg
     8821 cggcaggtct acacggcgga ttttcacgcc cgggcgcagg agttgaaaat gagccagcat
     8881 gaggtgctga cgctggcatc cgtgattgaa aaagaatccg cgtctaatgg cgagcgagaa
     8941 gagattgcgg cagtgttcca taaccgattg aagaagcaca ttccgttgca gagcgatccg
     9001 accgtcatct acggcttgcc gaactatgac gggaacattc acaaaaaaga tttgttgagc
     9061 cccagtccct acaacaccta ccggatctcg gggctgcctc cgggcccgat cgcgaacccc
     9121 ggcattcaat cgatccgtgc cacgctgtat ccgtcggatt cccgggcgct cttttttgtg
     9181 tccaagaatg atgggacaca tcaattttcg accacactcg ccgagcacaa tcaggcggtc
     9241 gaaaaatatc agaagcgacc gtttaggcgg gcagggcatc cgcgcacctg agagggtgat
     9301 gctcaagtcc agcagtgggc tgggcgatga gatcgttaag ggttcatcat gacgtggagc
     9361 cagatcgcag catgaaacag acggaatggg atcttcctgc gttgatcgat cacacgatct
     9421 tgcggccgga agcgacgaga gccgatgtcc ttcgtctctg ccgggaagca aaggagcagg
     9481 gctttcgagt gatttttatt cccccctgct atgcggccga agcggtggtg gccgtttcgg
     9541 gctcaccggt ccgtgtgggc attcccattg gatttccgct gggcggacac accaccggta
     9601 cgaaggtcgc cgaagccctg gaaggagtgc ggcagggcgc atccgtgttg gacatggtca
     9661 tcaatgtcag tcgattgaaa tccggcgatc atgatgccgt ccggcaagac attgccgagg
     9721 tcgtgctggc gactccccag gctgagcaca aggtcattct tgaaacgtgc tatctcagcg
     9781 atcaagagaa aatgacggca tgccgactgg cggtggaagc cggcgccgac tatgtgaaga
     9841 cctcgacggg atttggcgcg agcggggcga ccgtgtccga cgtcaaactg atggttgcgg
     9901 ccgtgcaggg caaggccaaa gtcaaggcat ccggtggcat tcgcgattgg aacatgaccc
     9961 ggcagctcct ggaagccgga gccgaccgca tcggcacgag cgcgagcctg gccattctcg
    10021 atgaatggtc ggcagcacgc gcaaccaggt gacgagtcat ggccttgcgt agggatgtcc
    10081 gctatcaggt ccaatgtccc gtggccttcg tggtggacgg gaaaccgggg cagggcacga
    10141 cgttcaacct ttcgcgcggc ggctgtgcca ttgattcaga cctgtttgcg gccatcgacg
    10201 atccgatcag cctgcagatc acggtttcat cccagccgac gccgatcgcc atcgagcttg
    10261 gcaaggtccg gtgggccacg cggcgggagt tgggcgtgga gttcatggtc gtcgaacgag
    10321 cgtcaaagca gcggttggac gattttctct tggccgtcgc gaaagaaaaa ttaacctcct
    10381 gatgcgatca ttcccgccgc gctagccctg gtcacctcgc ctgtgatggc ttcactcatg
    10441 agacgatttg cggctcgacc cccatctgtt atagtgcgcc catgatcaaa cgcgtgattc
    10501 tgcttgtggt cgagggcctg ggcgtcggag ccacggacga tgcgtccacc tatggcgata
    10561 cctcctgcca tacgctggca cacctggccg agagcgcggg cggactcagt ctgccgacgc
    10621 tcgaatcgct cgggcttggc cacgtgacca ccattcaagg cgtccgatcg atggcgcaac
    10681 cggtcgggtg tttcggccgt ctgggattca aaagcaaggg agtggactca ctggtcggct
    10741 attgggagat tgccggatgt gtgtccgagg aagagggtcg aacgtttcca aacgggtatt
    10801 cgacagcggc catcgcagcc ctggaagcgg cactgggaca caaggtcgtg ggtggacgtg
    10861 tggcgtccgg tgaggaggct ctgaaagagt tcggcgcacg ccatctctcg gacggagcac
    10921 cgattgtctg gacggacggc cggcaaacgt gtcacgtggc cgcgcatgac agcgtgtgga
    10981 gcggtgaggt gttgtatcaa cgggttcgag agtccagaaa agcgctgaga gacgcctggg
    11041 gcatgcggcg gctcgtcgca cacccgctca tcggagctgt cggggcggtg cgattcggac
    11101 cgggccggaa ggattacgcc ggtgagccgc cgggccagac gatgctggat ctcttgaatc
    11161 gggcgagtca gat
//
LOCUS       DASSCQ010000230         4266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1623004, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000230 DASSCQ010000000
VERSION     DASSCQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623004"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFH05_14505"
     CDS             <1..500
                     /locus_tag="VFH05_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET6679483.1"
                     /translation="PVSKKEAEHIQAVHAKAFAEQASSLRILPGAQELLDTLAAEGVA
                     HAIATSGRLHNARHALKLLKLHNDVPIVTRDDVRFAKPDPDLFLAAGQRLNVPMSRCV
                     IVGDSVWDLLAARRAFGLGVGLLSGGYGREELERAGAYRVYQDPAELLKHLDEVGVRL
                     DRRGE"
     gene            537..1007
                     /locus_tag="VFH05_14510"
     CDS             537..1007
                     /locus_tag="VFH05_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6679484.1"
                     /translation="MPKKLSAGIVFYRRGEKGIEVLLVHPGGPFWAHKDDGAWSIPKG
                     EYLEGEDPLTVAKREFYEETGTEASGPCVALTPAKQPSGKVITAWAVEGNLDPTAVRS
                     NTFSMEWPSRSGRVQSFPEVDRALWCDLALARKKLLKGQHVLLDELDQLVRSSR"
     gene            1018..1878
                     /locus_tag="VFH05_14515"
     CDS             1018..1878
                     /locus_tag="VFH05_14515"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET6679485.1"
                     /translation="MSRCLITVLLCLLSGAPLTAQAEGSDETVTAGAPVVYLDLEATT
                     WRPRGRISFGIPSVLKMKLTSAGFAVTQDPQRLHDLTVRVQYREDRGKPIGVNAYGTD
                     ISCLVLFDSPGHGVQSVAIHESPSYTDLVTAPYVEVVQKLETNPYFYFIGDIVRGWHT
                     GIDTTGALIEALTRQFDEERHRPSPTPLDTLVSPAETFPDLDAHFAPSAQENAVDELG
                     RLGDARAIEVLKKMMFEADRNMRLHAVMALGQFDAPSVTPVLTTVAQADSDRTVRDAA
                     SRVLATKTTH"
     gene            complement(2304..3158)
                     /locus_tag="VFH05_14520"
     CDS             complement(2304..3158)
                     /locus_tag="VFH05_14520"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HET6679486.1"
                     /translation="MKNLRAGRLLLIPLLLASLTFAGCVATTLLYNHADWLIARQIDG
                     YFDLTRPQKTFVSSRLTGILSHHRHEALPRYESVLHQATVRVQDGVSTDDLDWAFVQY
                     DQLRADLFGRFVSDGTEFVRQVNDSQVNRLKSALQSRLARQEALLQDDPSKRLAKRTE
                     RILGLVREWLGPLSPQQEQEITRLTMHFPDSLPAWYAHQKRRHDQLLALVESRSSDQT
                     PARLHNWLVNQDDDSDPHFAEMTKQLRRHITGLILSLDRSATPAQRQHFFAKVDDLAA
                     TIRRLQAA"
     gene            complement(3287..4159)
                     /locus_tag="VFH05_14525"
     CDS             complement(3287..4159)
                     /locus_tag="VFH05_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET6679487.1"
                     /translation="MSPRSNRRGHRRRQSRRRVTRKKLWRFVRLRSRDMRAFVGAVCT
                     AHPAVAIPVMALLLGVIWFGINWTYHATKKPSEILFPLDNAFDKNLTETWREYGPLFR
                     EHATAVITPELLAALAQVEGSGNPLARTYWRWHSSWNPLQWYRPASSAVGMYQITSGT
                     FQAAKRYCIHDHIVAEDGPWHDMRSCWFNELYTRVLPSHAIELTAALLDRQVAQAIGS
                     RSGVTPQRKQDLAAIIHLCGASAGQAYVSRGFRLLQRQQCGEHEVRAYLRKVTAAKQE
                     FARLAGRTQTATWK"
BASE COUNT          916 a         1156 c         1322 g          872 t
ORIGIN      
        1 ggcctgtttc caaaaaagaa gcggaacata ttcaagcggt gcatgcgaaa gcgttcgcag
       61 aacaggcttc ctctctccgc atcctgcctg gtgcacaaga actgctcgat acgctggccg
      121 cagaaggcgt cgctcatgcc atcgcgacga gtggacgtct gcacaatgcc cgtcatgcgt
      181 tgaagctgtt gaagctccat aacgatgtgc cgatcgtcac gcgtgacgac gtccgttttg
      241 cgaagccgga tcctgacttg tttcttgctg cgggtcagcg gctcaacgtg ccaatgagtc
      301 ggtgcgtgat cgtcggagac agcgtctggg acctgctggc ggcccgccgg gcgtttggcc
      361 ttggagtcgg gctgctctcg ggtgggtatg gacgcgagga attggaacgg gccggcgcgt
      421 atagggtgta ccaggatccg gcggaattgt tgaaacattt ggacgaagtg ggggtgaggt
      481 tggatagaag aggagagtag gcatatcaag gactcttgag gagaacgcca gcatccatgc
      541 cgaaaaagct gagcgcgggg attgtgttct atcgcagagg tgagaaaggg atcgaagtgt
      601 tgctggtcca tcccggcggg cccttctggg ctcacaaaga tgacggagcc tggtcgattc
      661 ctaaaggtga gtatctggaa ggcgaagacc cgctgaccgt tgcaaaacgg gaattctatg
      721 aggagacggg gacagaagcg agcggaccct gtgtcgcttt gaccccggca aagcagccga
      781 gcgggaaagt cattacggcc tgggcggtgg aaggaaacct cgatccgacc gcggttcgaa
      841 gcaacacatt ctcaatggag tggccgtcaa ggtctggtcg tgtccagagc tttccggaag
      901 tggaccgcgc actgtggtgc gaccttgcgt tggcgagaaa gaagctgctc aaagggcaac
      961 acgtcctgtt ggacgagctg gatcagctgg tgagatcatc gaggtgaccg tcatatcatg
     1021 agcagatgtc tgataacggt tctcctctgc ctgctatcgg gcgctccgct gacggcgcag
     1081 gccgagggga gcgatgaaac agtgaccgcc ggcgcaccgg ttgtgtactt ggacctggaa
     1141 gccacgacct ggcgtccccg cggcaggatc tcgttcggca ttccatctgt ccttaaaatg
     1201 aagctgacat cggcaggctt tgcggtgacc caggacccgc agcgcctgca tgatctgacc
     1261 gtccgggtgc aatatcgcga agaccgcggc aagccgatcg gggtcaatgc ctatgggacc
     1321 gacatcagct gtctggttct cttcgattca ccaggacatg gagtgcagtc ggtcgcgatt
     1381 cacgagtcgc cttcctacac cgatctggtg acggctccat acgtcgaagt cgtgcagaag
     1441 ttggaaacga acccgtactt ctacttcatc ggcgacatcg tccgggggtg gcacaccgga
     1501 attgacacca cgggcgcttt gattgaggcg ctcactcgtc aatttgacga ggaacgtcac
     1561 aggccgtcgc ccacgccgct cgacacattg gtctcgcctg ccgagacctt tcctgattta
     1621 gatgcgcact ttgcgccttc ggcgcaggaa aacgccgtgg acgaactcgg tcgtctcggg
     1681 gatgctcgcg cgattgaggt gttgaaaaag atgatgtttg aggccgaccg caacatgcgg
     1741 cttcatgcgg tcatggcgct gggacagttc gatgctccgt ccgtcacacc ggtcttgacc
     1801 actgtcgcgc aagccgacag cgatcgtacg gtacgggatg cggcatcccg tgtcctcgcc
     1861 acgaaaacaa cacactaacc ggtgccgagt cagggttgtg agctcactcg cgattctgaa
     1921 cggatttcaa tcagctggaa accgacgaat cgcacttgtg cttctcgcac aaaggatgag
     1981 tcgtgttgtg caattctttc gcgtagtagg aaagaatatc gcactcttct gcagacaact
     2041 cgccgctctg aagagccaag ccgatgagtc gttcacacat ccgcataaaa tctttaattt
     2101 cggttcgcat agccgcggaa atgtacaaga ccagggtttg ctccagtact gggaacaccc
     2161 ctagctctcc tgaaatagcc tcgcgggaaa aatggcagtg atggccgact agcactcggg
     2221 tgctggttta tctcgtgatt gtattcagca aggaacgaca gagaggaggt tagggcacag
     2281 gcaggatgcg cgcgagtgaa actctaggca gcttgtaaac gtcggatggt ggctgccagg
     2341 tcgtcgacct tggcgaaaaa gtgctggcgc tgtgccggcg tagcggaacg atcgagactc
     2401 aggattaagc ccgtaatgtg ccgacgcagc tgtttcgtca tctcggcgaa atgcggatcg
     2461 gagtcatcgt cctggttcac caaccagttg tgcagccgcg cgggggtctg gtcactgctg
     2521 cgagattcga ccagcgcgag caattggtca tggcggcgct tctggtgggc ataccacgca
     2581 ggcaagctat caggaaaatg catggtcagg cgagtaatct cctgctcctg ctgtggggaa
     2641 agcgggccta gccattctct caccaaaccg agaatccgct ccgtccgctt ggcgagccgt
     2701 ttgctgggat cgtcctgaag caaggcctcc tgacgtgcca gccggctctg taacgcgctc
     2761 ttgagtcggt tgacctggga gtcgttgacc tgccggacaa attctgttcc atctgacacg
     2821 aagcgcccga agagatctgc ccgtagctga tcatattgga cgaaggccca atcgaggtcg
     2881 tccgtcgaca ccccgtcctg tacccgtaca gtggcctgat gcagcacgga ttcatagcgg
     2941 ggcaaagctt catgccgatg gtgcgataaa atgccggtca gcctcgatga tacgaacgtc
     3001 ttctgggggc gagtgagatc gaagtacccg tcgatctgac gtgcgatcaa ccagtcggcg
     3061 tgattgtaga gcagtgttgt ggcaacgcag ccggcgaacg tcagcgatgc caacaagagg
     3121 ggaatgagca ggaggcgtcc tgcacgaagg ttcttcatcg gttgcgattg ccttacgatc
     3181 ggaagcggtg agtgtactga cacggtaaca caagaccctg ccccgctgct agtctctcat
     3241 cgacctggaa tttctcattc ctccgcaagt agttacaaga taatcatcac ttccacgttg
     3301 cagtttgtgt ccgtccggcc aggcgagcga attcctgttt ggcagcggtg actttccgga
     3361 ggtaggctcg tacctcgtgc tcgccgcact gctgcctttg gagcagccgg aatccgcgtg
     3421 acacgtacgc ctgtccggcc gaggctccgc acaagtgaat gatggcggca agatcctgtt
     3481 tccgctgcgg ggtgacgcct gatcgtgacc cgatcgcttg cgcgacctga cgatccaaca
     3541 gggccgccgt caactcgatg gcatgactgg gcaggacacg ggtatacagt tcattgaacc
     3601 agcaggaacg catgtcgtgc cagggcccgt cttctgccac gatatgatcg tgaatgcagt
     3661 accgcttagc ggcttgaaac gtcccggacg tgatctgata catcccgacg gcactcgagg
     3721 ctggtcggta ccattgcagg gggttccatg aggagtgcca ccgccagtag gtccgggcaa
     3781 ggggattgcc gctgccttcg acctgggcca gcgctgcaag caattcgggt gtgatgacgg
     3841 ccgttgcatg ttcacgaaag agggggccgt attcacgcca cgtctcagtg agatttttgt
     3901 cgaaggcgtt gtccaacgga aacagtattt cgctgggttt cttggtcgca tgataggtcc
     3961 agttgatccc gaaccagatc actccgagca gcagggccat gacgggaatg gcgaccgccg
     4021 gatgagcggt gcagacggcg cccacgaagg cgcgcatgtc acgcgagcgc aaacggacga
     4081 agcgccacag cttcttacgg gtaacgcggc ggcgtgactg ccggcggcga tggccgcggc
     4141 gattggaccg gggggacatg gaaacatatc atagccgaat cgtgggaaaa tggatcaagg
     4201 aatgaaacat cacccccccc cagcttgtct gtgctctcag gaaaacagag ctccagcaag
     4261 catgaa
//
LOCUS       DASSCQ010000231         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_954255, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000231 DASSCQ010000000
VERSION     DASSCQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954255"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2204)
                     /locus_tag="VFH05_14530"
     CDS             complement(<1..2204)
                     /locus_tag="VFH05_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6679488.1"
                     /translation="MSTSTPPRKPNRLVHETSPYLLQHAYNPVDWYPWGPEAFDTARQ
                     RNCPILLSIGYSACHWCHVMERESFENEAIAALMNQWFVCIKVDREERPDLDEIYMAA
                     TVAMNHGQGGWPMTVFLTPEQQPFFAGTYFPPAGRWGRPGFPDLLQQIADVWKQDPNG
                     VRSQAQDMTARLQREWQTPAPISVGEEVLDAAVDEFKQEFDARFGGFGGAPKFPPAAG
                     LSLLLRCYRRSGNPMALQMVTTTLDAMAAGGIYDHIGGGFARYSTDERWLVPHFEKML
                     YDNALLGRVYLEAFQVTGNDSYRQVVTEVLDYVLREMTDEQGAFYSATDADSEGEEGK
                     FFVWTPEQVAQALDNEEDCRRFCAVYDITKQGNWEHKNIPNRMRPLDVIAQELQVPIE
                     ELRNSVRRGRALLYEARARRVPPGLDDKIITAWNGMMISAMAEAGRVLDRPEYIEAAR
                     RSAEYLLRTHYTKDNRLLRTSRKGRAHHDAVLEDYAYLGEGLVDLYEAGGEERFLERA
                     GALAVSLLESFQDHELGGFYTTAKDHEALIVRGREGADGATPSGNAVAATFLARLSFH
                     LDRPEFREAAVAAIRAYGKQMSRYPRGFAKSLALVDFLTEGPVELALIGAKDDPAFSA
                     LRDTVRTMYLPNRILATSHRTDGVSSHPLLAGKKPVDGRTALYICRDFSCQQPLTDPV
                     SAAEVLSRLSTRSASTVGGKKLRGRMLGGSATVEGSARYAARMVNQPRTGGR"
     gene            complement(2329..2556)
                     /locus_tag="VFH05_14535"
     CDS             complement(2329..2556)
                     /locus_tag="VFH05_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679489.1"
                     /translation="MKRAFMTIMAVAVVIAFSAPSFAGEENKDKKDEKKGGHVLVYGD
                     ENKDKKEEKKGGHVDTFGEKKEDKKDEKKGK"
BASE COUNT          597 a          833 c          759 g          608 t
ORIGIN      
        1 aaccgaccac ctgtccgagg ctgattcacc attctggcgg cgtatcgtgc cgatccttcg
       61 acggtggctg aaccaccgag catccgaccc cgtagtttct tgccgcccac ggtggaagcc
      121 gagcgggtag agaggcggga aagaacctcg gccgccgaca cgggatcggt cagcggctgt
      181 tggcaggaaa aatcccggca gatgtacagc gcagttcggc catcgacggg cttctttcca
      241 gcaagcagtg ggtgtgagga gactccatca gtgcgatgac tggtggccag aatgcggttc
      301 ggcaggtaca tggtccgtac ggtgtcgcgc aaggcagaaa acgccggatc gtctttggca
      361 ccgatcaatg ccagttcgac cggtccttca gtcagaaaat caaccaatgc caagcttttg
      421 gcaaatcccc gcgggtatcg gctcatctgc ttgccatacg cacgtatggc cgcgacggcg
      481 gcctcgcgaa actcgggacg gtccaggtga aatgacagac gggctaagaa cgttgcggcc
      541 acggcattgc cgctcggcgt cgcgccatca gcaccttcac gtccgcgcac aatcagagcc
      601 tcgtgatcct ttgcggtggt gtaaaaccct ccgagctcat gatcttgaaa cgactccaga
      661 agagacaccg caagcgcacc cgccctctcg aggaaccgtt cttctccgcc ggcttcatag
      721 agatcgacca acccttctcc caaatacgca tagtcttcta agacggcgtc gtggtgcgcg
      781 cggccctttc gtgaggttcg aagcaagcga ttgtccttcg tgtaatgcgt tcgaaggaga
      841 tactcggcag accgtcgggc cgcctcgatg tactcaggtc gatccagcac acgtccggct
      901 tccgccatgg cggagatcat catgccattc caggcggtga tgatcttgtc gtccagacca
      961 ggcggcaccc gacgggcgcg ggcctcatag agtagtgctc gcccgcgtcg cacagagttc
     1021 cgcaattcct cgatcgggac ctgtaactct tgtgcaatga cgtcgagcgg ccgcatccga
     1081 ttgggaatgt tcttgtgctc ccaattgcct tgtttcgtaa tgtcgtacac cgcgcagaag
     1141 cgccggcagt cttcttcgtt gtcgagcgcc tgtgcgacct gctcgggcgt ccacacgaag
     1201 aacttgcctt cctcaccttc cgaatcggca tcggtggcgg aatagaacgc tccttgctcg
     1261 tctgtcattt cgcgaagcac gtaatcgagt acttccgtga ccacctggcg gtaggagtca
     1321 ttcccggtaa cttggaacgc ctcgagatag actctcccca gcaaagcgtt gtcgtagagc
     1381 attttctcga aatgagggac aagccagcgt tcgtcggttg aataccgtgc aaacccgcct
     1441 ccaatgtggt cgtagattcc tcctgcagcc atggcgtcga gggtggtcgt gaccatctgc
     1501 agcgccatag gattgccgct cctgcggtag catcgaagca gcagagagag tccggcagca
     1561 ggcgggaatt tgggagcacc cccgaatcca ccgaagcgtg catcaaattc ctgcttgaat
     1621 tcgtccacgg ccgcatcaag aacttcctca ccgacggaga ttggagcagg ggtttgccac
     1681 tcacgctgta atcgggcggt catgtcctga gcctgagatc ggactccgtt gggatcttgc
     1741 ttccagacgt ccgcgatctg ttgcaacaga tccggaaacc ctggacgccc ccaccgaccg
     1801 gcgggcggaa aataggttcc tgcaaagaac ggctgctgtt caggagtcag gaagacggtc
     1861 atcggccacc caccctggcc gtgattcatc gcgaccgtag cagccatata aatctcatcg
     1921 agatctggtc gttcctcgcg atcgactttg atacaaacaa accactgatt catcaacgct
     1981 gcgatcgctt cgttttcaaa cgactcacgc tccatgacat gacaccagtg acaagcggaa
     2041 taaccgatgg acaataggat cggacaattc ctctgccgtg cggtgtcaaa cgcttccggt
     2101 ccccaggggt accagtcaac cgggttatag gcgtgttgga ggagataggg actcgtctcg
     2161 tggaccaggc gattgggctt gcgtggaggt gttgaagttg acataggtca cggtagcacc
     2221 ggcttctgaa aggggtcaat gcgcccccaa aaaaaaggtc tatggtcgta agaccataga
     2281 ccttggggaa tactcgaggc ccgacaaacg tgaaggagcc gtggcggctt acttgccctt
     2341 cttctcgtcc ttcttgtctt ctttcttctc tccgaaggtg tccacgtggc cacccttctt
     2401 ctcttccttc ttgtccttat tctcatcgcc gtacacgaga acgtggccgc ccttcttttc
     2461 atccttctta tccttgttct cctcgcccgc gaacgaagga gcactgaatg caatcaccac
     2521 tgccactgcc ataatagtca taaacgcgcg cttcataggt agcccccctt agactctaca
     2581 tttgcctgac catcagacaa acgggcgcat acggtagcca atgaatcagg gatttgtcaa
     2641 tgaccacgaa aaccccgagc attgggtgca tcaatgggtg catcaatcgc ccggtcgcgt
     2701 cctctgtgat aaggttaaga gacaggattt cggatttcgg ctcatcgtcc catgaatgac
     2761 atcggtccgt actcaaacta tcgcctcacc gttcgac
//
LOCUS       DASSCQ010000232         6903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_591833, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000232 DASSCQ010000000
VERSION     DASSCQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591833"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFH05_14540"
     CDS             complement(<1..518)
                     /locus_tag="VFH05_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679490.1"
                     /translation="MNVHVGHTMGLIKRAVGTLSSQKPIRWDVPFRGLKGRPRIIILS
                     DRMDATYYATFHYPLQYLQDRALLDFAVMSGAEVRKSVSRIGEAHRLVDHLIAREAPH
                     AVIFSRYATPYGRELLEAVRSHSITTLYYCDDNLLDLPANLGNGVLAAHTDHEVVEQR
                     RACLATADRILAS"
     gene            complement(515..1414)
                     /locus_tag="VFH05_14545"
     CDS             complement(515..1414)
                     /locus_tag="VFH05_14545"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6679491.1"
                     /translation="MEPVRIFVGADRSQALAIKVLEYSIKRHTTLPVNLVPMVDLPIR
                     APKHISNSQRTGFSFSRFCIPEMAGYAGKAIYMDADMLVFKDIRSLWEIPFDGAKVVI
                     QEEVKYQELTTRKFGAPSLRRKQCAVMLLDCGRLDWKIDRIIDGLDRNEYNYEMLMYD
                     LCLVGENEVKYGVPFEWNSLEYYDQKTCLLHYTDVRTQPWTSCLNRNGEHWYNEVRLM
                     LADGSLAMDELKQEIDLGYFRPSLLWDLRIRPAIPSVLRPPFDVINTWRDVMKRYQPH
                     RDVYLAKKRRAASAKQASNPALS"
     gene            complement(1414..3867)
                     /locus_tag="VFH05_14550"
     CDS             complement(1414..3867)
                     /locus_tag="VFH05_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679492.1"
                     /translation="MRVPTFRLLMSSRRSLGGCMLLWKLHTLDYRIASVRYRALLPIH
                     ALSRRGHRSFICKSFGVDQLQGVEVVIFVKSFALKDLLFAQRASEAGIAVVLDLCDNI
                     FVPNYSVKSPICPAEVIQAMARYAAVITTTGQALADVLQRNLKVSVPVMTVFDGIEQE
                     ALSRVGRRMLRRAHWTALWRMLTSVSFWIRVTPKLRGKIAARASRWWSEHVGLSFAMT
                     PRPRIRTSATIEKSGDGSIHPLDDTAAKTLIWFGHGGGGYGRFGLSDLVDIAPDLQLL
                     AQRVKFTLIVVSNDRETYQRLVKPLPIDTRYVEWDSESIRQLIRTSDLTIVPNSRDPF
                     AVCKSANRAVLSLSLGVPVVASRTPAMEVLEGCVMFDDWVEGSYRYLTDQKLVTEHLK
                     QAQRVLEKEFGLDRIAAQWEDVLDRVRRSKVQRSGKGARQPRSTVAVVIHLMQDLDLA
                     LPMLREITRSQHLRAQAWVSLSVFEASPRVWKGLRRHDLDFRILDDQTDVDLGPIRSR
                     DIGAVLTVAETNQSPHRFPHRIASLANEMGIATYTMQHGYENIGLTYTDKRYPIEQIV
                     FASKTIFTWGHPTLLHPRVSETTRAKCIPVGCCKVVPSEVIRLPVPVNGARVIGIFEN
                     LHWDRYDRSYAERFILDTQSLAFRHPEILFLVKPHHAGQWLTSRYRGGVPSAPNLIIA
                     DPAHPDWEQFTASQLINSLDGVITTPSTVALDAARAGKPVAVVGYGLDLSVYRPLPVL
                     TGSEDWMAFLHNITDDGRKRVLVDQACEFVTRTIHPGVAAANILRKIEDDLHVQSTGW
                     GRTGERADPRASRQLQKVV"
     gene            complement(3885..4646)
                     /locus_tag="VFH05_14555"
     CDS             complement(3885..4646)
                     /locus_tag="VFH05_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6679493.1"
                     /translation="MNVILTIARNTFKEVLRDKVFYNLFVFALLMIGSALLLGTLTIG
                     EQSKIIKDIGLASIDLFGVLIAIFVGVGLISKEIEKRTIYTIIAKPVHRYQFLLGRFA
                     GLVFTMWVNTAVMLVAFCVILVVGGTSPDSGLMKAVGLMTVAQLIVLSAAMLFSTFTT
                     PTLSGIFTLALYVIGELTPDLKTLSEKLSGVSRGLLSGSYYLLPNLALFNVKGEAVHG
                     VPITAGYMLTAIGYGVSYAAVVLLLSCFVFQRRDF"
     gene            complement(4643..5584)
                     /locus_tag="VFH05_14560"
     CDS             complement(4643..5584)
                     /locus_tag="VFH05_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6679494.1"
                     /translation="MIPLQVHALTKIYQPAWSRKQFVAVDRLNFEVRKGEIFGFLGHN
                     GSGKTTTIKMILGLVRPTAGRALILGRDFRDIEVKRSVGYLPESPYFYDYLTAEEFLK
                     FYGRLFNMGGSELACKIDSLLEQVGLRDARSVQLRKFSKGMLQRVGIAQALINDPLLV
                     ILDEPMSGLDPIGRRDVRDIILRLREQGKTVFFSSHILPDVEMICDRVGILAKGELKA
                     IGPVRDLIGSENENDHRVEIQVAGATEGLWEKANRLGGAVNEQGGVTLITMANQRSAE
                     ELLGYIQSDKGRLVSFAPHKRSLEDVFLEETGEGIRS"
     gene            complement(5581..6462)
                     /locus_tag="VFH05_14565"
     CDS             complement(5581..6462)
                     /locus_tag="VFH05_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679495.1"
                     /translation="MSLRYAYRMTPWFLGFAVALAAHAMHLESDQARASMPAMQRFTL
                     LPDGETLKAMSLGFKDVVADIIWLQAIQAIGERRVSDDAGQWIYRAMDIVTTLDTRFI
                     EAYEAGGTALCTVVVMPEASNRLLEKGMQYNPGEWRLPFMLGVNYYFELADDANAAKY
                     IGRAAEIGGGPSYLAAFASRLYMTAREPQKAIEFLAKSYEQTTDPHMKEFLELRLKHA
                     VVERDLQLLETAIESYRRQFGTTPATLESLVKAGVLREVPVDPFGDRYIYDDKTLNVR
                     SKSVDGRFKADGHRRQR"
     gene            complement(6459..>6903)
                     /locus_tag="VFH05_14570"
     CDS             complement(6459..>6903)
                     /locus_tag="VFH05_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679496.1"
                     /translation="STLNALDGRYPYRLGTLYQLLAETAETSQEKSARRLAEATDSFR
                     QAVIRDPYFPPSRVALGKIQRLEGELRQARTSFSEALELEPNYLPARALRIQVDMELG
                     DSTLARDEYREMLTIAERWRGKATTALEMQFLDVDWDQLKRLVQS"
BASE COUNT         1528 a         2013 c         1851 g         1511 t
ORIGIN      
        1 gaagcaagta tgcgatctgc agtggcaagg cacgctcttc gttgttcgac cacctcgtga
       61 tccgtatgtg ctgcgagcac gccattgccg aggttcgcag ggaggtccaa cagattatcg
      121 tcgcaataat acaacgtcgt gatgctgtga gaccgaacgg cttccaatag ctcgcgtccg
      181 tacggagtcg cgtaccgact gaagatgacg gcatgaggag cttcgcgagc gatcagatgg
      241 tcaacaagcc ggtgagcttc tcctatccgt gatacagatt tcctgacttc agcgccggac
      301 atgactgcaa aatccagcaa cgctctgtct tgaaggtact ggagaggata atgaaatgtg
      361 gcgtaatagg tggcatccat ccgatcgctc aggatgatga ttcttggcct tcctttcagg
      421 ccgcgaaacg gtacgtccca gcgaatcggt ttctgcgagc tgagcgtccc caccgctctc
      481 ttaatgagac ccatcgtatg gccgacgtgc acattcatga cagggcaggg ttcgaggcct
      541 gtttcgcgga cgctgcccgt cgctttttcg caaggtagac gtcacgatgc ggctgatacc
      601 gtttcatgac atcgcgccat gtgttgatca cgtcgaacgg cggacgtagg acggatggaa
      661 tggcgggcct gatacgaagg tcccataaga gcgacggacg aaagtacccc aagtcgattt
      721 cctgcttgag ttcatccatc gctaaggatc cgtctgcaag catgagccgg acttcgttat
      781 accagtgctc tccattcctg ttgaggcaag aggtccaggg ctgggtcctg acgtcagtgt
      841 aatggagcag gcacgtcttc tggtcatagt attccaggct gttccattcg aacggcacgc
      901 cgtacttgac ttcgttttcc cccacgaggc acaagtcgta catcaacatc tcatagttat
      961 actcattcct atccaatcca tcgatgatgc ggtcgatctt ccagtcgaga cggccgcaat
     1021 caagcagcat gacggcacac tgcttccttc tcaaagaggg cgcaccaaat ttcctcgtcg
     1081 tgagttcctg atatttcact tcttcttgaa tcaccacctt ggccccgtcg aacggaatct
     1141 cccataagga gcggatgtcc ttgaacacca gcatgtcggc gtccatgtag atggcttttc
     1201 ccgcataacc tgccatttcc ggaatgcaga atctgctgaa tgaaaatcca gtccgctggc
     1261 tgttgctgat gtgcttcggt gcacgaatgg gcaaatcaac catcggtact aggttgacag
     1321 gcaacgtggt atggcgcttg atcgaatatt ccaaaacctt aatcgccagc gcctgggacc
     1381 gatcagcacc gacaaatatt ctcaccggtt ccattacacc accttctgta gctggcgaga
     1441 ggcccttggg tcagcacgct ctccggttcg gccccagcct gtgctttgga catgtaagtc
     1501 atcctcgatc ttgcgcaaaa tgttggcggc agcaacacct gggtgtatgg tgcgcgtgac
     1561 aaactcacag gcctggtcga ccaacacacg ctttctccca tcgtccgtta tgttgtgcaa
     1621 aaaggccatc caatcctcac ttcctgtcag gacaggaagc ggccgataga ccgagagatc
     1681 aagaccgtat ccgacgacgg caaccggctt tcccgccctg gccgcgtcca aggcaacggt
     1741 cgaaggagtc gtgatgacac catcaagcga attgatgagt tgggaagcag tgaactgttc
     1801 ccagtccgga tgagccggat ctgcaatgat aagatttggc gcactgggca ccccgccccg
     1861 gtaccgcgag gtcagccatt gtcccgcatg gtgaggcttc accaggaaaa gaatttctgg
     1921 gtggcggaag gccaaagact gtgtgtccag aatgaagcgc tcggcatagc ttctgtcata
     1981 gcggtcccaa tgcaagtttt cgaatatgcc gatgacgcga gcaccgttca ccggcaccgg
     2041 cagtcttatg acctcggacg ggaccacctt gcagcaacca accggaatac attttgcgcg
     2101 agtagtctcg gagactctgg gatgcaggag ggtaggatgg ccccatgtaa agatcgtctt
     2161 cgaagcgaag acaatctgct cgatcggata tcgcttgtct gtataggtca ggccgatatt
     2221 ttcgtacccg tgctgcatgg tgtaagtggc gatccccatc tcattggcaa gactggcgat
     2281 acggtgcggg aaccgatgcg gtgactggtt ggtctccgcg acggtaagca ccgctccaat
     2341 gtcccgagat ctgatcggtc ccagatctac gtccgtctga tcgtccagga tgcggaagtc
     2401 gagatcgtgc ctgcgtaatc ctttccacac ccggggcgag gcctcgaaca ccgataagga
     2461 gacccacgcc tgagcgcgta aatgctgcga gcgtgtgatt tccctgagca tcggtagcgc
     2521 cagatcgaga tcctgcatca gatggatcac cacagccacc gttgatcgtg gttgacgtgc
     2581 ccctttcccc gatcgttgaa ccttcgagcg gcggactcga tccagcacgt cctcccactg
     2641 agcggctatg cgatcgagtc caaattcttt ttcgagcacc cgttgtgctt gtttcagatg
     2701 ctccgtcacc aatttctggt ctgtgagata acggtatgaa ccctccaccc agtcatcgaa
     2761 catgacgcag ccctcaagca cctccatggc cggcgtacgg ctggccacga ccggtactcc
     2821 caggctcaat gagagaacgg cccggttggc cgatttgcaa acggcgaaag gatctctcga
     2881 gttggggacg atcgtgagat cgctcgttct gatcagttgc cgtatcgatt cgctgtccca
     2941 ttcgacgtaa cgggtgtcga tgggaagagg tttcaccaga cgctgatacg tctcccgatc
     3001 gttactcacc acgatgagtg taaacttgac ccgttgggcg aggagctgaa ggtcgggagc
     3061 gatatccacc agatcggaga ggccgaatcg cccataaccg ccgcccccgt gaccgaacca
     3121 gatgagcgtc ttggcagcag tgtcgtccaa tgggtggatg gagccgtccc cgcttttctc
     3181 gatcgtggca ctggtgcgaa ttctgggtcg tggagtcatg gcgaaggaaa gtccgacgtg
     3241 ctcggaccac caccgactcg cccgagcggc tattttcccc ctcagcttcg gcgtcacacg
     3301 gatccagaag ctgacactgg tcagcatccg ccacaatgcg gtccagtgcg cccgcctgag
     3361 catccggcgt ccgacgcggg acagtgcctc ctgctcaatt ccatcgaaca cggtcatgac
     3421 cggcacggac accttgaggt ttctctgcag cacgtccgca agagcctggc cggttgtggt
     3481 gatgacggcc gcatatcggg ccatggcctg aatgacttcg gcgggacaga tcggcgactt
     3541 cacactatag ttgggcacaa agatgttgtc gcaaagatcc agcacgacgg cgatccctgc
     3601 ctcgctggcc cgttgcgcaa acagcaggtc tttcaacgcg aacgatttta cgaaaatgac
     3661 cacctccacg ccttgcaact gatcgacgcc aaatgatttg caaatgaagg accggtgtcc
     3721 gcggcgggat aatgcgtgga tgggaaggag ggcgcgataa cggacactcg cgattcgata
     3781 atcaagcgta tggagtttcc acaggagcat gcatcccccc agagaccgcc gactgctcat
     3841 cagcagccgg aacgtcggca cacgcattgc gagttcgtga tgcgctagaa gtcgcgccgc
     3901 tgaaatacga agcaggacag gagcaacacg acagcggcgt acgatacgcc atagccaatg
     3961 gcggtcagca tatacccggc cgtaatcgga acgccgtgaa ccgcttctcc tttgacgttg
     4021 aataacgcga ggttcggaag cagatagtaa ctcccgctca gcagtcctcg agacacaccg
     4081 ctcagcttct cactcaacgt cttgaggtcc ggcgtcagtt ctccaatcac gtacagtgct
     4141 aaggtaaaga tgccgctcaa ggtaggggtc gtaaaggtgg agaagagcat ggcggccgac
     4201 aacacgatga gttgggcaac cgtcatgagg cctacggcct tcatcaagcc gctgtccggc
     4261 gaagtgccgc ccacgaccag aatcacgcag aacgcgacca acatgaccgc cgtattcacc
     4321 cacatcgtga agacaagtcc ggcgaaccgc cccagcaaaa attgataccg gtggaccggc
     4381 ttggcgatga tcgtgtagat ggtgcgtttt tcgatttcct tgctgatcaa cccgaccccg
     4441 acgaagatcg cgatcaacac gccgaagagg tcaatactgg ctaatccgat atccttgatg
     4501 atcttgcttt gttctccgat ggtcaacgtg ccaagaagta acgcgctacc aatcatcagc
     4561 aacgcgaaga cgaacaagtt gtagaacacc ttgtcccgga gaacctcttt aaaggtattc
     4621 cttgcgatcg tcaggatgac gttcatgacc gaatcccttc gcccgtctcc tcgagaaata
     4681 catcctcaag ggaacgctta tgcggcgcaa acgagacgag gcggcccttg tctgattgga
     4741 tgtatccaag cagttcctcc gccgaccgct gattggccat cgtgatgagt gtcactccgc
     4801 cctgttcatt caccgctcct ccgagacgat tcgctttttc ccagagtcct tcagtcgcac
     4861 cggccacctg aatctccacc cgatggtcgt tctcgttctc gctgccgatc aagtccctga
     4921 cgggtccgat cgccttcagt tcgccttttg caaggattcc gacacgatca cagatcatct
     4981 cgacgtccgg cagtatgtga gaactgaaaa agaccgtctt cccttgttct ctcagacgaa
     5041 gaatgatgtc gcggacatcc cggcgtccaa tcggatcgag cccggacatg ggctcatcca
     5101 ggatcacgag cagaggatcg ttgatcagag cttgggcgat accgacacgc tgcagcattc
     5161 ccttcgagaa ctttcggagt tgaacacttc gcgcatcccg caagcccacc tgttcgagca
     5221 acgagtcaat cttgcaagcc agttcgcttc cacccatgtt gaacagccgg ccataaaatt
     5281 tgagaaattc ttctgcggtc aaatagtcat aaaaatacgg agactccggc agatatccca
     5341 ccgatcgctt cacctcgatg tcgcggaaat cccttcccaa tatcagcgca cggcctgcgg
     5401 tcggacgaac gagcccgaga atcatcttga tcgtcgtcgt ctttcccgaa ccgttatgtc
     5461 cgaggaagcc gaagatctca cctttccgta cctcaaagtt cagtcgatcg actgccacaa
     5521 actgctttcg cgaccaagcc ggctgataaa tcttggtaag cgcgtggacc tgtagtggga
     5581 tcatcgctgc ctccggtgac cgtctgcctt gaatcggcca tcgacggatt tgctgcgcac
     5641 gttcagcgtc ttatcatcgt agatgtaacg gtctccgaac ggatcaactg gcacttccct
     5701 cagcactccg gccttgacca gcgactctaa tgtggcggga gttgtcccga attgccgtcg
     5761 atagctctcg atcgccgttt ctaacaattg tagatcccgc tccacgacag catgtttcaa
     5821 tcgcaactcg aggaactcct tcatgtgagg atctgtcgtc tgctcgtacg acttcgcaag
     5881 aaactcgatc gctttttgcg gctctctggc ggtcatgtac agacgcgatg caaaggccgc
     5941 aaggtagctc ggcccccctc cgatctcggc agctcgaccg atatatttcg cagcgttggc
     6001 gtcatcggcc aattcgaagt agtaattgac cccgagcatg aagggcagcc tccattctcc
     6061 ggggttgtat tgcatcccct tttcgaggag gcgattgctc gcttcaggca tgaccacgac
     6121 ggtgcagagc gctgtccctc ctgcctcata cgcttcaatg aatctcgtat cgagggtggt
     6181 gacgatatcc atggcacgat aaatccattg ccctgcgtcg tccgagacgc gacgttctcc
     6241 aatcgcctgt atcgcttgaa gccatatgat atctgccacc acgtccttga aacccaacga
     6301 catggccttg agcgtctccc catcgggcag gagcgtgaac cgttgcattg cgggcatgct
     6361 cgcccgggcc tgatccgact ccaggtgcat ggcatgggcc gcgagggcca ccgcgaagcc
     6421 gaggaaccag ggtgtcattc tgtaggcata gcgcaagctc atgactgcac cagccgcttc
     6481 aattgatccc aatcaacatc gagaaattgc atctcgagag cggttgtggc tttccctctc
     6541 catcgctcgg cgatcgtaag catctcccga tattcgtcac gggcaagggt ggaatccccg
     6601 agttccatat ccacttggat gcgaagcgct cgtgcgggca agtaattcgg ttcaagttcc
     6661 agcgcttctg agaatgacgt ccgggcctgc cggagttcgc cttctagccg ctgaattttc
     6721 cccagggcca cccgactggg aggaaaatac ggatctctga tcactgcttg ccggaaggag
     6781 tcggtcgcct ctgccaatcg acgcgcgctc ttctcctgag atgtctcggc ggtttcagcg
     6841 agcaactgat agagcgtgcc caggcgatag ggatatctgc cgtccagggc gttgagtgtg
     6901 gaa
//
LOCUS       DASSCQ010000233        33095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_152270, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000233 DASSCQ010000000
VERSION     DASSCQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 33095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33095
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152270"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1665)
                     /gene="guaA"
                     /locus_tag="VFH05_14575"
     CDS             complement(109..1665)
                     /gene="guaA"
                     /locus_tag="VFH05_14575"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET6679497.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVPLATI
                     LAYRPQGIVLSGGPSSVYEKKAPAVPKELFDQGIPILGICYGMQLVTHLSGGEVAKSK
                     HREYGRAELRIDDASDLFAGIGKDRSTVVWMSHGDRIERMPPGFRSIAHTENSPVAAM
                     KRVDGKRRIYCLQFHPEVAHTPEGTQLLRNFVYQICGSKPTWTMQSYVETAVKQIREQ
                     VGGERVICALSGGVDSSVAAALTHRAIGDQLTCIFVDNGVLRAGERDQVQKTFASQLH
                     LNLRILDRTGAFLAGLKNVTDPERKRKAIGRLFIRNFETEARKLKDVRYLVQGTLYPD
                     VIESISFKGPSATIKTHHNVGGLPARMRLKLIEPLRELFKDEVRVLGKELGLPDEIIW
                     RQPFPGPGLAIRVLGSVTKERLAILRGAETIVDQEIRSAGLYRDIWQAFAVLLPIRTV
                     GVMGDQRTYENVVAIRAVTSLDGMTADWAKIPNEVLGRMSNRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            complement(1780..3246)
                     /gene="guaB"
                     /locus_tag="VFH05_14580"
     CDS             complement(1780..3246)
                     /gene="guaB"
                     /locus_tag="VFH05_14580"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET6679498.1"
                     /translation="MLEKEPRLGLTYDDVVLVPSKSAVLPSEVDVRTQLTRNIQINIP
                     IISSAMDTVTESRLAIAMAREGGVGIIHRVLTPADQAAEVDRVKKSESGMILDPVTIS
                     PDQTIRDAHELMARYRISGIPVTKSKKLVGILTNRDLRFETRLDLKVSQVMKRDKLVT
                     APEGTSLEKAREILHEHRIEKLPVVNKHGDLKGLITIKDIEKRIKYPNACKDAHGRLR
                     VGAAVGVGPDIEDRVTQLKKAGVDLLVVDTAHGHAQAVLETVRMIKKQHPSLELIAGN
                     IATAEAAKDLLRAGVDAVKVGVGPGSICTTRIVSGSGMPQLTAIADCAKVLEGSGVPI
                     VADGGIKFSGDISKALAAGASVVMLGGLLAGTEESPGETVLYQARTYKVYRGMGSIGA
                     MERGGGDRYGQSGRPAQKLVPEGIEGRVPYKGPLSAVIYQLVGGVKSGMGYCGCKTIP
                     ELQKNASFIRQTVAGLRESHVHDVIITKEAPNYRMDWE"
     gene            complement(3268..6237)
                     /locus_tag="VFH05_14585"
     CDS             complement(3268..6237)
                     /locus_tag="VFH05_14585"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HET6679499.1"
                     /translation="MIKAWLLDEMFRFDRAPLTVEGSHGEWGAGDSIIEEEELPPAPA
                     NVAAKCGNGRITITWDPVPDALYYNLYFQTTKGVHIKFSELTRPIAGPEDFKTVIGVK
                     KEKATCLEGAQSPFLHDDLANGTCYHYVVTVVTAKGESLESQEVMAIPSPYLLAACIG
                     QEGVDDGEFSSPTGIALDKDGNLYVADTDNHSIQKFDKAGHFIARWGGEPSSQEGSFY
                     YPRGLAVGANDSLYIADSGNNRVQKFDLDGNVMQAWGKFGFAWRGADMGKFDVPWGLA
                     TDQDGNLYVSDTSNARIQKFKADGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNIYVAD
                     EGNNRVQKFDARGSFLTKWGREGAGPGQFKAPWGIACDALGNVYVVDTGNHRIQKFDG
                     NGTFLCAWGNRGKSEGQLNFPYGIAVDKEGCVYVVDSGNNRILKYVPTDEEINRGKEE
                     KPKSQGTNSMPPPSNVAVKAGDTETFLSWIEVPGAVSYNLYFNTSPLLTTQSGTKIEG
                     VTNPFTHTGLTNDTPYYYAITAMFDGEVESELSHVVTATPVLIDITAPQNPYAVINHG
                     AFMTNSPEVVVTISANDIDTGVGAYYVSEAPMTPMAGTPGWVEVTPSLKFGATIPFIL
                     SPGDGQKTIFVWFKDVGGNVSTPASATILVNTSGYLCVSRWGRSGRGASLLHGGEFMA
                     PIYGLCIDQQGSLFVVDNGNNRIQKFDNAGNFIILWGNFGSANSNFHNPTGIACDGKG
                     DVWVVDTNNHRVQKFDGKLGGYLMKFGSRGNGEGQFNSPWGIAVDRVRGYVYVVDSAN
                     FRVQKFDMTGEFIMSWGSFGNGDGQFYFPRGIAVDQSDGAVYVVDMGNHRVQKFDTST
                     NVLPQLLTKWGGSVEAGHASSPLSQEAGQLRSPWGIAVDGAGDVYVSDTGNHRIEKFD
                     KEGNFITQWGGYGNGDGQFNFPYGIAVDAKGSVFAVDSGNTRVQQFMPADEGSERLQE
                     EAEAAAEIEKAQQPQKA"
     gene            complement(6717..7517)
                     /gene="scpB"
                     /locus_tag="VFH05_14590"
     CDS             complement(6717..7517)
                     /gene="scpB"
                     /locus_tag="VFH05_14590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET6679500.1"
                     /translation="MDSAALQDAAIVSADEAVNGNGALDSAASESGGAQLEPQEEDLK
                     GTELIGVLEALLFVSTEPVPVSRLATVIGTVSKSEVEQALKALEQELAREGRGIQLVK
                     LAGGYRLVTKSDYAPWLKRLDKAKAAQKLSRSALESLAIIAYKQPLVRAEIEDIRGVE
                     TSGVLRTLLERKLVRIVGRKEVPGRPIMYGTTKFFLEHFGLQDITQLPPLREFKELGE
                     AEQALLPMEQEPMPQEDVAAAASEPALSTDPELAWESQSLIPEAVEQS"
     gene            complement(7520..8317)
                     /locus_tag="VFH05_14595"
     CDS             complement(7520..8317)
                     /locus_tag="VFH05_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET6679501.1"
                     /translation="MDNQLDGAEQTEFAYQVRIENFEGPLDLLLHLIKKNEINIYDIP
                     IALIAQQYLSYIEAMKELNLAVAGEFLVMAATLLQIKSKMLLPVEETAEDEDDGPDPR
                     EELVRRLLEYKTFKEAARQLDTQEKMWREIYSRPAAPHEEAPDTDEAMLENIGLFDLV
                     DALQGILNRNPGKKLLEILPDNLTVRDRMNAILEALEGQESIGFEALFEASCHRLLII
                     VTFLALLELIRLRTVRVYQAESFGPILVSRSFSLVPDPAELDDSEWR"
     gene            complement(8376..9431)
                     /locus_tag="VFH05_14600"
     CDS             complement(8376..9431)
                     /locus_tag="VFH05_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET6679502.1"
                     /translation="MTRPGRAVAILLVLLYCFADYAAAECVVEQAVAGSQRLFKTHLS
                     AGCTPVEREARAVDASAVLAALKRGQTIDLAGVVIQGDVALDALPVTESPPVIDDIID
                     RDDNEIRVIGAPVAIVDSVVRGTVTHRTAVGTLVFSGPVTFVGTTFERMVDLSRSVFT
                     QPVAWSGAVFLREGYFVQCHFLRGVTGEKTAFGSHTRFHRSQFHGPVTLQQSGFSGMA
                     EFLEVRFDQDANLSRTYFKRGTGFSGSHFKQLADFSETLFDGDAFFTFTRFDGDAFFR
                     RATFRATADFDDAQFNAREDFSKTFFEKGPQFTRARRSATHPDSLGIENRQVQYVITL
                     SLLLFSAVLIAYLVRSR"
     gene            9523..10167
                     /locus_tag="VFH05_14605"
     CDS             9523..10167
                     /locus_tag="VFH05_14605"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET6679503.1"
                     /translation="MLMRTLIAGSLLVGSVVLTTSASYALDYVELPVLAAVRSNNLGV
                     FEIMLMGWDKKADPNAVQLQVYIAGVRYGKHHLGSMVQAFDYAVERTPAVLHTGTVSV
                     QGVTYLPTTSDGPSAGAAMAVGFIAVFKGDRIQRGTALTGTLEPGGHIGRVGSLPDKI
                     RAAKREGYHTVLVPRGQLHTSEWNLIELGFQLNISVKEVDTVDEAYQFMTGNRL"
     gene            complement(10208..10678)
                     /locus_tag="VFH05_14610"
     CDS             complement(10208..10678)
                     /locus_tag="VFH05_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679504.1"
                     /translation="MSHFLFIAAALRERTSEESAELQLSHGLWGLCTALIRTNLQTYL
                     TPDSCGLVYVLKAGLCAEFTIVPPPLDFAKLDEFLKDDLRTEARFGFIRIQGIKRWAW
                     TPQASQALLQHVLKIPDQAELARRLALGMHRLTAEEFTAIVEGIRSGTGSGHKT"
     gene            complement(10778..11203)
                     /locus_tag="VFH05_14615"
     CDS             complement(10778..11203)
                     /locus_tag="VFH05_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679505.1"
                     /translation="MAKMKKRPAKKHTAKTKTKKSAKKPVRKPGKKQSVAKKATPAKK
                     AVGVPVPSKPAVAKKAGPDEELRKTTAKGAPAMAPKEPDLDEEEMGEEEIDLTGESES
                     ESEIDEEMEEDLSLDDDEEDVDYLEKSDDLLDDSDDYRH"
     gene            complement(11369..12346)
                     /locus_tag="VFH05_14620"
     CDS             complement(11369..12346)
                     /locus_tag="VFH05_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679506.1"
                     /translation="MCRSIAIVLFLVGLVDGHSVWALTLTSPSQGATLQAGRLVTVEV
                     SVGKEFNLRTIHYYWYRPDEEPLASNQAHPAPFKPIRGESPFAGTVLVPSDALGTMRL
                     LAVGEVVRGRLESHEEFDEVLLTVEATAELQTIEFAVEKPWKMQQVGRRMVVPAIGQF
                     SDGVWRPLSGSSTGSRFHSSDERVVSVDATGVLHVIGRGKAIVTVENGGKTGAVEVIV
                     QSDEESNRSPIAEVVKELHARSGELVLLDGLRSRDPDGDPLHFEWKQILGHRVMLSNV
                     NEAKATFVAPKVSERKRYQFSLVVTDMAGPDVMKGADSVPAVVTVWITP"
     gene            12486..12872
                     /locus_tag="VFH05_14625"
     CDS             12486..12872
                     /locus_tag="VFH05_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679507.1"
                     /translation="MTIAFFRRTLVAMLTMLSLTAWPTTATPPYTAIIIDSGSPYFVP
                     KSATVSTGAPIRWENPTPTEHTVTHVGCLEDGAGCAFDSGLVLPNDSYTHPGLAPGKY
                     QYICRIHPIMHGTIIVTDSAPAPSQL"
     gene            12943..14433
                     /locus_tag="VFH05_14630"
     CDS             12943..14433
                     /locus_tag="VFH05_14630"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6679508.1"
                     /translation="MKPAVAVHEPRNLTGETLNLLAWHIPDLIAYQHHADEWWFAPLD
                     DNLPIVRVNRIGLEMLKAMNGHTTVRALIDRYGSRICGPDGQPGQWHLERWALPNFSL
                     CYYGTEPPGGHRHQAKWDVLLQQIREGWSGQEGFEGEERLQDFHVHDLKDSPEDDEHF
                     DLIETTVSHLFREPNEALNGLTYGRLVMQKLRRLGWFNPKPQVIVEVGGGLGYVAREL
                     GKELLPFEKQGITYCSVDITAPFLQLQLSRARAGGWQCAGAQANGEWLPFKDHSVDLV
                     IDNENMADMTPVKLTKQELLTGTGATPPHQEALDWIRRVRLPIGKDLPEDVIFNLGPF
                     RFVSELWRVLKPGGRAFLTEFGIEEGWPAPVKLPGHTEYEVQYSHLRQAVRWLGFQEQ
                     YLSLPQFLAMKPDTKVLCTGAAYTIQRFCQAMNKPFVVRAYTEQELSHALGDLLPKFQ
                     GTHYHDVADPAWFGLVDFKVLLLEKPGGAPKPTFTETKGYRWYSQR"
     gene            complement(14575..15507)
                     /locus_tag="VFH05_14635"
     CDS             complement(14575..15507)
                     /locus_tag="VFH05_14635"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET6679509.1"
                     /translation="MTSKRLAGIDIGTLTCRLLIADLPANNRLNELCSERRILRLGEG
                     VDQSKRLKHEAVERVIHCLKDWRATIESYHVHAHVAVATSAVRDAANKQEFLNRVRSE
                     AGFEVEILSGDEEARRTMLGIHSGLPGDIKDVLALDIGGGSTEFILTLQGQSPVVRSI
                     DIGVVRLSERLLHHDPPTRQEIAEARAWIQRETTVVLAAMPQRTGLTLVGTAGTITSL
                     AAMALQLPAYDAARIHNYRLALNTITDLEKHLLSRSKAARIGMPGLERNREDVIAAGA
                     IILRTVMDILGERECLVSDRGLREGVLIELATRH"
     gene            complement(15504..15827)
                     /locus_tag="VFH05_14640"
     CDS             complement(15504..15827)
                     /locus_tag="VFH05_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679510.1"
                     /translation="MGLLQRIKHDLKTGLTTLRLGTAQAANRALEESELLRLRLEVRR
                     IDQQLKELYRDVGERAVTLREAGEPVERVLYDTEIARLVKDIHQLKATSGKLEAEMQE
                     IRDAE"
     gene            complement(16060..18597)
                     /gene="recG"
                     /locus_tag="VFH05_14645"
     CDS             complement(16060..18597)
                     /gene="recG"
                     /locus_tag="VFH05_14645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET6679511.1"
                     /translation="MVVHRSAEAMAQFQQWLDRLARPIEFASRDAFARLPTVKNLSTY
                     VSSQILHALSHQVYPKSIEAALQKLRALFEEDQQRFTLEEQQRRLREAAGILRTLREA
                     TEDQGRVWEEPEALTVTQPAPSKSTRDLWTVPIRFAKGVGPQRASTLQRLGIETVEDA
                     LWTVPWRYEDRSVMTPIGQLVPGMVTAVCGTVVKNSAKRTRNRRFTVLEIGLEDQTGR
                     LQAVFFNQPYLEQIFAVGKSIMMSGRVIAGRQGWMVPRMDVSQYEVVGEGTESTLHVG
                     RIVPIYHETKGWTSRQMRVLMRGLVDEHAADLQDILPVPIRARQRLLPVQEAVVQIHF
                     PKAGADLAALELGKSPVHRRLAFEELFLLQMALAARQRSVQVEKKRLHFNPRTPLLDK
                     LGRVLPFTLTTAQDQVIREIFRDMIAPRPMNRLVQGDVGSGKTVVALHAIVLACGSGY
                     QAALMAPTEILAEQHYRNLSPLLHSLGVNAALLRGSDKAATKKNQIAHLASGRIQVAI
                     GTHALIQKGVAFKSLGLAVVDEQHKFGVLQRKTLIEKGYGPDVVVLTATPIPRTLAMT
                     VYGDLDVSIIHTLPPGRKPVRTFLFGEAQRRRAYQILRDELQNKRQVYVVYPLVEESE
                     KTDLQAAIQGAERLQEGELKEFRVGVLHGRMKQADKEAVMTDFTEGKIQVLVATTVIE
                     VGVDVPNATVMMIEHAERFGLAQLHQLRGRVGRAGYQSYCLLMAATMGRNKTLPGKRP
                     TGNQESASLVKERLEALVRSNDGFVIAEEDLRIRGPGEFFGFRQWGMPEFRVANMVRD
                     GDLLHQARLEAFSLLKQDPQLTASAHQGIKEAMLRKWQKKLELGSIS"
     gene            19015..20451
                     /locus_tag="VFH05_14650"
     CDS             19015..20451
                     /locus_tag="VFH05_14650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6679512.1"
                     /translation="MYRRNISNVLYPIVAGLGLVIVYQSIVKPTYISPLTASHREQGP
                     LETGTVSREPLLEPMIEEPHATAPKQLRVLEAPAVPESKHRGSLETILEEIEKGNLKE
                     AEASLIHLPTTLTDDSRIYAAVLWNNLGIEQEKLEGTKGSLKTFKKAASLDPKNPTIQ
                     LNLAHAYWEQRDPAMSQDFLERLIALAPQEPFPHVALAELLHERGRLSEAARHLDQAA
                     ERVARDPSMQSYLQTVSSKVRRTDEMESRLSTREGAHFTVKYDGETDQATWTVVLDVL
                     EEAYREIGQKFGHFPAKTIVVVLHPRATFQGATGSPAWADGLFDPVLGRIHVPTQSAL
                     EDRAWLRRVLRHEFVHALLHDQQGLNHHAIPTWLNEGLAMQLSNDHWTDLDQLHQQKL
                     SIIPLTALEGSWGELQSEEASVAYLEADSAAGYLITRYGMHEVQQLLARLKQKQSLPA
                     AIHAQLSLSYEQLQSRWIAHLQERRPRG"
     gene            complement(20339..21256)
                     /locus_tag="VFH05_14655"
     CDS             complement(20339..21256)
                     /locus_tag="VFH05_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679513.1"
                     /translation="MRSISRLSIRTCLQHLYSLAIVLTFFEVGTVPATAGTTDSPSMA
                     AGHIRTVVAAGFGFQDDETSSITVKTYDAQSGEVLTADTYELDIKDDGPPTSHPKTRI
                     FAGGVGLGAGSLSEFTLRVYDAANGHFLWEGRLNLTIGDHPEAATHQVMAHVQPRAAV
                     TRIASSTKGTGQPYFVLRARHPDTGQLMWSDEFSTEASTVRIERIGRSVIGVTGAAPR
                     DIDFRIMMPDQAGRRLLWEDKVVPEEEIEQSAVPDRSDETAGKTPQGGRSPIPSVVSP
                     ADALSNGIEAARRTRTVEHESPRASFAFA"
     gene            complement(21354..21758)
                     /locus_tag="VFH05_14660"
     CDS             complement(21354..21758)
                     /locus_tag="VFH05_14660"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HET6679514.1"
                     /translation="MTEPFSKQSGQCVLVYDGQCRLCVTAKNGLEQLRRESHADEVRL
                     IPYQSEEAKQVLGAHYRPGRPPAAFLVHPDRTIATGLDAFLPLLPGLKGGHPLAKFLS
                     LRLVKPFAYLAYRLVARYRYRLFGEVPLKRLD"
     gene            complement(22105..23172)
                     /locus_tag="VFH05_14665"
     CDS             complement(22105..23172)
                     /locus_tag="VFH05_14665"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET6679515.1"
                     /translation="MRQPITTFDEFRDALSAYRLPRVILAALELKLFTVIGKATWLLP
                     DLARELKVSERGLSILCRNLAMAGLLYKHGNRYRNSPLAATALNADDPNYRGSYLDLI
                     TNHWSDWLRLLESVKSGEPLDKDEPEEPDYRRRFTWAMHHRTLETAPIIAAQLPLKGV
                     NTLLDLGGGPGTYAMAFLAKHPRLRATVCDRPAALEVAKDIAATHKAGARLSYLPLDL
                     LTDDIPGPFDVIWYSNVLHIYSPAQNQDVFRRAKEALADGGRLVIQDAFLHDREGLFP
                     EEASLFAVSMLLFTEGGNTYSVAETSAWLKDAGFDSVRPVRMKKGKEDWEGGILEAAV
                     LRGRSKRHGHRTRSAKNSHTR"
     gene            complement(23169..23606)
                     /locus_tag="VFH05_14670"
     CDS             complement(23169..23606)
                     /locus_tag="VFH05_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679516.1"
                     /translation="MNDRKSPRWTEARSTLSKAFPQLYELEPDGPLALDLGQDGWLLE
                     ITPEGKLLCQYGVAMDDVMSLMSEGTPEDLGTDEVAKQAKYFIQPAVSKYRPLLLQSG
                     FSEQTEMNEEYVAVSFERQVDLENLSKVQDLMRWCCRQIGGAA"
     gene            complement(23603..24430)
                     /locus_tag="VFH05_14675"
     CDS             complement(23603..24430)
                     /locus_tag="VFH05_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6679517.1"
                     /translation="MTLKNGLSEELNRQGNEHFGRGHYTEAYTCYAKALECDRLTGDQ
                     RALAATLGNLGNICAVSGRRDAAQNYYQEVLELQKIFGDEKGIGTTLANLGNLRADAG
                     EWDRARAYYLEALDLMTKTQDETSKAVLFSDLGLIAREKGEFNEAIAHYEKSLVLMRR
                     LGNDGGVADAWRMLARTFLLQQRFDDAIACCQTSQSIAERSQDELRTGGARYVLADCY
                     EAMGRFREAAQLLERVVEMDRKYHLPKLGENTKRLEGLRQRLAAENATTAARKESHG"
     gene            complement(24498..25103)
                     /locus_tag="VFH05_14680"
     CDS             complement(24498..25103)
                     /locus_tag="VFH05_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679518.1"
                     /translation="MKPIFPGIWQWSWFSEEKQLNFNGLFLTVGEHRILVDPPPLTAE
                     ATTVIRRQGQLDYIIVTNRDHAREAQACRRDFGCQLFLPEADAPQMDLKPTRTFKDGE
                     LLPGGIWVIHLLDQKSAGESALFIQQGKGVLIVGDALIGKPPGALTMLPEEKYHDVVK
                     AKEGLRRLLKYNFDAMLVGDGTSIMTGAKAAVEKVLAEHGS"
     gene            complement(25146..25385)
                     /locus_tag="VFH05_14685"
     CDS             complement(25146..25385)
                     /locus_tag="VFH05_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679519.1"
                     /translation="MDEMEEGKQKFLALVRDIDQQVQVVIPVTPSNSMFLISLTKGAN
                     RKFITIPEDDIIDLPHESGILTKVTKTIKDAVAAL"
     gene            complement(25544..26080)
                     /locus_tag="VFH05_14690"
     CDS             complement(25544..26080)
                     /locus_tag="VFH05_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6679520.1"
                     /translation="MKRRWVILTVLLWPVLTAHLLLAAGFFKVGERAPSFALNAITGE
                     PVSLDANKGKVVVLGLFHICDPCMMQGTNLQKVYEATQGKNVAVIGVNSSGNSKKDVG
                     EFLSAFPVKVTYPYLLDPGKVTDKMYGGGKFIPNVYVIDQQGVIRWQRVGNMELAGSD
                     VIIAEVEKLLAAEGGNKM"
     gene            complement(26230..27288)
                     /locus_tag="VFH05_14695"
     CDS             complement(26230..27288)
                     /locus_tag="VFH05_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679521.1"
                     /translation="MSQLVMGLRAEHTITGDSREHRFSFHGIPFRFCSSSASLSSSIA
                     AWVRTVDSGDVKGDVSLTAHFEHVSSREAVPVPLSPGARRVFSGTMPALGNSLRALWQ
                     CDVYRDGERLVVDVRDHGVLLIDRNRSAAAGHFYCPETMAPDRLETFFHYTMIELLKG
                     KNTFPLPAAALEWKGKGVVLFGPNGCGKTTAMLSLLRSGYRYLGDDISLLRDHGPMQL
                     LPGASPTIDVTDHTIEMVPELRTAGLRLLRQGAYRKSFHPEDVYPGSRGRSCEPAMIL
                     FPQVTNISHSCLEPLAKSLALEALVRREAHKHDADTAAQEFQVLSKLVQRAACYRLHF
                     GRDVLDLPRLLTPLLERH"
     gene            complement(27310..27591)
                     /locus_tag="VFH05_14700"
     CDS             complement(27310..27591)
                     /locus_tag="VFH05_14700"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET6679522.1"
                     /translation="MHLAPAKNPNVRGVVLEGETVLLDLVTGQSFRLNAVGTAVWEHC
                     TGTATLEQIHHTISARLEWHVGDLREQMIACVVQWSHDRLLHQPDGAGR"
     gene            complement(27851..29281)
                     /gene="purF"
                     /locus_tag="VFH05_14705"
     CDS             complement(27851..29281)
                     /gene="purF"
                     /locus_tag="VFH05_14705"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET6679523.1"
                     /translation="MRKELPIAGSDKFHDECAVFGIYGHKEAANLTYLGLYALQHRGQ
                     EASGIVSADGDHFHVHKGMGLVADIYNTQTLKNLTGSMAIGHNRYSTAGGQDLKNVQP
                     LTVNFAFGNLALAHNGNLINAQVLRNELEAYGAIFQSTSDSEVIIHLIAHSRADTLLA
                     RVIDALSQVRGAFSVVLLTDEGIIAARDPYGFRPLSLGKLRDTYLVASETCAFDLLDA
                     EIVREIEPGELVVLNENGVTSYKPFSAVQPAMCIFEYVYFARPDSKIFGANAVYATRK
                     ALGRQLAQESWVPADLVIPVPDSGVPAALGYSEGAGIPFETGLIRNHYVGRTFIEPEQ
                     SIRHFGVKVKLNAVPEVLHGKRVVVVDDSLVRGTTSRKIVKMIRQAGAKEVHMRISSP
                     PIISPCFYGIDTPTKKELIASSHSAEEIRKYITADSLAYLSLDGMLKSAPGAPHHYCN
                     ACFTEQYPISFTRAEEFQLGLFERSS"
     gene            complement(29290..31539)
                     /gene="purL"
                     /locus_tag="VFH05_14710"
     CDS             complement(29290..31539)
                     /gene="purL"
                     /locus_tag="VFH05_14710"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET6679524.1"
                     /translation="MNTNTADVKDLLAQHNLTEDEFKKIVDILGREPNLTELGMFSVM
                     WSEHCSYKSSRVHLKKLPTTGPRVVQGPGENAGAVDIGDGLCVVFKMESHNHPSFIEP
                     YQGAATGVGGILRDVFTMGARPIALLDSLRFGSLDSAKNRHLMRGVVAGIAGYGNCMG
                     VPTVGGEVRFDDIYALNPLVNAFCLGIAHKDKIFRGTAAGVGNPVIYFGSKTGRDGIH
                     GATMASDAFDDDSDQKRPTVQVGDPFQEKLLLEACLELMANDLLVGIQDMGAAGLTSS
                     SCEMASRAGNGMELDLTHVPRREPGMTPYELMLSESQERMLMVAQAGKEEECIRICRK
                     WDLDVAVVGKVTSDGLLRVIDQGKVVAEIPAKSLADDGPRYERPYSPPPYQEMLTNLN
                     YDSMPDVKDANAALLSVLASPTIASKRWVYEQYDHMVRTNTLVRPGSDAAVVRIKGTN
                     KAVAMTVDCNGRYCLLHPYEGTRLAVAEAARNLVCSGAEPIGLTDCLNFGNPERPDIM
                     WQFVLAIEGLKDACEHFQIPIVSGNVSFYNETNGLSIYPTPMLGMVGLIDKAEQAMTQ
                     WFRHDEDAIILLGKTRDDLGGSEYLKVMQHREQGSPPLLNLETEKALQDFVLTVIREG
                     LVESAHDCSDGGLAVALAECCVSGPDVRRGAVVKLELGSLRRDALLFGESSSRVILSM
                     RPDTVEALLSRAADAGIPAGRIGTVGGDRFIIDVAKGRMNEGCRIDLPIDRVWDRWAH
                     ALEEQLRHE"
     gene            complement(31594..32310)
                     /gene="purQ"
                     /locus_tag="VFH05_14715"
     CDS             complement(31594..32310)
                     /gene="purQ"
                     /locus_tag="VFH05_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET6679525.1"
                     /translation="MNIGVIVFPGSNCDHDCEHVFKDVLAQNVTMVWHKETSLVGLDG
                     VILPGGFSYGDYLRTGAIARFSPVMSAIKEFAKSGGLVMGVCNGFQILLEAGLLPGAM
                     LRNRSLHFVCKDVHVRVENAATPFTGACKPGQVLKIPIAHAEGNYYTDPVMLAGLQAN
                     AQIVFRYCTPDGRVTLEANPNGSLDNIAGIRSAEGNVLGMMPHPERSAEELMGNEDGR
                     LIFTSMLESLKKRPLKMVGA"
     gene            complement(32313..32552)
                     /gene="purS"
                     /locus_tag="VFH05_14720"
     CDS             complement(32313..32552)
                     /gene="purS"
                     /locus_tag="VFH05_14720"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET6679526.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAANVRVGKYMEL
                     DVSETDKAKAEAQVKSMCEKLLANTIIEEYRYELV"
     gene            complement(32549..32767)
                     /locus_tag="VFH05_14725"
     CDS             complement(32549..32767)
                     /locus_tag="VFH05_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679527.1"
                     /translation="MLKAFLNYGLVAAVLVWAGVVAMMAYRLDDSPWRWAFAALAVGG
                     IATVAVLFKIRRYVDGLHKQSGEQETKT"
BASE COUNT         6908 a         9943 c         8984 g         7260 t
ORIGIN      
        1 ctgcccgcga tcaatttttg cagtcttact tccatatccc tcttcgacgg acgtcccggc
       61 tggcccctac tgcacacgca tttttattcg ctcaatgcct gatcaaccct actcccattc
      121 gatggtgctc ggtggttttg agctaatgtc gtagacgaca cggttcacgc ctttgacttc
      181 gttgatgatt cgattggaca tccggccgag gacctcgttg ggaatcttcg cccagtcggc
      241 ggtcattcca tccaagctcg tcacggcgcg aatcgccacg acattctcat acgtccgctg
      301 atcgcccatc acaccgacgg tccggatggg cagcagcacc gcaaacgcct gccaaatgtc
      361 gcggtagagg ccggcgcttc ggatttcttg gtccacgatc gtctccgccc cacgcaaaat
      421 ggccagccgc tccttcgtca ccgagccaag cactcgaatc gccaacccgg gacctggaaa
      481 cggctggcgc caaatgatct catccggcag gcccagctct tttcccagca cccgcacttc
      541 gtctttgaac aattcacgca gcggttcgat cagtttgagc ctcatccgcg ccggcagtcc
      601 gccaacattg tgatgggtct tgatcgtcgc cgaaggccct ttgaagctga tgctctcaat
      661 gacatcggga tacaatgttc cctggaccaa atacctgacg tccttcagct ttcgggcctc
      721 ggtctcaaaa ttcctgatga acagacgccc gatggccttg cgttttcgct caggatcggt
      781 cacgttcttt aagcccgcca gaaacgctcc tgtccgatcg agtatccgga gattcagatg
      841 cagctgtgag gcgaaggtct tctgcacctg atcgcgctcg cccgcccgga ggacgccatt
      901 gtcgacaaag atgcaggtga gctgatcgcc gatcgcccgg tgggtcaggg ctgcggccac
      961 cgatgaatcc acaccgccgc tcaacgcaca aatgacgcgc tcaccgccca cttgttctcg
     1021 gatctgcttg accgccgttt ccacatagga ctgcatggtc caggtcggct tgctgccgca
     1081 tatctgatag acaaagttcc ggagcaactg ggtgccttcc ggggtatggg cgacttcagg
     1141 atggaactgc aggcagtata tccgccgttt gccatcgact cgcttcatgg cggcgaccgg
     1201 agaattttcc gtatgggcga tggaccggaa tcccggcggc atccgttcga tgcgatcgcc
     1261 atgggacatc cagacgaccg tggagcggtc cttgccgatg cccgcgaaca aatcactcgc
     1321 atcgtctatt ctcagctcag cccgaccgta ttcgcggtgc tttgatttcg cgacttctcc
     1381 gccggaaaga tgcgtcacca gctgcatgcc gtagcagatg ccgagaatcg gaatgccctg
     1441 atcgaacagt tccttcggaa ccgccggagc cttcttttca tatacgctgg acggaccgcc
     1501 ggaaagcacg atgccttgcg gtctataagc taaaatcgtg gcgagtggaa cggtgcaggg
     1561 aaggatctgc gaatagacct gcgcttcccg aatccgtcga gcgatcaact gcgtgtattg
     1621 agagccgaaa tcgaggacca ggattctgtc gtgccagagt tccatcgttg acaaagcgta
     1681 tcgtgtttat ctgagtggct gttaacagca gaaaagaaat cggcaggcag agaagacagc
     1741 gttcagcgtg tcctatcggc tagacgccat cgctcccgtt tattcccagt ccatgcgata
     1801 gttcggcgct tctttggtga tgatgacgtc gtgcacatgg ctttcacgaa gccctgcaac
     1861 cgtctgcctg ataaacgacg cgttcttctg gagttcggga atcgttttgc atccgcaata
     1921 gcccatcccg gacttcaccc cgcccaccaa ttgatagatg acagcagaca gcggaccctt
     1981 gtacggcaca cgcccttcaa tgccttcggg taccagtttc tgggccgggc gcccgctctg
     2041 gccgtagcga tcgccgccgc ctcgttccat cgcgccgatc gagcccatgc cgcgatagac
     2101 cttgtaggtt ctcgcctgat agagcaccgt ctctccgggc gactcctcgg tgccggcgag
     2161 caacccgccg agcatgacca ccgaagctcc ggccgcaagg gctttactga tgtcgccgga
     2221 aaatttgatg ccgccatccg ccacgatcgg caccccgctg ccctcaagca ccttggcgca
     2281 atcggcaatc gccgtcagtt gaggcattcc ggagcccgag acaatgcgtg tcgtgcaaat
     2341 cgagcccggt ccgacgccga ccttcacagc atcgaccccg gccctcagga gatctttcgc
     2401 tgcctcagcg gtggcgatgt taccggcgat cagttcgagt gacgggtgct gtttcttgat
     2461 catcctaact gtttcaagta ccgcctgcgc atgaccatga gccgtatcca ctaccagaag
     2521 gtccacgccg gcttttttca gctgggtgac ccgatcttca atgtccggtc cgacccccac
     2581 cgctgcgccg acacgtagcc gtccatgagc atctttgcag gcgttaggat atttgatccg
     2641 cttttcgatg tctttaatgg taataagtcc cttgaggtca ccgtgcttgt tgacgaccgg
     2701 taacttctcg atccggtgtt cgtgaagaat ctcccgggcc ttttcaaggc tggtcccttc
     2761 aggcgccgtg accagtttgt cgcgtttcat gacctggctt actttaaggt ccaatcgcgt
     2821 ctcgaaccgg agatcgcgat tcgtgagaat gccgaccagt ttcttgcttt tggtcaccgg
     2881 gattcccgaa atccggtatc tcgccatgag ttcgtgagcg tcacggatcg tttggtccgg
     2941 tgagatggtg accgggtcca ggatcatgcc gctctcggat ttcttgaccc ggtcgacttc
     3001 cgccgcttgg tcggccggcg tgagcacgcg atgaatgatt cccacgccgc cttccctggc
     3061 catcgcaatg gccaggcgcg actcggtgac cgtgtccatc gcggaactaa tgatgggaat
     3121 gttgatttga atgttgcgcg tgagctgggt acgaacgtcg acttcgctgg ggagaaccgc
     3181 ggactttgag ggcacgagaa ccacgtcgtc ataggtgagt ccgaggcgtg gttctttctc
     3241 gagcatggtc ctcccgttct ccggccgtca ggccttctgt ggttgttggg ccttttcgat
     3301 ttcggccgcc gcttccgctt cttcctggag ccgctcgctc ccttcgtcgg ccggcatgaa
     3361 ctgctgcacc cgcgtgttgc cgctgtcgac ggcgaagacg ctgcctttcg catcgaccgc
     3421 aatcccgtag gggaaattga attggccgtc cccgttcccg tagccgcccc actgcgtgat
     3481 gaaattccct tccttatcga atttctcgat gcgatggttg ccggtgtccg atacatacac
     3541 gtcgcctgca ccgtcgacgg caatccccca aggtgaccgc aattgaccgg cctcctgcga
     3601 caaaggactg ctggcatgcc ccgcctcgac gctaccaccc catttcgtca ataactgagg
     3661 taacacgttt gtgctggtat cgaacttctg aacacggtgg ttgcccatat cgaccacgta
     3721 gacggcccca tcggattgat cgacggcaat gccgcggggg aaatagaatt gcccgtcacc
     3781 attgccgaag ctgccccagg acatgatgaa ctctcccgtc atgtcgaact tctggacacg
     3841 gaagttcgcg ctgtccacca cgtagacgta cccgcgtacg cggtctacgg cgatgcccca
     3901 tggcgaattg aactgtcctt cgccgttgcc gcgcgagccg aacttcatga ggtagccgcc
     3961 caattttcca tcgaatttct gaacccggtg gttgttcgta tcgacgaccc acacgtcccc
     4021 tttgccgtcg cacgcaatgc ccgtcggatt gtggaaattc gagttggctg agccgaagtt
     4081 cccccacaag atgataaagt tgccggcgtt atcgaacttt tgaatccgat tgttgccgtt
     4141 gtcaaccacg aacaaggaac cctgctgatc gatgcacagg ccgtagatcg gcgccatgaa
     4201 ctcgccgccg tgtaggagcg aggcaccgcg accggaccgt ccccacctgg acacgcagag
     4261 atagccggat gtattgacca ggatcgtggc actcgccggg gtggacacgt tgccgccgac
     4321 gtccttgaac cacacaaaga tcgtcttctg accgtcgcca ggagagagga tgaaaggaat
     4381 ggtcgcgccg aacttcaatg atggcgtgac ctccacccaa ccgggcgtcc ctgccatcgg
     4441 ggtcataggc gcttcggaca catagtaggc cccgacgccg gtatcgatgt cgttggctga
     4501 aatggtgacc accacttccg gcgaattcgt catgaaggcg ccgtgattga tgacggcata
     4561 ggggttttgc ggagccgtaa tgtcgatcag gaccggcgtg gcggtgacga cgtgtgacaa
     4621 ctcgctttcg acctcgccat cgaacatcgc cgttatcgca taataatacg gtgtatcgtt
     4681 cgtcagtccg gtatgcgtga agggattggt cacgccttcg atcttggttc cactttgggt
     4741 cgtcaagagg ggcgaggtat tgaaatagag gttgtaggag acggcgccgg gcacttcaat
     4801 ccagctgaga aatgtctccg tatcgccggc cttcaccgca acgttgctcg gaggcggcat
     4861 cgagttggtt ccctgcgact tgggcttctc ttccttgccc ctgttgatct cctcgtccgt
     4921 gggcacgtac ttgaggatgc ggttattgcc gctgtccacc acgtagacgc agccctcctt
     4981 gtcgactgca atcccgtaag gaaagttcaa ttgtccttcg gatttgccgc gattgcccca
     5041 cgcgcacagg aacgtgccgt ttccatcgaa cttctgaatg cgatggttgc cggtatccac
     5101 gacatagacg ttgcccaatg catcgcaggc gatgccccat ggcgctttga actgcccggg
     5161 cccggcccct tccctgcccc acttggtcag gaagctgccg cgggcgtcga acttttgaac
     5221 ccggttgttg ccttcatcgg cgacatagat gttgccgacg aaatcgaccg ccacaccacg
     5281 cgggaaaaag aaggccccgt caaaactccc gtcgcggccc catttcaaga gaggcgtccc
     5341 atccgctttg aacttttgga tgcgtgcgtt gctggtgtcc gagacataca gattgccgtc
     5401 ttgatccgtc gccagccccc atggcacatc gaatttgccc atatcggcgc cacgccaggc
     5461 aaagccaaat ttgccccagg cctgcatgac gttgccgtcg agatcaaact tttgcacccg
     5521 gttattgccg ctgtcggcaa tgtacaggct atcgttcgcg ccgaccgcga ggccgcgcgg
     5581 ataataaaag ctgccttcct gagacgaggg ctcgccgccc cagcgcgcaa tgaagtgtcc
     5641 ggccttgtcg aacttttgga tggagtgatt gtcggtatca gcgacgtaca gattaccgtc
     5701 cttatccagc gcaatgcccg tcggcgagct gaattctccg tcatcgacgc cttcctgacc
     5761 gatgcacgcg gcaagcagat agggagaggg aatcgccatc acttcttgcg attccagact
     5821 ctcacctttg gcggtcacga cggtcacgac gtagtgatag caggtgccgt tggccagatc
     5881 atcgtggagg aagggagact gcgccccttc gagacaggtg gccttttcct ttttcactcc
     5941 gataacggtt ttgaaatcct ctggaccggc gataggacgc gtcagctcag aaaatttgat
     6001 gtgaacgccc ttggtcgtct ggaaatagag attgtagtag agggcatcag gaactgggtc
     6061 ccaggtaata gtaatccggc catttccgca cttggctgct acattcgcag gtgccggagg
     6121 cagttcctcc tcttctatga tcgaatcgcc agcgccccat tctccgtgac tgccttccac
     6181 cgtcagcggc gcacgatcga atcgaaacat ctcatccagc agccaggctt tgatcataag
     6241 tggtgcctct tgttcctcga cagggttaca accaagaaac tagcatcttt caatgagtta
     6301 cacaggattg gggagtctaa caaagcgaaa agctgaaatg caagggaggc aggaggcggc
     6361 aagggctgtt ggtctcaaaa tagcgtgcgc gcgcacgtta ggactgatcc aggctgctcc
     6421 gtatgggagg aacaagctag catggaagga tcatttgtaa ggcggtgctc ccagggaaaa
     6481 gcgtgcgtcg cgggtaaccg attgcgaagc atcggggtgg gctgggtaaa aaaagattgc
     6541 gcagcgttgg gcgggtgaga agaatacgcg cagtaggaga ccaaccagcc ctgcctccga
     6601 ggaacggcga actcggacgg actatttaag tcgcagcgtc attgagggcg gaggcgtacc
     6661 tcaaaagcgg gactcggagg tggccgctaa gagataggaa tgagcacgga tgctctttag
     6721 ctctgttcca ccgcttcagg gatcaacgac tgcgattccc acgccagctc tggatccgtc
     6781 gagagagcgg gctccgaggc ggcagcggca acgtcctcct ggggcatcgg ttcctgttcc
     6841 atcggcagca gcgcctgctc cgcttcgccc aattctttaa attcgcgcag cgggggcaac
     6901 tgggtgatgt cctgcaatcc gaaatgttcg aggaaaaatt tggtcgtccc atacataatc
     6961 gggcggcccg gcacctcttt acgaccgacg atgcggacca gtttccgttc gagcagtgtt
     7021 ctcagcaccc ctgacgtttc gacaccgcga atgtcttcaa tctctgcccg gaccagcggt
     7081 tgcttgtagg caatgatggc cagcgactca agcgcagacc gggacaattt ctgagcagct
     7141 ttggccttgt ccaaccgctt caaccatggc gcatagtcag atttcgtcac gagtcgatac
     7201 ccgcccgcaa gtttcaccag ctgaattcct cgaccttctc gagcgagctc ctgctcaagc
     7261 gccttaagcg cctgctccac ttcggacttg gacactgttc caatcacggt ggccaaacgc
     7321 gagaccggca ccggctcggt ggagacgaac aacagcgcct cgagcacacc gatcaattcc
     7381 gttcctttca ggtcctcctc ttgcggttca agctgagccc cgcccgattc cgacgctgcc
     7441 gagtccagtg ccccatttcc attgaccgcc tcgtccgcac tcactatagc tgcatcctgc
     7501 agcgccgctg aatccatgcc tacctccatt ccgagtcatc caattccgcc ggatccggta
     7561 ccaacgagaa ggagcgtgac accaaaatcg gtccgaagct ttctgcctga tagacgcgca
     7621 ccgttcgcaa ccgaatgagt tcgagcaaag ccaaaaaggt cacgataatc agcagccggt
     7681 gacaggaggc ctcaaacaac gcctcgaagc cgattgactc ttgtccttcg agggcctcca
     7741 agatggcgtt catccggtcg cggacggtta aattatcggg aaggatttcc agcagcttct
     7801 tcccaggatt gcgattgaga atcccctgaa gcgcgtccac gagatcgaac aacccaatgt
     7861 tctcgagcat cgcctcatcg gtgtccgggg cttcttcgtg aggtgctgcg ggtcggctgt
     7921 aaatctcccg ccacatcttt tcctgcgtat ccagctgccg tgctgcttct ttaaaggtct
     7981 tgtactcaag gagtctgcgg acgagttcct cgcggggatc cggtccgtca tcttcgtctt
     8041 cggcggtctc ttccacagga agcagcatct tcgatttgat ctgaagcaat gtcgcagcca
     8101 tcaccagaaa ttcaccggcg acggcaagat tcaactcctt catcgcctcg atgtagctca
     8161 gatattgctg agcgatcaat gcgatcggga tatcgtagat gttgatctcg ttcttcttga
     8221 tgaggtgcag caacaggtcg agagggccct caaagttctc aatgcgaact tggtaggcga
     8281 actcggtttg ttcggccccg tcgagttggt tatccacagg gtggtttata ccagctttgg
     8341 ggagagtgag caagagccga tctattagtt gtggtttatc tggaccgaac cagataggcg
     8401 atcagcacgg cactgaagag caacagcgag agggtaatga cgtattgaac ctgacgattc
     8461 tcgattccca acgaatcagg atgggtcgca gaccgcctgg ctcgggtgaa ttgcggcccc
     8521 ttctcgaaaa acgttttgga gaaatcttcc cgtgcattga attgcgcatc atcgaaatcg
     8581 gccgtcgcgc ggaatgtcgc gcgccggaaa aaggcatcgc catcgaatcg cgtgaaggtg
     8641 aagaaggcgt ccccgtcgaa cagcgtctcg gaaaaatcag ccagctgctt gaagtgactc
     8701 ccagagaatc ctgtcccgcg tttgaaatag gtccgcgaaa gattggcatc ctgatcgaaa
     8761 cgaacttcga ggaactcggc cattccactg aagccggatt gttgaagggt caccggcccg
     8821 tggaactgcg accggtgaaa tctcgtgtgg gatccgaagg ccgttttttc tccagtcacc
     8881 ccacgcagaa aatgacattg cacgaaatac ccttcccgta agaacactgc acccgaccat
     8941 gcgaccggtt gcgtgaagac ggatctcgac agatcgacca tacgctcgaa cgtggtcccg
     9001 acgaacgtga cgggaccgga gaacacgagt gtgcccaccg ccgtccgatg ggtgacggtt
     9061 cctcggacca ccgaatcgac gatggcaacc ggcgccccga tgactcgaat ctcattgtcg
     9121 tcgcgatcga tgatgtcatc aatgacgggc ggactctcag tcaccggcag ggcatcaagt
     9181 gcaacgtcac cttgaatcac gactccggca agatcgatgg tttggccgcg tttcagcgcg
     9241 gctaggactg ccgaggcatc gaccgcacga gcctcccgct cgacgggcgt gcagccagca
     9301 ctgagatggg ttttgaagag ccgttgggag ccagctaccg cctgctctac gacgcactct
     9361 gctgcggcat agtccgcaaa gcagtacagc agcacaagca ggatggcaac agctctacca
     9421 ggtcgagtca tgagggccct tatacggaat gcctccggct cctggcaagg ccggctatga
     9481 tgacttcctt tacgagactg cacggtctgc tacactcttt ccatgctgat gcgcacactc
     9541 atcgccggct cgctcttggt tggttcagtt gtgctgacta ccagtgccag ttacgccctc
     9601 gattacgttg aactacccgt cctcgcagcc gttcgttcta acaatctggg cgtctttgaa
     9661 atcatgctaa tggggtggga caaaaaggct gatccgaatg cggttcaact tcaggtgtac
     9721 attgcaggcg ttcggtacgg caaacatcac ctgggatcca tggtgcaggc gttcgactac
     9781 gcggtcgagc gcactcctgc agtgctccat acgggaaccg tctccgtcca gggcgtgacc
     9841 tacctgccaa caacgagtga cggtccgagc gccggcgcag caatggccgt agggtttatt
     9901 gccgtgttca agggggaccg catccagcgc gggaccgcgt taaccggaac gcttgagcca
     9961 ggtgggcaca ttggccgagt cggcagcctt cccgacaaga tccgtgcggc caagcgggag
    10021 ggctaccata cggtgctcgt gccgcgggga caacttcaca cgagcgaatg gaatctcatc
    10081 gagcttgggt ttcaactcaa catctccgtc aaggaagtgg acacggtcga tgaggcctat
    10141 cagttcatga cgggcaatag attgtagcgg aatggagaat cgtcagcctg agtattcccc
    10201 tgctttccta cgtcttgtga cctgatcccg tgccgcttcg aatcccttcc acgatcgctg
    10261 tgaattcttc cgccgtgagc ctatgcatgc ccaaggcgag tcgtcgagcc aattcggcct
    10321 gatctggaat cttcagcaca tgctggagta acgcctgact cgcctgcgga gtccatgccc
    10381 atcgcttaat cccctggatt cggataaagc cgaatcgtgc ctcggtccgc agatcatctt
    10441 taagaaactc atccagtttg gcaaaatcca acggcggagg cacgatcgta aactccgcac
    10501 ataagccagc cttcagcaca tacaccaaac cacatgagtc aggcgtgaga tacgtttgca
    10561 ggttggttcg aatcagggcg gtacacagcc cccatagacc atgagagagc tgcagctccg
    10621 cactctcctc cgacgtccgt tcgcgaagcg cggccgcgat gaacagaaaa tggctcacga
    10681 gatggacctt tccatatgag agtccgcgag cgcggcgacg gcagcaacag ggtgaagaga
    10741 acgctgtcag gccggatgtg aggtgaggct gtatgactca gtgacggtag tcgtctgaat
    10801 cgtcaaggag gtcatcggat ttttcgaggt aatcgacgtc ttcttcatcg tcgtcgagcg
    10861 agagatcctc ttccatctct tcgtcaattt cactttcact ctcactctcg ccggtgagat
    10921 cgatctcttc ctcacccatc tcctcttcat ccagatccgg ctcttttggc gccatcgccg
    10981 gagcgccctt cgccgtcgtc ttcctgagtt cctcgtcagg accagccttt ttagcgaccg
    11041 caggtttgga tggaacaggc actccaacgg ccttctttgc gggagtcgcc ttcttggcaa
    11101 cggactgctt cttccccggt tttcttaccg gcttcttcgc ggactttttg gtcttcgtct
    11161 tggcggtgtg tttcttcgcc ggacgctttt tcatcttcgc catcgatctc tctcctctct
    11221 catcttcaaa cacttgatgt catgcggcct ccatctagca tgaacgtacg tagtcttgca
    11281 accagggtct tccgccgcgt cgttcggcgt catgaccgct ctggtcggcc tctgaagggc
    11341 gcttcgagtc ctgctctcta tgggcctctc acggcgtgat ccacacggtc acgaccgccg
    11401 gcacactgtc cgctcccttc ataacgtccg gaccggccat gtcggtcacg acgagagaaa
    11461 attgataccg tttccgttcc gagaccttcg gcgcgacaaa tgtcgccttg gcctcgttca
    11521 cattactcaa catcacgcga tgacccagaa tctgtttcca ttcgaaatga agaggatcgc
    11581 cgtccggatc ccggcttcgt agaccatcga gcagtaccag ctcgccggat ctcgcgtgga
    11641 gttccttaac cacttcagcg ataggcgagc gattggattc ttcatcgctt tgaacgatca
    11701 cctcgactgc gccggtcttt ccaccatttt ccacagtcac gatcgccttg ccacggccaa
    11761 tgacatgcaa gactcccgtc gcatcaacgg acacgactcg ttcatccgac gaatgaaacc
    11821 ggctgcctgt gcttgatccg gagagcggac gccacacgcc gtcgctgaac tgcccaatgg
    11881 ctggaacgac catccgccta ccgacttgct gcattttcca aggtttctcg acggcaaatt
    11941 cgatcgtttg cagttcagca gtcgcctcaa ccgtgaggag cacttcgtcg aactcttcgt
    12001 ggctctccaa gcgccctctt acgacctctc ccacagccag tagtcgcatc gtgcccagcg
    12061 catcagatgg cacaaggacc gttcccgcaa aaggagattc acctctgatc ggcttgaacg
    12121 gagcgggatg agcttgattg gacgcgagag gttcctcatc cgggcggtac caataatagt
    12181 ggatcgttcg cagattgaac tccttcccca cggatacctc aacggtgaca agccggccag
    12241 cctgcaatgt cgcgccctgc gaaggcgatg taagggtaag cgcccaaact gagtgtccgt
    12301 ctaccaggcc cacaaggaat aagacaatcg cgatcgatcg gcacatctgg ggttccctcg
    12361 gaatgaggcg gaagaatcgt acaatccagc cgctgcgtca aggtggcagt gcaggaatat
    12421 cgccctcttg acgactactt ctctccttga cctgcgtggt agaatggtta cgaggaacgc
    12481 agagcatgac aatcgccttc ttcagacgca cccttgtcgc gatgctgacc atgctgtcct
    12541 tgactgcctg gcccacgacc gccacgcctc cctatacggc catcatcatc gatagcggtt
    12601 caccctattt tgttcccaag tcagccacag tctcgaccgg tgcgcccatt cgatgggaga
    12661 atcccacgcc gaccgagcat accgtcacgc acgtcggctg tttggaggac ggagccgggt
    12721 gtgcctttga ttcagggctt gtgctaccaa acgattccta tacgcatccg ggcctcgccc
    12781 ccgggaagta tcaatatatc tgccgcatcc atccaatcat gcacggaacc atcatcgtga
    12841 cggactccgc tccagcgcct tctcaactgt aaccactgcg cctaggttct tgcacgaaaa
    12901 cggtctgctc gtgtacgctt ccgcgcgtat ctagggacca ccatgaagcc ggctgtcgct
    12961 gtacacgaac cccgcaatct gacaggagaa actctcaacc tcctggcctg gcacattccc
    13021 gatttgattg cttatcaaca ccatgccgat gaatggtggt ttgcccctct cgatgacaat
    13081 ctccccattg tgcgggtgaa ccgcattggt ctcgagatgc tcaaggccat gaacggccat
    13141 acgactgtcc gcgccctgat cgaccggtac gggtcaagaa tctgcgggcc agacgggcag
    13201 ccgggacagt ggcatctcga acggtgggcc ttaccgaact tttccttgtg ctattacggg
    13261 actgagcctc ccggcggaca ccggcatcag gccaaatggg atgtcctcct gcagcagata
    13321 cgcgaaggat ggtccggaca agaggggttc gagggtgaag aacgtttaca agatttccat
    13381 gtgcacgacc tgaaagacag ccctgaggat gatgagcatt tcgacctgat tgaaacgacg
    13441 gtgtcccacc tgtttcgcga gcccaacgaa gctttgaacg gcctgaccta tggcaggctg
    13501 gtgatgcaaa aactccgacg cctcgggtgg tttaatccga aaccgcaggt catcgtcgaa
    13561 gtgggcgggg gcctcggcta tgtcgcccgt gaactcggca aagagttgtt gccgtttgaa
    13621 aagcagggca tcacgtactg ttcggttgac attacggccc catttttaca gctccaactc
    13681 tctcgcgcga gagcgggcgg gtggcagtgc gccggcgccc aggcgaacgg agagtggctg
    13741 cctttcaagg atcattccgt cgatctggtc atcgacaacg aaaacatggc cgacatgacg
    13801 cctgtcaaac tgacgaagca ggaattactg acaggcaccg gcgccacccc gccacatcag
    13861 gaggcgctgg attggatcag gcgcgttcgt ctgcccatcg gcaaggacct gccggaggac
    13921 gtcatcttca acctggggcc tttccgattc gtcagcgagt tgtggcgcgt tctgaaaccg
    13981 ggcggacggg ctttcctgac ggagtttggc atcgaagagg gctggccggc cccggtcaaa
    14041 ctgcccggac ataccgaata tgaggtgcaa tacagccacc tgcgccaggc tgtccgctgg
    14101 ctcggatttc aagaacagta tctatcgctc ccgcaattcc tggcgatgaa accggatacg
    14161 aaggtcttgt gcaccggggc ggcgtacacg atccaacggt tttgccaggc gatgaacaag
    14221 cccttcgtcg tgcgcgccta tacggaacag gaactgagcc atgcgcttgg cgatctgctt
    14281 cccaagtttc aaggcacaca ttaccatgac gtggcagacc cggcctggtt cggtctcgtc
    14341 gatttcaaag tgctcttatt agaaaaacca ggcggggcgc caaaaccaac ctttacggaa
    14401 accaaaggtt atcgctggta cagtcagcgg tgatgcggac ctgctgccct tcatgctcgg
    14461 gtctgcggcc ctgtgtgaag actatgcctg ccttggtcgc cacatgcccg ccggatcagt
    14521 cttgtcggtc tttgtgggag cagcacagca gaccaatcgt tttactgact ctctctaatg
    14581 tcgcgtcgcc aattcgatca ggactccttc acgtaatcca cgatcactga cgagacattc
    14641 acgttcgccc aggatgtcca tcaccgtccg gaggatgatc gctccggctg caatcacgtc
    14701 ttcgcggttt ctttccaacc ccggcatgcc gattctagct gctttgcttc ggctcaatag
    14761 gtgcttttcc aggtcggtga tcgtattcaa cgccagccga taattgtgaa ttcttgctgc
    14821 atcataagca ggaagctgca gtgccattgc ggccaaggag gtgatcgtcc cggcggtgcc
    14881 aacgagtgtg aggccggtcc tttgcggcat tgcggccaac acgaccgttg tttctctttg
    14941 tatccaggca cgcgcctctg ctatctcctg tctggtcggc ggatcatgat gcagcagacg
    15001 ttcggacagc cgtacgacgc cgatatcgat cgatcgcacg acgggcgact gcccctgcag
    15061 tgtcaggata aattctgtgc ttccgccgcc gatatccagc gccaggacgt cttttatgtc
    15121 tccgggtagg cccgaatgaa tcccgagcat cgtccggcgc gcttcttcgt cgccgctgag
    15181 gatttcgact tcgaagcctg cttctgatct gactcgattc agaaactcct gcttgttagc
    15241 ggcatctcgc acagcactcg tcgccaccgc aacgtgtgca tgaacatggt agctctcgat
    15301 agtggcccgc cagtctttca ggcagtgaat gacccgctct acggcctcgt gtttcaatcg
    15361 tttcgattga tcgacgcctt ctcccaatcg caggatccgg cgctcggagc acagttcatt
    15421 caggcggtta ttggcaggca ggtcggcaat caagagtcgg caggtcaggg ttccgatatc
    15481 gataccggcg agacgcttag aggtcattct gcatcgcgga tttcctgcat ctcggcttcc
    15541 agttttccgc tcgttgcctt cagttgatgg atgtctttga cgagccgagc aatctccgtg
    15601 tcatatagga cgcgttccac cggttcaccc gcctcacgga gagtgactgc gcgctcgccc
    15661 acatcacgat acaactcctt caactgctgg tcgattcttc tgacctcgag gcgcaggcgc
    15721 aacaattcgc tctcttcaag cgcgcggttg gccgcttggg cggttcccaa tcgcagcgtt
    15781 gtcaaaccgg ttttcaagtc atgcttgatt cgttgcagca gtcccatcga gctcttcctc
    15841 tcacctcttg tcgatcactt tctcacccac cagccctgcg gcggagagag ggtcgtccac
    15901 acccggctca accatgcggt cgaactgcgc gacggcgagc attgttcggt ggtccgatca
    15961 cggacggtgc aaacgcaccg agtggcacag ccgagcactg gcgaagcagt acggtcgaat
    16021 ccgggcaccg agcatacggt aatccatcca tctatgccct caacttatcg accccaactc
    16081 caattttttc tgccacttcc gcagcatcgc ctccttgatg ccttgatgag cggacgcagt
    16141 gagctgtgga tcctgcttca acaaggagaa ggcttccagc ctggcctggt gcaggaggtc
    16201 gccatcccgc accatgttgg cgacgcggaa ttcgggcatt ccccattggc gaaaaccaaa
    16261 aaattcccct ggccctcgaa tccgtaaatc ctcttccgcg atcacgaatc cgtcgttcga
    16321 acgcaccaat gcctcgagcc gctctttgac cagtgacgcc gactcttgat tcccggtagg
    16381 acgcttaccg ggcaacgtct tgttgcgtcc cattgtagcc gccatgagca ggcaatagga
    16441 ttggtaacca gcgcgcccca cacgtcctcg caattgatgc agctgggcaa gcccgaatcg
    16501 ttcggcatgc tcgatcatca tgacggtcgc attcggcacg tcgacaccga cctcaatgac
    16561 cgtcgtcgcc acaagcacct gaatcttccc ttccgtaaag tcggtcatga ctgcttcttt
    16621 gtccgcttgc ttcatccggc catgcaggac gccaacccga aactctttga gttctccctc
    16681 ctgcaatcgt tccgctccct gaatggcggc ctgcaaatcc gttttctccg actcctcgac
    16741 gagcggatac accacgtaca cttgccgttt gttctgcagc tcgtcccgga gaatctggta
    16801 agcgcggcgc cgctgcgcct cgccgaacag aaacgtgcgc accggcttcc gcccgggagg
    16861 cagcgtgtgg ataatcgaca cgtccaggtc cccatagacg gtcatcgcca gcgtccgagg
    16921 aatgggggtg gcggtcaata cgaccacgtc cggtccgtag cctttctcaa tcagggtttt
    16981 gcgttgcagc acgccgaact tatgttgttc atccacgacc gccaacccca aacttttgaa
    17041 tgcgacgcct ttttggatca aggcgtgcgt gccgatagcg acttggatac gcccagaggc
    17101 gaggtgtgcg atttggtttt ttttcgtcgc cgctttgtca ctgccacgaa gcaacgccgc
    17161 gttgacacca agactgtgta aaagagggct gagattccgg tagtgctgtt cagcgagaat
    17221 ttcagtgggg gccatcagtg ccgcctgata cccggatcca caggcgagga cgatcgcgtg
    17281 caaggcgaca accgttttac ccgatccgac gtcgccttgc accaagcgat tcatcggacg
    17341 cggcgcgatc atgtcgcgga atatttcccg gatgacttgg tcctgagcgg tggtcagcgt
    17401 aaaggggaga acacgtccca gcttgtccaa aagtggagtg cgaggattaa aatgcagcct
    17461 cttcttttcg acttggacgg accgttgtct cgcagccaac gccatctgca gcaaaaacag
    17521 ttcctcgaac gccagtcggc gatggacggg tgattttccc agttccagcg cggccaggtc
    17581 ggcgcccgcc ttcgggaaat ggatctggac gaccgcttcc tgaacaggca gcaaccgttg
    17641 gcgggcgcgg ataggcaccg gtaggatatc ttgcagatcg gccgcatgtt cgtctaccaa
    17701 tcctctcatt agcacgcgca tctgacgaga cgtccatcct ttggtttcat ggtaaatggg
    17761 gacaatgcgt cccacgtgca aggtggactc ggtgccttct ccgacgactt cgtactgcga
    17821 gacgtccatc ctcgggacca tccagccctg cctgcccgca atgacacgcc cgctcatcat
    17881 gatcgacttc ccgacggcaa atatctgttc cagatacggt tgattgaaaa ataccgcttg
    17941 caggcgccca gtttgatcct ccaagcctat ttccaagacg gtaaagcgcc tgttcctcgt
    18001 ccgcttggct gaattcttga ccaccgtgcc gcaaaccgct gtcaccatgc ccggaaccaa
    18061 ttggccgatg ggggtcatga ccgaacgatc ttcatagcgc cacggcacag tccagagcgc
    18121 atcttcgacg gtctcgatac ctaaccgttg caaggtcgag gcgcgttgtg gtccaacgcc
    18181 cttcgcaaat ctgatcggca cggtccagag atctcgtgtt gatttggaag gtgccggttg
    18241 cgttacagtc agagcctccg gctcctccca aacccggccc tgatcttccg tcgcctcccg
    18301 caacgtcctc aaaataccgg cagcctcccg taaccggcgc tgctgttcct cgagagtgaa
    18361 tcgctgttgg tcctcctcga acagtgcgcg cagtttctgc agcgcggcct caatagattt
    18421 cggatagacc tgatgagaca gcgcatgaag aatttgcgag gagacgtagg tactgaggtt
    18481 cttgacggtt ggcaggcggg caaacgcgtc gcgactggcg aattcgattg gacgtgcgag
    18541 acgatccaac cattgctgaa actgggccat ggcttcagcc gaccggtgaa cgaccattgc
    18601 tgtgcgttga tgacgtgaat tgaagcagta tcgccggact agaacttgct gtaaggatag
    18661 ccaaaaggcc cacacgtctc actcgcccaa ccccgacccg ccagaacccg tccctccctt
    18721 gcgaggcagc ggcatctgcg actacggcat cattctcttt caggggacga ggcgagaacg
    18781 aggcttggtg aaaagcgcgc ctcgacgtgc ctggctgggg gggcgagaag agcggctttt
    18841 tcaatagtct gctcggctcg gaggagcgcc acattgggcg agatggtatc acagcccctc
    18901 tccccgctca acgagagtcg tctgtggctg cgatcccgaa ccctgtagaa ttccttactt
    18961 tcctttggca gcgttcagcc tggtgctaga ctgcgatcga ggtgaaaggc gcccatgtac
    19021 cgccggaata tttccaacgt cctctatccg attgtggccg gactcggatt ggtcattgtc
    19081 tatcagtcga tcgtcaagcc gacgtatatt tctccgctga cggcatcgca ccgggagcag
    19141 ggacctctag aaacgggaac agtgtctcga gagccgttgc tggaaccaat gattgaggag
    19201 cctcacgcca ctgctcccaa acaattgcgc gtcctcgagg cgcctgccgt gccggagtcc
    19261 aaacaccggg gatcgttgga aaccattctg gaggaaatcg aaaaaggcaa cctcaaagaa
    19321 gctgaagcct cactcatcca ccttcccacg acgctgacgg acgattcccg tatctacgca
    19381 gccgtcctct ggaacaatct gggcatcgaa caagaaaaac tcgagggcac gaagggctcg
    19441 ctcaagacat tcaaaaaagc cgcatccctg gatccgaaga atcccaccat ccagttaaat
    19501 ctggctcatg cctattggga gcagcgcgac ccagccatga gccaggattt cttggagcgc
    19561 ctgatcgccc tcgcaccgca ggagcctttt ccacacgtgg ctttagccga gttacttcat
    19621 gaacggggtc gtctgagtga agcggcgcgg cacctcgatc aggctgctga acgggtggca
    19681 agggatccct ccatgcaatc gtacctccag acggtgtcat cgaaggtgcg gcgcaccgac
    19741 gaaatggaaa gccggctgtc gacccgcgag ggcgctcatt tcacggtcaa gtacgacgga
    19801 gagacggatc aagccacgtg gacggtggtg ctggatgtac tggaggaggc ctaccgcgag
    19861 atcggtcaga agtttggtca cttccctgca aagaccattg tagtggtctt gcatccaaga
    19921 gcgacctttc aaggggcaac gggaagtccc gcctgggccg atggactgtt tgatccggtg
    19981 ctcgggcgta ttcatgtacc gacacaaagt gcgctggagg atcgcgcctg gctcaggcgg
    20041 gtgcttcggc atgaatttgt tcatgcactg ctgcacgatc aacaaggact caaccaccat
    20101 gccattccca cctggctcaa cgaaggactg gccatgcagc tttccaatga ccattggacg
    20161 gacctggacc agcttcatca acaaaagctg tcgattattc ccttaacggc gttagaaggt
    20221 agttgggggg aattgcagag tgaggaggcg tcggtggcct atctcgaggc cgactccgca
    20281 gcaggctact taatcactcg ctatggcatg catgaggtgc agcagttgct ggccaggctt
    20341 aagcaaaagc aaagcttgcc cgcggcgatt catgctcaac tgtccttgtc ctacgagcag
    20401 cttcaatccc gttggatagc gcatctgcag gagagacgac cgaggggata ggtgatcggc
    20461 caccctgagg cgttttaccc gccgtttcat ccgagcgatc cggcacggca gactgttcga
    20521 tttcctcttc aggaacgacc ttgtcttccc acaacaatcg gcggccggcc tgatctggca
    20581 tcatgatccg gaaatcgata tctcgaggcg cggcacccgt gacgccgatg acactccggc
    20641 cgatgcgctc gatccgaact gtggacgcct cggtggaaaa ttcatcggac cacatgagtt
    20701 gtcccgtgtc gggatgacgc gctcgcagca cgaagtacgg ctgtccggtc cccttcgtag
    20761 agctcgcaat cctcgtcact gcggctcgtg gctgaacatg cgccataact tgatgggtgg
    20821 ctgcttcggg atgatctccg atggtcagat tcaggcggcc ttcccataga aaatgtccgt
    20881 tcgcggcatc atagacgcgc agagtgaact cggaaagact ccctgcgcca agcccgaccc
    20941 ctcctgcaaa gatgcgagtt ttggggtggc tggtgggtgg tccgtcatct ttgatgtcga
    21001 gttcgtacgt gtctgctgtg aggacttccc cagactgggc gtcgtaggtc ttgacggtga
    21061 tggatgaggt ctcgtcgtcc tgaaagccaa atcccgccgc aacgaccgtc cggatatgcc
    21121 cggctgccat ggacggactg tccgttgtcc cagccgtagc gggaacggtt cccacctcaa
    21181 agaatgtgag gacaatcgcg agagaataca ggtgctgcag gcaagttcga atggatagac
    21241 gggagataga ccgcatgggt gacgctggtc tccttacctc gaccgactat gacaggatcc
    21301 ctcgcagggt gacattcctc gctgtgggga gacaatcccc caaaagtggg agtctaatcc
    21361 agcctcttca aaggcacttc gccgaagaga cggtatcgat agcgtgctac gaggcgatag
    21421 gcaaggtagg caaacggctt gacaagtcgg agagaaagga acttggccag tggatgcccc
    21481 cccttcaatc caggcagcaa aggaagaaac gcatcgagtc ctgtggcaat cgttcgatca
    21541 ggatggacga gaaacgcggc aggtggccgg cccggccggt agtgagctcc cagcacttgc
    21601 ttggcttctt cgctctgata cgggatcaaa cgaacttcgt cggcgtggct ctcccgacgg
    21661 agttgctcca ggccgttttt ggccgtcacg cacagccggc attggccgtc atagacaagc
    21721 acgcattggc ctgactgttt cgagaagggt tctgtcatcg gcggtcagcg accccattgc
    21781 ccgctcaata cgcgatcgcc tatgatccgc tgatgcgcca tcgggcgatc gtggcggtca
    21841 cgcttctgtc tgtcagcgtc attggcgtat caaccatagg gttggcgttc tcgagcgaac
    21901 tgccaccaac gacttatgtc ctgtggtggc tctttctcgt gttagtaccg ctcgttctgg
    21961 cctgctttgt catggttggg tggacttggc ccgccatggc gagcgtggtc tatggcacca
    22021 ttggactggc tctcgatctg tccacgctcg cgccgctcgt cggaggactg cacgaatcgg
    22081 gtctcaaggt cgtcctcagc ctatttagcg ggtgtgcgaa ttttttgctg atcgtgttcg
    22141 gtggccgtgc cttttggagc gccctcgaag gacggcggcc tccagaatcc cgccctccca
    22201 gtcctccttc cccttcttca tcctgaccgg cctcaccgaa tcgaatcctg cgtccttgag
    22261 ccaggcgctc gtttcggcga ccgagtaggt atttcctccc tcagtgaaga gcagcatgga
    22321 cacggcaaaa agactggctt cttcgggaaa gagtccctcc cggtcgtgca ggaaggcatc
    22381 ttggatcacg agacggccgc catcggccag agcctccttg gcgcggcgaa acacatcctg
    22441 attctgcgcg ggcgaataaa tgtgcaggac attcgaatac caaatcacat cgaatggacc
    22501 tggaatatcg tcggtcaaca aatccagcgg caagtaagac agccgggcac cggccttgtg
    22561 ggtcgctgca atgtcctttg ccacttccaa ggccgcagga cgatcgcaca cggtcgcacg
    22621 aaggcgcgga tgcttcgcca agaaggccat agcataggtt cctggccctc ctcccaaatc
    22681 gagtaacgta ttgacccctt tcaagggaag ttgggcggca atgatgggcg ccgtttccaa
    22741 cgtgcgatga tgcatggccc acgtgaaccg ccgacggtaa tccggctctt ccggttcgtc
    22801 cttgtccaga ggctcaccgc tcttgaccga ctcaagcagc cgcagccaat cagaccagtg
    22861 attcgtgatg aggtcgagat aactgcctcg gtagttggga tcatcggcat tcaaggcagt
    22921 tgccgccaga gggctgttgc gatacctatt gccgtgtttg tacagcaatc ccgccatggc
    22981 aagatttcgg cagaggatgg acagtccccg ctcgctcact ttcagctccc gggcgagatc
    23041 ggggagcagc caggtggcct tcccaatcac cgtaaacagc ttaagctcaa gggcagccag
    23101 gatgacacgc ggcaagcgat acgccgacaa ggcgtcccgg aattcgtcga aggttgtaat
    23161 cggctgtctc atgccgctcc cccgatctgt cggcagcacc agcgcatgag gtcctgaacc
    23221 ttggacaagt tctcgagatc aacctggcgc tcaaagctga ccgccacgta ttcctcattc
    23281 atctcggttt gctcggaaaa accggactgg agcagcaagg gacggtattt tgagacagcc
    23341 ggttggatga aatacttcgc ctgcttggcc acttcgtccg tgcccaaatc ttccggcgta
    23401 ccctccgaca tcaagctcat cacgtcgtcc atcgccacgc catactgaca gagcagcttg
    23461 ccttccggtg tgatctcgag cagccagccg tcttggccaa gatcgagagc gaggggaccg
    23521 tctggctcga gctcgtagag ctgcggaaag gccttcgaca acgtgctgcg tgcctcggtc
    23581 catcgaggtg acttcctgtc attcatccgt gagactcctt acgtgcggcc gtcgtcgcgt
    23641 tctcggcggc aagccgttga cggagaccct cgagccgttt cgtgttctct ccgagtttag
    23701 gcaaatgata cttgcggtcc atctccacga cccgctcgag caattgcgcc gcttcccgga
    23761 atcgacccat cgcctcatag cagtcggcaa gaacataccg agctccaccg gtacgcaatt
    23821 catcctgcga gcgttcggca atagactggc tggtctgaca acacgcgatc gcgtcgtcaa
    23881 agcgttgttg cagcaaaaag gtccgcgcca acatgcgcca ggcatccgcc acgcctccat
    23941 cgttgcccag tctccgcatc aagaccaacg acttctcata atgggcaatc gcctcgttaa
    24001 actctccttt ttccctcgca atcaatccta aatcagaaaa caggacagct ttcgaggtct
    24061 cgtcctgcgt cttggtcatg agatcgagtg cttccaagta atacgctcgg gcccggtccc
    24121 actctcctgc atctgcgcgc agatttccca agttggccag ggtggtcccg atgccctttt
    24181 catcgccgaa aattttttgg agctcgagaa cttcttggta ataattctgc gccgcgtcgc
    24241 ggcggccgct cacggcacaa atattaccca aattgcctag cgttgccgcc agcgcacgtt
    24301 ggtcaccggt gaggcggtcg cattcaaggg ccttggcgta gcacgtatac gcctcggtgt
    24361 agtgaccgcg gccgaaatgc tcattgccct ggcggttcaa ttcttcggat agaccatttt
    24421 tcagtgtcat gacttgccgg agccttgtct tgcgagactc tcagcccatc tcctttcggc
    24481 atccagaggg tgctgcctca tgatccatgc tcggccagga ctttttcaac ggccgccttg
    24541 gcgcctgtca tgatggatgt cccatcgccc accagcatgg cgtcgaaatt atacttgagg
    24601 agacgtcgga gcccttcttt cgctttgacg acatcatggt acttttcttc cggcaacatg
    24661 gtgagcgctc caggaggctt gccaatcaat gcatcaccga cgatgagcac ccccttgccc
    24721 tgctgaatga acagggccga ttcgccggcc gacttttgat ccaagagatg gatgacccag
    24781 atcccgccgg ggaggagctc gccgtctttg aatgtcctag tcggcttgag atccatctgt
    24841 ggagcatcag cctccggcag gaacaactgg cacccgaaat ctctccgaca cgcttgagcc
    24901 tctcgtgcgt gatcacgatt cgtcacgata atgtagtcga gctggccctg cctacggatc
    24961 actgtcgtgg cttctgccgt caagggcggc gggtcaacaa ggatcctatg ctctccgacg
    25021 gtaagaaaca ggccattgaa attgagctgc ttctcttcgg aaaaccagga ccattgccag
    25081 atacctggaa aaattggctt catgaaagat gttcaccgat aggccgattc aaggacacag
    25141 gcgtttcata aggctgcaac ggcgtctttg atcgtcttcg taacttttgt gaggatgccg
    25201 gattcatgcg gcagatcgat gatgtcgtcc tccggaatcg tgatgaattt tcgattcgct
    25261 cccttggtca gggaaatcag aaacatgctg ttcgagggcg ttacagggat cacgacttgc
    25321 acctgctgat ctatgtctct cacgagtgca agaaacttct gttttccctc ttccatctca
    25381 tccattctgt cttttccttt tcttcgtcat cctcaaacct ctgcgcgatg gctgggaact
    25441 ctcaacctgc gcacttgacc aagcacctac ctcaacttac caatttaata gtgttgggtg
    25501 tacagcggtc ggcgagcaga gagagtccca gtcgtcgcgc tcttcacatc ttgttaccgc
    25561 cttctgccgc caacaacttc tcaacttcag caataatgac gtctgaaccg gccagctcca
    25621 tattgccgac ccgctgccag cggatgacgc cctgctgatc gatgacatac acattgggaa
    25681 tgaacttccc gccgccgtac attttgtctg taaccttgcc gggatccaga agatacggat
    25741 acgtcacctt cacagggaat gcggaaagaa actcaccgac gtcctttttg gaatttccgg
    25801 atgaattcac gccgatgact gccacattct taccctgtgt cgcctcgtac accttttgga
    25861 gattcgtgcc ctgcatcatg cagggatcgc agatgtgaaa gaggccaagg actactacct
    25921 ttcccttgtt ggcatccaag gagaccggct cgccggtaat ggcattcagg gcaaacgaag
    25981 gcgctcgctc cccgacctta aaaaaccccg ctgccaacag cagatgggcg gtgagcactg
    26041 gccataaaag gacggttaag atgacccaac gacgtttcat gcgaacctcc attctgaaac
    26101 cctgcaggag tgtcgagtgc cgactgccta gccatcttct cgccgggtat cctaccatgc
    26161 gatttttttc tggagcaatg tcggaagttg ggtttcccag cagaacaatc ctgaccggcc
    26221 taaccgcggt tagtgtcgtt cgagcaaggg ggtaagaagg cggggcagat ctaagacgtc
    26281 cctgccaaaa tgcaagcgat agcaggcagc ccgttgcacg agcttcgaaa gcacctgaaa
    26341 ctcctgtgcg gcggtgtcgg catcgtgctt gtgggcttca cgccgaacaa gcgcttcgag
    26401 cgccaggctt ttagccaggg gctcgaggca actatgagat atgttcgtga cttgaggaaa
    26461 cagaatcatg gccggctcgc atgaccgtcc ccgtgacccc ggatacacat cttccggatg
    26521 aaatgatttt ctgtatgccc cctgccgtaa gagtctgagc ccggcggtcc gtaactcggg
    26581 aaccatctcg atggtgtggt ccgtcacatc gatggtcgga gacgcccccg gcaacagttg
    26641 cattggacca tgatcgcgta acagcgaaat atcgtcaccc aggtagcgat atccggaacg
    26701 cagtaaagac agcatggcgg tcgtctttcc gcatccattg gggccgaaaa gcaccacgcc
    26761 tttgcccttc cattctagtg ccgccgcagg cagtgggaat gtatttttcc cctttagcaa
    26821 ctcgatcatc gtgtaatgga aaaaggtttc cagtcggtca ggcgccatgg tttcaggaca
    26881 ataaaaatgg ccggccgccg cactgcggtt tcggtcgatc agcagcacgc cgtgatcccg
    26941 gacatccacc accagacgct ccccgtcgcg atacacgtcg cattgccaga gagcgcgtag
    27001 ggaattgccc agcgctggca tggtaccgga gaatacccgc ctggcaccgg gagaaagcgg
    27061 gaccggaacg gcctcgcggc tgctgacgtg ctcaaagtgg gcggtcaatg agacgtcccc
    27121 tttcacgtcc ccactgtcga cagtgcgaac ccatgcggcg atcgaagacg acaaggacgc
    27181 cgaggagctg cagaagcgga acggaatgcc atgaaatgag aaccggtgtt cacgtgaatc
    27241 gccggtgatc gtatgctcgg ctctgagacc catcacaagc tgcgacacgt agcctcctga
    27301 cagaagactc tatcgacctg ccccatccgg ttgatgaagg agtctgtcat ggctccattg
    27361 cacaacacac gcgatcatct gttcacgcaa atcaccaaca tgccattcca agcgtgcaga
    27421 gatcgtatga tggatttgct caagagtagc cgttcccgta cagtgttccc acactgccgt
    27481 tccgaccgca ttcagccgga aggattggcc cgtgaccaga tccaacagca cagtctcacc
    27541 ctccaggacc acgccccgga cattcggatt cttcgccggc gcgaggtgca tgattctgct
    27601 ccaatctggt gtatggtctc cgtcgttcgg gaggactggt gccgtggttt ggaggccttc
    27661 ttcctccatg aacggcaaca aggggggcgt cgacctgtgc ctgggcggcc tgcaccacat
    27721 acggagtctc tccggcggct cttgaagtcc cacactaatc ctctccaggc tgtattcaca
    27781 gctccctaaa agctgggcgc acgcccctac tttcgtagag gccactgtgg ggtgagaaga
    27841 aatatggttc ttacgaagag cgctcgaaaa ggccgagttg gaattcttca gcccgcgtaa
    27901 aggaaatggg gtactgttcg gtgaagcaag cgttgcagta atgatggggg gcaccagggg
    27961 cggatttcag catcccgtcg agactgagat aggctaagct gtccgccgtg atgtacttgc
    28021 gtatttcctc ggcggaatga ctggaagcga tgagttcctt tttggtcggc gtgtcgatcc
    28081 cgtagaagca tggtgaaatg atcggtggag aactgatccg catgtgcact tcctttgcac
    28141 cggcctgacg gatcatcttc acgatcttgc ggctggtggt cccccgaacc agggaatcgt
    28201 caacgacgac gacccgcttg ccgtggagta cctccggcac cgcattgagc ttgaccttga
    28261 ccccgaaatg cctgatggac tgctccggtt cgatgaacgt gcgtccgaca taatggtttc
    28321 ggatcaatcc ggtctcaaac ggaatgcccg ccccttcgct ataccccaac gcggccggca
    28381 cccctgagtc cgggacgggt atcacgaggt cggccggcac ccatgactcc tgagccaatt
    28441 gacgtcccaa cgcctttcga gtcgcataga ctgcgttcgc gccgaaaatt ttgctatcag
    28501 gcctggcgaa atagacgtac tcgaagatgc acattgccgg ctgcactgcc gaaaacggtt
    28561 tgtaactcgt cacaccattt tcattgagga cgacaagttc acccggttcg atttcccgga
    28621 cgatttccgc atccaacaaa tcgaacgcgc aggtctccga ggcgaccaag tacgtgtccc
    28681 tgagcttccc aagcgatagt ggcctgaagc catacggatc ccgcgctgcg atgatccctt
    28741 cgtccgtcag caggacaacg gaaaatgcgc cgcggacttg gctcagagcg tcgatgacgc
    28801 gggccaacaa ggtgtcggct cgtgaatggg cgatgaggtg aatgatgact tcgctgtccg
    28861 acgttgattg gaagatcgcg ccgtacgctt cgagttcatt gcgaagcacc tgggcattga
    28921 tcagattgcc gttgtgtgcc aacgcaagat tgccgaaagc gaaattcacc gtcagcggtt
    28981 gaacgttctt gaggtcctgg ccgccggccg tcgaatatcg attgtggccg atcgccatcg
    29041 aaccggtgag attcttcagg gtctgcgtgt tgtagatgtc ggccacgagg cccatgccct
    29101 tgtgcacatg aaagtgatcg ccgtccgcag acacgatgcc cgaggcctct tgcccgcggt
    29161 gttgcagcgc gtagagacca agatacgtga gattcgccgc ttccttgtgt ccgtaaatgc
    29221 cgaacacggc gcactcgtcg tgaaacttat cggacccggc gatgggaagt tccttacgca
    29281 taggcaacgc tattcatggc gtaactgttc ttcgagcgca tgagcccaac ggtcccagac
    29341 tcggtcgatc ggaagatcga tgcgacagcc ttcgttcatt cggcccttcg ccacgtcaat
    29401 gatgaaacga tctccgccga cggtgccgat gcgaccggcc ggtattcctg catcagctgc
    29461 acgactcaac aaggcctcga ccgtatccgg cctcatagag aggatgacgc gagacgaact
    29521 ttcaccgaac aacagagcgt cccgccgcaa cgaaccgagc tccaatttta ccacagcgcc
    29581 acgccttaca tccggacccg acacacagca ttccgccaac gcaacagcca ggcccccgtc
    29641 cgagcagtcg tgcgcagact cgacgagccc ttcccgaatg accgttaaga caaaatcctg
    29701 caacgccttt tcagtttcga ggttcaacaa tggaggcgag ccctgttccc gatgctgcat
    29761 gaccttgagg tactcactgc cgcccaggtc atccctcgtc ttaccaagga gaataatggc
    29821 atcttcatca tggcgaaacc actgagtcat ggcctgctcg gccttatcga tcaatccgac
    29881 cataccgagc atcggcgtgg gataaattga gaggccgttt gtctcgttat aaaaactcac
    29941 gttcccgctc acgatgggaa tttggaaatg ctcgcacgcg tcctttaacc cttcgatcgc
    30001 caggacgaac tgccacataa tgtccggccg ctcaggattt ccgaaattga gacaatctgt
    30061 cagcccgatc ggctcagcac cggagcagac cagattgcgc gccgcctcgg caacagccaa
    30121 gcgggttcct tcatacggat gcaacagaca atagcggcca ttgcaatcca ccgtcatggc
    30181 caccgctttg ttcgttccct tgatgcggac gaccgcggcg tcggaaccgg gtcggaccag
    30241 ggtattcgtc cgcaccatgt ggtcatattg ctcatacacc caccgcttac tcgcgatcgt
    30301 cggagaagcc aatacggaca acaacgccgc attcgcgtcc ttcacgtcag gcatcgaatc
    30361 gtaattcagg ttggtcagca tctcctggta gggcggcgga gagtacggcc gctcatatct
    30421 cggtccgtcg tccgccaggg atttagcggg gatctctgcc acgacctttc cctgatcgat
    30481 gacccgcaag agaccgtcgc ttgtcacttt accgacgacc gccacatcga ggtcccactt
    30541 ccggcaaatc cggatgcact cttcctcttt tccggcttgt gcaaccatca acatgcgctc
    30601 ttgcgattcg gacagcatca attcataggg ggtcatgccc ggctcgcgtc gcggcacatg
    30661 agtgagatcc aattccatgc cgttgccggc gcgcgacgcc atctcacagg acgagctcgt
    30721 cagaccggcg gctcccatgt cctggatacc gacaaggaga tcattcgcca tcaattccag
    30781 acaggcttcc agcagtaatt tttcttgaaa gggatcccct acttggacgg tcggccgttt
    30841 ctggtcggag tcatcgtcga aggcgtctga cgccatcgtt gccccgtgaa tgccgtctcg
    30901 acccgtcttt gatccgaaat aaatgaccgg attcccgacc cctgcagcgg tgccgcggaa
    30961 gatcttatcc ttatgtgcaa taccgaggca gaaggcgttc acgagggggt tcagcgcgta
    31021 gatgtcgtcg aagcgtactt cgccgccgac agttgggact cccatgcagt tcccataacc
    31081 ggcaataccc gcaacaacac ccctcataag atggcggttc ttggcgctgt ccaggctgcc
    31141 gaaacggaga gagtcgagga gcgcgatcgg cctggcgccc atcgtgaata catcacggag
    31201 gattccaccg actcccgtgg cggcaccctg atagggctca atgaaggacg ggtggttgtg
    31261 cgactccatc ttaaaaacca cgcacaagcc gtcaccgata tcgacagcgc cggcattttc
    31321 gccaggcccc tgtacgacac gcggtcctgt cgtgggcagc ttcttgagat gcaccctgga
    31381 gcttttatag gagcaatgct cggaccacat gaccgagaac atgcccagtt cagtcagatt
    31441 gggctcgcgt cccaaaatgt cgacgatctt cttgaactcg tcctccgtca gattgtgctg
    31501 tgcgaggagg tctttgacgt cagccgtgtt ggtattcata gatcctatac attacagtga
    31561 acaacacgtt caccgtgact tactcgcagg tcatcacgcg cccaccattt ttaacggccg
    31621 tttcttcagc gactccagca tcgatgtgaa gatcagccgt ccgtcctcat tgcccatcag
    31681 ttcctctgcg gagcgctcgg gatggggcat catgccaagc acgtttccct cggcgctgcg
    31741 aatgccggca atattgtcga gcgagccgtt gggattggct tccagcgtca cacgcccgtc
    31801 aggggtgcaa taccgaaaca cgatttgagc gttggcctgt aaccccgcca gcatcaccgg
    31861 atcggtgtaa tagtttcctt ccgcatgggc aatcggaatc ttcagcacct gaccaggctt
    31921 gcacgcgccc gtgaatggcg tggccgcgtt ctccacacgc acatgcacat ctttgcagac
    31981 aaagtggagt gaacggttcc gcaacatggc accgggcaac agtccggctt ccagaagaat
    32041 ctggaacccg ttgcagacac ccatgaccaa gccaccgctt ttggcgaact ctttgatggc
    32101 ggacatgacc ggtgaaaacc tggcaatagc ccccgtgcgg agataatctc catacgaaaa
    32161 gcctcccggc agaatgacgc catccagacc gaccaaggac gtctccttgt gccagaccat
    32221 cgtcacattc tgggccagca cgtccttgaa gacatgctcg caatcgtgat cacaattcga
    32281 gcccggaaaa acgatcacgc cgatgttcat gtctacacca attcgtaccg gtattcttcg
    32341 atgatggtgt tggcgagaag cttttcgcac atggacttca cctgagcttc cgccttcgcc
    32401 ttatctgttt cgctgacatc caattccata tacttaccga cccgcacgtt cgcggcgttt
    32461 ttgaagccga gagaatcaag cgcatgctcg atcgccttgc cttgcggatc cagaatgcct
    32521 tgcttcaacg tcacatgaat cttcgccttc acgttttagt ctcctgctca cccgattgct
    32581 tgtgtaaacc atcgacatat cgtcgaatct tgaagagtac tgcgaccgtc gcgatccctc
    32641 ccacggcaag cgcggcaaaa gcccagcgcc agggtgaatc gtccaaccgg tacgccatca
    32701 tcgccaccac ccccgcccag accaacaccg ccgccaccaa gccataattc agaaacgctt
    32761 tcagcatttg cctacttctt tgtctccctt ctcctcaaaa gcgggcggag ttctgagcct
    32821 tgttccgcga tgtcacggaa actcgggtca ccgcattcgt tgagcggagc ggtccgccat
    32881 gcggacggct cgcgatgttc gaagttccgt tcgagcgtag ctttgaacgg aacaagtttc
    32941 gcggggcgag cgatgcgaaa cgtcgtgcgg tcgagttgtc gtgtgtgagc gggcaaggct
    33001 cagcctccgc tacccgcata cccgcttcag cacctcctga tacgcttcct ctatcttgcc
    33061 caagtccttc cgaaaccggt ctttgtccat cgact
//
LOCUS       DASSCQ010000234         6349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_1814328, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000234 DASSCQ010000000
VERSION     DASSCQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814328"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..840
                     /locus_tag="VFH05_14730"
     CDS             70..840
                     /locus_tag="VFH05_14730"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6679528.1"
                     /translation="MARKGGKRYRYRTNTQDIDWQEERADASQNIGEMQRLFSAIAGV
                     GLIMGGLRRRSLGGGALAIGGVSLLYRAATGYCPALGAMGIDMGGMQDTNRLGRRKVH
                     SGQATKVRRSIDINRPAAELYRFWRALDNLPRIMSHLKSVDVISDRLSHWVVNTLPGL
                     PTVEWDAEIINDVENERIGWRSLNESDVDHAGSVEFETSGEGSTRVTVTLQYEPLAGR
                     LGTAVAKLIGQDPELKIADDLQRFKESMEAGTYSQTNR"
     gene            900..1418
                     /locus_tag="VFH05_14735"
     CDS             900..1418
                     /locus_tag="VFH05_14735"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET6679529.1"
                     /translation="MPQVTLTGHPLHPQLIVMPAGLLPFSLVLDALHAKTRDQSYADA
                     AYYTMMGGFVGGLVAGAAGAADYSTIPADSKAKEIGRIHGVMNVALLALTGVNLWMRR
                     RRRSPGTLPILLSLLGTAGLTVSAWYGGHLVYHHGMRVRGTDLAGGPEDLKLPGDERM
                     AHMLASTAGDEA"
     gene            1415..1969
                     /locus_tag="VFH05_14740"
     CDS             1415..1969
                     /locus_tag="VFH05_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET6679530.1"
                     /translation="MTGADPGFHVKGDATVGRAEPLRPEDHVIGPDSAPVTVIAYCDF
                     ECPYCGRAFAMLRDLRARLKGQMRFVFRHFPLIDKHPLAQQAAEAAEAAASQGQFWPM
                     HDALFEHQDALERDDLYRYADAAQLDLARFKEDLDRRAHLSRVMHDVMSGRRSGVTGT
                     PTFFLNEALVPEDERLEDLVRRAA"
     gene            2022..2885
                     /locus_tag="VFH05_14745"
     CDS             2022..2885
                     /locus_tag="VFH05_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6679531.1"
                     /translation="MKTDTRLILPAKEGVSSTEEGSIQFIGNATVLIRYGGMTVLTDP
                     TFIHRHEQVSIGYGMHSTRLTDPAIEIGDLPPLDLILLSHFHGDHFDQVAERELDKSI
                     PIITNREAAKELQQRGFRYSKSIDTWSSVSVEKGDMRMRITSMPGRHGPPLSELVMPE
                     VMGSMVEFQPIGGSSFRLYITGDTLIFDDLKEIPRRYRDIDLALLHLGGTRVLGLLVT
                     MDAKQGVELVQLVRPRQAIPIHYNDYDVFTSSLSDFQHEVEAAGLRERIHYLSHGDTY
                     RFRPSSKRAAA"
     gene            2987..3172
                     /locus_tag="VFH05_14750"
     CDS             2987..3172
                     /locus_tag="VFH05_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679532.1"
                     /translation="MKPRKRASNKPRKAKTSELERDEEYRAVIEPDPLLKEVGEANER
                     IADPAERERVVPSRPCK"
     gene            3253..4989
                     /locus_tag="VFH05_14755"
     CDS             3253..4989
                     /locus_tag="VFH05_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET6679533.1"
                     /translation="MYTLGINAAFHDPAACLVRDGVVIAAAEEERFTRIKHGKRPIPF
                     TTYELPFHAVEFCVRQAGIRLTDVDHVAYSFDPFLLLGRHAGASQIAIPLHGTGSESN
                     EWESPWDPLALSAIVNAPRHLASGVPHHLGERLSGLAESGPARWHYVPHHVAHAASAF
                     HASPYETAAVMTLDGRGERATTSYHVGKGQDLTEHGAVTMPHSLGLLYEQVTSYLGYL
                     HSSDEYKVMALASFGKPEYVDILRKSVRLLGGGQYVVEPLNLIELCGPPRARGGALEQ
                     RHYNLARSLQQVLEEITLDLASWLHESTGEPRLCLAGGVALNCVLNARIRSASPFKEV
                     WVQPAAGDAGTALGAALWIDAQVCPTSGARRSTMEHVFLGPAFEDQEIEAVLRRAKVS
                     YRRLHHIPEDTAELLERDLIVGWFQGAMEFGPRALGARSILASPIDPAMQDRLNDIKD
                     REDFRPVAPVVLEEDAADWFADARVSPFMLFVFDVKSDKAMRIPAVRHVDGTARVQTI
                     NRTQHPLYYDLINAFKRRTGVPVLVNTSFNVRGEPIVCTPRDALSAFFSSPLDALVIG
                     PFLVTKAAAKTP"
     gene            4986..5954
                     /locus_tag="VFH05_14760"
     CDS             4986..5954
                     /locus_tag="VFH05_14760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6679534.1"
                     /translation="MKISVVIATLNRPVLLDRCLAALSRQTLPPTAYEVVVVDDGPHR
                     ATEAVVRRWSGRSPGFEVVYRCSGQAHGPAAARNVGWRAGRYPLIAFTDDDCVPEPGW
                     LAAGLQVFLRPEVGAVSGRILVPLPRKPTDWERNVAGLERAPCATANCFYRKTILEAL
                     GGFDERFTEAWREDSDLQFRALAHGVTIVHEPQARVLHPVRPAPWGISVSLQRQNRFN
                     ALLYKKHPDLYSCFNPHEPPWNYYVTVGSMLFGGIAALYGAVPMATALWVCWLIMASA
                     FCVRRLVGVTHRPSHVAEMIITSLIIPPYAVYWRLHGALKYRVAFF"
     gene            5994..>6349
                     /locus_tag="VFH05_14765"
     CDS             5994..>6349
                     /locus_tag="VFH05_14765"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HET6679535.1"
                     /translation="MSEDPKDNKREIDADQMIRDYEETAYTEEPEEAQTEIPWYEQAK
                     QDTSVPAEAVLTGGDIDAAWDQAAVGEETVGGSTPTPDQDIVDEIGRAVGVNYEDSEP
                     LDPEAKIERREENRWE"
BASE COUNT         1297 a         1794 c         1945 g         1313 t
ORIGIN      
        1 agactatccg atggcaaatg aagtcacgct attgcacggt caaccgttcg aatgtggagg
       61 gagtatttca tggcacggaa aggcggcaaa cgatatcgct atcggacgaa cacgcaggac
      121 attgattggc aggaagagag agccgatgcc tctcagaaca ttggcgagat gcagcggttg
      181 ttttcggcaa tcgccggtgt tggattaatc atgggcgggt tgcgccgtcg atccttgggc
      241 ggcggcgccc tggcaatcgg tggcgtcagt ctgctgtacc gagcggcgac gggatattgc
      301 ccggcactcg gcgccatggg gatcgacatg ggcggcatgc aggacaccaa ccggctgggc
      361 agacggaaag tacactcggg acaagcgacc aaagtcaggc gatcaatcga catcaatcgg
      421 ccggccgctg agctgtatcg cttctggcgc gcactcgata atcttccacg cattatgagt
      481 catctcaaat cagttgatgt catcagtgat cggttgtctc attgggtcgt gaacactctg
      541 cccggattac cgaccgtcga atgggacgcg gagatcatca atgatgtgga gaatgaacgg
      601 atcggttggc gatcgttgaa cgagtccgat gtcgatcacg ccggttccgt ggaattcgag
      661 accagcggag aaggaagcac ccgagtgacc gtgaccttac agtatgaacc gctggcaggc
      721 cggctcggaa cggcggtggc caaattgatt ggtcaggatc cggagctcaa gatcgctgat
      781 gacttacagc ggttcaagga atcgatggaa gccggaacct attcgcagac gaaccgctga
      841 tgtgtcaggg ctatcgctca tgccaacgcc gaatgtaaac ggataagaaa gggaaatgca
      901 tgccacaagt gacgctcaca ggacatccgt tgcatcccca gcttatcgtc atgccggccg
      961 gtctgcttcc gttcagtctg gtccttgatg cacttcacgc caaaacacgt gatcagtctt
     1021 atgccgatgc ggcctattac acgatgatgg gcggcttcgt tggcggactc gttgccggcg
     1081 cggcaggtgc agccgattac agcaccatcc cggcggacag caaggccaaa gagattggcc
     1141 gcatccatgg cgtgatgaat gtcgccttgc tggcgttaac cggcgtcaat ttatggatgc
     1201 gccgcagacg gcgctcgccg gggactcttc caatcctcct ctccctccta ggcacggctg
     1261 gcttgaccgt gtccgcctgg tacggcggcc acctcgtgta tcaccacggc atgagagtgc
     1321 gcggaacgga cctcgcaggt ggaccggaag atctcaaact tcctggcgat gagcgcatgg
     1381 ctcacatgct tgcttcaacc gccggagacg aagcgtgacc ggtgcagacc cgggcttcca
     1441 cgtaaaggga gacgccaccg tcggacgagc agaaccgttg cgaccggagg atcatgtcat
     1501 cggtcccgac tcggcgcctg tcacggtcat tgcgtactgc gattttgaat gcccctactg
     1561 cgggcgtgcg tttgcgatgc tcagggacct gcgtgcccga ctgaaaggtc agatgcgctt
     1621 cgtcttccgg cattttcctc tcatcgacaa gcatccactc gcccagcagg ccgccgaggc
     1681 tgccgaggcc gcggccagcc agggccaatt ctggccgatg catgacgcgt tgttcgagca
     1741 tcaagacgcg ttggagaggg atgatctgta ccgctatgca gatgccgcgc agctggattt
     1801 ggctcgcttc aaagaagact tggaccgtcg agcgcatctg tcgcgggtga tgcacgatgt
     1861 gatgtcgggg cggcggagcg gagtcaccgg tacaccgacg ttctttctta acgaggcgct
     1921 ggttccggag gatgagcgtt tggaagacct ggtgcgtcgg gccgcatgag gcgtgagcca
     1981 cgtaagatga tgaggatcca gctttctgga gataacacag catgaaaacc gatacgcgtc
     2041 tcatattacc tgccaaagaa ggagtctcct ctacggagga aggcagcatt cagtttatcg
     2101 gcaatgctac cgtgctgatc cgttatggcg ggatgacggt gctcacagat ccgaccttca
     2161 ttcaccgtca tgagcaggta tcgatcgggt acgggatgca cagcaccagg ttgaccgatc
     2221 cggcaatcga gatcggcgat cttcctccat tggatctaat cctcttgtcc cattttcacg
     2281 gcgaccactt cgatcaggtc gcagagcggg agctcgataa gtcaatcccg atcattacca
     2341 accgtgaagc cgcgaaggaa ctgcagcaac ggggttttcg atactcgaaa tccatcgaca
     2401 cctggtcctc cgtctcggtc gaaaaaggcg acatgcgaat gcgcatcacg tcgatgccag
     2461 gccgccatgg gccgccccta tccgaactgg tcatgccaga agtgatgggg agcatggtgg
     2521 aattccaacc gatcggcggg tcgtcctttc gcctgtatat caccggcgac acccttatct
     2581 tcgatgacct gaaggaaata ccgcggcgct acagggacat cgatcttgcg ctcctgcatc
     2641 tgggtgggac acgggtgctc gggcttcttg tgaccatgga tgccaagcaa ggagtggaat
     2701 tggtgcagtt ggtgcgtccg cggcaggcga ttccaattca ttacaacgac tacgatgtgt
     2761 ttacgtcgtc attatccgat tttcaacacg aggttgaggc ggcgggattg cgtgaacgaa
     2821 tccactactt gagccacgga gacacgtaca ggtttcggcc ttcttcaaaa cgggcggctg
     2881 cctgatgagg ccagggcagc cgtccgacga tgactagagg atgcgccagt ttctatgaac
     2941 gcgctcccgt tttagtctgg cccaatccga cgtgcaggag caacgcatga agcctcgcaa
     3001 gcgagcgagc aacaagccga ggaaggccaa aacgtcggag ttggagcggg acgaggagta
     3061 tcgggccgtc atcgagcccg atcctcttct gaaagaggta ggggaggcaa atgaacgcat
     3121 cgccgatcct gccgaacgcg aacgcgtcgt cccgagtagg ccttgcaaat aggcgagtct
     3181 cggccgtcaa ctgccatgcg tagctccagt ctcgttcgag tgttttctac cacatcatgg
     3241 tgttgagcag ccgtgtacac gttgggtatt aatgcagcgt ttcacgatcc ggccgcctgt
     3301 ctggtgcgcg atggggtcgt gatcgcggct gccgaagaag agcggttcac acgtatcaag
     3361 cacggtaagc gtcccatccc cttcactacc tacgaattgc ccttccacgc tgtcgagttt
     3421 tgcgtcaggc aggccggcat ccgattgacg gacgtcgatc acgtggccta ttccttcgat
     3481 ccttttctac tgctcggccg ccatgccggc gcatcgcaga ttgcgattcc gctccacggc
     3541 accggctccg agagcaacga atgggagtct ccctgggatc cgctcgcgtt gtcggccatc
     3601 gtcaatgcgc cacggcatct cgccagcggc gtcccgcatc atctcggtga acggttaagc
     3661 ggtcttgccg agtcaggtcc cgcgcgctgg cactatgtgc cgcatcatgt ggcccacgcg
     3721 gccagcgcct ttcatgcgtc accgtatgag acggctgcgg tgatgacgct tgacggacgg
     3781 ggcgagcggg cgacgacgag ttatcacgtc gggaagggac aggatttgac agagcacgga
     3841 gccgtgacca tgccgcattc gctgggtctt ctgtacgagc aggtgacctc ctacctgggg
     3901 tacttacatt ccagcgacga gtacaaggtc atggcactcg cctcctttgg aaagcctgag
     3961 tacgtcgata tcttgcggaa gagcgtccgt ctgcttgggg gaggtcaata tgtggtggag
     4021 cctctgaacc tcatcgaatt gtgcggtccg cctcgtgcgc ggggtggagc gctcgaacaa
     4081 cgccattata acctggcccg gtcgctgcag caggtcctcg aggagatcac ccttgatctt
     4141 gcgtcctggc tgcacgagtc gactggtgaa ccgagattgt gcctggcggg cggagtggca
     4201 ttgaactgcg tcctcaatgc acgaatacgc tccgcaagtc ctttcaaaga ggtctgggtg
     4261 cagcctgcag ccggcgatgc cggaacggcg cttggcgcag cgctatggat cgatgctcaa
     4321 gtctgtccca cgtccggcgc gcgacgctcg acgatggagc atgtctttct gggaccggcc
     4381 ttcgaggatc aggaaatcga ggcggtactc cggagagcca aagtctctta tcgcagactg
     4441 catcatatcc cggaggatac ggcggagttg ctggaacgag atctcatcgt ggggtggttc
     4501 cagggagcaa tggaatttgg cccccgtgca ctcggagccc gttccatttt agcgtcgccg
     4561 atcgatcctg cgatgcagga tcggctgaac gacattaaag accgtgagga tttccgtccc
     4621 gtagcacccg tggtgctgga ggaagacgca gccgattggt ttgccgatgc cagggtctct
     4681 cccttcatgc tgtttgtgtt cgacgtcaaa tcggacaagg ccatgcggat accggccgta
     4741 cgccacgtcg acgggactgc acgcgtgcag acgatcaacc ggacgcagca tccgctgtac
     4801 tacgatttga tcaacgcgtt caaacgacgc acgggcgttc ccgtgctggt caacacttcg
     4861 ttcaatgtgc gcggtgagcc gatcgtgtgt acgccacgcg atgccctctc ggcatttttc
     4921 tcttcgccgc tcgacgcgct ggttatcgga ccgtttcttg tcacaaaagc tgcggccaaa
     4981 acgccatgaa gatttctgtc gtcattgcga ccttgaatcg gccggtcttg ctcgatcgct
     5041 gtctggcagc gttgtcccgg caaacactgc cacccaccgc atatgaagtt gtcgtcgtcg
     5101 acgacggacc tcaccgggcc accgaagccg tggtacgcag atggagcggc cggagccccg
     5161 ggtttgaggt cgtgtatcgg tgctcgggcc aggctcacgg gccggcggcg gcgcgcaatg
     5221 tcgggtggcg cgccggacga tatccgctca tcgcgttcac agacgacgat tgcgttcccg
     5281 agccaggctg gcttgctgcc gggttgcagg tgttcttacg accagaggtg ggagcagtgt
     5341 ccggccggat tctcgtcccg ctgcctcgca aacccaccga ttgggaacgc aatgtcgccg
     5401 ggctggagcg agctccctgc gcgacggcca attgctttta tcgtaaaacc atactcgagg
     5461 cactgggcgg tttcgatgag cgtttcaccg aagcctggag ggaggacagt gacctccagt
     5521 ttcgcgccct ggcccacggc gtcactatcg tccatgaacc gcaagcccgg gtgctgcatc
     5581 cagtccgtcc agcgccttgg ggcatcagcg tctcgttgca acgccagaac cggtttaatg
     5641 cgttgctcta caagaagcat cccgatctct atagctgctt caatcctcat gagccgccct
     5701 ggaactatta tgtgaccgtc ggctcgatgc tcttcggggg aattgccgct ctttatggag
     5761 cagtacccat ggctacggcg ctctgggtgt gttggctgat catggccagc gcgttttgcg
     5821 tgcggcggct ggtgggggtc acgcatcgtc cgtcgcatgt cgctgaaatg atcatcacgt
     5881 ccttgatcat tcccccctac gccgtgtatt ggcggcttca tggtgccttg aagtatcgag
     5941 tcgcattttt ctaatcgctt gggacggcgc accctactga aggaggtagc ctgatgagtg
     6001 aagatcccaa agacaacaag agggaaatcg atgcggatca aatgattcgt gactatgaag
     6061 aaaccgccta cacggaggag cccgaagagg cgcaaacaga gataccctgg tatgaacagg
     6121 caaagcagga tacctcggtg ccggccgagg cggtgctcac cggtggtgac atcgatgccg
     6181 cctgggacca ggcggcggtt ggcgaagaaa ccgttggagg ttccactccg acgccggacc
     6241 aggacatcgt cgacgaaatc ggtcgtgcgg tcggcgtgaa ctacgaggac agtgagccgc
     6301 tggatcccga ggctaaaatc gagcggcgtg aagagaacag gtgggaatt
//
LOCUS       DASSCQ010000235        16507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U926 k141_114522, whole
            genome shotgun sequence.
ACCESSION   DASSCQ010000235 DASSCQ010000000
VERSION     DASSCQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963098
            Sequence Read Archive: SRR25158419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 02:28:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114522"
                     /isolate="SMAG_U926"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="44.54944444 N 129.7413889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..446
                     /locus_tag="VFH05_14770"
     CDS             213..446
                     /locus_tag="VFH05_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679536.1"
                     /translation="MWNTTEEEAERRRVLLKLAAKGNAQARKELEQEYHAVVYTPAEA
                     AKYVPKERLACLPGSVQRKLDALLHVNHEYDGT"
     gene            895..1041
                     /locus_tag="VFH05_14775"
     CDS             895..1041
                     /locus_tag="VFH05_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679537.1"
                     /translation="MNHVILHDVRAAFFLMMGLLMLATICAVAALSTVMRPVRWADRR
                     SAQF"
     gene            1290..1877
                     /locus_tag="VFH05_14780"
     CDS             1290..1877
                     /locus_tag="VFH05_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET6679538.1"
                     /translation="MKWLQSEGAEGAGSINGSGWHGQGVNPDFPGMSQGGFRNELSGF
                     SQNPSDEYLSHSGGIASVSPATGSMRHRAELSKEEQAAVEAGLKDVFFGFDQWTLSDS
                     GMEALNHNAKWLREHPGAVLKVEGHCDERGTTDYNIVLGDKRSKSARNYLTEIGLSSK
                     QIGIVSYGKERPFCLEHDESCYQLNRRGHVLLKRK"
     gene            1960..2310
                     /locus_tag="VFH05_14785"
     CDS             1960..2310
                     /locus_tag="VFH05_14785"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET6679539.1"
                     /translation="MPLRSQVLKKSRVKEKRAHPRLTAQFRSTFSGGQREGHGKTLDL
                     SDGGCRIETDYPIVPNSSFECRIYAPGLSWPLRIDEAQVRWVQGKTFGLQFVAMQPDE
                     RAKLKQVIEDLKAG"
     gene            2444..2980
                     /gene="msrA"
                     /locus_tag="VFH05_14790"
     CDS             2444..2980
                     /gene="msrA"
                     /locus_tag="VFH05_14790"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6679540.1"
                     /translation="MSEEVATLAGGCFWCLEAVFDQMRGVNAVESGYTGGHQERPTYE
                     AVCGGRTGHAEAVRISFDPAIVSYRELLEAFFVIHDPTTLNRQGHDVGTQYRSAVFYH
                     TPEQKRIAEDVIASLTKERVYPNPIVTEVVAAPAWYEAEPYHQEYFARNPFQGYCTAV
                     VGPKVMKFRTRFSNKLKT"
     gene            complement(2977..3840)
                     /gene="ttcA"
                     /locus_tag="VFH05_14795"
     CDS             complement(2977..3840)
                     /gene="ttcA"
                     /locus_tag="VFH05_14795"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET6679541.1"
                     /translation="MPQTSSPRLPLSRLDDATEQLQTRLLRQVGQTINDYGLIEAGDR
                     VMVCMSGGKDSYGLLDLLLTLQRRAPIHFDIIAVNLDQKQPGYPAHVLPEYLRSAGVP
                     FHIEERDTYSIVKRLIPEGQTTCSLCSRLRRGHLYRIATEQGATKIALGHHRDDILET
                     LLLNLLFTGKLKAMPPKLRSKDGRHVVIRPLAAVREADLIRYAELRAFPIIPCDLCGS
                     QDNLKRKQVKAFLLEWERLAPGCTDSMFASLSNIAPSLLMDHRLFDFRALRRDESLAG
                     DDDAWLDENGS"
     gene            complement(3930..4535)
                     /locus_tag="VFH05_14800"
     CDS             complement(3930..4535)
                     /locus_tag="VFH05_14800"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HET6679542.1"
                     /translation="MGRLILLRHGESQWNLENRFTGWVDVPLSPRGIQEAKSAGDKLR
                     PFRFDRAFTSVLQRANETLRLALEVIGQTSIPTEKDKALNERMYGELQGLNKAETAQK
                     YGEDQVKIWRRSYDVRPPGGESLKDTAERVLPYYEKAIKPHVLAGETILIAAHGNSLR
                     ALVMELEQLSKDQVLELNIPTGAPLLYELASNGTVTSHRYL"
     gene            4663..5487
                     /locus_tag="VFH05_14805"
     CDS             4663..5487
                     /locus_tag="VFH05_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679543.1"
                     /translation="MSTIPSANGPWREVEGWWVRMAERVQVADRAQSFFKAAGAGGLL
                     DRIVQVGGQVDYILFVLMRYWVPTVLPPAGRERVTKGDSDYWLESEHILKAAAARLRE
                     LKPLIELLTTPSPLSEASSGQNVSASPVAELELSNIVEGIAEAAGSYGGPDYTSIVKA
                     FDPVPLRQTQPFKHNKKHSAELWVVFLLREHFRSLGLGKDRYWPLVAPLCTAAGILTQ
                     GGQAYGPDELKSWWQKNWPRTYTQLEQKQESADSGGAAYEQDWSWFQAWISWQRRR"
     gene            complement(5521..6168)
                     /locus_tag="VFH05_14810"
     CDS             complement(5521..6168)
                     /locus_tag="VFH05_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET6679544.1"
                     /translation="MKFCAQCRQQNLDDANFCHQCGEDLNAEQGGSSVSSTPAPTTWS
                     GTDHDRWRDFIGPNADRYLEQFKKFGSPQSPRFALTWNWPAFLFISFLWFLYRKMYLY
                     AAVYAIGPMVSTYLTGDMTSGMVWSVVAGATANYVYYWHCREHIAAIKQQAWADHSKQ
                     HLAMKEAGGVQPYVVWVGVVFYALFFLTMFKMIQEGGLDADKVPPKAKPAIMTSA"
     gene            6299..7510
                     /locus_tag="VFH05_14815"
     CDS             6299..7510
                     /locus_tag="VFH05_14815"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HET6679545.1"
                     /translation="MPTEELKESAEQYLMGTYTRQPISIVRGRGTKVYDLEGREYVDF
                     VGGIAVNILGYGHPDLVLAIQKQAAQLLHTSNLYYTEPQVKLAQMLVDHSFADKVFFC
                     NSGAEANEAAIKLARRYSHDKYGAGRFEIITMKQSFHGRTMATLTATGQEKIQKGYEP
                     LLPGFSYVSFNHLSELEEAVTEKTAAIMLEPIQGEGGVHVASRDYLKAVRDLCTQKDI
                     LLIFDEVQTGMGRTGTLFAYEQFGVQPDVMTLAKGLGGGVPIGACLATMSAAAAFGPG
                     SHASTFGGNPLACAAALAVFRVLLDGRVLDQARRMGEYLSKGLSECRDCHHLVRDVRG
                     LGLLQGMEIEIDTKAVVSDCLKRGILINATGDHVLRFVPPLIISQPEVDRLLDVLSQI
                     FDRHAAQAVAH"
     gene            7523..8494
                     /gene="argF"
                     /locus_tag="VFH05_14820"
     CDS             7523..8494
                     /gene="argF"
                     /locus_tag="VFH05_14820"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET6679546.1"
                     /translation="MTRAFERRPKSVKDLLDVAALSRAEIEALLTLASVLKAKQQGGT
                     SHRLLQGKTLGLLFQKPSTRTRVSFEAGMNQLGGHALVLPMADIQLSRGESVADTARV
                     LSRYLDGIVIRTYDHATVEEWAREATMPVINGLTDLSHPCQALSDLLTIKEKKGTLKG
                     IKIAYVGDGNNVANSLIEATAKMGMTIALGCPSGYQPDQHVVDVARVEAVKTGATIDV
                     GQDPYVAVKEADVVYADVWISMGREREQARRLKVLSPYQVNSRLVAKAKPDAIVMHCL
                     PAHRGEEITAEVLDGPQSVIIDQAENRLHMQKAILTKLLGKRRSGER"
     gene            8491..9702
                     /locus_tag="VFH05_14825"
     CDS             8491..9702
                     /locus_tag="VFH05_14825"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET6679547.1"
                     /translation="MNRGIKKVVLAYSGGLDTSVILKWLQETYEAEVVAFCADLGQGE
                     DLQAVKAKAQKLGVKKIYIEDLRETFVREYVFPMLRGNAMYEGSYLLGTSIARPLIAR
                     RQAEIAIKEGAEAVAHGATGKGNDQVRFELTYMALAPRLKIIAPWREWQMRSRRELIE
                     YAERHGIPVTATKAKPYSMDLNLFHVSYEGGILEDPWEAPPEEIFQMTVSAEKAPNKP
                     VQVEIDYEAGNPVAVDGKRMTPAALLGHLNKLGGAHGIGRVDLVENRYVGMKSRGVYE
                     TPGGTILHVAHRGLESLTMDREVLHFRDSLIPRFADLIYNGYWFSPEREMVQRAIDEA
                     QQDVTGTARVKLYKGSCTLTGRKSKQSLYRLDIATFEEDEVYNQKDAEGFIRLNALRL
                     KIRAQRKKASS"
     gene            9702..11111
                     /gene="argH"
                     /locus_tag="VFH05_14830"
     CDS             9702..11111
                     /gene="argH"
                     /locus_tag="VFH05_14830"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET6679548.1"
                     /translation="MTKDRRANGLANGKAWSGRFREPTHRLVEAFTSSVTVDRRLYAQ
                     DIQGSIAHCKTLEKARVLTQTESRTIVRALETVKLELDRGKFKFSPHDEDIHMAVERR
                     LTELIGPLGGKLHTGRSRNDQVALDVRLYLRHQLEQFAERVADCQRVLVAKAKANRTV
                     AMPGYTHLQRAQPVLFAHHLLAYVDMFERDKGRILDAQKRLNVMPLGSGALAGTNYPV
                     DRRYTAGLLGFPRVTTNSLDAVSDRDFMIEIAAALSITMMHLSRLSEELILWSSQEFR
                     FVDLPEGFCTGSSMMPQKKNPDVPELVRGKTGRVYGQLMNLLTTMKALPLSYNRDLQE
                     DKPAVFDALDTVTTSLEVLTELMRRLKVNREALKQAVQGGGLLATELADYLVTKGIPF
                     REAHAITGRVVRTALERGLELSSLSLQELRQFSERFDQGVFTRLTVEAAIDRKAQIGG
                     TARGQVEQRIRELERMLSS"
     gene            11108..11335
                     /locus_tag="VFH05_14835"
     CDS             11108..11335
                     /locus_tag="VFH05_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6679549.1"
                     /translation="MKVFALACAACVLMGCGTIGSPIPPEDVGLAPLIERQKRQDALD
                     AKKLDAEESQTLMEPQGQDVDLPPLRPVGTR"
     gene            11388..13298
                     /locus_tag="VFH05_14840"
     CDS             11388..13298
                     /locus_tag="VFH05_14840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET6679550.1"
                     /translation="MALIQPMRQGQLLKPVRGKRLLLVDPYPRNSQYHLTASERRAVW
                     FPKLSLPTIAAYTPDSWDVDLVDEAVQDIDFDDPCDMVGLSIMTCYAPRAYEIAKEFR
                     KRGKTVVMGGVHPTYCPDEALQHADAIVCGEAEDLWPRLVADYEAGMMQRVYKMTNFP
                     SLEHYKAPRVELLSPDSYMTRQCSFTTRGCHFDCEFCSVSPFNGKTTRRRPVPEVITE
                     LQNVQRWIRSELVERIRDEPLWNAFLAALKIRIGLDDGSIVAFVDDLHNSNRAYCREL
                     WTALKPLKIKWGCQSTLFLGDDEEMVKLAAESGCVSVFVGMESLDEDSLDETNKPFNR
                     VQKFSQEIQMFHKYGIMVNPGIVFGFDNDDEAVFERAVDFLKKNQVELAYFNVLTPLP
                     GTALFDRYNNAGRIFDRDWSKYDGKHVVFQPSRMTPEQLQEGFYWANHQFYSLPNIWY
                     RLSGTKQRFIARWEMNREFRKLVKRSCPKGRLSPLASVLKNLQAKLPVLDTDHLIPSA
                     LHALKQRFDPKAPSTQQLTLNIKVKRHDKFAALFMDLEGAIDRLNVHELINRIREAAE
                     KAKMDIIVNFENLKLAAPDALKALADSEAIKAAVPNVKVRYRKFRDAFETSMQELSLS
                     GIEMLTEDLQDA"
     gene            13291..14553
                     /gene="lysA"
                     /locus_tag="VFH05_14845"
     CDS             13291..14553
                     /gene="lysA"
                     /locus_tag="VFH05_14845"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET6679551.1"
                     /translation="MHNFEYRQGELYCEQVPVSQIAKETGTPCYIYSHATLTRHIRAY
                     DSAFKSIPHLIAFAMKANSNLAILRLMAKEGSGVDIVSGGELFRAIKAGVPPSKIVFA
                     GVGKNAEEIRDALKADILMFNIESSAEMHALNDVAASIGRKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRALEEFRIASTLRHIEVVGVHAHIGSQLTEVTPFVESLKKVI
                     ALIEALKGQGTNIRYLNIGGGLGITYSDEKPPLPHELADAVSPLVRNLDLTLVMEPGR
                     VIVGNAGILVTKVLYEKIGEAKRFIIVDAAMNDLIRPSLYNAYHDIRPVSEALLHRPK
                     HVVDVVGPVCESGDFLAKDRTLPDVKPGDLLAVMSAGAYGFVMASNYNSRPRVPEVLV
                     KDGEVHVIRTRENYDDLIKGESIPAFLN"
     gene            14561..15463
                     /gene="dapA"
                     /locus_tag="VFH05_14850"
     CDS             14561..15463
                     /gene="dapA"
                     /locus_tag="VFH05_14850"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET6679552.1"
                     /translation="MSSTSVCRGMMFTGSLVAIVTPFRKGKVDERAFGDLIEWQIANG
                     TNGIVPCGTTGESATLTHEEHHRVVRLTVEAVRRRVPVIAGSGSNSTDEAISLTRHAK
                     EAGADGALLITPYYNKPTQEGLYRHYKAVAEAVDLPQILYNIPSRTGVNMLPATVARL
                     AAMKNVVGVKEGSGSVQQASEIAQTCGERITVLAGDDALTLPMMAVGGKGVITVTANV
                     MPRDMAQLVAAFTAGRIDEAREIHFRLSPLFAALFYETNPIPVKEALGMMGRIDPELR
                     LPLCSMGNDNRNQLARVMKEMRLI"
     gene            15492..16295
                     /gene="dapB"
                     /locus_tag="VFH05_14855"
     CDS             15492..16295
                     /gene="dapB"
                     /locus_tag="VFH05_14855"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET6679553.1"
                     /translation="MINVIVAGATGRMGSRLIALIKDSTALTLAGAIESKGHHALGED
                     AGEATGSGKTGMPVTDDLAALLEKGEVVIDFSVPEATLSHLRAAVQHRRAMVIGTTGF
                     SSDELQELKSLARQVPCVFSPNMSVGVNLLYKVIGEMAKTLGDDYDIEVIEAHHRLKK
                     DAPSGTALKIAEVLARAVNRDLNQVGVYARKGLVGERKKQEIGIQTIRAGDIVGDHTV
                     LFGGMGERIEITHRASSRDTFARGALRAARWVVKQPPGLYDMMDVLNLK"
BASE COUNT         3751 a         4414 c         4900 g         3442 t
ORIGIN      
        1 cgggcgaagg gcatgtcggg tccgctggcg tagcgattgg gctccactgg ggatgttacc
       61 catgttgata agcctcataa ttttgtcgtt ttttatgccc ttaaacgagg ctgtcgaacg
      121 ggcgaaggaa gtcgagcgca atgcagttaa tcaacagcaa caggcaccta tgatcggggc
      181 cctcgtagag ccttagccct ggaaggatta atatgtggaa tacaactgaa gaagaagcgg
      241 aacggcgtcg tgtcttattg aagttggctg cgaaagggaa cgctcaagca cgcaaagaat
      301 tggaacaaga ataccatgca gtcgtgtata caccggctga agccgcaaag tatgttccga
      361 aggagcgcct ggcctgcctg cctggttcgg tacagcgaaa attggacgcc ttgctgcatg
      421 tgaaccacga gtatgatggg acataatcgg gaggcacatg tagtcggttg tacaggtgtc
      481 atgtcgtgac gagacccgcc acttcaagcg ctcgcgcctg aaagtctgat ttctgataac
      541 cgaagtaagc tcatgaactt taattccact tcaagtactt agccctcgtc gtcacgggct
      601 ggctcagccc ctccccactt aagcagcatg gatttttgac ctatcccgtt gtaggctaaa
      661 agaggttcat gaagaccaga ggagctcgga agtggtattc tatggacaac gcctgatgag
      721 acatcccgat gggacgccaa gcggatgcac tgatcgtggc agcaggcaag tatggacaag
      781 gcattgcccc tgttgcggat gctggttgac gaaagttgat cccatgaccc ggtggctctg
      841 ccgttgtggc tggcaaagcg ggtaggaacc gattcaacga gaagggaaat ttcaatgaat
      901 catgttatcc tccatgacgt aagggccgca ttcttcttga tgatgggatt gctcatgctg
      961 gccacgatct gcgcagtcgc agccttgagc acggtgatgc gtcccgtgcg ctgggcggat
     1021 cggcgttcgg cgcagttcta gcggtactgc gaaacaccgg ttctgccttg tagggcctcc
     1081 ttccggccgc tgctagaaaa cttcatatat tccagcgtct tcgcatatct cttccagtaa
     1141 catccatctt gtaaatccgg tgctatagtt gttgcagtga gtaaactgcc acaacagggg
     1201 gatgggggag caccatgaag gccaatctca ggaagagcgt tgtaatagcg gctttgggtc
     1261 tgaccgtgtt cgctacggct ggatgcggga tgaagtggtt acagtccgaa ggcgctgaag
     1321 gggccggttc aatcaatggc tcaggatggc atggccaagg cgtcaatccc gatttcccgg
     1381 gtatgagtca aggcggcttt cgcaacgagt tgagcggatt ttcccagaat ccatcggatg
     1441 aatatctttc tcatagtggc ggcattgcct ccgtctcacc ggccacaggg agcatgcgtc
     1501 atcgtgcgga attgtcaaaa gaggagcaag ccgctgtgga ggccggtctg aaggatgtct
     1561 tctttgggtt tgatcagtgg acgctgtcgg attccggaat ggaggccctc aatcacaacg
     1621 ccaagtggct gagagagcac cccggagcgg tgttgaaggt tgagggccac tgcgatgaac
     1681 gggggacgac tgattacaac atcgtactcg gcgacaaacg atcgaagagc gcccggaatt
     1741 acctcactga aataggtctc agtagcaaac agatcggcat cgtgtcctat ggaaaggaac
     1801 gcccattttg cctggaacat gatgaatcct gctatcaact caaccgccgc ggacacgttc
     1861 tgttaaaacg aaaatagtca cgactcaaag acagtgagtc accccgtcgc atgctcatca
     1921 tgagagccgt cggggttcgt catttgagag gacacatcga tgccgttacg atcccaggtg
     1981 ctcaaaaaat ctcgcgtgaa ggaaaaaagg gcgcatccga gactgactgc gcagttccgc
     2041 agcacgtttt ccggtgggca acgcgagggt catgggaaaa cactggatct atccgatgga
     2101 ggctgtcgca ttgaaaccga ttaccccata gtgccgaata gttcattcga atgccggatc
     2161 tacgcacccg gtttgagttg gccattacgc attgatgagg cgcaagtgcg atgggtccag
     2221 ggcaagacat ttggccttca attcgtggct atgcagccgg atgaacgggc aaagttgaag
     2281 caagtcatcg aggatctgaa agccggttag acggcaccac ccctgctcca ttctcaattc
     2341 ccgcccttgg tctctgctcg ccggagtttc gtttcatgtg atatataagg acgaggcgtc
     2401 gccatgacgc atggttcacg accgatcaca cagcagggga gccatgagcg aagaggttgc
     2461 gacacttgcg ggaggatgct tctggtgtct ggaagcggtc ttcgaccaga tgcgaggtgt
     2521 caacgccgtc gagtccggct atacaggcgg tcatcaagag cgccccacgt acgaggctgt
     2581 gtgcggcggc cgtaccggtc atgcggaagc cgtccgcatt tcgttcgatc cggctatcgt
     2641 cagttatcgg gagttactgg aggcgttttt cgtgattcat gatccgacca cgctcaaccg
     2701 tcaagggcac gatgtcggca cgcagtaccg gtccgcggtc ttttaccaca ccccggagca
     2761 gaagcgaatt gcggaagacg tgatcgcatc cctcaccaag gagcgggtct atcccaaccc
     2821 gatcgtgaca gaagtggttg ctgctcccgc atggtatgaa gccgagccgt accatcagga
     2881 atatttcgcg cggaatccct ttcaaggcta ctgtactgcc gtcgtcgggc cgaaggtgat
     2941 gaagttccgc acacgctttt cgaacaagct taagacctag ctgccgtttt catccaacca
     3001 ggcatcgtcg tcgccggcca acgattcgtc gcggcgcaag gcccgaaaat caaagagtcg
     3061 gtgatccatc agcagcgacg gagcgatgtt ggacaacgag gcgaacatgc tatccgtgca
     3121 accgggggct agacgttccc attccagtaa aaatgctttc acctgtttgc gtttgaggtt
     3181 gtcttgtgac ccgcacagat cgcagggaat gatgggaaac gcgcgcagtt cggcataacg
     3241 gattagatcc gcttcccgga cggcggcgag cgggcggatg accacatgcc gaccgtcttt
     3301 ggaacgtagc tttggcggca tggctttgag cttcccggtg aagagaagat tcaacagcag
     3361 cgtttcgaga atgtcatcac gatggtgtcc cagcgcgatt ttcgtggccc cttgttctgt
     3421 ggcgatgcgg tagagatgac ctcgacgaag ccgggagcac aaggagcagg tggtctgtcc
     3481 ttctggaatc agtcgtttga cgatggaata cgtatcgcgc tcttcaatgt ggaacggaac
     3541 gccggcggac ctcagatact cgggcaggac atgggcggga tatccgggtt gtttctgatc
     3601 caagttgacc gcaatgatat cgaagtgaat aggcgcccga cgctggagcg tcagtaaaag
     3661 gtcgagtagc ccgtaactgt ccttcccccc ggacatacag accatcaccc tatcgcctgc
     3721 ttcgatcaag ccataatcgt tgatcgtctg accgacctga cgcaggagac gtgtttgcaa
     3781 ctgttcggtt gcatcatcca acctggacag cggcaaacgg ggggaggatg tctgtggcat
     3841 aggacgcatt gtacgcgttc gcacgatcgg gtgtcgattg tgggtacggc tgagctcggt
     3901 caggtggcga cggtcatgcg agacggggtt tacaaatatc ggtggctggt gacggttccg
     3961 ttcgaggcta actcgtagag caatggcgcg ccagttggaa tattcaattc caggacctgg
     4021 tccttggaaa gttgttccaa ttccatgaca agggcgcgca gactgttgcc atgtgcggcg
     4081 atgaggatgg tctcacccgc gaggacatgc ggcttgatgg ctttctcata atagggcaag
     4141 acacgttcgg cggtgtcctt cagactttct cctccgggcg gccggacgtc atagctccgt
     4201 cgccatatct tcacttgatc ctcgccgtat ttttgagcag tctccgcttt gttcaatcct
     4261 tgcagttcgc cgtacatccg ctcgttcaag gccttgtcct tttccgtcgg aatcgaagtt
     4321 tggccgatca cctcgagtgc caggcgaagg gtctcattcg ctcgctgcaa gacggatgtg
     4381 aaggcacggt cgaagcggaa gggacgaagc ttgtcgccgg cgcttttcgc ttcctgtatt
     4441 cctcgtggtg agagcgggac atcgacccat cccgtaaacc ggttctcgag attccactgg
     4501 gattcgccgt gacgcaagag gattagtcgg cccatccatt ccctccgagc aagtgaatca
     4561 acgtgtggga ccaagaatac tggaacagga ctccaagtca agcgttgctt tcgagctaat
     4621 gcggcagtac catgccgcac gatttcaccg gaaagtcctt cgatgagtac gattccgtcg
     4681 gccaacggtc catggcgcga ggttgaaggc tggtgggtgc gcatggccga acgggtacag
     4741 gtggctgacc gcgctcaatc gtttttcaag gcagcgggag ccggcggcct tcttgaccgc
     4801 attgtgcagg tcggaggcca agtcgattac attctctttg tcctcatgcg ctactgggtg
     4861 ccgacggtgt tgccgccggc aggccgcgag cgcgtgacca aaggcgacag cgactactgg
     4921 ctcgaatcgg agcacatact caaggccgct gccgcccgtc tgcgggaact aaaaccctta
     4981 atcgagttgc tgaccactcc cagcccgctt tcggaggcat cgtccggtca gaacgtcagc
     5041 gcctctccgg tggcggagtt ggaattatcg aacatcgtcg aaggcatagc ggaagcggcg
     5101 ggtagctatg ggggaccgga ctatacctcg atcgtgaagg catttgatcc cgtccccttg
     5161 cgtcagacgc aacccttcaa gcacaacaag aaacatagtg cagaactttg ggtggtgttc
     5221 ttgttgcggg agcattttcg tagtctcggg cttggaaagg accgctactg gccgctcgtc
     5281 gcgccgctgt gtactgctgc aggtattctg acccagggcg ggcaggccta tgggcccgat
     5341 gaactgaaga gctggtggca gaaaaactgg ccccgcacat atacccagct ggaacagaag
     5401 caggagtcgg ccgattcggg cggagctgca tacgagcaag actggagttg gtttcaggca
     5461 tggatctcgt ggcagcgccg ccgctgactt ggcccgatcc tgacttcggc caacgggatg
     5521 ctaggcgctc gtcataatag cgggcttggc tttcggtgga actttatcgg cgtccagacc
     5581 tccttcctga atcattttaa acatcgtgag gaagaacaac gcatagaaga cgacgcccac
     5641 ccatacgaca tacggttgta ccccgccggc ttccttcatg gccagatgct gcttcgaatg
     5701 gtcagcccag gcttgttgct tgatcgccgc gatgtgctca cggcaatgcc aataatagac
     5761 ataattcgcc gtcgctccgg ctacgacgct ccagaccatg ccgctcgtca tgtcccccgt
     5821 caggtaggtg gaaaccatgg gccctatcgc gtacacggcg gcatagagat acatcttccg
     5881 atagagaaac cacagaaacg agatgaacag aaaggctggc cagttccagg ttaaggcgaa
     5941 ccggggggat tgcggcgagc cgaatttctt gaactgttcg aggtaacggt ccgcatttgg
     6001 accgataaag tcacgccagc gatcatgatc ggtgccgctc cacgtcgttg gggcaggcgt
     6061 tgaggacacg gaagaccccc cctgctcggc attgagatcc tcgccgcact gatgacaaaa
     6121 atttgcgtcg tctagatttt gctgtcggca ctgcgcgcag aatttcatcg ctcagaggtt
     6181 accacatcgt tctgcgggca tggctggaaa ggcgccgggc gggtggtttc ccagttgatt
     6241 tgctttcatc atcactcctg tgctaggttc ccttacttta caaggggata cgtctgtcat
     6301 gccgacggaa gaactcaagg aaagcgcaga acaatatcta atggggacgt acacccgtca
     6361 accgatctca attgtccgtg gacgaggaac gaaggtgtat gacctggagg gccgtgaata
     6421 cgtcgatttc gtcggaggaa tcgccgtcaa tattctgggc tatggtcacc ccgatctcgt
     6481 tctggccatt caaaaacagg ccgcacaact tctccacacc tcaaatctat actacacaga
     6541 accgcaagtg aagcttgcgc aaatgctcgt ggaccactcg ttcgccgata aagtgttttt
     6601 ctgcaatagc ggcgcagagg ccaatgaagc cgcgatcaag ctggcgcgcc gatattcaca
     6661 cgacaagtat ggcgccggcc gctttgaaat tattacgatg aagcaatcgt tccatggccg
     6721 cacgatggcg acattgacgg cgactggtca ggaaaaaatt cagaaggggt atgaaccgct
     6781 tctcccgggc ttctcctatg tctccttcaa tcacttgtcc gagctggagg aggctgtgac
     6841 tgaaaagaca gcagccatca tgctggaacc gattcaagga gaaggaggtg tccacgttgc
     6901 ctcccgcgat tatctcaaag cggttcgcga cctgtgcacg caaaaagata ttctgctgat
     6961 tttcgacgaa gtgcaaactg gtatggggcg cacggggacc ctgttcgcgt acgagcaatt
     7021 cggggtccaa ccggatgtga tgacgcttgc gaaaggtctt ggtggcggcg tcccgatcgg
     7081 ggcctgtctc gcgacgatgt ctgccgcggc tgcattcggc cccggctcgc atgcatcaac
     7141 gttcggcggc aacccactgg cctgcgcggc agcgctcgca gtcttccgcg tgctcctcga
     7201 tggtcgtgtc ctcgatcaag ctcgccgcat gggcgaatac ctcagtaagg gcttatcgga
     7261 atgcagggat tgccatcatc tcgtgcgcga cgtccgcggg cttggcctcc tgcagggtat
     7321 ggaaatcgag atcgatacca aagcagtcgt tagcgactgc ctgaagaggg gcattctgat
     7381 caacgcgacc ggagaccacg tgctccgttt cgtcccaccg ttgattatca gtcaaccgga
     7441 agtcgatcgt ctgctcgacg tgctttcgca gatcttcgac cgacacgcgg ctcaggccgt
     7501 ggcgcactaa ggtcgctagc ccatgacacg agcgtttgag cgaagaccca agtcggtcaa
     7561 ggacttgttg gacgtggctg cgctgtccag ggccgagatc gaagcgctgt tgacattggc
     7621 cagtgtgctc aaagccaaac aacagggagg gacctcgcac cgcctgcttc aaggcaaaac
     7681 gctcggcttg ctgtttcaaa agccttccac gagaacgcgt gtctcgtttg aagcaggaat
     7741 gaatcaatta gggggccatg cattggtgct gccgatggcg gacattcagc tgtcgcgcgg
     7801 tgaaagcgtg gcggacacgg cgcgcgtgtt gtcgcgctat ctcgatggaa tcgtcatccg
     7861 gacctacgac catgcgacgg ttgaggagtg ggcccgggag gcgaccatgc ccgtcatcaa
     7921 cgggctcacc gatttgagcc atccctgcca ggccttgtca gacctcctga cgatcaaaga
     7981 gaagaaggga acattgaaag gcattaagat cgcgtatgtc ggggacggca acaatgtggc
     8041 gaattctctc atcgaggcca cggccaagat gggtatgacc attgcgctgg gctgcccatc
     8101 cggctatcag ccagatcaac acgtggtcga tgtggcacga gttgaagccg tcaagacggg
     8161 agcgactatc gatgtcggcc aagacccgta cgtcgcggta aaggaagcgg acgtcgtcta
     8221 cgccgatgtg tggattagca tgggccgcga gcgggaacag gccagacggc tgaaggtcct
     8281 ctcaccgtat caggtgaaca gccggcttgt tgccaaagcc aagcccgacg cgatcgtcat
     8341 gcattgtctg ccggctcacc gcggagaaga gatcacagcc gaggtgctcg atgggccgca
     8401 gtcggtgatc atcgatcagg ctgaaaaccg cctgcatatg cagaaggcca tcctgacgaa
     8461 gctgttggga aaaaggagat caggcgaacg atgaaccgtg gaatcaagaa agtcgtgctg
     8521 gcctattccg gcgggctgga tacctccgtc attttgaaat ggttgcagga aacgtacgag
     8581 gcggaggtgg tcgccttttg cgccgatctt ggtcaggggg aagatctgca ggccgtgaag
     8641 gccaaggccc agaagctcgg cgtcaagaaa atctacattg aagacctgcg agagactttt
     8701 gtcagggagt atgtgttccc catgcttcgc gggaacgcga tgtatgaagg ttcctatctg
     8761 ctgggcacgt cgattgcccg tccgctgatt gcccgtcgcc aggcggaaat cgcgataaaa
     8821 gaaggtgcgg aggccgttgc ccacggcgcg acgggaaaag gaaacgacca ggtccgcttt
     8881 gaactgacgt atatggcgct ggccccccgc ttgaaaatca ttgctccgtg gcgcgagtgg
     8941 cagatgcgtt cacgccggga gttaatcgag tacgccgagc gccacgggat cccggttacg
     9001 gcgacgaaag ccaagccgta tagcatggac ctcaatttgt tccacgtgag ctatgagggc
     9061 ggcatcctcg aggacccctg ggaggcaccg cctgaggaaa tcttccaaat gacggtctcg
     9121 gcggagaagg cgcccaataa gcccgtgcaa gtggagattg attatgaggc aggcaatcct
     9181 gtcgcggtgg acgggaaaag gatgactcca gcggcgttgc tgggccatct caacaagttg
     9241 ggcggtgcac atgggatcgg ccgtgtcgat ttggttgaaa accgctatgt cgggatgaaa
     9301 tcgcgcggcg tctatgagac gccgggaggc acaatcctcc atgttgccca ccgaggcctg
     9361 gaatcgctga cgatggatcg agaggtcctg cactttcgtg acagtctgat cccgcgtttt
     9421 gcagatttga tttacaacgg gtattggttc agcccggagc gcgagatggt gcagagagcg
     9481 atcgatgaag cacaacagga cgttaccggc acggctcgcg tgaaactgta taaagggagt
     9541 tgtaccttga ccggacggaa atcgaaacag tcgctgtatc gtctcgacat tgcgaccttc
     9601 gaagaggatg aggtgtacaa ccaaaaagat gccgaaggct ttatccgcct caatgccttg
     9661 cggctgaaga tccgggcaca acgaaaaaag gcctcgtcct gatgaccaaa gaccggcgag
     9721 cgaacggact ggccaacggc aaggcttgga gcgggcgatt tcgcgaaccg actcaccgct
     9781 tggtcgaagc gttcaccagc tcggtcaccg tcgatcgcag gctgtacgca caggatattc
     9841 agggcagcat cgcccattgc aagacgttag aaaaggcacg agtgttgact cagacggaga
     9901 gccgcacgat cgtgcgcgcc ctggaaacgg tcaagctcga gttggatcgc gggaaattca
     9961 aattctcgcc ccatgacgag gacatccaca tggcagtcga gaggcggctc acggagctga
    10021 tcggaccatt aggaggtaaa ctccatacgg gacgcagccg caacgaccag gtggcgttgg
    10081 acgttcgact ctatctccgt catcagcttg agcaattcgc agaacgggtg gcagactgcc
    10141 agcgcgtcct tgtggccaag gctaaggcca accggacggt agcgatgccc gggtacacgc
    10201 atcttcaacg ggctcagccg gtgctgttcg cccatcatct gctcgcatac gttgatatgt
    10261 ttgaacgcga taagggacgg attctggatg cgcaaaagcg attaaacgtg atgccgctcg
    10321 ggtccggcgc cttggcaggg acaaactatc cggtcgaccg gcgctacacg gccgggctcc
    10381 tggggtttcc acgagtcacc accaacagcc tggatgcggt atccgaccgg gacttcatga
    10441 tcgaaattgc ggctgcgctg tcgattacga tgatgcatct gtcgcgcttg agcgaagagc
    10501 tcatcctctg gtcgtcccag gaatttcggt tcgtcgatct accggagggg ttttgcaccg
    10561 gcagcagcat gatgccgcag aaaaagaatc cggatgtgcc tgagctggtc cggggcaaaa
    10621 ccggccgcgt ctatggccaa ttgatgaatc tcttgacgac catgaaggcg ctccccttga
    10681 gttataatcg agacctgcaa gaggataagc cggcggtgtt cgatgcgttg gacacggtca
    10741 cgacgtcgct ggaagtcttg acggaattga tgcgtcggct caaagtgaac cgtgaggccc
    10801 tcaaacaggc ggtgcaaggc ggtggccttc tggcaaccga actggcggat tacctagtaa
    10861 ccaaaggcat cccattccgc gaagctcacg ctattaccgg ccgcgtcgta cgaacggcac
    10921 tcgaacgggg cctggagctt tcgtctttgt ccttgcagga gttgcgacag ttttccgagc
    10981 ggtttgacca gggggtattc acccggttaa ccgtggaagc cgcgatcgat cgtaaagcgc
    11041 aaatcggagg cacagctcga ggccaggtag agcagcggat aagagaattg gagcggatgt
    11101 taagctcatg aaggtcttcg cgcttgcatg cgccgcctgc gtcttgatgg gctgtggaac
    11161 gatcgggtcg ccgattcctc ccgaggacgt cggtttggcg ccgttgattg aacgccaaaa
    11221 gagacaggac gcattggacg ccaagaagct tgacgctgag gaatcgcaga ctctcatgga
    11281 accccagggt caagatgttg acctcccgcc attgcggccg gtggggacgc gctagggtta
    11341 cgtaacacac attttttgca ctcattacag aagtggagga gcgactgatg gcactaatcc
    11401 aaccaatgcg acaagggcaa ttgctgaagc cggtgcgagg caaacggctc ctgttggtcg
    11461 atccttaccc gcgcaatagc cagtaccatc tgacggcgtc cgagcgacga gctgtctggt
    11521 tcccgaagct gagcctgccg accattgcag cgtacacccc cgactcctgg gatgtagatc
    11581 tcgtcgatga agccgttcaa gacatcgatt ttgacgatcc atgcgacatg gtcggcctct
    11641 ccatcatgac ctgctatgcg ccacgagcct acgaaatcgc aaaggaattc cggaagcggg
    11701 ggaaaaccgt cgtgatgggc ggggtccacc ccacgtattg tcctgacgaa gccctgcagc
    11761 acgcggatgc gattgtctgc ggtgaggccg aggacttgtg gcctcgcctt gttgccgatt
    11821 atgaagccgg gatgatgcag cgcgtgtaca agatgacgaa cttcccttct ctcgagcact
    11881 ataaggcccc gcgtgtcgaa ttgttgagtc cggattccta catgaccaga caatgcagct
    11941 ttacgacgag aggctgccac ttcgactgtg agttttgcag tgtgtcgccg tttaacggca
    12001 agacgacgag gcgtcggccg gtccccgagg tcatcacgga actccagaac gtacaacgat
    12061 ggatccggag tgagctcgtt gaacggatcc gcgacgaacc gctctggaat gcgtttctcg
    12121 ctgccctgaa aatacgaata gggcttgatg atggaagcat cgtcgcgttc gtcgacgacc
    12181 tgcacaacag caatcgggcg tactgccggg aactctggac cgctctgaag ccgctcaaga
    12241 tcaagtgggg ctgtcaatcc accctgttct tgggggacga cgaagagatg gtaaagctgg
    12301 ctgcggaaag cggttgtgtc tctgtgttcg ttggcatgga atcgctcgac gaagacagtc
    12361 tagatgagac gaacaagccg ttcaatcggg tgcagaagtt ttctcaggaa attcagatgt
    12421 tccataagta cggcatcatg gtgaaccccg gtatcgtgtt tgggttcgac aatgatgatg
    12481 aggcggtatt cgaacgagcc gtcgatttcc tgaagaaaaa ccaggtggag ctcgcctact
    12541 ttaatgtgct gactccgttg ccaggcacgg cgctgttcga tcggtataac aatgcgggtc
    12601 gcattttcga ccgtgattgg tcaaagtatg acggcaaaca cgtcgtgttt cagcccagcc
    12661 ggatgactcc tgagcaactt caagagggat tttactgggc caaccaccaa ttttattcac
    12721 tgccgaacat ttggtatcgg ctgtccggca cgaaacagcg gttcatcgca cgatgggaaa
    12781 tgaaccggga attccgcaag ttggtgaaac gcagctgtcc caaagggaga ttgtctccgc
    12841 tcgcctccgt actcaagaat ctccaggcca agctgcccgt actggatacc gatcacctga
    12901 tccccagcgc cttgcatgcg ctcaagcagc ggttcgaccc caaggcaccg tccactcaac
    12961 agttgacgct caacatcaag gtcaaacgac atgataagtt tgccgctctg ttcatggatc
    13021 tcgagggcgc aatcgaccgg ctgaacgttc atgaactcat caatcgaatc agggaagcgg
    13081 ccgaaaaagc caagatggac atcatcgtca acttcgaaaa tctgaagctc gcggccccgg
    13141 atgcgctcaa ggcgctcgcc gattccgaag cgatcaaagc cgcggtgccg aacgtgaaag
    13201 tccgataccg gaagtttagg gacgccttcg aaacctcgat gcaggagttg tctctttcgg
    13261 gcatcgaaat gctgaccgag gatctgcagg atgcataatt tcgaatatcg ccagggcgag
    13321 ttgtattgtg agcaggtgcc ggtcagccag atcgccaaag agaccggcac gccctgttac
    13381 atttacagcc acgcgacatt gaccaggcat atccgggcgt atgacagtgc cttcaagagc
    13441 atcccgcacc tcatcgcgtt tgccatgaag gccaattcca atctcgccat tttacgcctg
    13501 atggcgaagg aaggcagcgg agtcgacatc gtatccggcg gcgaattgtt ccgcgccatc
    13561 aaggccggcg tcccaccatc aaaaattgtg tttgcagggg tcggaaagaa tgcggaagaa
    13621 atccgggatg cgctcaaggc cgatattctg atgttcaaca tcgaatcctc ggccgaaatg
    13681 cacgccctca atgacgtggc tgcttcgatc ggcaggaagg cccgggtggc cttacgaatc
    13741 aatccagaca tcgacccgaa aacacatccc tacatttcca ccggcctgaa gaaaagcaag
    13801 ttcggcatcg cggctgatcg tgccctggaa gaatttcgga tcgcctccac tctccggcac
    13861 attgaggtgg tgggcgtcca tgcacacatt ggatcgcaac tgaccgaagt cacccccttc
    13921 gtggaatcct tgaagaaggt gattgcgctc atcgaagccc tcaagggtca agggaccaac
    13981 attcgatatc tcaacatcgg cggcggattg gggatcacct attcggacga aaagcctccc
    14041 ttgcctcacg agttggccga tgccgtgtcg ccgctcgtcc ggaacttgga tctgaccctg
    14101 gtcatggagc cgggccgtgt catcgtcggc aacgcgggga tcctggtgac gaaggtcttg
    14161 tatgaaaaaa tcggcgaagc gaagcgcttc atcatcgttg atgcagccat gaatgacctt
    14221 attcgcccga gcctctacaa tgcttatcac gacatccgtc cggtctccga agcgttactg
    14281 caccgcccca agcacgtggt cgatgtcgtg gggcccgtgt gcgaatcggg tgacttcctc
    14341 gcaaaagacc gcacgcttcc ggacgtcaag ccgggagatt tgctggctgt catgagcgca
    14401 ggcgcctatg gctttgtcat ggcctccaac tacaattctc ggccccgagt tccggaggtc
    14461 ttggtcaaag acggtgaagt ccatgtgatc cgtacccggg aaaattatga cgatctgatc
    14521 aaaggggaaa gcattccagc gtttttaaac tgagcgcggt gtgagttcaa cctccgtctg
    14581 ccgaggaatg atgtttacgg gttcacttgt tgcgattgtg acgccgttcc gcaagggtaa
    14641 ggtcgacgag cgagcattcg gcgatctcat cgagtggcaa atcgcaaacg gcacaaacgg
    14701 catcgtgcct tgcgggacga ccggcgagtc ggccaccttg acgcatgagg agcaccaccg
    14761 cgtcgtcagg ctcacggtcg aagccgtcag gcgccgggtc cccgtgattg caggcagcgg
    14821 ttccaacagc acggatgaag caatttcatt gacgcgccac gccaaagaag ctggggcgga
    14881 tggagcactg ctcattactc cctattacaa caagccgacg caggaagggc tataccgtca
    14941 ctataaggcg gtggcagagg ccgtcgatct cccccagatc ctgtacaaca taccaagccg
    15001 gacaggcgtc aatatgctgc ctgccacagt cgcgcgcttg gcggccatga agaatgtggt
    15061 gggagtgaag gaggggagcg ggtccgtgca gcaggcgtcc gaaattgccc agacctgcgg
    15121 cgagcgcatc accgtgctcg caggggacga tgcgttgacg ttgcccatga tggccgttgg
    15181 cggcaagggc gtgatcacgg tcaccgccaa tgtcatgccg agggacatgg cccagctcgt
    15241 ggcagcgttc acggccggcc gaatagatga ggctcgcgag attcatttcc gcctatcgcc
    15301 tctttttgcg gcgttattct acgaaaccaa tccaatcccc gtgaaggaag cgctgggaat
    15361 gatgggcaga attgatcctg aactgcggtt acccctgtgc tcgatgggaa acgacaaccg
    15421 caatcagtta gcccgcgtca tgaaagaaat gcgactgatc tagtcttgca cagctcaaca
    15481 attcacgtct catgatcaac gtcattgttg ccggcgccac cggccgaatg ggaagccgtt
    15541 taatcgcctt gatcaaggat tcgactgcct tgacgttggc cggcgcgatt gagagcaagg
    15601 gacatcacgc ccttggtgaa gatgccggag aagcgaccgg ttcaggaaag accggcatgc
    15661 ccgtcaccga cgacctcgct gctctactgg aaaagggtga agtcgtcatc gatttttccg
    15721 tccccgaggc gaccctcagc catcttcgag ccgcggttca acatcgtcga gcgatggtca
    15781 ttggcacgac cgggttcagt agcgacgaat tgcaagagtt gaaatcgttg gcgcggcagg
    15841 tgccttgtgt cttctcaccc aacatgagcg tcggagtcaa tcttctctac aaggtcattg
    15901 gggaaatggc caaaacgctg ggagatgatt acgacattga ggtcattgag gcgcaccatc
    15961 ggctcaaaaa ggatgcaccg agtgggactg ctctgaagat tgcagaggtg ctggctcggg
    16021 cggtgaatcg agacctcaat caggtgggcg tctatgcacg aaaagggctg gtaggggaac
    16081 ggaagaagca ggagatcggc attcagacga tacgcgcggg cgacatcgtt ggagatcata
    16141 cggtgctttt tggtggaatg ggcgagcgga ttgaaatcac acaccgggcg agcagccgag
    16201 acacctttgc ccgcggcgca ttgcgtgcgg cccgttgggt agtgaagcaa cccccgggcc
    16261 tctatgacat gatggatgtg ttgaatctca aatgacaatc cagcggatag gctcgcgaga
    16321 caggtgttaa tttaaacgac gagggaaaag aggagaggcg aaacagagcg accagtcaca
    16381 agccgtagca cgaccccgga gactcatgct cgtgagagcg ggtcgaacga tcgttcatcg
    16441 tgtcaatgtc tagccgtaag aggacgaggt tcttgatccg ctgccttccg acgaccacag
    16501 tttaagc
//
